LOCUS       DAHHTG010000001       126542 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.001, whole genome shotgun sequence.
ACCESSION   DAHHTG010000001 DAHHTG010000000
VERSION     DAHHTG010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 126542)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 126542)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..126542
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.001"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            <1..165
                     /locus_tag="NBI74_000001"
     CDS             <1..165
                     /locus_tag="NBI74_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfL family protein"
                     /protein_id="HCD1930390.1"
                     /translation="NKFIKVALVGAVLATLTACTGHIENRDKNCSYDYLLHPAISISK
                     IIGGCGPTAQ"
     gene            complement(273..1358)
                     /locus_tag="NBI74_000002"
     CDS             complement(273..1358)
                     /locus_tag="NBI74_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HCD1930391.1"
                     /translation="MKTFPLQSLTLIEAQQKQFALVDTICRHFPGSEFLTCGDLGLTP
                     GLNQPRITQRVEQVLADAFHAQAAALVQGAGTGAIRAALAALLKSGQRLLVHDAPVYP
                     TTRVIIEQMGLTLITADFNDLSALKQVVDEQQPDAALVQHTRQQPQDRYVLADVLATL
                     RAAGVPALTDDNYAVMKVARIGCECGANVSTFSCFKLFGPEGVGAVVGDADVISRIRA
                     TLYSGGSQIQGAQALEVLRGLVLAPVMHAVQAGVSERLLALLNGGAVAEVKSAVIANA
                     QSKVLIVEFHQPIAARVLEEAQKLGALPYPVGAESKYEIPPLFYRLSGTFRQANPQLE
                     HCAIRINPNRGGEETILRILRESIASI"
     gene            complement(1370..2674)
                     /locus_tag="NBI74_000003"
     CDS             complement(1370..2674)
                     /locus_tag="NBI74_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfT family protein"
                     /protein_id="HCD1930392.1"
                     /translation="MDLYIQIIVVACLTGMTSLLAHRSAAVFHDGIRPILPQLIEGYM
                     NRREAGSIAFGLSIGFVASVGISFTLKTGLLNAWLLFLPTDILGVLAINSLMAFGLGA
                     IWGILILTCLLPVNQLLTALPVDVLGSLGELSSPVVSAFALFPLVAIFYQFGWKQSLI
                     AAVVVLMTRVVVVRYFPHLNPESIEIFIGMVMLLGIAITHDLRHRDENDIDASGMSVF
                     EERTSRIIKNLPYIAIVGALIAAVASMKIFAGSEVSIFTLEKAYSAGVTPEQSQTLIN
                     QAALAEFMRGLGFVPLIATTALATGVYAVAGFTFVYAVGYLSPNPMVAAVLGAVVISA
                     EVLLLRSIGKWLGRYPSVRNASDNIRNAMNMLMEVALLVGSIFAAIKMAGYTGFSIAV
                     AIYFLNESLGRPVQKMAAPVVAVMITGILLNVLYWLGLFVPA"
     gene            complement(2686..3039)
                     /locus_tag="NBI74_000004"
     CDS             complement(2686..3039)
                     /locus_tag="NBI74_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2620 domain-containing protein"
                     /protein_id="HCD1930393.1"
                     /translation="MKKIGVAGLQREQIKKTIEATAPGCFEVFIHNDMEAAMKVKSGQ
                     LDYYIGACNTGAGAALSIAIAVIGYNKSCTIAKPGIKAKDEHIAKMIAEGKVAFGLSV
                     EHVEHAIPMLINHLK"
     gene            complement(3050..3928)
                     /locus_tag="NBI74_000005"
     CDS             complement(3050..3928)
                     /locus_tag="NBI74_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotriesterase-related protein"
                     /protein_id="HCD1930394.1"
                     /translation="MSFDPTGYTLAHEHLHIDLSGFKNNVDCRLDQYAFICQEMNDLM
                     ARGVRNVIEMTNRYMGRNAQFMLDVMRETGINVVGCTGYYQDAFFPEHVATRSVQELA
                     QEMVDEIEQGIDGTDLKAGIIAEIGSSEGKITSLEEKVFIAAALAHNQTGRPISTHTS
                     FSTMGLEQLALLQAHGVDLSRVTVGHCDLKDNLDNILKMIDLGAYVQFDTIGKNSYYP
                     DEKRIAMLHALRDRGLLNRVMLSMDITRRSHLKANGGYGYDFLLTTFIPQLRQSGFSQ
                     ADVDVMLRENPSQFFQ"
     gene            complement(3925..5151)
                     /locus_tag="NBI74_000006"
     CDS             complement(3925..5151)
                     /locus_tag="NBI74_000006"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HCD1930395.1"
                     /translation="MARFVVLVIDSFGVGAMKDVTLVRPQDAGANTCGHILSQLPHLQ
                     LPTLETLGLINALGYAPGDMQPSDSATWGVAELQHEGGDTFMGHQEILGTRPLPPLRM
                     PFRDVIDRVEQALVSAGWQVERRGDDLQFLWVNQAVAIGDNLEADLGQVYNITANLSV
                     ISFDDAIKMGRIVREQVQVGRVITFGGLLTDSQCILDAAESKEGRFIGINAPRSGAYD
                     NGFQVVHMGYGVDEKVQVPQKLYEAGVPTVLVGKVADIVSNPYGVSWQNLVDSQRIMD
                     ITLNEFNTYPTAFICTNIQETDLAGHAEDVARYAERLQVVDRNLARLVEAMQPDDCLV
                     VMADHGNDPTIGHSHHTREVVPVLVYQQGLVATQLGVRTTLSDVGATVCEFFRAPPPQ
                     NGRSFLSSFRFAGDTL"
     gene            complement(5152..6315)
                     /locus_tag="NBI74_000007"
     CDS             complement(5152..6315)
                     /locus_tag="NBI74_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfX family PLP-dependent enzyme"
                     /protein_id="HCD1930396.1"
                     /translation="MFVEALKRQNPALISAALSLWQQGKIAPDSWVIDVDQVLENGKR
                     LIETARLYGIELYLMTKQFGRNPWLAEKLLALGYSGIVAVDYKEARVMRRAGLPVAHQ
                     GHLVQIPCHQVSDAVEQGTDVITVFTLDKAREISAAAVKAGRVQSVLLKVYSDDDFLY
                     PGQESGFVQHSLHEVVAEIQNLPGLHLAGLTHFPCLLWDEAAGKVLPTPNLHTLVQAR
                     DQLAKSGIAIEQLNAPSATSCTSLPLLVEYGVTHTEPGHALTGTIPANQRGDQPERIA
                     MLWLSEISHHFRGDSYCYGGGYYRRGHAQHALVFTPENQRITETYLNAVDDSSIDYTL
                     PLAGEHPVSSAVVLCFRTQIFITRSDVVLVSGIHHGEPEIVGRYDSLGNPLEA"
     gene            complement(6399..6761)
                     /locus_tag="NBI74_000008"
     CDS             complement(6399..6761)
                     /locus_tag="NBI74_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HCD1930397.1"
                     /translation="METRLNLLCDAGVIDKDICKGMMQVVNVLETECHLPVRSEQGTM
                     AMTHMASALMRSRRGEEIEPLDDELLAELAQSSHWQAVVQLHQVLLKEFALEVNPCEE
                     GYLLANLYGLWMAANEEV"
     gene            complement(6778..7683)
                     /locus_tag="NBI74_000009"
     CDS             complement(6778..7683)
                     /locus_tag="NBI74_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1930398.1"
                     /translation="MRRTFIKKEGVVITTLARYLLGEKCGNRLKTIDELATECRSSVG
                     LTQAALKTLESSGAIRIERRGRNGSYLVEMDNKALLTHVDINNVVCAMPLPYTRLYEG
                     LASGLKAQFDGIPFYYAHMRGADIRVECLLNGVYDMAVVSRLAAESYLTQKGLCLALE
                     LGPHTYVGEHQLICRKGESANVKRVGLDNRSADQKIMTDVFFGDSDVERVDLSYHESL
                     QRIVKGDVDAVIWNVVAENELTMLGLEATPLTDDPRFLQATEAVVLTRVDDYPMQQLL
                     RAVVDKHALLAHQQRVVSGEQEPSY"
     gene            complement(7845..8849)
                     /gene="trpS"
                     /locus_tag="NBI74_000010"
     CDS             complement(7845..8849)
                     /gene="trpS"
                     /locus_tag="NBI74_000010"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HCD1930399.1"
                     /translation="MTKPIVFSGAQPSGELTIGNYMGALRQWVNMQDDYHCIYCIVDQ
                     HAITVRQDAQKLRKATLDTLALYLACGIDPEKSTIFVQSHVPEHAQLGWALNCYTYFG
                     ELSRMTQFKDKSARYAENINAGLFDYPVLMAADILLYQTNLVPVGEDQKQHLELSRDI
                     AQRFNALYGDIFKVPEPFIPKSGARVMSLLEPTKKMSKSDDNRNNVIGLLEDPKSVVK
                     KIKRAVTDSDEPPVVRYDVQNKAGVSNLLDILSAVTGQSIPELEKQFEGKMYGHLKGE
                     VADAVSGMLTELQERYHRFRNDEAFLQQVMKDGAEKASAHASRTLKAVYEAIGFVAKP
                     "
     gene            complement(8842..9600)
                     /gene="gph"
                     /locus_tag="NBI74_000011"
     CDS             complement(8842..9600)
                     /gene="gph"
                     /locus_tag="NBI74_000011"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HCD1930400.1"
                     /translation="MNKFEDIRGVAFDLDGTLVDSAPGLAAAVDMALYALELPVAGEE
                     RVITWIGNGADILMERALTWARQERATLRKTMGKPPVDDDIPAEEQVRILRKLFDRYY
                     GEVAEEGTFLFPHVADTLGALQAKGLPLGLVTNKPTPFVAPLLEALDIAKYFSVVIGG
                     DDVQNKKPHPDPLLLVAERMGIAPQQMLFVGDSRNDIQAAKAAGCPSVGLTYGYNYGE
                     AIDLSQPDVIYQSINDLLPALGLPHSENQESKND"
     gene            complement(9593..10270)
                     /gene="rpe"
                     /locus_tag="NBI74_000012"
     CDS             complement(9593..10270)
                     /gene="rpe"
                     /locus_tag="NBI74_000012"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HCD1930401.1"
                     /translation="MKQYLIAPSILSADFARLGEDTAKALAAGADVVHFDVMDNHYVP
                     NLTIGPMVLKSLRNYGITAPIDVHLMVKPVDRIVPDFAAAGASIITFHPEASEHVDRT
                     LQLIKENGCKAGLVFNPATPLSYLDYVMDKLDVILLMSVNPGFGGQSFIPQTLDKLRE
                     VRRRIDESGFDIRLEVDGGVKVNNIGEIAAAGADMFVAGSAIFDQPDYKKVIDEMRSE
                     LAKVSHE"
     gene            complement(10288..11124)
                     /gene="dam"
                     /locus_tag="NBI74_000013"
     CDS             complement(10288..11124)
                     /gene="dam"
                     /locus_tag="NBI74_000013"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HCD1930402.1"
                     /translation="MKKNRAFLKWAGGKYPLLDDIKRHLPKGECLVEPFVGAGSVFLN
                     TDFSRYILADINSDLISLYNIVKMRTDEYVQAARELFVPETNCAEVYYQFREEFNKSQ
                     DPFRRAVLFLYLNRYGYNGLCRYNLRGEFNVPFGRYKKPYFPEAELYHFAEKAQNAFF
                     YCESYADSMARADDASVVYCDPPYAPLSATANFTAYHTNSFTLEQQAHLAEIAEGLVD
                     RHIPVLISNHDTMLTREWYQRAKLHVVKVRRSISSNGGTRKKVDELLALYKPGVVSPA
                     KK"
     gene            complement(11231..12517)
                     /gene="damX"
                     /locus_tag="NBI74_000014"
     CDS             complement(11231..12517)
                     /gene="damX"
                     /locus_tag="NBI74_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DamX"
                     /protein_id="HCD1930403.1"
                     /translation="MDEFKPEDELKPDPSDRRTGRSRQSSERSERTERGEPQINFDDI
                     ELDDTDDRRPTRAQKARNEEPEIEEEIDESEDETVDEERVERRPRKRKKAASKPASRQ
                     YMMMGVGILVLLLLIIGIGSALKAPSTSSSDQTASGEKSIDLAGNATDQANGVQPAPG
                     TTSAENTQQDVSLPPISSTPTQGQTPAATDGQQRVEVQGDLNNALTQPQNQQQLNNVA
                     VNSTLPTEPATVAPVRNGNASRDTAKTQTAERPATTRPARQQAVIEPKKPQATVKTEP
                     KPVAQTPKRTEPAAPVASTKAPAATSTPAPKETATTAPVQTASPAQTTATPAAGGKTA
                     GNVGSLKSAPSSHYTLQLSSSSNYDNLNGWAKKENLKNYVVYETTRNGQPWYVLVSGV
                     YASKEEAKKAVSTLPADVQAKNPWAKPLRQVQADLK"
     gene            complement(12609..13697)
                     /gene="aroB"
                     /locus_tag="NBI74_000015"
     CDS             complement(12609..13697)
                     /gene="aroB"
                     /locus_tag="NBI74_000015"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HCD1930404.1"
                     /translation="MERIVVTLGERSYPITIASGLFNEPASFLPLKSGEQVMLVTNET
                     LAPLYLDKVRGVLEQAGVNVDSVILPDGEQYKSLAVLDTVFTALLQKPHGRDTTLVAL
                     GGGVVGDLTGFAAASYQRGVRFIQVPTTLLSQVDSSVGGKTAVNHPLGKNMIGAFYQP
                     ASVVVDLDCLKTLPPRELASGLAEVIKYGIILDGAFFNWLEENLDALLRLDGPAMAYC
                     IRRCCELKAEVVAADERETGLRALLNLGHTFGHAIEAEMGYGNWLHGEAVAAGMVMAA
                     RTSERLGRFSSAETQRIITLLTRAGLPVNGPREMSAQAYLPHMLRDKKVLAGEMRLIL
                     PLAIGKSEVRSGVSHELVLNAIADCQSA"
     gene            complement(13754..14275)
                     /gene="aroK"
                     /locus_tag="NBI74_000016"
     CDS             complement(13754..14275)
                     /gene="aroK"
                     /locus_tag="NBI74_000016"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004709337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroK"
                     /protein_id="HCD1930405.1"
                     /translation="MAEKRNIFLVGPMGAGKSTIGRQLAQQLNMEFYDSDQEIEKRTG
                     ADVGWVFDLEGEEGFRDREEKVINELTEKQGIVLATGGGSVKSRETRNRLSARGVVVY
                     LETTIEKQLARTQRDKKRPLLHVETPPREVLEALANERNPLYEEIADVTIRTDDQSAK
                     VVANQIIHMLESN"
     gene            complement(14676..15914)
                     /gene="hofQ"
                     /locus_tag="NBI74_000017"
     CDS             complement(14676..15914)
                     /gene="hofQ"
                     /locus_tag="NBI74_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA uptake porin HofQ"
                     /protein_id="HCD1930406.1"
                     /translation="MKQWIAALLLMLIPGVQAAKPQKVTLMVDDVPVAQVLQALAEQE
                     KLNLVVSPDVSGTVSLHLTDVPWKQALQTVVKSAGLITRQEGNILSVHSVAWQNDNIA
                     RQEAEQTRAQANLPLENRNITLQYADAGELAKAGEKLLSAKGSMTVDKRTNRLLLRDN
                     KTALSTLEQWVSQMDLPVGQVELSAHIVTINEKSLRELGVKWTLADAQQAGGVGQVTT
                     LGSDLSVATATTHIGFNIGRINGRLLDLELSALEQKQQLDIIASPRLLASHLQPASIK
                     QGSEIPYQVSSGESGATSVEFKEAVLGMEVTPTVLQKGRIRLKLHISQNVPGQVLQQA
                     DGEVLAIDKQEIETQVEVKSGETLALGGIFTRKNKSGQDSVPLLGDIPWFGQLFRHDG
                     KEDERRELVVFITPRLVSSE"
     gene            complement(15826..16230)
                     /gene="hofP"
                     /locus_tag="NBI74_000018"
     CDS             complement(15826..16230)
                     /gene="hofP"
                     /locus_tag="NBI74_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofP"
                     /protein_id="HCD1930407.1"
                     /translation="MRVKRWLLAGIALCLLTGMRDPFKPPEDLCRISELSQWRYQGMV
                     GRGERIIGVIKDGQKKWRRVQQNDVLENGWTILQLTPDALTLGTGTNCEPPHWLWQRQ
                     GDTNEAMDSRTTVDADTRRTGGKAAKSDADGG"
     gene            complement(16220..16660)
                     /gene="hofO"
                     /locus_tag="NBI74_000019"
     CDS             complement(16220..16660)
                     /gene="hofO"
                     /locus_tag="NBI74_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofO"
                     /protein_id="HCD1930408.1"
                     /translation="MNMFFDWWFATSLRLRQLCWAVWLLVLVTLIFLSSTHQEERDAL
                     IRLRASHHQQWAALYRLVDATPFSEEKTLPFSPLDFQLSGAQLVSWHPSAQGGELALK
                     TLWEAVPSAFTRLAERNVSVSRFSLSVEGDDLLFTLQLETPHEG"
     gene            complement(16644..17183)
                     /gene="hofN"
                     /locus_tag="NBI74_000020"
     CDS             complement(16644..17183)
                     /gene="hofN"
                     /locus_tag="NBI74_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofN"
                     /protein_id="HCD1930409.1"
                     /translation="MNPPINFLPWRQQRRTAFLRFWLLMFVAPLLLAVGITLILRLTS
                     NAEARVNAVLLQAEQQLTRSLQITKPRLLERQQLREQRLQRQRQRQFTRDWQSALEAL
                     AALLPEHAWLTTISWQQGTLEIKGLTTSITALNALETSLRQDASFHLNQRGATQQDAQ
                     GRWQFEYQLTRKVSDEHVL"
     gene            complement(17183..17962)
                     /gene="hofM"
                     /locus_tag="NBI74_000021"
     CDS             complement(17183..17962)
                     /gene="hofM"
                     /locus_tag="NBI74_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofM"
                     /protein_id="HCD1930410.1"
                     /translation="MAFKNWQIGLHLQQQEAVAVAIVRSAKECLLHRWWRLPLENDII
                     KDGRIVDVQRLANTLLPWSRELPQRHHIMLAFPASRTLQRSFPRPSMSLGEREQMAWL
                     SGTMARELDMDPDSLRFDYSEDSLSPAYNVTAAQSKELATLLTLAERLRVHVSAITPD
                     ASALQRFLPFLPSHQQCLAWRDNEQWLWATRYSWGRKLAVGMTSAKELAAALSVDPES
                     VALCGEGGFDPWEAVSVRQPPLPPPGGDFAIALGLALGKVY"
     gene            18082..20634
                     /gene="mrcA"
                     /locus_tag="NBI74_000022"
     CDS             18082..20634
                     /gene="mrcA"
                     /locus_tag="NBI74_000022"
                     /EC_number="2.4.1.129"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase/peptidoglycan
                     DD-transpeptidase MrcA"
                     /protein_id="HCD1930411.1"
                     /translation="MKFVKYFLILAVCCILLGAGSIYGLYRYIEPQLPDVATLKDVRL
                     QIPMQIYSADGELIAQYGEKRRIPVTLDQIPPEMVKAFIATEDSRFYEHHGVDPVGIF
                     RAASVALFSGHASQGASTITQQLARNFFLSPERTLMRKIKEVFLAIRIEQLLTKDEIL
                     ELYLNKIYLGYRAYGVGAAAQVYFGKTVDQLTLNEMAVIAGLPKAPSTFNPLYSMDRA
                     VARRNVVLSRMLDEGYITQQQFDQTRTEAINANYHAPEIAFSAPYLSEMVRQEMYNRY
                     GESAYEDGYRIYTTITRKVQQAAQQAVRNNVLDYDMRHGYRGPANVLWKVGESAWDNN
                     KITDTLKALPTYGPLLPAAVTSANPQEATAMLADGSTVVLSMEGVRWARPYRSDTQQG
                     PTPRKVTDVLQTGQQIWVRQVDDAWWLAQVPEVNSALVSINPQNGAVMALVGGFDFNQ
                     SKFNRATQALRQVGSNIKPFLYTAAMDKGLTLASMLNDVPISRWDAGAGSDWQPKNSP
                     PQYAGPIRLRQGLGQSKNVVMVRAMRAMGVDYAAEYLQRFGFPAQNIVHTESLALGSA
                     SFTPMQVARGYAVMANGGFLVDPWFISKIENDQGGVIFEAKPKVACPECDIPVIYGDT
                     QKSNVLENNDVEDVAISREQQNVSVPMPQLEQANQALVAKTGAQEYAPHVINTPLAFL
                     IKSALNTNIFGEPGWQGTGWRAGRDLQRHDIGGKTGTTNSSKDAWFSGYGPGVVTSVW
                     IGFDDHRRNLGHTTASGAIKDQISGYEGGAKSAQPAWDAYMKAVLEGVPEQPLTPPPG
                     IVTVNIDRSTGQLANGGNSREEYFIEGTQPTQQAVHEVGTTIIDNGEAQELF"
     gene            complement(20800..21360)
                     /gene="nudE"
                     /locus_tag="NBI74_000023"
     CDS             complement(20800..21360)
                     /gene="nudE"
                     /locus_tag="NBI74_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP compounds hydrolase NudE"
                     /protein_id="HCD1930412.1"
                     /translation="MSKSLQKPTILNVETVARSRLFTVESVDLEFSNGVRRVYERMRP
                     TNREAVMIVPIVDDHLILIREYAVGTESYELGFSKGLIDPGESVFEAANRELKEEVGF
                     GANDLTFLKKLSMAPSYFSSKMNIVVAQDLYPESLEGDEPEPLPQVRWPLAHMMDLLE
                     APDFNEARNVSALFLVREWLKGQGRV"
     gene            21680..23815
                     /gene="igaA"
                     /locus_tag="NBI74_000024"
     CDS             21680..23815
                     /gene="igaA"
                     /locus_tag="NBI74_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intracellular growth attenuator protein IgaA"
                     /protein_id="HCD1930413.1"
                     /translation="MSTIVIFLAALLACSLLAGWLIKVRSRRRQLPWTNAFADAQTRK
                     LTPEERSAVENYLESLTQVLQVPGPTGASAAPISLALNAESNNVMMLTHAITRYGIST
                     DDPNKWRYYLDSVEVHLPPFWEQYINDENTVELIHTDSLPLVISLNGHTLQEYMQETR
                     GYALQPVPSTQASIRGEESEQIELLNIRKETHEEYALSRPRGLREALLIVASFLMFFF
                     CLITPDVFVPWLAGGALLLLGAGLWGLFAPPAKSSLREIHCLRGTPRRWGLFGENDQE
                     QINNISLGIIDLVYPAHWQPYIAQDLGQQTDIDIYLDRHVVRQGRYLSLHDEVKNFPL
                     QHWLRSTIIAAGSLLVLFMLLFWIPLDMPLKFTLSWMKGAQTIEATSVKQLADAGVRV
                     GDTLRISGTGMCNIRTSGTWSAKTNSPFLPFDCSQIIWNDARSLPLPESELVNKATAL
                     TEAVNRQLHPKPEDESRVSASLRSAIQKSGMVLLDDFGDIVLKTADLCSAKDDCVRLK
                     NALVNLGNSKDWDALVKRANAGKLDGVNVLLRPVSAESLDNLVATSTTPFITHETARA
                     AQSLNSPAPGGFLIVSDEGSDFVDQPWPSASLYDYPPQEQWNAFQKLAQMLMHTPFNA
                     EGIVTKIFTDANGTQHIGLHPIPDRSGLWRYLSTTLLLLTMLGSAIYNGVQAWRRYQR
                     HRTRMMKIQAYYESCLNPQLITPSESLIE"
     gene            23880..24548
                     /gene="yrfG"
                     /locus_tag="NBI74_000025"
     CDS             23880..24548
                     /gene="yrfG"
                     /locus_tag="NBI74_000025"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP/IMP nucleotidase"
                     /protein_id="HCD1930414.1"
                     /translation="MHINIAWQDVDTVLLDMDGTLLDLAFDNYFWQKLVPETWGAKNG
                     VTPQEAMEYMRQQYHDVQHTLNWYCLDYWSEQLGLDICAMTTEMGPRAVLREDTIPFL
                     EALKASGKQRILLTNAHPHNLAVKLEHTGLDAHLDLLLSTHTFGYPKEDQRLWHAVAE
                     ATGLKAERTLFIDDSEAILDAAAQFGIRYCLGVTNPDSGIAEKQYQRHPSLNDYRRLI
                     PSLM"
     gene            24559..24960
                     /gene="hslR"
                     /locus_tag="NBI74_000026"
     CDS             24559..24960
                     /gene="hslR"
                     /locus_tag="NBI74_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated heat shock protein Hsp15"
                     /protein_id="HCD1930415.1"
                     /translation="MKEKPAVEVRLDKWLWAARFYKTRALAREMIEGGKVHYNGQRSK
                     PSKIVELNATLTLRQGNDERTVIVKAITEQRRPASEAVLLYEETAESVEKREKMALAR
                     KLNALTMPHPDRRPDKKERRDLLRFKHGDSE"
     gene            24985..25863
                     /gene="hslO"
                     /locus_tag="NBI74_000027"
     CDS             24985..25863
                     /gene="hslO"
                     /locus_tag="NBI74_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417860.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HCD1930416.1"
                     /translation="MPQHDQLHRYLFENFAVRGELVTVSETLQQILENHDYPQPVKNV
                     LAELLVATSLLTATLKFDGDITVQLQGDGPMNLAVINGNNNQLMRGVARVQGEIPENA
                     DLKTLVGNGYVVITITPSEGERYQGVVGLEGDTLAACLEDYFMRSEQLPTRLFIRTGD
                     VDGKPAAGGMLLQVMPAQNAQQDDFDHLATLTETIKTEELLTLPANEVLWRLYHEEEV
                     TVYDPQDVEFKCTCSRERCADALKTLPDEEVDSILAEDGEIDMHCDYCGNHYLFNAMD
                     IAEIRNNASPADPQVH"
     gene            complement(25986..27179)
                     /locus_tag="NBI74_000028"
                     /pseudo
     CDS             complement(25986..27179)
                     /locus_tag="NBI74_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            27201..27395
                     /locus_tag="NBI74_000029"
                     /pseudo
     CDS             27201..27395
                     /locus_tag="NBI74_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313209.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(27493..29217)
                     /locus_tag="NBI74_000030"
     CDS             complement(27493..29217)
                     /locus_tag="NBI74_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4153 domain-containing protein"
                     /protein_id="HCD1930417.1"
                     /translation="MDNVELSPATRWGMIATGLLQGLVCYLLIAWLAGKNLSWIVYGV
                     PATVAFSSVLLFSVISFKQNRLWGWLALVFIATLGMSGWLKWQTDGMTPWRAEKALWD
                     FGCYLLLMAMLLLPWIQQSLRIRNDSSRYRYFYQSVWHNVLILLVIFLANGLTWLVLL
                     LWSELFKLVGITFFKTLFFATDWFIYLTLGLVTALAVILARTQSRLIDSIQKLFTLIA
                     TGLLPLVSLLTLMFIITLPFTGLSAISRHISAAGLLLTLAFLQLILMAIVRDPQKASL
                     PWTGPLRCLIKTALLVAPLYVFVAAWALWLRVAQYGWTVDRLQGALAVLVLLVWSLGY
                     FVSIVWRKGQNPLDLQGKVNLAVSLLVLVILVLLNSPVLDSMRISVNSHMARYQSGKN
                     TPDQVTIYMLEQSGRYGRAALESLKSDAEYMKDPKRARDLLMALDGEQHLQEQVSEKV
                     LADNVLIAPGSGKPDATFWSALIQDRYNVMTCIEKDACVLVEQDLNSDGQAERILFAF
                     NDDRVIVYGFDSARKEWDALDMSLLPRKITKEKLLTAAKDGKLGTRPKAWRDLTVDGE
                     TLEINLSK"
     gene            29596..31218
                     /gene="pckA"
                     /locus_tag="NBI74_000031"
     CDS             29596..31218
                     /gene="pckA"
                     /locus_tag="NBI74_000031"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HCD1930418.1"
                     /translation="MRVNNGLTPQELEAYGISDVHDIVYNPSYDLLYQEELDPSLTGY
                     ERGVLTNLGAVAVDTGIFTGRSPKDKYIVRDDTTRDTFWWADKGKGKNDNKPLSPETW
                     QHLKGLVTRQLSGKRLFVVDAFCGANPDTRLSVRFITEVAWQAHFVKNMFIRPSDEEL
                     AGFKPDFIVMNGAKCTNPQWKEQGLNSENFVAFNLTERMQLIGGTWYGGEMKKGMFSM
                     MNYLLPLKGIASMHCSANVGEKGDVAVFFGLSGTGKTTLSTDPKRRLIGDDEHGWDDD
                     GVFNFEGGCYAKTIKLSKEAEPEIYNAIRRDALLENVTVREDGTIDFDDGSKTENTRV
                     SYPIYHIDNIVKPVSKAGHATKVIFLTADAFGVLPPVSRLTADQTQYHFLSGFTAKLA
                     GTERGITEPTPTFSACFGAAFLSLHPTQYAEVLVKRMQAAGAQAYLVNTGWNGTGKRI
                     SIKDTRAIIDAILNGSLDNAETFTLPMFNLAIPTELPGVDTKILDPRNTYASPEQWQE
                     KAETLAKLFIDNFDKYTDTPAGAALVAAGPKL"
     gene            complement(31295..32647)
                     /gene="envZ"
                     /locus_tag="NBI74_000032"
     CDS             complement(31295..32647)
                     /gene="envZ"
                     /locus_tag="NBI74_000032"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     EnvZ"
                     /protein_id="HCD1930419.1"
                     /translation="MRRLRFSPRSSFARTLLLIVTLLFVSLVTTYLVVLNFAILPSLQ
                     QFNKVLAYEVRMLMTDKLQLEDGTQLVVPPAFRREIYRELGISLYSNEAAEEAGLRWA
                     QHYEFLSHQMAQQLGGPTEVRVEVNKSSPVVWLKTWLSPNIWVRVPLTEIHQGDFSPL
                     FRYTLAIMLLAIGGAWLFIRIQNRPLVDLEHAALQVGKGIIPPPLREYGASEVRSVTR
                     AFNHMAAGVKQLADDRTLLMAGVSHDLRTPLTRIRLATEMMSEQDGYLAESINKDIEE
                     CNAIIEQFIDYLRTGQEMPMEMADLNAVLGEVIAAESGYEREIETALYPGSIEVKMHP
                     LSIKRAVANMVVNAARYGNGWIKVSSGTEPNRAWFQVEDDGPGIAPEQRKHLFQPFVR
                     GDSARTISGTGLGLAIVQRIVDNHNGMLELGTSERGGLSIRAWLPVPVTRAQGTTKEG
                     "
     gene            complement(32644..33363)
                     /gene="ompR"
                     /locus_tag="NBI74_000033"
     CDS             complement(32644..33363)
                     /gene="ompR"
                     /locus_tag="NBI74_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014646135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator OmpR"
                     /protein_id="HCD1930420.1"
                     /translation="MQENYKILVVDDDMRLRALLERYLTEQGFQVRSVANAEQMDRLL
                     TRESFHLMVLDLMLPGEDGLSICRRLRSQSNPMPIIMVTAKGEEVDRIVGLEIGADDY
                     IPKPFNPRELLARIRAVLRRQANELPGAPSQEEAVIAFGKFKLNLGTREMFREDEPMP
                     LTSGEFAVLKALVSHPREPLSRDKLMNLARGREYSAMERSIDVQISRLRRMVEEDPAH
                     PRYIQTVWGLGYVFVPDGSKA"
     gene            33591..34067
                     /gene="greB"
                     /locus_tag="NBI74_000034"
     CDS             33591..34067
                     /gene="greB"
                     /locus_tag="NBI74_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312275.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HCD1930421.1"
                     /translation="MKTPLVTREGYEKLKQELNYLWREERPEVTKKVTWAASLGDRSE
                     NADYQYNKKRLREIDRRVRYLTKCMENLKIVDYSPQQEGKVFFGAWVEIENDDGVTHR
                     FRIVGYDEIFGRKDYISIDSPMARALLKKEVGDLAVVNTPAGEASWYVNAIEYVKP"
     gene            34164..36485
                     /locus_tag="NBI74_000035"
     CDS             34164..36485
                     /locus_tag="NBI74_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HCD1930422.1"
                     /translation="MMNDSFCRIIAGEIQARPEQVDAAVRLLDEGNTVPFIARYRKEI
                     TGGLDDTQLRNLETRLSYLRELEERRQAILKSISEQGKLTDDLANAINATLSKTELED
                     LYLPYKPKRRTRGQIAIEAGLEPLADLLWSDPSHTPEVAAAQYIDADKGVADTKAALD
                     GARYILMERFAEDAALLAKVRDYLWKNAHLVSTVVNGKEEEGAKFRDYFDHHEPLSTV
                     PSHRALAMFRGRNEGILQLSLNADPQFEEPPKESYCEQIIMDHLGLRLNNAPADSWRK
                     GVVSWTWRIKVLMHLETELMGTVRERAEDEAINVFARNLHDLLMAAPAGLRATMGLDP
                     GLRTGVKVAVVDATGKLVATDTIYPHTGQAAKAAMTVAALCEKHNVELVAIGNGTASR
                     ETERFYLDVQKQFPKVTAQKVIVSEAGASVYSASELAAQEFPDLDVSLRGAVSIARRL
                     QDPLAELVKIDPKSIGVGQYQHDVSQTQLARKLDAVVEDCVNAVGVDLNTASVPLLTR
                     VAGLTRMMAQNIVAWRDENGQFQNRQQLLKVSRLGPKAFEQCAGFLRINHGDNPLDAS
                     TVHPEAYPVVERILAATQQALKDLMGNSSELRNLKASDFTDEKFGVPTVTDIIKELEK
                     PGRDPRPEFKTAQFADGVETMNDLQPGMILEGAVTNVTNFGAFVDIGVHQDGLVHISS
                     LSNKFVEDPHTVVKAGDIVKVKVLEVDLQRKRIALTMRLDEQPGETNARRGGGNERPQ
                     NNRPAAKPRGREVQPAGNSAMMDALAAAMGKKR"
     gene            36923..37150
                     /gene="feoA"
                     /locus_tag="NBI74_000036"
     CDS             36923..37150
                     /gene="feoA"
                     /locus_tag="NBI74_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter A"
                     /protein_id="HCD1930423.1"
                     /translation="MQYTPDTAWKITGFSREISPAYRQKLLSLGMLPGSSFNVVRVAP
                     LGDPIHIETRRVSLVLRKKDLALLEVEAVSC"
     gene            37167..39488
                     /gene="feoB"
                     /locus_tag="NBI74_000037"
     CDS             37167..39488
                     /gene="feoB"
                     /locus_tag="NBI74_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(2+) transporter permease subunit FeoB"
                     /protein_id="HCD1930424.1"
                     /translation="MKKLTIGLIGNPNSGKTTLFNQLTGSRQRVGNWAGVTVERKEGQ
                     FSTTDHQVTLVDLPGTYSLTTISSQTSLDEQIACHYILSGDADLLINVVDASNLERNL
                     YLTLQLLELGIPCIVALNMLDIAEKQNIRIEIDALSARLGCPVIPLVSTRGRGIEALK
                     LAIDRYKANENVELVHYAQLLLNEADSLAKVMPSDIPLKQRRWLGLQMLEGDIYSRAY
                     AGEASQHLDAALARLRNEMDDPALHIADARYQCIAAICDVVSNTLTAEPSRFTTAVDK
                     IVLNRFLGLPIFLFVMYLMFLLAINIGGALQPLFDVGSVALFVHGIQWIGYTLHFPDW
                     LTIFLAQGLGGGINTVLPLVPQIGMMYLFLSFLEDSGYMARAAFVMDRLMQALGLPGK
                     SFVPLIVGFGCNVPSVMGARTLDAPRERLMTIMMAPFMSCGARLAIFAVFAAAFFGQN
                     GALAVFSLYMLGIVMAVLTGLMLKYTIMRGEATPFVMELPVYHVPHVKSLIIQTWQRL
                     KGFVLRAGKVIIIVSIFLSAFNSFSLSGKIVDNINDSALASVSRVITPVFKPIGVHED
                     NWQATVGLFTGAMAKEVVVGTLNTLYTAENIQDEEFNPAEFNLGEELFSAVDETWQSL
                     KDTFSLSVLMNPIEASKGDGEMGTGAMGVMDQKFGSAAAAYSYLIFVLLYVPCISVMG
                     AIARESSRGWMGFSILWGLNIAYSLATLFYQVASYSQHPTYSLVCILAVILFNIVVIG
                     LLRRARSRVDVELLATRKSVSSCCAASTTGDCH"
     gene            39499..39735
                     /gene="feoC"
                     /locus_tag="NBI74_000038"
     CDS             39499..39735
                     /gene="feoC"
                     /locus_tag="NBI74_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-S]-dependent transcriptional repressor FeoC"
                     /protein_id="HCD1930425.1"
                     /translation="MASLIQVRDLLALRGRMEAAQISQTLNTPQPMINAMLKQLESMG
                     KAVRIQEEPDGCLSGSCKSCPEGKACLHEWWALR"
     gene            40016..40378
                     /locus_tag="NBI74_000039"
                     /pseudo
     CDS             40016..40378
                     /locus_tag="NBI74_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417870.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
     gene            complement(40407..41177)
                     /gene="bioH"
                     /locus_tag="NBI74_000040"
     CDS             complement(40407..41177)
                     /gene="bioH"
                     /locus_tag="NBI74_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pimeloyl-ACP methyl ester esterase BioH"
                     /protein_id="HCD1930426.1"
                     /translation="MNNIWWQTKGQGNVHLVLLHGWGLNAEVWRCIDEELSSHFTLHL
                     VDLPGFGRSRGFGALSLADMAEVVLRQAPDKAIWLGWSLGGLVASQIALTHPERVLAL
                     VTVASSPCFSARDEWPGIKPDVLAGFQQQLSDDFQRTVERFLALQTMGTETARQDARA
                     LKKTVLALPMPEVDVLNGGLEILKTVDLRQPLQNVPMPFLRLYGYLDGLVPRKVVPML
                     DKLWPHSESYIFAKAAHAPFISHPDEFCHLLVALKQRV"
     gene            41215..41898
                     /gene="gntX"
                     /locus_tag="NBI74_000041"
     CDS             41215..41898
                     /gene="gntX"
                     /locus_tag="NBI74_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417872.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein GntX"
                     /protein_id="HCD1930427.1"
                     /translation="MLTVPGLCWLCRMPLALGHWGICSVCSRAARTDKTLCPQCGLPA
                     THSHLPCGRCLQKPLPWQRLLTVADYAPPLSPLIHQLKFSRRSEIASALSRLLLLEVL
                     HARRTTGLQLPDRIISVPLWQRRHWRRGFNQSDLLCQPLSRWLHCQWDSEAVTRTRAT
                     ATQHFLNARLRKHNLKNAFRLELPVQGRHMVIVDDVVTTGSTVAEIAQLLLRNGAATV
                     QVWCLCRTL"
     gene            41957..42532
                     /gene="nfuA"
                     /locus_tag="NBI74_000042"
     CDS             41957..42532
                     /gene="nfuA"
                     /locus_tag="NBI74_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016942932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S biogenesis protein NfuA"
                     /protein_id="HCD1930428.1"
                     /translation="MIRISDAAQAHFAKLLANQEEGTQIRVFVINPGTPNAECGVSYC
                     PPDAVEATDTALKFDLLTAYVDELSAPYLEDAEIDFVTDQLGSQLTLKAPNAKMRKVA
                     DDAPLMERVEYMLQSQINPQLAGHGGRVSLMEITEDGYAILQFGGGCNGCSMVDVTLK
                     EGIEKQLLNEFPELKGVRDLTEHQRGEHSYY"
     gene            42896..44212
                     /gene="gntT"
                     /locus_tag="NBI74_000043"
     CDS             42896..44212
                     /gene="gntT"
                     /locus_tag="NBI74_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HCD1930429.1"
                     /translation="MPLVIVAIGVILLLLLMIRFKMNGFIALVLVALAVGLMQGMPLD
                     KVIGSIKAGVGGTLGSLALIMGFGAMLGKMLADCGGAQRIATTLIAKFGKKHIQWAVV
                     LTGFTVGFALFYEVGFVLMLPLVFTIAASANIPLLYVGVPMAAALSVTHGFLPPHPGP
                     TAIATIFNADMGKTLLYGTILAIPTVILAGPVYARVLKGIDKPIPEGLYSAKTFSEEE
                     MPSFGVSVWTSLVPVVLMAMRAIAEMILPKGHAFLPVAEFLGDPVMATLIAVLIAMFT
                     FGLNRGRSMDQINDTLISSIKIIAMMLLIIGGGGAFKQVLVDSGVDKYIASMMHETNI
                     SPLLMAWSIAAVLRIALGSATVAAITAGGIAAPLIATTGVSPELMVIAVGSGSVIFSH
                     VNDPGFWLFKEYFNLTIGETIKSWSMLETIISVCGLVGCLLLNMVI"
     gene            complement(44323..46407)
                     /gene="malQ"
                     /locus_tag="NBI74_000044"
     CDS             complement(44323..46407)
                     /gene="malQ"
                     /locus_tag="NBI74_000044"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HCD1930430.1"
                     /translation="MESKRLDNAALAAGISPNYINAHGKPQSISAETKRRLLDAMHQR
                     TATKVAVTPVPNVMVYTAGKKMSVAVEGSGEFSWLLTTEEGKQYKGHVTGGKAFNLPT
                     KLPEGYHTLTLTQDDQRAHCRVIVAPKRCYEPQALLNKQKLWGACVQLYTLRSEKNWG
                     IGDFGDLKAMLVDVAKRGGSFIGLNPIHALYLANPESASPYSPSSRRWLNVIYIDVNA
                     VEDFHLSEEAQAWWQLPTTQQTLQQARDADWVDYSTVTTLKMTALRMAWKGFAQRDDE
                     QMTAFRQFVAEQGDSLFWQAAFDALHAQQVKEDEMRWGWPAWPEMYQNVDSPEVRQFC
                     EEHRDDVDFYLWLQWLAYSQFAACWEISQGYEMPIGLYRDLAVGVAEGGAETWCDREL
                     YCLKASVGAPPDILGPLGQNWGLPPMDPHIITARAYEPFIELLRANMQNCGALRIDHV
                     MSMLRLWWIPYGETADQGAYVHYPVDDLLSILALESKRHRCMVIGEDLGTVPVEIVGK
                     LRSSGVYSYKVLYFENDHEKTFRAPKAYPEQSMAVAATHDLPTLRGYWESGDLTLGKT
                     LGLYPDEVVLRGLYQDRELAKQGLLDALHKYGCLPKRAGHKASLMSMTPTLNRGLQRY
                     IADSNSALLGLQPEDWLDMADPVNIPGTSYQYKNWRRKLSATLESMFADDGVNKLLKD
                     LDRRRRAAAKKK"
     gene            complement(46417..48810)
                     /gene="malP"
                     /locus_tag="NBI74_000045"
     CDS             complement(46417..48810)
                     /gene="malP"
                     /locus_tag="NBI74_000045"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin phosphorylase"
                     /protein_id="HCD1930431.1"
                     /translation="MSQPIFNDKQFQEALSRQWQRYGLNSAAEMTPRQWWLAVSEALA
                     EMLRAQPFAKPVANQRHVNYISMEFLIGRLTGNNLLNLGWYQDVQDSLKAYDINLTDL
                     LEEEIDPALGNGGLGRLAACFLDSMVTVGQSATGYGLNYQYGLFRQSFVDGKQVEAPD
                     DWHRGNYPWFRHNEALDVQVGIGGKVTKDGRWEPEFTITGQAWDLPVVGYRNGVAQPL
                     RLWQATHAHPFDLTKFNDGDFLRAEQQGINAEKLTKVLYPNDNHTAGKKLRLMQQYFQ
                     CACSVADILRRHHLAGRKLHELADYEVIQLNDTHPTIAIPELLRVLIDEHQMSWDDAW
                     AITSKTFAYTNHTLMPEALERWDVKLVKGLLPRHMQIINEINTRFKTLVEKTWPGDEK
                     VWAKLAVVHDKQVHMANLCVVGGFAVNGVAALHSDLVVKDLFPEYHQLWPNKFHNVTN
                     GITPRRWIKQCNPALAALLDKSLKKEWANDLDQLINLEKFADDAKFRQQYREIKQANK
                     VRLAEFVKVRTGIEINPQAIFDIQIKRLHEYKRQHLNLLHILALYKEIRENPQSDRVP
                     RVFLFGAKAAPGYYLAKNIIFAINKVADVINNDPLVGDKLKVVFLPDYCVSAAEKLIP
                     AADISEQISTAGKEASGTGNMKLALNGALTVGTLDGANVEIAEKVGEENIFIFGHTVE
                     QVKAILAKGYDPVKWRKKDKVLDAVLKELESGKYSDGDKHAFDQMLHSIGKQGGDPYL
                     VMADFAAYVGAQKQVDVLYRDQEAWTRAAILNTARCGMFSSDRSIRDYQARIWQAKR"
     gene            49433..52138
                     /gene="malT"
                     /locus_tag="NBI74_000046"
     CDS             49433..52138
                     /gene="malT"
                     /locus_tag="NBI74_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MalT"
                     /protein_id="HCD1930432.1"
                     /translation="MLIPSKLSRPVRLDHTVVRERLLAKLSGANNFRLALITSPAGYG
                     KTTLISQWAAGKNDIGWYSLDEGDNQQERFASYLIAAVQQATNGHCAICETMAQKRQY
                     ASLTSLFAQLFIELAEWHSPLYLVIDDYHLITNPVIHESMRFFIRHQPENLTLVVLSR
                     NLPQLGIANLRVRDQLLEIGSQQLAFTHQEAKQFFDCRLSSPIEAAESSRICDDVSGW
                     ATALQLIALSARQNTHSAHKSARRLAGINASHLSDYLVDEVLDNVDLATRHFLLKSAI
                     LRSMNDALITRVTGEENGQMRLEEIERQGLFLQRMDDTGEWFCYHPLFGNFLRQRCQW
                     ELAAELPEIHRAAAESWMAQGFPSEAIHHALAAGDALMLRDILLNHAWSLFNHSELSL
                     LEESLKALPWDSLLENPQLVLLQAWLMQSQHRYGEVNTLLARAEHEIKDIREGTMHAE
                     FNALRAQVAINDGNPDEAERLAKLALEELPPGWFYSRIVATSVLGEVLHCKGELTRSL
                     ALMQQTEQMARQHDVWHYALWSLIQQSEILFAQGFLQTAWETQEKAFQLINEQHLEQL
                     PMHEFLVRIRAQLLWAWARLDEAEASARSGIEVLSSYQPQQQLQCLAMLIQCSLARGD
                     LDNARSQLNRLENLLGNGKYHSDWISNANKVRVIYWQMTSDKAAAANWLRHTAKPEFA
                     NNHFLQGQWRNIARAQILLGEFESAEIVLEELNENARSLRLMSDLNRNLLLLNQLYWQ
                     AGRKSDAQRVLLNALKLANRTGFISHFVIEGEAMAQQLRQLIQLNTLPELEQHRAQRI
                     LREINQHHRHKFAHFDENFVERLLNHPEVPELIRTSPLTQREWQVLGLIYSGYSNEQI
                     AGELEVAATTIKTHIRNLYQKLGVAHRQAAVQHAQKLLKMMGYGV"
     gene            complement(52181..52600)
                     /locus_tag="NBI74_000047"
                     /pseudo
     CDS             complement(52181..52600)
                     /locus_tag="NBI74_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026219.1"
                     /note="catalyzes the conversion of terminal3'-phosphate of
                     RNA to the 2',3'-cyclicphosphodiester; incomplete; partial
                     in the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 3'-terminal phosphate cyclase"
     gene            52530..52967
                     /locus_tag="NBI74_000048"
     CDS             52530..52967
                     /locus_tag="NBI74_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005019152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCD1930433.1"
                     /translation="MFCSCKEKLPASVAISRSATWRGNFRELSASVTRMATFATSGRI
                     TLDTVEDEINRLRYNWQESRPSTLTALLGAEAENIDLFDRMQLEHVIAICRQAKSLSA
                     AGRELFDISRQGKASVNDADRLRKYLARFGLTWEAMQDQHSSS"
     gene            complement(52949..53707)
                     /locus_tag="NBI74_000049"
     CDS             complement(52949..53707)
                     /locus_tag="NBI74_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000815090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family transcriptional regulator"
                     /protein_id="HCD1930434.1"
                     /translation="MKQTQRHNGIIELVKQQGYVSTEELVEHFSVSPQTIRRDLNELA
                     EQNLILRHHGGAALPSSSVNTPWHDRKATQTEEKERIARKVAEQIPNGSTLFIDIGTT
                     PEAVAHALLNHSNLRIVTNNLNVANTLMVKEDFRIILAGGELRSRDGGIIGEATLDFI
                     SQFRLDFGILGISGIDSDGSLLEFDYHEVRTKRAIIENSRHVMLVVDHSKFGRNAMVN
                     MGSISMVDAVYTDTMPPASVMQVLKDHHIQLELC"
     gene            complement(53724..54554)
                     /gene="glpG"
                     /locus_tag="NBI74_000050"
     CDS             complement(53724..54554)
                     /gene="glpG"
                     /locus_tag="NBI74_000050"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease
                     GlpG"
                     /protein_id="HCD1930435.1"
                     /translation="MLMITSFANPRVAQAFVDYMATQGVILTIQQHNQSDVWLADESQ
                     AERVRAELARFLENPADPRYLAASWQSGHTDSGLHYRRYPFFAALRERAGPVTWVMMI
                     ACVVVFIAMQILGDQEVMLWLAWPFDPTLKFEFWRYFTHALMHFSLMHILFNLLWWWY
                     LGGAVEKRLGSGKLIVITLISALLSGYVQQKFSGPWFGGLSGVVYALMGYVWLRGERD
                     PQSGIYLQRGLIIFALIWIVAGWFDLFGMSMANGAHIAGLAVGLAMAFVDSLNARKRK
                     "
     gene            complement(54599..54925)
                     /gene="glpE"
                     /locus_tag="NBI74_000051"
     CDS             complement(54599..54925)
                     /gene="glpE"
                     /locus_tag="NBI74_000051"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001527843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase GlpE"
                     /protein_id="HCD1930436.1"
                     /translation="MDQFECINVADAHQKLQEKEAVLVDIRDPQSFAMGHATQAFHLT
                     NDTLGAFMRDNDFDTPVMVMCYHGNSSKGAAQYLLQQGYDVVYSIDGGFEAWQRQFPA
                     EVAYGV"
     gene            55115..56620
                     /gene="glpD"
                     /locus_tag="NBI74_000052"
     CDS             55115..56620
                     /gene="glpD"
                     /locus_tag="NBI74_000052"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase"
                     /protein_id="HCD1930437.1"
                     /translation="METKDLIVIGGGINGAGIAADAAGRGLSVLMLEAQDLACATSSA
                     SSKLIHGGLRYLEHYEFRLVSEALAEREVLLKMAPHIAFPMRFRLPHRPHLRPAWMIR
                     IGLFMYDHLGKRTSLPGSTGLRFGANSVLKPEIKRGFEYSDCWVDDARLVLANAQMVV
                     RKGGEVLTRTRATSARRENGLWIVEAEDIDTGKKYTWQARGLVNATGPWVKQFFDDGM
                     HLPSPYGIRLIKGSHIVVPRVHTQKQAYILQNEDKRIVFVIPWMDEFSIIGTTDVEYK
                     GDPKAVKIEESEINYLLKVYNTHFKKQLSRDDIVWTYSGVRPLCDDESDSPQAITRDY
                     TLDIHDENGKAPLLSVFGGKLTTYRKLAEHALEKLTPYYQGIGPAWTKESVLPGGAIE
                     GDRDDYAARLRRRYPFLTESLARHYARTYGSNSELLLGNAGAISDLGEDFGHEFYEAE
                     LKYLVDHEWVRRADDALWRRTKQGMWLNADQQSRVSQWLMEYTQQRLSLAS"
     gene            complement(56674..57279)
                     /locus_tag="NBI74_000053"
     CDS             complement(56674..57279)
                     /locus_tag="NBI74_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000589351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1930438.1"
                     /translation="MNKIRLYIYPVIFGFVIVPLLVWPTVIALAVLIFTLTFLAEIIF
                     SFPLLVVRISLQELQLELLVEYALFFSVMGGIGWQFSRRTPPELKNRLHCWLVFSPVY
                     FWLILSNFILYISPEKSALLENIRNFFLTFVWLPLNFSPFWPQPWTDFVGPISAQLGF
                     ALGYYCQWRSKNRSHRKKWGDWVTCLSLAILALGPLFNYLQ"
     gene            complement(57282..58049)
                     /locus_tag="NBI74_000054"
     CDS             complement(57282..58049)
                     /locus_tag="NBI74_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000553742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1930439.1"
                     /translation="MHPTPAMQALIEQIYHIFRRYPAPQQFVVCCEYCLSQQEQKALR
                     NTSLRAIPYSLINAWNSSPGPDPQNSDEVRYFLPCLLEFVAQGQFDNIHEVFSLRRIN
                     LASKENWREDEREILQQFACQYMTDWVSGDEAVELQYKLEMFFRADIALSPLLDAIIS
                     VPGFWSAASLACLLNTYRDGYIRDNQDDIDKAITTQTNTWASNNQSILKERARQAIEN
                     PLKQSEQGTQYQAWEDEWMIDECLCAMYDASSESSGH"
     gene            complement(58152..59660)
                     /locus_tag="NBI74_000055"
     CDS             complement(58152..59660)
                     /locus_tag="NBI74_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PstS family phosphate ABC transporter
                     substrate-binding protein"
                     /protein_id="HCD1930440.1"
                     /translation="MMQSGKWILTSLVMTFFGIPILAQFLAAVVAMLGAGLAAILEVC
                     NLLFTPTIYLLLNVFMLTLGAIIIFFSGRVWAGDSAPEKREIAAWRQCFFLLPALLTL
                     VGWIIALHLADYQFRQMGSGWLANLMLSWQGVLLLSLISGDYWWIVIIPVGAHISFSL
                     GYGWPTRHPLTGTSGLRCRNLLLFLLLLLGFVAGYQAYLYKQLNPGVGVRENIDTWAW
                     RPDKLNNQLTPLRGKPQIQFTQNWPRLDGATAAYPIYASAFYALSVIPEDFHVWDYLD
                     NSRTQEAYNKIVNGDADIIFVAQPSDGQKKRAEKSGVTLLYTPFAREAFVFIVNADNP
                     VNSLTEQQVRDIFSGAITNWRAVGGDDQEIQTWQRPEDSGSQTVMLSQVMKNVRMISP
                     QETEVASVMEGMINVVAEYRNTKNAIGYTFRYYATQMNADKNIKLLAINGIAPTAENI
                     RNDKYPYIVDALMVTRENTTSETQKLVEWFLTPQGQSLVEDVGYVPMDKTLH"
     gene            complement(59785..62232)
                     /gene="glgP"
                     /locus_tag="NBI74_000056"
     CDS             complement(59785..62232)
                     /gene="glgP"
                     /locus_tag="NBI74_000056"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen phosphorylase"
                     /protein_id="HCD1930441.1"
                     /translation="MNAPFTYSSPTLSVEALKHSIAYKLMFTIGKDPVVANKHEWLNA
                     TLFAVRDRLVERWLRSNRAQLSQETRQVYYLSMEFLIGRTLSNAMLSLGIYEDVQGAL
                     EAMGLNLEELIDEENDPGLGNGGLGRLAACFLDSLATLGLPGRGYGIRYDYGMFKQNI
                     VNGSQKESPDYWLEYGNPWEFKRHNTRYKVRFGGRIQQEGKKTRWIETEEILGVAYDQ
                     IIPGYDTDATNTLRLWSAQASSEINLGKFNQGDYFAAVEDKNHSENVSRVLYPDDSTY
                     SGRELRLRQEYFLVSSTIQDILSRHYQLHKTYDNLADKIAIHLNDTHPVLSIPEMMRL
                     LIDEHQFSWDDAFEVCCQVFSYTNHTLMSEALETWPVDMLGKILPRHLQIIFEINDYF
                     LKTLQEQYPNDTDLLGRASIIDESNGRRVRMAWLAVVVSHKVNGVSELHSNLMVQSLF
                     ADFAKIFPGRFTNVTNGVTPRRWLAVANPSLSAVLDEHLGRNWRTDLSLLNDLQQHCD
                     FPMVNHAVHQAKLENKKRLAEYIAQQLNVVVNPKALFDVQIKRIHEYKRQLMNVLHVI
                     TRYNRIKADPDAKWVPRVNIFGGKAASAYYMAKHIIHLINDVAKVINNDPQIGDKLKV
                     VFIPNYSVSLAQLIIPAADLSEQISLAGTEASGTSNMKFALNGALTIGTLDGANVEML
                     DHVGADNIFIFGNTAEEVEELRRQGYKPREYYEKDEELHQVLTQIGSGVFSPEDPGRY
                     RDLVDSLINFGDHYQVLADYRSYVDCQDKVDELYERQEEWTAKAMLNIANMGYFSSDR
                     TIKEYADYIWHIDPVRL"
     gene            complement(62251..63684)
                     /gene="glgA"
                     /locus_tag="NBI74_000057"
     CDS             complement(62251..63684)
                     /gene="glgA"
                     /locus_tag="NBI74_000057"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HCD1930442.1"
                     /translation="MQVLHVCSEMFPLLKTGGLADVIGALPAAQIADGVDARVLLPAF
                     PDIRRGVTDAQVVSRRDTFAGHITLLFGHYNGVGIYLIDAPHLYDRPGSPYHDTNLFA
                     YTDNVLRFALLGWVGAEMASGLDPFWRPDVVHAHDWHAGLAPAYLAARGRPAKSVFTV
                     HNLAYQGMFYAHHMNDIQLPWSFFNIHGLEFNGQISFLKAGLYYADHITAVSPTYARE
                     ITEPQFAYGMEGLLQQRHREGRLSGVLNGVDEKIWSPETDLLLASRYTRDTLEDKAEN
                     KRQLQIAMGLKVDDKVPLFAVVSRLTSQKGLDLVLEALPGLLEQGGQLALLGAGDPVL
                     QEGFLAAAAEYPGQVGVQIGYHEAFSHRIMGGADVILVPSRFEPCGLTQLYGLKYGTL
                     PLVRRTGGLADTVSDCSLENLADGVASGFVFEDSNAWSLLRAIRRAFVLWSRPSLWRF
                     VQRQAMAMDFSWQVAAKSYRELYYRLK"
     gene            complement(63684..64979)
                     /gene="glgC"
                     /locus_tag="NBI74_000058"
     CDS             complement(63684..64979)
                     /gene="glgC"
                     /locus_tag="NBI74_000058"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HCD1930443.1"
                     /translation="MVSLEKNDHLMLARQLPLKSVALILAGGRGTRLKDLTNKRAKPA
                     VHFGGKFRIIDFALSNCINSGIRRMGVITQYQSHTLVQHIQRGWSFFNEEMNEFVDLL
                     PAQQRMKGENWYRGTADAVTQNLDIIRRYKAEYVVILAGDHIYKQDYSRMLIDHVEKG
                     ARCTVACMPVPIEEASAFGVMAVDENDKIIEFVEKPANPPSMPNDPGKSLASMGIYVF
                     DADYLYELLEEDDRDENSSHDFGKDLIPKITEAGLAYAHPFPLSCVQSDPDAEPYWRD
                     VGTLEAYWKANLDLASVVPELDMYDRNWPIRTYNESLPPAKFVQDRSGSHGMTLNSLV
                     SGGCVISGSVVVQSVLFSRVRVNSFCNIDSAVLLPEVWVGRSCRLRRCVIDRACVIPE
                     GMVIGENAEEDARRFYRSEEGIVLVTREMLRKLGHKQER"
     gene            complement(64997..66970)
                     /gene="glgX"
                     /locus_tag="NBI74_000059"
     CDS             complement(64997..66970)
                     /gene="glgX"
                     /locus_tag="NBI74_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HCD1930444.1"
                     /translation="MTQLAIGKPTPLGAHYDGQGVNFTLFSAHAERVELCVFDANGQE
                     HRYDLPGHSGDIWHGYLPDARPGLRYGYRVHGPWQPAEGHRFNPAKLLIDPCARQIDG
                     EFKDNPLLHAGHNEPDYRDNAAIAPKCVVVIDHYDWEDDAPPRTPWGSTIIYEAHVKG
                     LTYLHPEIPVEIRGTYKALGHPVMINYLKQLGITALELLPVAQFASEPRLQRMGLSNY
                     WGYNPVAMFALHPAYACSPETALDEFRDAIKALHKAGIEVILDIVLNHSAELDLDGPL
                     FSLRGIDNRSYYWIREDGDYHNWTGCGNTLNLSHPAVVDYASACLRYWVETCHVDGFR
                     FDLAAVMGRTPEFRQDAPLFTAIQNCPVLSQVKLIAEPWDIAPGGYQVGNFPPLFAEW
                     NDHFRDAARRFWLHYDLPLGAFAGRFAASSDVFKRNDRLPSAAINLVTAHDGFTLRDC
                     VCFNHKHNEANGEENRDGTNNNYSNNHGKEGLGGTLDLVERRRDSIHALLTTLLLSQG
                     TPMLLAGDEHGHSQHGNNNAYCQDNQLTWLDWSQASSGLTAFTAALIHLRKRIPALME
                     NRWWEEGDGNVRWLNRYAQPLSTDEWQNGPKQLQILLSDRFLIAINATLEVTEIVLPA
                     GEWHAIPPFAGEDNPVITAVWQGPAHGLCVFQR"
     gene            complement(66967..69153)
                     /gene="glgB"
                     /locus_tag="NBI74_000060"
     CDS             complement(66967..69153)
                     /gene="glgB"
                     /locus_tag="NBI74_000060"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching enzyme"
                     /protein_id="HCD1930445.1"
                     /translation="MSDRIDRDVINALIAGHFADPFSVLGMHKTTAGLEVRALLPDAT
                     DVWVIEPKTGRKLAKLECLDSRGFFSGVIPRRKNFFRYQLAVVWHGQQNLIDDPYRFG
                     PLIQEMDAWLLSEGTHLRPYETLGAHADTMDGVTGTRFSVWAPNARRVSVVGQFNYWD
                     GRRHPMRLRKESGIWELFIPGAHNGQLYKYEMIDANGNLRLKSDPYAFEAQMRPETAS
                     LICGLPEKVVQTEERKKANQFDAPISIYEVHLGSWRRHTDNNFWLSYRELADQLVPYA
                     KWMGFTHLELLPINEHPFDGSWGYQPTGLYAPTRRFGTRDDFRYFIDAAHAAGLNVIL
                     DWVPGHFPTDDFALAEFDGTNLYEHSDPREGYHQDWNTLIYNYGRREVSNFLVGNALY
                     WIERFGIDALRVDAVASMIYRDYSRKEGEWIPNEFGGRENLEAIEFLRNTNRILGEQV
                     SGAVTMAEESTDFPGVSRPQDMGGLGFWYKWNLGWMHDTLDYMKLDPIYRQYHHDKLT
                     FGMLYNYTENFVLPLSHDEVVHGKKSILDRMPGDAWQKFANLRAYYGWMWAFPGKKLL
                     FMGNEFAQGREWNHDASLDWHLLEGGDNWHHGVQRLVRDLNHTYRHHKAMHELDFDPY
                     GFEWLVVDDKERSVLIFVRRDKEGNEIIVASNFTPVPRHDYRFGINQPGKWREILNTD
                     SMHYHGSNAGNGGAVHSDEIASHGRQHSLSLTLPPLATIWLVREAE"
     gene            complement(69426..70529)
                     /gene="asd"
                     /locus_tag="NBI74_000061"
     CDS             complement(69426..70529)
                     /gene="asd"
                     /locus_tag="NBI74_000061"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HCD1930446.1"
                     /translation="MKNVGFIGWRGMVGSVLMQRMVEERDFDAIRPVFFSTSQLGQAA
                     PSFGGTTGTLQDAFDLEALKALDIIVTCQGGDYTNEIYPKLRESGWQGYWIDAASSLR
                     MKDDAIIILDPVNQDVITDGLNNGIRTFVGGNCTVSLMLMSLGGLFANDLVDWVSVAT
                     YQAASGGGARHMRELLTQMGHLYGHVADELANPSSAILDIERKVTTLTRSGELPVDNF
                     GVPLAGSLIPWIDKQLDNGQSREEWKGQAETNKILNTSSVIPVDGLCVRVGALRCHSQ
                     AFTIKLKKDVSIPTVEELLAAHNPWAKVVPNDREITMRELTPAAVTGTLTTPVGRLRK
                     LNMGPEFLSAFTVGDQLLWGAAEPLRRMLRQLA"
     gene            70721..71314
                     /gene="yhgN"
                     /locus_tag="NBI74_000062"
     CDS             70721..71314
                     /gene="yhgN"
                     /locus_tag="NBI74_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YhgN"
                     /protein_id="HCD1930447.1"
                     /translation="MNEIISATVLLILIMDPLGNLPIFMSVLKHTEPKRRRAIMVREL
                     LIALLVMLVFLFAGEKILAFLSLRAETVSISGGIILFLIAIKMIFPSASGNSSGLPAG
                     EEPFIVPLAIPLVAGPTILATLMLLSHQYPNQMGHLVIALLLAWGGTFVILLQSSLFL
                     RLLGEKGVNALERLMGLILVMMATQMFLDGIRMWMKG"
     gene            complement(71360..72655)
                     /locus_tag="NBI74_000063"
     CDS             complement(71360..72655)
                     /locus_tag="NBI74_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312307.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HCD1930448.1"
                     /translation="MDADSSDSTVTPGRLPDGLSSCPCFYLGKKLMNLSANAQATLLL
                     TSDFSRAAASEYKPLSNSEWGKFALWLKHQRISPAELLVPQPQEKLTGWSDPRISQER
                     ILGLLARGHSLALAVDKWQRAGLWILTRGDADYPVRLKNRLRTDAPPVLFGCGNKALL
                     QAEGMAIVGSRDAPTDDLRYTQQLAAKLAQQGICVISGGARGIDECAMASALEVGGTA
                     VGVLADSLLKTSTLVKWREGLIAGNLVLISPFYPEVRFTVGNAMARNKYIYCLAESAM
                     VVRAGMTGGTITGAMEALKHQWLPMQVKPNQDMQSANSRLVENGASWSAEQAENVTIR
                     LPDVPGLMYDRALRNAQPELFSLHEDDANYAVMPAHTPVDFYQLFVAELAILAKESIS
                     IERLASCTGLTIEQISVWLNRAEEEGRVIRLGEGHYQFR"
     gene            complement(72562..74658)
                     /locus_tag="NBI74_000064"
     CDS             complement(72562..74658)
                     /locus_tag="NBI74_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010349194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RecQ family ATP-dependent DNA helicase"
                     /protein_id="HCD1930449.1"
                     /translation="MEKHGAELLLQRMLSNTSATFREGQWEAIDAVVNQRRKLLVVQR
                     TGWGKSAVYFIASKIFRDRGAGPTIIISPLLALMRNQVAAAERLGITAETLNSTNREE
                     WQRISDKLLQGGVDCLLISPERLANQDFLETVLYPVADRIGLLVVDEAHCISDWGHDF
                     RPDYRRILDILRQLPANTPILGTTATANNRVVEDIRQQLGDIVIQRGTLARESLALDA
                     LVLGEQSSRLAWLATVIPQFSKSGIVYTLTTRDAELVAEWLRKNGISAFAYYSGVTCE
                     GAEDSNTAREYLEQALLANKIKVLVATTALGMGFDKPDLGFVIHYQMPGSIVGYYQQV
                     GRAGRAIDSAVGILLCGGEDRAIHQFFRESAFPAEAQIHEILNVLSENDGLTLRGIEQ
                     RTNLRYGQIEKALKLLVAENPSPVVYTEKLWRRTIVSFSPDHERINHLMNQRKSELAD
                     VESYITTKECKMQFLRRALDEPSAERCGKCSSCLQHPLLSPDIDSDLLHAANLFIKHA
                     DLPLNLNKQVASGAFTQYGFKGNLPAGLQGSTGRILSRWGDSGWGKQVAQEKKTGRFS
                     DELVEACAEMVRQRWNPHPEPTWVCCVPSLRHLDLVPDFARRLAAKLGLPFIDAIEKV
                     VDNPPQKMQQNRFHQCQNLDGAFVITPPLMPGPALLVDDIVDSAWTLTVLTALLRQAG
                     CPTVYPLALASTSVKN"
     gene            complement(74799..76139)
                     /gene="gntU"
                     /locus_tag="NBI74_000065"
     CDS             complement(74799..76139)
                     /gene="gntU"
                     /locus_tag="NBI74_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HCD1930450.1"
                     /translation="MTTLTLVLTAVGSVLLLLFLVMKARMHAFLALMVVSMGAGLFSG
                     MPLDKIAATMEKGMGGTLGFLAVVVALGAMFGKILHETGAVDQIAVKMLKSFGHSRVH
                     YAIGLAGLVCALPLFFEVAIVLLISVAFSMARHTGTNLVKLVIPLFAGVAAAAAFLVP
                     GPAPMLLASQMNADFGWMILIGLCAAIPGMIIAGPLWGNFISRYVELHIPDDISEPHL
                     GEGKMPSFGFSLSLILLPLVLVGLKTIAARFVPEGSTAYEWFEFIGHPFTAILVACLV
                     AIYGLAMRQGMPKDKVMEICGHALQPAGIILLVIGAGGVFKQVLVDSGVGPALGEALT
                     GMGLPIAITCFVLAAAVRIIQGSATVACLTAVGLVMPVIEQLNYSGAQMAALSICIAG
                     GSIVVSHVNDAGFWLFGKFTGATEAETLKTWTMMETILGTVGAIVGMIAFQLLS"
     gene            complement(76143..76670)
                     /gene="gntK"
                     /locus_tag="NBI74_000066"
     CDS             complement(76143..76670)
                     /gene="gntK"
                     /locus_tag="NBI74_000066"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HCD1930451.1"
                     /translation="MSTTNHDHHIYVLMGVSGSGKSAVASEVAHQLHAAFLDGDFLHP
                     RCNIEKMASGEPLNDDDRKPWLQALNDAAFAMQRTNKVSLIVCSALKKHYRDLLREGN
                     PNLSFIYLKGDFDVIESRLKARKGHFFKTQMLVTQFETLQEPGADETDVLVVDIDQPL
                     EGVVASTIEVIKKGK"
     gene            complement(76809..77804)
                     /gene="gntR"
                     /locus_tag="NBI74_000067"
     CDS             complement(76809..77804)
                     /gene="gntR"
                     /locus_tag="NBI74_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate operon transcriptional repressor GntR"
                     /protein_id="HCD1930452.1"
                     /translation="MKKKRPVLQDVADRVGVTKMTVSRFLRNPEQVSVALRGKIAAAL
                     DELGYIPNRAPDILSNATSRAIGVLLPSLTNQVFAEVLRGIESVTDAHGYQTMLAHYG
                     YKPEMEQERLESMLSWNIDGLILTERTHTPRTLKMIEVAGIPVVELMDSKSPCLDIAV
                     GFDNFEAARQMTTAIIARGHRHIAYLGARLDERTIIKQKGYEQAMLDAGLVPYSVMVE
                     QSSSYSSGIELIRQARREYPQLDGVFCTNDDLAVGAAFECQRLGLKVPDDMAIAGFHG
                     HDIGQVMEPRLASVLTPRERMGSIGAERLLARIRGESVTPKMLDLGFTLSPGGSI"
     gene            complement(77897..78592)
                     /gene="yhhW"
                     /locus_tag="NBI74_000068"
     CDS             complement(77897..78592)
                     /gene="yhhW"
                     /locus_tag="NBI74_000068"
                     /EC_number="1.13.11.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004213399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quercetin 2,3-dioxygenase"
                     /protein_id="HCD1930453.1"
                     /translation="MIYLRKANERGHANHGWLDSWHTFSFANYYDPNFMGFSALRVIN
                     DDVIEAGQGFGTHPHKDMEILTYVLEGTVEHQDSMGNKEQVPAGEFQIMSAGTGIRHS
                     EYNPSSTERLHLYQIWIMPEENGITPRYEQRRFDAVQGKQLVLSPDARDGSLKVHQDM
                     ELYRWALVKDEQSVHQIAAERRIWIQVVKGNVTINGVKASTSDGLAIWDEQAISIHAD
                     SDSEILLFDLPPV"
     gene            complement(78715..79752)
                     /locus_tag="NBI74_000069"
     CDS             complement(78715..79752)
                     /locus_tag="NBI74_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HCD1930454.1"
                     /translation="MVINCAFIGFGKSTTRYHLPYVLNRKDSWHVAHIFRRHAKPEEQ
                     APIYSHIHFTSDLDEVLNDPDVKLVVVCTHADSHFEYAKRALEAGKNVLVEKPFTPTI
                     AQAKELFALAKSKGLIVTPYQNRRFDSCFLTAKKAIESGKLGEIVEVESHFDYYRPVA
                     ETKPGLPQDGAFYGLGVHTMDQIISLFGRPDHVAYDIRSLRNKANPDDTFEAQLFYGD
                     LKAIVKTSHLVKIDYPKFIVHGKKGSFIKYGIDQQETSLKANIMPGEPGFAADDSVGV
                     LEYVNDEGVTVREEMKPEMGDYGRVYDALYQTITNGAPNYVKESEVLTNLEILERGFE
                     QASPSAVSLAR"
     gene            80086..80574
                     /gene="yhhY"
                     /locus_tag="NBI74_000070"
     CDS             80086..80574
                     /gene="yhhY"
                     /locus_tag="NBI74_000070"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HCD1930455.1"
                     /translation="MSEIVIRHAETRDYEAIRQIHAQPEVYYNTLQVPHPSEQMWLER
                     LTARPGIKQLVACIDGIVVGHLTIDVQQRPRRSHVADFGICVDSRWKNRGVASALMRE
                     MIEMCDNWLRVDRIELTVFVDNAPAIKVYKKFGFEIEGTGKKYALRNGEYVDAYYMAR
                     VK"
     gene            complement(80635..82368)
                     /gene="ggt"
                     /locus_tag="NBI74_000071"
     CDS             complement(80635..82368)
                     /gene="ggt"
                     /locus_tag="NBI74_000071"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HCD1930456.1"
                     /translation="MIKPTFLRRVAIAALLTGSCFSTVAAPPVSYGVEEDVFHPVRAK
                     QGMVASVDVTATQVGVDILKEGGNAVDAAVAVGYALAVTHPQAGNLGGGGFMLIRSKN
                     GNTTAIDFREMAPAKATRDMFLDDQGNTDSKKSLTSHLASGTPGTVAGFSLALDKYGT
                     MPLNKVVQPAFKLARDGFIVNDALADDLKTYGSEVLPNHENSKAIFWKEGEPLKKGDK
                     LVQANLAKSLEMIAENGPDEFYKGTIAEQIAQEMQKNGGLISKEDLAAYKAVERTPIS
                     GDYRGYQVYSMPPPSSGGIHIVQILNILENFDMQKYGFGSADAMQIMAEAEKYAYADR
                     SEYLGDPDFVKVPWQALTNKAYAKSIADQIDINKAKPSSEIRPGKLAPYESNQTTHYS
                     VVDKDGNAVAVTYTLNTTFGTGIVAGESGILLNNQMDDFSAKPSVPNVYGLVGGDANA
                     VGPNKRPLSSMSPTIVVKDGKTWLVTGSPGGSRIITTVLQMVVNSIDYGMNVAEATNA
                     PRFHHQWLPDELRVEKGFSPDTIKLLEAKGQKVALKEAMGSTQSIMVGPDGELYGASD
                     PRSVDDLTAGY"
     gene            82488..82928
                     /locus_tag="NBI74_000072"
     CDS             82488..82928
                     /locus_tag="NBI74_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2756 family protein"
                     /protein_id="HCD1930457.1"
                     /translation="MKRLLLLTALLPFVGFAQPINTLNNPNQPGYQIPSQQRMQTQMQ
                     TQQIQQKGMLNQQLKTQTQLQQQHLENQINNNSQRVLQSQPGERNPARQQMLPNTNGG
                     MLNSNRNPDSSLNQQHMLPERRNGDMLNQPSTPQPDIPLKTIGP"
     gene            complement(82915..83658)
                     /gene="ugpQ"
                     /locus_tag="NBI74_000073"
     CDS             complement(82915..83658)
                     /gene="ugpQ"
                     /locus_tag="NBI74_000073"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HCD1930458.1"
                     /translation="MSNWPYPRIVAHRGGGKLAPENTLAAIDVGAKYGHKMIEFDAKL
                     SKDGEIFLLHDDNLERTSNGWGVAGELNWQDLLRVDAGSWYSKAFKGEPLPLLSQVAE
                     RCRKHGMMANIEIKPTTDTGPLTGKMVALAARELWTGMTPPLLSSFEIDALEAAQQAA
                     PELPRGLLLDEWRDDWRELTARLGCVSIHLNYKLLDKARVMQLKDAGLRILVYTVNKP
                     QRAAELLRWGVDCICTDAIDVIGPNFTAQ"
     gene            complement(83655..84725)
                     /gene="ugpC"
                     /locus_tag="NBI74_000074"
     CDS             complement(83655..84725)
                     /gene="ugpC"
                     /locus_tag="NBI74_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol 3-phosphate ABC transporter ATP
                     binding protein UgpC"
                     /protein_id="HCD1930459.1"
                     /translation="MAGLKLQAVTKSWDGKTQVIKPLTLDVADGEFIVMVGPSGCGKS
                     TLLRMVAGLERVTTGDIWIDRKRVTEMEPKDRGIAMVFQNYALYPHMSVEENMAWGLK
                     IRGMGKQQIAERVKEAARILELDGLLKRRPRELSGGQRQRVAMGRAIVREPAVFLFDE
                     PLSNLDAKLRVQMRLELQQLHRRLKTTSLYVTHDQVEAMTLAQRVMVMNGGVAEQIGT
                     PVEVYEKPASLFVASFIGSPAMNLLAGRVNNEGTHFELDGGIALPLNGGYRQYVGRKM
                     TLGIRPEHIALSSQAEGGVPLVMDTLEILGADNLAHGHWGEQKLVVRLAHQERPTAGS
                     TLWLHLPENQLHLFDGETGQRV"
     gene            complement(84727..85572)
                     /gene="ugpE"
                     /locus_tag="NBI74_000075"
     CDS             complement(84727..85572)
                     /gene="ugpE"
                     /locus_tag="NBI74_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpE"
                     /protein_id="HCD1930460.1"
                     /translation="MIENRPWLTIFSHTMLILGIAVILFPLYVAFVAATLDKQEVYAA
                     PMTLIPGTHLLENIHNIWVNGVGTNSAPFWRMLLNSFVMAFSITLGKITVSMLSAFAI
                     VWFRFPLRNLFFWMIFITLMLPVEVRIFPTVEVIANLKMLDSYAGLTLPLMASATATF
                     LFRQFFMTLPDELVEAARIDGASPMRFFCDIVFPLSKTNLAALFVITFIYGWNQYLWP
                     LLIITDVDLGTTVAGIKGMIATGEGTTEWNSVMAAMLLTLIPPVVIVLVMQRAFVRGL
                     VDSEK"
     gene            complement(85569..86456)
                     /gene="ugpA"
                     /locus_tag="NBI74_000076"
     CDS             complement(85569..86456)
                     /gene="ugpA"
                     /locus_tag="NBI74_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpA"
                     /protein_id="HCD1930461.1"
                     /translation="MPSSRPVFRSRWLPYLLVAPQLIITVIFFIWPAGEALWYSLQSV
                     DPFGFSSQFVGLDNFVTLFHDSYYLDAFWTTIKFSTFVTVSGLLVSLFFAALVEYIVR
                     GSRFYQTLMLLPYAVAPAVAAVLWIFLFNPGRGLITHFLAEFGYDWNHAQNSGQAMFL
                     VVFASVWKQISYNFLFFYAALQSIPRSLIEAAAIDGAGPIRRFFKIALPLIAPVSFFL
                     LVVNLVYAFFDTFPVIDAATSGGPVQATTTLIYKIYREGFTGLDLASSAAQSVVLMFL
                     VIVLTVVQFRYVESKVRYQ"
     gene            complement(86554..87870)
                     /gene="ugpB"
                     /locus_tag="NBI74_000077"
     CDS             complement(86554..87870)
                     /gene="ugpB"
                     /locus_tag="NBI74_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     substrate-binding protein UgpB"
                     /protein_id="HCD1930462.1"
                     /translation="MKPLRYTASALALGLALMANAQAVTTIPFWHSMEGELGKEVDSL
                     AQRFNAENPDYKIVPTYKGNYEQNLSAGIAAFRTGNAPAILQVYEVGTATMMASKAIK
                     PVYDVFKEAGIQFDESQFVPTVSGYYSDSKTGHLLSQPFNSSTPVLYYNKDAFKKAGL
                     DPEQPPKTWQDLADYAAKLKASGMKCGYASGWQGWIQLENFSAWNGLPFASKNNGFDG
                     TDAVLEFNKPEQVKHIAMLEEMNKKGDFSYVGRKDESTEKFYNGDCAMTTASSGSLAN
                     IREYAKFNYGVGMMPYDADAKDAPQNAIIGGASLWVMQGKDKETYTGVAKFLDFLAKP
                     ENAAEWHQKTGYLPITKAAYDLTREQGFYEKNPGADIATRQMLNKPPLPFTKGLRLGN
                     MPQIRVIVDEELESVWTGKKTPQQALDTAVERGNQLLRRFEKSTKS"
     gene            complement(88267..88980)
                     /gene="livF"
                     /locus_tag="NBI74_000078"
     CDS             complement(88267..88980)
                     /gene="livF"
                     /locus_tag="NBI74_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivF"
                     /protein_id="HCD1930463.1"
                     /translation="MEKVMLSFDKVSAHYGKIQALHEVSLHINQGEIVTLIGANGAGK
                     TTLLGTLCGDPRATSGRIVFDDKDITDWQTAKIMREAVAIVPEGRRVFSRMTVEENLA
                     MGGFFAERDQFQERIKWVYELFPRLHERRVQRAGTMSGGEQQMLAIGRALMSNPRLLL
                     LDEPSLGLAPIIIQQIFDTIEQLREQGMTIFLVEQNANQALKLADRGYVLENGHVVLS
                     DTGDALLANEAVRSAYLGG"
     gene            complement(88982..89749)
                     /gene="livG"
                     /locus_tag="NBI74_000079"
     CDS             complement(88982..89749)
                     /gene="livG"
                     /locus_tag="NBI74_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000084170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivG"
                     /protein_id="HCD1930464.1"
                     /translation="MSQPLLSVNGLMMRFGGLLAVNNVNLELYPQEIVSLIGPNGAGK
                     TTVFNCLTGFYKPTGGTILLRDQHLEGLPGQQIARMGVVRTFQHVRLFREMTVIENLL
                     VAQHQQLKTGLFSGLLKTPSFRRAQSEALDRAATWLERIGLLEHANRQASNLAYGDQR
                     RLEIARCMVTQPEILMLDEPAAGLNPKETKELDELIAELRNHHNTTILLIEHDMKLVM
                     GISDRIYVVNQGTPLANGTPEQIRNNPDVIRAYLGEA"
     gene            complement(89746..91023)
                     /gene="livM"
                     /locus_tag="NBI74_000080"
     CDS             complement(89746..91023)
                     /gene="livM"
                     /locus_tag="NBI74_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     permease LivM"
                     /protein_id="HCD1930465.1"
                     /translation="MKPMHIAMALLSAAMFFVLAGVFMGVQLELDGTKLVVDTASEIR
                     WQWVFIGTAVVFFFQLVRPAFQKGLKSVSGPKFILPAIDGSTVKQKLFLVALLVLAVA
                     WPFMVSRGTVDIATLTMIYIILGLGLNVVVGLSGLLVLGYGGFYAIGAYTFALLNHYY
                     GLGFWTCLPIAGLMAAAAGFLLGFPVLRLRGDYLAIVTLGFGEIVRILLLNNTEITGG
                     PNGISQIPKPTLFGLEFSRTAREGGWDTFSNFFGLKYDPSDRVIFLYLVALLLVVLSL
                     FVINRLLRMPLGRAWEALREDEIACRSLGLSPRRIKLTAFTISAAFAGFAGTLFAARQ
                     GFVSPESFTFAESAFVLAIVVLGGMGSQFAVILAAVLLVVSRELMRDFNEYSMLMLGG
                     LMVLMMIWRPQGLLPMTRPQLKLKNGAAKGEQA"
     gene            complement(91020..91946)
                     /gene="livH"
                     /locus_tag="NBI74_000081"
     CDS             complement(91020..91946)
                     /gene="livH"
                     /locus_tag="NBI74_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010436488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter permease LivH"
                     /protein_id="HCD1930466.1"
                     /translation="MSEQFLYFLQQMFNGVTLGSTYALIAIGYTMVYGIIGMINFAHG
                     EVYMIGSYVSFMIIAALMMLGIDTGWLLVAAGFVGAIVIASAYGWSIERVAYRPVRNS
                     KRLIALISAIGMSIFLQNYVSLTEGSRDVALPSLFNGQWVVGHSENFSASITTMQAVI
                     WIVTFLAMLALTIFIRYSRMGRACRACAEDLKMASLLGINTDRVIALTFVIGAAMAAV
                     AGVLLGQFYGVINPYIGFMAGMKAFTAAVLGGIGSIPGAMIGGLILGIAEALSSAYLS
                     TEYKDVVSFALLILVLLVMPTGILGRPEVEKV"
     gene            complement(91994..93103)
                     /gene="livK"
                     /locus_tag="NBI74_000082"
     CDS             complement(91994..93103)
                     /gene="livK"
                     /locus_tag="NBI74_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter substrate-binding protein LivK"
                     /protein_id="HCD1930467.1"
                     /translation="MKRNAKTIIAGMIALAISHTAMADDIKVAVVGAMSGPIAQWGDM
                     EFNGARQAIKDINAKGGIKGDKLVGVEYDDACDPKQAVAVANKIVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMISPGATNPELTQRGYQYIMRTAGLDSSQGPTAAKYILETVK
                     PQRIAIIHDKQQYGEGLARSVQDGLKAANANVVFFDGITAGEKDFSALIARLKKENID
                     FVYYGGYYPEMGQMLRQARSVGLKTQFMGPEGVGNASLSNIAGDAAEGMLVTMPKRYD
                     QDPTNQGIVDALKADKKDPSGPYVWITYAAVQSLATALERTGSDEPLALVKDLKANGA
                     NTVIGPLNWDEKGDLKGFDFGVFQWHADGSSTAAK"
     gene            93526..93909
                     /gene="panM"
                     /locus_tag="NBI74_000083"
     CDS             93526..93909
                     /gene="panM"
                     /locus_tag="NBI74_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase autocleavage activator
                     PanM"
                     /protein_id="HCD1930468.1"
                     /translation="MKLTIIRLENFSDQDRIDLQKIWPEYSPSSLQVDDNHRIYAARF
                     NERLLAAVRVTLSGTEGALDSLRVREVTRRRGVGQYLLEEVLRNNPGVSCWWMADAGV
                     EDRGVMTAFMRALGFTAQQGGWEKR"
     gene            complement(93906..94433)
                     /locus_tag="NBI74_000084"
     CDS             complement(93906..94433)
                     /locus_tag="NBI74_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCD1930469.1"
                     /translation="MDDLTIEILTDDADYDLQRFDCGEEALNLFLTTHLVRQHRNKIL
                     RAYILCRNTPERQVLGYYTLSGSCFERAALPSKSKQKKIPYKNIPSVTLGRLAIDRSL
                     QGQGWGATLVAHAMKVVWSASLAVGIHGLFVEALNEKAHTFYQSLGFIPLVGENENAL
                     FFPTKSIELLFTPDD"
     gene            complement(94440..94706)
                     /locus_tag="NBI74_000085"
     CDS             complement(94440..94706)
                     /locus_tag="NBI74_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HCD1930470.1"
                     /translation="MSAVKKQRIDLRLTDDDKSMIEEAAAISNQSVSQFMLNSASQRA
                     AEVIEQHRRMILTEESWTRVMDALSNPPSPGEKLKRAAKRLQGM"
     gene            complement(94855..95958)
                     /gene="livJ"
                     /locus_tag="NBI74_000086"
     CDS             complement(94855..95958)
                     /gene="livJ"
                     /locus_tag="NBI74_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     substrate-binding protein LivJ"
                     /protein_id="HCD1930471.1"
                     /translation="MNIKGKALLAGCIALAFSNMALAEDIKVAVVGAMSGPVAQYGDQ
                     EFTGAEQAVADINAKGGIKGNKLQIVKYDDACDPKQAVAVANKVVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMITPAATAPELTARGYQLILRTTGLDSDQGPTAAKYILEKVK
                     PQRIAIVHDKQQYGEGLARAVQDGLKKGNANVVFFDGITAGEKDFSTLVARLKKENID
                     FVYYGGYHPEMGQILRQARAAGLKTQFMGPEGVANVSLSNIAGESAEGLLVTKPKNYD
                     QVPANKPIVDAIKAKKQDPSGAFVWTTYAALQSLQAGLNQSDDPAEIAKYLKANSVDT
                     VMGPLSWDEKGDLKGFEFGVFDWHANGTATDAK"
     gene            complement(96229..97083)
                     /gene="rpoH"
                     /locus_tag="NBI74_000087"
     CDS             complement(96229..97083)
                     /gene="rpoH"
                     /locus_tag="NBI74_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HCD1930472.1"
                     /translation="MTDKMQSLALAPVGNLDSYIRAANAWPMLSADEERALAEKLHYH
                     GDLEAAKTLILSHLRFVVHIARNYAGYGLPQADLIQEGNIGLMKAVRRFNPEVGVRLV
                     SFAVHWIKAEIHEYVLRNWRIVKVATTKAQRKLFFNLRKTKQRLGWFNQDEVEMVARE
                     LGVTSKDVREMESRMAAQDMTFDLSSDDDSDSQPMAPVLYLQDKSSNFADGIEDDNWE
                     EQAANRLTDAMQGLDERSQDIIRARWLDEDNKSTLQELADRYGVSAERVRQLEKNAMK
                     KLRAAIEA"
     gene            complement(97328..98386)
                     /gene="ftsX"
                     /locus_tag="NBI74_000088"
     CDS             complement(97328..98386)
                     /gene="ftsX"
                     /locus_tag="NBI74_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsX"
                     /protein_id="HCD1930473.1"
                     /translation="MNKRDAINHIRQFGGRLDRFRKSVGGSGDGGRNAPKRAKSSPKP
                     VNRKTNVFNEQVRYAFHGALQDLKSKPFATFLTVMVIAISLTLPSVCYMVYKNVNQAA
                     TQYYPSPQITVYLQKTLDDDAAAGVVAQLQAEQGVEKVNYLSREDALGEFRNWSGFGG
                     ALDMLEENPLPAVAVVIPKLDFQGTESLNTLRDRITQINGIDEVRMDDSWFARLAALT
                     GLVGRVSAMIGVLMVAAVFLVIGNSVRLSIFARRDSINVQKLIGATDGFILRPFLYGG
                     ALLGFSGALLSLILSEILVLRLSSAVAEVAQVFGTKFDINGLSFDECLLLLLVCSMIG
                     WVAAWLATVQHLRHFTPE"
     gene            complement(98379..99047)
                     /gene="ftsE"
                     /locus_tag="NBI74_000089"
     CDS             complement(98379..99047)
                     /gene="ftsE"
                     /locus_tag="NBI74_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HCD1930474.1"
                     /translation="MIRFEHVSKAYLGGRQALQGVTFHMQPGEMAFLTGHSGAGKSTL
                     LKLICGIERPSAGKIWFSGHDITRLKNREVPFLRRQIGMIFQDHHLLMDRTVYDNVAI
                     PLIIAGASGDDIRRRVSAALDKVGLLDKAKNFPIQLSGGEQQRVGIARAVVNKPAVLL
                     ADEPTGNLDDALSEGILRLFEEFNRVGVTVLMATHDINLISRRSYRMLTLSDGHLHGG
                     VGHE"
     gene            complement(99050..100546)
                     /gene="ftsY"
                     /locus_tag="NBI74_000090"
     CDS             complement(99050..100546)
                     /gene="ftsY"
                     /locus_tag="NBI74_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HCD1930475.1"
                     /translation="MAKEKKRGFFSWLGFGQKEQTPEKETEVQNEQPVVEEIVQEQEP
                     VKASEQAVEEQPQAHTEAEAETFAADVVEVTEQVAESEKAQPEAEVVAQPEPVVEETP
                     EPVAIEREELPLPEDVNAEAVSPEEWQAEAETVEIVEAAEEEAAKEEITDEEPEAQAL
                     AAEVAEEAVMVVSPAEEDQPVEEIAQEQEKPTKEGFFARLKRSLLKTKENLGSGFISL
                     FRGKKIDDDLFEELEEQLLIADVGVETTRKIITNLTEGASRKQLRDAEALYGLLKEEM
                     GEILAKVDEPLNVEGKTPFVILMVGVNGVGKTTTIGKLARQFEQQGKSVMLAAGDTFR
                     AAAVEQLQVWGLRNNIPVIAQHTGADSASVIFDAIQAAKARNIDVLIADTAGRLQNKS
                     HLMEELKKIVRVMKKLDVEAPHEVMLTIDASTGQNAVSQAKLFHEAVGLTGITLTKLD
                     GTAKGGVIFSVADQFGIPIRYIGVGERIEDLRPFKADDFIEALFARED"
     gene            100696..101292
                     /gene="rsmD"
                     /locus_tag="NBI74_000091"
     CDS             100696..101292
                     /gene="rsmD"
                     /locus_tag="NBI74_000091"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase"
                     /protein_id="HCD1930476.1"
                     /translation="MKKPNHSGSGQIRIIGGQWRGRKLPVPDSPGLRPTTDRVRETLF
                     NWLAPVIVDAQCLDCFAGSGALGLEALSRYAAGATLIEMDRAVSQQLIKNLATLKAGN
                     ARVVNSNAMSFLAQKGTPHNIVFVDPPFRRGLLEETINLLEDNGWLADEALIYVESEV
                     EKGLPTVPANWSLHREKVAGQVAYRLYQREAQGESDAD"
     gene            101282..101551
                     /locus_tag="NBI74_000092"
     CDS             101282..101551
                     /locus_tag="NBI74_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1145 family protein"
                     /protein_id="HCD1930477.1"
                     /translation="MLINIGRLLMLCVWGFLILNLVHPFPRPLNIFVNVALIFTVLMH
                     GMQLALLKSTLPKDGPQMTTAEKVRIFLFGVFELLVWQKKFKVKK"
     gene            complement(101554..101913)
                     /locus_tag="NBI74_000093"
     CDS             complement(101554..101913)
                     /locus_tag="NBI74_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2500 domain-containing protein"
                     /protein_id="HCD1930478.1"
                     /translation="MSKPPLFFIVIIGLIIVAASFRFMQQRREKADNDMAPLQQKLVV
                     VSNKREKPINDRRSRQQEVTPAGTSMRYEASFKPQSGGMEQTFRLDAQQYHALTVGDK
                     GTLSYKGTRFVSFVGEQ"
     gene            102054..102680
                     /locus_tag="NBI74_000094"
     CDS             102054..102680
                     /locus_tag="NBI74_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase"
                     /protein_id="HCD1930479.1"
                     /translation="MLWSFIAVCLSAWLSVDASYRGPTWQRWVFKPLTLLLLLLLAWQ
                     APMFDAISYLVLAGLCASLLGDALTLLPRQRLMYAIGAFFLSHLLYTIYFASQMTLSF
                     FWPLPLVLLVLGALLLAIIWTRLEEYRWPICTFIGMTLVMVWLAGELWFFRPTAPALS
                     AFVGASLLFISNFVWLGSHYRRRFRADNAIAAACYFAGHFLIVRSLYL"
     gene            102754..104952
                     /gene="zntA"
                     /locus_tag="NBI74_000095"
     CDS             102754..104952
                     /gene="zntA"
                     /locus_tag="NBI74_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(II)/Cd(II)/Pb(II) translocating P-type ATPase
                     ZntA"
                     /protein_id="HCD1930480.1"
                     /translation="MSTPDNHGKKAPQFAAFKPLTTVQNANDCCCDGACSSTPTLSEN
                     VSGTRYSWKVSGMDCAACARKVENAVRQLAGVNQVQVLFATEKLVVDADNDIRAQVES
                     AVQKAGYSLRDEQATDEPQESRLKENLPLITLIVMMAISWGLEQFNHPFGQLAFIATT
                     LVGLYPIARQALRLIKSGSYFAIETLMSVAAIGALFIGATAEAAMVLLLFLIGERLEG
                     WAASRARQGVSALMALKPETATRLRNGEREEVAINSLRPGDVIEVAAGGRLPADGKLL
                     SPFASFDESALTGESIPVERATGDKVPAGATSVDRLVTLEVLSEPGASAIDRILKLIE
                     EAEERRAPIERFIDRFSRIYTPAIMAVALLVTLVPPLLFAASWQEWIYKGLTLLLIGC
                     PCALVISTPAAITSGLAAAARRGALIKGGAALEQLGRVTQVAFDKTGTLTVGKPRVTA
                     IHPATGISESELLTLAAAVEQGATHPLAQAIVREAQVAELAIPTAQSQRALVGSGIEA
                     QVNGERVLICAAGKHPADAFAGLINELESAGQTVVLVVRNDDVLGVIALQDTLRADAA
                     TAISELNALGVKGVILTGDNPRAAAAIAGELGLEFKAGLLPEDKVKAVTELNQHAPLA
                     MVGDGINDAPAMKAAAIGIAMGSGTDVALETADAALTHNHLRGLVQMIELARATHANI
                     RQNITIALGLKGVFLVTTLLGMTGLWLAVLADTGATVLVTANALRLLRRK"
     gene            complement(105054..105299)
                     /gene="tusA"
                     /locus_tag="NBI74_000096"
     CDS             complement(105054..105299)
                     /gene="tusA"
                     /locus_tag="NBI74_000096"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA"
                     /protein_id="HCD1930481.1"
                     /translation="MTDLFSSPDHTLDALGLRCPEPVMMVRKTVRNMQPGETLLIIAD
                     DPATTRDIPGFCTFMEHELVAKETDGLPYRYLIRKGG"
     gene            105520..106185
                     /locus_tag="NBI74_000097"
     CDS             105520..106185
                     /locus_tag="NBI74_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine/7-aminomethyl-7-
                     deazaguanine transporter"
                     /protein_id="HCD1930482.1"
                     /translation="MNVFSQTQRYKALFWLSLFHLLVITSSNYLVQLPVSIFGFHTTW
                     GAFSFPFIFLATDLTVRIFGAPLARRIIFAVMIPALLISYVISSLFYMGSWQGFGALA
                     HFNLFVARIATASFMAYALGQILDVHVFNRLRQSRHWWLAPTASTLFGNVSDTLAFFF
                     IAFWRSPDAFMAEHWMEIALVDYCFKVLISIVFFLPMYGVLLNMLLKRLADKSEINAL
                     QAS"
     gene            106258..106815
                     /gene="dcrB"
                     /locus_tag="NBI74_000098"
     CDS             106258..106815
                     /gene="dcrB"
                     /locus_tag="NBI74_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417929.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage sensitivity protein DcrB"
                     /protein_id="HCD1930483.1"
                     /translation="MRNLVKYVGIGLLVMGLAACDDKDTNATAQGSVAESNATGNPVN
                     LLDGKLSFSLPADMTDQSGKLGTQANNMHVWSDATGQKAVIVIMGDDPKEDLAVLAKR
                     LEDQQRSRDPQLQVVTNKAIELKGHKMQQLDSIISAKGQTAYSSVILGNVGNQLLTMQ
                     ITLPADNQQKAQTTAENIINTLVIQ"
     gene            complement(106819..108069)
                     /locus_tag="NBI74_000099"
     CDS             complement(106819..108069)
                     /locus_tag="NBI74_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312349.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCD1930484.1"
                     /translation="MKHCCKNVVILMPEPVAEPALNGLRLNLRIVSIVMFNFASYLTI
                     GLPLAVLPGYVHDVMGFSAFWAGLVISLQYFATLLSRPHAGRYADLLGPKKIVVFGLC
                     GCFMSGLGYLTAGLTASLPVISLLLLCLGRVILGIGQSFAGTGSTLWGVGVVGSLHIG
                     RVISWNGIVTYGAMAMGAPLGVVFYHWGGLQALALIIMGVALVAILLAIPRPTVKASK
                     GKPLPFRAVLGRVWLYGMALALASAGFGVIATFITLFYDAKGWDGAAFALTLFSCAFV
                     GTRLLFPNGINRIGGLNVAMICFSVEIIGLLLVGVATMPWMAKIGVLLAGAGFSLVFP
                     ALGVVAVKAVPQQNQGAALATYTVFMDLSLGVTGPLAGLVMSWAGVPVIYLAAAGLVA
                     IALLLTWRLKKRPPVEIPEAASSS"
     gene            108168..109217
                     /locus_tag="NBI74_000100"
     CDS             108168..109217
                     /locus_tag="NBI74_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HCD1930485.1"
                     /translation="METPQPDKTGMRILLKLASLVVILAGIHAAADIIVQLLLALFFA
                     IVLNPLVTWFIRRGVQRPVAITIVVVVMLIALTALVGVLAASFNEFISMLPKFNKELT
                     RKLFKLQEMLPFLNLHMSPERILQRMDSEKVVTFTTALMTGLSGAMASVLLLVMTVVF
                     MLFEVRHVPYKMRFALNNPQIHIAGLHRALKGVSHYLALKTLLSLWTGVIIWLGLELM
                     GVQFALMWAVLAFLLNYVPNIGAVISAVPPMIQVLLFNGIYECILVGALFLVVHMVIG
                     NILEPRMMGHRLGMSTMVVFLSLLIWGWLLGPVGMLLSVPLTSVCKIWMETTKGGSKL
                     AILLGPGRPKSRLPG"
     gene            109272..109859
                     /gene="acpT"
                     /locus_tag="NBI74_000101"
     CDS             109272..109859
                     /gene="acpT"
                     /locus_tag="NBI74_000101"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase AcpT"
                     /protein_id="HCD1930486.1"
                     /translation="MYRIVLGKVSTLSAAPLPPGLREQAPQGPRRERWLAGRALLSHT
                     LSPLPEIIYGEQGKPAFAPETPLWFNLSHSGDDIALLLSDEGEVGCDIEVIRPRANWR
                     WLANAVFSLGEHAEMDAVHPDQQLEMFWRIWTRKEAIVKQRGGSAWQIVSVDSTYNSS
                     LSVSHCQLENLSLAICTPTPFTLTADSVQWIDSVN"
     gene            109970..111544
                     /gene="nikA"
                     /locus_tag="NBI74_000102"
     CDS             109970..111544
                     /gene="nikA"
                     /locus_tag="NBI74_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter, nickel/metallophore
                     periplasmic binding protein"
                     /protein_id="HCD1930487.1"
                     /translation="MFSTLRRTLFALLACASFIVHAAAPDEITTAWPVNVGPLNPHLY
                     TPNQMFAQSMVYEPLVKYQADGSVIPWLAKSWTHSEDGKTWTFTLRDDVKFSNGEPFD
                     AEAAAENFRAVLDNRQRHAWLELANQIVDVKALSKNELQITLKSAYYPFLQELALPRP
                     FRFIAPSQFKNHETMNGIKAPIGTGPWVLQESKLNQYDVFVRNENYWGEKPAIKKITF
                     NVIPDPTTRAVAFETGDIDLLYGNEGLLPLDTFARFSQNPAYHTQLSQPIETVMLALN
                     TAKAPTNELAVREALNYAVNKKSLIDNALYGTQQVADTLFAPSVPYANLGLKPRQYDP
                     QKAKALLEKAGWTLPAGKDIREKNGQPLRIELSFIGTDALSKSMAEIIQADMRQIGAD
                     VSLIGEEESSIYARQRDGRFGMIFHRTWGAPYDPHAFLSSMRVPSHADFQAQQGLADK
                     PLIDKEIGEVLATHDETQRQALYRDILTRLHDEAVYLPISYISMMVVSKPELGNIPYA
                     PIATEIPFEQIKPVKP"
     gene            111544..112488
                     /gene="nikB"
                     /locus_tag="NBI74_000103"
     CDS             111544..112488
                     /gene="nikB"
                     /locus_tag="NBI74_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikB"
                     /protein_id="HCD1930488.1"
                     /translation="MLRYVLRRFLLLIPMVLAASVIIFLMLRLGTGDPALDYLRLSNL
                     PPTPEMLASTRTMLGLDQPLYVQYGTWLWKALHLDFGISFASQRPVLDDMLNFLPATL
                     ELAGAALVLILLTSVPLGIWAARHRDRLPDFAVRFIAFLGVSMPNFWLAFLLVMAFSV
                     YLQWLPAMGYGGWQHIILPAVSIAFMSLAINARLLRASMLDVAGQRHVTWARLRGLND
                     KQTERRHILRNASLPMITAVGMHIGELIGGTMIIENIFAWPGVGRYAVSAIFNRDYPV
                     IQCFTLMMVVVFVVCNLIVDLLNAALDPRIRRHEGAHA"
     gene            112485..113318
                     /gene="nikC"
                     /locus_tag="NBI74_000104"
     CDS             112485..113318
                     /gene="nikC"
                     /locus_tag="NBI74_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikC"
                     /protein_id="HCD1930489.1"
                     /translation="MNFFLSSRWSVRLALIIIALLALIALTSQWWLPYDPQAIDLPSR
                     LLSPDAQHWLGTDHLGRDIFSRLMAATRVSLGSVMACLLLVLTLGLIIGGSAGLIGGR
                     VDQATMRVADMFMTFPTSILSFFMVGVLGTGLTNVIIAIALSHWAWYARMVRSLVISL
                     RQREFVLASRLSGAGHVRVFVDHLAGAVIPSLLVLATLDIGHMMLHVAGMSFLGLGVT
                     APTAEWGVMINDARQYIWTQPLQMFWPGLALFISVMAFNLVGDALRDHLDPHLVTEHA
                     H"
     gene            113318..114082
                     /gene="nikD"
                     /locus_tag="NBI74_000105"
     CDS             113318..114082
                     /gene="nikD"
                     /locus_tag="NBI74_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikD"
                     /protein_id="HCD1930490.1"
                     /translation="MPQQIELRNIALQAAQPLVHGVSLTLQRGRVLALVGGSGSGKSL
                     TCAATLGILPAGVRQTAGEILADGKPVSPCALRGIKIATIMQNPRSAFNPLHTMHTHA
                     RETCLALGKPADDATLTAAIEAVGLENAARVLKLYPFEMSGGMLQRMMIAMAVLCESP
                     FIIADEPTTDLDVVAQARILDLLESIMQKQAPGMLLVTHDMGVVARLADDVAVMSHGK
                     IVEQGNVETLFNAPKHAVTRSLVSAHLALYGMELAS"
     gene            114079..114885
                     /gene="nikE"
                     /locus_tag="NBI74_000106"
     CDS             114079..114885
                     /gene="nikE"
                     /locus_tag="NBI74_000106"
                     /EC_number="7.2.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikE"
                     /protein_id="HCD1930491.1"
                     /translation="MTLLNVSDLSHHYAHGGFSGKHQHQAVLNNVSLALKSGETVALL
                     GRSGCGKSTLARLLVGLESPSQGNISWRGEPLAKLNRAQRKAFRRDIQMVFQDSISAV
                     NPRKTVREILREPMRHLLSLKKAEQLARASEMLKAVDLDDSVLDKRPPQLSGGQLQRV
                     CLARALAVEPKLLILDEAVSNLDLVLQAGVISLLKKLQQQFGTACLFITHDLRLVERF
                     CQRVMVMDNGQIVETQVVGDKLTFSSDAGRVLQNAVLPAFPVRRRTTEKV"
     gene            114891..115292
                     /gene="nikR"
                     /locus_tag="NBI74_000107"
     CDS             114891..115292
                     /gene="nikR"
                     /locus_tag="NBI74_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-responsive transcriptional regulator
                     NikR"
                     /protein_id="HCD1930492.1"
                     /translation="MQRVTITLDDDLLETLDSLSQRRGYNNRSEAIRDILRSALAQEA
                     TQQHGTQGFAVLSYVYEHEKRDLASRIVSTQHHHHDLSVATLHVHINHDDCLEIAVLK
                     GDMGDVQHFADDVIAQRGVRHGHLQCLPKED"
     gene            115491..116237
                     /locus_tag="NBI74_000108"
     CDS             115491..116237
                     /locus_tag="NBI74_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCD1930493.1"
                     /translation="MKSLSKSSQIPLYQQVVEWIRESIYTGDLVEDDRIPSEYQIMDM
                     LEVSRGTVKKAVAQLVKEGVLIQVQGKGTFVKKENVAYPLGEGLLSFAESLESQKIHF
                     TTEVITSRIEPANRYVAEKLRITPGQDILYLERLRSIGDEKAMLIENRINIELCPGIV
                     EIDFNQHNLFPTIESLSQRKIRYSESRYAARLIGNERGHFLDISEDAPVLHLEQLVFF
                     SRGLPVEFGNVWLKGNKYYLGTVLQRREVS"
     gene            116262..116735
                     /locus_tag="NBI74_000109"
     CDS             116262..116735
                     /locus_tag="NBI74_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HCD1930494.1"
                     /translation="MQGIQFQENFIQRLPAGLSVEQIIRQLAQPLVTAELVVPDFADH
                     VLEREATYPTGLPTEPPCVAIPHTDHKHVRHNAIAVGILPEPVVFADMGGDPDPVPVR
                     VIFLLALGESNKQLNALGWIMEMIQDTPFMCALLTMETTEIHTAILNKMKERGEI"
     gene            116732..117013
                     /locus_tag="NBI74_000110"
     CDS             116732..117013
                     /locus_tag="NBI74_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HCD1930495.1"
                     /translation="MSQTILFVCATGIATSTAVTEKVMEYCKEHGLNVNYSQTNVASL
                     PGNTDGVALVVSTTKVPYELDVPVVNGLPIITGIGEEKVLAQIVSILKK"
     gene            117090..118448
                     /locus_tag="NBI74_000111"
     CDS             117090..118448
                     /locus_tag="NBI74_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HCD1930496.1"
                     /translation="MNDIAHTLYNIVQYILGFGPTVMLPLVLFILALFFKVKPAKALR
                     SSLTVGIGFVGIYAIFDILTSNVGPAAQAMVERTGINLPVVDLGWPPLSAITWGSPIA
                     PFVIPLTILINVAMLALNKTRTVDVDMWNYWHFALAGTLVYYSTGSLFFGLLAAAIAA
                     VVVLKLADWSAPLVQKYFGLEGISLPTLSSVVFFPVGLLVDKIIDHIPGLNRIHIDPE
                     TVQKKFGIFGEPMMVGTILGIFLGVIAGYDFKKVLLLGISIGGVMFILPRMVRILMEG
                     LLPLSEAIKKYLNAKYPDRDDLYIGLDIAVAVGNPAIISTALLLTPISVFIAFVLPGN
                     EVLPLGDLANLAVMASMIALASRGNIFRTVLAAIPVIIADLWIATKIAPFITGMAKDV
                     NFKFTEGSSGQVSSFLDGGNPFRFWLLEIFNGNLIAIALVPVIALVLYGIFRMTRSTV
                     YA"
     gene            118441..119949
                     /locus_tag="NBI74_000112"
     CDS             118441..119949
                     /locus_tag="NBI74_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase"
                     /protein_id="HCD1930497.1"
                     /translation="MPDNSAAIVIDIGTTNCKVTCFSCLDATTLGAHKFVTAKQISPQ
                     GDVDFDIDALWQEVRQAIAQLNAASPLPVRRISIASFGESGVFLDEHGEILTPMLAWY
                     DRRGEEYPATLSEADSAALYDICGLPLHSNYSAFKMRWLLEHYPLRNRRGLRWLHAPE
                     VLLWRLTGEQRTDITLASRTLCLDVRKGEWSAKAAALLHVPCSAFAPLVQPGEHAGWV
                     SESLCKTLGFSQPVSVTLAGHDHMVGARALQMMPGDILNSTGTTEGILQLDTQPTLDE
                     QAKRDKLANGCYSLANQFTLFASLPVGGFALEWLRNTFRLTDEEIAASLNRGYADYLA
                     GNWSLDDIPVFIPHLRGSGSPYKNRHTRGLFYGLGDTLNIDMLISSVSLGLTMEFANC
                     FACFNVPGTSVLKVIGPAAHNPLWLQLKADILQRPVEAIAFSEAVSVGALLTAAPDIP
                     PPQVTIAQRLLPNRARYHQLQRYQHKWESWYQLKLQQEGVMPLHHREEHYVD"
     gene            119939..120208
                     /locus_tag="NBI74_000113"
     CDS             119939..120208
                     /locus_tag="NBI74_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HCD1930498.1"
                     /translation="MLTKTVEVRNSTGLHARPAACLAKAAKKYSCKVTLHYEGNDINA
                     TSMMNIMRAGIKGGKTVEIRCDGDDENEAIQTLTALFRDRFGEAE"
     gene            120240..121091
                     /locus_tag="NBI74_000114"
     CDS             120240..121091
                     /locus_tag="NBI74_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase"
                     /protein_id="HCD1930499.1"
                     /translation="MPLVNGRILLDRIQEKRVLAGAFNTTNLETTISILNAIERSGLP
                     NFIQIAPTNAQLSGYDYIYEIVKRHADKMDVPVSLHLDHGKTQEDVKQAVRAGFTSVM
                     IDGAAFSFEENIAFTQEAVDFCKSYGVPVEAELGAILGKEDDHVSEADCKTEPEKVKT
                     FVERTGCDMLAVSIGNVHGLDDIPRIDIPLLKRIAEVSPVPLVIHGGSGIAPEILRSF
                     VNYRVAKVNIASDLRKAFITAVGKAYVNNHNEANLARVMASAKNAVEEDVYSKILMMN
                     EGHRSVK"
     gene            complement(121200..122324)
                     /locus_tag="NBI74_000115"
     CDS             complement(121200..122324)
                     /locus_tag="NBI74_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCD1930500.1"
                     /translation="MRHLRNIFNLGIKELRSLLGDKAMLTLIVFSFTVSVYSSATVTP
                     GSLNLAPIAIADMDQSQLSNRIVNSFYRPWFLPPEMITADEMDAGLDAGRYTFAINIP
                     PNFQRDVLAGRQPDIQVNVDATRMSQAFTGNGYIQNIINGEVNSFVARYRDNSEPLVS
                     LETRMRFNPNLDPAWFGGVMAIINNITMLAIVLTGSALIREREHGTVEHLLVMPITPF
                     EIMMAKVWSMGLVVLVVSGLSLVLMVKGVLGVPIEGSIPLFMLGVALSLFATTSIGIF
                     MGTIARSMPQLGLLVILVLLPLQMLSGGSTPRESMPQMVQDIMLTMPTTHFVSLAQAI
                     LYRGAGFEIVWPQFLTLIAIGGAFFTIALLRFRKTIGTMA"
     gene            complement(122324..125059)
                     /gene="rbbA"
                     /locus_tag="NBI74_000116"
     CDS             complement(122324..125059)
                     /gene="rbbA"
                     /locus_tag="NBI74_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated ATPase/putative transporter
                     RbbA"
                     /protein_id="HCD1930501.1"
                     /translation="MMHLELVPVPPVAQLAGVSQHYGKTVALNNITLDIPARCMVGLI
                     GPDGVGKSSLLSLISGARVIEQGGVMVLGGDMRDPKHRRDVCPRIAWMPQGLGKNLYH
                     TLSVYENVDFFARLFGHDKAEREVRINELLASTGLAPFRDRPAGKLSGGMKQKLGLCC
                     ALIHDPELLILDEPTTGVDPLSRAQFWDLIDSIRQRQSNMSVLVATAYMEEAERFDWL
                     VAMNAGEVLATGSAEELRQQTQSATLEEAFINLLPQAQRQAHQAVVIPPYQPENAEIA
                     IEARDLTMRFGSFVAVDHVNFRIPRGEIFGFLGSNGCGKSTTMKMLTGLLPASEGEAW
                     LFGQPVDPKDIDTRRRVGYMSQAFSLYNELTVRQNLELHARLFHIPEAEIPARVAEMS
                     ERFKLNDVEDVLPESLPLGIRQRLSLAVAVIHRPEMLILDEPTSGVDPVARDMFWQLM
                     VDLSRQDKVTIFISTHFMNEAQRCDRISLMHAGKVLASGTPPELVEKRGAASLEEAFI
                     AYLQEAAGQSNEAEAPPVVHDTTHAPRQGFSLRRLFSYSRREALELRRDPVRSTLALM
                     GTVILMLIMGYGISMDVENLRFAVLDRDQTVSSQAWTLNLSGSRYFIEQPPLTSYDEL
                     DRRMRAGDITVAIEIPPNFGRDIARGTPVELGVWIDGAMPSRAETVKGYVQAMHQSWL
                     QDVASRQSTPASQSGLMNIETRYRYNPDVKSLPAIVPAVIPLLLMMIPSMLSALSVVR
                     EKELGSIINLYVTPTTRSEFLLGKQLPYIALGMLNFFLLCALSVFVFGVPHKGSFLTL
                     TLAALLYIIIATGMGLLISTFMKSQIAAIFGTAIITLIPATQFSGMIDPVASLEGPGR
                     WIGEVYPTSHFLTIARGTFSKALDLTDLWQLFIPLLIAIPLVMGLSILLLKKQEG"
     gene            complement(125056..126123)
                     /locus_tag="NBI74_000117"
     CDS             complement(125056..126123)
                     /locus_tag="NBI74_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HCD1930502.1"
                     /translation="MDKSKRHLAWWGVGALAVAAVVAWWLLRPAGVPEGFAVSNGRIE
                     ATEVDIASKIAGRIDTILVKEGQFVREGEVLAKMDTRVLQEQRLEAIAQIKEAQSAVA
                     AAQALLEQRQSETRAAQSLVNQRQAELDSVAKRHTRSRSLARRGAISAQQLDDDRAAA
                     ESARAALESAKAQVSASKAAIEAARTNIIQAQTRVEAAQATERRIAADIDDSELKAPR
                     DGRVQYRVAEPGEVLAAGGRVLNMVDLSDVYMTFFLPTEQAGTLKLGGEARLILDAAP
                     DLRIPATISFVASVAQFTPKTVETSDERLKLMFRVKARIPPELLQQHLEYVKTGLPGV
                     AWVRVNEELPWPDDLVVRLPQ"
BASE COUNT        30222 a        34579 c        31947 g        29794 t
ORIGIN      
        1 aacaaattta ttaaagttgc actggtaggc gcagtactgg ctacgttaac tgcatgtact
       61 ggtcatattg aaaaccgtga taagaactgc tcttacgact acctgctgca cccggcaatt
      121 tctatttcta aaatcattgg cggttgcggt ccgactgcac agtaagtctc tgacagaaat
      181 cggctaacgc cggtttaatt gcctgacgcc ctcggagtta tccgagggcg tatttgtttc
      241 tgcattcttt tcgaaaatat cgtttacaac gattaaatgc tggcaatact ttcacgcaag
      301 atccgcaata tcgtctcttc accgccgcga tttgggttaa tgcgaatcgc gcaatgttct
      361 aactgcggat tcgcctggcg aaacgttccg gaaagtcgat aaaagagcgg cgggatttca
      421 tatttcgact ctgcaccaac cgggtaaggc aaggcaccga gtttctgcgc ctcttccagc
      481 actctggcgg caatcggctg atgaaactcc acaatcaata ccttcgactg tgcattggca
      541 atgacggcgc ttttcacttc cgccaccgcg ccgccgttaa gcaaagccag caaccgttca
      601 gatacccccg cctgcaccgc gtgcatcact ggcgcgagca ccagaccacg caacacctct
      661 aatgcctgcg ccccctgaat ctgactgccg ccggaataga gcgtggcgcg aatccggcta
      721 ataacatcgg catcgcctac caccgcacca acgccctctg gcccaaatag cttgaagcag
      781 gaaaatgtcg acacattcgc cccgcattcg cagccgattc gcgctacctt catcaccgca
      841 tagttgtcat cggttaacgc tggaacacct gccgcgcgca acgttgccag cacgtctgcc
      901 agcacatagc ggtcctgcgg ctgctggcgc gtatgctgca ccagcgcggc atcaggttgt
      961 tgctcgtcaa caacctgctt cagtgccgac agatcattga aatcagccgt aatgagcgtt
     1021 agccccatct gctcaataat gacgcgcgtc gttggataaa ccggcgcgtc atgcaccaga
     1081 agacgctgcc ccgatttgag caaagctgcc agcgccgcac ggatcgcgcc agtacccgcg
     1141 ccctgtacca gcgccgcggc ctgtgcgtga aacgcgtcag ccagcacctg ttccacacgc
     1201 tgggtaatgc ggggctgatt tagccccggc gttaagccca aatcaccgca ggtaagaaac
     1261 tcgctgccag gaaaatgacg gcaaatcgtg tccaccagcg caaactgctt ttgctgcgcc
     1321 tcaataagcg tcaggctttg cagaggaaac gtcttcatag gtgccttcct taagccggaa
     1381 cgaacaggcc aagccagtaa agaacattca gcaggatgcc ggtgatcatt actgccacga
     1441 ccggtgccgc cattttctgt accggacggc ccagggattc gttgaggaag taaatggcaa
     1501 ccgcgataga gaatccggta tagcccgcca ttttgattgc tgcaaagatc gaaccgacca
     1561 gcaacgccac ttccatcagc atattcatgg cgttacggat gttatccgac gcattacgta
     1621 ccgacgggta gcgccccaac catttgccga tagaacgaag cagcaagact tccgccgaaa
     1681 taaccactgc gcctaatacc gctgcaacca tcggattcgg cgagagatag cccaccgcat
     1741 aaacaaaggt aaagcccgca actgcataca cgccggtggc taacgccgtg gtggcaatca
     1801 acggcacaaa acccagcccg cgcataaatt ctgccagtgc cgcctggttg attagtgttt
     1861 gcgattgttc cggcgttacg cctgcggagt acgctttctc cagtgtgaag atcgacactt
     1921 cactaccggc gaaaatcttc atgctggcaa cggcagcaat caatgcaccc acgatggcga
     1981 tatacggtaa gtttttgata atccgtgatg tacgctcttc aaacaccgac ataccgctgg
     2041 catcgatatc attttcatca cgatgacgca ggtcatgagt tattgcgatc cccagcagca
     2101 tcaccatgcc aataaagatt tcgatggatt cagggttaag atgtgggaaa tagcgtacta
     2161 ccaccacacg agtcatcagt accaccacgg cggcgatcag actttgcttc cagccaaact
     2221 ggtagaaaat cgccaccaac gggaacagtg caaaagctga aaccaccggc gagcttaatt
     2281 cccccaggct acctaataca tccaccggta gcgcggtcag cagctggttt actggcaaca
     2341 gacaggtgag gatcaatatt ccccagatag caccaagacc aaacgccatc aggctgttta
     2401 tcgccagtac gccgaggata tcggtaggaa gaaagagtaa ccatgcgttg agcagcccgg
     2461 ttttcagggt aaaagagatc cccaccgagg ccacaaaacc aatgctcaga ccaaaagcga
     2521 tgctccccgc ctcgcgacgg ttcatatagc cttcaatcag ttgtggcagg atcgggcgga
     2581 taccgtcatg aaaaacggcc gccgagcgat gcgccagaag cgatgtcata cccgtcaggc
     2641 acgccaccac gataatctga atatacagat ccatagtcgt gccttttatt ttagatggtt
     2701 aatcagcatc ggaatcgcgt gttcaacgtg ctcaacggaa aggccaaacg ccacttttcc
     2761 ttcagcgatc attttggcga tatgttcgtc tttcgcttta atgcccggct tggcaatggt
     2821 gcaacttttg ttatagccaa tcaccgcgat agcaatcgac aaggctgcgc ccgcaccggt
     2881 attacacgcg ccgatgtagt aatccagttg tccggatttc actttcattg ccgcttccat
     2941 gtcgttgtga atgaaaactt caaaacagcc aggagccgtc gcttcaatgg tttttttaat
     3001 ctgctcacgc tgtaagcctg caacgccaat ctttttcatg agtctgtcct tattggaaaa
     3061 attgagaggg attttcacgt aacatcacat ccacatcggc ctgactgaat cctgactggc
     3121 gcaattgcgg aataaaggtg gtcaataaaa agtcatagcc ataaccaccg ttggctttta
     3181 aatgagagcg ccgcgtaata tccatcgaca gcatgacgcg gttcagcaac ccacggtcac
     3241 gtagcgcatg gagcatcgca atgcgctttt cgtccgggta gtaactgttc ttgccgatgg
     3301 tgtcgaactg cacatacgcg ccgagatcga tcatcttcaa aatgttgtcg agattgtctt
     3361 tcagatcgca gtggccgacg gtgacgcgcg aaagatcaac tccgtgggct tgcaacagag
     3421 ccagttgctc cagccccatc gtgctgaacg acgtatgcgt ggagatcggg cgtccggtct
     3481 ggttatgcgc cagcgcggcg gcaataaaca ccttctcttc cagcgacgta atcttccctt
     3541 cgctggagcc gatctccgcg ataattccgg ctttcaggtc agtgccatcg ataccctgct
     3601 caatttcatc gaccatctcc tgcgccagtt cctgcacgct gcgggtcgcc acatgttccg
     3661 ggaaaaacgc gtcctggtaa taaccggtac agcccaccac gttgatcccg gtttcgcgca
     3721 ttacatcgag cataaattgc gcattgcgcc ccatgtaacg gttggtcatc tcaatcacat
     3781 tacgcacgcc ccgggccatc aggtcgttca tctcctggca aatgaacgca tactgatcaa
     3841 ggcggcagtc cacgttgttt ttaaagccgg aaagatcaat atgcagatgc tcatgggcga
     3901 gggtgtagcc cgtcggatca aaactcatag ggtgtctcct gcaaaccgga aggaggaaag
     3961 aaaagagcga ccattttgtg gcggtggcgc acggaaaaat tcacacacgg tagcccccac
     4021 atcagaaagc gtggtgcgca caccgagctg cgtagcgacc agtccttgct gataaaccag
     4081 cactggcacc acttcgcggg tatggtggct gtgaccgatg gtcggatcgt taccgtgatc
     4141 cgccatcacg accaggcaat catctggctg catcgcctca acaagccggg caaggttacg
     4201 gtcaacgacc tgcaaacgtt cggcataacg tgcgacgtct tctgcatgac cagcgaggtc
     4261 ggtttcctgg atattggtac aaataaacgc cgtcggatag gtgttaaatt cgttgagggt
     4321 gatatccata atccgctggc tatccaccag gttttgccag ctcacgccat aaggattgct
     4381 gacgatatct gccaccttac ccaccagcac ggttggcacg cccgcttcat acagtttttg
     4441 cggcacctgc actttttcat cgacaccgta gcccatatgt acgacctgga aaccgttgtc
     4501 ataggcgcca gaacgcggcg cattgatgcc aataaagcgc ccttctttgc tttctgcggc
     4561 atcgaggatg cactggctgt cggttaacag gccaccaaat gtaatgaccc gaccgacctg
     4621 tacctgctca cgcacgatac gacccatttt gattgcgtcg tcaaaagaga tcacagagag
     4681 attggcggtg atgttataga cctgacctaa atccgcctcg agattatcgc caatcgcaac
     4741 cgcctgattg acccacagaa attgcagatc atcgccacga cgctcaacct gccagccagc
     4801 ggaaactaat gcctgctcaa cacggtcaat cacatcacga aagggcatcc gcagcggcgg
     4861 taatgggcgc gtacctaaaa tttcctgatg ccccataaag gtatcgccac cttcatgttg
     4921 cagctctgcc acgccccagg ttgcggagtc tgacggctgc atatcgccag gcgcataacc
     4981 caatgcgttg attagcccca gcgtctccag cgttggtagc tgcaaatgcg gcaactggct
     5041 caggatgtga ccacaggtat tcgccccggc atcctgcgga cgcaccaacg tgacatcttt
     5101 cattgcccct acgccaaagc tatcaatcac taacaccaca aatcgcgcca tttacgcctc
     5161 cagagggttg ccgaggctgt cataacgacc aactatttct ggttcgccat ggtgaatacc
     5221 ggacaccaac accacatcgc tacgggtgat aaaaatctgc gtgcgaaaac agagcaccac
     5281 tgcactgctt accggatgct cgcctgccag cggcagggtg tagtcgatac tgctgtcatc
     5341 cacggcgttg agatatgttt cagtaattct ttgattttct ggcgtaaaca ccagcgcatg
     5401 ttgcgcgtga ccacgacggt agtaaccgcc gccgtagcag tagctgtcgc cacggaaatg
     5461 atgggagatt tcacttaacc agagcatcgc gatacgttca ggctgatcgc cccgctggtt
     5521 tgccggaata gtgcccgtca gcgcatgacc gggttcggta tgagtcacac cgtattccac
     5581 cagcaatggc agcgacgtgc agctggtcgc tgaaggcgcg ttcagttgct caatcgcaat
     5641 accagatttc gccagttgat cccgtgcttg taccagcgtg tgaagattcg gtgtcggcaa
     5701 aactttcccg gcagcctcat cccaaagcag gcaagggaaa tgggtaagtc ccgccaaatg
     5761 tagccccggc agattctgga tttcagcaac cacctcgtgc agcgaatgct gtacaaaacc
     5821 gctctcctgc cccggataaa gaaaatcatc gtcgctataa actttgagca gcacagactg
     5881 cacacgtccg gccttcaccg ctgccgcaga aatttcccgc gctttgtcga gggtaaacac
     5941 agtgatgacg tcggttccct gttcaacggc gtcagaaacc tgatgacaag ggatttgtac
     6001 cagatgcccc tgatgcgcta ccggcaaacc ggcgcggcgc atgactcgcg cctctttgta
     6061 atccaccgcc acaatgccgc tgtagcccag agccagcagt ttttctgcca gccacggatt
     6121 acgaccgaat tgtttggtca tcagatacag ttcaatgccg taaagccgcg ccgtctcaat
     6181 cagccgcttg ccgttttcca gaacttgatc cacgtcgatc acccaactgt ccggggcgat
     6241 cttgccctgc tgccacaaac ttagtgcggc ggaaatcagc gccgggttct ggcgtttcaa
     6301 tgcttctaca aacatcttgt tgcctcgttt ctgttccttg aattaaatat tcattttttt
     6361 gaatatttag gttgtacgac gtggatttat cgggagcttc aaacctcttc gttagcagcc
     6421 atccataatc cataaaggtt ggccagcaaa tagccttctt cacacgggtt aacttccagt
     6481 gcaaattcct tcaacaatac ctgatgcaat tgcacaacgg cttgccagtg gctggattgc
     6541 gccagttccg ccagcaattc gtcatccagc ggctctattt cttcaccacg gcgactgcgc
     6601 atcagtgcac tcgccatatg tgtcatcgcc atcgttcctt gctcactgcg caccggtaga
     6661 tggcactctg tttccagtac attgacgacc tgcatcatgc ctttgcagat gtccttatca
     6721 ataacgcctg catcgcaaag caggttgagt ctggtttcca tatcagatgc ctttcgttta
     6781 ataactgggt tcctgttccc cactgactac tcgctgttga tgagcaagca gggcatgttt
     6841 atcaacaact gcacgcagca gttgttgcat tgggtaatca tcgactcgcg tcaggaccac
     6901 ggcttcggtg gcctgtaaaa atcgcggatc gtctgtgagc ggcgtcgctt ctaatcccag
     6961 catggtcagt tcgttttccg ccactacgtt ccagataacc gcgtcaacat cgcctttaac
     7021 aatgcgttgt aaactctcgt gataagagag atcgactcgt tccacatcac tatcgccaaa
     7081 aaagacatcg gtcatgattt tctgatccgc cgaacggtta tccagcccca cgcgcttcac
     7141 gtttgcggac tcgcctttac ggcaaatcaa ctggtgctcg ccaacgtagg tatgcggtcc
     7201 caactccagc gcgaggcata atcctttttg cgtgagataa ctttccgccg ccagtcgcga
     7261 aaccaccgcc atgtcataca cgccgttaag taaacactcc acgcgaatat ccgcgccgcg
     7321 catatgcgca tagtaaaaag gaatgccatc aaactgggct ttcaatccgc tcgccaggcc
     7381 ttcgtacaaa cgggtatagg gcaagggcat cgcacatacc acgttgttga tatccacatg
     7441 agtcagcaat gctttgttat ccatctcaac cagataactg ccattgcgcc cacggcgttc
     7501 aatccgtatc gctccgcttg attccagcgt tttcaacgcg gcctgcgtca ggccaaccga
     7561 tgaacggcat tcagttgcca gctcatctat ggttttcagt cgattaccgc acttttcacc
     7621 caacaaataa cgggccagtg tcgtgataac gacgccttct tttttgataa acgttcgacg
     7681 cataatatat tttcaataaa atgaatgttt atatcttcag gaatttgaag ataaggcgca
     7741 acagcgagat gtggaaacgg cgaggcgctt cacatttttt cggattattg caaatgagga
     7801 taaaaaaacc gggtttcccc ggtttttttg gcattaatgt taaattacgg cttcgccaca
     7861 aaaccaatcg cttcgtacac cgcttttagc gtacgggaag cgtgtgcgct ggctttttct
     7921 gcgccgtctt tcatcacctg ttgcaggaag gcttcatcgt tgcggaaacg gtggtaacgt
     7981 tcctgcaatt cagtcagcat accggaaacg gcatcagcca cttcaccttt cagatgacca
     8041 tacatcttgc cttcgaactg tttttccagt tccgggatgc tctggcccgt taccgcagaa
     8101 aggatatcca gcaggttgga aacgcccgct ttgttctgca catcgtagcg aactactggc
     8161 ggctcgtcgg agtcagtgac cgcgcgtttg attttcttca ctaccgattt cggatcttcc
     8221 agcaggccga taacgttatt gcggttatcg tcagacttgg acattttctt ggtcggctcc
     8281 agcagcgaca ttacgcgcgc gccagatttc ggaataaacg gctctggcac cttaaagata
     8341 tcgccgtaca gcgcgttgaa acgctgggcg atatcgcggc tcagttccag gtgctgtttc
     8401 tggtcttcac ccaccggtac cagatttgtt tgatacagca ggatgtccgc tgccatcagc
     8461 accggatagt caaacagacc agcgttgatg ttctcggcat aacgcgcaga tttatcttta
     8521 aactgcgtca tgcggctcag ttcgccgaag taggtatagc agttcaatgc ccagcctaac
     8581 tgcgcatgtt ccggcacgtg ggactgaaca aaaatggtgc ttttctcagg atcgatacca
     8641 caagccagat acaacgccag cgtatccagc gtcgctttac gcagcttctg tgcatcctgg
     8701 cgcacggtga tcgcatgttg gtcaacgata cagtaaatgc aatggtagtc atcctgcatg
     8761 tttacccact gacgcagcgc acccatgtag ttaccaatgg tcaattcacc tgagggctgt
     8821 gcgccactaa aaacgatggg cttagtcatt tttcgattcc tgattttcgc tatgcggaag
     8881 ccctaatgcg ggcagaaggt catttataga ctgataaatt acatcaggct ggctgagatc
     8941 gatagcctcg ccgtagttat atccgtaggt taagccaact gatgggcaac ctgccgcttt
     9001 tgctgcctga atatcattgc gtgagtcgcc aacaaacagc atctgttgtg gagcaatgcc
     9061 catccgttca gccaccagta acagcgggtc cggatgcggt tttttgtttt gcacatcatc
     9121 accaccaatc accacgctga agtatttggc gatatctaag gcttcgagca gcggcgcgac
     9181 gaacggcgtc ggtttgttgg tgaccaggcc tagcggcagg cctttagcct gcaacgcgcc
     9241 caacgtatcg gcaacatgcg ggaacaaaaa cgtcccctct tcggcaacct cgccatagta
     9301 gcgatcgaac agtttacgca gaatacgtac ctgttcttct gccggaatgt cgtcatcaac
     9361 gggcggttta cccattgttt tacgcagagt cgcacgttcc tgacgcgccc aggtcaatgc
     9421 gcgctccatc agaatatctg cgccgttacc aatccaggta ataacgcgtt cttcacctgc
     9481 gacaggcaac tccagcgcat acagcgccat atctaccgca gcagcaagac cgggtgcact
     9541 gtcaaccagc gtaccgtcga gatcaaaagc gacgccgcga atatcttcaa acttattcat
     9601 gacttacctt tgccagttca ctgcgcattt catcaatgac ttttttgtag tctggctggt
     9661 cgaagattgc cgaaccagca acaaacatat ccgcgcccgc cgcagcgatt tcgccaatgt
     9721 tgttcacctt cacgccaccg tccacttcca gtcgaatgtc aaagccagac tcgtcgatac
     9781 gacgacgtac ttcgcgcagt ttatccagtg tttgaggaat gaaagactga ccgccgaaac
     9841 cagggttaac agacatcagc aggatcacat ccagcttgtc cattacataa tccagatagc
     9901 tcaatggtgt cgccgggtta aataccagac ccgctttaca gccattttct ttaatcagtt
     9961 gcagcgtgcg gtcaacatgc tcggaggctt ctggatgaaa ggtaatgatg ctggcaccag
    10021 cggcagcgaa atcaggcaca atgcgatcga cgggtttcac catcagatgt acgtcgatag
    10081 gggcggtaat gccgtagtta cgcaaggatt tcagcaccat tggcccaatc gtcagattgg
    10141 gaacatagtg gttatccatg acgtcaaaat gcacgacatc tgcacccgct gccagggctt
    10201 ttgcggtatc ttcacccagg cgggcaaaat cagccgacag aattgagggg gcaatcaaat
    10261 actgtttcat ccgcttctcc ttgagaatta ttttttcgcg ggtgaaacga ctcctggttt
    10321 gtacaaagcc agcagttcgt ccaccttttt acgtgtgccg ccgttgctgc ttatactgcg
    10381 tcgaactttg acgacatgca attttgcgcg ctgataccac tcacgcgtta acatcgtatc
    10441 gtgattggag atcagcactg gaatatggcg atcaaccaga ccttcagcga tctccgccag
    10501 atgcgcttgt tgttcaagcg taaaactgtt tgtgtgatac gccgtaaagt tggcagtcgc
    10561 agacagcggc gcataaggcg gatcgcaata gacgacagat gcatcatctg cgcgcgccat
    10621 gctatcggcg taagactcac aatagaaaaa ggcattctgc gctttttcag cgaagtgata
    10681 caactctgct tccgggaaat agggtttttt gtagcggcca aacggcacgt taaactcacc
    10741 gcgcagattg taacgacaca ggccgttgta accgtagcgg ttcaaatata aaaacagtac
    10801 cgcccgacgg aacggatcct ggcttttgtt gaactcttcg cggaactgat agtaaacctc
    10861 ggcgcaattt gtttcgggaa caaatagctc gcgtgcggcc tgtacgtact catcagtacg
    10921 catcttcaca atgttataga gactgatcag gtcgctattg atatcggcaa ggatataacg
    10981 agaaaagtcg gtgttgagaa acaccgaccc ggcacctaca aaaggctcaa ccagacattc
    11041 gcccttgggc aaatgccgtt taatatcatc aagcagggga tacttgcccc ctgcccactt
    11101 caaaaaagcg cgatttttct tcatgctgac taactaatta caccttctcc ggctgtggag
    11161 aaagctccga cagcatccag cgcttcaggc aactcccgca atgataaacc attgcgggag
    11221 ataaccttga ttacttcaga tcggcctgta cctgacgcag cggtttcgcc cacgggtttt
    11281 tggcctggac atctgctggc aatgtagata ccgctttttt cgcctcttct ttcgaagcat
    11341 acacgccaga aaccaggaca taccacggct gaccattacg cgtcgtttca tagacaacgt
    11401 agtttttcag attctctttc ttcgcccaac cgttcaggtt gtcgtagtta gaggaactgc
    11461 tcagctgcag agtgtaatgg ctggacggtg ccgatttcaa cgaaccaaca ttacctgcgg
    11521 tcttccctcc agcggctggt gttgccgtgg tttgcgccgg ggatgccgtc tgtactggag
    11581 ccgtagtcgc cgtctctttt ggtgctggcg tagaagtcgc agccggtgcc tttgtgctcg
    11641 ccacaggggc agctggttca gttcgcttcg gcgtctgtgc gactggcttc ggttccgttt
    11701 tcacggttgc ttgcggtttt ttcggttcaa tcaccgcctg ctgacgagct gggcgcgtag
    11761 tggccggacg ttcagcggtt tgcgttttcg ccgtgtcacg cgatgcattg ccattgcgaa
    11821 caggcgcaac cgttgcgggt tcggtcggca atgtagaatt gaccgccaca ttgttcaact
    11881 gttgctgatt ttgtggctgg gtcagcgcat tgttcaggtc accctgcact tcaacacgtt
    11941 gttgaccatc cgttgccgcc ggggtttgcc cttgagtcgg cgtagaagag atcggcggca
    12001 gagaaacatc ctgctgagta ttttccgcag acgtggttcc cggcgctggc tgcacgccat
    12061 tggcctgatc ggtcgcattg cctgcaagat caatactctt ctcgccagac gcagtttgat
    12121 cgctggaaga ggtcgagggg gcttttagcg cagaaccgat accgatgatc aacagcagta
    12181 gaaccagaat gccgacaccc atcatcatat actgacgaga agcgggttta ctggctgctt
    12241 ttttacgctt acgcggacga cgctctacgc gctcttcatc cacggtttca tcttcggatt
    12301 cgtcaatttc ttcttcgatt tccggttcct cattacgcgc tttttgcgca cgagtcggac
    12361 ggcgatcgtc agtgtcatca agttcaatat catcaaaatt gatctgcggt tcgccacgtt
    12421 cagtacgctc agaacgttca gaagattgac gagaacgacc agtacgacga tcgctgggat
    12481 cgggtttcag ctcgtcttct ggtttgaatt catccattta acaccccact aaaaggttaa
    12541 tgcttaccac gttgcaatta acctgaagct aatagaccgc ttgataagcg gcctgacctt
    12601 tcttgttgtt acgctgattg acaatcggca atggcgttaa gaacaagctc gtgcgaaacg
    12661 ccgctgcgaa cttcactctt accaattgcc aacggaagaa ttaagcgcat ctctcccgca
    12721 aggactttct tgtcacgcag catatgcggt aaatacgcct gcgcggacat ttcgcgcggc
    12781 ccattgaccg gtaacccagc ccgcgtgagc agggttataa tacgctgcgt ttcggcagaa
    12841 ctaaaccgcc cgagacgttc cgacgtccgc gccgccatca ccatacccgc agcgaccgct
    12901 tcaccatgta accaattgcc ataccccatt tcagcttcaa tggcatgacc aaaggtgtgt
    12961 cccagattca gtaaagcacg taacccggtt tcgcgctcgt cggcggcgac aacttctgct
    13021 ttcagctcac aacaacggcg aatacagtac gccattgccg gaccgtccag acgcaacaac
    13081 gcatccagat tctcttccag ccagttaaaa aacgcaccgt caagaataat gccgtatttg
    13141 atgacttctg ccagccccga cgctaactca cgcgggggaa gcgttttcag acagtcgaga
    13201 tccaccacca ctgaagcggg ctggtagaac gcgccaatca tgtttttacc gaggggatgg
    13261 ttgaccgcag ttttgccgcc aacggaggaa tcgacctgcg acagtaacgt cgtcgggact
    13321 tgaatgaaac gtacaccgcg ctgataactc gccgccgcga agccggtcag atcacccact
    13381 acgccgccgc caagcgccac cagagtagta tcgcgaccat gcggcttttg taacaatgcc
    13441 gtaaagacgg tatcgagtac agccaggctt ttatactgct cgccgtcagg gaggataacg
    13501 ctatcgacgt taacacccgc ctgttcaagt acgccgcgga ccttatcgag atacagagga
    13561 gccagggttt cgttggtgac caacatgacc tgctcgcccg atttcagcgg taagaatgaa
    13621 gctggttcat taaacaaacc agatgcgatg gtaattgggt aactacgttc cccgagagta
    13681 acgacaatcc tctccataac gcgacatcca ccttaattac tgtacccgca gacgagtgta
    13741 tataaagcca gaattagttg ctttccagca tgtgaataat ctggtttgca accactttag
    13801 cgctttgatc atcagtacga atggtcacgt cggcaatctc ttcatacagc ggattgcgtt
    13861 cattggccaa cgcttccaga acttcacgcg gcggtgtttc aacgtgcagc aacgggcgtt
    13921 ttttatcacg ctgcgtgcgt gcaagttgct tttcgatggt cgtttcaaga taaacgacaa
    13981 cgccacgagc ggaaagacgg ttacgcgttt cacgggattt cacagagccg ccgccagtag
    14041 ccagcacaat accctgtttc tcggtcaact cattgatgac cttttcttcg cgatcgcgga
    14101 agccttcttc gccttctaaa tcgaaaaccc agcccacatc agctccggtt cgtttctcaa
    14161 tctcttgatc ggaatcgtaa aattccatat tgagttgttg agctaactgg cgcccaatag
    14221 tgctttttcc ggcacccata ggcccaacca gaaagatatt gcgtttctct gccatttttt
    14281 cggtactact aagactattc gttaatgata aacccgcttc gctcagagag cgccgcagga
    14341 catgaactga aacctcataa gatattgcga gagtcagact gaaaattatc tcaatactcc
    14401 agcgggtttg gcaactgaat aaatcaccca tccctctgca tatctggtcg ctgcaagcgc
    14461 gctgccttgc taccaccgct ctggcgataa atcaccgggt aagattagcg taaaaaagac
    14521 agcaaaatgc cgcctgaatg ataaatcatc atcatggaac gcaggcagcg aagagcacca
    14581 actctcaaat cggtactcct tgtatgctaa atacctgcgc gcgtcaaata gatgaaacac
    14641 gttcagcgta aaaacattac cgcttttacg gctgtttact cactggaaac cagtcgtggc
    14701 gtgataaaca ccactaactc gcgtcgttca tcttcttttc cgtcatgacg aaataattgc
    14761 ccgaaccagg gaatgtcgcc aagcaacggt acgctatcct gacccgattt atttttacgg
    14821 gtaaaaatgc cgcccagcgc caacgtttct ccgcttttga cctcgacctg cgtttcgatc
    14881 tcctgcttat caatcgccag cacttcgcca tctgcctgct gtagcacctg ccccggaacg
    14941 ttctggctga tgtgtaattt cagccggatg cgaccttttt gtaacaccgt gggcgtgact
    15001 tccatcccca ggacggcctc tttaaattcc accgacgtcg cgccactttc cccgctggaa
    15061 acctgatatg gaatttcgct cccctgttta atgctggcag gctgaagatg tgaggccagc
    15121 agacgcggac tggcgataat atccagctgc tgtttttgtt cgagcgcgga aagctcaaga
    15181 tccagtaaac gtccgttgat gcgtccaatg ttaaaaccga tatgcgttgt cgccgtcgct
    15241 acggagaggt cgctgccaag cgtggtgact tgcccaacgc caccagcttg ttgcgcatcg
    15301 gccagcgtcc atttcacacc taactcacgc aaactttttt cattaatggt gacaatatgc
    15361 gccgacagct caacctgccc gaccggcaga tccatttgcg atacccactg ttcaagcgtg
    15421 cttaacgccg ttttgttatc tcgcagcaaa aggcgattgg tgcgtttatc gacggtcata
    15481 ctccctttgg cactcagtag cttctccccc gctttcgcca gctctccggc gtcggcgtat
    15541 tgcagagtaa tattgcgatt ttccagcggc agatttgcct gcgcccgcgt ctgctccgcc
    15601 tcctggcggg cgatattgtc attctgccag gcaacggaat gcaccgagag aatgttgccc
    15661 tcctggcgcg ttatcaatcc ggcgcttttc actacagttt gtagtgcctg cttccaggga
    15721 acgtcagtta aatgtaacga caccgtaccg ctgacgtctg gcgaaaccac caggttcaac
    15781 ttctcctgtt cagccagcgc ctgcaacacc tgagctaccg gaacgtcatc caccatcagc
    15841 gtcacttttt gcggctttgc cgcctgtacg ccgggtatca gcatcaacag tagtgcggct
    15901 atccattgct tcatttgtat ctccttgccg ttgccacaac cagtgtggcg gttcgcagtt
    15961 tgtcccggtt cccagcgtta gtgcgtctgg cgtcagctgt aagatcgtcc agccgttttc
    16021 cagcacatcg ttttgctgca cccgtcgcca ttttttttgc ccatctttta ttacaccgat
    16081 gatgcgctcg cctcgcccta ccatcccctg atagcgccac tggctaagtt cgctaatccg
    16141 gcatagatct tccggcggtt taaaagggtc acgcataccg gttaaaaggc acaatgcaat
    16201 acctgccaac aaccagcgtt taaccctcat gcggcgtctc cagttgtagc gtgaacaaaa
    16261 ggtcatcacc ttccacgctt aacgaaaaac ggctcacgct gacgttgcgc tctgccagcc
    16321 gtgtaaatgc cgacggcact gcttcccaca gcgttttcaa cgccaactca ccaccctgcg
    16381 cggatggatg ccaggaaacc agttgcgcgc cggataactg aaaatccagt ggcgaaaagg
    16441 gcagcgtttt ttcttcgctg aagggagtgg cgtctaccag gcgatacagt gcagcccact
    16501 gctggtgatg actcgcccgt agtcgaatta atgcgtcgcg ctcttcctgg tgtgtcgatg
    16561 acagaaaaat gagcgtaact aacaccagca accagactgc ccagcaaagc tggcggaggc
    16621 ggagtgatgt ggcgaaccac cagtcaaaga acatgttcat cgctaacctt ccttgttaac
    16681 tgatactcaa attgccagcg tccctgcgca tcctgctgcg tggccccccg ctgattgaga
    16741 tgaaaagaag catcctggcg gagtgacgtt tccagcgcgt ttaacgcggt aatgcttgtt
    16801 gtaagcccct tgatctccag cgttccctgc tgccagctta tcgttgtcag ccaggcatgt
    16861 tcgggtaaaa gcgccgctag tgcttccagc gcagattgcc agtcgcgggt aaattgtcgc
    16921 tggcgctgac gttgcaaacg ctgttcgcgt aactgctggc gttccagcaa acgtggtttc
    16981 gttatctgta agctgcgggt gagttgttgt tccgcctgaa gcaaaacggc gtttacgcgt
    17041 gcttcggcat tgcttgtcag acgcagtatt agcgttatcc cgacggccag cagcaaaggc
    17101 gcaacgaaca tcagcaacca gaaacgaaga aaagcggtcc ggcgttgctg tcgccagggc
    17161 aaaaaattaa ttggcgggtt catcagtaca ccttcccaag cgccagcccc agcgcgatgg
    17221 caaagtctcc acctggtggc ggtagcggcg gctgacgaac agaaaccgcc tcccaggggt
    17281 caaatccgcc ttcgccacat agcgcgacgc tttcgggatc aacagataac gctgccgcca
    17341 gctccttcgc gctagtcatc cccaccgcca gtttgcgccc ccagctatag cgcgtcgccc
    17401 acagccactg ttcgttatca cgccaggcca gacattgctg atgagaaggt aaaaaaggca
    17461 ggaatcgctg taatgcactg gcatccggag tgatcgcgct aacatgaaca cgcaaccttt
    17521 ctgccagagt aagcagcgtt gccagctctt tgctttgcgc ggcagtcacg ttataggcgg
    17581 gactgagtga gtcttcgctg taatcgaagc gcagggagtc cggatccata tccagttcac
    17641 gggccatcgt cccggacagc caggccattt gctcccgctc accgagggac atcgacgggc
    17701 gtggaaatga ccgctgtaat gtgcgactgg cgggaaacgc caacataatg tgatgacgct
    17761 gcggcagttc gcgactccac ggtaacaacg tgttagccag ccgctgcaca tcaacaatcc
    17821 gcccatcttt gataatgtcg ttctccagtg gcaaccgcca ccagcggtgc aataagcatt
    17881 cttttgcgct ccgcacgata gcaaccgcta ccgcttcttg ctgttgtaaa tgcaaaccaa
    17941 tttgccagtt cttaaatgcc attgtgatga tctccttatc acccgtcact ctgacgggta
    18001 tatcaatgcg tctggcttgc ctttatacta ccgcgcgttt gtttataaac tgcccaaatg
    18061 aaactaaatg ggaaatttcc agtgaagttc gtaaagtatt ttttgatcct tgcagtctgt
    18121 tgcattctgc tgggagcagg ctcgatttat ggcctatacc gctacatcga gccacaactg
    18181 ccggatgtgg cgacattaaa agatgttcgc ctgcaaattc cgatgcagat ttacagcgcc
    18241 gatggcgagc tgattgctca atacggtgag aaacgtcgta ttccggttac gttggatcaa
    18301 atcccgccgg agatggtgaa agcctttatc gcgacggaag acagccgctt ctacgagcat
    18361 cacggcgttg acccggtggg gatcttccgt gcggcaagcg tggcgctgtt ctccggtcac
    18421 gcgtcacaag gggcgagtac cattacccag cagctggcga gaaacttctt cctcagtcca
    18481 gaacgcacgc tgatgcgcaa gattaaggaa gtcttcctcg cgattcgcat tgaacagttg
    18541 ctaacgaaag acgagatcct cgagctttat ctgaacaaga tttacctcgg ttaccgcgcc
    18601 tatggtgtcg gtgctgcggc acaagtctat ttcggtaaaa ctgtcgacca actgacgttg
    18661 aacgaaatgg cggtgatagc cgggctgccg aaagcgcctt ccaccttcaa cccgctctac
    18721 tcaatggatc gtgccgtcgc gcggcgtaac gtcgtgctgt cgcggatgct ggatgaaggg
    18781 tatatcaccc aacaacagtt cgatcagaca cgcactgagg cgattaacgc taactatcac
    18841 gcgccggaga ttgctttctc tgcgccatac ctgagcgaaa tggtgcgcca ggagatgtat
    18901 aaccgttatg gcgaaagtgc ctatgaagac ggttatcgca tttacaccac catcacccgc
    18961 aaagtgcagc aggccgcaca gcaggcggta cgtaataacg tgctggacta cgacatgcgc
    19021 cacggctatc gcggcccggc aaatgtgctg tggaaagtgg gcgaatcggc gtgggataac
    19081 aacaagatta ccgatacgct gaaggcgctg ccgacctatg gtccgctgct gcctgccgca
    19141 gtcaccagcg ccaatcctca ggaagcaacg gcgatgctgg cagacgggtc gaccgtcgta
    19201 ttgagtatgg aaggtgttcg ctgggcgcgt ccttaccgtt cggatactca gcagggaccg
    19261 acgccgcgta aagtgaccga tgttctgcaa acgggtcagc aaatctgggt tcgtcaagtt
    19321 gacgatgcat ggtggctggc gcaagtgccg gaagtgaact cggcgctggt gtcgatcaat
    19381 ccgcaaaacg gtgccgttat ggcgctggtc ggtggctttg atttcaatca gagcaagttt
    19441 aaccgagcca ctcaggcact gcgtcaggtg ggttccaaca tcaaaccgtt cctctacacc
    19501 gcggcgatgg ataaaggtct gacgctggca agtatgttga acgatgtgcc aatttcccgc
    19561 tgggatgcag gtgccggttc tgactggcag ccgaagaact caccaccgca atatgccggt
    19621 ccaattcgct tacgtcaggg gctgggtcag tcgaaaaacg tggtgatggt acgcgcaatg
    19681 cgggcgatgg gcgtcgacta cgctgcagaa tatctgcaac gcttcggctt cccggcacaa
    19741 aacattgtcc acaccgaatc gctggcgctg ggttcagctt ccttcacccc aatgcaggtg
    19801 gcgcgcggct acgcggtcat ggcgaacggc ggcttcctgg tggacccgtg gtttatcagc
    19861 aaaattgaaa acgatcaggg cggcgtaatt ttcgaagcga aaccgaaagt agcctgcccg
    19921 gaatgcgata ttccggtgat ttacggtgat acgcagaaat cgaacgtgct ggaaaataac
    19981 gatgttgaag atgtcgctat ctcccgcgag cagcagaatg tttctgtacc aatgccgcag
    20041 ctggagcagg caaatcaggc gttagtggcg aagaccggcg cgcaggagta cgcaccgcac
    20101 gtcatcaaca ctccgctggc attcctgatt aagagtgcct tgaacaccaa tatttttggt
    20161 gaaccaggct ggcagggtac tggctggcga gcaggtcgtg atttacaacg gcatgatata
    20221 ggcggaaaaa cgggtaccac taacagttcg aaagatgcgt ggttctcggg ttacggtccg
    20281 ggcgttgtga cctcggtctg gattggcttt gatgatcacc gtcgtaatct cggtcataca
    20341 acggcttccg gagcgattaa agatcagatc tcaggttacg aaggcggtgc caagagtgcc
    20401 cagcctgcat gggacgctta tatgaaagcc gttcttgaag gtgtaccgga acagccgctg
    20461 acgccgccac cgggtattgt gacggtgaat atcgatcgca gcaccgggca attagctaat
    20521 ggtggcaaca gccgcgaaga gtatttcatc gaaggtacgc agccgacaca acaggcggtg
    20581 cacgaggtgg gaacgaccat tatcgataat ggcgaggcac aggaattgtt ctgattaaaa
    20641 aggcgcttcg gcgccttttt cagtttgctg acaaagtgca cttgtttatg ccggatgcgg
    20701 gtgaacgcgt tatccggcca acaaaaaagt gaaaattcaa taaattgcag gaacttcgta
    20761 agcctgataa gcattatgca tcaggcaaac ttcacgcatt tacactcgcc cctgcccttt
    20821 caaccattcg cgcacgagga acagcgcgct gacattacgc gcttcgttga agtcaggggc
    20881 ttccagcaaa tccatcatat gcgccagcgg ccagcgcacc tgtggtaacg gctctggctc
    20941 atcgccttcc agtgattccg ggtagagatc ttgcgctacc acgatattca ttttgctgga
    21001 aaagtaagac ggtgccatgc tgagcttctt caaaaaagtc agatcgttcg caccaaatcc
    21061 aacctcttct tttagctcgc ggttagcggc ttcaaagacg ctttcacccg gatcaattaa
    21121 tcctttcgaa aaacctaatt cgtaggattc agttcccact gcgtattcgc ggatcaggat
    21181 caggtgatcg tccacaatcg gcacaatcat cactgcttcc cggttggttg gacgcattcg
    21241 ttcataaaca cgccgcacgc cattgctgaa ctccagatcc acgctttcga cggtaaacag
    21301 tcgggaacgg gctacagttt caacattcag aatggtgggt ttttgtaatg atttgctcat
    21361 cgtgggatct atgctgtgaa atcagcggtt attgtgcgat atcggacacg ctttcggcaa
    21421 tgtgaattgc atgttattta catttatgta acttaataaa taatcgtcct caaatcaaat
    21481 taaaagtcaa taggttgaaa taactccagg aatttgctga tattccgcct tcagagggtt
    21541 tgctatgatc agcggttact gtgatgtgct taatgatgct caagttaaac tccacgcttg
    21601 ccgatagcca accgcagaat catgtattgt gtccggtgcg actgaccacg cctgacagac
    21661 taagtaagat ggggaaagca tgagcaccat tgtgattttt ttagctgctt tgctggcctg
    21721 ctcactactt gcgggatggc tgataaaagt gcgatccaga cggcgtcagc tgccctggac
    21781 caacgccttc gcggatgcgc aaacgcgtaa actcacacct gaagaacgta gcgccgttga
    21841 aaattatctt gagagcctga cgcaggtatt acaggtgcct ggcccaacgg gagccagcgc
    21901 ggcaccgatc tctctggcgc tgaatgccga aagcaacaac gtcatgatgc tgacacacgc
    21961 tatcacgcgt tatggcatct ctaccgacga tccgaataaa tggcgttact acctcgattc
    22021 ggtagaagtc cacctgcccc ctttctggga acagtacatc aacgatgaga ataccgttga
    22081 actgattcat accgattcgc tgccgctggt tatttcactc aacggtcata cgctgcagga
    22141 gtacatgcag gaaacgcgcg gctatgcctt gcaacctgtt ccgtcaacgc aggcgtcgat
    22201 tcgcggggaa gagagtgagc aaatcgagct actcaatatt cgcaaagaaa cgcatgaaga
    22261 atatgcgctg agtcgtccgc gcgggctgcg tgaagcgttg ctgatcgtcg cctctttcct
    22321 gatgttcttt ttctgcctga ttaccccgga tgtctttgtt ccgtggctgg caggcggcgc
    22381 gttactgctg ctgggcgcag gtctgtgggg gctattcgcg cccccggcaa aatcctccct
    22441 gcgggaaatt cattgtctgc gcggtacacc ccgtcgttgg ggattgtttg gcgaaaacga
    22501 tcaggaacag atcaacaata tttcactcgg tattatcgac ctggtctatc ccgcacactg
    22561 gcagccctac attgctcagg atctcggcca acaaaccgat atcgatatct atctcgaccg
    22621 ccacgtagtg cgtcaggggc gatatctttc gctccatgat gaagtaaaaa actttccgtt
    22681 acagcactgg ctgcgcagta cgattatcgc tgcgggctcg ctactggtgc tgtttatgct
    22741 gttattctgg atcccgctgg atatgccgct gaaattcacc ctctcgtgga tgaaaggcgc
    22801 gcagaccatt gaagccacca gcgtgaaaca actggctgac gctggcgtgc gggttggcga
    22861 tacattgcgt attagcggta cgggaatgtg taatattcgc acttccggta cctggagcgc
    22921 gaaaaccaac tcaccttttt taccgtttga ctgctcgcag atcatctgga acgacgcccg
    22981 ctcattgcca ttaccagaat ctgaattggt caacaaagcg acggcattga ccgaagcagt
    23041 taatcgccag ctgcacccta aaccggaaga tgaatctcgc gtcagcgcct cattacgttc
    23101 agcaattcaa aaatccggca tggtattgct tgatgatttt ggcgacatag tactgaagac
    23161 agcggatctg tgttctgcca aagatgactg tgtgcgactg aaaaatgcgc tggtcaatct
    23221 cggcaacagt aaagactggg acgcgctggt aaaacgcgcc aacgccggga aactcgatgg
    23281 cgtgaatgtg ttattacgcc cggtgagtgc ggaatcgctg gataacctgg tggccacctc
    23341 taccacgccg tttatcacgc atgaaacggc gcgagcggca caatcactaa acagtccggc
    23401 ccccggcggg ttcctgattg tcagcgacga aggcagcgat tttgttgatc agccctggcc
    23461 ttcggcatca ctttacgact atccgccgca agaacagtgg aacgctttcc agaaactggc
    23521 acaaatgctg atgcatacgc cgtttaacgc cgaaggtatc gtcacaaaaa tcttcactga
    23581 cgccaatggt acgcagcata ttggccttca tccgatcccg gatcgttccg gtctgtggcg
    23641 ctatctcagc accacgctgc tgctactgac gatgctgggt agcgccattt ataatggcgt
    23701 acaggcctgg cgtcgttacc agcgtcatcg tactcgcatg atgaagattc aggcctatta
    23761 tgaaagctgc ctgaacccgc aactgatcac cccttccgaa agccttatcg aataacacgt
    23821 ttgcgcggca ggttatgcta ccctgtcgcg caaattgctt cactctggag atttccctca
    23881 tgcatatcaa cattgcctgg caggacgtag ataccgttct gctggatatg gacggcacgt
    23941 tgctcgacct cgccttcgat aactatttct ggcaaaagct ggtgcctgaa acatggggcg
    24001 cgaaaaacgg agttacgcca caggaagcga tggaatatat gcgccagcaa tatcacgacg
    24061 tacagcatac gctaaactgg tactgtcttg attactggag tgagcaactg ggtctggata
    24121 tctgtgcgat gaccaccgag atgggaccgc gtgccgtact gcgtgaagat accattccgt
    24181 ttcttgaggc actgaaagcc agcggtaaac agcgaatttt gctcaccaat gcgcatccgc
    24241 acaacctggc ggtaaaactt gagcataccg gtctggacgc acaccttgat ttattacttt
    24301 ccacccacac atttggttat ccgaaagagg atcagcggtt atggcatgcg gtggccgaag
    24361 ctacgggtct taaagctgaa agaacgctgt ttattgatga cagcgaagcg attctcgatg
    24421 ctgcagcgca atttggtatt cgttactgcc tcggcgtcac aaaccccgac tccgggattg
    24481 ccgagaaaca gtatcagcgc catccgtcac tgaatgacta ccgccgcctg atcccctcgc
    24541 taatgtgaag gagacgccat gaaagaaaaa cctgctgttg aggttcgact ggataaatgg
    24601 ctatgggccg cccgttttta taaaacccgc gcgctggccc gcgaaatgat cgaaggtggt
    24661 aaggtgcact acaacgggca gcgcagcaag ccgagcaaaa tcgtcgagct gaatgccacg
    24721 ctcactctgc gccagggaaa tgacgaacgc acggtgattg taaaggcgat tactgaacag
    24781 cgtcgccccg ccagcgaggc agtcttgctg tatgaagaga ctgcggaaag tgtagagaaa
    24841 cgcgaaaaaa tggcgctggc acgtaaactt aatgccttaa ccatgccgca tccggaccga
    24901 cgcccggaca aaaaagagcg ccgcgacctg ttacgattta aacacggcga cagtgaataa
    24961 ctgtcacctg caagagagat gattatgccg caacatgacc aattacatcg ctatctgttt
    25021 gaaaactttg ccgttcgcgg cgaactggta accgtttcgg aaaccctgca acagatcctt
    25081 gagaatcacg attatccgca gcccgttaaa aacgtgctgg cagaactgct ggttgcgacc
    25141 agcctgttaa ccgctacgtt gaagtttgat ggtgatatca ccgtacagct gcaaggcgac
    25201 ggtccgatga atctggcggt tattaacggt aacaacaacc agctgatgcg cggtgtggcg
    25261 cgcgtgcagg gcgaaattcc agaaaatgcc gacttgaaaa cgctggtcgg caatggttac
    25321 gtggtgatca ccatcacccc gagcgaaggc gaacgctatc agggcgtggt tggtctggaa
    25381 ggtgataccc tggcggcctg cctggaagat tactttatgc gttctgaaca gctgccgacg
    25441 cgcctgttta ttcgcaccgg cgacgtagac ggcaaaccgg ctgcaggcgg tatgttgttg
    25501 caggtaatgc ctgcgcaaaa tgcccagcag gacgactttg accacctggc gacgctaacc
    25561 gaaaccatca aaaccgaaga actgctgact ttaccggcaa acgaagtgtt gtggcgtttg
    25621 tatcacgaag aagaggtgac ggtttacgat ccgcaagatg tggagttcaa atgcacctgc
    25681 tcgcgtgaac gttgcgccga tgcgctgaaa acgctgcctg atgaagaagt cgatagcatc
    25741 ctggcggaag atggcgaaat tgacatgcat tgtgattact gcggtaacca ctatctgttc
    25801 aatgcgatgg atattgccga gatccgcaac aacgcgtctc cggcagatcc gcaagttcat
    25861 taatgagtat gtccggcaga gaggactctg ccggatttta cgacgtaatg gcctgatgcg
    25921 ctgcgcttac caggcctatg aattgacatc ctccacgccc tgaaggacgt ggatccctgc
    25981 tacgttcagg ctgtcgcctg aatcatttcg gtgggttcct gcttcaacgg gcggcctgac
    26041 tgcaccatct ctccacaggc aagaacggcg tgccccgccg ctaaaatgtt acgagcgcca
    26101 tttacatcgg cgttcgctgt atatccacat acctggcatc tgaattgact ttgtgacagg
    26161 cggttttctt tcgctgtatg accacagcac gcgcaacgct ggcttgtgta cgctggcgga
    26221 acagcaagca cctgaccgcc acgccagagc tgcttgtact caagctggcg gcgcatttca
    26281 taccagccct gatccagtat cgtacggttt aaacctgatt ttgcccggac attgcgcccc
    26341 ggctggctga ccgtacccgc tgctgacttt gacatgttgc tgaccttcaa atcctcaatg
    26401 actatcattg cgtggttttt gctgacggtc gttgtgactt tgtgaaggta gtccctgcgg
    26461 atatttgcga tacaggaatg tagtcgctgt attttgcgtt tctgcttctg ccagttgttg
    26521 ctgaatttga ccttgcggct taactgtcgc tgaagtctcg ccagcgtctt ctggtttttc
    26581 tgaaaactgt ttacaggctc aaagactgtg ccatctgaca gcgtggcgag tttagccacg
    26641 ccagcatcca gcccgaccat tgatgctgaa gggtgaaccg gagttgatac ttcactttct
    26701 gtctgaatac tgatgtacca cttaccgcag gactggctga cagtgacatt tttcacaact
    26761 cccgtgacct gacggctatt ccggtagcgc atccaaccaa gtttcggcag aaaaatacgg
    26821 ctgttttcct gatcgagctt aacgccctgc gggtagcgga atgcatcatt ctgtccccgc
    26881 tttttgaatc ggggaaaagc agcccgcttc tggaagaagt ttttgtaagc ccgctcaagg
    26941 tctttcagtg actgttgcaa tggctgtgag ggagaatctt taagccattg cgtttcagtg
    27001 gcatttttcc actcaaccag ccaggaagcc attttcccgt aagggatgta tttattaccg
    27061 gcctcatgat tctcattctg aagcgccaga gcacgattga aaacgaaacg acatgcgcct
    27121 gcgaagcgcc tcatttcacg ctcctgttga ccaccgggtc ttaaccggaa tgtgaatgct
    27181 gtagtcgttt cacagggcaa attatacttt ggtctgtgaa aaaaactgat atccgacgcg
    27241 gaaggcactg tgttttcctg atgcatgttc acctggtctt tgtcaccaaa taccaacgcc
    27301 agatttttga ccatgacgcg acagaaaaac tacgcactta cttttcaaat gtatgtgctg
    27361 attttgaagc tgaacgggtt gaaatggatg gcgaacttat atccccgccc tgaaggacgg
    27421 ggttttaccg cgcaccggat aaatcgcaag ttattttggt cagatacagc gtcgtatccg
    27481 ttaaaagcgt tgtcacttac ttaagtttat ttccagcgtt tcaccatcta ccgtcagatc
    27541 gcgccacgct ttcggcctcg tccccagttt tccatccttc gcagctgtga gtaatttttc
    27601 tttcgttatt ttgcgcggaa gtaaactcat atcaagcgcg tcccattctt ttctggctga
    27661 gtcaaagcca tagacaatga ctctgtcatc attaaaagca aacaggatcc gctccgcctg
    27721 accatcacta ttcagatctt gctcgaccag tacgcaggcg tctttttcaa tacaggtcat
    27781 cacgttatag cgatcctgga ttaaggccga ccagaatgtc gcatccggtt taccagaacc
    27841 tggggcaatt aacacattat cggctaatac tttttccgat acctgttcct gaagatgttg
    27901 ctctccatct aacgccatca gcagatcccg cgcgcgtttc gggtctttca tatactcggc
    27961 atcgcttttc agcgactcaa gcgccgcacg tccatagcga ccgctctgtt cgagcatgta
    28021 gatagttacc tggtctggcg tgtttttacc gctctgataa cgcgccatat gactgttcac
    28081 gctaatgcgc atactgtcca gcaccggcga attaagaagc accagtatta ccaacaccaa
    28141 taacgaaacc gcaaggttca ctttgccctg aagatcaagg ggattttgcc ctttacgcca
    28201 gacgatactg acaaaatacc ccagcgacca taccagtaac accagcaccg ccagcgcgcc
    28261 ctgcaagcgg tcgacagtcc agccgtactg agcgacccgc agccataacg cccaggcggc
    28321 gacgaacaca tacagcggag caaccagcaa agcggtttta atcaggcaac gcaatggccc
    28381 cgtccaggga agtgacgctt tttgcggatc gcggacaata gccattaaga tcaattgcaa
    28441 aaaggccagc gtcaacagca acccggcggc ggagatgtgg cgagaaatcg cgctcaggcc
    28501 cgtaaacggc agggtgataa taaacatcag ggttagcaat gatactaacg gcagcaaccc
    28561 cgtggcgatt aacgtgaaca acttttgaat agagtcgatt aaacgtgact gtgttcgcgc
    28621 gaggatcacc gccagcgcgg taaccagacc taacgtgaga taaataaacc agtcggttgc
    28681 aaaaaagagc gttttgaaaa acgtgatgcc aacaagttta aacaactcac tccacagtaa
    28741 aagtaccagc cacgttaggc cattggcgag aaaaatcacc agtaatataa gtacgttatg
    28801 ccatactgac tggtaaaagt agcgatagcg gctactgtca ttgcggatgc gaaggctttg
    28861 ttgtatccac ggtagcaaca acattgccat cagcagcaga tagcagccaa aatcccaaag
    28921 cgccttttca gctctccagg gggtcatgcc atcagtttgc cacttcagcc agccgctcat
    28981 tcccagcgtg gcaataaaca ccagcgccag ccatccccaa aggcgattct gtttaaagga
    29041 gatcacggag aagagtaaaa cggatgaaaa cgcgacggtc gcaggcacgc cataaacaat
    29101 ccaactgaga ttttttccgg caagccaggc gatcagcagg tagcaaacca acccctgaag
    29161 taatccggta gcaatcatcc cccagcgtgt tgccggtgaa agttcaacgt tatccatgtt
    29221 gtttatccag aatcaaaagg tgggttaatt atcgcatccg ggcagtagta ttttgctttt
    29281 ttcagaaaat aatcaaaaaa agttagcgtg gtgaatcgat actttaccga ttgaatttgc
    29341 atcaatttca ttcatgaatg cgattccact cacaatattc ccgccatata aaccaagatt
    29401 taaccttttg agaacatttt ccacacctaa aatgctattt ctgcgataat agcaaccgtt
    29461 tcgtgacagg aatcacggag ttttttgtca aatatgaatt tctccagata cgtaaatcta
    29521 tgagccttgt cgcggttaac acccccaaaa agactttact attcaggcaa tacatattgg
    29581 ctaaggagca gtgaaatgcg cgttaacaat ggtttgaccc cgcaagaact cgaggcttat
    29641 ggtatcagtg acgtacatga tatcgtttac aacccaagct acgatctgct gtatcaggaa
    29701 gagctggacc cgagcctgac cggttatgag cgcggggtgt taactaatct gggtgccgtt
    29761 gccgtcgata ccgggatctt caccggtcgt tcaccgaaag ataagtatat cgttcgtgac
    29821 gataccactc gcgatacttt ctggtgggca gacaaaggca aaggtaagaa cgacaacaaa
    29881 cctctctctc cggaaacctg gcagcatctg aaaggcctgg tgaccaggca gctttccggc
    29941 aaacgtctgt tcgttgtcga cgccttctgt ggtgcgaacc cggatactcg tctttccgtc
    30001 cgtttcatca ccgaagtggc ctggcaggcg cattttgtca aaaacatgtt tattcgcccg
    30061 agcgatgaag agctggcagg tttcaaacca gactttatcg ttatgaacgg cgcgaagtgc
    30121 actaacccgc agtggaaaga acagggtctg aactccgaaa acttcgtggc gtttaacctg
    30181 accgagcgca tgcagctgat tggcggcacc tggtacggcg gcgaaatgaa gaaagggatg
    30241 ttctcgatga tgaactacct gctgccgctg aaaggtatcg cttctatgca ctgctccgcc
    30301 aacgttggcg agaaaggcga tgttgcggtg ttcttcggcc tttccggcac cggtaaaacc
    30361 accctttcca ccgacccgaa acgccgcctg attggcgatg acgagcacgg ctgggatgat
    30421 gacggtgtgt ttaacttcga aggcggctgc tacgcgaaaa ctatcaagct gtcgaaagaa
    30481 gcggaacctg aaatctacaa cgctatccgt cgcgatgcgc tgctggaaaa cgtcaccgtg
    30541 cgtgaagatg gcacaatcga ctttgatgat ggttcaaaaa ccgagaacac ccgcgtttct
    30601 tatccgatct atcacatcga taacatcgtt aagccggttt ccaaagcggg ccacgcaact
    30661 aaagttattt tcctgacagc agacgccttt ggtgtgctgc cgccggtttc tcgtctgact
    30721 gccgatcaaa cccagtacca cttcctctct ggcttcaccg ccaaactggc tggtacagag
    30781 cgcggcatca ccgaaccgac gccaaccttc tccgcttgct tcggcgcggc gttcctgtcg
    30841 ctgcacccga cccaatacgc agaagtgctg gtgaaacgta tgcaggcggc gggtgcgcag
    30901 gcgtatctgg ttaacaccgg ctggaacggc actggcaaac gtatctccat taaagatacc
    30961 cgcgctatta tcgacgccat cctcaacggt tcgctggata acgcagaaac cttcactctg
    31021 ccgatgttta acctggcgat cccaaccgaa ctgccgggcg tagacacgaa gatcctcgat
    31081 ccgcgtaaca cctacgcttc tccggaacag tggcaggaaa aagccgaaac gctggcgaaa
    31141 ctgtttatcg acaacttcga taaatacacc gacacccctg cgggtgccgc gctggtagcg
    31201 gctggtccga agctgtaatg atttgaagct ggagaatatc tatccagtat cttatagaaa
    31261 gcaaaacggg aggcaccttc gcctcccgtt ttatttaccc ttcttttgtc gtgccctgcg
    31321 cccgcgttac cggcactggc agccaggcgc gaatggaaag cccgccccgt tcgctggtgc
    31381 caagctccag catcccgtta tggttatcca cgatacgctg cacaatcgcc agccctaatc
    31441 ccgtgccgct aatagtgcgc gcactgtcgc cacgaacaaa cggctggaac aggtgcttac
    31501 gttgttccgg cgcaataccc ggaccgtcat cttctacctg gaaccaggcg cgattcggct
    31561 ccgtgccgct gctgactttg atccagccat tgccgtaacg ggcggcgttg accaccatat
    31621 tcgctaccgc gcgtttgatc gacagcgggt gcattttcac ctcaatgcta ccggggtaaa
    31681 gcgcagtttc aatttcccgc tcatagccac tttcggcagc aataacctca ccgagtactg
    31741 cgttgagatc cgccatttcc atcggcatct cctgcccggt gcgcaggtag tcgataaact
    31801 gctcaatgat ggcgttgcac tcttcgatat ctttattgat cgattctgcc aggtagccat
    31861 cctgctcgct catcatctcg gttgccaggc gaatacgcgt cagcggcgtg cgcagatcat
    31921 ggcttacccc cgccatcagc agcgtgcggt catccgccag ttgcttaaca ccagccgcca
    31981 tatgattaaa ggcgcgggta acggaacgca cctccgaagc gccatattca cgcagcggcg
    32041 gcggaataat ccctttaccc acctgcaagg ctgcgtgttc gagatcgacc aacggtcggt
    32101 tctggatacg aataaacagc cacgccccgc ctatcgccaa tagcataatc gccaacgtat
    32161 aacggaacag cggggagaaa tcgccctgat gaatttcggt tagcggcacg cgtacccaga
    32221 tattgggaga caaccaggtt ttcagccaga cgacaggcga acttttgttg acctcgacgc
    32281 gaacttccgt cggaccgccc agttgctgcg ccatctgatg gcttaagaat tcatagtgtt
    32341 gcgcccaacg cagacctgcc tcttcggcag cttcgttgga atagagagag atccccagct
    32401 cacggtagat ctcccgacgg aaagcgggag gcacaaccaa ctgcgtgccg tcctccagtt
    32461 gcagtttgtc ggtcatcaac atacgcactt cgtacgcgag gactttatta aactgctgga
    32521 ggctcggcaa aatcgcgaag ttcagcacca ccagataagt cgtcaccagg ctgacgaaca
    32581 gcaaggtgac gatgagcaat aatgtacggg caaatgaact tcgtggcgag aagcgcaatc
    32641 gcctcatgct ttagaaccgt ccggtacaaa gacgtagccc agaccccaga cggtctgaat
    32701 gtaacgcgga tgcgccggat cttcttccac catgcggcgc agacgcgaaa tctgcacgtc
    32761 gatggagcgt tccattgcgg aatattcacg accacgggca aggttcatca gcttatcgcg
    32821 ggagagcggc tcacgcggat ggctgaccag tgccttcagt accgcaaact caccactggt
    32881 gagcggcatc ggctcgtctt cgcggaacat ttcgcgcgta ccgaggttaa gtttgaactt
    32941 accgaaagca attaccgcct cttcctgtga cggtgcgcct ggcagttcgt tcgcctgacg
    33001 acgcagcacc gcacgaatgc gggccagcag ttcacgcggg ttaaacggtt ttggaatgta
    33061 atcgtcagcg ccaatctcca ggccaacgat acggtccact tcttcccctt tcgccgtcac
    33121 cataatgatc ggcatcgggt tactctggct acgaagacgt cggcaaatcg acaagccatc
    33181 ttcaccaggt aacatcaaat ccagtaccat aagatggaaa gattcacgag tcagcaggcg
    33241 atccatctgt tctgcattag cgacgcttcg aacctggaag ccttgttcgg tgagataacg
    33301 ttccagcagc gcgcgcaggc gcatgtcgtc atcaaccacc agaatcttgt agttctcttg
    33361 cattgtttgt actcccaaag gttcgcaaca atttgtaagc gtgtattctt aaaaaagctc
    33421 acgttcgtca ccagctaaat ctggtatgaa tttcagccta aattgttaca aagcatatta
    33481 aacagcagct taagtataca atttattcgg cgaaacatta ttgattctgt tgatatgatc
    33541 acgttatacc caatgtgcgc attatcaaac agacaaaggg aatcaacgag atgaaaacgc
    33601 ccctggtaac ccgggaaggg tatgaaaaac tcaaacaaga gcttaattat ctctggcgtg
    33661 aagaacgccc ggaggtcaca aagaaggtga cctgggccgc aagtctgggc gaccgaagcg
    33721 aaaatgctga ctatcagtat aataaaaagc gcctgcgtga aatcgaccgt cgcgtgcgct
    33781 atctcactaa atgcatggaa aatctcaaaa tcgtcgatta ctccccgcag caggaaggca
    33841 aagtcttttt tggtgcgtgg gtggagattg aaaacgacga tggtgtgact caccgtttcc
    33901 gtattgtcgg ctacgatgaa atttttggcc gtaaagatta catctctatc gattccccga
    33961 tggcccgcgc attgctgaaa aaagaagtcg gcgatctggc ggtggtgaat acccctgccg
    34021 gggaagcgag ctggtatgtt aatgctatcg agtacgtgaa accgtaagga agagtcttaa
    34081 cctcctgccg atggctggca tttttgccag ccagtccgta taactatccc ctgatttttg
    34141 atccgaaaag atgaactcaa accatgatga atgattcgtt ctgccgcatt attgcgggtg
    34201 aaattcaggc gcgcccggaa caggttgacg ctgccgttcg cctgcttgac gaagggaata
    34261 ccgtgccgtt tatcgcacgt tatcgtaagg aaatcaccgg cggtctggat gacacgcagc
    34321 tgcgtaatct ggaaacgcgt ctgagctatc tgcgcgagct ggaagagaga cgtcaggcga
    34381 tcctcaagtc catttccgag caaggcaaac tcaccgatga tctggcgaat gccatcaacg
    34441 ccactctaag caaaaccgaa ctcgaagacc tctacctgcc ctacaaacct aaacgccgca
    34501 cccgtgggca aatcgccatt gaagcagggc ttgagccgtt ggccgatctg ctgtggagcg
    34561 atccgtcaca cacgccagaa gtcgccgctg cacaatatat tgatgccgat aaaggcgtgg
    34621 cagataccaa agccgcgctg gacggcgcgc gctatatcct gatggaacgg tttgccgaag
    34681 atgccgcgct gctggcgaaa gtgcgtgatt atctgtggaa gaacgcgcat ttggtctcaa
    34741 ccgtggtgaa tggtaaagaa gaggaagggg cgaaattccg cgactatttc gatcatcacg
    34801 aaccactgtc aacggtgcct tctcaccgcg cgctggcgat gttccgtggt cgtaacgaag
    34861 gcatacttca gctttcgctg aatgccgatc cgcagttcga agagccgccg aaagagagct
    34921 attgcgagca aatcatcatg gatcaccttg gcctgcgcct gaacaatgcc ccggcggata
    34981 gctggcgcaa aggtgtggtg agctggacgt ggcgcatcaa ggtgttgatg catctggaaa
    35041 ctgaactgat gggcaccgtg cgcgaacgcg cggaagatga agcaatcaac gtctttgccc
    35101 gtaacctgca cgatctgctg atggcggccc ctgccggact gcgtgcaacg atgggcctcg
    35161 atccgggtct gcgtaccggg gtaaaagtgg cggtagtcga tgcaactggc aaactggtgg
    35221 cgaccgatac catttatccg cataccgggc aggccgcaaa agcagcgatg accgttgctg
    35281 cgctgtgtga aaagcataac gttgaactgg tggcgatcgg caacggtaca gcttctcgcg
    35341 aaaccgaacg tttctatctc gacgtgcaga agcagttccc gaaagtgacc gcgcagaaag
    35401 tgatcgtcag cgaagctggc gcgtcggttt actcggcttc cgagctggca gcacaggaat
    35461 tcccggatct cgacgtttcg ctgcgtggcg cggtgtctat cgcccgtcgt ttgcaggatc
    35521 cgctggcgga gctggtgaaa atcgatccga aatctatcgg cgtaggtcag tatcagcatg
    35581 acgtcagcca gacgcagctg gcacgcaaac tggatgcagt agtagaagac tgcgtaaacg
    35641 ccgttggcgt cgatctcaac accgcttctg ttcctctgtt aacccgcgtg gcgggcctga
    35701 cgcgcatgat ggcgcaaaac atcgtggcct ggcgcgatga gaacggccag ttccagaacc
    35761 gtcagcaact gttaaaagtg agccgtctgg ggccgaaagc cttcgagcag tgcgcgggct
    35821 tcttgcgcat taaccacggt gataacccgc tggacgcttc taccgttcac ccggaagcct
    35881 atccggtggt ggaacgcatt ctggcagcaa cacagcaggc actgaaagat ctgatgggta
    35941 acagcagcga actgcgtaac ctgaaagcgt ctgactttac tgatgagaaa ttcggtgtgc
    36001 cgacggtaac cgacatcatc aaagagctgg aaaaaccggg tcgcgatccg cgtccggaat
    36061 ttaaaaccgc tcagtttgcc gatggtgtcg agacaatgaa cgacctgcaa ccgggtatga
    36121 tcctcgaagg tgcggtgacc aacgtcacca actttggcgc gtttgtcgat attggcgtgc
    36181 atcaggacgg cctggttcac atctcttcat tgtcgaacaa gtttgtggaa gatccgcata
    36241 ccgtggtgaa agcgggcgac attgtgaagg tgaaagtgct ggaagtggat cttcagcgta
    36301 aacgtatcgc cctgaccatg cgtctggacg agcagcctgg cgaaaccaac gcccgtcgcg
    36361 gcggcggtaa tgaacgcccg caaaacaacc gcccggcagc caaaccgcgt gggcgtgaag
    36421 tgcagcctgc cggtaacagc gcgatgatgg atgcgctggc ggcggcaatg ggcaaaaaac
    36481 gttaaacgcc cggactggcc cctaaacctg cgcgattagg cgttcacgcc acatctggca
    36541 acgaacgcct tgcctgacat aaaagtgccg gagaatatct ccggcctttt tattccacaa
    36601 gcaaacgcat aatatatccc gttaatattt atcacttcat taacaattga aaccttaatt
    36661 aaacattaac cagccctaat aattcgtcat gcgaattata ttttcgctgc gatataagct
    36721 tgagccatat caacattgag tcagattatt attcaaacca acattcacac acattttaag
    36781 tattgctgat agaaaccatt ctcattatca ttgtactgtt gattatttaa tctctccttc
    36841 gttggcaaat catctggtct catgtcgctg tcaaacgccc catgaggtag ttatccagtt
    36901 aatgagaaac aagtaggcac ctatgcaata cactccagat actgcgtgga aaattactgg
    36961 cttttcccgt gaaatcagcc cggcatatcg ccaaaaactg ctttctcttg gcatgttacc
    37021 tggctcctct tttaatgtgg tgcgcgtcgc tccactcggc gaccccattc atatcgaaac
    37081 ccgtcgtgtg agcctggtat tacgcaaaaa agatctggcc ttattagaag tggaagcggt
    37141 ttcctgttaa tacggtgata acaacaatga aaaaattaac cattggctta attggtaatc
    37201 caaattctgg caagacaacg ttatttaacc agcttactgg ctcacgtcag cgtgtaggta
    37261 actgggctgg cgttaccgtc gaacgtaaag aagggcaatt ctccaccacc gatcatcagg
    37321 tcacgctggt ggacctgccc ggcacctatt ctctgaccac catctcatcg cagacctcgc
    37381 tcgatgagca aatcgcctgt cactacattt tgagtggcga cgcagacctg ctgattaacg
    37441 tggtggatgc gtctaacctt gagcgtaacc tgtacctgac gctgcaactg ttggaactcg
    37501 gcattccctg catcgtggcg ctgaacatgc tcgacattgc cgagaagcaa aatattcgta
    37561 ttgaaattga tgctctgtcg gcgcgtcttg gctgcccggt gatcccgctg gtttcaaccc
    37621 gtggtcgcgg tattgaagcg ctcaagctgg cgattgatcg ctataaagct aacgagaatg
    37681 tggaactggt gcattacgct cagctgctgc tcaacgaagc agattcactg gcaaaagtga
    37741 tgccttccga catcccgctg aaacaacgtc gctggctggg cctgcaaatg ctggaaggcg
    37801 atatctacag ccgcgcctac gccggtgaag cgtcgcagca tctggatgcc gccctcgccc
    37861 gtctgcgtaa tgagatggac gatccggcgc tgcacattgc cgatgcgcgt taccagtgca
    37921 ttgctgccat ctgtgatgtg gtaagcaaca ccctgacggc agaacccagc cgtttcacca
    37981 ctgcggtaga taaaatcgtg ctcaaccgtt tcctcggtct gccgattttc ctctttgtga
    38041 tgtacctgat gttcttgctg gctatcaaca tcggcggggc gttacagccg ctgtttgacg
    38101 tcggctccgt agcactcttt gtgcatggta ttcagtggat tggctacacg ctccacttcc
    38161 cggactggct gactatcttc ctcgcccagg gcctgggcgg cggtattaac accgtgctgc
    38221 cactggtgcc gcagatcggc atgatgtacc tgttcctctc cttccttgag gactccgggt
    38281 atatggcgcg tgcggcgttt gtgatggacc gtctgatgca ggcgctgggc ttgccgggga
    38341 aatcctttgt accgctgatc gttggtttcg gttgtaacgt accgtcggta atgggcgcac
    38401 gtacgcttga tgcaccgcgt gaacgtctga tgaccatcat gatggcaccg tttatgtcct
    38461 gcggcgcgcg tctggctatc ttcgcagtat tcgcggctgc cttcttcggg cagaacggtg
    38521 cgctggcggt cttctcgctg tatatgctgg gtatcgtaat ggcggtgctg actggcctga
    38581 tgctcaagta caccatcatg cgcggtgaag cgacgccgtt tgtcatggag ctgccggtct
    38641 accatgtacc acacgttaaa agcctgatta tccagacctg gcagcgtctg aaaggcttcg
    38701 ttctgcgtgc tggtaaagtg attatcatcg tcagcatttt cctgagcgcc ttcaacagct
    38761 tctcactgag cgggaaaatc gtcgataaca tcaacgactc ggcgctggct tccgtcagcc
    38821 gggtgatcac cccggtcttc aagccgattg gcgtacatga agataactgg caggcaacgg
    38881 ttggcctgtt tacaggtgcc atggcgaaag aagtggtggt gggtacgctc aacaccctct
    38941 acaccgcaga aaatattcag gacgaagagt tcaatccggc ggaatttaac ctcggtgaag
    39001 agctgttcag tgcggtagat gaaacctggc agagcctgaa agacaccttc agccttagcg
    39061 tactgatgaa ccccattgaa gccagcaaag gcgacggcga aatgggtacc ggggcgatgg
    39121 gcgtgatgga tcagaaattc ggtagcgcag ctgccgcata cagctacctg attttcgtcc
    39181 tgctgtacgt accgtgcatc tcggtgatgg gggctatcgc gcgtgaatca agccgtggct
    39241 ggatgggctt ctccatcctg tgggggctga atattgctta ctcactggca acattgttct
    39301 atcaggtcgc cagctacagc cagcatccaa cttacagcct ggtgtgcatt ctggcagtta
    39361 tcctgtttaa catcgtggtt atcggtctgc tgcgccgcgc gcgtagccgg gtagatgtcg
    39421 aactgctggc aacgcgcaaa tcggtaagca gttgctgtgc ggccagtacc actggcgatt
    39481 gccactaagg acacgacaat ggcttcgctc attcaagtgc gtgatttact ggcattacgg
    39541 ggccgtatgg aagcggccca gattagccag acactgaaca ctccgcagcc aatgattaac
    39601 gccatgctga aacagctaga aagtatggga aaagcagtgc ggattcagga agaacctgac
    39661 ggctgtcttt ccggcagttg taaaagctgc ccggaaggaa aagcctgtct gcacgaatgg
    39721 tgggcgttgc gttaaccttg tttcactgcc tgttttgaaa gcccggtatg cgtttgcatc
    39781 cgggcttttt tgcgtgcggc tttccataaa aatgcaaccc ttgcagcgcg acgtaagttc
    39841 ctctgaaagc gtctcgcagg gatgaaaact cgctaatgca caggtgtgga gtggcgcgta
    39901 gagtcgcggc attcaaataa caggtgaagg aacgccatga gcaaaaagca gagttccacc
    39961 ccacacgatg cgctgttcaa actcttttta cgccaaccgg agacggcacg cgagcatggc
    40021 gctgctggag ttaattcaaa aacatattcg tcaacgcgat ctgatgggac tggtagagca
    40081 aatggcctgc ttattaagta gtggatacgc aaatgacaga caaatcaaag ggctgtttaa
    40141 ttacatactg caaaccggcg acgcggtacg ttttaacgat tttatcgatg gtgtggccga
    40201 acgttcacca aaacacaagg agagtttaat gactattgcg gaaagattgc ggcaggaagg
    40261 ggaacaatcc aaagccttgc atatagcaaa aataatgctt gaatccggag tccctcttgc
    40321 agacatcatg cgctttaccg gggtgtcaga agaagagttg gctgcggcga gccagtaaag
    40381 ttctgtcccg ccatttcgaa agccacctac accctctgct tcaacgccac cagcaggtga
    40441 caaaactcgt ccggatgtga aataaacggc gcatgggccg ctttggcgaa gatatatgat
    40501 tcgctgtgag gccaaagttt atccagcatc ggcaccactt tgcgcggcac cagaccgtcg
    40561 agatagccat acaatcgcaa aaacggcatg ggcacgtttt gcagcggctg acggagatcg
    40621 accgttttca ggatttccag cccgccattg agtacgtcaa cctccggcat cggtaacgcc
    40681 agaacggttt tcttcaacgc ccgcgcatcc tggcgcgccg tttcagtacc catggtttgt
    40741 aacgccagga accgctccac tgtacgctga aaatcatcac tgagttgctg ctgaaatccc
    40801 gccagcacgt ccggttttat ccccggccac tcgtcgcgag cactaaaaca aggtgacgac
    40861 gccacggtga ccagcgccag aacccgctcg ggatgggtta acgcaatctg gcttgccacc
    40921 agcccgccca gactccagcc taaccaaatg gctttatctg gtgcctgtcg cagcacgact
    40981 tcggccatat cagcaagtga tagcgcacca aatccccggc tacgcccgaa gccgggcagg
    41041 tcaacaaggt gcagcgtaaa atgcgagcta agttcctcgt caatgcaacg ccacacttcg
    41101 gcattcagtc cccatccgtg cagcagcaca agatgaacat tcccctgacc tttggtctgc
    41161 caccagatgt tattcatccg ctattgttct ctttttactt acaaggatga acatatgcta
    41221 acagtaccgg gattatgctg gctatgccga atgccgctgg cgttaggtca ttgggggatt
    41281 tgttctgtct gctctcgcgc cgcccgtaca gataaaacgt tatgcccaca atgtggatta
    41341 cccgccacac actctcatct tccctgcggt cgctgcctgc aaaaaccgct gccctggcaa
    41401 agactgctca cggtcgccga ctatgcgccg ccattaagtc cgctcattca ccagcttaag
    41461 ttttcccggc gcagtgaaat cgccagcgcc ctgtcacgtc tgttactatt ggaagtctta
    41521 cacgctcgtc gcaccaccgg cttgcaactg cctgatcgca tcatcagcgt tccgttatgg
    41581 cagcgccgtc actggcgtcg gggatttaat cagagcgatt tgctgtgtca gccgttatca
    41641 cgctggctac actgccaatg ggatagcgaa gccgtcacac gaacacgggc caccgcgacc
    41701 cagcattttc ttaacgcccg gctacgcaaa cacaacctga aaaatgcctt tcgccttgaa
    41761 ttgcctgtgc aaggtcgcca tatggtgatt gtggatgatg tcgttaccac cggaagtacc
    41821 gtcgcagaga ttgcgcagtt gcttttacgc aatggtgcgg cgactgtcca ggtctggtgc
    41881 ctttgtcgaa ccttgtagag cctcgatgat gggcgtatta taaccaacta aaatagtcaa
    41941 ctattaggcc attactatga tccgtatttc cgatgctgca caagcgcact ttgccaaact
    42001 gctggcaaac caggaagaag ggacacaaat ccgcgtattt gtgattaacc ctggcacgcc
    42061 taacgctgaa tgtggcgttt cttattgtcc gccggacgct gtggaagcca ccgacactgc
    42121 cctgaaattt gacctgctga ccgcgtatgt tgatgagtta agcgctcctt acctggaaga
    42181 tgcagagatc gattttgtta ccgaccagtt gggttcgcag ctgacgctga aagcgcctaa
    42241 cgccaaaatg cgtaaagtgg ctgacgatgc gccgctgatg gagcgcgtgg agtatatgct
    42301 gcaatcacag atcaacccac agcttgctgg tcacggtggt cgcgtttcgc tgatggaaat
    42361 caccgaagac ggttacgcca ttttgcaatt tggcggtggc tgtaacggtt gttccatggt
    42421 cgacgtcacc ctgaaagagg ggatcgagaa gcagttgctg aacgaattcc cggagctgaa
    42481 aggtgtacgc gatctcaccg aacaccagcg cggcgagcac tcttactact aagttctctc
    42541 cccttttgtc gattgcccga tgtgacgcta aagcgtctta tcgggcctgt acgttgcacg
    42601 agagtctgac gttagcgcag aattatctca gcaaatccaa tctcttcgtt tccccacgat
    42661 aatatgacca acctctcata atttaaattt accccgctct ggtgattctc aagcacgtaa
    42721 tgttacccgt atcattctca tgggcaccaa acacactcat aacatctgac tacactaact
    42781 cgttttaggg gtcatcagcc gtggtgccgt aacatctctg ttcccagttg ggacaaccag
    42841 gattttccgt cagaaacctg acgttaccca taacaaatta aaggccaggt aaatcatgcc
    42901 attagtcatt gttgctatcg gtgtaatctt gttattactc ctgatgatcc gcttcaaaat
    42961 gaacggcttc atcgctctcg tcctcgtggc gcttgctgtt ggattaatgc aagggatgcc
    43021 gctggataaa gttattggct ccatcaaagc tggtgtcggc ggaacgctcg gtagccttgc
    43081 cctgatcatg ggttttggtg cgatgctggg caaaatgctg gcagactgcg gtggtgcaca
    43141 acgtatcgct accacgctga ttgccaaatt tggtaaaaaa cacatccagt gggcggtggt
    43201 actgaccggt tttaccgttg gttttgccct gttctatgaa gtgggtttcg tgctgatgct
    43261 gccgctggtg tttaccatcg cggcttccgc gaatattcca ctgctgtatg ttggtgtacc
    43321 aatggcggct gcactgtctg tgacccacgg cttcctgcca ccgcatccgg gtccgactgc
    43381 gattgccacc attttcaatg ccgatatggg taaaactctg ctgtacggta ctattctggc
    43441 aatcccgacc gtgattctcg ccggtccggt ttacgctcgc gtgttgaaag gtatcgataa
    43501 gccgatcccg gaaggtctgt acagcgcgaa aaccttcagc gaagaagaga tgccgagctt
    43561 tggcgtcagc gtctggacct ctctggtgcc ggtggttctg atggcgatgc gtgcgattgc
    43621 cgaaatgatc ctgccgaaag gtcacgcttt cctgccggtg gcggagttcc tcggtgaccc
    43681 ggtaatggcc acgctgattg ccgtgctgat tgcgatgttc acctttggtc tgaaccgtgg
    43741 tcgttcaatg gatcagatta acgacacgct gatttcttcc atcaaaatca ttgcgatgat
    43801 gctgttgatt atcggtggtg gcggtgcgtt caagcaggtg ctggtagaca gcggcgtgga
    43861 caaatacatt gcttccatga tgcacgaaac caacatttct ccgctgctga tggcctggtc
    43921 gattgctgcc gtactgcgta tcgcgctggg ttccgcaacc gtcgcggcaa tcactgcggg
    43981 tggtatcgcg gcaccgctga ttgcaacgac gggtgttagc ccggagctga tggttattgc
    44041 ggttggttcc ggtagtgtga ttttctctca cgtgaacgat ccgggcttct ggttgttcaa
    44101 agagtacttt aacctgacta tcggcgagac catcaaatcc tggtcgatgc tggaaaccat
    44161 tatctcggtg tgcggtctgg taggctgtct gctgctgaat atggtgattt gataatactg
    44221 atggccggat gcggcgtgaa cgccttatcc ggcctacgat tcggcattaa ttagtaggcc
    44281 ggataagacg cgtcaagcgt cgcatccggc atcaaccgca ctctacttct tcttcgctgc
    44341 cgctctgcgc cgtctgtcca aatccttcag caatttgttc acaccatcat cggcaaacat
    44401 cgactcaagc gttgcggaaa gtttacgtcg ccagttttta tactggtaac tggtgccggg
    44461 aatatttacc ggatctgcca tatccagcca gtcttccggc tgtagcccta acagggcgct
    44521 gttgctatcg gcgatgtagc gctgtaagcc acgattcagc gtcggtgtca tcgacatcaa
    44581 cgatgcctta tgcccggcac gttttggcag gcaaccatat ttatgcagag catccagcag
    44641 cccttgcttc gccagttcgc gatcctgata cagaccgcgc agtaccactt catccggata
    44701 cagccccagc gttttgccca gcgttaaatc cccactctcc cagtagccgc gtagcgtagg
    44761 caggtcgtgt gtcgccgcaa ccgccatcga ctgctctggg tacgctttcg gtgcgcggaa
    44821 tgtcttctcg tggtcgtttt cgaaatagag cactttgtag gagtacacac cgctgctgcg
    44881 cagcttaccg acaatctcta ccggtacggt accgagatct tcaccaatca ccatacagcg
    44941 atgacgttta ctttcgagtg ccagaatcga gagcagatca tccaccggat agtgaacata
    45001 cgcgccctga tctgccgtct cgccatacgg tatccaccac aaacgcagca tcgacatcac
    45061 gtgatcaatt cgtaatgcgc cgcagttttg catattggca cgcaacagct cgataaacgg
    45121 ttcataggca cgcgcggtga tgatatgcgg gtccattggc ggcaatcccc agttctgccc
    45181 caacgggccg aggatatccg gcggcgcgcc aaccgacgct ttcaggcaat acagttcacg
    45241 gtcgcaccag gtttctgccc caccttctgc tacaccaacg gccagatcac gatacaagcc
    45301 aatcggcatt tcatagccct ggcttatctc ccagcaggcg gcaaactggc tgtaagccag
    45361 ccactgcaac cagagataaa aatcgacgtc atcacgatgt tcttcgcaga actggcgcac
    45421 ttctggtgaa tccacgttct gatacatctc tggccatgct ggccagcccc agcgcatttc
    45481 gtcctctttc acttgctggg catgtagcgc atcaaaggct gcctgccaga acaggctgtc
    45541 gccctgctct gcaacaaact ggcgaaacgc ggtcatctgc tcatcatcac gttgcgcgaa
    45601 acctttccac gccattcgta atgctgtcat ttttagggtg gtaaccgtgg agtaatcaac
    45661 ccagtcggca tcccgcgcct gttgcagcgt ctgttgcgtg gtcggcaact gccaccaggc
    45721 ctgagcctct tcgctaagat ggaaatcttc aactgcgtta acgtcgatat aaatcacatt
    45781 cagccaacgg cgagaagacg ggctgtacgg gctggcactt tccggatttg ccagatagag
    45841 cgcatgaatc gggttcagac caataaacga cccgccacgt tttgccacat ccaccagcat
    45901 cgctttgaga tcgccaaaat ccccaatacc ccagtttttt tccgatcgca gcgtataaag
    45961 ctgaacgcag gcaccccaca gcttttgttt attcagcaac gcctgcggtt cgtaacagcg
    46021 tttcggggcg acaatcaccc ggcaatgcgc gcgctggtcg tcctgggtaa gcgtcagcgt
    46081 gtgataacct tccggcagct tcgtcggtag attgaacgct ttgccccccg tcacatggcc
    46141 tttgtactgc tttccttctt cggtagtcag cagccagcta aattcaccgc taccttcaac
    46201 cgccaccgac atttttttac ccgcggtata aaccatgaca ttcggaactg gcgttaccgc
    46261 cactttcgtg gcggtacgtt gatgcatcgc gtcaagcaaa cgccgtttgg tttcggcgct
    46321 aatcgactgc ggtttaccgt gggcattgat gtaattgggg ctaatccccg ccgccagcgc
    46381 ggcattatcc agacgtttgc tttccatcga acttccttag cgttttgcct gccagatacg
    46441 agcctgataa tcgcgaatag agcgatccga gctaaacata ccgcagcggg cggtattgag
    46501 gatcgccgcg cgagtccagg cctcctggtc gcggtacagc acatccacct gcttttgtgc
    46561 ccccacataa gcggcgaaat ctgccatcac cagatacgga tcgccgccct gtttgccgat
    46621 actgtgcagc atctggtcga aggcatgctt atcgccgtcg ctgtatttac cgctttccag
    46681 ctctttcaat actgcgtcca gcaccttatc tttcttccgc catttcaccg ggtcgtagcc
    46741 tttggccaga attgccttca cttgttccac ggtatgacca aaaataaaga tattttcttc
    46801 accgactttc tcagcgattt caacgttcgc accatccagc gtaccgacag taagcgcacc
    46861 attgagcgcc agtttcatat tgccggtacc ggaagcttct ttacctgcag tcgaaatttg
    46921 ttcggagata tccgccgccg ggatcagttt ttccgccgcc gaaacacaat aatccggcaa
    46981 gaacaccacc ttcaacttgt cgccaaccag cggatcgttg ttgatcacgt cagccacttt
    47041 gttgatcgca aagataatat ttttcgccag gtagtagccc ggtgctgctt tcgcgccgaa
    47101 gaggaagacg cgcggtacgc ggtcagactg cgggttttca cgaatttctt tgtacaacgc
    47161 cagaatatgc agcagattca gatgctggcg tttgtactca tgcagacgtt tgatctgaat
    47221 atcgaaaatc gcctgtggat tgatctcaat accggtacga actttcacaa actctgccag
    47281 acggacttta ttcgcctgct tgatctcgcg atattgctga cggaatttcg catcatcagc
    47341 gaatttttcc agattgatca gttgatcgag atcgttagcc cactcttttt tcagtgattt
    47401 atccaacaga gccgccagtg ccgggttgca ctgtttgatc cagcgacgtg gggtaatacc
    47461 gttggtgaca ttatggaatt tgttcggcca tagctggtga tattccggga acagatcttt
    47521 caccaccaga tccgagtgca gcgccgcaac accgttcacc gcgaaaccgc caaccacaca
    47581 caggttcgcc atatgcactt gtttgtcgtg taccaccgcc agtttggccc acactttttc
    47641 atcgcccggc caggtttttt ccaccagcgt tttaaagcgt gtattaattt cgttaataat
    47701 ctgcatgtgg cgcggcagta agcctttcac cagtttcaca tcccagcgtt ccagcgcttc
    47761 tggcatcagg gtatggttgg tgtaagcgaa agttttgctg gtaatggccc aggcgtcatc
    47821 ccagctcatc tggtgctcat cgatcagcac gcgcagcagt tctggaatcg cgattgttgg
    47881 atgggtatcg ttcagctgaa taacttcgta atccgccagt tcgtgcaatt tacgccccgc
    47941 cagatgatgg cgacgcaaaa tatccgctac cgaacaggca cactggaagt attgctgcat
    48001 caggcgcagc tttttaccgg cagtatggtt gtcgtttgga tagagaactt tggtcagttt
    48061 ttccgcgttg atgccctgct gttcggcacg caggaaatca ccatcgttaa atttagtcag
    48121 atcaaacgga tgcgcatgcg tcgcctgcca cagacgcagc ggctgcgcca cgccattacg
    48181 atagccgaca acggggagat cccacgcttg accggtaatg gtaaattccg gctcccagcg
    48241 tccgtctttc gtcactttac cgccaatccc tacctgcaca tccagtgctt cgttgtggcg
    48301 gaaccacggg tagttaccgc gatgccagtc atccggcgct tcaacctgtt tgccatcgac
    48361 aaaagactgg cggaacaaac catattgata gttcagaccg taacccgtcg cagactgacc
    48421 gacagttacc attgagtcga gaaagcacgc cgccagacgt cccagaccac cgttccccag
    48481 cgccgggtcg atctcttctt ccaacaggtc agtcaggttg atgtcataag ccttcaacga
    48541 atcctgtaca tcctgatacc agccgagatt caacaggttg ttgcccgtca ggcgaccaat
    48601 taaaaactcc attgagatgt agttaacatg tcgctgattc gccaccggct tggcgaatgg
    48661 ctgagcacgc agcatttcgg ccagtgcttc gctcactgcc agccaccact ggcgaggggt
    48721 catttcagcc gcagaattta agccataacg ctgccactga cgtgaaagcg cttcctgaaa
    48781 ttgcttatcg ttaaaaatag gttgtgacat aggagttcca cttttcttag attttcaaca
    48841 caacgttatc gctagtttgc caggctcgat gttgaccttc ctcatcctgc gggggattag
    48901 gcagggagga gttgcgggga tgagcaagga aatgtgatct cgaccactta aagctagtgc
    48961 aaaccacagg attagcagca aatcaatgcc atacagcgca gaaaatctgt atctaagtgc
    49021 aaaaaatggc cgttgcgtat tttcaaaaag cggaaggtaa ctctataaat taagtaaagg
    49081 agtgaaacag tttcacaagt aaaatatcac gtgtgctcca tctcattctt aatagattta
    49141 ttaagatcat ctttttagat ggcactttca tcagaaatga agaagaaacc cttgcttaaa
    49201 tgaaactgat gaacataagg aaaataccgt acaacccagt attcacgctg gatcagcgtc
    49261 gttttaggtg agttgttaat aaacatttgg aattgtgaca cagtgcaaat tcagacacat
    49321 aaaaaaacgt catcgcttgc attagaaagg tttctggccg accttataac cattaattac
    49381 gaagcgcaaa aaaaataata tttcctcatt ttccacagtg aagtgattaa ctatgctgat
    49441 tccgtcaaaa ttaagtcgcc cggttcgact cgaccatacc gtggttcgtg agcgcctgct
    49501 ggctaaactt tccggcgcga acaacttccg gctggcgctg atcacaagtc ctgcgggcta
    49561 cggaaagacc acgctcattt cccagtgggc ggcaggcaaa aacgatatcg gctggtactc
    49621 gctggatgaa ggtgataacc agcaagagcg tttcgccagc tatctcattg ccgccgtgca
    49681 acaggcaacc aacggtcact gcgcgatatg tgagacgatg gcgcaaaaac ggcaatatgc
    49741 cagcctgacg tcactcttcg cccagctttt cattgagctg gcggaatggc atagcccact
    49801 ttatctggtc atcgatgact atcatctgat cactaatcct gtgatccacg agtcaatgcg
    49861 cttctttatt cgccatcaac cagaaaatct cacccttgtg gtgttgtcac gcaaccttcc
    49921 gcaactgggc attgccaatc tgcgtgttcg tgaccagctg ctggaaattg gcagtcagca
    49981 actggcattt acccatcagg aagcgaaaca gttttttgat tgccgtctgt catcgccgat
    50041 tgaagctgca gaaagcagtc ggatttgtga tgatgtttcc ggttgggcga cggcactgca
    50101 gctaatcgcc ctctccgccc ggcagaatac tcactcagcc cataagtcgg cacgccgcct
    50161 tgcgggaatc aatgccagcc atctttcgga ttatctggtc gatgaggttt tggataacgt
    50221 cgatctcgca acgcgccatt ttctgttgaa aagcgccatt ttgcgctcaa tgaacgatgc
    50281 actcatcacc cgtgtgaccg gcgaagaaaa cgggcaaatg cgcctcgaag agattgagcg
    50341 tcaggggctg tttttacagc ggatggatga taccggcgag tggttttgct atcacccact
    50401 gtttggtaac ttcctgcgcc agcgctgcca gtgggaactg gcggcggagc tgccggaaat
    50461 ccaccgtgcc gccgcagaaa gctggatggc ccagggattt cccagcgaag cgattcatca
    50521 tgcgctggcg gcaggcgatg cgctgatgct gcgcgatatt ctgcttaatc acgcctggag
    50581 tttgttcaac catagcgaac tgtcgctgct ggaagagtcg cttaaggccc tgccgtggga
    50641 cagtttgctg gaaaatccgc agttggtgtt attgcaggcg tggctgatgc aaagccagca
    50701 tcgctacggc gaagttaaca ccctgctggc ccgcgctgaa catgaaatca aggacatcag
    50761 agaaggcacc atgcacgcag aatttaacgc tctgcgcgcc caggtggcga ttaacgatgg
    50821 taacccggat gaagcggaac ggctggcaaa actggcactg gaagagctgc cgccgggctg
    50881 gttctatagc cgcattgtgg caacctcggt gctgggtgaa gtgttgcact gcaaaggcga
    50941 attgacccgc tcactggcgt taatgcagca aaccgaacag atggcacgcc agcacgatgt
    51001 ctggcactac gctttgtgga gtttaatcca gcaaagtgaa attctgtttg cccaagggtt
    51061 cctgcaaacc gcgtgggaaa cgcaggaaaa agcgttccag ctgatcaacg agcagcatct
    51121 ggaacagctg ccaatgcatg agtttctggt gcgcattcgt gcgcagctgt tatgggcctg
    51181 ggcacggctg gatgaagccg aagcgtcggc gcgcagcggg attgaagtat tgtcgtctta
    51241 tcagccacag caacagcttc agtgcctggc aatgttgatt caatgctcgc tggcccgtgg
    51301 tgatttagat aacgcccgta gccagctgaa ccgtctggaa aacctgctgg ggaatggcaa
    51361 atatcacagc gactggatct ctaacgccaa caaagtccgg gtgatttact ggcaaatgac
    51421 cagcgataaa gccgccgctg ccaactggtt gcgtcatacg gctaaaccgg agtttgcgaa
    51481 caaccacttc ctgcaaggtc aatggcgcaa cattgcccgc gcacaaatct tgctgggcga
    51541 gtttgaatct gcggaaattg tcctcgaaga actcaatgaa aatgcccgca gtctgcggtt
    51601 gatgagcgat ctcaaccgta acctgttgct gcttaatcaa ctgtactggc aggccggacg
    51661 taaaagtgac gcccagcgtg tgttgctgaa cgcattaaaa ctggcgaatc gcaccggatt
    51721 tatcagtcat tttgtcatcg aaggcgaagc gatggcgcaa caactgcgcc agcttattca
    51781 actcaatacg ctgccggagc tggaacagca tcgcgcacag cgtattctgc gagaaatcaa
    51841 tcaacatcat cggcataaat tcgcccattt cgatgagaat ttcgttgaac gtctgctaaa
    51901 tcatccggaa gtgccagagc tgatccgcac cagcccgttg acccaacgtg aatggcaggt
    51961 cctggggctg atttactccg gttacagcaa tgagcaaatt gcaggagagc tggaagtggc
    52021 ggcaaccacc atcaagacgc atatccgcaa tctgtaccaa aaactcggtg tcgcccaccg
    52081 tcaggccgcg gtacaacacg cgcagaaact gctgaagatg atgggatatg gggtgtgagt
    52141 ttagccggat aatgcgtcgg atccggctta catctttaca tcattcaatg ctaacccgcg
    52201 ttacgccatc tgtttctatc aaaccaaacc gcaccggcaa gaaacgctcc accacggcga
    52261 tattggtcag cagatgacat gagggatggg cgaccgtaaa ttctcccgcg cccgccagcg
    52321 ccatcggtag caccagttgg tcggcgagat attcccccac cgccgccggg cttgccaggt
    52381 agcgtttcac ctctttcacc aactgtgccg cgaccacctc ggcactgacg cgcttttcac
    52441 cgacgacaaa aaagcgttcg gtgatatttt cactttcgac ttcaagcgag acggtattac
    52501 ccggcccctg gtcgcgcggc aggttatgaa tattctgttc atgcaaggaa aaactacccg
    52561 ccagtgtagc gatttcacgc tcagcaacat ggcgcggtaa ctttcgcgaa ctttctgcca
    52621 gcgtcacgcg gatggccacc tttgccacta gcggacgcat cacactggat acagtggaag
    52681 atgagatcaa ccgtctgcgc tataactggc aggagagtcg cccctccacg cttacggcgt
    52741 tgctgggcgc agaggcagaa aacatcgatc tcttcgaccg tatgcaactg gaacacgtta
    52801 tcgctatctg ccgccaggca aagtcgcttt ctgctgccgg acgtgaactg tttgacattt
    52861 cgcgccaggg taaagccagc gtcaatgacg cggatcggct acgcaaatac ctggcgcgtt
    52921 ttggtctgac atgggaagca atgcaggatc agcacagctc cagttgaata tgatggtcct
    52981 tcagcacctg catcacgctc gcaggcggca tcgtgtcggt gtagacagca tcgaccatgc
    53041 tgatgctacc catattgacc attgcgttac ggccaaattt tgagtggtcg acaaccagca
    53101 taacgtggcg cgagttctca atgatggcgc gtttggtgcg aacttcgtgg taatcgaact
    53161 ccagtagcga gccgtcgcta tcgatgccgc ttatccccag aatgccgaaa tcaaggcgga
    53221 actgggagat aaaatcgagc gttgcttcgc caatgatccc gccatcgcgg ctgcgtaatt
    53281 cgccaccggc aagaatgata cggaaatcct cttttaccat cagcgtgtta gcgacgttga
    53341 gattgttggt cacaatgcgt aaattgctgt gattaagcag cgcgtgcgcc accgcttccg
    53401 gcgtggtgcc gatatcgata aacagcgtcg agccattggg aatttgctcc gccactttgc
    53461 gggcgatgcg ctctttttct tcggtctggg tggccttgcg atcgtgccac ggcgtgttaa
    53521 ccgaactgga aggcagcgcc gcaccgccat gatggcgcag gatcaggttt tgctctgcca
    53581 gctcgttgag atcgcggcga atagtctgcg ggctaacgga gaaatgctct accagctctt
    53641 cggtgctgac ataaccctgc tgtttaacca gttcgataat accgttgtga cgttgtgttt
    53701 gtttcattga taaatccctg gaattatttt cgttttcgcg cattgagcga atcaacaaaa
    53761 gccatcgcta aacccacggc taacccggcg atgtgtgctc cgttcgccat cgacatccca
    53821 aacaaatcaa accatccggc gacaatccag atcaacgcaa agataattaa cccacgttgc
    53881 aggtaaatgc cactttgcgg atcgcgttcg ccacgtagcc agacgtagcc catcagcgca
    53941 tacaccacgc cagaaagccc gccaaaccac ggcccgctga atttttgctg cacatagccg
    54001 cttaacaggg cgctaatgag agtaatgaca attagcttac cgctaccgag gcgtttttcc
    54061 accgcaccgc cgagatacca ccaccagagc aggttaaaga ggatatgcat cagcgagaag
    54121 tgcattaacg cgtgggtgaa gtaacgccag aactcaaatt tcagcgtcgg atcgaatggc
    54181 caggccagcc ataacatcac ttcctgatcg ccgagaattt gcatggcgat aaacaccacc
    54241 acgcaggcga tcatcatcac ccaggttacc ggacctgcgc gttcacgcaa ggcggcaaag
    54301 aaaggataac ggcgataatg caggccactg tcggtatgac cggactgcca actggcggcg
    54361 agataacgtg gatctgccgg gttttcgaga aaacgcgcca gctccgcccg cacgcgttcg
    54421 gcctgggact catccgccag ccagacatcg ctttggttat gttgttgaat cgtgaggata
    54481 acaccctgtg tcgccatgta atcaacaaac gcctgcgcca ctcgggggtt agcaaaagag
    54541 gtaatcatca acatcgttgc tgtcgcttat tccacacaaa aggggacagt ataaagcgtt
    54601 acacgccgta cgccacctct gcgggaaact gacgctgcca ggcttcaaaa ccgccatcaa
    54661 tgctgtagac cacatcgtag ccctgttgca gcagatactg cgccgcgcct ttgctgctat
    54721 tgccgtgata acacatcacc atcaccggtg tgtcaaagtc gttatcacgc ataaacgcgc
    54781 ccagtgtgtc gttagttaaa tggaaagcct gggtcgcatg ccccattgcg aaactctgtg
    54841 gatcgcgaat atcgaccagc accgcctctt tttcctgcaa cttctggtgc gcgtcggcaa
    54901 cgttaataca ttcgaactga tccatgcgtc tctctttctt tacaaacaag tgggcaaatt
    54961 taccgcacag tttacgtcga agcggcagat aaacgccata atgttataca tatcactcta
    55021 aaatgttttt tcaatgttac ccaaagcgcg attctttgct aatatgttcg ataacgaaca
    55081 tttgtgagct ttaacgaaag tgaatgaggg cagcatggaa accaaagatc tgattgtgat
    55141 aggtggcggc atcaatggtg ctggtatcgc ggcagacgcc gctggacgcg gtttatccgt
    55201 gctgatgctg gaggcacagg atctcgcttg cgcgacctct tccgccagtt caaaactcat
    55261 tcacggtggt ctgcgctacc ttgagcacta tgaattccga ctggtcagcg aggcgctggc
    55321 tgaacgtgaa gtgctactga aaatggcccc gcatatcgcc ttcccgatgc gttttcgcct
    55381 gccgcatcgt ccgcatctac gcccggcgtg gatgattcgc attggtctgt ttatgtacga
    55441 tcatctgggc aagcgcacca gcctgccggg atcaaccggt ttgcgttttg gcgcaaactc
    55501 agtgttaaaa ccggaaatta agcgcggatt cgaatattct gactgttggg tagacgacgc
    55561 ccgtctggta ctcgccaacg cccagatggt ggtacgtaaa ggcggtgaag tgcttactcg
    55621 tactcgtgcc acgtctgctc gccgcgaaaa cggcctgtgg attgtggaag cggaagatat
    55681 cgataccggc aaaaaatata catggcaggc gcgcggcctg gttaatgcta ccggcccgtg
    55741 ggtgaaacaa tttttcgacg acgggatgca tctgccttcg ccttatggca ttcgcctgat
    55801 caaaggcagc catattgtgg tgccgcgtgt gcatacccag aagcaagcct acattctgca
    55861 aaacgaagat aaacgtattg tcttcgtgat cccgtggatg gacgagtttt ccatcatcgg
    55921 caccaccgat gtggaataca aaggcgatcc aaaagcagtg aagattgaag agagtgaaat
    55981 caattacctg ttgaaagtgt ataacacgca ctttaaaaag cagttaagcc gggacgatat
    56041 cgtctggacc tactccggcg tgcgtccgct atgtgatgat gagtccgact cgccgcaggc
    56101 cattacccgt gattacaccc ttgatatcca tgatgaaaac ggcaaagccc cgctgctgtc
    56161 ggtattcggc ggtaagctga ccacctaccg caaactggcg gagcacgcgc tggaaaaact
    56221 gacgccatat tatcagggca ttggtccggc gtggacgaaa gagagcgtgc tgccgggtgg
    56281 tgccattgaa ggtgaccgtg acgattacgc cgcccgcctg cgccgccgtt acccgttcct
    56341 gaccgaatcg ctggcacgtc actacgcgcg cacttacggc agcaacagcg aactgttgct
    56401 cggcaatgca ggggcgataa gcgacctcgg tgaagatttc ggtcatgagt tctacgaagc
    56461 ggagctgaaa tatctggtcg accacgaatg ggtccgccgc gccgacgatg ccctgtggcg
    56521 tcgcacgaaa caaggcatgt ggctaaatgc ggatcaacaa tctcgtgtga gtcagtggct
    56581 gatggaatat acgcagcaga ggttgtcgct ggcgtcgtaa ggatgatgcc cggttgcgaa
    56641 gtgcaaccgg gctatttgaa ttccttagac tattcattgt aaataattga ataacggccc
    56701 cagagctaaa atcgccaaac ttaagcacgt tacccaatcg ccccacttct tcctatggct
    56761 tctatttttg ctacgccact ggcaataata tcccaacgca aaaccaagct gggcactaat
    56821 cgggccgaca aaatcagtcc acggctgcgg ccaaaaaggg gaaaaattca ggggaagcca
    56881 gacaaatgtc agaaagaaat ttcggatatt ttccagcaac gctgatttct ctggagaaat
    56941 ataaagaatg aaattcgaga gaattaacca gaaatagacc ggagaaaaga ccagccagca
    57001 atgtagcctg ttttttaatt caggaggcgt tctgcgggag aattgccaac cgatgccacc
    57061 cattacactg aaaaaaagtg catattcaac caataactca agttgtaatt cctgaagaga
    57121 aatacgcaca accaggagcg gaaaggagaa tattatttca gccagaaaag ttaacgtgaa
    57181 tataagtacg gctaaggcaa taaccgtcgg ccataccaat aatggtacga taacaaaacc
    57241 gaaaattaca gggtaaatat aaagtctgat tttattcatc tttaatgtcc tgatgattcg
    57301 gatgatgcgt catacatggc acataaacac tcatcaatca tccactcgtc ttcccaggcc
    57361 tgatactgtg tgccctgttc ggactgctta agcggattct caatcgcctg ccgcgcgcgt
    57421 tctttcagga tggattgatt attactagcc caagtattag tttgagtggt aatggcttta
    57481 tcgatatcgt cctgattatc tctgatataa ccgtcacgat acgtattcaa taaacaggca
    57541 agagacgctg ccgaccaaaa tccgggaact gaaataatgg catcaagcaa tggcgaaaga
    57601 gcgatatcgg cacggaaaaa catttccagc ttgtactgta attcgacagc ctcatccccg
    57661 gatacccaat cagtcatata ctggcaagca aattgctgca atatttcccg ttcatcctca
    57721 cgccagtttt ctttactggc aaggttgata cgccgtagag aaaatacttc gtgaatattg
    57781 tcaaattgcc cctgcgcaac aaactccagt aaacaaggca gaaaataacg aacctcatcg
    57841 ctgttttgcg gatcaggtcc tggactacta ttccaggcat tgattaagga ataaggaatc
    57901 gctcgcaacg atgtattccg taatgctttt tgttcctgtt gactcagaca atattcacaa
    57961 caaaccacaa attgctgcgg cgccggataa cgacgaaaaa tatgatatat ctgctcaatt
    58021 aatgcctgca tggcaggagt ggggtgcata attaccccct cgcccgctga tcgtgcataa
    58081 acggctgata gataaaaagg gcattcgccg cgttcaggaa aatattaatg atatccatat
    58141 tgtaatcctg cttaatgcaa cgttttatcc atcggcacat agcccacatc ttccaccaaa
    58201 ctctgcccct gcggcgttaa aaaccactcc accaattttt gtgtctctga cgtcgtattt
    58261 tcccgcgtta ccattaaggc atcgacgata tagggatatt tgtcgttgcg aatattttcc
    58321 gcagtcggtg caataccgtt aatcgctagc agttttatat ttttatcggc attcatttgt
    58381 gttgcgtaat agcggaaggt gtagcctatt gcgttttttg tattacggta ttcggcaaca
    58441 acgttaatca ttccctccat cacgcttgcc acttccgttt cctgcggcga gatcatgcgg
    58501 acatttttca tgacctgcga tagcatcacc gtctggctgc cagagtcttc cgggcgttgc
    58561 caggtctgga tctcctgatc gtcaccgcca accgcgcgcc agttggtaat tgcgccactg
    58621 aaaatgtcac gcacctgttg ttcagtcagg gaattaaccg gattatccgc attgacgatg
    58681 aaaacaaacg cttcacgggc aaatggcgtg tacagcaaag tgacgcccga tttctcagcg
    58741 cgtttttttt gcccatcgga aggttgcgcc acgaaaataa tatcggcatc accgttaacg
    58801 attttgttat atgcttcttg cgtacgagag ttatccagat aatcccaaac gtgaaaatcc
    58861 tctggtatta cacttaatgc ataaaatgca gaggcataaa tggggtacgc cgccgtagcg
    58921 ccatcgagtc gcggccagtt ttgcgtaaat tgaatttgtg gtttaccacg cagtggcgtc
    58981 agttgattat taagtttatc gggtcgccag gcccaggtat caatattttc acgcacaccg
    59041 acaccgggat taagctgttt atataaataa gcctgatacc cagcgacaaa accaagtaag
    59101 agaagtagaa acaggagtaa gttacggcaa cgtagcccgg acgtgccggt taaaggatgc
    59161 cttgtcggcc agccgtatcc cagcgaaaaa ctgatatgcg cgccaacggg tataatgact
    59221 atccaccagt agtcgccact gattaatgag agtaacaaaa caccctgcca ggaaagcata
    59281 aggtttgcca accagcccga tcccatctgg cgaaattgat aatctgccag atgtagcgcg
    59341 attatccagc caaccagggt taatagcgcg ggtaagagaa aaaagcattg tcgccaggcg
    59401 gctatttctc ttttttctgg tgcgctatca ccagcccaca cgcgaccaga gaaaaatatg
    59461 attatcgccc ccagcgtcag cataaagacg ttgagtagaa gataaattgt tggcgtaaag
    59521 agtaaattgc aaacctcaag aatagcggca agcccggccc ccagcatggc aacaaccgct
    59581 gccaaaaatt gcgccagtat ggggatgcca aaaaaagtca ttaccagcga ggtcaaaatc
    59641 cattttccgc tttgcattat tctttccatt cttgctgaat ggcgaaatta tactccccga
    59701 taaccctcgc cccctctgga cacttttcct aaataaaggc gaaaaaaacg ggaccttttg
    59761 gccccgttct gttttttagc gaacttataa tctcaccgga tcaatatgcc aaatataatc
    59821 ggcatactct ttgatcgtac ggtcagaaga gaagtaaccc atattggcaa tgttcagcat
    59881 cgcttttgcg gtccactctt cctgacgctc gtagagttcg tcaactttat cctgacaatc
    59941 gacatagctg cgataatccg ccagtacctg gtagtgatcg ccgaagttga tcagcgaatc
    60001 gaccagatcg cgatagcgac ccggatcttc cggactgaat acaccgctgc cgatttgcgt
    60061 cagcacctga tgcagctcct catctttctc gtagtattca cgcggtttgt agccctgacg
    60121 gcgcaattct tccacttctt ccgctgtgtt accaaagata aagatattgt cagcaccgac
    60181 atgatccagc atctcgacat tcgcaccgtc cagcgtaccg atagtcagcg cgccgttaag
    60241 cgcaaacttc atgttactgg tgccggaagc ttccgtcccc gccagcgaaa tctgttcaga
    60301 cagatcggcc gccggaatga tcaactgcgc caggctaaca ctgtagttcg ggatgaacac
    60361 gactttcagc ttgtcgccaa tctgcggatc gttgttgatc actttcgcta cgtcattgat
    60421 caaatgaata atgtgcttcg ccatgtaata ggccgaagcc gccttaccgc caaaaatatt
    60481 cacgcgcggt acccacttcg catccgggtc ggccttgatg cggttatagc gggtgatcac
    60541 gtgcaacaca ttcatcaatt gacgtttgta ttcgtgaata cgtttgattt gcacatcgaa
    60601 cagcgctttc ggatttacca ccacattcag ctgctgggcg atatactccg ccagacgctt
    60661 tttgttctcc agcttcgcct gatgcaccgc gtgattaacc attgggaaat cacagtgttg
    60721 ttgcagatca ttaagcaggc taaggtcggt gcgccagttg cggcccaggt gttcgtccaa
    60781 cacggctgaa agcgatgggt tcgctaccgc cagccagcga cgcggcgtca caccgttggt
    60841 gacgttggtg aaacgacccg ggaagatttt cgcaaagtcg gcaaatagcg attgcaccat
    60901 caggttagag tgcagttccg atacaccgtt aactttgtgg ctcaccacaa ccgccagcca
    60961 ggccatacgc acacgacgac cattggattc atcaatgatc gacgcccgtc ccagcagatc
    61021 ggtatcgttc ggatactgtt cctgcaaggt tttcaggaaa tagtcgttga tttcaaagat
    61081 gatctgcagg tgacgcggca gaattttacc cagcatatcg actggccagg tttccagcgc
    61141 ctcgctcatt agcgtgtggt tggtgtagga gaagacctga caacacactt caaacgcgtc
    61201 gtcccagcta aattggtgct catcgatcag cagacgcatc atctcaggaa tcgacagcac
    61261 cggatgggta tcgttgaggt gaatcgcaat tttatccgcc aggttatcgt aggttttatg
    61321 caactgataa tggcggctta aaatgtcctg aatggtcgag gaaaccagga agtattcctg
    61381 acgcagtcgc agctcacgcc cggagtaggt ggagtcgtcc ggatacagta cgcgagatac
    61441 gttctcggag tggtttttat cttccactgc cgcgaagtag tcgccctggt tgaatttacc
    61501 gaggttaatt tcactactgg cttgggcact ccacaaacgc aacgtgttgg tcgcgtcggt
    61561 gtcgtaacct gggattatct gatcgtaagc tactcccaga atctcttcgg tttcaatcca
    61621 gcgcgttttt ttaccttcct gctgaatgcg accaccaaaa cggactttat agcgcgtgtt
    61681 gtggcgtttg aattcccacg ggttaccgta ttccagccag tagtcaggcg actctttctg
    61741 gctaccgtta acgatgttct gcttgaacat accgtagtca tagcggatgc cgtaaccgcg
    61801 ccccggcaac cctaacgtcg ccagagaatc gaggaagcaa gccgccagac ggcccaggcc
    61861 accgttaccg aggcccgggt cattttcttc atcaatcagc tcttcgagat ttaatcccat
    61921 cgcttccagt gcgccctgta catcttcgta aattcctagc gacaacatgg cgttggagag
    61981 cgtacggcca atcaaaaact ccatcgacag gtagtaaacc tgacgagttt cttgcgacaa
    62041 ctgggcacgg tttgaacgta accagcgctc cacgagacga tcgcgcacag caaataacgt
    62101 tgcgttcagc cattcatgtt tattggcgac gaccggatcc tttccaatcg taaacatcag
    62161 cttgtaagcg atagagtgct taagagcttc tacgctaagc gtgggcgatg aatatgtaaa
    62221 cggagcattc atataggcgt ttcctgaaaa ctatttcaag cgatagtaaa gctcacggta
    62281 cgacttcgcc gcgacctgcc agctaaaatc cattgccata gcctgacgtt gcacaaaccg
    62341 ccacagtgaa ggacgggacc acagtacgaa agcacgtcga atagcccgta acagcgacca
    62401 ggcattacta tcttcaaaga caaacccact ggcgacgcca tctgcaaggt tctcaagaga
    62461 acagtcagaa accgtatcag caagcccacc ggtgcgccgc actaacggca gcgtaccgta
    62521 cttcaatcca taaagttgcg ttaagccgca cggttcaaaa cggctgggca ccagaatgac
    62581 gtccgcgccg cccataatgc gatgcgaaaa tgcttcgtga tagccaatct gaacgcccac
    62641 ctggccgggg tattccgctg ccgccgcaag gaaaccttcc tgcagcaccg gatcgcccgc
    62701 gccgagtagc gccagctgcc cgccctgctc cagaagacca ggtaaggctt ccagcaccag
    62761 gtcgagacct ttctggctgg tcagacggct caccactgca aaaagcggca ctttatcgtc
    62821 aaccttaagc cccattgcga tttgtaactg gcgcttattt tccgctttat cttccaacgt
    62881 atcgcgggtg taacgcgagg ccaacagtaa gtccgtctct ggactccaga ttttctcgtc
    62941 cacgccgttc agtacgccgg aaagacgccc ttcacggtga cgctgttgca acagaccttc
    63001 cataccgtag gcaaactgcg gttcggtgat ctcgcgagcg taggttgggc tgactgccgt
    63061 aatgtgatca gcatagtaca gaccggcctt caggaaagag atttgtccgt tgaattccag
    63121 cccatgaata ttaaagaatg accatggcaa ttggatgtca ttcatgtgat gtgcataaaa
    63181 catgccttga taggccaggt tgtgcacagt aaacaccgac ttcgccggac gcccgcgcgc
    63241 cgccagatac gcaggcgcaa ggcctgcatg ccagtcgtgc gcatgcacca catcaggacg
    63301 ccagaatggg tcaagcccgc tggccatttc tgccccaacc caccccagca gcgcaaaacg
    63361 caatacgttg tcggtatagg caaataagtt ggtatcgtga tacgggcttc ccggacgatc
    63421 atagagatgc ggcgcgtcaa tcaggtatat gccaaccccg ttgtaatgac cgaacaacag
    63481 cgtgatatgt ccggcgaagg tatcacgacg ggatactacc tgcgcatcgg tcacgccacg
    63541 gcgaatatcg ggaaatgcag gcaacagtac gcgagcgtca acgccgtctg cgatttgtgc
    63601 tgcgggtaat gccccaataa catcagccag accgccggtt ttaagcagcg ggaacatctc
    63661 tgaacataca tgtaaaacct gcattatcgc tcctgtttat gccctaactt ccgtagcatt
    63721 tcgcgcgtta ccagcacgat gccttcttct gaacgataga aacgacgtgc atcttcctct
    63781 gcgttttcac caatcaccat gccttccgga ataacacaag cacgatcgat gacgcagcga
    63841 cgcagacggc acgagcgacc tacccatact tccggtaaca atacggcgga atcaatgttg
    63901 cagaatgaat tcacgcgaac gcgcgagaac agaacggact gcaccaccac cgaaccggag
    63961 atcacacaac cgccggatac cagtgagtta agggtcatcc cgtggctacc ggagcgatcc
    64021 tgcacgaatt tcgctggcgg taatgattca ttgtaggtgc gaattggcca attgcgatcg
    64081 tacatatcca gttccggcac cacagaggcc agatcgaggt tcgctttcca gtaagcttcc
    64141 agcgtaccca catcgcgcca gtacggctcg gcatccgggt cggattgtac gcaagagagc
    64201 gggaacgggt gcgcataggc cagaccggct tcggtgatct tgggaatcaa atctttgcca
    64261 aagtcgtggc tggagttctc atcgcgatcg tcttcttcca gcagttcata cagatagtcg
    64321 gcgtcaaaga cgtagatacc catactcgcc agagatttgc ctggatcgtt cggcattgac
    64381 ggcgggttag ctggtttttc aacgaattcg ataattttat cgttctcatc aaccgccata
    64441 acgccaaacg cggaggcttc ttcaatcggt actggcatac aagcaacggt gcaacgtgcg
    64501 cctttttcga cgtggtcgat aagcatacgc gagtagtctt gcttgtagat atggtcgccc
    64561 gccaggatca ccacgtattc cgctttataa cggcggataa tgtcgaggtt ttgggtgacc
    64621 gcatctgcgg tgccgcgata ccagttttcc cctttcattc tctgctgtgc tggcagcaga
    64681 tcgacaaact cgttcatttc ttcattgaag aatgaccagc cgcgctggat gtgctgcacc
    64741 agagtgtggg actggtactg ggtgatcacg cccatacgac ggatcccgga gttgatgcag
    64801 ttagacagcg caaagtcgat aatgcggaac ttaccgccga agtgtacggc cggttttgct
    64861 cgcttattgg ttaaatcctt caggcgggta ccacgtcctc ccgccagtat cagggcaaca
    64921 gatttcaatg gcagctggcg tgccaacatt aagtgatcgt tcttctctaa actaaccatg
    64981 actaactcct tttttatcat ctctggaaca cacacaatcc gtgtgcaggt ccctgccaga
    65041 cagccgtaat cactgggtta tcctctccag cgaatggggg aatggcgtgc cactccccag
    65101 caggtaaaac aatctctgtt acctcaagcg tggcgttaat tgcgatcaaa aagcgatccg
    65161 agagcagaat ttgcagctgt ttcggcccgt tttgccactc atccgtgctt aaaggttgag
    65221 catatcgatt tagccaacgg acattgccgt cgccttcttc ccaccagcga ttctccatca
    65281 aagcgggaat gcgcttgcgc agatggatta acgcggcggt aaatgcggtt aaaccactgc
    65341 ttgcctgcga ccagtccaac caggttaatt ggttatcctg acagtaggca ttgttattgc
    65401 catgctggct gtgaccatgt tcgtcaccgg ccagtaacat cggcgtaccc tgggagagca
    65461 acaacgttgt taacagggcg tgaatgctgt cgcgccgccg ttcaaccaga tcaagagtac
    65521 cgcctaaccc ttctttacca tgattgttac tgtaattgtt gttggtcccg tcgcgatttt
    65581 cttctccgtt tgcttcattg tgtttatggt tgaagcaaac gcagtcgcga agcgtaaaac
    65641 cgtcatgcgc ggtgacgaga ttaatcgcgg cactcggcag acgatcatta cgtttaaaaa
    65701 catcgctgga ggcagcaaaa cgcccggcaa acgcccccag aggcaaatca taatgcagcc
    65761 agaaacgacg ggctgcatcg cggaaatgat cgttccactc ggcaaacagc ggcgggaaat
    65821 ttcccacctg ataaccacca ggagcgatat cccacggttc agcaattaac ttcacctgcg
    65881 agagcaccgg gcagttctgg atagcggtaa acaacggcgc atcctgacga aactctggcg
    65941 tacggcccat gactgccgcc agatcaaagc ggaaaccatc gacgtggcag gtttctaccc
    66001 aataacgcag gcaggcgctg gcataatcta ccaccgccgg atgactcaaa ttgagcgtgt
    66061 tgccacaacc ggtccagttg tgataatcgc cgtcttctct tatccaataa tagctacggt
    66121 tgtcgatccc acgcagcgag aataacgggc cgtcgaggtc cagttccgca ctatggttga
    66181 gcacgatatc aagaatgact tcgatacccg ctttatgcag tgctttgatt gcatcgcgaa
    66241 actcatccag cgccgtttct ggcgagcagg catacgccgg atgcagcgca aacatcgcca
    66301 ccgggttgta accccagtag ttactaagcc ccatacgttg cagacgtggt tcactggcaa
    66361 attgtgccac cggcagcagt tccagcgcgg taatgcccaa ctgtttcaaa tagttgatca
    66421 tcaccggatg ccccagggct ttataagtgc cacggatctc gaccgggatc tccgggtgca
    66481 ggtacgttaa tcctttgaca tgggcttcat aaatgatggt gctgccccac ggcgtgcgcg
    66541 gcggggcatc atcttcccag tcatagtgat caatcaccac tacgcatttc ggcgcaatgg
    66601 cggcgttgtc gcgatagtca ggttcattat gaccggcgtg cagcagcggg ttatctttaa
    66661 actccccgtc aatttgccgc gcgcaaggat caatcaacaa cttcgccggg ttaaagcgat
    66721 gcccctcggc aggttgccag gggccatgaa ctcgataacc ataacgcaaa cccgggcgcg
    66781 catccggcag ataaccgtgc caaatgtcgc cactgtgccc tggcaagtca tagcgatgtt
    66841 cctggccatt ggcatcaaag acacacagct ctacccgctc ggcatgagcg gagaaaagtg
    66901 tgaagttgac gccctgaccg tcgtaatgcg cgccgagggg agtgggtttg ccaatggcga
    66961 gttgtgtcat tctgcctccc gaaccagcca gatagtggcc agaggtggta gcgtcaggct
    67021 cagtgaatgt tgacgaccgt ggctggcaat ctcatcgctg tgtactgcgc cgccattgcc
    67081 tgcattactg ccgtgatagt gcatggaatc ggtattgagg atttcacgcc atttacccgg
    67141 ctggttaata ccgaagcgat aatcatgacg cggcaccggt gtaaagttac tggcaacgat
    67201 gatttcgtta ccctctttat cgcgacgcac aaagatcagc accgagcgtt ctttgtcatc
    67261 caccaccagc cattcaaagc cgtacgggtc aaaatccagt tcatgcattg ctttatggtg
    67321 gcggtaggtg tggttcagat cgcgcaccag acgctggaca ccgtggtgcc agttatcgcc
    67381 gccttccaac agatgccagt cgaggctggc gtcatggttc cactcgcggc cctgggcaaa
    67441 ttcgttcccc atgaacagta gtttcttgcc cgggaatgcc cacatccagc cgtagtaggc
    67501 gcgcaggtta gcgaatttct gccatgcgtc gcccggcatc cggtcgagaa tcgatttttt
    67561 accgtggacc acttcatcat gcgacaacgg caggacgaag ttttcagtgt agttgtagag
    67621 catcccgaag gtcagtttat cgtgatgata ctgacgataa atcgggtcga gcttcatgta
    67681 gtccagggtg tcatgcatcc agccgaggtt ccacttgtac cagaagccca gaccgcccat
    67741 atcctgtgga cgagaaacgc cagggaaatc ggtagactcc tccgccattg tcactgcacc
    67801 ggaaacctgc tcaccaagaa tacggttggt attacgcaag aattcaatcg cttcaagatt
    67861 ctcgcgaccg ccaaattcgt tcgggatcca ctccccctct ttacggctgt agtcgcgata
    67921 aatcattgac gccaccgcat cgacgcgcag cgcatcaata ccaaaacgtt caatccagta
    67981 aagcgcgtta ccgacaagga agttactgac ttcacggcga ccatagttgt agatcagcgt
    68041 gttccagtcc tgatgatagc cttcgcgcgg atcgctgtgt tcatacaagt tcgtgccatc
    68101 aaattcggca agcgcaaagt catcggtcgg gaagtggcct ggcacccagt cgagaatcac
    68161 gttcagacca gctgcgtgtg cggcatcaat gaaataacgg aagtcgtcgc gagtaccaaa
    68221 acggcgggtc ggtgcataca ggccggttgg ctgataaccc caactgccat cgaagggatg
    68281 ctcgttaatg ggcagtagct cgagatgggt aaagcccatc catttagcat aaggcaccag
    68341 ttgatcggcc agctcgcggt agctcaacca gaaattgttg tcggtgtggc gacgccagga
    68401 gcccaggtga acttcataaa tagagattgg cgcatcaaac tgattcgctt ttttgcgctc
    68461 ttcagtctgc acaacctttt ccggcagccc gcaaataaga gacgcggttt ccgggcgcat
    68521 ttgcgcttcg aaggcataag ggtcggactt cagacgcaag ttgccattgg catcaatcat
    68581 ctcgtattta tagagctgac cgttatgcgc cccagggata aacagttccc agattccgct
    68641 ctctttacgc aggcgcatcg gatgacggcg accgtcccag tagttgaatt gcccaaccac
    68701 cgagacccga cgggcgtttg gagcccagac agaaaaacgc gtacctgtga cgccatccat
    68761 agtatctgca tgcgcgccta aggtttcata cgggcgtagg tgagtacctt cagataatag
    68821 ccaggcatcc atttcctgga ttagcgggcc aaaacggtaa ggatcatcaa tcaggttttg
    68881 ctgaccatgc cagacaacag ccaactgata gcggaaaaaa ttcttacgtc gcggaatgac
    68941 accgctaaag aatccccgtg agtcgagaca ctccagtttt gcgagtttgc gcccggtttt
    69001 cggttcaatc acccacacat cggtagcgtc gggtaaaagg gcacggactt ccagtcccgc
    69061 ggtggtttta tgcatcccca gtacggaaaa aggatccgca aaatggcctg caattagcgc
    69121 gttaatcacg tctctatcga tacgatcgga catgcttgtc ttcctgtttt attgtgtcac
    69181 cccatccaac tgaatttttg acttctggtt gtgacatttt tttgacctga acggcgcagc
    69241 actctgtgca tcctctctgc gtcgtcctca cttcaggtaa ggctgtgaat actcgtgtat
    69301 tccgccaccc ttaaagaata gccaatgctc tatttaactc ccggtaaatc atgaaacatc
    69361 tgcgcttact cctgtattac gcactagcag gggcggcatc gcgccccaga tttaatgaat
    69421 aaagattacg ccagttgacg aagcatccga cgcagcggct ccgcggcccc ccacagcagc
    69481 tggtcgccca cggtaaaggc tgacaggaac tctggtccca tattcagctt acgcagacgg
    69541 cctaccggcg tggtcagcgt gccggtaacg gcagctgggg ttagctcacg catagtgatt
    69601 tcccgatcgt ttggaacaac tttcgcccac ggattgtgcg cagccagcag ttcttccacg
    69661 gtcggaatag acacatcttt tttcaattta atagtgaatg cctggctgtg gcagcgcaat
    69721 gccccgacac gcacacataa accatctacc ggaattacgg aagatgtgtt gaggatcttg
    69781 ttggtttccg cctgcccttt ccactcttcg cggctctgac cgttatcaag ctgtttgtcg
    69841 atccacggaa tcaggctacc cgccagcggc acgccaaagt tatccaccgg cagctcaccg
    69901 ctacgggtta aggttgtgac tttgcgttcg atatcgagaa tagcagagga cgggttcgcg
    69961 agttcatcag ccacatggcc atacagatgg cccatctggg ttaataactc acgcatatgt
    70021 cgcgcaccac cgccggaagc ggcctggtag gttgcaacgg acacccaatc aacaagatca
    70081 ttggcgaata aaccacccag cgacatcaac atcaggctta cggtacagtt accgccaaca
    70141 aaagtcctga tgccattatt taatccgtcg gtaatgacgt cctgattgac ggggtcaaga
    70201 atgatgatgg cgtcatcttt catgcgcaga gatgatgctg cgtcaatcca gtaaccttgc
    70261 catccgcttt cacgaagctt tggatagatt tcgttggtat aatcgccgcc ctgacaggtc
    70321 acaatgatat cgagggcctt tagcgcctcc agatcaaagg catcctgaag tgtgccagtg
    70381 gttccgccaa aagacggcgc agcctgacca agctgagaag tagaaaagaa gacagggcga
    70441 atggcgtcga agtcgcgctc ttcaaccatg cgttgcatga gaacggagcc gaccataccg
    70501 cgccagccga taaaaccaac atttttcata agcgtttttt cctgcaaaga tgtgtgctgt
    70561 ataaatgtgc cggtctcctc ctggcacatc tttcaccata caaaaagcag ccaaagtcgc
    70621 aagtgaaatt aatcaatgat agcgaagcca tcagtaatgc gacttatcct gctttgttag
    70681 cacgcagaaa gtccgcggca attatcaggg aatttgagtt atgaatgaaa tcatttctgc
    70741 aacagtttta ttgatcctga ttatggatcc gctcggaaac ctacctattt tcatgtccgt
    70801 actgaaacat actgaaccga aaagacggcg ggcaatcatg gtgcgagagt tgcttattgc
    70861 tctccttgtg atgctggtgt tcctgtttgc gggcgagaaa attctggcat ttcttagcct
    70921 gcgggcagaa accgtctcca tttctggcgg catcattctg tttctgatcg ccattaagat
    70981 gattttcccc agcgcttcag gaaatagcag cgggcttccg gcaggtgaag agccatttat
    71041 cgtgccgttg gcaattccgt tagtcgccgg gccgactatt ctcgccacgc tgatgttgtt
    71101 gtctcaccag tacccgaatc agatgggaca tctggtgatt gctctgctgc tggcctgggg
    71161 cggcaccttt gtcatcctgc tacagtcttc gctattttta cgtctgctgg gcgagaaagg
    71221 ggtgaacgca cttgaacgcc tgatgggatt gattctggtg atgatggcaa ctcagatgtt
    71281 cctcgacggc attcgaatgt ggatgaaggg gtaaggttca aacaggtgtt gcacacaacc
    71341 agacgcggca cctgttttat tacctgaact gataatgacc ttcgcccaat cggataaccc
    71401 ttccctcttc ttctgcgcgg ttcagccaca cactaatttg ttcgatggtt aaaccagtac
    71461 aagacgccag cctttcaata cttatcgatt cctttgcaag gatcgccagt tccgccacaa
    71521 agagttgata aaaatcgaca ggggtatgcg cgggcattac tgcgtaattt gcgtcatctt
    71581 cgtgcagcga aaacagttct ggttgagcgt tacggagtgc tctgtcatac atcagcccag
    71641 gaacgtctgg cagtctgatc gtcacattct cagcctgttc agcactccat gacgccccat
    71701 tttctactaa tcgtgaattg gctgattgca tatcctgatt tggtttaacc tgcatgggca
    71761 gccactgatg ttttaatgcc tccatcgccc cggttatcgt tccaccggtc attcccgcac
    71821 gtacaaccat tgcgctttca gcaaggcaat aaatatattt attccgcgcc atcgcattgc
    71881 cgacggtgaa acgtacctct gggtaaaacg gcgaaatcaa caccaggttg cctgctataa
    71941 gcccttcacg ccatttcact aacgtactcg tttttaacaa gctatcagct aatacgccaa
    72001 cggcagttcc cccgacctcc agagccgagg ccattgcaca ttcatcaata cctcgcgcac
    72061 caccagagat aacgcaaatc ccctgttggg ccagtttcgc ggccagttgt tgggtatagc
    72121 gcaaatcgtc agtcggagca tctcgcgagc caacaatcgc cataccttcc gcttgcagta
    72181 atgctttatt cccgcagcca aataaaacgg gaggtgcatc cgttcgcaat cggtttttca
    72241 agcgaacggg ataatcggca tctccgcggg ttaagatcca taaaccggcg cgttgccact
    72301 tatctacagc caacgccaga ctatgaccac gcgccagcaa gccaagaatt cgctcctgag
    72361 aaatacgcgg atcgctccag cctgtaagtt tctcttgcgg ttgcggcacc agaagctcgg
    72421 cgggactgat acgttggtgc ttcagccata atgcaaactt cccccattca ctattactaa
    72481 gaggtttata ctcactcgcc gccgcgcgag aaaaatcgct ggttagcagg agagttgctt
    72541 gagcattggc tgaaagattc atcaattttt taccgaggta gaagcaaggg caagaggata
    72601 aaccgtcggg caacctgcct ggcgtaacag tgctgtcaga actgtcagcg tccatgcaga
    72661 atccacgata tcatcaacca gcaacgccgg acctggcatc aaaggagggg taatcacaaa
    72721 cgccccgtcg agattttgac actggtgaaa acggttttgc tgcattttct gcggtggatt
    72781 gtccacgact ttttcaatgg catcaataaa aggtaagcca agtttcgccg ccagtcgccg
    72841 ggcaaaatca ggaaccaggt cgaggtgcct taatgaagga acgcagcata cccaggtggg
    72901 ttcaggatgc ggattccagc gttggcgaac catttccgca catgcctcca ccagctcatc
    72961 actgaagcgc cccgtttttt tctcctgggc tacctgcttt ccccacccgg aatctcccca
    73021 acgagaaagg attcgccccg tagatccttg taaacccgca ggcaagttcc ctttaaatcc
    73081 gtattgcgta aaagccccag atgccacctg tttattgaga tttaatggca ggtcagcgtg
    73141 tttaataaat aaatttgccg catggagtaa gtcgctatca atgtcgggcg acaacagcgg
    73201 atgctgaaga caactgctac atttaccaca acgttcggca cttggctcat cgagtgcgcg
    73261 gcgcagaaat tgcattttgc actccttggt cgtgatatag ctttcaacgt ctgccaactc
    73321 actttttctc tgattcatca aatggttaat tcgttcatga tcaggagaaa aactgacgat
    73381 agttctgcgc cataatttct cggtatacac cacaggcgat ggattttcag ctaccaataa
    73441 tttcagtgct ttttctattt gcccataacg aagattcgtc cgttgttcaa tgcctcgtag
    73501 ggtaagaccg tcattctcgc taagtacgtt gagtatttca tgaatttgcg cctccgcagg
    73561 aaaggcgctt tcacggaaga attgatgaat agcgcggtct tcaccaccac aaagcaatat
    73621 gccaactgcg gaatctatag cacgcccggc acgccccacc tgttggtagt aaccgacaat
    73681 agaccccggc atctgataat gaatgacgaa acctaaatca ggtttatcaa atcccatacc
    73741 taatgccgtc gtcgcgacca gcactttgat tttatttgcc agcagtgcct gctccagata
    73801 ctccctggca gtattagaat cttccgcacc ttcacaagtc acgccgctgt aataagcaaa
    73861 tgcactgata ccatttttcc ttaaccactc ggcgacaagt tcagcatcgc gagtcgtcag
    73921 ggtataaaca attcccgatt tggaaaactg agggataacc gttgctaacc atgccagacg
    73981 ggatgactgt tctcctaaga ctaaggcatc aagcgccaaa ctttcgcgag ccagcgttcc
    74041 acgctgaatc acaatgtcac ccaattgctg acggatatcc tcaacgacac ggttattcgc
    74101 tgtcgcggtt gtacccagaa taggggtatt cgcaggcagt tggcgcaaaa tatctaatat
    74161 acgtcggtaa tccgggcgga aatcgtggcc ccaatcagag atgcagtgcg cctcatcgac
    74221 caccagtaaa ccgatgcggt cagcgactgg atataaaacc gtttcaagaa aatcctgatt
    74281 cgccaaccgt tcaggtgaaa tcagcagaca atctacgcca ccttgcagca acttatcgct
    74341 aatgcgctgc cattcttccc tgtttgtaga attcaacgtt tcagccgtaa tacctaaccg
    74401 ttcagcggcg gcaacctggt tacgcatcag ggcaagcaaa ggagagatga taattgtcgg
    74461 gccagcgccg cgatcgcgga agattttact ggcgatgaag tacacggcac ttttccccca
    74521 gccggtgcgc tgtacaacca gtagttttcg ccgctgatta accaccgcat ctatagcttc
    74581 ccattgccct tccctgaaag tagcgctggt gttacttaac atccgctgta acagcaattc
    74641 agctccgtgt ttctccatca tcttctccct cgtccgttaa cacagtgatt agaacggata
    74701 atgttccggt ctttttgcga gagagatcaa aaagatgaag acaggagaaa agagaaatga
    74761 attactgcga gcaagctcgc agtaatttgt cgcagtaatt aactcaacag ctggaacgca
    74821 atcatcccaa cgatggcacc gacagtgccg aggatggttt ccatcatggt ccaggtttta
    74881 agcgtttcgg cttcggtcgc gccggtaaat ttaccgaaca accagaaacc cgcgtcgtta
    74941 acgtggctga caacaatcga accaccggcg atacaaatcg acagtgccgc catttgcgca
    75001 ccggagtagt tcagttgttc aatgaccggc attaccaatc ccaccgccgt taaacaggca
    75061 acggtagcag aaccctgaat gatgcgcact gcagctgcca gcacgaagca ggtgatggca
    75121 atcggcaggc ccatgccggt taacgcttcg cccagtgccg gaccgacacc agagtcaacc
    75181 agcacctgtt tgaacacgcc gcccgcacca atcaccagca gaatgatccc cgccggttgc
    75241 agcgcgtgac cgcaaatctc catcactttg tcttttggca taccctgacg cattgccaga
    75301 ccgtaaatcg ccaccaggca agcaaccaga atcgcggtaa acggatgacc aataaactcg
    75361 aaccattcgt aagcggtaga accttctggc acaaaacgcg cggcaatggt tttcagcccc
    75421 accagcacca gtggcaacag gatcagcgac aggctgaatc cgaaggacgg cattttgcct
    75481 tcgccgagat gcggttcgct gatgtcgtca ggaatatgca actccacgta gcggctgatg
    75541 aaattacccc acagcggccc ggcaataatc attcccggaa ttgccgcgca caggccaatc
    75601 aggatcatcc agccaaagtc ggcgttcatc tgcgatgcca gcagcattgg cgctggccca
    75661 ggcaccagga acgcagccgc tgccgccacg ccggcgaata atgggattac cagcttcacc
    75721 aggttcgtgc cggtgtggcg cgccattgag aaagcaacgc taatcagcag aacaatcgcc
    75781 acttcaaaga acagcggtag cgcacaaacc agccccgcaa ggccgatggc ataatgcacg
    75841 cggctgtgac cgaaggattt gagcattttg acggcaatct gatcgaccgc gccggtttca
    75901 tgtaagatct taccaaacat ggctcccagg gcgacaacca ccgccaggaa gccgagggtg
    75961 cctcccatcc ctttttccat cgtcgctgcg attttatcga gcggcatacc ggaaaaaagg
    76021 ccagccccca tggacaccac cattaaagcc aggaaagcgt gcatacgcgc cttcatgact
    76081 aaaaacagca gcagtaaaac agaccctact gctgttaaaa caagcgttaa tgtagtcact
    76141 acttatttgc cttttttaat aacctcaatg gtgcttgcca caacaccttc cagcggttga
    76201 tcaatatcca ccaccagtac atcggtttcg tccgcacccg gctcctgcag cgtttcaaac
    76261 tgcgtcacca acatttgggt tttaaagaaa tggcctttgc gcgctttcag gcggctttca
    76321 atcacatcaa aatcgccttt caggtagatg aaagagagat taggattacc ttcacgcagc
    76381 aagtcgcgat agtgtttttt cagtgcagaa cagacgatca gcgatacttt attggtgcgc
    76441 tgcatagcaa acgcggcgtc gttcagcgcc tgcaaccacg gtttgcgatc gtcgtcattc
    76501 agcggttcgc cagacgccat tttttcgata ttgcagcgcg gatggaggaa atcgccatca
    76561 agaaacgcgg catgaagttg atgcgccact tcactggcga ccgcagattt gccgctgccc
    76621 gatacgccca tcaagacgta aatgtggtga tcatggttag tcgtgctcaa agtggtgccc
    76681 ccacaataca agaattaaaa atgttacggg taactgttat cggtaacatt gtccagccgg
    76741 acaatatcag aagcaatatc catccgtgcc ttaagtgtat aagtgtgagc tacttcaaat
    76801 ttgtaggctt aaatagatcc gcccggtgac aaggtgaaac ctaaatctaa cattttcggt
    76861 gtcacagatt cgccacgaat acgcgccagc aggcgctcag cgccaatgct gcccatccgc
    76921 tcacgcggcg tcagcacact cgccagccgt ggctccatca cctgaccaat gtcatgaccg
    76981 tggaaaccgg caatcgccat atcgtcagga actttcaacc ccagacgctg acattcaaac
    77041 gccgcgccga ccgccaggtc atcattagta cagaacacgc catccagctg cggatattcc
    77101 cgccgcgcct ggcgaatcag ttcaataccg gaagagtaag aagaagattg ctcaaccatc
    77161 acgctatacg gcaccaggcc tgcatccagc atcgcctgtt cgtatccctt ctgtttgatg
    77221 atagtacgtt cgtcgagacg tgcgccgaga taggcaatgt ggcgatgccc gcgagcaata
    77281 atggcggtgg tcatctggcg tgccgcttca aagttatcaa aaccgacggc gatatcgaga
    77341 cacggcgact tactgtccat caactccacc accggaatcc ccgccacttc aatcatcttt
    77401 aaggtgcgtg gcgtgtgggt acgttcagtg aggatcaggc cgtcgatatt ccatgagagc
    77461 attgattcga ggcgttcttg ctccatttcc ggtttataac cgtagtgcgc cagcatggtt
    77521 tgataaccgt gcgcgtcggt gacactttcg attccgcgta atacttccgc gaaaacctgg
    77581 ttggtgagag aaggtaacag gacgccaatc gcccggctgg tggcgttaga gagtatatcg
    77641 ggcgcgcgat tgggaatata acccagttca tcaagagcgg cggcaatctt gccgcgtaga
    77701 gcgacggaaa cctgctccgg gttgcgtaaa aaacggctga ccgtcatttt ggtcacgcct
    77761 acacggtcag ccacatcctg aagtacgggt cttttctttt tcatcgtcct gaaggtacaa
    77821 aagagataga ttctcttagt ttaacacgga cgcatcacaa ccttcctgat tcctcaggaa
    77881 ggtttgtgag ataaatttag actggcggta aatcgaacag caggatttca ctgtcgctat
    77941 cagcatggat ggaaatcgcc tgctcatccc agattgccag accatcgctg gtcgaagctt
    78001 ttacgccgtt aatggtgaca ttgcctttca ccacctggat ccagatgcgg cgttcagcgg
    78061 caatctgatg caccgactgc tcatctttca ccagcgccca acgatacagt tccatatcct
    78121 gatgcacttt caatgaacca tctcgcgcat ccggcgaaag caccagttgt ttgccctgca
    78181 cggcatcgaa gcgacgctgt tcataacgcg gcgtaatacc gttttcttca ggcatgatcc
    78241 agatctgata cagatgcaga cgctcggtgc tgcttgggtt gtactctgag tgacgaatac
    78301 ccgtacccgc actcataatc tggaactcgc ccgccggaac ctgctcttta ttgcccatgc
    78361 tgtcctggtg ctcaacagta ccttccagca cgtaggtcaa aatttccata tctttatgcg
    78421 ggtgagtgcc gaagccctgc cctgcttcaa taacgtcgtc gttaatcacg cgcagcgcag
    78481 agaagcccat aaagttcgga tcgtaatagt tggcaaaaga gaaagtatgc caggagtcca
    78541 gccagccatg atttgcatga ccgcgttcat ttgctttgcg taagtagatc atttgtattc
    78601 gccccctgaa tgatttcgat gggcttagtg tggacccgat ccgcctggga tgatagaggg
    78661 tgaaaattga cccctctgtt caaaaaaatt gaactattcg aggggccatc aaacttacct
    78721 cgcgagactc actgcggagg gagaagcttg ctcaaaaccg cgttcaagga tttccagatt
    78781 cgtaagaact tcagattcct tgacgtaatt tggcgcaccg ttggtaatgg tttgatacaa
    78841 cgcatcataa acgcgcccat aatcgcccat ctctggcttc atctcttctc ttaccgtcac
    78901 gccctcgtca ttgacatact ccagcacacc gaccgaatca tccgctgcga atcctggttc
    78961 gcccggcata atattagcct tcaggctggt ttcctgctgg tcgataccgt atttaataaa
    79021 cgaacctttc ttaccgtgaa cgataaattt cggataatcg attttcacca gatggctggt
    79081 tttgacgatg gcttttagat cgccataaaa cagctgcgct tcaaaggtgt cgtccggatt
    79141 ggctttatta cgcaggctgc ggatgtcata agcgacgtga tccgggcgac cgaacagaga
    79201 aataatctgg tccatcgtat gcacaccaag gccatagaac gcgccatcct gcggcagccc
    79261 cggtttggtt tctgccaccg ggcggtaata gtcaaaatgg ctttccactt cgacaatctc
    79321 tcccagcttg ccgctttcaa tcgctttttt cgctgtcagg aagcaggaat caaagcgacg
    79381 attctgatac ggcgtaacga tcagcccttt gcttttcgcc agtgcaaaca gctctttcgc
    79441 ctgcgcaatt gtcggagtga acggtttttc gaccagcaca tttttcccgg cttccagcgc
    79501 gcgcttcgcg tactcgaagt ggctgtccgc gtgggtgcag acgacaacca gcttaacatc
    79561 gggatcgttt agcacttcgt cgagatcgct ggtgaaatgg atatgggaat aaatgggggc
    79621 ctgttcttcc ggcttcgcat ggcggcgaaa aatatgcgcg acatgccagc tatccttgcg
    79681 gttaagtaca tacggcagat ggtaacgggt ggtgcttttg ccgaagccaa taaaggcgca
    79741 gttaatgacc atgatttcgt cctttttaag gtggttattt acaccttagc gcaaagcgga
    79801 cgtggttcct actggagtgc gcataacgaa cacaagcact tcccgaggat aaattgagaa
    79861 cgaaagatca aaaaaaaagc cagcacccgg ctggctaagt aatactggaa gcaatgtgag
    79921 caatgtcgtg ctttcaggtt ctccgcgagg gtcttcctga tcgcgagaca ataataatca
    79981 ttctcattcg cacttgtcca acactttttg caaaaaaatg catttgactc gcatttgaaa
    80041 gtcaatgatg ttgaaaggga catttacccc aaagaggaca aaggaatgag tgagatagta
    80101 atacgccacg cagaaacacg cgattacgag gccatcaggc agattcacgc ccagccggag
    80161 gtgtattaca acacgctaca ggtgcctcat ccttcagaac aaatgtggct ggaacgactc
    80221 accgctcgcc ctggcatcaa gcagttagtc gcctgtattg atggaatcgt cgtaggccat
    80281 ctcaccattg acgtgcaaca acgcccacgc cgcagtcatg ttgccgattt tggtatctgt
    80341 gtcgactccc gttggaagaa ccgcggcgtc gccagcgccc tgatgcgaga gatgattgaa
    80401 atgtgcgaca actggttgcg ggtagatcgt atcgagctaa cggtgttcgt cgataacgcg
    80461 ccagcaatta aggtctataa aaaatttggc tttgagattg aagggactgg caagaagtac
    80521 gcattacgaa acggtgaata tgtcgatgcg tattatatgg cgcgtgtgaa atagagaatt
    80581 tgcaatctac gtttgtcatt taagccctca cctaccggga gagggcttaa gatattaata
    80641 ccccgccgtt aaatcatcca ccgagcgcgg gtcggatgca ccgtacagct caccgtccgg
    80701 cccaaccata atgctttgtg tactgcccat cgcctctttc agcgccactt tctgaccttt
    80761 tgcttccagc agcttgatcg tatccgggct aaaccctttt tcgacacgca gctcgtccgg
    80821 caaccactga tggtggaaac gcggcgcatt ggtcgcttcg gcgacgttca ttccataatc
    80881 gatgctattc accaccattt gcagcacagt ggtgataatc cggctaccgc ctgggcttcc
    80941 ggtgaccagc caggttttac catccttgac cacaatggtt ggtgacatcg acgacagcgg
    81001 gcgtttattc ggcccgacgg cgttggcatc gccgcccacc agcccgtaaa cgttcggtac
    81061 gcttggtttg gcggagaaat catccatctg gttattgagc aaaataccgc tctcgcccgc
    81121 gacaatgccc gtaccgaagg tggtgttcag tgtataggtc accgccacgg cgttaccgtc
    81181 tttatccact actgagtaat gggtagtttg attactctca taaggcgcaa gcttgccagg
    81241 acgaatttcg ctggatggct tcgctttatt gatatcgatt tgatcggcaa tagatttggc
    81301 ataggcttta ttggtcagcg cctgccacgg tactttgaca aaatccgggt cgccaagata
    81361 ttccgagcgg tcggcgtagg cgtatttctc agcttctgcc atgatttgca tcgcgtcggc
    81421 gctgccaaag ccgtacttct gcatatcgaa gttttccaga atattgagga tttgtacgat
    81481 atgaataccg ccggaagatg gcggtggcat ggagtaaacc tgatacccgc gataatcgcc
    81541 gcttatcggt gtgcgttcga ccgctttata tgccgctaaa tcttctttgc tgatcaagcc
    81601 accgtttttc tgcatctcct gggcaatctg ttccgctatc gtgcctttgt agaattcatc
    81661 cgggccgttt tcggcaatca tctccaggct ctttgccagg ttcgcctgca ccagcttgtc
    81721 gccctttttc agcggttcgc cctctttcca gaagatagct ttgctgtttt cgtggttcgg
    81781 cagcacttca ctgccgtagg ttttgagatc gtcagccagc gcgtcgttaa cgataaaacc
    81841 atcgcgtgcc agtttaaacg ccggttgcac cactttattc aacggcatgg tgccgtattt
    81901 atccagcgcc aacgagaaac ccgctaccgt accgggtgtg ccggaagcca gatgcgaagt
    81961 gagtgatttt ttgctgtccg tgttgccctg atcatcgagg aacatatcgc gggtcgcttt
    82021 ggcgggtgcc atttcgcgga aatcgatagc cgtggtattg ccatttttcg agcggatcag
    82081 cataaaacca ccgccgccca gattccctgc ctgaggatgc gttaccgcca gcgcgtagcc
    82141 caccgccacg gcagcatcaa cggcattccc gccctccttg aggatatcca ctcccacctg
    82201 agtggcagta acatctacag acgccaccat cccttgtttc gcgcgtaccg gatggaagac
    82261 atcttcctcc acaccataag aaacaggagg cgcggctacc gtactaaaac aacttcctgt
    82321 gagcagagca gcaatggcca cccggcgtaa aaacgtcggt tttatcatcg ttattctcca
    82381 gagattaagg ggcaacccca gctaagcctg gtatataact ctgaattaat catcgttttg
    82441 ccgggaagcg agtaaactta aagaatatct tcagaggagg atacgcgatg aaacgacttc
    82501 tgcttttgac ggcactcctg ccgtttgtcg gctttgcaca gcccattaat actctgaaca
    82561 accctaacca gccgggatat cagatcccca gccagcagcg gatgcaaact caaatgcaga
    82621 cgcagcaaat tcagcaaaaa gggatgctga accagcaact gaaaacgcaa acgcagctgc
    82681 aacaacagca tttagaaaac cagataaaca ataattctca gcgggtgttg cagtcacaac
    82741 cgggggagcg aaatcccgcc cggcagcaaa tgctgcccaa caccaacggc gggatgttaa
    82801 acagcaaccg taatccggat agttcgttga atcagcagca tatgctgccg gagaggagaa
    82861 acggcgacat gctgaatcag cccagcacgc cgcagcctga cattccgttg aaaactattg
    82921 ggccgtaaag ttcggaccaa tcacgtcaat cgcatcggta cagatgcaat ccacgcccca
    82981 gcgcagcaac tctgccgcgc gctggggttt attgacggta taaaccagaa ttcgcagtcc
    83041 tgcatctttc aactgcatca ctcgcgcttt atcgagcaac ttataattga gatgaataga
    83101 gacgcagccc agccgcgcgg tcaattctcg ccagtcgtcg cgccattcat caagcaataa
    83161 accacgcggc agttccggtg ctgcctgttg agcggcttct aaagcatcaa tctcaaacga
    83221 tgacagcagc ggcggcgtca taccggtcca cagttcgcgt gccgccaacg ccaccatttt
    83281 gcccgttaat ggtccagtgt cagtggtggg tttgatttcg atattcgcca tcatcccgtg
    83341 tttgcgacag cgttccgcca cctgcgaaag caacggcagt ggctcacctt taaacgcttt
    83401 gctgtaccaa ctgcctgcat ccacgcgcag taaatcctgc cagttcagtt cacccgcgac
    83461 gccccagccg ttgctggtgc gttcaagatt gtcgtcatgg agcaggaaga tctcgccatc
    83521 tttcgataac ttcgcgtcaa attcgatcat cttatgaccg tattttgccc cgacgtcgat
    83581 tgctgccagg gtgttctccg gggccagttt accgccgcca cgatgggcga cgatgcgggg
    83641 ataaggccag ttactcatac tcgttgtcct gtttcaccat caaaaagatg tagctgattt
    83701 tccggcagat gcagccacag cgtgctgcct gccgtcgggc gctcctgatg tgccagtcgc
    83761 accaccagct tctgttcgcc ccaatgtccg tgcgccaggt tatctgcgcc gaggatctcc
    83821 agcgtgtcca tcaccagcgg cacaccacct tctgcctgcg agcttagcgc tatatgttcc
    83881 gggcgaatgc cgagggtcat tttacgcccg acatactgac ggtagccacc gtttagcggc
    83941 agcgcaatac cgccatccag ttcgaaatgc gtaccttcgt tattcacacg gccagccagt
    84001 aggttcatcg ccgggctgcc gataaaactc gccacaaaca ggctggcggg cttttcgtac
    84061 acttcaactg gcgtgccaat ctgttcggca acgccgccgt tcatcaccat tactcgctgg
    84121 gcgagcgtca tcgcctcaac ctgatcgtgg gtaacgtaga gtgaagtcgt tttcaggcga
    84181 cggtgcagct gttgcagttc aagacgcatc tgcacacgca acttggcatc gaggttagag
    84241 agcggctcat caaataggaa cactgccggc tcacgcacaa tcgcacgccc catcgccaca
    84301 cgctgacgct gaccgccaga tagctcacgc gggcggcgct taagcaggcc gtccagctcc
    84361 agaatgcgcg ccgcttcttt aacacgttcg gcaatttgtt gcttgcccat gccgcgaatt
    84421 ttcagccccc acgccatgtt ctcctctacg ctcatatgcg gatagagcgc gtagttctgg
    84481 aacaccatcg caatcccgcg atctttcggc tccatttcgg tgacacgctt gcggtcgatc
    84541 cagatatcgc ccgtcgttac ccgctccagt ccggccacca ttcgcaataa cgtcgatttc
    84601 ccgcagccag acggcccgac catcacgata aattcgccat ccgccacatc aagggtcagc
    84661 ggtttaatga cctgggtttt gccatcccag cttttggtta ctgcctgtaa tttcagtcct
    84721 gccatcttat ttctcactat cgaccaggcc gcgcacgaag gcacgctgca tcactaaaac
    84781 aatcactact ggtgggatca gggttaacaa catcgccgcc atcaccgagt tccattccgt
    84841 tgtgccttcg cctgtggcga tcatcccttt aatccctgcc acggtggtgc cgagatcaac
    84901 gtcggtaata atcaacaacg gccacaaata ctggttccag ccgtaaataa aggtgatcac
    84961 aaacagcgcc gccagattgg ttttggacag cggaaaaacg atgtcgcaaa agaaacgcat
    85021 tggcgacgcg ccgtcgatcc gcgcggcttc caccagttca tccggtaggg tcataaagaa
    85081 ctggcgaaac agaaacgtgg cggtcgccga ggccatcagc ggcagcgtta aacctgcgta
    85141 gctgtcgagc attttcaggt tggcgatgac ttccaccgtc gggaagatac gcacttcaac
    85201 cggcagcatc agggtgataa aaatcatcca gaagaagagg ttacgtagcg gaaaacgaaa
    85261 ccagacaatg gcaaatgccg agagcatcga gacggtaatt ttgccgagcg taatgctgaa
    85321 cgccatcaca aagctgttaa gcaacatccg ccagaacggc gcgctattcg tgcctacccc
    85381 gtttacccag atgttgtgga tgttttccag cagatgtgtg ccgggaataa gcgtcatcgg
    85441 cgcggcgtag acttcttgtt tatccagcgt cgccgcgaca aacgccacgt acagcgggaa
    85501 gaggatcacc gcgatcccga ggatcagcat ggtatggctg aatattgtca gccacggacg
    85561 gttctcaatc attggtaacg taccttgctt tcaacatagc ggaactgcac caccgtcagc
    85621 acgatgacga ggaacatcaa caccaccgac tgcgcggcag acgaagccaa atccagtccg
    85681 gtaaagcctt cacggtagat cttataaatc agcgtcgtgg tggcctgtac tggcccgccg
    85741 gacgtggcgg cgtcgatcac cgggaaggtg tcgaagaagg catacaccag gttcaccacc
    85801 agcaggaaga aactcaccgg agcgataagc ggcagcgcaa tcttaaagaa gcggcgaatc
    85861 gggccagccc catcaatggc tgcggcttcg atcaacgaac gggggatgga ttgcagcgcg
    85921 gcatagaaga acagaaagtt gtagctgatt tgcttccaca ctgaggcaaa caccaccagg
    85981 aacatcgcct gaccgctgtt ttgcgcgtgg ttccagtcgt agccgaactc agcgagaaaa
    86041 tgggtgatca gcccgcgacc ggggttaaac agaaagatcc acaatacggc ggcaacggcg
    86101 ggagccacgg catacggtag caacatcaag gtttgataga aacgactgcc gcgcacgatg
    86161 tactccacca gcgctgcgaa gaacagcgat accagcaaac cgctgacggt gacaaaggtg
    86221 ctgaatttta tcgtcgtcca gaaggcgtcg agatagtagc tgtcgtgaaa cagcgtgacg
    86281 aaattatcca ggccgacaaa ctggctggag aaaccaaacg gatcgacgct ttgtagcgag
    86341 taccacaacg cttcgcccgc aggccagata aaaaagataa cggtgatgat gagctgcggc
    86401 gcgaccagca aataaggtag ccagcgcgag cggaacaccg gacgggatga tggcattact
    86461 gttaattcct gaactgtgcc ggatgcgctt cgcttatccg gcctacacgc gatgccaggt
    86521 cagataagcg aaacgcatcc gacattacac tgattaagac ttcgtcgatt tctcaaagcg
    86581 gcgcagtaac tggttcccac gctcaacggc agtatccagc gcctgctgtg gtgtcttctt
    86641 accggtccac acgctctcca gctcttcatc cacaatcacg cggatctgcg gcatgttgcc
    86701 cagacgcaaa cctttggtga acggcaacgg tggcttgttc agcatctgac gcgtggcaat
    86761 atccgctcct gggtttttct cgtaaaagcc ctgctcacgg gtcaggtcat acgccgcttt
    86821 agtgattggt agataaccgg ttttctgatg ccactcggca gcgttttctg gttttgcgag
    86881 gaagtcgagg aacttcgcca cgccggtgta agtttcttta tctttaccct gcatcaccca
    86941 caggctggcc ccgccgataa tggcgttttg cggcgcgtct ttcgcatcgg cgtcgtaagg
    87001 catcatgcct acgccatagt taaatttggc gtactcgcga atgttggcaa gagaaccgga
    87061 agaggcggtc gtcatcgcgc aatcaccgtt atagaacttc tcggtggatt catccttacg
    87121 cccaacgtag ctgaaatcgc ccttcttgtt catctcttcg agcatggcga tgtgtttcac
    87181 ctgctccggc ttgttgaact caagcaccgc gtctgtgccg tcaaagccgt tgtttttgct
    87241 ggcaaacggc agaccgttcc aggcgctaaa gttttccagt tggatccagc cctgccagcc
    87301 gctggcgtag ccgcacttca tgccggaggc ttttagtttt gcggcatagt ccgccagatc
    87361 ctgccaggtt tttggcggct gttccgggtc taatcctgct ttcttgaagg cgtctttgtt
    87421 gtaatagaga acgggggtcg aactgttgaa cggctgggaa agtaagtgcc cagttttgct
    87481 gtcggagtag taaccggaaa ccgtcggcac aaactgcgac tcatcgaatt gaatccctgc
    87541 ctctttaaat acgtcataca ccggtttaat ggctttcgac gccatcatgg tggcggtgcc
    87601 aacttcataa acctgcaaaa tagccggagc gttgccggta cgaaatgcgg caatccccgc
    87661 gcttaaattc tgttcgtaat tgcctttata ggtcggtaca attttgtaat ccgggttttc
    87721 ggcgttaaaa cgttgggcca gagaatccac ctctttaccc agttcccctt ccatagaatg
    87781 ccagaacgga atggtcgtca ctgcctgcgc atttgccatt aaagccagtc cgagcgccag
    87841 tgctgaagct gtataacgta acggtttcat tgattatctc tcttgttgta ccgaatgcgc
    87901 gaattcacgc gttttatgct cgcggagtaa catgacatgc tcgaattaca gaaaaataac
    87961 tattttgtta catttttaag atagtaaggt gtcagaaaga tgacaaggcg gtgacggcgc
    88021 gggcgaggga aaatgggaga tggggcacgg ataagcggaa aaatagaagg tctgaatcaa
    88081 actctacaga ttactcatcg tttcatgccg gatgcggcgt aaacgcctta tccggcctac
    88141 aagatcgtgc aaattcaata tattgcaact cacccagtag gcctgataag cgcagcgcat
    88201 caggcaattt tacatttgtc acctgtctca aaggagtctt ttgactcctt atcaatcaac
    88261 gtgttattac ccgcctaaat atgcacttct cactgcttca ttcgccagca gcgcgtcgcc
    88321 agtatcggag agcactacat gaccgttttc cagcacgtag ccgcgatcag ccagctttag
    88381 cgcctggttg gcgttctgct cgacgaggaa gatggtcatc ccctgctcgc gcagttgttc
    88441 gatggtgtcg aaaatttgct ggatgataat cggtgccagc ccgagcgatg gctcatcaag
    88501 cagtagcaaa cgcgggttac tcatcaaagc gcgaccaatc gccagcatct gctgttcacc
    88561 gccagacata gtgcccgctc gctgaacacg gcgctcatgc agacgcggaa acagttcata
    88621 gacccacttt atgcgctcct ggaactggtc gcgttcggca aaaaagccgc ccatcgccag
    88681 gttctcttcc accgtcatcc gcgagaagac gcgacgccct tccgggacaa tcgctaccgc
    88741 ttcgcgcatg attttcgccg tctgccagtc ggtaatgtct ttatcatcaa acacaattcg
    88801 cccgctggtg gcgcgcggat cgccgcataa cgtgccgagc aaggtggttt tccccgcccc
    88861 gttcgcgcca atgagggtga caatctcgcc ctgattgata tgcaggctca cctcatgcag
    88921 cgcctggatt ttgccgtagt gggcgctgac tttgtcaaag gacaacatga ctttttccat
    88981 cttatgcctc acctaaatag gcacggatca catctgggtt attacggatc tgctccggtg
    89041 taccgtttgc cagcggcgtc ccctgattga ccacgtaaat tctgtctgaa atccccatca
    89101 ccagcttcat atcgtgttca atcaacaaga tagtggtgtt gtgatggtta cgcagttcgg
    89161 caatcagctc atccagctct ttcgtctctt tcgggttaag acctgctgcc ggttcgtcga
    89221 gcattaaaat ctccggctgc gtcaccatac agcgggcaat ctccagacgg cgctggtcac
    89281 cataggccag gttactcgcc tgacggttgg catgttccag caaaccaatg cgctcaagcc
    89341 aggtggcggc gcggtcgagc gcttcgctct gggcgcgacg gaaagagggg gttttcaaca
    89401 ggccggagaa cagcccagtt ttcagttgct gatgctgtgc caccagcagg ttttcaatca
    89461 ccgtcatttc acggaacaga cgtacatgct ggaaggtgcg caccacgccc atgcgggcaa
    89521 tttgctgccc cggtaaacct tccaggtgct gatcgcgcag taaaatggtg ccgccggtgg
    89581 gtttatagaa tccggtcagg cagttgaaga ccgtggtttt tccggcaccg ttagggccga
    89641 ttaacgagac gatctcctgc gggtacagtt caagattgac gttgttcacc gccagcaggc
    89701 cgccgaagcg catcatcagg ccgttaacag ataataatgg ctgactcatg cctgctctcc
    89761 ttttgctgcg ccgtttttca gcttgagttg cgggcgcgtc atgggcagca agccctgcgg
    89821 acgccagatc atcatcagca ccatcaaacc accgagcatt aacatgctgt attcgttgaa
    89881 atcacgcatc aactcgcgcg ataccaccag caaaacagcc gcaagaatca ctgcaaattg
    89941 cgagcccata ccgccgagca ccactatcgc cagcacaaac gccgattcgg caaaagtgaa
    90001 ggattcaggg ctgacaaagc cctggcgcgc agcaaacaac gttccggcaa aaccggcaaa
    90061 cgcagcgctg atggtaaagg cagtcagctt gatacgacgc gggcttaagc ccagcgaacg
    90121 acaagcgatt tcatcttcac gcaacgcttc ccacgcacgc cccagcggca tccgcagcag
    90181 gcggttaatg acaaacaggc ttaacaccac tagcagcaac gccaccaggt agaggaagat
    90241 gacgcgatcg gagggatcgt atttcaggcc aaagaaattg ctgaacgtgt cccagccgcc
    90301 ttcgcgggcg gtacggctga actcgagtcc gaagagcgtc ggtttcggaa tctgactgat
    90361 accgttcggt ccgccggtaa tttcggtgtt attgagcagc aatatgcgca caatttcgcc
    90421 gaacccgagg gtaacgatcg ccagatagtc accgcgcaga cgcagcaccg gaaaaccgag
    90481 caggaagccc gccgccgccg ccattaaccc ggcaatcggc agacaggtcc agaagcccag
    90541 gccgtaatag tgattgagca gcgcaaaagt gtaagcgccg atggcgtaaa agccgccgta
    90601 tcccagcacc agcagaccag aaagaccaac aaccacgttc agcccgagac cgagaataat
    90661 gtagatcatg gtcagagtgg cgatatccac cgtcccacgc gacaccataa acggccacgc
    90721 cactgcaagc accagcagcg ccacgaggaa cagtttctgc ttcaccgtgg agccatcaat
    90781 cgcgggcaga ataaacttcg gcccggaaac gctttttagc cctttctgga aagcaggccg
    90841 cacaagctgg aagaaaaaga ccaccgccgt gccgataaac acccactgcc aacggatctc
    90901 tgaagccgtg tcgaccacca gtttggtgcc atccagctcc agttgcacgc ccataaagac
    90961 gcccgccagc acaaagaaca tagcggcaga gagcagcgcc attgcaatat gcatcggttt
    91021 catactttct ctacctccgg gcgacccaga ataccggtcg gcatcaccag cagcaccagg
    91081 atcagcaggg cgaatgagac cacatcttta tattccgtac tcagataggc agaagagagc
    91141 gcctccgcaa tccccaggat cagcccgcca atcatcgccc ccggaatgct gccaatccca
    91201 ccgagcaccg cagcggtaaa ggctttcatc ccggccataa agccgatgta ggggttaatt
    91261 acgccgtaga actgaccgag cagcacgccc gccactgccg ccatcgccgc gccaatcaca
    91321 aaggtcagcg caatcacccg gtcggtgttg atgccaagca ggctcgccat tttcagatct
    91381 tccgcgcagg cacggcaggc gcgccccata cgggaatagc gaatgaaaat cgtcagcgcc
    91441 agcatggcga ggaaagtaac aatccatatc accgcctgca tggtggtaat agacgcagag
    91501 aagttttcgc tatggcccac cacccactga ccgttaaaca ggctgggcag cgccacatcg
    91561 cgcgaacctt cggtcaggct gacgtagttt tgcaggaaga tagacatgcc gattgccgag
    91621 atgagtgcaa tcaggcgctt agagttgcgc accgggcgat aggctacccg ttcgatactc
    91681 cagccgtagg cgctggcaat gacgattgcg ccgacgaatc ccgccgccac cagcagccag
    91741 ccggtatcaa tgcccagcat catcagtgcg gcgatgatca taaaggagac gtagctgcca
    91801 atcatgtaaa cctcgccgtg ggcgaagttg atcatgccga taatgccgta aaccatggtg
    91861 tagccgatgg ctatcagcgc gtaggtactg cccagcgtga cgccgttaaa catctgctgc
    91921 aagaaataca aaaactgctc agacataagg taacctttct aaacccgccc gtttttcacg
    91981 gacggtggga tgatcacttg gctgccgtgg atgaaccgtc ggcgtgccac tggaagacac
    92041 caaaatcgaa tcccttaaga tcgccttttt catcccagtt cagcggccca attacggtgt
    92101 ttgcaccgtt agcttttaaa tctttcacca gcgccagcgg ctcatcgctg ccggtacgtt
    92161 caagggcagt tgccagagat tgcaccgccg cgtaggtgat ccagacatag ggcccggacg
    92221 gatctttctt gtcggctttc agcgcatcaa cgatgccctg gtttgtcgga tcctggtcat
    92281 agcgttttgg catggtgacc aacatgcctt cagcggcatc accggcaatg ttcgacagtg
    92341 acgcattacc gacaccttcc ggccccataa actgggtttt caggccaacg gaacgggcct
    92401 ggcgcagcat ctgccccatt tccgggtagt aaccgccgta gtaaacgaag tcgatgtttt
    92461 cttttttcag gcgggcgatc agcgcggaga aatctttctc tccagcggta atgccgtcga
    92521 agaagacgac gttggcgtta gccgctttca gcccgtcctg caccgaacgt gccagccctt
    92581 cgccatactg ttgtttgtcg tgaatgatgg cgatgcgctg gggcttcacc gtctcaagaa
    92641 tgtattttgc cgccgttggc ccctgggaag agtccagccc ggcagtacgc atgatgtatt
    92701 gataaccgcg ttgggtcagc tccggattgg tcgctcccgg cgagatcatc agaatacctt
    92761 cgtcttcata gatatctgat gcaggttggg tagaggagga acacaagtgc ccaataacgt
    92821 atttaatgcc gtcgttaacg attttgttgg cgaccgcaac ggcttgtttc gggtcgcagg
    92881 cgtcgtcata ttccacgcca accagtttat cgcccttaat tccccctttg gcattaatgt
    92941 ctttaattgc ctgacgcgcg ccgttaaatt ccatatcgcc ccactgggca atcgggccgg
    93001 acatcgcgcc gacaacggcg actttaatat cgtcagccat agcggtgtgt gaaattgcca
    93061 gtgcaatcat ccctgcgatg atagttttcg cattccgttt catagtcaaa aatccccatt
    93121 cgtgatgttg tgttgctttg ttcttatgtg ttaacaaatc agactgttct tttttatact
    93181 gcactgtttt tacctgtctg attaagggtt tagcgcagta ttttgtgatg atagcgatta
    93241 aaatccctat ttttcagtcg attaagaaca gataatattc tgaatttatt gatagataaa
    93301 cagaaaaaag cgccgttgtc agcataaaat aacggtacaa agggcagaat aattcactac
    93361 catttagggg attatgctgg atatttttca ttctctaatg ttttaacttt gtaattattg
    93421 ctgaaaaaaa attaatcacc tgccaaaaga aataaaaaag agaaagcctc cgattaaatt
    93481 atttcgctac actggttcca cttttgtgat ttacacgggt tccccatgaa gctgaccatc
    93541 attcgattag aaaactttag cgaccaggac cggattgacc tgcaaaagat ctggccggag
    93601 tattcccctt cctctttaca ggttgacgat aaccaccgca tctacgccgc gcgttttaac
    93661 gagcgcctgc tcgctgccgt gcgggtaacc ttaagcggca ccgagggggc actggattct
    93721 ctacgcgtgc gggaagtaac ccgacgtcgc ggtgtggggc aatatctgct ggaagaggtt
    93781 ttgcgtaaca atcctggcgt ttcatgttgg tggatggcgg atgctggcgt ggaagatcgc
    93841 ggcgtgatga cggcgtttat gcgagcactg ggatttacgg cacaacaggg tggctgggag
    93901 aagcgttaat cgtctggtgt aaaaagtagc tctatagatt tggtcgggaa aaataacgca
    93961 ttttcgtttt ctccgaccaa tgggataaag cccagcgact gataaaacgt atgggctttt
    94021 tcattcagcg cctcgacaaa aagaccgtga atacctaccg ctaaagaggc tgaccagaca
    94081 actttcatgg catgagcaac cagtgttgct ccccatccct gcccctgtaa tgaacgatca
    94141 attgccagac gcccaagagt tacgctggga atatttttgt agggaatttt tttctgtttc
    94201 gatttcgagg gcagtgcggc tcgttcaaaa caactgccgg ataatgtgta atatcccagc
    94261 acctgacgtt ctggagtgtt gcgacacagg atatacgctc gcagaatttt attgcgatgt
    94321 tgacgaacca gatgtgtcgt cagaaagaga tttaatgctt cctcgccgca gtcgaatcgc
    94381 tgtagatcat aatctgcatc atcggtcaga atctctatcg tcagatcatc cataaagtat
    94441 tacattccct gaagacgttt tgccgcacgt tttagctttt cacctggtga cggtggatta
    94501 ctcagcgcat ccatcacccg cgtccaggat tcctcagtga gaatcatccg ccgatgctgt
    94561 tcaatcactt ccgcagctcg ctgcgaagcg ctgttcaaca taaattggct gacggactga
    94621 ttagatatcg ctgccgcttc ctcaatcata cttttgtcat cgtcagttaa acgcagatca
    94681 atacgctgct ttttaacagc agacatgtga actctcctgc tccgtataat atgtacggca
    94741 tatcaccgta cataagcata tcctgaattg ttcaatcttt ctacaaccat ttcgataaat
    94801 ataaaaaaac ccgatggcag ttcagcgcat cgggtatttt catttatcta tctgttactt
    94861 cgcatcagtc gccgtgccgt tggcgtgcca gtcaaacaca ccgaactcaa agcctttcag
    94921 atcgcctttc tcatcccagc taagcggccc catcaccgta tcaaccgagt tcgctttcag
    94981 gtatttagcg atctccgccg gatcgtcaga ctgattcagc cccgcttgca aagattgcag
    95041 cgcggcgtag gtggtccaga cgaacgcgcc gcttgggtcc tgttttttcg ctttgatcgc
    95101 gtcaacaatc ggtttgttcg ccggaacctg atcgtagttc ttcggcttgg tcaccagcag
    95161 cccttccgct gattcgcccg caatgttaga cagcgagacg ttagccacgc cttctggccc
    95221 cataaactga gttttcagcc ctgccgcgcg tgcctgacgc aggatttgcc ccatttccgg
    95281 gtgataaccg ccgtagtaaa cgaaatcgat attctctttc ttcaaacgcg ccaccagggt
    95341 agagaaatct ttttccccgg cggtgatgcc atcaaagaac accacgttag cattgccttt
    95401 cttcaggccg tcctgcactg cacgcgccag accttcgccg tactgctgtt tgtcgtgaac
    95461 gatagcaatg cgttgcggtt tcactttctc aagaatatat ttcgccgccg tcggcccctg
    95521 gtcggagtcc aggccggtgg tgcgcaggat cagctgatag ccacgggcgg tcagctctgg
    95581 tgcggttgcc gctggggtga tcattaaaat gccttcgtct tcgtagatgt cagacgctgg
    95641 ctgcgttgat gaagagcaga ggtgaccaat cacgtattta atgccgtcgt taacgacttt
    95701 gttcgccacc gcaaccgcct gtttcgggtc acaggcatcg tcatatttta cgatttgcag
    95761 tttgttgcct ttgatacccc ctttagcatt gatatccgca accgcctgct ctgcgccagt
    95821 aaactcctgg tcaccgtact gcgccaccgg accggacatt gcgcctacga cggcgacttt
    95881 aatatcttct gccagagcca tattgctgaa tgccagcgcg atacatcctg ccagtaacgc
    95941 tttacccttt atgttcatcc tgagaatccc cactcttctg gttattacgt gtgttgtgat
    96001 gttgttgtgc agcactttat ttcgttttat acgtagctac ccgtgcttta gcagcatact
    96061 ctgctaaaac ataccccatt tttatgatat tggaatagct attttgacag tttattaaca
    96121 atctgcgtgg ggattggcgt tttgccggag ggggaattga ttattgtaga ggcccaaaaa
    96181 acaaaaaccc cggactctca tccagggttc tctgcttaat agcggaaatt acgcttcaat
    96241 agcagcgcgc aattttttca tcgcgttctt ttccagctga cgcacacgct cagcggaaac
    96301 accgtaacgg tcagccagtt cctgcaacgt ggacttgttg tcttcgtcca gccagcgcgc
    96361 gcggatgata tcctggctac gctcgtccag accttgcatc gcgtcggtca gacggtttgc
    96421 cgcctgctct tcccagttat catcttcgat gccgtcggca aagttagatg atttatcctg
    96481 cagatagagc accggagcca tcggctggct gtcggaatcg tcgtcggaag acaggtcaaa
    96541 ggtcatgtcc tgtgccgcca tacgtgattc catctcacgt acgtctttgc tggttacgcc
    96601 cagttcacgg gccaccatct cgacttcatc ctggttaaac cagcccagac gctgcttggt
    96661 tttacgcagg ttgaagaaca gtttgcgttg cgctttggtg gtcgcaactt tgacgatacg
    96721 ccagttacgc agaacgtatt cgtggatctc tgctttgatc cagtgaacgg cgaaggagac
    96781 caggcgcaca cccacttccg ggttaaaacg gcgcactgct ttcatcaggc cgatgttacc
    96841 ttcctgaatc aaatccgcct gtggcaggcc atagcccgca taattacgag caatatgaac
    96901 aacaaaccgc aggtgagaca ggatcagcgt tttagctgct tccagatcgc catggtaatg
    96961 cagcttttca gccagcgccc gctcctcgtc agccgacaac atcggccacg cgttagctgc
    97021 ccggatgtag gaatccaggt tgccaactgg ggctaaagct aaactttgca ttttgtcagt
    97081 cattcaaatc ctctcaatcg atatcttctg gcgcttcagt ggtagcaaca actgtgccag
    97141 agcttaagag caacgaggtt atcattcact gttttatcag accgtgattt tatccacaag
    97201 ttcaatgcaa gtttgtgaat aaattacgca caaaatgtga catagagata aaataccggg
    97261 aagagacaac ggggtctctt tccctgctac ggaatccatt gcagggaaag agtataacac
    97321 gcttttatta ttcaggcgta aagtggcgta aatgttgtac cgtggcaagc cacgctgcca
    97381 cccagccaat catcgagcat accagcagca atagcaggca ttcatcgaac gataagccat
    97441 tgatatcaaa cttcgttccg aaaacctgtg ccacttccgc aaccgccgat gacaaacgca
    97501 gcaccagaat ttctgacaaa attagtgaca acaatgcgcc agaaaatccc agcaatgcgc
    97561 caccgtacag gaacggacgc aggatgaaac catccgtcgc accaatcagt ttctgcacgt
    97621 taatagaatc acggcgagca aagatactca ggcgcacact gttaccgatg acgaggaata
    97681 ctgccgccac catcaatacg ccgatcattg ccgaaacgcg cccgaccagc ccggtcaacg
    97741 ccgccaggcg ggcaaaccag ctgtcatcca tccgcacttc gtcaatgccg ttaatctgcg
    97801 tgatacgatc acgcagcgta ttcagtgact ccgtcccctg aaaatcgagt ttcgggatca
    97861 ccaccgccac tgccggaagc gggttttctt ccagcatatc cagcgcacca ccaaaaccag
    97921 accagttacg gaactcgccc agtgcgtctt cacgagaaag ataattcact ttctccacgc
    97981 cttgctcggc ctgcaactgt gccaccacgc ccgcagcggc gtcatcatcc agcgtttttt
    98041 gcagataaac agtgatttgc ggtgacggat aatattgcgt cgccgcctgg ttaacgtttt
    98101 tgtacaccat ataacagacg ctgggcagcg tcagggagat ggcgataacc atcaccgtta
    98161 aaaacgtggc gaacggtttg cttttcagat cctgcaatgc gccgtggaag gcatagcgta
    98221 cctgttcgtt gaaaacgttg gttttgcgat ttaccggttt tggcgaggat ttcgcgcgtt
    98281 tcggcgcgtt acgaccgccg tcgccagagc cgccgaccga tttacggaag cgatccagac
    98341 gcccgccaaa ctgccgaatg tgattgattg catcgcgctt attcatggcc cacgcctcca
    98401 tgcaagtgac catcgctcag ggtgagcatg cgataggaac gtcgcgaaat caggttgatg
    98461 tcgtgcgttg ccatcaatac ggttactcca acgcggttaa actcttcaaa cagacgtaaa
    98521 atgccttccg acagcgcgtc gtccaggtta ccagtcggtt cgtccgccag cagtaccgcg
    98581 ggcttgttca ccaccgcgcg ggcaatgcca acacgctgtt gttcaccgcc ggaaagctga
    98641 atagggaagt tcttcgcttt gtccagtagc ccgactttat ccagcgccgc cgacacccgg
    98701 cgacgaatgt catcaccgct ggcaccggcg ataatcagcg ggatcgccac gttatcatag
    98761 acagtacggt ccatcagtag atggtgatcc tggaaaatca tgccaatctg gcggcgcaga
    98821 aacggaactt cacggttttt cagacgcgtg atgtcatggc cgctaaacca gattttcccg
    98881 gcgctgggcc gctcaatccc acagatcagc ttcaggaggg tacttttccc tgcgccggaa
    98941 tgaccggtca gaaacgccat ctcacccggc tgcatatgga acgtaacgcc ctgcagcgcc
    99001 tgtctcccac cgagataagc cttgctgaca tgttcaaagc gaatcattgt taatcctctc
    99061 gggcaaaaag tgcctctata aagtcgtccg ccttaaacgg acgcaaatcc tcaatacgtt
    99121 cgccgacacc aatgtagcgg atagggatac caaactggtc agccaccgag aaaattaccc
    99181 cgcctttcgc cgtgccgtcc agtttcgtta gcgtgatgcc ggttaagcca acggcttcgt
    99241 ggaacagttt ggcctggctt accgcattct gcccggtgct ggcatcgatg gtcagcataa
    99301 cttcatgcgg agcttcaacg tcgagtttct tcatcacgcg gacgattttc ttcaactctt
    99361 ccatcaggtg cgatttgttt tgcaggcgtc cggcggtatc ggcaatcagg acgtcgatgt
    99421 tacgcgcttt agcggcctga atggcatcga agataacaga agcggaatcc gcaccggtat
    99481 gctgggcaat caccggaata ttgttgcgca gaccccagac ctgaagctgt tcaaccgctg
    99541 ccgcacggaa agtatcgcct gccgccagca tcaccgattt accctgctgc tcaaactgac
    99601 gcgccagctt accaatcgtc gtggttttac ccacaccgtt gacgcccacc atcaggatca
    99661 cgaacggcgt tttaccttca acattcagcg gctcatcgac tttcgccaga atctcgccca
    99721 tctcttcttt cagcaggcca tagagcgcct cggcatcacg aagctgcttg cgggatgcgc
    99781 cttctgtcag attggtgata attttacgag tggtttccac gcccacatcg gcgatcaaca
    99841 gctgttcttc cagctcttca aacagatcat cgtcgatttt tttaccgcgg aacaggctga
    99901 taaatccgga accgagattt tctttggttt ttaacaggct gcgtttcagg cgcgcgaaga
    99961 aaccttcttt ggtcggtttt tcctgctcct gagcgatttc ttccaccggc tgatcttctt
   100021 ctgccggaga aaccaccatt accgcctctt ctgccacttc ggcagccagc gcctgtgcct
   100081 ccggctcttc gtcggtgatt tcttctttag ccgcttcttc ttccgccgct tcgacaatct
   100141 ctacggtttc cgcttcggcc tgccactctt ctggcgaaac cgcttcggcg ttgacgtctt
   100201 ccggcaacgg cagttcttca cgttcgatag ccactggctc cggcgtttct tctacgaccg
   100261 gttccggctg tgcaacgact tccgcttcag gctgcgcttt ttcactttca gcaacctgtt
   100321 cagtaacttc cacaacgtcg gcagcaaaag tttccgcctc ggcttcagta tgcgcctgcg
   100381 gctgctcttc aacggcttgt tcagaagcct tcacaggctc ttgctcctga acgatttctt
   100441 ctacaactgg ttgttcattc tgaacttctg tctctttttc cggggtctgc tctttttgac
   100501 caaagcccag ccaggaaaaa aagccacgtt ttttttcttt cgccatttgc gactacactc
   100561 ctcgctgttg cttcatggca cagcgttaac gctatgtaca tagcagctaa aaaaatgatg
   100621 aaatagtcta tcacttaact taattcacat caccgcctgc aagtatgtgt tatctggcgg
   100681 attgagcaat ttatcatgaa aaaaccgaat cattccggca gcggccaaat ccgcattatt
   100741 ggcgggcagt ggcgaggccg taaactcccg gttcctgata gtccaggtct gcgccccacc
   100801 accgaccgcg tacgcgaaac gttgtttaac tggctggctc cggtcattgt tgacgcccaa
   100861 tgtctggatt gcttcgccgg gagcggcgcg ctggggctgg aagcgttatc gcgctacgct
   100921 gcgggggcca cgttgattga gatggatcgt gcggtttctc agcagttaat taagaatctg
   100981 gcgacactaa aagcaggcaa tgcacgcgtg gtgaacagca acgcgatgtc attcctggcg
   101041 caaaaaggca caccgcataa tatcgtgttt gtcgatccac cgttccgccg tggcttgtta
   101101 gaagagacga taaatttact ggaagataac ggctggctgg ctgacgaagc cctgatttat
   101161 gtcgaaagcg aagtcgaaaa gggtctgccc actgttccgg caaactggtc attacatcgg
   101221 gaaaaagtgg cgggtcaggt ggcttatcgg ctgtatcaac gcgaagcaca aggagaaagt
   101281 gatgctgatt aatattggtc gtttgttaat gctctgtgtt tggggatttt taatcctcaa
   101341 cctggtgcat cccttcccac gcccgctgaa tattttcgtt aacgtggcgc tgatttttac
   101401 cgtgctgatg catggtatgc agctggcgct attgaaatcc actttaccga aagatggccc
   101461 gcagatgacc accgccgaaa aggtgcggat tttccttttc ggcgtgtttg aactgctggt
   101521 ctggcagaag aaatttaaag ttaaaaaata accttattgt tcgcctacaa agctgacaaa
   101581 gcgcgttcct ttatagctca gcgtaccttt atcgcccact gtcagggcgt ggtactgctg
   101641 ggcgtcgagg cgaaacgtct gctccattcc tccgctttgc ggtttgaagc ttgcctcata
   101701 gcgcatactg gtgcctgccg gagtcacttc ctgctggcgc gaacggcgat cattaatcgg
   101761 tttttcccgc ttgttgctca ccaccaccag cttttgctgg agcggtgcca tatcattatc
   101821 agctttttcc cgtcgttgtt gcataaaacg aaacgatgcg gcgacgatga ttaagccaat
   101881 gataacaata aagaaaagag gtggtttact catctttatc cctcatcgga aaatgcggaa
   101941 ataagcatac cctgccagtt atggtgttgt catccgtcca ccctcgccac taaactggaa
   102001 gcaagaccgt aggcattccg cttacgaaaa aataacgaat tcaaggaact aagatgcttt
   102061 ggtcgtttat cgctgtctgt ctttccgcat ggctatctgt ggatgcatcg tatcgtgggc
   102121 caacctggca acgctgggtg tttaaaccgt taacccttct tctcctgctg ttgctggcct
   102181 ggcaagcgcc gatgttcgac gccattagct atctggtgct ggcggggctg tgtgcctcac
   102241 tgctgggcga tgcgctaacc ctgttgccac gtcaacgtct gatgtacgcc atcggcgcgt
   102301 ttttcctctc gcacctgctg tacaccatct atttcgccag tcagatgacg ctctctttct
   102361 tctggcctct gccgttggtg ctgctggttc tgggcgcgct gttactggcg attatctgga
   102421 cgcgtctgga agagtatcgt tggcctatct gcacgtttat cggcatgacg ctggtgatgg
   102481 tgtggctggc aggtgaactg tggttcttcc gtccgaccgc tccggcgctc tctgcgtttg
   102541 ttggcgcttc attgctgttt atcagtaact ttgtctggct gggtagccac tatcgccgcc
   102601 gcttccgggc agataacgcc attgccgcgg cctgctactt tgctggtcac ttcctcatcg
   102661 tccgctcgct gtatctctga taaaacttga ctctggagtc gactccagag tgtatccttc
   102721 ggttaatgag aaaaaactta accggaggat gccatgtcga ctcctgacaa tcacggcaag
   102781 aaagcccctc aatttgctgc gttcaaaccg ctaaccacgg tacagaacgc caacgactgt
   102841 tgctgcgacg gcgcatgttc cagcacgcca actctctctg aaaacgtctc cggcacccgc
   102901 tatagctgga aagtcagcgg tatggactgc gccgcctgtg cgcgcaaggt agaaaatgcc
   102961 gtgcgccagc ttgcaggcgt gaatcaggta caggtgttgt tcgccaccga aaaactggtg
   103021 gtggatgccg acaatgacat ccgggcacaa gttgaatctg cggtgcaaaa agcgggctat
   103081 tccctgcgcg atgaacaggc taccgacgaa ccacaagaat cacgcctgaa agagaatctg
   103141 ccgctgatta cgcttatcgt gatgatggca atcagttggg ggctggagca gttcaatcat
   103201 ccgtttggtc aactggcgtt tatcgcgacc acgctggttg ggctgtaccc aattgctcgt
   103261 caggcattac ggctgatcaa atctggcagc tacttcgcca ttgaaacctt aatgagcgta
   103321 gccgctattg gtgcgctgtt tattggcgca acggctgaag ctgcgatggt gttgctgctg
   103381 tttttgattg gtgaacgact ggaaggctgg gccgccagcc gcgcgcgtca gggcgttagc
   103441 gcgttaatgg cgctgaagcc agaaaccgcc acgcgtctgc gtaacggtga gcgtgaagag
   103501 gtggcaataa acagcctgcg gcctggcgat gtgattgaag ttgcggcagg tgggcgtttg
   103561 cctgccgacg gtaaactgct ctcaccgttt gccagttttg atgaaagtgc cctgaccggc
   103621 gaatccattc cggtggagcg cgcgacgggc gataaagttc ctgcaggcgc caccagcgta
   103681 gaccgtctgg taacgctgga agtgctgtca gaaccgggtg ccagcgccat tgaccggatt
   103741 ctgaaactga ttgaagaagc cgaagagcgc cgcgcaccta ttgagcggtt tatcgaccgt
   103801 ttcagccgta tttacacgcc agcgattatg gcggtcgccc tgctggtaac gctggtgccg
   103861 ccgctgctgt ttgccgccag ctggcaggag tggatttata aagggctgac gctgctgctg
   103921 attggttgcc cgtgtgcttt agttatctcc acgcccgcgg cgattacctc cgggctggcg
   103981 gcagcagcgc gtcgtggggc gttgattaaa ggcggcgcgg cgctggagca gctgggtcgt
   104041 gttacccagg tggcgtttga taaaaccggt acgctgaccg tcggtaaacc gcgcgttacc
   104101 gcgattcatc cggcaacggg tattagtgaa tctgaactgc tgacactggc ggcggcggtc
   104161 gagcaaggcg cgacgcatcc actggcacag gccattgtgc gcgaagcaca ggttgctgaa
   104221 ctcgccattc ccaccgccca atcacagcgg gcgctggtcg ggtctggcat tgaagcgcag
   104281 gttaacggtg agcgcgtgtt gatatgcgct gccggaaaac atcctgctga tgcatttgct
   104341 ggtttgatta atgaactgga aagcgccggg caaacggtag tgctggtagt acgtaatgat
   104401 gacgtgctgg gtgtcattgc gttgcaggat accctgcgcg ccgatgctgc aactgctatc
   104461 agtgaactga acgcgctggg cgtcaaaggg gtgatcctca ccggcgataa tccacgcgca
   104521 gcagcggcaa tcgccgggga actggggctg gaatttaaag cgggcctctt gccggaagat
   104581 aaagtcaaag cggtgaccga gctgaatcaa catgcgccgc tggcgatggt cggtgatggt
   104641 attaacgacg cgccagcgat gaaagctgcc gccatcggga ttgcaatggg tagcggcaca
   104701 gacgtggcgc tggaaaccgc cgatgcagca ttaacccata accacctgcg cggtctggtg
   104761 caaatgattg aactggcacg cgccactcac gccaatatcc gccagaacat cactattgcg
   104821 ctggggctga aaggcgtttt cctcgtcacg acgctgttag ggatgaccgg actgtggctg
   104881 gcggtgctgg cagatacggg tgcgacggtg ctggtaacgg cgaatgcgtt aaggctgttg
   104941 cgcaggaaat aaggcaaacc gatcgcaaca ttgagcgcga tcggtcccct cgctcctctg
   105001 gggagagggt tagggtgagg ggaaaaggcg gcatcgaagc caatcttccc ccatcaaccg
   105061 cctttacgaa tcaaataacg ataaggcagt ccatcggtct ctttcgcaac cagttcgtgt
   105121 tccataaagg tacaaaaccc aggaatatcg cgtgtcgtcg ccggatcgtc agcaataatc
   105181 agcaacgttt cacctggctg catattgcgc acagttttgc gcaccatcat tactggttcc
   105241 gggcagcgca ggccaagtgc gtcgagtgtg tggtcagggc tggaaaagag atcggtcatt
   105301 ttcttctcat cacataaaaa aacggcgcta gtttacgccc tgtgagtccg taagcaaacc
   105361 aggttaacga ttgcgtgaaa attaaccatt gcattgttaa cgtaaagcag tatcatgcgg
   105421 cggctcgaaa aaagggtcag cacgttatta tgttaaggta acagacgtgt cgtacgtatt
   105481 gggttccctc accccaatgg ttaatcaaaa aggtacaata tgaacgtttt ctcgcaaacg
   105541 caacgctata aggcgttgtt ctggttatcg ttatttcatc ttctggtgat cacctccagt
   105601 aactatctgg ttcagcttcc ggtctccatt tttggttttc ataccacctg gggcgcgttt
   105661 agctttccgt ttatctttct cgctactgac ctgaccgtgc gtatttttgg cgcgccgctg
   105721 gctcgtcgca tcatctttgc ggtaatgatc cccgcattgc tgatctccta cgtcatctca
   105781 tcgctgtttt atatggggtc atggcaagga ttcggcgcgc tcgcccactt caacctgttt
   105841 gtcgcccgta tcgccaccgc cagtttcatg gcctacgcgc tggggcagat cctcgacgtg
   105901 cacgttttta accgcctgcg tcagagtcgt cactggtggc tggcaccgac agcgtccaca
   105961 ctgttcggta acgtcagcga cacgctggcc ttcttcttca ttgccttctg gcgtagcccg
   106021 gatgccttta tggctgaaca ctggatggaa atcgcgctgg tcgattactg tttcaaagtg
   106081 ttaatcagta tcgttttctt cctgccaatg tatggcgtat tactcaatat gctgttgaaa
   106141 agactggcag ataaatccga aatcaacgct ttgcaggcga gttaaaggtt cgttatcaga
   106201 gttgtgataa gatggatgaa tgagccgtta tggccgttta tcgaaaggaa gaagtcaatg
   106261 cgcaatctgg ttaaatatgt cggaattggc ctgctggtta tgggacttgc ggcctgtgat
   106321 gataaagaca ctaacgctac ggcgcagggt tcggtcgcag aaagtaacgc gaccgggaat
   106381 cccgtcaacc tgcttgatgg caagttaagt ttctcgctgc cagcggatat gaccgaccag
   106441 agcggtaagc tgggaacgca ggccaataac atgcatgtct ggtccgacgc caccgggcag
   106501 aaagcagtca tcgtcatcat gggcgatgat ccgaaagaag atctggcggt gctggcgaag
   106561 cgtctggaag atcagcaacg tagccgcgat ccgcagctgc aagtggtaac caataaagcc
   106621 attgagctga aaggtcacaa aatgcagcag ttagacagca ttatctccgc gaaaggccag
   106681 acggcgtact cttccgttat tctgggtaac gtgggtaatc aactgctaac catgcaaatt
   106741 acgctgcccg ctgacaatca gcaaaaagca cagaccaccg cagaaaacat cattaatacg
   106801 ctggttattc agtaagtttt aagatgatga ggcggcctca ggtatttcca ccgggggccg
   106861 tttttttaat cgccacgtca gtaataacgc gattgcgacc agtcccgccg ccgccagata
   106921 aatcaccgga acgcccgccc aactcatcac cagcccagcc agtggtcccg tcacgccaag
   106981 cgataaatcc ataaatacgg tgtaagttgc cagcgccgcc ccctgatttt gctgcggaac
   107041 cgcttttacc gccactacgc ccaatgcagg gaacaccagc gaaaaccccg cccccgccag
   107101 taagacgccg attttcgcca tccacggcat agtcgccacg ccaaccagta gcaggccgat
   107161 tatctcaacg ctaaagcaaa tcatcgccac gtttaagccg ccgatacggt taatgccgtt
   107221 agggaataac aaacgcgtac cgacaaacgc acagctaaac agcgtcagcg cgaaagccgc
   107281 accgtcccaa cctttagcgt cataaaacag cgtgataaag gtggcgataa cgccaaatcc
   107341 ggcggaagcc agtgccagcg ccataccgta cagccagacg cgcccaagca ccgcacgaaa
   107401 cggcagcggt ttgcctttac tggcttttac cgtcggacgc gggatcgcca acaaaatggc
   107461 caccagtgcc acgcccataa tgattaacgc taacgcctgc aatccgcccc agtgataaaa
   107521 cacgacacct aacggcgcac ccatcgccat cgccccgtaa gtgacaatac cgttccacga
   107581 aatcacccgc ccgatatgta gcgaaccaac cacgccaaca ccccacaggg tcgatcccgt
   107641 tccggcaaaa ctttgtccaa tcccaaggat tacgcgcccc aggcacagta ataacaggct
   107701 gatgacgggc agactggcgg ttaatcctgc cgtcagatat cccagaccgc tcataaagca
   107761 gccgcataaa ccgaagacga caatcttttt gggtcccagc aaatcggcgt aacgtccggc
   107821 atgaggacgg ctcagcaagg tggcgaaata ttgcaggctg ataaccaacc ctgcccagaa
   107881 ggcactaaag cccatcacat catggacata gcccggtaat acagcgagcg gcaacccgat
   107941 ggtgaggtag ctggcgaagt taaacatgac aatggagaca atgcgcaaat tcaggcgcaa
   108001 tccgtttagc gcgggttcgg ctacgggttc gggcatgagg atcaccacat ttttacaaca
   108061 gtgtttcatt tttaccacgt gctgacgtga aaatcagcag taagaatcag aatattgctg
   108121 gcgtgactcc cgctacactt aatacaaaaa gtcacaagga agccccaatg gaaacccctc
   108181 aacccgataa aacgggcatg cgcattctgc tcaagttggc ctccctggta gtgatcctcg
   108241 cgggcattca cgcagcggca gatatcattg tgcagctact actggcgctg ttttttgcca
   108301 tcgttctcaa cccgctcgtc acctggttta ttcgtcgggg agtacaacgc cccgttgcca
   108361 ttacgattgt ggttgtggtg atgctgatcg cactcaccgc gctggtcggc gtactggcgg
   108421 catcgtttaa cgaatttatc tctatgctgc cgaagtttaa taaggagctg acgcgcaaac
   108481 tttttaaatt gcaggagatg ttgccttttc ttaatttgca tatgtcgccg gagcgaatac
   108541 tacagcggat ggactcggaa aaagtggtta ccttcaccac ggcgctaatg accgggcttt
   108601 ccggggcaat ggcgagcgtg cttttgctgg tgatgaccgt agtttttatg ctgtttgaag
   108661 tgcgccacgt cccttacaaa atgcgtttcg cgttgaataa tccacagatt cacatcgccg
   108721 gactacaccg cgcacttaaa ggcgtttcgc attatctggc gttaaagacg ctactcagtt
   108781 tatggacagg cgtaatcatc tggctggggt tggagctgat gggggtgcag tttgcgctga
   108841 tgtgggcagt actggcgttt ttgctcaact acgtgcccaa tatcggtgcg gtaatttccg
   108901 ccgtaccgcc aatgattcag gtgctgctgt ttaatggtat ttacgaatgt attctggtcg
   108961 gcgcattgtt tttagtggtc catatggtca tcggcaatat tttagaacca cggatgatgg
   109021 gccatcgcct ggggatgtcc accatggtgg tatttctttc attgttaatt tggggatggc
   109081 tgctcggccc ggtagggatg ctactttcgg tgccattaac cagcgtgtgt aaaatctgga
   109141 tggaaaccac caaaggcggc agcaaactgg cgattttact ggggccaggc agaccgaaaa
   109201 gtcggttacc gggatgaggc gacaagtgtt acgatacgca ctttcatttt ccattaaacg
   109261 ttggccctga tatgtatcgg atagttctgg ggaaagtttc gaccttaagc gcagctcctc
   109321 tgccaccggg tttacgcgag caagcaccgc aaggtccacg acgcgaacgc tggctggcgg
   109381 ggcgtgcatt gctttcgcac acgctttccc cgctaccgga gatcatctat ggcgaacaag
   109441 gcaaacctgc attcgcgccg gaaacgccgc tatggttcaa cttaagccat agcggtgacg
   109501 atatcgccct gctgttgagc gatgaaggcg aagtcggctg cgatatcgaa gtgattcgcc
   109561 cgcgcgccaa ctggcgctgg ctggcgaacg ccgtattcag ccttggggaa catgccgaga
   109621 tggacgccgt acatcctgat cagcaactgg aaatgttctg gcgcatctgg acgcgcaaag
   109681 aagccatcgt taaacagcgt ggcggcagcg cctggcaaat cgtcagcgta gacagcacct
   109741 ataactcctc gctgtcagtc agccattgtc agctagaaaa tttaagcctg gcgatctgca
   109801 cccctacccc ctttacgctc accgccgaca gtgtgcaatg gatcgattca gttaactgat
   109861 ccgcccaccc gactgcccat ctattgatcc agaacaggta atcagtatga cgaatactta
   109921 aaatcgtcat acttatttcc gccatctatt ttaatccatt ggggttacca tgttctctac
   109981 actccgccgc actctatttg cgctgctggc ttgtgcgtct tttatcgtcc atgccgctgc
   110041 accagatgaa atcaccaccg cctggccggt gaatgtcggg ccactaaacc cgcaccttta
   110101 cacgcctaac caaatgttcg cccagagcat ggtttatgaa ccgctggtga aatatcaggc
   110161 agacggttcg gtgatcccgt ggctggcaaa aagctggacc cattcggaag atggcaaaac
   110221 ctggaccttc accttgcgtg atgacgtgaa attctccaac ggcgaaccgt ttgatgcaga
   110281 ggcggcggca gaaaacttcc gtgcagtgct cgataaccgt caacgtcacg cctggctgga
   110341 gctggcaaac cagattgttg atgtcaaagc actcagcaaa aacgagctgc aaatcaccct
   110401 gaaaagcgcc tactatcctt tcctgcaaga actggccctg ccgcgtcctt tccgctttat
   110461 tgccccgtca caatttaaaa atcacgaaac catgaacggg ataaaagcgc cgattggcac
   110521 cggaccgtgg gttttgcagg aatcgaaact gaatcagtac gatgtcttcg tccgtaacga
   110581 aaactactgg ggcgaaaagc cggcgattaa aaagatcacc tttaacgtga tcccggaccc
   110641 gaccacccgc gcggtggcgt ttgaaaccgg cgatatcgac ctgctgtacg gaaacgaagg
   110701 gttattaccg ctcgatacct tcgcccgctt tagccagaac ccggcttacc acactcaact
   110761 gtcacagccg atcgaaaccg tgatgctggc gctcaatacc gccaaagccc ccaccaacga
   110821 gctggcagta cgtgaagctc ttaattacgc ggtgaacaaa aaatcgctga ttgataacgc
   110881 gttgtatggt acccagcagg tcgccgacac cctgtttgcc ccttctgtgc cctacgccaa
   110941 cctcggcctg aaaccgcgcc agtacgatcc gcagaaagcg aaagcgttgc tggaaaaagc
   111001 cggttggacg ctgcctgctg gcaaagacat ccgcgagaaa aatggtcagc cgctgcgcat
   111061 tgaactgtcg ttcatcggca ccgatgcgtt aagcaaatcg atggcggaaa tcattcaggc
   111121 tgatatgcgc cagattggcg cagatgtctc gctgattggc gaagaagaga gcagtatcta
   111181 tgctcgtcag cgcgacggtc gttttggcat gattttccac cgcacctggg gcgcgccata
   111241 cgatccacac gccttcctga gttcaatgcg cgtaccgtcg cacgctgact tccaggcaca
   111301 gcaaggttta gccgacaagc cgctgattga taaagagatc ggcgaagtgc tggcgaccca
   111361 tgacgaaacg caacgtcagg cgctgtatcg cgacattctg acccgtctgc atgacgaggc
   111421 ggtttatctg cctatcagtt acatctcaat gatggtggtt tcaaaaccgg aactgggtaa
   111481 catcccctac gcgccgatcg ccaccgaaat cccgttcgaa cagattaaac cggtgaaacc
   111541 ttaatgttgc gttatgtatt acgccgcttt ctgctgctga tcccgatggt gctcgccgcc
   111601 tcggtgatca tttttctgat gctgcgcctc ggcaccggtg acccggcgct cgattatctc
   111661 cgtctgtcta acctgccgcc gacgccggag atgctggcct ctacccgcac tatgctgggg
   111721 ctggatcagc cgctgtacgt ccagtatggc acctggttgt ggaaggcgct gcatcttgac
   111781 tttggtatct cattcgccag ccaacgcccg gtgctggacg atatgctgaa tttcctgccc
   111841 gccacgctgg aacttgcagg tgcggcgctg gtattaattc tgctcacctc cgtaccgctc
   111901 ggtatctggg cggcgcgcca tcgcgatcgt ctgccggatt tcgccgtacg ttttatcgcg
   111961 tttctcggcg tgtcgatgcc taacttctgg ctggcgtttt tactggtgat ggcgttttcg
   112021 gtgtatctgc aatggctacc cgcgatgggt tacggcggct ggcagcacat cattttgcct
   112081 gcggtatcca tcgcctttat gtcgctggcg attaacgcgc gtttgctgcg cgccagtatg
   112141 ctggacgtcg ccggtcagcg tcatgtgacc tgggcgcggc tgcgcggcct gaatgacaaa
   112201 cagaccgaac gccgccatat tctgcgcaat gcctcgctgc cgatgatcac tgccgtgggg
   112261 atgcacattg gcgaactgat tggcgggacg atgattatcg aaaacatctt tgcctggccg
   112321 ggcgtcgggc gctatgcggt gtcggcgatt tttaaccgtg actatccggt gatccagtgc
   112381 tttacgctga tgatggtggt ggtttttgtg gtctgtaatt tgattgtcga tttgctcaac
   112441 gccgcgctgg acccgcgtat tcgtcgtcat gaaggagcgc acgcgtgaac tttttcctct
   112501 cctcccgctg gtcggttcgc ctggcgctga tcattatcgc cctgctggcg ctgattgcgc
   112561 tcaccagcca gtggtggctg ccgtatgacc ctcaggctat tgatttgccg tcgcgcctgc
   112621 tctcgccgga tgcgcagcac tggctgggca ccgatcattt agggcgcgat attttctcgc
   112681 ggctgatggc agcgacccgc gtgtcgctcg gctcggtaat ggcctgcctg ctgttggtgc
   112741 tgacattagg gctgattatt ggcggcagcg ccgggctgat tggcgggcgc gttgatcagg
   112801 ccaccatgcg cgtcgcggat atgtttatga ccttcccaac ctcgattctg tcgttcttta
   112861 tggtcggcgt gcttggcacc gggttgacca acgtaattat cgctatcgcc ctttcgcact
   112921 gggcatggta cgcgcgcatg gtgcgcagtc tggtgatttc actgcgccag cgcgagtttg
   112981 tgctggcatc acggctttct ggcgcgggcc atgtgcgggt gtttgttgat catctggcag
   113041 gtgcggtgat cccctcgctg ctggtgctgg cgacgctgga tatcggtcat atgatgctac
   113101 acgtcgcggg gatgtccttc ctcggtctcg gcgtgaccgc gccgaccgcc gaatggggcg
   113161 tgatgattaa cgacgcgcgt cagtatatct ggacccagcc gctgcaaatg ttctggccgg
   113221 ggctggcgct gtttatcagc gtgatggcct ttaacctggt gggtgacgca ctgcgcgatc
   113281 atctggaccc tcatctggtg acggagcacg cacactaatg ccacaacaga ttgaactccg
   113341 taatatcgcg ctacaggccg cgcagccgct ggtgcacggc gtatcgttaa ccctgcaacg
   113401 tgggcgtgtg ctggcgttag tcggcggtag tggcagcggg aagtcgctga cctgcgccgc
   113461 gacgctgggc attctgcccg caggcgttcg ccagacggcg ggggaaattt tggccgatgg
   113521 aaaaccggtt tctccctgcg ccctgcgcgg catcaaaatt gccaccatca tgcagaaccc
   113581 gcgcagtgcc tttaatccgc tgcacaccat gcacacccac gcgcgtgaaa cctgcctggc
   113641 gctggggaaa cccgccgatg acgctacgct taccgctgcc atagaagcgg tggggctgga
   113701 aaacgccgcg cgcgtgctga agctctaccc gttcgagatg agcggcggca tgttgcagcg
   113761 catgatgatt gcgatggcgg tgctgtgcga atcaccgttt atcatcgccg atgaaccaac
   113821 caccgatctc gacgtggtag cacaagcacg catcctcgat ctactggaaa gcattatgca
   113881 aaaacaagca ccgggaatgc tgctggttac ccatgatatg ggcgtagtag cgcgtctggc
   113941 agacgatgtg gcggtaatgt ctcacggtaa aattgtcgaa cagggcaatg tagaaacgct
   114001 gtttaacgcc cccaaacatg cggtaacgcg cagcctggtt tccgctcatc tcgccctcta
   114061 cggtatggag ctggcatcat gactttactt aacgtctccg acctttccca tcactacgcg
   114121 cacggtggat ttagcggcaa acatcaacac caggcggtgc tgaataacgt ttccctggcc
   114181 ctgaaaagcg gtgaaaccgt cgccctgctg ggacgcagcg gctgcggaaa aagtaccctc
   114241 gcgcggctgc tggtagggtt agagtcgccc tcacagggaa atattagctg gcgcggcgaa
   114301 ccgttggcga agctcaatcg cgcgcaacgt aaagcgttcc gccgtgatat tcaaatggtg
   114361 tttcaggact ccatcagcgc cgtgaatccg cgcaaaaccg tgcgtgagat cctgcgtgaa
   114421 ccgatgcgcc acctgctatc gctgaaaaaa gccgaacaac tagcgcgcgc cagtgaaatg
   114481 ctgaaggcgg tcgatctcga cgacagcgtg ctcgacaaac gcccaccgca gttaagcggc
   114541 ggccagctcc agcgtgtctg cctggcacgc gcgctggcgg tcgaaccgaa actgctgatt
   114601 ctggatgaag ccgtttcgaa ccttgatctc gtgttacagg cgggcgtcat tagcctgctg
   114661 aaaaagctgc aacaacagtt tggcaccgcc tgcctgttca tcacccacga cttacgcctg
   114721 gtggaacgct tctgtcagcg ggtgatggtg atggacaacg gacaaatcgt cgaaacccag
   114781 gtggtgggag ataaattaac cttttcctct gacgccggac gtgtgctaca aaacgcggta
   114841 ttacccgcat tccccgtgcg ccgtcgcacc acagaaaagg tttaacgcaa atgcaacgag
   114901 tcaccatcac gcttgatgac gatttactgg agacgctgga cagtctgagc cagcgtcgtg
   114961 gttataacaa ccgttccgag gcgatccgcg atattctgcg tagcgctctg gcgcaggaag
   115021 ccactcaaca acacggcaca caaggtttcg cggtgctgtc gtatgtgtat gaacacgaaa
   115081 aacgtgactt agccagccgt attgtctcca cccagcatca tcaccacgat ctctcggttg
   115141 ctactctgca tgtgcacatt aatcacgacg actgtctgga aatcgccgtg ttgaaaggtg
   115201 acatgggtga cgtgcagcat tttgccgatg acgtcatcgc ccagcgcggc gtgcggcacg
   115261 ggcatttgca gtgcctgccg aaggaagatt aacacctccc aacgcgccgt tatttcctga
   115321 cggcgcagta gacttctgcc aaatatcgac tcacgtcaca tttttttgat cacgatttgg
   115381 tcccctaaaa tgtgagacgc ttcgatcctc tttttcgcct gttcacctac tatacgcttg
   115441 aagttgtcca tgattgtcca tagacaactg taaatgttca ggtgcgcgcc atgaaatcat
   115501 tgagtaagtc gtcacaaata ccgctctatc aacaagtggt ggagtggata agagaaagta
   115561 tttataccgg ggatctggtg gaagacgatc gcattccttc ggaataccag attatggata
   115621 tgctggaagt gagtcgggga accgttaaaa aagcggtcgc ccaactggta aaagaaggcg
   115681 tgttgattca ggttcagggg aagggaacat ttgtcaaaaa agagaacgtg gcatatccgt
   115741 taggtgaagg attattgtca ttcgcggaat cgctggaaag ccagaaaata cattttacca
   115801 ctgaagttat tacgtcacgg attgaaccgg ctaatcgtta tgtggcagag aaattaagaa
   115861 taacgcccgg tcaggatatt ctttaccttg agcgtttacg ttcaattggt gatgaaaaag
   115921 cgatgctgat agagaaccgt atcaatattg agctatgccc cggcatcgtg gaaatagatt
   115981 ttaatcaaca caatttattt ccaacaatag aaagtttgtc gcaaagaaaa attcgttact
   116041 cggaaagtcg ctatgccgcg cgattaattg gtaatgaacg cggtcatttt ttagatatca
   116101 gtgaagatgc acccgttttg catctggagc agttagtctt tttctcccgc ggattacccg
   116161 ttgagtttgg caacgtctgg ttaaaaggaa ataaatacta tcttggcacc gtgctgcaac
   116221 ggcgggaagt gagttaagta cactttttta agaggaataa aatgcaaggc atacagtttc
   116281 aggaaaattt tattcaacga cttccggcag ggttaagcgt tgaacagatc atccgtcagt
   116341 tagcgcaacc gttggttacc gccgaactgg tggtccctga ttttgccgat catgtactgg
   116401 aacgtgaggc gacgtaccca acgggactgc ctaccgaacc accgtgtgtc gccattccgc
   116461 acacggacca taaacatgtc aggcacaatg caattgccgt cggcatcctg ccggaaccgg
   116521 tagtgtttgc cgatatgggc ggcgaccctg atcccgtacc tgtcagggtg atttttttgc
   116581 tcgccttagg cgaaagtaac aaacaattaa atgccctggg gtggattatg gagatgattc
   116641 aggatacgcc ctttatgtgc gccctgctta cgatggaaac aacagaaata cacacagcaa
   116701 tcttaaacaa aatgaaagaa cgaggtgaaa tatgagtcaa accattttat ttgtctgcgc
   116761 cacgggtatt gccacatcca cggcagtaac agaaaaagtc atggaatatt gtaaagagca
   116821 cggtcttaac gttaattatt ctcaaaccaa cgttgcctct ttacctggaa ataccgacgg
   116881 cgtagcactg gttgtttcaa cgactaaagt gccttatgaa ctcgacgtgc cggtggttaa
   116941 cggcttgcca attattactg gtattggcga agagaaagta ttggcgcaaa ttgtttctat
   117001 cctaaaaaag taattaataa taattaattt caacacacta catcaggcgc taacgcgagg
   117061 tgcgctctcg cctgaaaaag aggtttatta tgaacgatat cgcgcatacc ctctataata
   117121 ttgtgcaata tatattggga tttggtccca cggtaatgtt gccgttggtg ttatttattc
   117181 ttgctctctt ttttaaagta aaacccgcta aagccttacg ttcgtcatta acggtcggca
   117241 ttggttttgt cggtatttat gccattttcg atattcttac cagcaatgtc gggccagcgg
   117301 cccaggcgat ggttgaacgc accggaatta atttaccggt ggtggattta ggctggccgc
   117361 cgctttccgc tattacatgg ggttcgccaa ttgccccgtt tgttattccc ctgactattc
   117421 tgattaacgt ggcgatgctg gcgttaaata aaacccgcac cgttgacgta gatatgtgga
   117481 actactggca ttttgccctt gccggtacgc tggtttatta cagcaccggc agcctgttct
   117541 ttggtttgct ggcagcggcg attgctgcgg tggtagtact taaactcgcc gactggtctg
   117601 cgccactggt acaaaaatac tttggcctgg aagggatctc tttgccgacg ctctcttcgg
   117661 tggtgttctt tccggtcggt ctgttggtcg acaaaatcat cgaccatatc cccggcctca
   117721 atcgtattca tatcgatccg gaaaccgtac agaaaaagtt tggcattttc ggcgaaccga
   117781 tgatggttgg cactattctg ggcatttttc tcggcgtaat tgccggatac gatttcaaaa
   117841 aagtattgct gcttggcatc agcattggcg gtgtgatgtt catcctgcca cgcatggttc
   117901 gcattctgat ggaaggttta ttaccgctgt ctgaagccat taaaaagtat ctcaatgcca
   117961 aataccctga ccgtgacgat ctctatatcg gcctggatat cgccgttgcc gtaggtaatc
   118021 cggcgattat ctccaccgcc ctgctgctaa cgccaatctc ggtctttatc gcgtttgtcc
   118081 tcccaggtaa tgaagtcctg ccgcttggcg accttgccaa cctggcggta atggcgtcga
   118141 tgattgcttt agccagccgt ggcaatattt tccgcaccgt tctggcagcg attccggtca
   118201 ttattgccga cctgtggatt gctaccaaaa tcgcgccgtt tattaccgga atggcgaaag
   118261 acgttaactt caaatttacc gaaggctcca gcggccaggt ttccagtttc cttgatggcg
   118321 gtaacccgtt ccgcttctgg ctgctggaaa tcttcaacgg caatctcatc gccattgctc
   118381 tggtgccggt tattgccctg gtgctgtatg gcattttccg aatgacgcgg agcacggttt
   118441 atgcctgata acagcgccgc tatcgttatc gatattggca ccaccaattg caaagtcacc
   118501 tgcttttcct gcctggacgc aacgacgttg ggcgcgcata aattcgtgac ggcaaaacag
   118561 atctccccac agggcgatgt cgatttcgat atcgacgccc tctggcagga ggtccgccag
   118621 gcgatagcgc aactgaacgc cgcttcaccg ctgccagtca ggcggatcag cattgccagt
   118681 tttggcgagt caggggtgtt tcttgacgaa catggcgaga tcctgacgcc aatgctggca
   118741 tggtatgacc gtcgcggtga agagtatccg gcaacgctta gcgaggcaga cagtgcggca
   118801 ctttatgaca tttgcgggtt accactgcac agcaattact ctgccttcaa aatgcgttgg
   118861 ttgctggagc attatccgct gcgcaatcgt cgcggcctgc gctggctaca tgcaccggaa
   118921 gtgctgctct ggcggctgac tggcgaacag cgaacggata tcaccttagc cagccgtaca
   118981 ctgtgtctgg acgtgcgcaa aggcgaatgg tcagcgaaag cggcggcgtt gttacacgtt
   119041 ccctgttcag catttgcgcc attggtgcag ccaggcgaac acgccggatg ggtcagcgag
   119101 tcgctctgca aaacgcttgg gttctcgcaa ccggtcagcg tgacgctggc cggacatgac
   119161 catatggtgg gtgcgcgggc gttgcagatg atgccgggcg atatccttaa ctcgacgggc
   119221 accacggaag gcattctgca actggataca caaccgacgc tggatgaaca ggccaaacgt
   119281 gacaagctgg caaacggctg ttactcgctt gccaaccagt tcaccctgtt tgcttctctg
   119341 cccgtgggcg gtttcgcgct ggagtggctg cgcaacacgt tccggctaac tgatgaggag
   119401 atcgccgcgt cgctcaatcg tgggtatgcg gattacctgg cggggaactg gtcgcttgat
   119461 gacattcccg tctttattcc gcatcttcgc ggttcgggtt caccctataa aaatcgccac
   119521 acccgcggat tattttatgg gcttggcgat acgttaaata tcgacatgtt aatttccagc
   119581 gtctcactgg gattaaccat ggaatttgcc aactgctttg cctgttttaa tgtgcctggc
   119641 accagcgtgt taaaagtgat cggtccggcg gcccataacc ccctttggct gcaattaaag
   119701 gcagatattt tacagcgtcc ggttgaagca attgcattta gcgaggcggt ttctgtcgga
   119761 gccttattaa ccgcggcacc ggatattccg ccgccgcaag ttaccatagc ccaacgtttg
   119821 ttaccgaatc gggcaagata tcatcaatta cagcgttatc agcacaaatg ggaaagctgg
   119881 tatcagttga aattacagca agaaggcgtg atgccattac atcatcggga ggagcactat
   119941 gttgactaaa accgtggagg tcagaaacag tactggcctg catgcacgtc cggcggcctg
   120001 tctggcgaaa gccgccaaaa agtatagctg taaagtgacg ctgcattatg agggtaatga
   120061 tattaatgcc accagtatga tgaatattat gcgcgccgga ataaaaggcg gaaaaacggt
   120121 ggaaatacgc tgcgacggtg atgatgaaaa cgaggccata cagactctca ccgccttatt
   120181 tcgcgaccgc ttcggtgaag ctgaataatt aactgatatt aaaaaatgag gttttcaata
   120241 tgccattagt gaatggacgg atactgctcg accgtattca ggaaaaacgg gtattagcgg
   120301 gcgcgtttaa taccaccaat ctggagacga caatttcaat tttaaacgct attgagcgtt
   120361 cgggattacc caacttcatt cagattgcgc ccaccaatgc acaactgtcg ggatacgatt
   120421 acatatatga aatcgtgaaa cgtcacgccg ataaaatgga tgttccggtc agtcttcatc
   120481 tggatcacgg gaaaacccag gaggatgtta aacaggcggt gcgcgcgggc tttacctcgg
   120541 tcatgattga cggtgccgca ttctcttttg aagagaatat cgctttcacc caggaagctg
   120601 tcgatttctg taaatcttat ggcgtgccgg ttgaagcgga gctgggggcg attctcggca
   120661 aagaagatga tcacgtcagc gaggccgatt gcaaaacgga accggaaaag gtgaagacct
   120721 ttgtcgaacg caccggttgc gacatgctgg cagtttctat tggtaacgtt catggcctgg
   120781 acgatatacc gcggatcgat atccccctgc tgaagcgcat tgccgaagtc agcccggtgc
   120841 cgctggttat ccacggcggc tccggtatcg ccccggagat attacgcagt ttcgttaatt
   120901 atcgcgtcgc taaagtgaat atcgccagcg atctgcgcaa agcgtttatc accgcagtcg
   120961 gcaaagcgta tgtgaataat cacaacgagg cgaatctggc acgagtgatg gcatcagcaa
   121021 aaaacgccgt cgaggaagat gtgtatagca aaatcctgat gatgaatgag gggcatcggt
   121081 cggtaaaata aatcctcgcc ggatgcggcg tgacgcctta tccggcctac aaaaccacta
   121141 gaattcaata aattgcacca ccgcgtaggc ccgataagct tgcgtatcgg gcactgtatt
   121201 tacgccattg tcccaatcgt cttcctgaat cgcagcagcg caatggtaaa aaacgcgccg
   121261 ccaatcgcta tcagcgtcag gaactgcggc cagacgattt cgaatccggc accacgatag
   121321 aggatggcct gcgcgaggct gacaaagtgt gtcgtcggca tggtcagcat aatgtcctgc
   121381 accatctgcg gcatactttc gcgcggcgtg gaaccaccgg aaagcatttg cagcggcagc
   121441 agcaccagaa tcaccagcag ccccagttgc ggcattgaac gcgctatcgt ccccataaaa
   121501 atgccgattg acgtggtggc aaacagactg agcgccacgc ccagcataaa cagcgggatc
   121561 gagccttcta tcggtacgcc cagcacgcct ttcaccatca gcaccagcga taatcccgac
   121621 accaccagca ccaccagccc catcgaccag acctttgcca tcatgatctc aaacggcgtt
   121681 atcggcatca ccagtaagtg ttccaccgtg ccgtgttcac gctcgcggat cagcgccgat
   121741 ccggtcaata caatcgccag catggtaatg ttgttgatga tcgccatcac cccgccaaac
   121801 cacgcgggat caaggttcgg gttaaagcgc atccgtgttt ccagcgatac caacggttcg
   121861 ctgttatcac ggtagcgcgc gacaaagctg ttcacttcac cgttgataat attctggata
   121921 tacccattgc cggtaaatgc ctggctcatg cgcgtggcat caacgttcac ctgaatatcc
   121981 ggctggcgtc cggcgaggac atcacgctga aaattaggcg gaatatttat cgcgaaggta
   122041 taacgtccgg cgtccagccc ggcatccatc tcatcggcgg tgatcatctc cggtggcaaa
   122101 aaccacgggc gatagaagct gttaacgatc cggttcgata actgcgattg atccatatcg
   122161 gcaatggcga tcggcgcgag gttcaacgat cctggcgtaa cggtcgctga cgaatacacc
   122221 gacaccgtaa acgagaagac aatcagcgtc agcatcgctt tatcaccgag cagactgcgc
   122281 aactctttga tacccagatt aaaaatatta cgtaaatggc gcatcatccc tcctgttttt
   122341 tcagcagcag gatgcttaag cccatcacca gcggtatggc tatcagcaac gggataaaaa
   122401 gttgccacaa atcagtcaga tccagcgctt tcgagaacgt tccgcgggcg atagtcagaa
   122461 aatgactggt cgggtaaacc tcgccgatcc aacgtcctgg accttccagc gaagctaccg
   122521 gatcgatcat cccggaaaac tgtgtcgccg ggatcaacgt gataattgcc gtaccgaaaa
   122581 tggcggcaat ctggcttttc ataaaggtgg agatcagcag ccccattccg gtggcgatga
   122641 tgatatacag cagcgccgcc agggtgagcg tcaggaaact gcctttatgc ggcacaccaa
   122701 acacaaacac cgacagtgcg caaagcaaga aaaagttcag cattcccagt gcgatatacg
   122761 gtaactgttt gccgagcaaa aattcgctgc gggtggtggg ggtcacgtaa aggttgataa
   122821 tcgaccctag ctctttttcc cgcaccacgc taagggcgct tagcattgac gggatcatca
   122881 tcagcagaag cgggatcacc gccggaacaa tcgccggcag gctttttacg tccgggttat
   122941 agcgatagcg cgtctcaata ttcatcagcc cgctttggct ggcaggtgtg gactgtcggc
   123001 tcgccacatc ctgtagccag ctctggtgca tggcctgcac gtagcctttt accgtttcgg
   123061 cgcggctcgg cattgcaccg tcgatccaga caccgagttc cacgggcgta ccacgcgcga
   123121 tatcgcgccc gaaattggga gggatctcaa ttgccaccgt gatatcgccc gcacgcatcc
   123181 gacgatcaag ctcgtcataa ctggtgagcg gtggctgttc gataaagtaa cgggaaccgg
   123241 agagattgag cgtccacgcc tggctactga cggtttgatc gcggtcaagc accgcaaagc
   123301 gcaggttttc cacatccata ctgatgccgt aacccattat cagcatcagg atcaccgttc
   123361 ccatcagcgc cagcgtcgag cgtaccggat cgcggcgcag ttccagcgct tcacggcggc
   123421 tgtagctaaa cagacggcgc aggctgaatc cctgacgtgg cgcgtgggtg gtgtcgtgta
   123481 ccacgggcgg cgcttcagct tcgttgctct gccctgccgc ttcctgcaaa taggcgataa
   123541 atgcctcttc gagactggcg gctccgcgtt tctcaaccag ttccggcggt gtaccgctgg
   123601 cgagcacttt tccagcgtgc atcagtgaga tacggtcaca acgttgcgct tcgttcataa
   123661 agtgggtgga gatgaagata gtcactttgt cctgacgcga gagatccacc atcaactgcc
   123721 agaacatatc cctcgccacc ggatcgacgc cagaagtagg ctcatcgagg attaacatct
   123781 ccgggcgatg aatcaccgcc accgccagcg atagccgctg gcgaatgccg agcggcaatg
   123841 actccggcaa tacatcttcg acgtcgttga gcttaaaacg ctcgctcatt tcagccactc
   123901 gcgcgggaat ttccgcttcc gggatatgaa acagacgagc atgtaactcc aggttttgcc
   123961 gcacggtgag ttcgttatag agcgaaaacg cctgcgacat atagcccacc cggcgacgag
   124021 tatcgatatc ttttggatca accggttgcc cgaacagcca cgcctcacct tcgctggcgg
   124081 gcagcagtcc ggtaagcatt ttcatggtgg tggatttacc gcagccgttc gaaccaagaa
   124141 aaccaaaaat ctccccgcgt ggaatgcgga aattgacgtg atcaacggca acgaaggaac
   124201 caaaacgcat ggtcagatcg cgcgcttcga tggcaatctc tgcgttttca ggttgatacg
   124261 gtgggatcac taccgcctga tgcgcctggc gttgtgcttg cggtaacaga tttataaatg
   124321 cttcttccag cgtggcgctt tgcgtttgct gccgcaactc ttcggcgcta ccggttgcca
   124381 gcacttctcc ggcattcatc gctaccagcc agtcgaagcg ttcggcctct tccatgtagg
   124441 cggtggcaac cagcacgctc atattgctct gccgctggcg aatactgtcg atcagatccc
   124501 agaactgggc gcgagagagc gggtcaaccc ccgttgttgg ctcatcaaga atcaacaatt
   124561 ccgggtcgtg gattaacgca cagcacagcc caagtttttg cttcatcccg ccggagagtt
   124621 tccctgccgg acgatcgcga aacggtgcta atccggtgct ggccagcagc tcattaattc
   124681 gcacttcccg ctccgctttg tcgtgaccga acaggcgggc gaaaaaatcg acgttttcat
   124741 acaccgacaa ggtgtggtag agatttttgc ccagcccctg cggcatccag gcgatgcgcg
   124801 ggcagacgtc gcggcgatgc ttcgggtcgc gcatatcgcc gcccagcacc atcacgccgc
   124861 cctgctcaat gacgcgagca ccggaaatca acgacaacaa gctcgattta ccgacaccat
   124921 ccggtccaat cagcccgacc atgcaacggg ccggaatatc gagtgtgata ttgttcagcg
   124981 caacggtttt tccataatgc tggctcacgc ccgccagttg cgcgacaggc gggacgggaa
   125041 ccagttccag atgcatcatt gcggcaacct caccaccagg tcgtcaggcc acggaagttc
   125101 ttcattcacc cgcacccacg ctacgcccgg caatccggtt ttgacatatt ccagatgctg
   125161 ctggagtaat tccggtggga tacgcgcttt gacacggaac atcaatttca gccgctcatc
   125221 gctggtttcg acagtttttg gcgtgaactg ggcgacgctg gcgacaaaac tgatggtcgc
   125281 gggaatacgc agatctggtg cagcatcaag aatcaaacgt gcttcaccgc ccagtttcag
   125341 cgtgcccgcc tgttcggttg gcagaaagaa ggtcatatag acgtcgctga gatcgaccat
   125401 attcagcacc cgaccgcctg ctgccagcac ttcgcctggc tcggcaaccc gatactgcac
   125461 gcgtccgtca cgcggggctt tcagttcgct gtcatcgata tctgcggcaa tgcgccgttc
   125521 agtggcttgt gccgcttcga cgcgagtttg cgcctgaatg atattggtgc gtgccgcctc
   125581 tatagcggct ttagaagcag aaacctgagc ttttgccgat tccagcgcag ctcgggcgct
   125641 ctcagcggcg gcgcggtcgt catccaactg ttgcgcagaa attgcccccc gtcgtgccag
   125701 tgagcgggaa cgcgtatgac gttttgctac ggagtccagt tctgcctggc gttgattaac
   125761 cagcgactgt gcggcacgag tttcgctttg gcgttgctcc agcaaagcct gcgcggcggc
   125821 aacggcactt tgtgcttctt tgatttgcgc gatggcttcc agtcgctgtt cctgcaacac
   125881 gcgagtatcc atcttcgcca gcacttcacc ttctcgaaca aactggcctt ctttcaccag
   125941 aatggtgtcg atacgcccgg caattttgct ggcgatatcc acttccgtcg cttcaatgcg
   126001 cccattgctg acagcaaagc cttccggcac acccgccggg cgcaacagcc accacgccac
   126061 gacggcagcc accgccagtg ccccgacacc ccaccacgcc agatggcgct tactcttatc
   126121 cataatcgac ccgccataat ccctgtaagc aatcgacacg tgttacaact gccaccgaac
   126181 gacgttcagc ggattcttcc atccgggata agaaggaaat ggcaaatacg accagcgcac
   126241 agagcattga gcgccaatgg ggaaggctta gtgaaaacgg gtagtaatgt tgtcatcatc
   126301 agcattcctg gccgtaaatg aaacacgcat actcataagt gaaatcaatt aaatactaac
   126361 agttatattc atgttgtcta tttgattgcc atcaacttgt gatacatcgc gatgttcttc
   126421 cgttcgctgg tgttttttcc aaaaatattc tgtacaggtt cacagattct gttgcacagg
   126481 tttccgtaac agggaaacct gatgactatc acatagcgtt attatcgcct gatgtgcgaa
   126541 ta
//
LOCUS       DAHHTG010000002        97007 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.002, whole genome shotgun sequence.
ACCESSION   DAHHTG010000002 DAHHTG010000000
VERSION     DAHHTG010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 97007)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 97007)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..97007
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.002"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            245..1816
                     /gene="garD"
                     /locus_tag="NBI74_000118"
     CDS             245..1816
                     /gene="garD"
                     /locus_tag="NBI74_000118"
                     /EC_number="4.2.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate dehydratase"
                     /protein_id="HCD1930503.1"
                     /translation="MANIEIRQETPTAFYIKVHDTDNVAIIVNDNGLKAGTRFPDGLE
                     LIEHIPQGHKVALLDIPANGEIIRYGEVIGYAVRAIPRGSWIDESMVVLPEAPPLHTL
                     PLATKVPEPLPPLEGYTFEGYRNADGSVGTKNLLGITTSVHCVAGVVDYVVKIIERDL
                     LPKYPNVDGVVGLNHLYGCGVAINAPAAVVPIRTIHNISLNPNFGGEVMVIGLGCEKL
                     QPERLLTGTDDVQAIPVESASIVSLQDEKHVGFQSMVEDILQVAERHLQKLNQRQRET
                     CPASELVVGMQCGGSDAFSGVTANPAVGYASDLLVRCGATVMFSEVTEVRDAIHLLTP
                     RAVNEEVGKRLLEEMEWYDNYLNMGKTDRSANPSPGNKKGGLANVVEKALGSIAKSGK
                     SAIVEVLSPGQRPTKRGLIYAATPASDFVCGTQQVASGITVQVFTTGRGTPYGLMAVP
                     VIKMATRTELANRWFDLMDINAGTIATGEETIEEVGWKLFHFILDVASGKKKTFSDQW
                     GLHNQLAVFNPAPVT"
     gene            1965..2300
                     /gene="prlF"
                     /locus_tag="NBI74_000119"
     CDS             1965..2300
                     /gene="prlF"
                     /locus_tag="NBI74_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin PrlF"
                     /protein_id="HCD1930504.1"
                     /translation="MPANARSNAVLTTESKVTIRGQTTIPAPVREALKLKPGLDSIHY
                     EILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNNPQKTRPFDIQQGKKLVAGMDVN
                     IDDEIGDDE"
     gene            2300..2764
                     /gene="yhaV"
                     /locus_tag="NBI74_000120"
     CDS             2300..2764
                     /gene="yhaV"
                     /locus_tag="NBI74_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ribonuclease
                     toxin YhaV"
                     /protein_id="HCD1930505.1"
                     /translation="MDFPQRVNGWALYAHPCFQETYDALVAEVEALKGKDPENYQRKA
                     ATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKNKDWSRVKFGAGRYRLFFRYSEK
                     EKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEENH"
     gene            complement(2819..3628)
                     /gene="agaR"
                     /locus_tag="NBI74_000121"
     CDS             complement(2819..3628)
                     /gene="agaR"
                     /locus_tag="NBI74_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aga operon transcriptional regulator AgaR"
                     /protein_id="HCD1930506.1"
                     /translation="MSNTDASGEKRVTGTSERREQIIQRLRQQGSVQVNDLSALYGVS
                     TVTIRNDLAFLEKQGIAVRAYGGALICDSTTPSVEPSVEDKSALNTAMKRSVAKAAVE
                     LIQPGHRVILDSGTTTFEIARLMRKHTDVIAMTNGMNVANALLEAEGVELLMTGGHLR
                     RQSQSFYGDQAEQSLQNYHFDMLFLGVDAIDLERGVSTHNEDEARLNRRMCEVAERII
                     VVTDSSKFNRSSLHKIIDTQRIDMIIVDEGIPADSLEGLRKAGVEVILVGE"
     gene            3877..5157
                     /gene="kbaZ"
                     /locus_tag="NBI74_000122"
     CDS             3877..5157
                     /gene="kbaZ"
                     /locus_tag="NBI74_000122"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit KbaZ"
                     /protein_id="HCD1930507.1"
                     /translation="MKHLTEMVRQHKAGKTNGIYAVCSAHPLVLEAAIRYASANQTPL
                     LIEATSNQVDQFGGYTGMTPAGFRGFVCQLADSLNFPQDALILGGDHLGPNRWQNLPA
                     AQAMANADDLIKSYVAAGFKKIHLDCSMSCQDDPIPLTDGIVAERAARLAKVAEETCR
                     EHFGEADLEYVIGTEVPVPGGAHETLSELAVTTPDAARATLEAHRHAFEKQGLNAIWP
                     RIIALVVQPGVEFDHTNVIDYQPAKATALSQMVENYETLIFEAHSTDYQTPQSLRQLV
                     IDHFAILKVGPALTFALREALFSLAAIEEELVPAKACSGLRQVLENVMLDRPEYWQSH
                     YHGDGNARRLARGYSYSDRVRYYWPDSQIDDAFAHLVRNLADSPIPLPLISQYLPLQY
                     VKVRSGELQPTPRELIINHIQDILAQYHTACEGQ"
     gene            5180..5653
                     /gene="agaV"
                     /locus_tag="NBI74_000123"
     CDS             5180..5653
                     /gene="agaV"
                     /locus_tag="NBI74_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIB"
                     /protein_id="HCD1930508.1"
                     /translation="MPNIVLSRIDERLIHGQVGVQWVGFAGANLVLVANDEVAEDPVQ
                     QNLMEMVLAEGIAVRFWTLQKVIDNIHRAADRQKILLVCKTPADFLTLVKGGVPVNRI
                     NVGNMHYANGKQQIAKTVSVDAGDIAAFNDLKAAGVECFVQGVPTEPAVDLFKLL"
     gene            5664..6443
                     /locus_tag="NBI74_000124"
     CDS             5664..6443
                     /locus_tag="NBI74_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HCD1930509.1"
                     /translation="MEISLLQAFALGIIAFIAGLDMFNGLTHMHRPVVLGPLVGLVLG
                     DLHTGILTGGTLELVWMGLAPLAGAQPPNVIIGTIVGTAFAITTGVKPDVAVGVAVPF
                     AVAVQMGITFLFSVMSGVMSRCDRMAENADTRGIERVNYLALLALGTFYFLCAFLPIY
                     FGAEHAKTIIDVLPQRLIDGLGVAGGIMPAIGFAVLLKIMMKNVYIPYFILGFVAAAW
                     LKLPVLAIAAAALAMALIDLLRKSPEPTQPAAQKEEFEDGI"
     gene            6433..7311
                     /locus_tag="NBI74_000125"
     CDS             6433..7311
                     /locus_tag="NBI74_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019079236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HCD1930510.1"
                     /translation="MASNQTTLPNVSENEETLLTGVNENVYEDQSIGAELTKKDINRV
                     AWRSMLLQASFNYERMQASGWLYGLLPALKKIHTNKRDLARAMKGHMGFFNTHPFLVT
                     FVIGIILAMERSKQDVNSIQSTKIAVGAPLGGIGDAMFWLTLLPICGGIGASLALQGS
                     ILGAVVFIVLFNVVHLGLRFGLAHYAYRMGVAAIPLIKANTKKVGHAASIVGMTVIGA
                     LVATYVRLSTTLEITAGDAVVKLQADVIDKLMPAFLPLVYTLTMFWLVRRGWSPLRLI
                     AVTVVLGIVGKFCHFL"
     gene            7329..7763
                     /locus_tag="NBI74_000126"
     CDS             7329..7763
                     /locus_tag="NBI74_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HCD1930511.1"
                     /translation="MLSIILTGHGGFASGMEKAMKQILGEQSQFIAIDFPETSSTALL
                     TSQLEEAIAQLDCEDGIVFLTDLLGGTPFRVASTLAMQKPGCEVITGTNLQLLLEMVL
                     EREGLSGEEFRVQALECGHRGLTSLVDELGRCHEECPVEEGI"
     gene            7760..8893
                     /gene="nagA"
                     /locus_tag="NBI74_000127"
     CDS             7760..8893
                     /gene="nagA"
                     /locus_tag="NBI74_000127"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HCD1930512.1"
                     /translation="MTHVLRARRLLTEEGWLDDHQLRIADGVIAAIEPIPVGVTERDA
                     ELLCPAYIDTHVHGGAGVDVMDDAPDALDKLAMHKAREGVGSWLPTTVTAPLNTIHAA
                     LKRIALRCQRGGPGAQVLGSYLEGPYFTPQNKGAHPPELFRELEIAELDQLIAVSQQT
                     LRVVALAPEKEGALQAIRHLKQQNVRVMLGHSAATWQQTRAAFDAGADGLVHCYNGMT
                     GLHHREPGMVGAGLTDKRAWLELIADGHHVHPAAMSLCCCCAKERIVLITDAMQAAGM
                     PDGHYTLCGEEVQMHGGIVRTAFGGLAGSTLSVDAAVRNMVELTGVTPAEAIHMASLH
                     PARMLGVDGVLGSLKPGKRASVVALDSGLHVQQIWIQGQLASF"
     gene            9243..10397
                     /locus_tag="NBI74_000128"
     CDS             9243..10397
                     /locus_tag="NBI74_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AgaS family sugar isomerase"
                     /protein_id="HCD1930513.1"
                     /translation="MPENYTPAAAATGTWTEEEIRHQPRAWIRSLTNIDALRSALNNF
                     LEPLLRKENLQIILTGAGTSAFIGDIIAPWLASHTGKNFSAVPTTDLVTNPMDYLNPA
                     HPLLLISFGRSGNSPESVAAVELANQFVPECYHLPITCNEAGALYQNAINSDNAFALL
                     MPAETHDRGFAMTSSITTMMASCLAVFAPETINSQTFRDVADRCQAILTSLGDFSEGV
                     FGYAPWKRIVYLGSGGLQGAARESALKVLELTAGKLAAFYDSPTGFRHGPKSLVDDET
                     LVVVFVSSHPYTRQYDLDLLAELRRDNQAMRVIAITAESNDVIAAGPHIILPPSRHFI
                     DVEQAFCFLMYAQTFALMQSLHMGNTPDTPSASGTVNRVVQGVTIHPWQA"
     gene            10410..11270
                     /gene="kbaY"
                     /locus_tag="NBI74_000129"
     CDS             10410..11270
                     /gene="kbaY"
                     /locus_tag="NBI74_000129"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit KbaY"
                     /protein_id="HCD1930514.1"
                     /translation="MSIISTKYLLQDAQANGYAVPAFNIHNAETIQAILEVCSEMRSP
                     VILAGTPGTFKHIALEEIYALCSAYSTTYNMPLALHLDHHESLDDIRRKVHAGVRSAM
                     IDGSHFPFAENVKLVKSVVDFCHSRDCSVEAELGRLGGVEDDMSVDAESAFLTDPQEA
                     KRFVELTGVDSLAVAIGTAHGLYSKTPKIDFQRLAEIREVVDVPLVLHGASDVPDEFV
                     RRTIELGVTKVNVATELKIAFAGAVKAWFAENPQGNDPRYYMRVGMDAMKEVVRNKIN
                     VCGSANRISA"
     gene            11437..11913
                     /gene="agaB"
                     /locus_tag="NBI74_000130"
     CDS             11437..11913
                     /gene="agaB"
                     /locus_tag="NBI74_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIB"
                     /protein_id="HCD1930515.1"
                     /translation="MSSPNILLTRIDNRLVHGQVGVTWTSTIGANLLVVVDDVVANDD
                     IQQKLMGITAETYGFGIRFFTIEKTINVIGKAAPHQKIFLICRTPQTVRKLVEGGIDL
                     KDVNVGNMHFSEGKKQISSKVYVDDQDLTDLRYIKQRGVNVFIQDVPGDQKEQIPD"
     gene            11952..12755
                     /gene="agaC"
                     /locus_tag="NBI74_000131"
     CDS             11952..12755
                     /gene="agaC"
                     /locus_tag="NBI74_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIC"
                     /protein_id="HCD1930516.1"
                     /translation="MHEITLLQGLSLAALVFVLGIDFWLEALFLFRPIIVCTLTGAIL
                     GDIQTGLITGGLTELAFAGLTPAGGVQPPNPIMAGLMTTVIAWSTGVDAKTAIGLGLP
                     FSLLMQYVILFFYSAFSLFMTKADKCAKEADTAAFSRLNWTTMLIVASAYAVIAFLCT
                     YLAQGAMQALVKAMPAWLTHGFEVAGGILPAVGFGLLLRVMFKAQYIPYLIAGFLFVC
                     YIQVSNLLPVAVLGAGFAVYEFFNAKSRQQAQPQPVASKNEEEDYSNGI"
     gene            12745..13536
                     /gene="agaD"
                     /locus_tag="NBI74_000132"
     CDS             12745..13536
                     /gene="agaD"
                     /locus_tag="NBI74_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IID"
                     /protein_id="HCD1930517.1"
                     /translation="MGSEISKKDITRLGFRSSLLQASFNYERMQAGGFTRAMLPILKK
                     IYKDDKPGLSAAMKDNLEFINTHPNLVGFLMGLLISMEEKGENRDTIKGLKVALFGPI
                     AGIGDAIFWFTLLPIMAGICSSFASQGNLLGPILFFAVYLLIFFLRVGWTHVGYSVGV
                     KAIDKVRENSQMIARSATILGITVIGGLIASYVHINVVTSFAIDSTHSVALQQDFFDK
                     VFPNILPMAYTLLMYYFLRVKKAHPVLLIGVTFVLSIVCSAFGIL"
     gene            13537..14304
                     /locus_tag="NBI74_000133"
     CDS             13537..14304
                     /locus_tag="NBI74_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019843039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactosamine-6-phosphate isomerase"
                     /protein_id="HCD1930518.1"
                     /translation="MERGTASGGASLLKEFHPVQTLQQVENYTALSERASEYLLAVIR
                     SKPDAVICLATGATPLLTYHYLVEKIHQQQVDVSQLTFVKLDEWVDLPLTMPGTCETF
                     LQQHIVQPLGLREDQLISFHSEEINETECERVTNLIARKGGLDLCVLGLGKNGHLGLN
                     EPGESLQPACHISQLDARTQQHEMLKTADRPVTRGITLGLKDILNAREVLLLITGEGK
                     QDATERFLTAKVSTAIPASFLWLHNHFTCVIDEMCRR"
     gene            complement(14354..15217)
                     /gene="rsmI"
                     /locus_tag="NBI74_000134"
     CDS             complement(14354..15217)
                     /gene="rsmI"
                     /locus_tag="NBI74_000134"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000809248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HCD1930519.1"
                     /translation="MKQHQSADNSQGQLYIVPTPIGNLADITQRALEVLQAVDLIAAE
                     DTRHTGLLLQHFGINARLFALHDHNEQQKAETLLAKLQEGQNIALVSDAGTPLINDPG
                     YHLVRTCREAGIRVVPLPGPCAAITALSAAGLPSDRFCYEGFLPAKSKGRRDALKAIE
                     AEPRTLIFYESTHRLLDSLEDIVAVLGESRYVVLARELTKTWETIHGAPVGELLAWVK
                     EDENRRKGEMVLIVEGHKAQEDDLPADALRTLALLQAELPLKKAAALAAEIHGVKKNA
                     LYKYALEQQGE"
     gene            15282..17318
                     /gene="lpoA"
                     /locus_tag="NBI74_000135"
     CDS             15282..17318
                     /gene="lpoA"
                     /locus_tag="NBI74_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoA"
                     /protein_id="HCD1930520.1"
                     /translation="MVPSTFSRLKAARCLPVVLAALIFAGCGTHTPDQSTAYMQGTAQ
                     ADSAFYLQQMQQSSDDTRINWQLLAIRALVKEGKTGQAVELVNQLPQELNDNQRREKT
                     LLAAEIKLAQKDFAGAQNLLAKITPADLEQNQQARYWQAKIDASQGRPSIDLLRALIA
                     QEPLLGAKEKQQNIDATWQALSSMTQEQANTLVINADENILQGWLDLQRVWFDNRNDP
                     DMMKAGIADWQKRYPNNPGAKMLPTQLVNVKAFKPASTNKIALLLPLNGQAAVFGRTI
                     QQGFEAAKNIGTQPVAAQVAAAPAADVAEQPQPQTVDGVASPAQASVSDLTGEQPAAQ
                     PVPVSAPATSTAAVNAPANPSAELKIYDTSSQPLSQILSQVQQDGASIVVGPLLKNNV
                     EELLKSNTPLNVLALNQPENIENRVNICYFALSPEDEARDAARHIRDQGKQAPLVLIP
                     RSSLGDRVANAFAQEWQKLGGGTVLQQKFGSTSELRAGVNGGSGIALTGSPITPRATT
                     DSGMTTNNPTLQTTPTDDQFTNNGGRVDAVYIVATPGEIAFIKPMIAMRNGSQSGATL
                     YASSRSAQGTAGPDFRLEMEGLQYSEIPMLAGGNLPLMQQALSAVNNDYSLARMYAMG
                     VDAWSLANHFSQMRQVQGFEINGNTGSLTASPDCVINRKLSWLQYQQGQVVPAS"
     gene            17276..17671
                     /locus_tag="NBI74_000136"
     CDS             17276..17671
                     /locus_tag="NBI74_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HCD1930521.1"
                     /translation="MATVPTRSGSPRQLTTKQTGDAWEAQARRWLEGKGLRFIAANVN
                     ERGGEIDLIMREGRTTVFIEVRYRRSALYGGAAASVTRSKQHKLLQTARLWLARHNGS
                     FDTVDCRFDVVAFTGNEVEWIKDAFNDHS"
     gene            17691..18281
                     /gene="diaA"
                     /locus_tag="NBI74_000137"
     CDS             17691..18281
                     /gene="diaA"
                     /locus_tag="NBI74_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001483830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA initiator-associating protein DiaA"
                     /protein_id="HCD1930522.1"
                     /translation="MQERIKACFTESIQTQIAAAEALPDAISRAAMTLVQSLLNGNKI
                     LCCGNGTSAANAQHFAASMINRFETERPSLPAIALNTDNVVLTAIANDRLHDEVYAKQ
                     VRALGHAGDVLLAISTRGNSRDIVKAVEAAVTRDMTIVALTGYDGGELAGLLGPQDVE
                     IRIPSHRSARIQEMHMLTVNCLCDLIDNTLFPHQDV"
     gene            18291..18866
                     /gene="yraP"
                     /locus_tag="NBI74_000138"
     CDS             18291..18866
                     /gene="yraP"
                     /locus_tag="NBI74_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divisome-associated lipoprotein YraP"
                     /protein_id="HCD1930523.1"
                     /translation="MKALSPIAVLISALLLQGCVAAAVVGTAAVGTKAATDPRSVGTQ
                     VDDGTLEVRVNSALSKDEQIKKEARINVTAYQGKVLLVGQSPNAELSARAKQIAMGVD
                     GANEVYNEIRQGQPIGLGEASNDTWITTKVRSQLLTSDLVKSSNVKVTTENGEVFLMG
                     LVTEREAKAAADIASRVSGVKRVTTAFTFIK"
     gene            complement(18979..20019)
                     /locus_tag="NBI74_000139"
     CDS             complement(18979..20019)
                     /locus_tag="NBI74_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001521390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HCD1930524.1"
                     /translation="MTGQSSSQAATPIQWWKPALFFLVVIAGLWYVKWQPYYGKAFTA
                     AETHSIGKSILAQADANPWQAALDYAMIYFLAVWKAAVLGVILGSLIQVLIPRDWLLR
                     TLGQSRFRGTLLGTLFSLPGMMCTCCAAPVAAGMRRQQVSMGGALAFWMGNPLLNPAT
                     LVFMGFVLGWGFAAIRLVAGLVMVLLIATLVQKWVRETPQTQAPVEIDIPEAQGGFFS
                     RWGRALWTLFWSTIPVYILAVLVLGAARVWLFPHADGAVDNSLMWVVAMAVAGCLFVI
                     PTAAEIPIVQTMMLAGMGTAPALALLMTLPAVSLPSLIMLRKAFPAKALWLTGAMVAV
                     SGVIVGGLALLV"
     gene            complement(20092..20727)
                     /locus_tag="NBI74_000140"
     CDS             complement(20092..20727)
                     /locus_tag="NBI74_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312060.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HCD1930525.1"
                     /translation="MSQVLITGATGLVGGHLLRMLINEPKVNVIAAPTRRPLGDMPGV
                     FNPHDPQLTDALAQVTDPIDIVFCCLGTTRREAGSKEAFIHADYTLVVDTALTGRRLG
                     AQHMLVVSAMGANAHSPFFYNRVKGEMEEALIAQNWPKLTIARPSMLLGDRSKQRMNE
                     TLFAPLFRLLPGNWKSIDARDVARVMLAEAMRPEHEGVTILSSSELRKRAE"
     gene            20855..21373
                     /gene="yhbO"
                     /locus_tag="NBI74_000141"
     CDS             20855..21373
                     /gene="yhbO"
                     /locus_tag="NBI74_000141"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein/nucleic acid deglycase"
                     /protein_id="HCD1930526.1"
                     /translation="MSKKIAALITDEFEDSEFTSPADEFRKAGHEVITIEKQAGKTVK
                     GKKGEASVVIDKSIDEVTPAEFDALLLPGGHSPDYLRGDNRFVTFTRDFVNSGKPVFA
                     ICHGPQLLISADVIRGRKLTAVKPIVIDVKNAGAEFYDQEVVVDKDQLVTSRTPDDLP
                     AFNREALRLLGA"
     gene            complement(21353..21796)
                     /locus_tag="NBI74_000142"
     CDS             complement(21353..21796)
                     /locus_tag="NBI74_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbP family protein"
                     /protein_id="HCD1930527.1"
                     /translation="METLTAISRWLAKQHVVTWCVQQEGELWCANAFYLFDAQKVAFY
                     ILTEEKTRHAQMSGPQAAIAGTVNGQPKTVALIRGVQFKGEIRRLEGEESDLARKAYN
                     RRFPVARMLSAPVWEIRLDEIKFTDNTLGFGKKMIWLRNSGTEQA"
     gene            21847..22149
                     /gene="yhbQ"
                     /locus_tag="NBI74_000143"
     CDS             21847..22149
                     /gene="yhbQ"
                     /locus_tag="NBI74_000143"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage response exodeoxyribonuclease YhbQ"
                     /protein_id="HCD1930528.1"
                     /translation="MTPWFLYLIRTADNKLYTGITTDVERRYQQHQSGKGAKALRGKG
                     ELTLAFSAPVGDRSLALRAEYRVKQLTKRQKERLVTEGAGFAELLSSLQPPEIKSD"
     gene            complement(22136..22639)
                     /locus_tag="NBI74_000144"
     CDS             complement(22136..22639)
                     /locus_tag="NBI74_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HCD1930529.1"
                     /translation="MLIRVEIPIDAPGIDALLRRSFESDAEAKLVHDLREDGFLTLGL
                     VATDDEGQVIGYVAFSPVDVQGEDLQWVGMAPLAVDEKYRGQGLARQLVYEGLDSLNE
                     FGYAAVVTLGDPALYSRFGFELAAHHDLHCRWPGTESAFQVHRLADDALNGVTGLVEY
                     HEHFNRF"
     gene            complement(22633..23157)
                     /locus_tag="NBI74_000145"
     CDS             complement(22633..23157)
                     /locus_tag="NBI74_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 domain-containing protein"
                     /protein_id="HCD1930530.1"
                     /translation="MLDKLRSRIVHLGPSLLSVPVKLTPFALKRQVLEQVLSWQFRQA
                     LDDGELEFLEGRWLSIHVRDIDLQWFTSVVNGKLVVSQNAQADVSFSADASDLLMIAA
                     RKQDPDTLFFQRRLVIEGDTELGLYVKNLMDAIELEQMPKALRMMLLQLADFVEAGMK
                     TAPETKQTSVGEPC"
     gene            23366..24361
                     /locus_tag="NBI74_000146"
     CDS             23366..24361
                     /locus_tag="NBI74_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017457364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HCD1930531.1"
                     /translation="MELLCPAGNLPALKAAIENGADAVYIGLKDDTNARHFAGLNFTE
                     KKLQEAVSFVHQHRRKLHIAINTFAHPDGYARWQHAVDMAAQLGADALILADLAMLEY
                     AAERYPHIERHVSVQASATNEEAINFYHRHFDVARVVLPRVLSIHQVKQLARVTPVPL
                     EVFAFGSLCIMSEGRCYLSSYLTGESPNTVGACSPARFVRWQQTPQGLESRLNEVLID
                     RYQDGENAGYPTLCKGRYLVDGERYHALEEPTSLNTLELLPELMAANIASVKIEGRQR
                     SPAYVSQVAKVWRQAIDRCKADPQNFVPQSAWMETLGSMSEGTQTTLGAYHRKWQ"
     gene            24370..25248
                     /locus_tag="NBI74_000147"
     CDS             24370..25248
                     /locus_tag="NBI74_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HCD1930532.1"
                     /translation="MKYSLGPVLWYWPKETLEDFYQQAAASSADVIYLGEAVCSKRRA
                     TKVGDWLEMAKSLAGSGKQIVLSTLALVQASSELGELKRYVENGEFLIEASDLGVVNM
                     CAERKLPFVAGHALNCYNAVTLKILLKQGMMRWCMPVELSRDWLVNLLNQCDELGIRN
                     QFEVEVLSYGHLPLAYSARCFTARSEDRPKDECETCCIKYPNGRNVLSQENQQVFVLN
                     GIQTMSGYVYNLGNELASMQGLVDVVRLSPQGTDTFAMLDAFRANENGAAPLPLTANS
                     DCNGYWRRLAGLELQA"
     gene            25329..26336
                     /locus_tag="NBI74_000148"
     CDS             25329..26336
                     /locus_tag="NBI74_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="luciferase-like monooxygenase"
                     /protein_id="HCD1930533.1"
                     /translation="MTDKTIAFSLLDLAPIPEGSSAREAFSHSLDLARLAEKRGYHRY
                     WLAEHHNMTGIASAATSVLIGYLAANTTTLHLGSGGVMLPNHSPLVIAEQFGTLNTLY
                     PGRIDLGLGRAPGSDQRTMMALRRHMSGDIDNFPRDVAELVDWFDARDPNPHVRPVPG
                     YGEKIPVWLLGSSLYSAQLAAQLGLPFAFASHFAPDMLFQALHLYRSNFKPSARLEKP
                     YAMVCINIIAADSNRDAEFLFTSMQQAFVKLRRGETGQLPPPIQNMDQFWSPSEQYGV
                     QQALSMSLVGDKAKVRHGLQSILRETDADEIMVNGQIFDHQARLHSFELAMDVKEELL
                     G"
     gene            complement(26454..27698)
                     /gene="mtr"
                     /locus_tag="NBI74_000149"
     CDS             complement(26454..27698)
                     /gene="mtr"
                     /locus_tag="NBI74_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan permease"
                     /protein_id="HCD1930534.1"
                     /translation="MATLTTTQTSPSLLGGVVIIGGTIIGAGMFSLPVVMSGAWFFWS
                     MAALIFTWFCMLHSGLMILEANLNYRIGSSFDTITKDLLGKGWNVVNGISIAFVLYIL
                     TYAYISASGSILHHTFAEMSLNVPARAAGFGFALLVAFVVWLSTKAVSRMTAIVLGAK
                     VITFFLTFGSLLGHVQPATLFNVAESNASYAPYLLMTLPFCLASFGYHGNVPSLMKYY
                     GKDPKTIVKCLVYGTLMALALYTIWLLATMGNIPRPEFIGIAEKGGNIDVLVQALSGV
                     LNSRSLDLLLVVFSNFAVASSFLGVTLGLFDYLADLFGFDDSAVGRLKTALLTFAPPV
                     VGGLLFPNGFLYAIGYAGLAATIWAAIVPALLARASRKRFGSPKFRVWGGKPMIVLIL
                     VFGVGNALVHILSSFNVLPVYQ"
     gene            complement(27852..29741)
                     /gene="deaD"
                     /locus_tag="NBI74_000150"
     CDS             complement(27852..29741)
                     /gene="deaD"
                     /locus_tag="NBI74_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417631.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DeaD"
                     /protein_id="HCD1930535.1"
                     /translation="MAEFETTFADLGLKAPILEALNDLGYEKPSPIQAECIPHLLNGR
                     DVLGMAQTGSGKTAAFSLPLLQNLDPELKAPQILVLAPTRELAVQVAEAMTDFSKHMR
                     GVNVVALYGGQRYDVQLRALRQGPQIVVGTPGRLLDHLKRGTLDLSKLSGLVLDEADE
                     MLRMGFIEDVETIMAQIPEGHQTALFSATMPEAIRRITRRFMKEPQEVRIQSSVTTRP
                     DISQSYWTVWGMRKNEALVRFLEAEDFDAAIIFVRTKNATLEVAEALERNGYNSAALN
                     GDMNQALREQTLERLKDGRLDILIATDVAARGLDVERISLVVNYDIPMDSESYVHRIG
                     RTGRAGRAGRALLFVENRERRLLRNIERTMKLTIPEVELPNAELLGKRRLEKFAAKVQ
                     QQLESSDLDQYRALLSKIQPTAEGEELDLETLAAALLKMAQGERTLIVPPDAPMRPKR
                     EFRDRDDRGPRDRNDRGPRGDREDRPRRERRDVGDMQLYRIEVGRDDGVEVRHIVGAI
                     ANEGDISSRYIGNIKLFASHSTIELPKGMPGEVLQHFTRTRILNKPMNMQLLGDAQPH
                     TGGERRGGGRGFGGERREGGRNFSGERREGGRGDGRRFSGERREGRAPRRDDSTGRRR
                     FGGDA"
     gene            complement(29734..29814)
                     /gene="yrbN"
                     /locus_tag="NBI74_000151"
     CDS             complement(29734..29814)
                     /gene="yrbN"
                     /locus_tag="NBI74_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YrbN"
                     /protein_id="HCD1930536.1"
                     /translation="MKIADQFHDELCRLAAINFEAHVLHG"
     gene            complement(29921..30805)
                     /gene="nlpI"
                     /locus_tag="NBI74_000152"
     CDS             complement(29921..30805)
                     /gene="nlpI"
                     /locus_tag="NBI74_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020802045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpI"
                     /protein_id="HCD1930537.1"
                     /translation="MKPFLRWCFVATALTLAGCSNTSWRKSEVLAVPLQPTLQQEVIL
                     ARMEQILASRALTDDERAQLLYERGVLYDSLGLRALARNDFSQALAIRPDMPEVFNYL
                     GIYLTQAGNFDAAYEAFDSVLELDPTYNYAHLNRGIALYYGGRDKLAQDDLLAFYQDD
                     PNDPFRSLWLYLAEQKLDEKQAKEVLKQHFEKSDKEQWGWNIVEFYLGNISEQTLMER
                     LKADATDNTSLAEHLSETNFYLGKYYLSLGDLDSATALFKLAVANNVHNFVEHRYALL
                     ELSLLGQDQDDLAESDQQ"
     gene            complement(30914..33049)
                     /gene="pnp"
                     /locus_tag="NBI74_000153"
     CDS             complement(30914..33049)
                     /gene="pnp"
                     /locus_tag="NBI74_000153"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HCD1930538.1"
                     /translation="MLNPIVRKFQYGQHTVTLETGMMARQATAAVMVSMDDTAVFVTV
                     VGQKKAKPGQDFFPLTVNYQERTYAAGRIPGSFFRREGRPSEGETLIARLIDRPIRPL
                     FPEGFVNEVQVIATVVSVNPQVNPDIVAMIGASAALSLSGIPFNGPIGAARVGYINDQ
                     YVLNPTQDELKESKLDLVVAGTEAAVLMVESEAELLSEDQMLGAVVFGHEQQQVVIQN
                     INELVKEAGKPRWDWQPEPVNEALNARVAALAEARLSDAYRITDKQERYAQVDVIKSE
                     TIATLLAEDETLDENELGEILHAIEKNVVRSRVLAGEPRIDGREKDMIRGLDVRTGVL
                     PRTHGSALFTRGETQALVTATLGTARDAQVLDELMGERTDTFLFHYNFPPYSVGETGM
                     VGSPKRREIGHGRLAKRGVLAVMPDMDKFPYTVRVVSEITESNGSSSMASVCGASLAL
                     MDAGVPIKAAVAGIAMGLVKEGDNYVVLSDILGDEDHLGDMDFKVAGSRDGISALQMD
                     IKIEGITKEIMQVALNQAKGARLHILGVMEQAINAPRGDISEFAPRIHTIKINPDKIK
                     DVIGKGGSVIRALTEETGTTIEIEDDGTVKIAATDGEKAKNAIRRIEEITAEIEVGRV
                     YNGKVTRIVDFGAFVAIGGGKEGLVHISQIADKRVEKVTDYLQMGQEVPVKVLEVDRQ
                     GRIRLSIKEATEQSQPAAAPEAPAAEQGE"
     gene            complement(33296..33565)
                     /gene="rpsO"
                     /locus_tag="NBI74_000154"
     CDS             complement(33296..33565)
                     /gene="rpsO"
                     /locus_tag="NBI74_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HCD1930539.1"
                     /translation="MSLSTEATAKIVSEFGRDANDTGSTEVQVALLTAQINHLQGHFA
                     EHKKDHHSRRGLLRMVSQRRKLLDYLKRKDVARYTQLIERLGLRR"
     gene            complement(33714..34658)
                     /gene="truB"
                     /locus_tag="NBI74_000155"
     CDS             complement(33714..34658)
                     /gene="truB"
                     /locus_tag="NBI74_000155"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HCD1930540.1"
                     /translation="MSRPRRRGRDINGVLLLDKPQGMSSNDALQKVKRIYNANRAGHT
                     GALDPLATGMLPICLGEATKFSQYLLDSDKRYRVIARLGQRTDTSDADGQIVEERPVT
                     FSAEQLAAALDTFRGDIEQIPSMYSALKYQGKKLYEYARQGIEVPREARPITVYELLF
                     IRHEGNELELEIHCSKGTYIRTIIDDLGEKLGCGAHVIYLRRLAVSKYPVERMVTLEH
                     LRELVEQAEQQDIPAAELLDPLLMPMDSPASDYPVVNLPLTSSVYFKNGNPVRTSGAP
                     LEGLVRVTEGENGKFIGMGEIDDEGRVAPRRLVVEYPA"
     gene            complement(34658..35059)
                     /gene="rbfA"
                     /locus_tag="NBI74_000156"
     CDS             complement(34658..35059)
                     /gene="rbfA"
                     /locus_tag="NBI74_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001712908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HCD1930541.1"
                     /translation="MAKEFGRPQRVAQEMQKEIALILQREIKDPRLGMMTTVSGVEMS
                     RDLAYAKVYVTFLNDKDEDAVKAGIKALQEASGFIRSLLGKAMRLRIVPELTFFYDNS
                     LVEGMRMSNLVTSVVKHDEERRVNPDDSKED"
     gene            complement(35223..37895)
                     /gene="infB"
                     /locus_tag="NBI74_000157"
     CDS             complement(35223..37895)
                     /gene="infB"
                     /locus_tag="NBI74_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HCD1930542.1"
                     /translation="MTDVTIKTLAAERQTSVERLVQQFADAGIRKSADDSVSAQEKQT
                     LIDHLNQKNSGPDKLTLQRKTRSTLNIPGTGGKSKSVQIEVRKKRTFVKRDPQEAERL
                     AAEEQAQREAEEQARREAEESAKREAQQKAEREAAEQAKREAAEQAKREAAEKDKVSN
                     QQDDMTKNAQAEKARREQEAAELKRKAEEEARRKLEEEARRVAEEARRMAEENKWTDN
                     AEPTEDSSDYHVTTSQHARQAEDESDREVEGGRGRGRNAKAARPKKGNKHAESKADRE
                     EARAAVRGGKGGKRKGSSLQQGFQKPAQAVNRDVVIGETITVGELANKMAVKGSQVIK
                     AMMKLGAMATINQVIDQETAQLVAEEMGHKVILRRENELEEAVMSDRDTGAAAEPRAP
                     VVTIMGHVDHGKTSLLDYIRSTKVASGEAGGITQHIGAYHVETENGMITFLDTPGHAA
                     FTSMRARGAQATDIVVLVVAADDGVMPQTIEAIQHAKAAGVPVVVAVNKIDKPEADPD
                     RVKNELSQYGILPEEWGGESQFVHVSAKAGTGIDELLDAILLQAEVLELKAVRKGMAS
                     GAVIESFLDKGRGPVATVLVREGTLHKGDIVLCGFEYGRVRAMRNELGQEVLEAGPSI
                     PVEILGLSGVPAAGDEVTVVRDEKKAREVALYRQGKFREVKLARQQKSKLENMFANMT
                     EGEVHEVNIVLKADVQGSVEAISDSLLKLSTDEVKVKIIGSGVGGITETDATLAAASN
                     AILVGFNVRADASARKVIEAESLDLRYYSVIYNLIDEVKAAMSGMLSPELKQQIIGLA
                     EVRDVFKSPKFGAIAGCMVTEGVVKRHNPIRVLRDNVVIYEGELESLRRFKDDVNEVR
                     NGMECGIGVKNYNDVRTGDVIEVFEIIEIQRTIA"
     gene            complement(37920..39407)
                     /gene="nusA"
                     /locus_tag="NBI74_000158"
     CDS             complement(37920..39407)
                     /gene="nusA"
                     /locus_tag="NBI74_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005131833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HCD1930543.1"
                     /translation="MNKEILAVVEAVSNEKALPREKIFEALESALATATKKKYEQEID
                     VRVQIDRKSGDFDTFRRWLVVDEVTQPTKEITLEAARYEDESLNLGDYVEDQIESVTF
                     DRITTQTAKQVIVQKVREAERAMVVDQFREHEGEIITGVVKKVNRDNISLDLGNNAEA
                     VILREDMLPRENFRPGDRVRGVLYSVRPEARGAQLFVTRSKPEMLIELFRIEVPEIGE
                     EVIEIKAAARDPGSRAKIAVKTNDKRIDPVGACVGMRGARVQAVSTELGGERIDIVLW
                     DDNPAQFVINAMAPADVASIVVDEDKHTMDIAVEAGNLAQAIGRNGQNVRLASQLSGW
                     ELNVMTVDDLQAKHQAEAHAAIDTFTKYLDIDEDFATVLVEEGFSTLEELAYVPMKEL
                     LEIEGLDEPTVEALRERAKNALATIAQAQEESLGDNKPADDLLNLEGVDRDLAFKLAA
                     RGVCTLEDLAEQGIDDLADIEGLTDEKAGALIMAARNICWFGDEA"
     gene            complement(39435..39887)
                     /gene="rimP"
                     /locus_tag="NBI74_000159"
     CDS             complement(39435..39887)
                     /gene="rimP"
                     /locus_tag="NBI74_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HCD1930544.1"
                     /translation="MSTLEQKLTEMITAPVEALGFELVGIEFIRGRTSTLRIYIDSED
                     GINVDDCADVSHQVSAVLDVEDPITVAYNLEVSSPGLDRPLFTAEHYARFVGEEVTLV
                     LRMAVQNRRKWQGVIKAVDGEMITVTVEGKDEVFALSNIQKANLVPHF"
     gene            complement(40094..40170)
                     /locus_tag="NBI74_000160"
     tRNA            complement(40094..40170)
                     /locus_tag="NBI74_000160"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(40134..40136),aa:Met,seq:cat)
     gene            40518..41861
                     /gene="argG"
                     /locus_tag="NBI74_000161"
     CDS             40518..41861
                     /gene="argG"
                     /locus_tag="NBI74_000161"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HCD1930545.1"
                     /translation="MTTILKHLPVGQRIGIAFSGGLDTSAALLWMRQKGAVPYAYTAN
                     LGQPDEEDYDAIPRRAMEYGAENARLIDCRKQLVAEGIAAIQCGAFHNTTGGLTYFNT
                     TPLGRAVTGTMLVAAMKEDGVNIWGDGSTYKGNDIERFYRYGLLTNAELQIYKPWLDT
                     DFIDELGGRHEMSEFMIACGFDYKMSVEKAYSTDSNMLGATHEAKDLEYLNSSVKIVN
                     PIMGVKFWDESVKIPAEEVTVRFEQGHPVALNGKTFSDDVEMMLEANRIGGRHGLGMS
                     DQIENRIIEAKSRGIYEAPGMALLHIAYERLLTGIHNEDTIEQYHAHGRQLGRLLYQG
                     RWFDSQALMLRDSLQRWVASQITGEVTLELRRGNDYSILNTVSENLTYKPERLTMEKG
                     DSVFSPDDRIGQLTMRNLDITDTREKLFGYAKTGLLSSSAASGVPQVENLENKGQ"
     gene            complement(41869..43494)
                     /locus_tag="NBI74_000162"
     CDS             complement(41869..43494)
                     /locus_tag="NBI74_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417641.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HCD1930546.1"
                     /translation="MTVFNKFVKSFKSHWLLYLCVIIFGITNLVASSGAHMVQRLLFF
                     VLTILVVKRIPSLPLRLLIAAPFVLLTAADMSISLYSWCIFGTTFNDGFAISVLQSDP
                     DEVVKMLGMYIPYLCAFAFLSLLFLAVIIKYDVSLPTKKVTGILLLIVISGSLFSACQ
                     FAYKDAKNKEAFSPYILASRFATYTPFFNLNYFALAAKEHQRLLSIANTVPYFQLSVR
                     DTGIDTYVLIVGESVRVDNMSLYGYTHSTTPQVEAQRKQIKLFNQAISGAPYTALSVP
                     LSLTADSVLSHDIHNYPDNIINMANQAGFQTFWLSSQSAFRQNGTAVTSIAMRAMETV
                     YVRGFDELLLPHLSQALQQKTQQKKLIVLHLNGSHEPACSAYPQSSAVFQPQDDQDAC
                     YDNSIHYTDSLLGQVFELLKDRRASVMYFADHGLERDPTKKNVYFHGGREASQQAYHV
                     PMFIWYSPVLGDGVDRTTENNIFSTAYNNYIINAWMGVTKPEQPQTLEEVIAHYKGDS
                     RVVDANHDVFDYVMLRKEFTEDNQGNPTPEGQG"
     gene            complement(43951..44037)
                     /locus_tag="NBI74_000163"
     tRNA            complement(43951..44037)
                     /locus_tag="NBI74_000163"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(44001..44003),aa:Leu,seq:gag)
     gene            complement(44052..44384)
                     /gene="secG"
                     /locus_tag="NBI74_000164"
     CDS             complement(44052..44384)
                     /gene="secG"
                     /locus_tag="NBI74_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HCD1930547.1"
                     /translation="MYEALLVVFLIVAIGLVGLIMLQQGKGADMGASFGAGASATLFG
                     SSGSGNFMTRMTALLATLFFIISLVLGNINSNKTNKGSEWENLSAPAKTEQTQPAAPA
                     KPTSDIPN"
     gene            complement(44612..45949)
                     /gene="glmM"
                     /locus_tag="NBI74_000165"
     CDS             complement(44612..45949)
                     /gene="glmM"
                     /locus_tag="NBI74_000165"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HCD1930548.1"
                     /translation="MSNRKYFGTDGIRGRVGDAPITPDFVLKLGWAAGKVLARHGSRK
                     IIIGKDTRISGYMLESALEAGLAAAGLSALFTGPMPTPAVAYLTRTFRAEAGIVISAS
                     HNPFYDNGIKFFSIDGTKLPDAVEEAIEAEMEKEISCVDSAELGKASRIVDAAGRYIE
                     FCKATFPNELSLSELKIVVDCANGATYHIAPNVLRELGANVIAIGCEPNGVNINAEVG
                     ATDVRALQARVLAEKADLGIAFDGDGDRVIMVDHEGNKVDGDQIMYIIAREGLRQGQL
                     RGGAVGTLMSNMGLELALKQLGIPFARAKVGDRYVLEKMQEKGWRIGAENSGHVILLD
                     KTTTGDGIVAGLQVLAAMARNHMSLHDLCSGMKMFPQILVNVRYTAGSGDPLEHESVK
                     AVTAEVEAALGSRGRVLLRKSGTEPLIRVMVEGEDEAQVTEFAHRIADAVKAV"
     gene            complement(45942..46790)
                     /gene="folP"
                     /locus_tag="NBI74_000166"
     CDS             complement(45942..46790)
                     /gene="folP"
                     /locus_tag="NBI74_000166"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HCD1930549.1"
                     /translation="MKLFAQGTSLDLSHPHVMGILNVTPDSFSDGGTHNSLLDAVKHA
                     NLMINAGATIIDVGGESTRPGAAEVCVEEELQRVIPVVEAIAQRFEVWISVDTSKPEV
                     IRESAKVGAHIINDIRSLSEPGALEAAAETGLPVCLMHMQGNPKTMQEAPKYDDVFAE
                     VNRYFIEQIARCEQAGIAKEKLLLDPGFGFGKNLSHNYSLLARLAEFHHFNLPLLVGM
                     SRKSMIGQLLNVGPSERLSGSLACAVIAAMQGAHIIRVHDVKETVEAMRVVEATLSAK
                     ENKRYE"
     gene            complement(46880..48814)
                     /gene="ftsH"
                     /locus_tag="NBI74_000167"
     CDS             complement(46880..48814)
                     /gene="ftsH"
                     /locus_tag="NBI74_000167"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HCD1930550.1"
                     /translation="MAKNLILWLVIAVVLMSVFQSFGPSESNGRKVDYSTFLQEVNND
                     QVREARINGREINVTKKDSNRYTTYIPVQDPKLLDNLLTKNVKVVGEPPEEPSLLASI
                     FISWFPMLLLIGVWIFFMRQMQGGGGKGAMSFGKSKARMLTEDQIKTTFADVAGCDEA
                     KEEVAELVEYLREPSRFQKLGGKIPKGVLMVGPPGTGKTLLAKAIAGEAKVPFFTISG
                     SDFVEMFVGVGASRVRDMFEQAKKAAPCIIFIDEIDAVGRQRGAGLGGGHDEREQTLN
                     QMLVEMDGFEGNEGIIVIAATNRPDVLDPALLRPGRFDRQVVVGLPDVRGREQILKVH
                     MRRVPLAPDIDAAIIARGTPGFSGADLANLVNEAALFAARGNKRVVSMVEFEKAKDKI
                     MMGAERRSMVMTEAQKESTAYHEAGHAIIGRLVPEHDPVHKVTIIPRGRALGVTFFLP
                     EGDAISASRQKLESQISTLYGGRLAEEIIYGPEHVSTGASNDIKVATNLARNMVTQWG
                     FSEKLGPLLYAEEEGEVFLGRSVAKAKHMSDETARIIDQEVKALIERNYNRARQLLTD
                     NMDILHAMKDALMKYETIDAPQIDDLMARRDVRPPAGWEEPGASNNSGDNGSPKAPRP
                     VDEPRTPNPGNTMSEQLGDK"
     gene            complement(48914..49543)
                     /gene="rlmE"
                     /locus_tag="NBI74_000168"
     CDS             complement(48914..49543)
                     /gene="rlmE"
                     /locus_tag="NBI74_000168"
                     /EC_number="2.1.1.166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uridine(2552)-2'-O)-methyltransferase
                     RlmE"
                     /protein_id="HCD1930551.1"
                     /translation="MTGKKRSASSSRWLQEHFSDKYVQQAQKKGLRSRAWFKLDEIQQ
                     SDKLFKPGMTVVDLGAAPGGWSQYVVTQIGGKGRIIACDLLPMDPIVGVDFLQGDFRD
                     ELVMKALLERVGDSKVQVVMSDMAPNMSGTPAVDIPRAMYLVELALEMCRDVLAPGGS
                     FVVKVFQGEGFDEYLREIRSLFTKVKVRKPDSSRARSREVYIVATGRKP"
     gene            49669..49962
                     /gene="yhbY"
                     /locus_tag="NBI74_000169"
     CDS             49669..49962
                     /gene="yhbY"
                     /locus_tag="NBI74_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HCD1930552.1"
                     /translation="MNLSTKQKQHLKGLAHPLKPVVLLGSNGLTEGVLAEIEQALEHH
                     ELIKVKIATEDRETKTLIVEAIVRETGACNVQVIGKTLVLYRPTKERKISLPR"
     gene            complement(50118..50594)
                     /gene="greA"
                     /locus_tag="NBI74_000170"
     CDS             complement(50118..50594)
                     /gene="greA"
                     /locus_tag="NBI74_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006178768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HCD1930553.1"
                     /translation="MQAIPMTLRGAEKLREELDFLKSVRRPEIIAAIAEAREHGDLKE
                     NAEYHAAREQQGFCEGRIKDIEAKLSNAQVIDVTKMPNNGRVIFGATVTVLNLDSDEE
                     QTYRIVGDDEADFKQNLISVNSPIARGLIGKEEDDVVVIKTPGGEVEFEVIKVEYL"
     gene            50842..52275
                     /gene="dacB"
                     /locus_tag="NBI74_000171"
     CDS             50842..52275
                     /gene="dacB"
                     /locus_tag="NBI74_000171"
                     /EC_number="3.4.16.4"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001212660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HCD1930554.1"
                     /translation="MRFSRFIIGLTSCIAFSVQAANVDEYITQLPAGANLALMVQKVG
                     ASAPAIDYHSQQMALPASTQKVITALAALIQLGPDFRFTTTLETKGNVENGVLKGDLV
                     ARFGADPTLKRQDIRNMVTTLKKSGVNQIDGNVLIDTSIFASHDKAPGWPWNDMTQCF
                     SAPPAAAIVDRNCFSVSLYSAPKPGDMAFIRVASYYPVTMFSQVRTLPRGSAEAQYCE
                     LDVVPGDLNRFTLTGCLPQRSEPLPLAFAVQDGASYAGAILKDELKQAGIAWSGTLLR
                     QTQVNEPGTVVASKQSAPLHDLLKIMLKKSDNMIADTVFRMIGHARFNVPGTWRAGSD
                     AVRQILRQQAGVDIGNTIIADGSGLSRHNLIAPATMMQVLQYIAQHDNELNFISMLPL
                     AGYDGSLQYRAGLHQAGVDGKVSAKTGSLQGVYNLAGFITTASGQRMAFVQYLSGYAV
                     EPADQRNRRIPLVRFESRLYKDIYQNN"
     gene            complement(52316..53488)
                     /gene="cgtA"
                     /locus_tag="NBI74_000172"
     CDS             complement(52316..53488)
                     /gene="cgtA"
                     /locus_tag="NBI74_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Obg family GTPase CgtA"
                     /protein_id="HCD1930555.1"
                     /translation="MKFVDEASILVVAGDGGNGCVSFRREKYIPKGGPDGGDGGDGGD
                     VWMEADENLNTLIDYRFEKSFRAERGQNGASRDCTGKRGKDVTIKVPVGTRVIDQGTG
                     ETMGDMTKHGQRLLVAKGGWHGLGNTRFKSSVNRTPRQKTNGTPGDKRELLLELMLLA
                     DVGMLGMPNAGKSTFIRAVSAAKPKVADYPFTTLVPSLGVVRMDNEKSFVVADIPGLI
                     EGAAEGAGLGIRFLKHLERCRVLLHLIDIDPIDGTDPVENARIIISELEKYSQDLAAK
                     PRWLVFNKIDLLDKAEAEEKAKAIAEALGWEDKYYLISAASGLGVKDLCWDVMTFIIE
                     NPVVQAEEAKQPEKVEFMWDDYHRQQLEEIAEEDDEDWDDDWDEDDEEGVEFIYKR"
     gene            complement(53504..54469)
                     /locus_tag="NBI74_000173"
     CDS             complement(53504..54469)
                     /locus_tag="NBI74_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012135318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HCD1930556.1"
                     /translation="MKQQAGIGILLALTTAICWGALPIAMKQVLEVMEPPTIVFYRFL
                     MASIGLGAILAVKKRLPPLRVFRKPRWLILLAVATAGLFGNFILFSSSLQYLSPTASQ
                     VIGQLSPVGMMVASVFILKEKMRSTQVVGALMLLSGLVMFFNTSLVEIFTKLTDYTWG
                     VIFGVGAATVWVSYGVAQKVLLRRLASPQILFLLYTLCTIALFPLANPGVIAQLSHWQ
                     LACLIFCGLNTLVGYGALAEAMARWQAAQVSAIITLTPLFTLFFSDLLSLAWPDFFAR
                     PMLNLLGYLGAFVVVAGAMYSAIGHRIWGGLRKHTTVVSQPRAGE"
     gene            complement(54596..54853)
                     /gene="rpmA"
                     /locus_tag="NBI74_000174"
     CDS             complement(54596..54853)
                     /gene="rpmA"
                     /locus_tag="NBI74_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HCD1930557.1"
                     /translation="MAHKKAGGSTRNGRDSEAKRLGVKRFGGESVLAGSIIVRQRGTK
                     FHAGANVGCGRDHTLFAKADGKVKFEVKGPKNRKFISIEAE"
     gene            complement(54874..55185)
                     /gene="rplU"
                     /locus_tag="NBI74_000175"
     CDS             complement(54874..55185)
                     /gene="rplU"
                     /locus_tag="NBI74_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015960603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HCD1930558.1"
                     /translation="MYAVFQSGGKQHRVSEGQTVRLEKLDIATGETVEFAEVLMIANG
                     EEVKIGVPFVDGGVIKAEVVAHGRGEKVKIVKFRRRKHYRKQQGHRQWFTDVKITGIS
                     A"
     gene            55444..56415
                     /gene="ispB"
                     /locus_tag="NBI74_000176"
     CDS             55444..56415
                     /gene="ispB"
                     /locus_tag="NBI74_000176"
                     /EC_number="2.5.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="octaprenyl diphosphate synthase"
                     /protein_id="HCD1930559.1"
                     /translation="MNLEKINELTAQDMAGVNAAILEQLNSDVQLINQLGYYIVSGGG
                     KRIRPMIAVLAARAVGYEGNAHVTIAALIEFIHTATLLHDDVVDESDMRRGKATANAA
                     FGNAASVLVGDFIYTRAFQMMTSLGSLKVLEVMSEAVNVIAEGEVLQLMNVNDPDITE
                     ENYMRVIYSKTARLFEAAAQCSGILAGCTPEEEKGLQDYGRYLGTAFQLIDDLLDYNA
                     DGEQLGKNVGDDLNEGKPTLPLLHAMHHGTPEQAQMIRTAIEQGNGRHLLEPVLEAMN
                     ACGSLEWTRQRAEEEADKAIAALQVLPDTPWREALIGLAHIAVQRDR"
     gene            56648..56926
                     /gene="sfsB"
                     /locus_tag="NBI74_000177"
     CDS             56648..56926
                     /gene="sfsB"
                     /locus_tag="NBI74_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SfsB"
                     /protein_id="HCD1930560.1"
                     /translation="MESNFIDWHPADIIAGLRKKGTSMAAESRRNGLSSSTLANALSR
                     PWPKGEMIIAKALGTDPWVIWPSRYHDPQTHEFIDRTQLMRSYTKPKK"
     gene            complement(56974..58233)
                     /gene="murA"
                     /locus_tag="NBI74_000178"
     CDS             complement(56974..58233)
                     /gene="murA"
                     /locus_tag="NBI74_000178"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HCD1930561.1"
                     /translation="MDKFRVQGPTKLQGEVTISGAKNAALPILFAALLAEEPVEIQNV
                     PKLKDVDTSMKLLSQLGAKVERNGSVHIDARDVNVFCAPYDLVKTMRASIWALGPLVA
                     RFGQGQVSLPGGCTIGARPVDLHISGLEQLGATIKLEEGYVKASVDGRLKGAHIVMDK
                     VSVGATVTIMCAATLAEGTTIIENAAREPEIVDTANFLITLGAKISGQGTDRIVIEGV
                     ERLGGGVYRVLPDRIETGTFLVAAAISRGKIICRNAQPDTLDAVLAKLRDAGADIEVG
                     EDWISLDMHGKRPKAVNVRTAPHPAFPTDMQAQFTLLNLVAEGTGFITETVFENRFMH
                     VPELSRMGAHAEIESNTVICHGVEKLSGAQVMATDLRASASLVLAGCIAEGTTVVDRI
                     YHIDRGYERIEDKLRALGANIERVKGE"
     gene            complement(58288..58542)
                     /gene="ibaG"
                     /locus_tag="NBI74_000179"
     CDS             complement(58288..58542)
                     /gene="ibaG"
                     /locus_tag="NBI74_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family iron metabolism protein IbaG"
                     /protein_id="HCD1930562.1"
                     /translation="MENNEIQSVLMNALSLQEVHVSGDGSHFQVIAVGELFDGMSRVK
                     KQQTVYGPLMEYIADNRIHAVSIKAYTPAEWARDRKLNGF"
     gene            complement(58702..58995)
                     /gene="mlaB"
                     /locus_tag="NBI74_000180"
     CDS             complement(58702..58995)
                     /gene="mlaB"
                     /locus_tag="NBI74_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance protein MlaB"
                     /protein_id="HCD1930563.1"
                     /translation="MSESLSWMQTGDTLALSGELDQDVLLPLWEIREEAVKGITSIDL
                     SRVSRVDTGGLALLLHLIDLAKKQGNNVTLQGVNDKVYTLAKLYNLPADVLPR"
     gene            complement(58995..59630)
                     /gene="mlaC"
                     /locus_tag="NBI74_000181"
     CDS             complement(58995..59630)
                     /gene="mlaC"
                     /locus_tag="NBI74_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding protein MlaC"
                     /protein_id="HCD1930564.1"
                     /translation="MFKRLMMVALLVIAPLSAATAADQTNPYKLMDEAAQKTFDRLKN
                     EQPQIRANPDYLRTIVDQELLPYVQVKYAGALVLGQYYKSATPAQRDAYFAAFREYLK
                     QAYGQALAMYHGQTYQIAPEQPLGDKTIVPIRVTIIDPNGRPPVRLDFQWRKNSQTGN
                     WQAYDMIAEGVSMITTKQNEWGTLLRTKGIDGLTAQLKSISQQKITLEEKK"
     gene            complement(59649..60200)
                     /gene="mlaD"
                     /locus_tag="NBI74_000182"
     CDS             complement(59649..60200)
                     /gene="mlaD"
                     /locus_tag="NBI74_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HCD1930565.1"
                     /translation="MQTKKNEIWVGIFLLAALLAALFVCLKAANVTSIRTEPTYTLYA
                     TFDNIGGLKARSPVSIGGVVVGRVADITLDPKTYLPRVTLEIEQRYNHIPDTSSLSIR
                     TSGLLGEQYLALNVGFEDPELGTAILKDGDTIQDTKSAMVLEDLIGQFLYGSKGDDNK
                     NSGDAPAAAPGNNETTEPVGTTK"
     gene            complement(60205..60987)
                     /gene="mlaE"
                     /locus_tag="NBI74_000183"
     CDS             complement(60205..60987)
                     /gene="mlaE"
                     /locus_tag="NBI74_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014071945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance ABC transporter
                     permease subunit MlaE"
                     /protein_id="HCD1930566.1"
                     /translation="MLLNALASLGHKGIKTLRTFGRAGLMLFNALVGKPEFRKHAPLL
                     VRQLYNVGVLSMLIIVVSGVFIGMVLGLQGYLVLTTYSAETSLGMLVALSLLRELGPV
                     VAALLFAGRAGSALTAEIGLMRATEQLSSMEMMAVDPLRRVISPRFWAGVISLPLLTV
                     IFVAVGIWGGSLVGVSWKGIDSGFFWSAMQNAVDWRMDLVNCLIKSVVFAITVTWISL
                     FNGYDAIPTSAGISRATTRTVVHSSLAVLGLDFVLTALMFGN"
     gene            complement(60995..61804)
                     /gene="mlaF"
                     /locus_tag="NBI74_000184"
     CDS             complement(60995..61804)
                     /gene="mlaF"
                     /locus_tag="NBI74_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid ABC transporter ATP-binding protein
                     MlaF"
                     /protein_id="HCD1930567.1"
                     /translation="MEQSVANLVDMRDVSFTRGNRCIFDNISLTVPRGKITAIMGPSG
                     IGKTTLLRLIGGQIAPDHGEILFDGENIPAMSRSRLYTVRKRMSMLFQSGALFTDMNV
                     FDNVAYPLREHTQLPAPLLHSTVMMKLEAVGLRGAAKLMPSELSGGMARRAALARAIA
                     LEPDLIMFDEPFVGQDPITMGVLVKLISELNSALGVTCVVVSHDVPEVLSIADHAWIL
                     ADKKIVAHGSAQALQANPDPRVRQFLDGIADGPVPFRYPAGDYHADLLPGS"
     gene            62014..62991
                     /locus_tag="NBI74_000185"
     CDS             62014..62991
                     /locus_tag="NBI74_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HCD1930568.1"
                     /translation="MLLATALLIVGLLLVVYSADRLVFAASILCRTFGIPPLIIGMTV
                     VSIGTSLPEIIVSLAASLHEQRDLAVGTALGSNIINILLILGLAALVRPFTVHSDVLR
                     RELPLMLLVSVVAGSVLYDGQLSRSDGIFLLFLAVLWLLFIVKLARQAERQGTDSLTR
                     EQLAELPREGGLPVAFLWLGIALIIMPVATRMVVDNATVLANYFAISELTMGLTAIAI
                     GTSLPELATAIAGVRKGENDIAVGNIIGANIFNIVIVLGLPALITPGEIDPLAYSRDY
                     SVMLLVSIIFALLCWRRSPQPGRGVGVLLTGGFIVWLAMLYWLSPILVE"
     gene            63005..63991
                     /gene="kdsD"
                     /locus_tag="NBI74_000186"
     CDS             63005..63991
                     /gene="kdsD"
                     /locus_tag="NBI74_000186"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase KdsD"
                     /protein_id="HCD1930569.1"
                     /translation="MSHVELQPGFDFQQAGKEVLAIERECLAELDQYINQNFTLACEK
                     MFWCKGKVVVMGMGKSGHIGRKMAATFASTGTPSFFVHPGEAAHGDLGMVTPQDVVIA
                     ISNSGESSEITALIPVLKRLHVPLICITGRPESSMARAADVHLCVKVAKEACPLGLAP
                     TSSTTATLVMGDALAVALLKARGFTAEDFALSHPGGALGRKLLLRVNDIMHTGDEIPH
                     VKKTASLRDALLEVTRKNLGMTVICDDNMMIEGIFTDGDLRRVFDMGVDVRQLSIADV
                     MTPGGIRVRPGILAVEALNLMQSRHITSVMVADGDHLLGVLHMHDLLRAGVV"
     gene            64012..64578
                     /gene="kdsC"
                     /locus_tag="NBI74_000187"
     CDS             64012..64578
                     /gene="kdsC"
                     /locus_tag="NBI74_000187"
                     /EC_number="3.1.3.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate-8-phosphatase KdsC"
                     /protein_id="HCD1930570.1"
                     /translation="MSKAGASLATCYGPVSADVMAKAENIRLLILDVDGVLSDGLIYM
                     GNNGEELKAFNVRDGYGIRCALTSDIEVAIITGRKAKLVEDRCATLGITHLYQGQSNK
                     LIAFSDLLEKLAIAPENVAYVGDDLIDWPVMEKVGLSVAVADAHPLLIPRADYVTRIA
                     GGRGAVREVCDLLLLAQGKLDEAKGQSI"
     gene            64575..65150
                     /gene="lptC"
                     /locus_tag="NBI74_000188"
     CDS             64575..65150
                     /gene="lptC"
                     /locus_tag="NBI74_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HCD1930571.1"
                     /translation="MSKARRWVIIVLSLAVLVMIGINMAEKDDTAQVVVNNNDPTYKS
                     EHTDTLVYNPEGALSYRLIAQHVEYYSDQAVSWFTQPVLTTFDKDKIPTWSVKADKAK
                     LTNDRMLYLYGHVEVNALVPDSQLRRITTDNAQINLVTQDVTSEDLVTLYGTTFNSSG
                     LKMRGNLRSKNAELIEKVRTSYEIQNKQTQP"
     gene            65119..65676
                     /gene="lptA"
                     /locus_tag="NBI74_000189"
     CDS             65119..65676
                     /gene="lptA"
                     /locus_tag="NBI74_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide ABC transporter
                     substrate-binding protein LptA"
                     /protein_id="HCD1930572.1"
                     /translation="MKFKTNKLSLNLVLASSLLAASIPAFAVTGDTDQPIHIESDQQS
                     LDMQGNVVTFTGNVIVTQGTIKINADKVVVTRPGGEQGKEVIDGYGKPATFYQMQDNG
                     KPVEGHASQMHYELAKDFVVLTGNAYLQQVDSNIKGDKITYLVKEQKMQAFSDKGKRV
                     TTVLVPSQLQDKNNKGQTPAQKKGN"
     gene            65683..66408
                     /gene="lptB"
                     /locus_tag="NBI74_000190"
     CDS             65683..66408
                     /gene="lptB"
                     /locus_tag="NBI74_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HCD1930573.1"
                     /translation="MATLTAKNLAKAYKGRRVVEDVSLTVNSGEIVGLLGPNGAGKTT
                     TFYMVVGIVPRDAGNIIIDDDDISLLPLHARARRGIGYLPQEASIFRRLSVYDNLMAV
                     LQIRDDLSAEQREDRANELMEEFHIEHLRDSMGQSLSGGERRRVEIARALAANPKFIL
                     LDEPFAGVDPISVIDIKRIIEHLRDSGLGVLITDHNVRETLAVCERAYIVSQGHLIAH
                     GTPTEILQDEHVKRVYLGEDFRL"
     gene            66456..67889
                     /gene="rpoN"
                     /locus_tag="NBI74_000191"
     CDS             66456..67889
                     /gene="rpoN"
                     /locus_tag="NBI74_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HCD1930574.1"
                     /translation="MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALESN
                     PLLEQIDTHEEIDTRETQDSETLDTADALEQKEMPEELPLDASWDTIYTAGTPSGTSG
                     DYIDDELPVYQGETTQTLQDYLMWQVELTPFSDTDRAIATSIVDAVDDTGYLTVPLED
                     ILESMGDEEIDIDEVEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFDKTTPWLEEARL
                     IISDHLDLLANHDFRTLMRVTRLKEDVLKEAVNLIQSLDPRPGQSIQTGEPEYVIPDV
                     LVRKHNGHWTVELNSDSIPRLQINQHYASMCNNARNDGDSQFIRSNLQDAKWLIKSLE
                     SRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHS
                     PRGIFELKYFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTSLLSEQG
                     IMVARRTVAKYRESLSIPPSNQRKQLV"
     gene            67912..68199
                     /gene="hpf"
                     /locus_tag="NBI74_000192"
     CDS             67912..68199
                     /gene="hpf"
                     /locus_tag="NBI74_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome hibernation promoting factor"
                     /protein_id="HCD1930575.1"
                     /translation="MQLNITGNNVEITEALREFVTAKFAKLEQYFDRINQVYVVLKVE
                     KVTHTSDATLHVNGGEIHASAEGQDMYAAIDGLIDKLARQLTKHKDKLKQH"
     gene            68317..68808
                     /gene="ptsN"
                     /locus_tag="NBI74_000193"
     CDS             68317..68808
                     /gene="ptsN"
                     /locus_tag="NBI74_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS IIA-like nitrogen regulatory protein PtsN"
                     /protein_id="HCD1930576.1"
                     /translation="MTNNDTTLQLSSVLNRECTRSRVHCQSKKRALEIISELAAKQLS
                     LAPQVVFEAILTREKMGSTGIGNGIAIPHGKLEEDTLRAVGVFVQLETPIAFDAIDNQ
                     PVDLLFALLVPADQTKTHLHTLSLVAKRLADKTICRRLRAAQSDEELYQIITDTEGTP
                     DEA"
     gene            68854..69708
                     /gene="rapZ"
                     /locus_tag="NBI74_000194"
     CDS             68854..69708
                     /gene="rapZ"
                     /locus_tag="NBI74_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HCD1930577.1"
                     /translation="MVLMIVSGRSGSGKSVALRALEDMGFYCVDNLPVVLLPDLARTL
                     ADREISAAVSIDVRNMPESPEIFEQAMSNLPDAFSPQLLFLDADRNTLIRRYSDTRRL
                     HPLSSKNLSLESAIDKESDLLEPLRSRADLIVDTSEMSVHELAEMLRTRLLGKREREL
                     TMVFESFGFKHGIPIDADYVFDVRFLPNPHWDPKLRPMTGLDKPVAAFLDRHTEVHNF
                     IYQTRSYLELWLPMLETNNRSYLTVAIGCTGGKHRSVYIAEQLADYFRSRGKNVQSRH
                     RTLEKRKP"
     gene            69705..69977
                     /gene="npr"
                     /locus_tag="NBI74_000195"
     CDS             69705..69977
                     /gene="npr"
                     /locus_tag="NBI74_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS phosphocarrier protein NPr"
                     /protein_id="HCD1930578.1"
                     /translation="MTVKQTVEITNKLGMHARPAMKLFELMQGFDAEVLLRNDEGTEA
                     EANSVIALLMLDSAKGRQIEVEATGPQEEEALAAVIALFNSGFDED"
     gene            70190..70822
                     /gene="yrbL"
                     /locus_tag="NBI74_000196"
     CDS             70190..70822
                     /gene="yrbL"
                     /locus_tag="NBI74_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP regulatory network protein YrbL"
                     /protein_id="HCD1930579.1"
                     /translation="MIRLSEQSPLGTGRHRKCYAHPEDAQRCIKIVYHRGDGGDKEIR
                     RELKYYAHLGRRLKDWSGIPRYHGTVETDCGTGYVYDVIADFDGKPSITLTEFAEQCR
                     YEEDIAQLRQLLKQLKRYLQDNRIVTMSLKPQNILCHRISESEVIPVVCDNIGESTLI
                     PLATWSKWCCLRKQERLWKRFIAQPALAIALQKDLQPRESKTLALTSREA"
     gene            complement(70819..71547)
                     /gene="mtgA"
                     /locus_tag="NBI74_000197"
     CDS             complement(70819..71547)
                     /gene="mtgA"
                     /locus_tag="NBI74_000197"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HCD1930580.1"
                     /translation="MSKSRLTVFSFVRRFLLRLMVVLAIFWGGGIALFSVAPVPFSAV
                     MVERQVSAWLHGNFRYVAHSDWVSMDQISPWMGLAVIAAEDQKFPEHWGFDVASIEQA
                     LAHNERNENRIRGASTISQQTAKNLFLWDGRSWVRKGLEAGLTLGIETVWSKKRILTV
                     YLNIAEFGDGVFGVEAAAQRYFHKPASKLTRSEAALLAAVLPNPLRFKVSAPSGYVRS
                     RQAWILRQMYQLGGEPFMQQHQLD"
     gene            complement(71544..72197)
                     /gene="elbB"
                     /locus_tag="NBI74_000198"
     CDS             complement(71544..72197)
                     /gene="elbB"
                     /locus_tag="NBI74_000198"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenoid biosynthesis glyoxalase ElbB"
                     /protein_id="HCD1930581.1"
                     /translation="MKKIGVILSGCGVYDGSEIHEAVLTLLAISRSGAQAVCFAPDKQ
                     QVDVINHLTGEAMTETRNVLIEAARITRGEIRPLAQADAAELDALIVPGGFGAAKNLS
                     NFASLGSECTVDRELKELAQAMHQAGKPLGFMCIAPAILPKIFDFPLRLTIGTDIDTA
                     EVLEEMGAEHVPCPVDDIVVDEDNKIVTTPAYMLAQNIAEAASGIDKLVSRVLVLAE"
     gene            complement(72427..74763)
                     /gene="arcB"
                     /locus_tag="NBI74_000199"
     CDS             complement(72427..74763)
                     /gene="arcB"
                     /locus_tag="NBI74_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobic respiration two-component sensor
                     histidine kinase ArcB"
                     /protein_id="HCD1930582.1"
                     /translation="MKQIRLLAQYYVDLMMKLGLVRFSMLLALALVVLAIVVQMAVTM
                     VLHGQVESIDVIRSIFFGLLITPWAVYFLSVVVEQLEESRQRLSRLVQKLEEMRERDL
                     SLNVQLKDNIAQLNQEIAVREKAEAELQETFGQLKIEIKEREETQIQLEQQSSFLRSF
                     LDASPDLVFYRNEDKEFSGCNRAMELLTGKSEKQLVHLKPADVYSPEAAAKVIETDEK
                     VFRHNVSLTYEQWLDYPDGRKACFEIRKVPYYDRVGKRHGLMGFGRDITERKRYQDAL
                     ERASRDKTTFISTISHELRTPLNGIVGLSRILLDTELTAEQEKYLKTIHVSAVTLGNI
                     FNDIIDMDKMERRKVQLDNQPVDFTSFLADLENLSALQAQQKGLRFNLEPTLPLPHQV
                     ITDGTRLRQILWNLISNAVKFTQQGQVTVRVRYDEGDMLHFEVEDSGIGIPQDELDKI
                     FAMYYQVKDSHGGKPATGTGIGLAVSRRLAKNMGGDITVTSEQGKGSTFTLTIHAPSV
                     AEEVDDAFDEDDMPLPALNVLLVEDIELNVIVARSVLEKLGNSVDVAMTGKAALEMFK
                     PGEYDLVLLDIQLPDMTGLDISRELTKRYPREDLPPLVALTANVLKDKQEYLNAGMDD
                     VLSKPLSVPALTAMIKKFWDTQDDEESTVTTEENSKSEALLDIPMLEQYLELVGPKLI
                     TDGLAVFERMMPGYVSVLESNLTAQDKKGIVEEGHKIKGAAGSVGLRHLQQLGQQIQS
                     PDLPAWEDNVGEWIEEMKEEWRHDVEVLKAWVAKATKK"
     gene            complement(74859..75788)
                     /locus_tag="NBI74_000200"
     CDS             complement(74859..75788)
                     /locus_tag="NBI74_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562163.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HCD1930583.1"
                     /translation="MQLQKLVNMFGGDLTRRYGQKVHKLTLHGGFSCPNRDGTIGRGG
                     CTFCNVASFADEAQQHRSIAEQLAHQANLVNRAKRYLAYFQAYTSTFAEVQVLRSMYQ
                     QAVSQANIVGLCVGTRPDCVPDAVLDLLCEYKDQGYEVWLELGLQTAHDKTLHRINRG
                     HDFACYQRTTQLARERGLKVCSHLIVGLPGEGQAECLQTLERVVETGVDGIKLHPLHI
                     VKGSIMAKAWEAGRLNGIELEDYTLTAGEMIRHTPPEVIYHRISASARRPTLLAPLWC
                     ENRWTGMVELDRYLNEHGVQGSALGRPWLPPTA"
     gene            76463..80923
                     /gene="gltB"
                     /locus_tag="NBI74_000201"
     CDS             76463..80923
                     /gene="gltB"
                     /locus_tag="NBI74_000201"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HCD1930584.1"
                     /translation="MLYDKSLERDNCGFGLIAHIEGEPSHKVVRTAIHALARMQHRGA
                     ILADGKTGDGCGLLLQKPDRFFRIVAQERGWRLAKNYAVGMLFLNKDPELAAAARRIV
                     EEELQRETLSIVGWRDVPTNEGVLGEIALSSLPRIEQIFVNAPAGWRPRDMERRLFIA
                     RRRIEKRLEADKDFYVCSLSNLVNIYKGLCMPADLPRFYLDLADLRLESAICLFHQRF
                     STNTVPRWPLAQPFRYLAHNGEINTITGNRQWARARTYKFQTPLIPDLHDAAPFVNET
                     GSDSSSMDNMLELLLAGGMDIIRAMRLLVPPAWQNNPDMDPELRAFFDFNSMHMEPWD
                     GPAGIVMSDGRFAACNLDRNGLRPARYVITKDKLITCASEVGIWDYQPDEVVEKGRVG
                     PGELMVIDTRSGRILHSAETDDDLKSRHPYKEWMEKNVRRLVPFEDLPDEEVGSRELD
                     DDTLASYQKQFNYSAEELDSVIRVLGENGQEAVGSMGDDTPFAVLSSQPRIIYDYFRQ
                     QFAQVTNPPIDPLREAHVMSLATSIGREMNVFCEAEGQAHRLSFKSPILLYSDFKQLT
                     TMKEDHYRADTLDITFDVTKTTLEATVKELCDKAEKMVRSGTVLLVLSDRNIAKDRLP
                     VPAPMAVGAIQTRLVEQSLRCDANIIVETASARDPHHFAVLLGFGATAIYPYLAYETL
                     GRLVDTHAIAKDYRTVMLNYRNGINKGLYKIMSKMGISTIASYRCSKLFEAVGLHDDV
                     VGLCFQGAVSRIGGASFEDFQQDLLNLSKRAWLARKPISQGGLLKYVHGGEYHAYNPD
                     VVRTLQQAVQSGEYSDYQEYAKLVNERPATTLRDLLAITPGENAVNIADVEPASELFK
                     RFDTAAMSIGALSPEAHEALAEAMNSIGGNSNSGEGGEDPARYGTNKVSRIKQVASGR
                     FGVTPAYLVNADVIQIKVAQGAKPGEGGQLPGDKVTPYIAKLRYSVPGVTLISPPPHH
                     DIYSIEDLAQLIFDLKQVNPKAMISVKLVSEPGVGTIATGVAKAYADLITIAGYDGGT
                     GASPLSSVKYAGCPWELGLVETQQALVANGLRHKIRLQVDGGLKTGVDIIKAAILGAE
                     SFGFGTGPMVALGCKYLRICHLNNCATGVATQDDKLRKNHYHGLPFKVTNYFEFIARE
                     TRELMAQLGVTRLVDLIGRTDLLKELDGFTAKQQKLALSKLLETAEPHPGKALYCTEN
                     NPPFDNGLLNAQLLQQAKPFVDERQSKTFWFDIRNTDRSVGASLSGYIAQTHGDQGLA
                     ADPIKAYFNGTAGQSFGVWNAGGVELYLTGDANDYIGKGMAGGLIAIRPPVGSAFRSH
                     EASIIGNTCLYGATGGRLYAAGRAGERFGVRNSGAITVVEGIGDNGCEYMTGGIVCIL
                     GKTGVNFGAGMTGGFAYVLDESGDFRKRVNPELVEVLSVDDLAIHEEHLRGLITEHVQ
                     HTGSQRGEEILANWSTFATKFALVKPKSSDVKALLGHRSRSAAELRVQAQ"
     gene            80936..82354
                     /gene="gltD"
                     /locus_tag="NBI74_000202"
     CDS             80936..82354
                     /gene="gltD"
                     /locus_tag="NBI74_000202"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit GltD"
                     /protein_id="HCD1930585.1"
                     /translation="MSQNVYQFIDLQRVDPPKKPLKIRKIEFVEIYEPFSEGQAKAQA
                     DRCLSCGNPYCEWKCPVHNYIPNWLKLANEGRIFEAAELSHQTNTLPEVCGRVCPQDR
                     LCEGSCTLNDEFGAVTIGNIERYINDKAFEMGWRPDMSGVKQTGKKVAIIGAGPAGLA
                     CADVLTRNGVKAIVFDRHPEIGGLLTFGIPAFKLEKEVMTRRREIFTGMGIEFKLNTE
                     VGRDVQLDDLLSDYDAVFLGVGTYQSMRGGLENEDADGVYAALPFLIANTKQLMGFGE
                     TRDEPFVSMEGKRVVVLGGGDTAMDCVRTSVRQGAKHVTCAYRRDEENMPGSRREVKN
                     AREEGVEFKFNVQPLGIEVNGNGKVSGVKMVRTEMGEPDAKGRRRAEIVAGSEHIVPA
                     DAVIMAFGFRPHNMEWLAKHSVELDSQGRIIAPEGSDNAFQTSNPKIFAGGDIVRGSD
                     LVVTAIAEGRKAADGIMNWLEV"
     gene            complement(82422..82886)
                     /locus_tag="NBI74_000203"
     CDS             complement(82422..82886)
                     /locus_tag="NBI74_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HCD1930586.1"
                     /translation="MMMGEVQSLPSAGLHPALQDALTLALAARPQEKAPGRYELQGDN
                     IFMNVMTFNTQSPVEKKAELHEQYIDIQLLLNGEERILFGMAGTARQCEEFHHEDDYQ
                     LCSAIENEQAIILKPGMFAVFMPGEPHKPGCVVGEPGEIKKVVVKVKADLMA"
     gene            complement(82883..83758)
                     /gene="nanK"
                     /locus_tag="NBI74_000204"
     CDS             complement(82883..83758)
                     /gene="nanK"
                     /locus_tag="NBI74_000204"
                     /EC_number="2.7.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine kinase"
                     /protein_id="HCD1930587.1"
                     /translation="MTTLAIDIGGTKLAAALIGADGQIRDRRELPTPASQTPQALRDA
                     LSALVSPLQAHAQRVAIASTGIIRDGSLLALNPHNLGGLLHFPLVKTLEQLTNLPTIA
                     INDAQAAAWAEYQALDGDITDMVFITVSTGVGGGVVSGGKLRTGPGGLAGHIGHTLAD
                     PHGPVCGCGRTGCVEAIASGRGIAAAAQGELAGANAKTIFTRAGQGDEQAQQLIHRSA
                     RTLARLIADIKATTDCQCVVVGGSVGLAEGYLALVETYLAQEPAAFHVDLLAAHYRHD
                     AGLLGAALLAQGEIL"
     gene            complement(83755..84444)
                     /locus_tag="NBI74_000205"
     CDS             complement(83755..84444)
                     /locus_tag="NBI74_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HCD1930588.1"
                     /translation="MSLLAQLDQKIAANGGLIVSCQPVPDSPLDKPEIVAAMALAAEQ
                     AGAVAIRIEGVANLQATRAVVSVPIIGIVKRDLEDSPVRITAYIEDVDALAQAGADII
                     AIDGTDRPRPVPVETLLARIHHHGLLAMTDCSTPEDGLACQKLGAEIIGTTLSGYTTP
                     ETPEEPDLALVKTLSDAGCRVIAEGRYNTPAQAADAMRHGAWAVTVGSAITRLEHICQ
                     WYNTAMKKAVL"
     gene            complement(84492..85982)
                     /gene="nanT"
                     /locus_tag="NBI74_000206"
     CDS             complement(84492..85982)
                     /gene="nanT"
                     /locus_tag="NBI74_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialic acid transporter NanT"
                     /protein_id="HCD1930589.1"
                     /translation="MSTTTQNIPWYRHLNRAQWRAFSAAWLGYLLDGFDFVLIALVLT
                     EVQGEFGLTTVQAASLISAAFISRWFGGLMLGAMGDRYGRRLAMVTSIVLFSAGTLAC
                     GFAPGYITMFIARLVIGMGMAGEYGSSATYVIESWPKHLRNKASGFLISGFSVGAVVA
                     AQVYSLVVPVWGWRALFFIGILPIIFALWLRKNIPEAEDWKEKHGGKAPVRTMVDILY
                     RGEHRIANIVMTLAAATALWFCFAGNLQNAAIVAVLGLLCAAIFISFMVQSTGKRWPT
                     GVMLMVVVLFAFLYSWPIQALLPTYLKTDLAYDPHTVANVLFFSGFGAAVGCCVGGFL
                     GDWLGTRKAYVCSLLASQLLIIPVFAIGGANVWVLGLLLFFQQMLGQGISGILPKLIG
                     GYFDTDQRAAGLGFTYNVGALGGALAPILGALIAQRLDLGTALGSLSFSLTFVVILLI
                     GLDMPSRVQRWLRPEALRTHDAIDGKPFSGAVPFGSAKNDLVKTKS"
     gene            complement(86091..86984)
                     /gene="nanA"
                     /locus_tag="NBI74_000207"
     CDS             complement(86091..86984)
                     /gene="nanA"
                     /locus_tag="NBI74_000207"
                     /EC_number="4.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HCD1930590.1"
                     /translation="MATNLRGVMAALLTPFDQQQALDKASLRRLVQFNIQQGIDGLYV
                     GGSTGEAFVQSLSEREQVLEIVAEEAKGKIKLIAHVGCVSTAESQQLAASAKRYGFDA
                     VSAVTPFYYPFSFEEHCDHYRAIIDSADGLPMVVYNIPALSGVKLTLDQINTLVTLPG
                     VGALKQTSGDLYQMEQIRREHPDLVLYNGYDEIFASGLLAGADGGIGSTYNIMGWRYQ
                     GIVKALKEGDIQTAQKLQTECNKVIDLLIKTGVFRGLKTVLHYMDVVSVPLCRKPFGP
                     VDEKYLPELKALAQQLMQERG"
     gene            complement(87106..87897)
                     /gene="nanR"
                     /locus_tag="NBI74_000208"
     CDS             complement(87106..87897)
                     /gene="nanR"
                     /locus_tag="NBI74_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417693.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NanR"
                     /protein_id="HCD1930591.1"
                     /translation="MSPMNAFDPQAEDSTTTIGRNLRSRPLARKKLSEMVEEELEQMI
                     RRREFGEGEQLPSERELMAFFNVGRPSVREALAALKRKGLVQINNGERARVSRPSADT
                     IIGELSGMAKDFLSHPGGIAHFEQLRLFFESSLVRYAAEHATDEQIDLLAKALEINSQ
                     SLDNNAAFIRSDVDFHRVLAEIPGNPIFMAIHVALLDWLIAARPTVTDQALHEHNNVS
                     YQQHIAIVDAIRRHDPDEADRALQSHLNSVSATWHAFGQTTNKKK"
     gene            complement(88005..88502)
                     /gene="sspB"
                     /locus_tag="NBI74_000209"
     CDS             complement(88005..88502)
                     /gene="sspB"
                     /locus_tag="NBI74_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor"
                     /protein_id="HCD1930592.1"
                     /translation="MDLSQLTPRRPYLLRAFYEWLLDNQLTPHLVVDVTLPGVQVPME
                     YARDGQIVLNIAPRAVGNLELANDEVRFNARFGGIPRQVSVPLAAVLAIYARENGAGT
                     MFEPEAAYDEDASIMNDEEASADNETVMSVIDGDKPDHDDETHPDDEPPQPPRGGRPA
                     LRVVK"
     gene            complement(88508..89146)
                     /gene="sspA"
                     /locus_tag="NBI74_000210"
     CDS             complement(88508..89146)
                     /gene="sspA"
                     /locus_tag="NBI74_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stringent starvation protein A"
                     /protein_id="HCD1930593.1"
                     /translation="MAVAANKRSVMTLFSGPTDIYSHQVRIVLAEKGVSFEIEHVEKD
                     NPPQDLIDLNPNQSVPTLVDRELTLWESRIIMEYLDERFPHPPLMPVYPVARGESRLY
                     MHRIEKDWYTLMNTIINGSASEADAARKQLREELLAIAPVFGQKPYFLSDEFSLVDCY
                     LAPLLWRLPQLGIEFSGPGAKELKGYMTRVFERDSFLASLTEAEREMRLGRS"
     gene            complement(89541..89933)
                     /gene="rpsI"
                     /locus_tag="NBI74_000211"
     CDS             complement(89541..89933)
                     /gene="rpsI"
                     /locus_tag="NBI74_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HCD1930594.1"
                     /translation="MAENQYYGTGRRKSSAARVFIKPGNGKIVINQRSLEQYFGRETA
                     RMVVRQPLELVDMVEKLDLYITVKGGGISGQAGAIRHGITRALMEYDESLRSELRKAG
                     FVTRDARQVERKKVGLRKARRRPQFSKR"
     gene            complement(89949..90377)
                     /gene="rplM"
                     /locus_tag="NBI74_000212"
     CDS             complement(89949..90377)
                     /gene="rplM"
                     /locus_tag="NBI74_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HCD1930595.1"
                     /translation="MKTFTAKPETVKRDWYVVDATGKTLGRLATELARRLRGKHKAEY
                     TPHVDTGDYIIVLNADKVAVTGNKRTDKVYYHHTGHIGGIKQATFEEMIARRPERVIE
                     IAVKGMLPKGPLGRAMFRKLKVYAGNEHNHAAQQPQVLDI"
     gene            complement(90597..91724)
                     /gene="zapE"
                     /locus_tag="NBI74_000213"
     CDS             complement(90597..91724)
                     /gene="zapE"
                     /locus_tag="NBI74_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapE"
                     /protein_id="HCD1930596.1"
                     /translation="MQSVTPTSQYLKALNEGSHQPDDVQKEAVSRLEIIYKELVNSTP
                     PAPRTSGLMARVGKLWGKREDTKHMPVRGLYMWGGVGRGKTWLMDLFYQSLPGERKQR
                     LHFHRFMLRVHEELTALQGQTDPLEIIADRFKAETDVLCFDEFFVSDITDAMLLGGLM
                     KALFARGITLVATSNIPPDELYRNGLQRARFLPAIDAIKQHCDVMNVDAGVDYRLRTL
                     TQAHLWLSPLNDETRAQMDKLWLALAGAKRENSPTLEINHRPLATMGVENQTLAVSFT
                     TLCVDARSQHDYIALSRLFHTVMLFDVPVMTRLMESEARRFIALVDEFYERHVKLVVS
                     AEVPLYEIYQGERLKFEFQRCLSRLQEMQSEEYLKREHLAG"
     gene            91918..92316
                     /locus_tag="NBI74_000214"
     CDS             91918..92316
                     /locus_tag="NBI74_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1043 family protein"
                     /protein_id="HCD1930597.1"
                     /translation="MTWEYALIGLVVGIIIGAVAMRFGNRKLRQQQALQYELEKNKAE
                     LDEYREELVSHFARSAELLDTMAHDYRQLYQHMAKSSSSLLPELSAEANPFRNRLAES
                     EASNDQAPVQMPRDYSEGASGLLRTGAKRD"
     gene            92470..93837
                     /gene="degQ"
                     /locus_tag="NBI74_000215"
     CDS             92470..93837
                     /gene="degQ"
                     /locus_tag="NBI74_000215"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegQ"
                     /protein_id="HCD1930598.1"
                     /translation="MKKQTQLLSALALSVGLTLSASFQAVASIPGQVADQAPLPSLAP
                     MLEKVLPAVVSVRVEGTASQGQKIPEEFKKFFGDDLPDQPAQPFEGLGSGVIINANKG
                     YVLTNNHVINQAQKISIQLNDGREFDAKLIGSDDQSDIALLQIQNPSKLTQIAIADSD
                     KLRVGDFAVAVGNPFGLGQTATSGIISALGRSGLNLEGLENFIQTDASINRGNSGGAL
                     LNLNGELIGINTAILAPGGGSVGIGFAIPSNMARTLAQQLIDFGEIKRGLLGIKGTEM
                     SADIAKAFNLDVQRGAFVSEVLPGSGSAKAGVKAGDIITSLNGKPLNSFAELRSRIAT
                     TEPGTKVKLGLLRNGKPLEVEVTLDTSTSSSASAEMITPALEGATLSDGQLKDGGKGI
                     KIDEVVKGSPAAQAGLQKDDVIIGVNRDRVNSIAEMRKVLAAKPAIIALQIIRGNESL
                     YLLMR"
     gene            93927..94994
                     /gene="degS"
                     /locus_tag="NBI74_000216"
     CDS             93927..94994
                     /gene="degS"
                     /locus_tag="NBI74_000216"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane-stress sensor serine
                     endopeptidase DegS"
                     /protein_id="HCD1930599.1"
                     /translation="MFVKLLRSVAIGLIVGAILLVAMPSLRSLNPLSTPQFDSTDETP
                     ASYNLAVRRAAPAVVNVYNRGLNTNSHNQLEIRTLGSGVIMDQRGYIITNKHVINDAD
                     QIIVALQDGRVFEALLVGSDSLTDLAVLKINATGGLPTIPINPRRVPHIGDVVLAIGN
                     PYNLGQTITQGIISATGRIGLNPTGRQNFLQTDASINHGNSGGALVNSLGELMGINTL
                     SFDKSNDGETPEGIGFAIPFQLATKIMDKLIRDGRVIRGYIGIGGREIAPLHAQSGGM
                     DQLQGIVVNEVSPDGPAANAGIQVNDLIISVNNKPAISALETMDQVAEIRPGSVIPVV
                     VMRDDKQLTLQVTIQEYPATN"
     gene            complement(95057..95995)
                     /gene="mdh"
                     /locus_tag="NBI74_000217"
     CDS             complement(95057..95995)
                     /gene="mdh"
                     /locus_tag="NBI74_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000861594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HCD1930600.1"
                     /translation="MKVAVLGAAGGIGQALALLLKTQLPSGSELSLYDIAPVTPGVAV
                     DLSHIPTAVKIKGFSGEDATPALEGADVVLISAGVARKPGMDRSDLFNVNAGIVKNLV
                     QQVAKTCPKACIGIITNPVNTTVAIAAEVLKKAGVYDKNKLFGVTTLDIIRSNTFVAE
                     LKGKQPGEVEVPVIGGHSGVTILPLLSQVPGVSFTEQEVADLTKRIQNAGTEVVEAKA
                     GGGSATLSMGQAAARFGLSLVRALQGEQGVVECAYVEGDGQYARFFSQPLLLGKNGVE
                     ERKSIGTLSAFEQNALEGMLDTLKKDIALGEEFVNK"
     gene            96430..96900
                     /gene="argR"
                     /locus_tag="NBI74_000218"
     CDS             96430..96900
                     /gene="argR"
                     /locus_tag="NBI74_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001257852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ArgR"
                     /protein_id="HCD1930601.1"
                     /translation="MRSSAKQEELVKAFKALLKEEKFSSQGEIVAALQEQGFDNINQS
                     KVSRMLTKFGAVRTRNAKMEMVYCLPAELGVPTTSSPLKNLVLDIDYNDAVVVIHTSP
                     GAAQLIARLLDSLGKAEGILGTIAGDDTIFTTPANGFTVKELYEAILELFDQEL"
BASE COUNT        23740 a        26048 c        24316 g        22903 t
ORIGIN      
        1 gcaaatgcta atttaattag tactatttaa atattatttt gagcatatgc acataaggtt
       61 gcgcgctaaa gcacagattt gcgctttacc ttgccgggag gtactgcaat ccctgaaatg
      121 attgacattg atcacatttc tgcatttaaa ctcctgacat tcttatttca cccaatgaag
      181 ttatttattt ttaaatgaga ccaggtcctc attttaatag tccctggctg gagaatattg
      241 cacaatggcc aacatcgaaa tcagacaaga aacgccaact gcgttttata taaaagttca
      301 cgacacagat aatgtggcaa ttattgttaa tgataatggc ctgaaagcag gaacgcgttt
      361 tccggatggg ctggaattaa ttgaacatat tccccagggg cataaagtcg cattgctgga
      421 cattccggct aatggtgaaa ttattcgtta tggcgaggtg attggttacg ccgtgcgcgc
      481 aatcccacgc ggaagctgga tcgacgaatc aatggttgta ctaccggaag cgccgccgtt
      541 acatacgctg ccactggcaa ccaaagtccc ggaaccctta ccgccgctgg aaggatacac
      601 ctttgagggc tatcgcaatg ccgatggcag cgtgggcacc aaaaacctgc tcggcatcac
      661 caccagcgtc cactgtgtgg caggcgtggt ggactacgta gtaaaaatca ttgaacgcga
      721 tctgctaccg aaatacccga acgtcgatgg cgtggtgggg ctgaatcatt tgtacggttg
      781 tggcgtggcg attaacgcac ccgcggcagt tgtacctatt cgtaccattc acaatatttc
      841 gctgaaccct aactttggcg gcgaagtaat ggtgattggt ctgggttgtg aaaagttgca
      901 gcctgaacgc ctactgactg gaacggatga tgtgcaagct attccagtag aaagcgccag
      961 cattgtcagt ttgcaggatg aaaagcatgt cggttttcag tccatggtcg aggatatttt
     1021 gcaggtagcc gaacgccatc tacaaaaact gaatcaacgt cagcgagaaa cctgtccggc
     1081 ttcagaactg gttgtcggca tgcagtgcgg tggcagcgat gcgttttctg gcgtaacggc
     1141 aaacccggcg gttgggtatg cgtctgatct attggtgcgc tgcggcgcaa cggtgatgtt
     1201 ttcagaagtc acagaagtgc gtgacgcgat ccatctgctg acaccacgcg cagtgaacga
     1261 agaggtcggc aaacggctgc tggaggagat ggagtggtac gataactatc tcaatatggg
     1321 aaaaaccgac cgcagcgcca acccttcgcc gggcaacaag aaaggcggtc tggcaaacgt
     1381 ggtagagaag gcactcggct ccattgctaa atcgggtaaa agtgcaattg ttgaagtgct
     1441 gtcgcccggc caacgcccga ctaaacgcgg attaatttac gccgcgacgc cagccagcga
     1501 ttttgtctgt ggcacgcaac aggtggcttc gggtatcacc gtgcaagtgt ttacgaccgg
     1561 tcgtggtacg ccgtacggcc ttatggcggt acccgtcatt aaaatggcga cccgcaccga
     1621 gctggcgaac cgctggtttg atttaatgga tattaacgcg ggcactatcg ctaccggcga
     1681 agaaaccatt gaagaagtgg gctggaagtt gttccacttt attctcgacg tcgccagcgg
     1741 gaagaagaaa actttctcgg atcaatgggg actgcataac cagctggcgg tgtttaaccc
     1801 ggcaccggtg acctgatttc ttttggaacg tccctcgcaa aacatggcct tagtgccatg
     1861 tttttattgt ttaaagtcca cacatccatt aataatgcat ttgcattacc ttagttcaag
     1921 cttataattt gagcagaaaa caggctgtaa aaggacagta aataatgccc gctaatgctc
     1981 gctctaacgc tgtactgacc actgaatcaa aggtcactat acgcggacaa acaactatcc
     2041 ccgcgccagt gcgtgaggcc ttaaaactga agccaggcct ggacagcatt cattacgaaa
     2101 ttctgcctgg tgggcaagta tttatgtgcc gactgggaga tgaacaggag gatcatacta
     2161 tgaatgcatt tttgcgtttt ctggatgcag atatccagaa caatccgcaa aaaactcgtc
     2221 cattcgacat tcaacaagga aagaaacttg tcgctggcat ggacgtcaac attgacgatg
     2281 agattggcga cgacgaataa tggattttcc acaaagggtt aatggttggg cgctatatgc
     2341 tcatccctgt tttcaggaaa cctacgatgc tttagttgcc gaagtcgagg cattaaaggg
     2401 aaaagatcct gaaaattatc agagaaaagc tgccacaaag ttattggcgg tggtccataa
     2461 agtgattgag gagcatatca cggtcaatcc atcatcaccg gcattccgtc atggcaagtc
     2521 gttaggctct gggaaaaata aagactggtc acgggtaaaa tttggtgctg gtcgctatcg
     2581 tctcttcttt cgttacagtg aaaaagagaa agtcatcatt ctgggatgga tgaacgatga
     2641 aaacacgctg cgcacctacg gtaaaaaaac agatgcctat accgtattca gtaaaatgtt
     2701 aaaaagagga catcctcctg ccgactggga aagcctcacc caagagacag aagaaaacca
     2761 ttgatggtgt tcacaatgcc cgccgaacgc attggtgttg accagcgggc atcctttctc
     2821 attccccgac cagaatcact tcaaccccag cctttcgcag tccttccaga ctatccgcag
     2881 gaatgccttc atcaacaatg atcatgtcga tacgttgagt atcaatgatc ttatgtaaac
     2941 tggaacggtt gaacttactg gaatcggtga ccacgatgat ccgttccgca acttcgcaca
     3001 tccgacggtt taaacgggct tcatcttcat tatgcgtgct gacgccacgc tccagatcga
     3061 tcgcatctac accaagaaac agcatatcga agtggtaatt ttgcagcgat tgctcagcct
     3121 gatcaccgta aaaagattgc gactgacggc gcaaatgccc cccggtcatc agcagctcaa
     3181 cgccttccgc ttccagcaac gcattagcca cgttcatacc gttggtcatc gcaattacgt
     3241 cggtgtgctt gcgcatcaga cgagcaatct caaaagtggt ggtcccggag tcgaggatca
     3301 cccgatgacc aggctgaatc aactcaacgg cagctttcgc aacgctgcgt ttcatcgcgg
     3361 tgttcagtgc gcttttatct tccactgatg gctcgactga cggcgttgtg ctatcgcaga
     3421 tcaacgcgcc accataggca cgcacggcga tcccctgctt ttccagaaac gccagatcgt
     3481 tgcggatcgt cacagtagat acgccataca atgccgacag atcgttaacc tgcacactcc
     3541 cttgctgtcg cagacgctga atgatctgtt ctcgtcgctc gctggtgcct gtcactcgct
     3601 tctcacctga agcgtcggta ttactcatag taagtccttt cgtaaaactt tcgtttcatt
     3661 tcgttttgcc tattaacgcc tttctattaa gcaaatgcaa gcccacctta cccattggcg
     3721 caagctactc tcgtttcagt ggctttcatt atgtttcttt tgtgaatcag atcagaaaac
     3781 tattatcttt cgttttattt ttatctcacc atgacgcagt atcaactgaa acaaaacgaa
     3841 agattaatgt cgcagtaatc tgaactggag aggaaagtga aacatctgac agaaatggtg
     3901 agacagcaca aagcgggcaa aacaaatggg atttatgccg tttgttccgc acatccgctg
     3961 gtgctggaag ctgcaatccg ctacgccagt gcaaaccaaa cgccgttact gattgaagct
     4021 acctccaatc aggtggacca gtttggcggt tataccggaa tgacgcccgc cggttttcgc
     4081 ggctttgttt gtcagctcgc cgactcgttg aatttcccgc aggatgcgct gattctgggt
     4141 ggcgaccatc tggggccaaa ccgctggcaa aatctgcctg ccgctcaggc aatggccaat
     4201 gccgatgatt tgattaaaag ctacgtggcg gcaggattca aaaaaattca ccttgattgc
     4261 agcatgtcct gtcaggacga tccgattccc ttaaccgatg gcatcgtggc cgaacgcgcc
     4321 gctcgtttgg cgaaagtggc ggaagaaacc tgccgtgaac attttggcga agccgatctg
     4381 gagtatgtca ttggtaccga agtgccggtg cctggcggcg cgcatgaaac cttaagcgag
     4441 ctggcggtca ccacgccgga tgccgcccgc gccacgctgg aagcccatcg tcacgctttt
     4501 gaaaagcagg gtctgaatgc catctggcca cgcatcattg ccctggtggt tcaacccggc
     4561 gtcgaattcg atcacaccaa cgttattgat tatcagcccg ccaaagcgac cgccttaagc
     4621 cagatggtcg aaaactacga aacgctgatt ttcgaagcgc actctaccga ttatcaaacg
     4681 ccgcaatcgc tgcgccagct ggtgattgac cactttgcca ttctgaaagt tggcccggcg
     4741 ctgacctttg ctctgcgtga agctctgttc tctctggcag cgattgaaga agaactagtg
     4801 ccagcaaaag cctgttctgg tctgcgtcag gtgctggaaa acgtgatgct cgaccgcccg
     4861 gaatactggc aaagccacta ccacggtgac ggcaacgcgc gtcgtctggc gcgtggttat
     4921 agctactcgg atcgcgtgcg ctattactgg ccggacagcc agattgatga cgctttcgct
     4981 catctggttc gtaatctggc ggattcacca attccgctgc cgctgatcag tcagtatctg
     5041 ccgctgcagt acgtgaaagt tcgctccggc gagctgcagc caacgccacg ggaactcatt
     5101 atcaaccata ttcaggacat cctggcgcag taccacacag cctgtgaagg ccaataagca
     5161 aaacaaagag gaacacgcta tgccaaatat tgttttaagt cggattgatg aacgcttgat
     5221 tcacggtcaa gtcggcgttc agtgggtagg atttgcgggg gcaaatctgg tgctggtagc
     5281 caacgatgag gttgccgaag atccggtaca acaaaacctg atggaaatgg tactggcaga
     5341 agggatcgcc gtacgtttct ggacgctgca aaaagtcatc gacaacatcc atcgcgccgc
     5401 cgatcgacag aaaatcctgc tggtttgtaa aacaccggcc gatttcctga cgctggtgaa
     5461 aggtggcgtt ccggtgaatc gcattaacgt tggcaatatg cactacgcca atggcaagca
     5521 acaaatcgcc aaaacggttt ctgtggatgc gggtgatatc gcagcattta acgacctgaa
     5581 agccgctggg gtggaatgct tcgttcaggg cgtcccgaca gagcctgctg tggacctctt
     5641 taaattactt tgagggattc atcatggaaa tcagtctgtt gcaggcattt gcgttgggca
     5701 ttatcgcctt tatcgctggc ctggatatgt ttaacggctt aacccatatg caccgcccgg
     5761 tggttctcgg cccgttggtc gggctggtac ttggcgatct gcataccggt attttaaccg
     5821 gcggtacgct ggaactggtg tggatggggc tggccccgct ggcgggcgca cagccgccta
     5881 acgtgattat cggtactatc gtcggcacgg cgtttgccat tactactggc gtgaaacccg
     5941 atgtcgcggt cggtgtcgcc gtacctttcg ctgttgcagt acagatgggg attaccttcc
     6001 tgttctcggt gatgtccggc gtgatgtccc gctgcgaccg gatggcggag aacgccgata
     6061 ctcgcggcat tgaacgcgtg aactatctgg cactgctggc actcggcact ttctattttc
     6121 tctgcgcttt cctgcctatc tacttcggcg cggaacatgc aaaaaccatc attgatgtcc
     6181 tgccgcaacg attaattgat ggcctcggcg tcgcgggcgg catcatgcca gcaatcggtt
     6241 ttgccgtgct gctgaaaatc atgatgaaaa acgtctacat cccctacttc atcctgggct
     6301 ttgttgccgc agcctggctc aagttaccgg tgctggctat tgctgccgct gcgctggcga
     6361 tggcgctgat cgacctgctg cgtaaatctc ctgaaccgac tcaacctgcg gcacagaaag
     6421 aggaatttga agatggcatc taatcaaacc accctgccga acgtcagcga aaatgaagaa
     6481 acactgctga ctggcgtcaa tgaaaacgtg tatgaagatc agagcattgg cgcagagctg
     6541 acgaaaaaag atatcaaccg cgtcgcctgg cgctccatgc tgttacaagc gtctttcaac
     6601 tacgaacgta tgcaggcttc cggctggttg tacggtctgc tgcctgctct gaaaaagatc
     6661 cacactaata aacgggacct ggcgcgcgcc atgaaggggc atatgggttt cttcaatacc
     6721 catccgtttc tggtgacgtt tgttatcggc attatccttg cgatggagcg ttctaagcag
     6781 gacgttaaca gtattcagag caccaaaatt gccgtcggtg cgccgctcgg cgggattggc
     6841 gatgcgatgt tctggctaac gctactgccg atttgtggcg ggataggcgc aagcctcgcg
     6901 ctacaaggct ctattcttgg cgctgtcgtc tttattgtgc tgttcaacgt ggtgcacctg
     6961 gggctgcgtt ttggtctggc gcattatgct taccgcatgg gcgtggcggc tattccactc
     7021 attaaagcaa ataccaaaaa agtcggccat gcggcgtcta tcgttgggat gacggtgatc
     7081 ggcgcgctgg tggcaaccta tgttcgttta agcaccacgc tggaaatcac ggcgggcgac
     7141 gcagtggtta agttacaggc tgatgttatc gacaaactga tgcctgcctt cttaccgctg
     7201 gtctacaccc tgaccatgtt ctggctggta cgccgcggct ggagtccgct gcgcctgatt
     7261 gcagtgacag tggttctcgg catcgtcggt aaattctgcc atttccttta aatacaatga
     7321 ggtattagat gttaagtatt attttgacag ggcatggcgg atttgccagc ggcatggaaa
     7381 aagcaatgaa gcaaatcctc ggcgagcaat cgcagtttat cgccatcgat tttccagaaa
     7441 cctcaagcac cgcgctgctt acatcgcagc ttgaagaggc catcgcccaa ctggattgtg
     7501 aagacggcat tgtttttctg acggatttgc tgggcggcac gccgtttcgc gtagcttcaa
     7561 cgctggcgat gcaaaaaccg ggctgtgaag tgatcaccgg cacaaatttg caactattac
     7621 tggaaatggt gctggaacgc gaagggttaa gcggtgaaga gttccgtgtg caggcgctgg
     7681 agtgtggaca ccgtgggcta accagtctgg tggacgaact tggtcgctgc catgaggaat
     7741 gtccggtcga ggaaggaata tgacacacgt tctgcgcgcc agaaggctgc tgactgaaga
     7801 gggatggctc gatgaccatc agttgcgtat tgctgacggt gtaatcgcag caatcgaacc
     7861 gattccagtg ggcgtgactg aacgcgatgc ggaactgctc tgccccgctt acatcgacac
     7921 ccatgtacac ggtggtgcgg gcgttgatgt aatggatgac gcgccggatg cgctcgacaa
     7981 gctggcaatg cacaaggcac gcgaaggtgt cggcagttgg ttaccgacca ccgtaaccgc
     8041 gccgcttaat accattcatg cggcgctgaa acgtattgct ctacgctgcc agcgcggtgg
     8101 gcctggtgcg caagtgctgg ggagttatct cgaaggaccg tacttcacgc cgcagaataa
     8161 aggcgcacat ccgccggagc tgtttcgcga gctggaaatt gccgagctgg atcaattgat
     8221 tgccgtttct cagcagacct tacgcgtggt agcgctggca ccagaaaaag aaggggcatt
     8281 gcaggccatc cgccatctta aacagcaaaa cgtacgggtg atgctggggc atagcgcggc
     8341 gacctggcaa caaacccgtg ccgcgtttga tgctggtgcc gacggcctgg tgcattgcta
     8401 taacgggatg acaggtttac atcaccgcga accgggaatg gttggcgcgg gattaacgga
     8461 caagcgcgcc tggctggaac tgatagccga tggtcatcat gtacatccgg cggcaatgtc
     8521 gctctgttgt tgctgtgcga aagagaggat cgtactgatc accgacgcga tgcaggcagc
     8581 cgggatgccg gatggtcact atacgttatg tggtgaagaa gtgcagatgc acggtggcat
     8641 tgtccgtacc gcgttcggtg ggctggcggg cagtacgctg tctgtcgatg cggcagtgcg
     8701 caacatggtc gagttgacgg gcgtaacgcc tgcggaagcc atccatatgg cgtcgctgca
     8761 tccggcgcga atgctgggtg ttgatggtgt tctgggatcg cttaaaccgg gcaaacgcgc
     8821 cagcgtcgtt gcgctggata gcgggctgca tgtgcaacaa atctggattc agggtcaatt
     8881 agcttcgttt tgatggtttg ctcctttatt aggccttcac ttcccccgta aggcctttct
     8941 ttttctttct ttttgatctg tgcagcggtg tcggatgcga cgctaatgcg ccttatccga
     9001 cctacggttg gtgaccgtaa ggccggataa agtatttacg ccgcatccag caatcccttt
     9061 tacttccctt gtcttttctt tcaacgatca cagatttcgt tttatttcct ttttctccat
     9121 tgaactttca gtttcttttc tatagatttt aaccaacgaa agaaatcacc aagtgaaatg
     9181 aaacgaaagg caagcgaaag cgacaacgcc gacgtcaagt tcatcagact aaggattgag
     9241 ttatgccaga aaattacacc cctgctgctg ccgcaaccgg tacatggact gaagaagaga
     9301 tccgccatca gcctcgcgca tggatccgtt cactcaccaa cattgacgcg ctacgttccg
     9361 cgctcaataa cttccttgaa ccgttactgc gcaaagagaa tctgcaaatc atcctgaccg
     9421 gagccggaac atcggcattt atcggtgaca tcatcgcgcc gtggctcgcc agccataccg
     9481 gtaaaaactt cagcgccgta ccaaccaccg atctggtcac caatccaatg gactacctga
     9541 acccagctca tccgctactg ttgatctcct tcggtcgctc cggcaacagc ccggaaagcg
     9601 tcgccgccgt ggaactggca aatcaatttg taccagaatg ctatcacctg ccgatcacct
     9661 gcaacgaagc gggcgctctt taccaaaacg cgatcaacag cgacaacgcg tttgccctgc
     9721 tgatgcccgc agaaacgcac gatcgcggct ttgcgatgac cagcagcatt accaccatga
     9781 tggccagctg cctcgcggtt ttcgcacctg agacgatcaa cagccaaacc ttccgcgacg
     9841 tggcggatcg ttgccaggcg atcctgacct cactgggcga tttcagcgaa ggtgtgtttg
     9901 gttacgcacc gtggaaacgg atcgtttatc tcggcagcgg tggcttacag ggcgcagcac
     9961 gcgagtcggc gctgaaagtg ctggaactga cagcgggtaa actggcggcc ttctatgatt
    10021 ccccgaccgg attccgtcat ggcccgaaat cgctggtcga tgacgaaacg ctggtagtgg
    10081 tgtttgtctc cagtcaccct tacacccgtc agtacgatct tgacctgctg gcagaactcc
    10141 gccgtgacaa ccaggcaatg cgcgtgatcg ccatcaccgc tgaaagcaac gacgttatcg
    10201 ccgctggccc gcatatcatc ctgccgccgt cccgtcactt tatcgacgtt gaacaagcat
    10261 tttgcttcct gatgtacgcc cagacgtttg cactgatgca gtcgctgcac atgggcaata
    10321 cgccggatac cccatcagcc agtggcaccg taaaccgcgt ggtgcaaggc gtaactattc
    10381 atccgtggca ggcataagag gatcgcatta tgagcattat ctccaccaaa tatctgttac
    10441 aggacgccca ggccaatggc tacgcggtgc ctgcctttaa catccataac gccgagacga
    10501 tccaggcgat cctcgaagtg tgcagtgaaa tgcgatcgcc ggtgatcctc gccgggacac
    10561 caggtacctt taagcatatc gcgctggaag agatctacgc cctgtgtagc gcctattcca
    10621 cgacctataa catgccgctg gcactgcatc tcgaccacca cgaatcgctg gatgatattc
    10681 gccgtaaagt ccacgcaggc gtgcgcagcg cgatgatcga cggcagccac ttcccgtttg
    10741 ccgagaacgt gaagctggtg aaatcggttg tcgacttctg ccactcccgg gattgcagcg
    10801 tggaagcaga actgggccgc ctcggcggtg ttgaagatga catgagcgtt gacgccgaaa
    10861 gtgcattcct gaccgatcca caagaagcta aacgctttgt cgaactgact ggcgtcgaca
    10921 gcctggcggt agcgattggt acggcgcacg gcttatacag caaaacgccg aagattgatt
    10981 tccagcggct ggcggaaatt cgtgaagtgg tggatgttcc tctggtgctg catggtgcca
    11041 gcgatgtccc ggatgaattt gtccgtcgca ctattgaact cggtgtaaca aaagtgaacg
    11101 ttgccacaga attaaaaata gccttcgctg gcgcggttaa agcctggttt gcggaaaatc
    11161 cgcagggtaa tgatcctcgt tattatatgc gcgtcggaat ggatgcgatg aaagaagtgg
    11221 tcagaaataa aattaatgtc tgtggttcag cgaatcgaat ttcagcataa tcattaattt
    11281 tatacgtttt ttcagaatgc actgcccgat taatatcggg tagtacctta atggcaaagg
    11341 agataaccaa acattaataa atacatcagt acaatatcgc aacaataata tatttaaaaa
    11401 tattatatta ttcaacttta tggtgaggat tacacaatga gcagtccaaa tattctctta
    11461 acccgtattg ataaccgtct ggttcacggt caggttggcg tgacctggac atccaccatc
    11521 ggtgcgaatc tgttggtagt cgtggatgat gttgtcgcta acgatgatat tcaacagaaa
    11581 ttaatgggta ttaccgcaga aacctacggc tttggcattc gtttctttac tatcgaaaaa
    11641 accattaacg tcatcggcaa agctgcacca catcagaaga tcttcctgat ttgccgtacg
    11701 ccacaaacgg tacgtaaatt ggtagaaggt ggtattgacc tgaaagatgt caacgtcggc
    11761 aatatgcatt tctcggaagg gaaaaagcaa atcagcagta aagtttatgt cgatgaccag
    11821 gatctcacgg acttacgtta tattaaacaa cgtggcgtga atgttttcat tcaggacgtt
    11881 cctggcgatc aaaaagaaca aatccctgac taaatctgaa atcgccttaa tattggtttg
    11941 aggtaataaa aatgcatgaa ataaccctac tccagggatt atccctggcg gcgttagttt
    12001 tcgttctggg gattgatttt tggctggaag ccttattttt attccgcccg ataatcgttt
    12061 gtacccttac aggtgctatt ctcggtgata ttcagactgg cttaattacc ggaggcctga
    12121 cagagttggc tttcgccgga ttaacccctg caggtggtgt tcagccgccc aaccctatta
    12181 tggcgggtct gatgaccacc gtcattgcat ggtctacggg cgttgatgcc aaaacggcaa
    12241 ttggtcttgg cctgccgttt agtttgttaa tgcagtacgt cattctgttc ttctattccg
    12301 ctttctcatt atttatgacc aaagccgata aatgcgcgaa agaggcggat acggcagcgt
    12361 tttcccgact taactggaca acgatgctca tcgtcgcttc agcgtatgcg gtgattgctt
    12421 tcctctgtac ttacctggca caaggggcga tgcaggcgct ggtgaaagcg atgcccgcct
    12481 ggctgaccca cggctttgaa gtggctggcg gtattctgcc tgccgttggt tttggcttgc
    12541 tgctgcgcgt gatgttcaaa gcgcaatata tcccttacct gatcgccggt ttcctgtttg
    12601 tttgctacat ccaggtcagc aacctgttgc cggttgccgt gctgggcgca ggctttgcgg
    12661 tgtatgagtt tttcaatgcg aaatcccggc agcaagcgca accgcagccc gttgccagta
    12721 aaaatgaaga agaggactac agcaatggga tctgaaatca gtaaaaaaga tatcacccgt
    12781 ctgggctttc gttcgtcact gctacaagca agctttaact acgaaaggat gcaggcgggc
    12841 ggttttacca gggcgatgtt gccgatcctg aaaaagattt ataaggacga caaaccgggc
    12901 ctaagcgcgg cgatgaaaga taacctcgaa tttattaata cccaccctaa tctggtcgga
    12961 tttctgatgg ggttattaat ttcgatggaa gaaaaaggag aaaaccgcga caccattaaa
    13021 ggcctcaaag tggcactgtt tggcccaatc gccgggattg gcgatgcgat tttctggttt
    13081 actttgttgc cgattatggc ggggatttgc tcatcgtttg ccagtcaggg gaacctgttg
    13141 ggaccgattt tgtttttcgc cgtttacctg cttatctttt tcctgcgcgt cggctggacc
    13201 cacgtcggtt attcagtcgg cgtgaaggct attgataaag tgcgagagaa ctcgcagatg
    13261 attgcccgtt cggcaaccat cctcgggatc acagtaatcg gcgggctgat cgcttcgtat
    13321 gtgcatatta acgtggtgac atcgtttgcc atcgacagta cccacagcgt cgcgctgcag
    13381 caggatttct tcgataaagt cttcccgaat attttaccga tggcctacac cctgctgatg
    13441 tattacttcc tgcgggtgaa aaaagcgcat ccggtgctgt taatcggcgt gacgtttgtt
    13501 ctttctattg tttgttctgc attcggcatt ttgtaaatgg aacgaggcac cgcgtctggc
    13561 ggtgcctctt tacttaagga atttcatcct gtgcaaaccc ttcaacaagt tgaaaactat
    13621 acggcgttaa gtgaacgtgc cagcgaatat ttattggccg tgatccgtag caaaccggat
    13681 gccgtgattt gcctggcgac cggagccacg ccattactga cgtatcatta tttggtagaa
    13741 aaaatccacc agcagcaggt tgatgtcagc cagctcacct tcgtgaagct cgacgaatgg
    13801 gtggatctgc cattaacgat gcctggtacc tgcgaaactt tccttcaaca gcatatcgtg
    13861 cagccgctgg ggctacgtga agaccagctc atcagctttc actccgaaga gataaatgag
    13921 acagagtgcg aacgggtaac gaacctgatt gcgcgcaaag gcggtctgga cttatgcgtt
    13981 ctcggattgg ggaaaaacgg tcatcttggg ctgaacgaac cgggagaaag cctgcaaccg
    14041 gcctgccata tcagtcaact tgatgccaga acacagcaac atgagatgtt aaaaaccgct
    14101 gaccgtcccg tgactcgtgg gatcacctta ggcctgaagg atattctcaa tgcccgcgaa
    14161 gttttgttac tgataactgg cgaaggaaaa caggatgcga cagaacgttt tctcacggct
    14221 aaagtctcca ccgctatccc ggcttcattt ttatggctgc ataaccattt cacatgcgtg
    14281 attgatgaaa tgtgtcgtcg ttaactcgcc tggtacaggc gttgcagcgc tattcctgct
    14341 gcgacgcctg taatcactct ccctgctgct ccagcgcata cttatacagc gcatttttct
    14401 tcacgccgtg aatttctgcg gccagcgccg ccgctttttt cagcggcagt tctgcctgta
    14461 acagcgccag cgtgcgcagg gcatcagcag gcaggtcatc ttcctgcgct ttatggcctt
    14521 cgacaatcag caccatttcg cctttgcgac ggttttcatc ttcttttacc cacgccagca
    14581 gctcgccaac gggcgcgccg tgaatggttt cccaggtttt ggttagctca cgcgccagaa
    14641 ccacgtaacg ggattcgcct aataccgcaa caatatcttc caggctatct aacagacggt
    14701 gggtagattc ataaaaaatc agcgtgcgcg gctccgcttc aatagctttt agcgcatcac
    14761 ggcggccttt tgatttggca ggtaaaaagc cttcataaca aaaacgatcg gatggtaaac
    14821 ccgcggcgct taacgcagtg atagcagcac acggccctgg tagcggcacc acgcgaatcc
    14881 ccgcttcacg acaggtacgc accagatggt agccaggatc gttaattagc ggcgttccgg
    14941 catcggaaac cagcgcaatg ttttgcccct cttgcagctt cgccagcagc gtttcggctt
    15001 tttgttgttc gttatgatcg tgcagcgcaa acaaccgggc attaatccca aaatgttgca
    15061 gcaataaacc ggtgtgacgc gtatcctcgg cggcaatcag atcaacggcc tgtaatacct
    15121 ctaatgcacg ctgggtgata tccgccagat tgccgattgg cgtcggtaca atgtaaagct
    15181 gcccctgaga attatccgcc gattggtgtt gtttcattgt gtcgtccgta ttgccgattt
    15241 aatattgagc attgcgtaaa aaaaatatca ctggatacat tatggtaccc tcaacatttt
    15301 ctcgtttgaa agccgcgcgt tgtctgcctg ttgttttggc agccctgatt ttcgccggtt
    15361 gtggcaccca tactcccgat cagtccactg cttatatgca gggcacggcg caggctgatt
    15421 ctgcttttta cctgcaacag atgcagcaaa gttctgatga taccaggatc aactggcaat
    15481 tactcgccat tcgtgcactg gtgaaagaag gtaaaaccgg gcaggcggtt gagttggtta
    15541 accaactacc gcaagaactg aatgataatc agcgtcgcga gaaaacactg ctggcggcag
    15601 agattaaact ggcgcagaaa gattttgctg gcgcgcaaaa cttgctggcg aaaatcacac
    15661 ctgccgattt agaacaaaac cagcaagccc gttactggca ggcaaaaatc gatgccagcc
    15721 aggggcgtcc ttccattgat ttactgcgcg cgttaattgc tcaggaaccg ctgctcggtg
    15781 cgaaagaaaa gcagcagaat attgacgcca catggcaggc gctctcctcc atgactcagg
    15841 agcaggcgaa tacgctggtg atcaacgccg acgaaaatat tctgcaaggc tggctggatt
    15901 tgcagcgcgt ctggtttgat aaccgtaacg atcctgacat gatgaaagct gggatcgccg
    15961 actggcagaa acgttatccg aacaacccgg gcgcgaaaat gctgccaacg cagttggtta
    16021 acgtaaaagc gtttaaacca gcatcgacca acaaaatcgc cctgctgttg ccgctgaatg
    16081 gccaggcagc ggtatttggt cgcactattc agcaaggctt tgaagcggcg aaaaatatcg
    16141 gcactcagcc agtggcggcc caggtagctg ccgcacctgc cgcagacgta gcagaacaac
    16201 ctcagccgca aaccgtggat ggcgttgcca gcccggcaca agcttcggtt agcgatctga
    16261 ccggtgaaca gcctgcggcc cagccggtac ctgtaagcgc cccggcgaca agcaccgcag
    16321 cggtaaacgc acccgcaaat ccatccgcag agctgaaaat ctacgatacc tcatcacaac
    16381 cacttagcca gatcttaagc caggttcagc aggatggtgc gagtattgtg gtcggtccgt
    16441 tgctgaaaaa taacgttgaa gagttgctga agagcaacac cccgctgaac gtgctggcac
    16501 tgaaccagcc ggagaatatc gaaaaccgcg tcaatatttg ttacttcgcg ctttcgccag
    16561 aagacgaagc gcgcgatgca gcgcgtcata ttcgtgacca gggtaaacaa gcgccgctgg
    16621 tgctgatccc gcgcagttca ttgggcgatc gcgtagccaa tgcgtttgcg caagagtggc
    16681 agaaactggg tggcggcacc gttctgcaac aaaaatttgg ttccaccagc gaattacgcg
    16741 cgggtgttaa cggcggttct ggtattgctt taacgggtag cccgattact cccagagcga
    16801 caaccgactc cggcatgacg accaacaatc caacgctgca aaccacgcca accgatgacc
    16861 agttcaccaa taatggcggt cgtgtcgatg cggtgtacat tgtggcaacg ccgggtgaaa
    16921 tcgcttttat caaaccgatg atcgccatgc gtaacggtag ccagagcggt gcaacgctgt
    16981 acgccagttc ccgcagtgcg caagggaccg ctggcccgga tttccgcctg gagatggaag
    17041 gcttgcagta cagcgaaatc ccgatgctgg caggcggtaa tctgccgtta atgcagcagg
    17101 cgctcagcgc ggtgaacaac gattattcgc tggctcgcat gtatgcgatg ggtgtcgatg
    17161 cctggtcgtt ggcaaatcat ttctcacaaa tgcgccaggt tcagggtttt gaaatcaacg
    17221 gtaataccgg aagcctgacg gcaagcccgg attgcgtgat taacaggaag ttatcatggc
    17281 tacagtacca acaaggtcag gtagtccccg ccagttaacc accaaacaga ccggcgatgc
    17341 gtgggaagca caagcgcgtc gctggctgga aggcaaagga ctacggttta tcgccgctaa
    17401 cgtgaacgag cgtggcggcg agatcgatct gataatgcgt gaaggccgga ccaccgtttt
    17461 tatcgaggtg cgttaccgcc gctctgcgct ttatggcggc gcggcagcca gtgtgacccg
    17521 cagcaaacaa cacaaattat tacagactgc ccgtttgtgg ctcgcgcgtc ataatgggag
    17581 ttttgatact gtggattgcc ggttcgatgt ggtagccttc accgggaatg aggttgagtg
    17641 gattaaggat gcctttaatg accactcata attaaggttt aaggattagc gtgcaagaaa
    17701 gaattaaagc ttgcttcact gaaagcattc aaactcaaat tgcggcggca gaggcgcttc
    17761 cggatgccat ctcccgtgca gccatgacgc tggttcagtc tctgctcaat ggcaacaaaa
    17821 tcctctgttg tggtaatgga acttccgctg ccaatgcaca gcattttgct gccagcatga
    17881 tcaaccgttt cgaaacggag cggcccagct tacctgccat tgcactaaat actgataatg
    17941 ttgtcttaac ggcaattgcc aacgatcgct tacatgatga agtgtatgca aaacaggtgc
    18001 gggcgctggg tcatgcggga gatgtattgt tagccatttc cacccgtggc aacagccgcg
    18061 atattgtaaa agcggtagaa gctgccgtta cgcgtgatat gaccattgtg gcattaaccg
    18121 gctatgacgg cggtgaactt gcaggtttgt tagggccaca ggatgtggag atccgcattc
    18181 cttcgcatcg tagtgctcgc attcaggaaa tgcatatgct gacggtaaat tgcctgtgcg
    18241 atctgatcga taacacgctt ttccctcacc aggatgttta aggagaatac atgaaggcat
    18301 tatcgccaat cgcagtcctt atttccgcgc tgctgttgca aggttgtgtt gccgctgccg
    18361 tagtgggtac tgctgctgtg ggtaccaaag ccgcaactga cccacgcagt gtcggcaccc
    18421 aggtagacga tggtaccttg gaagtgcgcg tgaacagcgc attgtcgaaa gacgaacaaa
    18481 ttaagaaaga agcgcgcatt aatgtaacgg cctatcaggg caaagtgctg ctggttgggc
    18541 agtcaccaaa cgctgaactt tcggctcgcg ccaaacagat tgctatgggc gtagacggtg
    18601 ccaacgaagt gtataacgag attcgtcagg gccagccgat tggtctgggc gaagcgtcta
    18661 acgatacgtg gatcaccacc aaagtacgtt cgcagctgtt aaccagcgac ctggtgaaat
    18721 cgtccaacgt gaaagtgacc accgaaaacg gtgaagtgtt cctgatgggg ctggtgactg
    18781 aacgtgaagc gaaagcggcg gcagatattg ccagccgggt aagcggcgtg aagcgggtaa
    18841 ctacggcgtt tacgtttatt aaatagcagc ccttgtaatg cctgatgcga cgcttgcagc
    18901 gtcttatcag gcctacaacg atgcaaaccg taggtcggat aaggcgttta cgccgcatcc
    18961 gacatgtttt ccctcaaatc aaaccaacag cgccagcccg ccgacaatca caccagacac
    19021 tgccaccatc gcccccgtca gccataaggc tttcgccggg aacgctttgc gcagcataat
    19081 cagtgacggc aaactcaccg ccgggagcgt catcaacaat gccagcgccg gagcagttcc
    19141 catacctgcc agcatcatcg tttgtacaat cggaatttct gctgccgtgg gaatgacaaa
    19201 caagcatcct gccaccgcca tcgccaccac ccacatcagg ctgttatcga cagcaccatc
    19261 ggcatggggg aataaccaga cgcgagcggc acccaacacc agtactgcaa ggatgtaaac
    19321 cgggatcgta ctccagaaaa gcgtccatag cgccctgccc cagcggctaa aaaatcctcc
    19381 ctgtgcttcc ggtatatcaa tttcgaccgg tgcctgcgtt tgcggtgttt cacgcaccca
    19441 tttttgcacc agcgtcgcaa tcagcaacac catcaccagc cccgccacca gacgaatcgc
    19501 cgcaaaaccc cagccgagga cgaaccccat aaacaccagc gtcgccgggt ttaacaacgg
    19561 attccccatc cagaatgcca gcgcaccgcc catcgacacc tgttggcgac gcattcccgc
    19621 cgcgaccgga gccgcacagc aggtacacat catacccggc agcgaaaaca gcgttcccag
    19681 cagcgtgccg cgaaagcgcg attgcccaag cgtacgcaac aaccagtcac gcgggatcag
    19741 tacctgaatc aacgaaccga ggatcacccc cagcaccgcc gctttccata ccgcgaggaa
    19801 atagatcatc gcgtaatcca acgccgcctg ccacggatta gcatccgcct gcgcaaggat
    19861 agatttaccg atactgtggg tttcggcagc agtaaacgct ttgccgtagt aaggttgcca
    19921 tttcacatac cagagaccgg caatgacaac gagaaagaaa agcgcgggtt tccaccactg
    19981 aatgggcgtt gccgcctgag atgaagactg accagtcata gcattcccca ggaataagtt
    20041 atgataattg agcgcgtgaa tattacgctc actatcaatt cttgggaata attattcagc
    20101 tcttttgcgt aattctgaag agcttaaaat cgtcacacct tcatgctcag gccgcatcgc
    20161 ctctgccagc atgacccgcg ccacgtctct cgcatcaatg gatttccagt taccgggtaa
    20221 caaacggaac agcggcgcaa aaagcgtttc gttcatccgc tgtttgctac gatcacccag
    20281 taacatcgac gggcgagcaa tggtcaattt cggccagttc tgggcgatta atgcttcttc
    20341 catctcccct ttgacgcggt tatagaaaaa cggcgagtgg gcattagcac ccatagcact
    20401 gaccaccagc atatgctgcg caccgagccg ccgcccggtt aatgcggtat ccaccaccaa
    20461 cgtgtaatcg gcatgaataa acgcctcttt gctccccgct tctcgccgcg tggtgccgag
    20521 acaacaaaac acaatgtcga tgggatcggt gacctgcgcc agcgcgtcag tcagttgcgg
    20581 atcatgggga ttaaacaccc cgggcatatc gcccaacgga cgtcgcgtcg gcgcagcaat
    20641 gacgttaact ttcggttcat taatcaacat ccgcagcagg tgaccgccca ccagccccgt
    20701 tgcgcctgta atcagtacct gactcatctt cgctccttta cagaattgtc tgccttgcgc
    20761 tccccagctc atgcaccagg cttaatagac catgaggtaa ttatccccga ttgtggaaaa
    20821 ttcgcctcat ccaatgcaac aacgcggagg aagcatgagt aagaaaatcg ccgctttaat
    20881 cactgatgaa tttgaggatt cagaattcac ttcacccgca gacgagttcc gtaaagccgg
    20941 acacgaagtg attaccattg aaaaacaagc gggtaaaacg gtgaaaggca aaaaaggaga
    21001 agccagcgtg gtcatcgata aatccatcga tgaagtgacg cccgcagagt tcgatgccct
    21061 gctgctaccg ggcggtcatt caccagatta tctgcgtggc gacaaccgtt ttgtcacctt
    21121 cactcgcgat tttgtgaaca gcggcaaacc tgttttcgcc atctgtcacg gtccccagtt
    21181 gctgatcagc gccgatgtga tccgtggtcg taagctgacc gccgttaaac cgatcgtgat
    21241 tgatgttaaa aacgcgggcg cggaatttta cgatcaggaa gtggtggtcg ataaagatca
    21301 gctggttacc agccggacac cggacgatct gccagcgttt aaccgcgagg cgttacgcct
    21361 gctcggtgcc tgagttacgt agccaaatca tttttttacc aaagcccagc gtgttgtcgg
    21421 tgaatttgat ttcatcgagc cggatttccc ataccggggc cgacagcatt ctggcaaccg
    21481 gaaagcgacg attgtacgcc ttgcgtgcga ggtcgctttc ctcgccttcc agtctgcgga
    21541 tctcaccttt aaactgcacg ccgcgaatta acgctaccgt tttcggctga ccgtttaccg
    21601 ttccggcaat tgccgcctgc ggcccgctca tctgcgcatg acgcgttttt tcttccgtca
    21661 aaatgtagaa ggcgactttc tgcgcatcaa aaagataaaa ggcattggcg caccacagtt
    21721 ccccttcctg ctgcacacac caggtgacaa catgttgttt tgccagccaa cggctgatgg
    21781 cggtgagtgt ttccattgct gttctctttt atactgtggg cctgacctta acatgcgacg
    21841 cagacgatga caccctggtt tctttacttg atccgtaccg ccgacaataa gctttatacc
    21901 gggatcacca cggatgtcga acgccgctat cagcagcacc aaagcggcaa aggggcgaaa
    21961 gcactgcgag ggaaaggaga actaacgctg gcgttttccg cgccagtggg cgaccgttcg
    22021 ctggcactac gagcggaata tcgcgttaaa caactgacta aacggcaaaa agagcgtctg
    22081 gtaacggaag gcgcagggtt tgcagaactg ttgagcagcc tgcaaccccc ggagattaaa
    22141 agcgattgaa atgctcgtga tactcgacca gaccagttac gccgttcagc gcgtcatccg
    22201 ctaaacgatg tacctggaag gcgctttcag tgcccggcca gcggcaatgc agatcgtgat
    22261 gcgccgccag ttcaaagccg aaacggctgt acagcgccgg atcgcccagc gtcaccactg
    22321 cggcatagcc gaactcatta agcgaatcga gtccttcata gaccagttgg cgtgccagcc
    22381 cctgtccacg gtatttttca tcaaccgcca gcggtgccat gccgacccat tgcaggtctt
    22441 cgccctgcac atcaaccgga ctaaaagcca catagccaat gacctgacct tcgtcatctg
    22501 tcgccaccag ccccagcgtc agaaagccat cttcacgcag atcgtgaacc agcttcgctt
    22561 ccgcatcact ttcgaatgaa cgacgcagca gggcatcaat acccggcgca tcaatgggaa
    22621 tttctactcg aattagcatg gttcacctac cgatgtctgt ttggtttcag gcgcggtttt
    22681 cattcccgcc tcaacaaaat ccgccagttg cagcagcatc atgcgtaagg ctttcggcat
    22741 ttgctccagt tcaatggcgt ccatcaggtt tttcacatac agccccagct ccgtatcgcc
    22801 ttcaatcacc agccgacgct ggaagaagag cgtatccgga tcttgtttac gcgccgcaat
    22861 catcagcaga tcgctggcgt cggcactaaa actcacatca gcttgcgcgt tctggctaac
    22921 gaccagtttg ccattcacca ccgaggtaaa ccattgcagg tcaatatcac gcacatgaat
    22981 acttaaccag cgaccttcaa gaaactccag ctcgccatca tccagcgcct gacggaattg
    23041 ccagcttaag acctgctcaa gaacctggcg ttttagcgca aatggcgtca gttttaccgg
    23101 tacactcaac agagatggcc ccaaatgcac aatacgggaa cgcagtttat ccaacacgag
    23161 ttttactccc tgtttcaaca atcatcctat tttgccatat cagaaaaata acatagcggt
    23221 ataaatcaac aattccatat gaaattgctg ctaccaccaa tacaacttta actgccttaa
    23281 atcaaaaatt gtcgcagcaa ggttaactaa aatcccagtt cgttaacatt tttgcgtttt
    23341 gatagcgcaa ccttcaggaa aaattatgga gctgctctgc cctgccggaa atctcccggc
    23401 gcttaaggcg gccatcgaaa acggcgcaga tgctgtttat atcgggctaa aagatgatac
    23461 caatgcccgt cacttcgccg gccttaactt taccgagaaa aaattgcagg aagcggtgag
    23521 ttttgtccat caacatcgcc gcaaacttca catcgcgatt aacacttttg cgcatccgga
    23581 cggttacgcc cgttggcagc acgccgtgga tatggcggcg caactgggtg ccgacgcgct
    23641 gatcctcgcc gacctcgcca tgctggagta cgccgccgaa cgttatccgc atattgagcg
    23701 ccacgtatcg gtgcaggctt cggcgaccaa tgaagaggcg attaactttt atcatcgcca
    23761 ttttgacgtt gcccgcgtgg tactgccgcg cgtgttgtcg attcatcagg tgaaacagct
    23821 ggcacgggtc acacctgtac cactggaagt gtttgctttc ggcagcctgt gcattatgtc
    23881 ggaaggccgt tgctatctgt cgtcgtatct gacgggtgaa tcgcccaaca ccgtgggggc
    23941 atgttctccg gcccgtttcg tgcgctggca gcaaacgccg caagggctgg aatcccgcct
    24001 gaacgaagtg ctgatcgacc gttatcagga cggcgaaaac gcaggttatc cgacgctgtg
    24061 taaagggcgt tatctggtgg acggcgagcg ctatcacgcg ctggaagaac caaccagtct
    24121 caataccctg gaactgctgc cggagttaat ggcggcgaat attgcttcgg tgaaaattga
    24181 aggccgccag cgtagcccgg cgtatgtcag ccaggtggcg aaagtctggc gtcaggctat
    24241 cgaccgttgt aaggccgatc cgcaaaactt cgtaccgcaa agcgcgtgga tggagacgct
    24301 cgggtcgatg tccgaaggca cgcaaactac ccttggcgcg tatcaccgta aatggcagtg
    24361 agaaaagcaa tgaaatattc cttagggccg gtgctatggt actggccaaa agagacgctg
    24421 gaagactttt atcagcaggc cgccgccagc agcgcagacg tgatttatct tggtgaagcg
    24481 gtatgcagca agcgccgggc aaccaaagtt ggcgactggc tggagatggc aaaatcactc
    24541 gccggaagtg gtaagcagat tgtgctctcc acgctggcgc tggtgcaggc atcctctgaa
    24601 ctgggcgaac tgaaacgcta tgttgagaac ggtgagtttc tgattgaagc cagcgatctc
    24661 ggcgtggtga atatgtgcgc tgaacgcaaa ctgccgttcg tcgccgggca cgcgctgaac
    24721 tgctacaacg cggtgacgct gaaaatattg cttaaacagg gcatgatgcg ctggtgtatg
    24781 ccggtggagc tttcccgcga ctggctggtg aatctgctta atcagtgcga tgagttgggc
    24841 attcgcaacc agtttgaagt ggaagtcctg agctacggtc atctaccgct ggcctactcc
    24901 gcacgctgct ttaccgcgcg ttcggaagac cgcccgaaag atgagtgtga aacctgctgc
    24961 attaagtatc cgaacgggcg caacgtacta tcgcaggaaa accaacaagt gtttgtactc
    25021 aacggcattc agaccatgag cggctacgtt tacaacctcg gtaacgagct ggcatccatg
    25081 caggggctgg tcgatgtggt ccgcctgtca ccgcagggta ctgacacttt cgcaatgctc
    25141 gatgccttcc gcgctaatga aaatggcgct gcgccactac cgttgacggc aaacagcgac
    25201 tgtaacggct actggcggcg gctggccggg ctggagctgc aagcgtaagt atatagctca
    25261 ctttgttaac aactttaact actctttaat gcagtattaa agactaatcg gtaacaaagt
    25321 gagctgttat gactgataaa accattgcgt tttcgctact cgatctggcc cccatccccg
    25381 aaggttcttc agcgcgggaa gcgttctccc actctctcga cctcgcccgt ctggctgaaa
    25441 agcgcggcta tcatcgctac tggctggcag aacaccacaa tatgaccggc attgccagtg
    25501 ctgcaacgtc ggtgttaatt ggctatctgg cggcgaatac caccacgctg catctggggt
    25561 ctggcggcgt gatgttgcct aaccactcac cgttggtcat tgccgaacag ttcggcacgc
    25621 ttaatacgct ctatccgggg cgaatcgatt tggggctggg tcgcgcgccg ggtagcgacc
    25681 aacggacaat gatggcgcta cgtcgtcata tgagcggcga tattgataat ttccctcgcg
    25741 atgtcgcgga actggtggac tggtttgatg cccgcgatcc caatccgcat gtgcgccctg
    25801 taccgggcta tggcgagaaa atcccggtgt ggttgctagg ctccagcctt tacagtgcac
    25861 aactggcggc gcagcttggt ctgccgttcg cgtttgcctc acacttcgcg ccggatatgc
    25921 tgttccaggc gctgcatctt tatcgcagca acttcaaacc gtcggcacgg ctggagaaac
    25981 cgtatgcgat ggtgtgcatc aatattattg ccgccgacag taaccgcgac gctgagtttc
    26041 tgtttacctc aatgcagcaa gcctttgtga agctgcgtcg cggcgaaacc gggcaactgc
    26101 cgccaccgat tcaaaatatg gatcagttct ggtcaccgtc cgaacagtat ggcgtgcagc
    26161 aggcgctaag tatgtcgctg gtgggcgata aagcgaaagt gcgtcatggc ttgcagtcga
    26221 tcctgcgcga aaccgacgcc gatgagatta tggttaacgg gcagattttc gaccaccagg
    26281 cacgcctgca ttcgtttgag ctggcgatgg atgtgaagga agagttgttg gggtagtgtg
    26341 ttttaacgta ggaagcctta tccgagatgg caacgctgtc ctacgtaggc ctgataagcg
    26401 aagcgcatca ggcattgtgt aggcagcaga aatgtcggat aaggcacctc tgattactga
    26461 tacaccggca gcacattaaa gctcgataag atatgcacca gtgcgttgcc gacgccaaac
    26521 accagaatca gcacaatcat cggcttgccg ccccatacac ggaatttcgg gctaccaaag
    26581 cgtttacgcg atgcgcgggc cagcagcgcc ggaacaattg ccgcccagat ggtagccgct
    26641 aagccagcat aaccaatggc gtacaggaat ccgttaggaa acagcaggcc acccacaacc
    26701 ggcggggcaa aggtcagcaa cgctgttttc aaacggccca cagccgagtc gtcgaaacca
    26761 aacagatctg ccagatagtc aaacaggccc agcgttacgc caaggaacga actcgctacc
    26821 gcaaagtttg agaacacaac cagcagaagg tccaggctac ggctgttcag tacgccgctt
    26881 aacgcctgta ccagcacatc aatattaccg cccttctcgg cgatgccgat aaactccgga
    26941 cgcgggatgt tacccatcgt cgccagcaac cagatggtat acagcgccag cgccatcagc
    27001 gtaccgtaca ccaggcattt cacgatggtt ttcggatctt tgccgtaata cttcatcagg
    27061 ctcggtacgt taccgtgata accaaacgat gccagacaga acggcagcgt catcaacaga
    27121 tacggtgcat aagacgcatt gctttcggcg acgttgaaca atgtcgcagg ctgcacatgc
    27181 cccagcaggc taccgaaggt gaggaagaag gtaatgactt tcgcccccag cacaatcgct
    27241 gtcatgcgac tgacggcttt ggtgctcaac cacaccacaa acgccaccaa cagtgcaaaa
    27301 ccaaaacccg ctgcccgtgc cgggacgttt agcgacatct ctgcgaaggt gtgatgcaga
    27361 atcgaaccac tggcagaaat ataggcatag gtcaggatgt agagcacaaa ggcaatggaa
    27421 atgccgttga ccacgttcca gcctttgccg agcaggtctt tggtgatggt gtcaaagctc
    27481 gaaccgattc ggtaattcag gttagcttcc agaatcatca agccggaatg cagcatacag
    27541 aaccaggtga aaatcagcgc cgccattgac cagaagaacc acgccccgga catgaccact
    27601 ggcagagaaa acatccctgc gccaataatg gtgccgccga taatcaccac gccgccaagc
    27661 agcgacggtg acgtttgggt ggtggttagt gttgccatga gggcttctct ccagtgaaaa
    27721 atagtgcgac tgcgttgtta tgcattgcac tgtaccagta catgagtaca aaaaacagaa
    27781 aaaaagcccc gatgggtaaa atcggggctg tatatattat tttacagatt gtgttcgctg
    27841 ttcagcgatg attacgcatc accaccgaaa cgacgacgac cggtagagtc atcacgacgc
    27901 ggtgcgcggc cttcacgacg ttcgccgcta aaacgacgcc catcaccacg gccaccttca
    27961 cggcgttcac cgctgaagtt acgaccgcct tcacgacgtt cgccaccgaa accacgacca
    28021 ccgccacgac gctcaccgcc agtatgcggc tgtgcatcgc ccagtaactg catgttcatt
    28081 ggcttgttga gaatgcgagt acgcgtaaag tgttgcagca cttcacccgg catacctttc
    28141 ggcagttcga tggtggagtg agaagcaaac agcttgatgt taccgatgta acggctgctg
    28201 atgtcgcctt cgttagcaat cgcgccaacg atatgacgaa cttcaacacc atcatcgcgg
    28261 cccacttcaa tacggtacaa ctgcatatcg ccaacatcac gacgttcgcg acgcggacga
    28321 tcttcacggt cgccacgcgg gccacggtcg ttacgatcgc gcggaccacg gtcatcacgg
    28381 tcacggaatt cacgtttcgg acgcatcggc gcatctggcg gaacaatcag agtacgttca
    28441 ccctgtgcca ttttcagtag tgcggctgcc agagtttcga gatccagctc ttcaccttcg
    28501 gcagtcggct gaattttgct cagcagtgca cggtattgat ccagatcgct gctttccagc
    28561 tgctgctgta ctttagcggc aaatttttcc agacgacgtt tgcccagcag ttctgcgttc
    28621 ggcagttcta cttccggaat ggtcagcttc atggtgcgtt cgatgttgcg cagcagacga
    28681 cgctcacggt tctcaacgaa cagcagtgca cgaccagcac gacctgcacg accggtacga
    28741 ccgatacggt gaacgtaaga ctcagaatcc atcgggatat cgtagttaac caccaggctg
    28801 atacgctcaa cgtccaggcc acgggctgca acgtcggtcg caatcaggat gtccagacga
    28861 ccatctttca ggcgttccag tgtctgctca cgcagcgcct ggttcatgtc accgttcagc
    28921 gcggcgctgt tgtagccgtt acgctcaaga gcttctgcca cttccagagt cgcgtttttg
    28981 gtacgaacga agataatcgc cgcatcaaaa tcttccgctt ccaggaaacg taccagtgct
    29041 tcgtttttac gcatacccca gacagtccag tagctctggc tgatgtcagg acgggtagtc
    29101 acgctggact gaatgcgcac ttcctgcggc tctttcataa agcggcgggt aatgcgacga
    29161 atcgcttccg gcatggttgc agagaacaga gcggtctgat gaccttccgg gatctgcgcc
    29221 ataatggttt caacgtcttc gatgaagccc atgcgcagca tttcgtcagc ttcatccaga
    29281 accagaccgc tcagtttaga gaggtccaga gtgccacgtt tcaggtggtc cagcagacgg
    29341 cccggtgtac cgacaacgat ctgcggcccc tgacgcaggg cgcgtaattg cacgtcataa
    29401 cgttggccgc cgtacagggc aaccacgttt acgccgcgca tgtgtttaga gaaatccgtc
    29461 attgcttcag caacctgtac cgccagttcg cgggtcggtg ccagcaccag aatctgtggt
    29521 gctttcagct caggatcaag attctgcagc agaggtaaag agaatgctgc agtttttccg
    29581 ctccccgtct gggccatacc cagaacgtcg cggccattca gcagatgtgg aatacactct
    29641 gcctgaattg gagatggttt ttcgtaaccc agatcgttaa gggcttcaag gataggagcc
    29701 ttcaggccca gatctgcaaa agtggtttcg aattcagcca tgtagtacgt gtgcctcaaa
    29761 attaatggcg gccagtctac ataactcatc atgaaattga tcagcaattt tcattgaaaa
    29821 gtgtgaaccg gctcaaagta ggtgtattaa cgaacaacaa cgccctcacc cgttaaggtg
    29881 atggcaatca aaaaagatta cgggctgatg tgtacgtcag ctattgctgg tccgattctg
    29941 ccaggtcatc ttggtcctgg cccaggagcg ataattccaa caatgcgtat cggtgctcaa
    30001 caaagttatg aacgttgttg gcaaccgcca gtttgaacag tgccgtggcg ctgtccaaat
    30061 cccccagact taggtagtac ttacctaaat agaagttggt ttcactgaga tgctcagcga
    30121 gcgaggtgtt atccgttgcg tccgccttga gcctttccat taacgtttgt tcgctaatgt
    30181 tgcccaggta gaactcgaca atgttccatc cccactgttc cttatccgat ttttcgaagt
    30241 gctgtttcaa cacttcttta gcctgcttct catcgagctt ctgctcggcg agataaagcc
    30301 acagactacg gaaaggatca ttgggatcgt cttgataaaa cgccagcaga tcatcttgcg
    30361 ctaacttgtc acgaccgccg taatataatg cgatcccgcg attcaagtgc gcgtagttgt
    30421 aagttggatc aagctcaagt acagaatcaa acgcttcata ggcagcatca aaattgcctg
    30481 cctgcgttaa atatatgcct aagtaattga atacttcagg catatccggt cggattgcca
    30541 gcgcttgcga aaaatcgtta cgcgctaatg ccctcagacc gagactatca tacaacactc
    30601 cgcgctcata taaaagctgt gcgcgttcgt catcggttaa agcccgactg gcaaggattt
    30661 gttccatacg tgccagaatc acttcctgct gtaaagtcgg ttgcaatggt accgcgagga
    30721 cttcactttt acgccaggaa gtattactgc atcctgcaag cgtaagtgct gtcgcaacga
    30781 aacaccagcg caaaaaaggc ttcatttccc actcccgaag accacggttg aatgaacgtc
    30841 ctgttcccgg ttgctaacaa ggcgtcctgc ccggttaaaa gccccccgcc gcagcggagg
    30901 gcaaatggca accttactcg ccctgttcag cagccggagc ttccggtgct gcagcaggtt
    30961 gagactgctc agtcgcttct ttaatgctca gacggatacg gccctggcga tcaacttcca
    31021 gaactttcac cggtacttcc tgacccatct gcaggtaatc ggtcactttc tcaacgcgtt
    31081 tatcagcgat ttgagagatg tggaccagac cttctttacc gccgccgatg gcaacaaatg
    31141 cgccaaagtc aacgattcgg gtcactttac cattgtagac gcggcctact tcgatttctg
    31201 cagtgatctc ttcgatacga cgaatagcgt ttttcgcctt ctcgccgtcg gtcgctgcga
    31261 tcttcacagt accgtcatct tcgatttcga tggtagtgcc agtttcttcg gtcagagcac
    31321 ggattacaga accgccttta ccgataacgt ctttgatctt gtctggattg atcttgatgg
    31381 tatggatacg cggagcgaac tcagagatat caccgcgcgg cgcattgatc gcctgttcca
    31441 ttacgcccag gatatgcaga cgcgcacctt tagcctggtt cagtgcaacc tgcatgatct
    31501 ctttggtgat accttcaatt ttgatatcca tctgcaatgc agagataccg tcacgagaac
    31561 ccgcaacttt gaagtccata tcgcccaggt gatcttcgtc gcccaaaatg tcagacagta
    31621 caacgtagtt gtcgccttct ttcaccagac ccattgcgat acccgcaacg gcagctttga
    31681 tcggcacacc ggcgtccatc agcgccagag acgcgccgca cacggaagcc atagaagagg
    31741 aaccgttgga ttcggtgatt tcagacacaa cacgtacggt gtacgggaat ttgtccatat
    31801 ccggcattac tgccagcacg ccgcgcttcg ccagacgacc gtgaccaatt tcacgacgct
    31861 tcggagaacc gaccatgccg gtttcgccta cggagtacgg agggaagttg tagtggaaca
    31921 ggaaggtatc ggtacgttcg cccatcagtt catcaagaac ctgcgcgtca cgagcagtac
    31981 ccagcgttgc ggtaaccagc gcctgcgttt caccacgggt gaacagcgca gaaccgtgag
    32041 tacgcggcag cacaccagta cgcacatcca gaccacggat catatctttt tcacgaccgt
    32101 cgatacgcgg ttcgccggcc agtacgcggc tacgaacaac atttttctcg atagcgtgca
    32161 gaatttcacc cagttcgttt tcgtccaggg tttcgtcttc agcaagcagt gtcgcgatgg
    32221 tttcagattt gatgacatca acctgcgcat aacgctcttg tttgtcggtg atgcggtaag
    32281 catcgctcag acgagcttca gccagcgcag caacgcgcgc gttcagcgct tcgtttaccg
    32341 gctccggctg ccaatcccaa cgcggtttac cggcttcttt caccagttca ttgatgttct
    32401 gaataacaac ctgctgttgt tcatgaccga acaccactgc gcccagcatt tggtcttcgc
    32461 tcagcagttc agcttcagat tcaaccatca gcacggcagc ttctgtaccg gcaacaacca
    32521 ggtccagttt gctctctttc agttcgtcct gagtcgggtt cagtacgtac tggtcattga
    32581 tgtaacctac acgggcagca ccaatcgggc cattgaacgg aataccagac agagacagtg
    32641 ctgcggaagc accaatcatc gcgacgatat ccgggttaac ctgcgggtta acagaaacca
    32701 cggtggcgat aacctgaact tcgttgacga agccttccgg gaacagcggg cgaatcgggc
    32761 ggtcaatcag acgcgcgatc agggtttcgc cttcgcttgg gcggccttca cgacggaaga
    32821 agctacccgg gatacgacca gcagcgtagg tacgctcctg atagttaacg gtcagcggga
    32881 agaagtcctg acctggtttg gcttttttct gaccaacaac ggtaacaaat accgcggtgt
    32941 catccatgct aaccataaca gcggcagtag cctgacgagc catcatgccg gtttccagag
    33001 tcacggtgtg ttggccgtac tggaatttac gaacgatcgg attaagcaat gtaatatcct
    33061 ttctctttct tagacagtac cttacggtac tggtgttaat acccgatctt ctgcgcatcc
    33121 tcgcgactaa tgacaaccct aacccagctc tatgtgggta aagcctctca ttagccgcgc
    33181 gaacctctgc aacggaagat cattcatagc aacaatacat tagtttccag tgaattgctg
    33241 ccgtcagctt gaaaaaaggg gccactcagg cccccttttc tgaaactcgc aagaattagc
    33301 gacgcagacc caggcgctcg atgagctggg tgtaacgtgc tacgtcttta cgtttcaggt
    33361 agtcgagcag tttacgacgc tgagaaacca tgcgcagcag accacgacgg ctgtggtgat
    33421 cttttttgtg ctctgcaaag tggccctgca ggtggttgat ctgtgcagtc agcagtgcta
    33481 cctgaacttc ggtagaaccg gtgtcgtttg cgtcacgacc aaactcagaa acgattttag
    33541 ctgttgcttc agtacttaga gacattttaa aactccaaag tatatagaat gaaaggacgc
    33601 cgatctctaa ttcagcgatc ccagtgtacg ttacgcaaag tgttaaacaa tttacgcgac
    33661 gttaagcggc agtattctac tcgtagcgac ctgttatcgc aagacggtta acattacgcc
    33721 gggtattcaa ccaccaggcg acgaggcgca acgcggcctt catcgtcaat ttcgcccata
    33781 ccgataaact tgccgttctc accctctgta acgcgaacca gtccttccag cggcgcaccg
    33841 gatgtacgaa ctgggttacc atttttgaag taaacagaag acgttaacgg aagattcacc
    33901 actgggtagt ccgaagctgg actgtccatt ggcatcagta atgggtcaag taactccgca
    33961 gccggaatat cctgctgttc ggcttgctca accagttcac gcagatgttc cagggtcacc
    34021 atccgttcaa ccggatattt acttaccgcc agacgacgca agtaaataac atgcgcgcca
    34081 caaccgagtt tttcgccgag atcgtcaatg atagtgcgga tataagtgcc ttttgagcag
    34141 tgaatttcca gctccagctc attgccttca tggcgaataa acagcaattc ataaacggta
    34201 atcgggcgcg cttcacgtgg aacttcaatg ccctgacgcg cgtattcgta cagttttttg
    34261 ccctgatatt tcagtgctga atacatcgaa ggaatctgtt cgatatcgcc acggaaggta
    34321 tccagcgccg ccgccagttg ctctgcacta aaggttaccg gacgttcttc aacgatttgt
    34381 ccgtcggcat cagaagtatc ggtacgctgt ccaagacgcg caatgacccg ataacgtttg
    34441 tcggagtcca gcagatactg ggaaaacttc gtcgcttccc cgaggcaaat tggcaacatg
    34501 ccggtcgcca gcgggtccag tgcaccggta tgcccggcac ggttggcgtt ataaatccgt
    34561 ttcacttttt gcagcgcatc gttgctggac atcccctgcg gtttatccag caacaaaacg
    34621 ccgttaatgt cgcgaccgcg acgacgagga cgactcatta gtcctccttg ctgtcgtccg
    34681 ggttaacacg acgttcttcg tcatgtttga ccacgctggt caccaggttt gacatgcgca
    34741 tcccttcaac cagagagttg tcgtagaaga aggtcagttc cggcacgata cgcaggcgca
    34801 tcgctttccc cagcaggctg cggatgaaac cagaagcttc ctgcaacgct ttgatgcccg
    34861 ctttaaccgc gtcttcatct ttgtcgttga ggaacgtcac atatactttg gcatacgcca
    34921 ggtcgcgaga catttcgaca ccggaaacgg tggtcatcat gcccagacga ggatctttaa
    34981 tttcacgctg caggatgaga gcgatctctt tttgcatttc ctgcgctacg cgctgcgggc
    35041 gaccaaattc tttcgccata ataaattctc ctgacaaaaa aggggctgtt agcccctttt
    35101 taaaattaat ttcaggtgga agggctgttc acgttggcct gataagacgc gccagcgtcg
    35161 catcaggcaa tccatacagg atgcggcata aacgccttat cccgcatgga accctaaaaa
    35221 ccttaagcga tggtacgttg gatctcgatg atttcgaata cttcgatcac atcgccagtg
    35281 cggacatcgt tgtagttctt aacgccgata ccacattcca taccgttacg gacttcgtta
    35341 acgtcatctt tgaagcgtcg cagggactcc agctcgcctt cgtagataac cacgttgtcg
    35401 cgcagaacgc ggatcgggtt gtgacgttta accacacctt cggtaaccat acaacctgcg
    35461 atagcaccaa atttcggtga tttgaacacg tcacgaactt ccgccagacc gataatctgc
    35521 tgtttcagtt ccggagacag cataccgctc atcgccgctt tcacttcgtc gatcaggtta
    35581 tagatgacgg agtagtaacg cagatccagg ctttccgctt caatcacttt acgtgcagag
    35641 gcatcagcac gtacgttgaa gccaaccagg atggcgttgg acgccgcagc cagggtagcg
    35701 tcggtttcgg tgataccacc tacgccagaa ccgatgatct tcactttaac ttcgtcagta
    35761 gacagtttca gcaaggagtc agaaattgct tccacagaac cctgtacgtc tgccttcagg
    35821 acgatattca cttcgtgaac ttcgccttcg gtcatgttgg cgaacatgtt ctcgagttta
    35881 gatttctgct gacgcgccag tttaacttcg cggaatttac cctgacgata aagtgcaact
    35941 tcacgcgctt tcttctcgtc acgtacaacg gtaacttcat cacccgcagc cggtacgccg
    36001 gacaggccga ggatttccac cggaatggac ggacccgctt ccagcacttc ctgacccagt
    36061 tcgttacgca ttgcacgaac acgaccgtat tcgaagccac acagaacgat atcgcccttg
    36121 tgcagagtac cttcacgtac cagaacggta gcaaccggac cacgaccttt atcgaggaag
    36181 gattcgataa ccgcaccgct cgccatacct ttacgtaccg ctttcagctc cagaacttcc
    36241 gcctgcagca ggatagcatc cagcagttca tcaataccgg tacccgcttt tgcagatacg
    36301 tgtacgaact ggctttcgcc gccccactct tctggcagga tgccgtactg ggagagttcg
    36361 ttcttaacgc gatccggatc ggcttctggt ttatcgattt tgttcacagc aaccaccacc
    36421 ggaacacccg ccgctttcgc gtgctggata gcttcgatgg tctgcggcat tacaccgtcg
    36481 tctgcagcaa caaccaggac tacgatgtcc gttgcctgcg caccacgtgc acgcattgaa
    36541 gtaaacgcgg cgtgccccgg ggtgtccagg aaggtgatca tgccgttttc agtttcaacg
    36601 tggtatgcac caatgtgctg ggtaatgccg cccgcttcgc cagaggccac tttcgttgaa
    36661 cgaatgtagt ccagcagaga ggttttaccg tggtcaacgt gacccatgat ggtcacaacc
    36721 ggcgcgcgcg gttcagccgc agcacccgtg tcacggtcgc tcattaccgc ctcttccagc
    36781 tcgttttcac gacgcaggat aactttgtgg cccatctctt cagcaaccag ctgtgcggtt
    36841 tcctgatcga taacctggtt gatggttgcc attgcgccca gtttcatcat cgctttgatg
    36901 acctgagagc ctttaaccgc catcttgttc gccagttcgc caacggtgat ggtttcgccg
    36961 atcacaacgt cacggttaac ggcctgagct ggcttctgga agccttgctg cagcgaagaa
    37021 cctttacgtt ttccgccctt accgccacgt actgctgcac gagcttcttc acgatcagct
    37081 tttgattcag cgtgtttgtt gcctttcttc ggacgcgctg ctttcgcgtt acgaccacgg
    37141 ccacggccgc cttcgacttc acgatcgctt tcgtcttctg cctggcgagc atgttgagaa
    37201 gtagtgacgt gataatcgct ggaatcttca gtcggttccg cgttatcagt ccatttgttt
    37261 tcttccgcca tacgacgtgc ttcttcagca acgcgacgtg cttcttcttc gagtttacga
    37321 cgcgcttctt cttcggcttt acgcttgagc tctgcagctt cctgctcacg gcgggctttt
    37381 tcagcctggg cgtttttagt catatcgtct tgttgattgc tcactttgtc tttttccgca
    37441 gcttcacgtt tcgcttgttc agcagcttca cgcttagctt gttctgcggc ctcacgttca
    37501 gctttttgtt gcgcctcgcg tttagccgat tcttctgcct cacgacgggc ttgctcttcc
    37561 gcttcacgct gcgcttgctc ttccgctgca aggcgctcag cctcttgcgg atcgcgtttc
    37621 acaaaggtgc gtttcttgcg gacttcgatt tgtaccgatt tgctttttcc accggtacca
    37681 ggaatgttaa gggtgctgcg tgttttacgt tgcagcgtca atttgtccgg gcctgaattt
    37741 ttctgattca ggtggtcaat caaagtctgt ttctcttgtg cagacacaga gtcgtcagca
    37801 gacttccgga tacctgcatc agcaaattgc tgtaccaggc gttccacgga ggtctgtcgc
    37861 tctgcggcca gcgttttaat cgttacatct gtcatgctgt tccttcctgc tacagtttat
    37921 tacgcttcgt caccgaacca gcaaatatta cgggcagcca taatcagtgc tccggctttt
    37981 tcgtcggtca acccttcgat atcagccaga tcatcaatgc cctgttcggc gagatcttcc
    38041 agcgtacaaa cgccacgggc ggccagtttg aatgccaaat cacgatctac cccttcaagg
    38101 ttcagcagat catcagccgg tttgttatca ccgaggcttt cttcctgggc ctgtgcaatg
    38161 gtggccagtg catttttagc acgctcgcgc agtgcttcaa cggtcggctc atcaaggcct
    38221 tcgatttcca acagctcttt catcggcaca taggccagtt cttccagcgt cgagaagcct
    38281 tcttctacca gaacagtcgc gaagtcttca tcgatgtcga gatatttggt gaaggtgtcg
    38341 atcgctgcgt gcgcttccgc ctgatgctta gcctgcaggt cgtcaacggt catcacgttg
    38401 agttcccaac cgctcagctg cgaagccaga cgcacgttct gaccgttacg gccaatcgcc
    38461 tgcgccaggt taccggcttc aacggcgata tccatggtgt gtttatcttc atccaccacg
    38521 atagaagcaa cgtctgccgg tgccattgcg ttaatcacga actgcgccgg gttatcatcc
    38581 cacaggacga tatcgatacg ctctccgccc agttcagtag aaaccgcctg aacacgcgcg
    38641 ccacgcatac ctacgcaagc acctaccgga tcgatacgtt tatcgttggt tttcaccgca
    38701 attttcgcac gagatcccgg gtcgcgagcc gctgctttaa tttcaatcac ttcttcgccg
    38761 atttccggca cttcaatacg gaacagttcg atcagcattt ccggcttgga gcgggtgacg
    38821 aacagctgcg cgccacgcgc ttccgggcga acggaataga gcacgccacg aacgcggtcg
    38881 ccagggcgga agttttcacg cggcagcata tcttcgcgca ggatcacggc ttcagcgttg
    38941 ttgcccagat ccagagagat gttgtcgcgg tttacttttt tcaccacgcc ggtgatgatt
    39001 tcaccttcgt gttcacggaa ctgatcaacc accatcgcac gttcggcttc acgcactttc
    39061 tgcacgataa cctgttttgc cgtctgggta gtgatacgat caaaggtaac agactcaatc
    39121 tgatcttcaa cgtaatcgcc caagttcagg ctttcatctt cataacgtgc ggcttcaagg
    39181 gtgatttcct tggtcggctg ggtgacttca tcaacaacta accagcgacg gaaggtgtca
    39241 aaatcaccgc ttttgcgatc gatctgtacg cgaacgtcga tttcttgttc atattttttc
    39301 tttgttgctg tcgccagcgc gctttccaat gcttcgaaaa tcttctcgcg aggtagcgcc
    39361 ttttcattgg atacggcttc aactacagcc aaaatttctt tgttcatcgc gggcttttca
    39421 cctcatccag actattaaaa gtggggaacc aggttcgcct tctggatatt actcagcgcg
    39481 aacacttcat ctttaccttc gacggtaact gtgatcattt caccgtctac cgctttgata
    39541 acgccctgcc atttacgacg gttttgtacc gccatacgga gaaccagagt cacctcttct
    39601 ccgacaaaac gggcgtagtg ttcagccgtg aacagtgggc gatcgagacc cggtgaggag
    39661 acttccaggt tataagcaac ggtgatgggg tcttcaacat ccagcacagc acttacctgg
    39721 tggctcacat cagcacaatc atcaacattg atgccatctt cactatcaat atagatgcgc
    39781 agtgtggatg tgcgaccgcg aataaattcg atgccaacca gttcaaaacc cagggcctca
    39841 actggcgcag taatcatctc tgttaatttt tgctctaatg tggacaagcc cacccccaag
    39901 acataaaaaa agggcctaaa gcccagttat tctgtagtca gataacaaaa aaccccgata
    39961 aatcggggct ttatataact gaaccctata accgcaactg cggtctggag cactttccag
    40021 aaggattttt tcaaatccca ctacgaaggc cgaagtcttc acagtatatt tgaaaaagga
    40081 ctctaaggga aagtggttgc gggggccgga tttgaaccga cgaccttcgg gttatgagcc
    40141 cgacgagcta ccaggctgct ccaccccgcg cctgaaacgt ggcaaattct actcgttttg
    40201 ggtaaaaaat gcaaatactg ctgggatttg gtgtaccgag acgggacgta aaatctgcgg
    40261 gcattatagt gatccacgcc acattttgtc aacgtttatt gctaatcacg tgaatgaata
    40321 tccagttcac tttcatttgt tgaatacttt taccttctcc tgctttccct taagcgcatt
    40381 attttacaaa aaacacacta aactcttcct gtctccgata aaagatgatt aaatgaaaac
    40441 tcatttattt tgcataaaaa ttcagtgaga gcagaaatcc aggctcatca tcagttaatt
    40501 aagcagggtg ttattttatg acgacgattc tcaagcatct cccggtaggt caacgtattg
    40561 gtatcgcttt ttccggcggt ctggacacca gtgccgcact gctgtggatg cgacaaaagg
    40621 gagctgttcc ttatgcatat actgcaaacc tgggccagcc agacgaagag gattatgatg
    40681 cgatccctcg tcgtgccatg gaatacggcg cggagaacgc acgtctgatc gactgccgca
    40741 aacaactggt ggccgaaggt attgccgcta ttcagtgtgg cgcatttcat aacaccactg
    40801 gtggactgac ctatttcaac acgacgccgc tgggccgcgc cgtgaccggc accatgctgg
    40861 ttgctgctat gaaagaagat ggcgtgaata tctggggtga cggcagcacc tataaaggaa
    40921 acgatatcga acgtttctac cgttacggtc tgctgaccaa tgctgaactg cagatttaca
    40981 aaccgtggct tgatactgac tttattgatg aactgggcgg ccgtcatgag atgtctgaat
    41041 ttatgattgc ctgcggtttc gactacaaaa tgtctgtcga aaaagcttac tccacggact
    41101 ccaacatgct tggtgcaacg catgaagcga aggatctgga atacctcaac tccagcgtca
    41161 aaatcgtcaa cccaattatg ggcgtgaagt tttgggatga gagcgtgaaa atcccggcag
    41221 aagaagtcac agtacgcttt gagcaaggtc atccggtggc gctgaacggt aaaaccttta
    41281 gcgacgacgt agaaatgatg ctggaagcta accgcatcgg cggtcgtcac ggcctgggca
    41341 tgagcgacca gattgaaaac cgtatcatcg aagcaaaaag ccgcggcatt tatgaagccc
    41401 ctgggatggc actgctgcac attgcgtatg aacgtttgtt gaccggtatt cacaacgaag
    41461 acaccattga gcagtatcac gcgcatggtc gtcagttggg ccgtctgctg taccagggac
    41521 gttggtttga ctcccaggcg ctgatgctgc gggactctct gcaacgctgg gttgccagcc
    41581 agatcactgg tgaagttact ctggagctgc gccgcgggaa cgattattca atcctgaata
    41641 ccgtctcaga gaacctgacc tacaagccag agcgtctgac gatggaaaaa ggcgactcgg
    41701 tgttctcgcc agatgatcgt atcggtcaac tgaccatgcg taacctggat atcaccgata
    41761 cccgcgagaa acttttcggt tatgccaaaa ctggcctgct ctcctcctct gccgcttcag
    41821 gggtgccgca ggtggagaat ctggaaaata aaggccagta attcgacatc aaccctgccc
    41881 ttcaggggtg gggttacctt gattatcctc tgtaaactcc tttctgagca tcacataatc
    41941 gaaaacatca tggtttgcat ccacaacccg cgagtctcct ttatagtgtg caatcacttc
    42001 ctcaagcgtt tgcggctgtt ccggctttgt tacccccatc cacgcattaa taatgtaatt
    42061 attgtaagct gtcgaaaaga tgttgttttc cgttgtgcga tccacgccat ccccaagaac
    42121 ggggctatac cagataaaca tagggacatg atatgcctgc tggctagcct ccctgcctcc
    42181 atgaaaatag acgttcttct tcgtagggtc gcgttccagg ccgtggtcgg caaaatacat
    42241 gaccgaggcg cggcgatctt ttaataattc aaaaacctga cctagcaaac tatctgtgta
    42301 atgaatggag ttgtcatagc aggcatcctg atcgtcctgc ggttgaaaca cagcgctgga
    42361 ttgcggatag gcgctacaag ccggttcatg gcttccattt aaatgaagaa caatcagttt
    42421 cttttgctgc gtcttttgct gtaacgcttg cgataaatgc ggcaacaaca attcatcaaa
    42481 tcctctgaca tagactgttt ccatggcgcg catggcgata ctggtaactg ctgtaccatt
    42541 ctgccgaaaa gcggattgcg agcttagcca gaaagtctga aatcctgctt gattagccat
    42601 attaataatg ttgtccggat aattatgaat gtcatgactc aaaacagaat cagcagttaa
    42661 agaaagggga accgacagcg cagtgtaagg tgcaccgctt attgcttgat taaacagttt
    42721 gatctgcttt ctttgtgctt cgacttgcgg tgtcgtagag tgtgtatatc catacaaaga
    42781 catattgtcg acacgtacag actcccccac aatcaacacg taggtatcaa tacctgtatc
    42841 cctgactgat aattgaaaat acggcaccgt gtttgcaatt gagagtaatc tttgatgctc
    42901 tttcgctgct aaagcaaaat aattgagatt gaaaaacggc gtataggtag caaatcgcga
    42961 cgctagtata tatggactga acgcctcttt attttttgca tctttataag caaattgaca
    43021 agcggaaaat aaactgcccg aaatgacaat cagcaataat attcctgtca ctttttttgt
    43081 cggcaaggaa acatcatatt ttattattac tgccaaaaaa agaagggata aaaaggcaaa
    43141 agcacataga taagggatat acattcccag cattttgaca acttcatccg gatcactctg
    43201 cagcacacta atcgcaaatc catcattgaa agttgtacca aagatacacc atgaatagag
    43261 gctaatactc atatctgccg cagtcagtaa cacaaatggt gcggcaataa gcaggcgaag
    43321 cggaagggat ggtatacgtt taacaaccag gatggtcaga acgaagaaca gcaatcgctg
    43381 aaccatatgc gctccggaag aagcgactaa gttcgtaata ccaaaaataa taacacaaag
    43441 atacaacaac caatgagatt taaaactttt aacaaatttg ttgaatactg tcatccagaa
    43501 atccaagttc acaaccaata taaacaatta tcaacaatgt tcaattttat atcagacatg
    43561 tttttcaact ctaatccata tcagaccgaa acactgctag attttttctt tttaaatgat
    43621 gcggccattg cgttctcagt aattaaataa cattatcaat gcgtctctaa acattacgca
    43681 gaacgtagcc atcagtagtt atactgacag gggcaggatg gtttatcgag aagtaaacat
    43741 atccgtattc gtaccggaaa tggagttgac acaataaagt gccaattatg tcagcagaag
    43801 ggaaaagctt ataactaagg cataccggca ccatcgtttc aaggtaccag ctacgagtaa
    43861 aacaactgga cgagatacag atacctgaca accattcctc agaccaggac caaaacgaaa
    43921 aaagacgctt ttcagcgtct ttctggaatt tggtaccgag gacgggactt gaacccgtaa
    43981 gccctattgg gcactaccac ctcaaggtag cgtgtctacc aattccacca cctcggcacg
    44041 gatactactt tttagttcgg gatatcgctg gtcggcttag ccggagcagc tggctgagtt
    44101 tgttcggttt tcgccggcgc actcaggttt tcccattcgc tacctttatt ggttttgttg
    44161 ctattgatgt tacccagcac cagactgatg atgaagaata acgttgccag cagcgccgtc
    44221 atgcgagtca tgaagttacc agaaccactt gaaccaaaca gcgtagcgga agcgcctgct
    44281 ccgaaggagg ctcccatatc agcgccttta ccttgctgca gcatgatcag accaacaagg
    44341 ccaattgcca caataaggaa aactactaaa agagcttcgt acataatcaa cctgttcctt
    44401 gcggagttgc cgcgtaccaa tgcttcaacc aataaagcgg gaatatttaa cctttcccac
    44461 tgaagcgggt gtgaatacta accaaagcga atgaccttcg caagggcaat tttattgcat
    44521 tgtatcaact gcggaaaaaa acagcaaaac cgatgtgttc gctgagaaaa aaggcgacaa
    44581 tcgccgcctt tttagccagc catttatcgt tttaaacggc ttttactgca tcggcgatgc
    44641 ggtgtgcaaa ttcagtcacc tgcgcttcgt cttcgccttc caccatcacg cgaattaacg
    44701 gttcggtgcc ggatttacgc aacagcacac gtccacggct acccagcgcc gcttcaactt
    44761 ctgcggtcac agctttgact gattcatgct ccaacggatc gccgctacct gcggtgtaac
    44821 gcacattaac cagaatctgc gggaacattt tcatgccgct gcaaaggtcg tgcaggctca
    44881 tatggttacg cgccatcgcc gccagcacct gcaagccagc aacaatgccg tcaccggtag
    44941 tggttttatc cagcaggatc acatgaccgg agttctctgc accgatacgc cagcctttct
    45001 cctgcatttt ttccagtacg tagcggtcac ccactttcgc gcgcgcaaat ggaataccta
    45061 actgtttcag cgccagttca agccccatgt tgctcatcaa tgtacccaca gcaccaccac
    45121 gcagctggcc ctgacgaaga ccttcacgcg cgatgatata catgatctga tcgccatcga
    45181 ctttattgcc ttcatggtca accataatca cgcgatcgcc gtcgccgtcg aaggcaatac
    45241 cgagatccgc tttttcagcc agcacacgag cctggagcgc gcgaacgtcg gtagccccca
    45301 cttcggcatt gatatttaca ccgttcggct cacaaccgat agcgataacg ttcgccccca
    45361 gttcacgcag cacgttcggt gcgatgtgat aagtcgcacc gtttgcacaa tccaccacaa
    45421 tcttcagttc actgaggcta agttcgttcg ggaacgtggc tttgcaaaac tcgatataac
    45481 gacccgcggc atcaacgata cggctggctt tacccagttc tgccgaatca acacagctga
    45541 tctctttttc catttccgct tcgatggctt cttcaactgc atccggcagc ttagtaccgt
    45601 caatagaaaa gaatttaatg ccgttatcgt agaatgggtt atgcgatgca gaaattacaa
    45661 ttccagcctc tgcgcggaag gtacgcgtca gataagccac ggccggtgtt ggcatcgggc
    45721 cagtgaagag cgcggaaaga ccggcagccg caagacccgc ttccagtgct gactccagca
    45781 tatagccaga aatacgcgtg tctttaccaa taataatctt acgagagccg tggcgcgcca
    45841 gcactttacc cgcggcccaa cccagcttaa gcacaaaatc aggtgtgatc ggcgcatccc
    45901 ctacacgacc acgaatccca tcggtaccga aatatttacg attactcata gcgtttgttt
    45961 tcctttgcag acagagtggc ttccaccacc cgcatcgctt ctacggtttc tttgacgtca
    46021 tgaacacgaa tgatgtgcgc gccttgcatt gcggcaatga ccgcacaggc cagactaccg
    46081 ctcaggcgct cggacggccc cacgttcagc aattgcccaa tcatcgattt tcgtgacata
    46141 cccaccaaca gcggcaggtt gaaatggtga aattcagcca ggcgcgccag taatgaatag
    46201 ttatgggaga gatttttacc gaaaccgaat ccggggtcga gcaacaattt ctcttttgcg
    46261 atacccgcct gctcgcaacg tgctatttgc tcaataaagt agcgattcac ttctgcaaag
    46321 acatcgtcat acttcggcgc ttcctgcatg gtttttggat ttccctgcat atgcatcaga
    46381 caaaccggta aaccggtttc tgcagccgcc tccagagcac caggttcgga aagggagcgg
    46441 atatcgttaa taatgtgagc gccaactttc gctgactcac ggatgacttc tggtttggat
    46501 gtatctaccg agatccagac ttcgaagcgt tgagcaattg cctcaaccac aggaataaca
    46561 cgttgcaact cttcttcaac gcaaacttcc gccgcccctg ggcgcgtgga ctcgccacca
    46621 acgtcaatga tcgtcgcgcc agcgttgatc atcagattcg catgtttcac cgcatctaac
    46681 agcgagttat gcgtgccacc atccgaaaag gaatcaggcg tgacgttgag gatccccatt
    46741 acgtgaggat ggctaaggtc cagtgaagta ccctgggcaa agagtttcat gatgttatcc
    46801 ctggtatgaa ttgataagaa aaaaccccgg agcacgcccc ggggttttcg gtacaaatac
    46861 agtcatctga tacgggaact tacttgtcgc ctaactgctc tgacatggtg ttacccgggt
    46921 tcggcgtacg cggttcatca accggacgag gagcctttgg actaccattg tcgccagaat
    46981 tgttagaagc gcctggttct tcccagcccg ctggcggacg tacatcgcga cgtgccatca
    47041 ggtcatcaat ctgcggtgcg tcgatagtct catatttcat gagagcatct ttcatcgcat
    47101 gcagaatatc catattgtcg gtcagaagct gacgcgcacg attatagtta cgctcaatca
    47161 gtgctttcac ttcctggtcg atgatacgtg cagtttcatc ggacatatgt ttcgctttcg
    47221 ctacgctacg gccgaggaac acttcacctt cttcttccgc gtacagcagt ggacccaatt
    47281 tctcagagaa gccccactgg gtcaccatgt tacgtgccag gttggtcgca actttaatat
    47341 cgttggacgc cccggtagaa acatgttccg gtccgtagat gatctcttcc gccagacgac
    47401 caccgtacag cgtggagatc tgactttcca gtttctgacg gctggcgctg attgcgtcgc
    47461 cctcaggcaa gaagaaagtc acacccagcg cacgaccgcg tgggataatc gtcactttgt
    47521 gcaccggatc gtgttccggc accaggcgac cgataatcgc atgacccgcc tcgtggtaag
    47581 ccgtcgattc tttctgcgct tccgtcatca ccatggagcg acgttccgca cccatcatga
    47641 ttttgtcttt cgctttctcg aactcaacca tcgaaacaac gcgtttgttg ccacgagcag
    47701 cgaacagtgc cgcttcgttc accaggttcg ccaggtcagc accggagaaa ccaggagtac
    47761 cacgggcaat gattgccgcg tcgatatcgg gtgccaatgg tacgcgacgc atgtgaactt
    47821 tcaggatctg ctcacgaccg cgaacatctg gcaagccaac cacaacctga cggtcgaaac
    47881 ggccaggacg cagcagggct gggtcgagaa cgtccggacg gttagtcgca gcgataacga
    47941 tgataccttc gttaccttca aaaccatcca tctcaaccag catctggttc agagtctgtt
    48001 cacgttcatc gtgaccaccg cccaagcctg cgccgcgctg gcggcctacg gcgtcgattt
    48061 catcgataaa gatgatgcac ggtgccgctt tcttcgcctg ttcgaacatg tcacgaacac
    48121 gggatgcacc cacaccgacg aacatttcta cgaagtcaga accggagata gtaaagaacg
    48181 gaactttcgc ttcgcctgca attgctttcg ccagcaacgt tttaccggta cccggaggac
    48241 ctaccatcag gacgcctttc gggatcttac cgccgagttt ctggaagcgg ctcggctcac
    48301 gcagatactc aaccagttca gcaacttctt cttttgcttc gtcgcagccc gcaacgtcag
    48361 caaaggtggt tttgatctga tcttccgtca gcatgcgcgc tttgctctta ccaaacgaca
    48421 tggcaccttt gccaccgccg ccctgcattt gacgcatgaa gaagatccag acaccaatca
    48481 gcaacagcat cgggaaccag gagatgaaga tagaagccag caggcttggt tcttcaggcg
    48541 gttcaccgac aaccttgacg ttcttggtca acaggttatc cagtaatttc ggatcctgaa
    48601 ccggaatgta agtggtataa cggttactat ctttcttggt aacgttgatt tcacgtccgt
    48661 tgatacgcgc ttcacgaacc tggtcgttat tgacctcttg taggaaggta gagtaatcca
    48721 ccttacggcc attagactcg ctgggcccaa agctctggaa tactgacatc agcacaacgg
    48781 caatgaccag ccagagtatt aggtttttcg ccatgtcact caagggatta acctcttatt
    48841 acaactgtgt taaaaacagc gtcaggatac tctatatcca gcatctttca aactttcgtc
    48901 tgaaatctcc cggttagggt ttacgcccgg tcgctacaat atacacttcc cgcgaacgtg
    48961 cacgagaaga gtccggctta cgaactttga ccttcgtaaa cagggagcga atttccctta
    49021 gatactcatc gaaaccttcg ccctggaaca ccttcactac aaaactgcca cctggcgcta
    49081 atacatcacg acacatttct agcgccagtt ccaccagata catggcacgg gggatatcca
    49141 ccgccggtgt tccgctcatg tttggtgcca tatcggacat gacaacctgg actttgctgt
    49201 cgccaacgcg ctccagcagt gctttcatca ccagttcatc acgaaaatcg ccctgaagaa
    49261 agtccacacc aacgatagga tccataggta aaagatcgca agcgatgatg cggcctttgc
    49321 cgccaatttg ggtgaccaca tattgtgacc aaccacccgg agcagcacca aggtcgacaa
    49381 ccgtcattcc cggtttaaag agtttgtcac tttgctgtat ttcatcaagt ttaaaccagg
    49441 cacgggaacg taaccccttt ttctgtgcct gttgaacata tttatcgcta aagtgttcct
    49501 gaagccagcg gctggagctg gcagaacgct tcttacctgt catttaactt tcccatgggg
    49561 ataactcatc gtaaccaatt gcgtaaattt ttacgcgcct atttggtgat atatgggaga
    49621 tggcggtaga atgacccgtt ttcaatccca acgtaagcaa aaaatacgat gaatctgagt
    49681 actaaacaaa aacagcacct gaaaggtctg gcacatccgc tcaagccagt tgttctgctt
    49741 ggcagtaatg gtttgaccga aggggtgctg gccgagattg aacaagcgtt agagcaccat
    49801 gaactcatca aggtgaaaat cgccaccgaa gatcgcgaaa ctaaaacctt gatcgtggaa
    49861 gctatcgtgc gcgaaaccgg cgcctgtaat gtacaggtca tcggtaaaac gctggtgctt
    49921 tatcgcccaa ctaaagaacg taaaatctcg ctgccacgct aagattatcc taaagttaca
    49981 cacattcgct gtgtaaaacg aggggttttc cgcaggcagg agagcaaaat gccacgctct
    50041 gttcgttgat aaaaggccgc atagcggcct ttttcctttc tttacaatac atcaacatct
    50101 tgagtattgg gtaattctta caggtattcc accttaatta cttcaaattc tacttcgccg
    50161 cccggcgttt tgatgaccac aacatcatct tcttctttgc cgatcaggcc acgagcaata
    50221 ggcgagttta cagaaatcag gttttgttta aagtcagctt cgtcatcgcc aacgatgcga
    50281 taagtctgtt cttcgtcaga atcgagattc agcaccgtta cggtagcacc aaaaataacg
    50341 cgcccattgt tgggcatttt ggtgacatca atcacctgcg cgttcgacag cttggcttcg
    50401 atgtctttga tacggccttc gcagaagccc tgctgttcac gagctgcgtg gtattcggcg
    50461 ttttctttca ggtcgccatg ctcacgcgct tccgcgatag cagcaatgat ttcaggacgg
    50521 cgcacagatt tcagaaaatc cagctcttcg cgtaattttt cagcgccgcg taaggtcatc
    50581 ggaatagctt gcatttgtta tacctcttga atattcctga tggggcaagt cttcacccac
    50641 cccggctgtt cggcctgccc ggcataactc ctcaccggga ccagaagcaa aaaaataccg
    50701 acccgggtac aagtcccagg tcagctacaa ttcacatttt gatagtcatt ttaccctgga
    50761 gttcccgaag ggtcatcgtt tactttatag ggcgttgcgc cgtagtatga cggctcgatt
    50821 ccaggttgtt agcgcgagat tatgcgattt tccagattta tcatcggatt gaccagctgt
    50881 atagcgttca gtgttcaggc cgcaaatgtt gatgagtaca ttactcaact ccccgcaggt
    50941 gccaaccttg ccctgatggt gcaaaaagtc ggcgcgtcgg cccccgctat tgattaccac
    51001 agtcagcaga tggcgctacc cgccagtacg cagaaagtga ttactgcgct ggcggcgttg
    51061 attcaactcg gccccgattt tcgttttacc acgacgcttg aaaccaaagg caatgtggaa
    51121 aacggcgtac ttaagggtga cttagttgcg cgatttggtg ccgatccgac gttaaaacgt
    51181 caggatattc gcaatatggt cacgactttg aaaaaatctg gcgtcaacca aatcgatggc
    51241 aatgtgttga tagatacctc cattttcgcc agccacgata aagcccccgg ctggccatgg
    51301 aatgacatga cacaatgctt tagcgcaccg cctgccgccg ccatagttga ccgcaactgt
    51361 ttctccgtct cgctctacag tgccccaaaa cctggtgata tggcttttat acgcgtggca
    51421 tcttattacc ccgttacgat gttcagccag gtacgcaccc tcccccgtgg ttctgccgaa
    51481 gcgcaatact gcgaactgga tgtcgtacca ggtgacctga accgctttac gctaacggga
    51541 tgcctgccac aacgttctga gccgctcccg ttggcttttg ccgtgcagga tggtgccagc
    51601 tatgccgggg caattctgaa agatgagtta aaacaggcgg gtatcgcctg gagcggaaca
    51661 ctgctgcgcc agactcaggt taacgaacct ggaacggtag ttgccagtaa acagtcggcc
    51721 ccgctgcacg atctgcttaa gattatgctg aaaaagtcgg acaacatgat tgccgatacg
    51781 gttttccgca tgatcggcca tgcgcgcttc aatgtgcctg gaacatggcg ggccggatcg
    51841 gacgccgtgc gtcagatcct gcgccagcaa gccggtgtcg atatcggaaa caccattatt
    51901 gccgatggtt cagggctttc gcggcataac ctgattgccc ccgccaccat gatgcaggtg
    51961 ctgcaatata ttgcccaaca cgacaatgaa cttaacttta tctccatgct gccgctggcg
    52021 ggctatgacg gctctttgca gtaccgtgca ggtctgcatc aggcgggcgt ggatggaaaa
    52081 gtctcagcga aaaccggttc gttgcagggg gtatataacc tggcgggatt cattaccaca
    52141 gcgagcgggc aacgaatggc gtttgtgcaa tatctttctg gctatgcagt agaaccggcg
    52201 gatcagcgta atcgccgtat tccgttagtg cgttttgaaa gccgtttgta taaagatatt
    52261 tatcagaata attagcctaa agagaaaccc cggcacatgg ccggggtttt gctgattaac
    52321 gcttgtaaat gaactcaacg ccttcttcgt cgtcttcgtc ccagtcgtca tcccagtcct
    52381 catcatcctc ttcagcaatc tcttcaagct gctgacggtg ataatcatcc cacatgaatt
    52441 cgactttctc tggctgtttc gcttcttcag cctgcacgac cgggttttca atgataaagg
    52501 tcatcacatc ccagcagaga tctttcacgc ccagtccgct cgccgcagag atcagataat
    52561 atttatcttc ccagcccagc gcttcagcga tcgctttcgc tttctcttcg gcttctgcct
    52621 tatccagcag atcgatcttg ttgaaaacta accaacgcgg tttcgccgcc agatcctggc
    52681 tgtatttttc cagctcactg ataataatac gcgcgttttc aaccggatcg gtgccgtcaa
    52741 tcggatcgat atcgatgagg tgcaacagga cgcggcaacg ttccaggtgc ttcaggaagc
    52801 gaatgcccag acctgcgcct tctgcagcgc cttcaatcag ccctggaata tcggcaacaa
    52861 cgaagctctt ttcgttgtcc attcgcacca cgcccaggct tggtaccaga gtggtaaacg
    52921 gataatccgc cactttcggt ttagccgccg atacagcacg aataaaggtc gatttacccg
    52981 cgtttggcat ccccaacata ccgacgtcag ccagcagcat cagctccagc agcagctcac
    53041 gcttatcgcc cggcgtaccg ttagttttct gccgtggtgt acggttaact gacgatttga
    53101 aacgagtgtt gcccagaccg tgccagccgc ccttagcaac cagcagacgc tgaccgtgtt
    53161 tggtcatatc acccatggtt tcaccagtac cctggtcgat tacacgcgta cctaccggca
    53221 ctttaatcgt gacgtcttta ccgcgcttac ctgtacagtc gcggcttgca ccgttttgac
    53281 cgcgctctgc acggaaagat ttttcaaaac gataatcgat aagcgtgttc aggttctcgt
    53341 cggcttccat ccatacgtca ccaccgtcac cgccgtcgcc gccatccggg ccgcctttcg
    53401 gaatatactt ttcgcggcgg aagctcacgc aaccattacc gccatcacct gcaacgacca
    53461 gaatcgatgc ttcatcaaca aacttcattt tattctccgt aaatcattcg cctgcgcggg
    53521 gttgcgatac caccgttgta tgcttacgta atccgcccca aatacgatga ccaatggcgg
    53581 aatacatcgc gcccgcaacc acgacaaacg caccgagata acctaaaagg tttaacatcg
    53641 gtctggcgaa gaaatcgggc caggccagtg ataaaagatc tgaaaaaaac agcgtaaaca
    53701 gtggggtgag cgtgatgatc gcgctcacct gcgctgcctg ccagcgagcc atcgcttccg
    53761 ccagggcgcc atatcctacc aatgtattca gtccgcaaaa aattaaacag gcgagctgcc
    53821 agtggctaag ctgcgctatc actccaggat ttgccagagg gaagagcgca attgtacata
    53881 aagtgtacag caaaaacagg atctgcggtg aggccagccg acgcaataaa accttttgcg
    53941 ccacgccata actcacccaa accgtcgccg caccgacccc aaagataact ccccaggtgt
    54001 aatcggtgag ctttgtaaat atctcgacca gactggtgtt aaaaaacatc accaggccgc
    54061 tcaggagcat caatgcccct acaacctgag tactgcgcat tttctctttc aggataaata
    54121 cgctggcaac catcatgcca actggcgaga gttgcccaat cacctgcgaa gcggtcggac
    54181 tcaggtattg caaggatgag ctgaacagga tgaagttccc aaacagcccg gcggtcgcca
    54241 ctgctaacaa aatcaaccag cgtggcttac gaaacacgcg taatggcggc aaccgcttct
    54301 tcaccgcaag aatggcacca aggccaatac tcgccatcaa gaaacggtaa aacacgattg
    54361 tcggaggttc catcacctcc agcacctgct tcattgcgat tggcaacgcc ccccagcaaa
    54421 ttgctgtggt gagcgccaaa agaatgccaa tgcctgcctg ctgcttcata ccgtattccc
    54481 tgcaaagcgc atatgcggat taacgtcgca ccatgcgcat ttttaccggt tatcgaatgt
    54541 aaaaagcccc gcaacgtgtt gcggggcttt catccgttac cgggacgcga aaaacttatt
    54601 cagcttcgat gctgataaat ttacggtttt tcgggccttt aacttcgaat ttcactttac
    54661 cgtctgcttt agcaaacaga gtgtggtcac gaccgcaacc tacgttagcg ccagcgtgga
    54721 atttggtacc acgttgacga acgatgatgc tacccgccag aacggattca ccaccgaaac
    54781 gcttaacgcc caggcgttta gcttctgaat cgcgaccgtt acgtgtggag ccgccagcct
    54841 ttttatgtgc catttgaaat ctctcctcag gtcttaggcg ctgatgccag taattttcac
    54901 atcagtgaac cactgacgat ggccctgctg cttacgatag tgtttacgac gacgaaactt
    54961 aacgatttta actttctcgc cacgaccgtg agcaacaact tcagctttga ttacgccgcc
    55021 atcaacgaaa ggaacgccga ttttgacttc ttcaccgttt gcgatcatca gcacttcagc
    55081 gaactcaaca gtttcgccag ttgcgatgtc cagcttttcc aggcgaacgg tctgaccttc
    55141 gcttactcgg tgttgtttac caccactttg gaaaaccgcg tacatataaa actccgcttc
    55201 cgcgcacacc ttttcaatga ttcagagtgc gctataaata ttcacaatag ggcgcgaata
    55261 ttacgcaaaa cgcacgcctt tgacaagtgc tacagtcaat acacgaagaa aaaaaacaca
    55321 acttgtacgg taacgtttat ctgtgccatt ttttcagtac aatcacccta tattcctaac
    55381 cataaaccct aagttgcctt tgttcacagt aaggtaatcg gggcgaaaag cccggctttt
    55441 gcgatgaatt tagaaaaaat caatgagtta accgcgcaag atatggcggg tgttaatgcg
    55501 gcaatccttg agcagcttaa ttccgacgtc caactgatca atcagttagg ctattacatc
    55561 gtcagcggcg gcggtaaacg tattcgtccg atgattgctg tactggctgc acgagctgtt
    55621 ggctatgagg gaaatgcgca tgtcaccatt gctgccctga tcgagtttat ccacacggcg
    55681 actctgctac acgacgacgt tgtggatgaa tcagatatgc gcaggggtaa agctaccgcc
    55741 aacgccgcat ttggcaatgc cgccagcgtg ctggtaggcg attttattta tacccgcgcg
    55801 ttccagatga tgaccagcct cggttcactc aaagtgctgg aagtcatgtc agaagccgta
    55861 aacgtcatcg cagaaggtga agttctgcaa ctgatgaacg ttaacgatcc ggacatcact
    55921 gaagaaaact acatgcgcgt tatctacagc aagactgcgc gtctgtttga ggctgccgcg
    55981 cagtgttccg ggattctggc tggctgtacg ccggaggagg agaaaggcct acaagattat
    56041 gggcgctatc tcggcactgc tttccagttg atcgacgatt tactcgatta caatgccgat
    56101 ggcgaacagt taggtaaaaa cgtcggcgac gatctgaacg aaggtaaacc gacgctgccg
    56161 ctgctgcatg cgatgcatca tggcacacca gaacaggcac agatgattcg taccgccatc
    56221 gaacagggta acggtcgcca tcttctggaa ccggttctgg aagcaatgaa cgcttgtgga
    56281 tctcttgaat ggacgcgtca gcgtgccgag gaagaagcag acaaagccat cgcagcgtta
    56341 caggtgctcc cggacacccc gtggagagaa gcacttatcg gcctcgcgca catcgctgtt
    56401 caacgcgatc gttaatcccc tcccctcatc ccgcgcagtg cgcgggatga ttccagtaaa
    56461 ttccataaaa cacttattca gctctataac tcctgaaaaa cagggctgtg tatctttata
    56521 tattctgaat attcacactc tttacaggaa ctttttagag caataggcca tcaggagtat
    56581 agtgatgctc gacagaaaca gaagaagtat tctgaatgaa agcgactaat ttaaggagtg
    56641 aggaaagatg gaaagtaatt tcattgactg gcatcccgct gacatcattg caggtttgcg
    56701 caagaaggga acgtcaatgg cggcggagtc tcgcagaaat ggtttgagtt cctcaacgct
    56761 ggcgaacgca ttatcacgcc catggccgaa aggagagatg attattgcga aagccctggg
    56821 aactgacccc tgggttatct ggccatcacg ctaccatgat ccgcagaccc atgagtttat
    56881 cgacagaacg cagttgatgc gtagctacac taaaccgaaa aaatgagtgg tctggcggta
    56941 gccccgcgaa cggggctgcc agctctcaga caattactcg cctttcacac gctcaatatt
    57001 tgcacctaaa gcgcgtagtt tgtcttcaat gcgttcgtag ccacgatcga tgtgataaat
    57061 acgatcaacc accgtcgtcc cttccgcaat acagccagcc agcaccaggc ttgctgatgc
    57121 acgcagatcg gttgccataa cctgtgcgcc agaaagtttt tcaacaccgt gacaaataac
    57181 ggtattgctt tcgatttcgg cgtgcgcgcc catacggctc agctctggca catgcataaa
    57241 gcggttttca aagaccgttt cggtgatgaa tccggtccct tctgccacca ggttcaacag
    57301 cgtgaactgg gcctgcatat cggtcgggaa tgccggatgc ggcgcggtac gtacgttaac
    57361 agccttcgga cgtttgccat gcatatccag gctaatccag tcttcgccga cttcgatgtc
    57421 cgctccagcg tcacgcagtt tcgccagcac ggcgtccaga gtatctggct gcgcgttacg
    57481 gcagataatt ttgccgcgag agatcgccgc cgccaccagg aaagtaccgg tttcgatacg
    57541 atccggcagc acgcgataga caccgccgcc taaacgttcc acaccttcga tgacgatacg
    57601 atcggtgccc tgaccgctaa ttttcgcacc cagcgtaatc aggaagttcg cggtatcgac
    57661 gatttccggt tcacgcgctg cgttttcaat aatcgtggtg ccttccgcca gggttgcagc
    57721 acacatgatg gtcaccgttg cgccaacgct gactttatcc atcacgatat gtgcgccttt
    57781 cagacgacca tcgacggaag ctttaacgta accttcttcc agtttgatgg tcgcgcctaa
    57841 ttgttcgagg ccagaaatgt gtagatcaac cggacgtgcg ccgatcgtac aaccgccagg
    57901 cagtgaaact tgcccctgac caaagcgcgc taccagcggc cccagcgccc agatagaagc
    57961 acgcatggtt ttaaccagat cgtaaggtgc gcagaataca ttaacgtcgc gggcatcaat
    58021 atgcacagaa ccattacgtt ctactttcgc acccagctgg cttagcagct tcattgatgt
    58081 atcgacgtct tttagtttcg ggacgttctg gatctctacc ggttcttccg ccagtagtgc
    58141 ggcaaaaagg ataggcagag cagcattttt agcgccggaa attgtgactt cgccctggag
    58201 cttcgttggc ccctgaacac gaaatttatc catttagttt gttctcagtt aacaattcat
    58261 atccgctacc ggcgaatcgc ccatagctca aaagccgttc agtttgcgat cgcgcgccca
    58321 ctccgcaggg gtatacgctt tgatcgacac agcatgaatg cggttatccg caatatattc
    58381 catcagcggg ccatagaccg tctgctgttt tttaacccga ctcatgccgt caaacaactc
    58441 acccacggca ataacctgaa agtggctgcc atcgccggaa acgtggactt cctggaggga
    58501 gagagcgttc atcaacacgc tctgaatttc attattttcc atgggatctt caatcatcag
    58561 ttaataaatc agcgaaacat cttagagcaa agttgcgctg gcataaataa gcaaaaagcc
    58621 tcgctgataa atcagacaag gctcgacttg caggcaggtt tgccggacag gcggttaacg
    58681 ccatatccgg cctgaaaaaa tttaacgagg cagaacatca gcaggcaaat tatacaattt
    58741 cgccagggta tacactttgt cgtttacccc ctgaagcgtc acattgttgc cctgcttttt
    58801 cgccagatcg ataagatgga gcagcagtgc cagtccaccc gtatccacgc gagacacacg
    58861 gctaagatcg atgctggtaa tccccttcac cgcttcctca cgtatttccc aaagcggtag
    58921 caaaacgtcc tgatccagct ctccggataa cgccagcgtg tcacccgtct gcatccagct
    58981 cagtgactcg ctcattattt tttctcttcc agagtgattt tctgttgaga aatcgatttc
    59041 agttgcgcag tcaggccgtc gatacctttg gtacgcagca gcgttcccca ctcgttttgt
    59101 ttggtggtga tcatactgac gccttcagca atcatgtcgt aagcctgcca gttgcccgtc
    59161 tgggagtttt tacgccactg gaagtccaga cgcaccggcg ggcggccatt cgggtcaata
    59221 atggtaacgc gaatcggcac aatggtttta tcacccagcg gctgttctgg cgcaatctga
    59281 taggtttgac cgtgatacat cgccagcgcc tgaccgtaag cctgcttcag gtactcacgg
    59341 aaagcggcaa agtaagcatc acgttgagct ggggtcgcac tcttgtaata ctggcccagc
    59401 accagcgcac cggcgtattt cacctgtacg tatggcaaca gttcctgatc aacaatggta
    59461 cgcagataat ccgggttggc ccgaatttgc ggttgctcat tcttcaggcg atcgaacgtt
    59521 ttctgcgccg cctcgtccat cagcttatac ggattggtct ggtctgccgc ggttgccgca
    59581 ctcagcggtg caatcaccag caaagcgacc atcattaaac gtttaaacat gcgtcggttc
    59641 tcctgaaatt atttcgttgt acccacaggt tcagtggttt cattattacc tggcgcagca
    59701 gctggcgcat cgccactatt cttattgtca tcgcctttac taccgtaaag gaactgacca
    59761 atgagatctt ccagcaccat cgcagacttg gtgtcctgaa ttgtatcgcc atccttcagg
    59821 atagcagtcc ccagttccgg gtcttcaaaa ccgacgttta atgccagata ttgttccccc
    59881 agcaggccgg aagtacgaat gctcagcgaa ctggtatcag gaatgtggtt ataacgttgt
    59941 tcaatttcca gcgttacgcg cggcagatag gttttcgggt ccagcgtaat atccgccacc
    60001 cggcccacaa caacgccacc aatgctgacc ggagagcggg ctttcaggcc gccaatgtta
    60061 tcgaacgtcg cataaagcgt ataggtcggt tcagtacgta tagacgtcac gttcgctgcc
    60121 ttcaggcaaa caaacagcgc cgcaagcagt gctgctaata aaaagatacc cacccaaatt
    60181 tcattttttt tcgtttgcat gaactcaatt cccaaacatc aatgcggtca gcacaaaatc
    60241 cagccccaga acagccagag acgagtggac aacggtgcga gtggttgccc ggctaatccc
    60301 ggcagacgtc gggatggcgt cgtagccgtt aaacaacgaa atccacgtca ccgtgatggc
    60361 gaacaccacg ctcttaatca gacagttgac gagatccata cgccagtcga cggcattttg
    60421 cattgccgac cagaagaacc cactatcaat gcctttccaa ctgacgccga ccagcgatcc
    60481 gccccagatc cccacggcga cgaagataac cgtcaacagt ggtaatgaaa taactccagc
    60541 ccagaaacgg ggagaaataa cccgacgcag cggatccacc gccatcatct ccatactgga
    60601 gagttgctct gtagcgcgca tcaggccgat ttctgcggtt agcgccgaac cagcacgccc
    60661 ggcaaacaac aacgcggcaa ccaccggccc cagttcacgc agtagcgata acgccaccag
    60721 catacccaga ctggtttccg cactataagt ggtcagaacc agataacctt gcagccccaa
    60781 caccattccg atgaacacgc cagaaacaac aataatcagc atcgacagga cgccgacatt
    60841 atagagctgg cgcaccagca gcggtgcatg tttgcgaaat tccggtttgc cgaccagcgc
    60901 attgaataac attaacccgg cccgcccgaa cgttctcagg gttttaatcc ctttatgtcc
    60961 gagcgacgcc agcgcattta acagcatgag tggcttaact ccctggtaaa agatcagcgt
    61021 gataatcgcc ggcaggatag cggaacggaa caggcccgtc agctatcccg tccagaaact
    61081 gacgtacgcg cggatcagga ttcgcctgca acgcctgggc actaccatga gcgacaattt
    61141 ttttgtccgc caggatccag gcgtgatccg caatacttaa cacttccggc acatcgtgag
    61201 aaaccaccac acaagtcacg cccagcgcgc tgttcagctc agaaatcagc ttcaccagca
    61261 cgcccatggt aatgggatct tgcccaacaa agggctcatc aaacatgatg agatccggct
    61321 ccagcgcaat cgcacgtgcc agcgctgcac gccgcgccat cccaccggaa agttcagaag
    61381 gcataagttt agccgctcca cgcagcccca cggcctccag cttcatcatc accgtactat
    61441 gcaacaatgg cgcgggaagt tgggtatgtt cccgcagtgg ataggcgacg ttgtcaaata
    61501 cgttcatatc agtgaacaac gccccggact gaaataacat gctcatccgt ttgcgcactg
    61561 tatacaggcg cgaacgagac atcgccggaa tattctcacc atcgaaaaga atctcaccat
    61621 gatctggtgc gatttgcccg ccaatcagac ggagtagcgt cgttttaccg atgcccgatg
    61681 gccccatgat cgccgtgatc ttccctcgcg gcacggtcag ggaaatatta tcgaagatgc
    61741 agcgattgcc acgcgtaaaa ctgacatcgc gcatatcgac taaattcgcc acagactgct
    61801 ccataattca cccttcgtct tgcgttgatt ttctaagcat ggcgctcaat ttaaccttga
    61861 acccaacata tttacagaat attacctgcc gtggttagcg aaagctggca tttgttttac
    61921 tttttagccg cataaagtca aaattaagaa tctgttacgg ctttctgaaa atcttcagcg
    61981 gaccggcgag tatacctgaa gaaaggacgt tagatgcttt tagctacggc actgttaatt
    62041 gttggtttac ttttggtcgt ttacagtgcc gaccgcctgg tttttgccgc gtctattctt
    62101 tgccgaacct ttggcatccc gccgttgatc atcggcatga cggtggtcag tattggtaca
    62161 tcgttaccag aaatcatcgt ctcgcttgcc gcgtctttgc acgaacaacg cgatttagcc
    62221 gttggtacag ccctcggctc aaacattatc aatatattgc tgatcctcgg tctggctgcg
    62281 ctggttcgtc cttttaccgt ccattctgat gttctacgcc gtgaattacc cttaatgttg
    62341 ttggtcagcg tggtggccgg ttccgtactc tatgacggac aacttagtcg cagcgatggt
    62401 atctttctcc tctttctggc tgtgctatgg ctgctgttca ttgttaaact tgcacgtcag
    62461 gctgaacgtc aggggactga cagcctgacc agagagcagc ttgcagagct gccgcgtgaa
    62521 ggtggattgc ccgtcgcatt tttatggctc ggcatcgcac tgatcattat gccagtggcc
    62581 acgcggatgg tagttgataa cgccacggtg ctggcgaatt actttgccat cagcgagttg
    62641 acgatgggtc tgacggcaat tgctatcgga accagcctgc cggaactggc aaccgcaata
    62701 gcaggggttc gcaaaggtga aaacgacatt gcggtcggaa atatcattgg cgcaaacatt
    62761 tttaatattg tcatcgtgtt gggtttaccc gcgctgataa cgccaggaga gattgatcca
    62821 ctggcgtaca gtcgtgacta cagcgtgatg ttgctggtga gcattatttt tgcgttgctg
    62881 tgctggcggc gctccccgca accgggccgt ggtgtagggg tattattaac tggcggattt
    62941 atcgtatggc tggcgatgtt gtactggtta tcgccaatac tcgttgaata actggaaacg
    63001 cattatgtcg cacgtagagt tacaaccggg ttttgacttt cagcaagcag gtaaagaagt
    63061 cctggcgatt gaacgtgaat gcctggcgga gcttgatcaa tacatcaatc agaatttcac
    63121 gcttgcctgt gaaaagatgt tctggtgtaa agggaaagtt gtcgtcatgg ggatggggaa
    63181 atcggggcac atcgggcgca aaatggcagc aacatttgcc agcactggta caccttcatt
    63241 tttcgtccat cctggtgaag ccgcgcatgg tgatttaggc atggtcaccc cgcaggatgt
    63301 ggtgattgct atctctaact caggtgaatc cagcgaaatc acggccttaa ttccagtgct
    63361 taagcgtctt cacgtaccgt taatctgcat caccggtcgc ccggagagca gcatggcgcg
    63421 cgccgcagat gtgcatctgt gtgttaaagt agcgaaagaa gcctgtccgt tagggctggc
    63481 accgaccagc agcacaaccg ccacgctggt tatgggcgat gccctcgctg tcgcgctgtt
    63541 aaaagcacgc ggctttactg ctgaagattt tgcgctctca cacccaggcg gcgcactggg
    63601 tcgtaaactt ctgctgcgcg taaacgatat tatgcatacg ggcgatgaga tcccgcatgt
    63661 taagaaaacg gccagtctgc gtgacgcatt gctggaagtt acccgcaaaa atcttggtat
    63721 gactgtcatt tgcgatgaca atatgatgat tgaaggcatc tttaccgacg gtgatttacg
    63781 ccgtgtcttc gatatgggcg tggatgttcg tcagttaagt attgccgatg tgatgacgcc
    63841 ggggggaata cgcgtgcgcc caggcattct ggccgttgag gcactgaact taatgcagtc
    63901 ccgccatatc acctccgtga tggttgccga tggcgaccat ttactcggtg tgttacatat
    63961 gcatgattta ctgcgtgcag gcgtagtgta aagattcaag gataaacaac aatgagcaaa
    64021 gcaggtgcgt cgcttgcgac ctgttacggc cctgtcagcg ccgacgttat ggcaaaagca
    64081 gagaacattc gtctgctgat cctcgacgtt gacggcgtac tgtcagatgg cctgatttat
    64141 atgggcaata acggcgaaga gctgaaagcg ttcaatgttc gtgacggtta tggcattcgt
    64201 tgtgcgctca cctctgatat tgaagtcgct atcattaccg ggcgaaaggc taaactggta
    64261 gaagatcgtt gtgccacatt ggggatcact cacttgtatc aggggcagtc aaacaaactg
    64321 atcgccttta gcgatctgct ggaaaaactg gcgattgccc cggaaaatgt ggcttatgtc
    64381 ggcgatgatc tcatcgactg gccggtaatg gaaaaagtgg gtttaagcgt cgccgtggcc
    64441 gatgcgcatc cactgttgat cccgcgcgcc gattacgtaa cgcgcattgc tggcggtcgt
    64501 ggcgcagtgc gagaagtttg cgacttatta ctcctggcgc agggcaaact ggatgaagcc
    64561 aaagggcaat cgatatgagt aaagccagac gttgggttat cattgtgcta tcactggcgg
    64621 ttctggtgat gatcggcatt aatatggccg aaaaagacga taccgcccag gtggtcgtca
    64681 acaacaatga tcccacctat aaaagcgagc atacggacac gctcgtctat aacccagaag
    64741 gggcactaag ctatcgattg attgctcaac acgttgaata ttattccgat caggccgttt
    64801 cgtggtttac gcagccggta cttaccacgt ttgataagga taaaatcccg acatggtccg
    64861 taaaagcaga taaagccaag ctgaccaatg accggatgct ctatttatat ggacacgttg
    64921 aagtcaatgc actcgtgccg gactctcaac ttcgcagaat cacgacggat aacgcgcaga
    64981 tcaatctggt gacgcaggat gttacctctg aagacctcgt cacgttatac ggaacaacat
    65041 ttaactccag cggtctgaaa atgcgcggca acttacgcag caagaacgcc gagctgattg
    65101 aaaaggttag aacatcctat gaaattcaaa acaaacaaac tcagccttaa tcttgtgctt
    65161 gccagctcac ttctggccgc cagcattccg gcatttgccg taaccggaga cactgatcag
    65221 ccgatccaca ttgaatcgga ccagcaatct cttgatatgc aaggcaacgt ggttaccttt
    65281 accggtaatg ttatcgtcac ccagggcacc atcaaaatta atgccgacaa agtggtcgtt
    65341 acccgtccgg gcggcgaaca aggtaaagaa gtgattgacg gctacggtaa accggcaacg
    65401 ttctaccaga tgcaggacaa cggtaaaccc gttgaaggtc acgcttccca gatgcactac
    65461 gaactggcaa aagattttgt cgttctgacg ggtaatgctt atctgcagca ggtcgatagc
    65521 aacattaagg gcgataagat cacttacctg gtgaaagagc agaaaatgca ggctttcagc
    65581 gacaaaggca agcgcgtaac caccgttctg gtgccgtcgc agttgcagga caaaaacaat
    65641 aaaggccaga ccccggcaca gaagaagggt aattaattcg ttatggcaac attaactgca
    65701 aagaaccttg caaaagccta taaaggccgt cgcgtggtag aagacgtcag cctgaccgtc
    65761 aactccgggg aaattgtcgg tctgctgggg ccaaacggtg ccggtaagac caccactttc
    65821 tacatggttg ttggcattgt gccgcgcgat gcgggcaaca tcattattga tgatgacgat
    65881 atcagtctgc tgcctctgca tgcacgcgcg cgccgcggta tcggctatct gccgcaggaa
    65941 gcctccattt tccgtcgcct cagcgtttac gataacctga tggcggtact gcaaattcgt
    66001 gacgacttgt ctgctgaaca acgtgaagac cgtgcgaacg agctgatgga agagtttcac
    66061 attgagcacc tgcgtgacag catggggcag tcactctccg ggggtgaacg tcgccgtgta
    66121 gaaattgccc gcgcactggc tgcgaatccg aaatttattc tgctcgacga accgtttgcc
    66181 ggggttgacc cgatctcggt tatcgacatt aaacgcatca ttgagcacct gcgcgacagc
    66241 ggcctgggcg tactgatcac tgaccacaac gtgcgtgaaa cactggcggt ttgtgaacgc
    66301 gcttatatcg tcagtcaggg gcatttgatc gcccacggca cgcctacaga aatcttacaa
    66361 gacgaacacg ttaagcgtgt ataccttggg gaagacttca gactctgata gggtagaagt
    66421 ttgcgacgtt ttagcaggag agtacgattc tgaacatgaa gcaaggtttg caactcaggc
    66481 ttagccaaca actggcgatg acgccacagc tccaacaggc aattcgtctg ttgcagttgt
    66541 cgacgctgga acttcagcaa gagctacagc aggcgctgga gagcaatccg ctgcttgagc
    66601 aaatcgacac tcatgaagaa atcgacaccc gcgaaacgca agacagtgaa acactggaca
    66661 ccgccgacgc gctcgaacaa aaagagatgc cggaagagct gccgctcgat gccagttggg
    66721 acaccattta caccgctggt acaccatccg gcaccagcgg tgactacatt gatgacgagc
    66781 tgccggtcta tcagggcgaa acgacgcaga ccttacagga ttacctgatg tggcaggtcg
    66841 agttgacacc gttttccgac actgaccgcg cgattgctac ctctatcgtc gatgccgttg
    66901 atgacaccgg ttatctgact gtcccgctgg aagatattct cgaaagtatg ggcgatgaag
    66961 agattgacat cgacgaagtt gaagccgtcc ttaagcggat ccaacggttt gatccagtcg
    67021 gtgtggcggc aaaagatctg cgtgactgcc tgctgatcca actctcccaa ttcgataaga
    67081 ccacgccatg gctggaagag gccagactga tcattagcga tcatctcgat ctattagcca
    67141 atcacgactt ccgcacttta atgcgcgtca cgcgtctgaa agaagatgtg ctgaaagaag
    67201 ccgtcaatct gatccagtcg ctcgatccgc gccccgggca gtcgatccag actggcgaac
    67261 ctgagtatgt cattccagat gtgctggtgc gtaagcataa cggccactgg acggtagaac
    67321 tcaacagtga cagcattccg cgtctgcaaa tcaaccagca ctacgcctcg atgtgcaata
    67381 acgcgcgcaa tgatggtgat agccagttta tccgcagcaa tctgcaggat gccaaatggt
    67441 tgatcaagag tctggaaagc cgtaacgata cgctactgcg cgtgagtcgc tgtatcgttg
    67501 aacagcagca agccttcttt gagcaaggtg aagaatatat gaaaccgatg gtactggccg
    67561 atatcgccca ggctgtcgaa atgcatgaat cgacgatatc tcgcgtgacc acgcaaaaat
    67621 acctgcatag tccacgaggc atttttgaac tgaaatattt cttttccagt cacgtcaata
    67681 ctgaaggcgg gggtgaagcc tcttccacag cgattcgtgc gctggtgaag aaattaatcg
    67741 cggcggaaaa cccagcgaaa ccgttgagcg acagcaagtt aacctctttg ctgtcggaac
    67801 aaggtatcat ggtggcacgc cgcactgttg cgaagtaccg agagtcttta tccattccgc
    67861 cgtcaaacca gcgtaaacag ctcgtttgac ccaaccgata aggaagacac tatgcagctc
    67921 aacattaccg gaaataacgt cgagatcacc gaggcactgc gcgaatttgt tacagccaaa
    67981 tttgccaaac ttgagcaata ttttgaccga atcaaccagg tctatgttgt tctgaaagtg
    68041 gagaaagtca cccacacctc agatgcaaca ctgcatgtaa acggtggtga aattcatgcc
    68101 agcgcggaag gtcaggacat gtacgctgcc attgatggct taattgataa gctggcacgc
    68161 cagttgacca aacataaaga taaactgaaa caacactaat tgtccgggca attagcatgt
    68221 gcatggcggc ctgttgtgcg gcacaacggg ccatttgtac ggttaatgct ccgaacctgt
    68281 tccactgttt gagtgggcag gttcttaggt gaaattatga caaataatga tacaactcta
    68341 cagcttagca gtgttcttaa cagggaatgt acgcgaagcc gcgtccactg ccagagcaaa
    68401 aaacgcgccc tggaaatcat cagcgagctg gcggcgaaac aacttagcct ggcgcctcag
    68461 gtggtttttg aagctatcct gacgcgggaa aaaatgggca gtaccggtat cggcaatggt
    68521 attgccattc cgcatggcaa actggaagaa gatactctgc gtgccgttgg cgttttcgtt
    68581 cagctcgaaa cacctatagc tttcgatgcc atcgacaacc aaccggtgga cttacttttt
    68641 gccctgctgg tgccggcaga ccaaactaaa acgcacctgc atactctgtc gctggtggcg
    68701 aaacgtctgg cggacaaaac catctgccgc cgtttacgcg cagcccagag cgatgaagag
    68761 ctgtatcaaa tcattacgga taccgaaggt actccggatg aagcgtagtt attcggtaat
    68821 gtctctttta gacgttgtga ggagaaacag tacatggtac tgatgatcgt cagcggacgt
    68881 tcaggttcag gtaaatctgt cgccctgcgt gcgctggaag atatgggttt ttactgcgtg
    68941 gataaccttc ccgtagtgtt gttacccgat ctggctcgaa ctctggccga tcgagagatt
    69001 tctgccgccg tcagcattga tgttcgtaat atgccggagt caccagaaat attcgaacag
    69061 gcgatgagta acctgcctga cgctttctca ccgcaactac tgttcctgga tgccgaccgt
    69121 aataccttaa ttcgtcgtta cagtgacacg cgccgactgc atccgctttc cagcaaaaac
    69181 ctgtcgctgg aaagtgctat cgacaaagaa agcgatttgc tggagcctct gcgttcgcga
    69241 gcggatctga ttgtcgatac ctcagaaatg tccgttcacg agctggcaga aatgctgcgt
    69301 acccgtctgt tgggtaaacg tgaacgtgaa ctgaccatgg tctttgagtc tttcggcttc
    69361 aaacacggta tccctatcga tgcagattac gtctttgacg tgcgcttctt gccgaaccca
    69421 cactgggatc cgaaactgcg tccaatgaca ggtcttgata aacctgtcgc cgcgttcctc
    69481 gaccgccaca cagaagtaca caattttatc taccagacgc gaagctatct tgagctatgg
    69541 ttacctatgc tggaaaccaa caaccgtagc tacctgacgg tcgccattgg ttgtaccggc
    69601 gggaagcacc gttcggtgta tattgcagag caactggcag actacttccg ctcgcgcggt
    69661 aaaaacgtcc agtcacgcca tcggacgctg gaaaaacgta aaccatgacc gtcaagcaaa
    69721 ctgttgaaat tacaaacaag ctgggcatgc atgcccggcc tgcaatgaag ctgtttgaat
    69781 taatgcaggg ttttgacgct gaagtgctgt tacgtaatga cgaaggcacc gaggctgaag
    69841 ccaacagcgt tattgcgctg ctgatgttgg attctgccaa aggacggcag attgaagttg
    69901 aagcgaccgg tccacaggaa gaggaagcac tggccgccgt tatcgccctc tttaactctg
    69961 gttttgatga agattaatct tcatcacttt gacattcaaa tcccttcaag ctcccccggc
    70021 aataagaaaa gcgcgtactg aaatcgttat caatcaacgt cgattgtaca tttcgtgcgc
    70081 tttttacttt cagaagaacc ttaagaaaac cttaagaagc attgtttaga tttcgtttaa
    70141 gttaatcgac catactggag atcgtcagaa aatatttcca ggagatggca tgattcgctt
    70201 atctgaacaa agtcccctgg gcaccggaag acatcggaaa tgttatgcgc atccggaaga
    70261 tgcccaacgc tgtatcaaga ttgtctacca tcgtggcgat ggtggcgata aagagatccg
    70321 ccgtgagtta aagtactacg cgcatcttgg tcgccggtta aaagactgga gtggaatacc
    70381 gcgctatcac ggtaccgttg agacggattg cggaaccggt tatgtctacg atgtgattgc
    70441 cgatttcgac ggcaagcctt ccattacgct gaccgaattt gcagaacaat gccgttacga
    70501 agaagacatt gcacaactgc gccagttact gaaacagctg aagcgttatt tgcaggataa
    70561 ccgtatcgtg acgatgtcgc tgaagccgca gaatatcctc tgtcaccgca tcagtgagtc
    70621 agaggttatc cccgtcgtct gcgacaacat tggtgaaagc acattgattc cactggcgac
    70681 ctggtcgaag tggtgttgtc tgcgtaagca agagcggtta tggaaacgat ttatcgccca
    70741 accggcgctg gctattgccc tgcagaaaga tctgcaacct cgtgagagca aaacgctggc
    70801 cctcacttcg cgcgaagctt aatcaagctg gtgttgttgc ataaacggct caccgcctaa
    70861 ctgatacatc tgccgtaaaa tccacgcctg acggctacgc acatagcccg atggcgcgga
    70921 gactttgaaa cgaagtggat taggtaatac agctgccagt aatgcagctt ccgaccgggt
    70981 aagtttgctc gcgggtttgt ggaaataacg ttgtgccgca gcttcgacgc caaacacacc
    71041 gtcgccaaat tcggcgatat tcaggtaaac cgtcaggata cgctttttgc tccagactgt
    71101 ttctatcccc agcgttaatc cggcttccag cccttttcga acccagctac gcccatccca
    71161 taaaaagaga tttttggctg tctgctgaga aatcgttgaa gcaccgcgaa tacggttttc
    71221 attgcgctcg ttgtgcgcca gggcttgctc aatggaagcg acatcaaagc cccagtgctc
    71281 aggaaatttc tgatcttctg cggcaatcac cgccagtccc atccacggcg agatttgatc
    71341 catactgacc cagtcagaat gtgccacgta acgaaaattg ccatgcagcc aggcgctgac
    71401 ttgccgctcg accattaccg ctgagaaagg aacaggcgca acgctaaaca acgcgatgcc
    71461 cccgccccag aaaatggcga ggacaaccat taaccgcaga agaaaacggc gaacgaaact
    71521 aaacaccgtt aagcggcttt tactcattca gccagaacca gcacgcggga aaccagctta
    71581 tcaatgccgc tcgccgcttc tgcaatgttc tgcgccagca tatatgccgg ggtggtgaca
    71641 atcttattgt cttcatcaac cacgatatca tcgacaggac acggcacatg ctccgcgccc
    71701 atctcttcca gcacttctgc ggtatcgata tcagtaccga tggtcaaacg tagcgggaaa
    71761 tcgaaaattt tcggcagtat cgccggtgca atacacataa aaccaagcgg ttttccggcc
    71821 tgatgcatcg cttgtgccag ctcctttaat tcacggtcaa cggtgcattc gctaccaaga
    71881 ctggcaaaat tgcttaaatt cttcgccgcg ccaaaacccc ccggcacaat caacgcatcc
    71941 agttcagcgg catcggcctg ggccagagga cggatttcac cgcgcgttat tcgtgccgct
    72001 tcaatcagca cattgcgcgt ttccgtcatc gcttcaccag ttaaatggtt gataacatca
    72061 acctgctgct tatccggtgc aaagcaaacc gcctgcgcac cgctgcgtga aatagctaac
    72121 aacgtcaata ccgcttcatg aatttcagaa ccgtcataga cgccgcatcc gctcagaatt
    72181 acgccaattt tcttcattgt gatcttcctt tcgcaattga ctgaaacaca ttaatttttt
    72241 taataaaaat gctacgcatc acacatttaa ctgattcatg taacaaatca tttaagtttt
    72301 gctatcttaa ctgcgtgcgg cctgaaaaac agtgctgtgc ccttgtaact catcataata
    72361 atttacggcg cagccaagat ttccctggtg ttggcgcagt attcgcgcac cccggtctag
    72421 ccggggtcat tttttagtgg cttttgccac ccacgctttc agtacttcta cgtcgtgacg
    72481 ccactcttct ttcatctctt caatccattc accgacgtta tcttcccagg cgggaaggtc
    72541 aggagactga atttgctgac ccagctgttg cagatggcgt aaccccactg accccgccgc
    72601 acctttaatt ttatgccctt cctcaacaat gcctttttta tcctgcgccg tcagattcga
    72661 ctccagcacg ctgacatagc ccggcatcat tctctcaaac accgctaacc cgtcggtgat
    72721 cagcttcggt cctacaagtt cgagatactg ttccagcatg ggaatatcga gcaatgcttc
    72781 tgatttactg ttctcttctg tcgtcaccgt actctcctca tcatcctggg tatcccagaa
    72841 tttcttgatc atcgcggtta gcgccggaac agaaagcggc ttactcagca catcatccat
    72901 tccagcattg aggtactctt gcttgtcttt cagcacgtta gcggttaagg ccaccagcgg
    72961 cggtaaatcc tcgcgcggat aacgcttcgt cagttcacga gagatatcca gcccggtcat
    73021 atctggcaac tgaatatcca gcaataccag gtcgtattcg cccggtttaa acatctccag
    73081 cgccgccttg ccggtcatgg cgacatcaac gctgttacct aatttttcca gcacagaacg
    73141 cgcgacaatc acgttcagtt caatgtcttc caccagcagc acattcagcg ccggtaaagg
    73201 catatcgtct tcatcaaacg catcatcaac ctcttctgct accgacggtg cgtggatcgt
    73261 caacgtaaag gttgaacctt tgccctgttc gctggtaacc gtaatatcgc cgcccatatt
    73321 tttcgccaga cgacgagaaa cggccagacc aataccggtg ccggtggcag gtttaccgcc
    73381 atgactgtct ttcacctggt aatacatggc gaagatttta tccagctcat cctgcggaat
    73441 gccgatacca gagtcttcca cttcaaaatg cagcatatcg ccttcatcgt agcgcacgcg
    73501 cacggtaacc tggccttgct gagtgaattt gacggcgtta ctgatgaggt tccacaagat
    73561 ctgccgtaaa cgcgtcccgt cggtaatgac ctgatgcggt aatggcagcg tcggctccag
    73621 attaaagcgc aatccttttt gttgcgcctg caatgcggag agattttcca gatcggcaag
    73681 gaagctggtg aaatcaaccg gctgattatc aagctggacc ttgcgccgtt ccatcttatc
    73741 catgtcgata atatcgttga agatattccc cagcgtgaca gccgaaacat ggatggtttt
    73801 gagatatttt tcctgctcgg cggtgagttc agtatccagc agaatgcggc tcaggccgac
    73861 gataccgttc agcggtgtac gcaattcgtg actgatggtg gagataaacg tcgttttgtc
    73921 gcggctggcg cgttcaagcg catcctgata ccgcttacgc tcggtaatgt cgcgaccaaa
    73981 gcccatcaaa ccgtgacgtt tacccacgcg gtcgtagtac ggcactttac ggatttcaaa
    74041 gcaggctttg cgtccgtcgg gataatccag ccactgctca taggtcagtg acacattatg
    74101 acggaacact ttttcatcgg tttcaatgac tttcgcagcg gcttctggtg agtaaacatc
    74161 agcaggtttc aggtgaacca gttgtttttc gctttttccg gtcagcagct ccatcgcgcg
    74221 gttacagccg gaaaactctt tatcttcgtt acgataaaaa accaggtcgg gtgaagcgtc
    74281 aaggaaggaa cgtaagaatg aggattgctg ctcgagctga atttgtgtct cttcgcgctc
    74341 tttgatttca attttcagtt ggccgaaggt ttcctgcagt tctgcttccg ctttttcacg
    74401 aacggcgatt tcctgattta gctgggcaat attatctttt aactgaacgt tgaggctcaa
    74461 atcgcgctcg cgcatctcct ccagtttttg caccagtcgt gacagacgtt gtcgtgactc
    74521 ctccagttgc tcgacgacca ccgatagaaa gtagaccgcc cacggcgtaa tcagcaaacc
    74581 aaagaagata gaacgaataa catcaatgct ttcgacctga ccatgcagca ccatggttac
    74641 cgccatttgt accacaatgg caagaacgac gagggccagc gccagcaaca ttgagaagcg
    74701 caccagacct aacttcatca tcaggtcaac ataatactgc gccagcagac gaatttgctt
    74761 cattagggaa ttccttcacg acaacctgcc ccaataatac ccaattctga cagttacgtt
    74821 ataaattgtg tgagaaatgc ggaattcccc tcaatggcct acgccgttgg aggtagccac
    74881 ggacgtccca gcgccgatcc ctgtacgcca tgttcattca gataacgatc cagctcgacc
    74941 attcccgtcc atcgattttc gcaccacagc ggagcaagca gcgtcgggcg acgggcgctg
    75001 gcggaaatac ggtggtagat aacttccggt ggcgtatggc gaatcatttc tccggcagtg
    75061 agcgtgtaat cctccagttc aattccgttc aaacgccccg cttcccaggc tttcgccata
    75121 atgctgcctt tcactatatg cagcggatgc agctttatgc cgtccacgcc ggtttcaacc
    75181 actcgctcca gcgtttgcaa acattcagcc tgcccttcgc caggcaggcc aacaattaag
    75241 tgagagcaca ccttcagccc acgctcacgc gccagctgcg tcgtgcgctg atagcaggca
    75301 aaatcatgac cgcgatttat gcgatgcagt gttttgtcgt gggcggtttg tagccccagc
    75361 tccagccaca cttcgtagcc ctggtcctta tattcgcaaa gcagatccag caccgcatcc
    75421 ggcacgcagt ccgggcgggt accaacacac aaaccgacaa tattggcctg gctcaccgcc
    75481 tgctgataca tcgaacgcag cacctgaact tccgcaaagg tgctggtata cgcctgaaag
    75541 taggccagat agcgtttagc gcggttaact aaattcgcct ggtgcgccag ttgctcggca
    75601 atggaacgat gctgctgcgc ttcatcggca aacgaggcaa cattacagaa tgtgcagccg
    75661 ccacgcccga tggtaccgtc acggttaggg cagctaaaac cgccgtgcag cgtcagctta
    75721 tgcacctttt gcccataacg acgggtgaga tcaccaccaa acatattgac taatttctgt
    75781 aactgcataa tctgatagac cgcgccttga aaagaggcca aagcctgcca tttttagccc
    75841 aattcggcga tgacctggat caatcgtccc agcctgcttt tatcaactgc ataaccaatc
    75901 aaaattaccg aaatttcatg cacaatcaca taaatcactg ttgcttatct tgtgtcagat
    75961 ttttttatct tcttatggat tttaggcaaa aacagtagca tgaaacgtta ttaccaatta
    76021 aggcagtata aaatgctggc tttctcatca attcaaggca ggataagggt taacacgcct
    76081 ttatgacagt caggaattgg ctgtttctct aacgacaccc cttttagcct taaatagaaa
    76141 atccacttta atttcataca attaataaag aattttgcgc taaagcacat ttctgtacca
    76201 ataagcttgc catttgacct gtatcagctt tcccgataag ttggaaatcc gctggaagct
    76261 ttctggatga gcagcctgct catcatattt atgcagtaat tgagatcccc tcttcaccgt
    76321 attaaccgat gcgaaaagga caacaagggg gcgaatgcga ggtgcgcgta tgacacgcaa
    76381 accccgtcgc cacgctctta ctgtgcccgt gcgcagcggt tcggaagtgg ggttcccgca
    76441 gagcctgggg gaggttcacg atatgttgta cgataaatcc cttgagaggg ataactgtgg
    76501 tttcggcctg atcgcccaca tagaaggcga acctagccac aaggtagtgc gtactgcaat
    76561 acacgcactg gcccgcatgc agcaccgtgg cgcgattctc gccgatggta aaaccggtga
    76621 cggttgcggc ttgctgttac aaaaaccgga tcgttttttt cgcatcgttg cgcaggagcg
    76681 cggctggcgt ttagcaaaaa actacgccgt cgggatgctc ttcctgaata aagatcctga
    76741 actcgccgct gccgcacgcc gcatcgttga agaagagctg caacgcgaaa cgttgtcgat
    76801 tgtgggctgg cgtgatgtcc ccactaacga aggcgtgctg ggtgaaatcg ccctctcctc
    76861 tctgccacgc attgagcaaa tttttgtgaa cgccccggca ggctggcgtc cgcgcgatat
    76921 ggagcgccgt ctgtttatcg cccgccgccg cattgaaaag cgtctcgaag ccgacaaaga
    76981 cttctacgtc tgtagcctgt cgaatctggt caatatttat aaaggtctgt gtatgccggc
    77041 ggatctgccg cgcttctacc tggacctcgc ggacctgcgt ctggaatcgg ccatctgcct
    77101 gtttcaccag cgcttttcca ctaacaccgt accgcgctgg ccgctggcgc agccgttccg
    77161 ctacctcgcg cataacggtg aaatcaacac catcactggt aaccgccaat gggcgcgtgc
    77221 gcgtacctat aaattccaga cgccgctgat ccctgacctg cacgacgccg cgccgttcgt
    77281 caacgaaacc ggctctgact ccagttcgat ggataacatg ctggaactcc tgctggcagg
    77341 tgggatggat atcatccgtg ccatgcgtct gttagtgcca cctgcctggc agaacaaccc
    77401 ggatatggac ccggaactgc gtgccttctt tgactttaac tccatgcata tggagccgtg
    77461 ggatggcccg gcaggcatcg tgatgtctga cggtcgtttt gccgcctgta acctcgaccg
    77521 taacggtctg cgtccggcgc gctacgtcat caccaaagat aagctcatta cctgcgcctc
    77581 tgaagtcggt atctgggatt atcagcctga cgaagtggtc gaaaaaggcc gcgtggggcc
    77641 aggcgaactg atggttatcg acacccgcag tgggcgtatt ctgcactcgg cagaaaccga
    77701 tgacgatctg aaaagccgcc acccttataa agagtggatg gagaaaaacg tccgccgtct
    77761 ggtaccgttt gaagatctgc ccgatgaaga agtgggtagc cgcgaactgg acgacgacac
    77821 gcttgccagc taccagaaac aatttaacta cagcgcggaa gagctggatt cggtgatccg
    77881 cgtactgggt gaaaacggtc aggaagcggt cggttcgatg ggcgacgata ccccattcgc
    77941 cgtgctctcc agccagccgc gcattattta cgactacttc cgccagcagt ttgctcaggt
    78001 aaccaacccg ccaatcgacc cgctgcgcga agcgcatgtt atgtcgctcg ccaccagtat
    78061 cggtcgtgaa atgaacgtct tctgtgaagc agaaggccag gcgcaccgtt taagctttaa
    78121 atcgccgatt ctgctctatt ccgatttcaa acagctcacg acgatgaaag aggatcacta
    78181 ccgcgcagat acgctggata tcacctttga cgtcactaaa accacgctcg aagcgacggt
    78241 gaaagaactg tgtgacaaag ccgaaaaaat ggtacgtagc ggcaccgtgc tgctggtgct
    78301 ctccgaccgg aatatcgcta aagatcgcct gccggttccg gctccgatgg cggttggcgc
    78361 gatccagacc cgtctggtcg agcaaagcct gcgttgcgat gccaacatca tcgtagaaac
    78421 cgccagcgcc cgtgatccgc accacttcgc cgtgttgctg ggtttcggcg cgacggctat
    78481 ttatccatac cttgcctatg aaacgctggg ccgcctggta gacacccacg cgattgccaa
    78541 agattatcgt accgtgatgc tcaactatcg taacggcatc aacaaaggct tgtacaaaat
    78601 catgtccaaa atgggcatct ccaccatcgc ctcttaccgc tgctcgaaac tgtttgaagc
    78661 ggtcggtctg cacgatgatg tcgtgggcct gtgcttccag ggggcagtta gccgcattgg
    78721 gggggcgagc tttgaagact tccagcagga tctgctgaat ctgtcgaaac gagcctggct
    78781 ggcgcgtaag cctatcagcc agggcggcct gctgaaatat gtacacggtg gcgaatacca
    78841 cgcctacaac ccggacgtgg tgcgcacgct gcaacaggcg gtacagagcg gcgagtatag
    78901 cgactatcag gaatacgcga agctggttaa tgagcgtccg gcaaccacgc tacgcgatct
    78961 gctggcaatt acgccgggtg aaaacgcggt caacattgct gatgttgaac cggcaagcga
    79021 actgtttaaa cgcttcgata ccgccgcgat gtctatcggt gcgttaagcc cggaagccca
    79081 cgaggcgctg gcggaagcga tgaacagcat cggcggtaac tcaaactccg gcgaaggcgg
    79141 tgaagacccg gcgcgctatg gcactaacaa agtgtcgcgc atcaagcagg tagcgtccgg
    79201 tcgctttggg gttactccgg cgtatctggt caatgccgac gtcattcaga taaaagtcgc
    79261 ccagggcgcg aagccgggtg aaggtggtca gttaccgggt gataaagtca ctccttacat
    79321 tgccaaactg cgctattcgg tgccgggcgt gacgctgatc tccccgccgc cgcaccacga
    79381 tatctactct attgaggatt tagcgcagct gattttcgac ctcaagcagg tcaatccaaa
    79441 agcgatgatc tccgtgaagc tggtttccga accgggcgta ggcaccatcg cgactggcgt
    79501 ggcaaaagct tatgccgatt taatcaccat cgcaggctat gacggcggca ccggcgcaag
    79561 tccgctttca tcggtgaaat acgcaggctg tccgtgggag ctggggcttg ttgaaaccca
    79621 acaggcgctg gttgccaacg gcttgcgtca caagatccgt ttgcaggtcg atggcggcct
    79681 gaaaacgggt gtcgatatca tcaaagcggc gattcttggc gcggaaagtt ttggcttcgg
    79741 cactggcccg atggtagcgc ttggctgtaa atatctgcga atttgccacc tgaacaactg
    79801 cgccacgggt gtagcaactc aggatgacaa actgcgtaag aatcactatc acggcctgcc
    79861 gttcaaagtg actaattact ttgagtttat cgcccgtgaa acccgcgagc tgatggcaca
    79921 gctgggggta acgcgtctgg tggatctgat tggtcgcacc gacctgctga aagagctgga
    79981 cggtttcacc gccaaacagc agaaactggc actgtcgaag ctgctggaga ctgccgagcc
    80041 gcatcctggt aaagcgctct actgcaccga aaacaacccg ccgtttgata acggcctgct
    80101 gaacgcgcag ttgctgcaac aggcaaaacc gtttgtcgat gaacgccaga gcaaaacctt
    80161 ctggttcgat atccgcaaca ccgaccgttc tgtcggcgcg tcgctttcag gctatatcgc
    80221 ccagacgcac ggcgatcagg ggctggcagc cgatcctatc aaagcgtact tcaacggcac
    80281 tgcaggccag agcttcggcg tgtggaacgc gggcggcgtg gaactgtacc tgaccggtga
    80341 tgccaacgac tatattggta aaggcatggc gggcggctta atcgccattc gtcctccggt
    80401 tggttccgcc ttccgcagcc atgaagcgag cattatcggc aacacctgcc tgtatggcgc
    80461 gaccggtggt cgtctgtatg ccgcaggccg cgcgggtgaa cgtttcggcg tgcgtaactc
    80521 cggtgctatc accgtggtag aaggcattgg cgacaacggc tgtgaatata tgacgggtgg
    80581 tatcgtctgt attctgggta aaaccggcgt taacttcggc gcgggcatga ctggcggctt
    80641 cgcttacgtt ctcgatgaaa gcggcgattt ccgcaaacgc gttaacccgg agctggtcga
    80701 ggtcttaagc gttgacgatc tggcgatcca tgaagagcat ctgcgtggtc ttatcaccga
    80761 gcatgttcag cataccggtt cccagcgtgg tgaagagatt ctggcgaact ggtcaacctt
    80821 cgccactaaa tttgcgctgg ttaaaccgaa gtccagtgat gtaaaagcac tgctgggtca
    80881 ccgtagtcgt agcgcagcag agttgcgcgt gcaggcgcag taaggggtag caacaatgag
    80941 tcagaatgtt tatcaattta tcgacctgca gcgcgttgat ccgccaaaga aaccgctgaa
    81001 gatccgcaaa attgagtttg ttgaaattta cgagccgttt tccgaaggcc aggccaaagc
    81061 gcaggctgac cgctgcctgt cgtgcggcaa cccatactgc gagtggaaat gcccggtaca
    81121 caactacatc ccaaactggc tgaagctcgc caatgagggg cgtatttttg aagcggcgga
    81181 actgtcgcac cagacaaaca ccctgccgga agtttgcgga cgtgtctgcc cgcaagaccg
    81241 tctgtgcgaa ggttcctgca ccctgaacga tgagttcggt gcggtgacca tcggcaacat
    81301 tgagcgttat atcaatgata aagcgttcga gatgggctgg cgtccggata tgtccggcgt
    81361 gaaacagacc ggtaaaaaag tggcgattat cggcgcaggt ccggcgggtc tggcgtgcgc
    81421 ggatgtcctg acgcgcaacg gcgtaaaagc gatcgtcttc gaccgtcacc cagaaattgg
    81481 cggcttgctg accttcggta ttccggcttt caagctggaa aaagaggtaa tgacgcgccg
    81541 ccgtgaaatc ttcaccggca tgggtattga attcaaactc aataccgaag tgggccgcga
    81601 cgtgcagctg gacgatctgc tgagtgatta cgatgccgtg ttccttggcg tcgggactta
    81661 tcagtcaatg cgcggcgggc tggaaaacga agacgccgat ggcgtgtacg cagcgctgcc
    81721 gttccttatc gccaacacca aacagttaat gggctttggt gaaacccgcg acgaaccgtt
    81781 cgtcagcatg gaaggcaaac gcgtggtggt ccttggcggt ggcgacactg cgatggactg
    81841 cgtgcgcacg tctgtacgcc agggggcaaa gcacgttacc tgtgcctatc gtcgtgatga
    81901 agagaacatg ccgggttccc gccgcgaagt gaaaaacgcg cgggaagaag gtgttgagtt
    81961 caaattcaac gtccagccgc tgggtataga agtgaatggc aacggcaaag tcagcggcgt
    82021 aaaaatggtg cgcacagaaa tgggcgaacc ggatgccaaa ggccgtcgcc gcgcggagat
    82081 tgtggcaggt tccgaacata tcgttccggc agatgcggtg atcatggcgt ttggtttccg
    82141 tccacacaac atggaatggc tggcaaaaca cagcgtcgag ctggattcgc aggggcgcat
    82201 catcgccccg gaaggcagcg acaacgcttt ccagaccagc aacccgaaaa tctttgctgg
    82261 cggcgatatc gtccgtggtt ccgatctggt cgttaccgcc atcgccgaag gtcgcaaagc
    82321 ggcagacggc attatgaact ggctggaagt ttaagcgagg taacaatgaa ttcgccagac
    82381 agtgtctggc gaatttataa aacgagctat ccaactaaat tttaagccat taaatcagcc
    82441 ttaaccttca ctaccacctt tttaatctca ccaggttcgc caacaacgca tcccggttta
    82501 tgcggttctc ctggcataaa cacggcgaac attcccggtt tcaggatgat ggcttgctcg
    82561 ttttcaatgg cgctgcaaag ctgataatca tcttcatggt ggaactcttc acactgacgc
    82621 gcagtgcctg ccatgccaaa cagaatccgt tcctcaccgt ttaataacag ctggatatca
    82681 atgtattgtt cgtgcaattc ggcttttttc tcaacgggcg attgagtatt aaatgtcatg
    82741 acattcataa agatattgtc gccctgtaat tcgtaacggc caggcgcttt ttcttgcggt
    82801 ctggcagcta atgccaacgt taacgcgtcc tgtaacgcag gatgtaatcc ggcagacggt
    82861 aatgactgta cttcacccat catcataata tttctccctg ggccaacagc gcagccccaa
    82921 gtaaacctgc atcgtggcgg tagtgcgccg ccagtaaatc aacatgaaat gccgctggct
    82981 cctgcgccag atacgtttcc accagcgcca gatacccttc tgccagacca acgctgccac
    83041 cgaccaccac gcactggcaa tcagttgtgg ctttaatatc agcgatcagc cttgcaagcg
    83101 tacgtgcgga gcggtgaatc agttgctgcg cctgctcatc gccctgcccg gcgcgcgtga
    83161 aaatagtttt cgcattcgca ccagccaact ccccctgcgc tgcagctgca atgccgcgac
    83221 cagaagcaat cgcttccacg caacctgtgc gtccacagcc gcagactgga ccatgcggat
    83281 cggcaagcgt atgcccgata tgccccgcca gaccgccagg gccggtacgc agtttaccgc
    83341 cgctcactac accgccgcca acgccggtgg aaacagtgat aaacaccata tcggttatat
    83401 cgccatccag cgcctgatac tccgcccatg ctgcggcctg cgcatcatta atggcaatgg
    83461 tcggcaaatt ggtaagttgc tccagcgttt tgactaacgg aaagtgtaac aatccaccga
    83521 gattatgcgg attcagcgcc agcaagctgc cgtcgcgaat aattccggtt gaagcgatag
    83581 caacccgctg cgcatgtgct tgcaacggag agactaatgc ggataaggca tcacgcagcg
    83641 cctgcggagt ctggctggca ggcgtaggaa gttctcggcg atcgcggatc tgcccgtcag
    83701 cgccaatcag cgcggcggca agtttagtac cgccgatatc aatcgccagt gtggtcatag
    83761 caccgccttt ttcatcgctg tgttgtacca ctgacaaatg tgttcaagac gcgtaatggc
    83821 agaaccgacc gtcaccgccc acgcgccgtg gcgcatcgca tccgccgcct gagcaggcgt
    83881 gttgtaacgc ccttcggcaa tcacccggca tccggcgtca ctcaacgttt tcaccagcgc
    83941 cagatccggc tcttctggcg tttcaggcgt ggtatagcca gaaagcgtag tgccaataat
    84001 ttcggctccc agcttttggc atgccaggcc gtcttccggc gttgagcagt cggtcatcgc
    84061 cagtaaaccg tgatggtgaa tacgtgccag cagcgtttca acaggcaccg gacgcggtcg
    84121 gtcagtgccg tcaatggcga taatgtccgc gcccgcctgc gccagcgcat caacatcttc
    84181 aatataggcc gtgatgcgta ccggagaatc ctccagatcg cgcttcacaa tcccaataat
    84241 cggcacgctc accaccgcac gcgtggcttg cagatttgcc acaccttcaa tacgaatggc
    84301 aaccgcgccc gcctgctctg ccgctaatgc catggcagcg acgatttcgg gtttatcgag
    84361 cgggctgtcc ggaaccggct ggcaggagac aatcaggcca ccgttagcag cgattttttg
    84421 atccagttgt gcaagtaacg acatacatct tcccttagcg aaaggcccgg tacagagacc
    84481 gggcaacagg attaactttt ggttttgact aaatcgtttt tggcgctgcc aaatggcacg
    84541 gcaccgctaa atggtttgcc gtcgatagcg tcatgagtac gcaatgcttc cgggcgcaac
    84601 caacgctgaa cacgagaagg catatccaga ccaatcagca ggatcaccac aaacgttaaa
    84661 ctaaacgaga gcgagccaag tgccgttccc agatccagac gttgagcgat caacgcgccg
    84721 aggattggag ctagtgcacc gcccagtgcg ccaacgttgt aggtaaagcc cagccccgcc
    84781 gcacgctggt cggtatcgaa ataaccgccg atcagtttcg gtaaaatccc tgagatgccc
    84841 tgaccaagca tttgctggaa gaacagtaac aaaccgagca cccagacgtt tgcgccgcca
    84901 atcgcaaata ccggaataat cagcagctgc gaggccagca gactacaaac gtacgctttg
    84961 cgggttccca gccagtcacc gaggaagcca ccgacgcagc accccaccgc cgcgccaaag
    85021 ccactaaaga acagcacatt ggcgacagta tgcgggtcat aagccagatc ggttttcaga
    85081 tacgttggca gcagcgcctg aatcggccat gagtagagga aagcgaacaa cacgaccacc
    85141 atcagcatta cgcccgttgg ccagcgtttg cccgtactct gcaccataaa gctgataaag
    85201 attgcggcgc ataacagccc gagtacagcg acgattgcgg cattttgcag gttaccggcg
    85261 aagcagaacc acagcgcagt agccgccgcc agcgtcatta cgatattggc aatgcgatgt
    85321 tcaccacggt aaagaatgtc taccatcgtg cgtactggtg ctttaccccc gtgtttctct
    85381 ttccagtctt ccgcttccgg gatgttttta cgcagccaga gagcaaagat gattggcaaa
    85441 atgccgataa agaacagcgc acgccagccc cagaccggaa ccaccagact atagacctga
    85501 gcggcaacga cggcccccac tgagaagcct gaaatcagaa aaccactggc tttgttacgc
    85561 agatgctttg gccagctttc aatgacatag gtggcgctgg aaccgtattc acccgccatc
    85621 cccatgccga tgaccagacg agcgataaac atggtgatgt agcctggcgc aaagccgcag
    85681 gccagcgtcc cggccgagaa gagaacgatg ctggtgacca ttgccagacg acgcccgtag
    85741 cggtcaccca tagcgccgag catcaggccg ccgaaccagc gagagataaa ggctgcggag
    85801 atcagacttg ccgcctgcac cgtcgtcagc ccgaattcac cctgtacttc ggtgagtacc
    85861 agggcgatta aaacgaaatc aaaaccgtca agcagatatc ccaaccaggc agcggaaaat
    85921 gcccgccatt gtgcgcggtt aagatggcga taccacggga tattctgggt tgtagtactc
    85981 attgtgagtc tcccgcggtg ggcgatgccc acacgctttg gtatgaaaat tgtagggtac
    86041 agatgcgttt atttcccctc acccggtagg ggcgagcgag gggaaacaac tcacccgcgc
    86101 tcttgcatca actgctgagc cagcgccttc agttctggca gatatttttc atctaccgga
    86161 ccaaacggtt tgcggcacag cggcacagaa acgacatcca tataatgcag gacagttttc
    86221 agcccacgga atacgccagt tttgatcagt aaatcaatga ctttgttgca ctcagtttgc
    86281 agtttctgcg cggtctggat atcgccttct ttcagcgcct tcacgatccc ctgataacgc
    86341 cagcccatga tgttgtaagt gctgccgata ccgccgtctg cgcccgccag aagaccagag
    86401 gcgaagattt cgtcgtaacc gttatagagc accagatcag gatgttcacg acggatctgc
    86461 tccatctgat agagatcgcc agaggtctgt ttcagcgcac ctacgccagg cagcgtaacc
    86521 aaggtgttga tctgatccag ggtcagtttt accccgctca gggctggaat gttgtacacc
    86581 accatcggca acccatccgc tgaatcaata attgcccgat agtgatcgca gtgttcttca
    86641 aagctgaaag gatagtagaa cggcgtgacg gcggagacgg catcgaagcc ataacgttta
    86701 gccgatgccg caagttgttg gctttcggcg gtgctgacgc aaccgacatg ggcgatgagt
    86761 ttaatcttac ctttcgcctc ttcagcgacg atttccagta cctgttcgcg ctcggaaagg
    86821 ctttgtacaa aggcttcgcc ggtcgaacca cccacgtaca aaccgtcgat gccctgctga
    86881 atattgaact gaaccaggcg gcgcagactc gctttatcca gtgcttgttg ttggtcaaaa
    86941 ggagtcagga gtgcagccat tacgccacgt aaattcgttg ccataaatac ctctgaagtg
    87001 atgcttgtct gataaacgat atacctttat acctgttata ccagatcaat taagcaacac
    87061 cccatacaga aagcttataa tgcgatctgc ttcactaaag tggcattatt tctttttgtt
    87121 ggtggtctga ccgaaggcgt gccaggtagc agagacgctg ttgagatgcg attgcaacgc
    87181 gcgatcggct tcgtcaggat catgacggcg gatcgcgtca acgatcgcaa tatgctgttg
    87241 atagctaacg ttgttatgct catgcagagc ctgatcggtc acggttggac gtgcggcgat
    87301 tagccagtcg agcagggcaa cgtggatcgc catgaagatt gggttacccg ggatctccgc
    87361 cagaacgcgg tggaaatcaa cgtctgagcg aatgaatgcc gcgttgttat ccagcgactg
    87421 actgttgatt tccagtgctt ttgccagcaa atcgatttgc tcatcggtgg cgtgttcagc
    87481 cgcatagcgc accaggctgg attcaaagaa cagacgtaat tgttcgaaat gggcaatccc
    87541 accaggatga gaaaggaaat ctttcgccat gccggaaagc tcgccgatga tggtgtccgc
    87601 agaaggacgt gagacgcggg cacgttcgcc gttgtttatt tgcaccagac ctttgcgttt
    87661 taacgctgcc agcgcttcgc gcaccgaagg acgcccgacg ttaaagaacg ccatcagttc
    87721 gcgttcagac ggtaattgtt caccttcgcc aaattcacga cggcggatca tctgttccag
    87781 ctcttcttcc accatttcgg agagtttttt acgcgccagc gggcggctgc gcaagttgcg
    87841 cccgatagtg gtagtagaat cttcagcttg cggatcaaat gcgttcatag gactcattca
    87901 ggaaaatgcg ttagcggata ataacgacgc tatcgtagca cagtatcaaa gtcaggcgaa
    87961 aagatgacag atagcaaaac aggccacatg ggcctgtttt cattttactt cacaacgcgt
    88021 aatgccggtc gaccaccgcg tggtggctgc ggaggttcat cgtcaggatg agtttcatca
    88081 tcgtgatctg gcttgtcgcc atcgataacc gacataacgg tttcgttgtc tgccgatgcc
    88141 tcttcatcat tcatgatgct ggcatcttca tcgtaggcag cttcaggctc aaacatcgtg
    88201 cctgcgccat tttcacgggc gtagatagcc agcacggcag ccagcggcac agaaacctga
    88261 cgcggaatgc caccaaagcg cgcgttaaag cgcacctcat cattcgccag ttccagattg
    88321 ccgacagcac gcggcgcaat gttgagtacg atttgcccgt cacgcgcata ttccatagga
    88381 acctgcacgc cagggagcgt cacatccacc accaggtgcg gcgtgagctg gttatccagc
    88441 aaccactcat agaatgcacg cagcagatag ggacgacgtg gtgttagctg tgacaaatcc
    88501 atacagatta actccggccc agacgcattt cacgttctgc ttcagttaaa gaagcaagga
    88561 aagagtcacg ttcaaagacg cgggtcatat agcctttcag ctctttcgca cccgggccgc
    88621 tgaactcgat gcccagttgc ggcagacgcc acagcagagg agcaaggtag caatcgacca
    88681 ggctgaactc atcgctcagg aagtacggct tctgaccgaa gaccggcgca atcgccagca
    88741 gttcttcgcg cagttgctta cgtgcggcat cggcttcaga agctgaaccg ttgatgatgg
    88801 tgttcatcag cgtgtaccag tctttttcga tgcgatgcat gtacagacgg ctttcaccgc
    88861 gagctaccgg gtaaacaggc atcagtggcg gatgcgggaa acgctcatcc agatattcca
    88921 taatgatgcg agattcccac agggtcagct cacgatccac cagggtcgga acgctctgat
    88981 tcgggttgag gtcaatcaga tcctgaggcg gattgtcctt ttccacgtgt tcgatctcga
    89041 aacttacacc tttctcagcc agcacaatgc ggacctgatg gctatagatg tcagtaggac
    89101 cggaaaacag cgtcattacc gaacgtttgt tggcagcgac agccatgaaa acctccaggt
    89161 atagtcagaa tttttactgc taccagccac caggtggcca gtcagaagtt gtgttaccca
    89221 gtaaggaacg actctctttg ttcgaaaatc aaacaaaaaa tgagcaatac ccgacatttg
    89281 ggcagaaaat tggatgatag tttaccagat tttgcgacct ttgtggtgag tcgatgccgg
    89341 aaatggggaa aaagagatgc gctttagtct gaaatagttg acttagtccc ttattggcga
    89401 tgtggttttt gttttacctg tctgtcaggt ggcagcaaaa agcaactttc cattttttac
    89461 gctgatgcag attttagcca taaaaaaacc cgccgaagcg ggttttttcg caaattgttt
    89521 tctgccggag cagaagccaa ttaacgtttg gagaactgcg gacgacgacg tgctttacgc
    89581 agaccgactt tcttacgttc aacctgacga gcgtcacgag taacgaagcc agctttacgc
    89641 agttcagaac gcagggactc gtcgtattcc atcagagcgc gggtgatacc gtgacggatc
    89701 gcaccagcct gaccagagat accaccacct ttaacggtga tgtacaggtc cagtttctca
    89761 accatgtcga ccagttccag cggctgacga actaccatgc gagcagtttc acgaccgaag
    89821 tactgttcca gagaacgttg gttgattacg attttaccgt tgcccggttt gatgaaaacg
    89881 cgagctgcgg aacttttgcg gcgaccagtg ccgtagtatt gattttcagc cattgcctat
    89941 aatcccgatt agatgtcaag aacttgcggt tgctgtgccg cgtggttgtg ctcgttaccc
    90001 gcgtaaactt tcagtttacg gaacatagca cgacccagcg ggccttttgg caacatgcct
    90061 ttaaccgcga tttcaatcac acgctcagga cggcgagcaa tcatctcttc aaaggtcgct
    90121 tgtttgatac caccgatgtg gccggtgtgg tgatagtaca ctttgtcagt acgcttgttg
    90181 ccggttacag caactttgtc agcgttcaga acgatgatgt aatcaccggt atctacgtgc
    90241 ggagtgtatt ccgctttgtg cttaccgcgc aggcgacgag ccagttcagt agccagacgg
    90301 cccagagttt taccggtcgc gtcaacaaca taccagtcgc gttttacggt ttctggttta
    90361 gctgtaaaag ttttcattaa aagcttaccc aataaatagt tacacgttgg tgaacaccca
    90421 aacgtcttca attgttgagg ttcacacgac aaagtccggc aaacctaccc cttcgaatag
    90481 cctatgccag cacacaaaaa gtttcgggaa aaaaaacttt cttgtaacgt ggggtcgcag
    90541 gattatagag aagtcggggt caaagatcga cccctttttg tgatttgtgg caggttttac
    90601 cccgccaaat gctcgcgctt caaatactct tcgctttgca tctcttgcag acgtgacagg
    90661 caacgctgga actcaaactt cagccgctcg ccctgataaa tttcatacag cggcacttct
    90721 gcactcacca ctaatttgac atggcgctcg taaaactcat ccaccagcgc aataaagcgc
    90781 cgcgcttcgc tctccatcaa ccgcgtcata actggtacat caaacaacat gaccgtgtga
    90841 aagaggcgtg agagcgcaat atagtcatgc tgactgcggg cgtcgacgca cagcgtagta
    90901 aaagaaaccg ccagcgtctg gttctcgacg cccattgtcg ccaatggccg atggttgatt
    90961 tctaacgtcg gtgaattttc tcgtttcgcc cccgccagcg ccaaccatag tttatccatt
    91021 tgcgcccggg tttcatcgtt gaggggcgaa agccacagat gcgcctgagt gagtgtacgc
    91081 aggcgataat caacaccagc gtccacgttc attacatcac aatgctgttt aatggcatcg
    91141 attgcaggca aaaaacgcgc acgttgcagg ccatttcgat aaagttcgtc cggcggaata
    91201 tttgacgtcg ctaccagggt aataccgcgg gcgaacaggg ctttcatcag accgccaagt
    91261 agcatggcat cggtaatatc agaaacaaaa aattcgtcaa aacagagcac gtcagtttct
    91321 gctttaaagc gatcggcaat aatttccagc ggatcggtct gcccctgcaa agcagttagc
    91381 tcttcatgca cccgcagcat aaaacggtga aagtgcaggc gctgtttccg ctctcccggc
    91441 agactttgat agaaaaggtc catcagccag gttttcccgc gtcctacacc gccccacata
    91501 tataagccac gcactggcat atgctttgtg tcttcgcgtt taccccacag cttaccgacc
    91561 cgcgccatta gcccactcgt cctgggggct ggtggcgtgc tattgacgag ttccttataa
    91621 ataatttcca ggcggctgac ggcctctttt tgaacgtcgt cgggttgatg gctgccttca
    91681 ttgagcgcct tcaggtattg cgatgttggg gtaacgcttt gcatgatctt attgttattc
    91741 cttgaataat cggtgcgccg ttgttcacgg ttgacgaaaa aaaggccgtt ctacactacg
    91801 cgatatgctg tcgggattcc acttctgtgg aattaacggt tatagtggca taatcagccg
    91861 caggcatgga gcctgaagcc aacaccctac ggaaacaaaa gacaacggga gatgttcatg
    91921 acctgggaat atgcgctaat tgggttagtc gtcggcatca ttattggtgc tgtggccatg
    91981 cgttttggta atcgtaaact acgccagcaa caggcgttgc agtacgaact ggaaaagaat
    92041 aaagctgaac tggacgagta tcgcgaagag ctggttagcc actttgcccg cagcgcggaa
    92101 ttactggata ccatggcgca cgactatcgc cagctgtatc agcacatggc gaaaagctcc
    92161 agtagcctgc tgccggaact gtctgcggaa gcaaacccgt tccgtaatcg tctggcagag
    92221 tctgaagcca gcaacgatca ggcaccggtg cagatgcctc gcgactattc tgaaggcgca
    92281 tccggcctgc tgcgtactgg cgcgaagcgc gactaattta tttttggggc gcagccattg
    92341 cgccctcctc ttctctccct ccccgactat catttaatct ggtgtctcat tgttagccgt
    92401 ctgaaaattc aataacatca aactgttttg aatctctttt cttatcattc aggtacgaga
    92461 gcaggaataa tgaaaaaaca aacccagctg ttgagtgcat tagcgttaag tgtcgggtta
    92521 actctctcgg cgtcatttca ggccgtcgcg tcgattccag gccaggttgc cgatcaggcc
    92581 cccctcccca gtctggctcc aatgctggaa aaagtgcttc cggcagtggt gagcgtacgg
    92641 gtggaaggaa cggccagtca gggacagaaa atcccggaag aattcaaaaa gttttttggt
    92701 gatgatttac cggatcaacc tgcacaaccc ttcgaaggtt taggctccgg tgtcatcatc
    92761 aacgccaata aaggctatgt gctgaccaac aaccatgtga ttaatcaggc acagaaaatc
    92821 agtattcagc tcaatgatgg gcgcgagttt gatgcaaaac tgattggtag cgatgaccag
    92881 agcgatatcg ccctgttgca aattcaaaac ccgagcaaat taacgcaaat cgctattgcc
    92941 gactccgata aattgcgcgt cggtgatttt gccgtggcgg tcggtaaccc atttggcctt
    93001 gggcaaaccg ctacctccgg gattatatct gcgttaggcc gcagcggtct gaatctcgaa
    93061 ggtctggaaa actttattca aactgatgcc tccattaacc gcggcaattc cggtggtgcg
    93121 ctgttaaacc ttaacggtga gttaattggc atcaacactg caatccttgc gcctggcggc
    93181 ggtagcgtcg ggattggatt tgccattccc agtaatatgg cgcgaacact ggcgcagcag
    93241 cttatcgact ttggagaaat caaacgcggc ctgttaggta tcaaaggcac cgagatgagt
    93301 gccgatatcg ccaaagcctt caaccttgac gtgcagcgtg gcgcgtttgt cagcgaagtg
    93361 ttgccaggtt ctggctcggc gaaagcgggc gtcaaagcgg gcgatattat taccagcctc
    93421 aacggcaaac cgctgaatag ctttgctgag ttgcgctctc gtatcgcgac caccgagccg
    93481 ggcacgaaag tgaagcttgg cctgctgcgt aacggcaaac cactggaagt agaagtgacg
    93541 ctcgatacca gcacctcttc gtcggccagc gctgaaatga tcacgccagc gctggaaggt
    93601 gcaacgttga gcgatggtca gctaaaagat ggtggcaaag gtattaagat cgatgaggtt
    93661 gtcaaaggaa gcccagctgc tcaggctggc ttgcaaaaag acgatgtgat cattggcgtc
    93721 aaccgcgatc gggtgaactc gattgctgaa atgcgtaaag tgctggcggc aaaaccggcc
    93781 ataatcgcct tgcaaatcat acgcggcaat gaaagcctct acctactaat gcgttaatgt
    93841 cgtaaaccgg gcatcaggct gacgtgtgat gtccggttaa ctcgtggtat gctgctgccg
    93901 ttcccttttt taacgatgcc tccatcatgt ttgtgaagct cttacgttcc gttgcgattg
    93961 gattaattgt cggcgctatt ctgctggttg ccatgccttc gctgcgcagc cttaacccgc
    94021 tttccactcc gcaatttgac agtaccgatg aaacgcctgc cagctataat ctggcggttc
    94081 gccgcgccgc gccagcggtg gttaacgttt ataaccgcgg tttgaacacc aactctcaca
    94141 accagcttga gatccgcacc ctgggatctg gtgtaatcat ggatcaacgc gggtatatca
    94201 tcaccaataa acacgtgatc aacgacgccg atcagatcat cgtcgcctta caggatggac
    94261 gtgtttttga agcgttactg gttggctctg actctctaac cgatctggcg gtgctgaaaa
    94321 ttaacgccac aggcggttta ccaacgattc cgatcaatcc acgccgcgta ccgcacattg
    94381 gcgatgtggt tctggcgatc ggtaacccgt acaacctcgg gcagaccatt acccagggga
    94441 ttatcagtgc cacgggtcga atcggtctga acccgaccgg gcggcaaaac ttcctgcaaa
    94501 ccgatgcttc cattaaccac ggtaactctg gcggcgcgct ggtgaactcg ctgggcgaac
    94561 tgatgggcat taacacgctg tcgtttgata agagtaacga tggcgaaacg ccggaaggta
    94621 tcggctttgc gattcctttc cagttagcga ccaaaattat ggataaactg atccgtgatg
    94681 gccgtgtgat ccgtggctac attggcattg gtggacgtga gatcgcccca ttacatgcgc
    94741 agagtggcgg catggatcaa ctgcaaggga tcgtggttaa cgaagtgtcg cctgatggcc
    94801 cagcggcaaa tgcaggcatc caggtcaacg atctgattat ctctgtgaat aacaaacccg
    94861 ctatttccgc cctggagaca atggatcagg tcgcggaaat tcgccctggt tcggtgatcc
    94921 cggttgtcgt gatgcgtgac gataaacagt taacattgca ggtcaccatt caggaatatc
    94981 cggcgaccaa ctaaggtcag cgtaaaaaaa caaaaaccgg agtctgtgct ccggtttttt
    95041 attatccgct aatcaattac ttattaacga actcttcgcc cagggcgata tctttcttca
    95101 gcgtatccag cataccttcc agcgcgttct gttcaaatgc gctcagagta ccgatagatt
    95161 tacgctcttc cacgccgttt ttacccagca gcagcggttg agagaagaaa cgagcgtact
    95221 gaccgtcgcc ttcaacgtag gcacattcga caacgccttg ttcgccctgc agtgcgcgaa
    95281 ccagagacag accaaaacgt gcagctgcct ggcccataga cagggttgca gacccgccac
    95341 cggctttcgc ttcaaccacc tcagtacccg cgttctggat acgtttggtc agatcagcca
    95401 cttcctgctc ggtaaaacta acgccaggaa cctgtgacag cagcggcaga atggtaacac
    95461 cagagtgacc accaataacc ggcacttcaa cttcgcctgg ctgtttgcct ttcagttccg
    95521 caacaaaggt gttggaacga atgatatcca gcgtggtaac gccgaacagt ttgtttttgt
    95581 cataaacacc ggcttttttc agcacttcag cagcaatcgc aactgtagtg ttaaccgggt
    95641 tagtgataat accaatgcac gctttcgggc aggttttcgc aacttgctgt accaggtttt
    95701 tcacgatacc ggcgttaacg ttaaacaggt cggaacgatc catacccggt ttacgcgcta
    95761 cgcctgcaga gataagaacg acatctgcac cttccagcgc cggagtcgca tcttcacctg
    95821 aaaaaccttt gattttcaca gcagtaggga tatggctcag atcgacagcc acaccgggag
    95881 tcactggagc gatatcatac agagagagtt ctgaacctga aggcagttgg gtttttaaca
    95941 gtagtgcaag cgcctggcca ataccgccag cagcgccgag gactgcgact ttcatcctaa
    96001 actccttatt atattgataa actaagatat gttgctccgc tgccgcgacc ttaatccaca
    96061 aaattgccgt ttacaatgac cacatctcaa gaatgtgtag tcacgcaagt ttagcgttta
    96121 tgcatttaat tgccgtaatc aggaacctaa cttacgtaat taacagtcac ttatgcatta
    96181 gcgcaacatt ccaacaggtg gtgacaatat accctaccgc tcagccaaaa caatatcaat
    96241 ttgataacaa ttaatttact tttaagcaga atttgcaggc cgtgacgcag gcatgtttct
    96301 caataacgaa atttgataaa atcccgctct ttcataacat tatttcagcc ttcttcaggg
    96361 ctgactgttt gcataaaaat tcatctgtat gcacaataat gttgtatcaa ccaccatatc
    96421 gggtgactta tgcgaagctc ggctaagcaa gaagaactag ttaaagcatt taaagcatta
    96481 cttaaagaag agaaatttag ctcccagggc gaaatcgtcg ccgcgttgca ggagcaaggc
    96541 tttgacaata ttaatcagtc taaagtctcg cggatgttga ccaagtttgg tgcggtacgt
    96601 acacgcaatg ccaaaatgga aatggtttac tgcctgccag ctgaactggg tgtaccaacc
    96661 acctccagtc cattgaagaa tctggtactg gatatcgact acaacgatgc agttgtcgtg
    96721 attcatacca gccctggtgc ggcgcagtta attgctcgcc tgctggactc actgggcaaa
    96781 gcagaaggta ttctgggcac catcgctggc gatgacacca tctttactac ccctgctaac
    96841 ggtttcaccg tcaaagagct gtacgaagcg attttagagc tgttcgacca ggagctttaa
    96901 tctctgcccc gtcgcttctg gcggcgggga aaatgttgct tttgcctatc aacccactgc
    96961 tttcccctgc gattcattta acgaatagtg cgttttactg cgacata
//
LOCUS       DAHHTG010000003        79443 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.003, whole genome shotgun sequence.
ACCESSION   DAHHTG010000003 DAHHTG010000000
VERSION     DAHHTG010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 79443)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 79443)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..79443
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.003"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            complement(32..313)
                     /locus_tag="NBI74_000219"
     CDS             complement(32..313)
                     /locus_tag="NBI74_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehE family protein"
                     /protein_id="HCD1930602.1"
                     /translation="MNKYWLSGIIFLAYGLASPAFSSETATLTINGRISPPTCSMAMV
                     NGQPQQHCGQLTYNVDTRHQVSSPVKGVTTEVIAADSDSKRRIVLNRYD"
     gene            complement(576..1685)
                     /gene="apbC"
                     /locus_tag="NBI74_000220"
     CDS             complement(576..1685)
                     /gene="apbC"
                     /locus_tag="NBI74_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HCD1930603.1"
                     /translation="MNEQSQAKSPEALRAMVAGTLANFQHPTLKHNLTTLKALHHVAW
                     MDDTLHVELVMPFVWHSAFEELKEQCSAELLRITGAKAIDWKLSHNIATLKRVKNQPG
                     INGVKNIIAVSSGKGGVGKSSTAVNLALALAAEGAKVGILDADIYGPSIPTMLGAENQ
                     RPTSPDGTHMAPIMSHGLATNSIGYLVTDDNAMVWRGPMASKALMQMLQETLWPDLDY
                     LVLDMPPGTGDIQLTLAQNIPVTGAVVVTTPQDIALIDAKKGIVMFEKVEVPVLGIVE
                     NMSVHICSNCGHHEPIFGTGGAEKLAEKYHTQLLGQMPLHISLREDLDKGTPTVISRP
                     ESEFTAIYRQLADRVAAQLYWQGEVIPGEISFRAV"
     gene            1817..3850
                     /gene="metG"
                     /locus_tag="NBI74_000221"
     CDS             1817..3850
                     /gene="metG"
                     /locus_tag="NBI74_000221"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HCD1930604.1"
                     /translation="MTQVAKKILVTCALPYANGSIHLGHMLEHIQADVWVRYQRMRGH
                     EVNFICADDAHGTPIMLKAQQLGITPEQMIGEMSQEHQTDFAGFNISYDNYHSTHSEE
                     NRQLSELIYSRLKENGFIKNRTISQLYDPEKGMFLPDRFVKGTCPKCKSPDQYGDNCE
                     VCGATYSPTELIEPKSVVSGATPVMRDSEHFFFDLPSFSEMLQAWTRSGALQEQVANK
                     MQEWFESGLQQWDISRDAPYFGFEIPNAPGKYFYVWLDAPIGYMGSFKNLCDKRGDSV
                     SFDEYWKKDSTAELYHFIGKDIVYFHSLFWPAMLEGSNFRKPTNLFVHGYVTVNGAKM
                     SKSRGTFIKASTWLNHFDADSLRYYYTAKLSSRIDDIDLNLEDFVQRVNADIVNKVVN
                     LASRNAGFINKRFDGVLASELADPQLYKTFTDAAEVIGEAWESREFGKAIREIMALAD
                     LANRYVDEQAPWVVAKQEGRDADLQAICSMGINLFRVLMTYLKPVLPKLTERAEAFLN
                     TELTWDGIQQPLLGHKVNPFKALYNRIDMKQVEALVEASKEEVKAAAAPVTGPLADDP
                     IQETITFDDFAKVDLRVALIENAEFVEGSDKLLRLTLDLGGEKRNVFSGIRSAYPDPQ
                     ALIGRHTIMVANLAPRKMRFGISEGMVMAAGPGGKDIFLLSPDAGAKPGHQVK"
     gene            3991..7791
                     /locus_tag="NBI74_000222"
     CDS             3991..7791
                     /locus_tag="NBI74_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001615297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WGR and DUF4132 domain-containing protein"
                     /protein_id="HCD1930605.1"
                     /translation="MRHFIYQDEKSHKFWAVEQQGNELHVSWGKVGTNGQNQVKSFAD
                     AAAAEKAKLKLIAEKTKKGYVENASANVHITPITKATPEVETSPESKNQRPWLADDAV
                     IRLTDDINRFAFPHRSRPREINYLHKDSQIWQRIANNTRAYDPANNYRSYPENWQQAF
                     AELQRRILDNQQTGSAQSDAALLWSFWNSYSADELVDDLIIRCGMESAVEIALLALQL
                     RYKPVKGAVTTIIPPNHEAESLPSWHQRLCHHLSLASEDEWQRCVDKVLAAITTLSPA
                     RQPFAALLLPERPDIANAMALRYADQNVPAMTWLRLVVTDEAALTAAEKYDFPPLYRD
                     FRAYLAMLLANNGVSGVSRILTEFVEEHSDNPTYLFERISETEDLVKWLWKTNHPDAI
                     QILILGVIGKKKHLEYLSKACQKHPAAAIAAYATLLAIHEDKEWHKALVKLITITPEL
                     VCDVIPWVSAKAAGILSECRPQSVAEECEYATADMLPELFVSPPWMTKEKKKNSPVFD
                     LPVLPVPSVSDVTPEIAKKLTRTYLVTRFQQIAQQQATKQILFTDLPPMEKASWERHL
                     VPLTPEQQILWCLGFDKWRESGEKIYEKIPAPQSAVDALLRFDFPALNAEFAQYHNNA
                     YKSWNLIALCYLPGQQAISFLNQIVKEDNYSGEGYILAIFGSAAIPAFMTCLQREPQR
                     LWFFTFFLGVNELALPMAQRLQKKMSYEDARNWLTDYPRHAAAGLLPVALGKQGKDRD
                     CARQALRLLVNLNQRETIEEIAQGYNQPDVLAALATLFDSEPLEEYPAKIAPLPGFYQ
                     FTLWRRPRLKSNNQPLPDDAMRHLGIMLSFPRDITPYAGLAIIKETFTRESLAEFGWD
                     LYTAWTEAGAPAKENWAFTSLGILGNDDTARKLTPLIRTWPGESQHKRAVYGLDVLAS
                     IGSDIALMLLNGIAKKIKFVALQEHACDKINMVAENRGLTMAELEDRLAPDLGLDSSG
                     SLTLDFGPRQFTVGFDETLKPVVRDANGKVLKDLPKPNQSDDKTLATDAVNLFKQLKK
                     DVRAIASQQIDRLEQAMCQRRRWTAEQFRLFLVEHPLVRHLTRRLLWGVYTEENTLIA
                     CFRVAEDSTYSDAQDELFTLPAGNIGIPHVLEISPESAAAFGQIYTDYEQLPPFRQLD
                     RGYYHLADNERDSHELIRWQGRLCQAGRIVGLERRGWQRLEESGSVYAMRKSTPYGDL
                     ELETEPFSLIYGETGYGDQLPVESVKITSPDNRYGKQSSLTFSALDDITASELINDIE
                     SLFD"
     gene            7804..11565
                     /locus_tag="NBI74_000223"
     CDS             7804..11565
                     /locus_tag="NBI74_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000860751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WGR and DUF4132 domain-containing protein"
                     /protein_id="HCD1930606.1"
                     /translation="MKTYIYQDEKSHKFWAVEQQENELHISWGKVGTNGQSQIKSFAD
                     AAAAEKAELKLIAEKTKKGYAEEVSVTTPTSAPVQVIACSEKAPLPQDKPPFAEDHLP
                     WLADDAQIILPTEVAPTTLSHRRWPGDSVPQENELTLLRSVAAHTHRRFKKVITFDYS
                     TCSLDWQQAITQAVGLIDSPISTALPPMVLAVLVALEQGFNRNDHEELMDQIVQEGGL
                     EYATEVVIALQAIRFDWNYDAHLITFTPDNKQPGYLSRFASVEMRLRKHLSLANDDVW
                     QRCADKLIAALGNIPAWHQPLVALLLPEKQDVSHEIARRLCGQKGLYALEWLKLTAAD
                     EQVLAELGKYYPGQPGQVFDDYYGGKIWCATILKEQGVGALARFAPYAAGDTCGEVLM
                     HINHPQALTLLIHASEQGKRCHDRMTKTFVRFPHAALAALAELLAQKDQKRWRMMLMT
                     MLISQPTLAERVIPWLSTPAVAVLKSCQQQLTQPSNHASADMLPAVLVSPPWLSKKKK
                     SVMPVLDLTPLPLESCCTLTETAEKEIHARHRWHAHQIDIGQKEDIQNYLTRLGFNRW
                     NNGQYMKASDAVVELWQRGDYSALISEFKTFWHSYQREWQLYMLAALPIEKTAQAWNV
                     LSKEPHVGVEFVMTHLQLAGLQGFIHSFSRYPQEALPVAQYFAAIELAPLIARAFNKL
                     KTLRQDARIWLLKYPEHAITGLLPAALGKTGEAQDNARAALRMLTENGHQPLLQEIAR
                     RYNQPEVTDAVNALLALDPLDNHPTKIPTLPTFYQPSLWTRPLLKANAQSLPDSALLH
                     LGEMLRFPQEEALYPGLLQVKDACTTDSLAEFAWDLFTAWQTAGAPSKESWAFTALGV
                     LGNDDTARKLTPLIRAWPGESQHKRATVGLDILAAIGSDIALMQLNGIAQKLKFKALQ
                     ERAKEKIANIAESRELTVAELEDRLAPDLGLDDNGSLLLDFGPRQFTVSFDETLKPFV
                     RDASGSRLKDLPKPNKSDDESQANNAVNRYKLLKKDARTVAAQQVARLESAMCLRRRW
                     SPENFQLFLVEHPLVRHLTRRLIWGVYSAENQLLACFRVAEDNSYSTADDDLFTLPEG
                     DISIGIPHVLEISPTDAAAFGQLFADYELLPPFRQLDRNSYALTEAERNASELTRWAG
                     RKCPSGRVMGLANKGWMRGEPQDGGWIGWMIKPLGRWSLIMEIDEGFAVGMSPAELSA
                     EQLLSKLWLWEGKAESYGWGSNSTQEAQFSVLDAITASELINDIEALFE"
     gene            11575..15207
                     /locus_tag="NBI74_000224"
     CDS             11575..15207
                     /locus_tag="NBI74_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4132 domain-containing protein"
                     /protein_id="HCD1930607.1"
                     /translation="MDKELPWLADNAQLELKYKKGKTPLSHRNWPGEPVPVITENIIQ
                     TLGDELLQKAEKKKNIVWRYENFSLEWQSAITQAINLIGEHKPSITARTMAALACIAQ
                     NDSQQLLDEIVQQEGLEYATEVVIARQFIVRCYESDPLVVTLQYQNEDYGYGYRSETY
                     NEFDLRLRKHLSLAEESCWQRCADKLIAALPGITKVRRPFIALNLPEKPEIANELVSL
                     ECSQTHFRSKEWLKVVADDPKAVKELARYWSQDIFSDREASYMSHENHFGYAACAALL
                     REQGLAAVPRLAMYAHKEDCGSLLVQINHPQVIRSLLLVADKNKPSLQRVAKYSKNFP
                     HATLAALAELLALKAPPARPGYPIIEDKKLPAQQKARDEYWHTLLQTLMASQPQLAEE
                     VMPCLCTQAQAVVNGYLSASPKLAFESTHSNDLPEILVSPPWRGKKKTALLRLDLVPL
                     ELAPKARWQPGERERLAATESARYFSTGSFTERMERKSGRVVLQELGFGDDVWLFRNY
                     ILPGKLDAARKSLVGQWHYSPRRVEEINNGWHSTEAKSAEQALRSGDVEALINTWEND
                     SYSHYRQEKSVWNLYLLAQLPREMALTFWLRINEKKHLFAGEDYFLSILGLDTLPGLL
                     LAFSHHPKETFPLILNFGATELALPVARVWRRFAAQRDLARQWILQWPEHTATALIPL
                     VFTKPSDNSEAALLALRLLYEQGHGELLQTVANRWQRTDVWPALEQLLKLGPIEIYPA
                     RIPKAPDFWHPGMWSRPRLITNNQPVTDDALEIIGEMLRFTQGGRFYGGLEQLKTFCQ
                     PQTLAAFAWDLFTAWQQAGAPAKDNWAFLALSLFGDETTARDLTTQILAWPQEGKSAR
                     AVSGLNILTLMNNDMALIQLHHISQRAKSRSLRDNAAEFLQVVAENRGLSQEELADRL
                     VPTLGLDDPQALSFDFGPRQFTVRFDENLNPVIFDQQNVRQKSVPRLRADDDQLKAPE
                     ALARLKGLKKDATQVSKNLLPRLEAALRITRRWSLADFHSLFVNHPFTRLVTQRLIWG
                     VYPANEPRRLLNAFRVAAEGEFCNAQDEPIDLPADALIGIAHPLEMAAEMRSEFAQLF
                     ADYEIMPPFRQLARRTVLLTPDESASNSLTRWEGKSATVGQLMGMRYKGWESGYEDAF
                     VYDLGAYRLVLKFSPGFNHYNVDSKALMSFRSLRVYRDNKSVTFAELDVFDLSEALSA
                     PDVIFH"
     gene            15268..15584
                     /locus_tag="NBI74_000225"
                     /pseudo
     CDS             15268..15584
                     /locus_tag="NBI74_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588459.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            16286..17374
                     /locus_tag="NBI74_000226"
     CDS             16286..17374
                     /locus_tag="NBI74_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416623.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HCD1930608.1"
                     /translation="MSPQNNHLQRPPAAVLYAVELAKLKQNDNAPCPPGWQLSLPAAR
                     AFILGDEAQNIRRKVVISPSAVERMLVTLATGRGLMLVGEPGTAKSLLSELLATAISG
                     DAGLTIQGGASTTEDQIKYGWNYALLINHGPSTEALVPAPLYLGMRDGKIVRFEEITR
                     TPLEVQDCLLGMLSDRVMTVPELTGEASQLYAREGFNIIATANTRDRGVNEMSAALKR
                     RFDFETVFPIMDFAQELELVASASARLLAHSGIPHKVPDAVLELLVRTFRDLRANGEK
                     KTSMDTLTAIMSTAEAVNVAHAVGVRAWFLANRAGEPADLVDCIAGTIVKDNEEDRAR
                     LRRYFEQRVATHKEAHWQAYYQARHRLP"
     gene            17385..19664
                     /locus_tag="NBI74_000227"
     CDS             17385..19664
                     /locus_tag="NBI74_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1930609.1"
                     /translation="MSEPLIVGIRHHSPACARLVKSLIESQRPRYVLIEGPADFNDRV
                     DELFLAHQLPVAIYSYCQYQDGAAPGRGAWTPFAEFSPEWQALQAARRIQAQTYFIDL
                     PCWAQSEEEDDSPDTQDESQTLLLRATRMDNSDTLWDHLFEDESQQTALPSTLAHYFA
                     QLRGDSPGDALNRQREAFMARWITWAMQQNNGDVLVVCGGWHAPALAKMWRKCPQEMN
                     KPELSSLADGVTGCYLTPYSEKRLDVLAGYLSGMPAPVWQNWCWQCGLQQAGEQLLKT
                     VLTRLRQHKLPASTADMAAAHLHAMALAQLRGHTLPLRTDWLDAIAGSLIKEALNAPL
                     PWSYRGVIHPDTDPILLTVIDTLAGDGFGKLSPSTPQPPLPKDVTCELERTGISLPAE
                     LTLNRFTPDGLAQSQVLHRLAILEIPGVVRQQGSTLTLAGNGEECWKLTRPLSQHAAL
                     IEAACFGATLQEAARHKLEADMLDAGGIGCITTCLSQAALAGLASFSQQLLEQLTLLI
                     AQENQFAEMGQALEVLYALWRLDEISGMQGAQILQTTLCAAIDRTLWLCESNGRPDEK
                     EFHAHLHSWQALCHILRDLHSGVNLPGVSLSAAVALLERRSQAIHAPALDRGATLGAL
                     MRLEHPNASAEAALTMLAQLSPAQSGEALHGLLALARHQLACQPVFIAGFSSHLNQLS
                     DADFINALPDLRAAMAWLPPRERGTLAHQVLEHYQLTQLPVSALQMLLHCPPQAIAHH
                     QQLEQQALASLQHWGVFHV"
     gene            19657..20792
                     /locus_tag="NBI74_000228"
                     /pseudo
     CDS             19657..20792
                     /locus_tag="NBI74_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416625.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
     gene            20789..22792
                     /locus_tag="NBI74_000229"
     CDS             20789..22792
                     /locus_tag="NBI74_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HCD1930610.1"
                     /translation="MNSLRPELLELTPQALTALSNAGFVKRSLKELENGNVPEISHEN
                     GALIATFSDGVRTQLANGQALKEAQCTCGASGMCRHRVMLVLSYQRLCATAQPTEKKE
                     EEWDPAIWLKELANLPDATRKRAQALVAKGITIELFCAPGEIPSARLPMSDVRFYSRS
                     SIRFARCDCIEGTLCEHVVLAVQAFVEAKTQQAEFTHLIWQMRSEHVTSSDDPFASEE
                     GKTCRQYVQQLSQALWLGGISQPPIHYEAAFSRAQQAAERCNWRWVSESLRQLRASVD
                     AFHARASHYHAGECLRQLAALNSRLNCVQEMARRDSIGEVPPMPWRTVVGAGIAGEAK
                     LDHLRLVSLGMRCWQDIEQYGLRIWFTDPDTGSILHLSRSWQRSEQENSPAATRRLFS
                     FQAGALAGGQIVSQAAKRSADGELLLATRNRLSSVVPLSPDAWQMLSAPLRQPGIVAL
                     REYLRQRPPACIRPLNQVDNLFILPVAECISLGWDSSRQTLDAQVISGEGEDNVLTLS
                     LPASACSPFAVERMAALLQQTDDPVSLVSGFVSFVEGQLTLEPRVMMTKTRAWALDAE
                     TAPVAPLPSASVLPVPSTAHQLLMRCQALLIQLLHNGWRYQEQSAIGEAELLANDLTA
                     VGFYRLAHVLGQFRNTESEARVEAMNNGVLLCEQLFPMLQQQG"
     gene            22917..23378
                     /locus_tag="NBI74_000230"
     CDS             22917..23378
                     /locus_tag="NBI74_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehR family lipoprotein"
                     /protein_id="HCD1930611.1"
                     /translation="MKAFNKLFSLVVASVLVFSLAGCGDKEESKKFSANLNGTEIAIT
                     YVYKGDKVLKQSSETKIQFASIGATTKEDAARTLEPLSAKYKNIAGVEEKLTYTDTYA
                     QENVTIDMEKVDFKALQGISGINVSAEDAKKGITMAQMELVMKAAGFKEVK"
     gene            complement(23419..23889)
                     /locus_tag="NBI74_000231"
     CDS             complement(23419..23889)
                     /locus_tag="NBI74_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HCD1930612.1"
                     /translation="MLSNDILRSVRYILKANNNDLVRILALGNVEATAEQIAVWLRKE
                     DEEGFQRCPDIVLSSFLNGLIYEKRGKDESAPALEPERRINNNIVLKKLRIAFSLKTD
                     DILAILTEQQFRVSMPEITAMMRAPDHKNFRECGDQFLRYFLRGLAARQHVKKS"
     gene            complement(23936..24655)
                     /gene="yehT"
                     /locus_tag="NBI74_000232"
     CDS             complement(23936..24655)
                     /gene="yehT"
                     /locus_tag="NBI74_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310963.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BtsR"
                     /protein_id="HCD1930613.1"
                     /translation="MIKVLIVDDEPLARENLRVFLQEQSDIEIVGECSNAVEGIGAVH
                     KLRPDVLFLDIQMPRISGLEMVGMLDPEHRPYIVFLTAFDEYAIKAFEEHAFDYLLKP
                     IDEARLEKTLARLRQERSKQDVSLLPENQQALKFIPCTGHSRIYLLQMKDVAFVSSRM
                     SGVYVTSHEGKEGFTELTLRTLESRTPLLRCHRQYLVNLAHLQEIRLEDNGQAELILR
                     NGLTVPVSRRYLKSLKEAIGL"
     gene            complement(24652..26337)
                     /gene="btsS"
                     /locus_tag="NBI74_000233"
     CDS             complement(24652..26337)
                     /gene="btsS"
                     /locus_tag="NBI74_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulatory system sensor histidine
                     kinase BtsS"
                     /protein_id="HCD1930614.1"
                     /translation="MYDFNLVLLLLQQMCVFLVIAWLMSKTPLFIPLMQVTVRLPHKF
                     LCYIVFSIFCIMGTWFGLHIDDSIANTRAIGAVMGGLLGGPVVGGLVGLTGGLHRYSM
                     GGMTALSCMISTIVEGLLGGLVHSILIRRGRTDKVFNPITAGAVTFVAEMVQMLIILA
                     IARPYEDAVRLVSNIAAPMMVTNTVGAALFMRILLDKRAMFEKYTSAFSATALKVAAS
                     TEGILRQGFNEVNSMKVAQVLYQELDIGAVAITDREKLLAFTGIGDDHHLPGKPISST
                     YTLKAIETGEVVYADGNEVPYRCSLHPQCKLGSTLVIPLRGENQRVMGTIKLYEAKNR
                     LFSSINRTLGEGIAQLLSAQILAGQYERQKAMLTQSEIKLLHAQVNPHFLFNALNTIK
                     AVIRRDSEQASQLVQYLSTFFRKNLKRPSEFVTLADEIEHVNAYLQIEKARFQSRLQV
                     NIAIPQELSQQQLPAFTLQPIVENAIKHGTSQLLDTGRVAISARREGQHLMLEIEDNA
                     GLYQPVTNASGLGMNLVDKRLRERFGDDYGISVACEPDSYTRITLRLPWRDEA"
     gene            26559..27290
                     /gene="mlrA"
                     /locus_tag="NBI74_000234"
     CDS             26559..27290
                     /gene="mlrA"
                     /locus_tag="NBI74_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MlrA"
                     /protein_id="HCD1930615.1"
                     /translation="MALYTIGEVALLCDINPVTLRAWQRRYGLLKPQRTDGGHRLFND
                     ADIDRIREIKRWIDNGVQVSKVKMLLSNENVDVQNGWRDQQETLLTYLQSGNLHSLRT
                     WIKERGQDYPAQTLTTHLFIPLRRRLQCQQPTLQTLLAILDGVLINYISICLASARKK
                     QGKDALVVGWNIQDTTRLWLEGWIASQQGWRIDVLAHSLNQLRPELFEGRTLLVWCGD
                     NRTSAQQQQLTSWQEQGHDIFPLGI"
     gene            27350..27457
                     /locus_tag="NBI74_000235"
     CDS             27350..27457
                     /locus_tag="NBI74_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YohO"
                     /protein_id="HCD1930616.1"
                     /translation="MRIAKIGVIALFLFMALGGIGGVMLAGYTFILRAG"
     gene            complement(27438..28169)
                     /gene="yehW"
                     /locus_tag="NBI74_000236"
     CDS             complement(27438..28169)
                     /gene="yehW"
                     /locus_tag="NBI74_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehW"
                     /protein_id="HCD1930617.1"
                     /translation="MKMLRDPLFWLIALFVALIFWLPYSQPLFAALFPQLPRPVYQQE
                     SFAALALAHFWLVGISSLFAVIIGTGAGIAVTRPWGAEFRPLVETIAAVGQTFPPVAV
                     LAIAVPVIGFGLKPAIIALILYGVLPVLQATLAGLGAIDASVTEVAKGMGMSRGQRLR
                     KVELPLAAPVILAGVRTSVIINIGTATIASTVGASTLGTPIIIGLSGFNTAYVIQGAL
                     LVALAAIIADRLFERLVQAFSQHAK"
     gene            complement(28174..29100)
                     /gene="yehX"
                     /locus_tag="NBI74_000237"
     CDS             complement(28174..29100)
                     /gene="yehX"
                     /locus_tag="NBI74_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter ATP binding
                     protein YehX"
                     /protein_id="HCD1930618.1"
                     /translation="MIEFSHVSKLFGAQKAVNDLNLNFQEGSFSVLIGTSGSGKSTTL
                     KMINRLVEHDSGVIRFAGEEIRSLPVLELRRRMGYAIQSIGLFPHWSVAQNIATVPQL
                     QKWSRARIDDRIDELMALLGLEPNLRERYPHQLSGGQQQRVGVARALAADPQVLLMDE
                     PFGALDPVTRGALQQEMTRIHRLQGRTIVLVTHDIDEALRLAEHLVLMDHGEVVQQGN
                     PLAMLTHPANDFVRQFFGRSELGVRLLSLRSVADYVRREERAEGEALEERMTLRDALS
                     LFVARGCEVLPVVNTQGQPCGTLHFQDLLEEA"
     gene            complement(29093..30250)
                     /gene="yehY"
                     /locus_tag="NBI74_000238"
     CDS             complement(29093..30250)
                     /gene="yehY"
                     /locus_tag="NBI74_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehY"
                     /protein_id="HCD1930619.1"
                     /translation="MTYLRINPVLALLLLLTAIAAALPFISYAPNRLVSGEGRHLWQL
                     WPQTLWMLVGVGCAWLTACFIPAKKGSIFALILAQFVFVLLVWGAGKAATQLAQNGSA
                     LARTSLGSGFWLAAALALLACSDAIRRISTHPLWRWLLHMQIAIIPLWLLYSGTLNDL
                     SLMKEYANRQDVFDDALAQHLTLLFGAVLPALVIGVPLGIWCYFSTARQGAIFSLLNV
                     IQTVPSVALFGLLIAPLAALVTAFPWLGTLGIAGTGMTPALIALVLYALLPLVRGVVV
                     GLNQIPRDVLESARAMGMSGVQRFLHVQLPLALPVFLRSLRVVMVQTVGMAVIAALIG
                     AGGFGALVFQGLLSSAIDLVLLGVIPVIVLAVLTDALFDLLIALLKVKRND"
     gene            complement(30257..31174)
                     /gene="osmF"
                     /locus_tag="NBI74_000239"
     CDS             complement(30257..31174)
                     /gene="osmF"
                     /locus_tag="NBI74_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein OsmF"
                     /protein_id="HCD1930620.1"
                     /translation="MPLSKVWAGSLVLLAAVSLPLQAASPVKVGSKIDTEGALLGNII
                     LQVLESHGVPTVNKVQLGTTPVVRGAITSGELDIYPEYTGNGAFFFKDENDAAWKNVQ
                     QGYEKVKKLDAEQNKLIWLTPAPANNTWTIAVRQDVAEKNKLTSLADLSRYLQEGGTF
                     KLAASAEFIERADALPAFEKAYGFKLGQDQLLSLAGGDTAVTIKAAAQQTSGVNAAMA
                     YGTDGPVAALGLQTLSDPQGVQPIYAPAPVVRESVLKEYPQMAQWLQPVFASLDEKTL
                     QQLNASIAVEGLDAKKVAADYLKQKGWTK"
     gene            complement(31364..33661)
                     /gene="bglX"
                     /locus_tag="NBI74_000240"
     CDS             complement(31364..33661)
                     /gene="bglX"
                     /locus_tag="NBI74_000240"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucosidase BglX"
                     /protein_id="HCD1930621.1"
                     /translation="MKWLCSVGIAVSLALQPALADDLFGNHPLTPEARDAFVTELLKK
                     MTVDEKIGQLRLISVGPDNPKEAIREMIKDGQVGAIFNTVTRQDIRAMQDQVMELSRL
                     KIPLFFAYDVLHGQRTVFPISLGLASSFNLDAVKTVGRVSAYEAADDGLNMTWAPMVD
                     VSRDPRWGRASEGFGEDTYLTSIMGKTMVEAMQGKSPADRYSVMTSVKHFAAYGAVEG
                     GKEYNTVDMSPQRLFNDYMPPYKAGLDAGSGAVMVALNSLNGTPATSDSWLLKDVLRD
                     QWGFKGITVSDHGAIKELIKHGTAADPEDAVRVALKSGINMSMSDEYYSKYLPGLIKS
                     GKVTMEELDDAARHVLNVKYDMGLFNDPYSHLGPKESDPVDTNAESRLHRKEAREVAR
                     ESLVLLKNRLETLPLKKSATIAVVGPLADSKRDVMGSWSAAGVADQSVTVLTGIKNAV
                     GENGKVLYAKGANVTSDKGIIDFLNQYEEAVKVDPRSPQEMIDEAVQTAKQSDVVVAV
                     VGEAQGMAHEASSRTDITIPQSQRDLIAALKATGKPLVLVLMNGRPLALVKEDQQADA
                     ILETWFAGTEGGNAIADVLFGDYNPSGKLPMSFPRSVGQIPVYYSHLNTGRPYNADKP
                     NKYTSRYFDEANGALYPFGYGLSYTTFTVSDVKLSAPTMKRDGKVTASVQVTNTGKRE
                     GATVVQMYLQDVTASMSRPVKQLKGFEKITLKPGETQTVSFPIDIEALKFWNQQMKYD
                     AEPGKFNVFIGTDSARVKKAEFELL"
     gene            33857..35572
                     /gene="dld"
                     /locus_tag="NBI74_000241"
     CDS             33857..35572
                     /gene="dld"
                     /locus_tag="NBI74_000241"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HCD1930622.1"
                     /translation="MSSMTTTDNKAFLNELSRLVGHSHLLTDPAKTARYRKGFRSGQG
                     DALAVVFPGSLLELWRVLKACVTADKIILMQAANTGLTEGSTPNGNDYDRDIVIISTL
                     RLDKLHVLGKGEQVLAYPGTTLYSLEKALKPLGREPHSVIGSSCIGASVIGGICNNSG
                     GSLVQRGPAYTEMSLFARINEDGKLTLVNHLGIDLGETPEQILSKLDDDRIKDDDVRH
                     DGRHAHDYDYVHRVRDIEADTPARYNADPDRLFESSGCAGKLAVFAVRLDTFEAEKNQ
                     QVFYIGTNQPEVLTEIRRHILANFENLPVAGEYMHRDIYDIAEKYGKDTFLMIDKLGT
                     DKMPFFFNLKGRTDAMLEKVKFFRPHFTDRAMQKFGHLFPSHLPPRMKNWRDKYEHHL
                     LLKMAGDGVGEAKSWLVDYFKQAEGDFFVCTPEEGSKAFLHRFAAAGAAIRYQAVHSD
                     EVEDILALDIALRRNDTEWYEHLPPEIDSQLVHKLYYGHFMCYVFHQDYIVKKGVDVH
                     ALKEQMLELLQQRGAQYPAEHNVGHLYKAPETLQKFYRENDPTNSMNPGIGKTSKRKN
                     WQEVE"
     gene            complement(35611..36543)
                     /gene="pbpG"
                     /locus_tag="NBI74_000242"
     CDS             complement(35611..36543)
                     /gene="pbpG"
                     /locus_tag="NBI74_000242"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416638.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine endopeptidase"
                     /protein_id="HCD1930623.1"
                     /translation="MPKFRVSLFSLALMLAVPFAPQAVAKTAAATTASQPEIASGSAM
                     IVDLNTNKVIYSNHPDLVRPIASISKLMTAMVVLDARLPLDEKLKVDISQTPEMKGVY
                     SRVRLNSEISRKDMLLLALMSSENRAAASLAHHYPGGYKAFIKAMNAKAKSLGMNNTR
                     FVEPTGLSVHNVSTARDLTKLLIASKQYPLIGQLSTTREDMATFSNPTYTLPFRNTNH
                     LVYRDNWNIQLTKTGFTNAAGHCLVMRTVINNKPVALVVMDAFGKYTHFADASRLRTW
                     IETGKVMPVPAAALSYKKQKAAQMAAAGQTAQND"
     gene            complement(36717..37304)
                     /locus_tag="NBI74_000243"
     CDS             complement(36717..37304)
                     /locus_tag="NBI74_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014170891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YIP1 family protein"
                     /protein_id="HCD1930624.1"
                     /translation="MSHVWGLFSHPDREMQVINRENETISHHYTHHVLLMAAIPVICA
                     FIGTTQIGWNFGDGTILKLSWFTGLALAVLFYGVMLAGVAVMGRVIWWMARNYPQRPS
                     LAHCMVFAGYVATPLFLSGLVALYPLVWLCALVGTVALFYTGYLLYLGIPSFLNINKE
                     EGLSFSSSTLAIGVLVLEVLLALTVILWGYGYRLF"
     gene            37474..38052
                     /locus_tag="NBI74_000244"
     CDS             37474..38052
                     /locus_tag="NBI74_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCD1930625.1"
                     /translation="MDLNTLISQYGYAALVIGSLAEGETVTLLGGVAAHQGLLKFPLV
                     VLSVALGGMIGDQVLYLCGRRFGGKLLRRFSKHQDKIERAQKLIQRHPYLFVIGTRFM
                     YGFRVIGPTLIGASQLPPKIFLPLNILGAFAWALIFTTIGYAGGQVIAPWLHNLDQHL
                     KHWVWLILVVVLVVGVRWWLKRRGKKKPDNQA"
     gene            complement(38182..38943)
                     /locus_tag="NBI74_000245"
     CDS             complement(38182..38943)
                     /locus_tag="NBI74_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCD1930626.1"
                     /translation="MAQVAIITASDSGIGKECALLLAQQGFDIGITWHSDEEGAKDTA
                     REVVSHGVRAEIVQLDLGNLPEGALALEKLIQRLGRIDVLVNNAGAMTKAPFLDMAFD
                     EWRKIFTVDVDGAFLCSQIAARQMVKQGQGGRIINITSVHEHTPLPDASAYTAAKHAL
                     GGLTKAMALELVRHKILVNAVAPGAIATPMNGMDDGDVKPGAEPSIPLRRFGATHEIA
                     SLVVWLCSEGANYTTGQSLIVDGGFMLANPQFNPE"
     gene            complement(38996..40432)
                     /gene="mdtQ"
                     /locus_tag="NBI74_000246"
     CDS             complement(38996..40432)
                     /gene="mdtQ"
                     /locus_tag="NBI74_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001583680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug resistance outer membrane protein
                     MdtQ"
                     /protein_id="HCD1930627.1"
                     /translation="MNRDSFYPAIACFPLLLMLAGCAPMHETRQALSQQTPAAQVDTA
                     LPTALKNGWPDSQWWLEYHDNQLTSLINNALQNAPDMQVAEQRIQLAEAQAKAVATQD
                     GPQIDFSADMERQKMSAEGLMGPFALNDPAAGTTGPWYTNGTFGLTAGWHLDIWGKNR
                     AEVTARLGTVKARAAEREQTRQLLAGSVARLYWEWQTQAALNTVLQQIEKEQNTIIAT
                     DRQLYQNGITSSVEGVETDINASKTRQQLNDVAGKMKIIEARLSALTNHQTKALKLNP
                     VALPKVASQLPDELGYSLLARRADLQAAHWYVESSLSTIDAAKAAFYPDINLMAFLQQ
                     DALHLSDLFRHSAQQMGVTAGLTLPIFDSGRLNANLDIAKAESNLSIASYNKAVVEAV
                     NDVARAASQVQTLAEKNQHQAQIERDALRVVGLAQARFNAGIIAGSRVSEARIPALRE
                     RANGLLLQGQWLDASIQLTGALGGGYKR"
     gene            40656..40739
                     /gene="yohP"
                     /locus_tag="NBI74_000247"
     CDS             40656..40739
                     /gene="yohP"
                     /locus_tag="NBI74_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YohP"
                     /protein_id="HCD1930628.1"
                     /translation="MKIILWAVLIIFLIGLLVVTGVFKMIF"
     gene            complement(41127..42077)
                     /gene="dusC"
                     /locus_tag="NBI74_000248"
     CDS             complement(41127..42077)
                     /gene="dusC"
                     /locus_tag="NBI74_000248"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(16) synthase DusC"
                     /protein_id="HCD1930629.1"
                     /translation="MRVLLAPMEGVLDSLVRELLTEVNDYDLCITEFVRVVDQLLPVK
                     VFHRICPELQNASRTPSGTLVRVQLLGQFPQWLAENAARAVELGSWGVDLNCGCPSKT
                     VNGSGGGATLLKDPELIYQGAKAMREAVPAHLPVSVKVRLDWDSGEKKFEIADAVQQA
                     GATELVVHGRTKEQGYRAEHIDWQAIGEIRQRLNIPVIANGEIWDWQSAQQCMAISGC
                     DAVMIGRGALNIPNLSRVVKYNEPRMPWPEVVALLQKYTRLEKQGDTGLYHVARIKQW
                     LSYLRKEYDEATELFQHVRVLNNSPDIARAIQAIDIDKLR"
     gene            42316..42714
                     /locus_tag="NBI74_000249"
     CDS             42316..42714
                     /locus_tag="NBI74_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HCD1930630.1"
                     /translation="MSKTLNIIWQYLRAFVLIYACLYAGIFIASLLPVTIPGSIIGML
                     ILFVLLALQILPAKWVNPGCYVLIRYMALLFVPIGVGVMQYFDLLRAQFGPVVVSCAV
                     STLVVFLVVSWSSQLVHGERKVVGQKGSEE"
     gene            42711..43406
                     /locus_tag="NBI74_000250"
     CDS             42711..43406
                     /locus_tag="NBI74_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidB/LrgB family autolysis modulator"
                     /protein_id="HCD1930631.1"
                     /translation="MMANIWWSLPLTLIVFFAARKLAARYKFPLLNPLLVAMVVIIPF
                     LMLTGISYDSYFKGSEVLNDLLQPAVVALAYPLYEQLHQIRARWKSIITICFIGSVVA
                     MVTGTSVALLMGASPEIAASILPKSVTTPIAMAVGGSIGGIPAISAVCVIFVGILGAV
                     FGHTLLNAMRIRTKAARGLAMGTASHALGTARCAELDYQEGAFSSLALVLCGIITSLI
                     APFLFPIILAVMG"
     gene            43536..44420
                     /gene="cdd"
                     /locus_tag="NBI74_000251"
     CDS             43536..44420
                     /gene="cdd"
                     /locus_tag="NBI74_000251"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HCD1930632.1"
                     /translation="MHPRFQTAFAQLADNLQSALEPILADKYFPALLTGEQVSSLKSA
                     TGLDEDALAFALLPLAAACARTPLSNFNVGAIARGVSGTWYFGANMEFIGATMQQTVH
                     AEQSAISHAWLSGEKALAAITVNYTPCGHCRQFMNELNSGLDLRIHLPGREAHALRDY
                     LPDAFGPKDLEIKTLLMDEQDHGYALTGDALSQAAIAAANRSHMPYSKSPSGVALECK
                     DGRIFSGSYAENAAFNPTLPPLQGALILLNLKGYDYPDIQRAVLAEKADAPLIQWDAT
                     SATLKALGCHNIDRVLLA"
     gene            44570..45289
                     /gene="sanA"
                     /locus_tag="NBI74_000252"
     CDS             44570..45289
                     /gene="sanA"
                     /locus_tag="NBI74_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane permeability protein SanA"
                     /protein_id="HCD1930633.1"
                     /translation="MLKRVFLSLLVLIGLLLLTVLGLDRWMSWKTAPYIYDELQDLPY
                     RQVGVVLGTAKYYRTGVINQYYRYRIQGAINAYNSGKVNYLLLSGDNALQSYNEPMTM
                     RKDLIAAGVDPSDIVLDYAGFRTLDSIVRTRKVFDTNDFIIITQRFHCERALFIALHM
                     GIQAQCYAVPSPKDMLSVRIREFAARFGALADLYIFKREPRFLGPLVPIPAMHQVPED
                     AQGYPAVTPEQLLELQKKQGK"
     gene            45292..45531
                     /locus_tag="NBI74_000253"
     CDS             45292..45531
                     /locus_tag="NBI74_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2542 family protein"
                     /protein_id="HCD1930634.1"
                     /translation="MDVQQFFVVAVFFLIPIFCFREAWKGWRAGAIDKRVKNAPEPVY
                     VWRAKNPGLFFAYMVAYIGFGILSIGMIVYLIFYR"
     gene            45725..46963
                     /gene="preT"
                     /locus_tag="NBI74_000254"
     CDS             45725..46963
                     /gene="preT"
                     /locus_tag="NBI74_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreT"
                     /protein_id="HCD1930635.1"
                     /translation="MPQQNYLDELTPAFTPLLAIKEASRCLLCHDAPCSQACPAQTDP
                     GKFIRSIYFRNFKGAAETIRENNALGAVCARVCPTEKLCQSGCTRAGVDAPIDIGRLQ
                     RFVTDFEQQTGMEIYQPGTKTLGKVAIIGAGPAGLQASVTLTNQGYDVTIYEKEAQPG
                     GWLRNGIPQFRLPQSVLDAEIARIEKMGVTIKCNNEIGKTLTLEQLKAENRAVLVTVG
                     LSSGSGLSLFEHSDVEIAVDFLQRARQAQGDISIQQSALIIGGGDVAMDVASTLKVLG
                     CQAVTCVAREELDEFPASEKEFTSARELGVSIIDGFTPVAVEGNKVTFKHVRLPGELT
                     IAADKIILAVGQHAELAAFSALEPQRNTIETQHYQTRDPQVFAAGDIVEGDKTVVYAV
                     KTGKEAAGAIHHYLEGACSC"
     gene            46957..48192
                     /gene="preA"
                     /locus_tag="NBI74_000255"
     CDS             46957..48192
                     /gene="preA"
                     /locus_tag="NBI74_000255"
                     /EC_number="1.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreA"
                     /protein_id="HCD1930636.1"
                     /translation="MLTKDLSITFCGVKFPNPFCLSSSPVGNCYEMCAKAYDTGWGGV
                     VFKTIGFFIANEVSPRFDHLVKEDTGFIGFKNMEQIAEHPLEENLAALRRLKEDYPDK
                     VLIASIMGENEQQWEELARLVQEAGADMIECNFSCPQMTSHAMGSDVGQSPELVEKYC
                     RAVKRGSTLPMLAKMTPNIGDMCEVALAAKRGGADGIAAINTVKSITNIDLNQKIGMP
                     IVNGKSSISGYSGKAVKPIALRFIQQMRTHPELRDFPISGIGGIETWEDAAEFLLLGS
                     ATLQVTTGIMQYGYRIVEDMASGLSHYLADQGFDSLQEMVGLANHNIVPAEDLDRSYI
                     VYPHINLDKCVGCGRCYISCYDGGHQAMEWSEKTRTPHCNTEKCVGCLLCGHVCPVGC
                     IDLGEVKFKKGEKEHPVTL"
     gene            complement(48435..49445)
                     /gene="mglC"
                     /locus_tag="NBI74_000256"
     CDS             complement(48435..49445)
                     /gene="mglC"
                     /locus_tag="NBI74_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     permease MglC"
                     /protein_id="HCD1930637.1"
                     /translation="MSALNKKSFLTYLKEGGIYVVLLVLLAIIIFQDPTFLSLLNLSN
                     ILTQSSVRIIIALGVAGLIVTQGTDLSAGRQVGLAAVVAATLLQSMDNANKVFPEMAT
                     MPIALVILIVCAIGAVIGLINGLIIAYLNVTPFITTLGTMIIVYGINSLYYDFVGASP
                     ISGFDSGFSTFAQGFIALGSFRLSYITFYALIAVVFVWVLWNKTRFGKNIFAIGGNPE
                     AAKVSGVNVGLNLLMIYALSGVFYAFGGMLEAGRIGSATNNLGFMYELDAIAACVVGG
                     VSFSGGVGTVIGVVTGVIIFTVINYGLTYIGVNPYWQYIIKGAIIIFAVALDSLKYAR
                     KK"
     gene            complement(49461..50981)
                     /gene="mglA"
                     /locus_tag="NBI74_000257"
     CDS             complement(49461..50981)
                     /gene="mglA"
                     /locus_tag="NBI74_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020883197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     ATP-binding protein MglA"
                     /protein_id="HCD1930638.1"
                     /translation="MVSSTTPSSGEYLLEMSGINKSFPGVKALDNVNLKVRPHSIHAL
                     MGENGAGKSTLLKCLFGIYQKDSGTILFQGKEIDFHSAKEALENGISMVHQELNLVLQ
                     RSVMDNMWLGRYPTKGMFVDQDKMYRETKAIFDELDIDIDPRARVGTLSVSQMQMIEI
                     AKAFSYNAKIVIMDEPTSSLTEKEVNHLFTIIRKLKERGCGIVYISHKMEEIFQLCDE
                     VTVLRDGQWIATEPLAGLTMDKIIAMMVGRSLNQRFPDKENKPGEVILEVRNLTSLRQ
                     PSIRDVSFDLHKGEILGIAGLVGAKRTDIVETLFGIREKSAGTITLHGKKINNHNANE
                     AINHGFALVTEERRSTGIYAYLDIGFNSLISNIRNYKNKVGLLDNSRMKSDTQWVIDS
                     MRVKTPGHRTQIGSLSGGNQQKVIIGRWLLTQPEILMLDEPTRGIDVGAKFEIYQLIA
                     ELAKKGKGIIIISSEMPELLGITDRILVMSNGLVSGIVDTKTTTQSEILRLASLHL"
     gene            complement(51042..52040)
                     /gene="mglB"
                     /locus_tag="NBI74_000258"
     CDS             complement(51042..52040)
                     /gene="mglB"
                     /locus_tag="NBI74_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/glucose ABC transporter
                     substrate-binding protein MglB"
                     /protein_id="HCD1930639.1"
                     /translation="MNKKVLTLSAVMASMLFGAAAHAADTRIGVTIYKYDDNFMSVVR
                     KAIEQDAKAAPDVQLLMNDSQNDQSKQNDQIDVLLAKGVKALAINLVDPAAAGTVIEK
                     ARGQNVPVVFFNKEPSRKALDSYDKAYYVGTDSKESGIIQGDLIAKHWAANQGWDLNK
                     DGQIQFVLLKGEPGHPDAEARTTYVIKELNDKGIKTEQLQLDTAMWDTAQAKDKMDAW
                     LSGPNANKIEVVIANNDAMAMGAVEALKAHNKSSIPVFGVDALPEALALVKSGALAGT
                     VLNDANNQAKATFDLAKNLADGKGAADGTNWKIDNKVVRVPYVGVDKDNLAEFSKK"
     gene            complement(52320..53360)
                     /gene="galS"
                     /locus_tag="NBI74_000259"
     CDS             complement(52320..53360)
                     /gene="galS"
                     /locus_tag="NBI74_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708050.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalS"
                     /protein_id="HCD1930640.1"
                     /translation="MITIRDVARQAGVSVATVSRVLNNSTLVSADTREAVMKAVSELD
                     YRPNANAQALATQVSDTIGVVVMDVSDAFFGALVKAVDLVAQQHQKYVLIGNSYHEAE
                     KERHAIEVLIRQRCNALIVHSKALSDDELAQFMDNIPGMVLINRVVPGYAHRCVCLDN
                     LSGARMATRMLLNNGHQRIGYLSSSHGIEDDAMRKAGWMSALKEQDIIPPESWIGTGT
                     PDMPGGEAAMVELLGRNLQLTAVFAYNDNMAAGALTALKDNGIAIPLHLSIIGFDDIP
                     IARYTDPQLTTVRYPIASMAKLATELALQGAADNIDPRASHCFMPTLVRRHSVATRQN
                     AAAITNSTNQAM"
     gene            complement(53502..54659)
                     /locus_tag="NBI74_000260"
     CDS             complement(53502..54659)
                     /locus_tag="NBI74_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF418 family protein"
                     /protein_id="HCD1930641.1"
                     /translation="MERNVTLDFVRGVAILGILLLNISAFGLPKAAYLNPAWYGAITP
                     QDAWTWAFLDLIGQVKFLTLFALLFGAGLQMLLPRGRRWIQSRLTLLVLLGFIHGLLF
                     WDGDILLAYGLVGLICWRLVRDAPSVKSLFNTGVMLYLVGLGVLLLLGLISDSQTSRA
                     WTPDASAILYEKYWKLHGGVEAINNRADGVGNSLLALGAQYGWQLAGMMLIGAALMRS
                     GWLKGQFSLRHYRRTGFVLVAIGVIINLPAIALQWQLDWAYRWCAFLLQMPRELSAPF
                     QAIGYASLFYGFWPQLSRFKLVLAIACVGRMALTNYLLQTLICTTLFYHLSLFMQFDR
                     LELLAFVIPVWLANIFFSVIWLRYFRQGPVEWLWRQLTLRAAGPTISKTSR"
     gene            complement(54676..55344)
                     /gene="folE"
                     /locus_tag="NBI74_000261"
     CDS             complement(54676..55344)
                     /gene="folE"
                     /locus_tag="NBI74_000261"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HCD1930642.1"
                     /translation="MPSLSKEAALVHEALVARGLETPLRPPVHEMDNETRKSLIAGHM
                     TEIMQLLNLDLADDSLMETPHRIAKMYVDEIFSGLDYANFPKITLIENKMKVDEMVTV
                     RDITLTSTCEHHFVTIDGKATVAYIPKDSVIGLSKINRIVQFFAQRPQVQERLTQQIL
                     IALQTLLGTNNVAVSIDAVHYCVKARGIRDATSATTTTSLGGLFKSSQNTRHEFLRAV
                     RHHN"
     gene            55602..56438
                     /gene="yeiG"
                     /locus_tag="NBI74_000262"
     CDS             55602..56438
                     /gene="yeiG"
                     /locus_tag="NBI74_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HCD1930643.1"
                     /translation="MEMLEEHRCFEGWQQRWRHDSSTLNCPMTFSIFLPPPRDHTPPP
                     VLYWLSGLTCNDENFTTKAGAQRVAAELGIVLVMPDTSPRGEQVANDDGYDLGQGAGF
                     YLNATQPPWATHYQMYDYLRDELPALIQSQFNVSDRCAISGHSMGGHGALIMALKNPG
                     KYTSVSAFAPIVNPCSVPWGIKAFSTYLGEDKNAWLEWDSCALMYASNAQDAIPTLID
                     QGDNDQFLADQLQPAVLAEAARQKAWPMTLRIQPGYDHSYYFIASFIEDHLRFHAQYL
                     LK"
     gene            complement(56470..58461)
                     /gene="cirA"
                     /locus_tag="NBI74_000263"
     CDS             complement(56470..58461)
                     /gene="cirA"
                     /locus_tag="NBI74_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore receptor CirA"
                     /protein_id="HCD1930644.1"
                     /translation="MFRLNPFVRVGLCLSAISCAWPVLAVDDDGETMVVTASSVEQNL
                     KDAPASISVITQEDLQRKPVQNLKDVLKEVPGVQLTNEGDNRKGVSIRGLDSSYTLIL
                     VDGKRVNSRNAVFRHNDFDLNWIPVDSIERIEVVRGPMSSLYGSDALGGVVNIITKKI
                     GQKWSGTVTVDTTVQEHRDRGDTYNGQFFTSGPLIDGVLGMKAYGSLAKREKDDPQNS
                     TTTDTGETPRIEGFSSRDGNVEFAWTPNQNHDFTAGYGFDRQDRDSDSLDKNRLERQN
                     YSVSHNGRWDYGTSELKYYGEKVENKNPGNSSPITSESNTVDGKYTLPLTAINQFLTV
                     GGEWRHDKLSDAVNLTGGTSSKTSASQYALFVEDEWRIFEPLALTTGVRMDDHETYGE
                     HWSPRAYLVYNATDTVTVKGGWATAFKAPSLLQLSPDWTSNSCRGACKIVGSPDLKPE
                     TSESWELGLYYMGEEGWLEGVESSVTVFRNDVKDRISISRTSDVNAAPGYQNFVGFET
                     GANGRRIPVFSYYNVNKARIQGVETELKIPFNDEWKLSINYTYNDGRDVSNGENKPLS
                     DLPFHTANGTLDWKPLALEDWSFYVSGHYTGQKRADSATAKTPGGYTIWNTGAAWQVT
                     KDVKLRAGVLNLGDKDLSRDDYSYNEDGRRYFMAVDYRF"
     gene            complement(58753..60222)
                     /gene="lysP"
                     /locus_tag="NBI74_000264"
     CDS             complement(58753..60222)
                     /gene="lysP"
                     /locus_tag="NBI74_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-specific permease"
                     /protein_id="HCD1930645.1"
                     /translation="MGSETKTTEAPGLRRELKARHLTMIAIGGSIGTGLFVASGATIS
                     QAGPGGALLSYMLIGLMVYFLMTSLGELAAYMPVSGSFATYGQNYVEEGFGFALGWNY
                     WYNWAVTIAVDLVAAQLVMSWWFPDTPGWIWSALFLGVIFLLNYISVRGFGEAEYWFS
                     LIKVTTVIVFIIVGVLMIIGIFKGAQPAGWSNWTIGEAPFAGGFAAMIGVAMIVGFSF
                     QGTELIGIAAGESEDPAKNIPRAVRQVFWRILLFYVFAILIISLIIPYTDPSLLRNDV
                     KDISVSPFTLVFQHAGLLSAAAVMNAVILTAVLSAGNSGMYASTRMLYTLACDGKAPR
                     IFAKLSRGGVPRNALYATTVIAGLCFLTSMFGNQTVYLWLLNTSGMTGFIAWLGIAIS
                     HYRFRRGYVLQGHDINDLPYRSGFFPLGPIFAFILCLIITLGQNYEAFLKDTIDWGGV
                     AATYIGIPLFLIIWFGYKLIKGTHFVRYSEMKFPQNDKK"
     gene            complement(60427..61308)
                     /locus_tag="NBI74_000265"
     CDS             complement(60427..61308)
                     /locus_tag="NBI74_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCD1930646.1"
                     /translation="MHITLRQLEVFAEVLKSGSTTQASVMLALSQSAVSAALTDLEGQ
                     LGVQLFDRVGKRLVVNEHGRLLYPRALALLEQAVEIEQLFREDNGAIRIYASSTIGNY
                     ILPAVIARYRHDYPQLPIELSVGNSQDVMQAVLDFRVDIGFIEGPCHSTEIISEPWLE
                     DELVVFAAPTSPLARGPVTLEQLAAAPWILRERGSGTREIVDYLLLSHLPKFEMAMEL
                     GNSEAIKHAVRHGLGISCLSRRVIEDQLQAGTLSEVAVPLPRLMRTLWRIHHRQKHLS
                     NALRRFLDYCDPANVPR"
     gene            61407..62456
                     /locus_tag="NBI74_000266"
     CDS             61407..62456
                     /locus_tag="NBI74_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family protein"
                     /protein_id="HCD1930647.1"
                     /translation="MTNITLQKQHRTLWHFIPGLALSAVITGVALWGGSIPAVAGAGF
                     SALTLAILLGMVLGNTIYPHIWKSCDGGVLFAKQYLLRLGIILYGFRLTFSQIADVGI
                     SGIIIDVLTLSSTFLLACFLGQKVFGLDKHTSWLIGAGSSICGAAAVLATEPVVKAEA
                     SKVTVAVATVVIFGTVAIFLYPAIYPLMSQWFSPETFGIYIGSTVHEVAQVVAAGHAI
                     SPDAENAAVISKMLRVMMLAPFLILLAARVKQLSGTNSGEKSKITIPWFAILFIVVAI
                     FNSFHLLPQSVVNMLVTLDTFLLAMAMAALGLTTHVSALKKAGAKPLLMALVLFAWLI
                     VGGGAINYVIQSVIA"
     gene            62530..63387
                     /gene="nfo"
                     /locus_tag="NBI74_000267"
     CDS             62530..63387
                     /gene="nfo"
                     /locus_tag="NBI74_000267"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HCD1930648.1"
                     /translation="MKYIGAHVSAAGGLANAAIRAAEIDATAFALFTKNQRQWRAAPL
                     TTQTIDEFKAACEKYHYTSAQILPHDSYLINLGHPVAEALEKSRDAFIDEMQRCEQLG
                     LSLLNFHPGSHLMQISEEDCLARIAESINIALDKTQGVTAVIENTAGQGSNLGFKFEH
                     LAAIIDGVEDKSRVGVCIDTCHAFAAGYDLRTPAECEKTFADFARIVGFKYLRGMHLN
                     DAKSTFGSRVDRHHSLGEGNIGHDAFRWIMQDDRFDGIPLILETINPDIWAEEIAWLK
                     AQQTEKAVA"
     gene            63391..64479
                     /locus_tag="NBI74_000268"
     CDS             63391..64479
                     /locus_tag="NBI74_000268"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HCD1930649.1"
                     /translation="MNDREKQILKILRRNPLIQQNEIADILQISRSRVAAHIMDLMRK
                     GLIKGKGYILTEQDYCVVVGAINMDIRGMADIRYPQAASHPGSVHCSAGGVGRNIAHN
                     LALLGRDVHLISAIGNDFYGETLLEETRRAGVNVSNCIRLHGHSTATYLAIANKQEET
                     ILAINDTHILQQLTPQLLNTSRDLIRHAGVVLADCNLTPEALEWVFTIADEIPMFVDT
                     VSEFKANKVKNWYSRIHTLKPTQNELEILWGQPIKDDNDRIRAVNSLHQQGVKRIFVY
                     LKDESVFCSDKDGEQFLLTAPAHTTVDSFGADDGFMAGLVYSFLEGYSFRDSARFAIA
                     CAAISRASGSLNNPTLSADNALSLVPMV"
     gene            complement(64747..65688)
                     /gene="rihB"
                     /locus_tag="NBI74_000269"
     CDS             complement(64747..65688)
                     /gene="rihB"
                     /locus_tag="NBI74_000269"
                     /EC_number="3.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosylpyrimidine nucleosidase"
                     /protein_id="HCD1930650.1"
                     /translation="MEKRKIILDCDPGHDDAIAIMMAAKHPAIDLLGITIVAGNQTLD
                     KTLINGLNVCQKLEINVPVYAGMPQPIMRQQIVADNIHGETGLDGPVFEPLTRQAENT
                     HAVKYIIDTLMASDGDITLVPVGPLSNIAVAMRMQPAILPKIREIVLMGGAYGTGNFT
                     PSAEFNIFADPEAARVVFTSGVPLVMMGLDLTNQTVCTPDVIARMERAGGPAGELFSD
                     IMNFTLKTQFENYGLAGGPVHDATCIGYLINPDGIKTQEMYVEVDVNSGPCYGRTVCD
                     ELGVLGKPANTKVGITIDTDWFWGLVEECVRGYIKTH"
     gene            65818..66516
                     /gene="yeiL"
                     /locus_tag="NBI74_000270"
     CDS             65818..66516
                     /gene="yeiL"
                     /locus_tag="NBI74_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311082.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator YeiL"
                     /protein_id="HCD1930651.1"
                     /translation="MKEIHNNDLKQQLMSESAFKDCFSTDVSADTRLFHFLARDYIVQ
                     EGQQPSWLFYLTRGRARLYVTLANGRVSLIDFFAAPCFIGEIELIDKDHEPRAVQAIE
                     ECWCLALPMKHYRPLLLNDTLFLRKLCVTLSHKNYRNIVSLTQNQSFPLVNRLAAFIL
                     LSQEGDLYHEKHTQAAEYLGVSYRHLLYVLAQFINDGLLIKSKKGYLIKNRKQLSGLA
                     LEMDPENKFSGMMQ"
     gene            complement(66583..67833)
                     /locus_tag="NBI74_000271"
     CDS             complement(66583..67833)
                     /locus_tag="NBI74_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HCD1930652.1"
                     /translation="MDIMRSVVGMVVLLAIAFVLSVNKKSISLRTVGAALLLQIAIGG
                     IMLYFPPGKWAVEQAALGVHKVMSYSDAGSAFIFGSLVGPKMDVLFDGAGFIFAFRVL
                     PAIIFVTALISLLYYIGVMGLLIRILGSIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRMNRNELFTAICSGMASIAGSMMIGYAGMGVPIDYLLAASLMAIPGGILFAR
                     ILSPATEPSQVTFENLSFSETPPKSIIEAAANGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGVGGWFGFANVSLESIFGYVLAPLAWIMGVDWSDANLAGSLIGQKLAINEFVA
                     YLNFSPYLQTSGTLDVKTIAIISFALCGFANFGSIGVVVGAFSAISPKRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(67927..68865)
                     /gene="psuG"
                     /locus_tag="NBI74_000272"
     CDS             complement(67927..68865)
                     /gene="psuG"
                     /locus_tag="NBI74_000272"
                     /EC_number="4.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HCD1930653.1"
                     /translation="MSELTLSPELLQISAEVQDALKNKKPVVALESTIISHGMPFPQN
                     AQTAIEVEETIRKQGAVPATIAIIGGVMKVGLSKEEIELLGREGHNVTKVSRRDLPFV
                     VAAGKNGATTVASTMIIAALAGIKVFATGGIGGVHRGAEHTFDISADLQELANTNVTV
                     VCAGAKSILDLGLTTEYLETFGVPLIGYQTKALPAFFCRTSPFDVSIRLDSAREIARA
                     MAVKWQTGLNGGLVVANPIPEQFAMPEETINAAIDQAVAEAEEQGVIGKESTPFLLAR
                     VAELTGGDSLKSNIQLVFNNAILASEIAKEYQRLVG"
     gene            complement(68853..69794)
                     /locus_tag="NBI74_000273"
     CDS             complement(68853..69794)
                     /locus_tag="NBI74_000273"
                     /EC_number="2.7.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine kinase"
                     /protein_id="HCD1930654.1"
                     /translation="MREKDYIVIIGSANIDVAGYSHHPLNYADSNPGKIKFTPGGVGR
                     NIAHNLALLGKNAWLLSAVGGDFYGQSLLAQTNQSGVYVDKCLIVPGENTSSYLSLLD
                     NTGEMLVAINDMSISDCISAEFLAQHQEFIRGAKVIVADCNLSEEALVWVLENSGETP
                     VFIDPVSAWKCVKIRDHLSKIHTLKPNRLEAETLSGIALSGREDVAKVAAWFHQHGLN
                     RLVLSMGGDGVYYSDINGESGWSAPIKTNVINVTGAGDAMMAGLASCWVDGMPFIDSV
                     RFAQGCSSMALACEYTNNPELSIANVTSLVENTECLN"
     gene            complement(70250..71941)
                     /gene="fruA"
                     /locus_tag="NBI74_000274"
     CDS             complement(70250..71941)
                     /gene="fruA"
                     /locus_tag="NBI74_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIBC"
                     /protein_id="HCD1930655.1"
                     /translation="MKTLLIIDANLGQARAYMAKTLLGAAARKAKLEIIDNPNDAEMA
                     IVLGDFIPNDSALNGKNVWLGDISRAVAHPELFLSEAKGHAKPYTAPVAATAPVAASG
                     PKRVVAVTACPTGVAHTFMAAEAIETEAKKRGWWVKVETRGSVGAGNAITPEEVAAAD
                     LVIVAADIEVDLAKFAGKPMYRTSTGLALKKTAQELDKAVAEATPYEPAGKAQTATTE
                     GKKESAGAYRHLLTGVSYMLPMVVAGGLCIALSFAFGIEAFKEPGTLAAALMQIGGGS
                     AFALMVPVLAGYIAFSIADRPGLTPGLIGGMLAVSTGSGFIGGIIAGFLAGYIAKLIS
                     TQLKLPQSMEALKPILIIPLISSLVVGLAMIYLIGKPVAGILDGLTHWLQTMGTANAV
                     LLGAILGGMMCTDMGGPVNKAAYAFGVGLLSTQTYGPMAAIMAAGMVPPLAMGLATMV
                     ARRKFDKAQQEGGKAALVLGLCFISEGAIPFAARDPMRVLPCCIVGGALTGAISMAIG
                     AKLMAPHGGLFVLLIPGAITPVLGYLVAIIAGTLVAGLAYAFLKRPETQIVEKNA"
     gene            complement(71958..72896)
                     /gene="fruK"
                     /locus_tag="NBI74_000275"
     CDS             complement(71958..72896)
                     /gene="fruK"
                     /locus_tag="NBI74_000275"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HCD1930656.1"
                     /translation="MSRRVATITLNPAYDLVGFCPEIERGEVNLVKTTGLHAAGKGIN
                     VAKVLKDLGIDVTVGGFLGKDNQDGFQQLFSELGIANRFQVVQGRTRINVKLTEKDGE
                     VTDFNFSGFEVTPADWERFVTDSLSWLGQFDMVCVSGSLPSGVSPEAFTDWMTRLRSQ
                     CPCIIFDSSREALVAGLKAAPWLVKPNRRELEIWAGRKLPEMKDVIEAAHALREQGIA
                     HVVISLGAEGALWVNASGEWIAKPPSVDVVSTVGAGDSMVGGLIYGLLMRESSEHTLR
                     LATAVAALAVSQSNVGITDRPQLAAMMARVDLQPFN"
     gene            complement(72896..74026)
                     /gene="fruB"
                     /locus_tag="NBI74_000276"
     CDS             complement(72896..74026)
                     /gene="fruB"
                     /locus_tag="NBI74_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused PTS fructose transporter subunit IIA/HPr
                     protein"
                     /protein_id="HCD1930657.1"
                     /translation="MFQLSVQDIHPGEKAGDKEEAIRQVAAALVQAGNVAEGYVNGML
                     AREQQTSTFLGNGIAIPHGTTDTRDQVLKTGVQVFQFPEGVTWGDGQVAYVAIGIAAS
                     SDEHLGLLRQLTHVLSDDSVAEQLKSATTAEELRALLMGEKQSEQLKLDNEMLTLDIV
                     ASDLLTLQALNAARLKEAGAVDATFVTKAINEQPLNLGQGIWLSDSAEGNLRSAIAVS
                     RAANAFDVDGETAAMLVSVAMNDDQPIAVLKRLADLLLDNKADRLLKADAATLLALLT
                     SDDAPTDDVLSAEFVVRNEHGLHARPGTMLVNTIKQFNSDITVTNLDGTGKPANGRSL
                     MKVVALGVKKGHRLRFTAQGADAEQALKAIGDAIAAGLGEGA"
     gene            74394..75575
                     /gene="setB"
                     /locus_tag="NBI74_000277"
     CDS             74394..75575
                     /gene="setB"
                     /locus_tag="NBI74_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetB"
                     /protein_id="HCD1930658.1"
                     /translation="MHNSPTVTSAKSFDLTSTAFLIVAFLTGIAGALQTPTLSIFLTD
                     EVHARPAMVGFFFTGSAVIGILVSQFLAGRSDKRGDRKSLIVFCCLLGVLACTLFAWN
                     RNYFVLLFVGVFLSSFGSTANPQMFALAREHADKTGREAVMFSSFLRAQVSLAWVIGP
                     PLAYALAMGFSFTVMYLSAAVAFIVCGVMVWLFLPSMQKELPLATGTVEAPRRNRRDT
                     LLLFVICTLMWGSNSLYIINMPLFIINELHLPEKLAGVMMGTAAGLEIPTMLIAGYFA
                     KRLGKRFLMRVAAVGGVCFYAGMLMAHSPVILLGLQLLNAIFIGILGGIGMLYFQDLM
                     PGQAGSATTLYTNTSRVGWIIAGSVAGIVAEIWNYHAVFWFAMVMIIATLFCLLRIKD
                     V"
     gene            complement(75572..75826)
                     /locus_tag="NBI74_000278"
     CDS             complement(75572..75826)
                     /locus_tag="NBI74_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HCD1930659.1"
                     /translation="MECRPGCGACCTAPSISSPIPGMPDGKPANTPCIQLDEQQRCKI
                     FTSPLRPKVCAGLQASAEMCGNSRQQAMIWLIDLEMLTAP"
     gene            75981..76553
                     /gene="yeiP"
                     /locus_tag="NBI74_000279"
     CDS             75981..76553
                     /gene="yeiP"
                     /locus_tag="NBI74_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007750416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P-like protein YeiP"
                     /protein_id="HCD1930660.1"
                     /translation="MPRANEIKKGMVLNYNGKLLLVKDIDIQSPTARGAATLYKMRFS
                     DVRTGLKVEERFKGDDIVDTVTLTRRYVDFSYVDGNEYVFMDKEDYTPYTFTKDQIEE
                     ELLFMPEGGMPDMQVLTWDGQLLALELPQTVDLEIVETAPGIKGASASARNKPATLST
                     GLVIQVPEYLSPGEKIRIHIEERRYMGRAD"
     gene            76776..78242
                     /locus_tag="NBI74_000280"
     CDS             76776..78242
                     /locus_tag="NBI74_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HCD1930661.1"
                     /translation="MNTIASVTLPHHVHAPRYDRQQLQSRIVHFGFGAFHRAHQALLT
                     DRVLNAQGGDWGICEISLFSGDQLMSQLRAQNHLYTVLEKGADGNQAIIVGAVHECLN
                     AKLDSLAAIIEKFCEPQVAIVSLTITEKGYCIDPATGALDTSNPRIIHDLQNPEEPHS
                     APGILVEALKRRRERGHTPFTVLSCDNIPDNGHVVKNAVLGMAEKRSPELAGWIKEHV
                     SFPGTMVDRIVPAATNESLAEISQHLGVNDPCAISCEPFIQWVVEDNFVAGRPAWEVA
                     GVQMVNDVLPWEEMKLRMLNGSHSFLAYLGYLSGFAHISDCMQDRAFRHAARTLMLDE
                     QAPTLRIKDVDLTQYADKLIARFANPALKHKTWQIAMDGSQKLPQRMLAGIRIHLGRE
                     TDWSLLALGVAGWMRYVSGVDDAGNAIDVRDPLSDKIRELVAGSSSEQRVTALLSLRE
                     IFGDDLPDNPHFVQVIEQAWQQIAQFGAHQALLNTLKI"
     gene            78360..79346
                     /gene="yeiR"
                     /locus_tag="NBI74_000281"
     CDS             78360..79346
                     /gene="yeiR"
                     /locus_tag="NBI74_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding GTPase YeiR"
                     /protein_id="HCD1930662.1"
                     /translation="MTRTNLITGFLGSGKTTSILHLLAHKDPNEKWAVLVNEFGEVGI
                     DGALLADSGALLKEIPGGCMCCVNGLPMQVGLNTLLRQGKPDRLLIEPTGLGHPKQIL
                     DLLTAPVYEPWIDLRATLCILDPRLLLDEKSASNENFRDQLAAADIIVANKSDRATPE
                     SEQALERWWQQNGGDRQLIHSEHGKIDGHLLDLPRRNLAELPASAAHSHQHSVKKGLA
                     ALSLPEHQRWRRSLNSGQGHQACGWIFDADTVFDTIGILEWARLAPVERVKGVLRIPE
                     GLVRINRQGDDLHIETQNVAPPDSRIELISSSEADWNALQSALLKLRLATTA"
BASE COUNT        19695 a        20902 c        19721 g        19125 t
ORIGIN      
        1 ttctaaatcc gattaaaacc agggtggcaa attaatcgta gcgatttaac acgattcgac
       61 gtttgctatc gctatctgca gcaatcactt ccgtcgttac acctttaact ggcgaagaaa
      121 cctgatgacg ggtatcaaca ttatacgtta actgaccaca atgctgttga ggttgaccat
      181 taaccatagc catactgcat gtcgggggac tgatcctacc attaatcgtt aatgtggcag
      241 tttctgaaga gaacgcggga gaagccaatc cataggcaag aaaaataatt cctgacagcc
      301 agtacttatt cattgaacgt tatccctgta gtaaaggtta tgcctggaaa gaatgagtgc
      361 ataacaaact atagctgtac atccactaca cagccacgaa ggatgataat gaagcatcgc
      421 cggtacgatc aatcgacttt gtagagtttc ggacgaaggt ccgcagaata ttcgcaacat
      481 taaataagtg ttcattaatg ttgcaaaaac aaatacaccg catcaatgta acatctctgc
      541 ggtgtatgaa gaataaccag aatggttaat acgcgttaga ccgcgcggaa ggaaatctcg
      601 ccaggaatga cttcaccctg ccagtagagc tgagctgcaa cgcggtcagc cagctggcgg
      661 tagatagcgg taaattcgct ctcaggacgg ctgataaccg ttggcgtgcc tttatcgaga
      721 tcctcacgta aggagatgtg taacggcatc tgccccagca gctgcgtgtg gtatttctcg
      781 gccagtttct ctgcaccacc ggtaccgaaa atcggctcat ggtgaccaca gttactgcaa
      841 atatgcacac tcatgttctc gacaataccg agcaccggaa cctcgacttt ctcgaacatc
      901 acaatgcctt ttttcgcatc gatcaacgcg atgtcctgcg gggtggtgac cactaccgcg
      961 ccagttactg gaatgttttg tgccagtgtc aattgaatgt caccggtacc tggcggcata
     1021 tcaagcacca gatagtcgag atccggccac aaggtttcct gcaacatttg cattaacgcc
     1081 ttgctcgcca ttgggccacg ccacaccatc gcattgtcgt cagtgaccag ataaccaatt
     1141 gaattggttg ccaggccatg agacataatc ggtgccatat gggtaccgtc cggcgaagtt
     1201 ggacgctgat tttctgcgcc cagcatggta gggattgatg ggccgtagat atcggcgtcc
     1261 agaatgccga ctttcgcccc ttctgccgcc agtgccaacg ccaggtttac cgccgtggag
     1321 gatttaccca cgccaccttt accggaactg accgcgataa tgttcttcac gccgttaatt
     1381 cccggctgat tcttcacgcg tttcagagtg gctatgttat gcgacagttt ccagtcgata
     1441 gctttcgctc cggtgatgcg cagcagttcg gcgctacatt gctcttttaa ctcttcgaac
     1501 gcgctatgcc agacgaatgg catgaccagt tccacatgca gcgtgtcgtc catccaggcg
     1561 acatggtgca acgctttcag cgttgtgagg ttatgcttca gggttgggtg ctgaaaattg
     1621 gccagtgtcc cggcgaccat tgcgcgcaag gcttctggtg atttggcctg ggattgttcg
     1681 ttcatcccga ctcctttttt gtatagataa accatcagct gatagtttac ctgaagaata
     1741 tagagaagta cttacttaac attttcccat ttggtactat ctaagccctt ttcactatta
     1801 agaagtaatg cctactatga ctcaagtcgc gaagaaaatt ctggtgacgt gcgcactgcc
     1861 gtacgctaac ggctcaatcc acctcggcca tatgctggag cacatccagg ctgatgtctg
     1921 ggtccgttac cagcgaatgc gcggccacga ggtcaacttc atctgcgccg acgatgccca
     1981 cggcacgccg atcatgctta aagctcagca gcttggtatc accccggagc agatgattgg
     2041 cgaaatgagt caggaacatc agactgattt cgcaggcttt aacatcagct atgacaacta
     2101 tcactcgacg cacagcgaag agaatcgcca gttgtcagaa cttatctact ctcgtctgaa
     2161 agaaaacggt tttattaaaa accgcaccat ctctcagctg tacgatccgg aaaaaggcat
     2221 gttcctgccg gaccgttttg taaaaggcac ctgcccgaaa tgtaaatccc cggatcaata
     2281 cggcgataac tgcgaagtct gcggcgcgac ctacagcccg actgaactga tcgagccgaa
     2341 atcggtggtt tctggtgcta ccccggtaat gcgtgattct gaacacttct tctttgatct
     2401 gccctctttc agcgaaatgc tgcaggcatg gacccgcagc ggtgcgttgc aggagcaggt
     2461 ggcaaataaa atgcaggagt ggtttgaatc tggcctgcaa cagtgggata tctcccgcga
     2521 cgcgccttat ttcggttttg aaattccgaa cgcgccgggc aaatatttct atgtctggct
     2581 ggacgcaccg attggctata tgggctcttt caagaatctg tgtgacaagc gcggcgacag
     2641 cgtaagcttc gatgaatact ggaagaaaga ttccactgcc gagctgtatc acttcatcgg
     2701 taaagatatc gtttacttcc acagcctgtt ctggcctgcc atgctggaag gcagcaactt
     2761 ccgcaagccg accaacctgt ttgttcacgg ctatgtgacg gtgaacggcg cgaaaatgtc
     2821 caagtctcgc ggcaccttta ttaaagccag cacctggctg aatcattttg acgccgacag
     2881 cctgcgctac tactacaccg ccaaactgtc atcgcgcatt gatgatatcg atctcaacct
     2941 ggaagatttc gttcagcgcg tgaatgccga tatcgtgaac aaagtggtta acctggcgtc
     3001 ccgtaacgct ggctttatca ataagcgttt tgacggtgtg ctggcaagcg agctggctga
     3061 cccgcagttg tacaaaacct tcactgatgc cgctgaagtg attggcgaag cgtgggaaag
     3121 ccgtgaattt ggtaaagcta tccgtgaaat catggcgctg gctgacctgg ctaaccgcta
     3181 tgtcgatgaa caggctccgt gggtggtggc gaagcaggaa ggtcgcgatg ccgatctgca
     3241 ggcgatttgc tcaatgggca tcaacctgtt ccgcgtgctg atgacttacc tgaaaccggt
     3301 actgccgaaa ctgaccgagc gcgcagaagc attcctcaat acagaactga cctgggatgg
     3361 tatccagcaa ccgctgttgg gccataaagt gaatccattc aaggcgctgt ataaccgcat
     3421 cgatatgaaa caggttgaag cactggtgga agcatccaaa gaggaagtaa aagccgctgc
     3481 cgcgccagta actggcccac tggcagatga tccgattcag gaaaccatca cctttgacga
     3541 ctttgctaaa gttgacctgc gcgtggcgct gattgaaaac gcagagtttg ttgaaggttc
     3601 tgacaaactg ctgcgcctga cgctggatct cggcggtgaa aaacgcaatg tcttctccgg
     3661 cattcgttct gcttacccgg atccgcaagc actaattggt cgtcacacca ttatggtggc
     3721 taacctggca ccacgtaaaa tgcgcttcgg tatctccgaa ggcatggtaa tggctgctgg
     3781 tcctggtggg aaagatatct tcctgctaag cccggatgcc ggtgctaaac cgggtcatca
     3841 ggtgaaataa tcccccttca aggcgctgca tcgacagcgc cttttcttta taaattccta
     3901 aagttgttct cttgcgattt tgtctctctc taacccgcat aaatactggt agcatctgca
     3961 ttcaactgga taaaattaca gggatgcaga atgagacact ttatctatca ggacgaaaaa
     4021 tcacataaat tctgggcggt ggagcagcaa ggcaacgagt tacatgtcag ttggggcaaa
     4081 gtcggcacta acgggcaaaa ccaggtaaaa agctttgctg atgctgcggc ggcggaaaaa
     4141 gcaaagctta agctgatcgc agagaaaacg aaaaaaggct atgtggaaaa tgcttctgca
     4201 aacgtgcata tcacccccat caccaaagcc actcctgaag ttgaaacttc tcctgagagt
     4261 aaaaaccaac gcccctggct ggctgatgat gcggtcattc ggctaactga cgatattaat
     4321 cgatttgctt ttcctcatcg ctctcgcccc agagagatca attatctgca caaagatagt
     4381 caaatatggc agcgcatcgc gaataacacc agggcatatg atcccgccaa caattaccgt
     4441 tcttatccag aaaactggca gcaggctttt gctgagttac aaaggcgaat tctggataat
     4501 caacagacag ggagtgcgca atctgatgcg gcattgctct ggagtttctg gaattcttac
     4561 tctgcagatg aactagtcga tgacttaatc attcgctgtg gtatggaaag tgccgttgaa
     4621 attgcccttc tcgctcttca actgagatat aaaccagtaa aaggcgcagt aaccaccatc
     4681 attccaccta atcacgaagc agaatcgcta ccgagttggc atcaacgcct atgtcatcat
     4741 ctttcactcg cttcagaaga tgagtggcaa cgctgtgtag ataaagtact cgcggctatt
     4801 acgacgctat ccccagcacg gcaacctttt gcggcgttac tgctccctga acgcccggat
     4861 attgccaatg ctatggctct acgctatgca gatcaaaacg ttccggcgat gacctggtta
     4921 agactggtag taaccgatga agccgctctg actgcagccg aaaaatacga ctttccaccg
     4981 ttatatcgtg attttcgcgc ttatctggca atgttgctgg caaataatgg ggtgagcggt
     5041 gtaagccgga tacttactga gtttgtggaa gagcattctg acaatcccac ttatctcttc
     5101 gagcgcatca gcgagactga agatcttgtc aaatggttat ggaaaacgaa tcacccggat
     5161 gcgattcaaa ttctgatcct cggtgtaatt ggcaagaaaa agcacctgga atacttaagc
     5221 aaagcatgtc aaaaacatcc cgctgcggct attgccgctt atgcaacttt gctggcaata
     5281 catgaagata aagagtggca taaagcgctt gtcaaattga ttaccatcac gccagagtta
     5341 gtatgcgacg tcattccctg ggttagtgct aaagcggcag gcatcttatc tgaatgtcgt
     5401 cctcagtcag tagctgaaga atgtgagtat gccactgcgg atatgttgcc agagctgttt
     5461 gtttcgccgc cctggatgac taaagagaaa aagaaaaatt cgccggtgtt tgatcttcct
     5521 gtactgccag ttccttctgt ttctgacgtc actccagaga ttgccaaaaa actaacccgt
     5581 acataccttg tcacccgctt ccagcaaata gcccaacagc aggcaacaaa acaaatattg
     5641 tttaccgacc tgccgccgat ggaaaaagca agctgggaga gacacttagt ccccctcaca
     5701 ccagaacagc aaattctgtg gtgtctgggt tttgacaaat ggcgggagag cggagaaaaa
     5761 atatacgaaa aaattccggc gccacaaagt gccgttgatg ccctgctacg cttcgatttt
     5821 ccagcactca atgctgaatt tgcgcaatac cataacaatg cttacaaaag ctggaatctc
     5881 attgccctct gttatctacc tggtcaacag gccatttctt ttcttaacca gatagtcaaa
     5941 gaagataact actctggtga gggatatatt ctggccattt ttggtagcgc cgccattcct
     6001 gcatttatga cctgcctgca acgtgagcca caacggttgt ggtttttcac cttcttcctt
     6061 ggtgtcaatg aactggcatt gccaatggcg caacgacttc agaaaaaaat gtcgtatgaa
     6121 gatgcacgaa actggctaac ggattaccca cgccatgccg ccgccgggct acttcctgtg
     6181 gcactgggca aacaaggaaa agatcgtgat tgtgcccgcc aggcgctccg tctgctggtg
     6241 aatttaaacc agcgagaaac gattgaggaa atcgcccaag gatacaacca acctgatgtt
     6301 ttagctgcgc tggcaacatt atttgatagc gagccgctgg aagaatatcc cgctaaaatc
     6361 gccccactgc ccggctttta tcagttcacc ttatggcgca gaccacggct taaaagtaac
     6421 aaccagcctc tgccagatga cgctatgcgc cacctcggca taatgctgag ctttccacgc
     6481 gacattaccc cctacgcagg gcttgcgatc atcaaagaaa ccttcacccg cgaatcactg
     6541 gctgaatttg gctgggatct gtataccgcc tggacagaag ctggcgcacc cgcaaaagaa
     6601 aactgggcat ttacctcgct ggggatttta ggcaatgacg acaccgcgcg taaattaacc
     6661 ccgctcatcc gcacctggcc gggtgaatcc cagcataaac gcgcggttta cgggctggat
     6721 gtgttagcca gtatcggtag cgacattgcg ctaatgttgc ttaatggcat tgcgaaaaag
     6781 attaaattcg ttgcattaca ggaacatgcc tgcgacaaaa tcaacatggt cgctgaaaat
     6841 cgcgggctga ctatggctga actggaagac cgcctggcac cggatttagg acttgatagc
     6901 agtggctcgt tgacactgga tttcggccca cgtcaattta ccgttggttt tgacgaaact
     6961 ttaaaacccg tggttcgcga cgcaaacggc aaagttctga aagatttacc caaaccaaat
     7021 cagagcgatg ataaaacgct ggcaactgac gcggttaatc tcttcaaaca gttgaaaaaa
     7081 gatgtacgcg ccatagccag ccagcagatt gatcgtctgg agcaggctat gtgccagcgc
     7141 agacgctgga cggcagagca gttccgcctg ttcctggtgg agcatccgct ggtacgtcac
     7201 ttaacccgac ggttgttatg gggggtatat actgaggaaa acacgctgat tgcctgcttc
     7261 cgggtcgcag aagacagcac ttacagcgat gcgcaggatg agctatttac gttgccagca
     7321 ggaaacatcg gtattccgca tgtgctggaa atatctcctg aatccgccgc tgcatttggg
     7381 caaatttata ctgattacga acagcttcct ccgttccgcc aactcgatcg tggttattac
     7441 caccttgctg ataatgaacg tgattcccat gaattgatac gctggcaagg acgtctttgc
     7501 caggccgggc gcattgtcgg gctggaacgc cgaggctggc aacgtctgga agaaagcggc
     7561 agcgtttacg cgatgcgcaa atctactcct tatggcgatc tcgaacttga aacagaacct
     7621 ttttcattaa tttatggtga aacggggtat ggcgaccagc taccggttga aagcgtaaaa
     7681 atcacctcgc ctgataatcg ctacggtaaa caatcctcac tcactttttc cgcgctcgac
     7741 gacatcaccg ccagcgaact cattaacgac attgaatcac tgtttgatta aaaggattta
     7801 gcaatgaaaa cctatattta tcaggacgaa aaatcacata aattctgggc agtagagcag
     7861 caggaaaatg aactgcatat cagttggggc aaagtcggca ctaacgggca aagccagata
     7921 aaaagctttg ctgatgcggc ggcggcggaa aaagcggagc tgaagttgat tgcggagaaa
     7981 acgaaaaagg gctacgcaga agaagtatcc gtgacgacac cgacatctgc ccccgtccag
     8041 gttatcgcat gctctgaaaa agcccctttg ccgcaagaca aaccaccatt tgctgaagac
     8101 caccttccct ggctggcaga cgacgcccaa atcattttac cgacagaggt cgcaccaacc
     8161 acactaagtc atcgacgctg gcctggcgat tctgtgccac aggagaatga attaactctg
     8221 ctacgctccg tcgccgcgca tactcaccgg cgttttaaaa aggtgatcac cttcgattac
     8281 agcacctgtt cactggactg gcagcaggcg attacacaag cagtgggcct tatcgactca
     8341 ccgattagca ccgcactacc accgatggtt ctggcggtac tggttgcgct ggaacaaggc
     8401 ttcaatcgca acgatcacga agagttaatg gatcaaattg tccaggaggg tggtctggaa
     8461 tatgccactg aggttgtcat tgcattgcaa gccatccgct ttgactggaa ttatgatgcc
     8521 cacctcatta cgtttacccc ggataacaag cagcctggct atctgtcacg ctttgcctcg
     8581 gttgaaatgc gtctgcgtaa acatctctca ctagcaaatg acgatgtatg gcagcgctgc
     8641 gccgacaaac tgattgcagc tctcggaaac atacccgcct ggcatcagcc gctggttgcg
     8701 ttattgttgc cggaaaaaca agatgtgagc catgagattg ccagacgtct ttgcggacaa
     8761 aaagggttat atgcgctgga atggctaaaa ctaacggccg ccgacgagca ggtattggca
     8821 gaactgggga aatactatcc aggacagcca gggcaggtat ttgatgatta ttacggcggt
     8881 aaaatctggt gcgcaacgat actcaaggag caaggtgtcg gtgcgctggc ccgttttgct
     8941 ccttacgctg ctggtgacac atgtggtgaa gtgttgatgc atatcaacca cccgcaggca
     9001 cttacactac ttatccatgc aagcgagcag ggaaagcgtt gccacgatcg catgacaaag
     9061 actttcgttc gctttccaca tgcagcgctg gcggcattag cagaacttct ggcacaaaaa
     9121 gatcaaaagc gttggcgcat gatgctaatg acaatgctta tctcacaacc aacattagca
     9181 gagcgagtaa tcccctggct ctcaacaccc gcagttgccg tactgaaatc atgccagcaa
     9241 caactgacac agccctcaaa ccatgccagc gctgatatgc tgcctgccgt attagtctca
     9301 ccgccctggc tttcgaaaaa gaaaaaatct gtaatgccgg tattggattt aacgccatta
     9361 ccgctggagt catgctgcac attgaccgag acggcagaaa aagagattca tgcaaggcat
     9421 cgctggcacg cacatcaaat agacatcggc caaaaagagg atattcagaa ctacctgaca
     9481 cgtctgggtt tcaatcgctg gaataatggc cagtatatga aagcgtctga tgctgttgta
     9541 gaactctggc agaggggaga ttattctgcg ctaatcagcg aatttaaaac cttctggcac
     9601 tcctatcaac gggaatggca gctttacatg ttagccgccc tgcctatcga aaaaacagcg
     9661 caggcgtgga atgtcctcag taaagagcca cacgtcggtg tcgagtttgt catgacgcac
     9721 ctgcaactcg ctgggttaca gggttttatt cactcctttt cacgctatcc acaagaagct
     9781 ctgcctgtag cacaatattt tgcggctatc gaattagcgc cactgatcgc ccgcgccttc
     9841 aacaaactca aaacattacg acaagacgcc cgtatctggc tgctgaaata cccggaacac
     9901 gccattaccg gcctgctgcc tgcagcgctc ggcaaaaccg gtgaagcaca ggacaacgcc
     9961 cgtgctgcct tgcgtatgct taccgaaaac ggtcatcagc cattgctgca agaaatcgcc
    10021 cggcgttata accagccgga agtaaccgat gcggtgaacg ctctacttgc gctcgatccc
    10081 ttagataatc acccgacaaa aatccccact cttccgacct tttatcagcc atcgctctgg
    10141 acgcgcccgt tattaaaagc aaacgcccaa tcactgcctg atagcgcact cctccacctc
    10201 ggtgaaatgc tccgcttccc tcaggaagag gcactgtatc caggattatt gcaggtgaaa
    10261 gatgcctgta ccaccgactc actggctgaa tttgcctggg atctgtttac cgcctggcag
    10321 accgctggcg cgccgtcgaa agagagttgg gcgttcactg cgttaggcgt tctcggtaac
    10381 gatgacaccg cccgcaaact gacgccatta atccgtgcct ggcctggcga atcccagcat
    10441 aaacgtgcca ccgttgggtt ggatatcctc gccgctatcg gtagtgatat cgccctgatg
    10501 cagcttaacg gcatcgccca gaaactgaaa ttcaaagcat tacaggagcg ggcaaaagaa
    10561 aaaatagcca acattgccga gagccgcgaa ctcacggtgg cggagcttga agatcggtta
    10621 gcaccggatc tcggtctgga tgataacggt tcgttgctgc tggatttcgg cccacgccag
    10681 ttcaccgtca gctttgatga aaccttaaaa ccgtttgtgc gtgacgcttc cggcagtcgt
    10741 ctgaaagacc tgcccaaacc aaacaaaagc gatgatgaat cacaggcgaa caatgctgtt
    10801 aaccgctaca aattgctgaa aaaagatgcg cgtaccgtcg ccgcccagca ggtagcaagg
    10861 ctggaatccg ccatgtgcct gcgtcgccgc tggtcaccgg aaaactttca gctcttcctg
    10921 gttgagcatc cgctggtccg ccacttaacc cgccgtctga tttggggcgt ttatagcgcc
    10981 gaaaaccagc tactggcttg ctttcgcgta gcggaagata acagctacag caccgctgac
    11041 gatgatcttt tcaccctgcc ggaaggcgat atctctattg gtattcctca cgttctggaa
    11101 atatcaccga cggatgctgc cgccttcggg cagctttttg ccgactacga actgttacca
    11161 ccgttccgcc agcttgatcg taacagttac gcgctgacag aagccgagcg caatgccagc
    11221 gaactgacac gctgggcagg cagaaaatgc ccgagtggtc gggtaatggg gctggcaaat
    11281 aaaggctgga tgcgtggtga accgcaggac ggtggctgga tcggctggat gatcaaacct
    11341 ctgggccgct ggtcgttaat catggaaatc gatgaaggtt ttgcggtcgg catgtcgcca
    11401 gccgaactca gtgccgagca actcttaagc aagctgtggc tatgggaagg caaagcggaa
    11461 agctatggct gggggagtaa ttcaacacag gaagcgcagt tctccgtact cgatgccatc
    11521 accgccagcg agctaattaa cgatattgaa gccctgtttg aataaggaaa gagcatggac
    11581 aaggaattac cgtggctggc ggataacgcc caactggaac tgaaatataa aaaaggcaaa
    11641 acaccactca gccatcgcaa ttggccaggc gaaccggtac ctgttatcac tgaaaacatc
    11701 attcagacac tgggtgatga attgctacaa aaagcagaga agaaaaaaaa cattgtctgg
    11761 cgttatgaaa acttttcact ggagtggcag tccgccatca cgcaggccat caacttgatc
    11821 ggcgaacaca aaccctcaat cacggcccgg acaatggcgg cgctagcctg tatcgcgcaa
    11881 aatgatagcc aacagttgct cgacgaaatc gtccaacaag aggggctgga atatgcaact
    11941 gaggtggtga ttgcacgcca gtttattgta cggtgttatg agagtgatcc tctggtagta
    12001 acattgcaat atcagaacga ggactatggc tatggttatc gctcagaaac ctataacgaa
    12061 ttcgatctcc gattgcgtaa gcatctctct ctggcagagg aaagctgctg gcagcgttgc
    12121 gccgacaaac tcattgccgc actgccagga ataaccaaag ttcgccgccc ttttattgcg
    12181 ctgaacctcc cggaaaaacc cgaaatcgcc aacgagctgg taagccttga atgttcgcaa
    12241 actcattttc gctcaaaaga gtggttaaaa gttgttgctg atgaccctaa ggcggtgaaa
    12301 gaactcgcac gctactggag ccaggatata tttagcgatc gagaagccag ctacatgtcg
    12361 catgaaaacc acttcggcta cgcggcctgc gctgcccttt tgcgcgaaca aggactggca
    12421 gccgttccgc gcctcgcgat gtatgcacat aaagaagatt gcggcagtct gctggtacaa
    12481 attaaccatc cgcaagtcat ccgctccttg ctactggtgg ctgataaaaa caaacccagc
    12541 ctgcaacgtg tggctaaata cagtaaaaac ttcccccatg cgacgctcgc cgcactggca
    12601 gagctgctgg cgttaaaagc cccccctgcg cgccctggtt atcccatcat cgaagacaaa
    12661 aagctgcctg cacagcaaaa agcacgtgat gaatactggc atacgctgtt acaaacgctg
    12721 atggcatcgc agccacaact ggcagaagag gtgatgccgt gcttatgtac acaagcccag
    12781 gcagtggtga atggttattt atcggcatcg ccaaaacttg ctttcgagag tactcatagc
    12841 aacgacctgc cagaaatact tgtttctcca ccgtggcgag gtaagaaaaa aacggcattg
    12901 ctgcgccttg atttagtgcc tcttgaatta gcgcccaaag cccgctggca accaggcgaa
    12961 cgagagagac ttgccgccac agagtctgcc cgttatttca gcacgggatc ttttacggaa
    13021 cgcatggaac gaaaaagtgg tcgagttgta ctacaggagc taggttttgg ggatgatgta
    13081 tggctgttcc ggaattatat actgcccggc aaactggatg cggcgcgcaa atcattagtg
    13141 ggtcagtggc attactcccc gcgcagagta gaggaaatca acaatggctg gcactccacg
    13201 gaagcaaaat cagccgaaca ggcgcttcgt tccggcgacg ttgaagcgtt aattaacacc
    13261 tgggaaaatg atagctactc gcattatcgt caggaaaaga gtgtctggaa cctgtattta
    13321 ttggcgcagt tgccacgtga gatggctttg actttctggc tgcgtatcaa tgagaaaaag
    13381 catctattcg cgggtgagga ctattttctc agtatcctcg ggttggatac attacctggc
    13441 ctgttattgg ctttttcaca tcatccaaaa gaaacatttc cgttaatttt aaatttcggc
    13501 gcaacagaac tggccctgcc cgttgcccgc gtctggcgac gtttcgcggc gcagcgtgat
    13561 ctggctcgcc agtggatttt acaatggccg gaacatacgg ctactgcact tatccctctt
    13621 gtctttacaa aacccagcga taatagcgaa gctgcattac ttgccctgcg tttactgtat
    13681 gaacaagggc atggcgaatt actgcaaacc gtggcaaacc gctggcagcg tacagatgta
    13741 tggcctgccc tggagcaatt acttaaactg ggtccaatag aaatttaccc ggcacgtatt
    13801 ccaaaagctc ctgatttctg gcatccggga atgtggtcca ggccgcgcct tatcactaat
    13861 aatcagcctg ttaccgatga cgctctggaa attatcggcg aaatgctgcg ctttacccag
    13921 ggcggacgtt tttatggcgg actggaacag ctgaaaacgt tttgccagcc gcaaacgctg
    13981 gcagcttttg cctgggatct tttcaccgcg tggcaacaag ctggtgcccc cgcaaaagac
    14041 aactgggcat ttctggcgtt aagtctcttt ggtgacgaaa ccacggcacg agatctgacg
    14101 acacagatcc tcgcctggcc acaagaaggc aaatctgccc gtgctgtcag tggcctgaac
    14161 atccttaccc tgatgaataa tgatatggcg ctgatacagc tgcaccatat atcccaacgg
    14221 gcgaaatccc gctccttacg tgataacgcg gcggaatttc ttcaggtggt cgcagaaaat
    14281 cgcgggctaa gccaggaaga gttagcggac agattagtcc caacattggg ccttgatgat
    14341 ccgcaggcgt tgagttttga ttttggtccc cggcagttta ccgttcgctt cgatgaaaac
    14401 ctcaacccgg ttatctttga tcagcaaaac gttcgccaga aaagtgttcc ccggttgcgc
    14461 gccgatgacg atcaactgaa agcgcccgag gcactggccc gactaaaagg gctaaaaaaa
    14521 gatgccactc aggtgagcaa aaacctgctc ccacgtcttg aagcagccct acgtatcacc
    14581 cgccgctggt cgctggcaga ttttcattct ctgtttgtta atcatccctt tacccgcctg
    14641 gttacccagc gattaatatg gggggtttat ccggcaaatg aaccgcgtcg tttactcaac
    14701 gcctttcgtg tggccgcaga gggggagttc tgcaatgcac aagatgagcc cattgacctg
    14761 cctgcggatg ctctgattgg cattgcccac ccgttagaaa tggcagcaga aatgcgcagt
    14821 gaatttgcac agctttttgc cgattacgaa attatgccgc cttttcgcca gttggcgcgg
    14881 cgcacggtgc tgctcacacc tgacgagtca gccagtaaca gcctgactcg ctgggaaggt
    14941 aaatccgcta ccgttgggca acttatggga atgcgataca aaggctggga gtcaggctat
    15001 gaggacgcat ttgtctatga cttaggtgcg taccggctgg tccttaagtt ttcacccggt
    15061 tttaaccact acaatgttga tagcaaagcg ctaatgagct tccgttctct tcgggtttac
    15121 cgtgacaata aatccgtcac ttttgccgaa cttgatgtgt ttgatttgag tgaggcatta
    15181 agcgcacctg acgtcatttt ccattaaaac acagcgggca gtgatgcaac tgcccgttat
    15241 caacacgacc taccagcggg ggaagcgatg atcgtgcaaa aagagctggt tgctatttac
    15301 gattatgaga tccctgtacc tgaagagccg ttttccttca gacttgaaat ccataaatgc
    15361 tctgaattat ttacaggttc cgtctatcga ctggagcgat tccggctaca tccaacattt
    15421 catcaacgtg atcgagaaga tgccgaccac taataaatga tgcgttgatt tatataagag
    15481 atgagtttat tgatgagcgg aaattacgag gtgaatcaca tgaaacagta atagcaattt
    15541 ttaattgtga actacagaat atattcaacc aagaaataga ataatatact ctagataatt
    15601 caagtcgcag gaaggcgata agcgagtgag tccccaggag cttactaaag taagtgacag
    15661 gggtgaacga acgcagtcgc agcacatgca acttgaagta tggcgagtat agctttatta
    15721 aagggggaaa cggtattccc ccttagatgc gtttcaattt gcccgttact tcaacaaatc
    15781 aatcaaccgt aacagagcca ctaatagctc cagtacagca atgagcacgg ttaacttttg
    15841 tgtcagcgtg tccatccgct ctccttttaa ggagcgcaac accagcacct tacccttaca
    15901 ctgcctgtcg atactggttt acgtatgttg gaccacgatg ttgcactccg gatcaggcac
    15961 cacctgcagc accacccgtc cggtagccag gacttgaaat tggttcgatc cggcgcaacc
    16021 tcccaacagt caggattata aatctcatta tcttaattgc aacggggtcc agcggtggtt
    16081 ataattcgtt cgttggaaca cgatgttgca caggctgcgg tgttaggcct gaaagtagta
    16141 aataaaagaa cctgcctcac cagcaggttt ttttattttt tgcgatctgc tttccagata
    16201 ttttccgctc aaacaactaa tgcgccaaac atttattgcg cgtaaaatat cgtttatttc
    16261 attaatacat ttcagggatg aatatatgtc accgcagaat aatcacctgc agcgtccgcc
    16321 tgctgctgtg ttatacgccg ttgaactggc aaaattaaaa caaaatgata acgcaccttg
    16381 cccgcccggt tggcagttaa gtttgcctgc ggcccgtgct tttattcttg gcgacgaagc
    16441 gcaaaatatt cgccgtaaag tggtgattag tccctctgct gtcgaacgca tgttagtcac
    16501 tcttgccacc gggcgcggtt tgatgttggt gggggaaccg ggtaccgcaa aatctctcct
    16561 gtctgaatta ctggcaaccg ctattagcgg cgatgccggg ttaaccatcc agggcggcgc
    16621 atctaccacc gaagatcaaa tcaagtacgg ctggaactac gccctgctta tcaatcatgg
    16681 tccgtcaacg gaagcgctgg tccctgcccc gctttacctg ggaatgcgcg acggcaaaat
    16741 cgtccgtttt gaagagataa cgcgtactcc actggaagta caagactgtt tattaggtat
    16801 gctttctgac agggttatga cggtgccgga actcactggt gaagccagtc aactctatgc
    16861 tcgggaaggc tttaatatta ttgccactgc caatacgcgc gatcgcggcg tcaatgaaat
    16921 gagtgccgcg ctaaagcgtc gcttcgattt tgaaactgtc tttccgatta tggatttcgc
    16981 ccaggagctg gagcttgtcg ccagtgcttc ggcgcgtttg ctggcccata gcggcattcc
    17041 gcataaagtg cctgacgcgg tactggagtt actggtccgc acctttcgcg atctgcgtgc
    17101 caacggcgaa aagaaaacgt caatggatac actgacagcg attatgtcca ccgctgaagc
    17161 cgtgaacgtg gcccacgcgg tgggcgtccg cgcctggttt ttagcgaatc gagcaggtga
    17221 gccagccgat ctggtggact gtattgccgg aaccatcgtc aaagataacg aagaagaccg
    17281 cgcgcgcctg cgccgttact tcgaacaacg tgttgccaca cataaagaag ctcactggca
    17341 ggcttattat caagctcgcc accgcctgcc gtgaggaaag atgcatgagc gagccgttaa
    17401 ttgtcggcat ccggcatcat agcccggcct gcgcccggct ggtgaaatcg ttaatcgaaa
    17461 gccagcggcc acgatacgta ttgattgaag gcccggctga ttttaatgac cgggtagacg
    17521 aactgttttt agcccaccag cttccggtag ctatttacag ttattgccag tatcaggacg
    17581 gtgcggcccc cgggcgtggt gcctggacgc catttgctga attttcgccg gagtggcagg
    17641 cgctacaagc cgcacgtcgt attcaggcac aaacttactt catcgatttg ccttgctggg
    17701 cgcagagtga agaagaggac gattcgcctg atacacaaga tgaaagccag accttactgc
    17761 tgcgtgccac ccgcatggat aacagcgata ccctgtggga tcacttattc gaagatgaaa
    17821 gccagcaaac tgcattaccc tctacgctgg cgcactattt tgcccaactg cggggcgatt
    17881 cccccggaga tgctctcaat cgtcagcgcg aagcctttat ggctcgctgg attacatggg
    17941 cgatgcagca aaacaatggc gacgtgttag tcgtctgcgg cggctggcac gctcctgcac
    18001 tggcaaaaat gtggcgcaaa tgcccgcagg aaatgaacaa gccagaattg tcctcgctgg
    18061 cagatggcgt tacaggttgt tatctcacac cctacagtga aaagcgcctt gatgtgctgg
    18121 caggatacct ttccggaatg cctgccccgg tctggcaaaa ctggtgctgg cagtgtggct
    18181 tacagcaggc aggtgaacaa ctgctgaaaa cggttcttac ccgtttgcgc cagcacaaat
    18241 tgcccgcttc taccgctgat atggctgctg ctcatctgca tgcgatggca ctggcacagt
    18301 tgcgcggcca tacactacct ctacgcactg actggctgga tgccatagct ggctcgctga
    18361 ttaaagaagc cctgaatgcg ccgttgccgt ggagctatcg cggagttatt catcccgata
    18421 ccgatccgat tttgctaacg gtgatagaca cattagcggg tgacggattc ggtaaacttt
    18481 ccccttctac gccacaaccg cctctgccaa aagatgtcac ctgcgaactg gaacgtaccg
    18541 gaatctctct tccggcggag cttaccttaa atcgctttac ccccgatgga ctggcgcaaa
    18601 gtcaggtgtt acatcggttg gcaatactgg agatcccagg ggttgtgcgc cagcagggaa
    18661 gtacgctgac acttgcaggc aacggtgaag aatgctggaa attaacccgc ccgcttagcc
    18721 agcatgcggc attgattgag gccgcctgct ttggtgccac actccaggaa gccgcacgcc
    18781 ataaattaga agccgatatg ctggacgcgg gtggaatcgg ctgtatcacc acatgtctta
    18841 gccaggcagc gttagcgggt ctggcgtctt tcagtcagca attactggaa caactcacat
    18901 tattaattgc ccaggaaaat caattcgccg aaatgggcca ggcgctggaa gtgctatatg
    18961 ccttatggcg gctggatgaa attagcggta tgcaaggcgc gcagatatta caaacgacat
    19021 tatgcgcggc catcgatcgc acgctgtggc tatgtgaatc caacggcaga ccggatgaaa
    19081 aggagtttca cgctcacctg catagctggc aggcgctttg ccatatcctg cgcgatctac
    19141 atagcggcgt taatttaccc ggcgtttcgc tttctgcggc ggtagcctta ctggagcgcc
    19201 gcagtcaggc gattcatgcc ccggcgctgg atcgcggcgc gactcttggc gcactgatgc
    19261 gtctggaaca tcccaacgcc agtgccgaag cggcgctgac gatgctggcg cagttatccc
    19321 cggcacaatc cggcgaggcg ctgcacggtt tgctggcatt agcccgtcat caactggcct
    19381 gtcagccggt atttatcgcc ggtttcagca gtcatttaaa tcaactgagt gatgccgatt
    19441 ttatcaatgc cctgcccgat ttacgcgcgg cgatggcctg gctaccacca cgagaacgcg
    19501 ggacgctggc gcatcaggtg cttgagcatt accagctcac acagcttccc gtttcagcgc
    19561 tgcaaatgct gttgcattgt ccaccacaag ccattgcaca tcatcaacaa ctcgaacagc
    19621 aggcactggc atcgctgcaa cactggggag ttttccatgt ctgaactgaa cgatcttctg
    19681 accacccgtg agctacaacg ctggcgatta attcttggcg aagcggcaga aacgaccctt
    19741 tgtgggctgg atgacaacgc ccggcagata gaccacgcgc tggagtggct gtatgggcgc
    19801 gatcctgaac ggctccagcg tggtgaacgt tacggtggat taggtcgttc aaatctcacc
    19861 acccctgagt ggattaacag cattcacacg ctgtttccgc aacaggtgat tgagcggctg
    19921 gaaagcgatg ccgtgctgcg ctacggcatt gaagatgtgg tgacgaatct cgacgtgctg
    19981 gaacgtatgc agccttctga aagcctgcta cgcgccgttt tgcacaccaa acatctgatg
    20041 aatcccgaag tactggctgc cgcccgccgg atagtgcacc aggttgtgga agaaattatg
    20101 gctcgactgg caaaggaagt tcgtcaggct ttttctggag tacgcgatcg ccgtcgccgc
    20161 tcatttattt cactggcgcg aaactttgat ttcaaaagta ctctgcgcgc caacctgcaa
    20221 cactggcacc cgcaacacgg caagttgtat atcgaatccc cccgctttaa cagccgcata
    20281 aaacgctata gcgaacaatg gcaactggtc ctactggttg atcaaagcgg atcgatggtt
    20341 gattcggtga tccactccgc agtgatggcg gcctgtttat ggcagttacc cggcattcgt
    20401 acccatctgg tcgcttttga caccagcgtg gttgatctca cggcagacgt tgccgatccg
    20461 gtagagctat taatgaaagt gcaattaggc ggcgggacca atatcgccag tgctgtggaa
    20521 tatggtcggc aacttattga acaaccagcg aaaagcgtca ttatcctcgt gagcgatttt
    20581 tacgaagggg gtcatcatca ttgctgacgc atcaggtgaa aaagtgtgtc cagagcggca
    20641 tcaaagtgct gggactggcg gcgctcgata gcagcgcaac accttgctat gaccacgata
    20701 cggcccaggc gctggttaat gtcggcgcac aaatagccgc aatgacaccg ggcgagctgg
    20761 cttcctggct tgcggagaat cttcagtcat gaattcacta cgtccggaat tattagaact
    20821 gacgccgcag gctctgacgg cgttaagcaa tgccggtttt gttaagcgca gccttaagga
    20881 actggaaaat ggcaacgtcc cggaaatcag tcatgagaac ggcgctttaa tcgccacctt
    20941 cagtgacggt gtccgtaccc agctggcgaa cggccaggca ctgaaagagg ctcagtgcac
    21001 ctgcggggcc agcggcatgt gccgtcatcg cgtgatgctg gtgttaagtt atcaacgact
    21061 ttgtgccacc gcccagccta cggaaaaaaa agaagaagag tgggatccag caatctggct
    21121 gaaagaactg gctaacctgc ccgatgccac ccgcaaacgc gcgcaggcgc tggtcgctaa
    21181 aggcatcacc attgagttgt tctgtgcgcc tggcgaaatt ccctctgccc gattaccgat
    21241 gagcgatgtg cgtttttatt cccgcagcag cattcgcttc gcacgctgtg attgcattga
    21301 aggcacactt tgcgaacatg tcgtactggc tgtgcaggcc ttcgtcgagg ccaaaactca
    21361 gcaagcggaa tttactcatt taatctggca gatgcgcagt gaacacgtga catcatctga
    21421 tgatccgttt gccagcgaag aaggcaaaac gtgtcgtcaa tatgttcagc aattaagcca
    21481 ggcattatgg ctgggcggga taagtcaacc gccaatccat tacgaggccg ctttcagtcg
    21541 cgcgcagcaa gcggcggaac gctgcaactg gcgatgggtg agtgaatcgc tacggcaatt
    21601 gcgcgccagt gttgatgcct tccacgcccg tgccagccac taccatgccg gagaatgctt
    21661 acgtcagctt gcggcattaa acagtcgatt aaattgcgta caagagatgg cgcgacgcga
    21721 cagtatcggt gaagttcctc ccatgccgtg gcgcacggtc gttggcgctg gcattgccgg
    21781 agaagcaaaa cttgatcatc tgcggctggt gtctttaggt atgcgttgct ggcaggatat
    21841 tgagcagtat ggtttacgca tctggtttac cgatcccgac acaggaagta ttttgcatct
    21901 ttcgcgtagt tggcagagaa gcgaacagga aaactcaccg gcagcgacgc gtcggctgtt
    21961 tagttttcag gctggcgcac tggcaggtgg gcaaattgtt tcacaagctg caaaacgcag
    22021 tgccgatggc gagctgctgt tagcgacccg taaccgcttg agtagtgttg ttccactgtc
    22081 gccagacgcc tggcaaatgt tgagcgcgcc gttgcgccag ccgggcattg tggctttgcg
    22141 ggaatattta cgccagcgtc cccccgcctg catacgacca ctcaaccagg tcgataactt
    22201 atttattctg ccggtagcgg agtgtatttc gctcggttgg gatagcagcc gccagacact
    22261 ggatgcgcag gtaatcagcg gcgaaggaga agataatgtg ctgacgttat cccttccggc
    22321 atctgcctgt tctccttttg ccgttgaacg catggcggcg cttttgcaac aaacagacga
    22381 ccccgtgagt ctggtttctg gctttgtcag ttttgtagag gggcaattga cactggaacc
    22441 acgggtgatg atgacaaaaa cccgcgcctg ggcgctggac gcagaaactg cgcctgtggc
    22501 accgctacct tctgccagcg ttctgcctgt gccgtctacc gctcatcagt tgctgatgcg
    22561 ctgccaggcg ttacttattc aactgctcca taacggctgg cgctatcagg aacagagtgc
    22621 tattggtgag gcagagttgc tggcgaatga cctcaccgcg gtgggttttt atcggctggc
    22681 acatgtgttg ggacaatttc gtaatacaga aagcgaggca cgggtagaag caatgaataa
    22741 cggtgttttg ctttgcgaac aattattccc catgcttcag caacaaggat gaaatagtgc
    22801 tttttattaa gagttctact cctgttccgg actgctcacg ccacagtatt aggcatatcc
    22861 tatatagccc ctggtgagag tcaccagttc cttgattaaa taaaatggag ttttacatga
    22921 aggctttcaa taagctgttt tcccttgttg ttgcatctgt tctggttttc tctcttgctg
    22981 gctgcggtga caaagaagaa tcaaagaaat tcagcgccaa tctgaacggc actgaaattg
    23041 ccattaccta tgtctacaaa ggtgacaagg tgcttaagca atcttctgaa accaaaattc
    23101 aatttgcctc cattggtgca accaccaaag aagatgccgc caggacactt gagccgttaa
    23161 gcgccaaata caaaaacatc gcgggtgttg aagaaaaatt aacctatacc gatacctacg
    23221 cgcaggaaaa cgtgactatt gatatggaaa aagtggattt taaagccctg cagggtattt
    23281 caggaatcaa tgtttctgct gaagatgcca aaaaaggtat cactatggcg caaatggaac
    23341 tggtgatgaa agccgctggt tttaaagaag tgaaataatc tgtcggcggc catgtttcgc
    23401 atggccgcca tacccgtctt agcttttctt cacatgctgg cgcgctgcca gtccacgcag
    23461 aaaataacgt aaaaattgat cgccgcattc gcggaagttt ttatgatccg gtgcacgcat
    23521 catcgccgta atttccggca tcgaaacgcg gaactgctgt tcggtgagga ttgccagaat
    23581 gtcatcggtt ttaagcgaaa acgcgatgcg taattttttc agcacgatgt tgttattaat
    23641 gcgacgttcc ggctccagtg ccggagcaga ctcatccttg ccgcgttttt cataaatcag
    23701 gccattgagg aatgaagaca aaacaatgtc cggacaacgc tgaaaaccct cttcgtcttc
    23761 tttacgtagc cagacggcga tctgttccgc ggtggcttcg acattaccca gcgccagaat
    23821 acgcaccagg tcattattat tggctttcaa aatgtagcgc acgctgcgca gaatatcgtt
    23881 acttagcatg aggccttcag gtgttgatga ggcaaaaagc cattttagca gtcttttaca
    23941 ggccaatcgc ctcttttaag cttttcagat aacggcggct gaccggcacg gttaagccat
    24001 tacgcaaaat caactcggcc tggccgttat cttccagacg gatctcctgt aaatgcgcga
    24061 ggttaaccag atactgacga tggcagcgca gtaatggtgt acgactttcc agggtacgta
    24121 atgtcagttc ggtaaagccc tctttccctt cgtgactggt aacgtagaca ccgctcatcc
    24181 gactgctgac aaatgccaca tctttcattt gcagcaaata aatccgacta tgccccgtac
    24241 aagggataaa tttcagcgcc tgttgatttt ccggtaacag cgaaacatcc tgcttgctgc
    24301 gctcctgacg caatcgcgcc agcgttttct ccagtcgcgc ttcatcaatt ggcttcagca
    24361 gataatcaaa ggcatgttct tcaaaggctt taatggcgta ttcgtcaaac gcggtgagaa
    24421 aaacaatata cgggcgatgt tccgggtcaa gcatccccac catttccaga ccactgatgc
    24481 gcggcatctg gatatcgaga aacagcacat ccgggcgcag tttatgcacc gcgccgatcc
    24541 cttccacggc gtttgaacac tctccaacga tttcaatatc gctctgctcc tgcaaaaata
    24601 cgcgcaggtt ctcccgtgca agcggttcat catcgacaat taagacttta atcatgcctc
    24661 gtccctccat ggtagtcgta acgttattcg ggtgtaacta tcaggctcac aggcgacgct
    24721 tatcccatag tcatcgccaa accgttcacg taaacgctta tccaccagat tcatccccag
    24781 cccactggca ttggttaccg gttgatacaa accggcattg tcttcgatct ccagcatcaa
    24841 atgttgcccc tcacgtcggg cgctgattgc cactcgccct gtatccagca gttgtgatgt
    24901 cccatgttta atggcgtttt ccactatcgg ttgcagggta aacgcgggca attgctgctg
    24961 ggataattct tgcggaatag caatgttgac ctgcaaccgc gactggaagc gcgccttttc
    25021 aatttgcaga taagcgttca catgttcaat ttcgtcggcg agagtaacaa actccgaagg
    25081 ccgctttaag tttttgcgga aaaaagtgga aagatactgc accagctggc tggcctgttc
    25141 gctgtcgcgg cggatcaccg ctttaatggt gttaagcgca ttaaacaaaa aatggggatt
    25201 cacctgggcg tgaagcagtt tgatctctga ctgggtgagc atcgcttttt gccgctcata
    25261 ttgcccggcg aggatctgcg ccgaaagcag ttgcgcaatc ccctcgccca gcgtgcggtt
    25321 gattgaactg aataaacggt ttttggcttc atacaatttg atggtaccca tcactcgctg
    25381 attttcacca cgcaacggaa tgaccagcgt cgaccccagt ttgcattgcg gatgcaaaga
    25441 gcaacggtaa ggcacttcgt tgccatcagc gtagaccact tcaccggttt caatcgcttt
    25501 taaggtataa gtcgaagaaa tcggtttgcc gggtaaatgg tggtcgtcac caattccggt
    25561 aaaggccagc aatttctctc gatcggtaat cgcgactgca ccaatatcca gctcctgata
    25621 cagcacctgt gccactttca tgctgttcac ttcgttaaac ccctggcgca aaatgccttc
    25681 cgtcgaggcc gccactttca gcgcagtggc agaaaaagcc gaagtgtatt tttcaaacat
    25741 cgcgcgttta tcgagcaata tacgcataaa caacgccgcg ccgacggtat tggtgaccat
    25801 cattggcgca gcaatattac tcaccagacg caccgcatct tcataaggtc gggcgatcgc
    25861 caggatgatc aacatttgca ccatttcagc gacgaacgtg acggcaccgg cggtaatggg
    25921 gttaaagact ttatcagtgc gcccgcgacg gatcaggatg ctgtgtacca ggccgccgag
    25981 caatccttca acgatggtcg agatcatgca acttagcgcg gtcatgcccc ccatcgaata
    26041 tcgatgtaac ccgccggtca gaccaaccag cccaccgacg accggaccgc cgagtaagcc
    26101 gcccattacc gcgcctatcg cacgggtatt ggcaatagaa tcgtcaatgt gcaacccaaa
    26161 ccaggtgccc atgatgcaga agatggaaaa gacgatgtag cagagaaatt tatgcggcag
    26221 acgaaccgtg acctgcatta acggtatgaa taatggcgtt ttactcatta accacgcaat
    26281 gactaaaaaa acgcacatct gctgaagcag cagcaacacc agattaaaat cgtacatacc
    26341 cgcaaaccac acttcccttt aaaatgcgta acatacattg cttgcgttta actttctttg
    26401 aactcttgca gaaaaatgag aattcgtgag tacgatcact caaaatcgcc tggcaaaaat
    26461 aaaatcaccc tatagatgca caaaaaacgg gcaaaactac ctggttcgca aaactgcgtc
    26521 taaagttaaa ccgggacctc gcgagcaagg gtgagacgat ggcgctttac acaattggtg
    26581 aagtggcgtt gctttgtgat attaaccctg tcacgttacg cgcgtggcag aggcgttacg
    26641 gattgctgaa accgcaacgg acagacggcg gccatcggct gttcaacgat gccgatattg
    26701 accggatccg cgagatcaaa cgctggatcg acaacggcgt gcaggtcagc aaagtaaaaa
    26761 tgctgctcag taatgaaaat gttgatgtgc agaacggctg gcgcgatcag caagaaacat
    26821 tactgactta cctgcaaagc ggcaatctgc atagcctgcg aacgtggatc aaagagcgcg
    26881 gtcaggatta ccccgcccag acactcacca cacatctgtt tattcctctg cgccgacggc
    26941 ttcagtgcca acaaccgact ctccagacgc tgctggcgat cctcgacggc gtactgatca
    27001 actacatctc tatttgcctg gcttcggcgc gcaaaaaaca gggtaaagat gcactggtgg
    27061 ttggctggaa tattcaggat accactcgtc tgtggctgga gggctggatt gccagtcaac
    27121 aaggatggcg cattgatgtc ctcgcccact cgctcaatca actacgccct gaactgttcg
    27181 aaggccgtac attgctggtg tggtgcggtg ataatcgaac ctccgcccaa cagcagcaac
    27241 tcaccagttg gcaagaacaa ggccatgata ttttcccact cggcatttaa tgattcgtta
    27301 acaaatgcgc tttactgtac aatcctttcg ttaacataag gagtgcatta tgcgcatagc
    27361 taaaattggg gtcatcgccc tgttcctgtt tatggcgtta ggcggaattg gtggcgtcat
    27421 gctcgcaggt tataccttta ttttgcgtgc tggctgaacg cctgcaccag cctttcaaac
    27481 aggcggtctg cgatgatcgc cgccagtgcc accagtaacg ctccctggat cacatacgcg
    27541 gtattaaatc cgctaagtcc gatgatgatg ggcgtaccca gcgtgctggc ccctaccgtt
    27601 gaggcgatcg tcgccgtacc aatgttgata atcaccgaag ttcgcacgcc agccagaatc
    27661 accggagccg ccagcggtag ctcgacctta cgcagtcgct gaccacgact cattcccata
    27721 cctttcgcaa cttctgtcac gctggcatca atcgctccca gcccggcaag tgtcgcctgc
    27781 aggacgggca gcacgccgta aaggatcaag gcgataatcg ctggtttcag accaaagccg
    27841 atcaccggaa cggcaatcgc cagcaccgcg acgggcggaa aagtctgccc aacggcggca
    27901 atagtttcca ccagtgggcg aaattccgca ccccacgggc gagtgacagc aattccggca
    27961 ccagtaccaa tgatcaccgc aaacaaactc gaaattccca ccagccagaa atgagccagt
    28021 gccagagctg caaaactttc ttgctgataa acgggtcgtg gcagttgtgg gaacaaggca
    28081 gcaaacagcg gctggctata aggtagccag aaaatcagtg ccacaaacaa agcaatgagc
    28141 cagaacagcg gatcgcgcaa catcttcata cgcttacgcc tcctccagca gatcctgaaa
    28201 atgcagcgtg ccgcaaggct ggccctgcgt gttcaccacc ggcagcacct cgcatccccg
    28261 cgcgacaaac agagaaagcg catcgcgtag cgtcatcctt tcttccagtg cctcaccttc
    28321 agcccgttct tcacggcgca cgtaatctgc cacgctacgt aacgaaagca ggcgtacacc
    28381 cagctcacta cgaccaaaaa actggcggac aaaatcattc gccggatgag tcagcatcgc
    28441 cagaggattg ccctgctgca cgacttcacc gtgatccatc aataccagat gttctgccag
    28501 ccgtagcgcc tcatcaatat catgagtgac cagcacaatg gtacgcccct gcaaacggtg
    28561 aatgcgcgtc atctcttgtt gcaacgcgcc gcgcgttacc gggtccagcg cgccaaaagg
    28621 ttcatccatc agtaagactt gcggatcggc agccagtgcg cgcgccactc ccacacgttg
    28681 ctgctgacca ccggaaagct gatgcggata acgctcacgc aaatttggct ccagccccag
    28741 tagcgccatt aattcgtcga tacgatcgtc gatccgcgcc cgtgaccatt tttgcaattg
    28801 cggcacggtg gcgatgtttt gtgccacgct ccagtgggga aacaggccaa tagattgaat
    28861 ggcatagccc atccggcggc gcaactccag tactggcagc gagcgaattt cttctccggc
    28921 aaagcggatc accccgctgt catgctccac caggcggtta atcattttca gagtggtgga
    28981 tttaccggag cctgatgtac caatcagcac cgaaaaactc ccttcctgaa aattgagatt
    29041 gagatcgtta acggcttttt gtgcgccgaa cagtttgctg acatggctaa attcaatcat
    29101 tacgtttcac cttcagcagt gcgataagca aatcgaacag cgcgtcggta agcaccgcca
    29161 gaacaattac cgggatcacc cccagcaaca ctaaatcaat ggcactgctt agcagcccct
    29221 ggaaaaccag cgcaccaaaa ccgcctgcgc cgattaacgc cgcaatcacc gccatgccaa
    29281 cggtttgcac catcaccacc cgcaggctgc gcagaaatac cggtaacgcc agcggtaact
    29341 gaacatgcag gaaacgctgc accccgctca tccccatcgc tctggcgctc tccagcacat
    29401 cgcgcgggat ctggttcaag ccgactacta cgccgcgcac cagcggcagc aaggcataga
    29461 gcaccagcgc aataagtgcg ggtgtcattc cggttcctgc tatgccgagc gttcccagcc
    29521 acggaaaggc cgtaaccagc gcggcaagcg gcgcaatcag caggccaaag agcgctaccg
    29581 aaggcacggt ctgaatgaca ttcagcagag aaaaaatcgc cccctgccgc gcggtggaaa
    29641 agtagcacca gatgcccaac ggcacaccaa tcactaacgc aggcagcacc gcaccaaaca
    29701 gcaacgtcag atgttgtgcc agagcgtcgt caaacacatc ctgacggttg gcgtattctt
    29761 tcattaacga gagatcgtta agcgtgccgg agtacagcaa ccacagcgga ataatggcaa
    29821 tctgcatatg caacaaccag cgccacagcg gatgcgtgga gattcggcgg atggcatcgc
    29881 tacaggccag cagtgccagc gccgcagcca gccagaaacc actgccgagg ctggtacgtg
    29941 ccagcgcact gccattttgc gccagttgtg tcgccgcctt tccagctccc cacaccagca
    30001 atacgaagac gaattgcgcc agaatgagtg caaaaatgct gcctttttta gcgggaataa
    30061 aacaggctgt cagccaggcg caaccaacgc ccaccagcat ccagagagtt tgcggccaca
    30121 gctgccagag atgacgcccc tcacccgaaa ctaaacgatt aggcgcgtaa ctgataaacg
    30181 gcagcgccgc tgcgattgcc gtcagcaaca gcagcagcgc cagaacagga ttaatacgta
    30241 gataagtcac gggaaattac ttcgtccacc ctttttgttt caggtagtcg gcagccactt
    30301 ttttggcatc cagtccttcc acagcaatgc tggcattcag ttgctgcaat gttttttcat
    30361 cgaggctggc gaagactggc tgtagccact gtgccatttg tggatactct ttcagcaccg
    30421 actcacgcac cactggtgca ggcgcgtaga taggttgcac gccttgcgga tcgcttaagg
    30481 tttgcagccc cagcgccgcg accgggccgt cagtgccgta agccattgcg gcattaacgc
    30541 cagaggtttg ctgggcagcg gctttgatcg ttaccgccgt gtcgccacca gccagtgaca
    30601 gcaactgatc ctgaccgagc ttaaagccat aggctttttc aaacgcgggt aaggcatcgg
    30661 cgcgttcaat aaactctgcc gaggctgcca gtttgaaggt gccgccctct tgcagataac
    30721 gactcaggtc ggcaagcgaa gtgagcttgt ttttctctgc cacatcctga cgcacggcga
    30781 tggtccaggt gttatttgca ggtgcgggcg tcagccagat taacttgttt tgctctgcat
    30841 cgagtttttt gactttctcg taaccttgct gcacgttttt ccacgctgca tcgttttcat
    30901 ctttaaagaa gaacgcccca ttgccggtat attccggata gatatccagc tcgccagaag
    30961 tgatcgcgcc gcgcaccaca ggagtcgttc caagttgcac tttatttacc gtaggaacgc
    31021 cgtgactttc cagcacctgc aaaatgatat tgccgagcag cgcgccttcc gtatcgattt
    31081 ttgaaccaac cttaacgggg gaagctgcct gtagcggcag gctcacggct gccaacaaaa
    31141 ccagtgaacc tgcccagacc tttgagagtg gcatgatgct ttcctcattc ttttactgtt
    31201 gttttcagcg aattaagaga aaagcatagt tgataatgac agggttagcc tgtctgggtg
    31261 gattcagttg cagaatcaga taaatactta aaaggcatat tttgcccggc gtttcttgcc
    31321 ggatgcggcg taaacgcctt atccggccta caaacatcga aaattacaac aactcaaact
    31381 cggctttctt aacgcgtgcg gaatcagtgc cgataaagac attgaacttg ccaggctcgg
    31441 cgtcatattt catctgttga ttccagaact tcagcgcctc aatatcaatc gggaagctga
    31501 cggtctgagt ttcgcccggt ttcagagtga ttttctcaaa gcctttcagc tgcttcaccg
    31561 ggcgactcat ggaagccgtc acatcctgca agtacatctg tactaccgtg gccccttcgc
    31621 gtttaccggt gttcgtcacc tgcacgctgg ccgtcacttt gccgtcacgc ttcatggtcg
    31681 gcgcagaaag tttcacatca gagacggtga aagtggtgta gctcagccca tagccgaacg
    31741 gataaagcgc cccgttagct tcatcaaaat aacgcgaagt gtatttgttc ggcttgtcgg
    31801 cattatacgg acgcccggta ttcagatggc tgtagtacac cgggatctgc ccgacagaac
    31861 gcgggaagga catcggcagc ttgccggacg ggttataatc gccaaacaat acatcggcaa
    31921 ttgcattacc gccttcagtc cccgcaaacc aggtttccag aatcgcatca gcctgctgat
    31981 cttctttcac cagcgccagc ggacgcccat tcatcagcac cagcaccagc ggtttaccgg
    32041 tggctttcag cgccgcaatc aagtcacgtt ggctttgcgg aatagtgata tcggtacgac
    32101 tggaagcttc gtgcgccatc ccctgagctt caccgaccac agccaccacc acatcagatt
    32161 gtttcgcggt ttgcaccgct tcatcaatca tctcttgcgg cgaacgcgga tcgactttga
    32221 ccgcttcttc atactgattc aggaaatcga taatgccttt gtcactggta acgttcgccc
    32281 ctttggcata cagcacttta ccgttttcac cgacggcgtt tttaatcccg gttagcacag
    32341 taacagattg atcggcgaca cctgctgccg accagcttcc catcacgtca cgcttgctgt
    32401 ctgccagcgg gccaaccacc gcaatggttg cagatttttt cagcggcagc gtttcgagac
    32461 ggtttttcag caacaccagg ctttcgcgcg ccacttcacg cgcttctttg cggtgcaggc
    32521 ggctttcggc attggtatcc accgggtcag actctttcgg ccccaaatgg ctgtatggat
    32581 cgttaaacaa tcccatatca tatttaacgt tcagtacatg gcgggcagca tcgtccagct
    32641 cttccatcgt cactttgccg gatttgatca acccaggcag atacttcgag taatactcgt
    32701 cgctcatgct catgttgatt ccggacttca gcgccacgcg taccgcatcc tctgggtctg
    32761 ccgccgtgcc atgtttaatc agttctttga ttgcgccgtg atcggaaacg gtgatgcctt
    32821 taaagcccca ctggtcgcgc agaacatctt tcagcagcca ggagtcggag gtggctggcg
    32881 tgccgttcag cgagttcagc gccaccatca ccgcaccgct gcctgcatcc agccccgctt
    32941 tgtacggcgg catatagtcg ttaaacaggc gctgcggact catatcgacg gtgttgtact
    33001 ctttaccgcc ttctaccgcg ccgtatgcgg caaagtgttt gacgctggtc atcaccgagt
    33061 agcgatctgc cgggctttta ccctgcatcg cttccaccat ggttttaccc attattgagg
    33121 tgagatacgt atcttcgcca aaaccttcgg aagcacgtcc ccagcgcgga tcgcgcgaga
    33181 catcgaccat cggtgcccag gtcatattca ggccatcatc tgccgcttca taagcagaga
    33241 cacgcccgac tgttttcacc gcatcgaggt taaaagacga ggccagaccg agactaatcg
    33301 ggaacaccgt gcgctgaccg tgcagcacgt cgtaagcaaa gaaaagagga attttcaggc
    33361 ggcttaattc catcacctga tcctgcatgg cgcggatatc ctgacgggtt acggtgttga
    33421 aaatcgcccc aacctgaccg tctttgatca tctcgcggat cgcctctttc gggttatccg
    33481 ggccgacgct gattaagcgc agctgaccaa ttttctcatc aactgtcatt ttcttaagca
    33541 gttcggtgac gaacgcatcc cgcgcttcgg gcgttaatgg atggttgccg aataaatcat
    33601 ccgccagtgc tggctgcagg gccagactca ccgcgattcc tactgaacat agccatttca
    33661 tatggatttt tcttcctttg ttgcccgacg tggcagcgaa aatggtgcaa aaaccgtagt
    33721 ttgccataag catgatggag agagaaaaag aatgctcagt ttattgtctg aattttcaaa
    33781 atattcactc gctgaattgt catacaaggc gctattctag tttgtgatat tttttcgcca
    33841 ccacaaggag tggaaaatgt cttccatgac aacaactgat aataaagcct ttttgaatga
    33901 actttcccgt ctggtcggtc attcacacct gctcaccgat cccgcaaaaa cggcccgcta
    33961 tcgcaagggc ttccgttctg gtcagggcga cgcgctggct gtcgttttcc ctggctcact
    34021 actggaattg tggcgggtgc tgaaagcctg cgtcaccgct gacaaaatta ttctgatgca
    34081 ggcggccaat acaggcctga ccgaaggatc gacgccaaac ggtaacgatt atgatcgcga
    34141 tatcgtgatc atcagcaccc tgcgtctcga caagctgcac gtactcggta agggcgaaca
    34201 ggtgctggcc tatccgggca ccacgctcta ttcactggaa aaagccctca aaccgctggg
    34261 acgcgaaccg cactcagtga ttggatcatc gtgtataggc gcatcggtca tcggcggtat
    34321 ttgtaacaac tccggcggct cgctggtgca acgtggcccg gcgtataccg aaatgtcgtt
    34381 attcgcgcgt ataaatgaag atggcaaact gacgctggtg aaccatctgg ggattgatct
    34441 gggcgaaacg ccggagcaga tccttagcaa gctggatgac gatcgcatca aagatgacga
    34501 tgtgcgccac gatggtcgtc acgcccacga ttatgactat gtccaccgcg ttcgtgatat
    34561 tgaagccgac acgcccgcac gttataacgc tgatccggat cggttatttg aatcttctgg
    34621 ttgcgcaggg aagctggccg tctttgcagt acgtcttgat accttcgaag cggaaaaaaa
    34681 tcagcaggtg ttttatatcg gcaccaacca gccggaagtg ctgaccgaaa tccgccgtca
    34741 tattctggct aatttcgaaa atctgccggt tgccggggaa tatatgcacc gggatatcta
    34801 cgatattgcg gaaaaatacg gcaaagacac cttcctgatg attgataagt taggcaccga
    34861 caagatgccg ttcttcttta atctcaaggg acgcaccgat gcgatgctgg agaaagtgaa
    34921 attcttccgt ccgcatttta ccgaccgtgc aatgcaaaaa ttcggtcacc tgttccccag
    34981 ccatttaccg ccgcgcatga aaaactggcg cgataaatac gagcatcatc tgctgttaaa
    35041 aatggcgggc gatggcgtcg gtgaagccaa atcgtggctg gtggattatt tcaaacaggc
    35101 cgagggcgat ttctttgtct gtacgccgga ggaaggcagc aaagcgtttt tacaccgttt
    35161 cgccgctgcg ggcgcagcaa ttcgttatca ggctgtgcat tccgatgaag tcgaagacat
    35221 tctggcgctg gatatcgctc tgcggcgtaa cgacaccgaa tggtatgagc atttaccgcc
    35281 ggagatcgac agccagctgg tgcacaagct ctattacggc cattttatgt gctatgtctt
    35341 ccatcaggat tacatcgtga aaaaaggcgt ggatgtgcat gcgttgaaag aacagatgct
    35401 ggaactgctt cagcagcgtg gcgcgcaata ccctgccgaa cataacgtcg gtcatttgta
    35461 taaagcaccg gagacgttgc agaagtttta tcgcgagaac gatccgacca acagtatgaa
    35521 tccggggatc ggtaaaacca gtaagcggaa aaactggcag gaagtggagt aataattacg
    35581 gatggcagag tatcgccatc cgtcattcat ttaatcgttc tgtgccgtct gccccgccgc
    35641 cgccatttgg gcggcttttt gttttttata gctcaacgct gctgccggaa caggcatcac
    35701 tttaccggtt tcaatccagg tacgcaggcg gctggcatcg gcaaaatggg tatatttgcc
    35761 aaacgcgtcc atcactacca gcgccaccgg tttattattg ataaccgtgc gcatcaccag
    35821 acaatggccc gccgcattgg taaaaccggt tttggttaac tgaatattcc agttatcacg
    35881 atacaccaga tggttagtat tgcggaacgg cagcgtatac gtcgggttag agaaggttgc
    35941 catatcttcc cgggtagtac ttaactgccc gatcaacgga tattgtttgc tggcaatgag
    36001 tagtttggtt aagtcacggg cggttgaaac gttatgcacc gacaatccgg ttggttcaac
    36061 aaagcgcgtg ttgttcattc cgagcgattt cgctttcgca ttcatcgcct taataaaggc
    36121 tttgtaacca ccgggataat ggtgcgcaag gcttgccgcc gcgcggtttt ctgaagacat
    36181 cagcgccagc aacagcatat ctttacggct gatttcgctg ttcagtcgta cgcgcgaata
    36241 gacccctttc atctccggcg tctggctgat atccactttt agtttttcat ccagcggcag
    36301 tcgtgcatcc agcacaacca tcgcggtcat taatttgctg atagacgcaa tcggacgcac
    36361 cagatccggg tggttcgaat agatcacttt gttggtattc agatcaacaa tcatcgcgct
    36421 accggaggca atttccggtt gtgacgcggt ggtagcggct gccgttttcg caacggcctg
    36481 cggtgcaaaa ggcacagcca gcatcagggc caggctaaat aaagaaactc gaaatttcgg
    36541 catgatgagc attcagatag tggttcacgc gcacgggttg cgcaccgccg gagtaaggat
    36601 ttactgaggc tagcgacgcc atcataacga gcaaaaagtg cgatcgtcaa aggagaatcg
    36661 tgaggaaatg ctgcattgct gacatttacg ccagcaatgc agcgcaaaag aactttctag
    36721 aacaaacgat aaccgtagcc ccatagtata acggtaaggg cgagcagcac ttccagcacc
    36781 agcacgccta tcgccagtgt cgaactggag aagctaagtc cttcctcttt gttgatattc
    36841 aggaagctcg gaataccgag atacagcagg tagccggtgt aaaacagcgc caccgtgccg
    36901 accagcgcac acaaccagac cagcgggtaa agcgccacca gaccgcttaa aaacagcgga
    36961 gttgcaacgt agcccgcgaa gaccatgcag tgcgcaagtg acggacgctg cggataatta
    37021 cgcgccatcc accagatgac ccgccccatc accgcaaccc cagccagcat aacgccgtaa
    37081 aacaagacag ccagcgccag tccggtaaac caggataact tcaggatagt gccatcgcca
    37141 aaattccagc caatctgtgt agtgccaatg aaggcgcaaa tcaccggaat cgccgccatc
    37201 agcaaaacgt ggtgggtgta gtgatgagaa atcgtttcgt tttcgcgatt aatcacctgc
    37261 atttcacgat cgggatggga aaacagtccc cagacatggc tcataccgcc cccttgttgt
    37321 gagttcatga acctgacagt tcaagtataa gtcagcttgt gattattttt tgttcactca
    37381 tgtaatttgc accgtctgat gtacgtcctt tttcagcgtg aatgattcac agggtgtatg
    37441 cttacaggca agcaaaggga gacagactgt cctatggatc tcaatacact catctcacaa
    37501 tatggttatg ccgcgctggt gatcggtagc ctggcggaag gtgaaaccgt gactttgctg
    37561 ggaggcgttg cggcacatca ggggctatta aagttcccgc tggtggtact ttctgtggcg
    37621 cttggcggca tgattggcga ccaggtgctt tatctgtgcg ggcggcggtt tggcggcaag
    37681 ctattacgcc gtttctcgaa acatcaggat aaaattgagc gggcgcagaa actgatccaa
    37741 cgccatccgt atctgtttgt cattggtacg cgttttatgt atggctttcg ggtcattggc
    37801 ccgacgctga ttggtgccag ccagctgccg ccgaaaatct ttctgccact gaatatcctc
    37861 ggcgcatttg cctgggcgtt gatttttacc actatcggtt acgctggtgg tcaggtgatt
    37921 gcgccgtggt tgcataatct cgaccagcat ttgaagcact gggtctggtt gattctggtc
    37981 gtggttctgg tggtaggcgt gcgctggtgg ctgaaacgac gcgggaagaa aaagccggat
    38041 aatcaggcgt aaaaccattg cctaggataa ggcgttcgcg ccgcatccga catccggtgc
    38101 accatgcctg atgcgacgct tgtgcgtctt atcatgccta cccaaatatt acccattgaa
    38161 ataaccacgc acctcactgc gctattctgg gttgaactgc ggattcgcca gcataaagcc
    38221 gccatccact atcaacgact gcccagtggt gtaatttgcg ccctccgaac aaagccacac
    38281 caccaggctg gcaatctcat gcgttgcgcc aaaacgccgc aagggaatcg aaggctccgc
    38341 gccgggcttc acgtcgccat catccatgcc gttcattggc gtggcgatcg ccccaggtgc
    38401 aaccgcattc accaaaatct tatgcctgac cagctccagc gccatcgctt tggttaaccc
    38461 gccgagcgca tgtttagcgg ctgtgtaggc gctggcatcc ggcaacggcg tatgttcatg
    38521 caccgacgta atgttgatga tgcgaccacc ctgcccttgt ttcaccatct ggcgggcagc
    38581 aatttgcgag cataaaaatg caccatcgac atcaacggta aaaatcttgc gccactcatc
    38641 gaaagccata tcaagaaacg gcgctttggt cattgcgccc gcattattca ccagcacatc
    38701 aatacgcccc agccgttgaa tgagtttctc cagcgccagt gccccttctg gtagattgcc
    38761 gagatccagc tgcacgatct ccgcacgtac gccgtggcta actacctcac gcgcggtatc
    38821 ttttgcccct tcttcatctg agtgccaggt aatgccaata tcaaacccct gctgcgccag
    38881 taataacgcg cactctttgc cgatccccga atcggaggcg gtaataatcg cgacctgtgc
    38941 catcgagttc tccacttaac gctgaataaa cgttaagtat agaaggcgca tatcatcagc
    39001 gtttgtaacc cccgcccaac gcaccagtga gttgaatgga ggcatccagc cactgccctt
    39061 gcaataacag gccattggcc cgctcacgca gcgcggggat tctggcttcg ctgacgcggg
    39121 aacccgcaat gatgcccgcg ttaaagcgcg cctgcgcaag acctactaca cgcaaggcat
    39181 cgcgctcaat ttgcgcctga tgctggtttt tctccgccag tgtctgaacc tgactggctg
    39241 cccgcgccac gtcattcacc gcttcaacca ccgctttgtt gtagctggcg atagacaagt
    39301 tgctttcggc ttttgcgata tcgaggttgg cgttaagacg accactatcg aaaatgggta
    39361 gcgtcaggcc tgccgtaacg cccatttgct gcgcggaatg acggaacaga tcgcttaagt
    39421 gcaacgcatc ctgttgcagg aaggccatca ggttgatgtc aggataaaat gccgcttttg
    39481 ccgcatcaat agtgcttaac gatgactcaa cgtaccagtg cgccgcctgc aaatctgccc
    39541 gccgggccag taaggagtac cccagttcat caggaagctg acttgccact ttcggcaacg
    39601 cgaccggatt aagcttcaat gcctttgtct ggtgatttgt aagtgcgctt aaccgtgcct
    39661 caataatttt cattttcccc gcgacatcgt tgagctgctg ccgggttttg ctggcattaa
    39721 tatcggtttc cacaccttca actgaagaag taatcccgtt ctgatatagc tggcgatcgg
    39781 tggcgataat ggtgttctgc tctttttcga tttgctgtaa gacagtgttt aacgccgcct
    39841 gggtttgcca ctcccagtac aggcgggcta cgctgccagc cagcaattgg cgggtttgct
    39901 cgcgttccgc cgctcgtgct ttaaccgtac ccaggcgagc agttacctct gcccgattct
    39961 ttccccagat atcgagatgc cagcccgccg ttaagccaaa agtaccgttg gtgtaccacg
    40021 gaccggtcgt acctgcggca ggatcgttca gagcaaacgg ccccattaac ccttctgcag
    40081 acattttttg ccgctccata tccgcagaaa agtcgatctg cggaccatcc tgagtagcaa
    40141 ctgccttcgc ctgggcttca gccagttgaa tgcgctgttc agccacctgc atatccggtg
    40201 cgttctgtag tgcattgtta attaaggaag tgagttgatt atcgtgatac tccagccacc
    40261 attggctgtc tggccaacca tttttcagcg ccgtgggtaa tgcggtgtca acttgtgcag
    40321 cgggcgtttg ctggcttaac gcctggcggg tttcatgcat aggtgcacac ccggccagca
    40381 tcagtaacag cggaaaacag gcgatggcgg gataaaagga atcacgattc atgggggaat
    40441 aatcaggtaa gaaaaggtgc gcggagatta ccgtgtgttg cgatatattt tttagtttcg
    40501 cgtggcaata catcagtagc aataaaacga catatccaga aaaatataca ctaagtgaat
    40561 gatatcttcc gatttatctt aatcgtttat ggatagcggc aaagggcttc gttttttcct
    40621 atacttattc agcactcaca aataaaggaa cgccaatgaa aattatactc tgggctgtat
    40681 tgattatttt cctgattggg ctactggtgg tgactggcgt atttaagatg atattttaaa
    40741 aatcagatat ggcatataag ttgtcaggtc aggaactaga tattttgtgt acttgatttg
    40801 caaacagctc cggtgttggt ggcgtcaaaa tcgctgaagc gttccccgaa ggccggggca
    40861 gcccacatgg atgtgggctg agggcgcgtc ttacaaggac gttacctcgc gcccgacccg
    40921 gtagccgaag gggataagct gaaggtaccg cgcagcggcg attttgttcg ccagagcccg
    40981 ggggtgcagg gggaggaggc gattgtcctc cccctgcgcg ctccttgcgc cagtggcaat
    41041 atgtggctta gctcatgaaa ggagcgcaac aagatgatga agctgaataa gcgattcatt
    41101 gtctcagata agctgcgatt ccactattat cgcagcttat cgatatcaat ggcctgaata
    41161 gcccttgcaa tatcagggga attattcaac acccgaacat gctgaaataa ttccgttgct
    41221 tcatcgtatt ctttacgcaa ataactcaac cactgtttaa tccgcgcaac gtgatataac
    41281 ccggtatcgc cctgcttttc cagacgggta tatttttgca gcaaagcaac cacctccggc
    41341 cacggcattc gcggttcgtt atatttcacc acacggctca ggttgggaat attgagcgcc
    41401 ccgcgaccaa tcatcactgc gtcgcagccg ctgatcgcca tgcattgttg cgcactctgc
    41461 cagtcccaga tttcaccgtt ggcaatcacc ggaatattaa gccgctggcg aatctcgcca
    41521 atcgcctgcc agtcaatatg ttccgcgcgg taaccctgct cttttgtccg cccatgtact
    41581 accagctccg tagcaccagc ctgttgaacc gcatcggcga tttcaaattt cttctcaccg
    41641 ctgtcccagt ccagacgcac ttttacgctg acaggcaaat gcgccggtac agcttcgcgc
    41701 atcgcttttg caccctggta gatgagttca ggatctttca gtaacgttgc cccaccaccg
    41761 ctaccgttaa ccgttttcga cgggcagccg cagttaagat ccacgcccca ggaacctaac
    41821 tccaccgcac gggcggcgtt ctctgccagc cactgtggga actgccctaa caactgcacg
    41881 cgaaccagcg taccggatgg tgtccggctg gcattttgta gctcagggca aatgcgatga
    41941 aagactttta ctggcagcag ttgatccacc acgcggacaa actcggtgat gcacagatcg
    42001 tagtcgttaa cttcggtcag taattcccgc accagagagt caagtacacc ctccatcgga
    42061 gccagtaaca cacgcatatc atcacccgca aaaaaatgag gcgctatgtt agcgcctccg
    42121 gtcaacggat taaaggtctg caaagaggga ttgctgcaaa ctgcaatccc tctctatgct
    42181 tgatgtatgc ctgagttcgc tgcgcattgc gtaataccgg ggagcgaaaa aataagcgct
    42241 gatttcttaa tgtgatcggt agcacgtttt acgaattaat tgtatgatga atccatctca
    42301 tctggggtgt tgattatgag taagacactg aacattatct ggcaatattt acgtgctttt
    42361 gtcctgattt atgcctgcct gtatgcaggc attttcattg cttccctgct accggtaacc
    42421 attccgggca gcatcatcgg gatgctgatc ctgtttgtcc tgctggcctt gcaaattctt
    42481 ccggcgaaat gggtcaatcc ggggtgctac gtactgattc gctatatggc gctattgttt
    42541 gtgccgattg gtgtaggcgt catgcaatat tttgatttgc tccgcgcaca gtttggtccg
    42601 gtagtggttt cctgtgcagt cagtacgctg gtggtttttc tggtggtgag ctggagttca
    42661 caactggtgc atggtgaacg taaagtcgta ggtcagaaag gatcagaaga atgatggcga
    42721 atatctggtg gtcattaccg ctgacgttga ttgtcttttt tgccgcccgc aaactggcgg
    42781 cacggtataa gtttcccctg cttaacccgt tgctggtagc aatggtggtg atcattcctt
    42841 ttttaatgct gaccggcatc tcttacgaca gctactttaa aggtagcgaa gtgctaaacg
    42901 atctgctgca accggcggtc gtcgcactgg cctatccttt atatgagcag ctacaccaga
    42961 tccgcgcgcg ctggaaatcg atcatcacca tctgttttat cggcagcgtg gttgcgatgg
    43021 tgacggggac ttctgtggca ttgttgatgg gcgcttcacc ggaaatcgcc gcgtcaatcc
    43081 tgccgaaatc agtcaccacg cctattgcaa tggcagttgg cggcagtatt ggcggtattc
    43141 cggcaatcag cgcagtttgc gtgattttcg tcggcatcct cggcgctgtg tttggtcata
    43201 cattgcttaa tgcgatgcgt attcgtacca aagctgcgcg cggtctggca atggggacag
    43261 cctcgcacgc cctcggtacg gcgcgctgcg ccgagctgga ttatcaggaa ggtgcattta
    43321 gttcgctggc gctggtgtta tgcgggatta ttacttcgct gatcgcaccg ttccttttcc
    43381 cgattattct ggcagtaatg ggctaaaatt tgcgatgcgt cgcgcatttt tgatgtaagt
    43441 ttcacgcgtt gcataattaa tgagattcag atcacatata aagccatagc gggttcgtaa
    43501 actgttatcc cattacatga ttatgaggca acgccatgca tccacgtttt caaaccgctt
    43561 ttgcccaact tgcggataac ttgcaatctg cactggaacc tattctggca gacaagtact
    43621 tccccgcttt gttgaccggg gagcaagtct catcgctgaa gagcgcaacg gggctggacg
    43681 aagacgcgct ggcattcgca ctacttccgc tggcggcggc ctgtgcgcgt acgccattgt
    43741 cgaattttaa tgttggcgca attgcgcgcg gtgtgagcgg aacctggtat ttcggtgcca
    43801 atatggaatt tattggtgcg acaatgcagc aaaccgttca tgccgaacaa agcgcgatca
    43861 gccacgcctg gctgagtggt gaaaaagcgc ttgcagccat caccgttaac tacacgcctt
    43921 gtggtcactg ccgtcagttt atgaatgaac tgaacagcgg tctggatctg cgtattcatc
    43981 ttccgggacg cgaggcacac gcgctgcgtg actatctacc ggatgccttt gggccgaaag
    44041 atctggagat taaaacgctg ctgatggacg agcaggatca cggctatgcg ctgactggtg
    44101 atgcgctttc tcaggcggcg attgcggcgg caaaccgttc gcacatgcct tacagtaagt
    44161 cgccaagcgg tgtcgcgctg gaatgtaaag acggtcgtat tttcagcggc agctacgctg
    44221 aaaacgccgc attcaacccg actctgccgc cgttgcaggg ggcgttaatt ctgttgaatc
    44281 tcaagggtta tgattacccg gatatccagc gcgcggttct ggcagaaaaa gccgatgcgc
    44341 cgttgattca gtgggatgcc acctccgcaa cgctgaaagc tctcggctgt cacaatatcg
    44401 accgcgtgct tctcgcttaa gcctggtgcc ggatgcggcg tgaacgcctt gtccggcttg
    44461 ccagcccttt cctggtgtcg aaaatcccgg caaacagttt gccgtttctt gcgcaaaacc
    44521 agcgggtaaa gtagcctgat ggaaattttc cttagatcga gtctcctgca tgttaaagcg
    44581 cgtgttcctc agcctgttag tcctgatcgg cttgctgctg ttgactgtgc tcggcctcga
    44641 tcgctggatg agctggaaaa ccgcgcctta tatctacgac gaattgcagg atctccccta
    44701 ccgccaggtc ggtgtggtgc tcggcacagc aaaatattat cggactggcg taattaatca
    44761 gtattatcgc taccgcattc aaggagcgat taatgcctat aacagcggta aggtaaatta
    44821 tctattactg agcggcgata acgcattgca aagttataat gagccgatga ccatgcgcaa
    44881 agatttaatc gcagccggtg tcgacccatc agatattgtt ctcgattacg caggctttcg
    44941 tacgctggat tccatcgtac gtacacgcaa agttttcgat actaatgatt tcattattat
    45001 cacccaacgt ttccactgtg agcgggcatt atttattgcg ctgcatatgg ggattcaggc
    45061 tcagtgttat gccgtaccgt caccgaaaga tatgctgtca gtacgtattc gcgaatttgc
    45121 cgcccgtttc ggtgcgctgg ctgaccttta tatttttaaa cgtgaaccgc gttttttagg
    45181 gccgctggtc cctattccgg caatgcatca agtgccggaa gatgcgcagg ggtatcccgc
    45241 tgtcacaccc gaacagttac ttgaattaca aaagaaacaa ggaaagtagt tatggatgta
    45301 cagcagtttt ttgtcgttgc cgtttttttc cttatcccga tattttgttt ccgcgaagca
    45361 tggaaaggct ggcgcgcagg cgcgattgat aaacgggtta aaaatgcacc ggaaccggtg
    45421 tatgtctggc gagcgaaaaa tcccggactc ttcttcgctt atatggtggc atatatcggc
    45481 ttcggaattt tatctatcgg catgattgtt tatcttattt tctatcgtta atattttctc
    45541 ttttctgtta attattgaga ataattatta cttcactcaa taagctgcgg atataacatt
    45601 ctgaaccgca gctatttgtg aatcctttca cagtttaaat ttccccacgc gcatagcctt
    45661 aatatcagca cgcaatattt atttagcgtt cgccttaatt actcatagca ttaaggaaga
    45721 tcacatgccg caacaaaatt atctggatga actcactccg gcttttacgc ctttactggc
    45781 gattaaagaa gcctctcgct gtttattatg tcatgacgct ccctgtagtc aggcttgccc
    45841 ggcacagacc gatccgggga aatttattcg ctcaatctac tttcgtaatt ttaaaggcgc
    45901 tgccgagaca attcgcgaaa ataatgccct cggtgccgtt tgtgccagag tgtgcccgac
    45961 ggagaaatta tgccaaagcg gttgtacacg tgccggtgtt gatgctccca ttgatatcgg
    46021 tcgcttacag cgttttgtta ctgattttga acaacaaact gggatggaaa tttatcagcc
    46081 cggtactaaa acgctcggca aagtcgccat tattggcgca ggtcctgccg gattgcaggc
    46141 cagtgtgacg ctgacaaacc agggttatga cgtcacgatc tatgagaaag aagcacaacc
    46201 cggtggctgg ttgcgtaacg gcattccgca attccggtta ccgcaatcgg tgctggatgc
    46261 agaaatcgcc cgtatagaaa aaatgggcgt gaccattaag tgcaataacg aaatcggtaa
    46321 aacactcacc ctggagcagc tgaaagcgga aaaccgcgcg gtactggtca ccgtagggtt
    46381 atcgagcggt tccgggttat cgctgtttga gcatagcgac gttgagattg ccgttgactt
    46441 cctgcaacgt gcacgacagg cgcaaggcga tatcagcatt caacaaagcg cattaattat
    46501 cggcggcggt gatgtcgcga tggacgtagc cagcacgctg aaagttctcg gctgtcaggc
    46561 ggtaacctgc gtagcgcgtg aagagttaga tgagtttccg gcaagcgaaa aagagtttac
    46621 cagcgccaga gagctgggcg tttcgatcat cgatggattt acaccagtag ccgtggaagg
    46681 caataaagtc acctttaagc atgttcgttt accgggtgaa ctaacgatag cggcggacaa
    46741 aattattctc gctgtaggtc aacatgccga acttgctgct ttttccgcac tggaaccgca
    46801 acgtaacacc atcgaaacgc aacattacca gacccgcgac ccgcaagtct ttgctgctgg
    46861 cgatattgtt gagggtgaca aaaccgtggt ctatgcagtg aaaaccggga aagaagccgc
    46921 cggggcgatt catcactatt tagagggagc ttgctcatgt taacgaaaga tctttcgatt
    46981 actttttgcg gcgtgaagtt tcccaacccg ttctgccttt cttcttcgcc ggtaggcaac
    47041 tgctatgaga tgtgcgccaa agcctacgac acaggttggg gcggcgtggt gtttaaaacg
    47101 atcggttttt ttatcgccaa cgaagtctcg ccgcgttttg atcatctggt gaaagaagat
    47161 accggtttta tcggcttcaa aaatatggag cagattgctg aacatccgtt ggaagagaat
    47221 ctggccgccc tgcgtcggct gaaagaagat tacccggaca aagtattgat cgcttcaatc
    47281 atgggcgaaa atgagcagca atgggaagag ctggcgcgtc tggtgcaaga agctggcgcg
    47341 gatatgatcg agtgtaactt ctcctgtccg caaatgactt ctcatgcgat gggtagcgat
    47401 gtcgggcaaa gcccggagct ggtagaaaaa tattgtcggg cagtaaaacg tggttccacg
    47461 ctgccaatgc tggcgaagat gacgccgaat attggtgata tgtgcgaagt ggcgctggcg
    47521 gcgaagcgcg gcggcgcaga tggcattgcg gcgattaaca ccgttaaatc catcaccaat
    47581 atcgatctta atcagaaaat tggtatgccg atcgttaacg gtaaatcgag tatttccgga
    47641 tattccggta aagcggtaaa accgatcgcc ctacgcttta ttcagcaaat gcgcacccat
    47701 ccagaactgc gcgatttccc gatcagcggt atcggcggca ttgaaacctg ggaagatgcg
    47761 gcggagtttt tattgctcgg ttcagcgacg ttgcaggtga ccaccggcat tatgcagtac
    47821 gggtatcgga tagtggaaga tatggcaagc gggttgtcgc attatctcgc cgatcaaggg
    47881 tttgattcgt tgcaggagat ggtaggtctg gcgaatcaca atattgtccc ggcggaagat
    47941 ttagaccgca gttatattgt ctatccccat atcaatcttg ataaatgtgt tggctgtgga
    48001 cgctgttata tttcctgtta cgacggcggt caccaggcga tggaatggag cgagaaaacc
    48061 cgcacaccgc attgtaatac cgagaaatgc gtgggttgtc tgctttgtgg tcacgtctgc
    48121 ccggtaggtt gtattgatct tggggaagtg aagtttaaga aaggcgagaa agaacacccg
    48181 gtaacgttgt aaaaccagcg acgcatccgg catttagttc ctgtcgccag atgcggcgtg
    48241 aatgccttac ccggcataca aaacaatata aatcagtatg ttgtgctcat tgtaggccgg
    48301 ataaaacgcg ccagcgttgc atccggcatt tggctcctgt cgccggatgc ggcgtaaacg
    48361 ccttatccgg cctacaaaac cacacagatc atgatgttat gtgcgtttat gaacgaatgc
    48421 ggaccacatt cacatcattt cttacgcgcg tatttcagtg aatccagcgc cacggcaaag
    48481 ataataattg cccctttgat gatgtactgc cagtacgggt ttacgccgat gtacgtcagg
    48541 ccatagttga tgacggtaaa aataatcacc ccggtcacca cgccaatcac cgtccctaca
    48601 ccgccgctga acgatacgcc gcctaccacg cacgctgcga tagcatccag ctcatacatg
    48661 aagccgaggt tgttggtggc agagccgata cgtccggctt ctaacatccc gccgaaggca
    48721 tagaacacac cagacaacgc gtagatcatc agcaggttca ggccgacgtt gacaccagat
    48781 actttcgccg cttccgggtt accgccaatg gcaaaaatgt tcttgccgaa gcgggttttg
    48841 ttccataaca cccagacaaa caccaccgca atcaacgcat agaaggtgat gtaagagaga
    48901 cggaaactcc ccagcgcaat aaagccttgg gcaaaagtgg agaaaccact gtcaaaaccg
    48961 gaaattggcg acgccccgac aaagtcgtaa tagagcgagt taatgccgta gacgataatc
    49021 atcgtaccga gcgtggtaat gaacggcgtc acgttgagat aggcgataat caagccgttg
    49081 atcaaaccga tcaccgcacc aatggcacag acaatcagaa taaccagcgc aatcggcatc
    49141 gtcgccattt ccgggaacac tttgttggcg ttatccatgg actgcaataa cgtcgctgct
    49201 actactgccg ccagccccac ctggcgacct gcggaaaggt cagtcccctg ggtgacaatt
    49261 aaccctgcca caccaagcgc gataataata cgcaccgatg actgggtgag aatattactt
    49321 aagttcaaca gacttaaaaa tgttgggtcc tggaaaataa taatcgccag caaaactaaa
    49381 agaacgacgt aaataccgcc ctctttcagg taagtaagaa aacttttctt atttaacgca
    49441 ctcatgggaa gcccctaatc ttaaaggtgc aaagacgcaa gacgcagaat ttcgctttgc
    49501 gttgttgttt ttgtatcgac aattccggaa acgagaccat tgctcatgac cagaatacgg
    49561 tctgttatcc ctaacaactc tggcatttcg gaggagataa taataatccc cttgcctttc
    49621 ttcgccagtt cggcaattaa ctgataaatc tcaaacttag ccccaacatc aataccgcgc
    49681 gtcggttcat cgagcattaa tatttctggt tgcgttaaca gccagcgacc aataatcact
    49741 ttttgctgat taccaccgga gagcgaacca atttgcgtcc gatgccccgg tgtttttacc
    49801 cgcatcgaat caatcaccca ctgggtatcg cttttcatcc gcgagttatc cagtaaacca
    49861 actttatttt tatagttgcg gatattggaa attaacgagt taaagccaat atccagatag
    49921 gcataaattc ccgttgagcg gcgctcttca gttaccagtg caaatccgtg gtttatggct
    49981 tcgttggcat tatggttatt gatctttttg ccgtgcaacg taatggtgcc agccgatttc
    50041 tcgcgaatac caaataacgt ctcaacaata tcggtacgtt tcgctcccac cagaccggca
    50101 ataccgagga tctccccttt atgcagatca aacgagacat cgcgaatcga cggctggcgc
    50161 agcgacgtca ggttacgcac ttccagaatc acttcgcccg gcttgttttc tttgtcaggg
    50221 aaacgctggt taagagaacg cccaaccatc atggcgatga ttttgtccat cgtcagtcct
    50281 gccagcggtt cggtagcgat ccactgaccg tcgcgcaata cggtaacttc atcacataac
    50341 tggaagattt cttccatttt atgcgagata taaacaatac cgcagccgcg ctcttttaat
    50401 ttacgaataa tagtgaacag atgattgacc tctttttcgg ttaacgaaga agtcggttca
    50461 tccataatca caattttcgc gttataggaa aacgctttgg cgatttcgat catctgcatt
    50521 tgcgaaacag ataatgtacc gacgcgcgca cgcggatcga tatcaatatc cagttcatca
    50581 aaaatcgctt tggtttcgcg gtacattttg tcctgatcga caaacatgcc tttggtggga
    50641 tatcgcccca gccacatgtt gtccatcacc gaacgttgta ataccaggtt taactcctga
    50701 tgtaccatcg aaataccatt ttccagtgct tctttggcgg aatggaaatc gatctcttta
    50761 ccctggaata aaatggtgcc ggagtctttt tgataaatac caaacaggca ttttaataat
    50821 gtcgattttc ctgcaccgtt ttcccccatt aatgcatgga tagaatgtgg ccggactttt
    50881 aagttaacgt tatcaagtgc cttaacacca ggaaaagact tgttgatacc gctcatttcc
    50941 aacaagtatt cacctgatga cggagtcgtt gagctgacca tataatttta ccttgttggt
    51001 catacaataa gggcgcagta aacgactgcg cccaatcagt cttatttctt gctgaattca
    51061 gccaggttgt ctttatctac gccaacataa ggtacgcgga ccactttgtt gtcgattttc
    51121 cagttggtgc catcagccgc acctttgcca tcggccaggt ttttcgccag atcaaaggtc
    51181 gctttcgcct ggttgttagc atcgttcagt acggtgcccg ccagtgcacc ggatttcacc
    51241 agcgccagcg cttctggcag cgcatcgacg ccaaacaccg gaatgctgga cttgttgtgt
    51301 gctttcagtg cttctaccgc gcccattgcc atcgcgtcgt tgttggcgat aaccacctcg
    51361 attttgttgg cgttcgggcc agacagccag gcgtccatct tatctttcgc ctgagcggta
    51421 tcccacatag cggtatctaa ctgtaactgt tcagttttaa tgcctttgtc gttcaattct
    51481 ttaatcacgt aagtggtacg tgcttctgca tccggatgcc ccggctcacc tttcagcagt
    51541 acgaattgaa tctgaccgtc tttgttcaga tcccaaccct gattcgccgc ccagtgttta
    51601 gcaatcaaat cgccctgaat aatgccggac tctttggagt cagtgccaac gtagtaggct
    51661 ttgtcgtagc tatccagcgc cttacgagac ggttctttgt tgaagaaaac caccggcacg
    51721 ttttgcccac gcgctttctc aatcaccgta cccgcagctg ccgggtcaac caggttgatt
    51781 gccagtgcct tcaccccttt cgccagcaat acgtcgatct gatcgttctg cttggactgg
    51841 tcattctgag aatcattcat cagcagctga acatctggcg cggctttcgc atcttgctca
    51901 atagccttgc gcactacaga cataaagtta tcgtcgtact tatagattgt tacaccaatg
    51961 cgagtatcag cagcgtgtgc agcggcaccg aataacatgc tggccatcac agcagacagg
    52021 gttaacacct tcttattcat ggtatctccg gtttttctta tgcagggtag tgcttgagat
    52081 aaatgctcgg cggggcagta gagttaatga agtgttactg aacgccgaag ctcacttttt
    52141 aaaattcgtt cttccatgcc cggtaacgct ccagaaaacg gctttaattg ttgttatgac
    52201 gctattacct cggtgaaagt gattattcac tgttacatat gggttacaac gttaaaacgg
    52261 tgcaatcata gccatcacat tgttaagata ctgtgaaatc actcacagat tgaaagcggt
    52321 tacatcgcct gatttgttga gttagtgatc gccgccgcat tctggcgtgt tgcgacagaa
    52381 tggcgacgca ctaacgtcgg cataaaacag tggctggcac gaggatcaat attgtctgct
    52441 gccccctgca aggccagttc ggtagctaat ttagccattg aagcaatggg ataacgcacg
    52501 gtcgttaatt gcgggtcggt gtaacgggcg atgggaatat catcgaaacc gatgattgag
    52561 agatgtagcg gaatcgcaat gccattatct tttaatgctg tcagtgcgcc agcagccata
    52621 ttgtcgttat aagcaaatac agcggttagt tgtagattgc gccccagcag ttcaaccatc
    52681 gccgcctcac cgcccggcat gtccggcgta ccagtgccaa tccagctttc cggcggaata
    52741 atatcctgct ctttcaacgc actcatccag cctgctttac gcatggcgtc atcttcaatg
    52801 ccgtggctgg aagaaagata accaatacgt tgatgaccgt tattcagcaa catgcgcgtc
    52861 gccattcggg caccgctgag attatccagg caaacgcaac gatgggcgta ccccggcaca
    52921 acgcggttga ttaacaccat accaggaatg ttatccataa attgtgccag ttcatcgtca
    52981 ctcaatgctt ttgagtgaac aatcaacgca ttacaacgct ggcgaattaa cacctcaatg
    53041 gcgtgacgct ctttttccgc ttcatgatag ctattgccga ttagcacgta tttctgatgc
    53101 tgctgggcga ccagatccac cgcttttacc agcgcgccga aaaaagcatc agaaacgtcc
    53161 atcaccacca cgccaatggt gtcgctaacc tgagttgcca gcgcctgggc attggcgttt
    53221 ggccgataat ccagctcact cacagctttc attactgctt cacgcgtgtc ggcactgacc
    53281 agcgtgctgt tattgagcac ccgggaaacc gttgccacag agacgccagc ctgacgcgct
    53341 acatcacgaa tggtgatcat attcactacc tgttatgaga ttgcagtaaa tgactgcttg
    53401 ctggcggcta ttttgtcagc actgaataca ggacttcgtg aatcgagtca cagcaatgga
    53461 aacggttaca gccgttttgt taacgaatgt gatccagatc gttatcttga tgttttagat
    53521 attgtcggtc cggcagcacg caaagttaac tgacgccaga gccattccac cggcccctgg
    53581 cggaagtaac gcagccagat aacagagaag aagatattcg ccagccatac cggaataaca
    53641 aacgccagca gctccagacg gtcaaactgc ataaacaaac tgaggtggta aaaaagcgta
    53701 gtacaaatca gcgtttgcaa tagatagttg gtcagtgcca tccgtccgac acaggcgatt
    53761 gccagcacca gcttaaagcg gcttaattgc ggccagaagc cataaaacag cgacgcatag
    53821 ccaatcgcct gaaacggcgc actcagttcc cgcggcattt gaagtaagaa ggcacaccag
    53881 cgatacgccc agtccagctg ccattgcagg gcgatggcag gaaggttaat gatcacccca
    53941 atcgccacca gcacaaaacc agtacgacga tagtgacgta agctgaactg ccctttcagc
    54001 cagccgctgc gcatcagcgc cgcaccaatg agcatcatcc ctgccagttg ccagccatac
    54061 tgtgcgccca gtgccagtaa actgttgcca acaccatcgg cacgattatt gatcgcttca
    54121 acaccaccgt gaagcttcca gtatttttca tacaaaatag ccgatgcatc cggcgtccag
    54181 gcgcggctgg tctggctgtc ggaaatcagc cccaataaca gcaaaacgcc aagccccacc
    54241 agataaagca tgacgcctgt attaaacagg ctttttaccg atggcgcatc gcgcaccagc
    54301 cgccagcaga ttaagcccac cagtccgtaa gccagcagaa tatcgccgtc ccagaacaat
    54361 aaaccgtgaa taaagcccag caagactaac agcgttaacc gcgactggat ccagcgtctg
    54421 ccacggggca gcaacatttg caggcccgca ccaaacagca gcgcaaaaag cgtgaggaat
    54481 ttcacctggc cgatgagatc gagaaatgcc caggtccatg catcctgcgg cgtaatagcg
    54541 ccgtaccagg cgggattgag ataagccgcc tttggtagcc caaaggcgct gatgtttaat
    54601 agcaggatcc ccagaatggc gacgccgcga acaaaatcga gcgtgacgtt gcgctccatg
    54661 gctcctgcct tttaatcagt tgtgatgacg cacagcgcgc agaaactcgt ggcgcgtatt
    54721 ctgactggat ttgaacaatc caccaagaga ggtcgttgtt gtggcactgg ttgcgtcgcg
    54781 gatgccacgc gctttcacgc agtaatgcac cgcgtcgata gagacagcca cgttattggt
    54841 gcccagcagc gtttgtagcg caatgagaat ttgctgcgtc agacgttcct gcacctgcgg
    54901 acgctgggca aagaactgca cgatgcggtt aatttttgac agaccgatca ccgaatcttt
    54961 cgggatataa gccaccgtcg ctttgccatc tatggtaaca aaatggtgtt cacaggtgct
    55021 ggtcagggtg atatcgcgca cggtgaccat ttcatcgacc ttcattttgt tttcaatgag
    55081 ggtgattttc gggaaattgg cgtaatccag accggagaaa atttcatcga catacatttt
    55141 agcgatgcga tgcggcgttt ccatcaaact gtcatcagcc aggtcgagat tcagcagctg
    55201 catgatttcg gtcatatgac cagcaataag gcttttgcgc gtttcgttat ccatttcatg
    55261 cacgggcggg cgcagcggtg tttccagtcc tcgcgcaact aacgcttcat gaaccagggc
    55321 cgcttcttta ctgagtgatg gcatttatga tttctcctgc aggtgtgacg cctccgccct
    55381 gcgtgggggc aaagttatta agctgattta cagcctgatt attgtgcgtg aggcggcgca
    55441 cataatccag tattcacagc gataattatt gtaattgccg ctgcctttca tcagcagatg
    55501 ttaaaacatc gttatgcaaa tacggaagtg aaagttactc acagcacatt gaataaacgg
    55561 tatgatgaag aaattgcaaa caacacaaca aggagccacg catggaaatg ctcgaagagc
    55621 accgctgttt tgaaggctgg cagcaacgct ggcgacacga ctccagtacc ttaaactgcc
    55681 cgatgacgtt cagtatcttt ctcccaccac ctcgtgatca caccccgcca ccagtgctgt
    55741 actggctttc cggattaacc tgcaatgacg agaacttcac caccaaggcg ggtgcccagc
    55801 gcgtggcggc ggaactgggg attgtactgg taatgccaga caccagcccg cgcggcgaac
    55861 aggttgccaa cgacgatggc tacgatttag gccagggcgc ggggttttat cttaacgcca
    55921 cgcaaccacc gtgggcgacg cattaccaga tgtatgatta tctgcgcgat gaattaccgg
    55981 cactgattca gtcgcaattt aatgtcagcg accgctgcgc tattagcggt cattccatgg
    56041 gtggtcacgg cgcgctgatt atggcgctga aaaatccggg taaatacacc agcgtttccg
    56101 cctttgcgcc aattgtgaat ccgtgcagcg tcccgtgggg aatcaaagcg tttagcactt
    56161 atttaggtga ggacaaaaat gcatggctgg aatgggacag ttgcgcgttg atgtatgcca
    56221 gtaacgcgca ggatgcaatc ccgacgctta ttgatcaggg tgataatgat cagtttcttg
    56281 ctgaccaact gcaacctgca gtactggcag aagccgcgcg ccagaaagcg tggccgatga
    56341 cgctgcgtat tcagccggga tatgatcaca gttactactt catcgcctct tttatagagg
    56401 atcacctgcg cttccatgcg cagtatttac tgaagtgaaa gtccgcccgg ttcgccgggc
    56461 atcttctcat cagaagcgat aatccactgc cataaagtaa cgacgtccgt cttcgttata
    56521 gctgtagtcg tcacgactga gatccttgtc gccaaggttc agcacgcctg cacgcagttt
    56581 gacgtcttta gtcacctgcc aggccgcgcc ggtattccag atggtataac cgcccggtgt
    56641 tttagccgtc gcgctgtcgg cgcgtttctg cccggtatag tgaccagaaa catagaatga
    56701 ccagtcttcc agcgccagcg gtttccagtc cagcgtaccg ttagcagtat ggaacggcag
    56761 atcggatagc ggtttgtttt cgccgttgct gacatcacga ccatcgttgt aggtgtagtt
    56821 gatcgacagt ttccattcat cgttgaacgg aattttcagt tcggtttcca cgccctgaat
    56881 acgtgctttg ttaacgttgt agtagctaaa taccggtatg cgccgtccgt tagcgcccgt
    56941 ctcaaaaccg acaaagtttt ggtagcccgg tgcagcgttg acgtcagacg tacggctaat
    57001 gctgatacga tctttcacat cgttacggaa aacggtaacg ctggattcaa ccccttccag
    57061 ccagccttct tcgcccatgt agtaaagccc cagttcccaa ctttcgctgg tttctggttt
    57121 cagatccggg ctacccacaa tcttacatgc gccacggcag gaattgctcg tccagtcagg
    57181 gctaagttgc aacagagaag gtgctttaaa tgccgtcgcc cagccccctt tcaccgttac
    57241 ggtgtcggtg gcgttataaa ccaggtaggc acgcggactc cagtgttcac cgtaggtttc
    57301 gtgatcgtcc atacgcacgc cggtcgtcag cgccagcggc tcgaagatcc gccattcatc
    57361 ttccacaaac agcgcgtact ggctggcaga cgttttggag ctggttcccc cggtcaggtt
    57421 caccgcatcg ctaagtttgt cgtgacgcca ttcaccgcca accgtgagaa actgattaat
    57481 cgccgtcagc ggcaacgtgt atttgccgtc gaccgtattg ctttcggaag ttatcgggct
    57541 gctgttgcca gggtttttgt tctcgacttt ctcaccgtag tatttcagtt cgctggtgcc
    57601 gtaatcccaa cgcccattat ggctgacgga gtagttctgg cgttccaggc ggtttttgtc
    57661 cagcgagtcg gaatcacgat cctgacggtc gaaaccgtat ccggcagtaa aatcgtgatt
    57721 ttgattcggt gtccaggcaa attcgacatt gccgtcgcgg ctggagaatc cttcaatacg
    57781 cggcgtttct ccggtatcgg tggtcgttga gttttgcggg tcatcctttt cacgttttgc
    57841 caggctgccg taagctttca ttcccagcac gccgtcaatt aatggtccgc tggtaaagaa
    57901 ctgaccgtta taggtatcac cgcgatcgcg atgttcctga acggtggtat cgacggtaac
    57961 ggtgcccgac catttctgac cgattttttt ggtgatgata ttcactacac cgccgagcgc
    58021 atcggaaccg tacagcgacg acatcgggcc acggaccact tcaatacgtt cgatggaatc
    58081 gaccgggatc cagttcagat cgaaatcatt gtggcggaag acggcattgc gggagttaac
    58141 gcgtttaccg tcgacaagaa tcagggtgta gctgctgtcc agaccacgaa tacttacgcc
    58201 cttacggtta tccccttcgt tcgtcagttg tacgccaggc acttctttga ggacatcctt
    58261 cagattctgt accggttttc gctgcaggtc ttcctgggta atgacgctga tactggcggg
    58321 agcatcttta aggttttgtt caacggaaga tgcagtgaca accatcgttt cgccatcatc
    58381 atcgaccgct aacacaggcc atgcacaaga aatagcggac aaacacagcc cgacccgtac
    58441 gaaagggttc aacctaaaca ttccatatct ccatgaggta actacgaaaa taaaatgggt
    58501 atcgctcaca tcttctttac gtccccttgc gtacggcagc atcgcggtgg acttatttgt
    58561 atgcaggtga tcatcccaga aagccggaac agcctctgat ttgtcagcct tttttgattg
    58621 cggcgtaacg ataatgcaaa cgataacaat tatcaatcca aatgttaaat tttatatccg
    58681 catgatttgt cagggaaatg gaattgaaaa agccctctcg gttgagaggg cttagcaagg
    58741 aagggaggaa acttatttct tatcgttctg cgggaacttc atttcgctgt agcgtacgaa
    58801 gtgagttcct ttaatcagct tatagccgaa ccaaataatc aggaacagcg ggataccaat
    58861 atacgttgcc gctacgccgc cccagtcaat ggtatctttc aggaacgctt cgtagttctg
    58921 gcccaaagtg ataatcagac acagaatgaa tgcgaagatc ggccccagtg ggaagaaacc
    58981 tgaacggtac ggcagatcgt taatgtcgtg tccctgcaat acgtacccgc gacggaaacg
    59041 atagtggcta atggcaatcc ccagccaggc gataaaaccc gtcatcccgg aggtgttcag
    59101 cagccacagg tataccgtct ggttgccaaa catggaggtc aggaagcaca aaccggcaat
    59161 caccgtcgtc gcatacagcg cattacgcgg cacgccacca cgcgacagtt tagcgaaaat
    59221 gcgcggcgct ttaccgtcac acgccagggt gtacagcata cgagtagacg catacatacc
    59281 ggagttaccc gctgacagca ccgccgtcag aataactgcg ttcatcaccg ccgccgcaga
    59341 gagcagaccc gcgtgctgga acaccagggt gaacggactg acgctgatgt ctttaacatc
    59401 gttacgcagc aggctcggat cggtgtacgg aataatcagg ctgataatca ggatcgcgaa
    59461 cacatagaac aacaggattc gccagaatac ctgacgtacc gcgcgtggaa tgtttttcgc
    59521 cggatcttcg gactcgcctg cagcaatacc gatcagctcg gttccctgga aagagaagcc
    59581 gacaatcata gctacgccga tcatcgccgc aaaaccacca gcaaacggcg cttcgccgat
    59641 tgtccagttg ctccagcccg caggctgcgc gcctttgaag ataccgataa tcatcagcac
    59701 gccaacgatg ataaagacaa taactgtcgt gactttgatc agtgagaacc agtattccgc
    59761 ttcaccaaag ccgcgaacag agatgtagtt caacaggaag ataacgccga ggaacaacgc
    59821 actccagatc cagcccggtg tatccgggaa ccaccagctc atgaccagct gtgctgcaac
    59881 caggtcaacg gcaatagtca ccgcccagtt gtaccagtag ttccagccca gcgcgaagcc
    59941 aaagccttct tcaacatagt tctgaccgta cgtggcaaac gaaccggaaa ccggcatata
    60001 tgcagccagt tcaccgagac tggtcatcag gaagtaaacc atcaggccaa tcagcatata
    60061 cgagagcaat gccccgcccg gacctgcctg agaaatcgtt gcgccagagg caacaaaaag
    60121 acctgtaccg atggaaccgc caatggcaat catcgtcagg tgacgcgcct tcaattcacg
    60181 gcgtaagccc ggcgcttctg tagttttagt ttcggaaccc atacaaaaat gctatccatc
    60241 ttaaaaatga ggcgcgattg tagcagacga ttggcaatcc ttccggcaga aatacacggt
    60301 tataagacac cttcatgatc gcccagggat tataagtaaa gcagccaatc tcttttctgg
    60361 cgaatgcatg ctgagggcgc tgcgtcacga aatcgacaca gcaccagcat gttcttgtac
    60421 agcaccttaa cgcggtacat tcgcgggatc gcaatagtcc agaaagcgcc gtagcgcgtt
    60481 ggaaaggtgt ttttgccgat gatgtatacg ccacaacgta cgcatcaggc gcggcagagg
    60541 gaccgcaact tcacttaatg tgcctgcctg caattgatct tcaatcaccc gtcgcgacag
    60601 gcaactaatt cccaacccat gacgcaccgc atgtttgatt gcctcggagt tacctaattc
    60661 catcgccatc tcaaacttcg gtaaatgtga cagcaacaga taatcgacaa tctcccgcgt
    60721 gccggaaccg cgttcacgca ggatccacgg cgcagcggcc agctgttcta aggtgaccgg
    60781 accacgagcc aacggcgaag tcggcgcggc gaaaaccacc agctcgtctt ccagccacgg
    60841 ttcagaaatg atttcagtgc tgtggcacgg tccttcaata aagccaatat caacgcggaa
    60901 atccagcacc gcttgcatca cgtcctggct attcccaacg ctaagttcaa tcggcaactg
    60961 cggataatca tggcgataac gggcgataac tgcaggtaga atgtagttac cgatggtact
    61021 actggcatag atacgaatcg cgccgttgtc ttcgcgaaac agttgttcga tttcaaccgc
    61081 ctgttcaagc agtgccaacg ctcgcggata gagcagccgc ccgtgttcat taacaaccag
    61141 tcttttcccc acgcgatcaa acagttgaac gccaagctgc ccttccaggt cggtcaaggc
    61201 tgcgctcact gctgattgcg acaacgccag catcaccgac gcctgggtgg ttgatccact
    61261 tttcaatact tctgcaaaaa cttccaactg ccggagggtg atgtgcatag tcgcttacca
    61321 cttataaaga ttaattataa atatataatc aattttattt ttaaaccagt tagtcgtaac
    61381 cttatgcccg gttaaaggag agggttatga cgaatatcac cttacagaaa caacatcgta
    61441 cactgtggca ttttattccg gggttagccc tgagtgcagt tatcaccggg gtcgccctgt
    61501 ggggtggttc cattcccgcg gttgcgggtg ccgggtttag tgccctcacc ctcgcaatct
    61561 tgttggggat ggttttaggc aacaccattt acccgcacat ctggaaaagc tgtgacggtg
    61621 gtgtgctgtt tgccaagcaa tatttattac gtctgggtat cattctttat ggcttccgtc
    61681 tgacgttctc gcaaattgcc gatgtcggta tcagtgggat catcattgac gtgttgacgc
    61741 tgtccagtac cttcctgctg gcttgcttcc tggggcagaa agtgtttggt ctggataagc
    61801 acaccagctg gttgatcggt gccggtagca gtatctgtgg tgctgccgcg gtactggcga
    61861 ctgagccggt agtgaaagca gaagccagta aagtaaccgt ggctgttgca accgttgtta
    61921 tcttcgggac cgtcgcgatt ttcctctacc cggcgatata tccgctgatg tctcaatggt
    61981 ttagtccgga aactttcggt atctacattg gttctactgt gcacgaagtg gcgcaggtgg
    62041 tggcggcagg tcatgccatc agcccggatg cagaaaacgc agcagttatt tccaaaatgc
    62101 tgcgcgtgat gatgctggct ccgttcctca tcctgctggc ggcgcgtgtt aaacagctgt
    62161 ctgggacgaa cagcggcgag aaaagcaaaa tcactattcc gtggtttgcc atcttgttca
    62221 tcgtcgttgc catctttaac tcgttccacc tgttaccgca gagcgtggtg aacatgctgg
    62281 taacgctgga taccttcctg ctggcaatgg cgatggcggc gctgggtctg accactcacg
    62341 tcagcgcgct gaaaaaagcc ggggcgaaac ctctgctgat ggcactggta ctgtttgcct
    62401 ggctgattgt tggtggtggt gcgattaact atgtgattca aagcgtcatc gcataaacca
    62461 ctacatcttg ctcctgttaa cccgctatca ttaccgtttt cctccagcgg gtttaacagg
    62521 agtcctcgca tgaaatacat tggagcgcac gttagtgctg ctggcggtct ggcaaatgcc
    62581 gcaattcgcg ccgccgaaat cgacgcaacc gcgtttgcct tgttcaccaa aaaccaacgt
    62641 cagtggcgtg ccgcaccgct cacgacgcaa accatcgatg aattcaaagc cgcctgtgaa
    62701 aaatatcact acacatcggc gcaaattctt ccccacgaca gttatctgat taacctcgga
    62761 catccggttg ctgaagctct ggaaaaatcg cgcgatgcct ttatagatga aatgcagcgt
    62821 tgcgaacagc tggggctttc tttgctcaac ttccaccctg gcagccatct gatgcagatt
    62881 tcagaagagg attgccttgc gcgtattgcc gaatccatca acattgcgct ggataaaact
    62941 caaggtgtca ccgcggtgat tgaaaacacc gccggtcagg gcagtaactt agggtttaaa
    63001 ttcgaacatc tcgcggcgat tatcgacggc gtggaagata aatcccgcgt cggcgtctgc
    63061 attgatacct gccatgcttt cgctgccggg tatgatttgc gtactcctgc cgaatgcgag
    63121 aaaacgttcg ctgattttgc ccgtattgta ggctttaagt atctgcgcgg gatgcacctc
    63181 aacgatgcga aaagcacctt tggcagccgc gttgaccgtc atcatagcct cggcgaaggc
    63241 aatatcggtc atgatgcgtt ccgctggatc atgcaggacg accgtttcga cggcattccg
    63301 ctgatccttg aaaccatcaa cccggatatc tgggcagaag agatcgcctg gctgaaagcg
    63361 caacaaactg aaaaagcggt ggcctgatcg atgaacgaca gggaaaaaca gattcttaaa
    63421 attttacggc gtaacccgct gattcaacag aatgaaatcg ccgatattct gcaaatcagc
    63481 cgctcacgtg ttgccgctca tattatggat ttgatgcgca agggtttgat taaaggcaaa
    63541 ggatatatcc ttaccgagca ggattactgt gtggttgtgg gtgcaatcaa tatggatatt
    63601 cgcggaatgg cggatatccg ttacccgcaa gcagcctctc atccaggttc tgttcactgt
    63661 tccgcaggcg gcgtcggacg taatatcgcg cataacctgg ctctgcttgg tcgggatgtg
    63721 catttaattt cagctattgg caacgatttt tacggcgaga cattactgga agaaacgcgc
    63781 cgcgcgggcg tgaacgtttc aaactgcatt cgtctgcatg gacacagcac tgcaacttat
    63841 ctcgctatcg ctaataagca ggaagaaact atcctggcaa tcaatgacac acacattctg
    63901 caacagctaa cgcctcaact gctcaatact tcacgggact tgattcgcca tgctggtgtg
    63961 gtgctggctg attgtaatct cacacctgaa gccctggagt gggtctttac cattgctgat
    64021 gaaattccga tgtttgtcga taccgtttcc gaatttaaag cgaacaaagt aaaaaactgg
    64081 tacagccgga tccacactct gaaaccaaca caaaatgaac tggaaatttt gtgggggcaa
    64141 ccgattaaag acgacaacga tcgcatcaga gctgtaaatt cgctgcatca acaaggtgtt
    64201 aaacgcattt ttgtttatct gaaagatgaa tctgtatttt gcagcgataa ggatggagaa
    64261 caatttttgc tgaccgcacc agcgcatacg acagtagaca gttttggtgc tgacgatggt
    64321 tttatggcgg gcctggtata tagctttctt gaaggataca gtttccgcga cagcgcccgt
    64381 tttgcgatag cctgcgcggc aatttcgcgt gccagcggca gcttaaacaa ccctaccctg
    64441 tctgccgata acgcgctttc attagtgcca atggtgtaac aatgttgccg gattcgacgc
    64501 ttgaccgcat cttatccggc tcataaattg cgcactacgc taaaccaata aagaatcctg
    64561 caatagtagc actcatcagg ttactgacag caaaaacgca atagccagca ataccaccat
    64621 tcccagaaca cttctcatga catccatagt tattatctct tcatgtcatt ttgggcgacg
    64681 gctctctgcc catcataaat agccgggcct gcatcttgcg atactgaccc ggcttgttat
    64741 caacacttaa tgggttttga tgtagccgcg tacgcactct tcgactaatc cccagaacca
    64801 gtctgtatca atagtgatgc cgactttggt attggcgggc ttgccaagaa cgcccaactc
    64861 gtcgcagacg gtacgcccat agcaagggcc actgttaacg tccacttcga cgtacatctc
    64921 ctgggtttta atgccatcag ggttaatcaa ataaccgatg caggtggcgt cgtgcaccgg
    64981 gccgccagcc aggccgtagt tttcgaactg cgttttgaga gtgaagttca tgatgtcgct
    65041 gaacagctct cccgcagggc cgccagccct ttccatccga gcaatcacgt ccggggtgca
    65101 aacggtctgg ttggtaagat cgaggcccat catcactaat ggaacgccgg aggtgaacac
    65161 cacgcgtgcg gcttccgggt cagcaaagat attgaactca gcagatgggg tgaagttgcc
    65221 agtaccataa gcgccgccca tcagaacaat ttcacggatt ttcggcagga tcgcgggttg
    65281 catacgcatt gccaccgcga tgtttgacaa cggaccaacc ggcaccagag tgatatcgcc
    65341 atcgcttgcc ataagggtat cgatgatata tttcaccgca tgagtgtttt ctgcctggcg
    65401 ggtcagcggc tcgaataccg ggccatccag tccggtttca ccgtgaatat tatcggcaac
    65461 aatttgttga cgcataatgg gctgcggcat ccccgcataa accggaacat taatctccag
    65521 tttctggcaa acattcaggc cattaattaa tgttttatcg agggtttgat tacccgctac
    65581 aatggtgata cctaataaat caatcgcagg atgtttcgcc gccatcatta tagcaatagc
    65641 atcatcatga cccggatcac aatccagaat aatttttctc ttttccattg tttatttcct
    65701 ctgtttccag ttgcgttatt gtttctacag caaagaaaat tcgacctggc cgatgattga
    65761 atcttaacaa cagcgtacgt atgctaaata tgagaaatct catagcggat aaacatcgtg
    65821 aaagaaatcc acaataatga tcttaagcag caattgatga gtgaatctgc gtttaaggat
    65881 tgcttttcaa cggatgtttc agccgatacg cggctgtttc attttttagc gcgtgactac
    65941 attgtgcagg aagggcaaca gccgtcctgg ctgttttacc tgacgcgagg ccgcgccagg
    66001 ctttacgtca cgcttgctaa tggtcgcgtg tcgctgatcg atttctttgc cgcgccctgt
    66061 tttattggcg agattgagtt aatcgacaaa gaccatgaac cgcgtgcagt gcaggctatt
    66121 gaagagtgtt ggtgccttgc gctccctatg aagcattacc gcccgctgtt attaaacgac
    66181 acgctatttt tacgaaaact ctgcgtcacc ttaagtcata aaaattatcg taatattgtt
    66241 tctttaactc agaatcaatc atttccgtta gtgaatcgcc tggcagcttt tatattactc
    66301 tcgcaggaag gtgatcttta tcacgaaaag catacgcaag cggcagagta tttaggcgtt
    66361 tcttatcgac atcttttata tgttctcgcg caattcatta atgacggttt attaattaaa
    66421 agcaagaaag ggtatctcat taaaaacaga aagcagttgt caggactggc gctggagatg
    66481 gacccggaga ataaattctc cgggatgatg cagtaaaaat tattggcaat agtgcgattg
    66541 ccggatgcaa cgcttaataa ggcgtacatc cggcaatgaa actcaagcta aaccaataaa
    66601 gaacccggca atggtcgcgc tcatcaggtt ggaaagcgtc gccgccgcca gcgcccgtaa
    66661 accaagctgg gcgatttccg gcgcgcgttt tggcgaaata gccgaaaacg ctccaacgac
    66721 aacaccgata gaaccaaagt tagcaaaccc acagagggcg aacgagataa tggcaatggt
    66781 cttgacatcc agcgtgccgc tcgtttgcag atagggcgag aaattgagat aagcaacaaa
    66841 ctcattaatc gccaatttct gcccaatcaa acttccggca agatttgcat cactccagtc
    66901 cacccccata atccatgcca acggggccag cacatagccg aaaatgcttt ccagagagac
    66961 attggcaaaa ccaaaccagc cgccaactcc accaataatg ccgttgatca gcgcaatgat
    67021 cgccacaaat gccattacca ccgtcgccac accggcggcg attttcagcc cggtcattgc
    67081 accgttcgcc gcagcttcaa taatgctttt tggcggtgtt tcgctaaacg acagattttc
    67141 aaacgtaact tgcgaaggtt ccgtcgccgg actaagaata cgtgcaaaca ggataccgcc
    67201 aggaatagcc atcagcgaag cggctaacaa atagtcaatc ggcacgccca ttccggcata
    67261 gccaatcatc atcgaacccg caatagacgc catgccgcta caaatagccg taaatagctc
    67321 gttgcgattc atgcgatcaa taaaaggttt aacgatcgcc gggatctcat tttgcccgag
    67381 gaagatagtg gtgactgcga caaaagattc gattttgctg atgttgaggg ctttctgaaa
    67441 aatactgcca agaatacgaa tcagcagccc cattacgcca atgtagtaca gcaagctgat
    67501 aagcgcggta acgaaaataa tcgccgggag tacgcgaaag gcgaagataa agcccgcacc
    67561 gtcaaacaga acatccattt tgggaccgac cagcgaacca aaaataaagg cacttcctgc
    67621 gtcactgtag gacatcactt tatggacgcc taatgccgcc tgctctactg cccattttcc
    67681 cgggggaaaa tagagcatga tgccaccaat ggcaatttgc agcagcagcg cagccccaac
    67741 cgtgcgcaag ctgatgctct ttttatttac cgacaacaca aaggcaattg ccagcaatac
    67801 caccatcccc acaacacttc tcattatatc cataatgatt ttcccttcat gccagtaaac
    67861 ccggcgtcag cgccaggttt tggtatgttt gatgagtacg ggcgacggct ttctgcccac
    67921 cagaaattaa cccacgagac gctgatattc tttggcaatt tcgctcgcca gaatggcgtt
    67981 attaaagacc agttgaatat tggatttcag gctatcaccg ccggttagct ccgcaacacg
    68041 cgccagcagg aacggtgtac tctctttccc aataacaccc tgttcttcgg cttcagcaac
    68101 ggcttgatca attgctgcat tgatggtttc ttctggcatc gcaaactgtt ccgggatcgg
    68161 gttcgctacc accaggccgc cgttcaaacc ggtttgccac ttcactgcca tcgcgcgagc
    68221 aatttcgcgg gcactgtcga gacgaatgct gacatcaaat gggctggttc ggcagaaaaa
    68281 cgcaggcagc gcttttgtct gataaccaat taacggtaca ccgaaggttt ctaaatattc
    68341 tgttgttaat cctaaatcga gaatagattt tgcccccgca caaacaacag tgacattggt
    68401 atttgccagt tcctgtaaat cggcagaaat atcgaaggta tgttccgctc cgcgatgcac
    68461 accaccaata cctccggttg caaaaacttt aatcccagcc agtgctgcaa taatcatggt
    68521 tgatgcaacg gtagtcgcgc catttttccc tgcagcaacg acaaaaggta aatcacgacg
    68581 actaactttc gtcacgttat gtccttcacg gcccagcaat tcgatttctt ctttacttaa
    68641 gcccactttc atcacaccgc caataatggc gatagtggct ggtactgcgc cctgtttacg
    68701 aattgtttct tctacttcaa ttgcggtttg ggcattttgt gggaatggca ttccgtgaga
    68761 aattatggtc gattccagcg caacaaccgg ttttttattt tttaaagcat cctgtacttc
    68821 agcggatatt tgtaataatt caggggacag agttaattca gacattctgt gttctccact
    68881 aacgatgtaa cattggcaat tgataattca gggttgttgg tatattcaca ggcaagtgcc
    68941 attgacgaac atccctgcgc aaatcgaaca gaatcgataa acggcatacc gtctacccaa
    69001 caggaggcca gccccgccat catggcgtcg cctgccccag taacattgat gacattcgtt
    69061 ttaatagggg ccgaccaccc actttcaccg ttgatatcac tgtagtaaac gccatcccct
    69121 cccatgctca gcaccaggcg gttcaggccg tgttgatgaa accaggcggc gacctttgcg
    69181 acatcttcac gtccggaaag tgcaattccg ctcaacgttt cggcttcaag acggttaggt
    69241 tttaacgtat ggattttgct cagatgatcg cggattttga cgcatttcca tgccgatacc
    69301 ggatcaataa atacaggcgt ttccccgcta ttttccagaa cccagaccag cgcctcttca
    69361 ctaaggttgc aatccgcgac aataaccttt gcaccgcgaa taaactcctg atgctgggca
    69421 agaaattctg cagaaataca gtcactaata ctcatatcat ttatcgcgac aagcatttct
    69481 cctgtattat cgagcagtga taaatagctg gatgtattct cacccggaac aattaagcat
    69541 ttatcgacat aaacaccgga ttgattagtt tgcgccagta atgattgccc ataaaaatca
    69601 ccacctactg cacttaaaag ccaggcattt ttccccagta acgccagatt atgagcgata
    69661 ttccttccca cgccacctgg ggtaaattta atttttccgg gattcgaatc cgcataattc
    69721 aatggatgat gtgaataccc agccacatca atattcgccg agccaataat tactatgtag
    69781 tccttttcgc gcatagcacg ccccctggca ataaattgcc aacgaagaaa ttgggtaaaa
    69841 tagtcattac agtcaataag actattagag catgtgttca gatttaaaca catgttcata
    69901 ataagcatgt ttaacgtaat gtaaatcgat cagaaaaaac gaaatcggcc agatatgaag
    69961 gcggtcacaa aatttgtcta tttatgcagc aacaaaagag aaagttgtga tgtgtaacac
    70021 acaatatggc aggcaagaaa aagggcagag atgtttctgc cctcagattg ttgacaatgc
    70081 gcgctttgtt catgctggat gtggcgtaaa cgccttatcc gacctacaaa agcgcaaaaa
    70141 ttcaatatat tgcagggttc atgtaggtct gataagcata gcgcatcagg caattttgcg
    70201 tttgtcatca tcggggcaga gatgtctcat ccccgatgat gaagagaaat tatgcgtttt
    70261 tttctactat ttgtgtttcc gggcgtttga ggaaggcata ggccaaaccc gccaccagcg
    70321 taccggcaat aattgctacc aggtaaccca ataccggcgt aatagcgcca gggatcagca
    70381 gaacaaacag accaccgtgc ggagccatca gtttcgcacc aatcgccatt gagattgcgc
    70441 cagtcagcgc cccaccaacg atacagcacg gtagcacacg catcggatca cgagcagcaa
    70501 acggaattgc accttccgaa ataaagcaca gaccaagtac cagagcggct ttgccacctt
    70561 cctgctgcgc tttatcgaat ttacgacgcg ccaccattgt tgccagaccc attgccagcg
    70621 gtggcaccat acctgccgcc ataatcgccg ccatcgggcc ataggtttga gtactcagca
    70681 gacccacacc gaatgcgtac gctgctttgt ttaccggacc gcccatgtca gtacacatca
    70741 tgccaccgag gatcgccccc agcagaaccg cattcgcagt ccccatggtc tgcagccagt
    70801 gagtcaaccc atcgagaatg ccagcaaccg gtttaccgat taggtagatc atcgccagac
    70861 cgaccaccag actggaaatt agcggaatga tcaggatcgg tttcagtgcc tccatactct
    70921 gtggcagttt cagttgcgta ctgattaact tcgcaatgta acccgccagg aagcccgcaa
    70981 taataccgcc aatgaagcca gaaccggtgc taaccgccag cataccgcca atcagacccg
    71041 gcgtgagacc cggacgatcg gcaatggaga aggcaatata acctgccagt accggcacca
    71101 tcagcgcaaa ggctgaacca ccaccaatct gcatcagcgc cgctgccaac gtacccggct
    71161 ctttaaacgc ttcgatacca aaagcaaaag aaagcgcgat acacagacca cctgcaacaa
    71221 ccatcggcag catgtaagag acgcccgtca gcaagtgacg gtaagcgcct gcactctctt
    71281 tcttaccttc agtggtcgct gtttgagctt tgcccgctgg ttcatacggc gttgcttcag
    71341 caaccgcttt atccagttcc tgcgcagttt tcttcagcgc cagaccggta gaggtgcgat
    71401 acatcggttt accagcaaat ttcgccagat ccacttcgat atctgccgcc acaatcacca
    71461 gatccgctgc tgcgacttct tcaggtgtga ttgcattacc cgcgccaaca gaaccacggg
    71521 tttcaacttt cacccaccag ccacgttttt tcgcttcggt ttcaatggct tcagccgcca
    71581 taaaggtgtg tgctacacca gtcgggcaag cagtcaccgc aactacgcgt ttcggaccgc
    71641 tagcagcaac cggtgctgtc gcagcgaccg gcgcagtgta aggtttcgca tggcctttgg
    71701 cttcactcag gaacagctca gggtgcgcaa ctgcccggga aatatcgccc agccagacat
    71761 ttttaccgtt cagcgcgctg tcattcggga tgaaatcacc gagaacaatc gccatttctg
    71821 cgtcgttcgg attgtcgatg atttccagtt ttgcttttcg cgccgccgcg cccagcaggg
    71881 tcttcgccat ataggcgcgt gcctgaccga gattagcgtc aataatcagc agcgttttca
    71941 ttatgcctct cctgctgtca gttaaaaggt tgtaagtcga cgcgcgccat cattgcggcc
    72001 aactgcggac gatcggtaat acccacattg ctttgactta ccgccagggc tgcaacagct
    72061 gtcgccagac gcagtgtgtg ttcactggat tcacgcatca gcaagccata aatcaggcca
    72121 ccaaccatag aatcccctgc gccaacggtg cttacgacat cgactgacgg tggtttggcg
    72181 atccattcgc cggaggcatt aacccaaagc gcgccttcgg cacccagtga aataacaaca
    72241 tgcgcaatac cttgttcacg tagcgcatgt gcagcttcaa tcacatcttt catttcaggc
    72301 agtttacggc ctgcccagat ttccagctcg cggcggttag gtttcaccag ccacggtgcc
    72361 gctttcaaac ctgctactaa cgcttcacgg ctactatcaa agataatgca aggacactga
    72421 ctacgcaggc gagtcatcca gtcggtgaac gcttccgggc tgacgcctga cggtaagctt
    72481 ccgctgacac agaccatatc gaactgaccg agccagctca gagaatcagt cacaaagcgt
    72541 tcccagtcgg cgggggtaac ttcaaaaccc gagaagttga agtcggtcac ttcgccgtct
    72601 ttttccgtca gcttaacgtt aattcgggtg cgcccctgta caacctggaa acggttggca
    72661 atgcccagct cgctgaacag ttgctgaaaa ccatcctgat tgtctttacc caggaagccg
    72721 ccaacggtga catcaattcc caggtctttt aatactttgg ccacgttgat gcctttaccc
    72781 gccgcatgca gaccggtggt tttcaccagg ttcacttcgc cgcgttcaat ttccgggcag
    72841 aaaccaacaa ggtcataagc cggattaagg gtgatcgtag caacacgtct gctcattatg
    72901 cgccctcccc aagaccagca gcgatagcgt cgccaatggc tttcagcgcc tgttcagcat
    72961 ctgcaccctg ggcggtaaag cgtaggcgat gacctttctt aacgccaagt gccacaactt
    73021 tcatcagact acgtccgttt gccggtttgc cggtgccatc aaggtttgtc acggtaatat
    73081 cactgttaaa ttgtttaatg gtattgacca gcatggtacc tggacgagca tgcaggccgt
    73141 gttcattgcg caccacaaac tccgcgctta acacgtcgtc ggtcggcgca tcatcgctgg
    73201 tcagcagcgc cagcaacgtt gccgcatccg ctttcagcaa gcggtcagct ttattatcga
    73261 gcaacaaatc agcgagacgc ttaagaaccg cgatgggctg atcgtcattc attgccacac
    73321 tcaccagcat ggctgccgtt tcgccgtcca catcaaaagc atttgccgca cggcttaccg
    73381 caatcgcgct acgcagattg ccttcggcgc tatcgctcag ccagataccc tgtccgaggt
    73441 tcagcggttg ttcattgatg gctttggtga cgaaagtggc gtcaactgcc cccgcttctt
    73501 tcagacgcgc agcgttcagc gcctgaagag tcagcagatc gctggcgacg atatccagcg
    73561 tcagcatttc gttgtcgagc ttcagctgct cactctgctt ttcgcccatc agcaatgcgc
    73621 gaagttcttc tgctgttgtt gctgacttca gttgttcagc aacggaatca tcgctcagta
    73681 cgtgggtcag ctggcgtagc aggcccagat gttcatccga gctggcagca ataccgatcg
    73741 ccacgtacgc tacctggccg tccccccagg tgacgccttc cgggaactga aatacctgaa
    73801 cgccggtttt cagcacctga tcgcgggtgt cggtagtgcc gtgtggaata gcaataccat
    73861 tgccgaggaa cgttgaggtt tgctgttcgc gcgccagcat gccattgacg tagccttcag
    73921 ctacattacc ggcctgcacc agcgccgcag cgacctggcg aatcgcctct tctttgtctc
    73981 cggccttttc gcccggatgg atgtcctgta cagataactg gaacatagtt ctcctctctt
    74041 gctgaattga aacgattcag cttctatgag aaaaaaagcg ccaacctggc ttagggttaa
    74101 agacaagatc gcgctgaaac gtttcaagga agcataatac ttctgtttca gcgcgcaagg
    74161 aaagccgtaa agttatgagc aaaagtttga tgtgctgcac aattttttgt catttttcct
    74221 aattgctgat gggaaaatcc gttatcagca atttcatttc agcagtgtca gcagccaatc
    74281 agcaacagtg aaatggcatt ttgattattc gtggcgcttt tgatttcgct gtctgtttat
    74341 tttctgacaa gcagcgtaaa ctccgcgtct tcctcttcca gtgatcgacc agcatgcata
    74401 actcccccac agtcaccagc gcgaaatcgt ttgacctgac ctcgacggcg tttttaatcg
    74461 ttgcctttct caccggtatt gcgggcgctc tgcaaacccc gacactcagt atttttctta
    74521 ctgatgaagt acatgcccgt ccggcgatgg tgggattctt ctttaccggc agcgctgtca
    74581 ttgggattct ggtgagtcag tttctcgccg ggcgctctga taagcgcggc gatcgcaaat
    74641 cgctgattgt cttttgctgc ctgttaggcg tgctggcctg cacccttttt gcctggaatc
    74701 gcaactactt tgttttgcta ttcgttggcg tctttcttag cagctttggc tcgaccgcta
    74761 acccgcaaat gtttgccctt gcccgtgaac atgctgacaa aaccggacgt gaggcggtga
    74821 tgttcagctc ttttttacgc gctcaggttt cactggcatg ggtcattggc ccaccgctgg
    74881 cttatgcctt agcgatgggt ttcagcttta cggtaatgta tctgagcgca gcggtagcgt
    74941 ttattgtttg cggcgtgatg gtgtggctgt ttttgccgtc gatgcaaaaa gagcttccgc
    75001 tggcgaccgg cacggttgaa gcgccgcgcc gtaaccgtcg cgatacgttg ctgctgtttg
    75061 tcatttgtac attgatgtgg ggctcgaaca gcctgtacat catcaacatg ccgctattta
    75121 ttatcaacga actgcatctt cccgagaaac tggccggtgt gatgatgggg accgccgccg
    75181 ggctggaaat cccgaccatg ttgatcgccg gatattttgc caaacgtctg ggtaagcgct
    75241 tcttaatgcg cgttgctgcc gtgggtggcg tctgttttta cgcaggaatg ctgatggcgc
    75301 attcacctgt cattctgttg ggcttgcagc tgctaaatgc tatttttatt ggcattcttg
    75361 gcggcatcgg gatgctctat tttcaggatt tgatgcccgg tcaggcaggt tcagccacca
    75421 cgctctatac caacacttcg cgcgtgggct ggatcatcgc aggatcagtg gcgggcatcg
    75481 tcgccgagat ctggaattat cacgctgtgt tctggtttgc gatggtgatg attatcgcca
    75541 ctctgttttg cttactgcga attaaagatg tttaaggcgc ggtcaacatt tccagatcga
    75601 taagccagat catggcctgc tgacgcgagt taccgcacat ttcagcgctg gcttgtaagc
    75661 ctgcacagac tttcggtcgc agtggcgagg tgaaaatttt acagcgttgc tgttcatcaa
    75721 gctgaatgca gggagtattg gcgggcttgc catctggcat accgggaata gggctggaaa
    75781 ttgaaggggc agtgcaacac gccccgcaac ccggacggca ttccatacgt tgttctctta
    75841 aggttaacca ccggattgat gagcgcgcac agtaccacct tttttgcacc agcaaaagtg
    75901 cgaataccac ttgcccgaaa ggcccgtcgc gagtactttg tcgcgatatt tttgacattt
    75961 ttgactacag gaatttttcg atgccaagag cgaacgaaat taaaaaaggt atggtactga
    76021 attacaacgg caaactgctg ctggttaagg acattgatat tcagtcgccc actgcccgcg
    76081 gtgccgctac gctgtacaaa atgcgttttt ctgatgtccg taccgggctg aaagtagaag
    76141 agcgtttcaa aggtgatgat atcgttgaca ccgtgacgct gacccgccgt tacgttgact
    76201 tctcctatgt cgatggcaac gaatatgtct ttatggataa agaagactat accccgtata
    76261 ccttcaccaa agatcagatt gaagaagagt tgctgtttat gcctgagggc ggcatgccgg
    76321 acatgcaggt gctgacctgg gacggtcagt tgctggcgct tgagctgccg cagaccgttg
    76381 atctggaaat cgttgaaact gcaccaggta tcaaaggggc atctgccagc gcccgtaaca
    76441 aaccggcgac attgagcact ggtctggtga ttcaggtacc agaatactta agcccgggcg
    76501 aaaaaattcg tatccatatc gaagaacgcc gttatatggg tcgtgctgac taacttcagc
    76561 cgcatgcaga aaagggatag ctcaggctgt ccctttttta atttattata ccaatcttct
    76621 attttgcgct tcctgaacac cttacgcccg caattttcac tcgttgattg atcgccctca
    76681 cacttcatcg cattaacaat ccagaccaat ttcaattgct gtcatataac tttacactgt
    76741 cattgttaat taatcgttac taagacgtga ctcctatgaa tacaatcgcc tccgttacgc
    76801 tcccgcatca tgtacacgct ccacgctacg atcgacagca gttgcaatca cgtatcgttc
    76861 attttggctt tggagccttt caccgcgcac atcaggcatt actgaccgat cgtgtgctga
    76921 atgcccaggg cggcgactgg gggatctgtg aaatcagctt gttcagcggc gatcaactga
    76981 tgagccagct ccgcgcacag aaccatttat ataccgtgct ggagaaaggt gcggacggca
    77041 atcaggcgat aattgtcggt gccgttcacg aatgtctgaa tgcaaaactg gattccttag
    77101 cggcaattat tgagaaattt tgcgagccac aggtggcaat tgtttccctg acaattaccg
    77161 aaaaaggcta ttgtattgac ccggccaccg gtgcactcga caccagcaat ccgcgtatta
    77221 ttcacgatct acaaaaccct gaagaacctc actccgcgcc aggtattctc gtcgaagcac
    77281 tgaaacgccg ccgtgagcgc ggccatacac cgtttaccgt gctctcctgc gacaatattc
    77341 ccgacaatgg ccatgtggtg aaaaacgcgg tgctgggaat ggcagaaaaa cgttcgccag
    77401 aactcgccgg gtggataaaa gaacacgtca gttttccggg aaccatggtc gaccgcattg
    77461 ttccggctgc aacaaacgaa tcactggcgg aaataagcca gcatctgggg gtgaatgatc
    77521 cctgcgcgat tagctgcgaa ccgtttatcc agtgggtggt tgaagataac ttcgtcgctg
    77581 ggcgtcctgc ctgggaagtc gcaggtgtac aaatggtgaa tgatgtcctg ccatgggaag
    77641 agatgaaact gcggatgctt aatggcagcc actcttttct cgcttatctg ggttacctct
    77701 caggattcgc ccatatcagt gattgcatgc aggatcgcgc atttcgccat gccgccagaa
    77761 cattaatgct ggatgagcaa gcgccgacac tgcggattaa agatgtcgat ttaacacaat
    77821 atgcggataa gttaattgca cgttttgcta atccggcact gaaacataag acctggcaaa
    77881 tcgcgatgga tggcagccag aaattaccgc aacgcatgtt ggcaggtatt cgcatacatc
    77941 tggggcgcga aacggactgg tcgttgctgg cattaggcgt tgcaggctgg atgcgttacg
    78001 tcagcggcgt tgatgatgcc ggaaatgcca ttgatgttcg cgatccgctt agcgataaaa
    78061 ttcgtgaact tgttgcaggc agcagcagtg aacaacgcgt aaccgccctg ctttccctgc
    78121 gtgaaatttt cggtgatgat ctgccagata acccgcattt tgtgcaggtc atcgaacaag
    78181 cctggcaaca aatcgcacaa ttcggcgcgc atcaggcgct attaaacacc ctcaaaattt
    78241 aacgatttct gcggttaaag cggatgaagc tcgccttcgt ccgctctccc cttctctttt
    78301 ctgccttttt tagccaggat taacgctcag ttaacttacc agagtattgc ggagccgttg
    78361 tgaccagaac caacctcatc accggttttc tcggcagcgg gaaaactacg tcgattcttc
    78421 atctgttagc ccataaagat cccaacgaaa aatgggcggt actggttaat gaatttgggg
    78481 aagtcggaat tgatggtgct ttgctcgccg atagcggcgc actgctgaaa gagatccccg
    78541 gcggttgcat gtgctgcgtt aatggtttac ccatgcaggt agggttgaat accttactgc
    78601 gtcagggaaa accagaccgc ttgttgatag agccgaccgg gctgggccat ccgaaacaga
    78661 tcctcgatct cttaaccgcg ccagtctatg aaccgtggat agatctgcgc gccacgttgt
    78721 gcattctcga tccacgcctg ctgctggacg aaaaaagcgc cagcaatgaa aacttccgtg
    78781 accagctggc tgccgcagac attattgtcg ccaataaatc cgaccgtgcg acgcccgaaa
    78841 gtgagcaagc gctagagcgc tggtggcagc aaaatggtgg cgatcggcaa ttaattcaca
    78901 gcgagcatgg aaaaattgac ggtcatcttc tggatttgcc gcgtcgcaat ttagccgagt
    78961 tgcccgccag cgccgcgcat tctcatcagc atagcgtgaa aaaagggtta gcagcgttaa
    79021 gcctgccaga gcatcaacgc tggcgtcgca gtctgaacag cggacaaggg catcaggcct
    79081 gtggctggat attcgacgct gatacggtat tcgacaccat tggtattctg gaatgggcgc
    79141 gacttgcacc ggtggaacgc gtcaaaggcg tgctgcgtat tcccgaaggg ctggtgcgaa
    79201 tcaaccgtca gggcgatgac ctgcacattg aaacgcaaaa cgttgcgcca ccggacagtc
    79261 gtattgagct gatttccagc agcgaagctg actggaatgc cttgcagagc gcgctgttga
    79321 agcttcgttt agcgactacc gcgtaaggtt gcctgcgttt ttcagtaaga taattagaga
    79381 aaatatgatt aaaaatttgc cgcaaatagt gttgttgaat attgtcggcc tcgcgctgtt
    79441 tct
//
LOCUS       DAHHTG010000004        77929 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.004, whole genome shotgun sequence.
ACCESSION   DAHHTG010000004 DAHHTG010000000
VERSION     DAHHTG010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 77929)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 77929)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..77929
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.004"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            43..486
                     /gene="cmtB"
                     /locus_tag="NBI74_000282"
     CDS             43..486
                     /gene="cmtB"
                     /locus_tag="NBI74_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IIA"
                     /protein_id="HCD1930663.1"
                     /translation="MRLIDYFPESSISVIHSAKDWQEAIDFSMASLLDKNYISENYIQ
                     SIKDSTISNGPYYILAPGVAMPHARPECGALKTGMSLTLLKQGVYFPGKDEPIKLLIG
                     LSAADANSHIGAIQALSELLCEEEILEQLLTASSEKQLADIISRG"
     gene            514..1902
                     /gene="cmtA"
                     /locus_tag="NBI74_000283"
     CDS             514..1902
                     /gene="cmtA"
                     /locus_tag="NBI74_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICB"
                     /protein_id="HCD1930664.1"
                     /translation="MENKSARAKVQAFGGFLTAMVIPNIGAFIAWGFITALFIPTGWL
                     PNEHFAKIVGPMITYLLPVMIGSTGGHLVGGKRGAVMGGIGTIGVIVGAEIPMFLGSM
                     IMGPLGGLVIKYIDKSLEKRIPAGFEMVINNFSLGIAGMLLCLLGFEVIGPAVLIANT
                     FVKECIEALVHAGYLPLLSVINEPAKVLFLNNAIDQGVYYPLGMQQASVNGKSIFFMV
                     ASNPGPGLGLLLAFTLFGKGMSKRSAPGAMIIHFLGGIHELYFPYVLMKPLTIIAMIA
                     GGMSGTWMFNLLDGGLVAGPSPGSIFAYLALTPKGSFLATIAGVTVGTLVSFAITSLI
                     LKMEKTVETKSEDEFTESANAVKAMKQEGAFSLSRVKRIAFVCDAGMGSSAMGATTFR
                     KRLEKAGLAIEVKHYAIENVPADADIVVTHASLEGRVKRVTDKPLILINNYIGDPKLD
                     TLFNQLTAEHKH"
     gene            1917..3194
                     /locus_tag="NBI74_000284"
     CDS             1917..3194
                     /locus_tag="NBI74_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HCD1930665.1"
                     /translation="MKTKVAAIYGKRDVRLREFELPEITDNELLVSVISDSVCLSTWK
                     AALLGSEHKRVPDDLENHPVITGHECAGVVVEVGKNLTGKYKKGQRFVLQPAMGLPSG
                     YSAGYSYEYFGGNATYMIIPEIAINLGCVLPYHGSYFAAASLAEPMCCIIGAYHANYH
                     TTQYVYEHRMGVKPGGNIALLACAGPMGIGAIDYAINGGIQPARVVVVDIDDKRLAQV
                     QKLLPVELAASKGIELVYMNTKGMSDPVQTLRALTDDAGFDDVFVYAAVPSVVEMADE
                     LLAEDGCLNFFAGPTDKNFKVPFNFYNVHYNSTHIVGTSGGSTDDMKEAIALSATGQL
                     QPSFMVTHIGGLDAVPETVLNLPDIPGGKKLIYNGVTMPLTAIADFAEKGKTDPLFKE
                     LARLVEKTHGIWNEQAEKYLLAQFGVDIGEAAQ"
     gene            3191..4156
                     /gene="yggF"
                     /locus_tag="NBI74_000285"
     CDS             3191..4156
                     /gene="yggF"
                     /locus_tag="NBI74_000285"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HCD1930666.1"
                     /translation="MMSLAWPLFRVTEQAALAAWPQTGCGDKNKIDGLAVTAMRQALN
                     DVAFRGRVVIGEGEIDHAPMLWIGEEVGKGDGPEVDIAVDPIEGTRMVAMGQSNALAV
                     MAFAPRDSLLHAPDMYMKKLVVNRLAAGAIDLSLPLADNLRNVARALGKPLDKLRMVT
                     LDKPRLSAAIEEATQLGVKVFALPDGDVAASVLTCWQDNPYDVMYTIGGAPEGVISAC
                     AVKALGGDMQAELIDFCQAKGDYTENRQIAEQERKRCKAMGVDVNRVYSLDELVRGND
                     ILFSATGVTGGELVNGIQQTANGVRTQTLLIGGADQTCNIIDSLH"
     gene            4178..4687
                     /gene="fumE"
                     /locus_tag="NBI74_000286"
     CDS             4178..4687
                     /gene="fumE"
                     /locus_tag="NBI74_000286"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarase E"
                     /protein_id="HCD1930667.1"
                     /translation="MATLTEDDVLEQLNAQDNLFSFMKTAHSILLQGIRQFLPSLFVD
                     NDEEIVEYAVKPLLAQSGPLDDIDVALRLIYALGKMDKWLYADITHFSQFWHYLNEQD
                     ETPRFADDITWDFISNVNSITRNATLYDALKAMKFADFAVWSEARFSGMVKTALTLAV
                     TTTLKELTP"
     gene            4684..5397
                     /locus_tag="NBI74_000287"
     CDS             4684..5397
                     /locus_tag="NBI74_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside/nucleotide kinase family protein"
                     /protein_id="HCD1930668.1"
                     /translation="MKIELTVNGLKVQAQYSDDEIENVHKPLLRMLAALQTVNPQRRT
                     VVFLCAPPGTGKSTLTTFWEYLAQQDPELPAIQTLPMDGFHHYNSWLDVHQLRPFKGA
                     PETFNVAKLAENLCRVVEGDCTWPQYDRQKHDPVEDVLHVTAPLVIVEGNWLLLDDEK
                     WCQLAQFCDFSIFIKAPASALRERLVGRKLAGGLSLADAEAFYDRTDGPNVRRVLEES
                     LPANLTLMMTATGEYRLMD"
     gene            complement(5369..6046)
                     /locus_tag="NBI74_000288"
     CDS             complement(5369..6046)
                     /locus_tag="NBI74_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/molybdate ABC transporter ATP-binding
                     protein"
                     /protein_id="HCD1930669.1"
                     /translation="MLTLNQISYRWPGAATDCLCDISLQLKQGEWLALTGDNGAGKST
                     LLRVMAGLLTPTAGTVMLQQQAMKNLKNRQRAAKIGVLFQEAENQLFHSTVADEIAFG
                     LILQKCPADEITQRTNAALQCCQLADTASAHPLDLHSAQRRMVAVACLEALSPPLLLL
                     DEPSRDFDENWLSVFESWLEKCRQRGTSVVAISHDAAFTRRHFSRVVRLEDGVIRNVN
                     PSDDIHP"
     gene            complement(6040..6717)
                     /locus_tag="NBI74_000289"
     CDS             complement(6040..6717)
                     /locus_tag="NBI74_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HCD1930670.1"
                     /translation="MVTLEQFRYCPTHSTHPPFCYDFHYVKPGMVAIFGDNGSGKSTL
                     AQLMAGWYPDFLPGEITGTGTLLGTPIGRLPLNEQSATIQLVQQSPYLQLSGCTFSVE
                     EEVAFGPENLCLAEKEIMARIDAALALTECQPLRHRHPATLSGGETQRVVIACAIAMQ
                     PKLLILDEAFSRLTPQAREMLLQRLQHWALERGSLIILFERHHTPFLNHCQQAWQLQN
                     GALQPLC"
     gene            complement(6705..7412)
                     /locus_tag="NBI74_000290"
     CDS             complement(6705..7412)
                     /locus_tag="NBI74_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HCD1930671.1"
                     /translation="MHPFTSLTLWALAACTTLILPTQTILPVYSAATFFCLIALKATR
                     RRAKYVVWLMFSLGAGLWLVHGGWLTEWLSGTPHSPERWAHAITLWLRILAIVSTSQL
                     WMQYVPVQRFIRALFASRLPPGVAYLFAGPLLVVEQLKRQLAIIHEAQCARGVPLDEG
                     WYQRLRAMPALIIPLTHNALNDLAVRGAALDMRAFRINNRRTTLWAPADSTLQCVARY
                     AMILLMLAEFGAWIWLR"
     gene            complement(7413..7991)
                     /locus_tag="NBI74_000291"
     CDS             complement(7413..7991)
                     /locus_tag="NBI74_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HCD1930672.1"
                     /translation="MARRHFSSQALVLIVISIAINMIGGQLASMVKLPIFLDSIGTLI
                     SAVLLGPIIGMLTGLLTNLLWGLLTDPIAAAFAPVAMVIGLVAGWLARAGWFRTLPKV
                     VVSGVIITLAVTVVAVPLRTALFGGVTGSGADLFVAWMHSMGQNLVESVAITVIGANL
                     VDKILTAVIVWLLLRQLPIRTTRHFPAMAAVR"
     gene            complement(8014..8445)
                     /locus_tag="NBI74_000292"
     CDS             complement(8014..8445)
                     /locus_tag="NBI74_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HCD1930673.1"
                     /translation="MMTLPYPYSSSARFYALRLLPGQEVLSQLRAFAQQQQLHAAWIA
                     GCTGSLTDVALRYAGQENTALLSGKFEVIALNGTLEQSGEHLHLCVSDPHGTMLGGHM
                     MPGCTVRTTLELVIGCLEELAFSRQLCALSGYDELHISPVK"
     gene            8817..9836
                     /gene="epd"
                     /locus_tag="NBI74_000293"
     CDS             8817..9836
                     /gene="epd"
                     /locus_tag="NBI74_000293"
                     /EC_number="1.2.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="erythrose-4-phosphate dehydrogenase"
                     /protein_id="HCD1930674.1"
                     /translation="MTVRVAINGFGRIGRNVVRALYESGRRAEITVVAINELADAAGM
                     AHLLKYDTSHGRFAWEVRQERDQLFVGDDAIRVLHERSLQSLPWRELGVDVVLDCTGV
                     YGSREHGEAHIAAGAKKVLFSHPGSNDLDATVVYGVNQDQLRAEHRIVSNASCTTNCI
                     IPVIKLLDDAYGIESGTVTTIHSAMHDQQVIDAYHPDLRRTRAASQSIIPVDTKLAAG
                     ITRFFPQFNDRFEAIAVRVPTINVTAIDLSVTVKKPVKANEVNLLLQKAAQGAFHGIV
                     DYTELPLVSVDFNHDPHSAIVDGTQTRVSGAHLIKTLVWCDNEWGFANRMLDTTLAMA
                     TVAFR"
     gene            9886..11049
                     /gene="pgk"
                     /locus_tag="NBI74_000294"
     CDS             9886..11049
                     /gene="pgk"
                     /locus_tag="NBI74_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HCD1930675.1"
                     /translation="MSVIKMTDLDLAGKRVFIRADLNVPVKDGKVTSDARIRASLPTI
                     ELALKQGAKVMVTSHLGRPTEGEYNEEFSLLPVVNYLKDKLSNPVRLVKDYLDGVDVA
                     EGELVVLENVRFNKGEKKDDETLSKKYAALCDVFVMDAFGTAHRAQASTHGIGKFADV
                     ACAGPLLAAELDALGKALKEPARPMVAIVGGSKVSTKLTVLDSLSKIADQLIVGGGIA
                     NTFIAAQGHDVGKSLYEADLVDEAKRLLTTCNIPVPSDVRVATEFSETAPATLKSVND
                     VKADEQILDIGDASAQELAEILKNAKTILWNGPVGVFEFPNFRKGTEIVANAIADSEA
                     FSIAGGGDTLAAIDLFGIADKISYISTGGGAFLEFVEGKVLPAVAMLEERAKK"
     gene            11263..12342
                     /gene="fbaA"
                     /locus_tag="NBI74_000295"
     CDS             11263..12342
                     /gene="fbaA"
                     /locus_tag="NBI74_000295"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016505616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HCD1930676.1"
                     /translation="MSKIFDFVKPGVITGDDVQKVFQVAKENNFALPAVNCVGTDSIN
                     AVLETAAKVKAPVIVQFSNGGASFIAGKGVKSDVPQGAAILGAISGAHHVHQMAEHYG
                     VPVILHTDHCAKKLLPWIDGLLDAGEKHFAATGKPLFSSHMIDLSEESLQENIEICSK
                     YLERMSKIGMTLEIELGCTGGEEDGVDNSHMDASALYTQPEDVDYAYTELSKISPRFT
                     IAASFGNVHGVYKPGNVVLTPTILRDSQEYVSKKHNLPHNSLNFVFHGGSGSTAQEIK
                     DSVSYGVVKMNIDTDTQWATWEGVLNYYKANEAYLQGQLGNPKGEDQPNKKYYDPRVW
                     LRAGQTSMIARLEKAFQELNAIDVL"
     gene            12533..13393
                     /gene="mscS"
                     /locus_tag="NBI74_000296"
     CDS             12533..13393
                     /gene="mscS"
                     /locus_tag="NBI74_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small-conductance mechanosensitive channel MscS"
                     /protein_id="HCD1930677.1"
                     /translation="MEDLNVVDSINGAGSWLVANQALLLSYAVNIVAALAIIIVGLII
                     ARMISNAVNRLMISRKIDATVADFLSALVRYGIIAFTLIAALGRVGVQTASVIAVLGA
                     AGLAVGLALQGSLSNLAAGVLLVMFRPFRAGEYVDLGGVAGTVLSVQIFSTTMRTADG
                     KIIVIPNGKIIAGNIINFSREPARRNEFIIGVAYDSDIDQVKQILTDIIQSEDRILKD
                     REMTVRLNELGASSINFVVRVWSNSGDLQNVYWDVLERIKREFDAAGISFPYPQMDVN
                     FKRVKEDKAA"
     gene            13532..14167
                     /gene="argO"
                     /locus_tag="NBI74_000297"
     CDS             13532..14167
                     /gene="argO"
                     /locus_tag="NBI74_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine exporter ArgO"
                     /protein_id="HCD1930678.1"
                     /translation="MFSYYFQGLALGAAMILPLGPQNAFVMNQGIRRQYHIMIALLCA
                     ISDLVLICAGIFGGSALLMQSPWLLALVTWGGVAFLLWYGFGAFKTAMSSNIELASAE
                     VLKQGRWKIIATMLAVTWLNPHVYLDTFVVLGSLGGQLDVEPKRWFALGTISASFLWF
                     FGLAILAAWLAPRLRTAKSQRIINLVVGCVMWFIALQLARDGIAHAQALFS"
     gene            14260..15000
                     /locus_tag="NBI74_000298"
     CDS             14260..15000
                     /locus_tag="NBI74_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative stress defense protein"
                     /protein_id="HCD1930679.1"
                     /translation="MKFKVIALAALMGISGMAAQANELPDGPHIVTSGTASVDAVPDI
                     ATLAIEVNVAAKDAATAKKQADERVAQYISFLELNQIAKKDISSANLRTQPDYDYQDG
                     KSILKGYRAVRTVEVTLRQLDKLNSLLDGALKAGLNEIRSVSLGVAQPDAYKDKARKA
                     AIDNAIHQAQELANGFNRKLGPVYSVRYHVSNYQPSPMVRMMKADAAPVSAQETYEQA
                     AIQFDDQVDVVFQLEPVDQQPAKTPAAQ"
     gene            15168..16064
                     /locus_tag="NBI74_000299"
                     /pseudo
     CDS             15168..16064
                     /locus_tag="NBI74_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417396.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            complement(16061..17116)
                     /locus_tag="NBI74_000300"
     CDS             complement(16061..17116)
                     /locus_tag="NBI74_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000073199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
                     /protein_id="HCD1930680.1"
                     /translation="MSNVQEWQQLANKELSRREKTVDSLVQQTAEGIAIKPLYTEADL
                     DNLEVTGTLPGLPPYVRGPRATMYTAQPWTIRQYAGFSTAKESNAFYRRNLATHRGYD
                     SDNPRVAGDVGKAGVAIDTVEDIKVLFDQIPLDKMSVSMTMNGAVLPVLAFYIVAAEE
                     QGVTSDKLTGTIQNDILKEYLCRNTYIYPPKPSMRIIADIIAWCSGNMPRFNTISISG
                     YHMGEAGANCVQQVAFTLADRIEYIKAAISAGLKIDDFAPRLSFFFGIGMDLFMNVAM
                     LRAARYLWSEAVSGFGAQDPYNNVIRTTIDHCAHPMYRDYLHRYLENASGGHIHHDLS
                     HVFDLHRNLIATGSMLG"
     gene            complement(17319..18212)
                     /gene="argP"
                     /locus_tag="NBI74_000301"
     CDS             complement(17319..18212)
                     /gene="argP"
                     /locus_tag="NBI74_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator ArgP"
                     /protein_id="HCD1930681.1"
                     /translation="MKRPDYRTLQALDAVIRERGFERAAQKLCITQSAVSQRIKQLEN
                     MFGQPLLVRTVPPRPTEQGQKLLALLRQVELLEEEWLGDEQTGSTPLLLSLAVNADSL
                     ATWLLPALAPVLADSPIRLNLQVEDETRTQERLRRGEVVGAVSIQHQALPSCLVDKLG
                     ALDYLFVSSKPFAEKYFPNGVTRSALLKAPVVAFDHLDDMHQAFLQQNFDLPPGSVPC
                     HIVNSSEAFVQLARQGTTCCMIPHLQIEKELASGELIDLTPGLFQRRMLYWHRFAPES
                     RMMRKVTDALLDYGHKVLRQD"
     gene            18353..18583
                     /locus_tag="NBI74_000302"
     CDS             18353..18583
                     /locus_tag="NBI74_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001328908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1930682.1"
                     /translation="MHFAQRVRALVVLNGVALLPQFACKQGLANGELVRLFAPWSGIP
                     RPLYALFAGRKGMPAIARYFMDELTTRLANGV"
     gene            18639..19298
                     /gene="rpiA"
                     /locus_tag="NBI74_000303"
     CDS             18639..19298
                     /gene="rpiA"
                     /locus_tag="NBI74_000303"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HCD1930683.1"
                     /translation="MTQDELKKAVGWAALQYVQPGTIVGVGTGSTAAHFIDALGTMKG
                     QIEGAVSSSDASTEKLKSLGIHVFDLNEVDSLGIYVDGADEINGHMQMIKGGGAALTR
                     EKIIASVAEKFICIADASKQVDILGKFPLPVEVIPMARSAVARQLVKLGGRPEYRQGV
                     VTDNGNVILDVHGMEILDPIAMENAINAIPGVVTVGLFANRGADVALIGTPDGVKTIV
                     K"
     gene            19554..20786
                     /gene="serA"
                     /locus_tag="NBI74_000304"
     CDS             19554..20786
                     /gene="serA"
                     /locus_tag="NBI74_000304"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HCD1930684.1"
                     /translation="MAKVSLEKDKIKFLLVEGVHQKALESLRAAGYTNIEFHKGALDD
                     EQLKESIRDAHFIGLRSRTHLTEDVINAAEKLVAIGCFCIGTNQVDLDAAAKRGIPVF
                     NAPFSNTRSVAELVIGELLLLLRGVPEANAKAHRGVWNKLAAGSFEARGKKLGIIGYG
                     HIGTQLGILAESLGMYVYFYDIENKLPLGNATQVQHLSDLLNMSDVVSLHVPENPSTK
                     NMMGAKEISLMKPGSLLINASRGTVVDIPALCDALASKHLAGAAIDVFPTEPATNSDP
                     FTSPLCEFDNVLLTPHIGGSTQEAQENIGLEVAGKLIKYSDNGSTLSAVNFPEVSLPL
                     HGGRRLMHIHENRPGVLTALNKIFAEQGVNIAAQYLQTSAQMGYVVIDIEADEDVAEK
                     ALQAMKAIPGTIRARLLY"
     gene            21015..21074
                     /gene="ibsC"
                     /locus_tag="NBI74_000305"
     CDS             21015..21074
                     /gene="ibsC"
                     /locus_tag="NBI74_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsC"
                     /protein_id="HCD1930685.1"
                     /translation="MMRLVIILIVLLLISFSAY"
     gene            complement(21175..21777)
                     /locus_tag="NBI74_000306"
     CDS             complement(21175..21777)
                     /locus_tag="NBI74_000306"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HCD1930686.1"
                     /translation="MTQLPELPLTLSRQEIRKMIRQRRRALTPEQQQEMGQQAATRMM
                     TYPPVVMAHTVAVFLSFDGELDTQPLIEQLWRAGKRVYLPVLHPFSAGNLLFLNYHPQ
                     SELVMNKLKIHEPKLDVRDVLPLSRLDVLITPLVAFDEYGQRLGMGGGFYDRTLQNWQ
                     HYKMQPVGYAHDCQLVEKLPVEEWDIPLPAVVTPSKVWEW"
     gene            complement(21798..21981)
                     /gene="ssrS"
                     /locus_tag="NBI74_000307"
     ncRNA           complement(21798..21981)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="NBI74_000307"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(22023..22352)
                     /gene="zapA"
                     /locus_tag="NBI74_000308"
     CDS             complement(22023..22352)
                     /gene="zapA"
                     /locus_tag="NBI74_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HCD1930687.1"
                     /translation="MSAQPVDIQIFGRSLRVNCPPDQRDALNQAADDLNQRLQDLKER
                     TRVTNTEQLVFIAALNISYELAQEKAKTRDYAASMEQRIRMLQQTIEQALLEQGRITE
                     KTNQNFE"
     gene            22520..23098
                     /gene="ygfB"
                     /locus_tag="NBI74_000309"
     CDS             22520..23098
                     /gene="ygfB"
                     /locus_tag="NBI74_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YgfB"
                     /protein_id="HCD1930688.1"
                     /translation="MSIQNEMPGYNEMNQYLNQQGTGLTPAEMHGLISGMICGGNDDS
                     SWLPLLHDLTNEGMAFGHELAQALRKMHSATSDALQDDGFLFQLYLPDGDDVSVFDRA
                     DALAGWVNHFLLGLGVTQPKLDKVTGETGEAIDDLRNIAQLGYDEDEDQEELEMSLEE
                     IIEYVRVAALLCHDTFTHPQPTAPEVQKPTLH"
     gene            23124..24449
                     /gene="pepP"
                     /locus_tag="NBI74_000310"
     CDS             23124..24449
                     /gene="pepP"
                     /locus_tag="NBI74_000310"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro aminopeptidase"
                     /protein_id="HCD1930689.1"
                     /translation="MSDISRQEFQRRRQALVEQMQPGSAALIFAAPEVTRSADSEYPY
                     RQNSDFWYFTGFNEPEAVLVLIKSDDTHNHSVLFNRVRDLTAEIWFGRRLGQDAAPEK
                     LGVDRALAFSEINQQLYQLLNGLDVVYHAQGEYAYADEIVNSALEKLRKGSRQNLTAP
                     ATMIDWRPVVHEMRLFKSPEEIAVLRRAGEITALAHTRAMEKCRPGMFEYHLEGEIHH
                     EFNRHGARYPSYNTIVGSGENGCILHYTENECELRDGDLVLIDAGCEYKGYAGDITRT
                     FPVNGKFTQAQREIYDIVLESLETSLRLYRPGTSIQEVTGEVVRIMVSGLVKLGILKG
                     DVDELIAQNAHRPFFMHGLSHWLGLDVHDVGVYGQDRSRILEPGMVLTVEPGLYIAPD
                     AEVPEQYRGIGIRIEDDIVITETGNENLTASVVKKPEEIEALMAAARKQ"
     gene            24446..25624
                     /gene="ubiH"
                     /locus_tag="NBI74_000311"
                     /gene_synonym="visB"
     CDS             24446..25624
                     /gene="ubiH"
                     /locus_tag="NBI74_000311"
                     /gene_synonym="visB"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-6-methoxyphenyl hydroxylase"
                     /protein_id="HCD1930690.1"
                     /translation="MSVIIVGGGMAGATLALAISRLSHGALPVHLIEATAPESHAHPG
                     FDGRAIALAAGTCQQLARIGVWQSLADCATAITTVHVSDRGHAGFVTLAAEDYQLAAL
                     GQVVELHNVGQRLFALLRKAPGVTLHCPDRVANVARTQSHVEVTLESGETLTGRVLVA
                     ADGTHSALATACGVDWQQEPYEQLAVIANVATSVAHEGRAFERFTQYGPLAMLPMSDG
                     RCSLVWCHPLERREEVLSWSDEKFCRELQSAFGWRLGQITHAGKRSAYPLALTRAAKP
                     ITHRTVLVGNAAQTLHPIAGQGFNLGMRDVMSLAETLTQAQERREDMGDYGVLCRYQQ
                     RRQRDREATIGVTDSLVHLFANRWTPLVVGRNIGLMTMELFTPARDVLAQRTLGWVAR
                     "
     gene            25647..26849
                     /gene="ubiI"
                     /locus_tag="NBI74_000312"
                     /gene_synonym="visC"
     CDS             25647..26849
                     /gene="ubiI"
                     /locus_tag="NBI74_000312"
                     /gene_synonym="visC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent 2-octaprenylphenol hydroxylase"
                     /protein_id="HCD1930691.1"
                     /translation="MQSVDVAIVGGGMVGLAVACGLQGIGLRVAVLEQRVPEPLAADA
                     PPQLRVSAINAASEKLLTRLGVWQDILSRRACCYHGMEVWDKDSFGHISFDDQSMGYS
                     HLGHIVENSVIHYALWNKALQSSDITLLAPAELQQVAWGENETFLTLKDGSMLTARLV
                     IGADGANSWLRNKADIPLTFWDYQHHALVATIRTEEPHDAVARQVFHGEGILAFLPLS
                     DPHLCSIVWSLSPEEAQRMQQAGEDEFNRALNIAFDNRLGLCKVESERLVFPLTGRYA
                     RQFAAHRLALVGDAAHTIHPLAGQGVNLGFMDAAELIAELKRLHRQGKDIGQYIYLRR
                     YERSRKHSAALMLAGMQGFRDLFSGANPAKKLLRDIGLKLADTLPGVKPQLIRQAMGL
                     NDLPEWLR"
     gene            complement(26923..27006)
                     /locus_tag="NBI74_000313"
     CDS             complement(26923..27006)
                     /locus_tag="NBI74_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1930692.1"
                     /translation="MSKNTKSKIRGIRKYNAKSQVKLVYFK"
     gene            27297..28391
                     /gene="gcvT"
                     /locus_tag="NBI74_000314"
     CDS             27297..28391
                     /gene="gcvT"
                     /locus_tag="NBI74_000314"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HCD1930693.1"
                     /translation="MAQQTPLYEQHTLCGARMVDFHGWMMPLHYGSQIDEHHAVRTDA
                     GMFDVSHMTIVDLRGSRTREFLRYLLANDVAKLTKSGKALYSGMLNASGGVIDDLIVY
                     YFTEDFFRLVVNSATREKDLSWITQHAEPFGIEITVRDDLSMIAVQGPNAQAKAATLF
                     NDAQRQAVEGMKPFFGVQAGDLFIATTGYTGEAGYEIALPNEKAADFWRALVEAGVKP
                     CGLGARDTLRLEAGMNLYSQEMDETISPLAANMGWTIAWEPADRDFIGREALEAQREH
                     GTEKLVGLVMTEKGVLRNELPVRFTDAQGNQHEGIITSGTFSPTLGYSIALARVPEGI
                     GETAIVQIRNREMPVKVTKPVFVRNGKAVA"
     gene            28415..28804
                     /gene="gcvH"
                     /locus_tag="NBI74_000315"
     CDS             28415..28804
                     /gene="gcvH"
                     /locus_tag="NBI74_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HCD1930694.1"
                     /translation="MSNVPAELKYSKEHEWLRKEADGTYTVGITEHAQELLGDMVFVD
                     LPEVGATVSAGDDCAVAESVKAASDIYAPVSGEIVAVNDALSDSPELVNSEPYAGGWI
                     FKIKASDESELESLLDATAYEALLEDE"
     gene            28923..31796
                     /gene="gcvP"
                     /locus_tag="NBI74_000316"
     CDS             28923..31796
                     /gene="gcvP"
                     /locus_tag="NBI74_000316"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HCD1930695.1"
                     /translation="MTQTLSQLENSGAFIERHIGPDAAQQQEMLNAVGAQSLNALTGQ
                     IVPKDIQLATPPQVGAPATEYAALAELKAIASRNKRFTSYIGMGYTAVQLPPVILRNM
                     LENPGWYTAYTPYQPEVSQGRLEALLNFQQVTLDLTGLDMASASLLDEATAAAEAMAM
                     AKRVSKLKNANRFFVASDVHPQTLDVVRTRAETFGFEVIVDDAQKVLDHQDVFGALLQ
                     QVGTTGEIHDYTALISELKSRKIVVSVAADIMALVLLTAPGKQGADIVFGSAQRFGVP
                     MGYGGPHAAFFAAKDEYKRSMPGRIIGVSKDAAGNTALRMAMQTREQHIRREKANSNI
                     CTSQVLLANIASLYAVYHGPVGLKRIANRIHRLTDILAAGLQQKGLKLRHAHYFDTLC
                     VEVADKAGVLARAEAAEINLRSDILNAVGITLDETTTRENVMQLFSVLLGDNHGLDID
                     TLDKDVAHDSRSIQAAMLRDDEILTHPVFNRYHSETEMMRYMHSLERKDLALNQAMIP
                     LGSCTMKLNAAAEMIPITWPEFAELHPFCPPEQAEGYQQMIAQLAEWLVKLTGYDAVC
                     MQPNSGAQGEYAGLLAIRHYHESRNEGHRDICLIPASAHGTNPASAHMAGMQVVVVAC
                     DKNGNIDLTDLRAKAEQAGDNLSCIMVTYPSTHGVYEETIREVCEVVHQFGGQVYLDG
                     ANMNAQVGITSPGFIGADVSHLNLHKTFCIPHGGGGPGMGPIGVKAHLAPFVPGHSVV
                     QIEGMLTRQGAVSAAPFGSASILPISWMYIRMMGAEGLKKASQVAILNANYIASRLQD
                     AFPVLYTGRDGRVAHECILDIRPLKEETGISELDIAKRLIDYGFHAPTMSFPVAGTLM
                     VEPTESESKVELDRFIDAMLAIRAEIDQVKAGVWPLEDNPLVNAPHIQSELVAEWAHP
                     YSREVAVFPAGVADKYWPTVKRLDDVYGDRNLFCSCVPISEYQ"
     gene            complement(31962..33395)
                     /gene="bglA"
                     /locus_tag="NBI74_000317"
     CDS             complement(31962..33395)
                     /gene="bglA"
                     /locus_tag="NBI74_000317"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase BglA"
                     /protein_id="HCD1930696.1"
                     /translation="MKKLTLPKDFLWGGAVAAHQVEGGWNKGGKGPSICDVLTGGAHG
                     VPREITKEVVPGKYYPNHEAVDFYGHYKEDIKLFAEMGFKCFRTSIAWTRIFPKGDEA
                     QPNEEGLKFYDDMIDELLKYNIEPVITLSHFEMPLHLVQQYGSWTNRKVVDFFVRFAE
                     VVFERYKHKVKYWMTFNEINNQRNWRAPLFGYCCSGVVYTEHENPEETMYQVLHHQFV
                     ASALAVKAAHRINPEMKVGCMLAMVPLYPYSCNPDDVMFAQKSMRERYVFTDVQLRGY
                     YPSYVLNEWERRGFNIKMEDGDLDVLREGTCDYLGFSYYMTNAVKAEGGTGDAISGFE
                     GSVPNPYVKASDWGWQIDPVGLRYALCELYERYQKPLFIVENGFGAYDKVEDDGSIND
                     DYRIDYLRAHIEEMKKAVTYDGVDLMGYTPWGCIDCVSFTTGQYSKRYGFIYVNKHDD
                     GTGDMSRSRKKSFNWYKEVIASNGENL"
     gene            33440..33751
                     /locus_tag="NBI74_000318"
     CDS             33440..33751
                     /locus_tag="NBI74_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HCD1930697.1"
                     /translation="MQPNDITFFQRFQDDILAGRKTITIRDESESHFKTGDVLRVGRF
                     EDDGYFCTIEVTATSTVTLDTLTEKHAEQENMTLTELKKVIADIYPDQTQFYVIEFKC
                     L"
     gene            33915..34574
                     /locus_tag="NBI74_000319"
     CDS             33915..34574
                     /locus_tag="NBI74_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000250275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HCD1930698.1"
                     /translation="MVQKPLIKQGYSLAEEIANSVSHGIGLVFGIVGLVLLLVQAVDL
                     NASATAITSYSLYGGSMILLFLASTLYHAIPHQRAKMWLKKFDHCAIYLLIAGTYTPF
                     LLVGLDSPLARGLMIVIWSLALLGILFKLTIAHRFKILSLVTYLAMGWLSLVVIYEMA
                     VKLAAGSVTLLAVGGVVYSLGVIFYVCKRIPYNHAIWHGFVLGGSVCHFLAIYLYIGQ
                     V"
     gene            complement(34651..35631)
                     /gene="ygfZ"
                     /locus_tag="NBI74_000320"
     CDS             complement(34651..35631)
                     /gene="ygfZ"
                     /locus_tag="NBI74_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-modifying protein YgfZ"
                     /protein_id="HCD1930699.1"
                     /translation="MAFTPFPPRQPTASARLPLTLMTLDDWALATITGADSEKYMQGQ
                     VTADVSQMTEDQHLLAAHCDAKGKMWSNLRLFRDGDGFAWIERRSVREPQLTELKKYA
                     VFSKVTIAPDDERVLLGVAGFQARAALANLFSELPSREKQVVKEGATTLLWFEHPAER
                     FLIVTDEATANMLTDKLRGEAELNNSQQWLALNIEAGFPVIDAANSGQFIPQATNLQA
                     LGGISFKKGCYTGQEMVARAKFRGANKRALWLLTGSASRLPEAGEDLELKMGENWRRT
                     GTVLAAVKLEDGQVVVQVVMNNDMEPDSIFRVRDDANTLRIEPLPYSLEE"
     gene            35874..36140
                     /gene="sdhE"
                     /locus_tag="NBI74_000321"
     CDS             35874..36140
                     /gene="sdhE"
                     /locus_tag="NBI74_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD assembly factor SdhE"
                     /protein_id="HCD1930700.1"
                     /translation="MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRTFI
                     RLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRNRERGPVAI"
     gene            36121..36528
                     /locus_tag="NBI74_000322"
     CDS             36121..36528
                     /locus_tag="NBI74_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgfX"
                     /protein_id="HCD1930701.1"
                     /translation="MVLWQSDLRVSWRAQWLSLLIHGLVAAVILLIPWPLSYTPLWMV
                     LLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQEWSIVKAPWMIKSGMMLRLRSD
                     SGKRQHLWLAADSMDEAEWRDLRRILLQQETQR"
     gene            complement(36568..37089)
                     /gene="fldB"
                     /locus_tag="NBI74_000323"
     CDS             complement(36568..37089)
                     /gene="fldB"
                     /locus_tag="NBI74_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldB"
                     /protein_id="HCD1930702.1"
                     /translation="MNMGLFYGSSTCYTEMAAEKIRDIIGPELVTLHNLKDDSPKLME
                     QYDVLILGIPTWDFGEIQEDWEAVWDQLDDLNLEGKIVALYGLGDQLGYGEWFLDALG
                     MLHDKLSTKGVKFVGYWPTEGYEFTSPKPVIADGQLFVGLALDETNQYDLSDERIQSW
                     CEQILNEMAEHYA"
     gene            37201..38097
                     /gene="xerD"
                     /locus_tag="NBI74_000324"
     CDS             37201..38097
                     /gene="xerD"
                     /locus_tag="NBI74_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HCD1930703.1"
                     /translation="MKQDLARIEQFLDALWLEKNLAENTLNAYRRDLSMMVEWLHHRG
                     LTLATAQSDDLQALLAERLEGGYKATSSARLLSAVRRLFQYLYREKFREDDPSAHLAS
                     PKLPQRLPKDLSEAQVERLLQAPLIDQPLELRDKAMLEVLYATGLRVSELVGLTMSDI
                     SLRQGVVRVIGKGNKERLVPLGEEAVYWLETYLEHGRPWLLNGVSIDVLFPSQRAQQM
                     TRQTFWHRIKHYAVLAGIDSEKLSPHVLRHAFATHLLNHGADLRVVQMLLGHSDLSTT
                     QIYTHVATERLRQLHQQHHPRA"
     gene            38122..38832
                     /gene="dsbC"
                     /locus_tag="NBI74_000325"
     CDS             38122..38832
                     /gene="dsbC"
                     /locus_tag="NBI74_000325"
                     /EC_number="5.3.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional protein-disulfide
                     isomerase/oxidoreductase DsbC"
                     /protein_id="HCD1930704.1"
                     /translation="MKKGFMLFTLLAAFSGFVQADDAAIQQTLAKMGIKSSDIQPAPV
                     AGMKTVLTNSGVLYITDDGKHIIQGPMYDVSGTAPVNVTNKMLLKQLNALEKEMIVYK
                     APQEKHVITVFTDITCGYCHKLHEQMADYNALGITVRYLAFPRQGLDSDAEKEMKAIW
                     CAKDKNKAFDDVMAGKSVAPASCDVDIADHYALGVQLGVSGTPAVVLSNGTLVPGYQP
                     PKDMKEFLDEHQKMTSGK"
     gene            38838..40571
                     /gene="recJ"
                     /locus_tag="NBI74_000326"
     CDS             38838..40571
                     /gene="recJ"
                     /locus_tag="NBI74_000326"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HCD1930705.1"
                     /translation="MKQQIQLRRREVDETADLPAELPPLLRRLYASRGVRSAQELERS
                     VKGMLPWQQLSGVEKAVEILYNAFREGTRIIVVGDFDADGATSTALSVLAMRSLGCSN
                     IDYLVPNRFEDGYGLSPEVVDQAHARGAQLIVTVDNGISSHAGVEHARSLGIPVIVTD
                     HHLPGDTLPAAEAIINPNLRDCNFPSKSLAGVGVAFYLMLALRTFLRDQGWFDERGIA
                     IPNLAELLDLVALGTVADVVPLDANNRILTWQGMSRIRAGKCRPGIKALLEVANRDPQ
                     KLAASDLGFALGPRLNAAGRLDDMSVGVALLLCDNIGEARVLANELDALNQTRKEIEQ
                     GMQVEALTLCEKLERSRDTLPGGLAMYHPEWHQGVVGILASRIKERFHRPVIAFAPAG
                     DGTLKGSGRSIQGLHMRDALERLDTLYPGMMLKFGGHAMAAGLSLEEEKFELFQQRFG
                     ELVTEWLDPSLLQGEVVSDGPLSPAEMTMEVAQLLRDAGPWGQMFPEPLFDGHFRLLQ
                     QRLVGERHLKVMVEPVGGGPLLDGIAFNVDTALWPDNGVREVQLAYKLDINEFRGNRS
                     LQIIIDNIWPI"
     gene            40662..41760
                     /gene="prfB"
                     /locus_tag="NBI74_000327"
     CDS             join(40662..40736,40738..41760)
                     /gene="prfB"
                     /locus_tag="NBI74_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311790.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HCD1930706.1"
                     /translation="MFEINPVNNRIQDLTERSDVLRGYLDYDAKKERLEEVNAELEQP
                     DVWNEPERAQALGKERSSLEAVVDTLDQMKQGLEDVSGLLELAVEADDEETFNEAVAE
                     LDALEEKLAQLEFRRMFSGEYDSADCYLDIQAGSGGTEAQDWASMLERMYLRWAESRG
                     FKTEIIEESEGEVAGIKSVTIKISGDYAYGWLRTETGVHRLVRKSPFDSGGRRHTSFS
                     SAFVYPEVDDDIDIEINPADLRIDVYRASGAGGQHVNRTESAVRITHIPTGIVTQCQN
                     DRSQHKNKDQAMKQMKAKLYELEMQKKNAEKQAMEDNKSDIGWGSQIRSYVLDDSRIK
                     DLRTGVETRNTQAVLDGSLDQFIEASLKAGL"
     gene            41770..43287
                     /gene="lysS"
                     /locus_tag="NBI74_000328"
     CDS             41770..43287
                     /gene="lysS"
                     /locus_tag="NBI74_000328"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HCD1930707.1"
                     /translation="MSEQHAQGADAVVDLNNELKTRREKLANLREQGIAFPNDFRRDH
                     TSDQLHAEFDGKENEELEALNIEVAVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARD
                     DLPEGVYNEQFKKWDLGDILGAKGKLFKTKTGELSIHCTELRLLTKALRPLPDKFHGL
                     QDQEARYRQRYLDLISNDESRNTFKVRSQILSGIRQFMVNRGFMEVETPMMQVIPGGA
                     AARPFITHHNALDLDMYLRIAPELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFT
                     MMELYMAYADYKDLIELTESLFRTLAQDILGKTEVTYGDVTLDFGKPFEKLTMREAIK
                     KYRPETDMADLDNFDSAKAIAESIGIHVEKSWGLGRIVTEIFEEVAEAHLIQPTFITE
                     YPAEVSPLARRNDVNPEITDRFEFFIGGREIGNGFSELNDAEDQAQRFLDQVAAKDAG
                     DDEAMFYDEDYVTALEHGLPPTAGLGIGIDRMVMLFTNSHTIRDVILFPAMRPVK"
     gene            complement(43330..43878)
                     /gene="idi"
                     /locus_tag="NBI74_000329"
     CDS             complement(43330..43878)
                     /gene="idi"
                     /locus_tag="NBI74_000329"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HCD1930708.1"
                     /translation="MQTEHVILLNAQGVPTGTLEKYAAHTADTLLHLAFSSWLFNAKG
                     QLLVTRRALSKKAWPGVWTNSVCGHPQLGESNEEAVIRRCRYELGVEITPPESIYPDF
                     RYRATDPNGIVENEVCPVFAARTTSALQINDDEVMDYQWCDLAAVLRGIDATPWAFSP
                     WMVMQATNREARKRLSAFTQLK"
     gene            43933..44004
                     /locus_tag="NBI74_000330"
     CDS             43933..44004
                     /locus_tag="NBI74_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1930709.1"
                     /translation="MINQVSVYRQPPVLSGCRQVKTI"
     gene            complement(44001..44126)
                     /locus_tag="NBI74_000331"
     CDS             complement(44001..44126)
                     /locus_tag="NBI74_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1930710.1"
                     /translation="MNLLMRAIFSLLLLFTLSIPVISDCVAMAIESRFKYMMLLF"
     gene            complement(44128..45576)
                     /gene="uacT"
                     /locus_tag="NBI74_000332"
     CDS             complement(44128..45576)
                     /gene="uacT"
                     /locus_tag="NBI74_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urate/proton symporter UacT"
                     /protein_id="HCD1930711.1"
                     /translation="MSAIDSQLPSSSGQDRPTDEVDRILSPGKLIILGLQHVLVMYAG
                     AVAVPLMIGDRLGLSKEAIAMLISSDLFCCGIVTLLQCIGIGRFMGIRLPVIMSVTFA
                     AVTPMIAIGMNPDIGLLGIFGATIAAGFITTLLAPLIGRLMPLFPPLVTGVVITSIGL
                     SIIQVGIDWAAGGKGNPQYGNPVYLGISFAVLIFILLITRYAKGFMSNVAVLLGIVFG
                     FLLSWMMNEVNLSGLHDASWFAIVTPMSFGMPIFDPVSILTMTAVLIIVFIESMGMFL
                     ALGEIVGRKLSSQDIIRGLRVDGVGTMIGGTFNSFPHTSFSQNVGLVSVTRVHSRWVC
                     ISSGIILILFGMVPKMAVLVASIPQFVLGGAGLVMFGMVLATGIRILSRCNYTTNRYN
                     LYIVAISLGVGMTPTLSHDFFSKLPAVLQPLLHSGIMLATLSAVVLNVFFNGYQHHAD
                     LVKESVSDKDLKVRTVRMWLLMRKLKKNEHGE"
     gene            46012..47931
                     /gene="ygfT"
                     /locus_tag="NBI74_000333"
     CDS             46012..47931
                     /gene="ygfT"
                     /locus_tag="NBI74_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent uric acid utilization protein
                     YgfT"
                     /protein_id="HCD1930712.1"
                     /translation="MNKFIAAEAAECIGCHACEIACAVAHNQENWPLSHSDFRPRIHV
                     VGKGQAANPVACHHCNNAPCVTACPVNALTFQSDSVQLDEQKCIGCKRCAIACPFGVV
                     EMVDTIAQKCDLCNQRSSGTQACIDVCPTQALRLMDDKGLQQIKVARQRKTAAGKASS
                     DAQPSRSAALLPVNSRKGADKISASERKTHFGEIYCGLDPQQATYESDRCVYCAEKAN
                     CNWHCPLHNAIPDYIRLVQEGKIIEAAELCHQTSSLPEICGRVCPQDRLCEGACTLKD
                     HSGAVSIGNLERYITDTALAMGWRPDVSKVVPRSEKVAVIGAGPAGLGCADILARAGV
                     QVDVFDRHPEIGGMLTFGIPPFKLDKTVLSQRREIFTAMGIDFHLNCEIGRDISFNEL
                     TAEYDAVFLGVGTYGMMRADLPHEDAPGVIQALPFLTAHTRQLMGLPESAEYPLTDVE
                     GKRVVVLGGGDTTMDCLRTSIRLNAASVTCAYRRDEVSMPGSRKEVVNAREEGVEFQF
                     NVQPQYIACDEDGRLTAVGLIRTAMGEPGPDGRRRPRPVAGSEFELPADVLIMAFGFQ
                     AHTMPWLQGSGIKLDKWGLIQTGDVGYLPTQTHLKKVFAGGDAVHGADLVVTAMAAGR
                     QAARDMLTLFDTKAS"
     gene            47931..48419
                     /locus_tag="NBI74_000334"
     CDS             47931..48419
                     /locus_tag="NBI74_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417362.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HCD1930713.1"
                     /translation="MKSLIIVNPADCIGCRTCEVACVVAHPSEQELNADIFLPRLKVQ
                     RLDSISAPVMCHQCENAPCVGACPVGALTMGEQVVQANSARCIGCQSCVSACPFGMIT
                     IQSLPGDTRQQIVKCDLCEQREEGPACVESCPTQALQLLTERELRRVRQQRIVASSEN
                     PL"
     gene            complement(48455..49822)
                     /gene="ghxQ"
                     /locus_tag="NBI74_000335"
                     /gene_synonym="ygfQ"
     CDS             complement(48455..49822)
                     /gene="ghxQ"
                     /locus_tag="NBI74_000335"
                     /gene_synonym="ygfQ"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanine/hypoxanthine transporter GhxQ"
                     /protein_id="HCD1930714.1"
                     /translation="MSGDILQTPDAPKPQGALDNYFKITARGSTVRQEVLAGLTTFLA
                     MVYSVIVVPGMLGKAGFPPAAVFVATCLVAGFGSLLMGLWANLPMAIGCAISLTAFTA
                     FSLVLGQQISVPVALGAVFLMGVIFTAISVTGVRTWILRNLPMGIAHGTGIGIGLFLL
                     LIAANGVGMVIKNPIEGLPVALGAFTSFPVMMSLLGLAVIFGLEKCRVPGGILLVIIA
                     ISIIGLIFDPAVKYHGLVAMPSLTGEDGKSLIFSLDIMGALQPTVLPSVLALVMTAVF
                     DATGTIRAVAGQANLLDKDNQIINGGKALTSDSVSSIFSGLVGAAPAAVYIESAAGTA
                     AGGKTGLTATVVGVLFLMILFLSPLSFLIPGYATAPALMYVGLLMLSNVSKLDFNDFI
                     DAMAGLVCAVFIVLTCNIVTGIMLGFVTLVVGRVFAREWQKLNIGTVIITAALVAFYA
                     GGWAI"
     gene            complement(49858..51174)
                     /gene="guaD"
                     /locus_tag="NBI74_000336"
     CDS             complement(49858..51174)
                     /gene="guaD"
                     /locus_tag="NBI74_000336"
                     /EC_number="3.5.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanine deaminase"
                     /protein_id="HCD1930715.1"
                     /translation="MSGEHTLKAVRGSFIDVTRTVDNPEEIASALRFIEDGLLLIKQG
                     KVEWFGEWEDGKHQIPDTIRVRDYRGKLIVPGFIDTHIHYPQSEMVGAYGEQLLEWLN
                     KHTFPTERRYEDLEYAREMSAFFIKQLLRNGTTTALVFGTVHPQSVDALFEAASHINM
                     RMIAGKVMMDRNAPDYLLDTAESSYHQSKELIERWHKNGRLLYAITPRFAPTSSPEQM
                     AMAQRLKEEYPDTWVHTHLCENKDEIAWVKSLYPDHDGYLDVYHQYGLTGKNCVFAHC
                     VHLEEKEWDRLSETKSSIAFCPTSNLYLGSGLFNLKKAWQKKVKVGMGTDIGAGTTFN
                     MLQTLNEAYKVLQLQGYRLSAYEAFYLATLGGAKSLGLDDLIGNFLPGKEADFVVMEP
                     TATPLQQLRYDNSVSLVDKLFVMMTLGDDRSIYRTYVDGRLVYERN"
     gene            complement(51192..52592)
                     /gene="xanQ"
                     /locus_tag="NBI74_000337"
     CDS             complement(51192..52592)
                     /gene="xanQ"
                     /locus_tag="NBI74_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417358.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine/proton symporter XanQ"
                     /protein_id="HCD1930716.1"
                     /translation="MSDINHAGSDLIFELEDRPPFHQALVGAITHLLAIFVPMVTPAL
                     IVGAALQLSAETTAYLVSMAMIASGIGTWLQVNRYGIVGSGLLSIQSVNFSFVTVMIA
                     LGSSMKSDGFHEELIMSSLLGVSFVGAFLVVGSSFILPYLRRVITPTVSGIVVLMIGL
                     SLIKVGIIDFGGGFAAKSSGTFGNYEHLGVGLLVLIVVIGFNCCRSPLLRMGGIAIGL
                     CVGYIASLCLGMVDFSSMRNLPLITIPHPFKYGFSFSFHQFLVVGTIYLLSVLEAVGD
                     ITATAMVSRRPIQGEEYQSRLKGGVLADGLVSVIASAVGSLPLTTFAQNNGVIQMTGV
                     ASRYVGRTIAVMLVILGLFPMIGGFFTTIPSAVLGGAMTLMFSMIAIAGIRIIITNGL
                     KRRETLIVATSLGLGLGVSYDPEIFKILPASIYVLVENPICAGGLTAILLNIILPGGY
                     RQENVLPGITSAEEMD"
     gene            complement(52757..55627)
                     /locus_tag="NBI74_000338"
     CDS             complement(52757..55627)
                     /locus_tag="NBI74_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase
                     Mo/Fe-S-binding subunit"
                     /protein_id="HCD1930717.1"
                     /translation="MIIHFTLNGAPQELTVNPGENVQKLLFNMGMHSVRNSDDGFGFA
                     GSDAIIFNGNIVNASLLIAAQLEKADIRTAESLGKWNELSLVQQAMVDVGVVQSGYND
                     PAAALIITDLLDRIDAPTREEIDDALSGLFSRDAGWQQYYQVIELAVARKNNPQATID
                     IAPTFRDDLEVIGKHYPKTDAAKMVQAKPCYVEDRVTADACVIKMLRSPHAHALITHL
                     DVSKAEALPGVVHVITHLNCPDIYYTPGGQSAPEPSPLDRRMFGKKMRHVGDRVAAVV
                     AESEDIALEALKLIDVEYEVLKPVMSIDEAMAEDAPVVHDEPVVYVAGAPETLEDDNS
                     HAAQRGEHMIINFPIGSRPRKNIAASIHGHIGDMDKGFADADVIIERTYNSTQAQQCP
                     TETHICFTRMDGDRLVIHASTQVPWHLRRQVARLVGMKQHKVHVIKERVGGGFGSKQD
                     ILLEEVCAWATCVTGRPVLFRYTREEEFIANTSRHVAKVTVKLGAKKDGRLTAVKMDF
                     RANTGPYGNHSLTVPCNGPALSLPLYPCDNVDFQVTTYYSNICPNGAYQGYGAPKGNF
                     AITMALAELAEQLQIDQLEIIERNRVHEGQELKILGAIGEGKAPTSVPSAASCALEEI
                     LRQGREMIQWSSPKPQNGDWHIGRGVAIIMQKSGIPDIDQANCMIKLESDGTFIVHSG
                     GADIGTGLDTVVTKLAAEVLHCPPQDVHVISGDTDHALFDKGAYASSGTCFSGNAARL
                     AAENLREKILFHGAQMLGEPVADVQLATPGVVRGKKGEVSFGEIAHKGETGTGFGSLV
                     GTGSYITPDFAFPYGANFAEVAVNTRTGEIRLDKFYALLDCGTPVNPELALGQIYGAT
                     LRAIGHSMSEEIIYDAEGHPLTRDLRSYGAPKIGDIPRDFRAVLVPSDDKVGPFGAKS
                     ISEIGVNGAAPAIATAIHDACGIWLREWHFTPEKILTALEKI"
     gene            complement(55624..56403)
                     /locus_tag="NBI74_000339"
     CDS             complement(55624..56403)
                     /locus_tag="NBI74_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase
                     FAD-binding subunit"
                     /protein_id="HCD1930718.1"
                     /translation="MIEQFFRPDSVEQALELKRRYQDEAVWFAGGSKLNATPTRTDKK
                     IAISLQDLELDWIDWDNGALRIGAMSRLQSLRDARFIPAALREALGFVYSRHVRNQST
                     IGGEIAARQEESVLLPVLLALDAELVFGNGDTLSIEDYLACPCDRLLTEIIIKDPYRT
                     CATRKISRSQAGLTVVTAAVAITDHDGMRIALDGVASKALRLHDVETQNLEGNALEQA
                     VANAIFPQEDLRGSVAYKRYITGVLVADLYADCQQAGEEAV"
     gene            complement(56454..57782)
                     /gene="ssnA"
                     /locus_tag="NBI74_000340"
     CDS             complement(56454..57782)
                     /gene="ssnA"
                     /locus_tag="NBI74_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative aminohydrolase SsnA"
                     /protein_id="HCD1930719.1"
                     /translation="MLILKNVTAVQLHPAKVQEGVDIAIENDVIVAIGDALTQRYPDA
                     SYKEMHGRIVMPGIVCSHNHFYSGLSRGIMANIAPCPDFISTLKNLWWRLDRALDEES
                     LYYSGLICSLEAIKSGCTSVIDHHASPAYIGGSLSTLRDAFLKVGLRAMTCFETTDRN
                     SGIKELQEGVEENIRFARQIDEAKKAATEPYLVEAHIGAHAPFTVPDAGLEMLREAVK
                     STGRGLHIHVAEDLYDVSYSHHWYGKDLLARLAQFDLIDSKTLVAHGLYLSKDDIALL
                     NQRDAFLVHNARSNMNNHVGYNHHLSDIRNLALGTDGIGSDMFEEMKFAFFKHRDAGG
                     PLWPDSFAKALANGNELMSRNFGAKFGLLEAGYKADLTICDYNSPTPLLADNIAGHIA
                     FGMGSGSVHSVMVNGVMVYEDRQFNFDCDSIYAQARKAAASMWRRMDALA"
     gene            complement(57785..60883)
                     /gene="ygfK"
                     /locus_tag="NBI74_000341"
     CDS             complement(57785..60883)
                     /gene="ygfK"
                     /locus_tag="NBI74_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenate reductase subunit YgfK"
                     /protein_id="HCD1930720.1"
                     /translation="MGDIMRPIPFEELLTRIFDEYQQQRSIFGIPEQQFYSPVKGKTV
                     SVFGETCATPVGPAAGPHTQLAQNIVTSWLTGGRFIELKTVQILDRLELEKPCIDAED
                     ECFNTEWSTEFTLLKAWDEYLKAWFALHLLEAMFQPSDSGKSFIFNMSVGYNLEGIKQ
                     PPMQQFIDNMMDASDHPKFAQYRDALNKLLQDEAFLARHGLQEKRESLQALPARIPTS
                     MVQGVTLSTMHGCPPHEIEAICRYMLEEKGLNTFVKLNPTLLGYARVREILDACGFGY
                     IGLKEESFDHDLKLTQALEMLERLMALAKEKSLGFGVKLTNTLGTINNKGALPGEEMY
                     MSGRALFPLSINVAAVLSRAFDGKLPISYSGGASQLTIRDIFDTGIRPITMATDLLKP
                     GGYLRLSACMRELESSDAWELNHVDVERLNRLAADALTMKYTQKHWKPEERIEVAEDL
                     PLTDCYVAPCVTACAIKQDIPEYIRLLGEHRYADALELIYQRNALPAITGHICDHQCQ
                     YNCTRLDYDSALNIRELKKVALEKGWDEYKQRWHKPAGSGSRHPVAVIGAGPAGLAAG
                     YFLARAGHPVTLFEREANAGGVVKNIIPQFRIPAELIQHDIDFVGAHGVKFEYGCSPD
                     LTVEQLKNQGFHYVLIATGTDKNSGVKLAGDNQNVWKSLPFLREYNKGTALKLGKHVV
                     VVGAGNTAMDCARAALRVPGVEKATVVYRRSLQEMPAWREEYEEALHDGVEFRFLNNP
                     ERFDADGTLTLRVMSLGEPDEKGRRRPVETNETVTLHVDSLITAIGEQQDTEALNAMG
                     VPLDKNGWPDVDHNGETRLTDVFMIGDVQRGPSSIVAAVGTARRATDTILSRENIRSH
                     QNDKYWNNVNPAEIYQRKGDISVTLVNSDDRDAFVAQEAARCLECNYVCSKCVDVCPN
                     RANVSIAVPGFQNRFQTLHLDAYCNECGNCAQFCPWNGKPYKDKITVFSLSQDFDNSS
                     NPGFLVEDCRVRVRLNNQSWVLNIDSEGQFNNVPPELNDMCRIISHVHQHHHYLLGRV
                     EV"
     gene            complement(61205..61783)
                     /gene="mocA"
                     /locus_tag="NBI74_000342"
     CDS             complement(61205..61783)
                     /gene="mocA"
                     /locus_tag="NBI74_000342"
                     /EC_number="2.7.7.76"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor cytidylyltransferase"
                     /protein_id="HCD1930721.1"
                     /translation="MSAIDCIITAAGLSSRMGQWKMMLPWQQGTILDTSIKNALQFCS
                     RIILVTGYRGNELHERYANQSNITIIHNPDYAQGLLTSVKAAVPAVQTEHCFLTHGDM
                     PTLTIDIFRKIWSLRNDGAILPLHNGIPGHPILVSKPCLMQAIQRPNVTNMRQALLMG
                     EHYSVEIENAEIILDIDTPDDFITAKKRYTEI"
     gene            61887..62657
                     /gene="yqeC"
                     /locus_tag="NBI74_000343"
     CDS             61887..62657
                     /gene="yqeC"
                     /locus_tag="NBI74_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417352.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenium-dependent hydroxylase
                     accessory protein YqeC"
                     /protein_id="HCD1930722.1"
                     /translation="MKSIIDSSALFIDLGAQKRPTVISIVGAGGKTSLLFWLAELFQA
                     SGRRVLITTTTHMFMPTSHWPVVFCRDPAMLPHASFISPISFCFHSWKANQGKVQGFT
                     PEAIDALVQRPECDVILIEADGSRGMPLKAPDEHEPCIPKSSCCVIAVMGGHILGAKV
                     STENVHRWSQFADITGLTPDAPLQLSDLVALVRHPQGAFKNVPQGCRRVWFINRFSQC
                     ENAIAQSELLQPLQQHNVEAIWLGDIQEHPAIARRFVN"
     gene            62705..64330
                     /locus_tag="NBI74_000344"
     CDS             62705..64330
                     /locus_tag="NBI74_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF2563 family selenium-dependent molybdenum
                     hydroxylase system protein"
                     /protein_id="HCD1930723.1"
                     /translation="MNIFTEAAKLEEQNCPFAMAQIVDSRGSTPRHSAQMLVRADGSI
                     VGTIGGGMVERKVIEESLQALQERKPRLFHGRMARNGADAVGSDCGGAMSVFISVHGM
                     RPRLVLIGAGHVNRAIAQSAALLGFDIAVADIYRESLNPELFPPSTTLLHAESFGAAV
                     EALDIRPDNFVLIATNNQDREALDKLIEQPIAWLGLLASRRKVQLFLRQLREKGVAEE
                     HIARLHAPVGYNIGAETPQEIAISVLAEILQVKNNAPGGLMMKPSHPSGHQLVVIRGA
                     GDIASGVALRLYHAGFKVIMLEVEKPTVIRCTVAFAQAVFDGEMTVEGVTARLATSSA
                     EAMKLTERGFIPVMVDPTCSLLDELKPLCVVDAILAKQNLGTRADMAPVTIALGPGFT
                     AGKDCHAVIETNRGHWLGQVIYSGCAQENTGVPGNIMGHTTRRVIRAPAAGIMRSNVK
                     LGDLVKEGDVIAWIGEHEIKAPLTGMVRGLLNDGLAVVGGFKIGDIDPRGETADFTSV
                     SDKARAIGGGVLEALMMLMHQGVKATKEVLEVA"
     gene            complement(64371..65303)
                     /gene="arcC"
                     /locus_tag="NBI74_000345"
     CDS             complement(64371..65303)
                     /gene="arcC"
                     /locus_tag="NBI74_000345"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HCD1930724.1"
                     /translation="MSKKIVLALGGNALGDDLAGQMKAVKITSQAIVDLIAQGHEVIV
                     THGNGPQVGMINQAFEAAAKTEAHSPMLPMSVCVALSQGYIGYDLQNALREELLSRGI
                     NKPVATLVTQVEVDANDPAFLNPTKPIGSFFTEQEAELLTKQGYTLKEDAGRGYRRVV
                     ASPKPVDIIEKETVKALVDAGQVVITVGGGGIPVIREGNHLRGASAVIDKDWASARLA
                     EMIDADMLIILTAVEKVAINFGKENEQWLDRLSLSDAERFIEEGHFAKGSMLPKVEAA
                     ASFARSRAGREALITVLSKAKEGIEGKTGTVICQ"
     gene            complement(65351..66736)
                     /gene="hyuA"
                     /locus_tag="NBI74_000346"
     CDS             complement(65351..66736)
                     /gene="hyuA"
                     /locus_tag="NBI74_000346"
                     /EC_number="3.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311773.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-phenylhydantoinase"
                     /protein_id="HCD1930725.1"
                     /translation="MRVLIKNGIAVNADGQAKQDLLIESGIVRQLGTDISPQLPCEEI
                     DASGCYVFPGGVDVHTHFNIDVGIARSCDDFFTGTRAAACGGTTTIIDHMGFGPNGCR
                     LRHQLEVYRGYAAHKAVIDYSFHGVIQHINHAILDEIPMMVEEGLSSFKLYLTYQYKL
                     NDDEVLQALRRLHESGALTTVHPENDAAIASKRAEFIAAGLTAPRYHALSRPLECEAE
                     AIARMINLAQIAGNAPLYIVHLSNGLGLDYLRLARANHQPVWVETCPQYLLLDERSYD
                     TEDGMKFILSPPLRNVREQDKLWCGISDGAIDVVATDHCTFSMAQRLQISKGDFSRCP
                     NGLPGVENRMQLLFSSGVMTGRISPERFVELTSAMPARLFGLWPQKGLLAPGSDGDVV
                     IIDPRQSQQIQHRHLHDNADYSPWEGFTCQGAIVRTLSRGETIFCDGTFTGKAGRGRF
                     LRRKPFVPPVL"
     gene            complement(66789..68000)
                     /locus_tag="NBI74_000347"
     CDS             complement(66789..68000)
                     /locus_tag="NBI74_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgeY family selenium metabolism-linked
                     hydrolase"
                     /protein_id="HCD1930726.1"
                     /translation="MAKNIPFKLILEKAKDYQADMTRFLRDMVAIPSESCDEKRVVHR
                     IKEEMEKVGFDKVEIDPMGNVLGYIGHGPRLVAMDAHIDTVGIGNIKNWDFDPYEGME
                     TDELIGGRGTSDQEGGMASMVYAGKIIKDLGLEDEYTLLVTGTVQEEDCDGLCWQYII
                     EQSGIRPEFVVSTEPTDCQVYRGQRGRMEIRIDVQGVSCHGSAPERGDNAIFKMGPIL
                     GELQELSQRLGYDEFLGKGTLTVSEIFFTSPSRCAVADSCAVSIDRRLTWGETWEGAL
                     DEIRALPAVQKANAVVSMYNYDRPSWTGLVYPTECYFPTWKVEEDHFTVKALVNAYEG
                     LFGKAPVVDKWTFSTNGVSIMGRHGIPVIGFGPGKEPEAHAPNEKTWKSHLVTCAAMY
                     AAIPLSWLATE"
     gene            complement(68058..69254)
                     /gene="dpaL"
                     /locus_tag="NBI74_000348"
     CDS             complement(68058..69254)
                     /gene="dpaL"
                     /locus_tag="NBI74_000348"
                     /EC_number="4.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopropionate ammonia-lyase"
                     /protein_id="HCD1930727.1"
                     /translation="MSVFSLKIDIADNKFFNGETSPLFSQSQAKLARQFHQKIAGYRP
                     TPLCALDDLANLFGVKKILVKDESKRFGLNAFKMLGGAYAIAQLLCEKYHLDIETLSF
                     EHLKNAIGEKMTFATTTDGNHGRGVAWAAQQLGQNAVIYMPKGSAQERVDAILNLGAE
                     CIVTDMNYDDTVRLTMQHAQQHGWEVVQDTAWEGYTKIPTWIMQGYATLADEAVEQMR
                     EMGVTPTHVLLQAGVGAMAGGVLGYLVDVYSPQNLHSIIVEPDKADCIYRSGVKGDIV
                     NVGGDMATIMAGLACGEPNPLGWEILRNCATQFISCQDSVAALGMRVLGNPYGNDPRI
                     ISGESGAVGLGVLAAVHYHPQRQSLMEKLALNKDAVVLVISTEGDTDVKHYREVVWEG
                     KHAVAP"
     gene            complement(69312..70499)
                     /gene="ygeW"
                     /locus_tag="NBI74_000349"
     CDS             complement(69312..70499)
                     /gene="ygeW"
                     /locus_tag="NBI74_000349"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="knotted carbamoyltransferase YgeW"
                     /protein_id="HCD1930728.1"
                     /translation="MKTVNELIKDINSLTSHLHEKDFLLTWEQTPDELKQVLDVAAAL
                     KALRAENISTKVFNSGLGISVFRDNSTRTRFSYASALNLLGLAQQDLDEGKSQIAHGE
                     TVRETANMISFCADAIGIRDDMYLGAGNAYMREVGAALDDGYKQGVLPQRPALVNLQC
                     DIDHPTQSMADLAWLREHFGSLENLKGKKIAMTWAYSPSYGKPLSVPQGIIGLMTRFG
                     MDVTLAHPEGYDLIPDVVEVAKNNAKASGGSFRQVTSMEEAFKDADIVYPKSWAPYKV
                     MEERTELLRANDHEGLKALEKQCLAQNAQHKDWHCTEEMMELTRDGEALYMHCLPADI
                     SGVSCKEGEVTEGVFEKYRIATYKEASWKPYIIAAMILSRKYAKPGALLEQLLKEAQE
                     RVK"
     gene            70978..72756
                     /locus_tag="NBI74_000350"
     CDS             70978..72756
                     /locus_tag="NBI74_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent transcriptional regulator"
                     /protein_id="HCD1930729.1"
                     /translation="MELATTQSVLMQIQPTIQRFARMLASVLQLEVEIVDENLCRVAG
                     TGAYGKFLGRQLSGNSRLLRHVLETKTEKVVTQSRFDPLCEGCDSKENCREKAFLGTP
                     VILQDRCVGVISLIAVTHEQQEHISDNLREFSDYVRHISTIFVSKLLEDQGPGDNISK
                     IFATMIDNMDQGVLVVDADNRVQFVNQTALKTLGVVQNNIIGKPVRFRPLTFESNFTH
                     GHMQHIVSWDDKSELIIGQLHNIQGRQLFLMAFHQSHTSFSVANAPDEPHIEQLVGEC
                     RVMRQLKRLISRIAPSPSSVMVVGESGTGKEVVARAIHKLSGRRNKPFIAINCAAIPE
                     QLLESELFGYVKGAFTGASANGKTGLIQAANTGTLFLDEIGDMPLMLQAKLLRAIEAR
                     EILPIGASSPIQVDIRIISATNQNLAQFIAEGKFREDLFYRLNVIPITLPPLRERQED
                     IELLVHYFLHLHTRRLGSVYPGIAPDVVEILRKHRWPGNLRELSNLMEYLVNVVPSGE
                     VIDSTLLPPNLLNNGTTEQSDVTEASEAHLSLDDAGGTALEEMEKQMIREALSRHNSK
                     KEVADELGIGIATLYRKIKKYELLNT"
     gene            complement(72795..73274)
                     /gene="xdhC"
                     /locus_tag="NBI74_000351"
     CDS             complement(72795..73274)
                     /gene="xdhC"
                     /locus_tag="NBI74_000351"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase iron sulfur-binding
                     subunit XdhC"
                     /protein_id="HCD1930730.1"
                     /translation="MNHSETITIECTINGMPFQLHAAPGTPLSELLREQGLLSVKQGC
                     CVGECGACTVLVDGTAIDSCLFLAAWAEGKEIRTLEGEAKGGKLSHVQLAYAKSGAVQ
                     CGFCTPGLIMATTAMLAKPREKPLTITEIRRGLAGNLCRCTGYQMIVNTVLDCEKTK"
     gene            complement(73271..74149)
                     /gene="xdhB"
                     /locus_tag="NBI74_000352"
     CDS             complement(73271..74149)
                     /gene="xdhB"
                     /locus_tag="NBI74_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase FAD-binding subunit XdhB"
                     /protein_id="HCD1930731.1"
                     /translation="MFDFASYHRAATLADAINLLTDNPQAKLLAGGTDVLIQLHHHND
                     RYRHIVDIHNLAELRGITLAEDGALRIGSATTFTQLIEDPVTQRHLPALCAAAASIAG
                     PQIRNVATYGGNICNGATSADSATPTLIYDAKLEIHSPRGVRFVPINGFHTGPGKVSL
                     EHDEILVAFHFPPQPKEHAGSAHFKYAMRDAMDISTIGCAAHCRLDNGNFSELRLAFG
                     VAAPTPIRCQHAEQTAQNAPLNLQTLEAISESVLQDVAPRSSWRASKEFRLHLIQTMT
                     KKVISEAVAAAGGKLQ"
     gene            complement(74160..76457)
                     /gene="xdhA"
                     /locus_tag="NBI74_000353"
     CDS             complement(74160..76457)
                     /gene="xdhA"
                     /locus_tag="NBI74_000353"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase molybdenum-binding
                     subunit XdhA"
                     /protein_id="HCD1930732.1"
                     /translation="MEAREATATGESCMRVDAIAKVTGRARYTDDYVMAGMCYAKYVR
                     SPIAHGYAVSINDEQARSLPGVLAIFTWEDVPEIPFATAGHAWTLDENKRDTADRALL
                     TRHVRHHGDAVAIVVARDELTAEKAVQLVSIEWQELPVITSSEAALAEDAAPIHNGGN
                     LLKQSTMSTGNVQQTIDAADYQVQGHYQTPVIQHCHMESVTSLAWMEDDSRITIVSST
                     QIPHIVRRVVGQALDIPWSCVRVIKPFIGGGFGNKQDVLEEPMAAFLTSKLGGIPVKV
                     SLSREECFLATRTRHAFTIDGQMGVNRDGTLKGYSLDVLSNTGAYASHGHSIASAGGN
                     KVAYLYPRCAYAYSSKTCYTNLPSAGAMRGYGAPQVVFAVESMLDDAATALGIDPVEI
                     RLRNAAREGDANPLTGKRIYSAGLPECLEKGRKIFEWEKRRAECQNQQGNLRRGVGVA
                     CFSYTSNTWPVGVEIAGARLLMNQDGTINVQSGATEIGQGADTVFSQMVAETVGVPVS
                     DVRVISTQDTDVTPFDPGAFASRQSYVAAPALRSAALLLKEKIIAHAAVMLHQSAMNL
                     TLIKGHIVLVERPEEPLMSLKDLAMDAFYHPERGGQLSAESSIKTTTNPPAFGCTFVD
                     LTVDIALCKVTINRILNVHDSGHILNPLLAEGQVHGGMGMGIGWALFEEMIIDAKSGV
                     VRNPNLLDYKMPTMPDLPQLESAFVEINEPQSAYGHKSLGEPPIIPVAAAIRNAVKMA
                     TGVAINTLPLTPKRLYEEFHLAGLI"
     gene            76871..77626
                     /locus_tag="NBI74_000354"
     CDS             76871..77626
                     /locus_tag="NBI74_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311765.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HCD1930733.1"
                     /translation="MSAGRLNKKSLGIVMLLSVGLLLAGCSGSKSSDTGTYSGSVYTV
                     KRGDTLYRISRTTGTSVKELARLNGISPPYTIEVGQKLKLGGAKSSSSTRKSTAKSTT
                     KTASVTPSSAVPKSSWPPVGQRCWLWPTTGKVIMPYSTADGGNKGIDISAPRGTPIYA
                     AGAGKVVYVGNQLRGYGNLIMIKHSEDYITAYAHNDTMLVNNGQSVKAGQKIATMGST
                     DAASVRLHFQIRYRATAIDPLRYLPPQGSKPKC"
     gene            77705..77778
                     /locus_tag="NBI74_000355"
     tRNA            77705..77778
                     /locus_tag="NBI74_000355"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:77737..77739,aa:Gly,seq:ccc)
BASE COUNT        18938 a        19263 c        20580 g        19148 t
ORIGIN      
        1 ttaccgccgg acggtaccct aaaaataaaa taaggataat ttatgcggct tattgattat
       61 tttccagaat catcaatctc agttatacat tcagcaaaag actggcagga agctatcgat
      121 ttctcgatgg catcattgct ggataaaaac tatatcagcg agaattacat tcaatctatt
      181 aaagactcca ccatcagcaa tggtccttat tatattctcg caccaggcgt ggcaatgcct
      241 catgcgcgac cggaatgtgg ggcgcttaaa accgggatgt ctttgacgtt acttaaacaa
      301 ggcgtttatt tcccggggaa ggacgaaccg attaaattac tcatcgggct ttctgctgcc
      361 gatgccaatt cgcacattgg cgctattcag gcgttaagtg aattactgtg cgaagaagaa
      421 atactcgaac aactcttaac agcatcatca gaaaaacaat tagcggacat tatcagccgc
      481 ggataattac tctctcctct acaggataat attatggaaa acaagtctgc tcgtgcaaag
      541 gtccaggctt ttgggggctt tttgactgca atggtcatcc ccaatatcgg tgcttttatt
      601 gcctggggat ttataacggc attatttatt cccaccggct ggctgccgaa tgaacatttc
      661 gccaaaattg ttggtccgat gattacctat ttattgcccg tgatgattgg ttctaccggt
      721 ggtcatctgg tcggcggtaa acgcggcgcg gtcatgggcg gaattggtac gattggtgtg
      781 atcgtcggcg ctgaaatccc gatgttcctt ggctcgatga ttatggggcc gctcggtggt
      841 ctggtgatta aatatatcga caagtcgctg gaaaaacgca tacctgctgg ttttgagatg
      901 gttatcaata acttctcatt aggtatcgcg gggatgctcc tttgtctgct gggttttgaa
      961 gttatcggtc cggcggtgtt aattgctaat actttcgtca aagagtgtat tgaggcgctg
     1021 gtacatgcgg gttatctgcc tttgctgtca gtcatcaatg aaccggcgaa agtgcttttc
     1081 cttaataatg cgattgatca gggcgtctat tatccgctgg gaatgcaaca ggcttcggtt
     1141 aacggtaaat ccatcttctt catggtggcc tccaacccag gtcctggtct ggggctgctg
     1201 ctggcgttta ccttgtttgg taaagggatg agtaaacgtt ctgcgcccgg ggcgatgatt
     1261 attcacttcc tcggtgggat ccacgaactg tatttcccgt atgtgctgat gaagccgctg
     1321 accattattg ccatgattgc gggcggtatg tctggcacct ggatgtttaa cttactggac
     1381 ggtggtctgg tggctggccc aagtccgggg tctatctttg cttacctggc actgacgccg
     1441 aaaggctcgt tcctggcgac aattgccggt gttacggtag gtaccctggt gtcctttgct
     1501 atcacttcgc tgatactgaa gatggaaaaa acggtggaaa cgaagagcga agatgagttt
     1561 accgagtcag ccaatgcggt taaggcgatg aaacaagaag gtgcattctc gttaagccgt
     1621 gttaagcgta ttgcctttgt ttgcgatgca gggatgggct ccagtgcgat gggcgcaacc
     1681 accttccgta aacgcctgga aaaagcgggg ctggcaatag aagtaaaaca ttacgccata
     1741 gaaaacgtgc ctgcggatgc ggatatcgtc gttactcacg ccagtctgga agggcgcgtg
     1801 aaacgtgtga cggataaacc actgatattg attaataact atattggcga tccaaaactc
     1861 gacactttat ttaatcaatt aaccgctgaa cataaacact gattacagag gtaaaaatga
     1921 aaaccaaagt tgctgctatt tatggcaagc gggatgtccg tctgcgcgaa tttgaactgc
     1981 cagaaattac tgataatgaa ctgttagtga gtgtaatttc tgacagcgtt tgtttatcca
     2041 cctggaaagc ggcgttgctt ggtagtgaac ataaacgcgt ccccgacgat ttagaaaatc
     2101 atccggttat taccgggcat gaatgtgctg gggttgttgt cgaagtgggt aaaaatctca
     2161 ctggcaaata taaaaaaggg cagcgttttg tattgcaacc ggcgatgggg ctaccaagcg
     2221 gatattcagc cggatacagc tacgaatatt ttggtggcaa tgccacctat atgattattc
     2281 ccgaaatcgc gattaattta ggctgtgtat taccgtatca cggctcttat tttgctgcgg
     2341 cgtcgctggc agagcctatg tgctgcatta ttggtgctta tcatgccaat tatcacacca
     2401 cgcaatatgt ttatgagcat cgtatgggcg tcaaacctgg cggcaatatt gccctgctgg
     2461 cgtgtgctgg cccgatgggc attggcgcta tcgattacgc cattaacggc ggcatacaac
     2521 cggcgcgggt ggtggtagtc gatatcgacg acaaacggct ggcgcaggtg cagaagttgc
     2581 tgccggtgga gctggcggcc agtaaaggca ttgagctggt gtatatgaat accaaaggga
     2641 tgagcgatcc ggtccagacg ctgcgggcgc tgacggatga tgccggattt gatgacgtat
     2701 ttgtctatgc agcggtgcct tcagtcgtcg agatggctga tgaattactg gctgaagatg
     2761 gctgcctgaa cttctttgcc ggtccgacgg ataaaaactt caaagtgccg tttaatttct
     2821 acaacgtcca ttacaacagt acgcacattg tcggcacgtc tggcggttcc acggacgaca
     2881 tgaaagaggc gattgccctt agcgccacgg ggcagttaca gccgtcgttt atggtgaccc
     2941 atattggtgg cctggatgcg gtgccagaaa ccgtgctcaa tctgccggat atccctggcg
     3001 gtaaaaaact catttataac ggcgtgacca tgccgctgac cgccattgcc gattttgccg
     3061 aaaaaggcaa aaccgatccg ctgtttaaag agctggcgcg gctggttgag aaaacgcacg
     3121 gtatctggaa tgaacaggcc gagaaatatc tgctggcaca atttggcgtt gatattgggg
     3181 aggccgcgca atgatgtccc tggcgtggcc attatttcgc gttacggaac aggcagcgtt
     3241 ggctgcctgg ccgcaaaccg gctgtggcga caaaaataaa attgatggcc ttgcggtcac
     3301 cgcgatgcgc caggcattaa acgacgttgc ttttcgtggg cgagtggtta tcggcgaggg
     3361 ggaaattgac catgcgccga tgctgtggat tggcgaagag gtgggcaaag gcgatgggcc
     3421 agaagtcgat atcgcggtcg atcccattga aggcacgcgg atggtggcga tggggcagag
     3481 caatgcgctg gcggtcatgg ctttcgcccc tcgagatagc ctgttacatg cgcccgatat
     3541 gtatatgaaa aagctggttg ttaatcgact ggctgccggg gcgatagatc tgtctttgcc
     3601 tctggcggac aacttgcgta acgtggcgcg ggcgttaggt aaaccgctgg acaagctgcg
     3661 catggttacg ctggataaac cgcgcctgag tgccgcaatt gaggaagcaa cacagctcgg
     3721 cgtgaaggtt tttgccctgc cggatggcga tgtcgccgcc agcgtgctga cttgctggca
     3781 ggataatcct tacgatgtga tgtacaccat cggtggcgca ccggaaggtg ttatttctgc
     3841 ctgtgccgtt aaagcattag gcggggatat gcaggccgag ctgattgact tttgtcaggc
     3901 taaaggggat tacacggaaa atcggcagat tgcagagcag gagcgcaagc gttgcaaggc
     3961 aatgggcgtt gacgttaacc gtgtatactc gctcgatgaa ctggtgaggg ggaacgatat
     4021 cctctttagc gctacgggcg tgacgggcgg cgagttggtg aacggtatcc aacagacggc
     4081 gaatggggtg cggacgcaga cattactgat cggcggcgcg gaccaaacgt gtaatataat
     4141 agactccctg cattgatggt gatctatcgg aagaccaatg gcgacgctga cagaagatga
     4201 tgtgcttgag caactgaatg cacaggacaa tttattttca tttatgaaaa ctgcgcattc
     4261 tattttgctc caggggatac gccagtttct gccgtcgctg tttgtcgata acgatgaaga
     4321 gatcgtcgaa tatgcagtga agccgttact cgcccaaagc ggcccgcttg acgatattga
     4381 tgttgcgctg cgtttgattt atgcgctggg gaaaatggat aaatggctgt acgccgatat
     4441 cacgcatttt tcccagtttt ggcattacct gaacgaacag gatgaaacgc ccagatttgc
     4501 tgatgacata acctgggatt ttatcagcaa cgtcaatagc ataacccgca atgcgacgct
     4561 ctatgacgcg ttgaaagcga tgaagttcgc cgatttcgcc gtctggtcag aggcgcgttt
     4621 tagcgggatg gtcaaaacgg cgctgacgct ggcagtaacg accaccttaa aggaattaac
     4681 gccgtgaaaa tcgaactgac ggtgaatgga ctaaaggtac aggcgcagta ctctgatgat
     4741 gaaattgaaa atgtacataa accattgctg cgtatgctgg cggccttaca gacagtaaat
     4801 ccgcagcggc gtacggtggt ttttctttgc gctccgccgg ggacgggtaa atctacccta
     4861 accaccttct gggaatatct cgcgcaacaa gacccggaac tgcccgctat tcagacgctg
     4921 ccgatggatg gttttcacca ttacaatagc tggctggatg tgcatcaatt gcgacccttc
     4981 aaaggcgcac cagagacatt caacgttgcg aaactggcgg aaaatctgtg ccgggttgtg
     5041 gaaggggatt gtacgtggcc gcagtacgat cgacaaaagc atgatcctgt tgaagatgtg
     5101 ttgcacgtta ccgcaccact cgtcatcgtc gaaggaaact ggttgttgtt ggacgacgag
     5161 aagtggtgcc agcttgcgca attttgtgat ttctcgatct ttatcaaagc tcccgcatct
     5221 gcgttgcggg agcgtctggt ggggcgcaag ctggcgggag ggttatcgct ggcagacgca
     5281 gaagcctttt atgaccgtac cgacgggccg aatgttcgcc gggtgctgga agagagtctg
     5341 cctgcaaatt tgaccttaat gatgaccgct acgggtgaat atcgtctgat ggattaacgt
     5401 tcctgattac accatcttcc agtcgcacca cacgagagaa atgacgccgg gtaaatgcgg
     5461 cgtcatgact gattgctacg acgctcgtgc ctcgctggcg gcacttttcc agccagcttt
     5521 caaacacgct caaccaattt tcgtcaaaat cccgactcgg ctcgtccagt agcaatagtg
     5581 gcggagagag tgcttccagg caggcgacgg cgaccatccg tcgctgcgca ctatgcagat
     5641 ctaacggatg cgcgctggca gtgtctgcta actgacagca ttgcagggca gcattggtac
     5701 gttgagtgat ttcatccgcc gggcattttt gcagtatcaa accaaatgcg atttcgtcag
     5761 caacggtgct gtgaaaaagc tgattttccg cttcctgaaa cagtacgcca atctttgccg
     5821 cgcgctggcg gtttttcagg tttttcatcg cttgttgttg cagcataacc gtaccggctg
     5881 tgggcgtaag aagacctgcc attactcgta acagcgtcga ttttcccgcg ccattgtcgc
     5941 cggtcagcgc cagccattca ccttgtttga gttgcagaga aatgtcgcaa agacaatccg
     6001 tagccgcgcc aggccagcga taagatatct gatttaacgt taacatagtg gctggagcgc
     6061 tccgttttgc aattgccacg cttgctggca gtggttcagg aaaggtgtgt gatgacgttc
     6121 aaaaagaata atcagcgaac cacgttcaag cgcccagtgt tgtaagcgct gcaacagcat
     6181 ttcgcgggcc tgcggcgtca ggcggctaaa ggcttcatca agaattaaga gctttggctg
     6241 catcgcgatg gcgcaggcga tgaccacacg ctgtgtctct ccaccggaga gtgttgccgg
     6301 gtgacgatgg cgcaacggct ggcactcggt cagggccagg gcggcgtcaa tccgcgccat
     6361 aatctctttt tcagcaagac acagattttc tggcccaaag gcaacttctt cttccacgct
     6421 aaaggtacag ccggagagct gcaaataggg cgattgctgg acgagctgga tggtggcgga
     6481 ttgttcatta agcgggagac gcccaatggg cgtaccgaga agcgtccccg tgccggttat
     6541 ctctcctggt agaaagtccg gataccagcc cgccatcagc tgtgccagcg tacttttgcc
     6601 actgccgttg tcaccaaaga tggcgaccat ccccggtttg acatagtgaa agtcataaca
     6661 gaacggagga tgcgttgagt gtgtcgggca atagcgaaac tgttctaacg taaccatatc
     6721 cacgctccga attcagccag cattagcaat atcatggcgt aacgtgctac gcattgtagg
     6781 gtgctatcgg ctggtgccca taacgtagtg cgcctattgt tgattcgaaa tgcccgcata
     6841 tccagcgccg caccacggac agcaagatcg tttagcgcat tgtgtgttag cggaataatt
     6901 aacgcgggca tcgcacgtaa acgctgatac cagccctcat ccagcggcac gccgcgggcg
     6961 cactgcgctt catgaataat cgccaattgc cgttttaact gctcaacgac caataacggc
     7021 ccggcaaaca gataagcgac gcccggcggc aggcgagaag caaacaaagc gcggataaaa
     7081 cgttgcacgg ggacgtactg catccacaac tgtgaggttg aaacaatcgc cagaatccgc
     7141 aaccagagcg taatagcatg cgcccaccgt tctggagagt gtggcgtgcc gcttagccat
     7201 tctgtcagcc agccaccgtg caccagccac agtccagccc ccagcgagaa catcagccag
     7261 acgacatatt ttgcccgccg ccgcgtggcc tttagcgcaa taaggcagaa aaaggtcgcc
     7321 gcgctataga ctggcaatat tgtctgtgta ggcaggataa gcgtggtgca ggccgccagc
     7381 gcccacagcg ttaaggatgt aaaaggatgc atttagcgca cggcagccat tgccggaaaa
     7441 tggcgcgtgg tgcgaatcgg cagctgacgc agtaaaagcc agactatcac cgctgtaaga
     7501 attttatcga ccaggttcgc gcctatcacc gtaatcgcca ccgactccac caggttttgc
     7561 cccatcgagt gcatccaggc aacgaacaaa tcggctccgc ttcctgtcac gccgccgaac
     7621 agcgcagtac gcagcggtac agcgacgacc gttaccgcaa gcgtaataat cacgccgcta
     7681 acaacgactt ttggcagggt gcgaaaccaa cccgctcgcg ccagccaacc ggcgaccagg
     7741 ccgatgacca tcgcgacggg cgcaaatgcg gcggcgatcg ggtcggtcag caatccccag
     7801 agtaaattgg tcagtaaacc ggtcagcatc ccgattatcg gaccaagcag taccgcgcta
     7861 ataagtgtgc cgatggagtc gagaaaaata ggtaatttaa ccatgctggc cagttgcccg
     7921 ccgatcatat tgatggcgat agagataacg atcaacacca gtgcctgact ggaaaagtgg
     7981 cgacgcgcca taagaaaacc tctctgaaaa tagttattta acgggggaaa tatgcaactc
     8041 gtcgtaacca gaaagcgcgc aaagctggcg gctaaacgcc agttcctcaa ggcagccgat
     8101 caccagttca agcgtggtcc gcacggtaca gccgggcatc atatgaccgc caagcatcgt
     8161 gccgtgtgga tcggaaacgc agagatgaag atgttcacca ctctgttcca gcgtgccgtt
     8221 caatgcgatg acttcgaact taccgcttag cagggcagtg ttttcttgcc cggcatagcg
     8281 taaggcgacg tcagtcagac tgccggtaca acctgcaatc cacgcggcgt gaagttgttg
     8341 ttgctgcgcg aaggcgcgta attgggagag aacttcctgc ccggggagca ggcgcagcgc
     8401 gtaaaagcgt gcggaggaag aatatgggta gggcaatgtc atcattggag tactccgaaa
     8461 ctcggacgta ttgcggataa tatcgacgct gcatcagtca tattctgata taagtcaggg
     8521 tttgtgaccg cacaacgaaa agatattgct acgcttattc gtgattctgc aaagtggaat
     8581 aaattccaca ggggcgggga tgaaaacgtg acataagtca gataattgtc ttctttgact
     8641 ggacaaacat tccttttatt ccacgtttcg cttatcctag ctgaagcgtt tcagtcgatt
     8701 aaatgttcga caattaacca atcagtcgca gtttgcgaca ggtaaggttt ccccgaacga
     8761 tttgctggat tactctgtct ggcaaatttg ctcagggaaa accttgcagg agatctatga
     8821 ccgtacgcgt agcgataaat ggcttcggtc gcatcgggcg taatgtggtt cgtgctttgt
     8881 atgaatccgg acgccgtgcg gaaattaccg tggtggcaat caacgaactg gcggatgctg
     8941 cgggcatggc gcatttgttg aaatatgaca ccagccatgg ccgttttgca tgggaagtac
     9001 gacaggaacg cgatcaactt tttgttggtg atgacgccat ccgtgtattg catgaacgtt
     9061 cactgcaatc gctcccctgg cgtgaacttg gcgttgatgt agtcctcgac tgcaccggcg
     9121 tatatggctc ccgcgagcat ggtgaagcac acattgccgc cggggctaaa aaagtgctct
     9181 tttcacatcc tggcagtaac gatctcgacg cgaccgttgt ttatggcgtc aatcaggatc
     9241 aacttcgtgc ggaacaccgc atcgtttcta atgcttcctg taccacgaat tgcataattc
     9301 ccgtcatcaa attgttagat gatgcgtacg gtattgagtc tggcactgtg accacaattc
     9361 actccgccat gcatgatcaa caggttattg atgcatacca tcctgacctg cgtcgcactc
     9421 gggcagccag tcagtcgatc attccggtcg atactaaact ggccgccggt atcacacgat
     9481 tttttccgca atttaacgat cgctttgagg cgattgcggt acgtgtgcca accataaatg
     9541 tgacggcaat cgatttaagc gtgacggtga agaaacctgt aaaagccaat gaagtcaacc
     9601 tgttgctgca aaaagcagca caaggtgcat ttcatggtat agttgactat acggaattgc
     9661 cgttggtctc tgtagatttt aaccacgatc cgcacagtgc cattgtcgat ggcactcaaa
     9721 cccgggtcag tggcgcacac ctgatcaaaa cgttggtctg gtgcgataac gaatggggct
     9781 ttgctaaccg aatgctcgac acgacgttag ctatggctac tgttgctttc aggtaagacg
     9841 caagcagcgt ctacaaaact tttagaatca acgagaggat tcaccatgtc tgtaattaag
     9901 atgaccgatc tggatcttgc tgggaaacgt gtatttatcc gtgcggatct gaacgtacca
     9961 gtaaaagacg ggaaagtaac cagcgacgcg cgtatccgtg cttctctgcc gaccatcgaa
    10021 ctggccctga aacaaggcgc aaaagtgatg gtaacttccc acctgggtcg tcctaccgaa
    10081 ggcgagtaca acgaagaatt ctctctgctg ccggttgtta actacctgaa agacaaactg
    10141 tctaacccgg ttcgtctggt taaagattac ctcgacggcg ttgacgttgc tgaaggtgaa
    10201 ctggttgttc tggaaaacgt tcgcttcaac aaaggcgaga agaaagacga cgaaactctg
    10261 tccaaaaaat acgctgcact gtgtgacgtg ttcgtaatgg acgcattcgg tactgctcac
    10321 cgcgcgcagg cttctactca cggtatcggt aaattcgctg acgttgcgtg cgcaggcccg
    10381 ctgctggcag ctgaactgga cgcgctgggt aaagcactga aagaacctgc tcgcccgatg
    10441 gtggctatcg ttggtggttc taaagtatct accaaactga ccgttctgga ctccctgtct
    10501 aaaatcgctg accagctgat cgttggtggt ggtattgcta acacctttat tgcggcacaa
    10561 ggccacgatg tgggtaaatc cctgtacgaa gctgacctgg ttgacgaagc taaacgtctg
    10621 ctgaccacct gcaacatccc ggttccgtct gatgttcgcg tagcaaccga gttctctgaa
    10681 actgcaccgg ctaccctgaa atctgttaac gatgtgaaag ctgacgagca gatcctggat
    10741 atcggtgatg cttccgctca ggaactggct gaaatcctga agaatgcgaa aaccattctg
    10801 tggaacggtc cggttggcgt gttcgaattc ccgaacttcc gcaaaggtac tgaaatcgtg
    10861 gctaacgcta tcgcagacag cgaagcgttc tccatcgctg gcggcggcga cactctggca
    10921 gcaatcgacc tgttcggcat tgctgacaaa atctcctaca tctccactgg cggcggcgca
    10981 ttcctcgaat tcgtggaagg taaagtactg cctgcagtag cgatgctcga agagcgcgct
    11041 aagaagtaaa aaatcacagg gcagggaaac ctgcccttgt ttcagcgcgc ttttagagca
    11101 cgcacttttt catactctaa ataattcgag ttgcaggaag gcgacaagag agtgaatccc
    11161 aggagcttac ataagtaagt gattggggtg agcgaacgca gacgcagcac atgcaacttg
    11221 aagtatgacg agtataaggc ccgacgatac aggacaagag acatgtctaa gatttttgat
    11281 ttcgtaaaac ctggcgtaat cactggtgat gacgtacaga aagttttcca ggtagcaaaa
    11341 gaaaacaact tcgcactgcc agcagtaaac tgcgtcggta ctgactccat caacgccgtg
    11401 ctggaaaccg ctgctaaagt taaagcgccg gttatcgttc agttctccaa cggtggtgct
    11461 tcctttatcg ctggtaaagg cgtgaaatct gacgttccgc agggtgctgc tatcctgggc
    11521 gcgatctctg gtgcgcatca cgttcaccag atggctgaac attatggtgt tccggttatc
    11581 ctgcacaccg accactgcgc gaagaaactg ctgccgtgga tcgacggtct gctggacgcg
    11641 ggtgaaaaac acttcgcagc taccggcaag ccgctgttct cttctcacat gatcgacctg
    11701 tctgaagaat ctctgcaaga gaacatcgaa atctgctcta aatacctgga gcgcatgtcc
    11761 aaaatcggca tgactctgga aatcgaactg ggttgcaccg gtggtgaaga agacggcgtg
    11821 gacaacagcc acatggacgc ttctgcactg tacacccagc cggaagacgt tgattatgcg
    11881 tataccgagc tgagcaaaat cagcccgcgt ttcaccatcg cagcgtcctt cggtaacgta
    11941 cacggtgttt acaagccggg taacgttgtt ctgactccga ccatcctgcg cgactctcag
    12001 gaatatgttt ctaagaaaca caacctgccg cacaacagcc tgaacttcgt attccacggt
    12061 ggttccggtt ctactgctca ggaaatcaaa gactccgtaa gctacggcgt agtgaaaatg
    12121 aacatcgata ccgataccca atgggcaacc tgggaaggcg ttctgaacta ctacaaagcg
    12181 aacgaagctt acctgcaggg tcagctgggt aacccgaaag gcgaagatca gccgaacaag
    12241 aaatactacg atccgcgcgt atggctgcgt gccggtcaga cttcgatgat cgctcgtctg
    12301 gaaaaagcat tccaggaact gaacgcgatc gacgttctgt aagtcattct tttctgctta
    12361 tctcaaggcc cgctctgcgg gtcttttttt cgccaaaagc aaggtgatgc agatgagaaa
    12421 tctgtgatct atttggcaaa attatgcttt attgtttacc cttgtcagac tgcccgccat
    12481 aaggcggcgg agtgtatttc tccattttga gtcagttgaa aaggaatatt gaatggaaga
    12541 tttgaatgtt gtcgatagca taaacggcgc gggaagctgg ctggtggcta accaggcgct
    12601 gctgctaagt tatgcagtaa acatcgtggc ggcactcgcg atcatcatcg ttggtttgat
    12661 tatcgcgcgg atgatttcca acgcggtgaa tcgcctgatg atctcccgta aaatcgatgc
    12721 cactgttgct gattttcttt ctgcattagt ccgttacgga attatcgcct ttacgctaat
    12781 cgctgcactg gggcgcgtgg gcgtacaaac agcgtcagtc attgctgtac tcggtgccgc
    12841 aggcttagct gttggtctgg ctttgcaggg gtcactgtct aacctggccg ctggcgtgtt
    12901 acttgtcatg ttccgcccgt tccgtgccgg tgaatatgtt gacctcggcg gcgtagccgg
    12961 cactgtgctg agtgtgcaga ttttctccac caccatgcgt actgcagacg gtaaaattat
    13021 cgttattccg aatggtaaaa ttattgccgg gaatattatt aacttctccc gcgagccagc
    13081 tcgccgtaac gaatttatta ttggcgtggc gtatgattcc gatatcgatc aggttaagca
    13141 gatcctgacc gatattatcc agtctgaaga tcgcattttg aaagatcgcg aaatgactgt
    13201 gcgtctgaac gaacttggtg catcgtcgat taatttcgtg gtccgagtct ggagcaacag
    13261 cggcgatctg caaaacgtgt actgggatgt gctggagcgt attaaacgtg aatttgatgc
    13321 cgccggtatc agcttcccgt atccgcaaat ggatgtgaac tttaagcggg tgaaagaaga
    13381 caaagctgcg taatcaacgc tgaggccaga taatactctc tggcctctct cttattagtt
    13441 tttctgattg ccaattaata ttatcaattt ccgctaataa caatcccgcg atatagtctc
    13501 tgcatcagat acttaattcg gaatatctaa cgtgttttct tattactttc aaggtcttgc
    13561 acttggggcg gctatgatcc taccgctcgg tccacaaaat gcttttgtga tgaatcaggg
    13621 cattcgtcgt cagtaccaca ttatgattgc cttactttgt gctatcagcg atttggtcct
    13681 gatttgtgcg gggatttttg gtggcagcgc gttattgatg cagtcgccgt ggttgctggc
    13741 gctggtcacc tggggcggcg tagccttctt gctgtggtat ggttttggcg cttttaaaac
    13801 agcaatgagc agtaacattg agttagccag tgccgaagtc ctgaaacaag gcagatggaa
    13861 aattatcgcc accatgttgg cagtgacctg gctgaatccg catgtttacc tggatacttt
    13921 tgttgtgctg ggcagccttg gcgggcaact tgatgtggaa ccaaaacgtt ggtttgcgct
    13981 cgggacaatt agcgcctctt tcctgtggtt ctttggtctg gctattctcg cagcctggct
    14041 ggcaccgcgc ctgcgcacgg caaaatcaca gcgcattatc aatctggtag tgggatgtgt
    14101 tatgtggttt attgccttgc agctggcgag agacggtatt gctcatgcac aagccttgtt
    14161 cagttaggcg ctatctgatg gaaaaataaa acagaggcgc taagcttgcc tccagaggtc
    14221 ctgaattttg ggcaagtcaa ttaacacgga gagactaacg tgaagttcaa agttatcgcc
    14281 ctggcggcat taatgggtat tagcgggatg gcagcgcagg ctaacgaatt gccggatgga
    14341 ccgcatattg tcacctccgg tacggcaagc gtggatgcgg tgccagacat tgccactctt
    14401 gcgattgaag ttaacgtggc cgcgaaggat gccgctactg ccaagaaaca ggcagatgag
    14461 cgcgtcgcac aatacatttc cttccttgag ctcaatcaga tcgcgaaaaa agatatcagc
    14521 tcagcgaact tacgcactca gccagattat gattatcagg atggtaaaag tatcctgaaa
    14581 ggctaccgcg cagtgagaac ggtggaagtc acgctccgtc agttagacaa actgaattcc
    14641 ttgctggacg gcgcgctaaa ggcgggtctt aacgaaattc gttctgtgtc gctgggcgtg
    14701 gcgcagccgg atgcctataa agacaaagcg cgtaaggcag cgattgataa cgcgattcat
    14761 caggcacagg aactggcgaa tggctttaat cgtaaactgg ggccggtata tagcgtgcgc
    14821 taccatgttt ccaactatca gcccagccca atggtgcgga tgatgaaagc tgatgctgca
    14881 ccggtgtccg cacaggaaac ttacgagcag gccgctattc agtttgatga tcaggtcgat
    14941 gtagtcttcc agttagaacc tgtggatcaa caacctgcta aaacacctgc agcacaataa
    15001 tacttgtagc ccgacgtatt cgtcgggcta atgcctttac ccgatattgc ctcctttttc
    15061 tgtaattttc tgtaaaacca tgacgtaatc acctatcttt gctcgcggtt tcatcaagga
    15121 taggctgact gaaacggtta agcggacaag gatgcgggag aggggggatg gacattttta
    15181 tttcgaaaaa gatgcgcaat ttcattttat tagcgcaaac caataatatt gcccgggcgg
    15241 cagaaaaaat ccatatgacg gcttcgccat ttggcaaaag cattgccgcg ctggaagaac
    15301 aaattggcta tacgctattt acccgcaaag ataataatat cagcctcaat aaagccggac
    15361 aggaactgta tcaaaaactg ttcccggttt atcaacgact ttctgcgatt gataatgaaa
    15421 tccataactc aggacgtcgt taacgagaaa ttgtgatagg catagataat acttatccga
    15481 caattatttt tgatcagttg attagtctcg gagataagta cgaaggcgtg accatccagc
    15541 cagttgagtt cagcgaaaat ggcgttatcg ataatctttt tgaccgtcag ttagatttta
    15601 ttatctcccc acagcatgta tctgcacgag ttcaggaact ggaaaacctg actatcagtg
    15661 aactaccacc gctgcgattg ggctttctcg tttctcgccg ttatgaaggg cgacaggagc
    15721 aagagctgct tcaggagctg ccctagctac aaatgcgctt tcaaaaccgg gctaacttcg
    15781 aagcaatgat tgatgcgaat atgcgtcctt gtgggatcaa tccaaccatt atttatcgtc
    15841 cctatagttt tatggctaaa atcagcgccg tggagcgggg acattttctg acggtgatcc
    15901 cgcattttgc ctggcgactg gtgaatccgg caacgctgaa atattttgat gcgccgcaca
    15961 ggccgatgta tatgcaggaa tatctttatt caatcagaaa tcatcgttat accgccacga
    16021 tgcttcagca tattgctgaa gatcgtgacg ggacaaacca ttaacccagc atcgatccgg
    16081 ttgcaattaa attacggtgt aagtcgaaga cgtggctaag atcgtggtga atatgtccgc
    16141 cggacgcatt ttccagatat cgatgcagat aatcccgata catgggatgt gcacaatgat
    16201 caatggtggt gcggataacg ttgttatacg gatcctgtgc gccaaatcca ctgaccgctt
    16261 cgctccataa ataacgtgcc gcacgcaaca tggcgacgtt cataaacaga tccatgccaa
    16321 tgccgaagaa aaacgacagg cgaggggcga agtcatcaat tttcagcccg gcggagattg
    16381 ctgctttgat gtactcaatc ctatcggcga gcgtaaatgc tacctgctgc acgcagttgg
    16441 cacccgcttc acccatgtgg taaccgctga tactgatggt attaaatcgc ggcatgttgc
    16501 cggaacacca ggcgatgatg tctgcgataa tacgcattga cggttttggt gggtaaatat
    16561 aggtgttgcg gcaaagatac tctttgagga tatcgttttg aatggtgccg gtcagtttat
    16621 cggatgtaac accttgctct tctgcggcga cgatataaaa cgccagtact ggtagcactg
    16681 cgccattcat ggtcatcgaa accgacattt tatccagcgg gatctggtcg aacaggactt
    16741 ttatatcttc cacggtgtcg atagcgacgc ccgctttgcc gacgtcgccc gccacgcgtg
    16801 ggttatcgga gtcgtagcct cggtgggtgg ccaggttacg gcgataaaaa gcgttggact
    16861 cttttgctgt cgagaaacca gcatactgac ggatggtcca cggttgggcg gtatacatag
    16921 tggcacgcgg gccacgaacg taggggggca aaccaggaag ggtacccgtc acctccagat
    16981 tatcgagatc ggcttcggta tacagcggct tgatggcgat cccttccgcg gtttgctgaa
    17041 ccagcgagtc gacagttttc tcccgacggc tcaattcctt gttggcaagc tgttgccact
    17101 cctgcacgtt agacattatt tgctccgtaa aacgcaatca aaatgagggt atttgcgctt
    17161 acggtgagct ttttacgtgg gtttgtcgcc agcaaaaaaa tgcattcaga cgtcgttttt
    17221 tgccgcctga gatttgtcta ttttgcgatg tgattcaagg aatacaaaaa gaagtgagga
    17281 taacgcctga tatgtgctat caggcgttat ttgatggatt aatcctgacg aagaactttg
    17341 tgaccataat cgagcaacgc atcagtgact ttacgcatca tgcggctttc aggagcaaag
    17401 cggtgccagt agagcatccg gcgttgaaat agccctggcg ttaagtcaat cagttcgccg
    17461 ctggccagct ctttctcgat ttgcaggtgc gggatcatac agcaggtggt gccctgacga
    17521 gcaagttgca cgaacgcttc cgaagaatta acgatatggc agggtacgct gccaggaggc
    17581 agatcgaaat tttgctgcaa aaaggcctgg tgcatatcgt caagatggtc aaacgcgacc
    17641 actggcgctt tcagtaatgc cgaacgcgtt acgccgttag ggaaatattt ttcggcaaat
    17701 ggttttgagc tgacgaacag atagtcgagc gcaccgagtt tatcgacaag acaactcggc
    17761 agcgcctgat gttgaatact cactgcgccg accacttcgc cgcgacgcag acgttcctga
    17821 gtgcgggttt catcttctac ctgcaagttg aggcggatgg gcgaatcagc caacacagga
    17881 gccagtgccg gaagcaacca cgtcgccaga ctgtcggcgt tgaccgccag tgaaagcagc
    17941 agcggagtcg aaccggtttg ttcatcgccc agccactctt cttccagcag ctccacctgg
    18001 cgtagtagcg ccaacagttt ttgcccttgt tccgtcgggc gcggcggtac ggtacgcact
    18061 aacaacggct gcccgaacat attttccagt tgcttaatgc gctgtgagac ggctgattgt
    18121 gtaatgcaca gcttttgtgc cgcgcgctca aatcctcgtt cacgtatcac cgcatccagt
    18181 gcctgtaatg ttctgtagtc cgggcgtttc attgctgttg tctgctcctg gtaaatgctt
    18241 tttcctgcac tatgacacaa ttttgtgtcg ggttgcatat accgaagccg ttggcgggta
    18301 acatttgtat cagtggttaa cgcgcagctt aaaagatcag agatttgcgc taatgcattt
    18361 cgctcagcga gttcgcgccc tggttgtgtt gaatggcgtg gcgttattgc ctcagtttgc
    18421 ctgtaaacag gggcttgcga acggggaact ggtgcgcctg ttcgcaccgt ggagcggcat
    18481 acccagaccg ttgtatgctt tatttgcggg gcgaaaaggg atgcccgcca ttgcgcgata
    18541 ttttatggat gagttaacca cgcggcttgc caacggggtc tgaatcgctt tttttgtata
    18601 taatgcgtgt gaaatttcat accacaggcg aaacgatcat gacgcaggat gaattgaaaa
    18661 aagcagtagg atgggcggca cttcagtatg ttcagcccgg caccattgtt ggtgtaggta
    18721 caggttccac cgccgcacac tttattgacg cactcggtac aatgaaaggc cagattgaag
    18781 gggccgtttc cagttcagat gcttccactg aaaaactgaa aagcctcggc attcacgttt
    18841 ttgatctcaa cgaagtcgac agccttggca tctacgttga tggcgcagat gaaatcaacg
    18901 gccacatgca aatgatcaaa ggcggcggcg cggcgctgac ccgtgaaaaa atcattgctt
    18961 cggttgcgga aaaatttatc tgtattgcag acgcttccaa gcaggttgat attctgggca
    19021 aattcccgct gccagtagaa gttatcccga tggcacgtag tgcagtggcg cgccagctgg
    19081 tgaaactggg cggtcgtccg gaataccgtc agggcgtagt gaccgataac ggcaacgtga
    19141 tcctcgacgt ccacggcatg gaaatccttg acccgatagc gatggaaaac gccataaatg
    19201 cgattcctgg cgtggtgact gttggtttgt ttgctaaccg tggcgcggac gttgcgctga
    19261 ttggcacacc tgacggtgtc aaaactattg tgaaatgatc tgacggggga acctcccccg
    19321 ttaaaaaaat tctcttcatt aaatttggtg acatgtgtca cgtttttacc aggcaattgt
    19381 cgattgctct aaataaatcc tctaaaccag catattcatc caagaaatac ttttgcgtga
    19441 tatttcctca acatcgcgac gcaaacgttc atattgccgc aatattattt tttgatatgt
    19501 tgaaaggcgg atgcaaatcc gcacacaaca tttcaaaaga caggattggg taaatggcaa
    19561 aggtatcgct ggagaaagac aagattaagt ttctgctggt agaaggcgtg caccaaaagg
    19621 cgctggaaag ccttcgtgca gctggttaca ccaacatcga atttcacaaa ggcgcgctgg
    19681 atgatgaaca attaaaagaa tccatccgcg atgcccactt catcggcctg cgatcccgta
    19741 cccatctgac tgaagacgtg atcaacgccg cagaaaaact ggtcgctatt ggctgtttct
    19801 gtatcggaac aaaccaggtt gatctggatg cggcggcaaa gcgcgggatc ccggtattta
    19861 acgcaccgtt ctcaaatacg cgctctgttg cggagctggt gattggcgaa ctgctgctgc
    19921 tattgcgcgg cgtgccggaa gccaatgcta aagcgcatcg tggcgtgtgg aacaaactgg
    19981 cggcgggttc ttttgaagcg cgcggcaaaa agctgggtat catcggctac ggtcatattg
    20041 gtacgcaatt gggcattctg gctgaatcgc tgggaatgta tgtttacttt tatgatattg
    20101 aaaacaaact gccgctgggc aacgccactc aggtacagca tctttctgac ctgctgaata
    20161 tgagcgatgt ggtgagtctg catgtaccag agaatccgtc aaccaaaaat atgatgggcg
    20221 cgaaagagat ttcgctaatg aaacccggct cgctgctgat taatgcttcg cgcggtactg
    20281 tggtggatat tccagcgctg tgtgacgcgc tggcgagcaa acatctggcg ggggcggcaa
    20341 tcgacgtatt cccgacggaa ccggcgacca atagcgatcc atttacctct ccgctgtgtg
    20401 aattcgacaa tgtccttctg acgccacaca ttggcggttc gactcaggaa gcgcaggaga
    20461 atattggcct ggaagttgcg ggtaaattga tcaagtattc tgacaatggt tcaacgctct
    20521 ctgcggtgaa cttcccggaa gtctcgctgc cactgcacgg tggtcgtcgt ctgatgcaca
    20581 tccacgaaaa ccgtccgggt gtgctgactg cgctcaacaa aatttttgcc gagcagggcg
    20641 tcaacatcgc cgcgcaatat ctgcaaactt ccgcccagat gggttatgta gttattgata
    20701 ttgaagccga cgaagacgtt gccgaaaaag cgctgcaggc aatgaaagct attccgggta
    20761 ccattcgcgc ccgtctgcta tactaaatcc ctttctctga aaatcaacgg gcaggtcact
    20821 gacttgcccg ttattttatc ccttctccac accgttgccg cgttgcgcat tctactttgc
    20881 gagtctgctc cagaaaataa atcaaagtca gatcaatgcg ttgtgtttat ttaatgggga
    20941 tttacgatgg cgggactgta cagggctgta acggtaaagc cctcaccgaa gcgagggctt
    21001 gaaggagaag ggttatgatg cgacttgtca tcatactgat tgtactgtta ctcataagtt
    21061 tcagcgctta ttaacagtca gtcacagggg aggagcaatc cccccttacc cttactcact
    21121 aaattaggtt aaagaatcaa cgatgtcaat cagggcgatg caggtgtgtc gccctcacca
    21181 ctcccagact ttcgacggtg taaccaccgc aggcagtggg atatcccact cttcaacggg
    21241 gagtttttcc accaactgac aatcatgcgc ataacccacc ggttgcattt tatagtgctg
    21301 ccagttttgt aaggtccgat cataaaaacc accgcccatt cccaggcgct gaccgtactc
    21361 atcaaaggcg accagcggtg taatcaacac gtctaatcgg gaaaggggta gcacgtcacg
    21421 cacatccagt tttggctcat ggatcttcaa cttgttcatc accagttcgc tttgtggatg
    21481 gtaattcagg aacagcaaat taccggcact aaagggatgc aaaactggaa gatatacgcg
    21541 cttaccggcg cgccagagtt gttctatgag tggctgggtg tcgagttcgc catcaaaaga
    21601 gaggaataca gcgaccgtat gtgccatcac caccggggga taagtcatca tccgggtagc
    21661 ggcttgttga cccatttcct gctgttgttc cggcgttaac gcacgacgac gttgccgaat
    21721 cattttgcgg atttcttgtc gggataatgt cagtgggagt tctggtaatt gcgtcatggc
    21781 tggtgccaga taagaaggga atctccgaga tgccgccgca ggctgtaacc cttgaaccct
    21841 tggttcaagg tgaatgtgtc gtcgcagttt taaggcttct cggacggacc gagcatgctc
    21901 accaaccgcg gagcgccaca ttcttgtggt atgaaatatc ggctcagggg actggcccgc
    21961 ttgcgaacat ctcagagaaa ttttgtcttc acggttactc taccacagta aaccgaaaag
    22021 tgtcattcaa agttttggtt agttttttcg gtgatgcgac cttgttcaag taacgcttgt
    22081 tctatggtct gctgcagcat ccgaatacgc tgttccatac ttgccgcgta gtcacgagtc
    22141 ttcgcttttt cttgcgctaa ctcatagctg atattcagtg cggcaatgaa gaccaactgt
    22201 tcagtatttg tgactctagt gcgttctttc agatcttgca accgttggtt cagatcgtcc
    22261 gctgcctgat tcaacgcatc cctttggtca ggcgggcagt tcacacgcag tgaacggcca
    22321 aaaatttgga tatcgacggg ttgtgcagac atgccacctt cctgctgatt gactgcgctg
    22381 ccttcgtctt tagacccatg gtctgcgaag gggcgacact atagctaccc tgatgagaag
    22441 agacaagccc ttttctggtc caccagggcc caaagtggta gcatatcatg aatattcctc
    22501 cctttgacga cgaatgctta tgtctataca gaacgaaatg cctggttaca acgaaatgaa
    22561 ccagtatctg aaccaacaag ggacgggtct gacccctgct gagatgcatg gtttaatcag
    22621 tgggatgata tgtggcggta acgatgacag ctcatggctg ccgctacttc acgacctgac
    22681 gaacgaaggc atggctttcg gtcatgagct ggcacaggca ctgcgcaaaa tgcactctgc
    22741 caccagcgat gccctgcagg atgacggctt cctttttcag ctttatctac ctgatggcga
    22801 tgatgtcagc gttttcgatc gggctgatgc gctggctggt tgggtcaatc acttcctgct
    22861 tggtcttggc gttacgcaac cgaagctgga caaagtgacc ggcgaaaccg gtgaagccat
    22921 cgacgatctg cgtaacatcg cgcagttggg ttacgacgaa gacgaagatc aggaagagct
    22981 tgaaatgtcg cttgaagaga tcatcgagta cgtccgtgtt gccgcgctgt tatgccacga
    23041 cacctttact catccgcaac cgaccgcgcc agaagtacaa aaaccgactc tacactaaaa
    23101 acaaaaaacg taaggagagt gttatgagtg atatatcccg gcaagaattt cagcgtcgcc
    23161 gtcaggccct ggtagagcaa atgcaacccg gcagcgccgc gctgattttt gctgcaccag
    23221 aagtaacacg tagcgccgac agcgaatacc cctatcgtca gaacagtgac ttctggtact
    23281 tcaccggctt taacgaaccg gaagcggtgc tggtgctgat taaaagcgat gacactcata
    23341 accacagcgt tttgtttaac cgcgttcgcg acctgacggc agagatctgg tttggtcgtc
    23401 gcttaggcca ggatgccgcg ccagagaaac tgggcgttga ccgcgcactg gcattcagcg
    23461 aaatcaatca acaactttat caactactta atggtctgga tgtggtttac cacgcccagg
    23521 gcgaatatgc atatgctgat gaaatcgtga acagcgcgct tgagaaactg cgtaaaggtt
    23581 cgcggcaaaa tctcaccgca ccggcaacga tgatcgactg gcgtcctgtt gttcatgaaa
    23641 tgcgcctgtt caaatcacca gaagagattg ccgtacttcg ccgcgcgggt gaaatcaccg
    23701 cactggcgca tacacgggcg atggaaaaat gccgtccggg gatgttcgag taccatctgg
    23761 aaggcgaaat tcaccacgaa tttaaccgcc acggtgcgcg ttacccttcc tataacacca
    23821 ttgttggcag cggtgaaaac ggctgcattt tgcattacac cgaaaacgag tgtgaactgc
    23881 gtgacggtga cctggtgttg attgacgcgg gttgtgaata caaaggttac gctggcgaca
    23941 ttacccgcac cttcccggtc aacggcaaat tcactcaggc tcagcgtgaa atctatgaca
    24001 ttgtgctgga gtctcttgaa accagcctgc gcctgtatcg tccgggaact tccattcagg
    24061 aagtcactgg tgaagtggtg cgcatcatgg ttagcggtct ggtaaaactc ggcatcctga
    24121 aaggtgatgt tgatgaactg atcgctcaga acgcccatcg tcctttcttt atgcatggcc
    24181 ttagccactg gttaggactg gatgttcatg acgttggtgt ttatggtcag gatcgctcgc
    24241 gtattctgga accgggtatg gtactgaccg tagaaccagg actgtatatt gcgccggatg
    24301 cagaagtgcc agaacagtat cgcggtatcg gcattcgtat tgaagacgat attgtgatta
    24361 ccgaaaccgg taatgaaaac ctcaccgcca gcgtggtgaa aaagccggaa gaaatcgaag
    24421 cgttgatggc agctgcgaga aagcaatgag cgtaatcatc gtcggtggcg gcatggcggg
    24481 cgcgacgctg gcgctggcta tttcccggtt aagtcacggg gcgctgccag tacatttgat
    24541 tgaagcgacc gcgccagagt cacatgcgca tccgggcttt gatggacgag ctattgcgct
    24601 ggcggcgggt acctgtcagc aactggcgcg catcggcgtc tggcaatctc tggctgattg
    24661 cgcaactgcc atcaccaccg tgcatgttag cgatcgtggt cacgctggat ttgtcaccct
    24721 cgccgcagaa gattaccaac tggcggcgct ggggcaggtt gtcgaattgc acaatgtcgg
    24781 gcaacggctg tttgcattgc tgcgcaaagc acctggcgta acgctgcatt gccctgatcg
    24841 cgtggctaac gttgcccgta ctcagagcca cgttgaagtg acgctggaga gtggcgagac
    24901 gctgacgggc cgcgtgctgg tagccgctga tggcacccat tcagcgttag ccaccgcgtg
    24961 cggtgttgac tggcagcagg agccttacga acaactggct gtgattgcca acgttgcgac
    25021 ttccgttgcg catgaagggc gcgcttttga acgctttaca caatatggcc cgctggcgat
    25081 gttgccgatg tctgacggac gctgttcgct ggtctggtgt catccactgg aacggcgcga
    25141 agaggtgctg tcgtggagcg acgagaagtt ctgccgtgaa ctccagtcgg cctttggctg
    25201 gcgactgggg caaattaccc acgccggaaa acgtagtgct tatccgctgg cgttaacccg
    25261 cgccgccaaa cctattaccc atcgtaccgt gctggtgggg aatgcggcgc aaactctgca
    25321 tcccatcgcc gggcaaggat ttaacctcgg tatgcgtgat gtgatgagtc ttgcagaaac
    25381 cctgactcag gcgcaggaac gcagagaaga catgggtgat tacggcgtat tgtgccgtta
    25441 tcagcagcgt cgacagcgcg atcgcgaagc aaccatcggc gtcacggaca gccttgtaca
    25501 tctttttgcc aaccgttgga cgccgctggt tgtcgggcgc aacatcgggc tgatgacgat
    25561 ggaattattt accccggcac gcgatgtgct ggcgcagcgc actcttggtt gggtggcgcg
    25621 ttgaaggcgg ttttaaggaa taaacaatgc aaagtgttga tgtagccatt gttggcggcg
    25681 gcatggtcgg gctggcggtt gcctgcggct tacagggtat cggcttacgc gttgccgtac
    25741 tggagcagcg cgtaccggaa cctctggcgg cggatgcacc accacaactg cgcgtttcgg
    25801 ctatcaatgc cgccagcgaa aaattactca cccgtcttgg cgtctggcag gacattctct
    25861 ctcgtagggc ctgttgctat cacggcatgg aagtgtggga caaagacagc tttggtcaca
    25921 tctcgtttga cgatcaaagc atgggctata gccatcttgg gcatatcgtt gaaaactcag
    25981 tgattcacta cgcgctgtgg aacaaagcgc tgcagtcgtc agatatcact ctgttggccc
    26041 ccgcagaatt acagcaggtc gcctggggtg aaaatgaaac cttcctgacg ttgaaagatg
    26101 gcagcatgtt aacggcgcgt ctggtgattg gcgcggacgg cgctaattcc tggttgcgca
    26161 acaaagccga tattccgctg actttctggg attatcagca tcacgcgctg gtggcgacca
    26221 ttcgcacgga agaaccgcat gatgcggtgg cgcgacaggt ttttcatggc gaaggcattc
    26281 tggccttttt gccgcttagc gatccgcatc tttgctcgat tgtctggtca ctgtcgccag
    26341 aggaagcgca gcggatgcag caggcaggcg aagacgaatt taatcgcgcg ttaaatatcg
    26401 cttttgataa tcgcctgggc ttatgcaagg ttgaaagtga gcgcctggtg ttcccgctaa
    26461 cggggcgtta tgcgcgccag tttgccgcgc accgtctggc gctggtgggc gacgccgcgc
    26521 ataccattca cccgttggcg gggcaggggg taaatctcgg ctttatggat gctgcagagc
    26581 tgattgctga actgaaacgg ttgcatcgtc agggtaaaga catcgggcag tacatttacc
    26641 tgcgtcgcta tgagcgtagc cgcaagcaca gcgcggcgct gatgctggct ggtatgcagg
    26701 gattccgcga tctgttttcc ggggctaatc cggcgaaaaa attgctgcgt gatattggtc
    26761 tgaaactggc tgatacgctt cctggcgtta agccacaact tattcgccag gcaatgggat
    26821 taaacgattt gcctgaatgg ctgcgttaaa aatttctctt ctgctgttta tttgataccc
    26881 atcacacttt catctcccgg ttttttcgcc gggagatttt cctcatttga aataaactaa
    26941 tttcacctgc gatttcgcat tatattttct aatgccacga atttttgatt tagtgttttt
    27001 tgacattttt ttagttctta atgttgtctt attcaaatta actttctcat ctatttatct
    27061 ttatatagag atttgttcat tttttggttt tttattctgt tacgattttg gcgtttttta
    27121 accataagct aatgtgatga tcaattttac cttatggtta acagtctgtt tcggtggtaa
    27181 gttcaggcaa aagagaacga ttgcgttggg gaccgggagt ggctccgatg ctgggtttcg
    27241 tggtgataat ttcgccatag aaaagttgtc agccccgctt attcaatgag gacaagatgg
    27301 cacaacagac tcctttgtac gaacaacaca cgctttgcgg cgctcgcatg gtggatttcc
    27361 acggctggat gatgccgctg cattacggtt cgcaaatcga cgaacatcat gcggtacgta
    27421 ccgatgccgg aatgtttgat gtgtcacata tgaccatcgt cgatcttcgc ggcagccgca
    27481 cccgggagtt tctacgttat ctgctggcga acgatgtggc gaagctcacc aaaagcggca
    27541 aagcccttta ctcggggatg ttgaatgcct ctggcggtgt gatagatgat ctcatcgtct
    27601 actactttac tgaagatttc ttccgcctcg ttgttaactc cgccacccgc gaaaaagacc
    27661 tctcctggat tacccaacac gctgaacctt tcggcatcga aattactgtt cgtgatgacc
    27721 tttccatgat cgccgtacaa gggccgaatg cgcaggcaaa agctgccaca ctgtttaatg
    27781 acgcccagcg tcaggcggtg gaagggatga aaccgttctt tggcgtgcag gcgggcgatc
    27841 tgtttattgc caccaccggt tacaccggtg aagcgggcta cgaaattgcg ctgcccaatg
    27901 aaaaagcggc cgatttctgg cgcgcgctgg tggaagctgg cgttaagcca tgtggcctgg
    27961 gcgcgcgtga cacgctgcgt ctggaagcgg gcatgaattt gtatagccag gagatggacg
    28021 aaactatttc tcctttagcc gccaatatgg gctggactat cgcctgggaa ccggcagatc
    28081 gtgactttat cggtcgtgaa gctctggaag cgcagcgtga acatggcaca gaaaaactgg
    28141 ttggtctggt gatgaccgaa aaaggcgtgc tgcgtaatga actgccggta cgttttactg
    28201 atgcgcaggg caaccagcat gaaggcatta tcaccagcgg tactttctcc ccaacgctgg
    28261 gttatagcat tgcgctggcg cgcgtgccgg aaggtattgg tgaaacggcg attgtgcaaa
    28321 ttcgcaaccg tgaaatgccg gttaaagtga cgaaacctgt ttttgtgcgt aacggcaaag
    28381 ccgtcgcgtg atttactttt ttggagattg attgatgagc aacgtaccag cagaactgaa
    28441 atacagcaaa gaacacgaat ggttgcgtaa agaagccgac ggcacttaca ccgttggcat
    28501 caccgaacac gctcaggagc tgttaggcga tatggtgttt gttgacctgc cggaagtggg
    28561 cgcaacggtt agcgcgggcg atgactgcgc ggttgccgaa tcggtaaaag cggcgtcaga
    28621 tatttatgcg ccagtaagcg gtgaaatcgt ggcggtaaac gatgcactga gcgattctcc
    28681 ggaactggtc aacagcgaac cgtatgcagg cggctggatt ttcaaaatca aagccagcga
    28741 tgaaagcgaa ctggaatcac tgctggatgc aaccgcatac gaagcattgt tagaagacga
    28801 gtaacggctt tattcctctt ctgcgggaga ggatcagggt gaggaacatt tacgcctcac
    28861 cctcactctc ttcgtaagga gagaggttca caattcactg cacgtttcag gaaccatcgc
    28921 tcatgacaca gacgttaagc cagcttgaaa acagcggcgc ttttattgaa cgccatatcg
    28981 gaccggacgc cgcgcaacag caagaaatgc tgaatgccgt tggcgcacaa tcgttaaacg
    29041 cgctgaccgg ccagattgtg ccgaaagata ttcagcttgc gactccgccg caggttggcg
    29101 caccggcgac cgaatacgcc gcactggcag aactcaaggc tattgccagt cgcaataaac
    29161 gcttcacgtc ttacatcggc atgggttaca ccgccgtgca gctaccgccg gttatcctgc
    29221 gtaacatgct ggaaaatccg ggctggtata ccgcgtatac tccgtatcaa cctgaagtct
    29281 cccagggccg ccttgaagca ctgctcaact tccagcaggt aacgctggat ttaaccgggc
    29341 tggatatggc ctctgcttct cttcttgacg aagccaccgc tgccgccgaa gcaatggcga
    29401 tggcgaaacg cgtcagcaaa ctgaaaaatg ccaaccgctt ctttgttgct tctgacgttc
    29461 atccgcaaac gctggatgtg gtccgtactc gtgccgaaac ctttggtttt gaagtgattg
    29521 tcgatgatgc gcaaaaagtg ctcgaccatc aggacgtctt cggcgcgctg ttacagcagg
    29581 tcggcactac tggtgaaatt cacgactaca ctgcgcttat tagcgaactg aaatcacgca
    29641 aaattgtggt cagcgttgcc gccgatatta tggcgctggt gctgttaact gcgccaggta
    29701 aacagggcgc ggatattgtt tttggttcgg cgcaacgctt tggcgtaccg atgggctacg
    29761 gtggcccaca cgcggcattc tttgcggcga aagacgaata caaacgctca atgccgggcc
    29821 gtattatcgg tgtatcgaaa gatgcagctg gcaacaccgc tctgcgcatg gcgatgcaga
    29881 ctcgtgagca acatattcgt cgtgaaaaag cgaactccaa catttgtaca tcccaggtct
    29941 tgctggcaaa cattgccagt ctgtatgccg tttaccacgg tccggttggc ctgaaacgta
    30001 tcgctaaccg catccaccgt ctgaccgata tcctggcggc gggcctgcaa caaaaaggtc
    30061 tgaagctgcg ccatgcgcac tatttcgata ctttgtgtgt ggaagtggcc gacaaagcgg
    30121 gcgtgctggc gcgtgccgaa gcggctgaaa tcaacctgcg tagcgatatt ctgaacgcgg
    30181 ttgggatcac ccttgatgaa accactacgc gcgaaaacgt gatgcagctt ttcagcgtgc
    30241 tgttgggcga taaccacggg ctggacatcg acacactgga caaagacgtg gctcacgaca
    30301 gtcgctctat tcaggctgcg atgctgcgcg acgacgaaat cctcacccat ccggtgttta
    30361 atcgctacca cagcgaaacc gaaatgatgc gctatatgca ctcgctggag cgtaaagatc
    30421 tggcgctgaa tcaggcgatg atcccgctgg gttcctgcac catgaaactg aacgccgccg
    30481 ccgagatgat cccgatcacc tggccggaat ttgccgaact gcacccgttc tgcccgccgg
    30541 aacaggctga aggttatcag cagatgatcg cgcagctggc tgagtggctg gtgaaactga
    30601 ccggttacga cgccgtttgt atgcagccga actctggcgc acagggcgaa tacgcgggcc
    30661 tgctggcgat tcgtcattat catgaaagcc gcaacgaagg gcatcgcgat atctgcctga
    30721 tcccggcttc tgcgcacggc actaacccgg cttccgcgca tatggcagga atgcaggtgg
    30781 tggttgtggc gtgtgataaa aacggcaaca tcgatctgac tgatctgcgc gcgaaagccg
    30841 aacaggcggg cgataacctc tcctgtatca tggtgaccta tccttccacc cacggcgtgt
    30901 atgaagaaac gatccgtgaa gtgtgtgaag tcgtgcatca gttcggcggt caggtttacc
    30961 ttgatggcgc gaacatgaac gctcaggttg gcatcacctc gccgggcttt attggtgcgg
    31021 acgtttccca ccttaacctg cataaaactt tctgcattcc gcatggcggt ggtggtccgg
    31081 gtatgggacc gatcggtgtg aaagcgcatc tggctccgtt tgtaccgggt catagcgtgg
    31141 tgcaaatcga aggcatgtta acccgtcagg gcgcggtttc tgcggcaccg ttcggtagtg
    31201 cctctatcct gccaatcagc tggatgtaca tccgcatgat gggcgcagaa gggctgaaaa
    31261 aagcaagcca ggtggcaatt cttaacgcca actatattgc cagccgtctg caggatgcct
    31321 tcccggtgct gtataccggt cgcgacggtc gcgtggcgca cgaatgtatt ctcgatattc
    31381 gcccgctgaa agaagaaacc ggcatcagcg agctggatat tgccaagcgc ctgatcgact
    31441 acggtttcca cgcgccgacc atgtcgttcc cggtggcggg tacgctgatg gttgaaccga
    31501 ctgaatctga aagcaaagtg gaactggatc gctttatcga tgcgatgctg gctatccgcg
    31561 cagaaattga ccaggtgaaa gccggtgtct ggccgctgga agataacccg ctggtgaacg
    31621 cgccgcacat tcagagcgaa ctggtcgccg agtgggcgca tccgtacagt cgtgaagttg
    31681 cagtattccc ggcaggtgtg gcagacaaat actggccgac cgtgaaacgt ctggatgatg
    31741 tttacggcga ccgtaacctg ttctgctcct gcgtaccgat tagcgaatac cagtaattta
    31801 ctgattcgac tatcttctaa aggcgcttcg gcgccttttt cgcttgttaa taaagtgcgc
    31861 tttgtgtgtt tcggttgagg cgtaatataa aatattgcga gctcaatata ttaaaagagt
    31921 gccaggcctg atgagcggag cacatcaggc acatcggcta attacaaatt ctcgccgttg
    31981 ctggcaatca cctctttgta ccagttaaag ctcttcttac gtgaacgcga catatcgcca
    32041 gtaccgtcgt catgtttatt cacatagata aagccgtagc gtttgctgta ctgcccggta
    32101 gtgaacgaca cgcagtcgat gcagccccac ggtgtgtagc ccatcagatc cacgccatcg
    32161 taagtcaccg cttttttcat ctcttcaata tgggcgcgca ggtagtcaat gcggtaatcg
    32221 tcattgatgc tgccatcatc ttccactttg tcgtaagcgc caaaaccgtt ttcgacaata
    32281 aacagcggct tctggtaacg ctcatacagc tcgcaaagcg cgtaacgcag accaaccgga
    32341 tcaatctgcc agccccagtc agacgcttta acatacgggt tcggtacgct gccttcaaaa
    32401 ccagagatcg catcgccggt gccgccttcg gccttcaccg cgttggtcat gtaatagctg
    32461 aagccgagat aatcgcaggt gccttcgcgc agtacatcca gatcgccgtc ttccattttg
    32521 atgttaaatc cgcggcgttc ccactcgttc aacacatagg acgggtaata gccacgcaac
    32581 tgcacatcgg taaagacgta acgttcgcgc atcgacttct gggcaaacat cacatcgtcc
    32641 gggttacagg agtagggata gagcggcacc atcgccagca tacaaccgac tttcatctcc
    32701 gggttgatgc gatgcgcggc tttcaccgcc agggcgctgg cgacaaactg gtgatgcagc
    32761 acctgataca tggtctcttc cgggttttca tgttcggtat acaccacgcc ggagcagcag
    32821 taaccgaaca gcggtgcacg ccagttacgc tggttgttaa tttcgttgaa ggtcatccag
    32881 tatttgactt tgtgcttata gcgttcgaat accacttccg cgaaacggac aaagaaatca
    32941 accactttac ggttggtcca gctaccatat tgctgcacca gatgcagcgg catttcaaag
    33001 tgggagaggg tgatcaccgg ttcgatgttg tatttcagca gttcatcgat catatcatcg
    33061 tagaacttca gcccttcttc gtttggctga gcttcatcgc cttttggaaa aatgcgcgtc
    33121 caggcgatgg aagtacgaaa acatttgaag cccatttcgg caaatagctt gatgtcctcc
    33181 ttgtagtgac cataaaaatc aacggcttca tggtttggat agtatttgcc aggcacaact
    33241 tctttggtga tttcgcgcgg cacgccgtgt gcgccaccgg tcagaacgtc acaaatgctc
    33301 ggccctttgc cgcctttgtt ccagccgcct tcgacctgat gagcggcaac tgcgccgccc
    33361 cataagaaat ctttcggtaa ggtgagtttt ttcactatca taggctccag atgagtcaat
    33421 tcttacagga aatattacca tgcagccaaa cgacatcact ttttttcaac gtttccagga
    33481 tgacattctg gctgggcgta aaaccatcac catccgcgac gagtctgaat cgcacttcaa
    33541 aacgggtgat gtgcttcgtg tcggacgttt tgaagatgac ggttattttt gcacgattga
    33601 agtcaccgca acctcaaccg taacgctgga tacgctgacg gaaaaacatg cagagcagga
    33661 aaatatgacc ctgactgaac tgaaaaaggt cattgccgac atctatcccg accagacaca
    33721 gttttatgtg attgaattta aatgtcttta aattgaactc acgcctgtta gttgaaattt
    33781 cgcatttaat ttcatgattt ttcggagatt aatctcaaat cagaatttat tttagctaac
    33841 aggtgttcac tggaactatt ctcagttacg ctggagaggt atacatgtgc gtgtacgttt
    33901 ttccggagta agttatggtt cagaagcccc tcattaagca gggatattcg ctggcagagg
    33961 aaattgccaa cagcgtcagt cacggcattg ggctggtgtt tggtatcgtt gggctggtgt
    34021 tgctactggt tcaggcggtg gatctcaatg ccagcgccac ggcgataacc agttacagcc
    34081 tctatggcgg cagtatgatc ctgctgttcc tcgcttcgac gctctatcac gccattcctc
    34141 atcaacgggc aaaaatgtgg ctgaagaaat ttgaccactg cgctatttat ctgttgattg
    34201 ccggaaccta cacgccgttt ttgctggtgg ggctggattc tccgttagcg cgcgggttga
    34261 tgattgttat ctggagcctg gcattgctgg gtattctgtt taaactgacc atcgcgcacc
    34321 gattcaaaat tttatctctg gtgacctatc tggcgatggg ctggctgtcg ctggtggtaa
    34381 tttatgaaat ggcagttaag ctcgcggcgg gcagcgttac cttactggcg gtaggcggtg
    34441 tggtttattc gctcggggtg attttctacg tctgcaagcg cattccatac aatcatgcca
    34501 tctggcacgg ctttgtgctc ggcggtagtg tgtgccactt tctggcgatc tatttgtata
    34561 ttgggcaggt gtaattggcg tgcctttgta ggcctgaaaa gacacaccag tgtcgcatca
    34621 ggcgataaat gcctgatgcg atacagagat ttactcttcc agcgaatatg gcagtggctc
    34681 aatacgcaat gtattcgcat cgtcacgcac gcggaagatg ctgtccggtt ccatatcgtt
    34741 attcatgaca acctgcacca caacctgacc atcttccagt ttgaccgcag ccagcaccgt
    34801 accggtacga cgccagttct cgcccatttt cagctctaag tcttcaccag cttccggcag
    34861 tcggctggcg ctacccgtca gcaaccagag cgcgcgtttg ttggcaccac ggaattttgc
    34921 tcgcgccacc atctcttgtc cggtatagca gcctttctta aagctgatgc cgcccagcgc
    34981 ctggaggttg gtcgcctggg ggataaactg cccgctgttg gcggcatcaa tcaccggaaa
    35041 acccgcttca atgtttaatg ccagccactg ttggctattg ttcagttccg cttcaccgcg
    35101 cagtttatcg gtcagcatat tggcagtagc ttcatcggtt acgatcagga aacgttctgc
    35161 cgggtgttca aaccatagca acgtggtcgc gccttctttg actacctgtt tttctctcga
    35221 aggtagttca ctaaagagat ttgccaacgc ggcgcgcgcc tgaaaaccgg caacaccaag
    35281 cagcacacgc tcgtcgtctg gcgcgatggt cactttagag aacaccgcat attttttcag
    35341 ttcagtcagc tgcggttcac gcacgctgcg ccgttcaatc catgcaaagc catcgccgtc
    35401 gcggaacaga cgtaaattgc tccacatttt acctttggcg tcgcaatggg cggcgagcag
    35461 gtgctgatct tcagtcatct ggctgacatc tgctgtcacc tggccttgca tatatttttc
    35521 gctgtccgcg ccagtaatag tggcaagcgc ccagtcatca agcgtcatca gcgtcagtgg
    35581 caaacgggca gaagccgtag gctgacgggg aggaaaaggt gtaaaagcca taatagagtc
    35641 ctgagttgct taacggcgta ttaatcacta atggtaaaag agctatgagg caatgcaagt
    35701 gctttcactg cgcgatctgt gccttctgcg aggcgacttc caaaaaaatg aagcccttga
    35761 attgttgtga attaatttcg cgatcgcgat tgtgtttact gatattcccg taaaagaagt
    35821 cgaaaaacac gttacaatag ccgggtactg gattttcgtg agacaggaag aagatggaca
    35881 ttaacaacaa agcccgcatt cattgggcat gccgccgtgg tatgcgcgaa ctcgatattt
    35941 caatcatgcc gtttttcgaa catgagtacg acagcttaag cgatgacgaa aaacgcacct
    36001 ttattcgtct gctggaatgt gacgatccgg acctgtttaa ctggctgatg aatcacggta
    36061 aaccagccga tgcagaactg gaaatgatgg tccgactcat ccagacacgg aaccgggaac
    36121 gtggtcctgt ggcaatctga tttgcgcgtc tcctggcgcg cacagtggct ttccttgctg
    36181 attcatgggc tggttgccgc tgttatttta ctcattccct ggccgctcag ttacaccccg
    36241 ttatggatgg tgttactttc gctggtggtg tttgattgcg ttcgcagcca gcggcgcatt
    36301 aatgctcgcc agggggaaat tcgcttgttg atggacgggc gtttgcgttg gcaagggcag
    36361 gagtggagca tcgtcaaagc gccgtggatg attaagagcg gcatgatgct gcgtttacgt
    36421 tctgacagcg gaaaacggca acatttatgg ctggcagctg acagcatgga cgaagccgaa
    36481 tggcgggatt tacggcggat attgttgcaa caagagacgc aaagataaag atgacggcaa
    36541 agcatatgtg ctaagccgtc agtgacatca ggcgtaatgc tctgccattt cgttgaggat
    36601 ttgctcgcac cagctttgaa tacgctcgtc gctaaggtca tactggttag tttcatccag
    36661 cgccagaccc acgaacagtt gcccgtcagc aatcaccggt ttcggactgg taaattcata
    36721 tccttccgtt ggccagtagc cgacgaactt cacgcctttg gtcgagagtt tgtcatgcag
    36781 cataccgagc gcatcgagga accactcgcc gtatcccagt tgatcgccaa gcccatacag
    36841 cgcaacaatt ttaccttcga ggttcaggtc gtcgagctga tcccagacgg cttcccagtc
    36901 ttcctggatt tcgccaaaat cccaggtcgg gatacccaga atgagcacat cgtactgctc
    36961 cattaatttc ggggagtcgt ccttgaggtt atgtaaggtc accagttctg ggccgataat
    37021 atcgcggatt ttttctgccg ccatttcggt gtaacaggtg ctggaaccgt aaaaaagacc
    37081 catattcata acgtaagctt ctcgattctg ctgtggcttt tgcggctagt gtatcagaat
    37141 cgctttagtc acggcacaat aaggcataat ggatcaaatg agtgaccata aggggcgcaa
    37201 gtgaaacagg atctggcacg catcgagcag tttcttgatg ctctgtggct ggaaaaaaat
    37261 ctggctgaaa atacgttgaa cgcttaccgt cgcgatctgt caatgatggt ggagtggttg
    37321 catcaccgcg ggttgacgct ggcgacggcg caaagtgacg atttgcaggc attactggca
    37381 gaacggctgg agggcgggta taaagccacc agctcagcgc gtttgctgag tgcagtgcgc
    37441 cgattgttcc agtatcttta tcgcgaaaag tttcgtgaag atgatcccag tgcgcatctc
    37501 gcttcaccga aattgcccca gcgtttgcca aaagatttaa gcgaagcgca ggtcgaacgt
    37561 ttattacagg caccattaat tgatcagcca ctggagctac gcgataaagc catgcttgaa
    37621 gtgttgtatg ctaccggact gcgtgtctct gaactggtcg ggctgacaat gagtgatatc
    37681 agcctgcgtc agggcgtggt gcgggtcatt ggtaaaggca acaaagagcg cctggtgccg
    37741 ttaggtgaag aggcggttta ctggctggaa acctatctgg aacatgggcg tccgtggctg
    37801 ttgaatggtg tgtcaattga cgtgttgttt cccagccagc gtgcgcagca gatgacgcga
    37861 cagaccttct ggcaccgcat taaacattat gccgtgctgg cgggtatcga cagcgaaaag
    37921 ctgtcaccgc atgtgttgcg tcacgctttt gccactcact tattaaatca tggtgcggat
    37981 ttacgcgtgg tgcagatgct actgggccac agcgatctct ccaccacgca aatttatacg
    38041 catgtcgcta ccgagcgtct gcgacaactt catcaacagc atcacccgcg ggcgtgatgt
    38101 ctgaaaagaa caggaaaatt tatgaagaaa ggttttatgt tatttacctt gttggcagcg
    38161 ttttcaggct ttgttcaggc tgatgacgcg gcaattcaac aaacgttagc caaaatgggc
    38221 atcaaaagca gcgatattca gcccgcgccc gtagctggca tgaagacagt tctgactaac
    38281 agcggtgtgt tgtacatcac cgatgatggt aaacatatca ttcaggggcc aatgtatgac
    38341 gttagtggca cggctccggt caatgtcacc aataagatgc tgttaaagca gttgaatgcg
    38401 cttgaaaaag agatgatcgt ttataaagcg ccgcaggaaa aacacgtcat caccgtgttt
    38461 actgatatta cctgtggtta ctgccacaaa ctgcatgagc aaatggcaga ctataacgcg
    38521 ctggggatca ccgtgcgtta tcttgctttc ccgcgccagg ggctggacag cgatgcagag
    38581 aaagaaatga aagctatctg gtgtgcgaaa gataaaaaca aagcgtttga tgatgtgatg
    38641 gcaggtaaaa gcgtcgcacc agccagctgc gacgtagata ttgccgacca ttacgcactt
    38701 ggcgtccagc ttggcgttag cggtactccg gcagttgtgc tgagcaatgg cacacttgtt
    38761 ccgggttacc agccgccgaa agacatgaaa gaattcctcg acgaacacca aaaaatgacc
    38821 agcggtaaat aattcgcgtg aaacaacaga tacaacttcg tcgccgtgaa gtcgatgaaa
    38881 cggccgactt gcccgctgaa ttgcctccct tgctgcgccg tttatatgcc agccggggtg
    38941 tgcgtagcgc gcaggaactg gaacgcagtg ttaaaggcat gctgccctgg cagcaactga
    39001 gcggcgtcga aaaggccgtt gagatccttt acaacgcttt tcgcgaagga acgcggatta
    39061 ttgtggtcgg tgatttcgac gccgacggtg ccaccagcac ggctctaagc gtgctggcga
    39121 tgcgctcgct tggttgcagc aatatcgact acctggttcc aaaccgtttc gaagacggtt
    39181 acggcttaag cccggaagta gtcgatcagg ctcatgcccg tggcgcgcag ttaattgtca
    39241 cggtggataa cggtatttcc tcccatgcgg gggttgaaca cgctcgttcg ttgggcattc
    39301 cggttattgt taccgatcac catttgccag gcgacacatt acccgcagcg gaagcgatca
    39361 ttaaccctaa cttgcgcgac tgtaatttcc cgtcgaaatc actggcgggc gttggcgtgg
    39421 cgttttatct gatgctggcg ctgcgcacct ttttgcgcga tcagggctgg tttgatgagc
    39481 gcggcatcgc aattcctaat ctggcagaac tgctggatct ggtagcgttg gggacagtgg
    39541 cggacgtcgt gccgctggac gctaataatc gcattctgac ctggcagggg atgagtcgca
    39601 tccgtgccgg aaagtgccgt ccggggatta aagcgttgct ggaagtggcc aatcgcgatc
    39661 cacaaaaact cgccgccagc gatttaggtt ttgcgctggg gccacgtctc aatgctgccg
    39721 ggcgactgga cgatatgtcc gttggcgtgg cgctgttgct gtgcgacaac atcggcgaag
    39781 cgcgcgtgct ggcaaatgaa ctcgatgcgc taaaccagac gcgaaaagag atcgaacagg
    39841 gaatgcaagt tgaagccctg accttgtgcg agaaactgga gcgcagccgc gacacgctac
    39901 ccggcgggct ggcaatgtat caccccgaat ggcatcaggg cgttgtcggt attctggctt
    39961 cgcgcatcaa agagcgtttt catcgtccgg ttatcgcctt tgcgccagct ggtgatggta
    40021 cgctgaaagg ttccggtcgc tccattcagg ggctgcatat gcgtgatgcg ctggagcggc
    40081 tagacacgct ttaccccggc atgatgctca agtttggcgg tcatgcgatg gcggcgggtt
    40141 tgtcgctgga agaggagaaa ttcgaactct ttcaacaacg gtttggcgaa ctggttactg
    40201 agtggctgga cccttcgcta ttgcaaggcg aagtggtgtc ggacggtccg ttaagtccgg
    40261 ccgaaatgac catggaagtg gcgcagctgt tgcgcgatgc tggcccgtgg gggcagatgt
    40321 tcccggagcc gctgtttgat ggtcatttcc gtctgctgca acagcggctg gtgggcgaac
    40381 gtcatttgaa agtcatggtc gaaccggtcg gcggcggtcc actgctggac gggattgctt
    40441 ttaatgtcga taccgccctc tggccggata acggcgtgcg cgaagtgcaa ctggcttaca
    40501 agctcgatat caacgagttt cgcggcaacc gcagcctgca aattatcatc gacaatatct
    40561 ggccaattta gcgtcatctt ctctataaaa aagagcgtgg attgggtaca atcccgctct
    40621 tatcaccgca ttttgactag ctcaataaaa gaaatcagac catgtttgaa attaatccgg
    40681 taaataatcg cattcaggac ctcacggaac gctccgacgt tcttaggggg tatctttgac
    40741 tacgacgcca agaaagagcg tctggaagaa gtaaacgccg agctggaaca gccggatgtc
    40801 tggaacgaac ccgaacgcgc acaggcgctg ggtaaagagc gttcctccct cgaagccgtt
    40861 gtcgacaccc tcgaccaaat gaaacagggg ctggaagatg tttctggtct gctggaactg
    40921 gctgtagaag ctgacgacga agaaaccttt aacgaagccg ttgctgaact cgacgccctg
    40981 gaagaaaaac tggcgcagct tgagttccgc cgtatgttct ctggcgaata tgacagcgcc
    41041 gactgctacc tcgatattca ggcggggtct ggcggtacgg aagcacagga ctgggcgagc
    41101 atgcttgagc gtatgtatct gcgctgggct gaatcgcgtg gtttcaaaac tgaaatcatc
    41161 gaagagtcgg aaggtgaagt ggcgggtatt aaatccgtga cgatcaaaat ctccggcgat
    41221 tacgcttatg gctggctgcg tacagaaacc ggcgttcacc gcctggtgcg taagagcccg
    41281 tttgactccg gcggtcgtcg ccacacgtcg ttcagctccg cgtttgttta cccggaagtt
    41341 gatgatgata tcgatatcga aatcaatcca gcggatctgc gcatcgacgt ttatcgcgca
    41401 tccggcgcgg gcggtcagca cgttaaccgt accgaatctg cggtgcgtat tactcacatc
    41461 ccaaccggga tcgtgaccca atgccagaac gaccgttccc agcacaagaa caaagaccag
    41521 gccatgaagc agatgaaagc gaagctttat gaactggaga tgcagaagaa aaatgccgag
    41581 aaacaggcga tggaagataa caaatccgac atcggctggg gcagccagat tcgttcttat
    41641 gtccttgatg actcccgcat taaagatctg cgtaccgggg tagaaactcg caacacgcag
    41701 gccgtgctgg acggcagcct ggatcaattt atcgaagcaa gtttgaaagc agggttatga
    41761 ggaaccaaca tgtctgaaca acacgcacag ggcgctgacg cggtagtcga tcttaacaat
    41821 gaactgaaaa cgcgtcgtga gaagctggcg aacctgcgtg agcaggggat tgccttcccg
    41881 aacgatttcc gtcgcgatca tacctctgac caattgcacg cagaattcga cggtaaagag
    41941 aacgaagaac tggaagcgct gaacatcgaa gtcgccgttg ctggccgcat gatgacccgt
    42001 cgtattatgg gtaaagcgtc tttcgttacc ctgcaggacg ttggcggtcg cattcagttg
    42061 tacgttgccc gtgacgatct gccggaaggc gtttacaacg agcagttcaa aaaatgggac
    42121 ctcggcgata tcctcggcgc gaaaggtaaa ctgttcaaaa ccaaaaccgg cgaactgtct
    42181 atccactgta ccgagctgcg tctgctgacc aaagcactgc gtccgctgcc ggataaattc
    42241 cacggcttgc aggatcagga agcgcgctat cgtcagcgtt atttggatct catctctaac
    42301 gatgaatccc gcaacacctt taaagtgcgc tcgcagatcc tctctggtat tcgccagttc
    42361 atggtgaacc gcggctttat ggaagttgaa acgccgatga tgcaggtgat ccctggcggt
    42421 gccgctgcgc gtccgtttat cactcaccat aacgcgctgg atctcgacat gtacctgcgt
    42481 atcgcgccgg aactgtacct caagcgtctg gtggtcggtg gcttcgagcg tgtattcgaa
    42541 atcaaccgta acttccgtaa cgaaggtatt tccgtacgtc ataacccaga gttcaccatg
    42601 atggaactct atatggctta cgcagattac aaagatctga tcgagctgac cgaatcgctg
    42661 ttccgtactc tggcacagga tattctcggt aagacggaag tgacctacgg cgacgtaacg
    42721 ctggacttcg gtaaaccgtt cgaaaaactg accatgcgtg aagcgatcaa gaaatatcgc
    42781 ccggaaaccg acatggcgga tctggacaac ttcgactctg cgaaagcgat tgctgaatct
    42841 atcggcatcc acgttgaaaa gagctggggt ctgggccgta tcgttaccga gatcttcgaa
    42901 gaagtggcag aagctcatct gatccagccg accttcatta ctgaatatcc ggcagaagtt
    42961 tctcctctgg cgcgtcgtaa cgacgttaac ccggaaatca cagaccgctt tgagttcttc
    43021 attggtgggc gtgaaatcgg taacggcttt agcgaactga atgacgcgga agatcaggcg
    43081 cagcgcttcc tggatcaggt tgccgcgaaa gatgcaggtg acgacgaagc gatgttctac
    43141 gacgaagatt atgtcaccgc actggaacat ggcttaccgc cgacagccgg tctgggaatt
    43201 ggtatcgacc gtatggtgat gctgttcacc aacagccata ccatccgcga cgttattctg
    43261 ttcccggcga tgcgtccggt aaaataagca ttacgttatg ctcacaaccc cggcaaccgt
    43321 cggggttttt tatttaagtt gggtaaatgc agataatcgt tttctggctt cgcgatttgt
    43381 cgcctgcatc accatccacg gactgaacgc ccacggcgta gcatcaatac cgcgtaaaac
    43441 cgctgctaaa tcacaccatt gataatccat cacttcatca tcgttgatct gtaacgcact
    43501 ggtcgtgcgt gcggcaaata ccggacacac ttcattttcc acaatgccat tcggatcggt
    43561 ggcgcggtag cgaaagtcag gatagataga ttcaggaggc gtaatttcta cgccaagctc
    43621 ataacggcaa cggcggatca ccgcctcttc gttgctttct cccagttgtg ggtgcccaca
    43681 aaccgagtta gtccacacgc caggccatgc ttttttgcta agggcgcggc gggtaactaa
    43741 taattgccct ttggcattaa acagccaact ggaaaacgcg agatgtaaga gggtgtctgc
    43801 cgtgtgtgcg gcatactttt ccagcgtacc cgtgggaact ccctgtgcat tcaataaaat
    43861 gacgtgttcc gtttgcataa tttctcacat gtaattctga tcactcatca ttatagataa
    43921 tagaagagtg aaatgattaa ccaagtgagc gtttatcgac aaccgcccgt tttgagcgga
    43981 tgtcgacagg taaaaaccat ttaaaaaagc agcatcatat atttgaagcg actttcaatg
    44041 gccattgcaa cacaatcaga aatgacagga atagagagag taaaaagcaa cagcagactg
    44101 aatatagcgc gcattaaaag attcatatta ttctccatgc tcatttttct tcagcttgcg
    44161 catcagaagc cacatacgta ctgtcctgac ttttaaatct ttatcagaga cggattcctt
    44221 caccaggtca gcatgatgct ggtagccatt aaagaagacg ttcagcacaa cggcgctaag
    44281 ggttgcgagc ataatgccgc tatgcagcaa cggttgcagt acggccggta acttagaaaa
    44341 gaaatcgtga gagagcgttg gcgtcatgcc aacaccgaga ctgatcgcca caatatagag
    44401 gttgtaacgg ttagtggtgt agttacagcg cgacagaatt cgaatccctg tcgccagtac
    44461 catgccgaac atcaccagcc ccgcgccgcc cagcacaaat tgcggaatgg aggcgaccag
    44521 caccgccatt ttgggcacca tgccaaacag gattaatata attcccgaag aaatacacac
    44581 ccagcggctg tggacgcgcg tcacgctgac cagcccgacg ttctgcgaaa atgacgtatg
    44641 tgggaagctg ttaaacgtgc ctccaatcat tgtcccgacc ccatctacgc gtaacccacg
    44701 aataatgtcc tgggaagaga gtttgcgacc gactatttca cccagtgcca ggaacatccc
    44761 cattgactca ataaatacga tgatgagtac tgcggtcatg gtcaaaatgg aaacgggatc
    44821 gaaaatcggc ataccaaatg acatcggcgt gacaatcgca aaccatgaag catcatgtag
    44881 cccggataaa ttgacttcat tcatcatcca cgaaagtaaa aagccaaata caatccccag
    44941 taatacggcg acgttggaca taaatccttt cgcatagcga gtaatgagca agataaaaat
    45001 taagacggca aaggagatac ctaaataaac gggattacca tattgcggat tccctttacc
    45061 tccggcggcc cagtcaatac ccacctgaat gatgctaagc ccgatagaag taatcaccac
    45121 accggtaacc agtggcggga ataaaggcat caagcgaccg ataagtggcg ctaataatgt
    45181 ggtgataaaa cccgcggcga tagtggcacc gaatatcccc agcaggccga tatccgggtt
    45241 cataccaatg gctatcattg gtgttacagc ggcgaaggtc accgacataa tcaccggcag
    45301 gcggatcccc ataaagcggc cgataccgat acattgcaat aatgtgacga tcccgcagca
    45361 aaagagatcc gagctaatga gcatcgcaat agcttctttt gataggccga gtcggtcacc
    45421 aatcataaga ggaacagcga ctgcacctgc gtacatgaca aggacgtgtt gcagaccgag
    45481 tatgatcagc tttcctggtg ataatatgcg gtcaacctca tcagttgggc ggtcttgccc
    45541 agaagatgag ggaagttggg aatctatggc gctcatcagt gtttctcctt tcgcccgcca
    45601 gagtaaaaag caaccgttta tgttcatgaa actgtctaaa cacatgctcg tggagtgcca
    45661 gtttttgcga ggaaggggag ttagagacag gaattgcgga ggcaatcaca taatcgtggt
    45721 taaaccacga aaatgagaag agaacctgac aatggaatta tctgtaattt tctcatattg
    45781 atatttcatt aatcattctg gcatgtcgca ctctcgcatt taatcgtttt tatctggata
    45841 gcattctttt gatcggcaat gttgaaataa tgaaagcggc atagattgag gggcaataat
    45901 ttgaactcag ttgcaaaatt ataattaaaa cggtcatgct acctaatata tgcccgttta
    45961 ttgcatcgcg aaatttgaga agacataact cattaaatga aagggatgca aatgaataag
    46021 tttatcgctg ctgaagctgc ggaatgtata ggctgccatg cttgtgaaat tgcctgtgcg
    46081 gtggcacata atcaagaaaa ctggccgctg agtcacagtg actttcgacc gcgtatccac
    46141 gttgtaggga aaggccaggc tgcgaatccg gtggcctgcc atcactgcaa caatgcccct
    46201 tgcgttacgg cttgtccggt taatgctctg actttccagt ccgatagcgt acaactggac
    46261 gagcaaaaat gtattggttg taaaagatgc gcaatcgctt gcccctttgg cgtcgttgag
    46321 atggtcgata cgattgcaca gaaatgcgac ctttgtaacc agcgcagttc cggcacgcaa
    46381 gcctgtattg acgtctgccc aacacaggcg ttacggctga tggacgataa agggttgcag
    46441 cagataaagg tggcccgcca gcgcaaaacg gcagcaggaa aagcgtcatc agacgctcag
    46501 ccatctcgca gtgcagcgtt gctccccgtt aactcgcgta aaggcgcaga taaaatttca
    46561 gcgagtgaac ggaaaaccca ctttggcgaa atctattgcg ggctggatcc acaacaagcg
    46621 acttatgaga gtgaccgctg tgtttattgt gccgaaaaag ctaactgcaa ctggcattgt
    46681 ccgctgcata acgctattcc ggattacatc cgtctggtac aggaaggaaa gattattgaa
    46741 gcggcagaac tttgccacca gaccagttcc ttacccgaaa tctgcggcag ggtatgtcca
    46801 caggaccgtc tttgtgaagg tgcatgtact ttgaaagatc actctggcgc agtctctatc
    46861 ggtaatctgg aacgctacat caccgatact gcgctggcga tgggctggcg tcccgatgtc
    46921 agcaaagttg ttccccgtag cgaaaaagtg gcggtgattg gcgctggacc tgcaggatta
    46981 gggtgtgctg atattctggc gcgcgcggga gttcaggttg atgtctttga tcgccatcca
    47041 gaaattggcg gtatgctgac ttttggcatt cctcctttca aactcgataa aacggtatta
    47101 agccagcggc gagagatatt caccgcaatg ggaatcgatt tccatctcaa ctgtgaaatt
    47161 ggccgcgata tctctttcaa cgaattaacg gcggaatatg atgctgtttt cctcggcgtg
    47221 gggacttacg ggatgatgcg agcagatctg ccgcatgaag atgcgcctgg tgtcattcag
    47281 gcgttaccgt tcctgaccgc ccatacccgc cagctcatgg gattgccgga gtctgcagag
    47341 tatccgctga cggatgtgga aggtaagcga gttgtggtat tgggcggtgg cgatacgaca
    47401 atggattgtt tgcggacttc catccgcctt aatgctgcca gcgtgacctg tgcgtatcgt
    47461 cgtgatgaag tcagtatgcc gggttcgcgc aaagaggtgg tcaatgcccg cgaggaaggt
    47521 gttgagtttc agttcaatgt tcagccgcaa tatatcgctt gtgatgaaga tggacgctta
    47581 acggcggtgg gcctgattcg taccgctatg ggtgagccgg ggccggatgg ccgccgtcgt
    47641 cctcgtccgg ttgcgggttc agagtttgaa ttgcccgccg atgttctcat tatggccttt
    47701 ggtttccagg cacataccat gccgtggttg cagggcagcg gaattaaact cgataaatgg
    47761 ggcctgattc aaaccggcga cgtcgggtat ttacccaccc agacgcatct gaaaaaagtc
    47821 tttgctggtg gtgatgcagt tcatggcgca gatctggttg tcactgcaat ggccgcagga
    47881 aggcaggcgg cgcgcgatat gttaactctg tttgatacga aggcatcgtg atgaaatcgt
    47941 taattatcgt taatccggct gactgtattg gctgccgtac ttgtgaagtg gcctgtgtgg
    48001 tcgctcaccc ttcagaacag gagttgaatg ccgatatctt tttaccccgg ctgaaggtac
    48061 aacggctgga tagcatcagt gcgccggtga tgtgccatca gtgtgaaaac gccccttgtg
    48121 ttggcgcttg tcccgtgggg gcgctgacga tgggcgagca ggtggtgcag gcaaattctg
    48181 cccgttgtat tggctgtcag agttgcgtca gtgcgtgccc gtttggaatg atcaccattc
    48241 agtcattgcc gggggatacc cggcaacaaa tcgtgaaatg cgatctctgt gaacagcggg
    48301 aagaggggcc agcctgcgtt gaatcttgcc cgacacaggc gctgcagttg ctgactgaaa
    48361 gagaactcag gcgagtccgc cagcagcgta ttgttgccag cagtgagaat cctctgtgaa
    48421 agaaatctcc gcgctgaaag gcgcggagaa tctattagat tgcccaacca cccgcgtaaa
    48481 acgcgaccag tgcggcagta atgatcaccg taccaatatt cagcttttgc cattcgcgcg
    48541 caaagacgcg acccaccacc agtgttacaa agcccagcat aataccggta acgatattac
    48601 aagtcagaac gatgaacacg gcacacacca ggccagccat tgcgtcaata aaatcgttga
    48661 aatccagctt cgagacgttg cttaacatca gcaaacccac gtacatcagt gcgggagctg
    48721 tggcataacc ggggatcaga aacgataacg gtgataaaaa cagaatcatc aggaacaaca
    48781 caccaaccac ggttgcggtt aaaccggttt tccccccggc ggcggttcct gccgctgatt
    48841 cgatataaac cgccgcgggc gctgcgccca ccaggccgga gaatattgaa cttactgagt
    48901 cactggtcag ggctttgccg ccgttgatga tctggttgtc tttatccaac aaattcgcct
    48961 gaccggcgac ggcacggatg gtgccagtgg cgtcgaacac tgcggtcatc accaatgcca
    49021 gtacactcgg aagtacagtt ggctggagtg cgcccataat atcgaggctg aaaatcagag
    49081 acttaccatc ttcgccagtc aggcttggca tcgccaccag accgtggtac ttcaccgctg
    49141 ggtcaaagat taagccgatg atcgaaattg caataatcac caacaagatc ccgccgggta
    49201 cgcggcactt ttccagaccg aagatgaccg ccagccccag taagctcatc atcaccggga
    49261 aggaggtaaa ggcaccgagc gccactggca agccttcaat cgggtttttt ataaccattc
    49321 ccacaccgtt agcagcaatc agcagcagaa acaggccgat accgatacct gtaccgtgag
    49381 cgatccccat cggcaaatta cgtaagatcc aggtacgcac accggttacg gaaatagcgg
    49441 tgaagatgac gcccatcaga aagactgcgc ccagtgcgac aggaacgcta atttgttgcc
    49501 cgagtaccag actgaatgcg gtaaacgccg tcaaggaaat agcgcaacca atcgccattg
    49561 gcaaattggc ccataacccc atcagcaacg agccgaagcc cgcgaccaga caggtggcaa
    49621 caaacacagc tgcgggagga aaacctgctt tgcccagcat tcccggaacg acgataacgg
    49681 aataaaccat ggccagaaag gtcgttaagc cagccagtac ttcctgacga acggtactgc
    49741 cacgagcggt aattttaaaa taattatcca gcgcgccctg tggctttggt gcgtccggtg
    49801 tttgtaggat gtctccagac ataatgatgt cctcacgagg atgttaaagt tttattatta
    49861 gttgcgttcg tacaccagac gaccatcaac gtaggtgcgg tagatcgaac ggtcatcgcc
    49921 caacgtcatc atcacgaaca atttgtcgac taaagaaaca gagttgtcat agcgcagctg
    49981 ctgtagcgga gtggcagtgg gttccatcac cacgaaatca gcctctttgc caggtaaaaa
    50041 gttgccaatc aaatcgtcaa ggcccagaga tttcgctccg ccgagcgtgg ccaggtaaaa
    50101 cgcttcatat gccgagaggc gatagccttg taattgcaat accttgtagg cttcgttcag
    50161 cgtttgcagc atgttaaaag ttgttccggc accgatatcc gttcccatgc ccactttgac
    50221 tttcttctgc catgcttttt tcaagttgaa taagccgctg ccgaggtaaa ggttggaggt
    50281 cggacagaaa gcaatgctgg atttggtttc gctgagacga tcccactctt tttcttcgag
    50341 atggacgcag tgagcaaaga cgcagttttt accggtcagg ccgtactgat ggtaaacatc
    50401 aagataacca tcatggtcag gataaagcga tttcacccag gcaatttcat ctttgttttc
    50461 acagagatgg gtatgtaccc atgtatccgg atattcttct ttcaggcgtt gcgccatcgc
    50521 catctgttca ggagatgagg taggggcgaa gcgtggcgta atcgcataca acagacgacc
    50581 atttttgtgc cagcgttcaa tcagttcttt gctttggtga tagctgcttt cggcagtgtc
    50641 gagcagataa tccggtgcgt ttcggtccat catcacttta ccggcaatca tacgcatatt
    50701 gatatgactg gcggcttcaa acagcgcatc aacggattgt ggatgaacag tgccaaacac
    50761 cagcgccgtg gtggttccgt tacgtaaaag ctgcttgatg aagaacgccg acatctcgcg
    50821 ggcgtactct aaatcctcat aacgacgttc agtagggaag gtgtgtttat tcaaccactc
    50881 cagcaattgt tcaccatagg cccccaccat ttcactttgc ggataatgga tatgtgtatc
    50941 gataaagccc ggtactatca gtttgccgcg atagtcgcgc acgcgaatgg tgtcaggaat
    51001 ttgatgcttt ccgtcttccc attcgccaaa ccattccact tttccctgtt taatgagtaa
    51061 taaaccatcc tcaataaacc gcagcgcaga ggcaatttct tccgggttat cgaccgtacg
    51121 ggtgacatca ataaaactgc ctcgtaccgc ttttaacgtg tgttctcctg acatcattga
    51181 ctcctttact gttaatccat ctcttccgct gaggtaatac caggcagaac gttttcctgt
    51241 cggtagccac cagggaggat aatatttaat aaaatcgcag ttaacccgcc agcacaaata
    51301 gggttttcaa ctaatacata aatagaggct ggcaatattt taaaaatttc gggatcgtag
    51361 gagacgccaa gtcctaaacc taaagaagtg gcgacaataa gtgtttctcg gcgctttaaa
    51421 ccgttggtga tgatgatgcg aatccctgcg atggcaatca tggaaaacat caacgtcatt
    51481 gcgcctccca gaactgccga gggaatggtc gtaaaaaagc cgccaatcat cggaaataag
    51541 ccgaggataa ccagcattac cgcgatggtt cgcccgacat aacgtgaagc gacgccagtc
    51601 atctgaataa ccccattatt ttgcgcaaac gtggttaatg gtaatgaacc gacagcggag
    51661 gcgataacag aaaccagacc atctgccagc acgccgcctt tcagtcggga ctgatactct
    51721 tccccctgaa tggggcggcg ggaaaccatt gctgtggcgg tgatatcgcc gacagcttcc
    51781 agcacgctaa gcagataaat cgtgccaacc accaggaact gatggaagct aaaactaaag
    51841 ccgtatttga acggatgcgg gatggtgatt aacggcaaat tgcgcatact gctgaaatcc
    51901 accatgccca ggcataacga tgcgatatag ccgacacata gcccaatggc gatccctccc
    51961 atgcgtagca acggactgcg acagcagtta aagccgatca ccacaatcaa aaccaataaa
    52021 ccaacgccga gatgttcgta attgccgaac gtaccgctgc ttttggctgc aaatcctcca
    52081 ccaaaatcaa taatgccgac tttaatcagg cttaagccga tcatcagcac cacaatgccg
    52141 ctgacggtag gcgtaataac ccgacgtaaa tagggcagga taaaagaaga gccgacaacc
    52201 agaaatgcgc caacgaagga gacgccgagg agcgacgaca tgattaactc ttcgtgaaaa
    52261 ccgtcgcttt tcatgctgct gcccagcgca atcatgaccg taacaaatga aaaattgact
    52321 gactgaattg aaagtaaacc agaaccgacg atgccgtagc ggtttacttg taaccaggta
    52381 ccaataccag aggcgatcat cgccatagaa acaagatagg cagttgtttc agcggaaagc
    52441 tgtaaggccg cacccacgat taacgcgggg gttaccattg gaacgaaaat tgccaacaga
    52501 tgggtaatgg cacctacgag agcctgatga aagggagggc gatcctccag ttcaaatata
    52561 aggtcagaac ctgcatggtt tatatcagac atcccttcac tccttttaaa gtaaatttga
    52621 tcatctgaat tagtaaaata aattcccacg aatttggggc gcaggacgcc ttgagaacgg
    52681 cttatctcat tttcgcagtc actatttagt catcgttcaa acagagaaac aggccgccga
    52741 agcggccatt tattacttat attttttcca gcgcggtgag tattttctcc ggtgtgaaat
    52801 gccattcgcg taaccagatg ccgcaggcat cgtgaattgc ggtagcaatc gccggagctg
    52861 cgccatttac accgatttcc gagatcgatt tcgccccgaa tgggccgact ttatcgtcgc
    52921 tcggcaccag cacagcgcgg aaatcacgcg gaatgtcacc aattttcggt gcgccgtaac
    52981 tgcgtaaatc acgcgttaac gggtgacctt cggcgtcata aatgatctct tcgctcatac
    53041 tgtggccgat agctcgcaga gtggcaccgt agatttgtcc caacgctaac tctggattga
    53101 ccggtgtacc gcagtccagc aaggcgtaga atttatccag gcggatttca cccgtacgcg
    53161 tgttgacggc aacttcagcg aagtttgcgc catacgggaa ggcgaaatca ggcgtgatat
    53221 aactgccagt tcccaccagt gaaccaaagc cggtgccggt ttcgccttta tgggctatct
    53281 ctccgaaact aacttcgcct ttcttgccgc gaacgacgcc cggcgttgct agttgaacat
    53341 ctgccactgg ctcacccaac atttgcgcgc cgtggaacag gattttctcc cgcagatttt
    53401 ccgctgccaa acgcgcagcg ttacccgaga agcaagtacc ggacgaggca tatgcgcctt
    53461 tatcaaacaa cgcatgatcg gtatcaccgg agataacatg cacgtcctgc ggggggcagt
    53521 gcagcacttc tgctgccagt ttcgtcacca cagtatccag gccagtacca atatccgcac
    53581 cgccagaatg aacgataaag gtgccatccg attccagttt gatcatgcag ttagcctgat
    53641 cgatatccgg gatacccgat ttctgcatga taatggcgac gccgcgaccg atgtgccagt
    53701 caccattttg cggtttcggt gaagaccatt ggatcatctc gcgaccctga cgcaggatct
    53761 cttccagtgc gcagctggcg gcggaaggaa cggaggtcgg cgctttacct tcaccgatcg
    53821 caccgagaat tttcagctct tgcccttcgt gtacccggtt acgttcgata atttccagtt
    53881 ggtcgatctg taactgttca gccagttccg ctaatgccat ggtgatagcg aagttacctt
    53941 tcggtgcgcc ataaccctga taagcaccat ttgggcaaat gttgctgtag taggtggtga
    54001 cctggaaatc gacgttatcg catggatata acggcagcga cagcgccggt ccgttacacg
    54061 gtacggtgag tgagtggtta ccgtaagggc cagtgttggc gcggaaatcc atcttcactg
    54121 ccgtcaggcg accatctttt ttcgcgccca gtttgacggt gactttcgcg acgtggcgag
    54181 aggtgttagc aataaactct tcttcacggg tgtagcggaa cagtaccgga cgcccggtca
    54241 cgcaggttgc ccaggcgcac acttcttcca gcaggatgtc ctgtttggaa ccaaaaccgc
    54301 cgccaactcg ctctttaatg acatgaactt tatgctgttt catgcccacg aggcgcgcga
    54361 cctggcggcg taagtgccat ggtacctggg tggaggcgtg gatcaccaga cgatcgccgt
    54421 ccatacgggt aaagcagata tgtgtttcag tcgggcactg ctgtgcctgc gttgaattat
    54481 aggttcgctc aatgatcaca tcggcatcgg caaagccttt gtccatatcg ccaatatgac
    54541 catgaatact ggcagcgata tttttgcgtg ggcgagaacc gatcgggaag ttgatgatca
    54601 tatgctcgcc gcgctgggct gcatggctgt tatcatcttc cagagtctct ggcgcaccag
    54661 caacatacac caccggttca tcgtgcacga caggcgcatc ttccgccatt gcctcgtcaa
    54721 tcgacattac cggcttaagc acttcatatt caacgtcgat gagcttcagt gcttcgagcg
    54781 caatgtcttc actttcggct actaccgcag caacgcgatc gccgacgtga cgcattttct
    54841 tgccgaacat tcggcggtca agcggtgacg gttccggtgc gctctgacca cccggggtat
    54901 agtaaatatc cgggcaattc aggtgagtaa taacgtgaac gacgcccggt aaggcttcag
    54961 ctttgctgac atccagatga gtaatcagtg cgtgagcgtg tgggctacgt aacattttaa
    55021 tgacgcaggc gtcagccgtt acgcgatctt caacatagca gggtttcgcc tgcaccattt
    55081 tcgcggcatc agttttagga taatgcttgc caatgacttc aaggtcgtca cggaaagtcg
    55141 gagcgatatc aatggtggcc tgcggattat ttttacgtgc aaccgccagt tcaatgacct
    55201 gatagtattg ctgccagcca gcatcgcggc taaacagacc tgagagtgcg tcgtcgatct
    55261 cttcgcgggt aggtgcgtcg atgcgatcga gaagatcggt gataatcaga gccgcagctg
    55321 gatcgttata accagactgc accacgccaa catcaaccat ggcctgttga accagactta
    55381 actcattcca tttgcccaga gattctgcgg tacgaatatc tgccttctct aactgtgcgg
    55441 cgataagcaa ggacgcgtta acgatattac cgttaaagat aattgcgtca gaaccggcaa
    55501 acccaaaacc atcatcactg ttgcgtacag agtgcattcc catgttaaac aacagctttt
    55561 gcacgttttc gcctggatta acggttagct cctgaggcgc gccatttaaa gtaaagtgga
    55621 tgatcatacg gcttcctccc cagcctgttg gcagtcggca tacaggtcgg ctaccagaac
    55681 tcccgtgata tagcgtttat aggccacgct gccccgcaaa tcttcctgcg ggaaaatggc
    55741 gttggcgaca gcctgttcaa gtgcattgcc ttccagattt tgagtttcaa catcatgcag
    55801 acgcagtgct ttactggcga cgccatccag cgcaattcgc ataccgtcgt ggtctgttat
    55861 tgcaacggct gccgtcacga cggttaaacc tgcctgagaa cggctaattt tgcgagtcgc
    55921 acaggtgcga tacggatctt taatgataat ttcggttaac aggcgatcgc atgggcaggc
    55981 caggtagtcc tcgattgaca gcgtatcgcc gttgccaaaa accagttcag catccagtgc
    56041 cagcaggacg ggaagcagca ccgactcttc ctgccgggcg gcgatttcac caccaatggt
    56101 cgactgatta cgaacatggc gtgagtaaac aaaaccgagg gcttcacgca gtgctgcagg
    56161 aataaatcgc gcatcacgca gtgactgcaa gcgagacatt gcgccaattc gtagtgcacc
    56221 gttatcccag tcaatccagt ccagctccag atcctgcaag gaaatggcaa tctttttatc
    56281 ggtacgggtt ggtgtagcgt tgagtttgct gcccccggcg aaccagacgg cttcatcctg
    56341 gtagcggcgc ttcagttcca gcgcctgttc gactgagtcg ggtctgaaaa attgttcaat
    56401 catcgtcttt ccttaatttc ccctcttctc tgaaagaaga ggggagctgt tatttatgcc
    56461 agcgcatcca tccgacgcca catactggca gcggcttttc tggcttgcgc ataaatggaa
    56521 tcgcaatcga agttaaactg acggtcttca tagaccatca caccattgac catcacgctg
    56581 tgaacgctgc ctgagcccat accgaaagcg atatgcccgg cgatattgtc tgccagcagc
    56641 ggcgtcggcg agttgtaatc gcaaatggtt aaatcggctt tgtaaccggc ttccagaagc
    56701 ccaaatttcg cgccaaagtt gcggctcatc agttcgttgc cgttagccag tgctttggca
    56761 aaactgtcag gccacagcgg gccacccgca tcgcgatgtt taaagaaggc aaatttcatc
    56821 tcttcaaaca tgtccgaacc aatgccgtcc gttcccaacg ccagattgcg gatgtcgcta
    56881 aggtgatggt tgtagccgac atggttgttc atgtttgaac gggcgttatg caccaggaac
    56941 gcatcgcgct gattgagtag ggcgatgtca tctttcgaca agtacagccc atgagcgacc
    57001 agcgttttgc tgtcgatgag atcgaattgc gcaagtcgtg ccagcaggtc tttgccgtac
    57061 caatggtgac tgtaggaaac gtcgtaaagg tcttccgcaa cgtgaatatg caaaccacga
    57121 cctgtgcttt tcacggcttc acgcagcatc tccagaccgg catccggcac ggtaaacggc
    57181 gcgtgagcgc cgatatgtgc ttccaccaga tacggctcgg ttgctgcttt cttcgcctca
    57241 tcaatctgac gggcgaagcg gatgttttct tctacacctt cctgcaactc tttgatgccg
    57301 ctgttacggt cagtagtttc aaaacaggtc atcgcgcgca ggccaacttt taaaaatgcg
    57361 tcgcgcaatg tggagagcga cccgccgata taggctggag aggcatggtg atcgataacc
    57421 gatgtacatc cgctcttaat cgcttccagg gaacaaatca gtccgctgta atagagcgac
    57481 tcttcatcaa gggcgcgatc gagccgccac cagagatttt tcagcgttga gatgaaatcc
    57541 gggcaggggg cgatatttgc cataattccg cgggaaagcc ccgagtaaaa atggttatgc
    57601 gagcagacaa ttcccggcat cacaatccga ccatgcatct ctttgtagct ggcatcgggg
    57661 tagcgttgcg tcagggcatc gccgatagcg acaatcacat cattttcgat ggcgatatca
    57721 acgccttcct gcactttcgc cgggtgtaac tgcactgcgg tgacattctt cagaatcaac
    57781 atgattacac ctccacgcgg cccagcagat aatgatgatg ctggtggaca tggctaatga
    57841 tgcggcacat atcgttcagc tccggtggta cgttgttgaa ctgaccttcg ctgtcgatgt
    57901 ttaacaccca gctttggtta ttcagacgta cgcgtacccg gcaatcttcc acaaggaagc
    57961 ctgggttgct gctgttatca aagtcttgcg acaggctgaa gacggtgatt ttgtctttgt
    58021 acggtttacc gttccacggg cagaattggg cgcagttgcc gcattcgttg cagtaggcat
    58081 cgagatgcag cgtctggaaa cggttctgga agccaggaac cgcaatggat acgttggcgc
    58141 ggttcgggca gacatccaca cacttgctgc aaacgtagtt acattcaagg cagcgtgcgg
    58201 cttcctgtgc gacaaacgcg tcacgatcgt cactgttcac cagagtgaca gagatatcgc
    58261 ctttacgttg atagatttcc gccggattga cgttgttcca gtatttatcg ttctggtggg
    58321 aacggatatt ttcccgacta aggatggtat cggtcgcccg acgcgcggtt ccgacagcag
    58381 cgacaatgga ggatggtccg cgctgtacgt cgccgatcat aaagacgtca gtcagacgcg
    58441 tttcgccatt atggtcgacg tctggccagc cgtttttgtc cagcggcacg cccatcgcat
    58501 tcagggcttc agtatcctgc tgttcaccaa tggcggtgat cagactgtcc acatgcagtg
    58561 ttactgtttc gttggtttca accggacggc gacgaccttt ctcatcaggt tcgccaagcg
    58621 acataacgcg caaggttaag gtgccatcag catcgaaacg ttccggatta ttcaggaaac
    58681 ggaactccac gccgtcgtgc aacgcttctt catactcctc gcgccatgcg ggcatctctt
    58741 gcagtgaacg acggtaaacg accgttgctt tttctacgcc tggaacgcgt aacgccgcac
    58801 gagcgcagtc cattgcggtg ttacccgccc cgacaacgac cacatgtttg cccagcttga
    58861 gcgctgtgcc cttgttgtat tcacgcagga aggggagtga tttccagaca ttttggttgt
    58921 cgcccgccag tttcacaccg ctatttttat cagtgccggt ggcaatcaga acatagtgga
    58981 agccctgatt ttttaactgt tcaacggtca gatccggtga gcagccatac tcaaatttta
    59041 cgccgtgagc gccaacaaaa tcgatatcgt gctgaattaa ctctgcagga atacggaact
    59101 gaggaatgat atttttcacc acgccgcccg cattggcttc gcgttcaaac agcgtaaccg
    59161 gatggcccgc tctggcaagg aagtaacctg ctgccagacc cgccggacct gcaccaatca
    59221 cggcaaccgg atggcgtgaa ccagaaccgg ctggtttgtg ccagcgttgc ttatattcat
    59281 cccaaccttt ttccagcgcg acttttttca gttcgcggat attcagcgca ctgtcgtaat
    59341 ccaggcgggt acagttgtat tggcactggt gatcgcaaat atgaccggta atggcgggca
    59401 gggcgttgcg ttgatagatg agttccagcg cgtcggcata gcggtgttcg ccaagcagac
    59461 ggatgtattc cggaatatct tgcttgatag cgcaggcagt aacacagggg gcaacgtagc
    59521 aatcggtcag cggcaggtct tcagccactt caatacgctc ttctggcttc cagtgtttct
    59581 gggtgtattt catggttaac gcgtctgctg ccagtctgtt cagtcgttcg acgtcaacgt
    59641 ggttaagctc ccaggcatct gagctttcca gctcgcgcat gcaggcactt aagcgcagat
    59701 agccgccagg tttcagcagg tcggttgcca tagtaatagg gcgaatacca gtatcaaaaa
    59761 tatcgcggat agtcagctga ctggcaccac cggaataaga aatgggcagt ttgccgtcaa
    59821 aggcgcgaga gagaactgct gcaacgttga tggagagcgg gaacagcgca cggcctgaca
    59881 tatacatctc ttcaccgggc aatgcgcctt tattgttgat ggtgccgaga gtgttagtca
    59941 gttttacgcc aaagccgagt gatttttctt ttgccagtgc catcaggcgt tccagcattt
    60001 ccagtgcttg cgtcagcttg aggtcgtgat caaatgactc ttcttttaag ccgatgtaac
    60061 cgaaaccgca ggcatcgagg atctcacgaa cacgcgcgta ccccagtaag gtcgggttga
    60121 gtttcacaaa ggtgttgagc cctttttctt ccagcatgta gcggcaaatg gcttcgattt
    60181 catgcggagg acagccgtgc atggtggaga gggtaacccc ttgtaccatg ctggtgggga
    60241 tgcgagcggg taaggcttgc aagctttcgc gtttttcctg caatccgtgg cgggctaaaa
    60301 atgcctcatc ctggagtagc ttattaagtg catcgcggta ttgagcgaat ttcggatggt
    60361 cagatgcgtc catcatattg tcgatgaact gctgcatcgg cggttgctta ataccttcga
    60421 ggttgtaacc gacgctcata ttaaagatga acgatttacc ggaatcagaa ggctggaaca
    60481 tcgcttcgag aaggtgcagg gcaaaccagg ctttgaggta ttcatcccag gctttaagca
    60541 gggtaaattc ggtagaccat tcggtgttaa agcactcgtc ttcggcatcg atacagggct
    60601 tttccagctc cagacggtca agaatttgga cggtttttag ttcgatgaag cgtccgccag
    60661 tcagccagga agtgacaata ttttgcgcga gttgcgtgtg cgggccagcg gcagggccga
    60721 cgggagtggc acaggtttca ccgaagacgc taacagtttt accttttacg ggtgagtaaa
    60781 actgttgctc gggaatacca aagattgagc gttgttgttg gtattcatca aatatgcgcg
    60841 tcaaaagttc ctcaaacgga atgggacgca taatatcccc cataaccctc tccttagctt
    60901 atacagatat cattgagtga ccggtgttga gaaatccggt ggtttagggg gatagagcaa
    60961 caatcgcacc actcttgcca ggtttggcgg gaaatataaa aaaatgtgag gcggctcgaa
    61021 acttaatggc tttttgtgaa tgaactcaca ggtgccaaat gagaattaaa atcactcctg
    61081 aaaattatta ttgccagccc atttatcact ttttctcttt tctcaaaatc agtgattcct
    61141 tatcattatg ataaagagtg ctggagggat taaatatttg aacgcaatcg tttaactacg
    61201 ttttctaaat ttcagtatac cttttcttcg ctgtaataaa gtcgtccgga gtatcaatat
    61261 ctaaaattat ttctgcattt tctatttcga cggaataatg ttctcccata aggagtgcct
    61321 gacgcatatt ggtgacattg gggcgctgga ttgcctgcat caggcatggt tttgacacta
    61381 aaatcggatg gccggggata ccattatgga gtggcagtat tgcgccatca tttcgtaacg
    61441 accagatttt tctaaaaata tcgatggtga gggttggcat atctccgtgg gtgagaaaac
    61501 aatgttctgt ttgcaccgcg ggtactgcgg cctttactga tgtcagtaaa ccctgcgcat
    61561 aatctgggtt gtggataata gtgatattgc tctggttcgc ataacgttcg tgcagttcat
    61621 taccacgata gccggtgact aaaataattc ggctacaaaa ctgcaacgca tttttgatac
    61681 ttgtatcaag aattgttccc tgttgccagg gtaacatcat tttccattgc cccattcttg
    61741 atgataatcc tgcggcggta attatacagt cgatggctga catagtgatt ccattattta
    61801 caggagctaa taacgcctga taattgcgtt acttcctgat ggattacgtt gagggtaatt
    61861 tatcccagag aggtccataa agacgcgtga aaagtataat tgactcatcg gcattattca
    61921 ttgatttagg cgcgcagaaa cgtcccactg tgatttctat tgttggtgct ggggggaaaa
    61981 ccagcttgct tttttggctg gcagaactgt tccaggcgag cggcaggcgt gtattaatca
    62041 ctacgacgac acatatgttt atgccaacat ctcactggcc cgtggttttc tgtcgtgatc
    62101 ccgccatgct tcctcatgcg tcttttatat cccccatttc attttgtttt cacagctgga
    62161 aagcgaatca gggaaaagtg cagggattta cgccagaagc gattgatgca ctggtacaac
    62221 gaccagagtg tgacgtaatt ctcattgagg cagatggctc gcgtggaatg ccgttaaaag
    62281 cgcctgatga gcacgaacct tgcataccta aaagcagttg ctgcgtgatt gctgtgatgg
    62341 gaggacatat tttgggtgca aaagtgagca cagaaaatgt ccatcgctgg tcgcagtttg
    62401 ctgatattac tgggttaaca cctgatgcac ccttgcaact gagcgatctc gttgcgctgg
    62461 ttcgccaccc tcagggcgcg tttaaaaacg taccgcaagg ttgtcggcga gtctggttca
    62521 ttaaccgttt ttctcaatgt gagaatgcga ttgcgcaaag cgagctcctt caaccgctgc
    62581 aacaacacaa cgtagaggca atctggctgg gcgatataca agaacatcct gcaatcgcgc
    62641 gcagatttgt gaattagcgg cccgggtatt ccgggttact gaaaggatat ccattgaggt
    62701 tggtatgaat attttcacag aggctgcaaa actcgaagag caaaattgtc cgtttgcgat
    62761 ggcgcaaatt gttgatagcc gaggctcaac cccccgccat tctgcacaaa tgttagtgcg
    62821 cgccgatggt tctatcgtcg gtacaattgg tggcggaatg gttgagcgga aggtgattga
    62881 agagtcgctt caggcattgc aggaacgtaa gccgcgatta ttccatggac gtatggctcg
    62941 taacggtgcg gatgctgtcg ggtcagattg tggaggtgca atgtcagtat ttatcagcgt
    63001 ccatggtatg cgtccacgtc tggtgttgat tggtgcgggg catgtcaacc gggcgatagc
    63061 ccagagtgcg gcgctattag gatttgatat cgccgttgcc gatatttatc gcgaaagcct
    63121 caatcctgaa ctattcccac catcaaccac gcttctccat gctgagtcgt ttggtgcggc
    63181 agtggaagca ctggatattc gccctgataa ttttgtcctg attgccacga ataatcagga
    63241 tcgtgaagcc ctcgacaaac tcattgaaca acccattgca tggctggggt tgctggcaag
    63301 tcgccgcaag gttcagcttt tcctgcgcca attgcgtgag aaaggcgtgg ctgaagaaca
    63361 tattgcccgt ttacatgcgc ccgttggtta caacataggt gcggaaacgc cgcaggagat
    63421 cgccatcagc gtgctggcag aaatattaca ggtgaaaaat aacgcgccgg gtgggctgat
    63481 gatgaagcct tctcatcctt ccggacacca gctggtggtg attcgcggtg cgggggatat
    63541 cgccagtggt gtagcgctac gtctgtatca tgcgggtttt aaagtgatca tgttggaagt
    63601 ggaaaaaccg acagtgattc gttgtaccgt ggcgtttgcc caggccgtgt tcgatggcga
    63661 aatgacggtc gaaggcgtca ccgcccgcct ggcaaccagc tctgcggaag cgatgaaact
    63721 taccgaacgc ggatttatcc ctgtgatggt agatcccacc tgttcattgc ttgatgaact
    63781 gaaaccgctt tgcgtggtgg acgctattct ggcgaaacag aatttgggaa cgcgggcaga
    63841 tatggcacca gtaacaatcg cgcttgggcc gggctttact gcagggaagg attgtcatgc
    63901 ggtaattgaa acaaatcgcg ggcactggct cggtcaggtg atttactcag gttgtgcgca
    63961 ggagaatacc ggtgttcccg gcaatattat ggggcatacc acccgacgag tgatccgcgc
    64021 tcctgctgca ggcattatgc gatccaacgt gaaattaggt gatctggtga aagagggcga
    64081 tgtcattgcc tggattggtg agcatgaaat taaagcaccg ttgacgggga tggtgcgtgg
    64141 cttgttgaac gacggactgg ccgtggtcgg tggttttaaa attggtgata tcgatcctcg
    64201 tggtgaaacg gctgatttca ccagcgtttc tgataaagcc cgggcgattg gcggcggcgt
    64261 acttgaggcg ttaatgatgt tgatgcatca gggcgttaaa gcgacaaaag aagtgctgga
    64321 agtggcttaa atgaaaatgg ccggatggta ttacccatcc ggcctttaaa ttactgacaa
    64381 atcaccgttc cggttttgcc ttcaatccct tctttcgctt tgctcaatac ggtaatcagt
    64441 gcttcacgac ctgcgcgaga gcgagcaaac gaggcggcag cttccacttt aggcagcata
    64501 gaacctttcg caaaatgacc ttcttcgata aagcgttcag catcacttaa tgacaggcga
    64561 tcgagccact gttcattctc ttttccgaag ttaatggcca ctttttctac cgcagtcaga
    64621 atgatcagca tatcggcatc gatcatttct gctaaacggg cactggccca gtctttatcg
    64681 ataaccgcgc tggcaccacg cagatggtta ccttcacgaa taacagggat accaccaccg
    64741 ccaacggtga tcaccacctg tccggcatct accagagctt taaccgtttc tttttcaatg
    64801 atatcaaccg gttttggcga agcaacgaca cgacgatagc cgcgacccgc atcttctttc
    64861 agagtgtaac cctgttttgt cagtagctct gcttcttgct cggtaaagaa cgaaccaatc
    64921 ggcttggtcg ggttgaggaa tgccggatcg ttagcatcga cttcgacctg ggtaaccagc
    64981 gttgctacag gtttattaat gccacgagaa agcagttctt ctcttaaggc gttttgtaga
    65041 tcgtaaccaa tataaccctg gcttaaggca acacagacag acatcggcag cattggcgag
    65101 tgcgcttcag tttttgcggc agcttcaaag gcctggttaa tcatgcccac ctgtggcccg
    65161 ttgccatgag tgacgatgac ctcatgtccc tgggcaatta aatcaacaat tgcctgagaa
    65221 gtaattttta ccgctttcat ttgtccggcc aggtcatcgc ccagcgcatt cccgcccagg
    65281 gcgagaacaa ttttcttact catttttcct cactcatcat tcttgattgt gtatagatcc
    65341 ctggcagtgg ttagagcacg ggagggacaa acggtttgcg tcgcaggaaa cgacctcgcc
    65401 cggctttgcc tgtaaaggtg ccgtcgcaga aaatcgtttc accacgggat aaggttctga
    65461 caatcgcgcc ctgacaggta aaaccctccc atggcgagta gtcagcgttg tcgtggagat
    65521 ggcgatgctg aatttgttgg ctctgacgtg ggtctataat caccacgtcg ccatcggaac
    65581 cgggcgctaa taatcctttt tgtggccaca ggccaaacag cctggcgggc attgcactgg
    65641 ttaactcaac aaaacgctcc ggtgagatac gccccgtcat cacgccactg gagaacagca
    65701 gttgcatgcg gttctccaca ccgggtaagc catttgggca gcggctgaaa tcgcctttag
    65761 aaatttgcag gcgttgagcc atcgagaagg tgcagtgatc ggttgccacc acgtcaatcg
    65821 caccatcgct gatgccacac cacagtttgt cctgctcgcg tacgttacgc agcggtgggc
    65881 taagaatgaa cttcatgcca tcttctgtat cgtaactgcg ttcgtccaac aggagatatt
    65941 gtgggcaggt ttcaacccag actggctggt gattcgcacg ggcaagacgc agataatcca
    66001 gacctaagcc gttagacagg tgcacgatat agagcggggc gttaccggca atttgtgcca
    66061 ggttaatcat gcgggcgatg gcttccgctt cgcattccag aggtcgactc aaggcatgat
    66121 aacgcggcgc ggttaaccct gcggcgataa actccgcccg cttgctggcg atagctgcgt
    66181 cattttccgg gtgcacggtg gtcagcgcgc cggactcatg caagcggcgt aatgcctgca
    66241 acacttcatc atcgttgagt ttatattgat aggttaaata gagtttaaaa ctgctcagtc
    66301 cttcctcaac catcatcgga atttcgtcga ggatagcgtg attaatatgt tgaatgacac
    66361 cgtgaaagct gtagtcgatg actgctttgt gggcggcata accgcgataa acttccagtt
    66421 gatggcgtaa ccggcagcca tttgggccaa atcccatatg gtcaataatg gttgttgtac
    66481 cgccacacgc agctgcgcgg gtaccggtaa aaaaatcatc acaactgcgc gcgatgccga
    66541 catcaatatt gaaatgcgta tggacatcca cgccgccagg gaaaacgtaa cagccagagg
    66601 catcaatttc ttcgcaaggg agttgcggcg aaatatcggt acccaactgg cgaacaatcc
    66661 cgctttcaat cagcaaatcc tgcttcgcct gtccatctgc attaacagca atgccgtttt
    66721 ttatcaatac gcgcatagca aactccaaaa accctccggt gaaccggagg ggaataagtg
    66781 aagagtaatt attcggttgc cagccagctt aatgggattg cagcgtacat agcggcacag
    66841 gttaccaggt gagatttcca ggttttttcg tttggcgcgt gcgcttccgg ttctttaccg
    66901 gggccaaaac cgatgaccgg aatgccgtgg cgacccatga tggatacgcc attagtcgag
    66961 aaggtccact tatcaacgac aggcgcttta ccaaacagcc cttcataagc attcaccagt
    67021 gctttaacgg tgaagtgatc ttcttccact ttccaggtcg ggaagtagca ttcggttggg
    67081 taaaccaggc cagtccagga cgggcggtcg tagttgtaca tagaaacgac agcattcgct
    67141 ttttgtactg caggcagggc acggatctcg tccagcgcgc cttcccaggt ttcgccccag
    67201 gtcagacgac ggtcgattga aactgcgcag ctgtctgcga cagcgcaacg gcttggggag
    67261 gtgaagaaaa tttcagaaac ggtgagggtg cctttgccga ggaattcgtc ataacccaga
    67321 cgttgggaga gttcttgtaa ttcgccaaga atcggaccca ttttgaaaat ggcgttatca
    67381 ccgcgttctg gtgccgaacc gtggcagcta acaccctgaa catcaatacg aatttccata
    67441 cgaccgcgtt gaccacggta tacctggcag tcggttggtt cggtactgac cacaaattcc
    67501 gggcgaatgc cggattgttc aataatgtac tgccagcaca ggccgtcgca gtcttcttcc
    67561 tgcacagtac cggtaaccag cagggtatat tcatcttcca gaccgagatc tttaatgatt
    67621 ttaccggcat ataccataga cgccatgccg ccttcctggt cggaagtacc acgaccaccg
    67681 atcagctcgt cggtttccat gccttcatac ggatcgaagt cccagttttt gatgttgcca
    67741 atgccgactg tatcgatatg agcgtccatt gccaccagac gcgggccgtg gccgatataa
    67801 ccgagaacgt tgcccattgg gtcgatttca actttatcga agccgacttt ttccatctct
    67861 tctttaatac gatgtactac gcgtttctcg tcgcagcttt cactgggaat agcaaccata
    67921 tcgcgcagga agcgagtcat gtctgcctgg taatcttttg ctttttcaag aatcagtttg
    67981 aatggaatat tcttagccat tatttttctc cagatttttt actccccgtt ttgcagggag
    68041 cagttccaat agggtgatta aggtgctaca gcgtgtttgc cttcccagac aacttcgcgg
    68101 tagtgcttca cgtcggtgtc gccttcagtg ctgataacca gcaccacggc atctttgttc
    68161 agcgccagtt tttccatcag gctttgacgt tgcgggtgat aatgaaccgc tgcgagaacg
    68221 cccaaaccga cagcgccgga ttcaccggag atgatgcgcg ggtcgttgcc gtacggatta
    68281 cccagcacgc gcatacctaa tgcggcaacg ctgtcctggc aggagatgaa ttgggtggcg
    68341 cagttacgta ggatttccca gcccagcggg ttaggttcgc cgcaggccag gcccgccatg
    68401 atggtggcca tatcaccgcc aacgttgacg atgtcgcctt tgacgccgga gcgataaata
    68461 cagtcagctt tgtcaggttc aacaataatg ctgtgcagat tttgcgggct atagacgtcg
    68521 accagataac ccagcacacc accggccatt gcaccgacac ctgcttgcag cagaacgtgt
    68581 gtcggggtta cgcccatttc acgcatttgc tcgacggctt catccgccag tgttgcgtag
    68641 ccttgcatga tccaggttgg gattttggtg taaccttccc atgccgtgtc ctgtaccact
    68701 tcccagccgt gctgctgcgc gtgttgcatg gtcaggcgaa cggtatcgtc atagttcata
    68761 tccgtgacga tgcactcggc accgaggttt agaatggcgt caacgcgttc ctgagcagaa
    68821 cctttcggca tgtaaatcac cgcattctgt ccgagttgct gagcggccca tgccacaccg
    68881 cgcccgtggt tgccgtcggt ggtggtcgcg aaagtcattt tttcgccaat ggcatttttc
    68941 aggtgctcaa atgatagcgt ttcgatatca agatgatatt tttcacacaa taattgagcg
    69001 atggcgtatg cgccgccgag cattttgaag gcgttcagac cgaagcgttt tgattcgtcc
    69061 ttaacgagaa ttttctttac accaaacagg tttgcgagat cgtccagcgc acaaagcggt
    69121 gttgggcgat aaccagcaat tttctggtgg aactggcgtg ccagttttgc ctggctttgc
    69181 gaaaaaagcg gtgatgtttc gccgttgaaa aatttgttat cggcgatatc aatctttaat
    69241 gagaaaacgg acatatccta tccttttggc ctctggaaat agagggccga ccagctgatc
    69301 ggcccgaggg gttatttcac gcgttcttgc gcttctttca gcagttgctc gagcagtgca
    69361 cctggtttgg cgtatttacg ggacaggatc atcgcggcga tgatataagg cttccagctg
    69421 gcttctttgt aggtagcgat acggtatttt tcgaatacgc cttcagtcac ttcaccttct
    69481 ttgcaggata cgccgctgat atcagctggc aggcagtgca tgtacagggc ttcgccatca
    69541 cgggtcagct ccatcatctc ttcggtacaa tgccagtctt tgtgctgtgc gttctgtgcc
    69601 agacactgtt tttccagcgc tttcagtcct tcgtgatcgt tcgcacgcag cagttcagta
    69661 cgctcttcca tcactttgta aggtgcccag gacttcggat aaacgatgtc tgcgtctttg
    69721 aaggcttctt ccatgctggt gacctgacgg aagctaccac cggaggcttt agcattgttt
    69781 ttagcaactt caacgacatc cgggatcagg tcgtagcctt ccggatgggc cagggtgaca
    69841 tccataccga agcgggtcat cagaccgatg atgccttgtg gtacagagag cggtttgcca
    69901 tagcttgggg agtaggccca ggtcatggcg atttttttac ctttcaggtt ttccagtgaa
    69961 ccaaagtgtt cacgtaacca tgccagatca gccattgatt gagtcgggtg gtcaatatcg
    70021 cattgcaggt tcactaaagc cggacgctgc ggcagcacac cctgcttgta accgtcatca
    70081 agtgcagcgc caacttcacg catataggcg ttgcctgcgc ccagatacat atcgtcgcga
    70141 ataccgatag cgtcagcgca gaaggagatc atattggcgg tttcacgcac ggtttcgccg
    70201 tgagcgattt gtgatttacc ttcgtcaaga tcttgttgtg caaggccgag caggttaagc
    70261 gcggaagcat aagagaagcg ggtacgggtg gaattgtcgc ggaatacgga aatacctaat
    70321 ccactattaa agactttggt tgagatgttt tcagcacgca gtgcttttaa cgcggcggca
    70381 acgtccagta cttgtttcag ttcgtctggc gtctgttccc acgttaacaa aaaatctttt
    70441 tcatgcaggt gagaggtcag cgaattgata tccttaatca gctcattaac agttttcatc
    70501 ataaagtccc gatagtggaa tgacaggcaa agtggtttat ttcactgtga cgtgccaaag
    70561 tgattggtgt tttcacagct atacctcgga ctaacaataa gtgtgccaga attatccacg
    70621 cggctgcgaa aggctaattt gacggataat gtgtggggtt gatcacatat tgatgccagt
    70681 ttccggttgt ttgtaaaata cgaaaacgtt tggctttttt tattacgtta tttatttgct
    70741 gctaagtatc agaaataaga taaagaggca ggctatctct cttcagtttg ataaacttgc
    70801 cgctgtaatt ctcatattga tatagaaatg tgatatctct tccataattt tattagaaag
    70861 tgataaaaat cttatgtttt tatcaaagcc aatttcgcct gagttaatat catcatgatc
    70921 tgtgaaccat caacgtcttc aatgatcgac ttttaagtaa gtctggaagg taaagctatg
    70981 gagcttgcga ctacgcagtc agtattgatg caaattcaac caacaattca gcgttttgcc
    71041 agaatgcttg ccagcgtttt gcagcttgag gttgagatcg ttgatgaaaa cttgtgtcgc
    71101 gtcgccggaa cgggcgcgta tgggaagttt cttggccgcc agttgagcgg caactcacgt
    71161 ctgctccgcc acgtcctgga aacgaaaact gaaaaagttg tgacacagtc tcgcttcgat
    71221 cccctttgtg aaggttgcga tagtaaagaa aattgccgcg aaaaagcatt tctgggtacg
    71281 ccagtcattt tacaggatcg ttgtgttggg gtgataagtt tgattgccgt tacccacgag
    71341 caacaagaac atatcagtga taatttacgc gaattttccg attacgttcg ccatatatcc
    71401 accatttttg tttctaaact tctggaggat cagggaccag gagataacat cagtaaaata
    71461 ttcgcgacca tgatcgataa tatggatcag ggcgtgttag tggttgatgc tgataatcgg
    71521 gttcagtttg ttaatcagac agctttaaaa acactcggtg ttgtacaaaa taatattatt
    71581 ggaaaacctg ttcgtttcag accattaaca tttgagagta attttaccca tggtcatatg
    71641 caacatattg tttcgtggga tgataaaagt gaattaatca ttggtcagtt gcataacatc
    71701 cagggccgac aactattttt aatggcgttt caccaatcgc ataccagttt ttctgtagca
    71761 aatgcacctg atgaaccgca tattgaacaa ttggttggcg agtgccgtgt tatgcggcaa
    71821 ttaaaacgac tcattagccg tattgcaccc agcccatcca gcgttatggt ggttggtgaa
    71881 agcggcacgg gtaaagaagt cgtcgcccgt gcaatccata agttgagcgg aagacggaat
    71941 aaacccttta ttgctatcaa ctgtgccgcg attccggaac agcttctgga gagcgaactg
    72001 ttcggttatg ttaaaggcgc atttactggc gcttctgcca acggtaaaac agggttgatt
    72061 caggcggcga atacgggcac gctgtttctc gatgaaattg gtgatatgcc attaatgttg
    72121 caggctaaat tactgcgcgc tattgaggcg cgtgaaattc tgccgattgg tgccagtagc
    72181 ccaatacaag tcgacatccg catcatttct gcaactaatc agaatttggc ccagttcatt
    72241 gccgaaggta aattccgcga agatctcttc taccgactta atgttatccc gataactctt
    72301 ccaccgctgc gtgaacgtca ggaagatatt gaattactag tgcattactt tttacatctg
    72361 catacccgtc gtctgggatc ggtttatcct ggcattgctc ctgatgtcgt cgaaatattg
    72421 cgtaagcatc gttggcccgg aaacctgcgc gagttaagca atttgatgga atatctggtt
    72481 aacgttgttc cttcaggtga agttatcgac agcacgctat tgccgccaaa tctgctgaat
    72541 aatggcacaa cggagcaaag tgatgtaaca gaggccagtg aggcgcacct gtcactcgat
    72601 gatgcgggcg gcacggcgct ggaagagatg gaaaagcaaa tgatccgcga ggcgctttca
    72661 cgtcataaca gcaagaagga agttgctgat gaactgggca tcggcattgc tacgctctat
    72721 cgcaagatta agaaatatga gttgttaaac acataacctc tgagaatccg gcctgaattc
    72781 aggccggata tcttttactt tgttttctcg caatccagaa ctgtatttac aatcatctga
    72841 taccccgtgc agcgacaaag atttcccgcc agtccacgac gaatttccgt aatggttaat
    72901 ggcttctcgc gtggtttcgc cagcattgcc gtggtagcca taatcaggcc cggcgtacaa
    72961 aacccgcatt gcactgcacc agatttcgca taagccagtt ggacatgaga aagtttaccg
    73021 cctttcgctt caccttccag cgtgcggatc tcttttcctt cagcccaggc cgcaaggaat
    73081 aagcaactgt ctatcgcagt gccgtcgacc agcaccgtac aggcaccaca ttcacccacg
    73141 cagcaccctt gtttgacact tagcagtcct tgttcgcgga gtaattccga gagcggcgtg
    73201 cctggtgcgg cgtgaagctg aaaaggcatc ccgttaatgg tgcattcgat ggtaattgtt
    73261 tcgctgtgat tcattgcaat ttcccccccg ccgcggcgac ggcttcgcta atcacttttt
    73321 tggtcatcgt ctggatgaga tgcagacgaa actctttact ggcccgccat gacgaacgcg
    73381 gggcgacatc ttgcaggact gattcgctga tggcttccag cgtttgcagg tttaatggcg
    73441 cattttgtgc agtctgttcg gcatgttggc agcgaatcgg cgttggcgcg gcaacaccaa
    73501 atgccaggcg taattcgctg aaattgccgt tatccagtcg gcaatgtgcg gcgcagccaa
    73561 ttgttgaaat atccattgcg tcgcgcatgg catatttaaa atgcgcgctg cccgcgtgtt
    73621 ctttcggctg tggcggaaaa tgaaaggcga caaggatttc gtcatgctca agagacactt
    73681 tgcccggccc ggtgtgaaag ccattaatcg ggacgaaacg aacaccgcgc ggggagtgga
    73741 tctccagttt cgcgtcataa attagcgttg gcgtggcaga atctgcgctg gtggcaccgt
    73801 tgcaaatatt tccgccgtag gtggcgacat tacggatctg cggcccggcg attgatgcag
    73861 ccgcagcaca taacgccggg agatgacgtt gagttacagg atcttcaatg agctgggtaa
    73921 atgtcgttgc agagccgata cgtagcgcgc catcttccgc cagcgtaatt ccccgcagct
    73981 ccgccagatt atgaatatca acaatatggc gataacggtc attgtgatgg tgaagctgta
    74041 tcagtacgtc agtgccaccg gcgagcagtt tggcctgcgg gttgtcagtc agcaggttga
    74101 tggcatcggc aagggttgct gcgcgatggt aagaagcaaa atcaaacatg atgttatcct
    74161 caaatcaatc ctgccagatg gaattcttca tataatcgtt ttggcgttag cggcagtgta
    74221 ttgattgcaa caccggtagc catcttcacc gcgttacgaa tagcagcggc tacaggaatt
    74281 atgggtggct cacccagtga cttatgtccg tatgcggatt gcggctcatt gatttcgacg
    74341 aacgcgcttt ccagttgtgg cagatccggc atggtcggca ttttgtaatc cagcagattg
    74401 gggttacgga ccacgccgct tttcgcatcg atgatcatct cttcaaatag cgcccagcca
    74461 atgcccatcc ccattccgcc gtgtacctga ccttctgcca gcagcggatt aagaatatgg
    74521 cccgaatcat gaacgttgag gatgcggttg atggttactt tacacagtgc aatatcgacc
    74581 gtcagatcaa caaaggtaca gccaaacgcc ggtgggttag tggtggtttt gatggagctt
    74641 tcggcagaga gctgcccgcc gcgttcaggg tggtagaaag cgtccatcgc caaatctttt
    74701 aacgacatta acggctcttc cggtcgttca accagcacga tatggccttt tatcagggtc
    74761 agattcatcg ctgactgatg tagcatgact gcggcgtgag cgatgatttt ctcttttaat
    74821 aacagtgccg cactgcgcag cgcaggcgcg gcaacatagc tctgacgtga ggcaaatgcg
    74881 ccggggtcga atggtgtaac gtcggtgtct tgtgtcgaaa ttacgcggac atcgctgacc
    74941 ggaactccca cggtttctgc caccatttgc gagaacacgg tgtcggcacc ctggccgatt
    75001 tccgtcgcgc cgctttgcac gttgatggtt ccatcctgat tcatcaacag gcgcgcgcct
    75061 gctatttcta cgccgacagg ccaggtgtta gaggtgtagc taaaacaggc gacgccaacg
    75121 ccacgacgta aattgccttg ctggttctgg cactctgcac ggcgtttttc ccattcaaag
    75181 attttccggc ctttttcaag acactccggc aaccctgcgc tgtaaatacg ttttcccgtg
    75241 agcggattag catctccttc gcgggcggcg ttgcgtaaac gaatttcaac aggatcaata
    75301 cctaacgctg tcgcggcatc atcaagcata gactcaacgg caaatacgac ttgtggcgcg
    75361 ccataaccac gcatcgcacc agccgagggg aggttggtat agcaggtctt tgaactgtaa
    75421 gcgtaggcac aacgaggata aaggtaagcg actttattcc ccccagcaga agcaatggag
    75481 tgcccgtgag atgcataagc gccggtgtta gacagaacat ccagactata acctttcaat
    75541 gttccgtcgc ggttcacgcc catttgcccg tcaatagtaa aagcgtggcg ggtacgggtt
    75601 gcgaggaaac actcttcacg gctaagagaa actttcaccg gaatgccgcc aagtttgctt
    75661 gtcaggaatg ccgccattgg ctcttccagt acatcctgtt tattaccaaa accgccaccg
    75721 ataaacggtt tgatgactcg tacgcatgac cagggaatat ccagcgcctg accaaccacg
    75781 cggcgaacaa tgtgcgggat ctgggtgctg gaaacgatgg taattcgcga gtcatcctcc
    75841 atccatgcca gcgatgtcac gctttccata tgacaatgtt gaataacggg cgtctgatag
    75901 tgcccctgta cctggtagtc ggcggcatcg attgtttgtt ggacattacc cgtcgacatc
    75961 gtgctttgtt tcagtaaatt gccaccgtta tggattggtg cagcgtcttc tgccagcgcc
    76021 gcttctgacg aggtgataac gggtaattct tgccactcaa tgctgaccaa ttgcaccgct
    76081 ttttctgccg tgagttcatc gcgggctacg acgatggcaa cggcgtcacc atgatgacga
    76141 acatgacgcg ttagcagggc acgatcggca gtatcgcgct tgttttcgtc aagtgtccag
    76201 gcatgccctg ccgtggcgaa tgggatttct ggcacatctt cccaggtaaa aatcgccagg
    76261 acgcccggca aactcctggc ttgttcatca ttaatactta cggcataacc atgtgcgata
    76321 gggctacgta catatttcgc gtaacacatg cccgccataa cataatcgtc agtatatcgt
    76381 gcccgcccgg tgaccttagc aatggcatcg acgcgcatgc atgattcacc cgtagcggtt
    76441 gcttcccgcg cttccataga aatcccctcg gtttgaacat ttggttctta tggcaaagat
    76501 ttagtcttag caggcaagaa ttacgccaga aacgactata tgcgtgaggg gaggaaggaa
    76561 gtgtgatgac gttaaaaaaa cacagatatg atacgaaaca ggactctgaa tagggacagt
    76621 gatacgtatc aatatgagtg gcgataaaat caatgtgata aattaggatt attacgcatc
    76681 aaggtgggaa atagggtaaa agtcaggaaa ttcgatacca ctacagcaaa tattttgctg
    76741 atgatacttc cctttgccaa atgaaattaa cctctttctc tattacatcg tttttccaga
    76801 agcaagagat tgggtatcaa gaggctggct gctatgatat ggcgtcttgt tttttaagcg
    76861 aggaaagatt ttgagtgcgg gacgcctgaa taaaaaatct ctgggtatcg tgatgttgtt
    76921 atcggttgga ctgcttttgg cgggctgttc gggtagcaaa tcatccgata caggaacgta
    76981 ttccggctcc gtttacaccg tgaaacgggg ggatacgcta tatcgtattt cgcgcaccac
    77041 gggaaccagc gtaaaagaac tggcgcgact gaacggcatt tccccccctt acaccattga
    77101 agtaggtcag aaactaaaac tgggtggggc gaaaagtagc agtagtacac gtaaatcaac
    77161 cgccaaatcc acgaccaaaa ccgcatcggt tacaccgtca tcagcggtac cgaaatcttc
    77221 ctggccgcca gtagggcaac gttgttggtt atggccaacg acagggaaag ttatcatgcc
    77281 gtattcgaca gcagatggcg gcaataaagg gattgatatc tcagctccac gaggtacacc
    77341 tatttacgcc gcgggtgcag gaaaggtggt gtatgtgggc aaccagctgc gtggctacgg
    77401 taatctcatc atgattaaac acagtgaaga ttacattacg gcttacgctc ataatgacac
    77461 gatgctggta aataatgggc aaagcgtgaa ggctgggcaa aaaatcgcca ctatggggag
    77521 cactgatgcg gcatctgttc gcctgcattt ccagattcgt taccgtgcaa cggcaattga
    77581 tccgctacgt tacttgccgc ctcagggcag caagccaaaa tgctgatggc gaattaatca
    77641 gcagtcagca gcgtggtact tgctaaggag agcgtaaggt ttataatgcc ttacgcatct
    77701 cgaagcgggc gtagttcaat ggtagaacga gagcttccca agctctatac gagggttcga
    77761 ttcccttcgc ccgctccaat ttatcttcac atcatctacc tgccattttt gttgtatctg
    77821 attttctata ttatcttaat tcaagcgctt cagacttttc gcaatcgccg taatacttcc
    77881 ccattttttt gtaaataaaa agcgtttacg caatgcaatc gtcatgtat
//
LOCUS       DAHHTG010000005        73140 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.005, whole genome shotgun sequence.
ACCESSION   DAHHTG010000005 DAHHTG010000000
VERSION     DAHHTG010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 73140)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 73140)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..73140
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.005"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            complement(214..1689)
                     /gene="ampG"
                     /locus_tag="NBI74_000356"
     CDS             complement(214..1689)
                     /gene="ampG"
                     /locus_tag="NBI74_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muropeptide MFS transporter AmpG"
                     /protein_id="HCD1930734.1"
                     /translation="MSSQYLRIFQQPRSAILLILGFASGLPLALTSGTLQAWMTVENI
                     DLKTIGFFSLVGQAYVFKFLWSPLMDRYTPPFFGRRRGWLLATQILLLVAIAAMGFLE
                     PGTQLRWMAALAVVIAFCSASQDIVFDAWKTDVLPAEERGAGAAISVLGYRLGMLVSG
                     GLALWLADKWLGWQGMYWLMAALLIPCIIATLLAPEPTDTIPVPKTLEQAVVAPLRDF
                     FGRNNAWLILLLIVLYKLGDAFAMSLTTTFLIRGVGFDAGEVGVVNKTLGLLATIVGA
                     LYGGILMQRLSLFRALLIFGILQGASNAGYWLLSITDKHLYSMGAAVFFENLCGGMGT
                     SAFVALLMTLCNKSFSATQFALLSALSAVGRVYVGPVAGWFVEAHGWSTFYLFSVAAA
                     APGLILLLVCRQTLEYTRVNDNFISRTEYPAGYAFAMWTLAAGISLLAVWLLLLTMDA
                     LDLTHFSFLPALLEVGVLVALSGVVLGGLLDYLALRKTHLT"
     gene            complement(1733..2311)
                     /locus_tag="NBI74_000357"
     CDS             complement(1733..2311)
                     /locus_tag="NBI74_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCD1930735.1"
                     /translation="MFKKILFPLVALFMLAGCAKPPTTIEVSPTITLPQQDPSLMGVT
                     VSINGADQRTDQALAKVTRDNQIVTLTASRDLRFLLQEVLEKQMTARGYMVGPNGPVN
                     LQIIVSQLYADVSQGNVRYNIATKADIAIIATAQNGNKMTKNYRASYNVEGAFQASNK
                     NIADAVNSVLTDTIADMSQDTSIHEFIKQNAR"
     gene            2616..2933
                     /gene="bolA"
                     /locus_tag="NBI74_000358"
     CDS             2616..2933
                     /gene="bolA"
                     /locus_tag="NBI74_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414969.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator BolA"
                     /protein_id="HCD1930736.1"
                     /translation="MMIRERIEEKLRAAFQPVFLEVVDESYRHNVPAGSESHFKVVLV
                     SDRFTGERFLNRHRMIYSTLAEELSTTVHALALHTYTIKEWEGLQDTVFASPPCRGAG
                     SIA"
     gene            3277..4575
                     /gene="tig"
                     /locus_tag="NBI74_000359"
     CDS             3277..4575
                     /gene="tig"
                     /locus_tag="NBI74_000359"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001716227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HCD1930737.1"
                     /translation="MQVSVETTQGLGRRVTITIAADSIETAVKSELVNVAKKVRIDGF
                     RKGKVPMNIVAQRYGASVRQDVLGDLMSRNFIDAIIKEKINPAGAPTYVPGEYKLGED
                     FTYSVEFEVYPEVELQGLEAIEVEKPIVEVTDADVDGMLDTLRKQQATWKEKDGAVEA
                     EDRVTIDFTGSVDGEEFEGGKASDFVLAMGQGRMIPGFEDGIKGHKAGEEFTIDVTFP
                     EEYHAENLKGKAAKFAINLKKVEERELPELTEEFIKRFGVEDGSVEGLRAEVRKNMER
                     ELKSAIRNRVKSQAIEGLVKANDIDVPAALIDSEIDVLRRQAAQRFGGNEKQALELPR
                     ELFEEQAKRRVVVGLLLGEVIRTNELKADEERVKGLIEEMASAYEDPKEVIEFYSKNK
                     ELMDNMRNVALEEQAVEAVLAKAKVTEKETTFNELMNQQA"
     gene            4821..5444
                     /gene="clpP"
                     /locus_tag="NBI74_000360"
     CDS             4821..5444
                     /gene="clpP"
                     /locus_tag="NBI74_000360"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006809880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HCD1930738.1"
                     /translation="MSYSGERDNFAPHMALVPMVIEQTSRGERSFDIYSRLLKERVIF
                     LTGQVEDHMANLIVAQMLFLEAENPEKDIYLYINSPGGVITAGMSIYDTMQFIKPDVS
                     TICMGQAASMGAFLLTAGAKGKRFCLPNSRVMIHQPLGGYQGQATDIEIHAREILKVK
                     GRMNELMALHTGQSLEQIERDTERDRFLSAPEAVEYGLVDSILTHRN"
     gene            5570..6844
                     /gene="clpX"
                     /locus_tag="NBI74_000361"
     CDS             5570..6844
                     /gene="clpX"
                     /locus_tag="NBI74_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001396736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATP-binding subunit ClpX"
                     /protein_id="HCD1930739.1"
                     /translation="MTDKRKDGSGKLLYCSFCGKSQHEVRKLIAGPSVYICDECVDLC
                     NDIIREEIKEVAPHRERSALPTPHEIRNHLDDYVIGQEQAKKVLAVAVYNHYKRLRNG
                     DTSNGVELGKSNILLIGPTGSGKTLLAETLARLLDVPFTMADATTLTEAGYVGEDVEN
                     IIQKLLQKCDYDVQKAQRGIVYIDEIDKISRKSDNPSITRDVSGEGVQQALLKLIEGT
                     VAAVPPQGGRKHPQQEFLQVDTSKILFICGGAFAGLDKVIANRVETGSGIGFGATVKA
                     KSDKASEGELLAQVEPEDLIKFGLIPEFIGRLPVVATLNELSEEALIQILKEPKNALT
                     KQYQALFNLEGVDLEFRDEALDAIAKKAMARKTGARGLRSIVEAALLDTMYDLPSMED
                     VEKVVIDESVIDGQSKPLLIYGKPEAQQASGE"
     gene            7032..9386
                     /gene="lon"
                     /locus_tag="NBI74_000362"
     CDS             7032..9386
                     /gene="lon"
                     /locus_tag="NBI74_000362"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001469947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HCD1930740.1"
                     /translation="MNPERSERIEIPVLPLRDVVVYPHMVIPLFVGREKSIRCLEAAM
                     DHDKKIMLVAQKEASTDEPGVNDLFTVGTVASILQMLKLPDGTVKVLVEGLQRARISA
                     LSDNGEHFSAKAEYLESPTIDEREQEVLVRTAISQFEGYIKLNKKIPPEVLTSLNSID
                     DPARLADTIAAHMPLKLADKQSVLEMSDVNERLEYLMAMMESEIDLLQVEKRIRNRVK
                     KQMEKSQREYYLNEQMKAIQKELGEMDDAPDENEALKRKIDAAKMPKEAKEKAEAELQ
                     KLKMMSPMSAEATVVRGYIDWMVQVPWNARSKVKKDLRQAQEILDTDHYGLERVKDRI
                     LEYLAVQSRVNKIKGPILCLVGPPGVGKTSLGQSIAKATGRKYVRMALGGVRDEAEIR
                     GHRRTYIGSMPGKLIQKMAKVGVKNPLFLLDEIDKMSSDMRGDPASALLEVLDPEQNV
                     AFSDHYLEVDYDLSDVMFVATSNSMNIPAPLLDRMEVIRLSGYTEDEKLNIAKRHLLP
                     KQIERNALKKGELTVDDSAIIGIIRYYTREAGVRGLEREISKLCRKAVKQLLLDKSLK
                     HIEINGDNLHDYLGVQRFDYGRADNENRVGQVTGLAWTEVGGDLLTIETACVPGKGKL
                     TYTGSLGEVMQESIQAALTVVRARAEKLGINPDFYEKRDIHVHVPEGATPKDGPSAGI
                     AMCTALVSCLTGNPVRADVAMTGEITLRGQVLPIGGLKEKLLAAHRGGIKTVLIPFEN
                     KRDLEEIPDNVIADLDIHPVKRIEEVLTLALQNEPSGMQVVTAK"
     gene            9595..9867
                     /gene="hupB"
                     /locus_tag="NBI74_000363"
     CDS             9595..9867
                     /gene="hupB"
                     /locus_tag="NBI74_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein HU-beta"
                     /protein_id="HCD1930741.1"
                     /translation="MNKSQLIDKIAAGADISKAAAGRALDAIIASVTESLKEGDDVAL
                     VGFGTFAVKERAARTGRNPQTGKEITIAAAKVPSFRAGKALKDAVN"
     gene            10059..11930
                     /gene="ppiD"
                     /locus_tag="NBI74_000364"
     CDS             10059..11930
                     /gene="ppiD"
                     /locus_tag="NBI74_000364"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HCD1930742.1"
                     /translation="MMDSLRTAANSLVLKIIFGIIIVSFILTGVSGYLIGGGNNYAAK
                     VNDQEISRGQFENAFNSERNRMQQQLGDQYSELAANEGYMKTLRQQVLNRLIDEALLD
                     QYARELKLGISDEQVKQAIFATPAFQVDGKFDNSRYNGILNQMGMTADQYAQALRNQL
                     TTQQLINGVAGTDFMLKGETDELAALVAQQRVVREAAIDVNALAAKQPVTEQEIASYY
                     EQNKNNFMTPEQFRVSYIKLDAATMQQPVSDADIQSYYDQHQDQFTQPQRTRYSIIQT
                     KTEDEAKAVLDELNKGGDFAALAKEKSADIISARNGGDMGWLEDATIPDELKNAGLKE
                     KGQLSGVIKSSVGFLIVRLDDIQPAKVKSLDEVRDDIAAKVKHEKALDAYYALQQKVS
                     DAASNDTESLAGAEQAAGVKATQTGWFSKDNLPEELNFKPVADAIFNGGLVGENGAPG
                     INSDIITVDGDRAFVLRISEHKPEAVKPLADVQEQVKALVQHNKAEQQAKVDAEKLLV
                     DLKAGKGAEAMQAAGLKFGEPKTLSRSGRDPISQAAFALPLPAKDKPSYGMATDMQGN
                     VVLLALDEVKQGSMPEDQKKAMVQGITQNNAQIVFEALMSNLRKEAKIKIGDALEQQ"
     gene            12081..12452
                     /locus_tag="NBI74_000365"
     CDS             12081..12452
                     /locus_tag="NBI74_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HCD1930743.1"
                     /translation="MKHGIKALLITLSLACAGMSHSALAAAPAAKPTTVETKAEAPAA
                     QSKAAVPAKASDEEGTRVSINNASAEELARAMNGVGLKKAQAIVSYREEYGPFKTVED
                     LKQVPGMGNSLVERNMAVLTL"
     gene            12558..12956
                     /gene="fadM"
                     /locus_tag="NBI74_000366"
     CDS             12558..12956
                     /gene="fadM"
                     /locus_tag="NBI74_000366"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain acyl-CoA thioesterase FadM"
                     /protein_id="HCD1930744.1"
                     /translation="MQTQIKVRGYHLDVYQHVNNARYLEFLEEARWHGLENSDSFHWM
                     TAHNIAFVVVNININYRRPAVLSDLLTITSQLQQLNGKSGILSQVITLEPEGQVVADA
                     LITFVCIDLKTQKALALEGELREKLEQMVK"
     gene            complement(13008..13703)
                     /gene="queC"
                     /locus_tag="NBI74_000367"
     CDS             complement(13008..13703)
                     /gene="queC"
                     /locus_tag="NBI74_000367"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HCD1930745.1"
                     /translation="MKRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEID
                     VARELALKLGARAHKVLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNIL
                     FLTLAAIYAYQVKAEAVITGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPL
                     MWIDKAETWALADYYGKLDLVRNETLTCYNGIKGDGCGHCAACNLRANGLNHYLADKP
                     TVMVAMKQKTGLK"
     gene            complement(13768..15468)
                     /locus_tag="NBI74_000368"
     CDS             complement(13768..15468)
                     /locus_tag="NBI74_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SgrR family transcriptional regulator"
                     /protein_id="HCD1930746.1"
                     /translation="MRLLNRLNQYQRLWQPSAGKPQTVTVSELAERCFCSERHVRTVL
                     RQAQEAGWLEWQAQSGRGKRGQLRFLVTPESLRNAMMEQALETGKQQDVLELAQLAPG
                     ELRTLLQPFMGGQWQNDTPTLRIPYYRPLEPLQPGFLPGRAEQHLAGQIFSGLTRFDN
                     NTQRPIGDLAHHWETSTDGLRWDFYLRSTLHWHNGDAVKASHLHQRLLMLLQLPALDQ
                     LFISVKRIEVTHPQCLTFFLHRPDYWLAHRLASYCSHLAHPQFPLIGTGPFRLTQFTA
                     ELVRLESHDYYHLRHPLLKAVEYWITPPLFEKDLGTSCRHPVQITIGKPEELQRVSQV
                     SSGISLGFCYLTLRKSLRLSLWQARKVISIIHQSGLLQTLEVGENLITASHALLPGWT
                     IPHWQVPDEVKLPKTLTLVYHLPIELHTMAERLQATLAAEGCELTIIFHNAKNWDDTT
                     LLAHADLMMGDRLIGEAPEYTLEQWLRCDPLWPHVFDAPAYAHLQSTLDAVQVMPDEE
                     NRFNALKAVFSQLMADATLTPLFNYHYRISAPPGVNGVRLTPRGWFEFTEAWLPAPSQ
                     "
     gene            15568..16386
                     /gene="cof"
                     /locus_tag="NBI74_000369"
     CDS             15568..16386
                     /gene="cof"
                     /locus_tag="NBI74_000369"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HMP-PP phosphatase"
                     /protein_id="HCD1930747.1"
                     /translation="MARLAAFDMDGTLLMPDHHLGEKTLSTLARLRERDITLTFATGR
                     HALEMQHILGALSLDAYLITGNGTRVHSLEGELLHRDDLPADVAELVLYQQWDTRASM
                     HIFNDDGWFTGKEIPALLQAFVYSGFRYQIIDVKKMPLGSVTKICFCGDHNDLTRLQI
                     QLYEALGERAHLCFSATDCLEVLPVGCNKGAALTVLTQHLGLSLRDCMAFGDAMNDRE
                     MLGSVGSGFIMGNAMPQLRAELPHLPVIGHCRNQAVSHYLTHWLDYPHLPYSPE"
     gene            16539..16997
                     /gene="decR"
                     /locus_tag="NBI74_000370"
     CDS             16539..16997
                     /gene="decR"
                     /locus_tag="NBI74_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator DecR"
                     /protein_id="HCD1930748.1"
                     /translation="MLDKIDRKLLALLQQDCTLSLQALAEAVNLTTTPCWKRLKRLED
                     DGILIGKVALLDPEKIGLGLTAFVLIKTQHHSSEWYCRFVTVVTEMPEVLGFWRMAGE
                     YDYLMRVQVADMKRYDEFYKRLVNSVPGLSDVTSSFAMEQIKYTTSLPIE"
     gene            17027..18799
                     /locus_tag="NBI74_000371"
     CDS             17027..18799
                     /locus_tag="NBI74_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SmdA family multidrug ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HCD1930749.1"
                     /translation="MRLFAQLSWYFRREWRRYLGAVALLVIIAMLQLVPPKVVGIVVD
                     GVTEQHFTTGQILMWIATMVLIAVVVYLLRYVWRVLLFGASYQLAVELREDYYRQLSR
                     QHPEFYLRHRTGDLMARATNDVDRVVFAAGEGVLTLVDSLVMGCAVLIMMSTQISWQL
                     TLFALLPMPVMAIMIKRNGDALHERFKLAQAAFSSLNDRTQESLTSIRMIKAFGLEDR
                     QSALFAADAEDTGKKNMRVARIDARFDPTIYIAIGMANLLAIGGGSWMVVQGSLTLGQ
                     LTSFMMYLGLMIWPMLALAWMFNIVERGSAAYSRIRTMLAEAPVVIDGSDKVPEGRGE
                     LDVNIRQFTYPQTDHPALENVNFALKPGQMVGICGPTGSGKSTLLSLIQRHFDVSEGD
                     IRFHDIPLTKLQLDSWRSRLAVVSQTPFLFSDTVANNIALGCPNATQQEIEHVARLAS
                     VHDDILRLPQGYDTEVGERGVMLSGGQKQRISIARALLVNAEILILDDALSAVDGRTE
                     HQILHNLRQWGQGRTVIISAHRLSALTEASEIIVMQHGHIAQRGNHDVLVQQSGWYRD
                     MYRYQQLEAALDDAPEIREEAIDA"
     gene            18792..20573
                     /locus_tag="NBI74_000372"
     CDS             18792..20573
                     /locus_tag="NBI74_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SmdB family multidrug efflux ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HCD1930750.1"
                     /translation="MRSFSQLWPTLKRLLAYGSPWRKPLGIAVLMMWVAAAAEVSGPL
                     LISYFIDNMVAKNNLPLKVVAGLAAAYVGLQLFAAGLHYAQSLLFNRAAVGVVQQLRT
                     DVMDAALRQPLSEFDTQPVGQVISRVTNDTEVIRDLYVTVVATVLRSAALVGAMLVAM
                     FSLDWRMALVAIMIFPVVLVVMVIYQRYSTPIVRRVRAYLADINDGFNEIINGMSVIQ
                     QFRQQARFGERMGEASRSHYMARMQTLRLDGFLLRPLLSLFSSLILCGLLMLFGFSAS
                     GTIEVGVLYAFISYLGRLNEPLIELTTQQAMLQQAVVAGERVFELMDGPRQQYGNDDR
                     PLQSGTIEVDNVSFAYRDDNLVLKNINLSVPSRNFVALVGHTGSGKSTLASLLMGYYP
                     LTEGEIRLDGRPLSTLSHSALRQGVAMVQQDPVVLADTFLANVTLGRDISEERVWQAL
                     ETVQLAELARSMSDGIYTLLGEQGNNLSVGQKQLLALARVLVETPQILILDEATASID
                     SGTEQAIQHALAAVREHTTLVVIAHRLSTIVDADTILVLHRGQAVEQGTHQQLLAAQG
                     RYWQMYQLQLAGEELAASVREEESLSA"
     gene            20754..21092
                     /gene="glnK"
                     /locus_tag="NBI74_000373"
     CDS             20754..21092
                     /gene="glnK"
                     /locus_tag="NBI74_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HCD1930751.1"
                     /translation="MKLVTVIIKPFKLEDVREALSSIGIQGLTVTEVKGFGRQKGHAE
                     LYRGAEYSVNFLPKVKIDVAIADDQLDEVIDIVSKAAYTGKIGDGKIFVAELQRVIRI
                     RTGEADEAAL"
     gene            21122..22408
                     /gene="amtB"
                     /locus_tag="NBI74_000374"
     CDS             21122..22408
                     /gene="amtB"
                     /locus_tag="NBI74_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter AmtB"
                     /protein_id="HCD1930752.1"
                     /translation="MKIATIKTGLTSLAMLPGLVMAAPAVADKADNAFMMICTALVLF
                     MTIPGIALFYGGLIRGKNVLSMLTQVTVTFALVCILWVVYGYSLAFGEGNNFFGNING
                     LMLKNIELTAVMGSIYQYIHVAFQGSFACITVGLIVGALAERIRFSAVLIFVVVWLTL
                     SYIPIAHMVWGGGLLASHGALDFAGGTVVHINAAIAGLVGAYLIGKRVGFGKEAFKPH
                     NLPMVFTGTAILYIGWFGFNAGSAGTANEIAALAFVNTVVATAAAILGWIFGEWALRG
                     KPSLLGACSGAIAGLVGVTPACGYIGVGGALIIGVVAGLAGLWGVTMLKRLLRVDDPC
                     DVFGVHGVCGIVGCIMTGIFAASSLGGVGFAEGVTMGHQLLVQLESIAITIVWSGVVA
                     FIGYKLADLTVGLRVPEEQEREGLDVNSHGENAYNA"
     gene            complement(22457..23317)
                     /gene="tesB"
                     /locus_tag="NBI74_000375"
     CDS             complement(22457..23317)
                     /gene="tesB"
                     /locus_tag="NBI74_000375"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase II"
                     /protein_id="HCD1930753.1"
                     /translation="MSQALKNLLTLLNLEKIEEGLFRGQSEDLGLRQVFGGQVVGQAL
                     YAAKETVPDERLVHSFHSYFLRPGDSKKPIIYDVETLRDGNSFSARRVSAIQNGKPIF
                     YMTASFQAPEAGFEHQKTMPSAPAPDGLPSETQIAQSLAHLLPPVLKDKFICDRPLEV
                     RPVEFHNPLKGHVAEPHRQVWIRANGSVPDDLRVHQYLLGYASDLNFLPVALQPHGIG
                     FLEPGIQIATIDHSMWFHRPFNLNEWLLYSVESTSASSARGFVRGEFYTQDGVLVAST
                     VQEGVMRNHN"
     gene            23535..24107
                     /locus_tag="NBI74_000376"
     CDS             23535..24107
                     /locus_tag="NBI74_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaY family lipoprotein"
                     /protein_id="HCD1930754.1"
                     /translation="MKLVHMASGLAVAIALAACADKSADIQTPAPAANTSISATQQPA
                     IQQPNVSGTVWIRQKVALPPDAVLTVTLSDASLADAPSKVLAQKAVRTEGKQSPFSFV
                     LPFNPVDVQPNARILLSAAITVNDKLVFITDTVQPVINQGGTKADLTLVPVQQTAVPV
                     QASGGATTTVPSTSPTQVNPSSAVPAPTQY"
     gene            complement(24138..24449)
                     /locus_tag="NBI74_000377"
     CDS             complement(24138..24449)
                     /locus_tag="NBI74_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HCD1930755.1"
                     /translation="MEKEDSFPQRVWQIVAAIPEGYVTTYGDVAKLAGSPRAARQVGG
                     VLKRLPEGSTLPWHRVVNRHGTISLTGPDLQRQRQALLAEGVMVSGSGQIDLQLYRWN
                     Y"
     gene            24612..24708
                     /gene="ffs"
                     /locus_tag="NBI74_000378"
     ncRNA           24612..24708
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="NBI74_000378"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            24828..25181
                     /locus_tag="NBI74_000379"
     CDS             24828..25181
                     /locus_tag="NBI74_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1428 family protein"
                     /protein_id="HCD1930756.1"
                     /translation="MKYVDGFVVAVPADKKDAYREMAAKVAPLFKEFGALRIVECWAS
                     DVPDGKVTDFRMAVKAEENEEVVFSWIEYPSKEVRDAANQKMMSDPRMKEFGESMPFD
                     GKRMIYGGFESIIDE"
     gene            complement(25223..26773)
                     /gene="pdeB"
                     /locus_tag="NBI74_000380"
     CDS             complement(25223..26773)
                     /gene="pdeB"
                     /locus_tag="NBI74_000380"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414990.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeB"
                     /protein_id="HCD1930757.1"
                     /translation="MRTRHLVGLISGVLILSVLLPVGLSIWLAHQQVETSFIEELNTY
                     SSRVAIRANKVATQGKDALQELERWQGAACSEAHLMEMRRVSYSYRYIQEVVYIDNNV
                     PQCSSLEHESPPDTFPEPGKISKDGYRVWLTSHNDLGIIRYMVAMGTAHYVVMIDPAS
                     FIDVIPYSSWQIDAAIIGNAHNVVITSSDELAQGIITRLQKTPGEHIENNGIIYDILP
                     LPEMNISIITWASTKMLQKGWHRQVFIWLPLGLVIGLLAAMFVLRILRRIQSPHHRLQ
                     DAIENRDICVHYQPIVSLANGKIVGAEALARWPQTDGSWLSPDSFIPLAQQTGLSEPL
                     TLLIIRSVFEDMGDWLRQHSQQNISINLESTVLTSEKIPQLLREMINHYQVNPRQIAL
                     ELTEREFADPKTSAPIISRYRETGHEIYLDDFGTGYSSLSYLQDLDVDILKIDKSFVD
                     ALEYKNVTPHIIEMAKTLKLKMVAEGIETSKQEEWLRQHGVHYGQGWLYSKALPKEDF
                     LRWAEQHL"
     gene            complement(26937..27407)
                     /locus_tag="NBI74_000381"
     CDS             complement(26937..27407)
                     /locus_tag="NBI74_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaC family protein"
                     /protein_id="HCD1930758.1"
                     /translation="MTEIQRLLTETIESLNTREKRDNKPRFSISFIRKHPGLFIGMYV
                     AFFATLAVMLQSETLSGSVWLLVVLFILLNGFFFFDVYPRYRYEDIDVLDFRVCYNGE
                     WYNTRFVPAALVEAILNSPRVADVHKEQLQKMIVRKGELSFYDIFTLARAESTS"
     gene            complement(27523..28074)
                     /gene="maa"
                     /locus_tag="NBI74_000382"
     CDS             complement(27523..28074)
                     /gene="maa"
                     /locus_tag="NBI74_000382"
                     /EC_number="2.3.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose O-acetyltransferase"
                     /protein_id="HCD1930759.1"
                     /translation="MSTEKEKMIAGELYHSADEALSRDRLRARRLIHRYNHSLAEEHT
                     LRQQILADLFGQVTEAYIEPTFRCDYGYNIFLGNNFFANFDCVMLDVCPIRIGDNCML
                     APGVHIYTATHPIDPVARNSGAELGKPVTIGNNVWIGGRAVINPGVTIGDNVVVASGA
                     VVTKDVPDNVVVGGNPARIIKKL"
     gene            complement(28247..28465)
                     /gene="hha"
                     /locus_tag="NBI74_000383"
     CDS             complement(28247..28465)
                     /gene="hha"
                     /locus_tag="NBI74_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin expression modulator Hha"
                     /protein_id="HCD1930760.1"
                     /translation="MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFY
                     SAADHRLAELTMNKLYDKIPSSVWKFIR"
     gene            complement(28491..28865)
                     /gene="tomB"
                     /locus_tag="NBI74_000384"
     CDS             complement(28491..28865)
                     /gene="tomB"
                     /locus_tag="NBI74_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hha toxicity modulator TomB"
                     /protein_id="HCD1930761.1"
                     /translation="MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAI
                     NLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYLDDTFMLFSSYGINMQDLQKWRK
                     SGNRLFRCFVNATKENPASLSC"
     gene            complement(29411..32560)
                     /locus_tag="NBI74_000385"
     CDS             complement(29411..32560)
                     /locus_tag="NBI74_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit"
                     /protein_id="HCD1930762.1"
                     /translation="MPNFFIDRPIFAWVIAIIIMLAGGLAILKLPVAQYPTIAPPAVT
                     ISASYPGADAKTVQDTVTQVIEQNMNGIDNLMYMSSNSDSTGTVQITLTFESGTDADI
                     AQVQVQNKLQLAMPLLPQEVQQQGVSVEKSSSSFLMVVGVINTDGTMTQEDISDYVAA
                     NMKDAISRTSGVGDVQLFGSQYAMRIWMNPNELNKFQLTPVDVITAIKAQNAQVAAGQ
                     LGGTPPVKGQQLNASIIAQTRLTSTEEFGKILLKVNQDGSRVLLRDVAKIELGGENYD
                     IIAEFNGQPASGLGIKLATGANALDTAAAIRAELAKMEPFFPSGLKIVYPYDTTPFVK
                     ISIHEVVKTLVEAIILVFLVMYLFLQNFRATLIPTIAVPVVLLGTFAVLAAFGFSINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVMAEEGLPPKEATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGSTGAIYRQFSITIVSAMALSVLVALILTPALCATMLKPIAKGDHGEG
                     KKGFFGWFNRMFEKSTHHYTDSVGGILRSTGRYLVLYLIIVVGMAYLFVRLPSSFLPD
                     EDQGVFMTMVQLPAGATQERTQKVLNEVTNYYLTKEKNNVESVFAVNGFGFAGRGQNT
                     GIAFVSLKDWADRPGEENKVEAITMRATRAFSQIKDAMVFAFNLPAIVELGTATGFDF
                     ELIDQAGLGHEKLTQARNQLLAEAAKHPDMLTSVRPNGLEDTPQFKIDIDQEKAQALG
                     VSINDINTTLGAAWGGSYVNDFIDRGRVKKVYVMSEAKYRMLPDDIGDWYVRAADGQM
                     VPFSAFSSSRWEYGSPRLERYNGLPSMEILGQAAPGKSTGEAMELMEQLASKLPTGVG
                     YDWTGMSYQERLSGNQAPSLYAISLIVVFLCLAALYESWSIPFSVMLVVPLGVIGALL
                     AATFRGLTNDVYFQVGLLTTIGLSAKNAILIVEFAKDLMDKEGKGLIEATLDAVRMRL
                     RPILMTSLAFILGVMPLVISTGAGSGAQNAVGTGVMGGMVTATVLAIFFVPVFFVVVR
                     RRFSRKNEDIEHNHTVDHH"
     gene            complement(32583..33776)
                     /gene="acrA"
                     /locus_tag="NBI74_000386"
     CDS             complement(32583..33776)
                     /gene="acrA"
                     /locus_tag="NBI74_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrA"
                     /protein_id="HCD1930763.1"
                     /translation="MNKNRGFTPLAVVLMLSGSLALTGCDDKQAQQGGQQMPAVGVVT
                     VKTEPLQITTELPGRTSAYRIAEVRPQVSGIILKRNFKEGSDIEAGVSLYQIDPATYQ
                     AAYDSAKGDLAKAQAAANIAQLTVNRYQKLLGTQYISKQEYDQALADAQQANAAVTAA
                     KAAVETARINLAYTKVTSPISGRIGKSNVTEGALVQNGQATALATVQQLDPIYVDVTQ
                     SSNDFLRLKQELANGTLKQENGKAKVSLITSDGIKFPQDGTLEFSDVTVDQTTGSITL
                     RAIFPNPDHTLLPGMFVRARLEEGLNPNAILVPQQGVTRTPRGDATVLVVGADDKVET
                     RPIVASQAIGDKWLVTEGLKAGDRVVISGLQKVRPGVQVKAQEVTADNNQQAASGAQP
                     EQSKS"
     gene            33918..34565
                     /gene="acrR"
                     /locus_tag="NBI74_000387"
     CDS             33918..34565
                     /gene="acrR"
                     /locus_tag="NBI74_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrR"
                     /protein_id="HCD1930764.1"
                     /translation="MARKTKQEAQETRQHILDVALRLFSQQGVSSTSLGEIAKAAGVT
                     RGAIYWHFKDKSDLFSEIWELSESNIGELELEYQAKFPGDPLSVLREILIHVLESTVT
                     EERRRLLMEIIFHKCEFVGEMAVVQQAQRNLCLESYDRIEQTLKHCIEAKMLPADLMT
                     RRAAIIMRGYISGLMENWLFAPQSFDLKKEARDYVAILLEMYLLCPTLRNPATNE"
     gene            34693..38055
                     /gene="mscK"
                     /locus_tag="NBI74_000388"
     CDS             34693..38055
                     /gene="mscK"
                     /locus_tag="NBI74_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel MscK"
                     /protein_id="HCD1930765.1"
                     /translation="MTMFQYYKRSRHFVFSAFIAFVFVLLCQNTAFARASSNGDLPTK
                     ADLQAQLDSLNKQKDLSAQDKLVQQDLTDTLATLDKIDRVKEETVQLRQKVAEAPEKM
                     RQATAALTALSDVDNDEETRKILSALSLRQLETRVAQALDDLQNAQNDLASYNSQLVS
                     LQTQPERVQNAMYNASQQLQQIRSRLDGTDVGETALRPSQKVLMQVQQALLNAEIDQQ
                     RKSLEGNTVLQDTLQKQRDYVTANSARLEHQLQLLQEAVNSKRLTLTEKTAQEAVSPD
                     EAARIQANPLVKQELEINQQLSQRLITATQNGNQLMQQNIKVKNWLERALQSERNIKE
                     QIAVLKGSLLLSRILYQQQQTLPSADELENMTNRIADLRLEQFEVNQQRDALFQSDAF
                     VSKLEEGHTNEVNSEVHDALLQVVDMRRELLDQLNKQLGNQLMMAINLQINQQQLMSV
                     SKNLKSILTQQIFWVNSNRPMDWDWIKAFPQTLKDEFKSMKITVNWEKAWPAVFIAFL
                     AGLPLLLIAGLIHWRLGWLKAYQQKLASAVGSLRNDSQLNTPKAILIDLIRALPVCLI
                     ILAVGLILLTMQLNISELLWSFSKKLAIFWLVFGLCWKVLEKNGVAVRHFGMPEQQTS
                     HWRRQIVRISLALLPIHFWSVVAELSPLHLMDDVLGQAMIFFNLLLIAFLVWPMCRES
                     WRDKESHTMRLVTITVLSIIPIALMVLTATGYFYTTLRLSGRWIETVYLVIIWNLLYQ
                     TVLRGLSVAARRIAWRRALARRQNLVKEGAEGAEPPEEPTIALEQVNQQTLRITMLLM
                     FALFGVMFWAIWSDLITVFSYLDSITLWHYNATEAGAAVVKNVTMGSLLFAIIASMVA
                     WALIRNLPGLLEVLVLSRLNMRQGASYAITTILNYIIIAVGAMTVFGSLGVSWDKLQW
                     LAAALSVGLGFGLQEIFGNFVSGLIILFERPVRIGDTVTIGSFSGTVSKIRIRATTIT
                     DFDRKEVIIPNKAFVTERLINWSLTDTTTRLVIRLGVAYGSDLEKVRKVLLKAATEHP
                     RVMHEPMPEVFFTAFGASTLDHELRLYVRELRDRSRTVDELNRTIDQLCRENDINIAF
                     NQLEVHLHNEKGDEVTEVKRDYKGDDPTPAVG"
     gene            complement(38094..38258)
                     /gene="rsmS"
                     /locus_tag="NBI74_000389"
     CDS             complement(38094..38258)
                     /gene="rsmS"
                     /locus_tag="NBI74_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pleiotropic regulatory protein RsmS"
                     /protein_id="HCD1930766.1"
                     /translation="MSLENAPDDVKLAVDLIVLLEENQIPARTVLRALDIVKRDYEKK
                     LQSDETSQSE"
     gene            complement(38272..38799)
                     /gene="priC"
                     /locus_tag="NBI74_000390"
     CDS             complement(38272..38799)
                     /gene="priC"
                     /locus_tag="NBI74_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N''"
                     /protein_id="HCD1930767.1"
                     /translation="MKTALLLEKLEGQLATLRQRCAPVAQFATLSARFDRHLFQTRAT
                     TLQACLDESGDNLAALRHAVEQQQLPQVAWLAEHLAAQLEAIAREATAWSLREWDSAP
                     PKIARWQRKRIQHQDFERRLREMVAERRARLARATDLVEQQTLHREVEAYEARLARCR
                     HALEKIENRLARLTR"
     gene            38869..39246
                     /locus_tag="NBI74_000391"
     CDS             38869..39246
                     /locus_tag="NBI74_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF454 family protein"
                     /protein_id="HCD1930768.1"
                     /translation="MQRIILIIIGWLAVVLGTLGVVLPVLPTTPFILLAAWCFARSSP
                     RFHAWLLYRSWFGSYLRFWQKHHAMPRGAKPRAILLILITFTISLWFVQMPWVRIMLL
                     VILACLLFYMWRIPVIDEKQEKH"
     gene            39399..39950
                     /gene="apt"
                     /locus_tag="NBI74_000392"
     CDS             39399..39950
                     /gene="apt"
                     /locus_tag="NBI74_000392"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HCD1930769.1"
                     /translation="MTATAQQLEYLKNSIKSIQDYPKPGILFRDVTSLLEDPKAYALS
                     IDLLVERYKNAGITKVVGTEARGFLFGAPVALGLGVGFVPVRKPGKLPRETISETYDL
                     EYGTDQLEIHVDAIKPGDKVLVVDDLLATGGTIEATVKLIRRLGGEVADAAFIINLFD
                     LGGEQRLEKQGITSYSLVPFPGH"
     gene            40079..42010
                     /gene="dnaX"
                     /locus_tag="NBI74_000393"
     CDS             40079..42010
                     /gene="dnaX"
                     /locus_tag="NBI74_000393"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HCD1930770.1"
                     /translation="MSYQVLARKWRPQTFADVVGQEHVLTALANGLSLGRIHHAYLFS
                     GTRGVGKTSIARLLAKGLNCETGITATPCGVCDNCREIEQGRFVDLIEIDAASRTKVE
                     DTRDLLDNVQYAPARGRFKVYLIDEVHMLSRHSFNALLKTLEEPPEHVKFLLATTDPQ
                     KLPVTILSRCLQFHLKALDVEQIRHQLEHILNEEHIAHEPRALQLLARAAEGSLRDAL
                     SLTDQAIASGDGQVSTQGVSAMLGTLDDDQALSLVEAMVEANGERVMALINEAAARGI
                     EWEALLVEMLGLLHRIAMVQLSPAALGNDMAAIELRMRELARTIPPTDIQLYYQTLLI
                     GRKELPYAPDRRMGVEMTLLRALAFHPRMPLPDPEVPRQSFAPVAPTAVMTPTQVPPQ
                     PQSAPQQAPTVPLPETTSQVLAARQQLQRVQGATKAKKSEPAAATRARPVNNAALERL
                     ASVTDRVQARPVPSALEKAPAKKEAYRWKATTPVMQQKEVVATPKALKKALEHEKTPE
                     LAAKLAAEAIERDPWAAQVSQLSLPKLVEQVALNAWKEESDNAVCLHLRSSQRHLNNR
                     GAQQKLAEALSTFKGSTVELTIVEDDNPAVRTPLEWRQAIYEEKLAQARESIIADNNI
                     QTLRRFFDAELDEESIRPI"
     misc_feature    41343..41407
                     /gene="dnaX"
                     /locus_tag="NBI74_000393"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00382"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="DnaX frameshifting element; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00382"
     gene            42063..42392
                     /locus_tag="NBI74_000394"
     CDS             42063..42392
                     /locus_tag="NBI74_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001364632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HCD1930771.1"
                     /translation="MFGKGGLGNLMKQAQQMQEKMQKMQEEIAQLEVTGESGAGLVKV
                     TINGAHNCRRVEIDPSLLEDDKEMLEDLVAAAFNDAARRIEETQKEKMASVSSGMQLP
                     PGFKMPF"
     gene            42392..42997
                     /gene="recR"
                     /locus_tag="NBI74_000395"
     CDS             42392..42997
                     /gene="recR"
                     /locus_tag="NBI74_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HCD1930772.1"
                     /translation="MQTSPLLTQLMEALRCLPGVGPKSAQRMAFTLLQRDRSGGMRLA
                     QALTRAMSEIGHCADCRTFTEQEVCNICSNPRRQENGQICVVESPADIYAIEQTGQFS
                     GRYFVLMGHLSPLDGIGPDDIGLDRLEQRLAEEKITEVILATNPTVEGEATANYIAEL
                     CAQYDVEASRIAHGVPVGGELEMVDGTTLSHSLAGRHKIRF"
     gene            43107..44981
                     /gene="htpG"
                     /locus_tag="NBI74_000396"
     CDS             43107..44981
                     /gene="htpG"
                     /locus_tag="NBI74_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003903109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HCD1930773.1"
                     /translation="MKGQETRGFQSEVKQLLHLMIHSLYSNKEIFLRELISNASDAAD
                     KLRFRALSNPDLYEGDGELRVRVSFDKDKRTLTISDNGVGMTRDEVIDHLGTIAKSGT
                     KSFLESLGSDQAKDSQLIGQFGVGFYSAFIVADKVTVRTRAAGEKPENGVFWESAGEG
                     EYTVADITKEDRGTEITLHLREGEDEFLDDWRVRSIISKYSDHIALPVEIEKREEKDG
                     ETIISWEKINKAQALWTRNKSEITDEEYKEFYKHIAHDFNDPLTWSHNRVEGKQEYTS
                     LLYIPSQAPWDMWNRDHKHGLKLYVQRVFIMDDAEQFMPNYLRFVRGLIDSSDLPLNV
                     SREILQDSTVTRNLRNALTKRVLQMLEKLAKDDAEKYQTFWQQFGLVLKEGPAEDFAN
                     QEAIAKLLRFASTHTDSSAQTVSLEDYVSRMKEGQEKIYYITADSYAAAKSSPHLELL
                     RKKGIEVLLLSDRIDEWMMNYLTEFDGKPFQSVSKVDESLEKLADEVDESAKEAEKAL
                     TPFIDRVKALLGERVKDVRLTHRLTDTPAIVSTDADEMSTQMAKLFAAAGQKVPEVKY
                     IFELNPDHVLVKRAADTEDEAKFSEWVELLLDQALLAERGTLEDPNLFIRRMNQLLVS
                     "
     gene            45162..45806
                     /gene="adk"
                     /locus_tag="NBI74_000397"
     CDS             45162..45806
                     /gene="adk"
                     /locus_tag="NBI74_000397"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007706188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HCD1930774.1"
                     /translation="MRIILLGAPGAGKGTQAQFIMEKYGIPQISTGDMLRAAVKSGSE
                     LGKQAKDIMDAGKLVTDELVIALVKERIAQEDCRNGFLLDGFPRTIPQADAMKEAGIN
                     VDYVLEFDVPDELIVDRIVGRRVHAPSGRVYHVKFNPPKVEGKDDVTGEELTTRKDDQ
                     EETVRKRLVEYHQMTAPLIGYYSKEAEAGNTKYAKVDGTKPVAEVRADLEKILG"
     gene            45938..46900
                     /gene="hemH"
                     /locus_tag="NBI74_000398"
     CDS             45938..46900
                     /gene="hemH"
                     /locus_tag="NBI74_000398"
                     /EC_number="4.99.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HCD1930775.1"
                     /translation="MRQTKTGILLANLGTPDAPTPEAVKRYLKQFLSDRRVVDTSRLL
                     WWPLLRGVILPLRSPRVAKLYASVWMEGGSPLMVYSRQQQQALAQRLPETPVALGMSY
                     GSPSLESAVDELLAEHVDHIVVLPLYPQYSCSTVGAVWDELARILARKRSIPGISFIR
                     DYADNHDYINALANSVRASFAKHGEPDLLLLSYHGIPQRYADEGDDYPQRCRTTTREL
                     ASALEMAPEKVMMTFQSRFGREPWLMPYTDETLKMLGEKGVGHIQVMCPGFAADCLET
                     LEEIAEQNREVFLGAGGKKYEYIPALNATPEHIEMMANLVAAYR"
     gene            complement(46897..47856)
                     /gene="aes"
                     /locus_tag="NBI74_000399"
     CDS             complement(46897..47856)
                     /gene="aes"
                     /locus_tag="NBI74_000399"
                     /EC_number="3.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl esterase"
                     /protein_id="HCD1930776.1"
                     /translation="MKPENKLPVLDLISAEMKTVVNTLQPDLPPWPATGAIAEQRQYY
                     TLERRFWNADAPEMATRAYRVPTKYGQVKTRIFYPQPDSPATLFYLHGGGFILGNLDT
                     HDRIMRLLASYSQCTVIGIDYTLSPEARFPQAIEEIVAACCYFHQQAEDYQINMSRIG
                     FAGDSAGAMLALASALWLRDKQIDCGKVAGVLLWYGLYGLRDSVARRLLGGVWDGLTQ
                     QDLQMYEEAYLSNDADRESPYYCLFNNDLTREVPPCFIAGAEFDPLLDDSHLLYQTLA
                     AHQQPCEFKLYPGTLHAFLHYSRMMKTADEALRDGAQFFTAQL"
     gene            48008..49312
                     /gene="gsk"
                     /locus_tag="NBI74_000400"
     CDS             48008..49312
                     /gene="gsk"
                     /locus_tag="NBI74_000400"
                     /EC_number="2.7.1.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/guanosine kinase"
                     /protein_id="HCD1930777.1"
                     /translation="MKFPGKRKSKHYFPVNARDPLLQQFQPENETSAAWVVGIDQTLV
                     DIEAKVDDEFIERYGLSAGHSLVIEDDVAEALYQELKQKNLITHQFAGGTIGNTMHNY
                     SVLADDRSVLLGVMCSNIEIGSYAYRYLCNTSSRTDLNYLQGVDGPIGRCFTLIGESG
                     ERTFAISPGHMNQLRAESIPEDVIAGASALVLTSYLVRCKPGEPMPEATMKAIEYAKK
                     YNVPVVLTLGTKFVIAENPQWWQQFLKDHVSILAMNEDEAEALTGESDPLLASDKALD
                     WVDLVLCTAGPIGLYMAGFTEDEAKRKTQHPLLPGAIAEFNQYEFSRAMRHKDCQNPL
                     RVYSHIAPYMGGPEKIMNTNGAGDGALAALLHDITANSYHRSNVPNSSKHKFTWLTYS
                     SLAQVCKYANRVSYQVLNQHSPRLTRGLPEREDSLEESYWDR"
     gene            complement(49442..51118)
                     /locus_tag="NBI74_000401"
     CDS             complement(49442..51118)
                     /locus_tag="NBI74_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001582195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kef family K(+) transporter"
                     /protein_id="HCD1930778.1"
                     /translation="MHHATPLITTIVGGLVLAFILGMLANKLRISPLVGYLLAGVLAG
                     PFTPGFVADTKLAPELAELGVILLMFGVGLHFSLKDLMAVKSIAIPGAIAQIAVATLL
                     GMALSAVLGWSLMTGIVFGLCLSTASTVVLLRALEERQLIDSQRGQIAIGWLIVEDLV
                     MVLTLVLLPAVAGMMEQGDVGFATLAVDMGITIGKVIAFIAIMMLVGRRLVPWIMARS
                     AATGSRELFTLSVLALALGIAFGAVELFDVSFALGAFFAGMVLNESELSHRAAHDTLP
                     LRDAFAVLFFVSVGMLFDPLILIQQPLAVLATLAIILFGKSVAALFLVRLFGHSQRTA
                     LTIAASLAQIGEFAFILAGLGMALNLLPQAGQNLVLAGAILSIMLNPVLFALLEKYLA
                     KTETLEEQTLEEAIEEEKQIPVDICNHALLVGYGRVGSLLGEKLLASDIPLVVIETSR
                     TRVDELRERGVRAVLGNAANEEIMQLAHLECAKWLILTIPNGYEAGEIVASARAKNPD
                     IEIIARAHYDDEVAYITERGANQVVMGEREIARTMLELLETPPAGEVVTR"
     gene            complement(51357..52577)
                     /gene="fsr"
                     /locus_tag="NBI74_000402"
     CDS             complement(51357..52577)
                     /gene="fsr"
                     /locus_tag="NBI74_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fosmidomycin MFS transporter"
                     /protein_id="HCD1930779.1"
                     /translation="MAMSEQTQPVAGAAASTTKARTSFGILGAISLSHLLNDMIQSLI
                     LAIYPLLQSEFSLTFMQIGMITLTFQLASSLLQPVVGYWTDKYPMPWSLPIGMCFTLS
                     GLVLLALAGSFGAVLLAAALVGTGSSVFHPESSRVARMASGGRHGLAQSIFQVGGNFG
                     SSLGPLLAAVIIAPYGKGNVAWFVLAALLAIVVLAQISRWYSAQHRMNKGKPKATIIN
                     PLPRNKVVLAVSILLILIFSKYFYMASISSYYTFYLMQKFGLSIQNAQLHLFAFLFAV
                     AAGTVIGGPVGDKIGRKYVIWGSILGVAPFTLILPYASLHWTGVLTVIIGFILASAFS
                     AILVYAQELLPGRIGMVSGLFFGFAFGMGGLGAAVLGLIADHTSIELVYKICAFLPLL
                     GMLTIFLPDNRHKD"
     gene            52795..54447
                     /gene="ushA"
                     /locus_tag="NBI74_000403"
     CDS             52795..54447
                     /gene="ushA"
                     /locus_tag="NBI74_000403"
                     /EC_number="3.1.3.5"
                     /EC_number="3.6.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HCD1930780.1"
                     /translation="MKLLQRGVALALLTTFTLASETALAYEQDKTYKITVLHTNDHHG
                     HFWRNEYGEYGLAAQKTLVDGIRKEVAAEGGSVLLLSGGDINTGVPESDLQDAEPDFR
                     GMNLVGYDAMAIGNHEFDNPLTVLRQQEKWAKFPLLSANIYQKSTGERLFKPWALFKR
                     QDLKIAVIGLTTDDTAKIGNPEYFTDIEFRKPADEAKLVIQELQQTEKPDIIIAATHM
                     GHYDNGEHGSNAPGDVEMARALPAGSLAMIVGGHSQDPVCMAAENKKQVDYVPGTPCK
                     PDQQNGIWIVQAHEWGKYVGRADFEFRNGEMKMVNYQLIPVNLKKKVTWEDGKSERVL
                     YTPEIAENQQMISLLSPFQNKGKAQLEVKIGETNGRLEGDRDKVRFVQTNMGRLILAA
                     QMDRTGADFAVMSGGGIRDSIEAGDISYKNVLKVQPFGNVVVYADMTGKEVIDYLTAV
                     AQMKPDSGAYPQFANVSFVAKDGKLNDLKIKGEPVDPAKTYRMATLNFNATGGDGYPR
                     LDNKPGYVNTGFIDAEVLKAYIQKSSPLDVSVYEPKGEVSWQ"
     gene            complement(54485..54988)
                     /locus_tag="NBI74_000404"
     CDS             complement(54485..54988)
                     /locus_tag="NBI74_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001519543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1930781.1"
                     /translation="MKFDHLVDVSFHNFVFNGKFDFVELGQTMAWLEQNFVEPDSKND
                     MGNGFYIWLFGNVEFHFENNKLYMIWCDYLSQIHLGKAIRFDKGILNNVAELNVTRVF
                     NMLVNEGADLQIRRQSPHTLLIHVNRSGVTLWFEADEEQPGDPQNYMLMAFGLTHRDY
                     DARFQYS"
     gene            complement(54985..55785)
                     /locus_tag="NBI74_000405"
     CDS             complement(54985..55785)
                     /locus_tag="NBI74_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000439795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1930782.1"
                     /translation="MERITETLSIACADEIPDIALLRQTSREKVLHLHFASEAERYRE
                     EFGLVLKAQLDEFVVGMHSADPVIRIARLISLEEINRHQDFFEHCAKEYRKLATELIF
                     ALAEQLNVEMAENNPLVTFAPFKCNRKRKGKMGKWQYCFHGFHCTFENKKTEQNIEVP
                     LAYGFDFGDLDPYFFSGFIKSTPAWQPLPVAIYDDYHDGSRIIQQLLALGKLQKIPSP
                     IPQYTGVAAVDRSNVDIANFRSTLESRLHRCKLRWLLKHVKNSQRSER"
     gene            complement(55809..56288)
                     /gene="ybaK"
                     /locus_tag="NBI74_000406"
     CDS             complement(55809..56288)
                     /gene="ybaK"
                     /locus_tag="NBI74_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK"
                     /protein_id="HCD1930783.1"
                     /translation="MTPAVKLLEKNKISFQIHTYEHDPAETNFGDEVVKKLGLNPDQV
                     YKTLLVAVNGDMKHLAVAVTPVAGQLDLKKVAKALGAKKVEMADPMVAQRSTGYLVGG
                     ISPLGQKKRLPTIIDAPAQEFATIYVSGGKRGLDIELAASDLAKILDAKFADIARRD"
     gene            complement(56492..57286)
                     /locus_tag="NBI74_000407"
     CDS             complement(56492..57286)
                     /locus_tag="NBI74_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HCD1930784.1"
                     /translation="MDLLYRVKTLWAALRGNHYTWPAIDISLPGNRHFHLIGSIHMGS
                     HDMAPLPTRLLKKLKNADALIVEADVSTSDTPFANLPACEALEERISEDQLQNLQHIS
                     QEMGISPSLFSTQPLWQIAMVLQATQAQKLGLRAEYGIDYQLLQAAKQQHKPVIELEG
                     AENQIAMLLQLPDKGLALLDDTLTHWHTNARLLQQMMSWWLNAPPQNNDITLPNTFSQ
                     SLYDVLMHQRNLAWRDKLRAMPPGRYVVAVGALHLYGEGNLPQMLR"
     gene            57419..57721
                     /locus_tag="NBI74_000408"
     CDS             57419..57721
                     /locus_tag="NBI74_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000057540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HCD1930785.1"
                     /translation="MAQKKNIRSFRDAWLADFFVHSTPHRKIPAEIHTTLSRKLDIIN
                     AATSHRDLRSPPGNRYEELSGKLQEYSSIRVNKQYRLIFKWVNGKAEELFLDPHNY"
     gene            57757..58098
                     /locus_tag="NBI74_000409"
     CDS             57757..58098
                     /locus_tag="NBI74_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antidote protein"
                     /protein_id="HCD1930786.1"
                     /translation="MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNSVSAL
                     INNNRKLTTEMAFRLAKVFDTTVDFWLNLQAAVDLWEVENNMRTQEELGRIETVAEYL
                     ARREERAKKVA"
     gene            complement(58156..60660)
                     /gene="copA"
                     /locus_tag="NBI74_000410"
     CDS             complement(58156..60660)
                     /gene="copA"
                     /locus_tag="NBI74_000410"
                     /EC_number="7.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-exporting P-type ATPase CopA"
                     /protein_id="HCD1930787.1"
                     /translation="MSQTIDLTLDGLSCGHCVKRVKESLEQRPDVEQADVSITEAHVT
                     GTASAEQLIETIKQAGYDASVSHPKAKPLAESSIPSEALTAVSEALPAATADDDDSQQ
                     LLLSGMSCASCVTRVQNALQSVPGVTQARVNLAERTALVMGSASPQDLVQAVEKAGYG
                     AEAIEDDAKRRERQQETAVATMKRFRWQAIVALAVGIPVMVWGMIGDNMMVTADNRSL
                     WLVIGLITLAVMVFAGGHFYRSAWKSLLNGAATMDTLVALGTGVAWLYSMSVNLWPQW
                     FPMEARHLYYEASAMIIGLINLGHMLEARARQRSSKALEKLLDLTPPTARLVTDEGEK
                     SVPLAEVQPGMLLRLTTGDRVPVDGEITQGEAWLDEAMLTGEPIPQQKGEGDSVHAGT
                     VVQDGSVLFRASAVGSHTTLSRIIRMVRQAQSSKPEIGQLADKISAVFVPVVVVIALI
                     SAAIWYFFGPAPQIVYTLVIATTVLIIACPCALGLATPMSIISGVGRAAEFGVLVRDA
                     DALQRASTLDTVVFDKTGTLTEGKPQVVAVKTFADIDEAQALRLAAALEQGSSHPLAR
                     AILDKAGDMQLPQVNGFRTLRGLGVSGEAEGHALLLGNQALLNDQQVDTKAIEADISA
                     QASQGATPVLLAVDGKAVALLAVRDPLRSDSVAALQRLHKAGYRLVMLTGDNPTTANA
                     IAKEAGIDEVIAGVLPDGKAEAIKRLQSEGRQVAMVGDGINDAPALAQADVGIAMGGG
                     SDVAIETAAITLMRHSLMGVADALAISRATLRNMKQNLLGAFIYNSIGIPVAAGILWP
                     FTGTLLNPVVAGAAMALSSITVVSNANRLLRFKPKE"
     gene            60922..61854
                     /gene="glsA"
                     /locus_tag="NBI74_000411"
     CDS             60922..61854
                     /gene="glsA"
                     /locus_tag="NBI74_000411"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HCD1930788.1"
                     /translation="MLDANKLQQAVDQAYTQFHSLNGGQNADYIPFLANVPGQLAAVA
                     IVTSDGNVYSAGDSDYRFALESISKVCTLALALEDVGPQAVQDKVGADPTGLPFNSVI
                     ALELHGGKPLSPLVNAGAIATTSLINAENTEQRWQRILHIQQQLAGEQVALSDEVNQS
                     EQTTNFHNRAIAWLLYSAGYLYCDAMEACDVYTRQCSTLINTIELATLGATLAAGGVN
                     PLTHKRVLQADNVPYILAEMMMEGLYGRSGDWAYRVGLPGKSGVGGGILAVVPGVMGI
                     AAFSPPLDEEGNSVRGQKMVASVAKQLGYNVFKG"
     gene            61857..63149
                     /locus_tag="NBI74_000412"
     CDS             61857..63149
                     /locus_tag="NBI74_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCD1930789.1"
                     /translation="MMNTEGNNGNKPLGLWNVVSIGIGAMVGAGIFALLGQAALLMEA
                     STWVAFAFGGIVAMFSGYAYARLGASYPSNGGIIDFFRRGLGNGVFSLALSLLYLLTL
                     AVSIAMVARAFGAYAVQFLHEGSQEEHLILLYALGIIAVMTLFNSLSNHAVGRLEVIL
                     VGIKMMILLLLIIAGVWSLQPAHISVSAPPSSGAFFSCIGITFLAYAGFGMMANAADK
                     VKDPEVIMPRAFLVAIGVTTLLYISLALVLLSDVSALELEKYADTAVAQAASPLLGHV
                     GYVIVVIGALLATASAINANLFAVFNIMDNMGSERELPKLMNKPLWRQSTWGNIIVVV
                     LIMLMTAALNLGSLASVASATFLICYLAVFVVAIRLRHDIHASLPILIVGTLVMLLVI
                     VGFIYSLWSQGSRALIWIIGALLLSLIVAMVMKRNKTV"
     gene            63274..63681
                     /gene="cueR"
                     /locus_tag="NBI74_000413"
     CDS             63274..63681
                     /gene="cueR"
                     /locus_tag="NBI74_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(I)-responsive transcriptional regulator"
                     /protein_id="HCD1930790.1"
                     /translation="MNISDVAKITGLTSKAIRFYEEKGLVTPPMRSENGYRTYTQQHL
                     NELTLLRQARQVGFNLEESGELVNLFNDPQRHSADVKRRTLEKVAEIEQHIEELQSMR
                     NQLLALANACPGDDSADCPIIENLSGCCHHRAG"
     gene            complement(63682..64417)
                     /locus_tag="NBI74_000414"
                     /pseudo
     CDS             complement(63682..64417)
                     /locus_tag="NBI74_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001601795.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(64511..64969)
                     /locus_tag="NBI74_000415"
     CDS             complement(64511..64969)
                     /locus_tag="NBI74_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HCD1930791.1"
                     /translation="MMELMVVHPHIFWLSLGGLLLAAEMLGGNGYLLWSGVAAVITGL
                     VVWLLPLGWEWQGVMFAVLTLLAAWLWWKWLSRRVREQKHSDSHLNQRGQQLIGRRFV
                     LESPLVNGRGHMRVGDSSWPVSASEDLGAGTHVEVIAIEGITLIIRAVIA"
     gene            complement(64966..65883)
                     /locus_tag="NBI74_000416"
     CDS             complement(64966..65883)
                     /locus_tag="NBI74_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH/Band 7/PHB domain protein"
                     /protein_id="HCD1930792.1"
                     /translation="MLIFIPILIFVALVIVGAGVKIVPQGYQWTVERFGRYTKTLQPG
                     LSLVVPFMDRIGRKINMMEQVLDIPSQEVISKDNANVTIDAVCFIQVIDAPRAAYEVS
                     NLELAIINLTMTNIRTVLGSMELDEMLSQRDSINSRLLHIVDEATNPWGIKVTRIEIR
                     DVRPPAELISSMNAQMKAERTKRAYILEAEGIRQAEILKAEGEKQSQILKAEGERQSA
                     FLQAEARERSAEAEARATKMVSEAIASGDIQAVNYFVAQKYTEALQQIGSSSNSKVVM
                     MPLEASSLMGSIAGIAELVKDSANKRTQP"
     gene            66029..66706
                     /gene="fetA"
                     /locus_tag="NBI74_000417"
     CDS             66029..66706
                     /gene="fetA"
                     /locus_tag="NBI74_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter ATP-binding protein FetA"
                     /protein_id="HCD1930793.1"
                     /translation="MQENSPLLQLQNVGYLAGDTKILNNINFSLRAGEFKLITGPSGC
                     GKSTLLKIVASLISPTSGTLLFEGEDVSTLKPEIYRQQVSYCAQTPTLFGDTVYDNLI
                     FPWQIRNQQPDPAIFLDFLERFALPDSILTKNIAELSGGEKQRISLIRNLQFMPKVLL
                     LDEITSALDESNKHNVNEMIHRYVREQNIAVLWVTHDKDEINHADKVITLQPHAGEMQ
                     EARYELA"
     gene            66693..67472
                     /gene="fetB"
                     /locus_tag="NBI74_000418"
     CDS             66693..67472
                     /gene="fetB"
                     /locus_tag="NBI74_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron export ABC transporter permease subunit
                     FetB"
                     /protein_id="HCD1930794.1"
                     /translation="MNSHNITNESLALALMLVVVAILISHKEKLALEKDILWSVGRAI
                     IQLIIVGYVLKYIFSVDDASLTLLMVLFICFNAAWNAQKRSKYIAKAFISSFIAITVG
                     AGITLAVLILSGSIEFIPMQVIPIAGMIAGNAMVAVGLCYNNLGQRVISEQQQIQEKL
                     SLGATPKQASAILIRDSIRAALIPTVDSAKTVGLVSLPGMMSGLIFAGIDPVKAIKYQ
                     IMVTFMLLSTASLSTIIACYLTYRKFYNSRHQLVVTQLKKK"
     gene            complement(67655..68509)
                     /gene="cnoX"
                     /locus_tag="NBI74_000419"
     CDS             complement(67655..68509)
                     /gene="cnoX"
                     /locus_tag="NBI74_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperedoxin"
                     /protein_id="HCD1930795.1"
                     /translation="MSVENIVNINESNLQQVLEQSMTTPVLFYFWSERSQHCLQLTPI
                     LESLAAQYNGQFILAKLDCDAEQMIAAQFGLRAIPTVYLFQNGQPVDGFQGPQPEEAI
                     RALLDKVLPREEELKAQQAMQLMQEGNYTDALPLLKDAWQLSNQNGEIGLLLAETLIA
                     LNRSEDAEAVLKTIPLQDQDTRYQGLVAQIELLKQAADTPEIQQLQQQVAENPEDAAL
                     ATQLALQLHQVGRNEEALELLFGHLRKDLTAADGQTRKTFQEILAALGTGDALASKYR
                     RQLYALLY"
     gene            complement(68570..69379)
                     /gene="ybbO"
                     /locus_tag="NBI74_000420"
     CDS             complement(68570..69379)
                     /gene="ybbO"
                     /locus_tag="NBI74_000420"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HCD1930796.1"
                     /translation="MTHKATEILTGKVMQKSVLITGCSSGIGLESALELKRQGFHVLA
                     GCRKPDDVERMNSMGFTGVLIDLDSPESVDRAADEVIALTDNCLYGIFNNAGFGMYGP
                     LSTISRAQMEQQFSANFFGAHQLTMRLLPAMLPHREGRIVMTSSVMGLISTPGRGAYA
                     ASKYALEAWSDALRMELRHSGIKVSLIEPGPIRTRFTDNVNQTQSDKPVENPGIAARF
                     TLGPEAVVGKVRHAFISEKPKMRYPVTLVTRAVMVLKRLLPGRVMDKILQG"
     gene            complement(69369..69992)
                     /gene="tesA"
                     /locus_tag="NBI74_000421"
     CDS             complement(69369..69992)
                     /gene="tesA"
                     /locus_tag="NBI74_000421"
                     /EC_number="3.1.1.5"
                     /EC_number="3.1.2.14"
                     /EC_number="3.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional acyl-CoA thioesterase I/protease
                     I/lysophospholipase L1"
                     /protein_id="HCD1930797.1"
                     /translation="MNFNNVFRWHLPFLFLVLLTFRAAAADTLLILGDSLSAGYRMSA
                     SAAWPALLNDKWQSKTSVVNASISGDTSQQGLARLPALLKQHQPRWVLVELGGNDGLR
                     GFQPQQTEQTLRQILQDVKAANAEPLLMQIRLPANYGRRYNEAFSAIYPKLAKEFDVP
                     LLPFFMEEVYLKPQWMQDDGIHPNRDAQPFIADWMAKQLQPLVNHDS"
     gene            69963..70649
                     /locus_tag="NBI74_000422"
     CDS             69963..70649
                     /locus_tag="NBI74_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCD1930798.1"
                     /translation="MPAENIVEVHHLKKSVGQGEHELSILTGVELVVKRGETIALVGE
                     SGSGKSTLLAILAGLDDGSSGEVSLVGQPLHNMDEEARAKLRAKHVGFVFQSFMLIPT
                     LNALENVELPALLRGESSAESRNGAKALLEQLGLGKRLDHLPAQLSGGEQQRVALARA
                     FNGRPDVLFADEPTGNLDRQTGDKIADLLFSLNREHGTTLIMVTHDLQLAARCDRCLR
                     LVNGQLQEEA"
     gene            70646..73060
                     /locus_tag="NBI74_000423"
     CDS             70646..73060
                     /locus_tag="NBI74_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCD1930799.1"
                     /translation="MIARWFWREWRSPSLLIVWLALSLAVACVLALGNISDRMEKGLS
                     QQSREFMAGDRALRSSREVPQAWLEEAQKRGLKVGKQLTFATMTFAGDTPQLANVKAV
                     DDIYPMYGDLQTNPPGLKPQAGSVLLAPRLMALLNLKTGDTIDVGDATLRIAGEVIQE
                     PDSGFNPFQIAPRLMMNLADVDKTGAVQPGSRVTWRYKFGGSENQLDGYEKWLLPQLK
                     PEQRWYGLEQDEGALGRSIERSQQFLLLSALLTLLLAVAAVAVAMNHYCRSRYDLVAI
                     LKTLGAGRAQLRKLIVGQWLMVLVLSAVTGGAIGLLFENVLMVLLKPVLPAALPPASL
                     WPWLWALGTMTVISLLVGLRPYRLLLATQPLRVLRNDVVANVWPLKFYLPIVTVVVVL
                     LLAGLMGGSMLLWAVLAGAVVLALLCGVLGWMLLNVLRRMTLKSLPLRLAVSRLLRQP
                     WATLSQLSAFSLSFMLLALLLVLRGDLLDRWQQQLPPESPNYFLINIATEQVTPLKAF
                     LAEHQIVPESFYPVVRARLTAINDKPTEGNGDEALNRELNLTWQNTRPDHNPIVAGNW
                     PPKAGEVSMEEGLAKRLNVALGDTVTFMGDTQEFRAKVTSLRKVDWESLRPNFYFIFP
                     EGALDGQPQSWLTSFRWENGNGMLTQLNRQFPTISLLDIGAILKQVGQVLEQVSRALE
                     VMVVLVTACGMLLLLAQVQVGMRQRHQELVVWRTLGAGKKLLRTTLWCEFAMLGFVSG
                     LVAAIGAETALAVLQSKVFDFPWEPDWRLWIVLPCSGALLLSLCGGWLGARLLKGKAL
                     FRQFVG"
BASE COUNT        17947 a        18453 c        19469 g        17271 t
ORIGIN      
        1 taacaaataa ctatcattag ggtgacaact gggtggtgag aaaataagga ctcatcagct
       61 gaatcgtgta aaaaaaatag aattcataaa tggcgtcaat aatttccaga cgctaagccg
      121 cacaaaagaa caaatatcaa taagcgatgc tgatgtttat ttagccgtaa taattacggc
      181 gagtgattac tacagctaaa taatatttac agattacgtc agatgtgttt ttcgtagcgc
      241 cagataatcc agcaaaccac caagcacgac gccagagagg gcgactaaaa ccccgacttc
      301 cagcagcgca ggcaggaaag agaagtgcgt caaatccagc gcgtccatcg tcaacagcag
      361 taaccacacg gccaacaggc tgatgcccgc cgccagtgtc cacatggcaa aggcataacc
      421 tgcaggatat tcggtacggg agataaagtt gtcatttact cgtgtatatt caagcgtctg
      481 gcggcaaacc agcagcaaaa taagccctgg tgcggcagcg gcgacggaga atagatagaa
      541 tgtcgaccag ccgtgtgctt caacaaacca acccgccacg gggccgacat aaactcgccc
      601 tacagcagaa agcgctgaga gcagggcaaa ttgagtagcg gaaaatgact tattacacag
      661 cgtcattaac agcgcgacaa aggctgatgt acccatcccg ccacagaggt tttcgaaaaa
      721 gacggctgca cccatgctgt agagatgctt atcagtaatc gacagcagcc agtaaccggc
      781 gttagatgca ccttgtaaaa tgccgaaaat cagcagtgca cggaacagcg acaggcgctg
      841 catcaaaatc ccaccgtaca atgcgccaac aatggtcgcc agtaagccaa gcgttttgtt
      901 aaccacgcct acttcacccg catcaaaccc gacgccgcga atcaaaaacg tggttgtcag
      961 gctcatagcg aatgcgtcgc ccagcttata cagcacgata agaagcaaaa taagccaggc
     1021 attattgcga ccaaagaaat ctcgcagagg tgcaacaacc gcttgttcca gcgttttggg
     1081 cacaggaatg gtgtcggtgg gttctggtgc aagcaacgtc gcgataatac aggggatcaa
     1141 cagcgccgcc atcaaccagt acatgccctg ccagcccagc catttatctg ccagccacag
     1201 ggctaagccg ccggaaacca gcatccctaa acggtaaccc agcacgctga ttgccgcgcc
     1261 cgcaccacgt tcttctgccg ggagcacatc ggttttccac gcatcgaaga caatatcctg
     1321 ggaggcagag caaaaagcga tcaccaccgc cagtgccgcc atccagcgga gctgggtgcc
     1381 tggttcgaga aaacccatcg ccgcaatggc gactaataac agaatttgcg tggcgagcag
     1441 ccaaccgcgc cgccgcccga aaaatggggg cgtgtagcgg tccatcagtg gtgaccagag
     1501 gaatttaaaa acgtaagcct ggcctaccag agagaagaaa ccaatggttt tgagatcgat
     1561 attctcgacc gtcatccagg cctgtaaggt gccggacgtc agggcgagcg gtagcccgga
     1621 agcaaaaccc aggatcagca atatggctga acgcggctgt tgaaaaatac gtaaatattg
     1681 actggacatg ggcgtctaca ggcccggctt gcaccgggcc agagggcaga aattaacgcg
     1741 cgttctgctt gatgaattcg tggatgctgg tgtcctgaga catatcagcg atggtatcgg
     1801 tcagcacact gttaaccgca tcggcgatat ttttgttgga ggcctggaac gcaccttcaa
     1861 cgttgtagct ggcacgatag tttttggtca ttttgttgcc attctgcgcg gtagcgatga
     1921 tggcgatatc cgctttggtc gcgatgttgt agcgcacgtt gccctgggac acgtcagcat
     1981 acagttggct aacgatgatt tgcagattaa ccgggccatt cggaccaacc atgtatccac
     2041 gggcggtcat ttgtttttcc agcacttctt gcagcaggaa acgcagatcg cgggaggcgg
     2101 tcagggtaac gatttgatta tcgcgggtga cttttgccag cgcctgatcg gtacgctgat
     2161 cggcaccatt aatgcttacg gtgacgccca tcaggcttgg atcctgctgt ggcagtgtaa
     2221 tcgtcgggga aacttcgata gttgttggcg gttttgcgca tcctgcaagc ataaacagag
     2281 caactaacgg gaagaggatt tttttgaaca tgttcgggct ctcagagact cttaagcgtg
     2341 tttggtaaaa attcccgcca tcataacatt gccaacggcg aggggaagtg ggtaaggcat
     2401 gtaaattcat catgttgacg aaataatcac ccctggtaaa agaaacgctg atgcgaggcc
     2461 tgtgtttcaa tctttaaatc agtaaacttc atacgcttga cggaaaaacc aggacgaaac
     2521 ctaaatattt gttgttaagc tgcaatggaa acggtaaaag cggctagtat ttaaagggat
     2581 ggatgacatc tcagcgttgt cggaggagat atttcatgat gatacgtgag cggatagaag
     2641 aaaaattaag ggcggcgttc caacccgtat tcctcgaagt agtggatgaa agctatcgtc
     2701 acaatgtccc agccggctct gaaagccatt ttaaagttgt gctggtcagc gatcgtttta
     2761 cgggtgaacg ttttctgaat cgtcatcgaa tgatttacag tactttagcg gaggaactct
     2821 ctactaccgt tcatgcgctg gctctgcata cttacactat taaggagtgg gaagggttgc
     2881 aggacaccgt ctttgcctct cctccctgtc gtggagcagg aagcatcgcg taaaaacgca
     2941 tttgcaactg tcggcgcttt tccagtatgt tgctaaagat tttatgaaaa acggcctgcg
     3001 ggccgttttg ttttgtctgg attttgcgct ttttgcccag cattcagacg aaaatcgccc
     3061 gggaattgtg aaaaaatacg cgacagcgcg caataaccgt tctcgactca taaaagtgat
     3121 gccgctataa tgccgcgtcc tatttgaatg ctttcgggat gattctggta acagggaatg
     3181 tgattgatta taagaacatc ccggttccgc gaagccaaca acctgtgctt gcggggtaag
     3241 agttgaccga gcactgtgat tttttgaggt aacaagatgc aagtttcagt tgaaaccact
     3301 caaggccttg gccgccgtgt aacgattact atcgctgctg acagcatcga gaccgctgtt
     3361 aaaagcgagc tggtcaacgt tgcgaaaaaa gtacgtattg acggcttccg caaaggcaaa
     3421 gtgccaatga atatcgttgc tcagcgttat ggcgcgtctg tacgccagga cgtcctgggc
     3481 gacctgatga gccgtaactt cattgacgcc atcattaaag aaaaaatcaa tccggctggc
     3541 gcaccgacct atgttccggg cgaatacaag ctgggtgaag acttcaccta ctctgtagag
     3601 tttgaagttt atccggaagt tgaactgcag ggtctggaag cgatcgaagt tgaaaaaccg
     3661 attgttgaag tgaccgacgc tgacgttgac ggcatgctgg atactctgcg taaacagcag
     3721 gcaacctgga aagaaaaaga cggcgctgtt gaagcagaag accgtgtgac catcgacttc
     3781 accggttctg tagacggaga agagttcgaa ggcggtaaag cgtctgattt cgtactggcg
     3841 atgggtcagg gtcgcatgat cccgggcttt gaagacggta tcaaaggcca caaagctggc
     3901 gaagagttca ccatcgacgt gaccttcccg gaagagtacc acgcagaaaa cctgaaaggt
     3961 aaagcagcga aattcgctat caacctgaag aaagttgaag agcgtgaact gccggaactg
     4021 actgaagaat tcatcaaacg tttcggcgtt gaagatggtt ccgtagaagg tctgcgcgct
     4081 gaagtgcgta aaaacatgga gcgcgagctg aagagcgcca tccgtaaccg cgttaagtct
     4141 caggcgatcg aaggtctggt aaaagctaac gacatcgatg taccggctgc gctgatcgac
     4201 agcgaaatcg acgttctgcg tcgccaggct gcacagcgtt tcggtggcaa cgaaaaacaa
     4261 gctctggaac tgccgcgcga actgttcgaa gaacaggcta aacgccgcgt agttgttggc
     4321 ctgctgctgg gcgaagttat ccgcaccaac gagctgaaag ctgacgaaga gcgcgtgaaa
     4381 ggcttgatcg aagagatggc ttctgcgtac gaagatccga aagaagttat cgagttctac
     4441 agcaaaaaca aagaactgat ggacaacatg cgcaatgttg ctctggaaga acaggctgtt
     4501 gaagctgtac tggcgaaagc gaaagtgact gaaaaagaaa ccactttcaa cgagctgatg
     4561 aaccagcagg cgtaatttac gtagcataac gcgctaaatt cgcacaaagg cccgtcaccg
     4621 ccaggtggtg ggcttttttt tgtcatgaat tttgcatgga accgtgcgaa aagcctcttt
     4681 cggtgttagc gtaacaacaa aagattgtta tgcttgaaat atggtgatgc cgtacccata
     4741 acacagggac tagctgataa tccgtccata aggttacaat cggtacagca ggttttttca
     4801 attttatcca ggagacggaa atgtcataca gcggcgaacg agataacttt gcaccccata
     4861 tggcgctggt gccgatggtc attgaacaga cctcacgagg tgagcgctct tttgatatct
     4921 attctcgtct acttaaggaa cgcgtcattt ttctgactgg ccaggttgaa gaccacatgg
     4981 ctaacctgat tgtggcgcag atgctgttcc tggaagcaga aaacccagaa aaagatatct
     5041 atctgtacat taactctcca ggcggggtga ttactgccgg gatgtctatc tatgacacca
     5101 tgcagtttat caagcctgat gtcagcacca tctgtatggg ccaggcggcc tcgatgggcg
     5161 ctttcttgct gactgcaggg gcaaaaggta aacgtttctg cctgccgaat tcgcgcgtga
     5221 tgattcacca gccgttgggc ggctaccagg gccaggcgac cgatatcgaa attcatgccc
     5281 gtgaaattct gaaagttaaa gggcgcatga atgaacttat ggcgcttcat acgggtcaat
     5341 cattagaaca gattgaacgt gataccgagc gcgatcgctt cctttctgcc cctgaagcgg
     5401 tggaatacgg tctggtcgat tcgattctga cccatcgtaa ttgatgccag aggcgcaact
     5461 gtgccgctat acttatccag ggcggcacaa cgctgtaagc ggcttgcgcc tgagaatggc
     5521 atttgcgtcg tcgtgtgcgg cacaaagaac aaagaagagg ttttgaccca tgacagataa
     5581 acgcaaagat ggctcaggca aattgctgta ttgctctttt tgcggcaaaa gccagcatga
     5641 agtgcgcaag ctgattgccg gtccatccgt gtatatctgc gacgaatgtg ttgatttatg
     5701 taacgacatc attcgcgaag agattaaaga agttgcaccg catcgtgaac gcagtgcgct
     5761 accgacgccg catgaaattc gcaaccacct ggacgattac gttatcggtc aggaacaggc
     5821 gaaaaaagtg ctggcggtcg cggtatacaa ccactacaaa cgtctgcgca acggagatac
     5881 cagcaatggc gtcgagttgg gcaaaagtaa cattctgctg atcggtccga ccggttccgg
     5941 taaaacgctg ctggccgaaa cgctggcgcg cctgctggac gttccgttca ccatggctga
     6001 cgcaaccacg ctgaccgaag ccggttatgt gggcgaagac gttgaaaaca tcattcagaa
     6061 gctgttgcag aagtgcgatt acgacgtaca gaaagcgcag cgcgggattg tctacatcga
     6121 tgaaatcgac aagatttctc gtaagtcaga caacccgtcc attacccgag acgtttccgg
     6181 tgaaggcgta cagcaggcac tgttaaaact gatcgaaggt acggtagctg ctgttccgcc
     6241 gcaaggtggg cgtaaacatc cgcagcagga attcttgcag gttgatacct ctaagatcct
     6301 gttcatctgt ggcggcgcat ttgctggcct ggataaagtg attgccaatc gtgttgaaac
     6361 cggctccggc attggttttg gcgcgacggt gaaagccaag tccgacaaag ccagcgaagg
     6421 tgaactgctg gcgcaggttg aaccggaaga tctgatcaag tttggtctga tccctgagtt
     6481 cattggtcgt ctgccggttg tcgcaacgtt gaatgaactg agcgaagaag ctctgattca
     6541 gatcctcaaa gagccgaaaa acgccctgac caagcagtat caggcgctgt ttaatctgga
     6601 aggcgtggat ctggaattcc gtgacgaggc gctggatgct atcgctaaga aagcgatggc
     6661 gcgtaaaacc ggtgcccgtg gcctgcgttc catcgtagaa gccgcactgc tcgataccat
     6721 gtacgatctg ccgtccatgg aagatgtcga aaaagtggtt atcgacgagt cggtaattga
     6781 tggtcaaagc aaaccgttgc tgatttatgg taagccggaa gcgcaacagg catctggtga
     6841 ataattaacc attcccatac aattagttaa ccgaaaaggg gggattttat ctccccttta
     6901 atttttcctc tattctcggc gttgaatgtg ggggaaacat ccccatatac tgacgtacat
     6961 gttaatagat ggcgtgaagc acagtcgtgt catctgatta cctggcggaa attaaactaa
     7021 gagagagctc tatgaatcct gagcgttctg aacgcattga aatccccgta ttgccgctgc
     7081 gcgatgtggt ggtttatccg cacatggtca tccccttatt tgtcgggcgg gaaaaatcca
     7141 tccgttgtct ggaagcggcg atggaccatg ataaaaaaat tatgctggtc gcgcagaaag
     7201 aagcttcaac ggatgagccg ggtgtaaacg atcttttcac cgtcgggacc gtggcctcta
     7261 tattgcagat gctgaaactg cctgacggca ccgtcaaagt gctggtcgag gggttacagc
     7321 gcgcgcgtat ttctgcgctc tctgacaatg gcgaacactt ttctgcgaag gcggagtatc
     7381 tggagtcgcc gaccattgat gagcgggaac aggaagtgct ggtgcgtact gcaatcagcc
     7441 agttcgaagg ctacatcaag ctaaacaaaa aaatcccacc agaagtgctg acgtcgctga
     7501 atagcatcga cgatccggcg cgtctggcgg ataccattgc tgcacatatg ccgctgaaac
     7561 tggctgacaa acagtccgtt ctggagatgt ccgacgttaa cgaacgtctg gaatatctga
     7621 tggcaatgat ggaatcggaa atcgatctgc tgcaggttga gaaacgcatt cgcaaccgcg
     7681 ttaaaaagca gatggagaaa tcccagcgtg agtactatct gaacgagcaa atgaaagcta
     7741 ttcagaaaga actcggtgaa atggacgacg cgccggacga aaacgaagcc ctgaagcgca
     7801 aaatcgacgc ggcgaagatg ccgaaagagg caaaagagaa agcggaagca gagctgcaga
     7861 agctgaaaat gatgtctccg atgtcggcag aagcgaccgt agtgcgtgga tatatcgact
     7921 ggatggtaca ggtgccgtgg aatgcgcgta gcaaggtcaa aaaagacctg cgtcaggcgc
     7981 aggaaatcct tgataccgac cattatggtc tggagcgcgt gaaagatcgt atccttgagt
     8041 atcttgcggt tcaaagccgt gtcaacaaaa tcaagggtcc gatcctctgc ctggtagggc
     8101 cgccgggggt aggtaaaacg tccctgggtc agtccatcgc caaagccacc gggcgtaaat
     8161 atgtccgtat ggcgctgggc ggcgtgcgtg atgaagcgga aatccgtggt caccgccgta
     8221 cttacatcgg ttctatgccg ggtaaactga tccagaaaat ggcgaaagtg ggcgtgaaaa
     8281 acccgctgtt cctgctcgat gagatcgaca aaatgtcttc tgacatgcgt ggtgatccgg
     8341 cctccgcact gcttgaagtg ctggatccag agcagaacgt agcgttcagc gaccactacc
     8401 tggaagtgga ttacgatctc agcgacgtaa tgtttgtcgc tacgtcgaac tccatgaaca
     8461 ttccggcacc gttgctggat cgtatggaag tgattcgcct ctccggttat accgaagatg
     8521 aaaaactgaa catcgccaaa cgtcacctgc tgccgaagca gattgaacgt aatgcattga
     8581 aaaaaggtga gctgaccgtc gacgatagcg ccattatcgg cattattcgt tactacaccc
     8641 gtgaggcggg cgtgcgtggt ctggagcgtg aaatctccaa actgtgtcgc aaagcggtta
     8701 agcagttact gctcgataag tcattaaaac atatcgaaat taacggtgat aacctgcatg
     8761 actacctcgg tgttcagcgt ttcgactatg ggcgcgcgga taacgaaaac cgtgtcggcc
     8821 aggtaaccgg tctggcgtgg acggaagtgg gcggtgactt gctgaccatt gaaaccgcgt
     8881 gcgttccggg gaaaggcaaa ctgacctata ccggttcgct cggcgaagtg atgcaggagt
     8941 ctattcaggc ggcgttaacg gtggttcgtg cgcgtgcgga aaaactgggg atcaaccctg
     9001 atttttacga aaaacgtgac atccacgtcc acgtaccgga aggtgcgacg ccgaaagatg
     9061 gtccgagtgc cggtattgcc atgtgcaccg cgctggtttc ttgcctgacc ggtaacccgg
     9121 ttcgtgccga tgtggcaatg accggtgaga tcactctgcg tggtcaggta ctgccgatcg
     9181 gtggtttgaa agaaaaacta ctggcagcgc atcgcggcgg gattaaaaca gtgttaattc
     9241 cgttcgaaaa taaacgcgat ctggaagaga ttcctgacaa cgtaattgcc gatctggaca
     9301 ttcatcctgt gaagcgcatt gaggaagttc tgactctggc gctacaaaat gaaccgtctg
     9361 gcatgcaggt tgtgactgca aaatagtgac ctcgcgcaaa atgcactaat aaaaacaggg
     9421 ctggcaggct aattcgggct tgccagcctt tttttgtctc gctaagttag atggcggatc
     9481 gggcttgccc ttattaaggg gtgttgtaag gggatggctg gcctgatata actgctgcgc
     9541 gttcgtacct tgaaggattc aagtgcgata taaattataa agaggaagag aagagtgaat
     9601 aaatctcaat tgatcgacaa gattgctgca ggggctgata tctctaaagc tgcggctggc
     9661 cgtgcgttag atgctattat tgcttccgta actgaatctc tgaaagaagg ggatgatgta
     9721 gcactggtag gttttggtac ttttgccgtt aaggaacgtg ctgcccgtac tggccgcaac
     9781 ccgcagaccg gtaaagagat caccatcgct gctgctaaag taccgagctt ccgtgcaggt
     9841 aaagcactga aagacgcggt aaactaagcg ttgtccccag tggggatgtg acgaagttca
     9901 agggcgcatc tactgatgtg ccttttttat ttgtattcgg tgactttctg cgtcttgtgg
     9961 gctgacaatt gcccccgttt cttgtcacaa taggcctttg cgcgcatcga tacgttgcgt
    10021 gaggtacaca gtcatctaca gcggagtgtt gttacaccat gatggacagc ttacgcacgg
    10081 ctgcaaacag tctcgtgctc aagattattt tcggtatcat tatcgtgtcg ttcatattga
    10141 ccggcgtgag tggttacctg attggcggag gcaataacta cgccgcaaaa gtgaatgacc
    10201 aggaaatcag ccgtgggcaa ttcgagaacg ccttcaacag cgagcgtaat cgcatgcagc
    10261 aacagctggg cgatcaatac tccgagctgg cagcgaacga aggctatatg aaaaccctgc
    10321 gtcaacaggt gctgaatcgt ctgatcgacg aggcgcttct ggatcagtac gctcgtgagc
    10381 tgaaactggg tatcagcgat gagcaggtta aacaggcgat tttcgcgacc ccagccttcc
    10441 aggttgacgg caaatttgat aacagccgct ataacggtat cctcaaccag atggggatga
    10501 ccgccgatca gtacgcccag gcgctgcgta accagctcac tacccaacag ctgattaacg
    10561 gcgttgccgg taccgatttt atgctgaaag gtgaaaccga cgagctggcg gcactggtag
    10621 ctcaacaacg cgtggtgcgt gaagcggcta tcgatgttaa cgcgctggcg gcgaagcagc
    10681 ctgtgaccga acaggaaatt gccagctact acgaacaaaa caaaaacaat ttcatgacgc
    10741 cggaacaatt ccgcgtgagt tacatcaagc tggatgccgc gacgatgcag caaccggtta
    10801 gcgatgcgga tatccagagc tactacgatc agcatcagga tcaattcacc cagccgcagc
    10861 gtacccgtta cagcatcatc cagaccaaaa ctgaagatga agcgaaagcg gtacttgatg
    10921 agctgaataa gggcggtgat tttgctgcat tagccaaaga aaaatctgcc gatattatct
    10981 ctgcccgtaa cggcggtgat atgggttggt tagaagatgc cactatcccg gacgaactga
    11041 aaaatgcggg tctgaaagaa aaaggccaac tgtctggtgt tatcaaatct tcggtcggtt
    11101 tcctgattgt acgtctggac gacattcagc cagcgaaagt gaaatcgtta gacgaagtac
    11161 gtgacgacat tgcggcgaaa gtgaaacacg aaaaagccct cgatgcgtac tacgcgctgc
    11221 agcagaaagt gagcgatgcg gcaagcaacg acaccgagtc tctggccggt gcagagcaag
    11281 ctgccggcgt taaagccact cagacgggtt ggttcagcaa agataacctg ccggaagagt
    11341 tgaacttcaa gccagttgcc gacgctatct tcaacggcgg tctggtaggt gaaaacggcg
    11401 cgccgggcat caactctgac atcatcaccg tagacggcga ccgcgcattc gtgctgcgca
    11461 tcagcgagca caaaccggaa gcggtgaaac cgttggcaga tgttcaggaa caagttaagg
    11521 cactggttca gcacaacaaa gctgaacaac aggcgaaagt ggatgctgag aaactgctgg
    11581 ttgatttgaa agccggcaaa ggtgcggaag cgatgcaggc tgccggtctg aaatttggcg
    11641 agccgaaaac cttaagccgt tccggtcgtg acccgattag ccaggctgcg tttgcactgc
    11701 cgctgccagc gaaagacaaa ccgagctacg gtatggcgac cgatatgcaa ggtaatgtgg
    11761 ttctgctggc gctggatgaa gtgaaacaag gttcaatgcc ggaagatcag aaaaaagcga
    11821 tggtgcaggg tatcacccag aacaacgcac aaatcgtctt tgaagctctg atgagtaacc
    11881 tgcgtaaaga ggcgaaaatc aaaattggcg atgcgctgga acagcaataa tcctgaagcc
    11941 gcctcgcaaa aaaatgcttt gcagctgtaa caagaaaagg tcgctttcgc ggccttttcc
    12001 atttctgaac attgccattt gtttactgtt ttcactgccg ttaaggtgat tccactgtta
    12061 acaaacaagg agaaaacagt atgaaacacg gaattaaagc actgctcatt accctgtccc
    12121 tggcctgtgc cggaatgtct catagtgctc tggcggcagc tcctgcggca aaaccgacaa
    12181 cggtagaaac caaagcggaa gctcctgcag cacaaagtaa agcagcagta ccggcgaaag
    12241 ccagtgacga agaaggcacc cgggtcagca ttaataatgc cagcgcggaa gagttagccc
    12301 gcgcgatgaa tggcgttggc ctgaagaaag cgcaggcgat tgtcagttat cgcgaagagt
    12361 acggtccgtt taaaacggtg gaggatctaa agcaggtgcc ggggatgggc aattcgctgg
    12421 tggaacgtaa tatggcggta ttaaccctgt aattaatttg catagtggca attttttgcc
    12481 agacttaaga ggtcatacca gttatgacct ctgtacttat aacagttctt ataacaacaa
    12541 cgtaaggtta ttgcgctatg caaacacaaa tcaaagttcg tggatatcat ctcgacgttt
    12601 accagcacgt aaacaacgcc cgctaccttg aatttctcga agaagcccgc tggcatgggt
    12661 tggaaaatag cgacagtttt cactggatga cggcccataa catcgctttc gtcgtggtca
    12721 atattaatat taactatcgc cgcccggcgg tattaagcga cctgttaacc attaccagtc
    12781 agttgcagca attaaacggt aaaagtggca tcttaagcca ggtcattaca ctggagccgg
    12841 aaggacaggt ggtagcggat gcgcttatta cgtttgtttg tattgatctt aaaacgcaga
    12901 aagcattagc tctggaaggg gaattgcgcg aaaagctgga gcagatggtt aagtaaacgt
    12961 tttgtggtgc cggatgctca agccgcatcc ggcgacaccc ggaataatta cttcaacccg
    13021 gttttctgct tcatcgctac catcaccgtc ggtttatcgg ccagataatg attcaaaccg
    13081 ttggcacgta aattacacgc cgcacaatga ccgcaaccgt cgcctttaat gccgttatag
    13141 caggtcagcg tttcgttacg gactaaatcc agcttgccgt aataatctgc cagcgcccag
    13201 gtttccgctt tatcaatcca catcagcggc gtttcaaaac gaatatcttt cgccatgccc
    13261 aaactgacgg catggtttag tgctttcaca aactcatcgc ggcaatccgg gtagccggag
    13321 aaatccgttt cgcagacgcc ggtaattacg gcttctgctt ttacctgata cgcatatatt
    13381 gccgccagcg tcaggaacaa aatattacgc cctgggacaa acgtattcgg gataccatcg
    13441 gcttcaggtt cataatcagg caccggaatg ctgtcacgcg tcaggctgct gaccgccagc
    13501 tcgttgagca gcgttacatc aagcacctta tgcgcgcgtg cccccagttt cagcgccagt
    13561 tcgcgtgcca cgtcgatttc tgcgcgatgg cgttgaccgt aatcgaacgt cacgcaatgg
    13621 acttcatcat attgttgtaa tgcctgcacc agacaggtgg tggaatcctg gcctccactg
    13681 aacacaacga cagcacgttt catagataat cctgcttgaa caataaaagc gttatggtaa
    13741 cgcctgcgat taacccggac cagctattca ttgcgacggc gcgggaagcc aggcttcggt
    13801 aaattcaaac cagccgcgcg gtgtcagtcg cacaccgttc acgccgggag gggcgctaat
    13861 gcgatagtga tagttgaaca gcggcgtcag cgtcgcatct gccattaact ggctaaaaac
    13921 cgctttcagg gcattaaatc ggttttcctc atcaggcatt acttgcactg cgtccagtgt
    13981 cgattgcaaa tgtgcatatg ctggagcgtc gaaaacatgt ggccacagtg gatcgcaacg
    14041 cagccattgc tccagggtat attccggtgc ttcgccaatt aatctgtcgc ccatcatgag
    14101 gtctgcgtgt gccagtaggg tcgtgtcgtc ccagtttttt gcgttatgaa aaataattgt
    14161 gagttcacag ccttccgctg ccagtgtcgc ctgtaggcgt tctgccatgg tatgaagttc
    14221 tatcggtagg tgataaacca gcgtcaaggt tttcggtagt ttaacttcat ccggtacctg
    14281 ccaatgcgga atagtccagc caggcagtaa tgcatgactg gcggtgatca ggttttctcc
    14341 gacttctaac gtttgtaata aaccggattg atgaataatg gagattactt ttcgcgcctg
    14401 ccagagggac agtcggagac ttttgcgcaa tgttaaatag caaaaaccta aactgatgcc
    14461 gctactgacc tggctgaccc gttgcagctc ctccggtttg ccaatggtga tttgcacggg
    14521 atgccgacaa ctggttccca aatctttttc gaaaagcggc ggagttatcc agtactcaac
    14581 cgctttaagc agcggatgac gtaaatggta ataatcatgg ctctccaggc gcaccagttc
    14641 cgctgtgaat tgtgttaagc gaaaaggacc tgtgccgatc agtgggaatt gcggatgcgc
    14701 cagatggctg caatagctcg ccagtcggtg cgcaagccag taatcggggc gatgtaaaaa
    14761 gaaggtcaga cactgcggat gggtgacttc aatacgcttc acgctaataa ataattgatc
    14821 cagtgctggc agttgtaaca gcatcaataa tcgctggtgt aagtgtgagg cttttactgc
    14881 atcgccgtta tgccagtgta gcgttgaacg aagataaaag tcccagcgta acccgtcagt
    14941 agaggtttcc cagtgatgtg ctaaatcgcc aatcgggcgc tgagtattat tatcgaagcg
    15001 ggtcaggccg gaaaatatct gcccggccag atgctgctcg gcacggccgg gcaaaaagcc
    15061 cggttgtagc ggttcgagcg ggcgatagta gggaatacgc aacgtgggcg tatcgttttg
    15121 ccattgtccg cccataaacg gctgtaacag agtgcgcaac tcacctgggg ccagttgcgc
    15181 cagctccagc acatcttgct gctttccggt ttccagcgcc tgttccatca tcgcattgcg
    15241 tagcgattct ggcgtgacca gaaagcgtag ttgaccgcgc tttccgcgtc ctgactgcgc
    15301 ctgccactcc agccatcccg cctcctgtgc ctgacgcaac accgtacgaa catggcgttc
    15361 gctgcaaaaa cagcgttcgg ccagttcgct gacggtgacg gtttgcggct ttccggcgga
    15421 aggttgccac agacgttgat actggttaag acggttgagc agtcgcatat aaacccggaa
    15481 caatattatt taactattca ctattacttc cgtatatatc aggtgatact caatcaccat
    15541 taaccgtgtc acagagtgga gaaagaaatg gctcgtctgg cagcatttga tatggatggc
    15601 actttattga tgcctgacca tcatttaggt gagaaaaccc tctctacttt ggcgcggctg
    15661 cgtgaacgcg acattaccct cacttttgcc acggggcgtc atgcgctgga gatgcagcat
    15721 attctcgggg cgctatcgct ggatgcatat ttgattactg gcaacggaac gcgcgtgcat
    15781 tctctggaag gtgaactttt acatcgtgat gatttacctg cggatgtcgc ggagctggtg
    15841 ctgtatcagc aatgggatac ccgagcaagc atgcatatct tcaatgacga tggttggttt
    15901 accgggaaag agatccctgc gttgttgcag gcatttgtct atagcggttt tcgttatcag
    15961 ataatcgatg tcaaaaaaat gccactcggc agcgtcacta agatctgctt ctgtggcgat
    16021 cacaacgatc ttacacgctt gcagatccag ctatacgaag cattaggcga gcgtgcacat
    16081 ttatgttttt ccgccacgga ttgcctcgaa gtgctgccgg tgggttgcaa taaaggcgct
    16141 gcattgacgg tgctgaccca acatttaggt ttatcgttac gcgattgcat ggcctttggt
    16201 gatgcgatga acgatcgcga aatgttaggc agcgtcggta gcggatttat tatgggcaat
    16261 gcgatgccgc aactgcgcgc ggagctcccg catttaccgg tgattggaca ttgccgaaat
    16321 caggctgtct ctcactattt gacgcactgg ctggactatc cacatctacc ttattccccc
    16381 gaataacgag atcccttcca gcaccgggca attgcccggt tttttttgcg ttgaatttgt
    16441 cattttgtgc cgtggtgctt aaaccgcaca gaataaattg tcgcgatttc acctttaaaa
    16501 tagaattaaa agagaaaata attctctgtg gaagggctat gttagataaa attgaccgta
    16561 agctgctggc cttgctacag caggattgca ccctctcttt gcaggcactg gctgaagccg
    16621 ttaatctgac aaccaccccc tgctggaagc gtctgaaacg gctggaggac gacggtatcc
    16681 ttatcggcaa agtcgccctg ctggatccgg aaaaaatagg cctcggcctg accgcctttg
    16741 tgctgataaa aacgcaacat cacagcagcg aatggtattg ccgctttgtc acggtggtta
    16801 ccgaaatgcc agaagtgctg gggttctggc gcatggctgg tgaatacgat tatctgatgc
    16861 gcgtccaggt tgccgacatg aaacgctacg acgagtttta taagcgtctg gtaaacagtg
    16921 taccggggct gtcggacgtc acttccagct tcgcgatgga acagattaaa tacaccactt
    16981 ctttacccat cgaataaata tccagaatca agtcaggaca caacgcgtgc gattatttgc
    17041 tcaattaagc tggtatttcc gtcgggaatg gcgtcgctat ctcggggctg tcgccttgct
    17101 tgtcattatc gcgatgctgc aactggttcc gccaaaagtg gttggtattg ttgttgatgg
    17161 cgtgacagaa caacacttta ctaccgggca gatcctgatg tggatcgcca ccatggtgct
    17221 gattgccgtt gtggtttatc tcctgcgtta cgtctggcgc gtattgctgt ttggtgcgtc
    17281 ttatcaactg gctgttgaac tgcgtgaaga ttattaccgc cagctaagcc ggcagcatcc
    17341 tgagttttac ctgcgtcatc gcacgggcga ccttatggcc cgtgcgacaa atgacgtcga
    17401 tcgcgtcgtg tttgccgccg gagaaggggt gctgacgctg gtggattcac tggtaatggg
    17461 ctgcgctgtg ttgattatga tgtctacgca aattagctgg cagttgacct tatttgccct
    17521 gttgccgatg ccagtgatgg cgatcatgat caagcgcaac ggcgatgcct tgcatgaacg
    17581 ctttaagctg gcacaggcgg cgttttccag tcttaatgac cgcacccagg aaagcctcac
    17641 cagtatccgc atgatcaaag cctttggtct ggaagatcgt cagtcggcgt tatttgccgc
    17701 ggatgccgaa gataccggca aaaaaaacat gcgggtggcg cgtattgatg ctcgtttcga
    17761 cccgaccatc tatatcgcga ttggtatggc gaacttgctg gcgattggcg gcggtagttg
    17821 gatggtggta cagggcagtt taacgctggg ccagctcacc agttttatga tgtatttagg
    17881 tctgatgatt tggccaatgc tggcgctggc atggatgttt aacattgtgg aacgtggcag
    17941 cgcggcgtat agccgaattc gtaccatgct ggccgaagcg ccagtcgtta ttgatggtag
    18001 tgataaggtg ccagaaggtc gtggtgaact ggatgtgaat attcgccagt ttacgtatcc
    18061 gcagactgac catcctgcgc tggaaaacgt caatttcgcc ctgaaacccg gtcagatggt
    18121 aggtatttgc gggccgactg gttccggcaa aagtaccctg ttgtcgctca ttcagcgtca
    18181 tttcgacgtc agcgaggggg atatccgctt tcatgatatt cctctgacga agttacaact
    18241 cgatagctgg cgtagccgcc tggcggtagt tagccagaca ccgttccttt tttccgacac
    18301 cgtagcgaac aacatcgcgt tgggttgccc gaatgccacc cagcaagaga ttgagcatgt
    18361 cgcgcggtta gccagcgtgc atgacgatat tttgcgtctg ccgcaaggtt acgacacaga
    18421 agtgggcgag cgcggcgtga tgctttctgg cggacaaaaa cagcgtatct ccattgctcg
    18481 agcgttatta gtcaacgcgg aaatcctcat ccttgatgat gcgctttcag cggtggacgg
    18541 acgtacagag caccagatcc tgcataacct gcgtcagtgg gggcagggaa gaacggtaat
    18601 catcagtgcc catcgccttt ctgcactgac ggaagccagt gaaattattg tgatgcagca
    18661 cgggcatatc gcccagcgtg gcaatcatga tgtgctggta caacaaagcg gctggtatcg
    18721 cgatatgtat cgctatcaac aactggaggc ggcgctcgac gacgctccgg aaattcgcga
    18781 ggaggccatc gatgcgtagt tttagccaac tgtggccgac tctcaagcgc ctgttagcgt
    18841 acggttcacc gtggcgtaaa ccgttgggga ttgcggtcct gatgatgtgg gttgcggcgg
    18901 cggcagaagt cagtgggccg ctgcttatca gctattttat cgacaatatg gtagcgaaaa
    18961 ataacctgcc gttgaaagtg gttgcagggc tggcggcggc gtatgttggg ctgcaactgt
    19021 ttgcagccgg gctacattac gcgcagtcgc tgctgtttaa tcgggcggca gtaggcgtag
    19081 tgcaacagtt gcgtaccgac gtgatggatg ctgcgttacg ccagccatta agcgagtttg
    19141 atacccaacc cgtcgggcag gtgatttccc gcgtcactaa tgacactgaa gtgatccgcg
    19201 atctctacgt taccgtagtg gcaactgtcc tgcgtagcgc cgcactggtg ggcgcgatgc
    19261 tggtggcgat gttcagcctc gactggcgaa tggcgctggt ggcgataatg attttcccgg
    19321 tggtgctggt ggtaatggtg atatatcagc gttacagcac gccgattgtc cgtcgtgtgc
    19381 gcgcttatct ggcggatatc aacgacggct ttaacgaaat catcaatggc atgagcgtta
    19441 tccagcagtt ccgtcagcag gcgcgatttg gcgaacgtat gggtgaggcc agtcgttcac
    19501 actatatggc gaggatgcaa accctgcgcc tcgacggttt tctgctgcgt ccgctgctga
    19561 gtctgttttc atcgctcatt ctttgtggct tgttgatgct gtttggcttc tccgccagcg
    19621 gcaccattga agtgggcgtg ttgtatgcgt tcatcagtta tttggggcga cttaacgaac
    19681 ccttaatcga actgaccacg cagcaggcga tgctgcaaca ggctgttgtt gctggtgagc
    19741 gcgtttttga gctgatggac ggaccgcgcc agcaatatgg caatgatgat cgcccgttgc
    19801 agagcggcac catcgaagtc gataacgtgt catttgctta tcgcgatgac aatctggtgc
    19861 taaagaacat taatctctct gtgccttcgc gcaactttgt ggcgctggtc gggcataccg
    19921 gcagtggcaa aagcaccctc gccagtttat tgatgggcta ttacccgcta acggaaggtg
    19981 agattcgcct tgatggtcgt ccattaagta cgctaagtca cagcgcgctg cgccagggcg
    20041 tggcaatggt gcagcaagat ccggtggtgc tggcggatac cttcctcgcc aacgtgacgc
    20101 tggggcgtga tatctctgaa gaacgcgtct ggcaggcgct ggaaaccgtg cagctggcgg
    20161 agctggcgcg tagcatgagt gatggtattt acacgctgct tggcgagcag gggaataatc
    20221 tctcagtcgg gcaaaagcaa ctgctggcac tggcgcgcgt gctggtggag acgccgcaaa
    20281 tcctgatcct tgatgaggca accgccagca ttgactccgg gactgaacag gcgattcaac
    20341 atgctctggc ggcggtgcgt gaacatacta cgcttgtggt gattgctcac cgcttatcga
    20401 ctattgttga tgccgacacc attctggtgc ttcatcgtgg gcaagccgtg gagcagggca
    20461 ctcaccagca actgctggcg gcccagggac gatactggca gatgtatcaa ctgcaacttg
    20521 cgggcgaaga gctggcagcc agcgtgcgtg aagaggaatc attgagcgcc tgaatagcgc
    20581 aatatttcat cgttggtgca aaaatgtaac gcactgtgca ctgtcatagt gcgttttcat
    20641 tttcaatctt cttaacttcc tgctctcttt ctcgtttttc atttctggca caccgcttgc
    20701 aataccttct tcgtgtagca gaaccattac cgaattctga ccggagggga tctatgaagc
    20761 tggtgaccgt gataatcaaa ccattcaagc tggaagacgt tcgtgaagcg ttatcttcca
    20821 ttggtattca gggcctgacc gtcaccgaag tgaaaggttt tgggcgtcag aaagggcatg
    20881 ccgagctgta ccggggggcg gaatacagcg tcaacttcct gccaaaagta aaaattgatg
    20941 tggcgattgc tgatgaccaa ctcgatgaag tgatcgatat cgtcagcaaa gcggcttaca
    21001 ccggaaaaat tggcgacggc aaaatcttcg tcgctgaatt gcaacgcgtc attcgtattc
    21061 gtaccggcga agccgacgaa gcggcgctgt aatctctggc acacagcaac aggaacgaaa
    21121 aatgaagata gcaacgataa aaactgggct tacttcactg gcgatgctgc cgggactggt
    21181 aatggctgca cctgcggtgg ccgataaagc cgacaatgca tttatgatga tttgtactgc
    21241 gctggtgctg tttatgacta ttccggggat tgccctgttt tatggagggc tgattcgtgg
    21301 caaaaacgtg ctgtcgatgc tgacgcaggt gaccgtgacg tttgcactgg tctgtattct
    21361 ctgggtggtt tacggctact cgctggcgtt tggcgaaggc aacaacttct tcggcaacat
    21421 taacgggttg atgctgaaaa acattgaact gacggcggtg atgggcagca tttatcagta
    21481 tatccacgtg gcgtttcagg gatcgtttgc ctgcattacc gtaggcttga tagttggggc
    21541 gctggcggaa cgaatccgct tctcagctgt gttgattttt gtggtggtat ggctgacgct
    21601 ctcttacatt ccgattgcgc atatggtctg gggcggcggt ttgctggctt ctcacggtgc
    21661 gctggatttc gcgggtggca cggtagtgca cattaacgcc gcaatcgccg gtctggtggg
    21721 cgcgtatctg ataggcaaac gcgtgggctt cggcaaagag gcgtttaaac cgcacaacct
    21781 gccgatggtc ttcaccggga ctgccattct ctatatcggt tggtttggct ttaacgccgg
    21841 gtcagcgggc acggcgaatg aaatcgcggc actggcattt gtgaatactg tggtcgcaac
    21901 cgcggcggca attcttggct ggatcttcgg tgaatgggcg ctgcgtggta agccttcact
    21961 gctgggagcg tgttctggcg cgattgccgg tctggtcggc gtgacgccag cctgtggtta
    22021 cattggggtt ggcggcgcgt tgattatcgg cgtggtagct ggtctggcgg gcttgtgggg
    22081 cgttaccatg ctcaaacgct tgctgcgggt ggatgatccc tgcgatgtct tcggtgtgca
    22141 cggcgtttgt ggtattgtcg gctgtatcat gaccgggatt tttgccgcca gctcgctggg
    22201 cggcgtgggc ttcgctgaag gtgtgacgat gggccatcag ttgctggtac agctggaaag
    22261 catcgccatt acgattgtct ggtctggtgt ggtggcattt atcggctaca aactggcgga
    22321 tttgacggtt ggtctgcgtg taccggaaga gcaggagcga gaagggctgg acgttaacag
    22381 ccacggcgag aatgcctata acgcgtaaca agcactgcaa aaaatagccg gacggttttt
    22441 acctccggct atttttttaa ttgtgattac gcatcacccc ttcctgaacg gtcgaggcaa
    22501 ccagcacgcc gtcctgggta taaaactcac cgcgcacaaa gccgcgtgcg ctggacgccg
    22561 aggtgctttc cacgctatac agcagccatt cattcaaatt aaacgggcga tggaaccaca
    22621 tggaatggtc aatggtggca atctgaatcc ccggttcgag aaaaccgatg ccgtgcggct
    22681 gtagagctac cggcaggaaa ttaagatcag aagcataacc gagcagatac tgatgaacgc
    22741 gcagatcatc cggcacgcta ccatttgcgc ggatccacac ctgacgatgc ggttctgcga
    22801 cgtgaccttt cagtgggtta tgaaactcca ccggacggac ttccagcgga cgatcgcaga
    22861 tgaatttatc tttcagcact ggcggcagca ggtgcgccag cgattgggcg atttgcgttt
    22921 ctgaaggcag gccatcaggc gctggcgcgg acggcattgt tttttgatgt tcgaaacccg
    22981 cttctggtgc ctggaaagag gcagtcatat aaaaaatcgg tttgccgttt tgaatagcag
    23041 aaacccggcg ggcgctgaag ctgttaccgt cacgcagcgt ttcgacatca taaataatcg
    23101 gcttcttact atcgccaggg cgaagaaagt agctgtgaaa agaatgtacc agccgctcgt
    23161 cagggacggt ctcttttgca gcatacaagg cctgacccac gacctggccg ccaaatacct
    23221 ggcgtaaacc taaatcttca ctctggccgc gaaagagtcc ttcctcaatt ttttccagat
    23281 ttaacaatgt cagtaaattt tttagcgcct gactcatata actctccagt aacaaagctg
    23341 ccgcagcaag ccaaagtgag ttgagtataa cgcaaatttg ccactggtcc gatgggtgca
    23401 atggtctgaa ttacgggcta attacaggca gaaatgcgtg atgtgtgcca cacttgttga
    23461 cgttactatt ttgttaacca ctcttccggc gaggaaagtt agcccgctgg tgcattgata
    23521 ataaggagaa gtgaatgaaa ctcgtgcaca tggccagtgg tttagcggtt gcgattgcgt
    23581 tggcggcttg cgcagataaa agcgcggata ttcagacgcc agcaccggct gcaaatacgt
    23641 ctatttcagc aacacaacaa cctgctatcc agcaaccgaa tgtctccggt accgtttgga
    23701 tccgtcagaa agtcgcactg ccgcctgatg ccgtgctgac cgtgacactt tctgacgcgt
    23761 cgttagccga tgcgccgtcg aaagtgctgg cgcagaaagc ggtgcgtacc gaaggtaaac
    23821 agtcaccatt cagctttgtt ctgccattta acccggtaga tgttcaaccg aacgcgcgta
    23881 ttctgttgag tgcggcgatt accgtgaatg acaaactggt atttattacc gataccgttc
    23941 agccggtgat caaccagggc ggaactaaag ccgacctgac attggtgccg gtgcagcaaa
    24001 ccgccgtgcc ggttcaggct agcggtggcg caacgactac cgtaccttca acttcaccaa
    24061 cccaggtgaa tccgtcttcg gcagttccag ctccaacgca atattaagcg aagttaaccc
    24121 tctccgcccg gagagggtta gtagttccag cgataaagct gcaagtcgat ttgcccgctt
    24181 cccgacacca tcacaccttc tgccagtaat gcctgtcgct gacgctgtaa atccggtccg
    24241 gtcagtgaaa tggtgccgtg gcgattaacc acccggtgcc agggcaaggt gctgccttcg
    24301 gggagacgct ttaacacacc gcctacctgg cgcgcggcgc ggggcgatcc cgccagtttc
    24361 gccacatcac cgtaagtggt gacatagcct tcgggaatag cggctacgat ttgccagacg
    24421 cgttggggaa atgaatcttc tttttccatc ttttcttcct gaggtaattt ttcagcataa
    24481 tctggaaaaa cgcccgagtg aagccgcatt gcgcaagaaa ccagcatctg gcacgcaatg
    24541 ggttgcaatt agccggggca gcagtgataa tgcgcctgcg cgttggttct caacgctctc
    24601 aatgggggct ctgttggttc tcccgcaacg ctactctgtt taccaggtca ggtccggaag
    24661 gaagcagcca aggcagatga cgtgtgtgcc gggatgtagc tggcagggcc cccacccatt
    24721 tctgcctccc accgtttcgt caaaaaatcc caacatggct aaactttaac cacaactgac
    24781 gtcgcaagaa ttgtctggct gcgcagtacg cttcggaggt atgtctgatg aagtatgttg
    24841 atggttttgt tgttgccgtt cctgccgata aaaaggatgc ctatcgggaa atggccgcta
    24901 aggtcgcgcc attatttaaa gagttcggtg cgcttcgtat tgtcgaatgc tgggccagcg
    24961 atgtaccgga tggtaaagtg accgattttc gtatggcggt gaaagcggaa gagaatgaag
    25021 aggttgtctt tagctggatt gaataccctt caaaagaggt ccgcgacgct gctaatcaaa
    25081 agatgatgtc ggacccacgg atgaaagagt tcggcgagtc catgccgttt gacggcaagc
    25141 gaatgatcta tggtggattc gaatcaatca tcgacgagta ggtagcgtga cgggctgtgc
    25201 ggcgtgccgt cagccctaaa attcacaaat gttgctcggc ccagcgtaag aaatcctctt
    25261 tcggtaatgc cttgctgtag agccagccct gaccgtagtg cacgccatgc tggcgtagcc
    25321 actcttcttg tttactggtt tcgattccct ccgcaaccat tttcagtttc agtgtttttg
    25381 ccatttcgat gatatgcggc gtgacatttt tatattccag cgcatcaacg aaagatttat
    25441 cgatcttcag aatgtcgaca tccagatcct gtaaatagct taaacttgaa taccctgtac
    25501 caaaatcatc gagataaatt tcatggcccg tctcccggta gcgagaaatt atcggggcgc
    25561 tggttttcgg atcggcaaac tcgcgttcag tgagttcaag cgcgatctgt ctgggattaa
    25621 cctgatagtg attgatcatt tcacgcagca attgcgggat tttttccgag gtgagcacgg
    25681 tggattcaag attgatcgaa atattctgct gagaatgctg acgcagccag tcgcccatat
    25741 cttcaaagac gcttcttata atcagtagcg tcaatggctc agaaaggccc gtttgctgtg
    25801 ccagcggaat aaaactatct ggtgacaacc aactaccgtc tgtctgcggc cagcgcgcca
    25861 gtgcctcagc accgacaatt ttgccattgg ctaaggagac aatcggctga tagtgcacgc
    25921 aaatatcacg attttcgata gcatcctgca gccgatgatg cggtgactgg atacggcgca
    25981 aaatacgcag cacaaacatc gctgccagca ggccaatcac caacccgagc ggtaaccaaa
    26041 taaagacttg ccgatgccag cctttctgca acattttcgt tgaagcccag gtgatgatcg
    26101 aaatattcat ctccggtaag ggcaggatat cgtaaatgat tccattattt tcgatatgct
    26161 caccgggtgt tttttgtagc ctggtaataa ttccctgagc aagttcatcg ctgctggtta
    26221 tgacaacgtt atgggcattg ccaataatgg cggcatcaat ttgccatgag ctatagggaa
    26281 tgacatcaat aaaggaagcg gggtcgatca tgacgacata atgtgccgtt cccatggcga
    26341 ccatgtaacg gataatgcct aaatcgttat gcgatgttaa ccagacacga taaccatctt
    26401 tcgaaatttt acctggctcg gggaaggtat cgggcgggct ttcatgctcc agagacgaac
    26461 attggggaac gttgttatcg atataaacca cttcctgaat atagcgataa ctgtaagata
    26521 cccgacgcat ttccatgaga tgggcttcgc tacaggcagc gccttgccat ctttccagct
    26581 cttgcagcgc atctttccct tgtgtcgcca ccttattggc ccgaatagcg acgcgggagg
    26641 aatacgtatt cagctcttca ataaatgacg tttctacctg ctgatgggcc agccagatgc
    26701 ttaagccgac aggcagcaat actgaaagaa tcagtactcc cgaaataagg ccgaccagat
    26761 gtcgtgttct caccagcata tccttattaa cacagtgtag ggaaagtata tctgattgtg
    26821 gaaaaaggct ggcaggagat aaggaggtag agctggtatt tcatgaagtg atgggtggaa
    26881 gcaatcgttg tcatttgacc aggcgtagag ctttacgcct ggtatgtacc cctaacttaa
    26941 gatgttgatt cggctcgagc gagggtaaaa atatcgtaaa aagatagttc acctttacgg
    27001 acgatcattt tttgcagttg ttctttatga acatccgcga cacgtggaga gttcaggatg
    27061 gcttcaacca gcgcggcagg tacaaagcgc gtgttgtacc attcgccgtt atagcaaacg
    27121 cggaaatcca gcacgtcgat atcttcatag cggtagcgtg ggtagacatc gaaaaagaag
    27181 aaaccattaa gcaggataaa taatacaacc agtagccaga cagagcctga aagcgtttcg
    27241 gattgcaaca tcaccgccag ggtagcaaaa aaagcaacgt acataccgat aaacagcccc
    27301 ggatgtttac ggataaaact gatactaaag cggggtttgt tgtcgcgttt ttcgcgggta
    27361 ttcagagact caatcgtttc ggtcagcagg cgttgtattt cggtcatcgt ttgccttcgt
    27421 gatatctgca actttccagg gaacacccta ttttaacgtg ggaatagtag aggggaagta
    27481 acagatttgc cacgattttg cataacagtt gagaaaagct ggttacaatt ttttaatgat
    27541 tctggctgga ttaccgccca cgacaacgtt gtccgggaca tcttttgtga caactgcacc
    27601 tgaggctacc acgacgttat caccaatggt cacaccaggg ttaatgactg cacgtccgcc
    27661 aatccagacg ttattaccga tggtgacggg tttccccagt tcagcaccgc tattacgtgc
    27721 tacagggtcg atgggatgtg ttgccgtgta gatatgaacg cctggtgcca acatacagtt
    27781 atcaccaatg cgaatagggc agacatcaag catcacgcaa tcgaagttgg cgaaaaaatt
    27841 attgccgaga aaaatgttat aaccatagtc acagcgaaac gttggctcaa tataagcctc
    27901 tgtcacctga ccgaataaat cagcaagaat ttgctggcgt aatgtgtgct cttccgccag
    27961 ggaatgattg tatcggtgaa taagccgacg agcgcgcagg cgatcgcgag ataacgcctc
    28021 atctgccgag tgatacaact caccagcaat catcttttct ttttctgtgc tcattactga
    28081 cctccggttg ctgaaaatgc ggcaacacag taagacgaaa agtcagtgaa gagaacgttc
    28141 ccattacatc tgtgtgatga agatcacata gggtatgtct tcatggcgaa aaaagtataa
    28201 aattcttaat aaacagccag ttatagctcc gaaagcgagt tatttattag cgaataaatt
    28261 tccatactga ggaagggatc ttgtcgtaca gtttattcat ggtcaattcg gcgaggcggt
    28321 gatctgcagc tgagtaaaat accgccagtt cattatctga taattcgtat ttatttttct
    28381 cgataacacg ctccagcgtg tcaattgtct gacaacgacg taaacgcatt aaataatcgg
    28441 ttttcgttaa aggtttttcg gacatacttc tacccatggt tgtaataatt ctaacaagat
    28501 aaactcgcag gattctcttt cgtcgcattg acaaaacaac ggaatagtcg attacctgac
    28561 ttccgccatt tctgaagatc ctgcatatta ataccataac tactgaacaa cataaaggtg
    28621 tcatccagat attcgtcgat ctgctcaatg agcttattgt cttcattata cttaattttg
    28681 taattaagtg cgaaggtcgc aatatgctca atcagttcat tcaactggag gttgatcgcc
    28741 gaggttgggt cgttaaccca gccatgattg ctttcttcaa ggtttgcaag gcagtcatga
    28801 tacagggttt cacagagaaa cttaagctgt gcgatatcat gtcttttggg tgagtattca
    28861 tccataacgc gtccccttct tagcggttga actaacggac acctttcggg atggaaaaaa
    28921 cttactgacc tggacttgcc ctctcgtttg ttagcttaac tataagccac tctttgtagg
    28981 ttttcatcgc attattacga aaaattacaa ttaggaagga agcgggacag gactcacttt
    29041 ttcatactag caccaacccg cagcaggtaa aagcagtttg ctcgaactgt tcataacatt
    29101 tcagaattat cgccagaaga aattggtgac actaatacca ggattgctct gaatatgacg
    29161 taataaccga ggaatgaata aagaattacc gcaaataatt aagaatagcc tctaaatggt
    29221 tatggattat taaatctatt agcgctactt aatataaatt aactaacaat cagtattttt
    29281 tatgaatttt atacgtggtt aatactggtt ttcgtatggg atcctgagtt ggtggttcaa
    29341 tacatcctta atgttcgtag gttatgcata aaaaaggccg cttgcgcggc cttagtgatt
    29401 acacgttgta tcaatgatga tcgacagtat ggttgtgctc gatatcttca ttcttgcggc
    29461 taaagcggcg gcgaaccacc acaaagaata ccggaacgaa gaagattgcc agtaccgttg
    29521 cggtcaccat cccgcccatt acaccggtac ctactgcgtt ctgcgcgccg gaaccagcac
    29581 cagtactgat aaccagcggc ataacgccga ggataaacgc caacgaggtc atcaggattg
    29641 ggcgtaaacg catccgcacc gcatcaagcg tcgcttcaat cagaccttta ccttctttat
    29701 ccatcaagtc tttggcgaat tcgacgataa gtatcgcgtt cttcgccgac aaaccaatgg
    29761 ttgtgagcag gcctacctgg aagtaaacgt cattggtcag gccacggaag gtggcagcca
    29821 gcaacgcacc gataaccccc agcggaacga ccagcataac ggagaacgga atcgaccagc
    29881 tctcgtacag cgcagccaga cacaggaaca cgacaatcaa cgaaatcgcg tacagtgaag
    29941 gtgcctggtt gccggagaga cgttcctgat aggacatccc cgtccagtca tagccaacac
    30001 cggtaggcag tttgctcgcc agttgttcca tcagctccat tgcttcaccg gtacttttac
    30061 ccggtgccgc ctggcctaag atttccatag atggcaggcc gttgtaacgt tccagacgcg
    30121 gcgaaccgta ctcccaacga gaagaggaga acgccgagaa cggcaccatc tgaccatcag
    30181 cagcacgaac ataccagtcg ccgatatcat ccggcagcat acggtatttc gcctctgaca
    30241 tgacgtaaac tttcttcaca cgaccgcggt cgataaagtc gttcacatag ctaccgcccc
    30301 atgcagcgcc cagagtggtg ttaatgtcgt tgatagaaac acccagcgcc tgcgcttttt
    30361 cctggtcgat atcaatctta aactgcgggg tatcttccag accgtttgga cgtacgctgg
    30421 tcaacatatc agggtgcttc gctgcttctg caagcaactg gttacgcgcc tgagtcagtt
    30481 tttcgtgacc aaggccagcc tggtcaatca gctcaaagtc aaagccggtt gcggtaccca
    30541 gttccacgat tgcgggcagg ttaaaggcga aaaccatcgc atctttgatt tgcgagaaag
    30601 cacgtgttgc acgcatggta atcgcttcaa ctttgttttc ttcgcccgga cgatcggccc
    30661 agtccttcaa ggaaacgaac gcaataccgg tattctgacc acgtcccgca aagccgaagc
    30721 cgttaacggc gaacaccgac tcaacgttgt tcttttcttt ggtcagatag taattcgtta
    30781 cctcattgag cactttctgc gtacgttcct gcgttgcacc tgctggcagc tgaaccatgg
    30841 tcataaatac gccctggtcc tcatctggca agaaggagct tggcagacgc acgaacagat
    30901 aggccatgcc gaccacgatg atcagataca gcaccaggta acgccccgta ctgcgtagaa
    30961 taccgcctac gctgtcggtg tagtggtgcg tgctcttctc gaacatgcgg ttaaaccagc
    31021 cgaagaagcc ttttttacct tccccgtgat cgcctttggc aatcggtttc agcatggtgg
    31081 cacaaagagc cggagtcagg atcaacgcca ccagtaccga cagcgccatt gctgaaacaa
    31141 tggtaataga gaactgacga tagatagcac cagtagaacc gccaaagaag gccatcggta
    31201 cgaataccgc cgacagtacc atcgcgatac cgaccagagc gccctgaatc tgccccatcg
    31261 acttacgggt ggcttctttt ggtggcaaac cttcttccgc catcacacgc tcaacgtttt
    31321 ctaccacaac gatggcgtca tccaccaaca ggccgatggc gagcaccatc ccgaacattg
    31381 ttagcgtgtt tatcgagaag ccaaaggcgg caaggacggc aaaggtcccg agcaatacca
    31441 ccggtacggc aatggtcgga atcaacgtcg cgcggaagtt ctgcaagaac agatacatta
    31501 ccaggaacac gaggatgatc gcttcgacca gcgttttaac cacttcgtga atagagattt
    31561 tcacgaacgg tgtggtgtca tacgggtaaa caattttcag acccgacggg aagaacggtt
    31621 ccatcttcgc cagttcagca cggattgccg cagcggtatc cagcgcgttc gcaccggtcg
    31681 ccagcttgat ccccagaccg gaagccggtt ggccgttaaa ctctgcgatg atgtcgtagt
    31741 tctcaccacc cagctcaatt ttcgccacat cacgcagcag cacgcgggaa ccatcctgat
    31801 tcactttcag caggattttg ccgaactctt cagtagaggt cagacgcgtc tgagcaataa
    31861 tagaggcgtt aagctgttgg cctttcaccg gcggcgtacc accgagctga ccagctgcaa
    31921 cctgggcgtt ctgtgctttg atggcggtaa tgacatcaac cggcgttagc tggaatttgt
    31981 tcagttcatt cgggttcatc cagatacgca tcgcgtactg tgaaccgaac aactgaacgt
    32041 cacccacgcc cgacgtacgg ctgatggcat ctttcatatt cgccgccacg tagtcggaga
    32101 tatcctcctg cgtcatggtg ccatcggtgt tgataacgcc gacaaccatc aggaagctgc
    32161 tggatgattt ctcaacgctc accccttgct gctgtacttc ttgcggcagc aacggcatcg
    32221 ccagctgcag tttgttctgc acctgaacct gcgcgatatc cgcatcagta ccagactcaa
    32281 aggtcagggt gatctgcacg gtacccgtgg agtcactgtt agaggacatg tacatcaggt
    32341 tatcgatacc gttcatattc tgttcgataa cctgtgtcac cgtgtcctgc actgttttcg
    32401 catcagcgcc agggtaggag gcggagatcg ttactgccgg cggtgcaatc gtaggatatt
    32461 gcgccaccgg cagtttgagg atcgccagcc cccctgccaa catgatgata atggcgatca
    32521 cccacgcaaa aattgggcga tcgataaaga aattaggcat gtcttaacgg ctcctgttta
    32581 agttaagact tggactgttc aggctgagca ccgcttgcgg cttgctggtt attatcagcg
    32641 gtaacttctt gtgcttttac ctggacacca ggacgcactt tctgcagccc acttattact
    32701 acgcgatcgc ctgctttcag accttctgtc accagccact tatcgccgat agcctggctt
    32761 gcaacgatcg gacgggtttc cactttgtca tccgcgccaa ccaccagtac ggtggcatcg
    32821 ccacgtggcg tacgggttac gccctgttgc gggactaaaa tagcgtttgg attaagccct
    32881 tcttccagac gtgcacgcac gaacataccc ggcagcagag tgtgatccgg gttcgggaag
    32941 atagcgcgta gggtgataga cccagtggtc tgatcaacgg taacgtcaga gaattccagc
    33001 gtaccgtcct gcgggaactt aatgccgtca ctggtgatca gcgacacttt ggctttgccg
    33061 ttctcttgtt tcagcgtgcc attcgccagt tcctgtttca ggcgcaggaa gtcgttgctg
    33121 gactgggtca catcaacgta gatcggatca agttgctgca cggttgccag cgcagtcgcc
    33181 tgaccgttct gtaccaatgc gccttccgtc acgtttgact taccaatgcg accactaatc
    33241 ggagaggtga ctttggtgta agccagattg attcgcgcag tttcaacggc agctttcgcc
    33301 gcagttaccg cagcattcgc ctgttgcgca tcagccagag cctgatcgta ctcttgttta
    33361 ctgatgtact gagtaccgag caatttctga taacgattca ccgtcaattg cgcgatattg
    33421 gctgcagcct gggctttcgc cagatcacct ttcgcactgt cgtatgccgc ctgataggtc
    33481 gcaggatcaa tctgatagag agagacacct gcttcgatgt cgctaccttc tttgaaatta
    33541 cgcttcagga taatcccgct aacttgagga cgaacttctg cgatccggta ggcactggtg
    33601 cgacccggaa gctcggttgt gatctgcaga ggttcagttt tgactgttac tacgccaacg
    33661 gcgggcatct gctggccacc ttgttgggcc tgtttgtcgt cacatcctgt tagggctaag
    33721 ctgcctgaga gcatcagaac gaccgccaga ggcgtaaacc ctctgttttt gttcatatgt
    33781 aaacctcgag tgtccgattt caaattggtc aatggtcaaa agttaataaa cccattgctg
    33841 cgtttatatt atcgtcgtgc tatggtacat acattcacaa atgtatgtaa atctaacgcc
    33901 tgtaaattca cgaacatatg gcacgaaaaa ccaaacaaga agcgcaagaa acgcgccaac
    33961 acatcctcga tgtggctcta cgtcttttct cacagcaggg ggtatcatcc acctcgctgg
    34021 gtgagattgc aaaagcagct ggcgttacgc gcggtgcaat ctactggcat tttaaagaca
    34081 agtcggattt gttcagtgag atctgggaac tgtcagaatc caatattggt gaactcgagc
    34141 ttgagtatca ggcaaaattc cctggcgatc cactctcagt attaagagag atattaattc
    34201 atgttcttga gtccacggtg acagaagaac ggcgtcgatt attgatggag attatattcc
    34261 acaaatgcga atttgtcgga gaaatggctg ttgtgcaaca ggcacaacgt aatctctgtc
    34321 tggaaagtta tgaccgtata gaacaaacgt taaaacattg tattgaagcg aaaatgttgc
    34381 ctgcggattt aatgacgcgt cgcgcagcaa ttattatgcg cggctatatt tccggcctga
    34441 tggaaaactg gctctttgcc ccgcaatctt ttgatcttaa aaaagaagcc cgcgattacg
    34501 tcgccatctt actggagatg tatctcctgt gccccacgct tcgtaaccct gccactaacg
    34561 aataaccctg aatctgactc caggaatttt cctggacatt ttcgtcgttg ctattctggt
    34621 tcactgcgtc gtgatattct tgcggtttga ctttttcagg tcgttcttca ggttcagaaa
    34681 ccttcattca tcatgactat gttccagtat tacaaacgat cacggcattt tgttttttca
    34741 gcatttattg cttttgtttt tgtcttgtta tgccagaaca cggcgtttgc acgggcgtca
    34801 tcgaatggtg atttaccgac aaaagcggac ctgcaggcac aacttgactc actgaataaa
    34861 caaaaagatc tttctgctca ggacaaactg gtgcagcagg atctgacaga tacattagcc
    34921 accctcgata aaatcgatcg cgtaaaagaa gagacagttc agctacggca aaaagtcgct
    34981 gaagcgccgg aaaaaatgcg ccaggcgacc gcggcgttaa cagcacttag tgatgtcgat
    35041 aacgacgaag aaacgcgcaa aattctgagc gcgctttcgt tgcgccagct ggaaactcgc
    35101 gttgcccagg cgctggacga tttgcaaaac gcacaaaatg atctggcgtc ttataacagc
    35161 caactggttt cgttacagac gcagcccgaa cgcgtgcaaa atgcgatgta taacgcttcg
    35221 cagcagctgc aacaaattcg cagtcgtctg gatgggactg atgtcggcga gactgcctta
    35281 cgtcccagcc agaaagtgtt aatgcaggtc cagcaggctt tgctgaatgc cgagattgac
    35341 cagcagcgta aaagcctgga agggaacact gtcttgcagg ataccttgca aaagcagcgt
    35401 gattacgtga cggcgaacag cgcccgtctg gagcaccagt tacaactgtt gcaagaagcg
    35461 gtaaacagca agcgcctgac tttaaccgaa aaaacggcgc aggaagctgt ctccccggat
    35521 gaagccgcgc gtattcaggc taatccgctg gtgaagcagg aactggaaat taaccagcag
    35581 ttaagtcagc gtctgattac cgcgactcaa aacggtaatc agttgatgca gcaaaacatt
    35641 aaagtcaaaa actggctgga gcgtgcgctg caatcggaac gtaatattaa ggagcagatt
    35701 gccgttctga agggcagtct gctgttgtca cgtatcctgt accagcaaca acaaacactc
    35761 ccctcggcag atgaactgga aaacatgacc aaccgcatcg cggatttgcg tctcgaacag
    35821 tttgaagtca accagcagcg tgacgcgctg ttccagagcg atgcgttcgt cagcaaactg
    35881 gaagaaggtc acaccaacga agtcaacagc gaagttcacg atgcattatt gcaagtagtt
    35941 gatatgcgtc gcgaattgct ggatcagctt aacaaacagt tgggtaatca gctgatgatg
    36001 gccattaacc tgcaaattaa ccagcagcag ttaatgagcg tatcgaaaaa tctgaaatcc
    36061 atcctgacgc agcaaatttt ttgggtgaac agtaaccgcc cgatggactg ggactggatt
    36121 aaagcgttcc cgcaaacgct gaaagatgaa tttaagtcga tgaaaatcac ggtgaactgg
    36181 gaaaaagcct ggcccgccgt attcatcgcc ttcctcgctg gtttgccgct gctgttgatt
    36241 gccgggctga tccactggcg tctgggctgg ctgaaagcgt atcaacaaaa actggcttcc
    36301 gctgtgggtt ccctgcgtaa cgacagccag ctcaacacac caaaagcgat ccttatcgac
    36361 ctgatccgcg cactgccggt gtgcctgatt attctcgctg tcggcctgat tctgctgact
    36421 atgcagctca atatcagcga gttattgtgg tcgttcagca aaaaactggc gattttctgg
    36481 ctggtgtttg gcctgtgctg gaaagtgctg gagaaaaatg gtgttgccgt gcgtcacttt
    36541 ggtatgccgg aacagcagac cagccactgg cgtcggcaaa ttgtccgcat cagcctcgcg
    36601 ttgctgccga tccatttctg gtctgtggtg gcagaactct ccccgctgca tctgatggat
    36661 gatgtgctgg ggcaggcgat gattttcttc aacctgctgc tgattgcctt cctggtgtgg
    36721 ccgatgtgcc gcgaaagctg gcgtgataaa gagtcgcaca ccatgcgact ggtcaccatt
    36781 accgtgctgt cgattatccc gattgcgttg atggtgctga ccgccacggg ttacttctac
    36841 accacgctgc gcctctccgg gcgttggatt gaaaccgttt atttggtgat catctggaac
    36901 ctgctgtacc agacggtatt gcgcggctta agcgtagcgg cgcggcgtat cgcctggcgc
    36961 cgtgcactgg cgcgtcggca gaatctggtg aaagagggcg cagaaggtgc cgaaccgccg
    37021 gaagaaccca ccattgcgct ggagcaggtt aaccagcaga cgctgcgtat taccatgttg
    37081 ctgatgtttg cgctgttcgg tgtcatgttc tgggcaattt ggtctgattt gatcaccgtg
    37141 ttcagctatc tcgacagcat tacgctctgg cattacaacg ctactgaagc tggtgctgcg
    37201 gtggtgaaaa acgtcaccat gggcagtctg ttgtttgcga ttatcgcctc aatggtggcc
    37261 tgggcgttga ttcgcaacct gcctggtttg ctggaagtgc tggtgctctc gcgactgaat
    37321 atgcgccagg gcgcgtcgta tgccattact accatcctta actacatcat tattgccgtt
    37381 ggtgcgatga cggtgttcgg atcgctgggc gtctcgtggg ataaactcca gtggctggcg
    37441 gcagcattat ccgtaggtct tggttttggt ttacaagaga tcttcggtaa cttcgtttcc
    37501 ggtttgatca ttctgttcga acgtccggtg cgcattggcg acacggtaac cattggtagc
    37561 ttctcgggta cggtcagcaa gatccgtatt cgtgcgacaa cgattaccga tttcgatcgc
    37621 aaagaagtga ttatcccgaa caaagcgttt gttaccgagc gtctgatcaa ctggtcgttg
    37681 actgacacga ctacgcgtct ggtgatccgt ctcggcgtag cctatggctc cgatctggaa
    37741 aaagtgcgta aagtgttgct gaaggcggcg actgagcacc caagggtgat gcacgaacca
    37801 atgccggaag tcttctttac ggcatttggt gccagcacgt tggatcatga gctgcgtctg
    37861 tatgtgcgtg aactgcgtga ccgtagccgt actgtcgatg agctgaaccg tactattgat
    37921 cagttgtgcc gtgaaaacga catcaacatt gcctttaacc agcttgaagt gcatctgcac
    37981 aacgagaagg gcgatgaagt gacggaagtg aaacgcgact acaaaggcga cgatccgacg
    38041 ccagcggtag ggtaaggaca ataaagggcg acagtgatgc cgccctttat tttttactcg
    38101 ctttgtgaag tctcatcgct ctgtaacttc ttttcataat cgcgttttac aatatccagc
    38161 gcgcgcagca cggtgcgggc tgggatctga ttttcttcca gcagcacaat caaatcgacg
    38221 gccagtttga catcgtcagg ggcattttcc agtgacatac tctctccatt gctagcgggt
    38281 taaacgcgct aacctgtttt cgattttttc cagcgcatgg cggcaacgtg ccaggcgcgc
    38341 ttcataggct tccacttcac gatgcagcgt ctgctgttcc acgagatcgg tcgctcgcgc
    38401 cagacgggct ctacgttcgg caaccatctc acgtagccgc cgctcaaaat cctgatgctg
    38461 aatacgttta cgctgccagc gggcaatttt cggtggtgcg ctatcccact cgcgtagcga
    38521 ccaggctgtg gcttcacgcg ctatagcctc cagttgcgcc gccagatgtt ctgccagcca
    38581 ggcgacttgc ggcagctgtt gctgctcaac cgcatgacga agcgcagcca ggttatcgcc
    38641 cgactcatcg agacaagcct gtagtgttgt cgcacgggtc tgaaaaaggt gcctgtcaaa
    38701 acgggcactt agcgtggcaa attgcgctac cggggcacaa cgctgacgca gcgtggcgag
    38761 ctgtccttcc agtttttcca gtagcagggc ggttttcacg ataagacctc aaggaaaatg
    38821 ataattgtta tgctaaagta gccactctgt aagctgacaa cactcaatat gcaacgaatc
    38881 attttaatca tcattggctg gctggcggta gtgctgggta cgctgggcgt ggtattaccg
    38941 gtattaccga cgacgccgtt tatcctgctg gcggcctggt gctttgcccg ttcttccccg
    39001 cgctttcacg cctggttgct gtaccgctca tggtttggca gctatctacg tttctggcag
    39061 aaacatcatg cgatgccgcg cggcgcaaaa ccacgggcga ttttgcttat tttgattacg
    39121 tttacgattt cgctgtggtt cgtccagatg ccgtgggtgc gcatcatgtt gctggtaatt
    39181 ctcgcctgtt tgctttttta tatgtggcga attccggtga ttgatgaaaa gcaagaaaag
    39241 cactgaagca caacaatcgc agttgcaatt attgcgtaca gccagtacat tctggcgttt
    39301 tcgagcacag gcgcaggcgg tcaaaggtta aacaactgtt acttttgata cgttcaaaac
    39361 gcgccgtgag taccaccgta acaagcaggc atacacttat gaccgcgact gcacagcagc
    39421 ttgagtatct caaaaatagc atcaaaagca ttcaggacta cccaaaaccc ggcattcttt
    39481 tccgcgatgt caccagctta ctggaagacc cgaaagctta cgctctcagc atcgacttgc
    39541 tggttgagcg ttacaaaaat gcgggcatta ccaaagtggt cggcaccgaa gcgcgtggct
    39601 tcttgtttgg cgctccggta gctctgggtc tgggcgttgg ctttgtaccg gtccgtaaac
    39661 cgggcaaact gccgcgtgaa accatcagtg aaacttacga cctggaatac ggcaccgatc
    39721 aactggaaat ccacgttgat gccatcaaac cgggcgacaa agttctggtg gtggacgatc
    39781 tgctggcaac cggcggcact atcgaagcga ccgttaaact gatccgtcgt ctgggtggtg
    39841 aagtagctga cgctgcgttc attatcaacc tgttcgatct cggcggtgaa cagcgtctcg
    39901 aaaaacaggg cattaccagc tacagccttg tcccgttccc gggccattaa ttatcgtcag
    39961 tcttgcgctg ctcacgctac gggcagcaca agatgtgcat tcagcctcgc cgttctgacg
    40021 gggctgtgtt agcattaccc cttcgtgaat ccaccttcca gcgtttcaga gcctgccaat
    40081 gagttatcag gtcttagccc gaaaatggcg cccacaaacc tttgctgacg tcgtcggcca
    40141 ggaacatgtg ctgaccgcac tggcgaacgg cttgtcgtta gggcgtattc atcatgctta
    40201 tcttttttcc ggcacccgtg gcgtcggaaa aacctctatc gcccgactgc tggcgaaggg
    40261 gctaaactgc gaaaccggca ttaccgcgac gccgtgcggc gtgtgcgata actgtcgtga
    40321 aatcgagcag gggcgctttg tcgatctgat tgaaatcgac gccgcctcgc gtaccaaagt
    40381 tgaagatacc cgcgacctgc tggataacgt ccagtacgct ccggcgcgtg gtcgtttcaa
    40441 agtttatctc atcgacgaag tgcatatgct gtcgcgtcac agctttaacg cactgttaaa
    40501 aacccttgaa gagccgccag agcacgttaa gtttttgctg gcgacgaccg atccgcagaa
    40561 attgccggtg acgattttgt cacgctgtct gcaatttcat ctcaaggcgc tggatgtcga
    40621 gcaaattcgc catcagcttg agcacatcct caacgaagaa catatcgctc acgagccgcg
    40681 ggcgctgcaa ttgttggcac gcgccgctga aggcagcctg cgagatgcct taagtctgac
    40741 cgaccaggcg attgccagcg gtgacggcca ggtttcaacc cagggggtca gtgcgatgct
    40801 gggtacgctt gacgacgatc aggcgttatc gctggttgaa gcgatggtcg aggccaacgg
    40861 cgagcgcgta atggcgctaa ttaatgaagc tgctgcccgt ggtatcgagt gggaagcgtt
    40921 gctggtggaa atgctcggtc tgttgcatcg tattgcgatg gtacaacttt cgcctgctgc
    40981 acttggcaac gacatggccg ccatcgagct gcggatgcgt gaactggcgc gcaccatacc
    41041 gccgacggat attcagcttt actatcagac gctgttgatt ggtcgcaaag aattaccgta
    41101 tgcgccggac cgccgtatgg gcgttgagat gacgctgctg cgcgcactgg cgttccatcc
    41161 gcgtatgcct ctgcctgatc cagaagtgcc acgccagtcc tttgcgcccg tcgcgccaac
    41221 ggcagtaatg acgccaacac aggtgccgcc gcaaccgcaa tcagcgccgc agcaggcacc
    41281 gactgtaccg ctcccggaaa ccaccagcca ggtgctggcg gcgcgccagc agttgcagcg
    41341 cgtgcaggga gcaaccaaag caaaaaagag tgaaccggca gccgctaccc gcgcgcggcc
    41401 ggtgaataac gctgcgctgg aaagactggc ttcggtcacc gatcgcgttc aggcgcgccc
    41461 ggtgccatcg gcgctggaaa aagcgccagc caaaaaagaa gcgtatcgct ggaaggcgac
    41521 cactccagtg atgcagcaaa aagaagtggt cgccacgccg aaggcgctga aaaaagcgct
    41581 ggaacatgaa aaaacgccgg aactggcggc gaagctggcg gcagaagcca ttgagcgcga
    41641 cccgtgggcg gctcaggtga gtcaactttc gctaccaaaa ctggtcgaac aggtggcgtt
    41701 aaatgcctgg aaagaggaga gcgacaacgc agtatgtctg catttgcgct cctctcagcg
    41761 gcatttgaac aaccgcggcg cacagcaaaa actggctgaa gcgttgagta cgttcaaagg
    41821 ttcaacggtt gaactgacta tcgttgaaga tgataatccc gcggtgcgta cgccgctgga
    41881 atggcgtcag gcgatatacg aagaaaaact tgcgcaggcg cgcgagtcca ttattgcgga
    41941 taataatatt cagaccctgc gtcgattctt cgatgcggag ctggatgaag aaagtatccg
    42001 ccccatttga tcgtaagcac agcttacgtt cgtcatcctt aacgtgattg agagagaaac
    42061 ctatgtttgg taaaggcggt ctgggtaacc tgatgaagca agcccagcag atgcaagaaa
    42121 aaatgcagaa aatgcaggaa gagatcgcgc agctggaagt caccggcgaa tctggcgcag
    42181 gtctggtaaa agtgaccatc aacggtgcac acaactgccg tcgcgtagag atcgacccga
    42241 gcctgctgga agacgacaaa gagatgctgg aagacctggt ggctgcagca ttcaacgacg
    42301 cagcacgtcg tattgaagaa acgcagaaag aaaaaatggc ctctgtatcc tccggaatgc
    42361 agctgccgcc tggctttaag atgccgttct gatgcaaacc agcccgctgt taacacagct
    42421 tatggaagca ctgcgctgtc tgccgggcgt tggcccgaag tcggcgcagc gtatggcgtt
    42481 cacgctgctt cagcgcgatc gtagcggcgg gatgcgtctg gcgcaggcgc tcacccgggc
    42541 gatgtcggaa atcggccact gcgccgattg tcgcaccttc accgaacagg aagtctgtaa
    42601 catctgttcg aatccacgtc gtcaggaaaa cggtcaaatc tgcgtggtgg agagtccggc
    42661 ggacatttac gccatcgagc agacggggca gttttcaggt cgttattttg tgttgatggg
    42721 acacctgtca ccgctggacg gcatcggtcc tgatgatatc gggcttgatc gtctggaaca
    42781 gcgtctggca gaggaaaaaa tcactgaagt gatcctcgcc accaacccca cggttgaagg
    42841 tgaagctacc gctaactaca ttgccgagct ttgtgcgcaa tatgacgtgg aagccagccg
    42901 aatcgctcat ggcgtaccgg ttggcggcga actggaaatg gtcgatggca ccacgctgtc
    42961 acactccctt gccgggcgtc ataagattcg tttttaagca aacgagagca ggatcacctg
    43021 ctctcgcttg aaattattct cccttgtccc catctctccc tcatcctgtt tttaacctta
    43081 aaatggcatt attgaggtag acctacatga aaggacaaga aactcgtggt tttcagtcag
    43141 aagtgaaaca gcttctgcac ctgatgatcc attctctcta ttccaataaa gaaatcttcc
    43201 tgcgtgagct tatctctaac gcctccgatg cggcggacaa gctgcgtttc cgcgcgcttt
    43261 ctaacccgga cctgtacgaa ggtgatggtg aactacgcgt tcgtgtctct ttcgataaag
    43321 acaagcgcac gctgaccatc tccgataacg gcgtggggat gacccgcgac gaagtgattg
    43381 accatctggg gactatcgct aaatccggca ccaaatcatt cctcgaatcc ctgggttctg
    43441 accaggcgaa agacagccag ctgatcggtc agtttggtgt tggtttctac tctgcgttta
    43501 tcgtggctga caaagtgacc gtgcgtactc gcgcggcagg cgaaaaacca gaaaacggcg
    43561 tcttctggga atcggctggc gaaggtgaat acaccgttgc cgacatcact aaagaagatc
    43621 gtggtactga aatcaccctg catctgcgtg aaggcgaaga cgagttcctc gatgactggc
    43681 gtgtgcgttc catcatcagc aaatattccg accatatcgc gctgccggta gagatcgaaa
    43741 aacgcgaaga gaaagacggc gaaaccatta tctcctggga gaaaatcaac aaagcgcagg
    43801 cgctgtggac tcgtaacaag tcggaaatca ccgatgaaga gtacaaagag ttctacaaac
    43861 acatcgccca cgactttaac gatccgctga cctggagcca caaccgcgtt gaaggtaagc
    43921 aggagtacac cagcctgctg tacattccgt ctcaggcacc gtgggatatg tggaaccgcg
    43981 atcataaaca cggtctgaaa ctgtatgttc agcgtgtgtt catcatggac gacgcagaac
    44041 agttcatgcc gaactatctg cgcttcgtgc gtggtctgat tgactccagc gatctgccgc
    44101 tgaacgtttc ccgtgaaatc ctccaggaca gcacggtaac gcgtaacctg cgcaatgcgc
    44161 tgaccaagcg tgtgctgcaa atgctggaaa aactggcgaa agacgacgcg gaaaaatacc
    44221 agaccttctg gcaacagttt ggcctggtac tgaaagaagg tccggcggaa gatttcgcta
    44281 accaggaagc gatcgccaaa ctgctgcgtt ttgcttctac ccataccgat tcttctgcgc
    44341 agaccgtgtc tctggaagac tacgtttccc gcatgaaaga agggcaggag aaaatctact
    44401 atatcaccgc agacagctac gcggcagcga agagcagccc gcacctggaa ctgctgcgta
    44461 agaaaggcat cgaagttctg ctgctttccg accgcatcga tgagtggatg atgaactatc
    44521 tgactgagtt cgacggtaaa ccgttccagt ctgtgtctaa agttgacgag tcgctggaaa
    44581 aactggctga cgaagttgat gagagcgcga aagaagcgga gaaagcactg actccgttca
    44641 tcgaccgtgt gaaagccctg ctcggcgagc gcgtgaaaga tgtccgtctg actcaccgtc
    44701 tgaccgatac gccagcgatc gtttcgaccg acgcggacga aatgagcact cagatggcga
    44761 aactgttcgc tgcggcgggc cagaaagtgc cagaagtgaa atacatcttc gaactgaacc
    44821 cggatcacgt actggtgaaa cgtgcggcgg atactgaaga tgaagcgaag ttcagcgagt
    44881 gggtagaact gctgctggat caggcgctgc tggcagaacg cggcacgctg gaagatccga
    44941 acctgtttat tcgtcgtatg aaccagctgc tggtttcctg atgtcatgcc ggatgacctt
    45001 cgtgtcatcc ggcatttttc ttttcttcat ctgcactttc cgcaaattat cttgccatta
    45061 accgtttcag cctcaggtgc ctttcttgag gcaatcgcct gttggtggta tcgtttatcg
    45121 ctttttcaaa aaattcgaca cattttaagg ggattttcgc aatgcgtatc attctgcttg
    45181 gcgctccggg cgcggggaaa gggactcagg ctcagttcat catggagaaa tatggtattc
    45241 cgcaaatctc cactggcgat atgctgcgtg ctgcggtcaa atctggctcc gagctgggta
    45301 aacaagcaaa agacattatg gatgctggca aactggtcac cgacgaactg gtgatcgcgc
    45361 tggttaaaga gcgcattgct caggaagact gccgcaacgg tttcctgttg gacggcttcc
    45421 cgcgtaccat tccgcaggca gacgcaatga aagaagcggg catcaatgtt gattacgttc
    45481 tggaattcga cgtaccggac gaactgattg ttgaccgtat cgtaggccgc cgcgttcacg
    45541 cgccgtctgg tcgtgtttat cacgttaaat tcaatccgcc gaaagtagaa ggcaaagacg
    45601 acgttaccgg tgaagagctg actacccgta aagacgatca ggaagagacc gtacgtaaac
    45661 gtctggttga ataccatcag atgactgcac cgctgatcgg ctactactcc aaagaagcgg
    45721 aagcgggtaa caccaaatac gcgaaagttg acggcaccaa gccggtggct gaagttcgcg
    45781 ctgatctgga aaaaatcctc ggctaatcaa gccgcagcag atctgctttc gggcaggtct
    45841 gctcatcctc ctcaggcaat acctcttaca gcaattagtt cttcttcttc acttttccgc
    45901 tacaattatc aacaagttga atcgataaga ggcggtaatg cgtcagacta aaaccggtat
    45961 cctgctggca aacctgggta cgcccgatgc ccccacacct gaagcggtaa aacgctatct
    46021 gaaacaattt ttaagcgaca gacgcgtggt tgatacctca cggttgttat ggtggccgtt
    46081 gctgcgcggc gtgattttgc cgctgcgttc gccgcgtgtg gcgaagctgt atgcctctgt
    46141 ctggatggaa ggtggctcgc cgctgatggt ttacagccgc cagcaacagc aggcgctggc
    46201 gcaacgttta ccggagacgc ccgtggcgct gggaatgagc tacggctcgc catcactgga
    46261 aagcgccgta gatgaacttc tggcagagca tgtagatcat attgtggtgc tgccgcttta
    46321 tccgcaatac tcctgttcaa cggtcggtgc ggtatgggat gaactggcac gcattctggc
    46381 gcgcaaacgt agcattccgg ggatatcgtt tattcgtgat tacgccgata accacgatta
    46441 tattaatgca ctggcgaaca gcgtacgtgc ttcttttgcc aaacatggcg aaccggatct
    46501 gctgttgctc tcttatcatg gcattcccca gcgttatgca gatgaaggcg atgattaccc
    46561 gcaacgttgc cgcacaacaa ctcgcgaact cgcttccgca ctggagatgg caccggaaaa
    46621 agtgatgatg acctttcagt cgcgctttgg ccgggaaccc tggctgatgc cttataccga
    46681 cgaaacgctg aaaatgctcg gagaaaaagg cgtgggtcat attcaggtga tgtgcccggg
    46741 ctttgctgcg gattgtctgg agacgctgga agagattgcc gagcaaaacc gtgaagtctt
    46801 cctcggcgct ggcgggaaaa aatacgaata tattccggcg cttaatgcta caccggagca
    46861 tatcgaaatg atggctaatc ttgttgccgc gtatcgctaa agctgagcgg taaagaactg
    46921 agcgccgtcg cgaagagcct cgtcggcggt tttcatcatc cgtgaataat gcaaaaaggc
    46981 gtgcagcgtg cctgggtaga gtttgaactc acagggctgc tgatgcgccg ctaacgtctg
    47041 gtaaagcaga tggctgtcat ccagcagcgg atcgaactcc gccccggcaa taaaacaggg
    47101 cggaacttcg cgagtgagat cattattaaa caggcagtaa tatggcgact cgcggtccgc
    47161 gtcgttgctt aaatacgcct cttcatacat ctgcaaatcc tgttgcgtta agccatccca
    47221 gacaccaccc aacagacgac gagccacgga atcccgtaat ccgtaaagcc cataccacag
    47281 caaaacgccc gcaactttac cgcaatcgat ctgtttatcg cgcaaccaca acgcactggc
    47341 gagcgccagc atggcacctg cggaatcacc ggcaaaacca atgcgggaca tattgatttg
    47401 ataatcctcc gcctgctggt ggaaataaca acaggcagcc acaatttcct ctatcgcttg
    47461 cggaaaacgc gcttcaggtg aaagggtgta atcaatgcca atcaccgtac attggctgta
    47521 gcttgccagc aggcgcatga tgcgatcgtg ggtatcgaga ttgccgagaa taaaaccgcc
    47581 tccatgcaaa taaaatagcg tcgccgggct atctggctgc ggataaaaga tacgtgtttt
    47641 cacctgccca tattttgttg gaaccctgta agctctggtt gccatttctg gagcgtccgc
    47701 attccagaat cggcgctcaa gcgtgtaata ctgtcgttgc tcagcaatcg ctcccgttgc
    47761 aggccagggc ggtaaatccg gctgaagagt attcacaacg gtcttcattt cagcagagat
    47821 aaggtccaga acgggtaatt tgttttccgg cttcatgcaa aaactccttt caaattacgt
    47881 cattgtaagg aaccactgcc atgaaaatgc gatcccgcct gctgatattg aaactggctg
    47941 cgtctcgcgc gctcccgtca gattgtgtta acattcgccg ctcagttaac cacccgtaaa
    48001 aacaaccatg aaatttcccg gtaaacgtaa atccaaacat tacttccccg taaacgcacg
    48061 cgatccgctg cttcagcaat tccagccaga aaacgaaacc agcgctgcct gggtagtggg
    48121 tatcgatcaa acgctggtcg atattgaagc gaaagtggat gatgaattca ttgagcgtta
    48181 tggattaagc gccgggcatt cactggtgat tgaggatgac gttgccgaag cgctttatca
    48241 ggaactaaaa cagaaaaacc tgattaccca tcagtttgcg ggtggcacca ttggtaacac
    48301 catgcacaac tactcggtgc tcgcggacga ccgttcggtg ctgctgggcg tcatgtgcag
    48361 caatattgaa attggcagct atgcctatcg ttacctgtgt aacacctcca gccgtaccga
    48421 tcttaactat ctacaaggcg tggatggccc gattggtcgt tgctttacgc tgattggcga
    48481 gtccggggaa cgtacctttg ctatcagccc gggccacatg aaccagctgc gggctgaaag
    48541 tattccggaa gatgtgattg ccggagcctc ggcactggtt ctcacttctt atctggtgcg
    48601 ttgcaagccg ggtgaaccca tgccggaagc aaccatgaaa gccattgagt acgcgaagaa
    48661 atataacgta ccggtggtgc tgacgctggg caccaagttt gtcattgccg agaatccgca
    48721 gtggtggcag caattcctca aagaccacgt ctctatcctt gcgatgaacg aagatgaagc
    48781 cgaagcgttg accggagaaa gcgatccgtt gttggcatct gacaaggcgc tggactgggt
    48841 agatctggtg ctgtgcaccg ccgggccaat cggcttgtat atggcgggct ttaccgaaga
    48901 cgaagcgaaa cgtaaaaccc agcatccgct gctgccgggg gctatagcgg aatttaatca
    48961 gtatgagttt agccgcgcca tgcgccacaa ggattgccag aatccgctgc gtgtctattc
    49021 gcacattgcg ccgtacatgg gcgggccgga aaaaatcatg aacactaatg gagcagggga
    49081 tggcgcactg gcagcgttgc tgcatgacat taccgccaac agctaccacc gtagcaacgt
    49141 accaaactcc agcaaacata aattcacctg gttgacgtat tcgtcattag cgcaggtgtg
    49201 taaatatgct aaccgtgtga gctatcaggt actgaaccag cattcacctc gtttaacgcg
    49261 cggcttgccg gagcgtgaag acagcctgga agagtcttac tgggatcgtt aagttatcgt
    49321 cggttcgtag gccagataag gcgttcacgc cgcatctggc attttgctct caatgcctga
    49381 tgcgacgctg gcgcgtctta tcaggcctac atatccgcgc ctattatccg gcaaccaccg
    49441 tttacctcgt caccacctca cccgccggtg gcgtttccag cagttccagc atagtacggg
    49501 caatttcacg ctcacccatc actacctgat tcgcaccacg ttcggtgata tacgccactt
    49561 catcgtcata atgggcgcgg gcaataatct caatatccgg atttttcgcg cgggcagatg
    49621 ccacaatctc gcccgcttca tagccgttgg gaatcgtcag gatcagccat tttgcacatt
    49681 ccagatgcgc cagttgcata atttcttcgt tagccgcatt gcccaatact gcgcggaccc
    49741 cgcgctcgcg cagctcatca acacgggttc gtgacgtctc aatcaccacc agcggaatat
    49801 cagaggcgag caatttctcc cccagcaggc tgcctacacg accgtaaccc accagtagcg
    49861 catggttgca aatatccact gggatctgct tctcttcttc gattgcctct tccagcgtct
    49921 gctcttccag cgtttcggtc ttcgccagat atttctccag tagtgcgaac agtaccgggt
    49981 tgagcataat cgacaggatc gcccctgcca gtaccaggtt ttgtccggcc tgcggcagta
    50041 aattcaatgc cattcctagt cccgccagga taaacgcgaa ctcaccaatc tgcgccaggc
    50101 tggcggcgat ggttaacgcc gtgcgctggg agtgaccaaa caaacgcaca aggaacaggg
    50161 ccgcgaccga tttaccaaac agaataattg ccagcgtcgc cagtactgcc agcggttgct
    50221 gaatcagaat taacggatca aacaacatcc cgacggagac aaaaaacagc accgcaaacg
    50281 cgtcgcgcaa tggcagtgta tcgtgggcgg cacggtgact cagttcagac tcgttcagta
    50341 ccatcccggc aaagaacgca ccgagtgcaa aggagacatc aaacagctct accgcaccaa
    50401 aggcaatccc taacgccagc gccagcaccg acagggtaaa cagctcgcgg gaaccggttg
    50461 ctgcgctgcg tgccataatc cacggcacca gacggcgacc taccagcatc ataatggcga
    50521 taaatgcgat caccttgccg atggtgatcc ccatatcgac tgcaagagtg gcaaagccca
    50581 catcgccctg ttccatcatt cctgctaccg cgggtagcaa taccagtgtc aggaccatca
    50641 ccaggtcttc cacaatcaac caaccgatgg cgatttgccc acgctgactg tcaattaatt
    50701 gccgttcttc aagtgcgcgc agtaacacca cggtactggc ggtggaaaga cataagccga
    50761 acacgatacc ggtcattaac gaccagccca gcacggcgga gagcgccata cccagcagcg
    50821 tcgccacggc tatctgggcg atcgcgccgg gaatggcgat ggactttacc gccatcaaat
    50881 ccttgagcga aaaatgcaaa ccgacgccga acatcagcaa aataacgccc agttcagcca
    50941 gttccggcgc aagcttagta tcggcaacaa agcccggagt gaatggtcct gccagcacac
    51001 ccgctaacag atatcccacc agaggagaaa tacgtagttt attggccagc atgccgagga
    51061 taaaggcgag cacaaggccg ccaacaatgg tggtgataag cggggtggcg tgatgcattc
    51121 cgtctccttt tcctggtggt tattgtccat ttttggccgg gaaaaccaaa attacaggta
    51181 atagtttatg acaatttcat tgatgatgtt tatgaataat tgttgaattt tgcagaaaaa
    51241 tggaattagc tgcaaaaaaa agcacggatc ggaaaacaga aggcggttta acagaggaaa
    51301 ggcttatggc gactgtaaaa cgatgcagcc aaagtttgcc tttggctgca atgaaatcag
    51361 tctttatgcc ggttatcagg caggaatatg gtcaacatcc ccaatagtgg caggaaagca
    51421 cagattttat agactaactc tatgctggtg tggtcggcga taagccccag aactgccgcc
    51481 cccagacctc ccatgccgaa agcaaaaccg aaaaatagtc cagaaaccat accgatacgt
    51541 cccggaagca gctcctgggc atagaccaga atggcagaga atgccgaagc gaggataaat
    51601 ccaataatca ccgttaaaac ccccgtccag tgcaggctgg cgtagggtaa aatcagcgta
    51661 aacggcgcaa cgccgaggat agagccccaa atcacatatt tccgtccaat tttatcccct
    51721 acaggcccgc caatcaccgt acctgccgca acggcaaaca ggaaggcaaa caaatgaagc
    51781 tgagcattct ggatagataa tccgaatttt tgcatcagat aaaaggtgta atagctgctg
    51841 atgctcgcca tatagaaata tttcgagaaa atgaggatta acagaatgct gaccgccaga
    51901 acaactttat tgcgcggcag tggattgata atcgtcgctt tgggttttcc tttattcatt
    51961 cggtgctgtg ccgagtacca acggctgatt tgcgccaaca ccacgatcgc cagcagtgcc
    52021 gcaagcacaa accaggcaac gttgcctttg ccataaggcg cgataatcac cgccgccagc
    52081 aaggggccca gggaactgcc aaagttgccg ccgacctgaa atatagattg cgccaggcca
    52141 tgacgcccgc cggaagccat acgggccacg cgagaagatt ccggatgaaa gaccgatgaa
    52201 ccggtaccga ccagcgccgc tgccagcaga actgcgccga aactgcccgc cagcgccagc
    52261 agcaccagac cacttaaggt aaagcacatg ccaatcggca acgaccacgg catcggatat
    52321 ttatcggtcc agtagccgac caccggttgc agtagcgaag aggcgagctg gaaggtgagg
    52381 gttatcatgc caatctgcat aaatgtcaga gaaaattctg actgaagcag tggataaatc
    52441 gccagaatca gcgattggat catgtcgttc agcagatgtg agaggctgat agcacctaaa
    52501 ataccaaacg atgttcgggc cttagtcgtt gacgcagccg cgcccgccac aggctgggtt
    52561 tgttcactca ttgccatagg aaagtcactt tttcagggtt gcgatgtaaa gaatgatctt
    52621 atttgtgatt attacccgac taacatacct gtatgcgccg cctgaaggaa gtctcaacac
    52681 ggaatacaga atttctaatc tggatgcaga tttatcttca ccggacgcag acttgtttat
    52741 gatgtcgcgt catactattt ttcaacacgt tgaaatcagg tcagggagag aagtatgaaa
    52801 ttattgcagc ggggcgtggc gttagcactg ttaaccacat ttacactggc gagtgaaact
    52861 gctctggcgt acgagcagga taaaacctac aaaattacag ttctgcacac caatgatcat
    52921 catgggcatt tttggcgcaa tgaatatggt gaatatggtc tggcggcgca aaaaacgctg
    52981 gtggatggta tccgcaaaga ggttgcggct gaaggcggta gcgtgctgct actttccggt
    53041 ggcgacatta acactggcgt gcccgagtcc gacttacagg atgccgaacc tgattttcgc
    53101 ggtatgaatc tggtgggcta tgacgcgatg gcgatcggta atcatgaatt tgataacccg
    53161 ctcaccgtat tacgccagca ggaaaagtgg gctaagttcc cgttgctttc cgccaatatc
    53221 taccagaaaa gtaccggcga gcgcctgttt aaaccatggg cgctgtttaa gcgtcaggat
    53281 ctgaaaattg ccgttattgg cctgacgacg gatgacacag ccaaaattgg taatccggaa
    53341 tatttcactg atatcgaatt tcgtaagccc gctgatgaag cgaagctggt gattcaggag
    53401 ctgcaacaga cagaaaagcc agacattatt atcgcggcga cccatatggg gcattacgac
    53461 aatggtgagc acggctctaa cgcaccgggc gatgtggaga tggcacgcgc gctgcctgcc
    53521 ggatcgctgg cgatgatcgt cggtggtcac tcgcaagatc cggtctgtat ggcggcagaa
    53581 aacaaaaaac aggtcgatta cgtgccgggt acgccatgca aaccggatca acaaaacggc
    53641 atctggattg tgcaggcgca tgagtggggc aaatacgtgg gacgggctga ttttgagttt
    53701 cgtaatggcg aaatgaaaat ggttaactac cagctgattc cggtgaacct gaagaagaaa
    53761 gtgacctggg aagacgggaa aagcgagcgc gtgctgtaca cccctgaaat cgctgaaaac
    53821 cagcaaatga tctcgctgtt atcaccgttc cagaacaaag gcaaagcgca gctggaagtg
    53881 aaaataggcg aaaccaatgg tcgtctggaa ggcgatcgtg acaaagtgcg ttttgtacag
    53941 accaatatgg ggcggttgat tctggcagca caaatggatc gcactggtgc cgactttgcg
    54001 gtgatgagcg gaggcggaat tcgtgattct atcgaagcag gtgatatcag ctataaaaac
    54061 gtgctgaaag tgcagccatt cggcaatgtg gtggtgtatg ccgacatgac cggtaaagag
    54121 gtgattgatt acctgacagc cgtcgcacaa atgaagccag attcaggtgc ctacccgcaa
    54181 tttgccaacg ttagctttgt ggcgaaagac ggcaaactga acgacctgaa aatcaaaggc
    54241 gaaccggtcg atccggcgaa aacttaccgt atggcgacat taaacttcaa tgccaccggc
    54301 ggtgatggct atccgcgcct tgataacaaa ccgggctatg tgaataccgg ctttattgat
    54361 gccgaagtgc tgaaagcgta tatccagaaa agctcgccgc tggatgtgag tgtttatgaa
    54421 ccgaaaggtg aggtgagctg gcagtaatga accccgtcgc ggcgtcatga tatgacgccg
    54481 ccatttatga atattgaaat cttgcatcgt aatcgcgatg tgtcaaacca aatgccatca
    54541 gcatataatt ctgcggatca ccaggctgtt cttcatctgc ttcaaaccaa agtgtcacgc
    54601 ctgagcgatt gacgtggatt aagagtgtat ggggcgattg acggcggatt tgcagatcag
    54661 cgccttcgtt aacaagcatg ttaaatacac gcgtaacgtt caactcagcg acgttattca
    54721 atattccttt atcaaatcgg atcgctttgc ccaaatggat ttggctcaga taatcgcacc
    54781 aaatcatata tagcttgttg ttttcgaaat gaaactcaac attaccaaat agccagatat
    54841 aaaaaccatt tcccatatca tttttagagt ccggctcaac aaagttttgt tccaaccatg
    54901 ccattgtttg ccccagttcg acaaaatcga attttccgtt aaagacaaaa ttgtggaagc
    54961 taacatcaac aagatggtca aatttcatct ttcgctcctt tgtgaatttt ttacatgctt
    55021 caaaagccag cgtaatttac atcggtgcag gcgactctcc agtgttgagc gaaaatttgc
    55081 gatgtcgaca ttggaccgat ctaccgcagc aacccccgtg tattgtggga tcggtgaagg
    55141 gattttttgt aactttccca gcgccagcag ttgttgaatg atcctgctac cgtcgtggta
    55201 atcatcgtaa atagccactg gcagaggctg ccaggcgggg gtcgatttga taaatccgga
    55261 aaaaaaatac gggtccagat cgccgaaatc aaagccgtag gcgaggggaa cttcaatatt
    55321 ttgctctgtt tttttattct caaatgtaca atgaaaaccg tgaaaacaat actgccactt
    55381 tcccattttg ccttttcgct tacggttgca tttgaagggg gcaaaggtaa ctagcgggtt
    55441 attttccgcc atctcaacgt tcagttgttc agccaatgca aatataagct ctgtcgccag
    55501 cttacgatat tctttcgcgc agtgttcgaa aaaatcctga tggcggttaa tctcttcaag
    55561 cgatattaat ctggcgatcc tgataaccgg gtcggcagaa tgcatgccaa caacaaattc
    55621 atcgagctgt gctttcagca ccagcccgaa ttcctcacga tacctctctg cttcgctggc
    55681 aaaatgcaga tgtaatacct tctctctcga tgtttgccga agtaaggcga tatccggtat
    55741 ctcatctgca caagcgatac ttaatgtctc tgtaatcctt tccacatctc atcttccgtg
    55801 tgcatcactt aatcgcgacg ggcgatatca gcaaatttgg catcgagtat ctttgccagg
    55861 tcgcttgccg ccagttcgat atccagtccg cgcttgccgc cggaaacata aatagtggca
    55921 aattcttgtg cgggggcgtc gataatcgtt ggcagacgtt ttttctgccc cagtgggcta
    55981 atccccccta ccagataccc cgtcgaacgc tgcgcgacca tcggatcggc catctcaact
    56041 ttcttggcac ccagcgcttt tgctactttt ttaagatcca gttgacctgc gaccggcgta
    56101 acagccacgg caaggtgttt catatcaccg ttcactgcga ccagcagcgt tttgtagacc
    56161 tgatccggat tcaaacctaa ttttttgacg acttcatcgc caaaattggt ttcagcagga
    56221 tcgtgctcgt aggtgtggat ttgaaacgaa atcttgtttt tttcgagtaa tttaactgcg
    56281 ggtgtcatgg taattttcct gccttaaaca aaaaaacgaa gcaagcatac gcctgatggc
    56341 ggtccaaaaa aaagagtcat cttgcctaag agtattggca ggatggtgag attgagcgac
    56401 aatcgagtta caccgtcgct taaagtgacg gcataataat aaaaaaatga aattcctctt
    56461 tgacgggcca atagcgatat tggccatttt tttagcgcaa catctgcggc aaattccctt
    56521 ctccatacag gtgtagtgca ccgaccgcga ccacatagcg ccccggcggc atggcgcgta
    56581 atttgtcccg ccaggcgagg tttcgctgat gcatcagcac atcgtacagc gactgactga
    56641 acgtattggg cagcgttata tcattatttt gcggcggtgc attcagccac cagctcatca
    56701 tttgttgcag caaccgtgcg ttggtgtgcc agtgggtcag cgtatcgtcc agcagcgcca
    56761 gtcctttgtc agggagctgg agcaacatgg caatctggtt ttcagcccct tccagttcaa
    56821 tcacgggttt atgttgttgc ttagccgcct gcaatagctg gtaatcgata ccgtattctg
    56881 cccgcagccc cagtttttgc gcctgcgtcg cctgaagaac catcgcgatt tgccacagcg
    56941 gttgggtaga aaagagcgac ggagaaatgc ccatctcctg gctaatgtgc tgcaggtttt
    57001 ggagttggtc ctcgctaatg cgctcttcca gggcctcgca ggcaggcaaa ttggcaaaag
    57061 gcgtatcgct ggtggaaaca tccgcctcga cgatcagcgc atcggcgttt ttgagctttt
    57121 tgagcaaacg ggtgggcagg ggagccatat cgtggctacc catatgaata ctgccgatca
    57181 gatgaaaatg gcgattgccg ggaagagaga tatcgatagc aggccaggtg taatgattac
    57241 cgcgcagcgc ggcccaaagc gtttttaccc ggtacaacag atccatacga cctccctttg
    57301 tgaaatatca tgctagcgcg cggggaggga tggcgcaaat ggagttgatt gttgcacttg
    57361 tgcacagtgc ttgtgtgcat tacaatgatt aaataatgga caaggagatg agagaggaat
    57421 ggctcaaaag aaaaatataa ggagttttcg tgatgcctgg ttggctgatt tttttgtcca
    57481 ttcaacgcca catcgaaaga tccctgctga aatccatacc acgttatcac gaaagttgga
    57541 catcattaat gctgccactt ctcatcggga tttacgctcc cctccgggga accgatacga
    57601 agagttatcc gggaaattac aggaatactc ctccattaga gtgaataaac agtaccgact
    57661 gatttttaaa tgggttaatg gcaaggccga agaattgttt ttagacccac acaactacta
    57721 aaacatagct gtcgtattga cgagaaaggt aaaactatga aacaggcaac aagaaaaccg
    57781 acgacacccg gagatattct tctctatgaa tatctggaac cgctcgattt gaaaatcaat
    57841 gagttagcag agttgctgca tgttcatcgt aatagcgtca gtgcactgat caataacaat
    57901 cgtaaactca ccactgagat ggcattccgt ctggcgaaag tttttgatac cacagtcgat
    57961 ttttggctaa acctccaggc ggcagttgat ctttgggaag ttgaaaacaa catgcgtacc
    58021 caggaagaat tgggacggat tgaaacagtg gctgaatatt tggcacgccg tgaagagcgt
    58081 gcaaaaaagg tcgcgtagaa taatttccct ataacggatg cgccacccta aagcagcgca
    58141 tccgcaatga tgtacttatt ccttcggttt aaaccgcagc aatcggttgg cgttactcac
    58201 tacggtgatc gacgagagcg ccattgccgc tccggcaacc accgggttaa gcagtgttcc
    58261 agtgaacggc cacaaaatac cggcggcgac cggaatacca atgctgttgt agataaacgc
    58321 accgagcaga ttctgcttca tgttgcgcag cgttgcgcgg gaaattgcaa gcgcatccgc
    58381 aacacccatc aggctatggc gcatcagggt aatcgccgcg gtttcaatgg caacatcact
    58441 gccgccaccc atcgcaatac cgacatccgc ctgagccagc gctggcgcgt cgttaatgcc
    58501 gtcgcctacc attgccacct gacgtccttc actttgcaga cgtttgatcg cttcggcttt
    58561 accgtctggc agcacgccgg ctatcacctc atcaatccct gcttctttgg cgatcgcatt
    58621 ggcggtggtc gggttatccc cggttagcat caccaggcga tatcccgctt tatgcaggcg
    58681 ttgcagcgcc gccacgctat cactacgcaa cggatcgcgt acagccagca gggctaccgc
    58741 tttcccgtca accgccagca gcacaggcgt tgccccttgc gatgcctgag ctgaaatatc
    58801 tgcttcaatt gctttggtat cgacctgttg gtcatttaac agcgcctgat tgcccagcaa
    58861 taacgcatga ccttcagctt caccgctcac gcccagcccg cgcaatgtgc ggaaaccgtt
    58921 gacctgcggt agctgcatat cacctgcttt atcgaggatc gcccgtgcca gagggtggct
    58981 ggaaccttgc tccagcgccg ccgccagacg caatgcttgc gcttcatcaa tatcagcaaa
    59041 tgtttttact gcgacaacct gcggcttccc ttcggtcagc gtcccggttt tatcgaacac
    59101 tacggtgtcg agcgtactgg cgcgttgcag cgcgtcggcg tcccgtacca gcacgccaaa
    59161 ctcagccgcc cgcccgacgc ccgaaataat cgacatcggc gtcgccagcc ccagcgcaca
    59221 cggacaggca ataatcagca ccgtggtggc aatcaccagg gtatagacaa tctgcggtgc
    59281 cggaccaaag aaataccaga ttgccgcact gatcagcgca atcaccacca ctaccggcac
    59341 aaatacggct gagattttat ccgccagctg accgatttct ggcttactgc tctgggcctg
    59401 gcgcaccata cgaataatcc gcgacagcgt agtatggctg ccaaccgcac tggcgcgaaa
    59461 cagcacgcta ccgtcctgca ccactgtccc ggcatggacg ctatcgcctt cgcctttttg
    59521 ctgcgggata ggttcgcccg tcagcatcgc ttcatccagc catgcttcgc cctgggtaat
    59581 ctctccatct accggtacgc gatcgccggt cgtcaggcgc agcaacatac ctggctgcac
    59641 ttccgccagt ggcacgcttt tttcaccttc gtcagtaacc aggcgcgccg tcggcggagt
    59701 taaatcgagc aatttctcca gcgcctttga agaacgctga cgtgcgcgcg cttccagcat
    59761 atgaccgaga ttaatcagac cgataatcat cgcactggct tcatagtaaa gatgccgcgc
    59821 ttccatcggg aaccactgcg gccacaggtt gacgctcatc gaatagagcc acgccacacc
    59881 cgttcccagc gccaccagcg tatccatcgt cgccgcaccg ttcagcaggc ttttccatgc
    59941 gctgcggtaa aaatgcccgc cggcgaaaac cattaccgcc agggttatca ggccgataac
    60001 taaccacagg ctgcggttgt cagcggtgac catcatgtta tcgccgatca tcccccagac
    60061 catcaccggg atacccaccg ccagcgcgac aattgcctgc cagcggaagc gcttcatcgt
    60121 agcgacggcg gtttcttgct ggcgctcgcg gcgtttagcg tcatcttcaa tcgcttccgc
    60181 gccgtagccc gctttttcca ccgcctgcac taaatcttgt ggggaggcac tgcccatcac
    60241 cagcgcagta cgctccgcca ggtttacccg tgcctgagtg acgcccggta cgctttgcaa
    60301 cgcattttgt acgcgggtga cacagctggc gcagctcatg ccgctcagca gcaactgctg
    60361 gctgtcatcg tcatcggcgg tcgctgccgg aagcgcctca gaaaccgctg tcagtgcttc
    60421 cgacgggatt gatgactccg ccagcggttt agcctttggg tggcttacag atgcgtcata
    60481 acccgcttgt ttgatggttt cgattagctg ttctgcactg gcagtcccgg taacgtgcgc
    60541 ttcagtgata gacacatccg cctgttcaac atccgggcgc tgttcgagac tttctttcac
    60601 gcgtttaacg cagtgaccgc aggacaggcc gtccagggtc aggtcgatag tttgtgacat
    60661 aaaacactcc tttaagacag ttttgactgg ctgtgataaa ggttaaacct tccagcaagg
    60721 ggaaggtcaa gaaattaata aaccaggcgg gtaaaagtcc gtaaagatta aaaaatcggc
    60781 tcgatttgca tcaggattag acatttatct ctttgttttc ctgtagttaa gttgcgggtg
    60841 ctaagttaaa tcaggatgca tgaaaatcgg caccggggtg aggaattacc tcccgcatct
    60901 ataaaaagga gttaacaaaa gatgttagat gcaaacaaat tacagcaggc agtggatcag
    60961 gcttacaccc aatttcactc acttaacggc ggacaaaatg ccgattacat tccctttctg
    61021 gcgaatgtcc ctggtcaact ggcggcagtg gctatcgtga ccagcgatgg caacgtctat
    61081 agtgcgggtg acagtgatta ccgctttgcg ctggaatcca tctcgaaagt ctgtacgtta
    61141 gcccttgcgt tagaagatgt cggcccgcag gcggtacagg acaaagttgg cgctgacccg
    61201 accggattgc cctttaactc agttatcgcc ttagagttgc atggcggcaa accgctttcg
    61261 ccactggtaa atgctggcgc tattgccacc accagcctga ttaacgctga aaatactgaa
    61321 caacgctggc agcgaatttt acatatccaa caacaactgg ctggtgagca ggtagcgctc
    61381 tctgatgaag tcaaccagtc ggaacaaaca accaacttcc ataaccgggc cattgcctgg
    61441 ctgctgtact cagccggata tctctattgt gatgcaatgg aagcctgtga cgtgtacacc
    61501 cgtcagtgct ccacgctcat caatactatt gaactggcaa cgcttggcgc gacgctggcg
    61561 gcgggtggtg tgaatccgct gacgcataaa cgcgttcttc aggccgacaa tgtgccgtac
    61621 attctggccg aaatgatgat ggaagggctg tatggtcgct ccggtgactg ggcgtatcgc
    61681 gttggtttac cgggcaaaag cggtgtaggt ggcggtattc tggcggtcgt tcctggcgtg
    61741 atgggaattg ccgcgttttc accaccactg gacgaagaag gcaacagtgt tcgcggtcaa
    61801 aaaatggtgg catcggtcgc taagcaactc ggctataacg tgtttaaggg ctgatcatga
    61861 tgaacacgga aggtaataac ggtaacaaac ccctcggtct atggaacgtc gtttccatcg
    61921 gcattggggc aatggtgggg gcggggatct tcgcgctgct ggggcaggct gcgttgctaa
    61981 tggaagcctc gacctgggtc gcctttgctt ttggcggtat tgtggcgatg ttttccggtt
    62041 atgcctatgc gcgtctgggg gcgagctatc ccagcaatgg cggtattatc gacttctttc
    62101 gtcgcggatt aggcaacggc gttttttcgc tggcgctctc gttattgtac ctgttgacgc
    62161 tggcggtgag tatcgccatg gtcgcccgtg cttttggcgc ttatgccgtg cagtttttgc
    62221 atgaaggcag tcaggaggag caccttattt tgctctacgc attggggatc attgcggtga
    62281 tgacgctttt caactcctta agcaaccatg cggtagggcg gctggaagtg atcctcgtcg
    62341 gcattaaaat gatgatcctg ttattgctga ttattgccgg tgtctggtcg ctgcaaccgg
    62401 cgcatatttc cgtttctgcg ccccccagct ccggggcgtt cttctcctgt atcgggataa
    62461 ccttccttgc ctatgcgggc tttggcatga tggcgaacgc ggcggataaa gtgaaagatc
    62521 cggaggtcat aatgccacgg gcgtttctgg tggcgattgg cgttaccacg ttgctttata
    62581 tctcgctggc gctggttttg cttagcgatg tatcggcatt agagttagaa aaatatgccg
    62641 ataccgccgt agcgcaggct gcttctccgc tcctcgggca tgtggggtat gtgatcgtcg
    62701 tcatcggcgc tttactggcg acggcttcag ccatcaacgc aaacctgttc gccgtgttta
    62761 acatcatgga caacatgggt agcgaacgcg aactgccgaa gctaatgaat aaacctctgt
    62821 ggcggcagag tacctggggt aacatcatcg tcgtggtgtt gattatgctg atgacggcgg
    62881 cgctgaattt aggctcactc gccagcgttg ccagtgccac ctttttgatc tgctatctgg
    62941 cggtgtttgt ggtggcgatc cgcctgcgtc atgatattca cgcctcgttg ccgattctta
    63001 tcgttggtac gttggttatg ttgttggtga tcgttggctt tatctacagt ctctggtccc
    63061 agggcagccg tgcgctgata tggattattg gcgcactctt actcagcctt attgtggcaa
    63121 tggtcatgaa gcgcaataaa accgtataac atctctctgt gcgcagtacc tcctgtatta
    63181 ttgtggtggc ggtcgatatt cgcactggca aaaaaacgtg cttgaatatc tgttgaaacc
    63241 ctttaacaaa gcacaggagg cgttgcgcga acgatgaaca ttagcgatgt agcaaaaatt
    63301 accggcctga ccagcaaagc cattcgcttc tatgaagaga aggggctggt gacgccgccg
    63361 atgcgcagcg aaaacggtta tcgcacctac acgcagcagc atctcaacga actgacctta
    63421 ctgcgccagg cacgccaggt gggctttaac ctggaagaga gcggcgaact ggtgaatctg
    63481 tttaacgatc cgcagcgtca cagcgccgac gtcaaacgtc gtacgctgga gaaagtagcg
    63541 gagatcgaac agcatatcga agaactgcaa tccatgcgta accagctgct ggcgctggcg
    63601 aatgcctgcc ccggtgatga cagcgccgac tgcccgatta tcgaaaatct ctccggctgc
    63661 tgtcatcatc gggcggggtg attaaggctt gcgaaaatag tcgtccatta ttttcgttag
    63721 ctccggacgt tccccgtcat cattgatggc aaaatccaga aaaaaagcat ctgcttgcgg
    63781 cgttcggttt tgcgccatca accggaaggt gagggggcct gaccaggttt cggatgggct
    63841 gatgtgctta tcagggccga aactctccca tgcgctgggc ggtaatgttt tgcacgcggt
    63901 gagttttggt atgtggtatt gcagctctgt tttgtgcaga cattccccgc caggaaatac
    63961 cagggtttgt tgctcatcat taaaatgggt tgccgtgctg agcaccagtg ttttgagcgg
    64021 tcgcccttcc agcgcggcgg cagtagcctt atggtgtccg tcgagtagaa cgcagagata
    64081 ggaatcaggg agataccagg ccagaccacg ggcaattcca gttttcgaga gcagacaaaa
    64141 accgggcggc tccctgttca tcctccgcaa aaccgctgta gacaaattcc gcacaggtag
    64201 ggcatccagc ctcggaccag aaacgtatta acgggacatc ctgccagcag agcgaatgga
    64261 taagaccgtg cgagtgcgga tcgtaaatca cacattgacg cagaaatcct ttcccggact
    64321 tcaccgaaaa aatgacgccc gcagcggtat ttttcagcag acgtacttca ggttttcgaa
    64381 agggggaaac atggctactc cttagccgaa cattcaatta acactatgtg gggggctata
    64441 tgccggatgc ggcgtaaacg ccttatccgg catgggtttt ggtacagatt tccaggtgtg
    64501 ataagacgcg tcaggcgatg accgcacgga taatcagcgt tattccttct atcgcaatca
    64561 cctcaacatg cgtaccagcg ccgagatcct cgctggcgct gacaggccat gaactgtcac
    64621 cgacgcgcat atgaccgcgc ccgttgacca gcggagattc cagaacaaaa cgtcggccaa
    64681 tcaactgctg cccgcgctgg tttaaatgac tgtcgctgtg cttttgttcg cgcacccgcc
    64741 gcgacaacca tttccaccac agccaggcgg cgagcagcgt caggacggca aacattaccc
    64801 cttgccactc ccaacccagc ggcagcaacc agaccaccag accggtaatc accgctgcca
    64861 cgccgctcca caacagataa ccattgccgc ccaacatctc ggctgccagc agcaaaccgc
    64921 cgagacttag ccagaaaata tgtggatgaa cgaccattaa ctccatcatg gctgagtccg
    64981 tttgttggcg ctgtctttca ccagctcagc aatcccggca atcgacccca tcaggctgct
    65041 ggcctctaat ggcatcatca ccactttgct gttactggac gaaccgatct gctgtaacgc
    65101 ttcggtgtat ttctgtgcga cgaagtagtt caccgcctga atatcaccgg aggcgatggc
    65161 ttcagacacc attttggtgg cgcgggcttc tgcttcagcg gaacgttcac gcgcttcagc
    65221 ctgtaaaaac gccgactgac gttcgccttc cgctttcagg atttgtgact gtttttcacc
    65281 ttcggctttg aggatttccg cctgacggat cccttccgct tcaaggatgt aagcgcgttt
    65341 ggtacgttcc gctttcatct gcgcgttcat tgaagagata agctctgcgg gcgggcgcac
    65401 atcacgaatt tcgatacggg tgactttaat cccccacggg ttggtggcct catcgacaat
    65461 atgcagcaga cgcgagttaa tgctgtcgcg ctgagagagc atttcatcaa gttccattga
    65521 acccagcacg gtacggatgt tagtcatggt caggttgatg atcgccagct ccagattgct
    65581 gacttcataa gccgcgcgcg gcgcgtcaat cacctgaata aagcacacag cgtcgatggt
    65641 aacgttggcg ttatctttcg agataacttc ctgggaaggg atgtcgagca cttgctccat
    65701 catattgatc ttgcgaccaa tgcgatccat aaacggcacc accaggctta gacctggctg
    65761 taacgttttg gtatagcgac caaagcgttc taccgtccat tgatagccct gcggcacgat
    65821 tttgacgccc gcgccgacaa tgaccagcgc gacaaaaatg agaatcggga taaagataag
    65881 catcggaaaa acctcctgtt gtaccgtcca taatcagcaa aattgctgct tgattaaaca
    65941 aattatacct gattactgaa agagagttcc cccttattcc tgcgaaggat aaactgtttt
    66001 tagtaaaaat cagaaaaagg gaacagcgat gcaggaaaat agtcctttgc ttcagctaca
    66061 aaacgtagga tatctggcgg gtgatacgaa gattcttaat aacatcaatt tttcgctgcg
    66121 tgctggcgaa tttaagttaa ttaccggtcc ttctggttgt ggcaaaagta cactgctaaa
    66181 aatagttgct tcattgatca gcccaaccag cggaacgtta ctgtttgaag gtgaggatgt
    66241 cagcacacta aagccagaaa tctaccgtca gcaagtctct tactgcgccc agacgccgac
    66301 gctgtttggc gatacggtat acgataacct gatttttccc tggcagatcc gtaaccagca
    66361 gcctgacccg gccatttttc tcgattttct tgaacgcttc gctttaccgg acagcatttt
    66421 gacgaagaat atcgccgagc tatctggtgg tgaaaaacaa cgcatctcat tgattcgtaa
    66481 cctgcaattt atgccgaagg ttttattgct cgacgaaatc accagtgcgc tggatgaaag
    66541 taataaacat aacgttaatg agatgatcca ccgttatgta cgcgagcaaa atattgctgt
    66601 gctgtgggtg acacacgata aagacgaaat taatcatgcg gataaagtga ttacactgca
    66661 accgcatgct ggagaaatgc aggaagcacg ctatgaactc gcataatatt actaacgaat
    66721 cattagcact ggcattaatg ctggtggtgg tggcaatctt aattagccat aaagaaaaac
    66781 tggcgctgga gaaagatatt ctctggagcg tcgggcgcgc gataatccag ctgattattg
    66841 tcggctatgt gctgaagtat attttcagcg tggatgatgc cagcctgaca ttattgatgg
    66901 tgttatttat ctgctttaat gcggcgtgga acgcgcaaaa acgcagtaaa tatattgcta
    66961 aagcttttat ctcatcgttt attgccatta cggtcggggc gggaattacc ctggcggtac
    67021 tgatcctctc tgggtcgatt gaatttatcc cgatgcaggt gatccctatc gccgggatga
    67081 tagccggtaa cgccatggta gcggtggggt tgtgttacaa caatttaggg caacgggtca
    67141 ttagcgaaca gcaacagatt caggagaaac tgagtcttgg cgcgacgccg aaacaggctt
    67201 cagcgatatt gattcgcgac agtattcgcg cggctttaat tccgacggtc gattcagcaa
    67261 aaacggtagg cttagtgagt ttaccaggaa tgatgtccgg gctgatattt gccgggattg
    67321 atccggtgaa ggcgattaaa tatcagatta tggtgacctt tatgctgctc tcaaccgcca
    67381 gcttgtcgac cattattgcc tgctatttaa cctatcgtaa gttttacaat tcgcgccacc
    67441 aattggtggt gacgcaattg aagaagaaat gatgcgatgc cggatgcgtc ttatcagacc
    67501 tacatggcac ctgcaatgta ttgaattagc acggtttggt aggccggata aggcgttcac
    67561 gccgcatccg gcggctatgc gcggatgcct gatgcgacgc tggcgcgtct tatcaggcct
    67621 acaaaccagc acagcatcgc atttcaacgg aaaatcaata caacaacgca tacagctggc
    67681 ggcgatactt cgacgccagt gcatcacccg tacccagcgc agcgaggatc tcctggaatg
    67741 ttttacgtgt ctgaccatct gcggcggtga gatctttacg cagatgccca aacagtaact
    67801 ccagcgcctc ttcattgcgc ccaacctggt gcagttgcag tgccagttgc gtcgccagtg
    67861 cggcatcttc tggattctcc gccacctgct gttgcaactg ttgaatttcc ggcgtatcag
    67921 ccgcctgctt cagcagttcg atttgcgcca ccagcccctg atagcgggtg tcctgatcct
    67981 gcaacggaat ggttttcagc acggcttctg catcttcaga acggttcagc gcaatcagcg
    68041 tttctgccaa cagcaggccg atttccccgt tctgattcga caactgccag gcgtctttca
    68101 gcaatggcag ggcgtcggtg taattgcctt cctgcatcag ttgcatcgcc tgctgcgctt
    68161 tcagctcttc ttcacgcggc agcactttat ccagcagggc gcggatcgcc tcttccggtt
    68221 gcggcccctg gaagccgtct actggttgcc cgttctggaa cagatatacg gtcggaatcg
    68281 cgcgcagacc aaactgggcg gcaatcatct gctccgcgtc gcagtccagc ttcgccagaa
    68341 taaattgccc gttgtactgc gccgcgaggc tttccagaat tggggttaac tgcaaacagt
    68401 gctggctacg ttcagaccaa aaatagaaca gcaccggagt ggtcatcgac tgttcaagaa
    68461 cctgttgcag gttagattcg ttaatgttga caatattttc tacggacatg gagtcgctct
    68521 ctgttgtcga tattttcttt gacatggggg cttaagcgcg cgcttcaact cacccctgca
    68581 atattttgtc catcacgcgc cccggcagca ggcgcttaag caccattacc gccctggtca
    68641 ccagcgtcac cggatagcgc atcttcggct tctcgctaat aaaagcatgg cgcactttgc
    68701 cgaccaccgc ttccggtccc aacgtaaagc gggcggcgat gccaggattt tcgactggtt
    68761 tatcactttg cgtctggttg acgttgtcgg tgaagcgagt acgaatggga ccaggttcga
    68821 tcaggctgac tttaattccg ctgtggcgca gctccatgcg cagtgcgtct gaccacgcct
    68881 ccagcgcata tttactggcc gcgtaagcgc cacgacccgg tgtggagatt aatcccatca
    68941 ccgatgaggt catcacaata cgcccttcac ggtgcggcaa catcgcgggt aacaggcgca
    69001 tggtgagctg gtgtgcgccg aaaaagttag cggaaaactg ctgttccatc tgcgcacggc
    69061 tgatggtgga aagggggcca tacatgccga atccggcatt gttaaagatc ccatacagac
    69121 aattatcggt cagggcgatc acttcgtcgg ctgcgcgatc aacactttct ggcgaatcca
    69181 gatcgatcaa cacgccggta aatcccatgc tgttcatgcg ctcaacatca tccggtttcc
    69241 ggcaacctgc cagcacatga aaaccctggc gttttaattc gagtgcgctt tccaggccaa
    69301 taccactgga acatccggta attaagaccg atttttgcat aactttacct gtcaggatct
    69361 ccgttgcttt atgagtcatg atttactaaa ggctgcaact gcttcgccat ccagtcggca
    69421 ataaacggct gggcgtcgcg gttgggatga ataccgtcat cctgcatcca ttgtggcttg
    69481 aggtagacct cttccataaa aaagggcagc agcggaacat caaactcttt ggcgagtttg
    69541 gggtaaatgg cgctaaaggc ttcattataa cggcgaccat agtttgcagg cagacgtatt
    69601 tgcattaaca atggttcagc gttggcggct ttgacatcct gcaaaatctg gcgcagcgtt
    69661 tgctcggttt gctgtggctg aaaaccacgc aaaccgtcat tgccgcccag ttcaaccagc
    69721 acccagcgcg gctgatgctg tttcagcaga gccggaaggc gcgccagccc ttgttgcgag
    69781 gtgtcaccgc tgatgctggc attgactacc gacgttttac tctgccactt atcattcaac
    69841 aaggcaggcc aggccgcgct ggcagacatt cgatacccgg cgctcaggct atcacccaga
    69901 atcaataacg tgtccgctgc ggcggcacgg aaggttaaca ggaccaggaa caggaagggc
    69961 aaatgccagc ggaaaacatt gttgaagttc atcatcttaa gaagtccgtc ggtcaggggg
    70021 agcatgaact ctccatcctc accggagttg agctggttgt caaacgtggc gagaccatcg
    70081 cactggtggg cgagtcggga tcgggtaagt caaccttgct ggcgatcctc gccgggctgg
    70141 atgacggcag cagtggcgaa gtgagtctgg tgggacaacc gctacataat atggacgaag
    70201 aagcgcgggc aaagttgcgc gcgaagcacg taggctttgt ttttcagtca tttatgttaa
    70261 ttcctaccct taacgcgctg gaaaacgtcg agcttccggc gctgctgcgc ggtgagagta
    70321 gcgcagaaag tcgtaacggg gcgaaagcgt tgctcgaaca gttagggctg ggtaaacgtc
    70381 ttgatcatct tccggcacag ctttctggcg gcgaacagca acgagtggcg ctggcgcgag
    70441 cctttaatgg tcgacctgat gtgctgtttg ccgacgaacc caccggtaac cttgaccgcc
    70501 agacgggcga taaaattgcc gatctgcttt tttccctcaa ccgtgagcat ggcaccacgt
    70561 tgattatggt gacccacgac ctgcaactgg cggcacgctg tgaccgctgc ttacggctgg
    70621 tgaacgggca gttgcaggag gaagcatgat tgcacgttgg ttctggcgcg aatggcgctc
    70681 gccgtcgcta ttaattgtct ggctggcgct tagcctggcg gtggcctgcg tgctggcgct
    70741 gggcaatatc agcgatcgca tggagaaggg cttaagccag caaagccgtg agtttatggc
    70801 gggcgatcgg gcgttgcgca gttcgcgcga agtgccgcaa gcatggctgg aggaagcgca
    70861 aaagcgcggc ctgaaagttg gcaagcagct gactttcgcc accatgacct ttgcaggcga
    70921 cacgccgcaa ctggcgaacg tcaaagcggt ggatgatatc tacccgatgt atggcgatct
    70981 gcaaactaat ccccctggcc tgaaaccgca ggcgggcagc gtattgctgg ccccacgcct
    71041 gatggcactg cttaacctga aaacgggcga taccattgac gtgggcgatg ccaccttgcg
    71101 gattgccgga gaagtgattc aggaaccgga ttccggtttt aatcctttcc agatagcccc
    71161 acgcctgatg atgaatctgg cggatgttga taaaaccgga gccgtgcagc cggggagtcg
    71221 ggtcacctgg cgttataaat tcggcggcag cgagaaccag ctcgacggct atgagaaatg
    71281 gttgttaccc cagcttaaac ccgaacaacg ctggtacggt ctggaacagg acgaaggcgc
    71341 gctggggcga tcgatagaac gttcgcaaca gttcctgctg ctttcggcgc ttctgacctt
    71401 gctgctggcg gtggcggcgg tcgcggtggc gatgaatcat tactgtcgca gtcgctacga
    71461 tctggtggcg atcctcaaga cgctgggggc agggcgagcg caactgcgta agttaatcgt
    71521 cggtcagtgg ttgatggtgc tggtactttc agccgttacc ggtggggcga taggcctgtt
    71581 gttcgaaaac gtgttgatgg tgctgctcaa gcccgttctg cctgctgcac taccgccagc
    71641 cagcctctgg ccgtggctgt gggcgctcgg caccatgacg gttatttcgc tgctggtggg
    71701 gctacgacca taccgcctgt tgttggcaac gcagccttta cgcgtattac gtaatgatgt
    71761 ggtagcgaac gtctggccgc tgaagtttta tctgccgatt gtcaccgtgg tggttgtgct
    71821 actgctcgcc gggctgatgg gcggcagtat gctgctctgg gcggtgctgg cgggcgcggt
    71881 agtactggct ttgctgtgcg gtgtcctggg ctggatgctg ctgaatgtac ttcgccgcat
    71941 gacgctgaaa tcgctacctc tgcgcctggc ggttagccgc ctgttacgtc agccgtgggc
    72001 gacgttgagc cagctttcgg cattttcgct ctcctttatg ctgctggcac tgttgctggt
    72061 gttgcgtggc gatctgctcg accgctggca acagcagcta ccgccagaaa gtcctaacta
    72121 ctttttaatc aacatcgcta cggaacaggt tacgccgctg aaagcgtttc tcgcggaaca
    72181 tcagatagtc ccggaatcgt tttatccggt ggtgcgggcg cggttgacgg cgattaacga
    72241 caagccgaca gaaggcaatg gagatgaggc gctcaaccgc gagctgaatc tcacctggca
    72301 aaatacgcgg cctgatcata acccgatcgt cgccggtaac tggccgccaa aagccggtga
    72361 agtgtcgatg gaagaggggc tggcgaaacg cttaaacgtt gccctcggcg ataccgtgac
    72421 ttttatgggc gatacccagg agttccgcgc taaagtgacc agcctgcgca aagtggactg
    72481 ggaaagtctg cggcctaatt tctattttat tttcccggaa ggggcattag acgggcaacc
    72541 gcagagctgg cttaccagtt tccgctggga gaatggcaac ggcatgttga cgcaactcaa
    72601 ccgccagttc ccgaccatta gcctgttaga cattggcgcg attttaaaac aggtcggtca
    72661 ggtgctggag caggtaagtc gggcgctgga agtgatggtg gtgctggtca ccgcctgcgg
    72721 tatgttgctg ttgctggcac aggtgcaggt gggaatgcgt cagcgtcatc aggagctggt
    72781 ggtgtggcgc actctcggtg cgggcaaaaa actgctgcgc acgacattgt ggtgtgagtt
    72841 cgccatgctc gggtttgtat ccggcctggt ggccgcaatt ggtgcggaaa cggcactggc
    72901 ggtactgcaa tcgaaagtgt ttgatttccc gtgggagcca gactggcgat tgtggattgt
    72961 gctgccgtgc agcggtgcgc tactgctgtc gctttgcggc ggctggctgg gtgcgcgact
    73021 gcttaagggt aaggcgctat tcaggcagtt tgtggggtga tgtgataaag ccgtttattt
    73081 ctacctggta gataaacggc tttattatta gtgcatcacg taattaaaaa aatgttatgc
//
LOCUS       DAHHTG010000006        71455 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.006, whole genome shotgun sequence.
ACCESSION   DAHHTG010000006 DAHHTG010000000
VERSION     DAHHTG010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 71455)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 71455)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..71455
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.006"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            48..1448
                     /gene="sthA"
                     /locus_tag="NBI74_000424"
     CDS             48..1448
                     /gene="sthA"
                     /locus_tag="NBI74_000424"
                     /EC_number="1.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Si-specific NAD(P)(+) transhydrogenase"
                     /protein_id="HCD1930800.1"
                     /translation="MPHSYDYDAIVIGSGPGGEGAAMGLVKQGARVAVIERYQNVGGG
                     CTHWGTIPSKALRHAVSRIIEFNQNPLYSDHSRLLRSSFADILNHADNVINQQTRMRQ
                     GFYERNHCEILQGNARFVDEHTLALDCPDGSVETLTAEKFVIACGSRPYHPTDVDFTH
                     PRIYDSDSILSMHHEPRHVLIYGAGVIGCEYASIFRGMDVKVDLINTRDRLLAFLDQE
                     MSDSLSYHFWNSGVVIRHNEEYEKIEGCDDGVIMHLKSGKKLKADCLLYANGRTGNTD
                     SLALQNIGLETDSRGQLKVNSMYQTAQPHVYAVGDVIGYPSLASAAYDQGRIAAQALV
                     KGEATAHLIEDIPTGIYTIPEISSVGKTEQQLTAMKVPYEVGRAQFKHLARAQIVGMN
                     VGTLKILFHRETKEILGIHCFGERAAEIIHIGQAIMEQKGGGNTIEYFVNTTFNYPTM
                     AEAYRVAALNGLNRLF"
     gene            complement(1431..2348)
                     /gene="oxyR"
                     /locus_tag="NBI74_000425"
     CDS             complement(1431..2348)
                     /gene="oxyR"
                     /locus_tag="NBI74_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator OxyR"
                     /protein_id="HCD1930801.1"
                     /translation="MNIRDLEYLVALAEHRHFRRAADSCHVSQPTLSGQIRKLEDELG
                     VMLLERTSRKVLFTQAGMLLVDQARTVLREVKVLKEMASQQGETMSGPLHIGLIPTVG
                     PYLLPHIIPMLHQTFPKLEMYLHEAQTHQLLAQLDSGKLDCVILALVKESEAFIEVPL
                     FDEPMLLAIYEDHPWANRECVPMADLAGEKLLMLEDGHCLRDQAMGFCFEAGADEDTH
                     FRATSLETLRNMVAAGSGITLLPALAVPPERKRDGVVYLPCIKPEPRRTIGLVYRPGS
                     PLRSRYEQLAEAIRARMDGHFDKVLKQAV"
     gene            complement(2615..3988)
                     /gene="argH"
                     /locus_tag="NBI74_000426"
     CDS             complement(2615..3988)
                     /gene="argH"
                     /locus_tag="NBI74_000426"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HCD1930802.1"
                     /translation="MALWGGRFTQAADQRFKQFNDSLRFDYRLAEQDIVGSVAWSKAL
                     VTVGVLTAEEQAQLEEALNVLLEDVRARPQQILESDAEDIHSWVEGKLIDKVGQLGKK
                     LHTGRSRNDQVATDLKLWCKDTVSELLTANRQLQSALVETAQNNQDAVMPGYTHLQRA
                     QPVTFAHWCLAYVEMLARDESRLQDALKRLDVSPLGCGALAGTAYEIDREQLAGWLGF
                     ASATRNSLDSVSDRDHVLELLSAAAIGMVHLSRFAEDLIFFNTGEAGFVELSDRVTSG
                     SSLMPQKKNPDALELIRGKCGRVQGALTGMMMTLKGLPLAYNKDMQEDKEGLFDALDT
                     WLDCLHMAALVLDGIQVKRPRCQEAAQQGYSNATELADYLVAKGVPFREAHHIVGEAV
                     VEAIRQGKPLEDLPLSELQKFSLVIGEDVYPILSLQSCLDKRAAKGGVSPQQVAQAIA
                     FAQARLG"
     gene            complement(4049..4825)
                     /gene="argB"
                     /locus_tag="NBI74_000427"
     CDS             complement(4049..4825)
                     /gene="argB"
                     /locus_tag="NBI74_000427"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418394.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HCD1930803.1"
                     /translation="MMNPLIIKLGGVLLDSEEALERLFSALVNYRESHQRPLVIVHGG
                     GCVVDELMKGLNLPVKKKNGLRVTPADQIDIITGALAGTANKTLLAWAKKHQIAAVGL
                     FLGDGDSIKVTQLDEELGHVGLAQPGSPKLINSLLENGYLPVVSSIGVTDEGQLMNVN
                     ADQAATALAATLGADLILLSDVSGILDGKGQRIAEMTAAKAEQLIEQGIITDGMIVKV
                     NAALDAARTLGRPVDIASWRHAEQLPALFNGMPMGTRILA"
     gene            complement(4833..5837)
                     /gene="argC"
                     /locus_tag="NBI74_000428"
     CDS             complement(4833..5837)
                     /gene="argC"
                     /locus_tag="NBI74_000428"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HCD1930804.1"
                     /translation="MLNTLIVGASGYAGAELVTYVNRHPHMNITALTVSAQSNDAGKL
                     ISDLHPQLKGIVDLPLQPMSDIGEFSPGVDVVFLATAHEVSHDLAPQFLEAGCVVFDL
                     SGAFRVNDVAFYEKYYGFTHQYPELLEQAAYGLAEWCGNKLKEANLIAVPGCYPTAAQ
                     LALKPLIDADLLDLNQWPVINATSGVSGAGRKAAISNSFCEVSLQPYGVFTHRHQPEI
                     ATHLGADVIFTPHLGNFPRGILETITCRLKPGVSQVQVAQALQQAYAHKPLVRLYDKG
                     VPALKNVVGLPFCDIGFAVQGEHLIIVATEDNLLKGAAAQAVQCANIRFGYAETQSLI
                     "
     gene            5991..7142
                     /gene="argE"
                     /locus_tag="NBI74_000429"
     CDS             5991..7142
                     /gene="argE"
                     /locus_tag="NBI74_000429"
                     /EC_number="3.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine deacetylase"
                     /protein_id="HCD1930805.1"
                     /translation="MKNKLPPFIEIYRALIATPSISATEEALDQSNADLITLLADWFK
                     DLGFNVEVQSVPGTRNKFNMLASCGQGAGGLLLAGHTDTVPFDDGRWTRDPFTLTEHD
                     GKLYGLGTADMKGFFAFILDALRDVDVTKLAKPLYILATADEETSMAGARYFAETTAL
                     RPDCAIIGEPTSLQPVRAHKGHISNAIRIMGQSGHSSDPSRGVNAIELMHDAIGHILQ
                     LRDNLKERYHYEAFTVPYPTLNLGHIHGGDASNRICACCELHMDIRPLPGMTLNELNG
                     LLNDALAPVSERWPGRLTVDELHPPIPGYECPPNHKLVEVVEKLLGAKTEVVNYCTEA
                     PFIQTLCPTLVLGPGSINQAHQPDEYLETRFIKPTRELITQVIHHFCWH"
     gene            7444..10095
                     /gene="ppc"
                     /locus_tag="NBI74_000430"
     CDS             7444..10095
                     /gene="ppc"
                     /locus_tag="NBI74_000430"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004203687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HCD1930806.1"
                     /translation="MNEQYSALRSNVSMLGKVLGETIKDALGEHILERVETIRKLSKS
                     SRAGNDANRQELLTTLQNLSNDELLPVARAFSQFLNLANTAEQYHSISPKGEAASNPE
                     VIARTLRKLKNQPELSEDTIKKAVESLSLELVLTAHPTEITRRTLIHKMVEVNACLKQ
                     LDNKDIADYEHNQLMRRLRQLIAQSWHTDEIRKLRPSPVDEAKWGFAVVENSLWQGVP
                     NYLRELNEQLEENLGYKLPVEFVPVRFTSWMGGDRDGNPNVTADITRHVLLLSRWKAT
                     DLFLKDIQVLVSELSMVEATPELLALVGEEGAAEPYRYLMKNLRSRLMATQAWLEARL
                     KGEELPKPEGLLTQNEELWEPLYACYQSLQACGMGIIANGDLLDTLRRVKCFGVPLVR
                     IDIRQESTRHTEALGELTRYLGIGDYESWSEADKQAFLIRELNSKRPLLPRNWQPSAE
                     TREVLDTCQVIAEAPQGSIAAYVISMAKTPSDVLAVHLLLKEAGIGFAMPVAPLFETL
                     DDLNNANDVMTQLLNIDWYRGLIQGKQMVMIGYSDSAKDAGVMAASWAQYQAQDALIK
                     TCEKAGIELTLFHGRGGSIGRGGAPAHAALLSQPPGSLKGGLRVTEQGEMIRFKYGLP
                     EITVSSLSLYTGAILEANLLPPPEPKESWRRIMDELSVISCDLYRGYVRENKDFVPYF
                     RSATPEQELGKLPLGSRPAKRRPTGGVESLRAIPWIFAWTQNRLMLPAWLGAGTALQK
                     VVEDGKQSELEAMCRDWPFFSTRLGMLEMVFAKADLWLAEYYDQRLVDKALWPLGKEL
                     RNLQEEDIKVVLAIANDSHLMADLPWIAESIQLRNIYTDPLNVLQAELLHRSRQAEKE
                     GHEPDPRVEQALMVTIAGIAAGMRNTG"
     gene            10278..12011
                     /locus_tag="NBI74_000431"
     CDS             10278..12011
                     /locus_tag="NBI74_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase CptA"
                     /protein_id="HCD1930807.1"
                     /translation="MHSTEVQAKPLFSWKALGWALLYFWFFSTLLQAIIYISGYSGTN
                     GIRDSLLFSSLWLIPVFLFPKRIKIIAAVIGVVLWAASLAALCYYVIYGQEFSQSVLF
                     VMFETNTNEASEYLSQYFSLKIVLIALAYTAVAVLLWTRLRPVYIPKPWRYVVSFALL
                     YGLILHPIAMNTFIKNKPFEKTLDNLASRMEPAAPWQFLTGYYQYRQQLNSLTKLLNE
                     NNALPPLANFKDESGNEPRTLVLVIGESTQRGRMSLYGYPRETTPELDALHKTDPNLT
                     VFNNVVTSRPYTIEILQQALTFANEKNPDLYLTQPSLMNMMKQAGYKTFWITNQQTMT
                     ARNTMLTVFSRQTDKQYYMNQQRTQSAREYDTNVLKPFQEVLKDPAPKKLIIVHLLGT
                     HIKYKYRYPEDQGKFDGNTEHVPPGLNAEELESYNDYDNANLYNDHVVASLIKDFKAT
                     DPNGFLVYFSDHGEEVYDTPPHKTQGRNEDNPTRHMYTIPFLLWTSEKWQATHPRDFS
                     QDVDRKYSLAELIHTWSDLAGLSYDGYDPTRSVVNPQFKETTRWIGNPYKKNALIDYD
                     TLPYGDQVGNQ"
     gene            12237..13088
                     /locus_tag="NBI74_000432"
     CDS             12237..13088
                     /locus_tag="NBI74_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCD1930808.1"
                     /translation="MYHDVSYLLSRLINGPLSLRQIYFASSNGPVPDLAYQVDFPRLE
                     IVLEGEFIDTGAGAALVPGDVLYVPAGGWNFPQWQAPATTFSVLFGKQQLGFSVVQWD
                     GKQYQNLAKQHVARRGPRIGSFLLQTLNEMQMQSQEQQTARLIVASLLSHCRDLLGSQ
                     IQTASRSQALFEAIRDYIDERYASALTRESVAQAFYISPNYLSHLFQKTGAIGFNEYL
                     NHTRLEHAKTLLKGYDLKVKEVAHACGFVDSNYFCRLFRKNTERSPSEYRRQYHSQLT
                     EKPTTPE"
     gene            complement(13075..13416)
                     /locus_tag="NBI74_000433"
     CDS             complement(13075..13416)
                     /locus_tag="NBI74_000433"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HCD1930809.1"
                     /translation="MAYLVAVTACVSGVAHTYMAAERLEKLCQLEKWGVSIETQGALG
                     TENRLADEDIRRADVALLITDIELAGAERFEHCRYVQCSIYAFLREPQRVMSAVRKVL
                     SAPQQTHLILE"
     gene            complement(13418..14296)
                     /locus_tag="NBI74_000434"
     CDS             complement(13418..14296)
                     /locus_tag="NBI74_000434"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418387.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[formate-C-acetyltransferase]-activating enzyme"
                     /protein_id="HCD1930810.1"
                     /translation="MTSSAGQRISCNVVETRRDDVARIFNIQRYSLNDGEGIRTVVFF
                     KGCPHLCPWCANPESISGKIQTVRREAKCLHCAKCLRDADECPSGAFERIGRDISLDA
                     LEREVMKDDIFFRTSGGGVTLSGGEVLMQAEFATRFLQRLRLWGVSCAIETAGDAPAS
                     KLLPLAKLCDEVLFDLKIMDAAQARDVVKMNLQRVLENLRLLVSEGVNVIPRLPLIPG
                     FTLSRENMQQALDVLIPLNIKQIHLLPFHQYGEPKYRLLGKTWSMKEVAAPSSADVAT
                     MREMAERAGFQVTVGG"
     gene            complement(14262..16559)
                     /locus_tag="NBI74_000435"
     CDS             complement(14262..16559)
                     /locus_tag="NBI74_000435"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HCD1930811.1"
                     /translation="MTNRISRLKTALFANTREISLERALLYTASHRQTEGEPVIMRRA
                     KATAYILEHVEISIRDEELIAGNRTVKPRAGIMSPEMDPYWLLKELDQFPTRPQDRFA
                     ISEEDKRIYREELFPYWEKRSMKDFINGQMTDEVKAATSTQIFSINQTDKGQGHIIID
                     YPRLLNHGLGELVAQMQQHCQQQPENHFYQAALLLLEASQKHILRYAELAETMAASCT
                     DGPRREELLTIAEISRHNAEHKPQTFWQACQLFWYMNIILQYESNASSLSLGRFDQYM
                     LPFYQTSLTQGEDPAFLKELLESLWVKCNDIVLLRSTSSARYFAGFPTGYTALLGGLT
                     ENGRSAVNVLSFLCLDAYQSVQLPQPNLGVRTNALIDTPFLMKTAETIRLGTGIPQIF
                     NDEVVVPAFLNRGVLLEDARDYSVVGCVELSIPGRTYGLHDIAMFNLLKVMEICLHEN
                     EGNAALTYEGLLEQIRAKISHYITLMVEGSNICDIGHRDWAPVPLLSSFISDCLEKGR
                     DITDGGARYNFSGVQGIGIANLSDSLHALKGMIFDQQRLSFDELLSVLKANFATPEGE
                     KVRARLINRFEKYGNDIDEVDNISAELLRHYCKEVEKYQNPRGGYFTPGSYTVSAHVP
                     LGAVVGATPDGRFAGEQLADGGLSPMLGQDAQGPTAVLKSVSKLDNTLLSNGTLLNVK
                     FTPATLEGEAGLRKLADFLRAFTQLKLQHIQFNVVNADTLREAQQRPQDYAGLVVRVA
                     GYSAFFVELSKEIQDDIIRRTAHQL"
     gene            complement(16610..16930)
                     /locus_tag="NBI74_000436"
     CDS             complement(16610..16930)
                     /locus_tag="NBI74_000436"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HCD1930812.1"
                     /translation="MTKIIAVTACPSGVAHTYMAAEALESAAKAKGWEVKVETQGSIG
                     LENELTAEDVASADMVILTKDIGIKFEERFAGKTIVRVNISDAVKRADAIMSKIEAHL
                     AQTA"
     gene            complement(16945..18024)
                     /locus_tag="NBI74_000437"
     CDS             complement(16945..18024)
                     /locus_tag="NBI74_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit EIIC"
                     /protein_id="HCD1930813.1"
                     /translation="MNELVQILKNTRQHLMTGVSHMIPFVVSGGILLAVSVMLYGKGA
                     VPDAVADPNLKKLFDIGVAGLTLMVPFLAAYIGYSIAERSALAPCAIGAWVGNSFGAG
                     FFGALIAGIIGGIVVHYLKKIPVHKVLRSVMPIFIIPIVGTLITAGVMMWGLGEPVGA
                     LTNSLTQWLQGMQQGSIVMLAVIMGLMLAFDMGGPVNKVAYAFMLICVAQGVYTVVAI
                     AAVGICVPPLGMGLATLIGRKNFSAEERETGKAALVMGCVGVTEGAIPFAAADPLRVI
                     PSIMVGSVCGAVTAALVGAQCYAGWGGLIVLPVVEGKLGYIAAVAVGAVVTAVCVNVL
                     KSLARKNGSSTEEKEDDLDLDFEIN"
     gene            18333..20834
                     /gene="ptsP"
                     /locus_tag="NBI74_000438"
     CDS             18333..20834
                     /gene="ptsP"
                     /locus_tag="NBI74_000438"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HCD1930814.1"
                     /translation="MALIVEFICELPNGVHARPASHVETLCNTFSSQIEWHNLRTDRK
                     GNAKSALALIGTDTLVGDNCQLLISGADEQEAHQRLSQWLRDEFPHCDAPLAEVKSDE
                     LEPLPVSLTNLNPQIIRARTVCSGSAGGILTPISSLDLNALGNLPAAKGVDAEQSALE
                     NGLTLVLKNIEFRLLDSDGATSAILEAHRSLAGDTSLREHLLAGVSAGLSCAEAIVAS
                     ANHFCEEFARSSSSYLQERALDVRDVCFQLLQQIYGEQRFPAPGKLTQPAICMADELT
                     PSQFLELDKNHLKGLLLKSGGTTSHTVILARSFNIPTLVGVDIDALTPWQHQTIYIDG
                     NAGAIVVEPGESVARYYQQEARVQDALREQQRVWLTQQARTADGIRIEIAANIAHSVE
                     AQAAFGNGAEGVGLFRTEMLYMDRTSAPGESELYNIFCQALESANGRSIIVRTMDIGG
                     DKPVDYLNIPAEANPFLGYRAVRIYEEYASLFTTQLRSILRASAHGSLKIMIPMISSM
                     EEILWVKEKLAEAKQQLRNEHIPFDEKIQLGIMLEVPSVMFIIDQCCEEIDFFSIGSN
                     DLTQYLLAVDRDNAKVTRHYNSLNPAFLRALDYAVQAVHRQGKWIGLCGELGAKGSVL
                     PLLVGLGLDELSMSAPSIPAAKARMAQLDSRECRQLLNQAMACRTSLEVEHLLAQFRM
                     TQQDAPLVTAECITLESDWRSKEEVLKGMTDNLLLAGRCRYPRKLEADLWAREAVFST
                     GLGFSFAIPHSKSEHIEQSTISVARLQAPVRWGDDEAQFIIMLTLNKHAAGDQHMRIF
                     SRLARRIMHEEFRNALVNAASADAIASLLQHELEL"
     gene            20846..21508
                     /gene="fsa"
                     /locus_tag="NBI74_000439"
     CDS             20846..21508
                     /gene="fsa"
                     /locus_tag="NBI74_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HCD1930815.1"
                     /translation="MELYLDTANVAEVERLARIFPIAGVTTNPSIIAASKESIWEVLP
                     RLQKAIGDEGILFAQTMSRDAQGMVKEAKRLRDAIPGIVVKIPVTSEGLAAIKMLKKE
                     GITTLGTAVYSAAQGLLAALAGAKYVAPYVNRVDAQGGDGIRTVQELQALLEMHAPES
                     MVLAASFKTPRQALDCLLAGCESITLPLDVAQQMLNTPAVESAIEKFEHDWNAAFGTT
                     HL"
     gene            21519..22622
                     /gene="gldA"
                     /locus_tag="NBI74_000440"
     CDS             21519..22622
                     /gene="gldA"
                     /locus_tag="NBI74_000440"
                     /EC_number="1.1.1.6"
                     /EC_number="1.1.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418380.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional L-1,2-propanediol
                     dehydrogenase/glycerol dehydrogenase"
                     /protein_id="HCD1930816.1"
                     /translation="MDRIIQSPGKYIQGADVINRLGEYLKPLAERWLVVGDKFVLGFA
                     QSTVEKSFKDAGLVVEIAPFGGECSQNEIDRLRGIAETAQCGAILGIGGGKTLDTAKA
                     LAHFMGVPVAIAPTIASTDAPCSALSVIYTDEGEFDRYLLLPNNPNMVIVDTKIVAGA
                     PARLLAAGIGDALATWFEARACSRSGATTMAGGKCTQAALALAELCYNTLLEEGEKAM
                     LAAEQHVVTPALERVIEANTYLSGVGFESGGLAAAHAVHNGLTAIPDAHHYYHGEKVA
                     FGTLTQLVLENAPVEEIETVAALSHAVGLPITLAQLDIKEDVPAKMRIVAEAACAEGE
                     TIHNMPGGATPDQVYAALLVADQYGQRFLQEWE"
     gene            22898..23515
                     /locus_tag="NBI74_000441"
     CDS             22898..23515
                     /locus_tag="NBI74_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1287 domain-containing protein"
                     /protein_id="HCD1930817.1"
                     /translation="MKASLALFSLLTVFTSYSLKSPAVPPTVVQIQANTNLAIADGAR
                     QQIGSTLFYDPAYVQLTYPGGDVPQERGVCSDVVIRALRSQKVDLQKLVHEDMAKNFA
                     AYPQKWQLKRPDSNIDHRRVPNLETWFTRHDKTRPTSKNPSDYQAGDIVSWRLDNGLA
                     HIGVVSDGFARDGTPLVIHNIGAGAQEEDVLFSWRMVGHYRYFAK"
     gene            complement(23542..24447)
                     /gene="yijE"
                     /locus_tag="NBI74_000442"
     CDS             complement(23542..24447)
                     /gene="yijE"
                     /locus_tag="NBI74_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418378.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine transporter YijE"
                     /protein_id="HCD1930818.1"
                     /translation="MSAAGKSNPLAISGLVVLTLIWSYSWIFMKQVTSYIGAFDFTAL
                     RCIFGALVLFIVLLLRGRGMRPTPFKYTLAIALLQTCGMVGLAQWALVSGGAGKVAIL
                     SYTMPFWVVIFAALFLGERLRRGQYFAILIAAFGLFLVLQPWQLDFSSMKSAMLAILS
                     GVSWGASAIVAKRLYARHPRVDLLSLTSWQMLYAALVMSVVALLVPQREIDWQPTVFW
                     ALAYSAILATALAWSLWLFVLKNLPASIASLSTLAVPVCGVLFSWWLLGENPGAVEGS
                     GIVLIVLALALVSRKKKEAVSVKRI"
     gene            complement(24541..26721)
                     /gene="katG"
                     /locus_tag="NBI74_000443"
     CDS             complement(24541..26721)
                     /gene="katG"
                     /locus_tag="NBI74_000443"
                     /EC_number="1.11.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase/peroxidase HPI"
                     /protein_id="HCD1930819.1"
                     /translation="MSTSDDIHNTTATGKCPFHQGGHDQSAGGGTTTRDWWPNQLRVD
                     LLNQHSNRSNPLGEDFDYRKEFSKLDYYGLKKDLKALLTESQPWWPADWGSYAGLFIR
                     MAWHGAGTYRSIDGRGGAGRGQQRFAPLNSWPDNVSLDKARRLLWPIKQKYGQKISWA
                     DLFILAGNVALENSGFRTFGFGAGREDVWEPDLDVNWGDEKAWLTHRHPEALAKAPLG
                     ATEMGLIYVNPEGPDHSGEPLSAAAAIRATFGNMGMNDEETVALIAGGHTLGKTHGAG
                     PTSNVGPDPEAAPIEEQGLGWASTYGSGVGADAITSGLEVVWTQTPTQWSNYFFENLF
                     KYEWVQTRSPAGAIQFEAVDAPEIIPDPFDPSKKRKPTMLVTDLTLRFDPEFEKISRR
                     FLNDPQAFNEAFARAWFKLTHRDMGPKSRYIGPEVPKEDLIWQDPLPQPIYNPTEQDI
                     IDLKFAIADSGLSVSELVSVAWASASTFRGGDKRGGANGARLALMPQRDWDVNAAAVR
                     ALPVLEKIQKESGKASLADIIVLAGVVGVEKAASAAGLSIHVPFAPGRVDARQDQTDI
                     EMFELLEPIADGFRNYRARLDVSTTESLLIDKAQQLTLTAPEMTALVGGMRVLGANFD
                     GSKNGVFTDRVGVLSNDFFVNLLDMRYEWKATDESKELFEGRDRETGEVKYTASRADL
                     VFGSNSVLRAVAEVYASSDAHEKFVKDFVAAWVKVMNLDRFDLL"
     gene            complement(27050..27940)
                     /gene="metF"
                     /locus_tag="NBI74_000444"
     CDS             complement(27050..27940)
                     /gene="metF"
                     /locus_tag="NBI74_000444"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HCD1930820.1"
                     /translation="MSFFHASQRDALNQSLAEVQGQINVSFEFFPPRTSEMEQTLWNS
                     IDRLSSLKPKFVSVTYGANSGERDRTHSIIKGIKDRTGLEAAPHLTCIDATPAELRII
                     ARDYWNNGIRHIVALRGDLPPGSGKPEMYASDLVTLLKEVADFDISVAAYPEVHPEAK
                     SAQADLLNLRRKVDAGANRAITQFFFDVESYLRFRDRCVSAGIDVEIIPGILPVSNFK
                     QAKKFADMTNVRIPAWMAQMFDGLDDDAETRKLVGANIAMDMVKILSREGVKDFHFYT
                     LNRAEMSYAICHTLGVRPGL"
     gene            complement(28322..29875)
                     /locus_tag="NBI74_000445"
     CDS             complement(28322..29875)
                     /locus_tag="NBI74_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HCD1930821.1"
                     /translation="MKIKILAAGIALTLPFWACAKDVTIIYTNDLHAHVEPYKVPWIA
                     DGKRDIGGWANITTLVKQEKAKNKATWFFDAGDYFTGPYISSLTKGKAIIDIMNTMPF
                     DAVTIGNHEFDHGWDNTLLQLSQAKFPIVQGNIFYQNSSKSFWDKPYTIIEKDGVKIG
                     VIGLHGVFAFNDTVSAATRVGIEARDEIKWLQRYIDELKGKVDLTVALIHEGVPARQS
                     SMGGTDVRRALDKDIQTASQVKGLDILITGHAHVGTPEPIKVGNTLILSTDSGGIDVG
                     KLVLDYKEKPHNFTVKNFELKTIYADEWKPDPQTKQVIDGWNKKLDEVVQQTVAQSPV
                     ELKRAYGESASLGNLAADALLVAAGKNTQLALTNSGGIRNEIPAGAITMGGVISTFPF
                     PNELVTMELMGKQLRSLMEHGASLSNGVLQVSKGLEMKYDSNRPVGQRVITLTLNGKP
                     IEDATVYHIATQSFLADGGDGFTAFTEGKARNTTGGYYVYHAVVDYFKAGNTITDEQL
                     NGMRVKDIK"
     gene            30008..31138
                     /locus_tag="NBI74_000446"
     CDS             30008..31138
                     /locus_tag="NBI74_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016242804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytoplasmic protein"
                     /protein_id="HCD1930822.1"
                     /translation="MSTLFTINACKSFGCRNLGQPTSTDYSWPDYRLGYPALHCRACG
                     SYPPLFDEQQFRDWLTVHLSTFATEKGHFCPVCYGTETICYGHNPQGSQRIQCRNCKK
                     VWTPKQYQKEITTPEIIETVALLVPFQGVSSGQKLYVLISFDALRGNILHLSTNYTQH
                     QAGESLHYRYRGNAEPELHDNNIVQRVDMREAQFLRRSQFDEIQYGSAALKRNAKGAI
                     LRPVITAHGHFRVLNILFPTVKTHVISHECFLRGAIITAWADLFRQQQGEIWFIEEEI
                     ADDTDNMPWRFQGKTYHGWWKNQWQLWVQGKNRKMVCALTGGKSSKAEMLSLATSRHF
                     IDWLHKQAVFTHSAPLSAGRVTQILLSLAQDYNNCASRLISD"
     gene            complement(31102..33534)
                     /gene="metL"
                     /locus_tag="NBI74_000447"
     CDS             complement(31102..33534)
                     /gene="metL"
                     /locus_tag="NBI74_000447"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000110794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase II"
                     /protein_id="HCD1930823.1"
                     /translation="MSVIAQAGAKGRQLHKFGGSSLADVKCYLRVAGIMAEYSQPDDM
                     MVVSAAGSTTNQLINWLKLSQTDRLSAHQVQQTLRRYQCDLISGLLPAEEADNLISAF
                     VSDLERLAALLDSGINDAVYAEVVGHGEVWSARLMSAVLNQQGLPSAWLDAREFLRAE
                     RAAQPQVDEGLSYPLLQQLLVQHPGKRLVVTGFISRNNAGETVLLGRNGSDYSATQIG
                     ALAGVSRVTIWSDVAGVYSADPRKVKDACLLPLLRLDEASELARLAAPVLHARTLQPV
                     SGSEIDLQLRCSYTPDQGSTRIERVLASGTGARIVTSHDDVCLIEFQVPASQDFKLAH
                     KEIDQILKRAQVRPLAVGVHNDRQLLQFCYTSEVADSALKILDEAGLPGELRLRQGLA
                     LVAMVGAGVTRNPLHCHRFWQQLKGQPVEFTWQSDDGISLVAVLRTGPTESLIQGLHQ
                     SVFRAEKRIGLVLFGKGNIGSRWLELFAREQSTLSARTGFEFVLAGVVDSRRSLLSYD
                     GLDASRALAFFNDEAVEQDEESLFLWMRAHPYDDLVVLDVTASQQLADQYLDFASHGF
                     HVISANKLAGASDSNKYRQIHDAFEKTGRHWLYNATVGAGLPINHTVRDLIDSGDTIL
                     SISGIFSGTLSWLFLQFDGSVPFTELVDQAWQQGLTEPDPRDDLSGKDVMRKLVILAR
                     EAGYNIEPDQVRVESLVPAHCEGGSIDHFFENGDELNEQMVQRLEAAREMGLVLRYVA
                     RFDANGKARVGVEAVREDHPLASLLPCDNVFAIESRWYRDNPLVIRGPGAGRDVTAGA
                     IQSDINRLAQLL"
     gene            complement(33537..34697)
                     /gene="metB"
                     /locus_tag="NBI74_000448"
     CDS             complement(33537..34697)
                     /gene="metB"
                     /locus_tag="NBI74_000448"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HCD1930824.1"
                     /translation="MTRKQATIAVRSGLNDDEQYGCVVPPIHLSSTYNFTGFNEPRAH
                     DYSRRGNPTRDVVQRALAELEGGAGAVLTNTGMSAIHLVTTVFLKPGDLLVAPHDCYG
                     GSYRLFDSLAKRGCYRVLFVDQSDEQALRAALAEKPKLVLVESPSNPLLRVVDIAKIC
                     HLAREVGAVSVVDNTFLSPALQNPLALGADLVLHSCTKYLNGHSDVVAGVVIAKDPDV
                     VTELAWWANNIGVTGGAFDSYLLLRGLRTLVPRMELAQRNAQAIVKYLQTQPLVKKLY
                     HPSLPENQGHEIAARQQKGFGAMLSFELDGDEQTLRRFLGGLSLFTLAESLGGVESLI
                     SHAATMTHAGMAPEARAAAGISETLLRISTGIEDGEDLIADLENGFRAANKG"
     gene            34974..35291
                     /gene="metJ"
                     /locus_tag="NBI74_000449"
     CDS             34974..35291
                     /gene="metJ"
                     /locus_tag="NBI74_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015855437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="met regulon transcriptional regulator MetJ"
                     /protein_id="HCD1930825.1"
                     /translation="MAEWSGEYISPYAEHGKKSEQVKKITVSIPLKVLKILTDERTRR
                     QVNNLRHATNSELLCEAFLHAFTGQPLPDDADLRKERSDEIPEAAKEIMREMGINPET
                     WEY"
     gene            35351..35959
                     /locus_tag="NBI74_000450"
     CDS             35351..35959
                     /locus_tag="NBI74_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiX family permuted papain-like enzyme"
                     /protein_id="HCD1930826.1"
                     /translation="MKIRLLIPGLLVSVPAFAWQPQTGDIIFQISRSSQSKAIQLATH
                     SDYSHTGMLVIRNKKPYVFEAVGPVKYTPLKQWIAHGEDSKYIVRRVKGELNAEQQQK
                     LAQTAKRYLGKPYDSSFSWSDDRQYCSEVVWKVYQNALGMRVGEQQKLKEFDLSNPLV
                     QAKLKERYGKNIPLEETVVSPQAVFDAPQLTTVAKEWPLFSW"
     gene            complement(36020..36232)
                     /gene="rpmE"
                     /locus_tag="NBI74_000451"
     CDS             complement(36020..36232)
                     /gene="rpmE"
                     /locus_tag="NBI74_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HCD1930827.1"
                     /translation="MKKDIHPKYEEITASCSCGNVMKIRSTVGHDLNLDVCSKCHPFF
                     TGKQRDVATGGRVDRFNKRFNIPGSK"
     gene            36435..38633
                     /gene="priA"
                     /locus_tag="NBI74_000452"
     CDS             36435..38633
                     /gene="priA"
                     /locus_tag="NBI74_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HCD1930828.1"
                     /translation="MPVAHVALPVPLPRTFDYLLPEGMTVKAGSRVRVPFGKQQERIG
                     VVVSVSDVSELPLNELKAVVEVLDVEPVFTHSVWRLLLWAADYYHHPIGDVLFHALPI
                     LLRQGRPAANAPMWYWFATEQGQAVDLNSLKRSPKQQQALAALRQGKIWRDQVATLEF
                     NDAALQALRKKGLCDLASETPEFSDWRTNYAVSGERLRLNTEQATAVGAIHSAADTFS
                     AWLLAGVTGSGKTEVYLSVLENVLAQGKQALVMVPEIGLTPQTIARFRERFNAPVEVL
                     HSGLNDSERLSAWLKAKNGEAAIVIGTRSALFTPFKNLGVIVIDEEHDSSYKQQEGWR
                     YHARDLAVYRAHSEQIPIILGSATPALETLCNVQQKKYRLLRLTRRAGNARPAIQHVL
                     DLKGQKVQAGLAPALITRMRQHLQADNQVILFLNRRGFAPALLCHDCGWIAECPRCDH
                     YYTLHQAQQHLRCHHCDSQRPVPRQCPSCGSTHLVPVGLGTEQLEQTLAPLFPGVPIS
                     RIDRDTTSRKGALEQQLAEVHRGGARILIGTQMLAKGHHFPDVTLVALLDVDGALFSA
                     DFRSAERFAQLYTQVAGRAGRAGKQGEVVLQTHHPEHPLLQTLLYKGYDDFAEQALAE
                     RRMMQLPPWTSHVIVRAEDHNNQHAPLFLQQLRNLILASPLADDKLWVLGPVPALAPK
                     RGGRWRWQILLQHPSRVRLQHIINGTLALINTIPDSRKVKWVLDVDPIEG"
     gene            38789..39814
                     /gene="cytR"
                     /locus_tag="NBI74_000453"
     CDS             38789..39814
                     /gene="cytR"
                     /locus_tag="NBI74_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CytR"
                     /protein_id="HCD1930829.1"
                     /translation="MKAKKQETAATMKDVALKAKVSTATVSRALMNPDKVSQATRNRV
                     EKAAREVGYLPQPMGRNVKRNESRTILVIVPDICDPFFSEIIRGIEVTAANHGYLVLI
                     GDCAHQNQQEKTFIDLIITKQIDGMLLLGSRLPFDASIEEQRNLPPMVMANEFAPELE
                     LPTVHIDNLTAAFDAVNYLYEQGHKRIGCIAGPEEMPLCHYRLQGYVQALRRCGIMVD
                     PQYIARGDFTFEAGSRAMQQLLDLPQPPTAVFCHSDVMALGALSQAKRQGLKVPEDLS
                     IIGFDNIDLTQFCDPPLTTIAQPRYEIGREAMLLLLDQMQGQHVGSGSRLMDCELIIR
                     GSTRALP"
     gene            39906..40865
                     /gene="ftsN"
                     /locus_tag="NBI74_000454"
     CDS             39906..40865
                     /gene="ftsN"
                     /locus_tag="NBI74_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsN"
                     /protein_id="HCD1930830.1"
                     /translation="MAQRDYVRRSQPAPSRRKKSTSRKKQRNLPAVSPAMVAIAAAVL
                     VTFIGGLYFITHHKKEESETLQSQKVTGNGLPPKPEERWRYIKELESRQPGVRAPTEP
                     SAGGEVKTPEQLTPEQRQLLEQMQADMRQQPTQLVEVPWNEQTPEQRQQTLQRQRQAQ
                     QLAEQQRLAQQSRTTEQSWQQQTRTSQAAPVQAQPRQSKPASTQQPYQDLLQTPAHTT
                     AQSKPQQAAPVARAADAPKPTAEKKDERRWMVQCGSFRGAEQAETVRAQLAFEGFDSK
                     ITTNNGWNRVVIGPVKGKENADSTLNRLKMAGHTNCIRLAAGG"
     gene            40958..41488
                     /gene="hslV"
                     /locus_tag="NBI74_000455"
     CDS             40958..41488
                     /gene="hslV"
                     /locus_tag="NBI74_000455"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HCD1930831.1"
                     /translation="MTTIVSVRRNGHVVIAGDGQATLGNTVMKGNVKKVRRLYNDKVI
                     AGFAGGTADAFTLFELFERKLEMHQGHLVKAAVELAKDWRTDRMLRKLEALLAVADET
                     ASLIITGNGDVVQPENDLIAIGSGGPYAQAAARALLENTELSAREIAEKALDIAGDIC
                     IYTNHFHTIEELSYKA"
     gene            41498..42829
                     /gene="hslU"
                     /locus_tag="NBI74_000456"
     CDS             41498..42829
                     /gene="hslU"
                     /locus_tag="NBI74_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019841241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase ATPase subunit"
                     /protein_id="HCD1930832.1"
                     /translation="MSEMTPREIVSELDKHIIGQDNAKRSVAIALRNRWRRMQLNEEL
                     RHEVTPKNILMIGPTGVGKTEIARRLAKLANAPFIKVEATKFTEVGYVGKEVDSIIRD
                     LTDAAVKMVRVQAIEKNRYRAEELAEERILDVLIPPAKNNWGQTEQQQEPSAARQAFR
                     KKLREGQLDDKEIEIDLAAAPMGVEIMAPPGMEEMTSQLQSMFQNLGGQKQKARKLKI
                     KDAMKLLIEEEAAKLVNPEELKQDAIDAVEQHGIVFIDEIDKICKRGESSGPDVSREG
                     VQRDLLPLVEGCTVSTKHGMVKTDHILFIASGAFQIAKPSDLIPELQGRLPIRVELQA
                     LTTSDFERILTEPNASITVQYKALMATEGVNIEFTDSGIKRIAEAAWQVNESTENIGA
                     RRLHTVLERLMEEISYDASDLSGQTITIDADYVSKHLDALVADEDLSRFIL"
     gene            42896..43822
                     /gene="menA"
                     /locus_tag="NBI74_000457"
     CDS             42896..43822
                     /gene="menA"
                     /locus_tag="NBI74_000457"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HCD1930833.1"
                     /translation="MTEQQISRTQAWLESLRPKTLPLAFAAIIVGTALAWWQGHFDPL
                     VALLALITAGLLQILSNLANDYGDAVKGSDKPDRIGPLRGMQKGVITQQEMKRALIIT
                     VVLICLSGLALVAVACHTLADFVGFLILGGLSIIAAITYTVGNRPYGYIGLGDISVLV
                     FFGWLSVMGSWYLQAHTLIPALILPATACGLLATAVLNINNLRDINSDRENGKNTLVV
                     RLGAVNARRYHACLLMGSLVCLALFNLFSLHSLWGWLFLLAAPLLVKQARYVMREMDP
                     VAMRPMLERTVKGALLTNLLFVLGIFLSQWAA"
     gene            43915..44400
                     /gene="rraA"
                     /locus_tag="NBI74_000458"
     CDS             43915..44400
                     /gene="rraA"
                     /locus_tag="NBI74_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E activity regulator RraA"
                     /protein_id="HCD1930834.1"
                     /translation="MKYDTSELCDIYQEDVNVVEPLFSNFGGRASFGGQIITVKCFED
                     NGLLYDLLEQNGRGRVLVVDGGGSVRRALVDAELARLAVQNEWEGLVIYGAVRQVDDL
                     EELDIGIQAMAAIPVGAAGEGIGESDVRVNFGGVTFFSGDHLYADNTGIILSEDPLDI
                     E"
     gene            complement(44485..44730)
                     /gene="zapB"
                     /locus_tag="NBI74_000459"
     CDS             complement(44485..44730)
                     /gene="zapB"
                     /locus_tag="NBI74_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring assembly protein ZapB"
                     /protein_id="HCD1930835.1"
                     /translation="MTMSLEVFEKLEAKVQQAIDTITLLQMEIEELKEKNNSLSQEVQ
                     NAQHQREELERENNHLKEQQNGWQERLQALLGRMEEV"
     gene            45155..46000
                     /gene="glpF"
                     /locus_tag="NBI74_000460"
     CDS             45155..46000
                     /gene="glpF"
                     /locus_tag="NBI74_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol uptake facilitator protein GlpF"
                     /protein_id="HCD1930836.1"
                     /translation="MSQTSTLKGQCIAEFLGTGLLIFFGVGCVAALKVAGASFGQWEI
                     SVIWGLGVAMAIYLTAGVSGAHLNPAVTIALWLFACFDKRKVIPFIVSQVAGAFCAAA
                     LVYGLYYNLFFDFEQTHHIVRGSVESVDLAGTFSTYPNPHINFVQAFAVEMVITAILM
                     GLILALTDDGNGVPRGPLAPLLIGLLIAVIGASMGPLTGFAMNPARDFGPKVFAWLAG
                     WGNVAFTGGRDIPYFLVPLFGPIVGAIVGAFAYRKLIGRHLPCDICVVEEKETTTPSE
                     QKASL"
     gene            46023..47531
                     /gene="glpK"
                     /locus_tag="NBI74_000461"
     CDS             46023..47531
                     /gene="glpK"
                     /locus_tag="NBI74_000461"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HCD1930837.1"
                     /translation="MTEKKYIVALDQGTTSSRAVVMDHDANIISVSQREFEQIYPKPG
                     WVEHDPMEIWATQSSTLVEVLAKADISSDQIAAIGITNQRETTIVWEKETGKPIYNAI
                     VWQCRRTAEICEHLKRDGMEEYIRNNTGLVIDPYFSGTKVKWILDHVEGSRERARRGE
                     LLFGTVDTWLIWKMTQGRVHVTDYTNASRTMLFNIHTLDWDDKMLEVLDIPREMLPEV
                     RRSSEVYGQTNIGGKGGTRIPISGIAGDQQAALFGQLCVKEGMAKNTYGTGCFMLMNT
                     GEKAVKSENGLLTTIACGPTGEVNYALEGAVFMAGASIQWLRDEMKLINDAYDSEYFA
                     TKVQNTNGVYVVPAFTGLGAPYWDPYARGAIFGLTRGVNANHIIRATLESIAYQTRDV
                     LEAMQADSGIRLHALRVDGGAVANNFLMQFQSDILGTRVERPEVREVTALGAAYLAGL
                     AVGFWQNLDELQEKAVIEREFRPGIETTERNYRYAGWKKAVKRAMAWEEHDE"
     gene            47702..48712
                     /gene="glpX"
                     /locus_tag="NBI74_000462"
     CDS             47702..48712
                     /gene="glpX"
                     /locus_tag="NBI74_000462"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HCD1930838.1"
                     /translation="MRRELAIEFSRVTESAALAGYKWLGRGDKNTADGAAVNAMRIML
                     NQVNIDGTIVIGEGEIDEAPMLYIGEKVGTGRGDAVDIAVDPIEGTRMTAMGQANALA
                     VLAVGDKGCFLNAPDMYMEKLIVGPGAKGTIDLNLPLADNLRNVAAALGKPLSELTVT
                     ILAKPRHDAVIAEMQQLGVRVFAIPDGDVAASILTCMPDSEVDVLYGIGGAPEGVVSA
                     AVIRALDGDMNGRLLARHDVKGDNEENRRIGEQELARCKAMGIEAGKVLRLGDMARSD
                     NVIFSATGITKGDLLEGISRKGNIATTETLLIRGKSRTIRRIQSIHYLDRKDPEMQVH
                     IL"
     gene            48809..49555
                     /gene="fpr"
                     /locus_tag="NBI74_000463"
     CDS             48809..49555
                     /gene="fpr"
                     /locus_tag="NBI74_000463"
                     /EC_number="1.18.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP(+) reductase"
                     /protein_id="HCD1930839.1"
                     /translation="MADWVTGKVTKVQNWTDALFSLTVHAPVLPFTAGQFTKLGLEIE
                     GERVQRAYSYVNSPDNPDLEFYLVTVPDGKLSPRLAALKPGDEVQVVSEAAGFFVLDE
                     VPDCETLWMLATGTAIGPYLSILQLGKDLERFKNMVLVHAARYAADLSYLPLMQELEK
                     RYEGKLRIQTVVSRETAAGSLTGRIPALIESGELESAVGLPMNKETSHVMLCGNPQMV
                     RDTQQLLKETRQMTKHLRRRPGHMTAEHYW"
     gene            complement(49560..49988)
                     /gene="uspD"
                     /locus_tag="NBI74_000464"
     CDS             complement(49560..49988)
                     /gene="uspD"
                     /locus_tag="NBI74_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspD"
                     /protein_id="HCD1930840.1"
                     /translation="MAYKHIGVAISGNEEDALLVNKALELARHNDAHLTLIHIDDGLS
                     ELYPGIYFPATEDILQLLKNKSDNKLYKLTKNIQWPKTKLRIERGEMPETLLEIMQKE
                     QCDLLVCGHHHSFINRLMPAYRGMINKLSADLLIVPFIDK"
     gene            complement(50015..50314)
                     /locus_tag="NBI74_000465"
     CDS             complement(50015..50314)
                     /locus_tag="NBI74_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF406 domain-containing protein"
                     /protein_id="HCD1930841.1"
                     /translation="MKDVVDKCSTKGCAIDIGTVIDNDNCTSKFSRFFATREEAESFM
                     TKLKELAAAASSADEGASVAYKIKDLEGQVELDAAFTFSCQAEMIIFELSLRSLA"
     gene            complement(50526..50966)
                     /locus_tag="NBI74_000466"
     CDS             complement(50526..50966)
                     /locus_tag="NBI74_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HCD1930842.1"
                     /translation="MTIQQWLFSFKGRIGRRDFWIWIGLWFAGMLVLFSLAGKNLLDI
                     QTAAFCLVCLLWPTAAVTVKRLHDRGRSGAWAFLMIVAWMLLAGNWAILPGVWQWAVG
                     RFIPTLILVMMLIDLGAFVGTQGENKYGKDTQDVKYKADNKSSN"
     gene            51067..51666
                     /locus_tag="NBI74_000467"
     CDS             51067..51666
                     /locus_tag="NBI74_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiQ family protein"
                     /protein_id="HCD1930843.1"
                     /translation="MKPGCTLFILLCSALTVTTTAHAQTPDTATTAPYLLAGAPTFDL
                     SISQFREDFNSQNPSLPLNEFRAIDSSPDKANLTRAASKINENLYASTALERGTLKIK
                     SIQITWLPIQGPEQKAAKAKAQAYMAAVIRTLTPLMTKTQSQKKLQSLLTAGKNKRYY
                     TETEGALRYVVADNGEKGLTFAVEPIKLALSESLEGLNK"
     gene            51774..52541
                     /gene="tpiA"
                     /locus_tag="NBI74_000468"
     CDS             51774..52541
                     /gene="tpiA"
                     /locus_tag="NBI74_000468"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HCD1930844.1"
                     /translation="MRHPLVMGNWKLNGSRHMVHELVSNLRKELAGVAGCAVAIAPPE
                     MYIDMAKREAEGSHIMLGAQNVDLNLSGAFTGETSAAMLKDIGAQYIIIGHSERRTYH
                     KESDELIAKKFAVLKEQGLTPVLCIGETEAENEAGKTEEVCARQIDAVLKTQGAAAFE
                     GAVIAYEPVWAIGTGKSATPAQAQAVHKFIRDHIAKVDANIAEQVIIQYGGSVNASNA
                     AELFAQPDIDGALVGGASLKADAFAVIVKAAEAAKQA"
     gene            complement(52596..53351)
                     /gene="cdh"
                     /locus_tag="NBI74_000469"
     CDS             complement(52596..53351)
                     /gene="cdh"
                     /locus_tag="NBI74_000469"
                     /EC_number="3.6.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol diphosphatase"
                     /protein_id="HCD1930845.1"
                     /translation="MKKAGLLFLVMIVIAVVTAGIGYWKLTGEESDTLRKIVLEQCLP
                     NQQENQNPSPCAEVKPNAGYVVLKDRHGPLQYLLMPTYRINGTESPLLTDPSTPNFFW
                     LAWQARDFMSQKYGQPVPDRAVSLAINSRTGRTQNHFHIHISCIRPDVREQLDNNLAN
                     ISSRWLPLPGGLRGHEYLARRVTESELVQRSPFMMLAEEVPEAREHMGSYGLAMVRQS
                     DNSFVLLATQRNLLTLNRASAEEIQDHQCEILR"
     gene            complement(53458..54447)
                     /gene="sbp"
                     /locus_tag="NBI74_000470"
     CDS             complement(53458..54447)
                     /gene="sbp"
                     /locus_tag="NBI74_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter
                     substrate-binding protein Sbp"
                     /protein_id="HCD1930846.1"
                     /translation="MKKWGVGLTFLLVATSVMAKDIQLLNVSYDPTRELYEQYNKAFS
                     AHWKQQTGDNVVIRQSHGGSGKQATSVINGIEADVVTLALAYDVDAIAERGRIDKEWI
                     KRLPDNSAPYTSTIVFLVRKGNPKQIHDWNDLIKPGVSVITPNPKSSGGARWNYLAAW
                     GYALHHNNNDQAKAQDFVRALYKNVEVLDSGARGSTNTFVERGIGDVLIAWENEALLA
                     ANELGKDKFEIVTPSESILAEPTVSVVDKVVEKKGTKEVAEAYLKYLYSPEGQEIAAK
                     NYYRPRDAEVAKKYENAFPKLKLFTIDEEFGGWTKAQKEHFANGGTFDQISKR"
     gene            complement(54767..55729)
                     /gene="pfkA"
                     /locus_tag="NBI74_000471"
     CDS             complement(54767..55729)
                     /gene="pfkA"
                     /locus_tag="NBI74_000471"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014072359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HCD1930847.1"
                     /translation="MIKKIGVLTSGGDAPGMNAAIRGVVRSALTEGLEVMGIYDGYLG
                     LYEDRMVQLDRYSVSDMINRGGTFLGSARFPEFRDENIRAVAIENLKKRGIDALVVIG
                     GDGSYMGAMRLTEMGFPCIGLPGTIDNDIKGTDYTIGFFTALSTVVEAIDRLRDTSSS
                     HQRISVVEVMGRYCGDLTLAAAIAGGCEFVVVPEVEFSREDLVNEIKAGIAKGKKHAI
                     VAITEHMCDVDELAHFIEKETGRETRATVLGHIQRGGSPVPYDRILASRMGAYAIDLL
                     LAGYGGRCVGIQNEQLVHHDIIDAIENMKRPFKGDWLDCAKKLY"
     gene            complement(55910..56812)
                     /gene="fieF"
                     /locus_tag="NBI74_000472"
     CDS             complement(55910..56812)
                     /gene="fieF"
                     /locus_tag="NBI74_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709720.2"
                     /note="FieF, a metal efflux transporter, is a member of
                     the CDF (cation diffusion facilitator) family of
                     transporters; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family cation-efflux transporter FieF"
                     /protein_id="HCD1930848.1"
                     /translation="MNQSYGRLVSRAAIAATAMASLLLLIKIFAWWYTGSVSILAALV
                     DSLVDIGASLTNLLVVRYSLQPADDNHSFGHGKAESLAALAQSMFISGSALFLFLTGI
                     QHLVSPTPMTDPGVGVIVTIVALICTIILVSFQRWVVRRTQSQAVRADMLHYQSDVMM
                     NGAILLALGLSWYGWHRADALFALGIGIYILYSALRMGYEAVQSLLDRALSDEERQEI
                     IDIVTSWPGVSGAHDLRTRQSGPTRFIQIHLEMEDSLPLVQAHMVADQVEQAILRRFP
                     GSDVIIHQDPCSVVPREGKRSMLS"
     gene            complement(56961..57461)
                     /gene="cpxP"
                     /locus_tag="NBI74_000473"
     CDS             complement(56961..57461)
                     /gene="cpxP"
                     /locus_tag="NBI74_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell-envelope stress modulator CpxP"
                     /protein_id="HCD1930849.1"
                     /translation="MRIVTAAVMASTLAVSSLSHAAEVGSGDNWHPGEELTQRSTQSH
                     MFDGISLTEHQRQQMRDLMQQARHEQPPVNVSELETMHRLVTAENFDENAVRAQAEKM
                     ANEQIARQVEMAKVRNQMYRLLTPEQQAVLNEKHQQRMEQLRDVTQWQKSSSLKLLSS
                     SNSRSQ"
     gene            57611..58309
                     /gene="cpxR"
                     /locus_tag="NBI74_000474"
     CDS             57611..58309
                     /gene="cpxR"
                     /locus_tag="NBI74_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response regulator transcription
                     factor CpxR"
                     /protein_id="HCD1930850.1"
                     /translation="MNKILLVDDDRELTSLLKELLEMEGFNVIVAHDGEQALDLLDDS
                     IDLLLLDVMMPKKNGIDTLKALRQTHQTPVIMLTARGSELDRVLGLELGADDYLPKPF
                     NDRELVARIRAILRRSHWSEQQQNNDNGSPTLEVDALSLNPGRQEATFDGQTLELTGT
                     EFTLLYLLAQHLGQVVSREHLSQEVLGKRLTPFDRAIDMHISNLRRKLPDRKDGHPWF
                     KTLRGRGYLMVSAS"
     gene            58306..59679
                     /gene="cpxA"
                     /locus_tag="NBI74_000475"
     CDS             58306..59679
                     /gene="cpxA"
                     /locus_tag="NBI74_000475"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress sensor histidine kinase CpxA"
                     /protein_id="HCD1930851.1"
                     /translation="MIGSLTARIFAIFWLTLALVLMLVLMLPKLDSRQMTELLDSEQR
                     QGLMIEQHVEAELANDPPNDLMWWRRLFRAIDKWAPPGQRLLLVTTEGRVIGAERSEM
                     QIIRNFIGQADNADHPQKKKYGRVELVGPFSVRDGEDNYQLYLIRPASSSQSDFINLL
                     FDRPLLLLIVTMLVSTPLLLWLAWSLAKPARKLKNAADEVAQGNLRQHPELEAGPQEF
                     LAAGASFNQMVTALERMMTSQQRLLSDISHELRTPLTRLQLGTALLRRRSGESKELER
                     IETEAQRLDSMINDLLVMSRNQQKNALVSETIKANQLWSEVLDNAAFEAEQMGKSLTV
                     NFPPGPWPLYGNPNALESALENIVRNALRYSHTKIEVGFAVDKDGITITVDDDGPGVS
                     PEDREQIFRPFYRTDEARDRESGGTGLGLAIVETAIQQHRGWVKAEDSPLGGLRLVIW
                     LPLYKRS"
     gene            complement(59784..60458)
                     /gene="yiiM"
                     /locus_tag="NBI74_000476"
     CDS             complement(59784..60458)
                     /gene="yiiM"
                     /locus_tag="NBI74_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-N-hydroxylaminopurine resistance protein"
                     /protein_id="HCD1930852.1"
                     /translation="MRYPVDVYTGKIQAYPEGKPSAIAKIQVDGELMLTDLGLEGDEQ
                     AEKKIHGGPDRALCHYPREHYLYWAREFPEQAELFVAPAFGENLSTDGLTESNVYIGD
                     IFRWGEALIQVSQPRSPCYKLNYHFDISDIAQLMQNTGKVGWLYSVIAPGLVSADAPL
                     ELVSRVSDVTVQEAAAIAWHMPFDDDQYHRLLSAAGLSKSWTRTMQKRRLSGKIEDFS
                     RRLWGK"
     gene            complement(60607..61590)
                     /gene="kdgT"
                     /locus_tag="NBI74_000477"
     CDS             complement(60607..61590)
                     /gene="kdgT"
                     /locus_tag="NBI74_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxygluconate transporter"
                     /protein_id="HCD1930853.1"
                     /translation="MQIKRSIEKIPGGMMLVPLFLGALCHTFSPEAGKYFGSFTNGMI
                     TGTVPILAVWFFCMGASIKLSATGTVLRKSGTLVVTKIAVAWVVAAIASRIIPEHGVE
                     VGFFAGLSTLALVAAMDMTNGGLYASIMQQYGTKEEAGAFVLMSLESGPLMTMIILGT
                     AGIASFEPHVFVGAVLPFLVGFALGNLDPELREFFSKAVQTLIPFFAFALGNTIDLTV
                     IAQTGLLGILLGVAVIIVTGIPLIIADKLIGGGDGTAGIAASSSAGAAVATPVLIAEM
                     VPAFKPMAPAATSLVATAVIVTSILVPILTSIWSRKVKARAAKIEILGTVK"
     gene            complement(61850..62470)
                     /gene="sodA"
                     /locus_tag="NBI74_000478"
     CDS             complement(61850..62470)
                     /gene="sodA"
                     /locus_tag="NBI74_000478"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010345169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Mn]"
                     /protein_id="HCD1930854.1"
                     /translation="MSYTLPSLPYAYDALEPHFDKQTMEIHHTKHHQTYVNNANAALE
                     SLPEFANLPVEELITKLDQLPADKKTVLRNNAGGHANHSLFWKGLKKGTTLQGDLKAA
                     IERDFGSVDNFKAEFEKAAASRFGSGWAWLVQKGDKLAVVSTANQDSPLMGEAISGAS
                     GFPILGLDVWEHAYYLKFQNRRPDYIKEFWNVVNWDEAAARFAAKK"
     gene            62755..63789
                     /gene="rhaT"
                     /locus_tag="NBI74_000479"
     CDS             62755..63789
                     /gene="rhaT"
                     /locus_tag="NBI74_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose/proton symporter RhaT"
                     /protein_id="HCD1930855.1"
                     /translation="MSNAITMGIFWHLIGAASAACFYAPFKKVKKWSWETMWSVGGIV
                     SWIILPWAISALLLPNFWAYYSSFSLSTLLPVFLFGAMWGIGNINYGLTMRYLGMSMG
                     IGIAIGITLIVGTLMTPIINGNFDVLINTEGGRMTLLGVLVALIGVGIVTRAGQLKER
                     KMGIKAEEFNLKKGLVLAVMCGIFSAGMSFAMNAAKPMHEAAAALGVDPLYVALPSYV
                     VIMGGGAIINLGFCFIRLAKVKDLSLKADFSLAKPLIIHNVLLSALGGLMWYLQFFFY
                     AWGHARIPAQYDYISWMLHMSFYVLCGGIVGLVLKEWNNAGRRPVTVLSLGCVVIIVA
                     ANIVGIGMAN"
     gene            complement(63786..64652)
                     /gene="rhaR"
                     /locus_tag="NBI74_000480"
     CDS             complement(63786..64652)
                     /gene="rhaR"
                     /locus_tag="NBI74_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaR"
                     /protein_id="HCD1930856.1"
                     /translation="MAEVATVAHQLKLLKDDFFASDQQAVAVADRYPQDVFAEHTHDF
                     CELVIVWRGNGLHVLNDRPYRITRGDLFYIHADDKHSYASVNDLVLQNIIYCPERLKL
                     NLDWQGAIPGFSASAGQPHWRLGSVGMAQARQVIGQLEHESSQHVSFANEMAELLFGQ
                     LVMLLNRHRYTSDSLPPTSSETLLDKLITRLAASLKSPFALDKFCDEASCSERVLRQQ
                     FRQQTGMTINQYLRQVRVCHAQYLLQHSRLLISDISTECGFEDSNYFSVVFTRETGMT
                     PSQWRHLNSQKD"
     gene            complement(64708..65544)
                     /gene="rhaS"
                     /locus_tag="NBI74_000481"
     CDS             complement(64708..65544)
                     /gene="rhaS"
                     /locus_tag="NBI74_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaS"
                     /protein_id="HCD1930857.1"
                     /translation="MTVLHSVDFFPSGNASVAIEPRLPQADFPEHHHDFHEIVIVEHG
                     TGIHVFNGQPYTITGGTVCFVRDHDRHLYEHTDNLCLTNVLYRSPDRFQFLAGLNQLL
                     PQEQDGQYPSHWRVNHSVLQQVRQLVAQMEQQEGENDLPSTASREILFMQLLLLLRKS
                     SLQENLENSASRLNLLLAWLEDHFADEVNWDAVADQFSLSLRTLHRQLKQQTGLTPQR
                     YLNRLRLMKARHLLRHSEASVTDIAYRCGFSDSNHFSTLFRREFNWSPRDIRQGRDGF
                     LQ"
     gene            65832..67301
                     /gene="rhaB"
                     /locus_tag="NBI74_000482"
     CDS             65832..67301
                     /gene="rhaB"
                     /locus_tag="NBI74_000482"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HCD1930858.1"
                     /translation="MTFRNCVAVDLGASSGRVMLARYERECRSLTLREIHRFNNGLHS
                     QNGYVTWNVDSLESAIRLGLNKVCEEGIRIDSIGIDTWGVDFVLLDQHGQRVGLPVAY
                     RDSRTNGLMAQAQQQLGKRDIYQRSGIQFLPFNTIYQLRALTEQQPELIPHIAHALLI
                     PDYFSYRLTGKMNWEYTNATTTQLVNINSDDWDESLLAWSGANKAWFGRPTHPGNVIG
                     HWICPQGNEIPVVAVASHDTASAVIASPLNGSRAAYLSSGTWSLMGFESQTPFTNDTA
                     LAANITNEGGAEGRYRVLKNIMGLWLLQRVLQERQINDLPALIAATQALPACRFIINP
                     NDDRFINPDEMCSEIQAACRETAQPIPESDAELARCIFDSLALLYADVLHELAQLRGE
                     DFSQLHIVGGGCQNTLLNQLCADACGIRVIAGPVEASTLGNIGIQLMTLDELNNVDDF
                     RQVVSTTANLTTFTPNPDSEIAHYVAQIHSTRQTKELCA"
     gene            67298..68557
                     /gene="rhaA"
                     /locus_tag="NBI74_000483"
     CDS             67298..68557
                     /gene="rhaA"
                     /locus_tag="NBI74_000483"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HCD1930859.1"
                     /translation="MTTQLEQAWELAKQRFAAVGIDVEEALRQLDRLPVSMHCWQGDD
                     VSGFENPEGSLTGGIQATGNYPGKARNASELRADLEQAMRLIPGPKRLNLHAIYLESD
                     TPVSRDQIKPEHFKNWVEWAKANQLGLDFNPSCFSHPLSADGFTLSHADDRIRQFWID
                     HCKASRRVSAYFGEQLGTPSVMNIWIPDGMKDITVDRLAPRQRLLAALDEVISEKLNP
                     AHHIDAVESKLFGIGAESYTVGSNEFYLGYATSRQTALCLDAGHFHPTEVISDKISAA
                     MLYVPQLLLHVSRPVRWDSDHVVLLDDETQAIASEIVRHDLFDRVHIGLDFFDASINR
                     IAAWVIGTRNMKKALLRALLEPTAELRKLESAGDYTARLALLEEQKSLPWQAVWEMYC
                     QRHDTPAGSEWLENVRTYEKKILSRRG"
     gene            68640..69464
                     /gene="rhaD"
                     /locus_tag="NBI74_000484"
     CDS             68640..69464
                     /gene="rhaD"
                     /locus_tag="NBI74_000484"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HCD1930860.1"
                     /translation="MQNITQSWFVQGMIKATTDAWLKGWDERNGGNLTLRLDDADIAP
                     YHDNFHQQPRYIPLSQPMPLLANTPFIVTGSGKFFRNVQLDPAANLGIVKVDSDGAGY
                     HILWGLTNEAVPTSELPAHFLSHCERIKATNGKDRVIMHCHATNLIALTYVLENDTAV
                     FTRQLWEGSTECLVVFPDGVGILPWMVPGTDEIGQATAQEMQKHSLVLWPFHGVFGSG
                     PTLDETFGLIDTAEKSAQVLVKVYSMGGMKQTISREELIALGQRFGVTPLASALAL"
     gene            69474..69788
                     /gene="rhaM"
                     /locus_tag="NBI74_000485"
     CDS             69474..69788
                     /gene="rhaM"
                     /locus_tag="NBI74_000485"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HCD1930861.1"
                     /translation="MIRKAFVMQVNPDAHEEYQRRHNPIWPELEAVLKSHGAHNYAIY
                     LDKARNLLFATIEIESEERWNAVASTDVCQRWWKYMTDVMPANADNSPVSSELQEVFY
                     LP"
     gene            complement(69829..71223)
                     /locus_tag="NBI74_000486"
     CDS             complement(69829..71223)
                     /locus_tag="NBI74_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate porin"
                     /protein_id="HCD1930862.1"
                     /translation="MKKSTLSLAIGLLLACSTGMAKTQHLTLEQRMALLEERLEVAEM
                     RAAKAEGQVKQLQTQQAAEIREIKTAQGNTPVNGQSTTESEKKNATPPNLLLSGYGDL
                     KIYGDVEFNMDAESNHGLLAMTNADVNSDPTNEQWNLNGRILLGFDGMRKLDNGYFAG
                     FSAQPLGDMHGSVNIDDAVFFFGKENDWKVKVGRFEAYDMFPLNQDTFVEHSGNTAND
                     LYDDGSGYIYMMKEGRGRSNAGGNFLVSKQLDNWYFELNTLLEDGTSLYNDGNYHGRD
                     MEQQKNVAYLRPVIAWSPTEEFTVSAAMEANVVNNAYGYTDSKGNFVDQSDRTGYGMS
                     MTWNGLKTDPENGIVVNLNTAYLDANNEKDFTAGINALWKRFELGYIYAHNKIDEFSG
                     VVCDNDCWIDDEGTYNIHTIHASYQFANVMDMENFNIYLGTYYSILDSDGDKIHGDDS
                     DDRYGARVRFKYFF"
BASE COUNT        17404 a        19137 c        18177 g        16737 t
ORIGIN      
        1 tgttaccatt ctgttgcttt tatgtataag aacaggtaag ccctaccatg ccacattcct
       61 acgattacga tgccatagta ataggttccg gccccggcgg cgaaggcgct gcgatgggcc
      121 tggttaagca aggtgcgcgc gtcgcagtta tcgagcgtta tcaaaatgtt ggcggcggtt
      181 gcacccactg gggcaccatc ccgtcgaaag ctctccgtca cgccgtcagc cgcattatag
      241 aattcaatca aaacccacta tacagcgacc attcccgact gctccgctct tcttttgccg
      301 atatccttaa ccatgccgat aacgtgatta atcaacaaac gcgcatgcgt cagggatttt
      361 acgaacgtaa tcactgtgaa atattgcagg ggaacgctcg ctttgttgat gagcatacgt
      421 tggcgctgga ttgcccggac ggcagcgtcg aaacactaac tgctgaaaaa tttgttattg
      481 cctgcggttc tcgtccatat catccaacag atgttgattt cacccatcca cgcatttatg
      541 acagcgactc aattcttagc atgcaccacg aaccgcgcca tgtacttatt tacggtgctg
      601 gagtgatcgg ctgtgaatat gcgtcgatct tccgcggtat ggatgtaaaa gtggatctga
      661 tcaacacccg cgatcgcctg ctggcatttc tcgatcaaga gatgtcagat tctctctcct
      721 atcacttctg gaacagtggc gtagtgattc gtcacaacga agagtacgag aagatcgaag
      781 gctgtgatga cggtgtgatc atgcatctga agtcgggtaa aaaactgaaa gctgactgtc
      841 tgctctatgc caacggtcgc accggtaata ccgattcgct ggcgttacag aacattgggc
      901 tggaaactga cagtcgcgga cagctgaagg tcaacagcat gtatcagacc gcacagccgc
      961 acgtttacgc ggtgggcgac gtgattggtt atccgagcct ggcgtcggca gcctatgacc
     1021 aggggcgcat tgccgcgcag gcgctggtga aaggtgaagc caccgcacat ctgattgaag
     1081 atatccctac cggcatttac accatcccgg aaatcagctc tgtaggcaaa accgaacagc
     1141 agctgaccgc gatgaaagtg ccatatgaag taggccgcgc ccagtttaaa catctggcac
     1201 gtgcacaaat cgtcggcatg aacgtgggca cgctgaaaat tttgttccat cgggaaacaa
     1261 aagagattct gggtattcac tgctttggcg agcgcgctgc cgaaattatt catatcggtc
     1321 aggcgattat ggaacagaaa ggtggcggca acactattga gtacttcgtc aacaccacct
     1381 ttaactaccc gacgatggcg gaagcctatc gggtagctgc gctaaatggc ttaaaccgcc
     1441 tgttttaaca ctttatcgaa atggccatcc attcttgcgc ggatggcctc tgccagctgc
     1501 tcatagcggc tgcgcagcgg tgagccagga cgataaacca ggccaatagt gcggcgtggt
     1561 tccggcttaa tgcacggcag ataaacaacc ccatcgcgtt tgcgttccgg cggcacagcc
     1621 agcgcaggca gtaaagtgat cccgctacct gccgccacca tgttgcgcag agtttccagg
     1681 ctggtcgcgc ggaagtgtgt atcttcatcc gccccggctt caaaacagaa acccattgcc
     1741 tgatcgcgca aacagtgacc atcttccagc atcagcagtt tttcccctgc cagatcggcc
     1801 atcggtacgc attcgcggtt cgcccacggg tggtcttcat agatagccag caacattggc
     1861 tcatcaaaca acggcacttc aatgaatgct tcgctctctt tcaccagcgc gaggatcacg
     1921 caatcgagtt tgccgctgtc cagttgcgcc agtaactggt gggtctgtgc ttcatgcaga
     1981 tacatttcca gctttggaaa ggtctggtgc agcatcggga taatatgcgg tagcaggtac
     2041 ggtccaaccg tgggaatcaa accaatatgc agcggtccgg acatcgtctc gccctgctgg
     2101 cttgccatct ctttaaggac tttcacctca cgcagcacgg tacgcgcctg atccaccagc
     2161 agcattcccg cctgggtgaa taacacttta cggctggtcc gctccagcaa catcacgccc
     2221 agctcatctt ccagcttacg aatttgcccg ctaagcgtcg gctggctaac gtggcaggaa
     2281 tctgccgcac gccgaaaatg gcggtgttca gccaatgcca ccaggtactc aagatcacga
     2341 atattcatta tccatcctcc atcgccacga tagttcatgg cgataggtag aatagcaatg
     2401 aacgattatc cctatcaagc attctgactg ataattgctc acagaaacgg agcggcacct
     2461 cttttaaccc ttgaagtcac agcccgtttc gagagtttct cagctcgaat aactaaagcc
     2521 aacgtgaact tttgcggatc tccaggatcc gctttttttt gccataaaaa agcccagcga
     2581 taagccaggc tcaaatttat acatataaat gttcttaccc caaccgcgcc tgcgcaaaag
     2641 caatcgcctg cgccacctgt tgcggtgaga caccgccttt tgccgcacgc ttgtcgaggc
     2701 acgattgcag cgacagaatc ggatagacat cttcgccaat caccaggctg aatttctgca
     2761 actcactgag tggcagatct tccagcggtt tgccctgacg aatggcttcc accaccgctt
     2821 cacccacaat atggtgcgcc tcgcggaacg gtacgccttt cgccaccaga taatccgcca
     2881 gttcagtagc gttggagtaa ccctgctgcg ccgcttcctg gcaacgcgga cgtttcacct
     2941 gaatgccgtc cagcaccagc gccgccatat gcaggcagtc cagccaggta tcgagcgcgt
     3001 cgaacagacc ttctttgtct tcctgcatat ctttgttgta agccagcggc aaacctttca
     3061 gcgtcatcat catgccggtt aacgccccct gcacccgacc gcatttaccg cgaatcagct
     3121 ccagcgcatc cgggtttttc ttctgcggca ttaatgatga accggaagtc acacggtcag
     3181 aaagctccac aaaccccgct tcgccggtgt taaagaaaat cagatcttca gcaaaacgcg
     3241 acagatgcac catgccgata gcggcagcag aaagcagttc caacacatgg tcacggtcag
     3301 aaacgctgtc gagactgtta cgggtcgccg aagcaaagcc cagccaacct gctaactgtt
     3361 cacggtcgat ttcataggcc gttcctgcca gtgcgccaca gcctagcggg ctgacatcca
     3421 gacgcttaag cgcatcctgc aaacggcttt catcacgcgc cagcatctca acgtaggcca
     3481 ggcaccagtg cgcgaacgtc accggctggg cgcgttgcag gtgggtgtaa cccggcatca
     3541 ccgcgtcctg attgttctgc gcggtctcga ccagagcgga ttgtaactgc cggttagccg
     3601 tcagcaactc gctaacggta tctttgcacc acagtttcag gtcagttgct acctgatcat
     3661 tacggctacg cccggtatgc aattttttgc ctaactgacc cactttgtcg atcagtttgc
     3721 cttccaccca gctatggata tcttcggcgt cgctttcaag gatttgttgt ggcctggcgc
     3781 gaacatcttc cagcagcacg ttcagcgcct cttccagttg cgcctgctct tctgcggtta
     3841 acacgccgac cgttaccagg gctttggacc aggccacaga gccaacaata tcctgctccg
     3901 ccagacggta atcaaagcgc agtgagtcgt tgaattgttt gaaccgttga tctgctgcct
     3961 gggtaaaacg cccgccccaa agtgccataa ctctgtttcc ttattttgaa attcaatgcc
     4021 ggaaagctgc gcctccggcc aacaaaactt aagctaaaat ccgcgtcccc atcggcatac
     4081 cgttaaacag tgccggaagc tgttccgcat gacgccagga ggcgatatct accggacggc
     4141 ccaacgtgcg cgcagcatcc agcgccgcgt tcactttcac aatcatgccg tcagtaataa
     4201 tgccctgctc aatcagttgt tctgctttcg cggcggtcat ttcggcaata cgttgccctt
     4261 tgccgtcgag aatgccgctg acgtcagaga gcaaaatcag atccgcgccc agcgttgccg
     4321 ccagcgccgt cgccgcctgg tcggcattga cgttcatcag ttgcccttcg tctgttacgc
     4381 caatggagct gaccaccggc agataaccgt tctccagcaa actgttgata agcttaggcg
     4441 aacctggctg cgccagtcca acatggccta actcttcatc aagctgggtc actttgatgc
     4501 tgtcgccgtc accgagaaac aaacctacgg ccgcaatctg atgtttcttc gcccatgcca
     4561 gcagggtttt atttgccgtt cccgccagtg ctccggtgat aatgtctatc tgatccgcag
     4621 gcgtcacccg caggccattt ttctttttca ccggcagatt cagccctttc atcagctcat
     4681 ccaccacaca accgccgccg tgcacaatca ccagcggacg ctgatgtgac tcgcgataat
     4741 tcaccagtgc gctaaacaga cgttccagcg cctcttcact atccagcagt acgccaccca
     4801 gtttgataat taatggattc atcattgcac ccttaaataa gagactgcgt ttccgcatag
     4861 ccgaaacgaa tattggcgca ctgcaccgct tgtgccgccg cgcctttcag taagttgtct
     4921 tcggtcgcca caataatcag atgctcgccc tgaacggcaa aaccgatatc gcagaatggc
     4981 agtccaacga catttttcag cgccggaacg cctttgtcat acagccgcac cagtggttta
     5041 tgggcatacg cctgctgtaa cgcttgcgcc acttgcacct ggctcacacc cggtttcagg
     5101 cggcaggtaa tggtttcgag aatgccgcgc gggaaattgc ccagatgcgg ggtgaagata
     5161 acgtcagcac cgaggtgtgt ggcgatctct ggttgatggc gatgggtaaa gacgccatac
     5221 ggttgcaggc taacttcaca aaaactattt gaaatcgctg ccttacgtcc tgcaccgctc
     5281 acgccgctgg tggcgttgat caccggccac tgattgaggt caagaagatc ggcatcaatc
     5341 aacggtttca gcgccagctg tgctgccgtc ggataacagc ctggcacggc aatcaagttc
     5401 gcttctttta atttattacc gcaccactcc gccagaccgt aggctgcctg ttccagcagt
     5461 tccgggtatt gatgggtaaa gccgtaatat ttttcataga aggcgacatc gttaacacga
     5521 aacgcgccgg aaaggtcgaa caccacacag cctgcttcaa gaaattgcgg cgctaaatcg
     5581 tggctgactt catgggcggt ggcgagaaac accacgtcca cccctgggct aaactcgccg
     5641 atatccgaca tcggctgcaa cggcagatca acgatgcctt ttagctgcgg atgcaaatcg
     5701 gagattaact ttcccgcatc attgctttgc gctgaaacag tcaaagcggt tatgttcata
     5761 tgcggatggc gatttacata ggtcactagc tctgcgccag cgtagccact ggcacccaca
     5821 atcagcgtat tcaacatcgg ggctattcac cttcttatgt ctggttgcca ggttaaacgt
     5881 aaaacattca ccttacggct ggtgggtttt attacgctca acgttagtgt atttttattc
     5941 ataaatactg catgaatatt gatactatca tgaccagagg tgtgtcaaca atgaaaaaca
     6001 aattaccgcc atttatcgag atttaccgtg ctctgattgc cacaccttca ataagcgcca
     6061 cggaagaggc actcgatcaa agcaatgcag atttaatcac tctgctggcg gactggttta
     6121 aggatttagg cttcaatgtg gaagtgcagt ctgtgccagg aactcgcaac aaatttaata
     6181 tgctggccag ttgcggacag ggggctggcg gtttgttgct ggcggggcat accgatacgg
     6241 tgccatttga tgacggacgc tggacgcgcg atccatttac gttgacggag catgacggca
     6301 agctttacgg cttaggcacc gccgacatga aaggcttttt tgcgtttatc cttgatgcgc
     6361 tacgcgatgt cgacgtcacg aagcttgcaa aaccgctcta cattctggcg actgccgatg
     6421 aagaaaccag tatggccgga gcgcgttatt ttgccgaaac taccgccctg cgcccggatt
     6481 gcgccatcat tggcgagcca acgtcgctgc aaccggtacg cgcacataaa ggccacatct
     6541 caaatgccat tcgaattatg ggtcagtcgg gccactccag cgatccgtcg cgcggggtta
     6601 acgctatcga actgatgcac gacgccatcg gccatatttt gcaattgcgc gataacctga
     6661 aagaacgcta tcactatgaa gcgtttaccg tgccataccc cacactcaac ctcgggcata
     6721 ttcacggcgg cgacgcctct aaccgtattt gcgcttgttg tgagttgcat atggatattc
     6781 gtccgctgcc aggcatgaca ctcaatgaac ttaatggttt gctcaacgat gcattggctc
     6841 cagtgagcga acgctggccg ggtcgtctga cggtcgacga gctgcacccg ccgatccctg
     6901 gctatgaatg cccaccgaat cataaactgg ttgaagtggt tgagaaattg ctcggagcaa
     6961 agaccgaagt ggtgaattac tgtaccgaag cgccgtttat tcaaacgtta tgcccgacgc
     7021 tggttctggg gcctggctca attaatcagg ctcatcagcc tgatgaatat ctggagacga
     7081 ggtttatcaa gcccacccgc gaactgatca cccaggtgat tcaccatttt tgctggcatt
     7141 aaaacgtagg ccaaacaatg tcggatgcga tgcttgcgca tcttatccga cctacacctt
     7201 tggtgttact cgaggcgatt ttttaacatt cccataagtt acgcttattt aaagcgtcgt
     7261 gaatttaatg gcgtaaattc ctgctattta ttcgtttgct gaagcgattt cgcagcattt
     7321 gacgtcaccg cttttacgtg gctttataaa agacgacgaa aagcaaagcc cgagcatatt
     7381 cgcgccaatg cgacgtgaag gatacagggc tatcaaacga taagatgggg tgtctggggt
     7441 aatatgaacg aacaatattc cgcattgcgt agtaatgtca gtatgctcgg caaagtgctg
     7501 ggagaaacca tcaaggatgc gttgggagaa cacattcttg aacgcgtaga aactatccgt
     7561 aagttgtcca aatcttcacg cgctggcaat gatgctaacc gccaggagtt gctcaccacc
     7621 ttacaaaatt tgtcgaacga cgagctgctg cccgttgcgc gtgcgtttag tcagtttctg
     7681 aacctggcca acaccgctga gcaataccac agcatttcgc cgaaaggcga agctgccagc
     7741 aacccggaag tgatcgcccg caccctgcgt aaactgaaaa accagccgga actgagcgaa
     7801 gacaccatca aaaaagcagt ggaatcgctg tcgctggaac tggtcctcac ggctcaccca
     7861 accgaaatta cccgtcgtac actgatccac aaaatggtgg aagtgaacgc ctgtttaaaa
     7921 cagctcgata acaaagatat cgccgactac gaacacaacc agctgatgcg ccgcctgcgc
     7981 cagttgatcg cccagtcatg gcataccgat gaaatccgta aactgcgccc aagcccggta
     8041 gatgaagcca aatggggctt tgccgtagta gaaaacagcc tgtggcaagg cgtaccgaat
     8101 tacctgcgcg aactgaacga acaactggaa gagaacctcg gctacaaact gcccgtcgaa
     8161 tttgttccgg tacgttttac ctcgtggatg ggcggtgacc gcgacggcaa tccgaacgtc
     8221 actgccgata tcacccgcca cgtcctgcta ctcagccgct ggaaagccac cgatttgttc
     8281 ctgaaagata ttcaggttct ggtttctgaa ctgtcgatgg ttgaagccac ccctgaactg
     8341 ctggcactgg ttggcgaaga gggtgccgca gaaccgtatc gctatctgat gaaaaacctg
     8401 cgttctcgcc tgatggcgac acaggcatgg ctggaagcgc gcctgaaagg cgaagaactg
     8461 ccaaaaccag aaggtctgct gacacaaaac gaagaactgt gggaaccgct ctacgcttgc
     8521 taccagtcac ttcaggcgtg tggcatgggt attatcgcca atggcgatct gctcgacacc
     8581 ctgcgccgcg tgaaatgttt cggcgtaccg ctggtgcgta ttgatatccg tcaggagagc
     8641 acccgccata ccgaagcgct gggcgagctg acccgctacc tcggtatcgg cgactacgag
     8701 agctggtcag aagccgacaa acaggcgttc ctgatccgcg aactgaactc caaacgtccg
     8761 cttctaccgc gcaactggca accaagcgcc gaaacgcgcg aagtgctcga tacctgccag
     8821 gtgattgccg aagcgccgca aggctccatt gccgcctacg tgatctcgat ggcgaaaacg
     8881 ccgtccgacg tgctggctgt ccacctgctg ctgaaagaag cgggtatcgg gtttgcgatg
     8941 ccggttgctc cgctgtttga aaccctcgat gacctgaaca acgccaacga tgtcatgacc
     9001 cagctgctga atatcgactg gtatcgcggc ctgattcagg gcaaacagat ggtgatgatt
     9061 ggctattccg actcagcaaa agatgcgggc gtgatggcag cttcctgggc gcaatatcag
     9121 gcacaggatg cattaatcaa aacctgcgaa aaagcgggta ttgagctgac gttgttccac
     9181 ggtcgcggcg gttccattgg tcgcggcggc gcacctgccc atgcggcgct gctgtcacaa
     9241 ccgccaggaa gcctgaaagg tggcctgcgc gtgaccgaac agggcgagat gatccgcttt
     9301 aaatatggtc tgccagaaat caccgtcagc agcctgtcgc tgtacaccgg ggcgattctg
     9361 gaagccaacc tgctgccacc gcctgagccg aaagagagct ggcgtcgcat tatggatgaa
     9421 ctgtcagtca tctcctgcga tctctaccgt ggctacgtac gtgaaaacaa agattttgtg
     9481 ccttacttcc gctccgctac gccggaacaa gaattgggta aactgccgtt gggttcacgt
     9541 ccggcgaaac gtcgcccgac cggcggcgtc gagtcgctgc gcgccattcc gtggattttt
     9601 gcctggacgc aaaaccgcct gatgctcccc gcctggctgg gtgcaggtac ggcgctgcaa
     9661 aaagtggtcg aagatggcaa acagagcgag ctggaagcca tgtgccgcga ttggccattc
     9721 ttctcgacgc gtctcggcat gctggagatg gtcttcgcca aagcagacct gtggctggca
     9781 gaatactatg accaacgcct ggtcgacaaa gcactgtggc cgttaggtaa agagttacgc
     9841 aatctgcaag aagaagacat caaagtggtg ctggcgattg ccaacgattc tcacctgatg
     9901 gccgatctgc cgtggattgc agagtctatt cagctacgga atatttacac cgacccgctg
     9961 aacgtattgc aggccgagtt gctgcaccgc tcccgccagg cagaaaaaga aggccatgaa
    10021 ccggatcctc gcgtcgagca ggcgttaatg gtcactattg ccgggattgc ggcaggtatg
    10081 cgtaataccg gctaatcttc ctctcctgca aaccctcgtg cttttgcgcg agggttttct
    10141 gaaatactgc tgttctaaca ccctcgtttt caatatattt ctgtctgcat tttattcaaa
    10201 atctggatat cccttcagat atccttaagg aattgtcgtt acattcggcg atattttttt
    10261 caagacaggt tcttactatg cattccacag aagtccaggc taaacccctt tttagctgga
    10321 aagccctggg ttgggcactg ctctactttt ggtttttctc tactctgcta caggccatta
    10381 tttacatcag tggttatagt ggcactaacg gcattcgcga ctcgctgtta ttcagttcgc
    10441 tgtggttgat cccggtattc ctctttccga agcggattaa aattattgcc gcagtgatcg
    10501 gcgtggtgct atgggcggcc tctctggcgg cgctgtgcta ttacgtcatc tacggtcagg
    10561 agttctcgca gagcgttctg tttgtgatgt tcgaaaccaa caccaacgaa gccagcgagt
    10621 atttaagcca gtatttcagc ctgaaaattg tgcttatcgc gctggcctat acggcggtgg
    10681 cagttctgct gtggacacgc ctgcgcccgg tctatattcc aaagccgtgg cgttatgttg
    10741 tctcttttgc cctactttat ggcttgattc tgcatccgat cgccatgaat acgtttatca
    10801 aaaacaagcc gtttgagaaa acgttggata acctggcatc gcgtatggag cctgccgcac
    10861 cgtggcaatt cctgaccggc tattatcagt atcgtcagca actaaactcg ctaacaaagc
    10921 tactgaatga aaataatgca ttgccgccgc tggctaattt caaagatgaa tcgggtaacg
    10981 aaccgcgcac cttagtgctg gtaattggcg aatcgaccca gcgtggacgc atgagcctgt
    11041 acggttatcc gcgtgaaacc acgccggagc tggatgcgct gcataaaacc gatccgaatc
    11101 tgaccgtgtt taataacgtg gttacgtctc gtccgtacac cattgaaatc ctgcaacagg
    11161 cgctgacctt tgccaatgaa aagaacccgg atctgtatct gacgcagccg tcgctgatga
    11221 acatgatgaa acaggcgggt tataaaacct tctggatcac caaccagcag acaatgaccg
    11281 cccgcaatac gatgctgacg gtattttcgc gccagaccga caagcagtac tacatgaacc
    11341 agcaacgtac gcagagtgcg cgtgaatacg acaccaacgt gctgaagccg ttccaggaag
    11401 tgctgaagga ccctgcgccg aagaaactga tcatcgttca tctgctgggt acgcatatca
    11461 agtacaagta ccgttatccg gaagatcagg gcaaatttga cggtaataca gagcatgttc
    11521 caccgggatt aaacgcagaa gagctggagt catataatga ttatgacaat gctaacctgt
    11581 acaacgatca cgtggttgcc agcctgatta aagactttaa agcgaccgat ccgaacggtt
    11641 tcctggttta tttctctgac cacggtgaag aggtttacga cacgccgccg cacaaaactc
    11701 aggggcgtaa tgaagacaat ccgacgcgtc atatgtacac cattccgttc ctgctgtgga
    11761 cgtcagaaaa atggcaagcg actcatccgc gtgatttctc acaggatgtt gatcgtaaat
    11821 acagcctggc ggaactgatc cacacctggt cagatttggc gggcttatct tacgacggtt
    11881 acgatccaac ccgttcagtg gtgaatccgc agttcaaaga aactacccgc tggattggta
    11941 acccgtataa gaaaaacgca ctgatcgatt acgacactct gccgtatggc gatcaggtgg
    12001 gtaatcagta attatcagcc agatgttatt tgccggatgc gacgcttgac gcgtcttatc
    12061 cggcctacag atcgcggcgg taggccggat aaggcgttta cgccgcatcc ggcaatacaa
    12121 gtcgactgaa actgccgcaa tgtttacatc tcactcccat caggccacac ttttcaggtg
    12181 gccttttttc ataatgccag ttactgtttt cccgtctaca tacctaaggg aataacatgt
    12241 atcacgacgt cagctatctg ctctcccgcc tgatcaacgg cccgctgtcc ctgcgccaga
    12301 tttactttgc cagcagcaac ggcccggtgc ccgatcttgc gtatcaggtc gattttccac
    12361 gtctggaaat tgtgctggaa ggtgagttta ttgataccgg cgctggagca gcgttagttc
    12421 ccggcgatgt gctgtacgtc cctgctggtg gctggaattt tccacaatgg caagcccccg
    12481 ccacgacctt tagcgtgcta tttggcaaac agcaactcgg cttcagcgtc gtgcaatggg
    12541 atggcaaaca atatcaaaat ctggcgaagc aacacgtcgc ccgacgcggc ccacgcattg
    12601 gttcttttct gctacaaacg ctcaatgaaa tgcaaatgca gtcgcaggag cagcaaacgg
    12661 ccagacttat cgtcgccagc ctgcttagcc actgccgcga tttgctcggc agccaaatac
    12721 agaccgcctc acgcagccag gctctattcg aagctattcg tgattatatc gacgaacgct
    12781 atgcctccgc acttacccgc gaatctgtcg cacaggcgtt ctatatttcg ccaaattacc
    12841 tctcgcacct gtttcaaaaa acgggggcca ttggttttaa cgaatacctg aatcacacgc
    12901 gactggagca cgctaagaca ttattgaaag gctatgacct gaaggtaaaa gaagtggcgc
    12961 acgcctgcgg ctttgtcgac agcaactact tctgccgtct gtttcgtaaa aataccgaac
    13021 gctcgccgtc ggaatatcgt cggcagtacc acagccaact gacagaaaaa ccgactactc
    13081 cagaataaga tgggtttgct gcggcgcaga aagcactttg cgcactgcgc tcatcaccct
    13141 ctgcggctca cgcaggaaag cgtagatgct gcattgcacg tagcgacaat gttcaaatcg
    13201 ctcagcaccg gcaagctcga tatccgtaat taacagcgca acatccgccc ggcggatatc
    13261 ctcgtccgct aaacgattct ccgttcccaa cgcgccctga gtttcaatgc tgacgcccca
    13321 cttctctaac tggcacaact tttccagccg ttccgccgcc atataagtat gcgccacgcc
    13381 gcttacgcag gcggttactg ccaccagata tgccatttta acctcccacg gtaacctgaa
    13441 atccggcccg ttctgccatt tcacgcatcg ttgccacatc ggctgacgac ggcgcagcaa
    13501 cctctttcat cgaccatgtt ttccccagca ggcggtattt cggctcgccg tactgatgaa
    13561 acggtaacag atggatctgc ttgatattca gcgggatcag cacgtccagc gcctgctgca
    13621 tattctcccg gctgagcgtg aaaccaggga tcagcggtaa acgcgggatc acgttgacac
    13681 cctcactcac cagcaaacgc agattctcca gcacgcgttg caggttcatc ttcaccacat
    13741 cccgcgcctg agccgcatcc ataattttta aatcgaacaa cacttcatcg cacaatttcg
    13801 ccagcggtaa cagcttgctg gctggcgcgt ctccggcagt ttcgatggcg catgacacac
    13861 cccatagccg cagtcgctgt aaaaaacggg tcgcaaactc cgcctgcatt aacacttcgc
    13921 cgccagaaag cgtgacgccg ccgccggacg tgcgaaagaa aatgtcatct ttcatcactt
    13981 cccgttccag cgcgtcaagg ctgatatcgc ggccaatccg ttcaaatgcc ccggaggggc
    14041 attcatccgc atcacgcaaa catttcgcgc agtgcagaca tttcgcctct ctgcgcaccg
    14101 tctggatttt gccggagatc gactccggat tagcacacca tgggcaaaga tgcggacagc
    14161 ctttaaaaaa gaccaccgta cgaatgccct cgccgtcatt cagtgaataa cgctgaatgt
    14221 tgaaaatgcg cgccacatca tcgcggcgcg tttccacaac gttacagctg atgcgctgtc
    14281 cggcggatga tgtcatcctg gatctccttc gacagttcga caaagaaggc gctgtatccg
    14341 gcaacgcgca ccaccagccc ggcataatct tgtgggcgct gttgcgcttc ccgtaacgtg
    14401 tcggcgttca ccacgttaaa ctgaatatgc tgcaacttaa gctgggtaaa cgcccgtaag
    14461 aagtcggcca gtttgcgtaa tcccgcttca ccttcgaggg tcgccggagt gaatttcacg
    14521 ttcagcaatg taccgttaga aagcagtgtg ttatcgagct tactgactga tttcagtacc
    14581 gccgttggtc cttgtgcgtc ctgacccagc atgggtgaca agccaccgtc tgccagttgc
    14641 tctccggcaa agcgaccgtc tggcgtcgcg ccaaccaccg ctcccaacgg gacatgagca
    14701 gaaacggtat acgatcccgg cgtgaagtag ccgccgcgcg ggttctggta tttttccact
    14761 tctttgcagt agtggcgtaa cagttcggcg ctaatgttat ccacctcgtc gatatcgtta
    14821 ccgtatttct cgaagcggtt aattaagcga gcgcggactt tttcgccttc tggcgtggcg
    14881 aagttggctt ttaataccga cagcaattcg tcaaaactta aacgctgttg atcaaaaatc
    14941 atccctttca acgcatggag agaatcgctc aggttggcga taccgatccc ctgtacgccg
    15001 gagaagttat aacgcgcgcc gccatcggta atatcgcgac ctttttccag acaatcgctg
    15061 ataaaagacg agagtagcgg tacaggtgcc cagtcgcgat ggccgatatc acaaatattg
    15121 ctgccctcaa ccatcagggt gatgtagtgg ctgatcttgg cgcggatctg ttccagtaaa
    15181 ccttcataag tcagcgcagc gttgccttca ttttcatgca ggcagatttc catcactttc
    15241 agcaggttaa acatcgcgat gtcatgcaag ccgtaggttc tgccgggaat agataattcc
    15301 acacagccca ctacggaata gtcgcgcgca tcttccagca aaacgccacg gttgaggaac
    15361 gctggcacta ccacttcatc gttaaagatt tgcggaatac cggtgccgag gcgaatggtt
    15421 tctgcggttt tcatcaggaa cggcgtgtcg atcaaggcgt tagtgcgcac gccgaggttc
    15481 ggttgcggta attgcacgct ttgataagcg tcaaggcaaa ggaacgaaag cacgttcacc
    15541 gcgctacgtc cgttctcggt taacccgccg agcagtgcgg tatagccggt agggaaaccg
    15601 gcgaaataac gcgcgctact ggtggagcgc aacagcacga tgtcgttgca tttcacccat
    15661 aaagattcga gcagttcttt caggaatgcc ggatcttcgc cctgggttaa tgatgtctga
    15721 tagaacggca acatatactg gtcgaagcgc cccaacgata gcgaactggc gttggattcg
    15781 tattgcagaa tgatgttcat gtaccagaat aactggcacg cctgccagaa cgtctgcggc
    15841 ttatgttcgg cgttatggcg ggagatctcc gcaatagtca gcaattcttc gcgacgcggg
    15901 ccatcagtgc agcttgccgc catcgtttcc gccagttcgg cgtaacgcaa aatatgtttc
    15961 tgcgaggctt ccagcagtaa cagcgctgcc tgataaaagt gattctccgg ctgttgctga
    16021 caatgttgct gcatctgtgc caccagctcc cccagcccgt gattcagcag gcgcgggtaa
    16081 tcaataataa tgtgcccctg gcctttatcc gtctggttga tgctgaaaat ctgcgtgctg
    16141 gtcgcggctt ttacttcatc cgtcatctgc ccgttgatga aatctttcat cgaacgtttt
    16201 tcccagtacg ggaacaactc ttcgcggtag atacgtttat cttcttcgct gatagcaaag
    16261 cggtcctgcg gacgcgtcgg gaattgatcc agttctttca gcagccagta agggtccatt
    16321 tccggcgaca taatcccggc gcgtggtttt acggtgcggt taccggcaat cagttcttca
    16381 tcacgaatcg aaatttcaac gtgttcaagg atatacgctg ttgctttcgc ccgacgcatt
    16441 atcaccggct ccccttcggt ttgccgatgg ctggcggtat aaagcaacgc ccgctccagc
    16501 gagatttcac gggtattggc aaacagtgca gttttgaggc gagagatacg attcgtcatg
    16561 aggaactcct gtgggaaagc gaacccgccc ttgcgggcgg gataagaact taagcggttt
    16621 gtgccaggtg agcttcaatt ttgctcataa tggcgtcagc acgctttacc gcgtcgctga
    16681 tgttgacacg cacgatagtc ttgccagcaa aacgctcttc aaatttgatg ccgatatctt
    16741 tggtcaaaat caccatatcg gcgcttgcca cgtcttccgc agtcagttca ttttccagac
    16801 caatcgaccc ctgggtttct acttttactt cccagccttt cgctttcgcg gcgctttcca
    16861 gcgcctctgc cgccatataa gtatgggcaa cacctgaagg acatgcggtt actgcgataa
    16921 ttttcgtcat ggctcgttcc tcaattaatt aatttcaaaa tccagatcca ggtcgtcttc
    16981 cttctcttca gttgaagacc catttttacg cgccagactt ttcagcacgt tcacgcagac
    17041 tgccgtcaca accgccccga cagccactgc cgcgatgtaa cccaacttac cttcaaccac
    17101 cggcagcaca atcagaccac cccagcctgc atagcactgc gcaccgacca gcgccgcagt
    17161 taccgcacca caaactgaac cgaccatgat ggaaggaatg acacgcagcg gatcggcagc
    17221 ggcgaaagga atcgcccctt cagtaacgcc aacacagccc atcaccagtg ccgccttgcc
    17281 ggtttcgcgc tcttctgcgg agaaattttt acgaccaatc agcgtcgcca gccccatccc
    17341 cagcggtgga acacaaatcc caacagcggc aatagccacc acggtataaa caccctgagc
    17401 aacgcaaatc agcatgaagg cataggccac tttgttaacc gggccgccca tatcgaacgc
    17461 cagcatcaga cccatgatca ccgccagcat aacaatgctg ccctgctgca tcccctgaag
    17521 ccactgggtc aggctgttgg tcaacgcccc caccggctcg cccagccccc acatcatgac
    17581 gccagcggta atcagtgtgc cgacgattgg aatgatgaag attggcatta ccgagcgcag
    17641 aactttatgc actggaattt tcttcaggta atgcaccacg atgccgccga taatcccggc
    17701 gatcagcgca ccaaagaagc ccgcgccaaa gctgttacca acccaggcac cgatagcgca
    17761 tggagccagc gcagaacgtt ctgcaatgga ataaccgatg taagcggcga ggaaaggcac
    17821 catcagcgtc aaacctgcaa cgccgatatc aaacagtttt ttcagattcg gatcggctac
    17881 ggcatccggc actgcgcctt tgccatacaa catgacggaa accgccagca aaataccgcc
    17941 cgataccacg aagggaatca tgtgtgaaac gcccgtcatt aaatgctgac gggtattttt
    18001 caggatctgc accaactcat tcataagctg ctcccgggct tgcgaaagcc catgtccata
    18061 tttttgatgg aagaaacaat aggcaatcgc aggcggagca aacagtggat aaaaaggcca
    18121 tttactggac ttttgtaata tcagtacaaa aatgcgatcc gcctcataac ttgcgataat
    18181 ccggaagtac gccgcaaaac ggcgtcttgt gagacgcgtc gcaaattgac ggttacatta
    18241 catttgtcca gcttttggca gatttgtcac atggcaggcc gttgccgtgc gctcttatcc
    18301 tgtaaacaga aaaccgtacc gggagaaagg caatggccct gattgtggaa tttatttgtg
    18361 agctacctaa cggcgtccat gcgcgtccgg caagccacgt tgaaacgctg tgtaatactt
    18421 tttcatcaca aattgagtgg cataacctgc gcactgaccg caagggcaac gccaaaagcg
    18481 cccttgcgct gattggcacc gatacgctgg tgggcgataa ctgccagtta ctgatttccg
    18541 gggccgacga acaggaagcg caccagcgtt taagccaatg gctgcgcgat gaattccccc
    18601 actgcgacgc accgctggcg gaagtaaaat ctgacgaact ggaaccactg ccggtttcac
    18661 tgaccaatct gaatccgcaa attatccgcg ctcgcaccgt gtgcagcggt agcgcaggcg
    18721 gcattctgac gccgatctct tctttagatc tcaatgcgct gggtaatctt cccgcagcca
    18781 aaggcgttga cgccgaacaa tccgcactgg aaaacggtct gacgctggta ctgaaaaaca
    18841 tagagtttcg tctgctggat agcgacggtg ctaccagcgc gattctggaa gcgcaccgat
    18901 ccctggctgg cgatacttcc ctgcgcgaac atttactggc aggtgtcagc gccggattaa
    18961 gctgcgccga agcgattgtt gccagtgcga atcacttttg cgaagagttc gcccgttcca
    19021 gcagcagtta cctgcaagaa cgtgccctgg acgtacgcga cgtctgcttc cagttactcc
    19081 agcaaatcta cggtgagcaa cgcttcccgg caccgggcaa actgacgcag cccgccattt
    19141 gtatggctga tgaactgacc cccagccagt tcctcgaact ggataaaaat catctcaaag
    19201 gtctgttact gaaaagtggc ggcaccacat cacatacggt gatccttgcc cgttcgttca
    19261 acattccgac gctggtaggt gtggatattg atgcccttac tccgtggcag catcagacga
    19321 tttatatcga cggcaacgca ggggcgattg tggttgagcc aggggaatcc gtggcccgtt
    19381 attatcagca agaagcccgc gtacaggacg ctctgcgtga gcaacagcgt gtctggctga
    19441 cccagcaagc gcgtaccgct gacggtatcc gcattgaaat tgccgctaac atcgctcact
    19501 ccgtggaagc gcaggccgca ttcggcaatg gtgcggaagg cgttggtttg ttccgcactg
    19561 aaatgctcta tatggatcgc accagtgcac cgggtgaaag cgagctgtac aacatctttt
    19621 gtcaggcgct ggagtctgcc aacggacgca gcattattgt gcgcactatg gacattggtg
    19681 gtgacaaacc cgttgattat ctgaacattc ccgcagaagc taacccgttc ctcggttatc
    19741 gcgcggtgcg tatttatgaa gaatacgcgt cgttgtttac cacacaacta cgatcgatcc
    19801 tgcgcgcctc tgctcacggc agcctgaaaa tcatgatccc gatgatctcc tcaatggaag
    19861 agatcttatg ggtgaaagaa aaactggcgg aagccaaaca gcaactgcgt aacgaacaca
    19921 ttccgtttga tgagaagatc cagctcggca tcatgctgga agtgccgtcg gtgatgttca
    19981 tcatcgatca atgctgcgaa gagattgatt tctttagtat tggtagtaat gacctgacgc
    20041 aatatctact ggcagtggat cgcgataacg ctaaggtgac tcgtcactac aacagcctga
    20101 atccggcctt cttgcgggcg ctcgattacg ccgtgcaggc ggtgcatcgc cagggcaaat
    20161 ggattggtct gtgcggtgaa ctgggagcga aaggttccgt gctgccgttg ctggtcggtt
    20221 tagggctgga tgaactcagc atgagcgcac catcaattcc ggcggcgaaa gcgcggatgg
    20281 cgcaacttga tagccgtgag tgccgccagt tgctcaacca ggcaatggcc tgccgtactt
    20341 cgctggaagt ggaacacctg ctggcgcaat tccgcatgac ccaacaggac gcaccgctgg
    20401 tcaccgccga gtgcatcaca ctggaaagcg actggcgcag caaagaagaa gtgctcaaag
    20461 gcatgaccga taacctgctg ctggcgggcc gctgccgcta tccgcgtaaa ctggaagccg
    20521 acttgtgggc gcgcgaggcc gttttctcta ccggactggg ctttagtttc gccattcccc
    20581 acagtaaatc ggaacacatt gagcaatcca ccatcagcgt agcgcgtctg caagcgccag
    20641 tgcgctgggg cgatgatgaa gcgcaattca tcattatgtt aaccctgaac aaacacgccg
    20701 cgggcgatca acatatgcgc attttctcgc gcctcgctcg tcgcatcatg cacgaagaat
    20761 tccgtaatgc gctggttaac gccgcctctg ccgacgctat cgccagcctg ctgcaacatg
    20821 aactggaact gtaaaaggaa acatcatgga actgtatctg gacaccgcta acgtcgcaga
    20881 agtcgaacgt ctggcacgca tattcccgat tgccggggtg acaactaacc cgagcattat
    20941 cgctgccagc aaggagtcca tctgggaagt gctgccgcgc ctgcaaaaag cgatcggtga
    21001 tgagggcatt ctgtttgctc agaccatgag ccgcgacgcg cagggtatgg tgaaagaagc
    21061 gaaacgcctg cgcgacgcta ttccgggcat tgtggtgaaa attccggtaa cctctgaagg
    21121 tctggcagca attaaaatgc tgaagaaaga aggcattact acgctgggaa ccgcagtgta
    21181 cagcgccgcg caaggattac tggcggcgct ggctggagcc aaatacgttg ctccatacgt
    21241 taaccgcgta gatgcccagg gcggtgacgg cattcgtact gtacaggagt tgcaagcgtt
    21301 actggaaatg catgcgccag aaagcatggt gctggctgcc agctttaaaa caccacgtca
    21361 ggcgctggat tgtttgctgg caggatgtga atccatcaca ctgcccttag atgtagcgca
    21421 acaaatgctt aacacccctg cggtagagtc agctatagag aagttcgagc acgactggaa
    21481 tgccgcattt ggcactactc atctctaaag gagtaattat ggaccgcatt attcaatcac
    21541 caggtaaata catccagggc gcagatgtaa ttaatcgtct gggcgagtac ctgaagccac
    21601 tggcggaacg ctggttagtg gtgggtgaca aatttgtttt aggttttgct caatccactg
    21661 tcgagaaaag ctttaaagat gctggactgg tagtagaaat tgcgccgttt ggcggtgaat
    21721 gttcgcaaaa tgagatcgac cgtctgcgtg gcatcgcgga gactgcgcag tgtggcgcaa
    21781 ttctcggtat tggtggcgga aaaaccctcg atactgccaa agcactggca catttcatgg
    21841 gtgttccggt agcgatcgca ccgactatcg cttctaccga cgcaccgtgc agcgcattgt
    21901 ctgttatcta caccgatgag ggtgagtttg accgctatct gctgttgcca aataacccga
    21961 atatggtcat tgtcgacacc aaaatcgtcg ctggcgcgcc cgcgcgtctg ttagcggcgg
    22021 gtattggcga tgcgctggca acctggtttg aagcgcgtgc ctgttcccgt agcggcgcga
    22081 ccaccatggc gggtggcaag tgcactcagg cagcgctggc actggctgaa ctgtgctaca
    22141 acaccctgct ggaagaaggc gaaaaagcga tgcttgccgc cgaacagcat gtagtgactc
    22201 cggcgctgga gcgcgtgatt gaagcgaaca cctatttgag tggcgttggt tttgaaagcg
    22261 gcgggctggc ggcggcacac gcggtacata acggcctgac cgctatcccg gacgcgcatc
    22321 actattatca tggtgaaaaa gtggcattcg gtacgctgac gcagctggtt ctggaaaacg
    22381 cgccggtgga ggaaatcgaa accgtagctg cgcttagcca tgcggtaggt ttgccaataa
    22441 ctctcgctca actggatatt aaagaagatg tcccggcaaa aatgcgaatt gtggcagaag
    22501 cggcatgtgc agaaggtgaa accatccaca acatgcctgg cggcgcgacg ccagatcagg
    22561 tttacgccgc actgctggta gccgaccagt atggtcagcg tttcctgcaa gagtgggaat
    22621 aatctactcc aaactcccgg cttgtccggg agtttgaacg caaaattgcc tgatgcgcta
    22681 cgcttatcag gcctacgcaa tctctgcaat atagtaaatt tacgtacttt tgtagaccgg
    22741 ataaggcgtt cacgccgcat ccggcacgct gtgccagtaa tctgaaactt ccacgttgtt
    22801 cgggtgtttt tttatccgct tctggacatt ttctccacag aacaatcgtt agcccggaag
    22861 gtcgaatctg tgactcttat tttcacggac gaaacagatg aaagcttcac tggcgttatt
    22921 cagtcttctg acggtattta ccagttactc cttaaaatct cccgccgttc caccaaccgt
    22981 ggtacaaatc caggccaaca ccaatcttgc catcgcagac ggcgccagac agcaaatcgg
    23041 cagcacgcta ttttacgatc cggcgtatgt gcagcttacc tatcctggcg gtgatgtccc
    23101 gcaagaacgc ggcgtatgtt ccgatgtggt gatccgtgca ttgcgcagcc agaaggtcga
    23161 tttgcagaaa ctggtgcacg aagatatggc gaagaatttt gctgcgtacc cgcaaaaatg
    23221 gcagttaaag cgcccggaca gcaacatcga tcaccgtcgg gtgcctaatc tggaaacctg
    23281 gtttacccgc cacgataaaa cacgccccac cagcaagaac cccagtgact atcaagcggg
    23341 cgatattgtc tcctggcgat tagataacgg gctggcacat attggggtgg tgtcagatgg
    23401 cttcgcccgc gacggtacgc cgctggtgat acataacatt ggcgcaggcg cgcaggaaga
    23461 agatgtactg tttagctggc ggatggtggg gcactatcgg tattttgcta aataagagat
    23521 ctccccacat gaagaaaaaa ttcagatcct ttttacactg acggcttctt ttttcttacg
    23581 gctcaccagc gccagtgcca gtacaatcag cacaataccg ctaccttcaa ctgcccccgg
    23641 attctcaccg agcagccacc aggaaaagag tacgccgcaa acgggaacgg ccagtgtgct
    23701 taagctggca atactggcag gcaagttttt caatacaaac aaccataagc tccacgccag
    23761 cgctgtcgcc agaatcgcac tgtaggccag cgcccagaac acggtgggtt gccagtcaat
    23821 ttcacgttgc ggcaccagta aagcgaccac gctcatcacc agcgccgcat acagcatctg
    23881 ccaggatgtt agcgacaata aatccacgcg cggatggcgg gcatacagac gtttagcaac
    23941 aatcgcgctc gccccccaac tcacaccgga gaggattgcc agcatggcac ttttcatcga
    24001 agagaaatcg agttgccacg gctgcaacac caaaaataag ccgaaagcgg caatcagtat
    24061 cgcgaaatat tgcccacgtc gcaggcgttc accgagaaac aacgcggcga aaatcaccac
    24121 ccagaacggc atggtatagc tcaggattgc caccttcccc gctccgccgc tgaccagcgc
    24181 ccactgtgcc aggccaacca tcccgcaggt ttgtaacagg gcaatggcta aggtgtattt
    24241 aaacggcgtc gggcgcattc cgcgaccacg taataaaaga acgatgaata aaacgagagc
    24301 gccgaaaatg cagcgtaagg cggtgaagtc gaaggcaccg atgtagctgg tgacttgctt
    24361 catgaaaatc cagctataac tccagataag tgtgagcaca accaggccac tgattgccag
    24421 tgggttgctc tttcctgcgg cagacatgag agatccagtg tgtagttttc ctgtggacac
    24481 tatacgccgg taaagaaact tcagcgactg catgaaaggc agtcgctgaa gaaggtcaga
    24541 ttacagcagg tcgaaacggt cgaggttcat cactttcacc cacgctgcca cgaagtcttt
    24601 aacaaacttc tcgtgggcat cgctgctggc gtagacttcc gccaccgcac gcaggacaga
    24661 gttagaacca aagaccagat ccgcacggct ggctgtgtat ttcacttcgc cagtttcgcg
    24721 gtcacggcct tcgaacagtt ctttcgattc gtcggtcgct ttccactcgt aacgcatatc
    24781 cagcaagttc acgaagaagt cattgctcaa tacaccaacg cggtcagtga agacgccgtt
    24841 tttgctgcca tcgaagttgg cacccagtac gcgcatcccg cccaccagcg cagtcatttc
    24901 cggcgcggtc agcgtcagtt gctgtgcttt atcaatcaac agtgactcgg tggtggaaac
    24961 gtccagacga gcgcgatagt tacggaaacc gtcagcaatt ggttccagca gttcgaacat
    25021 ctcaatgtca gtctgatcct gacgcgcatc aacgcgaccc ggcgcaaacg gtacatgaat
    25081 gctcaaacct gcggcgcttg cggctttctc aacgccaacc acacctgcca gcacgatgat
    25141 atccgccagc gaagctttgc cagactcttt ctgaattttc tccagaactg gcagagcacg
    25201 aacggctgcg gcgttcacat cccagtcacg ctgcggcatt aatgccagac gcgcaccgtt
    25261 ggcaccacca cgtttgtcgc caccacggaa ggtagaagca gacgcccagg ccaccgatac
    25321 cagctcacta acagacagac cagaatccgc aatcgcgaat ttcagatcga taatgtcctg
    25381 ctcggtcggg ttgtagatcg gctgcggcag cggatcttgc cagatcagat cttctttcgg
    25441 tacttccggc ccgatgtaac gagatttcgg ccccatatcc ctgtgcgtca gtttgaacca
    25501 ggcacgagca aaggcttcgt tgaacgcctg cggatcgttg aggaaacgac gagagatctt
    25561 ctcgaactca ggatcaaaac gcagcgtcag gtcggtcacc agcatcgtcg gtttacgttt
    25621 cttcgacgga tcaaacggat ccgggataat ttccggtgcg tctaccgctt caaactggat
    25681 tgcgccagcc gggctgcggg tctgtaccca ctcatacttg aacaggttct cgaagaaata
    25741 gttgctccac tgggtcggcg tctgggtcca gaccacttcc agaccagagg taatggcatc
    25801 tgcgccaacg ccgctgccgt aagtgctcgc ccagcctaaa ccttgttctt caatcggtgc
    25861 agcttctgga tcaggaccta catttgatgt cggaccggca ccgtgggttt tacccagcgt
    25921 atgaccaccc gcaatcagcg ccacggtttc ttcgtcattc atgcccatgt tgccgaaggt
    25981 cgcgcggata gctgctgctg ccgaaagtgg ttcgccgctg tgatccgggc cttccgggtt
    26041 aacgtaaatc aggcccatct cggttgcacc cagcggtgct ttcgccagcg cttccggatg
    26101 acggtgagtc agccaggctt tttcatcacc ccagttaaca tccagatccg gttcccagac
    26161 gtcttcacga ccggcaccaa aaccgaaggt acggaagccg gagttttcca gcgccacgtt
    26221 acccgcgagg ataaacaggt cggcccagga gattttctga ccatatttct gtttgattgg
    26281 ccacaacagg cgacgcgctt tatcgaggct tacgttatcc ggccaggagt tcagcggtgc
    26341 aaagcgttgc tgaccacgac ccgcgccgcc gcgaccgtcg attgaacggt aagtacccgc
    26401 gccgtgccag gccatacgaa taaacagacc agcgtagctg ccccagtcgg caggccacca
    26461 cggctgagat tctgtcagca gggctttcag atcttttttc aggccgtagt aatctaattt
    26521 gctgaattct ttgcggtagt caaagtcctc acccagcggg ttagaacgat tagaatgttg
    26581 gtttaacagg tcaacacgga gttgatttgg ccaccagtcg cgcgtggttg tgcccccacc
    26641 tgcgctctgg tcgtgaccgc cctgatggaa cgggcatttg ccggtggctg tcgtgttatg
    26701 gatatcgtct gacgtgctca tcaatgtgct cccctctaca gtgttaccgt tacgatacac
    26761 agcgttagag agaagttata attgaattaa tccacggatg cgatagttga gatcttacat
    26821 attgttggtt aaagagatgt agatcaaatt gatcgtaatt aagacttttt gattatgtcc
    26881 gatttaggac aaatgcttta taaaaaaggg ttttagattt gtatccttca agtaatgagg
    26941 gagaaaacaa ggctgataac cttattttcc cgccctcatt tcgaggcagc attttgtgct
    27001 ctgtttaaaa tttgtgaaca ctgtgtgatt ttcacaaaag gcacaatact tataaaccag
    27061 gtcgaactcc cagtgtatgg caaatcgcat aactcatttc agcgcggtta agcgtataga
    27121 agtggaaatc tttcactcct tcacggctta aaatcttcac catatccatg gcgatattcg
    27181 cgcccaccag cttgcgggtt tcagcatcgt catccaggcc gtcgaacatt tgtgccatcc
    27241 acgccggaat acgcacgttg gtcatgtcgg caaatttctt cgcctgttta aagttcgaaa
    27301 ccggcaaaat tcccggaatg atttccacat caatgcctgc cgagacgcag cgatcgcgaa
    27361 aacgcaggta gctttcgaca tcgaagaaga actgagtaat ggcgcggttg gctccggcat
    27421 ccacttttcg tctcaggttg agtaaatcgg cctgggcact tttcgcttct ggatgtactt
    27481 ccggatacgc cgccacggag atatcgaaat ctgccacttc ttttaacagc gtcaccaggt
    27541 cagaagcata catttctggc ttaccacttc ccggcggcag gtcgccacgc agcgccacga
    27601 tatggcgaat accgttattc cagtagtcgc gtgcaatgat ccgcagctcg gcaggcgtcg
    27661 catcaatgca ggtaagatgc ggtgccgctt ccaggccggt gcgatcttta atgcctttaa
    27721 taatgctgtg cgtacggtcg cgctcgccgg agtttgcgcc ataggtcacc gatacaaact
    27781 tcggtttcag gctgctaagg cgatcgatgg agttccacag ggtctgctcc atttcactgg
    27841 tacgcggcgg gaaaaattcg aacgaaacgt taatctgccc ctggacttct gccagactct
    27901 gattcagggc atcccgctgg ctggcgtgaa aaaagctcat accttacctc atcaatcgct
    27961 tatcgttata tgttgtgttg tgcacatcta tccgtttaga cgtccagatg taaagatgaa
    28021 ggaaaagccg aagggcgtca accgaaaaat aaccaatgat ggtgaggaaa aatcagggaa
    28081 gatgaaaaaa actcatctta attagaggcg ataaaagatt tctctatagc gcaatccacc
    28141 tcgcacgttt tgactcatat tctgacaaca cagcgacacc tgggctaatt cgaaaaacaa
    28201 cgtcctgcca ccggatggtg atgttaaggc atctgattcg gcctgcgccc tcgcgtaggc
    28261 cgaatacagg atctgcgcaa tcaacgggta acaaacagtt gagtcaattc agtgcgcact
    28321 tttatttgat atccttcacg cgcataccgt tgagctgttc atccgtaatg gtgttgcccg
    28381 ctttgaagta atcaaccacc gcgtgataaa cgtaataacc gcccgttgtg ttacgcgctt
    28441 tcccttcggt aaaggcggta aaaccatctc caccatcagc aaggaaactc tgagtagcaa
    28501 tgtgataaac cgtcgcatct tcaatgggtt tgccattcaa agtcagtgtg attacccgct
    28561 gaccaaccgg cctgttgctg tcgtacttca tttccaggcc tttcgatacc tgtaaaacgc
    28621 cattgcttaa acttgcgccg tgttccatca gactgcgtaa ttgtttaccc atgagttcca
    28681 tcgtcaccag ttcgttgggg aacggaaacg tactgatgac gccgcccatc gtgatcgccc
    28741 ctgcggggat ctcattgcgg ataccaccag agtttgttaa cgccagttgg gtgtttttac
    28801 ccgccgctac caataaagcg tcagccgcca ggttcccgag ggaagccgat tcaccatagg
    28861 cacgttttaa ttcaaccggc gattgcgcca ccgtttgttg caccacttca tcaagctttt
    28921 tattccagcc gtcgatcacc tgtttcgttt gcggatcggg cttccactcg tcagcgtaaa
    28981 tggttttaag ctcgaagttt ttcaccgtaa aattatgcgg cttctctttg tagtcgagaa
    29041 ccagtttgcc cacatcaatc ccgccgctgt cagtggagag gatgagcgta ttgccgactt
    29101 taatcggttc cggcgtaccc acatgtgcat gtccggtgat caaaatatcc agccctttca
    29161 cctgacttgc cgtctgaata tctttatcca acgcgcgacg cacatctgtg cctcccatac
    29221 tggactggcg ggctggaaca ccttcgtgga tcagggcgac ggtcaaatca accttgcctt
    29281 tgagttcatc gatataacgt tgtagccatt taatttcatc gcgcgcttca atacccacgc
    29341 gcgtggcagc agataccgta tcattaaagg caaatactcc gtgcaaacca ataacgccta
    29401 ttttcacacc gtctttttcg atgatggtat agggcttatc ccagaatgat ttactgctgt
    29461 tctgataaaa aatattgccc tgcacaatag ggaattttgc ctggctcaac tgtaataatg
    29521 tgttgtccca gccgtggtca aattcatggt tgcctattgt gacggcatca aacggcatag
    29581 tattcataat atcgataata gccttacctt tggtcaggct gctgatatat ggcccggtaa
    29641 aatagtcgcc cgcatcaaaa aaccatgtcg ctttattttt tgctttttct tgcttaacca
    29701 gcgtagtgat atttgcccag ccgccaatat cccgtttacc gtcagcgatc cacggcactt
    29761 tataaggctc gacatgtgca tggaggtcgt tggtataaat gatggtgaca tctttagcgc
    29821 aagcccagaa tggcagtgtt aatgcgatac ccgcagcaag tattttaatt ttcataaaca
    29881 gtccctttca gtgagataaa ggattgctac attacgttct gaaagactta tttatgtgag
    29941 agtaatcaca tccgcagggg atagttgccg gacaaaatca acgaccttgt gaaatcgttg
    30001 gtgatatatg tcgactctgt ttacgattaa tgcctgtaag tcgtttggtt gccgtaattt
    30061 agggcagcca acatcgacag attatagctg gcccgactat cgactgggtt atccggcact
    30121 gcactgtcgg gcatgtggca gttacccgcc gttgtttgac gagcaacaat ttcgtgactg
    30181 gttgacagtt cacttatcta cttttgcaac agagaaaggc catttttgcc cggtatgtta
    30241 tggcacagag acgatttgtt atggtcacaa tccacagggt tcgcaacgca ttcaatgccg
    30301 taactgcaaa aaagtatgga cacccaaaca atatcagaaa gaaataacga ctcccgaaat
    30361 aatcgaaacg gttgctttgc tcgtgccatt tcagggagtc agcagcgggc agaaactgta
    30421 tgttttaatc agttttgatg ctcttcgcgg taatattctt catctttcta ctaattacac
    30481 gcagcatcag gctggagaaa gtctgcatta tcgatacaga ggaaatgcag aaccagagtt
    30541 acacgacaat aatattgtac aacgcgttga tatgcgcgaa gcacaatttc tacgccgcag
    30601 tcagtttgat gaaattcagt atggcagcgc agcgctcaag cgcaatgcca aaggcgctat
    30661 tctgcgtccg gttatcactg cacacgggca tttcagagta ctgaatattt tattccctac
    30721 agtaaaaaca catgtaattt cacacgagtg ttttttaaga ggtgccatta ttacggcctg
    30781 ggctgatctg tttcgccaac agcaaggtga aatttggttt attgaagagg aaatagctga
    30841 cgataccgat aatatgccct ggcgttttca gggaaaaact tatcacggct ggtggaaaaa
    30901 tcagtggcaa ctctgggttc agggaaaaaa tcgtaaaatg gtatgtgcat taacgggcgg
    30961 aaagagtagc aaagcagaaa tgctctcttt agcaaccagc agacatttta tcgactggct
    31021 acataaacaa gcagtattta ctcacagcgc cccgctttcc gcagggcgcg ttacgcaaat
    31081 actgctttct ctggctcagg attacaacaa ctgcgccagt cggttaatat ccgactgaat
    31141 cgccccggcg gtgacgtcgc gccctgcacc tggcccgcgg atcaccagcg ggttgtcgcg
    31201 ataccagcgg ctttcgatgg caaagacgtt atcgcatggc agtagtgatg ccagcggatg
    31261 gtcttcacgc accgcttcca cgcccacacg cgctttgcca ttggcatcga aacgcgccac
    31321 gtagcgcagc accagcccca tttcgcgggc cgcttccagt cgttgcacca tctgctcgtt
    31381 aagttcatcg ccattttcaa agaaatggtc gatgctgccg ccttcgcaat gggcaggcac
    31441 cagtgattca acacgtacct ggtccggttc aatgttgtaa cccgcttcac gcgccagaat
    31501 caccagcttg cgcattacgt ctttgccgga aaggtcatca cgcgggtcag gttcggttaa
    31561 gccttgctgc cacgcctgat ccaccagttc ggtaaacggc acgctaccgt cgaattgcag
    31621 gaacagccag gagagcgtgc cggagaagat cccactgatc gacaaaatgg tatcgccgct
    31681 gtcgatcaga tcgcgcacgg tatggttgat cggcaaaccc gcaccgacgg tggcgttgta
    31741 cagccagtga cgcccggttt tttcgaaggc gtcgtggatc tggcgatatt tattgctgtc
    31801 gcttgctcct gccagtttgt tggcactgat gacgtggaaa ccgtggctgg cgaaatcaag
    31861 atactggtca gcgagctgct ggctggcggt aacgtccagc accactaaat catcatacgg
    31921 atgggcgcgc atccacagga acagcgactc ttcatcctgc tcgaccgctt catcgttgaa
    31981 gaaggctaac gcgcggctgg cgtccagacc gtcatagctc aacaggctac ggcggctgtc
    32041 caccacgcct gccagcacaa attcaaagcc ggtacgtgcc gaaagcgtac tctgctcacg
    32101 ggcgaacagt tccagccagc gggaaccgat attgcccttg ccgaacaata ccaggccgat
    32161 gcgtttttcc gcgcggaaga cggactgatg cagcccctga atcaggcttt cggtcgggcc
    32221 ggtgcgcagt actgccacca ggctgatgcc gtcatcggac tgccaggtaa attcgaccgg
    32281 ctggcctttc agttgctgcc agaagcggtg gcaatgcagc gggttacggg tgacgcccgc
    32341 cccaaccatc gccaccagcg ccagcccctg acgcaggcgc agttcgccag gtaatcccgc
    32401 ttcgtcgagg attttcagcg cactgtcggc cacttctgag gtgtagcaaa attgcagcaa
    32461 ctggcgatcg ttatgcacgc caaccgccag tggacgtacc tgagcacgtt tcaggatctg
    32521 gtcgatctct ttatgcgcca gtttgaaatc ctggctggcg ggcacctgaa actcaatcaa
    32581 gcagacatca tcatggctgg tgacaatacg cgcaccagta ccggaggcca gcacgcgttc
    32641 aatgcgcgtg gaaccttgat ccggcgtgta gctacagcgc agttgcaggt cgatttcgct
    32701 gccagaaacg ggctgtaaag tacgggcatg aagaacggga gccgccagac gcgccagttc
    32761 gctggcttca tccagacgca gcaacggcag caggcaggca tctttcactt tacgcgggtc
    32821 ggcgctatat accccggcga cgtcgctcca gatggttacg cgagaaacac ccgccagcgc
    32881 accgatttgt gtcgcggaat agtcggaacc gttacgcccc agcagcaccg tttcaccggc
    32941 gttgttacgg ctgataaacc cggtcaccac cagacgtttg cccggatgtt gtaccagtag
    33001 ctgttgcagc aacgggtaag aaagcccttc atcaacctgc ggttgcgcag cgcgttcagc
    33061 gcgtaaaaac tcgcgggcat caagccaggc cgatggcagc ccttgttgat taagcaccgc
    33121 agacatcagg cgcgccgacc acacttcacc gtggcccacc acttccgcat acaccgcatc
    33181 gttaataccg ctgtcgagca acgccgccag gcgctcaaga tcgctgacaa aagcgctaat
    33241 gaggttatcg gcttcttcgg cgggtagcag gccgctaatc agatcgcact gataacgacg
    33301 taatgtttgt tgaacctgat gcgcagagag acgatcggtc tggcttagtt tcaaccagtt
    33361 aatcaactgg ttagtggtgc taccggcggc ggaaaccacc atcatatcgt caggctgaga
    33421 gtactccgcc ataatgcccg cgacacgcaa ataacacttc acatcagcca gactactgcc
    33481 accaaattta tgcagttgac gacctttcgc ccctgcctgc gcaatcacac tcatttttac
    33541 cccttgtttg cagcccggaa gccattttcc aggtcggcaa ttaaatcttc gccatcttca
    33601 ataccggtgg agatacgcag cagcgtctcg gagatcccgg cggcagcacg cgcttctggt
    33661 gccatgcctg catgtgtcat ggttgcggcg tgagagatta aactttccac tccccctaat
    33721 gattccgcca gcgtaaacaa cgacagcccg cccaggaaac gacgcagcgt ctgctcatcg
    33781 ccatccagtt caaaactcaa cattgcgcca aagccttttt gctggcgcgc ggcaatttca
    33841 tgcccctgat tttccggcag cgacgggtga tacagtttct tcaccaacgg ctgggtttgc
    33901 agatatttca caatcgcctg cgcgttgcgc tgcgccagct ccatacgcgg caccagcgtt
    33961 cgcaacccgc gcagcagcag atagctgtca aacgcgccgc ccgtcacgcc aatattgttt
    34021 gcccaccagg caagttcagt gacaacgtcc gggtctttag caatcactac gcctgccacc
    34081 acgtcagagt gaccgttcag atatttcgtg catgaatgca acaccagatc ggcacctaat
    34141 gccagtggat tttgtaatgc cgggcttaag aaggtgttat ccaccacgct caccgccccg
    34201 acttcccttg ccagatggca aattttcgca atatccacga cgcgtaacaa tggattactt
    34261 gggctttcta ccagtaccag tttgggtttt tctgccagcg ctgcccgtaa tgcctgttca
    34321 tcgctttgat caacaaacaa cacgcgatag caaccgcgtt tcgccaggct gtcgaacagg
    34381 cgatagctac cgccgtagca gtcgtgcggc gcaaccagca gatcgccagg tttcaaaaag
    34441 acggtcgtta ccaggtgaat cgcggacatg ccggtattag taagtacggc accggcaccg
    34501 ccttccagtt ctgccagcgc acgctgaacc acatcgcgcg ttgggttgcc gcgacgcgag
    34561 taatcatgcg cacgtggttc attaaatccg gtaaagttat aggtactgga aagatggatg
    34621 ggagggacaa cgcaaccata ctgttcgtcg tcatttaacc cgctacgcac tgcgatggtg
    34681 gcctgtttac gcgtcatgtg atgaagttcc ctgggctttg tcggtgaaat gtcaggcacc
    34741 agagtaaaca ttgtgttaat ggacgtcaat acatctggac atctaaactt ctttgcgtat
    34801 agattgagca aatcccaaat agccgttaaa attatatgca ttatcacgcc gacaggtgca
    34861 ttacacgatg tcacggtaac gcctgtacgg taaactatgc gggtttacgg tcagtaccca
    34921 cattaactgt gtggtctggt ctcaatttat tgacgaagag gattaagtat ctcatggctg
    34981 aatggagcgg cgaatatatc agcccatacg ctgagcacgg caagaagagt gaacaagtca
    35041 aaaagattac ggtttccatt cctcttaagg tgttaaaaat cctcaccgat gaacgcacgc
    35101 gtcgtcaggt gaacaacctg cgtcacgcta ccaacagcga gctgctgtgc gaagcgtttc
    35161 tgcatgcctt taccgggcaa cctttgccgg atgatgccga tctgcgtaaa gagcgcagcg
    35221 acgaaattcc ggaggcggca aaagagatca tgcgtgagat ggggattaac ccggagacgt
    35281 gggaatacta aagcggaaaa gagcggcgcg gagttaacca gccgcttttt tcatacatgg
    35341 acgtttaacg atgaaaatca ggctactgat ccccggcctg ctggtttctg tgcctgcatt
    35401 tgcctggcaa ccacaaaccg gcgacatcat ctttcagatc tctcgctcat cgcaaagtaa
    35461 agcgatccaa ctggcgaccc attccgatta cagccacacc ggtatgctgg tgatacgcaa
    35521 caaaaagccc tacgtctttg aagccgtcgg cccggtaaaa tataccccgc tgaaacagtg
    35581 gatcgcccac ggtgaagata gcaagtacat cgtgcgtcgc gttaaaggcg agctaaatgc
    35641 cgaacaacag caaaaactgg cgcaaacggc aaaacgttat ctcggtaaac cgtatgactc
    35701 cagcttctcg tggagcgacg atcgccagta ctgttcggaa gtggtgtgga aggtctacca
    35761 gaatgcgctg ggaatgcgtg tgggcgagca gcagaagctg aaagagttcg atcttagtaa
    35821 cccgctagtt caggcaaagc tcaaagaacg ctacggcaaa aatattccgc ttgaggaaac
    35881 ggtagtttca ccacaagccg ttttcgacgc gccacaactc accacggtcg ccaaagaatg
    35941 gccgctgttt tcctggtaat acgaccaaaa ttcaggcaca aaaaaagcgc cgtgcggcgc
    36001 ttttttcgga aatccggtct tatttgctgc ccgggatgtt gaaacgcttg ttgaagcggt
    36061 caacacggcc accggtagca acatcacgct gtttgccagt gaagaacggg tggcacttgc
    36121 tgcacacgtc gaggttcagg tcatgaccaa cggtggagcg gattttcatt acgttaccgc
    36181 aagagcagct agcagtaatt tcttcgtatt tcgggtgaat atcttttttc atgggaaaac
    36241 ctcggtttaa ggccgcgtcg ctcttccagc cctaacgcca gacaccacgc gatgttaaaa
    36301 gtatagcttc aatacgatca tttcgtacga aggcgcgaaa tcatacagaa attaaccagc
    36361 gtatgcaaac tgatccgcac tcttctacgg caatgtgtat actaacccac cgaatttcaa
    36421 gtcaggatga tgctatgccc gttgcccacg ttgccttgcc cgttccgctt cctcgtacct
    36481 ttgactatct gctgccagaa ggcatgacgg ttaaagccgg gagtcgcgtg cgcgtgccgt
    36541 ttggcaaaca acaggagcgc atcggggttg tggtgtcagt gagcgatgtc agtgagctgc
    36601 cgcttaacga gctgaaagcg gtagttgaag tgctggacgt tgagccggta tttactcact
    36661 ccgtctggcg attactgctc tgggcggcag attactatca tcatccgatt ggcgatgtgc
    36721 tgtttcatgc cttgccgatt ttactgcgcc aggggcggcc tgcggcaaac gcgcctatgt
    36781 ggtactggtt tgccactgaa caaggtcagg cggtggatct gaacagcctg aaacgttcgc
    36841 caaagcaaca acaggcgctg gcagcgttac ggcaaggcaa aatctggcgc gatcaggttg
    36901 ccacgctcga atttaatgat gccgcgttgc aggcactgcg caaaaaaggt ctgtgtgatt
    36961 tagcaagtga aacaccagag tttagcgact ggcgaacgaa ctatgccgtt tctggtgagc
    37021 ggttgcggtt gaataccgaa caggccaccg ccgttggcgc aattcatagc gcggcagata
    37081 ctttttctgc ctggctgctg gcgggcgtta ccggttccgg taaaacggag gtttatctca
    37141 gtgtactgga aaacgtgctc gctcagggca aacaggcgct ggtgatggtg ccggaaatcg
    37201 gcctgacacc gcaaactatc gcccgttttc gtgaacgttt taatgccccg gtggaagttc
    37261 tgcattccgg cctgaacgac agcgagcgtc tttcggcgtg gttgaaagcg aaaaatggcg
    37321 aggcggcgat tgtcatcggc acccgctccg cgctgtttac gccgtttaaa aatctcggcg
    37381 tgattgttat tgatgaagag cacgacagtt cctacaaaca gcaggaaggc tggcgctatc
    37441 atgcccgcga cctggcggtg tatcgcgcgc atagcgagca aatcccaatt attcttggct
    37501 ccgcaacgcc cgcactggaa acgttatgca acgtgcagca gaaaaaatac cgcctgctgc
    37561 gcctgacccg tcgggcgggc aatgcgcgcc cggcaattca acatgtgctg gatttaaaag
    37621 gtcagaaggt gcaggcaggt ctggctccgg cgttgatcac ccgtatgcgc cagcatttac
    37681 aggccgacaa ccaggttatc ctctttctta accgccgggg ttttgcgcct gcgctactgt
    37741 gccacgactg tggctggatt gccgaatgcc cgcgttgcga tcactactac acgctgcatc
    37801 aggcacaaca gcatttgcgc tgtcaccact gtgacagcca acgcccggtg ccgcgccagt
    37861 gcccttcctg cggttccaca cacctggtac ccgtggggct gggcaccgaa cagcttgaac
    37921 agacgctcgc gccgctgttc cccggcgtgc ccatttctcg tatcgaccgc gataccacca
    37981 gccgcaaagg ggcgctggaa cagcaactgg cggaagtgca tcgcggcggc gcgaggatct
    38041 tgattggcac gcaaatgctg gcgaaaggtc accactttcc ggatgtgacg ctggtagcgt
    38101 tactggatgt ggacggcgcg ctgttttctg ccgattttcg ctcggcagag cgtttcgctc
    38161 agctttacac ccaggtcgct ggtcgtgccg ggcgtgcggg taaacagggc gaagtggtgc
    38221 tgcaaacgca ccatccggaa catcctctgt tgcaaacgtt gctctataaa ggctacgacg
    38281 actttgccga acaggcgctg gctgagcgac ggatgatgca gctgccgcca tggaccagcc
    38341 atgtgattgt gcgtgcggaa gatcataaca atcagcacgc gccgctgttc ctgcaacagt
    38401 tgcgtaatct gatcctcgcc agcccgcttg cagacgacaa actgtgggtt ctcggtccgg
    38461 ttccggcact ggcccctaag cgtggtggcc gctggcgctg gcaaattttg ttgcagcacc
    38521 cttcccgcgt gcgattacag cacatcatca atggtacgct ggcgcttatt aatacaatac
    38581 cggattcccg taaagtgaaa tgggtgctgg atgtcgatcc catcgagggc taaaccgctc
    38641 acgatgcgag gcggatcgaa aaattcaata ttcatcacac ttttcatgaa aattctgtaa
    38701 ccgttttcac gcgctatctg ctaaaaatgt tgccgatgtg aagtaaacat ggatgtagta
    38761 cgcctgacgt gccaggcgag gagtgagtgt gaaagcgaag aagcaggaaa ctgccgcgac
    38821 catgaaagac gttgccctca aggcaaaagt ctctacagcg actgtctccc gagcattaat
    38881 gaatcccgat aaagtctccc aggccacccg taatcgggtt gaaaaagcgg cccgggaagt
    38941 gggttattta ccgcagccta tggggcgcaa cgtcaagcgt aatgaatccc gcaccattct
    39001 ggtgattgtc ccggatatct gcgacccttt ctttagcgaa attattcgcg gtatcgaagt
    39061 tacggcggca aatcacggat atctggtgct gattggcgac tgtgcgcatc aaaatcagca
    39121 ggaaaaaacc tttatcgatt tgatcatcac caagcaaatt gatggcatgt tgttgctggg
    39181 ttcaaggctg ccgtttgatg ccagcattga ggaacagcgt aatctgccgc cgatggtgat
    39241 ggcgaacgaa tttgcaccgg aactggagct gcctaccgtt catatcgaca atctgaccgc
    39301 cgcatttgat gcagtaaatt atttatatga gcaagggcat aaacggattg gctgtatagc
    39361 cggtcccgaa gagatgccgc tgtgtcacta ccgcctgcaa ggctatgttc aggcgctgcg
    39421 tcgctgcggc attatggttg atccgcaata catcgcccgt ggcgacttca ccttcgaagc
    39481 cggaagcaga gcaatgcaac agctgcttga tcttccacaa ccgcctactg ctgtcttctg
    39541 ccatagcgat gtgatggcgc tcggcgcact ttctcaggca aaacgtcagg ggctgaaagt
    39601 cccggaagac ctttccataa tcggttttga taacatcgac ctgacgcaat tttgtgatcc
    39661 gccgctgaca accatcgcgc agccgcgtta cgaaatcggt cgggaagcta tgctgttatt
    39721 gcttgatcaa atgcaggggc aacacgttgg cagtggctct cgtttaatgg actgcgaact
    39781 tatcatccgg ggatcaacac gcgcgttacc ttaaagtaaa cctttaagac tgccgtgact
    39841 ggtcaaaggc ccgccgctta agtaacatgg cgggctgacg aacgaataaa tacagcgaaa
    39901 cgatagtggc acaacgagat tatgtacgcc gcagccaacc ggcaccttcg cggcgaaaaa
    39961 agagcacctc acggaaaaag caacgaaatc tgcctgcggt ttctcccgct atggtcgcta
    40021 ttgctgccgc cgttcttgtg acctttatcg gtggtctgta cttcattacg catcacaaga
    40081 aagaagagtc cgagacgctg caaagccaga aagtgaccgg aaacggacta ccaccaaaac
    40141 cagaagaacg ctggcgctac attaaagagc tggaaagtcg ccagccgggt gtgcgtgcgc
    40201 ccacagaacc ttctgccggt ggtgaagtga aaacgccgga gcaactgaca ccagaacaac
    40261 gccagcttct tgaacaaatg caggccgata tgcgccagca gccaacgcag ctggttgaag
    40321 ttccgtggaa tgagcagacg ccggaacagc gtcagcaaac gctacagcgc cagcgtcagg
    40381 cgcagcaatt agcggagcag caacgcctgg ctcagcagtc gcgtacgact gaacaaagct
    40441 ggcagcagca gacgcgtacg tcgcaagccg cgccggtaca ggcgcaaccg cgccagtcta
    40501 aaccggcttc tactcagcag ccgtaccagg atctgctgca aacccctgcg cacacgaccg
    40561 cgcagtcgaa gccgcagcaa gctgcgccgg ttgctcgtgc cgctgacgcg ccaaaaccga
    40621 cggcggagaa aaaagacgaa cgccgctgga tggtgcagtg cggttcgttc agaggcgcgg
    40681 aacaggcaga gacggtacgt gctcagctgg cgttcgaagg ctttgactca aaaattacca
    40741 ccaacaatgg ctggaatcgt gtggtgattg gcccggtgaa aggcaaagag aacgcagaca
    40801 gcaccctcaa tcgcttgaag atggcgggtc atacaaactg cattcggctc gccgccgggg
    40861 gttgaaaccc tcaaaatccc ccccatctat aattgcatta tgccccgtac ttttgtacgg
    40921 ggtttgtact ctgtattcgt aaccaagggg tcagctcgtg acaactatag taagcgtacg
    40981 ccgtaacggc catgtggtca tcgctggtga tggtcaggcc acgttgggca ataccgtaat
    41041 gaaaggcaac gtgaaaaagg tccgccgtct gtacaacgac aaagtcatcg cgggctttgc
    41101 gggcggtact gcggatgctt ttacgctgtt cgaactgttt gaacgtaaac tggaaatgca
    41161 tcagggccat ctggtcaaag ccgccgttga gctggcgaaa gactggcgta ccgatcgcat
    41221 gctgcgcaaa cttgaagcac tgctggcagt cgcggatgaa actgcatcgc ttatcatcac
    41281 cggtaacggt gacgtggtgc agccagaaaa cgatcttatt gctatcggct ccggcggccc
    41341 ttacgcccag gctgcggcgc gcgcgctgtt agaaaacact gaacttagcg cccgtgaaat
    41401 tgctgaaaag gcgttggata ttgcaggcga catttgcatc tataccaacc atttccacac
    41461 catcgaagaa ttaagctaca aagcgtaagg atctcccatg tctgaaatga ccccacgcga
    41521 aatcgtcagc gaactggata agcacatcat cggccaggac aacgccaagc gttccgtggc
    41581 gattgctctg cgtaaccgct ggcgtcgcat gcagctcaac gaagagctgc gccatgaagt
    41641 gaccccgaaa aatatcctga tgatcggccc gaccggtgtc ggtaaaactg aaatcgcccg
    41701 tcgtctggct aagctggcga atgcgccgtt catcaaagtt gaagcgacca aattcaccga
    41761 agtgggctat gtcggtaagg aagtggattc tattattcgc gatctgaccg atgccgccgt
    41821 gaaaatggta cgcgtccagg ctatcgagaa aaaccgttat cgcgctgaag aactggcaga
    41881 agaacgtatt ctcgacgtac tgatcccacc ggctaaaaat aactggggac agaccgaaca
    41941 gcagcaggag ccgtccgctg ctcgtcaggc attccgcaaa aaactgcgtg aaggccagct
    42001 tgatgacaaa gaaattgaga tcgatcttgc cgcagcaccg atgggcgttg aaattatggc
    42061 tcctccgggc atggaagaga tgaccagcca gctgcagtcc atgttccaga acctgggcgg
    42121 tcagaagcaa aaggcgcgta agctgaaaat caaagacgcc atgaagctgc tgattgaaga
    42181 agaagcggcg aaactggtga acccggaaga gctgaagcaa gatgctatcg acgctgttga
    42241 gcagcacggg atcgtgttta tcgacgaaat cgacaaaatc tgtaagcgcg gcgagtcttc
    42301 cggtccggat gtttctcgtg aaggcgttca gcgtgacctg ctgccgctgg tagaaggttg
    42361 cactgtttcc accaaacacg ggatggtcaa aactgaccac attctgttta tcgcttctgg
    42421 cgcgttccag attgcgaaac cgtctgacct gatcccggaa ctgcaaggtc gtctgccaat
    42481 ccgcgttgag ctgcaggcac tgaccaccag cgactttgag cgtattttga ccgagccgaa
    42541 tgcctctatc accgtgcagt acaaagcact gatggcaact gagggcgtaa atatcgagtt
    42601 taccgactcc ggtattaagc gcatcgcgga agcggcatgg caggtgaacg aatctaccga
    42661 aaacatcggt gctcgtcgtt tacataccgt tctggagcgt ttaatggaag agatttccta
    42721 cgacgccagc gatttaagcg gtcaaactat cactattgac gcagattatg tgagcaaaca
    42781 tctggatgcg ttggtggcag atgaagatct gagccgtttt atcctataat cgcgttcaat
    42841 cattttcatc attgtttgat ggggctgaaa ggccccattt ttattggcgc gtattatgac
    42901 tgaacaacaa attagccgaa ctcaggcgtg gctggaaagt ttacgaccta aaaccctccc
    42961 cctcgccttt gctgcaatta tcgtcgggac ggcgctggca tggtggcaag gtcacttcga
    43021 tccactggtc gccctgctgg cattgattac cgccgggcta ttacagatcc tttctaacct
    43081 cgccaatgat tacggcgatg cggtaaaagg cagcgataaa cctgaccgca ttgggccgct
    43141 acgcggcatg caaaaagggg tcattaccca gcaagagatg aaacgggcgc tcattattac
    43201 cgtcgtgctc atctgtctct ccgggctggc actggttgca gtggcgtgcc atacgctggc
    43261 cgattttgtc ggtttcctga ttcttggcgg gttgtcgatc attgccgcta tcacctacac
    43321 cgtgggcaat cgtccttatg gttatatcgg tttgggtgat atttccgtac tggttttctt
    43381 tggctggttg agcgtcatgg ggagctggta tttacaggct catacattga ttccggcact
    43441 gatccttccg gcaaccgcat gcggcctgct ggcaacggca gtactgaaca ttaataacct
    43501 gcgtgatatc aatagcgacc gcgaaaacgg caaaaacacg ctggtggtgc gcttaggtgc
    43561 agtaaacgcg cgtcgttatc atgcctgcct gctgatgggc tcactggtgt gtctagcgct
    43621 gtttaatctc ttttcgctgc atagcctgtg gggctggctg ttcctgctgg cggcaccgtt
    43681 gctggtgaag caagctcgtt atgtgatgcg cgaaatggac ccggtggcga tgcgaccaat
    43741 gctggaacgc actgtcaaag gagcgttact gactaacctg ctgtttgttt tagggatatt
    43801 cctaagccag tgggcagcat aactgacaaa tatcaattaa caattgatga ttttgccaac
    43861 agcccacata gcgcgatata ctgaaaattc tcgcagcaac tgaatgttaa gcctatgaaa
    43921 tacgatactt ccgagctttg tgacatctat caagaagatg ttaacgtcgt ggaaccgctg
    43981 ttctccaact ttggcggacg ggcgtcgttt ggcggacaaa taatcacggt aaaatgtttc
    44041 gaggacaacg ggttgctgta cgatctgctc gaacagaatg gccgtggtcg tgttcttgtc
    44101 gtcgatggcg gaggttctgt tcgccgcgca ctggtcgatg ctgaactggc gcgtctggca
    44161 gtacaaaatg aatgggaagg cctggtcatt tacggcgcgg tgcgtcaggt agatgacctg
    44221 gaagagttgg atatcggcat ccaggcgatg gcggcaattc cggttggtgc cgctggcgaa
    44281 ggcattggcg aaagcgatgt ccgcgtcaat tttggcggtg tcaccttctt ctccggcgac
    44341 catctttatg ccgacaatac cgggattatt ctttcagaag atccgctgga tattgaatga
    44401 taaaaaaggc accgcaaggt gccttttttc tgcgttacct gttggcctac acagtaaaga
    44461 aattacgcga aagatgaagt gtaatcagac ctcttccatg cgacccagca gggcctgcag
    44521 acgttcctgc cagccgttct gctgttcttt cagatggttg ttctcacgct ccagctcttc
    44581 gcgctgatgc tgggcatttt gaacttcctg cgacagtgag ttgttttttt ctttcagctc
    44641 ttcgatttcc atctgcaaca gagtgatggt atcaatcgcc tgctgtactt ttgcttccag
    44701 tttctcaaac acttctaatg acattgtcat acctctcctg aattgcaagg cgttgatgga
    44761 taaaaatcct cgtcccgatt accggtgacg ccttaataaa tacgagcgca ctttagttag
    44821 ctccgattgt atgaagccgc gccatcgctg tccagcggca cgccttgcag attacggttt
    44881 gccacacttt tcatccttct cctggtgaca taatccacat caatcgaaaa tgttaataaa
    44941 tttgttgcgc gaatgatcta acaaacatgc atcatgtaca atcagatgga ataaatggcg
    45001 cgataacgct cattttatga cggagcacac acattttaag ttcgatattt ctcgtttttg
    45061 ctcgttaacg ataagtttac agcatgccta caagcatcgt ggaggtccgt gactttcacg
    45121 catacaacaa acattaactc ttcaggatcc gattatgagt caaacatcaa ccttgaaagg
    45181 ccagtgcatt gctgaattcc tcggtaccgg gttgttgatt tttttcggtg tgggttgcgt
    45241 tgcagcacta aaagtcgctg gtgcgtcttt tggtcagtgg gaaatcagtg tcatttgggg
    45301 actgggggtg gcaatggcca tctacctgac cgcaggggtt tccggtgcgc atcttaatcc
    45361 cgctgttacc attgcattgt ggctgtttgc ctgtttcgac aagcgcaaag ttattccttt
    45421 tatcgtttca caagttgccg gcgctttctg tgctgcggct ttagtttacg ggctttacta
    45481 caatttattt ttcgacttcg agcagactca tcacattgtt cgcggcagcg ttgaaagtgt
    45541 tgatctggct ggcactttct ctacttaccc taatcctcat atcaattttg tgcaggcttt
    45601 cgcagttgag atggtgatta ccgctattct gatggggctg atcctggcgt taacggacga
    45661 tggcaacggt gtaccacgcg gccctttggc tcccttgctg attggtctac tgattgcggt
    45721 cattggcgca tctatgggcc cattgacggg ctttgccatg aacccagcgc gtgacttcgg
    45781 tccgaaagtc tttgcctggc tggcgggctg gggcaatgtc gcctttaccg gcggcagaga
    45841 cattccttac ttcctggtgc cgctttttgg ccctatcgtt ggcgcgattg taggtgcatt
    45901 tgcctaccgc aaactgattg gtcgccattt gccttgcgat atctgtgttg tggaagaaaa
    45961 ggagaccaca actccttcag aacaaaaagc ttcgctgtaa tatgactacg ggacaattaa
    46021 acatgactga aaaaaaatat atcgttgcgc tcgaccaggg caccaccagc tcccgcgcgg
    46081 tcgtaatgga tcacgatgcc aatatcatta gcgtgtcgca gcgcgaattt gagcaaatct
    46141 acccaaaacc aggctgggta gaacacgacc caatggaaat ctgggccacc caaagctcca
    46201 cgctggtaga agtgctggcg aaagctgata tcagttccga tcaaattgca gctatcggta
    46261 ttacgaacca gcgtgaaacc actattgtct gggaaaaaga aaccggcaaa cctatctata
    46321 acgccattgt ctggcagtgc cgtcgtaccg ccgagatctg cgaacatctc aaacgcgatg
    46381 gcatggaaga gtacattcgt aacaacaccg gtctggtgat tgacccgtac ttctctggca
    46441 ccaaagtgaa gtggatcctc gaccatgtgg aaggctctcg cgagcgtgcg cgtcgtggtg
    46501 aattgctgtt tggtacggtt gatacgtggc ttatctggaa aatgactcag ggccgtgtcc
    46561 atgtgaccga ttacaccaac gcctctcgta ccatgttgtt taacatccat actctggact
    46621 gggacgacaa aatgctggag gtgctggata ttccacgcga gatgctgcca gaagtgcgtc
    46681 gttcttccga agtatacggt cagactaaca ttggcggcaa aggcggcacg cgtattccaa
    46741 tctccgggat cgccggtgac cagcaggcgg cgctgtttgg tcagttgtgc gtgaaagaag
    46801 gaatggcgaa gaacacctac ggtaccggct gctttatgct gatgaacact ggcgagaaag
    46861 cggtgaaatc agaaaacggc ctgctgacca ccatcgcctg cggcccgact ggcgaagtaa
    46921 actatgcgct ggaaggtgcg gtgtttatgg cgggcgcgtc cattcagtgg ctgcgcgatg
    46981 agatgaagtt gattaacgac gcctacgatt ccgaatattt tgccaccaaa gtgcaaaaca
    47041 ccaatggtgt gtatgtggtt ccggccttta ccgggctggg tgcgccatac tgggatccgt
    47101 atgcacgcgg ggcgattttc ggtctgactc gtggggtgaa cgctaaccac attatacgcg
    47161 cgacgctgga gtctattgct tatcagacgc gtgacgtgct ggaagcaatg caggccgact
    47221 ccggtattcg tctgcacgcg ctgcgcgtgg acggcggtgc ggtggcgaac aatttcctga
    47281 tgcagttcca gtccgatatt ctcggtacgc gcgttgagcg cccggaagtg cgcgaagtca
    47341 ccgctttggg tgcggcctat cttgctggtc tggcggttgg cttctggcag aatcttgacg
    47401 agctgcaaga gaaagcggtg attgaacgcg agttccgtcc aggcatcgaa accactgagc
    47461 gtaattaccg ttacgcaggc tggaaaaaag cggtcaaacg cgcgatggca tgggaagaac
    47521 acgacgagta ataatgattg cctgatgcga cgcttgcgcg tcttatcagg cctacgagtc
    47581 ggatacacat ccggcatcac gcagactctg aaaccgtgcg ggggcaaccc cgcacacatc
    47641 aataatcccg cccttcccct gtgctacact tcgcgccatt ccttactgct tagagtttgc
    47701 tatgagacga gaacttgcca tcgaattttc ccgcgtcacc gaatcagcgg cgctggctgg
    47761 ctacaaatgg ttaggacgcg gcgataaaaa caccgcggac ggcgcggcgg taaacgccat
    47821 gcgtattatg ctcaaccagg tcaacattga cggcaccatc gtcattggtg aaggtgaaat
    47881 cgacgaagca ccgatgctct acattggtga aaaagtcggt actggtcgcg gcgacgcggt
    47941 agatattgct gttgatccga ttgaaggcac gcgcatgacg gcgatgggcc aggctaacgc
    48001 gctggcggtg ctggcagtag gcgataaagg ctgtttcctc aacgcgccag atatgtatat
    48061 ggagaagctg atcgtcggac cgggagccaa aggcaccatt gatctgaacc tgccgctggc
    48121 ggataacctg cgcaatgttg cggcggcgct cggcaaaccg ttgagcgaac tgacggtaac
    48181 gattctggca aaaccgcgcc acgatgccgt tatcgctgaa atgcaacaac tcggcgtacg
    48241 cgtatttgct attccggacg gcgacgttgc agcctcaatt ctcacctgta tgccggacag
    48301 tgaagtcgat gtgctgtacg gcattggcgg cgcgccggaa ggcgtggttt ctgcggcggt
    48361 gatccgcgca ttagatggcg acatgaatgg tcgtctgctg gcacgtcatg acgtcaaagg
    48421 cgacaacgaa gagaatcgtc gcattggcga gcaggagctg gcacgctgca aagcgatggg
    48481 catcgaagcc ggtaaagtat tgcgtttggg cgatatggcg cgcagcgaca acgtcatctt
    48541 ctctgccacc ggtattacca aaggcgatct gctggaaggc attagccgca aaggcaatat
    48601 cgccaccacc gaaacgctgc tgatccgtgg taaatcacgc accattcgtc gcattcagtc
    48661 catccactat ctggatcgta aagacccgga aatgcaggtg cacattctct gagtgatttg
    48721 atcgattgag ccttccagtc cttcgggact ggaatttttt tgttcggaga acgaagataa
    48781 ggcaagtcaa tcaaaacagg agaaaaacat ggctgattgg gtaacaggca aagtcactaa
    48841 agtgcagaac tggaccgacg ccctgtttag tctcaccgtt cacgctcccg tgcttccgtt
    48901 taccgccggg caattcacca aacttggcct tgaaattgaa ggcgaacgcg ttcagcgtgc
    48961 ctactcttac gttaactcgc ccgacaatcc cgatctggag ttttacctgg tcaccgtccc
    49021 cgatggcaaa ttaagcccgc gactggcggc gctaaaaccg ggtgatgaag tgcaagtggt
    49081 tagtgaggcg gcaggcttct ttgtgctgga tgaagtccct gactgcgaaa cactgtggat
    49141 gctggcgact ggtacggcga ttggccctta tttatcgatt ctgcaattgg gcaaagattt
    49201 agaacgcttc aaaaacatgg tgctggttca cgccgcacga tatgccgccg acttaagcta
    49261 tctacctctg atgcaggaac tggaaaaacg ctacgagggg aaactgcgca ttcagacggt
    49321 ggtcagtcgg gaaacggctg cgggttcgct cactgggcgg ataccggcgt taattgaaag
    49381 tggtgaactg gaaagcgcgg ttggcctgcc gatgaataaa gaaaccagcc atgtgatgct
    49441 gtgcggcaat ccacagatgg tgcgcgatac acaacagttg ctgaaagaga cccggcagat
    49501 gacgaaacat ttacgtcgcc gaccgggcca tatgacagcg gagcattact ggtaagcggt
    49561 tacttgtcga taaacggcac gatcaagaga tccgcactca acttattgat catcccgcga
    49621 tatgccggca tcaaacggtt gataaatgag tggtgatgac cacagacaag gaggtcgcac
    49681 tgctcttttt gcataatttc cagcagtgtt tccggcattt ctccgcgttc aatacgcagt
    49741 tttgtcttcg gccattgaat atttttcgtc agtttataca gcttgttatc cgacttattc
    49801 ttcaacaatt gaagaatatc ttctgttgca gggaagtaga tacccggata caactcgctt
    49861 aagccatcat caatatgaat taacgtcagg tgagcgtcat tatgtctggc gagctccagg
    49921 gctttattca ccagtaaggc atcttcttca ttcccggaaa ttgccacgcc aatgtgttta
    49981 taagccatgt ttaactcctt ctaaagcaac tccatcaagc taacgaacgc agtgataact
    50041 caaaaataat catctcggcc tggcatgaga aggtgaaggc cgcatcaagc tcaacttgcc
    50101 cttccagatc tttaatctta taggccacgc tggccccttc atctgcagag gatgcagcgg
    50161 cggcaagctc tttcagtttg gtcataaagg actctgcttc ttcgcgggtg gcaaaaaagc
    50221 gcgaaaactt actggtacag ttgtcgttat caatcaccgt accgatatca atcgcacatc
    50281 ctttagtact gcatttatct acgacatctt tcatagggca cctctgtata ttcgcctctc
    50341 tgtcgattac gacttaagaa taatgcgccg ttaaaggaag aacaaacaag taatgatctt
    50401 atggattacc attttttttg atattgctta aggtgaagag ttcagaagga ttttccgggc
    50461 attgtcactt gcatcgtgga aataacaatc ccggaaaaaa atagcgataa gagatatatt
    50521 aaaggttaat tacttgattt attgtcggct ttatacttca catcctgggt atctttacca
    50581 tatttatttt cgccttgtgt gccaacaaac gcaccaagat cgataagcat catcaccaga
    50641 atcagtgttg ggataaaacg ccccaccgcc cattgccata cccccggcaa aattgcccag
    50701 ttacccgcca gcagcatcca cgccacaatc atcagaaatg cccatgcgcc ggaacgcccg
    50761 cgatcatgca agcgcttaac ggttactgcc gctgttggcc aaagcaagca cacaaggcaa
    50821 aacgccgcgg tctgaatatc gagtaaattt ttacccgcca gtgagaaaag caccagcatg
    50881 cctgcgaacc acaggcctat ccaaatccag aaatcacggc gtccaatacg ccctttaaat
    50941 gagaataacc attgctgtat ggtcatgtaa gttccttgat ggttgtcttt tccaggattc
    51001 tacccttttg acaagggcga cagatatcgt tttaatcgga gccagtcata acaaaaggta
    51061 ctgtcaatga agccagggtg tacgctgttt attctcttat gttctgcatt aaccgttaca
    51121 acaacggcgc atgcacaaac accagatacg gcaacgaccg cgccttatct gctggctgga
    51181 gcccctactt tcgatctctc catcagccag tttcgagaag actttaacag ccagaatccc
    51241 agcctgccac tgaacgaatt tcgtgccatc gacagcagtc ccgacaaagc caatctcact
    51301 cgtgcagcaa gtaaaattaa cgagaacttg tatgcttcta ctgcgctgga gcgcggtacc
    51361 ttaaaaatca aaagcattca aataacctgg ctacccatcc aggggccaga gcaaaaagcc
    51421 gcgaaagcga aagctcaggc atacatggca gcggtgatcc gcacactcac cccattaatg
    51481 accaaaactc aaagccagaa aaaactgcag tcgctactaa cggcggggaa aaacaaacgt
    51541 tattacaccg agacagaagg tgcactgcgt tatgttgtcg cggacaacgg cgaaaagggg
    51601 ctgaccttcg ctgttgaacc gattaagctg gcgctatctg aatcgcttga aggtttgaat
    51661 aaatgacaaa aagcaaagcc tttgtgccga tgaatctcta tactgtttca cagacctgct
    51721 gccctgcggg gcggccatct tcctttattc gcttataagc gtggagaatt aaaatgcgac
    51781 atcctttagt gatgggtaac tggaaactga acggcagccg ccacatggtt cacgagctgg
    51841 tttctaacct gcgtaaagag ctggcaggtg ttgctggctg tgcggttgca atcgcaccac
    51901 cggaaatgta catcgatatg gcgaagcgtg aagctgaagg cagccacatc atgctgggtg
    51961 cgcaaaacgt ggacctgaac ctgtccggcg cattcaccgg tgaaacctct gctgctatgc
    52021 tgaaagacat cggcgcacag tacatcatca tcggccactc tgaacgtcgt acttaccaca
    52081 aagaatctga cgaactgatc gcgaaaaaat tcgcggtgct gaaagagcag ggtctaactc
    52141 cggttctgtg catcggtgaa accgaagcgg aaaacgaagc gggcaaaact gaagaagttt
    52201 gtgcacgtca gatcgacgcg gtactgaaaa cccagggtgc tgcggcattc gaaggtgcgg
    52261 ttatcgctta cgaacctgta tgggcaatcg gtactggcaa atctgcaact ccggctcagg
    52321 cacaggctgt tcacaaattc atccgtgacc acattgctaa agttgacgct aacatcgctg
    52381 aacaagtgat cattcagtac ggcggctctg taaacgcgtc taacgctgca gaactgtttg
    52441 ctcagccaga catcgacggc gcgctggttg gcggtgcttc tctgaaagct gacgctttcg
    52501 ccgtgatcgt taaagctgca gaagcggcta aacaggctta agtctgacag gtgccggatt
    52561 tcatatccgg cacttacttt cctaaactcc tcgccttaac gcaaaatctc acactgatga
    52621 tcctggattt cttcggctga agcacggttc agcgtcagta gatttcgttg tgtcgccagc
    52681 aatacaaatg agttatcact ctgccgtacc atcgccagcc cgtagcttcc catatgttcc
    52741 cgcgcctcag gcacttcttc cgccagcatc ataaatgggc tgcgttgtac cagttcgctt
    52801 tccgttaccc gacgcgccag gtactcatgc ccacgcaaac cacctggcag tggcaaccag
    52861 cggctactga tgttcgccag attgttatcc agctgttcgc gcacatcagg acgaatacaa
    52921 gaaatatgaa tatgaaaatg gttttgcgta cgcccggtgc gggagttgat cgccagagat
    52981 accgcgcgat cgggaacagg ctggccgtat ttttggctca taaaatcacg cgcctgccag
    53041 gctaaccaga aaaaattagg cgttgacgga tcggtcaaca atgggctttc agtaccgtta
    53101 atacggtacg ttggcattaa cagatattgc agtgggccat gacgatcttt taaaaccacg
    53161 tatccggcgt tgggtttgac ttccgcacat ggcgacgggt tttggttttc ctgctggttg
    53221 ggcaaacatt gctcaagaac tatcttacgt aacgtgtctg actcttcacc ggttaatttc
    53281 cagtaaccaa taccggcagt cacaacggcg ataactatca tcaccaaaaa aagaagaccc
    53341 gcttttttca tctttttttc cctgtacctc aaagagatgc aagggtaacg caaaatcgtg
    53401 acaaataaaa aacccagtgg cgagaaacca ccgggcttga taattatcct ggggaaatca
    53461 gcgtttgctg atctgatcga acgtaccgcc gttagcaaaa tgctcttttt gcgctttcgt
    53521 ccagccgccg aactcttcat caatggtgaa taacttcaat tttggaaacg cattttcgta
    53581 ctttttcgct acctcagcgt cgcgcggacg gtagtagttt ttcgctgcaa tttcctgacc
    53641 ttctggcgag tagagatatt tcaggtaggc ttccgccacc tctttggtgc cttttttctc
    53701 gaccacttta tcgaccaccg acacggttgg ctctgcgagg atagactcac tcggcgtgac
    53761 gatttcgaat ttatctttcc ccagttcatt cgctgccagc agagcttcgt tttcccaggc
    53821 aatcagtaca tcgccaattc cgcgctcgac aaaagtgtta gtggatccac gcgcgccaga
    53881 atccagaact tcgacgtttt tatacagtgc ccgaacaaaa tcctgtgctt ttgcctgatc
    53941 gttgttgttg tgatgaagcg cgtagcccca ggctgccagg taattccagc gcgcgccacc
    54001 agagcttttc ggattaggcg tgatcaccga aacacccggt ttaatcagat cgttccagtc
    54061 atggatctgc ttcggattac ccttacgtac caggaaaaca atggtggaag tgtacggtgc
    54121 ggagttatcc ggcagacgtt tgatccactc tttatcaatc cgcccgcgtt ccgcaattgc
    54181 gtccacgtca taggccagag ccagcgtgac aacatcagct tcaataccgt tgattaccga
    54241 cgtcgcttgt ttgcctgaac caccgtgtga ctgacgaatc accacgttat cgccagtttg
    54301 ctgtttccag tgggcgctga atgccttgtt gtactgttcg tacaattcgc gcgttggatc
    54361 atatgaaacg ttaagaagct gaatatcctt tgccataacg ctggttgcca ccagcaaaaa
    54421 tgttaaccct acgccccact ttttcatcgc ccgactctct tatgttgtgt tgtgatgagc
    54481 aaagcgtgcc agaaggttga ccaaacatta aagaataaaa aaagattggc tataacttgc
    54541 gagtatatgt tgggggatta aaaaggcgga agaaatccgc ctcatattgc tgacaaagtg
    54601 cgctttgtcc atgccggatg cggcacaaac gccttatccg gcctacaaaa gtttgcaaat
    54661 tcaatagatt acagaattca tgtaggcctg ataagcgaag cgcatcaggc ctttttgctt
    54721 ctgtcatcgg tttcaggcta aaggaatctg cctttttccg aaatcattaa tacagttttt
    54781 tcgcgcagtc cagccagtcg cctttgaacg gacgcttcat gttttcgata gcgtcgatga
    54841 tgtcgtggtg aaccagctgt tcgttctgga tacctacgca acgaccaccg taacctgcca
    54901 gcagcagatc gatagcgtaa gcgcccatac gggaagccag aatacggtcg taaggcaccg
    54961 gagaaccacc gcgctggatg tggcccagca cagttgcgcg ggtttcacga ccggtttctt
    55021 tctcgatgaa atgcgccagt tcgtcaacat cacacatatg ttcggtaatc gccacgatcg
    55081 cgtgtttttt acctttcgcg atacccgctt tgatttcgtt taccaggtct tcacggctga
    55141 attcaacttc cggaaccaca acgaattcac agccaccggc aatggccgca gccaacgtca
    55201 gatcgccaca ataacggccc atcacttcca ccacggaaat acgctggtga gaagaagagg
    55261 tgtcacgcag acggtcgatc gcttctacaa cggtgctcag cgcagtgaag aaaccgatag
    55321 tgtagtcagt gcctttgatg tcgttgtcga tagtgcccgg caggccgatg cacgggaagc
    55381 ccatttcggt cagacgcatt gcacccatgt aggaaccgtc accgccgata accaccagcg
    55441 cgtcgatccc acgttttttc aggttttcga tagccacggc gcggatgttc tcgtcgcgga
    55501 attccgggaa acgcgcagaa ccgaggaacg taccgccacg gttgatcatg tcagaaacgc
    55561 tgtaacggtc tagctgtacc atacggtctt catacagacc cagatagccg tcataaatgc
    55621 ccattacttc cagaccttct gtcagcgcag aacgaacaac cccgcgaatt gcggcgttca
    55681 tgcctggcgc atcaccgccg cttgtcaaca caccgatttt cttaatcatg actacctctg
    55741 aactttggaa tgcaaaatga aatctgttgc cggaagtctt cttgcacatc gaagtgatcc
    55801 aacgaatgtg caaatagtat aacaatcact tcctgctgaa ttgattcagg tcaggccaaa
    55861 tggcggtatt ttatacacaa aatgcgggtc tggctctctt ttatactgat tatgaaagca
    55921 tagaccgttt accctccctg ggtacgacgg aacaggggtc ctgatggata atcacatccg
    55981 atcccggaaa acgccgtaaa atagcctgct ctacctgatc cgccaccata tgtgcctgaa
    56041 ccaaaggcag agagtcttcc atttccaaat gaatctgaat aaagcgggtc ggccctgact
    56101 gccgcgtgcg aagatcgtga gcgccgctaa cacccggcca ggaagtcacg atatcaataa
    56161 tttcttgccg ttcctcatca gataatgcgc gatccagtaa tgactgtacc gcctcatatc
    56221 ccatgcgtaa cgcgctatat aaaatataga tgccgatccc caatgcaaac agagcatcgg
    56281 cgcgatgcca gccgtaccag gacaacccca gcgccagcag aattgcgccg ttcatcataa
    56341 catcagactg gtaatgtagc atatcagccc gcaccgcctg gctttgcgtc cggcgcacca
    56401 cccaacgctg aaacgagaca aggataatcg tacaaattag cgccacaatt gtcacgataa
    56461 ccccgacgcc tggatctgtc atcggtgttg gagataccag atgttgaata cccgtcaaaa
    56521 acaggaatag tgccgaaccg gagataaaca tactttgcgc cagcgccgcg agggactcag
    56581 ctttaccgtg accaaacgag tgattatcgt cggcaggttg cagggaatat cgcaccacca
    56641 gtaaattcgt caacgacgca ccgatatcca ccagcgaatc caccagcgcg gcgagaatac
    56701 tcaccgaccc ggtataccac catgcaaaaa ttttaatcag caatagcagc gaagccatcg
    56761 ccgtcgcagc aatcgccgcc cgactgacca gccgtccata agattgattc ataaatactc
    56821 ccgctatcaa ctgacggtag tataacggaa gcaaatcatc tgcaatgcat taagcagcag
    56881 gcaaattgag gataaaaaaa acccccacat catgtggggg aagacaggga tggtgtctat
    56941 ggcaaggaaa acagggttta ctactgggaa cgtgagttgc tactactcaa tagcttcaac
    57001 gatgaacttt tttgccattg cgtcacgtcg cgcaactgct ccattcgttg ttgatgtttc
    57061 tcgtttaaaa ccgcttgctg ctccggcgtt aacaggcgat acatttggtt gcggactttt
    57121 gccatctcaa cctgacgagc tatttgctcg ttcgccattt tttctgcctg tgcgcgcaca
    57181 gcgttttcat caaaattttc tgcggtgaca aggcgatgca ttgtctccag ttcgctaaca
    57241 ttaacaggag gctgttcgtg ccgggcctgt tgcataagat ctcgcatctg ctgacgctga
    57301 tgttcggtta aacttatgcc gtcgaacata tggctctgcg tactgcgctg cgtaagttct
    57361 tcacccggat gccagttatc gcctgaaccg acttcagcag cgtggcttaa tgaactgact
    57421 gccagcgttg aggccatgac ggcagcggta actatgcgca tcatttgctc ccaaaatctt
    57481 tctgtcgcga ttcaacgata gagagtttac gattcaggct gcaaacatgc gtcagggggt
    57541 gtaaaacaac gtaaagtcat ggattagcga cgtctgatga cgtaatttct gcctcggagg
    57601 tatttaaaca atgaataaaa tcctgttagt tgatgatgac cgagagctga cttccctatt
    57661 aaaggagctg ctcgagatgg aaggcttcaa cgtgattgtt gcccacgatg gggaacaggc
    57721 gcttgatctt ctggacgaca gcattgattt acttttgctt gatgtaatga tgccgaagaa
    57781 aaatggtatc gacacattaa aagcacttcg ccagacacac cagacgcctg tcattatgtt
    57841 gacggcgcgc ggcagtgaac ttgatcgcgt tctcggcctt gagctgggcg cagatgacta
    57901 tctcccgaaa ccgtttaatg atcgtgagct ggtggcacgt attcgcgcga tcctgcgccg
    57961 ttcgcactgg agcgaacaac agcaaaacaa cgacaacggt tcaccgacac tggaagttga
    58021 tgccttaagt ctcaatcctg gtcgccagga agccaccttc gacgggcaaa cgctggagtt
    58081 aaccggtacg gagtttaccc tgctctattt gctggcacag catctgggtc aggtggtttc
    58141 ccgtgagcat ttaagccagg aagtgctggg caagcgcctg acgcctttcg accgcgctat
    58201 cgatatgcat atttccaacc tgcgtcgtaa actgccggat cgtaaagatg gtcacccgtg
    58261 gtttaaaacc ctgcgtggtc gcggctatct gatggtttct gcttcatgat aggcagctta
    58321 accgcgcgca tcttcgccat cttctggctg acgctggcgc tggtgttgat gttggttttg
    58381 atgttaccca agctcgattc acgccagatg accgagcttc tggatagcga acagcgtcag
    58441 ggtctgatga ttgagcagca tgtcgaagcg gagctggcga acgatccgcc caacgattta
    58501 atgtggtggc ggcgtctgtt ccgggcgatt gataagtggg caccgccagg acagcgtttg
    58561 ttactggtga ccaccgaagg ccgcgtgatc ggcgctgaac gcagcgaaat gcagatcatt
    58621 cgtaacttta ttggtcaggc cgataacgcc gatcatccgc agaagaaaaa gtatggccgc
    58681 gtggaactgg tcggtccgtt ctccgtgcgt gatggcgaag ataattacca actttatctg
    58741 attcgtccgg ccagcagttc tcaatccgat ttcataaact tactgtttga ccgcccgcta
    58801 ttactgctga ttgtcaccat gctggtcagt acgccgctgc tgttgtggtt ggcctggagt
    58861 ctggcaaaac cggcgcgtaa gctgaaaaac gctgccgatg aagttgccca gggaaactta
    58921 cgccagcacc cggaactgga agcggggcca caggaattcc ttgccgccgg tgccagtttt
    58981 aaccagatgg tcaccgcgct ggagcgcatg atgacctctc agcagcgtct actttctgat
    59041 atctctcacg aactgcgcac cccgctgacg cgtctgcaac tgggtacggc gttactgcgc
    59101 cgtcgtagcg gtgaaagcaa ggaactggag cgtattgaaa ccgaagcgca acgtctggac
    59161 agcatgatca acgatctgtt ggtgatgtca cgtaaccagc agaaaaatgc gctggttagc
    59221 gagaccatca aagccaacca gttgtggagt gaagtgctgg ataacgcggc gttcgaagcc
    59281 gagcaaatgg gcaagtcgtt gacggttaac ttcccacctg ggccgtggcc gctgtacggc
    59341 aacccgaacg ccctggaaag tgcgctggaa aacattgttc gtaatgctct gcgttattcc
    59401 catacgaaga ttgaagtggg ctttgcggta gataaagacg gtatcaccat tacggtggac
    59461 gacgatggtc ctggcgttag cccggaagat cgcgaacaga ttttccgtcc gttctatcgg
    59521 accgatgaag cacgcgatcg tgaatctggc ggtacaggtt tggggctggc gattgttgaa
    59581 accgccattc agcagcatcg tggctgggtg aaggcagaag acagcccgct gggcggttta
    59641 cggctggtga tttggttgcc gctgtataag cggagttaaa ctccgcattt gtaggctgga
    59701 taaggcgttt acgccgcatc cggcatttga gcaggatgcc tgatgcgacg ctgatagcgt
    59761 cttatcaggc ctacactccg gggttatttt ccccacaaac ggcgggaaaa atcttcgatc
    59821 ttgccgctca gtcggcgctt ttgcatcgtt cgcgtccagc ttttcgataa cccggcggcg
    59881 gagagtaaac ggtgatactg atcgtcatca aacggcatat gccatgcgat ggcggcagcc
    59941 tcctgcacgg tcacatcgct gacacgggaa accaactcca gcggcgcatc agcagatacc
    60001 aaccctggtg caattacgct atacaaccac cccactttac ccgtgttttg catcaactgc
    60061 gctatgtcgc tgatatcaaa atggtaattg agtttatagc aaggtgagcg tggctggctg
    60121 acctgaatta atgcctctcc ccagcggaaa atatcaccaa tataaacatt actttccgtc
    60181 agaccgtcgg ttgagagatt ttcgccaaac gcaggcgcaa caaacagctc cgcctgttcc
    60241 ggaaattccc gcgcccagta gagataatgc tcacgaggat aatgacacag cgctctgtct
    60301 ggcccgccgt ggatcttttt ctccgcctgt tcgtcacctt ccagccccag gtccgtcagc
    60361 atcaactcac catcaacctg gattttggca attgcgctgg gtttgccttc gggataagcc
    60421 tgaatcttgc ctgtgtatac atcaaccgga tatcgcatct gttccctctc cacgagaaat
    60481 ttccccatta gaccatattt attttatgtc aggcgtgctg ttttcgcggt aagtatccag
    60541 gcagaaacgc aattgaaaac cggacgaacg ttagttcatc cggctgtggg gattaaggaa
    60601 gctggtttat ttcaccgtac ctaaaatttc gattttcgcc gctctggctt tgactttacg
    60661 tgaccagata gaggtaagaa ttggcaccag aatcgaagtc acaatgaccg ccgtcgcgac
    60721 cagcgaagtt gctgccggag ccataggttt aaacgcaggc accatttcag caatcagcac
    60781 aggtgtcgct accgcggccc ctgcggaact ggaagcggca attccggcag tgccatcgcc
    60841 accgccaatc aatttatcgg cgataatcaa cggaataccg gtcacgataa ttactgccac
    60901 acccaacaga atccccagca aacctgtctg ggcaattaca gtcaaatcaa tggtattgcc
    60961 cagtgcgaag gcaaagaatg gaatcagcgt ctgcaccgct ttgctgaaaa attctcgtaa
    61021 ctcagggtca aggttcccca gggcaaagcc caccaggaac ggcaatactg cgccgacgaa
    61081 aacatgcggt tcaaacgagg caatcccggc agtgcccaga ataatcatcg tcatgagcgg
    61141 cccggactcc aacgacatca acacaaatgc cccagcttct tcttttgtgc cgtactgctg
    61201 catgatggaa gcgtaaagtc cgccgttggt catatccatc gccgccacca gcgccaacgt
    61261 tgaaagtccg gcaaaaaatc caacttcaac accatgttcc ggaataatgc gcgaggcaat
    61321 tgccgcaacc acccacgcga cggcaatttt agttaccacc agcgtaccgg atttacgcag
    61381 taccgttccc gtcgcgctta attttattga cgcccccatg caaaaaaacc acaccgccag
    61441 aatgggcacc gtaccggtaa tcatcccgtt ggtgaatgat ccaaaatatt tccccgcctc
    61501 cggcgagaag gtgtggcaca gtgcgccaag gaatagcggg acgagcatca tcccccccgg
    61561 gattttctca atcgagcgtt ttatctgcat ttccatcacc tgtcggcata tttttaaggg
    61621 ctgatgcgtc attcctcaac ggagcatcag tgttgatgga aacgaagata aacattcatt
    61681 ttatcaataa aagtgatcga aaccacatca attgaaacgc tgttttattt ttatcggatc
    61741 attgttttta ttttgaaatt gatcacaaaa aaaccaccgc cgttggcgat ggttcatttt
    61801 ttaagctgca atgcggcgta aacgcctcat tgcagcaggc gtcaaatgat tattttttcg
    61861 ccgcgaaacg tgccgctgct tcgtcccagt tcaccacgtt ccagaactct ttgatgtagt
    61921 ccggacgacg gttctggaat ttcagatagt aagcatgttc ccacacatcc aggcccagaa
    61981 tcgggaagcc ggaagcgcca gaaatagctt cacccatcag cggggagtcc tggttagcag
    62041 tagaaaccac cgccagttta tcgccttttt gcaccagcca tgcccagccg gaaccaaagc
    62101 gggaagctgc cgctttctca aattctgctt tgaagttatc aacggagccg aagtcacgtt
    62161 cgatagccgc tttcaggtca ccctgcaggg tggtgccttt tttcagacct ttccagaaca
    62221 ggctgtggtt agcgtgaccg ccagcgttat tgcgcagtac ggttttcttg tctgctggca
    62281 gctggtccag tttggtgatc agctcttcaa ccggcaggtt agcgaattct ggcaggcttt
    62341 ccagcgccgc gttggcgttg ttgacgtaag tctgatggtg tttggtgtgg tggatttcca
    62401 tggtctgctt atcgaagtgc ggttccaggg catcgtaagc atacggcagg gatggcaggg
    62461 tataactcat attcatctcc agtattgtcg ggcggccgat tgttaatgcc gcgtaagcag
    62521 ttggttcatt atagttaatt aaatgatatt gaaaatgatt atcaatgccg tacttttcgt
    62581 aagggtatgg ttttgcagga aaatgcccga gatgtgaagc aaatcaccca cttaatgccg
    62641 tgattgccag taaatcgaca acggcggcaa caggcgaaag gttaatcgac agcacgattt
    62701 ttacactcat ctcgtcggag atgtgacgcg acgaaaaatg atgaggataa gaaaatgagt
    62761 aacgcgatta cgatggggat attttggcat ttgatcggcg cggccagtgc agcctgtttt
    62821 tacgcgccgt tcaaaaaagt aaaaaagtgg tcatgggaaa ccatgtggtc agtcggtggg
    62881 attgtttcgt ggattatcct gccgtgggcc atcagcgccc tgttactacc gaatttctgg
    62941 gcgtattaca gctcatttag tctctctacg ctgctgcctg tttttctgtt cggcgctatg
    63001 tgggggatcg gtaatatcaa ctacggcctg accatgcgtt atctcggcat gtcgatggga
    63061 attggcatcg ccattggcat tacgttgatt gtcggtacgc tgatgacgcc aattatcaac
    63121 ggcaatttcg atgtgctgat taataccgaa ggcggacgca tgacgttgct cggcgttctg
    63181 gtggcgctga ttggcgtagg gattgtaact cgcgccgggc agttgaaaga gcgcaagatg
    63241 ggcattaaag ccgaagagtt caatctgaaa aaagggctgg tgctggcggt gatgtgcggc
    63301 attttctctg ccgggatgtc ctttgcgatg aacgccgcaa aaccgatgca tgaagccgct
    63361 gccgcgctgg gcgtcgatcc cctgtatgtc gctttgccaa gctatgttgt catcatgggc
    63421 ggcggcgcaa tcatcaacct cggcttctgc ttcattcgtc tggccaaagt gaaagatttg
    63481 tcgctaaaag ccgacttctc gctggcaaaa ccgctgatca ttcacaatgt gttactctca
    63541 gcactgggcg ggttgatgtg gtatctgcaa ttctttttct atgcctgggg ccacgcccgc
    63601 attccggcgc agtatgacta catcagctgg atgctgcata tgagcttcta tgtattgtgc
    63661 ggcggtatcg tcgggctggt gctgaaagag tggaacaatg caggacgccg tccggtaacg
    63721 gtgttgagcc tcggttgtgt ggtgattatt gtcgccgcta acatcgtcgg catcggcatg
    63781 gcgaattaat ctttctgcga attgagatga cgccactggc tgggcgtcat cccggtttcc
    63841 cgggtaaaca ccaccgaaaa atagttacta tcttcaaagc cacattcggt cgaaatatca
    63901 ctgattaaca ggcggctatg ctggagaaga tattgcgcat gacacactct gacctgccgc
    63961 agatattgat tgatggtcat tccagtctgc tggcgaaatt gctgacgcaa aacgcgctca
    64021 ctgcatgatg cctcatcaca aaatttatcc agcgcaaagg gacttttcag gctagccgcc
    64081 agccgggtaa tcagcttatc cagcaatgtt tcgctggatg ttggcggcaa cgaatcactg
    64141 gtgtaacgat ggcgattcag caacatcacc aactgcccga acagcaactc agccatttcg
    64201 ttagcaaacg acacatgctg actactttca tgctcaagct gaccgataac ctgccgcgcc
    64261 tgcgccatcc ccacgctacc taagcgccag tgtggttgcc ctgcgctggc gctaaatccc
    64321 ggaatcgcgc cctgccagtc aagattcagc ttcagccgct ccgggcaata aataatattc
    64381 tgcaaaacca gatcgttaac ggaagcgtag gagtgtttat cgtcagcatg aatataaaag
    64441 agatcgccac gggtaatgcg ataagggcga tcgttgagta catgcagacc attaccgcgc
    64501 cagacaatca ccagctcaca aaaatcatgt gtatgttcgg caaagacatc ttgcggataa
    64561 cggtcagcca cagcgactgc ctgctggtcg ctggcaaaaa aatcatcttt gagaagtttt
    64621 aactgatgcg ccaccgtggc tacctcggcc agagaacgaa gttgattatt cgcaatatgg
    64681 cgtacaaata cgttgagaag attcgcgtta ttgcagaaaa ccatcccgtc cctgacgaat
    64741 atcacgcggt gaccagttaa actctcggcg aaaaagcgtc gaaaagtggt tactgtcgct
    64801 gaatccgcag cgataggcga tgtcagtaac gctggcctcg ctgtggcgta gcagatgtcg
    64861 ggctttcatc agtcgcaggc ggttcaggta acgctgaggc gtcagccccg tttgctgctt
    64921 aagctggcga tgcagcgtac gcaatgaaag agaaaattga tccgccacag cgtcccaatt
    64981 cacctcatcg gcaaaatggt cctccagcca ggccagaagc aagttgagac gtgatgcgct
    65041 gttttccagg ttctcctgca aactgctttt acgcagcaag agcagtaatt gcataaacag
    65101 aatttcacga ctggcggtcg agggtaaatc attttcccct tcctgctgtt ccatctgtgc
    65161 aaccagctgt cgcacctgct gcaatacgct gtggttaacg cgccagtgag acggatactg
    65221 cccatcctgc tcttgtggca gcaactgatt cagcccggcg agaaactgaa atcgatccgg
    65281 cgagcgatac agcacattgg tcagacacag attatcggta tgttcataca gatgccgatc
    65341 atgatcgcgt acgaaacaga ccgtcccacc ggtgatggta tagggctgcc cattaaatac
    65401 atgaataccc gtgccatgtt cgacaatcac aatttcatga aaatcatgat gatgttcagg
    65461 aaaatccgcc tgcggaagcc ggggttctat cgccacggac gcgttaccag acggaaaaaa
    65521 atccacacta tgtaatacgg tcatactggc ctcctgatgt cgtcaacacg gcgaaatagt
    65581 aatcacgagg tcaggttctt accttaaatt ttcgacggaa aaccacgtaa aaaacgtcga
    65641 tttttcaaga tacagcgtga attttcagga aatgcggtga gcatcacatc accacaatga
    65701 agcaaattgt gaacatcatc acgttcatct ttccctggtt gccaatggcc cattttcctg
    65761 tcagtaacga gaaggtcacg aattcaggcg ctttttagac tggtcgtaat gaaattcagc
    65821 aggatcacat tatgaccttt cgcaattgtg tcgccgtcga tctcggcgca tccagtgggc
    65881 gcgtgatgct ggcgcgttac gagcgtgaat gccgcagcct gacgctgcgc gaaatccatc
    65941 gttttaacaa tggcctgcat agccagaacg gttatgtcac ctggaatgtg gatagcctgg
    66001 aaagtgccat tcgccttgga ttaaacaagg tgtgcgagga agggattcgt atcgatagca
    66061 ttgggattga tacctggggc gtggactttg tgctgctcga ccagcacggt cagcgcgtgg
    66121 gtctgcccgt tgcttaccgc gatagccgca ccaatggcct aatggcgcag gcacaacagc
    66181 aactcggcaa acgcgatatt tatcaacgta gcggcatcca gtttctgccc ttcaatacga
    66241 tctatcagct acgtgcactg acagagcagc aaccagaact tattccacac attgctcacg
    66301 ctttgctgat accggattac tttagttatc gcctgaccgg caagatgaac tgggaatata
    66361 ccaacgccac gaccacgcaa ctggtcaata tcaatagcga cgactgggac gagtcgctac
    66421 tggcgtggag cggtgccaac aaagcctggt ttggtcgccc gacgcatccg ggtaatgtca
    66481 taggtcactg gatttgcccg cagggtaatg agattccggt ggtcgccgtt gccagccatg
    66541 ataccgccag cgcggttatc gcctcgccgt taaacggttc acgcgccgct tatctctctt
    66601 ctggcacctg gtcattgatg ggcttcgaaa gccagacgcc atttaccaat gacacggcgc
    66661 tggcagccaa tatcaccaac gaaggcgggg cggaaggtcg ctatcgggtg ctgaaaaata
    66721 ttatgggctt atggctgctt cagcgagtgc ttcaggagcg gcaaatcaac gatcttccgg
    66781 cgcttatcgc cgcaacacag gcacttccgg cctgccgctt catcatcaat cccaatgacg
    66841 atcgctttat taatcctgac gagatgtgca gcgaaattca ggctgcgtgt cgggaaacgg
    66901 cgcaaccgat cccggaaagt gatgctgaac tggcgcgctg tattttcgac agtctggcgc
    66961 tgctgtatgc cgatgtgttg catgagctgg cgcagctgcg tggtgaagat ttctcgcaac
    67021 tgcatattgt cggcggcggc tgccagaaca cgctgctcaa ccagctgtgt gccgatgcct
    67081 gcggtattcg ggtgatcgcc gggcctgttg aagcctcgac gctcggcaat atcggcatcc
    67141 agttaatgac gctggatgaa ctcaacaatg tggatgattt ccgtcaggtc gtcagcacta
    67201 ccgcgaatct gaccaccttt acccctaatc ctgacagtga aattgcccac tatgtggcgc
    67261 agattcactc tacacgacag acaaaggagc tttgcgcatg accactcaac tggaacaggc
    67321 ctgggagctg gcgaaacagc gtttcgcggc agtagggatt gatgtcgagg aggcactgcg
    67381 ccaacttgat cgtttacccg tttcaatgca ctgctggcag ggcgatgatg tttccggttt
    67441 tgaaaacccg gaaggttctc tgaccggtgg tattcaggct actggtaatt atccgggcaa
    67501 agcgcgtaat gccagcgagc tgcgtgccga tctggaacag gctatgcggc tgattccggg
    67561 gccaaaacgg cttaatttgc atgccattta tctggagtca gacacgccag tctcacgtga
    67621 ccaaatcaaa ccagagcact tcaaaaactg ggttgaatgg gcgaaagcca atcagctcgg
    67681 tctggatttt aacccctcct gtttttcgca tccgctaagc gccgatggct ttacgctttc
    67741 ccatgccgac gaccgcattc gccagttctg gattgaccac tgtaaagcca gccgccgtgt
    67801 ttcagcctat tttggtgagc aactcggcac accgtcggtg atgaacatct ggatcccgga
    67861 tggcatgaaa gatatcaccg ttgaccgtct cgccccgcgt cagcgtttgc tggcagcact
    67921 ggatgaagtg atcagcgaga agctaaaccc tgcgcaccat atcgacgccg ttgagagcaa
    67981 attgtttggc attggcgcgg agagctacac ggttggctcc aatgagtttt acctggggta
    68041 tgccaccagc cgccagacgg cgctgtgcct ggacgccggg catttccacc cgactgaagt
    68101 gatttccgac aagatttccg ccgccatgct gtatgtgccg cagttgctgc tgcacgtcag
    68161 ccgtccggtt cgctgggaca gcgatcacgt agtgctgctg gatgatgaaa cccaggcgat
    68221 tgccagtgag attgttcgtc acgatctgtt tgaccgggtg catatcggcc tcgacttctt
    68281 cgatgcctct atcaaccgca ttgccgcgtg ggtcattggt acacgcaata tgaaaaaagc
    68341 cctgctacgt gcgttactgg aacctaccgc tgaactacgc aagctggaat cggcgggcga
    68401 ttacactgcg cgtctggcgc tactggaaga gcagaaatcg ttgccgtggc aggcggtatg
    68461 ggaaatgtat tgtcaacgtc acgatacgcc agcgggtagc gaatggctgg agaacgtgcg
    68521 gacttatgag aaaaaaattt tgagtcgccg cgggtaaaca ctgccggatg aggcacaagt
    68581 gccttatccg gcctacaggt cgtgcatccg acaacaccga attaacagga acacagaaca
    68641 tgcaaaacat aacccagtcc tggtttgtcc agggaatgat caaagccacc accgatgcct
    68701 ggctgaaagg ctgggacgag cgcaacggcg gcaacctgac gctgcgcctg gatgacgccg
    68761 atatcgcacc atatcacgac aattttcacc aacaaccgcg ctatatcccg ctcagccagc
    68821 ccatgccttt actggcaaat acaccgttta ttgtcaccgg ctccggcaaa ttcttccgta
    68881 acgtccagct tgatcctgcg gctaacttag gcatcgtaaa agtcgacagc gacggcgcgg
    68941 gctaccacat tctttggggg ttaaccaacg aagccgttcc cacttctgaa cttccggctc
    69001 actttctctc ccactgcgag cgcattaaag ccaccaacgg taaagatcgg gtgatcatgc
    69061 actgccacgc caccaacctg atcgccctca cctatgtact tgaaaacgac accgcggtct
    69121 tcactcgcca actgtgggaa ggcagcaccg agtgtctggt ggtattcccg gatggcgttg
    69181 gcattctgcc gtggatggtg cccggcacgg acgaaatcgg ccaggcaact gcacaagaga
    69241 tgcaaaaaca ttcgctggtg ttgtggccct tccacggtgt gttcggcagc gggccgacgc
    69301 tggatgaaac cttcggttta atcgacaccg cagaaaaatc agcacaagta ttagtgaagg
    69361 tttattcgat gggcggcatg aaacagacca tcagccgtga agagttgata gcgctcggcc
    69421 agcgtttcgg cgttacacca ctcgccagtg cgctggcgct gtaaggagca aacatgatcc
    69481 gcaaagcctt tgtcatgcag gtaaaccccg acgcccacga agagtatcag cgtcggcata
    69541 atcccatctg gccggaactg gaagccgtgc tgaaatctca cggtgcgcat aactacgcca
    69601 tctatctcga caaagcgcgt aatctgctat ttgccacgat agagattgaa tctgaagagc
    69661 ggtggaatgc ggttgccagt acagatgttt gccaacgttg gtggaaatat atgaccgacg
    69721 ttatgcccgc taatgcggat aacagcccag tcagtagcga actgcaagaa gtgttttacc
    69781 tgccttaatg agttgcggtg ccggaaaata tccagcaccg ccctttcatc agaagaagta
    69841 tttaaagcga acgcgtgcgc cgtaacggtc atcactatcg tcgccgtgta tcttatcgcc
    69901 gtcgctatcc agaatggagt aatacgtgcc gaggtaaata ttaaagttct ccatatccat
    69961 cacattagcg aactgataag acgcatgaat ggtgtgaatg ttgtatgttc cttcatcatc
    70021 aatccagcaa tcgttatcac aaaccacgcc actaaattca tcaatcttat tatgtgcgta
    70081 gatataaccc agctcgaaac gtttccacag cgcgttaatc ccagcggtga aatctttctc
    70141 attattagca tctaaatagg cggtattaag attaaccacg atgccatttt ccggatcggt
    70201 tttcaggcca ttccaggtca tgctcatgcc ataaccggta cgatcggact gatcgacaaa
    70261 attacccttg ctatcggtat aaccataagc attatttacc acgttcgctt ccatcgctgc
    70321 ggaaacggtg aattcttccg tcggcgacca ggcaattacc ggacgcagat aagcaacatt
    70381 tttctgctgt tccatatcgc gtccatgata attaccgtcg ttatataaag atgttccgtc
    70441 ttccagtaac gtgtttaatt caaaatacca gttatcgagt tgtttactga cgaggaaatt
    70501 accgccagcg ttagaacgcc cgcggccctc tttcatcata tagatataac cgctgccatc
    70561 gtcataaaga tcgttcgcag tattaccgga atgttcaaca aaggtatcct gattcagcgg
    70621 gaacatatcg taggcttcaa aacggcctac tttgaccttc cagtcgtttt ctttgccaaa
    70681 gaagaaaacc gcatcatcga tatttactga accgtgcata tcccccagcg gttgtgcgga
    70741 gaacccggcg aaatagccat tatccagttt tcgcatacca tcaaaaccta acagaatacg
    70801 accattgaga ttccactgtt cattagtggg atcgctattc acatcagcgt tggtcattgc
    70861 cagcaggcca tgattacttt ccgcatccat attaaattct acgtcaccgt agatttttaa
    70921 atcgccatac cctgaaagca ggagattagg cggggtggcg tttttcttct ctgactccgt
    70981 cgttgattga ccgtttaccg gcgtgttgcc ctgtgcggtt ttaatttcgc ggatctcggc
    71041 ggcttgttgt gtctgaagct gtttaacctg cccctctgct tttgctgccc gcatttctgc
    71101 cacttccagg cgttcttcca gcaatgccat gcgttgttcc agcgttaaat gctgtgtttt
    71161 tgccataccg gtactacatg ccaataataa accgatggct aaagataatg tcgatttttt
    71221 cattatttac gttccctgaa ataaaggaga ccctacgctt tccaaaagga atacgtatat
    71281 agagaaatat aaggtgtgca ctttcgaatt tttaatccaa cttcagcagg ccgatggata
    71341 gtatcccgcc atataatatg agtaaatccg ctgatttcct ggaacgtgag gaatataaca
    71401 atagtgccgt ttttaactaa atatcgtaaa aaatattaga tatgattcac gctga
//
LOCUS       DAHHTG010000007        60035 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.007, whole genome shotgun sequence.
ACCESSION   DAHHTG010000007 DAHHTG010000000
VERSION     DAHHTG010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 60035)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 60035)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..60035
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.007"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            113..679
                     /locus_tag="NBI74_000487"
     CDS             113..679
                     /locus_tag="NBI74_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCD1930863.1"
                     /translation="MNKKLLSLLVSGSLIAVVSTSVFATDTGVVNFNGKIVADTCEIN
                     VNESASTTGTVTFADTYPADYSGDGSIGTSKDFKIELIKCDPLVDKLNLTFSGTTTDA
                     GFLRLENSEAGAGSATNVGITVTNKNGGANDVKFDGSVPDASTDVDNAGSITPTIFNY
                     TANVIQVGNNAPTAGKYASSATFEVFYR"
     gene            725..1441
                     /locus_tag="NBI74_000488"
     CDS             725..1441
                     /locus_tag="NBI74_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000110509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HCD1930864.1"
                     /translation="MSVFSRVCLGFTFLLTALQVQASIVLGGTRIIYPSNQNEVQITL
                     KNKDAYARYLVQSWVSNIDGSKAPFLITPPVYKLEENRQTLLHIVFTGDKNKLPQDRE
                     SLFLANIKSVSAMPEELKDKNTLQFAMKARLKLFWRPASLDNSDALTAWEKLKFHKEA
                     GKLIVKNPTPFYISFSDLTVSGKNIVPTESKSEPGALLMMVGPFSEQRFSLPVGAGSI
                     VEWSVITDYGSSSGKRQQKL"
     gene            1508..3985
                     /locus_tag="NBI74_000489"
     CDS             1508..3985
                     /locus_tag="NBI74_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001606829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HCD1930865.1"
                     /translation="MLLPLHRTFVLTGITFALSAVYSLSYARDEFNLRILELDSPLEN
                     TQVLEDFVNNNNLTPGVYLTSVMWGQEYLDKRNITFILSSDKKRLIPRFTKADLREFG
                     LKVDDIPALQVMDDDTEFGDIAQIIDGARYDFQLDSQTLCLRIPQIYQNARAAGSISP
                     KYWSDGESAVWLSYYASGSRQNSDGDNLNSNWLNLNSGINLGVWRLRNNTVYSDSSWE
                     SISTSLQRDIKALRSQMEVGQTFTNGDLFDSVQMTGIKLETDTSMLPDSEQGFAPVVR
                     GIANSDAQVVIKQNGYVIYQTWVSAGPFEIKDLSQVTAGADLEVTIKETNGQEHSFIQ
                     ASSTVPILQREGALKYSLATGKYRDNDNHAETPVFGVATAIYGLPYGITIYGGILGAS
                     IYHSGVTGIGADLGRLGSVSVDITAAETKFDDGRDDATGLSWRAQYAKDFPDTDTTVT
                     LASYRYSTSQFYTFQEALDQRDTPDDKGIYSYRQTNNRRNRLQINLSQNIGRWGSVYL
                     NGYQQDYWGMHGAERSIGMGYSTTWSNINWSVNYTLTKTPGMAGEQQFSLTLNIPLSR
                     WLPDSWAMYNVNRSDKSNTSHQLGIGGTALQDNNLSYNLQQSYTDNNVGYDASMNGRY
                     RSSVGEFGLGYSYDKNSRQWNYSAQGAVVAHAHGVTLGQSVQDSFAIVHINEGANVKV
                     QNAQGVYTDYWGNAIVPNMTNYRHNAITVNTQGHDSLDISDATQDVIPSKGAVVGVDF
                     DARSGIRALLTLVHNKERVPFGALLTLGNSTAIVGEDGEVYITGVQESMTFTVQWGKE
                     INQQCTGVVTVPEKYTTGIYKATMDCR"
     gene            4007..5017
                     /locus_tag="NBI74_000490"
     CDS             4007..5017
                     /locus_tag="NBI74_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCD1930866.1"
                     /translation="MKPGFVSYIRTLLTIIFLSWGVIPEVFAGCNISGNGGNPATVQF
                     SVPALVVNSDAVPGTIIATVNANSPSISVSCTANGEIWQGYTVLTNGDMRTDNPLNNV
                     FQTNVPGIGFRAAWANNTSAQLTAGYLMTPWHKGSSTVIKGYGYPITIHAAIQFIVTG
                     PVGNGTIDTSKLVADWKYDNRVIGELRFSSVPVNIQPGTCDLIEKNITVPLNDIGTDD
                     FDVNNVSPVVSDDRFKIQIEKCSQDVKVDYRFTSSGSTGVTGNNILNIESGSGMAEGV
                     GLQILDSNNNVMVFDTDYTPVQKTTLNQNVTIPLKARYIKMGSVKSGRVNSVATFEVY
                     YR"
     gene            5237..7486
                     /gene="fhuA"
                     /locus_tag="NBI74_000491"
     CDS             5237..7486
                     /gene="fhuA"
                     /locus_tag="NBI74_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrichrome porin FhuA"
                     /protein_id="HCD1930867.1"
                     /translation="MARSKTAQPKHSLRKIAVVVATAVSGMSVYAQAAVEPKEDTITV
                     TAAPAPQESAWGPAATIAARQSATGTKTDTPIQKVPQSISVVTAEEMALHQPKSVKEA
                     LSYTPGVAVGTRGASNTYDYLIIRGFAADGQSQNNYLNGLKMQGNFYNDAVIDPYMLE
                     RAEIMRGPVSVLYGKSSPGGLLNMVSKRPTTEPLKEVQFKAGTDSLFQTGFDFSDALD
                     DDGVYSYRLTGLARSANAQQKGAEEQRYAIAPAFTWRPNDKTNFTFLSYFQNEPETGY
                     YGWLPKEGTVEPLPNGKRLPTDFNEGAKNNTYSRNEKMIGYSFDHEFNDTFTVRQNLR
                     FAQNKVSQKSVYGYGMCSDPLYSSNPSSSPCANVPQSQWGHTLTRQYVIDNEKLENFS
                     VDTQLQSKFATGSVDHTLLTGVDFMRMRNDIDSWFGYAGSVAPSDIYNLDRSDFDFGA
                     HPNPSGPYRVLLKQKQTGLYVQDQAQWDKVLVTLGGRYDWADQSSFNRDYGNKSERDD
                     KEFTWRGGVNYLFDNGVTPYFSYSESFEPASQTDANGDLFAPSKGKQYEVGVKYVPED
                     RPIVVTGALYQLTKTNNLMADPNGSLFSVEGGEIRARGVELEAKAALSASVNLVGSYT
                     YTDAEYTTDTNYKGNTPAQVPKHMASLWADYTFFDGPLSGLTLGTGGRYTGSSYGDPA
                     NSFKVGSYTVVDALVRYDLARVGMAGSNVALHVNNLFDREYVASCFNTYGCFWGAERQ
                     VVATATFRF"
     gene            7538..8335
                     /gene="fhuC"
                     /locus_tag="NBI74_000492"
     CDS             7538..8335
                     /gene="fhuC"
                     /locus_tag="NBI74_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe3+-hydroxamate ABC transporter ATP-binding
                     protein FhuC"
                     /protein_id="HCD1930868.1"
                     /translation="MQEYTNHSDTTFALRNISFRVPGRTLLHPLSLTFPAGKVTGLIG
                     HNGSGKSTLLKMLGRHQPPSEGEILIDAQPLESWSSKAFARKVAYLPQQLPPAEGMTV
                     RELVAIGRYPWHGALGRFGVADREKVEEAISLVGLKPLAHRLVDSLSGGERQRAWIAM
                     LVAQDSRCLLLDEPTSALDIAHQVDVLALVHRLSQERGLTVIAVLHDINMAARYCDYL
                     VALRGGEMIAQGTPAEIMRGENLEMIYGIPMGILPHPAGAAPVSFVY"
     gene            8335..9225
                     /gene="fhuD"
                     /locus_tag="NBI74_000493"
     CDS             8335..9225
                     /gene="fhuD"
                     /locus_tag="NBI74_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter
                     substrate-binding protein FhuD"
                     /protein_id="HCD1930869.1"
                     /translation="MSGLPLISRRRLLTAMALSPLLWQMNTAHAAVIDPNRIVALEWL
                     PVELLLALGIVPYGVADTINYRLWVSEPPLPDSVIDVGLRTEPNLELLTEMKPSFMVW
                     SAGYGPSPEMLARIAPGRGFNFSDGKQPLAMARKSLTEMADLLNLQSAAETHLAHYED
                     FIRSMKPRFVKRGARPLLLTTLIDPRHMLVFGPNSLFQEILDEYGIPNAWQGETNFWG
                     STAVSIDRLAAYKDVDVLCFDHDNSKDMDALMATPLWQAMPFVRAGRFQRVPAVWFYG
                     ATLSAMHFVRILDNAIGGKA"
     gene            9222..11204
                     /gene="fhuB"
                     /locus_tag="NBI74_000494"
     CDS             9222..11204
                     /gene="fhuB"
                     /locus_tag="NBI74_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter permease
                     FhuB"
                     /protein_id="HCD1930870.1"
                     /translation="MSKRIALFPVLLLALLVVAAAALTWMNFSQALPRSQWAQAAWSP
                     DIDVIEQMIFHYSLLPRLAISLLVGAGLGMVGVLFQQVLRNPLAEPTTLGVATGAQLG
                     ITVTTLWAIPGAMASQFAALAGACVVGLIVFGVAWGKRLSPVTLILAGLVVSLYCGAI
                     NQLLVIFHHDQLQSMFLWSTGTLTQTDWGGVERLWPQLLGGVMLTLLLLRPLTLMGLD
                     DGVARNLGLALSLARLAALSLAIVISALLVNAVGIIGFIGLFAPLLAKMLGARRLLPR
                     LMLASLIGALILWLSDQIILWLTRVWMEVSTGSVTALIGAPLLLWLLPRLRSISAPDM
                     KVNDRVATERQHVLAFALAGGVLLLMAVVVALSFGRDAHGWTWASGALLEDLMPWRWP
                     RIMAALFAGVMLAVAGCIIQRLTGNPMASPEVLGISSGAAFGVVLMLFLVPGNAFGWL
                     LPTGSLGAAVTLLIIMIAAGRGGFSPHRMLLAGMALSTAFTMLLMMLQASGDPRMAQV
                     LTWISGSTYNATDAQVWRTGIVMVILLAITPLCRRWLTILPLGGDTARAVGMALTPTR
                     IALLLLAACLTATATMTIGPLSFVGLMAPHIARMMGFRRTMPHIVISALVGGLLLVFA
                     DWCGRMVLFPFQIPAGLLSTFIGAPYFIYLLRKQSR"
     gene            complement(11239..12519)
                     /gene="hemL"
                     /locus_tag="NBI74_000495"
     CDS             complement(11239..12519)
                     /gene="hemL"
                     /locus_tag="NBI74_000495"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HCD1930871.1"
                     /translation="MSKSENLYSAARELIPGGVNSPVRAFTGVGGTPLFIEKADGAYL
                     YDVDGKAYIDYVGSWGPMVLGHNHPAIRNAVIEAAERGLSFGAPTEMEVKMAQLVTEL
                     VPTMDMVRMVNSGTEATMSAIRLARGFTGRDKIIKFEGCYHGHADCLLVKAGSGALTL
                     GQPNSPGVPADFAKHTLTCTYNDLASVRAAFEQYPQEIACIIVEPVAGNMNCVPPLPE
                     FLPGLRALCDEFGALLIIDEVMTGFRVALAGAQDYYGVEPDLTCLGKIIGGGMPVGAF
                     GGRRDVMDALAPTGPVYQAGTLSGNPIAMAAGFACLNEVAQPGVHETLDELTTRLAEG
                     LREAAEEAGIPLVVNHVGGMFGIFFTDAESVTCYQDVMSCDVERFKRFFHMMLDEGVY
                     LAPSAFEAGFMSVAHSMEDINNTIDAARRVFAKL"
     gene            12744..14165
                     /gene="clcA"
                     /locus_tag="NBI74_000496"
     CDS             12744..14165
                     /gene="clcA"
                     /locus_tag="NBI74_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="H(+)/Cl(-) exchange transporter ClcA"
                     /protein_id="HCD1930872.1"
                     /translation="MKTDTPSLETPQAARLRRRQLIRQLLERDKTPLAILFMAAVVGT
                     LVGLAAVAFDKGVAWLQNQRMGALVHTADNYPLLLTVAFLCSAVLAMFGYFLVRKYAP
                     EAGGSGIPEIEGALEDQRPVRWWRVLPVKFFGGLGTLGGGMVLGREGPTVQIGGNIGR
                     MVLDVFRLKGDEARHTLLATGAAAGLAAAFNAPLAGILFIIEEMRPQFRYTLISIKAV
                     FIGVIMSTIMYRIFNHEVALIDVGKLSDAPLNTLWLYLILGIIFGIFGPIFNKWVLGM
                     QDLLHRVHGGNITKWVLMGGAIGGLCGLLGFVAPATSGGGFNLIPIATAGNFSMGMLV
                     FIFVARVITTLLCFSSGAPGGIFAPMLALGTVLGTAFGMVAVELFPQYHLEAGTFAIA
                     GMGALLAASIRAPLTGIILVLEMTDNYQLILPMIITGLGATLLAQFTGGKPLYSAILA
                     RTLAKQEAEQLARSKAASASENT"
     gene            14189..14254
                     /locus_tag="NBI74_000497"
     CDS             14189..14254
                     /locus_tag="NBI74_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1930873.1"
                     /translation="MAIIIGLEFAQLPMSFGAKYE"
     gene            14247..14591
                     /gene="erpA"
                     /locus_tag="NBI74_000498"
     CDS             14247..14591
                     /gene="erpA"
                     /locus_tag="NBI74_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HCD1930874.1"
                     /translation="MSDDVALPLEFTDAAANKVKSLIADEDNPNLKLRVYITGGGCSG
                     FQYGFTFDDQVNEGDMTIEKQGVGLVVDPMSLQYLVGGSVDYTEGLEGSRFIVTNPNA
                     KSTCGCGSSFSI"
     gene            complement(14638..15261)
                     /locus_tag="NBI74_000499"
     CDS             complement(14638..15261)
                     /locus_tag="NBI74_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRIC cation channel family protein"
                     /protein_id="HCD1930875.1"
                     /translation="MLVYWLDIVGTAVFAISGVLLAGKLRMDPFGVLVLGVVTAVGGG
                     TIRDMALDHGPVFWVKDPTDLVVAMVTSMLTIVLVRQPRRLPKWMLPVLDAVGLAVFV
                     GIGVNKAFNAEAGPLIAVCMGVITGVGGGIIRDVLAREIPMILRTEIYATACIIGGIV
                     HATAYYTFSVPLETASMMGMVVTLLIRLAAIRWHLKLPTFALDENGR"
     gene            complement(15299..16099)
                     /gene="btuF"
                     /locus_tag="NBI74_000500"
     CDS             complement(15299..16099)
                     /gene="btuF"
                     /locus_tag="NBI74_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter substrate-binding
                     protein BtuF"
                     /protein_id="HCD1930876.1"
                     /translation="MAKSLFRALVALSFLAPLWLNAAPRVITLSPANTELAFAAGITP
                     VGVSSYSDYPPQAQKIEQVSTWQGMNLERIVALKPDLVIAWRGGNAERQVDQLASLGI
                     KVMWVDATSIEQIANALRQLAPWSPQPDKAEQAAQSLLDQYAQLKAQYADKPKKRVFL
                     QFGINPPFTSGKESIQNQVLEVCGGENIFKDSRVPWPQVSREQVLARSPQAIVITGGP
                     DQIPKIKQYWGEQLKIPVIPLTSDWFERASPRIILAAQQLCNALSQVD"
     gene            complement(16092..16790)
                     /gene="mtnN"
                     /locus_tag="NBI74_000501"
     CDS             complement(16092..16790)
                     /gene="mtnN"
                     /locus_tag="NBI74_000501"
                     /EC_number="3.2.2.16"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/S-adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HCD1930877.1"
                     /translation="MKIGIIGAMEEEVTLLRDKIENRQTISLGGCEIYTGQLNGTEVA
                     LLKSGIGKVAAALGATLLLEHCKPDVIINTGSAGGLAPTLKVGDIVVSDEARYHDADV
                     TAFGYEYGQLPGCPAGFKADDKLIAAAEACIAELNLNAVRGLIVSGDAFINGSVGLAK
                     IRHNFPQAIAVEMEATAIAHVCHNFNVPFVVVRAISDVADQQSHLSFDEFLAVAAKQS
                     SLMVESLVQKLAHG"
     gene            16874..18391
                     /gene="dgt"
                     /locus_tag="NBI74_000502"
     CDS             16874..18391
                     /gene="dgt"
                     /locus_tag="NBI74_000502"
                     /EC_number="3.1.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dGTPase"
                     /protein_id="HCD1930878.1"
                     /translation="MAQIDFRKKINWHRRYRSPQGVKTEHEILRIFESDRGRIINSPA
                     IRRLQQKTQVFPLERNAAVRTRLTHSMEVQQVGRYIAKEILSRLKELKLLETYGLDEL
                     TGPFESIVEMSCLMHDIGNPPFGHFGEAAINDWFRQRLYPEDAESQPLTDDRCSVAAL
                     RLRDGEEPLNALRRKIRQDLCHFEGNAQGIRLVHTLMRMNLTWAQVGGILKYTRPAWW
                     RGETPETHHYLMKKPGYYLSEEAYIARLRKELNLALYSRFPLTWIMEAADDISYCVAD
                     LEDAVEKRIFTVEQLYHHLHEAWGQHEKGSLFSLVVENAWEKSRSNSLSRSTEDQFFM
                     YLRVNTLNKLVPYAAQRFIDNLPAIFAGTFNHALLEDASECSDLLKLYKNVAVKHVFS
                     HPDVEQLELQGYRVISGLLEIYRPLLNLPLSDFTELVEKERVKRFPIETRLFHKLSTR
                     HRLAYVEAVSKLPSDSPEFPLWEYYYRCRLLQDYISGMTDLYAWDEYRRLMAVEQ"
     gene            18521..19945
                     /gene="degP"
                     /locus_tag="NBI74_000503"
     CDS             18521..19945
                     /gene="degP"
                     /locus_tag="NBI74_000503"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegP"
                     /protein_id="HCD1930879.1"
                     /translation="MKKTTLALSALALSLGLALSPLSATAAETSSATTAQQMPSLAPM
                     LEKVMPSVVSINVEGSTTVNTPRMPRNFQQFFGDDSPFCQEGSPFQSSPFCQGGPGGN
                     GGGQQQKFMALGSGVIIDADKGYVVTNNHVVDNATVIKVQLSDGRKFDAKMVGKDPRS
                     DIALIQIQNPKNLTAIKMADSDALRVGDYTVAIGNPFGLGETVTSGIVSALGRSGLNA
                     ENYENFIQTDAAINRGNSGGALVNLNGELIGINTAILAPDGGNIGIGFAIPSNMVKNL
                     TSQMVEYGQVKRGELGIMGTELNSDLAKAMKVDAQRGAFVSQVLPNSSAAKAGIKAGD
                     VITSLNGKPISSFAALRAQVGTMPVGSKLTLGLLRDGKQVNVNLELQQSSQNQVDSST
                     IFNGIEGAEMSNKGKDQGVVVNNVKTGTPAAQIGLKKGDVIIGANQQAVKNIAELRKV
                     LDSKPSVLALNIQRGDSTIYLLMQ"
     gene            20100..21257
                     /gene="cdaR"
                     /locus_tag="NBI74_000504"
     CDS             20100..21257
                     /gene="cdaR"
                     /locus_tag="NBI74_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CdaR"
                     /protein_id="HCD1930880.1"
                     /translation="MAGWHLDTKMAQDIVARTMRIIDTNINVMDARGRIIGSGDRERI
                     GELHEGALLVLSQGRVVDIDDAVARHLHGVRQGINLPLRLEGEIVGVIGLTGEPENLR
                     KYGELVCMTAEMMLEQSRLMHLLAQDSRLREELVMNLIQAEENTPALTEWAQRLGIDL
                     NQPRVVAIVEVDSGQLGVDSAMAELQQLQNALTTPERNNLVAIVSLTEMVVLKPALNS
                     FGRWDAEDHRKRVEQLITRMKEYGQLRFRVSLGNYFTGPGSIARSYRTAKTTMVVGKQ
                     RMPESRCYFYQDLMLPVLLDSLRGDWQANELARPLARLKAMDNNGLLRRTLAAWFRHN
                     VQPLATSKALFIHRNTLEYRLNRISELTGLDLGNFDDRLLLYVALQLDEER"
     gene            complement(21311..21697)
                     /locus_tag="NBI74_000505"
     CDS             complement(21311..21697)
                     /locus_tag="NBI74_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000272186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3461 family protein"
                     /protein_id="HCD1930881.1"
                     /translation="MYDNLKSLGITNPEEIDRYSLRQEANNDILKIYFQKDKGEFFAK
                     SVKFKYPRQRKTVVADGVGQGYKEVQEISPNLRYIIDELDQICQRDRSEVDLKRKILD
                     DLRHLESVVTNKISEIEADLEKLTRK"
     gene            complement(22007..22831)
                     /gene="dapD"
                     /locus_tag="NBI74_000506"
     CDS             complement(22007..22831)
                     /gene="dapD"
                     /locus_tag="NBI74_000506"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HCD1930882.1"
                     /translation="MQQLQNIIETAFERRAEITPANADTVTREAVNQVIALLDSGALR
                     VAEKIDGQWVTHQWLKKAVLLSFRINDNQVIEGAESRYFDKVPMKFANYDEARFQKEG
                     FRVVPPAAVRQGAFIARNTVLMPSYVNIGAYVDEGTMVDTWATVGSCAQIGKNVHLSG
                     GVGIGGVLEPLQANPTIIEDNCFIGARSEVVEGVIVEEGSVISMGVYIGQSTRIYDRE
                     TGEIHYGRVPAGSVVVSGNLPSKDGKYSLYCAVIVKKVDAKTRGKVGINELLRTID"
     gene            complement(22862..25534)
                     /gene="glnD"
                     /locus_tag="NBI74_000507"
     CDS             complement(22862..25534)
                     /gene="glnD"
                     /locus_tag="NBI74_000507"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     uridylyltransferase/uridylyl-removing protein GlnD"
                     /protein_id="HCD1930883.1"
                     /translation="MNTLPEQYANTALPTLHGQPQNPCAWPRDELTVGGIKAHIDTFQ
                     RWLGDAFDNGISAEQLIEARTEFIDQLLQRLWIEAGFSQIADLALVAVGGYGRGELHP
                     LSDIDLLILSRKKLPDDQAQKVGELLTLLWDVKLEVGHSVRTLEECMLEGLSDLTVAT
                     NLIESRLLIGDVALFLELQKHIFSEGFWPSDKFYAAKVEEQNQRHQRYHGTSYNLEPD
                     IKSSPGGLRDIHTLQWVARRHFGATSLDEMVGFGFLTSAERAELNECLHILWRIRFAL
                     HLVVSRYDNRLLFDRQLSVAQRLNYSGEGNEPVERMMKDYFRVTRRVSELNQMLLQLF
                     DEAILALPADEKPRPIDDEFQLRGTLIDLRDETLFMRQPEAILRMFYTMVRNSAITGI
                     YSTTLRQLRHARRHLQQPLCNIPEARKLFLSILRHPGAVRRGLLPMHRHSVLGAYMPQ
                     WSHIVGQMQFDLFHAYTVDEHTIRVMLKLESFASEETRQRHPLCVDVWPRLPSTELIF
                     IAALFHDIAKGRGGDHSILGAQDVVHFAELHGLNSRETQLVAWLVRQHLLMSVTAQRR
                     DIQDPEVIKQFAEEVQTENRLRYLVCLTVADICATNETLWNSWKQSLLRELYFATEKQ
                     LRRGMQNTPDMRERVRHHQLQALALLRMDNIDEEALHQIWSRCRANYFVRHSPNQLAW
                     HARHLLQHDLSKPLVLLSPQATRGGTEIFIWSPDRPYLFAAVCAELDRRNLSVHDAQI
                     FTTRDGMAMDTFIVLEPDGSPLSADRHEVIRFGLEQVLTQSSWQPPQPRRQPAKLRHF
                     TVETEVTFLPTHTDRKSFLELIALDQPGLLARVGKIFADLGISLHGARITTIGERVED
                     LFIIATADRRALNNELQQEVHQRLTEALNPNDKG"
     gene            complement(25596..26390)
                     /gene="map"
                     /locus_tag="NBI74_000508"
     CDS             complement(25596..26390)
                     /gene="map"
                     /locus_tag="NBI74_000508"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HCD1930884.1"
                     /translation="MAISIKTPEDIEKMRVAGRLAAEVLEMIEPYVKPGVSTGELDRI
                     CNDYIVNEQHAVSACLGYHGYPKSVCISINEVVCHGIPDDAKLLKDGDIVNIDVTVIK
                     DGFHGDTSKMFIVGKPTIMGERLCRITQESLYLALRMVKPGINLREIGAAIQKFVEAE
                     GFSVVREYCGHGIGRGFHEEPQVLHYDSRETNVVLKPGMTFTIEPMVNAGKKEIRTMK
                     DGWTVKTKDRSLSAQYEHTIVVTDNGCEILTLRKDDTIPAIISHDE"
     gene            26758..27483
                     /gene="rpsB"
                     /locus_tag="NBI74_000509"
     CDS             26758..27483
                     /gene="rpsB"
                     /locus_tag="NBI74_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HCD1930885.1"
                     /translation="MATVSMRDMLKAGVHFGHQTRYWNPKMKPFIFGARNKVHIINLE
                     KTVPMFNEALAELNKIASRKGKILFVGTKRAASEAVKDAALSCDQFFVNHRWLGGMLT
                     NWKTVRQSIKRLKDLETQSQDGTFDKLTKKEALMRTRELEKLENSLGGIKDMGGLPDA
                     LFVIDADHEHIAIKEANNLGIPVFAIVDTNSDPDGVDFVIPGNDDAIRAVTLYLGAVA
                     ATVREGRSQDLASQAEESFVEAE"
     gene            27618..28469
                     /gene="tsf"
                     /locus_tag="NBI74_000510"
     CDS             27618..28469
                     /gene="tsf"
                     /locus_tag="NBI74_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HCD1930886.1"
                     /translation="MAEITASLVKELRERTGAGMMDCKKALTEANGDIELAIENMRKS
                     GAIKAAKKAGNVAADGVIKTKIDGNYGIILEVNCQTDFVAKDAGFQAFADKVLDAAVA
                     GKITDVEVLKAQFEEERVALVAKIGENINIRRVAALEGDVLGSYQHGARIGVLVAAKG
                     ADEELVKHIAMHVAASKPEFIKPEDVSAEVVEKEYQVQLDIAMQSGKPKEIAEKMVEG
                     RMKKFTGEVSLTGQPFVMEPSKTVGQLLKEHNAEVTGFIRFEVGEGIEKVETDFAAEV
                     AAMSKQS"
     gene            28616..29341
                     /gene="pyrH"
                     /locus_tag="NBI74_000511"
     CDS             28616..29341
                     /gene="pyrH"
                     /locus_tag="NBI74_000511"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000224576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HCD1930887.1"
                     /translation="MATNAKPVYKRILLKLSGEALQGTEGFGIDASILDRMAQEIKEL
                     VELGIQVGVVIGGGNLFRGAGLAKAGMNRVVGDHMGMLATVMNGLAMRDALHRAYVNA
                     RLMSAIPLNGVCDSYSWAEAISLLRNNRVVILSAGTGNPFFTTDSAACLRGIEIEADV
                     VLKATKVDGVFTADPAKDPTATMYEQLTYSEVLEKELKVMDLAAFTLARDHKLPIRVF
                     NMNKPGALRRVVMGEKEGTLITE"
     gene            29491..30048
                     /gene="frr"
                     /locus_tag="NBI74_000512"
     CDS             29491..30048
                     /gene="frr"
                     /locus_tag="NBI74_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HCD1930888.1"
                     /translation="MISDIRKDAEVRMDKCVEAFKTQISKIRTGRASPSLLDGIVVEY
                     YGTPTPLRQLASVTVEDSRTLKINVFDRSMSPAVEKAIMASDLGLNPNSAGSDIRVPL
                     PPLTEERRKDLTKIVRGEAEQARVAVRNVRRDANDKVKALLKDKEISEDDDRRSQDDV
                     QKLTDAAIKKIEAALADKEAELMQF"
     gene            30140..31336
                     /gene="ispC"
                     /locus_tag="NBI74_000513"
     CDS             30140..31336
                     /gene="ispC"
                     /locus_tag="NBI74_000513"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HCD1930889.1"
                     /translation="MKQLTILGSTGSIGCSTLDVVRHNPEHFRVVALVAGKNVTRMVE
                     QCLEFSPRYAVMDDEASAKLLKTMLQQQGSRTEVLSGQQAACDMAALKDVDQVMAAIV
                     GAAGLLPTLAAIRAGKTILLANKESLVTCGRLFMDAVKQSKAQLLPVDSEHNAIFQSL
                     PQPIQHNLGYADLEQNGVVSILLTGSGGPFRETPLRDLATMTPDQACRHPNWSMGRKI
                     SVDSATMMNKGLEYIEARWLFNASASQMEVLIHPQSVIHSMVRYQDGSVLAQLGEPDM
                     RTPIAHTMAWPNRVNSGVKPLDFCKLSALTFAAPDYDRYPCLKLAMEAFEQGQAATTA
                     LNAANEITVAAFLAQQIRFTDIAALNLSVLEKMDMREPQCVDDVLSVDANAREVARKE
                     VMRLAS"
     gene            31525..32283
                     /gene="ispU"
                     /locus_tag="NBI74_000514"
                     /gene_synonym="uppS"
     CDS             31525..32283
                     /gene="ispU"
                     /locus_tag="NBI74_000514"
                     /gene_synonym="uppS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl-diphosphate-specific
                     ditrans,polycis-undecaprenyl-diphosphate synthase"
                     /protein_id="HCD1930890.1"
                     /translation="MLSATQPLSEKLPAHGCRHVAIIMDGNGRWAKKQGKIRAFGHKA
                     GAKSVRRAVSFAANNGIEALTLYAFSSENWNRPAQEVSALMELFVWALDSEVKSLHRH
                     NVRLRIIGDTSRFNSRLQERIRKSEALTAGNTGLTLNIAANYGGRWDIVQGVRQLAEK
                     VQQGNLQPDQIDEEMLNQHVCMHELAPVDLVIRTGGEHRISNFLLWQIAYAELYFTDV
                     LWPDFDEQDFEGALNAFANRERRFGGTEPGDETA"
     gene            32296..33153
                     /gene="cdsA"
                     /locus_tag="NBI74_000515"
     CDS             32296..33153
                     /gene="cdsA"
                     /locus_tag="NBI74_000515"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HCD1930891.1"
                     /translation="MLKYRLISAFVLIPVVIAALFLLPPVGFAIVTLVVCMLAAWEWG
                     QLSGFTTRSQRVWLAVLCGLLLALMLFLLPEYHRNIHQPLVEISLWASLGWWIVALLL
                     VLFYPGSAAIWRNSKTLRIIFGVLTIVPFFWGMLALRAWHYDENHYSGAIWLLYVMIL
                     VWGADSGAYMFGKLFGKHKLAPKVSPGKTWQGFIGGLVTAAVISWGYGMWVNLDVAPV
                     TLLICSIVAALASVLGDLTESMFKREAGIKDSGHLIPGHGGILDRIDSLTAAVPVFAC
                     LLLLVFRTL"
     gene            33165..34517
                     /gene="rseP"
                     /locus_tag="NBI74_000516"
     CDS             33165..34517
                     /gene="rseP"
                     /locus_tag="NBI74_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma E protease regulator RseP"
                     /protein_id="HCD1930892.1"
                     /translation="MLSFLWDLASFIVALGVLITVHEFGHFWVARRCGVRVERFSIGF
                     GKALWRRTDKLGTEYVIALIPLGGYVKMLDERAEPVVPELRHHAFNNKSVGQRAAIIA
                     AGPVANFIFAIFAYWLVFIIGVPGVRPVVGEIAANSIAAEAQIAPGTELKAVDGIETP
                     DWDAVRLQLVDKIGDESTTITVAPFGSDQRRDVKLDLRHWAFEPDKEDPVSSLGIRPR
                     GPQIEPVLENVQPNSAASKAGLQAGDRIVKVDGQPLTQWVTFVMLVRDNPGKSLALEI
                     ERQGSPLSLTLIPESKPGNGKAIGFVGIEPKVIPLPDEYKVVRQYGPFNAIVEATDKT
                     WQLMKLTVSMLGKLITGDVKLNNLSGPISIAKGAGMTAELGVVYYLPFLALISVNLGI
                     INLFPLPVLDGGHLLFLAIEKIKGGPVSERVQDFCYRIGSILLVLLMGLALFNDFSRL
                     "
     gene            34547..36979
                     /gene="bamA"
                     /locus_tag="NBI74_000517"
     CDS             34547..36979
                     /gene="bamA"
                     /locus_tag="NBI74_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HCD1930893.1"
                     /translation="MAMKKLLIASLLFSSATVYGAEGFVVKDIHFEGLQRVAVGAALL
                     SMPVRTGDTVNDEDISNTIRALFATGNFEDVRVLRDGDTLLVQVKERPTIASITFSGN
                     KSVKDDMLKQNLEASGVRVGESLDRTTIADIEKGLEDFYYSVGKYSASVKAVVTPLPR
                     NRVDLKLVFQEGVSAEIQQINIVGNHAFTTDELISHFQLRDEVPWWNVVGDRKYQKQK
                     LAGDLETLRSYYLDRGYARFNIDSTQVSLTPDKKGIYVTVNITEGDQYKLSGVEVSGN
                     LAGHSAEIEQLTKIEPGELYNGTKVTKMEDDIKKLLGRYGYAYPRVQSMPEINDADKT
                     VKLRVNVDAGNRFYVRKIRFEGNDTSKDAVLRREMRQMEGAWLGSDLVDQGKERLNRL
                     GFFETVDTDTQRVPGSPDQVDVVYKVKERNTGSFNFGIGYGTESGVSFQAGVQQDNWL
                     GTGYAVGINGTKNDYQTYAELSVTNPYFTVDGVSLGGRLFYNDFQADDADLSDYTNKS
                     YGTDVTLGFPINEYNSLRAGLGYVHNSLSNMQPQVAMWRYLYSMGEHPSTSDQDNSFK
                     TDDFTFNYGWTYNKLDRGYFPTDGSRVNLTGKVTIPGSDNEYYKVTLDTATYVPIDDD
                     HKWVVLGRTRWGYGDGLGGKEMPFYENFYAGGSSTVRGFQSNTIGPKAVYFPHQASNY
                     DPDYDYECATQDGAKDLCKSDDAVGGNAMAVASLEFITPTPFISDKYANSVRTSFFWD
                     MGTVWDTNWDSSQYSGYPDYSDPSNIRMSAGIALQWMSPLGPLVFSYAQPFKKYDGDK
                     AEQFQFNIGKTW"
     gene            37101..37586
                     /gene="skp"
                     /locus_tag="NBI74_000518"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
     CDS             37101..37586
                     /gene="skp"
                     /locus_tag="NBI74_000518"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone Skp"
                     /protein_id="HCD1930894.1"
                     /translation="MKKWLLAAGLGLALATSAQAADKIAIVNMGSLFQQVAQKTGVSN
                     TLENEFKGRASELQRMETDLQAKMKKLQSMKAGSDRTKLEKDVMAQRQTFAQKAQAFE
                     QDRARRSNEERGKLVTRIQTAVKSVANSQDIDLVVDANAVAYNSSDVKDITADVLKQV
                     K"
     gene            37590..38615
                     /gene="lpxD"
                     /locus_tag="NBI74_000519"
     CDS             37590..38615
                     /gene="lpxD"
                     /locus_tag="NBI74_000519"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HCD1930895.1"
                     /translation="MPSIRLADLAQQLDAELHGDGDIVITGVASMQSAQTGHITFMVN
                     PKYREHLGLCQASAVVMTQDDLPFAKSAALVVKNPYLTYARMAQILDTTPQPAQNIAP
                     SAVIDATAKLGNNVSIGANAVIESGVELGDNVIIGAGCFVGKNSKIGAGSRLWANVTI
                     YHEIQIGQNCLIQSGTVVGADGFGYANDRGNWVKIPQIGRVIIGDRVEIGACTTIDRG
                     ALDDTVIGNGVIIDNQCQIAHNVVIGDNTAVAGGVIMAGSLKIGRYCMIGGASVINGH
                     MEICDKVTVTGMGMVMRPITEPGVYSSGIPLQPNKVWRKTAALVMNIDDMSKRLKSLE
                     RKVNQQD"
     gene            38720..39175
                     /gene="fabZ"
                     /locus_tag="NBI74_000520"
     CDS             38720..39175
                     /gene="fabZ"
                     /locus_tag="NBI74_000520"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HCD1930896.1"
                     /translation="MTTNTHTLQIEEILELLPHRFPFLLVDRVLDFEEGRFLRAVKNV
                     SVNEPFFQGHFPGKPIFPGVLILEAMAQATGILAFKSVGKLEPGELYYFAGIDEARFK
                     RPVVPGDQMIMEVTFEKTRRGLTRFKGVALVDGKVVCEATMMCARSREA"
     gene            39179..39967
                     /gene="lpxA"
                     /locus_tag="NBI74_000521"
     CDS             39179..39967
                     /gene="lpxA"
                     /locus_tag="NBI74_000521"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HCD1930897.1"
                     /translation="MIDKSAFVHPTAIVEEGASIGANAHIGPFCIVGPHVEIGEGTVL
                     KSHVVVNGHTKIGRDNEIYQFASIGEVNQDLKYAGEPTRVEIGDRNRIRESVTIHRGT
                     VQGGGLTKVGSDNLLMINAHIAHDCTVGNRCILANNATLAGHVSVDDFAIIGGMTAVH
                     QFCIIGAHVMVGGCSGVAQDVPPYVIAQGNHATPFGVNIEGLKRRGFSREAITAIRNA
                     YKLIYRSGKTLDEVKPEIAELAETYPEVKAFTDFFARSTRGLIR"
     gene            39967..41115
                     /gene="lpxB"
                     /locus_tag="NBI74_000522"
     CDS             39967..41115
                     /gene="lpxB"
                     /locus_tag="NBI74_000522"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HCD1930898.1"
                     /translation="MTEQRPLTIALVAGETSGDILGAGLIRALKERVPNARFVGVAGP
                     RMQAEGCEAWYEMEELAVMGIVEVLGRLRRLLHIRADLTKRFGELKPDVFVGIDAPDF
                     NITLEGNLKKQGIKTIHYVSPSVWAWRQKRVFKIGRATDLVLAFLPFEKAFYDKYNVP
                     CRFIGHTMADAMPLDPDKNGARDVLGIPYDAHCLALLPGSRGAEVEMLSADFLKTAQL
                     LRQTYPDLEIVVPLVNAKRREQFERIKAAVAPDLSVHLLDGMGREAMVASDAALLASG
                     TAALECMLAKCPMVVGYRMKPFTFWLAKRLVKTDYVSLPNLLAGRELVKELLQEECEP
                     QKLAAALLPLLANGKTSHAMHDTFRELHQQIRCNADEQAAQAVLELAQ"
     gene            41112..41708
                     /gene="rnhB"
                     /locus_tag="NBI74_000523"
     CDS             41112..41708
                     /gene="rnhB"
                     /locus_tag="NBI74_000523"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HCD1930899.1"
                     /translation="MIEFVYPHTQLVAGVDEVGRGPLVGAVVTAAVILDPARPIAGLN
                     DSKKLSEKRRLALCEEIKEKALSWSLGRAEPHEIDELNILHATMLAMQRAVAGLHIAP
                     EYVLIDGNRCPKLPMPSMAVVKGDSRVPEISAASILAKVTRDAEMAALDIVFPQYGFA
                     QHKGYPTAFHLEKLAEHGATEHHRRSFGPVKRALGLAS"
     gene            41745..45227
                     /gene="dnaE"
                     /locus_tag="NBI74_000524"
     CDS             41745..45227
                     /gene="dnaE"
                     /locus_tag="NBI74_000524"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HCD1930900.1"
                     /translation="MSEPRFVHLRVHSDYSMIDGLAKTAPLVKKAAALGMPALAITDF
                     TNLCGLVKFYGAGHGAGIKPIVGADFNVQCDLLGDELTHLTVLAANNTGYQNLTLLIS
                     KAYQRGYGAAGPIIDRDWLIELNEGLILLSGGRMGDVGRSLLRGNSALVDECVAFYEE
                     HFPDRYFLELIRTGRPDEESYLHAAVELAESRGLPVVATNDVRFIDSSDFDAHEIRVA
                     IHDGFTLDDPKRPRNYSPQQYMRSEEEMCELFADIPEALANTVEIAKRCNVTVRLGEY
                     FLPQFPTGDMSTEDYLVKRAKEGLEERLAFLFPDEEERLKRRPEYDERLDTELQVINQ
                     MGFPGYFLIVMEFIQWSKDNGVPVGPGRGSGAGSLVAYALKITDLDPLEFDLLFERFL
                     NPERVSMPDFDVDFCMEKRDQVIEHVADMYGRDAVSQIITFGTMAAKAVIRDVGRVLG
                     HPYGFVDRISKLIPPDPGMTLAKAFEAEPQLPEIYEADEEVKALIDMARKLEGVTRNA
                     GKHAGGVVIAPTKITDFAPLYCDEEGKHPVTQFDKSDVEYAGLVKFDFLGLRTLTIIN
                     WALEMINKRRAKNGEPPLDIAAIPLDDKKSFDMLQRSETTAVFQLESRGMKDLIKRLQ
                     PDCFEDMIALVALFRPGPLQSGMVDNFIDRKHGREEISYPDVQWQHESLKPVLEPTYG
                     IILYQEQVMQIAQVLSGYTLGGADMLRRAMGKKKPEEMAKQRSVFAEGAEKNGINAEL
                     AMKIFDLVEKFAGYGFNKSHSAAYALVSYQTLWLKAHYPAEFMAAVMTADMDNTEKVV
                     GLVDECWRMGLKILPPDINSGLYHFHVNDDGEIVYGIGAIKGVGEGPIEAIIEARNKG
                     GYFRELFDLCARTDTKKLNRRVLEKLIMSGAFDRLGPHRAALMNSLGDALKAADQHAK
                     AEAIGQADMFGVLAEEPEQIEQSYASCQPWPEQVVLDGERETLGLYLTGHPINQYLKE
                     IERYVGGVRLKDMHPTERGKVITAAGLVVAARVMVTKRGNRIGICTLDDRSGRLEVML
                     FTDALDKYQQLLEKDRILIVSGQVSFDDFSGGLKMTAREVMDIDEAREKYARGLAISL
                     TDRQIDDQLLNRLRQSLEPHRSGTIPVHLYYQRADARARLRFGATWRVSPSDRLLNDL
                     RGLIGSEQVELEFD"
     gene            45240..46199
                     /gene="accA"
                     /locus_tag="NBI74_000525"
     CDS             45240..46199
                     /gene="accA"
                     /locus_tag="NBI74_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HCD1930901.1"
                     /translation="MSLNFLDFEQPIAELEAKIDSLTAVSRQDEKLDINIDEEVHRLR
                     EKSVELTRKIFADLGAWQIAQLARHPQRPYTLDYVRLAFDEFDELAGDRAYADDKAIV
                     GGIARLDGRPVMIIGHQKGRETKEKIRRNFGMPAPEGYRKALRLMQMAERFKMPIITF
                     IDTPGAYPGVGAEERGQSEAIARNLREMSRLGVPVVCTVIGEGGSGGALAIGVGDKVN
                     MLQYSTYSVISPEGCASILWKSADKAPLAAEAMGIIAPRLKELKLIDSIIPEPLGGAH
                     RNPEAMAASLKAQLLADLADLDVLSTEDLKNRRYQRLMSYGYA"
     gene            46297..48438
                     /gene="ldcC"
                     /locus_tag="NBI74_000526"
     CDS             46297..48438
                     /gene="ldcC"
                     /locus_tag="NBI74_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase LdcC"
                     /protein_id="HCD1930902.1"
                     /translation="MNIIAIMGPHGVFYKDEPIKELESALVAQGFQIIWPQNSVDLLK
                     FIEHNPRICGVIFDWDEYSLDLCSDINQLNEYLPLYAFINTHSTMDVSVQDMRMALWF
                     FEYALGQAEDIAIRMRQYTNEYLDNITPPFTKALFTYVKERKYTFCTPGHMGGTAYQK
                     SPVGCLFYDFFGGNTLKADVSISVTELGSLLDHTGPHLEAEEYIARTFGAEQSYIVTN
                     GTSTSNKIVGMYAAPSGSTLLIDRNCHKSLAHLLMMNDVVPVWLKPTRNALGILGGIP
                     RGEFTRDSIEEKVAATTQAQWPVHAVITNSTYDGLLYNTDWIKQTLDVPSIHFDSAWV
                     PYTHFHPIYQGKSGMSGERVAGKVIFETQSTHKMLAALSQASLIHIKGEYDEEAFNEA
                     FMMHTTTSPSYPIVASVETAAAMLRGNPGKRLINRSVERALHFRKEVQRLREESDSWF
                     FDIWQPPQVDEAECWHVAPGEQWHGFSDADANHMFLDPVKVTILTPGMDEQGNMSEEG
                     IPAALVAKFLDERGIVVEKTGPYNLLFLFSIGIDKTKAMGLLRGLTEFKRSYDLNLRI
                     KNMLPDLYAEDPDFYRNMRIQDLAQGIHKLIRKHDLPGLMLRAFDTLPEMIMTPHQAW
                     QRQIKGEVETIALEQLVGRVSANMILPYPPGVPLLMPGEMLTEESRTVLDFLLMLCSV
                     GQHYPGFETDIHGAKQDEDGVYRVRVLKMAG"
     gene            48495..48884
                     /locus_tag="NBI74_000527"
     CDS             48495..48884
                     /locus_tag="NBI74_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCD1930903.1"
                     /translation="MLGLKQVHHIAIIATDYAMSKAFYCDILGFTLQSEVYREARDSW
                     KGDLALNGQYVIELFSFPFPPERPSRPEACGLRHLAFSVDDIDAAVAHLESHNVKCEA
                     IRVDPYTQKRFTFFNDPDGLPLELYEQ"
     gene            48949..50262
                     /gene="tilS"
                     /locus_tag="NBI74_000528"
     CDS             48949..50262
                     /gene="tilS"
                     /locus_tag="NBI74_000528"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000176585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HCD1930904.1"
                     /translation="MTLTLNRQLLSSRQILVAFSGGLDSTVLLHQLVQWRTENPGVTL
                     RAIHVHHGLSANADAWVKHCENICQQWQVPLVVERVQLAQEGLGIEAQARQARYQAFA
                     RTLLPGEVLVTAQHLDDQCETFLLALKRGSGPAGLSAMAEVSEFAGTRLIRPLLARTR
                     GELEQWARQYDLRWIEDESNQDDSYDRNFLRLRVVPLLQQRWPHFAEATARSAALCAE
                     QESLLDELLADDLAHCQTQQGTLKIAPMLAMSDARRAAIIRRWLAGQNAPMPSREALV
                     RIWQEVALAREDASPCLRLGAFEIRRYQSQLWWIKSVAGQSETIVPWQTWLQPLELPA
                     GLGSVQLTAGGDIRPPRADEAVSVRFKAPGLLHIVGRNGGRKLKKIWQELGVPPWLRD
                     TTPLLFYGETLIAAAGVFVTQEGVAVGENGVSFVWQKTLSQVKAG"
     gene            complement(50296..50556)
                     /gene="rof"
                     /locus_tag="NBI74_000529"
     CDS             complement(50296..50556)
                     /gene="rof"
                     /locus_tag="NBI74_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rho-binding antiterminator"
                     /protein_id="HCD1930905.1"
                     /translation="MSMNDTYQPINCDDYDNLELACQHHLMLTLELKDGEKLQAKASD
                     LVSRKNVEYLVVEAAGATRELRLDKITSFSHPEIGTVVVSES"
     gene            complement(50543..50743)
                     /locus_tag="NBI74_000530"
     CDS             complement(50543..50743)
                     /locus_tag="NBI74_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeP family protein"
                     /protein_id="HCD1930906.1"
                     /translation="MEKYCELIRKRYAEIASGDLGYVPDALGCVLKVLNEMAADDALS
                     EAVREKAAYAAANLLVSDYVNE"
     gene            50909..51454
                     /locus_tag="NBI74_000531"
     CDS             50909..51454
                     /locus_tag="NBI74_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeQ family protein"
                     /protein_id="HCD1930907.1"
                     /translation="MALKATIYKATVNVADLDRNQFLDASLTLARHPSETQERMMLRL
                     LAWLKYADERLQFTRGLCADDEPEAWLRNDHLGIDLWIELGLPDERRIKKACTQAAEV
                     ALFAYNSRAAQIWWQQNQSKCAQFANLSVWYLDDEQLAKVSAFADRTMTLQATIQDGV
                     IWLSDDKNNLEVNLTVWQQPS"
     gene            51451..51873
                     /gene="arfB"
                     /locus_tag="NBI74_000532"
     CDS             51451..51873
                     /gene="arfB"
                     /locus_tag="NBI74_000532"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HCD1930908.1"
                     /translation="MIVISRHVAIPDGEFEITAIRAQGAGGQHVNKTSTAIHLRFDIR
                     ASSLPEYYKERLLAASHHLISSDGVIVIKAQEYRSQELNREAALARLVAVIKDLTTEQ
                     KARRPTRPTRASKERRLASKAQKSSVKAMRGKVRSGRE"
     gene            51887..52597
                     /gene="nlpE"
                     /locus_tag="NBI74_000533"
                     /gene_synonym="cutF"
     CDS             51887..52597
                     /gene="nlpE"
                     /locus_tag="NBI74_000533"
                     /gene_synonym="cutF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308221.1"
                     /note="NlpE, an outer membrane lipoprotein, interacts
                     directly with CpxA, the sensor histidine kinase of the Cpx
                     system for response to envelope stress; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response activation lipoprotein
                     NlpE"
                     /protein_id="HCD1930909.1"
                     /translation="MVKKAIVTAMAVISLFTLMGCNNRAEVDTLSPAQAAELKPMPQS
                     WRGVLPCADCEGIETSLFLEKDGTWVMNERYLGAREEPSSFASYGTWARTADKLVLTD
                     SKGEKSYYRAKGDALEMLDREGNPIESQFNYTLEPAKSSMPMTPMTLRGMYFYMADAA
                     TFTDCATGKRFMVANNAELERGYLAARGNGEKPVLLSVEGHFTLEANPDTGAPTKVLA
                     PDTAGKFYPNQDCSSLGQ"
     gene            complement(52753..53577)
                     /locus_tag="NBI74_000534"
     CDS             complement(52753..53577)
                     /locus_tag="NBI74_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeF family permuted papain-like enzyme"
                     /protein_id="HCD1930910.1"
                     /translation="MDKPKAYCRLLLPCFLLLSACTVDISQPDSSATAVNAEAKTWAV
                     KFQHQSSFTEQSIKEITEPDLKPGDLLFSSSLGVTSFGIRVFSTSSVSHVAIYLGNNN
                     VAEATGAGVQIVSLKKAMKHSDKLFVLRVPDLTPQQATEITAFANKIKDSGYNYRGIV
                     EFIPFMVTRQMCSLNPFSEDFRQQCVSGLAKAQLSNVGEGDKKSWFCSEFVTDAFAKA
                     GHPLTLAQSGWISPADLMHMRSGDVSAFKPETQLQYIGHLKPGIYIKASRFVGLTQ"
     gene            complement(53630..55348)
                     /gene="proS"
                     /locus_tag="NBI74_000535"
     CDS             complement(53630..55348)
                     /gene="proS"
                     /locus_tag="NBI74_000535"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HCD1930911.1"
                     /translation="MRTSQYLLSTLKETPADAEVISHQLMLRAGMIRKLASGLYTWLP
                     TGVRVLKKVENIVREEMNNAGAIEVLMPVVQPADLWQESGRWEQYGPELLRFVDRGER
                     PFVLGPTHEEVITDLIRNELSSYKQLPLNFYQIQTKFRDEVRPRFGVMRSREFLMKDA
                     YSFHTSQESLQETYDAMYAAYSKIFSRMGLDFRAVQADTGSIGGSASHEFQVLAQSGE
                     DDVVFSDTSDYAANIELAEAIAPKEPRAAATQEMTLVDTPNAKTIAELVEQFNLPIEK
                     TVKTLLVKAVEGSSFPLVALLVRGDHELNEVKAEKLPQVASPLTFATEEEIRAVVKAG
                     PGSLGPVNMPIPVVIDRTVAAMSDFAAGANIDGKHYFGINWDRDVATPEIADIRNVVA
                     GDPSPDGQGTLLIKRGIEVGHIFQLGTKYSEALKASVQGEDGRNQILTMGCYGIGVTR
                     VVAAAIEQNYDERGIVWPDAIAPFQVAILPMNMHKSFRVQELAEKLYSELRAQGIEVL
                     LDDRKERPGVMFADMELIGIPHTIVLGDRNLDNDDIEYKYRRNGEKQLIKTGDIVDYL
                     VKQIKG"
     gene            complement(55459..56166)
                     /gene="tsaA"
                     /locus_tag="NBI74_000536"
     CDS             complement(55459..56166)
                     /gene="tsaA"
                     /locus_tag="NBI74_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HCD1930912.1"
                     /translation="MSSFQFEQIGVIRSPYKEKFAVPRQPGLVKSANGELHLIAPYNQ
                     ADAVRGLEAFSHLWILFVFHQTMEGGWRPTVRPPRLGGNARMGVFATRSTFRPNPIGM
                     SLVELKEVVCHKDSVILKLGSLDLVDGTPVVDIKPYLPFAESLPDASASYAQSAPAAE
                     MAVCFTAEVEKQLLTLEKRYPQLTLFIREVLAQDPRPAYRKGEETGKTYAVWLHDFNV
                     RWRVTDAGFEVFALEPR"
     gene            complement(56163..56567)
                     /gene="rcsF"
                     /locus_tag="NBI74_000537"
     CDS             complement(56163..56567)
                     /gene="rcsF"
                     /locus_tag="NBI74_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rcs stress response system protein RcsF"
                     /protein_id="HCD1930913.1"
                     /translation="MRALPICLVALMLSGCSMLSRSPVEPVQSTAPQPKAEPAKPKAP
                     RATPVRIYTNAEELVGKPFRDLGEVSGDSCQASNQDSPPSIPTARKRMQINASKMKAN
                     AVLLHSCEVTSGTPGCYRQAVCIGSALNITAK"
     gene            complement(56685..57500)
                     /gene="metQ"
                     /locus_tag="NBI74_000538"
     CDS             complement(56685..57500)
                     /gene="metQ"
                     /locus_tag="NBI74_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter substrate-binding
                     lipoprotein MetQ"
                     /protein_id="HCD1930914.1"
                     /translation="MAFKFKTFAAVGALIGSLALVGCGQDEKDPNHIKVGVIVGAEQQ
                     VAEVAQKVAKDKYGLDVELVTFNDYVLPNEALSKGDIDANAFQHKPYLDQQLKDRGYK
                     LVAVGNTFVYPIAGYSKKIKSLDELQDGSQVAVPNDPTNLGRSLLLLQKVGLIKLKDG
                     VGLLPTVLDVVENPKNLKIVELEAPQLPRSLDDAQIALAVINTTYASQIGLTPAKDGI
                     FVEDKESPYVNLIVTREDNKDAENVKKFVQAYQSDEVYEAANKVFNGGAVKGW"
     gene            complement(57540..58193)
                     /gene="metI"
                     /locus_tag="NBI74_000539"
     CDS             complement(57540..58193)
                     /gene="metI"
                     /locus_tag="NBI74_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease MetI"
                     /protein_id="HCD1930915.1"
                     /translation="MSEPMMWLLVRGVWETLAMTFVSGFFGFVIGLPVGVLLYVTRPG
                     QIIANAKLYRTISAIVNIFRSIPFIILLVWMIPFTRVIVGTSIGLQAAIVPLTVGAAP
                     FIARMVENALLEIPTGLIEASRAMGATPMQIVRKVLLPEALPGLVNAATITLITLVGY
                     SAMGGAVGAGGLGQIGYQYGYIGYNATVMNTVLVLLVILVYLIQFAGDRIVRAVTRK"
     gene            complement(58186..59217)
                     /gene="metN"
                     /locus_tag="NBI74_000540"
     CDS             complement(58186..59217)
                     /gene="metN"
                     /locus_tag="NBI74_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein
                     MetN"
                     /protein_id="HCD1930916.1"
                     /translation="MIKLSNITKVFHQGTRTIQALNNVSLHVPAGQIYGVIGASGAGK
                     STLIRCVNLLERPTEGSVLVDGQELTTLSESELTKARRQIGMIFQHFNLLSSRTVFGN
                     VALPLELDNTPKDEIKRRVTELLSLVGLGDKHDSYPSNLSGGQKQRVAIARALASNPK
                     VLLCDEATSALDPATTRSILELLKDINRRLGLTILLITHEMDVVKRICDCVAVISNGE
                     LIEQDTVSEVFSHPKTPLAQKFIQSTLHLDIPEDYQERLQAEPFTDCVPMLRLEFTGQ
                     SVDAPLLSETARRFNVNNNIISAQMDYAGGVKFGIMLTEMHGTQQDTQAAIAWLQEHH
                     VKVEVLGYV"
     gene            59405..59977
                     /gene="gmhB"
                     /locus_tag="NBI74_000541"
     CDS             59405..59977
                     /gene="gmhB"
                     /locus_tag="NBI74_000541"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HCD1930917.1"
                     /translation="MAKSVPAIFLDRDGTINVDHGYVHEIDNFEFIDGVIDAMRELKK
                     MGFALVVVTNQSGIARGKFTEAQFETLTEWMDWSLADRDVDLDGIYYCPHHPQGSVEE
                     FRQVCDCRKPHPGMFLSARDYLHIDMAASYMVGDKLEDMQAAAAASVGTKVLVRTGKP
                     ITPEAENAADWVLNSLADLPQAIKKQQKPA"
BASE COUNT        14491 a        14471 c        16249 g        14824 t
ORIGIN      
        1 tctaaaaata atattatttg gtggatatat ttctaatatc aatatttatc aagccctgac
       61 cagtcgctct ggaatggaat catgtgtatt acctcataat taagggattt ttatgaataa
      121 aaaattgctt tctctgttag tgtcaggaag tctcatcgct gtagtaagta ccagtgtatt
      181 tgctacagat accggagtgg ttaactttaa cggtaaaatt gttgccgata cttgcgaaat
      241 taatgtaaac gagtctgcca gtacaacagg gacagtcact tttgcagata cttacccagc
      301 ggactattct ggagacggta gtattggaac gtcaaaagat tttaaaattg aattaataaa
      361 atgcgatccg ttggtggaca aactaaacct gacattttct ggaacgacaa cggatgcagg
      421 tttcctgcgt ctggaaaata gcgaagctgg ggctggcagt gcaacaaatg tgggtattac
      481 ggtgacaaat aaaaatggcg gtgctaacga tgtgaaattt gatggttcag ttcccgatgc
      541 atccactgat gttgataatg ccgggagcat tactccaaca atattcaatt acaccgccaa
      601 tgttattcag gttggtaata acgcgccgac tgcgggtaag tatgcctcat ctgcgacgtt
      661 cgaagtattt tatcgctaat attcatagtg tttgaaagga ctgtttgacc acggagggtt
      721 atttatgtca gttttttctc gagtttgttt aggttttacg tttcttctta cggcattgca
      781 agttcaggca agtattgttc ttggcggtac acgtattatc tatccttcaa atcaaaatga
      841 agttcaaatc acattaaaaa ataaagatgc atatgcacgc tatttggtgc aaagctgggt
      901 gagtaatatt gatggctcta aagccccctt tctcattacg cctccagttt ataaacttga
      961 agaaaatcgg caaacattac tccatattgt atttacaggt gataaaaaca aacttcctca
     1021 ggatagggaa agtttgtttc tggcaaacat aaaatctgtt tctgcgatgc cagaagaact
     1081 aaaagataaa aataccttgc aatttgcaat gaaagcccgg cttaaattat tttggcggcc
     1141 agcttctttg gataatagtg atgctctcac tgcatgggaa aaacttaagt tccataaaga
     1201 agcggggaag ctcatagtaa aaaatccaac gcctttttat atttcattca gtgatctgac
     1261 agtatcggga aaaaatattg ttcccaccga gagcaaatct gaacctggcg cattattgat
     1321 gatggtagga ccattttctg agcagcggtt tagtcttcct gtcggagcgg gaagtattgt
     1381 tgaatggtcg gtgataactg actacggttc aagttctggg aaacgacaac aaaagttata
     1441 aatcaccgaa gtgtattact ataaataacc gatctgtatt tataatatta cgggcgcaag
     1501 ggaatttatg ctgctaccat tgcatcgcac atttgtctta actgggataa catttgcatt
     1561 atcggcagtg tattcattga gctatgcacg agatgaattt aaccttcgta tcctggagct
     1621 ggatagccca cttgaaaata cacaagtgct ggaggatttt gttaataata ataacttaac
     1681 tccgggagta tatcttacct ctgttatgtg ggggcaagag tatcttgata agagaaatat
     1741 taccttcatt ctgtcttcgg ataaaaaaag gctcatccct cgttttacaa aggcggatct
     1801 ccgtgagttc ggtcttaaag ttgacgacat tcccgcgtta caagtaatgg atgatgacac
     1861 tgagtttggt gatattgctc agattattga tggcgcacgt tatgatttcc agttagacag
     1921 ccaaacgctg tgtctgcgga tcccgcaaat ctatcagaat gcgcgggccg cagggagtat
     1981 ttcaccgaaa tactggagtg atggtgagag tgcggtttgg ttaagttatt acgccagtgg
     2041 ttcccggcaa aacagtgatg gcgataacct gaattcaaat tggttgaatt taaattcagg
     2101 gattaattta ggcgtatggc ggctacgaaa taataccgtt tacagtgact ccagttggga
     2161 gtcgatcagc accagtttgc aacgcgatat caaagcatta agaagccaga tggaagtcgg
     2221 gcagacgttc acaaatggcg atctttttga cagtgtgcag atgacaggta taaagcttga
     2281 aacggacacc agtatgctgc ctgacagcga acaaggcttt gcgccagtgg ttcgtggtat
     2341 tgccaacagc gatgcacaag ttgtgattaa acaaaacggc tatgttattt accaaacgtg
     2401 ggtatcggca gggccatttg aaattaaaga cttaagccag gtaacggcgg gggccgatct
     2461 ggaagtcacc atcaaagaaa ccaacgggca agaacacagt tttattcagg ccagttcaac
     2521 ggttccaatt ctacagcgag aaggcgcatt aaaatatagt cttgcaactg gaaaataccg
     2581 tgacaacgat aatcatgctg agacacctgt ttttggtgtt gcaaccgcga tatatggctt
     2641 gccctatgga ataacgattt atggcggcat acttggtgcc tcaatttatc attctggtgt
     2701 gacagggatt ggcgctgatt taggccgtct ggggtcggta tctgttgata tcaccgcagc
     2761 agaaacgaag tttgatgatg ggcgtgatga tgctacagga ctgtcatggc gtgcacagta
     2821 tgcgaaagat tttccagaca ctgatacgac agtaactctg gcaagttatc gttattctac
     2881 atcacaattt tacactttcc aggaagcact tgatcaacgt gatacacctg atgacaaggg
     2941 tatttacagt tatcgacaga caaataatcg gcgtaatcgt ttacaaatta atctttcaca
     3001 aaatattggt cgttggggct cggtctacct taacggttat caacaggatt attggggaat
     3061 gcatggggcc gaacgcagta taggaatggg atacagtacg acctggagta acattaattg
     3121 gtctgttaat tacacactaa cgaaaacgcc aggaatggct ggtgagcagc aattttcatt
     3181 aacactcaat attcctctct ctcgctggct gccagattca tgggcgatgt ataacgtaaa
     3241 ccgttccgat aaaagtaata catcacatca attgggaatt ggcggtactg ctctgcaaga
     3301 taacaacctg agttataatt tacagcaaag ttatactgat aataatgtgg gttatgatgc
     3361 cagcatgaat ggacgttatc gtagctcagt gggggaattc ggtctgggat atagctatga
     3421 taaaaatagt cgtcagtgga attacagtgc gcaaggtgcg gtggttgctc atgctcatgg
     3481 agtaacattg gggcaatcag tgcaggattc attcgcgatc gtgcatatta atgagggtgc
     3541 caatgtaaaa gtacaaaatg cgcaaggcgt ttatactgat tattggggaa acgcgattgt
     3601 tcccaatatg acgaattatc gacataacgc tattactgtg aatacacagg ggcacgatag
     3661 tcttgatatt tctgatgcca cacaagatgt tattccttcg aaaggggctg ttgttggtgt
     3721 cgattttgat gcacgtagcg gaatacgagc cttgttgaca ttagtccata ataaagaacg
     3781 cgtacccttt ggggcattgc tgacattagg gaatagtaca gcgattgttg gtgaagatgg
     3841 ggaggtttat attactggtg tgcaagaatc aatgacattt acggtacagt ggggaaaaga
     3901 gattaaccag caatgtacag gagttgtcac agtgccagaa aaatacacca cgggtatcta
     3961 taaagccaca atggattgcc gataacaaat aagacaggaa aaaataatga agccaggatt
     4021 cgtctcttat atacgaacat tattgaccat aatatttctt agctggggcg tcattcctga
     4081 ggttttcgcc ggttgtaaca tttctggaaa tggaggaaat ccggcaacag tacaattctc
     4141 cgtgcctgcg ttggttgtta atagcgatgc cgttcctgga acgattattg cgacagtaaa
     4201 tgcgaacagt ccgtcaattt cagtttcctg tacggcaaat ggtgagatct ggcaaggata
     4261 taccgtattg acaaacggtg atatgcgtac tgataaccca cttaacaatg tgtttcagac
     4321 taatgtgcca ggaatcggtt ttcgcgcggc gtgggcgaat aacaccagtg cgcaactcac
     4381 cgccggatat ttaatgacgc cctggcataa aggctcgtca acagtgatta aaggatatgg
     4441 ttatccgatt acaatacatg cagctattca gtttattgtc acaggacctg tcggtaatgg
     4501 gaccattgat accagcaaac tggttgctga ctggaagtat gacaatcgtg ttattggaga
     4561 gcttcgtttt agttctgttc cggtcaatat tcaaccgggc acatgcgacc ttatagaaaa
     4621 aaatattacc gttccactca atgatattgg cacagatgat tttgatgtta acaatgtgtc
     4681 acctgtagtt tccgatgatc ggtttaaaat tcagattgaa aaatgttctc aggatgtaaa
     4741 agttgattac cgctttactt catcaggttc tactggagta acgggtaata atattcttaa
     4801 tattgagtct ggttcaggaa tggcggaagg agttggtctg cagatcctcg atagtaataa
     4861 caatgtgatg gtatttgata cagactatac acctgttcaa aaaacgacgt taaatcaaaa
     4921 tgttactatt ccgttaaaag cacgctacat caaaatgggt agcgtcaaaa gtggtcgggt
     4981 taattctgtt gccaccttcg aagtgtatta ccgataagct tttccctttg ccataaatgt
     5041 taatttttgg taatggaatt tttatggttt acggtgcaaa gattaacatt tttgattcat
     5101 ctgattgatt ctcgcccctt catggacgca cagagcgtat atcacttgcg aacccgccag
     5161 cgtttcgaat attatcgcat ctttataata atcattctcg tttacgttat cattcacttt
     5221 catcagagat ataccaatgg cgcgttccaa aactgctcag ccaaaacact cactgcgtaa
     5281 aatcgcagtt gtagtagcca cagcggttag cggcatgtct gtttatgcac aggcagcggt
     5341 tgaaccgaaa gaagacacta tcaccgttac cgctgcacct gcgccgcaag aaagcgcatg
     5401 ggggccagct gcaactattg cggcgcgaca gtccgctacc ggcactaaaa ctgatacgcc
     5461 gattcaaaaa gtacctcaat ctatttctgt cgtcaccgct gaagagatgg cactgcatca
     5521 gcctaagtcg gtgaaagaag cgcttagcta tacccctggc gttgctgtgg gaacccgtgg
     5581 cgcatctaat acttatgatt acctgatcat ccgcggattt gcggcagacg gccaaagcca
     5641 gaacaactat ctgaatggcc tgaagatgca gggcaacttc tataacgatg cggttattga
     5701 cccatatatg ctggagcgcg ctgaaatcat gcgtggcccg gtttccgtgc tgtatgggaa
     5761 aagcagccca ggcggcctgt tgaacatggt cagcaagcgc ccgaccactg aaccgttgaa
     5821 agaagttcag tttaaggccg ggactgacag cctattccag actggttttg acttcagcga
     5881 tgcgctggat gatgacggcg tttactctta tcgcctgacc ggccttgcgc gttctgcgaa
     5941 tgcccagcag aagggggctg aagagcagcg ttatgctatt gctccagcgt ttacctggcg
     6001 tccgaatgat aaaaccaatt tcactttcct ttcttacttc cagaacgagc cggaaaccgg
     6061 ttattacggc tggttgccga aagaggggac tgtcgaaccg ctgccaaacg gtaagcgtct
     6121 gccgaccgac tttaacgaag gcgcgaagaa caacacctat tctcgtaacg agaagatgat
     6181 tggttatagc ttcgaccacg aatttaacga cacctttact gtgcgtcaga acctgcgctt
     6241 tgcacaaaat aaagtctcgc aaaagagcgt atatggctac ggcatgtgct cagacccgct
     6301 ctattcttca aacccctctt cgagtccatg tgctaacgtg ccgcagtctc aatggggcca
     6361 tacactgact cgtcagtacg ttatcgataa tgagaagtta gaaaacttct ctgttgatac
     6421 tcagttgcaa agcaaattcg caaccggttc agtggatcac actctgctga ctggcgttga
     6481 ctttatgcgt atgcgtaatg acatcgattc ctggtttggt tatgccggtt cagttgcacc
     6541 atctgatatc tataatttag accgtagtga ctttgatttt ggcgctcacc cgaatccgtc
     6601 tggtccatac cgcgttctgc tgaaacagaa acaaaccggc ctgtatgttc aggatcaggc
     6661 gcagtgggat aaagtgctgg ttacgcttgg cggtcgctat gactgggccg atcagtcatc
     6721 tttcaaccgt gattacggta ataaatctga acgtgacgac aaagagttca cctggcgtgg
     6781 tggtgtaaac tacctgttcg acaacggcgt aacgccttac ttcagctaca gtgaatcatt
     6841 tgaacccgct tcacagactg atgcgaacgg cgatctgttt gcaccttcga aaggcaaaca
     6901 gtatgaagtc ggcgtgaaat atgtgccaga agatcgccca attgtggtga ctggcgcact
     6961 gtatcagctt accaaaacta ataacctgat ggcggatccg aacggttcac tgttctcggt
     7021 tgaaggcggt gagattcgtg cgcgtggcgt agagctggaa gcgaaagcgg cactgtcggc
     7081 gagtgttaac ttggttggtt cttacaccta tactgacgcg gaatacacca ctgacaccaa
     7141 ctacaaagga aatacgcctg cacaggtgcc aaaacacatg gcttcgctgt gggctgacta
     7201 taccttcttt gacggtccgc tttcaggtct gacgctgggc accggtggtc gttatactgg
     7261 ctccagctat ggtgatccgg ctaactcctt taaagtggga agttatacgg tcgtggatgc
     7321 gttagtgcgt tatgatctgg cgcgagtcgg catggcgggc tccaacgtgg cgctgcatgt
     7381 caacaacctg ttcgatcgtg aatacgtcgc cagctgcttt aacacttatg gttgcttctg
     7441 gggcgcagaa cgtcaggtcg ttgcaaccgc aaccttccgt ttctaatttc tctttttggg
     7501 gcacggattt ccgtgcccgt ttcacaagtt ggctgttatg caggaataca cgaatcattc
     7561 cgataccact tttgcactgc gtaatatctc ctttcgtgtg cccgggcgca cgcttttgca
     7621 tccgctgtcg ttaacctttc ctgccgggaa agtgaccggt ctgattggtc acaacggttc
     7681 tggtaaatcc actctgctca aaatgcttgg tcgtcaccag ccaccgtcgg aaggggagat
     7741 tcttattgat gcccaaccgc tggaaagctg gagcagcaaa gcgtttgccc gcaaagtggc
     7801 ttatttgccg cagcagcttc ctccggcaga agggatgacc gtgcgtgaac tggtggcgat
     7861 tggtcgttac ccgtggcatg gcgcgctggg gcgctttggt gttgcagatc gcgaaaaggt
     7921 cgaggaagct atctcgctgg ttggcttaaa accgctggcg catcggctgg tcgatagcct
     7981 ttctggcggc gaacgtcagc gggcgtggat cgccatgctg gtggcgcagg atagccgctg
     8041 tctgttgctc gacgaaccga cttcggcgct ggatatcgcc caccaggttg atgtactggc
     8101 gctggtgcat cgtttaagtc aggagcgtgg cctgacggtc attgccgtgc tgcacgatat
     8161 caatatggcg gcgcgctact gtgattatct ggtcgcccta cgcggcggtg aaatgattgc
     8221 tcagggaacg cctgcggaaa ttatgcgcgg cgaaaacctc gaaatgattt atggcatccc
     8281 gatgggtatt ctgccgcatc cggcgggtgc tgcacctgtg agttttgttt attgatgagc
     8341 ggcttacctc ttatttcgcg ccgccgactg ttaacggcga tggcgctttc tccgttgtta
     8401 tggcagatga ataccgccca cgcggcggtt attgatccca atcgtattgt ggcactggag
     8461 tggttgccgg tggagttact gctggcgctc ggcatcgtgc cttacggcgt ggcggacacc
     8521 atcaactatc gcctgtgggt cagcgaaccg ccattgccgg actcagtgat tgacgttggt
     8581 ttgcgtacag aacccaacct tgaactgctg accgaaatga aaccgtcgtt tatggtctgg
     8641 tctgcaggat atggcccttc accagaaatg ctggcacgta ttgcgccggg gcgcggattt
     8701 aacttcagcg acggcaaaca gccactggcg atggcgcgta aatcactgac ggaaatggca
     8761 gatttactta acctgcaaag cgcagcggaa acgcatttag cgcactatga agactttatc
     8821 cgcagcatga aaccccgctt tgtgaagcgt ggtgcacgcc cgttattgct gacgacgctt
     8881 atcgatccgc gccatatgtt ggtcttcggt ccaaacagct tgttccagga aattctcgat
     8941 gagtacggca tcccaaatgc ctggcaaggg gaaaccaact tctggggcag taccgccgtc
     9001 agtatcgatc gtctggcagc gtataaagac gttgatgtgc tctgttttga tcacgacaac
     9061 agcaaagaca tggatgcgct aatggcaacc ccgctgtggc aggccatgcc gtttgtccgc
     9121 gccggacgct ttcagcgcgt acctgcagtt tggttctatg gtgcgacgct ttctgcaatg
     9181 cactttgtgc gtattctgga taacgccatc ggaggtaaag cgtgagtaaa cgaattgcgc
     9241 tttttccggt gttattgctg gcgctgctgg tggttgctgc tgcggcgttg acctggatga
     9301 acttctcgca ggcgctgccg cgcagccagt gggcgcaggc cgcctggtcg ccggatattg
     9361 acgtcatcga gcagatgatt tttcactaca gcttgttgcc gcgtctggcg atttcgctgc
     9421 tggtgggcgc gggcctgggg atggtgggcg tgctgtttca gcaagtgctg cgtaacccgc
     9481 tggcggaacc gacgacgttg ggtgttgcaa caggcgcgca actggggatt accgtcacca
     9541 cgctctgggc gatccccggc gcgatggcga gccagtttgc tgcgctggca ggggcttgtg
     9601 ttgttggctt aatcgtcttt ggcgtcgcgt gggggaaacg gctttcgccg gtaacgctga
     9661 tcctcgcggg gctggtagtg agcctttatt gcggcgcaat caatcagtta ctggttatct
     9721 tccatcatga ccaactgcaa agcatgttcc tgtggagcac cggaacgctg acgcaaaccg
     9781 actggggcgg cgttgagcgt ttatggccgc agctgctggg cggcgtgatg ctgacgttat
     9841 tgctacttcg cccgttaact ctgatggggc ttgatgatgg cgtggcgcgc aatctcgggc
     9901 tggccttgtc gctcgctcgt ctggcggcgt tgtcgctggc gattgtcatc agtgcgctgc
     9961 tggtgaacgc ggtggggatt atcggcttta tcggtttgtt cgcgccactg ctggcgaaaa
    10021 tgctgggggc gcggcgtttg ttgccacggt tgatgctggc atcgctgatt ggtgcgttaa
    10081 ttctgtggct ttctgatcaa atcatcctct ggctgactcg cgtgtggatg gaagtgtcta
    10141 ccggttcggt cactgcgttg atcggtgcgc cgctgctact gtggctgctg ccgcgtttac
    10201 gcagcattag cgcgccggat atgaaggtca acgatcgtgt cgcgactgaa cgccaacacg
    10261 tgctggcgtt tgctctcgcg ggcggcgtgc tgctgttgat ggctgtggtg gtggcgctgt
    10321 cgtttggtcg tgatgcgcac ggctggacgt gggcgagcgg ggcgttgctc gaggatttga
    10381 tgccctggcg ctggccgcga attatggcgg cgctgtttgc gggtgtcatg ctggcggtgg
    10441 cgggctgtat tattcagcgg ctgaccggaa acccgatggc aagcccggaa gtgctgggga
    10501 ttagctccgg cgcggcgttt ggcgtggtgt taatgctgtt tctggtgccg ggtaatgcct
    10561 ttggctggct gttacccaca ggcagtctcg gggcggcggt gacgctgttg atcattatga
    10621 tcgccgccgg acgcggtgga ttttccccac accgcatgtt actggcgggg atggcgttaa
    10681 gtaccgcgtt caccatgctt ttgatgatgt tgcaggcaag tggtgacccg cgaatggcgc
    10741 aagttctgac ctggatttcc ggttcgacct acaacgcgac cgatgcgcag gtctggcgta
    10801 ctggaattgt gatggtgatt ttgctggcga ttaccccgct gtgccgccgc tggctgacca
    10861 ttttaccgct gggtggtgat accgcacgag ccgtggggat ggcgctgacg ccgacgcgaa
    10921 ttgcgctgct gctgttagcg gcttgcctga cggcgaccgc gacaatgacc attggtccgt
    10981 tgagttttgt tggtttaatg gcaccgcata ttgcgcggat gatgggcttt cgacggacga
    11041 tgccacacat cgtaatttcg gcgctggtgg gaggattact gctggtgttc gctgactggt
    11101 gtgggcggat ggtattgttt ccattccaga tcccagcggg gctgctgtcg acctttatcg
    11161 gcgcgccata ttttatctat ttgttgagaa agcagagccg ttaataggat gcctcgcccg
    11221 gtgggcgagg caaaatcatc acaacttcgc aaacacccga cgtgcagcat cgatggtgtt
    11281 attgatatct tccatgctgt gcgccacaga cataaagccc gcttcaaacg ctgacggtgc
    11341 cagataaaca ccttcgtcca gcatcatatg gaagaaacgt ttaaagcgtt ccacgtcaca
    11401 ggacatcaca tcctggtagc atgtcacgga ctcggcgtcg gtaaagaaaa taccgaacat
    11461 gccgccaacg tggtttacca ccagcggaat tccggcttct tctgccgctt cccgcagacc
    11521 ttctgccaga cgtgttgtca gctcatccag cgtctcgtga acgcccggct gtgcgacttc
    11581 attcagacag gcgaaaccag cagccatcgc aatcgggtta ccggaaagcg tacccgcctg
    11641 atagaccgga ccggtcgggg ccagcgcatc cattacatca cgacgaccac cgaatgcgcc
    11701 taccggcatt ccaccgccga tgattttgcc gaggcaggtg agatccggtt ccacaccgta
    11761 ataatcctgt gcgccagcta gcgctacgcg gaaaccggtc atcacttcat cgatgatcag
    11821 caacgcgcca aattcgtcgc acagcgcgcg cagacctggc aggaactctg gcagcggtgg
    11881 cacgcagttc atattgcctg ccaccggctc gacgataata caggcaatct cttgcgggta
    11941 ttgctcaaac gcggcgcgta cagaggccag atcgttataa gtacaggtta aggtatgttt
    12001 ggcgaaatct gccggaacac ccggcgaatt tggctggcct aacgtgagtg cgccagaacc
    12061 ggctttcacc agcaagcagt cagcgtgacc gtggtaacaa ccttcaaatt taataatttt
    12121 gtcgcgaccg gtaaaaccac gggccaggcg gatggcgctc atcgtcgcct cggtgccgga
    12181 gttcaccatg cgcaccatat ccatggtcgg gaccagttcg gtcaccagtt gcgccatttt
    12241 cacttccatt tcggttggtg caccaaagct taaaccacgc tcggcggctt caatcacggc
    12301 attgcggatc gccggatggt tatggcccag caccatcggc ccccaggaac cgacataatc
    12361 gatataggct ttgccatcaa catcgtacag ataagcgccg tccgcttttt cgataaacag
    12421 tggagtgccg cccacgccag taaaggcgcg aacaggggag ttcacgccgc cagggatcag
    12481 ctcgcgcgct gcgctgtaaa gattttcaga cttactcatg gagggttcct gattcgtaga
    12541 aaaagtgaat ggctgctatt ctatgttatt cataacaagt taaatactcg tcaaacatca
    12601 ggctgcttgt actggtgcaa tcctgatttc ttagagtata aaagttttgt gcatttgaaa
    12661 cattacgctt tgcaaaggat tttcatggaa cgtgcgagta aaatgccgtc atcttatttg
    12721 tatgaccaat aagtgatcat tggatgaaaa ctgatactcc ctctttagaa acaccgcagg
    12781 ccgcgcgcct gcgacgcaga caactgattc gccaacttct tgagcgcgat aaaaccccgt
    12841 tagccatttt gtttatggcg gcagtcgtcg gcacgcttgt cgggctggct gcggttgctt
    12901 ttgacaaagg tgtcgcctgg ttgcagaacc aacgtatggg ggcactggta catactgctg
    12961 ataattatcc gcttctgtta accgtcgctt ttctctgttc ggcggtgctg gcgatgttcg
    13021 gctacttttt ggtgcgcaaa tacgcgccgg aagcaggtgg ttcggggatc ccggaaattg
    13081 aaggggcgct ggaagatcaa cgtcctgttc gctggtggcg tgtgttgccg gtgaaattct
    13141 ttggtgggct ggggacactc ggcggaggca tggtgttggg gcgcgaaggg ccaaccgtgc
    13201 agataggcgg caatatcggg cggatggtac tcgatgtttt ccgcctgaaa ggtgacgaag
    13261 ctcgtcatac gctactggca accggtgctg ctgcggggct ggctgcggcc tttaacgcgc
    13321 cgctggcggg tattttgttt attatcgaag agatgcgtcc gcagtttcgc tatacgttaa
    13381 tttcgattaa agcggtattt attggcgtca ttatgtcgac cattatgtac cggattttta
    13441 atcatgaagt tgcgttgatt gacgtcggta aactttctga tgcgccgctt aatacgctgt
    13501 ggctttatct gatcctcggt attatttttg gcattttcgg ccctattttt aataaatggg
    13561 tgctggggat gcaggatttg ctgcaccgtg tgcacggtgg caatattacc aaatgggtac
    13621 tgatgggcgg tgcgattggc ggtttgtgtg gattgctggg gtttgtggca ccagcaactt
    13681 cgggcggcgg ttttaacctg attcctatcg ctaccgcggg gaatttcagc atgggaatgc
    13741 tggtgtttat cttcgtcgcg cgggtcatta ccaccttact ctgcttctct tccggcgcgc
    13801 cgggcggtat ttttgccccg atgctggcgc tgggtactgt gctgggaacc gctttcggaa
    13861 tggttgccgt tgagttgttt ccgcaatatc accttgaggc ggggacgttt gctattgccg
    13921 gaatgggggc gttactggcg gcatctattc gcgcgccgtt aacggggatc attctggttc
    13981 tggaaatgac cgataactac cagctcattt tgccaatgat tattaccggt cttggcgcaa
    14041 cactattagc acaatttacc ggtgggaaac cgctatactc ggcgattctt gcgcgcacgc
    14101 tggcaaaaca ggaagctgag caactggcgc gaagcaaggc cgcatcagcc agcgagaata
    14161 cttgaacgaa ataccagggt attagataat ggcgattatt attgggttag aatttgccca
    14221 attgccgatg tcgtttggag caaaatatga gtgatgacgt agcactgccg ctggagttta
    14281 ccgacgcagc agccaacaaa gttaaaagcc tgatcgctga cgaagataac ccgaatctga
    14341 aattacgcgt gtatatcacc ggtggcggtt gcagcggctt ccagtatggt ttcacctttg
    14401 atgatcaggt gaacgaaggc gatatgacca tcgaaaaaca gggcgttggc ctggtggttg
    14461 atccgatgag cctgcaatat ctggtcggcg gttccgttga ctataccgaa ggtctggaag
    14521 gttctcgttt catcgtgacc aacccgaacg cgaaaagcac ctgcggttgc ggttcttcct
    14581 ttagtatcta atctgctgtt catgtgccgg ataaactatc cggcacatct cctctcttca
    14641 acgaccattc tcatccagcg caaacgtcgg tagcttaaga tgccaacgaa tagccgccag
    14701 ccgaatcaat agcgtcacga ccatgcccat catactggct gtttccagtg gtacggaaaa
    14761 tgtgtaataa gccgtagcgt ggacaatacc gccgataata caggcagttg cgtagatttc
    14821 tgtacgtaaa atcatgggga tttcgcgggc cagtacatca cgaatgattc cgccgccaac
    14881 gccagtaatg acgcccatac aaaccgcgat taacggaccg gcttccgcat taaaggcttt
    14941 attcacgcca atgccgacaa acaccgccag accaacggcg tccagcaccg gcaacatcca
    15001 ttttggtaag cgtcttggct ggcgcaccag cacgatggtc agcatgctgg tgaccattgc
    15061 aacgaccaga tcggtgggat ctttcaccca aaataccggg ccgtgatcca gcgccatgtc
    15121 gcgaattgtt ccgccgccta ctgcggtaac cacgcccaat accagaacac caaaagggtc
    15181 catacgcaat tttccggcta acaaaacgcc agagatagca aataccgctg tgccgactat
    15241 atccagccaa tagacgagca ttgttcaatc cccactgagc acctgaaaag gtcaggcact
    15301 aatctacctg tgaaagcgca ttacagagct gttgtgcagc gaggataata cgtgggcttg
    15361 cacgttcaaa ccagtcactc gtgagaggaa taacgggaat tttgagctgt tcaccccagt
    15421 attgtttgat tttaggaatt tggtccggtc cgcctgtaat gacaatcgcc tgtggcgagc
    15481 gtgctaacac ctgttcgcgg ctaacttgcg gccagggaac ccggctgtct ttaaagatgt
    15541 tttctccgcc acaaacttcg agtacctggt tctgaatcga ctcttttcca ctggtaaatg
    15601 gcggattaat gccgaattgc agaaaaacac gttttttagg tttatcagca tattgcgctt
    15661 tcaattgcgc gtactgatcc agcagggatt gcgcggcttg ttcggcctta tccggttgcg
    15721 gactccaggg ggccagttga cgtaacgcat tggcaatttg ttcaatgctt gtcgcatcga
    15781 cccacatcac ttttattccc agcgaagcca gctggtcaac ctgccgctcg gcattacctc
    15841 cacgccaggc aatcaccaga tcgggtttca gcgcgacaat gcgttccaga ttcatcccct
    15901 gccaggtgga aacctgctca atcttttgcg cttgtggagg atagtcggaa tagctgctga
    15961 ccccaaccgg cgtgatcccg gcggcaaagg caagttcagt gttggcggga gaaagcgtga
    16021 tgacgcgcgg cgcggcattg agccacagcg gcgcgagaaa agacagggcg accagcgccc
    16081 tgaacagtga cttagccatg tgccagtttc tgcaccagtg actcaaccat caggctggac
    16141 tgtttagcgg caacagccag gaactcatcg aagctaagat gagactgttg atcggccacg
    16201 tcggagatgg cgcgtactac gacaaacggg acgttgaaat tgtggcagac atgggcgatt
    16261 gccgtcgctt ccatctctac agcaatggcc tgtgggaagt tgtggcggat tttcgccaga
    16321 ccaacagaac cgttgatgaa agcgtcgccg ctaacaatca ggccacgtac agcgttaaga
    16381 ttcagttcgg caatgcaggc ctcagcggca gcgatcagtt tatcgtcagc tttgaagcct
    16441 gccgggcagc ctggtaactg accgtattca taaccaaatg ccgtgacatc cgcgtcgtga
    16501 taacgcgctt cgtccgagac aacgatatcg cccactttca gcgttggtgc caggccaccg
    16561 gcagaaccgg tgttaataat cacatctggc ttgcagtgtt ccaacagcaa agtggcaccc
    16621 agcgccgcag cgactttacc gatgcccgat ttcagaagcg caacctcggt tccattcagt
    16681 tggccggtat agatttcgca accgcccaga ctgatagttt gacggttttc gattttgtca
    16741 cgcagcagcg taacttcttc ttccattgca ccaatgatgc cgattttcat agatttactc
    16801 gcgataagcc cgatttgaag gcatagttta ccatgcgctt acggggaagc gtatttctca
    16861 cgcgggagag gacatggcac agattgattt ccgaaaaaaa ataaactggc atcgtcgtta
    16921 ccgttcaccg cagggcgtta aaaccgaaca tgagattctg cggatcttcg agagcgatcg
    16981 cgggcgtatc atcaactctc cggcaattcg tcgtctgcaa caaaagaccc aggtttttcc
    17041 actggagcgc aatgccgccg tgcgcacgcg tcttacccac tcgatggaag tccagcaggt
    17101 ggggcgttat atcgctaaag agattttaag ccgcctgaaa gagcttaaat tactggagac
    17161 gtatggtctc gatgaactga ccgggccctt tgaaagcatt gttgagatgt catgcctgat
    17221 gcacgatatc ggcaatccgc cgtttggtca ttttggcgaa gcggcgataa atgactggtt
    17281 tcgccagcgt ttgtacccgg aagatgccga aagccagcct ctgactgacg atcgctgcag
    17341 cgtggcggca ctacgtttac gggacgggga agaaccgctt aacgcgctgc ggcgcaagat
    17401 tcgtcaggac ttatgtcatt ttgaaggtaa cgcacagggt attcgtttgg tgcatacatt
    17461 gatgcggatg aatctcacct gggcgcaggt tggcggtatt ttaaaatata cccgtccggc
    17521 gtggtggcgt ggtgaaacgc ctgagacaca tcactattta atgaaaaagc cgggctatta
    17581 tctttccgaa gaagcctata ttgcccggtt gcgtaaagaa cttaatttgg cgctttacag
    17641 tcgttttccg ttaacgtgga ttatggaagc tgccgacgac atctcctatt gtgtggcaga
    17701 ccttgaagat gcggtagaga aaaggatatt taccgttgag cagctttatc atcatttgca
    17761 cgaggcgtgg ggccagcatg agaaaggttc gctcttttcg ctggtggttg aaaatgcctg
    17821 ggaaaaatca cgctcaaata gtttaagccg cagtacggaa gatcagtttt ttatgtattt
    17881 acgggtaaac accctaaata aactggtacc ctacgcggca caacgattta ttgataatct
    17941 gcctgcgatt ttcgccggaa cgtttaatca tgcattattg gaagatgcca gcgaatgcag
    18001 cgatcttctt aagctatata aaaatgtcgc tgtaaaacat gtgtttagcc atccagatgt
    18061 cgagcagctt gaattgcagg gctatcgggt cattagcgga ttattagaga tttatcgtcc
    18121 tttattaaac ctgccgttat cagactttac tgaactggta gaaaaagaac gggtgaaacg
    18181 tttccctatt gaaacgcgct tattccacaa actctcgacg cgccatcggc tggcctatgt
    18241 cgaggctgtc agtaaattac cgtcagattc tcctgagttt ccgctatggg aatattatta
    18301 ccgttgccgc ctgctgcagg attatatcag cggtatgacc gacctctatg cgtgggatga
    18361 ataccgacgt ctgatggccg tagaacaata accaggcttt tgtaaagacg aacaataaat
    18421 ttttaccttt tgcagaaact ttagttcgga acttcgcgct ataaaacgaa tctgaagaac
    18481 acagcaattt tgcgttatct gttaatcgag actgaaatac atgaaaaaaa ccacattagc
    18541 actgagtgca ctggctctga gtttaggttt ggcgttatct ccgctctctg caacggcggc
    18601 tgagacttct tcagcaacga cagcccagca gatgccaagc cttgcaccga tgctcgaaaa
    18661 ggtgatgcct tcagtggtca gcattaacgt agaaggtagc acaaccgtta atacgccgcg
    18721 tatgccgcgt aatttccagc agttcttcgg tgatgattct ccgttctgcc aggagggttc
    18781 tccgttccag agctctccgt tctgccaggg gggcccgggc ggtaatggcg gcggccagca
    18841 acagaaattc atggcgctgg gttccggcgt catcattgat gccgataaag gctatgtcgt
    18901 caccaacaac cacgttgttg ataacgcgac ggtgattaaa gtccagctta gcgatggtcg
    18961 taagttcgac gcgaagatgg ttggcaaaga tccgcgctct gatatcgcgt tgatccagat
    19021 ccagaacccg aaaaacctga ccgcaattaa gatggcggat tctgatgcgc tgcgcgtggg
    19081 tgattacacc gtagcaattg gtaacccgtt cggcctgggc gagacagtaa cttccgggat
    19141 tgtctctgcg ctgggacgta gcggcctgaa cgctgaaaac tacgaaaact tcatccagac
    19201 cgatgcagcg atcaaccggg gtaactcagg tggtgcgttg gttaacctga acggcgaact
    19261 gattggtatc aacaccgcga tcctcgcacc ggacggcgga aacatcggta tcggttttgc
    19321 tatcccgagc aacatggtga aaaacctgac ctcgcagatg gtggaatacg gccaggtgaa
    19381 acgtggtgag ttgggtatta tgggcactga gctgaactcc gatctggcga aagcgatgaa
    19441 agttgacgct cagcgcggtg ctttcgtaag ccaggttctg ccgaattctt ccgccgcgaa
    19501 agcgggcatt aaagcgggtg atgtgatcac ctcactgaac ggtaagccaa tcagcagctt
    19561 tgccgcactg cgtgctcagg tgggcactat gccggtaggt agcaaactga ccctgggctt
    19621 actgcgcgac gggaagcagg ttaacgtgaa cctggaactt cagcagagca gccagaatca
    19681 ggttgattcc agcaccatct tcaacggcat tgaaggcgct gagatgagta acaaaggcaa
    19741 agatcagggc gtggtggtga acaacgtgaa aacggggact ccggctgcgc agatcggcct
    19801 gaagaaaggt gatgtgatta ttggcgcgaa ccagcaggca gtgaaaaaca tcgctgaact
    19861 gcgtaaagtt ctcgacagca aaccgtctgt gctggcactg aacattcagc gcggcgacag
    19921 cactatctac ctgttaatgc agtaatctcc ctcaacccct tcctgaaaac gggaaggggt
    19981 tctccttaca atctgtgaac ttcaccacaa ctccatacat cttcatcatc ctttgggcat
    20041 ttgcacaatg ccgtacgtta cgtacttcct tatgctaagc cgtgcataat ggaggactta
    20101 tggctggttg gcatcttgat accaaaatgg cgcaggatat tgtggcacgt accatgcgca
    20161 tcatcgatac caatatcaac gtaatggatg cccgcgggcg aattatcggc agcggcgatc
    20221 gtgagcgtat tggtgaattg cacgaaggag cattgctggt actttcacaa ggacgagtcg
    20281 tcgatatcga tgacgcggtg gcacgtcatc tgcacggtgt gcggcagggg attaatctac
    20341 cgttacggct ggaaggtgaa attgtcggcg taattggcct gacaggtgaa ccagagaatc
    20401 tgcgtaaata tggcgaactg gtctgcatga cggctgaaat gatgctggaa cagtcgcggt
    20461 tgatgcacct gttggcgcag gatagccgtt tgcgggaaga actggtgatg aacctgattc
    20521 aggctgagga gaatactccc gcacttactg aatgggcgca acggctgggg atcgatctca
    20581 atcaaccgcg agtggtggct attgttgagg tcgacagcgg tcagcttggt gtggacagcg
    20641 caatggcgga gttacagcaa ctgcaaaatg cgctgactac gcccgagcgt aataatctag
    20701 tggcgattgt ctcgctaacc gaaatggtgg tgttgaaacc ggcgttgaac tcttttgggc
    20761 gctgggacgc agaagatcat cgtaagcgag ttgaacaact gattacccgc atgaaagagt
    20821 atggccagct gcgttttcgc gtttcgctgg gcaactattt taccggtcct ggcagtattg
    20881 cccgatccta tcgtacggcg aaaacgacga tggtggtggg taaacagcgg atgccagaaa
    20941 gtcgctgcta tttttatcag gatctgatgt tacctgtgtt actcgacagt ttgcgtggcg
    21001 actggcaggc caacgaactg gcgcgaccgc tggcgcggct gaaagcgatg gacaataacg
    21061 gcttgctgcg gcgaactctg gcggcgtggt ttcgccacaa tgtgcaaccg ctggcaacgt
    21121 caaaggcgtt gtttattcat cgtaatactc tggaatatcg gcttaatcgt atatcggaac
    21181 tgaccgggct tgatttgggc aattttgatg acaggttgct gctgtatgtg gcgttacaac
    21241 tggatgaaga gcggtaggtc gattacaaaa ttacagccgg gtgagacttt acccggccca
    21301 cagaatgaca ttatttacgc gtcagttttt ccagatcggc ttcaatctcg ctgatcttat
    21361 tggttactac tgactccagg tgacgtaagt catcgaggat cttacgctta agatcaactt
    21421 cgctgcggtc acgctggcag atttgatcaa gctcatcaat gatataccgt agattcgggc
    21481 tgatttcctg gacttcttta taaccctgcc ccacaccatc agcgacaacc gttttacgct
    21541 gacgcggata tttaaacttc acgctcttgg cgaaaaactc gcctttgtct ttctggaaat
    21601 agattttcag aatatcgttg ttggcttcct gccggaggct gtaacgatca atttcttcag
    21661 gattggtaat acccagactt ttcagattgt cgtacatagc gttacctcaa aatgagtcag
    21721 taaattgtgc ttattttagc atttggcctg gcccgcccgg ctcgactgtg atcgcagaaa
    21781 gcctttttct tccagacttc tatcatggcg taaaaatcaa aaaattacct gctttattgt
    21841 gatgatattc catgggatgg attagcacat ttatcttcat tttgcgaatt acatgacagt
    21901 ccgaaacaag atgaaaacgt atttaaaaaa gggggaaaat gagtaggacg aattattctg
    21961 agccagaaag caatcgggca gcgtcgtgct gcccgtgtac atacttttag tcgatggtac
    22021 gcagcagttc gttaatacca actttaccgc gagttttcgc gtcaactttc ttaacgataa
    22081 ctgcacagta gagactgtat ttgccatctt tcgacggcag attacctgaa acaaccacag
    22141 accctgccgg aacgcgaccg tagtggattt cgccggtttc acggtcataa atacgggtgc
    22201 tctgaccaat gtatacgccc atagagatga cggaaccttc ttcgacaatc accccttcaa
    22261 ccacttcaga gcgtgcgccg atgaagcagt tatcttcaat aatggtcggg ttagcctgca
    22321 gcggctccag cacgccgccg atacccacgc caccggaaag gtggacgttt ttaccaatct
    22381 gcgcacagga accgacggtc gcccaggtat caaccatggt gccttcatca acatatgcgc
    22441 cgatgttgac gtaagacggc atcagcacgg tgttacgggc aatgaacgcg ccctgacgca
    22501 ctgccgctgg tggcacgacg cggaagcctt ctttctggaa acgtgcttca tcgtagttag
    22561 cgaatttcat cggcactttg tcgaagtagc ggctttctgc cccttcgatc acctgattat
    22621 cattaatacg gaaagagagc agcaccgctt ttttcaacca ctgatgcgtc acccactgac
    22681 cgtcaatttt ttccgctaca cgcagtgcgc cggaatccag cagggcgatc acctgattta
    22741 ccgcttcgcg ggtaacggtg tctgcattgg ctggcgtgat ctcggcacgg cgttcaaaag
    22801 cggtttcaat aatgttctgt aactgctgca ttgttaaact cttttcatat cagtaaacac
    22861 atcacccttt atcgtttgga ttgagggcct ctgtcaaccg ctgatgcact tcctgctgca
    22921 actcgttatt aagcgcacgc cggtcagcgg tggcaattat gaataaatct tctactcgct
    22981 cgccaatggt tgtaattcgg gcgccatgaa gcgaaattcc cagatcggca aaaattttcc
    23041 cgactcgcgc cagcagtcca ggttggtcga gggcgatcag ttcgaggaac gatttgcggt
    23101 cggtatgggt cggcaaaaac gtcacttcgg tttcaacagt aaaatggcgc aatttagcgg
    23161 gttggcgacg gggctgcggt ggctgccagc tactttgtgt cagcacctgc tccagaccaa
    23221 accgaataac ctcatgacga tctgcggata gcgggctacc atcgggttcc agcactataa
    23281 aggtatccat cgccataccg tcgcgagtgg tgaaaatttg tgcgtcgtga acacttaaat
    23341 tgcggcggtc taattcggca cagacggcgg caaacagata ggggcggtcc gggctccaga
    23401 taaaaatctc ggtgcctcca cgcgtagcct gcgggctaag cagtaccagc ggtttgctta
    23461 aatcatgctg taataaatgg cgtgcgtgcc aggccagttg atttgggcta tggcggacaa
    23521 aatagttagc gcgacagcgt gaccaaattt ggtgcagcgc ctcttcgtcg atgttatcca
    23581 tgcgcagtag tgccagcgcc tggagttggt gatggcgaac ccgttcgcgc atatccggcg
    23641 tgttttgcat tccgcgtcgt agctgctttt cggtggcaaa atagagttcg cgcaacagac
    23701 tttgcttcca gctattccac agtgtttcgt tggtggcgca aatgtcagcc actgtcaggc
    23761 ataccagata gcgcagacga ttttccgttt ggacttcttc ggcaaactgc ttgatgactt
    23821 ccgggtcctg aatatcgcgg cgctgagctg tcaccgacat cagcaagtgc tggcgaacca
    23881 gccaggcgac cagctgtgtt tcgcgtgagt tcagcccgtg gagttcggca aaatgcacta
    23941 catcctgagc accgagaatg gagtggtcgc cgccgcgtcc tttggcgata tcgtgaaaca
    24001 gcgcggcgat gaaaatcagc tcagttgacg gcagacgcgg ccagacgtcc acacacaacg
    24061 gatggcgctg gcgcgtttct tcactggcaa aactctccag tttcaacatc acgcggatag
    24121 tatgttcatc caccgtgtag gcgtggaaca gatcaaactg catctgcccg acgatatgcg
    24181 accattgcgg catatacgcg ccgagtacgc tatggcgatg cattggcaat agcccgcgcc
    24241 gcaccgctcc ggggtgacgc agaatgctca aaaacagttt tcgcgcttcc ggaatattac
    24301 acagaggttg ttgcagatgg cgacgggcgt ggcgtaactg gcgtagcgtg gtggagtaaa
    24361 tgccggtgat cgcactgttg cgcaccatgg tgtagaacat acgcaagatg gcttccggct
    24421 ggcgcataaa tagtgtttca tcacgcaggt cgatcagcgt tccgcgtagc tgaaactcat
    24481 cgtcgattgg acgtggtttt tcgtcggcgg gaagggcgag gatggcttca tcgaacagtt
    24541 gcagcagcat ctggttgagt tcactgacgc ggcgtgtaac gcggaagtaa tccttcatca
    24601 tccgctcgac cggttcgtta ccttcaccac tgtaattcaa acgctgggcg acgctaagct
    24661 ggcgatcgaa taacaggcga ttatcgtaac gactgacgac cagatgcagg gcaaagcgaa
    24721 tgcgccacaa tatatgcagg cattcgttta attccgctcg ctccgcagag gttaagaagc
    24781 caaaccctac catttcatcc agcgatgttg cgccaaaatg acggcgggcc acccattgca
    24841 gagtgtggat atcgcgcaag ccgccggggc tacttttgat gtctggttca aggttgtagc
    24901 tggtgccgtg gtaacgctga tggcgctggt tctgttcttc aactttcgcc gcgtagaact
    24961 tgtcggaagg ccagaatcct tcgctaaaaa tatgtttttg cagttcgagg aacagcgcaa
    25021 catcgccaat taataagcgg gattcgatta aattggtggc gacggttaaa tccgataacc
    25081 cttccagcat gcactcttca agcgtgcgca cgctatgacc cacttccagc tttacatccc
    25141 agagcagcgt taacagctcg cccacttttt gcgcctgatc gtccgggagc tttttacggc
    25201 ttaaaatcag taaatcgatg tcagaaagtg gatgcagctc gccacgaccg tagccaccga
    25261 cggcgaccaa tgccaggtcg gcaatctggc tgaatcccgc ttcaatccat aatcgttgaa
    25321 gaagctggtc gataaactcg gtacgcgcct caatcaactg ttctgcggag ataccattgt
    25381 caaaggcatc acccagccaa cgctggaaag tatcgatatg ggcttttatc ccaccgacgg
    25441 ttaattcatc acggggccag gcgcatggat tttgcggttg accgtgcagg gtggggagag
    25501 cggtgtttgc gtactgttct ggaagggtat tcattgtgcg ccacccataa gattaaatta
    25561 ttgcattaaa aaagccggca ttcgccggct tcattttatt cgtcgtgcga gattatcgcc
    25621 gggatggtgt catccttgcg tagcgtcaga atttcgcagc cgttatcagt caccacaata
    25681 gtatgctcat attgtgcaga caagctgcga tctttggttt ttaccgtcca gccatctttc
    25741 atggtgcgga tctctttttt acccgcgttg accattggct cgatggtgaa tgtcatcccc
    25801 ggtttcagca cgacgttggt ttcacgggag tcatagtgca gcacctgcgg ttcttcgtgg
    25861 aagccgcgac caataccgtg tccgcaatat tcacgaacga cggagaagcc ttctgcttcg
    25921 acaaatttct gaattgccgc accgatttcg cgcagattaa tgcctggttt taccatgcgt
    25981 agcgccaggt acaggctttc ttgcgtgatg cggcacagac gttcgcccat gatggtcggc
    26041 ttaccgacga taaacatttt cgaggtatcg ccgtggaaac catctttgat tacggtgaca
    26101 tcaatgttaa cgatatcgcc atctttcagc agcttggcgt cgtccgggat accgtggcac
    26161 accacttcat taatagagat gcaaacggat ttcggatagc cgtgatagcc gaggcaggca
    26221 gaaaccgcgt gttgttcatt aacaatgtaa tcattacaga tgcgatccag ctcgccggtg
    26281 ctgacgcccg gtttaacata cggttcgatc atctccagca cttcggcagc cagtcggcca
    26341 gcgacgcgca ttttttcgat atcttctggg gtcttgattg agatagccat taattctgtc
    26401 catcagcgtc ggtgataccg acaatatata tgtaagtgcc gtcaatggta tcacacccgg
    26461 gcaaattgag aatcattctg aatttcgcca aacgtgccac tgaaggtttt ctataataga
    26521 aaattcaacg tctgatgctg tacacagcgc caacaattat tggtgtccac gacgtatttg
    26581 tggtataaag cgcgccggac ttccgatcca tttcgtatac acagactgga cggaagcgac
    26641 aatctcactt tgtgtaacaa cacacacgta tcggcacata ttccggggtg ccctttgggg
    26701 tcggtaatat gggatacgtg gaggcataac cccaactttt atatagaggt tttaatcatg
    26761 gcaactgttt ccatgcgcga catgctcaag gctggtgttc acttcggtca ccagacccgt
    26821 tactggaacc cgaaaatgaa gccgttcatc ttcggtgcgc gtaacaaagt tcacatcatc
    26881 aaccttgaga aaactgtacc gatgttcaac gaagctctgg ctgaactgaa caagattgct
    26941 tctcgcaaag gtaaaatcct tttcgttggt actaaacgcg ctgcaagcga agcggtgaaa
    27001 gacgctgctc tgagctgcga ccagttcttc gtgaaccatc gctggctggg cggtatgctg
    27061 actaactgga aaaccgttcg tcagtccatc aaacgtctga aagacctgga aactcagtct
    27121 caggacggta ctttcgacaa gctgaccaag aaagaagcgc tgatgcgcac tcgtgagctg
    27181 gagaaactgg aaaacagcct gggcggtatc aaagacatgg gcggtctgcc ggacgctctg
    27241 tttgtaatcg atgctgacca cgaacacatt gctatcaaag aagcaaacaa cctgggtatt
    27301 ccagtatttg ctatcgttga taccaactct gatccggacg gtgttgactt cgttatcccg
    27361 ggtaacgacg acgcaatccg tgctgtgacc ctgtacctgg gcgctgttgc tgcaaccgta
    27421 cgtgaaggcc gttctcagga tctggcttcc caggcggaag aaagcttcgt agaagctgag
    27481 taataaggca cgctcgtacg agcccttata gaccaggtag tacacgtttg gttagggggc
    27541 ctgcatatgg cccccttttt cacttttata tctgtgcggt ttaatgccgg gcagatcaca
    27601 tctccgagga ttttagaatg gctgaaatta ccgcatccct ggtaaaagag ctgcgtgagc
    27661 gtactggcgc aggcatgatg gattgcaaaa aagcactgac tgaagctaac ggcgacatcg
    27721 agctggcaat cgaaaacatg cgtaagtccg gtgctattaa agcagcgaaa aaagcaggca
    27781 acgttgctgc tgacggcgtg atcaaaacca aaatcgacgg caactacggc atcattctgg
    27841 aagttaactg ccagactgac ttcgttgcaa aagacgctgg tttccaggcg ttcgcagaca
    27901 aagttctgga cgcagctgtt gctggcaaaa tcactgacgt tgaagttctg aaagcacagt
    27961 tcgaagaaga acgtgttgcg ctggtagcga aaattggtga aaacatcaac attcgccgcg
    28021 ttgctgcgct ggaaggcgac gttctgggtt cttatcagca cggtgcgcgt atcggtgttc
    28081 tggttgctgc taaaggcgct gacgaagagc tggttaaaca catcgctatg cacgttgctg
    28141 caagcaagcc agaattcatc aaaccagaag acgtatccgc tgaagtggta gaaaaagaat
    28201 accaggtaca gctggatatc gcgatgcagt ctggtaagcc gaaagaaatt gcagagaaaa
    28261 tggttgaagg ccgcatgaag aaattcaccg gcgaagtttc tctgaccggt cagccgttcg
    28321 ttatggaacc aagcaaaact gttggtcagc tgctgaaaga gcataacgct gaagtgactg
    28381 gctttatccg cttcgaagtg ggtgaaggca tcgagaaagt tgagactgac tttgcagcag
    28441 aagttgctgc gatgtccaag cagtcttaat tatcgaaaag gagccgcctg agggcggctt
    28501 ctttttgtgc ccatcttgta aattcagcta acccttgtgg ggctgcgctg aaaagcgacg
    28561 tacaatgtcg ctagtattaa ttcatttcaa tcgttgacag tctcaggaaa gaaacatggc
    28621 taccaatgca aaacccgtct ataaacgcat tctgcttaag ttgagtggcg aagctctgca
    28681 gggcactgaa ggcttcggta ttgatgcaag catactggat cgcatggctc aggaaatcaa
    28741 agaactggtt gaactgggta ttcaggttgg tgtggtgatt ggtgggggta acctgttccg
    28801 tggcgctggt ctggcgaaag cgggtatgaa ccgcgttgtg ggcgaccaca tggggatgct
    28861 ggcgaccgta atgaacggcc tggcaatgcg tgatgcactg caccgcgcct atgtgaacgc
    28921 tcgtctgatg tccgctattc cattgaatgg cgtgtgcgac agctacagct gggcagaagc
    28981 tatcagcctg ttgcgcaaca accgtgtggt gatcctctcc gccggtacag gtaacccgtt
    29041 ctttaccacc gactcagcag cttgcctgcg tggtatcgaa attgaagccg atgtggtgct
    29101 gaaagcaacc aaagttgacg gcgtgtttac tgctgatccg gcgaaagatc caaccgcaac
    29161 catgtacgag caactgactt acagcgaagt gctggaaaaa gagctgaaag tcatggacct
    29221 ggcggccttc acgcttgctc gtgaccataa attaccgatt cgtgttttca acatgaacaa
    29281 accgggtgcg ctgcgccgtg tggtaatggg tgaaaaagaa gggactttaa tcacggaata
    29341 attcccgtga tggataaata agggtaagat tccgcgtaag tatcgcgggg gatcttaccc
    29401 gtaatatgtt taatcagggc tatacttagc acacttccac tgtgtgtgac tgtctggtct
    29461 gactgagaca agttttcaag gattcgtaac gtgattagcg atatcagaaa agatgctgaa
    29521 gtacgcatgg ataaatgcgt agaagcgttc aaaacccaaa tcagcaaaat acgcacgggt
    29581 cgtgcttctc ccagcctgct ggatggcatt gtcgtggaat attacggcac gccgacgccg
    29641 ctgcgtcagc tggcaagcgt aacggtagaa gattcccgta ctctgaaaat caacgtgttt
    29701 gatcgttcaa tgtctccggc cgttgaaaaa gcgattatgg cgtccgatct tggcctgaac
    29761 ccgaactctg cgggtagcga catccgtgtt ccgctgccgc cgctgacgga agaacgtcgt
    29821 aaagatctga ccaaaatcgt tcgtggtgaa gcagaacaag cgcgtgttgc agtacgtaac
    29881 gtgcgtcgtg acgcgaacga caaagtgaaa gcactgttga aagataaaga gatcagcgaa
    29941 gacgacgatc gccgttctca ggacgatgta cagaaactga ctgatgctgc aatcaagaaa
    30001 attgaagcgg cgctggcaga caaagaagca gaactgatgc agttctgatt tcttgaacga
    30061 caaaaacgcc gctcagtaga tccttgcgga tcggctggcg gcgttttgct ttttattctg
    30121 tctcaactct ggatgtttca tgaagcaact caccattctg ggctcgaccg gctcgattgg
    30181 ttgcagcacg ctggacgtgg tgcgccataa tcccgaacat ttccgcgtag ttgcgctggt
    30241 ggccggcaaa aatgtcactc gcatggtaga acagtgcctg gaattctctc cccgctatgc
    30301 cgtaatggac gatgaagcga gtgcgaaact tcttaaaacg atgctacagc aacaggggag
    30361 ccgcaccgaa gtcttaagtg ggcaacaagc cgcttgcgat atggcagcgc ttaaggatgt
    30421 tgatcaggtg atggcagcca ttgttggcgc tgctggactg ttacctacgc ttgctgcgat
    30481 ccgcgcgggt aaaaccattt tgctggccaa taaagaatca ctggttacct gcggacgtct
    30541 gtttatggac gccgtaaagc agagcaaagc gcaattgtta ccggtcgata gcgaacataa
    30601 cgccattttt cagagtttac cgcaacctat ccagcataat ctgggatacg ctgaccttga
    30661 gcaaaatggc gtggtgtcca ttttacttac cgggtctggt ggccctttcc gtgagacgcc
    30721 attgcgcgat ttggcaacaa tgacgcccga tcaagcctgc cgtcatccga actggtcgat
    30781 gggacgtaaa atttccgtcg attcggctac catgatgaac aaaggtctgg aatacattga
    30841 agcgcgttgg ctgtttaacg ccagcgccag ccagatggaa gtgctgattc atccgcagtc
    30901 agtgattcac tcaatggtgc gctaccagga cggcagtgtt ctggcgcagc tgggtgaacc
    30961 ggatatgcgt acgccaattg ctcacaccat ggcatggccg aatcgcgtga actctggcgt
    31021 gaagccgctt gatttttgca aactaagtgc gttgacattt gccgcaccgg attatgatcg
    31081 ttatccatgt ctgaaactgg cgatggaggc gtttgaacaa ggccaggcag cgacgacagc
    31141 attgaatgcc gcaaatgaaa tcaccgtggc tgcttttctt gcgcaacaaa tccgctttac
    31201 ggatatcgct gcgttgaatt tatccgtact ggaaaaaatg gatatgcgcg aaccacaatg
    31261 tgtggatgat gtgttatctg ttgatgcgaa cgcgcgtgaa gtcgccagaa aagaggtgat
    31321 gcgtctcgca agctgaggat aatccggcta cagagagtcg cgctatttgt tagcgtaggg
    31381 cttcagtgat atagtctgcg ccatctgatc gtaagtagtt ggctttataa ggtcaggtat
    31441 gccgtggttt tacacggctt ttttttgtat aggcttcagt attcctgagt accgtaaacc
    31501 ctgtcaggga ataagaaacg cgtgatgttg tctgctactc aaccacttag cgaaaaattg
    31561 ccagcgcatg gctgtcgtca tgttgcgatc attatggacg gcaatggtcg ctgggcaaaa
    31621 aagcaaggga agattcgtgc ctttgggcat aaagccgggg caaaatccgt ccgccgggct
    31681 gtctcttttg cggccaacaa cggtattgag gcgttaacgc tgtatgcctt tagtagtgaa
    31741 aactggaacc gaccagcgca ggaagtcagt gcgttaatgg aactgtttgt gtgggcgcta
    31801 gatagcgaag taaaaagtct gcaccgacat aacgtgcgtc tgcgtattat tggcgatacc
    31861 agtcgcttta actcgcgttt gcaagaacgt attcgtaaat ctgaagcgct aacagccggg
    31921 aataccggtc tgacgctgaa tattgcggcg aactacggtg gacgttggga tatagtccag
    31981 ggagtcaggc aactggctga aaaggtgcag caaggaaacc tgcaaccaga tcagatagat
    32041 gaagagatgc taaaccagca tgtctgtatg catgaactgg cccctgtaga tttagtaatt
    32101 aggactgggg gggagcatcg cattagtaac tttttgcttt ggcaaattgc ctatgccgaa
    32161 ctttacttta cagatgttct ctggcccgat ttcgatgaac aagactttga aggggcgtta
    32221 aatgcctttg ctaatcgaga gcgtcgtttc ggcggcaccg agcccggtga tgaaacagcc
    32281 tgatgggggt cgcttttgct gaagtatcgc ctgatatctg cttttgtgtt aatacccgtc
    32341 gtcatcgcgg cgttgtttct gttgccgccg gtggggttcg ccattgtaac gctggtggtc
    32401 tgtatgctgg cagcgtggga atggggacag cttagcggtt ttaccactcg ttcgcagcga
    32461 gtatggttgg cggtgttatg cgggttattg ttggcgctga tgctttttct gttgccggaa
    32521 tatcaccgaa atattcatca accgctggtt gaaatctcac tttgggcttc gctgggttgg
    32581 tggattgtcg cgctattgct ggtgctgttt tacccaggtt ccgcagcaat ctggcgtaac
    32641 tctaaaacat tgcgcattat ttttggcgtg ctaaccattg ttcccttctt ctggggcatg
    32701 ctggcgttac gggcctggca ctacgacgag aatcattaca gtggcgcaat atggctgctc
    32761 tatgtcatga tcctggtatg gggcgctgac tctggcgcat atatgtttgg caaattgttt
    32821 ggtaaacata aactggcacc gaaggtttct ccgggtaaaa cctggcaagg ctttatcggt
    32881 ggactcgtga ccgcagcggt aatctcatgg ggttatggca tgtgggtgaa tctcgacgtc
    32941 gctcccgtca ccttactcat ttgctctatt gtcgcagcgt tagcctcagt gctcggcgat
    33001 ctgaccgaaa gtatgtttaa gcgtgaagcg ggaattaagg acagcggtca tttaattcca
    33061 ggacacggtg gtattttaga tcgtattgat agcctgacgg ctgcggtacc ggtctttgct
    33121 tgcttgttgt tactggtatt caggacgctt taacggaagg taatatgctg agttttctct
    33181 gggatttggc ttcgttcatc gttgcactgg gtgtacttat caccgtgcat gaatttggtc
    33241 atttctgggt tgcccggcgt tgtggtgttc gcgttgagcg tttctcaata gggtttggta
    33301 aggcgctctg gcggcgaact gataagctcg gcaccgaata tgttatcgcc ctgatcccgt
    33361 tgggcggtta tgtcaaaatg ctggatgagc gcgcagaacc ggtcgttccg gaactccgcc
    33421 accatgcctt caataataaa tctgtcggcc aacgagcggc gattattgcc gcaggtccag
    33481 ttgcaaactt catttttgct atctttgcct actggctggt ttttattatt ggtgtgcctg
    33541 gcgtacgtcc ggtggttggt gaaatagcag ccaattcgat agctgcggaa gcacaaattg
    33601 caccaggtac ggaactaaaa gccgtagatg gtatcgaaac gcctgattgg gatgccgtgc
    33661 gtttgcagtt ggtcgataaa attggcgatg aaagcaccac cattacggta gcgccatttg
    33721 gcagcgacca acggcgggat gtaaagctcg atttacgtca ctgggcgttt gagcctgata
    33781 aagaagatcc ggtatcttcg ttggggattc gtcctcgtgg gccgcaaatt gaacctgtac
    33841 tggaaaatgt gcagccaaac tcggcggcaa gcaaggcagg tttgcaagca ggcgacagga
    33901 tcgttaaagt cgatggtcag cccttaacgc agtgggtgac ctttgtgatg cttgtccggg
    33961 ataacccggg taaatcctta gcgttagaaa tcgaaagaca ggggagtccc ttgtctttga
    34021 cattaatccc ggagagtaaa ccgggtaatg gtaaagcgat tggttttgtc ggtattgagc
    34081 cgaaagtcat tcctttgcct gatgagtata aagttgtacg ccagtatggg ccgttcaacg
    34141 ctatcgtcga agccacggac aaaacgtggc agttgatgaa gctgacggtc agtatgctgg
    34201 gaaaattgat caccggtgat gtgaaactga acaacctcag tgggccgatc tctatcgcca
    34261 agggggctgg gatgacagcg gaactcgggg tagtttatta cctgccgttt cttgcgctta
    34321 ttagcgtgaa cttagggata attaacctgt ttccgttgcc cgtacttgac ggggggcatc
    34381 tgctgttcct tgcgatcgaa aagatcaagg gcggaccggt atccgagcgg gttcaagact
    34441 tttgttatcg cattggctcg attctgctgg tgctgttaat ggggcttgca cttttcaatg
    34501 atttctctcg gttatgagag ttagttagga agaacgcata ataacgatgg cgatgaaaaa
    34561 gttgctcata gcgtcgctgc tgtttagcag cgccaccgta tacggtgctg aagggttcgt
    34621 agtgaaagat attcatttcg aaggccttca gcgtgtcgcc gttggtgcgg ccctcctcag
    34681 tatgccggtg cgcacaggcg acacggttaa tgatgaagat atcagtaata ccattcgcgc
    34741 tctgtttgct accggcaact ttgaggatgt tcgcgtcctt cgtgatggtg atacccttct
    34801 ggttcaggta aaagaacgtc cgaccattgc cagcattact ttctccggta acaaatcggt
    34861 gaaagatgac atgctgaagc aaaacctcga ggcttctggt gtgcgtgtgg gcgaatccct
    34921 cgatcgcacc accattgccg atatcgagaa aggtctggaa gacttctact acagcgtcgg
    34981 taaatatagc gccagcgtta aagctgtcgt gaccccgctg ccgcgcaacc gtgttgacct
    35041 aaaactggtg ttccaggaag gtgtgtcagc tgaaatccag caaattaaca ttgttggtaa
    35101 ccatgctttc accaccgatg aactgatctc tcatttccaa ctgcgtgacg aagtgccgtg
    35161 gtggaacgtg gtaggcgatc gtaaatacca gaaacagaaa ctggcgggcg accttgaaac
    35221 cctgcgcagt tactatctgg atcgcggtta tgcccgtttc aacatcgatt ctacccaggt
    35281 cagtctgacg ccagataaaa aaggtattta cgtcacggtg aacatcaccg aaggcgatca
    35341 gtacaagctt tctggcgttg aagtgagcgg caatcttgcc gggcactccg ctgaaattga
    35401 gcaactgact aagatcgagc cgggcgagct gtataacggc accaaagtga ccaaaatgga
    35461 agatgacatt aaaaagcttc ttggtcgcta tggttatgcc tatccgcgcg tacagtcgat
    35521 gcctgaaatt aacgatgccg acaaaaccgt taaattacgc gtgaacgttg atgcgggtaa
    35581 ccgtttctat gtgcgtaaga tccgttttga aggtaacgat acctcgaaag atgctgtcct
    35641 gcgtcgcgaa atgcgtcaga tggaaggtgc atggctgggg agcgatctgg tcgatcaggg
    35701 taaggagcgt ctgaaccgtc tgggcttctt tgaaactgtc gataccgata cccaacgtgt
    35761 tccggggagc ccggatcagg ttgatgtagt ttacaaggtg aaagaacgta acaccggtag
    35821 cttcaacttc gggattggtt acggtactga aagtggcgtg agcttccagg ctggtgtgca
    35881 acaggataac tggttaggta cgggttatgc tgttggtatc aacgggacca aaaacgatta
    35941 ccagacctat gctgaactgt cggtaaccaa cccgtacttc accgtagatg gcgtaagcct
    36001 cggtggtcgt ctcttctata atgacttcca ggcagatgac gccgacctgt ccgactatac
    36061 caacaagagt tatggtacag acgtgacgtt gggcttcccg atcaacgaat ataactcgct
    36121 gcgtgcaggt ctgggttatg tacataactc cctgtccaac atgcagcctc aggttgcgat
    36181 gtggcgttat ctgtactcta tgggtgaaca tccgagcacc tctgatcagg ataacagctt
    36241 caaaacggac gacttcacgt tcaactatgg ttggacctat aacaagcttg accgtggtta
    36301 cttcccgaca gatggttcac gtgtcaacct gaccggtaaa gtgaccattc cgggatcgga
    36361 taacgaatac tacaaagtga cgttagacac ggcgacttat gtgccgattg atgacgatca
    36421 caaatgggtt gttctgggtc gtacccgttg gggttatggt gatggtttag gcggcaaaga
    36481 gatgccgttc tatgagaact tctatgccgg tggttccagc accgtgcgtg gcttccagtc
    36541 caataccatt ggtccgaaag cagtttactt cccgcatcag gccagtaatt atgatccgga
    36601 ctatgattac gaatgtgcga ctcaggacgg cgcgaaagat ctgtgtaaat cggatgatgc
    36661 tgtaggcggt aacgccatgg cggttgccag cctcgagttc atcaccccga cgccgtttat
    36721 tagcgataag tatgctaact cggttcgtac ttccttcttc tgggatatgg gtaccgtttg
    36781 ggatacaaac tgggattcca gccaatattc tggatatccg gactatagtg atccaagcaa
    36841 tatccgtatg tctgcgggta tcgcattaca atggatgtcc ccattggggc cgttggtgtt
    36901 ctcctacgcc cagccgttca aaaagtacga tggagacaag gcagaacagt tccagtttaa
    36961 catcggtaaa acctggtaag tgttctccac aaaggaatgt agtggtagtg tagcgatgac
    37021 tttaggcgat caatataaga tcgccgggcc acgcaaagaa ctgcaccctc cggtgcaaat
    37081 gggatggtaa ggagtttatt gtgaaaaagt ggttattagc tgcaggtctc ggtttagcac
    37141 tggcaacttc tgctcaggcg gctgacaaaa ttgcaatcgt caatatgggc agcctgttcc
    37201 agcaggtagc gcagaaaacc ggtgtttcta acacgctgga aaacgagttc aaaggccgtg
    37261 ccagcgaact gcagcgtatg gaaaccgatc tgcaggctaa aatgaaaaag ctgcagtcca
    37321 tgaaagcggg cagcgatcgc actaagctgg aaaaagacgt gatggctcag cgccagactt
    37381 ttgctcagaa agcgcaggct tttgagcagg atcgcgcacg tcgttccaac gaagaacgcg
    37441 gcaaactggt tactcgtatc cagactgctg tgaaatccgt tgccaacagc caggatatcg
    37501 atctggttgt tgatgcaaac gccgttgctt acaacagcag cgatgtaaaa gacatcactg
    37561 ccgacgtact gaaacaggtt aaataagtaa tgccttcaat tcgactggct gatttagcgc
    37621 agcagttgga tgcagaacta cacggtgatg gcgatatcgt catcaccggc gttgcgtcca
    37681 tgcaatctgc acaaacaggt cacattacgt tcatggttaa cccaaaatac cgtgagcatt
    37741 taggcttgtg ccaggcgtcc gcggttgtca tgacccagga cgatcttcct ttcgcgaaaa
    37801 gtgccgcgct ggtagtgaag aatccctacc tgacttacgc gcgcatggcg caaattttag
    37861 ataccacgcc gcagcccgcg cagaacattg cacccagtgc ggtgattgac gcgacggcga
    37921 agctgggtaa caacgtatcg attggcgcta acgcggtgat tgagtccggc gttgaactgg
    37981 gcgataacgt gattatcggt gccggttgct tcgtaggtaa aaacagcaaa atcggtgcag
    38041 gttcgcgtct ctgggcgaac gtaaccattt accatgagat ccagatcggt cagaattgcc
    38101 tgatccagtc cggaacagtg gtaggcgcag acggctttgg ttatgccaac gatcgtggta
    38161 actgggtgaa gatcccacag attggtcggg taattattgg cgatcgcgtg gagatcggtg
    38221 cctgcactac tattgatcgt ggcgcgctgg atgacactgt tattggcaat ggtgtgatca
    38281 ttgataacca gtgccagatt gcacataacg tcgtgattgg cgacaatacg gcagttgccg
    38341 gtggcgtcat tatggcgggc agcctgaaaa ttggtcgtta ctgcatgatc ggcggagcca
    38401 gcgtaatcaa cgggcatatg gaaatatgcg acaaagtgac ggttacgggc atgggtatgg
    38461 tgatgcgtcc catcactgaa ccaggcgtct attcctcagg cattccgctg caacccaaca
    38521 aagtctggcg caaaaccgct gcactggtga tgaacattga tgacatgagc aagcgtctga
    38581 aatcgcttga gcgcaaggtt aatcaacaag actaacgttc catcttttgt tcgccaaact
    38641 ttacggcctg tctcattctt acgattgcgg caggccgtgt tattattgtc gtttcttata
    38701 ttttgacagg aagagtatct tgactactaa cactcatact ctgcagattg aagagatttt
    38761 agaacttctg ccgcaccgtt tcccgttctt actggtggat cgcgtgctgg attttgaaga
    38821 aggtcgtttt ctgcgcgcag taaaaaatgt ctctgtcaat gagccattct tccagggcca
    38881 tttccctgga aaaccgattt tcccgggtgt gctgattctg gaagcaatgg cacaggcaac
    38941 aggtattctg gcgtttaaaa gcgtaggaaa actggaaccg ggtgagctgt actacttcgc
    39001 cggtattgac gaagcgcgct ttaagcgccc ggtcgtgcct ggcgatcaaa tgatcatgga
    39061 agtcactttc gaaaaaacgc gccgcggcct gacccgtttt aaaggggttg ctctggtcga
    39121 cggtaaagta gtttgcgaag caacgatgat gtgtgctcgt agccgggagg cctgatacgt
    39181 gattgataaa tccgcctttg tgcatccaac cgccattgtg gaagagggcg cgtcgattgg
    39241 cgcgaacgct cacattggtc ctttttgtat cgttggaccc catgtcgaaa ttggtgaggg
    39301 taccgtactg aaatctcacg ttgtcgtgaa tggtcatact aaaattggcc gcgataatga
    39361 gatttatcag ttcgcctcca tcggcgaagt taaccaggat ctgaaatatg ctggcgaacc
    39421 gacccgcgtg gaaatcggcg atcgtaaccg cattcgcgaa agcgtcacca ttcatcgtgg
    39481 cacagtccag ggcggtggat tgacgaaggt gggcagcgac aacttactga tgatcaacgc
    39541 gcacattgcg cacgattgta cggtaggtaa ccgctgcatc ctcgccaaca acgcaacgct
    39601 ggcgggtcac gtatcggttg acgacttcgc gatcatcggc ggcatgaccg cagtccatca
    39661 gttctgcatc attggtgcgc atgtgatggt tggcggctgc tccggtgtgg cgcaggacgt
    39721 ccctccttat gtcattgcgc agggtaacca cgcaacgccg ttcggtgtca atatcgaagg
    39781 gctgaagcgc cgcggattca gccgtgaggc gattaccgct atccgcaatg cgtataagct
    39841 gatttatcgt agcggtaaga cgctcgatga agtgaaaccg gaaattgctg aactggcgga
    39901 aacatatccg gaagtgaaag cctttaccga tttctttgca cgctcaacgc gcggtctgat
    39961 tcgttaatga ctgaacagcg tccattaacg attgccctgg tcgccggaga aacctccggc
    40021 gatatcctgg gggccggttt aatccgcgct ctgaaagaac gtgtgcccaa cgcccgcttt
    40081 gttggtgttg ccgggccacg aatgcaggct gaaggctgcg aagcctggta cgaaatggaa
    40141 gaactggcag tgatgggcat tgttgaagtg ctcggtcgtc tgcgtcgctt actacatatt
    40201 cgtgccgatc tgacaaagcg ttttggcgaa ctgaagccag atgtttttgt tggtattgat
    40261 gcgcctgact tcaatattac tcttgaaggt aacctcaaaa agcagggtat caaaaccatt
    40321 cattacgtca gtccgtccgt ctgggcgtgg cgacagaaac gcgttttcaa aataggcaga
    40381 gccaccgatc tggtgctcgc atttctgcct ttcgaaaaag cgttttatga caaatacaac
    40441 gtaccgtgcc gctttatcgg tcataccatg gctgatgcca tgccattaga tccagataaa
    40501 aatggtgccc gtgatgtgct ggggatccct tacgatgccc actgtctggc attgttgccg
    40561 ggcagccgtg gtgcagaagt cgaaatgctt agtgccgatt tcctgaaaac ggcccagctt
    40621 ttgcgccaga catatccgga tctcgaaatc gtggtgccgc tggtgaatgc caaacgccgt
    40681 gagcagtttg aacgcatcaa agctgcagtc gcgccagacc tgtcggttca tttgctggat
    40741 ggaatgggcc gtgaggcgat ggtcgccagt gatgcggcgc tactggcgtc gggtacggca
    40801 gcactggagt gtatgctggc taaatgcccg atggtagtgg gatatcgcat gaagcctttc
    40861 accttctggc tggcgaagcg actggtgaaa actgattatg tctcgctgcc aaatctgctg
    40921 gcgggcagag agttagtcaa agagttattg caggaagagt gtgagccgca aaaattggct
    40981 gcggcgctgt taccgctgtt ggcgaacggg aaaaccagcc atgcgatgca cgacaccttc
    41041 cgcgagctgc atcagcagat ccgctgcaat gccgatgagc aggcggcaca agccgttctg
    41101 gagttagcac aatgatcgaa tttgtctatc cgcacacgca gctggttgcg ggtgtggatg
    41161 aagtcggacg cgggccgtta gttggcgcgg tcgtcaccgc tgcggtgatc cttgacccgg
    41221 cgcgcccgat tgccgggctg aatgattcca aaaagctgag cgaaaaacgc cgtctggcgc
    41281 tctgtgaaga gatcaaagag aaagcgttga gctggagtct gggccgcgcg gaacctcacg
    41341 aaatcgacga actgaatatt ttacatgcga ccatgctggc gatgcagcgt gccgtcgctg
    41401 gactgcatat tgcgccggaa tatgtgttga ttgatggtaa ccgctgcccg aaattaccga
    41461 tgccttcgat ggctgtggtg aaaggcgata gccgcgtacc ggaaatcagt gccgcgtcta
    41521 tcctggcgaa agtgacgcgt gacgccgaaa tggcggcgct ggatattgtt ttcccgcaat
    41581 atggttttgc ccaacacaaa gggtatccaa ccgcttttca tctggaaaaa ctggctgaac
    41641 acggcgcgac cgaacaccat cggcgcagct ttgggcctgt caaacgcgca ctgggacttg
    41701 cgtcctgatt cttgtgtcga gattaagtaa accggaatct gaagatgtct gaaccacgtt
    41761 tcgtacacct gcgggtgcac agtgactact cgatgatcga tggcctggcc aaaaccgcac
    41821 cgctggtaaa aaaggcggca gcgttgggta tgccagcact ggcgatcacc gatttcacca
    41881 acctttgcgg tctggtgaag ttctacggag cgggacatgg cgcagggatt aagcccatcg
    41941 tcggggcgga ttttaacgtc cagtgcgacc tgctgggtga tgagttaact cacctgacgg
    42001 tgctggcggc gaacaatacc ggctatcaga atctgacgtt gctgatctca aaagcgtatc
    42061 agcgcgggta cggtgccgcc gggccgatca tcgatcgcga ctggcttatc gaattaaacg
    42121 aagggttgat ccttctttcc ggcgggcgca tgggcgacgt tggacgcagt cttttgcgtg
    42181 gtaacagcgc gctggtagat gagtgtgtcg cgttttatga agaacacttc ccggatcgct
    42241 attttctcga gctgatccgc accggcaggc cggacgaaga aagctatctg cacgctgcgg
    42301 tggaactggc ggaatcacgc ggtttgcccg ttgtggcgac caacgacgtg cgctttatcg
    42361 acagcagcga ctttgacgca cacgaaatcc gcgtcgcgat ccacgacggc tttaccctcg
    42421 acgatcctaa acgcccgcgt aactattcgc cgcagcaata tatgcgtagc gaagaggaga
    42481 tgtgcgagct ttttgccgac attcccgaag cccttgccaa caccgttgag atagccaaac
    42541 gttgtaacgt aactgtgcgt ctgggtgaat acttcttgcc gcaattcccg accggggaca
    42601 tgagtaccga agattatctg gtcaagcgtg caaaagaagg cctggaagag cgtctggcat
    42661 ttttattccc tgacgaggaa gaacgtctta agcgtcgtcc ggagtatgac gaacgtctgg
    42721 atactgaact tcaggttatc aaccagatgg gcttcccggg ttacttcctc atcgttatgg
    42781 aatttatcca gtggtcgaaa gataacggtg taccggtagg gccaggccgt ggttctggtg
    42841 cagggtcact ggtggcctac gcgttaaaaa tcaccgatct cgatccgctg gaatttgacc
    42901 tgctgttcga acgtttcctt aacccggaac gtgtctccat gcctgacttc gacgttgact
    42961 tctgtatgga gaaacgtgac caggttatcg aacatgtggc ggacatgtac ggtcgcgatg
    43021 cggtatcgca gattatcacc ttcggtacga tggcggcgaa agcggtgatc cgcgacgtag
    43081 gccgtgtatt ggggcatccg tacggatttg tcgatcgtat ctcgaaactg atcccgcccg
    43141 atccgggtat gacgctggcg aaagcgtttg aagccgagcc gcagctgccg gaaatctacg
    43201 aagcggatga agaagttaag gcgctgatcg acatggcgcg caaactggaa ggggtcaccc
    43261 gtaacgccgg taagcacgcc ggtggggtgg ttatcgcgcc gaccaaaatt actgattttg
    43321 cgccgcttta ttgcgatgaa gagggcaaac atccggtcac ccagtttgat aaaagcgacg
    43381 ttgaatacgc cgggctggtg aagttcgact tcctcggttt gcgtacgctc accatcatca
    43441 actgggcgct ggagatgatc aacaagcggc gggcgaagaa tggcgagccg ccgctggata
    43501 tcgccgcgat cccgctggat gacaagaaaa gcttcgacat gctgcaacgc tcggaaacca
    43561 cggcggtatt ccagcttgaa tcgcgcggca tgaaagacct gatcaagcgt ctgcaacctg
    43621 actgcttcga agatatgatc gcactggtgg cactgttccg ccccggcccg ttgcaatcag
    43681 ggatggtgga taactttatc gaccgtaaac atggtcgcga agagatctcc tatccggacg
    43741 tgcagtggca gcatgaaagc ctgaaaccgg tactggagcc aacctacggc atcatcctgt
    43801 atcaggaaca ggtcatgcag attgcccagg tgctttctgg ttataccctc ggtggcgcgg
    43861 atatgctgcg tcgtgcgatg ggtaagaaaa agccggaaga gatggctaag cagcgttctg
    43921 tatttgctga aggtgcagaa aagaacggaa tcaacgccga actggcgatg aaaatcttcg
    43981 acctggtgga gaaattcgcg ggttacggat ttaacaaatc gcactcggcg gcctatgctt
    44041 tggtgtcata tcaaacgtta tggctgaagg cacattatcc ggcagagttt atggcggcgg
    44101 taatgaccgc cgatatggac aacaccgaga aggtggtggg cctggtggat gagtgctggc
    44161 ggatggggct gaaaatcctg ccaccagata taaactccgg tctttaccat ttccacgtca
    44221 acgacgacgg cgaaatcgtg tatggtattg gcgcgatcaa aggggtaggt gaaggcccga
    44281 ttgaggccat catcgaagcc cgtaataaag gcggctactt ccgcgaactg tttgatctct
    44341 gcgcccgtac cgacaccaaa aagttaaacc ggcgggtgct ggaaaaactg atcatgtccg
    44401 gggcgtttga ccgtcttggg ccacaccgcg cggcgctgat gaactcgctg ggcgatgcgt
    44461 taaaagcggc agatcaacac gcgaaagcgg aagctatcgg tcaggccgat atgttcggcg
    44521 tgctggccga agagccggaa caaattgaac aatcctacgc cagctgccaa ccgtggccgg
    44581 agcaggtggt gttagatggg gaacgtgaaa cgttaggtct gtacctcacg ggacacccta
    44641 tcaaccagta tttaaaagag attgagcgtt atgtcggagg cgtaaggctg aaagacatgc
    44701 acccgacaga acgtggtaaa gtcatcacgg ctgcggggct cgttgttgcc gcgcgggtta
    44761 tggtcaccaa gcgcggcaat cgtatcggta tctgcacgct ggatgaccgt tccgggcggc
    44821 tggaagtgat gttgtttact gacgccctgg ataaatacca gcaattgctg gaaaaagacc
    44881 gcatacttat cgtcagcgga caggtcagct ttgatgactt cagcggtggg cttaaaatga
    44941 ctgctcgcga agtgatggat attgacgaag cccgggaaaa atatgctcgc gggcttgcta
    45001 tctcgctgac ggacaggcaa attgatgacc agcttttaaa ccgactccgt cagtctctgg
    45061 aaccccaccg ctctgggaca attccagtac atctctacta tcagagggcg gatgcacgcg
    45121 cgcgattgcg ttttggcgcg acgtggcgtg tctctccgag cgatcgttta ttaaacgatc
    45181 tccgtggcct cattggttcg gagcaggtgg aactggagtt tgactaatac aggaatacta
    45241 tgagtctgaa tttccttgat tttgaacagc cgattgcaga gctggaagcg aaaatcgatt
    45301 ctctgactgc ggttagccgt caggatgaga aactggatat taacatcgat gaagaagtgc
    45361 atcgtctgcg tgaaaaaagc gtagaactga cgcgtaaaat cttcgccgat ctcggtgcat
    45421 ggcagattgc gcaactggca cgccatccac agcgtcctta taccctggat tacgttcgcc
    45481 tggcatttga tgaatttgac gaactggctg gcgaccgcgc gtatgcagac gataaagcta
    45541 tcgtcggtgg tatcgcccgt ctcgatggtc gtccggtgat gatcattggt catcaaaaag
    45601 gtcgtgaaac caaagaaaaa attcgccgta actttggtat gccagcgcca gaaggttacc
    45661 gcaaagcact gcgtctgatg caaatggctg aacgttttaa gatgcctatc atcaccttta
    45721 tcgacacccc gggggcatat ccgggcgtgg gcgcagaaga acgtggtcag tcggaagcca
    45781 ttgcacgcaa cctgcgtgaa atgtctcgcc tcggcgtacc ggtagtttgt acggttatcg
    45841 gtgaaggtgg ttctggtggt gcgctggcga ttggcgtggg cgataaagtg aatatgctgc
    45901 aatacagcac ctattccgtt atctcgccgg aaggttgtgc gtccattctg tggaagagcg
    45961 ctgacaaagc gccgctggcg gctgaagcga tgggtatcat cgctccgcgt ctgaaagagc
    46021 tgaaactgat cgactccatc atcccggaac cgctgggcgg tgctcaccgt aacccggaag
    46081 cgatggcggc gtctctgaaa gcgcagctgc tggcggatct ggccgatctc gacgtgttaa
    46141 gcactgaaga tttaaaaaat cgtcgttatc agcgcctgat gagctacggc tacgcgtaat
    46201 cggcaaatgt tctgaaaagg gtcacttcgg tggccctttt ttatcgccac ggtttgagca
    46261 ggctatgatt aaggaaggat tttccaggag gaacacatga acatcattgc cattatggga
    46321 ccgcatggcg tcttttataa agatgagccc atcaaagaac tggagtcggc gctggtggcg
    46381 caaggctttc agattatctg gccacaaaac agcgttgatt tgctgaagtt tatcgaacat
    46441 aacccacgaa tttgcggcgt gatttttgac tgggatgagt acagtctcga tttatgtagc
    46501 gatatcaatc aacttaatga atatctcccg ctttatgcct tcatcaacac ccactcgacg
    46561 atggatgtca gcgtgcagga tatgcggatg gcgctctggt tttttgaata tgcgctgggg
    46621 caggcggaag atatcgccat tcgtatgcgt cagtacacca acgaatatct tgataacatt
    46681 acgccgccgt ttacgaaagc cttgtttacc tacgtcaaag agcggaagta caccttttgt
    46741 acgccggggc atatgggcgg taccgcatat caaaaaagcc cggttggctg tctgttttat
    46801 gattttttcg gcgggaatac cctcaaggcg gacgtctcta tttcggtcac cgagcttggt
    46861 tcgttgctcg accacaccgg gccacacctg gaagcggaag agtacatcgc gcggacgttt
    46921 ggcgcggaac agagttatat cgttactaat ggaacatcga cgtcgaacaa aattgtgggt
    46981 atgtacgccg cgccatccgg cagtacgctg ttgatcgacc gcaattgtca taaatcgctg
    47041 gcgcatctgt tgatgatgaa cgatgtagtg ccagtctggc tgaaaccgac gcgtaatgcg
    47101 ttggggattc tgggtgggat cccgcgcggt gaatttactc gcgacagcat cgaagagaaa
    47161 gtcgctgcca ccacgcaggc acaatggccg gttcatgcgg tgatcaccaa ctccacctat
    47221 gatggcttgc tctacaacac cgactggatc aaacagacgc tggatgtccc gtcgattcac
    47281 ttcgattctg cctgggtgcc gtacacccat tttcatccga tctaccaggg taaaagtggt
    47341 atgagcggtg agcgtgttgc gggaaaagtg atcttcgaaa cgcagtcgac ccacaaaatg
    47401 ctggcggcgt tgtcccaggc gtcgctgatc cacattaaag gcgagtatga cgaagaggcg
    47461 tttaacgaag cctttatgat gcataccacc acctcgccca gttatcccat tgttgcttcg
    47521 gttgagacgg cggcggcgat gctgcgtggt aatccgggca aacggctgat taaccgttca
    47581 gtagaacgag cgctgcattt tcgcaaagag gtccagcggc tgcgggaaga gtctgacagc
    47641 tggtttttcg atatctggca accgccgcag gtggatgaag ccgaatgctg gcacgttgcg
    47701 cctggcgaac agtggcacgg ctttagcgat gcggatgcca atcacatgtt tctcgatccg
    47761 gttaaagtca ctattttgac accgggaatg gacgagcagg gcaatatgag cgaggagggg
    47821 atcccggcgg cgctggtggc aaaattcctc gacgaacgtg ggatcgtagt agagaaaacc
    47881 ggcccttata acctgctgtt tctctttagt attggcatcg ataaaaccaa agcaatggga
    47941 ttattgcgtg ggttgacgga atttaagcgc tcttacgatc tcaacctgcg gatcaaaaat
    48001 atgctgcccg atctctatgc agaagatccc gatttctacc gcaatatgcg tattcaggat
    48061 ctggcgcaag ggatccataa gctgattcgt aaacacgatc ttcccggttt gatgttgcgg
    48121 gcatttgata ccttgccgga gatgatcatg acgccacatc aggcatggca gagacagatt
    48181 aaaggcgaag tagaaaccat tgcgctggaa caactggtcg gtagagtatc ggcaaatatg
    48241 atcctgcctt atccacccgg cgtaccgctg ctgatgccgg gagaaatgct taccgaagag
    48301 agccgcacgg tactcgattt tctactgatg ctttgttccg tagggcaaca ttaccccggt
    48361 tttgaaacgg atattcacgg cgcgaaacag gacgaagacg gcgtttaccg cgtacgagtc
    48421 ctaaaaatgg cgggataact tgccagagcg gcttccgggc gagtaacgtg ctgttaacaa
    48481 ataaaggaga cgttatgctg ggtttaaaac aggttcacca tattgcgatt attgcgacgg
    48541 attatgcgat gagcaaagct ttctactgcg atattcttgg tttcacgctg caaagcgaag
    48601 tctatcgcga agcgcgcgac tcgtggaaag gggatttggc gcttaatggg caatatgtga
    48661 ttgagctttt ctcatttccg ttcccgccgg agcgccccag ccgaccggaa gcttgcggcc
    48721 tgcgtcatct ggcttttagc gttgatgata tcgatgcggc agtggcgcac cttgaaagcc
    48781 ataacgtgaa gtgtgaagcc atccgtgtcg atccatacac gcaaaaacgc ttcacgttct
    48841 ttaacgatcc ggacgggctg ccgttggaac tgtatgagca gtaaggcttg tcatcgccgc
    48901 atttgcccgg taacgtgccg ggcattgcta ctgtaaaatc gcaccatcat gacactcacg
    48961 ctcaatagac aacttctctc ctcacgccag attctggtgg cctttagcgg cggccttgat
    49021 tctactgtct tactgcatca actggtgcag tggcggacgg aaaatccggg cgtcactctg
    49081 cgcgctatcc atgtgcatca cggtttaagc gccaatgctg atgcctgggt gaaacattgt
    49141 gaaaacatct gccaacagtg gcaggtgccg ctggtggtcg aacgtgtgca gcttgcgcaa
    49201 gaggggctag gtattgaggc ccaggcgcgg caggcacgtt atcaggcatt tgcccgtacc
    49261 ttgttgcccg gtgaagtgct ggttacagcg caacatctcg acgatcaatg tgaaaccttt
    49321 ctgctggcgc taaaacgcgg tagcggccct gccgggcttt cggctatggc ggaagtttcg
    49381 gagtttgccg gaacacggct tatccgcccg ttgcttgccc gcacgcgggg ggaactggag
    49441 cagtgggcgc gtcagtatga tttacgctgg attgaagacg aaagtaatca agacgattcc
    49501 tacgatcgta acttcttgcg cctgcgcgta gtgccgttat tgcagcagcg ttggccgcat
    49561 tttgccgaag cgacggcccg cagcgccgca ctttgcgccg aacaagagag cctgctggat
    49621 gaactgctgg cggatgattt agcgcactgc caaacgcaac agggaacgtt aaagattgcg
    49681 ccaatgctgg caatgagcga tgcccgtcgc gcggcgatta tccgccgctg gctggcaggg
    49741 cagaatgcac ctatgccttc ccgcgaggcg ctggtgcgga tctggcagga agtggcgctg
    49801 gcgcgggaag atgcttcccc ctgtttacgt ttgggcgcgt ttgaaatccg ccgctatcag
    49861 tcgcaactgt ggtggattaa atccgttgcc gggcaaagcg aaaccattgt gccgtggcag
    49921 acgtggctac aaccgctgga attaccggcg gggctgggaa gtgtacagct tactgcggga
    49981 ggcgatattc gccctccgcg tgcagacgaa gcggtcagcg tgcgtttcaa agcgccagga
    50041 ctgctgcata ttgtcgggcg caacggtgga cgtaagctga agaaaatctg gcaagagtta
    50101 ggcgttccgc cgtggctacg tgacaccacg ccactgctgt tttatggcga aacgctgatt
    50161 gcggcggcag gggtatttgt gacgcaagaa ggtgtggctg taggtgagaa tggcgtcagt
    50221 tttgtctggc agaaaacgct tagtcaagtg aaagccggat aagacgcatc aaacgtcgca
    50281 tccggcgaaa gtcaatcagg actcgctcac caccaccgta ccgatttccg ggtggctaaa
    50341 gctggtaatt ttatccagac gcagctcgcg ggttgcacca gcggcctcga cgaccagata
    50401 ctccacattt ttgcgggaga ctaaatcgct ggccttcgcc tgcaattttt cgccatcttt
    50461 cagctcaagt gtcagcatta aatgatgctg gcaggcgagc tcaagattat cgtaatcatc
    50521 acaattgatt ggttgatacg tatcattcat tgacataatc gctcaccagt aagtttgccg
    50581 cagcgtatgc tgctttttcc ctgacagcct cagaaagggc gtcgtcggca gccatttcat
    50641 tcagcacttt caaaacgcag cccagcgcgt ccggaacgta tcctaagtct ccgctggcga
    50701 tttccgcgta ccgcttgcgt attaactcac aatatttttc cacatgccct cctgtcagca
    50761 ctctgactta accgtggatg caagtctaag cctacgaaga taaactctgt ttcgcaaggt
    50821 gactatacca cactcatttc tgcaatatca gcgccgcaac tgcacgtatt ccgttacaat
    50881 ggcctcctga ttcgaaagga gttttcttat ggcgcttaaa gcgacaattt ataaagcgac
    50941 ggttaatgtg gccgatctcg accgcaacca gtttctcgat gcctctctga cgttggcgcg
    51001 ccatccttca gaaacccagg agcgtatgat gctgcgcctg ctggcgtggc taaaatatgc
    51061 cgatgaacgt ctgcaattta cccgtggttt gtgtgccgat gatgagccgg aagcgtggct
    51121 gcgtaacgat catttgggta ttgatttgtg gattgagctg gggctgccgg atgagcgacg
    51181 gattaagaaa gcctgcaccc aggccgcaga agtggcgctg tttgcctata atagtcgggc
    51241 ggcgcaaatc tggtggcagc aaaatcagag caaatgtgcg cagtttgcca atctttccgt
    51301 ctggtatctg gacgacgagc agctggcgaa agtgagcgcc tttgccgatc gtaccatgac
    51361 gctgcaggca acgattcagg atggtgttat ctggttatcg gatgataaga ataatctgga
    51421 agtgaactta accgtctggc aacaaccttc atgattgtga tttcccgaca tgttgctatc
    51481 cctgatggcg agtttgagat caccgccatt cgtgcgcagg gcgcgggcgg gcagcatgtt
    51541 aataagacct caacggctat tcatctgcgt tttgacattc gggcgtccag cctgccagag
    51601 tattacaaag agcgtctgct cgccgccagc catcatttga tcagcagtga cggagtgatt
    51661 gtcattaagg cacaggaata ccgcagtcag gaactgaacc gcgaagcggc gctggcccgg
    51721 ctggtggcag tgattaaaga tttaacaaca gaacaaaaag cccgaagacc tacgcggccc
    51781 acccgtgcat cgaaagagcg caggctggca tcgaaagcac aaaaatcaag cgtgaaggcg
    51841 atgcgcggca aagtgcgcag cggtcgggag taaaaagaag gaatggatgg tgaaaaaagc
    51901 gatagtgaca gcgatggctg taatcagcct ttttactctg atgggatgta ataatcgggc
    51961 cgaagtcgat acgctttccc cggcgcaggc tgccgaactg aaaccgatgc cgcaaagttg
    52021 gcgcggcgtg ctgccgtgtg ctgattgcga aggaatcgaa acctctctgt tcctcgaaaa
    52081 agatggaacc tgggtgatga atgaacgtta tctgggggca cgtgaagagc cttcctcttt
    52141 cgcttcctac ggtacatggg cgcgaactgc cgacaagctg gtattaaccg acagcaaagg
    52201 tgaaaagtca tattatcgag cgaaagggga tgcactggag atgctcgatc gtgaaggtaa
    52261 tcccattgaa tcgcagttca actacacgct ggaaccggca aaatcgagca tgccaatgac
    52321 gccgatgacc ctgcgtggca tgtatttcta tatggctgat gcggcgacct ttactgattg
    52381 cgcgactgga aaacgtttta tggtggcgaa taacgcagag ctggagcgtg gctacctggc
    52441 tgcgcgcggc aacggcgaaa aaccggtgtt actgtcagta gaaggtcact ttacgcttga
    52501 ggctaatccg gataccggtg cgccgaccaa agtattagcg cccgatacgg caggtaaatt
    52561 ttacccgaac caggattgca gtagtttggg gcagtaaccc gtcttgaggc agaaacaaat
    52621 gaaatattgc ctcatgcgct gcgcttatca ggcctaaagc atacaatgca atattttgaa
    52681 ttagcacgat tttgtaggcc ggataaggcg ttcacgccgc atccggcatt ttgcgcacga
    52741 ggctggcttt tatcactgag tcagtccaac aaagcgactt gctttaatat aaatccctgg
    52801 cttcaggtgc ccaatatact gtaactgcgt ttctggttta aatgcagaga catcaccact
    52861 acgcatatgc atcagatcgg cagggctaat ccagcctgat tgcgccagcg taagcggatg
    52921 cccggctttg gcaaatgcat ccgtgacaaa ttccgaacaa aaccacgact ttttgtctcc
    52981 ttcgccaacg ttacttaact gcgctttcgc caggccgctg acgcactgtt ggcgaaaatc
    53041 ctcggagaac ggattcagtg agcacatctg gcgagtcacc ataaatggaa taaattcgac
    53101 aatgccgcga tagttataac cgctatcttt aattttattg gcgaacgcgg tgatttctgt
    53161 ggcttgttgc ggggtaaggt ccgggactcg taagacgaaa agcttatcac tgtgcttcat
    53221 ggctttttta agggaaacaa tctggacgcc agcgcctgtc gcttcggcaa cgttattatt
    53281 tcccaaatag attgcaacgt gactcacaga ggaagtgctg aatacgcgga taccaaatga
    53341 ggttacccca aggcttgagg agaaaagcaa atcgccgggt ttgagatctg gttccgttat
    53401 ttcttttatt gattgttcgg tgaaagagct ttgatgctgg aatttaacag cccatgtttt
    53461 cgcctcggca tttaccgctg tggctgatga gtctggctgg ctgatatcaa cggtacaggc
    53521 cgaaagtaaa agaaagcagg ggagaagcag gcggcagtac gcctttggtt tatccatttt
    53581 atacaatcca tgtaaaaaag ggccccgaaa ttcaggaccc tttctggcat cagcctttaa
    53641 tctgtttcac cagataatcg acgatgtcac cagtcttaat taactgtttc tcgccgttac
    53701 gacgatattt atattcgata tcatcgttgt cgaggttacg gtcgcccagc acaatagtgt
    53761 gcggaatacc gatcagttcc atatcagcaa acatcacgcc cggacgctct ttgcggtcat
    53821 ccagcagcac ttcgatacct tgtgcgcgca gttcgctgta cagtttctca gcaagttcct
    53881 gtacgcggaa ggatttgtgc atgttcattg gcagaatcgc cacctggaac ggcgcgatag
    53941 cgtcaggcca tacgatgccg cgttcgtcgt agttctgctc aatcgccgct gctaccacac
    54001 gcgttacgcc gataccgtag caacccatcg tcaggatttg gttacggcca tcttcaccct
    54061 gtacggaggc tttcagtgct tcggagtact tggtacccag ctggaagatg tgaccgactt
    54121 cgataccacg tttgatcagc agcgtaccct gaccatccgg gcttggatcg ccagccacca
    54181 cgttgcggat atcagcaatt tccggggtag cgacatcgcg atcccagttg ataccgaagt
    54241 agtgtttacc atcgatgtta gcaccagcag cgaaatcact catcgccgca acggtacggt
    54301 caatcaccac cggaatcggc atgtttaccg gacccagtga acccggaccg gctttaacca
    54361 cggcacgaat ttcttcttcg gtcgcaaaag tcagcgggct ggcaacctgc ggcagttttt
    54421 ctgctttaac ttcgttcagc tcgtggtcac cgcgcaccag cagcgcaacc agcgggaagc
    54481 tactgccttc aacggcttta accagcagag tcttaaccgt tttctcaatc ggcagattga
    54541 actgttcaac cagttccgcg atggttttcg cgttcggcgt atcaaccagc gtcatttcct
    54601 gggtagcagc agcgcgcggt tctttcggcg cgatagcttc tgccagttca atgttcgctg
    54661 cgtagtcaga agtatcggag aagaccacat cgtcttcacc gctctgcgcc agcacctgaa
    54721 attcgtgaga ggcgctaccg ccgatagaac cggtatcggc ctgtacggcg cggaaatcca
    54781 gccccatgcg gctgaagatt ttgctgtagg ccgcatacat tgcatcgtag gtttcctgca
    54841 gggattcctg agaagtatgg aaagagtaag catctttcat caggaattcg cgggaacgca
    54901 tgacgccaaa acgcggacgc acttcgtcgc ggaacttggt ctggatctga tagaagttca
    54961 gcggcagttg tttgtaagag ctcagctcgt tacgaatcag gtcagtgata acttcttcat
    55021 gagttgggcc gagtacgaac ggacgctcgc cacggtcaac aaaacgcagc agttccggac
    55081 cgtactgttc ccaacgacca ctctcttgcc acaaatcggc tggctgaacc accggcatta
    55141 acacctcgat cgcaccggcg ttgttcatct cttcacgcac gatgttttcg acttttttca
    55201 gaacgcgcac gccggtcggc agccaggtat ataacccgga ggccagcttg cggatcatcc
    55261 cggcgcgcag catcagctga tggctgatca cctcggcgtc ggcaggtgtc tccttgagag
    55321 tggagagcag gtattggcta gtacgcatgt tgttacggtt ccagttggaa ggtagaacag
    55381 gctcaaggcg agcctgggac aaaaaaagtg atttagttta ccagtgcaaa agaaatgtca
    55441 aaagagaagg gcgtgaattt aacgcggttc cagcgcaaag acttcaaaac ctgcgtcggt
    55501 gacgcgccag cgaacgttaa aatcatgcaa ccagacggca taggttttgc ccgtttcctc
    55561 acctttacga taggccgggc gcgggtcctg cgccagtact tcgcggataa acagcgttaa
    55621 ctgcggataa cgcttctcca gcgtcaaaag ctgcttttcg acctctgcgg taaaacaaac
    55681 cgccatttct gccgctggcg cactttgcgc atagctggca ctggcatcgg gaagcgattc
    55741 agcaaagggg agatacggtt tgatatccac taccggcgta ccatcgacca gatccagact
    55801 gccgagcttc agaatcacgc tgtctttatg gcaaacaacc tctttcagct ctaccagcga
    55861 cataccaatc gggttggggc gaaaagtgga gcgcgtagcg aaaacaccca ttctggcatt
    55921 accgccgaga cgaggcggac gcacagtcgg acgccagccg ccttccatcg tttgatgaaa
    55981 gacgaaaagg atccataaat ggctgaatgc ttccaggccg cgaacggcgt cggcctggtt
    56041 gtagggagca atgagatgta gttcgccgtt ggcgcttttt accagacctg gctggcgcgg
    56101 aacggcgaat ttttctttat agggcgagcg aataacgcct atttgctcga actggaaact
    56161 gctcatttcg ccgtgatgtt aagcgcagaa ccgatacata cagcctgacg atagcaacct
    56221 ggcgtaccgc tggtgacttc gcagctatgc agtaataccg cattggcttt cattttcgag
    56281 gcgttgattt gcatccgctt acgtgcggtt ggaatgctcg gcggagagtc ctgattagag
    56341 gcctggcaag agtcgccact gacttcaccg agatcgcgga acggtttgcc gactaattct
    56401 tctgcattgg tataaattcg gaccggcgtg gcgcgcggcg ctttcggttt tgcaggctcc
    56461 gctttcggct ggggtgcagt gctttgaacg ggttcgacag gggatctgct taacatggaa
    56521 cagccgctta gcatgagtgc tactaaacag atcggtaaag cacgcatagt atttcctcaa
    56581 tgtatgatca aaacgtcaat attgaatcag gcgcttgtaa aaatgacaag acgggcaagc
    56641 gcccgtcctg aatgatatta caaattgtgg aaacagccta aatattacca gcctttaaca
    56701 gcgccgccgt taaacacttt gtttgctgct tcgtaaactt cgtcagactg ataagcctga
    56761 acgaatttct tcacgttttc ggcgtctttg ttgtcttcac gcgttacgat caggtttacg
    56821 tacggggact ctttatcttc gacaaagata ccgtctttcg ctggagtcag gccaatctgg
    56881 ctggcatagg tggtattgat aactgccaga gcgatttgcg cgtcgtccag agagcgcggc
    56941 agctgcggtg cttccagttc aacaattttc agattttttg ggttctcaac aacatcaaga
    57001 acggtcggca gcaggccaac gccatctttc agtttgatca agcccacttt ttgcagcagc
    57061 agcagtgaac gaccaaggtt agttgggtcg tttggcacgg caacctgcga accatcctgc
    57121 agttcatcca gtgatttgat tttcttggag taaccagcaa tcggataaac aaatgtgttg
    57181 cctactgcga ccagtttgta gccacgatct ttcagttgct gatcaaggta cggtttatgc
    57241 tggaaggcgt tggcgtcgat atcgcctttg ctcaatgctt cgtttggcag aacatagtcg
    57301 ttgaaggtta ccagctcaac gtccaggcca tatttgtctt tcgcaacttt ctgcgcgact
    57361 tctgcaacct gctgttcggc accaacaatc acgccgactt taatgtggtt tggatctttt
    57421 tcatcctgac cgcagcctac cagtgccagt gatccaatca gggctcccac tgccgcaaag
    57481 gttttgaatt tgaacgccat accttattcc ttcttcaatt atttatgttg tgttgaacgt
    57541 tacttgcgag tgacagcccg gacgatgcgg tcgcctgcga actgaattaa ataaaccaga
    57601 atgaccagca ataccagtac cgtattcatc accgtcgcgt tgtagccgat gtagccatac
    57661 tgatagccaa tctgacctaa accaccggca ccgactgcac cacccatcgc ggaataaccg
    57721 accagggtaa tcagggtaat agttgccgca ttcaccagac ccggcaacgc ttccggtaac
    57781 agcactttac ggacgatctg cattggcgtg gcacccattg cgcgggaggc ttcaattaac
    57841 ccggttggga tctccagaag agcgttctcg accatacggg caataaacgg tgctgcacca
    57901 acggttaacg gaacaatcgc tgcctgcaat ccaatcgatg taccgacaat aacgcgggta
    57961 aacggaatca tccataccag caagataatg aacgggatgg aacggaaaat gttcacaatc
    58021 gcagaaatgg tacgatacaa cttcgcgtta gcaataattt gccccggacg cgtgacataa
    58081 agcagaacgc caaccggcag accaatcaca aagccaaaaa aaccggatac gaaggtcatt
    58141 gccagcgttt cccatacgcc acgaaccagc agccacatca tcggctcaga cataacccag
    58201 tacctctact tttacatggt gttcctgcag ccaggcaatg gcggcttgcg tatcttgttg
    58261 tgtgccgtgc atttcagtca gcataatgcc gaacttaacg ccaccggcgt aatccatctg
    58321 cgcgctaata atgttgttgt tgacgttgaa acgacgcgcg gtttcagaaa gcagtggggc
    58381 atcgaccgat tgaccggtaa actccagacg cagcatcggc acgcagtcag taaatggctc
    58441 cgcttgcaga cgttcctggt aatcttccgg gatatccaga tgcagggtcg actgaataaa
    58501 cttctgcgcc agcggcgttt tcggatgcga gaacacttca cttaccgtgt cctgctcgat
    58561 cagttcgcca ttgctgatga ccgccacgca atcacaaatg cgcttcacaa cgtccatttc
    58621 gtgagtgatc aacagaatcg tcaaacccag acggcggttg atgtctttca gcagttcgag
    58681 aatagaacgt gtcgttgccg ggtccagcgc gctggtggct tcatcacaca gcaatacttt
    58741 gggattgctg gctaatgcac gggcaatcgc cacacgttgt ttctgcccac cggaaagatt
    58801 cgacgggtag ctatcatgct tatcgccaag accaaccaat gacagcaatt ccgtcacgcg
    58861 acgtttgatc tcgtctttcg gtgtgttgtc cagctccagc ggcagagcca cgttgccaaa
    58921 aacagtacgc gaagagagca ggttaaaatg ctggaaaatc ataccaatct ggcgacgagc
    58981 tttggtcaac tcggattctg acagcgtggt cagttcctgg ccatcgacca gcacgctacc
    59041 ctcggttggg cgctccagca ggtttacaca acgtataagc gtactcttac ccgcgcctga
    59101 ggcaccgata acgccataaa tttgtccagc tggcacatgc aggctgacgt tgttcaacgc
    59161 ctggatggtg cgggtgccct ggtggaacac tttggtgata ttcgaaagtt ttatcattga
    59221 ttatttatta tcgtcattaa gttagtcgtg gcatttcgaa tgcctggaac gggcaacgcc
    59281 gtcaatgaaa tggatgttaa ggcatccaga cgtctaaatc aatcaggttt atgcgaagag
    59341 cactttcttg caggtcgaaa catgcgatac tagcgtcaca tgccttatta aggagctata
    59401 aaaggtggcg aagagcgtac ccgcaatttt tcttgaccgt gatggcacca ttaatgtcga
    59461 tcacggttat gtccatgaga tcgacaactt tgaatttatc gacggtgtta ttgacgccat
    59521 gcgcgagcta aaaaaaatgg gctttgcgct ggtggtagta accaaccagt ctggcattgc
    59581 tcgcggtaaa tttactgaag cacagtttga aacgctgacc gagtggatgg actggtcgct
    59641 ggcggaccgc gatgtcgatc tggatggtat ctattattgc ccgcatcatc cgcagggtag
    59701 tgttgaagag tttcgccagg tctgcgactg ccgcaaacca catccgggga tgtttttgtc
    59761 agcacgcgat tatttgcata ttgatatggc cgcttcttat atggtgggcg ataaattaga
    59821 agatatgcag gcagcggctg cggcgagcgt gggaacaaaa gtgctggtgc gtacgggtaa
    59881 acctattacg cctgaagcag aaaacgcggc ggattgggtg ttaaatagcc tggcagacct
    59941 gccgcaagcg ataaaaaagc agcaaaaacc ggcgtaatga ttaaaagatg agcggttgaa
    60001 ataaaaatgc atttttccgc ttgtcttcct gagac
//
LOCUS       DAHHTG010000008        59859 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.008, whole genome shotgun sequence.
ACCESSION   DAHHTG010000008 DAHHTG010000000
VERSION     DAHHTG010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 59859)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 59859)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..59859
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.008"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            157..750
                     /locus_tag="NBI74_000542"
     CDS             157..750
                     /locus_tag="NBI74_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001298696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1930918.1"
                     /translation="MTADFGVNFKEAEFYSFYESNVLNHAVAGRNHTVSVMTHVRLFD
                     SDMTFFGKIYGQWDNSWGDDLDMFYGFGYLGWNGEWGFFKPYIGLHNQSGDYVSAKYG
                     QTNGWNGYVVGWTAVLPFTLFDEKFVLSNWNEIELDRNDAYTEQQFGRNGLNGGLTIA
                     WKFYPRWKASVTWRYFDNKLGYDGFGDQMIYMLGYDF"
     gene            771..2264
                     /locus_tag="NBI74_000543"
     CDS             771..2264
                     /locus_tag="NBI74_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HCD1930919.1"
                     /translation="MQKTLMASLIGLAVCTGNAFSPALAAEAKQPNLVIIMADDLGYG
                     DLATYGHQIVKTPNIDRLAQEGVKFTDYYAPAPLSSPSRAGLLTGRMPFRTGIRSWIP
                     SGKDVALGRNELTIANLLKAQGYDTAMMGKLHLNAGSDRTDQPQAQDMGFDYSLVNTA
                     GFVTDATLDNAKERPRYGMVYPTGWLRNGQPTPRADKMSGEYVSSEVVNWLDNKKDSK
                     PFFLYVAFTEVHSPLASPKKYLDMYSQYMSAYQKQHPDLFYGDWADKPWRGVGEYYAN
                     ISYLDAQVGKVLDKIKAVGEEDNTIVIFTSDNGPVTREARKVYELNLAGETDGLRGRK
                     DNLWEGGIRVPAIIKYGKHLPQGMVSDTPVYGLDWMPTLAKMMNFKLPTDRTFDGESL
                     VPVLEQKALKREKPLIFGIDMPFQDDPTDEWAIRDGDWKMIIDRNNKPKYLYNLKSDR
                     YETLNLIGKKPDIEKQMYGKFLKYKTDIDNDSLMKARGDKPEAVTWG"
     gene            2393..3652
                     /locus_tag="NBI74_000544"
     CDS             2393..3652
                     /locus_tag="NBI74_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001356890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytoplasmic protein"
                     /protein_id="HCD1930920.1"
                     /translation="MFTNVNVDCCKTPGCKNLGLLNSQDYVAQGKNILCRECGYLFPV
                     ISEQSLNIYRNIVNHSWRGLICQCSTCGGTSLKKYGYSAQGQRRMYCHHCEKTFITLE
                     HVITTPRGAQLALMIEQGEALADIRKSLLLNSTGLSRELLKLAREANYKESRQCFPAS
                     DITLSTRAFRVKYNGSNNSLYALVTAEEQSGRVVAISTNYSPSAVEQHYQYTSNYEER
                     MSPGTLAHHVQRKELLTMRRDTLFDIDYGPAVLHQNDPGMLVKPVLPAYRHFELVRIL
                     TDEHSNNVQHYLDHECFILGGCLMANLQHIHQGRCHISFVKERGVAPATIDFPPRLFL
                     SGGVRNNVWRAFSNRNYSMAVCNLTGSKKVREMRHATLNSATRFIHFVENHPFLISLN
                     RMSPANVVSTLDILKHLWNKKLEHGTI"
     gene            3887..5053
                     /gene="nhaA"
                     /locus_tag="NBI74_000545"
     CDS             3887..5053
                     /gene="nhaA"
                     /locus_tag="NBI74_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HCD1930921.1"
                     /translation="MKHLHRFFSSDASGGIILIIAAVLAMIMANSGATSGWYHDFLET
                     PVQLRVGTLEINKNMLLWINDALMAVFFLLVGLEVKRELMQGSLASLRQAAFPVIAAI
                     GGMIVPALLYLAFNYADPITREGWAIPAATDIAFALGVLALLGSRVPLALKIFLMALA
                     IIDDLGAIIIIALFYTNDLSMASLGVAAVAIAVLAVLNLCGVRRTGVYILVGVVLWTA
                     VLKSGVHATLAGVIVGFFIPLKEKHGRSPAKRLEHVLHPWVAYLILPLFAFANAGVSL
                     QGVTLEGLTSILPLGIIAGLLIGKPLGISLFCWLALRLKLAHLPEGTTYQQIMAVGIL
                     CGIGFTMSIFIASLAFGSVDPELINWAKLGILVGSISSAVIGYSWLRVRLRPSV"
     gene            5119..6018
                     /gene="nhaR"
                     /locus_tag="NBI74_000546"
     CDS             5119..6018
                     /gene="nhaR"
                     /locus_tag="NBI74_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional activator NhaR"
                     /protein_id="HCD1930922.1"
                     /translation="MSHINYNHLYYFWHVYKEGSVVGAAEALYLTPQTITGQIRALEE
                     RLQGKLFKRKGRGLEPSELGELVYRYADKMFTLSQEMLDIVNYRKESNLLFDVGVADA
                     LSKRLVSSVLNAAVVEGEPIHLRCFESTHEMLLEQLSQHKLDMIISDCPIDSTQQEGL
                     FSVRIGECGVSFWCTNPPPEKPFPACLEERRLLIPGRRSMLGRKLLNWFNSQGLNVEI
                     LGEFDDAALMKAFGAMHNAIFVAPTLYAYDFYADKTVVEIGRVENVMEEYHAIFAERM
                     IQHPAVQRICNTDYSSLFSPASR"
     gene            complement(6076..8115)
                     /locus_tag="NBI74_000547"
     CDS             complement(6076..8115)
                     /locus_tag="NBI74_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 31 protein"
                     /protein_id="HCD1930923.1"
                     /translation="MSFIKQDPHRLVWQQDDRYLWIEAWGENSLRVRSGRHLPMMRNE
                     NWALTEEKGDAVAYITYEEKQATLQNGKITAIVNLQGQISFWRNAEKCLLQEFWRQRG
                     EIGEDESAHGQYVSALNLQAREFKPIPGGKYTIKARFEANDGEKLFGMGQYQQPNLDL
                     KGCMLELAQRNSQASVPFLLSNRGYGFLWNNPAVGRVTFAQNGTEWVAEVSEQLDYWV
                     TAGDTPAEISAAYARVTGTPPMMPDYAMGFWQCKLRYRNQQELLEVARGYKQRNLPIS
                     VIVIDFFHWPNQGDWMFDLRDWPDPDAMIAELKEMGIELMVSFWPTVDNRTESYREMK
                     ENGWLVHTERGLPINMDFLGNTTFFDATHPGAREYVWNKAKRNYYDKGVKLFWLDEAE
                     PEFGVYDYDNYRYYAGPVLEVGNIYPRMYAKTFFDGMTAAGEKQVINLLRCAWAGSQK
                     YGALVWSGDIHSSFRSLRNQFAAGLNMGIAGIPWWTTDIGGFHGGNIHDPKFHELLIR
                     WFQWGVFSPVMRLHGNRDPQVLPEQPYRDGIAQCPTGAPNEVWSYGEETGEILTSCLQ
                     LREKLKSYISKIMAETHEKNSPIMRTMFFEFPDQAESWNIYDQYCFGPDLLVAPVMHE
                     GTRSRDVWLPAGETWIDFYTHEHYAGGQTLHHCAPLHQIPVFIREKGKYRQIFPV"
     gene            complement(8143..9516)
                     /locus_tag="NBI74_000548"
     CDS             complement(8143..9516)
                     /locus_tag="NBI74_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000100025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCD1930924.1"
                     /translation="MSSVLETDKSSASDSLPLIQRISYGSLDVAGNLLYCFGSTYILY
                     FYTDVAGISLAVAGIILLLARIVDGIDAPVWGIIIDKTHSRYGKCRPWFLWLPLPFAV
                     FSALSFWSPDISMTGKTIYSAISYMLASILFTGLNTPLSAILPLMTLSPKERLVLNSY
                     RMTGGQIGVLLMNATALPLVAFLGNGNDKAGFIYTAIVFSVISCALTLFAFKNIRELD
                     TDKIQQEPRLPMKKSFSAMKGNWPWILMVLANLIFWIALQQRNTTIVYYLTYNLDRKD
                     LVPLINSLATIQILFIIAIPFFSRYLSKTWIWITGLLVAMFGGGLMWLAGDNIPLMIA
                     AWMLANIGSGIACSMPFAMLGFAVDFGRWKTGIKATGILIAFGSTFCIKMGSGIGTAF
                     AAWIMNSFGYIPNQPQTAAGLDGISWAFIWVPAVLFALAAIPLLFFRKYEAMEECIQH
                     DLQVHNP"
     gene            complement(9905..10168)
                     /gene="rpsT"
                     /locus_tag="NBI74_000549"
     CDS             complement(9905..10168)
                     /gene="rpsT"
                     /locus_tag="NBI74_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006117633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HCD1930925.1"
                     /translation="MANIKSAKKRAIQSEKARKHNASRRSMMRTFIKKVYAAIEAGDK
                     AAAQKAFNEMQPIVDRQAAKGLIHKNKAARHKANLTAQINKLA"
     gene            10271..10489
                     /locus_tag="NBI74_000550"
     CDS             10271..10489
                     /locus_tag="NBI74_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2575 family protein"
                     /protein_id="HCD1930926.1"
                     /translation="MCRHSLRSDGAGFYQLAGCEYSFSARKIAVGGQFLPMICAIAMK
                     SHFFLISVLNRRLTLTAVQGILGRFSLF"
     gene            10497..11438
                     /gene="ribF"
                     /locus_tag="NBI74_000551"
     CDS             10497..11438
                     /gene="ribF"
                     /locus_tag="NBI74_000551"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HCD1930927.1"
                     /translation="MKLIRGIHNLSQAPQEGCVLTIGNFDGVHRGHRALLQGLQEEGR
                     KRNLPVMVMLFEPQPLELFATDKAPARLTRLREKLRYLAECGVDYVLCVRFDRRFAAL
                     TAQNFISDLLVKHLRVKFLAVGDDFRFGAGREGDFLLLQKAGMEYGFDITSTQTFCEG
                     GVRISSTAVRQALADDNLALAESLLGHPFAISGRVVHGDELGRTIGFPTANVPLRRQV
                     SPVKGVYAVEVLGLGEKPLPGVANIGTRPTVAGIRQQLEVHLLDVAMDLYGRHIQVVL
                     RKKIRNEQRFASLDELKAQIARDELTAREFFGLTKPA"
     gene            11481..14297
                     /gene="ileS"
                     /locus_tag="NBI74_000552"
     CDS             11481..14297
                     /gene="ileS"
                     /locus_tag="NBI74_000552"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HCD1930928.1"
                     /translation="MSDYKSTLNLPETGFPMRGDLAKREPGMLARWTDDDLYGIIRAA
                     KKGKKTFILHDGPPYANGSIHIGHSVNKILKDIIIKSKGLSGYDSPYVPGWDCHGLPI
                     ELKVEQEYGKPGEKFTAAEFRAKCREYAATQVDGQRKDFIRLGVLGDWSHPYLTMDFK
                     TEANIIRALGKIIGNGHLHKGAKPVHWCVDCRSALAEAEVEYYDKTSPSIDVAFQAVD
                     QDALKTKFGVSNVNGPISLVIWTTTPWTLPANRAISIAPDFDYALVQIDGQAVILAKD
                     LVESVMQRIGVSDYTILGTVKGAELELLRFTHPFMDFDVPAILGDHVTLDAGTGAVHT
                     APGHGPDDYVIGQKYGLETANPVGPDGTYLPGTYPTLDGVNVFKANDIVIALLQEKGA
                     LLHVEKMQHSYPCCWRHKTPIIFRATPQWFVSMDQKGLRAQSLKEIKGVQWIPDWGQA
                     RIESMVANRPDWCISRQRTWGVPMSLFVHKDTEELHPRTLELMEEVAKRVEVDGIQAW
                     WDLDAKEILGDEADQYVKVPDTLDVWFDSGSTHSSVVDVRPEFAGHAADMYLEGSDQH
                     RGWFMSSLMISTAMKGKAPYRQVLTHGFTVDGQGRKMSKSIGNTVSPQDVMNKLGADI
                     LRLWVASTDYTGEMAVSDEILKRAADSYRRIRNTARFLLANLNGFDPAKDMVKPEEMV
                     VLDRWAVGCAKAAQEDILKAYEAYDFHEVVQRLMRFCSVEMGSFYLDIIKDRQYTAKA
                     DSVARRSCQTALYHIAEALVRWMAPILSFTADEVWGYLPGEREKYVFTGEWYEGLFGL
                     ADSEAMNDAFWDELLKVRGEVNKVIEQARADKKVGGSLEAAVTLYAEPELAAKLTALG
                     DELRFVLLTSGATVADYNDAPADAQQSEVLKGLKVALSKAEGEKCPRCWHYTQDVGKV
                     AEHAEICGRCVSNVAGDGEKRKFA"
     gene            14297..14791
                     /gene="lspA"
                     /locus_tag="NBI74_000553"
     CDS             14297..14791
                     /gene="lspA"
                     /locus_tag="NBI74_000553"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HCD1930929.1"
                     /translation="MSQSICSTGLRWLWLVVVVLIIDLGSKYLILQNFALGDTVPLFP
                     SLNLHYARNYGAAFSFLADSGGWQRWFFAGIAIGISVILVVMMYRSKAAQKLNNIAYA
                     LIIGGALGNLFDRLWHGFVVDMIDFYVGDWHFATFNLADTAICVGAALIVLEGFLPSK
                     AKKQ"
     gene            14899..15348
                     /gene="fkpB"
                     /locus_tag="NBI74_000554"
     CDS             14899..15348
                     /gene="fkpB"
                     /locus_tag="NBI74_000554"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HCD1930930.1"
                     /translation="MSESVQSNSAVLVHFTLKLDDGTTAESTRNNGKPALFRLGDASL
                     SEGLEQHLLGLKVGDKTTFSLEPDAAFGVPSPDLIQYFSRREFMDAGEPEIGAIMLFT
                     AMDGSEMPGVIREINGDSITVDFNHPLAGQTVHFDIEVLEIDPALEA"
     gene            15350..16300
                     /gene="ispH"
                     /locus_tag="NBI74_000555"
                     /gene_synonym="lytB"
     CDS             15350..16300
                     /gene="ispH"
                     /locus_tag="NBI74_000555"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HCD1930931.1"
                     /translation="MQILLANPRGFCAGVDRAISIVENALAIYGAPIYVRHEVVHNRY
                     VVDSLRERGAIFIEQISEVPDGAILIFSAHGVSQAVRNEAKSRDLTVFDATCPLVTKV
                     HMEVARASRRGEESILIGHAGHPEVEGTMGQYSNPEGGMYLVESPDDVWKLTVKNEEK
                     LSFMTQTTLSVDDTSDVIDALRKRFPKIVGPRKDDICYATTNRQEAVRALAEQAEVVL
                     VVGSKNSSNSNRLAELAQRMGKRAFLIDDAKDIQEEWVKEVKCVGVTAGASAPDILVQ
                     NVVARLQQLGGGEAIPLEGREENIVFEVPKELRVDIREVD"
     gene            16366..17280
                     /gene="rihC"
                     /locus_tag="NBI74_000556"
     CDS             16366..17280
                     /gene="rihC"
                     /locus_tag="NBI74_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001239167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside hydrolase RihC"
                     /protein_id="HCD1930932.1"
                     /translation="MHLPIFLDTDPGIDDAVAIAAAIFTPELDLQLMTTVAGNVSVEK
                     TTRNALQLLHFWNVDIPLAQGAAVPLVRAPRDAASVHGESGMAGYNFVEHNRQPLGIP
                     AFLAIRDALMRAPEPVTLVAIGPLTNIALLLSQCPECKPYIRRLVIMGGSAGRGNCTP
                     NAEFNIAADPEAAACVFRSGIEIVMCGLDVTNQAILTPDYLATLPELNRTGKMLHALF
                     SHYRSGSMQSGLRMHDLCAIAWLVRPELFTLKPCFVAVETQGEFTSGTTVVDIDGCLG
                     KPANVQVALDLDVKGFQQWVAEVLALAL"
     gene            17303..17668
                     /locus_tag="NBI74_000557"
     CDS             17303..17668
                     /locus_tag="NBI74_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000220878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HCD1930933.1"
                     /translation="MTYGEAYLEGWKNIFNYEGVSNRFEFWSFMIGSGVICLLPLLCW
                     WLAVTINNDYGVFIFFALPASFILTLIFAVPAIALAVRRMHDIGYSGWWVTIVVLIPV
                     TGVILLILCCLPSKSQDQA"
     gene            17829..18650
                     /gene="dapB"
                     /locus_tag="NBI74_000558"
     CDS             17829..18650
                     /gene="dapB"
                     /locus_tag="NBI74_000558"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HCD1930934.1"
                     /translation="MHDANIRVAIAGAGGRMGRQLIQAALALEGVQLGAALEREGSSL
                     LGSDAGELAGAGKTGVTVQSSLDAIKDDFDVFIDFTRPEGTLNHLAFCRQHGKGMVIG
                     TTGFDEAGKQAIRDAAADIAIVFAANFSVGVNVMLKLLEKAAKVMGDYTDIEIIEAHH
                     RHKVDAPSGTALAMGEAIAHALDKDLKDCAVYSREGHTGERVPGTIGFATVRAGDIVG
                     EHTAMFADIGERLEITHKASSRMTFANGAVRSALWLSGKESGLFDMRDVLDLNNL"
     gene            19106..20254
                     /gene="carA"
                     /locus_tag="NBI74_000559"
     CDS             19106..20254
                     /gene="carA"
                     /locus_tag="NBI74_000559"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HCD1930935.1"
                     /translation="MIKSALLVLEDGTQFHGRAIGATGSAVGEVVFNTSMTGYQEILT
                     DPSYSRQIVTLTYPHIGNVGTNDADEESSQVHAQGLVIRDLPLIASNFRNTEDLSSYL
                     KRHNIVAIADIDTRKLTRLLREKGAQNGCIIAGDNPDAALALEKARAFPGLNGMDLAK
                     EVTTAEPYSWTQGSWTLTGGLPEAKKEDELPYHVVAYDFGAKRNILRMLVDRGCRLTI
                     VPAQTSAEDVLKMNPDGIFLSNGPGDPAPCDYAITAIQKFLETDIPVFGICLGHQLLA
                     LASGAKTVKMKFGHHGGNHPVKDVEKNVVMITAQNHGFAVDEATLPANLRVTHKSLFD
                     GTLQGIHRTDKPAFSFQGHPEASPGPHDAAPLFDHFIALIEQYRKTAK"
     gene            20272..23493
                     /gene="carB"
                     /locus_tag="NBI74_000560"
     CDS             20272..23493
                     /gene="carB"
                     /locus_tag="NBI74_000560"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HCD1930936.1"
                     /translation="MPKRTDIKSILILGAGPIVIGQACEFDYSGAQACKALREEGYRV
                     ILVNSNPATIMTDPEMADATYIEPIHWEVVRKIIEKERPDAVLPTMGGQTALNCALEL
                     ERQGVLEEFGVTMIGATADAIDKAEDRRRFDVAMKKIGLETARSGIAHSMEEALAVAA
                     EVGFPCIIRPSFTMGGSGGGIAYNREEFEEICARGLDLSPTKELLIDESLIGWKEYEM
                     EVVRDKNDNCIIVCSIENFDAMGIHTGDSITVAPAQTLTDKEYQIMRNASMAVLREIG
                     VETGGSNVQFAVNPKNGRLIVIEMNPRVSRSSALASKATGFPIAKVAAKLAVGYTLDE
                     LMNDITGGRTPASFEPSIDYVVTKIPRFNFEKFAGANDRLTTQMKSVGEVMAIGRTQQ
                     ESLQKALRGLEVGATGFDPKVSLDDPEALTKIRRELKDAGAERIWYIADAFRAGLSVD
                     GVFNLTNIDRWFLVQIEELVRLEEKVAEVGITGLNAEFLRQLKRKGFADARLAKLAGV
                     REAEIRKLRDQYDLHPVYKRVDTCAAEFATDTAYMYSTYEEECEANPSTDREKIMVLG
                     GGPNRIGQGIEFDYCCVHASLALREDGYETIMVNCNPETVSTDYDTSDRLYFEPVTLE
                     DVLEIVRIEKPKGVIVQYGGQTPLKLARALEAAGVPVIGTSPDAIDRAEDRERFQHAV
                     DRLKLKQPANATVTTIEMAVEKAKEIGYPLVVRPSYVLGGRAMEIVYDEADLRRYFQT
                     AVSVSNDAPVLLDHFLDDAVEVDVDAICDGEMVLIGGIMEHIEQAGVHSGDSACSLPA
                     YTLSQEIQDVMRQQVQKLAFELQVRGLMNVQFAVKNNEVYLIEVNPRAARTVPFVSKA
                     TGVPLAKVAARVMAGKSLAEQGVTKEVIPPYYSVKEVVLPFNKFPGVDPLLGPEMRST
                     GEVMGVGRTFAEAFAKAQLGSNSTMKKHGRALLSVREGDKERVVDLAAKLLKQGFELD
                     ATHGTAIVLGEAGINPRLVNKVHEGRPHIQDRIKNGEYTYIINTTSGRRAIEDSRVIR
                     RSALQYKVHYDTTLNGGFATAMALNADATEKVISVQEMHAQIK"
     gene            23754..24149
                     /gene="caiF"
                     /locus_tag="NBI74_000561"
     CDS             23754..24149
                     /gene="caiF"
                     /locus_tag="NBI74_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414576.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine metabolism transcriptional regulator
                     CaiF"
                     /protein_id="HCD1930937.1"
                     /translation="MCEGYVEKPLYLLIAEWMMAENRWVIAREISIHFDIEHSKAVNT
                     LTYILSEVTEISCEVKMIPNKLEGRGCQCQRLVKVVDIDEQIYARLRNNSRDKLVGVR
                     KTPRIPAVPLTELNREQKWQMMLSKSMRR"
     gene            complement(24268..24858)
                     /gene="caiE"
                     /locus_tag="NBI74_000562"
     CDS             complement(24268..24858)
                     /gene="caiE"
                     /locus_tag="NBI74_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine operon protein CaiE"
                     /protein_id="HCD1930938.1"
                     /translation="MSYYAFEGLIPVVHPTAFVHPSAVLIGDVIVGAGVYIGPLASLR
                     GDYGRLIVQAGANIQDGCIMHGYCDTDTIVGENGHIGHGAILHGCVIGRDALVGMNSV
                     IMDGAVIGEESIVAAMSFVKAGFRGEKRQLLMGTPARAVRSVSDDELHWKRLNTKEYQ
                     DLVGRCHASLHETQPLRQMEENRPRLQGTTDVTPKR"
     gene            complement(24864..25649)
                     /gene="caiD"
                     /locus_tag="NBI74_000563"
     CDS             complement(24864..25649)
                     /gene="caiD"
                     /locus_tag="NBI74_000563"
                     /EC_number="4.2.1.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA hydratase"
                     /protein_id="HCD1930939.1"
                     /translation="MSESLHLTRNGSILEITLDRPKANAIDAKTSFEMGEVFLNFRDD
                     PQLRVAIITGAGEKFFSAGWDLKAAAEGEAPDADFGPGGFAGLTEIFNLDKPVIAAVN
                     GYAFGGGFELALAADFIVCADNASFALPEAKLGIVPDSGGVLRLPKILPPAIVNEMVM
                     TGRRMGAEEALRWGVVNRVVSQAELMDNARELAQQLVNSAPLAIAALKEIYRTTSEMP
                     VEEAYRYIRSGVLKHYPSVLHSEDAIEGPLAFAEKRDPVWKGR"
     gene            complement(25758..27311)
                     /gene="caiC"
                     /locus_tag="NBI74_000564"
     CDS             complement(25758..27311)
                     /gene="caiC"
                     /locus_tag="NBI74_000564"
                     /EC_number="6.2.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetaine/carnitine-CoA ligase"
                     /protein_id="HCD1930940.1"
                     /translation="MDIIGGQHLRQMWDDLADVYGHKTALICESSSGVVNRYSYLELN
                     QEINRTANLFYTLGIRKGDKVALHLDNCPEFIFCWFGLAKIGAIMVPINARLLREEST
                     WILQNSQACLLVTSAQFYPMYQQIQQEDASQLRHICLTDMALPADDGVSSFTQLKNQQ
                     PATLCYAPPLSTDDTAEILFTSGTTSRPKGVVITHYNLRFAGYYSAWQCALRDDDVYL
                     TVMPAFHIDCQCTAAMAAFSAGATFVLVEKYSARAFWGQVQKYRATITECIPMMIRTL
                     MVQPPSANDRQHRLREVMFYLNLSEQEKDAFCERFGVRLLTSYGMTETIVGIIGDRPG
                     DKRRWPSIGRAGFCYEAEIRDDHNRPLPAGEIGEICIKGVPGKTIFKEYFLNPKATAK
                     VLEADGWLHTGDTGYRDEEGFFYFVDRRCNMIKRGGENVSCVELENIIATHPKIQDIV
                     VVGIKDSIRDEAIKAFVVLNEGETLSEEEFFRFCEQNMAKFKVPSYLEIRKDLPRNCS
                     GKIIRKNLK"
     gene            complement(27384..28601)
                     /gene="caiB"
                     /locus_tag="NBI74_000565"
     CDS             complement(27384..28601)
                     /gene="caiB"
                     /locus_tag="NBI74_000565"
                     /EC_number="2.8.3.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine CoA-transferase"
                     /protein_id="HCD1930941.1"
                     /translation="MDHLTMPKFGPLSGLRVVFSGIEIAGPFAGQMFAEWGAEVIWIE
                     NVAWADTIRVQPNYPQLSRRNLHALSLNIFKDEGREAFLKLMETTDIFIEASKGPAFA
                     RRGITDEVLWQHNPKLVIAHLSGFGQYGTEEYTNLPAYNTIAQAFSGYLIQNGDVDQP
                     MPAFPYTADYFSGLTATTAALAALHKVRETGKGESIDIAMYEVMLRMGQYFMMDYFNG
                     GEMCPRMTKGKDPYYAGCGLYKCADGYIVMELVGITQIAECFKDIGLAHLLGTPEIPA
                     GTQLIHRIECPYGPLVEEKLDAWLAAHTIAEVKERFAELNIACAKVLTVPELESNPQY
                     VARESITQWQTMDGRTCKGPNIMPKFKNNPGQIWRGMPSHGMDTAAILKNIGYSENDI
                     QELVSKGLAKVED"
     gene            complement(28719..29861)
                     /gene="caiA"
                     /locus_tag="NBI74_000566"
     CDS             complement(28719..29861)
                     /gene="caiA"
                     /locus_tag="NBI74_000566"
                     /EC_number="1.3.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA dehydrogenase"
                     /protein_id="HCD1930942.1"
                     /translation="MDFNLNDEQELFVAGIRELMASENWEAYFAECDRDSVYPERFVK
                     ALADMGIDSLLIPEEHGGLDAGFVTLAAVWMELGRLGAPTYVLYQLPGGFNTFLREGT
                     QEQIDKIMAFRGTGKQMWNSAITEPGAGSDVGSLKTTYTRRNGKIYLNGSKCFITSSA
                     YTPYIVVMARDGASPDKPVYTEWFVDMSKPGIKVTKLEKLGLRMDSCCEITFDDVELD
                     EKDMFGREGNGFNRVKEEFDHERFLVALTNYGTAMCAFEDAARYANQRVQFGEAIGRF
                     QLIQEKFAHMAIKLNSMKNMLYEAAWKADNGTITSGDAAMCKYFCANAAFEVVDSAMQ
                     VLGGVGIAGNHRISRFWRDLRVDRVSGGSDEMQILTLGRAVLKQYR"
     gene            complement(29893..31407)
                     /gene="caiT"
                     /locus_tag="NBI74_000567"
     CDS             complement(29893..31407)
                     /gene="caiT"
                     /locus_tag="NBI74_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine/gamma-butyrobetaine antiport BCCT
                     transporter"
                     /protein_id="HCD1930943.1"
                     /translation="MKNEKRKTGIEPKVFFPPLIIVGILCWLTVRDLDAANVVINAVF
                     SYVTNVWGWAFEWYMVVMLFGWFWLVFGPYAKKRLGNEPPEFSTASWIFMMFASCTSA
                     AVLFWGSIEIYYYISTPPFGLEPNSTGAKELGLAYSLFHWGPLPWATYSFLSVAFAYF
                     FFVRKMEVIRPSSTLVPLVGEKHAKGLFGTIVDNFYLVALIFAMGTSLGLATPLVTEC
                     MQWLFGIPHTLQLDAIIITCWIILNAICVACGLQKGVRIASDVRSYLSFLMLGWVFIV
                     SGASFIMNYFTDSVGMLLMYLPRMLFYTDPIAKGGFPQGWTVFYWAWWVIYAIQMSIF
                     LARISRGRTVRELCFGMVLGLTASTWILWTVLGSNTLLLIDKNIINIPNLIEQYGVAR
                     AIIETWAALPLSTATMWGFFILCFIATVTLVNACSYTLAMSTCREVRDGEEPPLLVRI
                     GWSVLVGIIGIVLLALGGLKPIQTAIIAGGCPLFFVNIMVTLSFIKDAKQNWKD"
     gene            31880..32650
                     /locus_tag="NBI74_000568"
     CDS             31880..32650
                     /locus_tag="NBI74_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein FixA"
                     /protein_id="HCD1930944.1"
                     /translation="MKIITCYKCVPDEQDIAVNNADGSLDFSKADAKISQYDLNAIEA
                     ACQLKQQAAEAQVTALSVGGKALTNAKGRKDVLSRGPDELIVVIDDQFEQALPQQTAS
                     VLAAAAQKAGFDLILCGDGSSDLYAQQVGLLVGEILNIPAVNGVSKIISLTADTLTVE
                     RELEDETETLSIPLPAVVAVSTDINSPQIPSMKAILGAAKKPVQVWSAADIGFNAEAA
                     WSEQQVAAPKQRERQRIVIEGDGEEQIAAFAENLRKVI"
     gene            32665..33606
                     /locus_tag="NBI74_000569"
     CDS             32665..33606
                     /locus_tag="NBI74_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HCD1930945.1"
                     /translation="MNTFSQVWVFSDTPSRLPELMNGAQALANQINTFVLNDADGAQA
                     IQLGANHVWKLSGKPNDRMIEDYAGVMADTIRQHGADGLVLLPNTRRGKLLAAKLGYR
                     LNAAVSNDASTVSVQDGKATVKHMVYGGLAIGEERIATPYAVLTISSGTFDAAQPDAS
                     RTGETHTVEWQDPAVAITRTATQARQSNSVDLDKARLVVSVGRGIGSKENIALAEQLC
                     KAIGAELACSRPVAENEKWMEHERYVGISNLMLKPELYLAVGISGQIQHMVGANASQT
                     IFAINKDKNAPIFQYADYGIVGDAVKILPALTAALAR"
     gene            33657..34943
                     /locus_tag="NBI74_000570"
     CDS             33657..34943
                     /locus_tag="NBI74_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HCD1930946.1"
                     /translation="MSEDIFDAIIVGAGLAGSVAALVLAREGAQVLVIERGNSAGAKN
                     VTGGRLYAHSLEHIIPGFADSAPVERLITHEKLAFMTEKSAMTMDYCNGDETSPSQRS
                     YSVLRSKFDAWLMEQAEEAGAQLITGIRVDNLVQRDGKVVGVEADGDVIEAKTVILAD
                     GVNSILAEKLGMAKRVKPTDVAVGVKELIELPKSVIEDRFQLQGNQGAACLFAGSPTD
                     GLMGGGFLYTNENTLSLGLVCGLHHLHDAKKSVPQMLEDFKQHPAVAPLIAGGKLVEY
                     SAHVVPEAGINMLPELVGDGVLIAGDAAGMCMNLGFTIRGMDLAIAAGEAAAKTVLSA
                     MKSDDFSKQKLAEYRQHLESGPLRDMRMYQKLPAFLDNPRMFSGYPELAVGVARDLFT
                     IDGSAPELMRKKILRHGKKVGFINLIKDGMKGVTVL"
     gene            34940..35227
                     /gene="fixX"
                     /locus_tag="NBI74_000571"
     CDS             34940..35227
                     /gene="fixX"
                     /locus_tag="NBI74_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein FixX"
                     /protein_id="HCD1930947.1"
                     /translation="MTSPVNVDVKLGVNKFNVDEEHPHIVVKADADKQALELLVKACP
                     AGLYKKQDDGSVRFDYAGCLECGTCRILGLGSALEQWEYPRGTFGVEFRYG"
     gene            35286..36617
                     /locus_tag="NBI74_000572"
     CDS             35286..36617
                     /locus_tag="NBI74_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCD1930948.1"
                     /translation="MQPSRNFDDLKFSSIHRRILLWGSGGPFLDGYVLVMIGVALEQL
                     TPALKLDADWIGLLGAGTLAGLFVGTSLFGYISDKVGRRKMFLIDIIAIGVISVATMF
                     VSSPVELLVMRVLIGIVIGADYPIATSMITEFSSTRQRAFSISFIAAMWYVGATCADL
                     VGYWLYDVEGGWRWMLGSAAIPCLLILIGRFELPESPRWLLRKGRVKECEEMMIKLFG
                     EPVAFEEEQPQQTRFRDLFNRRHFPFVLFVAAIWTCQVIPMFAIYTFGPQIVGLLGLG
                     VGKNAALGNVVISLFFMLGCIPPMLWLNTAGRRPLLIGSFAMMTLALAVLGLIPDMGI
                     WLVVMAFAVYAFFSGGPGNLQWLYPNELFPTDIRASAVGVIMSLSRIGTIVSTWALPI
                     FINNYGISNTMLMGAGISLFGLLISVAFAPETRGMSLAQTSNMTIRGQRMG"
     gene            36725..37255
                     /gene="kefF"
                     /locus_tag="NBI74_000573"
     CDS             36725..37255
                     /gene="kefF"
                     /locus_tag="NBI74_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     oxidoreductase KefF"
                     /protein_id="HCD1930949.1"
                     /translation="MILIIYAHPYPHHSHANKRMLEQARTLEGVEIRSLYQLYPDFNI
                     DIAAEQEALSRADLIVWQHPMQWYSIPPLLKLWIDKVFSHGWAYGHGGTALHGKHLLW
                     AVTTGGGESHFEIGAHPGFDVLSQPLQATAIYCGLNWLPPFAMHCTFICDDETLEGQA
                     RHYKQRLLEWQEAHHG"
     gene            37248..39110
                     /gene="kefC"
                     /locus_tag="NBI74_000574"
     CDS             37248..39110
                     /gene="kefC"
                     /locus_tag="NBI74_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefC"
                     /protein_id="HCD1930950.1"
                     /translation="MDSHTLIQALIYLGSAALIVPIAVRLGLGSVLGYLIAGCIIGPW
                     GLRLVTDAESILHFAEIGVVLMLFIIGLELDPQRLWKLRAAVFGGGALQMVICGGLLG
                     LFCMLLGLRWQVAELIGMTLALSSTAIAMQAMNERNLMVTQMGRSAFAVLLFQDIAAI
                     PLVAMIPLLAASSASTTMGAFVLSALKVAGALVLVVLLGRYVTRPALRFVARSGLREV
                     FSAVALFLVFGFGLLLEEVGLSMAMGAFLAGVLLASSEYRHALESDIEPFKGLLLGLF
                     FIGVGMSIDFGTLLENPLRIVILLLGFLIIKIAMLWLIARPLQVPNKQRRWFAVLLGQ
                     GSEFAFVVFGAAQMANVLEPEWAKSLTLAVALSMAATPILLVILNRLEQSSTEEAREA
                     DEIDEEQPRVIIAGFGRFGQITGRLLLSSGVKMVVLDHDPDHIETLRKFGMKVFYGDA
                     TRMDLLESAGAAKAEVLINAIDDPQTNLQLTEMVKEHFPHLQIIARARDVDHYIRLRQ
                     AGVEKPERETFEGALKTGRLALESLGLGPYEARERADVFRRFNIQMVEEMAMVENDTK
                     ARAAVYKRTSAMLSEIITEDREHLSLIQRHGWQGTEEGKHTGNMADEPETKPSS"
     gene            39302..39781
                     /gene="folA"
                     /locus_tag="NBI74_000575"
     CDS             39302..39781
                     /gene="folA"
                     /locus_tag="NBI74_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 dihydrofolate reductase"
                     /protein_id="HCD1930951.1"
                     /translation="MISLIAALAVDRVIGMENAMPWNLPADLAWFKRNTLNKPVIMGR
                     HTWESIGRPLPGRKNIILSSQPGTDDRVTWVKSVDEAIAACGDVPEIMVIGGGRVYEQ
                     FLPKAQKLYLTHIDAEVEGDTHFPDYEPDDWESVFSEFHDADAQNSHSYCFEILERR"
     gene            39867..40100
                     /locus_tag="NBI74_000576"
     CDS             39867..40100
                     /locus_tag="NBI74_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system CcdA family
                     antitoxin"
                     /protein_id="HCD1930952.1"
                     /translation="MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLTVALNAELKKH
                     AATRWREENAEAIAALNQLADETGCFSDEYRSF"
     gene            40103..40417
                     /locus_tag="NBI74_000577"
     CDS             40103..40417
                     /locus_tag="NBI74_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CcdB family protein"
                     /protein_id="HCD1930953.1"
                     /translation="MQFTVYRSRGRNAAFPFVIDVTSDIIGEINRRIVIPLTPIERFS
                     RIRPPERLNPILLLVDGKEYVLMTHETATVPVNALGTKFCDASAHRTLIKGALDFMLD
                     GI"
     gene            complement(40414..41262)
                     /gene="apaH"
                     /locus_tag="NBI74_000578"
     CDS             complement(40414..41262)
                     /gene="apaH"
                     /locus_tag="NBI74_000578"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     ApaH"
                     /protein_id="HCD1930954.1"
                     /translation="MATYLIGDVHGCYDELIALLHKVEFTPGKDTLWLTGDLVARGPG
                     SLDVLRYVKSLGDSVRLVLGNHDLHLLAVFAGISRNKPKDRLTPLLEAPDADELLNWL
                     RRQPLLQIDEEKKLVMAHAGITPQWDLQTAKECARDVEAVLSSDSYPFFLDAMYGDMP
                     NNWSPELRGLGRLRFITNAFTRMRFCFPNGQLDMYSKESPEEAPAPLKPWFAIPGPVA
                     EEYNIAFGHWASLEGKGTPEGIYALDTGCCWGGTLTCLRWEDKQYFVQPSNRHKDLSE
                     GEAVAS"
     gene            complement(41269..41646)
                     /gene="apaG"
                     /locus_tag="NBI74_000579"
     CDS             complement(41269..41646)
                     /gene="apaG"
                     /locus_tag="NBI74_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HCD1930955.1"
                     /translation="MINSPRVCIQVQSVYIEAQSSPDNERYVFAYTVTIRNLGRAPVQ
                     LLGRYWLITNGNGRETEVQGEGVVGVQPLIAPGEEYQYTSGAIIETPLGTMQGHYEMI
                     DENGVPFSIDIPVFRLAVPTLIH"
     gene            complement(41649..42470)
                     /gene="rsmA"
                     /locus_tag="NBI74_000580"
     CDS             complement(41649..42470)
                     /gene="rsmA"
                     /locus_tag="NBI74_000580"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HCD1930956.1"
                     /translation="MNNRVHQGHLARKRFGQNFLNDQFVIDSIVSAINPQKGQAMVEI
                     GPGLAALTEPVGERLDQLTVIELDRDLAARLQTHPFLGPKLTIYQQDAMTFNFGELAA
                     KMGQPLRVFGNLPYNISTPLMFHLFSYTDAIADMHFMLQKEVVNRLVAGPNSKAYGRL
                     SVMAQYYCNVIPVLEVPPSAFTPPPKVDSAVVRLVPHATMPHPVKDVRVLSRITTEAF
                     NQRRKTIRNSLGNLFSVEVLTGMGIDPAMRAENISVAQYCQMANYLAENAPLQES"
     gene            complement(42467..43456)
                     /gene="pdxA"
                     /locus_tag="NBI74_000581"
     CDS             complement(42467..43456)
                     /gene="pdxA"
                     /locus_tag="NBI74_000581"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HCD1930957.1"
                     /translation="MVKTQRVVITPGEPAGIGPDLIVQLAQREWPVELVVCADATLLT
                     DRAAMLGLPLTLRPYSPNSPAQPQTAGTLTLLPVALRESVTVGLLAVENGHYVVETLA
                     RACDGCLNGEFAALITGPVHKGVINDAGIPFTGHTEFFEERSQAKKVVMMLATEELRV
                     ALATTHLPLRDIADAITPALLHEVIAILHHDLRTKFGIAEPRILVCGLNPHAGEGGHM
                     GTEEIDTIIPVLDELREQGMKLNGPLPADTLFQPKYLDNADAVLAMYHDQGLPVLKYQ
                     GFGRGVNITLGLPFIRTSVDHGTALELAGRGKADVGSFITALNLAIKMIVNTQ"
     gene            complement(43456..44742)
                     /gene="surA"
                     /locus_tag="NBI74_000582"
     CDS             complement(43456..44742)
                     /gene="surA"
                     /locus_tag="NBI74_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase SurA"
                     /protein_id="HCD1930958.1"
                     /translation="MKNWKTLLLGIAMIANTSFAAPQVVDKVAAVVNNGVVLESDVDG
                     LMQSVKLNAAQARQQLPDDATLRHQIMERLIMDQIILQMGQKMGVKISDEQLDQAIAN
                     IAKQNNMTLDQMRSRLAYDGLNYNTYRNQIRKEMIISEVRNNEVRRRITILPQEVESL
                     AQQVGNQNDASTELNLSHILIPLPENPTSDQVNEAESQARAIVDQARNGADFGKLAIA
                     HSADQQALNGGQMGWGRIQELPGIFAQALSTAKKGDIVGPIRSGVGFHILKVNDLRGE
                     SKNISVTEVHARHILLKPSPIMTDEQARVKLEQIAADIKSGKTTFAAAAKEFSQDPGS
                     ANQGGDLGWATPDIFDPAFRDALTRLNKGQMSAPVHSSFGWHLIELLDTRNVDKTDAA
                     QKDRAYRMLMNRKFSEEAASWMQEQRASAYVKILSN"
     gene            complement(44795..47149)
                     /gene="lptD"
                     /locus_tag="NBI74_000583"
     CDS             complement(44795..47149)
                     /gene="lptD"
                     /locus_tag="NBI74_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HCD1930959.1"
                     /translation="MKKRIPTLLATMIATALYSQQGLAADLASQCMLGVPSYDRPLVQ
                     GDTNDLPVTINADHAKGDYPDDAVFTGSVDIMQGNSRLQADEVQLHQKEAPGQPEPVR
                     TVDALGNVHYDDNQVILKGPKGWANLNTKDTNVWEGDYQMVGRQGRGKADLMKQRGEN
                     RYTILDNGSFTSCLPGSDTWSVVGSEIIHDREEQVAEIWNARFKVGPVPIFYSPYLQL
                     PVGDKRRSGFLIPNAKYTTTNYFEFYLPYYWNIAPNMDATITPHYMHRRGNIMWENEF
                     RYLSQAGAGLMELDYLPSDKVYKDEHPNDDSSRRWLFYWNHSGVMDQVWRFNVDYTKV
                     SDPSYFNDFDNKYGSSTDGYATQKFSVGYAVQNFNATVSTKQFQVFSEQNTSSYSAEP
                     QLDVNYYQNDVGPFDTRIYGQAVHFVNTRDDMPEATRVHLEPTINLPLSNNWGSINTE
                     AKLLATHYQQTNLDWYNSRNTTRLAESANRVMPQFKVDGRMVFERDMEMLAPGYTQTL
                     EPRAQYLYVPYRDQSKIYNYDSSLLQSDYSGLFRDRTYGGLDRIASANQVTTGVTSRI
                     YDDAAVERFNISVGQIYYFTESRTGDDNITWENDDKTGSLVWAGDTYWRISDRWGLRG
                     GIQYDTRLDNVATSNSSIEYRRDEDRLVQLNYRYASPEYIQATLPKYYSTAEQYKNGI
                     SQVGAVASWPIADRWSIVGAYYYDTNANKQADSMLGVQYSSCCYAIRVGYERKLNGWD
                     NDKQHAVYDNAIGFNIELRGLSSNYGLGTQEMLRSNILPYQNTL"
     gene            47404..48219
                     /gene="djlA"
                     /locus_tag="NBI74_000584"
     CDS             47404..48219
                     /gene="djlA"
                     /locus_tag="NBI74_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlA"
                     /protein_id="HCD1930960.1"
                     /translation="MQYWGKIIGVAVALLMGGGFWGVVLGLLIGHMFDKARSRKMAWF
                     ANQRERQALFFATTFEVMGHLTKSKGRVTEADIHIASQLMDRMNLHGVSRTAAQNAFR
                     VGKSDNYPLREKMRQFRSVCFGRFDLIRMFLEIQIQAAFADGSLHPNERAVLYVIAEE
                     LGISRAQFDQFLRMMQGGAQFGGGYQQQSGGGNWQQAQRGPTLEDACNVLGVKPTDDA
                     TTIKRAYRKLMSEHHPDKLVAKGLPPEMMEMAKQKAQEIQQAYELIKQQKGFK"
     gene            complement(48369..49028)
                     /gene="rluA"
                     /locus_tag="NBI74_000585"
     CDS             complement(48369..49028)
                     /gene="rluA"
                     /locus_tag="NBI74_000585"
                     /EC_number="5.4.99.28"
                     /EC_number="5.4.99.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA pseudouridine(32) synthase/23S
                     rRNA pseudouridine(746) synthase RluA"
                     /protein_id="HCD1930961.1"
                     /translation="MGMENYNPPQEPWLVILYQDDHIMVVNKPSGLLSVPGRLEEHKD
                     SVMTRIQRDYPQAESVHRLDMATSGVIVVALTKAAERELKRQFREREPKKQYVARVWG
                     HPSPAEGLVDLPLICDWPNRPKQKVCYETGKPAQTEYEVVEYAADNTARVVLKPITGR
                     SHQLRVHMLALGHPILGDRFYASPEARAMAPRLLLHAEMLTITHPAYGNSMTFKAPAD
                     F"
     gene            complement(49040..51946)
                     /gene="rapA"
                     /locus_tag="NBI74_000586"
     CDS             complement(49040..51946)
                     /gene="rapA"
                     /locus_tag="NBI74_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001117023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-associated protein RapA"
                     /protein_id="HCD1930962.1"
                     /translation="MPFTLGQRWISDTESELGLGTVVAVDARTVTLLFPSTGENRLYA
                     RSDSPVTRVMFNPGDTITSHDGWQMQVEEVKEENGLLTYIGTRLDTEESGVALREVFL
                     DSKLVFSKPQDRLFAGQIDRMDRFALRYRARKYSSEQFRMPYSGLRGQRTSLIPHQLN
                     IAHDVGRRHAPRVLLADEVGLGKTIEAGMILHQQLLSGAAERVLIIVPETLQHQWLVE
                     MLRRFNLRFALFDDERYAEAQHDAYNPFDTEQLVICSLDFARRSKQRLEHLCEAEWDL
                     LVVDEAHHLVWSEDAPSREYQAIEQLAEHVPGVLLLTATPEQLGMESHFARLRLLDPN
                     RFHDFAQFVEEQKNYRPVADAVAMLLAGNKLSNDELNMLGEMIGEQDIEPLLQAANSD
                     SEDAQSARQELVSMLMDRHGTSRVLFRNTRNGVKGFPKRELHTIKLPLPTQYQTAIKV
                     SGIMGARKSAEDRARDMLYPERIYQEFEGDNATWWNFDPRVEWLMGYLTSHRSQKVLV
                     ICAKAATALQLEQVLREREGIRAAVFHEGMSIIERDRAAAWFAEEDTGAQVLLCSEIG
                     SEGRNFQFASHMVMFDLPFNPDLLEQRIGRLDRIGQAHDIQIHVPYLEKTAQSVLVRW
                     YHEGLDAFEHTCPTGRTIYDSVYNDLINYLASPDETEGFDDLIKNCREQHEALKAQLE
                     QGRDRLLEIHSNGGEKAQALAESIEEQDDDTNLIAFAMNLFDIIGINQDDRGDNMIVL
                     TPSDHMLVPDFPGLSEDGITITFDREVALAREDAQFITWEHPLIRNGLDLILSGDTGS
                     STISLLKNKALPVGTLLVELIYVVEAQAPKQLQLNRFLPPTPVRMLLDKNGNNLAAQV
                     EFETFNRQLNAVNRHTGSKLVNAVQQDVHAILQLGEAQIEKSARALIDAARNEADEKL
                     SAELSRLEALRAVNPNIRDDELTAIESNRQQVMESLDQAGWRLDALRLIVVTHQ"
     gene            complement(52111..54462)
                     /gene="polB"
                     /locus_tag="NBI74_000587"
     CDS             complement(52111..54462)
                     /gene="polB"
                     /locus_tag="NBI74_000587"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase II"
                     /protein_id="HCD1930963.1"
                     /translation="MAQAGFILTRHWRDTPQGTEVSFWLATDNGPLQVFLAPQESVAF
                     IPADQVPRAQHILQGEQGFRLTPLALKDFHRQPVYGLYCRAHRQLMNYEKRLREGGVT
                     VYEADVRPPERYLMERFITSPVWVEGDMRNGAIVNARLKPHPDYRPPLKWVSIDIETT
                     RHGELYCIGLEGCGQRIVYMLGPENGDVSALDFELEYVASRPQLLEKLNVWFATHDPD
                     VIIGWNVVQFDLRMLQKHAERYRIPLRLGRDNSELEWREHGFKNGVFFAQVKGRLIID
                     GIEALKSAFWNFSSFSLETVAQELLGEGKSIDNPWDRMDEIDRRFAEDKPALATYNLK
                     DCELVTQIFHKTEIMPFLLERATVNGLPVDRHGGSVAAFGHLYFPRMHRAGYVAPNLG
                     EVPPHASPGGYVMDSRPGLYDSVLVLDYKSLYPSIIRTFLIDPVGLVEGMAQPDPEHS
                     TEGFLDAWFSREKHCLPEIVTNIWHGRDEAKRQGNKPLSQALKIIMNAFYGVLGTTAC
                     RFFDPRLASSITMRGHQIMRQTKTLIEAQGYDVIYGDTDSTFVWLKGAHSEEEAAKIG
                     RALVQHVNAWWADTLQKQRLTSALELEYETHFCRFLMPTIRGADTGSKKRYAGLIQEG
                     DKQRMVFKGLETVRTDWTPLAQQFQQELYLRIFRNEPYQEYVRETIDKLMAGELDARL
                     VYRKRLRRPLSEYQRNVPPHVRAARLADEENHKRGRPLQYQNRGTIKYVWTTNGPEPL
                     DYQRSPLDYEHYLTRQLQPVAEGILPFIEDNFATLMTGQLGLF"
     gene            complement(54537..55232)
                     /gene="araD"
                     /locus_tag="NBI74_000588"
     CDS             complement(54537..55232)
                     /gene="araD"
                     /locus_tag="NBI74_000588"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HCD1930964.1"
                     /translation="MLEDLKRQVLEANLALPKHNLVTLTWGNVSAVDRGRGVFVIKPS
                     GVDYSIMTADDMVVVSIETGEVVEGTKKPSSDTPTHRLLYQAFPSIGGIVHTHSRHAT
                     IWAQAGQSIPATGTTHADYFYGPIPCTRKMTDAEINGEYEWETGNVIVETFEKQGIDA
                     AQMPGVLVHSHGPFAWGKNAEDAVHNAIVLEEVAYMGIFCRQLAPQLPDMQQTLLDKH
                     YLRKHGAKAYYGQ"
     gene            complement(55432..56934)
                     /gene="araA"
                     /locus_tag="NBI74_000589"
     CDS             complement(55432..56934)
                     /gene="araA"
                     /locus_tag="NBI74_000589"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HCD1930965.1"
                     /translation="MTIFDNYEVWFVIGSQHLYGPETLRQVTQHAEHVVNALNTEAKL
                     PCKLVLKPLGTTPDEITAICRDANYDDRCAGLVVWLHTFSPAKMWINGLTMLNKPLLQ
                     FHTQFNAALPWDSIDMDFMNLNQTAHGGREFGFIGTRMRQQHAVVTGHWQDKQAHERI
                     GSWMRQAVSKQDTRHLKVCRFGDNMREVAVTDGDKVAAQIKFGFSVNTWAVGDLVQVV
                     NSISDGDVNALVDEYESCYTMTPATQIHGEKRQNVLEAARIELGMKRFLEQGGFHAFT
                     TTFEDLHGLKQLPGLAVQRLMQQGYGFAGEGDWKTAALLRIMKVMSTGLQGGTSFMED
                     YTYHFEKGNDLVLGSHMLEVCPSIAVEEKPILDVQHLGIGGKDDPARLIFNTQTGPAI
                     VASLIDLGDRYRLLVNCIDTVKTPHSLPKLPVANALWKAQPDLPTASEAWILAGGAHH
                     TVFSHALNLNDMRQFAEMHDIEITVIDNDTRLPAFKDALRWNEVYYGFRR"
     gene            complement(56945..58645)
                     /gene="araB"
                     /locus_tag="NBI74_000590"
     CDS             complement(56945..58645)
                     /gene="araB"
                     /locus_tag="NBI74_000590"
                     /EC_number="2.7.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulokinase"
                     /protein_id="HCD1930966.1"
                     /translation="MAIAIGLDFGSDSVRALAVECATGEEIATSVEWYPRWQNGQFCD
                     APNNQFRHHPRDYIESMEAALKTVLAELSVEQRAAVVGIGVDTTGSTPAPIDADGNVL
                     ALRPEFAENPNAMFVLWKDHTAVEEAEEITRLCHTPGNVDYSRYIGGIYSSEWFWAKI
                     LHVTRQDNAVAQSAASWIELCDWVPALLSGTTRPQDIRRGRCSAGHKSLWHESWGGLP
                     PASFFDELDPILNRHLPSPLFTDTWTADIPVGTLCPEWAQRLGLPESVVISGGAFDCH
                     MGAVGAGAQPNALVKVIGTSTCDILIADKQSVGERAVKGICGQVDGSVVPGFIGLEAG
                     QSAFGDIYAWFGRVLGWPLEQLAAQHPELKEQINASQKQLLPALTEAWAQNPSLDHLP
                     VVLDWFNGRRTPNANQRLKGVITDLNLATDAPLLFGGLIAATAFGARAIMECFTDQGI
                     AVNNVMALGGIARKNQVIMQACCDVLNRPLQIVASDQCCALGAAIFAAVAAKVHADIP
                     SAQQKMASAVEKTLQPRSEQAQRFEQLYRRYQQWAISVEQHYLPTSAPAQAAQAVPTL
                     "
     gene            58984..59829
                     /gene="araC"
                     /locus_tag="NBI74_000591"
     CDS             58984..59829
                     /gene="araC"
                     /locus_tag="NBI74_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose operon transcriptional regulator AraC"
                     /protein_id="HCD1930967.1"
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKG
                     YILNLTIRGQGIVKNQGREFACRPGDILLFPPGEIHHYGRHPEAHEWYHQWVYFRPRA
                     YWHEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLL
                     LRRMEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQ
                     LGISVLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEF
                     RAGCE"
BASE COUNT        14059 a        15104 c        15995 g        14701 t
ORIGIN      
        1 agacgacttc ttattgcgtg taccctgata acagctatct atcattttcc tgcatattct
       61 tcattagaat ataaaggaac ctttggttca ataaatgcgg gttatgcaga ctggaatagt
      121 ggatttgtaa acactcaccg tggtgaagta tggaaagtga ctgcggattt tggggtaaat
      181 tttaaagaag cagaatttta ctcattttat gaaagtaatg tactcaatca tgctgtagca
      241 gggcgaaatc atacggtttc agtaatgacg catgtcagac tctttgactc tgatatgaca
      301 ttctttggca aaatttatgg ccaatgggat aactcatggg gtgacgatct ggacatgttt
      361 tatggattcg gttacctcgg ctggaacggc gagtggggct tttttaaacc gtatattgga
      421 ttacataatc aatctggtga ctacgtatca gctaaatatg gtcaaacgaa tggttggaat
      481 ggttatgttg ttggctggac agcagtatta ccatttacgt tatttgacga aaaatttgtt
      541 ttatctaact ggaatgaaat agaactggac aggaacgatg cttacacgga gcagcaattt
      601 ggccggaacg ggttaaatgg cggtttaact attgcctgga agttctatcc tcgctggaaa
      661 gccagtgtga cgtggcgtta tttcgataat aagctgggct acgatggctt tggcgatcaa
      721 atgatttata tgcttggtta tgatttctaa ttttttatca gggaataatt atgcagaaaa
      781 cgttaatggc cagtttgatc ggccttgcag tttgcacagg gaatgctttt agtcctgcct
      841 tagccgcaga ggctaaacaa cctaatttag tcattattat ggcggatgat ttaggttatg
      901 gcgatttagc aacatatggt catcagatcg ttaaaacacc taatatcgac aggcttgccc
      961 aggaaggggt caaatttact gactactatg cccccgctcc tttaagttca ccttcacgcg
     1021 cagggctatt aaccggccgg atgccatttc gtactggaat tcgctcatgg attccttcag
     1081 gcaaagatgt tgccttaggg cgtaacgaac tcacgattgc taatctactc aaagcgcaag
     1141 ggtacgacac ggcaatgatg ggtaagctgc atctgaatgc aggcagcgat cgcaccgatc
     1201 agccacaagc acaagatatg ggctttgatt actcactggt taatacggcg ggctttgtta
     1261 ccgacgccac gctggataac gctaaagaac gcccgcgtta tggcatggtt tacccgacag
     1321 gctggctacg taacgggcaa cccactccac gagccgataa aatgagcggt gagtatgtca
     1381 gttcggaagt cgtcaactgg ctggataaca aaaaggacag caagcctttc ttcctctatg
     1441 ttgcttttac cgaagtgcat agccccctgg cttcgcccaa aaaatacctc gatatgtact
     1501 cacaatatat gagcgcgtat cagaagcagc atcctgattt attttatggc gactgggcag
     1561 acaaaccctg gcgtggtgtg ggggaatatt atgccaatat cagctatctg gatgcacagg
     1621 ttggaaaagt gctggataaa atcaaagctg tgggtgaaga agataacaca atcgttattt
     1681 ttaccagtga taacggtccg gtaacgcgtg aagcgcgcaa agtgtatgag ctgaatttgg
     1741 caggggaaac ggatggatta cgcggtcgca aggataacct ttgggaaggc ggaattcgtg
     1801 ttccagccat tattaaatat ggtaaacatc taccacaggg aatggtttca gatacacccg
     1861 tttatggtct ggactggatg cctactttag cgaaaatgat gaacttcaaa ttacctacag
     1921 accgtacttt cgatggtgaa tcgctggttc ctgttcttga gcaaaaagca ttgaaacgcg
     1981 aaaagccatt aattttcggg attgatatgc cattccagga tgatccaacc gatgaatggg
     2041 cgatccgtga tggtgactgg aagatgatta tcgatcgcaa taataaaccg aaatatctct
     2101 acaatctgaa atctgatcgt tatgaaacac ttaatctgat cggtaaaaaa ccagatattg
     2161 aaaaacagat gtatggtaag tttttaaaat ataaaactga tattgataat gattctctaa
     2221 tgaaagccag aggtgataaa ccagaagcgg tgacctgggg ctaacctgat aatcataata
     2281 aatgtaatgt atgccattta gggctatgcc cgcctgattc agtgctacgc attgaatcag
     2341 gttcttttcg cgttgaacaa tatcaagata cggctattcg cagggagtgg atatgtttac
     2401 caacgtaaat gttgattgtt gcaaaacacc aggatgtaaa aacctggggt tgctgaatag
     2461 tcaggattat gtcgcacagg gtaaaaatat tttatgccgt gaatgtggtt acttgtttcc
     2521 agtgatatct gaacagtcgc ttaatattta tcgtaatatt gtgaatcact cctggagagg
     2581 tttgatttgc caatgttcaa cttgcggagg cacgtccctc aaaaaatatg gatattctgc
     2641 acaaggccag agaagaatgt attgccatca ttgtgagaaa acatttatca ctctggaaca
     2701 tgtaattacc acaccacgag gagcccagtt agcattgatg attgagcaag gggaggcact
     2761 tgcggatatc agaaagtcat tacttcttaa cagcactgga cttagccgtg aactgttaaa
     2821 attagcgcgt gaagcaaact ataaagaaag tcgacagtgt ttcccagctt ctgatattac
     2881 cctgagtacc cgcgcttttc gcgtcaagta taatggtagc aataactctc tttatgctct
     2941 tgttaccgca gaagaacaaa gcggcagggt ggttgccatc tcaaccaatt actccccatc
     3001 tgccgtagag caacattatc aatacacatc gaactatgaa gagcgtatgt ctccagggac
     3061 gctggcacat catgtccagc gcaaagagtt acttactatg cggcgggata ccttgtttga
     3121 tattgattac ggcccggcag ttttacatca aaacgatccg ggaatgttgg taaaaccggt
     3181 tcttccggca tatcgtcatt ttgaactggt cagaatactg accgatgagc attccaacaa
     3241 cgttcagcat taccttgatc acgaatgctt tatattgggc ggctgcctga tggctaattt
     3301 gcagcatatt catcaaggtc gctgccatat ttcctttgtc aaagagcgcg gtgtggcacc
     3361 cgccaccatt gattttccgc cgcgattatt ccttagtggt ggggtacgaa ataatgtctg
     3421 gcgtgcattt tctaaccgca attattcaat ggctgtatgc aatctcactg gcagtaagaa
     3481 agtccgcgag atgcggcatg caacattgaa cagtgcgacg cgttttatcc actttgtgga
     3541 gaaccatcct ttccttatat cattgaaccg aatgtctcct gcgaatgtcg tttctacatt
     3601 agatatcctc aaacatctgt ggaataaaaa actagagcat ggaacaattt aattattact
     3661 tttccgaata atttcttcag aaatcttgac attagactat gattaatggg gtttaaggtc
     3721 atcagccgcc gctttcgggc atcaaaaact catctctcgc tgatggcgca attcttttaa
     3781 tagatcgtaa aaagcgagct attcctacac tataatctga ttttaacgat gattcgtgcg
     3841 gggtaaaatc gtgaaaacga tctattcacc tgaagagaaa taaaaagtga aacatctgca
     3901 tcgattcttt agcagtgatg cctcgggagg cattattctc attattgccg ctgtattagc
     3961 gatgattatg gccaacagcg gtgcaaccag tggatggtat cacgactttc ttgagacgcc
     4021 ggttcagctc cgggttggga cacttgagat caacaagaac atgctgctat ggatcaatga
     4081 cgctctgatg gcggtatttt tcctgttggt tggtctggaa gttaaacgcg agctgatgca
     4141 aggttcgctg gccagtctgc gccaggcggc atttcctgtt attgccgcaa tcggcgggat
     4201 gattgtcccg gcattgctct atctggcttt taactatgcc gatcctatca ctcgcgaagg
     4261 ctgggcaatc ccggcggcga ctgacattgc ctttgcactt ggtgtgttgg cgctgttggg
     4321 aagtcgtgtt ccgttagcgc tgaagatctt tttgatggct ctggctatta tcgacgatct
     4381 tggggccatc attatcatcg cattgttcta cactaatgac ttatcgatgg cctctcttgg
     4441 cgtcgcggct gtagcaattg cggtactcgc ggtattgaat ctgtgtggtg tacgccgcac
     4501 gggcgtttat attctggttg gcgtggtgct gtggacagcg gtgttgaaat caggtgtcca
     4561 tgcaacgctg gctggcgtca ttgtcggctt ctttattccg ttaaaagaga agcatgggcg
     4621 ctctccggct aaacgtctgg agcatgtttt gcatccatgg gtggcgtatc tgattttgcc
     4681 gctgtttgca tttgctaatg ctggcgtttc actgcaaggt gtcacgctgg aaggtttgac
     4741 ctccattctg ccattaggga tcatcgctgg tttgctgatt ggcaagccac tgggtattag
     4801 tctgttctgc tggttggcgc tgcgtttgaa actggcacat ctgccagagg gaacgactta
     4861 ccagcaaatt atggcggttg gtatcctgtg cggtatcggt tttactatgt ctatctttat
     4921 tgccagcctg gcatttggta gcgtagatcc agaactgatt aactgggcaa aattaggtat
     4981 ccttgtcggt tcaatttctt cggcggtaat tggatatagc tggttacgcg ttcgtttacg
     5041 tccatcagtt tgacaggacg atgtactggg gagccataaa cggctccctt ttcattgtta
     5101 tcagggagag aaatgagcat gtctcatatc aattacaacc acttgtatta cttctggcat
     5161 gtctacaaag aaggttctgt ggttggcgca gcggaggcgc tttatttaac accacaaacc
     5221 attaccgggc agatccgggc gctggaagag cgcctgcaag ggaaactatt taagcgtaaa
     5281 ggacgtggtc tggaacccag cgaactgggg gaactggtct atcgctatgc cgataaaatg
     5341 ttcaccttaa gccaggaaat gctggatatc gtcaactatc gcaaagagtc caacttattg
     5401 tttgatgttg gtgtggcaga tgcactttcc aaacgtctgg tcagtagcgt acttaacgcc
     5461 gcagtggtag aaggcgagcc gattcatctt cgctgcttcg aatccaccca cgaaatgctg
     5521 ctggagcaat taagtcagca taaactggat atgatcattt ccgactgtcc gatagattct
     5581 acgcagcagg aaggcctgtt ctccgtgaga attggcgaat gtggcgtgag tttctggtgt
     5641 acaaatccac caccggaaaa accgttcccg gcttgtctgg aagaacggcg gcttttgatt
     5701 cctgggcgac gttcaatgtt agggcgcaaa ttgcttaact ggtttaactc ccagggatta
     5761 aacgtagaaa tccttggcga gtttgatgat gccgcgttga tgaaagcttt tggtgcgatg
     5821 cacaatgcaa tcttcgttgc tccaacgctt tatgcatatg acttttatgc cgataaaact
     5881 gtcgtagaaa ttggtcgcgt cgagaatgtg atggaagagt accatgctat ttttgctgag
     5941 cggatgattc agcacccggc ggtacagcga atctgcaata cggattattc ttcgttgttt
     6001 tctcccgcct cacgatagct attaaaactc acgccccgct acatacgggg cgtgctaatg
     6061 aacaaacgat gtcctttaaa cgggaaatat ctggcgatat ttccctttct cgcgaatgaa
     6121 taccggtatc tggtgcagcg gtgcgcaatg atgaagcgtt tgcccacccg cgtagtgttc
     6181 atgggtataa aaatcgatcc atgtctctcc agcaggcaac cagacatcac gtgaacgtgt
     6241 accttcgtgc attacagggg caacgagaag gtctgggcca aagcagtact gatcataaat
     6301 attccagctt tctgcctggt caggaaattc aaagaacatc gtgcgcatta tcgggctgtt
     6361 cttctcgtgc gtctcagcca taattttact gatataagat ttcagttttt cacgcaactg
     6421 caggcaggaa gtgagaattt caccggtttc ttcaccatag ctccagactt cattcggcgc
     6481 gccagtcgga cattgtgcta tgccatcacg atagggttgt tcaggcaaaa cctgcgggtc
     6541 gcggttgcca tgtaaacgca ttactgggct gaataccccc cactggaacc agcgaattaa
     6601 cagttcatgg aatttcggat cgtgaatatt accgccgtgg aaaccaccaa tatcggttgt
     6661 ccaccacggg atcccggcaa ttcccatgtt caagccagcg gcaaactgat tacgcaaaga
     6721 acggaaagag gagtgaatat cgcctgacca caccagagcg ccatatttct gactgccagc
     6781 ccaggcacag cgcagcaggt taatcacctg cttttcacca gccgcggtca taccatcgaa
     6841 gaacgttttg gcgtacatac gcggatagat attaccgact tccagcacag gccctgcgta
     6901 gtaacgatag ttgtcataat cgtaaacacc gaactctggc tcggcttcat ccagccagaa
     6961 cagtttcacg cctttatcgt aataattgcg tttggctttg ttccagacat attcccgtgc
     7021 gccaggatgg gtagcatcaa agaacgtggt gttgccaagg aaatccatat tgataggcaa
     7081 accacgctcg gtgtggacca gccagccgtt ttctttcatc tcccgataac tctcggtacg
     7141 gttatcaacc gtgggccaga aggagaccat cagttcaatg cccatctctt ttagttcggc
     7201 aatcatggca tcgggatcgg gccagtcgcg cagatcgaac atccagtcac cctgattcgg
     7261 ccaatggaag aaatcgatga caatcaccga aataggcaaa ttacgctgct tgtagccacg
     7321 tgcaacttct agtaactctt gctgattacg gtagcgcaac ttgcactgcc agaatcccat
     7381 ggcgtaatcg ggcatcattg gcggcgtacc ggtaacacgt gcataggcgg cgctgatttc
     7441 tgcgggagta tcccctgcgg taacccagta gtccaactgt tcgctaactt ctgccaccca
     7501 ttccgtgcca ttttgcgcga aagtgacgcg acccaccgct gggttattcc acagaaaacc
     7561 atagccgcga ttagaaagca ggaagggaac cgatgcctga gaattacgct gcgccagctc
     7621 tagcatacag ccttttaaat ccagatttgg ctgctggtac tgccccatgc caaacaactt
     7681 ttcgccatcg ttggcttcga accgcgcctt gattgtgtat ttcccgccgg gaatcggttt
     7741 gaactcgcgg gcctgtaaat tcagggcgct gacatactga ccgtgggccg actcatcttc
     7801 gccaatttcg ccgcgctgac gccagaactc ctgcagcagg catttttctg cattacgcca
     7861 gaaagagatc tgaccctgca gattaacaat ggcggtgatt ttgccgtttt gtagtgttgc
     7921 ctgcttttct tcatatgtga tataggcgac agcatcacct ttttcttctg ttaatgccca
     7981 gttttcattg cgcatcatcg gcagatggcg accgctacgc acacgtaaac tgttctcacc
     8041 ccatgcttca atccagagat aacgatcgtc ttgttgccag accagacgat ggggatcttg
     8101 cttaataaac gacataattt cctccaggag cttgtgacat gtttaagggt tatgaacttg
     8161 caaatcgtgt tggatgcact cttccatcgc ttcatattta cgaaagaaga gcagtggaat
     8221 ggcggcgagt gcaaagagca cggctggcac ccagataaac gcccagctaa taccgtccag
     8281 accggcggca gtttgtggct ggttgggaat atagccaaag ctgttcataa tccaggcggc
     8341 gaaggcggtg ccgataccac tgcccatctt gatgcagaag gtactgccga aggcgattag
     8401 aatgccggta gctttaatcc ctgttttcca gcgaccaaaa tcaaccgcga aaccaagcat
     8461 ggcgaagggc atagagcagg caatgccact gccaatattc gccagcatcc aggcggcaat
     8521 catcaaagga atattgtctc cagccagcca catcagtcct ccgccgaaca tggcgaccag
     8581 caggccggtg atccaaatcc aggttttgga gaggtaacgg ctgaaaaacg gaattgcgat
     8641 gataaacaaa atctgaatgg tggcgaggct gttgatcaag ggaactaaat ctttgcgatc
     8701 caggttgtag gttaaatagt aaacaatggt tgtgttgcgc tgctgtaagg caatccagaa
     8761 gatgagattt gccagcacca tcaggatcca cggccagttg cctttcattg ccgaaaagct
     8821 ctttttcatc ggcagacgtg gttcctgctg tattttgtcg gtatccagtt ctcgaatatt
     8881 tttgaaggca aatagcgtta gcgcacagga gattacggaa aagacgatag cagtataaat
     8941 aaatcctgcc ttatcattac cgtttccaag aaaagccact aacggtagcg ccgtcgcatt
     9001 cattaataac acgccaattt gcccgccggt catccggtaa gagtttaata ccaaccgttc
     9061 ttttggtgat aacgtcatca aaggtaatat tgcgcttagc ggcgtgttta aaccggtaaa
     9121 aagaatgctg gctaacatgt acgatattgc tgaatatatg gttttacctg tcatactgat
     9181 atctggcgac cagaatgaga gtgcgctaaa tacagcaaaa ggtaatggca accataaaaa
     9241 ccacggccga catttaccgt agcgggaatg cgttttatca ataataatcc cccataccgg
     9301 ggcatcgata ccgtctacaa tacgcgccag tagcaggatg atgccagcta cggcaaggct
     9361 tatgcccgcc acgtcagtgt agaaatagag aatgtaggtg gagccaaaac agtagagcag
     9421 attacctgca acatccagcg aaccatagct gatccgttgt attaaaggca gtgaatcgga
     9481 tgcggatgat ttatcagttt caagtacgga actcattata ttctctccag aacaagaatg
     9541 cctggctcag aaaaacgact tttactttct aagcaaatac atgctaacta tccttgatgc
     9601 gtatttatta atgatgtaaa gcaacgttgt ggcaatgaga gtaattaaag tggggaagag
     9661 cgacaattat aatttttcgt tttaaggttg cgttaattag ttttttattc agttttcctg
     9721 ctatttgtta tacagttcac gttttacagc caaatgaaat attttatctt tcacaatgaa
     9781 aaagcatcaa ttatatttat gatgtattca tggagaaaat atataatcga ttaacggtaa
     9841 cttccgcagg cataaaaaaa cccgccagag cgggtttttt tacaaagctg acaacaacag
     9901 gcaattaagc cagtttgttg atctgtgcag tcaggttagc cttatgacgt gcagctttgt
     9961 ttttgtggat cagaccttta gcagcctgac ggtccacgat tggttgcatt tcgttaaatg
    10021 ctttctgtgc agcagctttg tcgccagctt cgatagctgc gtatactttc ttgatgaaag
    10081 tacgcatcat agagcgacgg cttgcgttgt gcttacgagc cttttcagac tgaatggcgc
    10141 gcttcttagc tgatttgata ttagccaagg tccaactccc aaatgtgttc tatatggaca
    10201 attcaaaggc cgaggaatat gcccttttag ccttcttttg tcaatggatt tgtgcaaata
    10261 agcgccgtta atgtgccggc actcgttacg tagtgatggc gcaggattct accagcttgc
    10321 ggggtgtgaa tacagctttt ccgcgagaaa aattgcagta ggcggtcagt ttcttcccat
    10381 gatttgcgcc atagcaatga aaagccactt ctttctgatt tcggtactca atcgccggtt
    10441 aaccttgacc gctgtacaag gtatactcgg acgattttca ctgttttgag ccagacatga
    10501 agctgatacg cggcatacat aatctcagcc aggccccgca agaagggtgt gtgctgacta
    10561 ttggtaattt cgacggcgtg catcgcggtc atcgcgcgct gttacagggc ttgcaggaag
    10621 aagggcgcaa gcgcaactta ccggtgatgg tgatgctttt tgaacctcaa ccactggaac
    10681 tgtttgctac tgataaagcc ccggcacggc tgacccggct gcgggaaaaa ctgcgttatc
    10741 ttgcagagtg tggcgttgat tacgtgctgt gcgtgcgttt tgacaggcgt tttgcggcgt
    10801 taaccgcgca aaacttcatc agtgatcttc tggtgaagca cttgcgggta aaatttcttg
    10861 ccgtaggtga cgatttccgc tttggcgctg gtcgtgaagg cgatttcttg ttattacaga
    10921 aagcgggtat ggaatacggc ttcgatatca ccagcacgca aactttttgc gaaggtggcg
    10981 tgcgtatcag cagcaccgcc gtgcgtcagg cgctggcgga tgacaatctg gctctggcag
    11041 aaagtttact ggggcacccg tttgccatct ccgggcgtgt agtccacggt gatgaattag
    11101 ggcgcactat aggtttcccg acggcgaatg taccgctacg ccgtcaggtt tccccggtga
    11161 aaggggttta tgcggtagaa gtgttgggcc ttggcgaaaa gccgttaccc ggcgttgcaa
    11221 acatcggaac acgcccaacg gttgccggta ttcgccagca actggaagtg catttgttag
    11281 atgttgcaat ggacctttat ggtcgccata tacaagtagt gctgcgtaaa aaaatacgca
    11341 atgagcagcg atttgcatcg ctggacgaac tgaaagcgca gattgcgcgt gatgaattaa
    11401 ccgcccgcga attttttggg ctaacaaaac cggcttaagc ctgttatgta atcaaaccga
    11461 aatacggaac cgagaatctg atgagtgact ataaatcaac cctgaatttg ccggaaacag
    11521 ggttcccgat gcgtggcgat ctcgccaagc gcgaaccggg aatgctggcg cgttggactg
    11581 atgatgatct gtacggcatc attcgtgcag ctaaaaaagg caaaaaaacc ttcattctgc
    11641 atgatggccc tccttatgcg aatggcagca ttcatattgg tcactcggtt aacaagattc
    11701 tgaaagacat tatcattaag tccaaagggc tttctggata tgactcgccg tatgtgcctg
    11761 gctgggactg tcatggtctg ccaatcgaac tgaaagtaga gcaagaatac ggtaagccgg
    11821 gggagaaatt caccgccgct gagttccgcg ccaagtgccg cgaatacgct gcgacccagg
    11881 ttgacggtca gcgcaaagac tttatccgtc tgggcgtgct gggcgactgg tcgcacccgt
    11941 acctgaccat ggacttcaaa actgaagcca acatcatccg cgcgctgggc aaaatcatcg
    12001 gcaacggtca cctgcacaaa ggcgcgaagc cggtgcactg gtgcgttgac tgccgttctg
    12061 cactggcaga agcggaagtt gagtattacg acaaaacttc tccgtccatc gacgtcgctt
    12121 tccaggcggt cgatcaggat gcgctgaaaa cgaaatttgg cgtaagcaat gttaacggcc
    12181 caatttcgct ggttatatgg accaccacgc cgtggacgct gcctgctaac cgcgcaatct
    12241 ccattgcacc tgattttgat tatgcgctgg tgcaaatcga cggtcaggcc gtgattctgg
    12301 cgaaagatct ggttgaaagc gtaatgcagc gtatcggcgt tagcgattac accattcttg
    12361 gcacggtgaa aggtgctgag ctggaactgt tgcgctttac ccatccgttt atggacttcg
    12421 atgttccggc aattcttggc gaccacgtta cgctggatgc cggtaccggt gccgttcata
    12481 ccgcgccagg ccacggtccg gacgactatg tgatcggtca aaaatatggt ctggaaaccg
    12541 ctaacccggt tggcccggac ggcacttatc tgccgggtac ttacccgact ctggatggcg
    12601 ttaacgtctt caaagcgaac gatattgtca ttgcgttgtt gcaggaaaaa ggcgcactgt
    12661 tgcacgttga gaaaatgcaa cacagctatc cgtgctgctg gcgtcataaa acgccgatca
    12721 tcttccgcgc gacgccgcag tggttcgtca gcatggatca gaaaggtctg cgtgcgcagt
    12781 cactgaaaga gatcaaaggc gtgcagtgga tccctgactg gggccaggcg cgtatcgagt
    12841 cgatggttgc taaccgtcct gactggtgta tctctcgtca gcgtacctgg ggcgtgccga
    12901 tgtcactgtt cgtgcacaaa gacacagaag aactgcatcc gcgtactctc gaactgatgg
    12961 aagaagtggc aaaacgcgtt gaagttgacg gcatccaggc gtggtgggat ctcgatgcga
    13021 aagagatcct cggcgacgaa gctgaccagt atgtgaaagt accggatacg ctggatgtat
    13081 ggtttgactc cggatctacc cactcttccg ttgttgatgt gcgtccggaa tttgccggtc
    13141 acgcagcgga catgtatctg gaaggttctg accaacaccg tggctggttc atgtcttccc
    13201 tgatgatctc taccgcgatg aagggcaaag cgccatatcg tcaggtactg actcacggct
    13261 ttaccgtgga tggtcagggt cgcaagatgt ctaaatccat cggtaacacc gtttcgccgc
    13321 aggatgtgat gaacaaactg ggtgcggata ttctgcgtct gtgggtggca tcaaccgact
    13381 acaccggcga aatggctgtt tctgacgaga ttctgaaacg tgctgccgac agctatcgtc
    13441 gtatccgtaa caccgcgcgc ttcctgctgg caaacctgaa cggttttgat ccggcaaaag
    13501 atatggtgaa accggaagag atggtggtac tggatcgctg ggccgtaggt tgtgcgaaag
    13561 cggcacagga agacatcctc aaggcgtacg aagcatacga tttccacgaa gtggtacagc
    13621 gtctgatgcg cttctgctcc gttgagatgg gttccttcta cctcgacatc atcaaagacc
    13681 gtcagtatac cgccaaagcg gacagcgtgg cgcgtcgtag ctgccagact gcgctgtatc
    13741 acatcgcaga agcgctggtt cgctggatgg caccaatcct ctccttcacc gctgatgaag
    13801 tgtggggcta cctgccgggc gaacgtgaaa aatacgtctt caccggcgag tggtacgaag
    13861 gcctgtttgg tctggcagac agtgaagcaa tgaacgatgc gttctgggac gagctgttga
    13921 aagtgcgtgg cgaagtgaac aaagtcattg agcaagcgcg tgccgataag aaagtgggcg
    13981 gctcgctgga agctgcagta accttgtatg cagaaccgga actggcggcg aaactgaccg
    14041 cgctgggcga tgaattacga tttgtcctgt tgacctccgg cgctaccgtt gcagactata
    14101 acgacgcacc tgctgatgcc cagcagagcg aagtcctcaa agggctgaaa gtcgcgttga
    14161 gtaaagccga aggtgagaag tgccctcgct gctggcacta cacccaggat gtcggcaagg
    14221 tggcggaaca cgcagaaatc tgcggccgct gtgtcagcaa cgtcgccggt gacggtgaaa
    14281 aacgtaagtt tgcctgatga gtcaatcgat ctgttcaaca gggctacgct ggctgtggct
    14341 ggtggtagtc gtgctgatta tcgatctggg cagcaaatac ctgatcctcc agaactttgc
    14401 tctgggggat acggtcccgc tgttcccgtc gcttaatctg cattatgcgc gtaactatgg
    14461 cgcggcgttt agtttccttg ccgatagcgg cggctggcag cgttggttct ttgccggtat
    14521 tgcgattggt attagcgtga tcctggtagt gatgatgtat cgctcgaagg ccgcgcagaa
    14581 gctaaacaat atcgcttacg cgctgattat tggcggcgcg ctgggcaacc tgttcgaccg
    14641 cctgtggcac ggcttcgttg tcgatatgat cgacttctac gtcggcgact ggcacttcgc
    14701 caccttcaac cttgccgata ctgccatttg tgtcggtgcg gcactgattg tgctggaagg
    14761 ttttttacct tctaaagcga aaaaacaata ataaaccctg ccggatgcga tgctgacgca
    14821 tcttatccgg cctacagatt gctgcgaaat cgtaggccgc atccggcaaa aatcctcaaa
    14881 atataagagc aaacctgcat gtctgaatct gtacagagca atagcgccgt cctggtgcac
    14941 ttcacgctga aactcgatga tggcaccacc gctgagtcta cccgcaacaa cggtaaaccg
    15001 gcgctgttcc gcctgggtga tgcttctctt tctgaagggc tggagcaaca cctgctgggg
    15061 ctgaaagtgg gcgataaaac caccttctcg ctggagccag atgcggcgtt tggcgtgccg
    15121 tcaccggacc tgattcagta cttctcccgc cgtgaattta tggatgcagg cgagccagaa
    15181 attggcgcaa tcatgctttt taccgcaatg gatggcagtg agatgcctgg cgtgatccgc
    15241 gaaattaacg gcgactccat taccgttgat ttcaaccatc cgctggccgg gcagaccgtt
    15301 cattttgata ttgaagtgct ggaaatcgat ccggcactgg aggcgtaaca tgcagatcct
    15361 gttggccaac ccgcgtggtt tttgtgccgg ggtagaccgc gctatcagca ttgttgaaaa
    15421 cgcgctggcc atttacggcg caccgatata tgtccgtcac gaagtggtgc ataaccgcta
    15481 cgtggtcgat agcctgcgcg agcgtggggc tatctttatt gagcagatta gcgaagtacc
    15541 ggacggcgcg atcctgattt tctccgcaca cggtgtttct caggcagtac gtaacgaagc
    15601 gaaaagccgc gatttgacgg tgtttgatgc cacctgtccg ctggtgacca aagtgcatat
    15661 ggaagtcgcc cgcgccagcc gtcgtggtga agaatctatt ctcatcggtc acgccgggca
    15721 cccggaagtg gaagggacga tgggccagta cagcaacccg gaagggggaa tgtatctggt
    15781 cgaatcgcca gacgatgtgt ggaaactgac ggtcaaaaat gaagagaagc tctcctttat
    15841 gacccaaacc acgctgtcgg tggatgacac gtctgatgtg atcgacgcgc tgcgtaaacg
    15901 cttcccgaaa attgtcggtc cgcgcaaaga tgacatctgc tacgccacga ctaaccgtca
    15961 ggaagcggta cgcgccctgg cagagcaggc ggaagtagta ctggtggttg gttcgaaaaa
    16021 ctcctccaac tccaaccgtc tggcggaact ggcccagcgt atgggcaaac gcgcattttt
    16081 gattgacgat gcgaaagata tccaggaaga gtgggtgaaa gaggttaaat gcgtcggcgt
    16141 gactgcgggc gcatcggctc cggatattct ggtgcagaat gtggtggcac gcttgcagca
    16201 gcttggcggt ggcgaagcca ttccgctgga aggccgcgaa gaaaatatcg tcttcgaagt
    16261 gccgaaagag ctgcgtgtcg atattcgtga agtcgattaa gtcattagta gtctaagtta
    16321 tgcgaaaatg ccggtcttgt taccggcatt ttttatggag aaaacatgca tttacctatc
    16381 ttcctcgata cagaccccgg cattgacgat gccgttgcca ttgccgccgc gatttttacc
    16441 cccgaactcg atctgcaact gatgaccaca gtcgcgggta atgtttcggt agagaaaacc
    16501 acccgcaacg ccctgcaact gctgcatttc tggaatgtgg atattccgct tgcccagggc
    16561 gctgccgtac cactggttcg tgcgccgcgc gatgcggcat ctgtacacgg tgaatcggga
    16621 atggctggct ataactttgt tgaacacaac cgtcagccac tgggtatacc ggcgtttctg
    16681 gcaattcgtg atgccctgat gcgtgcgcca gagcccgtta ccctggtggc tatcggtccg
    16741 ttaaccaata ttgcgctgtt actttcacaa tgcccggaat gtaagccgta tattcgccgt
    16801 ctggtgatca tgggcggttc tgctggtcgc ggcaactgca caccaaacgc cgagtttaat
    16861 atcgctgccg atccagaagc ggcagcctgt gtcttccgta gcggcatcga aatcgtcatg
    16921 tgcggtctgg atgtcaccaa tcaggcaata ttaacccctg actatctcgc tactttgccg
    16981 gagttgaatc gcaccggaaa aatgctacac gccctgttta gccactaccg tagcggcagt
    17041 atgcaaagtg gactacgtat gcacgatctt tgcgctatcg cctggctggt gcgcccggaa
    17101 ttgttcacac tcaaaccgtg ttttgtcgcg gtagaaactc agggtgaatt tacctcaggc
    17161 acgacggttg ttgatatcga cggttgcctg ggcaaaccag ccaatgtaca ggtggcgctg
    17221 gatctggatg tgaaaggctt ccagcagtgg gtggctgagg tgctggccct ggcgctctaa
    17281 taataaaaca aggaagtgtt ctatgacgta tggagaagcg tatctggaag ggtggaaaaa
    17341 catctttaat tatgaaggtg tttccaatcg cttcgagttc tggtcgttta tgattggtag
    17401 tggcgttatc tgtctgcttc cgcttttatg ttggtggtta gcggtaacaa ttaacaatga
    17461 ctatggtgtt ttcatttttt ttgcgctgcc cgcgtcattt attttaacgt tgatttttgc
    17521 cgtaccggcg atagcgttag ccgtcagaag gatgcacgat atcggatatt cagggtggtg
    17581 ggttaccatt gtcgtgttga tccctgttac gggcgtaatt ctgcttatac tgtgctgcct
    17641 gccgtcaaag tcacaagacc aggcataatg ccagagggta tgacgcatca ctttctgcgt
    17701 catgcctttc actgatatcc ctccctgttt atcattaatt tctaattatc agcgtttttg
    17761 gctggcggcg tagcgatgcg ctggttactc tgaaaacggt ctatgcaaat taacaaaaga
    17821 gaatagctat gcatgatgca aacatccgcg ttgccatcgc gggagccggg ggccgtatgg
    17881 gccgccagtt gattcaagcg gcgctggcat tagaaggcgt gcagctgggc gcggcgctgg
    17941 agcgcgaagg atcttcttta ctgggcagcg atgcgggcga actggccgga gccgggaaaa
    18001 caggcgttac tgtgcaaagt agtcttgatg cgataaaaga tgattttgat gtgtttatcg
    18061 attttacccg tccggaaggt acgctgaacc atctcgcttt ttgtcgccag catggcaaag
    18121 ggatggtgat cggcactacg gggtttgacg aagccggtaa acaggcgatt cgtgacgccg
    18181 ccgccgatat tgcgattgtc tttgccgcca attttagcgt tggcgttaac gtcatgctta
    18241 aactgctgga gaaagcagcc aaagtgatgg gggactacac cgatatcgaa attattgaag
    18301 cacatcatag acataaagtt gatgcgccgt caggcaccgc actggcaatg ggagaggcga
    18361 tcgcccacgc tcttgataaa gatctgaaag attgcgcggt ctacagtcgt gaaggccata
    18421 ccggtgaacg tgtgcctggc accattggtt ttgccaccgt gcgtgcaggt gacatcgttg
    18481 gcgaacatac cgcgatgttt gccgatattg gcgagcgtct ggagatcacc cataaggcat
    18541 ccagccgcat gacatttgct aacggtgcgg taagatcggc tttgtggttg agtggtaagg
    18601 aaagtggtct ttttgatatg cgagatgtgc ttgatctcaa taatctgtaa ccacaaaata
    18661 tttataatga tgcaaaaata acacatttaa ttcattgaat ataaagggct ttaatttttg
    18721 gcccttttat ttttggtgtt atgtttttaa atttaatgca aatgctgaaa gttacatgtt
    18781 ttgtattctg tttttgttgt tttaatgtaa attttgacca tttggtccac ttttttctac
    18841 tcattttgat ttcatgcaat cttcttgctg cgcaagcgtt ttccagaaca tgttatatga
    18901 tctttttgtc gcttaatgcc tgtaaaacat gcatgagcca caaaataata taaaaaatcc
    18961 cgccattaag ttgactttta gctcccatat ctccagaatg ccgccgtttg ccagaaattc
    19021 gtcggtaagc agatttgcat tgatttacgt catcattgtg aattaatatg caaataaagt
    19081 gagtgaatat tctctggagg gtgttttgat taagtcagcg ctattggttc tggaagacgg
    19141 aacccagttt cacggtcggg ccataggggc aacaggttcg gcggttgggg aagtcgtttt
    19201 caatacttca atgaccggtt atcaagaaat cctcactgat ccttcctatt ctcgtcaaat
    19261 cgttactctt acttatcccc atattggcaa tgtcggcact aatgacgccg atgaagaatc
    19321 ttctcaggta catgcccaag gtctggtgat tcgcgacctg ccgctgattg ccagcaactt
    19381 tcgtaatacc gaagacctct cttcttacct gaagcgccat aacatcgtgg cgattgccga
    19441 tatcgatacc cgtaagctga cgcgtttact gcgcgagaaa ggcgcacaga atggctgcat
    19501 tatcgcaggc gataacccgg atgcggcgct ggcgttagaa aaagcccgcg cgttccctgg
    19561 cctgaatggc atggatctgg caaaagaagt gactaccgca gaaccctata gctggacaca
    19621 agggagctgg acgctgaccg gcggtctgcc agaagcgaaa aaagaagacg agctgccgta
    19681 ccacgtcgtg gcttatgatt ttggtgccaa gcgcaacatc ctgcggatgc tggtggatag
    19741 aggctgtcgt ctgaccatcg ttccggcgca aacttctgcg gaagatgtgc tgaaaatgaa
    19801 tccagacggc atcttcctct ccaacggtcc tggcgacccg gccccgtgcg attacgccat
    19861 taccgccatc cagaaattcc tcgaaaccga tattccggta ttcggcatct gcctcggcca
    19921 tcagctgctg gcgctggcga gcggtgcgaa gactgtcaaa atgaaatttg gtcaccacgg
    19981 cggcaaccat ccggttaaag atgttgagaa aaatgtggtg atgatcaccg cccagaacca
    20041 cggttttgcg gtggatgaag caacattacc tgcaaacctg cgtgtcacac ataaatccct
    20101 gttcgacggt acgttacagg gcattcatcg taccgataaa ccggcgttca gcttccaggg
    20161 acacccggaa gccagccctg gtccacacga cgccgcgccg ttgtttgacc actttatcgc
    20221 gttaattgag cagtaccgta aaaccgctaa gtaatcagga gtaaaagagc catgccaaaa
    20281 cgtacagata taaaaagtat cctgattctg ggtgcgggcc cgattgttat cggtcaggcg
    20341 tgtgagtttg actactctgg cgcgcaagcg tgtaaagccc tgcgtgaaga gggttaccgc
    20401 gtcattctgg tgaactccaa cccggcgacc atcatgaccg acccggaaat ggctgatgca
    20461 acctacatcg agccgattca ctgggaagtg gtacgcaaga ttattgaaaa agagcgcccg
    20521 gacgcggtgc tgccaaccat gggggggcag acggcgctga actgtgcgct ggagcttgag
    20581 cgtcagggcg tgctggaaga gttcggcgtc accatgattg gtgccactgc cgatgcgatt
    20641 gataaagccg aagaccgccg ccgtttcgac gtagcgatga agaaaatcgg tctggaaact
    20701 gcgcgttccg gtatcgctca ttcaatggaa gaagcgctgg cggttgccgc tgaagttggc
    20761 ttcccgtgca ttattcgccc atcctttacc atgggcggta gcggcggcgg tatcgcttat
    20821 aaccgcgaag agtttgaaga aatttgcgcc cgcggtctgg atctttctcc gactaaagag
    20881 ctgctgattg atgagtcgct gatcggctgg aaagagtacg agatggaagt ggtgcgtgat
    20941 aaaaacgaca actgcatcat cgtctgctct atcgaaaact ttgatgcgat gggcatccac
    21001 accggtgact ccatcactgt cgcgccagcc caaacgctga ccgacaaaga atatcaaatc
    21061 atgcgtaacg cctcgatggc ggtactgcgt gaaatcggcg ttgaaaccgg tggttccaac
    21121 gtccagtttg cggtgaaccc gaaaaacggt cgcctgattg ttatcgaaat gaacccacgc
    21181 gtgtctcgct cctctgcgct ggcgtcgaaa gcgaccggct tcccgattgc caaagtggcg
    21241 gcgaaactgg cggtgggtta caccctcgac gagctgatga acgacatcac cggtgggcgt
    21301 actccggcct ccttcgagcc gtccatcgac tatgtggtta ctaaaattcc tcgcttcaac
    21361 ttcgagaaat tcgccggtgc taacgaccgt ctgaccactc agatgaaatc ggttggcgaa
    21421 gtgatggcga ttggtcgcac gcagcaggaa tccctgcaaa aagcgctgcg cggcctggaa
    21481 gtgggcgcaa ctggcttcga tccgaaagtg agtctcgacg acccggaagc gctgactaaa
    21541 atccgccgcg aactgaaaga tgcaggcgca gagcgtatct ggtatatcgc tgatgccttc
    21601 cgcgcgggcc tgtctgtaga cggcgtgttt aatttgacca acattgaccg ctggttcctg
    21661 gtacagattg aagagctggt gcgtctggaa gagaaagtcg cagaagtggg catcactggc
    21721 ctgaacgctg aattcctgcg ccagctgaaa cgcaaaggct ttgccgatgc gcgtctggca
    21781 aaactcgcgg gcgtgcgtga agcagaaatc cgcaagctgc gcgaccagta tgacttgcac
    21841 ccggtttata agcgcgtgga tacctgtgcg gcagagttcg ccaccgacac cgcttacatg
    21901 tactccactt atgaagaaga gtgcgaagcg aatccgtcca ccgatcgtga aaaaatcatg
    21961 gtgctcggcg gcggcccgaa ccgtatcggt cagggtatcg aattcgacta ctgctgtgta
    22021 cacgcctcgc tggcgctgcg cgaagatggt tatgaaacca ttatggttaa ctgtaacccg
    22081 gaaaccgtct ccaccgacta cgacacctcc gaccgtctct acttcgagcc ggtaacgctg
    22141 gaagacgtgc tggaaattgt gcgtattgag aagccgaaag gcgttatcgt ccagtacggc
    22201 ggtcagaccc cgctgaaact ggcgcgcgcg ctggaagctg ctggcgtacc ggttatcggt
    22261 accagcccgg atgccatcga ccgtgcggaa gaccgtgaac gcttccagca tgcggttgac
    22321 cgtctgaaac tgaaacaacc ggcgaacgcc accgttacca ccatcgaaat ggcggttgag
    22381 aaagcgaaag agattggcta cccgctggtg gtgcgtccgt cttacgttct cggcggtcgg
    22441 gcgatggaaa tcgtctatga cgaagctgac ctgcgtcgct acttccagac agcggtcagc
    22501 gtatctaacg atgcgccagt gttgctggac cacttccttg atgacgcagt agaagttgac
    22561 gtggatgcca tctgcgacgg cgaaatggtg ctgattggcg gcatcatgga gcatattgag
    22621 caggcgggtg tgcactccgg tgactccgca tgttcactgc cagcctacac cttaagtcag
    22681 gaaattcagg atgtgatgcg ccagcaggtg cagaaactgg ccttcgaact gcaggtgcgc
    22741 ggcctgatga acgtgcagtt tgcggtgaaa aacaacgaag tctacctgat tgaagttaac
    22801 ccgcgtgcgg cgcgtaccgt tccgttcgtc tccaaagcca ccggcgtacc gctggcaaaa
    22861 gtggcggcgc gtgtgatggc tggcaaatcg ctggctgagc agggcgtaac caaagaagtt
    22921 atcccgccgt actactcggt gaaagaagtg gtgctgccgt tcaataaatt cccgggcgtt
    22981 gacccgctgt tagggccaga aatgcgctct accggggaag taatgggcgt gggccgcacc
    23041 ttcgctgaag cgtttgccaa agcgcagctg ggcagcaact ccaccatgaa gaaacacggt
    23101 cgtgcgctgc tttccgtgcg cgaaggcgat aaagaacgcg tggtggacct ggcggcaaaa
    23161 ctgctgaaac agggcttcga gctggatgcg actcacggca cggcgattgt gctgggcgaa
    23221 gcgggtatca atccgcgtct ggtgaacaag gtgcatgaag gccgtccgca cattcaggac
    23281 cgtattaaga atggcgaata tacctacatc atcaacacca cctcaggtcg tcgtgcgatt
    23341 gaagactccc gcgtgatccg tcgcagtgcg ctgcaatata aagtgcatta cgacaccacc
    23401 ctgaacggcg gctttgccac cgcgatggcg ctgaatgccg atgcgaccga aaaagtaatt
    23461 tcggtgcagg aaatgcacgc gcagatcaaa taatagcgtg tcatggcgga tatttttcat
    23521 ccgctaattt gatcaaataa ctaatacggt tctctgatga ggaccgtttt ttttgcccat
    23581 taagtaaatc ttttggggaa tcgatatttt tgatgacata agcaggattt agctcacact
    23641 tatcgacggt gaagttgcat actatcgata tatccacaat tttaatatgg ccttgtttaa
    23701 ttgctgcaaa acgagtcata gccagtcttt taatttgtga aactggagtt cgtatgtgtg
    23761 aaggatatgt tgaaaaacca ctctacttgt taatcgccga atggatgatg gctgaaaatc
    23821 ggtgggtgat agcaagagag atctctattc atttcgatat tgaacacagc aaggcggtta
    23881 ataccctgac ttatattctg tcggaagtca cagaaataag ctgcgaagtt aagatgatcc
    23941 ctaataagct ggaagggcgg ggatgccagt gtcagcgact ggttaaagtg gttgatatcg
    24001 atgagcaaat ttacgcgcgt ctgcgcaata acagccggga taaattagtc ggcgtaagaa
    24061 agacgccgcg tattcctgcc gttccgctca cggaacttaa ccgcgagcag aagtggcaga
    24121 tgatgttgtc aaagagtatg cgtcgttaat gtcatttcgt tgataccacg cagcctggtt
    24181 gccggatgcg atgctggcgc atcttatccg gcctacggtt gctttacagc caggtcggat
    24241 aaggcgttta tgccgcatcc gacgatgtta ccgtttcggc gtcacatccg tcgtcccctg
    24301 caaacggggg cgattttcct ccatttgcct cagtggctgc gtttcatgta acgatgcatg
    24361 acagcgcccg acaagatcct gatactcttt ggtattcaac cgtttccagt gtaactcgtc
    24421 gtcactcacg ctgcgtacag cgcgggctgg cgtacccatc aacaactggc gtttctcgcc
    24481 gcgaaagccc gctttgacaa agctcatagc ggcaacaatg ctctcttcgc caatgaccgc
    24541 gccatccata atcacgctgt tcatcccgac cagtgcatcg cgaccaatca cacaaccatg
    24601 caggatcgct ccgtgcccga tatgaccgtt ttccccaacg atggtgtcag tgtcgcagta
    24661 gccatgcata atgcagccat cctgaatatt ggctcctgct tgcacgatca accgcccgta
    24721 gtcaccacgc agtgaggcga gtgggccgat gtagacaccg gctcccacaa tcacatcgcc
    24781 aatcaatact gcactgggat ggacaaacgc cgtcgggtga acaaccggaa ttaacccctc
    24841 aaaggcgtaa tagctcacgg ttgttaacgt cctttccaca ctggatcgcg cttctcggca
    24901 aacgccagcg gcccttcaat ggcatcctcc gaatgcagaa cagatggata gtgtttcaac
    24961 acgccgctgc gaatatagcg atacgcttct tctaccggca tttcactggt ggtgcggtag
    25021 atctctttca gcgccgcaat cgccagcgga gcgctgttaa ccagttgttg tgccagttcg
    25081 cgggcgttat ccatcagctc cgcctggcta accacgcggt tgactacccc ccaacgcagc
    25141 gcctcttctg cgcccattcg tctgccggtc atcaccattt cattgacgat ggcaggcggc
    25201 aggatcttcg gcagacgcag cacgccgccg ctgtcaggaa cgatgcccag tttggcttcc
    25261 ggtagggcga agctggcgtt atcggcacaa acaataaaat ctgccgccag cgccagttca
    25321 aagccgccgc caaaggcata gccgttcaca gctgcgatga ccggtttgtc gagattgaaa
    25381 atttcggtta atcccgcaaa acctcccggg ccaaagtcag catccggtgc ttcgccttct
    25441 gctgccgctt ttaaatccca gcccgcggaa aagaatttct ctccggcacc ggtaataatg
    25501 gcgacacgta attgcggatc gtcacggaaa tttagaaata cttcgcccat ttcaaagctg
    25561 gtttttgcat caatagcatt cgcttttgga cgatcaaggg taatttccag aattgatcca
    25621 ttgcgggtca gatgtaatga ttcactcatt ccttttctcc atttttgctt tttcagggac
    25681 gacaacatcc ctgcaaaaaa tgcatattgt tttagagcgt gattattagc tgacagggta
    25741 gttccctgcc gtttcattta tttcagattc tttctaatta ttttccccga gcaattacgt
    25801 ggcagatctt ttctgatctc cagataagag ggcactttaa atttcgccat attttgttcg
    25861 cagaagcgga aaaattcctc ttcgctcaat gtttcacctt cattcagcac cacaaatgct
    25921 ttgatggctt catcgcgaat cgaatcttta atacccacaa ccacgatatc ctgaattttg
    25981 ggatgggtgg cgatgatatt ttcgagctcc acgcaggaga cattctcgcc accgcgtttg
    26041 atcatattgc agcggcgatc gacgaaataa aaaaagccct cttcgtcgcg gtatccggta
    26101 tcgccggtat gtagccagcc atcggcttcc agcactttcg cagtggcttt cgggttgaga
    26161 aaatactctt tgaagatggt tttccctggt acgcctttaa tacagatttc accgatttca
    26221 cctgccggga gcgggcgatt gtgatcgtcg cggatctccg cttcgtagca aaaccccgcc
    26281 cgaccaatcg acggccagcg tcgtttatcg ccagggcgat cgccgataat gccaacaatg
    26341 gtttccgtca tcccataaga cgtcagcaag cgaacgccga agcgttcaca aaacgcatct
    26401 ttttcctgct ccgacaagtt gagataaaac atcacttccc gcaggcggtg ttgccgatcg
    26461 ttcgctgaag gcggctgcac catcaacgta cggatcatca tcggaataca ttcggtaatg
    26521 gtggcgcggt acttttgcac ctgtccccag aaggcacggg cgctgtattt ctcgaccagc
    26581 acaaaggtgg ccccggcaga aaacgccgcc atcgccgcag tacactggca atcgatatga
    26641 aacgcaggca ttaccgtcag gtagacgtca tcgtcacgca gtgcacactg ccaggcggag
    26701 taatatccag cgaagcgcag gttgtaatga gttatcacca cccctttcgg tcgggaggtg
    26761 gtgccggagg tgaagagaat ttccgccgta tcgtcagtcg atagcggcgg tgcatagcac
    26821 aaggtggcag gttgttgatt tttcagttga gtaaacgaac tcacgccatc atcagcggga
    26881 agtgccatat ctgtcaggca aatgtgccgc aactgagagg catcttcctg ctgaatctgt
    26941 tgatacatag gataaaattg cgcactggtc accagcaggc acgcctggct attttgcagg
    27001 atccacgtgc tttcttcgcg caacaggcgg gcgttaatcg gcaccataat cgcgccaatt
    27061 tttgccaacc cgaaccagca aaagataaat tccgggcagt tgtcgagatg tagagcaacc
    27121 ttgtcgcctt tgcgaatccc cagcgtataa aacaggtttg ccgtgcggtt aatctcctga
    27181 tttaactcaa gataactata ccggttaacg actccgctgc tggattcaca aatcagcgcc
    27241 gttttatgac cgtaaacgtc cgccagatcg tcccacattt gacgtagatg ttgtccgcca
    27301 atgatatcca ttgcacctct atccattttg ttcgtttgtt attgagcggg cgctagtcag
    27361 gcaagctgac tgacgccacg cgtttagtcc tcaactttgg ccagaccttt gctgaccaac
    27421 tcctgaatgt cgttttcgct gtagccgata tttttcaaaa tggcagccgt gtccatgcca
    27481 tgtgagggca ttccgcgcca gatttgtccg gggttatttt tgaatttcgg catgatattc
    27541 ggccctttgc aggtgcgacc atccatcgtt tgccactgag tgattgattc gcgagccaca
    27601 tactgtggat tgctttccag ttccggtacg gtcagtactt tggcacaggc gatattcagt
    27661 tcggcaaagc gttctttaac ttctgcgatg gtatgtgccg ccagccaggc atcgagtttc
    27721 tcttctacca gtgggccgta agggcattcg atacggtgga taagttgagt gcctgccggg
    27781 atttccggcg tgccgagcag atgtgcgagg ccaatatctt taaagcactc tgcaatttga
    27841 gtaatgccca ccagttccat cacgatgtag ccgtcggcgc atttatacag accgcaaccg
    27901 gcgtagtagg gatctttacc tttggtcatg cgcgggcaca tttcgccgcc gttgaagtaa
    27961 tccatcatga agtactggcc catacgcagc atcacttcat acatggcgat gtcgatgctt
    28021 tcgcctttgc cggtttcacg cactttatgc agtgctgcca gcgccgccgt ggtggcagtc
    28081 aggccagaaa agtaatcggc ggtatacggg aaggcaggca ttggctggtc aacatcaccg
    28141 ttctgaatca ggtaaccact gaaggcctgg gcgatagtgt tataggccgg aagattggtg
    28201 tactcctcgg tgccatactg accaaaaccg gacaggtgag cgataaccag ttttgggttg
    28261 tgctgccaca ggacttcatc ggtaatgcca cgacgggcaa aggccggacc tttactggct
    28321 tcgatgaaga tatcggtggt ttccattaat ttcagaaacg cttcacgacc ttcatctttg
    28381 aagatattta acgacagcgc gtgcaggttg cggcgggaaa gttgcgggta gttcggttga
    28441 acgcgaatgg tgtcggccca ggcgacgttt tcgatccaga taacttccgc gccccattct
    28501 gcgaacattt gtccggcaaa cggtccggcg atttcgatac cggagaagac aacgcgcaat
    28561 ccggacagcg gcccgaattt cggcatggtt agatgatcca ttattagctc ctgaaaaatt
    28621 tatgtagcgc atgactgccg gatgcggcgt aaacgcctta tccagcctac cgtcggcctg
    28681 ataagacgcg gcagcgtcgc atcaggcagc gcacggactt agcggtattg cttcagcacc
    28741 gcgcgaccca gcgtcaggat ctgcatttcg tcagatcccc cggaaacgcg gtctacacgc
    28801 aggtcacgcc agaagcggct gatgcggtgg ttgcccgcaa tcccgacacc gcccagcacc
    28861 tgcattgcgc tatccaccac ttcaaatgcc gcattggcgc agaagtattt gcacatcgct
    28921 gcatcgccag aggtgatggt gccgttgtct gctttccacg ctgcttcata cagcatgttt
    28981 ttcatggagt ttaatttgat cgccatgtgg gcgaattttt cctgaatcaa ctggaaacga
    29041 ccaatagcct cgccaaactg cacacgctgg ttggcgtagc gcgccgcatc ttcaaaggcg
    29101 cacatcgccg taccgtagtt ggtcagggct accaggaaac gttcatggtc gaactcttct
    29161 ttgacgcggt taaagccgtt accttcccga ccgaacatgt ctttctcgtc cagttccacg
    29221 tcgtcaaaag tgatttcaca gcagctatcc atacgcagac cgagcttttc aagtttggtc
    29281 actttgatgc ccggtttgct catatcaaca aaccattcgg tgtagacagg tttgtccgga
    29341 gaagccccgt cgcgcgccat caccacgatg tacggggtgt aggcgctgct ggtaataaaa
    29401 cacttactac cattaagata aatcttacca tttctacggg tataagtcgt tttcaggcta
    29461 cccacgtcgg agcccgcgcc cggttcggta attgctgagt tccacatctg cttaccggtg
    29521 ccgcggaaag ccatgatctt gtcgatctgc tcttgcgtac cttcacgcag gaaggtgtta
    29581 aacccgcccg gtaactgata cagcacgtaa gttggcgctc ccagacgacc cagttccatc
    29641 cacacggcgg cgagagtaac aaaccccgcg tccagaccac cgtgttcttc cgggatcagc
    29701 agactgtcga tacccatatc cgccagggct ttgacaaaac gttccgggta gacgctgtca
    29761 cggtcgcact cggcaaaata ggcctcccag ttttcgctgg ccatcagttc gcggataccg
    29821 gcgacaaaca gttcctgctc atcatttaaa ttaaaatcca tctttcaacc tcttgatatt
    29881 ttggggggtt aattaatctt tccagttctg tttcgcgtct ttaataaagg agagcgtcac
    29941 catgatgttg acgaagaaca gcgggcatcc tccggcgata atggcggttt gaatcggttt
    30001 caggccgccg agcgccagca gaacaatgcc gataatgcca accagaactg accaaccaat
    30061 gcgcaccagc agcgggggtt cttcgccgtc acgcacttcg cggcaagtgg acatcgccag
    30121 ggtataagag caggcgttaa ccagcgtaac ggtggcaata aagcagagga tgaagaagcc
    30181 ccacatggtg gcggtgctga gtggcagagc ggcccaggtt tcgatgatgg cgcgcgccac
    30241 accgtactgt tcgatcagat ttggaatgtt gataatgttt ttatctatca acagcagagt
    30301 gttactaccg agtacagtcc acaggatcca ggttgacgct gtcagtccca gcaccatgcc
    30361 gaagcacagt tcacgcacgg tacgaccacg ggagatgcgg gcgaggaaga tactcatctg
    30421 gatagcgtaa atcacccacc atgcccagta gaacacggtc cagccctgcg ggaagccgcc
    30481 tttagcgatg ggatcggtat agaacaacat gcgcggcaga tacatcagca acatccccac
    30541 cgaatcggtg aagtagttca tgatgaagct ggcaccgctg acgatgaaca cccaacccag
    30601 catcaggaag ctcaggtaac tacgcacgtc actggcgata cgtacccctt tttgcagacc
    30661 gcaagcgacg caaatggcgt tgaggataat ccagcaggta atgatgatag cgtccagttg
    30721 cagggtatgc ggaatgccaa acaaccattg catacactcg gtcaccagcg gcgtggcaag
    30781 gcccagactg gtacccatcg cgaagatcaa ggcgacgaga tagaagttgt cgacgatagt
    30841 gccgaacaac cctttggcgt gtttttcacc taccagcggc accagggtgg agctggggcg
    30901 aatcacttcc attttgcgga caaagaagaa gtaagcgaag gcgactgaaa ggaagctgta
    30961 agtggcccac ggcagaggtc cccagtggaa caagctgtaa gccagcccca actctttcgc
    31021 gcctgttgag ttcggttcta agccaaacgg cggggtggag atgtagtagt agatctcaat
    31081 cgatccccag aacagtacgg cagcagacgt acaggaggcg aacatcataa agatccaact
    31141 ggcggtacta aattctggtg gttcgttacc taaacgcttt ttggcatacg ggccaaacac
    31201 cagccagaac cagccgaaaa gcatcaccac catataccat tcaaatgccc atccccatac
    31261 attggtgacg taactgaata cagcattaat aacgacattc gccgcatcca gatctctgac
    31321 tgtaagccaa caaagtatgc cgacgattat taacggcgga aagaaaacct tcggttctat
    31381 tcccgttttt ctcttttcat tcttcatgag ttaattccac tgtgaaaacg aatatttatt
    31441 ttgcgttccc gtttgtttta tttttgttaa catttaatat aattattatt aacctcgtgg
    31501 acgcgttaat ggctaactca taatgggtat tcaataagct gcattctgtg attgttgtca
    31561 catttttgtt tcgggtgaat agagggcatt ttttcgttaa ttttgattat taatcagttt
    31621 gttatgttct gttatgagta aaaaaatagc atctgacttt caatattggt gatccataaa
    31681 acaatattga aaatttcttt tttctacgcc atgttttcaa tattggtgag gagcttaaca
    31741 atattgaaag ttggatttat ctgcgtgtga cattttcaat attggtgatt aaagttttat
    31801 ttccaaatta aagggcgtga tatctgtaat taacaccacc gatatgaacg acgtttcctt
    31861 catgatttct ggagatgcaa tgaagattat tacttgctat aagtgcgtgc ctgatgaaca
    31921 ggatattgcg gtcaataatg ctgatggttc gttagacttc agcaaagccg atgccaaaat
    31981 aagccaatac gatctcaacg ctattgaagc ggcttgccag ctaaagcaac aggcagcaga
    32041 ggcgcaggtg acggccttaa gtgtgggcgg taaagccctg accaacgcca aagggcgtaa
    32101 agatgtgcta tcgcgcggtc cggatgaact gattgttgtg attgatgacc agttcgagca
    32161 ggcactgccg caacaaacgg cgagcgtact tgctgcagcc gcccagaaag caggctttga
    32221 tttgatcctc tgtggcgatg gttcttccga cctttatgcc cagcaggttg gtttgctggt
    32281 aggcgaaatc cttaatattc cggcagttaa cggcgtcagc aaaattatct ccctgacggc
    32341 agataccctc accgttgagc gcgaactgga agatgaaaca gaaaccttaa gcattccgct
    32401 gcctgcggtt gttgctgttt ccactgatat caactcccca caaattcctt cgatgaaagc
    32461 cattctcggc gcggcgaaaa agccagtcca ggtatggtcg gcggcggata tcggttttaa
    32521 cgcagaggca gcctggtcag aacaacaggt tgccgcgccg aaacagcgcg agcgtcagcg
    32581 catcgtgatt gaaggcgacg gcgaagaaca gatcgccgca tttgctgaaa atcttcgcaa
    32641 agtcatttaa ttacagggga tgctatgaac acgttttctc aagtctgggt attcagcgat
    32701 accccttctc gtctgccgga actgatgaac ggtgcgcagg ctttagctaa tcaaatcaac
    32761 acgttcgtcc tcaatgatgc cgacggcgca caggcaatcc agctcggcgc taatcatgtc
    32821 tggaaattaa gcggcaaacc aaacgatcgg atgatcgaag attacgccgg tgtcatggct
    32881 gacactattc gccagcacgg cgcagacggt ctggtgctgt tgccaaacac ccgtcgcggc
    32941 aaattactgg cggcaaaact gggttatcgc cttaacgcgg cggtgtctaa cgatgccagc
    33001 accgtcagcg tacaggacgg taaagcgaca gtgaaacaca tggtttacgg cggtctggcg
    33061 attggcgaag aacgcattgc cacgccgtat gcggtactga ccatcagcag cggcacgttc
    33121 gatgcggctc agccagacgc gtcacgcact ggcgaaacgc acaccgtgga gtggcaggat
    33181 ccggctgtgg cgattacccg cacggcaacc caggcgcgtc agagcaacag cgtcgatctc
    33241 gacaaagccc gtctggtggt cagcgttggt cgcggtattg gcagcaaaga gaacattgcg
    33301 ctggcagaac agctttgcaa ggcgataggt gcggagttgg cctgttctcg tccggtggcg
    33361 gaaaacgaaa aatggatgga gcacgaacgc tatgtcggta tctccaacct gatgctgaaa
    33421 cctgaactgt acctggcggt ggggatctcc gggcagatcc agcacatggt tggcgctaat
    33481 gcgtcgcaaa ccattttcgc catcaacaaa gataaaaatg cgccgatctt ccagtacgcg
    33541 gattacggca ttgttggcga cgccgtgaag atccttccgg cactgaccgc agctttagcg
    33601 cgttgatcca ctctggcagg gctgcatttt ggccctgccg ctgacaggga gctcttatgt
    33661 ccgaagatat ctttgacgcc atcatcgtcg gtgcagggct tgccggttcg gttgccgcac
    33721 tggtgctcgc ccgcgaaggt gcgcaagtgt tagttatcga gcgaggcaat tccgcaggtg
    33781 ccaagaacgt caccggcggg cgtctctatg cccacagtct ggaacacatt attcctggtt
    33841 tcgctgactc cgcccccgta gaacgcctga tcacccatga aaaacttgcg tttatgacgg
    33901 aaaagtcagc gatgactatg gactactgca atggcgacga aacctcgcca tcccagcgtt
    33961 cttactccgt tttgcgcagt aaatttgatg cctggctgat ggagcaggcc gaagaagcgg
    34021 gcgcgcagtt aattaccggg atccgcgtcg ataacctcgt acagcgcgat ggcaaagtcg
    34081 tcggtgtaga agccgatggc gatgtgattg aagcgaaaac ggtgatcctt gctgatgggg
    34141 tgaattccat ccttgccgaa aagctgggga tggcaaaacg cgttaaaccg acggatgtgg
    34201 cggttggcgt gaaggaactg atcgagttac cgaagtcggt tattgaagac cgttttcagt
    34261 tgcagggtaa tcagggcgcg gcttgcctgt ttgcgggatc acccaccgat ggcctgatgg
    34321 gcggtggctt cctttatacc aatgaaaata ccctgtcgct ggggctggtt tgtggtctcc
    34381 atcatctgca tgacgcaaaa aaatcggtgc cgcaaatgct ggaagatttc aaacagcatc
    34441 cggccgttgc accgctgatc gcgggcggca agctggtgga atattccgct cacgtagtgc
    34501 cggaagcagg catcaacatg ctgccggagt tggtcggtga cggcgtattg attgctggtg
    34561 atgccgccgg aatgtgtatg aacctcggtt ttaccattcg cggtatggat ctggcgattg
    34621 ccgccgggga agcagcagca aaaactgtgc tttcagcgat gaaaagcgac gatttcagta
    34681 agcaaaaact cgcggaatat cgtcagcatc ttgagagtgg cccgctgcgc gatatgcgca
    34741 tgtaccagaa actaccggcg ttccttgata acccacgcat gtttagcggc tacccggaac
    34801 tggcggtggg cgtggcgcgt gacctgttca ccattgacgg cagtgcgccg gaactgatgc
    34861 gcaagaaaat cctccgccac ggcaagaaag tgggcttcat caatctgatc aaggatggca
    34921 tgaaaggagt gaccgtttta tgacttctcc cgtcaatgtg gacgtcaaac tgggcgtcaa
    34981 taaattcaat gtcgatgaag agcatccgca cattgttgtg aaggccgatg cagataaaca
    35041 ggccctggag ctgctggtga aagcgtgccc cgcaggtctg tacaagaagc aggatgacgg
    35101 cagcgtgcgt ttcgattacg ccggatgtct ggagtgtggc acctgccgca ttctggggct
    35161 ggggtcggcg ctggaacagt gggaataccc gcgcggcacc tttggtgtgg agttccgtta
    35221 cggctgatgt tggtttgata cgtaacgccg tactgactct cattgcaaaa aaacaggaat
    35281 aacccatgca accgtccaga aactttgacg atcttaaatt ctcctctatt caccgccgca
    35341 ttttgctgtg gggaagcggt ggtccgtttc tggatggtta tgtactggta atgattggcg
    35401 tggcgctgga gcaactgact ccggcgctga aactggacgc tgactggatt ggcttgctgg
    35461 gcgcgggaac gctcgccggg ctgttcgttg gcacatcgct gtttggctat atctccgata
    35521 aagtcggacg gcgcaaaatg ttcctcatcg atatcatcgc catcggcgtg atatcggtgg
    35581 cgacgatgtt tgtctcatcc cccgtcgaac tgttggtgat gcgggtactt atcggcattg
    35641 ttatcggtgc ggattatcct atcgccacct caatgatcac cgagttctcc agtacccgtc
    35701 agcgggcgtt ttccatcagc tttatcgccg cgatgtggta tgtcggcgcg acctgcgccg
    35761 atttggtcgg ctattggctt tatgatgtgg aaggcggctg gcgctggatg ctgggtagcg
    35821 cagcgattcc ctgtttgttg attttgattg gtcgattcga actgcctgaa tctccccgct
    35881 ggttgttacg caaagggcga gtaaaagagt gcgaagagat gatgataaaa ctgtttggcg
    35941 aaccggtggc tttcgaggaa gagcagccgc agcaaacccg ttttcgcgat ctgtttaatc
    36001 gccgccattt tccttttgtt ctgtttgttg ccgctatctg gacctgccag gtgatcccca
    36061 tgttcgccat ttacaccttt ggcccgcaaa tcgttggttt gttgggattg ggggttggca
    36121 aaaacgcggc actggggaac gtggtgatta gcctgttctt tatgctcggc tgtattccgc
    36181 cgatgctgtg gctaaacact gccggacggc gtccattgtt gattggcagc tttgccatga
    36241 tgacgctggc gctggcggtt ttggggctga tcccggatat ggggatctgg ctggtagtga
    36301 tggcctttgc ggtgtatgcc tttttctctg gcgggccggg taatttgcag tggctctatc
    36361 ctaatgaact cttcccgacg gatatccgcg cctctgccgt gggcgtgatt atgtccttaa
    36421 gccgtattgg cactatcgtt tcgacctggg cactgccgat ctttatcaat aattacggta
    36481 tcagtaacac gatgctaatg ggggcgggta tctcgctatt tggcttgttg atttccgtag
    36541 cgtttgcccc ggagactcga gggatgtcac tggcgcagac cagcaatatg acgatccgcg
    36601 ggcagagaat ggggtaattt gttcagattt ctctcttttc tgaatcaata ttattgacta
    36661 taagctgcgt gaatatatga ctacactttg tgggaaaaca aaggcgtaat cacgcgggct
    36721 acctatgatt cttataattt atgcgcatcc atatccgcat cattcccatg cgaataaacg
    36781 gatgcttgaa caggcaagga cgctggaagg cgtcgaaatt cgctctcttt atcaactcta
    36841 tcctgacttc aatatcgaca ttgccgccga acaggaggcg ctgtctcgtg ccgatctgat
    36901 cgtctggcag cacccaatgc agtggtacag cattccaccg cttcttaaac tttggatcga
    36961 taaagttttc tcgcacggct gggcttacgg tcacggcggc acggcgctgc acggcaaaca
    37021 tttgctgtgg gcggtgacga ccggcggtgg ggaaagccat tttgagattg gtgcgcatcc
    37081 gggctttgat gtgctgtcgc agccgctaca ggcgacggca atctactgcg ggctgaactg
    37141 gctgccaccg tttgccatgc actgcacctt tatttgtgac gacgaaaccc tcgaagggca
    37201 ggcgcggcac tataagcaac gtctgctgga atggcaggag gcacatcatg gatagccata
    37261 cgcttattca ggcgctgatt tatctcggtt cggcggcgct gattgttccc attgcggtac
    37321 gtctcggtct gggatcggtt cttggctacc tgatcgccgg ctgcattatt ggcccgtggg
    37381 ggctgcgact ggtgaccgat gccgaatcta ttctgcactt tgcagagatt ggcgtggtgc
    37441 tgatgctgtt tattatcggc ctcgaactcg atccacaaag gctgtggaag ctgcgtgcgg
    37501 cagtgttcgg cggtggcgca ttgcagatgg tgatttgcgg cggcctgctg gggctgtttt
    37561 gtatgttgct tgggctgcgc tggcaggtcg cggaactgat cggcatgacg ctggcgctct
    37621 cctctacggc gattgccatg caggcgatga atgaacgcaa tctgatggtg acgcaaatgg
    37681 gccgcagtgc ctttgcggtg ctgctgttcc aggatatcgc agcgatcccg ctggtggcga
    37741 tgatcccgct actggcagcg agcagtgcct cgacgacgat gggtgcattt gttctctcgg
    37801 cgttaaaagt ggcgggcgcg ctggtgctgg tggtattgct ggggcgctat gtcacgcgtc
    37861 cggcactgcg ttttgttgcc cgctctggct tgcgggaagt gtttagtgcc gtggcgttat
    37921 tcctcgtgtt tggctttggt ttgctgctgg aagaggtcgg cttgtcgatg gcgatgggcg
    37981 cgtttctggc gggcgtactg ctggcaagct cggaataccg tcatgcgctg gaaagcgata
    38041 tcgaaccatt taaaggtttg ctgctggggc tgtttttcat cggcgttggc atgtcgatag
    38101 actttggcac gctgcttgaa aatccgttgc gcattgtcat tttgctgctc ggtttcctca
    38161 tcatcaaaat cgccatgctg tggctgattg cccggccgtt gcaggtgcca aataaacagc
    38221 gtcgctggtt tgcggtgcta ttagggcagg gtagtgagtt tgcctttgta gtatttggtg
    38281 cggcgcagat ggcgaatgtg ctggagccag agtgggcgaa atcgctgacc ctggcggtgg
    38341 cactgtcgat ggcggcaacg ccgattctgc tggtgatcct caatcgcctt gagcaatctt
    38401 ctactgaaga ggcgcgtgaa gccgatgaga tcgacgaaga acagccgcgg gtaatcattg
    38461 ccggattcgg tcgttttggg cagataaccg gacgtttact gctctccagc ggggtgaaaa
    38521 tggtggtgct cgatcacgac ccggaccata tcgaaacgct gcgtaaattc ggtatgaaag
    38581 tgttttatgg cgatgccacg cggatggatt tactggaatc tgccggagcg gcgaaagcgg
    38641 aagtgctgat taacgccatc gacgatccgc aaaccaacct gcaactgacg gagatggtga
    38701 aagaacattt cccacatttg cagattattg cccgcgcccg cgatgtcgac cactacattc
    38761 gtttgcgtca ggcaggcgtt gaaaagccgg agcgtgaaac cttcgaaggt gcgctgaaaa
    38821 ccgggcgtct ggcactggaa agtttaggtc tggggccgta tgaagcgcga gaacgtgccg
    38881 atgtgttccg ccgctttaat attcagatgg tggaagagat ggcaatggtt gagaacgaca
    38941 ccaaagcccg tgcggcggtc tataaacgca ccagcgcgat gttaagtgag atcattaccg
    39001 aagaccgtga acatctgtca ttaattcaac gacatggctg gcagggaacc gaagaaggga
    39061 aacatactgg caatatggcg gatgaaccgg aaacgaagcc ctcatcctaa taaagagtga
    39121 cgtaaatcac gctttacagc taactgtttg tttttgtttc attgtaatgc ggcgagtcca
    39181 gggagagagc gtggactcgc cagcagaata taaaattttc ctcaacatca tcctcgcacc
    39241 agtcgacgac ggtttacgct ttacgtatag tggcgacaat tttttttatc gggaaatctc
    39301 aatgatcagt ctgattgcgg cgttagcggt agatcgcgtt atcggcatgg aaaacgccat
    39361 gccgtggaac ctgcctgccg atctcgcctg gtttaaacgc aacactttaa ataaacccgt
    39421 gattatgggc cgccatacct gggaatccat cggtcgtccg ttgccaggac gcaaaaatat
    39481 tatcctcagc agtcaaccgg gtacggacga tcgcgtaacg tgggtgaagt cggtggatga
    39541 agccattgcg gcgtgtggtg acgtaccaga aatcatggtg attggcggcg gtcgcgttta
    39601 tgagcagttc ctgccgaaag cgcaaaaact gtatctgacg catatcgacg cggaagtgga
    39661 aggcgacacc catttcccgg attacgagcc ggatgactgg gaatcggtat tcagtgaatt
    39721 ccacgacgcc gatgcgcaga actctcacag ctattgcttt gagattctgg agcggcggta
    39781 atttcgtata ttagaattcc ggcatcactg atacgtgcag atatccgtgt atactagacg
    39841 tataaattgt acaggagcac gagatcatga ctgcaaaacg taccacacaa agtgtgaccg
    39901 tcaccgtcga ccgtgagtta gtcaatcgcg ctcgtgatgc aggcttaaat atgagcgcca
    39961 cccttacggt tgcgctcaat gctgaactta aaaaacatgc agcaacacgt tggcgtgaag
    40021 agaacgcaga agctatcgct gcgttaaatc aattggctga tgaaaccgga tgtttctctg
    40081 atgagtaccg gagcttctag cgatgcaatt tacggtatac cgcagtcgcg gcaggaacgc
    40141 cgcgtttccc tttgttattg atgttaccag cgacattatt ggtgagatta atcgccgtat
    40201 cgttattcca ttaacaccta ttgaacgatt tagccgtatt cgcccaccag aacggcttaa
    40261 cccaatcctt ttactggtag acggcaaaga atatgtgctc atgacgcatg agactgcaac
    40321 tgttccagtt aacgcgctgg gaacgaaatt ttgcgatgcc agcgcgcacc gaacgttgat
    40381 taaaggagca ttagatttta tgctcgacgg gatttaagac gctaccgcct ctccctcact
    40441 caaatcctta tgccggttcg acggctggac aaaatactgt ttatcttccc agcgcaggca
    40501 ggttaatgta ccaccccagc agcagccggt atccagcgcg taaatacctt ccggcgtacc
    40561 tttgccttcc agcgatgccc agtgaccaaa ggcgatgttg tactcttcag cgacggggcc
    40621 aggaatcgca aaccacggtt tcagtggggc aggggcctct tccggcgatt ctttgctgta
    40681 catatccagt tgaccgttcg ggaagcaaaa acgcatacgg gtaaaggcgt tggtgataaa
    40741 acgcagtctt cccagccccc gcaattccgg tgaccagtta tttggcatat cgccgtacat
    40801 ggcatcaagg aagaagggat aggagtcact cgacagcacc gcttctacat cgcgcgcgca
    40861 ctctttggcg gtctgcagat cccactgcgg cgtgatccca gcgtgggcca tcaccaactt
    40921 tttctcttcg tcgatttgta gcaaaggctg gcgacgcagc cagttaagca gctcgtcggc
    40981 atccggcgct tccagcagcg gtgtcaggcg atctttcggt ttattgcggc tgatcccggc
    41041 aaataccgcc agcagatgca gatcgtgatt acccagcacc agacgtacgc tgtcgcctaa
    41101 ggatttcaca tagcgcagaa catccagcga acccgggccg cgcgcgacca gatcgcccgt
    41161 cagccagagg gtatctttcc caggggtaaa ttctacttta tgcagcaatg cgatcagttc
    41221 atcgtaacaa ccatgaacgt cgccaataag gtatgtcgcc atattctttt aatgaatgag
    41281 tgtgggaacg gcgagtcgga atacgggaat gtcgatgctg aaagggacgc cattttcatc
    41341 gatcatttcg tagtgaccct gcatggtgcc cagcggggtt tcaatgattg caccgctggt
    41401 gtactggtac tcttcgccag gcgcgataag tggctggacg ccaaccactc cttcgccctg
    41461 gacttcggtt tcacggccat tgccattggt gatcagccag taacgcccca acaactgcac
    41521 tggcgctcgc cccagattgc gtatggttac ggtataagca aaaacgtaac gttcattatc
    41581 aggtgaagat tgtgcctcaa tgtagacgct ttgaacctga atacacactc ggggcgaatt
    41641 gatcatcgtt aactctcctg caaaggcgcg ttctccgcca gatagttcgc catctggcaa
    41701 tattgcgcga cagagatatt ttccgctcgc atcgccgggt cgatccccat tcccgttaac
    41761 acctcgacgc taaacaggtt gccgaggctg ttacgaatgg ttttacgacg ctggttaaag
    41821 gcttcggtgg tgatgcggct caacacgcga acatctttaa ccgggtgagg catcgttgca
    41881 tgaggaacca ggcgcacgac ggcggaatcc actttgggtg gtggtgtaaa ggctgacggc
    41941 ggtacttcca gcaccgggat cacgttgcaa tagtattgcg ccatgacgct taatcgacca
    42001 tacgccttgc tgttcggtcc tgcaaccaga cgattcacca cctctttttg caacataaag
    42061 tgcatgtcgg caatggcatc agtatagcta aacagatgga acatcaacgg cgtggagatg
    42121 ttataaggca ggttgccgaa aacacgcagc ggctggccca ttttcgcggc cagttcacca
    42181 aagttaaagg tcatcgcatc ctgctgataa atcgtcagtt tcgggcctaa gaatggatgc
    42241 gtttgcagac gtgccgccag atcgcggtca agttcgatga ccgtcagctg gtccagacgt
    42301 tcgccgaccg gttcggtcaa tgccgccaga ccggggccga tttcgaccat cgcctggccc
    42361 ttctgcgggt taatggcaga aacaatgctg tcgatcacga actgatcgtt gagaaagttt
    42421 tgcccgaagc gtttacgggc taagtggccc tggtggactc gattattcat tgggtgttaa
    42481 caatcatttt gatggcgaga ttaagcgccg taataaaact gccgacatcg gctttaccac
    42541 gtcccgccag ttcaagcgcg gtgccgtggt ccactgatgt gcgaataaag ggcaggccca
    42601 gcgtaatgtt cacaccgcgc ccgaagccct ggtattttag cacgggaaga ccctgatcgt
    42661 ggtacatcgc cagcacggcg tcggcgttat cgagatattt cggctgaaac agggtatcgg
    42721 caggcagcgg cccgttgagt ttcatcccct gttcccgcag ctcgtcgagt accggaataa
    42781 tggtgtctat ctcttccgta cccatatgac cgccttcgcc cgcgtgcgga ttcagcccgc
    42841 agaccagaat gcgcggttcg gcaataccaa atttggtgcg caaatcgtga tgcaaaatag
    42901 caatcacttc gtgcaaaagg gcaggggtga tagcatctgc gatatcgcgc agcggtaaat
    42961 gcgtcgttgc cagcgccacg cgaagttctt cggtcgccag catcatcacc acttttttcg
    43021 cctgcgaacg ctcttcgaaa aactcggtat gaccggtaaa cggaatgcct gcgtcgttaa
    43081 tgacgccttt atgcacgggg cctgtgatca gcgcagcaaa ttcaccgttc agacagccat
    43141 cacacgctcg cgccagcgtt tccaccacat agtgcccatt ttcaaccgct aacagcccca
    43201 cagtgacaga ttcacgtagc gcgacaggaa gtagcgttaa tgtgcccgca gtttgcggtt
    43261 gtgcagggga gttgggggaa taagggcgga gggtgagcgg caaaccgagc atcgctgccc
    43321 ggtcggtaag gagagtggca tcggcacaaa caaccagttc gaccggccac tcacgctgtg
    43381 caagctggac aattaagtcc gggccaatcc cggcgggctc gccgggagtg atcacaacac
    43441 gttgggtttt aaccattagt tgctcaggat tttaacgtag gcgctggcac gttgttcctg
    43501 catccagctt gctgcttctt ccgagaactt acggttcatc agcatgcggt atgcacgatc
    43561 tttctgtgcc gcgtcggttt tatcgacatt acgggtatcc agcagttcga ttaaatgcca
    43621 gccgaatgaa gagtgaaccg gtgcactcat ttgacctttg ttcaggcggg tcagggcgtc
    43681 acggaaggcc ggatcgaaaa tatctggtgt agcccagccg agatcaccgc cctggttagc
    43741 agagcctgga tcctgagaga actctttcgc tgcggcagca aaagtcgttt taccactctt
    43801 gatatcggca gcaatctgtt ccagtttcac gcgggcctgt tcgtcagtca tgatcggcga
    43861 cggtttcagc agaatatggc gagcatgaac ttcggtcacc gagatatttt tgctttcgcc
    43921 gcgcaggtcg ttaactttca gaatatggaa gccaacgccg gaacgaatcg ggccaacaat
    43981 gtcgcctttc ttcgcggtgc ttaatgcctg ggcgaagatc cccggcaact cctgaatacg
    44041 gccccagccc atctggccgc cgttcagcgc ctgctggtcg gcagaatgag caatcgccag
    44101 cttaccgaaa tcagcgccgt tacgcgcctg atcgacaatc gcgcgcgcct ggctttccgc
    44161 ttcgttcacc tgatcagagg tcgggttttc cggcagcggg atcaggatgt ggctcaggtt
    44221 cagctcagtg ctggcgtcgt tttggttacc cacttgctgc gccagggatt cgacttcctg
    44281 cggcaggatg gtgatgcgac gacgcacctc gttgttacgc acttcagaga taatcatctc
    44341 tttgcggatc tggttacgat aggtgttgta gttcagacca tcgtaagcca gacggctgcg
    44401 catctgatcc agcgtcatgt tgttctgttt cgcgatgtta gcaatcgcct gatccagctg
    44461 ctcatcggag attttcactc ccattttctg ccccatctgc aggataattt gatccatgat
    44521 caaacgttcc atgatttggt ggcgcagcgt cgcgtcatca ggaagttgtt gccttgcctg
    44581 agcagcgttc agttttaccg actgcattaa tccatcaacg tcgctttcca gcacgacgcc
    44641 gttattgacg acggctgcga ctttatcgac tacctggggg gcagcgaaac tggtattcgc
    44701 gatcatggcg ataccgagaa gcagcgtttt ccagttcttc atactttttc catttcaatt
    44761 aaccgcactg cggattacgt ggtaaatcaa caaatcacaa agtgttttga tacggcagaa
    44821 tgttcgaacg cagcatctct tgcgtaccca gaccgtagtt ggagctcagg ccgcgaagtt
    44881 cgatgttaaa gccgattgcg ttgtcatata ccgcatgttg tttatcgtta tcccaaccgt
    44941 tcagcttccg ctcgtaaccg acgcgaatcg catagcagca ggagctgtat tgcacaccta
    45001 acatagagtc ggcttgcttg ttcgcattgg tgtcgtagta gtaggcccca acaatggacc
    45061 aacgatcggc aattggccag ctggcgacgg cacctacctg cgaaatacca ttcttatatt
    45121 gctcagcagt ggaatagtac ttaggcagcg tagcctgaat atattccggg ctggcgtaac
    45181 ggtaattcag ctgtaccaga cggtcttcat cacgacggta ttcaatgctg gagttactgg
    45241 tcgctacgtt atccagacgt gtatcgtact gaatcccgcc acgcaatccc caacgatcgg
    45301 agatgcgcca gtaagtatcg cctgcccata ccagtgagcc cgttttgtcg tcattctccc
    45361 atgttatgtt gtcatcgcca gtgcgagact ccgtgaaata gtagatttga ccaacggaaa
    45421 tattaaaacg ttcaacggca gcatcatcat atatgcgaga tgtgacaccg gtcgtcacct
    45481 ggttagcgga ggcaatacgg tcaagaccgc cgtaagtccg gtcccggaac aggccggagt
    45541 agtcagattg cagcagcgaa gagtcgtagt tatagatttt gctctgatcg cgatatggca
    45601 catacagata ctgcgcgcgt ggttccagtg tctgggtata gcccggcgcc agcatttcca
    45661 tatcacgctc gaagaccatt ctaccgtcta ccttgaactg cggcattaca cggttagcgg
    45721 actctgccag cctggtcgtg tttctggagt tataccagtc aagattggtt tgctgataat
    45781 gggttgccag caacttcgct tcagtattga tgctgcccca gttattagag agcggcagat
    45841 tgatggtcgg ttccaggtga acacgggttg cttcaggcat gtcgtctctg gtgttaacaa
    45901 agtgcactgc ctgaccgtaa atacgcgtat caaacggacc aacatcattc tggtagtagt
    45961 taacgtctaa ctgcggctct gccgagtagc tactggtgtt ctgctcgctg aagacctgga
    46021 actgcttggt tgaaacggtg gcattgaagt tttgcaccgc atagccaacg ctgaattttt
    46081 gcgttgcgta gccgtcggta ctggaaccgt atttgttatc gaagtcattg aagtagctag
    46141 gatcgctgac tttggtgtag tcgacgttga aacgccacac ctgatccatg accccggagt
    46201 ggttccagta gaacaaccaa cgacgtgaac tgtcatcgtt cgggtgttca tctttataga
    46261 ctttatctga aggcagatag tccagttcca tcaagccagc gcccgcctgg gagaggtagc
    46321 ggaattcgtt ctcccacatg atgttgccac gacgatgcat ataatgcggc gtgatggtgg
    46381 catccatatt tggcgcgatg ttccagtaat atggcaggta gaactcaaag tagttggtgg
    46441 tggtgtactt ggcgttcggg atcaagaaac cagagcgacg tttgtcaccc accggcaact
    46501 gcaaatacgg gctataaaag atcggtaccg gacccacctt aaagcgggcg ttccagatct
    46561 ccgcaacttg ttcttcgcgg tcatgaataa tttcgctacc taccacgctc caggtgtcag
    46621 aacccggcag acaggaggta aagctaccgt tatccagaat ggtatagcgg ttttcgccac
    46681 gttgtttcat caggtccgct ttaccgcgcc cctggcgacc caccatctgg taatcacctt
    46741 cccagacgtt ggtatctttg gtgttcagat tcgcccagcc tttcggccct ttgaggatca
    46801 cctggttatc gtcgtaatgg acattaccga gcgcatcaac ggtacgtacc ggctccggtt
    46861 gtcctggtgc ctctttttga tggagctgca cttcgtcggc ctgcagacgg ctgttaccct
    46921 gcatgatatc cacgctgcca gtaaacacgg cgtcatccgg gtagtcccct ttcgcgtggt
    46981 cagcattgat agtcacgggt aagtcattgg tatcgccctg taccagagga cggtcatagc
    47041 ttggcacgcc caacatgcac tgtgaggcga ggtcggctgc cagtccctgt tgactataaa
    47101 gggcggtggc aatcatggtg gccaggagag tggggatacg ttttttcata cgttgatttt
    47161 attgttccat catcggtaac gttgcgcgtg acaaacggtc agagactaac gtactcgtca
    47221 tctctacgct agtgttaatc ctgtccgaat agcgtcagtg gtgttaggca cggcattgaa
    47281 tgacaggtat gataatgcaa attataggcg atgtcccaca attgaccgca gccggaaaac
    47341 ggtaaaagca cctttatatt gtgggagata gccctgatat ccgtgtgtcg atttggggaa
    47401 tatatgcagt attggggaaa aatcattggc gtggccgtgg ccttactgat gggcggcggc
    47461 ttttggggcg tagtgttagg cctgttaatt ggccatatgt ttgataaagc ccgtagccgt
    47521 aaaatggcgt ggttcgccaa ccagcgtgag cgtcaggcgc tgttttttgc caccactttt
    47581 gaagtgatgg ggcatttaac caaatccaaa ggtcgcgtca cggaggctga tattcatatc
    47641 gccagccagt tgatggaccg aatgaacctt catggcgttt cccgtactgc ggcgcaaaat
    47701 gcgttccggg tgggaaaatc agacaattac ccgctgcgcg aaaagatgcg ccagtttcgc
    47761 agtgtctgct ttggtcgttt tgacttaatt cgtatgtttc tggagatcca gattcaggcg
    47821 gcgtttgctg atggttcact gcacccgaat gaacgggcgg tgctgtatgt cattgctgaa
    47881 gaattaggga tctcccgcgc tcagtttgac cagtttttgc gcatgatgca gggcggtgca
    47941 cagtttggcg gcggttatca gcagcaatct ggcggtggta actggcagca agcgcagcgt
    48001 ggcccgacgc tggaagatgc ctgtaatgtg ctgggcgtga agccgacgga tgatgcgacc
    48061 accatcaaac gtgcctaccg taagctgatg agtgaacacc atcccgataa gttggtggcg
    48121 aaaggtttgc cgccagagat gatggagatg gcgaagcaga aagcgcagga aattcagcag
    48181 gcctatgagc tgataaagca gcagaaaggg tttaaataag tttcaacgtg taggcctgat
    48241 aagacgcggt aggccggata agacgcgcca gcgtcgcatc cggcgagcag catcagacaa
    48301 tcggcgcaca attgccggat gtggcgtgaa cgccttctcc ggcctaccga acttcgccag
    48361 cgtgactatt aaaaatccgc tggtgcttta aacgtcatac tattgccata cgccggatgg
    48421 gtaatcgtca gcatctctgc atgtagcaac aaacgcggtg ccattgctct cgcttctggt
    48481 gatgcataaa aacgatcgcc gagaatcgga tgacccagcg ccagcatatg cacacgcagc
    48541 tgatgcgaac gcccggtaat cggttttaac accactcttg ccgtgttatc cgccgcatat
    48601 tccaccactt catactccgt ctgcgcaggt ttacctgttt cgtaacagac tttctgtttc
    48661 gggcggtttg gccagtcgca aatcagcggc agatccacca gaccttccgc aggggatgga
    48721 tgcccccaga cgcgggccac atactgtttt ttcggctcgc gctcgcggaa ctggcgtttt
    48781 aactcccgct ccgcggcttt ggtcagcgcc actacaatca cgccgctggt agccatatcc
    48841 agacgatgca ccgattctgc ctgcggataa tcacgctgaa tgcgcgtcat cacgctgtct
    48901 ttgtgctctt ccagacgccc cggcacagac aacaaaccgc tcggcttgtt gaccaccata
    48961 atgtggtcat cctgatacag gataaccaac cagggttcct gcggtggatt gtagttttcc
    49021 atccccattt tcggctccgt tactgatgcg tcacaacgat caaacgcagg gcatccagac
    49081 gccagcctgc ctgatccagg ctttccatta cctgctgacg gttgctctca atggcggtca
    49141 gttcgtcgtc acgaatgttc gggttaactg cacgcagcgc ttccagacga gacagctcgg
    49201 cagacagttt ttcgtcggct tcgttacgcg ctgcatcaat cagtgcacgg gcagatttct
    49261 cgatctgcgc ttcacccagc tgaaggatag cgtgaacatc ctgctgcacg gcgttaacca
    49321 gcttgctgcc ggtgtgacgg ttaaccgcgt taagctgacg gttaaaggtt tcaaattcta
    49381 cctgcgccgc caggttgttg ccgtttttat ccagcagcat acgtaccggc gtcggtggca
    49441 ggaagcggtt gagctgcaac tgcttcggag cctgggcttc aacgacataa atcagttcca
    49501 ccaacagcgt acctaccggc aacgctttgt ttttcaacag tgaaatcgtg ctgctaccgg
    49561 tatcgccaga aaggatcaga tccagaccgt tgcggatcag cggatgctcc caggtaataa
    49621 actgtgcatc ttcacgcgcc agcgccactt cacggtcgaa ggtgatggtg atgccatctt
    49681 ccgacaggcc agggaagtcc ggcaccagca tatgatcgga cggcgtcagc acgatcatgt
    49741 tgtcgccgcg atcgtcctga ttgataccga taatatcgaa caggttcatg gcgaaggcga
    49801 tcagattggt atcgtcatcc tgctcttcaa tgctttctgc cagtgcctgg gctttttcgc
    49861 caccgttgga atggatctcc agcaggcggt cacgcccctg ttccagttgt gctttcagcg
    49921 cttcgtgctg ctcgcggcag tttttgatca gatcgtcaaa gccttcggtc tcatccggtg
    49981 aagccagata gttaatcaga tcgttgtata cgctatcgta aatagtgcgt ccggtcgggc
    50041 aggtgtgctc gaacgcatcc agaccttcgt gataccagcg caccagcacc gactgagccg
    50101 ttttttccag ataaggcaca tggatctgaa tatcgtgcgc ctgaccgata cgatccagac
    50161 gcccaatacg ctgctccagc agatccgggt tgaatggcag gtcaaacatc accatgtggc
    50221 tggcgaactg gaagttacgt ccttcagaac cgatttccga gcacagcagt acctgcgcac
    50281 cggtgtcttc ttcagcaaac caggcggcag cgcggtcacg ttcgataatc gacatgcctt
    50341 cgtggaacac tgcggcgcga ataccttcac gttcgcgcag tacctgctcc agttgcagcg
    50401 cagtggccgc tttggcgcag atcaccagca ctttctgaga gcgatgactg gtcaggtagc
    50461 ccatcagcca ttcaacgcgc ggatcgaagt tccaccaggt ggcgttatcg ccttcaaatt
    50521 cctgataaat acgctccggg tagagcatat cgcgagcgcg atcttccgca cttttacgtg
    50581 cgcccataat gccggagact ttaatagcgg tctgatactg cgtcggcagt ggcagcttaa
    50641 tagtatgcag ctcgcgtttc gggaagcctt tcacaccgtt acgggtgtta cggaacagca
    50701 cgcggctggt accgtggcga tccatcagca tcgaaaccag ttcctgacgg gcgctctggg
    50761 catcttcgct gtcgctgttt gctgcttgca acagcggctc gatatcctgc tcgccgatca
    50821 tctcaccgag catgttcagt tcgtcattgc tgagtttgtt acccgccagc agcatggcaa
    50881 cggcgtccgc aaccggacga taatttttct gctcttcaac gaattgcgca aaatcgtgga
    50941 aacggttcgg gtccagcaga cgcagacggg cgaagtggct ttccatcccc agctgttccg
    51001 gggttgcggt cagcagcaga acgcccggca cgtgctctgc cagttgttca atggcctgat
    51061 attcacggct cggcgcatct tcgctccaca ccaggtgatg cgcttcatcg accaccagta
    51121 gatcccactc ggcttcacag agatgttcca gacgctgttt gctacgacgg gcaaaatcca
    51181 gcgagcaaat caccagctgt tcggtatcga acgggttgta agcatcgtgc tgagcttcgg
    51241 cataacgctc atcatcaaac agagcaaagc gcaggttgaa acggcgcagc atttctacca
    51301 gccactgatg ctgtaaggtt tccgggacga taatcagcac acgttcagca gcgccagaga
    51361 gcagttgctg atgcaggatc atcccggctt caatggtttt ccctaaaccc acttcgtcag
    51421 ccaacaggac gcgcggcgcg tggcggcgac caacatcatg agcgatgttg agctggtgcg
    51481 ggatcaggct ggtacgctga ccgcgcaggc cgctgtacgg catacggaac tgttcgctgg
    51541 aatacttacg cgcgcgataa cgcagcgcaa agcggtccat acggtcaatc tgcccggcaa
    51601 acagacggtc ctgcggtttg ctgaacacca gtttgctatc aaggaaaact tcacgcaggg
    51661 ccacgccgga ctcttcagta tcaaggcgag taccgatata ggtcagcaag ccattttctt
    51721 cttttacttc ttcgacttgc atctgccagc cgtcatggct ggtaatggta tcaccagggt
    51781 tgaacatcac gcgggtcacg ggggaatcac tgcgtgcgta cagacggttt tcaccagtag
    51841 atgggaaaag taaagtgaca gttcgcgcat ccaccgcgac aacggttcca agtcccaatt
    51901 cgctttctgt atcgctgatc cagcgttgac caagtgtaaa aggcatatgt gttcggctct
    51961 atatctttaa ttgcaggcaa taaccacccg ctaccgtgct tatgaggtag tggtgttatt
    52021 caggtccagg aatggaaagg gcgctatggt actggatggc aaagcattcg tcacgcatca
    52081 aaatggtatc tggcgaactc ttttttttgc tcaaaatagc ccaagttgcc cggtcataag
    52141 tgtagcaaaa ttatcctcaa taaaagggag tattccctcc gccacgggtt gtagctggcg
    52201 ggtcagatag tgttcgtaat ccagtggtga acgttggtag tccagcggct ccgggccgtt
    52261 ggtggtccat acgtacttaa tggtgccgcg gttctgatat tgcaaggggc gaccgcgctt
    52321 atggttttct tcatcggcaa ggcgagcggc gcgtacatga ggcggaacat tacgctgata
    52381 ttcgctcagc ggacggcgaa ggcgtttacg gtaaaccagc cgcgcatcca gttcgcccgc
    52441 catcagcttg tcgatggttt cgcgcacgta ttcctgatat ggctcgttgc ggaagatgcg
    52501 caggtaaagc tcctgctgaa actgctgggc cagcggcgtc cagtcggtgc gcacggtttc
    52561 cagcccttta aataccatcc gctgtttgtc gccctcctga atcagtccgg cgtagcgctt
    52621 tttgctgccg gtatcggccc cgcgaatggt tggcatcaga aaacggcaga aatgggtttc
    52681 atactccagt tccagtgcgc tggtcagccg ttgtttttgc agcgtatccg cccaccaggc
    52741 gttaacgtgc tgcaccagtg cacgaccgat tttcgctgct tcttcttccg aatgtgcacc
    52801 tttcagccag acaaacgttg agtcggtatc gccgtagata acatcatagc cctgcgcttc
    52861 aatcaacgtt ttggtttgcc gcatgatctg atgaccacgc atggtgatcg acgatgccag
    52921 gcgtggatcg aagaaacggc aggcggtggt gccgagcaca ccataaaagg cattcataat
    52981 gatcttcagc gcctgcgaaa gtggcttatt accctgacgt ttggcttcat cgcgcccgtg
    53041 ccagatgtta gtcacaatct ccggcaggca atgtttctcc cgtgagaacc aggcatcgag
    53101 aaaaccttcg gtactgtgct ctggatcagg ctgcgccatg ccttccacca gcccgacggg
    53161 atcaatcaga aaggtgcgga tgatcgacgg gtacaggctt ttgtagtcca gcaccagcac
    53221 cgaatcataa agcccaggtc gcgaatccat cacgtagccg ccagggctgg cgtgcggcgg
    53281 cacttcgccg agattaggcg cgacataacc agcgcgatgc attcggggaa aatagagatg
    53341 accaaacgct gccaccgaac cgccgtgtcg atccaccggc aggccgttca ccgtcgcccg
    53401 ttcgagcaaa aacggcatga tttcagtttt gtggaagatc tgcgtcacca gctcgcaatc
    53461 tttcaggtta taagttgcca gcgcaggctt atcttcggcg aaacggcggt caatttcgtc
    53521 cattcgatcc cacgggttat cgatagattt tccttcgcct aataactcct gagcgacggt
    53581 ttccagcgag aatgaagaga aattccagaa cgcggatttc agcgcctcga taccgtcgat
    53641 aattagccga cctttaacct gagcaaaaaa cacaccgttt ttaaagccgt gctcgcgcca
    53701 ctccagctca ctgttatcac gccccagcct cagcggaata cggtaacgct cggcatgttt
    53761 ttgcagcatt cgcagatcaa actgcaccac gttccagccg atgattacat cgggatcatg
    53821 agtcgcaaac cagacgttaa gtttttccag tagctgcggg cggctggcga cgtattccag
    53881 ttcaaaatcg agcgcggaga cgtcgccatt ctctggcccc agcatataaa cgatacgctg
    53941 cccgcaacct tccaggccga tgcagtacag ctcaccgtgg cgggtggttt caatatcaat
    54001 agaaacccac ttgagcggcg gacgatagtc gggatgcggt ttcagacggg cattaacgat
    54061 agcgccattg cgcatatcac cctcgaccca caccggtgag gtgataaacc gctccatcag
    54121 atagcgttct ggcgggcgca catcggcctc gtagacggta acgccacctt cacgcaggcg
    54181 cttttcgtaa ttcatcaact ggcgatgggc gcgacagtaa aggccataca ccggctggcg
    54241 gtgaaaatct tttaacgcca gcggcgtcag gcgaaagcct tgttcaccct gcaaaatatg
    54301 ctgagcgcgg ggaacctgat cggcaggaat aaacgccacg gactcttgcg gcgcaaggaa
    54361 aacctgcaac ggcccgttgt ccgtcgccag ccagaaggaa acttcggtcc cttgcggggt
    54421 gtcccgccag tgtcgggtta agataaaacc tgcctgcgcc acgctgaaaa tccaataaaa
    54481 aaccaggctt gattatagcc tggtttcgtt tgattggctg tggttttata cagtcattac
    54541 tgcccgtaat atgccttcgc gccatgctta cgcagatagt gtttatccag cagcgtttgc
    54601 tgcatatccg gtaactgcgg cgctaactga cggcagaata tacccatata agcgacctct
    54661 tccagcacga tggcattatg caccgcatct tcggcatttt tgccccatgc aaatgggccg
    54721 tgagaatgga ccaggacgcc gggcatttgc gctgcatcga taccctgttt ttcgaaggtt
    54781 tctacgatga cgttaccggt ttcccactca tattcaccgt tgatttctgc gtcggtcatt
    54841 ttgcgggtgc agggaatggg accgtagaaa tagtcggcgt gggtggtacc ggttgccgga
    54901 attgactggc ccgcctgcgc ccagatggtg gcgtggcgcg agtgtgtgtg cacaatgccg
    54961 ccaatagacg ggaatgcctg atagagcagc cggtgagttg gcgtgtcgga ggagggcttt
    55021 ttcgtacctt caaccacttc accggtttcg atgctgacca cgaccatatc gtcagcggtc
    55081 atgatgctgt agtcgacgcc ggaaggtttg atcacaaaga cgccgcgccc gcgatcaacg
    55141 gcgctgacat tgccccaggt gagcgtgacc aggttgtgtt ttggcagcgc caggttggct
    55201 tctaatacct ggcgtttgag atcttctaac atgttgactc cttcgtgccg gatgcgcttt
    55261 gcttatccgg cctacaaaat cgcggcgtgt aggcctgata agacgcgtcg gcgtcgcatc
    55321 aggcggtgaa tgccggatgc gctttgctta tccggcctac aaatccggaa cgcgtaggcc
    55381 tgataagacg cgccagcgtc gcatcaggcg ttacatacct gatgcggcta cttagcgacg
    55441 aaatccgtaa tacacttcgt tccagcgcag cgcgtcttta aacgctggaa ggcgggtatc
    55501 gttatcaatc accgtgattt caatgtcgtg catctcggcg aactggcgca tatcgttgag
    55561 gttcagcgca tggctgaaga cggtatggtg cgcgccaccg gcaaggatcc acgcttcgga
    55621 agcggtcggc agatccggtt gcgctttcca cagcgcattc gccaccggca gtttcggcag
    55681 ggagtgcggt gttttcaccg tgtcgatgca gttaaccagc agacgataac gatcgccgag
    55741 atcaatcagg ctggcgacaa tggccggacc agtttgagta ttgaagatca ggcgagctgg
    55801 gtcgtcttta ccgccaatac caaggtgctg aacgtcgagg atcggtttct cttccacagc
    55861 gatcgacggg cagacttcca gcatatggga gccgagtacc aggtcattac ctttctcgaa
    55921 gtgataggtg tagtcctcca taaaggaggt gccgccctgc agaccggttg acatcacctt
    55981 catgatgcga agcagggcgg cagttttcca gtcgccttcg cccgcaaagc cgtagccctg
    56041 ctgcatcaga cgctgtacgg ccagacccgg aagctgtttc agaccgtgca aatcttcaaa
    56101 ggtggtggtg aacgcgtgga agccaccttg ttccaggaaa cgcttcatcc ccagctcaat
    56161 acgcgccgct tccagcacgt tctgtcgttt ttcgccgtgg atttgtgtgg caggtgtcat
    56221 ggtgtagcag ctttcgtact catcgaccag cgcgttaaca tcgccgtcgc tgatggagtt
    56281 caccacctgc accagatcgc caaccgccca ggtattgacg gagaaaccaa acttgatctg
    56341 tgcggcaact ttatcgccat cggtgaccgc tacttcacgc atgttatcgc caaaacggca
    56401 gactttcaga tgacgggtat cctgtttaga gaccgcctga cgcatccagg agccgatacg
    56461 ctcatgtgct tgtttatcct gccagtgacc ggtaacaacg gcatgttgct gacgcatacg
    56521 cgtgccgatg aagccgaact cgcgaccgcc atgcgcggtc tggttcaggt tcataaagtc
    56581 catgtcgatg ctgtcccacg gcagcgccgc gttgaactgg gtgtggaatt gcagcaacgg
    56641 tttgttgagc atggtcaggc cgttgatcca cattttggcc ggggagaaag tgtgcagcca
    56701 caccaccaga ccagcgcaac gatcgtcgta attcgcgtcg cggcaaatag cggtgatttc
    56761 atccggcgtg gtgcccagcg gtttcagcac cagtttgcag ggcaacttcg cttctgtatt
    56821 cagtgcatta acaacgtgtt ccgcatgttg cgtcacctgg cgcagagtct ccgggccgta
    56881 aagatgctgg ctgccaatta caaaccacac ttcataatta tcaaaaatcg tcattgtcgt
    56941 gtccttatag agtcggaacg gcctgggcag cctgtgccgg ggcggaagtt ggaagatagt
    57001 gttgttcgac gcttatcgcc cattgctgat agcggcgata aagctgttca aagcgttgtg
    57061 cctgttcgct gcgcggttgc agggttttct ctaccgcact ggccattttt tgctgggctg
    57121 atgggatgtc tgcgtgcact ttcgcggcga cagcagcaaa aatcgccgca ccgagcgcac
    57181 agcactggtc agaggcaaca atttgcagcg ggcgattcag cacgtcgcag caggcctgca
    57241 taatgacctg gtttttccgc gcgatgccgc ccagcgccat cacgttattg acggcgatcc
    57301 cctgatcggt aaagcactcc atgattgcgc gtgcgccaaa ggcggtggca gcaatcaaac
    57361 cgccgaacag cagcggagcg tcggtagcga ggttcagatc ggtaatcacc cctttcaggc
    57421 gttggttagc gttcggtgtg cggcggccgt taaaccagtc gagcaccacc ggtaggtgat
    57481 ccagagacgg attttgagcc catgcttcgg tcagcgccgg aagcagttgt ttctggctgg
    57541 cgttgatttg ctcttttagt tccggatgct gggcggcaag ctgttccagc ggccagccga
    57601 gtacgcgacc gaaccaggcg tagatatccc caaacgccga ttggcctgct tccagaccga
    57661 taaacccagg caccacgctg ccatcaacct gaccgcaaat gcctttcact gcccgctcgc
    57721 caacgctctg tttgtcggca atcagaatgt cgcaggtgga agtaccgata acttttacca
    57781 gtgcgttagg ctgtgcgcct gcgccaactg cgcccatatg gcagtcaaac gcgccaccgg
    57841 aaatcaccac gctttcaggc aggccgagac gctgcgccca ttccgggcat aaggtgccaa
    57901 ccggaatatc ggcagtccag gtatcagtga acagcgggga aggcaaatgg cgattgagga
    57961 tcgggtccag ctcatcaaag aaactggctg gcggcaggcc accccagctt tcgtgccaca
    58021 gagatttatg cccggcgctg caacgtccgc gacgaatatc ctgcgggcgg gtggtaccgg
    58081 aaagcagagc tggcacccag tcgcacagct caatccatga tgcggcagat tgtgccacgg
    58141 cgttgtcctg gcgagttaca tgcaggattt ttgcccagaa ccattcgctg gaataaatac
    58201 cgccaatata gcgggagtag tcaacgttgc ccggcgtgtg acataagcgg gtaatctctt
    58261 ccgcttcttc aaccgcggtg tggtctttcc acaatacgaa catcgcgttc gggttctcgg
    58321 caaactccgg gcgcagcgcc aacacgttgc cgtcggcgtc aatcggtgcg ggcgtcgagc
    58381 cggttgtgtc aacgccaatc ccgaccacag ctgcgcgctg ttcgacgcta agctctgcaa
    58441 gcacggtttt cagcgccgct tccattgact caatgtagtc acgcggatga tgacggaact
    58501 ggttattcgg ggcatcacaa aattgcccgt tctgccaacg gggataccac tctacgctgg
    58561 tggcgatctc ttcaccggtg gcacattcca ccgccaaagc tcgcacagaa tcactgccaa
    58621 aatcgaggcc aattgcaatc gccatcgttt cactccatcc aaaaaaacgg gtatggagaa
    58681 acagtagaga gttgcgaaaa aaaacgtcag gctggatccg ctaatcttat ggataaaaat
    58741 gctatggcat agcaaagtgt gacgccgtgc aaataatcaa tgtggacttt tctgccgtga
    58801 ttatagacac ttttattacg cgtttttgtc atggctttgg tcccgctttg ttacagaatg
    58861 cttttaataa gcggggttac cggttgggtc agcgagaaga gccagtaaaa gacgcagtga
    58921 cggcaatgtc tgatgcaata tggacaattg gtttcttctc tgaatggcgg gagtatgaaa
    58981 agtatggctg aagcgcaaaa tgatcccctg ctgccgggat actcgtttaa cgcccatctg
    59041 gtggcgggtt taacgccgat tgaggccaac ggttatctcg atttttttat cgaccgaccg
    59101 ctgggaatga aaggttatat tctcaatctc accattcgcg gtcagggcat agtgaaaaac
    59161 cagggacgag aatttgcctg ccgaccgggt gatattttgc tgttcccgcc aggagagatt
    59221 catcactacg gtcgtcatcc ggaggctcac gaatggtatc accagtgggt ttactttcgt
    59281 ccgcgcgcct actggcatga atggcttaac tggccgtcga tatttgccaa taccggtttc
    59341 tttcgcccgg atgaagcgca ccagccgcat ttcagcgacc tgtttgggca aatcattaat
    59401 gccggacaag gggaagggcg ctattcggaa ttgctggcga taaatctgct tgagcaattg
    59461 ttactgcggc gcatggaagc gattaacgag tcgcttcatc cgccgatgga taatcgggta
    59521 cgcgaggctt gtcagtacat cagcgatcac ctggcagaca gtaattttga tatcgccagc
    59581 gtcgcacagc atgtctgcct gtcgccgtcg cgtctgtcgc atttgttccg ccagcagtta
    59641 gggattagcg tattaagctg gcgcgaggac cagcgcatca gtcaggcgaa gctgcttttg
    59701 agcaccaccc ggatgcctat cgccacagtc ggtcgcaatg tcggttttga cgatcaactc
    59761 tatttctcgc gagtatttaa aaaatgcacc ggggccagcc cgagcgagtt tagggctgga
    59821 tgtgaataat aagaaagaag ggggagtctc ttactttcg
//
LOCUS       DAHHTG010000009        58431 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.009, whole genome shotgun sequence.
ACCESSION   DAHHTG010000009 DAHHTG010000000
VERSION     DAHHTG010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 58431)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 58431)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..58431
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.009"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            complement(<1..153)
                     /locus_tag="NBI74_000592"
     CDS             complement(<1..153)
                     /locus_tag="NBI74_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000658729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1427 family protein"
                     /protein_id="HCD1930968.1"
                     /translation="MKDILFSSGVGFGIGALFTIVRLPIPVPNVLPGILSIVFMYVGY
                     LVVKYFM"
     gene            complement(206..1462)
                     /gene="xapB"
                     /locus_tag="NBI74_000593"
     CDS             complement(206..1462)
                     /gene="xapB"
                     /locus_tag="NBI74_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthosine/proton symporter XapB"
                     /protein_id="HCD1930969.1"
                     /translation="MSIALRLKVMSFLQYFIWGSWLVTLGSYMINTLHFTGANVGMVY
                     SSKGIAAIIMPGIMGIIADKWLRAERAYMLCHLVCAGVLFYAASVTDPDMMFWVMLVN
                     AMAFMPTIALSNSVSYSCLAQAGLDPVTAFPPIRVFGTVGFIVAMWAVSLLHLELSSL
                     QLYIASGASLLLSAYALTLPKIPVAEKKATTSLASKLGLDAFVLFKNPRMAIFFLFAM
                     MLGAVLQITNVFGNPFLHDFARNPEFADSFVVKYPSILLSVSQMAEVGFILTIPFFLK
                     RFGIKTVMLMSMVAWTLRFGFFAYGDPSPTGFILLLLSMIVYGCAFDFFNISGSVFVE
                     QEVDSSIRASAQGLFMTMVNGVGAWVGSILSGMAVDYFSVDGVKDWHTIWLMFAGYAL
                     FLAVIFFFGFKYNHDPEKITHRSVTH"
     gene            complement(1523..2356)
                     /gene="xapA"
                     /locus_tag="NBI74_000594"
     CDS             complement(1523..2356)
                     /gene="xapA"
                     /locus_tag="NBI74_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthosine phosphorylase"
                     /protein_id="HCD1930970.1"
                     /translation="MYQAQFSHNPLYCVDIIKTYKPDFTPRVAFILGSGLGALADQIE
                     NAVAISYEKLPGFPVSTVHGHAGELVLGYLQGVPVVCMKGRGHFYEGRGMTIMTDAIR
                     TFKLLGCELLFCTNAAGSLRPEVGAGSLVALKDHINTMPGTPMVGLNDERFGERFFSL
                     ANAYDAEYRALLQKVAKEEGVPLTEGVFVSYPGPNFETAAEIRMMQIIGGDVVGMSVV
                     PEVISARHCELKVVAVSAITNMAEGLSDVKLSHAQTLAAAELSKQNFINLICGFLRKI
                     A"
     gene            2605..3369
                     /locus_tag="NBI74_000595"
     CDS             2605..3369
                     /locus_tag="NBI74_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1930971.1"
                     /translation="MKKHLFTLTLSSVLAIPAVSHAEFKGGFADIGIHYLDWTSRTTE
                     KSSTKSHKDDFGYLELEGGANFSWGEMYGFFDWENFYNDRHDKPGSEQRYTFKNTNRI
                     YLGDTGFNLYLHAYGTYGSANRVNFHDDMFLYGIGYNFTGSGWWFKPFFAKRYTDQTY
                     YTGDNGYVAGWVAGYSFMLGSEKFTLTNWNEYEFDRDATYAAGNGGKEGLNGAVALWW
                     NATSHITTGIQYRYADDKLGEDFYQDAIIYSIKFNF"
     gene            complement(3408..4334)
                     /locus_tag="NBI74_000596"
     CDS             complement(3408..4334)
                     /locus_tag="NBI74_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCD1930972.1"
                     /translation="MNYSLKQLKVFVTVAQEKSFSRAGERIGLSQSAVSHSVKELENH
                     TGVRLLDRTTREVVLTDAGQQLALRLERLLDELNSTLRDTGRMGQQLSGKVRVAASQT
                     ISAHLIPQCIAESHRRYPDIQFVLHDRPQQWVMESIRQGDVDFGIVIDPGPVGDLQCE
                     AILSEPFFLLCHRDSALAVEDYVPWQALQGAKLVLQDYASGSRPLIDAALARNGIQAN
                     IVQEIGHPATLFPMVAAGIGISILPALALPLPEGSPLVVKRITPVVERQLMLVRRKNR
                     SLSTAAEALWDVVRDQGNALMAGREGDPLYQI"
     gene            4424..5422
                     /locus_tag="NBI74_000597"
     CDS             4424..5422
                     /locus_tag="NBI74_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter"
                     /protein_id="HCD1930973.1"
                     /translation="MKLFRILDPFTLTLITVVLLASFFPARGDFVPFFENLTTAAIAL
                     LFFMHGAKLSREAIIAGGGHWRLHLWVMCSTFVLFPILGVLFAWWKPVNVDPMLYSGF
                     LYLCILPATVQSAIAFTSMAGGNVAAAVCSASASSLLGIFLSPLLVGLVMNVHGAGGS
                     LEQVGKIMLQLLLPFVLGHLSRPWIGDWVSRNKKWIAKTDQTSILLVVYTAFSEAVVN
                     GIWHKVGWGSLLFIVVVSCVLLAIVIVVNVFMARRLGFNKADEITIVFCGSKKSLANG
                     IPMANILFPTSVIGMMVLPLMIFHQIQLMVCAVLARRYKRQTEQLQAQQESSADKA"
     gene            complement(5419..5637)
                     /locus_tag="NBI74_000598"
     CDS             complement(5419..5637)
                     /locus_tag="NBI74_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3820 family protein"
                     /protein_id="HCD1930974.1"
                     /translation="MEKEQLIEIANTIMPFGKYKGRRLIDLPEEYLLWFARKDEFPAG
                     KLGELMQITLLIKIEGLTQLVQPLKRPL"
     gene            complement(5639..7654)
                     /gene="ligA"
                     /locus_tag="NBI74_000599"
     CDS             complement(5639..7654)
                     /gene="ligA"
                     /locus_tag="NBI74_000599"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HCD1930975.1"
                     /translation="MESIEQQLTELRTTLRHHEYLYHVMDAPEIPDAEYDRLMRELRE
                     LETKHPELITPDSPTQRVGAAPLAAFSQIRHEVPMLSLDNVFDEESFLAFNKRVQDRL
                     KSNEKVTWCCELKLDGLAVSILYENGVLVSAATRGDGTTGEDITSNVRTIRAIPLKLH
                     GENIPARLEVRGEVFLPQAGFEKINEDARRTGGKVFANPRNAAAGSLRQLDPRITAKR
                     PLTFFCYGVGVLEGGELPDTHLGRLMQFKAWGLPVSDRVTLCESAEEVLAFYHKVEKD
                     RPTLGFDIDGVVIKVNSLAQQEQLGFVARAPRWAVAFKFPAQEQMTFVRDVEFQVGRT
                     GAITPVARLEPVHVAGVLVSNATLHNADEIERLGLRIGDKVVIRRAGDVIPQVVNVVL
                     SERPEDTREVVFPTHCPVCGSDVERVEGEAVARCTGGLICGAQRKESLKHFVSRRAMD
                     VDGMGDKIIDQLVEKEYVHTPADLFKLTAGKLTGLERMGPKSAQNVVNALEKAKETTF
                     ARFLYALGIREVGEATAAGLAAYFGTLEALEAASIEELQKVPDVGIVVASHVHNFFAE
                     ESNRNVISELLAEGVHWPEPIVINAEEIDSPFAGKTVVLTGSLSQMSRDDAKARLVEL
                     GAKVAGSVSKKTDLVIAGEAAGSKLAKAQELGIEVIDETEMLRLLGS"
     gene            complement(7725..8723)
                     /gene="zipA"
                     /locus_tag="NBI74_000600"
     CDS             complement(7725..8723)
                     /gene="zipA"
                     /locus_tag="NBI74_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZipA"
                     /protein_id="HCD1930976.1"
                     /translation="MMQDLRLILIIVGAIAIIALLVHGFWTSRKERSSMFRDRPLKRM
                     KSKRDDDSYDEDVEDDEGVGEVRVHRVNHAPANAQEHEVARPSPQHQYQPPYASAQPR
                     QPVQQPPEAQVPPQHAPRPTQPVQQPVQQPAYQPQPEQPLQQPVSPQVASAPQPVHSA
                     PQPAQQAFQPAEPVAAPQPEPVAEPAPVMDKPKRKEAVIIMNVAAHHGSELNGELLLN
                     SIQQAGFIFGDMNIYHRHLSPDGSGPALFSLANMVKPGTFDPEMKDFTTPGVTIFMQV
                     PSYGDELQNFKLMLQSAQHIADEVGGVVLDDQRRMMTPQKLREYQDIIREVKDANA"
     gene            8953..9714
                     /gene="cysZ"
                     /locus_tag="NBI74_000601"
     CDS             8953..9714
                     /gene="cysZ"
                     /locus_tag="NBI74_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate transporter CysZ"
                     /protein_id="HCD1930977.1"
                     /translation="MVSSFTSAPRSGFYYFAQGWKLVSQPGIRRFVILPLLVNILLMG
                     GAFWWLFTQLDVWIPTLMSYVPDWLQWLSYLLWPLAVISVLLVFGYFFSTIANWIAAP
                     FNGLLAEQLEARLTGATPPDTGIFGIMKDVPRIMKREWQKFAWYLPRAIVLLILYFIP
                     GIGQTVAPVLWFLFSAWMLAIQYCDYPFDNHKVPFKEMRTALRTRKITNMQFGALTSL
                     FTMIPLLNLFIMPVAVCGATAMWVDCYRDKHAMWR"
     gene            9899..10870
                     /gene="cysK"
                     /locus_tag="NBI74_000602"
     CDS             9899..10870
                     /gene="cysK"
                     /locus_tag="NBI74_000602"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HCD1930978.1"
                     /translation="MSKIFEDNSLTIGHTPLVRLNRIGNGRILAKVESRNPSFSVKCR
                     IGANMIWDAEKRGVLKPGVELVEPTSGNTGIALAYVAAARGYKLTLTMPETMSIERRK
                     LLKALGANLVLTEGAKGMKGAIQKAEEIVASNPEKYLLLQQFSNPANPEIHEKTTGPE
                     IWEDTDGQVDVFIAGVGTGGTLTGVSRYIKGTKGKTDLISVAVEPTDSPVIAQALAGE
                     EIKPGPHKIQGIGAGFIPANLDLKLVDKVIGITNEEAISTARRLMEEEGILAGISSGA
                     AVAAALKLQEDESFTNKNIVVILPSSGERYLSTALFADLFTEKELQQ"
     gene            11254..11511
                     /gene="ptsH"
                     /locus_tag="NBI74_000603"
     CDS             11254..11511
                     /gene="ptsH"
                     /locus_tag="NBI74_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003038086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein Hpr"
                     /protein_id="HCD1930979.1"
                     /translation="MFQQEVTITAPNGLHTRPAAQFVKEAKGFTSEITVTSNGKSASA
                     KSLFKLQTLGLTQGTVVTISAEGEDEQKAVEHLVKLMAELE"
     gene            11556..13283
                     /gene="ptsI"
                     /locus_tag="NBI74_000604"
     CDS             11556..13283
                     /gene="ptsI"
                     /locus_tag="NBI74_000604"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate-protein phosphotransferase
                     PtsI"
                     /protein_id="HCD1930980.1"
                     /translation="MISGILASPGIAFGKALLLKEDEIIIDRKKISADQVDQEVERFL
                     SGRAKASAQLETIKTKAGETFGEEKEAIFEGHIMLLEDEELEQEIIALIKDKHMTADA
                     AAHEVIEGQASALEELDDEYLKERAADVRDIGKRLLRNILGLKIIDLSAIQDEVILVA
                     ADLTPSETAQLNLKKVLGFITDAGGRTSHTSIMARSLELPAIVGTGSVTSQVKNDDYL
                     ILDAVNNQVYVNPTNEVIDKMRAVQEQVASEKAELAKLKDLPAITLDGHQVEVCANIG
                     TVRDVEGAERNGAEGVGLYRTEFLFMDRDALPTEEEQFAAYKAVAEACGSQAVIVRTM
                     DIGGDKELPYMNFPKEENPFLGWRAIRIAMDRKEILRDQLRAILRASAFGKLRIMFPM
                     IISVEEVRALRKEIEIYKQELRDEGKAFDESIEIGVMVETPAAATIARHLAKEVDFFS
                     IGTNDLTQYTLAVDRGNDMISHLYQPMSPSVLNLIKQVIDASHAEGKWTGMCGELAGD
                     ERATLLLLGMGLDEFSMSAISIPRIKKIIRNTNFEDAKVLAEQALAQPTTDELMTLVN
                     KFIEEKTIC"
     gene            13324..13833
                     /gene="crr"
                     /locus_tag="NBI74_000605"
     CDS             13324..13833
                     /gene="crr"
                     /locus_tag="NBI74_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015833547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HCD1930981.1"
                     /translation="MGLFDKLKSLVSDDKKDTGTIEIIAPLSGEIVNIEDVPDVVFAE
                     KIVGDGIAIKPTGNKMVAPVDGTIGKIFETNHAFSIESDSGVELFVHFGIDTVELKGE
                     GFKRIAEEGQRVKVGDTVIEFDLPLLEEKAKSTLTPVVISNMDEIKELIKLSGSVTVG
                     ETPVIRIKK"
     gene            complement(13876..14727)
                     /gene="pdxK"
                     /locus_tag="NBI74_000606"
                     /gene_synonym="thiJ"
     CDS             complement(13876..14727)
                     /gene="pdxK"
                     /locus_tag="NBI74_000606"
                     /gene_synonym="thiJ"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine/pyridoxal/pyridoxamine kinase"
                     /protein_id="HCD1930982.1"
                     /translation="MSSLLLFNDKSRALQADIVAVQSQVVYGSVGNSIAVPAIKQNGL
                     NVFAVPTVLLSNTPHYDTFYGGAIPDEWFSGYLRALQERDALRQLRAVTTGYMGTASQ
                     IKILAEWLTALRKDHPDLLIMVDPVIGDIDSGIYVKPDLPEAYRQYLLPLAQGITPNI
                     FELEILTGKDCRDLDSAIAAAKSLLSDTLKWVVITSASGNEENQEMQVVVVSADSVNV
                     ISHSRVKTDLKGTGDLFCAQLISGLLKGKALTDAVHRAGLRVLEVMRYTQQHESDELI
                     LPPLAEA"
     gene            14832..15200
                     /locus_tag="NBI74_000607"
     CDS             14832..15200
                     /locus_tag="NBI74_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeK family protein"
                     /protein_id="HCD1930983.1"
                     /translation="MKKIICLVVTLLMTLPAYAKLTAHEEARINAMLEGLAQKKDLIF
                     VRNGDEHTCDEAVSHLRLKLGNTRNRIDTAEQFIDKVASSSSITGKPYIVKMPGKSDE
                     NAQPFLHALIAQTDKTVPAQ"
     gene            complement(15203..16114)
                     /gene="cysM"
                     /locus_tag="NBI74_000608"
     CDS             complement(15203..16114)
                     /gene="cysM"
                     /locus_tag="NBI74_000608"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase B"
                     /protein_id="HCD1930984.1"
                     /translation="MSTLEQTIGNTPLVKLQRMAPDNGSEVWLKLEGNNPAGSVKDRA
                     ALSMIVEAEKRGEIKPGDVLIEATSGNTGIALAMIAALKGYRMKLLMPDNMSQERRAA
                     MRAYGAELILVTKEQGMEGARDLALEMANRGEGKLLDQFNNPDNPYAHYTTTGPEIWQ
                     QTGGRITHFVSSMGTTGTITGVSRFMREQSKPVTIVGLQPEEGSSIPGIRRWPAEYLP
                     GIFNASLVDEVLDIHQRDAENTMRELAVREGIFCGVSSGGAVAGALRVAKANPGAVVV
                     AIICDRGDRYLSTGVFGEEHFSQGAGI"
     gene            complement(16249..17346)
                     /gene="cysA"
                     /locus_tag="NBI74_000609"
     CDS             complement(16249..17346)
                     /gene="cysA"
                     /locus_tag="NBI74_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter ATP-binding
                     protein CysA"
                     /protein_id="HCD1930985.1"
                     /translation="MSIEIANIKKSFGRTQVLNDISLDIPSGQMVALLGPSGSGKTTL
                     LRIIAGLEHQTSGHIRFHGTDVSRLHARDRKVGFVFQHYALFRHMTVFDNIAFGLTVL
                     PRRERPNAAAIKAKVTKLLEMVQLAHLADRYPAQLSGGQKQRVALARALAVEPQILLL
                     DEPFGALDAQVRKELRRWLRQLHEELKFTSVFVTHDQEEATEVADRVVVMSQGNIEQA
                     DAPDQVWREPATRFVLEFMGEVNRLQGTIRGGQFHVGAHRWPLGYTPAYQGPVDLFLR
                     PWEVDISRRTSLDSPLPVQVLEASPKGHYTQLVVQPLGWYNEPLTVVMHGDDAPQRGD
                     RLYVGLQHARLYNGDERIETRDEELALAQSA"
     gene            complement(17336..18211)
                     /gene="cysW"
                     /locus_tag="NBI74_000610"
     CDS             complement(17336..18211)
                     /gene="cysW"
                     /locus_tag="NBI74_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysW"
                     /protein_id="HCD1930986.1"
                     /translation="MAEVTQLKRYDARPINWGKWFLIGIGMLVSAFILLVPMIYIFVQ
                     AFSKGLMPVLQNLADPDMLHAIWLTVMIALIAVPVNLVFGILLAWLVTRFNFPGRQLL
                     LTLLDIPFAVSPVVAGLVYLLFYGSNGPLGGWLDEHNLQIMFSWPGMVLVTIFVTCPF
                     VVRELVPVMLSQGSQEDEAAILLGASGWQMFRRVTLPNIRWALLYGVVLTNARAIGEF
                     GAVSVVSGSIRGETLSLPLQIELLEQDYNTVGSFTAAALLTLMAIITLFLKSMLQWRL
                     ENQEKRAQQEEHHEH"
     gene            complement(18211..19044)
                     /gene="cysT"
                     /locus_tag="NBI74_000611"
     CDS             complement(18211..19044)
                     /gene="cysT"
                     /locus_tag="NBI74_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysT"
                     /protein_id="HCD1930987.1"
                     /translation="MFAVSSRRVLPGFTLSLGTSLLFVCLILLLPLSALVMQLAQMSW
                     AQYWEVITNPQVVAAYKVTLLSAFVASIFNGVFGLLMAWILTRYRFPGRTLLDALMDL
                     PFALPTAVAGLTLASLFSVNGFYGEWLAKFDIKVTYTWLGIAVAMAFTSIPFVVRTVQ
                     PVLEELGPEYEEAAETLGATRWQSFRKVVLPELSPALVAGVALSFTRSLGEFGAVIFI
                     AGNIAWKTEVTSLMIFVRLQEFDYPAASAIASVILAASLLLLFSINTLQSRFGRRVVG
                     H"
     gene            complement(19044..20060)
                     /gene="cysP"
                     /locus_tag="NBI74_000612"
     CDS             complement(19044..20060)
                     /gene="cysP"
                     /locus_tag="NBI74_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate/sulfate ABC transporter
                     substrate-binding protein CysP"
                     /protein_id="HCD1930988.1"
                     /translation="MAVNLLKKNSLVLVASLLLAGHVQATELLNSSYDVSRELFAALN
                     PPFEQQWAKDNGGDKLTIKQSHAGSSKQALAILQGLKADVVTYNQVTDVQILHDKGKL
                     IPADWQSRLPNNSSPFYSTMGFLVRKGNPKNIHDWNDLVRSDVKLIFPNPKTSGNARY
                     TYLAAWGAADKADGGDKAKTEQFMTQFLKNVEVFDTGGRGATTTFAERGLGDVLISFE
                     SEVNNIRKQYEAQGFEVVIPKTNILAEFPVAWVDKNVQANGTEKAAKAYLNWLYSPQA
                     QTIITDYYYRVNNPEVMGKLKDKFPQTELFRVEDKFGSWPEVMKTHFTSGGELDKLLA
                     AGRK"
     gene            complement(20218..21009)
                     /gene="ucpA"
                     /locus_tag="NBI74_000613"
     CDS             complement(20218..21009)
                     /gene="ucpA"
                     /locus_tag="NBI74_000613"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase UcpA"
                     /protein_id="HCD1930989.1"
                     /translation="MGKLTGKTALITGALQGIGEGIARTFARHGANLILLDISPEIEK
                     LADELCGRGHRCTAVVADVRDPASVAAAIKRAKEKEGRIDILVNNAGVCRLGSFLDMS
                     DEDRDFHIDINIKGVWNVTKAVLPEMIARKDGRIVMMSSVTGDMVADPGETAYALTKA
                     AIVGLTKSLAVEYAQSGIRVNAICPGYVRTPMAESIARQSNPEDPESVLTEMAKAIPM
                     RRLADPLEVGELAAFLASDESSYLTGTQNVIDGGSTLPETVSVGI"
     gene            21289..22185
                     /gene="murQ"
                     /locus_tag="NBI74_000614"
     CDS             21289..22185
                     /gene="murQ"
                     /locus_tag="NBI74_000614"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HCD1930990.1"
                     /translation="MQLEKMITEGSNAASAEIDRVSTLEMCRIINDEDKTVPLAVERV
                     LPDIAAAIDVIHTQVSGGGRLIYLGAGTSGRLGILDASECPPTYGVKPGLVVGLIAGG
                     EYAIQHAVEGAEDSREGGVNDLKNIGLTAQDVVVGIAASGRTPYVIAGLEYARQLGCR
                     TVGISCNPGSAVSSTAEFAITPVVGAEVVTGSSRMKAGTAQKLVLNMLSTGLMIKSGK
                     VFGNLMVDVVATNEKLHVRQVNIVKNATGCNAEQAEAALIACERNCKTAIVMVLKNLD
                     AAEAKKCLDQHGGFIRKALEKE"
     gene            22189..23613
                     /gene="murP"
                     /locus_tag="NBI74_000615"
     CDS             22189..23613
                     /gene="murP"
                     /locus_tag="NBI74_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylmuramic acid transporter subunit
                     IIBC"
                     /protein_id="HCD1930991.1"
                     /translation="MAKEISSELLNTILTRVGGPGNIASCGNCMTRLRLGVHDSSLVD
                     PNIKTLEGVKGVILTSDQVQVVFGPGKAHRAAKAMSELLGEAPVQDAAEIAAQNKRQL
                     KARQTSGVQQFLAKFATIFTPLIPGFIAAGLLLGIATLIATVMHVPADAQGTLPDALN
                     FMKVFSKGLFTFLVILVGYNAAQAFGGTGVNGAIIAALFLLGYNPAATTGYYAGFHDF
                     FGLPIDPRGNIIGVLIAAWACARIECMVRRFMPDDLDMLLTSLITLLITATLAYLIIM
                     PLGGWLFEGMSWLFMHLNSNPLGCAVLAGLFLIAVVFGVHQGFIPVYLALMDSQGFNS
                     LFPILSMAGAGQVGAALALYWRAQPHSALRSQVRGAIIPGLLGVGEPLIYGVTLPRMK
                     PFVTACLGGAAGGLFIGLIAWWGLPMGLNSAFGPSGLVALPLMTSAQGILPAMAVYAG
                     GILVAWVSGFIFTTLFGCRNVNLD"
     gene            complement(23791..24690)
                     /gene="yfeX"
                     /locus_tag="NBI74_000616"
     CDS             complement(23791..24690)
                     /gene="yfeX"
                     /locus_tag="NBI74_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311329.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphyrinogen peroxidase"
                     /protein_id="HCD1930992.1"
                     /translation="MSQVQSGILPEHCRAAIWIEANVKGEVDALRAASKTFADKLATF
                     EAKFPDAHLGAVVAFGNNTWRALSGGVGAEELKDFPGYGKGLAPTTQFDVLIHILSLR
                     HDVNFSVAQAAMEAFGDCIEVKEEIHGFRWVEERDLSGFVDGTENPAGEETRREVAVI
                     KDGVDAGGSYVFVQRWEHNLKQLNRMSVHDQEMMIGRTKEANEEIDGDERPETSHLTR
                     VDLKEDGKGLKIVRQSLPYGTASGTHGLYFCAYCARLHNIEQQLLSMFGDTDGKRDAM
                     LRFTKPVTGGYYFAPSLDKLMAL"
     gene            complement(24786..25361)
                     /locus_tag="NBI74_000617"
     CDS             complement(24786..25361)
                     /locus_tag="NBI74_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpoE-regulated lipoprotein"
                     /protein_id="HCD1930993.1"
                     /translation="MKSLRLMLCAMPLMLTGCSTMSSVNWSAANPWNWFGSSTKVSEQ
                     GVGELTASTPLQEQAIADALDGDYRLRSGMKTANGNVVRFFEVMKGNNVAMVINGDQG
                     TISRIDVLDSDIPADTGVKIGTPFSDLYSKAFGNCQKADSDDNRAVECKAEGSQHISY
                     QFSGEWSGPEGLMPSDDTLKNWKVSKIIWRR"
     gene            complement(25422..25871)
                     /locus_tag="NBI74_000618"
     CDS             complement(25422..25871)
                     /locus_tag="NBI74_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2919 domain-containing protein"
                     /protein_id="HCD1930994.1"
                     /translation="MKSTEFHPVHYDAHGRLRLPLLFWLVLLLQARTWVLFVIAGASR
                     EQGTALLNLFYPDHDNFWLGLIPGIPAVLAFLLSGRRASFPRIWHVLYFLLLLAQVVL
                     LCWQPWLWLNGESVSGIGLALVVADIVALIWLLTNRRLRACFNEEKE"
     gene            complement(25858..26283)
                     /locus_tag="NBI74_000619"
     CDS             complement(25858..26283)
                     /locus_tag="NBI74_000619"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311332.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family acetyltransferase"
                     /protein_id="HCD1930995.1"
                     /translation="MEIRVFRQEDFEEVITLWERCDLLSPWNDPEMDIERKMNHDVSL
                     FLVAEVNGEVVGTVMGGYDGHRGSAYYLGVHPEFRGRGIANALLNRLEKKLIARGCPK
                     IQINVPEDNDMVLGMYERLGYEHADVLSLGKRLIEDEEY"
     gene            26497..27366
                     /gene="amiA"
                     /locus_tag="NBI74_000620"
     CDS             26497..27366
                     /gene="amiA"
                     /locus_tag="NBI74_000620"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiA"
                     /protein_id="HCD1930996.1"
                     /translation="MSTFKPLKTLTSRRQVLKAGLAALTLSGMSQAIAKEEPLKTSNG
                     HSKPKAKKSGGRRVVVLDPGHGGIDTGAIGRNGSKEKHVVLAIAKNVRSILRNHGIDA
                     RLTRSGDTFIPLYDRVEIAHKHGADLFMSIHADGFTNPKAAGASVFALSNRGASSAMA
                     KYLSERENRADEVAGKKATDKDHLLQQVLFDLVQTDTIKNSLTLGSHILKKIKPVHKL
                     HSRNTEQAAFVVLKSPSVPSVLVETSFITNPEEERLLGTAAFRQKIATAIAEGVISYF
                     HWFDNQKAHSRKR"
     gene            27370..28269
                     /gene="hemF"
                     /locus_tag="NBI74_000621"
     CDS             27370..28269
                     /gene="hemF"
                     /locus_tag="NBI74_000621"
                     /EC_number="1.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-dependent coproporphyrinogen oxidase"
                     /protein_id="HCD1930997.1"
                     /translation="MKPDAHLVKQFLLNLQDTICQQLTAVDGAEFVEDSWQREAGGGG
                     RSRVLRNGGVFEQAGVNFSHVHGEAMPASATAHRPELAGRSFEAMGVSLVVHPHNPYV
                     PTSHANVRFFIAEKPGAEPVWWFGGGFDLTPFYGFEEDAIHWHRTARDLCLPFGEDVY
                     PRYKKWCDEYFYLKHRNEQRGIGGLFFDDLNTPDFDHCFAFMQAVGKGYTDAYLPIVE
                     RRKAMAYGERERNFQLYRRGRYVEFNLVWDRGTLFGLQTGGRTESILMSMPPLVRWEY
                     DYQPKDGSPEAALSEFIKVRDWV"
     gene            complement(28275..29327)
                     /gene="eutR"
                     /locus_tag="NBI74_000622"
     CDS             complement(28275..29327)
                     /gene="eutR"
                     /locus_tag="NBI74_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator EutR"
                     /protein_id="HCD1930998.1"
                     /translation="MKKTRTANLHHLYHEPLPENLKLTPKVEVDNVHQRQTTDVYEHA
                     LTITAWQQIYDQLHPGKFHGEFTEILLDDIQVFREYTGLALRQSCLVWPNSFWFGIPA
                     TRGELGFIGSQCLGSAEIATRPGGTEFELSTPDDYTILGVVLSEDVITRQANFLHNPD
                     RVLHMLRSQSALEVKEQHKAALWGFVQQALATFCENPENLHQPAVRKVLGDNLLMAMG
                     AMLEEAQPMMTAESISHQSYRRLLSRAREYVLENMSEPVTVLDLCNQLHVSRRTLQNA
                     FHAILGIGPNAWLKRIRLNAVRRELISPWSQSTTVKDAAMQWGFWHLGQFATDYQQLF
                     AEKPSLTLHQRMREWG"
     gene            complement(29373..29873)
                     /gene="eutK"
                     /locus_tag="NBI74_000623"
     CDS             complement(29373..29873)
                     /gene="eutK"
                     /locus_tag="NBI74_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311336.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutK"
                     /protein_id="HCD1930999.1"
                     /translation="MINALGLLEVDGMVAAIDAADAMLKAANVRLLSHEVLDPGRLTL
                     VVEGDLAACRAALDAGCAAAMRTGRVISRKEIGRPDDDTQWLVTGFNRQPKQPVKEPD
                     APVIVAESADELLALLTSVRQGMTAGEVAAHFGWPLEKARNALEQLFSAGTLRKRSSR
                     YRLKPH"
     gene            complement(29886..30545)
                     /gene="eutL"
                     /locus_tag="NBI74_000624"
     CDS             complement(29886..30545)
                     /gene="eutL"
                     /locus_tag="NBI74_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutL"
                     /protein_id="HCD1931000.1"
                     /translation="MPALDLIRPSVTAMRVIASVNAEFARELKLPPHIRSLGLISADS
                     DDVTYIAADEATKQAMVEVVYGRSLYAGAAHGPSPTAGEVLIMLGGPNPAEVRAGLDA
                     MVANIENGAAFQWANDAENTAFLAHVVSRTGSYLSSTAGITLGDPMAYLVAPPLEATY
                     GIDAALKSADVQLVTYVPPPSETNYSAAFLTGSQAACKAACNAFTDAVLEIARNPIQR
                     A"
     gene            complement(30555..31442)
                     /gene="eutC"
                     /locus_tag="NBI74_000625"
     CDS             complement(30555..31442)
                     /gene="eutC"
                     /locus_tag="NBI74_000625"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit beta"
                     /protein_id="HCD1931001.1"
                     /translation="MDQKQIEEIVRSVMASMGQAAPAPSEAKCATTTCAAPVTSESCA
                     LDLGSAEAKAWIGVENPHHADVLTELRRSTVARVCTGRAGPRPRTQALLRFLADHSRS
                     KDTVLKEVPEEWVKAQGLLEVRSEISDKNLYLTRPDMGRRLCAEAVEALKAQCVANPD
                     VQVVISDGLSTDAITVNYEEILPPLMAGLKQAGLKVGTPFFVRYGRVKIEDQIGEILG
                     AKVVILLVGERPGLGQSESLSCYAVYSPRMATTVEADRTCISNIHQGGTPPVEAAAVI
                     VDLAKRMLEQKASGINMTR"
     gene            complement(31463..32824)
                     /gene="eutB"
                     /locus_tag="NBI74_000626"
     CDS             complement(31463..32824)
                     /gene="eutB"
                     /locus_tag="NBI74_000626"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit alpha"
                     /protein_id="HCD1931002.1"
                     /translation="MKLKTTLFGNVYQFKDVKEVLAKANELRSGDVLAGVAAASSQER
                     VAAKQVLSEMTVADIRNNPVIAYEDDCVTRLIQDDVNETAYNQIKNWSISELREYVLS
                     DETSVDDIAFTRKGLTSEVVAAVAKICSNADLIYGAKKMPVIKKANTTIGIPGTFSAR
                     LQPNDTRDDVQSIAAQIYEGLSFGVGDAVIGVNPVTDDVENLSRVLDTIYGVIDKFNI
                     PTQGCVLAHVTTQIEAIRRGAPGGLIFQSICGSEKGLKEFGVELAMLDEARAVGAEFN
                     RIAGENCLYFETGQGSALSAGANFGADQVTMEARNYGLARHYDPFIVNTVVGFIGPEY
                     LYNDRQIIRAGLEDHFMGKLSGISMGCDCCYTNHADADQNLNENLMILLATAGCNYIM
                     GMPLGDDIMLNYQTTAFHDTATVRQLLNLRPSPEFERWLESMGIMANGRLTKRAGDPS
                     LFF"
     gene            complement(32836..34239)
                     /gene="eutA"
                     /locus_tag="NBI74_000627"
     CDS             complement(32836..34239)
                     /gene="eutA"
                     /locus_tag="NBI74_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase reactivating factor
                     EutA"
                     /protein_id="HCD1931003.1"
                     /translation="MNTRQLLSVGIDIGTTTTQVIFSHLELVNRAAVSQVPRYEFIKR
                     EISWQSPVFFTPVDKQGGLKEAELKTLILEQYQAAGIAPESVDSGAIIITGESAKTRN
                     ARPAVMALSRSLGDFVVASAGPHLESVIAGHGAGAQTLSEQRLCRVLNIDIGGGTANY
                     ALFDAGKISGTACLNVGGRLLETDSQGRVVYAHKPGQMIVDECFGPGTDARSLTGAQL
                     AQVTRRMAELIVEVIDGTLSPLAQALMQTGLLPAGVTPEIITLSGGVGECYRHQPADP
                     FCFADIGPLLATALHDHPRLREMNVQFPAQTVRATVIGAGAHTLSLSGSTIWLEGVQL
                     PLRNLPVAIPIDETDLVSAWQQALIQLDLCPKTDAYVLALPASLPVRYAAVLTVINAL
                     VDFVARFPNPHPLLVVAGQDFGKALGMLLRPQLQQLPLAVIDEVIVRAGDYIDIGTPL
                     FGGSVVPVTVKSLAFPS"
     gene            complement(34236..35462)
                     /gene="eutH"
                     /locus_tag="NBI74_000628"
     CDS             complement(34236..35462)
                     /gene="eutH"
                     /locus_tag="NBI74_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutH"
                     /protein_id="HCD1931004.1"
                     /translation="MGINEIIMYIMMFFMLIAAVDRILSQFGGSARFLGKFGKSIEGS
                     GGQFEEGFMAMGALGLAMVGMTALAPVLAHVLGPVIIPVYEMLGANPSMFAGTLLACD
                     MGGFFLAKELAGGDVAAWLYSGLILGSMMGPTIVFSIPVALGIIEPSDRRYLALGVLA
                     GIVTIPIGCIAGGLVAMYSGVQINGQPVEFTFALILMNMIPVLIVAVLVALGLKFIPE
                     KMINGFQIFAKFLVALITLGLAAAVVKFLLGWELIPGLDPIFMAPGDKPGEVMRAIEV
                     IGSISCVLLGAYPMVLLLTRWFEKPLMSVGKVLNMNNIAAAGMVATLANNIPMFGMMK
                     QMDTRGKVINCAFAVSAAFALGDHLGFAAANMNAMIFPMIVGKLIGGVTAIGVAMMLV
                     PKEDASAAKTEAEAQS"
     gene            complement(35679..36866)
                     /gene="eutG"
                     /locus_tag="NBI74_000629"
     CDS             complement(35679..36866)
                     /gene="eutG"
                     /locus_tag="NBI74_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization ethanol dehydrogenase
                     EutG"
                     /protein_id="HCD1931005.1"
                     /translation="MQNELQTALFQAFDTLNLQRVKTFSVPPVTLCGPGAVSSCGQQA
                     QTRGLKHLFVMADSFLHQAGMTAGLTRSLAVKGIAMTLWPCPVGEPCITDVCAAVAQL
                     RESGCDGVIAFGGGSVLDAAKAVALLVTNPDSTLAEMSETSVLQPRLPLIAIPTTAGT
                     GSETTNVTVIIDAVSGRKQVLAHASLMPDVAILDAVLTEGVPSHVTAMTGIDALTHAI
                     EAYSALNATPFTDSLAIGAIAMIGQSLPKAVGYGHDLVARESMLLASCMAGMAFSSAG
                     LGLCHAMAHQPGAALHIPHGLANAMLLPTVMEFNRMVCRDRFSQIGRALRTKKSDDRD
                     AINAVSELIAEVGINKRLADVGATTGHYGAWAQAAQEDICLRSNPRTASLEQIVGLYA
                     AAQ"
     gene            complement(36856..37692)
                     /gene="eutJ"
                     /locus_tag="NBI74_000630"
     CDS             complement(36856..37692)
                     /gene="eutJ"
                     /locus_tag="NBI74_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutJ"
                     /protein_id="HCD1931006.1"
                     /translation="MAHDEQWLTPRLQTAATLCNQTPAATESPLWLGVDLGTCDVVSM
                     VVDRDGQPVAVCLDWADVVRDGIVWDFFGAVTIVRRHLDTLEQQFGRRFSHAATSFPP
                     GTDPRISINVLESAGLEVSHVLDEPTAVADLLQLDNAGVVDIGGGTTGIAIVKKGKVT
                     YSADEATGGHHISLTLAGNRRISLEEAEQYKRGHGDEIWPAVKPVYEKMADIVARHIE
                     GQGITDLWLAGGSCMQPGVAALFCKQFPALQVHLPQHSLFMTPLAIASSGREKAEGLY
                     AK"
     gene            complement(37703..39106)
                     /locus_tag="NBI74_000631"
     CDS             complement(37703..39106)
                     /locus_tag="NBI74_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase EutE"
                     /protein_id="HCD1931007.1"
                     /translation="MNQQDIEQVVKAVLLKMQSSDTPPAAVHEMGVFASLDDAVAAAK
                     VAQQGLKSVAMRQLAIAAIREAGEKHARDLAELAVSETGMGRVEDKFAKNVAQARGTP
                     GVECLSPQVLTGDNGLTLIENAPWGVVASVTPSTNPAATVINNAISLIAAGNSVIFAP
                     HPAAKKVSQRAITLLNLAIVAAGGPENLLVTVTNPDIETAQRLFKFPGIGLLVVTGGE
                     AVVEAARKHTNKRLIAAGAGNPPVVVDETADLARAAQSIVKGASFDNNIICADEKVLI
                     VVDSVADELMRLMEGQHAVKLTAEQAQQLQSVLLKNIDERGKGTVSRDWVGRDAAKIA
                     AAIGLKVPEQTRLLFVETTAEHPFAVTELMMLVLPVVRVANVADAIALAVKLEGGCHH
                     TAAMHSRNIENMNQMANAIDTSIFVKNGPCIAGLGLGGEGWTTMTITTPTGEGVTSAR
                     TFVRLRRCVLVDAFRIV"
     gene            complement(39118..39405)
                     /gene="eutN"
                     /locus_tag="NBI74_000632"
     CDS             complement(39118..39405)
                     /gene="eutN"
                     /locus_tag="NBI74_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutN"
                     /protein_id="HCD1931008.1"
                     /translation="MKLAVVTGQIVCTVRHHGLAHDKLLMVEMIDPQGNPDGQCAVAI
                     DNIGAGTGEWVLLVSGSSARQAHKSETSPVDLCVIGIVDEVVSGGQVIFHK"
     gene            complement(39512..39805)
                     /gene="eutM"
                     /locus_tag="NBI74_000633"
     CDS             complement(39512..39805)
                     /gene="eutM"
                     /locus_tag="NBI74_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutM"
                     /protein_id="HCD1931009.1"
                     /translation="MEALGMIETRGLVALIEASDAMVKAARVKLVGVKQIGGGLCTAM
                     VRGDVAACKAATDAGAAAAQRIGELVSVHVIPRPHGDLEEVFPIGLKGDSSNL"
     gene            complement(39844..40860)
                     /gene="pta"
                     /locus_tag="NBI74_000634"
     CDS             complement(39844..40860)
                     /gene="pta"
                     /locus_tag="NBI74_000634"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HCD1931010.1"
                     /translation="MIIKRCRELALRAPARVVFPDALDQRVLKAAQYLHQQGLATPIL
                     VANPFELRQFALSHGVAMDGLQVIDPHGNLAMREEFAHRWLARAGEKTPPDALEKLTD
                     PLMFAAAMVSAGKADVCIAGNLSSTANVLRAGLRIIGLQPGCKTLSSIFLMLPQYSGP
                     ALGFADCSVVPQPTAAQLADIALASAETWRAITGEEPRVAMLSFSSNGSARHPCVANV
                     QQATEIVRERAPKLVVDGELQFDAAFVPEVAAQKAPASPLQGKANVMVFPSLEAGNIG
                     YKIAQRLGGYRAVGPLIQGLAAPMHDLSRGCSVQEIIELALVAAVPRQTEVNRESSLQ
                     TLVE"
     gene            complement(40857..41660)
                     /gene="eutT"
                     /locus_tag="NBI74_000635"
     CDS             complement(40857..41660)
                     /gene="eutT"
                     /locus_tag="NBI74_000635"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization cob(I)yrinic acid
                     a,c-diamide adenosyltransferase EutT"
                     /protein_id="HCD1931011.1"
                     /translation="MKDFITEAWLRANHTLSEGAEIHLPADSRLTPSARELLESRHLR
                     IKFIDEQGRLFVDDEQQQPQPVHGLTSSDEHPQACCELCRQPVAKKPDTLTHLSAEKM
                     VAKSDPRLGFRAVLDSTIALAVWLQIELAEPWQPWLADIRSRLGNIMRADALGEPLGE
                     QAIVGLSDEDLHRLSHQPLRYLDHDHLVPEASHGRDAALLNLLRTKVRETETVAAQVF
                     ITRSFEVLRPDILQALNRLSSTVYVMMILSVTKQPLTVKQIQQRLGETQ"
     gene            complement(41657..42358)
                     /gene="eutQ"
                     /locus_tag="NBI74_000636"
     CDS             complement(41657..42358)
                     /gene="eutQ"
                     /locus_tag="NBI74_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutQ"
                     /protein_id="HCD1931012.1"
                     /translation="MKKLITANDIREAHARGELAMSVVLRASIITPEAREVADLLGFT
                     ITECDESIPVTASVPASASADKTESQRIRETIIAQLPEGQFTESLVAQLMEKVMKEKQ
                     SLEQGALQPSFKSVTGKGGIKVIDGSSVKFGRFDGAEPHCVGLTDLVTGDDGSSMAAG
                     FMQWENAFFPWTLNYDEIDMVLEGELHVRHEGETMIAKAGDVMFIPKGSSIEFGTTSS
                     VKFLYVAWPANWQSL"
     gene            complement(42333..42812)
                     /gene="eutP"
                     /locus_tag="NBI74_000637"
     CDS             complement(42333..42812)
                     /gene="eutP"
                     /locus_tag="NBI74_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutP"
                     /protein_id="HCD1931013.1"
                     /translation="MKRIAFVGTVGAGKTTLFNALQGNYTLARKTQAVEFNDNGDIDT
                     PGEYFSHPRWYHALITTLQDVDMLIYVHGANDPESRLPAGLLDIGVSKRQIAVISKTD
                     MPDADVAATRKLLLETGFEEPIFELNSHDPQSVQQLVDYLASLTKQEEAGEKTHHSE"
     gene            complement(42825..43160)
                     /gene="eutS"
                     /locus_tag="NBI74_000638"
     CDS             complement(42825..43160)
                     /gene="eutS"
                     /locus_tag="NBI74_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutS"
                     /protein_id="HCD1931014.1"
                     /translation="MDKERIIQEFVPGKQVTLAHLIAHPGEELAKKIGVPDAGAIGIM
                     TLTPGETAMIAGDLALKAADVHIGFLDRFSGALVIYGSVGAVEEALSQTVSGLGRLLN
                     YTLCEMTKS"
     gene            complement(43453..45732)
                     /gene="maeB"
                     /locus_tag="NBI74_000639"
     CDS             complement(43453..45732)
                     /gene="maeB"
                     /locus_tag="NBI74_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxaloacetate-decarboxylating
                     malate dehydrogenase"
                     /protein_id="HCD1931015.1"
                     /translation="MDDQLKQSALDFHEFPVPGKIQVSPTKPLATQRDLALAYSPGVA
                     APCLEIEKDPLKAYKYTARGNLVAVISNGTAVLGLGNIGALAGKPVMEGKGVLFKKFA
                     GIDVFDIEVDELDPDKFIEVVAALEPTFGGINLEDIKAPECFYIEQKLRERMNIPVFH
                     DDQHGTAIISTAAILNGLRVVEKNISDVRMVVSGAGAAAIACMNLLVALGLQKHNIVV
                     CDSKGVIYQGREPNMAETKAAYAVVDDGKRTLDDVIEGADIFLGCSGPKVLTQEMVKK
                     MARAPMILALANPEPEILPPLAKEVRPDAIICTGRSDYPNQVNNVLCFPFIFRGALDV
                     GATAINEEMKLAAVRAIAELAHAEQSEVVASAYGDQDLSFGPEYIIPKPFDPRLIVKI
                     APAVAKAAMESGVATRPIADFDVYIDKLTEFVYKTNLFMKPIFSQARKAPKRVVLPEG
                     EEARVLHATQELVTLGLAKPILIGRPNVIEMRIQKLGLQIKAGVDFEIVNNESDPRFK
                     EYWTEYFQIMKRRGVTQEQAQRALISNPTVIGAIMVQRGEADAMICGTVGDYHEHFSV
                     VKNVFGYRDGVHTAGAMNALLLPSGNTFIADTYVNDEPDAEELAEITLMAAETVRRFG
                     IEPRVALLSHSNFGSSDCPSSSKMRQALELVRERAPELMIDGEMHGDAALVEAIRNDR
                     MPDSPLKGSANILVMPNMEAARISYNLLRVSSSEGVTVGPVLMGVAKPVHVLTPIASV
                     RRIVNMVALAVVEAQTQPL"
     gene            46021..46971
                     /gene="tal"
                     /locus_tag="NBI74_000640"
     CDS             46021..46971
                     /gene="tal"
                     /locus_tag="NBI74_000640"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HCD1931016.1"
                     /translation="MNELDGIKQFTTVVADSGDIESIRHYHPQDATTNPSLLLKAAGL
                     SQYEHLIDDAIAWGKKNGKTQEQQVVAACDKLAVNFGAEILKIVPGRVSTEVDARLSF
                     DKEKSIEKARHLVDLYQQQGVEKSRILIKLASTWEGIRAAEELEKEGINCNLTLLFSF
                     AQARACAEAGVFLISPFVGRIYDWYQARKPMDPYVVEEDPGVKSVRNIYDYYKQHHYE
                     TIVMGASFRRTEQILALTGCDRLTIAPNLLKELQEKVSPVVRKLIPPSQTFPRPAPMS
                     EAEFRWEHNQDAMAVEKLSEGIRLFAVDQRKLEDLLAAKL"
     gene            46991..48994
                     /gene="tkt"
                     /locus_tag="NBI74_000641"
     CDS             46991..48994
                     /gene="tkt"
                     /locus_tag="NBI74_000641"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HCD1931017.1"
                     /translation="MSRKDLANAIRALSMDAVQKANSGHPGAPMGMADIAEVLWNDFL
                     KHNPTDPTWYDRDRFILSNGHASMLLYSLLHLTGYDLPLEELKNFRQLHSKTPGHPEI
                     GYTPGVETTTGPLGQGLANAVGLAIAERTLAAQFNQPDHEIVDHFTYVFMGDGCLMEG
                     ISHEVCSLAGTLGLGKLIGFYDHNGISIDGETEGWFTDDTAKRFEAYHWHVVHEIDGH
                     DPQAVKEAILEAQSVKDKPSLIICRTVIGFGSPNKAGKEEAHGAPLGEEEVALARQKL
                     GWHHPPFEIPKEIYHAWDAREKGEKAQQSWNEKFAAYKKAHPQLAEEFTRRMSGGLPK
                     DWEKTTQKYINELQANPAKIATRKASQNTLNAYGPMLPELLGGSADLAPSNLTIWKGS
                     VSLKEDPAGNYIHYGVREFGMTAIANGIAHHGGFVPYTATFLMFVEYARNAARMAALM
                     KARQIMVYTHDSIGLGEDGPTHQAVEQLASLRLTPNFSTWRPCDQVEAAVGWKLAVER
                     HNGPTALILSRQNLAQVERTPDQVKEIARGGYVLKDSGGKPDIILIATGSEMEITLQA
                     AEKLAGEGRNVRVVSLPSTDIFDAQDEKYRESVLPSNVSARVAVEAGIADYWYKYVGL
                     KGAIVGMTGYGESAPADKLFPFFGFTAENIVAKAHKVLGVKGA"
     gene            complement(49071..50114)
                     /locus_tag="NBI74_000642"
     CDS             complement(49071..50114)
                     /locus_tag="NBI74_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1176 domain-containing protein"
                     /protein_id="HCD1931018.1"
                     /translation="MRYRIFLLFFFALLPMSLVWAAPAQRAFSDWQVTCNNQNFCVAR
                     NTGDHNGLVMTLSRSAGAHTDAVLRIERGGLKSPDASEGEIAPRLLLDGEPLALSGDK
                     WRISPWLLVTDDTATITAFLQMIQEGRAITLRDGNQTISLSGLKAALLFIDAQQKRVG
                     SETAWIKKGDEPPLSVPPAPALKEVAVVNPTPTPLSLEERNDLLDYGNWRMNGLRCSL
                     DPLRREVNVTALTDDKALMMISCEAGAYNTIDLAWIVSRKKPQASRPVRLRLPFNNGQ
                     ETNELELMNATFDEKSRELVTLAKGRGLSDCGIQARWRFDGQRFRLVRYAAEPTCDNW
                     HGPDAWPTLWITR"
     gene            complement(50240..50815)
                     /gene="nudK"
                     /locus_tag="NBI74_000643"
     CDS             complement(50240..50815)
                     /gene="nudK"
                     /locus_tag="NBI74_000643"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose pyrophosphatase NudK"
                     /protein_id="HCD1931019.1"
                     /translation="MTQQITLVKDKILSDNYFTLHNITYDLTRKDGEVIRHKREVYDR
                     GNGATILLYNAKKKTVVLIRQFRVATWVNGNESGQLIETCAGLLDNDEPEVCIRKEAI
                     EETGYEVGEVRKLFELYMSPGGVTELIHFFIAEYSDSQRANAGGGVEDEDIEVLELPF
                     SQALEMIKTGEIRDGKTVLLLNYLQMSHLMD"
     gene            complement(50883..52862)
                     /gene="aegA"
                     /locus_tag="NBI74_000644"
     CDS             complement(50883..52862)
                     /gene="aegA"
                     /locus_tag="NBI74_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent uric acid utilization protein
                     AegA"
                     /protein_id="HCD1931020.1"
                     /translation="MNRFIMANSQQCLGCHACEIACVMAHNDEQHVLSQHHFHPRITV
                     IKHQQQRSAVTCHHCEDAPCARSCPNGAISHVDDSIQVNQQKCIGCKSCVVACPFGTM
                     QIVLTPVAAGKVKATAHKCDLCAGRENGPACVENCPADALQLVTDAALSGMAKSRRLR
                     TARQEHQPWHASTAAQEMPVMSKVEQMQATPARGEPDKLAIEARKTGFDEIYLPFRAD
                     QAQREASRCLKCGEHSVCEWTCPLHNHIPQWIELVKAGNIDAAVELSHQTNTLPEITG
                     RVCPQDRLCEGACTIRDEHGAVTIGNIERYISDQALAKGWRPDLSHVTKVDKRVAIIG
                     AGPAGLACADVLTRNGVAVTVYDRHPEIGGLLTFGIPSFKLDKSLLARRREIFSAMGI
                     HFELNCEVGKDVSLNSLLEQYDAVFVGVGTYRSMKAGLPNEDAPGVYDALPFLIANTK
                     QVMGLEELPEEPFINTAGLNVVVLGGGDTAMDCVRTALRHGASNVTCAYRRDEANMPG
                     SKKEVKNAREEGANFEFNVQPVALELNEQGHVCGIRFLRTRLGEPDAQGRRRPVPVEG
                     SEFVMPADAVIMAFGFNPHGMPWLESHGVTVDKWGRIIADVESQYRYQTTNPKIFAGG
                     DAVRGADLVVTAMAEGRHAAQGIIDWLGVKSVKSH"
     gene            53068..54768
                     /gene="narQ"
                     /locus_tag="NBI74_000645"
     CDS             53068..54768
                     /gene="narQ"
                     /locus_tag="NBI74_000645"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarQ"
                     /protein_id="HCD1931021.1"
                     /translation="MIVKRPVSASLARAFFYIVLLSILSTGIALLTLASSLRDAEAIN
                     IAGSLRMQSYRLGYDLQSGSPQLNAHRQLFQQALHSPVLTNLNVWYVPEAVKTRYAHL
                     NANWLEMNNRLSKGDLPWYQANINNYVNQIDLFVLALQHYAERKMLLVVAISLAGGIG
                     IFTLVFFTLRRIRHQVVAPLNQLVTASQRIEHGQFDSPPLDTSLPNELGLLAKTFNQM
                     SSELHKLYRSLEASVEEKTRDLHEAKRRLEVLYQCSQALNTSQIDVHCFRHILQIVRD
                     NEAAEYLELNVGDNWRISEGQPNPELPMQILPVTMQETVYGELHWQNSHVSSSEPLLN
                     SVSSMLGRGLYFNQAQKHFQQLLLMEERATIARELHDSLAQVLSYLRIQLTLLKRSIP
                     EDNATAQSIMADFSQALNDAYRQLRELLTTFRLTLQQADLPSALREMLDTLQNQTSAK
                     LTLDCRLPTLALDAQMQVHLLQIIREAVLNAMKHANASEIAVSCVTAPDGNHTVYIRD
                     NGIGIGEPKEPEGHYGLNIMRERAERLGGTLTFSQPSGGGTLVSISFRSAEGEESQLM
                     "
     gene            54932..58045
                     /gene="acrD"
                     /locus_tag="NBI74_000646"
     CDS             54932..58045
                     /gene="acrD"
                     /locus_tag="NBI74_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease AcrD"
                     /protein_id="HCD1931022.1"
                     /translation="MANFFIDRPIFAWVLAILLCLTGTLAIFSLPVEQYPDLAPPNVR
                     VTANYPGASAQTLENTVTQVIEQNMTGLDNLMYMSSQSSGTGQASVTLSFKAGTDPDE
                     AVQQVQNQLQSAMRKLPQAVQNQGVTVRKTGDTNILTIAFVSTDGSMDKQDIADYVAS
                     NIQDPLSRVNGVGDIDAYGSQYSMRIWLDPAKLNSFQMTAKDVTDAIESQNAQIAVGQ
                     LGGTPSVDKQALNATINAQSLLQTPEQFRDITLRVNQDGSEVRLGDVATVEMGAEKYD
                     YLSRFNGKPASGLGVKLASGANEMATAELVLNRLDELAQYFPHGLEYKVAYETTSFVK
                     ASIEDVVKTLLEAIALVFLVMYLFLQNFRATLIPTIAVPVVLMGTFSVLYAFGYSVNT
                     LTMFAMVLAIGLLVDDAIVVVENVERIMSEEGLTPREATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGTTGAIYRQFSITIVAAMVLSVLVAMILTPALCATLLKPLKKGEHHGQ
                     KGFFAWFNQMFNRNAERYEKGVAKILHRSLRWIVIYVLLLGGMVFLFLRLPTSFLPLE
                     DRGMFTTSVQLPSGSTQQQTLKVVEQIEKYYFTHEKDNIMSVFATVGSGPGGNGQNVA
                     RMFIRLKDWSERDSKTGTSFAIIERATKAFNKIKEARVIASSPPAISGLGSSAGFDME
                     LQDHAGAGHDALMAARNQLLALAAENPELTRVRHNGLDDSPQLQIDIDQRKAQALGVA
                     IDDINDTLQTAWGSSYVNDFMDRGRVKKVYVQAAAPYRMLPDDINLWYVRNKDGGMVP
                     FSAFATSRWETGSPRLERYNGYSAVEIVGEAAPGVSTGTAMDIMESLVKQLPNGFGLE
                     WTAMSYQERLSGAQAPALYAISLLVVFLCLAALYESWSVPFSVMLVVPLGVIGALLAT
                     WMRGLENDVYFQVGLLTVIGLSAKNAILIVEFANEMNQKGHDLFEATLHACRQRLRPI
                     LMTSLAFIFGVLPMATSTGAGSGGQHAVGTGVMGGMISATILAIYFVPLFFVLVRRRF
                     PLKPRPE"
     gene            complement(58144..58203)
                     /gene="ypfM"
                     /locus_tag="NBI74_000647"
     CDS             complement(58144..58203)
                     /gene="ypfM"
                     /locus_tag="NBI74_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YpfM"
                     /protein_id="HCD1931023.1"
                     /translation="MIERELGNWKDFIEVMLRK"
BASE COUNT        13729 a        16102 c        14902 g        13698 t
ORIGIN      
        1 cataaagtat ttcaccacca gatatccgac atacataaac acgatggata atatacccgg
       61 caatacattt ggaacaggaa taggtaatcg cacgattgta aacaaagcac caatgccaaa
      121 accaacgcca gaagaaaata aaatatcttt cactaattaa cctttatcat aaaagcagcc
      181 ccaaggaaca gagctgcgaa tccttttaat gtgtaactga acgatgcgtt atcttttcag
      241 ggtcatggtt atatttaaac ccaaagaaaa atatcactgc gaggaaaaga gcataccctg
      301 caaacatcag ccaaatggta tgccaatctt ttacgccatc caccgagaag taatccactg
      361 ccataccgct cagaatcgag ccaacccatg cgccgacgcc atttaccatc gtcataaaaa
      421 ggccctgggc gctggcacga atgctggaat caacttcctg ttcgacaaag accgaaccag
      481 aaatattgaa gaaatcgaat gcacaaccgt aaacaatcat cgacagcaac agcaaaataa
      541 acccggttgg tgatggatcg ccataggcaa agaagccaaa gcgcagcgtc cacgccacca
      601 tactcatcag catgacggtt ttaataccga aacgcttcaa aaagaacggt atcgtcagaa
      661 tgaaaccgac ttcagccatc tgtgaaactg acagtaaaat ggacgggtat ttcaccacga
      721 agctatcagc aaactcagga ttacgggcga aatcatgcag gaacgggttg ccaaaaacgt
      781 tggtaatttg cagcaccgca cccagcatca tggcgaagag gaaaaagata gccatgcgtg
      841 gatttttaaa cagcacgaag gcatccagac ccagcttgct ggcaagcgat gtagtcgctt
      901 ttttctccgc aaccggaatc ttcggcaaag tcagcgcata agccgacagc agcaatgacg
      961 caccggacgc gatatacagt tgcagactac tcaattccag atgcagcaag cttactgccc
     1021 acatggcgac aatgaacccc accgtaccaa aaacgcgaat gggcgggaaa gcggtcaccg
     1081 ggtcaagccc tgcctgggca agacaggaat aagagacgct gttcgataac gcaatagtcg
     1141 gcataaacgc catcgcattg actaacatca cccaaaacat catatccgga tcagttacgg
     1201 atgccgcata aaaaagtacg cccgcacaca ccaggtgaca cagcatgtat gcacgttctg
     1261 cacgcagcca tttgtctgcg atgatcccca ttataccagg cataataatc gcggcgatcc
     1321 ctttggaact gtaaaccatg ccaacattag cgccggtgaa atgaagggta ttaatcatgt
     1381 aagaaccgag ggtaaccagc cagctccccc agataaaata ttgcaaaaag gacattacct
     1441 ttaagcgcaa agcgatgctc ataatcgttt ccttgtcgca tcatatggcc tcactcagta
     1501 aggccgttta ttgttgtttt actcaggcaa ttttgcgcag aaagccgcaa ataagattaa
     1561 taaagttttg ctttgagagt tccgctgctg ccagcgtttg ggcatgagaa agcttcacgt
     1621 cactcagacc ttccgccatg ttggtaatcg cagagaccgc aacgacttta agttcgcaat
     1681 ggcgagctga aataacctca ggcaccacag acataccaac aacatcccca ccaataattt
     1741 gcatcatgcg aatttccgcc gcagtctcga aattcggccc cggatacgag acgaacacgc
     1801 cctccgtcag agggaccccc tcttctttcg ccactttttg taacagtgcg cggtattccg
     1861 catcgtaggc attcgccagc gagaagaagc gctcaccaaa acgttcatca ttaagaccca
     1921 ccatcggcgt tcccggcatg gtgttgatgt gatctttcaa tgcgaccaga ctgccggccc
     1981 ccacttcagg gcgcagtgag ccagccgcat tggtgcagaa cagcaactcg cagcccagca
     2041 acttaaaggt acggattgca tccgtcatga tggtcatccc acgaccttcg tagaaatgtc
     2101 cgcgaccttt catgcacacc actggcaccc cctggagata ccccagcacc agctcacccg
     2161 catgaccgtg tacggtactt accgggaacc caggcagctt ttcgtaggaa attgcgaccg
     2221 cgttctcaat ctgatcggcc agcgcgccca gcccggaacc taaaataaag gccactcgtg
     2281 gcgtgaaatc aggtttataa gtcttgataa tatctacgca atacagtggg ttatgagaaa
     2341 actgagcctg atacatatat atcctttttt tgtagggggg aatctaacgc ctttttataa
     2401 cgagattgcc ctacctcata ccaatacact tttttcatgt atccgatagt tattcgatat
     2461 cgatatattt atattttttc actattaata ggctgaaaaa actgtattgg tactctcctt
     2521 tcccttgcat taacaatatg ccgcttttaa tcaacctttt tattaagcaa tcaactaata
     2581 acgatcggca tagggaatta tgctatgaaa aaacatcttt ttactctgac actctcttct
     2641 gtattggcga taccagcagt atcccatgca gaatttaaag gcggttttgc tgatataggt
     2701 attcattatc ttgactggac tagcagaacc actgagaaat cgtcgaccaa atcacacaaa
     2761 gatgattttg gatatctgga actggaaggc ggcgctaact ttagttgggg agaaatgtac
     2821 gggttcttcg actgggaaaa cttttataat gatcgtcatg ataaaccggg tagcgagcag
     2881 cgttatacat ttaaaaatac caaccgtatt tacctgggtg acaccggatt taatctctac
     2941 ttgcatgcgt atggcaccta cggttctgcg aatcgtgtga atttccatga cgatatgttc
     3001 ctttacggga tcggctacaa tttcaccggc agcggttggt ggttcaaacc gttctttgca
     3061 aaacgctata cagaccaaac ctattatacc ggcgataacg gttatgtcgc cggttgggtt
     3121 gcaggttaca gctttatgct gggcagtgag aaattcaccc tcaccaactg gaacgagtac
     3181 gagtttgatc gtgatgccac ctacgcggca ggtaatggcg gcaaagaagg tctgaacggt
     3241 gccgtcgcac tttggtggaa tgcaacttca cacatcacca cagggattca gtatcgttat
     3301 gcggatgaca aactgggcga agatttttac caggatgcga taatctactc catcaaattt
     3361 aatttctgat tgattactgc cggagctccc ggcagtatga attcactcta tatctgatac
     3421 aacggatccc cttcccgccc agccattagc gcattgccct ggtctcgtac tacgtcccat
     3481 aacgcttcag cggcggtgga aagtgaacgg ttcttcctgc gcaccagcat caactggcgt
     3541 tcaaccactg gcgtaatgcg ctttaccacc agtgggctac cttcaggcaa tggtagcgcc
     3601 agtgcgggaa gaatactgat gccgatgcct gcggctacca tcgggaacag cgtcgccgga
     3661 tgtccaatct cctgcacaat attcgcctga ataccgttgc gcgccagcgc tgcgtcaatc
     3721 agcggtcggc tgcctgacgc gtaatcctgc aacaccaatt tcgctccctg taatgcctgc
     3781 caggggacat aatcttccac ggccaaagcg ctatcgcgat ggcaaagcag aaagaaaggc
     3841 tcggaaagaa tcgcttcaca ttgcagatcg cccactgggc caggatcgat gacgatgcca
     3901 aaatcgacat ctccctggcg aatactttcc atcacccact gctgcggacg atcgtgcagg
     3961 acaaactgaa tatctggata gcggcgatgg ctttcagcaa tgcattgcgg aataagatgc
     4021 gcggaaatgg tctggctggc agcgacccga acttttccgc tcagttgttg ccccatacgc
     4081 ccggtatcgc gcaacgtgct gttaagttca tccagcagtc gctcaagacg caaagccaac
     4141 tgctgccctg catctgtaag caccacttca cgcgtggttc tgtccagcag gcgaacacca
     4201 gtatgatttt ccagctcctt cacactgtga ctcactgccg actggctcag gccgatacgc
     4261 tctcctgcac gactaaaact tttctcctgc gctactgtga cgaaaacctt taattgcttt
     4321 aaagaataat tcatctgttt ttttcatgaa tggatgcaat aaatcaattt tatttctcaa
     4381 actggaagac gcacaataga accatcgatc atctggagtc tttatgaaac tttttcgtat
     4441 cctcgatcct ttcaccttaa ccctgatcac ggtggtgttg ctggcctctt tctttccggc
     4501 cagaggcgat ttcgtcccct tctttgaaaa tctgaccacc gcagcgatag ccctgctgtt
     4561 ttttatgcac ggcgcgaagt tatcgcgtga ggcgattatt gctggcggtg gccactggcg
     4621 actgcatttg tgggtaatgt gcagcacctt cgtgctgttt ccgattctgg gcgtgctgtt
     4681 tgcctggtgg aaaccggtaa atgtcgaccc gatgctctac tccggttttc tctacttgtg
     4741 cattctcccc gcgaccgtgc agtctgcaat cgccttcacg tcaatggcgg gcggtaacgt
     4801 cgcggcggcg gtttgttctg cgtcagcatc cagcctgctg gggattttcc tttcaccatt
     4861 gctggttggt ctggtgatga atgttcacgg tgcagggggc agccttgagc aggtcggtaa
     4921 aattatgctg caactgttgc tgccatttgt gttggggcat ctttcccggc cgtggattgg
     4981 tgactgggtg tcgcgtaata aaaaatggat tgcgaaaact gaccagacgt ccattctgtt
     5041 ggtagtttat acggcattca gcgaagccgt cgttaacggt atctggcaca aagttggctg
     5101 gggatcattg ctgtttatcg tggtggtcag ttgcgttctt ctggctatcg tgattgtagt
     5161 taacgtcttt atggcacgcc gactgggctt caataaggca gatgaaatta ctatcgtctt
     5221 ttgtggttcg aaaaagagtc tggcaaatgg catcccgatg gcaaacattc tgttccccac
     5281 atcggtgatc ggtatgatgg tgctgcccct gatgattttc catcagatcc aattgatggt
     5341 ctgtgcggtg ctggcgcgtc gatacaaacg ccagaccgaa cagttacagg cgcagcagga
     5401 aagcagcgcc gataaagctt aaagcggacg tttcaggggc tggaccagtt gcgtcagccc
     5461 ctcgatttta atcagcagcg tgatttgcat tagctcaccg agcttccctg ccgggaactc
     5521 atctttgcgg gcaaaccaca gcagatactc ctccggcagg tcgattaagc gacgcccttt
     5581 gtacttgcca aacggcatta tcgtattggc tatttcaatc agctgctctt tttccatctc
     5641 agctacccag caaacgcagc atttccgttt cgtcgatgac ttcaataccc agttcctgcg
     5701 ccttcgccag tttagatcct gcagcttcac ccgctatcac cagatcggtt ttcttcgaca
     5761 cgctgcccgc gactttcgcc cccagttcga ccagtcgagc tttagcgtca tcacgcgaca
     5821 tctggcttaa gctgcccgta agcaccacgg ttttaccagc aaacggactg tcaatctctt
     5881 ccgcattgat aacgatcggc tcaggccagt gaacaccttc cgccaacagc tcgctgatga
     5941 cattgcggtt gctttcttcg gcaaagaagt tgtgaacgtg ggaggcaacg acaattccaa
     6001 catcaggcac cttttgcagc tcttcaatcg acgcggcttc cagcgcttcc agcgtgccga
     6061 aatatgccgc cagacctgct gcggtagcct cgccgacttc acggatgcca agtgcataga
     6121 ggaagcgggc aaaggtggtt tctttcgctt tttccagcgc gttaaccacg ttttgtgccg
     6181 atttcggccc catacgctcc agtccggtca gtttgcctgc ggtgagtttg aacagatccg
     6241 ccggagtgtg gacatattct ttttcaacca gttgatcgat gattttgtcg cccattccgt
     6301 caacatccat cgcacggcgg gaaacaaagt gtttcagaga ctctttacgc tgcgcaccgc
     6361 aaatcaggcc accggtacag cgggcaaccg cttcaccttc cacacgctcg acgtcagaac
     6421 cacataccgg acaatgcgtc gggaatacaa cctcacgggt atcttccggg cgttcagaaa
     6481 gcacgacgtt aaccacctgc gggatcacgt cgccagcgcg gcgaataacc actttatcgc
     6541 caatgcgtaa accaagacgt tcgatttcgt cagcattatg cagtgtagcg ttactcacca
     6601 acacgccagc aacatggaca ggttccagac gcgcaacagg cgtaatcgcg ccagtacgcc
     6661 caacctgaaa ctcgacgtca cgcacaaagg tcatctgttc ctgcgccggg aatttaaacg
     6721 ctaccgccca gcgcggggca cgcgcgacaa agccaagctg ctcctgctgt gccagtgagt
     6781 tgaccttaat caccacgccg tcgatatcaa agcccagcgt cggacggtct ttttctactt
     6841 tgtgataaaa cgccagcact tcttccgccg attcacaaag tgttacccga tcactgacag
     6901 gtaatcccca tgctttaaat tgcattaagc gtcccaaatg ggtatccggc aattcaccgc
     6961 cttccagaac accaacgccg tagcagaaga aagtgagcgg tcgcttcgct gtaatacgcg
     7021 gatcaagctg acgcagtgaa ccagctgccg cattacgtgg gttagcaaac actttcccgc
     7081 ccgtgcgtcg cgcatcttcg ttaatctttt cgaaccccgc ctgcggcagg aacacttcac
     7141 cacgcacttc cagacgcgcc gggatattct caccgtgtag cttcagcgga atggcgcgaa
     7201 tagtacgcac attagacgtg atatcttccc cggtagtgcc atcgccacga gtcgcggcac
     7261 tgactaaaac gccattttca tacagaatac tgacggcaag accatccagc ttcagctcac
     7321 agcaccaggt gactttctcg ttgcttttca gacggtcctg cacacgtttg ttgaaagcaa
     7381 gaaagctttc ttcatcaaaa acgttatcca gcgacagcat tggcacttca tggcggatct
     7441 ggctgaaagc cgccagcggc gctgcaccta cacgttgggt aggcgaatca ggcgtaatca
     7501 gttctggatg tttggtttcc agctcgcgca gttcgcgcat cagcctgtcg tattcagcgt
     7561 cgggaatttc cggcgcatcc atcacatgat aaagatattc atgatggcga agcgtcgttc
     7621 gcagttctgt cagttgttgt tcgattgatt ccatatcgca ccatcaatgc taaaaacccc
     7681 cgacaagcgg gggttcgaaa aggagttaat ttgccttaag tgtatcaggc gttggcgtct
     7741 ttgacttcgc ggatgatgtc ctggtactcg cgcaatttct gcggagtcat catacggcgc
     7801 tgatcgtcaa gcactacacc acccacttca tcggcaatat gctgcgcaga ttgcagcatc
     7861 agcttgaagt tctgcagctc gtcaccgtaa gacggtacct gcataaagat agtgacaccc
     7921 ggagtagtga aatccttcat ttcaggatca aaggttcccg gtttcaccat attcgccagg
     7981 ctgaataacg ccgggccgct gccatccggg ctaagatgac gatggtaaat gttcatatca
     8041 ccaaaaatga agcccgcttg ttgaatgctg ttaagaagca gttcaccgtt taactcgcta
     8101 ccgtgatgcg ccgcgacgtt catgataatc accgcttctt tgcgcttcgg tttatccata
     8161 actggagccg gttccgcaac aggctcaggc tgtggtgccg ctacgggttc tgcaggctgg
     8221 aaagcctgtt gtgccggttg cggtgctgaa tgcactggct gcggcgctga cgcgacctgt
     8281 ggcgaaactg gctgctgcaa cggctgttca ggctgcggct gataggcagg ctgctgcacg
     8341 ggttgctgca ccggctgcgt tggacgcgga gcatgttgcg gcggtacctg cgcttcaggc
     8401 ggctgctgga ccggttgacg cggctgcgca gacgcataag gcggttggta ctggtgttgc
     8461 ggcgacggac gagcaacctc atgctcctgg gcgttagccg gggcatgatt cacgcggtga
     8521 acacgaacct caccaacgcc ctcatcatct tcgacatcct cgtcataaga atcgtcgtca
     8581 cgttttgact tcattcgttt taatggccga tcgcggaaca tagaagatcg ttctttacgg
     8641 ctggtccaga aaccatgtac cagtaaagcg attatggcga tcgcgccaac aatgattaat
     8701 atcagacgca aatcctgcat cattatattc tctgttgttc taacaccttg ccaccacggc
     8761 aaacatttac tcactaagag tatttgccga ttacctcaag tgcaagtgca ctattaactt
     8821 tcacagcaca aagatagatg aaatcgtgct ttttgctgtt ttttcgaaca tatcctaact
     8881 gtccattgcg caattacccg gtaaaatacg cagaattttc ctgggattgg tcaaaaggag
     8941 ctcatcctga ttatggtttc atcattcaca tctgccccac gcagcggttt ttactatttt
     9001 gcgcaaggct ggaagctcgt ctcgcaacct gggattcggc gtttcgttat tttaccgctg
     9061 ctggtcaata ttttgttgat ggggggcgca ttctggtggc tctttacgca actcgatgtc
     9121 tggatcccga ctctcatgag ttatgttccg gactggttac aatggctgag ctatctgttg
     9181 tggcccctgg ctgtcatctc tgtgctgtta gtgtttggct atttcttctc aacgattgct
     9241 aactggattg ctgctccgtt caacggttta ttggctgaac aactggaagc aagactgact
     9301 ggcgctacac cgccagatac cggaattttc ggtatcatga aagatgtgcc gcgaatcatg
     9361 aaacgcgaat ggcaaaaatt tgcctggtat ctgccgcgcg caattgtatt gctaattctt
     9421 tacttcatcc ctggcattgg gcaaaccgtc gcgccggtac tgtggttcct gtttagcgcc
     9481 tggatgttag ccatccagta ttgtgattac cccttcgata accacaaagt gccgtttaaa
     9541 gagatgcgca ccgccctgcg cacacgcaaa atcaccaata tgcagtttgg tgctttaacc
     9601 agcctgttta cgatgatccc gctgcttaat ctgttcatta tgcccgttgc cgtttgtggc
     9661 gcgacggcga tgtgggttga ttgctatcgc gataaacacg ccatgtggcg gtaacaatct
     9721 accggttatt ttgtaaaccg tttgtgtgaa acaggggtgg cttatgccgc cccttattcc
     9781 atcttgcatg tcattatttc ccttctgcat atagatatgc taaatcctta cttccgcata
     9841 ttctctgagc gggtatgcta cctgttgtat cccaatttca tacagttaag gacaggccat
     9901 gagtaagatt tttgaagata actcgctgac tatcggtcac acgccgctgg ttcgcctgaa
     9961 tcgcatcggt aacggacgca ttctggcgaa ggtggaatct cgtaacccca gcttcagcgt
    10021 taagtgccgt atcggtgcca acatgatttg ggatgccgaa aagcgcggcg tgctgaaacc
    10081 aggcgttgaa ctggttgaac caaccagcgg taataccggg attgcactgg cctatgtagc
    10141 tgccgctcgc ggttacaaac ttaccctgac catgccagaa accatgagta ttgaacgccg
    10201 caagctgctg aaagcgttag gtgcaaacct ggtgctgacc gaaggtgcta aaggcatgaa
    10261 aggcgcaatc caaaaagcag aagaaattgt cgccagcaat ccagagaaat acctgctgct
    10321 gcaacaattc agcaatccgg caaaccctga aattcatgaa aaaaccaccg gtccggagat
    10381 ctgggaagat accgacggtc aggttgatgt atttattgct ggcgttggga ctggcggtac
    10441 gctgactggc gtcagccgct acattaaagg caccaaaggc aagaccgatc ttatctctgt
    10501 cgccgttgag ccaaccgatt ctccagttat cgcccaggcg ctggcaggtg aagagattaa
    10561 acctggtccg cataaaattc agggtattgg cgctggtttt atcccggcta accttgatct
    10621 caagctggtc gacaaagtca ttggcatcac caatgaagaa gccatttcta ccgcacgtcg
    10681 tctgatggaa gaagaaggca ttcttgctgg tatctcttct ggagcagctg ttgctgcggc
    10741 gttgaaacta caagaagatg aaagctttac caataagaat attgtggtta ttctgccgtc
    10801 atcgggtgag cgttatttaa gcactgcatt gtttgccgat ctcttcactg agaaagaatt
    10861 gcaacagtaa tgccagcttg taaaaaatgc gtaaaaaagc acctttttag gtgctttttt
    10921 gtggcctgct tcaaactttc gcccctcctg gcattgattc agcctgtcgg aactggtatt
    10981 taaccagact aattattttg atgcgcgaaa ttaatcgtta caggaaaagc caaagctgaa
    11041 tcgattttat gatttggttc aattcttcct ttagcggcat aatgtttaat gacgtacgaa
    11101 acgtcagcgg tcaacacccg ccagcaatgg actgtattac gctcttcgtg cgtcgcgtct
    11161 gttaaaaact ggcgctaaca atacaggcta aagtcgaacc gccaggctag actttagttc
    11221 cacaacacta aacctataag ttggggaaat acaatgttcc agcaagaagt taccattacc
    11281 gctccgaacg gtctgcacac ccgccctgct gcccagtttg taaaagaagc taagggcttc
    11341 acttctgaaa ttactgtgac ttccaacggc aaaagcgcca gcgcgaaaag cctgtttaaa
    11401 ctgcagactc tgggcctgac tcaaggtacc gttgtgacta tctccgcaga aggcgaagac
    11461 gagcagaaag cggttgaaca tctggttaaa ctgatggcgg aactcgagta atttcccggg
    11521 ttcttttaaa aatcagtcac aagtaaggta gggttatgat ttcaggcatt ttagcatccc
    11581 cgggtatcgc tttcggtaaa gctctgcttc tgaaagaaga cgaaattatc attgaccgga
    11641 aaaaaatttc tgccgaccag gttgatcagg aagttgaacg ttttctgagc ggtcgtgcca
    11701 aggcatcagc ccagctggaa acgatcaaaa cgaaagctgg tgaaacgttc ggtgaagaaa
    11761 aagaagccat ctttgaaggg catattatgc tgctcgaaga tgaggagctg gagcaggaaa
    11821 tcatagccct gattaaagat aagcacatga cagctgacgc agctgctcat gaagttatcg
    11881 aaggtcaggc ttctgccctg gaagagctgg atgatgaata cctgaaagaa cgtgcggctg
    11941 acgtacgtga tatcggtaag cgcctgctgc gcaacatcct gggcctgaag attatcgacc
    12001 tgagcgccat tcaggatgaa gtcattctgg ttgccgctga cctgacgccg tccgaaaccg
    12061 cacagctgaa cctgaagaag gtgctgggtt tcatcaccga cgcgggtggc cgtacttccc
    12121 acacctctat catggcgcgt tctctggaac tgcctgctat cgtgggtacc ggtagcgtca
    12181 cctctcaggt gaaaaatgac gactatctga ttctggatgc cgtaaataat caggtttacg
    12241 tcaatccaac caacgaagtt attgataaaa tgcgcgctgt tcaggagcaa gtggcttctg
    12301 aaaaagcaga gcttgctaaa ctgaaagatc tgccagctat tacgctggac ggtcaccagg
    12361 tagaagtgtg cgctaacatt ggtacggttc gtgacgttga aggtgcagag cgtaacggcg
    12421 ctgaaggcgt tggtctgtat cgtactgagt tcctgttcat ggaccgcgac gcgttgccca
    12481 ctgaagaaga acagtttgct gcttacaaag cagtggctga agcgtgtggc tcgcaggcgg
    12541 ttatcgttcg taccatggac atcggcggcg acaaagagct gccatacatg aacttcccga
    12601 aagaagagaa cccgttcctc ggctggcgcg ctatccgtat cgcgatggat cgtaaagaga
    12661 tcctgcgcga tcagctccgt gctatcctgc gtgcctctgc tttcggtaaa ttgcgcatca
    12721 tgttcccgat gatcatctct gttgaagaag tgcgtgcact gcgcaaagag atcgaaatct
    12781 acaaacagga actgcgcgac gaaggtaaag cgtttgacga gtcaattgaa atcggcgtaa
    12841 tggtggaaac accggctgcc gcaacaattg cacgtcattt agccaaagaa gttgatttct
    12901 ttagtatcgg caccaatgat ttaacgcagt acactctggc agttgaccgt ggtaatgata
    12961 tgatttcaca cctttaccag ccaatgtcac cgtccgtgct gaacttgatc aagcaagtta
    13021 ttgatgcttc tcatgctgaa ggcaaatgga ctggcatgtg tggtgagctt gctggcgatg
    13081 aacgtgctac acttctgttg ctggggatgg gtctggacga attctctatg agcgccattt
    13141 ctatcccgcg cattaagaag attatccgta acacgaactt cgaagatgcg aaggtgttag
    13201 cagagcaggc tcttgctcaa ccgacaacgg acgagttaat gacgctggtt aacaagttca
    13261 ttgaagaaaa aacaatctgc taatccacga gatgcggccc aatttactgc ttaggagaag
    13321 atcatgggtt tgttcgataa actgaaatct ctggtttccg acgacaagaa ggataccgga
    13381 actattgaga tcattgctcc gctctctggc gagatcgtca atatcgaaga cgtgccggat
    13441 gtcgtttttg cggaaaaaat cgttggtgat ggcattgcta tcaaaccaac gggtaacaaa
    13501 atggtcgcgc ctgtagacgg caccattggt aaaatctttg aaaccaacca cgcgttctct
    13561 atcgaatctg atagcggcgt tgagctgttc gtccacttcg gtatcgacac cgttgaactg
    13621 aaaggcgaag gcttcaagcg tattgctgaa gaaggtcagc gcgtgaaagt tggtgatact
    13681 gtcattgaat ttgatctgcc gctgctggaa gagaaagcca agtctaccct gactccggtt
    13741 gttatctcca acatggacga aatcaaagaa ctgatcaaac tgtccggtag cgtaaccgta
    13801 ggtgaaaccc cggttatccg catcaagaag taattcttgc cgcagtgaaa aatggcgccc
    13861 atcggcgcca tttttttatg cttcagccag aggcggcaaa atcaattcat cgctctcatg
    13921 ctgctgggta tagcgcatca cttccagtac gcgcaacccc gcgcggtgca ccgcatcggt
    13981 taacgccttc cctttcagca aaccactgat aagctgagca caaaacaggt cgccagtccc
    14041 tttcaggtca gttttaaccc gggaatggga aatgacattc acgctgtcgg cactgaccac
    14101 cacaacctgc atctcctgat tttcttcatt gccggaggcg ctggtaatca ccacccactt
    14161 taatgtgtct gaaagcagac tttttgcggc agcaatggca ctgtcgagat cgcggcaatc
    14221 tttaccggtc aggatttcca gctcaaagat attgggggta attccctgtg ccagcggcag
    14281 taaatattgt cgatacgcct cgggaaggtc aggtttgaca taaattccgc tatcaatatc
    14341 gccaatcacc ggatcgacca tgatcaatag gtcaggatgg tctttgcgta gcgcagtcag
    14401 ccactcggca aggattttga tttgcgatgc cgttcccata tagcccgtgg ttacagcacg
    14461 aagttggcgc agcgcatcac gctcctgaag cgcacgcaaa taaccgctaa accattcatc
    14521 cggaatcgca ccaccgtaga aagtgtcata atgcggcgta ttgctcagca ataccgtcgg
    14581 cacggcaaag acattcaggc cgttctgctt tatagcaggc accgcaatgc tgttgcctac
    14641 gctgccgtac actacctgcg actgcacggc gacgatatcc gcctgcagtg ccctgctctt
    14701 atcgttaaac aacaacaaac tactcattta attttttctc cttgccgatg atcctcatcg
    14761 tattccagcc gaaactttac ctgattctgg cagtcaaatc ggctattaca aaacaaggat
    14821 aaggtaattc agtgaagaaa atcatttgtc tggtcgttac gctgctaatg acactccccg
    14881 cttatgcgaa gttaacggcc catgaagaag cccgtatcaa cgccatgctg gagggattag
    14941 cacagaaaaa ggatttgata tttgtgcgca acggtgatga acatacctgc gatgaagcgg
    15001 tttctcatct gcgcctgaag ctgggcaata cccgtaatcg cattgataca gccgagcagt
    15061 ttattgacaa ggtggcctcg tcgtcatcga ttaccgggaa gccgtatatc gtgaagatgc
    15121 ccggtaagag cgatgagaac gcacagcctt ttttacatgc gttaattgcg cagacggata
    15181 aaacggtgcc tgcgcaataa tcttaaatcc ccgccccctg gctaaaatgc tcttccccaa
    15241 acaccccggt agaaaggtag cgatcgccac gatcgcagat gatcgccacc accaccgcgc
    15301 cagggttagc ttttgccacc cgcagtgctc cggcaaccgc gccgccggag cttacgccgc
    15361 agaaaatccc ttcccgcact gccagttcgc gcatggtgtt ttccgcatcg cgctgatgaa
    15421 tatccagcac ctcatccacc agagaagcat tgaaaatccc cggcagatat tccgcaggcc
    15481 agcggcgaat gccgggaata ctgctgcctt cttccggctg caggccaaca atggtcaccg
    15541 gtttggattg ttcgcgcata aagcgtgaca cgccggtgat agtgccggtt gttcccatgc
    15601 tggagacaaa atgggtaata cgcccgccag tttgctgcca gatttccggc ccggtagtgg
    15661 tgtaatgggc gtaagggtta tcgggattat tgaactgatc gagcaacttt ccttcgccgc
    15721 gattcgccat ctccagcgcc agatcgcgtg cgccttccat gccctgttct ttggtgacca
    15781 gaatcagttc tgcaccgtag gcacgcatcg ccgcacggcg ttcctggctc atgttgtcgg
    15841 gcatcagcaa tttcatgcga tagcctttaa gcgccgcaat cattgccagc gcaatgccgg
    15901 tgttaccact ggtggcttcg atcagcacat cacccggttt aatctcgccg cgcttttccg
    15961 cctcgacaat catcgaaagc gccgcacggt cttttaccga tcccgccggg ttattgcctt
    16021 ccagttttaa ccacacttca ctgccgttat ccggcgccat tcgctgcaac ttcaccagag
    16081 gcgtattgcc tattgtttgt tctaatgtac tcacgatctc tgtccatacg tggtgttgcc
    16141 tgatgcgacg cttacgcgtc ttatcagacc tacaggttac aaaaccttgc cattaaaaaa
    16201 cccggatcgc gggaagcgcc tccgggcgtt taacattcac tcaacctatc aggcgctttg
    16261 tgcgagagca agttcctcat cgcgggtttc aatacgctca tcgccgttat acagccgcgc
    16321 atgttgcaga ccaacgtata aacgatcgcc acgctgcggg gcatcgtcgc catgcatcac
    16381 gaccgtcagc ggttcgttgt accaccccag cggctgcacc actaattggg tgtagtgacc
    16441 tttcgggctg gcttccagta cctgtacggg tagcggcgaa tcgaggctgg tacggcggct
    16501 gatatccact tcccacgggc gcaggaagag atccaccggc ccctgatacg cgggggtgta
    16561 tcccagcggc cagcgatgtg cgccaacgtg gaactgcccg ccgcgaatgg ttccctgcag
    16621 gcggtttacc tcgcccataa actccagcac aaaacgagtc gccggttcac gccatacctg
    16681 atccggtgcg tcagcttgtt caatattgcc ctgactcatc accactacac gatcagctac
    16741 ttcggtcgcc tcttcctggt cgtgggtcac gaacacgctg gtgaatttca gttcttcatg
    16801 cagttgacgc agccagcgac gcagctcttt acgcacctgc gcatccagcg cgccaaacgg
    16861 ttcatcaagc agcaaaattt gcggttccac agccagcgcg cgtgcaagcg ccacacgctg
    16921 tttctgaccg ccggaaagct gcgccggata acgatccgcc agatgagcaa gctgcaccat
    16981 ctccagcaat tttgtcactt tcgctttgat cgccgccgca ttcgggcgtt cacgccgtgg
    17041 caacaccgtc aggccaaaag cgatattgtc aaacaccgtc atatggcgaa acagcgcgta
    17101 atgctggaac acgaaaccga ctttacggtc ccgcgcgtgc agacggctca cgtcggtacc
    17161 gtgaaagcga atatgcccac tggtttgatg ctccagcccg gcgataatgc gcagcagcgt
    17221 agtttttccg gaaccggacg gcccaagcaa cgcgaccatc tgaccagaag gaatatccag
    17281 tgagatatcg ttcagcacct gggtgcgacc aaaagacttc ttaatattgg caatctcaat
    17341 gctcatgatg ttcctcctgc tgcgcgcgtt tttcctgatt ttccaggcgc cactgcaaca
    17401 tactctttaa aaacagggtg ataatcgcca tcagcgtcaa caacgcggcg gcggtaaagg
    17461 agccgacggt gttgtagtcc tgctccagca attcaatctg taacggcagc gacagggttt
    17521 cgccacgaat cgagccggaa accaccgata ccgcgccaaa ctcgccaatc gcgcgggcgt
    17581 tggtcagcac cacgccgtaa agcagcgccc agcggatgtt cggcagcgtg acgcgacgga
    17641 acatctgcca gccggacgcg ccaagtaaaa tcgccgcttc gtcttcctgg ctgccctgac
    17701 ttaacattac cggtaccagt tcgcgcacca caaacggaca cgtcacgaag atggtgacca
    17761 gcaccatacc cggccaggag aacataattt gcagattatg ttcatccagc cagccgccca
    17821 gcgggccgtt agagccgtag aacagcagat acaccagacc agcaaccacc ggcgacacgg
    17881 caaacggaat gtccagcagc gtcagcaata gctggcgtcc ggggaagtta aagcgcgtca
    17941 ccagccaggc cagcagaatg ccgaacacca ggtttaccgg tacggcaatc agcgcgatca
    18001 tcaccgtcag ccagatggcg tgcagcatgt ccggatcggc cagattttgt aacaccggca
    18061 tcagtccttt gctgaacgcc tgtacgaaga tataaatcat cggcaccagc aggatgaacg
    18121 ccgataccag catcccgatg ccaatcagaa accatttgcc ccagttaatc gggcgcgcgt
    18181 cataacgctt caattgtgtc acttccgcca ttaatgacct accacacgcc gaccaaagcg
    18241 actttgcaga gtgttaattg agaacagcaa cagcagcgat gccgcgagga tcaccgaggc
    18301 aatcgcgctc gccgccgggt aatcaaactc ctgtaagcga acaaagatca tcagcgacgt
    18361 cacttctgtt ttccaggcga tattcccggc gataaaaatc accgcgccaa actcaccgag
    18421 gctgcgggta aacgacagcg ccacacccgc caccagtgcc ggagatagtt ccggcagcac
    18481 cactttgcga aaactctgcc agcgtgttgc gccgagcgtt tctgccgcct cttcatattc
    18541 cgggcctaac tcttccagca ccggctgcac ggtgcgcacc acaaacggaa tgctggtaaa
    18601 ggccatcgcc accgcaatcc ccagccaggt ataggtgact ttgatatcaa acttcgccag
    18661 ccattcaccg taaaaaccgt tcacggaaaa gagcgaggcc agcgttaaac cggcgactgc
    18721 cgttggcagc gcaaagggta aatccatcag cgcatcaagc agcgtgcgac ccggaaagcg
    18781 atagcgggtt aagatccacg ccatcagcag accgaaaacg ccgttaaaaa tcgatgccac
    18841 aaacgccgac agcagcgtca ctttataggc tgcgaccacc tgcgggttgg tgatcacctc
    18901 ccagtactgc gcccagctca tctgggccag ttgcatcacc agcgcggaga gcggcagcag
    18961 caaaatcagg cacacaaaca gcagactggt gccgaggctt aaggtaaagc ccggcagcac
    19021 gcgtcttgaa gaaactgcaa acattactta cgccccgccg ctaacagctt gtctaactcg
    19081 ccgccgctgg tgaagtgggt tttcatcact tccggccagg agccaaattt atcttccacg
    19141 cggaacagct cggtctgcgg gaatttatct ttcagtttgc ccatgacttc cgggttattt
    19201 acgcggtagt aatagtcggt gatgatggtt tgcgcctgcg ggctgtagag ccagttcagg
    19261 taggcttttg ccgctttttc cgtgccgttg gcctgtacgt ttttatccac ccacgccacc
    19321 gggaattccg ccagaatgtt ggttttcgga atcaccactt caaagccctg cgcttcatac
    19381 tgtttacgga tgttgttcac ttccgactcg aagctgatca gcacatcgcc caggccgcgc
    19441 tcggcaaaag tggtggtcgc gccacgaccg ccagtatcga acacttcaac gtttttcagg
    19501 aactgggtca taaactgttc ggttttggct ttatcgccac cgtcggcttt atccgctgca
    19561 ccccacgcgg ctaaataggt gtaacgcgcg ttaccggagg ttttcgggtt cggaaaaatc
    19621 agcttcacgt cggagcgcac cagatcgttc cagtcgtgga tattcttcgg gttaccctta
    19681 cgcaccagga agcccatggt ggagtagaac ggcgagctgt tgtttggcag acgcgactgc
    19741 cagtcagccg gaataagctt gcctttgtcg tgcaggatct gcacgtctgt cacctggtta
    19801 taagtgacaa cgtcggcttt taagccctgc aaaatcgcca gcgcctgttt tgatgacccg
    19861 gcatgagatt gttttatcgt cagtttgtcg ccgccgttat ctttggccca ttgctgttca
    19921 aacggcgggt tcagggcggc aaacagctcg cgcgagacgt cataagaact gttcagcagt
    19981 tccgttgcct gtacatggcc cgccagcagc agagaagcga ccagcacgag tgagttcttt
    20041 ttcagtaagt taacggccat tgcgcaccct tataaattta atgactttct aatagccatc
    20101 atatttataa cggacgtgaa aggagtaacg gttttatata ccgtttggtg atttggaagt
    20161 tgaaaaggga ataagacgaa atcctcccca caaatgcagg gaggaaacag aggtgaatca
    20221 gatgccgacg ctaaccgtct ccggcagtgt gctgccgcca tcaatcacat tctgtgtacc
    20281 tgttaaatag ctggattcat ccgatgcgag gaaggccgcc agttcgccga cttccagcgg
    20341 atcggcgagg cgacgcatcg ggattgcttt cgccatttca gtcagcaccg attctggatc
    20401 ttccgggttc gactggcggg caatgctttc cgccattggc gtgcgaacgt atccagggca
    20461 gatggcgtta acgcgaatac cagactgcgc gtactccacc gccagcgatt tagtcaggcc
    20521 aacaatcgcc gctttcgtta aggcgtacgc cgtttcgccc gggtctgcca ccatatcgcc
    20581 agtgactgaa gacatcatca caatgcggcc atctttgcgg gcaatcatct ccggcagcac
    20641 cgccttcgtg acgttccata cgccttttat attgatatca atatggaaat cgcgatcttc
    20701 atcgctcata tcgaggaagc tgcccagacg acaaacgcct gcgttattca ccagaatatc
    20761 aatgcgtcct tctttttcct tagcgcgctt aatagctgcg gctaccgacg ccgggtcacg
    20821 cacatcggcg acaaccgccg tacagcgatg accacgacca cacagttcgt ccgccagctt
    20881 ttcgatctca ggggagatat ccagcaagat taggtttgcg ccatgacgtg caaaagttct
    20941 ggcaattcct tcgccaattc cctgcaatgc gcccgtaatc agtgctgtct tgcccgtgag
    21001 tttacccatt ttaatgctct ccttgttaat acagagcgtt acactccatt ccctctgaaa
    21061 atacaggata aacaaaatat tgctgcggat cagctcacta attcaggaaa atctcaattt
    21121 agtcgctgct ttatgcagca cctcaagcgt tacgtcagcg cgcaatcatt ttcagtgaca
    21181 tcacatgagt aatattcgga cttaatgatt cctttgtgtc tatcaaatcg ggatatatga
    21241 tcccatttat ctgcaattat ttgatcgcaa atattaaggt caccaccgat gcaacttgaa
    21301 aagatgatta ccgaaggctc gaacgccgcc tcggctgaaa ttgaccgcgt atcaacgctg
    21361 gaaatgtgcc ggattatcaa cgatgaagat aaaaccgtac cactcgccgt tgagcgcgta
    21421 ctgccggata tcgccgcggc gatcgatgtt atccacaccc aggtaagcgg cggcggccgt
    21481 ctgatttatc tcggtgcggg aacatccggt cgtctgggga ttctggatgc cagcgaatgt
    21541 ccgccgacct acggcgtgaa accgggcctg gtagttggtt tgattgctgg cggcgaatat
    21601 gccattcagc acgcggtgga aggcgcagaa gatagccggg aaggtggtgt taatgatctg
    21661 aaaaatatcg gcttaaccgc gcaggatgtg gtggttggta ttgcggccag cggacgcacg
    21721 ccgtatgtga ttgccgggct ggaatacgcg cgccaactcg gctgccgtac agtgggcatt
    21781 tcctgtaatc cggggagtgc cgtttcatcc accgcagagt ttgctattac gccggtagtt
    21841 ggtgccgaag ttgttaccgg ttcttcgcga atgaaagcag gcacggcgca gaaactggtg
    21901 ctcaacatgc tctctaccgg gctgatgatt aaatccggca aagtgttcgg caacctgatg
    21961 gtcgatgtgg tcgccaccaa cgaaaaactg catgtgcgcc aggtcaatat cgtcaaaaac
    22021 gctaccggat gtaatgccga acaagcggaa gcggcgttaa ttgcttgcga gcgcaactgt
    22081 aaaacggcca ttgtgatggt actgaaaaat ctcgatgccg ctgaagctaa aaaatgcctg
    22141 gatcaacacg gcggttttat tcgcaaggct ctggaaaagg aataacccat ggctaaagag
    22201 atcagcagtg aacttctaaa caccattctt acccgggtcg gcggaccggg aaatatcgcc
    22261 agttgtggta actgtatgac gcgcctgcgt ctgggtgtac atgacagttc actggttgat
    22321 cccaatatca aaacgctgga aggcgtgaag ggggtcattt tgaccagcga tcaggtgcag
    22381 gtggtttttg ggccaggcaa agcgcatcgt gccgcaaaag ccatgagcga gctgctgggc
    22441 gaggcaccgg tacaggatgc cgcagaaatc gccgcccaga ataaacgcca gttaaaagcc
    22501 agacaaactt ccggcgtgca acaatttctc gccaaattcg ccaccatctt cacgccgctg
    22561 atccccggtt ttattgccgc cggtctgctg ctggggatag cgacgttaat tgccacggtg
    22621 atgcacgttc cggcagatgc tcagggcacg ctacccgatg cgctgaattt tatgaaggtg
    22681 ttcagcaaag gcctgttcac cttcctggta attctggtgg gctataacgc ggcccaggca
    22741 tttggtggca cgggcgtaaa tggcgcaatt atcgctgcgc tctttttgct cggttataac
    22801 cccgctgcaa ccacgggtta ctacgccgga tttcacgatt tctttggtct gcctatcgat
    22861 ccgcgcggca atattatcgg cgtgttgatt gccgcatggg cctgcgcccg catcgaatgc
    22921 atggtgcgcc gctttatgcc ggacgacctc gacatgctgt tgacctcatt aatcaccctg
    22981 ctgatcaccg ctacgcttgc gtacctgatc attatgccgc tgggcggctg gctgtttgaa
    23041 ggtatgtcat ggctgtttat gcacctgaac agtaatccgc tcggttgtgc ggttttagcc
    23101 gggctgttcc tgatcgccgt ggtatttggc gtgcatcagg gctttattcc tgtttatctg
    23161 gcgttaatgg acagccaggg attcaacagc ttatttccca tcctttcaat ggcaggcgca
    23221 ggccaggtgg gcgcggcgct ggcgctctac tggcgggcgc aaccgcacag tgcattacgc
    23281 agtcaggtcc gcggggcgat tattcccggt ctgcttggcg ttggcgaacc gctgatttac
    23341 ggtgtcaccc tgccccgcat gaaaccgttt gttaccgcct gtttaggcgg cgcggcgggc
    23401 ggtttgttta tcggcctgat agcctggtgg ggtctgccga tgggcttaaa cagcgccttc
    23461 gggccgtctg gtctggtggc gctgccgctg atgacttccg cacaaggcat ccttccggca
    23521 atggcggttt acgctggcgg tattctggtg gcatgggtta gtgggtttat tttcaccaca
    23581 ctctttggct gccgtaacgt caatctggac tgattatgaa acggacaatg ctctatcttt
    23641 ctctgctggc tattagctgt agcgtcagcg ccgcaaaata ccctgttttg gcagaaagta
    23701 gttgatcaag tgtatgcggc gtcaaagcag taataaaaag gccagtcagt tgtgttactg
    23761 gctggccttt aatcaatgaa tcagaaacgc ttacagcgcc atcagcttgt ccagcgacgg
    23821 tgcgaagtaa tagccgccag tgaccggttt ggtgaaacgc aacatcgcat cacgcttacc
    23881 atcggtatcg ccaaacatgc tcagcagttg ctgctcaatg ttatgcagac gcgcgcagta
    23941 ggcgcagaag tacagaccgt gagtgccact ggcggtgccg tacggcaggc tctgacgaac
    24001 aatcttcagc cctttgccat cttctttcag atcaacgcgg gtcaggtgag aggtttccgg
    24061 acgttcgtcg ccgtcaatct cttcgttggc ctctttggtg cgcccaatca tcatctcttg
    24121 atcgtgaacg ctcatccgat tgagctgctt caggttgtgc tcccaacgct gcacaaacac
    24181 atagctgccg cccgcatcca cgccgtcttt gataacagcc acttcgcggc gcgtctcttc
    24241 acccgccggg ttttctgtac cgtcaacaaa gccgctcagg tcacgctctt caacccaacg
    24301 gaagccatgg atctcttctt tcacttcaat gcagtcacca aaggcttcca tcgccgcctg
    24361 agcgacagag aagttcacgt cgtgacgcag agaaagaatg tggatcaaca catcgaactg
    24421 ggtcgtcggt gcaagacctt taccgtagcc cggaaaatct ttcagctctt cggccccaac
    24481 gccgccgctc agagcgcgcc aggtattgtt accaaaggca accaccgcac caagatgcgc
    24541 gtccgggaat ttcgcttcaa aagttgccag tttgtcggca aatgttttac tggccgcacg
    24601 cagggcgtca acttcccctt tcacgttggc ttcgatccaa atcgccgcgc ggcaatgttc
    24661 tggcaaaatg ccactctgaa cctgagacat tgttcctcct gaaaataata atgccacgca
    24721 atccgtggca ttgttgggcg ctattttacg tgttttttcg cggcggcggt ttgttcagac
    24781 gcaaattaac gccgccagat aattttactg actttccagt ttttcagggt atcgtccgaa
    24841 ggcattaacc cttcaggacc actccattcc ccgctgaact ggtagctaat atgttgactg
    24901 ccttcggctt tacattcgac ggcacgatta tcatcactgt cggccttttg gcaattgcca
    24961 aatgctttgc tgtaaaggtc actaaacggt gtaccgattt taacaccggt gtcagcagga
    25021 atatcgctat ccagcacatc aatgcggctg atcgtgcctt gatcgccgtt aatcaccatc
    25081 gccacgttgt tgcctttcat cacttcaaaa aagcgcacca cgttgccgtt cgcggttttc
    25141 attccgctgc gcaggcgata atcgccatca agcgcatcag caatggcttg ttcttgcagt
    25201 ggtgtggacg ccgttaattc acccacgccc tgctcgctca ctttggtgga tgatccaaac
    25261 cagttccacg ggttagcggc cgaccagtta actgacgaca tcgtggaaca gccggtcagc
    25321 atcaacggca tagcgcataa cattaaacgc agcgatttca tgtcacttcc tttggttatt
    25381 caataacgtt gcttggagtg caaattcacc aaaaagtgcc gttattcttt ttcttcatta
    25441 aaacaagcgc gtaaacgtcg attggtcagc agccagatta acgccacgat atccgccact
    25501 accagcgcca gaccgatacc gctaacggat tcaccgttca gccacagcca cggttgccaa
    25561 caaagtaaaa ccacttgcgc caacagcaac agaaaataga gcacatgcca gatacgggga
    25621 aatgaagccc gccgaccact cagcaaaaat gccagcaccg ccggaatacc gggaatcaac
    25681 cccagccaga aattatcgtg atcgggataa aacagattta gcagcgcagt accctgctcg
    25741 cgcgacgctc cggcaatgac aaacagcacc caggttcgcg cctgaagcaa tagcacaagc
    25801 cagaagagca agggtaaacg caggcgaccg tgcgcatcat aatggacagg atgaaactca
    25861 gtactcttca tcttcaatca aacgcttacc cagactcagc acgtcggcgt gttcatatcc
    25921 caggcgttca tacattccga gcaccatgtc gttatcttcc ggcacgttga tctgaatttt
    25981 cggacagccg cgagcaatca gctttttctc cagccgatta agcaacgcat tagcaatccc
    26041 acgcccacga aactcgggat gcacgccaag ataataagca gacccgcgat gcccgtcata
    26101 accgcccatc accgttccga ccacttcacc gttcacctca gcgaccagaa acaaactgac
    26161 gtcatggttc atcttacgct cgatgtccat ttccggatcg ttccacggac tcagcaagtc
    26221 gcaacgctcc caaagggtga tgacctcttc gaaatcttcc tggcgaaata cgcgtatctc
    26281 catggtattc gttacctttt tgcgggttaa aaggctgatt atggcgtgaa cggtcgaatt
    26341 agccaatatc tgacgaaaat cggttgaaaa agtggcataa tggggagttg tcaactattg
    26401 aaatgaaaag taaaacaatt ctcaacagca aaccgtcgta acggattacg cgatacgata
    26461 taacatctgg aactttatta ttacaactca ggccgtatga gcacttttaa accactaaaa
    26521 acactcactt cgcgccgcca ggtgctgaaa gccggtttgg ctgccctgac gttgtcagga
    26581 atgtcgcaag ccatcgccaa agaagaaccg ttaaaaacca gtaacggaca cagcaagccg
    26641 aaagcaaaaa aaagcggcgg cagacgtgtc gttgttctcg atccaggcca cggcgggatt
    26701 gataccggag cgatcggacg caacggttcg aaagaaaaac atgtggtgct ggcaattgcc
    26761 aagaatgtcc gttccatatt gcgtaatcat ggcattgatg cgcgtttaac gcgttctggc
    26821 gatacgttta tcccactgta cgatcgcgtt gaaatcgccc ataaacatgg cgcagatctg
    26881 tttatgtcaa ttcatgccga cggctttacc aacccgaaag ctgccggtgc ttcggtattt
    26941 gccctctcta accgcggggc aagtagcgca atggcaaaat acctgtctga acgcgaaaac
    27001 cgcgccgatg aagttgccgg taaaaaagcg actgacaagg atcacctatt gcagcaagtg
    27061 ctgtttgatc tggtgcaaac cgatactatt aaaaacagcc tgacgctcgg ctcgcatatt
    27121 ctgaaaaaga ttaagccggt gcataaactg cacagccgca acaccgaaca agccgcgttt
    27181 gtggtgttga aatcgccgtc ggttccgtcg gtgctggtgg aaacctcgtt tatcaccaac
    27241 ccggaagaag agcggctgtt aggcacggcg gcgtttcgcc agaaaatcgc cacagcgatt
    27301 gccgaaggcg tgatcagtta tttccactgg tttgacaacc agaaagcaca ctcgagaaag
    27361 cggtaagtga tgaaacccga cgcacacctg gttaaacagt tcttgctcaa ccttcaggat
    27421 acaatttgtc aacaactgac cgccgtcgat ggcgcagagt ttgtcgaaga tagctggcaa
    27481 cgtgaagctg gcggcggtgg gcgtagtcgg gtgttgcgta atggtggtgt tttcgaacaa
    27541 gcgggcgtca acttttcaca tgttcacggt gaggcgatgc ctgcttccgc caccgctcat
    27601 cgtccggaac ttgccgggcg cagtttcgag gcgatgggcg tttcactggt agtgcatccg
    27661 cataatccgt atgttcccac cagccacgcg aatgtgcgct tttttattgc cgaaaaaccg
    27721 ggtgcagagc ccgtctggtg gtttggcggc ggcttcgatt taaccccttt ctatggtttt
    27781 gaagaagacg ccattcactg gcaccgcacc gcccgtgacc tgtgcctgcc atttggcgaa
    27841 gacgtttatc cgcgttacaa aaaatggtgc gacgaatact tctacctcaa acatcgcaac
    27901 gaacagcgcg gcattggcgg gctgttcttt gatgatctga acacgccaga tttcgaccac
    27961 tgttttgcct ttatgcaggc ggtaggcaaa ggctacaccg acgcttattt accaattgta
    28021 gagcgacgga aagcgatggc ctacggcgag cgcgagcgca attttcagct ctaccgtcgc
    28081 ggtcgttatg tcgagttcaa tctggtctgg gatcgcggca cgctgtttgg cctgcaaact
    28141 ggcgggcgca ccgagtcgat cctgatgtca atgccgccac tggtacgctg ggagtatgat
    28201 tatcagccaa aagacggcag cccggaagcg gctttaagtg agtttattaa ggtcagagat
    28261 tgggtgtaac tccctcaccc ccactctcgc atccgctgat gcagcgtcag tgacggcttc
    28321 tcggcaaaca gttgctgata atccgtggca aattgcccaa gatgccagaa tccccactgc
    28381 atggcggcgt cttttaccgt tgtgctttgt gaccacggac ttatgagttc gcggcgcacg
    28441 gcgttcaggc gaatacgttt cagccacgcg ttcggaccaa tgcccaaaat ggcgtgaaat
    28501 gcgttttgta gcgtgcggcg gctgacatgc agttgattac acaagtccag caccgtcacc
    28561 ggttcggaca tgttttccag cacatattca cgggcgcggg aaagcaatcg gcggtaactc
    28621 tgatggctga tgctttccgc cgtcatcatc ggctgcgctt cttccagcat cgcccccatc
    28681 gccatgagca aattatcccc cagcactttt cgcactgcag gctgatggag attttccggg
    28741 ttctcgcaaa acgtcgccag cgcctgttgg acaaagcccc acagagcggc tttatgctgc
    28801 tctttcactt ccagcgccga ctggctacgc agcatatgta atacccgatc cggattatgc
    28861 aaaaagttag cctgacgagt gatgacatct tcagaaagca ctacgccaag gatcgtgtaa
    28921 tcgtccggcg tacttaattc aaactcggtt ccacccgggc gcgtcgcaat ttctgcgctt
    28981 cccagacatt gcgaaccgat aaatcccagc tcaccgcgcg tcgccggaat gccaaaccag
    29041 aacgagttcg gccagaccag gcacgactga cgcagcgcca gaccagtgta ttcacgaaaa
    29101 acctgaatat catcgagtag aatttccgta aattcaccat gaaacttgcc cggatgcagc
    29161 tgatcgtaaa tctgctgcca ggcggtaatt gtcaaagcat gttcatagac gtccgttgtc
    29221 tgtcgttgat gaacattatc cacttcgacc ttcggcgtga gcttcaggtt ttcgggtaag
    29281 ggttcatgat aaagatggtg caaattggct gtacgggtct ttttcatgat gttaatgccg
    29341 ggtgttgtag gacacccggc acctccgaca ggttaatggg gcttgagacg gtaacgacta
    29401 ctgcgtttac gtaacgtccc ggcagaaaag agctgttcga gcgcatttct ggctttttcc
    29461 agcggccagc caaagtgggc agccacttct cctgccgtca ttccctgacg tactgatgtt
    29521 aacagcgcca acaactcgtc agcagattcc gcgacgataa ctggcgcgtc gggttccttt
    29581 acgggttgct taggctggcg gttaaagcca gtaaccagcc actgggtgtc atcgtctggc
    29641 cgaccgatct ccttgcggct gatgacacgc ccggtacgca tcgcggcagc acaacctgcg
    29701 tccagcgccg cacgacacgc cgccagatcg ccttccacca ccagcgttag gcgaccaggg
    29761 tcaagcactt cgtgactgag cagacgaacg ttagctgctt tgagcatggc atcagctgca
    29821 tctatcgcgg cgaccatgcc gtccacttcc agcaatccca gtgcattgat catcggcaac
    29881 ctccgttacg cacgctggat tggattacgc gcgatttcca gcactgcatc ggtaaaggcg
    29941 ttacaggctg ctttacacgc ggcctggcta ccggttaaaa atgctgccga gtagttggtt
    30001 tcagacggtg gcgggacata ggtcaccagc tgcacgtcgg cagatttcaa cgctgcatca
    30061 atgccgtagg tcgcttccag cggcggtgcc accagatacg ccatcggatc gccaagcgtg
    30121 atcccggcgg ttgatgagag ataagaaccg gtacgcgaaa ctacatgtgc caggaaggct
    30181 gtgttttctg cgtcattagc ccactggaaa gcagcgccat tttcaatatt cgcaaccatc
    30241 gcatccagac cagcacgcac ttccgccggg tttggtccac caagcataat cagcacctca
    30301 ccggcagtcg gtgacgggcc gtgtgcagcg cccgcataca gcgagcgacc atataccact
    30361 tcgaccatcg cctgcttggt cgcttcgtca gccgcaatgt aggtgacgtc atctgaatca
    30421 gcagaaatca gcccgaggct acgaatatgc ggcggcaatt tcagttcacg tgcaaattcg
    30481 gcgttaacgg aggcaatcac ccgcatggcg gttaccgacg gtcgaatcaa atctaaagct
    30541 ggcatgatgc ctccttatcg ggtcatgttg atgccggatg ctttctgctc cagcatacgt
    30601 ttcgccaaat ccacaatgac ggctgcggct tcaaccggcg gcgtgccgcc ctggtgaatg
    30661 ttagagatac aagtgcggtc agcctcaacg gtggtcgcca tacgcggcga gtaaacggcg
    30721 tagcaggaga ggctttctga ctgccccaac cccggacgtt cgcccaccag caggatcact
    30781 actttcgcgc cgaggatctc accgatctga tcttcaatct tcacgcgacc ataacgtaca
    30841 aagaatggcg taccgacttt cagcccggct tgcttcaggc ccgccatcag cggcggcagg
    30901 atctcttcgt agttcacggt gatcgcatca gttgacaggc catcagaaat aacgacctgc
    30961 acgtccggat tagcaacaca ctgtgctttc agcgcttcaa cagcttctgc acacaggcgg
    31021 cggcccatat ccgggcgagt caggtagaga tttttgtcgc tgatttcgga gcggacttcc
    31081 agcaagccct gcgctttcac ccactcttcc ggtacttctt tcagaacggt atctttcgaa
    31141 cgagagtgat cggccaggaa acgcagcaac gcctgggtgc gcggacgcgg accggcacga
    31201 ccggtacaaa cgcgggccac ggtgctgcga cgcagttctg ttaatacgtc tgcgtgatgc
    31261 gggttttcaa caccaatcca cgcttttgct tccgcagaac ctaaatccag cgcgcagctt
    31321 tccgaggtca ccggtgccgc acaggtggtg gtggcgcact ttgcttctga cggcgcgggg
    31381 gccgcttgtc ccattgacgc catcacgctg cgtacaattt cttcaatctg tttttgatcc
    31441 atgatgtgtt atctccgcgt catcagaaga acagtgacgg atcgcccgcc cgtttggtca
    31501 ggcgaccgtt tgccataatg cccatgcttt ccagccagcg ttcaaactcc ggtgacgggc
    31561 gcagattcag taactgacgc acagtggcag tgtcgtggaa tgcggtggtc tgatagttga
    31621 gcatgatgtc atcgcccagc ggcatcccca tgatgtagtt gcagcctgcg gtggcgagca
    31681 ggatcatcag gttttcgttg aggttctggt cagcgtcagc gtggttggtg tagcagcagt
    31741 cacagcccat agagatgccg ctcagtttgc ccataaagtg atcttctaag cctgcgcgga
    31801 taatctggcg gtcgttgtag agatactccg gcccaataaa gccgaccaca gtgttgacga
    31861 taaacggatc gtaatgacgc gccagcccat agttacgcgc ttccatcgtc acctggtctg
    31921 cgccgaagtt cgcgccagca gacagcgccg aaccttgtcc ggtttcgaag tagaggcagt
    31981 tttccccggc gatacggttg aactccgcgc ccactgcgcg tgcttcatcg agcatcgcca
    32041 gctctacgcc aaactctttc agcccttttt cgctgccgca gatactctgg aaaatcagcc
    32101 cgcctggtgc gccgcgacga atcgcttcga tttgggtagt gacgtgcgcc agtacgcagc
    32161 cctgagttgg gatgttgaat ttgtcgatca cgccatagat ggtatccaac acgcggctta
    32221 agttttccac gtcgtcagtc accgggttaa cgccgatcac cgcatcgccc accccgaagg
    32281 aaagcccttc gtagatttgc gcggcgatac tttgcacgtc gtcacgggta tcgttcggct
    32341 gcaagcgggc gctgaaggtg cccggaatac cgatggtggt attggccttt ttgatcaccg
    32401 gcattttctt cgcgccgtag atcaggtccg cgttggagca aatcttcgct accgccgcca
    32461 ccacttccga ggtcagccct ttgcgggtaa aggcaatgtc atccacgctg gtttcatcgc
    32521 tcagcacata ctcacgcagt tcgctgatgc tccagttttt aatctggttg taggccgttt
    32581 cgttaacgtc gtcctgaatc agccgcgtca cgcagtcatc ttcataggca atcaccggat
    32641 tattgcggat gtccgctaca gtcatttccg acaacacctg ctttgccgcc acgcgctcct
    32701 gtgagcttgc cgcagcaacg cccgccagca catcccccga acgcagttcg ttggctttag
    32761 ccagcacctc ttttacatcc ttaaactgat atacattgcc gaacaatgtg gtctttagtt
    32821 tcataagtcg ttccctcagg aaggaaatgc gagtgatttc accgtcaccg gcacaaccga
    32881 tccgccaaaa agaggcgtac caatgtcgat atagtccccc gcgcggacaa tcacttcgtc
    32941 aatgactgcc aacgggagtt gttgtagctg tgggcgcaac aacatgccca gagctttacc
    33001 aaagtcctgc ccggccacca ccagcagggg atgcggattc ggaaaacgcg cgacgaaatc
    33061 gaccagcgcg ttgatgaccg tcagtaccgc ggcgtaacgc acaggcagcg aggcgggaag
    33121 cgccagcacg tacgcgtcag ttttggggca aagatccagt tgaatcagcg cctgttgcca
    33181 ggcactgacc agatccgttt catcaatcgg gatcgccacc ggcaaattgc gcagcggcag
    33241 ttgtacgccc tccagccata ttgtgctgcc agagagcgaa agggtatgtg cacccgcgcc
    33301 aatcaccgtg gcgcgcacgg tttgcgccgg aaactgcacg ttcatctcac gcagacgcgg
    33361 atggtcatgc agcgccgttg ccagaagcgg gccgatatcg gcaaaacaga acgggtcggc
    33421 gggctggtgg cgataacatt cgcctacccc gccagaaagc gtaatgattt cgggcgtaac
    33481 acctgcgggc agcaaaccgg tttgcatcaa tgcctgcgcg agcggcgaga gcgttccgtc
    33541 aatcacttcg acaatcagtt ccgccatccg gcgggtaacc tgcgccaatt gcgcgccggt
    33601 cagcgaacgg gcgtcagtgc ctgggccgaa gcactcatcc acaatcatct gccccggttt
    33661 atgagcgtaa accacgcgcc cctggctgtc ggtttccagc aggcgaccgc cgacgttgag
    33721 gcaggcagtg ccgctgattt ttccggcatc gaacagggcg tagttcgcgg tgccaccgcc
    33781 gatgtcgata ttcagtaccc gacacagccg ttgttcagaa agggtttgcg ccccggcacc
    33841 gtgaccggcg atcacggatt cgagatgcgg cccggcgctg gcgacgacaa aatcgcccag
    33901 cgaccgagag agcgccatca ccgccgggcg agcgttgcgg gttttcgcgc tttcaccggt
    33961 gatgatgatg gcaccagaat caacgctttc cggcgcaatg cccgcagcct gatattgctc
    34021 gaggattaag gttttcagtt ccgcttcttt taaaccaccc tgtttatcga caggggtaaa
    34081 gaacaccggg ctttgccagc taatttcgcg tttgatgaat tcgtagcgcg gcacctgcga
    34141 caccgccgca cggttaacca gctccaggtg ggagaagatc acctgggtgg tggtggtgcc
    34201 gatatcgata ccgacgctca gtagctggcg agtgttcacg attgcgcctc cgcttcggtt
    34261 ttagccgcgc tcgcgtcttc ttttggcacc agcatcatcg ccacgccaat tgccgttacg
    34321 ccgccgatca atttgccgac aatcatcggg aagatcatgg cgttcatgtt ggcagcggcg
    34381 aagcctaaat ggtcgcccag cgcgaaagca gcggaaacgg cgaaggcaca gttgatgact
    34441 ttgccgcggg tatccatctg cttcatcatg ccaaacatcg gaatgttgtt ggcaagcgtt
    34501 gccaccatgc cggctgccgc gatattgttc atattcagca ccttaccgac gctcatcagc
    34561 ggtttttcaa accagcgagt cagcagcagt accattggat acgcccctaa cagaacgcag
    34621 gagatcgagc cgataacttc aatggcgcgc atcacttcac cgggtttatc gccaggggcc
    34681 ataaagatag gatcaaggcc cgggatcagt tcccagccaa ggaggaattt cactaccgca
    34741 gcggcaagac cgagggtgat caatgcaacg aggaatttgg cgaagatctg gaagccgttg
    34801 atcatttttt ccgggatgaa tttcagcccc agcgccacca gcaccgcaac gataagtacc
    34861 gggatcatgt tcatcaggat cagcgcaaag gtgaattcaa caggctggcc gttgatctgc
    34921 acaccggagt acatcgctac cagaccacca gcgatacagc caatcggaat ggtcactatg
    34981 cccgccagca cgccgagcgc cagataacga cggtcagaag gttcgataat gccgagcgcc
    35041 accggaatgg aaaacacaat cgttggcccc atcatcgacc cgagaattaa cccagagtat
    35101 agccacgctg ctacgtcacc gcccgccagc tctttggcga ggaagaagcc gcccatatcg
    35161 cacgccagca gtgttccggc gaacattgac gggtttgcgc cgagcatttc gtaaaccggg
    35221 ataatcaccg gtccgagaac gtgggccagt accggtgcca gtgcggtcat accgaccata
    35281 gccaggccca gagcgcccat tgccataaag ccttcttcga actgaccgcc ggatccttcg
    35341 atacttttac cgaacttacc gaggaaacga gcagaaccgc cgaactgcga caggatcctg
    35401 tctacggcag ctatcagcat aaagaacatc atgatgtaca tgatgatttc gttaattccc
    35461 atcgccttta ctccctgtta gttgttattt attggcggat gcggcgtgaa cgccttatcc
    35521 gccctacatg tgcaatcccg aaggccagat aagacgcggc aagcgtcgca tccggcaatt
    35581 gcaccgcgcc actggcggat gcggcgtgaa cgccttatcc gccctacatg tgcaatcccg
    35641 taggccggat aagacgcggc aagcgtcgca tccggcattt attgcgccgc tgcgtacagg
    35701 ccgacaatct gctcgaggct ggcggtacgc gggttactgc gcagacaaat atcttcctgc
    35761 gcggcctgcg cccatgcacc gtaatgcccg gttgttgcac ccacatcagc cagccgcttg
    35821 ttgatcccaa cttccgctat cagctcactt accgcattaa tagcgtcacg atcgtcggat
    35881 tttttagttc gcagcgcccg accaatctga ctaaaccgat cacgacaaac catccggtta
    35941 aattccatca ccgttggcag caacatggcg ttcgccagac cgtgcggaat atgcagcgcc
    36001 gcccctggct gatgcgccat cgcgtggcat aacccaagcc cggcgctgga aaacgccatt
    36061 cccgccatac atgaagccag caacatgctc tcgcgggcga caaggtcgtg accgtagccc
    36121 accgctttcg gcagcgattg accaatcatc gcaatggcgc caatcgccag gctgtcggta
    36181 aacggtgtag cgttcagcgc gctgtacgct tcaatggcat gggttaacgc atcaatgcca
    36241 gtcatcgccg tgacatgcga cggcacgcct tcggttagca cagcgtcgag aatcgcgaca
    36301 tccggcatca gcgaggcatg ggctaacacc tgtttgcgcc cgcttaccgc atcgataatc
    36361 accgtcacgt tggtggtttc agagccggtt ccggcggtcg ttggaatggc aatcaacggc
    36421 aggcgcggtt gcagaacgct ggtttctgac atttctgcca gcgtgctatc cgggttcgtc
    36481 accagcaacg ccacggcttt cgccgcatcc agcaccgagc cgccgccaaa cgcgatcacc
    36541 ccgtcacaac ctgattcacg caactgcgcc acggctgcac acacgtcggt gatgcacggt
    36601 tcgcccaccg gacatggcca gagcgtcatg gcgatacctt taacggccag gctacgcgtc
    36661 agcccagcgg tcatccctgc ctgatgcaaa aagctgtctg ccatcacgaa cagatgtttc
    36721 aacccacgcg tttgcgcttg ctgcccacaa ctgctcaccg cgcccggacc gcaaagcgtc
    36781 accggtggaa cgctaaatgt ttttacccgt tgcagattca gggtatcgaa cgcctgaaag
    36841 agcgcggtct gcaattcatt ttgcatatag cccctccgct ttctctcgcc cactactggc
    36901 gattgccagc ggggtcataa acaaactgtg ctgcggtaaa tgcacctgta acgccggaaa
    36961 ttgtttgcaa aacagcgccg ctacgcccgg ttgcatacag gaaccgcccg ccagccataa
    37021 atccgtaatg ccctgccctt caatatgccg ggccacaatg tccgccattt tttcgtacac
    37081 cggtttcacc gcgggccaaa tctcgtcacc gtgaccgcgt ttgtactgct ctgcctcttc
    37141 cagcgaaata cggcgatttc cggcgagagt cagagagatg tgatgtccgc cggtggcttc
    37201 atccgccgag tacgtcactt tgcccttttt cacgatggca atgccggtag tgccgccgcc
    37261 gatatccact acaccggcgt tgtccagttg cagcagatcc gcaactgcgg ttggctcatc
    37321 cagcacatgg ctcacttcca gtccggcaga ctccagcacg ttgatggaaa tacgcgggtc
    37381 cgtacctgga ggaaaagagg tcgccgcatg gctaaaacgg cgaccgaatt gctgctcgag
    37441 ggtgtcgaga tggcgacgaa caatggtgac cgcaccgaag aaatcccaga cgatgccgtc
    37501 gcggaccaca tcagcccagt cgaggcatac cgccaccggc tgaccgtcgc ggtcgacaac
    37561 catcgacacc acatcgcagg tgcccagatc cacccccagc cacagcggag attccgtcgc
    37621 ggcgggcgtc tggttacaca gcgttgccgc cgtttgcaga cgtggggtga gccattgttc
    37681 atcgtgcgcc atcggttact ccttaaacaa tgcgaaacgc atcgactaat acacagcgac
    37741 gcagacggac aaacgtccgc gcgctggtta ccccttcacc ggttggcgtg gtgatggtca
    37801 tcgtggtcca gccttccccg cccagcccca gcccggcaat acacggtccg ttcttaacga
    37861 aaatgctggt atcaatggcg ttcgccatct ggttcatgtt ttcgatattg cgcgagtgca
    37921 ttgccgccgt gtggtggcaa ccgccttcca gtttcaccgc cagcgcaatg gcatccgcca
    37981 cgttggcgac gcgcacgacg ggcagcacca gcatcatcag ttcggtcacg gcaaacggat
    38041 gttctgcggt ggtttctaca aacagcagtc gcgtttgttc cggaacttta aggccgattg
    38101 ccgccgcgat tttcgccgca tcgcgaccaa cccagtcacg gctgacggtg ccttttccgc
    38161 gctcgtcgat atttttcagc aacaccgatt gcagttgctg cgcctgctct gcggtcagtt
    38221 tcaccgcatg ctggccttcc atcagacgca tcagttcatc ggctacgcta tcaacaacaa
    38281 tcagtacctt ttcgtcggca caaatgatgt tgttatcgaa agaagcgcct ttgacgatgg
    38341 actgagcagc acgggcgagg tcggcggttt catccaccac taccggcggg ttgccagcgc
    38401 ctgcggcaat cagacgttta ttggtgtgtt tacgcgccgc ttctactacc gcttcgccgc
    38461 cggtcaccac caaaaggccg atgcccggaa acttgaacaa gcgttgcgcg gtttcgatat
    38521 ccggatttgt tacggtaacc agtaagtttt ccggcccgcc tgcggcaaca atcgccaggt
    38581 tgagcagcgt aatcgcccgc tgggagactt ttttcgccgc cggatgcggg gcaaaaatga
    38641 cgctgttgcc cgcggcaatc aggctgatgg cgttgttaat tacggttgcc gccgggttag
    38701 tggaaggcgt cactgaagcc accacgcccc agggtgcgtt ttcaatcagg gtcaggccgt
    38761 tgtcgccagt cagcacttgc ggagagaggc actcaacgcc tggtgtgccg cgcgcctgag
    38821 cgacgttttt ggcaaattta tcttcaacgc gccccatgcc ggtttcactg acggcaagtt
    38881 ccgctaaatc tctggcgtgt ttttcgcctg cttcacgaat ggcagcaatg gctaactggc
    38941 gcattgccac gctttttaac ccttgctggg cgactttggc tgccgcaacg gcgtcatcca
    39001 gggacgcgaa aacgcccatc tcatgaacgg cggcgggcgg cgtgtcactg ctttgcattt
    39061 tcagcagtac cgctttcacc acctgttcaa tatcctgttg attcatgatg ttctgcctta
    39121 tttgtggaaa atgacctgac cgccagacac cacctcatcg acaatgccaa tcacgcacag
    39181 atcgaccggt gacgtttcgc ttttatgcgc ctggcgggcg gaactgccac tcaccagtaa
    39241 cacccactcc ccggttcccg cgccaatatt gtcgatggcg acggcgcatt gcccgtcagg
    39301 attaccttgt ggatcaatca tttccaccat cagcaatttg tcatgcgcca gtccgtgatg
    39361 gcgtacggta caaacaattt gtccagtgac gactgccagt ttcatacccg cctccgtggc
    39421 gtatttcagg taaaagctcc ccctaccctc cgcagaaggt aaaatgaaaa aggagagagc
    39481 gtgacgcccg aatcgacgtc acacagggtg attacaggtt gctgctatcg cctttcaggc
    39541 cgatcgggaa gacttcttcc aggtcgccgt gcggacgcgg gataacgtgt acagatacca
    39601 gctcgccgat acgctgtgcc gctgcggcac ctgcatcggt cgcggcttta caggctgcaa
    39661 catcaccacg aaccatggca gtacacaggc cgccgccaat ctgcttaaca ccaaccagct
    39721 tgacgcgcgc agctttaacc atcgcgtcag aagcctcaat cagtgcaacc aggccccggg
    39781 tttcgatcat tcctaatgct tccattgtgc tttcctcttt atcagggtcc agaacgggac
    39841 cgttcattca accagtgttt gtaaactgct ttcgcggttc acttctgtct gacgcggcac
    39901 agctgccacc agcgccagtt cgataatttc ctgcacgcta caaccacgag agagatcgtg
    39961 catcggcgcg gcaagtcctt gtatcaatgg cccgacggca cgatatccgc caagtcgttg
    40021 tgcgattttg tagccaatat ttccggcttc cagcgacgga aaaaccatca cattagcttt
    40081 gccctgtaac gggctggcag gcgctttttg cgccgccact tccggcacga aggcggcgtc
    40141 aaactgcaac tcgccatcga caaccagctt tggtgcgcgc tcacggacga tttctgtcgc
    40201 ctgctggacg ttggcaacac aggggtgacg ggcactaccg ttgctggaaa acgacagcat
    40261 cgccacgcgc ggttcttctc cggtgatggc gcgccaggtt tcggcgctgg caagcgcgat
    40321 atccgccagt tgtgccgccg tcggctgtgg caccacgctg caatcggcaa aacccaacgc
    40381 cggaccgctg tactgtggca gcatcaggaa aatggatgag agcgttttac agcctggctg
    40441 caagccgata atgcgtaatc cggcacgcag cacattcgcc gtggaagaga ggttgcccgc
    40501 gatacagaca tccgctttac cggcgctgac cattgcagcg gcgaacatca gcggatcggt
    40561 aagtttttcc agcgcatccg gcggcgtttt ttcgcccgcg cgggccagcc agcgatgagc
    40621 aaattcttcc cgcattgcga ggttgccatg cggatctatc acctgtagcc cgtccatcgc
    40681 cacgccgtga ctgagcgcaa actgacgaag ttcaaacgga ttggcgacca gaatgggcgt
    40741 tgccagacct tgttgatgta aatattgcgc agctttcagc acacgttgat ctaacgcatc
    40801 cggaaaaact actctggcgg gcgctcgcaa cgccagttca cgacaacgtt taataatcat
    40861 tgcgtttccc ccagtcgttg ttggatctgc ttcaccgtta gcggctgctt cgttacgctc
    40921 aggatcatca tcacgtagac cgtgctggaa aggcggttca gcgcctgcaa aatgtccggg
    40981 cgtaatactt caaagctgcg ggtgataaag acctgcgccg ctacggtttc tgtttcccgc
    41041 actttggtgc gtagcagatt gagcaacgcc gcatcgcgcc cgtggctggc ttccggcacc
    41101 agatgatcgt ggtcgagata acgcagcggc tggtgagaaa gccgatgcag atcttcgtca
    41161 gaaagcccga caatcgcttg ctcgcccagc ggttccccca gcgcatcggc acgcataatg
    41221 ttgccaagac gcgagcggat gtccgccagc cacggctgcc acggttctgc cagttcaatt
    41281 tgcagccaca ctgccagcgc aatggtgctg tcgagaacag cgcgaaaacc cagacgcgga
    41341 tcgcttttgg caaccatttt ttccgccgac aggtgagtca gcgtatccgg cttttttgcc
    41401 accggctggc ggcacagttc gcagcacgcc tgcggatgtt catcgctact ggtcagcccg
    41461 tgaacgggct gcggctgctg ctgttcatcg tcaacaaaca ggcggccctg ctcgtcaata
    41521 aacttgatgc gcagatggcg gctttccagt aactcccggg cagagggcgt caggcgactg
    41581 tccgcaggga gatggatctc tgcgccttcg ctgagcgtat ggttcgctct tagccatgct
    41641 tcggtgatga aatctttcat aaggattgcc agttagccgg ccaggcgaca tacaggaatt
    41701 tcacgctgga tgtcgtacca aattcgatgc tggagccttt cgggataaac atcacatcgc
    41761 ccgctttggc gatcatagtt tcgccttcgt ggcgaacatg cagctcccct tccagcacca
    41821 tgtcgatttc gtcgtagttc agggtccacg gaaagaaggc gttctcccac tgcatgaaac
    41881 cagcggccat actgctgccg tcgtctccgg tcacgagatc agtcaagcct acgcagtgcg
    41941 gttccgcgcc atcgaaacga ccaaacttga cgctgctgcc atcaataact ttgatgccgc
    42001 ctttgccggt caccgattta aagctcggct gcagtgcacc ctgttccagc gactgttttt
    42061 ccttcatcac tttttccatc agctgcgcga ccaggctttc ggtaaactga ccttccggca
    42121 gttgggcgat aatggtttca cggatgcgct ggctttcggt tttatcggct gatgcgctgg
    42181 cgggaacaga cgccgttacc gggatcgatt catcacattc ggtgatggta aaacccaaca
    42241 gatcagccac ttcacgagct tccggggtaa taatgctggc gcgcagaacc acggacattg
    42301 ccagttcgcc gcgcgcgtgt gcttcacgaa tatcattcgc tgtgatgagt tttttcacct
    42361 gcttcctcct gtttggtgag agatgccaga taatccacca gttgctgcac gctttgcggg
    42421 tcgtggctgt ttagctcgaa aatcggctct tcgaagcccg tctcaagcaa taactttcgc
    42481 gttgcggcga cgtcagcatc tggcatgtcc gttttactga tgacggcgat ttgccgtttg
    42541 ctgacgccaa tatccagcaa cccggcaggc aaccgacttt ccgggtcatt cgcgccgtgg
    42601 acatagatca gcatatccac atcctgtagc gtggtaatta aggcgtgata ccagcgggga
    42661 tggctaaaat attcacccgg cgtatcaata tcgccattat cattaaattc cacggcctgt
    42721 gtttttcttg cgagggtata attcccctgc aacgcattaa ataacgttgt ttttcctgcc
    42781 ccgacggtac ccacaaacgc aattcgtttc atgaatgcct cgtattaact tttggtcatt
    42841 tcacaaagcg tataatttaa taagcgtcct aaaccactga cggtttgtga taacgcttct
    42901 tctactgcgc ccaccgagcc ataaatcacc agcgcgccac taaaacgatc gagaaaaccg
    42961 atatgcacat cggcggcttt caacgctaaa tcaccggcga tcatcgccgt ttctccggga
    43021 gttagcgtca taatgccgat tgcgcccgca tccggaacgc cgattttctt cgccagttcc
    43081 tcgccgggat gcgcgatgag atgcgccagt gtgacctgtt taccgggcac aaattcctga
    43141 atgatgcgtt ctttatccat gagtcgccgc ctgtgaaaaa ctttcgtgta tcgtgacaat
    43201 ttttcgtcag agtaaataac aaaattcggc aagcgcgttt aaaaggtgag gtagatcact
    43261 aagaatggag agatatttgc tgtggtgcat aaattttcat atacaacgca tgaatataaa
    43321 taaaataata tcgagatatt ttatatactg atttttttat tattcgcaaa ctcagtaata
    43381 aactttacta atatattgca gctaaaccgc ttaccctgaa tattcagggt aagcgtgaga
    43441 gttaaaaaag aattacagcg gttgggtttg cgcttcaacc acggccagcg ccaccatgtt
    43501 gacgatacga cgtaccgatg cgatcggcgt taacacgtga accggcttcg ccacccccat
    43561 cagcaccggg ccgacagtca caccttccga gctggaaaca cgcagtaagt tgtaactaat
    43621 gcgggcagct tccatgttcg gcatcaccag aatattagcg gaacctttca aagggctgtc
    43681 cggcatacgg tcgttgcgaa tcgcttccac cagcgctgca tcgccgtgca tttcaccatc
    43741 aatcatcagt tctggcgcac gttccctgac cagttccagc gcctgacgca ttttgctcga
    43801 cgacgggcag tcagaagaac caaagttgga gtgcgacaac agagccacac gcggctcaat
    43861 accaaaacga cgaacagttt ctgccgccat caaggtgatc tccgccagct cttctgcatc
    43921 aggctcatca ttgacgtagg tatcggcaat aaaggtgtta ccactcggca acagcagcgc
    43981 gttcattgca cctgcggtgt gaacgccatc gcgataacca aagacatttt tcaccacgct
    44041 aaagtgttca tgataatcgc ccaccgtacc gcaaatcatt gcatcggctt cgccacgctg
    44101 aaccatgatc gcgccgatca ctgtcgggtt actgatcagc gcacgctgcg cctgctcctg
    44161 agtgacaccg cgacgcttca tgatctggaa gtattcggtc cagtactcct taaagcgcgg
    44221 atcggattcg ttattgacga tctcaaaatc aacgcccgcc ttgatctgca agcccagttt
    44281 ctgaatgcgc atttcgatca cgttcggacg accaataagg atcggtttcg ccagtcccag
    44341 cgttaccagt tcctgagtgg catgtagaac gcgcgcctct tccccttccg gcagaactac
    44401 gcgcttcggc gctttgcgag cctgggagaa aatcggcttc ataaacaggt tggttttgta
    44461 aacgaactcc gtcagcttgt cgatgtagac gtcgaaatca gcaatcggac gcgtcgccac
    44521 gcccgactcc atcgccgctt tcgccaccgc aggagcgatc ttaacgatca agcgcggatc
    44581 aaacggtttt ggaatgatgt attccggacc aaagctcaga tcctgatcgc catacgctga
    44641 agccaccact tcactctgtt ccgcatgggc cagttctgca atcgcgcgta ccgccgccag
    44701 tttcatctct tcgttgatgg cggttgcgcc aacgtccagc gcgccacgga agataaacgg
    44761 gaagcaaagg acgttgttca cctggttcgg gtagtcggaa cgaccggtac agatgatggc
    44821 atccggacgc acttctttcg ccagcggcgg cagaatttcc ggttccgggt tcgccagcgc
    44881 caggatcatt ggcgcacggg ccattttctt caccatttcc tgggtcagca ctttcgggcc
    44941 ggaacagccg aggaaaatat ccgcgccttc aatcacatca tcgagggttc gtttgccgtc
    45001 atccaccacc gcatatgcgg ctttggtttc cgccatgttt ggctcacggc cctgatagat
    45061 aacgcctttt gaatcgcaaa ccacgatgtt atgtttttgc agacccagcg ctaccagcag
    45121 gttcatacag gcgattgctg cggcacccgc gccagaaacc accatccgca cgtcggagat
    45181 gtttttctcc accacgcgca agccgttgag gatggcggca gtgctgataa ttgccgtgcc
    45241 gtgctgatcg tcgtggaata ccggaatatt catccgctcg cgcagtttct gttcaatata
    45301 gaaacattct ggcgctttaa tgtcttcgag gttgatgccg ccgaaggtcg gttcgagcgc
    45361 agcgacgact tcaataaatt tgtccgggtc gagttcatca acttcaatgt caaatacatc
    45421 aatcccggcg aatttcttaa acagaacgcc cttgccttcc atcaccggtt tgcctgccag
    45481 cgcgccaatg ttgcctaacc ccagcaccgc cgtaccgtta gagatcaccg ctaccaggtt
    45541 accgcgcgcg gtatatttgt aggcttttaa cgggtctttt tcgatttcaa gacaaggtgc
    45601 ggcaacgcct ggtgagtagg ccagcgccag atcgcgctgc gttgccagag gcttggttgg
    45661 agaaacctgg attttccctg gaactggaaa ttcatggaaa tcaagtgcac tttgttttaa
    45721 ctggtcatcc atttggttgt tcctttcacg taacgttcac aaataaattg tgtgggcaac
    45781 agcccctgcc cacaacgtgg cgcacattat taccctgccg gagtctacag actttgagca
    45841 agtccaaact ctcaccatta atataatgtt ttggtaataa tcatataaca ctgatgttac
    45901 ctgcttaatc cagcaatacc atgcctgtct gctatgcttt tttgatgcgt ttagcgaaat
    45961 ttctcagaag tgtgaattaa cgcactcatc taacacttta cttttcaagg agtatttcct
    46021 atgaacgagt tagacggcat caaacagttc accactgtcg tggcagacag cggcgatatt
    46081 gagtccattc gccattatca tccccaggat gccaccacca atccttcgct gttactcaag
    46141 gctgccggat tatcacaata tgagcattta atagacgatg ctatcgcctg gggtaaaaaa
    46201 aatggcaaga cccaggaaca acaggtggtc gcagcgtgtg acaaactggc ggtcaatttc
    46261 ggtgctgaaa ttctcaaaat cgtacccggt cgcgtgtcaa cagaagttga tgcacgcctc
    46321 tcttttgata aagaaaagag tattgagaag gcgcgccatc tggtggattt gtatcagcaa
    46381 caaggcgttg agaaatcgcg cattctgatc aagctggctt cgacctggga aggaattcgc
    46441 gcggcagaag agctggaaaa agaaggtatt aactgcaacc tgacgctgct gttttctttt
    46501 gcacaggcgc gggcctgtgc agaagcaggc gtttttctga tttcgccatt tgtcgggcgt
    46561 atttatgact ggtatcaggc acgcaagccg atggacccgt atgtggtgga agaagatcca
    46621 ggcgttaaat cagtacgcaa tatctacgac tactataagc aacaccacta tgaaaccatt
    46681 gtgatgggcg cgagcttccg tcgcaccgaa caaatcctcg ccttaaccgg ctgcgaccga
    46741 ctgactatcg caccgaattt gctgaaggag ctacaggaaa aagtttcgcc agtggtacgt
    46801 aaattaatcc caccttctca gacgttccca cgcccagctc ccatgagcga agcagagttc
    46861 cgttgggagc acaatcagga tgcgatggcg gtagaaaaac tgtctgaagg cattcgtctg
    46921 ttcgccgttg atcaacgcaa actggaagat cttcttgccg ccaaactata aaccagccac
    46981 ggagtgttat atgtcccgaa aagaccttgc caatgcgatt cgcgcactca gtatggatgc
    47041 ggtacaaaaa gccaattctg gtcatcccgg cgcgccgatg ggcatggctg atattgccga
    47101 agtgctgtgg aacgattttc ttaaacataa ccctaccgac ccaacctggt atgatcgcga
    47161 ccgctttatt ctttccaacg gtcacgcgtc gatgctgctc tacagtttgc tgcatctgac
    47221 cggttacgac ctgccactgg aagaactgaa gaacttccgt cagttgcatt cgaaaacccc
    47281 tggtcacccg gaaatcggtt atacgccagg tgttgaaacc accaccggtc ctcttggaca
    47341 aggtttggcg aacgccgtcg ggctggcgat agcagagcgt acgctggcgg cgcaatttaa
    47401 tcagccggat catgaaattg tcgatcactt cacctatgtg tttatgggcg acggctgcct
    47461 gatggaaggt atctctcacg aagtctgttc gctggcgggc acgctggggc tgggcaagct
    47521 gattggtttt tacgatcaca acggtatttc catcgacggt gaaaccgaag gctggtttac
    47581 cgacgatacg gcaaaacgtt ttgaagccta tcactggcat gtggtccacg agatcgacgg
    47641 ccacgatccg caggcggtga aggaagcgat ccttgaagcg caaagcgtga aagataagcc
    47701 gtcgctgatt atctgccgta cggtgattgg ctttggttcg ccgaataaag caggtaagga
    47761 agaggcgcac ggcgcaccgc tgggggaaga agaagtagct ctggcacggc aaaaactggg
    47821 ctggcaccat ccgccatttg agatccctaa agagatttat cacgcctggg atgcccgcga
    47881 aaaaggcgaa aaagcgcagc agagctggaa tgagaagttt gccgcctata aaaaggctca
    47941 tccgcaactg gcagaagagt ttacccgtcg gatgagcggc ggtttgccga aggactggga
    48001 gaaaacgact cagaaatata tcaatgagtt gcaggcgaat ccggcgaaaa tcgctacccg
    48061 taaggcttcg caaaatacgc ttaacgctta cgggccgatg ctgcctgagt tgctcggcgg
    48121 ttcggcggat ctggctccca gcaacctgac catctggaaa ggttctgttt cgctgaagga
    48181 agatccggcg ggcaactaca ttcactacgg ggtgcgtgaa tttggtatga ccgctatcgc
    48241 caacggcatc gcgcaccacg gcggctttgt gccgtatacc gcgacgttcc tgatgtttgt
    48301 tgaatacgcc cgtaacgccg cgcggatggc ggcactgatg aaagcgcggc agattatggt
    48361 ttatacccac gactcaattg gtctgggcga agatggtccg acgcaccagg ctgttgagca
    48421 actggctagt ctgcgcttaa cgccaaattt cagcacctgg cgaccgtgcg atcaggtgga
    48481 agcggcggtt ggctggaagc tggcggttga gcgccacaac ggaccgacag cactgatcct
    48541 ctccaggcag aatctggcac aggtagaacg aacaccggat caggttaaag agattgctcg
    48601 tggcggttat gtgctgaaag acagcggcgg taagccagat attattctga ttgccaccgg
    48661 ttcagagatg gaaatcaccc tgcaagcggc agagaaatta gcgggagaag gtcgcaatgt
    48721 tcgcgtagtt tccctgccct cgaccgatat tttcgatgcc caggatgaga aatatcggga
    48781 gtcggtactg ccttcaaatg tttctgcccg cgtggcagta gaagcgggta ttgccgatta
    48841 ctggtacaaa tatgttggcc tgaaaggggc aattgtcggg atgacaggtt atggggaatc
    48901 agctccggcg gataagctgt tcccgttctt tggctttacc gccgagaata ttgtggcaaa
    48961 agcgcataag gtactgggag tgaaaggtgc ctgatggtga tcgccggatg ctgattgtcg
    49021 gatgtgacgc tggtgcgtct tatccggtct acatgtcccc gccattttgt ttaacgggtg
    49081 atccacaacg tgggccaggc gtctggccca tgccagttat cgcaggtggg ttctgcggcg
    49141 taacgcacca ggcgaaaacg ctgaccgtca aagcgccagc gcgcctgaat gccacaatcg
    49201 cttaatccgc gcccttttgc taaggtcacc agttcacgcg atttttcatc gaatgttgcg
    49261 ttcatcagtt ccagttcatt ggtctcctga ccgttgttga acggcaaacg caaccgtacc
    49321 gggcgcgagg cctgcggctt tttacgcgac acaatccatg ccaaatcaat ggtgttatag
    49381 gcccctgcct cacagctaat catcatcagc gctttatcat cggtcagcgc agtgacattc
    49441 acctcacgac gtaacggatc aagcgaacag cgcagaccat tcatccgcca gtttccataa
    49501 tccagcaaat cgttacgttc ttcgagtgag agtggcgtcg gcgttgggtt aaccaccgcg
    49561 acctctttca gcgcaggcgc tggcggtacg ctgagcggcg gttcgtcccc tttctttatc
    49621 cacgcggtct cactgccaac gcgcttttgc tgggcatcaa taaacaacaa cgccgctttt
    49681 aagccactca gagaaatagt ctgattgccg tcgcgtaagg tgatcgccct cccttcctga
    49741 atcatctgca aaaacgcggt gatggttgcc gtatcatcgg ttactaataa ccatggtgaa
    49801 atccgccact tgtcaccact taacgccaac ggctcaccat ctaacagcag ccgtggcgct
    49861 atctcccctt ctgacgcatc cggcgacttc aacccgccgc gctcaatacg taaaacggca
    49921 tcggtatgcg ccccggcgct gcggctcagg gtcatcacca gtccattatg atcgcccgta
    49981 ttgcgcgcta cacagaaatt ttgattatta caggtgacct gccagtcgga aaacgcccgt
    50041 tgcgctggtg ccgcccacac caaagacatc ggcaacaaag cgaaaaaaaa gagaaggaaa
    50101 atgcgatatc gcatggacgg tacgacccca gaagttcaaa caaacagcac aagtcgtatc
    50161 ttcgttgcgc aggtagcgcg gctcaatcgg atttgtcgga ttgagccaat aataaacaac
    50221 gaaatattat tgttattttt cagtccatta aatgtgacat ttgcaaatag ttaagcaata
    50281 acaccgtctt accgtcacgt atctcgccgg ttttaatcat ctccagcgcc tggctgaacg
    50341 gcagctcaag cacttcaata tcttcatctt cgacaccacc accggcgtta gcgcgctggc
    50401 tgtcactgta ttcggcgata aaaaagtgga ttagctcagt cacaccgcct ggcgacatat
    50461 acagttcaaa taatttgcgg acttcgccaa cttcatagcc cgtctcttca atcgcttctt
    50521 tgcgaatgca cacttccggt tcgtcgttat ccagcagccc ggcgcaggtt tcaatcagtt
    50581 gcccgctttc attgccatta acccaggtag cgacgcggaa ctggcgaatc agaaccacgg
    50641 tctttttctt cgcgttgtac aggaggatcg tcgcaccatt accgcgatcg taaacttcac
    50701 gtttatggcg gataacttcg ccatctttgc gcgtgagatc gtaagtaatg ttgtgcaggg
    50761 tgaaatagtt atcggagaga attttgtctt taacgagggt gatttgttgc gtcataccga
    50821 ctccacagcg cgaaatgaac aattatctta cgctgtgaag tcgggtttgt ctgcgcaggc
    50881 tatcagtgag atttgactga ttttacgccc agccagtcaa tgatcccctg tgccgcatga
    50941 cgtccttctg ccattgcggt aaccaccaga tccgcaccac gcacggcgtc accaccggcg
    51001 aagattttcg gattcgtggt ctggtaacgg tactggcttt ccacatccgc gatgatgcgg
    51061 ccccatttgt ctaccgttac accgtgcgac tccagccacg gcatcccgtg cggattgaag
    51121 ccaaacgcca taatcaccgc atcggctggc atgacaaatt cactgccttc caccggcact
    51181 ggacgccgac gtccctgggc atccggctcg ccaagacgcg tgcgcaggaa acgaatcccg
    51241 cagacgtgac cttgttcatt cagctcaagc gccaccggct ggacgttaaa ttcgaagttg
    51301 gccccctctt cacgggcgtt cttcacctct ttcttcgagc ctggcatgtt agcttcatca
    51361 cgacgataag cgcaggtgac gttactcgcg ccatggcgca gcgcggtacg cacacagtcc
    51421 atcgcggtgt cgccgccgcc cagtaccacg acgttaagtc cggcggtatt gataaacggc
    51481 tcttccggta gctcttcgag tcccatcacc tgtttagtgt tggcgatgag gaacggcagc
    51541 gcgtcataaa cgcccggcgc atcttcattg ggtaaacccg ctttcatgga acggtaagtg
    51601 cctacgccaa cgaagaccgc gtcgtattgt tccaaaagcg aattcaaaga gacatcttta
    51661 cccacttcac aattgagttc gaagtgaatc cccatcgcgc tgaagatttc ccgacggcgt
    51721 gccagcaggg atttatccag tttgaaagaa ggaatgccga aagtgagcaa gccaccgatt
    51781 tctggatggc gatcgtacac cgtcaccgcc acgccattgc gggtcagaac atctgcacag
    51841 gccagccctg ccggacccgc accgataatc gccacccgct tgtccacttt ggtgacatgg
    51901 cttaagtcag gacgccaacc tttcgccaac gcctgatctg aaatgtagcg ttcaatgtta
    51961 ccgatagtta ctgcgccgtg ctcatcgcga atagtacagg caccttcaca caaacggtct
    52021 tgcgggcaaa cgcgtccggt aatttccggc agggtgttgg tctggtgaga aagctcgacg
    52081 gcggcgtcga tgtttccggc tttcaccagt tcaatccact gcggtatatg gttatgcagc
    52141 gggcaggtcc attcacaaac gctgtgctcg ccgcacttaa ggcagcgcga ggcttcccgt
    52201 tgtgcctgat cggcacggaa tggcagataa atttcatcaa aaccggtttt gcgcgcttca
    52261 atcgccagtt tatctggctc gccacgcgcg ggcgttgcct gcatttgctc gactttactc
    52321 attaccggca tttcttgcgc cgcggtactg gcatgccacg gttgatgttc ctgacgtgcg
    52381 gtgcgcaagc ggcgggattt cgccatgccg gagagtgcgg cgtcagtgac cagttgcagc
    52441 gcgtccgccg ggcaattttc gacacaggca ggaccgtttt cgcgccctgc acaaaggtcg
    52501 catttatgcg ccgtggcttt tacttttcct gccgcaacgg gtgtcagaac gatttgcatc
    52561 gtaccaaatg gacaggccac cacgcaggat ttacagccaa tacacttttg ctgattgacc
    52621 tgaatgctgt catcaacgtg gctgattgcg ccattagggc agctacgggc gcagggcgca
    52681 tcttcacaat ggtgacaggt cacggcacta cgttgctgct gatgtttgat aaccgtaatt
    52741 cggggatgaa aatggtgttg gctcaggaca tgttgctcgt cattgtgagc catgacacag
    52801 gcgatttcac aagcatgaca acccagacat tgctgactgt tggccataat aaaacgattc
    52861 ataacgacct tcttttttgg ttgtaaaaac cttattcttt atatgagtgt tgttattacc
    52921 cgacttacag gggaatcggc aatgttcatg tgcccagaat aagtaactat ttcgctataa
    52981 actgtggcag atcaaataat cccatcactg actaaattgc gtttcagcga actggaagat
    53041 taatgatttt ttgtggagaa gacgcgtgtg attgttaaac gacccgtctc ggccagtctg
    53101 gcccgggcct ttttttacat tgtgctgctg tcgattcttt ccacgggtat cgctctgcta
    53161 acgctggcga gcagtttacg cgacgctgag gctatcaata ttgccggatc gctgcgtatg
    53221 cagagttacc gcctgggcta cgacttgcaa agtggcagtc cacaactcaa tgcacatcgc
    53281 cagttatttc agcaggcact gcattcaccg gtattaacca atctcaacgt ctggtatgtg
    53341 ccagaggcag taaaaacccg ctatgcgcat ctgaatgcca actggctgga gatgaataat
    53401 cggctcagca agggcgactt gccgtggtat caggccaata ttaataatta tgttaatcag
    53461 atagacctgt tcgtgctggc tttacagcac tacgctgaac gcaaaatgct gctggtggtg
    53521 gcaatttccc tggctggcgg catcggtatt ttcacgctgg tcttttttac tttgcgccgc
    53581 atacgccatc aggtggttgc cccgctgaat cagctggtta ccgccagtca gcgtattgaa
    53641 cacgggcagt tcgactcgcc gccgctggat accagcctgc cgaatgagct tggcctgctt
    53701 gcaaaaacct ttaaccagat gtcgagcgag ctgcataaat tgtaccgttc gctggaggcg
    53761 tcagtagaag aaaagacccg cgatctccac gaggccaagc gtcgtctgga ggtgttgtat
    53821 cagtgttcgc aggcgctaaa caccagccag attgatgtgc attgtttccg ccatattttg
    53881 cagattgttc gcgacaatga agcggctgaa tatctggagt taaatgtcgg tgacaactgg
    53941 cggattagcg aagggcagcc aaacccggaa ttgccgatgc agattttacc ggtgacaatg
    54001 caagagacgg tttacggaga actgcactgg caaaatagtc acgtttcatc atcagaaccg
    54061 ctgcttaaca gcgtttcgtc gatgctggga cgcggtttgt actttaatca ggcgcagaag
    54121 cattttcagc agttattgtt gatggaagaa cgtgcgacca tcgcccgcga attgcacgac
    54181 tcgctggctc aggtactttc ttacttacgt atccagttga cgttactgaa gcgttcgata
    54241 ccggaagata acgccaccgc acaaagtatc atggccgatt tttcccaggc gttgaatgat
    54301 gcttatcggc agttacgcga gctgttgact actttccgcc tgacgctgca gcaggcggat
    54361 ctcccctccg cgttgaggga aatgctggat acgttacaaa atcaaaccag cgccaaactg
    54421 accctcgact gccgtctgcc aaccctggcg ctggatgcgc aaatgcaggt gcatttgttg
    54481 caaattattc gcgaagcggt gctgaatgcg atgaagcacg ccaacgccag cgaaatcgcc
    54541 gttagctgcg tcaccgcgcc ggatggcaat cacacagtct atattcgcga caacgggatt
    54601 ggcatcggtg aaccgaaaga acccgaaggc cattatggtc tgaatatcat gcgcgaacgc
    54661 gcggaaagat tgggtgggac gctgactttt tcacaacctt ccggcggcgg cacgttagtg
    54721 agtattagct ttcgctctgc ggagggtgag gaaagtcagc tgatgtaatg cctcttattg
    54781 accaaagaat agtggcactt aaggttcagt ataaaagggc atgataattt acattaactc
    54841 ctttttttct ccacgattgg ctcgtacctt gccgctacag tgaagcaagc caagcctaca
    54901 acgatacgca gaaacacgag gtcctctttt aatggcgaat ttctttattg atcgccccat
    54961 ttttgcctgg gtgctggcaa tcctgttgtg tctgacaggt accctggcga ttttttcatt
    55021 gcccgttgaa caataccccg atctcgcgcc accgaatgtg cgagtgaccg ctaactatcc
    55081 cggcgcatcg gcccagacgc tggaaaacac cgtgacccag gttatcgagc aaaatatgac
    55141 cggcctcgat aatctgatgt atatgtcatc tcagagcagc ggcaccggtc aggcatctgt
    55201 cacgttaagt tttaaagcag gcaccgatcc ggacgaagcc gtgcagcaag tacaaaacca
    55261 gctgcaatca gccatgcgaa agttaccgca ggcggtgcaa aatcagggcg tgacggtgcg
    55321 taaaaccggc gataccaaca ttctgaccat tgccttcgtc tctaccgatg gttcgatgga
    55381 taaacaggat attgccgatt atgttgccag taatattcag gacccgttaa gccgcgtgaa
    55441 tggcgtcggg gatatcgatg cctatggttc gcaatattcc atgcgtatct ggctggaccc
    55501 ggcgaaactc aacagtttcc agatgacggc taaagatgtc accgatgcca ttgaatcaca
    55561 gaacgcgcag attgcggttg ggcaacttgg tggtacgcct tccgtcgata agcaggcgct
    55621 caacgccacc attaacgccc agtcactgct gcaaacacca gaacagttcc gcgatatcac
    55681 cttgcgggtg aatcaggacg gctcagaggt aaggctgggc gatgtcgcca ccgtcgaaat
    55741 gggggcggag aaatacgatt atcttagccg cttcaatggt aagccagcct ccgggctggg
    55801 ggtaaaactg gcctccggcg cgaacgaaat ggcgacagcg gagctggtgc tcaatcgtct
    55861 cgacgagctg gcgcagtatt ttccacacgg actggaatac aaggtggcgt atgaaaccac
    55921 ctcttttgta aaagcctcca ttgaagacgt ggtgaaaacg cttctggaag ctatcgccct
    55981 ggttttcctc gttatgtatc tgttcctgca aaacttccgc gccacgctga tacccactat
    56041 cgccgtgccg gtggtgttga tgggaacctt ctcggtgctt tatgccttcg gctatagcgt
    56101 caacacctta accatgttcg cgatggtgct ggcgatcggt ctgctggtgg atgatgccat
    56161 cgtggtggtg gaaaacgtcg aacgtattat gagtgaggaa ggactcactc ctcgcgaagc
    56221 cacacgtaaa tcgatggggc agatccaggg ggcactggtc gggattgcga tggtgctttc
    56281 ggcggtattt gtgccaatgg ccttcttcgg cggcaccacc ggtgccatct atcgccagtt
    56341 ctctattacc attgttgcgg cgatggtgct gtcagtactg gtagcgatga tcctcactcc
    56401 ggctctgtgt gccacactac ttaagccact gaaaaaaggt gagcatcacg ggcaaaaagg
    56461 cttttttgcc tggtttaacc agatgtttaa ccgcaacgcc gaacgctacg aaaaaggggt
    56521 ggcgaaaatt ctccaccgta gcctgcgctg gattgtgatt tatgtcctgc tgcttggcgg
    56581 catggtgttc ctgttcctgc gtttgccgac gtcgttctta ccgctggaag accgtggcat
    56641 gtttactacc tcggtacagt tgcccagcgg ttcaacccag caacagaccc tgaaagtcgt
    56701 tgagcaaatc gagaaatatt acttcaccca tgaaaaagac aacatcatgt cggtgtttgc
    56761 caccgtgggt tctggccctg gggggaacgg gcaaaacgtg gcgcgaatgt ttatccgcct
    56821 gaaagactgg agcgaacgcg acagtaagac cggcacctcg tttgccatta tcgagcgtgc
    56881 aacgaaggcg tttaacaaga ttaaagaagc tcgcgttatc gccagcagcc cgccagcaat
    56941 tagcggtctt ggtagttctg cgggttttga tatggagttg caggaccacg ctggagcggg
    57001 tcacgatgcg ctgatggcag cacgaaacca gttgctggcg ctggcagcgg aaaacccgga
    57061 gctaacccgt gtgcgccata acggcctcga cgacagtccg cagttgcaga ttgatatcga
    57121 ccagcgtaaa gctcaggcgc tgggcgttgc tatcgacgat attaacgaca cactgcaaac
    57181 cgcctggggt tcgagctatg tgaatgactt tatggatcgc ggtcgcgtga agaaagtcta
    57241 tgtgcaggca gctgcgccgt atcgcatgct gccagatgac atcaatctct ggtatgtccg
    57301 aaataaagat ggcggcatgg tgcccttctc tgctttcgcg acctcacgct gggagacagg
    57361 ctcgccgcgt ctggaacgct ataacggtta ttctgcggtt gagattgttg gggaagccgc
    57421 accgggggtc agtaccggta cggcgatgga tattatggaa tcgttagtga agcagttgcc
    57481 aaacggcttt ggtctggagt ggacggcgat gtcgtatcag gagcggcttt ccggcgcgca
    57541 ggctccggcg ctgtacgcca tttccttgct ggtggtattc ctgtgtctgg ctgcgctgta
    57601 tgaaagctgg tcggtgccgt tctcggtaat gctggtcgtg ccgctggggg ttatcggcgc
    57661 gctgctggca acctggatgc gcgggctgga aaacgatgtt tacttccagg tgggcctgtt
    57721 aacggtcatt ggtttatcgg cgaaaaacgc catcctgatc gtcgagtttg ctaacgagat
    57781 gaaccaaaaa ggccacgacc tgtttgaagc gacgctccac gcctgccgtc agcgtttacg
    57841 cccgattctg atgacctcgc tggcatttat cttcggcgta ttgccaatgg caaccagcac
    57901 aggtgccggt tccggtggtc agcatgcggt gggtactggc gtaatgggcg ggatgatttc
    57961 ggccactatt ctggctattt acttcgtgcc gctgttcttt gtgctggtgc gccgccgctt
    58021 cccgctgaag ccgcgcccgg aataagcaat aaaaaaggcg acatgccaat gtgtcgccct
    58081 tttcaacttt ccgattaaga acctgctcag cgggttcttg ctgtttgtac tttgtctcag
    58141 gaattactta cgaagcataa cttcgataaa gtctttccag ttccccagtt cacgttcaat
    58201 cataacaacc tctcttataa ttatgggtat tctacggaaa caatataccg tggtgaagct
    58261 aatttactcg attgctgcga tgactacctc cgggggacaa accttatgta aatactatgg
    58321 tcctgcggtg atgatttgta tgtgatacac cgcaacattt cgagatattc atacggcatc
    58381 taatactgat ttaattctgg ttaaaataca gacagataac aagatgaata t
//
LOCUS       DAHHTG010000010        58396 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.010, whole genome shotgun sequence.
ACCESSION   DAHHTG010000010 DAHHTG010000000
VERSION     DAHHTG010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 58396)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 58396)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..58396
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.010"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            complement(<1..211)
                     /locus_tag="NBI74_000648"
     CDS             complement(<1..211)
                     /locus_tag="NBI74_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001544841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HCD1931024.1"
                     /translation="MALNCPTCKTPLERNGETAHCATCDKDFTVQALCPECHQPLQVL
                     KACGAVDYFCQHGHGLISKKRVEFVI"
     gene            complement(274..1746)
                     /gene="der"
                     /locus_tag="NBI74_000649"
     CDS             complement(274..1746)
                     /gene="der"
                     /locus_tag="NBI74_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HCD1931025.1"
                     /translation="MVPVVALVGRPNVGKSTLFNRLTRTRDALVADFPGLTRDRKYGR
                     AEIEGREFICIDTGGIDGTEDGVETRMAEQSLLAIEEADVVLFMVDARAGLMPADEAI
                     AKHLRSREKPTFLVANKTDGLDPDQAVVDFYALGLGEIYPIAASHGRGVLSLLEHVLL
                     PWMEDLAPQEEVDEDAEYWAQFEAEENGEEEEEDDFDPQSLPIKLAIVGRPNVGKSTL
                     TNRILGEERVVVYDMPGTTRDSIYIPMERDGREYVLIDTAGVRKRGKITDAVEKFSVI
                     KTLQAIEDANVVMLVIDAREGISDQDLSLLGFILNSGRSLVIVVNKWDGLSQEVKEQV
                     KETLDFRLGFIDFARVHFISALHGSGVGNLFESVREAYDSSTRRVGTSMLTRIMTMAV
                     EDHQPPLVRGRRVKLKYAHAGGYNPPIVVIHGNQVKDLPDSYKRYLMNYFRKSLDVMG
                     SPIRIQFKEGENPYANKRNTLTPTQMRKRKRLMKHIKKSK"
     gene            complement(1864..3042)
                     /gene="bamB"
                     /locus_tag="NBI74_000650"
     CDS             complement(1864..3042)
                     /gene="bamB"
                     /locus_tag="NBI74_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB"
                     /protein_id="HCD1931026.1"
                     /translation="MQLRKLLLPGLLSVTLLSGCSLFNSEEDVVKMSPLPTVENQFTP
                     TTVWSTSVGSGIGNFYSNLHPALADNVVYAADRAGLVKALNADDGKEIWSVNLAEKDG
                     WFSKDPALLSGGVTVSGGHVYIGTEKAQVYALNTSDGTVAWQTKVAGEALSRPVVSDG
                     LVLIHTSNGQLQALNEADGAVKWTVNLDMPSLSLRGESAPATAFGAAVVGGDNGRVSA
                     VLMEQGQMIWQQRISQATGSTEIDRLSDVDTTPVVVNGVVFALAYNGNLTALDLRSGQ
                     IMWKRELGSVNDFIVDGNRIYLVDQNDRVMALTIDGGVTLWTQSDLLHRLLTSPVLYN
                     GNLVVGDSEGYLHWINVEDGRFVAQQKVDSSGFQTEPVAADGKLLIQAKDGTVYSITR
                     "
     gene            complement(3053..3673)
                     /gene="yfgM"
                     /locus_tag="NBI74_000651"
     CDS             complement(3053..3673)
                     /gene="yfgM"
                     /locus_tag="NBI74_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ancillary SecYEG translocon subunit YfgM"
                     /protein_id="HCD1931027.1"
                     /translation="MEIYENENDQVEAVKRFFAENGKALAVGVILGVGALIGWRYWNS
                     HQVDSARSASLAYQNAVTAVSEGKPDSIPAAEKFAAENKNTYGALASLELAQQFVDKN
                     ELEKAAAQLQQGLADTSDENLKAVINLRLARVQVQLKQADAALKNLDAIKGEGWAAIV
                     ADLRGEALLSKGDKQGARSAWEAGVKSDVTPALSEMMQMKINNLSI"
     gene            complement(3691..4965)
                     /gene="hisS"
                     /locus_tag="NBI74_000652"
     CDS             complement(3691..4965)
                     /gene="hisS"
                     /locus_tag="NBI74_000652"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HCD1931028.1"
                     /translation="MAKNIQAIRGMNDYLPGETAIWQRIEGTLKNVLGSYGYSEIRLP
                     IVEQTPLFKRAIGEVTDVVEKEMYTFEDRNGDSLTLRPEGTAGCVRAGIEHGLLYNQE
                     QRLWYIGPMFRHERPQKGRYRQFHQLGCEVFGLQGPDIDAELIMLTARWWRALGISEH
                     VTLELNSIGSLEARANYRDALVAFLEQHKEKLDEDCKRRMYTNPLRVLDSKNPEVQVL
                     LNDAPALGDYLDEESREHFAGLCKLLESAGIAYTVNQRLVRGLDYYNRTVFEWVTNSL
                     GSQGTVCAGGRYDGLVEQLGGRATPAVGFAMGLERLVLLVQAVNPEFKADPVVDIYLV
                     ASGADTQSAAMALAERLRDELPGVKLMTNHGGGNFKKQFARADKWGARVAVVLGESEV
                     ANGTAVVKDLRSGEQTAVAQDSVAAHLRTLLG"
     gene            complement(5076..6194)
                     /gene="ispG"
                     /locus_tag="NBI74_000653"
                     /gene_synonym="gcpE"
     CDS             complement(5076..6194)
                     /gene="ispG"
                     /locus_tag="NBI74_000653"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HCD1931029.1"
                     /translation="MHNQAPIQRRKSTRIYVGNVPIGDGAPIAVQSMTNTRTTDVEAT
                     VNQIKALERVGADIVRVSVPTMDAAEAFKLIKQQVNVPLVADIHFDYRIALKVAEYGV
                     DCLRINPGNIGNEERIRMVVDCARDKNIPIRIGVNAGSLEKDLQEKYGEPTPQALLES
                     AMRHVDHLDRLNFDQFKVSVKASDVFLAVESYRLLAKQIDQPLHLGITEAGGARSGAV
                     KSAIGLGLLLSEGIGDTLRVSLAADPVEEIKVGFDILKSLRIRSRGINFIACPTCSRQ
                     EFDVIGTVNALEQRLEDIITPMDVSIIGCVVNGPGEALVSTLGVTGGNKKSGLYEDGV
                     RKDRLDNNDMIDQLEARIRAKASQLDEARRIDVQQVEK"
     gene            complement(6221..7228)
                     /gene="rodZ"
                     /locus_tag="NBI74_000654"
     CDS             complement(6221..7228)
                     /gene="rodZ"
                     /locus_tag="NBI74_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytoskeleton protein RodZ"
                     /protein_id="HCD1931030.1"
                     /translation="MNTEATHDQNEALTTGARLRNAREQLGLSQQAVAERLCLKVSTV
                     RDIEEDKAPADLASTFLRGYIRSYARLVHIPEEELLPGLEKQAPLRAAKVAPMQSFSL
                     GKRRKKRDGWLMTFTWLVLFVVIGLSGAWWWQDHKAQQEEITTMADQSSAELNNNQSQ
                     SVPLDTSTTTDQAMATTPTSPVDTTATNTQTPAVTAPAPAVDPQQNAVVPPSQANVDT
                     AATPAPAATTTPDGAAPLPTDQAGVTTPAVDPNALVMNFTSDCWLEVTDATGKKLFSG
                     MQRKDGNLNLTGQAPYKLKIGAPAAVQIQYQGKPVDLSRFIRTNQVARLTLNAEQSPA
                     Q"
     gene            complement(7513..8667)
                     /gene="trmG/rlmN"
                     /locus_tag="NBI74_000655"
     CDS             complement(7513..8667)
                     /gene="trmG/rlmN"
                     /locus_tag="NBI74_000655"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA
                     large subunit methyltransferase RlmN"
                     /protein_id="HCD1931031.1"
                     /translation="MSEQLVTPENVTTKDGKINLLDLNRQQMREFFKDLGEKPFRADQ
                     VMKWMYHYCCDNFDEMTDINKVLRGKLKEVAEIRAPEVVEEQRSSDGTIKWAIAVGDQ
                     RVETVYIPEDDRATLCVSSQVGCALECKFCSTAQQGFNRNLRVSEIIGQVWRAAKIVG
                     AAKVTGQRPITNVVMMGMGEPLLNLNNVVPAMEIMLDDFGFGLSKRRVTLSTSGVVPA
                     LDKLGDMIDVALAISLHAPNDEIRDEIVPINKKYNIETFLAAVRRYLEKSNANQGRVT
                     IEYVMLDHVNDGTEHAHQLAELLKDTPCKINLIPWNPFPGAPYGRSSNSRIDRFSKVL
                     MSYGFTTIVRKTRGDDIDAACGQLAGDVIDRTKRTLRKRMQGEAIDIKAV"
     gene            complement(8817..9248)
                     /gene="ndk"
                     /locus_tag="NBI74_000656"
     CDS             complement(8817..9248)
                     /gene="ndk"
                     /locus_tag="NBI74_000656"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HCD1931032.1"
                     /translation="MAIERTFSIIKPNAVAKNVIGSIFARFEAAGFKIVGTKMLHLTV
                     EQARGFYAEHDGKPFFDGLVEFMTSGPIVVSVLEGENAVQRHRDLLGATNPANALAGT
                     LRADYADSLTENGTHGSDSVESAAREIAYFFGEGEVCPRTR"
     gene            complement(9397..11709)
                     /gene="pbpC"
                     /locus_tag="NBI74_000657"
     CDS             complement(9397..11709)
                     /gene="pbpC"
                     /locus_tag="NBI74_000657"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417014.1"
                     /note="penicillin-binding protein 1C; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase PbpC"
                     /protein_id="HCD1931033.1"
                     /translation="MPRLLTKRGCWIMLTAAPFIIILAAWAADKLWPLPLQEVNPARV
                     VVAQDGTPLWRFADADGIWRYPVTIEDVSPRYLEALINYEDRWFWKHPGVNPFSVARA
                     AWQDLTSGRVISGGSTLTMQVARLLDPHPKTFGGKIRQLWRALQLEWHLSKREILTLY
                     LNRAPFGGTLQGIGAASWAYLGKSPANLSYSEAAMLAVLPQAPSRLRPDRWPERAEAA
                     RNKVLERMAVQGVWSRERVKESREEPIWLAPRQMPQLAPLFSRMMLGKSKSDKIVTTL
                     DAGLQRRLEELAQNWKGRLPPRSSLAMIVVDHTDMRVRGWVGSVDLNDDSRFGHVDMV
                     NAIRSPGSVLKPFAYGLALDEGVIHPASLLQDVPRRTGDYRPGNFDSGFHGPISMSEA
                     LVRSLNLPAVQVLEAYGPKRFAAKLRNVGLPLYLPNGAAPNLSLILGGAGAKLEDMAA
                     AYTAFARHGKAGKLRLQPDDPLLERPLMSSGAAWIIRRIMADEAQPLPDGALPRVAPL
                     AWKTGTSYGYRDAWAIGVNARYVIGIWTGRPDGTPVVGQFGFASAVPLLNQVNNILLS
                     RSVNLPEDPRPDSVSRGVICWPGGQSLPEGDGNCRRRLATWLLDGSQPPTLLLPEQEG
                     INGIRFPIWLDENGKRVAADCPQARQEMINVWPLPLEPWLPASERRAVRLPPASTICP
                     PYGHDAQLPLQLTGVRDGAIIKRLPGAAEATLPLQSSGGAGERWWFLNGEPLTERGRN
                     VTLHLTDKGDYQLLVMDDVGQIATVKFVMQ"
     gene            complement(11710..16671)
                     /gene="yfhM"
                     /locus_tag="NBI74_000658"
     CDS             complement(11710..16671)
                     /gene="yfhM"
                     /locus_tag="NBI74_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha2-macroglobulin"
                     /protein_id="HCD1931034.1"
                     /translation="MKKLRVAACMLMLALAGCDNNDNAPTAVKKDAPSEVTKAASSEN
                     VSSAKLSAPERQKLAQQSAGKALTLLDLSEVQLDGAATLVLTFSIPLDPDQDFSRVIH
                     VVDKKSGKVDGAWELSDNLKELRLRHLEPKRDLIVTIGKEVKALNNATFSKDDEKTIT
                     TRDIQPSVGFASRGSLLPGKVVEGLPVMALNVNNVDVNFFRVKPESLPAFISQWEYRN
                     SLANWQSDKLLQMADLVYTGRFDLNPARNTREKLLLPLGDIKPLQQAGVYLAVMNQAG
                     RYDYSNPATLFTLSDIGVSAHRYHNRLDIFTQSLENGAALQGIEVSLLNEKGQTLTQA
                     TSDAQGHVQLENDKNAALLLARKNGQTTLLDLKLPALDLAEFNIAGAPGYSKQFFMFG
                     PRDLYRPGETVILNGLLRDADGKALPDQPIKLDVIKPDGQVLRSVVSQPENGLYHFTW
                     PLDSNAATGMWHIRANTGDNQYRMWDFHVEDFMPERMALNLTGEKTPLTPKDEVKFSV
                     VGYYLYGALANGNTLQGQLFLRPLREAVSALPGFEFGDIAAENLSRTLDEVQLTLDDK
                     GRGEVSTESQWKETHSPLQVIFQGSLLESGGRPVTRRAEQAIWPADALPGIRPQFASK
                     SVYDYRTDSTVKQPIVDEGSNAAFDIVYSDAQGVKKAVSGLQVRLIRERRDYYWNWSE
                     YEGWQSQFDQKDLIENEQTLDLKADETGKVSFPVEWGAYRLEVKAPNEAVSSVRFWAG
                     YSWQDNSDGGGAVRPDRVTLKLDKASYRPGDTIKLHIAAPTAGKGYAMVESSEGPLWW
                     QEIDVPAQGLDLTIPVDKTWNRHDLYLSTLVVRLGDKSRSATPKRAVGVLHLPLGDEN
                     RRLDLALETPTKMRPNQPLTVKIKASNKNGEMPKQVNVLVSAVDSGVLNITDYVTPDP
                     WQAFFGQKRYGADIYDIYGQVIEGQGRLAALRFGGDGDELKRGGKPPVNHVNIVAQQA
                     LPVTLNEQGEGSVTLPIGDFNGELRVMAQAWTADDFGSNESKVIVAAPVIAELNMPRF
                     MASGDTSRLTLDITNLTDKPQKLNVALTASGLLELVSNSPAPVELAPGVRTTLFIPVR
                     ALTGYGDGDIQATISGLALPGETVADQHKQWKIGVRPAFPAQTVNYGTALQPGETWAL
                     PADGLQNFSPVTLEGQLLLSGKPPLNIARYIKELKAYPYGCLEQTASGLFPSLYTNAA
                     QLQALGIKGDSDEKRRASVDIGISRLLQMQRDNGGFALWDKNGDEEYWLTAYVMDFLV
                     RAGEQGYSVPTDAINRGNERLLRYLQDPGMISIPYADNLKASKFAVQSYAALVLARQQ
                     KAPLGALREIWEHRADAASGLPLLQLGVALKIMGDATRGEEAIVLALKTPRNSDERIW
                     LGDYGSPLRDSALMLSLLEENKLLPDEQYSLLNTLSQQAFGERWLSTQESNALFLAAR
                     TLQDLPGKWQAQTTFSAEPLTGEKAQTSNLNSDQLATLQVTNSGDQPLWLRVDASGYP
                     QSAPLPASNVLQIERHILGTDGKSKSLDSLRSGDLVLVWLQVKASNSVPDALVVDLLP
                     AGLELENQNLANGGASLEQSGGEVQNLLNQMQQASIKHIEFRDDRFVAAVAVDEYQPV
                     TLVYLARAVTPGTYQVPQPMVESMYVPQWRATGAADDLLIVRP"
     gene            16878..17723
                     /gene="sseA"
                     /locus_tag="NBI74_000659"
     CDS             16878..17723
                     /gene="sseA"
                     /locus_tag="NBI74_000659"
                     /EC_number="2.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-mercaptopyruvate sulfurtransferase"
                     /protein_id="HCD1931035.1"
                     /translation="MSTTWFVGADWLAEHIDDPEIQIIDARMASPGQEDRNVAQEYLN
                     GHIPGAVFFDIEALSDHTSPLPHMLPRPETFAVAMRELGVNQDKHLIVYDEGNLFSAP
                     RAWWMLRTFGVEKVSILGGGLAGWLRDDLLLEEGAVELPEGEFNAAFNPEAVVKVTDV
                     LLASHENTAQIIDARPATRFNAEVDEPRPGLRRGHIPGALNVPWTELVREGELKTTDE
                     LDAIFFGRGVSYDKPIIVSCGSGVTAAVVLLALATLDVPNVKLYDGAWSEWGARADLP
                     VEPVK"
     gene            complement(18216..18992)
                     /gene="sseB"
                     /locus_tag="NBI74_000660"
     CDS             complement(18216..18992)
                     /gene="sseB"
                     /locus_tag="NBI74_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311415.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enhanced serine sensitivity protein SseB"
                     /protein_id="HCD1931036.1"
                     /translation="MSETKNELEDLLEKAATEPAHRPAFFRTLLESTVWVPGTAAQGE
                     AVVEDSALDLQHWEKEDGTSVIPFFTSLEALQQAVEDEQAFVVMPVRTLFEMTLGETL
                     FLNAKLPTGKEFMPREISLLIGEEGNPLSSQEVLEGGESLILSEVAEPPAQMIDSLTT
                     LFKTIKPVKRAFICSIKENEEAQPNLLIGIEADGDIEEIIQVAGSVATDTLPGDEPID
                     ICQVKKGEKGISHFITEHIAPFYERRWGGFLRDFKQNRII"
     gene            complement(19134..20417)
                     /gene="pepB"
                     /locus_tag="NBI74_000661"
     CDS             complement(19134..20417)
                     /gene="pepB"
                     /locus_tag="NBI74_000661"
                     /EC_number="3.4.11.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase PepB"
                     /protein_id="HCD1931037.1"
                     /translation="MTEAMKITLSTQPADVRWGEKATYSINNDGITLHLNGADDLGLI
                     QRAARKVDGLGIKHVQLSGEGWDADRCWAFWQGYKAPKGTRKVEWPELDDAQRQELDN
                     RLMIIDWVRDTINAPAEELGPSQLAQRAVDLICNVAGDRVTYRITKGEDLREQGYMGL
                     HTVGRGSERSPVLLALDYNPTGDKEAPVYACLVGKGITFDSGGYSIKQTAFMDSMKSD
                     MGGAATVTGALAFAITRGLNKRVKLFLCCADNLISGNAFKLGDIITYRNGKKVEVMNT
                     DAEGRLVLADGLIDASAQKPELIIDAATLTGAAKTALGNDYHALFSFDDALAGRLLAS
                     ASQENEPFWRLPLAEFHRSQLPSNFAELNNTGSAAYPAGASTAAGFLSHFVENYQQGW
                     LHIDCSATYRKAPVEQWSAGATGLGVRTIANLLTA"
     gene            complement(20476..20676)
                     /gene="iscX"
                     /locus_tag="NBI74_000662"
     CDS             complement(20476..20676)
                     /gene="iscX"
                     /locus_tag="NBI74_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004104502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HCD1931038.1"
                     /translation="MGLKWTDSREIGEALYDAYPDIDPKTVRFTDMHQWICALEDFDD
                     DPQASNEKILEAILLVWLDEAE"
     gene            complement(20688..21023)
                     /gene="fdx"
                     /locus_tag="NBI74_000663"
     CDS             complement(20688..21023)
                     /gene="fdx"
                     /locus_tag="NBI74_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015683702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISC system 2Fe-2S type ferredoxin"
                     /protein_id="HCD1931039.1"
                     /translation="MPKIVILPHQDLCPDGAVLEANSGETILDVALRNGIEIEHACEK
                     SCACTTCHCIVREGFDSLPESSEQEDDMLDKAWGLEPESRLSCQARVTDEDLVVEIPR
                     YTINHAREH"
     gene            complement(21025..22875)
                     /gene="hscA"
                     /locus_tag="NBI74_000664"
     CDS             complement(21025..22875)
                     /gene="hscA"
                     /locus_tag="NBI74_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly chaperone HscA"
                     /protein_id="HCD1931040.1"
                     /translation="MALLQISEPGLSAAPHQRRLAAGIDLGTTNSLVATVRSGQAETL
                     ADHEGRHLLPSVVHYQQQGHSVGYDARTNAALDTANTISSVKRLMGRSLADIQQRYPH
                     LPYQFQASENGLPMIETAAGLLNPVRVSADILKALAARATEALAGELDGVVITVPAYF
                     DDAQRQGTKDAARLAGLHVLRLLNEPTAAAIAYGLDSGQEGVIAVYDLGGGTFDISIL
                     RLSRGVFEVLATGGDSALGGDDFDHLLADYIREQAGIPDRSDNRVQRELLDAAIAAKI
                     ALSDADSVTVNVAGWQGEISREQFNELIAPLVKRTLLACRRALKDAGVEADEVLEVVM
                     VGGSTRVPLVRERVGEFFGRPPLTSIDPDKVVAIGAAIQADILVGNKPDSEMLLLDVI
                     PLSLGLETMGGLVEKVIPRNTTIPVARAQDFTTFKDGQTAMSIHVMQGERELVQDCRS
                     LARFALRGIPALPAGGAHIRVTFQVDADGLLSVTAMEKSTGVEASIQVKPSYGLTDSE
                     IASMIKDSMSYAEQDVKARMLAEQKVEAARVLESLHGALAADAALLSAAERQVIDDAA
                     AHLSEVAQGDDVDAIEQAIKNVDKQTQDFAARRMDQSVRRALKGHSVDEV"
     gene            complement(22892..23407)
                     /gene="hscB"
                     /locus_tag="NBI74_000665"
     CDS             complement(22892..23407)
                     /gene="hscB"
                     /locus_tag="NBI74_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone HscB"
                     /protein_id="HCD1931041.1"
                     /translation="MDYFTLFGLPARYQLDTQALSLRFQDLQRQYHPDKFASGSQAEQ
                     LAAVQQSATINQAWQTLRHPLMRAEYLLSLHGFDLASEQHTVRDTAFLMEQLELREEL
                     DEIEQAKDEARLESFIKRVKKMFDTRHQLMVEQLDNETWDAAADTVRKLRFLDKLRSS
                     AEQLEEKLLDF"
     gene            complement(23503..23826)
                     /gene="iscA"
                     /locus_tag="NBI74_000666"
     CDS             complement(23503..23826)
                     /gene="iscA"
                     /locus_tag="NBI74_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein IscA"
                     /protein_id="HCD1931042.1"
                     /translation="MSITLSDSAAARVNTFLANRGKGFGLRLGVRTSGCSGMAYVLEF
                     VDEPTPEDIVFEDKGVKVVVDGKSLQFLDGTQLDFVKEGLNEGFKFTNPNVKDECGCG
                     ESFHV"
     gene            complement(23843..24229)
                     /gene="iscU"
                     /locus_tag="NBI74_000667"
     CDS             complement(23843..24229)
                     /gene="iscU"
                     /locus_tag="NBI74_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HCD1931043.1"
                     /translation="MAYSEKVIDHYENPRNVGSFDNNDENVGSGMVGAPACGDVMKLQ
                     IKVNDEGIIEDARFKTYGCGSAIASSSLVTEWVKGKSLDEAQAIKNTDIAEELELPPV
                     KIHCSILAEDAIKAAIADYKSKREAK"
     gene            complement(24257..25471)
                     /gene="iscS"
                     /locus_tag="NBI74_000668"
     CDS             complement(24257..25471)
                     /gene="iscS"
                     /locus_tag="NBI74_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017459011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HCD1931044.1"
                     /translation="MKLPIYLDYSATTPVDPRVAEKMMQFMTMDGTFGNPASRSHRFG
                     WQAEEAVDIARNQIADLVGADPREIVFTSGATESDNLAIKGAANFYQKKGKHIITSKT
                     EHKAVLDTCRQLEREGFEVTYLAPQRNGIIDLKELEAAMRDDTILVSIMHVNNEIGVV
                     QDIAAIGEMCRARGIIYHVDATQSVGKLPIDLSQLKVDLMSFSGHKIYGPKGIGALYV
                     RRKPRVRIEAQMHGGGHERGMRSGTLPVHQIVGMGEAYRIAKEEMATEMERLRGLRNR
                     LWNGIKDIEEVYLNGDLEHGAPNILNVSFNYVEGESLIMALKDLAVSSGSACTSASLE
                     PSYVLRALGLNDELAHSSIRFSLGRFTTEEEIDYTIELVRKSIGRLRDLSPLWEMYKQ
                     GVDLNSIEWAHH"
     gene            complement(25583..26071)
                     /gene="iscR"
                     /locus_tag="NBI74_000669"
     CDS             complement(25583..26071)
                     /gene="iscR"
                     /locus_tag="NBI74_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly transcriptional regulator
                     IscR"
                     /protein_id="HCD1931045.1"
                     /translation="MRLTSKGRYAVTAMLDVALNSEAGPVPLADISERQGISLSYLEQ
                     LFSRLRKNGLVSSVRGPGGGYLLGKDASSIAVGEVISAVDESVDATRCQGKGGCQGGD
                     KCLTHALWRDLSDRLTGFLNNITLGELVNNQEVLDVSGRQHTHDAPRTRTQDAIDVKL
                     RA"
     gene            complement(26342..27082)
                     /gene="trmJ"
                     /locus_tag="NBI74_000670"
     CDS             complement(26342..27082)
                     /gene="trmJ"
                     /locus_tag="NBI74_000670"
                     /EC_number="2.1.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (cytosine(32)/uridine(32)-2'-O)-methyltransferase TrmJ"
                     /protein_id="HCD1931046.1"
                     /translation="MLQNIRIVLVETSHTGNMGSVARAMKTMGLTNLWLVNPLVKPDS
                     QAIALAAGASDVIGNAHIVDTLDEALAGCSLVVGTSARSRTLPWPMLDPRECGLKSVA
                     EAANTPVALVFGRERVGLTNEELQKCHYHVAIAANPEYSSLNLAMAVQVIAYEVRMAW
                     LATQENGEQVEHEETPYPLVDDLERFYGHLEQTLLASGFIRENHPGQVMNKLRRLFTR
                     ARPESQELNILRGILASIEQQNKGNKAE"
     gene            27201..28004
                     /gene="suhB"
                     /locus_tag="NBI74_000671"
     CDS             27201..28004
                     /gene="suhB"
                     /locus_tag="NBI74_000671"
                     /EC_number="3.1.3.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000553460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-1-monophosphatase"
                     /protein_id="HCD1931047.1"
                     /translation="MHPMLNIAVRAARKAGNLIAKNYETPDAVEASQKGSNDFVTNVD
                     KAAEAVIIDTIRKSYPQHTIITEESGELEGTDQDVQWVIDPLDGTTNFIKRLPHFAVS
                     IAVRIKGRTEVAVVYDPMRNELFTATRGQGAQLNGYRLRGSTARDLDGTILATGFPFK
                     AKQYATTYINIVGKLFNECADFRRTGSAALDLAYVAAGRVDGFFEIGLRPWDFAAGEL
                     LVREAGGIVSDFTGGHNYMLTGNIVAGNPRVVKAMLANMRDELSDALKR"
     gene            28149..29003
                     /locus_tag="NBI74_000672"
     CDS             28149..29003
                     /locus_tag="NBI74_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417029.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCD1931048.1"
                     /translation="MALPVNKRVLKILFILFVVAFCVYLVPRVAINFFYYPDDKIYGP
                     DPWSAESVEFTAKDGTRLQGWFIPSSTGPADNAIATIIHAHGNAGNMSAHWPLVSWLP
                     ERNFNVFMFDYRGFGKSKGTPSLAGLLDDTQSAINVVRHRSDVNPQRLVLFGQSIGGA
                     NILDVIGQGDREGIRAVILDSTFASYATIANQMIPGSGYLLDESYSGENYIASVSPIP
                     LLLIHGKADHVIPWQHSEKLYSLAKEPKRLILIPDGEHIDAFSDRHGDVYREQMVDFI
                     LSALNPQN"
     gene            29194..30474
                     /gene="csiE"
                     /locus_tag="NBI74_000673"
     CDS             29194..30474
                     /gene="csiE"
                     /locus_tag="NBI74_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary phase inducible protein CsiE"
                     /protein_id="HCD1931049.1"
                     /translation="MMPTLAPPSVLSAPQRRCQILLTLFQPGLTATTATFSELNGVDD
                     DIASLDISETGREILRYHQLTLTTGYDGSYRVEGTVLNQRLCLFHWLRRGFRLCPSFI
                     TSRFTPALKSELKRRGIARNFYDDTNLQALVNLCSRRLQKRFETRDIHFLCLYLQYCL
                     LQHHAGITPQFNPLQRRWAESCLEFQVAQEIGRHWQRRALQPVPPDEPLFMALLFSML
                     RVPDPLRDAHQRDRQLRQSIKRLVNHFRELGNVRFYDEQGLCDQLYTHLAQALNRSLF
                     AIGIDNTLPEEFARLYPRLVRTTRAALAGFESEYGVHLSDEESGLVAVIFGAWLMQEN
                     NLHEKQIILLTGNDSEREAQIEQQLRELTLLPLNIKHMSVKAFLQTGAPRGAALIIAP
                     YTMPLPLFSPPLIYTDLTLTTHQQEQIRKMLESA"
     gene            complement(30466..31605)
                     /locus_tag="NBI74_000674"
     CDS             complement(30466..31605)
                     /locus_tag="NBI74_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate MFS transporter"
                     /protein_id="HCD1931050.1"
                     /translation="MVLQSTRWLALGYFTYFFSYGIFLPFWSVWLKGIGLTPETIGLL
                     LGAGLVARFLGSLLIAPRVSDPSRLISALRVLALLTLLFAVAFWAGAHVAWLMLVMIG
                     FNLFFSPLVPLTDALANTWQKQFPLDYGKVRLWGSVAFVIGSALTGKLVSMFDYRVIL
                     ALLTLGVASMLLGFLIRPTIQPQGASRQQESTGWSAWLALVRQNWRFLACVCLLQGAH
                     AAYYGFSAIYWQAAGYSASAVGYLWSLGVVAEVIIFALSNKLFRRCSARDMLLISAIC
                     GVVRWGIMGATTALPWLIVVQILHCGTFTVCHLAAMRYIAARQGSEVIRLQAVYSAVA
                     MGGSIAIMTVFAGFLYQYLGHGVFWVMALVALPAMFLRPKVVPSC"
     gene            31975..32397
                     /locus_tag="NBI74_000675"
     CDS             31975..32397
                     /locus_tag="NBI74_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HCD1931051.1"
                     /translation="MNSLRYFDFGTARPVLLLIARIAVVLIFIIFGFPKMMGFDGTVQ
                     YMASLGAPMPMLAAIIAVVMEVPAAILIVLGFFTRPLAVLFIFYTLGTAVIGHHYWDM
                     TGDAVGPNMINFWKNVSIAGAFLLLAITGPGAISLDRR"
     gene            complement(32445..33317)
                     /locus_tag="NBI74_000676"
     CDS             complement(32445..33317)
                     /locus_tag="NBI74_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HCD1931052.1"
                     /translation="MTIYTLSHGPLKLDVSDQGGVIEGFWRDTTPLLRPGKKSGVATD
                     ASCFPLVPFANRVSGNRFVWQGREYQLQPNVEWDAHYLHGDGWLGEWQCVSRSDDSLC
                     LVYEHLSGVYHYRVSQAFHLTADTLTVTLAVTNQGAETLPFGTGWHPYFPLSPQTRIQ
                     AQASGYWLEREQWLAGEFCEQLPQELDFNQLAPLPRQWVNNGFAGWNGKARIEQPQEG
                     YAIIMETTPPAPCYFIFVSDPAFDKGYAFDFFCLEPMSHAPDDHHRPEGGDLIALAPG
                     ESTISEMSLRVALL"
     gene            complement(33329..34423)
                     /locus_tag="NBI74_000677"
     CDS             complement(33329..34423)
                     /locus_tag="NBI74_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HCD1931053.1"
                     /translation="MKINRIVNEGFMKTMLAAYLPGNSTVDLREVAVPTPGINQVLIK
                     MKSSGICGSDVHYIYHQHRATAAAPDKPLYQGFINGHEPCGQIVAMGQGCRHFKEGDR
                     VLVYHISGCGFCPNCRRGFPISCTGEGKAAYGWQRDGGHAEYLLAEEKDLILLPDALS
                     YEDGAFISCGVGTAYEGILRGEVSGSDNVLVVGLGPVGMMAMMLAKGRGAKRIIGVDM
                     LPERLAMAKQLGVMDHGYLATTEGLPQIIAELTHGGADVALDCSGNAAGRLLALQSTA
                     DWGRVVYIGETGKVEFEVSADLMHHQRRIIGSWVTSLFHMEKCAHDLTDWKLWPRNAI
                     THRFSLEQAGDAYALMASGKCGKVVINFPD"
     gene            complement(34456..35454)
                     /locus_tag="NBI74_000678"
     CDS             complement(34456..35454)
                     /locus_tag="NBI74_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCD1931054.1"
                     /translation="MSASSLPLPQGKSVSLKQFVSRHINEIGLLVVIAILYLVFSLNA
                     PGFISLNNQMNVLRDAATIGIAAWAMTLIIISGEIDVSVGPMVAFVSVCLAFLLQFEV
                     PLAIACLLVLLLGALMGTLAGVLRGVFNVPSFVATLGLWSALRGMGLFMTNALPVPIN
                     ENEVLDWLGGQFLGVPVSALIMMVLFALFVFISRKTAFGRSVFAVGGNATAAQLCGIN
                     VRRVRILIFTLSGLLAAVTGILLAARLGSGNAGAANGLEFDVIAAVVVGGTALSGGRG
                     SLFGTLLGVLVITLIGNGLVLLGINSFFQQVVRGVIIVVAVLANILLTQRSSKAKR"
     gene            complement(35457..36986)
                     /locus_tag="NBI74_000679"
     CDS             complement(35457..36986)
                     /locus_tag="NBI74_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HCD1931055.1"
                     /translation="MFTATEAVPVAKVVAGNKRYPGVVALDNVNFTLKKGEVRALLGK
                     NGAGKSTLIRMLTGSERPDSGDIWIGETRLEGDEATLTRRAAELGVRAVYQELSLVEG
                     LTVAENLCLGQWPRRNGMIDYLQMAQDAQRCLQALGVDVSPEQLVSTLSPAQKQLVEI
                     ARVMKGEPRVVILDEPTSSLASAEVELVISAVKKMSALGVAVIYVSHRMEEIRRIASC
                     ATVMRDGQVAGDVMLENTSTHHIVSLMLGRDHVDIAPVAPQEIMDQAVLEVRALRHKP
                     KLEDISFTLRRGEVLGIAGLLGAGRSELLKAIVGLETYEQGEIVINGEKIMRPDYGDM
                     LKRGIGYTPENRKEAGIIPWLGVDENTVLTNRQKISANGVLQWSTIRRLTEEVMQRMT
                     VKAASSETPIGTLSGGNQQKVVIGRWVYAASQILLLDEPTRGVDIEAKQQIYRIVREL
                     AAEGKSVVFISSEVEELPLVCDRILLLQHGTFSQEFHSPVNVDELMSAILSTDEFYRG
                     T"
     gene            complement(37009..37992)
                     /locus_tag="NBI74_000680"
     CDS             complement(37009..37992)
                     /locus_tag="NBI74_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HCD1931056.1"
                     /translation="MPKKMTRTRNLLLMATLLGSALFARASDKEMTIGAIYLDTQGYY
                     AGVRQGVQDAAKDSSVQVQLIETNAQGDISKESTFVDTLVARNVDAIILSAVSENGSS
                     RTVRRASEAGIPVICYNTCINQKGVDKYVSAYLVGDPLEFGKKLGNAAADYFIANKID
                     QPKIAVINCEAFEVCVQRRKGFEEVLKTRVPGAQIVANQEGTVLDKAISVGEKLIIST
                     PDLNAIMGESGGATLGAVKAVRNQNQAGKIAVFGSDMTTEIAQELENNQVLKAVVDIS
                     GKKMGNAVFTQTLKVINKQADGEKVIQVPIDLYTKTEDGKQWLATHVDGLP"
     gene            complement(38089..41370)
                     /locus_tag="NBI74_000681"
     CDS             complement(38089..41370)
                     /locus_tag="NBI74_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5107 domain-containing protein"
                     /protein_id="HCD1931057.1"
                     /translation="MTPVKVWQERVEIPTYETGPQDIHPMFLENRVYQGSSGAVYPYG
                     VTDTLSEQKTLKSWQAVWLENDYIKVMILPELGGRVHRAWDKVKQRDFVYHNEVIKPA
                     LVGLLGPWISGGIEFNWPQHHRPTTFMPVDFTLEAHDDGAQTVWVGETESMHGLQVMT
                     GFTLRPDRAALEIASRVYNGNATPRHFLWWANPAVKGGEGHQSVFPPDVTAVFDHGKR
                     AVSAFPIATGTYYKVDYSAGVDISRYKNVPVPTSYMAEKSQYDFVGAWCHDEDGGLLH
                     VANHHIAPGKKQWSWGHSEFGQAWDKSLTDNNGPYIELMTGIFADNQPDFTWLDAYEE
                     KRFEQYFLPYHSLGMVQNASRDAVIKLQRSERGIEWGLYAISPLNGYRLAIREIGKCN
                     ALLDDAVALTPATAIQGVLHGINPERLTIELSDADGNIVLSYHEHQPQALPLPDVAKA
                     PLAAQDITSTDEAWFIGQHLEQYHHASRSPFDYYLRGVALDPLDYRCNLALAMLEYNR
                     ADFPQAVVYASQALKRAHALNKNPQCGQASLIRASAYERQGQYQQAEEDFWRAVWSGN
                     SKAGGYYGLARLAARNGNFDAGLDFCQQSLRACPTNQEVLCLHNLLLVLSGRQDNARL
                     QREKLLRDYPLNATLWWLNWFDGRSESALAQWRGLCQGRDVNALMTAGQLINWGMPAL
                     AADMLNALDCQRTLPLYLQASLLPKAERGELVAKAIDAFPQFVRFPNTLEEVAALESI
                     EECWFARHLLACFYYNKRSYGKAIALWQRCVEMSPEFADGWRGLAIHAWNKQHDYELA
                     ARYLDNAYQLAPQDARLLFERDLLDKLSGVTPEKRLARLEDNLEIALKRDDMTAELLN
                     LWHLTGQADKAADILATRKFHPWEGGEGKVTSQFILNQLLRAWQHLDDREPQQASELL
                     HAALHYPENLSEGRLPGQTDNDIWFWQAVCANAQGDETEATRCLRLAATGDRTINIHS
                     YYNDQPVDYLFWQGMALRLLGEQQTAQQLFSEMKQWAQEMAKTSIEADFFAVSQPDLL
                     SLYGDLQQQHKEKCLMVAMLAAAGLGEVAHYESARAELMAINPAWPKAALFTTVMPFI
                     FSYVH"
     gene            41488..42681
                     /locus_tag="NBI74_000682"
     CDS             41488..42681
                     /locus_tag="NBI74_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HCD1931058.1"
                     /translation="MRACINNQQIRHHNKCVILELLYRQKRANKSTLARLAQISIPAV
                     SNILQELESEKRVVNIDDESQTRGHSSGTWLIAPEGDWTLCLNVTPTSIECQVANACL
                     SPKGEFEYLQIDAPTPQALLSEIEKCWHRHRKLWPDRTINLALAIHGQVDPVTGVSQT
                     MPQAPWATPIEVKYLLEEKLGIRVMVDNDCVMLALAEKWQNNSQVRDFCVINVDYGIG
                     SSFVINEQIYRGSLYGSGQIGHTIVNPDGVVCDCGRYGCLETVASLSALKKQARVWLK
                     SQPVNTQLNPEKLTTAQLIAAWQSGEPWFTSWVDHSANAIGLSLYNFLNILNINQIWL
                     YGRSCAFGENWLNTIIRQTGFNPFDRDEGPSVKATQIGFGQLSRAQQVLGIGYLYVEA
                     QLRQI"
     gene            complement(42745..43998)
                     /gene="glyA"
                     /locus_tag="NBI74_000683"
     CDS             complement(42745..43998)
                     /gene="glyA"
                     /locus_tag="NBI74_000683"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HCD1931059.1"
                     /translation="MLKREMNIADYDAELWQAMEQEKVRQEEHIELIASENYTSPRVM
                     QAQGSQLTNKYAEGYPGKRYYGGCEYVDIVEQLAIDRAKELFGADYANVQPHSGSQAN
                     FAVYTALLEPGDTVLGMNLAHGGHLTHGSPVNFSGKLYNIVPYGIDATGHIDYADLEK
                     QAKEHKPKMIIGGFSAYSGVVDWAKMREIADSIGAYLFVDMAHVAGLVAAGVYPNPVP
                     HAHVVTTTTHKTLAGPRGGLILAKGGSEELYKKLNSAVFPGGQGGPLMHVIAGKAVAL
                     KEAMEPEFKTYQQQVAKNAKAMVEVFLERGYKVVSGGTDNHLFLVDLVDKNLTGKEAD
                     AALGRANITVNKNSVPNDPKSPFVTSGIRVGTPAITRRGFKEAEAKELAGWMCDVLDS
                     INDEAVIERIKGKVLDICARYPVYA"
     gene            44327..45517
                     /gene="hmpA"
                     /locus_tag="NBI74_000684"
     CDS             44327..45517
                     /gene="hmpA"
                     /locus_tag="NBI74_000684"
                     /EC_number="1.14.12.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NO-inducible flavohemoprotein"
                     /protein_id="HCD1931060.1"
                     /translation="MLDAQTIATVKATIPLLVETGPKLTAHFYDRMFTHNPELKEIFN
                     MSNQRNGDQREALFNAVAAYASNIENLPALLPAVEKIAQKHTSFQIKPEQYNIVGEHL
                     LATLDEMFSPGQEVLDAWGKAYGVLANVFINREAEIYNENASKAGGWEGTRDFRIVAK
                     TPRSALITSFELEPVDGGAVAEYRPGQYLGVWLKPEGFPHQEIRQYSLTRKPDGKGYR
                     IAVKREEGGQVSNWLHNHANVGDVVKLVAPAGDFFMAVADDTPVTLISAGVGQTPMLA
                     MLDTLAKAGHTAQVNWFHAAENGDVHAFADEVKELGQSLPRFTAHTWYRQPSEADRAK
                     GQFDSEGLMDLSKLEGAFSDPTMQFYLCGPVGFMQFAAKQLVDLGVKQENIHYECFGP
                     HKVL"
     gene            complement(45562..45900)
                     /gene="glnB"
                     /locus_tag="NBI74_000685"
     CDS             complement(45562..45900)
                     /gene="glnB"
                     /locus_tag="NBI74_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017373484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulatory protein P-II"
                     /protein_id="HCD1931061.1"
                     /translation="MKKIDAIIKPFKLDDVREALAEVGITGMTVTEVKGFGRQKGHTE
                     LYRGAEYMVDFLPKVKIEIVVPDDIVDTCVDTIIRTAQTGKIGDGKIFVFDVARVIRI
                     RTGEEDDAAI"
     gene            complement(45961..47295)
                     /gene="glrR"
                     /locus_tag="NBI74_000686"
     CDS             complement(45961..47295)
                     /gene="glrR"
                     /locus_tag="NBI74_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator GlrR"
                     /protein_id="HCD1931062.1"
                     /translation="MSHKPAHLLLVDDDPGLLKLLGLRLTSEGYSVVTAESGAEGLRV
                     LNREKVDLVISDLRMDEMDGMQLFAEIQKVQPGMPVIILTAHGSIPDAVAATQQGVFS
                     FLTKPVDKDALYQAIDDALEQSAPATDERWREAIVTRSPLMLRLLEQARLVAQSDVSV
                     LINGQSGTGKEIFAQAIHNASPRNSKPFIAINCGALPEQLLESELFGHARGAFTGAVS
                     NREGLFQAAEGGTLFLDEIGDMPAPLQVKLLRVLQERKVRPLGSNRDIDIDVRIISAT
                     HRDLPKAMARGEFREDLYYRLNVVSLKIPALAERTEDIPLLANHLLRQAAERHKPFVR
                     AFSTDAMKRLMTASWPGNVRQLVNVIEQCVALTSSPVISDALVEQALEGENTALPTFV
                     EARNQFELNYLRKLLQITKGNVTHAARMAGRNRTEFYKLLSRHELDANDFKE"
     gene            complement(47285..47998)
                     /gene="qseG"
                     /locus_tag="NBI74_000687"
     CDS             complement(47285..47998)
                     /gene="qseG"
                     /locus_tag="NBI74_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system QseEF-associated
                     lipoprotein QseG"
                     /protein_id="HCD1931063.1"
                     /translation="MRHIFQRLLPRRLWLAGLPCLALLGCVQSHNRPAIDTPAEEKIP
                     VYQLADYLSTECSDIWALQGKSTETNPLYWLRAMDCADRLMPAQSRQQARQYDDGNWQ
                     NTFKQGILLADAKITPYERRQLVARIDALSTEIPAQVRPLYQLWRDGQALQLQLAEER
                     QRYSKLQQSSDSELDTLRQQHHVLQQQLELTTRKLENLTDIERQLSTRKPAGNFSPDT
                     PHESEKPAPSTDEVTPDEP"
     gene            complement(48163..49590)
                     /gene="qseE"
                     /locus_tag="NBI74_000688"
     CDS             complement(48163..49590)
                     /gene="qseE"
                     /locus_tag="NBI74_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two component system sensor histidine kinase
                     QseE/GlrK"
                     /protein_id="HCD1931064.1"
                     /translation="MKRWPVFPRSLRQLVMLAFLLILLPLLVLAWQAWQSLNALSDQA
                     ALVNRTTLIDARRSEAMTNAALEMERSYRQYCVLDDPTLAKVYQSQRKRYSEMLDAHA
                     GVLPDDKLYQALRQDLNNLAQLQCNNSGPDAAAAARLEAFASANTEMVQATRTVVFSR
                     GQQLQREIAERGQYFGWQSLVLFLVSLVMVLLFTRMIIGPVKNIERMINRLGEGRSLG
                     NSVSFSGPSELRSVGQRILWLSERLSWLESQRHQFLRHLSHELKTPLASMREGTELLA
                     DQVVGPLTPEQKEVVSILDSSSRNLQKLIEQLLDYNRKQADSAVELENVELAPLVETL
                     VSAHSLPARAKMMHTDVDLKATACLAEPMLLMSVLDNLYSNAVHYGAESGNICLRSSL
                     HGARVYIDVINTGTPIPQEERAMIFEPFFQGSHQRKGAVKGSGLGLSIARDCIRRMQG
                     ELYLVDESGQDVCFRIELPSSKNTK"
     gene            complement(50166..54053)
                     /gene="purL"
                     /locus_tag="NBI74_000689"
                     /gene_synonym="purI"
     CDS             complement(50166..54053)
                     /gene="purL"
                     /locus_tag="NBI74_000689"
                     /gene_synonym="purI"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HCD1931065.1"
                     /translation="MMEILRGSPALSAFRINKLLARFQAARLPVHTIYAEYVHFADLN
                     APLNDDEHAQLERLLKYAPALASHAPQGKLLLVTPRPGTISPWSSKATDIAHNCGLQQ
                     VNRLERGMAYYIEAGTLTNEQWQQVTAELHDRMMETVFFALDDAEQLFAHHQPTPVTS
                     VDLLGQGRQALIDANLRLGLALAEDEIDYLQDAFTKLGRNPNDIELYMFAQANSEHCR
                     HKIFNADWIIDGEQQPKSLFKMIKNTFETTPDHVLSAYKDNAAVMEGSEVGRYFADHE
                     TGRYDFHQEPAHILMKVETHNHPTAISPWPGAATGSGGEIRDEGATGRGAKPKAGLVG
                     FSVSNLRIPGFEQPWEEDFGKPERIVTALDIMTEGPLGGAAFNNEFGRPALNGYFRTY
                     EEKVNSHNGEELRGYHKPIMLAGGIGNIRADHVQKDEINVGAKLVVLGGPAMNIGLGG
                     GAASSMASGQSDADLDFASVQRDNPEMERRCQEVIDRCWQLGDANPILFIHDVGAGGL
                     SNAMPELVSDGGRGGKFELRDILSDEPGMSPLEIWCNESQERYVLAVAADQLPLFDEL
                     CKRERAPYAVIGEATEELHLSLHDRHFDNQPIDLPLDVLLGKTPKMTRDVQTLKAKGD
                     ALAREGITIADAVKRVLHLPTVAEKTFLVTIGDRSVTGMVARDQMVGPWQVPVANCAV
                     TTASLDSYYGEAMAIGERAPVALLDFAASARLAVGEALTNIAATQIGDIKRIKLSANW
                     MAAAGHPGEDAGLYEAVKAVGEELCPALGLTIPVGKDSMSMKTRWQEGNEEREMTSPL
                     SLVISAFARVEDVRHTITPQLSTEDNALLLIDLGKGNNALGATALAQVYRQLGDKPAD
                     VRDVAQLKGFYDAIQALVAQRKLLAYHDRSDGGLLVTLAEMAFAGHCGIDADIASLGD
                     DRLAALFNEELGAVIQVRAADREAVEAVLAQHGLADCVHYVGQAVSGDRFVITANGQT
                     VFSESRTTLRVWWAETTWQMQRLRDNPECADQEHQAKSNDADPGLNVKLSFDINEDVA
                     APFIATGVRPKVAVLREQGVNSHVEMAAAFHRAGFDAIDVHMSDLLAGRTGLEGFHAL
                     VACGGFSYGDVLGAGEGWAKSILFNDRVRDEFATFFHRPQTLALGVCNGCQMMSNLRE
                     LIPGSELWPRFVRNTSDRFEARFSLVEVTQSPSLLLQGMVGSQMPIAVSHGEGRVEVR
                     DAAHLAALESKGLVALRYVDNFGKVTETYPANPNGSPNGITAVTTESGRVTIMMPHPE
                     RVFRTVSNSWHPENWGEDGPWMRIFRNARKQLG"
     gene            54311..55867
                     /gene="mltF"
                     /locus_tag="NBI74_000690"
     CDS             54311..55867
                     /gene="mltF"
                     /locus_tag="NBI74_000690"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltF"
                     /protein_id="HCD1931066.1"
                     /translation="MKKLKINYLFIGILALLLAVALWPSIPWFGKADNRIAAIQARGE
                     LRVSTIHTPLTYNEINGKPFGLDYELAKQFADYLGVKLKVTVRQNISQLFDDLDNGNA
                     DLLAAGLVYNSERVKNYQPGPTYYSVSQQLVYKVGQYRPRTLGNLTAEQLTVAPGHVV
                     VNDLQTLKDTKFPELSWKVDDKKGSAELMEDVIEGKLDYTIADSVAISLFQRVHPELA
                     VALDITDEQPVTWFSPLDGDNTLSAALLDFFNEMNEDGTLARIEEKYLGHGDDFDYVD
                     TRTFLRAVDAVLPQLKPLFEKYAEEIDWRLLAAIAYQESHWDAQATSPTGVRGMMMLT
                     KNTAQSLGITDRTDAEQSISGGVRYLQDMMSKVPESVPENERIWFALAAYNMGYAHML
                     DARALTAKTKGNPDSWADVKQRLPLLSQKPYYSKLTYGYARGHEAYAYVENIRKYQIS
                     LVGYLQEKEKQATEAAMQLAQDYPAVSPTELGKEKFPFLSFLSQSSSNYLTHSPSLLF
                     SRKGSEEKQN"
     gene            complement(55864..56400)
                     /gene="tadA"
                     /locus_tag="NBI74_000691"
     CDS             complement(55864..56400)
                     /gene="tadA"
                     /locus_tag="NBI74_000691"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HCD1931067.1"
                     /translation="MRRAFITGVFFLSEVEFSYEYWMRHAMTLAKRAWDEREVPVGAV
                     LVHNNRVIGEGWNRPIGRHDPTAHAEIMALRQGGLVMQNYRLIDATLYVTLEPCVMCA
                     GAMIHSRIGRVVFGARDAKTGAAGSLMDVLHHPGMNHRVEITEGILADECAALLSDFF
                     RMRRQEIKAQKKAQSSTD"
     gene            complement(56425..57060)
                     /gene="yfhb"
                     /locus_tag="NBI74_000692"
     CDS             complement(56425..57060)
                     /gene="yfhb"
                     /locus_tag="NBI74_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311453.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase C"
                     /protein_id="HCD1931068.1"
                     /translation="MATHERRVVFFDLDGTLHQQDMFGSFLRYLLRRQPLNALLVLPL
                     LPIIAIALLIKGRAARWPMSLLLWGCTFGHNEARLQALQADFVRWFRDNVTAFPLVQE
                     RLTTYLLSSDADIWLITGSPQPLVEAVYFDTPWLPRVNLIASQIQRGYGGWVLTMRCL
                     GHEKVAQLERKIGTPLRLYSGYSDSNQDNPLLYFCQHRWRVTPRGELQQLE"
     gene            57269..58117
                     /locus_tag="NBI74_000693"
     CDS             57269..58117
                     /locus_tag="NBI74_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HCD1931069.1"
                     /translation="MNGLLRIRQRYQGLAQSDKKLADYLLLQPDTARHLSSQQLANEA
                     GVSQSSVVKFAQKLGYKGFPALKLALSEALASQPESPSVPIHNQIRGDDPLRLVGEKL
                     IKENTAAMYATLNVNSEEKLHECVAMLRSARRIILTGIGASGLVAQNFAWKLMKIGFN
                     AAAVRDMHALLATVQASSPDDLLLAISYTGVRRELNLAADEMLRVGGKVLAITGFTPN
                     ALQQRASHCLYTIAEEQATNSASISACHAQGMLTDLLFIALIQQDLELAPERIRHSEA
                     LVKKLV"
     gene            58173..>58396
                     /locus_tag="NBI74_000694"
     CDS             58173..>58396
                     /locus_tag="NBI74_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhL family 4Fe-4S dicluster ferredoxin"
                     /protein_id="HCD1931070.1"
                     /translation="MALLITKKCINCDMCEPECPNEAISMGEHIYEINSDKCTECVGH
                     YETPTCQKVCPLPNTIVKDPQHVETEEQLW"
BASE COUNT        13798 a        16288 c        14551 g        13759 t
ORIGIN      
        1 caataacaaa ttcaacgcgt tttttagata ttaaaccgtg cccatgttgg cagaaataat
       61 ccacagcacc acaagctttt aacacttgta acggctgatg acactcaggg caaagtgcct
      121 ggacggtaaa gtctttgtcg caagttgcac agtgagcagt ctcgccattg cgttcgagag
      181 gggttttgca ggttggacag ttgagtgcca taaatactcc tcgcgtgtta ttcggtttcc
      241 ctctccgtgt ggaaagggaa accagataag tgattactta cttttcttga tgtgcttcat
      301 cagacgctta cgcttgcgca tctgagtcgg cgtcagggta ttacgcttgt tcgcatacgg
      361 gttttcccct tctttgaact gaatacgaat cggcgatccc attacgtcca gggatttgcg
      421 gaagtagttc atcaggtaac gcttgtagga atcaggcagg tctttcacct gattgccgtg
      481 aatcaccaca atcggcgggt tataaccccc ggcgtgggcg tatttcagct tcacacgacg
      541 accgcgtacc agcggcggtt ggtgatcttc aacagccatc gtcatgatgc gcgtcagcat
      601 agaggtcccc acacgacggg tggagctgtc atacgcttca cgtactgatt caaacaagtt
      661 tccaacacca ctgccgtgca aggcagagat aaagtgcaca cgagcaaaat cgataaagcc
      721 cagacggaag tccagcgtct ctttcacctg ctctttcact tcctggctca ggccgtccca
      781 cttgttcact acaatcacaa gtgagcgccc actattgaga ataaagccca gcaaggagag
      841 atcctgatcg gaaatacctt cgcgcgcatc aatcaccaac atcaccacgt tggcatcttc
      901 aatggcctgc aacgttttga ttaccgagaa tttctctacc gcatcggtga ttttgccgcg
      961 tttacgtacg ccagcggtgt caatgagcac atactcacgt ccgtcgcgtt ccattggaat
     1021 gtagatactg tcacgcgtcg tgccaggcat gtcgtaaaca acaacgcgct cttcaccaag
     1081 aatacggtta gtgagtgtag acttacctac gttcgggcga cccacaatcg ccagtttgat
     1141 cggcagactt tgcgggtcga agtcgtcttc ctcttcttct tcgccgttct cttccgcttc
     1201 aaattgcgcc cagtattcgg cgtcttcgtc gacttcttct tgcggtgcga gatcttccat
     1261 ccacggcagc agcacgtgct ccagcagact taatacgcca cgaccgtgag acgcggcgat
     1321 cgggtagatt tcacctaaac caagcgcgta gaaatcaacc actgcctgat cgggatccag
     1381 accgtcagtt ttgtttgcca ccaggaaggt cggtttttca cgggagcgca gatgtttagc
     1441 aatcgcttca tccgccggca tcaggcccgc gcgcgcatcc accataaaca gtacgacgtc
     1501 cgcttcttca atcgccagca gcgactgttc cgccatgcgg gtttctacac cgtcttctgt
     1561 gccatcaatc ccgccggtat caatgcagat aaactcacgg ccttcaattt ccgcacgacc
     1621 gtacttacgg tcacgagtca gacccgggaa atccgcaacc agcgcatctc gggtgcgagt
     1681 tagacggtta aataacgtgg attttcctac gttagggcgc ccgacaagcg cgaccacagg
     1741 taccatgttt aaagcctcat ttttataaat catcagacaa cgcacgctat attcgtgtcg
     1801 ttgttaaaaa caggaaaacg gcccctgtcc aggagccgtt ttcaaagtga acgacagaga
     1861 cgattaacgt gtaatagagt acacggttcc gtcttttgcc tggatcagca gtttgccgtc
     1921 agcagcaacc ggttcagtct ggaaaccgga actatcaact ttttgctggg caacgaaacg
     1981 accatcttcg acgttaatcc agtgcagata accttcactg tcgcctacca ccaggttgcc
     2041 attatacagc accggagaag tcagcaggcg atgcagcaga tcgctttgtg tccacagcgt
     2101 aacgccgcca tcaatggtca acgccatcac ccggtcattt tgatcgacca gatagatgcg
     2161 attgccgtcg acgatgaaat cattcaccga acccagttcg cgtttccaca taatctgacc
     2221 actgcgcaga tcaagcgccg tcaggttacc attataggcc agcgcgaaaa caacgccgtt
     2281 aacaacgacg ggagtcgtgt caacatcgct cagacggtca atttcggtag aaccggtcgc
     2341 ctgggaaata cgctgctgcc aaatcatctg gccctgttcc atcagcactg cgctgacgcg
     2401 accattatcg ccacccacga cggccgcacc aaaagccgtt gccggcgcag actcgccacg
     2461 caaagagagc gaaggcatat cgaggttaac tgtccatttg acagcgccgt cagcttcgtt
     2521 cagcgcttgt aactgaccgt tactggtgtg gattaacacc agaccgtcgc tgaccaccgg
     2581 gcgcgaaagt gcttcacccg cgactttagt ttgccatgcc acagtaccat cgctggtatt
     2641 cagcgcgtaa acctgcgcct tttcggtgcc aatgtagaca tgcccaccag acacggtcac
     2701 gccgccagaa agtaatgcag gatctttaga gaaccagcca tctttctcgg ccaggttgac
     2761 agaccagatt tctttgccgt catccgcatt cagcgctttt actaaaccag cgcggtccgc
     2821 tgcatagaca acgttgtccg ccagtgccgg atgaagattg gaatagaagt tgccaatgcc
     2881 gctaccaacg gaagtgctcc acacagtagt cggcgtaaac tggttttcaa cggttggcaa
     2941 tggggacatc tttaccacat cttcttcgct gttaaacagc gaacagccgc ttaaaagggt
     3001 aacggaaagc agtcctggca gcagtaattt acgcaattgc atcgggtccc tctcagatgg
     3061 acaaattatt aattttcatc tgcatcattt cgctcagtgc cggggtaaca tcgcttttca
     3121 cgcctgcttc ccatgcacta cgcgcacctt gcttatcacc tttgctcagc aatgcttcac
     3181 cacgcaggtc ggcaacaatg gcagcccacc cttcaccttt gatagcatca aggtttttta
     3241 gcgcggcatc agcctgcttg agctgtacct gaacgcgagc aagacgcaga tttatcacgg
     3301 ctttgagatt ttcatcgctc gtgtctgcca gcccctgttg taactgggcg gcagctttct
     3361 ctagttcatt tttgtcaaca aattgctgcg caagttccaa agaagccagc gcaccataag
     3421 tatttttatt ttcagcagca aatttttccg ccgctgggat gctatccggt ttgccttcgc
     3481 tcactgcggt aaccgcattt tgataggcaa gagaagcgga gcgtgcagaa tcaacctgat
     3541 ggctattcca gtagcgccag ccaatcagtg cgccaacgcc caaaatcacc ccaacagcca
     3601 gtgctttgcc attttcagca aaaaagcgtt taaccgcttc aacctggtcg ttttcgttct
     3661 cgtaaatttc cacgctgtcc ttctccttcc ttaacccagt aacgtgcgca aatgcgcggc
     3721 tacgctatcc tgcgcaactg cagtttgctc accagagcgc aaatccttca ctactgctgt
     3781 gccgttagcc acttcagact cacccagcac cacagcaacg cgggcacccc atttatcagc
     3841 acgggcaaac tgtttcttaa agttgccgcc gccgtggttg gtcatcagtt tcacacccgg
     3901 taattcatca cgcagacgct ctgctaatgc catagccgca gattgtgtgt cagcacctga
     3961 agccaccagg tatatatcga caacaggatc ggctttaaat tccggattaa cggcctgaac
     4021 taacaataca agacgttcga ggcccatcgc aaaaccgact gccggtgttg cacgaccacc
     4081 cagttgttcc accagaccgt cataacgacc acctgcacac acggtgccct gggaaccgag
     4141 gctgttagtc acccactcga aaacggtacg gttatagtaa tccagaccgc gcaccagacg
     4201 ctggtttaca gtgtaagcga tccccgcgct ctcaagcagt ttgcacagac cggcaaagtg
     4261 ctcacgagat tcctcgtcca gataatcgcc taatgccgga gcgtcgttga gaagcacctg
     4321 cacttccgga ttttttgaat ccagtacgcg caacgggtta gtgtacatgc ggcgtttgca
     4381 gtcttcgtcc agcttttctt tatgctgctc aaggaatgcc accagcgcat cgcggtaatt
     4441 cgcacgagct tccagcgaac cgatagagtt cagctcaaga gttacgtgct cggaaatacc
     4501 cagcgcgcgc caccagcggg cagtgagcat aatcagttcg gcgtcgatat ccggaccttg
     4561 cagaccgaaa acttcgcagc ccaactgatg gaactgacga taacgccctt tctgcggacg
     4621 ctcgtgacgg aacataggcc cgatatacca cagacgctgt tcctgattgt acagaagacc
     4681 atgctcgatg ccggcgcgta cacagcccgc cgtcccttca gggcgcagag tcaggctgtc
     4741 gccattgcga tcctcaaagg tgtacatctc tttttcaacc acgtcggtga cttcaccaat
     4801 cgcacgtttg aatagcgggg tctgctctac aatcggcaag cggatttcac tgtaaccgta
     4861 gctgccgagc acgtttttca gtgtgccttc aatgcgctgc cagatggccg tttcgccagg
     4921 caggtaatcg ttcatgccgc gaatggcttg aatgtttttt gccacgttta ttctctttct
     4981 gaatataaaa atgaaccctc aacgcttccc tcaatgtttc gggagccatg cgggttcaat
     5041 catacacggg aagcgaggcg cttcccatca cgttattatt tttcaacctg ctgaacgtca
     5101 attcgacgcg cttcgtccag ctgactggct ttcgcacgaa tgcgcgcttc cagctgatcg
     5161 atcatatcgt tgttgtccag acggtctttg cgcacgccat cttcatagag gccgcttttc
     5221 ttgttgccgc cagtgacgcc gagtgtagaa accagcgcct cacctgggcc attcaccacg
     5281 cagccgataa tcgaaacgtc catcggagtg attatatctt ccaggcgttg ctccagcgcg
     5341 ttaaccgtac cgataacatc aaattcctga cgcgaacagg tcgggcaggc gatgaagttg
     5401 atcccgcgcg aacggatacg cagcgatttc aaaatatcga aaccgacttt gatctcttcg
     5461 accggatcgg ccgccagcga tacgcgcagc gtgtcgccga tgccttcaga cagcagcaga
     5521 cctaaaccaa tagcggattt tactgccccg ctgcgcgcac caccggcttc ggtgatcccc
     5581 agatgcagcg gctgatcgat ttgttttgcc agcaaacgat aagactcaac agcgaggaag
     5641 acgtcagacg ctttcacact gactttgaac tgatcgaagt tcaggcgatc gagatgatca
     5701 acatgacgca tggcggattc cagcaacgcc tgcggcgtcg gttcgccata cttttcttgc
     5761 agatcttttt ccaacgatcc ggcgttaacg ccaatacgga tcggaatgtt tttatcgcgc
     5821 gcacagtcaa ccaccatgcg aatacgctct tcattaccga tattgccagg gttaatacgc
     5881 agacaatcga cgccgtattc cgctactttc agcgcaatgc gatagtcgaa gtggatgtca
     5941 gccaccagcg gcacgttaac ctgctgtttg atgagtttga acgcttctgc cgcgtccatc
     6001 gtcggtacgg agacacggac gatatcagcg ccaacgcgtt ccagcgcttt gatttgattg
     6061 accgttgctt cgacgtctgt cgtacgcgta ttggtcatgg actgtacggc gatgggagca
     6121 ccatcgccaa tcggcacatt cccaacgtaa atacgtgttg attttctacg ttgaattgga
     6181 gcctggttat gcatgaaaaa tctcccgcgt tacccgtctg ttactgcgcc ggtgattgtt
     6241 cggcattgag ggtcagacgc gcaacctggt tagttctgat aaaacgactc agatcgacag
     6301 gtttcccttg atactggatc tgtactgcgg ctggcgcacc aattttcagt ttgtacggtg
     6361 cctggccggt taagttcaaa ttaccgtctt tacgctgcat accgctaaac agttttttac
     6421 cggtagcatc ggtgacttcc agccagcaat cggaagtaaa gttcatcacc agcgcattcg
     6481 ggtcaaccgc cggcgtggtc acgccagcct gatcggttgg caacggcgca gcgccatctg
     6541 gtgttgttgt tgccgctggt gccggagtcg cagcggtatc aacatttgcc tgcgaaggtg
     6601 gaacaaccgc attctgttgc ggatcaacag ctggtgctgg cgcagttacg gcaggtgttt
     6661 gcgtgttggt tgcggtggta tccacaggtg atgtcggcgt ggtcgccata gcctgatctg
     6721 tggttgtcga cgtatctaac ggtacactct ggctttgatt attattcagt tccgcagaag
     6781 attgatcggc catagtggtg atctcttcct gctgagcttt gtggtcttgc caccaccagg
     6841 caccgctcag accaataacc acaaacaaca ccagccaggt gaaggtcatc agccagccgt
     6901 cacgtttttt gcggcgttta ccgagggaaa aactctgcat cggcgcaact tttgcagccc
     6961 gtagtggagc ctgcttttcc agccctggca gcagttcttc ttctggaata tgtaccagac
     7021 gcgcataaga gcggatatat ccgcgcagga atgttgaagc aagatcggcg ggtgccttat
     7081 cttcttcaat gtcgcgtacc gtggaaacct tcaggcaaag tcgctcggca acggcctgtt
     7141 gactaagtcc tagttgttcg cgagcattac gcaggcgagc gccggtagta agtgcttcat
     7201 tttggtcgtg cgtggcttca gtattcattc gctgcaggta cgtttaaatg agaattgaga
     7261 tgccggtgaa ccatcatgct cacccacacc gcgaaacatc cgttaagtta accgttatca
     7321 tacagtataa gaccgtctgt gtgctcgtga caaagcccac attcgctaag gctaacttac
     7381 tgttgcatcg ttacatactg ccttaaagtc agcaaaaacg caccgttaat attgaccaga
     7441 caattgcaac ttaatacttc attcataaaa gattacgcca cggtacataa agtaaccgtg
     7501 gcgtaatggc tatcagaccg ctttaatgtc gatggcttca ccctgcatcc gtttacgcag
     7561 ggtacgtttc gtacggtcga taacatcgcc cgccaactga ccacaggcag catcaatgtc
     7621 atcaccacga gttttacgca caatagtggt gaaaccgtag ctcatcagca cttttgagaa
     7681 acggtcgata cggctgttcg aactgcgtcc atacggcgcg cccgggaacg ggttccacgg
     7741 gatcaggttg atcttacacg gcgtatcttt cagcagttct gccagttggt gcgcgtgttc
     7801 agtgccgtcg ttaacgtggt caagcatcac gtattcaata gtgactcggc cctgattggc
     7861 gttggatttc tccagataac ggcgcaccgc cgcaaggaac gtttcgatat tgtacttttt
     7921 gttgatcggc acaatttcgt cacgaatttc gtcgttcggc gcgtgcaggg aaattgccag
     7981 cgcaacgtcg atcatatcgc cgagtttatc cagcgccgga accacaccgg aagtggaaag
     8041 cgtgacgcga cgttttgaca ggccaaaacc gaaatcatca agcatgattt ccatcgccgg
     8101 aacgacgttg ttcaggttga gcagcggctc gcccataccc atcatcacta cgttagtgat
     8161 tgggcgctga ccggtgactt ttgctgcgcc gacaattttc gccgcacgcc acacctggcc
     8221 gataatttcc gatacccgca ggttgcgatt aaagccctgc tgggcggtgg aacagaattt
     8281 acactccagc gcacacccca cctgcgaaga gacgcagagc gtggcacggt cgtcttccgg
     8341 gatatacacc gtttcaacgc gctgatcgcc aacggcgatc gcccatttaa tggtgccgtc
     8401 agatgaacgc tgttcttcaa ccacttccgg tgcacggatt tccgccacct ctttcagttt
     8461 gccgcgcaac actttattga tgtcggtcat ctcatcaaag ttgtcgcagc aatagtgata
     8521 catccacttc atcacctgat cggcgcggaa gggtttttca cctaaatctt taaaaaactc
     8581 ccgcatctgc tgacggttga gatccagcag gttgattttt ccatctttcg tggtgacgtt
     8641 ttcaggtgtg actaattgtt cagacatatg ctattccggc ctcgttatta cacgttatgg
     8701 cccctggagg gttgaaaaaa gaaacgcccc ggtgagcggc tgctcgtccg ggggcgctgc
     8761 attgtacaaa ttctggcgca cggatgccac gtttgcacgc ggcatttacg aaattattaa
     8821 cgggtgcgcg ggcacacttc gccttcgccg aagaaataag cgatttcgcg agcggcagat
     8881 tcgacggaat cagaaccgtg ggtaccattt tcggtcaggc tgtcagcgta atcagcacgc
     8941 agagtaccgg ccagtgcgtt tgccggattg gtcgcgccca gcagatcgcg gtgacgctga
     9001 acagcatttt caccttccag cacggaaaca acgatcgggc cagaagtcat gaattcaacc
     9061 agaccatcaa agaacggttt tccatcgtgt tcagcataaa agccacgagc ctgttcaacg
     9121 gtcaggtgca gcattttggt gccgacaatt ttgaaccctg cagcttcaaa gcgcgcaaag
     9181 atactaccaa tcacgttttt tgctactgcg ttcggtttga tgatggaaaa agtacgttca
     9241 atagccattt ttacctctgt aaattgttct gttgttgtct gtaccagcgt acgagatggc
     9301 gcggattata atgagcaaca gggccgttga ctattgatga aggtaacatt tttttaaaat
     9361 aaacccagtt taagcaacaa tcagggacaa aacagactat tgcatgacaa atttcactgt
     9421 cgctatttgc cccacgtcat ccatcaccag caattgataa tcgcctttat ccgtcaaatg
     9481 cagggtcacg ttgcgcccac gttcagttaa cggttcgccg ttcagaaacc accagcgttc
     9541 acctgcccct ccacttgatt gcaacggcaa agtcgcttcc gctgcgcccg gtaaacgttt
     9601 aataatcgcg ccatcgcgca cacctgttag ctgcagcggg agttgtgcat cgtgaccgta
     9661 tggcgggcaa attgtcgagg ccggaggcaa gcgcacagcg cgacgttctg atgcgggcag
     9721 ccagggttcc agcggtagcg gccagacatt tatcatttct tgtcgcgcct gagggcaatc
     9781 agcggcaaca cgtttgccgt tttcatccag ccagatgggg aaacgaatgc cattaatgcc
     9841 ttcctgctcc ggcagtaata gagttggcgg ctggctcccg tccagcagcc aggttgccag
     9901 gcggcggcga cagttaccgt caccttccgg caaagactgt ccgcccggcc agcagataac
     9961 gccacgactt acagagtcag gacgtgggtc ttccggcaaa ttcacactgc gcgacagtaa
    10021 gatattatta acctgattca acagcggtac ggcactggca aagccaaact gaccaacaac
    10081 gggcgtgccg tccggtctgc cagtccagat cccaatgaca tagcgagcgt taaccccaat
    10141 cgcccaggca tcacgatagc catagctggt gcccgttttc catgccagtg gggcgacgcg
    10201 cggcaaggca ccatccggca agggttgcgc ttcatcagcc ataatccggc gaatgatcca
    10261 cgcagccccc gacgacatta aaggccgttc aagcagcgga tcgtcaggct gtaagcgcaa
    10321 tttgcctgcc ttgccgtggc gagcaaacgc ggtatacgct gctgccatat cttccagttt
    10381 tgcgccagcg ccgccgagaa tgagcgaaag attcggcgca gcaccgttgg gcagatacaa
    10441 cggcaatcca acattgcgta actttgccgc aaaccgtttc ggtccatagg cttccagcac
    10501 ctgcacagca ggtaagttca gcgatcgcac cagcgcctca ctcatgctga tcgggccatg
    10561 aaaaccgctg tcaaagttgc caggtcggta atcaccggtg cgtcggggga cgtcttgcaa
    10621 cagcgatgcc gggtggatca cgccttcatc cagcgccaga ccataagcaa acggtttgag
    10681 caccgatcct ggcgatcgga tcgcattgac catatcaaca tgaccaaagc gtgaatcatc
    10741 gttgagatca accgatccca cccagccgcg aacacgcata tcggtatgat caaccacgat
    10801 catcgccagt gagctgcgcg gtggcaatcg tcctttccag ttttgcgcca gttcttccag
    10861 acgtcgttga agaccggcat ccagcgtagt aacgattttg tcgcttttgc ttttaccgag
    10921 catcatgcgc gaaaacagcg gtgccagttg cggcatttgt cggggtgcca gccagatggg
    10981 ttcttccctt gactctttta cccgctcacg ggaccacaca ccttgcacgg ccatccgttc
    11041 gagcacttta ttacgcgcgg cttcggcacg ctccggccaa cgatccgggc gaagacggct
    11101 gggcgcttgc ggcaacaccg ccagcattgc cgcctcggaa tagcttaaat tcgcaggcga
    11161 tttcccgaga taagcccaac ttgccgcacc aatcccctgc aacgtaccgc caaacggggc
    11221 acggttaaga tacaaggtca gaatttcacg tttagacaga tgccattcca gttgcaacgc
    11281 gcgccagagc tgacgaattt tgccgccaaa cgttttgggg tgaggatcaa gcaggcgcgc
    11341 cacctgcatg gtgagcgtgc tgccgccgga aatgacccga cccgacgtga gatcttgcca
    11401 ggctgcacgc gccaccgaga acggattcac ccccggatgc ttccagaacc agcgatcttc
    11461 ataattgatc agtgcttcaa ggtaacgtgg agaaacatct tcgattgtta ccggataacg
    11521 ccagatgccg tcagcatcgg cgaaacgcca gagtggcgta ccatcctgcg ccaccaccac
    11581 gcgtgcagga ttcacttctt gcaatggcag aggccagagt ttatctgctg cccatgctgc
    11641 cagaatgatg ataaaaggcg cggctgtcag cattatccag cagccgcgtt tggttaacag
    11701 gcgaggcatt tacggtctga ctatcaacag atcgtcagcc gcaccagtcg cccgccattg
    11761 aggaacatac attgattcca ccataggctg cggcacctga tacgttcccg gcgtcaccgc
    11821 ccgcgccaga tacaccagcg ttaccggttg atattcatca acggcaaccg ccgccacaaa
    11881 gcgatcgtca cggaactcta tgtgcttaat gctcgcctgc tgcatctggt tcagcaagtt
    11941 ctgcacttca ccaccacttt gctccaggct ggcgccgccg ttcgccagat tctggttttc
    12001 cagttccagc cccgcaggca acagatccac gactaacgca tccggtacac tgttactggc
    12061 tttaacctgc aaccacacca gcaccagatc gccgctacgt aacgagtcca gcgatttgct
    12121 cttaccgtca gtacccagaa tatgacgctc aatttgcagc acattgctcg ccggtaaagg
    12181 tgcggattgc ggataaccgc tggcatccac acgcagccat aacggctgat cgccactgtt
    12241 ggtcacctgc aaggtggcaa gttgatcgct attcagattg ctggtttgcg ctttctcgcc
    12301 tgtcagcggc tcagcagaga aagtggtttg cgcctgccat ttgcccggta aatcctgaag
    12361 agtacgggca gccaggaaca acgcgttact ttcctgcgtc gatagccagc gttcaccgaa
    12421 cgcctgctgc gaaagtgtgt tcaacaggga gtactgctca tcgggtagca gtttgttctc
    12481 ttccagcaat gagagcatca acgcgctgtc gcgcagtgga ctaccgtaat cacccagcca
    12541 tatccgctca tcactattac gcggcgtttt cagcgccagc acaatcgctt cttcaccacg
    12601 cgttgcatca cccatgattt tcagcgcaac gccaagttgc agcagcggta aaccagaagc
    12661 ggcatctgca cgatgctccc agatttcacg cagcgcaccc agtggtgctt tttgctgacg
    12721 ggccaacacc agcgcagcgt aagactgtac ggcgaattta ctggctttga gattatcagc
    12781 gtacgggatc gatatcatgc ccggatcttg taaatagcgc agcagacgct cattaccccg
    12841 gttaatggcg tctgtcggta cgctgtaacc ctgttcgcct gcgcggacca ggaaatccat
    12901 cacgtaagcc gtcagccagt actcttcgtc accgttttta tcccacagcg caaagccgcc
    12961 gttatcacgt tgcatttgca gcaaacggga aatgccgata tcgaccgatg cacggcgttt
    13021 ctcatcactg tcgcctttga tgcccaacgc ctgcagttgg gctgcattgg tataaagtga
    13081 cgggaacagg ccgctggcgg tttgctcgag acagccgtac ggatacgctt ttaactcttt
    13141 gatataacgt gcgatgttca gcggtggttt gccgctcaac aataactgcc cttccagcgt
    13201 aacaggcgag aagttttgca atccatcagc cgggagcgcc caggtttcac caggctgtaa
    13261 cgccgtaccg taattgaccg tttgtgccgg gaatgccgga cggacgccga ttttccactg
    13321 cttatgctga tcggcaacgg tttcccccgg taacgctaac ccgctaatgg tggcctgaat
    13381 gtcaccatcg ccataacccg tcagagcacg caccgggata aacagcgtag tacgcacccc
    13441 tggcgctaat tcaacaggtg cgggggaatt actcaccagt tcaagcaagc cactggcggt
    13501 cagagcaaca ttaagttttt gcggtttatc ggtaagatta gtgatatcca gcgtcaggcg
    13561 cgaggtatcg ccactcgcca taaagcgcgg catgttaagt tcagcaatca ccggtgcggc
    13621 aacaatcact ttgctttcgt tgcttccgaa gtcatctgcc gtccaggctt gcgccatgac
    13681 gcgcaattca ccgttaaaat cgccaattgg cagtgtaacc gagccttcgc cctgttcatt
    13741 gagcgttacc ggcagcgcct gctgcgcgac aatattgacg tgattgaccg gcggtttacc
    13801 accacgtttc agctcatcac catcaccacc aaaacgcaga gctgccagac gcccctgacc
    13861 ttcaataacc tgaccgtaaa tatcgtaaat gtctgcgcca tagcgtttct gaccaaagaa
    13921 cgcctgccac ggatctggcg tgacgtaatc agtaatattc agcacaccgc tatcaacggc
    13981 agacaccagc acattcacct gttttggcat ctcaccattt ttattgctgg ctttaatttt
    14041 cacggttaat ggctgattgg ggcgcatttt ggtgggggtt tccagcgcca gatcgaggcg
    14101 acggttttca tcaccaagcg gcagatgcaa caccccaacc gcgcgttttg gcgtcgcgga
    14161 gcgagattta tcgccaagac gtaccaccag cgtacttaaa tacagatcat gacgattcca
    14221 ggttttatcg accggaatcg tcagatccag cccttgagcc ggaacatcaa tctcttgcca
    14281 ccacaatggc ccttcactgg actcgaccat cgcataacct ttacccgccg ttggcgcggc
    14341 aatatgcaac ttaatggtat cgccagggcg ataactggct ttatccagtt tcagcgtgac
    14401 acggtcgggt cgcactgcac cacccccgtc gctgttatcc tgccagctat aaccagccca
    14461 gaaacgcaca ctactgaccg cttcattcgg cgctttaact tccagacgat aagcgcccca
    14521 ctctaccggg aaactgacct taccggtttc gtccgctttc agatccagag tttgttcatt
    14581 ttcgatcaga tctttttgat caaactgtga ctgccagcct tcatattccg accagttcca
    14641 gtagtaatcg cggcgttcgc gaatcaggcg cacctgcaag cccgacacgg cttttttcac
    14701 gccttgcgca tcgctataaa cgatgtcaaa agcggcgtta ctgccttcat caacaatggg
    14761 ctgtttcacc gtgctgtcgg tgcgataatc gtaaaccgat ttcgaggcga actgcggacg
    14821 gatccccggc aatgcatcgg caggccagat agcctgctca gcacggcgcg tcaccgggcg
    14881 accgcccgat tccagcaaac taccctggaa aataacctgt aatggagaat gtgtttcctt
    14941 ccactggctt tctgtagaaa cttcgccgcg ccctttatca tccagcgtca actgaacttc
    15001 atccagcgtg cgggaaagat tttcggcagc tatatcgccg aattcaaaac caggtaaggc
    15061 tgacacagct tcacgcagtg ggcgcaggaa aagttgccct tgcaaagtat taccattcgc
    15121 aagtgcacca tacaggtagt accccaccac ggagaatttc acttcatctt tcggcgttag
    15181 cggggttttc tcaccggtca gattcagcgc catacgctct ggcataaaat cttcgacgtg
    15241 gaaatcccac atccgatact ggttatcgcc cgtgttagcg cgaatatgcc acataccggt
    15301 tgccgcattg ctatcgagtg gccaggtaaa gtggtagagg ccattctccg gctgactaac
    15361 gacgctcctg agtacctgcc cgtcgggttt aatcacgtct aacttgatgg gttgatcggg
    15421 caatgcttta ccgtctgcat cacgcagcaa accattgagg atgaccgttt cacccgggcg
    15481 ataaagatcg cgcgggccaa acatgaaaaa ctgtttgcta tagcctggcg cgccagcaat
    15541 gttaaattcc gctaagtcca gcgccgggag ttttaaatcg agcagcgttg tctgaccgtt
    15601 tttacgcgcc agcagtaatg ccgcgttttt atcattttcc agttgcacat gcccctgagc
    15661 gtcactggtt gcctgagtca gagtctgccc tttctcattt aataaagaga cttcaattcc
    15721 ttgcagggcc gcgccgtttt ccagactttg ggtaaagata tccagacgat tgtgataacg
    15781 gtgagctgaa acgccaatat cacttaacgt aaacagcgtc gcgggattac tgtaatcgta
    15841 acgtccagcc tgattcatca cagccagata cacgcccgcc tgctgaagcg gtttgatatc
    15901 gcccagcggc agcaataatt tttcacgggt gttacgcgca ggattgagat caaaccgtcc
    15961 ggtgtagacc agatccgcca tctgcagcag tttatctgac tgccagttcg ccagcgaatt
    16021 gcggtattcc cactggctaa tgaaagcggg cagtgattct ggtttcacac ggaagaagtt
    16081 aacatcgaca ttgttaacgt taagcgccat taccggcagc ccttcaacga ccttaccagg
    16141 aagcaacgaa ccacgactgg caaaaccgac gctgggttgg atatcgcggg tagttatagt
    16201 tttttcgtca tctttactaa aggttgcgtt attgagcgct ttgacctcct tgccaatagt
    16261 gacgatcaaa tcacgtttcg gttcgaggtg gcgtaaacgc agctctttaa gattatctga
    16321 cagctcccag gcaccatcca ctttgccgct ttttttatcg acgacatgaa taacgcgtga
    16381 gaaatcctga tccgggtcga gagggatgga gaacgtcagc accagcgtgg ctgcgccatc
    16441 gagttgaact tcagagagat ccagcaatgt cagcgcctta ccggcactct gttgggccag
    16501 tttttgtcgc tccggcgcgg aaagttttgc tgaactcacg ttttctgaag aggccgcttt
    16561 agtaacttca gaaggcgcat cttttttcac cgctgttggc gcgttatcgt tgttgtcgca
    16621 ccctgccagc gccagcatta gcatgcaggc ggctacgcgt aactttttca tttttcatcc
    16681 ctgccacaat ggcccgttag caacgtcgaa taattattat gcgtgagaat ttacgttatg
    16741 taattcagta tcacgcccat acaatccgaa ttttagtgag aattgtggct cagacagcca
    16801 aatcagcctc ttttgcttgt cgcttttcac taaacatccg acaatttagc ctaaccccgg
    16861 caaaaatgga gatgcctatg tccacgacat ggtttgtagg agccgactgg ctcgccgaac
    16921 atattgatga cccggaaatt cagattatcg atgcccgcat ggcgtcgcct ggacaggaag
    16981 atcgtaacgt tgctcaggag tatctgaatg gacatattcc cggcgcagtg ttttttgata
    17041 tcgaagcgct ttctgatcac acttccccgc ttccgcacat gctgccgcgc ccggaaacgt
    17101 tcgccgtggc gatgcgtgaa ttaggcgtta accaggataa gcacctgatt gtctatgacg
    17161 aaggtaatct tttctcagcc ccacgagcat ggtggatgct gcgcaccttt ggtgtagaga
    17221 aagtgtcgat tctgggtggt ggacttgcag gctggctgcg tgatgatctg ctattagaag
    17281 aaggcgcagt agagctgccg gaaggagagt ttaacgccgc gtttaatcct gaagccgtgg
    17341 tgaaagtaac cgatgtatta ttggcaagcc atgaaaatac ggcgcaaatt attgatgccc
    17401 gcccggctac acgttttaac gcagaagttg atgaacctcg cccaggttta cgtcgcggac
    17461 atattcccgg tgcattgaac gttccgtgga cggaactggt acgtgaaggc gaactaaaaa
    17521 cgaccgatga actggatgcg atattttttg gtcgcggtgt cagctatgac aaaccaatta
    17581 tcgtcagctg cggttctggt gtaacagcag ccgtggtttt gttggcactt gcgacgttgg
    17641 atgtgccaaa cgtgaaactg tacgacggcg catggagtga atggggcgca cgggcagatt
    17701 taccggttga gcccgtaaaa taagcttttt acgggcaata aaaaaccgcc gaatttggcg
    17761 gttttttatt gctggtctgg ttcgcggcct ttccagcagg ttgtattacc gtagtaatgc
    17821 aagcgcgtct cagcggacac aatactcgcc agtaactctc tttttgtcaa gtaaaagaga
    17881 gttattattg ttctgttagt gtattatcca ctgcggccct ttccgccgtc tcgcaagcgg
    17941 gcgctggctt aaggaaagga tgttccgcga ccgttagggc aagcgcgtct cagcgcttgc
    18001 tatcgcggca atatcgccag tggtgctgtc gtgatgcggt cttcgcatgg accgcacaat
    18061 gaagatacgg tgcttttgta tcgtacttat tgtttctggt gcgctgttaa ccgaggtaaa
    18121 taataaccga agtctcttcg gcgacaattt actggtggtt aacaaccttc agagcagcaa
    18181 gtaagcccga atgccgccct ttggggcggc atattttaga ttatccggtt ctgtttaaag
    18241 tcacgcaaaa aaccacccca gcgacgttca tagaatggcg caatatgttc ggtaataaag
    18301 tggctaattc ctttttcccc ttttttcacc tgacaaatat cgatgggttc atcaccaggt
    18361 aatgtatcgg tcgctacact tcccgccacc tgaataattt cttcgatatc accatcggct
    18421 tcaatgccaa taagtaaatt aggctgtgcc tcttcgttct ctttaattga acaaataaaa
    18481 gcacgcttca ccggcttaat ggttttaaat aaggtggtaa gtgaatcaat catttgcgct
    18541 ggcggctctg cgacttccga taatatcagc gattcaccgc cttccagtac ttcctggcta
    18601 ctcagcggat tcccttcttc accaatcaac aaactgattt cacgcggcat aaactcttta
    18661 ccggttggca gtttggcatt aaggaagagc gtttcgccaa gggtcatctc aaacaatgtg
    18721 cgaacgggca ttacgacaaa tgcctgttcg tcttcaaccg cctgttgaag tgcttctaac
    18781 gaggtaaaaa aaggaatgac gctggtgccg tcttcttttt cccagtgctg taaatcaagc
    18841 gcgctatctt caactactgc ctcgccctgc gccgccgtac caggcaccca gacggtggat
    18901 tccagtagag tacggaaaaa ggccgggcgg tgcgccggtt cggttgctgc tttttccagc
    18961 aggtcttcaa gttcgttttt tgtttcggac ataagaacca caattcattc aacgttcggg
    19021 gagcaagtgc ctgatgcgcc acgcttatca ggcctacaag ggagtcgcaa tctattggat
    19081 tttcgcaatt tgtaggccgg ataaggcgtt tacgccgcat ccggcatatt agtttacgcc
    19141 gttaacagat tagcaatcgt gcgcacacca agtcccgtag cacccgcaga ccactgttca
    19201 accggcgctt tacggtaagt cgccgagcag tcgatatgca gccagccttg ctgatagttc
    19261 tcaacaaagt gcgacaggaa gcccgcagcc gtacttgcac ctgccggata cgccgcgctt
    19321 ccggtattgt tcagttcggc aaagttagac ggcagctggc tgcggtggaa ttccgccagc
    19381 ggcagacgcc agaacggttc gttctcttgt gaggcactcg ccagcaaacg accggcaagc
    19441 gcatcgtcaa aactgaacaa cgcgtgataa tcattaccca gcgcagtttt cgccgccccg
    19501 gtgagggtcg ccgcatcaat gatcagttcc ggtttctgcg cactggcatc aatcaggcca
    19561 tcggccagca ccagacgccc ttcggcatca gtgttcatca cttcaacttt tttaccgttg
    19621 cgataggtga tgatatcgcc cagcttgaac gcattgccgc tgatcaggtt atccgcacaa
    19681 cagaggaaca gcttcacgcg cttgttcagt ccgcgcgtaa tggcaaatgc cagcgccccg
    19741 gtaaccgttg ccgcaccgcc catatccgac ttcatcgagt ccataaacgc ggtctgtttg
    19801 atgctgtagc cgccggaatc aaaagtgatg cctttaccaa ccaggcacgc gtacactggc
    19861 gcttctttat cgccagttgg gttgtaatcc agcgccagca ataccggaga acgttctgaa
    19921 ccgcgcccga cagtgtgcag ccccatataa ccttgctcac gcagatcttc gcctttggtg
    19981 atccggtaag tcacacgatc ccctgcgacg ttgcagatca gatcaacagc acgctgtgcc
    20041 agttgcgatg gtcccagctc ttctgccggt gcgttgatgg tgtcacgcac ccagtcaatg
    20101 atcatcaggc ggttatccag ttcctgacgc tgggcatcgt ccagctctgg ccactccact
    20161 ttacgtgtgc ctttcggggc tttgtaacct tgccagaatg cccagcagcg atccgcatcc
    20221 cagccttcac cgcttaactg aacatgctta atgcccagac catcaacctt gcgcgccgca
    20281 cgctggatca accccagatc gtctgccccg ttcaaatgca gggtaatgcc atcattatta
    20341 atgctgtaag ttgctttttc tccccagcgc acgtcggcag gttgggtaga aagggtaatt
    20401 ttcatcgctt ctgtcatttt agttatcctt attcgtttaa cagcaaaacg ggctaccccc
    20461 aggcagcccg ttcattcact cggcttcgtc cagccagacc aacaaaatcg cttcgaggat
    20521 tttctcgttc gatgcctgcg ggtcgtcgtc gaaatcttcc agagcgcaaa tccactgatg
    20581 catatcggtg aatcgaaccg ttttcggatc aatatcggga tacgcatcgt acagtgcttc
    20641 gccaatttcg cggctatcgg tccacttaag tcccatacta acctctgtta atgctcacgc
    20701 gcatggttga tagtgtaacg cgggatttcg accactaaat cttcgtcggt aacgcgcgcc
    20761 tggcagctta aacggctttc cggctccagc ccccaggctt tgtccagcat gtcgtcttcc
    20821 tgctctgagc tttccggcag cgagtcaaaa ccttcgcgaa cgatgcagtg gcaggtggtg
    20881 caagcacagg atttttcaca ggcgtgttca atctcgatac cgttacgcag cgcaacatcg
    20941 agaatggttt caccgctatt cgcttccaga acagcgccat cagggcagag atcctgatga
    21001 ggcaaaataa caatctttgg catattaaac ctcgtccacg gaatggcctt tcagcgcacg
    21061 acgaaccgac tggtccatgc ggcgagcggc gaaatcctgg gtttgtttgt ctacgttttt
    21121 aatcgcttgt tcgatggcgt caacatcatc gccctgcgcc acttcactca ggtgagcggc
    21181 agcatcgtcg ataacctgac gttctgcggc gcttaacagc gcggcatcag cagccagcgc
    21241 accgtgcaga ctttccagca cacgcgccgc ttctactttt tgttctgcca gcattcgggc
    21301 ttttacgtcc tgctcggcat agctcattga gtctttgatc atcgaggcga tttcgctatc
    21361 ggtcaggcca taagacggtt tgacctgaat agacgcctca acgccggtgg atttctccat
    21421 cgccgtcacg ctcaaaagac cgtcggcatc gacctggaac gtcacgcgaa tatgcgcacc
    21481 gccagccggt agcgccggaa tacctcgcag cgcaaaacgc gccagcgagc ggcagtcctg
    21541 caccagttcg cgctcaccct gcatcacatg gatagacatc gccgtctgac catctttaaa
    21601 ggtggtgaaa tcctgggcgc gggccaccgg aatcgtggta ttacgcggga tcactttctc
    21661 caccaggccg cccatcgttt cgaggcccag cgacagcggg atcacatcca gcaacagcat
    21721 ttcgctgtcc ggcttgttac ccaccagaat atccgcctga atcgccgcgc caatagcgac
    21781 gactttatcc gggtcgatag aagtcagcgg tggacgaccg aaaaattcgc ctactcgttc
    21841 acgcaccagc ggcacgcgag tagaaccgcc caccatcacc acttccagca cttcatcagc
    21901 ttctacgccc gcatctttca gcgcgcgacg acaagccagt aaggttcgtt ttaccagtgg
    21961 cgcgatcagt tcattgaatt gttcacggct gatttcgccc tgccagcccg caacgttaac
    22021 ggtcacagag tccgcatcgc tcagcgcgat tttggccgca atggcagcat cgagcagttc
    22081 acgctgaacg cggttatcgc tacgatcagg aatgcccgcc tgctcgcgaa tgtaatccgc
    22141 cagcagatgg tcgaaatcat cgccgccgag cgcggaatca ccgccggtcg ccagcacttc
    22201 aaacacgccg cgacttaagc gcagaatgga aatatcaaac gtcccgccgc cgaggtcata
    22261 aacagcgatc acgccttcct ggccggaatc cagcccgtag gcaatagccg cagcggtcgg
    22321 ttcattaagt aagcgcagaa catgcagccc cgccagacgc gccgcgtctt tggtgccctg
    22381 acgctgggca tcatcaaagt acgccggaac ggtgataacg acaccatcca gctcgcctgc
    22441 cagggcttca gttgcccgcg ccgccagtgc tttgaggatg tccgcagaaa cgcgcaccgg
    22501 gttcagcagc cccgccgccg tttcaatcat cggcaggcca ttctcgctgg cctggaattg
    22561 ataaggcaga tgcggatagc gttgctggat atcagccagc gagcgcccca tcaggcgttt
    22621 aacagaacta atggtgttgg cggtgtcgag cgctgcgtta gtacgcgcgt cataacccac
    22681 cgaatgccct tgctgttgat agtgaacaac agacggcagc aggtgacggc cttcatggtc
    22741 agctaacgtt tcggcctgac cgctgcgcac tgttgccacc agcgagttgg ttgtgcccag
    22801 gtcaataccg gccgccaggc gacgctgatg cggcgcagca ctcaaaccag gttcactaat
    22861 ttgtaataag gccatgttta gcttccagaa attaaaaatc gagcagtttt tcttcgagtt
    22921 gttcggcact gctccgcagt ttatcgagaa aacgcagctt acgcacagta tccgccgccg
    22981 cgtcccacgt ctcgttgtct aactgttcaa ccatcaactg atggcgggta tcaaacatct
    23041 ttttcacacg tttgataaag ctttccagcc gcgcttcatc tttcgcctgt tcgatctcgt
    23101 ccagttcttc gcgcagttcc agctgttcca tcaggaacgc ggtgtcgcgc acagtatgct
    23161 gctcgctggc gagatcaaaa ccgtgcaaag aaagcaaata ttccgcgcgc attaacggat
    23221 gacgcagcgt ttgccaggcc tggttaatgg tcgcggattg ctgtacggcg gcgagttgtt
    23281 ccgcctggct tccgctggcg aatttatcag gatgatactg acgttgtaga tcctgaaaac
    23341 gcaggctcag cgcctgggta tcgagttgat aacgggcagg caagccaaag agggtgaagt
    23401 aatccataac attctcaggg ctgcgatccg ccgcgataac ggcgaatcag gtaaaacaaa
    23461 ccccacgcgc aggcgaccac ggtggggtta tcggtatgcg catcaaacgt ggaagctttc
    23521 gccgcaacca cactcatctt tgacgttcgg gttggtgaat ttaaaccctt cgttaaggcc
    23581 ttctttgacg aagtccagct gcgtaccgtc cagaaattgc aggcttttgc catcgaccac
    23641 gactttcacg cctttgtctt caaacacgat gtcttccggc gtcggttcgt caacaaattc
    23701 cagtacataa gccatacctg aacacccgga ggttctcacg cccagacgca ggccaaaccc
    23761 tttaccgcgg ttagccagaa aggtatttac tcgcgctgct gcactgtcgc tcagtgtaat
    23821 cgacataacc aaacctcaac tcttattttg cttcacgttt gcttttatag tccgcaatgg
    23881 cggctttgat cgcgtcttct gccagaatag aacagtgaat tttcaccggc ggcagttcaa
    23941 gttcttcagc aatatcggtg tttttgatcg cctgcgcttc gtcgagagac ttccctttca
    24001 cccattcggt gaccagggag ctggaagcga tagcggaacc gcagccgtaa gttttaaaac
    24061 gcgcgtcttc aatgatacct tcatcgttga ctttaatctg caacttcatc acgtcgccac
    24121 aggccggtgc acccaccatg ccgctgccga cgttctcgtc gttgttgtca aaggaaccca
    24181 cgttacgcgg attctcgtaa tggtcgataa ctttttcgct gtaagccatt ataaattctc
    24241 ctgattccga taccgattaa tgatgagccc attcgatgct gttcagatcc acgccctgct
    24301 tgtacatttc ccacagcgga gaaaggtcac gcagacgacc gatggattta cgaactaact
    24361 cgatggtgta gtcgatctct tcttcagtag taaaacgacc taaagagaaa cggatagagc
    24421 tatgtgccag ctcgtcgttc agccccagcg cgcgcagcac gtaggacggt tcgaggcttg
    24481 ctgacgtaca ggcggaacct gaagaaaccg cgaggtcttt cagcgccata atcagcgact
    24541 caccttcaac gtagttgaag ctgacgttga gaatgttcgg cgcaccgtgt tccaggtcac
    24601 cgttcaggta aacttcttcg atatctttga tgccgttcca cagacgatta cgcaggccgc
    24661 gcagacgttc catctcggtc gccatctctt cttttgcgat gcgataggct tcgcccatac
    24721 cgacgatctg gtgaacaggc agagtgccgg aacgcatacc gcgctcgtga ccgccgccat
    24781 gcatttgtgc ttcgatacgt acgcgcggtt tacgacgcac atacagcgca ccaatacctt
    24841 tcgggccata gattttgtga ccggagaaag acatcaggtc aactttcaac tggctcagat
    24901 cgatcggcag tttgcccacg ctctgggttg catcaacgtg atagataatg ccacgagcac
    24961 ggcacatttc gccgatagcc gcgatatcct gcaccacgcc gatttcgtta tttacgtgca
    25021 tgatggaaac aaggatggtg tcgtcacgca tcgctgcttc gagttctttc aggtcgataa
    25081 taccgttacg ctgcggtgcc aggtaagtga cttcaaaacc ttcgcgctcc agctgacggc
    25141 aggtatccag taccgcttta tgttcggttt tactggtgat gatgtgcttg ccttttttct
    25201 gataaaagtt ggctgcacct ttgatcgcca ggttgtcaga ttcggttgca ccagaggtaa
    25261 agacgatttc acgcggatca gcgccgacca gatcggcaat ctgattacgg gcgatatcta
    25321 ccgcttcttc agcctgccag ccgaaacggt gagaacggga ggccgggtta ccaaaggttc
    25381 cgtccatcgt cataaactgc atcattttct cggcaacacg cgggtccacc ggcgtggttg
    25441 cggagtagtc gagataaatc ggtaatttca ttgctctata aactccgtac atcactcaat
    25501 gcaaggaacc aggctaccgg ctggatgtac gaccgtgttt acggagtgtt tagcactccg
    25561 gcctgattct gaattctttt tattaagcgc gtaacttaac gtcgatcgcg tcttgtgtgc
    25621 gggtgcgtgg cgcgtcgtga gtatgctgac gaccagacac atccagcact tcctggttat
    25681 taaccagttc gcctaaagta atgttattga gaaaaccggt aagacggtcg ctcaaatcac
    25741 gccacagcgc gtgggtcagg catttatcgc cgccctggca gccgccttta ccctgacaac
    25801 gggtggcatc tacagattcg tcaacggcgc taatcacctc gccaacggcg atgctgctgg
    25861 catctttgcc taacagataa ccaccgcctg gtccacgtac gctggaaacc agaccatttt
    25921 tacgcagacg ggaaaacagt tgttccagat aagaaaggga aattccctga cgttcggaaa
    25981 tatcagccaa cggtaccggg cccgcttcag agttgagcgc aacgtcaagc attgcggtca
    26041 cggcatagcg ccctttagat gtcagtctca tgtcttactt cacctcaaac tcgcccctgc
    26101 ccggggtttt ttattgtaaa agtgggggta ttgcatagca gggtcaagtc tgacattccc
    26161 gagtaaattg gtcaactatt tacttgactg atttagtcgg gtatttaacc ttcagtgcca
    26221 tttttttatc gtggcgtttg tacctgttgt cggatgcggt gtgaacacct tatccgacct
    26281 gggattccgt gcacttacag gcctgataag acgcgttaag catcgcatca ggcaaccact
    26341 gttattcggc cttgttacct ttattctgct gctcaataga agccagaatc ccgcgcagga
    26401 tattcaattc ctggctttcc gggcgcgcac gggtaaacag acggcgcaat ttattcatca
    26461 cctgtcccgg atggttttca cggataaaac cgcttgccag cagcgtttgc tccagatgac
    26521 cataaaaacg ctccagatca tcgaccagcg gatacggcgt ctcttcatgc tcgacctgct
    26581 cgccattttc ctgagttgcc agccaggcca tgcgcacttc ataggcaata acctgaaccg
    26641 ccatcgccag gttcagcgaa ctgtattccg ggttagccgc aattgcgaca tgataatggc
    26701 atttctgcaa ctcttcattg gtcaagccga cgcgctcgcg accaaacacc agcgccaccg
    26761 gggtatttgc cgcttcagcg acgcttttca gaccgcattc gcgcgggtcg agcatcggcc
    26821 acggcagcgt gcgggagcga gcgctggtgc ccaccaccag actacaaccg gctaacgctt
    26881 cgtcgagcgt atcgacgatg tgagcattac caatcacatc gctggcccct gctgccaggg
    26941 caatcgcctg ggagtcaggt ttcactagtg gattaaccag ccacagattg gttaatccca
    27001 ttgttttcat ggcacgggca acagaaccca tattgccggt gtgtgacgtc tccaccagca
    27061 caattcgaat attttgcagc attgtctttc ttcatctaaa gattattcac gcatcttatc
    27121 ataaaacgaa gacagatgcc gatctcgctg ctatactctg cgccgttttc ccgttcttta
    27181 acatccagtg agagagaccg atgcatccga tgctgaacat cgccgtgcgc gcagcgcgca
    27241 aggcgggtaa tttaattgcc aaaaactatg aaaccccgga cgctgtagaa gcgagccaga
    27301 aaggcagtaa cgatttcgtg accaacgtag ataaagctgc cgaagcggtg attatcgaca
    27361 cgattcgtaa atcttaccca cagcacacca tcatcaccga agaaagcggt gaacttgaag
    27421 gtactgatca ggatgttcaa tgggttatcg atccactgga tggcactacc aactttatca
    27481 aacgtctgcc gcacttcgcg gtatctatcg ccgttcgtat caaaggccgc accgaagttg
    27541 ctgtggtata cgatcctatg cgtaacgaac tgttcaccgc cactcgcggt cagggcgcac
    27601 agctgaacgg ctaccgtctg cgcggcagca ccgctcgcga tctcgacggt actattctgg
    27661 cgaccggctt cccgttcaaa gcaaaacagt acgccactac ctacatcaac atcgtcggca
    27721 aactgtttaa cgaatgtgca gacttccgtc gtaccggttc tgcggcgctg gatctggctt
    27781 acgtcgctgc gggtcgtgtt gacggtttct ttgaaatcgg tctgcgcccg tgggatttcg
    27841 cggcaggcga gctgctggtt cgtgaagcag gcggcatcgt cagcgacttc accggtggtc
    27901 ataactatat gctgaccggt aacatcgttg ctggtaaccc gcgcgttgtt aaagccatgc
    27961 tggcgaacat gcgtgacgag ttaagcgacg ctctgaagcg ttaatgactc aggcgggtga
    28021 tatcactcac ccgccctcgc ctttcaggcg ctattccgaa atacttcctc accgctttac
    28081 tttctttcgt cactctccca ccatctttcg ccgattaatg gatgataaag aagtcgcaag
    28141 gaaaggttat ggcgctgcca gtgaacaaac gcgttctcaa aattctgttt attctctttg
    28201 ttgttgcctt ctgcgtttat ttagtgccgc gcgtcgccat caacttcttc tattatcctg
    28261 acgataaaat atacggtccc gacccctggt cggcggaatc cgtcgaattt acggctaagg
    28321 acggtactcg tctgcaaggc tggtttattc cttcttcgac gggccctgct gacaacgcca
    28381 tcgcaaccat cattcatgct cacggcaatg ccggaaatat gtccgcccac tggccgctgg
    28441 tcagttggtt acccgagcgt aatttcaacg tttttatgtt tgattatcgc gggtttggta
    28501 aatcaaaagg cacgccgtcc ctggccggat tgctggacga tacgcaaagt gccatcaatg
    28561 tggtgcgcca tcgcagtgat gtaaacccac aacggctggt gctgttcggg cagagtattg
    28621 gcggggcgaa tattctggat gttattggtc agggtgatcg tgaaggcata cgtgcggtga
    28681 tcctcgactc cacctttgcc tcttatgcga ccattgccaa ccagatgatc cccggcagtg
    28741 gctacttact tgatgagagt tacagcggcg aaaattacat cgccagcgtc agcccgatcc
    28801 cgcttttact cattcacggt aaagctgatc acgttatccc atggcagcac agcgaaaagt
    28861 tgtatagcct ggcaaaagag ccaaaacggt tgatcctaat cccggatggc gaacacattg
    28921 atgctttttc cgatcgtcac ggcgatgttt atcgcgaaca gatggtggat tttatcctta
    28981 gcgcattgaa tccgcagaac taacccatga tcgctagcac gataatcatt cacaaaacca
    29041 ccttaagaca tgctaatcca ctggtcagaa cagtttaaga tgagaaaaat tctgtgatgc
    29101 ttgccaacat ttctgatgat tagcattccc ttcgccattt ccctgagcaa actttagcta
    29161 ttcttatcaa ttatgcttat gggagatcta cagatgatgc ctacgcttgc tccaccatct
    29221 gtcctttcgg ctccccagcg ccgctgccag atcttgctga cgctctttca gccggggttg
    29281 accgccacca cggcaacttt cagcgagctt aatggtgtgg atgatgatat cgccagtctt
    29341 gatatcagcg aaacaggacg ggagatcctg cgctatcatc aactcacact gacgactggt
    29401 tatgacggca gctaccgggt tgaaggtaca gtgcttaacc aacgtttgtg tttatttcac
    29461 tggctacgac gtggtttccg tctgtgtccg tcatttatta ccagccgatt cacccccgcc
    29521 ctgaagagtg aactgaagcg gcgcggaatt gcgcgtaact tttacgacga taccaatcta
    29581 caagcgttag tgaatctctg ctcccgacgg ctgcaaaaac gctttgaaac gcgcgatatt
    29641 catttcctgt gtctgtattt gcaatattgt ttgttgcaac atcacgctgg aattacgcct
    29701 cagtttaatc cgctccaacg tcgctgggcg gaatcctgcc ttgaatttca ggtagcgcag
    29761 gaaattggac gccactggca gcgtcgggcg ctccagcctg taccgcctga tgagccacta
    29821 tttatggcac tacttttttc catgttgcgg gttcccgatc cactgcggga tgcgcatcag
    29881 cgggacagac aactgcgtca gtctatcaaa cgtctggtaa accattttcg tgagctggga
    29941 aatgttcgtt tttatgatga acaggggtta tgcgatcagc tttataccca cctcgcccag
    30001 gcgttaaatc gcagtttgtt tgccatcggt attgataaca ccctgccgga agagttcgcc
    30061 cgcctgtatc cacgcctggt gcgcaccacc cgcgcggcgc tggccggatt tgaaagtgaa
    30121 tacggcgtcc atctttctga tgaggaaagc ggtctggtcg cggtgatttt tggtgcctgg
    30181 ctaatgcagg aaaacaacct gcatgagaaa cagattattc tactgaccgg gaatgatagc
    30241 gagcgagaag cgcagattga gcagcagcta cgcgaactaa cgttactgcc gctcaacatt
    30301 aaacatatgt cggtaaaggc atttttgcag acaggcgctc cgcgcggcgc ggcactgatt
    30361 attgcgcctt ataccatgcc gttaccgctc ttttcaccac cgctgatcta tacggacctg
    30421 acgttgacaa cacatcaaca ggagcagatc cgcaaaatgc tcgaatcagc atgagggaac
    30481 aactttcggg cgcaaaaaca ttgccggaag cgccaccagc gccatcaccc agaacacgcc
    30541 gtggccgaga tattgataca ggaaaccggc gaaaacggtc atgatggcga tactgccgcc
    30601 catcgcgacg gcagagtaaa ccgcctgtaa acggatgact tcgctaccct ggcgagcagc
    30661 aatatagcgc atggcagcca ggtggcagac cgtgaaggta ccgcaatgca gaatttgcac
    30721 cactatcaac cacggcaacg ccgtcgttgc tcccataatg ccccagcgca ctacgccgca
    30781 aatcgccgag atcaacagca tatcgcgtgc actacaacgg cggaaaagtt tattactcag
    30841 cgcaaaaata atgacttccg ccaccacgcc cagcgaccac aaatacccca ccgccgaggc
    30901 cgagtagcca gctgcctgcc agtaaatggc gctaaaaccg taataggccg catgtgcccc
    30961 ctgcaataaa caaacgcagg ccagaaagcg ccagttctgg cgaaccaacg ccaaccacgc
    31021 agaccagccg gtgctctcct gctggcggct tgccccttgt ggctgaatcg tcggacggat
    31081 gagaaagccg agcagcatgg atgccacgcc caacgtcaac agcgcgagga tcacccgata
    31141 atcaaacata ctgaccagtt tgcccgtcag cgccgagcca atgacaaacg ccaccgagcc
    31201 ccacagtctc actttgccgt aatcaagcgg gaactgcttt tgccacgtat tcgccagtgc
    31261 atcggtcaac ggtaccagcg gtgagaaaaa gaggttaaag ccaatcatca ccagcattag
    31321 ccacgctacg tgcgcccccg cccagaaggc gacagcaaag agaagtgtca gcagtgccag
    31381 cacgcgcaag gcggaaatca ggcgggaagg atcgctgacg cggggcgcga tgagcaaact
    31441 cccgaggaaa cgggcaacca gacccgcccc caataacagg ccgatggttt ctggcgttaa
    31501 accaatccct ttaagccaga cgctccagaa aggtagaaaa atgccgtaac taaaaaagta
    31561 tgtgaaatag ccgagcgcca accagcgcgt ggattgcaaa accatgagtc cctcccgtca
    31621 ggaggcgtta gtctggggtt aatcgaatgt tggtgcaagt tgaaaaataa gaatattcga
    31681 gcctgcttcc tgacggttat cctgcatatc aggaaatgac aaaaaagtgc ggcccacaac
    31741 acaacatgta aaaaagaacg atgaaacagg tgaaaatcgc aatttattga actgacaaac
    31801 tcatgtgaga cggataaggc gtgcatgtcg cattcggcag gaaacaataa gcacattgtc
    31861 agccatctgc gttatgccgt tataccagaa acgaatatct caacgcctgg ggcgtggcga
    31921 ggtaccagag tgtgtattac gtttaaatca catcatcttg caaagggatt ggttatgaac
    31981 tcactacgtt attttgattt tggcactgcc cgccccgttt tattattaat tgcccgtatc
    32041 gccgtggtct taattttcat tatttttggt tttcccaaaa tgatgggctt tgacggtacg
    32101 gtccaatata tggcctcgct gggcgcgcca atgccgatgc tggcagcgat tattgcggta
    32161 gttatggaag tgcccgccgc gatattaatc gtgcttggct ttttcacccg tccgctggcg
    32221 gtgctgttta ttttctacac gctgggtacg gcggtgattg gtcaccatta ctgggatatg
    32281 accggcgatg cggttgggcc aaatatgatt aatttctgga agaatgtcag tatcgctggc
    32341 gcattcctgc tgttggcaat taccgggccg ggggcgattt ctctcgatcg ccgttaggaa
    32401 agatgccgga tgtggcgtga acgccttatc cggcatttta taaattacag caacgcaacc
    32461 cgcaacgaca tctctgaaat tgttgattcc cctggtgcaa gcgcaatgag gtcaccacct
    32521 tccgggcgat gatggtcatc cggcgcatga ctcatcggtt ccagacagaa gaaatcaaac
    32581 gcatatcctt tatcaaacgc agggtctgaa acaaagatga aataacacgg tgcaggtggc
    32641 gtcgtttcca tgatgatggc atacccctcc tgcggctgct cgatgcgggc cttaccattc
    32701 catccggcaa aaccattgtt tacccactgg cgcggcaacg gcgcgagctg gttaaaatcc
    32761 agctcctgcg gtagctgctc gcaaaactca cccgccagcc actgctcccg ctccagccag
    32821 taaccgctcg cctgtgcctg aatccgcgtt tgcggcgaca acggaaaata aggatgccag
    32881 ccggtaccaa atggcagcgt ctctgcccct tgattggtga cagcgagcgt caccgtcagc
    32941 gtatccgccg ttaaatgaaa tgcctggctt acccgatagt gataaacacc gctgagatgt
    33001 tcatacacca gacacaagct atcatcgctg cgcgagacac attgccactc gcccaaccag
    33061 ccatcgccgt gtagatagtg cgcatcccac tcaacattcg gttgcagttg atactcacgc
    33121 ccctgccaga caaaacggtt gccgctcacc cggtttgcaa atggtaccag cggaaaacaa
    33181 gaggcatcgg ttgccacacc gcttttttta ccaggacgta gcagcggcgt cgtatcgcgc
    33241 cagaagcctt cgattacgcc gccctgatca gaaacgtcca gctttagcgg cccgtgagat
    33301 aaggtataga tggtcatccg cagactcctt aatccgggaa attaatcaca actttcccac
    33361 atttgccgct tgccatcagt gcataagcat ctcctgcctg ttccagcgag aaacgatggg
    33421 taatggcgtt acgcggccac agtttccagt ccgttaaatc gtgggcgcat ttttccatat
    33481 ggaacaaact ggtcacccag gagccaataa tccgccgttg atggtgcatc aggtcagcgc
    33541 taacctcgaa ctcgactttt ccggtttcac caatgtaaac cacgcgtccc cagtcagctg
    33601 tggattgcag tgccagtaag cgacctgcgg cattaccgga acaatcaagc gcaacatccg
    33661 cgccgccgtg agtgagttcg gcgattatct gcggcagacc ttcggtggtc gctaaatagc
    33721 cgtgatccat cactcctaac tgtttcgcca tcgccagacg ttccggcagc atatcaacgc
    33781 cgatgatcct ttttgcacca cgacctttcg ccagcatcat cgccatcatg ccgaccggcc
    33841 ccagaccgac caccagcacg ttatcgctac cggaaacttc gccgcgcaaa atcccttcat
    33901 acgctgtacc aacgccgcaa ctgataaacg caccatcttc atagctcagc gcgtccggca
    33961 gcaggatcag atctttttct tccgccagca ggtattcagc atggccgccg tcacgctgcc
    34021 agccgtaagc cgcttttcct tcgccagtac aagaaatagg aaaaccgcga cggcagttcg
    34081 ggcaaaaacc acagccagaa atgtgataca ccagcacgcg gtcgccctct ttaaaatggc
    34141 ggcagccttg ccccatcgcc acaatctgcc cgcacggttc atgaccgttg ataaatccct
    34201 ggtataacgg tttatcgggt gccgccgccg tagcgcggtg ctggtggtag atatagtgga
    34261 catcgcttcc gcaaatcccg gaggatttca ttttgatcag tacctggtta atccccggcg
    34321 tcggcaccgc aacttcccgc agatcgacgg tcgaatttcc tggtaaataa gctgccagca
    34381 tcgttttcat aaatccctca ttaacaatac gattaatttt catccctgcc gcacccgcgc
    34441 caggggcgtt aaggtttagc gtttcgcttt actactacgc tgggtcagca agatatttgc
    34501 cagcaccgcc accacaatga tgacgccgcg caccacctgc tggaaaaagg agttaatccc
    34561 gagcagcacc agaccgttac cgattagcgt aatcaccagt acgccaagca gcgtaccgaa
    34621 caacgagccg cgaccgccgg aaagcgccgt accgccgacc acgaccgcgg cgatgacgtc
    34681 aaactccaga ccgtttgcgg cacctgcgtt accggaaccg aggcgcgccg ccaacaaaat
    34741 gccggtcacc gccgctaata atcccgaaag ggtaaaaata agaatgcgta cccgacgaac
    34801 gttaataccg cacaactgcg ctgcagtggc attaccgcca acagcaaaaa ccgagcgccc
    34861 gaaggcggtt ttgcggctaa tgaacacaaa cagcgcaaat aacaccatca tgatcagcgc
    34921 ggataccggc acaccgagaa attgtccgcc cagccagtcc agcacttcgt tttcgttaat
    34981 tggcactggc aaggcgttcg tcataaacag ccccattccg cgcagggcgc tccataaacc
    35041 cagcgtggca acaaaacttg gcacgttaaa cacgccgcgc agcaccccgg cgagcgttcc
    35101 catcagcgca cctaacagca acaccagcag acaagcaatc gccagcggaa cttcaaattg
    35161 cagcaaaaac gccaggcaca ctgaaacaaa agccaccatc ggcccaacgc tgacatcaat
    35221 ttcaccggag ataataatca gcgtcatcgc ccaggcggcg atcccgatgg tggcggcatc
    35281 gcgcagcacg ttcatttggt tattcaatga gataaagcca ggcgcattca gggagaagac
    35341 cagataaaga atggcaatca ccaccagcaa accgatctca ttaatatggc gactgacaaa
    35401 ttgtttgagc gagacgctct tgccctgcgg caatggtaat gacgaagcag acatcttcag
    35461 gttcctcggt aaaattcatc agtggacaga atggcggaca tcagctcatc cacattgacc
    35521 ggcgagtgaa actcctgcga gaacgtgccg tgctgtaata acaggatgcg gtcacacacc
    35581 agcggcaact cctccacttc actggagata aacaccacgc tttttccttc ggcagccagc
    35641 tcgcggacaa tacggtaaat ctgctgtttg gcttcgatat cgacgccgcg cgttggctcg
    35701 tcgagcaaca aaatctggct ggcggcatag acccaacgac cgatcaccac tttttgctga
    35761 ttgccgccag aaagcgtgcc gatgggcgtt tcgctactgg cggccttgac cgtcatccgc
    35821 tgcatcacct cttcggtcag gcggcggatg gtggaccatt gcagcacacc gttggcgctg
    35881 attttttgcc gattggtcag cactgtattt tcgtcaacgc ccaaccaggg aatgatcccc
    35941 gcttctttgc ggttttctgg cgtatagcca atgccgcgtt tcagcatgtc gccgtaatcg
    36001 gggcgcatga ttttctcgcc gttgataaca atttcgccct gttcatacgt ctccagacca
    36061 acaatcgcct tcagcaattc actgcgccct gcccccagca gaccagcaat gccgagcact
    36121 tcgccacgac gtagcgtaaa actgatatcc tccagcttgg gcttatggcg taacgcacgg
    36181 acttccagca cggcttgatc cataatttcc tgaggggcaa ccggcgcaat atcaacgtga
    36241 tcgcgcccga gcattagcga cacaatatga tgcgtagagg tgttttcgag catcacatcg
    36301 cccgccacct gaccatcgcg cataacggtg gcacaggagg caatgcggcg aatttcttcc
    36361 atccggtggc tgacataaat caccgccacg cccagtgccg acatcttttt caccgcgctg
    36421 atcaccagtt caacttccgc actggcaagc gagctggtgg gttcatcaag aatgaccacg
    36481 cgcggctcgc ccttcatcac ccgcgcaatt tccaccagtt gcttttgcgc cgggcttagc
    36541 gttgaaacaa gttgttcagg gctaacgtca acgcccagcg cctgtaagca acgttgggca
    36601 tcctgcgcca tttgcaggta atcaatcatg ccgttgcggc ggggccactg accgaggcag
    36661 aggttttccg ccactgtcag cccttccacc agacttaact cctgataaac cgcgcgtacc
    36721 cccagttcag cggcacggcg agtcagcgta gcttcgtcac cttccagtcg cgtctcgcca
    36781 atccagatat caccgctatc cggacgttcg ctaccggtaa gcattcgaat gagggtcgat
    36841 tttcccgcac cgtttttgcc taacagcgca cgaacttcgc cttttttgag cgtgaagtta
    36901 acgttatcca gcgcaacgac tccgggataa cgcttatttc ctgccaccac ttttgctacc
    36961 gggactgcct ctgttgccgt gaacataggg acctctgcga atcagcgatt agggcagacc
    37021 atcaacgtgc gttgccagcc actgtttacc gtcttccgtt ttggtataga gatcgattgg
    37081 cacctgaatc actttttcac cgtcggcttg tttattgata accttcaatg tttgcgtaaa
    37141 aacagcattg cccattttct taccagaaat atccaccacc gctttcagca cctggttgtt
    37201 ttccagctcc tgagcaattt cagtggtcat atcagaaccg aaaacagcaa tttttccggc
    37261 ctgattttga ttacgtaccg cttttaccgc gccgagtgtc gctccgcccg attcccccat
    37321 aatggcgttg agatccggcg tggagataat cagtttttca ccgacagaaa tcgctttatc
    37381 taaaacagtc ccttcctgat tagcgacaat ttgcgcgccg ggaacgcggg tttttaatac
    37441 ttcttcaaac cctttacgac gttgcacaca aacttcaaag gcttcgcaat tgatgacggc
    37501 aattttcggc tggtcaattt tgttggcaat aaaataatcg gcggcagcgt tacccagttt
    37561 tttaccaaat tccagcgggt caccaaccag atatgccgag acatatttat cgacaccctt
    37621 ttgattaata caggtgttgt agcaaatcac cggaataccc gcttcactgg cgcgacgaac
    37681 ggtacggcta ctgccatttt cagacactgc cgataaaata atggcatcga cattacgcgc
    37741 cacgagggta tcaacaaagg tactttcttt cgaaatatcg ccctgagcgt tagtttcaat
    37801 taactgtacc tgtactgaag aatcttttgc cgcatcctga acgccctgac gcactccggc
    37861 gtaatatccc tgggtatcaa ggtatattgc gccaatagtc atttctttat cagaagccct
    37921 ggcaaatagt gcgctaccca atagtgtagc cattaataat aaattacggg ttcttgtcat
    37981 ttttttaggc atagtgcctc ctgtagggtt tttatttaca acggcttatt ttaattattt
    38041 gagcgtgagc ggcagcacgt gccgctcaaa tattacagtt aatagggttt agtgaacgta
    38101 gctaaaaata aaaggcatca cggtggtgaa taatgccgct ttcggccagg ctggattaat
    38161 cgccatcaat tcagcgcgag cagattcata gtgcgcaacc tcccctaatc ccgcggccgc
    38221 cagcatcgcc accatcaggc atttttcttt atgctgctgt tgtaaatcgc catacagcga
    38281 caacaagtca ggctgcgaga cggcaaagaa atccgcctcg atactggttt tcgccatctc
    38341 ttgtgcccac tgtttcattt cactaaacag ttgctgtgca gtttgttgtt cgcccagcag
    38401 tcgcagcgcc attccttgcc agaagagata atcaaccggc tgatcgttgt aataactgtg
    38461 gatgttaatg gtgcgatcgc cggtcgccgc cagacgtaaa caacgcgtcg cttcagtttc
    38521 atcgccctgc gcgttggcgc atactgcctg ccagaaccag atgtcgttgt cagtttgccc
    38581 cggtaaacgg ccttcgctta aattctccgg ataatgcagc gcggcatgaa gcagttcgct
    38641 ggcctgctgc ggctctctgt catcaagatg ctgccaggcg cgtaataact ggttgaggat
    38701 aaactggctg gtgaccttcc cttccccacc ttcccacggg tggaatttgc gcgtggcgag
    38761 aatgtccgcc gctttgtctg cctgacccgt gagatgccac aaattgagca gttctgcggt
    38821 catgtcgtcg cgtttcagcg caatttccag attatcttcc agacgcgcca gtcgtttctc
    38881 cggtgtgaca ccgcttaact tatccagcaa atcccgttcg aaaagcagac gtgcatcctg
    38941 cggcgcaagc tgataagcat tatcaagata acgcgcggcc agctcataat cgtgctgctt
    39001 attccacgca tggatcgcca acccgcgcca gccgtcggca aactccggcg acatctctac
    39061 gcaacgctgc cataaggcaa tggctttgcc gtagctacgc ttgttgtagt agaagcaggc
    39121 cagtaaatgc cgggcaaacc agcactcttc gatactctcc agcgccgcca cttcttccag
    39181 cgtattcggg aaacggacaa actgcgggaa ggcatcaata gcttttgcga ccagttctcc
    39241 gcgttcagct tttggtagca aactggcttg caggtaaagc ggtagcgtgc gctgacagtc
    39301 cagtgcgttc agcatatctg ccgccagggc gggcatgccc cagttaatca gttgcccggc
    39361 ggtcatcagg gcgttaacgt cgcgtccctg acaaagacct cgccactgcg cgagggctga
    39421 ttcgctacga ccatcgaacc agttcagcca ccacagagtg gcgttcagcg gataatcacg
    39481 cagcagtttc tcgcgctgca aacgcgcgtt gtcctgacga ccacttaaca ccagtagcag
    39541 gttatgcagg caaagcactt cctgattggt tgggcaggcg cgaagacttt gttggcaaaa
    39601 atccagacca gcgtcgaagt taccattacg cgccgccagt cgtgccagac cataatagcc
    39661 gcccgcttta ctgttgccgc tccagaccgc acgccagaaa tcctcttcgg cttgttgata
    39721 ttgcccctga cgttcgtaag cactggcgcg aatcaaactc gcctgtccgc actgcggatt
    39781 tttgttcagc gcatgtgcgc gtttcagtgc ctgactggca tataccaccg cttgcgggaa
    39841 atctgcacgg ttatattcca gcattgccag cgccagatta cagcgataat ccagcggatc
    39901 cagcgccacg ccgcgcaggt agtaatcgaa cggtgaacgg ctggcgtgat gatattgctc
    39961 cagatgttga ccgataaacc aggcttcatc tgtactggta atgtcttgtg ctgccagtgg
    40021 cgctttggcg acgtccggca acggcaacgc ttgcggctga tgttcatgat aactcagtac
    40081 aatattgccg tcggcatcgg agagctcaat ggtcagcctt tcaggattga taccgtgcaa
    40141 cacgccctgg atggcggtcg ctggtgtcag ggccacggca tcatcgagca acgcgttgca
    40201 tttgccgatt tcgcggatcg ccaggcggta tccgttcaac ggagagatgg catacagccc
    40261 ccactcaatc ccccgctcac tacgctggag ttttatcacc gcatcgcggg aggcattttg
    40321 caccatgccc agagaatgat aaggcaggaa atactgctcg aaacgcttct cctcgtaagc
    40381 atcaagccag gtaaaatcag gctggttatc ggcaaaaata ccggtcatca gttcgatata
    40441 cgggccgtta ttgtcagtca ggctcttatc ccacgcctgg ccaaattcac tgtgtcccca
    40501 gctccactgt tttttacctg gcgcaatatg gtggttggca acgtgtagca aaccaccatc
    40561 ttcatcgtga caccacgcgc caacaaaatc gtactgtgat ttttcagcca tatatgaggt
    40621 tggaacgggc acatttttat agcgagaaat gtccactccg gcggagtagt ccactttgta
    40681 gtaagtgccg gtggcgatgg ggaaagcgga gacggcccgt ttgccgtgat caaacaccgc
    40741 cgttacatcc ggcgggaaga cgctctgatg cccttccccc cctttcactg ccgggttggc
    40801 ccaccacaag aaatgacgcg gcgtggcgtt gccgttatag acgcgactgg cgatttccag
    40861 cgccgcccgg tcagggcgca gggtgaaacc tgtcatcacc tgtaaaccgt gcatcgactc
    40921 cgtttcgccg acccacaccg tctgtgcgcc gtcgtcatgg gcttcgaggg tgaaatcaac
    40981 gggcataaag gtggtcgggc gatggtgttg cggccagtta aactcaatcc cgccggagat
    41041 ccacggcccc agcagtccca ccagcgccgg tttaatgact tcattgtgat aaacaaaatc
    41101 gcgctgtttc actttatccc acgcgcgatg cacccgaccg cccagttccg gcaggatcat
    41161 cactttgatg tagtcgtttt ccagccacac cgcctgccag gatttcaggg ttttctgctc
    41221 gctcagcgta tcggtcacgc cgtagggata aaccgcgccg gacgatccct gataaacgcg
    41281 attttccagg aacatgggat gtatatcctg cggcccggtt tcataggtcg ggatctcaac
    41341 gcgctcttgc cacactttta ctggagtcat ggtgccctca atattaacaa gacatactga
    41401 attaaaagat ttgtggcagt gtattgaaca atctggcaat gttttcgcgg aataatcacg
    41461 caattaacta aacaaggttt agtgaagatg agagcctgca ttaacaatca acagattcgc
    41521 caccataaca aatgcgtgat tctggaactg ctgtaccggc aaaagcgcgc caataaatca
    41581 acgctggccc ggctggcgca aatttcgatt ccggcggtca gtaatatttt gcaggaactg
    41641 gaaagcgaaa aacgggtggt gaatatcgac gatgaaagtc aaacgcgcgg acatagtagc
    41701 ggtacatggc tgattgcgcc ggaaggtgac tggacgctgt gtctgaacgt gacgcccacc
    41761 agcattgagt gtcaggtcgc taatgcctgt ttaagtccga aaggcgaatt tgagtattta
    41821 cagattgatg caccgacgcc gcaggcgctg ctgtccgaaa tcgaaaaatg ctggcatcgc
    41881 caccgtaaat tgtggccgga tcgcaccatc aatctggctc tggcaatcca cggtcaggtg
    41941 gatccggtga ccggcgtgtc gcaaaccatg ccgcaagcgc cgtgggcaac accgattgag
    42001 gtgaagtacc tgctggaaga gaagctgggt attcgggtaa tggtcgataa tgactgcgtg
    42061 atgctggcgc tggcggagaa atggcaaaat aattcgcaag tgcgtgattt ctgcgtaatc
    42121 aacgttgatt acggcattgg ctcgtcgttc gtgattaacg agcaaattta tcgcggcagt
    42181 ttgtatggca gtggacagat tggccacacc atcgttaatc cggatggcgt cgtctgcgac
    42241 tgcgggcgtt atggctgcct ggaaaccgtc gcctcgttaa gcgcattaaa aaaacaggcg
    42301 cgggtatggc taaaatcaca accggttaat actcagctta atcctgaaaa attgactacc
    42361 gcgcagttaa tcgctgcctg gcagagtgga gaaccgtggt tcaccagttg ggttgaccac
    42421 agtgccaacg ccattggttt gagtctgtat aacttcctca acatcctcaa tattaatcag
    42481 atttggttgt acggtcgcag ttgtgccttt ggtgagaact ggcttaatac cattattcgc
    42541 cagacgggat ttaacccgtt cgaccgcgac gaaggaccga gcgtgaaagc gacacagatt
    42601 ggctttgggc aattaagccg cgcacaacag gtactgggaa ttggctattt gtatgttgag
    42661 gcgcagttac ggcagatttg atggcgcgat aacgtagaaa ggcttcccga aggaagcctt
    42721 ttttcaacag attaaccgtt tcgcttatgc gtaaaccggg taacgtgcgc agatgtcgag
    42781 aactttacct ttgatgcgct cgataacggc ttcatcattg atgctgtcca gcacgtcaca
    42841 catccagcca gccagttctt tcgcttcggc ttctttaaag ccacgacggg taatcgccgg
    42901 agtacctaca cgaataccgg aagtcacaaa tgggctcttc ggatcgttcg gtacgctgtt
    42961 tttgttgacg gtgatgttag cacggcccag agcggcgtct gcttctttac cggtcaggtt
    43021 tttatcaacc agatcaacca ggaacaggtg gttatcagta ccgccggaaa ccactttgta
    43081 gccgcgttcg aggaacactt ctaccatcgc tttggcgttt ttcgcgacct gctgctggta
    43141 agttttgaac tcaggttcca tcgcttcttt cagagcaacc gctttaccgg cgattacgtg
    43201 catcaacgga ccgccctgac caccagggaa aacggcagag ttcagttttt tgtacagctc
    43261 ttcgctacca cctttcgcca ggatcaggcc gccgcgcgga cccgccaggg ttttgtgagt
    43321 ggtggtagtt actacgtgag catgaggaac cgggttcggg tagacgccag cagcaaccag
    43381 gcccgcaacg tgcgccatat caacgaacag gtaagcaccg atgctgtcag cgatttcacg
    43441 cattttcgcc cagtcaacca cgccggaata tgcagagaag ccaccgataa tcattttcgg
    43501 tttgtgttct ttagcttgtt tttccagatc ggcgtagtcg atatgaccag tagcatcgat
    43561 accgtaagga acgatgttgt acagtttacc ggagaagtta accggagaac cgtgagtcag
    43621 gtgaccgcca tgcgccagat tcatacccag aacggtatcg cctggttcca gcagcgcggt
    43681 gtagaccgca aagttagcct gggagccaga gtgcggctgg acgttagcgt agtcagcgcc
    43741 gaacagttct ttcgcacgat cgatcgccag ttgttcaacg atatcaacat actcgcaacc
    43801 gccgtagtag cgtttgcccg gataaccttc agcatatttg ttggtcagct gagaaccctg
    43861 cgcctgcatt acgcgcgggc tggtgtagtt ttcggaggcg atcagttcga tgtgctcttc
    43921 ctgacgtact ttttcctgct ccatagcctg ccacagttcg gcatcataat cggcaatgtt
    43981 catttcacgc tttaacatcc gcatctcctg actcagctaa caataaaatt tttggccttt
    44041 ataggcggtc ctgttggaca acggcgaaca gtataaccga atcattgtgc gataacaggt
    44101 cttgacaaag gaatttacgc aaacgattac cttcaggcta cgcaaggctt tggagaataa
    44161 agagcttgca accggaaacg gatttctttt caggtttgtg atgcaaattt ttcacttcat
    44221 cacattcttt ctgaaaaaca ccaaagaacc atttacattg cagggctatt ttttataaga
    44281 tgcatttgag atacatcaat taagatgcaa aaaaaaggaa gaccatatgc ttgacgctca
    44341 aaccatcgct acagtaaaag ccaccatccc tttactggtg gaaacgggac caaagttaac
    44401 cgcccatttc tacgaccgta tgtttactca taacccagaa ctcaaagaaa tttttaacat
    44461 gagtaaccag cgtaatggcg atcaacgtga agccctgttt aacgctgttg ccgcctacgc
    44521 cagcaatatt gaaaacctgc ctgcgctgct gccagcggta gaaaaaatcg cgcagaagca
    44581 caccagtttc cagatcaaac cggaacagta caacatcgtc ggtgaacacc tgttggcaac
    44641 gctggacgaa atgttcagcc cggggcagga agtgctggac gcgtggggta aagcctatgg
    44701 cgtactggct aatgtattta tcaatcgcga ggcggaaatc tataacgaaa acgccagcaa
    44761 agccggtggc tgggaaggta ctcgcgattt ccgcattgtg gctaaaacac cacgcagcgc
    44821 acttatcacc agcttcgaac tggagccggt cgacggtggc gcagtggcag aataccgtcc
    44881 ggggcaatat ctcggcgtct ggctgaagcc ggaaggtttc ccgcatcagg aaattcgtca
    44941 gtactctttg actcgcaaac cggatggcaa aggctatcgt attgcggtga aacgcgaaga
    45001 gggtgggcag gtatccaact ggctgcacaa tcacgccaat gttggcgatg tcgtgaaact
    45061 ggtcgctccg gcaggtgatt tctttatggc tgtcgcagat gacacaccag tgacgttaat
    45121 ctctgccggt gtcggtcaaa cgccaatgct ggcaatgctc gacacgctgg caaaagcagg
    45181 ccacacagca caagtgaact ggttccatgc ggcagaaaat ggcgatgttc acgcctttgc
    45241 cgatgaagtt aaggaactgg gtcaatcact gccacgcttt accgcacaca cctggtatcg
    45301 tcagccgagc gaagccgatc gcgctaaagg tcagtttgat agcgaaggtc tgatggattt
    45361 gagcaaactg gaaggtgcgt tcagcgatcc gacaatgcag ttctatctct gcggcccggt
    45421 tggcttcatg cagtttgccg cgaaacagtt agtggatttg ggcgtgaaac aggaaaacat
    45481 tcattacgaa tgctttggcc cgcataaggt gctgtgattt gatgttgccg gatggacaca
    45541 tccggcaacc cttgacgcgt tttaaattgc cgcgtcgtcc tcttcaccgg tacggatgcg
    45601 aatgacccgt gccacgtcga agacgaagat tttaccatca ccgattttgc cggtttgcgc
    45661 cgtgcgaata atggtatcga cacaggtatc gacaatgtcg tccggtacga caatctcaat
    45721 tttcactttc ggcagaaaat ccaccatata ctccgcacca cggtacagct cggtatggcc
    45781 tttctggcgg ccaaaacctt tcacctcagt gaccgtcatg ccggtaatac cgacttcagc
    45841 cagtgcttcg cggacatcgt ccagcttgaa gggtttaata atcgcatcaa tctttttcat
    45901 gctattcctt gaaaaggtcg cctgtctttt gatctgctaa acgtaacaca taacgccaat
    45961 tcattccttg aaatcgtttg catccagctc gtgtcgggaa agcagtttat aaaattctgt
    46021 ccggttgcgc cccgccattc tcgccgcgtg ggtgacgttg cctttggtga tttgcagcag
    46081 cttacgcaaa tagttgagtt caaactgatt acgtgcctca acaaaggttg gcagcgccgt
    46141 attttcaccc tccagcgcct gctccaccag cgcatcacta attaccggag atgaggtcag
    46201 cgccacacac tgttcaatca cgttgaccaa ctggcgcacg ttgcccggcc agcttgcagt
    46261 catcaggcgt ttcattgcat cggtggagaa cgcgcggaca aacggtttat gtcgctctgc
    46321 cgcctggcgc aacaggtgat ttgccagtag cggaatgtct tctgtgcgct ccgccagtgc
    46381 cggaattttc aggctgacaa cattgaggcg gtaataaaga tcttcacgga attccccacg
    46441 cgccatcgct tttggcaggt cgcggtgagt ggcggaaata atccgcacgt caatatcaat
    46501 atcgcggtta ctgcccagcg ggcgcacttt acgctcctgc aacacgcgca gcagtttgac
    46561 ctgcaacggt gcaggcatat cgccaatctc atcaagaaat agcgtaccgc cttccgccgc
    46621 ctggaataaa ccttcgcgat tgctgacagc gccagtaaac gcgccacgtg catgaccaaa
    46681 cagctccgac tccagcaatt gctcgggtaa tgcgccacag ttaatagcaa taaatggctt
    46741 gctgttgcgc gggctggcgt tgtggatagc ctgggcgaaa atctctttcc cggtgccgct
    46801 ctggccgtta atcaaaacgc taacgtctga ttgtgccacc agccgcgcct gttccagcaa
    46861 gcgcagcatc agcgggctgc gggtgacaat cgcctcgcgc caacgttcgt cggtggctgg
    46921 cgcggattgt tccagcgcat cgtcaattgc ctgatatagc gcatctttat cgacaggctt
    46981 ggtgagaaaa ctgaacacgc cctgctgtgt tgcagcaacg gcatcaggaa tagaaccatg
    47041 cgcggtaaga ataattactg gcattcccgg ctgcactttc tggatttcag caaacagctg
    47101 cataccgtcc atttcatcca tccgcaggtc gctgatgact aaatctactt tttcgcgatt
    47161 cagtacccgc aatccttcag caccactttc cgccgtgacc acactgtagc cttcgctggt
    47221 cagacgcagg ccaagcagtt tcagcaatcc cggatcgtca tcgaccagta ataaatgcgc
    47281 aggtttatgg ctcatcagga gtgacctcat cggtggatgg cgcgggcttt tcactctcgt
    47341 gcggcgtatc cggcgagaaa tttccagccg gtttgcgggt cgagagctgg cgttcaatat
    47401 cggtcaggtt ttccagcttg cgagtagtga gttccagttg ctgttgtaga acgtgatgtt
    47461 gctggcgcaa agtatccagc tcgctgtcgc tggactgctg gagtttgctg tagcgttggc
    47521 gctcttccgc cagttgcagt tgcagcgcct gaccgtcgcg ccaaagttga tacagtgggc
    47581 gaacctgtgc cgggatctcg gtacttagcg catcaatgcg cgcgaccagc tggcggcgct
    47641 catacggcgt aattttggcg tcggcgagca aaatcccttg tttaaaggta ttttgccagt
    47701 tgccgtcgtc atattggcgg gcttgctgac gcgactgcgc aggcattaaa cgatcagcac
    47761 aatccatcgc ccgcagccag taaagcggat tggtttcggt tgatttacct tgcagcgccc
    47821 agatgtcgct acattcagta gaaagatagt cagccagttg ataaaccgga attttttctt
    47881 ctgctggcgt atcaatggct ggcctattgt gactctgcac gcaacccagc aatgccagac
    47941 atggcagccc tgccagccac agccgtcggg gcaataatcg ttgaaaaatg tgtcgcatat
    48001 tcaccagact taaagcctat cccagtgggc gtaattgttg caggcagttt ggacatggac
    48061 agcgcggaga aaccggagcg tacatgtcgt acgtgaagat ttcgagcact gcccggggcc
    48121 gaactgacaa ataaaatagc ctgatgggat gggctgttag cattatttcg tgtttttcga
    48181 cgacggtaat tcaatgcgga aacaaacgtc ttgcccgctc tcgtcgacca gatacagttc
    48241 cccttgcata cggcgaatac aatccctggc aatgcttaat cccagaccgc tgcccttcac
    48301 cgcccctttt cgctggtggc ttccctgaaa aaagggttcg aagatcatgg cgcgttcctc
    48361 ttgcggaatg ggcgtgcctg tattgatgac atcaatataa acccgcgcac catgtaaact
    48421 gctgcgaagg caaatgttac cggattcagc cccgtagtgc accgcattgg agtaaagatt
    48481 atccagtacg ctcatcagca gcattggctc cgccaggcaa gctgttgctt tgagatcaac
    48541 gtcggtatgc atcattttag cccgtgcggg caggctatga gcagaaacca atgtctccac
    48601 cagcggtgct aactcaacat tctccagttc caccgcactg tccgcctgtt tacggttgta
    48661 atcaagcagt tgttcgatca gtttttgcaa attgcggctg ctgctatcaa gaatgctcac
    48721 cacctctttt tgctctggcg taagcggccc gacaacctga tcagccagta attcagtgcc
    48781 ctcgcgcata ctcgccagtg gcgtttttaa ttcatgagat aaatgtctta aaaattgatg
    48841 gcgttgggat tccagccatg acaggcgctc acttaaccaa agaatacgtt gcccaaccga
    48901 gcgtaactcg ctcggtccac tgaacgagac gctattgccc agagaacgcc cttcccccag
    48961 ccggttgatc atgcgctcga tatttttcac cggaccgata atcatcctcg tgaaaagcag
    49021 taccatcacc agactcacca gaaatagcac cagcgattgc caaccaaaat attgcccacg
    49081 ttcggcgatt tcacgctgaa gttgctgccc acgagagaac accactgtgc gcgtggcctg
    49141 caccatttcg gtattggcac tggcaaaggc ttccagacgc gcggcggcag cagcatcggg
    49201 accgctgttg ttacactgaa gttgagccag attgttcaaa tcctgacgta atgcctggta
    49261 gagtttatcg tccggcagca cgcctgcgtg ggcatcgagc atttcgctgt aacgcttgcg
    49321 ctggctttga taaaccttcg caagcgtagg gtcgtccagc acgcagtact gacggtagct
    49381 gcgctccatc tccagtgccg cgttggtcat cgcttcactg cgccgggcat caataagcgt
    49441 agtgcggtta accagcgccg cctgatcgct aagtgcattc aggctttgcc aggcttgcca
    49501 tgccagcacc aacaggggca gcagaatcag caaaaatgcc agcattacca gttgtcgtaa
    49561 tgagcgggga aaaacgggcc agcgtttcaa ggcgttactc tcgtcagacg cgaatagcct
    49621 gatgctaacc gaggggaagt tcagatacaa caaagccggg aattacccgg ctttgttatg
    49681 gaataaggcg gtgcctaact cgacgtttcg cccgatggtt gatatagcta agctgatatc
    49741 agaagttgga cggcaggcac cttgttgtgc gtcattcgaa ttttatgtag cacgtcccga
    49801 aggggctgac ataagtcggt gaatgagcca ctgcttacta ttatgcagta actgtgccaa
    49861 taaagaaaat agtttggtaa catactgatt atacgctgct ttgaggggtt tatggcttct
    49921 ccgctatttg aatgtaaatc agccactgtg tcgctaaaaa gagacaactt aagataaact
    49981 tattagataa tattaaaatc aatgagttaa gtgtcaccaa aaagcgacac ggcaaaccac
    50041 ccattgtcgt gatttaaaga cacaaaaaag ctcccgaagt tgggagctca taatagcggt
    50101 tggtgcttaa tgccggatgc ggcgtgaatg cctcatccgg gctgcaatac caatgggttg
    50161 acgacttacc ccaactgctt acgtgcattg cggaaaatgc gcatccatgg gccatcctcg
    50221 ccccagtttt ccggatgcca ggagttgctg acagtacgga aaactcgttc cgggtgcggc
    50281 atcataatgg tgactcgacc actttcagtc gtgaccgccg taataccgtt cggtgaaccg
    50341 ttcgggttag ccgggtaggt ttcagtgact ttgccgaagt tatcgacata gcgcagtgcc
    50401 accagccctt tgctttccag tgccgccaga tgcgccgcat cacgcacttc cacgcgccct
    50461 tcaccatgag acacagcaat cggcatctgc gagcccacca tcccctgcaa cagcagcgac
    50521 gggctttggg ttacttctac cagactgaaa cgcgcttcaa agcgatcgga ggtgttacgc
    50581 acaaaacgtg gccacaactc gctacccggg atcagttcac gcagattaga catcatctgg
    50641 caaccgttac atacccccag cgccagcgtt tgcggacgat ggaagaaggt tgcaaactca
    50701 tcgcgtacac ggtcattgaa caggatagac ttcgcccaac cttcaccagc gcccagcaca
    50761 tcaccgtagg agaaaccacc acacgcgacc agggcgtgga aaccctccag gcccgtgcgt
    50821 ccagccagca gatcgctcat atgcacgtcg atagcatcga agcctgcacg gtggaaagct
    50881 gccgccattt caacatgcga gttcacgccc tgctcacgca gcacggcaac tttcgggcgc
    50941 acgccagtgg caataaacgg tgctgccaca tcttcgttga tatcaaacga cagtttcaca
    51001 ttcaggcccg gatcggcgtc gtttgatttc gcctggtgtt cctgatcggc acactccggg
    51061 ttgtcacgca ggcgctgcat ctgccaggta gtttctgccc accagacacg cagcgtggtg
    51121 cggctttcgc taaatacagt ctgcccgttg gcagtaatca caaaacggtc accggaaacc
    51181 gcctgcccaa cataatggac gcaatcagca agcccatgct gtgccagtac cgcttcaacg
    51241 gcttcacgat cagcagctcg aacctgaatc accgcaccca gttcttcgtt aaacaacgcc
    51301 gccaggcgat cgtcacccag agaggcgata tccgcgtcaa tgccacaatg accagcaaag
    51361 gccatttccg ccagcgttac cagcaggccg ccatcagagc ggtcgtgata cgccagcagc
    51421 ttacgctgtg caaccagcgc ctgaatcgcg tcatagaagc ctttcagttg ggcaacatcg
    51481 cgtacatctg ccggtttgtc gccaagctga cgataaacct gcgccagtgc cgttgcgccc
    51541 agcgcgttat tgcctttgcc caaatcaatc agcaacagtg cgttatcttc ggtagaaagc
    51601 tgcggcgtaa tggtgtgacg tacatcttcc acgcgggcaa atgcggaaat caccagcgac
    51661 aacggcgacg tcatttcgcg ctcttcgtta ccttcctgcc agcgggtttt catcgacata
    51721 gagtctttac ccaccggtat cgtcaggccc agcgccggac aaagttcttc gcccacagct
    51781 ttaacggctt catacaggcc cgcatcttca ccagggtggc ctgccgccgc catccagttg
    51841 gcggaaagtt taatgcgttt gatatcgcca atttgtgttg cggcgatgtt ggttagcgct
    51901 tcaccgactg ccagacgggc agaagcggcg aaatccagca gcgcaactgg cgcacgttcg
    51961 ccaattgcca tcgcttcgcc atagtagctg tcgaggctgg cggtagtgac cgcgcagtta
    52021 gcgaccggca cctgccacgg ccccaccatc tgatcgcgcg ccaccatgcc ggttacgctg
    52081 cggtcaccga tggtcaccag gaaagttttc tccgccacgg tcggcagatg cagcacgcgt
    52141 ttcaccgcgt cagcgatggt gatcccttca cgggccagcg cgtcgccttt cgctttcagc
    52201 gtttgcacat cgcgggtcat cttcggcgtt ttgccaagca gtacgtccag cggtagatca
    52261 atcggctgat tatcaaaatg acgatcgtgc agagaaagat gcagttcttc ggtcgcttca
    52321 ccaatcaccg cgtacggtgc gcgttcacgc ttacacagtt cgtcaaacag cggtaactga
    52381 tcggcagcaa ctgccagcac gtagcgttcc tgggattcgt tacaccagat ttccagcggg
    52441 ctcatgcctg gctcgtcgct cagaatatcg cgcagttcga atttaccgcc gcgcccgccg
    52501 tcgctcacca gttccggcat ggcgttagaa agaccgcctg cgccaacgtc gtggataaac
    52561 aggattgggt tggcatcacc aagctgccag caacggtcga tcacttcctg gcagcgacgc
    52621 tccatctccg ggttgtcgcg ctgtacggaa gcaaagtcga ggtcggcatc agactgacca
    52681 gatgccatgg aagacgccgc gccgccgcca agaccgatgt tcattgccgg gccgccgaga
    52741 acgaccagct tcgcaccaac gttgatctcg tctttttgta cgtgatcggc gcgaatgttg
    52801 ccgatcccac ccgccagcat gatcggtttg tgataaccgc gcagctcttc gccattgtgg
    52861 ctgttcactt tttcttcata agtacggaag tagccgttca gtgccgggcg accaaattcg
    52921 ttgttaaacg ccgcgccgcc cagcgggcct tcggtcatga tgtccagcgc ggtgacaatg
    52981 cgctcaggct taccgaaatc ttcttcccac ggctgttcaa agcccggaat acgcaagttg
    53041 gatacggaga aaccaaccag accggctttc ggctttgcgc cgcgcccggt ggcaccttca
    53101 tcgcggattt caccgccaga accggtcgcc gcacccggcc acggagaaat cgccgtcggg
    53161 tggttgtggg tttcaacttt catcagaata tgcgccggtt cctgatggaa atcgtagcgg
    53221 cccgtttcgt ggtcagcaaa gtagcgaccc acttcagaac cttccattac agcggcgtta
    53281 tctttataag cagagagaac gtggtctggc gtggtttcga aggtgttttt gatcatcttg
    53341 aacagcgatt tcggctgctg ttcaccgtcg ataatccagt cggcgttaaa aattttgtga
    53401 cggcagtgct cggagttcgc ctgcgcgaac atatacagct cgatgtcgtt cgggttacga
    53461 cccagctttg tgaacgcatc ctgcagatag tcaatttcat cttccgccag tgccaggcca
    53521 agacgcaggt tagcgtcgat tagcgcctga cggccctgcc ccagcaaatc tacgctggtg
    53581 accggagtcg gttgatggtg tgcaaacaac tgctctgcat catctaaagc aaaaaagacc
    53641 gtttccatca tgcggtcgtg cagttcagcg gtcacctgct gccattgttc attggtcagc
    53701 gtaccggctt ctatatagta agccatgccg cgctcaaggc ggtttacctg ttgtagaccg
    53761 caattatggg cgatatcggt cgctttcgaa gaccagggag agatggtgcc aggacgcggg
    53821 gtcaccagca ggagtttgcc ttgcggggcg tggctggcga gtgccggggc atatttcagc
    53881 aggcgttcaa gttgtgcgtg ctcatcatcg tttaacggcg cattgaggtc agcaaaatgg
    53941 acatactcgg cgtaaatagt gtgaaccggg agcctggcag cctgaaaacg tgccagcagt
    54001 ttgttgattc ggaatgccga caatgcaggc gaaccacgca gaatttccat cataagtctc
    54061 tcgtcttcta agctttcggt gtacccaagg gggggaaacg ggcgtcatta taaagaatct
    54121 gatgcgctga cgaaaccgtt tgcgtggaaa taaaatcacc atcgtgaatt agcaacgcgt
    54181 gccgccaatg gctgtaataa gttgccatct ggcgcaggtt tacgcaaaat gccgctcatt
    54241 tatgagtaaa cctttcacta ttattacgtt ttttcaagct gggacgcgca cgacacagag
    54301 aattaactaa ttgaaaaaat taaagattaa ttatctgttc atcggcattc tggcactgct
    54361 gctcgcggtc gctctctggc catccattcc ctggtttggt aaagccgaca accgtatcgc
    54421 cgccattcaa gcgcggggag agttgcgtgt gagcaccatt catactcccc tgacttataa
    54481 cgaaatcaac gggaaacctt ttggcctgga ttacgaactg gcgaaacagt ttgccgatta
    54541 cctcggtgta aaactgaaag tgaccgtgcg gcagaatatc agccagctgt ttgacgacct
    54601 cgataatggt aacgccgacc tgctggcggc aggactggtc tataacagtg agcgggtaaa
    54661 aaattatcag cctggtccta cctattattc cgtgtcacaa caactggttt ataaagtggg
    54721 tcagtatcgc ccacgtacgc tgggcaacct gacggcggag caactcaccg ttgcaccggg
    54781 tcacgtggtg gttaacgatc tccagaccct gaaagacaca aaattcccgg aattaagctg
    54841 gaaggtagac gacaaaaaag gctctgcgga attaatggaa gatgtcatcg aaggaaaact
    54901 cgattacacc attgctgatt ctgtcgccat cagcctgttt cagcgcgttc acccggaact
    54961 cgccgtagcg ctcgatatca ccgatgaaca accggtgacc tggtttagcc cgttagatgg
    55021 cgataatacc ctttccgccg ccctgctcga cttcttcaac gaaatgaatg aagacggtac
    55081 actggcacgc attgaagaga aatacctggg gcatggcgat gattttgatt acgtcgatac
    55141 gcgcacattt ttacgcgccg tcgatgcggt actgccgcag ttaaagcccc tgtttgagaa
    55201 atacgccgaa gaaattgact ggcgtttgct ggccgctatt gcttatcagg aatcgcactg
    55261 ggatgcgcag gccacttcac cgacgggtgt gcgcggcatg atgatgttaa ccaaaaacac
    55321 cgcgcaaagc ctcggcatta cggatcgtac cgatgccgaa cagagcatca gcggcggcgt
    55381 gcgttattta caggatatga tgagtaaagt gccggaaagt gtgccggaga acgaacggat
    55441 ctggtttgcc ctcgccgcgt acaatatggg ctatgcgcat atgctggatg cccgcgccct
    55501 gacggcaaaa accaaaggta atcctgacag ttgggctgac gtaaaacagc gtctgccttt
    55561 acttagccag aaaccctatt acagcaagct gacttacggc tacgctcgtg ggcatgaagc
    55621 ctacgcttat gtcgaaaata ttcgtaaata ccagattagc ctggtgggtt atctgcagga
    55681 gaaagagaag caggctacag aagcggcgat gcaactggcg caggattatc cggcggtatc
    55741 gcctacggag ctgggcaaag agaaatttcc ttttctctcg tttctttccc agtcgtcatc
    55801 aaactatttg acccactctc cctctctgct gttttccaga aaagggagtg aagagaaaca
    55861 aaattaatct gtcgaggatt gcgctttttt ctgcgcttta atttcctggc ggcgcatgcg
    55921 aaagaagtca ctgagcaacg ccgcacactc atccgccagt attccttccg taatttccac
    55981 ccggtgattc atacccggat gatgcagcac atccattaaa gatcccgcag cgccagtttt
    56041 cgcgtcacgc gcaccaaaga ccacgcgacc aatgcgactg tggatcattg ctccggcaca
    56101 cattacacag ggttcaagcg tgacatacaa cgtggcgtcg atcagacgat aattttgcat
    56161 caccagaccg ccctgccgta gggccatgat ttctgcatgt gcggtgggat catgtcgacc
    56221 aatcggacgg ttccagcctt cgccaataac acgattgtta tgcactaata ccgcgccgac
    56281 cggtacttcc cgctcatccc aggcacgctt cgccagcgtc attgcatgac gcatccagta
    56341 ttcgtagcta aattcgactt cagacaaaaa gaaaactccg gttataaaag cgcggcgcat
    56401 tatacacgga cgctatgctt tactctattc cagttgctgg agttcaccgc gcggggttac
    56461 ccgccagcga tgctggcaaa agtaaagcag cgggttgtcc tggttactgt cgctatagcc
    56521 actgtacagc cgcagcggcg tgccgatttt gcgctccagt tgcgcgacct tctcatgccc
    56581 gaggcaacgc atcgtcaata cccaaccacc atagccacgc tgaatctgac tggcgataag
    56641 attaacccgc ggcagccagg gcgtatcgaa ataaaccgct tcaaccagcg gctgcggaga
    56701 gccggtaatc aaccagatat cagcatcgga acttaacagg taggtggtta atcgctcctg
    56761 aaccagcgga aaggcggtaa cattgtcacg aaaccagcgt acgaaatcgg cctgcaacgc
    56821 ctgcaaacgc gcttcattgt gaccaaaagt gcatccccac agaagcagac tcatcggcca
    56881 gcgtgccgca cgacctttta tcaataacgc aatggctatg atcggcaaca acggcagaac
    56941 aagtaacgca ttcagcggtt ggcgacgcag taaataacgc aaaaaactgc cgaacatatc
    57001 ctgctgatgc aatgttccat ctaagtcaaa aaacaccaca cgacgctcgt gagttgccaa
    57061 accatactcc tctggactga aacatcctga ttaattttcg ctactacata gcctaacaga
    57121 tagatcatca cttttccggc aacaatcgac cgcatcatgg ctaactggaa tttttaattc
    57181 atggcaatta gcggcaatgg aatataaaat tcactcgcgt gtgtctcata ttgcctgttg
    57241 tcgccattat ttcatcgcga ggaaagatat gaacggctta cttcgtatcc gtcagcgtta
    57301 ccaggggctt gcccagagtg ataaaaaact ggcggattat ctgctgctac aacctgatac
    57361 ggcgcgccat ttaagctctc agcaactggc caacgaagcc ggagtcagtc agtccagcgt
    57421 cgtgaagttc gcgcaaaaac tcggctataa aggttttccg gcactaaagt tggcgttgag
    57481 tgaagcgctg gcaagccagc cggaatctcc ctccgtgccc attcataacc aaatccgcgg
    57541 tgatgatccg ttacggctgg tcggcgaaaa actgattaaa gaaaatactg ccgctatgta
    57601 cgcaacgtta aacgttaata gtgaagagaa actgcatgaa tgcgtggcaa tgttgcgctc
    57661 tgcgcggcgg ataattctga ccggtattgg cgcttcgggt ctggtggcgc aaaactttgc
    57721 ctggaagctg atgaagattg gcttcaatgc tgccgcagtg cgcgatatgc atgcgctact
    57781 cgctaccgta caggcgtcgt cccctgacga tctgttatta gccatttcct acaccggtgt
    57841 acggcgcgag ttaaacctgg cggcagatga gatgctgcga gtgggcggaa aagtgctggc
    57901 gattaccggc tttactccca atgccctgca acagcgtgct tctcattgcc tgtataccat
    57961 tgccgaagaa caagcgacaa acagtgcttc aatctctgct tgtcacgcgc agggaatgtt
    58021 aacggatttg ctgttcattg cgctgattca gcaggatctg gaactggcac cagaacgtat
    58081 tcgtcatagc gaagcgctgg tgaaaaaact ggtctgagta aagaatgcgc gtataatgcc
    58141 cgcccggttt gtgttgtttt gagagtttcc ttatggcgct gttaatcact aaaaaatgca
    58201 tcaattgtga tatgtgtgaa cccgaatgcc cgaatgaggc gatttcgatg ggtgaacata
    58261 tctacgagat taacagcgat aagtgtaccg aatgcgtagg gcactacgag acaccaacct
    58321 gccagaaggt gtgtccgctc cccaatacta ttgtgaaaga tccgcaacat gtcgagacgg
    58381 aagaacagtt gtggga
//
LOCUS       DAHHTG010000011        57223 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.011, whole genome shotgun sequence.
ACCESSION   DAHHTG010000011 DAHHTG010000000
VERSION     DAHHTG010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 57223)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 57223)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..57223
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.011"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            <1..531
                     /locus_tag="NBI74_000695"
     CDS             <1..531
                     /locus_tag="NBI74_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001683231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HCD1931071.1"
                     /translation="YYSGYGHTKLVAEKVAQGAHADLISIDNEGNITEQEWEKLDAAD
                     AIIFGAPTYMGGAPWQFKKFADASSKAWFTRKWQDKVFGGFTNSASLNGDKQVTLIYL
                     QTLASQHGGLWVSLGQAPANVLASTREDVNNLGGSVGLLVQSPADAGAEQIPTGDLDT
                     AVKYGERVATITARLK"
     gene            complement(702..1694)
                     /gene="rpoS"
                     /locus_tag="NBI74_000696"
     CDS             complement(702..1694)
                     /gene="rpoS"
                     /locus_tag="NBI74_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000081498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoS"
                     /protein_id="HCD1931072.1"
                     /translation="MSQNTLKVHDLNEDAEFDENGVEVFDEKALVEEEPSDNDLAEEE
                     LLSQGATQRVLDATQLYLGEIGYSPLLTAEEEVYFARRALRGDVASRRRMIESNLRLV
                     VKIARRYGNRGLALLDLIEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERAIMN
                     QTRTIRLPIHIVKELNVYLRTARELSHKLDHEPSAEEIAEQLDKPVDDVSRMLRLNER
                     ITSVDTPLGGDSEKALLDILADEKENGPEDTTQDDDMKQSIVKWLFELNAKQREVLAR
                     RFGLLGYEAATLEDVGREIGLTRERVRQIQVEGLRRLREILQTQGLNIEALFRE"
     gene            complement(1757..2896)
                     /gene="nlpD"
                     /locus_tag="NBI74_000697"
     CDS             complement(1757..2896)
                     /gene="nlpD"
                     /locus_tag="NBI74_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator NlpD"
                     /protein_id="HCD1931073.1"
                     /translation="MSAGSPKFTVRRIAALSLVSLWLAGCSDTSNPPAPVSSVNGNAP
                     ANTNSGMLITPPPKMGTTSTAQQPQIQPVQQPQIQATQQPQIQPVQPVAQQPVQMENG
                     RIVYNRQYGNIPKGSYSGSTYTVKKGDTLFYIAWITGNDFRDLAQRNNIQAPYALNVG
                     QTLQVGNASGTPITGGNAITQADAAEQGVVIKPAQNSTVAVASQPTITYSESSGEQSA
                     NKMLPNNKPTATTVTVPVTVPTASTTEPTVSSTSTSTPISTWRWPTEGKVIETFGASE
                     GGNKGIDIAGSKGQAIIATADGRVVYAGNALRGYGNLIIIKHNDDYLSAYAHNDTMLV
                     REQQEVKAGQKIATMGSTGTSSTRLHFEIRYKGKSVNPLRYLPQR"
     gene            complement(3036..3662)
                     /gene="pcm"
                     /locus_tag="NBI74_000698"
     CDS             complement(3036..3662)
                     /gene="pcm"
                     /locus_tag="NBI74_000698"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HCD1931074.1"
                     /translation="MVSRRVQALLDQLRAQGIQDEQVLNALAAVPREKFVDEAFEQKA
                     WDNIALPIGQGQTISQPYMVARMTELLELTPQSRVLEIGTGSGYQTAILAHLVQHVCS
                     VERIKGLQWQARRRLKNLDLHNVSTRHGDGWQGWQARAPFDAIIVTAAPPEIPTALMT
                     QLDEGGILVLPVGEEHQYLKRVRRRGGEFIIDTVEAVRFVPLVKGELA"
     gene            complement(3656..4417)
                     /gene="surE"
                     /locus_tag="NBI74_000699"
     CDS             complement(3656..4417)
                     /gene="surE"
                     /locus_tag="NBI74_000699"
                     /EC_number="3.1.3.5"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HCD1931075.1"
                     /translation="MRILLSNDDGVHAPGIQTLAKALREFADVQVVAPDRNRSGASNS
                     LTLESSLRTFTFENGDIAVQMGTPTDCVYLGVNALMRPRPDIVVSGINAGPNLGDDVI
                     YSGTVAAAMEGRHLGFPALAVSLDGHKHYDTAAAVTCSILRALCKEPLRTGRILNINV
                     PDLPLDQIKGIRVTRCGTRHPADQVIPQQDPRGNTLYWIGPPGGKCDAGPGTDFAAVD
                     EGYVSITPLHVDLTAHSAQDVVSDWLNSVGVGTQW"
     gene            complement(4398..5447)
                     /gene="truD"
                     /locus_tag="NBI74_000700"
     CDS             complement(4398..5447)
                     /gene="truD"
                     /locus_tag="NBI74_000700"
                     /EC_number="5.4.99.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(13) synthase TruD"
                     /protein_id="HCD1931076.1"
                     /translation="MIEFDNLTYLHGKPQGTGLLKANPEDFVVVEDLGFEPDGEGEHI
                     LVRILKNGCNTRFVADALAKFLKIHAREVSFAGQKDKHAVTEQWLCARVPGKEMPDLS
                     AFQLEGCQVLEYARHKRKLRLGALKGNAFTLVLREVSNRDDVEQRLIDICVKGVPNYF
                     GAQRFGIGGSNLQGALRWAQTNTPVRDRNKRSFWLSAARSALFNQIVAERLKKADVNQ
                     VVDGDALQLAGRGSWFVATTEELAELQRRVNDKELMITAALLGSGEWGTQREALAFEQ
                     AAVAEETELQTLLVREKVEAARRAMLLYPQQLSWNWWDDVTVEIRFWLPAGSFATSVV
                     RELINTTGDYAHIAE"
     gene            complement(5444..5923)
                     /gene="ispF"
                     /locus_tag="NBI74_000701"
     CDS             complement(5444..5923)
                     /gene="ispF"
                     /locus_tag="NBI74_000701"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HCD1931077.1"
                     /translation="MRIGHGFDVHAFGGEGPIIIGGVRIPYEKGLLAHSDGDVALHAL
                     TDALLGAAALGDIGKLFPDTDPAFKGADSRELLREAWRRIQAKGYALGNVDVTIIAQA
                     PKMLPHIPQMRVFIAEDLGCHMDDVNVKATTTEKLGFTGRGEGIACEAVALLIKATK"
     gene            complement(5923..6633)
                     /gene="ispD"
                     /locus_tag="NBI74_000702"
     CDS             complement(5923..6633)
                     /gene="ispD"
                     /locus_tag="NBI74_000702"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HCD1931078.1"
                     /translation="MATTHLDVCAVVPAAGFGRRMQTECPKQYLSIGNQTILEHSVHA
                     LLAHPRVKRVVIAISPGDSRFAQLPLANHPRITVVDGGEERADSVLAGLKAAGDAQWV
                     LVHDAARPCLHQDDLARLLALSETSRTGGILAAPVRDTMKRAEPGKNAIAHTVDRNGL
                     WHALTPQFFPRELLHDCLTRALNEGATITDEASALEYCGFHPQLVEGRADNIKVTRPE
                     DLALAEFYLTRTIHQENT"
     gene            complement(6652..6963)
                     /gene="ftsB"
                     /locus_tag="NBI74_000703"
     CDS             complement(6652..6963)
                     /gene="ftsB"
                     /locus_tag="NBI74_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsB"
                     /protein_id="HCD1931079.1"
                     /translation="MGKLTLLLLAILVWLQYSLWFGKNGIHDYTRVNNDVAAQQATNA
                     KLKARNDQLFAEIDDLNGGQEALEERARNELSMTRPGETFYRLVPDASKRAQSAGQNN
                     R"
     gene            complement(7156..7479)
                     /locus_tag="NBI74_000704"
     CDS             complement(7156..7479)
                     /locus_tag="NBI74_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3561 family protein"
                     /protein_id="HCD1931080.1"
                     /translation="MRNSHNITLTNNDSLTEVEDTTWSLPGAVVGFLSWLCALAIPML
                     IYGSNTLLFFIYTWPFFLALMPVAVVVGIALHSLMDGKLRYSIVFTLATVGIMFGALF
                     MWLLG"
     gene            complement(7528..8133)
                     /gene="cysC"
                     /locus_tag="NBI74_000705"
     CDS             complement(7528..8133)
                     /gene="cysC"
                     /locus_tag="NBI74_000705"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HCD1931081.1"
                     /translation="MALHDENVVWHSHPVTLQQREQHHGHRGVVLWFTGLSGSGKSTV
                     AGALEEALHKLGVSTYLLDGDNVRHGLCSDLGFSDADRKENIRRVGEVANLMVEAGLV
                     VLTAFISPHRAERQMVRERVGEGRFIEVFVDTPLAICEARDPKGLYKKARAGELRNFT
                     GIDSVYEAPESAEIHLNGEQLVTNLVQQLLDLLRQNDIIRS"
     gene            complement(8133..9560)
                     /gene="cysN"
                     /locus_tag="NBI74_000706"
     CDS             complement(8133..9560)
                     /gene="cysN"
                     /locus_tag="NBI74_000706"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysN"
                     /protein_id="HCD1931082.1"
                     /translation="MNTALAQQIANEGGVEAWMIAQQHKSLLRFLTCGSVDDGKSTLI
                     GRLLHDTRQIYEDQLSSLHNDSKRHGTQGEKLDLALLVDGLQAEREQGITIDVAYRYF
                     STEKRKFIIADTPGHEQYTRNMATGASTCELAILLIDARKGVLDQTRRHSFISTLLGI
                     KHLVVAINKMDLVDYSEETFTRIREDYLTFAGQLPGNLDIRFVPLSALEGDNVASQSE
                     SMPWYSGPTLLEVLETVEIQRVVDAQPMRFPVQYVNRPNLDFRGYAGTLASGRVEVGQ
                     RVKVLPSGVESNVARIVTFDGDREEAFAGEAITLVLTDEIDISRGDLLLAADEVLPAV
                     QSASVDVVWMAEQPLSPGQSYDIKIAGKKTRARVDGIRYQVDINNLTQREVENLPLNG
                     IGLVDLTFDEPLVLDRYQQNPVTGGLIFIDRLSNVTVGAGMVHEPVSQATAAPSEFSA
                     FELELNALVRRHFPHWGARDLLGDK"
     gene            complement(9562..10470)
                     /gene="cysD"
                     /locus_tag="NBI74_000707"
     CDS             complement(9562..10470)
                     /gene="cysD"
                     /locus_tag="NBI74_000707"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HCD1931083.1"
                     /translation="MDQKRLTHLRQLEAESIHIIREVAAEFSNPVMLYSIGKDSSVML
                     HLARKAFYPGTLPFPLLHVDTGWKFREMYEFRDRTAKAYGCELLVHKNPEGVAMGINP
                     FVHGSAKHTDIMKTEGLKQALNKYGFDAAFGGARRDEEKSRAKERIYSFRDRFHRWDP
                     KNQRPELWHNYNGQINKGESIRVFPLSNWTEQDIWQYIWLENIDIVPLYLAAERPVLE
                     RDGMLMMIDDNRIDLQPGEVIKKRMVRFRTLGCWPLTGAVESNAQTLPEIIEEMLVST
                     TSERQGRVIDRDQAGSMELKKRQGYF"
     gene            10722..11759
                     /gene="iap"
                     /locus_tag="NBI74_000708"
     CDS             10722..11759
                     /gene="iap"
                     /locus_tag="NBI74_000708"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase isozyme conversion
                     aminopeptidase"
                     /protein_id="HCD1931084.1"
                     /translation="MFSALRHRTAALALGVCFILPVHASSPKPGDFANTQARHIATFF
                     PGRMTGTPAEMLSADYIRQQFQQMGYRSDIRTFNSRYIYTARDNRKSWHNVTGSTVIA
                     AHEGKTPQQIIIMAHLDTYAPLSDADADANLGGLTLQGMDDNAAGLGVMLELAERLKN
                     TPTEYGIRFVATSGEEEGKLGAENLLKRMSDTEKKNTLLVINLDNLIVGDKLYFNSGV
                     KTPEAVRKLTRDRALAIARSHGIAATTNPGLNKNYPKGTGCCNDAEIFDKAGIAVLSV
                     EATNWNLGNKDGYQQRAKTAAFPAGNSWHDVRLDNQQHIDKALPGRIERRCRDVMRIM
                     LPLVKELAKAS"
     gene            complement(11885..12037)
                     /locus_tag="NBI74_000709"
     CDS             complement(11885..12037)
                     /locus_tag="NBI74_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Hok family toxin"
                     /protein_id="HCD1931085.1"
                     /translation="MLTKYALVAIIVLCCTVLGFTLMVGDSLCELSIRERGMEFKAVL
                     AYESKK"
     gene            complement(12303..13037)
                     /gene="cysH"
                     /locus_tag="NBI74_000710"
     CDS             complement(12303..13037)
                     /gene="cysH"
                     /locus_tag="NBI74_000710"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HCD1931086.1"
                     /translation="MSKLDLNALNELPKVDRILALAETNAQLEKLDAEGRVAWALDNL
                     PGEYVLSSSFGIQAAVSLHLVNQIRPDIPVILTDTGYLFPETYRFIDELTDKLKLNLK
                     VYRATESAAWQEARYGKLWEQGVEGIEKYNDINKVEPMNRALKELNAQTWFAGLRREQ
                     SGSRANLPVLAIQRGVFKVLPIIDWDNRTIYQYLQKHGLKYHPLWDEGYLSVGDTHTT
                     RKWEPGMAEEETRFFGLKRECGLHEG"
     gene            complement(13111..14823)
                     /gene="cysI"
                     /locus_tag="NBI74_000711"
     CDS             complement(13111..14823)
                     /gene="cysI"
                     /locus_tag="NBI74_000711"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001290736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="assimilatory sulfite reductase (NADPH)
                     hemoprotein subunit"
                     /protein_id="HCD1931087.1"
                     /translation="MSEKHPGPLVVEGKLTDAERMKLESNYLRGTIAEDLNDGLTGGF
                     KGDNFLLIRFHGMYQQDDRDIRAERAEQKLEPRHAMLLRCRLPGGVITTKQWQAIDKF
                     AGENTIYGSIRLTNRQTFQFHGILKKNVKPVHQMLHSVGLDALATANDMNRNVLCTSN
                     PYESQLHAEAYEWAKKISEHLLPRTRAYAEIWLDQEKVATTDEEPILGQTYLPRKFKT
                     TVVIPPQNDIDLHANDMNFVAIAENGKLVGFNLLVGGGLSIEHGNKKTYARTASEFGY
                     LPLEHTLAVAEAVVTTQRDWGNRTDRKNAKTKYTLERVGVETFKAEVERRAGIKFEPI
                     RPYEFTGRGDRIGWVKGIDDNWHLTLFIENGRILDYPGRPLKTGLLEIAKIHKGDFRI
                     TANQNLIIAGVPESEKAKIEKIAKESGLMNAVTPQRENSMACVSFPTCPLAMAEAERF
                     LPSFIDNIDNLMAKHGVSDEHIVMRVTGCPNGCGRAMLAEVGLVGKAPGRYNLHLGGN
                     RIGTRIPRMYKENITEPEILASLDELIGRWAKEREAGEGFGDFTVRAGIIRPVLDPAR
                     DLWD"
     gene            complement(14823..16622)
                     /gene="cysJ"
                     /locus_tag="NBI74_000712"
     CDS             complement(14823..16622)
                     /gene="cysJ"
                     /locus_tag="NBI74_000712"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent assimilatory sulfite reductase
                     flavoprotein subunit"
                     /protein_id="HCD1931088.1"
                     /translation="MTTQVPPSALLPLNPEQLARLQAATTDLTPTQLAWVSGYFWGVL
                     NQQPAALAATPAPAAEMPGITIISASQTGNARRVAEALRDDLLAAKLNVKLVNAGDYK
                     FKQIASEKLLIVVTSTQGEGEPPEEAVALHKFLFSKKAPKLENTAFAVFSLGDSSYEF
                     FCQSGKDFDSKLAELGGERLLDRVDADVEYQAAASEWRARVVDALKSRAPVAAPSQSV
                     ATGTVNEIHTSPYSKDAPLVASLSVNQKITGRNSEKDVRHIEIDLGDSDLRYQPGDAL
                     GVWYQNDPALVKELVELLWLKGDEPVTVEGKTLPLNEALQWHFELTVNTANIVENYAT
                     LTRSETLLPLVGDKAKLQHYAATTPIVDMVRFSPAQLDAEALINLLRPLTPRLYSIAS
                     SQAEVENEVHVTVGVVRYDVEGRARAGGASSFLADRVEEEGEVRVFIEHNDNFRLPTN
                     PETPVIMIGPGTGIAPFRAFMQQRAADEAPGKNWLFFGNPHFTEDFLYQVEWQRYVKE
                     GVLTRIDLAWSRDQKEKIYVQDKLREQGAELWRWINDGAHIYVCGDANRMAKDVEQAL
                     LEVIAEFGGMDTEAADEFLSELRVERRYQRDVY"
     gene            16941..17303
                     /gene="queD"
                     /locus_tag="NBI74_000713"
     CDS             16941..17303
                     /gene="queD"
                     /locus_tag="NBI74_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708563.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HCD1931089.1"
                     /translation="MSTTLFKDFTFEAAHRLPHVPQGHKCGRLHGHSFMVRLEITGEV
                     DPHTGWIIDFAELKAAFKPTYERLDHHYLNDIPGLENPTSEVLAKWIWDQVKPVVPLL
                     SAVMVKETCTAGCIYRGE"
     gene            17381..18652
                     /locus_tag="NBI74_000714"
     CDS             17381..18652
                     /locus_tag="NBI74_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HCD1931090.1"
                     /translation="MEDDCDIIIIGAGIAGTACALRCARAGLSVLLLERAEIPGSKNL
                     SGGRLYTHALAELLPQFHLTAPLERRITHESLSLLTPDGATTFSSLQPGGESWSVLRA
                     RFDPWLVAEAEKEGVECIPGATVDALYEENGRVCGVICGDDILHARYVVLAEGANSVL
                     AERHGLVTRPAGEAMALGIKEVLTLEPSAIEERFHLENNEGAALLFSGGICDDLPGGA
                     FLYTNQQTLSLGIVCPLSSLTQSSVPASELLTRFKTHPAVRPLIKNTELLEYGAHLVP
                     EGGLRSMPVQYAGNGWLLVGDALRSCVNTGISVRGMDMALTGAQAAAQTLISACQHRE
                     PQNLFPLYHHNVERSLLWDVLQRYQHVPALLQRPGWYRVWPGLMQDISRDLWDQGNTP
                     VPPLRQLLWRHLRRHGLWHLTGDVIRSLRCL"
     gene            18643..18903
                     /locus_tag="NBI74_000715"
     CDS             18643..18903
                     /locus_tag="NBI74_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HCD1931091.1"
                     /translation="MSVARNLWRAADAPHIVPTDSVERQTAQRLINACPAGLFSLTPE
                     GGLRVDYHGCLECGTCRLLCDESTLQQWRYPPSGFGITYRFG"
     gene            18920..19495
                     /locus_tag="NBI74_000716"
     CDS             18920..19495
                     /locus_tag="NBI74_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate responsive antiterminator"
                     /protein_id="HCD1931092.1"
                     /translation="MPLLHLLRQNPVIAAVKDNASLQLAIDSECQFISVLYGNICTIS
                     NIVKKIKNAGIYAFIHVDLLEGASNKEVVIQFLKLVTEADGIISTKASMLKAARAEGF
                     FCIHRLFIVDSISFHNIDKQVAQSNPDCIEILPGCMPKVLGWVTEKIRQPLIAGGLVC
                     DEEDARNAINAGVVALSTTNTGVWTLAKKLL"
     gene            complement(19643..20503)
                     /locus_tag="NBI74_000717"
     CDS             complement(19643..20503)
                     /locus_tag="NBI74_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417249.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HCD1931093.1"
                     /translation="MNIVIVTINQDHAAIASWLAAQDFSGCTLAHWQIEPQPMVAEQV
                     LDALVEQWQRTPADVVLFPPGAFGDELSTRLAWRLHGASICQVTSLDIPTVSVRKSHW
                     GNALTATLQTDKRPLCLSLARQAGADKNATLPSGMQQLNIVPGVLPDWLVSIENLKNV
                     IRDPLAEARRVLVVGQGGEADNQEIAMLAEKMGAEVGYSRARVMNGGVDAEKVIGISG
                     HLLAPEVCIVVGASGAAALMAGVRNSKFVVAINHDASAAVFSQADVGVVDDWKVVLEA
                     LVTNIHADCQ"
     gene            complement(20500..21279)
                     /locus_tag="NBI74_000718"
     CDS             complement(20500..21279)
                     /locus_tag="NBI74_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HCD1931094.1"
                     /translation="MNILLAFKAEPDAGMLAEKEWQAAAQGNSGPDVSLLRSLLGADE
                     QAAAALLLAQRKNGTPMSLTALSMGDERALHWLRYLMALGFEEAVLLETAADLRFAPE
                     FVARHIAEWQRQNPLDLIITGCQSSEGQNGQTPFLLAEMLVWPCFTQVERFTLDAPFI
                     TLEQRTEHGVRCCRVRLPAVIAVRQCGEVALPVPGMRQRMAAGKAAIIRKTVAVELPA
                     MQCLQLARAEQRRGATLIGGQTVAEKAQKLWQDYLRQRMQP"
     gene            complement(21257..22666)
                     /locus_tag="NBI74_000719"
     CDS             complement(21257..22666)
                     /locus_tag="NBI74_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCD1931095.1"
                     /translation="MTVRCLFGFSGEKPFLLPDNEGVKMNTSPVRMDDLPLNRFHCRI
                     AALTFGAHLTDGYVLGVIGYAIIQLTPAMQLTPFMAGMIGGSALLGLFLGSLVLGWIS
                     DHIGRQKIFTFSFMLITLASFLQFFATTPEHLIGLRILIGIGLGGDYSVGHTLLAEFS
                     PRRHRGILLGAFSVVWTVGYVLASIAGHHFISESPEAWRWLLASAALPALLITLLRWG
                     TPESPRWLLRQGRFAEAHAIVHRYFGPHVLLGDEVATATHKHIKTLFSSRYWRRTAFN
                     SVFFVCLVIPWFVIYTWLPTIAQTIGLEDALTASLMLNALLIVGALLGLVLTHLLAHR
                     KFLLGSFLLLAATLVVMACLPSGSSLTLLLFVLFSTTISAVSNLVGILPAESFPTDIR
                     SLGVGFATAMSRLGAAVSTGLLPWVLAQWGMQATLLLLAAVLLVGFVVTWLWAPETKA
                     LPLVAAGNVGGANEHSVSV"
     gene            complement(22688..24142)
                     /locus_tag="NBI74_000720"
     CDS             complement(22688..24142)
                     /locus_tag="NBI74_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HCD1931096.1"
                     /translation="MSLSRVAIVDQLKEIVGADRVITDETVLKKNSIDRFRKFPDIHG
                     IYTLPIPAAVVKLGSTEQVSRVLNFMNAHKINGVPRTGASATEGGLETVVENSVVLDG
                     SAMNQIINIDIENMQATAQCGVPLEVLENALREKGYTTGHSPQSKPLAQMGGLVATRS
                     IGQFSTLYGAIEDMVVGLEAVLADGTVTRIKNVPRRAAGPDIRHIIIGNEGALCYITE
                     VTVKIFKFTPENNLFYGYILEDMKTGFNILREIMVEGYRPSIARLYDAEDGTQHFTHF
                     ADGKCVLIFMAEGNPRIAKATGEGIAEIVARYPQCQRVDSKLIETWFNNLNWGPDKVA
                     AERVQILKTGNMGFTTEVSGCWSCIHEIYESVINRIRTEFPHADDITMLGGHSSHSYQ
                     NGTNMYFVYDYNVVDCKPEEEIDKYHNPLNKIICEETIRLGGSMVHHHGIGKHRVHWS
                     KLEHGSAWALLEGLKKQFDPNGIMNTGTIYPIEK"
     gene            complement(24212..24997)
                     /locus_tag="NBI74_000721"
     CDS             complement(24212..24997)
                     /locus_tag="NBI74_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCD1931097.1"
                     /translation="MSIESLNAFSMDFFSLKGKTAIVTGGNSGLGQAFAMALAKAGAN
                     VFIPSFVKDNGETKEMIEKQGVEVDFMQVDITAEGAPQKIIAACCERFGTVDILVNNA
                     GICKLNKVLDFGRADWDPMIDVNLTAAFELSYEAAKIMIPQKSGKIINICSLFSYLGG
                     QWSPAYSATKHALAGFTKAYCDELGQYNIQVNGIAPGYYATDITLATRSNPETNQRVL
                     DHIPANRWGDTQDLMGAAVFLASPASNYVNGHLLVVDGGYLVR"
     gene            25316..26593
                     /locus_tag="NBI74_000722"
     CDS             25316..26593
                     /locus_tag="NBI74_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCD1931098.1"
                     /translation="MQHNSYRRWITLAIISFSGGVSFDLAYLRYIYQIPMAKFMGFSN
                     TEIGLIMSTFGIAAIIFYAPSGVIADKFSHRKMITSAMIITGLLGLLMATYPPLWVML
                     CIQVAFAITTILMLWSVSIKAASLLGDHSEQGKIMGWMEGLRGVGVMSLAVFTMWVFS
                     RFAPDDSASLKTVIIIYSVVYILLGILCWFFVSDNNNLRSANNEEKQSFQLSDILAVL
                     RISTTWYCSMVIFGVFTIYAILSYSTNYLTEMYGMSLVAASYMGIVINKIFRALCGPL
                     GGIITTYSKVKSPTRVVQILSIIGLLALTALLVTNSNPQSVAMGIGLILLLGFTCYAS
                     RGLYWACPGEARTPSYIMGTTVGICSVIGFLPDVFVYPIIGYWQDTLPAAEAYRNMWL
                     MGMAALGMVIIFTFLLFQKIRTADSAPAMASSK"
     gene            26620..28098
                     /locus_tag="NBI74_000723"
     CDS             26620..28098
                     /locus_tag="NBI74_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HCD1931099.1"
                     /translation="MSKKYIIGIDGGSQSTKVVMYDLEGNVVCEGKGLLQPMHTPDAD
                     TAEHPDDDLWASLCFASHDLMSQFAGNKEDIVGIGLGSIRCCRALLKADGTPAAPLIS
                     WQDARVTRPYEHTNPDVAYVTSFSGYLTHRLTGEFKDNIANYFGQWPVDYKSWAWSED
                     ATVMEKFNIPRQMLFDVQMPGTILGHITPQAALATHFPAGLPVVCTTSDKPVEALGAG
                     LLDDETAVISLGTYIALMMNGKALPKDPVAYWPIMSSIPQTLLYEGYGIRKGMWTVSW
                     LRDMLGESLIQDAKAQDLSPEDLLNKKASCVPPGCNGLMTVLDWLTNPWEPYKRGIMI
                     GFDSSMDYAWIYRSILESVALTLKNNYDNMCHEMNHFAKHVIITGGGSNSDLFMQIFA
                     DVFNLPARRNAINGCASLGAAINTAVGLGLYPDYTTAVDKMVRVKDIFIPIESNAKRY
                     DAMNKGIFKDLTKHTDVILKKSYEVMHGELGNADSIQSWSNA"
     gene            28263..29183
                     /locus_tag="NBI74_000724"
     CDS             28263..29183
                     /locus_tag="NBI74_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001461526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoimidazole riboside kinase"
                     /protein_id="HCD1931100.1"
                     /translation="MSTRVWVLGDAVVDLLPESQGRLLQCPGGAPANVAVGIARLGGK
                     SAFIGKVGDDPFGRFMYQTLSTENVDTRYMSLDPQHRTSIVAVGLDEQGERNFTFMVR
                     PSADLFLQPDDLPAFGPGEWLHLCSIALSAEPSRSTAFLAMEKIRQAGGNISFDPNIR
                     SDLWQSEALLRKYLDRALSLANIAKLSEEELLFISDESQVQQGAYSLVQRYPLTLLLI
                     TQGKNGVLVYFQGQFIHYPAKPVSVVDTTGAGDAFVAGLLAGLADSGIPTNTRQLERI
                     IAQAQICGALATTAKGAMTALPRQHDLPSQ"
     gene            29351..30868
                     /locus_tag="NBI74_000725"
     CDS             29351..30868
                     /locus_tag="NBI74_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003857084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate porin"
                     /protein_id="HCD1931101.1"
                     /translation="MYKKTTLAVLIALLTGATTVHAQTDISSIESRLAALEQRLKNAE
                     SRAQAAEARAKTAELQVQKLAETQQQNQLTTQEVAQRTVQLEQKSAENSGFEFHGYAR
                     SGLLMNDAASSSKSGPYLTPAGETGGAVGRLGNEADTYVELNVEHKQTLDNGATTRFK
                     AMLADGQRDYNDWTGGSSNLNIRQAFAELGALPSFTGAFKDSTVWAGKRFDRDNFDIH
                     WLDSDVVFLAGTGGGIYDVKWNETFRSNFSLYGRNFGDLDDIDNNVQNYILTMNHYAG
                     PFQLMVSGLRAKDNDDRKDANGDLIQTDAANTGVHALVGLHNDTFYGLREGTAKTALL
                     YGHGLGAEVKGIGSDGALLSEANTWRFASYGTTPLGSGWYVAPAILAQSSKDRYVKGD
                     SYEWVTFNTRLIKEVTQNFALAFEGSYQYMDLKPKGYQNHNAVNGSFYKLTFAPTLKA
                     NDINNFFSRPELRLFATWMDWSSKLDDFASNDAFGSSGFNTGGEWNFGVQMETWF"
     gene            30929..32299
                     /locus_tag="NBI74_000726"
     CDS             30929..32299
                     /locus_tag="NBI74_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sucrose transporter subunit IIBC"
                     /protein_id="HCD1931102.1"
                     /translation="MDFDKIAQSLLPLLGGKENIASAAHCATRLRLVLVDDTLADQHA
                     IGQIDGVKGCFRNSGQMQIIFGTGVVNKVYAAFIQVAGISESSKADTARLAAQKLNPF
                     QRIARLLSNIFVPIIPAIVASGLLMGLLGMVKTYGWVNADNAIYILLDMCSSAAFIIL
                     PILIGFTAAREFGGNPYLGATLGGILTHPALTNAWGVAAGFQTMNFFGFEIAMIGYQG
                     TVFPVLLAVWFMSIVEKQLRRFIPDALDLILTPFLTVVISGFIALLIIGPAGRALGDG
                     ISFVLSTLIAHAGWLAGLLFGGLYSAIVITGIHHSFHAIEAGLLGNPAIGVNFLLPIW
                     AMANVAQGGACLAVWFKTKDTKIKAITLPSAFSAMLGITEAAIFGINLRFVKPFIAAL
                     IGGAAGGAWVVSVHVYMTAVGLTAIPGMAIVQPTSLVNYIIGMVIAFAVAFSLSLLLK
                     YKTDEE"
     gene            32299..33708
                     /locus_tag="NBI74_000727"
     CDS             32299..33708
                     /locus_tag="NBI74_000727"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001466359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HCD1931103.1"
                     /translation="MSMPSQMPAILQAVMKGQSKALADAHYPCWHLAPVTGLMNDPNG
                     FCWSGGRYHLFYQWNPLACDHKYKCWGHWSSTDLLHWQHEPLALMPDKEYDRNGCYSG
                     SAVNNQGVLTLCYTGNVKFDDGSRTAWQCLATENNQGGFDKLGPVIPLPDGYTGHVRD
                     PKVWKHNSQWYMVLGAQDKEKRGKVLLYSSVDLYTWSFHGEIAGNGLNEIDNAGYMWE
                     CPDLFALDGEYILLCCPQGMAREHERYLNTYPCAWLHGQFDYETGKFTHGAFSELDAG
                     FEFYAPQTMEAPDGRRLLVGWMGVPDGEEMLQPTRKHHWQHQMTCFRELSFQKGKLFQ
                     MPIRELAQLREAEHFWQGKADHAPHVEIERLEMDIIPSGELYLNFGNALALHLNDDGI
                     QLQRRSLAGQEKLTRYWRGSVTSLKILCDSSSVEIFINNGEGVMSNRYFPHHPASLIL
                     QGESDVTLRYWSLRACMVE"
     gene            33734..34741
                     /locus_tag="NBI74_000728"
     CDS             33734..34741
                     /locus_tag="NBI74_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCD1931104.1"
                     /translation="MRKTKRVTIKDIAELAGVSKATASLVLNGRGKELRVAQETRERV
                     LSIAQQQHYQPSIHARSLRDNRSHTIGLVVPEITNYGFAVFSHELETLCREAGVQLLI
                     SCTDENPSQETMVVNNMIARQVDGLIVASSMLHDNDYQKLSEQLPVVLFDRHMNGSTL
                     PLVITDSVSPTAALVADIARSHPDEFYFLGGQPRLSPTRDRLEGFTQGLQQAGVTLQP
                     EWIIHGNYHPSSGYEMFAALCARLGRPPKALFTAACGLLEGVLRYMSQYNLLDSKIHL
                     ASFDDHYLYDSLSVRIDTIQQDNRQLAFHCFELISQLIEGETPSPLQRYLPASLQKRY
                     R"
     gene            complement(34826..35497)
                     /gene="queE"
                     /locus_tag="NBI74_000729"
     CDS             complement(34826..35497)
                     /gene="queE"
                     /locus_tag="NBI74_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HCD1931105.1"
                     /translation="MQYPINEMFQTLQGEGYFTGVPAIFIRLQGCPVGCAWCDTKHTW
                     EKLEDQEVSLFSILAKTKESDKWGAASSEDLLAVIGRQGYTARHVVITGGEPCIHDLL
                     PLTDLLEKNGFSCQIETSGTHEVRCTPNTWVTVSPKLNMRGGYEVLSQALERANEIKH
                     PVGRVRDIEALDELLATLTDDKPRVIALQPISQKEDATRLCIDTCIARNWRLSMQTHK
                     YLNIA"
     gene            35636..35777
                     /locus_tag="NBI74_000730"
                     /pseudo
     CDS             35636..35777
                     /locus_tag="NBI74_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791252.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            35791..36663
                     /locus_tag="NBI74_000731"
     CDS             35791..36663
                     /locus_tag="NBI74_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgcG family protein"
                     /protein_id="HCD1931106.1"
                     /translation="MRYFILIFTLVCSFVAAQPTIVPQLQQQVTDLTSSLNSQEKKEL
                     TRKLESIFNNTQVQIAVLIIPTTKDETIEQYAARVFDNWRLGDAKRNDGILIIVAWSD
                     RTVRIQVGYGLEEKVTDALAGDIIRSNMIPAFKQQQLAKGLELAIIALNNRLTSQHQY
                     SVNPSESESASSSDHYYFAIFWVFAVMFFPFWFFHQGSNFCRACKSSVCISAIYLLDL
                     FLFSDKTFSIAVFFFFFTFTTIMVFTCLCVLQKKASGRSYHSDNSGSGGGSSGGGFSG
                     GGGSSGGGGASGRW"
     gene            complement(36723..38021)
                     /gene="eno"
                     /locus_tag="NBI74_000732"
     CDS             complement(36723..38021)
                     /gene="eno"
                     /locus_tag="NBI74_000732"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HCD1931107.1"
                     /translation="MSKIVKIIGREIIDSRGNPTVEAEVHLEGGFVGMAAAPSGASTG
                     SREALELRDGDKSRFLGKGVTKAVAAVNGPIAQALIGKDAKDQAGIDKIMIDLDGTEN
                     KSKFGANAILAVSLANAKAAAAAKGMPLYEHIAELNGTPGKYSMPVPMMNIINGGEHA
                     DNNVDIQEFMIQPVGAKTVKEAIRMGSEVFHHLAKVLKAKGMNTAVGDEGGYAPNLGS
                     NAEALAVIAEAVKAAGYELGKDITLAMDCAASEFYKDGKYVLAGEGNKAFTSEEFTHF
                     LEELTKQYPIVSIEDGLDESDWDGFAYQTKVLGDKIQLVGDDLFVTNTKILKEGIEKG
                     IANSILIKFNQIGSLTETLAAIKMAKDAGYTAVISHRSGETEDATIADLAVGTAAGQI
                     KTGSMSRSDRVAKYNQLIRIEEALGEKAPYNGRKEIKGQA"
     gene            complement(38109..39746)
                     /gene="pyrG"
                     /locus_tag="NBI74_000733"
     CDS             complement(38109..39746)
                     /gene="pyrG"
                     /locus_tag="NBI74_000733"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase (glutamine hydrolyzing)"
                     /protein_id="HCD1931108.1"
                     /translation="MTTNYIFVTGGVVSSLGKGIAAASLAAILEARGLNVTIMKLDPY
                     INVDPGTMSPIQHGEVFVTEDGAETDLDLGHYERFIRTKMSRRNNFTTGRIYSDVLRK
                     ERRGDYLGATVQVIPHITNAIKERVLEGGEGHDVVLVEIGGTVGDIESLPFLEAIRQM
                     AVEIGREHTLFMHLTLVPYMAASGEVKTKPTQHSVKELLSIGIQPDILICRSDRAVPA
                     NERAKIALFCNVPEKAVISLKDVDSIYKIPGLLKSQGLDDYICKRFSLNCPEANLSEW
                     EQVIFEEANPVSEVTIGMVGKYIELPDAYKSVIEALKHGGLKNRVSVNIKLIDSQDVE
                     TRGVEILKGLDAILVPGGFGYRGVEGMITTARFARENNIPYLGICLGMQVALIDYARH
                     VANMENANSTEFVPDCKYPVVALITEWRDENGNVEVRSEKSDLGGTMRLGAQQCQLVD
                     DSLVRQLYNAPTIVERHRHRYEVNNMLLKQIEDAGLRVAGRSGDDQLVEIIEVPNHPW
                     FVACQFHPEFTSTPRDGHPLFAGFVKAASEFQKRQAK"
     gene            complement(39974..40765)
                     /gene="mazG"
                     /locus_tag="NBI74_000734"
     CDS             complement(39974..40765)
                     /gene="mazG"
                     /locus_tag="NBI74_000734"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HCD1931109.1"
                     /translation="MNQIDRLLTIMQRLRDPENGCPWDKEQTFATIAPYTLEETYEVL
                     DAIAREDFDDLRGELGDLLFQVVFYAQIAQEEGRFDFNDICAAISDKLERRHPHVFAD
                     SSAENSSEVLARWEQIKTEERAQKAQHSALDDIPRSLPALMRAQKIQKRCANVGFDWT
                     TLGPVVDKVYEEIDEVMYEARQAVVDQAKLEEEMGDLLFATVNLARHLGTKAEIALQK
                     ANEKFERRFREVERIVAARGLEMTGVDLETMEEVWQQVKRQEIDL"
     gene            complement(40861..43095)
                     /locus_tag="NBI74_000735"
     CDS             complement(40861..43095)
                     /locus_tag="NBI74_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase"
                     /protein_id="HCD1931110.1"
                     /translation="MVAVRSAHINKAGEFDPEKWIASLGITSQKSCECLAETWAYCLQ
                     QTQGHPDASLLLWRGVEMVEILSTLSMDIDTLRAALLFPLADANVVSEDVLRESVGKS
                     VVNLIHGVRDMAAIRQLKATHTDSVSSEQVDNVRRMLLAMVDDFRCVVIKLAERIAHL
                     REVKDAPEDERVLAAKECTNIYAPLANRLGIGQLKWELEDYCFRYLHPTEYKRIAKLL
                     HERRLDREHYIEEFVGHLRAEMKAEGVKAEVYGRPKHIYSIWRKMQKKNLAFDELFDV
                     RAVRIVAERLQDCYAALGIVHTHYRHLPDEFDDYVANPKPNGYQSIHTVVLGPGGKTV
                     EIQIRTKQMHEDAELGVAAHWKYKEGAAAGGARSGHEDRIAWLRKLIAWQEEMADSGE
                     MLDEVRSQVFDDRVYVFTPKGDVVDLPAGSTPLDFAYHIHSDVGHRCIGAKIGGRIVP
                     FTYQLQMGDQIEIITQKQPNPSRDWLNPNLGYVTTSRGRSKIHAWFRKQDRDKNILAG
                     RQILDDELEHLGISLKEAEKHLLPRYNFNDVDELLAAIGGGDIRLNQMVNFLQSQFNK
                     PSAEEQDAAALKQLQQKSYTPQNRSKDNGRVVVEGVGNLMHHIARCCQPIPGDEIVGF
                     ITQGRGISVHRADCEQLAELRSHAPERIVDAVWGESYSAGYSLVVRVVANDRSGLLRD
                     ITTILANEKVNVLGVASRSDTKQQLATIDMTIEIYNLQVLGRVLGKLNQVPDVIDARR
                     LHGS"
     gene            complement(43143..44444)
                     /gene="rlmD"
                     /locus_tag="NBI74_000736"
     CDS             complement(43143..44444)
                     /gene="rlmD"
                     /locus_tag="NBI74_000736"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HCD1931111.1"
                     /translation="MAQFYSAKRRTTTRQIITVSVNDLDSFGQGVARHNGKTLFIPGL
                     LPQENAEVTVTEDKKQYARAKVVRRLSDSPERETPRCPHFGVCGGCQQQHASVDLQQR
                     SKSAALARLMKHEVSEVIADVPWGYRRRARLSLNYLPKTQQLQMGFRKAGSSDIVDVK
                     QCPILVPQLEALLPKVRACLGSLQAMRHLGHVELVQATSGTLMILRHTAPLSSADREK
                     LECFSHSEGLDLYLAPDSEILETVSGEMPWYDSNGLRLTFSPRDFIQVNAGVNQKMVA
                     RALEWLDVEPEDRVLDLFCGMGNFTLPLATQAASVVGVEGVPALVEKGQQNARLNGLQ
                     NVTFYHENLEEDVTKQPWAKNGFDKVLLDPARAGAAGVMQQIIKLEPIRIVYVSCNPA
                     TLARDSEALLKAGYTIARLAMLDMFPHTGHLESMVLFSRVK"
     gene            44501..47257
                     /gene="barA"
                     /locus_tag="NBI74_000737"
     CDS             44501..47257
                     /gene="barA"
                     /locus_tag="NBI74_000737"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase BarA"
                     /protein_id="HCD1931112.1"
                     /translation="MTNYSLRARMMILILAPTVLIGLLLSIFFVVHRYNDLQRQLEDA
                     GASIIEPLAVSTEYGMSLQNRESIGQLISVLHRRHSDIVRAISVYDENNRLFVTSNFH
                     LDPSSMQLGSNVPFPRQLTVTRDGDIMILRTPIISESYSPDESPSSDAKNSQNMLGYI
                     ALELDLKSVRLQQYKEIFISSVMMLFCIGIALIFGWRLMRDVTGPIRNMVNTVDRIRR
                     GQLDSRVEGFMLGELDMLKNGINSMAMSLAAYHEEMQHNIDQATSDLRETLEQMEIQN
                     VELDLAKKRAQEAARIKSEFLANMSHELRTPLNGVIGFTRLTLKTELTPTQRDHLNTI
                     ERSANNLLAIINDVLDFSKLEAGKLILESIPFPLRSTLDEVVTLLAHSSHDKGLELTL
                     NIKSDVPDNVIGDPLRLQQIITNLVGNAIKFTENGNIDILVEKRALSNTKVQIEVQIR
                     DTGIGIPERDQSRLFQAFRQADASISRRHGGTGLGLVITQKLVNEMGGDISFHSLPNR
                     GSTFWFHINLDLNPNIIIEGPSTQCLAGKRLAYVEPNSAAAQCTLDILSETPLEVVYS
                     PTFSALPPAHYDMMLLGIAVTFREPLTMQHERLAKAVSMTDFLMLALPCHAQVNAEKL
                     KQDGIGACLLKPLTPTRLLPALTEFYHHKQNTLLPVTDESKLAMTVMAVDDNPANLKL
                     IGALLEDMVQHVELCDSGHQAVERAKQMPFDLILMDIQMPDMDGIRACELIHQLPHQQ
                     QTPVIAVTAHAMAGQKEKLLGAGMSDYLAKPIEEERLHNLLLRYKPGSGISSRVVTPE
                     VNEIVVNPNATLDWQLALRQAAGKTDLARDMLQMLLDFLPEVRNKVEEQLVGENPEGL
                     VDLIHKLHGSCGYSGVPRMKNLCQLIEQQLRSGTKEEDLEPELLELLDEMDNVAREAS
                     KILG"
     gene            complement(47488..48828)
                     /gene="gudD"
                     /locus_tag="NBI74_000738"
     CDS             complement(47488..48828)
                     /gene="gudD"
                     /locus_tag="NBI74_000738"
                     /EC_number="4.2.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucarate dehydratase"
                     /protein_id="HCD1931113.1"
                     /translation="MSSQFTTPVVTEMQVIPVAGHDSMLMNLSGAHAPFFTRNIVIIK
                     DNSGHTGVGEIPGGEKIRKTLEDAIPLVVGKTLGEYKNVLTLVRNTFADRDAGGRGLQ
                     TFDLRTTIHVVTGIEAAMLDLLGQHLGVNVASLLGDGQQRSEVEMLGYLFFVGNRKAT
                     PLPYQSQPDDQCDWYRLRHEEAMTPDAVVRLAEAAYEKYGFNDFKLKGGVLAGEEEAE
                     SIVALAQRFPQARITLDPNGAWSLNEAIKIGTYLKGSLAYAEDPCGAEQGFSGREVMA
                     EFRRATGLPTATNMIATDWRQMGHTLSLQSVDIPLADPHFWTMQGSVRVAQMCHEFGL
                     TWGSHSNNHFDISLAMFTHVAAAAPGKITAIDTHWIWQEGNQRLTKEPLEIKGGLVQV
                     PEKPGLGVEIDMDQVMKAHELYQKHGLGARDDAMGMQYLIPGWTFDNKRPCMVR"
     gene            complement(48849..50189)
                     /locus_tag="NBI74_000739"
     CDS             complement(48849..50189)
                     /locus_tag="NBI74_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucarate dehydratase"
                     /protein_id="HCD1931114.1"
                     /translation="MTTQSSPVITDMKVIPVAGHDSMLLNIGGAHNAYFTRNIVVLTD
                     NAGHTGIGEAPGGEVIYQTLVGAIPMVLGQEVARLNKVVQQVHKGNQAADFDTFGKGA
                     WTFELRVNAVAALEAALLDLLGQALNVPVCELLGPGKQRDAITVLGYLFYIGDRTKTD
                     LPYLENTPGNHEWYQLRHQKAMNSEAVVRLAEASQDRYGFKDFKLKGGVLPGEQEIDT
                     VRALKKRFPDARITVDPNGAWLLDEAISLCKGLNDVLTYAEDPCGAEQGFSGREVMAE
                     FRRATGLPVATNMIATNWREMGHAVMLNAVDIPLADPHFWTLSGAVRVAQLCDDWGLT
                     WGCHSNNHFDISLAMFTHVGAAAPGNPTAIDTHWIWQEGDCRLTKNPLEIKNGKIAVP
                     DAPGLGVELDWEQVQKAHEAYKRLPGGARNDAGPMQYLIPGWTFDRKRPVFGRH"
     gene            complement(50191..51543)
                     /gene="gudP"
                     /locus_tag="NBI74_000740"
     CDS             complement(50191..51543)
                     /gene="gudP"
                     /locus_tag="NBI74_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GudP"
                     /protein_id="HCD1931115.1"
                     /translation="MSSLSQAASSVEKRTNARYWIVVMLFIVTSFNYGDRATLSIAGS
                     EMAKDIGLDPVGMGYVFSAFSWAYVIGQIPGGWLLDRFGSKRVYFWSIFIWSMFTLLQ
                     GFVDIFSGFGIIVALFTLRFLVGLAEAPSFPGNSRIVAAWFPAQERGTAVSIFNSAQY
                     FATVIFAPIMGWLTHEVGWSHVFFFMGGLGIVISFIWLKVIHEPNQHPGVNQKELEYI
                     AAGGALINMDQQNTKVKVPFSVKWGQIKQLLGSRMMIGVYIGQYCINALTYFFITWFP
                     VYLVQARGMSILKAGFVASVPAVCGFIGGVLGGIISDWLMRRTGSLNIARKTPIVMGM
                     LLSMVMVFCNYVNVEWMIIGFMALAFFGKGIGALGWAVMADTAPKEISGLSGGLFNMF
                     GNISGIVTPIAIGYIVGTTGSFNGALIYVGVHALIAVLSYLVLVGDIKRIELKPVAGQ
                     "
     gene            complement(51977..52426)
                     /locus_tag="NBI74_000741"
     CDS             complement(51977..52426)
                     /locus_tag="NBI74_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HCD1931116.1"
                     /translation="MAEIGIFVGTMYGNSLLVAEEAEAILTAQGHKATVFEDPELSDW
                     LPYQDKYVLVVTSTTGQGDLPDSIVPLFQGIKDTLGFQPNLRYGVIALGDSSYVNFCN
                     GGKQFDALLQEQSAQRVGEMLLIDASENPEPETESNPWVEHWGTMLS"
     gene            complement(52444..53226)
                     /gene="truC"
                     /locus_tag="NBI74_000742"
     CDS             complement(52444..53226)
                     /gene="truC"
                     /locus_tag="NBI74_000742"
                     /EC_number="5.4.99.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(65) synthase TruC"
                     /protein_id="HCD1931117.1"
                     /translation="MLEILYQDEWLVAVNKPSGWLVHRSWLDRDEKVVVMQTVRDQIG
                     QHVFTAHRLDRPTSGVLLMGLSSEAGRLLAQQFEQHQIQKRYHAIVRGWLMEEAVLDY
                     PLVEELDKIADKFAREDKGPQPAVTHYRGLATVEMPVATGRYPTTRYGLVELEPKTGR
                     KHQLRRHLAHLRHPIIGDSKHGDLRQNRSGAEHFGLQRLMLHASQLSLTHPFTGEPLA
                     IHAGLDDTWMQALSQFGWRGLLPENERVEFSAPSGQDGEISS"
     gene            complement(53226..53555)
                     /locus_tag="NBI74_000743"
     CDS             complement(53226..53555)
                     /locus_tag="NBI74_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqcC family protein"
                     /protein_id="HCD1931118.1"
                     /translation="MTTHDRVRLQLQALEALLREHQHWRNDEPQPHQFNSTQPFFMDT
                     MEPLEWLQWVLIPRMHDLLDNNQPLPGAFAVAPYYEMALATDHPQRALILAELEKLDA
                     LFADDAS"
     gene            complement(54177..54722)
                     /gene="syd"
                     /locus_tag="NBI74_000744"
     CDS             complement(54177..54722)
                     /gene="syd"
                     /locus_tag="NBI74_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SecY-interacting protein"
                     /protein_id="HCD1931119.1"
                     /translation="MDDLTAQALKDFTARYCDAWHEEHKSWPLSEELYGVPSPCIIST
                     TEDAVYWQPQPFTGEQNVNAVERAFDLVIQPTIHTFYTTQFAGDMHAQFGDIKLTLLQ
                     TWSEDDFRRVQENLIGHLVTQKRLKLPPTLFIATLEEELEVISVCNLSGEVCKETLGT
                     RKRTHLASNLAEFLNQLKPLL"
     gene            54790..55638
                     /gene="queF"
                     /locus_tag="NBI74_000745"
     CDS             54790..55638
                     /gene="queF"
                     /locus_tag="NBI74_000745"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HCD1931120.1"
                     /translation="MSSYANHQALAGLTLGKSTDYRDTYDASLLQGVPRSLNRDPLGL
                     KADNLPFHGTDIWTLYELSWLNAKGLPQVAVGHVELDYTSANLIESKSFKLYLNSFNQ
                     TRFNNWDEVRQTLERDLSTCAQGKVSVALYRLDELEGQPIGHFNGTCIDDQDITIDNY
                     EFTTDYLENATSGEKVVEETLVSHLLKSNCLITHQPDWGSIQIQYRGRQIDREKLLRY
                     LVSFRHHNEFHEQCVERIFNDLLRFCQPEKLSVYARYTRRGGLDINPWRSNSDFVPST
                     TRLVRQ"
     gene            55750..57114
                     /gene="ppnN"
                     /locus_tag="NBI74_000746"
                     /gene_synonym="ygdH"
     CDS             55750..57114
                     /gene="ppnN"
                     /locus_tag="NBI74_000746"
                     /gene_synonym="ygdH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide 5'-monophosphate nucleosidase"
                     /protein_id="HCD1931121.1"
                     /translation="MITHISPLGSMDMLSQLEVDMLKRTASSDLYQLFRNCSLAVLNS
                     GSLTDNSKELLSRFENFDINVLRRERGVKLELINPPEEAFVDGRIIRALQANLFAVLR
                     DILFVYGQIHNTVRFPNLNLDNSVHITNLVFSILRNARALHVGEAPNMVVCWGGHSIN
                     ENEYLYARRVGNQLGLRELNICTGCGPGAMEAPMKGAAVGHAQQRYKDSRFIGMTEPS
                     IIAAEPPNPLVNELIIMPDIEKRLEAFVRIAHGIIIFPGGVGTAEELLYLLGILMNPA
                     NKDQVLPLILTGPKESADYFRVLDEFVVHTLGENARRHYRIIIDDAAEVARQMKKSMP
                     LVKENRRDTGDAYSFNWSMRIAPDLQMPFEPSHENMANLKLYPDQPVEVLAADLRRAF
                     SGIVAGNVKEVGIRAIEEFGPYKINGDKEIMRRMDDLLQGFVAQHRMKLPGSAYIPCY
                     EICT"
BASE COUNT        13932 a        15003 c        13999 g        14289 t
ORIGIN      
        1 tattattcag gatatggtca cactaaactg gttgctgaaa aagtggcaca aggcgcccat
       61 gcagatttaa tttcgattga taacgaagga aatatcactg agcaggagtg ggaaaaactg
      121 gacgcagccg atgccattat cttcggtgct cccacctata tgggcggtgc gccatggcaa
      181 ttcaagaaat ttgctgatgc cagttccaaa gcgtggttta cacgtaaatg gcaggataaa
      241 gtcttcgggg gtttcaccaa cagcgccagc ctgaatggcg ataaacaagt tacgctgatc
      301 tatctgcaaa cgctggcttc acagcatggc ggtctgtggg tgagtttagg tcaggctcct
      361 gccaatgtgc ttgcgtcaac cagggaagat gtaaacaatc tgggcggttc ggtcggtttg
      421 ctggtgcaat ctccagccga tgcgggtgca gagcaaatcc ctacgggcga tctggataca
      481 gccgtgaaat atggtgaacg cgtggcgaca attactgcgc gcttaaaatg atgattgccg
      541 aatgtgatgc tgacgcgtct atccggtctt ctagatctaa aaagtgccgt cccctggtca
      601 gtcagataaa gttttaacgc tgtatccgat aacagttcaa catgtaccaa aagaaaaaaa
      661 gccagcctcg cttgagactg gcctttctga cagatgctta cttattcgcg gaacagcgct
      721 tcgatattca gcccctgcgt ttgcaggatt tcgcgcaaac ggcgcaggcc ttcaacctga
      781 atctggcgaa cacgttcacg ggtgaggcca atttcacgac ctacatcttc cagtgttgcc
      841 gcttcgtacc ccagcaaacc gaatcgacgt gccagcactt cacgctgttt ggcgttcagc
      901 tcgaacagcc atttgacgat gctctgcttc atatcgtcat cttgcgtggt atcttccgga
      961 ccattctctt tttcatcggc caggatgtcc agcaatgctt tttcggaatc accacccagc
     1021 ggggtgtcta ccgaggtaat gcgctcgtta agacgaagca tacggctgac gtcatcaact
     1081 ggcttatcca gttgctctgc gatctcttcc gcacttggtt cgtggtccag cttatgggac
     1141 aactcacgtg cggttcgcag gtaaacgttc agctccttta cgatgtgaat cggcaaacga
     1201 atagtacggg tttggttcat aatcgcccgt tcgatcgtct ggcgaatcca ccaggttgcg
     1261 tatgttgaga agcggaaacc acgttccggg tcaaacttct ctaccgcgcg gatcagcccc
     1321 aggttgccct cttcgatcag gtccagcaac gccagaccac gattgccata acggcgggca
     1381 atttttacca ccagacgcaa gttactctcg atcatccggc ggcgagaggc gacatctcca
     1441 cgcagtgcgc gacgcgcaaa ataaacttct tcttcggccg ttaacagtgg tgaataacca
     1501 atctcaccaa ggtaaagctg agtcgcgtcc agcacacgct gtgtggctcc ctgcgataac
     1561 agttcctctt cggccaaatc gttatcactg ggttcctctt ctactaaggc cttttcgtca
     1621 aaaacctcaa ctccgttctc atcaaattcc gcatcttcat ttaaatcatg aactttcagc
     1681 gtattctgac tcataaggtg gctcctaccc gtgatccctt gacggaacat tcaagcaaaa
     1741 gcctggttcc gccgatttat cgctgcggca aataacgcag cgggtttacg gatttcccct
     1801 tgtaacgaat ttcaaaatgc aagcgtgttg aactggttcc ggtgctaccc atggttgcta
     1861 ttttttgccc cgccttcact tcttgttgtt cccggaccag cattgtgtcg ttatgggcgt
     1921 aggcactcag gtaatcatca ttatgtttga tgataatcag attaccgtag ccgcgcagcg
     1981 cgttaccggc ataaacaacg cggccatctg cggtcgcgat aattgcctgt cctttgctgc
     2041 ctgcgatatc aatccccttg ttgcccccct cagaagcgcc aaaggtttcg atcactttgc
     2101 cctcagtcgg ccagcgccag gtggagatag gcgtactggt tgatgtactg ctgacagtcg
     2161 gctcggttgt gcttgctgtt ggtaccgtta caggcactgt gaccgtggtc gcagttggct
     2221 tgttgttcgg caacattttg ttagcactct gttcacccga agactcagaa tacgtaattg
     2281 tcggttgcga cgcaacagca acggtggaat tttgtgcagg cttgatcaca actccttgct
     2341 ctgctgcgtc ggcctgggta atggcatttc cgcctgtgat tggcgtaccg gaagcattac
     2401 ccacctgtaa ggtctgaccg acgttcagcg cgtatggtgc ctgaatattg ttgcgctgag
     2461 caaggtcacg gaaatcgttg ccagtaatcc aggcgatata gaaaagcgtg tcgccttttt
     2521 tcacggtgta tgtactgccg ctataactgc ctttcggaat gttcccatac tgacggttgt
     2581 agacgattcg tccgttttcc atctgtaccg gctgctgagc tactggctgc acaggctgga
     2641 tttgcggttg ttgagtagcc tgaatttgtg gctgctgtac cggctgaatt tgcggttgct
     2701 gcgctgtaga cgtcgtcccc attttcggcg gcggcgtaat caacatacca gaattggtat
     2761 ttgcgggcgc attgccatta acggagctga ccggtgccgg tggatttgaa gtgtcagaac
     2821 agcctgccag ccatagcgaa accagtgaca atgccgcaat gcggcgaacg gtgaattttg
     2881 ggcttcccgc gctcatttat cccccaggaa aaattggtta ataaccagtg acataattac
     2941 cgtgcaaggc accatactga acactggaaa agatgttcac gatacgctga cttgcggcaa
     3001 aataaccagg aaaaatccag gtatttcctc acgttttaag ccagctcacc cttcactaaa
     3061 gggacaaagc gcacggcctc cacggtatcg ataataaatt cgcctccccg acgacgcacc
     3121 cgtttcaaat actggtgctc ctcccctacg ggtaagacga gaatcccgcc ttcgtccagc
     3181 tgcgtcatta gcgcagttgg aatttcaggc ggtgccgccg taacaatgat agcgtcaaac
     3241 ggcgcgcgtg cctgccaacc ttgccatcca tcgccatgac gggttgaaac attatgtaaa
     3301 tcaagatttt tcaggcggcg acgcgcctgc cactgcaagc ctttaatccg ttcaaccgag
     3361 caaacatgct ggacaagatg cgccaggatt gcagtttgat atcccgaacc agtgccaatt
     3421 tccagcaccc gcgactgcgg cgttagctcg agtaattcgg tcattcgcgc caccatatac
     3481 ggctgcgaaa ttgtctgccc ctgacctatc ggcaaggcga tattgtccca ggctttttgt
     3541 tcaaacgctt catcaacgaa tttttcacgc ggcacggcgg caagtgcatt cagcacctgc
     3601 tcatcctgaa taccttgcgc acgtaattga tccagaagtg cttgtacgcg tctgcttacc
     3661 attgcgtgcc aactcccacg ctgtttaacc agtctgaaac cacatcttgc gcgctatgcg
     3721 cagttaaatc cacatgcagc ggcgtgatgg aaacatagcc ctcatctacc gcagcaaaat
     3781 cggtccccgg accggcatca catttaccgc ccggcgggcc aatccagtac agcgtattgc
     3841 cgcgcggatc ttgctgcggg atcacctgat ctgccggatg tcgtgtaccg cagcgcgtca
     3901 cgcgaatacc tttgatttga tccaatggta aatccggaac gttaatatta agaatacgcc
     3961 cggtgcgcag cggctcttta cacaatgcgc gcaaaattga gcaggttacc gccgcggcag
     4021 tgtcgtaatg tttatgcccg tcaagcgaga cggcaagcgc cggaaaacct aaatgacggc
     4081 cttccatcgc ggccgctacc gtaccggaat aaataacatc atcccccaga ttcggcccgg
     4141 cgttaattcc ggacacaaca atgtccgggc gcgggcgcat cagagcattt acgccaagat
     4201 agacgcaatc ggtcggggtt cccatttgca cagcaatatc accattttca aaggtaaacg
     4261 tgcgcaggga agattccagt gtcagagaat tagaagcgcc gctgcggtta cgatcggggg
     4321 cgaccacctg aacgtcagca aactcacgca aggctttcgc cagcgtttgt ataccgggtg
     4381 catgtacccc gtcatcatta ctcagcaata tgcgcataat cacctgttgt gttgataagt
     4441 tccctgacaa cgctggttgc aaaacttccc gccggaagcc agaaacggat ttctacggtg
     4501 acgtcatccc accagttcca gcttaattgt tgcggataca gcagcatcgc tctgcgcgcg
     4561 gcttcaactt tttcacgcac cagtaaagtt tgtaattcag tttcttcggc gacagctgct
     4621 tgttcgaatg ccagcgcttc acgctgagtt ccccattcgc cactgcccag caatgcggcg
     4681 gttatcatta gctctttatc gttgacgcga cgctgtaatt ccgccagttc ttcggtggtt
     4741 gcaacaaacc agctaccacg tccggctaat tgtagcgcat cgccgtcaac aacttgatta
     4801 acgtctgctt ttttgaggcg ctcagcaaca atctgattaa acaacgcact gcgggctgcc
     4861 gacaaccaaa aactccgttt attgcgatcg cgcaccggag tattggtttg cgcccagcgc
     4921 agcgcgccct gcaagttgct accgccaatc ccaaaacgtt gggcaccgaa gtagttcggt
     4981 acacctttta cgcaaatatc gatcagacgt tgttcaacgt catcgcgatt gctcacttcg
     5041 cgcagaacca gggtaaaggc gttacctttc agcgcgccta aacgcagctt gcgcttgtgc
     5101 cgcgcatact ccagcacctg gcagccttcc agttgaaagg cgctcagatc gggcatctct
     5161 ttgcccggca cgcgagcgca taaccactgt tccgtaacag catgtttatc tttttgccca
     5221 gcgaagctga cttcacgggc atgaattttc aggaatttcg ccagtgcatc cgccacaaaa
     5281 cgggtattgc agccattttt gaggattcta accagaatat gctcaccttc accatcaggc
     5341 tcaaagccca aatcttccac caccacaaag tcttccggat tggctttcag cagcccggtg
     5401 ccttgcggtt taccgtggag gtaagtgaga ttatcaaact caatcatttt gttgccttaa
     5461 tgagtagcgc caccgcttca caggcaatcc cttccccacg tccggtaaat cccagttttt
     5521 ccgtagtagt ggctttcacg ttaacatcat ccatatggca gccgagatct tcggcaataa
     5581 acacgcgcat ttgtggaatg tgcggcaaca tcttcggtgc ctgagcgatg atagtgacat
     5641 cgacgttgcc gagggcataa cctttcgcct gaatacgacg ccaggcttcg cgcaacagct
     5701 cgcggctgtc ggcaccttta aatgccggat cggtatccgg gaacagcttg cctatatctc
     5761 ccagcgccgc cgcgccaagc aatgcatcgg tcaacgcatg cagcgccacg tcgccatcag
     5821 aatgcgccag cagccctttt tcgtaaggaa tgcgtacgcc accaatgata attgggcctt
     5881 caccgccaaa ggcgtgtacg tcaaaaccgt gtccaattcg cattatgtat tctcctgatg
     5941 gatggttcgg gtgaggtaaa attcggccag cgccaaatcc tccgggcgcg ttaccttgat
     6001 gttatccgcg cggccttcaa caagttgcgg atgaaaaccg caatattcca gcgccgaggc
     6061 ctcatcggta atggtagcgc cttcatttag agcgcgcgtc aggcagtcat gtaacagctc
     6121 acgagggaaa aattgcggcg tcagcgcgtg ccataagccg ttgcgatcaa cggtatgagc
     6181 aatggcattt ttgcccggtt cggcacgttt catcgtatcg cgcaccggcg cagcgaggat
     6241 ccctcccgtg cggctggttt cgctcaacgc caacaatcgc gcgaggtcat cctgatgcag
     6301 acaaggacga gcggcgtcat gcaccaatac ccactgcgcg tcgccagcgg ctttcagacc
     6361 tgccagcacg gaatcggcac gctcttcacc accatcaaca accgtgattc gtggatgatt
     6421 cgccagagga agttgtgcaa agcggctatc gccaggactt atggcaatga cgacacgttt
     6481 cacccgggga tgcgccagca gcgcatgcac cgagtgttca agaatggttt gattaccgat
     6541 tgagagatat tgcttaggac attccgtttg cattcggcgg ccaaatccgg ccgccggaac
     6601 cacggcgcaa acatccaaat gagtggttgc catgttaatt cccgggctga tttatcgatt
     6661 gttttgcccc gcagactgtg cgcgctttga cgcgtcaggc accagacgat aaaacgtttc
     6721 gcccggcctg gtcatgctga gttcattacg cgcacgctct tcgagcgcct cctggccgcc
     6781 attgagatcg tcaatttcgg caaaaagctg atcgtttcgc gctttaagtt tcgcgtttgt
     6841 agcttgctgt gccgccacat cattattgac gcgagtatag tcatgtatac cgttcttacc
     6901 gaaccacagc gaatactgta gccagaccag aatagccagc aacagcagcg ttagtttacc
     6961 catcctgccc cctgaaaaac ggcatcttca tcccatgcat ccgaagacga ctctacatcc
     7021 tctgttgggg ataccgcgac aacgcgggca aatgtaccac atttgtccat tgttacgtat
     7081 acccagggcg tgcagaaaat aatctcatta ttagttacgg attgaattat gaacagaata
     7141 gacgggaaag aacaattaac ccaatagcca cataaacagt gcaccaaaca taatgccaac
     7201 cgtcgccaga gtgaaaacaa tactgtagcg tagctttccg tccatcaatg aatgcagtgc
     7261 aatccccacc actacagcga cgggcatcag cgccagaaag aaaggccagg tgtagataaa
     7321 aaagagcagc gtgttagagc cataaatcaa catcggaatt gccaacgcgc acagccacga
     7381 taaaaaacca accacagcgc ctggcagcga ccatgtggtg tcttcaacct cagtaaggct
     7441 gtcgttattt gttaatgtaa tgttgtggct gttacgcata tctgatcctg ttactttgac
     7501 gaaccgggca tgaaacccgg tggtgtctca ggatctgata atatcgttct gtctcaacag
     7561 atctaataat tgctgtacca aatttgttac taattgttca ccattgagat gaatttctgc
     7621 cgattcaggc gcttcgtaaa cggaatctat tcccgtaaag ttgcgcagtt caccggcacg
     7681 cgctttctta tataaacctt ttggatcgcg ggcttcgcaa atcgccagcg gcgtatcgac
     7741 aaacacttcg ataaagcgcc cttctcctac gcgttcgcga accatctggc gttcggcgcg
     7801 gtgtggcgag ataaatgcgg tcagcaccac cagtccggct tcaaccatca aattcgccac
     7861 ttcaccgacg cgacggatat tctctttacg gtcggcatcg ctaaaaccga gatcgctgca
     7921 taatccgtgg cgaacgttgt cgccatccag cagatacgta ctgacgccaa gtttatgtaa
     7981 cgcctcctcc agcgcccccg cgacggttga tttaccggaa ccagagaggc cggtaaacca
     8041 cagcacaaca ccacgatgac cgtggtgctg ttcgcgttgt tgcagagtta ctgggtggct
     8101 atgccagacg acgttttcgt catgcagagc cattatttat ctcccagtaa atcgcgcgca
     8161 ccccaatgcg ggaaatggcg gcgaaccagg gcattcaatt ccagttcgaa tgcactgaat
     8221 tcagatggcg cagcagttgc ctggctaact ggttcgtgca ccataccggc acctacggtc
     8281 acattactca ggcgatcgat aaaaatcagc ccaccggtaa ccgggttttg ctgataacga
     8341 tctaacacca gtggctcgtc aaaggtgaga tctaccaggc cgatgccgtt cagcggcagg
     8401 ttttcaactt cgcgttgggt aaggttattg atatcaacct gataacgaat gccatcaaca
     8461 cgagcacgcg tcttcttacc ggcaattttg atgtcgtaac tctggcccgg ggaaagcggc
     8521 tgttccgcca tccataccac atccaccgac gcgctctgca cagccggtaa cacttcgtct
     8581 gccgccagca gcagatcgcc acggctgatg tcgatctcat ccgtcagcac cagggtgatc
     8641 gcttctccgg caaaggcttc ttcgcgatcg ccatcaaagg tcacgatccg cgcgacgttt
     8701 gattccacac cagagggcag cactttgaca cgttgcccga cttccacacg accggatgcc
     8761 agcgttccgg cgtagccacg aaaatcgaga ttcggacggt taacgtactg caccgggaag
     8821 cgcattggct gagcatccac cactcgctgg atctccacgg tttccagcac ttcgagcagt
     8881 gtcggaccgc tgtaccacgg catactttca ctttgcgaag ccacgttgtc gccttccagt
     8941 gcggagagcg gcacaaagcg gatatccaga ttacccggca gctgcccggc aaaggtcaga
     9001 taatcttcac gaatacgggt gaacgtctct tcactgtaat ccaccagatc cattttgttg
     9061 atcgccacga ccagatgttt gatccccaac agtgtggaga taaaactgtg acgacgggtt
     9121 tgatcgagca cgcctttacg ggcatcgatc agtaagatcg ccagttcaca tgtcgatgcg
     9181 ccagtcgcca tattgcgggt gtactgctcg tgccctgggg tgtcggcgat aataaattta
     9241 cgcttctcgg tagagaaata gcggtaagcc acgtcaatgg tgatgccctg ttcgcgctca
     9301 gcttgcaggc cgtccaccag cagtgccaga tccagctttt cgccctgggt gccgtgacgc
     9361 ttactgtcgt tatgcagcga tgagagctga tcttcgtaga tttggcgggt atcgtgtagc
     9421 aggcgaccaa tcagggtact tttgccgtca tcgacgctac cacaggtcag aaaacgcagc
     9481 aggcttttat gttgttgcgc aatcatccag gcttcgacgc cgccttcatt ggcgatttgt
     9541 tgtgcaagtg cggtgttcat cttaaaaata cccctgacgt tttttcagct ccatagaccc
     9601 cgcctggtcg cggtcaatta cgcggccctg acgttcactg gtggtggaaa ccagcatctc
     9661 ttcgatgatc tccggcagtg tttgtgcgtt tgactccacc gcgccggtca gcggccagca
     9721 gcccagcgta cggaaacgca ccatccgttt tttaatcact tcgcccggtt gcaggtcgat
     9781 acggttgtca tcaatcatca tcaacatacc gtcgcgttcc agaaccggac gttccgcagc
     9841 gagatacagt ggaacaatgt caatattttc cagccagatg tattgccaga tatcctgctc
     9901 ggtccagtta gagagcggga agacgcggat gctttcgcct ttgttaattt gcccgttgta
     9961 gttgtgccac agctccgggc gctgattttt cggatcccag cgatggaagc ggtcacggaa
    10021 agagtagatt cgctctttag cgcgggattt ctcttcgtcg cggcgcgcac caccgaaggc
    10081 ggcatcaaaa ccgtatttgt tcagcgcctg tttcaggcct tcagttttca taatatcggt
    10141 atgtttcgcg ctgccatgaa cgaatggatt aatccccatc gctacgcctt ccgggttttt
    10201 atgcaccagc agctcgcagc cgtaggcttt agccgtacga tcgcggaact catacatctc
    10261 gcggaatttc cagccggtat cgacatgcag caacgggaaa ggcagcgtac ctggataaaa
    10321 cgccttgcgc gccagatgca gcatgacgct ggaatcttta ccgatagagt agagcatcac
    10381 cggatttgag aattctgccg ccacctcgcg aataatgtgg atgctttccg cctccagttg
    10441 ccgcaggtgg gtaagtcgtt tttgatccat aaccgttcct ttgcaatacc gctattttct
    10501 tgccatcaga tgtttcgact atagggagcg taagagaacg aatgaaatta ccaattagaa
    10561 tgagtagttc cttaacggaa taacgatttg gcaaagctaa tatcaaaaag tgcttaaggc
    10621 accggatttc gggcgtttag gaagatttga aattgtttta gcgcagcggc agtttcatac
    10681 tatggcggta aaaaaatttg catggtattt aaggactcac tatgttttcc gcattgcgcc
    10741 accgtaccgc tgccctggcg ctcggcgtat gctttattct ccccgtacac gcctcgtcac
    10801 ctaaacctgg cgattttgct aatacccagg cacgacatat tgctactttc tttccgggac
    10861 gcatgaccgg aactcctgca gaaatgttat ctgccgatta tattcgccaa cagtttcagc
    10921 aaatgggtta tcgcagtgat attcggacat ttaatagtcg gtatatttat accgcccgcg
    10981 ataatcgcaa gagctggcat aacgtgacgg gaagtacggt gattgccgct catgaaggca
    11041 aaacgccgca gcagatcatc attatggcgc atctggatac ttacgccccg ctgagcgatg
    11101 ctgacgccga tgccaatctc ggcgggctga cgttacaagg aatggatgat aacgccgcag
    11161 gtttaggtgt catgctggaa ctggccgagc gcctgaaaaa tacgcctacc gagtatggta
    11221 ttcgatttgt ggcgaccagc ggcgaagagg aaggaaaatt aggcgctgag aatttactca
    11281 agcggatgag tgacaccgaa aagaaaaata cgctgctggt gattaatctc gataacttaa
    11341 ttgttggcga taaattgtat ttcaacagcg gtgtaaaaac ccctgaagca gtaaggaaat
    11401 taacgcgcga ccgggcgctg gcaattgccc gcagccatgg aattgccgcc accacaaatc
    11461 caggtttgaa taaaaactat ccgaaaggca ccgggtgttg taatgacgca gaaatattcg
    11521 acaaagccgg tattgctgta ctttcggtgg aagcgactaa ctggaatctt ggcaataagg
    11581 atggttatca gcaacgagca aaaacagccg cattccctgc gggaaatagc tggcatgacg
    11641 taagactgga taatcaacag catattgata aggcacttcc tggaagaata gaacgtcgct
    11701 gccgtgatgt tatgcggata atgctaccgt tggtgaagga gctggcgaag gcatcttaat
    11761 gggtttgaaa tgattactcc cttgcctgca ggaaatcaat gccaccagaa acagagtata
    11821 aaaaaggggt gcaggaggcc aaaccatcac aattcagtac gcggggtttt accccgcgtg
    11881 gtggctactt cttcgattcg taagcgagaa ctgccttaaa ctccatacca cgttctctga
    11941 tactcaactc acacaacgag tcgcctacca tcaacgtgaa tcccagtact gtacaacaca
    12001 gtacgatgat tgccacaagg gcatattttg tcagcatgtc ttgcctccct gcaaagaaga
    12061 gactaacatc cgaattgcta cgttcgaacg cgagcctcag gttgatagaa atatcgcctg
    12121 gggcttttgt ccatctggaa ccttgcgaat gcttaacgcc agacagcctc aagcacccaa
    12181 tgccattcta tacctgttaa ttttccctgc gatgttcacc tttaaatgcc ggaatgctgc
    12241 tcgcaaaaaa taaaaacgcc tccatcattt ctgacagagg cgtttaattt atccggcaat
    12301 atttaccctt cgtgtaatcc acattccctt tttaagccaa agaaacgcgt ttcttcttcc
    12361 gccatgccgg gttcccattt acgggttgta tgggtatcgc ccaccgataa atatccttca
    12421 tcccataatg ggtgatattt caggccatgt ttttgcaggt actgataaat agttcggtta
    12481 tcccagtcga taatcggcag cactttaaat acgccacgct gaattgccag caccggtaaa
    12541 ttggcgcggc tgccggattg ttcacggcgc aggccagcaa accaggtttg cgcattcagt
    12601 tctttcagcg cccggttcat cggttcgact ttgttgatgt cattgtactt ttcaatgcct
    12661 tcaacgccct gctcccacag tttgccgtag cgtgcttcct gccaggcggc gctttcggta
    12721 gcacgataca ctttcaggtt gagcttgagt ttgtccgtta actcgtcaat aaagcggtag
    12781 gtttccggga acagatagcc ggtatcggtg aggatcactg gaatatccgg gcgaatctga
    12841 ttcaccagat gcaggctcac cgccgcctga atgccaaagc tggaagaaag tacatattca
    12901 ccgggcagat tatccagcgc ccaggctacg cggccttcag cgtccagttt ttccagttgg
    12961 gcgttagttt ccgccagcgc cagaatccgg tctacctttg gcagttcgtt cagggcgttt
    13021 agatcgagtt tggacataag ttcctcactg tttgccttgc ctgatgcaac gctcacacgt
    13081 cttatcaggc ctacaagacc gggctgatgg ttaatcccac aaatcgcgcg ccggatcgag
    13141 caccgggcga atgatgcccg cacgcaccgt aaagtcgccg aagccttcac ccgcttcgcg
    13201 ctctttcgcc cagcgcccta tcagttcatc aagcgacgcc aggatttccg gctcggtgat
    13261 gttttcttta tacatccgtg ggatacgtgt cccaatgcgg ttgccgccaa gatgcaggtt
    13321 gtagcgaccc ggcgctttcc ccaccagacc cacttccgcc agcatcgcgc gaccacaacc
    13381 gttcgggcag cctgttacac gcatcacgat atgctcatcg ctgacaccat gtttcgccat
    13441 taaattatcg atgttgtcga taaaagacgg caggaaacgc tccgcttccg ccatcgccag
    13501 cgggcaagtc gggaatgaca cgcaggccat tgagttttca cgctgcggcg tgacggcatt
    13561 cattaacccg ctttctttgg cgatcttctc gatcttcgct ttctcgcttt ccggcacgcc
    13621 ggcgatgatc agattctggt tcgccgtaat gcggaaatca cctttgtgga tcttcgcgat
    13681 ctccagcaag ccggttttca gcgggcgccc cggataatca agaattcgac cattttcgat
    13741 aaacagcgtc aggtgccagt tatcatcaat gcccttaacc cagccaatac gatcgccgcg
    13801 tccggtaaac tcatacgggc gaatcggctc gaatttgatc cccgcgcgac gttcaacttc
    13861 cgctttaaac gtctcaaccc ctacgcgctc cagcgtgtat ttggttttgg catttttacg
    13921 atcggttcgg ttaccccagt cacgctgagt tgtcacgacg gcttccgcca ccgccagcgt
    13981 atgctccagc ggcagatagc caaactcact cgccgtgcgg gcgtaggttt tcttgttgcc
    14041 gtgttcgatg gaaagcccac cgcccaccag caggttaaag cccaccagct tgccgttttc
    14101 ggcgatcgcc acgaagttca tgtcgttggc gtgcagatcg atatcgttct gcggcgggat
    14161 cactaccgtg gttttgaact tacgcggcag gtaggtctga ccaaggatcg gctcttcatc
    14221 agtagtggcg actttttcct gatcgagcca gatctccgca tacgcgcggg tacgaggcag
    14281 cagatgttca gaaattttct tcgcccattc atatgcttcc gcatgcagct gcgactcgta
    14341 agggttcgag gtgcagagta cgttacggtt catgtcgttg gcggtcgcca gcgcatcaag
    14401 accgaccgag tgcagcatct ggtgcaccgg tttgacgttc tttttcagaa tgccgtggaa
    14461 ctgaaacgtc tggcggttgg tcaggcgaat gctgccatag atggtgtttt caccggcaaa
    14521 tttgtcgatc gcctgccact gtttagtggt aatcacccca cccggcagac gacagcgaag
    14581 cagcatcgcg tggcgcggct ccagcttctg ttcagcacgt tcggcgcgaa tgtcgcggtc
    14641 atcctgctga tacatgccgt ggaagcggat cagcaggaag ttatcgccct taaagccgcc
    14701 ggtcagaccg tcgtttaaat cttccgcaat ggtgccgcgc aggtagttgc tttcaagctt
    14761 catgcgctcg gcgtctgtca gttttccttc gaccactaaa ggccctggat gtttttcgct
    14821 cattagtaga catctcgctg ataacggcgc tctacgcgca gctcacttaa aaattcatcc
    14881 gccgcttcgg tgtccatgcc accaaattcg gcaatcactt ccagaagtgc ctgctcaacg
    14941 tctttcgcca tgcgattagc gtcgccgcag acataaatgt gggcaccgtc attgatccag
    15001 cgccacagct ctgcgccctg ttcgcgcagt ttgtcttgta cgtaaatttt ttctttttga
    15061 tcgcgcgacc aggcgagatc gatgcgcgtc agcacgcctt ctttgacgta acgctgccat
    15121 tccacctggt agaggaaatc ttccgtaaag tgtgggttgc caaagaatag ccagttttta
    15181 cccggcgctt cgtcggcggc acgctgctgc ataaaggcac ggaacggcgc gatgccggtg
    15241 ccagggccaa tcatgatcac cggggtttcc ggattggttg gcaggcggaa gttgtcgtta
    15301 tgttcgataa atacgcggac ttcaccctct tcttccacac gatccgcgag gaagctggag
    15361 gcaccaccgg cacgggcgcg gccttccacg tcgtaacgca ccacaccaac ggtaacgtgc
    15421 acttcgttct cgacttccgc ctgcgaggag gcgatggaat acaggcgcgg ggtcagtggg
    15481 cgcagcagat taatcagcgc ttcggcatcc agttgcgccg gagagaaacg caccatgtcg
    15541 acaatcggcg tcgtcgcggc gtaatgctgt aacttcgctt tatcgcccac cagcggcagc
    15601 agcgtttcgc tgcgggtaag cgtggcatag ttctcaacaa tgttggcggt gttgacggtc
    15661 agttcgaagt gccactgtag cgcttcgttc agaggcaacg ttttgccctc gacggtgaca
    15721 ggttcatcgc ctttcagcca cagcagttcg acaagttctt tcaccagtgc cggatcgttt
    15781 tgataccaga cgcccagcgc gtcacccggc tggtaacgca ggtcagagtc acctaagtca
    15841 atttcaatgt gacgaacgtc tttttcagag ttacgcccgg taattttctg gttaacagag
    15901 aggctcgcca ccagcggcgc gtctttgcta tacgggctgg tgtggatttc atttaccgtg
    15961 ccagtagcga cggattgcga aggtgccgcg acaggcgcac gcgatttaag cgcatcaacc
    16021 acgcgggcgc gccactcgct ggcagcagcc tggtattcaa cgtcggcatc gacacggtcg
    16081 agcaggcgtt caccgcccag ttccgccagc ttactgtcaa aatctttccc ggactggcag
    16141 aaaaattcat aagagctatc gccgaggcta aacacggcaa acgcggtgtt ttccagcttt
    16201 ggcgcttttt tggagaacag gaacttatgc agcgcgacgg cttcttccgg cggttcccct
    16261 tccccttgcg ttgacgtcac cacgataagc agtttttcgc tggcgatttg tttgaattta
    16321 tagtcgcccg cgttcaccag cttaacgttc agttttgccg ctaataaatc atcgcgtaat
    16381 gcttcagcaa cccgacgcgc attgccagtt tgcgaggcgg agataatagt tatacccggc
    16441 atttctgcgg cgggcgctgg cgtcgctgca agcgcagcag gctgctggtt gagcacgccc
    16501 cagaaatagc cagaaaccca ggcaagctgg gtaggagtta aatcggtcgt ggccgcctga
    16561 aggcgtgcca gttgctccgg gttcaacgga agcaacgcgg aaggtgggac ctgtgtcgtc
    16621 atgcgtcgtt atgttccagt aagcaaagct gtttctgcgc cctgtcagcg cccataaaac
    16681 agaagagaag gtaaggttaa cggggtgaac ggtgtggatt aaagacggga tagcgataac
    16741 taataaccaa atcgactaac ctgttttagc aatagtcttt aacaacaaaa tagattaacc
    16801 aacccaatga aaaacaaatg aatattgccc atcaataaga taaatcagcg attttgcgca
    16861 acaaagtcgt tttagataat gcgaaaaaac agcctttccg gtactctacg gcggttttat
    16921 cgtcatgtag agaaattatg atgtccacca cgttatttaa agatttcacc ttcgaagccg
    16981 ctcaccgctt gccgcacgtc ccacaagggc ataaatgtgg tcgcctgcac gggcattcct
    17041 ttatggtgcg actggaaatt accggggaag tcgatccgca tacgggctgg attatcgatt
    17101 tcgctgaact gaaagcggcg tttaaaccaa cttatgagcg cctcgatcac cattatctca
    17161 atgatattcc aggtctggaa aacccaacca gcgaagtttt agcaaaatgg atttgggatc
    17221 aggttaaacc cgttgtgccg ctgttaagtg cggtgatggt aaaagaaacc tgcaccgcag
    17281 gttgtatcta tcgcggcgaa tgataagagt gtgtcggcgg tcaatttccc ttaagtaacg
    17341 ctatgttagg gtgttgtgtt ctggatatct ggggcatgac atggaagacg actgcgacat
    17401 tattattatc ggtgccggaa ttgctggcac cgcttgcgcg ttacgctgcg cgcgagcggg
    17461 tttatccgtt ttgttactgg aacgagcgga gattcccggc agcaaaaatc tttccggcgg
    17521 gcgtttatat acccatgcgc tcgcggaact cctcccgcaa tttcacctga ccgcgcccct
    17581 tgaacgacgc atcactcacg aaagtctttc tctgttaacg ccagatggcg caacgacgtt
    17641 ttccagctta cagcccggcg gtgaatcctg gagtgtatta cgtgcacgat tcgatccgtg
    17701 gctggttgcc gaagccgaaa aagaaggtgt cgaatgcatc cccggtgcga cggtagatgc
    17761 actgtatgaa gaaaacggca gggtgtgtgg tgtcatttgt ggtgacgata ttcttcacgc
    17821 ccgttatgtg gtgctggcag aaggtgccaa cagcgtcctg gcggaacgcc acgggttagt
    17881 gactcgtcct gctggcgaag cgatggcatt gggaatcaaa gaagtgctga cgctggaacc
    17941 atccgctatt gaagaacgtt ttcatctgga gaataacgaa ggcgcagcgt tgttgttcag
    18001 cggcgggatc tgtgatgact tacccggcgg cgcatttctt tatactaatc aacaaacgct
    18061 ctcgttaggg attgtttgcc cgctctcctc ccttacgcaa agtagtgttc cggcaagcga
    18121 gctgctgact cgctttaaaa cgcatccggc agtgcgcccg cttatcaaaa acacggaatt
    18181 actggagtat ggtgcgcatc tggtgccaga aggtggcctg cgcagtatgc cggtgcaata
    18241 cgccggtaac ggctggctgc tggtgggcga tgcgttgcgc agttgcgtca ataccggaat
    18301 ttccgtgcgc ggcatggata tggcgctaac tggcgcgcag gcggcggcac aaacgctgat
    18361 aagcgcctgc caacaccgcg agccgcaaaa tctgtttccg ctttatcatc acaacgtaga
    18421 gcgcagcctg ctgtgggatg ttctacagcg ttatcagcat gttccggcgc ttttgcaacg
    18481 tcctggctgg tatcgggtgt ggcctgggtt aatgcaggat atttcccgtg atttatggga
    18541 tcaggggaat acacctgttc caccgctgcg ccagttgctc tggcgtcatt tacgtcgtca
    18601 cggcctgtgg catctgacgg gcgatgttat caggagtctg cgatgtctgt agcccgtaat
    18661 ctctggcgcg ctgctgatgc gccgcacatt gttccgactg actccgttga gcgccagacg
    18721 gcacaacggt tgattaacgc ctgtccagca ggtctttttt cgctcacacc tgaaggtggc
    18781 ttacgtgttg attatcacgg ctgcctggag tgtggcacct gccgtttgct gtgcgacgaa
    18841 tcaacactac aacagtggcg ctatccgcct tccggattcg gcatcaccta ccgctttgga
    18901 taaataataa ggataattta tgcccctctt acacctgctc cgccagaatc cggtgattgc
    18961 tgccgttaaa gataatgcca gcctgcaact ggcaatcgac tctgaatgcc agtttatttc
    19021 cgtgttgtac ggcaatatct gcaccattag taatatcgtt aaaaagatta agaacgccgg
    19081 gatatatgct tttattcatg ttgatttgct ggaaggcgcg tcaaataaag aagtggtgat
    19141 tcagtttttg aaactggtaa ccgaggcgga cggaattatc agtaccaaag cctcaatgct
    19201 gaaagcagca agagcggaag gttttttctg tattcatcga ctgtttattg ttgattcgat
    19261 ttcgtttcac aacattgata agcaagttgc gcaatcgaat ccggattgta ttgagatcct
    19321 gccaggctgc atgcctaaag tgctgggctg ggtgacggag aaaatccgcc aaccgctgat
    19381 tgctggcggg ctggtgtgcg atgaagaaga tgcgcgtaat gcgattaacg cgggtgttgt
    19441 ggcgctttcc accacgaata ctggggtctg gacgttagcg aaaaaattgc tttgacggga
    19501 taatcgtaac aagttgaatt tggtgtgatt tgtaggctgc acgccacatc cgacattcag
    19561 cgcctgatgc gacgcttgac gcgtcttatc aggcctacaa gtccccagcc cagaaccatc
    19621 ggcaggatca tccggcagca cgttattggc aatctgcgtg aatgtttgtc accagtgcct
    19681 ccagcaccac tttccagtca tcaactacgc caacatctgc ctgtgaaaat accgctgcac
    19741 tggcgtcatg attaatcgcc accacaaact tgctattgcg tacgcccgcc attaacgctg
    19801 ctgcgccgga agcgccaaca acaatacaca cctcaggtgc cagcagatgt ccggaaatac
    19861 caatcacttt ttcggcatca acgccaccgt tcatcactcg tgcccgactg tagcccactt
    19921 cggctcccat cttttctgcc agcatagcga tttcctggtt atctgcctct ccgccctgcc
    19981 ccaccaccag cacccgtctg gcttcagcta atgggtcgcg gataacattt ttcagatttt
    20041 ctatgctaac cagccagtcg ggaagcacac ctgggacaat gttcagttgc tgcatcccag
    20101 agggcaacgt ggcgttttta tctgctcccg cctgacgtgc cagcgaaagg catagcgggc
    20161 gtttgtctgt ttgcagcgtc gccgtcaacg catttcccca gtgggatttg cgcacgctta
    20221 cggttggaat atctaacgaa gtcacctggc agatgcttgc accatgtaaa cgccaggcca
    20281 gccgggtcga cagttcatcg ccaaacgcgc ccggaggaaa aaggacaacg tccgcaggcg
    20341 ttcgttgcca ctgttccacc agcgcatcca gcacctgttc cgcaaccatc ggctgtggtt
    20401 ctatttgcca atgtgccagg gtacaaccgc tgaaatcctg cgccgccagc cagctcgcta
    20461 ttgcggcatg gtcctgattt attgtcacaa ttacgatatt catggctgca tcctttggcg
    20521 taaatagtcc tgccatagct tttgcgcttt ttctgctacc gtttgcccac caatcagcgt
    20581 cgctccgcgt cgttgctctg ctcttgccag ttgcagacac tgcatcgctg gcaattcgac
    20641 agcaacagtt ttacggataa ttgctgcttt ccccgcagcc attcgctggc gcattcccgg
    20701 aactggcaag gccacttcac cgcactgacg cacggcaatc accgcaggca agcgaacccg
    20761 gcaacagcgc accccatgtt cagtacgctg ttcgagggtg ataaacggtg cgtcgagagt
    20821 aaagcgctcc acctgggtga agcagggcca gaccagcatt tccgccagca aaaatggcgt
    20881 ttgcccattc tgcccttcgc tgctttggca gccggtaatt atcagatcca gcggattctg
    20941 acgctgccat tcggcaatat ggcgagcgac aaattccggc gcaaagcgca gatctgccgc
    21001 cgtctccagc aatacagctt cctcaaaccc aagagccatg agatagcgca gccagtgcaa
    21061 cgcccgttca tcccccatgc tcaacgcggt taaagacatc ggcgtaccgt tttttctctg
    21121 cgccaacagc agcgcagctg cggcctgttc atcagcaccg agtaaacttc gcagaagcga
    21181 aacatccggt ccgctattac cctgagccgc cgcctgccac tctttttccg ccagcattcc
    21241 ggcatccggt tcggctttaa acgctaacag aatgttcatt cgcacctcct acatttcccg
    21301 ccgccaccag cgggagtgct ttggtttctg gtgcccatag ccaggtcaca acaaaaccaa
    21361 ccaacaacac tgccgccagg agtaataagg tggcttgcat tccccactgc gccagcaccc
    21421 acggcagcag gccagtactt accgccgccc ccaaccgact catggcggtg gcaaaaccga
    21481 cgcccagcga gcgaatgtca gtaggaaaac tttccgcagg caaaatgccc accagattac
    21541 tgactgccga aatggtggtg ctgaagagaa caaaaagcag cagcgttaat gaactgccgg
    21601 aaggcaaaca ggccatcact accagcgttg ccgccagcag caaaaaactt cccagcaaaa
    21661 acttgcgatg tgccagcagg tgcgtcagaa ctaatcccag cagcgcgccc acaattaaca
    21721 acgcattaag catcagactg gcagtcagcg catcttccag tccaatagtc tgggcgatag
    21781 ttggcagcca ggtataaatc acaaaccatg ggattacgag gcagacaaag aagacgctgt
    21841 taaacgctgt gcgccgccag tagcgcgaag agaacaaggt tttgatgtgt ttatgggtcg
    21901 ccgttgccac ttcatcgccc agtaaaacat gcgggccaaa atagcgatga acgatagcgt
    21961 gagcttctgc aaaacgcccc tggcgcagta gccagcgtgg cgattccggc gtgccccaac
    22021 gtagcagcgt aatcagcaag gctggtaacg ccgctgatgc cagcagccag cgccaggcct
    22081 ccgggctttc ggaaataaag tgatgtccgg caatacttgc cagcacatag cctacggtcc
    22141 acaccacact gaatgcgccc agcaaaatac cgcgatggcg gcgcggggaa aattcagcca
    22201 gcaaggtgtg accgactgag taatcgcctc ccagaccaat gccgatcaaa atgcgtaatc
    22261 caataagatg ctctggcgtg gtggcaaaaa attgcaagaa cgaagcgagc gtaatcagca
    22321 taaaactgaa ggtgaagatt ttttgccgcc cgatatggtc ggagatccac cccaaaacca
    22381 ggctgccaag aaacaaacca agtaacgccg agccgccgat cattcccgcc ataaacggcg
    22441 tcagttgcat ggcgggcgta agctgaataa tggcataacc aatgacgccg agaacataac
    22501 cgtcggtcag gtgtgcgccg aaagtgagcg cagcaattcg acagtgaaaa cggttaagcg
    22561 gtaaatcatc cattcgcacc ggtgaagtgt tcatctttac cccttcattg tccggtaata
    22621 agaacggctt ctcgcctgag aagccgaaaa ggcaacgcac cgtcatcgcg gaacgctgcc
    22681 tgacacatta tttttcaatc ggatagatag tacccgtgtt cataatgcca ttaggatcga
    22741 actgcttttt cagcccttcc agcaacgccc acgcgctgcc gtgttccagt ttgctccagt
    22801 gaacgcgatg tttaccgata ccgtggtgat gcaccatcga accgccgagg cgaatggttt
    22861 cttcgcagat gattttgttg agcggattgt ggtacttgtc gatttcctct tccggcttac
    22921 agtcaacgac gttgtagtcg tagacgaagt acatgttggt gccgttctga tagctatgag
    22981 aggagtgacc gcccagcatg gtgatgtcgt cggcgtgcgg gaactcggta cgaatacggt
    23041 taataacgct ttcgtagatt tcgtgaatgc agctccagca gccggacact tcagtggtaa
    23101 agcccatgtt gccggttttg aggatctgca cacgttcggc agccacttta tccggtcccc
    23161 agttcaggtt gttaaaccag gtttcgatca gcttgctgtc cacgcgctgg cactgcgggt
    23221 agcgcgccac gatttcagca atcccttcac ccgtcgcctt cgcaatgcga gggttacctt
    23281 cagccataaa gatcagcacg catttgccgt cggcaaaatg ggtgaagtgt tgggtgccat
    23341 cttcagcatc atacaaacga gcaatcgacg gacgataccc ttccaccatg atttcacgca
    23401 ggatgttgaa gccggttttc atatcttcca ggatatagcc gtagaagaga ttgttttccg
    23461 gcgtaaattt gaagattttc actgtgactt cagtgatata gcacagtgca ccttcgttgc
    23521 cgatgatgat atgacgaatg tccgggccag ccgcgcgacg tggcacgttt ttaatgcgtg
    23581 tgacggtgcc atctgccagc accgcttcca gaccaacgac catatcttcg attgcgccgt
    23641 agagtgtgga gaactgcccg atactgcggg tcgccaccaa gccgcccatc tgcgccagcg
    23701 gctttgactg cggagaatgc cccgtggtgt aacctttttc gcgcaatgcg ttttccagca
    23761 cctccagcgg aacgccacat tgcgccgtgg cctgcatatt ctcaatatca atattaatga
    23821 tttgattcat ggcggagccg tcgagcacca ccgagttttc cacaacggtt tccagtccac
    23881 cttcggtggc ggaagcaccg gtacgtggca caccgttaat tttgtgcgca ttcataaaat
    23941 tcagcacacg ggatacttgt tctgtggaac cgagttttac gaccgctgcc ggaatcggca
    24001 aagtataaat tccatgaata tccggaaatt tacgaaaacg gtcaatactg tttttcttta
    24061 ataccgtttc atcggtaata acgcgatctg caccaacaat ttcctttagc tggtcgacaa
    24121 tcgctacgcg agataaagac ataataattc cttcctgata ataaaaatgg aatagttatt
    24181 aataagagaa aacagaaacc accgttatga attagcgcac taaataaccg ccatcaacca
    24241 ctaataaatg tccgttgaca taattcgatg ccggacttgc gaggaatacg gctgccccca
    24301 ttaaatcctg agtatcaccc caacggtttg ccggaatatg atcaagaaca cgctggttag
    24361 tttctggatt actacgggtc gccagcgtaa tatcggttgc ataataacca ggagcaatac
    24421 cattcacctg aatattatat tggcctaatt catcgcaata ggctttggtg aatccggcaa
    24481 gagcatgttt agtggcagaa taagcaggtg accattgacc acctaagtaa gagaacaatg
    24541 aacagatatt aatgatttta ccgcttttct gcgggatcat aatttttgcc gcttcgtagc
    24601 ttaactcgaa tgcggcggtc aggttcacgt caatcatcgg gtcccagtcg gcacgaccaa
    24661 agtccagcac cttattcagc ttacaaatac cggcattatt gaccagaata tcgacggtac
    24721 cgaagcgctc acagcaggca gcgataatct tctgcggcgc gccttctgcg gtgatatcca
    24781 cctgcataaa atcaacttca acgccctgtt tttcaatcat ttcctttgtt tcgccgttat
    24841 ctttgacgaa actaggaata aagacatttg cgccagcttt ggccaacgcc atggcaaatg
    24901 cctggcctaa accgctattc ccgccggtaa caattgctgt tttacctttc agggagaaaa
    24961 aatccattga gaacgcattg agagattcga ttgacatagt tagctccaat ttccgtaagg
    25021 caaaaaaaaa gaaaggtaac ttcccccgcg agagggaagt tacctttctc attatctctg
    25081 gtaactggag ataattttta gcataaggac ataactgcta atgtgatcgt aatcacagtg
    25141 tgatgttcgt attgtcaaat aatttaaaaa agcagaagcg tgattactct cacacaacac
    25201 ctctgctttc catgttattt cttattgcaa ttactagaga cctgagaaaa gtaatccacc
    25261 tgaaagggcg gcttactttt ctcttttttt tgtctattac aaatgggaat acgatatgca
    25321 acacaactca tatcgccgtt ggataaccct cgcgataatt agttttagcg gcggcgttag
    25381 tttcgacctg gcttatttac gttatattta tcaaattccc atggcaaaat ttatgggatt
    25441 cagcaatacc gagataggtt taataatgag tacctttggt attgcggcca ttattttcta
    25501 tgcccctagc ggcgttattg ccgataaatt ttcacatcgc aaaatgatta cttccgcgat
    25561 gatcattacc ggattactgg gtctgttaat ggcaacatat ccaccgctgt gggtaatgct
    25621 ctgtattcag gtcgcctttg cgataacgac gattttaatg ctgtggtcgg tgtcgattaa
    25681 agccgcatcg ttgctgggcg atcatagcga gcaagggaaa attatgggct ggatggaagg
    25741 gctgcgcggc gtcggtgtaa tgtcgctggc ggtgtttacc atgtgggtct tttctcgctt
    25801 tgcaccggat gacagcgcca gcctgaaaac ggtcattatt atctatagtg tggtttacat
    25861 tctgctgggc attttgtgtt ggttttttgt tagcgataac aacaacctac gcagtgccaa
    25921 taacgaagaa aaacagtcat tccagcttag cgacatcctg gccgttttgc gcatcagcac
    25981 tacctggtat tgcagcatgg tgatttttgg tgtcttcacc atctacgcca ttctgagtta
    26041 ctccaccaac tatctgaccg aaatgtacgg catgtcgctg gtggcggcga gctacatggg
    26101 gattgtgatc aacaaaatct tccgcgcgct gtgcggccca cttggcggca ttatcaccac
    26161 ctacagtaaa gtgaaatccc ctacccgcgt ggtccaaatc ctttccatca tcggtttgct
    26221 ggcgttgaca gccctgctcg tcacgaactc taacccgcaa tcggtcgcga tggggattgg
    26281 cttgatttta ttactgggat tcacctgtta cgcctcacgc gggctgtact gggcctgccc
    26341 tggcgaagcg agaacaccgt cttacattat gggcaccacg gtaggtattt gttcggtgat
    26401 tggattcctg ccggatgtct tcgtttaccc gattatcggc tactggcaag acaccctgcc
    26461 cgctgcagaa gcctatcgca acatgtggct aatgggcatg gcggcgcttg gcatggtgat
    26521 catctttacc tttttgctgt tccaaaaaat tcgtactgct gatagcgccc ccgcaatggc
    26581 tagcagcaag taagccagga aaattacagg agaaaaatca tgtcgaaaaa atacatcata
    26641 gggattgatg gcggaagtca gagcacaaaa gtggtgatgt acgatctgga aggtaatgtg
    26701 gtttgcgaag gcaaaggctt attgcagccg atgcacacgc cagatgccga taccgcagaa
    26761 catcctgacg atgatttatg ggcatcacta tgttttgcca gtcacgattt gatgagccag
    26821 tttgccggga ataaagaaga tattgtcggc attggcctgg gctcaatccg ttgctgccgt
    26881 gcgctattga aagccgatgg tacgccagcc gcgccgttga ttagctggca ggatgcacgc
    26941 gttacacgcc cttacgaaca cactaatcct gacgtggcct atgtcacctc tttttcgggt
    27001 tatctgacac atcgcttaac cggcgagttt aaagacaata tcgccaacta ttttggtcag
    27061 tggccggtgg attataagag ctgggcatgg agcgaagatg ctacggtaat ggaaaagttt
    27121 aatatccccc gccagatgct ttttgacgtg caaatgccgg gcaccattct tgggcatatc
    27181 acaccacaag ctgcgctggc gacacatttc ccggctgggc tgccggttgt ttgtaccacc
    27241 agtgataaac cggtagaagc tctgggagcc gggttactgg atgatgaaac ggcggtaatt
    27301 tctcttggca cctatatcgc gttgatgatg aacggcaaag cactgccgaa agatccggtg
    27361 gcgtactggc cgattatgtc ttctattccg caaacattgc tgtatgaagg ttacggtatt
    27421 cgcaaaggca tgtggacggt gagctggctg cgcgacatgt taggcgagtc gttaattcag
    27481 gatgccaaag cacaggatct ttcaccggaa gatttgctca acaaaaaagc gtcttgcgtg
    27541 ccgcctggct gtaatggtct gatgacggtg ctggactggc tgaccaatcc gtgggaaccg
    27601 tacaaacgcg ggattatgat cggttttgat tccagcatgg attacgcatg gatatatcgt
    27661 tcgatactgg aaagcgtggc gctgacgctg aagaataatt acgacaatat gtgtcatgaa
    27721 atgaatcact ttgcaaagca tgtgatcatt actggcggcg gttcgaacag cgatctgttt
    27781 atgcagattt ttgccgacgt gttcaacctt ccggcacgcc gaaacgccat taacggttgt
    27841 gcaagtctgg gggctgcgat caatacggcg gtaggtctgg ggctataccc ggattacaca
    27901 acggctgtcg ataaaatggt tcgcgtgaaa gatatcttca taccgattga gagcaatgcc
    27961 aaacgctacg acgcgatgaa taaaggcatt ttcaaagacc taaccaaaca tactgatgtg
    28021 atcctgaaaa aatcgtatga agtgatgcat ggggaattgg ggaatgcgga ttcgatccag
    28081 agctggtcga atgcgtaagt gagataaaca gtgccgggta gatgattaat cccggcatat
    28141 tcacgtttcc actacaaaat accgggtaac cactgctgaa tattgttctc tcctgagcag
    28201 cgtttatgct ttaattgcta aaccgattta attagtttag caattagtat attggagcct
    28261 ttatgagcac aagagtatgg gtactcggtg atgcggttgt tgatttatta cccgaaagcc
    28321 aggggagact actacagtgt cctggtgggg cgcctgctaa tgttgcagtc ggtatcgcaa
    28381 ggctgggggg gaaaagtgcc tttattggca aagttggcga tgatcctttc ggtcgcttta
    28441 tgtatcaaac actgagtaca gaaaatgttg atacacgtta tatgtctctt gatcctcaac
    28501 atcgcacctc aattgtggct gtaggacttg atgagcaagg agaaagaaac tttaccttta
    28561 tggtacgccc aagtgccgat ctttttttac aacctgatga ccttcctgca tttgggccgg
    28621 gtgaatggct ccatctttgt tccattgcgc tcagtgcaga accttcccga agtaccgcat
    28681 ttctggctat ggagaaaata cgtcaggctg gcggaaacat cagttttgat cccaatatcc
    28741 gcagcgatct ctggcagagt gaagcgctgt taaggaaata ccttgatcgt gcgctttcgc
    28801 tggcgaatat cgctaaattg tccgaagaag agttgttatt catcagtgac gaaagccagg
    28861 ttcagcaagg cgcatattca ttagtacaac gttatccgtt gactttattg cttattacac
    28921 aaggaaaaaa tggcgtactt gtgtattttc aggggcagtt tatccactat cccgccaaac
    28981 ctgtttctgt cgtcgatacg accggggcag gagatgcttt tgtcgctgga ttacttgccg
    29041 gtctggctga ttctggaata ccaacaaata ccagacagct tgaacgaatc attgcacaag
    29101 ctcagatttg tggtgctctg gcgaccacgg ctaaaggcgc gatgaccgcc ttaccccgac
    29161 aacacgatct cccttcacaa tagtgatcct gctgttgtat ctgtaacccg atttgtcgtg
    29221 cctgtcacat ttactaaatc ggtttagcga aaataattgc tctggctaaa aagtcagctt
    29281 tattatcaac acctaaaccg gtttagcatt ttagcaattt ttataattct ctgatgggac
    29341 gcgacaaatt atgtataaaa aaacaacttt ggcagtgtta attgctttgc tgaccggtgc
    29401 tacaacggta catgcgcaaa cggatattag cagtatcgaa tctcgactgg cggcgttgga
    29461 acaacgttta aaaaatgcgg aatcccgcgc ccaggcggca gaagcaaggg ccaaaacagc
    29521 tgaattacag gttcagaaac tggctgaaac acaacaacaa aatcagctaa caactcaaga
    29581 agtagcacag agaacagttc agctcgaaca gaaatccgca gaaaacagtg gttttgagtt
    29641 tcatggctat gcccgttccg ggttactgat gaatgatgcc gcttccagta gcaaaagtgg
    29701 gccgtatctg actcccgcag gtgaaactgg tggagctgtt ggccgtctgg gaaatgaagc
    29761 cgatacctat gtcgagttaa atgtagaaca taaacaaaca ctggataacg gtgcgaccac
    29821 acgctttaaa gcaatgttgg ctgacggaca aagagattac aacgactgga ctggcggctc
    29881 cagtaacctg aatatccgac aggcttttgc cgaactgggc gcattaccaa gttttaccgg
    29941 agcattcaaa gacagtactg tctgggctgg taaacgcttt gatcgcgaca attttgatat
    30001 tcactggtta gactccgatg tcgtattttt agcgggaacg ggcggcggta tctatgacgt
    30061 aaaatggaac gagacattcc gcagtaactt ttctctctac ggacgtaatt tcggcgatct
    30121 tgatgatatc gacaataacg ttcagaacta catcctcacc atgaatcatt atgcaggccc
    30181 cttccagttg atggttagcg gattacgggc aaaagataat gatgatcgaa aagatgccaa
    30241 tggtgatctc attcaaactg atgctgcaaa tactggcgta catgcgttag ttggtctgca
    30301 caatgacact ttctatggcc tgcgtgaagg gacggcaaaa acagcactgc tatatggcca
    30361 tggcctgggt gcggaagtca aagggattgg ctccgatggc gctctgctgt ctgaggcgaa
    30421 tacctggcgc ttcgcatctt acggcacaac acctctggga agcggttggt atgttgcgcc
    30481 agcaattctc gcacaaagca gtaaagatcg ttacgtcaaa ggcgatagct acgaatgggt
    30541 gaccttcaat acacgtctga tcaaagaggt aacacagaat tttgctctgg cctttgaggg
    30601 tagctatcaa tatatggatc tgaagccaaa ggggtatcaa aaccacaacg ccgtaaacgg
    30661 cagcttctat aaactcacct ttgctccaac tctaaaagct aacgatatca ataatttctt
    30721 tagccgtccg gagcttcgcc tgtttgccac ctggatggac tggagcagca aacttgatga
    30781 ttttgccagc aatgatgctt tcggcagcag tggtttcaat actggcggag agtggaattt
    30841 tggtgtccaa atggaaacct ggttttaata ccagacagcc tcacggatgt gaggctttta
    30901 ctgaaataac aatgaattag agggttatat ggatttcgat aaaatcgccc aatcgttgct
    30961 tcctttatta ggtggtaagg agaacatcgc cagtgcagca cattgcgcca ctcgcctacg
    31021 gctggtactg gttgacgaca cacttgccga tcaacatgcc attggccaga ttgatggagt
    31081 gaaaggttgt tttcgtaatt cagggcaaat gcaaatcatc tttggcaccg gcgtggttaa
    31141 taaagtctat gctgccttta ttcaggttgc aggtattagc gaatccagta aagcagatac
    31201 cgctcgactc gccgctcaaa aactcaatcc ttttcagcga atagcacggc tactttctaa
    31261 tatttttgtc cccatcattc cagccattgt tgcttctggt ttattaatgg ggcttctggg
    31321 aatggtgaaa acatatggct gggtgaatgc cgataatgcg atttatatcc tgctggatat
    31381 gtgcagttca gccgcattta ttattctccc catccttatt ggctttactg ctgctcgtga
    31441 atttggtgga aatccttatc ttggcgcgac attaggaggg attctgactc acccggcact
    31501 cacaaatgcc tggggcgttg ctgcgggatt tcagacaatg aacttcttcg gttttgaaat
    31561 cgccatgatt ggctatcagg gaacggtttt ccccgtgctt ctggcagtat ggtttatgag
    31621 catagtggaa aaacagttac gcaggtttat ccctgatgct ctggatctca ttctgacgcc
    31681 atttctgact gtcgtcattt ctggctttat cgctcttttg attattggtc cggcaggacg
    31741 agcgttaggt gacggtatct ctttcgttct cagcacgctg attgcacacg caggctggtt
    31801 agcagggttg ttattcggcg ggctatattc agcgattgtt attacgggta ttcatcatag
    31861 cttccacgca attgaagcag gacttttagg taaccctgca atcggtgtta atttcctgct
    31921 gcctatttgg gcaatggcaa acgttgcgca aggcggtgca tgtctggcgg tatggtttaa
    31981 aaccaaagat acaaaaatta aagcaatcac cctaccctcg gccttttccg caatgttagg
    32041 gattactgaa gccgctattt ttggtatcaa ccttcgtttt gttaaacctt ttattgcggc
    32101 cttaattggt ggcgctgccg gcggtgcctg ggttgtctct gttcacgttt atatgactgc
    32161 cgttggtctg accgcaatac ccggcatggc aatcgttcag ccaacatcgt tggttaatta
    32221 tattattgga atggtgattg ctttcgctgt cgcttttagc ctctctttat tactcaaata
    32281 caaaacggac gaggagtaat gtcgatgcct tcacaaatgc ccgcgatttt acaggcagtg
    32341 atgaaaggtc agtccaaagc actggcagat gcccactatc cttgttggca tctcgctcca
    32401 gtcaccggac taatgaacga tccaaatggc ttttgttggt ctggaggccg ctatcatctg
    32461 ttttatcaat ggaatccact ggcttgtgat cataagtaca aatgttgggg gcactggagt
    32521 tcgacggatt tattgcactg gcagcatgaa ccattagcgc taatgcccga taaagagtac
    32581 gaccgtaatg gttgttactc aggaagcgcg gttaataatc agggcgttct cacactttgc
    32641 tataccggta atgttaagtt tgatgatggc agcagaacag catggcaatg tctggccacc
    32701 gaaaataacc agggcgggtt tgataaacta ggccctgtca taccgttacc tgatggttac
    32761 accggacatg ttcgtgatcc aaaagtctgg aagcacaaca gccaatggta catggtgctt
    32821 ggcgcgcaag ataaagagaa acgggggaaa gtactgctgt attcctctgt cgatctctat
    32881 acctggtctt ttcatggaga aattgctggt aatggtctga atgaaataga taacgcagga
    32941 tacatgtggg aatgtcctga tcttttcgcc cttgatggtg aatacattct cctctgctgc
    33001 ccccagggaa tggcgcgcga acatgagcgc tatctgaata cttatccctg cgcctggcta
    33061 catggacagt ttgattacga gacaggcaag tttacgcatg gcgctttctc agagctggat
    33121 gcgggatttg aattctatgc accacagacg atggaagccc cggacgggcg acgattgctg
    33181 gtcggctgga tgggggttcc tgatggcgaa gagatgttgc aaccaaccag aaagcatcat
    33241 tggcagcatc aaatgacctg ttttcgcgaa ctctcattcc agaagggaaa attgtttcag
    33301 atgccaatca gggaactcgc gcaactgcgt gaagctgaac acttctggca aggaaaagcg
    33361 gatcatgcgc cgcatgtcga aattgaacgc ctggaaatgg acatcattcc gtcgggtgag
    33421 ctgtatctga attttggcaa tgcgctggca ctgcatctta atgacgatgg tattcaacta
    33481 cagaggagaa gccttgcagg gcaagagaaa ctaactcgtt actggcgagg aagcgtaacg
    33541 tcactgaaga ttctctgtga cagttccagc gtagagatat tcatcaataa tggtgaaggt
    33601 gtaatgagta atcgttattt tccccatcat ccagcttcgc tgatattaca gggtgagtct
    33661 gacgtcacgt tacgctactg gtcgctacgc gcctgcatgg tagaatgagg gttttgaatt
    33721 acggatcctc gtcgtgagaa aaaccaaacg cgttactatt aaagacattg ctgaactggc
    33781 gggagtgtcg aaagcaaccg ccagtctggt tctcaatggt cgtggcaaag agttacgtgt
    33841 tgcgcaggaa acgcgtgagc gtgtgctgag tattgcccaa cagcagcatt atcagcccag
    33901 cattcatgcc cgctcattac gggacaaccg cagtcatacc attggtctcg tcgttcctga
    33961 aatcactaac tacggatttg cagtattttc ccatgaactg gaaacattgt gtcgggaagc
    34021 gggcgtccaa ctcctcatat cctgtactga tgaaaatcct tcacaggaaa caatggtggt
    34081 gaacaatatg attgcccgcc aggttgatgg gttaatcgtc gcatccagta tgctgcatga
    34141 taatgactat caaaaactta gcgagcaatt acctgttgtg ttgttcgatc gccacatgaa
    34201 cggcagcaca ttgccattgg tcattactga ctctgtttct cccaccgccg ctctggtggc
    34261 agatattgca cggagtcacc cggatgaatt ctattttctg gggggacaac cccgtttgtc
    34321 accgactcgt gatcgactgg aaggatttac acaaggcttg cagcaagccg gagtaacgct
    34381 acaacctgaa tggattattc acggtaatta tcatccgagc tcaggctatg aaatgtttgc
    34441 cgcgctatgc gctcgtttag gacgtccccc taaagcctta ttcactgccg cttgtggatt
    34501 actggaaggc gttctgcgct atatgagcca atataattta ctcgacagta aaatccacct
    34561 ggcgagtttt gatgatcatt acttgtatga ttcactttcg gtaagaatcg acacaataca
    34621 acaggataat cgccaacttg cttttcactg ctttgaactt atttcgcaac tgattgaagg
    34681 tgaaacgcca tccccattac agcgttatct tcctgccagt ctacaaaaac gctaccgata
    34741 atcccgtcac tatcttgcat cgcccctgta aaacggggcg attattttca tcctgaacgt
    34801 taaatatcaa tgatgcataa tcaaatcagg caatatttaa atatttatgc gtttgcatcg
    34861 acaaacgcca gttgcgcgca atgcaggtat cgatacataa acgcgtagca tcttccttct
    34921 ggctaattgg ctgcagtgca atgactcgcg gtttatcatc ggtcagcgtc gcaagcaatt
    34981 catccagtgc ttcgatatca cgtacgcgtc ccactgggtg tttgatttcg ttggctcgct
    35041 ccagtgcctg tgacaacact tcatagccac cgcgcatatt cagctttggc gacacggtga
    35101 cccaggtatt cggtgtacag cgtacttcat gagtaccact ggtttctatc tggcagctaa
    35161 aaccgttctt ttcgagcagg tcagtcagcg gcagcaaatc atgaatgcaa ggctcaccac
    35221 ccgtaatcac cacgtgacgc gcggtgtatc cctgacggcc aatgacagcc agcaaatctt
    35281 cactgctcgc cgccccccac ttatcactct ctttggtctt cgccagaatg ctgaaaaggg
    35341 agacttcctg atcctcaagc ttttcccagg tgtgtttggt gtcgcaccag gcacagccaa
    35401 ccggacatcc ctgtaaacga ataaaaatgg cggggacgcc ggtaaagtaa ccctcacctt
    35461 gcagggtctg gaacatctcg ttaatcgggt actgcatagc attctctgtg aagtggataa
    35521 ttgttaatta ttgcagatcc tgccacaaca atcatgtctt attaacattc tgttaccggc
    35581 aggtttaaag aaaaatgcga aacatatcgt taataataaa agggagtgac gtattatgtc
    35641 agaaagaaat aaagtaaagg gatttaatca tgtcaaaaac attcaccaaa attatattta
    35701 tcttttctgt attcattttt aattataacg aacctcaaat taccgatacc gccgtcaatt
    35761 tatttatcca gctttaatag gacatttata atgcgctatt tcatactaat attcacactc
    35821 gtgtgttcct ttgttgcagc ccaaccaact attgtcccac aattacagca acaagttacg
    35881 gatcttacga gtagcttaaa ctcacaagaa aagaaagaac tgacgcgcaa gctagaatct
    35941 attttcaata atacccaagt acaaattgct gtattaatta ttcccacaac caaagacgag
    36001 acaattgaac aatatgctgc aagggttttt gacaattggc gtttaggaga tgccaaacgc
    36061 aatgatggga tactgatcat tgttgcctgg tcggatcgca ctgtccgcat ccaggtaggt
    36121 tatggactgg aagaaaaggt aaccgatgct ctggctggag atatcatccg tagcaacatg
    36181 atacccgcat ttaaacaaca acaattagct aaaggattag agttagctat aatcgctttg
    36241 aataaccgac tcacttcaca acaccaatat tcggttaatc cttcagaaag tgaatcagca
    36301 tcttccagcg atcattacta ctttgctatt ttttgggtat ttgcagtgat gttcttccct
    36361 ttctggtttt ttcatcaagg cagtaatttt tgtcgcgcat gtaaaagcag tgtctgtatt
    36421 tcagcaatct atcttttaga tttattcctg ttctccgata aaactttttc cattgctgta
    36481 tttttcttct tttttacttt caccaccatt atggtcttta cctgtttatg tgtacttcag
    36541 aaaaaagcat ctggtagaag ttatcattca gacaacagcg gttccggtgg cggctcaagt
    36601 ggcggcggat ttagcggcgg gggcggttct tctggcggtg gcggcgcgtc cggtcgttgg
    36661 taactcaacc ataaaaaacg ccagcccgaa ggctggcatt tttaaatcag ataaagtcag
    36721 acttatgcct ggcctttgat ctctttacga ccgttgtacg gtgctttttc gcccagagct
    36781 tcttcgatac gaatcagctg gttgtattta gcaacacggt cagaacggct catagaaccc
    36841 gttttgatct ggcctgcagc agtaccaaca gccaggtcag cgatggtagc gtcttcagtt
    36901 tcgccagaac ggtgagagat aactgcagtg tagccagcgt ctttcgccat cttgattgca
    36961 gccagagttt cggtcagaga accgatctgg ttgaatttga tcaggatgga gttagcgata
    37021 cctttttcga taccttcttt caggatcttg gtgttggtta cgaacaggtc gtcaccaacc
    37081 agctggattt tgtcgcccag aactttggtt tggtatgcga aaccgtccca gtcagattcg
    37141 tccagaccgt cttcgatgga aacgatcgga tactgtttgg tcagttcttc caggaagtga
    37201 gtgaattctt cagaggtgaa tgctttgttg ccttcgccag ccagaacgta tttaccgtct
    37261 ttgtagaatt cagaagctgc gcagtccatc gccagagtga tgtctttgcc cagttcataa
    37321 ccagcagctt taacagcttc agcgataaca gccagagctt cagcgttgga acccaggttc
    37381 ggcgcatagc caccttcgtc accaacagca gtgttcatgc ctttcgcttt cagaactttt
    37441 gccaggtgat ggaaaacttc agaacccatg cggatggctt ctttcacagt tttcgcgcca
    37501 accggctgaa tcatgaattc ctggatatca acgttgttgt cagcgtgctc accaccgttg
    37561 atgatgttca tcatcggaac cggcatagag tatttgcccg gagtgccgtt cagttcagcg
    37621 atgtgctcgt acagcggcat acctttagca gctgcagcag ctttggcgtt agccagagat
    37681 acagccagga ttgcgtttgc gccgaatttg gatttgtttt cagtgccgtc caggtcgatc
    37741 atgatcttgt caatgccagc ctgatcttta gcatctttgc caatcagcgc ctgagcgatc
    37801 gggccgttaa ccgcagcaac agctttggtt acgcctttgc ccaggaaacg ggatttgtcg
    37861 ccatcgcgca gttccagagc ttcacgggaa ccagtagaag cacctgacgg agcagctgcc
    37921 ataccgacga aaccaccctc cagatgtact tcggcttcaa cagtcgggtt accacgggag
    37981 tcgatgattt cacgaccgat gatttttacg attttggaca ttaggttttc ctcaagtcac
    38041 tagttaaact gaaactccag acaaacaacg cgtacccagg gtacgcgttg ccgttctaac
    38101 ttttttactt acttcgcctg acgtttctgg aactcgctgg cggcttttac aaagccagca
    38161 aacagcgggt gaccatcacg tggagtagaa gtaaactccg gatggaactg gcaagccacg
    38221 aaccacggat gattcggaac ttcgatgatc tcgaccaact gatcatcccc ggaacggccc
    38281 gcaacgcgca gacctgcatc ttcaatctgt ttcaacagca tgttgttgac ttcgtaacgg
    38341 tgacgatgac gctcaacaat tgtcggcgca ttgtacagct ggcgaaccag gctatcgtca
    38401 accaactggc actgctgtgc gccgagacgc atggtaccgc cgagatcgct cttctcgcta
    38461 cgaacttcaa cgttgccgtt ttcatcgcgc cactcggtaa tcagcgccac aaccgggtac
    38521 ttacagtctg gcacaaattc cgtagagttg gcgttctcca tgttggcaac atggcgagcg
    38581 taatcaatta acgccacctg catacccagg caaatgccca gataaggaat attgttctca
    38641 cgcgcaaaac gcgcggtcgt aatcatgcct tctacgccac gatagccgaa accgccaggt
    38701 acgaggattg cgtccagacc tttgaggatt tcaacgccgc gcgtttcaac atcttgtgaa
    38761 tcgatcagtt tgatgttgac gctgacacga ttcttcagcc caccgtgttt cagtgcttcg
    38821 atcacggatt tgtatgcatc cggcagctca atgtatttgc cgaccatacc aatggtgact
    38881 tcacttaccg gattcgcttc ttcgaagata acctgttccc attcggacag attcgcttcc
    38941 gggcagttta agctgaatcg tttacaaata taatcgtcca gcccctgaga tttcaacagg
    39001 cccggaattt tatagatgga atcgacgtct ttcagagaaa taaccgcttt ttccggaaca
    39061 ttacagaaca atgcaatctt cgcacgttcg ttcgctggaa cggcgcgatc tgaacgacaa
    39121 atcaggatat caggctggat accgattgaa agcagctctt ttacagagtg ctgagtcggt
    39181 ttggttttga cttcacctga cgctgccatg tacggcacca gcgtcaggtg cataaacagg
    39241 gtgtgctcac ggccaatttc aacagccatc tggcgaatcg cttcgaggaa cggcagggat
    39301 tcgatatcac cgacagtacc gccgatttcg accagtacta cgtcatgacc ttcgccgcct
    39361 tccagcacgc gctctttgat agcgttagtg atgtgcggaa taacctgtac ggttgcgccg
    39421 agatagtcac cgcggcgttc tttacgcaga acgtcagagt agatacgacc cgtggtgaag
    39481 ttgttgcggc ggctcatttt ggtacgaatg aaacgctcgt agtgccccag gtccaggtcg
    39541 gtttcagcgc cgtcttcagt aacgaacact tccccgtgtt ggattgggct catagtaccg
    39601 ggatcgacgt tgatgtacgg atccagtttc atgatggtca cattgaggcc acgggcttca
    39661 agaatggctg cgagggaggc tgcggcaatg cctttaccca gagaggatac gaccccgccg
    39721 gtcacaaaaa tatagttcgt tgtcatgctg aacctgagaa gttaggttga aaagacgatg
    39781 gaataaccag gacgggaaag cagtataccc gaacatgacc tgtgccacaa actttcatta
    39841 tccctcctct tcgccagcgc actattgaaa tcaggagtga gaaaatagcc cctttggggt
    39901 aaatgttttt gacgcaaatc aagcgcttgt catttaaaaa atgacacaaa tcgcgcttga
    39961 ccacgtaatt cccttagaga tcaatttcct gccgttttac ctgttgccag acttcttcca
    40021 ttgtttcgag gtcaacacct gtcatttcca gtccacgcgc ggcaacaata cgctccactt
    40081 cgcgaaaacg acgctcgaat ttttcgttcg ctttttgcaa tgcgatttcc gcttttgtcc
    40141 ctaaatgacg agccagattg accgtggcaa acagcaggtc acccatttcc tcctccagtt
    40201 tagcctggtc gacaacagcc tgccgcgcct cgtacatcac ctcgtcgatc tcttcgtaga
    40261 ctttatcgac taccggacca agcgtcgtcc agtcgaaacc aacgttggcg caacgtttct
    40321 ggattttttg cgcacgcatt aaagccggta aactacgagg aatatcgtcc agcgccgaat
    40381 gctgcgcttt ctgcgcgcgc tcttcggttt tgatttgctc ccaacgggca agcacttcac
    40441 tactattttc ggcagaactg tcagcaaaaa catgcggatg gcgacgctct aatttatcgc
    40501 taatagcagc gcaaatatca ttaaagtcaa agcgcccttc ttcctgagct atttgtgcgt
    40561 aaaacaccac ctggaacagc agatcgccca gttcaccgcg cagatcgtca aaatcttcac
    40621 gggcgatggc gtccagcacc tcgtaggttt cttcaagggt gtaaggcgca atggtggcaa
    40681 atgtctgctc tttatcccac gggcagccgt tttccgggtc gcgcaggcgc tgcatgatgg
    40741 tgagcaaacg gtcgatttga ttcattgaat tatcctgaaa attgcgggtc cgtcaggtgt
    40801 aataccttcg agtaaaagat atcgctggat acggctgacg ccatatccag ctatgtaaga
    40861 ttaactcccg tgcaaccgac gcgcgtcgat aacatccggt acctggttga gtttacccag
    40921 cacgcgaccc agcacttgca ggttgtaaat ctcaatggtc atgtcgatgg tcgccagttg
    40981 ctgtttggtg tcgctacggc tggcaacgcc aagtacgttc accttctcgt tggcgagaat
    41041 ggtggtgata tcacgtaaca acccactacg atcattagcc accacgcgga ccaccagcga
    41101 atatccggcg gagtagctct cgccccatac cgcgtcaaca atgcgttctg gcgcatggga
    41161 gcgcagttcc gccaattgtt cgcaatcggc gcggtgtact gaaataccgc gcccctgggt
    41221 aatgaagccg acaatctcat ctccaggaat cggctggcag cagcgcgcga tgtggtgcat
    41281 caggttaccg accccttcta ccaccacacg accgttatct ttactgcggt tttgcggcgt
    41341 gtagcttttt tgctgaagct gtttcagcgc ggcggcgtcc tgctcttcgg cactcggctt
    41401 attaaattgc gattgcagga agttcaccat ctgattgaga cggatatccc cgccaccaat
    41461 cgccgccagc aactcgtcga catcattgaa gttgtaacgc ggcagcagat gtttttctgc
    41521 ttctttcagg ctgatcccca gatgttccag ctcgtcgtca aggatttgcc gcccagccag
    41581 aatgtttttg tcacggtcct gtttacggaa ccaggcgtga attttcgaac gcccacggct
    41641 ggttgtgacg taaccgaggt ttgggtttaa ccagtcacgg ctggggttcg gctgtttctg
    41701 ggtgataatt tcaatctggt cgcccatctg cagctggtag gtgaacggca caatgcgccc
    41761 gccaattttt gccccgatgc agcggtgtcc gacatcactg tggatgtggt aagcgaagtc
    41821 cagcggcgtt gatcccgcag gcaaatcaac gacatcacct ttcggcgtaa agacgtacac
    41881 tcggtcgtca aagacctgac tacgtacttc gtcgagcatt tcgccggaat cagccatctc
    41941 ttcctgccac gcaatcagtt tacgcagcca ggcaatccgg tcttcatgtc ccgaacgtgc
    42001 gccgccagca gccgcgccct ctttatattt ccagtgcgca gcaacaccca actctgcatc
    42061 ttcatgcatc tgtttggtgc ggatttggat ctcaacggtt ttgccacccg gccccagaac
    42121 cacggtatga atagactgat aaccgtttgg tttcgggtta gcgacgtaat cgtcaaactc
    42181 atccggcagg tggcgatagt gagtgtgcac tatccccagt gcggcataac aatcctgtaa
    42241 acgctcggcg acaatacgta ccgcacgcac atcaaacagc tcatcgaagg cgaggttctt
    42301 tttctgcatt ttgcgccaga tgctgtagat gtgtttcgga cggccataca cttcggcttt
    42361 aacgccttca gctttcatct cagcgcgcag atggccgaca aactcttcga tatagtgttc
    42421 gcggtcgaga cggcgttcat gcaacagttt tgcgatgcgt ttgtattcgg tcgggtggag
    42481 ataacggaag cagtaatctt ccagctccca tttcagttgc ccaatcccaa gacggttcgc
    42541 caacggcgcg tagatattgg tgcactcttt tgccgccagt acacgttcat cttccggcgc
    42601 atcttttact tcgcgcagat gagcaatacg ctccgccagt ttgatgacta cgcagcgaaa
    42661 atcatcgacc atcgccaata acatccggcg aacgttatcg acctgttcgg aggaaacaga
    42721 atcagtgtgc gtcgctttca gctggcggat cgccgccata tcacgcacgc cgtgaataag
    42781 gttaacgacc gacttaccga cgctctcgcg cagcacatct tcgctgacta cgttggcatc
    42841 agccagaggg aatagcagcg ccgcccgcag cgtgtcaatg tccatactta atgtcgagag
    42901 gatctccacc atctcaacac cacgccacaa taacagactg gcatccggat gcccctgcgt
    42961 ctgttgcaga caatacgccc aggtttcggc taagcactca cacgacttct ggctggtaat
    43021 acccagactt gcgatccatt tttccggatc aaattcacca gccttattga tatgtgcact
    43081 tcttaccgca accatcgtcc tctcctttag ggaccagacc tgccgaaatc ggcaaatcgt
    43141 aactatttaa cgcgcgagaa aagtaccatc gattccagat gtcccgtgtg tgggaacata
    43201 tccagcatcg ccagtcgcgc aatggtatat cctgctttta ataacgcttc gctatcccga
    43261 gccagcgttg cagggttaca ggatacataa actatacgaa taggttccag ttttataatt
    43321 tgctgcataa cacctgcggc acctgctcgc gccgggtcca acaacacttt atcgaagccg
    43381 tttttcgccc acggctgctt tgtgacatct tcttcaagat tttcgtgata aaacgtcaca
    43441 ttctgtaagc cattaagacg cgcattctgc tggccttttt ccaccagcgc cggaacacct
    43501 tctacaccga ccacactggc agcttgtgtc gccaatggca gtgtaaagtt gcccataccg
    43561 cagaacagat ccagtacgcg atcttcaggt tccacatcca gccattccaa cgcacgcgct
    43621 accatttttt ggttcacacc cgcattgacc tgaataaaat cgcgcgggct aaaagttaag
    43681 cgcaacccat ttgagtcata ccagggcatc tcaccagaga cggtttcgag tatctcacta
    43741 tcgggggcga gatacagatc caggccttca gaatgcgaaa agcattccag tttttcgcga
    43801 tctgccgaac ttagcggtgc ggtatggcgc aaaatcatta gcgtgccgct ggttgcctgt
    43861 accagttcaa catgaccaag atggcgcata gcttgtaagc tgcccaggca tgccctgact
    43921 ttgggcagca atgcttctag ttggggcact aaaatggggc attgtttaac gtcgacaatg
    43981 tcactggagc ccgctttgcg aaaccccatc tgaagttgct gtgttttcgg taagtagttc
    44041 agacttaaac gcgcgcgacg gcgatagccc caaggaacat cggcgatcac ttcagagact
    44101 tcgtgtttca ttaatcgggc gagtgccgca cttttgcttc gctgctgtaa atccacgctg
    44161 gcgtgttgtt gctgacagcc gccgcatacg ccaaaatgag gacagcgtgg cgtttcgcgt
    44221 tccgggctat cgcttaaccg gcgtacgact ttagcgcggg catactgttt tttatcttca
    44281 gtaacagtaa cttccgcgtt ttcctgcggc aataatccgg ggataaataa tgttttgccg
    44341 ttatgtcgcg ccacaccctg accaaaagag tcgaggtcgt tgactgaaac ggttatgatc
    44401 tgacgcgtcg tcgtgcgtcg ttttgcagag tagaattgcg ccattggcga gactttctca
    44461 atttaacagt gtgaccttaa ttgtcccata acggaactcc atgaccaact acagcctgcg
    44521 cgcacgcatg atgattctga tcctggcacc gaccgtcctt attggtttat tgctgagtat
    44581 ctttttcgtc gtgcatcgct ataacgactt gcagcgtcaa ctggaagatg ccggtgccag
    44641 cattattgag ccgcttgcag tttctactga atatggcatg agtctgcaaa atcgcgaatc
    44701 tatcggtcag ttaataagcg tactgcatcg tcgccattct gatattgttc gcgcgatttc
    44761 ggtttatgat gaaaataacc gactctttgt cacctccaat tttcatcttg atccctcatc
    44821 aatgcagctc ggcagcaacg tgccgtttcc tcgccagctc actgtcactc gtgacggcga
    44881 tattatgatc ctccgcacgc cgattatttc tgagagttac tcccccgacg aatcgcccag
    44941 tagcgatgcc aaaaatagtc aaaatatgtt gggatatatt gcgcttgagc tggatcttaa
    45001 atcggttcgc ttgcagcaat ataaagagat ctttatttcc agcgtgatga tgctgttttg
    45061 tatcggtatt gcgcttattt ttggctggcg cttaatgcgc gatgtaaccg gtccgattcg
    45121 caacatggtg aataccgtcg accgcatccg tcgcgggcaa ctcgacagcc gggtggaagg
    45181 atttatgctc ggcgagctgg atatgctgaa aaacggtatc aactcgatgg caatgtcgct
    45241 ggcggcttat cacgaagaga tgcaacacaa tatcgaccag gcgacgtccg atctgcgtga
    45301 gacgctggag caaatggaga ttcagaacgt tgagttggat ctggcgaaaa agcgcgccca
    45361 ggaagcggcg cgtattaaat ccgagtttct ggcaaatatg tcacacgagc tgcgtacacc
    45421 actgaatggt gttattggct ttacccgcct gacgctgaaa acagaattaa caccaacgca
    45481 gcgcgatcac ctgaatacga ttgaacgttc ggcaaataat ttgctggcaa ttattaatga
    45541 tgttctcgac ttctcgaaac tggaagccgg taagctgatt ctggaaagta ttccgttccc
    45601 actgcgcagc acgctggatg aagtcgttac ccttctggca cactcttccc acgataaagg
    45661 gctggaattg acgctcaata ttaagagcga cgtccccgat aacgtgattg gcgacccgct
    45721 acgtttacag caaatcatca ctaacctggt ggggaacgcg attaaattca ccgagaatgg
    45781 caacatcgat attctggtgg aaaaacgtgc gctgagtaat accaaagtgc aaattgaagt
    45841 gcagattcgg gataccggca ttggtattcc tgaacgcgat caatcgcgct tattccaggc
    45901 cttcagacag gctgatgcca gtatttcccg ccgtcatggt ggcaccgggc tggggctggt
    45961 gattacacaa aaactggtta atgaaatggg cggtgatatt tcgttccata gcctgccgaa
    46021 tcgtggttca actttctggt tccacattaa tctcgatctg aacccgaaca ttattatcga
    46081 agggccatcc acccagtgcc tcgctggtaa gcgcctggcc tatgtcgaac caaactccgc
    46141 agcagcacaa tgcacgctgg atattttaag tgaaacgccg ctggaagtgg tttatagccc
    46201 aacgttctcc gcgctgcctc ccgcgcatta cgacatgatg ttgttaggca tcgcggtgac
    46261 cttccgcgag ccgctaacaa tgcaacatga gcgattagcg aaagcggtat cgatgaccga
    46321 tttcctgatg ctggcacttc cttgccatgc acaagtcaat gctgaaaaac ttaagcaaga
    46381 tggtatcggc gcgtgtctgc tgaaaccatt aacacctacg cgcctgttgc ctgccctgac
    46441 ggaattttat catcacaaac aaaacacgct tttgcctgta accgatgaaa gtaagctggc
    46501 aatgacagtc atggcggttg atgacaaccc cgctaacctg aaacttatcg gcgcattgct
    46561 ggaagatatg gtgcaacatg tggaactttg cgatagcggg catcaggcag ttgaacgggc
    46621 gaaacagatg ccgttcgatt taatcttaat ggatattcaa atgccggaca tggacggcat
    46681 tcgggcatgt gagctcatcc accagctccc gcatcagcag caaacgccgg ttatcgcggt
    46741 aacggcgcat gcgatggccg ggcaaaaaga gaaactgctt ggcgcgggga tgagtgatta
    46801 tctcgccaaa ccgatcgaag aagagcgatt gcataatttg ctgttgcgct acaagcctgg
    46861 cagcggtatt tcctctcgcg tcgtgacgcc cgaagtcaac gaaattgtgg tcaacccgaa
    46921 tgcgaccctc gactggcaac tggcactacg ccaggcagca ggaaaaaccg atttagcgcg
    46981 cgatatgctg caaatgttac tcgatttcct gcctgaagtt cgcaacaaag ttgaggaaca
    47041 gctggttgga gaaaacccgg aaggcctggt tgatttgatt cataaactgc atggcagttg
    47101 cggctatagc ggtgtaccgc gtatgaagaa tctctgccaa ctgatcgaac aacagctacg
    47161 tagcggtact aaagaagaag atttggagcc ggagctgctg gaactgttgg acgagatgga
    47221 taatgtagcg cgcgaagcca gcaaaattct cgggtaatgg acgtcttgtc gagtagcaac
    47281 cggattttta tgacgctggt ttcagattgg tgacaagtgc ctgtgtttat gccggatgcg
    47341 gcgttaacgc cttatccggc ctgcaaaacc aattaaattc aatgaattaa aaaatgatgt
    47401 agccctgata cgtagcgcaa caggcaattt tgcgtttggc atcagtctca aaccggctcc
    47461 agatagagcc ggttttggtt ttctgtctta acgcaccatg cacggacgct tgttatcgaa
    47521 cgtccagccg gggatcaggt actgcattcc catcgcatcg tcacgcgcgc caagcccgtg
    47581 tttctgatac agctcgtgcg ctttcatcac ttgatccata tcgatttcta cacccagccc
    47641 cggtttttct ggcacctgaa ccagaccgcc tttgatctct aacggttctt tggtcaggcg
    47701 ctgattacct tcctgccaga tccagtgcgt atcaatcgcg gtgattttac ccggtgcggc
    47761 agcggcaaca tgggtaaaca tcgccaggga aatatcgaag tggttgttgg agtgtgaacc
    47821 ccaggtcagg ccaaattcat gacacatttg cgccacacgt accgaacctt gcatcgtcca
    47881 gaaatgcgga tccgcaagcg ggatatcaac ggattgcaac gagagcgtat ggcccatctg
    47941 ccgccagtcg gtggcgatca tattggttgc ggtcggcagg ccagttgcgc gacggaactc
    48001 tgccatcact tcacgcccgg agaaaccttg ctccgcacca cacggatctt ctgcataagc
    48061 cagcgaacct ttcaggtatg taccgatttt aatcgcttcg ttcagcgacc aggcaccgtt
    48121 aggatcgagc gtaatacgcg cctgcgggaa acgttgcgcc agtgccacaa tagattctgc
    48181 ttcttcttcc ccggccagta caccgccctt cagtttgaaa tcgttgaagc catatttttc
    48241 atatgccgct tccgccaggc gcaccaccgc atccggcgtc atcgcttctt cgtgacgcag
    48301 gcgataccag tcacattgat catccggctg gctttgatac ggcagcggtg tggctttgcg
    48361 attaccgacg aagaacagat aaccgagcat ttcgacttcg ctacgctgtt ggccatcgcc
    48421 cagcagcgat gccacgttta cccccagatg ctgccccaac agatccagca ttgccgcttc
    48481 tatcccggta actacatgaa tagtggtacg caaatcaaat gtctgcaaac cgcgcccacc
    48541 ggcatcacga tcggcaaacg tattacgcac cagcgtcaga acgtttttgt attcacccag
    48601 cgttttaccc accaccagcg gaatcgcatc ttccagcgtt ttacggattt tctcgccgcc
    48661 gggaatttcc cctacgccag tgtgaccgga attatctttg ataatcacaa tattacgcgt
    48721 gaagaacggt gcatgtgcac cactcagatt catcagcata ctgtcatgac ccgccaccgg
    48781 gatgacctgc atttcagtaa caacaggcgt cgtaaattga gaactcatat ttatgtcctt
    48841 tttcagaatc aatgacggcc gaaaacgggg cgtttacggt caaaggtcca gccggggatc
    48901 aggtactgca tcgggcctgc gtcgttacgc gcaccgccag gcagacgttt ataggcctca
    48961 tgtgcctttt gtacctgttc ccagtccagt tccacgccca gaccgggcgc atcaggaacg
    49021 gcaatttttc cgtttttaat ctccagcgga tttttggtca gacgacaatc gccctcctgc
    49081 caaatccagt gggtatcgat agcggtagga ttacccggtg ccgccgcgcc cacatgggta
    49141 aacatcgcca gagagatatc aaagtggtta ttagaatggc agccccaggt cagcccccag
    49201 tcgtcgcaaa gttgcgccac gcggactgca ccggaaagcg tccagaagtg cggatcggca
    49261 agtggaatat ctaccgcatt gagcatcacc gcatgaccca tttcgcgcca gttggtggca
    49321 atcatgttgg tcgcgacggg caagccggtc gcccgtcgaa actccgccat cacttcacga
    49381 ccggagaaac cctgctcagc gccgcacggg tcttccgcat aggtaagaac atcattcagc
    49441 cctttgcaca gagaaatggc ttcatcaagc agccaggcac cattgggatc aacggtaatc
    49501 cgcgcatccg ggaagcgttt cttcaatgca cgaacagtgt cgatttcttg ctcgccaggt
    49561 aacacgccac ccttaagttt gaaatcttta aagccatagc gatcctgtga ggcttccgcc
    49621 agacgcacaa cggcttcgct gttcatcgct ttctgatgcc gcaactgata ccactcatgg
    49681 ttgcccggcg tattttccag ataaggaaga tcggttttgg tccgatcacc gatataaaac
    49741 agataaccga gaacggtaat agcatcacgt tgcttgcctg gccctaacag ttcgcagacc
    49801 ggaacattca gcgcctgacc taacaggtca agcaaggcgg cttccagcgc cgctacggca
    49861 ttaacgcgca attcaaaagt ccaggcacct ttgccgaagg tatcaaaatc ggctgcctga
    49921 ttacctttat gcacctgctg aaccacttta ttcaggcgcg caacttcctg gcccagcacc
    49981 atcggaatag ccccgaccag tgtttgataa atcacctctc cgcccggcgc ttcaccaatg
    50041 ccggtatgcc cggcattatc ggtgagtacc acaatattgc gggtgaaata tgcgttatgt
    50101 gcaccaccaa tattaagcaa catgctgtca tgtccggcga ccggaatgac tttcatatca
    50161 gtaataacag gactggattg tgtcgtcatc ttattgcccc gcgacaggtt tcagctcaat
    50221 acgcttgata tcgcccacca gcaccaggta gctgagtacc gcgattaagg catgaacacc
    50281 aacataaatc agcgccccat taaaggagcc agtcgtgcca acgatataac caattgcgat
    50341 tggcgtgacg atgccagaaa tgttaccaaa catgttgaac aggccaccgg aaagaccgct
    50401 gatctctttt ggcgcagtat cagccattac agcccaaccc agcgcaccga tccccttacc
    50461 gaagaaggcc agcgccataa agccgatgat catccactca acgttgacgt agttgcagaa
    50521 caccatcacc atcgacaaca acatgcccat cacgatcggt gttttacgcg caatgttcag
    50581 tgaacccgtg cgacgcatca gccagtcgga aataatcccc cccaacacac cgccgataaa
    50641 accgcaaacc gccggaacgg aagccacaaa gcccgctttc agaatcgaca tcccgcgcgc
    50701 ctgcaccaga taaaccggga accaggtaat gaaaaagtaa gtcagggcgt tgatacagta
    50761 ctgaccgata taaacgccga tcatcatccg tgacccgagc agctgtttga tctgccccca
    50821 cttcacgctg aacggcactt taactttggt gttttgctga tccatattga tcagcgcgcc
    50881 acccgcggcg atgtactcca gctctttctg atttacgccc ggatgttgat ttggctcgtg
    50941 gatgactttc aaccagataa agctgatgac aatgcccaga ccgcccataa agaagaagac
    51001 gtgtgaccag cccacttcat gagtcagcca gcccataatc ggcgcgaaga taaccgttgc
    51061 gaagtattgg gcggagttaa aaatcgacac cgccgttccc ctttcctgcg ccggaaacca
    51121 ggccgcaaca atgcggctgt tgccagggaa agatggcgct tcagcaagcc cgaccaggaa
    51181 gcgcagtgta aacagggcca cgataatgcc gaatccacta aagatatcga cgaagccttg
    51241 cagcaaggta aacatcgacc agataaagat cgaccagaag tagacgcgtt ttgaaccaaa
    51301 acgatccagc aaccagccac cagggatctg cccgataaca taagcccatg agaaggcaga
    51361 gaacacatag cccattccca cgggatcaag gccgatatct ttggccattt ccgaaccggc
    51421 aatagaaagc gtagcgcggt cgccgtagtt gaaggatgtg acgataaaca acatcaccac
    51481 tatccagtaa cgagcattcg tgcgtttttc cacactgctc gcagcctgac ttaaagaact
    51541 cattgttgca ctcctgaaaa ttcgcgttag ccacgctcac tctggactgc gacatcgcca
    51601 ggaaatcaga ggtgacgtag ggtgtttttg ccgtttttat aggtcgttcg ccgaatacgg
    51661 cgcgtgttta tatctggcaa tagcagtata aaaagcgcgc catagcggct caccgtgcaa
    51721 caacacaaca ttaatgcgtt caatgaggcc cgattttggc attagccctg gacggtggaa
    51781 tccacttcac ggaaatgaaa acaagaacaa gaaaggaagg gttaaaacga agaaataaag
    51841 aagagtatga aatggatcgc ttgactccag gcaaacgcca gtaaaaatcc gcgctatgaa
    51901 gcagttttta ctggcatttg cctgaaaaga ttcgattcag caccgctaaa acggcattta
    51961 ccgctcgctg aacatatcag gacaacatcg tgccccaatg ttcaacccac ggatttgatt
    52021 ccgtttccgg ttccgggttc tcactggcgt caatcaacag catttcgcca acccgctgag
    52081 cgctctgttc ctgcaacaag gcatcgaact gtttgccgcc attgcagaaa ttcacataac
    52141 tactgtcgcc gagcgcaatc acgccataac gcagattcgg ctggaagccc agggtatctt
    52201 taattccctg aaagagcggc acaatgctat caggaaggtc gccctgcccg gtcgtggacg
    52261 taaccaccag aacatactta tcctgatagg gcagccagtc gcttaattca ggatcttcaa
    52321 ataccgttgc tttgtggccc tgcgcggtca gaatcgcttc cgcttcttcg gccactaaca
    52381 gcgaatttcc gtacatagtg ccgacaaaaa taccaatttc cgccataccg ttttctccct
    52441 ggattaggaa cttatctctc catcctgacc cgatggcgca ctgaactcaa ccctttcatt
    52501 ttcaggaagc agaccccgcc agccaaattg tgataacgcc tgcatccagg tgtcgtccag
    52561 gcccgcgtga atagccagcg gctcgccagt aaaaggatgc gtcaatgaca actgactggc
    52621 atgcagcatt aaccgctgaa ggccaaaatg ctcagcaccg ctgcgattct ggcgtaaatc
    52681 accatgtttg ctgtcgccaa taatcgggtg ccgcaaatgg gcaagatggc gacgaagctg
    52741 gtgcttgcgt ccagttttcg gctccagttc caccaggccg tagcgcgtgg tcgggtaacg
    52801 tccagtcgct accggcattt ctacggtcgc cagaccgcga taatgcgtca ctgctggctg
    52861 cgggccttta tcttcgcggg caaatttatc agcgattttg tccagttctt ccaccagtgg
    52921 ataatccagc accgcttctt ccatcaacca gccacgcaca atcgcatggt aacgtttctg
    52981 gatttggtgc tgttcaaact gttgtgccag cagccgtccg gcttcgctgg ataatcccat
    53041 caacaacaca ccagaagttg gtcggtccag acgatgagca gtaaaaacat gctggcctat
    53101 ctggtcacgc acggtttgca tgaccactac tttctcgtcg cgatccagcc agctgcggtg
    53161 aaccagccag ccggagggtt tatttaccgc aaccagccat tcatcctgat agagtatttc
    53221 cagcattagc tcgcatcatc cgcaaaaaga gcatccagtt tctccagctc agccagaata
    53281 agcgcgcgtt gcggatgatc agtcgccagc gccatttcat aatagggcgc aacagcaaaa
    53341 gcgcccggta acggctggtt gttatctaac aaatcgtgca ttcgcgggat cagcacccac
    53401 tgcaaccact ccagtggttc catggtgtcc ataaagaacg gttgggtact attaaattga
    53461 tgcggctggg gttcatcgtt tcgccagtgc tgatgttcac gcagtaacgc ttcaagcgcc
    53521 tgcaactgga gacgaacgcg gtcatgagtg gtcataaagc aacctcaata agaaaaactg
    53581 ccgcgaagga tagcaggaat aaaaaaaggg agcactgtat tcacagtgct cccggttcgt
    53641 ttcgcagcat tccagctact tttgttgctc cctgctcatc cttgacaact tttcctctgg
    53701 ccttgcggcc aatcgttcat cctgaactat tgcttcctgc tcacaccacc ccgatgtgat
    53761 acttcatcct gaagtgtccc tggccttcct gacccaccga atcatcctga ccggttctca
    53821 ttctccatcc tggaggtgtc ctttaacgcg tcctgcgtca tcctcttcgc ttcatccaga
    53881 agcctttccc tgaaacacca tcctggtgtg tccttcagaa gtgtcatcat cctgatgttc
    53941 acttcgttgt ctgactccct gtcgacgaag atagaatcgt ctttttctcg aagtcttaca
    54001 aggcgcttac agacaatgcc ttaaggaaaa tttcatatga aagttaagaa ttaacaagca
    54061 acatgttgtt tttaaatgag aataacgatt cttatctttt taagtattcg cttatttcct
    54121 ggcgatctcc tacagagcct gtaagagatc tctcacaacc agatggggta tttggattac
    54181 agaagaggct taagttgatt aaggaattcc gcaagatttg aggctaaatg cgtccgtttg
    54241 cgtgttccca gcgtctcttt gcacacttca ccggagagat tacacaccga gatgacctct
    54301 aactcttctt ccagcgtcgc gataaatagc gtgggcggca gttttagcct tttctgagtc
    54361 accagatgac caatcagatt ttcctgcacc cgacggaagt cgtcttcact ccaggtttgc
    54421 agcaatgtca gtttgatatc gccaaactgc gcgtgcatat ccccggcaaa ctgagtcgta
    54481 taaaaggtat gaatcgtggg ttgtatcaca agatcaaaag cgcgttcaac cgcgtttaca
    54541 ttttgttccc cggtaaacgg ctggggttgc cagtatacgg cgtcttcggt ggttgaaata
    54601 atgcacggtg aaggaacgcc gtacagttcc tcactcaacg gccagctttt atgctcttca
    54661 tgccacgcat cgcagtagcg tgcagtaaaa tctttcaggg cttgtgcggt caaatcgtcc
    54721 actaatttct ctcttcactt aaaccagata cacttgtcgt ttagtttatc tggtttatga
    54781 cggtgaaaca tgtcttctta tgcaaaccac caggcacttg cgggcctgac tcttggaaaa
    54841 tcaaccgatt accgggatac ctatgacgcc agcctactgc aaggcgttcc gcgtagtctg
    54901 aatcgcgatc cgctgggtct gaaagcggat aacctgcctt ttcacggtac ggatatctgg
    54961 acgctatatg aactttcctg gctgaatgcg aaaggtttgc cgcaggtcgc cgtcggtcat
    55021 gttgaacttg attacaccag cgcaaatctg attgagtcga agagttttaa gctctatctc
    55081 aacagtttta accagacgcg ttttaataac tgggatgagg tgcgccagac gctggagcgc
    55141 gacttaagca cttgcgccca gggtaaggtt agcgtggcgt tatatcgtct tgatgaactg
    55201 gaaggccaac cgataggtca tttcaatggc acttgcattg atgaccagga tatcactatc
    55261 gataactatg aattcactac tgactatctg gagaatgcca ccagtggcga aaaagtagta
    55321 gaagagacgc tcgtcagcca cctgctgaaa tcaaactgcc tgatcaccca tcaaccagat
    55381 tggggatcga tccaaattca gtatcgtgga cgccaaattg acagagaaaa actgctgcgt
    55441 tacctggttt cttttcgtca tcacaacgag ttccacgaac agtgcgtgga acgcatcttt
    55501 aatgacctgt tacgcttctg ccagccagag aaattgagcg tttacgcacg ttatacccgt
    55561 cgtggcggtc tggacattaa cccgtggcgc agtaatagcg attttgtccc atcgaccaca
    55621 agactggttc ggcaataaat tttttctcaa ttttgcgtgc tggattcacg cagaaggttg
    55681 tgaaaggtca tcaggcaggg ctattgtaat caaagggaat gacgatattc gtcccataag
    55741 gagtttttct tgattacaca tattagcccg cttggctcca tggatatgtt gtcgcagctg
    55801 gaagtggata tgcttaaacg caccgccagc agcgacctct atcaactgtt tcgcaactgt
    55861 tcacttgccg tactgaactc cggtagtttg accgataaca gcaaagaatt gctgtctcgt
    55921 tttgaaaatt tcgatattaa cgtcctgcga cgtgaacgcg gcgtaaagct ggaactgata
    55981 aatcccccgg aagaggcttt tgtcgatggg cgaattattc gcgctttgca ggccaacttg
    56041 ttcgcggttc tgcgtgacat tctcttcgtt tatgggcaaa ttcataacac cgttcgtttt
    56101 cccaacctga atctcgacaa ctccgtccac atcactaacc tggtcttttc catcttgcgt
    56161 aacgctcgcg cgctgcatgt cggtgaagcg ccaaatatgg tggtctgctg gggcggtcac
    56221 tcaattaacg aaaacgagta tttgtatgcc cgtcgcgtcg ggaatcagtt gggcctgcgt
    56281 gaactgaata tctgcaccgg ctgtggtccg ggagcgatgg aagcgccgat gaaaggtgct
    56341 gcggtcggac acgcgcagca gcgttacaaa gacagtcgct ttattggtat gacagagccg
    56401 tcgattatcg ccgctgaacc gcctaacccg ctggtcaacg aattgatcat catgccagat
    56461 atcgaaaaac gtctggaagc gtttgtccgt atcgctcacg gtatcattat cttccctggc
    56521 ggtgtgggta cagcagaaga gttgctgtat ttgctgggaa ttttaatgaa cccggccaac
    56581 aaagatcaag ttttaccatt gatcctcacc ggcccgaaag agagcgccga ctacttccgc
    56641 gtactggacg agtttgtcgt gcatacgctg ggtgaaaacg cgcgccgcca ttaccgcatc
    56701 atcattgatg acgccgctga agtcgctcgt cagatgaaaa aatcgatgcc gctggtgaaa
    56761 gaaaatcgcc gcgacactgg tgacgcttac agctttaact ggtcaatgcg cattgcgcca
    56821 gatttgcaaa tgccgtttga gccgtctcac gagaatatgg ctaatctgaa gctttacccg
    56881 gatcaacccg ttgaagtgct ggctgccgac ctgcgccgtg cgttctccgg tattgtggcg
    56941 ggtaatgtaa aagaagtcgg tattcgcgcc attgaagagt ttggtcctta caaaatcaac
    57001 ggcgacaaag agattatgcg tcgtatggac gacctgctac agggttttgt tgcccagcat
    57061 cgtatgaagt tgccaggctc agcctacatc ccttgctacg aaatctgcac gtaatctccc
    57121 ctcccccggt gacgtttgtt gccgggtttt atcgtttttg gtcacttact catcaactca
    57181 tttcatttgt tatatgaatg tttcttacca acctcacgcg aca
//
LOCUS       DAHHTG010000012        55319 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.012, whole genome shotgun sequence.
ACCESSION   DAHHTG010000012 DAHHTG010000000
VERSION     DAHHTG010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 55319)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 55319)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..55319
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.012"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            207..830
                     /gene="nfeR"
                     /locus_tag="NBI74_000747"
                     /gene_synonym="yqjI"
     CDS             207..830
                     /gene="nfeR"
                     /locus_tag="NBI74_000747"
                     /gene_synonym="yqjI"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NfeR"
                     /protein_id="HCD1931122.1"
                     /translation="MSHHHEGCCKHEGQPRHEGCCKGEKSEHEHCGHGHQHEHGQCCG
                     GRHGRGGGRRQRFFGHGELRLVILDILSRDDSHGYELIKAIENLTQGNYTPSPGVIYP
                     TLDFLQEQSLITIREEEGGKKQIALTEQGAQWLEENREQVEMIEERIKARCVGAALRQ
                     NPQMKRALDNFKAVLDLCVNQSDITDAQIKKIIAVIDRAAFDITQLD"
     gene            complement(937..2457)
                     /gene="aer"
                     /locus_tag="NBI74_000748"
     CDS             complement(937..2457)
                     /gene="aer"
                     /locus_tag="NBI74_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerotaxis sensor receptor Aer"
                     /protein_id="HCD1931123.1"
                     /translation="MSSHPYVTQQNTPLADDTTLMSTTDLQSYITHANDTFVQVSGYT
                     LQELQGQPHNMVRHPDMPKAAFADMWFTLKKGEPWSGIVKNRRKNGDHYWVRANAVPM
                     VREGKISGYMSIRTRATDEEIAAVEPLYKALNAGRTGKRIHKGLVVRKGWLGKLPSLP
                     LRWRARGVMTLMFIVLAAMLWFVAAPVVTYIFCALVMLLGNACFEWQIVRPIENVASQ
                     ALKVATGERNSVEHLKRSDELGLTLRAVGQLGLMCRWLINDVSSQVSSVRNGSETLAK
                     GTDELNEHTQQTVDNVQQTVATMNQMAASVKQNSATASAADKLSITASNAAVQGGEAM
                     TTVIKTMDDIADSTQRIGTITSLINDIAFQTNILALNAAVEAARAGEQGKGFAVVAGE
                     VRHLASRSANAANDIRKLIDASADKVQSGSQQVHAAGRTMEDIVAQVKNVTQLIAQIS
                     HSTLEQADGLSSLTRAVDELNLITQKNAELVEESAQASAMVKHRASRLEDAVTVLH"
     gene            2875..4254
                     /gene="ygjG"
                     /locus_tag="NBI74_000749"
     CDS             2875..4254
                     /gene="ygjG"
                     /locus_tag="NBI74_000749"
                     /EC_number="2.6.1.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417544.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine aminotransferase"
                     /protein_id="HCD1931124.1"
                     /translation="MNRLPSSASALACSAHALNLIEKRTLDHEEMKALNREVIEYFKE
                     HVNPGFLEYRKSVTAGGDYGAVEWQAGGLNTLVDTQGQEFIDCLGGFGIFNVGHRNPV
                     VVSAVQNQLAKQPLHSQELLDPLRAMLAKTLAALTPGKLKYSFFCNSGTESVEAALKL
                     AKAYQSPRGKFTFIATSGAFHGKSLGALSATAKSTFRKPFMPLLPGFRHVPFGNIEAM
                     RTALNECKKTGDDVAAVILEPIQGEGGVILPPPGYLTAVRKLCDEFGALMILDEVQTG
                     MGRTGKMFACEHENVQPDILCLAKALGGGVMPIGATIATEEVFSVLFDNPFLHTTTFD
                     GNPLACAAALATINVLLEQNLPAQAEQKGDMLLDGFRQLAREYPDLVQEARGKGMLMA
                     IEFVDNEIGYNFASEMFRQRVLVAGTLNNAKTIRIEPPLTLTIEQCELVIKAARKALA
                     AMRVSVEEA"
     gene            complement(4296..4628)
                     /locus_tag="NBI74_000750"
     CDS             complement(4296..4628)
                     /locus_tag="NBI74_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
                     /protein_id="HCD1931125.1"
                     /translation="METVAYADFARLEMRVGKIVEVKRHENADKLYIVQVDVGEKTLQ
                     TVTSLVPYYSEEELMGKTVVVLCNLQKAKMRGETSECMLLCAETDDGSESVLLTPERM
                     MPAGVRIV"
     gene            4847..5830
                     /gene="ebgR"
                     /locus_tag="NBI74_000751"
     CDS             4847..5830
                     /gene="ebgR"
                     /locus_tag="NBI74_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator EbgR"
                     /protein_id="HCD1931126.1"
                     /translation="MATLKDIAIEAGVSLATVSRVLNDDPTLNVKEETKHRILEIAEK
                     LEYKTSSARKLQTGAVNQHHILAIYSYQQELEINDPYYLAIRHGIETQCEKLAIELTN
                     CYEHNGLPDIKNVTGILIVGKPTSALRAAACALTDNICFIDFHEPGSGYDAVDIDLAR
                     ISKEIIDFYINQGVNRIGFIGGEDLPGKADIREVAFAEYGRLKHVVREEDIWRGGFSS
                     SSGYELAKQMLAREDYPKALFVASDSIAIGVLRAIHERGLNIPQDISLISVNDIPTAR
                     FTFPPLSTVRIHSEMMGSQGVNLVYEKARDGRALPLLVFVPSKLKLRGTTR"
     gene            6014..9106
                     /gene="ebgA"
                     /locus_tag="NBI74_000752"
     CDS             6014..9106
                     /gene="ebgA"
                     /locus_tag="NBI74_000752"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit alpha"
                     /protein_id="HCD1931127.1"
                     /translation="MNRWENIQLTHENRLAPRAYFFSYDSVAQARTFARETSSLFLSL
                     SGQWNFHFFDHPLQVPEAFTSELMADWGHITVPAMWQMEGHGKLQYTDEGFPFPIDVP
                     FVPSDNPTGAYQRIFTLSDGWQGKQTLIKFDGVETYFEVYVNGQYVGFSKGSRLTAEF
                     DISAMVKTGDNLLCVRVMQWADSTYVEDQDMWWSAGIFRDVYLIGKQLTHINDFTVRT
                     DFDEAYCDATLSCEVVLENLAASPVVTTLEYTLFDGERVVHSSAIDHLAIEKLTSARF
                     DFTVEQPQQWSAESPYLYHLVMTLKDADGNVLEVVPQRVGFRDIKVRDGLFWINNRYV
                     MLHGVNRHDNDHRKGRAVGMDRVEKDLQLMKQHNINSVRTAHYPNDPRFYELCDIYGL
                     FVMAETDVESHGFANVGDISRITDDPQWENVYVERIVRHIHAQKNHPSIIIWSLGNES
                     GYGCNIRAMYHAAKALDDTRLVHYEEDRDAEVVDIISTMYTRVPLMNEFGEYPHPKPR
                     IICEYAHAMGNGPGGLTEYQNVFYKHDCIQGHYVWEWCDHGIQAQDDNGNVWYKFGGD
                     YGDYPNNYNFCLDGLIYSDQTPGPGLKEYKQVIAPVKIHALDLTRGELKVENKLWFTT
                     LDDYTLHAEVRAEGETLATQQIKLCDVAPNSEAPLQITLPQLDAREAFLNITVTKDSR
                     TRYSEAGHPIATYQFPLKENTAQPVPFAPNNARPLTLEDDRLSCTVRGYNFAITFSKM
                     SGKPTSWQVNGESLLTREPKINFFKPMIDNHKQEYEGLWQPNHLQIMQEHLRDFAVEQ
                     SDGEVLIISRTVIAPPVFDFGMRCTYIWRIAADGQVNVALSGERYGDYPHIIPCIGFT
                     MGINGEYDQVAYYGRGPGENYADSQQANIIDIWRSTVDAMFENYPFPQNNGNRQHVRW
                     TALTNRHGNGLLVVPQRPINFSAWHYTQENIHAAQHCNELQRSDDITLNLDHQLPGLG
                     SNSWGSEVLDSWRVWFRDFSYGFTLLPVSGGEATAQSQASYEFGAGFFSTNLHSENKQ
                     "
     gene            9103..9552
                     /locus_tag="NBI74_000753"
     CDS             9103..9552
                     /locus_tag="NBI74_000753"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit beta"
                     /protein_id="HCD1931128.1"
                     /translation="MRIIDNLEQFRQIYASGKKWQRCVEAIENIDNIQPGVAHSIGDS
                     LTYRVETDSATDALFTGHRRYFEVHYYLQGQQKIEYAPKETLQVVEYYRDETDREYLK
                     GCGETVEVHEGQIVICDIHEAYRFICNNAVKKVVLKVTIEDGYFHNK"
     gene            9615..11048
                     /locus_tag="NBI74_000754"
     CDS             9615..11048
                     /locus_tag="NBI74_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCD1931129.1"
                     /translation="MSDTKRNTIGKFGLLSLTFAAVYSFNNVINNNIELGLASAPMFF
                     LATIFYFIPFCLIIAEFVSLNKNSEAGVYAWVKSSLGGRWAFITAYTYWFVNLFFFTS
                     LLPRVIAYASYAFLGYEYIMTPVATTIISMVLFAFSTWVSTNGAKMLGPITSVTSTLM
                     LLLTLSYILLAGTALVGGVQPADPITVDAMIPNFNWAFLGVTTWIFMAAGGAESVAVY
                     VNDVKGGSKSFVKVIILAGIFIGVLYSVSSVLINVFVSSKELKFTGGSVQVFHGMAAY
                     FGLPEALMNRFVGLVSFTAMFGSLLMWTATPVKIFFSEIPEGIFGKKTVELNENGVPA
                     RAAWIQFLIVIPLMIIPMLGSNTVQDLMNTIINMTAAASMLPPLFIMLAYLNLRAKLD
                     HLPRDFRMGSRRTGIIVVSMLIAIFAVGFVASTFPTGANILTIIFYNVGGIVIFLGFA
                     WWKYSKYIKGLTAEERHIEATPASNVD"
     gene            11182..12252
                     /gene="ygjJ"
                     /locus_tag="NBI74_000755"
     CDS             11182..12252
                     /gene="ygjJ"
                     /locus_tag="NBI74_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgjJ"
                     /protein_id="HCD1931130.1"
                     /translation="MKLITAPCRALLALPFCYAFSALGEEARPAEHDDTKTPEMISTS
                     SPSFRFYGELGVGGYMDLEGENKHKYSDGTYIEGGLEMKYDSWFGLIYGEGWTVQADH
                     DGNAWVPDHSWGGFEGGINRFYGGYRTNDGTEIMLSLRQDSSLDDLQWWGDFTPDLGY
                     VIPNTRDIMTALKVQNLSGNFRYSVTATPAGHHDESKAWLHFGKYDRYDDKYTYPAMM
                     NGYIQYDLAEGITWMNGLEITDGTGQLYLTGLLTPNFAARAWHHTGRADGLDVPGSES
                     GMMVSAMYEALKGVYLSTAYTYAKHRPDHADDETTSFMQFGIWYEYGGGRFATAFDSR
                     FYMKNASHDPSDQLFLMQYFYW"
     gene            12269..14620
                     /gene="ygjK"
                     /locus_tag="NBI74_000756"
     CDS             12269..14620
                     /gene="ygjK"
                     /locus_tag="NBI74_000756"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HCD1931131.1"
                     /translation="MKIKTILTPVACALLMSFSAHAANADNYKNVINRTGAPQYMKDY
                     DYDDHQRFNPFFDLGAWHGHLLPDGPNTMGGFPGVALLTEEYINFMASNFDRLTVWQD
                     GKKVDFTLEAYSIPGALVQKLISKDVQVEMILRFATPRTSLLETKITSDKPLDLVWDG
                     ELLEKLEAKEGKPLSDKTIAGEYPDYQRKISATRDGLKVTFGKVRATWDLLTSGESEY
                     QVHKSLPMQTEINGNRFTSKAHINGSTTLYTTYSHLLTAQEVSKEQMQIRDILARPAF
                     YLTASQQRWEEYLKKGLTNPDATPEQTRVAVKAIETLNGNWRSPGGAVKYNTVTPSVT
                     GRWFSGNQTWPWDTWKQAFAMAHFNPDIAKENIRAVFSWQIQPGDRVRPQDVGFVPDL
                     IAWNLSPERGGDGGNWNERNTKPSLAAWSVMEVYNVTQDKAWLAEMYPKLVAYHDWWL
                     RNRDHNGNGVPEYGATRDKAHNTESGEMLFTVKKGDKEETQSGLNNYARVVEKGQYDS
                     LEIPAQVAASWESGRDDAAVFGFIDKEQLDKYVANGGKRSDWTVKFAENRSQDGTLLG
                     YSLLQESVDQASYMYSDNHYLAEMATILGKPEEAKRYRQLAQQLADYINTCMFDPTTQ
                     FYYDVRIEDKPLANGCAGKPIIERGKGPEGWSPLFNGAATQANADAVVKVMLDPKEFN
                     TFVPLGTAALTNPAFGADIYWRGRVWVDQFWFGLKGMERYGYRDDALKLADTFFRHAK
                     GLTADGPIQENYNPLTGSQQGAPNFSWSAAHLYMLYNDFFRKQ"
     gene            14846..16864
                     /gene="fadH"
                     /locus_tag="NBI74_000757"
     CDS             14846..16864
                     /gene="fadH"
                     /locus_tag="NBI74_000757"
                     /EC_number="1.3.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 2,4-dienoyl-CoA reductase"
                     /protein_id="HCD1931132.1"
                     /translation="MSYPSLFAPLDLGFTTLKNRVLMGSMHTGLEEYPDGAERLAAFY
                     AERARHGVALIVSGGIAPDLTGVGMEGGAMLNDANQIPHHRTITEAVHQEGGKIALQI
                     LHTGRYSYQPHLVAPSALQAPINRFVPHELTHEEILQLIDDFAHCAQLAREAGYDGVE
                     VMGSEGYLINEFLTLRTNQRSDQWGGDYRNRMRFAVEVVRAVRERVGNDFIIIYRLSM
                     LDLVEGGGTFAEMVELAQAIEAAGATIINTGIGWHEARIPTIATPVPRGAFSWVTRKL
                     KGHVSLPLVTTNRINDPQVADDILSRGDADMVSMARPFLADAELLSKAQSGRADEINT
                     CIGCNQACLDQIFVGKVTSCLVNPRACHETKMPILPAVQKKNLAVVGAGPAGLAFAIN
                     AAARGHQVTLFDAHSEIGGQFNIAKQIPGKEEFYETLRYYRRMIEVTGVTLKLNHTVT
                     ADQLQAFDETILASGIVPRVPPIDGIDHPKVLSYLDVLRDKAPVGNKVAIIGCGGIGF
                     DTAMYLSQPGESTSQNIAGFCNEWGIDSSLQQAGGLSPQGMQIPRSPRQIVMLQRKAS
                     KPGQGLGKTTGWIHRTTLLSRGVKMIPGVSYQKIDDDGLHVVINGETQVLAVDNVVIC
                     AGQEPNRALAQPLIDSGKTVHLIGGCDVAMELDARRAIAQGTRLALEI"
     gene            complement(16909..17325)
                     /gene="higA"
                     /locus_tag="NBI74_000758"
     CDS             complement(16909..17325)
                     /gene="higA"
                     /locus_tag="NBI74_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HigA"
                     /protein_id="HCD1931133.1"
                     /translation="MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLND
                     PENPLLDLVCAKITAWEESAPEFVEFNAMAQAMPGGIAVIRTLMDQYGLTLSDLPEIG
                     SKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID"
     gene            complement(17322..17636)
                     /gene="higB"
                     /locus_tag="NBI74_000759"
     CDS             complement(17322..17636)
                     /gene="higB"
                     /locus_tag="NBI74_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin HigB"
                     /protein_id="HCD1931134.1"
                     /translation="MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAV
                     FPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKCYIREVMTHKEYDFFTAVHRTKG
                     KK"
     gene            complement(17921..19057)
                     /gene="rlmG"
                     /locus_tag="NBI74_000760"
     CDS             complement(17921..19057)
                     /gene="rlmG"
                     /locus_tag="NBI74_000760"
                     /EC_number="2.1.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(1835)-N(2))-methyltransferase
                     RlmG"
                     /protein_id="HCD1931135.1"
                     /translation="MSHLDNGFRSLTLQRFPATDDVNPLQAWEAADEYLLQQLDDTEI
                     RGPVLILNDAFGALSCVLAEHKPYSIGDSYISELATRENLRLNGIDESSVKFLDSTAD
                     YPQQPGVVLIKVPKTLALLEQQLRALRKVVTPDTRIIAGAKARDIHTSTLELFEKVLG
                     PTTTTLAWKKARLINCTFNEPPLADAPQTVSWKLEGTDWTIHNHANVFSRTGLDIGAR
                     FFMQHLPENLEGEIVDLGCGNGVIGLTLLDKNPQAKVVFVDESPMAVASSRLNVETNM
                     PEALDRCEFMINNALSGVEPFRFNAVLCNPPFHQQHALTDNVAWEMFHHARRCLKING
                     ELYIVANRHLDYFHKLKKIFGNCTTIATNNKFVVLKAVKLGRRR"
     gene            19142..19645
                     /locus_tag="NBI74_000761"
     CDS             19142..19645
                     /locus_tag="NBI74_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HCD1931136.1"
                     /translation="MSNLTYLQGYPEQLLSQVRTLINEQRLGDVLAKRYPGTHDYATD
                     KALWQYTQDLKNQFLRNAPPINKVMYDNKIHVLKNALGLHTAVSRVQGGKLKAKAEIR
                     VATVFRNAPEPFLRMIVVHELAHLKEKEHNKAFYQLCCHMEPQYHQLEFDTRLWLTQL
                     SLGQDKI"
     gene            19722..20414
                     /locus_tag="NBI74_000762"
     CDS             19722..20414
                     /locus_tag="NBI74_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin high temperature exclusion protein"
                     /protein_id="HCD1931137.1"
                     /translation="MLRAFARLLLRICFSRRTLKIGCLLLLIAGATIFIADRVMVNAS
                     KQLTWSDVNAVPARNVGLLLGARPGNRYFTRRIDSAAELYHAGKVKWLLVSGDNGRKN
                     YDEASGMQQALIAKGVPAKAIFCDYAGFSTLDSVVRANKVFSENHITIISQEFHNQRA
                     IWLAKQYGIDAIGFNAPDLNMKHGLYTQLREKLARVSAVIDAKILHRQPKYLGPLVMI
                     GPFSEHGCPAKE"
     gene            20493..21479
                     /locus_tag="NBI74_000763"
     CDS             20493..21479
                     /locus_tag="NBI74_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417558.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCD1931138.1"
                     /translation="MIRFAVIGTNWITRQFVEAAHESGKYKLTAVYSRSLEQAQHFAN
                     DFSVEHLFTSLEAMAESDAIDAVYIASPNSLHFSQTQLFLSHKIHVICEKPLASNQAE
                     VDAAIACARENQVVLFEAFKTAYLPNFHLLRQALPKVGKLRKVFFNYCQYSSRYQRYL
                     DGENPNTFNPAFSNGSIMDIGFYCLASAVALFGEPKSVQATASLLASGVDAHGVVVMD
                     YGDFSVTLQHSKVSDSVLASEIQGEAGSLVIEKLSECQKVCFVPRGSQMQDLTQPQHI
                     NTMLYEAELFATLVDEHLVNHPGLAVSRITAKLLTEIRRQTGVIFPADNVKL"
     gene            21762..22727
                     /gene="alx"
                     /locus_tag="NBI74_000764"
     CDS             21762..22727
                     /gene="alx"
                     /locus_tag="NBI74_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family membrane protein Alx"
                     /protein_id="HCD1931139.1"
                     /translation="MNTVGTPLLWGGFAVVVTIMLAIDLLLQGRRGAHAMTMKQAAAW
                     SLVWVTLSLLFNAAFWWYLVQTEGRAVADPQALAFLTGYLIEKSLAVDNVFVWLMLFS
                     YFSVPAALQRRVLVYGVLGAIVLRTIMIFTGSWLISQFDWILYIFGAFLLFTGVKMAL
                     AHEDESGIGDKPLVRWLRGHLRMTDTIDNEHFFVRKNGLLYATPLMLVLILVELSDVI
                     FAVDSIPAIFAVTTDPFIVLTSNLFAILGLRAMYFLLAGVAERFSMLKYGLAVILVFI
                     GIKMLIVDFYHIPIAVSLGVVFGILVMTFIINAWVNYRHDKQRVG"
     gene            23051..24295
                     /gene="sstT"
                     /locus_tag="NBI74_000765"
     CDS             23051..24295
                     /gene="sstT"
                     /locus_tag="NBI74_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HCD1931140.1"
                     /translation="MTTQHSPGLFRRLAHGSLVKQILVGLILGILLAWISKPAAEAVG
                     LLGTLFVGALKAVAPILVLMLVMASIANHQHGQKTNIRPILFLYLLGTFSAALAAVIF
                     SFAFPSTLHLSSSAGDISPPSGIVEVMRGLVMSMVSNPIDALLKGNYIGILVWAIGLG
                     FALRHGNETTKNLVNDMSNAVTFMVKLVIRFAPIGIFGLVSSTLATTGFSTLWGYAQL
                     LVVLVGCMLLVALVVNPLLVWWKIRRNPFPLVLLCLRESGVYAFFTRSSAANIPVNMA
                     LCEKLNLDRDTYSVSIPLGATINMAGAAITITVLTLAAVNTLGIPVDLPTALLLSVVA
                     SLCACGASGVAGGALLLIPLACNMFGISNDIAMQVVAVGFIIGVLQDSCETALNSSTD
                     VLFTAAACQAEDDRLANSALRN"
     gene            complement(24299..24850)
                     /locus_tag="NBI74_000766"
     CDS             complement(24299..24850)
                     /locus_tag="NBI74_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgjV family protein"
                     /protein_id="HCD1931141.1"
                     /translation="MTAYWLAQGVGVIAFLIGITTFFNRDERRFKKQLSVYSAVIGVH
                     FFLLGTYPAGASAILNAIRTLITLRTRSLWVMAIFIVLTGGIGLAKFHHPVELLPVIG
                     TIVSTWALFRCKGLTMRCVMWFSTCCWVIHNFWAGSIGGTMIEGSFLLMNGLNIIRFW
                     RMQRRGIDPFKVEKTPSAVDERG"
     gene            complement(24933..26420)
                     /gene="uxaA"
                     /locus_tag="NBI74_000767"
     CDS             complement(24933..26420)
                     /gene="uxaA"
                     /locus_tag="NBI74_000767"
                     /EC_number="4.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HCD1931142.1"
                     /translation="MQYIKIHALDNVAVALADLAEGTEVSVDNQTVTLRQDVARGHKF
                     ALTDIAKGANVIKYGLPIGYALADIAAGEHVHAHNTRTNLSDLDQYRYQPDFQDLPAQ
                     AADREVQIYRRANGDVGVRNELWILPTVGCVNGIARQIQNRFLKETNNAEGTDGVFLF
                     SHTYGCSQLGDDHINTRTMLQNMVRHPNAGAVLVIGLGCENNQVAAFRETLGDIDPER
                     VHFMICQQQDDEIEAGIAHLHQLYSVMRNDKREPGKLSELKFGLECGGSDGLSGITAN
                     PMLGRFSDYVIANGGTTVLTEVPEMFGAEQLLMDHCRDEATFEKLVTMVNDFKQYFIA
                     HDQPIYENPSPGNKAGGITTLEDKSLGCTQKAGSSVVVDVLRYGERLKTPGLNLLSAP
                     GNDAVATSALAGAGCHMVLFSTGRGTPYGGFVPTVKIATNSELAAKKKHWIDFDAGQL
                     IHGKAMPQLLEEFIDTIVEFANGKQTCNERNDFRELAIFKSGVTL"
     gene            complement(26435..27847)
                     /gene="uxaC"
                     /locus_tag="NBI74_000768"
     CDS             complement(26435..27847)
                     /gene="uxaC"
                     /locus_tag="NBI74_000768"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HCD1931143.1"
                     /translation="MTPFMTEDFLLDTEFARRLYHDYAKDQPIFDYHCHLPPQQIAED
                     YRFKNLYDIWLKGDHYKWRAMRTNGVAERLCTGDASDREKFDAWAATVPHTIGNPLYH
                     WTHLELRRPFGITGKLLSPSTADEIWNECNELLAQDNFSARGIMQQMNVKMVGTTDDP
                     IDSLEHHAEIAKDGSFTIKVLPSWRPDKAFNIEQATFNDYMAKLGEVSDTDIRRFADL
                     QTALTKRLDHFAAHGCKVSDHALDVVMFAEANEAELDSILARRLAGETLSEHEVAQFK
                     TAVLVFLGAEYARRGWVQQYHIGALRNNNLRQFKLLGPDVGFDSINDRPMAEELSKLL
                     SKQNEENLLPKTILYCLNPRDNEVLGTMIGNFQGEGMPGKMQFGSGWWFNDQKDGMER
                     QMTQLAQLGLLSRFVGMLTDSRSFLSYTRHEYFRRILCQMIGRWVEAGEAPADINLLG
                     EMVKNICFNNARDYFAIELN"
     gene            28330..29628
                     /gene="exuT"
                     /locus_tag="NBI74_000769"
     CDS             28330..29628
                     /gene="exuT"
                     /locus_tag="NBI74_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708898.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexuronate transporter ExuT"
                     /protein_id="HCD1931144.1"
                     /translation="MRKIKGLRWYMIALVTLGTVLGYLTRNTVAAAAPTLMEELNIST
                     QQYSYIIAAYSAAYTVMQPVAGYVLDVLGTKIGYAMFAVLWAVFCGATALAGSWGGLA
                     VARGAVGAAEAAMIPAGLKASSEWFPAKERSIAVGYFNVGSSIGAMIAPPLVVWAIVM
                     HSWQMAFIISGALSFIWAMAWLIFYKHPRDQKHLTDEERDYIINGQEAQHQVDTAKKM
                     SVGQILRNRQFWGIALPRFLAEPAWGTFNAWIPLFMFKVYGFNLKEIAMFAWMPMLFA
                     DLGCILGGYLPPLFQRWFGVNLIVSRKMVVTLGAVLMIGPGMIGLFTNPYVAIMLLCI
                     GGFAHQALSGALITLSSDVFGRNEVATANGLTGMSAWLASTLFALVVGALADTIGFSP
                     LFAVLAVFDLLGALVIWTVLQNKPAIEVAQESHNDPAPQH"
     gene            29758..30534
                     /gene="exuR"
                     /locus_tag="NBI74_000770"
     CDS             29758..30534
                     /gene="exuR"
                     /locus_tag="NBI74_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ExuR"
                     /protein_id="HCD1931145.1"
                     /translation="MEITEPRRLYQQLAADLKERIEQGVYLVGDKLPAERFIADEKNV
                     SRTVVREAIIMLEVEGYVEVRKGSGIHVVSNQPRHQQAADNNMEFANYGPFELLQARQ
                     LIESNIAEFAATQVTKQDIMKLMAIQEQARGEQCFRDSEWDLQFHIQVALATQNSALA
                     AIVEKMWTQRSHNPYWKKLHEHIDARTVDNWCDDHDQILKALIRKDPHAAKLAMWQHL
                     ENTKIMLFNETSDDFEFNADRYLFAENPVVHLDTATSGSK"
     gene            30879..31541
                     /locus_tag="NBI74_000771"
     CDS             30879..31541
                     /locus_tag="NBI74_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCD1931146.1"
                     /translation="MELLTQLLQALWAQDFETLANPSMIGILYFVLFVILFLENGLLP
                     AAFLPGDSLLVLVGVLIAKGAMGYPQTILLLTVAASLGCWVSYIQGRWLGNTRTVQNW
                     LSHLPAHYHQRAHHLFHKHGLSALLIGRFIAFVRTLLPTIAGLSGLNNARFQFFNWMS
                     GLLWVLILTTLGYMLGKTPVFLKYEDQLMSCLMLLPVVLLVFGLAGSLVVLWKKKYGN
                     RG"
     gene            31545..31928
                     /gene="mzrA"
                     /locus_tag="NBI74_000772"
     CDS             31545..31928
                     /gene="mzrA"
                     /locus_tag="NBI74_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EnvZ/OmpR regulon moderator MzrA"
                     /protein_id="HCD1931147.1"
                     /translation="MQIPRMSLRQLAWSGAVLLLVSTLLLAWSAVRQQESTLAIRAVH
                     QGTTMPDGFSIWHHLDAHGIPFKSITPKNDTLLITFDSSDQSAAAKAVLDRTLPHGYI
                     IAQQDNNSQAMQWLTRLRDNSHRFG"
     gene            32075..32443
                     /locus_tag="NBI74_000773"
     CDS             32075..32443
                     /locus_tag="NBI74_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1090 domain-containing protein"
                     /protein_id="HCD1931148.1"
                     /translation="MKYRIALAVSLFALSAGSYATTLCQEKEQNILKEISYAEKHQNQ
                     NRIDGLNKALSEVRANCSDSQLRADHQKKIAKQKDEVAERQQDLAEAKQKGDADKIAK
                     RERKLAEAQEELKKLEARDY"
     gene            32481..32786
                     /locus_tag="NBI74_000774"
     CDS             32481..32786
                     /locus_tag="NBI74_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 domain-containing protein"
                     /protein_id="HCD1931149.1"
                     /translation="MSKEHTTEHLRAELKSLSDTLEEVLSSSGEKSKEELSKIRSKAE
                     QALKQSRYRLGETGDAIAKQTRVAAARADEYVRENPWTGVGIGAAIGVVLGVLLSRR"
     gene            32789..33193
                     /locus_tag="NBI74_000775"
     CDS             32789..33193
                     /locus_tag="NBI74_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HCD1931150.1"
                     /translation="MADTHHAQGPGKSVLGIGQRIVSIMVEMVETRLRLAVVELEEEK
                     ANLFQLLLMLGLTMLFAAFGLMSLMVLIIWAVDPQYRLNAMIATTVVLLLLALIGGIW
                     TLRKSRKSTLLRHTRHELANDRQLLEEESREQ"
     gene            33183..33482
                     /locus_tag="NBI74_000776"
     CDS             33183..33482
                     /locus_tag="NBI74_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqjK-like family protein"
                     /protein_id="HCD1931151.1"
                     /translation="MSSKVERERRKAQLLSQIQQQRLDLSASRREWLEATGAYDRRWN
                     MLLSLRSWALVGSSVMAIWTIRHPNMLVRWARRGFGVWSAWRLVKTTLKQQQLRG"
     gene            33669..34061
                     /locus_tag="NBI74_000777"
     CDS             33669..34061
                     /locus_tag="NBI74_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008503138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HCD1931152.1"
                     /translation="MKKLEDVGVLVARILMPILFITAGWGKITGYAGTQQYMEAMGVP
                     GFMLPLVILLEFGGGLAILFGFLTRTTALFTAGFTLLTAFLFHSNFAEGMNSLMFMKN
                     LTISGGFLLLAITGPGAFSIDRLLNKKW"
     gene            34131..35117
                     /gene="yqjG"
                     /locus_tag="NBI74_000778"
     CDS             34131..35117
                     /gene="yqjG"
                     /locus_tag="NBI74_000778"
                     /EC_number="1.8.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionyl-hydroquinone reductase YqjG"
                     /protein_id="HCD1931153.1"
                     /translation="MGQLIDGVWHDTWYDTKSTGGKFQRSASAFRNWLTADGAPGPTG
                     KGGFAAEKDRYHLYVSLACPWAHRTLIMRKLKGLEPFISVSVVNPLMLENGWTFDDSF
                     PGASGDTLYQHEFLYQLYLHADPHYSGRVTVPVLWDKKNHTIVSNESAEIIRMFNTAF
                     DALGAKAGDYYPPALQPKIDELNGWIYDTINNGVYKAGFATSQQAYDEAVAKVFESLA
                     RLEQILGQHRYLTGNQLTEADIRLWTTLVRFDPVYVTHFKCDKHRISDYLNLYGFLRD
                     IYQMPGIAETVNFDHIRNHYFRSHKTINPTGIISIGPWQDLDEPHGRDVRFG"
     gene            35400..35768
                     /locus_tag="NBI74_000779"
     CDS             35400..35768
                     /locus_tag="NBI74_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HCD1931154.1"
                     /translation="MDWYLKVLKNYVGFRGRARRKEYWMFILVNIIFTFVLGLLDKML
                     GWQRAGGEGILTTIYGILVFLPWWAVQFRRLHDTDRSAWWALLFLIPFIGWLIIIVFN
                     CQAGTPGENRFGPDPKLEQE"
     gene            complement(35802..36698)
                     /gene="yhaJ"
                     /locus_tag="NBI74_000780"
     CDS             complement(35802..36698)
                     /gene="yhaJ"
                     /locus_tag="NBI74_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YhaJ"
                     /protein_id="HCD1931155.1"
                     /translation="MAKERALTLEALRVMDAIDRRGSFAAAADELGRVPSALSYTMQK
                     LEEELDVVLFDRSGHRTKFTNVGRMLLERGRVLLEAADKLTTDAEALARGWETHLTIV
                     TEALVPTPAFFPLIDKLAAKANTQLAIITEVLAGAWERLEQGRADIVIAPDMHFRSSS
                     EINSRKLYTLMNVYVAAPDHPIHQEPEPLSEVTRVKYRGIAVADTARERPVLTVQLLD
                     KQPRLTVSTIEDKRQALLAGLGVATMPYPMVEKDIAEGRLRVVSPESTSEIDIIMAWR
                     RDSMGEAKSWCLREIPKLFSGK"
     gene            36803..37504
                     /locus_tag="NBI74_000781"
     CDS             36803..37504
                     /locus_tag="NBI74_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HCD1931156.1"
                     /translation="MITTRTARQCGQADYGWLQARYTFSFGHYFDPKLLGYASLRVLN
                     QEVLAPGAAFQPRTYPKVDILNVILDGEAEYRDSEGNHVQASTGEVLLLSTQPGVSYS
                     EHNLSKDKPLTRMQLWLDACPQRENPLIQKLALNMSKQQLIASPEGGMGSLQLRQQVW
                     LHHIVLDKGESANFQLHGPRAYLQSIHGKFHALTHHEEKAALTCGDGAFIRDEANITL
                     VADSPLRALLIDLPV"
     gene            37527..37691
                     /locus_tag="NBI74_000782"
     CDS             37527..37691
                     /locus_tag="NBI74_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1931157.1"
                     /translation="MSKKSAKKRQPVKPVVAKEPARTAKNFGYEEMLSELEAIVADAE
                     TRLAEDEATA"
     gene            complement(37752..39062)
                     /locus_tag="NBI74_000783"
     CDS             complement(37752..39062)
                     /locus_tag="NBI74_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine dehydratase subunit alpha family protein"
                     /protein_id="HCD1931158.1"
                     /translation="MFDSTLNPLWQRYILAVQEEVKPALGCTEPISLALAAAVAAAEL
                     EGPVERVEAWVSPNLMKNGLGVTVPGTGMVGLPIAAALGALGGNANAGLEVLKDATAQ
                     AISDAKALLAAGKVSVKIQEPCDEILFSRAKVWNGEKWACVTIVGGHTNIVHIETHNG
                     VVFTQQACVAEGEQESPLTVLSRTTLAEILKFVNEVPFAAIRFILDSAKLNCALSQEG
                     LSGNWGLHIGATLEKQCARGLLAKDLSSSIVIRTSAASDARMGGATLPAMSNSGSGNQ
                     GITATMPVVVVAEHFGADDERLARALMLSHLSAIYIHNQLPRLSALCAATTAAMGAAA
                     GMAWLVDGRYETISMAISSMIGDVSGMICDGASNSCAMKVSTSASAAWKAVLMALDDT
                     AVTGNEGIVAHDVEQSIANLCALASHSMQQTDRQIIEIMASKAR"
     gene            complement(39090..40421)
                     /locus_tag="NBI74_000784"
     CDS             complement(39090..40421)
                     /locus_tag="NBI74_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026203.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease"
                     /protein_id="HCD1931159.1"
                     /translation="MEIASNKGVIADASTPAGRAGMSESEWREAIKFDSTDTGWVIMS
                     IGMAIGAGIVFLPVQVGLMGLWVFLLSSVIGYPAMYLFQRLFINTLAESPECKDYPSV
                     ISGYLGKNWGILLGALYFVMLVIWMFVYSTAITNDSASYLHTFGVTEGLLSDSPFYGL
                     VLICILVAISSRGEKLLFKISTGMVLTKLLVVAALGVSMVGMWHLYNVGSLPPLGLLM
                     KNAIITLPFTLTSILFIQTLSPMVISYRSREKSIEVARHKALRAMNIAFGILFVTVFF
                     YAVSFTLAMGHDEAVKAYEQNISALAIAAQFISGDGAAWVKVVSVILNIFAVMTAFFG
                     VYLGFREATQGIVMNILRRKIPAEKINENLVQRGIMIFAILLAWSAIVLNAPVLSFTS
                     ICSPIFGMVGCLIPAWLVYKVPALHKYKGMSLYLIIVTGLLLCVSPFLAFS"
     gene            complement(40699..42063)
                     /gene="tdcG"
                     /locus_tag="NBI74_000785"
     CDS             complement(40699..42063)
                     /gene="tdcG"
                     /locus_tag="NBI74_000785"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HCD1931160.1"
                     /translation="MISAFDIFKIGIGPSSSHTVGPMNAGKSFIDRLESSGLLTATSH
                     IVVDLYGSLSLTGKGHATDVAIIMGLAGNSPQDVVIDEIPAFIELVTRSGRLPVASGA
                     HIVDFPVAKNIIFHPEMLPRHENGMRITAWKGQEELLSKTYYSVGGGFIVEEEHFGLS
                     HDVETPVPYDFHSAGELLKMCDYNGLSISGLMMHNELALRSKAEIDAGFARIWQVMHD
                     GIERGMNTEGVLPGPLNVPRRAVALRRQLVSSDNISNDPMNVIDWINMYALAVSEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFRRPVNERSIARYFLAAGAIGALYKMNASISG
                     AEVGCQGEIGVACSMAAAGLTELLGGSPAQVCNAAEIAMEHNLGLTCDPVAGQVQIPC
                     IERNAINAVKAVNAARMAMRRTSAPRVSLDKVIETMYETGKDMNDKYRETSRGGLAIK
                     VVCG"
     gene            complement(42135..42524)
                     /locus_tag="NBI74_000786"
     CDS             complement(42135..42524)
                     /locus_tag="NBI74_000786"
                     /EC_number="3.5.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enamine/imine deaminase"
                     /protein_id="HCD1931161.1"
                     /translation="MKKIIETQRAPGAIGPYVQGVDLGSMVFTSGQIPVCPQTGEIPA
                     DVQDQARLSLENVKAIVVAAGLSVGDIIKMTVFITDLNDFATINEVYKQFFDEHQATY
                     PTRSCVQVARLPKDVKLEIEAIAVRSA"
     gene            complement(42538..44832)
                     /gene="tdcE"
                     /locus_tag="NBI74_000787"
     CDS             complement(42538..44832)
                     /gene="tdcE"
                     /locus_tag="NBI74_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-ketobutyrate formate-lyase/pyruvate
                     formate-lyase"
                     /protein_id="HCD1931162.1"
                     /translation="MKVDIDTSDKLYADAWLGFKGTDWKNEINVRDFIQHNYTPYEGD
                     ESFLAEATPATTELWEKVMEGIRIENATHAPLDFDTNIATTITAHDAGYINQPLEKIV
                     GLQTDAPLKRALHPFGGINMIRSSFHAYGREMDSEFEYIFTDLRKTHNQGVFDTYSPD
                     MLRCRKSGVLTGLPDGYGRGRIIGDYRRVALYGISYLVRERELQFADLQSRLEKGEDL
                     EATIRLREELAEHRRALLQIQEMAAKYGFDISRPAQNAQEAVQWLYFAYLAAVKSQNG
                     GAMSLGRTASFLDIYIERDFKAGVLNEQQAQELIDHFIMKIRMVRFLRTPEFDSLFSG
                     DPIWATEVIGGMGLDGRTLVTKNSFRYLHTLHTMGPAPEPNLTILWSEALPIAFKKYA
                     AQVSIVTSSLQYENDDLMRTDFNSDDYAIACCVSPMVIGKQMQFFGARANLAKTLLYA
                     INGGMDEKLKIQVGPKTAPLMDDVLDYDKVMDSLDHFMDWLAVQYISALNIIHYMHDK
                     YSYEASLMALHDRDVYRTMACGIAGLSVATDSLSAIKYARVKPIRDENGLAVDFEIDG
                     EYPQYGNNDERVDSIACDLVERFMKKIKALPTYRNAVPTQSILTITSNVVYGQKTGNT
                     PDGRRAGTPFAPGANPMHGRDRKGAVASLTSVAKLPFTYAKDGISYTFSIVPAALGKE
                     DPVRKTNLVGLLDGYFHHEADVEGGQHLNVNVMNREMLLDAIEHPEKYPNLTIRVSGY
                     AVRFNALTREQQQDVISRTFTQAL"
     gene            complement(44866..46074)
                     /gene="tdcD"
                     /locus_tag="NBI74_000788"
     CDS             complement(44866..46074)
                     /gene="tdcD"
                     /locus_tag="NBI74_000788"
                     /EC_number="2.7.2.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionate kinase"
                     /protein_id="HCD1931163.1"
                     /translation="MNEFPVVLVINCGSSSIKFSVLNASDCEVLMSGIADGINSENAF
                     LSVNGGEPAPLAHHSYEGALKAIAFELEKRNLNDSVALIGHRIAHGGSIFTESAIITD
                     EVIDNIRRVSPLAPLHNYANLSGIESAQQLFPGVTQVAVFDTSFHQTMAPEAYLYGLP
                     WKYYEELGVRRYGFHGTSHRYVSQRAHSLLNLAEDDSGLVVAHLGNGASICAVRNGQS
                     VDTSMGMTPLEGLMMGTRSGDVDFGAMSWVASQTNQSLGDLERVVNKESGLLGISGLS
                     SDLRVLEKAWHEGHERAQLAIKTFVHRIARHIAGHAASLHRLDGIIFTGGIGENSSLI
                     RRLVMEHLAVLGVEIDTEMNNRSNSFGERIVSSENARVICAVIPTNEEKMIALDAIHL
                     GKVNAPAEFA"
     gene            complement(46100..47431)
                     /gene="tdcC"
                     /locus_tag="NBI74_000789"
     CDS             complement(46100..47431)
                     /gene="tdcC"
                     /locus_tag="NBI74_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine transporter TdcC"
                     /protein_id="HCD1931164.1"
                     /translation="MSTSDSIVSSQTKQSSWRKSDTTWTLGLFGTAIGAGVLFFPIRA
                     GFGGLIPILLMLVLAYPIAFYCHRALARLCLSGSNPSGNITETVEEHFGKTGGVVITF
                     LYFFAICPLLWIYGVTITNTFMTFWENQLGFAPLNRGFVALFLLLLMAFVIWFGKDLM
                     VKVMSYLVWPFIASLVLISLSLIPYWNSAVIDQVDLGSLSLTGHDGILITVWLGISIM
                     VFSFNFSPIVSSFVVSKREEYEKDFGRDFTERKCSQIISRASMLMVAVVMFFAFSCLF
                     TLSPANMAEAKAQNIPVLSYLANHFASMTGTKTTFAITLEYAASIIALVAIFKSFFGH
                     YLGTLEGLNGLILKFGYKGDKTKVSLGKLNTISMIFIMGSTWVVAYANPNILDLIEAM
                     GAPIIASLLCLLPMYAIRKAPSLAKYRGRLDNVFVTVIGLLTILNIVYKLF"
     gene            complement(47453..48442)
                     /gene="tdcB"
                     /locus_tag="NBI74_000790"
     CDS             complement(47453..48442)
                     /gene="tdcB"
                     /locus_tag="NBI74_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional threonine ammonia-lyase/L-serine
                     ammonia-lyase TdcB"
                     /protein_id="HCD1931165.1"
                     /translation="MHITYDLPVAIDDIIEAKQRLAGRIYKTGMPRSNYFSERCKGEI
                     FLKFENMQRTGSFKIRGAFNKLSSLTDAEKRKGVVACSAGNHAQGVSLSCAMLGIDGK
                     VVMPKGAPKSKVAATCDYSAEVVLHGDNFNDTIAKVSEIVEMEGRIFIPPYDDPKVIA
                     GQGTIGLEIMEDLYDVDNVIVPIGGGGLIAGIAVAIKSINPTIRVIGVQSENVHGMAA
                     SFHSGEITTHRTTGTLADGCDVSRPGNLTYEIVRELVDDIVLVSEDEIRNSMIALIQR
                     NKVVTEGAGALACAALLSGKLDQYIQNRKTVSIISGGNIDLSRVSQITGFVDA"
     gene            complement(48541..49479)
                     /gene="tdcA"
                     /locus_tag="NBI74_000791"
     CDS             complement(48541..49479)
                     /gene="tdcA"
                     /locus_tag="NBI74_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001683315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TdcA"
                     /protein_id="HCD1931166.1"
                     /translation="MSTILLPKTQHLVVFQEVIRSGSIGSAAKELGLTQPAVSKIIND
                     IEDYFGVELVVRKNTGVTLTPAGQLLLSRSESITREMKNMVNEISGMSSEAVVEVSFG
                     FPSLIGFTFMSGMINKFKEVFPKAQVSMYEAQLSSFLPAIRDGRLDFAIGTLSAEMKL
                     QDLHVEPLFESEFVLVASKSRTCTGTATLESLKNEQWVLPQTNMGYYSELLTTLQRNG
                     ISIENIVKTDSVVTIYNLVLNADFLTVIPCDMTSPFGSNQFITIPVEETLPVAQYAAV
                     WSKNYRIKKAASVLVELAKEYSSYNGCRRRQLIEVD"
     gene            49794..50012
                     /gene="tdcR"
                     /locus_tag="NBI74_000792"
                     /pseudo
     CDS             49794..50012
                     /gene="tdcR"
                     /locus_tag="NBI74_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417589.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator TdcR"
     gene            complement(50578..50954)
                     /gene="rnpB"
                     /locus_tag="NBI74_000793"
     ncRNA           complement(50578..50954)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="NBI74_000793"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(50987..52132)
                     /gene="garK"
                     /locus_tag="NBI74_000794"
     CDS             complement(50987..52132)
                     /gene="garK"
                     /locus_tag="NBI74_000794"
                     /EC_number="2.7.1.165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417593.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate 2-kinase"
                     /protein_id="HCD1931167.1"
                     /translation="MKIVIAPDSYKESLSASEVAQAIEKGFREIFPDAQYVSVPVADG
                     GEGTVEAMIAATQGAERHAWVTGPLGEKVNASWGISGDGKTAFIEMAAASGLELVPAE
                     KRDPLVTTSRGTGELILQALESGATNIIIGIGGSATNDGGAGMVQALGAKLCDANGNE
                     IGFGGGSLNTLNDIDISGLDPRLKDCVIRVACDVTNPLVGDSGASRIFGPQKGASEAM
                     IVELDNNLSHYADVIKKALHVDVKDVPGAGAAGGMGAALMAFLGAELKSGIEIVTTAL
                     NLEEHIHDCTLVITGEGRIDSQSIHGKVPIGVANVAKKYHKPVIGIAGSLTNDVGVVH
                     QHGIDAVFSVLTSIGTLDEALRGAYDNICRASRNIAATLAIGMRNAG"
     gene            complement(52229..53119)
                     /gene="garR"
                     /locus_tag="NBI74_000795"
     CDS             complement(52229..53119)
                     /gene="garR"
                     /locus_tag="NBI74_000795"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HCD1931168.1"
                     /translation="MTMKVGFIGLGIMGKPMSKNLLKAGYSLVVADRNPEAIADVIAA
                     GAETAATAKAIAEQCDVIITMLPNSPHVKEVALGENGIIEGAKPGTVLIDMSSIAPLA
                     SREISEALKAKGIDMLDAPVSGGEPKAIDGTLSVMVGGDKAIFDKYYDLMKAMAGSVV
                     HTGEIGAGNVTKLANQVIVALNIAAMSEALTLATKAGVNPDLVYQAIRGGLAGSTVLD
                     AKAPMVMDRNFKPGFRIDLHIKDLANALDTSHGVGAQLPLTAAVMEMMQALRADGLGT
                     ADHSALACYYEKLAKVEVTR"
     gene            complement(53149..53919)
                     /gene="garL"
                     /locus_tag="NBI74_000796"
     CDS             complement(53149..53919)
                     /gene="garL"
                     /locus_tag="NBI74_000796"
                     /EC_number="4.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxyglucarate aldolase"
                     /protein_id="HCD1931169.1"
                     /translation="MNNDVFPNKFKAALAAKQVQIGCWSALSNPISTEVLGLAGFDWL
                     VLDGEHAPNDISTFIPQLMALKGSASAPVVRVPTNEPVIIKRLLDIGFYNFLIPFVET
                     KEEAEQAVASTRYPPEGIRGVSVSHRANMFGTVADYFAQSNKNITILVQIESQQGVDN
                     VDAIAATEGVDGIFVGPSDLAAALGHLGNASHPDVQKAIQHIFNRASAHGKPSGILAP
                     VEADARRYLEWGATFVAVGSDLGVFRSATQKLADTFKK"
     gene            complement(53935..55269)
                     /gene="garP"
                     /locus_tag="NBI74_000797"
     CDS             complement(53935..55269)
                     /gene="garP"
                     /locus_tag="NBI74_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GarP"
                     /protein_id="HCD1931170.1"
                     /translation="MILDTVDVKKKGVHTRYLILLIIFIVTAVNYADRATLSIAGTEV
                     AKELQLSAVSMGYIFSAFGWAYLLMQIPGGWLLDKFGSKKVYTYSLFFWSLFTFLQGF
                     VDMFPLAWAGISMFFMRFMLGFSEAPSFPANARIVAAWFPTKERGTASAIFNSAQYFS
                     LALFSPLLGWLTFAWGWEHVFTVMGVIGFVLTALWIKLIHNPTDHPRMSAEELKFISE
                     NGAVVDMDHKKPGSAAASGPKLHYIKQLLSNRMMLGVFFGQYFINTITWFFLTWFPIY
                     LVQEKGMSILKVGLVASIPALCGFAGGVLGGVFSDYLIKRGLSLTLARKLPIVLGMLL
                     ASTIILCNYTNNTTLVVMLMALAFFGKGFGALGWPVISDTAPKEIVGLCGGVFNVFGN
                     VASIVTPLVIGYLVSELHSFNAALVFVGCSALMAMVCYLFVVGDIKRMELQK"
BASE COUNT        13531 a        14515 c        13844 g        13429 t
ORIGIN      
        1 agtgatttgt agcactgata tatctgtagt ctgataagac gcgcaacctc ttatcaagca
       61 tcattgctca atgccggatg cggcgtgaac gccttatccg gcctacatgt tgagtaaacc
      121 tgcgtttaaa aatcattttt gtacttgcag taatgctcat atcagatata aattagatat
      181 atctaattaa gcaaaaggag gctgatatga gccatcatca cgaagggtgt tgtaaacatg
      241 aaggccagcc acgccatgag ggctgctgca aaggtgagaa gtcagaacac gagcactgcg
      301 gacacggtca ccagcatgaa cacggtcaat gctgcggtgg tcgccacggt cgcggcggcg
      361 gtcgtcggca acgtttcttt ggtcacggtg aattgcgtct ggtgattctg gatattctct
      421 cgcgcgatga cagccacggt tacgagttga ttaaagcgat tgaaaatctg acccagggga
      481 attacacccc aagcccgggc gtcatctacc cgacgctgga ttttctgcag gagcagtcgc
      541 tgattaccat ccgcgaagag gaaggtggta agaagcagat tgcgctgacc gaacaaggcg
      601 cgcagtggct ggaagaaaac cgcgaacagg tagagatgat tgaagaacgc atcaaagcgc
      661 gttgcgttgg cgctgcgctg cgccagaacc cgcaaatgaa gcgggcgctg gataatttta
      721 aagcggtgct ggatttatgc gtcaaccaga gcgatatcac tgatgcacaa ataaaaaaga
      781 tcattgcggt gatcgaccgc gccgcttttg atattaccca actggattaa tcaccgattg
      841 ccggatgcgg cgtaccctac acaccccgta ggcctgataa gatgcgccag catcgcatca
      901 ggcattgtgc tccaaccgcc ggatccggca taccgattaa tgcagcaccg tcaccgcgtc
      961 ttccagtcgg ctggcgcggt gtttcaccat cgccgacgcc tgcgcactct cttccactaa
     1021 ctcggcgttt ttctgggtga tcaggttaag ctcatccact gcacgggtca ggctggaaag
     1081 cccatcggcc tgttccagcg ttgaatggct aatctgagct atcaactggg tgacgttttt
     1141 tacctgcgcc acaatatctt ccatcgtccg tccggcggcg tgtacctgct gcgaaccaga
     1201 ttgcacctta tcagcactgg catcaatcag cttgcgaata tcgttggcag cattggcgct
     1261 gcggctggct aaatgacgca cttcccccgc caccactgca aaacctttgc cctgttcgcc
     1321 ggcacgcgcc gcttccaccg ccgcattcag ggccagaata ttggtctgaa acgcaatatc
     1381 gttaatcagc gaagtaatgg tgccaatgcg ctgggtactg tcggcaatat cgtccattgt
     1441 cttgatcacc gtagtcatcg cctccccgcc ctgcactgcc gcattactgg cggtgataga
     1501 aagtttatcg gcagccgacg ccgtggcaga gttctgtttc accgacgccg ccatttggtt
     1561 catggtcgcc accgtttgct gaacgttatc aactgtctgc tgggtatgtt cgttcagttc
     1621 atcggtgcct ttcgccagcg tctcactgcc gtttctgaca ctggacacct ggcttgagac
     1681 atcattaatt agccaacggc acatcaggcc aagctgccct accgcgcgta atgtcagccc
     1741 cagctcatcg ctgcgcttca gatgctcaac actattacgt tctccggtcg ccactttcag
     1801 tgcctggctg gcgacatttt ctatcggacg tacaatttgc cattcaaaac aggcatttcc
     1861 caacaacatc actaatgcac agaagatata cgtcaccacc ggggcagcaa caaaccagag
     1921 catggccgcc agcacgataa acatcagggt catcactcca cgcgcccgcc agcgaagcgg
     1981 taatgaaggc agtttaccca gccagccttt acgcaccacc aggcctttat gaatacgctt
     2041 accggtacgt ccggcattca gcgctttgta cagcggctcc accgccgcga tctcttcatc
     2101 cgtcgcccgg gtacgaatcg acatatagcc actgattttt ccctcgcgca ccatcggtac
     2161 cgcattggcc cgcacccaat aatggtcgcc atttttgcgg cgatttttca cgatgccgct
     2221 ccagggttcc ccttttttca gggtgaacca catatccgca aacgccgctt ttggcatatc
     2281 cgggtgacgc accatgttgt gcggttgccc ctgtaactcc tgcaaggtat agccgctcac
     2341 ctgcacaaaa gtgtcattag catgagtgat atagctttgc agatcggtag tggacatcag
     2401 agtggtgtcg tccgccagcg gggtattttg ctgggtgaca tacggatgag aagacatgtt
     2461 cgcgtcctgt gcaggttata tggttgttaa cttcttgtca gagtttatgt cggccccgct
     2521 gcggttatct ttaaccaatt aatttgattt agatcgcaat ttgcgattaa agcacaaatc
     2581 taattccttg atttaaaata ctttcactct gttactatac gaaaacgtta attatcttgc
     2641 ccaaaaatca ggcaattatt gccctgaaag cgtgcatttg cgcagcaatc atcaaatcca
     2701 tacccgacaa aaaccgtgca aaataacagc aaatgttagc aaatagcatt aaatattgcg
     2761 caaatgataa ccacatttgt gtttatcccg attttcgcga tcgcagccgg agtggcgcaa
     2821 tccctgcaat acttaaatcg gtatcatgtg atacgcgagc ctccggagca tattttgaac
     2881 aggttacctt cgagcgcatc agctttagcg tgcagcgccc acgccctgaa tctcattgaa
     2941 aagcgaacgt tggatcatga ggagatgaaa gcacttaacc gggaggtgat tgaatacttc
     3001 aaagagcatg tcaatccggg gtttttagag tatcgcaaat ctgttaccgc cggcggggat
     3061 tacggagccg tagagtggca agcgggaggt ttaaatacgc ttgtcgacac ccagggacag
     3121 gagtttatcg actgcctggg aggttttgga attttcaacg tggggcaccg taatccagtt
     3181 gtggtttccg ccgtacagaa tcaacttgcg aaacaaccgc ttcacagcca ggagctgctt
     3241 gatccgttac gggcgatgct ggcgaaaacc cttgcggcgc taacgcccgg taaactgaaa
     3301 tacagcttct tctgtaatag cggcaccgaa tccgttgaag cggcgctgaa gctggcgaag
     3361 gcttatcagt caccgcgcgg caagtttact tttattgcca ccagcggcgc gttccacggt
     3421 aaatcacttg gcgcgctgtc ggccacggcg aaatcgacct tccgcaaacc gtttatgcca
     3481 ctgctgccag ggtttcgtca tgtaccgttt ggcaatatcg aagccatgcg cacggctctt
     3541 aacgagtgca aaaaaaccgg tgatgatgtt gctgcggtga tcctcgaacc gattcagggc
     3601 gaaggtggcg taattctgcc gccgccaggc tatctcaccg ccgtgcgtaa gctgtgcgat
     3661 gagttcggcg cactgatgat cctcgatgaa gtacaaacgg gcatggggcg cacgggcaag
     3721 atgttcgcct gcgagcatga gaacgtacag ccggatatcc tttgtctggc aaaagcgctc
     3781 ggcggcggcg tgatgccgat tggcgcaacc atcgccactg aagaggtgtt ctcagtcctg
     3841 ttcgacaatc cgttcctgca cactaccacc tttgacggca acccgctggc ctgtgcggcg
     3901 gcgctggcga ccatcaatgt gttgctggag cagaacttac cggctcaggc tgaacaaaaa
     3961 ggcgatatgt tgctggacgg tttccgtcaa ctggcgcggg aatatcccga tctggtacag
     4021 gaagcgcgtg gtaaagggat gttgatggcg attgagtttg ttgataacga aatcggctat
     4081 aactttgcca gcgagatgtt ccgccagcgc gtactggtgg ccggaacact caacaacgct
     4141 aaaacgatcc gcattgaacc gccactgaca ctgaccattg aacagtgtga actggtgatc
     4201 aaagcggcgc gtaaggcgct ggcggccatg cgagtcagtg tcgaagaagc gtaatatcaa
     4261 atcggatggc gatacgacgt cgccatccga tctttttata cgatacgtac gcccgcaggc
     4321 atcatccgct ccggcgttaa cagcacgctt tcactgccgt catcggtttc cgcacacaac
     4381 agcatgcatt ccgacgtttc accgcgcatt ttcgcttttt gcagattgca taagaccacc
     4441 actgtttttc ccatcagctc ttcttcgctg tagtacggca ccagactggt cacggtttgc
     4501 agcgtttttt cccccacatc aacctgtacg atgtacagct tgtcggcgtt ttcatggcgt
     4561 ttcacttcca caatctttcc gacgcgcatt tccagacgtg caaaatcagc gtaagccacg
     4621 gtttccattg tttcctccct tcaagtaaaa ttttactaaa ctatagcaaa agtttttctc
     4681 aatcctgtag gctaaaaatg gagaatgcag gcgtgatcac attcgtgagc cgctgtgtta
     4741 ccgtcacagc gtcaaagaaa cgcgctttat ttactgaaaa caggtgaccc gataagcact
     4801 tcctctacaa tgggggcgca catcagggaa agtaaaaaag gtaaacatgg caacactaaa
     4861 agacatcgca atcgaagctg gcgtatccct ggcgacagta tccagggtct tgaatgacga
     4921 tccgacattg aatgtgaaag aagagacgaa acatcgcatt ctcgagatcg ccgaaaagct
     4981 ggagtacaag accagtagcg cccgtaaact ccagacaggc gcagtcaacc aacaccatat
     5041 tctggctatc tacagctacc aacaggagtt ggagatcaac gatccttact atctggcgat
     5101 ccgccacggc attgaaactc agtgcgaaaa gctggcgatc gaactgacca actgttatga
     5161 acacaacggc ttaccagaca ttaaaaacgt caccggtatt ttaattgtcg gcaaacccac
     5221 gtccgccctg cgcgccgccg cctgtgcgtt gaccgacaat atctgtttta tcgactttca
     5281 cgaacccggc agcggttacg atgcggtgga tatcgatctg gcacgtatca gtaaagaaat
     5341 catcgatttc tatatcaatc agggcgttaa tcgtattggt tttattggcg gtgaagactt
     5401 acccggcaag gcggatattc gtgaggtcgc ctttgcggaa tatggtcgtc tgaaacacgt
     5461 ggtacgcgaa gaggatatct ggcgcggcgg tttttccagt tcgtcgggct atgaactggc
     5521 aaaacagatg ctggcgcggg aagactatcc gaaggcactg tttgttgctt ccgattccat
     5581 tgctatcggc gtactgcggg caattcatga acgtggcctg aacatcccac aggatatttc
     5641 gcttatcagc gttaacgata tccccaccgc gcgatttacc tttccgccgc tctccaccgt
     5701 gcgcatccat tccgagatga tgggaagtca gggcgttaac ctggtgtatg aaaaagcccg
     5761 cgatggtcgc gcgctgccgc tgttggtctt cgttcccagc aaattaaaac tgcgcggcac
     5821 gacccgttaa atccccttac acactgtccg gcaatcgttt ttgccggaca gtgctgccgt
     5881 ttattttcgt gatccagtta aagtaaatgc atttacctgc tattttttag taaaaatttt
     5941 actaaactcc ccagcaatta cacaaactac catcaccata aatggttccg atttctctct
     6001 accgggaggc cctatgaatc gctgggaaaa cattcagctc acccacgaaa accgacttgc
     6061 gccgcgtgcg tacttttttt catatgattc tgttgcgcaa gcgcgtacct ttgcccgcga
     6121 aaccagcagc ctgtttctgt ccttaagcgg tcagtggaat ttccactttt ttgaccatcc
     6181 gctgcaagta ccagaagcct tcacctctga gttaatggct gactgggggc atattaccgt
     6241 ccccgccatg tggcaaatgg aaggtcacgg caaactgcaa tataccgacg aaggttttcc
     6301 gttccccatc gatgtgccgt ttgtccccag cgataaccca accggtgcct atcaacgtat
     6361 tttcaccctc agcgacggct ggcagggtaa acagacgctg attaaatttg acggcgtcga
     6421 aacctatttt gaagtctacg ttaacggtca gtatgtgggg ttcagcaaag gcagtcgcct
     6481 gaccgcagag tttgacatca gcgcgatggt taaaaccggc gacaacctgt tgtgtgtgcg
     6541 cgtgatgcaa tgggcggact ctacctacgt ggaagaccag gatatgtggt ggtcggcggg
     6601 gatcttccgc gatgtttatc tgatcggaaa acaactcacg cacattaacg atttcaccgt
     6661 gcgcaccgac tttgacgaag cctattgcga tgccacactt tcctgcgaag tggtgctgga
     6721 aaatctcgcc gcctcccctg tcgtcacgac gctggaatat accctgtttg atggcgaacg
     6781 cgtggtgcac agcagcgcca ttgatcattt ggcaattgaa aaactgacca gcgcccgctt
     6841 tgattttact gtcgaacagc cgcagcaatg gtcagcagaa tccccttatc tttaccatct
     6901 ggtcatgacg ctgaaagacg ccgacggcaa cgttctggaa gtggtgccac aacgcgttgg
     6961 cttccgtgat atcaaagtgc gcgacggtct gttctggatc aataaccgtt atgtgatgct
     7021 gcacggcgtc aaccgtcacg acaacgatca tcgcaaaggc cgcgccgttg gaatggatcg
     7081 cgtcgagaaa gatctccagt tgatgaagca gcacaacatc aactccgtgc gtaccgctca
     7141 ctacccgaac gatccgcgtt tttacgaact gtgtgatatc tacggcctgt ttgtgatggc
     7201 ggaaaccgac gtcgaatcgc acggctttgc taatgtcggc gatatcagcc gtattaccga
     7261 cgatccgcag tgggaaaacg tctacgtcga gcgcattgtt cgccatattc acgcgcagaa
     7321 aaaccatccg tcgatcatca tctggtcgct gggcaatgaa tccggctatg gctgtaacat
     7381 ccgcgcgatg taccatgcgg ctaaggcgct ggatgacacg cgactggtgc actacgaaga
     7441 agatcgcgat gctgaagtgg tcgatattat ttccaccatg tacacccgcg tgccgctgat
     7501 gaatgagttt ggtgaatacc cgcatccgaa gccgcgcatc atctgtgaat atgctcatgc
     7561 gatggggaac ggaccgggcg ggctgacgga gtaccagaac gtcttctata agcacgattg
     7621 cattcaggga cattatgtct gggaatggtg cgaccacggg atccaggcgc aggatgacaa
     7681 cggcaatgtc tggtataaat tcggcggcga ctacggcgac tatcccaaca actataactt
     7741 ctgtcttgat ggtttgatct attccgatca gacgcctggg ccgggcctga aagagtacaa
     7801 acaggttatc gcgccggtaa aaatccacgc gttggatctg actcgcggcg agttgaaagt
     7861 cgaaaataaa ctgtggttta ccacgctgga tgactacacc ctgcacgcag aggtgcgcgc
     7921 cgaaggtgaa acgctcgcga cgcagcagat taaactgtgc gacgttgcac cgaacagcga
     7981 agcccccttg cagatcacgc tgccgcaact ggacgctcgt gaagcgtttc tcaacattac
     8041 ggtgaccaaa gattcccgca cccgctacag cgaagccgga caccctatcg ctacttatca
     8101 gttcccgctg aaggaaaata ccgcgcagcc tgtacctttc gcgccaaata atgcgcgtcc
     8161 gctgacgctg gaagacgatc gtttgagctg caccgttcgc ggctacaact tcgcaatcac
     8221 cttctcaaaa atgagtggca aaccgacatc ctggcaggtg aatggcgagt cgctgctgac
     8281 ccgcgagcca aagatcaact tcttcaagcc aatgatcgac aaccacaagc aggagtacga
     8341 agggctgtgg cagccgaatc atttgcagat catgcaggaa catctgcgcg actttgccgt
     8401 agaacagagc gatggtgaag tgttgatcat cagccgcacg gttattgccc caccggtgtt
     8461 tgacttcggg atgcgctgca cctacatctg gcgcatcgct gccgatggcc aggttaacgt
     8521 ggcgctttcc ggcgagcgtt acggcgacta tccgcacatc attccgtgca tcggtttcac
     8581 catggggatt aacggcgaat acgatcaggt ggcgtattac ggtcgtggac cgggcgaaaa
     8641 ctacgccgac agccagcagg ctaacatcat cgatatctgg cgcagcaccg tcgatgccat
     8701 gttcgagaac tatcccttcc cacagaacaa cggcaaccgt cagcatgtcc gctggacggc
     8761 actgactaac cgccacggca atggtctgct ggttgttccg cagcgcccaa ttaacttcag
     8821 tgcctggcac tacacccagg aaaacatcca cgcagcccag cactgtaacg agctgcagcg
     8881 cagtgatgac atcaccctga atctcgacca ccagttgcct ggcctcggct ccaactcctg
     8941 gggcagcgag gtgctggact cctggcgcgt ctggttccgt gacttcagct acggctttac
     9001 gttgctgccg gtttctggcg gagaagctac cgcgcaaagc caggcgtcgt atgagttcgg
     9061 cgcagggttc ttttccacga atttgcacag cgagaataag caatgaggat catcgataac
     9121 ttagaacagt tccgccagat ttacgcctct ggcaagaagt ggcaacgctg cgttgaagcg
     9181 attgaaaata tcgacaacat tcagcctggc gtcgcccact ccatcggtga ctcattgact
     9241 taccgcgtgg agacggactc cgcgaccgat gcgctattta ccgggcatcg acgctatttt
     9301 gaagtgcatt actacctgca agggcagcaa aaaattgaat atgcgccgaa agagacatta
     9361 caggtagtgg aatattatcg tgatgaaact gaccgcgaat atttaaaagg ctgcggagaa
     9421 accgtagagg tccacgaagg gcaaatcgtt atttgcgata tccatgaagc gtatcggttt
     9481 atctgcaata acgcggtcaa aaaagtggtt ctcaaagtca ccatcgaaga tggttatttc
     9541 cataacaaat aaaaactacg gcggcaaaag gagtttgccg ccaccgctac cctactcatt
     9601 ttcggagatg tgttatgtct gataccaaac gtaatacaat cggcaaattc ggcttgctct
     9661 cgctgacttt tgccgccgtt tacagcttta acaacgttat caataataat attgagcttg
     9721 gactggcctc ggcaccgatg tttttcctcg caacaatttt ttattttatt cccttctgtc
     9781 tgatcatcgc agaatttgtt tcgttaaata aaaactcgga agccggtgtc tacgcgtggg
     9841 taaaaagttc gctgggcgga cgttgggcat ttattactgc ctatacctac tggttcgtaa
     9901 acctgttctt tttcacctca ttattgccgc gcgttattgc ttacgcctcg tatgcattcc
     9961 tcggctacga atatattatg acgccggttg ccaccaccat tatcagtatg gtgctgttcg
    10021 ccttttccac ctgggtttct accaacgggg cgaaaatgct ggggccaatt acctccgtca
    10081 cttcaacgct gatgctgctg ttaacgctct cctacatttt actggcaggt acggcactgg
    10141 ttggcggcgt acagcctgct gaccccatca ctgttgacgc gatgatccca aactttaact
    10201 gggcgttcct cggcgttacc acctggatct ttatggctgc aggtggcgcg gagtccgtcg
    10261 cggtgtacgt taacgacgtc aaaggcggtt cgaaatcgtt cgttaaagtg atcatcctcg
    10321 ccgggatttt tattggcgta ctgtactccg tctcctcggt actgattaac gtcttcgtca
    10381 gcagcaaaga gttgaagttt accggcggat cggtacaggt attccacggc atggcggcgt
    10441 attttggtct accggaagca ctgatgaatc gctttgtcgg tctggtgtcc tttaccgcga
    10501 tgttcggttc cctgctgatg tggacggcaa cgccggtgaa aattttcttc tccgaaattc
    10561 cggaaggcat ctttggtaag aaaaccgtcg aactgaacga aaacggcgtt ccggcgcgcg
    10621 cagcgtggat ccagttcctg atcgtcatcc cgctgatgat tatcccgatg ctcggttcca
    10681 ataccgtgca ggatctgatg aatactatta ttaatatgac tgctgcagcg tccatgcttc
    10741 cgccgttatt catcatgctg gcttacctga atttacgcgc caaattagat cacctgccac
    10801 gcgatttccg tatgggctcc cgccgcaccg gtattatcgt tgtttcaatg ctgattgcga
    10861 tatttgcagt agggtttgtc gcttcgacat tcccgactgg cgcgaatatt ctgaccatca
    10921 ttttttataa cgtcggcggt attgttatct tcctcggctt tgcgtggtgg aaatacagta
    10981 aatatataaa gggattaacg gctgaagagc gccatattga agcgacgcca gccagcaatg
    11041 ttgattaagc agaataataa aaaatgagca ggcattttcc ctcttcctgg atggggagag
    11101 ggaaaatagt ttctgccttt atatttttat aattacaacg ataaaaggct gtactttttc
    11161 tttagctcat ggattaacac aatgaaatta atcactgcac catgcagagc attacttgcc
    11221 ctgccttttt gctacgcttt ttctgcactt ggcgaagaag cacgtccggc agaacatgac
    11281 gacacaaaaa cccccgaaat gatctcgaca tcttctcctt catttcgttt ttacggcgaa
    11341 ttaggggttg gtgggtatat ggatttagag ggtgagaata aacataaata cagcgacggc
    11401 acctatattg aaggtggcct ggagatgaag tacgactcct ggttcggcct gatttacggc
    11461 gaaggctgga ccgtgcaggc cgaccacgac ggtaatgcct gggtgcctga ccatagctgg
    11521 ggtggtttcg aaggtgggat taaccgtttc tatggcggtt atcgtaccaa tgatggcacc
    11581 gaaatcatgc tcagtctgcg tcaggattcc tcgctggatg acctgcaatg gtggggcgat
    11641 ttcacgcccg atctgggcta cgtcattccc aatacccgcg acattatgac tgcgctgaag
    11701 gtacagaact taagcggcaa ctttcgttat agcgttaccg ccacacctgc cggacatcat
    11761 gacgaaagca aagcctggct acattttggc aagtacgatc gctacgacga caaatacacc
    11821 tatccggcaa tgatgaacgg ttacatccag tatgaccttg cagaaggtat cacctggatg
    11881 aacggtctgg aaatcaccga cggcacaggc caactctatc tcacgggcct gcttacccct
    11941 aactttgccg ctcgcgcctg gcaccatacc ggacgcgccg acgggctgga cgtaccggga
    12001 agtgaaagtg ggatgatggt gagcgccatg tatgaagcgt taaagggcgt ttatctctcc
    12061 accgcttaca cctacgccaa acatcgccct gaccacgctg acgatgaaac cacctctttc
    12121 atgcagtttg gtatctggta cgaatacggc ggcggacgtt tcgcaacggc ttttgatagc
    12181 cgcttctaca tgaaaaatgc ctctcacgat cccagcgacc aactcttcct gatgcaatat
    12241 ttttactggt aataaggaca gtaatctcat gaaaatcaaa actattttaa caccagtagc
    12301 ctgtgctctg ctgatgagct tttccgccca tgccgctaac gctgacaatt ataaaaacgt
    12361 gattaaccgt actggcgcgc cgcagtacat gaaggattac gattacgacg atcaccagcg
    12421 ttttaatccg tttttcgatc tcggagcctg gcatggtcat ctgttgccag acggcccgaa
    12481 cactatgggc ggctttccag gcgttgcgtt gttgacggaa gagtacatca actttatggc
    12541 cagcaatttc gaccgcctga ccgtctggca ggacggcaag aaagtcgatt tcacgctgga
    12601 ggcatacagt attcccggcg cgctggtgca aaagttgata tcgaaagatg tacaggtcga
    12661 aatgattctg cgcttcgcca cgccgcgcac atcactactg gaaaccaaaa tcaccagcga
    12721 taaaccgctg gatctggtgt gggatggcga actgctggaa aaactggaag cgaaagaagg
    12781 gaaaccgctt tccgataaaa ccattgctgg cgagtaccct gactatcagc gcaaaatcag
    12841 cgccacccgc gatggcctga aagttacctt tggcaaagtg cgcgccacct gggatctgct
    12901 gacctccggt gaatcagaat atcaggtgca taaatcatta ccgatgcaga ctgaaatcaa
    12961 cggcaatcgc tttaccagta aggcgcatat caacggatcg acgacgctct ataccaccta
    13021 ttcccatctg ctgaccgctc aggaagttag caaagagcaa atgcagatcc gcgatattct
    13081 ggcgcgtccg gcgttttatc tcaccgcctc gcagcaacgt tgggaagaat acctgaagaa
    13141 aggattaacc aatccggatg cgacgccgga acagacgcgc gtcgcggtga aagccatcga
    13201 aacgctcaac ggtaactggc gatcgccggg cggtgcggtg aaatataaca ccgtcacgcc
    13261 gtcggtgacc gggcgctggt tctccggcaa ccagacctgg ccgtgggata cctggaagca
    13321 ggcgtttgcg atggcgcatt tcaatccgga catcgccaaa gagaatatcc gcgcggtctt
    13381 ctcctggcag atccagccag gcgaccgcgt gcgtccgcag gatgtgggct ttgtccccga
    13441 cctgatagcg tggaacctta gccccgagcg tggcggcgat ggcggcaact ggaacgaacg
    13501 taataccaaa cccagccttg ccgcctggtc agtaatggaa gtgtataacg tcacccagga
    13561 taaggcctgg ttggcagaga tgtacccgaa gctggtggcc tatcacgact ggtggttacg
    13621 taaccgcgat cacaacggca acggcgtgcc ggaatatggc gcgacccgcg acaaagccca
    13681 caacaccgag agcggcgaga tgctgtttac ggtgaaaaaa ggcgacaaag aggagacgca
    13741 gtctgggctg aacaactacg cccgtgtggt ggagaaaggc cagtacgaca gtctggaaat
    13801 tccggcacag gttgctgcgt cgtgggaatc aggtcgtgat gacgccgccg ttttcgggtt
    13861 tatcgacaaa gaacagctgg ataaatatgt cgctaacggc ggcaaacgta gcgactggac
    13921 ggtgaaattc gccgaaaacc gcagtcagga cggaacgttg ctgggctact cgctattgca
    13981 ggagtcggtg gatcaggcca gctatatgta cagcgataac cattatctgg cggagatggc
    14041 gacgattctc ggtaagccgg aagaggccaa acgctatcgt cagttagcac aacagctcgc
    14101 ggactacatc aacacttgta tgttcgaccc gactacacag ttctactatg acgtgcgtat
    14161 tgaagataaa ccgctggcga acggctgcgc gggcaaaccg attattgagc gcggtaaagg
    14221 gccggaaggc tggtcgccgc tgtttaacgg tgcggcaacg caggccaatg ccgacgcggt
    14281 ggtgaaggtg atgctggatc cgaaagaatt caacaccttt gtcccgctgg gaacggcggc
    14341 gttaaccaat ccggcttttg gcgcggatat ctactggcgc gggcgcgtat gggtggatca
    14401 gttctggttt ggtctgaaag ggatggagcg ttacggttat cgtgatgatg ccctgaagct
    14461 ggcggatacg ttcttccggc acgccaaagg attaaccgcc gatggcccaa ttcaggagaa
    14521 ttacaacccg ctcactggct cgcaacaagg cgcaccaaat ttctcctgga gtgccgcgca
    14581 tttgtatatg ttgtataacg attttttccg taagcagtaa tttgcaacgg ctggcggatg
    14641 cggcgtgaac gccttatccg ccctacatgt gcaatccccc aggccggata aaacgcggca
    14701 agcgtcgcat ccggcgttac caccgggcgt attctttttg aatcccatca caaaccccgc
    14761 actccccttt tcccttttct ccggcgacgg ctaaattaga actcatccga ccacataaca
    14821 attattttac atactggaca attttatgag ctacccgtcg ctgttcgccc cgctggattt
    14881 aggttttacc acgttaaaaa accgcgtgtt gatgggctca atgcacaccg ggctggagga
    14941 gtacccggac ggcgctgaac ggctggcagc gttttatgcc gaacgcgccc gtcacggcgt
    15001 ggcgttgatt gtcagcggcg gtatcgcacc agatttaaca ggcgttggca tggaaggcgg
    15061 tgcaatgctc aatgacgcca accagatccc acaccatcgc accattaccg aagccgtaca
    15121 tcaggaaggc ggcaaaatcg ccctgcaaat tttgcatacc gggcgctaca gctaccaacc
    15181 gcatctggtc gctccctccg cattgcaggc ccccatcaac cgtttcgttc ctcacgaact
    15241 cacccatgaa gagatcctgc aactgatcga cgatttcgcc cactgcgcgc aattggcacg
    15301 ggaggcagga tacgacggtg tagaggtgat ggggtccgaa gggtatttga tcaacgaatt
    15361 tctgacgctg cgcaccaatc agcgtagtga ccagtggggc ggcgattacc gcaaccggat
    15421 gcggtttgcc gtagaagtag tgcgtgcggt gcgcgaacgt gtcggcaacg acttcattat
    15481 tatctaccgt ttgtcgatgc tcgacctggt cgaaggcggc ggtacttttg ccgaaatggt
    15541 agagctggcg caggccattg aagcggcggg cgcgaccatt atcaacaccg gcattggctg
    15601 gcatgaagcg cggattccga ccatcgccac gcccgtgccg cgcggcgcct ttagctgggt
    15661 cacgcgcaaa ctgaaagggc acgtttcgct gccactggtg accaccaacc ggattaacga
    15721 tccgcaggtt gccgacgaca ttctctcgcg cggtgatgcg gacatggtat cgatggcgcg
    15781 accgtttctc gctgatgcgg agctgctatc aaaagcgcaa tcgggacgag ccgatgagat
    15841 caacacttgt attggctgca accaggcctg tctcgatcag atcttcgttg gcaaagtcac
    15901 ctcgtgcctg gtgaatcctc gcgcctgcca cgaaaccaaa atgccaatcc ttcctgccgt
    15961 gcagaaaaaa aatctggcgg tggtcggtgc gggacctgct gggctggcgt ttgccattaa
    16021 cgcggcggcg cgtgggcatc aggtaacatt gtttgatgcg cacagcgaga tcggcgggca
    16081 gtttaatatc gccaaacaga tccccggcaa agaggagttt tacgaaacgc tgcgctatta
    16141 ccgccggatg atcgaagtga cgggtgtaac gctaaaactc aatcacaccg tgacggcgga
    16201 tcagttacag gcgttcgatg aaacgatcct cgccagtggg atcgtgccgc gcgttccgcc
    16261 tatcgacggg atcgatcatc cgaaggtact gagttatctc gatgtactgc gcgacaaagc
    16321 gccggtgggc aacaaagttg ccatcatcgg ttgtggcgga attggttttg atacggcgat
    16381 gtatttaagt cagccgggcg aatccaccag ccagaatatc gccgggttct gtaatgagtg
    16441 gggaatcgac agtagcctgc aacaggccgg aggtttgagt ccgcaaggca tgcaaatccc
    16501 ccgtagccca cggcagattg tgatgctcca gcgcaaagcc agcaaaccag gacaagggtt
    16561 aggcaaaacc accggctgga tccatcgcac caccctgctc tcacgcggcg tgaaaatgat
    16621 cccaggcgta agttaccaga agatcgacga tgacgggctg catgtagtga tcaacggcga
    16681 aacgcaggta ctagcagtgg acaatgtggt gatctgcgcg gggcaagagc caaaccgcgc
    16741 attggcgcaa ccgctgattg atagtgggaa aactgtgcat ttaattggcg gctgcgatgt
    16801 agctatggag ctggacgcac ggcgggcaat tgcccaggga acacggctgg cgctggagat
    16861 ttaaatcgtt gtttgaagcg ccggatgcga cgcatccggc acgtcgcgtt aatcaataaa
    16921 caaggcggga gaaataccga atcgcgttgc cagtttttta gcgtgttcca gcgttaattt
    16981 ccttttcccg ctcaaaacgc gtgacaccat cgatttactg cctatttccg gaagatcgga
    17041 aagggttaaa ccatattgat ccataagggt acgaatcacg gctataccgc caggcatggc
    17101 ttgagccatg gcattaaatt ccacaaattc gggcgctgat tcttcccacg cggttatttt
    17161 ggcacacacc agatccagta aggggttttc aggatcgttg agcagcagat gatctaccag
    17221 ttccagcgcc tgggtgtatt gttcctcgtt ctgaataccc gccagaaaag gtgccacagc
    17281 agttagcttt tctcctgctt gcaagatgtc ggcaatcgca atcatttttt ccccttagta
    17341 cgatgaacgg cggtaaagaa atcgtattct ttatgcgtca taacttcacg tatgtagcac
    17401 ttttgcgatt caaaaaagac cattgctaca acacgtaatt cattgccccc gacattgaaa
    17461 acataatgct tatccagata tttgaagtta tccagagaag ggaatactgc ttttaatgac
    17521 tcaggttttt tgaaatatcc cttagcaatc gtgttcccca gagccaccaa ctccgtttta
    17581 tgttgcgggt atttttccgc agcatctttc aatgcttttt gagttatcag gtgcattctt
    17641 catcacgtcc gttgacaaat tggcaatatg ataacatccg ttgacagatt ggcaacgaat
    17701 gaatttatct gtgtgacgga agaataaacg tagaaaaacg ctaaacaaga gaatgtagag
    17761 gatggtgaca ggaatttttt gttttatgga agcgcgattc cagaactggg cagacattgc
    17821 cggatgcgac gcttgccgcg tcttatccga cctgggggta atgtgctgaa cgtatgccgg
    17881 ataaggcgtt tacgccgcat ccggcaggta acccccaatc ttaccgacga cgccccagct
    17941 tcactgcttt cagcaccaca aatttattat tcgtggcgat ggtggtgcag ttgccgaaaa
    18001 ttttcttcag tttatggaag taatccaagt gacggttggc aacgatatac agctcgccgt
    18061 tgattttcag gcagcggcgg gcgtggtgga acatctccca ggcgacgtta tcggtcagcg
    18121 catgttgctg atgaaacggc gggttgcaga gcacagcatt aaaacggaaa ggctccacgc
    18181 cggaaagcgc gttgttgatc ataaactcgc agcgatccag tgcctcgggc atgttggttt
    18241 caacgttcaa acggctggaa gcaaccgcca tcggtgattc atcgacaaac accactttcg
    18301 cctgcgggtt tttatcgagc aatgtcaggc caataacgcc attaccgcaa ccgagatcga
    18361 caatctcacc ttcgagattc tctggcagat gttgcataaa gaagcgcgcg ccaatatcca
    18421 gcccggtgcg ggagaagaca ttcgcatggt tgtggatagt ccagtcagta ccttccagct
    18481 tccagctaac ggtctgcggc gcatcggcca acggcggttc gttgaaggtg cagttaatca
    18541 ggcgcgcttt cttccatgcc agcgtggtgg tggtcgggcc gagcactttt tcgaacaatt
    18601 ccagcgtgga agtgtgaatg tcacgggctt tggcaccagc aataatgcgc gtatctggcg
    18661 tgaccacttt gcgcagcgcg cgcagttgtt gttccagcaa tgccagtgtt ttcggcactt
    18721 tgatcagcac cacacccggc tgttgcgggt agtcggcggt gctgtcgaga aacttcacgc
    18781 tcgactcatc aatcccgttg aggcgtaaat tctcgcgcgt ggccagttca ctgatgtatg
    18841 agtcgccaat gctgtacggc ttatgttccg ccagcacaca gcttaacgca ccaaaagcat
    18901 cattcaggat caacaccggg ccgcggattt ctgtgtcgtc caactgttgc agcaaatact
    18961 catccgccgc ttcccacgcc tgtagcgggt taacgtcatc cgtcgccgga aaacgttgta
    19021 gtgtcagtga acggaaaccg ttgtctaagt ggctcatcgg ccctcctgaa tgataaaatt
    19081 tcgacgttgc ccctgaaaag ggtgcgtgag tatacccttt ttctcttttt cgtgggcggt
    19141 tatgagcaat cttacttatc ttcagggtta tcccgagcag ctactttccc aggtgcgaac
    19201 gctgattaac gaacagcgtc tgggcgatgt gctggcaaaa cgttatccgg gaacacacga
    19261 ctacgccacc gataaagccc tctggcaata tactcaggat cttaaaaatc agtttctgcg
    19321 caatgccccg ccgatcaata aagtgatgta tgacaataag atccacgtgc tgaaaaacgc
    19381 gctcgggtta catactgccg tttcgcgtgt gcagggcggc aagctgaaag caaaagcaga
    19441 gatccgcgtc gctaccgtgt ttcgcaacgc gccggaaccg tttttgcgca tgatcgtggt
    19501 gcacgagctg gcgcacctga aggagaaaga gcataacaaa gcgttttacc agttgtgctg
    19561 tcatatggaa ccgcagtacc accagcttga gttcgacacc cggctgtggc taacgcagtt
    19621 gtcgcttggg caggacaaaa tctgaaaatg atgtcaggcg ttattttttc ctcctttagc
    19681 gcctaaaatc gacctcctcc ctttcgttaa ggataacgac catgctgcgc gcatttgccc
    19741 gccttcttct tcgtatttgt ttttctcgcc ggactcttaa aattggctgt ttattgttgc
    19801 ttatagcagg agcgaccatt tttattgccg atcgcgttat ggtcaacgcc agcaagcagc
    19861 tgacctggag tgatgtcaac gccgttccgg cgcggaacgt gggtttattg ctgggggcga
    19921 ggccgggtaa tcgctacttt acccgacgta ttgatagcgc cgcagagttg taccacgccg
    19981 gaaaagtgaa atggctattg gtgagcggcg ataatggacg taaaaattac gatgaagcgt
    20041 caggaatgca gcaagccttg atcgctaaag gcgtaccggc aaaagccatc ttctgcgact
    20101 atgccggatt ctcaacgctt gattcggtgg tgcgtgccaa caaggtgttt agtgaaaacc
    20161 atatcaccat tatctcgcaa gaatttcata accaacgcgc catctggctg gcaaaacagt
    20221 atggtatcga tgctatcggt tttaacgcgc cagacctgaa tatgaagcac ggtttatata
    20281 ctcaactgcg ggaaaaactt gccagagtca gcgcggtgat tgatgcgaaa atcctccatc
    20341 gccagccgaa atatctcggg ccgttggtta tgattgggcc gtttagtgag catggctgtc
    20401 cggcaaaaga ataatgcgta tctgcgcacg tcgaagataa aaaaggcgtg ctacagtgac
    20461 gacagaatcc ctttatggag tatccacgcg ttatgatacg tttcgctgtg attggtacga
    20521 actggatcac tcgccagttc gtcgaggccg cccatgagag cggtaaatac aagttaaccg
    20581 ccgtctattc ccgcagcctt gaacaggccc agcacttcgc caatgatttt tctgtcgagc
    20641 atctgtttac gtcgctggaa gcgatggcag aaagcgatgc cattgacgcg gtgtatattg
    20701 ccagcccaaa ttccctgcac ttttcccaga cacaactttt ccttagtcac aaaattcatg
    20761 tgatttgcga gaagccactg gcgtcgaatc aggcggaagt ggatgccgcc attgcctgtg
    20821 cgcgggaaaa tcaggtggtg ctgtttgagg catttaaaac ggcctacctg ccgaactttc
    20881 atctgttacg ccaggcgctg ccgaaagtcg gcaaactgcg taaagtcttt ttcaactatt
    20941 gccagtattc ctcgcggtat cagcgttacc tggacggaga gaatcccaac accttcaatc
    21001 cggcattctc caacggttcg attatggata tcggctttta ctgcctggcg tcggcggtgg
    21061 cattattcgg tgaaccgaaa agcgtgcagg caaccgccag tttgctggca agcggtgtgg
    21121 atgcccacgg cgtggtggtg atggattacg gtgatttcag cgttacgttg cagcactcaa
    21181 aagtgagcga ttctgtcctg gcaagcgaga ttcagggcga agcgggatcg ctggtgattg
    21241 aaaaactctc tgaatgccag aaagtctgct ttgtaccgcg tggcagccag atgcaggatc
    21301 tcacccagcc acagcatatt aataccatgc tctacgaagc agagctgttc gctaccctgg
    21361 tggatgagca tctggtgaat catccagggc tggcggtcag tcgtatcacc gccaaactgc
    21421 tgactgagat ccgccgccag actggggtga tttttccggc agataacgta aaactataat
    21481 tgccaaagta aaacagtgta aaaggtatgt aacagaccat tgactgactg aatggtctgt
    21541 catactttgt tacctgcaaa ggggagtaac ttcattgtcg gtcgatcgtc attacggtgt
    21601 gtgaaaaacc acatccggtc accgggcaac ccgaaaggaa tacgcagacg tattcctttc
    21661 gggttgtaag tgagaccttg ccggaaggcg aggtctatgc ataaaaagca gcggctgacg
    21721 tcttccgacg ttggccgttt ttttatgtgt aaggaacttc tatgaatact gtcggcacgc
    21781 cgttgctatg gggcggattc gctgttgttg tcaccattat gctggctatc gacctgttgt
    21841 tgcaggggcg tcgtggggca catgccatga ccatgaaaca ggctgcggcc tggtcgctgg
    21901 tctgggtgac gctgtcgtta ctgtttaacg ccgctttctg gtggtatctg gtgcaaaccg
    21961 aaggtcgcgc cgttgccgat ccacaggcac tggcctttct cacgggttat ctgattgaga
    22021 aatcgctggc ggtcgataac gtctttgtct ggctgatgtt gttcagctat ttctctgttc
    22081 cggcggcatt acaacgccgc gtgctggtgt atggcgtact cggggcgatt gttctgcgta
    22141 ccatcatgat cttcactggt agctggctga tttcacagtt cgactggatc ttgtatatct
    22201 tcggtgcctt cctgctgttt accggcgtga agatggcgct cgcccatgaa gatgaatcag
    22261 gcattggcga caagccgctg gtgcgctggc tgcgcggtca tttgcgcatg accgacacca
    22321 tcgacaacga gcatttcttt gtgcgtaaga atggtttgtt gtatgccacg ccgctgatgc
    22381 tggtgctgat tctggtggag ttgagcgacg tgattttcgc cgtggatagt attccggcta
    22441 tcttcgccgt gaccaccgat ccgtttattg tgctgacctc aaacctgttt gcgatcctcg
    22501 gcctgcgtgc gatgtatttc ctgctggcag gtgtggcaga gcgtttctcg atgctcaaat
    22561 atggtctggc ggtgattctg gtgtttatcg gtatcaagat gctgattgtc gacttctacc
    22621 atattccgat cgccgtctcg ctgggcgtgg tgtttggcat tctggtgatg acgtttatta
    22681 tcaacgcctg ggtgaattat cggcatgata agcagcgggt tggatgattt ttaatctgcc
    22741 taagccgtgt accctgtcat caacatgagc accgttttct ccctctcccc aaaaggggcg
    22801 aggggactga ccgagtactt ttttgacgtt gtcatcagtc tggaagccgc acgttgggct
    22861 ttattttcat gtcaaacaaa tgtaaccatt aagtttcgaa atatgacttc tctttaaaat
    22921 ccagcatatt tcgcttcccg aagctgtaac tttccttata ctcgaccttg caaacacttt
    22981 gttacatcct gaaagatgcg tcgacagaac gcaccaggga tgtgcgacaa cacaatgaaa
    23041 ggatcgaaaa atgactacgc aacattcacc ggggctattc cggcgtctgg ctcatggcag
    23101 cctggtaaaa caaatcctgg tcggccttat tctggggatt cttctggcat ggatctcaaa
    23161 acccgcggcg gaagctgttg gtctgttagg tactttgttc gtcggcgcac tgaaagccgt
    23221 tgcccccatc ctggtgttga tgctggtaat ggcatctatt gctaaccacc agcacgggca
    23281 gaaaaccaat atccgcccta ttttgttcct ctatctgttg ggtaccttct ctgctgctct
    23341 ggccgcagta atcttcagtt ttgccttccc ttctaccctg cacttgtcca gtagcgcggg
    23401 tgatatttcg ccgccgtcag gcattgtaga agtgatgcgc gggctggtaa tgagcatggt
    23461 ttccaacccc attgatgcgc tgctgaaagg taactacatc gggatcctgg tgtgggcaat
    23521 tggcctcggt ttcgcactgc gtcacggtaa cgaaaccacc aaaaacctgg tcaacgatat
    23581 gtcgaatgcc gtcaccttta tggtgaaact ggtgattcgc ttcgcaccga tcggtatttt
    23641 tggtctggtt tcttctaccc tggcaaccac cggtttctcc acgctgtggg gctacgcgca
    23701 actgctggtc gtgttggttg gctgtatgtt actggtggcg ctggtggtta accccttgct
    23761 ggtgtggtgg aaaattcgtc gtaacccgtt cccgctggtg ctgctgtgcc tgcgcgaaag
    23821 cggcgtgtat gccttcttca cccgcagctc tgcggcgaac attccggtga atatggcgct
    23881 gtgtgaaaag ctgaatctgg atcgcgatac ctattccgtt tctattccgc tgggagccac
    23941 catcaatatg gcgggcgcag caatcaccat taccgtgttg acgctagctg cagttaatac
    24001 gctgggtatt ccggttgatc tgcccacagc gctgctgttg agcgtagtgg cttctctgtg
    24061 tgcctgtggc gcatccggcg tggcgggggg ggctctgttg ctgattccac tggcctgtaa
    24121 tatgttcggt atttcgaacg atatcgccat gcaggtggtt gctgtcggct ttattatcgg
    24181 cgtattgcag gactcctgtg aaaccgcgct gaactcgtca actgacgtgc tgttcactgc
    24241 ggcagcttgc caggcggaag acgatcgtct ggcaaatagc gccctgcgta actaatactt
    24301 agcccctttc gtctacggcg gaaggggttt tctccacttt aaacggatca attccccttc
    24361 tctgcatacg ccagaaacga atgatattca ggccattcat cagcagaaaa ctgccttcaa
    24421 tcatcgtgcc gcctatcgac cccgcccaga agttgtgaat cacccagcaa cacgttgaaa
    24481 accacattac gcagcgcatg gtcagccctt tacagcggaa cagcgcccag gtactgacaa
    24541 tcgtgccgat taccggcaat agttcaacag gatgatggaa cttcgcgagg ccaattccgc
    24601 cagtcagcac aataaaaatc gccattaccc ataagctgcg cgtgcgtaag gtaatcaatg
    24661 tacgaatggc attaaggatg gcactggcac cggcaggata ggtgcccaga agaaaaaaat
    24721 gtacgccaat aacggcgcta tagaccgaaa gttgcttttt gaagcgacgt tcgtcacgat
    24781 tgaaaaatgt tgtgatacca atcagaaagg cgatgacacc cacgccctgg gccagccaat
    24841 acgcggtcat gataaatcct tagcaggtat ggaaaagcaa acggcgcttc acattatgaa
    24901 acgccgtttt ttattaacaa ctcatttcga ctttatagcg ttacgccgct tttaaagatc
    24961 gccagttcgc ggaagtcgtt acgctcgtta caggtttgct taccgttggc aaactcaacg
    25021 atggtgtcga taaattcttc cagcaactgc ggcatcgctt taccgtggat cagctgaccc
    25081 gcgtcaaagt cgatccagtg ttttttcttc gccgccagtt cactgttggt ggcgattttc
    25141 accgtcggca caaatccacc atacggcgtg ccacgaccag tactgaacag caccatatgg
    25201 cagcccgcac ccgccagggc gctggtcgct acggcatcgt tacccggcgc acttaacaag
    25261 ttcagccccg gcgttttcag acgctcgccg taacgcagca cgtcaaccac gacactggaa
    25321 cccgctttct gggtacagcc aagcgattta tcttctagcg tggtgatacc gcctgctttg
    25381 ttccccggtg atgggttttc gtagatcggc tggtcatggg caataaagta ctgcttgaag
    25441 tcattgacca tggtgaccag tttttcaaac gttgcttcgt cgcggcaatg gtccatcagc
    25501 aactgctctg cgccaaacat ctccggcact tcggtcagta cggtagtacc gccgttagca
    25561 atcacgtagt cagagaaacg ccccagcatc gggttagcag taataccaga aagaccgtca
    25621 gaaccaccgc actccagacc aaacttcagt tcgctgagtt tgcccggctc gcgtttatcg
    25681 ttgcgcatca cgctatacag ctgatgcaaa tgcgcgatcc cggcctcgat ttcatcatcc
    25741 tgctgttggc agatcatgaa atgaacgcgt tcaggatcga tatcgcccaa cgtttcacgg
    25801 aatgcggcaa cctggttgtt ttcacagccc agaccaatca ccagcactgc gcccgcgttc
    25861 gggtggcgca ccatgttttg cagcatggtg cgagtattga tgtgatcgtc gcccagttgt
    25921 gagcagccgt aggtgtggct gaagaggaac acgccatcgg taccttcagc gttgttggtc
    25981 tctttcagga aacgattttg gatctgccgc gcgatgccgt tgacacagcc cacggttggc
    26041 aggatccaca gctcattacg caccccgaca tcgccgttag cgcgacgata gatctgcact
    26101 tcacgatctg ccgcttgcgc aggcagatcc tgaaaatcag gttgatagcg atactgatcc
    26161 agatcgctca gattcgtgcg cgtattgtgg gcgtgaacgt gttcccccgc cgcaatatcc
    26221 gccaatgcat aaccaatcgg caggccatat ttaatgacgt tggccccttt tgcgatatcc
    26281 gttaacgcaa atttatgtcc acgagcaaca tcctggcgca gcgtaacagt ctggttatcg
    26341 acactgactt ctgtgccttc agccaaatcc gctaaagcaa ccgcgacgtt atccagcgca
    26401 tggatcttga tgtattgcat atcaacccca gaccttagtt cagttcaatg gcgaagtagt
    26461 cacgcgcatt gttaaagcaa atatttttca ccatctcgcc cagcaggttg atgtccgccg
    26521 gtgcttcgcc tgcttccacc cagcgaccga tcatctggca cagaatgcgg cggaagtatt
    26581 cgtgacgggt gtatgacagg aagctacggc tgtcagtcag catgccgaca aagcggctca
    26641 gcagaccgag ctgcgccagt tgggtcatct gacgttccat accgtctttc tggtcgttaa
    26701 accaccagcc ggaaccgaac tgcattttgc ccggcatacc ttcgccctgg aagttaccga
    26761 tcatggtgcc cagcacttcg ttatcgcgcg ggttcaggca gtagagaatg gttttcggca
    26821 gcaggttttc ttcgttctgc ttgctcagca gcttagatag ctcttccgcc atcggacggt
    26881 cgttgatgga gtcaaagccg acatccggcc ccagcagttt gaactgacgc aggttgttat
    26941 tacgcagtgc gccaatgtgg tactgctgta cccatccgcg acgtgcatat tcggcaccaa
    27001 ggaacaccag caccgcagtt ttgaactgtg ccacttcgtg ctcgctcagg gtttcgcctg
    27061 ccagacggcg cgcgaggatg ctgtccagtt ccgcttcgtt cgcttcagca aacatcacta
    27121 catccagcgc gtggtcagac actttacagc cgtgagcggc gaagtgatcc agacgtttag
    27181 tcagggcagt ttgcaggtca gcaaagcggc gaatgtcggt atcagaaact tcgcccagct
    27241 tcgccatgta gtcgttaaag gtcgcctgtt caatgttaaa ggctttgtcc ggacgccagc
    27301 tcggcagcac tttaatggtg aaagagccgt ctttggcgat ctctgcgtga tgctccagag
    27361 aatcgatcgg gtcatcggtg gtgccgacca ttttcacgtt catctgctgc atgatgccgc
    27421 gcgcggagaa gttatcctgc gccagcagtt cgttacattc gttccagatt tcatcggcag
    27481 ttgacggaga aagcaatttg ccagtgatac caaacggacg acgcagttcg aggtgcgtcc
    27541 agtggtataa cgggttgcca atagtgtgcg gaacagtcgc cgcccaggcg tcaaattttt
    27601 cacggtcaga cgcatcaccg gtgcacagac gctcggccac accgttggta cgcatagcgc
    27661 gccatttgta gtgatcgccc ttcaaccaga tgtcatacag gtttttaaaa cgatagtctt
    27721 ccgcaatctg ctgcggcggc aaatggcaat ggtaatcgaa aatcggctgg tcttttgcgt
    27781 agtcgtgata cagacggcgg gcaaattcag tatctaacag gaaatcttca gtcataaacg
    27841 gagtcatttt cgtcttcctc tcaacgagtg agctagcttg cttatggtgc gatgctgaca
    27901 aagttatcac accaatttcc agagtccgaa gatattttcg tgagttagat caataaacgt
    27961 agttaaaaaa attactctca aagtggtaaa tctcgctgca ggccgcgcca gtactggcct
    28021 cgccgtcgtc aggtaatgtc cctacaaata ttcgcacatt tgtgatggct ctcacctttt
    28081 aaagttgtat gacaagttat ctttctgccg tcgcaaatca taagtcgacg gaatgcaaat
    28141 tgccgattca ttcatttgtt agatgaatcg ggttaaccgg tacggaagcc gaattagcac
    28201 gaaactttca tggcaacgtt cggggcgtgc cggttttttt tcggttaccc ggtcgtaact
    28261 aacatcttca gcctctggcg ggatgatggc cgcgcttcct gcggatataa caaaacgatg
    28321 aggttttaca tgcgtaaaat taaagggtta cgttggtata tgatcgcact ggtgacgctc
    28381 ggcaccgtgc ttggttacct gacgcgtaac actgtggcgg cagctgcgcc aactctgatg
    28441 gaagagttaa acatctccac ccaacagtat tcctatatca tcgcagcgta ttctgctgct
    28501 tatacggtca tgcaaccggt agcgggttat gtactggatg tgctgggtac gaaaatcggt
    28561 tatgcaatgt ttgctgtact gtgggccgtg ttctgtggtg caaccgcgct ggcaggtagt
    28621 tggggtggcc tggcggttgc tcgtggtgcg gtcggtgccg cggaagccgc gatgattccg
    28681 gcgggtctga aagccagttc cgaatggttc ccggcaaaag agcgttccat cgcggtaggt
    28741 tactttaacg taggttcttc gattggtgcg atgattgcgc caccgctggt ggtatgggca
    28801 atcgtgatgc acagctggca gatggcattt atcatctccg gtgcgttgag ctttatctgg
    28861 gcgatggcat ggctgatttt ctataaacat ccgcgcgacc agaaacatct gaccgatgaa
    28921 gaacgcgact acattattaa tggtcaggaa gcccagcacc aggttgacac agcgaagaaa
    28981 atgtccgttg gtcagatcct gcgtaaccgt cagttctggg gtatcgcgct gccgcgattc
    29041 ctggcagaac cggcatgggg tacttttaac gcgtggatcc cgctgttcat gtttaaagtt
    29101 tacggcttta acctgaaaga gatcgcgatg tttgcctgga tgccgatgct gtttgctgac
    29161 ctcggttgta tcctcggtgg ttacctgccg ccgctgttcc agcgttggtt tggtgtgaac
    29221 ctgatcgttt cccgtaagat ggtcgtaacg ctgggtgcgg tgctgatgat tggcccgggt
    29281 atgatcggtc tgttcaccaa cccgtatgtc gcgattatgc tgctgtgtat cggtggtttt
    29341 gctcaccagg cactgtctgg cgcactgatt acgctctcgt ctgacgtgtt cggtcgtaac
    29401 gaagtggcga cggcgaacgg tttaaccggg atgtccgcat ggctggcaag tacgctgttt
    29461 gcactggtag tcggtgcact ggctgacacc atcggcttca gcccgctgtt cgcagttctg
    29521 gcagtgttcg acctattagg tgcgctggtt atctggaccg tgttgcagaa caaaccggcg
    29581 attgaggtgg cgcaggaatc acataacgat cccgcaccgc aacattaagg cttcgtgtaa
    29641 gttttagttt cccttcaaag ccgccttctc aggcggcttt ttcatcactg cgagtagagc
    29701 taaactcgca aaagtggtat aacaaatata gtctgccgta tcatgcctgg agcgcatatg
    29761 gaaatcactg aaccacgccg tttgtatcaa caacttgccg ctgatctgaa agagcgcatc
    29821 gaacagggcg tctatctggt aggtgataaa ctgcctgccg aacgctttat tgccgatgaa
    29881 aaaaacgtca gccgcactgt ggttcgtgaa gccatcatca tgctggaagt tgaaggctat
    29941 gtcgaagtgc gtaaaggttc aggtattcat gtggtttcca accagccgcg ccatcagcag
    30001 gcggctgaca ataatatgga attcgccaat tacggtccgt ttgagttgct ccaggctcgc
    30061 cagctcatcg aaagtaatat tgccgagttc gcggcaactc aggtaacgaa acaggacatc
    30121 atgaaattga tggccatcca ggagcaggcg cgcggcgaac aatgcttccg tgattccgag
    30181 tgggatttgc agttccatat tcaggtagcc ctggcgacgc agaactccgc cctggcggct
    30241 atcgttgaaa aaatgtggac ccagcgtagt cataacccgt actggaaaaa actgcacgaa
    30301 cacattgatg cccgtaccgt cgataactgg tgtgatgacc acgatcaaat cctcaaggcg
    30361 ctgattcgca aagatcctca tgctgctaag ctggcaatgt ggcagcacct ggaaaacacc
    30421 aagatcatgt tatttaacga aaccagcgac gatttcgagt tcaatgccga ccgctatctg
    30481 ttcgccgaaa acccggtggt gcatctcgat acagccacca gcggcagtaa atgaaattcc
    30541 tgtccgacag gcgctacagt gcgcctgttt gtctcgtcgg taagcgaaaa gtataaagtg
    30601 tcagcctgtg taaatcctct cgcctccctc ccctgcattc agcaaaatca gactccacgg
    30661 acatgtaatt ttgataacga acaacgttga cctttgttac aattagattc aatttgaatt
    30721 tatgcttttg aatgctttct tatctcacga tttaacaggg aatagttcag gctgtgttga
    30781 tgtatcaaac ccgcagaaca taccaagaca gcaataacat tgcggtagtg catcatttaa
    30841 aaccagcgtg gcgttaaccg attcaccagg aataatgaat ggaacttttg acccaattgc
    30901 tgcaagccct gtgggcgcag gattttgaaa ccctggccaa tccgtcgatg attggcatat
    30961 tgtattttgt cttgtttgtc attttgttcc ttgaaaacgg cttgcttccg gcggcctttt
    31021 taccgggcga cagtttactg gtactggtcg gcgtattgat tgcgaaaggc gcgatgggct
    31081 atccgcaaac gattctgctg ctgaccgtgg ccgccagcct cggttgctgg gtcagctata
    31141 ttcaggggcg atggctgggc aatacccgca ccgtacaaaa ctggctatct catttacccg
    31201 cgcattatca tcaacgcgca caccatcttt ttcataaaca cggtttatcg gcgctgttaa
    31261 ttggtcgctt tattgcgttt gtcagaacac tgctgccgac gattgccggg ttatcagggc
    31321 tgaataacgc gcgctttcag ttcttcaact ggatgagtgg tctgctgtgg gtgttgatcc
    31381 tgacaactct gggttacatg ctcggcaaaa cgccggtatt tttaaagtac gaggaccagc
    31441 tgatgtcatg cctgatgctg ctcccggtgg tgctgctggt gtttggcctg gcaggttctc
    31501 tggtcgtgtt atggaaaaag aaatatggaa atcgggggta agggatgcaa atacctcgca
    31561 tgtcacttcg ccagctagcc tggtccggcg ctgttttact tctggtcagc acactattgc
    31621 tggcctggtc cgcggttcgc cagcaagagt ctacgctggc gattcgtgcc gttcatcaag
    31681 ggacaacgat gccagacggt ttttcaatct ggcatcacct tgacgctcac ggcattcctt
    31741 tcaaaagtat cacccccaaa aacgacactc tgttaattac ctttgactcc agcgaccaga
    31801 gcgccgccgc aaaagcggtc ctcgacagaa cattgcccca tggctacatc attgcgcagc
    31861 aggacaataa cagtcaggct atgcagtggc tgacccggtt gcgggataac tctcatcgct
    31921 tcggataact tcctgttatc cgaaacagat cactcacttt ggtgatttca ccgtaactgt
    31981 ctatgattaa tgaggcggtg gttaacacga ccgcttcgtt gtctggacaa tcgttccttt
    32041 gtaataggtc cgaatcacaa tggaaggttc aagaatgaaa taccgcatcg ctttagctgt
    32101 ttctctcttt gctcttagtg ccggtagtta tgccactacc ctgtgtcagg aaaaggagca
    32161 aaatatcctt aaggagatca gctatgccga aaaacaccaa aaccagaatc gtattgacgg
    32221 tctgaataaa gccctgagtg aagtccgggc caactgttca gatagccaac tgcgtgccga
    32281 tcatcagaag aaaatcgcaa agcagaaaga tgaggtggcg gaacgccagc aagatttagc
    32341 cgaggcgaag caaaaaggcg atgcagataa gattgccaaa cgcgaacgga aactggcaga
    32401 agcgcaggaa gagctgaaaa agctggaagc gcgcgactac taactcacaa tagtcactac
    32461 ttactcacct ggagaaaact atgtcgaaag aacacactac ggaacatctg cgtgctgagt
    32521 tgaaatccct ttccgatacg ctggaagagg tgcttagctc atctggcgag aagtcaaaag
    32581 aagagttgag taagattcgt agcaaagcgg agcaggcact gaaacagagc cgttatcgcc
    32641 tgggtgaaac cggtgatgcc attgccaaac aaacccgtgt cgcggcggcg cgtgccgatg
    32701 agtatgtgcg tgaaaatccg tggacgggcg tgggcattgg cgcagcaatc ggtgtagtgc
    32761 tcggcgttct gctgtcgcgt cgttaattat ggcggacact catcacgcgc aagggcccgg
    32821 taaaagcgtt ctgggcatcg ggcagcgaat tgtttccatc atggttgaaa tggtggagac
    32881 acgtctgcgg ctggcggtgg tggagctgga agaggaaaaa gcgaatctct ttcaactttt
    32941 actgatgctg ggcctgacga tgcttttcgc tgcatttggt cttatgagcc tgatggtgct
    33001 aattatctgg gcggttgatc cgcaatatcg cctgaatgcg atgattgcca ccaccgtggt
    33061 gttgctgcta ctggcactga ttggcggcat ctggacgtta cgtaaatcgc gtaagtctac
    33121 gttgctgcgc cacacgcgcc atgagctggc aaacgatcgg cagctgctcg aggaggagtc
    33181 ccgtgagcag taaagtcgaa cgtgaacgac gtaaggcgca actgcttagc cagatccagc
    33241 aacaacggct ggatctttca gccagtcgtc gtgaatggct ggaggcaaca ggcgcttacg
    33301 atcgtcgctg gaatatgctg ttgagtctgc gctcctgggc gctggttggc agtagcgtga
    33361 tggctatctg gacgattcgc catcctaata tgctggtccg ctgggccaga cgcggttttg
    33421 gtgtatggag cgcctggcgt ctggtgaaaa caaccctcaa gcaacaacag cttcgcggtt
    33481 aacttcctct ctggccggag aaattccggc cttattcctc aaattttttg aaaatatttg
    33541 acagttttcc ttgctaacaa tcacgcttcg ccacgtttat gattctctcc atcgacagca
    33601 acgacgctaa tagcgcgccc ttgcacaaaa aaacaatcag cagcctgagc ggcgcagtgg
    33661 agagtatgat gaaaaaatta gaagatgttg gtgtactggt agcgcgtatt ttaatgccga
    33721 ttctgtttat taccgcaggc tggggaaaaa ttactggcta cgcgggtacc caacaatata
    33781 tggaagcaat gggcgtcccc ggctttatgc tgccgctggt gattctgctt gagtttggtg
    33841 gtggtctggc aatcctgttc ggtttcctga cacgtaccac agccctgttt actgcgggct
    33901 ttacgctgct gacggcattt ttatttcaca gcaattttgc agaaggcatg aactcgctga
    33961 tgtttatgaa aaacctgact atttctggcg gattcctgct gctggcaatt accggtccag
    34021 gcgcattcag catcgaccgc ctgctgaata aaaagtggta agcacgcgct atacttaacg
    34081 ataaaaaacg agggggaagc tcctcgtttt tgctattgga ggatagaaaa atgggtcaac
    34141 tgattgacgg cgtctggcat gacacctggt acgataccaa atccaccggc ggtaaatttc
    34201 aacgttcagc ttccgcattt cgtaactggc tcactgccga tggcgcgcca ggccccactg
    34261 gcaaaggcgg ttttgccgca gagaaagatc gttatcatct ctatgtttca ctcgcctgcc
    34321 cgtgggcaca ccgcacgctg atcatgcgta agctcaaagg actggaaccg tttatttccg
    34381 tttccgtagt gaacccgctg atgctggaaa acggctggac ctttgatgac agttttccgg
    34441 gagcaagcgg cgacacgctc tatcaacatg aatttctgta tcagctttat ctccacgccg
    34501 atccacacta cagcggacga gttactgttc ccgtgctgtg ggacaaaaaa aaccacacca
    34561 tcgtcagcaa cgaatcagca gaaatcatac gcatgttcaa taccgcgttt gatgcgctgg
    34621 gtgcgaaagc tggagattac tacccaccag ccctgcaacc gaaaattgac gaacttaacg
    34681 gctggattta cgacaccatt aacaatggcg tgtataaagc cggttttgcc accagccagc
    34741 aagcctacga cgaggcggtg gcgaaagtgt ttgaatcgct ggcgcgactg gaacagattt
    34801 taggtcagca ccgttacctg accggcaacc agctaaccga agccgatatt cgcctgtgga
    34861 ccacgctggt gcgttttgat ccagtgtatg tgacccactt caagtgtgat aagcaccgca
    34921 tcagcgatta cctgaatctg tatggcttcc tgcgcgatat ctaccagatg ccgggaattg
    34981 ccgaaacagt caatttcgat cacatccgta atcattactt ccgcagccat aagaccatca
    35041 accctacggg gattatttca attggtccgt ggcaggatct cgatgaaccg catggacgag
    35101 atgttcgctt cggttaaaaa aaggcacctt acgaggtgcc tctgataatt agctaagtac
    35161 gtgttgttca tgtcggatgc ggcgtaaacg ccttatcaaa cctacaaaac atttatattc
    35221 aatatgttgc aatgaccgcg caggccagat aagcgtagcg catctggcaa ttttgcgttt
    35281 gcccttatcc ttcacagcgc cttattaatt catcgcgata atatttactg ccatttcatt
    35341 cacgtctatt cttaatttgc tgctttaaaa acaagtgatt gagcaaaatt gaggcaaaaa
    35401 tggactggta tctgaaagta ctaaaaaatt atgtcggttt ccggggtcgt gcgcggcgca
    35461 aagagtactg gatgtttatt ctggtcaaca tcatctttac gttcgtgctg gggctactgg
    35521 ataaaatgtt aggctggcaa cgtgccggtg gcgaaggcat cctgacgaca atctacggta
    35581 ttctggtgtt tttaccatgg tgggcagttc agttccgccg cctgcacgac accgaccgct
    35641 cggcgtggtg ggcactgcta ttcttaatcc cgtttatcgg ctggcttatt atcatcgtct
    35701 ttaactgcca ggcgggtacg ccaggcgaaa accgctttgg accagatccg aaactggaac
    35761 aagaataata attaagcacc ttgtaaaagg tgcttaatcg attattttcc gctaaaaagt
    35821 ttgggaattt cccgcagaca ccaggatttc gcctccccca tactgtcacg acgccaggcc
    35881 ataataatat cgatctcgct ggtcgattcc gggctgacga cacgcaaccg cccttccgca
    35941 atatcttttt cgaccatcgg atacggcatc gtcgccacgc caagccccgc cagtaatgcc
    36001 tgacgtttat cttcaatcgt gctcaccgtt aagcgcggct gtttgtccag caactgtacg
    36061 gtcaacaccg ggcgctcacg agcggtatcc gccaccgcaa ttccacgata tttcacgcgc
    36121 gtcacttcag ataacggttc cggctcctga tgaatcgggt gatccggcgc ggcaacatag
    36181 acgttcatta aggtatagag tttgcgcgag ttgatctccg acgaggaacg aaaatgcata
    36241 tccggcgcga taacgatatc cgcccgcccc tgctccagcc gttcccacgc ccccgccagc
    36301 acttcggtga taattgccag ttgggtattg gcttttgccg ccagtttgtc gattaacggg
    36361 aaaaaggcag gtgtcggtac cagcgcctcg gtcacaatgg tgagatgcgt ttcccaaccg
    36421 cgcgcgaggg cttccgcatc ggtagtcagt ttatctgcgg cttccagcaa aacgcgccct
    36481 cgctccagca acatcctccc gacattggtg aatttggtgc gatggcccga gcggtcaaac
    36541 agcaccacat ccagctcttc ttccagtttt tgcatggtgt agctaagtgc ggaaggcacg
    36601 cgtcccagct catccgccgc cgccgcaaaa ctgccccggc gatcgatcgc atccataacc
    36661 cgtagtgctt ccagcgttaa tgccctttct ttggccattt cgttctcatt caggaaattt
    36721 gaacataccg ggcagaatat ctggctaaca atgtagcgtc cagcccctta ccataaaagg
    36781 aagtaaagag aggtcaagaa ttatgattac tacccgaact gccaggcagt gtggacaagc
    36841 agactacgga tggttgcagg cccggtatac tttttccttt ggacactact tcgacccgaa
    36901 attgttaggc tatgcctccc tgcgcgtgct taaccaggaa gtgctggccc caggtgccgc
    36961 ctttcagcca cgaacctatc ccaaagtcga tattttaaat gtgattctgg atggagaagc
    37021 tgagtatcgc gacagcgaag gcaatcatgt tcaggccagc actggtgagg tgttgctgct
    37081 ctctacccag ccgggtgtca gctatagcga acacaatctc agcaaagaca aaccgttaac
    37141 gcgaatgcag ctttggctgg atgcctgccc gcagcgagag aatccgctga ttcaaaagct
    37201 ggcgcttaat atgagcaagc agcaattaat cgcctcgcca gaaggtggga tgggaagcct
    37261 gcaattgcgc cagcaagtgt ggctgcacca tatcgtgctc gacaaaggcg aaagtgcgaa
    37321 tttccagttg catgggccac gcgcgtattt gcaatcgatt cacgggaaat ttcatgcgct
    37381 tacgcatcat gaagagaaag cggcgctaac ctgcggtgat ggggcgttta ttcgtgacga
    37441 ggctaacatt acgctggttg ccgattcccc actgcgcgct ttgctgatag atttgcctgt
    37501 ctagttgttt ttacagggag atgatgatga gtaaaaaatc ggccaaaaag cgccagccag
    37561 tgaagcccgt ggtggcgaaa gaacctgctc gtaccgccaa aaattttggc tatgaagaga
    37621 tgttgagcga actggaagcc atcgtcgcgg atgctgaaac gcgtttagcc gaggatgaag
    37681 ctaccgcgta aagacaggtt gccggatgcg gcgcaaacgc cttatccggc acaataccca
    37741 aaaccgggcg tttatctggc cttgctcgcc ataatctcga taatctgccg atccgtttgc
    37801 tgcatcgaat ggcttgctaa cgcacacagg ttggcaatcg actgctcaac atcatgcgcc
    37861 acaatccctt cattgccggt cacggcggta tcatccagcg ccattaacac cgctttccac
    37921 gcagccgaag cactggtcga aaccttcatc gcgcagctgt tcgatgcacc atcgcaaatc
    37981 atgccgctga catcgccgat catactgctg atcgccatcg agatggtttc ataacgccca
    38041 tccaccagcc atgccatccc ggcagcggcc cccattgctg cggtcgttgc ggcacacagc
    38101 gcagacaaac gcggtaactg gttatggatg taaatcgcgc tcaaatgcga aagcatcagc
    38161 gcacgcgcca gccgttcatc atccgctccg aagtgttcag ctaccaccac cacgggcatt
    38221 gttgcggtga tcccctggtt acccgagccg gagttactca ttgccggaag cgtagcgccg
    38281 cccatacgcg catcggatgc cgcgctggta cgaatcacaa tggatgaaga gagatctttc
    38341 gccagcaaac cgcgcgcgca ctgtttttcc agcgtcgcac caatatgcag cccccagtta
    38401 ccgctcaaac cttcctgcga taacgcacaa tttaacttcg cagaatcgag aataaagcgg
    38461 atcgccgcaa acgggacttc attgacgaac ttcaggatct cagccagcgt cgtcctggaa
    38521 agcaccgtca gcggcgactc ttgctcgccc tctgccacac acgcctgctg ggtaaacacc
    38581 acaccattgt gcgtctcaat atgcacaatg ttggtatgcc cgccgacgat ggtgacacac
    38641 gcccatttct caccgttcca gactttggcg cgtgagaaga ggatttcatc gcaaggttcc
    38701 tggatcttaa ctgagacttt ccccgccgcc agcagtgctt tggcatcgga aattgcctgc
    38761 gcagttgcgt ctttcagcac ttccagcccg gcgttagcat ttccacctaa cgctcccagc
    38821 gccgccgcaa ttggcagccc caccattcct gtaccgggaa cggtgacacc cagaccgttc
    38881 ttcatcagat ttggcgaaac ccaggcttct acacgttcaa ccggaccttc cagttcagcc
    38941 gcagcaaccg ccgccgccag cgccagtgaa atcggttcag tacaccccag cgccggtttt
    39001 acttcctcct gaacggcgag gatgtaacgc tgccataacg gatttaaagt cgaatcaaac
    39061 atattaaaaa ccttaaaatt tcaggtaaat caagaaaatg ccaggaacgg agaaacacaa
    39121 agcaacaaac cagtgacgat aatcaggtac aaagacatcc ctttgtattt gtgcaatgcc
    39181 ggtactttgt aaaccagcca cgccgggatc aggcacccta ccatgccgaa aatcgggcta
    39241 cagatagagg tgaagctcaa caccggggcg ttcagtacga tggcgctcca ggccagcaaa
    39301 atggcgaaaa tcatgatgcc gcgctgaacg agattttcgt taatcttctc ggcaggtatc
    39361 ttgcgacgca ggatgttcat tacgatccct tgcgttgctt cgcgaaagcc taaatagacg
    39421 ccaaagaatg cagtcattac agcaaagata ttgagaatga cgctgaccac tttcacccac
    39481 gctgcgccgt caccgctaat aaactgtgcg gcaatcgcca gcgcggaaat attctgctcg
    39541 taggctttta ccgcttcgtc atgtcccatc gccagcgtga acgacacggc gtagaaaaag
    39601 acggtgacaa acaaaatgcc aaacgcgata ttcattgccc gcaatgcttt atgccgcgcc
    39661 acttcaattg atttttcccg cgagcgataa gagatcacca tcgggcttaa cgtctggata
    39721 aatagaatcg acgtcagggt aaacggcagc gtaataatgg cgtttttcat cagcagcccc
    39781 agcggcggta gcgaaccgac gttatacaga tgccacattc ccaccatcga cacgcccagc
    39841 gccgcgacca ccagcagctt ggtcagcacc atgccggtcg aaattttgaa taacaatttc
    39901 tcgccgcgtg aggagatcgc caccagaatg caaatcagta ccagaccata aaagggactg
    39961 tctgacagca acccttccgt cacgccgaag gtatgcaggt aggaagcact atcgttggtg
    40021 atggcggtgg aatagacgaa catccaaatc accagcatta cgaaatagag tgcacctaac
    40081 aggatgcccc agtttttacc taaataaccg ctgatgacgc tcgggtaatc tttacattct
    40141 ggtgattctg ccagcgtatt aataaacaac cgctgaaaca gatacattgc cgggtaacca
    40201 atcaccgatg agagcaaaaa tacccacaat cccatcaaac cgacctgcac cgggagaaaa
    40261 acaatccccg cgccaatcgc catcccgata ctcataatca cccagccagt gtcagtactg
    40321 tcgaatttga tcgcttctcg ccactcgctc tcactcattc cagcacgacc cgccggggtc
    40381 gaagcgtctg caatgacgcc tttattcgat gcaatttcca taatttctcg ctcaatattt
    40441 tgtagggctt attatttttt tccgagccgc atcaaggcga tatgcggtct acgtttttgc
    40501 aggcgagtta ttagaataaa gaaatgatac gcgtgagatc agagaaaatc ttcacaatca
    40561 agacttttaa atagcaattg tgggtaataa ttttctatta aattgagtta atagaaataa
    40621 ttattctcta tttgcggcag atcacaataa aaaagggtgc acattttgtg cacccaagga
    40681 tgatagctga cagcaatgtc agccgcagac cactttaata gccagtcctc cgcgtgatgt
    40741 ttcgcggtat ttatcgttca tatctttgcc ggtttcatac atcgtctcga tcactttatc
    40801 gagtgaaaca cgcggtgccg aggtgcggcg catcgccatc cgcgcggcgt ttactgcttt
    40861 cacggcatta atggcattac gttcaatgca cgggatttgt acctgtccgg caaccggatc
    40921 gcaggtcagc ccaaggttat gctccatcgc gatttccgcc gcattgcata cctgcgccgg
    40981 actgccaccc agtagttcag ttaaccctgc cgctgccatt gaacaggcca cgccaatctc
    41041 cccctgacag ccgacttccg cgccagagat ggaggcgttc attttataca gcgcgccaat
    41101 tgcccccgct gccagaaaat agcgggcaat tgaccgctcg tttaccggac gacggaactt
    41161 atcgtaataa gccagcactg ccggaataat gccgcacgca ccgttagtcg gtgccgttac
    41221 cacgcgcccg ccagctgcgt tttcttcact aaccgccagc gcgtacatgt tgatccagtc
    41281 gatgacattc atcggatcgt tagagatgtt atcgctggaa accagttgac gacgcagcgc
    41341 tacggcacgg cgcggcacat tgagcggacc aggcagcacg ccttcagtat tcattccacg
    41401 ttcaataccg tcatgcatca cttgccagat acgggcaaac cctgcgtcaa tttccgcttt
    41461 actgcgcagc gccagctcgt tgtgcatcat caggccagat atagacaggc cgttgtaatc
    41521 acacattttc agcagttcac ctgctgagtg gaaatcgtaa ggtacgggtg tttcgacatc
    41581 gtgcgacagg ccgaagtgtt cttcttcgac aataaacccg ccgccgacag agtaataggt
    41641 tttgcttaat agctcttcct gtcccttcca ggcagtgatc cgcattccgt tctcatggcg
    41701 aggcaacatt tcgggatgga agataatgtt ctttgctaca ggaaaatcaa caatatgcgc
    41761 gccagaagcc accggcaacc gcccgctgcg cgtcactaac tctataaatg cagggatctc
    41821 atcaatgaca acatcctgcg gactgtttcc tgccagtccc atgatgatgg cgacatccgt
    41881 ggcatggcct ttgcccgtca gtgacaacga cccgtacaga tcgaccacaa tatggctcgt
    41941 cgcggttaat aagccgctac tttccagccg atcaataaaa ctttttccgg cattcattgg
    42001 ccccacagta tgcgaactgg agggaccaat cccaattttg aaaatatcga atgcactaat
    42061 catatccaca ccctcggatt gccgttcagt gaagtggagc ggaacgacct tgcgaccgtc
    42121 ccgctcacga ggctttacgc actacgtact gcgatggctt caatttccag cttcacatct
    42181 ttcggcaaac gcgcgacctg cacacagctc cgggtcgggt aggtcgcctg atgttcatcg
    42241 aagaactgct tatagacttc attgatggtg gcaaaatcat tcagatcggt gatgaacacg
    42301 gtcatcttga tgatatcgcc cacactcagc ccggcagcaa ccacgatcgc tttgacgttt
    42361 tcgaggctta aacgcgcctg atcttgcaca tcagccggga tctcaccggt ctgtgggcaa
    42421 accggaattt gcccggaggt gaagaccatg ctgcctaaat caacgccctg aacataaggg
    42481 ccgattgcgc ctggggcacg ttgcgtttcg ataatctttt tcatacatcc tccggcgtca
    42541 gagcgcctgg gtaaaggtac gtgaaataac atcctgttgc tgttcacggg tcagtgcgtt
    42601 gaagcgcacg gcgtagccag agacacggat tgtcaggtta ggatattttt ccgggtgctc
    42661 gatggcatcc agcagcattt cccgattcat tacgttgacg ttgaggtgtt gaccgccttc
    42721 gacatccgct tcgtggtgga aatacccatc cagcaggccg acaaggttgg ttttacgtac
    42781 tggatcttct ttgcccagcg ccgcagggac gatggagaag gtgtacgaga tcccatcttt
    42841 ggcgtaggtg aacggcagtt tcgccaccga cgtcaatgag gccacggcac ctttgcgatc
    42901 acgaccgtgc atcgggttag cgcccggcgc gaacggtgtt ccggcgcggc gaccgtccgg
    42961 cgtgttcccg gttttctgac cgtacaccac gttagaggtg atagtcagaa tcgattgggt
    43021 cggcacagca ttgcgatagg ttggcaaagc tttaattttc ttcataaagc gttcaaccag
    43081 atcgcaggca atgctgtcta cgcgctcgtc gttgttgcca tactgcggat attcgccgtc
    43141 aatctcgaaa tccaccgcca ggccgttttc atcacggatt ggtttcacac gagcatattt
    43201 gatggcagac agggagtccg ttgccaccga caggcccgcg atgccgcacg ccatggtgcg
    43261 atagacatca cgatcatgta gcgccatcag cgaagcttcg tagctgtact tgtcgtgcat
    43321 gtagtgaatg atattcagcg cgctgatgta ctgtaccgcc agccagtcca tgaagtggtc
    43381 gaggctatcc atcactttgt cgtagtccag cacgtcatcc atcaacggag ccgttttcgg
    43441 gccaacctga atcttcagct tctcgtccat tccgccgttg attgcgtaca gtagcgtttt
    43501 cgccaggtta gcgcgcgcac cgaagaactg catctgctta ccaatcacca ttgggctgac
    43561 gcaacaggca atcgcgtaat cgtcgctatt gaagtcagta cgcatcagat cgtcgttttc
    43621 atactgcaaa gaggatgtga cgatagacac ctgcgcggca tattttttga aggcaatcgg
    43681 caaggcttcc gaccacagaa tagtcaggtt aggttccggt gcaggcccca tagtgtgcag
    43741 ggtgtgtaaa tagcggaagg agtttttggt caccagggtt cgaccgtcca accccatccc
    43801 gccgatcact tccgtcgccc agatcgggtc gccggagaac agcgaatcaa attccggcgt
    43861 acgcaagaag cgcaccatac ggatcttcat gatgaagtgg tcgatcagtt cctgtgcctg
    43921 ctgctcattg agtacgcctg ctttaaagtc gcgctcaatg tagatatcga ggaacgatgc
    43981 cgtgcgcccc agcgacatcg cgccgccatt ttgcgatttc actgccgcca ggtaagcgaa
    44041 gtagagccac tgcacggctt cttgtgcatt ctgcgctgga cgagagatat caaagccata
    44101 tttcgccgcc atttcctgaa tctgtaacag cgcacggcga tgttctgcca gttcctcacg
    44161 cagacgaatg gtggcttcga gatcttcgcc tttttccaga cgggactgga gatcggcaaa
    44221 ctgcagttcg cgttcacgta ccagataact gatgccgtac agtgccacac ggcgatagtc
    44281 accgatgata cgcccacgac cataaccatc tggtaaaccg gtcagtacac cagatttacg
    44341 gcagcgcagc atatccggtg agtaagtatc aaatacgccc tgattatggg ttttacgcag
    44401 atcggtaaag atgtattcaa attcgctatc catttcacga ccatacgcgt ggaatgaact
    44461 tctaatcata ttaatgccgc cgaacggatg cagcgcacgc tttaatggcg catccgtttg
    44521 cagacccact attttttcca gcggctggtt aatatatccc gcgtcatgag cggtaattgt
    44581 ggtggcaata ttggtatcga aatccaatgg cgcgtgagtc gcattttcga tacggatgcc
    44641 ttccatgact ttttcccaca attccgtggt ggcaggcgtc gcttcggcga gaaaagattc
    44701 atcgccttca taaggtgtat agttatgttg aataaaatcg cggacattaa tttcgttttt
    44761 ccagtccgta cctttaaagc caagccatgc gtcggcgtac agcttatcgc tggtatcaat
    44821 atctaccttc atgaaaaata atctctctac aatacttcaa ctaaattatg caaattctgc
    44881 gggcgcgttc actttgccta aatgaatggc atccaaagca atcatttttt cttcgttagt
    44941 cggaattacg gcgcaaatga cacgcgcatt ttcactggaa acaattcgct ctccaaaaga
    45001 gttagagcga ttattcattt ctgtatcaat ttctacgcct aatacagcta aatgttccat
    45061 gaccagacga cgaattaaac ttgaattctc tcctattccg ccggtgaata taattccatc
    45121 caggcgatgt aatgaagcag cgtgtccggc aatatgacgg gcaattcggt gaacaaaggt
    45181 tttaattgcc agttgcgcgc gttcgtgacc ttcatgccag gctttttcca gaacacgtaa
    45241 atccgaagaa aggccggaaa tacctaataa tcccgactct ttattcacta cgcgttccag
    45301 gtcccccagg ctctggttgg tttggctggc gacccaggac atcgccccga agtcgacatc
    45361 accactgcgg gtacccatca tcaagccttc cagcggcgtc attcccattg aagtgtcaac
    45421 actctgaccg ttgcgaaccg cgcagattga tgcgccattg ccaagatgcg ccacaaccag
    45481 gccggagtca tcttccgcca gattcagcag cgaatgtgcg cgctgggaaa catagcggtg
    45541 cgacgtgccg tggaaaccat aacggcgtac acctaactct tcataatatt tccacggcag
    45601 gccgtataaa taagcttccg gagccatcgt ctggtggaaa ctggtatcaa ataccgccac
    45661 ctgagttacg cccgggaata attgctgcgc cgattcaata ccacttaaat tggcgtaatt
    45721 atgcaggggt gccagtggag aaacgcgacg gatattatca atgacttcat cggtaataat
    45781 ggcggactcg gtaaaaatac tgccgccgtg agcgatgcgg tggccaatta aagccacact
    45841 gtcatttaaa ttccgttttt ccagttcaaa tgcaattgcc ttcaatgcac cttcgtagct
    45901 gtggtgagcc agcggtgctg gctctccccc atttacggat aagaatgcat tttccgagtt
    45961 aataccgtcg gcaatacctg acattaatac ttcacagtcg ctggcattga gtacggaaaa
    46021 cttaatcgaa gacgaaccac agttaataac caaaacaacc ggaaattcat tcatctcttt
    46081 tctcatcctg agttacggat taaaacagtt tgtatacgat gttcaggatg gtcagcagac
    46141 caatcacggt aacaaacacg ttatccagac gaccacggta tttcgccaga gacggcgctt
    46201 tacggatggc atacatcggc aacaggcaca gcagtgatgc gataatcggt gcgcccatgg
    46261 cttcaatcaa gtcgaggatg ttcgggttgg cgtaggcaac aacccaggtg gagcccataa
    46321 tgaaaatcat gctgatagta ttcagtttac ccagcgacac tttggttttg tcacctttat
    46381 aaccgaactt cagaatcaaa ccattcagcc cttccagcgt ccccagatag tgaccgaaga
    46441 aagatttgaa aatagccacg agtgcgatga tggaagcggc atattccagt gtaatcgcaa
    46501 acgttgtttt ggtaccggtc atggacgcaa agtggttagc cagataagaa agcactggaa
    46561 tattctgcgc tttcgcttca gccatattcg ccggagacag cgtaaacagg cagctaaagg
    46621 caaagaacat caccactgca accatcagca tactggcgcg agagatgatt tgtgaacatt
    46681 tacgctcggt gaagtcgcga ccaaaatctt tctcgtactc ttcacgttta gaaaccacga
    46741 aggaagagac gattggcgag aagttaaagg agaaaaccat gatggaaatc cccagccaga
    46801 cggtgatcag gataccgtca tgaccagtta acgacagcga accgaggtca acctggtcga
    46861 taactgcaga gttccagtaa gggatcagcg aaagagaaat cagcaccagg ctggcgataa
    46921 acggccatac cagatagctc atcactttaa ccatcagatc cttaccaaac cagatgacga
    46981 aagccatcag cagcaacagg aacagcgcca caaagccgcg attcagcggt gcaaagccga
    47041 gctggttttc ccagaacgtc ataaaggtgt tggtaatggt aacgccataa atccacaaca
    47101 gtgggcaaat cgcgaagaag tacaggaacg tgataaccac gccgccagtt ttaccaaaat
    47161 gctcttccac cgtttccgta atgttgccgg aagggttaga gccagaaaga cacagacgcg
    47221 ccagtgcccg gtggcaataa aacgcgatgg ggtatgctaa taccaacatc agaagaatcg
    47281 ggatcagtcc gccaaaacct gcgcggatag ggaagaacag caccccggcg ccgattgccg
    47341 taccaaacaa gcctaacgtc catgtggtat ctgatttacg ccaggacgat tgttttgtct
    47401 ggctggatac aatgctatct gaagtactca tatcctatcc tcaacgaatt aattaagcgt
    47461 caacgaaacc ggtgatttga gagacgcgag aaagatcgat attgccgccg gaaataatac
    47521 tgacggtttt tctgttttga atatattggt ctaatttacc gcttaataat gcagcacatg
    47581 ccagagcgcc tgcgccttcg gtgacgactt tattgcgctg aattaaggca atcatactgt
    47641 ttctgatttc gtcttcgctg accagcacga tgtcatcgac taattcacga acgatttcgt
    47701 aagttaaatt acccgggcgg gagacatcac aaccatccgc cagggtgccg gtagttcggt
    47761 gcgtagttat ttctccggag tggaaagaag ccgccatgcc gtgaacgttt tcagactgta
    47821 cgccaataac acggatggtc gggttgatag atttaattgc caccgcaata ccagcaatta
    47881 aaccgccgcc gccaattggc acaatcacgt tatcgacatc atagagatct tccataattt
    47941 ccagaccaat cgttccctgg ccagcaatca ctttcggatc atcgtaaggt gggataaaaa
    48001 tacggccttc catttcgaca atttcgctca ctttagcaat agtgtcgttg aagttatcac
    48061 catgcaaaac gacttctgcg gagtagtcgc acgttgccgc gactttggat ttcggcgcac
    48121 cttttggcat caccacttta ccgtcaatac ccagcatcgc gcaggagagc gaaacccctt
    48181 gcgcatggtt gcccgcagag caggccacta ctcctttacg tttttccgca tcagtcagtg
    48241 aacttaattt attaaatgcg ccacgaattt taaatgaacc agtacgctgc atgttttcga
    48301 atttgaggaa tatttcacct ttgcaacgtt cactaaaata gttggagcga ggcatacctg
    48361 ttttataaat tcgcccagcc agtcgttgtt ttgcttcaat gatgtcatca atagcaaccg
    48421 ggagatcgta tgtaatatgc attataaaac ctcttagcct gaattaaata tgtaggtaac
    48481 cgtaaccgac acctgcaaaa cagacaggtg gattatttat gtttaaatta aaacaaataa
    48541 ctaatcaact tcaattaatt gccttcgtct acacccatta taagatgaat actctttggc
    48601 taattccacc aaaaccgatg ctgctttttt aatacgataa tttttcgacc ataccgcggc
    48661 atattgtgcc acaggtaatg tttcttcaac cggaatagta ataaattgat tagaaccaaa
    48721 aggtgatgtc atatcacaag gaattacagt taagaaatca gcattgagaa caagattata
    48781 aattgtcacg actgagtcgg ttttaacgat gttttcaata ctgatgccat ttctttgtaa
    48841 cgtagtaagt agttcgctgt agtaccccat attggtttgt ggcaacaccc actgttcgtt
    48901 cttcaacgac tccagcgtgg cggtgccggt gcatgttcgg gacttactgg ctaccagcac
    48961 aaactcggac tcgaacagcg gctcaacatg taaatcctga agcttcattt ctgcgcttaa
    49021 cgtaccaatc gcaaaatcca gtcgaccgtc gcggattgcc ggtaagaacg aagacagttg
    49081 cgcttcatac atagaaacct gcgctttcgg gaacacctct ttgaacttgt tgatcatccc
    49141 tgacataaaa gtaaaaccaa tcaatgaagg aaaaccaaat gagacttcca ccaccgcctc
    49201 agaagacata ccgcttatct cattaaccat atttttcatt tcacgggtaa tggattcgga
    49261 acgggagagt aacaattgac cggcaggtgt taatgttaca ccggtatttt tccgcaccac
    49321 taactccacc ccaaaataat cttcaatatc gttaatgatt ttactgacgg ccggttgagt
    49381 taaccctaac tcttttgcag ccgagccgat agaaccactt ctaataactt cctgaaagac
    49441 taccaggtgc tgcgttttcg gaagaagaat agtgctcata acgacctacg ttaattacct
    49501 cattgacggt atgaagtgta tcaaaatgaa atgaacagga tatgtgcgac cactcacaaa
    49561 ttaactttca atactttcca tagcatcgtt accccaatta aataccttta agtatcaaca
    49621 agttaaagta tgaaaatcag cataaaccct gatttttgtc aaaaaaaatg acggggataa
    49681 ccatatttta tggcgataac atcattcttt atgtggttaa tacaaaaaag gggctgagac
    49741 tatatttcaa gcaattaccg gataattacc aggcaaaatt tgagttaaat ttaatgagta
    49801 agttctcaaa ttttattata aacaaaccat tttcagcgat aaataatgcg gcacgtcaca
    49861 ttttttaacg ctatttgttg gagaacaaac atttatttta tcaatatttt aaaatttcga
    49921 atacaggtat tgatcatctc gaacaattga ttaacgtcaa cgttttctct tctgacagga
    49981 tgtcattttg tgaatgcaat cgttttccat aaattcttct cccatcatag gcaacgaaga
    50041 gcattttgtg ttgaggatca caaaacgaat aattgctggt cgccgcgata aggtcagacg
    50101 aagacaacaa gggggattct cactgtgctt tacatcagcg taggccatag aatgttgctt
    50161 cgttgactgt aatgccgggt aaatgagcgt tttttgatag tgcgaaagaa ccgtgcaagg
    50221 agaataagca tctattattg tttgtcatga tgacaaggca ggcatcaatg aaaggcttct
    50281 caattgcaca tattcttcac ccttcaatcc cgtcaatgca cgcagtggtt aacaatcaca
    50341 atagaaatat tgattattgg acggtaaaaa tttaggttat aacgcccaaa ttattcaacg
    50401 atcggacaat acctgcgaac ttacaattaa tgagagaaat atacgtataa ttacgacctt
    50461 ttaaatgcaa tatagactta ataacttctt acaaagtctg ttattaatac cgtagatatt
    50521 catcttaccc tgggcacaca aagcaaaaac ccgccgaagc gggtttttac gtaaatcagg
    50581 tgaaactgac cgataagccg ggttctgtcg tggacagtca ttcatctagg ccagcaatcg
    50641 ctcactggct caagcagcct acccgggttc agtacgggcc gtaccttatg aacccctatt
    50701 tggccttgct ccgggtggag tttaccgtgc cacggactgt taccagccgc gcggtgcgct
    50761 cttaccgcac cctttcaccc ttacctgatc ccgcttacgc gggccatcgg cggtttgctc
    50821 tctgttgcac tggtcgtggg tttccccccc aggcgttacc tggcaccctg ccctatggag
    50881 cccggacttt cctcccctcc gcccgtctcc cccgaagagg acgacgacga agcggcgact
    50941 gtctggtcag cttcggcgcg cagtatagag ggtttgcgcg cccttgtcac cccgcgttgc
    51001 gcattccaat cgccagtgtc gcggcgatat tacgtgaagc acggcagata ttgtcataag
    51061 ctccgcggag tgcttcgtct aacgtaccaa tgctggtcag tacgctgaag accgcatcaa
    51121 tgccatgctg atgtacaacg ccaacatcat tggtcaggct acccgcaatg ccaatcaccg
    51181 gtttatggta cttcttcgcc acgtttgcga caccaatcgg taccttgccg tgaatactct
    51241 ggctatcaat acgcccttca ccagtgatca ccagcgtaca atcgtgaata tgttcctcca
    51301 gattcagcgc cgtagtgacg atttcaatac cacttttcag ttctgcacca agaaacgcca
    51361 tcagcgccgc gcccatacct cccgcagctc ctgcgccggg gacatctttc acatcaacat
    51421 gcagcgcttt tttaataaca tcggcatagt gagagaggtt attgtccagc tcgacaatca
    51481 tcgcctcact ggctcccttt tgtgggccaa agatgcgcga agcaccactg tcgccgacta
    51541 acgggttggt gacatcacaa gcgacgcgga tgacacaatc ttttaagcgc ggatcgagac
    51601 cggaaatatc aatatcattc agagtattaa gactaccgcc gccaaaacca atttcattgc
    51661 cgttggcatc gcataatttc gcccccagcg cctgtaccat gcctgcgccg ccgtcgttgg
    51721 tggcgctgcc gccaatgccg ataataatgt ttgtcgcacc gctctccagc gcctgcagga
    51781 ttaactcacc agtaccgcgt gaagtggtca cgagtggatc gcgtttttcc gcaggtacca
    51841 gttccagccc actggccgcc gccatttcga taaacgcggt tttgccatcg ccggagatcc
    51901 cccaactggc attcactttc tcgcccagcg gccctgtaac ccaggcgtga cgttcagccc
    51961 cctgggtggc tgcaatcatc gcttccaccg ttccttcacc accgtcggca accggaacag
    52021 aaacatactg tgcatcagga aaaatttccc gaaatccttt ttctatcgcc tgcgcaacct
    52081 cgctggcaga taaactttct ttataagagt ctggggcgat tacgattttc atacctatgc
    52141 ctgttaccac atgacgccgg agggcgtttc tcttattcgg cctggattcc aggcccggat
    52201 tgcaatacgc catccgggca cgacgtcatt agcgagtaac ttcgactttc gccagttttt
    52261 cgtagtagca cgccagggcg ctatgatccg ccgttcctaa accatctgca cgcagtgcct
    52321 gcatcatctc cataaccgca gcagtgagcg gcagttgcgc gccgacgccg tgagaagtat
    52381 ccagcgcatt cgccagatcc ttaatatgca gatcaatacg gaagcccggt ttgaagttgc
    52441 ggtccatcac catcggcgct ttggcatcca gcacggtact gcccgccagc ccaccgcgaa
    52501 ttgcctgata caccagatcc gggttaacgc ccgctttggt tgccagcgtt aacgcttctg
    52561 acatcgcggc aatattcagc gccacaatga cctgatttgc cagtttggtg acgttacctg
    52621 caccgatttc cccggtatgc accacggaac ccgccatcgc tttcatcaaa tcatagtatt
    52681 tgtcgaaaat agccttgtcg ccgcccacca tcactgacag cgtaccgtcg atggctttcg
    52741 gttcaccgcc gctcaccgga gcatccagca tatcaatgcc tttcgctttc agcgcttcgc
    52801 tgatttcacg gcttgccagc ggtgcgatag aactcatatc gatcaatacc gtacctggct
    52861 tcgcgccttc aataatgcca ttctcaccca gcgccacctc tttcacatga ggggagtttg
    52921 gcagcatggt tatgatgacg tcgcactgtt cagcgatcgc tttagccgta gcggctgttt
    52981 ctgcaccggc agcaatcacg tcagcaatag cttctgggtt acggtcagca accaccagcg
    53041 agtaacctgc tttcagaagg tttttactca ttggtttacc cataatcccc aggccaataa
    53101 aaccaacttt catagtcata tcaatcatct ctcttgttgc ggtggtggtt attttttaaa
    53161 ggtatcagcc agtttctgag tggcagagcg gaagacgccg agatcgctgc cgacggccac
    53221 aaacgtcgcg ccccattcca gataacgacg cgcatcagcc tcgaccggtg cgaggatgcc
    53281 gctgggtttg ccgtgcgcgc tggcacggtt aaaaatgtgc tgaattgctt tttgtacatc
    53341 cgggtgcgag gcattgccga gatggcctaa tgccgcagcc agatcgctgg ggccgacgaa
    53401 gatgccgtct acaccttcgg tagcggcaat agcatcgacg ttatctacgc cctgctgact
    53461 ttctatctgg accagaatag tgatgttctt gttcgactga gcgaaataat ccgccacggt
    53521 gccaaacata ttggcgcggt gagaaacgga gacgccgcga atgccttccg gtgggtaacg
    53581 agttgatgcc accgcctgct ctgcttcctc ttttgtttct acaaaaggaa tcaggaagtt
    53641 atagaaaccg atatccagaa gacgcttaat aattaccggc tcgttggtcg gcactcgcac
    53701 tactggcgcg ctggcgctgc ctttcagggc cattaactgc ggaataaacg tggagatatc
    53761 gtttggcgca tgttcaccat ccagcaccag ccagtcaaac ccagccaaac caagaacttc
    53821 agtgctaatc gggttagaga gagctgacca gcaaccaatt tgtacctgtt tcgcagccag
    53881 tgcggctttg aatttattcg ggaaaacatc gttattcatc gcttatacct ttgcttattt
    53941 ctgcaattcc atacgtttaa tgtcgccgac tacgaagagg tagcagacca tcgccatcag
    54001 cgctgaacat cccacgaaaa ccagtgctgc attgaaggag tgcagttcac ttaccagata
    54061 gccaatcacc agtggagtga caatggaggc aacattgcca aagacgttaa agacgccgcc
    54121 gcagaggcca acaatctctt tcggcgcggt gtcagaaatc actggccagc ccagcgcacc
    54181 aaatcctttg ccaaagaaag ccagcgccat cagcatgacc accagcgtgg tgttgttggt
    54241 gtagttacat aagatgatgg tggaagccag caacattccc agcacaattg gtagcttacg
    54301 tgccagggtc agggacaaac cgcgtttgat cagataatcc gagaagacac ctcccagcac
    54361 gccgcccgca aagccacaca gtgccggaat cgaggcgacc agacccactt tcagaatcga
    54421 catgcctttt tcctgcacca gataaattgg gaaccaggtg aggaagaacc aggtgatggt
    54481 gttgataaaa tattgtccga aaaatacgcc cagcatcatg cggttagaga gcaattgctt
    54541 gatgtaatgc agtttgggtc cgcttgctgc cgcactaccc ggctttttgt ggtccatatc
    54601 gaccaccgcg ccattttccg agataaactt cagctcttct gcagacatac gtgggtgatc
    54661 tgtcgggtta tgaatcaact tgatccacag cgccgtcaac acaaaaccaa tcacccccat
    54721 aacggtaaag acatgctccc agccccaggc gaaagtcagc cagccaagca gcggcgaaaa
    54781 gagcgccaac gagaaatatt gcgccgagtt aaagatggcg gaggcagtac cacgttcttt
    54841 cgtcgggaac caggcggcga caatacgggc gttcgccggg aatgatggcg cttccgagaa
    54901 ccccagcata aatcgcataa agaacatgga gatcccagcc caggccagcg gaaacatatc
    54961 gacaaagcct tgcaggaagg tgaacagcga ccagaaaaag aggctgtaag tgtaaacttt
    55021 cttcgagcca aacttatcaa gcagccagcc gccgggaatt tgcatcagca agtaggccca
    55081 gccaaaagca gagaagatgt aacccatcga aaccgcactt aactgcaact cttttgccac
    55141 ttcggtacca gcaatagaca gcgttgcacg atcggcgtag ttaacggcgg taacaataaa
    55201 aataatcagt aatattaaat agcgggtatg cacgcctttc ttttttacgt caacggtgtc
    55261 cagaatcatt ttatttacct cgggtactta tgctgatttt tattattatg gggaaggtg
//
LOCUS       DAHHTG010000013        55120 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.013, whole genome shotgun sequence.
ACCESSION   DAHHTG010000013 DAHHTG010000000
VERSION     DAHHTG010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 55120)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 55120)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..55120
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.013"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            161..1381
                     /gene="arcA"
                     /locus_tag="NBI74_000798"
     CDS             161..1381
                     /gene="arcA"
                     /locus_tag="NBI74_000798"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HCD1931171.1"
                     /translation="MEKHYVGSEIGQLRSVMLHRPNLSLKRLTPSNCQELLFDDVLSV
                     ERAGEEHDIFANTLRQQGIEVLLLTDLLTQTLDIPEAKSWLLETQISDYRLGPTFATD
                     VRTWLAEMSHRDLARHLSGGLTYSEIPASIKNMVVDTHDINDFIMKPLPNHLFTRDTS
                     CWIYNGVSINPMAKPARQRETNNLRAIYRWHPQFAGGEFIKYFGDENINYDHATLEGG
                     DVLVIGRGAVLIGMSERTTPQGVEFLAQALFKHRQAERVIAVELPKHRSCMHLDTVMT
                     HIDIDTFSVYPEVVRPDDNCWTLTPDGHGGLKRTQESTLLHAIEKALGIDQVRLITTG
                     GDAFEAEREQWNDANNVLTLRPGVVVGYERNIWTNEKYDKAGITVLPIPGDELGRGRG
                     GARCMSCPLHRDGI"
     gene            1392..2324
                     /gene="arcC"
                     /locus_tag="NBI74_000799"
     CDS             1392..2324
                     /gene="arcC"
                     /locus_tag="NBI74_000799"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006686402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HCD1931172.1"
                     /translation="MENKPTLVIALGGNALLKRGEPLEAEIQRKNIDLAAKTIAQLTQ
                     HWRVVLVHGNGPQVGLLALQNSAYAHVAPYPLDILGAESQGMIGYMLQQALKNQLPQR
                     EISVLLTQVEVDANDPAFSNPTKYIGPIYDHAQTQVLQAEKGWVFKADGHSFRRVVPS
                     PQPKRIVERDAIQTLIAHDHLVICNGGGGVPVVEKADGYHGIEAVIDKDLSAALLASQ
                     IHADALLILTDADAVYLDWGKPTQRPLAQVTPELLNEMQFDAGSMGPKVTACAKFVSQ
                     CRGIAGIGSLADGPEILAGDKGTLIRLDTPHNHA"
     gene            2348..3352
                     /gene="argF"
                     /locus_tag="NBI74_000800"
     CDS             2348..3352
                     /gene="argF"
                     /locus_tag="NBI74_000800"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HCD1931173.1"
                     /translation="MATSLKNRNFLKLLDYTPAEIQYLIDLAINLKAAKKSGNEKQTL
                     VGKNIALIFEKSSTRTRCAFEVAAFDQGAQVTYIGPSGSQIGHKESMKDTARVLGRMY
                     DGIEYRGYGQNIVEELGEFAGVPVWNGLTNEFHPTQILADLMTMLEHAPGKTLPELSF
                     AYLGDARNNMGNSLMVGAAKMGMDIRLVAPKSFWPDETLVTQCREIASVTGARITLTE
                     DVEEGVYDVDFLYTDVWVSMGEPKEAWAERVSLMAPYQINQQVITATRNPEVKFMHCL
                     PAFHNEHTTVGREIEMAYGLKGLEVTEEVFESAHSIVFDEAENRMHTIKAVMVATLGD
                     "
     gene            3409..4812
                     /locus_tag="NBI74_000801"
     CDS             3409..4812
                     /locus_tag="NBI74_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HCD1931174.1"
                     /translation="MGKFKFPTAYTILFVLIALVAAMTWIVPAGKYQMVTNAALGKEV
                     PVAGTYAPVEAQPQGITAVLLAPIDGLYNHQTYTAGAIDVALFVLIIGGFLGVVNKTG
                     AIDAGIERVTTRLNGREEWMIPILMALFAAGGTIYGMAEESLPFYTLLVPVMMAARFD
                     PLVAAATVLLGSGIGTLGSTINPFATVIAANAAGIPFTQGILLRVILLLVGYVICVYW
                     VMRYARKVRNSPESSIVADKMAENQAHFLGNRSETMLEFTPTRKAILILFAASFAFMI
                     YGVAVLGWWMAEISAVFLAAAVIVGVIARMGEETFTSTFIDGARDLLGVALIIGIARG
                     IVVVMDNGMITHTILHSAENLVSGLSTTVFINVTYWLEVLLSFLVPSSSGLAVLTMPI
                     MAPLADFAHVQRDLVVTAYQSASGVVNLITPTSAVVMGGLAIARVPWVRYLRWVAPLL
                     LILTMLNMIVLSIAAMI"
     gene            4928..5416
                     /locus_tag="NBI74_000802"
     CDS             4928..5416
                     /locus_tag="NBI74_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HCD1931175.1"
                     /translation="MKEYDDYSAKEKKQLAVCQRLITEKSYLSQEEIRRDLQNHGFDS
                     ISQSTVSRLLKLLGVIKIRNTKGQKIYSVNPQLLPTPDAGRSVAEMVLSVEHNGEFIL
                     IHTVAGYGRAVARILDFHALPEILGVIAGSNIVWVAPRVVKRTALVHKQINYLLKLNI
                     YS"
     gene            5506..5640
                     /gene="pyrL"
                     /locus_tag="NBI74_000803"
     CDS             5506..5640
                     /gene="pyrL"
                     /locus_tag="NBI74_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyr operon leader peptide"
                     /protein_id="HCD1931176.1"
                     /translation="MVQCVRHFVLPRLKKDAGLPFFFPLITHSQPLNRGAFFCPGVRR
                     "
     gene            5644..6579
                     /gene="pyrB"
                     /locus_tag="NBI74_000804"
     CDS             5644..6579
                     /gene="pyrB"
                     /locus_tag="NBI74_000804"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HCD1931177.1"
                     /translation="MANPLYQKHIISINDLSRDDLNLVLATAAKLKANPQPELLKHKV
                     IASCFFEASTRTRLSFETSMHRLGASVVGFSDSANTSLGKKGETLADTISVISTYVDA
                     IVMRHPQEGAARLATEFSGNVPVLNAGDGSNQHPTQTLLDLFTIQETQGRLDNLHVAM
                     VGDLKYGRTVHSLTQALAKFDGNRFYFIAPDALAMPQYILDMLDEKGIAWSLHSSIEE
                     VMAEVDILYMTRVQKERLDPSEYANVKAQFVLRASDLHNAKANMKVLHPLPRVDEIAT
                     DVDKTPHAWYFQQAGNGIFARQALLALVLNRDLVL"
     gene            6592..7053
                     /gene="pyrI"
                     /locus_tag="NBI74_000805"
     CDS             6592..7053
                     /gene="pyrI"
                     /locus_tag="NBI74_000805"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase regulatory
                     subunit"
                     /protein_id="HCD1931178.1"
                     /translation="MTHDNKLQVEAIKRGTVIDHIPAQIGFKLLSLFKLTETDQRITI
                     GLNLPSGEMGRKDLIKIENTFLSEDQVDQLALYAPQATVNRIDNYEVVGKSRPSLPER
                     IDNVLVCPNSNCISHAEPVSSSFAVRKRANDIALKCKYCEKEFSHNVVLAN"
     gene            7126..7512
                     /gene="ridA"
                     /locus_tag="NBI74_000806"
     CDS             7126..7512
                     /gene="ridA"
                     /locus_tag="NBI74_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminobutanoate/2-iminopropanoate deaminase"
                     /protein_id="HCD1931179.1"
                     /translation="MSKTIATENAPAAIGPYVQGVDLGNMIITSGQIPVNPKTGEVPA
                     DVAAQARQSLDNVKAIVEAAGLKVGDIVKTTVFVKDLNDFATVNATYEAFFTEHNATF
                     PARSCVEVARLPKDVKIEIEAIAVRR"
     gene            complement(7718..10414)
                     /gene="mgtA"
                     /locus_tag="NBI74_000807"
     CDS             complement(7718..10414)
                     /gene="mgtA"
                     /locus_tag="NBI74_000807"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HCD1931180.1"
                     /translation="MFKEIFTRLIRHLPSRLVHRDPLPGAQQTVNAAVPPSLSAHCLK
                     MAVMPEEELWKTFDTHPEGLNLAEVESAREQHGENKLPAQQPSPWWVHLWVCYRNPFN
                     ILLTILGAISYATEDLFAAGVIALMVAISTLLNFIQEARSTKAADALKAMVSNTATVL
                     RVINDKGENGWLEIPIDQLVPGDIIKLAAGDMIPADLRILQARDLFVAQASLTGESLP
                     VEKAATTRQPEHSNPLECDTLCFMGTTVVSGTAQAMVIATGANTWFGQLAGRVSEQES
                     EPNAFQQGISRVSMLLIRFMLVMAPVVLLINGYTKGDWWEAALFALSVAVGLTPEMLP
                     MIVTSTLARGAVKLSKQKVIVKHLDAIQNFGAMDILCTDKTGTLTQDKIVLENHTDIS
                     GKTSERVLHSAWLNSHYQTGLKNLLDTAVLEGTDEESARSLASRWQKIDEIPFDFERR
                     RMSVVVAENTEHHQLVYKGALQEILNVCSQVRHNGEIVPLDDTMLRKIKRVTDTLNRQ
                     GLRVVAVATKYLPAREGDYQRADESDLILEGYIAFLDPPKETTAPALKALKASGITVK
                     ILTGDSELVAAKVCHEVGLDAGEVVIGSDIETLSDDELANLAQRTTLFARLTPMHKER
                     IVTLLKREGHVVGFMGDGINDAPALRAADIGISVDGAVDIAREAADIILLEKSLMVLE
                     EGVIEGRRTFANMLKYIKMTASSNFGNVFSVLVASAFLPFLPMLPLHLLIQNLLYDVS
                     QVAIPFDNVDDEQIQKPQRWNPADLGRFMVFFGPISSIFDILTFCLMWWVFHANTPET
                     QTLFQSGWFVVGLLSQTLIVHMIRTRRVPFIQSCASWPLMIMTVIVMIVGIALPFSPL
                     ASYLQLQALPLSYFPWLVAILAGYMTLTQLVKGFYSRRYGWQ"
     gene            complement(10555..10608)
                     /gene="mgtL"
                     /locus_tag="NBI74_000808"
     CDS             complement(10555..10608)
                     /gene="mgtL"
                     /locus_tag="NBI74_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_003933616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mgtA regulatory leader peptide MgtL"
                     /protein_id="HCD1931181.1"
                     /translation="MEPDPTPLPRRRLKLFR"
     gene            10793..11740
                     /gene="treR"
                     /locus_tag="NBI74_000809"
     CDS             10793..11740
                     /gene="treR"
                     /locus_tag="NBI74_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator TreR"
                     /protein_id="HCD1931182.1"
                     /translation="MQNRLTIKDIARLSGVGKSTVSRVLNNESGVSQRTRERVEAVMN
                     QHGFSPSRSARAMRGQSDKVVAIIVTRLDSLSENLAVQTMLPAFYEQGYDPIMMESQF
                     SPQLVAEHLGVLKRRNIDGVVLFGFTGITEEMLAHWQSSLVLLARDAKGFASVCYDDE
                     GAIKILMQRLYDQGHRNISYLGVPHSDVTTGKRRHEAYLAFCKTHKLHPVAALPGLAM
                     KQGYENVAKVITPETTALLCATDTLALGASKYLQEQRIDTLQLASVGNTPLMKFLHPE
                     IVTVDPGYAEAGRQAACQLIAQVTGRSEPQQIIIPATLS"
     gene            11859..13280
                     /gene="treB"
                     /locus_tag="NBI74_000810"
     CDS             11859..13280
                     /gene="treB"
                     /locus_tag="NBI74_000810"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS trehalose transporter subunit IIBC"
                     /protein_id="HCD1931183.1"
                     /translation="MMSKINQTDIDRLIELVGGRGNIATVSHCITRLRFVLSQPANAR
                     PKEIEQLPMVKGCFTNAGQFQVVIGTNVGDYYQALIASTGQAQVDKEQVKKAARQNMK
                     WHEQLISHFAEIFFPLLPALISGGLILGFRNVIGDLPMSNGQTLAQMYPSLQTIYDFL
                     WLIGEAIFFYLPVGICWSAVKKMGGTPILGIVLGVTLVSPQLMNAYLLGQQLPEVWDF
                     GMFSIAKVGYQAQVIPALLAGLALGVIETRLKRIVPDYLYLVVVPVCSLILAVFLAHA
                     LIGPFGRMIGDGVAFAVRHLMTGSFAPIGAALFGFLYAPLVITGVHQTTLAIDLQMIQ
                     SMGGTPVWPLIALSNIAQGSAVIGIIISSRKHNEREISVPAAISAWLGVTEPAMYGIN
                     LKYRFPMLCAMIGSGLAGLLCGLNGVMANGIGVGGLPGILSIQPSYWQVFALAMAIAI
                     IIPIVLTSFIYQRKYRLGTLDIV"
     gene            13330..14985
                     /gene="treC"
                     /locus_tag="NBI74_000811"
     CDS             13330..14985
                     /gene="treC"
                     /locus_tag="NBI74_000811"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HCD1931184.1"
                     /translation="MTHLPHWWQNGVIYQIYPKSFQDTTGSGTGDLRGVIQRLDYLHK
                     LGVDAVWLTPFYVSPQVDNGYDVANYTAIDPTYGTLDDFDELVTQAKSRGIRIILDMV
                     FNHTSTQHAWFREALNKESPYRQFYIWRDGEPETPPNNWRSKFGGSAWHWHAESEQYY
                     LHLFAPEQADLNWENPAVRAELKKVCEFWADRGVDGLRLDVVNLISKDPRYPEDLDGD
                     GRRFYTDGPRAHEFLHEMNRDVFTPHGLMTVGEMSSTSLEHCQRYAALTGSELSMTFN
                     FHHLKVDYPDGEKWTLAKPDFVALKTLFRHWQQGMHNVAWNALFWCNHDQPRIVSRFG
                     DEGEYRVPAAKMLAMVLHGMQGTPYIYQGEEIGMTNPHFTRITDYRDVESLNMFAELR
                     NDGRDADELLAILASKSRDNSRTPMQWTNGDNAGFTAGEPWIGLGDNYQEINVEAALA
                     DESSVFYTYQKLIALRKQEAVLTWGDYQDLLPNSPVLWCYRREWKGQTLLVIANLSRE
                     TQPWQPGKMLGNWQLVMHNYEEASPQPCAMTLRPFEAVWWLQK"
     gene            15379..17517
                     /gene="nrdD"
                     /locus_tag="NBI74_000812"
     CDS             15379..17517
                     /gene="nrdD"
                     /locus_tag="NBI74_000812"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HCD1931185.1"
                     /translation="MTPHVMKRDGCKVPFKSERIKEAILRAAKAAEVDDADYCATVAA
                     VVSEQMQGRNQVDINEIQTAVENQLMSGPYKQLARAYIEYRHDRDIEREKRGRLNQEI
                     RGLVEQTNSSLLNENANKDSKVIPTQRDLLAGIVAKHYARQHLLPRDVVQAHERGDIH
                     YHDLDYSPFFPMFNCMLIDLKGMLTQGFKMGNAEIEPPKSISTATAVTAQIIAQVASH
                     IYGGTTINRIDEVLAPFVTASYNKHRKTAEEWNIPDAEGYANSRTIKECYDAFQSLEY
                     EVNTLHTANGQTPFVTFGFGLGTSWESRLIQESILRNRIAGLGKNRKTAVFPKLVFAI
                     RDGLNHKKGDPNYDIKQLALECASKRMYPDILNYDQVVKVTGSFKTPMGCRSFLGVWE
                     NENGEQIHDGRNNLGVISLNLPRIALEAKGDEATFWKLLDERLVLARKALMTRIARLE
                     GVKARVAPILYMEGACGVRLNADDDVSEIFKNGRASISLGYIGIHETINALFGGEHVY
                     DNEQLRAKGIAIVERLRQAVDQWKEETGYGFSLYSTPSENLCDRFCRLDTAEFGVVPG
                     VTDKGYYTNSFHLDVEKKVNPYDKIDFEAPYPPLANGGFICYGEYPNIQHNLKALEDV
                     WDYSYQHVPYYGTNTPIDECYECGFTGEFECTSKGFTCPKCGNHDTSRVSVTRRVCGY
                     LGSPDARPFNAGKQEEVKRRVKHLGNGQIG"
     gene            17675..18139
                     /gene="nrdG"
                     /locus_tag="NBI74_000813"
     CDS             17675..18139
                     /gene="nrdG"
                     /locus_tag="NBI74_000813"
                     /EC_number="1.97.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate
                     reductase-activating protein"
                     /protein_id="HCD1931186.1"
                     /translation="MNYHQYYPVDIVNGPGTRCTLFVSGCVHECPGCYNKSTWRVNSG
                     QPFTKAMEDQIINDLNDTRIKRQGISLSGGDPLHPQNVPDILKLVQRIRAECPGKDIW
                     MWTGYKLDELNAAQMQVVDLINVLVDGKFVQDLKDPSLIWRGSSNQVVHHLR"
     gene            complement(18184..18570)
                     /gene="cybC"
                     /locus_tag="NBI74_000814"
     CDS             complement(18184..18570)
                     /gene="cybC"
                     /locus_tag="NBI74_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b562"
                     /protein_id="HCD1931187.1"
                     /translation="MRKSLLAILAVSSLVFSSASFAADLEDNMETLNDNLKVVEKADN
                     AAQVKDALTKMRAAALDAQKATPPKLEGKSPDSPEMKDFRHGFDILVGQIDDALKLAN
                     EGKVKEAQAAAEQLKTTRNAYHQKYR"
     gene            complement(18625..19977)
                     /gene="pmbA"
                     /locus_tag="NBI74_000815"
     CDS             complement(18625..19977)
                     /gene="pmbA"
                     /locus_tag="NBI74_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease PmbA"
                     /protein_id="HCD1931188.1"
                     /translation="MALAMKVISQVEAQRKILEEAVSTALELASGKSDGAEVAVSKTT
                     GISVSTRYGEVENVEFNSDGALGITVYHQNRKGSASSTDLSPQAIARTVQAALDIARY
                     TSPDPYAGVADKELLAFDAPDLDLFHPADVSPDEAIELAARAEQAALQADKRITNTEG
                     GSFNSHYGVKVFGNSHGMLQGYCSTRHSLSSCVIAEENGDMERDYAYTIGRAMSDLQT
                     PEWVGADCARRTLSRLSPRKLSTMKAPVIFANEVATGLFGHLVGAIAGGAVYRKSTFL
                     LDSLGKQILPDWLTIEEHPHLLKGLASTPFDSEGVRTERRDIVKDGILTQWLLTSYSA
                     RKLGLKSTGHAGGIHNWRIAGQGLSFEQMLKEMGTGLVVTELMGQGVSAITGDYSRGA
                     AGFWVENGEIQYPVSEITIAGNLKDMWRNIVTVGNDIETRSNIQCGSVLLPEMKIAGQ
                     "
     gene            20071..20622
                     /locus_tag="NBI74_000816"
     CDS             20071..20622
                     /locus_tag="NBI74_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein"
                     /protein_id="HCD1931189.1"
                     /translation="MTKQPEDWLDDVPGDDIEDEDDEIIWVSKSEIKRDAEELKRLGA
                     EIVDLGKNALDKIPLDADLRAAIELAQRIKMEGRRRQLQLIGKMLRQRDVEPIRQALD
                     KLKNRHNQQVVLFHKLENLRDRLIDQGDDAIAEVLNLWPDADRQQLRTLIRNAKKEKE
                     GNKPPKSARQIFQYLRELAENEG"
     gene            complement(20780..22153)
                     /gene="mpl"
                     /locus_tag="NBI74_000817"
     CDS             complement(20780..22153)
                     /gene="mpl"
                     /locus_tag="NBI74_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HCD1931190.1"
                     /translation="MRIHILGICGTFMGGLAMLARQLGHEVTGSDANVYPPMSTLLEK
                     QGIELIQGYDASQLDPQPDLVIIGNAMTRGNPCVEAVLEKNIPYMSGPQWLHDFVLRD
                     RWVLAVAGTHGKTTTAGMATWILEQCGYKPGFVIGGVPGNFEVSARLGESDFFVIEAD
                     EYDCAFFDKRSKFVHYCPRTLILNNLEFDHADIFDDLKAIQKQFHHLVRIVPGQGRII
                     WPENDINLKQTMAMGCWSEQELVGEQGHWQAKKLTTDASEWEVLLDGEKVGEVKWSLV
                     GEHNMHNGLMAIAAARHVGVAPADAANALGSFINARRRLELRGEANGVTVYDDFAHHP
                     TAILATLAALRGKVGGTARIIAVLEPRSNTMKMGICKDDLAPSLGRADEVFLLQPAHI
                     PWQVAEVAEACVQPAHWSGDVDTLADMVVKTAQPGDHILVMSNGGFGGIHQKLLDGLA
                     KKAEAAQ"
     gene            22329..23327
                     /gene="fbp"
                     /locus_tag="NBI74_000818"
     CDS             22329..23327
                     /gene="fbp"
                     /locus_tag="NBI74_000818"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HCD1931191.1"
                     /translation="MKTLGEFIVEKQHEFSHATGELTALLSAIKLGAKIIHRDINKAG
                     LVDILGASGAENVQGEVQQKLDLFANEKLKAALKARDIVAGIASEEEDEIVVFEGCEH
                     AKYVVLMDPLDGSSNIDVNVSVGTIFSIYRRVTPVGTPVTEEDFLQPGNKQVAAGYVV
                     YGSSTMLVYTTGCGVHAFTYDPSLGVFCLCQERMRFPEKGKTYSINEGNYIKFPNGVK
                     KYIKFCQEEDKSTNRPYTSRYIGSLVADFHRNLLKGGIYLYPSTASHPDGKLRLLYEC
                     NPMAFLAEQAGGKASDGKERILDIIPETLHQRRSFFVGNDHMVEDVERFIREFPDA"
     gene            complement(23360..24355)
                     /gene="yjfF"
                     /locus_tag="NBI74_000819"
     CDS             complement(23360..24355)
                     /gene="yjfF"
                     /locus_tag="NBI74_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease YjfF"
                     /protein_id="HCD1931192.1"
                     /translation="MIKRNLPLMITIGVFVLGYIYCLTQFPGFASTRVICNILTDNAF
                     LGIIAVGMTFVILSGGIDLSVGSVIAFTGVFLAKVIGDFGLSPLLAFPLVLVMGCAFG
                     AFMGLLIDALKIPAFIITLAGMFFLRGVSYLVSEESIPINHPVYDTLSSLAWKIPGGG
                     RLSAMGLLMLAVVVIGIFLAHRTRFGNQVYAIGGNATSANLMGISTRSTTIRIYMLST
                     GLATLAGIVFSIYTQAGYALAGVGVELDAIASVVIGGTLLSGGVGTVLGTLFGVAIQG
                     LIQTYINFDGTLSSWWTKIAIGILLFIFIALQRGLTVLWENRQSSPVTRVNIVQR"
     gene            complement(24342..25364)
                     /locus_tag="NBI74_000820"
     CDS             complement(24342..25364)
                     /locus_tag="NBI74_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCD1931193.1"
                     /translation="MPQSLPDTTPPKRRFRWPTGMPQLAALLLVLLVDSLVAPHFWQV
                     VLQDGRLFGSPIDILNRAAPVALLAIGMTLVIATGGIDLSVGAVMAIAGATTAAMTVA
                     GFSLPIVLLSALGTGILAGLWNGILVAILKIQPFVATLILMVAGRGVAQLITSGQIVT
                     FNSPDLSWFGSGSLLFLPTPVIIAVLTLLLFWLLTRKTALGMFIEAVGINIRAAKNAG
                     VNTRIIVMLTYVLSGLCAAIAGIIVAADIRGADANNAGLWLELDAILAVVIGGGSLMG
                     GRFNLLLSVVGALIIQGMNTGILLSGFPPEMNQVVKAVVVLCVLIVQSQRFISLIKGV
                     RNRDKT"
     gene            complement(25378..26880)
                     /locus_tag="NBI74_000821"
     CDS             complement(25378..26880)
                     /locus_tag="NBI74_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HCD1931194.1"
                     /translation="MTTDQHQEILRTEGLSKFFPGVKALDNVNFSLRRGEIMALLGEN
                     GAGKSTLIKALTGVYHADRGTIWLEGQAISPKNTAHAQQLGIGTVYQEVNLLPNMSVA
                     DNLFIGREPKRFGLLRRKEMEKRATELMASYGFSLDVREPLNRFSVAMQQIVAICRAI
                     DLSAKVLILDEPTASLDTQEVELLFGLMRQLRDRGVSLIFVTHFLDQVYQVSDRITVL
                     RNGSFVGCRETRELPQIELVKMMLGRELDTHALQRAGRTLLSDKPVAAFKNYGKKGTI
                     APFDLEVRPGEIVGLAGLLGSGRTETAEVIFGIKPADSGTALIKGKPQTPRSPHQASV
                     LGIGFCPEDRKTDGIIAAASVRENIILALQAQRGWLRPISRKEQQEIAERFIRQLGIR
                     TPSTEQPIEFLSGGNQQKVLLSRWLLTRPQFLILDEPTRGIDVGAHAEIIRLIETLCA
                     DGLALLVISSELEELVGYADRVIIMRDRKQVAEIPLAELSVPAIMNAIAA"
     gene            complement(27093..28049)
                     /gene="ytfQ"
                     /locus_tag="NBI74_000822"
     CDS             complement(27093..28049)
                     /gene="ytfQ"
                     /locus_tag="NBI74_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter
                     substrate-binding protein YtfQ"
                     /protein_id="HCD1931195.1"
                     /translation="MWKRLLVVSAVSAAMSSMALAAPLTVGFSQVGSESGWRAAETNV
                     AKSEAEKRGITLKIADGQQKQENQIKAVRSFVAQGVDAIFIAPVVATGWEPVLKEAKD
                     AEIPVFLLDRSIDVKDKSLYMTTVTADNILEGKLIGDWLVKEVNGKPCNVVELQGTVG
                     ASVAIDRKKGFAEAIKNAPNIKIIRSQSGDFTRSKGKEVMESFIKAENNGKNICMVYA
                     HNDDMVIGAIQAIKEAGLKPGKDILTGSIDGVPDIYKAMIDGEANASVELTPNMAGPA
                     FDALEKYKKDGTMPEKLTLTKSTLYLPDTAKEELEKKKNMGY"
     gene            28359..28889
                     /gene="ppa"
                     /locus_tag="NBI74_000823"
     CDS             28359..28889
                     /gene="ppa"
                     /locus_tag="NBI74_000823"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001739810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HCD1931196.1"
                     /translation="MSLLNVPAGKDLPEDIYVVIEIPANADPIKYEIDKESGALFVDR
                     FMSTAMFYPCNYGYINHTLSLDGDPVDVLVPTPYPLQPGSVIRCRPVGVLKMTDEAGE
                     DAKLIAVPHTKLSKEYDHIKDVNDLPELLKAQIAHFFEHYKDLEKGKWVKVEGWENAE
                     AAKAEIVASFERAKNK"
     gene            complement(29267..29608)
                     /locus_tag="NBI74_000824"
     CDS             complement(29267..29608)
                     /locus_tag="NBI74_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HCD1931197.1"
                     /translation="MRIFVYGSLRHKQGNSHWMTNAQLLGDFSIDNYQLYSLGHYPGA
                     VPGNGTVHGEVYRIDNATLAELDALRTRGGEYARQLIQTPYGSAWMYVYQRPVDGLKL
                     IESGDWLDRDK"
     gene            complement(29611..33390)
                     /gene="tamB"
                     /locus_tag="NBI74_000825"
     CDS             complement(29611..33390)
                     /gene="tamB"
                     /locus_tag="NBI74_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamB"
                     /protein_id="HCD1931198.1"
                     /translation="MSLWKKISLGVVIVILLLLGSVAFLVGTTSGLHLVFKAADRWVP
                     GLDIGKVTGGWRDLTLSDVRYEQPGVAVKAGNLHLAVGLECLWNSSVCINDLALKDIQ
                     VNIDSKKMPPSEQVEEEEDSGPLDLSTPYPITLTRVALDNVNIKIDDTTVSVMDFTSG
                     LNWQEKTLTLKPTSLIGLLIALPKVAEAAQEEVVEPKIENPQPEDKPLGETLKDLFSR
                     PVLPEMTDVHLPLNLNIEEFKGEQLRVTGDTDITVSTMLLKVSSIDGNTKLDALDIDS
                     SQGIVNASGTAQLSDNWPVDITLNSTLNVEPLKGEKVKLKVGGALREQLEIGVNLSGP
                     VDMDLRAQTRLAEAGLPLNVEVNSKQLYWPFTGEKQYQADDLKLKLTGKMTDYAISMR
                     TAVKGQEIPPATITLDAKGNEQQVNLDKLTVAALEGKTELKALLDWQQAISWRGELTL
                     NGINTAKEIPEWPSKLNGLIKTRGSLYGGTWQMEVPELKLTGNVKQNKVNVDGTLKGN
                     SYMQWMIPGLHLELGPNSAEVKGELGVKDLNLDATINAPGLDNALPGLGGTAKGLVKV
                     RGTVEAPQLLADITARGLRWQELSVAQVRVEGDIKSTDQIAGKLDVRVEQISQPDVNI
                     NLVTLNAKGSEKQHELQLRIQGEPISGQLNLAGSFDRKEERWKGTLSNTRFQTPVGPW
                     SLTRDIALDYRNKEQKISIGPHCWLNPNAELCVPQTIDAGAEGRAVVNLNRFDLAMLK
                     PFMPETTQASGIFTGKADVAWDTTKEGLPQGSITLSGRNVQVTQTVNDAALPVAFQTL
                     NLTAELRNNRAELGWTIRLTNNGQFDGQVQVTDPQGRRNLGGNVNIRNFNLAMINPIF
                     TRGEKAAGMVSANLRLGGDVQSPQLFGQLQVTGVDIDGNFMPFDMQPSQLAVNFNGMR
                     STLAGTVRTQQGEIYLSGDADWSQIENWRARVTAKGSKVRITVPPMVRMDVSPDVVFE
                     ATPNLFTLDGRVDVPWARIVVHELPESAVGVSSDVVMLNDNLQPEEPKTASIPINSNL
                     TVHVGNNVRIDAFGLKARLTGDLNVVQDKQGLGLNGQINIPEGRFHAYGQDLIVRKGE
                     LLFSGPPDQPYLNIEAIRNPDATEDDVIAGVRVTGLADEPKAEIFSDPAMSQQAALSY
                     LLRGQGLESDQSDSAAMTSMLIGLGVAQSGQIVGKIGETFGVSNLALDTEGVGDSSQV
                     VVSGYVLPGLQVKYGVGIFDSIATLTLRYRLMPKLYLEAVSGVDQALDLLYQFEF"
     gene            complement(33387..35120)
                     /gene="tamA"
                     /locus_tag="NBI74_000826"
     CDS             complement(33387..35120)
                     /gene="tamA"
                     /locus_tag="NBI74_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamA"
                     /protein_id="HCD1931199.1"
                     /translation="MRYIRQLCCVSLLCLSGSAVAANVRLQVEGLSGQLEKNVRAQLS
                     TIESDEVTPDRRFRARVDDAIREGLKALGYYQPTIEFDLRPPPKKGRQVLIAKVTPGV
                     PVLIGGTDVVLRGGARTDKDYLKLLDTRPAIGTVLNQGDYENFKKSLTSIALRKGYFD
                     SEFTKAQLGIALGLHKAFWDIDYNSGERYRFGHVTFEGSQIRDEYLQNLVPFKEGDEY
                     ESKDLAELNRRLSATGWFNSVVVAPQFDKARETKVLPLTGVVSPRTENTIETGVGYST
                     DVGPRVKATWKKPWMNSYGHSLTTSTSISAPEQILDFSYKMPLLKNPLEQYYLVQGGF
                     KRTDLNDTESDSTTLVASRYWDLSSGWQRAINLRWSFDHFTQGEITNTTMLFYPGVMI
                     SRTRSRGGLMPTWGDSQRYSIDYSNTAWGSDVDFSVFQAQNVWIRTLYDRHRFVTRGT
                     LGWIETGDFDKVPPDLRFFAGGDRSIRGYKYKSIAPKYANGDLKGASKLITGSLEYQY
                     NVTGKWWGAVFVDSGEAVSDIRRSDFKTGTGVGVRWESPVGPIKLDFAVPVADKDEHG
                     LQFYIGLGPEL"
     gene            35327..35965
                     /gene="msrA"
                     /locus_tag="NBI74_000827"
     CDS             35327..35965
                     /gene="msrA"
                     /locus_tag="NBI74_000827"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HCD1931200.1"
                     /translation="MSLFDKKHLVSPADALPGRNTPMPVATLHAVNGHSMTNVPDGME
                     IAIFAMGCFWGVERLFWQLPGVYSTAAGYTGGYTPNPTYREVCSGDTGHAEAVRIVYD
                     PSVISYEQLLQVFWENHDPAQGMRQGNDHGTQYRSAIYPLTPEQDAAARASLERFQAA
                     MLAADDDRRITTEIANATPFYYAEDDHQQYLHKNPYGYCGIGGIGVCLPPEA"
     gene            36288..37631
                     /locus_tag="NBI74_000828"
     CDS             36288..37631
                     /locus_tag="NBI74_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HCD1931201.1"
                     /translation="MLNSILVILCLIAVSAFFSMSEISLAASRKIKLKLLADEGNINA
                     QRVLNMQENPGMFFTVVQIGLNAVAILGGIVGDAAFSPAFHSLFSRYMSAELSEQLSF
                     ILSFSLVTGMFILFADLTPKRIGMIAPEAVALRIINPMRFCLYVCTPLVWFFNGLANI
                     IFRIFKLPMVRKDDITSDDIYAVVEAGALAGVLRKQEHELIENVFELESRTVPSSMTP
                     RENVIWFDLHEDEQSLKNKVAEHPHSKFLVCNEDIDHIIGYVDSKDLLNRVLANQSLA
                     LNSGVQIRNTLIVPDTLTLSEALESFKTAGEDFAVIMNEYALVVGIITLNDVMTTLMG
                     DLVGQGLEEQIVARDENSWLIDGGTPIDDVMRVLDIDEFPQSGNYETIGGFMMFMLRK
                     IPKRTDSVKFAGYKFEVVDIDNYRIDQLLVTRIDSKATALSPKMPDAKDKEESVA"
     gene            complement(37692..37898)
                     /locus_tag="NBI74_000829"
     CDS             complement(37692..37898)
                     /locus_tag="NBI74_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1107 domain-containing protein"
                     /protein_id="HCD1931202.1"
                     /translation="MKIFQRYNPLQVAKYVKILFRGRLYIKDVGAFEFDKGKILIPKV
                     KDKLHLSVMSEVNRQVMRLQTEMA"
     gene            38223..38780
                     /locus_tag="NBI74_000830"
     CDS             38223..38780
                     /locus_tag="NBI74_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001498728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HCD1931203.1"
                     /translation="MTLRKILALTCLLLPMMASAHQFETGQRVPPIGITDRGELVLDK
                     DQFSYKTWNSAQLVGKVRVLQHIAGRTSAKEKNATLIEAIKSAKLPHDRYQTTTIVNT
                     DDAIPGSGMFVRSSLESNKKLYPWSQFIVDSNGVARGAWQLDEESSAVVVLDKDGRVQ
                     WAKDGALTQEEVQQVMDLLHKLINK"
     gene            complement(38770..39510)
                     /gene="cysQ"
                     /locus_tag="NBI74_000831"
     CDS             complement(38770..39510)
                     /gene="cysQ"
                     /locus_tag="NBI74_000831"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HCD1931204.1"
                     /translation="MLDQVCQLARNAGDAIMQVYDGTKPMDVVSKADNSPVTAADIAA
                     HTVIMDGLRTLTPDIPVLSEEDPPGWEVRQHWQRYWLVDPLDGTKEFIKRNGEFTVNI
                     ALIDHGKPILGVVYAPVMNVMYSAAEGKAWKEECGVRKQIQVRDARPPLVVISRSHAD
                     AELKEYLQQLGEHQTTSIGSSLKFCLVAEGQAQLYPRFGPTNIWDTAAGHAVAAAAGA
                     HVHDWQGKPLDYTPRESFLNPGFRVSIY"
     gene            39700..41643
                     /gene="cpdB"
                     /locus_tag="NBI74_000832"
     CDS             39700..41643
                     /gene="cpdB"
                     /locus_tag="NBI74_000832"
                     /EC_number="3.1.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2',3'-cyclic-nucleotide 2'-phosphodiesterase"
                     /protein_id="HCD1931205.1"
                     /translation="MIKFSATLLATLIAASVNAATVDLRIMETTDLHSNMMDFDYYKD
                     TATEKFGLVRTASLINDARNEVKNSVLVDNGDLIQGSPLADYMSAKGLKAGDIHPVYK
                     ALNTLDYTVGTLGNHEFNYGLDYLKNALAGAKFPYVNANVIDARTKQPMFTPYLIKDT
                     EVVDKDGKKQTLKIGYIGVVPPQIMGWDKANLSGKVTVNDITETVRKYVPEMREKGAD
                     VVVVLAHSGLSADPYKVMAENSVYYLSEIPGVNAIMFGHAHAVFPGKDFADIEGADIA
                     KGTLNGVPAVMPGMWGDHLGVVDLQLSNDSGKWQVTQAKAEARPIYDIANKKSLAAED
                     SKLVETLKADHDATRQFVSKPIGKSADNMYSYLALVQDDPTVQVVNNAQKAYVEHYIQ
                     GDPDLAKLPVLSAAAPFKVGGRKNDPASYVEVEKGQLTFRNAADLYLYPNTLIVVKAS
                     GKEVKEWLECSAGQFNQIDPNSTKPQSLINWDGFRTYNFDVIDGVNYQIDVTQPARYD
                     GECQMINANAERIKNLTFNGKPIDPNAMFLVATNNYRAYGGKFAGTGDSHIAFASPDE
                     NRSVLAAWIADESKRAGEIHPAADNNWRLAPIAGDKKLDIRFETSPSDKAAAFIKEKG
                     QYPMNKVATDDIGFAIYQVDLSK"
     gene            complement(41772..42152)
                     /locus_tag="NBI74_000833"
     CDS             complement(41772..42152)
                     /locus_tag="NBI74_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HCD1931206.1"
                     /translation="MSQVSLSQQLKEGNLFAEQCPSREVLKHVTSRWGVLILVALREG
                     THRFSDLRRKMGGVSEKMLTQSLQALEQDGFLNRIAYPVVPPHVEYSLTPLGEQVSEK
                     VAALADWIELNLPEVLAVRDERAA"
     gene            42241..43101
                     /gene="qorB"
                     /locus_tag="NBI74_000834"
     CDS             42241..43101
                     /gene="qorB"
                     /locus_tag="NBI74_000834"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HCD1931207.1"
                     /translation="MIAITGATGQLGHYVIESLMKTVPASQIVAIVRNPAKAQALTAQ
                     GITVRQADYGDEAALTSALQGVEKLLLISSSEVGQRAPQHRNVINAAKAADVKFIAYT
                     SLLHANSSPLGLADEHIETEKMLADSGIVYTLLRNGWYSENYLASAPAALEHGVFIGA
                     AGDGKIASATRADYAVAAARVISEAGHEGKVYELAGDRAWTLTQLAAELTKQSGKSVT
                     YQNLSEADFAAALKSVGLPDGLADMLADSDVGASKGGLFDDSKTLSKLIGRPTTTLAE
                     SVSHLFNVNN"
     gene            43210..44175
                     /locus_tag="NBI74_000835"
     CDS             43210..44175
                     /locus_tag="NBI74_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418631.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HCD1931208.1"
                     /translation="MISGVLYALLAGLMWGLIFVGPLIVPEYPAMLQSMGRYLALGLI
                     ALPIAWLGRVRLRQLARRDWLTALMLTMMGNLIYYFCLASAIQRTGAPVSTMIIGTLP
                     VVIPVFANLLYSQRDGKLAWGKLAPALVCIGIGLACVNIAELNHGLPDFDWARYTSGI
                     VLALVSVVCWAWYALRNARWLRENPDKHPMMWATAQALVTLPVSLIGYLVACYWLNMQ
                     TPDFSLPFGPRPLVFISLMVAIAVLCSWVGALCWNVASQRLPTVILGPLIVFETLAGL
                     LYTFLLRQQMPPLMTLSGIALLVIGVVIAVRAKPEKPLTESVSES"
     gene            44283..44945
                     /gene="ytfE"
                     /locus_tag="NBI74_000836"
     CDS             44283..44945
                     /gene="ytfE"
                     /locus_tag="NBI74_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair protein YtfE"
                     /protein_id="HCD1931209.1"
                     /translation="MAYRDQPLGELALSIPRASALFRKYDMDYCCGGKQTLARAAARK
                     ELDVDVIEAELAKLAEQPIEKNWRSAPLAEIIDHIIVRYHDRHREQLPELILQATKVE
                     RVHADKPSVPKGLTKYLTMLHEELSSHMMKEEQILFPMIKQGMGSQAMGPISVMESEH
                     DEAGELLEVIKHTTNNVTPPPEACTTWKAMYNGINELIDDLMEHISLENNVLFPRALA
                     GE"
     gene            complement(44990..46402)
                     /gene="cycA"
                     /locus_tag="NBI74_000837"
                     /gene_synonym="dagA"
     CDS             complement(44990..46402)
                     /gene="cycA"
                     /locus_tag="NBI74_000837"
                     /gene_synonym="dagA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine/D-alanine/glycine transporter"
                     /protein_id="HCD1931210.1"
                     /translation="MVDQVKVVADDQAPAEQSLRRNLTNRHIQLIAIGGAIGTGLFMG
                     SGKTISLAGPSIIFVYMIIGFMLFFVMRAMGELLLSNLEYKSFSDFASDLLGPWAGYF
                     TGWTYWFCWVVTGMADVVAITAYAQFWFPGLSDWVASLSVIILLLVLNLATVKMFGEM
                     EFWFAMIKIVAIVSLIVVGLVMVAMHFQSPTGVEASFAHLWNDGGWFPKGLSGFFAGF
                     QIAVFAFVGIELVGTTAAETKDPEKSLPRAINSIPIRIIMFYVFSLIVIMSVTPWSSV
                     VPEKSPFVELFVLVGLPAAASVINFVVLTSAASSANSGVFSTSRMLFGLAQEGVAPKA
                     FAKLSKRAVPAKGLTFSCICLLGGVVMLYVNPSVIGAFTMITTVSAILFMFVWTIILC
                     SYLVYRKQRPHLHEKSIYKMPLGKLMCWVCMAFFVFVLVLLTLEDDTRQALLVTPLWF
                     IALGLGWLFIGKKQAAELRK"
     gene            complement(46711..47331)
                     /gene="fklB"
                     /locus_tag="NBI74_000838"
     CDS             complement(46711..47331)
                     /gene="fklB"
                     /locus_tag="NBI74_000838"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015742582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HCD1931211.1"
                     /translation="MTTPTFDTIEAQASYGIGLQVGQQLSESGLEGLLPEALVAGIAD
                     ALEGKHPAVPVDVVHRALREIHERADAVRRQRFQAMAAEGVKYLEENAKKEGVNSTES
                     GLQFRVINQGEGAIPARTDRVRVHYTGKLIDGTVFDSSVARGEPAEFPVNGVIPGWIE
                     ALTLMPVGSKWELTIPQELAYGERGAGASIPPFSTLVFEVELLEIL"
     gene            47549..48187
                     /gene="ytfB"
                     /locus_tag="NBI74_000839"
     CDS             47549..48187
                     /gene="ytfB"
                     /locus_tag="NBI74_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YtfB"
                     /protein_id="HCD1931212.1"
                     /translation="MPGRFELKPTLEKVWHAPDNFRFMDPLPPMHRRGIIIAAIVLVV
                     GFLLPSDDTPNAPVVTREAQLDIQSQSQPPTEEQLRAQLVTPQNDPDQVAPVAPEPIQ
                     EGQPEEQPQTTHTQPFQPDSGIDNQWRSYRVEPGKTMAQLFRDHGLPATDVYAMAQVE
                     GADKPLSNLQNGQMVKIRQNASGVVTGLTIDTGNNQQVLFTRQPDGSFIRAR"
     gene            complement(48171..48818)
                     /locus_tag="NBI74_000840"
     CDS             complement(48171..48818)
                     /locus_tag="NBI74_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709992.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HCD1931213.1"
                     /translation="MYLDTSNVQSLKEKLLLCAVNEFAEYGYEGARVDNIVKAAGCSK
                     QTVYHHFGNKENLFIEVLEYTWNDIRQKEKALDFSDLPPQKAIEKIIDFTWDYYISNP
                     WFLKIVHSENQSKGVHYAKSQRLLEINHAHLQLMESLLDEGKKQNIFKPDIDPLQVNI
                     NIAALGGYYLINQHTLGLVYHISMVSPQALEARRKVIKETILSWLLVDPSSTARE"
     gene            49031..49822
                     /locus_tag="NBI74_000841"
     CDS             49031..49822
                     /locus_tag="NBI74_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-ketoacyl-ACP reductase"
                     /protein_id="HCD1931214.1"
                     /translation="MKSTRPVAVITGAARGIGKGCALELARGGFNLLINDRPDADSVE
                     KLHATQQECLAEGVEVICFPADVGDLSLHEEMLDAAQNQWGRLDCLLNNAGISVKKRG
                     DLLDLEPDSFDQNIAINTRAPFFLAQAFSKRLLAQPKPEAELPHRSIIFVSSINAIML
                     AMNRGEYTIAKTAVSAAARLFAARLCNEQIGVYEVRPGLIKTDMTIPATAYYDELIAK
                     GLVPWGRWGYPADIASTVRAMAEGKLIYTCGQAVAIDGGLSMPRF"
     gene            49832..50674
                     /locus_tag="NBI74_000842"
     CDS             49832..50674
                     /locus_tag="NBI74_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001205218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HCD1931215.1"
                     /translation="MRDLLQRPDLFSINTATLGYKTPLPAIIDACAARGIGAIAPWRR
                     ELQSEDLQQTARQLAASNMNVSGLCRSTYYTAPTLAERKLAIDDNRRALDDAAVLNAA
                     CYMQVVGGLPMGTKDLYEAREQVKQGIRQLLPHSKEVGVPIALEPLHPMTAANRSCLC
                     TLRQALDWCDELDPDGEFGLGVAVDVYHVWWDPDLASQILRAGKRILAFHVSDWLVPT
                     TDLVNDRGMPGDGVINIPSIRRLVENAGFNGAIELEIFSPYWWQKDINSTLDISVDRI
                     AHYC"
     gene            50684..51460
                     /locus_tag="NBI74_000843"
     CDS             50684..51460
                     /locus_tag="NBI74_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001283531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HCD1931216.1"
                     /translation="MNDQPVLFSRAAASCRLTLNREDKCHAINEEMIESLDHYLNEIE
                     NDTALRLVELTATGDKFFCAGGDIKSWSAYSPLDMGRKWIKRGNDVFNRLRNLPQLTV
                     ANLNGHTIGGGIELALCCDIRIARPGAKFSNPEVMLGMVPGWMGIERVLNQVGPVVGR
                     QMLMLGKRLTAQEAQTANLIDEVVEKEQVESWMANQLAQLEKCGPVALAHIKQLILAL
                     ENKHADYSHQLLAGLMSATQDCQQATHAFAEKAGVSFHNQ"
     gene            51470..53011
                     /locus_tag="NBI74_000844"
     CDS             51470..53011
                     /locus_tag="NBI74_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001226973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl CoA:acetate/3-ketoacid CoA transferase"
                     /protein_id="HCD1931217.1"
                     /translation="MRKLSTAETLAAQIQDGATIAISGNGGGMVEADHILAAIEARFL
                     QTGHPRDLTLIHSLGIGDRDSKGTNRFAHAEMLKRIIAGHFTWSPKMQELVKNNAIEA
                     YCFPGGVIQALLREIGAGRPGLFTHVGLGSFVDPRNGGGKSNECTTDDLVELIEIDGE
                     TKLRYRPFKVDYAILRGTYADPRGNVSLEEEAIDMDSYSMALAAHNSGGKVFVQVRDV
                     LEAGTIEPRRVKLPGILVDGIVEHREQPQTYLGGYDLTISGQHRRLSSNDAIELVSHP
                     VRRLIARRAARELVAGASTNFGFGIPGGIPGVALREGVPYQSLWLSVEQGVHNGMMLD
                     DALFGCARNADAIIPSLDQFEFYSGGGIDITFLGMGEMDQYGNVNVSHLNGNLIGPGG
                     FLEIAQNARKVVFCGTFDAKGSKIDVTPDGLHIAQSGQIPKLVTQVEKITFSAAYAQQ
                     SGQEVLYITERAVFQLTAEGVELIEIAPGVEIERDILPYMAFRPIIRHPRLMESSLFM
                     PMEDA"
     gene            53008..55080
                     /locus_tag="NBI74_000845"
     CDS             53008..55080
                     /locus_tag="NBI74_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001591423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HCD1931218.1"
                     /translation="MSQLDDTILDALTHVTFPKGFAQAEPAWVVTVDGVDYPLWKTDA
                     LVVGSGAAGLRAAVELKRRQQNVLIATAGLYMGTSACSGSDKQTLFTAATAGNGDNFT
                     KLAEALASGGAMDHDTAYVEAVGSLHTLGGLQYLGLELPEDRYGAILRYQTDHDEAGR
                     ATSCGPRTSRLMVKVLLEEVQRLAIPMLTSATVIKLLHQRDENGEDRVAGAILATGHR
                     AHNPWGLAIVTAPNVVLATGGPGELYRDSVYPHKCFGSLGLALEEGLTLTNLTESQFG
                     IGTPRSTFPWNLSGTYVQVIPYIYSVDAEGNEYNFLADYYRTTQELASNIFRKGYQWP
                     FHATRVMDFGSSLLDMAVAQEQQSGRQVFMDFNRNPEAVPGDLPFSLDRLDDDVRTYL
                     ENNDALAPSPIERLQRMNPLSISLYKMHGYDLTTQPLQFAMNNQHMNGGIEVDIWGQT
                     SLPGCFAVGEVAGTHGVTRPGGAALNAGQVFAVRLARFIGCTQKRNIDGDIAQLAAPA
                     LASIREIITQAHDNGTGMPLSVVREKIQARMSDHAGFICHADKVRRATRDALLLNEFV
                     QRHGLAIKHVGEVAELFMWRHMTLTSAAVLTQLTHYIDAGGGSRGARMVIDPQGKCLP
                     QTRRGAKEEWRFRSELAEDKNHKLTIQYSQGSFITEVKSLRMQPCINGIYFEKNWPDF
                     LKGDIYTQ"
BASE COUNT        13477 a        14512 c        13965 g        13166 t
ORIGIN      
        1 gattattaat atcaccgctg gtgtgaaagt cgtcacattt tctggaagaa ataaactgaa
       61 tcattgcatc tgaaaattac tgaacagacc tgactacaag catttaattt gtttgcagcc
      121 atctcctctt tatttttgaa aaagtgttaa aggagtaatg atggaaaagc attatgttgg
      181 ttctgaaatt ggacaactac gcagcgttat gttgcatcgc ccaaatctca gtctgaaacg
      241 tctaacacca tccaattgcc aggagttgtt atttgacgat gtattgtccg ttgagcgggc
      301 aggtgaagag catgatattt tcgccaacac gctgcgccag caagggatag aagttctgtt
      361 actcactgat ttactaacac aaacattgga tatcccggaa gccaaaagct ggttactgga
      421 aacacaaata tcagattacc gactcggccc tacgtttgct actgacgttc gcacctggct
      481 tgcagagatg tcacaccggg accttgctcg tcatttaagt ggtgggctga cttatagtga
      541 aattcccgcc tcaattaaaa atatggtggt tgatacccac gatattaatg atttcattat
      601 gaagccgtta ccgaatcatt tatttacgcg tgatacctca tgctggattt ataacggtgt
      661 atcgattaat ccaatggcaa aacctgctcg ccaacgtgaa accaataatc tacgcgctat
      721 ttatcgctgg catccacaat ttgctggcgg cgaatttatt aaatatttcg gcgacgagaa
      781 tattaattat gaccacgcca cgttagaagg tggtgatgtg ctggtgattg gtcggggggc
      841 cgtgttgatc ggcatgtctg aacgcaccac gccacaagga gtcgagtttc ttgctcaggc
      901 attgtttaaa catcgtcagg ctgaacgggt aatcgctgtt gagctgccta aacaccgctc
      961 ctgtatgcat cttgacaccg ttatgactca tatcgatatc gacaccttct cggtttatcc
     1021 cgaagttgtg cgcccggatg ataattgctg gacgctaaca cctgacggtc acggcggcct
     1081 gaaacgtact caagaaagca cactcctcca tgctatcgaa aaagccctcg gtattgacca
     1141 ggtacgttta atcaccacgg ggggtgatgc ctttgaggca gaacgcgaac agtggaatga
     1201 cgctaacaat gtgctcacgc ttcggccggg agtggtagtg ggctacgagc gcaacatctg
     1261 gactaacgaa aaatatgaca aagcaggcat caccgtttta cccatccccg gtgatgaatt
     1321 aggccgcgga cgtggcggcg cacgttgcat gagttgccct ttgcatcgtg acggtatcta
     1381 aggaagagat catggaaaac aaaccaacac tggttatcgc tctcggcggt aatgccctgc
     1441 ttaaacgagg cgaaccactc gaagcagaaa tccaacgtaa aaatatcgat cttgcggcta
     1501 aaactatcgc ccaactgact caacactggc gggttgtact ggttcacggg aatggcccac
     1561 aagttggact tctggcactt caaaatagcg cctatgccca tgtcgctccc tatccactcg
     1621 acattctggg ggccgaaagc caggggatga tcggctacat gctccaacag gcgctgaaaa
     1681 atcaactacc acaacgtgaa atcagcgtgc tgctgacgca ggttgaagtg gatgccaacg
     1741 atcctgcctt cagtaaccca actaagtaca tcggcccaat ttatgaccac gctcagactc
     1801 aggtactaca ggcagaaaaa ggctgggttt tcaaagcaga tggtcactct ttccgccggg
     1861 tcgtaccttc tccacaacct aaacgcatcg tcgagcggga tgccatccag acactgatcg
     1921 cccacgatca tctggtgatc tgtaatggtg gcggtggtgt tccggtggtt gaaaaagccg
     1981 atggatacca tgggattgaa gcggttatcg acaaagatct ttcagccgcg ctactggcaa
     2041 gtcagatcca tgctgacgcc ttgttgatcc tcactgatgc ggacgcagta tatctcgact
     2101 ggggcaagcc aacccaacgc ccgctggccc aggtcacgcc ggaattactc aacgaaatgc
     2161 agtttgatgc cgggtctatg gggccgaaag tcaccgcctg cgcaaagttt gttagtcagt
     2221 gccggggcat tgcagggatc ggctctcttg cagatggtcc ggaaattctc gccggagaca
     2281 aaggcactct tattcgcctt gatacccccc ataaccacgc ttgatccatt tttataagga
     2341 catccccatg gctacttcac tgaaaaaccg taattttctt aagctgcttg attacacccc
     2401 agcagagatt caatacctga ttgatttggc tatcaatctt aaagccgcca agaaatctgg
     2461 aaacgaaaaa caaacgctgg tcggtaaaaa cattgcgttg atttttgaaa aatcctctac
     2521 ccgtacccgc tgtgcctttg aagtcgcggc ctttgatcaa ggtgcgcagg tgacttacat
     2581 cggccccagc ggctcacaaa tcggccataa agaatcaatg aaagataccg ctcgcgtact
     2641 cggacgcatg tatgacggta ttgagtatcg cggttatggt cagaatatcg ttgaagaatt
     2701 aggtgagttc gctggtgttc cggtatggaa cggactgacc aatgaatttc acccgacaca
     2761 gatcctcgcg gatttaatga ccatgctgga acatgcgcca ggcaaaacgc tgccagagct
     2821 gagctttgcg tatcttggtg atgcccgaaa taacatgggg aactcgctga tggttggtgc
     2881 tgcaaaaatg gggatggata tccgtctggt ggcaccaaaa tctttctggc cagatgaaac
     2941 gctggtaaca caatgccgtg agatcgccag cgtgactggg gcgagaatca ccctgacaga
     3001 ggatgtggaa gaaggcgtat acgacgttga ttttctctat acggatgttt gggtttcgat
     3061 gggagagcca aaagaagcct gggcagaacg agtgagtctg atggcacctt atcagatcaa
     3121 tcaacaggtg attactgcca cgcgtaaccc tgaagttaaa tttatgcact gcctgcccgc
     3181 cttccataat gagcacacca cagtaggacg cgagatcgag atggcctacg gcctcaaagg
     3241 actggaagtt actgaagagg tttttgaatc ggcccactct attgttttcg atgaagcaga
     3301 aaaccggatg cacaccatta aagcggtgat ggtagcaact ctcggcgact aaccctgacg
     3361 ggcatgcgtc gacgagtagc gtatgcccgt gattatggag atcacattat gggtaaattc
     3421 aaatttccca ccgcttatac catcttgttc gtgctgattg cgttagttgc ggcaatgacc
     3481 tggattgtac ctgcgggaaa atatcagatg gtgacaaatg ccgcactggg aaaggaagtt
     3541 cctgttgcag gcacttacgc acctgttgag gcacaaccac agggtattac ggctgtcctg
     3601 ttagcgccca tcgacggctt gtacaaccat caaacctaca ctgcgggagc gattgatgtc
     3661 gcgctgtttg tcctgatcat cggtggattt ttgggagtag taaataaaac cggggcaata
     3721 gatgccggta tcgaacgagt gacaactcgc cttaatggtc gggaagagtg gatgattccg
     3781 atcctgatgg cgctcttcgc tgctggcggt actatttatg ggatggcgga agaatcactc
     3841 cccttctaca cgttgctggt gcccgtcatg atggctgccc gctttgatcc tctggtcgct
     3901 gccgccacag tgttattggg atcgggcatt ggcacgctgg gctcaaccat taacccattt
     3961 gctaccgtca ttgctgccaa cgccgcaggg atccccttta ctcagggcat tttgctgcgt
     4021 gttattttgc tgttagtagg ctacgtgatt tgtgtttact gggttatgcg ttatgcccgc
     4081 aaagttcgca acagtccgga aagctctatc gtggcggata agatggctga aaaccaggcg
     4141 cattttctgg gaaatcgttc agaaacgatg ctggaattta cccccacgcg caaagcgatc
     4201 ctgatacttt ttgctgcatc ctttgccttt atgatttacg gcgtagcggt actgggttgg
     4261 tggatggcgg aaatctctgc tgtctttctg gcagctgcgg tcatcgtggg tgtcattgcc
     4321 cgcatgggcg aagaaacctt caccagtacc tttattgacg gcgcgcggga tctccttggt
     4381 gtggcactga ttatcggcat cgcccgcggt attgtggtcg tgatggataa cggcatgatt
     4441 acgcacacaa ttctgcacag tgctgagaat ctggtttcag ggctttccac cactgttttc
     4501 atcaacgtga cctattggct ggaagtattg ctctcttttc tggttccctc atcttccggc
     4561 cttgcagtgt taacgatgcc aataatggct ccactcgctg attttgctca tgtacaacgc
     4621 gatctggtgg tgaccgctta tcagtctgct tccggggtgg ttaatttgat tacgccaacc
     4681 tctgcggtag tgatgggcgg tctggcgatt gcccgtgtac cctgggtccg ttatctgaga
     4741 tgggtagcac cgctactgct gattctcacg atgttgaata tgattgtgct gagtatcgcc
     4801 gcgatgattt aacgcgacag gccgcttatc aaagcggcct tcaaatctgg tgtagtgata
     4861 aatgaaccac tctcacatta taaaatgtga aaaatatcat caggtaataa taaataaaag
     4921 gtgcattatg aaggaatacg atgattactc cgccaaagaa aagaagcaac tggcggtctg
     4981 tcagcgcctg attaccgaaa aaagttatct ttcacaggag gaaatccgcc gcgatttgca
     5041 aaaccatggc tttgacagta tcagtcagtc aaccgtttca cgactactga aattgctggg
     5101 agtcataaaa attagaaata caaaaggaca aaaaatttat tcggtgaatc ctcaattact
     5161 tcccacacct gacgcaggtc gatccgtcgc tgaaatggtc ctgagtgtcg agcataatgg
     5221 tgaatttatc cttatccata ccgtcgccgg atatggtcgg gcagtagcgc gaatccttga
     5281 tttccatgct ttgccagaaa ttcttggcgt gattgctggc agcaatatcg tttgggtcgc
     5341 accacgtgtg gtaaagcgta cagccctggt gcacaagcaa attaattact tattaaaatt
     5401 gaatatatat tcatgataac cgtttgcctt gctttataac tgcatcatct tcttgatcct
     5461 ctctcagagc tgagtataat gccggacaat ttgccgggag gatgtatggt tcagtgtgtt
     5521 cgacattttg tcttaccgcg tctgaaaaaa gacgctggcc tgccgttttt cttcccgttg
     5581 atcacccatt cccagcccct caatcgaggg gctttttttt gcccaggcgt caggagataa
     5641 aagatggcta atccgctata tcagaaacat atcatttcca taaacgacct tagtcgcgat
     5701 gaccttaatc tggtgctggc gacagcggcg aaactgaaag cgaacccgca accagaactg
     5761 ttgaagcaca aagttattgc cagctgtttc ttcgaagcct ctacccgcac ccgcctctct
     5821 ttcgaaactt ccatgcaccg cctgggtgcc agcgtggtgg gcttctccga cagcgccaat
     5881 acatcactgg gtaaaaaggg cgaaacgctg gccgatacca tttcggttat cagcacttac
     5941 gtcgatgcga tagtgatgcg tcatccgcag gaaggtgcag cgcgcctggc caccgagttt
     6001 tccggcaatg taccggtact gaatgccggt gatggctcca accaacatcc gacgcaaacc
     6061 ttgctggact tattcaccat tcaggaaacc caggggcgtc tggacaatct ccacgtcgca
     6121 atggttggtg acctgaaata tggccgcacc gttcactccc tgacccaggc gctagcgaag
     6181 ttcgacggca atcgttttta ctttatcgcg ccggatgcgc tggcaatgcc gcaatacatt
     6241 ctggatatgc tcgatgaaaa agggatcgcc tggagtctgc acagctctat tgaagaagtg
     6301 atggcggaag tagacatcct gtacatgacc cgcgtgcaaa aagagcgtct ggacccgtcc
     6361 gagtacgcca acgtgaaagc acagtttgtt cttcgcgcca gcgatctcca caacgccaaa
     6421 gccaatatga aagtgctgca tccgctgccg cgtgttgatg agattgcgac ggatgtggat
     6481 aaaacgcctc acgcctggta cttccagcaa gcaggcaacg ggattttcgc tcgccaggcg
     6541 ttactggcac tggttctgaa tcgcgatctg gtactgtaag gggaaataga gatgacacac
     6601 gataataaat tgcaggttga agctattaaa cgcggcacgg taattgacca tatccccgcc
     6661 cagatcggtt ttaagctgtt gagtctgttc aagttgaccg aaacagatca gcgcatcacc
     6721 attggtctga atttgccttc tggcgagatg gggcgcaagg atctgatcaa aatcgaaaat
     6781 acctttttaa gtgaggatca ggttgatcag ctggcgttat atgcaccgca agccacggtt
     6841 aaccgtatcg acaactatga agtggttggc aaatcgcgcc ccagtctgcc agagcgcatc
     6901 gacaatgtgc tggtctgccc gaacagcaac tgtatcagcc atgccgaacc ggtttcatcc
     6961 agctttgccg tgcgaaaacg cgccaatgat atcgcgctca aatgcaaata ctgtgaaaaa
     7021 gagttttccc ataatgtggt gctggccaat taattgcggt tggtaataaa agtctggctc
     7081 cctataatga gccagacttt ttaccgctgt aataaaggag aaatcatgag caaaactatc
     7141 gcgacggaaa atgcaccggc agctatcggt ccttacgtgc agggcgttga tctgggcaat
     7201 atgattatca cttccggtca gatcccggta aacccgaaaa cgggcgaagt cccggcagat
     7261 gtcgctgcac aggcgcgtca gtcgctggat aacgtaaaag cgatcgtcga agccgctggc
     7321 ctgaaagtgg gcgatatcgt taaaaccacc gtgtttgtga aagatctgaa cgacttcgca
     7381 accgttaatg ctacctacga agccttcttc accgaacaca acgccacctt cccggcacgt
     7441 tcttgcgttg aagttgcccg tctgccgaaa gacgtgaaga ttgagatcga agcgatcgct
     7501 gttcgtcgct aatcttgatg gaaatccggg ctatcatgcc cggattaagt ctaatgacaa
     7561 acgcaaaatt gcctgatgcg ctacgcttat caggcctacg tgattcctgc aatttattga
     7621 atttgtaggc ctgataaggc atttacgccg catccggcat gaacaaagct cactttgtct
     7681 gcaatctgat tccgggccac catgcccagt ttattcttta ttgccagccg taacgacggc
     7741 tatagaaccc tttcaccaac tgggttaacg tcatataccc tgccagaatc gcaaccagcc
     7801 acgggaaata gcttaacggc agcgcctgca gttgcagata actggccagc ggtgaaaaag
     7861 gcaatgcgat cccgacaatc atcacgatca cggtcatgat cattaacggc cacgatgcac
     7921 agctctgaat aaacggcaca cggcgggtgc ggatcatatg cacaatcagc gtttgcgaca
     7981 gtaagcccac cacaaaccag cccgactgga acagcgtttg cgtttccggc gtgttggcat
     8041 ggaataccca ccacatcagg caaaacgtca gaatatcgaa aatcgagctg atcggtccga
     8101 agaagaccat aaagcgcccc agatccgccg gattccaacg ctgcggcttt tgaatttgct
     8161 cgtcgtcgac gttatcaaat gggatcgcca cctgtgacac atcgtacagc agattttgaa
     8221 taagcaagtg tagcggcaac atcggcagga agggcaagaa agcactcgcc accagcacgc
     8281 tgaacacatt accgaagtta gagctcgccg tcattttgat gtatttcagc atgttggcga
     8341 aagtgcgacg tccctcaata accccctctt ccagcaccat caggcttttt tccagcagga
     8401 tgatatcagc cgcttcacgg gcaatatcta ccgcgccgtc cacagaaatg ccgatatccg
     8461 cagcgcgtaa ggcgggcgca tcattaatac catcgcccat aaaaccaacc acatgccctt
     8521 cgcgcttcag caacgttacg atgcgttctt tatgcatcgg cgtcagacga gcaaacagcg
     8581 tggtgcgctg tgcgagattt gccagttcgt cgtcagatag cgtttcaata tcactaccaa
     8641 tgaccacctc tcccgcgtcc agccccactt catggcacac tttcgctgca accaactcac
     8701 tgtcgccagt aaggattttt acggtaattc cactcgcttt taatgccttc agtgccggag
     8761 ctgttgtctc ttttggcgga tcaaggaaag caatatatcc ttcgaggatc aggtcggatt
     8821 catccgcccg ctggtaatct ccttcacgcg ctggcaggta tttcgtcgcc accgcaacca
     8881 cgcgcagccc ctgacgattc agcgtatcag taacccgctt aatcttacgc agcatggtgt
     8941 catcgagcgg tacaatctcg ccattgtgac gcacttgcga acacacattg aggatttcct
     9001 gcaatgcgcc tttgtaaacc aactgatgat gttcggtatt ttctgccaca accaccgaca
     9061 tccgacgacg ctcgaaatca aacggaatct catcaatttt ttgccaacgg ctggccagcg
     9121 agcgcgctga ctcttcatcc gtaccttcga gcaccgctgt atcaagcagg tttttaagcc
     9181 cggtctggta atggctgttc aaccacgcgc tatgcagcac gcgttcgcta gttttaccgg
     9241 agatatcggt atgattctcc agcacaattt tatcctgtgt cagggtgccg gttttatcag
     9301 tgcacagaat atccattgcg ccaaagttct gaatggcatc cagatgtttg acgatcactt
     9361 tctgtttcga caacttgact gcgccacgcg ccagcgtcga ggtaacaatc atcggcaaca
     9421 tttctggcgt taagcctacc gcgaccgaaa gcgcaaacag tgcagcttcc caccagtcgc
     9481 ctttagtgta accattgata agcaacacca ccggagccat caccagcata aagcgaatca
     9541 gcagcatact gacgcggcta atcccttgct gaaaggcatt cggctcgctt tcctgctcac
     9601 taacacgacc cgccagttga ccaaaccagg tattggcacc tgtggcaatc accattgctt
     9661 gtgccgtgcc gctcaccacg gtggtgccca taaaacacag ggtgtcgcac tccagcggat
     9721 tgctgtgctc cggctggcga gtggtagcgg ctttttccac gggcagagac tcaccggtta
     9781 acgacgcctg agcgacaaac agatcccgcg cctgcaagat acgtaaatct gccgggatca
     9841 tatcccccgc cgccagtttt ataatatcgc cgggcaccag ctggtctatc gggatctcca
     9901 gccagccatt ttcgcctttg tcgttaatta cgcgcagcac cgtcgcagta ttgctgacca
     9961 tcgctttcag ggcatctgcc gctttggtgg aacgagcttc ctgaataaag ttcagcaacg
    10021 tagaaatagc gaccattagc gcgataacac ctgcggcaaa taaatcttcc gtggcgtaag
    10081 aaatagcacc aagaatggtg agcaaaatat taaaggggtt gcgatagcag acccataaat
    10141 gtacccacca cggcgacggt tgttgtgcgg gtaatttatt ttcaccatgt tgttcgcggg
    10201 cagattccac ttccgccaga tttaaccctt ccggatgggt gtcgaacgtt ttccacaatt
    10261 cttcttcggg catcaccgcc attttcaggc aatgcgcact taaagaaggc gggaccgccg
    10321 cattcactgt ctgctgcgcg ccaggcaatg gatcacgatg aaccagacgg gaaggtaaat
    10381 ggcgaatgag ccgggtaaaa atttctttaa acataaggag tccctccgca ctgtctgaaa
    10441 cagtacagaa atgtcttcag gcgtggcgaa gccttacctg aaggtaataa agctgagcag
    10501 ggacgttaaa acgtcacggc cttacgcatc cggtaacgcc gtgaaaagac gggcttaccg
    10561 gaaaagtttt aatctccgtc gagggagagg cgtgggatca ggttccatat aacctccggt
    10621 aagtgaaaaa agcgccgcaa ataataagga gaatccaata atattcgtag catttatggc
    10681 gatataatac ctgctggcaa ctaaaccaac gataaaccag actttaccca ttgctgaatg
    10741 cacaggtaaa gttaggctca aataattaaa caacacgtta tgggacaaca ggatgcaaaa
    10801 tcggctgacc atcaaagaca tcgcgcgctt aagcggcgtg gggaaatcta cggtttcccg
    10861 ggtgctgaat aacgaaagcg gcgtgagcca gcgcacgcgc gagcgtgttg aagcagtgat
    10921 gaatcagcat ggattttccc cttcccgctc tgcgcgcgct atgcgtgggc aaagcgataa
    10981 agtggtcgcc atcattgtta cccgtctgga ttcgttgtca gaaaatctcg ccgttcaaac
    11041 catgctgcca gcgttctatg aacaaggtta cgatccaatc atgatggaaa gtcagttttc
    11101 cccacaatta gttgccgaac atttgggggt gctgaaacgg cgtaatatcg acggcgtagt
    11161 gctgttcggt tttaccggca taacagaaga aatgttagcc cactggcagt catcgctggt
    11221 tctgctggcg cgtgacgcaa aaggctttgc ttcggtctgt tatgacgacg aaggggcaat
    11281 caagatcctg atgcaacggc tgtatgacca gggacatcgt aatatcagct atctcggcgt
    11341 gccgcacagt gacgtgacaa ccggtaagcg acgtcacgaa gcctacctgg cgttctgcaa
    11401 aacgcataaa ttgcatcccg ttgccgctct gccagggctt gctatgaagc aaggctatga
    11461 gaacgtagca aaagtgatta cgcctgaaac taccgcgtta ctgtgcgcaa ccgacacgct
    11521 ggcacttggc gcaagtaaat acctgcaaga gcaacgcatc gacaccttgc aactggcgag
    11581 cgtcggtaat acaccgttaa tgaaattcct ccatccggag atcgtcaccg tagatcccgg
    11641 ttatgccgaa gctggacgcc aggcggcctg ccagttgatc gcgcaggtaa ccgggcgcag
    11701 cgaaccgcaa caaatcatca tccccgccac cctgtcctga tcgtttcctg aacgataaat
    11761 tgtgatcttc gctgcgtttc gggaacgttc ccgtttttaa atttttccgc gcaatatatt
    11821 ctgcagccaa ccaaaaatgt catctgccat ggggctttat gatgagcaaa ataaaccaaa
    11881 cggatatcga tcggttgatt gaactggtcg gcgggcgcgg caatattgcg acggtgagcc
    11941 actgtattac tcgcctgcgc tttgtcctca gccaaccggc caatgccaga ccgaaagaaa
    12001 ttgagcaact ccccatggtg aaaggctgtt tcaccaatgc cgggcaattt caggtggtga
    12061 ttggcaccaa cgtgggtgat tactatcaag cactgattgc gtcaaccgga caggcgcagg
    12121 ttgataaaga gcaggtaaaa aaagccgccc ggcagaatat gaaatggcat gagcagttga
    12181 tctctcattt cgcggagatc ttcttcccgt tgctgcccgc gttgattagt ggtggtttga
    12241 tcctcggttt tcgcaatgtg atcggcgatt tgcccatgag caacggtcag acgctggcgc
    12301 aaatgtaccc ttccctgcaa acgatctacg attttctgtg gttgatcggt gaagcgatct
    12361 tcttctacct gccggtcggg atttgctggt cagcggtgaa aaaaatgggc ggtacaccga
    12421 tcctcggtat cgtgcttggc gtaacgctgg tttctccaca gctgatgaac gcttatctgc
    12481 tcgggcagca gctgccggaa gtatgggact ttggcatgtt cagcatcgcc aaagtgggct
    12541 atcaggcgca ggtcatcccg gcactgttag ccgggctggc gctgggcgtt attgaaactc
    12601 gtcttaagcg catcgtgccg gattacctct atctggtggt ggtgcccgtc tgttcgctga
    12661 tcctcgcggt gttcctcgcc catgcgctga ttggtccgtt tggtcgcatg attggcgatg
    12721 gcgttgcctt tgcggtacgt cacctgatga ccggcagctt tgctccgatt ggcgcggcat
    12781 tgtttggctt cctgtacgcc ccgctggtga tcaccggcgt acaccagacc acgcttgcta
    12841 ttgatttgca gatgattcaa agcatgggtg gcacgccagt gtggccgctg attgcactgt
    12901 cgaatatcgc tcagggctcc gccgtgatag gtattatcat ttccagccgc aagcacaatg
    12961 aacgcgagat ctccgtgcct gccgctatct ccgcctggct tggggtcact gagcctgcta
    13021 tgtacggcat caacctgaaa tatcgcttcc cgatgctgtg cgcgatgatt ggttctggtc
    13081 tggcaggatt gctatgcggc ttgaacggcg ttatggcgaa tggcatcggc gtaggcggcc
    13141 tgccgggaat tctctcgatt caaccgagct actggcaggt gtttgcgctg gcaatggcta
    13201 tcgccatcat catcccgatt gtactcacct cgtttatcta tcagcggaaa taccgcctgg
    13261 gcacgctgga cattgtttaa ttttcttcgg ggcgcaattg cgccccctcg catttgcagg
    13321 aataacgtaa tgactcatct tccccactgg tggcaaaacg gcgttatcta ccagatttat
    13381 ccaaagagtt ttcaggacac cacgggtagc ggcaccggcg atttacgtgg cgttatccaa
    13441 cgcctggact atctgcataa actgggcgtt gatgccgtct ggctaacccc cttttatgtc
    13501 tctccccagg tcgataacgg ttacgacgta gcgaattata cggcgattga tcccacctat
    13561 ggcacgctgg acgattttga cgaactggtg acacaggcaa aatcgcgtgg gattcgtatc
    13621 attctcgata tggtgtttaa ccatacctct acccaacatg cctggtttcg cgaggcgctg
    13681 aacaaagaaa gcccttaccg ccagttttat atctggcgcg atggagaacc agaaacgcca
    13741 ccgaacaact ggcgttcaaa attcggtggt agtgcgtggc actggcatgc ggaaagcgaa
    13801 cagtactatt tgcatctctt tgcaccagaa caggcggatc tcaactggga gaatccagcg
    13861 gtacgcgcag agctgaaaaa agtctgtgag ttctgggccg atcgtggggt cgacgggttg
    13921 cgcctggatg tggtgaatct gatctccaaa gatccgcgtt accctgaaga cctggatggc
    13981 gacgggcgtc gcttctacac cgacgggcca cgagcacacg aatttttgca cgagatgaac
    14041 cgcgatgtgt ttacgccaca cgggttaatg accgtaggtg aaatgtcctc caccagcctt
    14101 gagcattgcc agcgatacgc ggcactgaca ggcagtgaat tgtcgatgac ctttaatttt
    14161 catcacctga aggtcgatta tcccgatggt gaaaaatgga cgctggctaa acctgacttt
    14221 gtggcgttga aaacattgtt ccgccactgg caacaaggaa tgcacaacgt ggcatggaat
    14281 gccttgttct ggtgtaacca cgatcagccg cgcattgttt ctcgctttgg tgatgaaggt
    14341 gaataccgcg tgcctgcggc aaaaatgctg gcgatggtgc tgcatggcat gcagggaacg
    14401 ccgtatatct accagggcga agagattggc atgaccaacc cacatttcac gcgcattact
    14461 gactatcgcg acgtggagag cctcaatatg tttgccgagc tgcgcaacga tgggcgtgat
    14521 gccgacgagt tattggcaat cctcgccagt aaatcccgtg acaacagtcg cacacccatg
    14581 caatggacca acggcgataa tgccgggttt acggctggcg aaccgtggat tggcctgggc
    14641 gataactatc aggaaatcaa cgtagaagca gcgctggccg atgagtcctc ggtgttttac
    14701 acctaccaaa agttaatcgc actgcgtaag caagaagccg tcctgacatg gggcgattac
    14761 caggatctgc tgccaaacag ccctgtattg tggtgctatc gccgtgagtg gaaggggcaa
    14821 accttgctgg tcattgccaa ccttagccgt gagacccaac cctggcagcc tgggaaaatg
    14881 ctcggcaact ggcagcttgt gatgcataac tacgaagaag cctcaccaca accctgtgcc
    14941 atgactttac ggccttttga ggctgtctgg tggttacaga agtaaatctt cgctaagccc
    15001 cggtaatgcc tgtcattccg gggttttgcc tgtcagtatg ttgtttttgt tgattttata
    15061 atcaaaaaat tcattaaaaa atttacatat cgctgtagcg cccgtcatcc gtacgctctg
    15121 ctttttactt tgagctacat caaaaaaagt tcaaacatcc ttgatgcaaa gcactatata
    15181 tagactttaa aatgcgtccc aacccaatat gttgtattaa tcgactataa ttgctactac
    15241 agctccccac gaaaaaggtg cgacgttgtg gataagcgga tggcgattgc ggaaagcacc
    15301 ggaaaacgaa acgaaaaaac cggaaaacgc ctttcccaat ttctgtggat aacctgttct
    15361 taaaaatatg gagcgatcat gacaccgcat gtgatgaaac gagacggctg caaagtgccg
    15421 tttaaatcag agcgcatcaa agaagcgatt ctgcgtgcag ctaaagcagc ggaagtcgat
    15481 gatgccgatt attgcgccac tgttgctgcg gttgtcagcg agcagatgca gggccgcaac
    15541 caggtggata tcaatgagat ccagaccgca gttgaaaacc agctgatgtc ggggccatac
    15601 aaacaactgg ctcgtgctta catcgagtac cgtcacgatc gcgacattga acgtgaaaaa
    15661 cgcggtcgcc tgaaccagga gatccgtggt ctggtcgagc agaccaactc ctcgttactc
    15721 aacgaaaacg ccaacaaaga cagcaaggtg attccaaccc agcgcgacct gctggccggg
    15781 atcgtggcta aacactatgc acgccagcac ctgctgccgc gtgacgtggt gcaggcacat
    15841 gaacgtggtg atattcacta tcacgatctc gattactcac cgttcttccc gatgttcaac
    15901 tgcatgttga tcgacctgaa aggcatgctg acccagggct ttaaaatggg gaacgccgag
    15961 attgaaccgc cgaaatcgat ctctacggca accgcggtaa ctgcgcagat tattgctcag
    16021 gtcgccagcc atatttatgg cggcaccacc attaaccgta tcgatgaagt gctggcaccg
    16081 tttgtcaccg ccagctacaa caaacatcgc aagaccgcag aagagtggaa catcccggac
    16141 gccgaaggct acgctaactc tcgtaccatc aaagagtgct acgatgcctt ccagtcgctg
    16201 gagtacgaag taaacaccct gcacaccgcc aacggtcaaa caccgtttgt gacctttggt
    16261 tttggtctgg gcaccagctg ggagtcgcgc ctgattcagg aatctatcct gcgtaaccgt
    16321 atcgccggcc tcggtaaaaa ccgtaaaacg gcggtgttcc cgaaactggt gtttgcgatt
    16381 cgcgatggcc tgaaccataa aaaaggcgat ccgaactacg acatcaaaca gctggcgctg
    16441 gagtgcgcaa gcaagcgcat gtatccggat atcctgaact acgatcaggt agtgaaagtc
    16501 accggttcgt ttaaaacccc gatgggctgc cgcagcttcc tcggcgtgtg ggaaaatgaa
    16561 aacggcgagc agatccacga tggtcgtaac aacctcggcg tgatcagcct gaacctgccg
    16621 cgtattgctc tggaagcaaa aggcgatgaa gccaccttct ggaagctgct ggatgaacgt
    16681 ctggtgttgg cacgtaaggc actgatgacc cgtatcgctc gtctcgaagg cgtgaaagcg
    16741 cgcgtagccc cgatcctcta tatggaaggc gcttgtggcg tgcgtctgaa tgctgacgac
    16801 gacgtttctg aaatcttcaa aaacggtcgt gcgtctattt cgctgggtta catcggcatc
    16861 cacgaaacca ttaacgcgct gttcggcggc gagcatgttt acgacaacga gcagctgcgc
    16921 gcgaaaggta ttgcgattgt tgagcgtctg cgtcaggcgg tggatcagtg gaaagaagaa
    16981 actggctacg gtttcagcct ctacagtacg ccgagtgaga acctgtgcga ccgcttctgc
    17041 cgtctcgata ccgctgagtt tggcgtggtg ccgggcgtga ccgataaagg ttactacacc
    17101 aacagtttcc acctcgatgt ggagaagaag gtgaacccgt acgacaagat cgactttgaa
    17161 gcgccttacc cgccactggc gaacggtggc ttcatttgct atggcgagta tccaaacatt
    17221 cagcacaacc tgaaggcgct ggaagatgtc tgggattaca gctatcagca tgtaccgtat
    17281 tacggcacca acacaccgat cgatgaatgc tacgagtgtg gctttaccgg cgagtttgag
    17341 tgcaccagca aaggcttcac ttgtccgaaa tgtggtaacc atgacacctc ccgtgtgtcg
    17401 gtgactcgcc gcgtgtgcgg atatttaggt agcccggatg cacgtccgtt taacgctggt
    17461 aagcaggaag aagttaagcg ccgcgttaaa catctgggaa atgggcagat aggttaatct
    17521 tcactctgtg ttaagcaaat gccggatgcg gcgtaaacgc cttatccggc ctacatttgg
    17581 ctacggctgt aggcctgata agacgcgaca gcgtcgcatc aggccttgat taccggatac
    17641 agcctacacg cggactgata agccaggtga aataatgaac tatcatcagt actatcctgt
    17701 cgacatcgtc aacggccccg gcactcgctg caccctgttt gtctctgggt gtgttcatga
    17761 atgccccggt tgctataaca aaagcacctg gcgggtaaat tccggtcagc catttaccaa
    17821 agcaatggaa gaccagatca ttaacgatct gaatgacact cgtatcaaac gccagggtat
    17881 ttccctctcc ggcggcgatc cgctacatcc gcaaaacgtg ccggatattc tgaagctggt
    17941 acaacgcatc cgcgccgagt gtccgggtaa agacatctgg atgtggacag gctataaact
    18001 cgacgaactc aacgctgcgc aaatgcaggt tgttgatttg attaacgtgc tggtcgacgg
    18061 caaatttgtg caggatttaa aagacccttc cctgatctgg cgcggcagca gcaatcaggt
    18121 ggtgcatcat ttgcgatgat aagttccctg ttacctgagt gcaggcaaca gggaaataaa
    18181 gaattaacga tacttctggt gataggcgtt gcgggtcgtt ttcagttgct ctgcggcagc
    18241 ctgcgcttct tttactttac cttcatttgc cagcttcagc gcgtcgtcaa tctgaccgac
    18301 cagaatgtcg aaaccgtggc ggaagtcttt catttccggg ctgtccggtg atttgccttc
    18361 aagcttcggc ggcgtcgctt tttgcgcatc cagtgccgca gcgcgcatct tcgttaacgc
    18421 gtctttgact tgcgccgcgt tatcggcttt ttcaaccact tttaaattgt cgttgagggt
    18481 ttccatattg tcttccagat cagcggcaaa cgacgccgaa ctgaatacca atgaggagac
    18541 tgcaagaata gctaacaggc ttttacgcat tgctcacttc cttttttatt atgaagattt
    18601 ggcgcggcag gtcgcgccag ttttttactg cccggcgatt ttcatctccg gcaacagcac
    18661 agaaccacac tgtatattac tgcgtgtttc aatatcgtta ccgacggtga caatattgcg
    18721 ccacatatct tttaaattac cagcgatggt gatttcgctc accggatact gaatctcgcc
    18781 attctctacc cagaagcccg ctgcaccacg ggaataatca ccggtaatgg cactcacgcc
    18841 ctggcccatc agctccgtca ccaccagccc ggtgcccatc tctttgagca tttgttcgaa
    18901 gcttagccct tgtccggcaa tccgccagtt gtgaataccg cccgcatgtc cggtgctttt
    18961 cagccccagt ttccgcgccg aatagctggt cagcagccac tgagtcagga tgccatcttt
    19021 gacgatatcg cggcgctcgg tgcgcacacc ttcgctgtcg aatggtgtag atgccagccc
    19081 tttcagcaga tgcggatgct cttcaatggt cagccaatcc ggcagaattt gtttacccag
    19141 cgaatccagc aagaaggtcg atttacggta gaccgcacca cccgctatcg cccccaccaa
    19201 atgaccaaac aaccctgttg ccacttcatt ggcaaaaatc accggcgctt tcatggtaga
    19261 aagtttacgc ggtgacaggc gcgataaggt acggcgagca cagtcggccc caacccactc
    19321 tggcgtttgt agatcgctca tcgcacgccc aatggtgtag gcgtaatcac gctccatatc
    19381 gccattttct tcggcgatta cacagctgga gagcgaatga cgcgtagagc agtacccctg
    19441 caacatgccg tggctgttgc caaaaacttt gacaccgtag tggctgttaa agctaccgcc
    19501 ttcggtattg gtgatgcgtt tgtccgcctg caatgccgcc tgttccgcgc gagccgccag
    19561 ttcaatggct tcatccggag aaacgtccgc agggtggaac aagtcaagat ccggtgcgtc
    19621 aaaggccagc agttctttgt ctgccacgcc ggcatacgga tctggcgagg tataacgagc
    19681 aatatccagc gccgcctgta cggtgcgggc aatggcctgc gggcttaaat cggtagacga
    19741 tgcgctacct ttacggttct ggtgataaac ggtgatcccc agcgcgccat cgctattgaa
    19801 ttcaacattc tccacttcac cataacgcgt gcttacacta atgccggtgg tcttgctgac
    19861 ggcaacttct gcaccgtccg atttgcctga ggccaactcc agcgcagtcg aaactgcttc
    19921 ttccagaatc ttgcgctgcg cttcaacttg agagattact ttcattgcaa gtgccataat
    19981 gtagagagtt tctctgaagt ctaacagaga actgtttttc agtgcgcatc ttaactggta
    20041 acattagcct ctttttttaa ggagcctgag atgactaagc agcccgaaga ctggctcgac
    20101 gacgttcccg gtgatgacat cgaagacgaa gacgatgaaa ttatctgggt cagtaaaagt
    20161 gaaattaaac gtgatgccga ggagctaaaa cgccttggcg cggaaattgt tgatctgggg
    20221 aaaaacgcgc tggataagat cccgttagat gcggatctgc gcgcggctat tgagctcgcc
    20281 cagcgtatta agatggaagg tcgccgccgc cagttgcagc tcatcggtaa gatgctgcgc
    20341 cagcgcgatg ttgaaccgat ccgtcaggcg ctggataagc tgaaaaaccg tcacaaccag
    20401 caggtggtgc tgttccataa actggaaaac ctgcgcgatc gtctgatcga ccagggtgat
    20461 gacgccatcg ctgaagtatt gaatctgtgg cccgatgccg atcgtcagca actgcgtact
    20521 ctgatccgta acgcgaagaa agagaaagaa gggaataagc cgccgaaatc cgcacgccag
    20581 attttccagt atctgcgcga gttagcggag aacgaagggt aattttcgtt gttgatgtga
    20641 gtatggtttg ccggatacgg tgcacctgta ggcctgataa gacgcatcaa gcgtcgcatc
    20701 aggcactatg ctcaaatgcc ggatgcggcg tgaacgcctt atccggccta cacacactct
    20761 atcaggctga ggctgtgaat tactgcgcgg cttccgcttt cttcgctaac ccatccagca
    20821 gtttctgatg gatcccacca aaaccgccgt tgctcatcac cagaatgtga tcacccggct
    20881 gagcggtttt caccaccata tctgccagcg tatccacatc gccgctccag tgtgcaggct
    20941 gaacgcaggc ttctgccact tctgccacct gccacggaat atgtgccggt tgcagcagga
    21001 agacttcatc ggcacgacct aatgaaggtg ccagatcgtc tttgcagatc cccattttca
    21061 tggtattaga gcgcggttcc agtacggcaa taatgcgcgc cgtaccacca actttgccac
    21121 gcagcgccgc cagcgttgcc agaatcgccg tcgggtgatg ggcaaaatca tcatataccg
    21181 tgacgccatt cgcttcacca cgcaactcca gacggcgacg agcgttaata aacgaaccca
    21241 gcgcgttagc ggcatctgcc ggcgctacac caacatggcg agccgccgca atcgccatca
    21301 ggccattgtg catattatgt tcgcctacca gcgaccattt cacttcgccc actttttcgc
    21361 catccagcaa gacttcccat tcagaagcgt cagtggtcag ctttttcgcc tgccagtgac
    21421 cttgctcgcc caccagctcc tgctcgctcc aacagcccat cgccatggtc tgtttcaggt
    21481 tgatgtcgtt ttccggccag ataatacggc cctgccccgg aacgatacgc accagatggt
    21541 ggaactgttt ctggatcgct ttcaggtcgt caaagatatc ggcgtgatcg aactcaaggt
    21601 tgttgaggat cagcgtacgc gggcagtaat ggacaaattt agagcgtttg tcgaagaagg
    21661 cgcagtcgta ctcatccgct tcgataacaa agaagtcgct ttcgcccaga cgcgccgaaa
    21721 cctcaaagtt ccccggcaca ccgccaatca caaatcccgg tttgtaaccg cattgttcca
    21781 gaatccaggt cgccattccc gccgtggtgg ttttgccgtg tgtaccggca acggccagca
    21841 cccagcggtc gcgcagcaca aaatcgtgca gccactgcgg acctgacata taagggatgt
    21901 ttttttccag taccgcttcc acacacggat ttccacgggt catggcgtta ccaataatca
    21961 ccagatccgg ctgtggatcg agctggctgg catcgtaacc ctgaatcagt tcaatgcctt
    22021 gcttctcaag taaggtgctc atcggcggat acacattggc gtccgaaccc gttacttcat
    22081 ggcctaactg gcgcgccagc atcgccagac cgcccataaa cgtgccacaa attcctaaaa
    22141 tatgaatgcg catacgtcac tatccttttt taatctggcg ctcattttac gcatatgtcg
    22201 ggtaagtgag aaatgcattt caggataatc cgtaatttgc tggcgcgatt cacctgagcg
    22261 caacattggg attatttgtt aagattgttg cggtcgcttt actccataaa cattgcaggg
    22321 aaagttttat gaaaacgtta ggtgaattta ttgtcgaaaa gcagcacgag ttttctcatg
    22381 ctaccggtga gctcactgct ttgctgtcgg caataaaact gggcgccaag attatccatc
    22441 gcgatatcaa caaagcagga ctggttgata tcctgggtgc cagcggtgct gagaacgtgc
    22501 agggcgaggt tcagcagaaa ctcgacctgt tcgctaatga gaaactgaaa gccgcactga
    22561 aagcacgcga tatcgttgcg ggcattgcct ctgaagaaga agatgagatt gtcgtctttg
    22621 aaggctgtga acacgcaaaa tacgtggtgc tgatggaccc cctggatggc tcgtccaaca
    22681 tcgatgttaa cgtctctgtc ggtaccattt tctccatcta ccgccgcgtt acgcctgttg
    22741 gcacgccggt aacggaagaa gatttcctcc agcctggtaa caaacaggtt gcggcaggtt
    22801 acgtggtata cggctcctct actatgctgg tttacaccac cggatgcggt gttcacgcct
    22861 ttacttatga tccttcgctg ggtgttttct gcctgtgcca ggaacggatg cgcttcccgg
    22921 agaaaggcaa aacctactcc atcaacgaag gaaactacat taagtttccg aacggggtga
    22981 agaagtacat taaattctgc caggaagaag ataaatccac caaccgcccg tatacctcac
    23041 gttatatcgg ttcactggtc gcggatttcc accgtaacct gctgaaaggc ggtatttatc
    23101 tctacccaag caccgccagc cacccggacg gtaaactacg tttactgtat gagtgcaacc
    23161 cgatggcatt cctggcggaa caagcgggcg gtaaagcgag cgatggcaaa gagcgtattc
    23221 tggatattat cccggaaacc ctgcaccagc gccgttcgtt ctttgtcggc aacgaccata
    23281 tggttgaaga tgtcgaacgc tttatccgtg agttcccgga cgcgtaatct cctgcgcctc
    23341 tccacacgga gaggcgtttt tatcgctgca caatgttgac tcttgtcaca ggcgaactct
    23401 gacgattctc ccacagcacc gttaatccac gctgtaatgc tataaaaata aacaacaaaa
    23461 taccgatggc gattttcgtc caccaggaac tcagcgtgcc atcaaagttt atgtaagtct
    23521 gaatcagtcc ctgaatcgcc acgccaaaaa gcgtccctaa taccgttcca acgccaccac
    23581 tcaaaagcgt accgccaatt accactgagg cgatagcatc cagttccaca cctacgcccg
    23641 ccagtgcata tccggcctgg gtataaatcg agaagacaat ccccgccagc gttgccagtc
    23701 cggtggagag catataaatg cgaatagtgg tgctgcgagt ggaaatcccc atcaggtttg
    23761 ccgacgttgc gttgccgcca atggcgtata cctgattacc aaaacgggta cgatgcgcga
    23821 ggaatatgcc gataaccacc accgccagca tcagcagtcc catcgcactt aagcgaccgc
    23881 cgccagggat tttccacgca aggcttgaga gcgtgtcata aacaggatgg tttatcggga
    23941 tcgactcttc cgaaacgaga tagctgacgc cacgcaaaaa gaacattccc gcaagggtaa
    24001 tgataaacgc cgggatcttc agggcgtcaa tcagaagccc cataaatgcg ccgaaggcac
    24061 agcccatcac caacaccagc ggaaacgcca gcagcggcga gaggccgaaa tcgccaatca
    24121 cttttgccag aaacacgccg gtaaaggcga tcaccgaacc gacggagaga tcgatcccac
    24181 cggagaggat cacaaaggtc atgccaacgg cgattatccc aagaaaggcg ttatcggtca
    24241 ggatattgca gatcactctt gtggaagcaa aaccgggaaa ctgggtcaga cagtagatat
    24301 aacccaacac aaagacgccg atggtgatca tcagtggcaa attacgtttt atcacggtta
    24361 cgtacccctt taatcagact gataaagcgt tgcgactgaa caatcagcac gcaaagcacc
    24421 accaccgctt tcaccacctg gttcatctcc ggcggaaagc cagaaagcaa aattccggtg
    24481 ttcattccct gaataatcag cgcccccacc accgaaagca gcaggttaaa gcgcccgccc
    24541 atcagcgatc cgccgccaat caccactgca agaatggcgt ccagctccag ccataacccg
    24601 gcgttattgg catcggcacc gcgaatatcc gccgccacga taatgcccgc aatcgccgca
    24661 catagcccgc tcaacacgta ggtgagcatg acgatgatcc gcgtgtttac cccggcattt
    24721 tttgccgccc gaatgttgat accaacggct tcgataaaca tccccagcgc cgttttgcgg
    24781 gtcaacagcc agaacaggag aagtgtcagc accgcaataa tgactggtgt tggcaggaac
    24841 aacagcgatc cactgccgaa ccaggagaga tccggtgagt taaatgtgac gatctgcccg
    24901 gaggtgatca gttgcgccac gccgcgcccg gcgaccatca ggatcagggt ggcgacaaac
    24961 ggctgaattt tgaggatcgc taccagtatg ccgttccaca atcccgccag gatgccagtg
    25021 ccgagggcgc ttaacaaaac aattggaagg ctgaatcccg cgaccgtcat cgcagccgtt
    25081 gtggctccgg cgatagccat caccgcccct acggagagat caatcccacc agtggcgata
    25141 accagcgtca tgccaatcgc cagtagcgca acgggggccg cacggttaag aatgtcgata
    25201 gggctaccga acaaacgccc atcctgaagc accacctgcc agaaatgcgg ggccaccagg
    25261 ctatcgacca gcagcaccag caacagtgcc gccagctgcg gcattcctgt tggccagcga
    25321 aaacgccttt tcggcggcgt agtgtccggg agagattgag gcatcacact gttctcctta
    25381 cgccgcaatg gcgttcatga tcgccggaac ggaaagctct gccagcggga tctccgccac
    25441 ctgtttgcga tcgcgcatga tgatcacccg atcggcatag cccaccagct cttccagctc
    25501 ggaggagatc accagcagcg ccagcccatc ggcgcacagc gtttcaatca gccggatgat
    25561 ctcggcgtgg gcaccaacat caattccgcg cgttggctcg tcgagaatca gaaattgcgg
    25621 tcgggtcagt agccaacgtg aaagcaacac tttttgctga ttgccgccgg agagaaattc
    25681 aatcggttgt tcggtggaag gtgtgcgaat gccaagctgg cggataaagc gttcggcaat
    25741 ctcttgctgc tctttgcggg aaataggtcg tagccagccg cgctgggcct ggagagcaag
    25801 aatgatattt tcccgcaccg aggcggcggc gatgatgcca tcggttttcc tgtcttccgg
    25861 gcagaagccg atgcccagta ccgaagcctg atgtggcgag cgtggcgttt gtggtttgcc
    25921 tttgatcaac gcagtgccgc tgtcagcagg tttgataccg aagatcactt cggcggtttc
    25981 tgtccgccct gatcccagca agcctgccag accgacgatc tcgccggggc gcacttcgag
    26041 atcaaacggt gcgatcgttc cttttttgcc gtaatttttg aacgcggcaa caggtttgtc
    26101 gctcagcaat gttctcccgg cacgctgaag cgcgtgggtg tccagctcgc gccccagcat
    26161 catttttacc agttcgatct gcggtagctc gcgcgtttcc cgacagccca caaaactgcc
    26221 gttacgtaag acggtgatcc gatcgctaac ctgataaacc tgatcgagaa aatgagtgac
    26281 gaagatcagg ctgacgccgc gatcgcgaag ctgacgcatc aggccaaaca gtaactctac
    26341 ttcctgagtg tcgagactgg cggtgggttc atcgaggatc agcactttgg cggagagatc
    26401 gatagcccgg caaatggcga cgatttgctg catcgcgact gaaaagcggt tgagcggttc
    26461 gcgcacgtcg agggagaaac cgtaagatgc catcagttcg gtggcgcgct tttccatctc
    26521 tttacggcgt agaaggccga aacgtttggg ttcgcgacct atgaatagat tatcagcgac
    26581 cgacatattg ggtagcaggt tgacttcctg atagacggtg ccgatgccga gttgttgtgc
    26641 gtgggcggtg tttttcggtg agatagcctg gccttccagc cagatagtgc cgcgatcggc
    26701 gtggtataca ccggttaatg ctttgattag cgtcgatttt cccgccccgt tttcaccgag
    26761 cagcgccatg atttcgccac ggcgcaggct gaaattaacg ttgtctaacg ctttgacacc
    26821 ggggaaaaat ttacttaatc cttcggtgcg gaggatttcc tggtgttggt cggtcgtcat
    26881 ggtttccccc ttagccacgc tctaaaagtc cgggaataga tcgtgcctga tgttaagagc
    26941 tgcgccgttt gtccccctca ccctaaccct ctccccaaag gggcgagggg acagattgtg
    27001 catggtgtta agtccggtac tgcacaactg ccggatgcgg cgtaaacacc ttatccggcg
    27061 gcccacaagt cggcatcaga tattgatgcc cctcaatacc ccatgttttt cttcttctct
    27121 aactcttctt ttgcggtatc aggcaggtaa agggtggact tggttaacgt cagcttttca
    27181 ggcatggtgc cgtctttttt gtatttctcc agcgcgtcaa aagcggggcc agccatgttc
    27241 ggcgtcagtt caacgctggc gtttgcttcg ccatcaatca tcgctttata gatatctggt
    27301 acgccatcaa tggaacccgt gaggatatct ttacccggct tcaggcccgc ttctttaatt
    27361 gcctgaattg caccaatcac catgtcgtcg ttatgggcgt aaaccatgca gatgtttttg
    27421 ccgttgtttt ccgctttgat aaagctctcc atgacttctt tgcctttact gcgggtgaag
    27481 tcacctgact gcgagcggat aattttgata tttggcgcat tctttatggc ttcggcaaag
    27541 cctttcttac ggtcaatggc gacgctggcc ccaacggtgc cctgcagctc caccacgtta
    27601 catggtttgc cgttaacctc tttcaccagc cagtcaccga tcaacttacc ttcgaggatg
    27661 ttgtcggcag taacggtggt catatagaga gatttgtctt tcacatcgat ggaacggtca
    27721 agcaagaaga ccgggatttc ggcatctttc gcctctttta ataccggttc ccaacccgtc
    27781 gcaaccaccg gagcgataaa gatcgcatcc accccttgtg cgacgaagga acgtaccgct
    27841 ttaatctggt tttcctgctt ttgctgacca tcggcaattt tcagtgtgat accgcgcttt
    27901 tcggcttcac ttttcgccac attggtttct gcggcgcgcc agccggattc cgatccgacc
    27961 tgcgaaaatc ctacggttaa tggagcggcc aacgccatag acgacatggc tgccgagact
    28021 gcagagacta caagtaagcg tttccacata gggttgtcct cgtcggggta attattattg
    28081 gtgaaaagat gttcgcgaaa aaactataga caattcgtta tgtaacggat tgcgttacat
    28141 aacacttcaa aaaagtaaat aaaacgttaa tcacaagttt gtaatcgctt tcatctcact
    28201 atgaaaaatg cggctacggt tatggatttt cctgctctgt ataccgtctt aaaactggcg
    28261 aaaaaggaaa atgaagacga aaacaagcga agacattcgg cgcgagttgg ctataatact
    28321 cggcacttgt ttgccacata tttttaaagg aaacagacat gagcttactc aacgtccctg
    28381 cgggtaaaga tctgccggaa gacatctacg ttgttattga gatcccggct aacgcagatc
    28441 cgatcaaata cgaaatcgac aaagagagcg gcgcactgtt cgttgaccgc ttcatgtcca
    28501 ccgcgatgtt ctatccgtgc aactacggtt acatcaacca cactctgtct ctggacggtg
    28561 acccggttga cgtactggtc ccgactccgt acccgctgca gccgggttct gtgatccgct
    28621 gccgtccggt tggcgtactg aaaatgaccg acgaagccgg tgaagatgcg aaactgattg
    28681 ctgttccgca caccaagctg agcaaagaat acgatcacat taaagacgtt aacgatctgc
    28741 cagagttgct gaaagcgcaa atcgctcact tcttcgagca ctacaaagac ctcgaaaaag
    28801 gcaagtgggt taaagttgaa ggttgggaaa acgcagaagc cgctaaagct gaaatcgttg
    28861 cttccttcga gcgcgccaag aataaataag ttcttctggc gcaataaccc tgaacaccgg
    28921 gcttcggtta gtaagggttt ttttatgccc gcaatttata aactctctac tcctattatt
    28981 attttcagca gttgaaaggc ttgaacggta agaacaagct aagctcgacc accattttgc
    29041 tgttcatagc cacttgctgg aagttagccg gcctcactca tactcaccgt tcaaagcgtg
    29101 tccccgtttt acccctgtac atttgttaac gccagcagag ttttttatgc gattgcagat
    29161 atggaaagcg gcccggagag aaaagcaaaa gatgggaaag tgacaaaaaa aatcgcgaga
    29221 tacatcgcat aaaacaacgc cacccgaagg tggcgtgctt ttattattac ttatccctgt
    29281 ctaaccagtc gccgctttca attagcttta atccatcgac gggtcgttga taaacgtaca
    29341 tccatgcact cccgtacggc gtctgaatca actggcgcgc gtattcaccg cccctggtgc
    29401 gcaaggcatc aagttcggcc agcgtggcgt tgtcaatacg ataaacttca ccgtgtactg
    29461 ttccgttccc cggaactgcg cctggatagt ggcccaggct atacaactgg tagttatcga
    29521 tactgaaatc gcccagtaac tgggcattgg tcatccagtg actgttgcct tgtttgtggc
    29581 gtaaactgcc gtagacaaat attcgcattg ctaaaactcg aactgataga gcaaatccag
    29641 tgcctggtct acaccagaca cggcttccag atatagctta ggcatcaggc gataacgtaa
    29701 cgtgagtgtt gctatagagt caaatatacc cacgccgtat ttcacttgca gacctggcaa
    29761 tacatagccg ctgaccacca cctgggagga gtcgcctact ccctctgtgt cgagcgctaa
    29821 attgcttacg ccaaacgtct cgccgatttt acccacaatc tggccacttt gcgcaacccc
    29881 caggccaatc agcatcgagg tcatcgccgc actgtcactc tgatcgctct ccagcccttg
    29941 tccacgtagc aaataagaca atgcagcttg ttgcgacatc gccgggtcag agaagatctc
    30001 cgctttcggt tcgtccgcca gaccagtgac gcgaactccg gcgatgacgt cgtcttctgt
    30061 agcatccggg ttacgaatag cttcgatatt aagatacggt tgatctggcg gaccagagaa
    30121 cagtaactca cccttacgca caatcagatc ctgaccatag gcatggaagc gcccttcagg
    30181 gatgttgatc tgcccgttca ggcccagccc ttgtttgtcc tgaacgacgt tgagatcgcc
    30241 cgtcagccgc gctttcaggc caaaggcgtc aatgcgcaca ttgttgccaa cgtggacagt
    30301 caggttactg ttaatcggaa tcgacgccgt tttcggctct tccggttgca ggttatcgtt
    30361 aagcatcacc acatcgctgg aaacgcctac tgcgctttcc ggcagctcgt gcaccacgat
    30421 gcgcgcccaa gggacatcca cgcgaccgtc gagagtaaac agatttggtg tagcctcgaa
    30481 tacaacatct ggcgatacat ccattcgtac catcggcggc acggtgatac gcactttact
    30541 gcccttcgct gttaccctcg cccgccagtt ttcaatctgg ctccagtcgg cgtcaccgct
    30601 caggtagatt tctccctgtt gggtccgcac agtaccagca agcgtcgagc gcataccgtt
    30661 aaagttgacc gcaagctggc tcggttgcat atcaaacggc ataaagttgc cgtcgatatc
    30721 cacccccgta acctgaagct gaccaaacaa ctgcgggctt tgcacatcac cacccagacg
    30781 caagttggca ctcaccatcc ccgctgcttt ttccccacgg gtaaagatgg ggtttatcat
    30841 cgcaaggttg aagttacgga tattgacgtt gccaccaaga ttacggcggc cttgcggatc
    30901 ggtcacctgc acctgaccat caaactggcc gttattggtc aggcggatgg tccagcccag
    30961 ttcggcacgg ttgttacgca gttccgccgt cagattcagc gtctgaaacg ccacgggcag
    31021 cgccgcatcg ttgacggttt gcgtcacctg cacattacgc cccgaaaggg tgatactgcc
    31081 ctgcggcagc ccctctttcg tggtgtccca ggcaacgtcc gctttacccg tgaagatacc
    31141 gctggcctga gtggtttctg gcataaatgg tttcagcatg gccaggtcga agcggttgag
    31201 attcaccacc gcacgccctt cggcacccgc atcgatagtt tgcggcacgc acagttccgc
    31261 attcgggtta agccagcagt gtggcccgat gctgattttt tgttccttat tgcggtaatc
    31321 cagcgcaata tcgcgggtca gcgaccacgg accgaccggc gtctggaagc gcgtattgct
    31381 aagtgttccc ttccagcgtt cttctttgcg atcaaaactt cctgccagat taagctgccc
    31441 ggaaatcggt tcgccctgaa tccgcaactg tagctcgtgc tgcttttcgc tgcctttggc
    31501 attcagggtg acgaggttga tatttacatc cggctgcgag atttgctcaa cgcgtacgtc
    31561 gagtttcccg gcgatctgat cggtggattt aatgtcgcct tccacgcgaa cctgcgccac
    31621 ggaaagttcc tgccagcgca ggccgcgcgc ggtgatatct gccagtagtt gtggcgcttc
    31681 caccgtgccg cgtactttca ccagcccttt cgctgtaccg ccaagccccg gcagcgcgtt
    31741 atccagcccc ggcgcgttga tggtggcatc aagattgaga tcttttaccc ccagctcacc
    31801 tttcacttcg gcactgtttg gccccagttc cagatgcagc cctgggatca tccactgcat
    31861 ataactgttg cctttcagcg tgccgtcaac gttcactttg ttctgtttga cgttaccggt
    31921 cagcttcagt tctggcacct ccatctgcca ggtgccgccg tacaggctac cgcgggtttt
    31981 aatcaagcca ttgagtttcg acggccactc cgggatctct ttggcggtgt taatgccgtt
    32041 aagcgttagc tcaccgcgcc aactaatggc ctgctgccag tcgagcaacg ccttaagttc
    32101 cgttttccct tccagcgccg cgacggtaag tttgtcgaga ttgacctgct gttcattacc
    32161 tttggcatca agggtaatgg tggctggcgg gatctcctgg cccttaacag ccgtgcgcat
    32221 agagatcgcg taatcggtca ttttgccggt aagtttcagt ttcagatcgt ctgcctgata
    32281 ctgcttctca ccagtgaacg gccagtaaag ctgtttgctg ttcacttcca cgttgagcgg
    32341 caatccggct tccgccagtc gcgtctgggc gcgtaaatcc atatccaccg gaccggaaag
    32401 gttaacgcct atctccagct gttcgcgcag cgcaccgccc actttcagct tcaccttttc
    32461 acctttcaac ggctccacgt tcagtgtgct gttgagggtg atatccaccg gccagttgtc
    32521 tgacagctgc gccgtgccgc tggcgttgac gatcccttga ctggagtcga tatccagggc
    32581 gtccagttta gtattgccgt caatgctgct cactttcagc agcatggtgc tcacggtgat
    32641 gtccgtgtcg cccgtcacgc gcagctgctc gcccttaaac tcttcaatgt tcaggttaag
    32701 cggcaaatgc acgtcggtca tttccggcaa taccgggcga gaaaagagat ctttcagcgt
    32761 ttcgccgagc ggcttgtcct ccggctgcgg attttcaatt ttcggttcga ccacttcttc
    32821 ctgtgccgct tccgccactt tcggcaaagc aatcaacagg cctatcagcg atgtcggttt
    32881 cagggtcagg gttttctcct gccagttcag gccggaggtg aagtccatca ccgataccgt
    32941 ggtgtcatca atcttgatgt tgacgttgtc cagcgccacc cgtgtcaggg taatgggata
    33001 cggcgtggag agatccaacg gaccgctatc ttcctcttct tcaacctgtt cagaaggagg
    33061 cattttttta ctgtcgatgt tgacctgaat gtctttcagc gccagatcat taatacaaac
    33121 gctactgttc cacaggcact caagcccgac cgccagatgc agattgcccg cttttaccgc
    33181 cacgcctggc tgctcataac gaacgtcaga caaggtgaga tcacgccagc cgccggtgac
    33241 cttgccaata tccagtcctg gcacccagcg atccgccgct ttaaatacca gatgcaggcc
    33301 gctggtggtg cccaccagaa acgccaccga tcccagtaac agtaagataa cgataaccac
    33361 gccgaggctg atttttttcc ataaactcat aattctggcc ccagaccgat gtaaaactgt
    33421 aacccgtgtt cgtctttatc cgcgaccggc acggcaaaat cgagtttgat tggcccgacc
    33481 ggcgattccc agcgcacgcc gacccctgta ccggttttaa agtcgctgcg gcgaatatcg
    33541 cttaccgctt cgccgctatc gacaaacacc gcgccccacc attttccggt cacgttgtac
    33601 tggtattcca gcgatccggt tatcagcttc gaggcccctt tcaggtcacc gttggcgtat
    33661 ttcggggcga tagatttgta tttgtagccg cgaatactgc gatcgccccc ggcgaagaaa
    33721 cgcagatccg gcggtacttt gtcgaaatca cccgtttcaa tccagcccag cgtgccgcgt
    33781 gtcacaaaac gatggcgatc gtacagtgtg cggatccaga cgttctgcgc ctggaaaacg
    33841 gagaaatcaa catccgagcc ccaggccgtg ttggagtagt cgatagagta gcgttgcgag
    33901 tcgccccagg ttggcatcag gccaccacga gaacgcgtgc ggctaatcat caccccagga
    33961 taaaacagca tcgtggtgtt ggtaatttca ccctgggtaa agtggtcgaa actccagcgc
    34021 aggttaatgg cacgctgcca gccgctggag agatcccagt agcgagaagc caccagcgta
    34081 gtggagtcag actcggtatc gttcaggtca gtgcgcttaa aaccgccctg caccaaataa
    34141 tattgttcca gcggattctt cagcagcggc attttatagc tgaagtcgag gatctgttcc
    34201 ggcgcggaaa tactggtact ggtggtcaaa ctgtgaccgt aagagttcat ccacggcttt
    34261 ttccacgtcg ctttcacgcg cggtcccacg tccgtagagt aaccgacgcc ggtttcgatg
    34321 gtgttttctg ttcgcggcga aaccacgccc gtcaatggta atactttcgt ttcgcgcgct
    34381 ttatcaaatt gtggagccac caccaccgag ttaaaccagc cggtagcaga aagtcgacgg
    34441 ttcagttctg ccagatcttt cgattcgtac tcatcgccct ctttaaacgg caccagattt
    34501 tgcaggtatt catcgcggat ttgtgatcct tcaaaggtca catgcccaaa gcggtaacgt
    34561 tcgccactgt tataatcaat atcccagaag gctttatgca ggccgagcgc aatgcccagc
    34621 tgtgctttgg taaattcgct atcgaaataa cctttacgca acgcaatgct ggttaaggac
    34681 tttttgaaat tttcataatc gccctggttc agtaccgtac caatagccgg gcgagtatcg
    34741 agcaatttca aatagtcttt atcggtccgc gcgccgccgc gcaataccac atcggtgccg
    34801 ccaattaaca ccggcacgcc tggcgtgact ttggcgatca atacctgccg ccctttcttt
    34861 ggcggtggac ggagatcaaa ttcaatggtc ggctggtaat aacccagcgc tttcagacct
    34921 tcgcggatgg catcatcgac gcgtgcgcga aagcgacggt ctggcgtcac ttcatcactt
    34981 tcaatcgtag aaagctgcgc gcgaacgttc ttttccagct gtcccgataa cccctcgacc
    35041 tgtagacgga cgttcgcggc gacggcagat ccgcttaagc agagtaagct tacacagcat
    35101 aactgtcgga tatagcgcac attttctcct gaatatcctt tttttcctgc ccctggaacg
    35161 ccgttaaacg gcttaacaaa aatccagtaa tatggattaa aaagcagact aaacccaaaa
    35221 tatttcttat gttttacttt agacctattc acggtggtta ttgtgtgcaa atacgcctct
    35281 tgttacaacc ttaaccccaa tgaccgattt tcgggagagc gacaccatga gtttatttga
    35341 taaaaagcat ctggtttccc ccgccgatgc cctgcctgga cgtaacaccc cgatgcccgt
    35401 agccacactg catgcggtca acggtcactc aatgaccaat gtacctgacg gaatggagat
    35461 tgccattttt gcgatgggtt gtttctgggg tgtggagcgt ctgttctggc agttacccgg
    35521 cgtttacagc accgccgcag gctataccgg cggctatacg ccaaatccga cttatcggga
    35581 agtgtgctcc ggcgatactg gccatgccga agcggtgcgc attgtttacg atccatccgt
    35641 catcagctat gagcagttgc tacaagtatt ttgggagaat cacgatcccg cccagggaat
    35701 gcgtcagggc aatgaccacg gcacgcagta tcgttcagcg atttatccgc tgaccccaga
    35761 acaggatgcc gccgctcgcg ccagtctgga acgttttcag gcggcgatgc ttgccgccga
    35821 tgacgatcgt cgcatcacca cggaaatcgc taacgccaca ccgttttatt atgccgaaga
    35881 tgaccaccag caatatctgc ataaaaaccc gtatggttac tgtggaattg gcggaattgg
    35941 cgtctgtctg ccgccggaag catagcgtta cgggtacaaa tgtagattgt tgataaagtg
    36001 cattttgttt atgccggata cggcgtaaac gccttgccag gcctacaaaa tcgtccaaat
    36061 tcaatatatt gcggggactg cgtaggcctg ttcggcatag cgcatcaggc agtttttcat
    36121 ttttcagctg tctgaatttt aaccctctgg cggctttaca gtaccttacg ctatactagc
    36181 cactgaaaat gccggttcac tttcttcgaa tcggctttca atgtgtattt cacacaaatt
    36241 aatcaacttc cccttccgag gatctggcct gaaaggtcgg ataagatatg ttaaacagta
    36301 ttttagtcat actctgtttg atcgctgtaa gtgcgttctt ctcgatgtcc gagatctcgc
    36361 ttgccgcctc acgcaaaatc aaacttaaac tgctggctga tgaaggcaat ataaatgccc
    36421 aacgcgttct gaatatgcag gaaaatcccg gcatgttctt taccgtggtc caaatcggtc
    36481 tgaacgcagt ggcgattctc ggcggtatcg tcggtgatgc ggcattttct ccagcttttc
    36541 acagcctgtt ctcccgctat atgtcggcag aactctctga gcaactgagc tttattctct
    36601 ctttctcgtt agtgactggc atgtttatcc tgtttgcgga tttaaccccg aaacgcatcg
    36661 gtatgattgc gccagaagct gtggctttgc gtatcatcaa cccgatgcgc ttctgcctgt
    36721 acgtttgcac cccgctggtg tggttcttca acggactggc gaacataatc ttccgtattt
    36781 tcaaactgcc aatggtacgt aaagatgaca tcacttctga tgacatctac gcggtagtgg
    36841 aagccggtgc gctggcgggc gtgttacgta aacaggaaca cgagctgatt gaaaacgtct
    36901 ttgaactgga atcccgtacc gttccgtctt caatgacacc gcgtgaaaac gtgatttggt
    36961 ttgatctcca cgaagatgag caaagtctga agaataaggt ggcggaacat ccgcactcta
    37021 agtttctcgt ctgtaatgaa gatattgacc acatcatcgg ctatgtcgat tctaaagacc
    37081 tgctgaaccg cgtgctggct aaccaaagcc tggcactgaa cagcggcgta caaattcgca
    37141 atacgctgat tgtgccggat acgttaaccc tttcagaggc gttggaaagt tttaaaaccg
    37201 caggtgaaga cttcgcggtg atcatgaacg agtacgcact ggtagtgggg atcatcaccc
    37261 tcaacgacgt gatgaccacg ctgatgggcg atctggtcgg tcaggggctg gaagagcaga
    37321 ttgtcgcccg tgatgagaac tcatggctga tagacggcgg cactccgatt gacgacgtca
    37381 tgcgcgtgct ggatattgac gagttcccgc agtcgggcaa ctacgaaacc atcggcggct
    37441 ttatgatgtt tatgctgcgt aagatcccga aacgcaccga ttcggtgaaa ttcgccggct
    37501 acaaatttga agtggtggat atcgataact accgcatcga ccagctgctg gtgacccgaa
    37561 tcgacagcaa agccaccgcc ctttcgccaa aaatgcctga cgctaaagat aaagaagaaa
    37621 gcgtcgcata accccagaaa catcaacggc tcctgaatca ggagccgtct tattactgca
    37681 tagcactttg gttaagccat ctctgtttgc agacgcataa cctgacggtt aacttcggac
    37741 atcactgaca aatgcagctt atccttcact tttgggataa gaatcttacc cttatcaaat
    37801 tcaaaagcgc caacgtcctt gatgtataac cgtccacgga acaggatctt tacgtacttc
    37861 gccacctgaa gtgggttgta ccgttggaaa attttcattg ttatctcctg ctgagtatta
    37921 cgcctttgcg gtgccacaat cggcccaact attatgaggc gcaaatttta atgcctagtg
    37981 actatagact atccgggcaa tgtttccacc gcgtataact ttttttacct aaaggttaca
    38041 attattcaga attatctttt taccgaagcg cggtcttcag tataagcatt catttttaat
    38101 atgatttgtg cgcttgaccg caaactggca tcacacttgc gggaaattcg ataaatagca
    38161 catatgatta aaactcagac ccaagtggtc ggatcacctg catatcataa gaaggaaaca
    38221 ccatgaccct acgcaagatt ctggcactca cctgcctgct gttgccgatg atggcttccg
    38281 cacatcagtt cgaaaccggt cagcgagtgc cgccgattgg catcaccgat cgtggcgagt
    38341 tggtgcttga taaagatcag tttagctaca aaacctggaa cagcgcgcag ttagtgggaa
    38401 aagtgcgagt actgcaacat attgctggtc gcacctctgc aaaagagaaa aacgcgacgc
    38461 tgattgaagc gattaaatca gcgaagttac cgcacgatcg ttaccagacc accaccattg
    38521 ttaacaccga cgacgcgatt ccgggttcag ggatgtttgt gcgcagcagt ctggagagta
    38581 ataaaaagct ttatccctgg tcgcagttta ttgttgatag caatggcgtc gcacgcggtg
    38641 cctggcagct ggatgaagaa agttccgctg tagtggtgct ggataaagac ggtcgcgtgc
    38701 aatgggccaa agacggggcg ctcactcaag aagaggtgca gcaagtgatg gacctgctgc
    38761 ataaattaat taataaatag acactctgaa ccccggattc aggaacgact cgcgcggagt
    38821 gtaatccagc ggtttacctt gccagtcgtg aacgtgcgct ccggcagctg cagccacagc
    38881 atgaccagcg gcggtatccc aaatattcgt tggcccgaaa cgcgggtaca gctgcgcctg
    38941 tccttccgcc accaggcaga atttcagcga agagccgatg gacgtggtct gatgttcgcc
    39001 aagctgttgc agatactctt tcagctccgc atctgcatgg gaacggctga tcaccaccag
    39061 cggcgggcgc gcatcgcgga cctgaatctg cttgcgcaca ccgcactctt ctttccaggc
    39121 tttgccttct gccgcgctgt acattacgtt cattaccggc gcatacacca cacctaaaat
    39181 cggtttgccg tggtcgatga gcgcaatgtt aacggtgaat tcgccattac gtttaataaa
    39241 ctctttagta ccatccagcg ggtctaccag ccagtaacgc tgccagtgct gacggacttc
    39301 ccaaccggga ggatcttctt cagaaaggac cggaatatcc ggtgtcagcg tacgtaaacc
    39361 gtccatgata acggtgtgag cggcaatatc cgctgccgtt accggagaat tgtccgcttt
    39421 gctgacgacg tccatcggtt ttgtcccgtc gtagacctgc ataatggcat cgcctgcatt
    39481 ccgtgcaagc tggcatactt gatctaacat ttctccacct cgtctctgtg agcggtgtta
    39541 acttattgtt ttacttatac cctatcgtta atgaatgcgc caactgtgat agtgtcatca
    39601 ttttcaaagc gtaaaattgt ggcattcttc actgttctat aagtaagacg tttattctcc
    39661 cttttctttc gtattcccga tgataaaagg atgtccctga tgattaagtt tagcgcaacg
    39721 ctcctggcca cgctgattgc cgccagtgtg aatgcagcga cggtcgatct gcgtatcatg
    39781 gaaaccactg atctgcatag caacatgatg gatttcgatt attacaaaga caccgccacg
    39841 gaaaaattcg gactggtacg tacggcaagc ctgattaacg atgcccgcaa tgaagtgaaa
    39901 aacagcgtac tggtggataa cggcgatttg attcagggga gtccgcttgc agattacatg
    39961 tcggcgaaag gtttaaaagc aggtgatatt cacccggtct ataaggcatt aaacacgctg
    40021 gactataccg tcggcacgct tggcaaccac gagtttaact acggtctgga ttacctgaaa
    40081 aatgcgctgg cgggagcgaa attcccttat gtaaatgcca acgtcattga cgccagaacc
    40141 aaacagccaa tgtttacacc gtatttaatt aaagacaccg aagtggtcga taaagacgga
    40201 aaaaaacaga cgctgaagat tggctatatt ggcgtcgtgc cgccgcaaat catgggctgg
    40261 gataaagcta atttatccgg gaaagtgacg gtgaatgata ttaccgaaac cgtgcgcaaa
    40321 tacgtgcctg aaatgcgcga gaaaggtgcc gatgtcgttg tcgttctggc acattccggg
    40381 ctgtctgccg atccgtataa agtgatggcg gaaaactcag tttattacct cagtgaaatt
    40441 ccgggcgtta acgccattat gtttggccat gctcacgccg ttttcccagg taaagatttt
    40501 gctgatatcg aaggggctga tatcgcgaaa ggcacgctga atggtgttcc ggcggtaatg
    40561 ccaggcatgt ggggcgatca tcttggtgtg gtcgacttac aactcagtaa tgacagcggt
    40621 aaatggcagg tgacgcaggc gaaagcggaa gcacggccga tttacgacat cgctaataaa
    40681 aaatccctcg cggcggaaga cagcaagctg gtagaaacac tcaaagccga tcacgatgcc
    40741 acacgccagt tcgtcagcaa gccaatcggt aaatctgccg acaatatgta tagctatctg
    40801 gcgcttgtgc aggacgatcc gaccgtgcag gtggtgaaca acgcgcaaaa agcgtatgtc
    40861 gagcattaca ttcagggcga tccggatctg gcaaaactgc cagtgctttc agctgccgca
    40921 ccgtttaaag ttggtggtcg caaaaatgac cccgcaagct atgtggaggt ggaaaaaggc
    40981 cagttgacct tccgtaatgc cgccgatctt tatctctacc ccaatacgct gattgtggtg
    41041 aaagccagcg gtaaagaggt gaaagagtgg ctggagtgct ccgccggaca gtttaaccag
    41101 attgatccta acagcacgaa accacagtca ctcatcaact gggatggttt ccgcacctat
    41161 aactttgatg ttattgatgg tgtgaattat cagattgatg ttacccagcc cgcccgttat
    41221 gacggcgagt gccagatgat taatgccaat gcggaaagga ttaagaacct gacctttaac
    41281 ggcaagccga ttgatccgaa cgccatgttc ctcgttgcca ccaataacta tcgcgcttac
    41341 ggcggcaaat ttgccggtac gggcgacagc catatcgctt ttgcttcacc ggatgagaac
    41401 cgctcggtgc tggcagcgtg gattgctgat gagtcgaaac gtgcggggga aattcacccg
    41461 gcggcagata acaactggcg tttagcaccg atagctggcg ataagaaact ggatatccgt
    41521 ttcgaaacct ctccgtcaga taaagccgca gcgtttatta aagaaaaagg gcagtatccg
    41581 atgaataaag tcgcgaccga tgatatcggg tttgcgattt atcaggtgga tctgagtaag
    41641 taaaacactt ctttttcggc ctacaaatca tcaaccgcat ccggcattta ttggcggatg
    41701 cgatgatgac gcatcttatc cgccctacaa gccatgaacc gtagtccgga taagcgcagc
    41761 gcatccggca gttatgccgc acgttcatcc cgcaccgcta acacctcggg caaattcaac
    41821 tcaatccagt ccgccagtgc agcaaccttt tcgctcacct gctcgcccag cggcgtgagg
    41881 ctatattcca catgcggcgg caccaccgga tacgcgatac ggttaagaaa accatcctgc
    41941 tctaacgcct gtaacgactg cgtaagcatc ttttcactca ccccgcccat tttacggcgc
    42001 aggtcgctaa agcgatgagt accttcgcgt agcgccacca gaatcaacac cccccaacgg
    42061 ctggtgacgt gtttcaacac ctcgcgcgac gggcactgtt ccgcaaagag atttccctct
    42121 ttcagttgtt gcgacaggct aacctgactc atttcatact tacctttttg tatgtactta
    42181 ctaaaagtaa gtttaagtgt tagcgtattt aaacacaaga caaaacgatg gagacttccc
    42241 atgatcgcta ttactggtgc cactggccaa cttggtcact atgttattga atccttgatg
    42301 aaaacggttc ctgccagcca aatagtggct atcgttcgta atccggcaaa agcacaggca
    42361 ctgacagcac aaggcattac cgtgcgtcag gctgactacg gcgatgaagc cgcactgaca
    42421 tctgcgcttc agggagtgga aaaactactg ctgatctctt ccagcgaagt gggtcaacgt
    42481 gccccgcagc atcgtaatgt tattaatgcc gcaaaggcgg ccgacgtgaa atttatcgct
    42541 tataccagcc tgctacatgc caattcctcc ccgctcggcc tcgccgatga gcacatcgag
    42601 acggagaaaa tgctggctga ttctggcatc gtttacaccc tgctgcgcaa cggctggtac
    42661 agtgaaaact acctcgccag cgccccggca gcactggaac acggcgtatt tatcggtgcc
    42721 gcgggcgatg gcaaaattgc ctcagcaacg cgggctgatt atgcggtagc tgcggcacgc
    42781 gtgattagcg aagccggtca cgaaggcaag gtttacgaac tggcgggcga tagagcctgg
    42841 acgctgacac agttagcggc agaactcacc aaacagagcg gaaaatcggt tacctatcaa
    42901 aatctgagcg aagccgattt cgctgcggcg ctgaaaagcg tcggactgcc tgatggactg
    42961 gcggatatgc tggcggattc tgacgttggc gcatcgaaag gcggtctgtt tgatgacagc
    43021 aaaacgctta gcaaattgat tggccgccca acgacaacgt tagccgaaag cgtaagccat
    43081 ctttttaatg ttaataacta gttaattaaa gtggcatcct cccgcatcct ctctgataat
    43141 gacgggatgc cgggagcaat catgtctgct tcctgaatat ttcttctgac agaccagtgg
    43201 atgccagtga tgattagcgg cgtgctgtac gccctgttag cagggttgat gtgggggctt
    43261 atttttgtcg ggccgttgat cgtgccggaa tacccggcga tgttacagtc gatggggcgt
    43321 tacctggcgc tagggttaat tgcgctgccc attgcctggc tgggacgcgt gcgtctgcgt
    43381 cagttggcgc gtcgcgactg gcttaccgcc ttgatgctca ccatgatggg caaccttatc
    43441 tattacttct gccttgccag tgccattcaa cgtactggcg cgcctgtttc cacgatgatt
    43501 atcggcaccc tgccggtggt gattcccgtt tttgccaatc tgctttatag ccagcgcgac
    43561 ggcaaactcg catggggaaa actcgccccg gcactggttt gtattggcat cggcctggcg
    43621 tgtgtgaata ttgctgagtt aaaccacgga ctccccgatt ttgactgggc acgttatacc
    43681 tcaggcatcg tgctggcgtt agtttccgtg gtctgctggg catggtatgc cctgcgcaac
    43741 gcccgctggc tgcgggaaaa tcccgacaaa catccgatga tgtgggcgac ggcgcaggcg
    43801 ctggtcacgc tgccggtttc tctcatcggc tatctcgtcg cctgttactg gctgaatatg
    43861 caaacgccgg acttctcctt accctttggc ccccgtccgc tggtgtttat tagtctgatg
    43921 gttgcgatag ccgtgctttg ctcatgggtt ggcgcactct gctggaacgt cgccagccag
    43981 cgattaccga cagtgattct cgggccgctg attgttttcg aaacgctggc aggtttgctg
    44041 tacacctttt tactccgcca gcaaatgccg ccgctaatga cgctgagcgg tatcgcgctg
    44101 ttagtgattg gcgtggtcat tgcggtcaga gcaaaaccag aaaaaccttt aactgaatct
    44161 gtctcagaaa gttgacacgc tggcagtgag ttaaataagc ctctgctacg taagggttat
    44221 agcttttgcc ttaaagatgc atttaaaatg catcttatct tattaagaat gaggtatcag
    44281 ctatggctta tcgcgaccaa cctttaggtg aactggcgct ctctattcct cgcgcttcgg
    44341 ctctgtttcg taaatatgat atggattact gctgtggcgg taagcagacg ctggcgcgcg
    44401 cggcggcacg taaagaactg gatgttgacg tcattgaagc cgaactggca aagcttgctg
    44461 aacaaccgat tgagaaaaac tggcgtagcg ccccgctggc ggaaatcatc gaccacatta
    44521 ttgtgcgcta tcacgatcgc catcgcgagc agctgccgga gctgatcctg caagcgacta
    44581 aagtcgagcg cgttcacgcc gacaaaccaa gtgtgccaaa agggctgaca aaatacctga
    44641 ctatgctgca tgaagagctt tccagccata tgatgaaaga agagcaaatc ctcttcccaa
    44701 tgatcaaaca aggcatgggc agccaggcaa tggggccaat cagcgtaatg gaaagcgagc
    44761 acgatgaagc gggcgaactg ctggaagtga tcaaacacac caccaataac gtcacaccgc
    44821 cgccggaagc ctgcaccacc tggaaagcga tgtataacgg cattaatgaa ctgattgatg
    44881 acctgatgga gcacatcagt ctggaaaaca atgtactatt tccgcgcgcg ctggcgggtg
    44941 agtgatctaa aagctggatg gcattgcgcc atccagcatg ataatgcggt tatttccgca
    45001 gttcagcagc ctgcttctta ccaataaaca gccagcccag ccccagcgcg ataaaccaca
    45061 gcggggtaac cagcagcgcc tggcgggtgt catcttccag tgtcaacaac accagaacaa
    45121 acacaaagaa cgccatacat acccagcaca tcagcttgcc gagtggcatc ttgtagatcg
    45181 acttctcatg cagatgagga cgctgtttgc gatacaccag gtacgagcaa aggataatcg
    45241 tccagacaaa cataaacaga atcgcggaaa cggttgtaat catcgtgaac gcgccaatca
    45301 cgctgggatt cacatacaac atcaccacgc cgccgagcag acagatacaa gagaaggtca
    45361 gccctttcgc gggtactgcg cgcttagaga gtttagcgaa cgctttcggt gccacacctt
    45421 cctgggccag accaaacagc atacggctgg tagagaagac accgctgtta gcggaagacg
    45481 ccgcagaggt cagcaccaca aagttgatca cgctggcagc cgcaggcaaa cctaccaaca
    45541 caaacagttc aacgaacggg cttttctccg ggactaccga actccacggc gtcacggaca
    45601 taatcacaat cagcgagaag acgtagaaca taatgatacg gatcggaatg gagttaatcg
    45661 cgcgtggcag tgatttctcc ggatctttgg tttccgcagc tgttgtacct accagctcaa
    45721 tccccacgaa agcaaaaacc gctatctgga atccggcaaa gaagccgctt aagcctttcg
    45781 ggaaccagcc gccgtcattc cacaaatgtg caaatgatgc ttccacaccg gtcggtgact
    45841 gaaagtgcat cgccaccatg accaggccga caacaatcag cgacacgatg gcgacgattt
    45901 tgatcatcgc aaaccagaac tccatctcac cgaacatttt taccgtggcg aggtttagaa
    45961 ccagtaacag aatgatcacg gataacgaag caacccagtc agaaagccca gggaaccaga
    46021 attgcgcata ggcggtaatg gcaaccacat ctgccatacc ggttacaacc cagcagaacc
    46081 agtaagtcca gccggtgaaa tatcctgccc acggcccgag taaatcggaa gcgaagtcac
    46141 taaaagattt gtattccaga ttcgaaagca gcaattcccc cattgcccgc atcacgaaaa
    46201 agagcataaa accgatgatc atataaacga aaatgatcga cggcccggca aggctaatcg
    46261 ttttgccgga ccccataaac aaccctgtac caatggcacc gccaatggca ataagctgaa
    46321 tatgtcggtt tgtgagattg cgccgtagcg actgttcagc cggagcctga tcatcggcaa
    46381 cgacttttac ctgatctacc atgttttttt cttcctgtac ctgtctgtgt tgttcaggct
    46441 ctacggcctt tagtcagtct atgatacgac gaatctgtat cgggactcat cgatattagg
    46501 taacaatacg cgggatgaat actaagattt agatctaatg ttaattttat gtttaaagtg
    46561 agtgtcatat cacggtgata acgcgaaaca gctcacaaaa atacacgctt acagggtctt
    46621 tgcaacataa aatagcgccg tttggcaaac tgaagggttt attgctgaat gcctgctccc
    46681 ctctggttcg aggggaacag aatgcgctgc ttagaggatt tccagcagtt cgacttcaaa
    46741 caccagggtg ctgaacggag ggatggacgc gcctgcgcca cgctcgccat atgccagttc
    46801 ctgcgggata gtcagttccc atttagaacc taccggcatc agagtcagtg cttcaatcca
    46861 gccagggatc acgccgttta ccgggaattc agctggctcg cctcgagcaa cggagctgtc
    46921 aaacacggtg ccgtcgatca gttttccggt gtaatgaaca cgaacgcggt cggtacgtgc
    46981 cggaattgcg ccttcgccct ggttgatcac gcggaattgc aggccagatt cggtgctatt
    47041 tacaccttct tttttggcgt tttcttccag gtatttcaca ccttcagcag ccatcgcctg
    47101 gaaacgctga cgacgaacgg catcggcgcg ttcgtggatt tcacgcagcg cgcggtgcac
    47161 cacatcaacc ggaacagccg gatgtttgcc ttccagcgca tcggcaatgc ctgcaaccag
    47221 tgcttctggc agcagccctt ccaggccaga ttcactcagc tgttgcccta cctgcaaacc
    47281 aatgccgtag cttgcttgcg cttcgatggt gtcaaaagtt ggggtggtca tcgtatttcc
    47341 tttcttaggg tataaagtag cgggcagcat atcagccaca cttctttggg taaaactttg
    47401 tcgcgggaat gatgacaaat cccggggaaa aagaaacaat agcaatatcc cgtatgaata
    47461 taataaaggc gggtttacca cggacatcat agcgttagct tattcatagg ctatgattga
    47521 ggaacaagac gcggagcagg aggaaaccat gcccgggcgc tttgaactaa aaccaaccct
    47581 ggagaaagtc tggcacgcgc cggacaattt tcgcttcatg gacccgctgc cgccgatgca
    47641 tcgtcggggg atcattattg ccgccattgt attggtagtc ggatttctgc ttccatctga
    47701 tgatacgccc aacgcgccgg tggtgacgcg tgaagcgcag ttagatattc agtcgcaatc
    47761 acaaccaccg acagaagaac agctgcgggc gcaattagtt acgccacaaa atgatcctga
    47821 tcaggtggct ccggtcgctc cagaaccaat acaggaaggc caaccagaag agcagccgca
    47881 aaccacccat acgcaaccat tccagccaga tagcggaatt gataatcaat ggcgctccta
    47941 tcgtgtcgaa cccggtaaaa cgatggcgca gctgttccgc gaccacggac tgcccgctac
    48001 cgatgtctat gccatggcgc aggtagaagg cgcggataag ccactgagta atttgcaaaa
    48061 cgggcagatg gtgaaaatcc gccagaacgc cagcggcgtg gtgacgggct taaccattga
    48121 tacggggaat aatcaacagg tcctgtttac ccgccagccg gatggcagtt ttattcgcgc
    48181 gcggtagatg aagggtcaac caaaagccag ctaaggattg tctctttgat gaccttacgt
    48241 ctggcttcca gtgcctgcgg tgaaaccata ctgatgtgat aaaccaggcc aagcgtatgc
    48301 tggttgatca aataatatcc cccaagcgca gcaatattaa tattcacctg caatgggtcg
    48361 atatctggct taaaaatatt ttgtttcttc ccttcatcca ataaggattc cattaactgc
    48421 aagtgcgcat ggttaatttc taaaagacgc tgtgattttg cataatgcac acccttactt
    48481 tgattttcac tatgcacaat ttttaaaaac cagggattag aaatataata atcccatgta
    48541 aaatcgatta ttttctcaat ggctttctgt ggcggaagat ctgagaaatc aagggccttt
    48601 tctttttgtc gaatatcgtt ccaggtatat tccagtactt cgataaataa attttcttta
    48661 ttaccaaagt ggtgataaac cgtctgttta ctgcaccccg cggccttgac gatattatcg
    48721 actctggccc cttcgtaacc atattcggca aattcattaa ccgcgcataa cagcagcttt
    48781 tctttcaggc tctgtacgtt gctggtatca agatacataa cgcttcccca cgacctcttc
    48841 gtggtgacta tcataacctg atttgatttc agcttcagaa aaaagggcaa aaacttgtac
    48901 tttcatcagg ttatcaaatg tcctgtgatc gccatcgcat atcaaccaac tagatggttg
    48961 atctttttat cctccagtcg caagctaatc atcactaagg aaacaaaatt caaaaaaaga
    49021 gagctattgt atgaaatcca ctcgtcctgt tgccgtgatt accggagccg ctcgtggtat
    49081 tggaaagggc tgtgcgctgg agcttgcgcg tggcggattt aatctgctga ttaatgatcg
    49141 tccggatgcg gatagcgtag aaaaattaca tgccacacaa caagaatgtc tggcggaagg
    49201 cgtggaagtg atttgcttcc ctgctgatgt gggcgatctc tccctgcatg aagagatgct
    49261 cgacgcagcg caaaaccagt ggggacgtct ggactgcctg ctcaacaacg caggcatttc
    49321 agtgaaaaaa cgcggtgacc ttctcgacct cgaaccagac agcttcgacc aaaatattgc
    49381 cattaacacc cgcgcaccgt tcttccttgc tcaggcattc agtaagcgct tactggcaca
    49441 gccgaaaccg gaagcagaac tgccgcaccg ctcgattatt tttgtcagtt ccatcaacgc
    49501 catcatgctg gcgatgaatc gcggcgaata taccattgcc aaaactgccg tctctgccgc
    49561 tgctcgtctg tttgctgctc gcctttgtaa tgaacaaatt ggcgtatatg aagtgcgtcc
    49621 tggtctcatc aaaaccgata tgactattcc ggcaacggcc tattacgacg aactgattgc
    49681 caaaggtctg gtgccgtggg gacgttgggg ttatccggcg gatattgctt ccactgtccg
    49741 cgcgatggcg gaaggtaaac ttatttacac ctgtggtcag gcggtcgcca tcgacggcgg
    49801 cttaagtatg ccgcgcttct gaggctgaat catgagagat ctgttacaac gcccggattt
    49861 attcagtatc aataccgcga cactgggcta taaaacgccg ctacccgcca ttattgatgc
    49921 ctgcgccgca cgcggtattg gcgcgattgc cccctggcgc agggagttgc agagtgaaga
    49981 tctccagcaa accgcccgcc agcttgctgc cagtaacatg aacgtttccg gtttgtgccg
    50041 tagcacctac tacaccgcac cgacgctggc ggagcgcaaa ctggcgattg acgataaccg
    50101 ccgggcgctg gacgacgccg ctgtactgaa cgccgcctgt tatatgcagg tggttggcgg
    50161 tctgccaatg ggaacaaaag atctgtacga agcacgggaa caggtgaaac aagggattcg
    50221 ccagttgctg ccacattcga aagaggtcgg cgtgccaatt gcactggagc cactgcaccc
    50281 gatgactgct gccaatcgtt cttgtctgtg cacgttacgt caggcgctgg actggtgtga
    50341 tgaactcgat cccgatggcg aatttggcct cggcgtggcg gtagatgtct accatgtctg
    50401 gtgggacccg gatctcgcca gtcagatcct gcgtgccgga aaacgcatcc tcgcgtttca
    50461 tgtttccgac tggttagtgc cgaccaccga tctggtcaat gaccgtggaa tgccgggaga
    50521 tggtgtcatc aatattccgt caattcgtcg gctggttgaa aacgccggat ttaatggcgc
    50581 tattgaactg gaaatattct caccgtactg gtggcagaaa gatattaaca gcacgctgga
    50641 tattagcgtc gatcgcatcg cgcattattg ttaaggaatg atcatgaatg accaacccgt
    50701 tctgttcagc agagcggcgg cttcttgccg cctgacattg aaccgtgaag ataaatgtca
    50761 tgctattaat gaagaaatga tcgaatctct cgaccattat ttaaatgaaa ttgaaaatga
    50821 cacggcatta cgcttagtcg aattaacggc aaccggcgat aaattctttt gtgcgggtgg
    50881 cgatattaaa tcgtggtcgg cctattcgcc attagatatg ggcagaaaat ggattaaacg
    50941 cggtaatgac gtttttaatc gcctgcgaaa cttaccccaa ttaaccgttg cgaatctcaa
    51001 cggtcatacc atcggcggtg gtattgaact tgccttgtgc tgcgatattc gtatcgctcg
    51061 ccccggcgcg aaattctcta acccggaagt gatgctcggc atggtgccag gctggatggg
    51121 cattgagcga gtgcttaatc aggttggtcc tgtggtcggt cgccagatgc tgatgctggg
    51181 taaacgcctg acggcgcagg aagctcagac agcgaattta attgatgaag tggtagaaaa
    51241 agaacaggtg gaaagctgga tggcgaacca gttagcgcag ctggaaaaat gtggtccggt
    51301 ggcactggca catattaagc aactgattct ggcgctggaa aataaacacg ccgattactc
    51361 gcaccagttg ctggctggat taatgtccgc cactcaggat tgccaacagg cgacgcacgc
    51421 atttgccgaa aaagccggcg tgagcttcca caatcaataa ggaaattgta tgcgtaagtt
    51481 atcaaccgcc gagacactgg cggcgcaaat tcaggacggc gcaaccattg ccattagcgg
    51541 caacggcggc ggcatggtgg aagctgacca tattctggcc gccattgaag cgcgctttct
    51601 gcaaaccgga cacccgcgcg atctgacatt aatccactcg ctgggtattg gcgatcgcga
    51661 cagcaaaggc actaaccgtt ttgctcacgc cgaaatgctc aaacgcatta ttgccggaca
    51721 ttttacctgg tcgcccaaga tgcaggaact ggtgaaaaac aatgctattg aagcctactg
    51781 tttccctggt ggcgtaattc aggcactgct gcgggagatc ggtgccggac gtccggggct
    51841 ttttacccac gttgggctgg gatcgtttgt tgatccacgc aatggtggcg gtaagtcgaa
    51901 tgaatgcact accgacgacc tggtagaact gattgaaatc gacggtgaga ccaaacttcg
    51961 ttatcgccct ttcaaagtgg attacgcgat tttgcgtggc acttatgccg atccacgagg
    52021 caacgttagc ctcgaagaag aagcgattga tatggatagc tattcgatgg cgctggcagc
    52081 acacaacagc ggcggcaaag tgttcgtaca ggtacgcgat gtgctggaag caggtaccat
    52141 tgaaccacgt cgggtcaagc taccaggaat tctggttgat ggcatcgttg agcaccgcga
    52201 acaaccgcaa acctatcttg gtggttacga cctgaccatc agcggtcaac atcgccgtct
    52261 aagttctaac gacgctattg aactggttag tcatccggtg cgtcgcctga ttgcccgtcg
    52321 ggcagcgcgg gaactggtgg caggcgcttc aaccaacttt ggctttggta ttcccggcgg
    52381 tattcctggg gttgcgctgc gcgaaggcgt gccttatcaa agtttgtggc tgagtgtaga
    52441 acagggtgtg cataacggca tgatgcttga tgatgctctg ttcggctgcg cccgtaacgc
    52501 cgatgccatt attccatcac tcgatcaatt tgaattctac agtggcggcg gcatcgatat
    52561 caccttcctc ggcatgggag agatggatca atacggtaac gtcaacgtat cccatctcaa
    52621 tggcaatctg attggccccg gcggatttct cgaaattgca caaaacgccc gtaaagtggt
    52681 gttttgcggt acgttcgacg ccaaaggcag caagattgat gtaacgccag atggcttgca
    52741 tatcgcccag tcaggtcaaa tccctaaact ggttacccag gtggaaaaaa ttacttttag
    52801 cgccgcctac gcacagcaaa gtggtcagga agtgttgtat atcactgaac gcgcagtatt
    52861 ccagttaacg gcagaaggcg ttgaattaat tgaaatcgcg ccgggtgtgg agattgagcg
    52921 cgacattctg ccgtatatgg ccttccgtcc gattatcagg cacccacgtc tgatggaaag
    52981 tagcctgttt atgccaatgg aggatgcatg agtcagcttg atgacaccat ccttgatgca
    53041 ctgacgcacg ttactttccc aaagggtttt gcacaggcag aacccgcatg ggttgtcacg
    53101 gttgacggcg ttgattaccc actctggaaa acagatgccc tggtagtcgg cagtggtgca
    53161 gccgggctac gtgcagctgt ggaacttaaa cgccgccagc aaaatgtgtt aatcgccacc
    53221 gccgggttat atatggggac gtcggcatgt tctggctctg ataaacaaac actgtttacc
    53281 gccgctactg cgggcaacgg cgacaacttc accaaactgg cagaggcact ggcgagcggt
    53341 ggggcgatgg atcacgacac cgcttatgtt gaagcggtag gttctctgca cactcttggc
    53401 gggttgcaat atcttggtct ggaattaccg gaagatcgct atggcgcgat tcttcgttat
    53461 caaaccgacc atgacgaagc cgggcgtgca acctcgtgtg ggccgcgtac ctcaaggtta
    53521 atggtgaaag tgctgctgga agaagtacag cgtcttgcta tcccaatgtt gaccagtgca
    53581 acagtgatta aactgctgca tcagcgtgac gaaaacggtg aagaccgtgt ggcgggggca
    53641 atcctcgcga ccggtcatcg cgcccataac ccatgggggc tggcaattgt gactgcgccc
    53701 aatgtggtac tggcaacagg agggcctggc gagctttatc gcgacagtgt ttatccacac
    53761 aaatgtttcg gctcgctggg tctggcgctg gaggaaggtc tgacgctaac caatctgact
    53821 gaaagccagt ttggtatcgg cacaccgcgc agcacgtttc cgtggaattt gtccggcacc
    53881 tatgtgcagg tgatcccgta tatctattcc gtagatgctg agggtaacga gtataacttc
    53941 ctcgcggatt actatcgcac cacccaggag ctagcttcaa acatcttccg taaaggctat
    54001 cagtggccgt tccacgccac gcgggtaatg gattttggct ccagcttgtt agatatggca
    54061 gtagcgcaag agcagcaatc agggcgtcag gtatttatgg atttcaatcg caatcctgaa
    54121 gcggtgccgg gtgacctgcc cttctcatta gatcgactgg acgacgacgt tcgcacgtat
    54181 ctggaaaata acgacgctct ggcaccatcg cccatcgaac gcctgcaacg aatgaatccg
    54241 ctgtctatct cgctgtataa gatgcacggt tacgatctca ccacgcagcc attgcagttt
    54301 gccatgaata atcagcacat gaatggcggc attgaagtcg atatctgggg acaaacatct
    54361 ctgcccggtt gttttgcggt gggggaagtt gctggcacac acggcgtcac ccgacctggc
    54421 ggcgcggcat tgaatgccgg gcaggttttt gctgttcgtc tggcacgttt tattggttgc
    54481 acgcaaaagc ggaatattga tggagatata gcacagctgg cagctccggc actggcttct
    54541 ataagagaga tcattactca ggcgcacgat aacgggaccg ggatgccgtt gtcggttgtg
    54601 agagaaaaaa ttcaggcacg aatgtctgac catgcgggat ttatttgcca tgccgataaa
    54661 gtccgacgcg ccactcgtga tgccctgcta ttgaacgaat ttgtccaacg acatggattg
    54721 gcaatcaaac atgtgggtga agttgccgag ctgtttatgt ggcggcatat gacgctgact
    54781 tctgccgcgg tattaacaca attaacgcat tatattgatg ctggtggtgg tagccgtggg
    54841 gcgcgtatgg ttattgatcc acaaggcaaa tgcctaccac aaactcgtcg cggcgcaaaa
    54901 gaagaatggc gttttcgctc tgaactcgct gaagacaaaa atcacaaatt aacaattcaa
    54961 tattcgcaag gttcttttat taccgaagta aagtctttac gtatgcaacc gtgtattaac
    55021 ggtatttact ttgaaaaaaa ctggccagac tttttaaagg gagacattta cacacaataa
    55081 tttccaatat ttcctctgta cctgattttt attgcctatt
//
LOCUS       DAHHTG010000014        51852 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.014, whole genome shotgun sequence.
ACCESSION   DAHHTG010000014 DAHHTG010000000
VERSION     DAHHTG010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 51852)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 51852)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..51852
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.014"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            <1..370
                     /locus_tag="NBI74_000846"
     CDS             <1..370
                     /locus_tag="NBI74_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001525915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1931219.1"
                     /translation="YPFYKHTKPEYKEYDWEQIQGIAFFAQNHHSTYATIRCIVYESL
                     DSTNIIDTFNLITTESATNALHMTNNKLWLWINSFMNFDDEFLEKSINTAPGIWGREV
                     NWPDDMNKKSMLSSMDEYIL"
     gene            complement(436..726)
                     /gene="ilvN"
                     /locus_tag="NBI74_000847"
     CDS             complement(436..726)
                     /gene="ilvN"
                     /locus_tag="NBI74_000847"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HCD1931220.1"
                     /translation="MQNTTHDNVILELTVRNHPGVMTHVCGLFARRAFNVEGILCLPI
                     QDSDKSHIWLLVNDDQRLEQMISQIDKLEDVVKVQRNQSDPTMFNKIAVFFQ"
     gene            complement(730..2418)
                     /gene="ilvB"
                     /locus_tag="NBI74_000848"
     CDS             complement(730..2418)
                     /gene="ilvB"
                     /locus_tag="NBI74_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HCD1931221.1"
                     /translation="MASSGTTSTRKRFTGAEFIVHFLEQQGIKIVTGIPGGSILPVYD
                     ALSQSTQIRHILARHEQGAGFIAQGMARTDGKPAVCMACSGPGATNLVTAIADARLDS
                     IPLICITGQVSASMIGTDAFQEVDTYGISIPITKHNYLVRHIEELPQVMSDAFSIAQS
                     GRPGPVWIDIPKDVQTAVFEIETQPAMAEKAAAPAFSEESIRDAAAMINAAKHPVLYL
                     GGGVINAPARVRELAEKAQLPTTMTLMALGMLPKAHPLSLGMLGMHGVRSTNYILQEA
                     DLLIVLGARFDDRAIGKTEQFCPNAKIIHVDIDRAELGKIKQPHVAIQADVDDVLAQL
                     IPLVEAQPRAEWHQLVADLQREFPCPIPKACDPLSHYGLINAVAACVDDNAIITTDVG
                     QHQMWTAQAYPLNRPRQWLTSGGLGTMGFGLPAAIGAALANPDRKVLCFSGDGSLMMN
                     IQEMATASENQLDVKIILMNNEALGLVHQQQSLFYKQGVFAATYPGKINFMQIAAGFG
                     LETCDLNNEADPQAALQEIINRPGPALIHVRIDAEEKVYPMVPPGAANTEMVGE"
     gene            complement(2524..2622)
                     /gene="ivbL"
                     /locus_tag="NBI74_000849"
     CDS             complement(2524..2622)
                     /gene="ivbL"
                     /locus_tag="NBI74_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ilvB operon leader peptide IvbL"
                     /protein_id="HCD1931222.1"
                     /translation="MTTSMFNAKLLPTAPSAAVVVVRVVVVVGNAP"
     gene            3023..4207
                     /gene="emrD"
                     /locus_tag="NBI74_000850"
     CDS             3023..4207
                     /gene="emrD"
                     /locus_tag="NBI74_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EmrD"
                     /protein_id="HCD1931223.1"
                     /translation="MKRHRNVNLLLMLVLLVAVGQMAQTIYIPAIADMARDLNVREGA
                     VQSVMGAYLLTYGVSQLFYGPISDRVGRRPVILVGMSIFMLATLVAVSTSSLMVLIAA
                     SAMQGMGTGVGGVMARTLPRDLYERTQLRHANSLLNMGILVSPLLAPLIGGLLDTMWN
                     WRACYLFLLVLCAGVTFSMARWMPETRPVDAPRTRLLTSYKTLFGNSGFNCYLLMLIG
                     GLAGIAAFEACSGVLMGAVLGLSSMTVSILFILPIPAAFFGAWFAGRPNKRFSTLMWQ
                     SVICCLLAGLLMWIPDWFGVMNVWTLLIPAALFFFGAGMLFPLATSGAMEPFPFLAGT
                     AGALVGGLQNIGSGVLASLSAMLPQTGQGSLGLLMTLMGLLIVLCWLPLATRMSHQGQ
                     PV"
     gene            complement(4215..4712)
                     /locus_tag="NBI74_000851"
     CDS             complement(4215..4712)
                     /locus_tag="NBI74_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HCD1931224.1"
                     /translation="MTGSQVIDAEEDRHKLVVEYKDALQPADFYHNFKQLGIRSVQLI
                     PHLEFDDRGDLTAASVTAELWGKFLIALFECWVRADISRISIELFDTTLQKWCGSEKS
                     QPRRDCQACDWHRLCPHAREDKPDNVLCAGYQAFYSYSAPHMRVMRDLIKQHRSPMEL
                     MTMLR"
     gene            complement(4709..5071)
                     /locus_tag="NBI74_000852"
     CDS             complement(4709..5071)
                     /locus_tag="NBI74_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF202 domain-containing protein"
                     /protein_id="HCD1931225.1"
                     /translation="MPDSRKARRIADPGLQPERTSLAWFRTMLGYGALMALAIKHNWH
                     QAGMLFWISIGILAIVALILWHYTRNRNLMDVTNSDFSQFHVVRDKFLISLAVLSLAI
                     LFAVTHIHQLIVFIERVA"
     gene            complement(5061..5408)
                     /locus_tag="NBI74_000853"
     CDS             complement(5061..5408)
                     /locus_tag="NBI74_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidH family protein"
                     /protein_id="HCD1931226.1"
                     /translation="MKISRLGEAPDYRFSLANERTFLAWIRTALGFLAAGVGLDQLAP
                     DFATPVIRELLALLLCLFSGGLAMYGYLRWLRNEKAMRLKEDLPYTNSLFIISLILMV
                     VAVIVMGLVLYAG"
     gene            complement(5468..6961)
                     /locus_tag="NBI74_000854"
     CDS             complement(5468..6961)
                     /locus_tag="NBI74_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HCD1931227.1"
                     /translation="MKRPNFLFIMTDTQATNMVGCYSGKPLNTQNIDSLAAEGIRFNS
                     AYTCSPVCTPARAGLFTGIYANQSGPWTNNVAPGKNISTMGRYFKDAGYHTCYIGKWH
                     LDGHDYFGTGECPPEWDADYWFDGANYLSELTEKEISLWRNGLNSVEDLQANHIDETF
                     TWAHRISNRAVDFLQQPARADEPFLMVISYDEPHHPFTCPVEYLEKYTDFYYELGEKA
                     EDDLANKPEHHRLWAQAMPSPVGDDGLYHHPLYFACNDFVDDQIGRVINALTPEQREN
                     TWVIYTSDHGEMMGAHKLISKGAAMYDDITRIPLIIRSPQGERRQVDTPVSHIDLLPT
                     MMALADIEKPEILPGENILAVKEPRGVLVEFNRYEIEHDSFGGFIPVRCWVTDDFKLV
                     LNLFTSDELYDRRNDPNEMHNLIDDIHFADVRSKMHDALLDYMDKIRDPFRSYQWNLR
                     PWRKDAQPRWMGAFRPRPQDGYSPVVRDYDTGLPTQGVKVEEKKQKF"
     gene            complement(6958..8673)
                     /locus_tag="NBI74_000855"
     CDS             complement(6958..8673)
                     /locus_tag="NBI74_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="solute:sodium symporter family transporter"
                     /protein_id="HCD1931228.1"
                     /translation="MNSLQILSFVGFTLLVAVITWWKVRKTDTGSQQGYFLAGRSLKA
                     PVIAASLMLTNLSTEQLVGLSGQAYKSGMSVMGWEVTSAVTLIFLALIFLPRYLKRGI
                     ATIPDFLEERYDKTTRIIIDFCFLIATGVCFLPIVLYSGALALNSLFHVGESLQLSDG
                     AAIWLLVILLGLAGILYAVIGGLRAMAVADSINGIGLVIGGLMVPVFGLIAMGKGSFM
                     QGIEQLTTVHAEKLNSVGGPTDPLPIGAAFTGLILVNTFYWCTNQGIVQRTLASKSLA
                     EGQKGALLTAVLKMLDPLVLVLPGLIAFHLYQDLPKADMAYPTLVNNVLPVPLVGFFG
                     AVLFGAVISTFNGFLNSASTLFSMGIYRRIINQNAEPQQLVTVGRKFGFFIAIVSVMV
                     APWIANAPQGLYSWMKQLNGIYNVPLVTIIIMGFFFPRIPALAAKVAMGIGIISYITI
                     NYLVKFDFHFLYVLACTFCINVVVMLVIGFIKPRATPFTFKDAFAVDMKPWKNVKTAS
                     IGILFAMIGVYAGLAEFGGYGTRWLAMISYFIAAVVIVYLIFDSWRHRHDPAVTFTPD
                     AKDSL"
     gene            8840..9706
                     /locus_tag="NBI74_000856"
     CDS             8840..9706
                     /locus_tag="NBI74_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418136.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HCD1931229.1"
                     /translation="MNGKLQSSDVKNETPYNIPLLINENVISSGISLISLWHTYADEH
                     YRVIWPRDKKKPLIANSWVAVYTVQGCGKILLKNDEQITLHGNCIIFLKPMDIHSYHC
                     EGLVWEQYWMEFTPTSMMDIPVGQQSVIYNGEVYNQELTEVAELITSPEAIKNNLAVA
                     FLTKIIYQWICLMHAVGKKDPQRRQIEKLIATLHASLQQRWSVADMAATIPCSEAWLR
                     RLFLRYTGKTPKEYYLDARLDLALSLLKQQGNSVGEVADTLNFFDSFHFSKAFKHKFG
                     YAPSAVLKNTDR"
     gene            complement(9796..11457)
                     /locus_tag="NBI74_000857"
     CDS             complement(9796..11457)
                     /locus_tag="NBI74_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative transporter"
                     /protein_id="HCD1931230.1"
                     /translation="MSDIALTVSILALVAVVGLFIGNVKFRGVGLGIGGVLFGGIIVG
                     HFVSQAGMTLSSDMLHVIQEFGLILFVYTIGIQVGPGFFASLRVSGLRLNLFAVLIVI
                     IGGLVTAILHKLFDIPLPVVLGIFSGAVTNTPALGAGQQILRDLGTPMAMVDQMGMSY
                     AMAYPFGICGILFTMWMLRVIFRVNVETEAQQHESTRTNGGALIRTINIRVENPNLHN
                     LAIKDVPILNGDKVICSRLKREETLKVPSPETVIQLGDLLHLVGQPADLHNAQLVIGQ
                     EVDTSLSTKGTDLRVERVVVTNENVLGKRIRDLHFKERYDVVISRLNRAGVELVASSD
                     ISLQFGDILNLVGRPSAIDAVANVLGNAQQKLQQVQMLPVFIGIGLGVLLGSIPVFVP
                     GFPAALKLGLAGGPLIMALILGRIGSIGKLYWFMPPSANLALRELGIVLFLSVVGLKS
                     GGDFIHTLVDGEGLSWIGYGALITAVPLITVGILARMLAKMNYLTMCGMLAGSMTDPP
                     ALAFANNLHPTSGAAALSYATVYPLVMFLRIITPQLLAVLFWSIG"
     gene            complement(11655..12083)
                     /gene="ibpB"
                     /locus_tag="NBI74_000858"
     CDS             complement(11655..12083)
                     /gene="ibpB"
                     /locus_tag="NBI74_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock chaperone IbpB"
                     /protein_id="HCD1931231.1"
                     /translation="MRNFDLSPLMRQWIGFDKLANALQNAGESQSFPPYNIEKSDDNH
                     YRITLALAGFRQEDLEIQLEGTRLSVKGTPEQPKEEKKWLHQGLMNQPFSLSFTLAEN
                     MEVSGATFVNGLLHIDLIRNEPEPIAAQRIAISERPALNS"
     gene            complement(12195..12608)
                     /gene="ibpA"
                     /locus_tag="NBI74_000859"
     CDS             complement(12195..12608)
                     /gene="ibpA"
                     /locus_tag="NBI74_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006687722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock chaperone IbpA"
                     /protein_id="HCD1931232.1"
                     /translation="MRNFDLSPLYRSAIGFDRLFNHLENNQSQSNGGYPPYNVELVDE
                     NHYRIAIAVAGFAESELEITAQDNLLVVKGAHADEQKERTYLYQGIAERNFERKFQLA
                     ENIHVRGANLVNGLLYIDLERVIPEAKKPRRIEIN"
     misc_feature    complement(12602..12674)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01832"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ROSE (Repression Of Heat Shock gene Expression)
                     occurs in the 5'-region of heat shock genes and acts as an
                     RNA thermometer to modulate expression; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF01832"
     gene            12914..13246
                     /locus_tag="NBI74_000860"
     CDS             12914..13246
                     /locus_tag="NBI74_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HCD1931233.1"
                     /translation="MIKNVLLAFMICSGMTLLGGCSSVMSHTGGKEGTYPGTRASATM
                     IGDDETNWGTKSLAILDMPFTAVLDTILLPWDVFRKDSSVRSRVEKSEADAQETNNVI
                     PPAKMPAN"
     gene            complement(13250..14464)
                     /locus_tag="NBI74_000861"
     CDS             complement(13250..14464)
                     /locus_tag="NBI74_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312656.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3748 domain-containing protein"
                     /protein_id="HCD1931234.1"
                     /translation="MKQITFAPRNHLLTNTNTWTPDSQWLVFDVRPSGASFTGETIER
                     VNIHTGEVEVIYRASQGAYVGVVTVHPKSEKYVFIHGPENPDETWYYDFHHRRGVIVE
                     SGKVSNLDAMDITAPYTPGALRGGSHVHVFSPNGERVSFTYNDHVMHELDPALDLRNV
                     GVAAPFGPVNVQKQHPREYSGSHWCVLVSKTTPTPQPGSDEINRAYEEGWVGNHALAF
                     IGDTLSPKGEKVPELFIVELPQDEAGWKVAGDAPLSGTETTLPAPPRGVVQRRLTFTH
                     HRASPGLVNVPRHWVRCNPQGTQIAFLMRDDNGIVQLWLISPQGGEPRQLTHNKTDIQ
                     SAFNWHPSGEWLGFVLDNRIACAHAQSGEVEYLTENHANPPSADAVVFSPDGQWLAWM
                     EDGQLWITETDR"
     gene            14565..15665
                     /gene="cbrA"
                     /locus_tag="NBI74_000862"
     CDS             14565..15665
                     /gene="cbrA"
                     /locus_tag="NBI74_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin M resistance protein"
                     /protein_id="HCD1931235.1"
                     /translation="MEHFDVAIIGLGPAGSALARKLAGKMQVIALDKKHQCGTEGFSK
                     PCGGLLAPDAQRSFIRDGLTLPVDVIANPQIFSVKTVDVAASLTRNYQRSYININRHA
                     FDLWMKSLIPASVEVYHDSLCRKIWREDDKWHVIFRADGWEQHITARYLVGADGANSM
                     VRRHLYPDHQIRKYVAIQQWFAEKHPVPFYSCIFDNAITDCYSWSISKDGYFIFGGAY
                     PMKDGQTRFTTLKEKMSAFQFQFGKAVKSEKCTVLFPSRWQDFVCGKDNAFLIGEAAG
                     FISASSLEGISYALDSAEILRSVLLKQPEKLNTAYWRATRKLRLKLFGKIVKSRCLTA
                     PALRKWIMRSGVAHIPQLKDYPTRFTSPTSRM"
     gene            complement(15626..16963)
                     /locus_tag="NBI74_000863"
     CDS             complement(15626..16963)
                     /locus_tag="NBI74_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCD1931236.1"
                     /translation="MVSGFAMPKIRRKLAMDIPVNAAKPGRRRYLTLVMIFITVVICY
                     VDRANLAVASAHIQEEFGITKAQMGYVFSAFAWLYTLCQIPGGWFLDRVGSRVTYFIA
                     IFGWSVATLFQGFATGLMSLIGLRAITGIFEAPAFPTNNRMVTSWFPEHERASAVGFY
                     TSGQFVGLAFLTPLLIWIQEMLSWHWVFIVTGGIGIIWSLIWFKVYQPPRLTKGISKA
                     ELDYIRDGGGLVDGDAPVKKEARQPLTAKDWKLVFHRKLIGVYLGQFAVASTLWFFLT
                     WFPNYLTQEKGITALKAGFMTTVPFLAAFIGVLLSGWVADLLVRKGFSLGFARKTPII
                     CGLLISTCIMGANYTNDPMMIMCLMALAFFGNGFASITWSLVSSLAPMRLIGLTGGVF
                     NFAGGLGGITVPLVVGYLAQGYGFAPALVYISAVALIGALSYILLVGDVKRVG"
     gene            complement(17038..18186)
                     /gene="dgoD"
                     /locus_tag="NBI74_000864"
     CDS             complement(17038..18186)
                     /gene="dgoD"
                     /locus_tag="NBI74_000864"
                     /EC_number="4.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020077623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactonate dehydratase"
                     /protein_id="HCD1931237.1"
                     /translation="MKITKITTYRLPPRWMFLKIETDEGVVGWGEPVIEGRARTVEAA
                     VHELSDYLIGQDPSRINDLWQVMYRAGFYRGGPILMSAIAGIDQALWDIKGKVLNAPV
                     WQLMGGLVRDKIKAYSWVGGDRPADVIDGIKTLREIGFDTFKLNGCEELGLIDNSRAV
                     DAAVNTVAQIREAFGNQIEFGLDFHGRVSAPMAKVLIKELEPYRPLFIEEPVLAEQAE
                     YYPKLAAQTHIPLAAGERMFSRFDFKRVLEAGGISILQPDLSHAGGITECYKIAGMAE
                     AYDVTLAPHCPLGPIALAACLHIDFVSYNAVLQEQSMGIHYNKGAELLDFVKNKEDFS
                     MVGGFFKPLTKPGLGVEIDEAKVIEFSKNAPDWRNPLWRHEDNSVAEW"
     gene            complement(18183..18800)
                     /gene="dgoA"
                     /locus_tag="NBI74_000865"
     CDS             complement(18183..18800)
                     /gene="dgoA"
                     /locus_tag="NBI74_000865"
                     /EC_number="4.1.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-6-phosphogalactonate aldolase"
                     /protein_id="HCD1931238.1"
                     /translation="MQWQTKLPLIAILRGITPDEALAHVGAVIDAGFDAVEIPLNSPQ
                     WEQSIPAIVDAYGDKALIGAGTVLKPEQVDALARMGCQLIVTPNIHSEVIRRAVGYGM
                     TVCPGCATATEAFTALDAGAQALKIFPSSAFGPQYIKALKAVLPPNIAVFAVGGVTPE
                     NLAQWIDAGCAGAGLGSDLYRAGQSVERTAQQAAAFVKAYREAVQ"
     gene            complement(18784..19662)
                     /gene="dgoK"
                     /locus_tag="NBI74_000866"
     CDS             complement(18784..19662)
                     /gene="dgoK"
                     /locus_tag="NBI74_000866"
                     /EC_number="2.7.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygalactonokinase"
                     /protein_id="HCD1931239.1"
                     /translation="MTARYIAIDWGSTNLRAWLYQGDHCLDSRQSEAGVTRLNGKSPA
                     AVLAEVTTDWREENTPVVMAGMVGSNVGWKVAPYLSIPARFSSIGEQLTSVGDNIWII
                     PGLCVSHDDNHNVMRGEETQLIGARTLAPSSLYVMPGTHCKWVQADSQQINDFRTVMT
                     GELHHLLLNHSLIGAGLPPQENAVDAFAAGLERGLNAPAVLPQLFEVRASHVLGTLPR
                     EQVSEFLSGLLIGAEVASMRDYVAHQQVITLVAGTSLTARYQQAFQAMGCDVTAVAGD
                     TAFQAGIRNIAHAVAN"
     gene            complement(19659..20348)
                     /gene="dgoR"
                     /locus_tag="NBI74_000867"
     CDS             complement(19659..20348)
                     /gene="dgoR"
                     /locus_tag="NBI74_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-galactonate utilization transcriptional
                     regulator DgoR"
                     /protein_id="HCD1931240.1"
                     /translation="MTLNKTDRIVITLGKQIVHGKYVPGSPLPAEAELCEEFATSRNI
                     IREVFRSLMAKRLIEMKRYRGAFVAPRNQWNYLDTDVLQWVLENDYDPRLISAMSEVR
                     NLVEPAIARWAAERATSSDLAQIESALNEMIANNQDREAFNEADIRYHEAVLQSVHNP
                     VLQQLSIAISSLQRAVFERTWMGDEANMPQTLQEHKALFDAIRHQDGDAAEQAALTMI
                     ASSTRRLKEIT"
     gene            20627..21283
                     /locus_tag="NBI74_000868"
     CDS             20627..21283
                     /locus_tag="NBI74_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016159927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1931241.1"
                     /translation="MKLNFKGFFKAAGLCSLAFALTGCITWGLVSNTASRPHAEQWRS
                     DTIKGLSLAEDSNGTKGYVFVGESLDYLLTTGGDEVVNMLNDPAIHGERITVSDNAKF
                     ILSSSNKNFSGAITLYYDWNNEEDKALATQYGFICDTRRCTWMLDGLKGSIHQKNKKA
                     DYSNVMVFHQPFTVGFYEYKATDGVPRGLVNALLPVTLTLDIVTSPLQFLILCTTRNC
                     "
     gene            complement(21327..22139)
                     /gene="yidA"
                     /locus_tag="NBI74_000869"
     CDS             complement(21327..22139)
                     /gene="yidA"
                     /locus_tag="NBI74_000869"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HCD1931242.1"
                     /translation="MAIKLIAIDMDGTLLLPDHTISPAVKNAIAAARARGVNVVLTTG
                     RPYAGVHNYLKELHMEQPGDYCITYNGALVQKAADGSTVAQTALSYDDYRFLEKLSRE
                     VGSHFHALDRTTLYTANRDISYYTVHESFVATIPLVFCEAEKMDPNTQFLKVMMIDEP
                     AILDQAIARIPQEVKEKYTVLKSAPYFLEILDKRVNKGTGVKSLADVLGIKPEEIMAI
                     GDQENDIAMIEYAGVGVAMDNAIPSVKEVANFVTKSNLEDGVAFAIEKYVLN"
     gene            complement(22253..22651)
                     /locus_tag="NBI74_000870"
     CDS             complement(22253..22651)
                     /locus_tag="NBI74_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418153.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidB family protein"
                     /protein_id="HCD1931243.1"
                     /translation="MGLFDEVVGAFLKGDAGKYQAILSWVEEQGGIQVLLEKLQSGGL
                     GAILSTWLSNQQSNQSVSGEQVESALGTNAVSDLGQKLGVDTSTASSLLAEQLPKIID
                     ALSPQGEVSPQANNDLLSAGMELLKGKLFR"
     gene            complement(22795..25209)
                     /gene="gyrB"
                     /locus_tag="NBI74_000871"
     CDS             complement(22795..25209)
                     /gene="gyrB"
                     /locus_tag="NBI74_000871"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HCD1931244.1"
                     /translation="MSNSYDSSSIKVLKGLDAVRKRPGMYIGDTDDGTGLHHMVFEVV
                     DNAIDEALAGHCKEIIVTIHADNSVSVQDDGRGIPTGIHPEEGVSAAEVIMTVLHAGG
                     KFDDNSYKVSGGLHGVGVSVVNALSQKLELVIQREGKIHRQIYEHGVPQAPLAVTGET
                     EKTGTMVRFWPSLETFTNVTEFEYEILAKRLRELSFLNSGVSIRLRDKRDGKEDHFHY
                     EGGIKAFVEYLNKNKTPIHPNIFYFSTEKDGIGVEVALQWNDGFQENIYCFTNNIPQR
                     DGGTHLAGFRAAMTRTLNAYMDKEGYSKKAKVSATGDDAREGLIAVVSVKVPDPKFSS
                     QTKDKLVSSEVKSAVEQQMNELLAEYLLENPTDAKIVVGKIIDAARAREAARRAREMT
                     RRKGALDLAGLPGKLADCQERDPALSELYLVEGDSAGGSAKQGRNRKNQAILPLKGKI
                     LNVEKARFDKMLSSQEVATLITALGCGIGRDEYNPDKLRYHSIIIMTDADVDGSHIRT
                     LLLTFFYRQMPEIVERGHVYIAQPPLYKVKKGKQEQYIKDDEAMDQYQISIALDGATL
                     HTNASAPALAGEALEKLVSEYNATQKMINRMERRYPKAMLKELIYQPTLTETDLSDEQ
                     TVTRWVNALVSELNDKEQHGSQWKFDVHTNAEQNLFEPIVRVRTHGVDTDYPLDHEFI
                     TGGEYRRICTLGEKLRGLLEEDAFIERGERRQPVASFEQALDWLVKESRRGLSIQRYK
                     GLGEMNPEQLWETTMDPESRRMLRVTVKDAIAADQLFTTLMGDAVEPRRAFIEENALK
                     AANIDI"
     gene            complement(25238..26311)
                     /gene="recF"
                     /locus_tag="NBI74_000872"
     CDS             complement(25238..26311)
                     /gene="recF"
                     /locus_tag="NBI74_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HCD1931245.1"
                     /translation="MSLTRLLIRDFRNIETADLALSPGFNFLVGANGSGKTSVLEAIY
                     TLGHGRAFRSLQIGRVIRHEQEAFVLHGRLQGEERETAIGLTKDKQGDSKVRIDGTDG
                     HKVAELAHLMPMQLITPEGFTLLNGGPKYRRAFLDWGCFHNEPGFFTAWSNLKRLLKQ
                     RNAALRQVTRYEQLRPWDKELIPLAEQISTWRAEYSAGIAADMADTCKQFLPEFSLTF
                     SFQRGWEKETEYAEVLERNFERDRQLTYTAHGPHKADLRIRADGAPVEDTLSRGQLKL
                     LMCALRLAQGEFLTRESGRRCLYLIDDFASELDDERRGLLASRLKATQSQVFVSAISA
                     EHVIDMSDENSKMFTVEKGKITD"
     gene            complement(26311..27411)
                     /gene="dnaN"
                     /locus_tag="NBI74_000873"
     CDS             complement(26311..27411)
                     /gene="dnaN"
                     /locus_tag="NBI74_000873"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006177590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HCD1931246.1"
                     /translation="MKFTVEREHLLKPLQQVSGPLGGRPTLPILGNLLLQVADGTLSL
                     TGTDLEMEMVARVALVQPHEPGATTVPARKFFDICRGLPEGAEIAVQLEGERMLVRSG
                     RSRFSLSTLPAADFPNLDDWQSEVEFTLPQATMKRLIEATQFSMAHQDVRYYLNGMLF
                     ETEGEELRTVATDGHRLAVCSMPIGQSLPSHSVIVPRKGVIELMRMLDGGDNPLRVQI
                     GSNNIRAHVGDFIFTSKLVDGRFPDYRRVLPKNPDKHLEAGCDLLKQAFARAAILSNE
                     KFRGVRLYVSENQLKITANNPEQEEAEEILDVTYSGAEMEIGFNVSYVLDVLNALKCE
                     NVRMMLTDSVSSVQIEDAASQSAAYVVMPMRL"
     gene            complement(27416..28819)
                     /gene="dnaA"
                     /locus_tag="NBI74_000874"
     CDS             complement(27416..28819)
                     /gene="dnaA"
                     /locus_tag="NBI74_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HCD1931247.1"
                     /translation="MSLSLWQQCLARLQDELPATEFSMWIRPLQAELSDNTLALYAPN
                     RFVLDWVRDKYLNNINGLLTSFCGADAPQLRFEVGTKPVTQTPQAAVTSNVAAPAQVA
                     QTQPQRAAPSTRSGWDNVPAPAEPTYRSNVNVKHTFDNFVEGKSNQLARAAARQVADN
                     PGGAYNPLFLYGGTGLGKTHLLHAVGNGIMARKPNAKVVYMHSERFVQDMVKALQNNA
                     IEEFKRYYRSVDALLIDDIQFFANKERSQEEFFHTFNALLEGNQQIILTSDRYPKEIN
                     GVEDRLKSRFGWGLTVAIEPPELETRVAILMKKADENDIRLPGEVAFFIAKRLRSNVR
                     ELEGALNRVIANANFTGRAITIDFVREALRDLLALQEKLVTIDNIQKTVAEYYKIKVA
                     DLLSKRRSRSVARPRQMAMALAKELTNHSLPEIGDAFGGRDHTTVLHACRKIEQLREE
                     SHDIKEDFSNLIRTLSS"
     gene            29116..29196
                     /locus_tag="NBI74_000875"
     CDS             29116..29196
                     /locus_tag="NBI74_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1931248.1"
                     /translation="MTIDKKWLNRSNKDPGRSLRFTRQPV"
     gene            29426..29566
                     /gene="rpmH"
                     /locus_tag="NBI74_000876"
     CDS             29426..29566
                     /gene="rpmH"
                     /locus_tag="NBI74_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001686914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HCD1931249.1"
                     /translation="MKRTFQPSVLKRNRSHGFRARMATKNGRQVLARRRAKGRARLTV
                     SK"
     gene            29583..29942
                     /gene="rnpA"
                     /locus_tag="NBI74_000877"
     CDS             29583..29942
                     /gene="rnpA"
                     /locus_tag="NBI74_000877"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014172301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HCD1931250.1"
                     /translation="MVKLAFPRELRLLTPSQFTFVFQQPQRAGTPQITILGRLNSLGH
                     PRIGLTVAKKNVRRAHERNRIKRLTRESFRLRQHELPAMDFVVVAKKGVADLDNRALS
                     EALEKLWRRHCRLARGS"
     gene            29906..30163
                     /gene="yidD"
                     /locus_tag="NBI74_000878"
     CDS             29906..30163
                     /gene="yidD"
                     /locus_tag="NBI74_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HCD1931251.1"
                     /translation="MAPPLSPGSRVLIALIRVYQRLISPLLGPHCRFTPTCSSYGIEA
                     LRRFGVIKGSWLTVKRVLKCHPLHPGGDDPVPPGPFDTREH"
     gene            30166..31812
                     /gene="yidC"
                     /locus_tag="NBI74_000879"
     CDS             30166..31812
                     /gene="yidC"
                     /locus_tag="NBI74_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HCD1931252.1"
                     /translation="MDSQRNLLVIALLFVSFMIWQAWEQDKNPQPQAQQTTQTTTTAA
                     GSAADQGVPASGQGKLISVKTDVLDLTINTRGGDVEQALLPAYPKELNSTQPFQLLET
                     SPQFIYQAQSGLTGRDGPDNPANGPRPLYNVEKDAYVLAEGQNELQVPMTYTDAAGNT
                     FTKTFVLKRGDYAVNVNYNVQNAGEKPLEISTFGQLKQSITLPPHLDTGSSNFALHTF
                     RGAAYSTPDEKYEKYKFDTIADNENLNISSKGGWVAMLQQYFATAWIPHNDGTNNFYT
                     ANLGNGIAAIGYKSQPVLVQPGQTGAMNSTLWVGPEIQDKMAAVAPHLDLTVDYGWLW
                     FISQPLFKLLKWIHSFVGNWGFSIIIITFIVRGIMYPLTKAQYTSMAKMRMLQPKIQA
                     MRERLGDDKQRISQEMMALYKAEKVNPLGGCFPLLIQMPIFLALYYMLMGSVELRQAP
                     FALWIHDLSAQDPYYILPILMGVTMFFIQKMSPTTVTDPMQQKIMTFMPVIFTVFFLW
                     FPSGLVLYYIVSNLVTIIQQQLIYRGLEKRGLHSREKKKS"
     gene            31918..33282
                     /gene="mnmE"
                     /locus_tag="NBI74_000880"
     CDS             31918..33282
                     /gene="mnmE"
                     /locus_tag="NBI74_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HCD1931253.1"
                     /translation="MSDNDTIVAQATPPGRGGVGILRISGLKAREVAETVLGKLPKPR
                     YADYLPFKDADGSVLDQGIALWFPGPNSFTGEDVLELQGHGGPVILDLLLKRILTIPG
                     LRIARPGEFSERAFLNDKLDLAQAEAIADLIDASSEQAARSALNSLQGAFSARVNHLV
                     EALTHLRIYVEAAIDFPDEEIDFLSDGKIEAQLNDVIADLDAVRAEARQGSLLREGMK
                     VVIAGRPNAGKSSLLNALAGREAAIVTDIAGTTRDVLREHIHIDGMPLHIIDTAGLRE
                     ASDEVERIGIERAWQEIEQADRVLFMVDGTTTDAVDPAEIWPEFIARLPAKLPITVVR
                     NKADITGETLGMSEVNGHALIRLSARTGEGVDVLRNHLKQSMGFDTNMEGGFLARRRH
                     LQALEQAAEHLQQGKAQLLGAWAGELLAEELRLAQQNLSEITGEFTSDDLLGRIFSSF
                     CIGK"
     gene            33524..33598
                     /gene="tnaC"
                     /locus_tag="NBI74_000881"
     CDS             33524..33598
                     /gene="tnaC"
                     /locus_tag="NBI74_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase leader peptide"
                     /protein_id="HCD1931254.1"
                     /translation="MNILHICVTSKWFNIDNKIVDHRP"
     gene            33819..35234
                     /gene="tnaA"
                     /locus_tag="NBI74_000882"
     CDS             33819..35234
                     /gene="tnaA"
                     /locus_tag="NBI74_000882"
                     /EC_number="4.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709490.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HCD1931255.1"
                     /translation="MENFKHLPEPFRIRVIEPVKRTTRAYREEAIIKSGMNPFLLDSE
                     DVFIDLLTDSGTGAVTQSMQAAMMRGDEAYSGSRSYYALAESVKNIFGYQYTIPTHQG
                     RGAEQIYIPVLIKKREQEKGLDRSKMVAFSNYFFDTTQGHSQINGCTVRNVYIKEAFD
                     TGVRYDFKGNFDLEGLERGIEEVGPNNVPYIVATITSNSAGGQPVSLANLKAMYSIAK
                     KYDIPVVMDSARFAENAYFIKQREAEYKDWTIEQITRETYKYADMLAMSAKKDAMVPM
                     GGLLCMKDNSFFDVYTECRTLCVVQEGFPTYGGLEGGAMERLAVGLYDGMNLDWLAYR
                     IAQVQYLVDGLEEIGVVCQQAGGHAAFVDAGKLLPHIPADQFPAQALACELYKVAGIR
                     AVEIGSFLLGRDPKTGKQLPCPAELLRLTIPRATYTQTHMDFIIEAFKHVKENAANIK
                     GLTFTYEPKVLRHFTAKLKEV"
     gene            35326..36573
                     /gene="tnaB"
                     /locus_tag="NBI74_000883"
     CDS             35326..36573
                     /gene="tnaB"
                     /locus_tag="NBI74_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity tryptophan permease TnaB"
                     /protein_id="HCD1931256.1"
                     /translation="MTDQSEKKHSAFWGVMVIAGTVIGGGMFALPVDLAGAWFFWGAF
                     ILIIAWFSMLHSGLLLLEANLNYPVGSSFNTITKDLIGNTWNIISGITVAFVLYILTY
                     AYISANGAIISETISMNLGYHANPRIVGICTAIFVASVLWISSLAASRITSLFLGLKI
                     ISFVIVFGSFFFQVDYSILRDSTSTTAGTSYFPYIFMALPVCLASFGFHGNIPSLIIC
                     YGKRKDKLIKSVVFGSLLALVIYLFWLYCTMGNIPRESFKAIISSGGNVDSLVKSFLG
                     TKQHGIIEFCLLVFSNLAVASSFFGVTLGLFDYLADLFKIDNSHAGRFKTVLLTFLPP
                     ALLYLIFPNGFIYGIGGAGLCATIWAVIIPAVLAIKARKKFPNQMFTVWGGNLIPAIV
                     ILFGITVILCWFGNVFNVLPKFG"
     gene            36705..37880
                     /gene="mdtL"
                     /locus_tag="NBI74_000884"
     CDS             36705..37880
                     /gene="mdtL"
                     /locus_tag="NBI74_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtL"
                     /protein_id="HCD1931257.1"
                     /translation="MSRFLICSFALVLLYPAGIDMYLVGLPRIAADLNASEAQLHIAF
                     SVYLAGMAAAMLFAGKVADRSGRKPVAIPGAALFIIASVFCSLAETSALFLAGRFLQG
                     LGAGCCYVVAFAILRDTLDDRRRAKVLSLLNGITCIIPVLAPVLGHLIMLKFPWQSLF
                     WTMATMGIAVLMLSLFILKETRPAAPAASDKPRENSESLLNRFFLSRVVITTLSVSVI
                     LTFVNTSPVLLMEIMGFERGEYATIMALTAGVSMTVSFSTPFALGIFKPRTLMITSQV
                     LFLAAGITLAVSPSHAVSLFGITLICAGFSVGFGVAMSQALGPFSLRAGVASSTLGIA
                     QVCGSSLWIWLAAVVGIGAWNMLIGILIACSIVSLLLIMFVAPGRPVAAHEEIHHNA"
     gene            37855..38814
                     /gene="yidZ"
                     /locus_tag="NBI74_000885"
     CDS             37855..38814
                     /gene="yidZ"
                     /locus_tag="NBI74_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator YidZ"
                     /protein_id="HCD1931258.1"
                     /translation="MKKSITTLDLNLLLCLQLLMQERSVTKAAKRMNVTPSSVSKSLA
                     KLRAWFDDPLFVNSPLGLSPTPLMVSMEQNLAEWMQMSNQLLDKPLHETPRGLKFELA
                     AESPLMMIMLNALSKRIYQRYPQATIKLRNWDYDSLDAITRGEVDIGFSGRESHPRSR
                     ELLSSLPLSIDYEVLFSDVPCVWLRKDHPALHEAWNLDTFLRYPHISICWEQSDTWAL
                     DNVLQELGRERTIAMSLPEFEQSLFMAAQPDNLLLATAPRYCQYYNQLHQLPLVALPL
                     PFDESQQKKLEVPFTLLWHKRNSRNPKIVWLRETIKNLYASMA"
     gene            38971..39720
                     /locus_tag="NBI74_000886"
     CDS             38971..39720
                     /locus_tag="NBI74_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HCD1931259.1"
                     /translation="MATHFARGILTEGHLISVRLPSQCHQEARNIPPHRQSRFLASRG
                     LLAELMFMLYGIGELPEIVTLPKGKPVFSDKNLPSFSISYAGNMVGVALTTEGECGLD
                     MELQRATRGFHSPHAPDNHTFSSNESLWISKQNDPNEARAQLITLRRSVLKLTGDVLN
                     DDPRDLQLLPIAGRLKCAHVNHVEALCDAEDVLVWSVAVTPTIEKLSVWELDGKHGWK
                     SLPDIHSRANNPTSRMMRFAQLSTVKAFSPN"
     gene            39742..40308
                     /gene="yieF"
                     /locus_tag="NBI74_000887"
                     /gene_synonym="chrR"
     CDS             39742..40308
                     /gene="yieF"
                     /locus_tag="NBI74_000887"
                     /gene_synonym="chrR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I chromate reductase YieF"
                     /protein_id="HCD1931260.1"
                     /translation="MSEKLQVVTLLGSLRKGSFNGMVARTLPKIAPASMEVNALPSIA
                     DIPLYDADVQQEDGFPATVEALAEQIRQADGVVIVTPEYNYSVPGGLKNAIDWLSRLP
                     DQPLAGKPVLIQTSSMGVIGGARCQYHLRQILVFLDAMVMNKPEFMGGVIQNKVDPQT
                     GEVIDQGTLDHLTGQLTAFGEFIQRVKI"
     gene            complement(40361..41698)
                     /gene="adeP"
                     /locus_tag="NBI74_000888"
     CDS             complement(40361..41698)
                     /gene="adeP"
                     /locus_tag="NBI74_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeP"
                     /protein_id="HCD1931261.1"
                     /translation="MSQQHTTQASGQGMLERVFKLREHGTTARTEVIAGFTTFLTMVY
                     IVFVNPQILGVAGMDTSAVFVTTCLIAAFGSIMMGLFANLPVALAPAMGLNAFFAFVV
                     VQAMGLPWQVGMGAIFWGAIGLLLLTIFRVRYWMIANIPVSLRVGITSGIGLFIGMMG
                     LKNAGVIVANPETLVSIGNLTSHSVLLGILGFFIIAILASRNIHAAVLVSIVVTTLLG
                     WMLGDVHYNGIVSAPPSVMSVVGHVDLAGSFNLGLAGVIFSFMLVNLFDSSGTLIGVT
                     DKAGLADEKGKFPRMKQALYVDSISSVTGSFIGTSSVTAYIESSSGVSVGGRTGLTAV
                     VVGLLFLLVIFLSPLAGMVPGYAAAGALIYVGVLMTSSLARVNWQDLTESVPAFITAV
                     MMPFSFSITEGIALGFISYCVMKIGTGRLRDLSPCVIIVALLFILKIVFIDAH"
     gene            41865..42530
                     /gene="yieH"
                     /locus_tag="NBI74_000889"
     CDS             41865..42530
                     /gene="yieH"
                     /locus_tag="NBI74_000889"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconate phosphatase"
                     /protein_id="HCD1931262.1"
                     /translation="MSQIEAVFFDCDGTLVDSEVICSRAYVTMFREFGIHVDLEEIFT
                     RFKGVKLYEIINIISKEQGVTMIQADAEHVYRAEVARLFDSELEVIAGANELLASVKV
                     PMCVVSNGPVSKMQHSLGKLNMLHYFPEKLFSGYDIQRWKPDPALMFHAAKAMNVNVE
                     NCILVDDSSAGAQSGIDAGMEVFYFCADPHNKPIVHPKVTTFTHLSQLPELWKARGWD
                     ITA"
     gene            42597..43064
                     /locus_tag="NBI74_000890"
     CDS             42597..43064
                     /locus_tag="NBI74_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1931263.1"
                     /translation="MSVSRRVIHHGLYFAVLGPLIGVLFLVLYIFFAKEPLVLWVIIH
                     PIFLLLSITTGAIPALLTGVMVACLPEKIGSQKRYRCLAGGIGGVVITEIYCAVIIHI
                     KVMASSVLFENILSGDSLVVRIIPALLAGVVMSRIITRLPGLDISCPETDSLS"
     gene            43113..43700
                     /locus_tag="NBI74_000891"
     CDS             43113..43700
                     /locus_tag="NBI74_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CbrC family protein"
                     /protein_id="HCD1931264.1"
                     /translation="MTQNIRPLPLFKYHPKPLETGAFEQDKTVECDCCEQQTSVYYSG
                     PFYCVDEVEHLCPWCIANGSAAEKFAGSFQDDASIEGVEFEYDEEDDFAGIKNTYPDE
                     MLKELVERTPGYHGWQQEFWLAHCGDFCAFIGYVGWNDIKDRLDEFANLEEDCENFGI
                     RNSDLAKCLQKGGDCQGYLFRCLHCGKLRLWGDFS"
     gene            complement(43761..44483)
                     /locus_tag="NBI74_000892"
     CDS             complement(43761..44483)
                     /locus_tag="NBI74_000892"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HCD1931265.1"
                     /translation="MKLIITEDYQEMSRVAAHHLLGYMSKMRRVNLAITAGSTPKGMY
                     EYLITLVKGKPWYDNCYFYNFDEIPFRGKEGEGVTITNLRNLFFTPAGIKEENIQKLT
                     IDNYREHDQKLAREGGLDLVVLGLGVDGHFCGNLPNTTHFHEQTVEFPIQGEMVDIVA
                     HGELGGDFSLVPDSYVTMGPKSIMAAKNLLIIVSGAGKAQALKNVLQGPVTEDVPASV
                     LQLHPSLMVIADKAAAAELALG"
     gene            complement(44498..45667)
                     /locus_tag="NBI74_000893"
     CDS             complement(44498..45667)
                     /locus_tag="NBI74_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HCD1931266.1"
                     /translation="MNIKIAALTLAIASGISAQWAIAADMPASPAPTIPVKQYVTQVN
                     TDNSVTFRYFAPGAKNVSVVVGVPVPDNIHPMTKDEAGVWSWRTPVLKGNLYEYFFNV
                     DGVRSIDTGTAMTKPQRQVNSSMILVPGSYLDTRSVVHGDLIAITYHSNALQSERQMY
                     VWTPPGYTGIGEPLPVLYFYHGFGDTGRSAIDQGRIPQIMDNLLAEGKIKPMLVVIPD
                     TETDAKGIIPEDFVPQERRKVFYPLNAKAADRELMNDIIPLISKRFNVRKDADGRALA
                     GLSQGGYQALVSGINHLESFGWLATFSGVTTTTVPDEGVAARLNDPAAINQQLRNFTV
                     VVGDKDVVTGKDIAGLKTELEQKKIKFDYQEYPGLNHEMDVWRPAYAAFVQKLFK"
     gene            complement(45694..47310)
                     /gene="bglH"
                     /locus_tag="NBI74_000894"
     CDS             complement(45694..47310)
                     /gene="bglH"
                     /locus_tag="NBI74_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-specific porin BglH"
                     /protein_id="HCD1931267.1"
                     /translation="MFRRNIITSAILLMAPLAFSAQSLAESLTVEQRLELLEKALRET
                     QSELKKYKDEEKKKYTPATVNRSVSANDQGYAANPFPTSRAAKPDAVLVKNEEKNASE
                     TGSIYSSMTLKDFSKFVKDEIGFSYNGYYRSGWGTASHGSPKSWAIGSLGRFGNEYSG
                     WFDLQLKQRVYNENGKRVDAIVMMDGNVGQQYSTGWFGDNAGGENFMQFSDMYVTTKG
                     FLPFAPEADFWVGKHGAPKIEIQMLDWKTQRTDAAAGVGLENWKVGPGKIDIALVRED
                     VDDYDRSLQNKQQINTNTIDLRYKDIPLWDKATLMVSGRYVSANESASEKDNQDNNGY
                     YDWKDTWMFGTSLTQKFDKGGFNEFSFLVANNSIASNFGRYAGASPFTTFNGRYYGDH
                     TGGTAVRLTSQGEAYIGDHFIVANAIVYSFGNDIYSYETGAHSDFESIRAVVRPAYIW
                     DQYNQTGVELGYFTQQNKDANSNKYNESGYKTTLFHTFKVNTSMLTSRPEIRFYATYI
                     KALENELDGFTFEDNKDDQFAVGAQAEIWW"
     gene            complement(47396..48790)
                     /gene="bglB"
                     /locus_tag="NBI74_000895"
     CDS             complement(47396..48790)
                     /gene="bglB"
                     /locus_tag="NBI74_000895"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase BglB"
                     /protein_id="HCD1931268.1"
                     /translation="MKAFPETFLWGGATAANQVEGAWQEDGKGITTSDLQPHGVMGKM
                     EPRILGKENIKDVAIDFYHRYPEDIALFAEMGFTCLRISIAWARIFPQGDEAEPNEAG
                     LAFYERLFDEMAQAGIKPLVTLSHYEMPYGLVKNYGGWANRAVIDHFEHYARTVFTRY
                     QHKVVLWLTFNEINMSLHAPFTGVGLAEESGEAEVYQAIHHQLVASARAVKACHSLIP
                     EAKIGNMLLGGLVYPLTCQPQDMLQAMEENRRWMFFGDVQARGQYPGYMQRFFRDHNI
                     TIEMTESDAEDLKHTVDFISFSYYMTGCVSHDESINKNAQGNILNMIPNPHLKSSEWG
                     WQIDPVGLRILLNTLWDRYQKPLFIVENGLGAKDSVEVDGSIQDDYRIAYLNDHLVQV
                     NEAIADGVDIMGYTSWGPIDLVSASHSQMSKRYGFIYVDRDDNGEGSLTRTRKKSFGW
                     YAEVIKTRGLSLKK"
     gene            complement(48809..50683)
                     /gene="bglF"
                     /locus_tag="NBI74_000896"
     CDS             complement(48809..50683)
                     /gene="bglF"
                     /locus_tag="NBI74_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIABC"
                     /protein_id="HCD1931269.1"
                     /translation="MTELARTIVAGVGGADNIVSLMHCATRLRFKLKDESKAQAEVLK
                     KTPGIIMVVESGGQFQVVIGNHVADVFLAVNSVAGLGEKAQQAPENDDKGNLLNRFVY
                     VISGIFTPLIGLMAATGILKGMLALALTFQWTTEQSGTYLILFSASDALFWFFPIILG
                     YTAGKRFSGNPFTAMVIGGALVHPLILTAFENGQKVDALGLDFLGIPVTLLNYSSSVI
                     PIIFSAWLCSILERRLNAWLPSAIKNFFTPLLCLMVITPITFLLVGPLSTWISELIAA
                     GYLWLYQAVPAFAGAVMGGFWQIFVMFGLHWGLIPLIINNFTVLGYDTMIPLLMPAIM
                     AQVGAALGVFLCERDAQKKVVAGSAALTGLFGITEPAVYGVNLPRKYPFVIACISGAL
                     GATIIGYAQTKVYSFGLPGIFTFMQTIPSTGIDFTVWASVIGGVIAIGCAFVGTVMLH
                     FITAKRQPAQVAQQEKTPEIITPEQGGICSPMTGDIVPLIHVADTTFASGLLGKGIAI
                     LPSVGEVRSPVAGRIASLFATLHAIGIESDDGVEILIHVGIDTVKLDGKFFSAHVNVG
                     DKVNTGDRLISFDIPAIREAGFDLTTPVLISNSDDFTDVLPHGTVQINAGEPLLSIIR
                     "
     gene            complement(50816..51652)
                     /gene="bglG"
                     /locus_tag="NBI74_000897"
     CDS             complement(50816..51652)
                     /gene="bglG"
                     /locus_tag="NBI74_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional antiterminator BglG"
                     /protein_id="HCD1931270.1"
                     /translation="MNMQITKILNNNVVVVIDDQQREKVVMGRGIGFQKRPGERINSS
                     GIEKEYALSSHELNGRLSELLSHMPLEVMATCDRIISLAQERLGKLQDSIYISLTDHC
                     QFAIKRFQQNVLLPNPLLWDIQRLYPKEFQLGEEALTIIDKRLGVQLPKDEVGFIAMH
                     LVSAQMSGNMEDVAGVTQLMREMLQLIKFQFSLNYQEESLSYQRLVTHLKFLSWRIIE
                     HASINDSDESLQQAVKQNYPQAWQCAERIAIFIGLQYQRKISPAEIMFLAINIERVRK
                     EH"
BASE COUNT        12748 a        13524 c        12806 g        12774 t
ORIGIN      
        1 ctacccattc tacaaacata ctaagcctga atataaagag tacgactggg aacaaattca
       61 aggtatagct ttttttgcgc aaaatcacca ttccacatac gccaccattc gctgtattgt
      121 ttatgaatca ttagacagta caaatattat tgacaccttt aacctgataa cgacagagag
      181 tgctacaaat gcattgcata tgacaaataa caaattatgg ttatggatta atagctttat
      241 gaattttgac gatgagtttt tagagaaatc tatcaacact gctccaggta tttggggccg
      301 tgaagtgaat tggcctgatg atatgaataa gaaatcaatg ttgtcttcaa tggacgaata
      361 tatactctga ataatttgag ttgcagaata taagctcgcc cggatgtaaa tatcccatcc
      421 aggcgagaaa atcacttact gaaaaaacac cgcaatcttg ttaaacatcg tcgggtcgga
      481 ctgattacgc tgcactttca ccacatcttc cagcttgtcg atttggctta tcatctgctc
      541 cagacgctgg tcgtcattga ccagtagcca gatgtggctt ttgtcgctgt cctgaatcgg
      601 caggcagaga atgccttcaa cgttaaaagc gcggcgggca aaaaggccac aaacgtgggt
      661 cattacaccc ggatgattgc gaacggtgag ctccagaatc acgttatcat gagttgtgtt
      721 ttgcatggct tattccccca ccatttcagt attcgccgca cctggcggca ccatcgggta
      781 cactttttct tcggcatcaa tgcgcacatg gatcagcgcc gggccagggc gattgatgat
      841 ttcctgcaat gcagcctgcg gatcggcttc gttattcaaa tcacaggttt cgaggccgaa
      901 tccggcggca atctgcataa agttgatttt gcccggatag gtagcggcaa aaacgccctg
      961 cttgtagaac agactctgtt gctgatgcac cagccccagc gcttcgttgt tcatcaaaat
     1021 gattttaaca tccagctgat tttcactggc ggtcgccatc tcctgaatat tcatcatcag
     1081 gctgccgtcg ccggagaagc acaacacttt gcggtccggg ttcgccagcg ccgcgccaat
     1141 cgccgcaggc aggccaaaac ccatcgtgcc cagcccgccg gaggtcagcc actggcgtgg
     1201 gcgattgagc ggataggctt gcgcggtcca catctgatgc tgacccacgt cggtagtgat
     1261 aatcgcattg tcatcgacac aggcggcaac ggcgttgatc aggccgtaat ggcttaatgg
     1321 atcgcacgct ttcgggattg gacacgggaa ttcacgctgc aaatccgcca ccaactggtg
     1381 ccactctgca cgcggttgcg cttccaccag cgggatcaac tgcgccagca cgtcatcaac
     1441 atccgcctga attgccacat gcggttgctt gattttgccc agctctgcgc ggtcgatatc
     1501 gacatgaata attttggcat tcggacagaa ctgctctgtt ttgccaatcg cccggtcatc
     1561 aaaacgcgca ccgagtacaa tcagcagatc cgcctcctgc aaaatatagt tggtgctgcg
     1621 cacaccgtgc atccccagca tacccagcga cagcggatgc gcttttggca acatacccag
     1681 cgccattaaa gtcatggtgg taggcagttg cgctttctcc gccagttcac gcactcgcgc
     1741 aggcgcgttg atcacaccac cacccagata aagcaccggg tgtttggcgg cgttaatcat
     1801 cgccgccgcg tcacgaatac tttcttcgct aaaggcgggt gcggcggctt tttctgccat
     1861 agcgggctgt gtttcaatct caaaaactgc cgtttgcaca tccttaggaa tgtctatcca
     1921 caccgggcca gggcggcctg attgcgcaat gctgaaggca tcgctcatga cctgcgggag
     1981 ttcttcgata tgtctgacca gatagttgtg tttggtgatg gggatagaga tgccgtaggt
     2041 gtcgacttcc tggaaggcgt cggtgccgat catcgaggcg gaaacctgac cagtgatgca
     2101 aatcagcggg atggagtcca gcctcgcatc ggcaatggcg gtcaccaggt tagtcgcacc
     2161 cggtccgcta caggccatac agaccgccgg tttaccgtcg gtgcgcgcca ttccctgggc
     2221 gataaacccc gccccttgtt cgtggcgagc cagaatatgg cggatttgcg tgctttggct
     2281 taaggcatcg taaacaggca ggatagaacc gcccggaata cccgtcacaa tcttaatgcc
     2341 ctgctgttcc aggaaatgaa cgataaattc tgcgccggta aagcgcttac gcgtcgatgt
     2401 tgtgcccgaa cttgccatgc tccagtcctt ttcttctggg ccgactttcc gggaggtgct
     2461 taaacgaaaa accccgcccg gtttgcgccg gcggggtttt ggaatcgtgt gttgttccag
     2521 tccctacggc gcattgccga cgaccaccac cacacgcacg acgaccactg cggcggatgg
     2581 cgcagttggt agtagttttg cgttgaacat ggaagtagtc attagggacc ttgttggttt
     2641 tgtgtttaac aatatttata caagcacagc tttacagggg agacaatgga aaatttttct
     2701 tccggataaa aatacgtggc agatcacatt ttgaactgaa actcaggcaa taaagcatca
     2761 tcacctgaaa gacggtcagg ttgttgacag agtgcgcctt attcatgtcg aatacagcgt
     2821 aaacacctta ctctaccaac cgcaccacca ctgtttccgg caacatttat tgccgctttt
     2881 gtttacatat tctgccgcta aacaattccc cattcctggc gtatatctgg ctaacattca
     2941 tcaatgtgat agattcctct cccgcattta tgggaatgcg tagtgactta ttctaattat
     3001 ttttataaaa gcatccgtga taatgaaaag gcatagaaac gtcaatttgt tattgatgtt
     3061 ggtattactc gtggccgtcg gtcagatggc gcaaaccatt tatattccag ctattgccga
     3121 tatggcgcgc gatctcaatg tccgtgaagg ggcggtgcag agcgtaatgg gcgcttatct
     3181 gctgacttac ggtgtctcac agctgtttta tggcccgatt tccgaccgcg tgggtcgccg
     3241 accggtgatc ctcgtcggaa tgtccatttt tatgctggca acgctggtcg cggtctcgac
     3301 ctccagtttg atggtattga ttgccgccag cgcgatgcag gggatgggca ccggcgttgg
     3361 cggcgtaatg gcgcgtactt tgccgcgcga tttatatgaa cggacacagt tgcgccacgc
     3421 taacagcctg ttaaacatgg ggattcttgt cagtccgctg ctcgcaccgc taatcggtgg
     3481 tctgctggat acgatgtgga actggcgcgc ctgttatctc tttttgttgg ttctttgtgc
     3541 cggtgtgact ttcagtatgg cccgctggat gccggaaacg cgtccggtcg acgcaccgcg
     3601 cacgcgcctg cttaccagtt ataaaacgct tttcggtaac agcggtttta actgctattt
     3661 gctgatgctg attggcggtc tggccggaat tgccgccttt gaagcctgct ccggcgtgct
     3721 gatgggcgcg gtgttagggc tgagcagtat gacggtcagt attttgttta ttctgccgat
     3781 tccggcggca ttttttggcg catggtttgc cggacgtccc aataaacgct tctcaacgtt
     3841 gatgtggcag tcggttatct gttgcctgct ggctggcttg ctgatgtgga tccccgactg
     3901 gtttggcgtg atgaatgtct ggacgctgct cattcccgcc gcgctgttct ttttcggtgc
     3961 cgggatgctg tttccactgg ctaccagcgg cgcgatggag ccgttcccct tcctggcggg
     4021 caccgctggc gcgctggtcg gcggtctgca aaacattggt tccggcgtgc tggcgtcgct
     4081 ctctgcgatg ttgccgcaaa ccggtcaggg cagcctggga ttgttgatga ccttaatggg
     4141 attgttgatc gtgctgtgct ggctaccgct ggcgacgcgg atgtcgcatc aggggcagcc
     4201 tgtttaagct cacgtcaccg cagcatcgtc atcagctcca tgggagaacg atgctgcttt
     4261 atcagatcac gcatcacccg catatgcggc gcggagtaag aataaaacgc ctgatagccc
     4321 gcacaaagca cgttgtctgg tttatcttcc cgagcgtgcg ggcacaggcg atgccagtcg
     4381 cacgcctggc agtcacggcg cggttgcgac ttttcgctgc cacaccattt ttgtaaggtg
     4441 gtgtcaaaaa gttcaatcga aatacgactg atatctgccc gcacccaaca ctcgaacagg
     4501 gcaattaaaa atttccccca cagctctgct gtcaccgaag cggccgtcag atcgccccgg
     4561 tcatcaaatt caagatgggg aataagctgt actgagcgga tgcccagctg tttgaaattg
     4621 tgataaaaat cggcaggttg cagagcatcc ttatactcta caaccagttt atgcctgtcc
     4681 tcttcagcat caatgacctg acttcctgtc atgcgactct ctcaataaat acgataagtt
     4741 gatgtatatg cgttacagca aacagtattg cgagagataa caccgcgagg gagatcaaaa
     4801 atttgtcacg gactacgtga aattgggaaa aatcgctatt cgtgacatcc attaaattgc
     4861 gattacgggt gtagtgccag aggatcagcg ccacaatggc gaggatgcca atcgaaatcc
     4921 agaataacat gcccgcctgg tgccagttgt gtttgatagc caacgccatc aacgcgccgt
     4981 agcccagcat ggtacgaaac cacgccagtg atgtgcgttc cggctgcagc cctgggtcgg
     5041 caatgcgtct ggctttgcgg ctatccggca tacaacacca gtcccatgac gatcactgcc
     5101 acgaccatca atattaagct gatgatgaat aagctgttgg tatagggcag gtcctctttc
     5161 aggcgcatcg ctttttcatt gcgtaaccag cgcaagtagc catacatcgc taacccgccg
     5221 gaaaacagac acaacaacaa cgccagcagt tcgcggatga cgggcgtggc gaaatctggc
     5281 gcaagttgat caagcccaac ccccgccgcc agaaatccta gtgcagtacg gatccacgcc
     5341 agaaaagtac gctcattagc cagcgagaag cggtaatcgg gcgcttctcc gaggcgggaa
     5401 attttcattg ttgctccttg taaactgttc ctcgccgaaa aattaggcca ggaacagcaa
     5461 taatgcatca gaacttctgt tttttctctt ccaccttcac cccttgtgtc ggtaaaccgg
     5521 tgtcatagtc gcgtaccact ggagaataac catcctgtgg gcgtggacga aacgccccca
     5581 tccagcgcgg ttgtgcatct ttgcgccacg gacgcaggtt ccattgataa ctgcggaacg
     5641 gatcgcgaat tttgtccatg taatccaata aggcgtcatg cattttgctg cgaacgtcgg
     5701 caaaatggat atcatcgatc aggttatgca tttcatttgg gtcattacgg cgatcgtaaa
     5761 gttcatcact ggtgaagagg ttgagcacca gtttaaagtc atccgttacc cagcaacgca
     5821 ccggaataaa accgccaaag ctgtcatgct caatctcgta gcgattaaat tccaccaaca
     5881 cgccacgtgg ttcttttacg gcaaggatat tttcccccgg cagaatctct ggtttttcaa
     5941 tatctgccag cgccatcatt gtcggcagta agtcaatatg actgactggc gtatcgacct
     6001 gtcgccgctc cccttgcggc gaacggatga tcagcggaat gcgggtgatg tcgtcataca
     6061 tcgccgctcc tttactgatc agcttatgtg cgcccatcat ttcgccgtga tcggaggtat
     6121 aaataaccca cgtattttca cgttgctctg gcgttaaggc gttgatgacc cgtccgattt
     6181 ggtcatcaac aaagtcattg caggcaaaat agagcggatg gtgataaagc ccgtcatcac
     6241 cgactggcga tggcatcgcc tgcgcccata agcggtgatg ttccggtttg ttcgccaggt
     6301 catcctctgc tttctcaccc agttcgtagt aaaaatcagt gtatttctct aaatactcca
     6361 ccggacaggt gaacgggtga tgcggctcat catacgaaat caccatcagg aaaggttcgt
     6421 cggcgcgcgc gggctgttgc agaaaatcca ccgcccgatt gctgatgcgg tgcgcccagg
     6481 tgaaggtttc gtcaatatgg ttcgcctgta aatcttcgac gctgtttagg ccattgcgcc
     6541 acaggctaat ctctttttcc gtcagttcac taagatagtt cgccccatcg aaccagtaat
     6601 cagcgtccca ctccggcgga cactcgccag tgccgaaata gtcatgaccg tcgagatgcc
     6661 atttgccgat gtaacaggtg tgatagccgg catccttaaa gtagcgcccc atagtggaga
     6721 tgtttttgcc tggcgcgacg ttgttggtcc acgggccgga ctggttagcg tagataccgg
     6781 tgaatagtcc ggcgcgtgca ggcgtacaaa ccggtgaaca ggtgtaggcg gagttaaagc
     6841 gaataccttc cgccgccaga ctatcaatat tttgcgtatt cagcggctta ccgctatagc
     6901 aaccgaccat attggtggcc tgggtatcgg tcatgatgaa cagaaaattg gggcgtttca
     6961 taggctatcc tttgcatcgg gagtaaaggt tacggctggg tcgtgacgat gccgccagct
     7021 gtcaaaaatc aggtagacaa tcactacggc ggcaatgaaa taactgatca tcgctaacca
     7081 gcgcgtaccg tagccgccga attcagccag cccggcatag acgccaatca tcgcgaacag
     7141 gataccaatt gacgcagtct tgacgttttt ccacggtttc atgtccaccg caaacgcatc
     7201 tttgaaggtg aacggcgtgg cgcgcggttt gataaaaccg atcaccagca tcacgaccac
     7261 gttgatgcag aacgtacagg ccagcacata gaggaaatgg aagtcgaact tcaccagata
     7321 gttgatggtg atgtagctga ttatgccaat ccccatcgcc acttttgccg ccagcgccgg
     7381 aatgcgcggg aagaaaaagc ccataatgat gatggtaacc agcggcacgt tgtaaatacc
     7441 gttgagctgt ttcatccagc tatacagccc ctgcggcgcg ttggcgatcc acggcgcgac
     7501 catcaccgaa acgatggcga taaagaaacc aaatttgcgc ccgacggtga ccagctgttg
     7561 cggctcggca ttctggttaa tgatacgccg gtaaataccc atactgaata acgtactggc
     7621 gctattcaga aagccgttga aggtactgat caccgcacca aataacaccg caccgaagaa
     7681 gcccaccagt ggcaccggca gaacgttatt gaccagcgtc gggtaggcca tgtcggcttt
     7741 cggtaaatcc tgatacagat gaaacgcaat caaccccggc agcaccagta ccagcgggtc
     7801 gagcattttc agcaccgccg ttaacagtgc ccccttttgc ccttccgcca ggctttttga
     7861 cgccagcgtg cgttgcacga tgccctgatt ggtacaccag taaaaggtgt taaccagaat
     7921 caaaccggta aatgccgcgc caatcggcaa gggatcggtc gggccaccga ctgagtttaa
     7981 tttctcggcg tgaacggtgg taagttgctc aatgccctgc ataaagctgc ccttgcccat
     8041 cgcgatcagg ccaaataccg gcaccatcaa cccgccaata accagcccaa taccattgat
     8101 ggagtccgcc actgccattg cgcgcagtcc gccgatcacc gcatacaaaa ttcccgccag
     8161 accaagcaaa ataaccagta accagatagc cgcaccatcg gaaagttgta gcgactcacc
     8221 cacatgaaac aggctgttga gcgccaacgc gccggaatag agaacaatcg gcagaaagca
     8281 gacgccggtg gcaatgagga agcagaagtc gatgataata cgcgtcgttt tatcataacg
     8341 ttcctccaga aaatcgggga tggtggcaat gccgcgcttc agatagcgcg gtaaaaagat
     8401 tagcgcgagg aagatcagcg tcactgcaga ggtgacttcc cagcccatca ccgacatgcc
     8461 gcttttgtag gcctgcccgg aaaggccgac cagttgttcc gtggaaaggt tggttagcat
     8521 taacgaagcg gcaataaccg gcgcttttag tgaacgtccg gcaagaaaat agccttgttg
     8581 cgatccggta tctgttttac ggaccttcca ccaggtgatg accgccacca gcagcgtaaa
     8641 accgacaaaa ctcaagattt gtaacgaatt catctcatag cccttattta tgtttatgtc
     8701 ccggacgcat gaatataggg agaggtaaaa tgcgtcgggt caggcagtca gattcttaaa
     8761 atacaaacgt cgtatccctg aacggattgt tttctgttaa gttcaggttg tgagcataat
     8821 tcgccggagg gatattaaaa tgaatggaaa attgcaaagt tcggatgtaa aaaacgaaac
     8881 tccgtacaat attcccttat tgattaacga aaatgtgatc tccagcggaa tttctctgat
     8941 atcgctctgg catacctacg ccgacgagca ttatcgggtg atctggccgc gggacaagaa
     9001 gaaaccgctt atcgccaact catgggttgc ggtgtatacc gtacaaggat gtgggaaaat
     9061 tcttttaaag aatgacgaac aaataactct gcatggcaac tgtattatat ttttaaagcc
     9121 aatggatatt cactcttatc actgtgaagg tttggtctgg gaacagtact ggatggaatt
     9181 tacccccacc agtatgatgg atattcccgt cggtcagcaa agcgttattt ataatggcga
     9241 agtttataat caggaactta ccgaagttgc tgagttaatt acttcaccag aagcaataaa
     9301 aaataatctg gcagtcgctt ttctgacgaa aattatttat cagtggattt gtcttatgca
     9361 cgcagtcggt aaaaaagatc cacaacggcg gcaaattgaa aaattaattg ccactttaca
     9421 tgccagtcta caacaacgct ggagcgtagc tgatatggct gccacgatcc cctgtagcga
     9481 ggcctggttg cgtcgtctgt ttttacgcta taccggcaag acgccgaaag aatattacct
     9541 cgatgcgcgt ctggatctgg cgctatcgct attaaagcaa caagggaact cggttggcga
     9601 agtcgctgat acgctcaact tcttcgactc ctttcatttc agcaaagcct ttaagcataa
     9661 atttggttat gcgccgtcag ctgtgctgaa gaatacggac aggtaataac cagaatggcg
     9721 agtgtgtaaa tgactcgatg atgttcagaa ataccaggcg gagtaccatt tacggcgact
     9781 ccaccgggaa aagagttaac cgatactcca gaagagcacc gccagtaatt ggggtgtgat
     9841 aatgcgcagg aacatcacta acggatagac agtggcgtaa gagagcgccg ctgcaccgct
     9901 ggttggatga agattattag caaacgccag cgccggagga tcggtcatgg aacctgccag
     9961 catcccgcac atggtcaggt aattcatttt ggctaacatc cgcgccagaa taccaacagt
    10021 aatcagcgga acggcggtga tcagggcacc ataaccaatc cagcttagcc cttcgccatc
    10081 caccagtgta tggataaaat cgccgccgga tttcaggcca acgaccgaga ggaacagtac
    10141 gatccccagc tcccgcagcg cgaggttggc gcttggcggc ataaaccagt acagcttgcc
    10201 gatactgccg atacgcccga gaatcaacgc cataatcagc ggaccgcccg ccagccccag
    10261 tttcaacgcg gccggaaatc ccggcacaaa gacgggaata gaacctaaca atacgccaag
    10321 cccgatacca ataaacactg gcagcatctg aacctgttgc agtttttgct gcgcattccc
    10381 cagcacattg gcaacggcat caattgcgga cggacgcccc accaggttga ggatatcgcc
    10441 gaactgcagg ctgatatcgc tacttgcaac taattcaaca ccagcacggt tcaggcgcga
    10501 gataacaacg tcatagcgtt ctttaaagtg caggtcgcga atacgtttac cgagcacgtt
    10561 ttcattggtg accaccacac gctcgacgcg caaatcagtg cctttcgtag acagcgaggt
    10621 atcgacctcc tgaccaatca ccagttgcgc attatgtaaa tccgcaggct gccccaccag
    10681 atgcagcaaa tcgcccagtt gaataacggt ttctggcgaa ggcactttta gggtttcttc
    10741 tcgtttcaat cgtgagcaaa taactttatc gccattgaga atcggcacat ctttaatggc
    10801 taaattatgc aaattaggat tctcaactcg gatgttaatc gtcctgatta gcgcaccgcc
    10861 gttggttcgc gtggactcgt gctgctgggc ctcagtctca acattgacgc ggaaaataac
    10921 ccgcagcatc cacatagtga acaaaatgcc gcaaatccca aatggatacg ccatcgcata
    10981 gctcatcccc atttgatcga ccatcgccat cggtgtcccc aggtcgcgca agatctgctg
    11041 ccctgccccc agcgctggcg tattggtgac cgcgccggaa aaaatcccca gcacgaccgg
    11101 cagtggaata tcaaacagtt tatgcaggat ggcggtaacc agaccaccga tgatgacgat
    11161 cagaacagca aacaggttga ggcgtaatcc ggagacgcgc aatgaggcaa agaagcccgg
    11221 ccctacctga atcccgatgg tataaacgaa caggatcagg ccaaattcct gaataacatg
    11281 cagcatatcg ctacttaatg tcatccccgc ctgagaaaca aaatggccga cgatgatccc
    11341 gccgaacagc acgccgccaa tgcccaatcc aacaccgcga aacttgacgt tgccgataaa
    11401 caacccgacg accgccacca aagccagaat actgaccgtt aatgctatat cactcatcgt
    11461 ccatttctct tgaaataaca ttttaattgc tacggattat ggctgaatcg gtgaagcaga
    11521 tttgccttat agcgcacatt atcacgggaa agaacgtgcc gaaatatctt aaacaggcga
    11581 aatttaacgc attgttgaag aaaaaagaca aaaaaaaagc cccaccagaa tggcggggca
    11641 aagagaatag ctagttagct atttaacgcg ggacgttcgc tgatagcgat acgctgcgct
    11701 gcgatgggtt caggctcatt acgaattaaa tcaatatgca gtaaaccgtt tacgaaggtt
    11761 gcgccagaga cttccatatt ttcagccagc gtaaagctca ggctaaaagg ctgattcata
    11821 agcccttgat gcagccattt tttctcttct tttggctgct ccggtgtgcc ttttacgctc
    11881 aggcgcgtac cttccagttg aatctctaaa tcttcctgac gaaaacctgc cagcgcgagg
    11941 gtaatgcggt agtggttatc gtcgcttttc tcaatgttgt acggcgggaa gctctggctt
    12001 tcaccggcgt tttgcagtgc gttggccagt ttgtcaaaac cgatccattg acgcatcagt
    12061 ggggataaat cgaagttacg catagtcatt tctccttcta agaagcgagt aagtacctgc
    12121 aaatccgaag attcgcatat gctccctgac ggcgagcatg gagatgtcag gccgcgccag
    12181 gcggccttag ggaattagtt gatttcgata cggcgcggtt ttttcgcttc cggaatcacg
    12241 cgttcgagat cgatatacag cagaccattc accaggttag caccacgaac atgaatgttc
    12301 tcagctaact ggaatttacg ttcaaagttg cgttcagcga tgccctggta cagataggtg
    12361 cgctcttttt gttcgtcggc gtgagcacct ttcaccacca gcagattatc ctgggcggta
    12421 atttccagtt cgctctcagc aaaaccagcc acagcgatag caatgcggta atggttttcg
    12481 tctaccagtt caacgttata cggagggtag ccgccattac tctggctctg gttgttttct
    12541 aagtggttaa acaaacggtc aaatccaata gcagaacggt aaagcgggga taaatcaaag
    12601 ttacgcataa tcaatagctc ctgaaatcag cgagaatcta agaccttcca caatggacag
    12661 gtcaggtagc cagaacaccc atcaggcgcg tcctcatcgg ctacgatgta aaaatgggtc
    12721 tggaaatgaa cttttcaagc cttatcagcg gacttttttt tgcagtttat ggtctattgc
    12781 atagactgag ggggcagcac gaaacgttaa agtgcgatag ccgccacagt gcgacgtaat
    12841 gatgatgtta ttttcagcaa gggatcgcta taactcatca ggccaacacc cacgatcaac
    12901 agatgaattt attatgataa aaaatgtgtt gttagcgttc atgatatgca gcggaatgac
    12961 attactcgga ggatgctcca gcgtgatgtc ccataccggg gggaaagaag gaacgtatcc
    13021 tggcacccgc gccagcgcaa ccatgattgg cgacgatgaa accaactggg gcacaaaatc
    13081 gctggcgata ctcgacatgc catttaccgc agtactggac acaatattgc taccgtggga
    13141 tgtgttccgc aaagacagct cagtgcgctc gcgcgttgaa aaaagcgaag ccgacgcgca
    13201 agaaaccaat aacgtcattc cccccgccaa aatgcctgca aactgagagc tagcggtcag
    13261 tttcggtgat ccacaactga ccgtcctcca tccacgccaa ccattgacca tccggcgaga
    13321 aaaccacggc gtccgcagaa ggtggattgg cgtggttttc ggttaaatac tcaacctcgc
    13381 cgctttgcgc atgggcacaa gcaatccgat tatccagcac aaatcccagc cattcgcctg
    13441 atggatgcca gttaaatgca gactgaatat ccgttttatt atgggttaac tggcgtggtt
    13501 caccgccctg tggcgagata agccacagtt gcacaatgcc gttatcatca cgcattaaaa
    13561 acgcgatttg cgtaccctgc ggattacagc gcacccagtg gcgcgggacg ttgactaatc
    13621 ccggagaagc ccgatggtgc gtaaaggtta aacgtcgctg cacgacacca cgcggtggcg
    13681 cgggcagggt tgtttccgtt ccacttaacg gcgcatcacc tgccactttc cagccagctt
    13741 catcttgcgg caactcgaca ataaacagct ccggcacttt ctcgcccttt ggcgaaagtg
    13801 tgtcgccaat aaacgccagc gcgtgatttc ctacccatcc ttcttcataa gcacgattga
    13861 tttcatcact gccaggctgt ggcgtgggcg tggttttact taccagcacg caccagtggc
    13921 taccgctgta ttcacgcgga tgctgctttt gtacgttgac cgggccaaac ggtgcagcaa
    13981 caccgacatt tcgcaaatcc agcgccggat cgagttcatg cattacatgg tcgttgtagg
    14041 taaagctcac cctttcaccg tttgggctaa agacatgtac atgactgccg ccgcgcagcg
    14101 ctcctggcgt atatggcgcg gtaatatcca ttgcatcgag attgctcacc ttgccgcttt
    14161 caacaatcac tccgcgccga tgatgaaagt cgtaatacca tgtttcatca ggattttccg
    14221 ggccgtggat gaagacatac ttctctgact tcggatgaac ggtcaccacg ccgacgtatg
    14281 cgccctgtga cgcgcgatag ataacctcga cctcgccggt atggatattc acacgctcaa
    14341 tggtctcgcc ggtaaacgac gccccagaag gccgcacgtc aaacaccagc cactggctgt
    14401 cgggcgtcca ggtattggta ttggtgagca ggtgattacg gggagcaaag gttatctgtt
    14461 tcatggcgcg gtcctgatac aaaacggttc ccgccatcat acttcacaag gacttcactt
    14521 tcagacaata gtcttttgcc atcgtgatga atgtgagtga aaagatggaa catttcgacg
    14581 tggcgattat tggcctcggc ccggcagggt cggcgttggc gcgaaagtta gcaggcaaaa
    14641 tgcaggtgat cgcgctggat aaaaagcacc agtgtggtac tgaaggtttc agcaaacctt
    14701 gtggcggtct tctggcaccg gacgcgcagc gatcttttat tcgcgatgga ctgacgcttc
    14761 ctgtcgatgt catcgccaac ccgcagattt tcagcgtcaa aaccgtcgac gtcgccgcat
    14821 cgctcacgcg taactaccag cgaagctata tcaatattaa tcgccatgct ttcgacttgt
    14881 ggatgaaatc gctgatcccc gccagcgttg aggtttacca cgacagcctg tgtcgaaaaa
    14941 tctggcgtga ggatgataaa tggcatgtca tttttcgcgc agacggctgg gagcagcata
    15001 ttaccgcccg ctatctggtc ggtgccgatg gtgccaactc gatggtgcgg cgacatctct
    15061 acccggatca tcagattcgt aaatatgtcg ccatccagca gtggttcgca gagaaacatc
    15121 cggtaccgtt ctactcctgc atctttgata atgcgataac tgactgttac tcatggagta
    15181 tcagcaaaga cggttatttt atctttggcg gtgcctatcc catgaaagac ggtcagacgc
    15241 gtttcacgac gctaaaagag aaaatgagcg cctttcagtt ccagtttggt aaggcggtga
    15301 aaagcgaaaa atgcacggtg ctgtttccct cacgctggca ggattttgtc tgcggcaagg
    15361 acaacgcctt tctgattggc gaagcggcgg gatttatcag cgccagctcg ctggagggga
    15421 ttagctatgc gctggatagc gcagagattc tgcgttcggt attactgaag cagccagaga
    15481 agctcaatac cgcttactgg cgcgccactc gcaaactgcg tttaaaactc ttcggcaaga
    15541 tagtaaaaag ccgatgcctg accgcaccgg ctttaagaaa gtggattatg cgcagtggag
    15601 tggcacatat tccacagttg aaagattatc caacgcgctt cacatcgccc accagcagga
    15661 tgtaagagag cgcgccaatc aacgcgacgg cggagatata aaccagtgca ggcgcgaaac
    15721 cgtaaccctg cgccaggtac cccaccacca gcggaacggt gatgccgccc agaccaccgg
    15781 caaagttaaa cacgccgccg gttaaaccaa tcaggcgcat cggtgccaga gaagagacca
    15841 gcgaccaggt tatcgaagca aaaccgttgc cgaagaatgc cagcgccatc aggcacataa
    15901 tcatcatcgg atcgttggtg tagttcgcac ccataatgca ggtggagatc agcaagccgc
    15961 agataatcgg cgttttacgc gcaaagccca gtgaaaagcc cttacgtacc agcagatccg
    16021 cgacccagcc agagagcagg acgccaataa acgccgcgag gaatggcacc gtggtcataa
    16081 aacctgcttt cagcgccgtg attccttttt cctgggttaa atagttcggg aaccaggtta
    16141 agaaaaacca cagtgtagaa gccaccgcaa attgcccaag atagacaccg atcagtttac
    16201 gatggaacac cagtttccag tctttggctg ttaacggctg acgcgcctct tttttcaccg
    16261 gcgcatcgcc atccaccaga ccgccgccat cacgaatgta atccagttca gctttgctga
    16321 tgcctttggt cagacgcggc ggctgataaa ccttaaacca aatcagcgac cagataatgc
    16381 cgataccacc ggtgacaatg aacacccagt gccagctcaa catctcctga atccagatca
    16441 gcagcggagt cagaaacgcc agaccgacaa actgaccaga cgtataaaaa ccaacggcag
    16501 aagcgcgttc atgttccggg aaccaactgg ttaccatccg gttattggtc gggaacgcgg
    16561 gcgcttcgaa aataccggtt atcgcgcgca gaccaattaa tgacattaag cccgtggcaa
    16621 agccctggaa taacgtcgcc actgaccagc caaatatcgc aataaaataa gtcacgcgtg
    16681 aacctacgcg atctaaaaac caaccgccgg ggatctgaca tagcgtataa agccaggcga
    16741 aggccgaaaa tacatagccc atttgcgctt tagtaatacc aaactcttcc tgaatatggg
    16801 cagaagccac ggccaggtta gcacggtcaa cataacaaat gaccaccgta ataaagatca
    16861 tcaccagcgt cagataacgc cgacgccccg gctttgctgc attaacggga atatccatag
    16921 cgagctttct cctgattttg ggcatagcga agccgctcac catgcactgt aatttacaga
    16981 gggttatatt tttgtattgc tgttttagtg cccgatgagg ggcttacgtg gcaggaatta
    17041 ccactctgct acgctgttat cttcatgacg ccagagcgga ttacgccagt ccggggcatt
    17101 tttactgaac tcaataactt tagcttcgtc gatttccacg cctaagcccg gtttcgttaa
    17161 tggtttaaag aaaccgccga ccatgctgaa gtcctctttg tttttcacaa agtcgagtaa
    17221 ctccgcgcct ttgttgtaat gaatgcccat actttgttcc tgaaggacgg cgttatagga
    17281 aacaaagtcg atatgcaggc aagccgccag tgcaatcggt ccgagcggac agtgcggcgc
    17341 aagggtcacg tcataggctt ctgccattcc ggcaattttg tagcattcgg taataccgcc
    17401 tgcgtgggag agatccggtt gcagaatcga aataccgcct gcctccagca cacgtttaaa
    17461 atcgaagcgt gagaacatgc gctcacccgc cgccagtgga atatgcgttt gtgccgccag
    17521 tttcgggtag tattcggcct gttccgccag caccggctct tcaataaaca gcggacgata
    17581 cggttccagc tctttaatca gcactttcgc catcggcgcg ctgacgcgac catggaagtc
    17641 cagaccaaac tcaatctgat tgccaaaagc ttcacgaatt tgtgccacgg tgttgactgc
    17701 cgcatctacc gcgcgggagt tatcaattag ccccagttct tcacaaccgt tcagtttgaa
    17761 ggtatcgaag ccgatttcgc gtagcgtttt aatgccgtcg ataacatccg ccggacgatc
    17821 gccgccgacc caactgtagg ctttaatttt gtcgcgaacc aggccgccca tcagttgcca
    17881 gaccggcgca ttcagcactt tgcctttgat atcccataaa gcctggtcga tcccagcgat
    17941 ggcactcatc agaatcggac cgccacgata aaatccggcg cgatacatca cttgccataa
    18001 gtcgttgatg cgtgacgggt cctgaccaat caaatagtca ctcagctcgt ggactgccgc
    18061 ttccaccgtg cgagcgcgac cttcaatcac gggctcgccc caaccgacca cgccttcatc
    18121 ggtttcaatt ttcaggaaca tccagcgggg aggtaaacga tacgtggtaa ttttggtgat
    18181 tttcattgca ctgcctctcg atacgcctta acaaatgctg ctgcctgctg cgcagtccgt
    18241 tctacggact gcccggcgcg atagagatcg ctacctaagc ccgctccggc acagcctgca
    18301 tctatccact gcgccaggtt ttctggcgtc acaccgccaa cggcaaagac cgcgatgttc
    18361 ggtggtaata ccgcttttaa tgctttgatg tattgcggac caaaagccga tgacggaaat
    18421 attttcagtg cctgcgcacc cgcgtcgagc gcggtaaagg cttcggtcgc cgtcgcgcag
    18481 ccggggcaga cggtcatgcc gtagcccacc gcacggcgga tcacttcact atggatattg
    18541 ggcgtaacga tgagctgaca gcccattctg gcgagcgcat cgacctgttc aggtttcagt
    18601 accgtacctg cgccaatcaa cgccttgtcg ccatacgcat caacgatggc gggaatgctc
    18661 tgctcccatt gtggagaatt cagcgggatt tcaaccgcgt cgaacccggc gtcaatcacc
    18721 gcgccaacat gcgccagcgc ctcgtcgggc gtaatgccgc gcaaaatggc gatcagcggg
    18781 agtttagttt gccactgcat gagcgatgtt ccttatacca gcctgaaatg ccgtgtcgcc
    18841 cgccaccgcc gtcacgtcac aacccatcgc ctgaaaggct tgctggtagc gcgcggtcag
    18901 cgatgttccg gcgacaaggg tgatgacttg ttgatgggcc acatagtcgc gcatactggc
    18961 gacctctgcg ccaatcaaca aaccagagag aaattcgctg acctgttcgc ggggaagtgt
    19021 tcccagcaca tgcgaggcgc gaacttcaaa aagctgtggc aatacggcgg gcgcattaag
    19081 gccacgctca agaccagccg cgaaagcatc gacagcgttt tcctgcggcg gcaaacctgc
    19141 gccaatcaat gagtgattta acagtaaatg atgtaattca ccggtcatca cggtgcgaaa
    19201 atcgttaatt tgctggctat cggcctgcac ccatttgcaa tgcgtaccgg gcatgacata
    19261 aagagaggaa ggagcgagag tgcgcgcgcc gatcaattgt gtttcttcgc cacgcatcac
    19321 attgtggtta tcgtcatgag agacgcataa tccgggaata atccagatat tgtcgccaac
    19381 agacgttaat tgttcgccaa tagacgaaaa acgggcagga atggataaat acggcgcaac
    19441 tttccagccg acattgctgc cgaccattcc tgccattacc actggcgtat tctcttcacg
    19501 ccagtcggtc gtcacttctg ctaacaccgc agccggagat tttccattca ggcgcgtgac
    19561 gcctgcttct gattgcctgc tgtccaggca gtggtcgccc tgataaagcc aggcgcgcag
    19621 attggtcgat ccccagtcaa ttgcgatgta gcgagctgtc atgtgatttc ctttaacctt
    19681 cgtgtcgagc tggcgatcat ggtaagcgcc gcctgctctg ccgcatcgcc gtcctgatgc
    19741 cttatcgcat cgaacagcgc cttatgttcc tggagcgttt gcggcatgtt ggcctcatcg
    19801 cccatccagg ttcgttcaaa aaccgcccgc tgcagcgaac tgatcgcaat gctcagttgt
    19861 tgtaacaccg ggttatgcac cgactgcagc accgcctcgt ggtagcgaat atccgcttcg
    19921 ttaaacgctt cgcggtcctg attgttagca atcatctcgt tcagcgccga ttcaatctgc
    19981 gccagatcgc tggaggtcgc gcgctctgct gcccaacggg caatagccgg ctctaccagg
    20041 tttcgcactt cgctcattgc actgataagc cgtgggtcat agtcgttttc cagcacccat
    20101 tgcagtacgt cagtgtcgag gtaattccac tggttacgcg gagccacaaa cgccccgcga
    20161 taacgtttca tttcaatcag ccgcttcgcc atcagcgaac gaaatacctc acggatgatg
    20221 ttgcgcgagg ttgcaaactc ctcacagagt tctgcctcag ccggaagcgg cgagcctggc
    20281 acgtatttgc cgtgaacgat ctgtttaccc agcgtaataa cgatgcgatc ggttttattg
    20341 agagtcatgg agagtccttg tgctctgtat gttcttctct actttacccc gatcgatgca
    20401 taacgcggca attttgtagt accagcgtga tgacgttcgc gtttgctgtg cgcataatgt
    20461 agtacaaatt tatattgttg tactacaatt tagatcacaa aaagaacaac ccataagaaa
    20521 atgttctacg tcgggcaaaa atctgaaaag ggagatcagg cgctaaacag gagggaaaga
    20581 agagtatgct ttcaacggct tagcactctt gtttaaagga ttaatcatga aattgaattt
    20641 taaaggattt tttaaggctg ccggtttgtg ttctttggca tttgcgttga ccggatgtat
    20701 cacctgggga ttggtttcca atactgcaag ccgtccacat gccgagcaat ggcggtcaga
    20761 cacgattaag ggtttatcgc tggctgaaga cagtaacggt acgaaaggct atgtttttgt
    20821 tggtgaatcg ttggactatc tattgaccac cggcggcgat gaggtggtta acatgctgaa
    20881 cgatccggca atccatggtg aaagaatcac cgtttctgat aacgcgaaat ttattttaag
    20941 cagcagtaat aagaatttct ctggtgctat tacgctctat tacgactgga acaacgaaga
    21001 agataaggca ctggcaacac aatatggttt tatttgtgat acccggcgtt gtacatggat
    21061 gctggacggt ttgaaaggct caatccacca gaaaaataaa aaagcggatt actcaaacgt
    21121 gatggttttc catcaaccct ttacggtggg attttatgag tacaaagcaa cagatggcgt
    21181 cccgcgcggc ctcgttaatg cgctgctgcc agtcactctt acgctggata tagtaacgtc
    21241 accactccag ttcctgattt tatgcacgac cagaaattgt taatcgctgc gggcaaacgc
    21301 attcatgccg tttgcccgtt cactgattaa ttcagcacat acttctcaat agcaaatgcc
    21361 acgccatctt caaggttaga tttggtgaca aagttcgcca cttctttcac cgaaggaata
    21421 gcgttatcca tcgccacacc gacgcctgca tattcaatca ttgcgatgtc gttttcctga
    21481 tcgccaatcg ccatgatttc ttccggttta atacctaaca cgtcggccag tgatttcaca
    21541 cccgtacctt tgttaacgcg tttatcgagg atttcgagga agtacggcgc acttttcagc
    21601 acggtgtatt tttctttcac ttcctgcgga atacgcgcaa tggcctggtc gaggatggcg
    21661 ggttcatcaa tcatcatcac tttcaggaac tgggtattgg ggtccatttt ctccgcttcg
    21721 cagaacacca gcggaatggt ggcaacgaaa gattcatgca ccgtgtagta gctgatatct
    21781 cggttggcgg tgtacagcgt ggtgcggtcc agggcgtgga aatgagaacc gacttcgcga
    21841 gagagttttt ccaggaaacg gtagtcgtca tagctgagag cggtttgcgc cacggtgcta
    21901 ccatcagcgg ccttctgtac cagcgcgccg ttataagtaa tgcagtagtc gcccggctgt
    21961 tccatatgca gctctttcag gtaattgtga acacctgcat aagggcgacc cgtcgttagc
    22021 acgacattca cgccacgggc gcgagctgcg gcaatcgcat ttttaacggc gggtgaaatg
    22081 gtgtgatcgg gcagcagaag ggtgccatcc atatcgatag caatgagttt gatagccatg
    22141 agttccccag gtagattggt tcctgaccca tgctaacgcg attccgctca aaaatcagta
    22201 caacacccga gggaaaaggg gatgcaacgc gcattgcgtg ctccccattt gcttagcgga
    22261 agagtttccc tttcagcagt tccatacctg cggaaagcag atcgttattg gcttgtggtg
    22321 acacttcacc ttgcggtgag agtgcatcaa taatcttcgg caattgttct gccagtaaac
    22381 tggaagctgt actggtatcc acgccaagtt tttgcccgag atcggacacc gcatttgtgc
    22441 cgagcgccga ttccacttgc tcgccgctaa ctgattgatt gctctgttga ttactcagcc
    22501 aggttgagag aatggcccct aagccgccac tttgcagttt ttccagcagc acctgaatgc
    22561 cgccctgctc ctcaacccag ctcaaaatcg cctgatattt ccccgcatcg cctttcagaa
    22621 aggcaccgac aacttcatca aaaagcccca tgataatcac ctgtaaagcg ttacgtgttg
    22681 acccaaaaag tatagatctg cgggatgata attgcggatt gcaggaataa aaagggcgga
    22741 gatgatctcc gcccggactg acgactaagt cattttatca gcaacaaaaa gccattaaat
    22801 atcgatattc gccgctttca gagcgttctc ttcgataaac gcacggcgcg gttcaacggc
    22861 gtcgcccata agcgtagtga acaactggtc ggcagcaatc gcatctttaa cggtaacgcg
    22921 cagcatacga cggctttccg ggtccatggt ggtttcccac agctgttccg ggttcatctc
    22981 gcccagacct ttataacgct ggatggagag gccgcgacgg gactctttca ccagccagtc
    23041 cagcgcctgc tcgaagctgg ctaccggctg acgacgttcg ccacgttcga taaatgcatc
    23101 ttcttccagc aagccacgca gtttctcacc cagcgtgcag atacgacgat attcgccgcc
    23161 agtgataaat tcgtgatcca gcggatagtc agtatccaca ccgtgggtac gcacgcgaac
    23221 aatcggctcg aacaggtttt gttcggcatt ggtatggaca tcaaacttcc actggctgcc
    23281 gtgctgttct ttatcgttca gttcgctgac cagcgcgttc acccagcggg taacggtttg
    23341 ctcgtcagaa aggtcggttt ccgtcagcgt cggctgatag ataagctctt tcagcattgc
    23401 tttcggataa cgacgctcca tgcggttgat cattttctgc gtcgcgttgt actcagacac
    23461 cagtttctct aacgcttcgc cagccagcgc cggtgcactg gcgttggtgt gcagcgttgc
    23521 gccatccagc gcgatagaga tctggtactg atccatcgct tcgtcgtctt taatgtactg
    23581 ttcctgctta cctttcttca ctttgtacag cggcggctga gcgatgtaga cgtgaccacg
    23641 ttcaacgatt tccggcatct gacgatagaa gaaggtcaac agcagcgtac gaatgtgcga
    23701 gccgtcgacg tccgcatcgg tcatgatgat gatgctgtga taacgcagtt tgtccgggtt
    23761 gtactcgtca cgaccgatac cacaaccaag cgcggtgata agcgtcgcca cttcctgaga
    23821 agagagcatc ttatcgaagc gcgctttctc gacgttgagg attttaccct tcagcggcag
    23881 aatcgcctgg ttcttgcggt tacgcccctg cttcgcagag ccgcccgcgg agtccccttc
    23941 cactaggtat agttcggaaa gcgctgggtc gcgttcctgg cagtctgcca gtttgcccgg
    24001 caggcccgct aagtcgagcg cacctttacg gcgagtcatt tcacgcgcgc gacgcgccgc
    24061 ttcacgggca cgggcagcat cgataatttt gccaaccacg attttcgcgt cggttgggtt
    24121 ttccagcagg tattccgcca gcagttcgtt catctgctgt tcaaccgccg atttcacctc
    24181 agaagaaacc agtttgtctt tggtctgtga ggagaatttc gggtccggca ctttcacgga
    24241 aacgaccgca atcaggcctt cacgcgcatc gtcaccggtg gcgctgactt tggctttttt
    24301 gctgtagcct tctttgtcca tgtaggcgtt cagagtacgg gtcatcgccg cacggaaacc
    24361 tgccaggtga gtaccgccgt cacgctgcgg aatgttgttg gtaaagcagt agatgttttc
    24421 ctggaagcca tcgttccact gcaacgccac ttcgacgcca ataccgtctt tttcggtgga
    24481 aaagtagaag atattcgggt ggatcggcgt tttgttcttg ttcagatatt caacgaacgc
    24541 cttgatgccg ccttcatagt ggaagtggtc ttctttgccg tcgcgcttat cgcgcagacg
    24601 aatggaaacg ccggagttga ggaacgacaa ctcacgcaga cgtttcgcca gaatttcata
    24661 ttcgaactcg gtcacattgg tgaaggtttc aaggcttggc cagaaacgca ccatggtgcc
    24721 ggttttttca gtctcgccgg taaccgccag cggggcctgc ggtacaccgt gttcgtagat
    24781 ctgacggtga attttaccct cgcgctggat aaccagctcc agtttttgcg acagggcgtt
    24841 tactaccgaa acaccaacgc cgtgcagacc gccggacact ttataggagt tatcgtcaaa
    24901 tttaccgcct gcgtgcagaa cggtcatgat cacttccgcc gccgatacgc cctcttccgg
    24961 gtgaataccg gtcggaatgc cgcgcccgtc atcctgtaca gagacagagt tatcggcgtg
    25021 aatggtgacg ataatttctt tacagtgacc cgcgagcgct tcgtcgatag cgttatctac
    25081 cacctcgaat accatgtggt gcagaccggt gccgtcatcc gtgtcgccga tatacatacc
    25141 cgggcgctta cgcaccgcat ccagcccttt caggactttg atactggagg agtcataaga
    25201 attcgacatc aacgtttctc gctcatttat tcttgggtta atccgttatt ttaccctttt
    25261 ccacggtaaa catcttcgaa ttttcgtccg acatgtctat tacgtgttca gcactgatcg
    25321 cgctgacaaa gacctgtgat tgcgtcgctt ttaagcggct ggcaagcagc ccgcgacgct
    25381 catcatcaag ctcagaggca aaatcatcta tcaggtagag acaccgccgc ccgctttcac
    25441 gggtgaggaa ctctccttgc gccagacgta aggcgcacat caacagctta agctgtccac
    25501 gcgataaggt atcttccacc ggcgcaccgt cggcgcgaat gcgtaagtcc gctttatgcg
    25561 ggccgtgcgc ggtgtaggtt aactggcgat cgcgttcaaa attacgttcc agcacctcag
    25621 cgtactctgt ctctttctcc cagccgcgct ggaaagagaa agtcagagaa aactcaggga
    25681 gaaattgctt acaggtatca gccatatcgg ctgcgatacc ggcgctatac tccgcgcgcc
    25741 aggtgctgat ttgctccgcc agcgggatca gttctttatc ccacgggcgt agctgttcgt
    25801 aacgagtcac ctggcgcagc gccgcattgc gctgcttgag caatcgcttg agattgctcc
    25861 aggcggtgaa aaatccgggt tcgttgtgaa agcatcccca gtcgaggaat gctcttctgt
    25921 atttggggcc gccattgagt aaagtaaacc cttctggcgt tatcagctgc attggcatca
    25981 ggtgcgccag ctccgcgact ttatgcccgt ctgtaccgtc aatacggact ttgctgtcgc
    26041 cttgtttgtc tttggttaaa ccaatcgccg tctcgcgctc ttcgccctgt aatcgtccgt
    26101 ggagaacaaa cgcctcctgc tcgtggcgaa tgacgcgacc aatctgcaaa ctgcgaaacg
    26161 cccgaccgtg gccgagcgta tagatggctt ccagcacgct ggttttgcca ctgccgttgg
    26221 cacctaccag aaagttaaag ccgggagata aggcgagatc cgcggtttca atgttgcgga
    26281 aatcgcggat caacaagcgg gtgagggaca ttacagtctc attggcatga caacataagc
    26341 cgcgctctgg ctggccgcat cttcaatctg cacgctggaa accgaatcgg tcagcatcat
    26401 gcggacgttt tcgcatttca gtgcgttcag aacatccagc acatagctga cgttgaagcc
    26461 gatttccatc tccgcaccgc tataggtaac gtcgaggatc tcttccgctt cttcctgttc
    26521 tgggttgttg gcggtgattt tcagctggtt ttcgctgaca tacagacgca cgccgcggaa
    26581 tttctcgtta gagagaattg ccgcacgggc aaacgcctgc ttgagcagat cacagccagc
    26641 ttccagatgc ttgtccgggt tcttcggcag aacgcgacgg taatccggga agcgaccatc
    26701 caccagtttc gaggtgaaga taaagtcgcc aacgtgggcg cgaatgttgt tgctgccaat
    26761 ctgcacgcgc agcggattgt cgccgccgtc gagcatacgc atcagttcaa ttacgccttt
    26821 acgcggcacg atcaccgaat ggcttggcaa agattgacca attggcattg aacagactgc
    26881 cagacggtgg ccgtcggtcg ccacggtgcg cagttcttca ccttcggttt caaacagcat
    26941 accgtttaag taatagcgaa cgtcctgatg tgccatagaa aactgggtcg cttcaatcag
    27001 acgcttcatc gtcgcctgcg gcagggtaaa ttcgacttcg ctctgccagt catcgaggtt
    27061 cgggaaatct gccgctggca gggtagacag cgaaaaacgg ctacgcccgg agcgcaccag
    27121 catccgctca ccttccagct gcacggcaat ttccgcgcct tcaggcagac cacggcagat
    27181 atcaaagaat ttgcgcgccg gaacggtcgt cgcccctggt tcgtgcggct gaaccagcgc
    27241 aacgcgtgcc accatttcca tctcgagatc agtaccggtc agcgacaacg taccgtcagc
    27301 aacctgtaac agcagattac cgagaatcgg tagcgtagga cgaccaccta acggaccgct
    27361 cacctgttgt agcggtttta ataaatgctc acgttctacg gtaaatttca taggtttacg
    27421 atgacaatgt tctgattaaa ttagaaaaat cttctttgat atcgtggctc tcttcacgca
    27481 actgctcgat cttccggcag gcatgaagca ccgtcgtgtg gtcacgacca ccaaaggcat
    27541 cgccaatctc cggcagactg tggttagtca gctctttcgc cagcgccatc gccatctggc
    27601 gcggacgggc caccgagcgg gatcgacgct tggaaaggag atccgcgact ttgatcttgt
    27661 agtactccgc caccgtcttc tgaatattgt cgatggtgac cagtttttcc tgcaatgcca
    27721 gcaagtcgcg cagcgcctca cgcacgaagt cgatggtgat cgcccgtccg gtaaagttgg
    27781 cgttggcaat gacgcggttc agcgcgcctt ccaactcacg tacgttagat cgtagacgct
    27841 tggcgataaa gaacgccact tcgcccggca aacgaatgtc gttttcgtcg gcctttttca
    27901 tcaggatcgc cacgcgggtt tccagctctg gcggttcgat cgccacagtc agtccccagc
    27961 cgaagcggga tttcaaacga tcctcaacgc cgttgatctc tttcggatag cgatcggagg
    28021 tgagaatgat ctgttgatta ccttccagca gggcgttgaa ggtgtggaaa aactcttcct
    28081 gagatcgttc tttattagca aaaaactgaa tatcgtcgat cagcagtgca tctacggaac
    28141 ggtagtagcg tttaaactct tcgatcgcgt tgttttgcag ggctttaacc atgtcctgaa
    28201 caaagcgctc ggagtgcata taaaccactt tggcattcgg cttgcgcgcc ataatgccgt
    28261 tacccaccgc atgcagcagg tgagttttac ccaaacccgt gccgccataa aggaacaacg
    28321 ggttataagc accgccaggg ttatctgcca cctggcgagc cgccgcgcgc gccagttggt
    28381 tagatttacc ttcaacgaag ttatcaaacg tgtgtttgac gtttacgtta gaacgatagg
    28441 tcggttctgc cggagccggg acgttatccc aacccgagcg cgtagaaggc gcagcacgtt
    28501 gcggctgcgt ttgcgccacc tgtgcagggg ccgcgacgtt gctcgtcact gccgcttgtg
    28561 gcgtttgcgt caccggtttg gtgccgactt caaaacgcag ctgtggggca tccgctccgc
    28621 agaaactggt tagcagtcca ttgatattat taaggtactt gtcccgtacc caatcgagga
    28681 caaaacggtt tggcgcgtac agggccagcg tgttatcgct cagttccgcc tgcaatgggc
    28741 gtatccacat actgaattct gtggctggta actcatcctg caatcgggca agacactgct
    28801 gccaaagcga aagtgacacg gcggactcca ctcgaacaaa agtcgataat gactaaggct
    28861 gaaacattca tgattgttga cgtacgtcga aaagaccctg catgagggtg acgcacgaac
    28921 cgctgtctgc ggttatatgc ccgatcaaga tcctgcaaaa cgatcgggac cgcggatcat
    28981 agcctaaact gcgcaagaga tcttctgttt ctcacagatt ttcccgattt atccacagga
    29041 tgatccagaa ctcgctaagt gtaaacgatc ctgccgcagg ccgggcacga tttacgccgc
    29101 atattggaaa atttaatgac catagacaaa aaatggctta atcgatctaa taaagatcca
    29161 ggacgatcct tgcgctttac ccgtcagccc gtataatcct ccacccggcg cgccatgctg
    29221 gttttcactg gtgtgaggcc gtacgttttc cccgcgaaaa ggtgcggaaa agcgcggtaa
    29281 ataaggaaag agaattgact ccggagtgta caattattac aatccggcct ctttaatcac
    29341 ccatggcttc ggtgtccatc gtttcatttt tcggcggata tccaataaag ccattgaatt
    29401 tattcaagtt taggtagaaa tcgccatgaa acgcactttt caaccgtctg tactgaagcg
    29461 caaccgttct cacggcttcc gtgctcgtat ggctactaaa aatggtcgtc aggttctggc
    29521 acgtcgtcgt gctaaaggcc gcgctcgtct gaccgtttct aagtaataaa gctaacccct
    29581 gagtggttaa gctcgcattt cccagggagt tacgcttgtt aactcccagt caattcacat
    29641 tcgtcttcca gcagccacaa cgggctggca cgccgcaaat tactattctc ggccgcctga
    29701 attcgctggg gcatccccgt atcggtctta cagtcgccaa gaaaaacgtt cgacgcgccc
    29761 atgaacgcaa tcggattaaa cgtctgacgc gtgaaagctt ccgtctgcgc caacatgaac
    29821 tcccggctat ggatttcgtg gtggtggcga aaaaaggggt tgccgacctc gataaccgtg
    29881 ctctctcgga agcgttggaa aaattatggc gccgccactg tcgcctggct cgcgggtcct
    29941 gatagccctc attcgggtct atcaacgcct gattagtccg ctactcgggc cgcattgtcg
    30001 tttcactcca acctgttcaa gctacggaat tgaggcattg cgcaggtttg gagtgataaa
    30061 aggcagttgg ttgacggtga aacgcgtatt aaaatgccac cctttacacc ctggtggtga
    30121 cgatcccgtc ccgcccggac catttgatac cagagaacac taacgatgga ttcgcaacgc
    30181 aatcttttag tcatcgcttt gctgttcgtg tctttcatga tctggcaagc ctgggagcag
    30241 gataaaaacc cgcaacctca ggcccaacag accacgcaga caacgaccac cgcagcgggt
    30301 agcgccgccg accagggcgt accggccagt ggccagggga aactgatctc ggttaagacc
    30361 gacgtgcttg atctgaccat caacacccgt ggtggtgatg ttgagcaggc cctgctgccg
    30421 gcttacccga aagagctgaa ctctacccag ccgttccagc tgctggaaac ttcaccgcag
    30481 tttatttatc aggcacagag cggtctgacc ggtcgtgatg gcccggataa cccggctaac
    30541 ggcccgcgtc cgctatataa cgttgaaaaa gacgcttatg tgctggctga aggtcaaaac
    30601 gaactgcagg tgccgatgac gtataccgac gcggcaggca acacgtttac caaaacgttt
    30661 gtcctgaaac gtggtgatta cgctgtcaac gtcaactaca acgtgcagaa cgctggcgag
    30721 aaaccgctgg aaatctccac ctttggtcag ttgaagcaat ccatcactct gccaccgcat
    30781 ctcgataccg gaagcagcaa cttcgcactg cacaccttcc gtggtgcggc gtactccacg
    30841 cctgacgaga agtacgagaa atacaaattc gataccattg ccgataacga aaacctgaac
    30901 atctcttcga aaggtggttg ggtggcaatg ctgcaacagt atttcgcgac ggcgtggatc
    30961 ccgcataacg acggtaccaa caacttctac accgctaatc tgggtaacgg catcgccgct
    31021 atcggctata aatctcagcc ggtactggtt cagcctggtc agactggcgc gatgaacagc
    31081 accctgtggg ttggcccgga aattcaggac aaaatggcag ctgttgctcc gcacctggat
    31141 ctgaccgttg attacggttg gttgtggttc atctctcagc cactgttcaa actgctgaaa
    31201 tggatccata gctttgtggg taactggggc ttctccatta ttatcatcac ctttatcgtt
    31261 cgtggcatca tgtacccgct gaccaaagcg cagtacacct ccatggcgaa gatgcgtatg
    31321 ctgcagccga agattcaggc aatgcgtgag cgtctgggcg atgacaaaca gcgcataagc
    31381 caggaaatga tggcgctgta caaagctgag aaggttaacc cgctgggcgg ctgcttcccg
    31441 ctgctgatcc agatgccaat cttcctggcg ttgtactaca tgctgatggg ttccgttgaa
    31501 ctacgtcagg caccgtttgc actgtggatc cacgacctgt ctgcacagga cccgtactac
    31561 atcctgccga tcctgatggg cgtaacgatg ttcttcattc agaagatgtc gccgaccact
    31621 gtgaccgacc cgatgcagca gaagatcatg acctttatgc cggtcatctt cactgtgttc
    31681 ttcctgtggt tcccgtcagg tctggtgctg tactatatcg tcagcaacct ggtaaccatt
    31741 attcagcagc agctgattta ccgtggtctg gaaaaacgtg gcttgcatag ccgcgagaag
    31801 aaaaaatcct gattcggtga gttttcgcta aaataagggc ggtcagttga ccgccttttt
    31861 tcttttcgta gggcggataa gcaccacaca tccgccacac aaagcaacag gaacatcatg
    31921 agcgataatg acactatcgt agcccaggcc acgcctccgg gacgtggcgg cgttggcatc
    31981 ctgcgcatct ccggcctcaa agcccgtgaa gttgccgaaa ccgtgctggg taaactgcct
    32041 aagccacgct acgccgatta tcttccgttt aaagacgccg atggcagcgt gctcgatcag
    32101 gggattgcgc tatggttccc tggcccgaac tcgttcaccg gcgaagatgt gctggaactg
    32161 caaggtcatg gcggtccggt gatcctcgac ctgctgttaa aacgcattct gaccattccc
    32221 ggcctgcgga ttgcccgccc tggtgagttt tccgaacgcg catttctgaa cgataaactc
    32281 gacttagccc aggccgaggc gattgcggat cttatcgacg ccagctcgga acaggcggcc
    32341 cgttcggcgc taaactcgct gcaaggcgcg ttctccgcac gggtcaacca tctggtggaa
    32401 gccctcaccc acttgcgcat ttacgtcgaa gcggcaattg atttcccgga tgaagagatc
    32461 gatttcctct ccgacggcaa aattgaagcc cagctcaatg acgtaattgc cgatctcgac
    32521 gcggtacgcg ccgaagctcg tcagggtagt ttgttgcgcg aagggatgaa agtggtgatt
    32581 gccggacgtc ctaacgccgg taaatcgagc ctgctgaacg cgctggcggg ccgtgaagca
    32641 gcaatcgtaa ccgatatcgc cggaaccacg cgtgacgtgc tgcgtgagca tatccacatt
    32701 gacggaatgc cgttgcatat catcgatacc gccgggctac gtgaagccag tgacgaagtt
    32761 gaacgtattg gtatcgagcg cgcatggcag gaaattgaac aggcagaccg cgtgctgttt
    32821 atggtcgatg gcaccacgac agacgccgtt gatccggcag agatctggcc ggaatttatc
    32881 gcccgtctgc cagcgaaact gccgatcacc gtggtgcgca ataaagccga tatcaccggc
    32941 gaaacgttgg ggatgagcga agtgaacggt cacgcgttaa ttcgtctttc ggcgcggact
    33001 ggcgaaggcg tggatgtgct gcgtaaccat ctcaaacaga gcatgggctt tgacactaat
    33061 atggaaggcg gcttcctggc gcgtcgtcgt cacctacagg cgctggaaca ggcggcagag
    33121 catttgcagc aaggtaaagc gcaactgtta ggtgcctggg cgggtgaact gctggcggaa
    33181 gagttgcgcc tggcgcagca gaacttaagc gagatcaccg gggagtttac ttcagacgac
    33241 ctactggggc ggattttctc cagcttctgt atcggtaagt aaccgcgctt gcgaagccgc
    33301 attctgactg tcagatgcgg cttcgcttca ttgttagcac tcctgttatt cctcaaccct
    33361 tttttaaaca ttaaaattct tacgtaattt ataatcttta aaatagatat ttaatattgc
    33421 gtcccgaacg attgtgattc gattcacatt taaacaattt cagaatagac aaatttcttg
    33481 agtattatac tgtagcctcg tgtctttcga ggataagtgc attatgaata tcttacatat
    33541 atgtgtgacc tcaaaatggt ttaatattga caacaagatt gtcgatcacc gcccttgatt
    33601 tgcccttctg tagccatcac cagagccaaa ccgattagat tcaatgtgat cgattagttt
    33661 gctatatctt aattttgcct tttacaaagg ccatctctcg tttatttact tgttttagta
    33721 aatgatggtg cttgcatata tatctggcga attaatggat attgcaaatg taatattcac
    33781 agggatcact gtaattaaaa taaatgaagg attatgtaat ggaaaacttt aaacatctcc
    33841 ctgaaccgtt ccgcattcgt gttattgagc cagtaaaacg taccactcgc gcttatcgtg
    33901 aagaggcaat tattaaatcc ggtatgaacc cgttcctgct ggatagcgaa gatgtgttta
    33961 tcgatttact gaccgacagc ggcaccgggg cggtgacgca gagtatgcag gccgcgatga
    34021 tgcgtggcga cgaagcctac agcggcagcc gcagctacta cgcgttagcc gagtcagtga
    34081 aaaatatctt tggttatcaa tacactattc cgactcacca gggccgtggt gcagagcaaa
    34141 tctatattcc ggtactgatt aaaaaacgcg agcaggaaaa aggcctggat cgcagcaaaa
    34201 tggtggcgtt ctctaactat ttctttgata ccacgcaggg ccatagccag attaacggct
    34261 gtaccgtgcg taacgtctat atcaaagaag ccttcgatac tggcgtgcgt tacgacttta
    34321 aaggcaactt tgaccttgag ggattagaac gcggtattga agaagttggc ccgaataacg
    34381 tgccgtatat tgttgcaacc atcaccagta actccgcagg tggtcagccg gtttcactgg
    34441 caaacttaaa agcgatgtac agcatcgcga agaaatacga tattccggtg gtcatggact
    34501 ccgcacgctt tgccgaaaac gcctatttca tcaagcagcg tgaagcagaa tacaaagact
    34561 ggaccatcga gcagatcacc cgcgaaacct acaaatatgc cgatatgctg gcgatgtccg
    34621 ccaagaaaga tgcgatggtg ccgatgggcg gcctgctgtg catgaaagac aacagcttct
    34681 ttgatgtgta taccgaatgc agaacccttt gcgtggtgca ggaaggcttc ccgacatatg
    34741 gcggcctgga aggcggcgct atggagcgtc tggcggtagg tctgtatgac ggcatgaatc
    34801 tggactggct ggcttatcgt atcgcgcagg tgcagtatct ggtcgatggt ctggaagaga
    34861 ttggcgttgt ctgtcagcag gcgggcggtc acgcggcatt cgttgatgca ggtaaactgt
    34921 tgccgcatat cccggcagat cagttcccgg cacaggcgct ggcctgcgag ctgtataaag
    34981 tcgccggtat ccgtgcggta gaaattggct ctttcctgtt aggccgcgat ccgaaaaccg
    35041 gtaaacaact gccatgcccg gctgaactgc tgcgtttaac cattccgcgc gcaacatata
    35101 ctcaaacaca tatggacttc attattgaag cctttaaaca tgtgaaagag aacgcggcga
    35161 atattaaagg gttaaccttt acctacgaac cgaaagtatt gcgtcacttc accgcaaaac
    35221 tgaaagaagt ttaattaaaa cactcagagt ggctataagg atgttagcca ctctattacc
    35281 ctacatcctc aataacaaaa atagccttcc tctaaaggtg gcatcatgac tgatcaatca
    35341 gaaaaaaaac actctgcatt ttggggtgtt atggttatag ccggtacagt aattggcggt
    35401 gggatgtttg ctttacctgt tgaccttgcc ggagcctggt ttttttgggg cgcttttatt
    35461 cttattatcg cctggttttc aatgttacat tcagggctat tattattaga agcaaactta
    35521 aattatcccg tcggttccag ttttaacacc atcaccaaag atttaatcgg taacacctgg
    35581 aacataatca gcggtattac cgttgccttc gttctctata tcctcactta tgcctatatc
    35641 tctgctaatg gtgcgatcat tagtgaaacg atatcaatga atttgggcta tcacgctaat
    35701 ccacgtattg tcgggatctg cactgccatt ttcgttgcca gcgtattgtg gataagttca
    35761 ttagccgcca gtcgtattac ctcattgttc cttgggctga agattatctc ctttgtaatt
    35821 gtttttggtt ctttcttctt ccaggtcgat tactccattc tgcgcgattc caccagcacc
    35881 actgcgggga cgtcttactt cccgtatatc tttatggctt tgccggtgtg tctggcgtca
    35941 tttggtttcc acggcaatat tcccagcctg attatttgct atggcaaacg caaagataag
    36001 ctcatcaaaa gcgtggtctt cggttcgctg ctggcgctgg tgatttatct cttctggctc
    36061 tattgcacga tggggaatat cccgcgagaa agctttaagg cgataatctc ctcaggcggc
    36121 aacgttgatt cgctggtgaa atcgttcctc ggcaccaaac agcacggcat tatcgaattt
    36181 tgcctgctgg tgttctctaa cttagctgtt gccagctcgt tttttggtgt cacgctggga
    36241 ttgttcgatt atctggcgga tctgtttaag attgataact cccacgccgg gcgtttcaaa
    36301 accgtgctgt taaccttcct gccacccgcg ttgttgtatc tgatcttccc gaacggcttt
    36361 atttacggga tcggcggtgc cggactgtgc gccaccatct gggcggtaat aattcctgct
    36421 gtactggcaa tcaaagcccg caagaagttt cccaatcaga tgttcacggt ctggggcggc
    36481 aatcttattc cggcgattgt cattctcttt ggcataaccg taattttatg ctggttcggt
    36541 aacgtcttta acgtgttacc taaatttggc taaatctttc aggaagccag ccattcgctg
    36601 gcttcttgcc tgtcaggaaa tctcttttgt ccaaatggca actcgcctga ttatccttca
    36661 ccacgtatgc tttgcgtcac cttactatca ggacgcttta gcccatgtcc cgctttttga
    36721 tttgtagttt tgccctggtt ttactttatc ccgcagggat tgatatgtac ctcgttggtt
    36781 taccgcgcat cgccgccgat ctcaatgcca gcgaagcgca gttgcatatt gcgttctccg
    36841 tttatctggc ggggatggcg gctgcgatgt tatttgccgg taaagtggcc gatcgttcag
    36901 ggagaaagcc tgtcgccata cccggcgcgg cgctatttat tatcgcctcg gtgttctgtt
    36961 cactggctga aaccagcgcg ttatttcttg cagggcgatt tctacaggga ctgggcgcgg
    37021 gctgttgtta cgtagtggcg ttcgccattt tgcgcgacac gctggatgat cgacgtcggg
    37081 ctaaagtgct gtcgttactc aacggcatta cctgcatcat tccggtgtta gcgccggtgc
    37141 tcggacacct gattatgctt aaattccctt ggcagagtct gttctggacg atggcaacga
    37201 tgggcatcgc ggtactgatg ttgtctttgt ttattttaaa agaaacgcgc cctgcggccc
    37261 ccgcagcttc ggacaaacca cgagaaaata gcgagtcact gcttaaccgg tttttcctca
    37321 gccgtgttgt tatcaccacc ctcagcgttt cggtgatcct caccttcgtc aacacgtcgc
    37381 cagtattgct gatggaaatc atgggttttg agcgcggaga atacgccacc attatggcgt
    37441 tgaccgctgg cgtcagcatg accgtttcat tctccacgcc gtttgcgctg ggaattttta
    37501 agccacgtac gttgatgatc acctcacagg tgttattcct ggcggcgggg atcacccttg
    37561 ccgtttcacc ttcccatgcg gtttcgctgt ttggtatcac gctgatttgc gccggtttct
    37621 cggtaggttt tggcgtagcg atgagtcagg cgttggggcc gttttcatta cgcgcgggcg
    37681 tagccagctc gaccttaggt attgcgcaag tttgcggttc gtcactgtgg atttggctgg
    37741 cggcggtggt tggtatcggt gcatggaata tgctgatcgg gattctgatt gcctgtagca
    37801 tagtgagcct gttgctgatt atgttcgtcg cgcctggacg cccggttgcc gctcatgaag
    37861 aaatccatca caacgcttga tctcaatctg ctgctctgtc tgcaactgct gatgcaggag
    37921 cgcagcgtga ccaaagcggc gaagcggatg aacgtgaccc cttcgtcggt gagtaagtcg
    37981 ctggcaaagt taagagcgtg gtttgacgac ccgctctttg tgaactcacc gctggggctg
    38041 tcgcccacac cgctaatggt cagcatggag caaaatctgg cggagtggat gcaaatgagc
    38101 aatcagttgc tggacaaacc actccacgaa acgccgcgcg gtcttaagtt cgaactggcg
    38161 gcggaatcgc cgctgatgat gatcatgctt aatgcgctgt cgaaacggat ctaccaacga
    38221 tacccgcagg cgaccatcaa attacgtaac tgggattacg attccttaga tgccattact
    38281 cgtggtgaag tggatattgg tttttccggt cgcgaaagtc atccccgttc gcgggagctg
    38341 ttaagctcgc taccgttatc cattgattat gaagtgctgt ttagtgatgt gccctgcgtc
    38401 tggttacgca aagatcatcc ggcactgcat gaagcatgga atctggacac ctttttacgt
    38461 tatccgcata tcagcatttg ctgggaacag agcgatacct gggcgctgga caatgtgtta
    38521 caggagctgg ggcgcgaacg cacgattgct atgagcctgc cggaattcga gcagtcactg
    38581 tttatggcag cacagcccga caatctgcta ctggcgaccg cgccgcgcta ctgccagtac
    38641 tacaatcaac tccaccaact gccgttggtt gctcttcctc tcccgtttga tgaaagccag
    38701 caaaaaaagc tggaagtccc ttttaccctt ctgtggcata aacggaacag ccgtaatccg
    38761 aagatcgtct ggttacggga aaccattaaa aatctttacg cgtcgatggc ataaccgaat
    38821 cgtatgaaac gcgacccaat ttcacaatga aatgtaaaaa agttgtaata aatttatctg
    38881 aatcgaactt ttagccgctt tagtctgtcc atcattccag taaatgatta ctcttgtatt
    38941 cataatggac cattaagcat ggagcgaaaa atggcgactc actttgcccg agggatttta
    39001 acggaaggac atctgatttc tgttcgtctc ccctcccagt gtcatcaaga agcccgaaac
    39061 attccccctc atcgtcaaag ccgttttctg gcgtccagag gtttactcgc agaactgatg
    39121 ttcatgctgt atggcattgg cgaattgccg gaaatcgtca ccctgccgaa aggaaaaccg
    39181 gttttcagcg ataaaaattt gccttcgttt tctatttcct atgccgggaa tatggtcggc
    39241 gtggcgttaa caaccgaagg tgaatgtggc ctcgatatgg aactacagcg tgcgacacgc
    39301 gggtttcata gcccacacgc gcccgataac cacacctttt ccagcaatga atcgctatgg
    39361 atcagtaaac aaaacgatcc taacgaagcg cgggcgcagc tcatcacgct gcgccggagc
    39421 gtgctaaaac taaccggtga tgttttgaat gacgatccgc gcgatctgca actactgccc
    39481 attgccggac gcctgaaatg tgctcatgta aatcatgtag aagcgttatg cgacgcggag
    39541 gacgtgctgg tgtggtccgt ggcggtcacg cccacgattg aaaagctcag tgtctgggag
    39601 ttagatggca aacacggctg gaaaagcctg cccgatattc acagccgcgc caacaatcct
    39661 accagccgga tgatgcgttt tgcccagctc tctaccgtga aggctttttc gccaaattga
    39721 tagacaacca caggagtcat catgtctgaa aaattgcagg tggttacgtt actggggagc
    39781 ctgcgcaaag gctcatttaa tggcatggtt gcacgtaccc tgccgaaaat tgctccggcg
    39841 agtatggaag tcaatgcgtt gccttccatt gccgacattc ccctgtatga cgctgacgta
    39901 cagcaggaag acggttttcc agcaacggtt gaagctctgg cggaacagat ccgtcaggct
    39961 gacggtgtgg tgatcgtcac gccggaatat aactactcgg taccgggtgg gctgaaaaat
    40021 gccatcgact ggctttcccg cctgccggat caaccgctgg ctggtaaacc ggtattgatt
    40081 cagaccagct caatgggcgt gattggcggc gcgcgctgtc agtatcacct gcgccagatt
    40141 ctggttttcc tcgatgcaat ggtgatgaac aagccagaat ttatgggcgg cgtgattcag
    40201 aacaaagttg atccgcaaac cggggaagtg attgatcagg gtacgctgga ccacctgacc
    40261 gggcaattga ccgcatttgg tgagtttatt cagcgagtta agatctaaat aaaaacccgc
    40321 cagcaatcat gcatggcggg tttttaacgc gctatcgatt ttagtgagcg tcgataaaga
    40381 caatcttcag gataaacagc agcgcaacga tgattacgca cgggctaagg tcacgcagac
    40441 gtccggtacc aatcttcatc acgcagtagg agataaagcc cagcgcgata ccttcagtaa
    40501 tcgagaagct gaacggcatc atcacagcgg taataaacgc cggaacagat tcagtaagat
    40561 cctgccagtt cacgcgagca agacttgagg tcatcaacac gccaacgtaa atcagcgcac
    40621 cagctgcagc gtagcctggc accatccccg ccagcggcga caggaagata accagcagga
    40681 acagcagacc aacaaccact gccgtcagac cggtacgacc gccaacggaa acgccggaag
    40741 aagattcaat atacgccgta acagaagaag taccgataaa cgaaccggtc accgaagaga
    40801 tactgtcgac atacagcgcc tgcttcatgc gcgggaattt acctttctca tcagccagac
    40861 ctgctttatc ggtcacgcca atcagcgtac cggaggagtc aaacaagttg accagcatga
    40921 aagagaaaat cacccctgcc agcccaaggt taaacgaccc ggctaaatct acatgaccca
    40981 cgacactcat tacgctcggc ggcgcagaaa cgatgccatt atagtggaca tcacccagca
    41041 tccagcccag cagcgttgtc accacgatgg aaaccagcac cgccgcgtga atattgcgcg
    41101 aggccagaat ggcgatgatg aagaagccga ggatccccag cagcacgctg tgagaagtca
    41161 gattaccgat gctcaccagc gtttccgggt tagcgacaat cacacctgcg tttttcagcc
    41221 ccatcatgcc aataaacaga ccgataccac tggtgatgcc cacacgtaga cttaccggga
    41281 tgttggctat catccagtag cgaacgcgga aaatcgtcag taacagcaga ccaatcgcgc
    41341 cccagaagat tgcgcccatc ccgacttgcc acggcaagcc catcgcctgt acaacgacaa
    41401 aagcgaagaa cgcattcagg cccattgccg gtgccagtgc aactggcagg ttagcaaaca
    41461 gccccatcat aatactgccg aatgcagcaa tcagacaggt cgtgacgaag acggcgctgg
    41521 tatccatgcc agcaacgcca agaatttgcg ggttaacaaa aacgatgtaa accatcgtca
    41581 ggaaggtggt aaaaccggcg atcacttcgg tccgtgccgt cgtgccatgt tcgcgcagtt
    41641 taaacacgcg ttccagcatc ccctggccag aagcctgggt tgtgtgttgt tgactcatta
    41701 tctatttccg aacaaggagg gaaaatccgt cgctatcgta taccaaaatg agacaatatg
    41761 gacggctgaa gataactttt tttcattgta tttgcttata cggcaacgat tgcgtcatgc
    41821 gaattggcat gacgaaaacg ttaaactaac gataaaggaa agacatatcc cagatagaag
    41881 cggtattttt cgactgcgac ggtacgctgg tcgatagcga ggtcatttgc tctcgcgcct
    41941 atgtcacgat gttcagggaa tttggcattc acgttgacct ggaagagatt tttacccgtt
    42001 ttaaaggcgt aaaactctac gaaatcatca acatcatcag taaagaacag ggcgtgacga
    42061 tgatccaggc cgatgccgag cacgtttacc gtgcggaagt ggcgcgcctg tttgatagcg
    42121 agcttgaggt gattgcagga gcgaacgaat tgctggctag tgtgaaagtg ccgatgtgcg
    42181 tcgtctctaa cggcccggtc agcaagatgc agcattcgct tggtaagctc aacatgctgc
    42241 actacttccc ggaaaaactg ttcagcggct atgatatcca gcgttggaag cccgatccgg
    42301 cattaatgtt ccatgcggca aaagcgatga atgtgaacgt tgaaaactgc attctggtcg
    42361 atgactccag tgccggtgcg caatcaggga tagatgccgg aatggaagtg ttttacttct
    42421 gcgctgaccc gcacaataaa ccgatcgttc acccgaaagt caccaccttt acccatcttt
    42481 cgcaattacc ggaactgtgg aaggcgcgtg gttgggatat tacggcatag ttattcacac
    42541 tcccttcact tactccactt aaattggcac gcaaaggtaa gtaaagggag tttgatatgt
    42601 ctgtttcacg tcgggtaatt catcacggac tgtattttgc ggttttaggg ccgttaattg
    42661 gtgttctgtt tcttgtcctc tacatattct tcgcaaaaga accgctggtt ctttgggtga
    42721 taatacatcc aatttttctc ttattgtcga taactacggg agctattcct gcgttgttaa
    42781 ccggtgtaat ggttgcctgt ctgccggaaa agatcgggtc acagaaacgt tatcgttgtc
    42841 tggctggtgg cataggtgga gttgttatca ccgagatcta ttgcgctgtt atcatacata
    42901 ttaaggtcat ggcttcctca gtgttgtttg aaaacattct ttctggtgac agtctcgttg
    42961 tccgcatcat tcctgcattg ctggcaggtg tggtgatgag cagaatcatt acacgtctac
    43021 ccggactgga tatttcatgt cctgaaacag actctttaag ttaagcggga tgttttatct
    43081 tttggctact caaaagcaga caggatgttt ctatgactca aaatatcagg ccgttacccc
    43141 tattcaaata tcatcccaag ccactggaaa caggcgcatt tgaacaggat aaaaccgtag
    43201 agtgcgattg ctgtgaacaa cagacgtcag tttattactc gggtcccttt tattgcgttg
    43261 atgaagttga acatctctgt ccgtggtgta ttgcgaacgg ttctgctgct gaaaagtttg
    43321 caggtagttt tcaggatgat gccagcatcg aaggtgttga atttgagtat gatgaagagg
    43381 acgattttgc cggtattaag aacacatatc ctgatgaaat gctgaaagag ttggttgaac
    43441 gcacgccagg ttatcatgga tggcaacagg aattctggct cgcgcattgt ggcgatttct
    43501 gtgcttttat cggctatgtg ggctggaatg atataaaaga tcgcctcgat gaatttgcca
    43561 accttgaaga agattgtgag aatttcggta ttagaaattc tgatctagct aaatgcctgc
    43621 aaaagggtgg tgattgtcag ggttatctct tccgctgtct ccactgcggc aagctgagac
    43681 tgtggggcga tttttcatag ttatttatat gatgagaaca ggccggcgcg taattcacac
    43741 atccggcctt atttttaagc ttaaccgagc gctaattctg cggcagcggc tttatcagcg
    43801 attaccatca gcgaaggatg cagctgcaac acggaagcag gaacatcttc ggtaaccgga
    43861 ccttgcagaa catttttcag tgcctgcgct ttacccgcgc cactgacaat tatcagcaag
    43921 tttttcgccg ccatgatgct tttcggcccc atcgtcacgt agctgtcagg cactaatgaa
    43981 aagtcgccac ccaactcgcc atgcgccaca atatcaacca tctctccctg gatcgggaac
    44041 tccacggttt gctcatggaa atgggtcgta tttggcaaat tgccacaaaa atgaccatct
    44101 acacctaatc ccaacaccac caaatccagg ccaccttcac gcgccagttt ctgatcatgc
    44161 tcgcggtagt tatcaatagt gagcttctgg atattctctt ctttgatccc tgcaggggtg
    44221 aaaaacagat tacgcagatt ggtaatcgtt acgccttccc cttctttgcc acgaaatgga
    44281 atttcatcga aattgtagaa atagcagtta tcgtaccagg gcttaccttt aaccagagtg
    44341 atgagatatt cgtacatgcc tttgggcgtg ctaccggcgg taattgccag gttaacgcga
    44401 cgcatctttg acatataacc aagcagatga tgtgcggcga cacggctcat ttcctggtaa
    44461 tcttcggtaa tgatcaattt catcttaatg ccttatttta tttaaataat ttctgtacaa
    44521 aggctgcata ggctggtcgc cagacatcca tttcatggtt cagaccggga tattcctggt
    44581 aatcaaactt tatttttttc tgctcaagct cagttttcag cccggcgata tccttgccgg
    44641 ttacgacatc tttatcgccc acgaccacag taaaattacg tagttgctgg ttaatagctg
    44701 ccggatcgtt cagccgggca gcgacccctt catctggtac ggtcgtcgtg gtaacaccac
    44761 tgaatgtggc cagccagcca aagctttcca gatgattaat tcccgatacc agtgcctgat
    44821 acccaccttg tgaaagccct gccagtgcgc gaccatcggc atctttacgg acattaaaac
    44881 gcttgctaat cagcgggata atatcgttca tcagttcgcg atctgccgct ttagcattta
    44941 acggataaaa gactttacgt ctctcctgag gaacgaaatc ttcgggaata atgcccttcg
    45001 catcggtttc ggtatccggg atcaccacca gcatcggttt aattttccct tcagccagca
    45061 ggttatccat gatttgcggg atacgcccct gatcaatagc ggaacgtccg gtatcaccaa
    45121 agccgtgata gaaatagagc actggcaaag gctcgcctat gccggtgtat cctggcgggg
    45181 tccagacata catctgacgt tcggattgca atgcgttgga gtggtaagtt atggcgatca
    45241 aatcaccatg cacaacagaa cgtgtatcca gataactgcc cggtaccaga atcatactgg
    45301 agttaacctg acgctgaggc ttagtcattg ccgtgcctgt atcaatgctg cgtacaccat
    45361 caacattgaa aaaatactcg tacagattgc ctttcaggac aggcgtgcgc cacgaccaga
    45421 ctcccgcttc atctttggtc atcgggtgaa tattgtccgg aaccggaaca cccaccacta
    45481 cggagacatt ttttgcccca ggggcaaagt agcgaaaggt cacgctgtta tcggtattga
    45541 cctgagtcac atactgtttc accggaatag tgggtgccgg gctggctggc atatccgctg
    45601 ctatggccca ttgagcagaa ataccgctgg caatagccag cgttaaagca gcaattttta
    45661 tattcatcgt aatattcctt ttattaagtt ccattaccac cagatttcag cctgggcacc
    45721 gacagcaaac tggtcgtctt tattgtcttc aaaggtgaag ccatccagtt cgttttccag
    45781 agctttgata tatgtagcgt agaaacgaat ttccgggcgc gaggtcaaca tactggtatt
    45841 gactttaaag gtatggaaga gcgtggtttt ataaccagac tcattatatt tattgctatt
    45901 cgcatctttg ttttgctggg tgaaatagcc cagttcaaca cctgtctggt tatattggtc
    45961 ccaaatatag gccgggcgaa caaccgcacg aatagattcg aaatcagaat gtgcgcctgt
    46021 ttcgtagcta taaatatcgt taccgaagga gtaaacaatc gcgttagcca caatgaaatg
    46081 atcgccgata taagcttcgc cctgcgaagt cagacgaact gcagttccgc ctgtgtgatc
    46141 accataataa cgaccattaa aggtagtaaa tggacttgca ccagcataac gaccaaagtt
    46201 actggcgata gagttattcg caaccaggaa ggagaattcg ttgaagccac ccttatcaaa
    46261 tttctgcgtt aaagatgtac caaacatcca ggtatctttc cagtcataat acccgttatt
    46321 atcctgatta tctttttccg atgcgctttc atttgctgag acataacgac cgctcaccat
    46381 taaggtggct ttatcccata acgggatatc tttatagcgt aaatcaatgg tattggtatt
    46441 aatctgctgt ttgttttgca ggctgcgatc gtaatcatcg acatcttcac gaaccagcgc
    46501 gatatcaatt ttacccggac cgactttcca gttttccaga cctacacctg cggcagcatc
    46561 ggtacgctgc gttttccagt caagcatctg gatttcaatt ttcggcgcac cgtgtttacc
    46621 cacccagaaa tcagcctctg gcgcaaaggg caggaaacct ttggtggtaa cgtacatatc
    46681 agagaactgc ataaagttct cgccaccggc gttatcgcca aaccagccag tagagtactg
    46741 ctgacccacg ttaccatcca tcatcacgat ggcatcaacc cgtttgccgt tttcgttgta
    46801 gacacgttgt tttaactgca aatcaaacca gccggagtat tcgttaccaa agcggcccag
    46861 agaaccaatt gcccatgatt taggtgaacc atgggaggct gtgccccaac cagaacggta
    46921 gtagccgttg taactaaagc cgatttcatc tttcacaaat ttactgaaat ctttcagagt
    46981 catggaagaa taaatcgagc ctgtctcact ggcatttttc tcttcatttt taaccagtac
    47041 agcatcaggc tttgcggcac gactggtcgg gaatggattg gcggcatacc cttgatcatt
    47101 cgcacttacg ctacgattca ccgtcgctgg cgtatacttt ttcttctctt catctttata
    47161 ttttttcagt tcgctttgcg tttctcttaa cgccttttct aatagctcaa ggcgttgttc
    47221 cacggttaat gattcagcca atgattgtgc ggaaaaggct aacggtgcca ttaataaaat
    47281 ggcagaggta ataatatttc gtctaaacat aatatccctt tatggtgcaa agaaagaatt
    47341 aacgcatcgc atccagactg ttctgaatgc gacgatattt aaggtgcttt attggttatt
    47401 tttttaatga tagcccccga gttttgatca cttctgcata ccatccgaag cttttcttgc
    47461 gcgttcttgt gaggcttcct tcaccattat catcacgatc cacataaata aagccgtagc
    47521 gcttagacat ttgtgaatga gatgcactga ctaaatcaat tggcccccaa ctggtgtacc
    47581 ccataatatc cacaccatcg gcaatcgctt catttacctg taccaggtga tcatttaaat
    47641 aggcaattcg ataatcgtcc tgtatcgaac catccacttc aacgctgtct tttgcgccta
    47701 atccgttctc gacaataaat aacggttttt gataacgatc ccaaagcgta tttaacagaa
    47761 tccgtaatcc aaccggatca atttgccacc cccactctga acttttcaga tgcggatttg
    47821 ggatcatatt cagtatgttg ccctgcgcat ttttattaat gctttcgtcg tgggaaacac
    47881 aaccggtcat gtaataacta aaagagatga aatctacggt atgttttaaa tcttctgcgt
    47941 cactttcagt catctcaatg gtgatattgt ggtcgcggaa gaaacgctgc atatagccgg
    48001 gatactgacc acgcgcctga acatcaccaa agaacatcca gcgccggttc tcttccatgg
    48061 cctgcaacat atcctgtggc tggcaggtga gggggtaaac cagcccaccg agaagcatat
    48121 tgccaatttt cgcttcgggg atcaggctat gacaggcttt aactgcccgc gcactggcaa
    48181 ccagttgatg atggatcgcc tgataaactt ccgcctcgcc actctcttct gccagcccca
    48241 cgcccgtgaa tggcgcgtgt aatgacatgt tgatttcatt aaatgtcagc cataacacaa
    48301 ctttatgttg gtagcgggta aagaccgtgc gtgcgtaatg ctcgaagtga tcgatgaccg
    48361 cccgattagc ccaaccgccg tagtttttca ccagcccata tggcatttcg taatgggata
    48421 acgttaccag cggcttgatc cccgcctgcg ccatttcatc aaacagccgc tcgtaaaacg
    48481 ctaaccccgc ttcattcggt tcggcttcgt cgccctgagg gaaaattcgc gcccaggcaa
    48541 tggaaatacg cagacaggtg aagcccatct cggcaaataa cgcgatatct tccgggtaac
    48601 ggtgataaaa atcgatggcg acatctttga tattctcttt ccccaggatg cgcggttcca
    48661 tttttcccat tacgccatga ggctgtaaat ctgaggtcgt gatccctttg ccatcttcct
    48721 gccatgcgcc ttccacctga ttggcagctg ttgcaccacc ccaaagaaat gtttctggaa
    48781 atgctttcat aattaactcc ttttatcgtt agcgaatgat ggataacagc ggctcaccag
    48841 cgtttatctg caccgtgccg tggggtaata cgtccgtaaa atcatcgcta ttactgatta
    48901 ataccggcgt cgtcagatca aatccggcct cgcgaatagc agggatatca aaagaaatca
    48961 gccgatcgcc tgtattgacc ttgtcaccca cgttgacgtg agcggaaaag aatttgccgt
    49021 ccagttttac ggtgtcgata ccgacatgaa tcaggatctc cacgccatca tctgactcaa
    49081 tgccaatggc gtgtaatgtg gcgaacaacg aagcaattcg acccgccacc ggagaacgca
    49141 cttcaccaac agagggcaga atggcaatac ctttacccaa caggccactg gcaaacgtgg
    49201 tatcagcgac gtgaatgagc ggcacaatat ctcccgtcat cggtgaacag ataccgccct
    49261 gctcaggtgt aataatctct ggtgttttct cttgctgggc aacttgcgct ggctgacgtt
    49321 tagcggtgat gaaatgaagc atcaccgtac cgacaaatgc gcaaccgatg gcaatgacac
    49381 caccaataac gctggcccat acggtgaaat caatccccgt tgacgggatg gtttgcatga
    49441 aggtgaaaat acctggcaaa ccaaaggagt aaactttcgt ttgcgcgtag ccaatgatgg
    49501 tggcccccaa tgccccactt atgcaggcga taacgaaggg gtacttacgc ggcaggttga
    49561 cgccatatac tgccggttcg gtgataccaa acagccccgt caacgccgcc gatcccgcta
    49621 ccactttttt ctgcgcatcg cgttcgcaga ggaagacgcc gagcgccgcc ccgacttgcg
    49681 ccataatggc gggcattaac agcgggatca tggtgtcgta gcccagcacg gtgaagttat
    49741 tgataattaa aggtatcaaa ccccaatgca gaccaaacat cacaaagatt tgccagaagc
    49801 cgcccattac cgcgcctgca aatgcaggaa ccgcctgata aagccagaga taaccggcag
    49861 caatcagttc acttatccag gttgatagcg gccccaccag cagaaatgtg atgggcgtga
    49921 tgaccatcag acatagcaag ggtgtgaaga aatttttgat tgccgaaggt aaccacgcat
    49981 taagtcggcg ttccagaatg ctgcacaacc aggcagaaaa aataatggga ataaccgatg
    50041 acgagtaatt caacaatgtg accggaatac ccaggaaatc caggcccagc gcatccactt
    50101 tttgcccgtt ctcgaaggca gtcagaatta atggatgcac taacgctccg ccaatcacca
    50161 tggcagtaaa tggattgccg ctgaagcgtt tccctgcggt gtagcccagg attatcggga
    50221 agaaccaaaa caaggcatca ctggcgctaa ataaaattaa ataagtacca ctttgttcgg
    50281 tcgtccactg aaaagtgagt gccagagcca acataccttt caggatcccg gttgccgcca
    50341 tcaaaccgat cagaggcgta aaaatacctg aaataacata aacaaagcgg tttaacagat
    50401 tacctttatc atcattttcc ggtgcctgtt gcgctttttc gccaaggcct gccacgctgt
    50461 taaccgccag gaagacatcg gccacatggt tacctatgac aacctgaaac tggccaccgc
    50521 tttccaccac cataataata ccgggggtct ttttcagtac ctcagcttgc gctttgcttt
    50581 catcctttaa cttaaaacgt aatcgcgttg cacaatgcat cagactcaca atgttatctg
    50641 cgcccccgac tcctgcgact attgttctgg ctaactccgt cataacttgc cctctaccgc
    50701 tttgcggcaa aactcaaaaa aaaacctgaa aaaaacggcc tgacgtgaat caagcaattt
    50761 ttttcaggtt ttgcccgctt agtgcggtaa caatccttta ttcagtaata atatttcagt
    50821 gttctttgcg cacgcgctct atatttatgg ctaaaaacat aatctctgca ggtgagattt
    50881 tacgttgata ctgcaaacca ataaaaatgg cgatccgctc cgcacattgc catgcttgcg
    50941 ggtaattttg ctttactgct tgttgtaatg attcatcact atcgttaatc gatgcatgtt
    51001 caataatacg ccaggataaa aatttcagat gcgtaaccag tcgctgataa ctcaagcttt
    51061 cttcctggta attaaggctg aactgaaatt ttattaattg cagcatttct cgcattaatt
    51121 gcgtgacacc tgcaacatcc tccatatttc cgctcatttg ggcactgacc agatgcatgg
    51181 caataaagcc cacttcatct ttcggtaact gcacgcccaa ccgtttatca ataatggtta
    51241 acgcttcttc ccctagctgg aactctttcg ggtaaagtcg ctggatatcc cacagcagcg
    51301 ggttgggcag tagcacgttt tgctgaaagc gtttaatcgc aaactggcaa tggtcagtta
    51361 gcgagatata aatactgtcc tgcaactttc ccagacgctc ctgcgccaga gagataatac
    51421 gatcacaggt tgccatcacc tcaagaggca tatgacttaa gagttcgctt aatcgcccgt
    51481 tcagttcatg actgctcaag gcatactctt tttctattcc acttgagtta attctttcgc
    51541 ctgggcgttt ttgaaagcca attccgcgcc ccatgacgac tttttcccgc tgttgatcat
    51601 caataaccac cacaacatta ttgttgagaa ttttggtgat ttgcatgttc atagcaagga
    51661 ccttttttat aaacaaaaaa acccgacttc accagtattc tctggttatg tcaggttttg
    51721 cctgcgaatg cagtaacaat ccagtcatta attaatggtt tttataacga acatccaggt
    51781 tcgaaaatta atttagttgc gtgcagaaaa aatagcaatg ctctatttat aaaagtatga
    51841 ccatgctcgc ag
//
LOCUS       DAHHTG010000015        51580 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.015, whole genome shotgun sequence.
ACCESSION   DAHHTG010000015 DAHHTG010000000
VERSION     DAHHTG010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 51580)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 51580)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..51580
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.015"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            complement(90..737)
                     /gene="ychE"
                     /locus_tag="NBI74_000898"
     CDS             complement(90..737)
                     /gene="ychE"
                     /locus_tag="NBI74_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YchE"
                     /protein_id="HCD1931271.1"
                     /translation="MIQTFFDFPVYFKFFIGLFALVNPVGIIPVFISMTSYQTAAARN
                     KTNLTANLSVAIILWISLFLGDTILQLFGISIDSFRIAGGILVVTIAMSMISGKLGED
                     KQNKQEKSETAVRESIGVVPLALPLMAGPGAISSTIVWGTRYHSISYLFGFFVAIALF
                     ALCCWGLFRMAPWLVRVLRQTGINVITRIMGLLLMALGIEFIVTGIKGIFPGLLN"
     gene            1214..3889
                     /gene="adhE"
                     /locus_tag="NBI74_000899"
                     /gene_synonym="adhC"
     CDS             1214..3889
                     /gene="adhE"
                     /locus_tag="NBI74_000899"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HCD1931272.1"
                     /translation="MAVTNVAELNALVERVKKAQREYASFTQEQVDKIFRAAALAAAD
                     ARIPLAKMAVAESGMGIVEDKVIKNHFASEYIYNAYKDEKTCGVLSEDDTFGTITIAE
                     PIGIICGIVPTTNPTSTAIFKSLISLKTRNAIIFSPHPRAKDATNKAADIVLQAAIAA
                     GAPKDLIGWIDQPSVELSNALMHHPDINLILATGGPGMVKAAYSSGKPAIGVGAGNTP
                     VVIDETADIKRAVASVLMSKTFDNGVICASEQSVVVVDSVYDAVRERFATHGGYLLQG
                     KELKAVQDVILKNGALNAAIVGQPAYKIAELAGFSVPENTKILIGEVTVVDESEPFAH
                     EKLSPTLAMYRAKDFEDAVEKAEKLVAMGGIGHTSCLYTDQDNQPARVSYFGQKMKTA
                     RILINTPASQGGIGDLYNFKLAPSLTLGCGSWGGNSISENVGPKHLINKKTVAKRAEN
                     MLWHKLPKSIYFRRGSLPIALDEVITDGHKRALIVTDRFLFNNGYADQITSVLKAAGV
                     ETEVFFEVEADPTLSIVRKGAELANSFKPDVIIALGGGSPMDAAKIMWVMYEHPETHF
                     EELALRFMDIRKRIYKFPKMGVKAKMIAVTTTSGTGSEVTPFAVVTDDTTGQKYPLAD
                     YALTPDMAIVDANLVMDMPKSLCAFGGLDAVTHAMEAYVSVLASEFSDGQALQALKLL
                     KEYLPASYHEGSKNPVARERVHSAATIAGIAFANAFLGVCHSMAHKLGSQFHIPHGLA
                     NALLICNVIRYNANDNPTKQTAFSQYDRPQARRRYAEIADHLGLSAPGDRTAAKIEKL
                     LAWLETLKAELGIPKSIREAGVQEADFLANVDKLSEDAFDDQCTGANPRYPLISELKQ
                     ILLDTYYGRDYVEGETAAKKEAAPAKAEKKAKKSA"
     gene            4014..5061
                     /locus_tag="NBI74_000900"
                     /pseudo
     CDS             4014..5061
                     /locus_tag="NBI74_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418698.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
     gene            complement(5347..5964)
                     /gene="tdk"
                     /locus_tag="NBI74_000901"
     CDS             complement(5347..5964)
                     /gene="tdk"
                     /locus_tag="NBI74_000901"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HCD1931273.1"
                     /translation="MAQLYFYYSAMNAGKSTALLQSSYNYQERGMRTVVYTAEIDDRF
                     GAGKVSSRIGLSSPAKLFNQNSSLFDEIRAEHEQQAIHCVLVDECQFLTRQQVYELSE
                     VVDQLDIPVLCYGLRTDFRGELFIGSQYLLAWSDKLVELKTICFCGRKASMVLRLDQA
                     GRPYNEGEQVVIGGNERYVSVCRKHYKEALEVGSLTAIQERHRHD"
     gene            6568..6981
                     /gene="hns"
                     /locus_tag="NBI74_000902"
     CDS             6568..6981
                     /gene="hns"
                     /locus_tag="NBI74_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator H-NS"
                     /protein_id="HCD1931274.1"
                     /translation="MSEALKILNNIRTLRAQARECTLETLEEMLEKLEVVVNERREEE
                     SAAAAEVEERTRKLQQYREMLIADGIDPNELLNSLAAVKSGTKAKRAQRPAKYSYVDE
                     NGETKTWTGQGRTPAVIKKAMDEQGKSLDDFLIKQ"
     gene            complement(7126..8034)
                     /gene="galU"
                     /locus_tag="NBI74_000903"
     CDS             complement(7126..8034)
                     /gene="galU"
                     /locus_tag="NBI74_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HCD1931275.1"
                     /translation="MAAINTKVKKAVIPVAGLGTRMLPATKAIPKEMLPLVDKPLIQY
                     VVNECIAAGITEIVLVTHSSKNSIENHFDTSFELEAMLEKRVKRQLLDEVQSICPPHV
                     TIMQVRQGLAKGLGHAVLCAHPVVGDEPVAVILPDVILDEYESDLSQDNLAEMIRRFD
                     ETGHSQIMVEPVADVTAYGVVDCKGVELAPGESVPMVGVVEKPKADVAPSNLAIVGRY
                     VLSADIWPLLAKTPPGAGDEIQLTDAIDMLIEKETVEAYHMKGKSHDCGNKLGYMQAF
                     VEYGIRHNTLGTEFKAWLEEEMGIKK"
     gene            complement(8236..9249)
                     /gene="rssB"
                     /locus_tag="NBI74_000904"
     CDS             complement(8236..9249)
                     /gene="rssB"
                     /locus_tag="NBI74_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RssB"
                     /protein_id="HCD1931276.1"
                     /translation="MTQPLVGKQILIVEDEQVFRSLLDSWFSSLGATTVLAADGVDAL
                     ELLGGFTPDLMICDIAMPRMNGLKLLEHIRNRGDQTPVLVISATENMADIAKALRLGV
                     EDVLLKPVKDLNRLREMVFACLYPSMFNSRVEEEERLFRDWDAMVDNPAAAAKLLQEL
                     QPPVQQVISQCRVNYRQLVAADKPGLVLDIAALSENDLAFYCLDVTRAGHNGVLAALL
                     LRALFNGLLQEQLAHQNQRLPELGALLKQVNHLLRQANLPGQFPLLVGYYHRELKNLI
                     LVSAGLNATLNTGEHQVQISNGVPLGTLGNAYLNQLSQRCDAWQCQIWGTGGRLRLML
                     SAE"
     gene            complement(9341..10246)
                     /gene="rssA"
                     /locus_tag="NBI74_000905"
     CDS             complement(9341..10246)
                     /gene="rssA"
                     /locus_tag="NBI74_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase RssA"
                     /protein_id="HCD1931277.1"
                     /translation="MRKIKIGLALGSGAARGWSHIGVINALKKVGIEIDIVAGCSIGS
                     LVGAAYACDRLSALEDWVTSFSYWDVLRLMDLSWQRGGLLRGERVFNQYREIMPETEI
                     ENCSRRFAAVATNLSTGRELWFTEGDLHLAIRASCSIPGLMAPVAHNGYWLVDGAVVN
                     PIPISLTRALGADIVIAVDLQHDAHLMQQDLLSFNVSEENSENGDSLPWHARLKERLG
                     SITTRRAVTAPTATEIMTTSIQVLENRLKRNRMAGDPPDILIQPVCPQISTLDFHRAH
                     AAIAAGQLAVEKKMDELLPLVRTNI"
     gene            10359..10817
                     /locus_tag="NBI74_000906"
     CDS             10359..10817
                     /locus_tag="NBI74_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YchJ family protein"
                     /protein_id="HCD1931278.1"
                     /translation="MSQLCPCGSAVEYSLCCHPYVSGEKVAPDPEHLMRSRYCAFVMQ
                     DADYLIKTWHPSCGAAALRAELIAGFAHTEWLGLTVFEHCWQDGGDIGFVSFVARFTE
                     GGKTGAIIERSRFLKENGQWYYIDGTRPQFGRNDPCPCGSGKKFKKCCGQ"
     gene            10867..11709
                     /gene="purU"
                     /locus_tag="NBI74_000907"
     CDS             10867..11709
                     /gene="purU"
                     /locus_tag="NBI74_000907"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HCD1931279.1"
                     /translation="MHSLQRKVLRTICPDQKGLIARITNICYKHELNIVQNNEFVDHR
                     TGRFFMRTELEGIFNDSTLLADLDSALPEGSVRELNPAGRRRIVILVTKEAHCLGDLL
                     MKANYGGLDVEIAAVIGNHDNLRSLVERFDIPFELVSHEGLSRNEHDQKMADAIDAYQ
                     PDYVVLAKYMRVLTPEFVARFPNKIINIHHSFLPAFIGARPYHQAYERGVKIIGATAH
                     YVNDNLDEGPIIMQDVIHVDHTYTAEDMMRAGRDVEKNVLSRALYKVLAQRVFVYGNR
                     TIIL"
     gene            11869..11953
                     /locus_tag="NBI74_000908"
     tRNA            11869..11953
                     /locus_tag="NBI74_000908"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11903..11905,aa:Tyr,seq:gta)
     gene            11987..12071
                     /locus_tag="NBI74_000909"
     tRNA            11987..12071
                     /locus_tag="NBI74_000909"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12021..12023,aa:Tyr,seq:gta)
     gene            complement(12088..13179)
                     /locus_tag="NBI74_000910"
     CDS             complement(12088..13179)
                     /locus_tag="NBI74_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001526934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA desaturase"
                     /protein_id="HCD1931280.1"
                     /translation="MNLFNPDRLITFPADDPQLVKQLQSGTKVYLAKSGDHRYADGWA
                     FVKMAVLIIACFSCYLLALFQRQWGLYLLWYLAMMFCAMLLAVNVVHDASHDAFLRGK
                     KTNARLNRWVAFPMGLDPDCWRVRHVRFHHGFTNIEFYDPDTAENGILRQTPWQRWRP
                     FMRQQHRYWPLVAALTFPWYIWVVDWLDRAGFTPVTRHLALRGFAGWGYFLAGKLAHC
                     ALCLIIPWLLSDFSFMTILLTYLLSQLLASLIFVTLIIGTHWAKGHTQLPPEEGKMPV
                     GRLAHTFATTFDWTPQPAWLGYWLGGINLHLTHHLFPHWHHRHYSALSRIIAQIASQQ
                     GLDYQLLTLADLLRLQQQFLRRMGEKPKV"
     gene            complement(13166..14290)
                     /locus_tag="NBI74_000911"
     CDS             complement(13166..14290)
                     /locus_tag="NBI74_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001281624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HCD1931281.1"
                     /translation="MSDLLFPIISMVLLVLAEAGVLQFTGRQKVDWLDVIFNINSGHM
                     MLWLFRCLEVLCYGLVVNHFSLGLFDNVPVILLWLFTILAWDFGFYWLHRWHHEMRPL
                     WAVHVVHHQGEHYNLSLGVRNSWYSSLTSIPFFMLLAMLGVPLQVFLAVSIIHYSWQF
                     FNHNALTPKLGWLEKVLITPSHHRVHHLNQKQYADTNYGGTFLLWDKLFGSYSAGPEY
                     DVGYGVKNSRLSCNPMRESNLPFLRLMGFCSFGKAIEGRYQSSPWAVGAGVILLFMLV
                     LGYIQQYGYGIEFITSEQAVLFVLLAAGSVALGGMSDGQHWGMILWCSVTLSLLYFIV
                     YMCSWSTLLWKGLVGILLAHCLLFVIGVGRKSLRKQYEPV"
     gene            complement(14283..14885)
                     /locus_tag="NBI74_000912"
     CDS             complement(14283..14885)
                     /locus_tag="NBI74_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002432219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol phosphorylceramide synthase"
                     /protein_id="HCD1931282.1"
                     /translation="MSNSLLRLKQMLLGWGTVGVIYNFSDYLQGEGYQLTPSIIDNLI
                     AFSPSAVWLYLSFFFIVPLGYLSTPLCHLRWLARSMQISALVAGVFYLLWPTTMHYPS
                     FDQMGISAGALNRLIAIDSSQNCFPSLHAALTLLAVWAIAKKGNRWLTLMSVVWAIAI
                     AFSILQLRRHLFIDLVGGALLASICGWICATCEKRKTQYE"
     gene            complement(14882..16168)
                     /locus_tag="NBI74_000913"
     CDS             complement(14882..16168)
                     /locus_tag="NBI74_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000612538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoF synthetase"
                     /protein_id="HCD1931283.1"
                     /translation="MIPLMLIWRYFRTRRLHFTDREALENWQAKRLHQFRQNVLSHSP
                     WFQRYLTLPFNQWPMMDKALMMTHFDEMNTAGLKRDELLDCAMRSEQSRDFKPCVGKF
                     SVGLSSGTSGRRGLFVVSPHEQQMWAAGVLAKVLPDDLFAKERVALFLRADNNLYQSV
                     NNRWLSLDFYDLLAPFQAQLKLLQQRAPTIIVAPAQVLRALALEVMAGELTLKVKKVI
                     SVAEVLEPQDRELLRNVFHNVGEIYQATEGFLASTCRCGTLHLNEEFVHIEPQWLDEH
                     RFVPIVTDFTRTTQPIVRYRLDDVLVASEQPCPCGSATMAIARIEGRQDDQLQLPTRS
                     GDIQTVFADACSRVLAMTLPLTVDYRLLQTGSAQLTLIADCELWMLEHCREALNALFI
                     RQDIAVGQLIWSLELQTVPVSFTAKRRRIVRQWGRE"
     gene            complement(16165..16971)
                     /locus_tag="NBI74_000914"
     CDS             complement(16165..16971)
                     /locus_tag="NBI74_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HCD1931284.1"
                     /translation="MAKITAFETGWCTHISCIALKGAGLKVCKFPARAWLLEVGDKRW
                     LWDTGYSSWFEHYTQSGVFRLYRQVTPVYFDPQQALVRQLSATGLAAKDVDAIILSHF
                     HADHIAGLRDFPDVSCICSGEGWRQVRTLRGVTALRQAFVPGLIPENFDSSLRFIESF
                     ASVNLPAQLAPFECGYLLPESNGEIILVPLPGHAAGHIGAFVLTDNGWTLLAGDAAWA
                     PANYRELRGPSPLAHLVMSDSHAYYHTLGQLHQLWQNGVVEIRLCHEGDL"
     gene            complement(16949..17965)
                     /locus_tag="NBI74_000915"
     CDS             complement(16949..17965)
                     /locus_tag="NBI74_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001311639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HCD1931285.1"
                     /translation="MKILVTGATSGLGRNAVQYLLQSGESVVATGRNCEVGRSLTNAG
                     AQFVALDLTEATEDDCARLMDGCDAVWHCAAKSSPWGDKTAFWLANVKVTHTLAQAAG
                     RLNIPRFIHISTPAVYFDFQHHYDLPETYLARAFSSHYASSKYAAEQVIAGAVSRYSA
                     TRFILLRPRGLFGPHDQVIVPRLMQQLQQGGGCLRLPRGGDALLDLTFVQNVVYAMRL
                     ATNVEGLRSGSIYNISNHQPQQLSVMLDALLHQQLGLSYRIAAVPWSLLSLIARGLEL
                     YGKCVNQEPVITRYSAGTLCFDMTLSAQKAITELGYRPRFSMEQGIAITGQWLREHGK
                     NNRI"
     gene            complement(17962..18969)
                     /locus_tag="NBI74_000916"
     CDS             complement(17962..18969)
                     /locus_tag="NBI74_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001385314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HCD1931286.1"
                     /translation="MARPNQPTISAALKIIATGIALPPDQITSKQLDVQLNKPVGYVE
                     RRSGICWRYFARNDASQAELAAEALHNALVAAALNPSDIDLLICASAIAVQALPCSAI
                     HILKAAAMPVGVAGFDINSSCVSFISALEVAAGLLNCGAYRRIAIVSADIASRGIDWD
                     HEESSLIFGDGAACAIVERGDGRSGIIASLIEMYPEGSELCEIRAGGTRRNPRSGVSD
                     SDFLFHMQGKPLFRFASSLIEDYFSRLLQKSSLQLSDIGTVVPHQASHLSLEHMRKRL
                     RVPESALIDVYRFYGNQVAASIPTALHEAVISGRFTADKPVMLIGTAAGLTLAGMVLL
                     P"
     gene            complement(19113..19790)
                     /gene="narI"
                     /locus_tag="NBI74_000917"
     CDS             complement(19113..19790)
                     /gene="narI"
                     /locus_tag="NBI74_000917"
                     /EC_number="1.7.99.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HCD1931287.1"
                     /translation="MQFLNMFFFDIYPYIAGAVFLIGSWLRYDYGQYTWRAASSQMLD
                     RKGMNLASNLFHIGILGIFVGHFFGMLTPHWMYEAWLPIEVKQKMAMFAGGASGVLCL
                     IGGVLLLKRRLFSPRVRATTTGADILILSLLVIQCALGLLTIPFSAQHMDGSEMMKLV
                     GWAQSVVTFHGGASQHLDGVAFIFRLHLVLGMTLFLLFPFSRLVHIWSVPVEYLTRKY
                     QLVRARH"
     gene            complement(19790..20500)
                     /gene="narJ"
                     /locus_tag="NBI74_000918"
     CDS             complement(19790..20500)
                     /gene="narJ"
                     /locus_tag="NBI74_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HCD1931288.1"
                     /translation="MIELVIVSRLLEYPDAALWQHQQEMFEAIAASKNLPKEDAHALG
                     IFLRDLTTMDPLDAQAQYSELFDRGRATSLLLFEHVHGESRDRGQAMVDLLAQYEQHG
                     LQLNSRELPDHLPLYLEYLAQLPQSEAVEGLKDIAPILALLSARLQQRESRYAVLFDL
                     LLKLANTAIDSDKVAEKIADEARDDTPQALDAVWEEEQVKFFADKGCGDSAITAHQRR
                     FAGAVAPQYLNITTGGQH"
     gene            complement(20497..22035)
                     /gene="narH"
                     /locus_tag="NBI74_000919"
     CDS             complement(20497..22035)
                     /gene="narH"
                     /locus_tag="NBI74_000919"
                     /EC_number="1.7.99.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HCD1931289.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTSREGVEYAWFNNV
                     ETKPGQGFPTDWENQEKYKGGWIRKINGKLQPRMGNRAMLLGKIFANPHLPGIDDYYE
                     PFDFDYQNLHTAPEGSKSQPIARPRSLITGERMAKIEKGPNWEDDLGGEFDKLAKDKN
                     FDNIQKAMYSQFENTFMMYLPRLCEHCLNPACVATCPSGAIYKREEDGIVLIDQDKCR
                     GWRMCITGCPYKKIYFNWKSGKSEKCIFCYPRIEAGQPTICSETCVGRIRYLGVLLYD
                     ADAIERAASTENEKDLYQRQLDVFLDPNDPKVIEQAIKDGIPLSVIEAAQQSPVYKMA
                     MEWKLALPLHPEYRTLPMVWYVPPLSPIQSAADAGELGSNGILPDVESLRIPVQYLAN
                     LLTAGDTKPVLRALKRMLAMRHYKRAETVDGKVDTRALEEVGLTEAQAQEMYRYLAIA
                     NYEDRFVVPSSHRELAREAFPEKNGCGFTFGDGCHGSDTKFNLFNSRRIDAIDVTSKT
                     EPHP"
     gene            complement(22032..25775)
                     /locus_tag="NBI74_000920"
     CDS             complement(22032..25775)
                     /locus_tag="NBI74_000920"
                     /EC_number="1.7.99.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit alpha"
                     /protein_id="HCD1931290.1"
                     /translation="MSKFLDRFRYFKQKGETFADGHGQLLNTNRDWEDGYRQRWQHDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWETQQTDYPRTRPDLPNHEPRGCPRGASYSWY
                     LYSANRLKYPMMRKRLMKMWREAKALHSDPVEAWASIIEDADKAKSFKQARGRGGFVR
                     SSWQEVNELIAASNVYTIKNYGPDRVAGFSPIPAMSMVSYASGARYLSLIGGTCLSFY
                     DWYCDLPPASPQTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTVAVTPDYAEIAKLCDLWLAPKQGTDAAMALAMGHVMLREFHLDNPSQYFTDYVRRY
                     TDMPMLVMLEERDGYYAAGRMLRAADLVDALGQENNPEWKTVAFNTNGEMVAPNGSIG
                     FRWGEKGKWNLEQRDGKTGEETELQLSLLGSQDEIAEVGFPYFGGDGTEHFNKVELEN
                     VLLHKLPVKRLQLADGSTALVTTVYDLTLANYGLERGLNDVNCATSYDDVKAYTPAWA
                     EQITGVSRSQIIRIAREFADNADKTHGRSMIIVGAGLNHWYHLDMNYRGLINMLIFCG
                     CVGQSGGGWAHYVGQEKLRPQTGWQPLAFALDWQRPARHMNSTSYFYNHSSQWRYETV
                     TAEELLSPMADKSRYTGHLIDFNVRAERMGWLPSAPQLGTNPLTIAREAEKAGMNPVD
                     YTVKSLKEGSIRFAAEQPENGKNHPRNLFIWRSNLLGSSGKGHEFMLKYLLGTEHGIQ
                     GKDLGQQGGVKPEEVDWQDNGLEGKLDLVVTLDFRLSSTCLYSDIILPTATWYEKDDM
                     NTSDMHPFIHPLSAAVDPAWEAKSDWEIYKAIAKKFSEVCVGHLGKETDIVTLPIQHD
                     SAAELAQPLDVKDWKKGECDLIPGKTAPHIMVVERDYPATYERFTSIGPLMEKIGNGG
                     KGIAWNTQSEMDLLRKLNYTKAEGPAKGQPMLNTAIDAAEMILTLAPETNGQVAVKAW
                     AALSEFTGRDHTHLALNKEDEKIRFRDIQAQPRKIISSPTWSGLEDEHVSYNAGYTNV
                     HELIPWRTLSGRQQLYQDHQWMRDFGESLLVYRPPIDTRSVKEVMGQKSNGNPEKALN
                     FLTPHQKWGIHSTYSDNLLMLTLGRGGPVVWLSEADAKELGIADNDWIEVFNSNGALT
                     ARAVVSQRVPAGMTMMYHAQERIVNLPGSEITQQRGGIHNSVTRITPKPTHMIGGYAH
                     LAYGFNYYGTVGSNRDEFVVVRKMKNIDWLDGEGNDQVQESVK"
     gene            complement(26168..27559)
                     /gene="narK"
                     /locus_tag="NBI74_000921"
     CDS             complement(26168..27559)
                     /gene="narK"
                     /locus_tag="NBI74_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate transporter NarK"
                     /protein_id="HCD1931291.1"
                     /translation="MSHSSAPERATGAVITDWRPEDPAFWQQRGQRIASRNLWISVPC
                     LLLAFCVWMLFSAVAVNLPKVGFNFTTDQLFMLTALPSVSGALLRVPYSFMVPIFGGR
                     RWTAFSTGILIIPCVWLGFAVQDTSTPYSVFIIISLLCGFAGANFASSMANISFFFPK
                     QKQGGALGLNGGLGNMGVSVMQLVAPLVVSLSIFAVFGSQGVKQPDGTELYLANASWI
                     WVPFLAIFTIAAWFGMNDLATSKASIKEQLPVLKRGHLWIMSLLYLATFGSFIGFSAG
                     FAMLSKTQFPDVQILQYAFFGPFIGALARSAGGALSDRLGGTRVTLVNFILMAIFSGL
                     LFLTLPTDGQGGSFMAFFAVFLALFLTAGLGSGSTFQMISVIFRKLTMDRVKAEGGSD
                     ERAMREAATDTAAALGFISAIGAIGGFFIPKAFGSSLALTGSPVGAMKVFLIFYIACV
                     VITWAVYGRHSKK"
     gene            27898..29694
                     /gene="narX"
                     /locus_tag="NBI74_000922"
     CDS             27898..29694
                     /gene="narX"
                     /locus_tag="NBI74_000922"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarX"
                     /protein_id="HCD1931292.1"
                     /translation="MLKRCLSPLTLVNQVALIVLLSTAIGLAGMAVSGWLVQGVQGSA
                     HAINKAGSLRMQSYRLLAAVPLSEKDKPLIKEMEQTAFSAELTRAAERDGQLAQLQGL
                     QDYWRNELIPALIRAQNRETVSADVSQFVAGLDQLVSGFDRTTEMRIETVVLVHRVMA
                     VFMALLLVFTIIWLRARLLQPWRQLLAMASAVSHRDFTQRANISGRNEMAMLGTALNN
                     MSAELAESYAVLEQRVQEKTAGLEHKNQILSFLWQANRRLHSRAPLCERLSPVLNGLQ
                     NLTLLRDIELRVYDTDDEENHQEFTCQSDMTCDDKGCQLCPRGVLPVGDRGTTLKWRL
                     ADTHTQYGILLATLPQGRHLSHDQQQLVDTLVEQLTATLALDRHQERQQQLIVMEERA
                     TIARELHDSIAQSLSCMKMQVSCLQMQGDALPESSRELLSQIRNELNASWVQLRELLT
                     TFRLQLTEPGLRPALEASCEEYSAKFGFPVKLDYQLPPRLVPSHQAIHLLQIAREALS
                     NALKHSQASEVVVTVAQNDNQVKLTVQDNGCGVPENAIRSNHYGMIIMRDRAQSLRGD
                     CRVRRRESGGTEVVVTFIPEKTFTDVQGDTHE"
     gene            29687..30337
                     /gene="narL"
                     /locus_tag="NBI74_000923"
     CDS             29687..30337
                     /gene="narL"
                     /locus_tag="NBI74_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator NarL"
                     /protein_id="HCD1931293.1"
                     /translation="MSNQEPATILLIDDHPMLRTGVKQLISMAPDITVVGEASNGEQG
                     IELAESLDPDLILLDLNMPGMNGLETLDKLREKSLSGRIVVFSVSNHEEDVVTALKRG
                     ADGYLLKDMEPEDLLKALHQAAAGEMVLSEALTPVLAASLRANRATTERDVNQLTPRE
                     RDILKLIAQGLPNKMIARRLDITESTVKVHVKHMLKKMKLKSRVEAAVWVHQERIF"
     gene            complement(30338..31732)
                     /gene="ychO"
                     /locus_tag="NBI74_000924"
     CDS             complement(30338..31732)
                     /gene="ychO"
                     /locus_tag="NBI74_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter invasin YchO"
                     /protein_id="HCD1931294.1"
                     /translation="MSRFVPRIIPFYLLLLAAGGTANAQSTFEQKAANHFENSNDGLP
                     DLGMAPEHHDGEKHFAEIVKDFGETSMNDNGLDTGEQAKAFALGKVRDALSQQVNQHV
                     ESWLSPWGNASVDVKVDNEGHFTGSRGSWFVPLQDNDRYLTWSQLGLTQQDDGLVSNV
                     GVGQRWARGSWLVGYNTFYDNLLDENLQRAGFGAEAWGEYLRLSANFYQPFAAWHEQT
                     ATQEQRMARGYDLTARMRMPFYQHLNTSVSVEQYFGDRVDLFNSGTGYHNPVALSLGL
                     NYTPVPLVTVTAQHKQGESGENQNNLGLNLNYRFGVPLKKQLSAGEVVESQSLRGSRY
                     DNPQRNNLPTLEYRQRKTLTVFLATPPWDLKPGETVPLKLQIRSRYGIRQLIWQGDTQ
                     ILSLTPGAQANSEEGWTLIMPDWQNGEGASNHWRLSVVVEDNQGQRVSSNEITLTLVE
                     PFDALSNDELRWEP"
     gene            31917..32270
                     /gene="ychN"
                     /locus_tag="NBI74_000925"
     CDS             31917..32270
                     /gene="ychN"
                     /locus_tag="NBI74_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/F sulfur relay family protein YchN"
                     /protein_id="HCD1931295.1"
                     /translation="MQKIVIVANGAPYGSESLFNSLRLAIALREQENNLDLRLFLMSD
                     AVTAGLRGQKPGEGYNIQQMLEILTAQNVPVKLCKTCTDGRGISTLPLIDGVEIGTLV
                     ELAQWTLSADKVLTF"
     gene            complement(32314..33009)
                     /gene="chaC"
                     /locus_tag="NBI74_000926"
     CDS             complement(32314..33009)
                     /gene="chaC"
                     /locus_tag="NBI74_000926"
                     /EC_number="4.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-specific
                     gamma-glutamylcyclotransferase"
                     /protein_id="HCD1931296.1"
                     /translation="MITRDFLMNADCKTAFGAIEESLLWSAEQRAASLAATLACRPDE
                     GPVWIFGYGSLMWNPALEFTESCTGTLVGWHRAFCLRLTAGRGTAHQPGRMLALKEGG
                     RTTGVAYRLPEETLEQELTLLWKREMITGCYLPTWCQLDLDDGRTVNAIVFIMDPRHP
                     EYESDTRAQVIAPLIAAASGPLGTNAQYLFSLEQELIKLGMQDDGLNDLLVSVKKLLA
                     ENYPDGVLRPGFA"
     gene            complement(33158..33397)
                     /gene="chaB"
                     /locus_tag="NBI74_000927"
     CDS             complement(33158..33397)
                     /gene="chaB"
                     /locus_tag="NBI74_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000120702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative cation transport regulator ChaB"
                     /protein_id="HCD1931297.1"
                     /translation="MSYKTKSDLPESVKHVLPSHAQDIYKEAFNSAWDQYKDKEDRRD
                     DASREETAHKVAWAAVKHEYAKGDDDKWHKKSSNR"
     gene            33667..34767
                     /gene="chaA"
                     /locus_tag="NBI74_000928"
     CDS             33667..34767
                     /gene="chaA"
                     /locus_tag="NBI74_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-potassium/proton antiporter ChaA"
                     /protein_id="HCD1931298.1"
                     /translation="MSNAQEAVKTRHKETSLIFPVLALVVLFLWGSSQTLPVVIAINL
                     LALIGILSSAFSVVRHADVLAHRLGEPYGSLILSLSVVILEVSLISALMATGDAAPTL
                     MRDTLYSIIMIVTGGLVGFSLLLGGRKFATQYMNLFGIKQYLIALFPLAIIVLVFPMA
                     LPAANFSTGQALLVALISAAMYGVFLLIQTKTHQSLFVYEHEDDSDDDDPHHGKPSAH
                     SSLWHAIWLIVHLIAVIAVTKMNASPLETLLDSMNAPVAFTGFLVALLILSPEGLGAL
                     KAVLNNQVQRAMNLFFGSVLATISLTVPVVTLIAFMTGNELQFALGAPEMVVMVASLV
                     LCHISFSTGRTNVLNGAAHLALFAAYLMTIFA"
     gene            35172..35279
                     /locus_tag="NBI74_000929"
     CDS             35172..35279
                     /locus_tag="NBI74_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HCD1931299.1"
                     /translation="MTLAQFAMIFWHNLAAPILAGIITAVIVSWWRNRK"
     gene            complement(35428..36282)
                     /gene="kdsA"
                     /locus_tag="NBI74_000930"
     CDS             complement(35428..36282)
                     /gene="kdsA"
                     /locus_tag="NBI74_000930"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HCD1931300.1"
                     /translation="MKQKVVSIGDINVANDLPFVLFGGMNVLESRDLAMRICEHYVTV
                     TQKLGIPYVFKASFDKANRSSIHSYRGPGLEEGMKIFQELKQTFGVKIITDVHEPSQA
                     QPVADVVDVIQLPAFLARQTDLVEAMAKTGAVINVKKPQFVSPGQMGNIVDKFKEGGN
                     EKVILCDRGANFGYDNLVVDMLGFSIMKKVSGNSPVIFDVTHALQCRDPFGAASGGRR
                     AQVAELARAGMAVGLAGLFIEAHPDPEHAKCDGPSALPLAKLEPFLKQMKAIDDLVKG
                     FEELDTSK"
     gene            complement(36318..37127)
                     /locus_tag="NBI74_000931"
     CDS             complement(36318..37127)
                     /locus_tag="NBI74_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat-containing protein"
                     /protein_id="HCD1931301.1"
                     /translation="MRSLADFEFNKAPLCEGMILACEAIRRDFPSQDVYDELERLVSL
                     AKEEISQLLPLEEQLEKLIALFYGDWGFKASRGVYRLSDALWLDQVLKNRQGSAVSLG
                     AVLLWVANRLDLPLLPVIFPTQLILRIECPDGEIWLINPFNGESLSEHMLDVWLKGNI
                     SPSAELFYEDLDEADNIEVIRKLLDTLKASLMEENQMELALRTSEALLQFNPEDPYEI
                     RDRGLIYAQLDCEHVALNDLSYFVEQCPEDPISEMIRAQINNIAHKHIVLH"
     gene            complement(37131..37523)
                     /locus_tag="NBI74_000932"
     CDS             complement(37131..37523)
                     /locus_tag="NBI74_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SirB family protein"
                     /protein_id="HCD1931302.1"
                     /translation="MTSFSTLLSVHLISIALSVGLLTLRFWLRYQKHPQAFARWTRIV
                     PPVVDTLLLLSGIALMAKAHIQPFSGQAQWLTEKLFGVIIYIVLGFIALDYRRMHSQQ
                     ARIIAFPLALVVLYIIIKLATTKVPLLG"
     gene            complement(37520..38353)
                     /gene="prmC"
                     /locus_tag="NBI74_000933"
     CDS             complement(37520..38353)
                     /gene="prmC"
                     /locus_tag="NBI74_000933"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HCD1931303.1"
                     /translation="MEYQHWLREAISQLQASESPRRDAEILLEHVTGKGRTFILAFGE
                     TQLTDEQCQQLDALLTRRRDGEPIAHLTGVREFWSLPLFVSPATLIPRPDTECLVEQA
                     LARLPEQPCRILDLGTGTGAIALALASERPDCEIIAVDRMPDAVALAQRNAQHLAIKN
                     IHILQSDWFSALAGQQFAMIVSNPPYIDEQDPHLQQGDVRFEPLTALVAADSGMADIV
                     HIIEQSRNALVSGGFLLLEHGWQQGEAVRQAFILAGYHDVETCRDYGDNERVTLGRYY
                     Q"
     gene            complement(38353..39435)
                     /gene="prfA"
                     /locus_tag="NBI74_000934"
     CDS             complement(38353..39435)
                     /gene="prfA"
                     /locus_tag="NBI74_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012132158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HCD1931304.1"
                     /translation="MKPSIVAKLEALHERHEEVQALLGDAQTIADQERFRALSREYAQ
                     LSDVSRCFTDWQQVQEDIETAQMMLDDPEMREMAQDELREAKEKSEQLEQQLQVLLLP
                     KDPDDERNAFLEVRAGTGGDEAALFAGDLFRMYSRYAEARRWRVEIMSASEGEHGGYK
                     EIIAKISGDGVYGRLKFESGGHRVQRVPATESQGRIHTSACTVAVMPELPDAELPDIN
                     PADLRIDTFRSSGAGGQHVNTTDSAIRITHLPTGIVVECQDERSQHKNKAKALSVLGA
                     RIHAAEMAKRQQAEASTRRNLLGSGDRSDRNRTYNFPQGRVTDHRINLTLYRLDEVME
                     GKLDMLIEPIIQEHQADQLAALSEQE"
     gene            complement(39477..40733)
                     /gene="hemA"
                     /locus_tag="NBI74_000935"
     CDS             complement(39477..40733)
                     /gene="hemA"
                     /locus_tag="NBI74_000935"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041689438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HCD1931305.1"
                     /translation="MTLLALGINHKTAPVSLRERVSFSPDKLDQALDSLLAQPMVQGG
                     VVLSTCNRTELYLSVEERDDLQEALIRWLCDYHNLNEDDLRNSLYWHQDNDAVSHLMR
                     VASGLDSLVLGEPQILGQVKKAFADSQKGHMKASELERMFQKSFSVAKRVRTETDIGA
                     SAVSVAFAACTLARQIFESLSTVTVLLVGAGETIELVARHLREHKVQKMIIANRTRER
                     AQILADEVGAEIIALSDIDERLREADIIISSTASPLPIIGKGMVERALKSRRNQPMLL
                     VDIAVPRDVEPEVGKLANAYLYSVDDLQSIISHNLAQRKAAAVEAETIVAQETSEFMA
                     WLRAQSASETIREYRSQAEHVRDELTAKALAALEQGGDAQAIMQDLAWKLTNRLIHAP
                     TKSLQQAARDGDNERLNILRDSLGLE"
     gene            40947..41570
                     /gene="lolB"
                     /locus_tag="NBI74_000936"
     CDS             40947..41570
                     /gene="lolB"
                     /locus_tag="NBI74_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein localization protein LolB"
                     /protein_id="HCD1931306.1"
                     /translation="MTLPDFRFIRLLPLAALVLTACSVTTPKGPGKSPDSPQWRQHQQ
                     DVRNLNQYQTRGAFAYISDQQKVYARFFWQQTGQDRYRLLLTNPLGSTELELNAQPGN
                     VQLVDNKGQRYTADDAEEMIGKLTGMPIPLNSLRQWILGLPGDATDYKLDDQYRLSEI
                     TYSQNGKNWKVVYGGYDTKTQPAMPANMELTDGGQRIKLKMDNWIVK"
     gene            41570..42421
                     /gene="ispE"
                     /locus_tag="NBI74_000937"
     CDS             41570..42421
                     /gene="ispE"
                     /locus_tag="NBI74_000937"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HCD1931307.1"
                     /translation="MRTQWPSPAKLNLFLYITGQRADGYHTLQTLFQFLDYGDIISIE
                     LRDDGDIRLLTPVEGVEHEDNLIVRAARLLMKTAADSGRLSTGSGANISIDKRLPMGG
                     GLGGGSSNAATVLVALNHLWQCGLSMDELAEMGLTLGADVPVFVRGHAAFAEGVGEIL
                     TPVDPPEKWYLVAHPGVSIPTPVIFKDPELPRNTPKRSIETLLKCEFSNDCEVIARKR
                     FREVDAVLSWLLEYAPSRLTGTGACVFAEFDTESEARQVLEQAPEWLNGFVAKGVNLS
                     PLHRAML"
     gene            42572..43519
                     /gene="prs"
                     /locus_tag="NBI74_000938"
     CDS             42572..43519
                     /gene="prs"
                     /locus_tag="NBI74_000938"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HCD1931308.1"
                     /translation="MPDMKLFAGNATPELAQRIANRLYTSLGDAAVGRFSDGEVSVQI
                     NENVRGGDIFIIQSTCAPTNDNLMELVVMVDALRRASAGRITAVIPYFGYARQDRRVR
                     SARVPITAKVVADFLSSVGVDRVLTVDLHAEQIQGFFDVPVDNVFGSPILLEDMLQLN
                     LDNPIVVSPDIGGVVRARAIAKLLNDTDMAIIDKRRPRANVSQVMHIIGDVAGRDCVL
                     VDDMIDTGGTLCKAAEALKERGAKRVFAYATHPIFSGNAANNLRNSVIDEVVVCDTIP
                     LSDEIKSLPNVRTLTLSGMLAEAIRRISNEESISAMFEH"
     gene            43644..45323
                     /gene="dauA"
                     /locus_tag="NBI74_000939"
     CDS             43644..45323
                     /gene="dauA"
                     /locus_tag="NBI74_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylic acid transporter DauA"
                     /protein_id="HCD1931309.1"
                     /translation="MNKIFSSHVMPFRALIDACWKEKYTAARFTRDLIAGITVGIIAI
                     PLAMALAIGSGVAPQYGLYTAAVAGIVIALTGGSRFSVSGPTAAFVVILYPVSQQFGL
                     AGLLVATLLSGIFLILMGLARFGRLIEYIPVSVTLGFTSGIGITIGTMQIKDFLGLQM
                     AHVPEHYLQKVGALFMALPTINVGDAAIGIVTLGILVFWPRLGIRLPGHLPALLAGCA
                     VMGIVNLLGGHVATIGSQFHYVLADGSQGNGIPQLLPQLVLPWDLPNSEFTLTWDSIR
                     TLLPAAFSMAMLGAIESLLCAVVLDGMTGTKHKANSELVGQGLGNIIAPFFGGITATA
                     AIARSAANVRAGATSPISAVIHSILVILALLVLAPLLSWLPLSAMAALLLMVAWNMSE
                     AHKVVDLLRHAPKDDIIVMLLCMSLTVLFDMVIAISVGIVLASLLFMRRIARMTRLAP
                     VVVDVPDDVLVLRVIGPLFFAAAEGLFTDLESRLEGKRIVILKWDAVPVLDAGGLDAF
                     QRFVKRLPEGCELRVCNLEFQPLRTMARAGIQPIPGRLAFFPNRRAAMADL"
     gene            complement(45378..45656)
                     /gene="ychH"
                     /locus_tag="NBI74_000940"
     CDS             complement(45378..45656)
                     /gene="ychH"
                     /locus_tag="NBI74_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress-induced protein YchH"
                     /protein_id="HCD1931310.1"
                     /translation="MKRKNASLLGNVLMGLGLVVMVVGVGYSILNQLPQFNMPQYFAH
                     GAVLSIFVGAILWLAGARVGGHEQVCDRYWWVRHYDKRCRRSDNRRHS"
     gene            45934..46518
                     /gene="pth"
                     /locus_tag="NBI74_000941"
     CDS             45934..46518
                     /gene="pth"
                     /locus_tag="NBI74_000941"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HCD1931311.1"
                     /translation="MTIKLIVGLANPGAEYAATRHNAGAWFVDLLAERLRAPLREEAK
                     FFGYTSRVTLGGEDVRLLVPTTFMNLSGKAVAAMASFFRINPDEILVAHDELDLPPGV
                     AKFKLGSGHGGHNGLKDIISKLGNNPNFHRLRIGIGHPGDKNKVVGFVLGKPPVSEQK
                     LIDEAIDEAARCTEMWFTDGLTKATNRLHAFKAQ"
     gene            46635..47726
                     /gene="ychF"
                     /locus_tag="NBI74_000942"
     CDS             46635..47726
                     /gene="ychF"
                     /locus_tag="NBI74_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HCD1931312.1"
                     /translation="MGFKCGIVGLPNVGKSTLFNALTKAGIEAANFPFCTIEPNTGVV
                     PMPDPRLDQLAEIVKPQRTLPTTMEFVDIAGLVKGASKGEGLGNQFLTNIRETEAIGH
                     VVRCFENDNIIHVSGKVNPADDIEVINTELALADLDTCERAIHRVQKKAKGGDKDAKA
                     ELAVLEKCLPQLENAGMLRALDLSAEEKAAVRYLSFLTLKPTMYIANVNEDGFENNPY
                     LDQVREIAAKEGSVVVPVCAAVEADIAELDDEERDEFMQELGLEEPGLNRVIRAGYKL
                     LNLQTYFTAGVKEVRAWTIPVGATAPQAAGKIHTDFEKGFIRAQTISFEDFITYKGEQ
                     GAKEAGKMRAEGKDYIVKDGDVMNFLFNV"
     gene            47941..49116
                     /locus_tag="NBI74_000943"
     CDS             47941..49116
                     /locus_tag="NBI74_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001357423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
                     /protein_id="HCD1931313.1"
                     /translation="MLTDTQCRTAKPKEKLYRLNDFNGLYLEVKPNGKKAWRYRFKLN
                     GKSSMFALGEYPTVKLAEAREKCEQARKQVADGVSPTQARQLDKIRKVNDASNTFELI
                     AKEWLQMKDWAGITKTRRLDMLERVVFPAIGKLPVREITPHHILKILQETAKRGAPTV
                     AAEARRTISSVFELAVATLRADSDPVWPVRKALPANKTQHKQALNPQQIGKLLSCFDN
                     SRGSYQVNYCMWLMWWTLARPAEATEAEWTEFNLDNALWTIPAARMKARREHVIPLPF
                     QAVKMLRTLQGLTGHRQHLFPGRDNPLGPMTSHSLRQLLKSLGWSGTYSPHATRTTGS
                     TRLNELGYRPDAIEAQLAHADTNNVRRTYNHATYLDERKVMMQEWADMLDKWVAGFK"
     gene            49227..49511
                     /locus_tag="NBI74_000944"
     CDS             49227..49511
                     /locus_tag="NBI74_000944"
                     /inference="COORDINATES: protein motif:HMM:NF013677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HCD1931314.1"
                     /translation="MGTPRFTPEFKEEAVRQVTERGYSVADVSERLGVSAHSLYKWVR
                     SVRPDNSGHQAQDLLDARTEILRLKAQLKRTEEERDILKKAARYFAREPD"
     gene            49508..50362
                     /locus_tag="NBI74_000945"
     CDS             49508..50362
                     /locus_tag="NBI74_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011871062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HCD1931315.1"
                     /translation="MKYRFINDHREIWSIVTMCRVLKVARAGFYVWLHRPVSAGEKDN
                     QRLLELIRDAYALSGGVYGYRRVHGDLREIGEVCSRNRVARIMKRNRIQAVHGYKVPR
                     GVRGRPSLIAPNRVQREFTMVKPNQVWVTDITYIRTWQGWLYLAVVIDLFARNVVGWS
                     MKPTLSRELALDALLMAVWRRKPAENVIVHSDQGSQYGSDDWQRFCRANNLAPSMSRR
                     GNCWDNAVAESFFSSLKKERIRKRIYKTRDMARADIFDYIEVFYNRTRRHSHLGGVSP
                     EAFEKASP"
     gene            complement(50985..51341)
                     /locus_tag="NBI74_000946"
                     /pseudo
     CDS             complement(50985..51341)
                     /locus_tag="NBI74_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016243248.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
BASE COUNT        13068 a        13459 c        12645 g        12408 t
ORIGIN      
        1 tccttttgct tttgataact gcgcaggaac aaagtaaaaa taatgacgaa gtaaacggag
       61 catggcagct ccgtttcatt tgaaaggaat taattaagca ggccggggaa aatcccctta
      121 ataccagtaa cgataaattc aatccccaac gccatcagca atagccccat aatacgcgta
      181 atcacgttga tgccggtctg gcgtaaaact cgtaccagcc acggtgccat gcgaaacaat
      241 ccccagcaac ataaagcgaa caatgcaata gccacaaaga aaccaaacag atagctaatg
      301 ctgtgataac gcgtacccca gacgatggta gaactgatcg cccctggccc cgccatcaac
      361 ggcaacgcca atggcaccac accaatgctt tcacgcaccg cggtttctga tttttcttgc
      421 ttgttctgtt tatcctcacc aagcttgccg ctgatcatcg acatcgctat tgtcaccacc
      481 aggatgccac cggcgatacg gaatgaatca attgatatac caaaaagttg tagaatcgtg
      541 tcgccgagaa aaagcgagat ccacaagata atggccacag acaggttggc tgtaaggtta
      601 gttttgtttc gcgctgccgc tgtctgataa ctggtcatgc tgataaagac gggaataatc
      661 cctaccgggt tgaccagcgc aaataacccg atgaaaaatt tgaagtaaac gggaaaatca
      721 aaaaaggtct gaatcacggt tagctccgaa gcaaaagccg gataatgtta gccataaata
      781 aggttgaaaa gacgcgctga caatacgcct tttgacagca tttttcacct cctaactact
      841 taaaattgct atcattcgtt attgttatct agttgtgcaa aacatgctaa tgtagccaac
      901 aaatcatact acaatttgtt aactgttagc tataatggcg aaaagccatg ctgaaaggtg
      961 tcagctttgc aaaaatttga tcttgatcac gtaatcagta cccagaagtg agtaatcttg
     1021 cttacgccac ctggaagtga cgcattagag ataataactc taatgtttaa actcttttag
     1081 taaatcacag tgagtgtgag cgcgagtaag cttttgattt ccatcggtta agcaaatcat
     1141 caccgcactg actatactct cgtattcgag cagatgattt actaaaaaag tttaacatta
     1201 tcaggagagc attatggctg ttactaatgt cgctgaactt aacgcactcg tagagcgtgt
     1261 aaaaaaagcc cagcgtgaat atgccagttt cactcaagag caagtagaca aaatcttccg
     1321 cgccgccgct ctggctgctg cagatgctcg aatcccactc gcgaaaatgg ccgttgccga
     1381 atccggcatg ggtatcgtcg aagataaagt gatcaaaaac cactttgctt ctgaatatat
     1441 ctacaacgcc tataaagatg aaaaaacctg tggtgttctg tctgaagacg acacttttgg
     1501 taccattact atcgctgaac ccatcggtat tatttgcggc atcgttccga ccactaaccc
     1561 gacttcaact gctatcttca aatcactgat cagcctgaag acccgtaacg ccattatctt
     1621 ctccccgcac ccgcgtgcaa aagatgcaac caacaaagcg gctgatatcg ttctgcaggc
     1681 tgctatcgct gccggtgctc cgaaagatct gatcggctgg atcgatcaac cttctgttga
     1741 actgtctaac gcactgatgc accacccaga catcaacctg atcctcgcga ctggtggtcc
     1801 gggcatggtt aaagccgcat acagctccgg taaaccagct atcggcgtag gcgcgggcaa
     1861 cactccagtt gttatcgatg aaactgctga tatcaaacgt gcagttgcat ctgtactgat
     1921 gtccaaaacc ttcgacaacg gcgtaatctg tgcttctgaa cagtctgttg ttgttgttga
     1981 ctctgtttat gacgcagtac gtgaacgttt cgcaacccac ggtggctatc tgttgcaggg
     2041 taaagagctg aaagctgttc aggacgttat cctgaaaaac ggtgcgctga acgcggctat
     2101 cgttggtcag ccagcctata aaattgctga actggcaggc ttctctgtac cagaaaacac
     2161 caagattctg atcggtgaag taaccgttgt tgatgaaagc gaaccgttcg cacatgaaaa
     2221 actgtccccg actttggcaa tgtaccgtgc taaggatttc gaagacgcgg tagaaaaagc
     2281 agaaaaactg gttgctatgg gcggtatcgg tcatacctct tgcctgtaca ctgaccagga
     2341 taaccaaccg gctcgcgttt cttacttcgg tcagaaaatg aaaacggctc gtatcctgat
     2401 taacaccccg gcttctcagg gtggtatcgg tgacctgtat aacttcaaac tcgcaccttc
     2461 cctgactctg ggttgtggtt cctggggtgg taactccatc tctgaaaacg ttggtccgaa
     2521 acacctgatc aacaagaaaa ccgttgctaa gcgagctgaa aacatgttgt ggcacaaact
     2581 tccgaaatct atctacttcc gccgtggctc cctgccaatc gcgctggatg aagtgattac
     2641 tgatggccac aaacgtgcgc tcatcgtgac tgaccgcttc ctgttcaaca atggctatgc
     2701 tgatcagatc acttccgtat tgaaagcagc aggcgttgaa actgaagtct tcttcgaagt
     2761 agaagctgac ccgaccctga gcatcgttcg taaaggtgca gaactggcaa actccttcaa
     2821 accagacgtg attatcgcgc tgggtggtgg ttccccgatg gacgctgcga agatcatgtg
     2881 ggttatgtac gaacatccgg aaactcactt cgaagaactg gcgctgcgct ttatggatat
     2941 ccgtaaacgt atctacaagt tcccgaaaat gggtgtgaaa gcgaaaatga tcgctgtcac
     3001 caccacttct ggtacaggtt ctgaagtcac tccgtttgcg gttgtaactg acgacactac
     3061 tggtcagaaa tatccgctgg cagactatgc actgaccccg gatatggcga ttgtcgacgc
     3121 caaccttgtt atggacatgc cgaagtccct gtgtgctttc ggtggtctgg acgcagtaac
     3181 tcacgccatg gaagcttatg tttctgtact ggcatctgag ttctctgatg gtcaggctct
     3241 gcaggcactg aaactactga aagaatatct gccagcgtcc taccacgaag ggtctaaaaa
     3301 tccggtagcg cgtgaacgtg ttcacagtgc agcgactatc gcgggtatcg cgtttgcgaa
     3361 cgccttcctg ggtgtatgtc actcaatggc gcacaaactg ggttcccagt tccatattcc
     3421 gcacggtctg gcaaacgccc tgctgatttg taacgttatt cgctacaacg cgaatgacaa
     3481 cccgaccaag cagactgcat tcagccagta tgaccgtccg caggctcgcc gtcgttatgc
     3541 tgaaattgct gaccacctgg gtctgagcgc accgggcgac cgtactgctg ctaagatcga
     3601 gaaactgctg gcatggctgg aaacactgaa agctgaactg ggtattccga aatctatccg
     3661 tgaagctggc gttcaggaag cagacttcct ggcgaacgtg gataaactgt ctgaagatgc
     3721 attcgatgac cagtgcaccg gcgctaaccc gcgttacccg ctgatctccg agctgaaaca
     3781 gattctgctg gatacttact acggtcgtga ttatgtagaa ggcgaaactg cagcgaagaa
     3841 agaagccgct ccggctaaag ctgagaaaaa agcgaaaaaa tccgcttaat cagtagcgct
     3901 gtctggcaac ataacggccc cttctggggt aatgccagtc agttaaggat cagttgaccg
     3961 atccagtggc tgtgtaagaa tccggaaacg ctcactcgtt tccggatttt tttatgctca
     4021 ttggacaggc ccttgatttg gtatcccgtt acgattctct gcgtaaccca ctgacttctc
     4081 tgggggatta cctcgacccc gaactcatct ctcgttgcct tgccgaatca ggtactgtaa
     4141 cgctacgcaa gcgccgtctt cccctcgaaa tgatggtctg gtgtattgtt ggcatggcgc
     4201 ttgagcgtaa agaacctctt caccagattg tgaatcgcct ggatatcatg ctgccgggca
     4261 atcgcccctt cgttgccccc agtgccgtta ttcagacccg ccagcgtctg ggaattgagg
     4321 ctgtccgccg cgtgttcacg aaaacagcgc agctctggca taacaccacg ccgcatccgc
     4381 actggtgcgg cctgaccctg ctggccatcg atggtgtgtt ctggcgcaca ccggatacac
     4441 cagagaacga tgcagccttc ccccgccaga cacatgccgg gaacccggcg ctctacctgc
     4501 aggtcaaaat ggtctgccag atggaactga ccagccatct gctgacggct gcagccttcg
     4561 gcacgatgaa gaacagcgaa aatgagcttg ctgagcaact tatagaacaa accggcgata
     4621 acaccctgac gttaatggat aaaggttatt actcactggg actgttaaat acctggagcc
     4681 tggcgggaga acaccgccac tggatgatcc ctctcagaaa gggagcgcaa tatgaagaga
     4741 tcagaaaact gggtaaaggc gatcatctgg tgaagctgaa caccagcccg caggcacgaa
     4801 aaaagtggcc gggactggga aatgaggtga cagtccgcct gctgaccgtg acgcgcaaag
     4861 gaaaagtctg ccatctgctg acgtcgatga cggacgccat gcgcttcccc gggacgtata
     4921 ccggagctga tgcgcgatct tgcaagtatg ggacaactag tgaaattacc gacaagaaga
     4981 gaaagggcct tcccgagagt gataaaggag aggccctgga aatactccac agccccgaaa
     5041 aagagccagt cagttgctta actgactggc attacccctt ctggggccgt tttttatttt
     5101 cccaaagcaa cttttccata aaccggcagc attgtccttc atcatatttg cgacgatgta
     5161 taacgcctac acacagcgat attgtacttt acaggttaca agtcaatgtc ggtgtataag
     5221 cgctctgtga ggcgtataac ggcgttaaaa aactgccgag aagggtgtat agcccggaag
     5281 aagtgcgtaa aacgaactga cagggataaa agcgccctgc tcaccctgtc agtaaagaaa
     5341 ttcttattaa tcgtggcgat gcctttcctg aatagccgtt aatgagccga cttctaacgc
     5401 ctctttatag tgtttacggc atacagaaac gtagcgttca ttaccaccaa taaccacctg
     5461 ctcaccttcg ttataaggtc tgcctgcttg atcaagacgc agcaccatgc ttgctttacg
     5521 gccacaaaaa cagatggttt ttaattcaac cagtttgtcg gaccatgcca gtaagtattg
     5581 actgccaata aataattcac ctcgaaaatc ggtacgtaga ccataacaaa gtacgggtat
     5641 atcgagttga tcgacaacct ccgataattc atatacttgt tgtctggtta aaaactggca
     5701 ttcatcaacc agtacgcaat gaattgcctg ctgttcatgt tccgcacgaa tctcatcaaa
     5761 taatgatgaa ttttggttaa ataattttgc aggtgatgac aaacctatac gcgaactgac
     5821 tttcccggca ccaaagcgat catcaatttc tgccgtatat acgacagtgc gcatgccgcg
     5881 ttcctggtaa ttgtatgaag attgcaacaa tgctgtagac ttacccgcat tcattgcgga
     5941 atagtagaaa tatagctgtg ccatcagcca caggccctca atgatatgaa ataaagttgg
     6001 ctggagttta tcataattcg tcagtttttc agctcaacag tatgcacaga agtggtatat
     6061 gcgttctccc ttacgaagcc ttgcataatc cttctgagct atcattacaa ctgcctcgct
     6121 tgttataagc gggtaaatga ctgccggtaa ctattcacaa tctttaacct gttgcgcatg
     6181 taatagccct ctgttgacct ccaggagata gtgcaatact aagtccctgc tcttattgcg
     6241 actgttctac ttttcatcat tcgcttaata gggaattctc gtaaacacga ctaatacaga
     6301 agactgaaag gtcgtcagcc tacgattatc tccccataaa atgtgacatg aatcaggaag
     6361 ttttaacctc acgtgctgcg aaatcatcgg tgcaaatagg gctatatgcc gcgtcttttc
     6421 tggctaattt tatgaaaaga tatttattgg cggcacaaaa taaagaacaa ttttgaattc
     6481 cttacattcc tggctattgc acaactgaat ttaaggctct attattacct caacaaacca
     6541 ccccaatata agtttgagat tactacaatg agcgaagcac ttaaaattct gaacaacatc
     6601 cgtactcttc gtgcgcaggc aagagaatgt acacttgaaa cgctggaaga aatgctggaa
     6661 aaattagaag ttgttgttaa cgaacgtcgc gaagaagaaa gcgcggctgc tgctgaagtt
     6721 gaagagcgca ctcgtaaact gcagcaatat cgcgaaatgc tgatcgctga cggtattgac
     6781 ccgaacgaac tgctgaatag ccttgctgcc gttaaatctg gcaccaaagc taagcgtgct
     6841 cagcgtccgg caaaatatag ctacgttgac gaaaacggcg aaactaaaac ctggactggc
     6901 cagggccgta ctccagctgt aatcaaaaaa gcaatggatg agcaaggtaa atccctcgac
     6961 gatttcctga tcaagcaata atcttttgta gattgcacat gctaaaaatc ccgccatcgg
     7021 cgggattttt tattgtccgg tttaagacaa tttaataaga ataagctata aaaaaaacgg
     7081 cgtcgattgc tcaacaccgt ttcgtggaaa acaccgatac ggatgttact tcttaatgcc
     7141 catctcttct tcaagccagg ctttaaattc cgtgccaagg gtgttatgac gaataccgta
     7201 ttcaacgaag gcctgcatgt aacctaattt attaccgcag tcatggctct tccctttcat
     7261 atgataggct tcaaccgttt ctttttcgat cagcatatca attgcgtcgg tgagctgaat
     7321 ttcatcacca gctccaggag gggtttttgc cagcaacggc caaatatccg cgctaagtac
     7381 gtaacgaccc acaatagcga gattagacgg cgcaacatcc gcttttggtt tttcaaccac
     7441 gccaaccatc ggtacgcttt cacccggcgc taattcaacg cctttgcaat ccacaacgcc
     7501 atatgcggtc acatcagcaa ccggttcaac catgatctgg ctatgacccg tttcatcaaa
     7561 gcggcggatc atctctgcca ggttatcctg tgataaatcg gattcatatt catccagaat
     7621 aacgtcaggc aaaataacag ccacaggttc atcacccact accgggtgag cacacaatac
     7681 cgcgtgtccc aggcctttcg ccagcccctg acgaacttgc ataatagtca cgtgcggcgg
     7741 acaaatagac tgcacttcat caagcagttg acgttttaca cgtttttcca gcattgcttc
     7801 cagttcaaaa ctggtatcaa agtggttttc aatagagttt ttagatgagt gagtaaccag
     7861 cacaatttca gtaatgccag ccgcaataca ttcattcacg acgtattgaa ttaatggctt
     7921 atcgacaagt ggcagcatct ctttcgggat ggctttcgtc gccggcaaca tcctggttcc
     7981 taatcccgct acggggataa cggctttttt gactttcgta ttaatggcag ccatttaaat
     8041 tctcctggac tgttcgtgtt ttgaacgtgt tcatatttct gtatcgcatc ccagtatatc
     8101 agcaccccga aggggctaag ttctgaaaag gaggcgagtt agcataatta agacaattac
     8161 ttatacgaat agcgacgata gtaccaccag ccgacattag caggtaatgc aaatttagcc
     8221 cgtgttatcg tttgctcatt ctgcagacaa catcaagcgc agccgaccac cggttcccca
     8281 tatttggcat tgccaggcat cgcatcgctg gctcaattga ttcaaataag cgttacctaa
     8341 agtgcctaac ggaacaccat tactgatttg cacctgatgt tcgccggtat ttaacgtcgc
     8401 attcagaccc gcagaaacca gaatgagatt tttcagttcg cgatgataat agccaactaa
     8461 tagcggaaac tgccccggca gattggcctg acggagtaaa tggtttacct gcttcaataa
     8521 cgcgcccaac tctggcaacc gttgattttg gtgtgcaagc tgttcctgta ataatccgtt
     8581 aaacaatgcg cgtaataaca aggcagcaag tacgccatta tgtccagctc gggtgacatc
     8641 aaggcaataa aatgccagat cgttttccga aagtgcggca atatcaagca ccaggccggg
     8701 tttgtccgcg gcaactaatt gacgataatt aacccggcat tgggaaatca cctgctgaac
     8761 cggcggttgt agttcctgta ataatttcgc cgctgcagca gggttatcaa ccattgcatc
     8821 ccagtcgcga aaaagccttt cctcttcctc aacgcgggaa ttaaacatgc tgggatagag
     8881 acaggcaaaa accatctcgc gcaagcgatt cagatcttta accggtttca gcaaaacatc
     8941 ttcaacgccc agacgtaacg ctttggcaat atctgccata ttttcagtgg cagatatcac
     9001 cagaactggt gtctggtcac ctctgttgcg tatatgttcc agcagtttaa gcccgttcat
     9061 tcgtggcatc gcgatatcac atatcatcag gtctggagtg aaacctccca gcaactcaag
     9121 ggcatccacc ccatcagccg ccagtaccgt tgtcgctccc aatgaggaaa accacgaatc
     9181 cagaagcgag cgaaatacct gctcatcttc aacaatgaga atctgttttc cgaccaatgg
     9241 ctgcgtcatg ttctctcccc tgactggctt tactcaatag tggcatgcta ttgccaccta
     9301 cgcctgtcag aattaactta agtgtagata aaaattctgg tcaaatgttg gtgcgtacca
     9361 acggcaaaag ttcgtccatt ttcttttcca ctgccagctg tccggccgca atggcagcgt
     9421 gcgcgcgatg gaaatcaagc gtagatattt gcgggcaaac aggttgaatc agaatatcgg
     9481 gcggatcacc tgccatgcgg ttccttttaa ggcggttctc cagcacctgg atagaagtgg
     9541 tcataatctc tgttgccgtt ggcgctgtca ccgcccgacg tgtcgttatg ctgcctaacc
     9601 tttctttcag acgcgcatgc cacggcagag aatcaccatt ctcgctattt tcttcactga
     9661 cattaaagga gagcaaatct tgttgcatca aatgagcatc gtgctgcagg tcgaccgcta
     9721 tcacaatatc agcccccaat gcacgcgtga gggaaatagg aattgggtta actacagcgc
     9781 catcaaccag ccagtagccg ttatgcgcaa cgggtgccat tagtcctgga atactgcatg
     9841 atgcacgaat agcaagatgg agatcgcctt cagtaaacca taattcacgc ccagtactta
     9901 aattggtggc aacagccgcg aagcgacggg aacaattttc gatctctgtt tccggcatta
     9961 tttcgcgata ttgattgaag acacgctcgc cgcgcagtaa cccaccgcgc tgccaggaga
    10021 gatccatcag gcgtaaaaca tcccaataac tgaaagaggt cacccaatct tccagcgcag
    10081 ataatcgatc gcatgcatag gcagcgccca ccagcgaacc aattgaacat cctgcaacga
    10141 tatcaatttc aatacccact ttttttagcg cattaattac gccaatatgc gaccaacctc
    10201 ttgccgcgcc agatcccagc gccagcccta tttttatctt tctcattatt cccgcaagat
    10261 tcccctggaa tgcaatcgta gccacatcgc tactgctcgg ttaacatagt gctactcagg
    10321 cggttaatac gccgttattg tttccaggga gattatttgt gtctcagctt tgtccctgtg
    10381 gtagtgctgt cgagtatagc ctatgttgcc acccttatgt gtctggtgaa aaggttgcac
    10441 ctgatccaga acatctcatg cgttcgcgtt actgcgcttt tgtgatgcaa gacgcagatt
    10501 atttaataaa gacctggcat ccatcttgtg gagcagcggc gttacgtgcc gaattgattg
    10561 ccggatttgc gcatactgaa tggttgggat tgaccgtatt tgaacattgc tggcaggacg
    10621 gcggcgatat cggatttgtc agttttgttg ctcgatttac cgagggtggc aaaacaggtg
    10681 caataattga acgttcgcga tttttgaaag aaaacggtca atggtactat attgacggta
    10741 cacgtccgca gtttggccgc aacgatccct gcccttgtgg ttcaggtaaa aaatttaaaa
    10801 agtgctgcgg ccaataatgg ttgacggtac ggtttagcaa acactctcaa caaggttttt
    10861 ccagcaatgc attcactcca acgtaaagtt ctgcgtacta tttgtccgga ccaaaaaggt
    10921 ctgatcgcac gtattaccaa tatttgctac aagcacgagt taaatatcgt gcagaacaat
    10981 gaatttgtag atcatcgtac cgggcgcttt tttatgcgca cggaactgga agggattttt
    11041 aatgattcca ccctgctggc agatctcgat agcgcattgc cagaaggctc cgtgcgtgag
    11101 ctgaatcctg ccggtcgtcg ccggatagtg attctggtca ctaaagaagc acattgcctt
    11161 ggcgatttgc tgatgaaagc caattacggc ggcctggatg tcgaaatcgc ggcagttatt
    11221 ggtaaccacg ataatttacg ttctctggtt gagcgttttg atattccgtt tgagctggta
    11281 agccatgaag ggttaagccg caacgagcac gatcaaaaga tggcggatgc cattgatgct
    11341 tatcaacctg actatgtggt gctggcgaag tatatgcggg tattaacgcc ggaatttgtg
    11401 gctcgcttcc cgaataagat catcaatatt caccattcct tcctgccagc gtttatcggc
    11461 gcacgtcctt atcaccaggc ctatgagcgt ggcgtgaaga ttattggcgc aaccgctcac
    11521 tatgtgaatg acaatctgga cgaaggtcca atcatcatgc aggacgttat tcatgtcgat
    11581 catacctaca cagctgaaga tatgatgcgc gcaggtcgtg acgtcgagaa aaacgtctta
    11641 agtcgtgcgc tatacaaagt actggcgcag cgcgtctttg tttacggtaa tcggacgatt
    11701 attctttaat cgtcagccaa aataactggt tagctttaat ccgttacgga tgaaaatcac
    11761 gcaaccagtt catttttctc aacgtaacac tttacagcgg cgcgtcattt gatatgatgc
    11821 gccccgcttc ccgataaggg agcaggccag taaaaagcat taccccgtgg tggggttccc
    11881 gagcggccaa agggagcaga ctgtaaatct gccgtcatcg acttcgaagg ttcgaatcct
    11941 tcccccacca ccatctcaag cattacctac aatatgaatt acccctggtg gggttcccga
    12001 gcggccaaag ggagcagact gtaaatctgc cgtcatcgac ttcgaaggtt cgaatccttc
    12061 ccccaccacc atcactcgat tccattatca aaccttcggc ttctccccca ttcgccgtaa
    12121 aaactgctgc tgtagcctca acaaatccgc taacgtcaac aactgataat ccagaccttg
    12181 ctggctggca atttgcgcaa taatgcggct tagcgccgaa taatggcgat gatgccagtg
    12241 aggaaacaga tgatgggtaa ggtgcagatt aatcccaccc agccagtagc caagccatgc
    12301 aggttgcggc gtccaatcaa aggtggtagc aaaggtatgc gccagtctgc ccacgggcat
    12361 tttgccctct tctggtggta actgcgtatg tcccttcgcc cagtgcgttc caataatcaa
    12421 cgtcacaaaa atcaatgacg ccagcagctg gctgagcaga taagtcagca ggatggtcat
    12481 aaaactaaaa tcagacaaaa gccagggaat tatcagacac agcgcacaat gcgccagttt
    12541 cccggctaag aaatatcccc agccagcaaa gcctcgcagg gccagatgtc gcgtgaccgg
    12601 cgtaaaccct gcgcgatcga gccagtccac tacccagata taccagggaa aggtgagcgc
    12661 ggcgaccagt ggccaatagc gatgttgctg gcgcataaaa ggtcgccacc gttgccacgg
    12721 cgtctggcga agaatgccat tttctgctgt atctggatcg taaaattcaa tattggtaaa
    12781 accgtgatga aagcgaacgt ggcgcacccg ccagcagtcc ggatctagcc ccataggaaa
    12841 agcaacccac cggttaagcc gggcattggt ctttttacca cgcaaaaacg catcatgcga
    12901 agcgtcatgc accacattga ccgctaacaa catggcgcaa aacatcatcg cgagatacca
    12961 caacagatac agtccccatt gacgttgaaa taacgccagt aagtaacagg agaaacaagc
    13021 aataatcaac accgccattt tgacgaatgc ccatccgtcg gcataccgat gatcgcctga
    13081 tttcgccaga taaactttcg taccgctttg cagttgtttc accagttgtg gatcatcggc
    13141 aggaaaagtg attaacctgt cgggattaaa caggttcata ctgtttcctt agcgatttac
    13201 gtcctacgcc gattacaaat aacaggcaat gggccagcag aatacccact aatcccttcc
    13261 agagcagagt actccatgaa cacatataga caataaaata gagcagtgac aacgtcacag
    13321 agcaccacaa aatcataccc caatgctgcc cgtcgctcat gccccccagc gcaacggagc
    13381 ctgccgccag cagaacaaat aatacggctt gctcgctggt aataaactca atgccatagc
    13441 catattgttg aatgtagcca agcaccagca taaacagcag gatcacccct gcccctaccg
    13501 cccatggaga actctggtag cgaccttcaa tcgctttgcc aaatgagcaa aagcccatta
    13561 agcgtaaaaa tggcagatta ctctcgcgca tcgggttaca ggacaagcga ctatttttta
    13621 cgccgtagcc tacgtcgtat tccggcccgg cactatacga accgaaaagt ttatcccaca
    13681 acaaaaaggt gccaccgtaa ttggtatcgg catattgctt ttgattcaga tgatggacgc
    13741 gatgatgcga aggcgtgatc agcacttttt ccagccagcc cagtttcggc gttagtgcgt
    13801 tatggttaaa aaattgccaa ctatagtgaa taatagaaac cgccaaaaag acctgtaacg
    13861 gcacaccaag catcgccagc agcataaaaa acgggattga ggttaacgag gagtaccacg
    13921 agttccgcac gcctaatgac aggttgtagt gttccccctg atgatgcacc acatgtaccg
    13981 cccacagcgg gcgcatttcg tgatgccagc gatgtagcca gtaaaagccg aaatcccacg
    14041 ccaggatagt aaacaaccac agcaggatca ccggcacatt atcaaacagt ccaaggctga
    14101 agtgattgac caccaggcca tagcagagca cttccaggca gcgaaataac cacagcatca
    14161 tatgaccgga gttgatatta aaaataacgt ccagccagtc cactttttgc cgcccggtga
    14221 attgcaacac gcccgcttcg gctaacacca acagcaccat cgatatgatg gggaataata
    14281 aatcactcat attgtgtctt ccttttctcg catgttgcgc atatccaacc gcaaatcgac
    14341 gccagcaacg cgccgcctac taaatcgata aataaatgtc tgcgtaattg cagaatcgaa
    14401 aaggcgattg ctatcgccca gaccacgctc attagcgtca gccaacggtt ccccttttta
    14461 gcaatcgccc ataccgccaa cagcgttagc gcggcgtgta aggagggaaa acagttctgg
    14521 cttgagtcaa tggcgatcag cctgttgagt gcgcccgcgg atatccccat ctgatcaaac
    14581 gagggataat gcatggttgt gggccagaga agataaaata cgccagccac cagtgcggaa
    14641 atttgcattg aacgggccag ccagcgcagg tggcaaagtg gcgtggataa ataacctaac
    14701 ggcacaatga aaaaaaacga gagatacagc cacacagcgg agggggaaaa cgcaatcaaa
    14761 ttatcaatta ttgacggtgt taattgatat ccctcacctt gcaggtagtc gctaaagttg
    14821 tagataaccc caacagttcc ccagcccagc agcatctgtt tcaggcgtag cagtgaattg
    14881 ctcattcccg cccccactga cgcacaatgc gccgccgctt ggctgtaaaa gagactggca
    14941 cagtttgcag ttccagcgac caaattaatt gcccgacagc gatatcctgg cgtataaaca
    15001 aggcattcaa cgcctcccgg caatgctcca gcatccacag ctcgcaatcg gcgattaacg
    15061 tcaattgtgc tgacccggtt tgtagcaggc gataatcgac tgttagcggc agtgtcattg
    15121 ccagcacacg actacaggca tcggcaaaaa cggtctgaat atcgccggat cttgttggta
    15181 gttgcagttg gtcatcctgc cgcccttcga tacgcgcaat cgccattgtt gcactgccac
    15241 aggggcatgg ctgttcgctt gccaccagca catcatccag tcgataacgc actatcggct
    15301 gtgtggtgcg ggtaaagtct gtgacgatcg gcacgaaacg gtgctcatcc agccattgcg
    15361 gttcaatgtg aacaaactct tcgttgagat gtaacgttcc acaccggcag gtggaagcga
    15421 gaaagccttc tgtcgcctga taaatttccc ccacattgtg gaacacgttg cgcagtaatt
    15481 cccgatcctg cggctccaga acttccgcca ccgaaatcac ttttttgacc tttagcgtca
    15541 attcgccggc catcacctcc agcgccagtg cgcgtaatac ctgagctggc gcgacaataa
    15601 tcgtgggcgc tcgctgttgg agaagtttga gttgcgcctg aaatggcgct agcagatcgt
    15661 aaaaatcgag acttaaccag cgattattca cgctttgata caggttgttg tccgcgcgca
    15721 gaaacagcgc cacccgctct ttggcaaata aatcatcggg caacactttt gccagcacac
    15781 cggcggccca catctgttgc tcgtgcgggc taacaacaaa aagtccacga cgcccggatg
    15841 tacctgatga aagcccaacg ctgaacttac caacgcaggg tttgaaatca cgactctgct
    15901 cactgcgcat tgcacaatcg agaagttcgt cgcgctttaa acctgcggta ttcatctcat
    15961 cgaagtgcgt catcattaac gctttatcca tcattggcca ctgattaaag ggcaacgtca
    16021 gataacgctg aaaccacggg ctatgagata agacattttg ccggaactga tgcagccttt
    16081 ttgcctgcca gttttccagt gcctcgcgat cggtgaaatg cagcctgcgg gtacggaagt
    16141 agcgccagat caacatcaac gggatcataa atccccctca tggcacaaac gaatctccac
    16201 cacaccgttt tgccaaagct gatggagttg cccaagcgtg tggtagtagg cgtggctgtc
    16261 ggacataacc agatgcgcca gtggtgaagg ccctcgcagc tcccggtagt tagctggcgc
    16321 ccatgccgca tcgccagcca gtaaagtcca gccgttgtcg gttaacacaa acgcgccaat
    16381 atgtccggca gcatgtcctg gaagcggcac gagaataatt tcgccattgc tttctggcag
    16441 caaatagccg cactcaaacg gtgccagttg cgcgggcaaa ttcaccgatg cgaaactttc
    16501 aataaagcgc agtgaggagt caaaattctc cgggattagc cctggcacaa aggcctggcg
    16561 cagcgcagtg acaccacgca gagttcttac ttgccgccag ccttcaccag aacagatgca
    16621 ggaaacatca gggaaatcac gcaggccagc aatatgatca gcgtgaaaat gcgacagaat
    16681 aatcgcatca acatctttcg ctgcgaggcc ggtggcagaa agttgtcgca ccagcgcctg
    16741 ttgcggatcg aaataaaccg gcgtgacctg gcgataaaga cgaaacacac cagattgcgt
    16801 gtagtgttcg aaccacgatg aatagcctgt gtcccacagc caccgtttgt cgcccacctc
    16861 cagcagccag gcgcgggcgg ggaatttgca cactttcagc cctgcccctt tcagcgcaat
    16921 acagctaata tgggtacacc agcccgtttc aaatgcggtt atttttgcca tgttccctca
    16981 gccattgtcc cgtgattgca ataccttgtt ccatcgaaaa gcgtgggcga tatcccagct
    17041 ctgtaatggc cttttgcgca ctcagcgtca tatcgaaaca gagtgttccg gcgctgtatc
    17101 gtgtgataac aggttcctga ttaacgcact tcccatacag ttccaggcca cgcgcgatca
    17161 gagaaagcag cgaccaggga acagcagcaa tgcggtaact caagcccagt tgctggtgca
    17221 gcaaggcgtc aagcatcacc gatagctgct gcggctggtg attgctgatg ttgtaaatac
    17281 tgcccgagcg taagccttca acgttcgttg ccagccgcat ggcatacacc acgttttgca
    17341 cgaacgtgag atccaataac gcgtcgcccc cgcgcggcaa acgtaagcaa ccgccgccct
    17401 gttgcaattg ctgcatcaga cgaggaacaa ttacctggtc atgggggcca aacaaaccac
    17461 ggggacgcaa aagaataaat ctcgtggcag aatatcgaga aacagcgcca gcaataacct
    17521 gctcagcggc atatttactg ctggcataat ggctggagaa tgctcgcgca agataagttt
    17581 ctggcaggtc atagtgatgt tgaaaatcaa agtaaaccgc aggcgtggag atatggataa
    17641 accggggaat attcagcctg cctgccgcct gtgccagagt gtgggtcact ttgacattgg
    17701 caagccagaa cgccgttttg tcgccccagg gcgaggactt tgccgcacag tgccagacgg
    17761 catcgcagcc gtccatcagg cgggcgcagt catcctcggt tgcctcggtt aaatcgagag
    17821 ccacaaactg tgcgcctgcg tttgtcaggc ttctgccaac ctcacaattt cgccctgttg
    17881 ccacaaccga ctcacccgat tgcaacaaat attgcacggc atttcgccca aggccgctgg
    17941 tcgcccctgt tacgaggatc ttcacggtaa aagcaccatg ccagccagcg ttagtcccgc
    18001 tgccgtgcca atcaacatta caggtttatc cgctgtgaaa cgaccgctaa tcactgcttc
    18061 atgcagcgcc gtggggatag aggccgcgac ctgattgccg taaaaacgat acacatcgat
    18121 gagcgcagat tccggcactc gcagccgttt acgcatatgt tcaagggaaa gatgactggc
    18181 ctgatgcgga acgaccgtgc cgatatcaga cagttgcagg ctgctttttt gcagcaaccg
    18241 agaaaaataa tcttcaataa gcgaagaagc aaaacgaaac agcggtttcc cctgcatatg
    18301 aaaaagaaag tcgctatcac tcacaccaga gcgaggatta cgccgcgtac cgccagcacg
    18361 aatttcacac agttcgctgc cttcgggata catttcgatc aggctagcga taatcccgct
    18421 gcgcccatcg ccccgctcaa caatcgcgca cgccgcgcca tcaccaaaaa ttaatgaaga
    18481 ctcttcatga tcccagtcga taccgcgtga agcaatatct gccgaaacga tggcaatgcg
    18541 tcgatatgct ccacagttta ataatccggc agcgacttca agagcggaaa taaagctcac
    18601 acagctactg ttgatatcaa acccggcaac gcctacgggc atggctgcgg ctttaagaat
    18661 atgaattgcg ctacagggta atgcctgtac ggcaatggcc gatgcacaaa ttagcagatc
    18721 aatatcagaa ggatttaagg ctgccgctac taacgcatta tgtaaagcct ctgccgccag
    18781 ttcggcctgg ctggcgtcgt tgcgggcaaa atagcgccag caaataccgg aacgtcgctc
    18841 aacatagcca actggcttat ttaactgaac atccagttgt ttagaagtga tttgatcagg
    18901 gggtaaggcg atccccgtgg cgattatttt taacgccgcg gagatcgtcg gttgattagg
    18961 tcgagccacg gtcagtgctc cttcactgct ccataggtgg accaatcata tagatgaagt
    19021 acgtttccct acaatccgaa aaatcggcag tgtgtaaccg ccgatgtttg gtttagataa
    19081 tgtttggttt agataatgtt tggtttagat aattagtgac gagcgcgcac cagctgatac
    19141 ttacgcgtca gatactccac cgggacactc cagatgtgta ccagacgcga gaacgggaac
    19201 agcaggaata acgtcatccc caacaccagg tgcagacgga agataaacgc cacgccatca
    19261 agatgttgag aagcgccacc gtggaaggtc actaccgact gcgcccagcc aaccagtttc
    19321 atcatctcgc taccgtccat atgctgagca gagaacggaa tggtcagcag gcccagcgcg
    19381 cactggataa cgagcagcga caggatcagg atatccgctc cggtagtcgt tgcacgcacg
    19441 cgtgggctga acagacggcg tttcaacaac agcacgccgc caatcagaca cagtacgccg
    19501 ctggcaccac cagcaaacat cgccattttc tgcttcactt caatcggcag ccaggcttca
    19561 tacatccagt gcggcgtcag cataccgaag aagtgaccga caaaaatccc cagaataccg
    19621 atatggaaca ggttcgacgc cagattcatc cctttgcgat ccagcatctg gctggacgcc
    19681 gcgcgccagg tatactgccc gtagtcataa cgcagccagc taccaatcag gaagaccgcc
    19741 ccggctatgt acgggtagat atcaaagaag aacatattca ggaattgcat tagtgctgtc
    19801 ctccggtggt gatattcaga tattgcggcg cgacggcacc ggcaaagcga cgctgatgag
    19861 cagtgattgc tgaatcaccg cagcctttgt cagcaaagaa tttaacctgc tcttcttccc
    19921 agactgcatc cagcgcctgc ggcgtatcgt cgcgcgcttc gtcggcaatt ttttccgcca
    19981 ctttgtcgct gtcgattgcg gtattcgcca gtttcagcag cagatcaaac agcacggcat
    20041 aacggctttc acgctgttgc agacgcgcac tcagcaatgc cagaatcggc gcaatatctt
    20101 tcaaaccctc cacggcttcg ctttgcggca gctgcgccag gtactccaga tacagcggca
    20161 gatggtcggg caattcgcgg ctgtttaact gcaagccgtg ctgctcgtac tgcgccagca
    20221 gatccaccat cgcctggccg cggtcacggg attcaccgtg aacatgttca aatagcaaca
    20281 gtgacgtggc gcggccacgg tcgaacagtt cgctgtactg cgcctgggca tcgagcggat
    20341 ccatcgtcgt taaatcgcgc aggaaaatgc ccagcgcatg ggcatcctct tttggcagat
    20401 ttttcgacgc ggcaatcgcc tcaaacatct cttgttgatg ctgccataag gcagcatccg
    20461 gatattcaag gagacgcgat acaatcacga gttcgatcat gggtgcggct ccgttttgct
    20521 ggttacgtcg atggcatcga tacggcggct gttgaacaga ttgaatttgg tatctgaacc
    20581 gtggcagcca tcaccaaagg taaagccgca gccatttttc tccgggaagg cttcccgcgc
    20641 cagttcacga tgactgctcg gcaccacaaa gcgatcttcg tagttggcaa tagccagata
    20701 acgatacatc tcctgtgcct gggcttcggt cagaccgacc tcttccagcg cacgggtatc
    20761 gactttaccg tcaacggttt cagcacgttt gtaatgacgc atcgccagca tacgtttcag
    20821 cgcgcgcagt accggtttgg tatcaccggc ggtcagcaga ttcgccagat actgtaccgg
    20881 aatacgcagg ctttcgacgt ctggcagaat gccgttgctg cccagttcac ccgcgtctgc
    20941 cgcagactga atcggcgaca gaggcggcac gtaccagacc atcggcaatg tgcgatattc
    21001 cggatgcagc ggcagcgcca gtttccattc catcgccatt ttataaactg gcgactgctg
    21061 cgcagcctca ataacgctca gcggaatacc gtctttaatc gcctgctcga tgactttcgg
    21121 atcgttcgga tcgaggaaca catccagctg acgctggtag agatctttct cgttctcggt
    21181 acttgccgca cgttcaatcg cgtcggcatc gtacagcagc acgccaagat aacggatacg
    21241 accgacacag gtttctgaac atattgtcgg ctggccggct tcaatacgcg gatagcagaa
    21301 gatgcacttc tcagacttac cgctcttcca gttgaagtag atttttttgt acgggcatcc
    21361 ggtgatgcac atacgccagc cacggcattt atcctggtcg atcaggacga tgccgtcttc
    21421 ttcacgcttg taaatcgcac cgctcgggca ggtcgccaca catgccgggt tcagacagtg
    21481 ttcgcacagg cgcggcaaat acatcatgaa ggtgttttcg aactggctat acatcgcctt
    21541 ctggatgttg tcgaagttct tgtctttcgc cagcttgtca aactcaccgc ccaggtcatc
    21601 ttcccagttc ggcccttttt cgattttcgc catccgttcg ccggtaatca gcgaacgcgg
    21661 acgggcaatc ggctgtgatt tgctgccttc cggcgcggta tgcaggttct gataatcaaa
    21721 atcaaacggc tcgtaataat cgtcgatccc cggcagatgc gggttagcga agattttacc
    21781 cagcagcatg gcacggttac ccatgcgcgg ctgcagtttg ccgttgattt tacggatcca
    21841 gccgcctttg tatttttcct ggttttccca gtcagtcggg aagccctggc ccggcttggt
    21901 ttccacgttg ttgaaccacg cgtattccac gccttcacgg ctggtccaga cgtttttaca
    21961 ggtcactgaa caggtgtggc agccgatgca cttatcgaga ttcagcacca tgccgacttg
    22021 tgaacgaatt ttcattttac gctctcctgt acctggtcat tgccttcgcc atctaaccag
    22081 tcaatgttct tcatcttacg cactacaaca aactcatcgc ggttagaacc tacggtgccg
    22141 tagtagttaa agccatatgc cagatgggca tagccgccga tcatatgcgt cggtttcggc
    22201 gtgatacggg tgaccgagtt atggatacca ccacgctgtt gggtaatttc cgaaccaggc
    22261 aggttaacga tacgttcctg cgcgtggtac atcatggtca tccctgccgg aacacgctgg
    22321 ctgacaaccg cacgggcagt cagtgcaccg ttgctgttga agacttcaat ccagtcgtta
    22381 tcggcgatac ccagctcttt ggcatcggct tcactcagcc agaccaccgg accaccgcga
    22441 cctaaagtca gcatcaacag gttgtcgctg taggtggagt ggatacccca cttctggtgt
    22501 ggcgtcagga agttgagcgc tttttccggg ttgccattgg atttctggcc catcacttct
    22561 ttcaccgaac gggtgtcgat cggcggacga taaaccagca ggctttcacc gaaatcacgc
    22621 atccattggt gatcctgata cagctgctga cggccagaga gcgtacgcca cgggatcagc
    22681 tcgtgaacgt tggtgtaacc ggcgttgtaa gaaacgtgtt catcttcaag gccagaccat
    22741 gtcgggctgg agatgatttt gcgcggctgt gcctgaatat cgcggaagcg aatcttctcg
    22801 tcttctttat tcagcgccag atgcgtatgg tcacggccgg taaactcgct cagggcagcc
    22861 caggctttta cagctacctg accgttggtt tccggtgcca gcgtcaggat catctctgcc
    22921 gcatcaattg cggtattgag catcggctga ccttttgccg gaccttccgc ttttgtgtag
    22981 ttgagcttac gcagcagatc catctcgctc tgggtgttcc aggcaatccc tttaccgccg
    23041 ttaccgattt tctccatcag cgggccgata gaggtaaagc gttcgtaagt tgccggataa
    23101 tcgcgctcta cgaccataat gtgcggtgcg gttttacctg ggatcaggtc gcattcgcct
    23161 tttttccagt ctttcacatc cagcggttgc gccagttcag cggcagagtc gtgctggata
    23221 ggcagcgtga cgatgtcagt ttctttaccc agatgaccaa cgcacacttc ggagaatttc
    23281 ttcgcgatgg ctttgtagat ttcccagtcg cttttcgctt cccaggccgg atcgaccgcc
    23341 gcagacagcg ggtgaataaa cggatgcata tccgaagtat tcatgtcgtc tttttcgtac
    23401 caagtcgccg tcggcaaaat gatgtcggaa tagagacagg tgctcgacag acggaagtcc
    23461 agcgtcacca ccagatccag cttgccttcc agaccgttgt cctgccagtc cacttcttcc
    23521 ggcttcacgc cgccctgctg ccccaggtct ttgccctgga taccgtgctc cgtccccagc
    23581 aggtacttga gcataaactc atgaccttta ccggaagaac cgagcaggtt agaacgccag
    23641 atgaacaggt tacgcgggtg gtttttaccg ttttccggct gttccgcagc gaaacggata
    23701 gaaccctctt tcagggattt caccgtatag tccaccggat tcatcccggc tttttctgct
    23761 tcgcgagcga tagtcagcgg gttagtgcct aactgcggcg cagacggcag ccagcccatg
    23821 cgttctgcac ggacgttaaa gtcgatcaag tgtccggtat agcgagattt atccgccatc
    23881 ggcgacagca gttcttccgc agtcacggtt tcataacgcc actggctgga gtggttatag
    23941 aaataagagg tactgttcat gtgacgcgcc ggacgctgcc agtcgagggc aaacgccagc
    24001 ggctgccagc cggtttgcgg acgcagtttt tcctgaccta catagtgcgc ccagccgccc
    24061 ccgctctgac cgacacaacc gcagaaaatc agcatgttga tcagaccacg atagttcatg
    24121 tccaggtgat accagtggtt cagccctgcg ccgacgataa tcatcgaacg accgtgcgtt
    24181 ttatcagcgt tatcggcaaa ttcacgggcg atgcgaataa tctggctgcg agaaacgccg
    24241 gtaatctgct ctgcccaggc aggggtgtaa gctttcacat cgtcatagct ggttgcacag
    24301 ttaacgtcgt tcaggccacg ttccagaccg tagtttgcca gcgtcagatc ataaacggtg
    24361 gtcaccaggg cggtgctgcc atcagccagt tgcaggcgtt tcaccggcag cttatgcagc
    24421 agcacgtttt ccagttccac tttgttgaag tgttccgtgc catcgccacc aaagtacggg
    24481 aagcccacct cagcgatctc atcctggcta cccagcaggc tcagttgcag ctcggtttct
    24541 tcgccagttt tgccgtcgcg ctgctcaaga ttccatttgc ccttctcgcc ccagcggaag
    24601 ccaatagaac cgttcggcgc aaccatttca ccattggtat taaaggcgac agttttccat
    24661 tccggattgt tttcctggcc cagtgcatcc accagatcag cagcgcgcag catacgaccc
    24721 gcagcgtagt aaccgtcgcg ctcttccagc atcaccagca tcggcatgtc ggtgtagcgg
    24781 cgcacatagt cggtgaaata ctggcttggg ttgtcgaggt ggaattcacg cagcattacg
    24841 tgacccattg ccagcgccat tgccgcatcg gtgccctgtt tcggtgccag ccacagatcg
    24901 cacagtttgg cgatttcagc gtagtctggt gtgacggcaa cagttttggt ccctttgtaa
    24961 cgcacttcag taaagaagtg agcatccggg gtacgcgtct gcggcacgtt tgaaccccag
    25021 gcgatgatgt agctggagtt gtaccagtca gcagattccg gtacgtcagt ttgctcaccc
    25081 caggtttgcg gagacgcagg aggcaggtcg cagtaccagt cgtagaagct taagcaagta
    25141 ccgccaatca gcgagagata gcgcgcaccc gatgcgtaag aaaccatcga cattgccgga
    25201 attggcgaga agccagcgac acggtccggg ccgtaatttt tgatggtgta aacgttagat
    25261 gcggcgatca gttcgttcac ttcctgccag gaagaacgaa caaaaccacc gcgtccacgc
    25321 gcctgcttaa agcttttcgc tttatcggcg tcttcaatga tagaagccca tgcctcaacc
    25381 ggatcgctat gcagcgcctt cgcttcacgc cacattttca tcaggcgttt gcgcatcatc
    25441 gggtatttca ggcggttagc gctgtaaaga taccaggagt agctggcacc gcgcgggcag
    25501 ccgcgaggtt catggtttgg caggtccggg cgagtacgcg gatagtcagt ctgctgggtt
    25561 tcccaggtga ccagaccgtt tttgacgtag attttccagc tgcaggagcc ggtgcagttt
    25621 accccgtggg tagagcggac gattttgtca tgctgccaac gctggcgata tccatcctcc
    25681 cagtcacggt tggtattgag aagctggcca tgcccatcgg caaaggtttc acccttctgt
    25741 ttgaagtagc gaaaccggtc caggaattta ctcatcggtt ttctcctgtg ggagcctgtc
    25801 ggccctctga taaatcgaca ttgctaatgt ttaagggcaa ggtaacgctc tgaaacagcg
    25861 tgggaattga taacgatcaa gagtgatggg gatgaaaaat aaagtaaatt cctggaatac
    25921 ccctttagga taattaacat ttatagcata acagactgta aattaagacg ttttacacct
    25981 gtggttattt aaaccattaa gatttttatg cagatgggta ttaaggagta ttccccatgg
    26041 gtaacatatt aatgactctt aatgaaatcc agcgaaataa gccatacaac attagtatgt
    26101 tgtaactaaa tgacgcataa aaaaagcgcg acatcatgcc gcgcaaagga taatcaaata
    26161 ttacgattta ttttttagaa tgccgaccat ataccgccca ggtaatcacc acgcaggcga
    26221 tatagaaaat caaaaatact ttcattgcgc cgactggcga acccgttaat gccagcgagc
    26281 taccaaacgc tttcgggata aagaagccac caatcgcgcc aatcgcagag atgaaaccca
    26341 gcgccgccgc cgtgtcggtt gccgcttcac gcatcgcacg ttcgtcagaa cccccttctg
    26401 ctttcacgcg atccattgtc agtttacgga agatcacgga aatcatctgg aaagtggaac
    26461 cactacccag cccagcggtc aggaacagcg caaggaagac tgcgaagaac gccatgaagc
    26521 ttccgccctg tccgtcagtc ggtaaggtca ggaacagcag gccgctgaaa atcgccatca
    26581 ggataaagtt caccagcgtg acacgagttc cgcccagacg gtcagataat gcaccacctg
    26641 cagaacgcgc cagcgcacca ataaacggcc cgaagaaagc gtattgcaga atctgaacat
    26701 ccgggaactg cgtttttgac agcatcgcaa agcccgcgga gaagccgatg aaggagccga
    26761 aggttgccag atacagaagg ctcataatcc acagatgacc ccgtttgagt accggcaact
    26821 gctccttgat ggaggctttc gaggtagcaa gatcgttcat gccaaaccac gccgcaatgg
    26881 tgaagatggc aaggaacggc acccatatcc aggacgcatt cgccagatac agctcagtcc
    26941 catccggctg tttgacgccc tggctaccaa atactgcgaa aatcgacagt gataccacca
    27001 gcggagcaac caactgcatg acgctgacgc ccatgttgcc cagaccacca ttcagaccca
    27061 gcgcgccacc ctgcttctgt ttcggaaaga agaagctgat gtttgccata ctggatgcga
    27121 agttcgcgcc agcaaagccg cacagcagag aaatgatgat gaagacgcta taaggcgtgg
    27181 aggtatcctg cacggcaaaa cccagccaga cgcaaggaat aatcagaata ccggtgctga
    27241 acgccgtcca gcgacgacca ccaaagatag gaaccataaa ggagtatgga acacgtaata
    27301 acgcgccaga aaccgaaggc agcgcagtca acataaatag ctgatcggtc gtaaaattaa
    27361 aaccgacttt cggtaagttc accgcaacag cgctgaacaa catccatacg caaaacgcca
    27421 gcaacagaca gggaacggaa atccacaggt tgcggctggc aatacgttga ccgcgttgtt
    27481 gccagaacgc aggatcttcc ggtcgccaat ctgtaatgac agctccagta gccctttcgg
    27541 gggcggatga gtgactcata gacacctctg atactcgttt cgatgtctgc caccttagtg
    27601 tctgtagcta aaggcaattt gatgtaaatc aaacgataag ataactttat ctatcattga
    27661 tatttatcat tatccatagt gagtacagtg atttcataaa aattatgaga tttttcacgg
    27721 tactgtaaaa tccctaccct taccgatgta aagcgactaa ccacacggca aataaggagt
    27781 aactctttcc gggtatgggt atacttcagc caatagccga gaatactgcc attccagaat
    27841 gtatcgtcac attcattaag gttattgctc atttaaagcc tgaaggaaga ggtttacatg
    27901 cttaaacgtt gtctctctcc gctcaccctg gttaatcagg ttgcgcttat tgtgttgctt
    27961 tctactgcta ttggactggc agggatggcg gtttctggct ggctggtgca aggcgttcag
    28021 ggcagcgccc atgcgatcaa caaagcggga tcgctgcgca tgcaaagtta ccgtctgttg
    28081 gcagcagtgc cattaagcga gaaagacaag cccttaatta aagagatgga acaaacagca
    28141 tttagcgccg agttgactcg agcagcagaa cgagacgggc aactggcgca attacagggt
    28201 ttacaagatt actggcgtaa tgaactgatc cctgcactga tacgtgcaca aaaccgcgaa
    28261 acggtgtcag cggatgtcag ccagtttgtt gccgggcttg atcagctggt atctggtttt
    28321 gatcgcacca cggaaatgcg cattgagaca gtggtgctgg tccatcgggt aatggcggta
    28381 tttatggcac ttttactggt attcactatt atctggttgc gggcgagatt gctacaaccg
    28441 tggcggcaac tgctggcaat ggcgagtgcc gtcagccatc gcgattttac ccaacgcgca
    28501 aacatcagcg ggcgcaacga aatggcgatg cttggaactg cgttgaacaa tatgtctgca
    28561 gaactggcgg aaagttatgc cgtacttgag cagcgggttc aggagaaaac cgctgggctg
    28621 gagcataaaa atcagatcct ctctttttta tggcaggcta accgccgttt gcattcccgc
    28681 gccccgctgt gtgaacgcct gtcacctgta ctcaacggct tacagaattt aaccctgcta
    28741 cgtgatatcg aactgcgggt gtatgacact gatgatgaag agaatcatca ggagtttacc
    28801 tgccagtcag atatgacttg tgatgataaa ggctgccagc tctgcccgcg cggcgtatta
    28861 cccgttggcg atcgcggcac aaccctgaag tggcggctgg ctgacactca tacgcagtac
    28921 ggtattttgc tggcgaccct gccgcagggg cgtcatctta gccatgatca acaacaactg
    28981 gtggataccc tggttgaaca actcaccgcc acgctggcgc tggatcgcca tcaggaacgt
    29041 cagcaacagt tgatcgtgat ggaagagcgt gccaccattg cgcgcgaact gcatgattct
    29101 attgcccaat ctctctcttg catgaagatg caggtgagtt gcttacagat gcagggcgat
    29161 gcgctgccag aaagcagccg cgaactgtta agtcagatcc gtaacgaact gaatgcatcc
    29221 tgggtgcagt tgcgtgaatt gctcaccaca ttccgtttgc agctcaccga gcctggatta
    29281 cgtccggcgc tggaggcgag ttgcgaagag tacagcgcca aatttggctt cccggtgaag
    29341 ctggattatc aattgccgcc tcgtctggtg ccttcgcatc aggcaatcca cttgttgcaa
    29401 attgcccgtg aggcattaag taacgccctc aaacattcgc aagcgagtga ggtcgtggtg
    29461 acggtggcgc aaaacgataa tcaggtcaaa ctgaccgtcc aggataacgg ctgcggcgtg
    29521 cctgaaaatg ccatccgcag caatcactac ggcatgataa taatgcgcga ccgtgcgcaa
    29581 agtttacgag gcgattgccg cgtccgccgt cgtgaatcag gtggcaccga agtggtggtc
    29641 acctttattc ccgaaaaaac tttcacagac gtccaaggag atacccatga gtaatcagga
    29701 accggctact atcctgctga tcgacgatca cccgatgttg cgaactggcg taaaacagct
    29761 tatcagtatg gcaccagata tcactgtggt tggcgaagcg agtaatggcg aacagggtat
    29821 tgaactggcg gagtctcttg atcccgatct gatcctgtta gatctcaata tgcccggcat
    29881 gaacggtctg gaaacgctgg ataaactgcg cgaaaagtcc ctctccgggc gcattgtggt
    29941 attcagcgtc tctaaccatg aagaagatgt ggtcaccgca ctgaaacgcg gcgcggatgg
    30001 ctatctgtta aaagatatgg aaccggaaga tctgctgaaa gcgttacatc aggcagctgc
    30061 tggcgaaatg gtattaagcg aagcattaac gcctgttctg gccgccagct tgcgcgctaa
    30121 ccgtgccact actgagcgcg atgttaacca gttaacccca cgcgagcgcg atattctcaa
    30181 gctgattgcc cagggtttgc cgaacaagat gattgcccgc cgcctggaca tcaccgaaag
    30241 cacagtaaaa gttcacgtca agcacatgct gaagaaaatg aagctaaagt ctcgcgtgga
    30301 agctgcggta tgggtgcatc aggagcgcat tttctgatta cggttcccag cgcagttcgt
    30361 cgtttgacaa tgcgtcgaac ggttcgacaa gcgttagcgt gatctcattg gaggagacac
    30421 gctgcccctg gttatcttcc accactacag acaatcgcca gtgattgctc gccccttccc
    30481 cgttctgcca gtcaggcatg atcagcgtcc agccctcctc actgttggct tgtgcgcccg
    30541 gcgtcaaact taatatctgc gtatcgccct gccaaatcag ttgccgaata ccgtaacgac
    30601 tgcggatttg taatttcagc ggcactgttt cgccaggttt tagatcccac ggcggtgtcg
    30661 ccagaaacac cgttaacgtt tttcgctgtc ggtactcaag agtcggaaga ttatttcgct
    30721 gcggattgtc atagcgacta ccacgtaacg actgactttc gacaacctcg cccgcagaaa
    30781 gttgtttttt gagcggtaca ccaaagcggt agttaagatt cagcccgagg ttattttgat
    30841 tctcgccact ttcaccctgt ttatgctggg ccgtcacagt gactaagggc acaggggtgt
    30901 aatttaatcc cagactcaac gcgacgggat tgtgataacc cgtaccagag ttaaacaaat
    30961 cgacacgatc accaaaatac tgttctacgc tgacactggt attgaggtgt tgatagaacg
    31021 gcatgcgcat ccgggctgtc aggtcgtacc cgcgcgccat ccgttgttcc tgcgtggcag
    31081 tctgttcatg ccatgcagca aacggctgat aaaagtttgc cgatagtcgc aaatattcgc
    31141 cccacgcttc agcgccaaag cccgctcgct gaagattttc gtccagcaag ttgtcataaa
    31201 aagtgttata acccaccagc cagctgccgc gcgcccagcg ttgcccaacg cccacattgc
    31261 tcaccagccc atcatcctgc tgagtaagac caagctggct ccaggtgaga taacgatcat
    31321 tatcttgtaa cggcacaaac cagcttccac gactgccggt gaaatgtcct tcgttatcca
    31381 ctttgacgtc aacactggca tttccccacg gtgatagcca ggactctacg tgctgattaa
    31441 cctgttgact aagcgcatcg cggacttttc ccaatgcgaa agcttttgcc tgctcgccag
    31501 tatccagccc gttatcattc atactggttt cgccgaaatc tttcacaatt tcagcaaagt
    31561 gtttttcccc atcatgatgt tcaggtgcca tgcctaaatc cggcagacca tcattgctat
    31621 tctcaaagtg atttgccgct ttttgctcga aggtagattg tgcgttagct gtaccgcctg
    31681 ccgccagcaa gagtaaataa aacggaataa tgcgaggaac gaaacggctc aaaacgtcgg
    31741 taacttccgg taaaaatgaa aaaccatagc cttaaagaat aacgattact ggcacctttt
    31801 gtcgtatttt ctcgcgcttt caggaaaatc cttaaaaatg tgaccgattg gtcagacgct
    31861 gcagaagcag taagatagaa gatgagtgtc taattccctg aatacaggag taaataatgc
    31921 aaaaaatcgt gatcgttgcc aatggcgcac cttacgggag cgaatccttg tttaacagct
    31981 tgcggctggc cattgcgtta cgagagcagg agaacaatct ggatctgcgt ctgttcctga
    32041 tgtctgatgc ggtcacagcc gggttgcgcg ggcaaaaacc aggggaaggc tacaacattc
    32101 agcaaatgct ggagatcctt accgctcaga atgtaccggt gaaattgtgc aaaacctgta
    32161 ccgacgggcg cgggattagt acacttcctc tgattgatgg ggtggaaatc ggtactcttg
    32221 ttgaactggc gcaatggacg ctgtcagccg ataaagtgct cacattttaa tgtttgaggg
    32281 ggcgtcggac gccccactat gagggaagtt tactcaggcg aatcccggcc gtaacacacc
    32341 atccggataa ttctccgcca gcagtttttt taccgatacc agcaaatcat tcagcccatc
    32401 gtcctgcatt cccagtttga taagctcctg ttccagtgaa aacaggtatt gtgcattggt
    32461 tcccagcgga ccgctcgccg cggcaatcaa cggcgctatg acctgagcgc gagtgtcaga
    32521 ttcatattct ggatgtcgcg ggtccataat aaacacaatg gcgtttactg tgcgtccatc
    32581 atcaagatca agctgacacc aggttggcag ataacagccg gtaatcatct cacgcttcca
    32641 cagcagggtt agttcctgct ccagcgtctc ttctggcagt cgataggcga cgcctgtggt
    32701 gcgtccgccc tctttcagtg caagcattcg tcccggctgg tgcgcagtcc cgcgcccggc
    32761 ggtcagacgc aggcaaaacg cgcgatgcca tccaaccagt gtaccggtgc acgactcggt
    32821 aaactccagt gccggattcc acatcaacga tccataaccg aagatccaca ccggtccctc
    32881 atcaggtcga caagccagcg tcgccgccag cgaagccgcc cgttgttctg ctgaccataa
    32941 gagtgattct tcaatggcac caaatgccgt tttacaatcg gcattcatca agaaatcacg
    33001 cgttatcacc ttacacctcc gccactgcat acttccctgc gacaactttt tggtgtcttc
    33061 cggacatttt tactgctcat tttgaaacca ctaacgggac aatatgcaat ccacggacca
    33121 cttgcaaggc aacaccgcac gaatagcttt aactaagcta ccggtttgac gattttttat
    33181 gccatttatc atcatcccct ttggcatatt catgcttcac agcagcccag gccactttat
    33241 gcgctgtttc ttcgcgactg gcgtcatcac gccgatcttc tttatcttta tattgatccc
    33301 atgcgctgtt gaacgcttct ttatagatat cctgggcatg agacggaagc acgtgcttta
    33361 cgctttccgg cagatcgctt ttcgttttat acgacatcgt tgactccttt tacaaagttg
    33421 aacattaagt gtggtcaaca atatctactc ttgccaaaac ataagaattt tcatgtcaga
    33481 ctcttcttaa gaagtgctaa aacaaacatt gtctaaacgt attacttatt tttatgccca
    33541 aatgagtaaa aaaccgacag cggggtaaaa attcgtaaaa tgttaccgat atccagacaa
    33601 tttctgttat ttttcctacc tattttttac attttcgata aattcatctg caaagggata
    33661 accataatgt caaatgctca agaggcggta aaaacccgcc acaaggagac ttcgcttatt
    33721 ttcccggttc tggcgctggt agtgctgttc ctgtggggaa gcagccagac actaccagtg
    33781 gtcattgcca tcaatcttct tgcgcttatt ggtattttaa gtagcgcctt tagtgttgtc
    33841 cgtcatgcgg acgtattagc ccatcgcctg ggagaacctt acggttcgct tattcttagc
    33901 ctttcagtgg ttattcttga agtcagtttg atttcagctt taatggcaac cggcgatgcc
    33961 gcgccaacgc taatgcgtga tacgctctat tcaatcatta tgattgttac aggtgggctg
    34021 gttggctttt cattattgtt gggcggacgt aagtttgcca cccaatatat gaatctgttt
    34081 ggtatcaagc agtatttaat tgcgctgttc cccctggcga taatcgtact ggtatttcca
    34141 atggctctgc ctgcggcgaa tttttcaaca ggtcaggcgt tactggtagc attaatttct
    34201 gcggcaatgt atggcgtatt tttgctgatc cagaccaaaa cgcatcaaag tttgtttgtc
    34261 tacgagcacg aagatgacag tgatgatgac gacccgcacc acggtaaacc gtctgcccat
    34321 agcagcctgt ggcatgccat ctggttgatt gtccatctga ttgccgttat tgcagtcacc
    34381 aaaatgaacg ccagcccgct ggagacattg ctcgacagca tgaatgcgcc tgtcgccttt
    34441 actggcttcc tggtggctct gttgattctg tcgccggaag gtttaggtgc gttaaaagca
    34501 gtgttgaata accaggtcca gcgcgcgatg aatctgttct ttggttcagt gttggcaacc
    34561 atttcgctta ccgtacctgt cgtcacgcta attgccttta tgacgggtaa cgaattgcag
    34621 tttgcacttg gtgcgccaga aatggtggtg atggtggcct ctttagtact gtgccatatc
    34681 tccttctcca ccggacgaac taacgtgctc aatggcgcag cgcatctggc actgtttgcc
    34741 gcctatttga tgacgatatt tgcctgacag ttatatctcc tccgatgacg gaaatcgggg
    34801 gagataatat aaattcctgc ttattatatt tcttcatact agctggacag gttgttaatc
    34861 agaattgcaa gcctgtacca caacctgcac gaaatgactg caacagctgt tgaaaaaccg
    34921 gaatgtactc tttattgcaa acatttcagg gatgaagtga agatgaattg cgacgtagta
    34981 tccacaccaa gccggcgtta tccggtgagg cgcaatgttg cgggggcttt atccctggtg
    35041 gcattggttg ctggaaagag aaaacccccg cacgttgaga ggtacaacct gacaacagaa
    35101 cgggggctaa tcttgaacca gaacaattca agaatagccg caaaatgttg tcattacaac
    35161 aaggcggcta tatgacgctc gcgcagtttg ccatgatttt ctggcacaac ctggcggcac
    35221 cgatcctggc ggggattatt accgcagtga ttgtcagctg gtggcgtaac cggaagtaag
    35281 gtgaaaacgg gcgtcaggct gccgtgatgg caatatgcgc ccgaaaccgg ccgcaggggt
    35341 aacagcctgc ggcctttttt gtatccggca cccatgaaaa aagtcttaac gcagaacgct
    35401 aagactttat ttttcaggca aaaaagatta cttgctggta tccagttctt cgaaaccttt
    35461 caccagatca tcaatcgctt tcatttgctt gaggaacggt tccagtttag ccagcggcag
    35521 cgcggatgga ccatcacatt tcgcatgttc cggatccgga tgcgcttcaa taaacagccc
    35581 cgccagacct accgccatac cggctcttgc cagctcagcc acctgagcac ggcgaccgcc
    35641 ggaagctgcg ccaaatggat cgcgacattg cagtgcgtgg gtaacgtcga aaatcaccgg
    35701 cgaattacca gacactttct tcatgatgct gaagcccagc atatcgacaa ccaggttgtc
    35761 atagccgaag ttagcaccgc gatcgcaaag aatcactttt tcgttgccgc cttctttgaa
    35821 tttatcaacg atattaccca tctgtcccgg gctgacaaac tgtggtttct tgacgttaat
    35881 taccgcaccg gttttcgcca tggcttcaac caggtcagtc tggcgagcaa gaaacgccgg
    35941 caactgaatc acatccacga catcagcaac gggctgtgcc tgacttggtt cgtgaacgtc
    36001 ggtgataatt ttcacgccaa aagtctgctt caactcctgg aagattttca tcccttcttc
    36061 caggcccggt ccacgataag agtggatgga ggagcggttg gctttgtcaa aagaggcttt
    36121 gaacacgtaa gggataccca gtttctgggt cacggttacg tagtgctcgc aaatgcgcat
    36181 cgccagatcg cgagattcca gcacgttcat accgccaaac agtacgaacg gcaggtcatt
    36241 tgctacgttg atgtcaccaa tgctaaccac tttttgtttc ataggatcgc cttaatcttg
    36301 agtaaaatgt cgattaatta atgcagcaca atatgtttat gcgcgatgtt atttatttgc
    36361 gcacggatca tttcgctgat cgggtcttcc ggacactgtt caacgaaata acttaaatcg
    36421 ttcaacgcaa cgtgttcgca atccagttgc gcataaatca acccgcgatc gcgaatttca
    36481 tagggatctt ccgggttgaa ttgtaataaa gcttcgctgg tgcgtaacgc cagctccatc
    36541 tgattttctt ccatcaacga agctttgagt gtatccagca atttgcggat tacctcaatg
    36601 ttatcagctt catcaaggtc ttcataaaac agttccgccg acgggctgat atttcccttt
    36661 aaccacacat ccagcatatg ttcgcttaac gattcaccgt taaaaggatt aatcagccaa
    36721 atttcgccat ccggacattc aatgcgcaat atcagctgcg tagggaaaat caccggcagc
    36781 agcggcaaat cgagacgatt cgcgacccat aataaaaccg cacctaatga taccgcactg
    36841 ccctgtcgat tctttaacac ctggtccagc cataatgcat cggaaagacg ataaacgccg
    36901 cgtgaggctt taaatcccca gtcgccgtaa aacagcgcga tcagtttttc caactgctct
    36961 tctaaaggca gaagctggct aatttcttcc ttcgccagac taacgagacg ctccagttcg
    37021 tcgtaaacat cttgcgaggg aaaatcgcgg cggattgctt cgcaagccag gatcatgcct
    37081 tcgcacaatg gcgctttatt aaattcgaaa tcagctaacg atctcatgac ttaccccagt
    37141 aacggtactt ttgtggtggc gagtttaatg atgatgtaca gcaccaccaa cgccagcggg
    37201 aaggcaataa tgcgcgcctg ctgactgtgc atccgacgat aatcgagtgc aataaaaccc
    37261 aaaacgatat aaatgataac tccaaacagc ttttcagtca gccactgtgc ctgcccggaa
    37321 aatggctgga tgtgcgcttt agccatcaac gcaatgccgc ttaacaataa cagcgtatcg
    37381 acaaccggcg gcacaatgcg cgtccagcga gcaaatgcct gtggatgctt ctgataacgt
    37441 agccagaaac gtaaggtcaa tagcccaaca gaaagcgcga tactaatgag atgaacgcta
    37501 agcaacgtag aaaaacttgt cattgataat agcggccgag cgttacgcgc tcgttatccc
    37561 catagtcacg gcaggtttcg acgtcatgat accccgcgag gataaatgcc tgtcgcaccg
    37621 cttcaccctg ctgccagcca tgctccagaa gcagaaagcc gccggatacc agcgcgttac
    37681 gcgactgttc gatgatatgc acgatgtctg ccattccact gtctgccgca accagcgcag
    37741 tgagcggctc aaagcggaca tcgccttgtt gaagatgtgg gtcctgctcg tcaatatacg
    37801 gcggattgct gacaatcatc gcaaactgct gcccggctag cgcgctaaac cagtcgcttt
    37861 gcagaatgtg gatatttttg atagccagat gctgggcatt acgttgtgcc agggcgacag
    37921 catcgggcat acgatctaca gcgataattt cgcagtccgg gcgctcgctg gcaagcgcaa
    37981 gcgcaatcgc cccggtaccc gttccgagat cgagaatacg gcaaggttgt tcaggcaacc
    38041 gcgccagtgc ctgctccacc agacactccg tatccgggcg cggaattaag gtcgctggcg
    38101 aaacaaataa cggcaacgac cagaattctc gcaccccggt taaatgagca atgggttcac
    38161 catcgcgacg acgcgtcagt agcgcatcaa gttgctgaca ttgttcgtca gtcagctgcg
    38221 tttcaccaaa ggcgagaata aaagtacgcc ctttgccggt aacatgctcc agcaggattt
    38281 cagcatcacg ccgcgggctt tcgctcgcct gaagttggct tattgcttca cgtaaccagt
    38341 gttgatattc cattattcct gctcggacaa cgccgccagt tggtcggcct gatgttcctg
    38401 gataatcggt tcaatcagca tatccagctt accttccatc acttcatcca ggcggtagag
    38461 cgtcaggttg atgcggtgat cggtaacgcg cccctgcggg aagttgtaag tacggttacg
    38521 gtcgctgcga tcgccactcc ccagcaggtt acgacgggta gacgcttcgg cctgttgccg
    38581 ttttgccatt tcagcagcgt ggatgcgagc accgagtaca gaaagtgctt tagctttgtt
    38641 tttatgttgt gaacgttcgt cctgacattc aacaacaatc ccggtcggca agtgagtaat
    38701 acgaattgcc gaatcggtgg tgttaacgtg ctgaccaccc gcccctgacg agcggaaagt
    38761 atctatgcgc aaatctgctg ggttgatgtc cggcagttct gcatcaggca gttctggcat
    38821 taccgcaacg gtacaagcag aagtatgaat acgaccctgc gattccgtag caggaacacg
    38881 ctgcacgcga tgaccgccag attcgaattt caaacgacca tatacaccat caccgctaat
    38941 tttggcgatg atctctttat aaccaccatg ttcaccctcg ctggcgctca tgatttctac
    39001 acgccagcgg cgggcttcgg cgtaacggct gtacatacgg aacagatcgc cagcgaacag
    39061 cgccgcttcg tcgccgccgg ttccggcacg gacttcgagg aaggcgttac gttcgtcatc
    39121 aggatctttt ggtagtaaca gaacctgtaa ttgctgttcc agttgctcgc ttttttcttt
    39181 agcttcgcgc agttcatcct gcgccatctc acgcatttca ggatcgtcaa gcatcatctg
    39241 tgcggtttcg atatcttcct gaacctgttg ccagtcggta aaacagcgcg aaacatcact
    39301 taactgcgca tattcgcgtg ataatgcgcg aaaacgttcc tggtcggcga tagtttgcgc
    39361 gtcacccagc aacgcctgaa cttcttcatg gcgttcatgc agggcttcca gtttggcaac
    39421 gatagaaggc ttcataggcg taaatgcacc ctgtaaaaaa agaaaatgat gtactgctac
    39481 tccagcccga ggctgtcgcg cagaatattc aggcgttcgt tatccccgtc acgggcggcc
    39541 tgttgaagtg attttgttgg cgcatggatc aagcggttag tcagtttcca tgccagatcc
    39601 tgcataatgg cttgtgcgtc gccgccctgc tcaagggccg ctaacgcttt ggcggttaac
    39661 tcatcgcgaa cgtgctctgc ctggctgcga tactcgcgaa tagtttcgct ggcgctttgt
    39721 gcacgcagcc acgccataaa ttcgctggtt tcctgagcga caatagtttc cgcctcaacc
    39781 gctgcggctt tacgctgcgc caggttgtgc gaaatgatgc tttgcagatc gtccacgcta
    39841 taaagataag cattcgccag tttgccaact tccggctcaa catcgcgcgg aacagcaata
    39901 tccaccaaca gcattggttg gttgcgacgg ctttttaatg cgcgctccac catgcctttc
    39961 ccgataatcg gtaacgggct ggcggtggaa ctgatgatga tatcggcttc gcgcagacgt
    40021 tcgtcgatat cactcagggc aataatttcc gcgccgactt catctgccag aatttgggca
    40081 cgttcgcgag tgcggttggc gataatcatc ttctgtactt tgtgttcgcg cagatgacgc
    40141 gccaccagct cgatggtttc gcccgcaccc accagcagta ctgtcaccgt agagagcgat
    40201 tcaaagatct gccgcgccag cgtacaagcc gcaaaagcga cagacacagc actggcaccg
    40261 atatctgttt cagtgcgaac gcgtttcgcc acagagaaag atttctgaaa catgcgttcc
    40321 agttcgctgg ccttcatatg acctttttgc gaatcggcaa acgctttttt aacctgaccg
    40381 aggatctgcg gctcgccaag gacgagagaa tcaagcccac tggcaacgcg cattaaatga
    40441 ctgaccgcat cgttatcctg atgccagtag aggctattac gcagatcgtc ttcgttgaga
    40501 ttatggtaat cacacaacca gcgaatgagc gcctcttgca ggtcatcccg ttcttcaacg
    40561 ctgagataaa gttctgtgcg gttacacgtc gacagcacca cgccgccctg caccatcggc
    40621 tgcgcgagca gactgtcaag cgcctgatcg agcttatccg gcgaaaacga tacacgttct
    40681 cgcagcgata caggtgccgt tttatggttg ataccgagtg ctaaaagggt catgtctgcg
    40741 ggaaataata ccaacgttga tagggttagt ctgcttgcat catacaggat gcgtaagatc
    40801 aataaaagag agcgccccct tttggagtaa ttgccgaaag ccgttagatt ctggcaatta
    40861 agacaacgtg aacatagacg atggctaacg gtaacgctag cattaagggt tataactgca
    40921 acgtatctca aggacttgtc attattatga ccctgcccga ttttcgcttt atccgcctgc
    40981 taccgctagc tgccctcgtg ctcactgcct gttctgttac cacacccaaa ggcccaggca
    41041 aaagcccgga ttcgccacag tggcgtcagc accagcaaga cgtgcgtaat cttaatcagt
    41101 atcagactcg cggtgcattc gcttatattt ccgaccaaca aaaagtgtac gctcgcttct
    41161 tctggcaaca aaccggccag gatcgctatc gtctgctgtt aactaaccca ttgggcagca
    41221 cggaactgga gctgaatgct cagccgggta acgtacaatt agtcgacaat aaaggtcagc
    41281 gttacaccgc cgatgacgcc gaagagatga ttggtaaact aaccggaatg ccgattccgc
    41341 tcaacagctt gcgccagtgg attttaggtt taccgggtga tgcgaccgat tacaaactgg
    41401 acgaccagta ccgcctgagc gaaatcacct acagccagaa tggcaaaaac tggaaagttg
    41461 tttacggtgg ttatgacaca aaaactcaac ctgcgatgcc agccaatatg gaactcaccg
    41521 acggtggtca acgcatcaag ttaaaaatgg ataactggat agtgaaataa tgcggacaca
    41581 gtggccctct ccggcaaaac ttaatctgtt tttatacatt accggtcagc gtgcggatgg
    41641 ttaccacacg ctgcaaacgc tgtttcagtt tcttgattac ggcgacatca tcagcattga
    41701 gcttcgtgac gatggggata ttcgtctgtt aacgcccgtt gaaggcgtgg aacatgaaga
    41761 taacctgatc gttcgcgcag cgcggttgtt gatgaaaact gcggcagaca gcgggcgtct
    41821 ttcgacggga agcggtgcga atatcagcat tgacaagcgt ttgccgatgg gcggcggtct
    41881 cggcggtggt tcatccaatg ccgcgacggt tctggtggcg ttaaatcatc tctggcaatg
    41941 cggactaagc atggatgagc tggcggaaat ggggctgacg ctgggcgcag atgttcctgt
    42001 ctttgttcgt ggtcatgccg cgtttgccga aggcgttggt gaaatactaa cgccggtgga
    42061 tccgccagag aagtggtatc tggtggcgca ccctggtgta agtattccga caccggtgat
    42121 ttttaaagat cctgaactcc cgcgcaatac gccaaaaagg tcaatagaaa cgttgctaaa
    42181 atgtgaattc agcaatgatt gcgaggttat cgcaagaaaa cgttttcgcg aggttgatgc
    42241 ggtgctttcc tggctgttag aatacgcccc gtcgcgcctg actgggacag gggcctgtgt
    42301 ctttgctgaa tttgatacag agtctgaagc ccgccaggtg ctagagcaag ccccggaatg
    42361 gctcaatggc tttgtggcga aaggcgttaa tctttcccca ttgcacagag ccatgcttta
    42421 agccgggcaa gctgagtttc ggtgacaacg tcaccttgtt ccagacgttg catcgcgctc
    42481 tttaatacac cgcctggaaa gaatcatgcc tggcccgcac agttttcggc agattctttc
    42541 caccaatgga cgcatgcctg aggttcttct cgtgcctgat atgaagcttt ttgctggtaa
    42601 cgccaccccg gaactagcac aacgtattgc caaccgcctg tacacttcac tcggcgacgc
    42661 cgctgtaggt cgctttagcg acggcgaagt cagtgtacaa attaatgaaa atgtacgcgg
    42721 tggtgatatt ttcatcatcc agtccacttg tgcccctact aacgacaacc tgatggaatt
    42781 agtcgttatg gttgatgccc tgcgtcgtgc ttccgcaggt cgtatcaccg ctgttatccc
    42841 ctactttggc tatgcgcgcc aggaccgtcg cgtccgttcc gctcgtgtac caatcactgc
    42901 gaaagtggtt gcagacttcc tctccagcgt cggtgttgac cgtgtgctga cagtggatct
    42961 gcacgctgaa cagattcagg gtttcttcga cgttccggtt gataacgtat ttggtagccc
    43021 gatcctgctg gaagacatgc tgcagctgaa tctggataac ccaattgtgg tttctccgga
    43081 catcggcggc gttgtgcgtg cccgcgctat cgctaagctg ctgaacgata ccgatatggc
    43141 aatcatcgac aaacgtcgtc cgcgtgcgaa cgtttcccag gtgatgcata tcatcggtga
    43201 cgttgcaggt cgtgactgcg tactggtcga tgatatgatc gacactggcg gtacgctgtg
    43261 taaagctgct gaagctctga aagaacgtgg tgctaaacgt gtatttgcgt acgcgactca
    43321 cccgatcttc tctggcaacg cggcgaacaa cctgcgtaac tctgtaattg atgaagtcgt
    43381 tgtctgcgat accattccgc tgagcgatga aatcaaatca ctgccgaacg tgcgtactct
    43441 gaccctgtca ggtatgctgg ccgaagcgat tcgtcgtatc agcaacgaag aatcgatatc
    43501 tgccatgttc gaacactaat cgaacccggc tcaaagaccc gctgcggcgg gtttttttgt
    43561 ctgtaatatc catttgtatg acctatgcct ccttcacctg ccatttagtt gacagatgat
    43621 gcgctcacgg atgaaacatt attgtgaaca aaatattttc ctcacatgtg atgcctttcc
    43681 gcgctctgat cgacgcttgc tggaaagaaa aatatactgc cgcacggttt acccgtgacc
    43741 tgattgccgg gataaccgtc gggattattg ctatcccgct ggcgatggcg ttggctattg
    43801 gtagtggtgt ggcaccccag tacggtttat ataccgcagc tgttgcgggg attgtcattg
    43861 ctctgacggg agggtcacgc tttagcgttt ccggtccgac tgcggcattt gtggtaattc
    43921 tctatcccgt ttcgcaacag tttggactgg caggactgct ggttgcgacc ttgctgtcgg
    43981 ggatcttttt gattctgatg gggctggcac gctttggtcg cctgattgag tatattccgg
    44041 tttccgtcac cttaggtttc acctcgggta tcgggatcac catcggtacc atgcagatta
    44101 aagattttct cggtctgcaa atggcccatg tcccggaaca ttatctacaa aaagtcggcg
    44161 cattatttat ggcgctgccg accattaatg tgggtgatgc agccattggc attgtgacgc
    44221 ttggcattct ggttttctgg ccgcgtctgg gcatacgttt acccggccac cttccggcct
    44281 tgctggctgg ttgcgcggtg atggggattg ttaacctgct cggcggacat gttgctacca
    44341 tcggttcgca attccactac gtcctggccg atggttctca gggtaacggt attccgcaac
    44401 tgctgccgca actggtgctg ccgtgggatc tgcctaattc agaattcacg ctaacctggg
    44461 attctattcg cacactgctg cctgcggcat tctcaatggc aatgctcggc gcaatcgaat
    44521 ctctgctctg cgccgtggtg ctggatggta tgaccgggac gaaacacaag gcgaacagcg
    44581 aactggttgg acagggactg gggaatatta tcgctccgtt ctttggtggc attaccgcta
    44641 cagctgccat cgcgcgttct gccgctaacg tccgtgccgg ggcaacttcc cctatctcgg
    44701 cggtgatcca ctctattctg gttattcttg ccctgctggt actggcaccg ctgctctcct
    44761 ggctgccact ttccgccatg gcagccctgc tgttgatggt ggcgtggaac atgagtgaag
    44821 cgcacaaagt ggtcgacttg ctgcgtcatg caccgaaaga tgacatcatc gtcatgctgc
    44881 tgtgcatgtc gctgaccgtg ctgttcgata tggttattgc catcagcgtg gggatcgtgc
    44941 tggcatcgct gctgtttatg cgtcgtatcg cacgtatgac tcgcctggca ccggtagtcg
    45001 tagacgttcc agacgatgtc ctggttctgc gcgttattgg cccgctgttt tttgctgccg
    45061 ctgaaggctt attcacggac ctggagtcac gtcttgaagg caaacggatt gtgattctga
    45121 agtgggatgc cgttccggta cttgatgctg gtggtcttga tgcgttccag cgttttgtga
    45181 agcgtctgcc cgaaggatgt gaactgcgcg tgtgcaacct ggaattccag ccactgcgca
    45241 ctatggctcg cgcaggcatt caaccgatcc cgggacgcct cgcattcttc ccgaatcgtc
    45301 gcgcggcgat ggcggattta taagtacgag attgaccagt cagcgcaaac tgactggtca
    45361 gcaaactgca ttatttgtta gctatgacgg cgattatcgc tacggcggca acgtttgtca
    45421 tagtggcgaa cccaccagta acggtcgcac acctgttcat gcccgccaac acgggcaccc
    45481 gccagccaga gaatcgcacc gacgaaaata cttagcacag caccatgtgc gaaatactgg
    45541 ggcatattaa actgtggtaa ctggttgagg attgaatacc ccacgccgac caccattacc
    45601 accagaccca accccatgag cacgttaccg agtaacgaag cgtttttgcg tttcatatgt
    45661 cacctccgga actttctggg ttgtaacagg gaatacccct cttccttatg tgtaaagtat
    45721 agacaacacc ctgtgggtta tgtgcgggtg tgatcacaat tacaaccctt atttcaacaa
    45781 aactttacaa ataaacgcct gaacaccttg actttctaat agtccactta ggcaataatt
    45841 gcattatctg catttgatac gcagtttttt gtcaagcggg gccgcaacca gtaaactacg
    45901 cgccagttat gtacacactc aggacaaaaa aacgtgacga ttaaattgat tgtcggcctg
    45961 gcgaaccccg gtgctgaata cgccgcaacg cgacataatg ctggtgcctg gttcgttgac
    46021 ttactggcag agcgattgcg cgctccgctg cgcgaagagg ctaaattctt tggttatact
    46081 tcgcgagtca cccttggagg cgaagatgtc cgcctgttag tcccgactac atttatgaat
    46141 ctcagcggca aagccgttgc ggcgatggcc agttttttcc gcattaatcc ggacgaaatt
    46201 ctggtggccc acgacgaact ggatctgcct cctggcgtcg ccaaatttaa gttgggtagc
    46261 ggtcatggtg gtcacaatgg actgaaagac atcatcagta aattgggtaa taaccctaac
    46321 tttcaccgtt tacgcatcgg aatcggtcat ccgggcgata aaaataaagt tgtcggtttt
    46381 gtgttaggca aaccgcctgt tagtgaacag aagttaattg atgaagccat cgacgaagcg
    46441 gcgcgttgta ctgaaatgtg gtttacagat ggcttgacca aagcaacgaa ccgattgcac
    46501 gcctttaaag cgcaataagt cgttgtctgc ggcatttttg ccgagtgccg tgtataatag
    46561 gcaaagttat ttccatttct gcaatctgtt agcaataaca ggttgattat taagatatta
    46621 aggtgattta aatcatggga ttcaaatgcg gtatcgtcgg tttgcccaac gtcgggaaat
    46681 ctaccctgtt caacgcgctg accaaagccg gtattgaagc ggccaacttt ccattctgca
    46741 ccattgagcc gaacactggc gtcgtaccaa tgcctgaccc tcgcctggat caactggctg
    46801 aaatcgtaaa accgcagcgt acacttccca cgaccatgga atttgtcgat atcgccggtc
    46861 tggtaaaagg cgcatcgaaa ggcgaaggtc tgggtaacca gttcctgacc aacatccgtg
    46921 aaaccgaagc gatcggtcac gttgttcgct gctttgaaaa tgacaacatc attcacgttt
    46981 ccggcaaagt taacccggcg gatgatattg aagttatcaa caccgaactg gcgctggcgg
    47041 atctcgacac ctgtgaacgt gcgattcatc gcgtacagaa gaaagccaaa ggtggcgata
    47101 aagacgcgaa agccgagctg gcggtactgg aaaaatgcct gccccagctg gaaaacgcag
    47161 gtatgctgcg cgcgctggat ttaagcgctg aagagaaagc ggctgttcgt tacctgagct
    47221 tcctgacgct aaaaccaaca atgtacatcg ccaacgtcaa cgaagacggt tttgaaaaca
    47281 acccgtatct tgaccaggtg cgtgaaatcg cagcgaaaga aggttctgtt gtagttccgg
    47341 tttgtgctgc tgttgaagca gacattgccg aactggacga cgaagagcgt gacgagttta
    47401 tgcaggagct tgggctggaa gagccgggcc tgaaccgtgt gatccgtgcc ggttataagc
    47461 tgctgaacct gcaaacttac ttcaccgctg gggtgaaaga agtgcgtgca tggaccattc
    47521 cggttggtgc aaccgcgccg caggcagcgg gcaaaatcca tactgatttt gaaaaaggct
    47581 ttatccgtgc acaaaccatc tcgtttgaag atttcatcac ttacaaaggt gaacaaggcg
    47641 cgaaagaagc aggcaaaatg cgtgcagaag gcaaagatta catcgttaaa gatggcgatg
    47701 tgatgaactt cctgttcaac gtctaaatta aatctgctgt ctcatgaggt ttcactgaat
    47761 ctcataagaa ccgtaaaact ctataaaatc cacgcaattg cgtggatttt tcatttcgca
    47821 atgtctcaat tcatctcata acaacgctgt caaaaatgag tacatggatg agtacaacat
    47881 tcttccatct tcattttaag tgagtacaat aacggtataa taaataaaca aggccccgtt
    47941 atgctcactg atacgcaatg ccgcactgct aagccgaaag aaaaactcta tcgacttaat
    48001 gatttcaatg gtctctacct cgaagtgaaa cccaatggta aaaaggcatg gcgctatcgg
    48061 tttaaactca acggtaaatc cagcatgttt gcgctgggtg agtacccgac ggtaaaactt
    48121 gccgaagctc gcgagaaatg cgagcaagcg cgtaagcaag tcgcagatgg agttagccca
    48181 acacaggctc gtcagttaga taagatccgc aaggtcaacg acgcatccaa cacctttgag
    48241 ctaatcgcta aagaatggtt gcagatgaaa gactgggccg gaattaccaa aacgcggcga
    48301 ctggatatgc ttgaacgtgt ggtttttccc gccatcggta agctccctgt cagagagatc
    48361 actccacacc atatacttaa aattcttcag gaaaccgcta aacgaggagc accaacagtt
    48421 gccgctgaag cccgtcgcac catttcatca gtttttgagt tggcagtcgc gacgcttaga
    48481 gctgatagcg atcccgtatg gcctgtgcgc aaggcccttc cggctaataa gacacagcac
    48541 aaacaggcac taaatcccca acaaatcgga aagttactaa gctgttttga caatagtcgt
    48601 ggctcgtatc aggttaacta ttgcatgtgg cttatgtggt ggacattggc gcgtcctgca
    48661 gaagccaccg aagcagaatg gacagaattc aatcttgata acgccctatg gactattccg
    48721 gcagcgcgaa tgaaagcccg cagagaacac gttattcccc tacccttcca agctgtcaaa
    48781 atgctcagaa cactacaggg attaaccggg catcggcagc accttttccc gggcagagac
    48841 aatccgctag gtccaatgac atcgcactca ctgcgccagc tacttaaaag cctcggctgg
    48901 agtggtacat atagcccaca tgcaacccgg accacaggaa gtacccgttt aaacgagttg
    48961 ggctatcgac ctgatgctat tgaggcccaa cttgcgcacg ctgataccaa caatgttcgt
    49021 cgcacataca accatgcaac ttatcttgat gagcgtaaag tgatgatgca ggaatgggct
    49081 gatatgttgg acaaatgggt agcaggattc aagtagaact tgctgcacaa agtgaaccac
    49141 gaggatagaa cttattcctc aaggttctgg agtgccccca agcccgtaga caaatctgcc
    49201 ctatagtttg agcataggag gagtctatgg gcacaccacg atttacccca gagtttaagg
    49261 aagaagctgt ccgtcaggtc actgaacggg gctattccgt tgccgatgtt tctgaacgcc
    49321 tcggcgtatc ggctcacagc ctttataagt gggtccggtc agtcagaccg gataacagcg
    49381 ggcatcaggc gcaggattta ctggatgcgc ggacggagat tctcaggctt aaagcgcagc
    49441 ttaaacgtac tgaggaagaa cgggatattc tgaaaaaggc agcgcggtac tttgcaaggg
    49501 agcccgactg aagtaccgct tcatcaacga tcaccgtgaa atctggtcga tcgtaacaat
    49561 gtgtcgggtt ctgaaggtcg cccgtgcggg attttacgtc tggcttcatc gccctgtttc
    49621 tgccggagaa aaggataatc agcggttgct ggagctcatc cgcgatgctt atgcactgag
    49681 cggcggagtc tacggttatc gcagggttca cggcgatctt cgtgaaatcg gtgaagtatg
    49741 cagcagaaac cgtgttgcca gaatcatgaa gcggaacagg atacaggccg tacatggcta
    49801 taaggtcccg cgtggcgtca ggggaaggcc gtcactgata gctcctaatc gcgtacagag
    49861 ggaatttacg atggtaaaac ccaaccaggt gtgggtgact gatattacct acatccgcac
    49921 ctggcagggt tggctgtatc tggcggtggt tatcgatctt tttgcccgta atgtggtggg
    49981 ctggtcgatg aaaccaacgc tgtcgcgcga gctggcactg gatgcgctgc tgatggcagt
    50041 ctggcggcgt aagcctgcag aaaacgtcat agtgcacagc gatcagggca gtcagtatgg
    50101 cagtgatgac tggcagcggt tctgcagggc caacaatctg gcgccgagta tgagccgtcg
    50161 tggcaactgc tgggataatg cggtggcaga atcgttcttc agttcactga aaaaagagcg
    50221 catcagaaag cggatatata aaacccggga catggcccgg gcggatatct tcgattacat
    50281 tgaagtgttc tacaaccgga cccggcgcca cagtcatctg ggtggcgtca gcccggaggc
    50341 cttcgaaaag gcctcaccgt gaggacagaa actgtctacc ggaatgtggt cactccactt
    50401 gagtcgtctt gctgtactcc tttggccaat ccatttagct ctccaacgtg atgttttcaa
    50461 cggtaacaaa agtaacaata taacatgcag tcatttcaac gatctcagtg ttaccaccca
    50521 ttttgagcaa tcatatcaaa gtaacaaaag gggaatattg tgtggttcat cgctgaaagc
    50581 aggctaagat cacctgccgg ctatgcatat catgaaagtg ctaaatgtcc cgtcttgttt
    50641 atcaaccgag ataagctgta attgactgag tggctcccct taatgctttt tctcaatcaa
    50701 cagatatgga tgggaccagt cagtgacctc tttatatgaa aaaaacccat cgtgctccag
    50761 tgcgcaaacg cccttatgac tggcaacgta atcgtaaaca gcccggcttt ccagtccctg
    50821 taacatgtgc gaaagcagct ttcggcgcat cacatctgac ttcctgcgtt tactgtcaac
    50881 aacgggtaat aaagtgatcc actaaccgtc accaccaacg gtttaatatt gatccacctt
    50941 gtttactcag gattagcttc aggtataacc ccggcctttc gtttctactt cagtcgatag
    51001 ctctctcctt ttatctggac tacgtgtgag tgatgtaaga tacggtccag catcgccgat
    51061 gtcagggctg catccctggc gaacgtctga tcccactgcc cgaacggcag gttggagtcc
    51121 aggatcatcg cgctcctctc gtaacgttta gcgatgacct ggaagaacag cttggcttct
    51181 tccagactga acggcagata acctatttca tcgatgataa gtagcttcgg ggccatgaca
    51241 ccgcgattga gtgtcgtttt gtaacggccc tggcgctatg aagtggatag ctgtagcagc
    51301 aggtccgctg ttgtgaagcg aaccttgatg cctgcccgta gcggcggacg cccatggcac
    51361 aggtgccgca ggcgagcatg cgttcaacgg agagtcgggt ccattagctg atgctgtcag
    51421 cgtatttttc gaggaattta tgtaagaagc gtcaaaatga aagcgccaac taataatgac
    51481 agaattaata aaataccagt aagcgcgtat gctatacctt ttccaatctc gttttttatg
    51541 gtacgtccaa ttgaataaag gattaaagat accgatagca
//
LOCUS       DAHHTG010000016        50203 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.016, whole genome shotgun sequence.
ACCESSION   DAHHTG010000016 DAHHTG010000000
VERSION     DAHHTG010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 50203)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 50203)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..50203
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.016"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            194..1141
                     /gene="cyoA"
                     /locus_tag="NBI74_000947"
     CDS             194..1141
                     /gene="cyoA"
                     /locus_tag="NBI74_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit II"
                     /protein_id="HCD1931316.1"
                     /translation="MRLRKYNKSLGWLSLFAGTVLLSGCNSALLDPKGQIGLEQRSLI
                     LTAFGLMLIVVIPAILMAVGFAWKYRASNKDAKYSPNWSHSNKVEAVVWTVPILIIIF
                     LAVLTWKTTHALEPSKPLAHDEKPITIEVVSMDWKWFFIYPEQGIATVNEIAFPANTP
                     VYFKVTSNSVMNSFFIPRLGSQIYAMAGMQTRLHLIANEPGTYDGISASYSGPGFSGM
                     KFKAIATPDRAAFDQWVAKAKQSPNSMSDMAAFEKLAAPSEYNQVEYFSNVKPDLFAD
                     VINKFMAHGKSMDMTQPEGEHSAHEGMEGMDMSHAESAH"
     gene            1163..3154
                     /gene="cyoB"
                     /locus_tag="NBI74_000948"
     CDS             1163..3154
                     /gene="cyoB"
                     /locus_tag="NBI74_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002444673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit I"
                     /protein_id="HCD1931317.1"
                     /translation="MFGKLSLDAVPFHEPIVMVTIAGIILGGLALVGLITYFGKWTYL
                     WKEWLTSVDHKRLGIMYIIVAIVMLLRGFADAIMMRSQQALASAGEAGFLPPHHYDQI
                     FTAHGVIMIFFVAMPFVIGLMNLVVPLQIGARDVAFPFLNNLSFWFTVVGVILVNVSL
                     GVGEFAQTGWLAYPPLSGIEYSPGVGVDYWIWSLQLSGIGTTLTGINFFVTILKMRAP
                     GMTMFKMPVFTWASLCANVLIIASFPILTVTVALLTLDRYLGTHFFTNDMGGNMMMYI
                     NLIWAWGHPEVYILILPVFGVFSEIAATFSRKRLFGYTSLVWATVCITVLSFIVWLHH
                     FFTMGAGANVNAFFGITTMIIAIPTGVKIFNWLFTMYQGRIVFHSAMLWTIGFIVTFS
                     VGGMTGVLLAVPGADFVLHNSLFLIAHFHNVIIGGVVFGCFAGMTYWWPKAFGFKLNE
                     TWGKRAFWFWIIGFFVAFMPLYALGFMGMTRRLSQQIDPQFHTMLMIAASGAVLIALG
                     ILCLVIQIYVSIRDRDQNRDLTGDPWGGRTLEWATSSPPPFYNFAVVPHVHERDAFWE
                     MKEKGEAYKKPDHYEEIHMPKNSGAGIVIAAFSTIFGFAMIWHIWWLAIVGFAGMIIT
                     WIVKSFDEDVDYYVPVAEIEKLENQHFDEITKAGLKNGN"
     gene            3144..3758
                     /locus_tag="NBI74_000949"
     CDS             3144..3758
                     /locus_tag="NBI74_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit III"
                     /protein_id="HCD1931318.1"
                     /translation="MATDTLTHATAHAHEHGHHDAGGTKIFGFWIYLMSDCILFSILF
                     ATYAVLVNGTAGGPTGKDIFELPFVLVETFLLLFSSITYGMAAIAMYKNNKSQVISWL
                     ALTWLFGAGFIGMEIYEFHHLIVNGMGPDRSGFLSAFFALVGTHGLHVTSGLIWMAVL
                     MVQIARRGLTSTNRTRIMCLSLFWHFLDVVWICVFTVVYLMGAM"
     gene            3758..4087
                     /locus_tag="NBI74_000950"
     CDS             3758..4087
                     /locus_tag="NBI74_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit IV"
                     /protein_id="HCD1931319.1"
                     /translation="MSHSNASSGASHGSVKTYMTGFILSIILTVIPFWMVMTGAASPA
                     VILGTILAMAVVQILVHLVCFLHMNTKSDEGWNMTAFVFTVLIIAILVVGSIWIMWNL
                     NYNMMMH"
     gene            4099..4989
                     /gene="cyoE"
                     /locus_tag="NBI74_000951"
     CDS             4099..4989
                     /gene="cyoE"
                     /locus_tag="NBI74_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX farnesyltransferase"
                     /protein_id="HCD1931320.1"
                     /translation="MIFKQYLQVTKPGIIFGNLISVIGGFLLASKGSIDYPLFIYTLV
                     GVSLVVASGCVFNNYIDRDIDRKMERTKNRVLVKGLISPAVSLVYATLLGIAGFMLLW
                     FGANPLACWLGVMGFVVYVGVYSLYMKRHSVYGTLIGSLSGAAPPVIGYCAVTGEFDS
                     GAAILLAIFSLWQMPHSYAIAIFRFKDYQAANIPVLPVVKGISVAKNHITLYIIAFAV
                     ATLMLSLGGYAGYKYLVVAAAVSVWWLGMALRGYKVADDRIWARKLFGFSIIAITALS
                     VMMSVDFMVPDSHTLLAAVW"
     gene            5138..6502
                     /locus_tag="NBI74_000952"
     CDS             5138..6502
                     /locus_tag="NBI74_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCD1931321.1"
                     /translation="MNDYKMTPGERRATWGLGTVFSLRMLGMFMVLPVLTTYGMALQG
                     ASEALIGIAIGIYGLTQAVFQIPFGLLSDRIGRKPLIVGGLAVFAAGSVIAALSDSIW
                     GIILGRALQGSGAIAAAVMALLSDLTREQNRTKAMAFIGVSFGITFAIAMVLGPIITH
                     KLGLHVLFWMIAILATTGIALTIWVVPNSSTHVLNRESGMVKGSFSKVLAEPRLLKLN
                     FGIMCLHILLMSTFVALPGQMADAGFPAAEHWKVYLATMLIAFGSVVPFIIYAEVKRK
                     MKQVFVFCVGLIVVAEIVLWNAQTQFWQLVVGVQLFFVAFNLMEALLPSLISKESPAG
                     YKGTAMGVYSTSQFLGVAIGGSLGGWIDGMFDGQGVFLAGAMLAAVWLAVASTMKEPP
                     YVSSLRIEIPANIAANEALKVRLLETEGIKEVLIAEEEHSAYVKIDSKVTNRFEVEQA
                     IRQA"
     gene            complement(6630..7121)
                     /gene="yajQ"
                     /locus_tag="NBI74_000953"
     CDS             complement(6630..7121)
                     /gene="yajQ"
                     /locus_tag="NBI74_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414960.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide binding protein YajQ"
                     /protein_id="HCD1931322.1"
                     /translation="MPSFDIVSEVDLQEARNAVDNASREVESRFDFRNVEASFELNDA
                     SKTIKVLSESDFQVNQLLDILRAKLLKRGIEGSSLDVPENIVHSGKTWFVEAKLKQGI
                     ESATQKKIVKMIKDSKLKVQAQIQGDEIRVTGKSRDDLQAVMAMVRGGDLGQPFQFKN
                     FRD"
     gene            7289..8200
                     /gene="panE"
                     /locus_tag="NBI74_000954"
     CDS             7289..8200
                     /gene="panE"
                     /locus_tag="NBI74_000954"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HCD1931323.1"
                     /translation="MKITVLGCGALGQLWLTALCKQGHDVQGWLRVPQPYCSVNLVET
                     DGSIFNESLTANDPDFLATSDLLLVTLKAWQVSDAVKSLASTLPVTTPILLIHNGMGT
                     IEELQNIQQPLLMGTTTHAARRDGNVIIHVANGITHIGPARQQDGDYSYLADILQTVL
                     PDVAWHNNIRAELWRKLAVNCVINPLTAIWNCPNGELRHHPQEIMQICEEVAAVIERE
                     GHHTSAEDLRDYVMQVIDATAENISSMLQDIRALRHTEIDYINGFLLRRARAHGIAVP
                     ENTRLFEMVKRKESEYERIGTGLPRPW"
     gene            8163..8753
                     /gene="yajL"
                     /locus_tag="NBI74_000955"
     CDS             8163..8753
                     /gene="yajL"
                     /locus_tag="NBI74_000955"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein deglycase YajL"
                     /protein_id="HCD1931324.1"
                     /translation="MSASALVCLAPGSEETEAVTTIDLLVRGGIKVTTASVASDGNLA
                     ITCSRGVKLLADAPLVEVADGEYDVIVLPGGIKGAECFRDSTLLVETVKQFHRSGRIV
                     AAICAAPATVLVPHDIFPIGNMTGFPTLKDKIPAEQWQDKRVVWDARVKLLTSQGPGT
                     AIDFGLKIIDLLVGREKAHEVASQLVMAAGIYNYYE"
     gene            complement(8807..10255)
                     /gene="thiI"
                     /locus_tag="NBI74_000956"
     CDS             complement(8807..10255)
                     /gene="thiI"
                     /locus_tag="NBI74_000956"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HCD1931325.1"
                     /translation="MKFIIKLFPEITIKSQSVRLRFIKILTGNIRNVLKHYDETLAVV
                     RHWDNIEVRAKDENQRLAIRDALTRIPGIHHILEVEDVPFTDMHDIFEKALVQYRDQL
                     EGKTFCVRVKRRGKHDFSSIDVERYVGGGLNQHIESARVKLTNPEVTVHLEVEDDRLL
                     LIKGRYEGIGGFPIGTQEDVLSLISGGFDSGVSSYMLMRRGCRVHYCFFNLGGAAHEI
                     GVRQVAHYLWNRFGSSHRVRFVAINFEPVVGEILEKIDDGQMGVILKRMMVRAASKVA
                     ERYGVQALVTGEALGQVSSQTLTNLRLIDNVSDTLILRPLISYDKEHIINLARQIGTE
                     DFARTMPEYCGVISKSPTVKAVKSKIEAEEEKFDFSILDKVVEEANNVDIREIAQQTE
                     QEVVEVETVNGFGPNDVILDIRSIDEQEDKPLKVEGIDVVSLPFYKLSTKFGDLDQSK
                     NWLLWCERGVMSRLQALYLREQGFNNVKVYRP"
     gene            10461..10703
                     /gene="xseB"
                     /locus_tag="NBI74_000957"
     CDS             10461..10703
                     /gene="xseB"
                     /locus_tag="NBI74_000957"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HCD1931326.1"
                     /translation="MPKKNEAPASFEKALSELEQIVTRLESGDLPLEEALNEFERGVQ
                     LARQGQAKLQQAEQRVQILLSDNEDASLTPFTPDNE"
     gene            10703..11602
                     /gene="ispA"
                     /locus_tag="NBI74_000958"
     CDS             10703..11602
                     /gene="ispA"
                     /locus_tag="NBI74_000958"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl diphosphate synthase"
                     /protein_id="HCD1931327.1"
                     /translation="MDFPQQLEACVKQANQALSRFIAPLPFQNTPVVETMQYGALLGG
                     KRLRPFLVYATGHMFGVSTNTLDAPAAAVECIHAYSLIHDDLPAMDDDDLRRGLPTCH
                     VKFGEANAILAGDALQTLAFSILSDADMPEVSDRDRISMISELASASGIAGMCGGQAL
                     DLDAEGKHVPLDALERIHRHKTGALIRAAVRLGALSAGDKGRRALPVLDKYAESIGLA
                     FQVQDDILDVVGDTATLGKRQGADQQLGKSTYPALLGLEQARKKARDLIDDARQSLKQ
                     LTEQSFDTSALEALADYIIQRNK"
     gene            11627..13489
                     /gene="dxs"
                     /locus_tag="NBI74_000959"
     CDS             11627..13489
                     /gene="dxs"
                     /locus_tag="NBI74_000959"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HCD1931328.1"
                     /translation="MSFDIAKYPTLALVDSTQELRLLPKESLPKLCDELRRYLLDSVS
                     RSSGHFASGLGTVELTVALHYVYNTPFDQLIWDVGHQAYPHKILTGRRDKIGTIRQKG
                     GLHPFPWRGESEYDVLSVGHSSTSISAGIGIAVAAEKEGKNRRTVCVIGDGAITAGMA
                     FEAMNHAGDIRPDMLVVLNDNEMSISENVGALNNHLAQLLSGKLYSSLREGGKKVFSG
                     VPPIKELLKRTEEHIKGMVVPGTLFEELGFNYIGPVDGHDVLGLITTLKNMRDLKGPQ
                     FLHIMTKKGRGYEPAEKDPITFHAVPKFDPSSGCLPKSSGGLPSYSKIFGDWLCETAA
                     KDNKLMAITPAMREGSGMVEFSRKFPDRYFDVAIAEQHAVTFAAGLAIGGYKPIVAIY
                     STFLQRAYDQVLHDVAIQKLPVLFAIDRAGIVGADGQTHQGAFDLSYLRCIPEMVIMT
                     PSDENECRQMLYTGYHYNDGPSAVRYPRGNAVGVELTPLEKLPIGKGIVKRRGEKLAI
                     LNFGTLMPEAAKVAESLNATLVDMRFVKPLDEALILEMAASHEALVTVEENAIMGGAG
                     SGVNEVLMAHRKPVPVLNIGLPDFFIPQGTQEEMRAELGLDATGMEAKIKAWLA"
     gene            13544..14518
                     /gene="yajO"
                     /locus_tag="NBI74_000960"
     CDS             13544..14518
                     /gene="yajO"
                     /locus_tag="NBI74_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxyxylulose-5-phosphate synthase YajO"
                     /protein_id="HCD1931329.1"
                     /translation="MQYNPLGKTDLRVSRLCLGCMTFGEPDRGNHAWTLPEESSRPII
                     KRALEGGINFFDTANSYSDGSSEEIVGRALRDFARREDVVVATKVFHRVGDLPEGLSR
                     AQILRSIDDSLRRLGMDYVDILQIHRWDYNTPIEETLEALNDVVKVGKARYIGASSMH
                     ASQFAQALELQKQHGWAQFVSMQDHYNLIYREEEREMLPLCYQEGVAVIPWSPLARGR
                     LTRPWGETTARLVSDEVGKNLYQESDENDAQIAERLTGVSEELGATRAQVALAWLLSK
                     PGIAAPIIGTSREEQLDELLNAVDITLKPEQIAELETPYKPHPVVGFK"
     gene            complement(14572..15090)
                     /gene="pgpA"
                     /locus_tag="NBI74_000961"
     CDS             complement(14572..15090)
                     /gene="pgpA"
                     /locus_tag="NBI74_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HCD1931330.1"
                     /translation="MTILPRHKDVAKSRLKMSNPWHLLAVGFGSGLSPIVPGTMGSLA
                     AIPFWYLMTFLPWQLYSLVVMLGICIGVYLCHQTAKDMGVHDHGSIVWDEFIGMWITL
                     MALPTNDWQWVTAGFVIFRILDMWKPWPIRWFDRNVHGGMGIMIDDIVAGVISAVILY
                     FIGHHWPLGILS"
     gene            complement(15068..16045)
                     /gene="thiL"
                     /locus_tag="NBI74_000962"
     CDS             complement(15068..16045)
                     /gene="thiL"
                     /locus_tag="NBI74_000962"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HCD1931331.1"
                     /translation="MACGEFSLIARYFDRVRSSRLDVELGIGDDCALLNIPEKQTLAI
                     STDTLVAGNHFLPDIDPADLAYKALAVNLSDLAAMGADPAWLTLALTLPDVDEAWLES
                     FSDSLFDLLNYYDMQLIGGDTTRGPLSMTLGIHGFVPMGRALTRSGAKPGDWIYVTGT
                     PGDSAAGLAILQNRLQVADAKDADYLIKRHLRPSPRILQGQALRDLANSAIDLSDGLI
                     SDLGHIVKASDCGARIDLALLPFSDALSRHVEPEQALRWALSGGEDYELCFTVPELNR
                     GALDVALGHLGVPFTCIGQMTADIEGLCFIRDGEPVTLDWKGYDHFATP"
     gene            complement(16123..16542)
                     /gene="nusB"
                     /locus_tag="NBI74_000963"
     CDS             complement(16123..16542)
                     /gene="nusB"
                     /locus_tag="NBI74_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HCD1931332.1"
                     /translation="MKPAARRRARECAVQALYSWQLSQNDIADVEYQFLAEQDVKDVD
                     VLYFRELLAGVATNTAYLDGLMKPYLSRLLEELGQVEKAVLRIALYELSKRSDVPYKV
                     AINEAIELAKSFGAEDSHKFVNGVLDKAAPVIRPNKK"
     gene            complement(16562..17032)
                     /gene="ribE"
                     /locus_tag="NBI74_000964"
     CDS             complement(16562..17032)
                     /gene="ribE"
                     /locus_tag="NBI74_000964"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HCD1931333.1"
                     /translation="MNIIEANVATPDARVAITIARFNNFINDSLLEGAIDALKRIGQV
                     KDENITVVWVPGAYELPLAAGALAKTGKYDAVIALGTVIRGGTAHFEYVAGGASNGLA
                     HVAQDSEIPVAFGVLTTESIEQAIERAGTKAGNKGAEAALTALEMINVLKAIKA"
     gene            complement(17121..18224)
                     /gene="ribD"
                     /locus_tag="NBI74_000965"
     CDS             complement(17121..18224)
                     /gene="ribD"
                     /locus_tag="NBI74_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HCD1931334.1"
                     /translation="MQDEYYMARALKLAQRGRFTTHPNPNVGCVIVKDGEIVGEGYHQ
                     RAGEPHAEVHALRMAGEKAKGATAYVTLEPCSHHGRTPPCCDALIAAGVARVVAAMQD
                     PNPQVAGRGLYRLQQAGIDVSHGLMMSEAEQLNKGFLKRMRTGFPYIQLKLGASLDGR
                     TAMASGESQWITSPQARRDVQRLRAQSHAILTSSATVLADDPALTVRWSELDEQTQGL
                     YPQQNLRQPVRIVIDSQNRVTPEHRIVQQPGETWFARTQEDSREWPETVRTLLIPEHK
                     GHLDLVVLMMQLGKQQINSIWVEAGPTLAGALLQAGLVDELIVYIAPKLLGNDARGLC
                     TLPGLEKLADAPQFKFKEIRHVGPDVCLHLVGA"
     gene            complement(18228..18677)
                     /gene="nrdR"
                     /locus_tag="NBI74_000966"
     CDS             complement(18228..18677)
                     /gene="nrdR"
                     /locus_tag="NBI74_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HCD1931335.1"
                     /translation="MHCPFCFAVDTKVIDSRLVGEGSSVRRRRQCLVCNERFTTFEVA
                     ELVMPRVVKSNDVREPFNEEKLRSGMLRALEKRPVSSDDVEMAINHIKSQLRATGERE
                     VPSKMIGNLVMEQLKKLDKVAYIRFASVYRSFEDIKEFGEEIARLED"
     gene            18828..19367
                     /locus_tag="NBI74_000967"
     CDS             18828..19367
                     /locus_tag="NBI74_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HCD1931336.1"
                     /translation="MNTNVFRLLLLGSLFSLSACVQQSEVRQMKHSVSTLNQEMTQLN
                     KETVKITQQNKLNAKSSSGVYLLPGAKTPARLESQIGTLRMSLVNITPDTDGTTLTLR
                     IQGESNDPLPAFSGTVEYGQIQGTIDNFQEINVQNQLINAPASVLAPSDVDIPLQLKG
                     ISVDQLGFVRIHDIQPVMQ"
     gene            19666..20550
                     /gene="tsx"
                     /locus_tag="NBI74_000968"
     CDS             19666..20550
                     /gene="tsx"
                     /locus_tag="NBI74_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-specific channel-forming protein Tsx"
                     /protein_id="HCD1931337.1"
                     /translation="MKKTLLAAGAVLALSSSFTVNAAENDKPQYLSDWWHQSVNVVGS
                     YHTRFGPQLRNDTYLEYEAFAKKDWFDFYGYADAPVFFGGNSDAKGIWNHGSPLFMEI
                     EPRFSIDKLTNTDLSFGPFKEWYFANNYIYDMGRNKDGRQSTWYMGLGTDIDTGLPMS
                     LSMNVYAKYQWQNYGAANENEWDGYRFKVKYFVPITDLWGGQLSYIGFTNFDWGSDLG
                     DDSGYANNGIKTRTNNSIASSHILALNYDHWHYSVVARYWHNGGQWNDDAELNFGNGN
                     FNVRSTGWGGYLVVGYNF"
     gene            complement(20588..20935)
                     /locus_tag="NBI74_000969"
     CDS             complement(20588..20935)
                     /locus_tag="NBI74_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH nuclease family protein"
                     /protein_id="HCD1931338.1"
                     /translation="MAIIPKNYARLESGYREKALKIYPWVCGRCSREFVYSNLRELTV
                     HHIDHDHTNNPEDGSNWELLCLYCHDHEHSKYTEADQYGTTVIAGEDAQKDVGEAKYN
                     PFADLKAMMNKKK"
     gene            complement(21065..22036)
                     /gene="secF"
                     /locus_tag="NBI74_000970"
     CDS             complement(21065..22036)
                     /gene="secF"
                     /locus_tag="NBI74_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HCD1931339.1"
                     /translation="MAQEYTVEQLNHGRKVYDFMRWDYWAFGISGLLLIAAIVIMGVR
                     GFNWGLDFTGGTVIEITLEKPAEIDVMRDALQKAGFEEPMLQNFGSSHDIMVRMPPAE
                     GETGGQVLGSQVLKVINESTNQNAAVKRIEFVGPSVGADLAQTGAMALMAALLSILVY
                     VGFRFEWRLAAGVVIALAHDVIITLGILSLFHIEIDLTIVASLMSVIGYSLNDSIVVS
                     DRIRENFRKIRRGTPYEIFNVSLTQTLHRTLITSGTTLMVILMLYLFGGPVLEGFSLT
                     MLIGVSIGTASSIYVASALALKLGMKREHMLQQKVEKEGADQPSILP"
     gene            complement(22047..23894)
                     /gene="secD"
                     /locus_tag="NBI74_000971"
     CDS             complement(22047..23894)
                     /gene="secD"
                     /locus_tag="NBI74_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HCD1931340.1"
                     /translation="MLNRYPLWKYVMLIVVIVIGLLYALPNLFGEDPAVQITGARGVA
                     ASEQTLIQVQKTLQEEKITAKSVALEEGAILARFDSTDTQLRAREALMGVMGDKYVVA
                     LNLAPATPRWLAAIHAEPMKLGLDLRGGVHFLMEVDMDTALGKLQEQNIDSLRSDLRE
                     KGIPYTTVRKENNYGLSITFRDAKARDEAIAYLSKRHPDLVISSQGSNQLRAVMSDAR
                     LSEAREYAVQQNINILRNRVNQLGVAEPVVQRQGADRIVVELPGIQDTARAKEILGAT
                     ATLEFRLVNTNVDQAAAASGRVPGDSEVKQTREGQPVVLYKRVILTGDHITDSTSSQD
                     EYNQPQVNISLDSAGGNIMSNFTKDNIGKPMATLFVEYKDSGKKDANGRAVLVKQEEV
                     INIANIQSRLGNSFRITGINNPSEARQLSLLLRAGALIAPIQIVEERTIGPTLGMQNI
                     EQGLEACLAGLLVSILFMIIFYKKFGLIATSALIANLILIVGIMSLLPGATLSMPGIA
                     GIVLTLAVAVDANVLINERIKEELSNGRTVQQAIDEGYRGAFSSIFDANITTLIKVII
                     LYAVGTGAIKGFAITTGIGVATSMFTAIVGTRAIVNLLYGGKRVKKLSI"
     gene            complement(23922..24254)
                     /gene="yajC"
                     /locus_tag="NBI74_000972"
     CDS             complement(23922..24254)
                     /gene="yajC"
                     /locus_tag="NBI74_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HCD1931341.1"
                     /translation="MSFFISDAVAATGAPAQGSPMSLILMLVVFGLIFYFMILRPQQK
                     RTKEHKKLMDSIAKGDEVLTNGGLVGRVTKVAENGYIAIALNDTTEVVIKRDFVAAVL
                     PKGTMKAL"
     gene            complement(24277..25404)
                     /gene="tgt"
                     /locus_tag="NBI74_000973"
     CDS             complement(24277..25404)
                     /gene="tgt"
                     /locus_tag="NBI74_000973"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HCD1931342.1"
                     /translation="MKFELDTTDGRARRGRLVFDRGVVETPCFMPVGTYGTVKGMTPE
                     EVEATGAQIILGNTFHLWLRPGQEIMKLHGDLHDFMQWKGPILTDSGGFQVFSLGDIR
                     KITEQGVHFRNPINGDPIFLDPEKSMEIQYDLGSDIVMIFDECTPYPADWDYAKRSME
                     MSLRWAKRSRERFDSLGNKNALFGIIQGSVYEDLRDISVKGLVDIGFDGYAVGGLAVG
                     EPKADMHRILEHVCPQIPADKPRYLMGVGKPEDLVEGVRRGIDMFDCVMPTRNARNGH
                     LFVTDGVVKIRNAKYKSDTGPLDPECDCYTCRNYSRAYLHHLDRCNEILGARLNTIHN
                     LRYYQRLMAGLRKAIEEGKLESFVTDFYQRQGREVPPLNVD"
     gene            complement(25459..26529)
                     /gene="queA"
                     /locus_tag="NBI74_000974"
     CDS             complement(25459..26529)
                     /gene="queA"
                     /locus_tag="NBI74_000974"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HCD1931343.1"
                     /translation="MRVTDFSFELPESLIAHYPMPERSSCRLLSLDGPTGALTHGTFT
                     DLLDKLNPGDLLVFNNTRVIPARLFGRKASGGKIEVLVERMLDDKRILAHIRASKAPK
                     PGAELLLGDDESINATMTARHGALFEVEFNDQRSVLDILNSIGHMPLPPYIDRPDEDA
                     DRELYQTVYSEKPGAVAAPTAGLHFDEPLLEKLRAKGVEMAFVTLHVGAGTFQPVRVD
                     TIEDHIMHSEYAEVPQDVVDAVLAAKARGNRVIAVGTTSVRSLESAAQAAKNDLIEPF
                     FDDTQIFIYPGFQYKVVDALVTNFHLPESTLIMLVSAFAGYQHTMNAYKAAVEEKYRF
                     FSYGDAMFITYNPQAINERVGE"
     gene            26622..27203
                     /gene="acpH"
                     /locus_tag="NBI74_000975"
     CDS             26622..27203
                     /gene="acpH"
                     /locus_tag="NBI74_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP phosphodiesterase"
                     /protein_id="HCD1931344.1"
                     /translation="MNFLAHLHLAHLAESSLSGNLLADFVRGNPEESFPPDVVAGIHM
                     HRRIDVLTDNLPEVREAREWFRNETRRVAPITLDVMWDHFLSRHWSQLSPDFPLQEFT
                     CYAREQVMTILPDSPPRFINLNNYLWSEQWLVRYRDMDFIQSVLNGMASRRPRLDALR
                     DSWYDLDAHYDALETRFWQFYPRMMEQASRKAL"
     gene            complement(27208..29025)
                     /gene="malZ"
                     /locus_tag="NBI74_000976"
     CDS             complement(27208..29025)
                     /gene="malZ"
                     /locus_tag="NBI74_000976"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308480.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin glucosidase"
                     /protein_id="HCD1931345.1"
                     /translation="MMLNAWHLPVPPFVKQSKDQLLITLWLTGEDPPQRIMLRTEHDN
                     EEMSVPMHKQRSQPQPGVTAWRAAIELSCGQPRRRYSFKLLWHDRQRWFTPQGFSRMP
                     PARQEQFAIDVPDIGPQWAADQIFYQIFPDRFARNLPREAEQDHVYYHHAAGQEIILR
                     DWDEPVTAQAGGSTFYGGDLDGISEKLPYLKKLGVTALYLNPVFKAPSVHKYDTEDYR
                     HVDPQFGGDGALLRLRHNTQQLGIRLVLDGVFNHSGDSHAWFDRHNRGTGGACHNPES
                     PWRDWYSFSDDGTALDWLGYASLPKLDYQSESLVNEIYRGEDSIVRHWLKAPWNMDGW
                     RLDVVHMLGEAGGARNNLQHVAGITEAAKETQPDAYIVGEHFGDARQWLQADVEDAAM
                     NYRGFTFPLWGFLANTDISYDPQQIDAQTCMAWMDNYRAGLSHQQQLRMFNQLDSHDT
                     ARFKTLLGRDVARLPLAVVWLFTWPGVPCIYYGDEVGLDGKNDPFCRKPFPWQVEKQD
                     SALFALYQRMIALRKKSQALRRGGCQVLYAEDNVVVFVRVLNQQRVLVAINRGEACEV
                     VLPASPLLNVAQWQRKEGHGQLTDGILALPAISATVWMN"
     gene            complement(29181..30554)
                     /gene="proY"
                     /locus_tag="NBI74_000977"
     CDS             complement(29181..30554)
                     /gene="proY"
                     /locus_tag="NBI74_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline-specific permease ProY"
                     /protein_id="HCD1931346.1"
                     /translation="MESKNKLKRGLSTRHIRFMALGSAIGTGLFYGSADAIKMAGPSV
                     LLAYIIGGIAAYIIMRALGEMSVHNPAASSFSRYAQENLGPLAGYITGWTYCFEILIV
                     AIADVTAFGIYMGVWFPTVPHWIWVLSVVLIICAVNLMSVKVFGELEFWFSFFKVATI
                     IIMIVAGFGIIIWGIGNGGQPTGIHNLWSNGGFFSNGWLGMVMSLQMVMFAYGGIEII
                     GITAGEAKDPEKSIPRAINSVPMRILVFYVGTLFVIMSIYPWNQVGTAGSPFVLTFQH
                     MGITFAASILNFVVLTASLSAINSDVFGVGRMLHGMAEQGSAPKIFSKTSRRGIPWVT
                     VLVMTTALLFAVYLNYIMPENVFLVIASLATFATVWVWIMILLSQIAFRRRLPPEEVK
                     ALKFKVPGGVATTIGGLIFLLFIIGLIGYHPDTRISLYVGFAWIVVLLIGWMFKRRHD
                     RQLAENQ"
     gene            complement(30630..31949)
                     /gene="brnQ"
                     /locus_tag="NBI74_000978"
     CDS             complement(30630..31949)
                     /gene="brnQ"
                     /locus_tag="NBI74_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transporter carrier
                     protein BrnQ"
                     /protein_id="HCD1931347.1"
                     /translation="MTHQLRSRDIIALGFMTFALFVGAGNIIFPPMVGLQAGEHVWTA
                     AFGFLITAVGLPVLTVVALAKVGGGVDSLSTPIGKVAGVLLATVCYLAVGPLFATPRT
                     ATVSFEVGIAPLTGDSALPLFIYSLVYFAIVILVSLYPGKLLDTVGNFLAPLKIIALV
                     ILSVAAIIWPAGSISTATEAYQNAAFSNGFVNGYLTMDTLGAMVFGIVIVNAARSRGV
                     TEARLLTRYTVWAGLMAGVGLTLLYLALFRLGSDSASLVDQSANGAAILHAYVQHTFG
                     GGGSFLLAALIFIACLVTAVGLTCACAEFFAQYVPLSYRTLVFILGGFSMVVSNLGLS
                     QLIQISVPVLTAIYPPCIALVVLSFTRSWWHNSSRVIAPPMFISLLFGILDGIKASAF
                     SDILPSWAQRLPLAEQGLAWLMPTVVMVVLAIIWDRAAGRQVTSSAH"
     gene            complement(32356..33651)
                     /gene="phoR"
                     /locus_tag="NBI74_000979"
     CDS             complement(32356..33651)
                     /gene="phoR"
                     /locus_tag="NBI74_000979"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon sensor histidine kinase PhoR"
                     /protein_id="HCD1931348.1"
                     /translation="MLERLSWKRLVLELLFCCLPAFILGAFLGYLPWFLLASVTGLLI
                     WHFWNLLRLSWWLWVDRSMTPPPGRGSWEPLLYGLHQMQLRNKKRRRELGNLIKRFRS
                     GAESLPDAVVLTTEEGGIFWCNGLAQQILGLRWPEDNGQNILNLLRYPEFTQYLKTRD
                     FSRPLNLVLNTGRHLEIRVMPYTHKQLLMVARDVTQMHQLEGARRNFFANVSHELRTP
                     LTVLQGYLEMMDEQPLEGAVREKALHTMREQTQRMEGLVKQLLTLSKIEAAPTHLLNE
                     KVDVPMMLRVVEREAQTLSQKKQTFTFEIDNGLKVSGNEDQLRSAISNLVYNAVNHTP
                     EGTHITVRWQRVPHGAEFSVEDNGPGIAPEHIPRLTERFYRVDKARSRQTGGSGLGLA
                     IVKHAVNHHESRLNIESTVGKGTRFSFIIPERLIAKNSD"
     gene            complement(33709..34398)
                     /gene="phoB"
                     /locus_tag="NBI74_000980"
     CDS             complement(33709..34398)
                     /gene="phoB"
                     /locus_tag="NBI74_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000113934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate response regulator transcription
                     factor PhoB"
                     /protein_id="HCD1931349.1"
                     /translation="MARRILVVEDEAPIREMVCFVLEQNGFQPVEAEDYDSAVNQLNE
                     PWPDLILLDWMLPGGSGIQFIKHLKRESMTRDIPVVMLTARGEEEDRVRGLETGADDY
                     ITKPFSPKELVARIKAVMRRISPMAVEEVIEMQGLSLDPTSHRVMAGEEPLEMGPTEF
                     KLLHFFMTHPERVYSREQLLNHVWGTNVYVEDRTVDVHIRRLRKALEPGGHDRMVQTV
                     RGTGYRFSTRF"
     gene            34588..35790
                     /gene="sbcD"
                     /locus_tag="NBI74_000981"
     CDS             34588..35790
                     /gene="sbcD"
                     /locus_tag="NBI74_000981"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcD"
                     /protein_id="HCD1931350.1"
                     /translation="MRILHTSDWHLGQNFYSKSREAEHQAFLDWLLETAQAHLVDAII
                     VAGDVFDTGSPPSYARTLYNRFVVNLQQTGCHLVVLAGNHDSVATLNESRDIMAFLNT
                     TVVASAGHAPQILPRRDGTPGAVLCPIPFLRPRDIITSQAGLNGIEKQQHLLAAITDY
                     YQQHYADACKLRGDQPLPIIATGHLTTVGASKSDAVRDIYIGTLDAFPAQNFPPADYI
                     ALGHIHRAQLIGGMEHVRYCGSPIPLSFDECGKSKYVHLVTFSNGKLESVENLNVPVT
                     QPMAVLKGDLASITAQLEQWRDVSQEPPVWLDIEITTDEYLHDIQRKIQALTESLPVE
                     VLLVRRSREQRERVLASQQRETLSELSVEEVFNRRLALEELDESQQQRLQHLFATTLH
                     SLAGEHEA"
     gene            35787..38930
                     /gene="sbcC"
                     /locus_tag="NBI74_000982"
     CDS             35787..38930
                     /gene="sbcC"
                     /locus_tag="NBI74_000982"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcC"
                     /protein_id="HCD1931351.1"
                     /translation="MKILSLRLKNLNSLKGEWKIDFTREPFASNGLFAITGPTGAGKT
                     TLLDAICLALYHETPRLSNVSQSQNDLMTRDTAECLAEVEFEVKDEAYRAFWSQNRAR
                     NQPDGNLQVPRVELARCADGKILADKVKDKLELTATLTGLDYGRFTRSMLLSQGQFAA
                     FLNAKPKERAELLEELTGTEIYGQISAMVFEQHKSARTELEKLQAQASGVALLTPEQV
                     QSLTASLQVLTDEEKQLITAQQQEQQSLNWLTRLDELQQEASRRQQALQQALAEEEQA
                     QPQLAALSLAQPARNLRPHWERIAEHSTALAHTRQQIEEVNTRLQSTMALRASIRHHA
                     AKQSAELQQQQQSLNAWLQEHDRFRQWNNELAGWRAQFSQQTSDREHLRQWQQQLTHA
                     EQKLNALAAITLTLTADEVASAQAQHAEQRPLRQRLVALHGQIVPQQKRLAQLQVAIQ
                     NVTLEQTQRNAALNKMRHRYKEKMQQLADVKTICEQEARIKTLEAQRAQLQAGQPCPL
                     CGSTSHPAVEAYQALEPGVNQARLLTLEKEVKKLGEEGATLRGQLDALTKQLQRDENE
                     AQSLRQDEQALTQQWQAVTASLNITLQPQDDIQPWLDAQDEHERQLRLLSQRHELQGQ
                     IAAHNQQIIQYQQQIEQRQQQLLTALAGYALTLPQEDEEESWLATRQQEAQSWQQRQN
                     ELTALQNRMQQLTPILETLPQSDELPHSEETVALENWRQVHEQCLALHSQQQTLQQQD
                     VLAAQSLQKAQAQFDTALQASVFDDQQAFLAALMDEQTLTQLEQLKQNLENQRRQAQT
                     LVTQTAETLTQHQQHRPGGLSLTVTVEQIQQELAQTHQKLRENTTSQGEIRQQLKQDA
                     DNRQQQQTLMQQIAQMTQQVEDWGYLNSLIGSKEGDKFRKFAQGLTLDNLVHLANQQL
                     TRLHGRYLLQRKASEALEVEVVDTWQADAVRDTRTLSGGESFLVSLALALALSDLVSH
                     KTRIDSLFLDEGFGTLDSETLDTALDALDALNASGKTIGVISHVEAMKERIPVQIKVK
                     KINGLGYSKLESAFAVK"
     gene            39056..40240
                     /gene="araJ"
                     /locus_tag="NBI74_000983"
     CDS             39056..40240
                     /gene="araJ"
                     /locus_tag="NBI74_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414930.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter AraJ"
                     /protein_id="HCD1931352.1"
                     /translation="MKKVILSLALGTFGLGMAEFGIMGVLTELAHNVGISIPAAGHMI
                     SYYALGVVVGAPIIALFSSRYSLKHILLFLVALCVIGNAMFTLSSSYLMLAIGRLVSG
                     FPHGAFFGVGAIVLSKIIKPGKVTAAVAGMVSGMTVANLLGIPLGTYLSQEFSWRYTF
                     LLIAIFNIAVMASVYFWVPDIRDEAKGKLREQFHFLRSPAPWLIFAATMFGNAGVFAW
                     FSYVKPYMMFISGFSETAMTFIMMLVGLGMVLGNMLSGRISGRYSPLRIAAVTDFIIV
                     LALLMLFFFGGMKTTSLIFAFICCAGLFALSAPLQILLLQNAKGGELLGAAGGQIAFN
                     LGSAVGAYCGGMMLTLGLAYNYVALPAALLSFAAMSSLLLYGRYKRQQAADSPVLAKP
                     LG"
     gene            complement(40509..41417)
                     /gene="mak"
                     /locus_tag="NBI74_000984"
     CDS             complement(40509..41417)
                     /gene="mak"
                     /locus_tag="NBI74_000984"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase"
                     /protein_id="HCD1931353.1"
                     /translation="MRIGIDLGGTKTEVIALGDAGEQLYRHRLPTPRDDYRQTIETIA
                     TLVDMAEQATGQRGTVGMGIPGSISPYTGVVKNANSTWLNGQPFDKDLSARLQREVRL
                     ANDANCLAVSEAVDGAAAGAQTVFAVIIGTGCGAGVAFNGRAHIGGNGTAGEWGHNPL
                     PWMDEDELRYREEVPCYCGKQGCIETFISGTGFATDYRRLSGHALKGSEIIRLVEESD
                     PVAELALRRYELRLAKSLAHVVNILDPDVIVLGGGMSNVDRLYQTVPQLIKQFVFGGE
                     CETPVHKAKHGDSSGVRGAAWLWPQE"
     gene            41542..42453
                     /gene="rdgC"
                     /locus_tag="NBI74_000985"
     CDS             41542..42453
                     /gene="rdgC"
                     /locus_tag="NBI74_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HCD1931354.1"
                     /translation="MLWFKNLMVYRLSREISLRAEEMEKQLASMAFTPCGSQDMAKMG
                     WVPPMGSHSDALTHVANGQIVICARKEEKILPSPVIKQALEAKIAKLEAEQVRKLKKT
                     EKDSLKDEVLHSLLPRAFSRFSQTMMWIDTVNGLIMVDCASAKKAEDTLALLRKSLGS
                     LPVVPLSMENPIELTLTEWVRSGSAAQGFQLLDEAELKSLLEDGGVIRAKKQDLTSEE
                     ITNHIEAGKVVTKLALDWQQRIQFVMCDDGSLKRLKFCDELRDQNEDIDREDFAQRFD
                     ADFILMTGELAALIQNLIEGLGGEAQR"
     gene            complement(42534..42614)
                     /locus_tag="NBI74_000986"
     CDS             complement(42534..42614)
                     /locus_tag="NBI74_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1931355.1"
                     /translation="MTQRPWSKLQHKTHNIAALRIIARCS"
     gene            complement(42611..42952)
                     /locus_tag="NBI74_000987"
                     /pseudo
     CDS             complement(42611..42952)
                     /locus_tag="NBI74_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308469.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2773 domain-containing protein"
     gene            complement(43100..43384)
                     /gene="ppnP"
                     /locus_tag="NBI74_000988"
     CDS             complement(43100..43384)
                     /gene="ppnP"
                     /locus_tag="NBI74_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine/purine nucleoside phosphorylase"
                     /protein_id="HCD1931356.1"
                     /translation="MLQSNEYFSGKVKSIGFSSSSTGRASVGVMVEGEYTFSTAEPEE
                     MTVISGALNVLLPDATDWQVYEAGSVFNVPGHSEFHLQVAEPTSYLCRYL"
     gene            complement(43456..44133)
                     /locus_tag="NBI74_000989"
     CDS             complement(43456..44133)
                     /locus_tag="NBI74_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AroM family protein"
                     /protein_id="HCD1931357.1"
                     /translation="MSASLAILTIGIVPMQEVLPLLTEYIDEDNISHHSLLGKLSREE
                     VMAEYAPEAGEDTILTLLNDNHLAHVSRRKVERDLQGVVEVLDNQGYDVIILMSTANI
                     SSMTARNTIFLEPSRILPPLVSSIVEDHQVGVIVPVEELLTVQAQKWQILQKPPVFSL
                     GNPIHDSEQKIIDAGKELLAKGADVIMLDCLGFNQRHRDLLQKQLDVPVLLSNVLIAR
                     LAAELLM"
     gene            complement(44391..44582)
                     /gene="yaiA"
                     /locus_tag="NBI74_000990"
     CDS             complement(44391..44582)
                     /gene="yaiA"
                     /locus_tag="NBI74_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YaiA"
                     /protein_id="HCD1931358.1"
                     /translation="MPTKPPYPREAYIVTIEKGKPGQTVTWYQLRADHPKPDSLISEH
                     PTAQEAMDAKKRYEDPDKE"
     gene            complement(44632..45156)
                     /gene="aroL"
                     /locus_tag="NBI74_000991"
     CDS             complement(44632..45156)
                     /gene="aroL"
                     /locus_tag="NBI74_000991"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroL"
                     /protein_id="HCD1931359.1"
                     /translation="MTQPLFLIGPRGCGKTTVGMALADSLNRRFVDTDQWLQSQLNMT
                     VAEIVEREEWAGFRARETAALEAVTAPSTVIATGGGIILTEFNRHFMQNNGIVVYLCA
                     PVSVLVNRLQAAPEEDLRPTLTGKPLSEEVQEVLEERDALYREVAHIIIDATNEPSQV
                     ISEIRSALAQTINC"
     gene            complement(45339..45797)
                     /locus_tag="NBI74_000992"
     CDS             complement(45339..45797)
                     /locus_tag="NBI74_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HCD1931360.1"
                     /translation="MTIWVDADACPNVIKEILYRAAERMQMPLVLVANQSLRVPPSRF
                     IRTLRVAAGFDVADNEIVRQCEAGDLVITADIPLAAEAIEKGAAALNPRGERYTPATI
                     RERLTMRDFMDTLRASGIQTGGPDSLSQRDRQAFAAELEKWWLEVQRSRG"
     gene            45917..46726
                     /gene="proC"
                     /locus_tag="NBI74_000993"
     CDS             45917..46726
                     /gene="proC"
                     /locus_tag="NBI74_000993"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HCD1931361.1"
                     /translation="MEKKIGFIGCGNMGKAILGGLIASGQVLPGQIWVYTPSPDKVTA
                     LHEQFGINAAESAQEVAQIADIIFAAVKPGIMIKVLSEITSSLNKDSLVVSIAAGITL
                     DQLARALGHDRKIIRAMPNTPALVNAGMTSVTPNALVTPEDTADVLNIFRCFGEAEVI
                     AEPMIHPVVGVSGSSPAYVFMFIEAMADAAVLGGMPRAQAYKFAAQAVMGSAKMVLET
                     GEHPGTLKDMVCSPGGTTIEAVRVLEEKGFRAAVIEAMTKCMEKSEKLSKS"
     gene            complement(46743..47858)
                     /gene="adrA"
                     /locus_tag="NBI74_000994"
                     /gene_synonym="dgcC"
     CDS             complement(46743..47858)
                     /gene="adrA"
                     /locus_tag="NBI74_000994"
                     /gene_synonym="dgcC"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase AdrA"
                     /protein_id="HCD1931362.1"
                     /translation="MFPKIMNDENFFKKAAAHGEEPPLTPQNEHQRSGLRFARRVRLP
                     RAVGLAGMFLPIASTLVSHPPPGWWWLVLVGWAFVWPHLAWQIASRAVDPLSREIYNL
                     KTDAVLAGMWVGVMGVNVLPSTAMLMIMCLNLMGAGGPRLFVAGLVLMVVSCLVTLEL
                     TGITVSFNSAPLEWWLSLPIIVIYPLLFGWVSYQTATKLAEHKRRLQVMSTRDGMTGV
                     YNRRHWETMLRNEFDNCRRHNRDATLLIIDIDHFKSINDTWGHDVGDEAIVALTRQLQ
                     ITLRGSDVIGRFGGDEFAVIMSGTPAESAITAMLRVHEGLNTLRLPNTPQVTLRISVG
                     VAPLNPQMSHYREWLKSADLALYKAKKAGRNRTEVAA"
     gene            complement(47960..48280)
                     /gene="psiF"
                     /locus_tag="NBI74_000995"
     CDS             complement(47960..48280)
                     /gene="psiF"
                     /locus_tag="NBI74_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PsiF"
                     /protein_id="HCD1931363.1"
                     /translation="MKITLLVTLLFGLVFLTTVGAAERTLTPQQQRMTSCNQQATAQA
                     LKGDARKTYMSDCLKNSKSAPGEKSLTPQQQKMRECNNQATQQSLKGDDRNKFMSACL
                     KKAA"
     gene            complement(48399..49814)
                     /gene="phoA"
                     /locus_tag="NBI74_000996"
     CDS             complement(48399..49814)
                     /gene="phoA"
                     /locus_tag="NBI74_000996"
                     /EC_number="3.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HCD1931364.1"
                     /translation="MKQSTIALALLPLLFTPVTKARTPEMPVLENRAAQGDITAPGGA
                     RRLTGDQTAALRDSLSDKPAKNIILLIGDGMGDSEITAARNYAEGAGGFFKGIDALPL
                     TGQYTHYALNKKTGKPDYVTDSAASATAWSTGVKTYNGALGVDIHEKDHPTILEMAKA
                     AGLATGNVSTAELQDATPAALVAHVTSRKCYGPSATSEKCPGNALEKGGKGSITEQLL
                     NARADVTLGGGAKTFAETATAGEWQGKTLREQAQARGYQLVSDAASLNSVTEANQQKP
                     LLGLFADGNMPVRWQGPKATYHGNIDKPAVTCTPNPQRNDSVPTLAQMTDKAIELLSK
                     NEKGFFLQVEGASIDKQDHAANPCGQIGETVDLDEAVQRALEFAKKDGNTLVIVTADH
                     AHASQIVAPDTKAPGLTQALNTKDGAVMVMSYGNSEEDSQEHTGSQLRIAAYGPHAAN
                     VVGLTDQTDLFYTMKAALGLK"
     gene            complement(49915..50175)
                     /gene="iraP"
                     /locus_tag="NBI74_000997"
     CDS             complement(49915..50175)
                     /gene="iraP"
                     /locus_tag="NBI74_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraP"
                     /protein_id="HCD1931365.1"
                     /translation="MKNLIAELLFKLAQKEEESKELCAQVEALEIIVTAMLRNMAQND
                     QQRLIDQVEGALYEVKPDASIPDDDTELLRDYVKKLLRHPRQ"
BASE COUNT        12216 a        13401 c        12681 g        11905 t
ORIGIN      
        1 tgcacttccc tttcgttata acgccctttt gcaacagctt cttaaaatca acttgatatg
       61 ttttgcaaca tatgtgacct gatagccaaa tccaagtaac aggaatttaa tcatgtttac
      121 agtaatgtaa ccttcccgta aaatgcccac acactttaaa cgccaccaga tcccgtggaa
      181 ttgaggtcgt taaatgagac tcaggaaata caataaaagt ttgggatggt tgtcattatt
      241 tgcaggcact gtattgctca gtggctgtaa ttctgcgctg ttagatccca aaggacagat
      301 tggtctggag caacgttcac tgatactgac ggcatttggc ctgatgttga ttgtcgttat
      361 tcccgcaatc ttgatggctg ttggtttcgc ctggaagtac cgtgcgagca ataaagatgc
      421 taagtacagc ccgaactggt cacactccaa taaagtggaa gctgtggtct ggacggtacc
      481 tatcttaatc atcatcttcc ttgcggtact gacctggaaa accactcacg ctcttgagcc
      541 tagcaagccg ctggcacacg acgagaagcc cattaccatc gaagtggttt ccatggactg
      601 gaaatggttc ttcatctacc cggaacaggg cattgcaacc gtgaatgaaa tcgctttccc
      661 ggcgaacact ccggtgtact tcaaagtgac ctccaactcc gtgatgaact ccttcttcat
      721 tccgcgtctg ggtagccaga tttatgccat ggccggtatg cagactcgcc tgcatctgat
      781 cgccaacgaa cccggtactt atgacggtat ctccgccagc tacagcggcc cgggcttctc
      841 aggcatgaag ttcaaagcta ttgcaacacc ggatcgcgcc gcattcgacc aatgggtcgc
      901 aaaagcgaaa cagtcgccga acagcatgtc tgacatggca gcgttcgaaa aactggccgc
      961 gcctagcgaa tacaaccagg tggaatattt ctccaacgtg aaaccagact tgtttgccga
     1021 tgtgattaac aagtttatgg ctcacggtaa gagcatggac atgacccagc cagaaggtga
     1081 gcacagcgca catgaaggta tggaaggcat ggacatgagc cacgcggaat ccgcccatta
     1141 aaggggttga ggaagaataa agatgttcgg aaaattatca cttgatgcag tcccgttcca
     1201 tgaacctatc gtcatggtta cgatcgctgg cattattttg ggaggtctgg cgctcgttgg
     1261 cctgatcact tacttcggta agtggaccta cctgtggaaa gagtggctga cctccgttga
     1321 ccataaacgt ctcggtatca tgtatatcat cgtggcgatt gtgatgttgc tgcgtggttt
     1381 tgctgacgcc attatgatgc gtagtcagca ggcccttgcc tctgcgggcg aagcgggctt
     1441 cctgccacct caccactacg atcagatctt caccgcacac ggcgtgatca tgatcttctt
     1501 cgtggcgatg cctttcgtta tcggtctgat gaacctggtg gttccgctgc agatcggcgc
     1561 gcgtgacgtt gcgttcccgt tcctcaacaa cttaagcttc tggtttaccg ttgttggtgt
     1621 gattctggtt aacgtttctc tcggcgtggg cgaatttgcg cagaccggct ggctggccta
     1681 tccaccgcta tcgggaatag agtacagtcc gggagtcggt gtcgattact ggatatggag
     1741 tctccagcta tccggtatag gtacgacgct taccggtatc aacttcttcg ttaccattct
     1801 gaagatgcgc gcaccgggca tgaccatgtt caagatgcca gtatttacct gggcatcact
     1861 gtgcgcaaac gtactgatta ttgcttcctt cccaattctg acggttaccg tcgcgttgtt
     1921 gaccctggat cgctatctgg gcacccattt ctttaccaac gatatgggtg gcaacatgat
     1981 gatgtatatc aacctgattt gggcctgggg ccacccggaa gtttacatct tgatcctgcc
     2041 tgtattcggt gtgttctccg aaattgcggc aaccttctcg cgtaaacgtc tgtttggtta
     2101 tacctcgctg gtatgggcaa ccgtctgtat caccgtgctg tcgttcatcg tctggctgca
     2161 ccacttcttt acgatgggtg cgggcgcgaa cgtaaacgcc ttctttggta tcaccacgat
     2221 gattatcgcc atccctaccg gggtgaagat cttcaactgg ctgttcacca tgtatcaggg
     2281 ccgcatcgtg ttccattctg cgatgctgtg gaccatcggt tttatcgtta ccttctcggt
     2341 aggcggtatg actggcgtgc tgctggcagt accgggcgcg gacttcgttc tgcataacag
     2401 cctgttcctg attgcgcact tccataacgt gatcatcggc ggcgtggtct tcggctgctt
     2461 cgcagggatg acctactggt ggcctaaagc gttcggtttc aaactgaacg aaacctgggg
     2521 taaacgcgcg ttctggttct ggatcatcgg cttcttcgtt gcctttatgc cactgtatgc
     2581 gctgggcttc atgggcatga cccgtcgttt gagccagcag attgacccgc agttccacac
     2641 catgctgatg attgcagcca gcggtgcggt actgattgcg ctgggtattc tctgcctcgt
     2701 tattcagata tacgtttcta ttcgtgaccg cgaccagaac cgtgacctga ctggcgaccc
     2761 gtggggtggc cgtacgctgg agtgggcaac ctcttccccg cctccgttct ataactttgc
     2821 cgttgtgccg cacgttcacg aacgtgatgc attctgggaa atgaaagaga aaggcgaagc
     2881 gtacaaaaag cctgaccact atgaagaaat tcatatgccg aaaaacagcg gtgccggtat
     2941 cgtcattgcg gctttctcca ccatcttcgg tttcgccatg atctggcata tctggtggct
     3001 agcgattgtt ggcttcgcag gcatgatcat cacctggatc gtgaaaagct tcgacgagga
     3061 cgtggattac tacgtgccgg tggcagaaat cgaaaaactg gaaaaccagc atttcgatga
     3121 gattactaag gcagggctga aaaatggcaa ctgatacttt gacgcacgcg actgcccacg
     3181 cgcacgaaca cgggcaccac gatgcaggcg gaaccaaaat tttcggattt tggatctacc
     3241 tgatgagcga ctgcattctg ttctctatct tgtttgctac ctatgccgtt ctggtgaacg
     3301 gcaccgcagg cggcccgaca ggtaaggaca ttttcgaact gccgttcgtt ctggttgaaa
     3361 ctttcttgct gttgttcagc tccatcacct atggcatggc ggctatcgcc atgtacaaaa
     3421 acaacaaaag ccaggtgatc tcctggctgg cgttgacatg gttgtttggt gccggattta
     3481 tcgggatgga aatctatgaa ttccatcacc tgattgttaa cggcatgggt ccggatcgca
     3541 gcggcttcct gtcagcattc tttgcgctgg tcggcacgca cggtctgcac gtcacttccg
     3601 gtcttatctg gatggcggtg ctgatggtgc aaatcgcccg tcgcggcctg accagcacta
     3661 accgtacccg catcatgtgc ctgagcctgt tctggcactt cctggatgtg gtttggatct
     3721 gtgtgttcac tgttgtttat ctgatggggg cgatgtaatg agtcattcta acgcaagcag
     3781 cggcgcatcc catggcagcg taaagaccta catgacaggc tttatcctgt cgataatcct
     3841 gacggtaatt ccgttctgga tggtgatgac aggggctgcc tctccggccg taattctggg
     3901 aacaatcctg gcaatggcag tggtacaaat tctggtgcat ctggtgtgct tcctgcacat
     3961 gaataccaaa tcagatgaag gctggaatat gacggcgttt gtcttcaccg tgctaatcat
     4021 cgccatcctg gttgtgggct ccatctggat tatgtggaac ctcaactaca acatgatgat
     4081 gcactaagag cggcggttat gatatttaag caatacctgc aagtaacgaa accaggcatc
     4141 atctttggca acctgatctc ggtgattggg ggattcttgc tggcctcaaa gggcagcatt
     4201 gattatcccc tgtttatcta cacgctggtt ggggtgtcac tggttgtggc gtcgggttgt
     4261 gtgtttaaca actacatcga cagggatatc gacagaaaga tggaaaggac gaagaatcgg
     4321 gtgctggtta aaggcctgat ctctcctgct gtctcgctgg tgtacgccac cttgctgggt
     4381 attgctggct ttatgctgct gtggtttggc gcgaatccgc tggcctgctg gctgggggtg
     4441 atgggctttg tggtttatgt cggcgtttat agcctgtaca tgaaacgtca ttctgtctac
     4501 ggcacattga ttggttcgct ctccggcgct gcgcctccgg tgatcggtta ctgtgcggta
     4561 accggcgagt tcgatagcgg cgcagcgatc ctgttggcta tcttcagcct gtggcagatg
     4621 cctcactctt atgccatagc cattttccgc tttaaggatt atcaggcggc aaacattccg
     4681 gtactgccgg tggtgaaagg catttcggtg gcgaagaatc acatcacgct gtatatcatc
     4741 gcctttgccg ttgccacgct gatgctctct cttggcggct acgctgggta taaatatctg
     4801 gtggtcgccg ctgcggtgag cgtctggtgg ttaggcatgg ctctgcgcgg ctataaagtt
     4861 gctgatgaca gaatctgggc gcgcaagctg ttcggtttct ctatcatcgc tatcactgcc
     4921 ctctcggtga tgatgtccgt tgattttatg gtgccggact cgcatacgct gctggctgct
     4981 gtgtggtaac acaacctctc agttaaaaag gtgctacggc accttttttc ttagcattag
     5041 aatcatatcc ctctcgaaat atttactaaa aaatccgcat gtttacccca ttcgtttgcc
     5101 gctttacact agtcgcgaat ttaaaacaga ggtggtaatg aacgattata aaatgacgcc
     5161 aggtgagcgg cgcgcgacct ggggtttagg gaccgtattt tcattacgca tgctgggcat
     5221 gttcatggtt ctgccggttc tgaccacgta cggcatggct ctgcaaggtg ccagcgaagc
     5281 attaatcggt attgccattg gtatttatgg tctgactcag gccgtttttc agattccgtt
     5341 tggcctgctt tcagaccgca ttggtcgcaa accattaatt gtcggtgggc tggcggtgtt
     5401 tgccgccggt agcgttatcg ctgcgctctc tgactccatc tggggaatta ttctgggccg
     5461 ggcgctacaa ggctccggtg cgattgccgc agccgttatg gcgctgcttt ccgatctcac
     5521 gcgcgaacaa aaccgcacca aagcgatggc gtttatcggc gtgagctttg gcattacctt
     5581 tgccattgcg atggtgcttg gcccgatcat cactcacaaa cttgggctgc acgtgctgtt
     5641 ctggatgatc gctattctgg caacaaccgg cattgcgttg accatttggg ttgtgcccaa
     5701 cagtagcact catgtactta atcgtgagtc cggaatggta aaaggcagtt tcagtaaagt
     5761 gctggcggaa ccgcggctgc tgaaactcaa ctttggcatt atgtgtctgc atattttgct
     5821 gatgtcgacc tttgttgccc tgcccggaca gatggctgat gcggggttcc cggcggctga
     5881 acactggaag gtctacctgg caacgatgtt aatcgccttt ggctcggtcg tgcctttcat
     5941 tatctacgct gaagttaagc gcaaaatgaa gcaagtcttt gtcttctgcg tcgggttgat
     6001 cgtggttgcg gaaattgtgt tgtggaacgc acaaacgcag ttctggcaac tggtggtcgg
     6061 cgtgcagctt ttctttgtgg cgtttaattt gatggaagcc cttctgcctt cacttatcag
     6121 taaagaatcg ccagcaggtt acaaaggtac ggcgatgggt gtttactcca ccagccagtt
     6181 tcttggcgtg gcgattggcg gttcgctggg cggctggatt gacggcatgt ttgacggtca
     6241 gggagtattt ctcgctggcg caatgctggc cgcagtgtgg ctggcagtcg ccagtaccat
     6301 gaaagaaccg ccgtatgtca gcagcttgcg cattgaaatc ccggcgaaca ttgccgcaaa
     6361 cgaggcgtta aaagtgcgtt tgctggaaac tgaaggcatc aaagaagtgt tgattgcaga
     6421 agaagaacat tcagcttatg tgaaaatcga cagcaaagtg acgaatcgct ttgaggttga
     6481 acaggcaatt cgccaggcat aaaagcgagg cgaaattgcc tgctgcgcta cgcttatcag
     6541 gcctactaag ttcctgagtt acaaaccttt gtaggccgaa tcaggcgttc atgccgcatc
     6601 tgacatgaac aaagcgcagg ttgtcgcgat taatcgcgga agtttttgaa ctggaacggc
     6661 tgacccagat cgccaccacg taccatcgcc atgacagcct gtaaatcatc acgagatttg
     6721 cccgtcacac ggatctcatc gccctgaatt tgcgcctgca ctttcagttt gctgtctttg
     6781 atcattttga cgattttctt ctgagtcgcg ctctcaatgc cctgtttcag tttcgcttcc
     6841 acaaaccagg ttttaccgct atgaacgata ttttccggta catccagtga actgccttca
     6901 atgccgcgct tcagcaactt ggcacgcaga atatccagca actgattgac ctggaagtcg
     6961 gactcgctca acactttgat ggttttgctg gcgtcattca gctcaaatga ggcttcaacg
     7021 ttacggaagt caaaacggga ctccacttcg cggctcgcgt tatcgaccgc gttacgtgct
     7081 tcctgaagat caacttcaga gacaatatcg aaagatggca tcttttcctc tcccttcatt
     7141 tttgatgcga agcataatac ctgcaaagtt aaataaccaa cagtcctgca ccaggacgta
     7201 tcccttaatt gtgcatagtc gcaacagcat gcacaattaa gggatagcct tattgaacac
     7261 ccggagtggt tgcgggtgag gaggaacaat gaaaattacc gtattgggat gcggtgcctt
     7321 agggcaatta tggcttacag cactttgtaa acagggtcac gacgttcagg gctggctgcg
     7381 cgtaccgcaa ccttattgta gtgtgaatct ggttgagaca gatggttcga tatttaatga
     7441 atcgctgacc gccaacgatc ccgattttct cgccaccagc gatctgctcc tggtgacgct
     7501 gaaagcatgg caggtttccg atgccgtcaa aagcctcgcg tccacactgc ctgtaaccac
     7561 gccaatactg ttaattcaca acggcatggg caccatcgaa gagttgcaaa acattcagca
     7621 gccattactg atgggcacca ccacccatgc cgcccgccgc gacggcaatg tcattattca
     7681 tgtggcaaac ggtatcacgc atattggtcc ggcacggcaa caggacggcg attacagtta
     7741 tctggcggat attttgcaaa ccgtattgcc tgacgtcgcg tggcataaca atattcgcgc
     7801 cgagctgtgg cgcaagctgg cagtcaactg tgtgattaat ccactgaccg ccatctggaa
     7861 ttgcccgaat ggtgaattac gtcatcatcc gcaagaaatt atgcagatat gcgaagaagt
     7921 cgcggcagtg atcgaacgcg aagggcatca tacttcagca gaagatttgc gtgattacgt
     7981 gatgcaggtg attgatgcaa cagcggaaaa tatctcgtcg atgttgcagg atatccgcgc
     8041 gctgcgccac accgaaatcg actatatcaa tggttttctc ttacgccgcg cccgcgcgca
     8101 tgggattgcc gtaccggaaa acacccgcct gtttgaaatg gtaaaaagaa aggagagtga
     8161 atatgagcgc atcggcactg gtttgcctcg cccctggtag tgaagagact gaagccgtca
     8221 ccactatcga cctcttggtt cgcggcggta tcaaagtcac cactgccagc gtcgccagcg
     8281 atggtaacct ggcgattacc tgctcgcgcg gcgtgaagct gctggcggat gcgccgctgg
     8341 tcgaagtggc tgatggcgaa tatgacgtga ttgtgctgcc tggtggcatc aaaggcgcag
     8401 agtgttttcg cgatagcacc ctgctggttg aaaccgttaa acagttccac cgttccgggc
     8461 gtatcgtcgc ggctatttgc gccgcgccag ccaccgtgct ggtcccgcac gatatcttcc
     8521 cgattggcaa tatgaccggc ttcccaacgc tgaaagacaa aattcccgcc gaacaatggc
     8581 aggacaagcg cgtcgtctgg gatgcacggg taaaattgct gaccagccag gggccgggta
     8641 cagctatcga ctttggtctg aaaattatcg acctgctggt tgggcgcgag aaagcccatg
     8701 aagtggcatc acaactggtg atggcggcag ggatttataa ttattacgag tagtgtcgga
     8761 tgcggtaagc gtcgcatctg accagatgcg acgttacaac cacatattac gggcgataca
     8821 ccttcacatt gttaaagccc tgctcgcgca gatagagcgc ctgcaggcgg ctcatcaccc
     8881 cgcgctcaca ccacaacagc cagtttttgc tctggtcgag atcgccaaat ttggtgctca
     8941 gtttatagaa cggcagagaa accacatcaa tcccttcgac tttcagtggc ttatcttcct
     9001 gttcatcgat agaacggata tcgaggatca cgtcgttcgg gccgaagcca ttgacggttt
     9061 ccacttccac cacttcctgc tcggtctgct gggcgatttc gcggatatca acgttattcg
     9121 cttcctccac cactttatcg agaatgctga agtcgaactt ctcttcttcc gcttcaatct
     9181 tcgatttaac cgctttcacc gtcgggcttt tggaaatcac gccgcaatat tccggcatcg
     9241 tgcgggcaaa gtcttcggtg ccaatctggc gagccaggtt gatgatgtgc tctttgtcgt
     9301 aagagatcag cggacgcagg atcagcgtat cggagacgtt atcaatcagg cgcaggttgg
     9361 tcagcgtctg gctggacacc tggccgagcg cttcaccggt aaccagcgcc tgtacgccgt
     9421 aacgttctgc cactttggac gcggcacgca ccatcatacg tttgaggata acgcccatct
     9481 gaccgtcgtc gattttctcg agaatttcac cgacgaccgg ctcaaagtta atggcgacaa
     9541 aacgcacgcg gtgggagcta ccaaaacggt tccacagata atgcgccacc tgacgaacac
     9601 caatttcatg cgccgcgccg ccgagattaa agaagcagta atgcacgcgg cagccgcgac
     9661 gcatcaacat ataactggaa acaccggagt cgaaaccacc ggaaatgagc gacaacacat
     9721 cttcctgggt gccgatcggg aaaccgccaa taccttcgta gcggccttta atcagcagca
     9781 gacgatcgtc ttccacttcc agatggacag tcacttccgg attggtcagc ttcacgcgcg
     9841 cggattcaat atgctgattt aaaccgccgc cgacgtaacg ttccacatca atcgagctaa
     9901 aatcatgttt gccgcggcgc ttcacgcgta cgcagaaggt tttgccttcc agctgatcgc
     9961 gatattgaac caacgctttc tcgaaaatat cgtgcatgtc ggtaaacggc acgtcttcga
    10021 cttcgagaat atggtggata cccggaatac gggtcagggc gtcgcgaata gccagacgct
    10081 ggttttcatc ttttgcgcga acttcgatgt tatcccagtg gcggacgaca gcgagcgtct
    10141 catcatagtg ctttaaaacg ttacgaatgt tcccggtaag gatttttata aagcgcaagc
    10201 gcacagattg gcttttgatg gtgatttccg ggaacaattt aatgataaac ttcatggcgg
    10261 caatggttcg ttggcaagcc ttaagcgact tgtataggga aaaatacagc agcccacacc
    10321 tgcggctgca tccaggcgcg gaagtatacc actaacatcg ctttgctgcg cacatcacct
    10381 taccattgcg cgttatttgc tatttgccct gagtccgtta ccatgacggg gcgaaaaata
    10441 ttgagagtca gacattcatt atgccgaaga aaaatgaggc tcccgccagc tttgaaaagg
    10501 cgctgagcga gctggaacag attgtaaccc gtctggaaag tggcgacctg ccgctggaag
    10561 aggcgctgaa cgagttcgaa cgcggcgtgc agctggcacg tcaggggcag gctaaattac
    10621 aacaagccga acagcgcgta caaattctgc tgtctgacaa tgaagacgcc tctctaaccc
    10681 cttttacacc ggacaatgag taatggactt tccgcagcaa ctcgaagcct gcgttaagca
    10741 ggccaaccag gcgctgagcc gttttatcgc cccactgccc tttcagaaca ctcccgtggt
    10801 cgaaaccatg cagtatggcg cattattagg tggtaagcgc ctgcgacctt tcctggttta
    10861 tgccactggt catatgttcg gcgttagcac aaacacactg gacgcacccg ctgccgccgt
    10921 agagtgtatc cacgcttact cattaattca tgatgattta ccggcgatgg atgatgacga
    10981 tctgcgtcgc ggtttgccga cctgccatgt gaagtttggc gaagcaaacg cgattctcgc
    11041 tggtgacgct ttacaaacgc tggcgttctc gattctaagc gatgccgata tgccggaagt
    11101 gtcagatcgc gacagaattt cgatgatttc tgagttggcg agcgccagcg gtattgccgg
    11161 aatgtgcggt ggtcaggcat tagatttaga cgctgaaggc aaacacgtac ctctggacgc
    11221 gcttgagcgt attcatcgtc ataaaaccgg cgcattgatt cgcgccgccg ttcgccttgg
    11281 tgcattaagc gccggagata aaggacgtcg tgctctgcca gtactcgaca agtacgcaga
    11341 gagcatcggc cttgccttcc aggttcagga tgacatcctg gatgtggtgg gagatactgc
    11401 aacgttggga aaacgccagg gtgccgacca gcaacttggt aaaagtacct accctgcact
    11461 tctgggtctt gagcaagccc ggaagaaagc ccgggatctg atcgacgatg cccgtcagtc
    11521 gctgaaacaa ctgactgaac agtcattcga tacctcggca ctggaagcgc tagcggacta
    11581 catcatccag cgtaataaat aaacaataag tattaatagg cccctgatga gttttgatat
    11641 tgccaaatac ccgaccctgg cactggtcga ctccacccag gagttacgac tgttgccgaa
    11701 agagagttta ccgaaactct gcgacgaact gcgccgctat ttactcgaca gcgtgagccg
    11761 ttccagcggg cacttcgcct ccgggctggg cacggtcgaa ctgaccgtgg cgctgcacta
    11821 tgtctacaat accccgtttg accagttaat ctgggatgtg gggcatcagg cttatccgca
    11881 taaaattttg accgggcgcc gcgacaaaat cggcaccatc cgtcagaaag gtggcctgca
    11941 cccgttcccg tggcgcggcg aaagcgaata tgacgtatta agcgtcgggc attcatcaac
    12001 ctccatcagt gccggaattg gtattgcggt tgctgccgag aaagaaggca aaaatcgccg
    12061 caccgtctgt gtcattggcg atggcgcgat tactgctggc atggcgtttg aagcgatgaa
    12121 tcacgcgggc gatatccgtc ctgatatgct ggtggttctc aacgacaatg aaatgtcgat
    12181 ttccgaaaat gttggcgcgc tcaacaacca tctggcgcag ctgctttccg gtaagcttta
    12241 ctcttcactg cgcgaaggcg gaaaaaaagt tttctctggc gtgccaccca ttaaagagct
    12301 gctcaaacgt accgaagaac atattaaagg catggtagtg cctggcacgt tgtttgaaga
    12361 gctgggcttt aactacatcg gcccggtgga cggtcacgat gtgctggggc ttatcaccac
    12421 gcttaagaac atgcgcgacc tgaaaggccc gcagttcctg catatcatga ccaaaaaagg
    12481 tcgtggttat gaaccggcag aaaaagaccc aatcaccttc cacgccgtgc ctaaatttga
    12541 tccctccagc ggttgtctgc cgaaaagtag cggcggttta ccaagctatt caaaaatctt
    12601 tggcgactgg ttgtgcgaaa ccgcagcgaa agataacaag ctgatggcga ttactccggc
    12661 gatgcgtgaa ggttccggca tggtcgagtt ttcacgtaaa ttcccggatc gctactttga
    12721 cgtggcaatt gccgagcaac acgcagtaac ctttgctgcg ggtctggcga ttggaggcta
    12781 caaacccatt gtcgcgatct actccacctt cctgcaacgc gcctatgatc aggtgctgca
    12841 tgacgtggcg attcaaaagc ttccggtcct gttcgccatc gaccgtgcgg gcattgttgg
    12901 cgctgacggt caaacccacc agggcgcttt tgatctctct tacctgcgct gcataccgga
    12961 aatggtcatt atgaccccga gcgatgaaaa cgaatgtcgc cagatgctct ataccggcta
    13021 tcactataac gatggcccgt ccgcggtgcg ctacccgcgc ggcaacgcag ttggcgtgga
    13081 actgacgcca ctggaaaaac tgccaattgg caaaggcatt gtgaagcgtc gtggtgagaa
    13141 actggcgatc cttaactttg gtacgctgat gccagaagcg gcgaaagtcg ctgaatcgct
    13201 gaacgctacg ctggtcgata tgcgtttcgt gaaaccgctt gatgaagcgt taattctgga
    13261 aatggccgcc agccatgaag cgctggttac cgtagaagaa aacgccatta tgggcggtgc
    13321 aggcagcggc gtgaacgaag tgttgatggc ccatcgtaaa ccagtacccg tgctgaacat
    13381 tggcctgccg gacttcttta ttccacaagg aactcaggaa gaaatgcgcg ccgaactcgg
    13441 cctcgatgcc accggtatgg aagccaaaat caaagcctgg ctggcataat ccctactcca
    13501 ctcctgctat gcttaagaaa ttattcatgg caggagtggc agcatgcaat acaacccctt
    13561 aggaaaaacc gaccttcgcg tttcccgact ttgcctcgga tgtatgacct ttggcgagcc
    13621 agatcgcggt aatcacgcat ggacactgcc ggaagagagc agccgtccca tcattaaacg
    13681 tgcgctggaa ggcggcataa atttctttga taccgccaat agctattccg atggcagcag
    13741 cgaagagatc gtcggtcgcg cactgcggga tttcgcccgt cgtgaagacg tggtcgttgc
    13801 gaccaaagtg ttccatcgcg ttggtgattt acctgaagga ttatcccgtg cacaaatttt
    13861 gcgctctatc gacgacagcc tgcgccgtct cggcatggat tatgtcgata tcctgcaaat
    13921 tcatcgctgg gattacaaca cgccgatcga agagacgctg gaagccctga acgacgtggt
    13981 aaaagtcggg aaagcgcgtt atatcggcgc gtcatccatg cacgcttcgc agtttgctca
    14041 agcactggaa ctacaaaaac agcacggctg ggcgcagttt gtcagtatgc aggatcacta
    14101 caatctgatt tatcgtgaag aagagcgcga gatgctgccg ctgtgttatc aggaaggcgt
    14161 ggcggtgatt ccatggagcc cgctggcgcg gggccgtctg acgcgtcctt ggggagaaac
    14221 caccgcacgg ctggtgtcgg atgaagtggg gaaaaatctc taccaagaaa gcgatgaaaa
    14281 tgacgcacaa attgcagaac gcttaacggg cgtcagtgaa gaacttggtg caacgcgagc
    14341 acaagttgcg ctggcctggt tgttgagtaa accgggcatt gccgcaccga ttatcggaac
    14401 ttctcgggag gaacagcttg atgaactgct aaacgcggtg gatatcacct tgaagccgga
    14461 acaaatcgcc gagctggaaa cgccgtataa accgcatccg gtcgtaggat ttaaataggt
    14521 atacaggcct gatgagacgt gacaagcgtc acatcaggca tcggtgcaca actacgacag
    14581 aatacccagc ggccagtgat gtccaataaa gtacaggatg actgcggaga tcaccccggc
    14641 gacaatatcg tcaatcatga tccccatgcc gccatgtaca ttgcgatcaa accagcggat
    14701 cggccacggc ttccacatat ccagaatacg gaaaatcaca aatccggcgg taacccactg
    14761 ccagtcattg gtcggcagcg ccatgagcgt aatccacata ccaataaatt cgtcccagac
    14821 aatactgccg tgatcgtgca cgcccatgtc tttcgccgtt tgatgacaaa gatagacgcc
    14881 gatacagatc cccagcatca ccaccagcga atagagctgc cagggcaaaa aagtcatcag
    14941 ataccagaag ggaatcgccg ccagcgaccc catcgtgcca ggaacgatcg ggcttaatcc
    15001 acttccgaat ccgacagcaa gcagatgcca cggattactc attttcaggc gacttttcgc
    15061 gacatcttta tggcgtggca aaatggtcat atcctttcca gtctaacgtg acaggttcgc
    15121 cgtcacgaat aaaacaaagc ccttcgatat cggcggtcat ttgcccgata caggtaaacg
    15181 gtacgcccag gtgtccgaga gccacatcca gcgcgccacg gttcagttcc ggcaccgtaa
    15241 aacacaactc gtaatcttcg ccgccagaga gcgcccagcg cagcgcctgt tccggttcaa
    15301 catggcgaga aagcgcatca gaaaacggca gcaatgccag gtcaatacgt gcgccgcagt
    15361 cgctggcttt cacgatatgc ccgagatcgg aaatcagacc gtcagagaga tcgatagctg
    15421 aatttgccag atcgcgcagt gcctgcccct gtaaaatacg tggcgacgga cggagatgac
    15481 gtttgatcaa gtagtccgca tctttagcat cggcaacctg caaacggttt tgcaaaatcg
    15541 ccagcccggc agcgctatcg cccggtgtac cggtcacata gatccagtca cccggtttcg
    15601 ccccagagcg cgttaaggct cgtcccatcg gaacaaagcc gtggataccc aacgtcattg
    15661 ataatggccc acgcgtggta tcgccgccaa taagttgcat atcgtaataa ttgagaagat
    15721 caaacaagct gtcgctgaag gactcaagcc acgcttcgtc tacgtccggt aaggttaatg
    15781 ccagcgtcag ccaggccgga tcggccccca tcgctgccag atcgcttagg ttcaccgcca
    15841 gtgctttata agccagatca gcaggatcga tatcagggag gaagtggttg cccgccacca
    15901 gcgtatcagt gctgatcgcc agggtctgtt tctcggggat attgagaaga gcgcaatcgt
    15961 cgccgatgcc cagttcgaca tcaagacgag aacttcttac acggtcaaaa taacgggcaa
    16021 tcagggagaa ctcgccacat gccatacgtt acgcctcagc agataaaaaa gaaaaggccg
    16081 gcgatcatgg aacggtcttc cgtgaatcta ccggcttgga tattactttt tgttagggcg
    16141 aatcacaggt gctgctttat cgagtacgcc gttgacgaac ttatggctgt cttctgcgcc
    16201 gaacgatttc gccagttcga tcgcttcgtt aatggccact ttgtatggca catcgctacg
    16261 tttagacagc tcgtacagcg caatgcgcag tactgctttt tctacctggc ccagttcttc
    16321 cagcagacgg gacaggtacg gcttcatcag tccgtcgagg tatgcggtgt tagtcgccac
    16381 cccggccagc agctcacgga agtacaggac gtcaacgtct tttacatcct gttcagccag
    16441 gaactggtat tcaacatcag cgatgtcgtt ctgggacaac tgccaggagt agagcgcctg
    16501 gacggcacac tcacgagcgc ggcgacgagc agcaggtttc acggatttcc ccttactaat
    16561 ttcaggcctt gatggctttc aatacattaa tcatttcaag cgcggtcagt gcagcttctg
    16621 cacctttgtt gccagctttg gtgccagcac gttcgatcgc ttgttcaatg ctttcagtgg
    16681 tcagaacacc aaaagcaacc ggaatttcgc tgtcctgggc aacatgcgcc aggccgttgc
    16741 ttgcaccacc agcgacatat tcaaagtggg cagtgccacc acgaataacc gtacccagcg
    16801 caatcaccgc gtcgtattta ccggttttag ccagtgcacc cgccgccagc ggcagctcat
    16861 aggcaccagg cacccaaaca acggtaatgt tttcatcttt tacctgaccg atacgtttca
    16921 gtgcgtcaat tgcaccttcc agcaggctgt cattgataaa gttgttgaaa cgcgcgatgg
    16981 tgatggcgac gcgagcgtcc ggggtagcaa cgttagcttc aataatgttc attttcttcc
    17041 ttcgggttcg agtatggccc cgcagggggg cggattttag cataatattt cgtgcgctgc
    17101 ttccctttcg agccgggaga tcatgcaccc actaaatgca ggcaaacatc cgggcctaca
    17161 tgacgtatct ctttgaattt aaattgggga gcgtcggcta atttctcaag ccctggcagc
    17221 gtgcataatc cacgggcgtc gttgcctaat agtttaggtg cgatatagac aatcagctca
    17281 tcgactaaac ccgcctgtag caatgcgcca gcgagcgttg gccccgcttc cacccagatg
    17341 ctgttaatct gctgtttacc cagttgcatc attagtacaa ccagatccag gtgaccttta
    17401 tgctctggaa tcagcaaggt acgcaccgtt tccggccact cacgagaatc ttcctgcgta
    17461 cgcgcgaacc aggtttcgcc gggctgctgc acaatgcgat gttccggcgt cacgcgattt
    17521 tggctatcaa tcacaatacg tactggctga cggagatttt gttgcgggta gagcccctga
    17581 gtttgttcat ccagttcaga ccaacgcact gttaaggctg gatcatccgc cagcaccgtg
    17641 gcgctgctgg ttaaaatggc atgactttgc gcgcgcagtc gttgtacgtc gcgtcgcgcc
    17701 tgaggcgaag tgatccactg gctttcgcca ctcgccatcg ccgtgcgacc atcaagcgat
    17761 gcgccaagtt ttaactgaat ataaggaaag ccggtgcgca tccgcttgag aaagccttta
    17821 ttcaattgct cagcttcact catcatcagc ccgtggctga cgtcaatgcc agcctgttgc
    17881 agacggtaaa gtccacgtcc agcaacctgc ggattggggt cttgcattgc agcaaccacg
    17941 cgcgccacgc ccgcagcaat taatgcatca caacacggtg gcgtacgacc atgatggcta
    18001 cagggttcga gtgtgacata cgccgttgcg cctttggctt tttcgccggc catgcgcaac
    18061 gcgtgtacct cggcatgcgg ttcacccgct cgttgatggt aaccttcacc gacaatttcg
    18121 ccatctttga caatgacgca cccgacattc gggttgggat gcgtggtaaa acgtcctcgt
    18181 tgcgccagct ttagcgcccg cgccatgtaa tactcgtcct gcacggctta gtcctccagg
    18241 cgcgcgatct cttcgccaaa ttctttgata tcttcgaaac tgcggtagac agaggcaaaa
    18301 cggatatagg cgactttatc gagctttttc aattgctcca tcaccagatt gccaatcatc
    18361 ttgctcggca cttcgcgctc accggtggcg cgcagctgcg attttatatg attgattgcc
    18421 atttcgacgt catcggaact caccggacgt ttttccagcg cccgcagcat tccgctacgt
    18481 aatttctctt cattaaacgg ttcacgcacg tcgttgcttt ttacaacacg cggcataacc
    18541 agctccgcca cttcaaaggt ggtgaaacgt tcattacaca ccagacactg ccgacggcgg
    18601 cgtacggatg aaccctcgcc cacgagacga gagtcaatta ccttagtgtc cacggcgaaa
    18661 cagaatgggc aatgcatacg gcgtcctgac caggtggtta acagaaatct attttaccct
    18721 gaactgacga tacaacaaag gcgcaacgct tttgagataa aggatcgatg ctttcactgc
    18781 ttttaccctc taccattagg ctaattgcga cgagaaaggg acgacctatg aatactaacg
    18841 tttttcgact gctcctgctg ggaagcctgt tcagccttag cgcctgtgtg cagcaaagtg
    18901 aagtgcgaca gatgaaacac agcgtcagca cgctgaacca ggagatgacg caactcaata
    18961 aagaaacggt caaaatcact cagcaaaaca agctgaatgc aaaatccagc agtggggttt
    19021 accttctgcc tggagcaaaa acaccagcaa gactggaaag ccagatcggt actttacgta
    19081 tgtcgctggt gaatattacg cctgacacag atggcactac cctgacgcta cgtattcagg
    19141 gcgaatccaa tgacccttta cccgcattca gcggaaccgt tgaatatggg caaattcagg
    19201 gaacaataga caactttcag gaaatcaatg tgcagaatca attgattaac gcccctgcca
    19261 gcgtcctcgc ccccagcgat gttgatattc cgttacagtt aaagggtatt tctgtggatc
    19321 agttaggctt tgtacgtatc cacgacattc aacctgttat gcagtaaacg tattgcgggg
    19381 cgatattgtg cgtcccgcaa catctttctc cgtcattttg ttactccgct tacatcacct
    19441 ggattgatag taaaagtttg caacaagggt gaaagtcagt acaatccccg cccgaatgtg
    19501 tgtaaacgtg aacgcaatcg attacgtaaa tgatagaact gtgaaacgaa acatattttt
    19561 gtgagcaatg atttttataa taggctcctc tgtatacgaa atatttagaa acgcaatttg
    19621 cgcctttttc actcccgcaa gggattttca aacagtggca tacatatgaa aaaaacatta
    19681 ctggcagccg gtgcggtact ggcgctctct tcgtctttta ctgtcaacgc agctgaaaac
    19741 gacaaaccgc agtatctttc cgactggtgg caccagagcg ttaacgttgt cggaagctat
    19801 cacacccgtt tcggaccgca gctccgcaac gatacctacc ttgagtacga agcattcgct
    19861 aaaaaagact ggttcgactt ctatggttat gcggatgcgc cggtattctt cggcggtaac
    19921 tccgatgcaa aaggtatctg gaaccacggt tctccgctgt ttatggaaat cgaaccgcgt
    19981 ttctccattg acaagctgac caatactgac cttagcttcg gtccgttcaa agagtggtac
    20041 ttcgcgaaca actacattta cgacatgggt cgtaataaag atggccgcca gagcacctgg
    20101 tacatgggtc tgggtaccga catcgatact ggtctgccga tgagcctgtc catgaacgtc
    20161 tatgcgaaat accagtggca gaactacggc gcagcgaacg aaaacgagtg ggacggttac
    20221 cgtttcaaag tgaagtactt tgtgccgatt accgatctgt ggggcggcca gctgagctac
    20281 atcggcttca ccaacttcga ctggggttcc gacctgggcg atgacagcgg ttatgcgaac
    20341 aacggtatta aaacacgtac caataactct attgcgtcca gccatattct ggcgctgaat
    20401 tacgatcact ggcactactc tgtcgttgcc cgttactggc ataacggtgg tcagtggaat
    20461 gacgatgcag aactgaactt cggcaacggc aacttcaacg ttcgctctac cggctggggt
    20521 ggttatctgg tagtaggtta caacttctaa ttgtgagaat gccggatttc atatccggca
    20581 ttttttatca cttcttcttg ttcatcatcg ctttcaaatc agcgaatggg ttgtacttcg
    20641 cttcaccgac atctttctgc gcatcttccc ctgcgataac ggtcgtacca tactgatccg
    20701 cttcggtgta tttcgaatgc tcatgatcgt ggcaatagag acaaagtaat tcccagttac
    20761 tgccatcttc cgggttatta gtatggtcgt gatcaatgtg gtgaacggta agttcacgca
    20821 ggttggaata aacaaactcg cgggaacagc gaccgcagac ccacggatag atttttaatg
    20881 ctttttcgcg atagccgctt tctaaccgcg catagttttt tgggatgata gccatgaaaa
    20941 agttacctgg atgaaatgta gggttacgcg cagtttccca agatgcgggg attttcgcaa
    21001 tcaccttaca tcaaaaaaaa tcccgatctt ctgaccggga ttttcaacat cagctgaaac
    21061 ttgattaagg cagaattgac ggctgatccg ccccttcttt ttccactttc tgctgcaaca
    21121 tgtgttcgcg cttcataccc agtttcagag ccagcgcaga tgccacatag atggaagatg
    21181 cagtaccgat ggaaacaccg ataagcatgg tcagcgagaa gccttccagt accggaccac
    21241 cgaagaggta cagcatcagg ataaccatca aggtagtacc ggatgtgatc aaggtacggt
    21301 gcagcgtctg ggtcaaggac acgttaaaga tttcgtaagg cgtaccgcga cggatcttgc
    21361 ggaagttttc acgaatacgg tccgatacca cgatactgtc gttaagcgag taaccgataa
    21421 ccgacatcaa cgatgccaca atggtcaggt caatctcgat atggaataac gacaaaatac
    21481 ccagcgtaat gataacgtcg tgcgccagcg caataacaac ccctgccgcc agtcgccact
    21541 caaagcggaa accaacgtac acgaggatag acagcagcgc cgccatcaac gccatcgcac
    21601 cggtttgcgc aaggtctgcc cccacgctcg gaccgacgaa ctcaatacgc ttcactgccg
    21661 cattctgatt ggtggattcg ttaatcacct tcagaacctg gctgcccagc acctgaccac
    21721 cggtttcgcc ttcagcaggc ggcatacgga ccatgatgtc gtggctgcta ccaaagtttt
    21781 gcagcatcgg ctcttcaaaa ccggcttttt gcaatgcatc acgcattacg tcaatttcag
    21841 ccggtttttc gagcgtaatt tcaataaccg taccaccggt gaaatccagc ccccagttaa
    21901 agccgcgcac gcccataata acgatagcgg cgattaacag cagaccagag atgccgaaag
    21961 cccagtagtc ccagcgcata aagtcataga ctttacggcc gtggtttagt tgttcaacag
    22021 tatattcctg tgccacatcg cactcctcag attgacagct tcttgacgcg cttgccgcca
    22081 tatagcaggt ttacgatggc acgcgtaccg acaatcgcgg taaacatcga cgtcgccaca
    22141 ccgataccgg tagtaatcgc gaacccttta attgccccgg tacccactgc gtacaggatg
    22201 atgactttaa tcagcgtggt gatgttcgca tcgaagatgg aactgaatgc gccacgataa
    22261 ccttcatcaa ttgcctgttg aacagtacgt ccgttgctca actcttcttt aatacgttcg
    22321 ttgatcagta cgttcgcatc gaccgctacc gcaagggtta agacgatacc cgcgatacct
    22381 ggcatactca gcgttgcgcc tggcaacagc gacataatac cgacaatcaa gatcaagttg
    22441 gcaatcagag cactggtcgc aatcagacca aacttcttat agaagatgat catgaacaga
    22501 atagaaacca gcagaccggc aaggcaagcc tccagcccct gctcaatgtt ctgcataccc
    22561 agggttgggc caatggtacg ttcttcaaca atctgaatcg gcgcgatcaa cgcaccggca
    22621 cgcagcagca gtgaaagctg acgcgcttcg cttgggttat tgatgccagt aatacggaag
    22681 ctgttaccca gacgagactg gatgttggcg atattaatca cctcttcctg tttcaccaga
    22741 accgcacgac cgtttgcatc tttcttaccg ctgtctttgt actccacaaa cagggttgcc
    22801 atcggtttgc cgatgttgtc cttggtgaag ttagacatga tgttaccacc cgcgctatcg
    22861 agagagatgt taacctgcgg ctggttgtat tcgtcctggc tggaagtgga gtcggtgata
    22921 tggtcaccgg tcagaattac gcgtttgtac agcacaaccg gctgaccttc gcgggtctgt
    22981 ttcacttcag agtcgcccgg tacgcgaccg gatgccgcag cggcctgatc aacgttggtg
    23041 tttaccagac ggaattccag cgttgccgtc gcacccagaa tctctttcgc acgcgcagtg
    23101 tcctgaatac ctggcagttc aacaacgata cggtcagcac cctgacgctg aaccaccggc
    23161 tccgccacgc caagttggtt tacacggtta cgcaggatat tgatgttctg ctgcaccgca
    23221 tattcacgcg cttcactcag acgagcatcg ctcattaccg cacgcagctg gttgctgccc
    23281 tggctgctaa tcaccaggtc cggatggcgc ttgctcagat acgcaatggc ttcatcacga
    23341 gctttagcat cgcggaaagt gatgctcagg ccgtagttgt tttctttacg aacggtggta
    23401 tacgggatgc ccttttcacg caggtcgctg cgcaggctat cgatattttg ttcctggagt
    23461 ttgccaagcg cggtgtccat atccacttcc atcaggaagt gaacgccgcc acgcaggtca
    23521 aggccgagct tcatcggctc agcatgaata gctgccagcc agcgcggcgt tgccggggca
    23581 agattaagcg ccacgacgta tttgtcaccc ataacgccca ttaatgcttc acgagcgcgc
    23641 agctgggtgt cagtggagtc gaagcgcgca agaatagcgc cctcttccag tgccacagac
    23701 ttagcagtta ttttttcttc ttgtaacgtt ttctggacct ggatcagcgt ttgctcactg
    23761 gcggcgactc cgcgcgcacc agtgatctga acagccggat cctcaccaaa caggttggga
    23821 agcgcataca gcagaccgat gacaatcacc acgatcagca tgacgtactt ccacaaagga
    23881 taacggttta acacggcaat tcccttaggg aaaaatttta attacagcgc cttcatggtg
    23941 cctttcggca gaacggcagc tacgaagtca cgtttaataa ctacttcagt ggtgtcattc
    24001 agtgcgatag caatgtagcc gttttccgct actttggtta cgcgaccgac caggccaccg
    24061 ttcgtcagca cttcatcacc tttagcgatg gagtccatca gctttttgtg ttctttggtg
    24121 cgcttctgct gtggacgcag gatcatgaaa tagaaaatca gaccgaatac caccagcatc
    24181 aaaatcaaag acatcgggct accttgcgcc ggtgcacccg ttgccgctac cgcatcagaa
    24241 ataaaaaagc tcattaaatt tccctcatta ttaatattaa tcaacgttca aaggtggtac
    24301 ttctcgcccc tgacgctggt aaaaatcagt tacgaagctc tctaatttac cctcttcaat
    24361 agccttgcgt aaacccgcca tcaaacgctg gtagtaacga aggttatgaa tggtgttgag
    24421 tcgcgcgcct aatatttcgt tgcaacggtc gagatgatgc aagtaagcgc gtgaataatt
    24481 gcgacaggtg tagcaatcac actcaggatc gagtgggcca gtatcgctct tatacttcgc
    24541 attgcggatt ttcaccacgc catcggtcac gaacaaatga ccatttcggg cgttgcgggt
    24601 tggcattacg cagtcaaaca tatcgatacc gcgacgtacg ccctcaacca ggtcttctgg
    24661 tttaccaacg cccatcaggt aacgcggttt gtctgccgga atttgcgggc atacatgctc
    24721 cagaatgcgg tgcatatctg ctttcggctc acccacagcc agaccgccga cagcgtagcc
    24781 atcaaaaccg atatctacca gacctttaac agaaatatca cgtaaatctt cgtaaacact
    24841 gccctgaatg ataccaaaca gcgcattttt gtttccgaga ctgtcaaaac gctcacggct
    24901 acgcttcgcc caacgcagag acatctccat ggagcgtttt gcataatccc agtcagcagg
    24961 atacggcgta cactcatcaa agatcatgac gatatccgaa ccaagatcgt actgaatctc
    25021 catcgacttt tccgggtcga ggaaaatcgg gtcgccgttg atcgggttac ggaagtgaac
    25081 accctgttcg gtgattttac gaatatcacc aaggctgaaa acctggaagc cgccggagtc
    25141 ggtaaggatt ggtcctttcc actgcataaa atcgtgcaga tcgccgtgca gtttcatgat
    25201 ttcctggccc gggcgcagcc acaggtggaa ggtgttgccg aggataattt gcgcgccagt
    25261 ggcttcaact tcttccggcg tcatcccttt tacggtgccg taagtgccaa caggcataaa
    25321 acaaggcgtt tccactacgc cacgatcaaa gaccaggcgg ccacggcgtg cgcgaccgtc
    25381 ggtggtgtcc agttcaaatt tcattttttc tccactacgt cagaaaaaca gtccaacgtt
    25441 taaaccagcg ccgcggaatt actccccaac gcgctcatta attgcctgcg gattgtacgt
    25501 gataaacatc gcatcaccgt aactaaaaaa gcgatatttc tcttctaccg ccgctttata
    25561 ggcattcatg gtgtgttgat aaccagcaaa tgccgaaacc agcataatca gcgtcgattc
    25621 cggaaggtgg aagttcgtca ccagcgcatc gaccactttg tactggaagc ccggatagat
    25681 aaagatttgg gtatcgtcaa agaacggttc aatgagatcg tttttcgctg cctgagccgc
    25741 actttccagc gaacgtactg aggtggtgcc aaccgcaatc acccggttac cgcgcgcttt
    25801 cgccgccagt accgcgtcta ccacatcctg cggcacttca gcgtattccg agtgcatgat
    25861 gtgatcttca atggtgtcga cgcgcaccgg ctggaaggtg cccgcaccaa cgtgcaacgt
    25921 cacaaacgcc atctccacgc ctttggcgcg taatttttcc agcaaaggtt cgtcaaagtg
    25981 cagtccggcg gtcggtgctg ctaccgcgcc gggtttttcg ctataaaccg tttgataaag
    26041 ttcgcggtct gcatcttcgt ccggacggtc gatgtacggc ggcagcggca tatggccgat
    26101 gctgttgaga atatccagca ccgaacgttg atcattaaat tcgacttcaa acagtgcgcc
    26161 gtggcgcgcg gtcattgttg cgttaatact ttcgtcatcg cccagcagca gttctgcgcc
    26221 aggttttggc gctttcgagg cgcgaatatg cgcaagaatg cgtttgtcgt cgagcatccg
    26281 ctcaaccagc acttcaatct tgccgccgct ggctttacgc ccaaacagac gcgccgggat
    26341 cacgcgggta ttattaaaaa ccagaagatc gccggggttg agcttatcga gtaaatcggt
    26401 gaaagtaccg tgcgtcagcg cgcccgtcgg cccgtccagc gacagtaaac gacagctact
    26461 gcgttcaggc atgggatagt gggcaatcag ggattcgggc aattcaaaag agaaatcggt
    26521 aacgcgcatg acactgactc agactaaaat aagaggcggg tagtctagtg ccggggcgct
    26581 ctccctgcaa cctttacccc cctttggata gaatataatc gatgaatttt ttagctcacc
    26641 tgcatttagc ccatctcgcg gaaagctcgc tttccggcaa tttactggct gatttcgtac
    26701 gcggaaatcc tgaagaaagt tttccgcccg acgtcgtggc tggcattcat atgcatcggc
    26761 gaatcgacgt attaaccgac aatctgccag aagtccgcga agcgcgggag tggtttcgta
    26821 atgaaacgcg ccgtgtcgcg cctattacgc tggatgtgat gtgggatcac tttctgtccc
    26881 gccactggtc gcagctgtcg cccgattttc cgctacagga atttacctgt tatgcccggg
    26941 agcaagtgat gactattctt ccagactcac cgccacgctt tatcaatctg aacaactact
    27001 tatggtcaga gcagtggttg gtgcgctatc gcgatatgga tttcatccag agcgtgttaa
    27061 acggcatggc aagccgtcgc ccacgtctgg atgccctgcg tgactcctgg tacgatttag
    27121 acgcccatta cgatgctctc gaaacccgct tctggcagtt ttatccgcgg atgatggagc
    27181 aggcgtcacg caaggcgtta taagacgtta attcatccac accgtagccg aaatggcagg
    27241 caaagccaga atcccgtccg tcagttgccc gtgaccttct ttgcgttgcc attgcgcgac
    27301 attaagcaac ggcgacgcgg gtagcaccac ttcacaggct tcgccacggt tgattgccac
    27361 cagtacacgc tgctgattca gcacgcggac aaataccacc acgttatctt ccgcatacag
    27421 cacctgacag ccgccacgac gcaacgcctg acttttctta cgcagcgcaa tcattcgctg
    27481 gtacagcgcg aataacgccg aatcctgctt ttccacctgc caggggaacg gcttacggca
    27541 aaacggatcg tttttgccat ccagtcccac ttcatcaccg taataaatgc acggtacacc
    27601 aggccaggtg aacagccaga ccaccgccag gggcaggcgc gcaacatccc gaccgagcag
    27661 cgttttaaat cgcgcagtat cgtggctgtc gagctgatta aacatacgta attgttgttg
    27721 atgagaaagc cctgcgcggt aattatccat ccaggccata caggtttggg cgtcaatttg
    27781 ctgcggatcg taagagatat cggtgttggc aagaaatccc cacaacggga atgtgaagcc
    27841 gcgatagttc atggcggcat cttccacatc ggcctgtaac cattgccgtg catcgccaaa
    27901 atgttcgccg acaatatacg catccggctg ggtttctttc gccgcttcgg tgatcccggc
    27961 aacgtgctgc aaattattgc gcgccccacc cgcttccccc agcatatgca ccacatccag
    28021 ccgccagcca tccatattcc acggcgcttt cagccagtgg cggacaatac tgtcttcccc
    28081 gcgataaatt tcattcacca gactttccga ctgataatcc agcttcggca agctggcata
    28141 gcccagccag tcgagcgccg tgccatcatc actaaacgag taccagtcac gccagggtga
    28201 ttcggggttg tgacaagctc cccccgtgcc acgattgtgc ctgtcaaacc aggcatggga
    28261 atcgccactg tggttaaaca cgccgtccag caccagtcgt attcccagct gctgcgtatt
    28321 gtgtcgcaaa cgcagcaacg ccccatcacc gccaaactgc ggatcgacat ggcgataatc
    28381 ctcggtatcg tatttatgca cgctgggagc tttaaacacc ggattgagat acagcgccgt
    28441 cacgccaagt tttttcagat acggcagttt ttcacttatc ccgtccagat cgccgccata
    28501 gaacgttgat ccgccagcct gcgccgtgac cggttcatcc cagtcacgca atatgatctc
    28561 ttgtccggct gcgtgatggt aatagacatg atcctgttca gcttcacgag gaagattacg
    28621 ggcgaaacga tcagggaaga tctgataaaa aatctgatcc gcagcccatt gtgggccgat
    28681 atccggtaca tcgatggcaa actgctcctg tcgtgccggc ggcattcggc tgaagccttg
    28741 cggtgtaaac caacgctggc gatcgtgcca cagcaatttg aaactataac gccgccgagg
    28801 ttgtccgcag gagagctcga tcgccgcccg ccatgcggtg acgccaggct gcggctgact
    28861 gcgctgctta tgcatcggta ctgacatttc ttcgttatcg tgttctgtac gcagcataat
    28921 gcgctgcggt gggtcttcgc ccgtcagcca cagtgtaatg agcagttgat ctttgctttg
    28981 tttaacaaat gggggcaccg gcagatgcca tgcatttaac atcataattc ccctgtgatg
    29041 aaaatctgat caccttgcca tagcctaatg caggatcact catcatctga aggatatttg
    29101 ggggaggagg acgggggcgg ataactttcg gaatagccgg atcaggattt cctcctgaac
    29161 cggcttacag aacgcaagga ttactggttt tcagccagct gacgatcgtg gcggcgctta
    29221 aacatccagc caatcaacag cacaacaatc cacgcgaaac caacatacag cgagatacgc
    29281 gtatcagggt gataaccaat caacccgata ataaagagca ggaaaatcaa accgccgatg
    29341 gtcgttgcta ccccacccgg cactttaaat ttcagcgcct taacttcttc tggcggcaaa
    29401 cggcgacgga aggcgatttg cgacagcagg atcataatcc acacccacac cgtggcgaag
    29461 gttgccagcg aagcgatcac caggaagacg ttttccggca tgatgtagtt cagatacacc
    29521 gcaaacagca gcgcggtagt catcaccagc accgtaaccc acggaatacc gcgacgcgag
    29581 gttttgctga aaatttttgg cgcgctgccc tgctctgcca taccgtggag catacggcct
    29641 acaccaaata cgtcactgtt aattgccgac agcgaagcgg tcagcacaac aaagttaaga
    29701 atgctggcgg caaaggtaat gcccatatgc tggaacgtca gcacaaacgg gctaccggca
    29761 gtgccaacct gattccacgg gtagatagac ataatgacga acagcgtacc gacgtagaac
    29821 accagaatac gcatcggcac ggagttaatc gcacgcggaa tcgatttctc aggatctttc
    29881 gcttcaccgg cggtaatccc gataatttcg atcccaccgt aagcaaacat caccatttgc
    29941 aacgacatca ccataccaag ccagccgtta ctgaagaagc cgccgttgct ccacaggtta
    30001 tgaataccgg tcggttgccc gccgttgcca atcccccaga tgatgatgcc gaaaccggcg
    30061 acaatcatga tgatgatagt ggcgacttta aagaacgaga accagaactc cagctcaccg
    30121 aataccttca cgctcatcag gtttacggcg caaatgatca gcaccacact cagtacccaa
    30181 atccagtgcg gcaccgtcgg gaaccagaca cccatataga taccaaaagc ggtcacatcg
    30241 gcgatggcga caataaggat ttcaaagcag taggtccagc cggtaatgta acctgccagc
    30301 gggccgaggt tttcctgcgc ataacgcgag aaagagctgg ctgccgggtt atgtaccgac
    30361 atttccccca gcgcacgcat aatgatatac gccgcgatac caccgataat ataggccaac
    30421 aacacgcttg gaccggccat tttgatggca tctgccgaac cgtaaaacag cccggtgcca
    30481 attgctgaac ccagtgccat aaagcgtatg tgtcgggtac ttagcccacg ctttagcttg
    30541 ttcttacttt ccatcaattc ctaacccatc aacacaataa aaaaccacgg ggcatcgcag
    30601 ccccgtggtt aaaacaaatg ttcagtgatt tagtgagcgc tggaggtcac ctgacgacct
    30661 gccgcgcgat cccagataat ggccagaacc accatcacca ctgttggcat taaccacgcc
    30721 agaccttgtt cggccagcgg taaacgctgc gcccaggacg gtaagatatc gctgaatgca
    30781 gatgccttga tcccgtcgag aataccaaaa agcaggctga taaacatcgg cggagcaatc
    30841 acgcgggacg aattatgcca ccatgagcgt gtaaaactta atacaaccag tgcgatacac
    30901 ggcggataaa tggcggtcag caccggtaca gagatctgaa tcagctggct caagccgagg
    30961 ttagacacca ccatcgagaa gccgccgagg ataaacacca gcgtacgata agagagcggt
    31021 acgtactggg cgaagaattc tgcacaagca caggtcaggc caaccgccgt gaccaggcag
    31081 gcgatgaaga ttaacgccgc cagcaggaag ctaccgccgc cgccaaaggt atgctgaacg
    31141 taagcatgca ggatcgccgc accgtttgca gactgatcga ccagcgacgc gctgtctgaa
    31201 cccagacgga acagcgccag gtacagcaga gtcagaccaa cacccgccat caggccagcc
    31261 cagacggtat aacgggtcag cagacgcgct tcggtaacgc cacgagaacg cgccgcgtta
    31321 acaataacga taccaaacac cattgcgccc agcgtatcca tggtcagata gccgttgacg
    31381 aagccgttag aaaacgcagc gttttgataa gcctcagtcg ccgtgctgat agaacccgcc
    31441 ggccagataa ttgcggcaac agacaggatg accagcgcga taattttcag cggcgcaagg
    31501 aagttgccca cggtatccag cagcttgccc ggatagagcg aaaccagaat aacgatagcg
    31561 aaatagacca ggctgtaaat aaacagcggc agcgcggaat cacccgtcag cggcgcaatg
    31621 cccacttcaa aagaaacggt agctgtacgc ggcgtagcga aaagcggccc caccgccagg
    31681 taacaaactg ttgccagcag tacgccagcg actttaccaa ttggcgtgct gagactgtca
    31741 acaccgccgc caacttttgc cagcgccact accgttaata ccggtaggcc aacggcagta
    31801 atgaggaagc cgaatgccgc agtccagacg tgttcgcctg cctgcaagcc gaccattgga
    31861 gggaaaataa tgttacctgc gccgacgaac aacgcaaatg tcataaagcc cagagcgatg
    31921 atatcgcgcg atcttaattg atgggtcata aatcatactg cctgtggatg tggtgttgta
    31981 acgttaaatt tttgtgccaa tccctgggga tgatacagcg gtatatttaa acaattttag
    32041 cgggaaatac tcccaaccag gcgcggcgaa gaatagtttt tcgatttacc gcgtaaatgc
    32101 agtcagtatg acagcacctg aagcgcaatt taaacgctta taacgtttta aagcaagaag
    32161 ggatctaaaa accagaggta tatagcgata agaaatcacc aaccagtata aaacactatt
    32221 tcaatgaaaa agacatggct aatcatgcga acaaaaaatc accaaccatt gtaaaatcta
    32281 acgcccgtat ttcgactgca aagaaaataa gccagcatcg gctggcttat ggcaataaaa
    32341 gatgacaaag gcgaattaat cgctgttttt ggcaattaaa cgttccggga taataaaact
    32401 gaaacgtgta ccttttccta ctgtactctc aatattcagg cgactttcgt gatgattcac
    32461 cgcatgtttc acgatcgcta accctaatcc gctaccaccg gtttgccggg aacgcgcttt
    32521 atcaacgcga taaaaacgct cggtcaggcg cggaatatgc tccggtgcaa tgcccggtcc
    32581 gttatcttca acactaaatt cggcaccgtg cggcactcgc tgccagcgta cggtgatatg
    32641 cgtgccttcc ggcgtatgat tcacggcgtt atagaccagg ttcgaaatcg cactacgtag
    32701 ctgatcttcg ttgcctgaca ccttgaggcc gttatctatc tcaaaggtaa atgtctgttt
    32761 tttctgactc agagtctgag cctcgcgctc aacgacgcgc agcatcatcg gcacatcaac
    32821 cttttcattg agcaaatgcg tcggtgcggc ttctattttc gacagcgtca gcaattgctt
    32881 caccaaccct tccatccgct gggtctgctc acgcatggtg tgcaacgctt tttcacgtac
    32941 cgcgccttcc agcggttgct catccatcat ctccaggtaa ccctgtaaca cggtcaatgg
    33001 cgtacgtaac tcatggctca cgttggcaaa aaagttacgc cgcgcccctt ccagttgatg
    33061 catttgcgtg acatctcgcg ccaccatcag caactgtttg tgggtataag gcatgacgcg
    33121 aatctccaga tgccgcccgg tattgagcac cagattgagc gggcgagaga aatcacgcgt
    33181 tttcagatat tgcgtaaact ccgggtaacg cagcaagtta aggatgttct gcccgttatc
    33241 ttccggccag cgcaagccaa gaatctgttg cgccagaccg ttgcaccaga aaataccgcc
    33301 ctcttccgtg gtcagcacca ccgcgtcggg cagcgactcc gcgccgctac gaaagcgttt
    33361 aatcagattg cccagttcac ggcggcgttt tttatttcgc agctgcatct ggtgtaagcc
    33421 gtatagtagc ggttcccagc taccacgccc cggtggcggg gtcatactac gatccaccca
    33481 cagccaccat gaaaggcgca ataaattcca gaaatgccag ataagcagtc ctgttaccga
    33541 tgccagcaaa aaccagggca ggtaacccaa aaatgcaccc aggatgaaag ccgggaggca
    33601 gcaaaatagc agctccagca ccagcctttt ccacgacagc cgttccagca cgtaagatac
    33661 tccagttaag aaatcataag ccctgctcta cgtccgatga gcaaggcctt aaaagcgggt
    33721 tgaaaaacga taccctgtac cgcgcacggt ctgcaccatg cggtcatgcc cgccgggctc
    33781 cagtgcttta cgcaggcgac gaatgtggac atcgaccgta cgatcttcca catacacgtt
    33841 ggttccccat acgtgattta acagttgctc gcgactgtac acgcgctcgg gatgcgtcat
    33901 aaaaaagtgc agcagtttaa attcagttgg ccccatctcc agcggttctt cgcccgccat
    33961 cacccggtga gatgtcggat cgagacttag cccctgcatc tcaatcacct cttccaccgc
    34021 catcggcgat atacggcgca ttaccgcttt aattcgcgcc accagctcct tcggagaaaa
    34081 cggcttggtg atatagtcat ccgcaccggt ttcaaggccg cgcacgcgat cttcttcttc
    34141 ccctctggcg gtcaacatca ccactggaat atcccgggtc atcgactcgc gcttgaggtg
    34201 tttgatgaac tggataccgg agccgccagg taacatccag tcaaggagaa ttaaatccgg
    34261 ccagggttca ttcagttgat tcacagcact gtcataatct tccgcttcga ccggctgaaa
    34321 gccattttgt tcgagcacga agcagaccat ttcgcgaatt ggagcttcat cttctacgac
    34381 cagaatacgt ctcgccatga tttgccctgt tgtaataaat aggttgcgat cattaatgcg
    34441 acgtcattat gcgtcagatt tatgacagat ttatgaaaag ctcgtcgcac atatcttcag
    34501 gttattgatt tccgtggcac agaaaaaagc aaatggcaca tctgtttggg tataatcgcg
    34561 cccatgcttt ttcgccaggg aaccgttatg cgcatccttc acacctcaga ctggcatctc
    34621 ggccagaact tctacagtaa aagccgcgaa gctgaacatc aggcttttct tgactggctg
    34681 ctggagacag cacaagccca tctggtggat gcgattattg ttgccggtga tgttttcgat
    34741 accggctcgc cgcccagtta cgcccgcacg ttatacaacc gttttgttgt caatttacag
    34801 caaactggct gtcatctggt ggtactggca ggaaaccatg actcggtcgc cacgctgaat
    34861 gaatcgcgcg atatcatggc gttcctcaat actaccgtgg tcgccagcgc cggacatgcg
    34921 ccgcaaatat tgccccgtcg cgacgggacg ccaggcgcag tgctgtgtcc catacctttt
    34981 ttacgcccgc gtgacattat taccagccag gcggggctta acggtattga aaaacagcag
    35041 catttgctgg cggctattac tgattattat caacaacatt atgccgatgc ctgcaaactg
    35101 cgcggcgatc agcctctgcc catcatcgcc actggacatt taacgaccgt gggtgccagt
    35161 aaaagtgacg ccgtgcgtga catttatatt ggcacgctgg acgcgtttcc ggcacaaaac
    35221 tttccaccag ccgactacat cgcgctcggg catattcacc gcgcgcagct tattggcggc
    35281 atggaacatg ttcgctattg cggctccccc attccactga gttttgatga atgcggtaag
    35341 agtaaatatg tccatctggt gacattttca aacggcaaat tagagagcgt ggaaaacctg
    35401 aacgtaccgg taacgcaacc catggcggtg ctgaaaggcg atctggcgtc gattaccgca
    35461 cagctggaac agtggcgcga tgtatcacag gagccgcctg tctggctgga tatcgaaatc
    35521 actactgatg agtatctgca tgatattcag cgcaaaatcc aggcattaac cgaatcattg
    35581 cctgtcgaag tattgctggt acgccggagt cgtgaacagc gcgagcgtgt gttagccagc
    35641 caacagcgtg aaaccctcag cgaactcagc gtcgaagagg tgttcaatcg ccgtctggca
    35701 ctggaagaac tggatgaatc acagcagcaa cgtctgcagc atcttttcgc cacgacgttg
    35761 catagcctcg ccggagaaca cgaagcatga aaattctcag cctgcgcctg aaaaacctga
    35821 actcattaaa aggcgaatgg aagattgatt tcacccgcga gccgttcgcc agcaacgggc
    35881 tgtttgctat taccggccca accggtgcgg ggaaaaccac cctgctggac gccatttgtc
    35941 tggcgctgta tcacgaaact ccgcgcctct ctaacgtttc acaatcgcaa aatgatctca
    36001 tgacccgcga taccgccgaa tgtctggcgg aagtggagtt tgaagtcaaa gatgaagcgt
    36061 atcgcgcgtt ctggagccag aatcgggcgc gtaaccagcc ggacggtaat ttgcaggtgc
    36121 cacgcgtaga gctggcgcgc tgtgcagacg gcaaaattct cgccgacaaa gtgaaagata
    36181 agctggaact gacggcgacg ttaaccggac tggattacgg gcgcttcacc cgttcgatgc
    36241 tgctttcgca ggggcaattt gctgccttcc tgaatgccaa acccaaagaa cgcgcagaat
    36301 tgctcgagga gttaaccggc actgaaatct acgggcaaat ctcggcgatg gtttttgagc
    36361 agcacaaatc ggcccgcaca gagctggaga agctgcaagc gcaggccagc ggcgtcgcgt
    36421 tgctcacgcc ggaacaagtg caatcgctga cagcgagttt gcaggtactt actgacgaag
    36481 aaaaacagtt aattaccgcg cagcagcaag aacaacaatc gctaaactgg ttaacgcgtc
    36541 tggacgaatt gcagcaagaa gccagccgcc gtcaacaggc cttgcaacag gcattagccg
    36601 aagaagaaca agcgcaacct caactggcgg cgctcagtct ggcacaaccg gcacgaaatc
    36661 ttcgtccgca ctgggaacgc atcgcagaac acagcacggc gctggcgcat actcgccagc
    36721 agattgaaga agtaaatact cgcttacaga gcacaatggc gcttcgtgcg agcattcgcc
    36781 accacgcggc gaagcagtca gcagaattac agcagcagca acaaagcctg aatgcctggt
    36841 tacaggaaca cgaccgcttc cgtcagtgga acaacgaact ggcgggttgg cgtgcgcagt
    36901 tctcccaaca aaccagcgat cgcgagcatc tgcggcaatg gcagcaacag ttaacccatg
    36961 ctgagcaaaa acttaatgcg cttgcggcga tcacgttgac gttaaccgcc gatgaagttg
    37021 ctagcgccca ggcgcaacat gctgagcaac gcccactgcg tcagcgcctg gtcgcactgc
    37081 atgggcagat tgttccccaa caaaaacgtc tggcgcagtt acaggtcgct atccagaacg
    37141 tcactctgga gcagacgcaa cgtaatgccg cacttaacaa aatgcgccat cgttataaag
    37201 aaaagatgca acaacttgcc gatgtgaaaa ccatttgcga gcaggaagcg cgcattaaaa
    37261 cgctggaagc ccagcgcgca cagttacagg cgggtcagcc ttgcccactt tgtggttcca
    37321 ccagccaccc ggcggtcgag gcgtatcagg cgctggaacc gggcgttaat caggcccggc
    37381 tattaacgct ggaaaaagaa gtgaaaaagc tcggcgaaga aggtgcgacg ctacgtgggc
    37441 aactggacgc cttaacaaag caacttcagc gcgatgaaaa cgaagcgcaa agcctccgac
    37501 aagatgagca agcacttact caacaatggc aagccgtcac ggccagcctc aatatcaccc
    37561 tgcagccaca ggacgatatt caaccgtggc tggatgcaca agatgagcac gaacgccagc
    37621 tgcggttact cagccaacgg catgaattac aagggcagat tgccgcgcat aatcagcaaa
    37681 ttatccagta tcaacagcaa attgaacaac gccagcagca gctcttaacg gcattggcgg
    37741 gttatgcact gacattgcca caggaagatg aagaagagag ctggttggcg acgcgtcagc
    37801 aagaagcgca gagctggcag caacgccaga acgaattaac cgcgctgcaa aaccgtatgc
    37861 agcagctgac gccgattctg gaaacgttgc cgcaaagtga tgaactcccg cacagcgaag
    37921 aaactgtggc gctggaaaac tggcggcagg ttcatgaaca atgtctcgca ttacacagcc
    37981 agcagcagac gttacagcaa caggatgttc tggcggcgca aagtctgcaa aaagcccagg
    38041 cgcagtttga caccgcgcta caggcgagcg tctttgacga tcagcaggcg ttccttgcgg
    38101 cgctaatgga tgaacaaaca ctaacgcagc tggaacagct caagcagaat ctggaaaacc
    38161 agcgccgtca ggcgcaaacg ctggtcactc agacagcaga aacgctgaca cagcatcagc
    38221 aacaccgacc tggcgggttg tctctcactg tgacggtgga gcagattcag caagagttag
    38281 cgcaaactca ccaaaagttg cgtgaaaaca ccacgagtca aggcgagatt cgccagcagc
    38341 tgaagcagga tgcagataac cgtcagcaac aacaaacctt aatgcagcaa attgctcaaa
    38401 tgacgcagca ggttgaggac tggggatatc tgaattcgct aataggttcc aaagagggcg
    38461 ataaattccg caaatttgcc caagggctga cgctggataa tttagtccat ctcgctaatc
    38521 agcaacttac ccggctgcac gggcgctatc tgttacagcg caaagccagc gaggcgctgg
    38581 aagttgaggt tgttgatacc tggcaggctg atgcggtacg cgatacccgt accctttccg
    38641 gcggcgaaag tttcctcgtc agtctggcgc tggcactggc gctttcagat ctggtcagcc
    38701 ataaaacgcg tattgactcg ctgttccttg atgaaggttt tggcacgctg gatagcgaaa
    38761 cgctggatac cgcccttgat gcgttggatg ccctgaatgc cagtggcaaa accatcgggg
    38821 tgattagcca cgttgaagcg atgaaagagc gtattccggt gcagatcaaa gtgaagaaga
    38881 tcaatggact gggctacagc aaactggaaa gtgcgtttgc agtgaaataa ctattcagca
    38941 ggataatgaa tacagagggg cgaattatct cttggcccga ctggtcgtta tcctgcaagc
    39001 tatcacttta ttggctacgg tgattggtag ccgttctggt ggttgtgatg gtggtatgaa
    39061 aaaagttatt ttatctttgg ctctgggcac gtttggtttg gggatggccg aatttggcat
    39121 tatgggcgtg ctcacggagc tggcgcataa cgtaggaatt tcgattcctg ccgccgggca
    39181 tatgatctcg tattatgcgc tgggggtggt ggtcggtgcg ccaatcatcg cgcttttttc
    39241 cagtcgctac tcactcaaac atatcttgtt gtttctggtg gcgttgtgcg tcattggcaa
    39301 cgccatgttc acgctctctt cgtcttacct gatgctcgcc attggtcggc tggtttccgg
    39361 cttcccgcat ggtgcatttt ttggcgtcgg cgcgatcgtg ttatcaaaaa ttatcaaacc
    39421 cggcaaagtc accgccgccg tggcagggat ggtttccggg atgacagtcg ccaatttgct
    39481 gggcattcca ctgggaacgt atttaagtca ggaatttagc tggcgttaca cctttttgtt
    39541 gatcgctatt tttaatattg cggtgatggc atcggtctat ttttgggtgc cggatattcg
    39601 cgacgaggcg aaaggaaaac tacgcgaaca atttcacttt ttacgcagcc ctgccccgtg
    39661 gttaattttc gctgctacga tgtttggcaa cgcaggtgtg tttgcctggt tcagctacgt
    39721 aaagccatac atgatgttta tttccggttt ttcggaaacg gcgatgacct ttattatgat
    39781 gttagttggg ctagggatgg tgctggggaa tatgctaagt ggcaggattt caggacgtta
    39841 ttcaccactg cgcattgcag cagtgactga ctttatcatt gtactggcac tgctgatgct
    39901 ctttttcttc ggcggtatga aaacaacgtc gcttattttt gcttttattt gttgcgcggg
    39961 attatttgcc ctttcagcac ctctacaaat attattactg caaaatgcca aaggcggaga
    40021 gttattaggt gccgcaggtg ggcaaatagc gtttaacctc ggtagcgccg tcggcgcata
    40081 ttgcggtggt atgatgctga cgctggggct ggcatataat tacgtggcgc tgcccgccgc
    40141 cctgctttcg tttgctgcga tgtcgtcgtt gctgctgtat ggtcgctata agcgccagca
    40201 agcggcggat agtccggtgc tggcgaaacc actggggtag attgcagtca cggtgcacgt
    40261 tgcttattgc cggatgcggc gtgaacgcct tatccggcct acgggactgt tcattttgta
    40321 ggcctgataa gacacgccag tgtcgcatca ggcatcggag caccgggggg ttgccgccac
    40381 tgtgcgcgtt atttgttgcc ggatgcggcg cgagcgcctt atccggccta ccggacggct
    40441 cactttgtag gcctgataag acgcggcaag cgtcgcatca ggcatgatac gccaattgcc
    40501 tgggtttttt actcttgtgg ccataaccat gcagcgccgc gtacgccgct cgaatcacca
    40561 tgtttcgcct tgtgtaccgg cgtttcacat tctccgccga agacaaattg tttaatcaac
    40621 tgcggaaccg tttgatataa acggtctaca ttgctcattc cgccgcccag gacaatcaca
    40681 tccggatcga gaatattcac gacatgtgcc agcgattttg ccagccgcag ctcgtagcga
    40741 cgcaatgcca gttccgctac cggatcgctt tcttcaacca ggcggataat ttcactgcct
    40801 ttcagcgcat gtccgctcaa acgacgataa tccgtcgcga atcccgtgcc tgaaataaag
    40861 gtttcaatac aaccttgttt accgcaataa caagggactt cctcgcgata acgcagttcg
    40921 tcttcgtcca tccacggtag cggattgtgt ccccactcgc ctgccgtgcc attgccgccg
    40981 atatgcgccc gcccattgaa tgccacgccc gcgccgcatc ccgtgccgat aatcacggca
    41041 aaaaccgtct gcgctcccgc tgccgcgcca tctaccgctt ctgaaaccgc cagacagttg
    41101 gcgtcatttg ccagccgcac ttcccgctgc aaccgcgcgc ttaagtcttt atcgaatggc
    41161 tgaccgttga gccaggttga attggcattc ttcaccacgc cggtgtaggg cgaaatcgaa
    41221 cctggaatgc ccatacctac cgttccgcgc tgccctgttg cctgctccgc catatcaacc
    41281 aacgtggcga tcgtttcaat agtctgccgg taatcatcac gcggcgtggg cagacgatgg
    41341 cggtacaact gctcccctgc atcgcccaat gcaatcactt cagttttggt gccgcctaaa
    41401 tcgataccta tacgcacggt actctcctta tttttttcaa tatcaatagc gtagagacgg
    41461 acaaccggat tggcaatgca aggccgccga caattcgtta tcatgcccgc taaatttaac
    41521 gacaaggccg tggaaattat catgctgtgg ttcaaaaatt taatggttta ccgtcttagc
    41581 cgcgagattt cgctgcgtgc agaagagatg gaaaaacagc tagcctcgat ggcatttacc
    41641 ccatgcggca gccaggacat ggcgaagatg ggctgggtac ctccgatggg atcgcacagc
    41701 gatgcgttaa cgcacgttgc caatggtcaa attgttatct gtgcgcgcaa agaagaaaaa
    41761 atcctcccgt ccccggtgat taaacaggcg ctggaagcga aaatcgccaa actggaagcg
    41821 gaacaggtgc gtaagctgaa gaaaaccgaa aaagattcgc tgaaagacga agtgctgcac
    41881 tctctgctgc cgcgtgcttt cagccgtttt agccagacaa tgatgtggat cgatacggtt
    41941 aacggtttga ttatggtgga ctgcgccagc gccaaaaaag cggaagatac gctggcactg
    42001 ctgcgtaaaa gcctggggtc gttaccggtt gtaccattga gcatggaaaa cccgattgaa
    42061 ctgacgctga ccgaatgggt acgttccggc agcgcggcac agggcttcca gctgcttgat
    42121 gaagccgagc tgaaatcgtt gctggaagat ggcggcgtga tccgcgcgaa gaaacaagat
    42181 ctgaccagcg aagagatcac caaccacatt gaagccggaa aagtggtgac taaactggcg
    42241 ctcgactggc agcagcgcat tcagtttgtg atgtgcgacg atggttcgct caagcgtctg
    42301 aagttctgcg acgagctgcg cgatcaaaac gaagatatcg accgtgaaga tttcgcccag
    42361 cgctttgatg ccgatttcat cctgatgact ggtgaactgg cagcgttaat tcaaaacctg
    42421 attgaaggat taggtggcga agcacaacgt taattgctga tttaccttta atgccggatg
    42481 cgacgcctgc cgcgtcttat ccggcgtacg aagccacacc aggaatataa tcgttagcta
    42541 cagcgggcaa taattcttag cgcagcaata ttatgcgttt tatgctgtaa cttgctccag
    42601 gggcgttgtg tcattgtttt tcctcaagcc ggtgacgcgc ttcactgcga atttttcgcg
    42661 ttgccccggt tttcactaaa tcacgtaaca gcgaaagatc gggttgttcg acaaataaga
    42721 gtaatgatgg atcctctttt aaccacgccg ttttcacatc ggcagccagc tgtgaattat
    42781 taatagccag cgaccgctcc tgaggctcag tcaatgtcgt taacaacaac accggggtat
    42841 gtgcgttgtg caatgccgtt tggcgcaaat gatcattgcg actttgcagc aatgcacgcc
    42901 accattcatc atctgattta tcttttatcg tcgccagttc cgcaacaata tctggagatc
    42961 ttcgctgata ttgatatcaa ccttgatgaa ttatgaatat atcggaaatc gaaaaaatcc
    43021 cagcaaagga aatattatta tgggttaaag agaggacatt tcagaaaaat gccctcccgt
    43081 tcaaggggaa ggcgaggaat tacagatagc ggcacagata agaggtgggt tcggcaactt
    43141 gcagatgaaa ctcgctgtga ccgggaacat taaacaccga accggcttca tacacttgcc
    43201 agtcggtcgc gtcaggcagt aacacattca gcgcgccact gattaccgtc atctcttcag
    43261 gctcagcggt gctgaaggtg tattcacctt caaccataac acccacgctg gcgcgaccag
    43321 tgctgctgct ggaaaagccg atcgatttca ctttgccgga aaagtactca ttactttgaa
    43381 gcataaactg gccctgtctg gtagttgaac atctacttca ctataggggc cagaggcgct
    43441 ggctgtcacg caaaattaca tcagtaattc cgcagccagc cgtgcaatca atacgttaga
    43501 gagcaagaca ggaacatcga gctgtttttg cagtaaatcg cgatgacgct ggttaaatcc
    43561 caaacaatcc agcatgatga catctgcacc ttttgccagt aattctttcc cggcatcaat
    43621 gattttttgt tccgaatcat gaatggggtt acccaatgaa aatactggcg gtttctgcaa
    43681 aatttgccat ttttgcgcct gaacggtcag caactcctca accggtacga taacccccac
    43741 ctgatgatct tcaacaatag aggaaaccag cggtggcaat atccgcgacg gctcaagaaa
    43801 gatcgtatta cgcgcggtca tactactaat gtttgctgta ctcattaata taatgacgtc
    43861 gtaaccctga ttatcgagca cttcaaccac accttgcagg tctcgctcaa ctttgcgacg
    43921 cgaaacatgg gccagatggt tgtcatttaa taatgtgaga attgtgtctt cgcctgcttc
    43981 tggcgcgtac tccgccatca cttcttcgcg gcttaacttc cccagcaggc tatgatggga
    44041 aatattatct tcgtcaatgt attctgtcag gagcggcaaa acttcctgca taggtacaat
    44101 gccgatagtc aggatcgcca acgacgcact cattgttctt ccaccttact tttatttcgt
    44161 cgttaattac cgggcaagtg ttaagcacca ctgctgacac ttacttgtgc tacaaatgac
    44221 aaaaagcgta gcagcagacc tgcactgcat aatgtaaaga ttccaaatga ttccagtaat
    44281 ggattgttat tgtttaatat tctaattatt tgaaaaacat gaattgtgaa aaatgtgacg
    44341 cagatccctt gtggcggcac tcagaatccc ttccgagcgg tctgatgcgg tcactctttg
    44401 tcagggtcct catagcgttt tttcgcatcc atcgcttcct gagcggtcgg atgttcactg
    44461 atcaacgagt ctggtttagg atgatcggct ctgagttggt accaggttac cgtctgtcct
    44521 ggctttcctt tttcaatcgt cactatatat gcttcacgag gataaggcgg tttcgttggc
    44581 atcgttcttc cttatttcac gggatgaacg ttaagtatag gcgctcgaaa atcaacaatt
    44641 gatcgtctgt gccagagcgc tgcgaatttc agaaatcacc tggctgggtt cgtttgttgc
    44701 gtcgatgata atatgcgcaa cttcgcgata tagcgcatcg cgttcttcca gcacttcctg
    44761 aacttcttcg ctcagtggtt ttcccgttaa ggttggccgt aaatcttctt ccggtgcagc
    44821 ttgcagtcgg ttaaccagga ctgatactgg cgcacacaaa taaaccacga tcccgttatt
    44881 ttgcatgaag tgacgattaa attccgtcag aataatgccg ccgcccgtgg cgataacggt
    44941 ggatggcgca gttaccgctt ccagtgccgc cgtttctctg gcgcggaatc ccgcccactc
    45001 ttccctttcg acgatctccg cgaccgtcat attgagctgt gattgcaacc actggtcggt
    45061 atcgacaaac cgacggttaa gcgaatcggc aagggccatt ccgaccgttg ttttaccaca
    45121 gccccgaggc ccgatcagaa aaagaggttg tgtcatcgtg ggttttcccc aataggtcgc
    45181 aatgcggcga aagccggtgt catgagaatg gcgatcatac catcaaacta gtacaatttc
    45241 gattgtaaag aaaaaattcc acttaaagtg aaaatctcaa tacatccctt actataccaa
    45301 taaatattca agaatgaagt gtaaataata aattacattt agccacgact acgttgcact
    45361 tccagccacc acttctccag ctccgcagca aaagcctggc ggtcacgttg tgaaagacta
    45421 tctggtcctc cggtctggat cccactggca cgtaaggtat ccataaaatc gcgcatcgtc
    45481 aggcgctcac gaatggtcgc tggcgtataa cgttcgccgc gcggattaag cgccgccgcg
    45541 cctttctcaa tggcttcagc agccaaaggt atatctgcgg tgatcaccaa atcgcccgct
    45601 tcacactgcc gaacaatttc gttatcggca acgtcgaaac ctgccgcgac gcgcagcgta
    45661 cgaataaatc gtgatggcgg cacgcgtaaa ctctggtttg ctaccagtac cagcggcatc
    45721 tgcatacgtt ccgccgcgcg atacaaaatc tctttaatta cattgggaca cgcgtcggca
    45781 tccacccaaa ttgtcataaa gtcatccttt gttgggtaat cctctattgt gtcgcgcttt
    45841 tgccttccgg catagttctg tttatgcttc tgccagcgat tatcaaaaca atgaatttca
    45901 cggcaggagt gaggcaatgg aaaagaaaat cggttttatt ggctgcggca atatgggaaa
    45961 agccattctc ggcggtctga ttgccagcgg tcaggtgctt ccagggcaaa tctgggtata
    46021 taccccctcc ccggataaag tcaccgccct gcatgaacag ttcggcatca acgccgcaga
    46081 atcggcgcaa gaagtagcgc aaatcgccga catcattttt gccgccgtta aaccaggcat
    46141 catgattaaa gtgcttagcg aaatcacctc cagcctgaat aaagactctc tggtcgtttc
    46201 tattgctgca ggtatcacgc tcgaccagct tgcccgcgcg ctgggccatg accggaaaat
    46261 tatccgcgcc atgccgaaca ctcccgcgct ggttaatgcc gggatgacct ccgtaacgcc
    46321 aaacgcgctg gtaaccccag aagataccgc tgatgtgctg aatattttcc gctgctttgg
    46381 cgaagcggaa gtgattgctg agccgatgat ccacccggtg gtcggtgtga gcggttcttc
    46441 gccagcctac gtctttatgt ttatcgaagc gatggccgac gccgccgtgc tgggcgggat
    46501 gccacgcgcc caggcgtata aatttgccgc tcaggcggta atgggctccg caaaaatggt
    46561 gctggaaacg ggagaacatc cgggaacact gaaagatatg gtctgctcac cgggaggcac
    46621 caccattgag gcggtacgcg tactggaaga gaaaggcttc cgtgccgcag tgatcgaagc
    46681 gatgacgaag tgtatggaaa aatcagaaaa actcagcaaa tcctgatggc ttttgccgga
    46741 cgtcaggccg ccacttcggt gcggttacgt ccggctttct ttgctttgta gagcgccaaa
    46801 tctgccgatt tcaaccactc gcgatagtga ctcatttgtg gattcagcgg cgcaaccccc
    46861 acactaatcc gtaaagttac ctgtggcgta ttcggcaagc gtaacgtgtt tagcccttca
    46921 tgcacccgta acatggcggt gatggcgctc tcagctggcg taccggacat gattactgca
    46981 aactcatcgc cgccaaaccg accaatcaca tcgctaccgc gcagggtaat ttgtaactgt
    47041 cgggtaagcg ccacaatcgc ttcatcaccc acatcatggc cccaggtatc gttgatgctc
    47101 ttgaaatggt cgatatcgat aatcagtaac gttgcatcgc gattatgccg ccgacagtta
    47161 tcaaattcat tgcgtaacat agtttcccaa tgacgtctgt tatacacgcc cgtcataccg
    47221 tcgcgggtac tcatgacctg caacctgcgt ttatgttccg ccagtttagt cgccgtctgg
    47281 tagctgaccc agccaaacag cagaggataa atgacaataa tgggaaggga gagccaccat
    47341 tccagcggtg cgctattgaa cgacacggtg atgcccgtca gttcgagggt gacaaggcag
    47401 gaaaccacca tcaacaccag acccgcaaca aacagacggg ggccgcctgc ccccatcaaa
    47461 ttcagacaca taatcatcaa catcgcggtg gaaggcagca cgtttacgcc cattacgcct
    47521 acccacattc ccgctaatac tgcatcggtt tttaagttgt aaatttcccg gctaagcgga
    47581 tcgacggccc tgctcgctat ctgccaggct aaatgcggcc agacgaacgc ccagccgacc
    47641 aacaccagcc accaccagcc cggcggcggg tgcgaaacca gcgttgaagc aatcggtaag
    47701 aacatgccag ccagaccaac cgcgcggggt agtctgacgc gacgggcgaa gcgcagcccg
    47761 gaccgctgat gttcgttttg aggagttaaa ggaggttcct ccccatgcgc cgccgctttt
    47821 ttgaaaaagt tttcatcatt cattattttt gggaacattc agaaacaatt ttcccaaatt
    47881 atagagacgg gactaaaggc agaagtatga aatttcgggt gagccatttt cgtagctcac
    47941 ccgttagcac tatcaggtat caggcggctt tcttgaggca ggcactcata aacttattac
    48001 gatcatcccc tttcagagat tgttgtgttg cttgattatt acattcgcgc atcttttgct
    48061 gctgtggcgt caaacttttt tcaccaggcg cagatttgct gttcttcagg caatcactca
    48121 tgtaggtctt acgagcatcc cctttcaacg cctgcgccgt cgcctgctga ttacaagagg
    48181 tcatacgctg ttgttgtggg gttaaggttc tctcggcagc gccgacggtg gttaaaaaaa
    48241 ccagaccgaa aagcaaggta accagtaatg taattttcat cgcaccatcc ctcttcatgt
    48301 tttaaccatg agcgtatgcg cccgtgatct gtcattaagt ctggttgcta acagcaaaaa
    48361 aaccacccgg cagcgaaaat tcactgccgg gcgcggtttt atttcagccc cagggcggct
    48421 ttcatggtgt agaagagatc ggtctggtcg gtcagtccaa caacattggc ggcatgcggg
    48481 ccatacgccg caatacgcaa ctgactgccg gtatgttctt gtgaatcctc ttcggagttc
    48541 ccgtaactca tcaccatcac tgcgccatct ttggtattta gcgcctgggt gaggcccgga
    48601 gctttggtat ctggcgcaac tatctggctg gcatgggcgt gatcagcggt gactatgacc
    48661 aacgtgttac catccttttt agcgaattcc agcgcccgtt gtacggcttc atcgagatcg
    48721 accgtctcgc caatttgccc acaaggattc gcagcgtgat cctgtttatc gattgacgca
    48781 ccttcaactt gcaggaaaaa gcctttctca tttttactca acaattcaat ggctttgtcg
    48841 gtcatctgcg ccagggtcgg tacactgtca ttacgttgcg gattaggcgt acaggtgact
    48901 gcgggcttat ctatattacc gtggtacgtt gctttcggtc cttgccagcg cactggcata
    48961 ttgccgtcag caaacagtcc taataggggt ttttgctgat tcgcttccgt caccgaattc
    49021 agtgaggcag catcgctcac caactgataa ccacgcgcct gtgcctgttc acgcagcgtt
    49081 tttccctgcc attcaccggc ggttgccgtt tcagcaaagg tttttgcacc gccgccaagc
    49141 gtaacatcgg cacgggcgtt aagcagctgt tcggtaatcg atccttttcc gcctttttcc
    49201 agagcgttac ccggacattt ttcactggtc gcacttggac cgtagcattt gcgcgaggtc
    49261 acatgcgcca ccagcgcagc gggcgtggca tcctgcaact ctgcggtaga aacgttaccg
    49321 gtcgccagac ctgcggcttt tgccatttcc agaatcgttg ggtgatcttt ttcgtgaata
    49381 tcgacgccca gcgcgccgtt ataggttttg acaccagttg accaggcggt tgctgatgca
    49441 gccgagtcgg tgacgtagtc cggtttgccg gtttttttat tcagcgcata gtgagtgtat
    49501 tgcccggtaa gcggtaaggc atcgatacct ttaaaaaagc cgcccgcacc ttcggcataa
    49561 ttgcgtgcgg cagtaatttc cgagtccccc atcccatcgc caatcagcaa aataatattt
    49621 tttgcaggtt tatcgctaag agaatcacgc agagcagcgg tctgatcacc cgttaagcgg
    49681 cgagcaccgc cgggtgcagt aatatcgccc tgagctgccc ggttttccag aacaggcatt
    49741 tctggtgtcc gggcttttgt cacaggggta aacagtaacg gtaagagtgc cagtgcaata
    49801 gtgctttgtt tcactttatt ttctccatgt acaaatacat taaaaaataa aaacaaagcg
    49861 actataagtc tcggccgtga caactttatg acagctgttg aaaagattaa ctttttactg
    49921 acgaggatgc ctcaataact tctttacgta atcgcgcagc agctccgtat cgtcgtcagg
    49981 aatgctggca tcgggcttta cctcgtacag cgccccctct acctgatcaa tcaaccgctg
    50041 ttgatcattt tgcgccatat tgcgaagcat tgccgtgacg ataatctcca aagcttctac
    50101 ctgcgcacac agttctttcg actcttcttc tttttgggca agcttaaata acaactcagc
    50161 aatgagattt ttcatgtctg tatttcctta tccaaagtat gga
//
LOCUS       DAHHTG010000017        49434 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.017, whole genome shotgun sequence.
ACCESSION   DAHHTG010000017 DAHHTG010000000
VERSION     DAHHTG010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 49434)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49434)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49434
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.017"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            83..496
                     /locus_tag="NBI74_000998"
     CDS             83..496
                     /locus_tag="NBI74_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HCD1931366.1"
                     /translation="MKETDIAGILTSTHTIALVGASDKPDRPSYRVMKYLLDQGYHVI
                     PVSPKVAGKTLLGQQGYGTLADVPEKVDMVDVFRNSEAAWGVAQEAIAIGAKTLWMQL
                     GVINEQAAVLARDAGLNVVMDRCPAIEIPRLGLAK"
     gene            complement(541..858)
                     /gene="hspQ"
                     /locus_tag="NBI74_000999"
     CDS             complement(541..858)
                     /gene="hspQ"
                     /locus_tag="NBI74_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HspQ"
                     /protein_id="HCD1931367.1"
                     /translation="MIASKFGIGQQVRHSLLGYLGVVVDIDPVYSLSEPSPDELAVND
                     ELRAAPWYHVVMEDDNGLPVHTYLAEAQLSSELQDEHPEQPSMDELAQTIRKQLQAPR
                     LRN"
     gene            complement(916..2106)
                     /gene="rlmI"
                     /locus_tag="NBI74_001000"
     CDS             complement(916..2106)
                     /gene="rlmI"
                     /locus_tag="NBI74_001000"
                     /EC_number="2.1.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415487.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytosine(1962)-C(5))-methyltransferase
                     RlmI"
                     /protein_id="HCD1931368.1"
                     /translation="MSVRLVLAKGREKSLLRRHPWVFSGAVARMEGKASLGETIDIVD
                     HQGKWLARGAYSPASQIRARVWTFDPSESIDIAFFTRRLQQAQKWRDWLAQKDGLDSY
                     RLIAGESDGLPGITIDRFGNFLVLQLLSAGAEYQRAALISALQTLYPECAIYDRSDVA
                     VRKKEGMELTQGPITGELPPALLPIEEHGMKLLVDIQHGHKTGYYLDQRDSRLATRSY
                     VENKRVLNCFSYTGGFAVSALMGGCSQVVSVDTSQEALDIARQNVELNKLDLSKAEFV
                     RDDVFKLLRTYRDRGEKFDVIVMDPPKFVENKSQLMGACRGYKDINMLAIQLLNEGGI
                     LLTFSCSGLMTSDLFQKIIADAAIDAGRDVQFIEQFRQAADHPVIATYPEGLYLKGFA
                     CRVM"
     gene            2201..2479
                     /gene="yccX"
                     /locus_tag="NBI74_001001"
     CDS             2201..2479
                     /gene="yccX"
                     /locus_tag="NBI74_001001"
                     /EC_number="3.6.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HCD1931369.1"
                     /translation="MSKVCIIAWVYGRVQGVGFRYTTQYEAKKLGLTGYAKNLDDGSV
                     EVVACGEEGQVEKLMQWLKSGGPRSARVERVLSEPHHPSGELTDFRIR"
     gene            complement(2476..2805)
                     /gene="tusE"
                     /locus_tag="NBI74_001002"
     CDS             complement(2476..2805)
                     /gene="tusE"
                     /locus_tag="NBI74_001002"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusE"
                     /protein_id="HCD1931370.1"
                     /translation="MLIFEGKEIETDTEGYLKESSQWSEPLAVVIAENEGITLSPEHW
                     EVVRFVRDFYLEFNTSPAIRMLVKAMANKFGEEKGNSRYLYRLFPKGPAKQATKIAGL
                     PKPVKCI"
     gene            complement(2897..3556)
                     /gene="yccA"
                     /locus_tag="NBI74_001003"
     CDS             complement(2897..3556)
                     /gene="yccA"
                     /locus_tag="NBI74_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000375124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease modulator YccA"
                     /protein_id="HCD1931371.1"
                     /translation="MDRIVSSSHDRTSLLSTHKVLRNTYFLLSLTLAFSAITATASTV
                     LMLPSPGLILTLVGMYGLMFLTYKTANKPTGIISAFAFTGFLGYILGPILNTYLSAGM
                     GDVIAMALGGTALVFFCCSAYVLTTRKDMSFLGGMLMAGIVVVLIGMVANIFLQLPAL
                     HLAISAVFILISSGAILFETSNIIHGGETNYIRATVSLYVSLYNIFVSLLSILGFASR
                     D"
     gene            complement(3765..3852)
                     /locus_tag="NBI74_001004"
     tRNA            complement(3765..3852)
                     /locus_tag="NBI74_001004"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3816..3818),aa:Ser,seq:tga)
     gene            4289..5407
                     /gene="hyaA"
                     /locus_tag="NBI74_001005"
     CDS             4289..5407
                     /gene="hyaA"
                     /locus_tag="NBI74_001005"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 small subunit"
                     /protein_id="HCD1931372.1"
                     /translation="MNNEETFYQAMRRQGVTRRSFLKYCSLAATSLGLGAGMAPKIAW
                     ALENKPRIPVVWIHGLECTCCTESFIRSAHPLAKDVILSLISLDYDDTLMAAAGTQAE
                     EVFEDIITQYNGKYILAVEGNPPLGEQGMFCISSGRPFIEKLKRAAAGASAIIAWGTC
                     ASWGCVQAARPNPTQATPIDKVITDKPIIKVPGCPPIPDVMSAIITYMVTFDRLPDVD
                     RMGRPLMFYGQRIHDKCYRRAHFDAGEFVQSWDDDAARKGYCLYKMGCKGPTTYNACS
                     STRWNDGVSFPIQSGHGCLGCAENGFWDRGSFYSRVVDIPQMGTHSTADTVGLTALGV
                     VAAAVGVHAVASAVDQRRRHNQQPTETEHQPGNEDKQA"
     gene            5404..7197
                     /gene="hyaB"
                     /locus_tag="NBI74_001006"
     CDS             5404..7197
                     /gene="hyaB"
                     /locus_tag="NBI74_001006"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase large subunit"
                     /protein_id="HCD1931373.1"
                     /translation="MSTQYETQGYTINNAGRRLVVDPITRIEGHMRCEVNINDQNVIT
                     NAVSCGTMFRGLEIILQGRDPRDAWAFVERICGVCTGVHALASVYAIEDAIGIKVPDN
                     ANIIRNIMLATLWCHDHLVHFYQLAGMDWIDVLDALKADPRKTSELAQSLSSWPKSSP
                     GYFFDVQNRLKKFVEGGQLGIFRNGYWGHPQYKLPPEANLMGFAHYLEALDFQREIVK
                     IHAVFGGKNPHPNWIVGGMPCAINIDESGAVGAVNMERLNLVQSIITRTADFINNVMI
                     PDALAIGQFNKPWSEIGTGLSDKCVLSYGAFPDIANDFSEKSLLMPGGAVINGDFNNV
                     LPVDLVDPQQVQEFVDHAWYRYPNDQVGRHPFDGITDPWYNPGDVKGSDTNIQQLNEQ
                     ERYSWIKAPRWRGNAMEVGPLARTLIAYHKGDAATVESVDRMMSALNLPLSGIQSTLG
                     RILCRAHEAQWAAGKLQYFFDKLMTNLKNGNLATASTEKWEPATWPTECRGVGFTEAP
                     RGALGHWAAIRDGKIDLYQCVVPTTWNASPRDPKGQIGAYEAALMNTKMAIPEQPLEI
                     LRTLHSFDPCLACSTHVLGDDGSELISVQVR"
     gene            7216..7923
                     /gene="hyaC"
                     /locus_tag="NBI74_001007"
     CDS             7216..7923
                     /gene="hyaC"
                     /locus_tag="NBI74_001007"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase b-type cytochrome subunit"
                     /protein_id="HCD1931374.1"
                     /translation="MQQKSDNVVSHYVFEAPVRIWHWLTVLCMAVLMVTGYFIGKPLP
                     SVSGEATYLFYMGYIRLIHFSAGMIFTVVLLMRIYWAFVGNHYSRELFIVPVWRKSWW
                     QGVWYEIRWYLFLAKRPSADIGHNPIAQAAMFGYFLMSVFMIITGFALYSEHSQYAIF
                     APFRYVVEFFYWTGGNSMDIHSWHRLGMWLIGAFVIGHVYMALREDIMSDDTVISTMV
                     NGYRSHKFGKISNKERS"
     gene            7920..8507
                     /locus_tag="NBI74_001008"
     CDS             7920..8507
                     /locus_tag="NBI74_001008"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 maturation protease"
                     /protein_id="HCD1931375.1"
                     /translation="MSEQRVVVMGLGNLLWADEGFGVRVAERLYAHYHWPEDVEIVDG
                     GTQGLNLLGYVESASHLLILDAIDYGLEPGTLRTYAGERIPAYLSAKKMSLHQNSFSE
                     VLALADIRGHLPAHIALVGLQPAMLDDYGGSLSELAREQLPAAEQAALAQLAAWGIVP
                     QPANESRCLNYDCLSMENYEGVRLRQYRMTLEEQG"
     gene            8504..8902
                     /gene="hyaE"
                     /locus_tag="NBI74_001009"
     CDS             8504..8902
                     /gene="hyaE"
                     /locus_tag="NBI74_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-1 operon protein HyaE"
                     /protein_id="HCD1931376.1"
                     /translation="MSNDTPFDALWQRMLARGWTPISESRLDDWLTQAPDGVVLLSSD
                     PKRTPEVSDNPVMIGELLREFPDYTWQVAIADLEQSEAIGDRFGVFRFPATLVFTCGN
                     YRGVLNGIHPWAELINLMRGLVEPQQERAS"
     gene            8899..9756
                     /locus_tag="NBI74_001010"
     CDS             8899..9756
                     /locus_tag="NBI74_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein"
                     /protein_id="HCD1931377.1"
                     /translation="MSETFFHLLGPGTQPNDDSFSMNPLPITCQVNDEPSMAALEQCA
                     HSPQVIALLNELQHQLSERQPPLGEVLAVDLLNLNADDRHFINTLLGEGEVSVRIQQA
                     DGSESEIQEAIFCGLWRVRRRHGEQLLEDKLEAGCAPLALWQAATQNVLPTDSLLPPP
                     IDGLMNGLPLAHELLAHVRNPDAQPHSINLTQLPISEADRLFLSRLCGPGNIQIRTIG
                     YGESYINATGLRHVWHLRCTDTLKGPLLESYEICPIPEVVLAAPEDLVDSAQRLSEVC
                     QWLAEGAPT"
     gene            9890..11434
                     /gene="appC"
                     /locus_tag="NBI74_001011"
     CDS             9890..11434
                     /gene="appC"
                     /locus_tag="NBI74_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit 1"
                     /protein_id="HCD1931378.1"
                     /translation="MWDVIDLSRWQFALTALYHFLFVPLTLGLIFLLAIMETIYVVTG
                     KTIYRDMTRFWGKLFGINFALGVATGLTMEFQFGTNWSFYSNYVGDIFGAPLAMEALM
                     AFFLESTFVGLFFFGWQRLNKYQHLLVTWLVAFGSNLSALWILNANGWMQYPTGAHFD
                     IDTLRMEMTSFSELVFNPVSQVKFVHTVMAGYVTGAMFIMAISAWYLLRGRERDVALR
                     SFAIGSVFGTLAIIGTLQLGDSSAYEVAQVQPVKLAAMEGEWQTEPAPAPFHVVAWPE
                     QDQERNAFAIKIPALLGILATHSLDKPVPGLKNLMAETYPRLQRGRMAWLLMQEISQG
                     NREPHVLQAFRELEGDLGYGMLLSRYAPDMNHVTAAQYQAAMRGAIPQVAPVFWSFRI
                     MVGCGSLLLLVMLIALVQTLRGKIDQHRWVLKMALWSLPLPWIAIEAGWFMTEFGRQP
                     WAIQDILPTYSAHSALTTGQLAFSLIMIVGLYTLFLIAEVYLMQKYARLGPSAMQSEQ
                     PTQQQG"
     gene            11446..12582
                     /gene="appB"
                     /locus_tag="NBI74_001012"
     CDS             11446..12582
                     /gene="appB"
                     /locus_tag="NBI74_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HCD1931379.1"
                     /translation="MFDYETLRFIWWLLIGVILVVFMISDGFDMGIGCLLPLVARNDD
                     ERRIVINSVGAHWEGNQVWLILAGGALFAAWPRVYAAAFSGFYVAMILVLCSLFFRPL
                     AFDYRGKIADARWRKMWDAGLVIGSLVPPVVFGIAFGNLLLGVPFAFTPQLRVEYLGS
                     FWQLLTPFPLLCGLLSLGMVILQGGVWLQLKTVGVIHLRSQLATKRAALLVMLCFLLA
                     GYWLWVGIDGFVLLAQDANGPSNPLMKLVAVLPGAWMNNFVESPVLWIFPLLGFFCPL
                     LTVMAIYRGRPGWGFLMASLMQFGVIFTAGITLFPFVMPSSVSPISSLTLWDSTSSQL
                     TLSIMLVIVLIFLPIVLLYTLWSYYKMWGRMTTETLRRNENELY"
     gene            12595..12687
                     /gene="cbdX"
                     /locus_tag="NBI74_001013"
     CDS             12595..12687
                     /gene="cbdX"
                     /locus_tag="NBI74_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit CbdX"
                     /protein_id="HCD1931380.1"
                     /translation="MWYLLWFVGILLMCSLSTLVLVWLDPRLKS"
     gene            12767..14077
                     /gene="appA"
                     /locus_tag="NBI74_001014"
     CDS             12767..14077
                     /gene="appA"
                     /locus_tag="NBI74_001014"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001623747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acid phosphatase/4-phytase"
                     /protein_id="HCD1931381.1"
                     /translation="MKAILIPFLSLLIPLTPKSAFAQSEPELKLESVVIVSRHGVRAP
                     TKATQLMQDVTPDAWPTWPVKLGWLTPRGGELIAYLGHYQRQRLVADRLLAKEGCPQP
                     GQVAIIADVDERTRKTGEAFAAGLAPDCAITVHTQADTSSPDPLFNPLKTGVCQLDNA
                     NVTDAILSRAGGSIADFTGHRQTAFRELERVLNFPQSNLCLNREKQDESCSLTQALPS
                     ELKVSADNVSLTGAVSLASMLTEIFLLQQAQGMPEPGWGRITDSHQWNTLLSLHNAQF
                     YLLQRTPEVARSRATPLLDLIMTALAPHPPQKQVYGVTLPTSVLFIAGHDTNLANLGG
                     ALELNWTLPGQPDNTPPGGELVFERWRRLSDNSQWIQVSLVFQTLQQMRDKTPLSLNT
                     PPGEVKLTLAGCEERNAQGMCSLAGFTQIVNEARIPACALHQDK"
     gene            complement(14065..14277)
                     /gene="cspH"
                     /locus_tag="NBI74_001015"
     CDS             complement(14065..14277)
                     /gene="cspH"
                     /locus_tag="NBI74_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspH"
                     /protein_id="HCD1931382.1"
                     /translation="MSRKMTGIVKTFDRKSGKGFIIPSDGRKEVQVHISAFTPRDAEV
                     LIPGLRVEFCRVNGLRGPTAANVYLS"
     gene            14563..14775
                     /gene="cspG"
                     /locus_tag="NBI74_001016"
     CDS             14563..14775
                     /gene="cspG"
                     /locus_tag="NBI74_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock protein CspG"
                     /protein_id="HCD1931383.1"
                     /translation="MSNKMTGLVKWFNADKGFGFITPDDGSKDVFVHFTAIQSNEFRT
                     LNENQKVEFSIEQGQRGPAAANVVTL"
     gene            14786..14974
                     /locus_tag="NBI74_001017"
     CDS             14786..14974
                     /locus_tag="NBI74_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HCD1931384.1"
                     /translation="MTQHLHFRCPCCHGSQYRTSAFDVTERNPLGAKCIFCKSTMITF
                     DNVALQIRTDHAPLDFTK"
     gene            14949..15179
                     /gene="ymcE"
                     /locus_tag="NBI74_001018"
     CDS             14949..15179
                     /gene="ymcE"
                     /locus_tag="NBI74_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmcE"
                     /protein_id="HCD1931385.1"
                     /translation="MRRWISQNNIRLPRGAFFISALFFFNAVRIVSDNLLIIESFGEM
                     AYNISYLTRVPGTNTLLACCCLLRPEEVNSEY"
     gene            15169..15342
                     /locus_tag="NBI74_001019"
     CDS             15169..15342
                     /locus_tag="NBI74_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module toxin, GnsA/GnsB family"
                     /protein_id="HCD1931386.1"
                     /translation="MNIEELKKQAETEIADFIAQKIAELNKNTGKEVSEIRFTAREKM
                     TGLESYDVKIKIM"
     gene            complement(15391..16464)
                     /locus_tag="NBI74_001020"
     CDS             complement(15391..16464)
                     /locus_tag="NBI74_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HCD1931387.1"
                     /translation="MAEKKRTRWQRRPGTTGGKLPWNDWRNATTWRKATQLLLLAINI
                     YIAITFWYWVRYYETAGSTTFVARPGGIEGWLPIAGLMNLKYSLATGQLPSVHAAAML
                     LLVAFIVISLLLKKAFCSWLCPVGTLSELIGDLGNKLFGRQFVLPRWLDIPLRGVKYL
                     LLSFFLYIALLMPAQAIHYFMLSPYSVVMDVKMLDFFRHMGTATLISMIVLLIASLFI
                     RHAWCRYLCPYGALMGVVSLLSPFKIRRNAESCIDCGKCAKSCPSRIPVDKLIQVRTV
                     ECTGCMTCVESCPVASTLTFSLQKPAANKKAFALSGWLMTLLVMGIMFAATGYAMYAG
                     VWQSPVPEELYQRLIPQSPMIGH"
     gene            complement(16547..19279)
                     /gene="torS"
                     /locus_tag="NBI74_001021"
     CDS             complement(16547..19279)
                     /gene="torS"
                     /locus_tag="NBI74_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001054736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system sensor histidine
                     kinase/response regulator TorS"
                     /protein_id="HCD1931388.1"
                     /translation="MNLTLTRRLWMGFALMALLTLTSTLVGWYNLRFISQVEKDNTQA
                     LIPTMNMARQLSEASAWELFAAQNLTSADNEKMWQAQGRMLTAQSLKINALLQALREQ
                     GFDTTAIEQQEQEISRSLRQQGELVGQRLQLRQQQQQLSQQIVAAADEIARLAQGQAN
                     NAATSAGATQAGIYDLIEQDQRQAAESALDRLIDIDLEYVNQMNELRLSALRVQQMVM
                     NLGLEQIQKNAPTLEKQLNNAVKILQRRQIRIEDPGVRTQVATTLTTVSQYSDLLALF
                     QQDSEISNRLQTLAQNNIAQFAQFSSEVSQLVDTIELRNQHGLAHLEKASARGQYSLL
                     LLGIVSLCALILILWRVVYRSVTRPLAEQTQALQRLLDGDIDSPFPETAGVRELDTIG
                     RLMDAFRSSVHALNRHREQLAAQVKARTAELQELVIEHRQARAEAEKANQAKSAFLAA
                     MSHEIRTPLYGILGTAQLLADNPALNAQRDDLRAITDSGESLLTILNDILDYSAIEAG
                     GKNVSVSDEPFEPRPLLESTLQLMSGRVKGRPIRLATEIADDVPTALMGDPRRIRQVI
                     TNLLSNALRFTDEGHIILRSRTDGERWLVEVEDSGCGIDPAKLAEIFQPFVQVSGKRG
                     GTGLGLTISSRLAQAMSGELSATSTPEVGSCFCLRLPLRVATAPVPKTVNQAVRLDGL
                     RLLLIEDNPLTQRITVEMLNSSGAQVVAVGNAAQALETLQNSEPFAVALVDFDLPDID
                     GITLARQLAQQYPSLVLIGFSAHVIDETLRQRTSSLFRGIIPKPVPREVLAQLLAHYL
                     QLQVKNDQPLDVSQLNEDAHLMGAEKIHEWLILFKQHALPLLDEIDIARASQDNEKIK
                     RAAHQLKSSCSSLGMRSASQQCAQLEQQPLSAPLLHEEITRSVAALEAWLIRKT"
     gene            19362..20390
                     /gene="torT"
                     /locus_tag="NBI74_001022"
     CDS             19362..20390
                     /gene="torT"
                     /locus_tag="NBI74_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system periplasmic protein TorT"
                     /protein_id="HCD1931389.1"
                     /translation="MRVLLFLLLSLFMLSAFSADNLLRWHDAQHYTVQASTPLKAKRA
                     WKLCALYPSLKDSYWLSLNYGMQEAARRYGVDLKVLEAGGYSQLATQQAQIDQCKQWG
                     AEAILLGSSTTSFPDLQKQVANLPVIELVNAIDAPQVKSRVGVPWFQMGYQPGRYLVQ
                     WAHGKPLNVLLMPGPDNAGGSKEMVEGFRAAIAGSPVRIVDIALGDNDIEIQRNLLQE
                     MLERHPEIDVVAGTAIAAEAAMGEGRNLKTPLTVVSFYLSHQVYRGLKRGRVIMAASD
                     QMVWQGELAVEQAIRQLQGQSVSDNVSPPILVMTPKNADREHIRRSLSPGGFRPVYFY
                     QHTSAAKK"
     gene            complement(20363..21055)
                     /gene="torR"
                     /locus_tag="NBI74_001023"
     CDS             complement(20363..21055)
                     /gene="torR"
                     /locus_tag="NBI74_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator TorR"
                     /protein_id="HCD1931390.1"
                     /translation="MPHHIVIVEDEPVTQARLQSYFTQEGYTVSVTASGAGLREIMQN
                     QPVDLILLDINLPDENGLMLTRALRERSTVGIILVTGRSDRIDRIVGLEMGADDYVTK
                     PLELRELVVRVKNLLWRIDLARQAQPHTQDNCYRFAGYCLNVSRHTLERDGEPIKLTR
                     AEYEMLVAFVTNPGEILSRERLLRMLSARRVENPDLRTVDVLIRRLRHKLSADLLVTQ
                     HGEGYFLAADVC"
     gene            21185..22357
                     /gene="torC"
                     /locus_tag="NBI74_001024"
     CDS             21185..22357
                     /gene="torC"
                     /locus_tag="NBI74_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentaheme c-type cytochrome TorC"
                     /protein_id="HCD1931391.1"
                     /translation="MRKLWNALRRPSARWSVLALVATGIVIGIALIVLPHVGIKVTST
                     TEFCVSCHSMQPVYEEYKQSVHFQNASGVRAECHDCHIPPDIPGMVKRKLEASNDIYQ
                     TFIAHSIDTPEKFEAKRAELAEREWARMKENNSATCRSCHNYDAMDHAKQHPEAARQM
                     KVAAKDNQSCIDCHKGIAHQLPDMSSGFRKQFDELRASANDSGDTLYSIDIKPIYAAK
                     GDKEASGSLLPASEVKVLKRDGDWLQIEITGWTESAGRQRVLTQFPGKRIFVASIRGD
                     VQQQVKTLEKTTVADTNTEWSKLQATAWMKKGDMVNDIKPIWAYADSLYNGTCNQCHG
                     APEISHFDANGWIGTLNGMIGFTSLDKREERTLLKYLQMNASDTAGKAHGDKKEEK"
     gene            22357..24903
                     /gene="torA"
                     /locus_tag="NBI74_001025"
     CDS             22357..24903
                     /gene="torA"
                     /locus_tag="NBI74_001025"
                     /EC_number="1.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine-N-oxide reductase TorA"
                     /protein_id="HCD1931392.1"
                     /translation="MNNNDLFQASRRRFLAQLGGLTVAGMLGPSLLTPRRATAAQAAT
                     EAVISKEGILTGSHWGAIRATVKDGRFVAAKPFELDKYPSKMIAGLPDHVHNAARIRY
                     PMVRVDWLRKRHLSDTSQRGDNRFVRVSWDEALDMFYEELERVQKTHGPSALLTASGW
                     QSTGMFHNASGMLAKAIALHGNSVSTGGDYSTGAAQVILPRVVGSMEVYEQQTSWPLV
                     LQNSKTIVLWGSDLLKNQQANWWCPDHDVYEYYEQLKAKVAAGEIEVISIDPVVTSTH
                     EYLGREHVKHIAVNPQTDVPLQLALAHTLYSENLYDKNFLANYCVGFEQFLPYLLGEK
                     DGQPKDAAWAEKLTGIDAETIRGLARQMAANRTQIIAGWCVQRMQHGEQWAWMIVVLA
                     AMLGQIGLPGGGFGFGWHYNGAGTPGRKGVILSGFSGSTSIPPVHDNSDYKGYSSTIP
                     IARFIDAILEPGKVINWNGKSVKLPPLKMCIFAGTNPFHRHQQINRIIEGWRKLETVI
                     AIDNQWTSTCRFADIVLPATTQFERNDLDQYGNHSNRGIIAMKQVVPPQFEARNDFDI
                     FRELCRRFNREEAFTEGLDEMGWLKRIWQEGVQQGKGRGVHLPAFDDFWNNKEYVEFD
                     HPQMFVRHQAFREDPDLEPLGTPSGLIEIYSKTIADMNYDDCQGHPMWFEKIERSHGG
                     PGSQKYPLHLQSVHPDFRLHSQLCESETLRQQYTVAGKEPVFISPQDASARGIRHGDV
                     VRVFNVRGQVLAGAVVSDRYAPGVARIHEGAWYDPDKGGEPGALCKYGNPNVLTIDIG
                     TSQLAQATSAHTTLVEIEKYNGTVEQVTAFNGPVEMVAQCEYVPASQVKS"
     gene            24900..25499
                     /gene="torD"
                     /locus_tag="NBI74_001026"
     CDS             24900..25499
                     /gene="torD"
                     /locus_tag="NBI74_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone TorD"
                     /protein_id="HCD1931393.1"
                     /translation="MTTLTAQQIACVYAWLAQLFSRELDDEQLTQIASAQMAEWFSLL
                     KSEPPLTAAVNGLENSIATLTVRDDARLELAADFCGLFLMTDKQAALPYASAYKQDEQ
                     EIKRLLVEAGMETSGNFNEPADHLAIYLDLLSHLHFSLGEGTVPARRIDGLRQKTLTA
                     LREWLPEFAARCRQYDSFGFYAALSQLLLVLVECDYQKR"
     gene            complement(25754..26059)
                     /gene="cbpM"
                     /locus_tag="NBI74_001027"
     CDS             complement(25754..26059)
                     /gene="cbpM"
                     /locus_tag="NBI74_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone modulator CbpM"
                     /protein_id="HCD1931394.1"
                     /translation="MANVTVTFTITEFCLHTGISEEELNEIVGLGVVEPSEIQETTWV
                     FDDHAAIVVQRAVRLRHELALDWPGIAVALTLMDDIAHLKQENRLLRQRLSRFVAHP"
     gene            complement(26059..26979)
                     /gene="cbpA"
                     /locus_tag="NBI74_001028"
     CDS             complement(26059..26979)
                     /gene="cbpA"
                     /locus_tag="NBI74_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curved DNA-binding protein"
                     /protein_id="HCD1931395.1"
                     /translation="MELKDYYAIMGVKPTDDLKTIKTAYRRLARKYHPDVSKEPDAEA
                     RFKEVAEAWEVLSDEQRRAEYDQMWQHRNDPQFNRQFHHGDGQSFNAEDFDDIFSSIF
                     GQHARQSRQRPATRGHDIEIEVAVFLEETLTEHKRTISYNLPVYNAFGMIEQEIPKTL
                     NVKIPAGVGNGQRIRLKGQGTPGENGGPNGDLWLVIHIAPHPLFDIVGQDLEIVVPVS
                     PWEAALGTKVTVPTLKESILLTIPPGSQAGQRLRVKGKGLVSKKQTGDLYAVLKIVMP
                     PKPDENTAALWQQLADAQSSFDPRKDWGKA"
     gene            27514..28755
                     /gene="agp"
                     /locus_tag="NBI74_001029"
     CDS             27514..28755
                     /gene="agp"
                     /locus_tag="NBI74_001029"
                     /EC_number="3.1.3.10"
                     /EC_number="3.1.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glucose-1-phosphatase/inositol
                     phosphatase"
                     /protein_id="HCD1931396.1"
                     /translation="MNKTLIAATVAGIVLLASNAQAQTVPEGYQLQQVLMMSRHNLRA
                     PLANNGSVLEQSTPNKWPEWDVPGGQLTTKGGVLEVYMGHYMREWLAEQGMVKSGECP
                     PPDTVYAYANSLQRTVATAQFFITGAFPGCDIPVHHQEKMGTMDPTFNPVITDDSAAF
                     SEQAVAAMEKELSKLQLTDSYKLLEQIVNYKDSPACKEKQQCSLVDGKNTFSAKYQQE
                     PGVSGPLKVGNSLVDAFTLQYYEGFPMDQVAWGEIKSDQQWKVLSKLKNGYQDSLFTS
                     PEVARNVAKPLVSYIDKALVTERTSAPKITVLVGHDSNIASLLTALDFKPYQLHDQNE
                     RTPIGGKIVFQRWHDSKANRDLMKIEYVYQSAEQLRNADALTLQAPAQRVTLELSGCP
                     IDANGFCPMDKFDRVLNEAVK"
     gene            complement(28793..29020)
                     /locus_tag="NBI74_001030"
     CDS             complement(28793..29020)
                     /locus_tag="NBI74_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002898701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccJ family protein"
                     /protein_id="HCD1931397.1"
                     /translation="MPTQEAKAHHVGEWASLRNTSPEIAEAIFEVAGYDEKMAEKIWE
                     EGSDEVLVKAFAKTDKDSLFWGEQTIERKNV"
     gene            complement(29041..29637)
                     /gene="wrbA"
                     /locus_tag="NBI74_001031"
     CDS             complement(29041..29637)
                     /gene="wrbA"
                     /locus_tag="NBI74_001031"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HCD1931398.1"
                     /translation="MAKVLVLYYSMYGHIETMARAVAEGASKVDGAEVVVKRVPETMP
                     PQLFEKAGGKTQTAPVATPQELADYDAIIFGTPTRFGNMSGQMRTFLDQTGGLWASGA
                     LYGKLASVFSSTGTGGGQEQTITSTWTTLAHHGMVIVPIGYAAQELFDVSQVRGGTPY
                     GATTIAGGDGSRQPSQEELSIARYQGEYVAGLAVKLNG"
     gene            30010..30183
                     /locus_tag="NBI74_001032"
     CDS             30010..30183
                     /locus_tag="NBI74_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HCD1931399.1"
                     /translation="MANHRGGSGNFAEDRERASEAGKKGGQHSGGNFKNDPQRASEAG
                     KKGGKSSHGKSDN"
     gene            complement(30266..31594)
                     /gene="rutG"
                     /locus_tag="NBI74_001033"
     CDS             complement(30266..31594)
                     /gene="rutG"
                     /locus_tag="NBI74_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415526.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization transport protein G"
                     /protein_id="HCD1931400.1"
                     /translation="MAMFGFPHWQLKSTSTESGVVAPDERLPFAQTAVMGVQHAVAMF
                     GATVLMPILMGLDPNLSILMSGIGTLLFFFITGGRVPSYLGSSAAFVGVVIAATGFNG
                     QGINPNISIALGGIIACGLVYTVIGLVVMKIGTRWIERLMPPVVTGAVVMAIGLNLAP
                     IAVKNVSASAFDSWMAVMTVLCIGLVAVFTRGMIQRLLILVGLIVACLLYGVMTNVLG
                     LGKAVDFTLVSHAAWFGLPHFSTPAFNGQAMMLIAPVAVILVAENLGHLKAVAGMTGR
                     NMDPYMGRAFVGDGLATMLSGSVGGSGITTYAENIGVMAVTKVYSTLVFVAAAVIAML
                     LGFSPKFGALIHTIPAAVIGGASIVVFGLIAVAGARIWVQNSVDLSQNGNLIMVAVTL
                     VLGAGDFALTLGGFTLGGIGTATFGAILLNALLSRRLVDVPPPEVVHQKP"
     gene            complement(31615..32109)
                     /gene="rutF"
                     /locus_tag="NBI74_001034"
     CDS             complement(31615..32109)
                     /gene="rutF"
                     /locus_tag="NBI74_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309280.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization flavin reductase protein
                     F"
                     /protein_id="HCD1931401.1"
                     /translation="MNIVDQQTFRDAMSCMGAAVNIITTDGPAGRAGFTASAVCSVTD
                     TPPTLLVCLNRGASVWPVFNENRTLCVNTLSAGQEPLSNLFGGKTPMEHRFAAARWQT
                     GVTGCPQLEKALVSFDCRISQVVSVGTHDILFCAIEAIHRHATPYGLVWFDRSYHALM
                     RPAC"
     gene            complement(32120..32710)
                     /locus_tag="NBI74_001035"
     CDS             complement(32120..32710)
                     /locus_tag="NBI74_001035"
                     /EC_number="1.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonic semialdehyde reductase"
                     /protein_id="HCD1931402.1"
                     /translation="MNEAVSPGALSTLFTDARTHNGWREPPVSDETLREIYALMKWGP
                     TSANCSPARIVFIRTAEGKERLRPALSSGNLQKTLTAPVTAIVAWDSEFYERLPQLFP
                     HGDARSWFTSSPQLAEETAFRNSSMQAAYLIVACRALGLDTGPMSGFDRQYVDDAFFA
                     GSTLKSDLLINIGYGDSSKLFARLPRLSFEEACGLL"
     gene            complement(32720..33520)
                     /gene="rutD"
                     /locus_tag="NBI74_001036"
     CDS             complement(32720..33520)
                     /gene="rutD"
                     /locus_tag="NBI74_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein D"
                     /protein_id="HCD1931403.1"
                     /translation="MKLSLSPPPYADAPVVVLISGLGGSGSYWLPQLAVLDQEYQVVC
                     YDQRGTGNNPDTLAEDYSIAQMAAELHQALVAAGIERYAVVGHALGALVGMQLALDYP
                     ASVTVLVSVNGWLRINTHTRRCFQVREQLLHSGGAQAWVEAQPLFLYPADWMAARAPR
                     LEAEDALALAHFQGKNNLLRRLNALKRADFSHHADRIRCPVQIICASDDLLVPTACSS
                     ELHAALPDSQKMVMRYGGHACNVTDPETFNALLLNGLASLLHHREAAL"
     gene            complement(33528..33914)
                     /gene="rutC"
                     /locus_tag="NBI74_001037"
     CDS             complement(33528..33914)
                     /gene="rutC"
                     /locus_tag="NBI74_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein C"
                     /protein_id="HCD1931404.1"
                     /translation="MPKSVIIPAGSSAPLAPFVPGTLADGVVYVSGTLAFDQHNNVLF
                     ADDPKAQTRHVLETIRKVIETAGGTMADVTFNSIFITDWKNYAAINEIYAEFFPGDKP
                     ARFCIQCGLVKPDALVEIATIAHIAK"
     gene            complement(33926..34618)
                     /gene="rutB"
                     /locus_tag="NBI74_001038"
     CDS             complement(33926..34618)
                     /gene="rutB"
                     /locus_tag="NBI74_001038"
                     /EC_number="3.5.1.110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxyureidoacrylate/ureidoacrylate
                     amidohydrolase RutB"
                     /protein_id="HCD1931405.1"
                     /translation="MTTLTARPEAITFDPQQSALIVVDMQNAYATPGGYLDLAGFDVS
                     TTRPVIANIQTAVTAARAAGMLIIWFQNGWDEQYVEAGGPGSPNFHKSNALKTMRKQP
                     QLQGKLLAKGSWDYQLVDELVPQPGDIVLPKPRYSGFFNTPLDSILRSRGIRHLVFTG
                     IATNVCVESTLRDGFFLEYFGVVLEDATHQAGPEFAQKAALFNIETFFGWVSDVETFC
                     DALSSTSFARIA"
     gene            complement(34618..35709)
                     /gene="rutA"
                     /locus_tag="NBI74_001039"
     CDS             complement(34618..35709)
                     /gene="rutA"
                     /locus_tag="NBI74_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309285.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein A"
                     /protein_id="HCD1931406.1"
                     /translation="MKIGVFVPIGNNGWLISTHAPQYMPTFELNKAIVQKAEHYHFDF
                     ALSMIKLRGFGGKTEFWDHNLESFTLMAGLAAVTSRIQIYATAATLTLPPAIVARMAA
                     TIDSISGGRFGVNLVTGWQKPEYEQMGIWPGDDYFSRRYDYLTEYVQVLRDLWGSGKS
                     DFKGDFFTMDDCRVSPQPSVPMKVICAGQSDAGMAFSARYADFNFCFGKGVNTPTAFA
                     PTAARMKQAAEQTGRDVGSYVLFMVIADETDDAARAKWEHYKAGADEEALSWLTEQSQ
                     KDTRSGTDTNVRQMADPTSAVNINMGTLVGSYASVARMLDEVANVPGAEGVLLTFDDF
                     LSGIETFGERIQPLMQCRAHLPALTQEVA"
     gene            35997..36635
                     /gene="rutR"
                     /locus_tag="NBI74_001040"
     CDS             35997..36635
                     /gene="rutR"
                     /locus_tag="NBI74_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator RutR"
                     /protein_id="HCD1931407.1"
                     /translation="MTQGAVKTTGKRSRAVSAKKKAILSAALDTFSQFGFHGTRLEQI
                     AELAGVSKTNLLYYFPSKEALYIAVLRQILDIWLAPLKAFREDFAPLAAIKEYIRLKL
                     EVSRDYPQASRLFCMEMLAGAPLLMDELTGDLKALIDEKSALIAGWVKSGKLAPIDPQ
                     HLIFMIWASTQHYADFAPQVEAVTGATLRDEVFFNQTVENVQRIIIEGIRPR"
     gene            complement(36675..40637)
                     /gene="putA"
                     /locus_tag="NBI74_001041"
     CDS             complement(36675..40637)
                     /gene="putA"
                     /locus_tag="NBI74_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trifunctional transcriptional regulator/proline
                     dehydrogenase/L-glutamate gamma-semialdehyde
                     dehydrogenase"
                     /protein_id="HCD1931408.1"
                     /translation="MGTTTMGVKLDDATRERIKSAATRIDRTPHWLIKQAIFSYLEQL
                     ENSDTLPELPALLSGAANESDEAPTPAEEPHQPFLDFAEQILPQSVSRAAITAAYRRP
                     ETEAVSMLLEQARLPQPVAEQAHKLAYQLADKLRNQKNASGRAGMVQGLLQEFSLSSQ
                     EGVALMCLAEALLRIPDKATRDALIRDKISNGNWQSHIGRSPSLFVNAATWGLLFTGK
                     LVSTHNEASLSRSLNRIIGKSGEPLIRKGVDMAMRLMGEQFVTGETIAEALANARKLE
                     EKGFRYSYDMLGEAALTAADAQAYMVSYQQAIHAIGKASNGRGIYEGPGISIKLSALH
                     PRYSRAQYDRVMEELYPRLKSLTLLARQYDIGINIDAEEADRLEISLDLLEKLCFEPE
                     LAGWNGIGFVIQAYQKRCPLVIDYLIDLATRSRRRLMIRLVKGAYWDSEIKRAQMDGL
                     EGYPVYTRKVYTDVSYLACAKKLLAVPNLIYPQFATHNAHTLAAIYQLAGQNYYPGQY
                     EFQCLHGMGEPLYEQVTGKVADGKLNRPCRIYAPVGTHETLLAYLVRRLLENGANTSF
                     VNRIADTSLPLDELVADPVTAVEKLAQQEGQTGLPHPKIPLPRDLYGHGRDNSAGLDL
                     ANEHRLASLSSALLNSALQKWQSLPMLEQPVAAGEMSPVINPAEPKDIVGYVREATPR
                     EVEQALESAVNNAPIWFATPPAERAAILHRAAVLMESQMQQLIGILVREAGKTFSNAI
                     AEVREAVDFLHYYAGQVRDDFANETHRPLGPVVCISPWNFPLAIFTGQIAAALAAGNS
                     VLAKPAEQTPLIAAQGIAILLEAGVPPGVVQLLPGRGETVGAQLTGDDRVRGVMFTGS
                     TEVATLLQRNIASRLDAQGRPIPLIAETGGMNAMIVDSSALTEQVVIDVLASAFDSAG
                     QRCSALRVLCLQDEIADHTLKMLRGAMAECRMGNPGRLTTDIGPVIDSEAKANIERHI
                     QTMRSKGRQVFQAVRENSEDAREWQSGTFVAPTLIELDDFAELQKEVFGPVLHVVRYN
                     RNQLPELIEQINASGYGLTLGVHTRIDETIAQVTGSAHVGNLYVNRNMVGAVVGVQPF
                     GGEGLSGTGPKAGGPLYLYRLLANRPESALAVTLARQDAEYPVDAQLKAALTQPLNAL
                     REWAANRPELQALCTQYGELAQAGTQRLLPGPTGERNTWTLLPRERVLCIADDEQDAL
                     TQLAAVLAVGSQVLWPDNALHRQLVKALPSAVSERIQLAKAENITAQPFDAVIFHGDS
                     DQLRALCEAVAARDGAIVSVQGFARGESNILLERLYIERSLSVNTAAAGGNASLMTIG
                     "
     gene            40692..40901
                     /locus_tag="NBI74_001042"
     CDS             40692..40901
                     /locus_tag="NBI74_001042"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1931409.1"
                     /translation="MMLQLCATMLNVTCVASLKMNEMLINEIDMTGIKKITQTFSLRQ
                     LTFLKGATAKNVRECNLMKNSVAEH"
     gene            41060..42568
                     /gene="putP"
                     /locus_tag="NBI74_001043"
     CDS             41060..42568
                     /gene="putP"
                     /locus_tag="NBI74_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proline symporter PutP"
                     /protein_id="HCD1931410.1"
                     /translation="MAISTPMLVTFCVYIFGMILIGFIAWRSTKNFDDYILGGRSLGP
                     FVTALSAGASDMSGWLLMGLPGAVFLSGISESWIAIGLTLGAWINWKLVAGRLRVHTE
                     YNNNALTLPDYFTGRFEDKSRILRIISALVILLFFTIYCASGIVAGARLFESTFGMSY
                     ETALWAGAAATILYTFIGGFLAVSWTDTVQASLMIFALILTPVIVIISVGGFGDSLEV
                     IKQKSIENVDMLKGLNFVAIISLMGWGLGYFGQPHILARFMAADSHHSIVHARRISMT
                     WMILCLAGAVAVGFFGIAYFNGHPAVAGAVNQNAERVFIELAQILFNPWIAGILLSAI
                     LAAVMSTLSCQLLVCSSAITEDLYKAFLRKQASQKELVWVGRVMVLVVALVAIALAAN
                     PENRVLGLVSYAWAGFGAAFGPVVLFSVMWSRMTRNGALAGMIIGALTVIVWKQFGWL
                     GLYEIIPGFIFGSIGIVVFSLLGKAPSAAMQKRFAEADAHYHSAPPSRLQEG"
     gene            42788..43618
                     /locus_tag="NBI74_001044"
     CDS             42788..43618
                     /locus_tag="NBI74_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000497947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FTR1 family protein"
                     /protein_id="HCD1931411.1"
                     /translation="MFVPFLIMLREGLEAALIVSLIASYLKRTQRGRWIGVMWIGVLL
                     AAALCLGLGIFINETTGEFPQKEQELFEGIVAVIAVVILTWMVFWMRKVSRNVKVQLE
                     QAVDSALQRGNHHGWALVMMVFFAVAREGLESVFFLLAAFQQDVGVWPPLGAMLGLAT
                     AVVLGFLLYWGGIRLNLGAFFKWTSLFILFVAAGLAAGAIRAFHEAGLWNHFQEIAFD
                     MSAVLSTHSLFGTLMEGIFGYQEAPSVSEVAVWFIYLIPALVAFVLPPRAGATASRSV
                     "
     gene            43676..44803
                     /gene="efeO"
                     /locus_tag="NBI74_001045"
     CDS             43676..44803
                     /gene="efeO"
                     /locus_tag="NBI74_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake system protein EfeO"
                     /protein_id="HCD1931412.1"
                     /translation="MTINFRRNALQLSVAALFSSAFMANAADIPQVKVTVTDKQCEPM
                     TITVNAGKTQFIIQNHSQKALEWEILKGVMVVEERENIAPGFSQKMTANLQPGEYDMT
                     CGLLTNPKGKLIVKGEATADAAQSDALLSLGGAITAYKAYVMAETTQLVTDTKAFTDA
                     IKAGDIEKAKALYAPTRQHYERIEPIAELFSDLDGSIDAREDDYEQKAADPKFTGFHR
                     LEKALFGDNTTKGMDKYADQLYTDVVDLQKRISELAFPPSKVVGGAAGLIEEVAASKI
                     SGEEDRYSHTDLWDFQANVEGSQKIVDLLRPQLQKANPELLAKVDANFKKVDTILAKY
                     RTKEGFENYDKLTDADRNALKGPITALAEDLAQLRGVLGLD"
     gene            44809..46080
                     /gene="efeB"
                     /locus_tag="NBI74_001046"
     CDS             44809..46080
                     /gene="efeB"
                     /locus_tag="NBI74_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deferrochelatase/peroxidase EfeB"
                     /protein_id="HCD1931413.1"
                     /translation="MQYEDENGVNEPSRRRLLKGIGALALAGSCPVAHAQKTQSAPGT
                     LSPDARNEKQPFYGEHQAGILTPQQAAMMLVAFDVLASDKADLERLFRLLTQRFAFLT
                     QGGAAPETPNPRLPPLDSGILGGYIAPDNLTITLSVGHSLFDERFGLAPQMPKKLQKM
                     TRFPNDSLDAALCHGDVLLQICANTQDTVIHALRDIIKHTPDLLSVRWKREGFISDHA
                     ARSKGKETPINLLGFKDGTANPDSQNAKLMQKVVWVTADQQEPAWTIGGSYQAVRLIQ
                     FRVEFWDRTPLKEQQTIFGRDKQTGAPLGMQHEHDVPDYASDPEGKVIALDSHIRLAN
                     PRTAESESSLMLRRGYSYSLGVTNSGQLDMGLLFVCYQHDLEKGFLTVQKRLNGEALE
                     EYVKPIGGGYFFALPGVKDANDYLGSALLRV"
     gene            46707..47495
                     /gene="phoH"
                     /locus_tag="NBI74_001047"
     CDS             46707..47495
                     /gene="phoH"
                     /locus_tag="NBI74_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PhoH"
                     /protein_id="HCD1931414.1"
                     /translation="MGRQKAVIKARREAKRVLRRDSRSHKQREEESVTSLVQMSGVEA
                     IGMARDSRDTSPILARNEAQLHYLKAIESKQLIFATGEAGCGKTWISAAKAAEALIHK
                     DVDRIIVTRPVLQADEDLGFLPGDIAEKFAPYFRPVYDVLVRRLGASFMQYCLRPEIG
                     KVEIAPFAYMRGRTFENAVVILDEAQNVTAAQMKMFLTRLGENVTVIVNGDITQCDLP
                     RGVRSGLSDALERFEEDEMVGIVRFGKEDCVRSALCQRTLHAYS"
     gene            complement(47545..47958)
                     /gene="pgaD"
                     /locus_tag="NBI74_001048"
     CDS             complement(47545..47958)
                     /gene="pgaD"
                     /locus_tag="NBI74_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6-N-acetyl-D-glucosamine
                     biosynthesis protein PgaD"
                     /protein_id="HCD1931415.1"
                     /translation="MNNLIITTRQSPVRLLVDYVATTILWTLFALFIFLFAIDLLTGY
                     FWQSEARSRLQFYFLLAVVNAVVLIVWALYNKLRFQKQQHHAAYQYTPQEYAESLAIP
                     DELYQQLQKSHRMSVHFTSQGQIKMVVSEKTLVRV"
     gene            complement(47960..49285)
                     /gene="pgaC"
                     /locus_tag="NBI74_001049"
     CDS             complement(47960..49285)
                     /gene="pgaC"
                     /locus_tag="NBI74_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6 N-acetyl-D-glucosamine synthase"
                     /protein_id="HCD1931416.1"
                     /translation="MINRIVSFFILCLVLCIPLCVAYFHSGELMMRFVFFWPFFMSIM
                     WIIGGVYFWVYRERHWPWGENAPAPQLKDNPSISIIIPCFNEEKNVEETIHAALAQRY
                     ENIEVIAVNDGSTDKTRAILDRMAAQIPRLRVIHLAQNQGKAIALKTGAAAAKSEYLV
                     CIDGDALLDRDAAAYIVEPMLYNPRVGAVTGNPRIRTRSTLVGKIQVGEYSSIIGLIK
                     RTQRIYGNVFTVSGVIAAFRRSALAEVGYWSDDMITEDIDISWKLQLNQWTIFYEPRA
                     LCWILMPETLKGLWNQRLRWAQGGAEVFLKNMTRLWRKENFRMWPLFFEYCLTTIWAF
                     TCLVGFIIYAAQLAGVPLHIELTHIAATHTAGILLCTLCLLQFIVSLVIENRYEHNLA
                     SSLFWIIWFPVIFWMLSLATTLVSFTRVMLMPKKQRARWVSPDRGILRG"
BASE COUNT        11812 a        12692 c        13237 g        11693 t
ORIGIN      
        1 tttgctctca ttcattcgta ttagctgcat ggttggcatg tcggctttgt cgtacacttt
       61 gttaaacagt ctggaggaaa taatgaaaga aaccgatatt gcaggcattt taacgtccac
      121 tcacaccatt gcgctggtgg gggcaagcga caaacccgat cgtcccagct atcgcgtgat
      181 gaaatatcta ctcgaccagg ggtatcacgt cattccggtt tcgccaaaag ttgccggcaa
      241 aacactgctg ggccagcagg ggtatggcac gctggcagac gttccggaaa aagtcgatat
      301 ggtcgatgtt ttccgtaatt cagaagcagc atggggcgtg gcgcaggagg caatcgctat
      361 tggtgcgaaa accttatgga tgcaactggg tgttataaat gaacaggcgg cagtgctggc
      421 gcgtgatgca gggctaaacg tggtgatgga tcgctgcccg gcgattgaga tccctcgtct
      481 gggcctggca aaataaaaaa tacccggaag gcgaaaacct tccgggtatg tttatgagga
      541 ttagttacgc agacgcggcg cctggagttg tttgcggatg gtctgcgcca gctcatccat
      601 ggatggctgt tcaggatgtt catcctgcaa ttcgctactt agctgggctt cagccaggta
      661 ggtatgaacc ggtaggccgt tatcatcctc catcaccacg tgataccacg gagcggcgcg
      721 aagctcgtca ttcaccgcca gctcatcagg cgacggttca gagagcgaat aaaccgggtc
      781 gatatccacg accactccga gataacctaa cagggaatgg cggacctgct ggccgatacc
      841 gaatttgctg gcaatcatag tcacctcccg ggaaatcttt acctttgata taagggtaaa
      901 attccacatt tcaagttaca tgacgcgaca ggcaaaccct ttcagatata gcccttccgg
      961 gtaggtagcg atcaccggat gatcggctgc ctgacggaac tgctctataa attgtacatc
     1021 acgaccggca tcaattgcgg catccgcgat gattttctga aataaatcgc tggtcatcag
     1081 accggaacag gagaaagtca ggagaatacc gccttcattt agcaactgaa tcgccagcat
     1141 gttgatgtct ttataaccac gacacgcgcc catcaactgg cttttattct caacaaactt
     1201 cggcgggtcc atcacgataa catcaaattt ttcaccgcga tcgcgataag tacgcagcaa
     1261 tttaaagaca tcatcacgga caaactctgc cttgctcaga tccagtttgt tcagctcaac
     1321 gttctgccgt gcaatatcca gcgcttcctg ggaggtatca acgctgacaa cctggctgca
     1381 accgcccatc aatgccgata cggcgaaacc accggtatag gagaaacagt tcagtacgcg
     1441 tttattttca acgtagctgc gggtagccag gcggctgtct cgctggtcca ggtagtaacc
     1501 cgttttgtgc ccgtgctgaa tatccaccag cagtttcatg ccgtgttctt caatcggcag
     1561 cagggcaggc ggcaactcgc cggtgatggg gccctgagtc agctccatcc cttctttttt
     1621 acgtaccgcg acgtcgctgc gatcgtaaat ggcacattcc gggtacaacg tttgcagggc
     1681 gctaattaat gccgcgcgct gatactctgc gccagcactc agcaattgca gcaccagaaa
     1741 attaccgaaa cgatcgatag taatacccgg caggccatca gattcaccgg cgattaaacg
     1801 atagctgtcg aggccatctt tttgcgccag ccagtcacgc catttttgtg cttgctgcaa
     1861 acggcgggta aaaaaagcaa tgtcgataga ctcagacggg tcaaacgtcc agacgcgcgc
     1921 ccggatttgc gaagctggcg aataagcgcc gcgtgctaac cattttccct gatgatcaac
     1981 aatatcgatg gtttcaccga ggctggcttt accttccatg cgggcaacgg ccccggaaaa
     2041 gacccacgga tggcgacgaa gtaatgattt ttcgcgccct ttggctaaca ctaaacgtac
     2101 actcataatt tacttttctg tcgatgcaaa gaaatgggcg tcattgtccg gagttttcca
     2161 tggatttgca acgcgtccat taaggataag gagagcagca atgtcgaaag tctgcataat
     2221 tgcctgggtt tacgggcgag ttcagggcgt aggattccgc tacaccacgc agtacgaagc
     2281 gaaaaaactg ggactaaccg ggtacgccaa aaatcttgac gatggcagcg tggaagtggt
     2341 tgcctgcggt gaagaagggc aggtggaaaa attaatgcag tggttaaaaa gtggcggccc
     2401 gcgttctgcg cgtgtggaac gggtgctcag cgagccgcat catccctccg gggagttaac
     2461 ggattttcgt atccgttaaa tgcattttac cggtttaggc aggccagcaa ttttggtggc
     2521 ttgctttgcc ggacctttcg ggaacagtcg gtacagatag cggctattgc ctttctcttc
     2581 gccaaattta ttcgccatcg cttttaccag catacgaatc gccggagaag tattgaattc
     2641 cagatagaaa tcacgcacaa aacgcaccac ttcccagtgt tctggcgaca gagtaatccc
     2701 ttcgttttct gcaatcacca ccgccagcgg ctcactccac tggctgcttt ctttgagata
     2761 gccttcggta tccgtttcta tctctttacc ttcgaagatc agcataattc tttactacct
     2821 taatcagact ggcggcagtg taacaaaaaa caaagccccg cataaagcga ggctcggtaa
     2881 gtaaccctgg gcgagattaa tcgcggctgg cgaagcccag aatgctcagc aggctgacga
     2941 agatgttgta cagcgaaaca tacaggctaa ccgtggcacg aatatagttc gtttcaccgc
     3001 catgaatgat gttgctggtt tcaaacaaga tagcgccaga ggagatcaga atgaagaccg
     3061 cgctgatcgc cagatgcaga gcaggcagtt gcaggaagat attagcaacc ataccaatca
     3121 gcaccaccac aatacccgcc atcagcatac cgccgaggaa agacatatct ttgcgggtgg
     3181 tcagcacata cgcagagcag cagaagaaca ctaacgccgt tccgcccagt gccatagcga
     3241 ttacgtcacc cattccggca gacagatagg tgttcagaat tggtccgagg atataaccca
     3301 gaaaaccggt aaaggcgaat gcggagataa tcccggtcgg cttattcgcc gttttgtagg
     3361 tcaggaacat caaaccatac atacccacca gcgtcagaat cagacccgga gatggcagca
     3421 tcagcaccgt actggcagtt gcggtaatcg ccgaaaaggc cagcgtcagg ctcagcagaa
     3481 aataggtatt acgcagcacc ttatgggtgc taagcagcga tgtacggtca tgtgaagaac
     3541 taactatacg atccattgag tcactctcta tgacagatgt aattaattaa gcagcataat
     3601 gataatgcgt aagggcaccc agaagtttta cccatcttta cgcatttgat ctggaacagg
     3661 tttaacagcg gattatcagg tcattaggca gtgatataac gccctgagaa tttcgacagg
     3721 caaaagaaaa aggggttagc atttagctaa ccccttatct tatttggcgg aagcgcagag
     3781 attcgaactc tggaaccctt tcgggtcgcc ggttttcaag accggtgcct tcaaccgctc
     3841 ggccacactt ccggaatgac gcgcactata aacatcccga tgcggcgtgt aaacccctaa
     3901 tttgtttgtt tgcctgaaaa atagccaaaa gtgcattgat agcgtgaaac aacagcagat
     3961 tgatcatttc atcaccatga attccttctc tatttactcg tttagcaacc ggctaaacat
     4021 ccccaccacc cggcaaaaag aaaaaaaaga aaaataggtc catttttatc gctaaaagat
     4081 aaatccacac agtttgtatt gttttgtgca aaagtttcac tacgctttat taacaatact
     4141 ttctggcgac gtgcgccagt gcagaaggat gagctttcgt tttcagcatc tcacgtgaag
     4201 cgatggtttg ccttgctaca gggacgtcgc ttgccgacca tgtgcgcccg gtgttctgcc
     4261 ggtgtcgcaa ggagaagaga cgtgcgatat gaataacgag gaaacatttt accaggccat
     4321 gcggcgtcag ggcgttaccc ggcgcagctt tctcaaatat tgtagtctgg ctgccacgtc
     4381 gctgggatta ggcgcgggaa tggcaccaaa gattgcctgg gcgctggaga ataaaccgcg
     4441 cattccggtg gtatggatcc acggcctgga atgcacctgc tgtaccgaat cttttatccg
     4501 ctccgctcac ccactggcga aggacgtcat cctttccctg atttccctcg attacgacga
     4561 taccctgatg gctgccgccg gaacccaggc ggaagaagtc tttgaagaca tcatcacgca
     4621 atacaatggc aaatatatcc tcgcagtaga aggtaatccg ccgctgggcg agcaggggat
     4681 gttctgtatc agcagcggac gaccgtttat tgagaaactc aagcgtgccg ccgctggagc
     4741 cagtgcgatt atcgcctggg gaacctgcgc gtcctggggc tgcgtgcagg ccgcgcgacc
     4801 taatccgacg caggcaacgc ctatcgacaa agtcatcacc gacaaaccca ttatcaaagt
     4861 acctggctgc ccgccgatcc cggatgtgat gagcgccatc attacttaca tggtgacgtt
     4921 tgatcgtctg ccggatgtcg acagaatggg ccgtccactg atgttttatg gtcagcgtat
     4981 ccacgataaa tgctatcgcc gtgctcactt cgacgccgga gagttcgtcc agagttggga
     5041 tgatgacgct gcccgcaaag gttactgcct gtacaaaatg ggctgcaaag ggcctaccac
     5101 ctataacgcc tgttcctcca cacgctggaa cgatggcgtt tccttcccga tccagtcagg
     5161 tcacggctgc ctgggttgtg cggaaaatgg tttctgggat cgcggttcgt tctacagccg
     5221 cgtggtcgat attccacaaa tgggtaccca ttccaccgcc gacaccgtcg gtttaaccgc
     5281 gcttggcgtg gtggcagcgg ctgttggtgt gcacgcagtc gccagcgccg ttgaccagcg
     5341 cagacgtcat aaccagcaac ctacagaaac cgaacatcag ccaggcaatg aggataaaca
     5401 ggcatgagca ctcagtacga aactcaggga tacaccatca acaatgccgg acgccgcctg
     5461 gtggtcgacc cgattacgcg cattgaaggc cacatgcgct gcgaagtgaa tattaacgat
     5521 cagaatgtga tcaccaatgc cgtctcctgc ggcaccatgt ttcgcgggct ggagatcatt
     5581 ctgcaagggc gtgacccgcg cgatgcgtgg gcgttcgttg aacgtatctg cggcgtctgt
     5641 actggcgtac acgccctggc ttcggtttac gccatcgaag atgccatcgg tattaaagtg
     5701 ccggacaacg ccaatatcat ccgcaacatt atgctggcaa cgctctggtg ccacgatcat
     5761 ctggtgcact tctatcagct tgccgggatg gactggatcg atgtgttaga tgcgctgaaa
     5821 gccgacccgc ggaaaacctc cgaactggca caaagcctct cctcatggcc gaaatcatcc
     5881 cctggctatt tcttcgacgt acaaaaccgc ctgaagaaat tcgttgaagg cgggcagttg
     5941 gggatcttcc gcaatggcta ctgggggcac ccgcagtaca aactgccgcc agaagccaac
     6001 ctgatgggct ttgcccacta tctcgaagct ctcgatttcc agcgtgaaat tgtcaaaatc
     6061 cacgcggtct ttggtggtaa aaacccgcat ccaaactgga ttgtcggcgg gatgccttgc
     6121 gccattaaca ttgacgaaag cggcgcagtc ggggcggtca atatggaacg cctgaacctg
     6181 gtgcagtcga tcattacccg tacggcggac ttcattaaca acgtgatgat ccccgacgcc
     6241 ttagccatcg gccagttcaa caaaccgtgg agcgaaatcg gcacgggtct ttctgataaa
     6301 tgtgtcctca gctacggcgc attcccggat attgccaacg actttagtga gaaaagtctg
     6361 ctgatgcctg gcggcgcggt gattaacggc gacttcaaca atgtgctgcc agtggatttg
     6421 gttgatccgc agcaggtgca ggagtttgtc gaccacgcct ggtatcgtta tcccaacgat
     6481 caggtggggc gtcatccgtt cgatggcatt accgatccgt ggtacaaccc cggcgatgtc
     6541 aaaggcagcg ataccaacat tcagcagctg aatgaacagg aacgctactc gtggatcaaa
     6601 gcgccacgct ggcgcggtaa cgcgatggaa gtggggccgc tggcacgcac gttaatcgct
     6661 tatcacaaag gcgatgctgc gaccgttgag tcggtcgatc gcatgatgtc ggcgttgaac
     6721 ctgccgcttt ccggcatcca gtcaacgttg ggccgcattt tgtgtcgcgc gcacgaagcg
     6781 cagtgggccg caggtaagtt gcagtatttc ttcgataaac tgatgaccaa cctgaaaaac
     6841 ggcaatcttg ccaccgcttc cacggaaaaa tgggaaccag caacctggcc gacagagtgc
     6901 cgtggtgtcg gttttaccga agcgccgcgc ggggcgttag gccactgggc tgccattcgc
     6961 gatggcaaga ttgatctcta ccagtgcgtg gtaccgacca cctggaacgc cagcccgcgc
     7021 gatcccaaag ggcagattgg cgcttatgaa gcggcgctga tgaacaccaa aatggcgatc
     7081 cccgagcaac cgctggagat cctgcgtact ctgcacagct ttgatccgtg cctcgcctgt
     7141 tcaacacacg tgctgggcga cgacggtagc gagctgatct ccgtgcaggt gcgttaacag
     7201 cgaaggagaa tcatcatgca acagaaaagc gacaacgttg tcagtcacta tgtctttgaa
     7261 gcacccgtgc ggatatggca ctggctgaca gtgttatgca tggcggtgtt gatggtcacc
     7321 ggttacttta tcggcaagcc gctaccttcc gtcagcggcg aggcgacgta tctgttctat
     7381 atgggctaca tcaggttaat tcacttcagt gccgggatga tttttaccgt ggttttgctg
     7441 atgcggatct actgggcttt tgttggcaat cattactccc gcgagctgtt tatcgtgccg
     7501 gtatggcgta aaagctggtg gcagggcgtg tggtatgaaa tccgctggta tctgtttctg
     7561 gcaaaacgtc cgagtgccga tataggccat aaccccatcg cccaggcggc gatgttcggc
     7621 tatttcctga tgtcagtttt tatgatcatc accggctttg cgctgtacag cgaacacagc
     7681 cagtacgcta tttttgcgcc gttccgttat gtggtggaat ttttctactg gacgggcggc
     7741 aactcaatgg acattcacag ctggcatcgg ctggggatgt ggctgattgg cgcatttgtg
     7801 atcggccatg tctacatggc gctgcgtgaa gacatcatgt ccgacgacac ggtgatctcc
     7861 actatggtca acggctaccg tagccacaaa tttggcaaaa taagtaacaa ggagcgttca
     7921 tgagcgagca acgcgtggtg gtaatggggc tgggcaacct gctgtgggcc gatgaaggct
     7981 tcggcgtgcg ggtggcggaa cggctgtatg cccattacca ctggcccgag gatgtggaga
     8041 ttgtcgatgg cggcacccaa ggactgaact tgctggggta tgtcgaaagc gccagtcatc
     8101 tgttgattct cgatgccatt gattacgggc tggaacccgg aacgctacga acgtatgccg
     8161 gagaacgcat tccggcttat ctcagcgcga agaaaatgag cctgcaccag aacagtttct
     8221 ccgaagtgct ggcgctggca gatatccgcg gacatctgcc agcacatatt gccctcgtcg
     8281 gtctgcaacc cgcgatgctc gacgactacg gcggcagcct gagcgaactg gcgcgggagc
     8341 aactgcccgc cgcggaacag gcggcgctgg cgcagctggc tgcgtgggga attgtgccgc
     8401 aaccggctaa tgaatcgcgc tgtctcaatt atgactgtct gtcgatggaa aattacgaag
     8461 gcgttcgttt gcgccagtac cggatgacac tggaggagca gggatgagca acgacacgcc
     8521 atttgatgcg ttgtggcaac gaatgctggc gcgcggctgg acgccgatca gtgaatcccg
     8581 tcttgacgac tggcttacgc aagcgccaga cggcgtggtg ttattaagca gcgatccgaa
     8641 acgcacgcca gaagtcagcg ataacccggt aatgattggc gaattattgc gcgagtttcc
     8701 cgattataca tggcaggtag cgattgctga ccttgagcag agcgaagcca tcggcgatcg
     8761 ctttggcgtc tttcgctttc ccgccacatt agtatttacc tgcggaaact atcgcggcgt
     8821 gctgaatggt attcacccgt gggcggaact gataaacctg atgcgcggac ttgtcgaacc
     8881 gcagcaggag cgtgcctcat gagcgaaact tttttccatc tgctggggcc aggaacgcaa
     8941 ccgaacgatg acagtttcag catgaatcca ctgccgatca cctgtcaggt gaatgatgaa
     9001 ccgagtatgg cggccctgga gcaatgtgct cacagcccgc aggtgattgc gctgttaaac
     9061 gagttacaac atcaactaag cgaacgccag ccgccgttgg gcgaggtact ggcagtcgat
     9121 ctgttaaatc tcaacgccga cgatcgtcac tttatcaata cgcttctcgg ggaaggggaa
     9181 gtgtcagtgc gcattcagca ggctgatggt agtgaaagtg aaattcagga ggcgatcttc
     9241 tgcggattat ggcgggtacg cagacgtcac ggcgaacagt tgctggaaga caaactggag
     9301 gctggctgcg caccgctggc gttgtggcag gcggcaacgc aaaacgtctt gccgacagat
     9361 tcgctgttac cgccgcccat tgatggcctg atgaatggcc taccgttggc ccatgaatta
     9421 ctggcacatg tacgtaaccc cgacgcacag ccgcacagca ttaatctgac gcaattaccc
     9481 atcagcgaag ctgaccggct ttttctctca cgtctctgtg ggccgggaaa tattcagatt
     9541 cgtaccattg gctatggcga gagctatatc aacgccacgg ggttacgcca tgtctggcat
     9601 ttacgctgta cggacacctt aaaaggcccg ttactggaaa gttatgaaat ctgcccaata
     9661 ccggaagtgg tgctggcagc gccagaagat ttggtcgact ctgcgcagcg gcttagcgag
     9721 gtatgccagt ggctggcgga aggtgcaccg acgtaaaaag acggtaagta tcgctttcag
     9781 gcttatgaat atcgcaatcg gcgaatacct ctggtcgtag agtttcagga taaagaggga
     9841 gatctaccat taacgggtta tttttctctc ttcgcctaca ggagtgcgca tgtgggatgt
     9901 cattgattta tcgcgctggc agtttgctct gaccgcgctg tatcactttt tatttgtacc
     9961 ccttaccctg gggctgattt ttttgctggc tatcatggaa accatttacg tggtcaccgg
    10021 caaaacaatc taccgcgata tgacgcgctt ctggggtaag ctcttcggta tcaactttgc
    10081 tcttggcgtg gctaccggcc tgacgatgga gtttcagttt ggtactaact ggtcattcta
    10141 ttccaactat gtgggcgata tttttggcgc accactggcg atggaagcat taatggcctt
    10201 cttcctcgaa tccacctttg tcgggctgtt cttcttcggc tggcaacggc tgaataaata
    10261 ccagcacctg ctggtgacgt ggctggtggc gttcggttca aatctctctg cgttgtggat
    10321 attgaatgcc aacggttgga tgcaataccc gactggtgcg cattttgata tcgacaccct
    10381 gcgtatggag atgaccagtt tcagcgagct ggtctttaac ccggtcagcc aggtgaaatt
    10441 tgtgcacacc gtaatggcgg gatacgtgac cggggccatg tttattatgg cgatcagcgc
    10501 ctggtattta ctgcgcggac gggagcgcga tgtcgcatta cgctcgtttg ccatcggttc
    10561 cgtcttcggt actctggcga ttatcggtac gctgcaactc ggagacagtt ctgcgtatga
    10621 agtcgcgcaa gtacaaccgg taaaactggc ggcgatggaa ggggagtggc aaacagaacc
    10681 tgcacctgca ccgttccatg tggttgcctg gccggaacag gatcaagagc gtaacgcctt
    10741 tgccatcaaa attcccgcgc tgctagggat cctcgccact cactcattag ataaacccgt
    10801 gccgggtctg aagaatttga tggctgaaac ctaccctcgc ttgcaacgtg gacgtatggc
    10861 ctggctgtta atgcaggaaa tatcgcaagg caatcgtgag ccgcatgtgt tgcaggcatt
    10921 ccgggagctg gaaggcgacc tgggttacgg catgttgctc tcccgctatg cgccggatat
    10981 gaatcatgtc acagccgcac agtaccaggc ggcgatgcgt ggcgcgatac ctcaggttgc
    11041 gccggtattc tggagtttcc gcatcatggt gggctgtggt tccctgctgc tactggtgat
    11101 gctgattgcg cttgtccaga cgctgcgtgg caaaatcgac cagcatcgct gggtgctgaa
    11161 aatggcgctc tggagtttgc cgctgccgtg gattgcgatt gaagccggat ggtttatgac
    11221 cgagtttggt cgtcagccgt gggcgataca ggacatctta ccgacatact ccgcgcactc
    11281 cgccttaacc acaggacaac tggctttctc actgatcatg atcgtagggc tttacaccct
    11341 gttcttaatc gccgaagtct acctgatgca gaaatatgcc cgtctggggc cgagcgcgat
    11401 gcagagtgaa caaccgacgc agcaacaggg gtaaaggaga aaatcatgtt tgattatgaa
    11461 acattgcgct tcatctggtg gctgctgatt ggcgtgatcc tggtggtctt tatgatctcc
    11521 gacggatttg acatggggat cggctgtctg ctgccgctgg tggcgcgtaa tgatgatgaa
    11581 cgtcggatag tgataaacag cgttggcgcg cactgggaag gcaatcaggt ctggttgatc
    11641 ctcgcaggtg gggcattatt tgccgcctgg cccagagtgt atgcagcggc gttttccggc
    11701 ttttatgtgg cgatgattct ggtgctgtgc tcgctgttct ttcgcccgct ggcctttgat
    11761 tatcgcggaa aaatcgccga tgcacgctgg cgtaaaatgt gggatgccgg actggtcatc
    11821 ggcagtctgg tgccgccggt agtcttcggt attgcattcg gcaacttgct gctcggtgtg
    11881 ccgtttgcct tcacaccgca attacgcgtg gaatatctcg gcagcttctg gcaactgctg
    11941 acgccattcc ctttattgtg tggattgctc agtcttggga tggtgatttt gcaaggtggc
    12001 gtctggttac aactgaaaac tgttggtgtg attcatctgc gttcacagct ggcgaccaaa
    12061 cgcgccgcac tactggtgat gctgtgcttt ttgctggcgg gttactggct gtgggttggt
    12121 attgatggct ttgtgctgct cgcccaggat gctaacggtc cttccaatcc gttaatgaaa
    12181 ctggtggcag tgctacctgg tgcctggatg aataatttcg tcgaatcgcc cgttttgtgg
    12241 atcttcccgc tgctggggtt cttctgccca ttgctgacgg tgatggcgat ttatcgtggt
    12301 cgcccgggtt ggggattttt gatggcgtca ttgatgcaat ttggcgtgat tttcacggca
    12361 ggcatcacgc tgttcccctt tgtcatgccg tcaagcgtga gtccgatctc cagcctgacg
    12421 ttgtgggaca gcacttccag tcagctgacg ctgagcatta tgttggtaat cgtgctgata
    12481 tttttgccca ttgtgttgct ctacactctc tggagctact acaaaatgtg ggggcgcatg
    12541 acgacagaaa ctctccgccg taacgaaaac gagctgtact aaggagcagg aacaatgtgg
    12601 tatttacttt ggttcgtcgg cattttgttg atgtgttcac tctccaccct tgtgttggta
    12661 tggctggacc cgcgtctgaa aagttaacga aggtaagcct gatgaggcgc attagcgtcg
    12721 catcaggcaa tcaataatgt cggatatgaa aagcggaaac atatcgatga aagcgatctt
    12781 gatcccattc ttatctcttc tgattccgtt aacaccgaaa tctgcattcg ctcagagtga
    12841 gccggagctg aagctggaaa gtgtggtgat tgtcagtcgt cacggtgtgc gtgcaccaac
    12901 caaggccacg caactgatgc aggatgtcac ccctgacgca tggccaacct ggccggtaaa
    12961 actgggttgg ctgacaccgc gcggtggtga gctaatcgct tatctcggac attaccaacg
    13021 ccagcgtctg gtggccgaca gattgctggc aaaagagggc tgcccgcagc caggtcaggt
    13081 cgcgattatt gctgatgtcg acgaacgtac ccgtaaaaca ggcgaagcct tcgccgccgg
    13141 gctggcacct gactgtgcaa taaccgtaca tacccaggca gatacgtcca gtcccgatcc
    13201 gttatttaat cctctaaaaa ctggcgtttg ccaactggat aacgcgaacg tgactgacgc
    13261 gatcctcagt agggcaggag ggtcaattgc tgactttacc gggcatcggc aaacggcgtt
    13321 tcgcgaactg gaacgggtgc ttaatttccc gcaatcaaac ttgtgcctta accgtgagaa
    13381 acaggacgaa agctgttcat taacgcaggc attaccatcg gaactcaagg tgagcgccga
    13441 caatgtctca ttaaccggtg cggtaagcct cgcatcaatg ctgacggaga tatttctcct
    13501 gcaacaagca cagggaatgc cggagccggg gtggggaagg atcaccgatt cacaccagtg
    13561 gaacaccttg ctaagtctac ataacgcgca gttttatttg ctgcaacgca caccagaggt
    13621 tgcccgcagt cgcgccaccc cgttattgga tttgatcatg acagcgttgg cgccccatcc
    13681 accgcaaaaa caggtgtatg gtgtgacatt acccacttca gtgctgttta tcgccggaca
    13741 cgatactaat ctggcaaatc tcggcggcgc actggagctc aactggacgc ttccaggtca
    13801 gccggataac acgccgccag ggggtgaact ggtgtttgaa cgctggcgtc ggctaagcga
    13861 taacagccag tggattcagg tttcgctggt cttccagact ttacagcaga tgcgtgataa
    13921 aacgccgctg tcattaaata cgccgcccgg agaggtgaaa ctgaccctgg caggatgtga
    13981 agagcgaaat gcgcagggca tgtgttcgtt ggccggtttt acgcaaatcg tgaatgaagc
    14041 acgcatacca gcgtgcgctc tccatcaaga caaataaaca tttgccgctg ttggtccacg
    14101 caggccattt acgcggcaaa attccacgcg taatcctggt ataagcactt ctgcgtcgcg
    14161 gggagtgaat gcggaaatat ggacctggac ttctttacga ccgtcggagg ggataatgaa
    14221 tcctttgccg cttttgcgat caaaggtttt gacaattcct gtcattttac gggacaaaca
    14281 aattccttac tgaaaatact gcgctgcact atacggggtt aataaaataa agccagcgat
    14341 atttaagacc gccggacggc taaaataaaa tttgcttaat ctcaattatc atgcgttaat
    14401 agctgcgtcg gtttgaaaga cagacagcat acaaagtagt ttactaaagc agttctcatt
    14461 atcaggcatt atccccttct tttgagtctc tctcctgaac actaagtagt ttctgtatta
    14521 aagccctgtt tgccgaaagg cccaaaatga aggaagtaaa atatgtctaa taaaatgact
    14581 ggtttagtaa aatggtttaa cgcagataaa ggttttggct ttatcactcc tgatgatggc
    14641 agcaaagacg ttttcgtcca tttcactgcc atccagagca atgaattccg cacgctgaac
    14701 gaaaatcaga aagttgaatt ttctattgag caggggcaac gtggtcccgc ggcagcgaac
    14761 gttgttacgc tctaaggttg ccgttattac tcaacatctc catttccgct gtccatgttg
    14821 ccatggttca cagtaccgca catcggcatt cgatgtgacg gagcgaaacc ctttgggtgc
    14881 aaagtgcatt ttttgtaaat cgacgatgat cacctttgat aacgttgcgc tgcaaatacg
    14941 cactgaccat gcgccgctgg atttcacaaa ataatatcag gctccctcgt ggagcctttt
    15001 ttatatctgc cttatttttc ttcaatgctg tacgtatagt aagcgataac ctgttgatta
    15061 ttgaatcttt cggggagatg gcttataaca tttcttacct gaccagggta ccgggaacca
    15121 acaccttact ggcgtgttgc tgtcttttaa gaccagaaga ggttaacagt gaatattgaa
    15181 gagttaaaaa aacaagccga aacggaaatc gccgacttta tcgcgcaaaa aatcgccgag
    15241 ctgaacaaga atacagggaa agaagtctct gaaattcgct tcaccgcacg agaaaaaatg
    15301 accgggcttg aaagttatga tgtcaaaatc aaaataatgt gattttgtga acatcacccc
    15361 gtgcgaggtg atgttccgct tgttgctaat ttagtgacca atcattggcg attgcggaat
    15421 taagcgttgg tacaattcct ccggcaccgg gctttgccat actcccgcat acattgcgta
    15481 accagtcgcc gcaaacataa tccccataac cagtagcgtc attaaccagc cagacaacgc
    15541 aaaggctttt ttatttgccg caggtttttg cagtgaaaag gtcaatgttg aggctaccgg
    15601 acatgactct acgcaagtca tacagccggt acattccact gttcgtacct gaattaattt
    15661 atcgaccggg atccgtgatg ggcaactttt tgcgcatttg ccacaatcga tacaactttc
    15721 ggcattgcga cgaatcttaa acggcgataa tagcgaaacc acgcccatca gcgcgccata
    15781 tgggcaaaga taacgacacc aggcatggcg aataaacagg ctggcaatca gcaaaacgat
    15841 catgctgatt aatgtcgcgg tccccatatg acgaaagaaa tcgagcattt taacgtccat
    15901 caccacgctg tagggcgaca acataaaata gtgaatcgcc tgagcgggca tcaataacgc
    15961 gatatagaga aaaaaactca acagcaaata cttcacgccg cgcagaggaa tatccagcca
    16021 gcggggaagg acaaattgcc gaccaaacag tttgttaccg agatcgccga ttaattccga
    16081 aagcgtacca accgggcata accatgaaca aaaggccttt ttgagtaata gactgatgac
    16141 gataaaagcg accaataaca gcatcgcggc ggcgtggacg gacggtaact gacctgttgc
    16201 aaggctatat ttcagattca tcagaccggc aatcggtagc cagccttcga tacctcccgg
    16261 tctggcgaca aatgtcgtac tacctgccgt ttcgtaatag cgcacccaat accagaacgt
    16321 gatggcaata taaatattaa tagccaacag taataattgc gtcgctttac gccaggtcgt
    16381 ggcattgcgc cagtcattcc acggtaattt gccgcccgtc gtgcctggcc gccgctgcca
    16441 gcgggttctt tttttctctg ccatgatttt gccagtccgt taagttgcat accaaatgcc
    16501 actattctag ttgttcttaa ctggctgata ttgattcaaa tcgcgttcag gtctttctta
    16561 ttaaccatgc ttccagagcg gcaacactgc gtgtaatttc ttcgtgtaga aggggggcag
    16621 ataatggctg ctgctccagt tgtgcgcatt gctggctggc gctacgcatt cccagactcg
    16681 agcaactgct ttttagctgg tgtgcggcac gctttatttt ctcgttatcc tgactggcgc
    16741 gggcaatgtc gatttcatcg agaagcggca gggcatgttg tttaaataat atcagccatt
    16801 cgtggatctt ctccgccccc attaaatgag catcttcatt gagttgcgat acatccagcg
    16861 gttgatcatt tttgacttgc agttggagat agtgcgccag taattgagcg agcacttcac
    16921 gcggcaccgg tttagggata atcccgcgaa atagcgaact ggtacgctgg cgcagtgttt
    16981 cgtcgatgac atgagcgcta aagccaatca aaaccagcga cggatattgc tgtgccagtt
    17041 gccgggcaag cgtaatgccg tcgatatccg gcagatcaaa atccaccagt gcgacagcaa
    17101 atggttcact attttgcagt gtctctaaag cctgcgcggc atttcctacg gcaacgacct
    17161 gcgccccact gctgttcaac atctcaacgg taattcgctg ggttagcggg ttatcttcaa
    17221 ttaacagcaa acgtaaaccg tccagacgca ccgcctgatt gactgttttt ggcacgggtg
    17281 cagtggcaac acgtaacggc aagcgtaaac aaaaacagct tccaacctcc ggcgtgctgg
    17341 tggcgctcag ttcgccgctc atcgcctggg ccagacggct actgatagtc agccccagcc
    17401 cggtgccgcc gcgtttgccg ctaacctgaa caaatggctg gaagatttca gccagtttcg
    17461 cgggatcaat accgcagccg ctgtcttcca cttcgaccag ccaccgctcg ccatcagtgc
    17521 gactacgcag gataatgtgc ccttcgtcgg tgaaacgcag ggcgttgctc aacaggttgg
    17581 taatgacctg acgaatacgc cgcggatcgc ccattaacgc agtcggtaca tcgtcggcaa
    17641 tttcagttgc caggcgaatc gggcgacctt tcacccgtcc gctcattaat tgcagggtac
    17701 tttccagcaa cgggcgcggt tcaaagggtt catcgctgac cgaaacattc ttgccgcctg
    17761 cttcgatagc cgaataatcg agaatatcgt tgaggatggt cagcaacgat tcgccagagt
    17821 cggtaattgc ccgcaaatca tcacgctgag cgttaagtgc ggggttatct gccagtagct
    17881 gtgcggtacc gagaataccg tacagcggtg tgcggatctc atggctcatc gccgccagaa
    17941 acgctgattt tgcctggttg gctttttctg cttccgcccg tgcctgtcgg tgttctatca
    18001 ccagttcctg caattcagct gtacgcgctt tgacctgcgc cgccagctgt tcgcggtggc
    18061 gattcagtgc atgaacactg ctgcgaaacg catccatcag ccgcccgatg gtgtccagct
    18121 cccgcacgcc agcggtttcc ggaaaagggg agtcgatatc accgtccagt aagcgttgca
    18181 gcgcctgcgt ttgttcggca agtggacgcg tgactgagcg ataaaccacg cgccagagga
    18241 tcagaatcag tgcgcataga gaaactattc ccagcaataa caggctgtat tgcccacgtg
    18301 cactggcttt ttccagatgc gccagtccgt gctgattacg cagctcaata gtgtcgacca
    18361 gctgactgac ttcgctacta aactgcgcga actgggcgat gttattttgg gcgagagttt
    18421 gcaggcgatt gctgatttca ctgtcctgct ggaacagcgc cagcagatcg ctatattggc
    18481 taacggtagt taacgttgtt gcgacctgag tacgaacacc cggatcttca atgcgtattt
    18541 gccgacgttg cagaattttc accgcattat tgagctgctt ttccagcgtt ggcgcatttt
    18601 tctggatctg ttccagcccc agattcatca ccatttgctg cacccgcagg gcgctaaggc
    18661 gcagttcatt catctggtta acatactcaa gatcgatatc aatcagccga tcgagtgcac
    18721 tttctgcggc ctgacgctga tcttgttcaa tcaaatcgta aatcccggcc tgggtcgctc
    18781 cggcggaggt tgccgcatta ttcgcctgac cttgcgccag gcgtgcgatt tcatcagcgg
    18841 cggcgactat ctgctgactg agttgctgtt gttgctggcg cagttgcaaa cgctgcccca
    18901 ccagctcccc ttgttgacgt aacgaacggg aaatctcctg ctcctgttgt tcaatagcgg
    18961 tggtatcaaa accttgttcc cgtaacgctt gcagcaacgc attaatcttc aggctttgtg
    19021 cggtgagcat tcgcccctgc gcctgccaca tcttttcgtt atcggcactg gtcaggttct
    19081 gcgcggcgaa aagttcccag gcgctggctt cgctcaactg gcgcgccata ttcatggtag
    19141 gaatcaatgc ctgagtgttg tctttttcca cctggctgat aaagcgcagg ttgtaccatc
    19201 ccaccagggt actggtcagg gttaacagcg ccatcagggc aaagcccatc cagagtcttc
    19261 gggtcagggt taaattcacg gtcggtgcac tttaggtgaa aaaggttgag tcgcaaagca
    19321 gaatgcatct agcataaagc cttattattg atgaggctat catgcgcgta ctgctatttt
    19381 tacttctttc ccttttcatg ttatcggcat tttcggctga taacctgttg cgctggcatg
    19441 atgcgcagca ttacacggtg caagcctcta cgccgcttaa agccaaacgc gcatggaaac
    19501 tgtgcgcgct ttatcccagc ctgaaagatt catactggtt atcgttgaac tatggtatgc
    19561 aggaggctgc tcgccgctac ggtgtggatt taaaagtgct ggaggcaggc ggctacagcc
    19621 agttggctac gcagcaagca caaatcgacc agtgtaaaca gtggggcgca gaggccattt
    19681 tgctcggtag tagcacgacg tcatttcccg atctgcaaaa gcaggtagca aatctgccgg
    19741 tgatcgaact ggtgaatgct attgatgctc cccaggtgaa aagccgcgtt ggtgtgccct
    19801 ggtttcagat gggctatcaa ccagggcgat atctggtgca atgggcgcac ggtaaaccac
    19861 tgaacgtgct gttgatgccc ggccccgata acgccggggg cagtaaggag atggtagagg
    19921 gttttcgcgc agccattgcc ggaagcccgg tacgtattgt tgatattgcg ctcggtgata
    19981 acgatattga aatccagcgt aacctgctgc aggagatgct ggaacgccat ccagaaatcg
    20041 acgtcgttgc cggaacggcc attgcggcag aggcggcaat gggggaaggg cgcaacctga
    20101 aaacgccgct taccgtggtg tcgttttatc tttcacatca ggtgtatcgc gggttgaagc
    20161 ggggaagagt gattatggca gccagcgatc aaatggtctg gcagggtgaa ctggcggttg
    20221 agcaggctat caggcaatta caggggcaat cggtgtctga taatgtcagc ccaccgattt
    20281 tagtcatgac gccgaaaaat gccgaccgcg aacatatccg ccgctcgctg tcaccagggg
    20341 gatttcgtcc ggtttatttt tatcagcaca catcagcggc taagaaataa ccttcgccat
    20401 gttgtgtcac cagtaaatcc gcactgagtt tatggcgtaa acgacgaatt aacacatcga
    20461 cggtgcgcag gtcagggttt tccacccgac gcgcagagag catacgcagc agacgttcac
    20521 ggctgagaat ttcgcccgga ttagtcacaa atgccaccag catttcatac tctgcgcggg
    20581 tcagtttaat cggctcgcca tcccgctcca gcgtatggcg cgacacattc aggcaataac
    20641 cggcaaagcg atagcagttg tcctgagtgt gcggttgcgc ctgtcgcgcg aggtcgattc
    20701 gccagagcaa atttttcact cgtaccacca gttcgcgcag ctccagcggt ttggtgacgt
    20761 aatcgtctgc gcccatttcc agcccaacaa tacggtcaat ccgatcgctg cgtccggtaa
    20821 ccagaataat ccctaccgtt gagcgttctc gcagggcgcg ggttaacatc aggccatttt
    20881 catcgggcaa attgatatcc agcagaatca aatccaccgg ctgattctgc ataatttccc
    20941 gcagcccggc accgctcgcc gtaacggaaa cggtataccc ctcctgagtg aagtaagatt
    21001 gtaatcgcgc ctgggtaacc ggctcatctt caacaataac aatgtgatgt ggcatcagag
    21061 ggttttactc attctgttca tatctgttca tattctgccg taagccgttc atcctgacca
    21121 gtgccgctgt tcatatttgc tcattaagat cgcttcacta aaccataatt ttacaggggc
    21181 tattatgcgg aaactctgga acgcgctacg ccgacccagt gctcgttggt cggtactggc
    21241 gctggtcgct actgggattg tgattggcat tgcgctgatt gtattgccac acgttgggat
    21301 caaagtcacc agcacaaccg aattttgtgt cagttgccac agtatgcaac cggtgtatga
    21361 agaatataaa cagtcggtgc atttccagaa cgcctccggc gtgcgagctg aatgccatga
    21421 ctgtcatatc ccgccggata ttccaggcat ggtgaagcgc aaactggaag cgagcaacga
    21481 catttaccag acctttattg cccactccat tgatacacct gagaaatttg aagccaaacg
    21541 cgcggaactt gccgagcgtg aatgggcgcg aatgaaagaa aacaactcgg caacctgccg
    21601 ctcctgccat aactacgatg cgatggatca tgcgaagcag catcctgaag cggcgcgtca
    21661 gatgaaggtg gcagcgaaag ataatcaatc ctgcatcgac tgtcataaag gtattgccca
    21721 ccagttaccg gatatgagta gtggcttccg taagcagttc gatgagctgc gcgccagtgc
    21781 taatgatagt ggtgacacgc tgtactccat tgatattaag ccgatttatg cggcgaaagg
    21841 cgataaagaa gcgtctggtt ctctgctgcc tgcttcggaa gtgaaagtcc ttaaacgtga
    21901 cggcgactgg ctgcaaattg aaatcactgg ctggacggaa agtgcaggac gtcagcgtgt
    21961 actcacccaa ttcccaggta aacgcatctt tgttgcctcg attcgtggtg atgtgcagca
    22021 gcaggtgaaa acactggaga aaaccaccgt tgccgacacc aataccgagt ggagcaagtt
    22081 gcaggccact gcgtggatga agaaaggcga catggtgaac gatatcaaac cgatctgggc
    22141 ttatgcggat tcgctgtaca acggcacctg taaccagtgc cacggcgcac cggaaatatc
    22201 ccactttgac gctaacggtt ggatcggcac gctcaacggc atgattggct ttaccagcct
    22261 cgataaacgt gaagaacgca ctttgttgaa atatctgcaa atgaatgcgt ctgacaccgc
    22321 aggtaaagct cacggcgata agaaggaaga aaaataatga acaataacga tctctttcag
    22381 gcatcacgtc ggcgttttct ggcacaactc ggcggcttaa ccgtcgccgg gatgctaggg
    22441 ccgtcattgt taacgccgcg ccgtgcgact gcggcgcaag cggcgactga ggctgtcatc
    22501 tcgaaagagg gcattcttac cgggtcgcac tggggggcta tccgcgcgac ggtgaaggat
    22561 ggtcgctttg tggcagcaaa accgttcgaa ctggataaat atccgtcgaa aatgatcgcc
    22621 ggattgccgg atcatgtaca caacgcggcg cgtattcgtt acccgatggt acgcgtggac
    22681 tggctgcgta agcgccatct gagcgacacc tcccagcgcg gtgataaccg ttttgtgcgc
    22741 gtgagctggg atgaagccct cgacatgttc tatgaagaac tggaacgcgt acagaaaact
    22801 cacgggccga gtgccttgct gaccgccagt ggttggcaat cgacggggat gttccataac
    22861 gcttcgggga tgctggcgaa agctattgcc ttgcatggta atagcgttag tacgggcgga
    22921 gattactcta ccggtgctgc gcaggtgatc ctgccgcgcg tggttggctc aatggaagtg
    22981 tatgaacagc aaacctcctg gccgctggta ttgcagaaca gcaaaaccat tgtgctgtgg
    23041 ggctccgatt tgctgaaaaa ccagcaagcg aactggtggt gcccggatca cgatgtttat
    23101 gaatattacg agcagctaaa agcgaaagtc gccgccggtg aaattgaggt catcagcatc
    23161 gatccggttg tcacatccac ccatgagtat ctggggcgcg agcatgtgaa gcacattgcg
    23221 gttaacccgc aaactgacgt gccgctgcaa ctggcgctgg cgcatacgct gtacagtgaa
    23281 aacctgtacg acaaaaactt cctcgctaac tactgtgtag gttttgagca gttcctgccg
    23341 tatctgctgg gtgagaaaga cggtcagccg aaagatgccg catgggctga aaaactgacc
    23401 ggcattgatg ccgaaaccat tcgtgggctg gcacggcaga tggcggcgaa cagaacgcag
    23461 attattgctg gctggtgcgt acagcgtatg cagcacggtg aacagtgggc gtggatgatt
    23521 gtcgttctgg cggcgatgct ggggcaaatt ggcctgccag gtggtggttt tggttttggc
    23581 tggcactata acggcgcagg cacgccgggg cgtaaaggcg ttattttgag tggtttctcc
    23641 ggctctacgt cgattccgcc tgttcacgac aacagtgact acaaaggcta cagcagcacc
    23701 attccgattg cccgttttat cgatgcgatc ctcgaaccgg ggaaagtgat caactggaac
    23761 ggtaaatcgg taaaactgcc gccgctgaaa atgtgtattt ttgccggaac taacccgttc
    23821 catcgccatc agcagatcaa ccgcattatt gaaggctggc gcaagctgga aacggttatc
    23881 gccatagata accagtggac ctcaacctgc cgctttgccg atatcgtgct gcctgcgacc
    23941 acgcagtttg agcgtaacga tctcgaccag tacggcaacc actccaatcg tggcatcatc
    24001 gccatgaaac aggtcgtgcc gccgcagttc gaggcgcgca acgacttcga tattttccgc
    24061 gagctgtgcc gtcgctttaa tcgcgaagaa gcctttaccg aagggctgga cgaaatgggc
    24121 tggctgaaac gcatctggca ggaaggtgta caacagggca aaggacgcgg cgttcatctg
    24181 ccagcgtttg atgacttctg gaataacaaa gagtatgtcg agtttgacca tccgcagatg
    24241 tttgttcgcc accaggcatt ccgcgaagat ccggatctcg aaccgctggg cacgccgagt
    24301 ggcctgattg agatctactc gaaaaccatc gctgatatga actacgacga ttgtcagggg
    24361 catccgatgt ggtttgagaa aatcgaacgc tcccacggtg ggcctggctc gcagaagtat
    24421 ccgttgcatc tgcaatctgt gcatccggat ttccgacttc actcgcagtt atgtgagtcg
    24481 gaaactctgc gtcagcaata taccgtagcg ggtaaagagc cagtatttat tagcccgcag
    24541 gatgccagcg cgcgcggtat tcgtcacggt gatgtggtac gcgtctttaa cgttcgtggt
    24601 caggtgttgg ctggagcagt ggtttctgac cgttatgcac ccggcgtggc gcgaattcac
    24661 gaaggggcat ggtacgatcc ggacaaaggc ggcgagccag gggcgctgtg caaatacggt
    24721 aaccccaacg tcttgaccat cgacatcggt acttcgcagc tggcgcaggc gaccagtgcg
    24781 cacactacgc tggtggaaat tgagaagtac aacggaacag tggagcaggt gacggcgttt
    24841 aacggccccg tggagatggt ggcgcagtgc gagtatgttc ccgcgtcgca ggtgaaatca
    24901 tgaccacgct gacagcacaa cagattgcct gtgtttacgc ctggctggcg cagttgttct
    24961 cccgtgaact ggacgatgaa caactgacgc aaatcgccag tgcgcagatg gctgaatggt
    25021 tttcgttgct gaaaagcgaa ccgccgctca ctgcggcggt gaatgggctg gaaaacagta
    25081 ttgccactct gacagtacgt gacgatgccc gtctggaact ggccgctgac ttttgcggcc
    25141 tgtttctgat gaccgacaaa caagcggcgc tgccgtatgc atcggcctac aaacaggacg
    25201 agcaagagat taaacgcttg ttagttgagg cggggatgga aaccagcggc aatttcaacg
    25261 aaccggcaga tcatctggcg atctatctcg atttgttgag tcatctgcat ttttcgctgg
    25321 gagaggggac cgttcctgcg cgaagaatcg acggtttgcg gcaaaaaaca ctgacggctc
    25381 tgcgggaatg gttaccagag tttgctgcgc gttgtcgaca gtatgacagc tttggttttt
    25441 acgcggcact aagccagtta ttgctggtgt tagtggagtg tgactaccaa aaaagataat
    25501 gccgtttgta ggcctgataa gacgcggcag gcgtcgcatc aggcattagt gcgcagttgc
    25561 cggatgcggc gagaacgcct tatccggcct acgggaacag tgctttttgt gggcctgata
    25621 agatgcgtta agcgtcgcat caggcattat ggcgcagttg ccggatgcgg catgaacgcc
    25681 ttatccggcc tacgggaaca gtgctctttg taggcctgat aagacgcgtt aagcgtcgca
    25741 tcaggcaaca aactcacggg tgagccacaa accgggaaag ccgctggcgc agcaggcggt
    25801 tttcctgctt caggtgcgca atatcatcca ttaacgtcag cgccaccgcg atccccggcc
    25861 agtccagagc cagttcatga cgcaggcgta ccgcgcgttg caccacaatg gcggcatggt
    25921 cgtcaaatac ccaggttgtc tcctggatct cactcggttc aaccaccccc aaaccgacaa
    25981 tttcattcaa ctcctcttca gagatgccgg tatgcaggca aaattcggta atagtaaaag
    26041 tcaccgtaac attagccatt atgctttccc ccaatcttta cgtggatcaa aagacgactg
    26101 ggcgtctgcc agttgctgcc acagcgcggc agtgttttca tccggtttcg gcggcatcac
    26161 gattttcagt accgcataca gatcgccggt ctgttttttg ctcaccagac ctttgccttt
    26221 aacgcgcaat cgttgcccgg cctggctgcc aggcgggata gtcagcaaaa tgctttcttt
    26281 cagtgttgga acggtgactt tagtacccag cgccgcttcc cacgggctaa ccggcaccac
    26341 tatttccaga tcctggccga caatatcaaa cagcggatgt ggcgcaatgt gaatcaccag
    26401 ccacaggtcg ccatttggac cgccgttttc gcccggcgtc ccctggcctt tcaggcggat
    26461 gcgttgacca ttgccgacgc ccgccgggat cttcacattc agcgttttcg ggatttcctg
    26521 ttcgatcatg ccaaaggcgt tataaaccgg caggttatag ctgatggtac gcttatgctc
    26581 agtaagcgtt tcttcgagga aaaccgccac ttcgatttca atatcgtggc cgcgtgtggc
    26641 ggggcgttga cggctctggc gggcatgctg accgaaaatt gacgagaaga tatcgtcaaa
    26701 atcttcggcg ttaaaactct gaccgtcgcc atgatggaac tgacggttaa attgcggatc
    26761 gttgcgatgt tgccacatct gatcatactc agcgcgacgt tgttcatcac ttaacacttc
    26821 ccaggcttcg gcgacctctt tgaagcgggc ttcggcatcc ggttctttgc tgacatcagg
    26881 atggtatttg cgggccagtc gacgataggc ggtcttgatt gtcttgagat cgtccgtcgg
    26941 tttcacgccc atgatggcgt aataatcctt taattccata gcgttatctc gcgtaaatca
    27001 acacaaattg aaggaacccc tgtaaggtaa ctcctataag tgtagggtaa tcctcaaaat
    27061 ttcatatgcc aacacagaat atgttattga aatcatcgcg gaggggaggt tgcctcaagg
    27121 tgggaggaac atattttatt tacctcactc ctgaactttt ggctcatgct tacaagttaa
    27181 acaataagga aaggaatgca caactcgact atttaaatcg ttatgcaatt taatgataaa
    27241 gacatgtgct aaaaaccatt gctattagtc tcacattctt tttttattgg taatggtaac
    27301 gccgctgatt ttctttatag ctactgatgt aatcattagc ggcgtaaacg aggccggggc
    27361 taatcaggca tagtttgcgt caaaacttgc ctgtttttaa aggggaatat agaaaaacag
    27421 gcgttcaacg agccattttg cgaacctgtt cccggaaaaa tgacatattt ctgtcacact
    27481 ctttaattat tgataacaaa agaggtgtga ggaatgaaca aaacgctaat cgccgcaact
    27541 gtggcaggga tagttttact cgcttcaaat gctcaggcac aaaccgtacc ggaaggctat
    27601 cagctacagc aagtccttat gatgagccgt cataacttac gtgcgccgct ggcgaacaat
    27661 ggcagtgtgc tggagcagtc gacgccgaat aaatggcctg aatgggacgt ccccggtggg
    27721 cagctcacca ctaaaggggg cgtgctcgaa gtgtatatgg gccattatat gcgtgaatgg
    27781 ctggcagaac aggggatggt gaaatcaggg gaatgcccgc caccggacac cgtttatgcc
    27841 tatgccaata gtctgcaacg caccgttgcg accgcacagt tcttcattac cggcgcattc
    27901 ccggggtgtg atattcctgt tcatcaccag gaaaaaatgg gcactatgga ccccaccttt
    27961 aacccagtga tcaccgatga ttccgccgca ttcagtgaac aggcggtggc ggcaatggag
    28021 aaagagctca gcaaactcca gcttaccgac agctacaagc tactggaaca aatcgttaac
    28081 tataaagatt ctcctgcgtg taaagagaag caacaatgtt cgcttgtaga tggcaaaaat
    28141 acctttagcg cgaagtatca acaagaaccg ggcgtttccg ggccgctgaa agtcggcaac
    28201 tcgctggtag atgcgtttac tttgcaatac tacgaaggtt ttccgatgga tcaggtggcc
    28261 tggggagaaa tcaaatctga ccagcagtgg aaggtgttgt cgaagctgaa aaacggttac
    28321 caggacagcc tgtttacctc accggaagtg gcgcgcaatg ttgcgaaacc gctggtcagt
    28381 tatatcgaca aagccctggt cactgaacgc accagtgcac cgaaaatcac ggtattagtt
    28441 gggcacgact ccaacattgc ctctctgtta acggcgctgg atttcaaacc gtatcagttg
    28501 catgaccaga acgaacgcac gccgattggc ggcaaaatcg ttttccagcg ttggcatgac
    28561 agcaaagcca atcgcgattt gatgaaaatt gaatatgtgt accagagtgc ggaacagtta
    28621 cgtaatgccg atgcgttaac cctgcaggca cctgcgcagc gcgtgacgct ggaattaagc
    28681 ggttgcccga tagatgccaa cggtttttgc ccaatggata agtttgatcg cgtattgaat
    28741 gaagcggtga aataacagaa aactcccccg cgtgaagcgg gggagtcgct ggttaaacgt
    28801 ttttacgttc gatggtctgt tcgccccaaa aaagcgaatc tttatcggtc ttagcaaagg
    28861 ctttgactaa cacttcatcg ctaccttctt cccaaatctt ttccgccatt ttttcgtcgt
    28921 acccggcgac ttcgaaaatg gcctcggcta tttccggcga cgtattgcgc agagatgccc
    28981 attcaccgac gtgatgagct ttcgcttctt gagttggcat gcgtatcctc ctgttgaaga
    29041 ttagccgtta agtttaactg ccagacctgc gacatattcc ccttgataac gagcaataga
    29101 cagttcttcc tggctgggct ggcgtgaacc gtcaccgcct gcgatggtgg ttgcgccgta
    29161 cggcgtaccg ccgcgaacct gtgaaacgtc aaataattcc tgcgctgcgt agccaatggg
    29221 gacaatcacc atgccgtgat gcgcaagggt cgtccaggtg gatgtgatgg tttgttcctg
    29281 accgccgcca gtaccggtgg aactaaagac gctcgccagt tttccgtata gtgcgccgga
    29341 agcccacagg ccgcccgtct ggtcgaggaa ggtacgcatt tgaccggaca tgttaccaaa
    29401 gcgggtaggt gtaccaaaaa taatggcgtc gtaatcggcc agttcttgcg gggttgcaac
    29461 cggtgcagtt tgcgttttac cgcctgcttt ttcaaataat tgcggcggca tggtttccgg
    29521 tacacgctta acgacaactt ctgcgccatc cactttgctt gcaccctcag caactgcgcg
    29581 agccatcgtt tcaatatgtc cgtacatgga ataataaagc accagaactt tagccatttc
    29641 taaccactcc tcgtgttatc tctattccgt agcgattcgc taccacttat ttaaagataa
    29701 gacgtccttt tcagagtgca aatttcacaa ccacttattt gatttataac aactttcaca
    29761 agcacgtaat tttgtcgcaa aatgacacat ttttatctca tcgcgttttt ttaatcataa
    29821 gagcggctta tggataatta ttggagatga tatctattct cgctaagaag ctgttgcagg
    29881 atattaccaa acgcggatct gcccgcgtca gttcactaag cttagttcca cgtagcgaaa
    29941 atatggcagc cgccatacgc cgcgttaatt ctatgcaata tgatgtctat acccagacgg
    30001 aggtcagtaa tggcaaacca tcgaggcggt tccggcaatt ttgcagaaga ccgcgaaaga
    30061 gcatcagaag caggtaaaaa aggtggacag cacagcggag gtaatttcaa aaatgacccg
    30121 cagcgcgcat ctgaagcagg taaaaaaggt ggcaagagca gtcacggtaa aagcgacaac
    30181 tagccgggct aatcaatgac gcatgcattt ttgtctgtag ctcgtcaaaa cgccatcacc
    30241 gccggtagcc ccggcggttt tctgtttatg gcttctgatg aacaacttct ggcggtggaa
    30301 cgtcaaccaa ccttctgctt aacaacgcat tgagtaaaat cgcgccaaag gtggctgtac
    30361 cgatccctcc caacgtaaaa ccgcccagtg tgagagcaaa atcacccgca cccagcacta
    30421 aggttactgc gaccataatc aaattaccgt tctggcttaa atccacactg ttttgtaccc
    30481 agatccgtgc gcctgcgacg gcaatcagcc cgaacacaac aattgatgca ccaccaatca
    30541 ccgcagccgg aatagtatgg atcagcgcgc caaatttcgg cgaaaagccc aacagcatgg
    30601 cgatgacggc agcggcaaca aacaccagcg tcgagtagac ttttgtcacg gccatcacac
    30661 cgatattctc ggcgtaggtg gttatgccgc taccgccgac agagccagaa agcatcgtcg
    30721 ccagcccatc gcctacgaat gcccgcccca tatacgggtc catattgcgt ccggtcatcc
    30781 cggcgaccgc cttgagatga cctaagtttt ccgccaccag aatcaccgcc acgggcgcaa
    30841 tcagcatcat tgcctgacca ttaaaagcag gagtggaaaa atgtggcaga ccgaaccagg
    30901 cagcatggct gacgagagta aaatcgacgg cttttcccag ccctaaaacg ttggtcatca
    30961 cgccatacag cagacaggcg acaattaatc ctacgagaat caataatcgc tggatcatgc
    31021 cacgggtaaa tacggccacc agcccaatac acagcaccgt cattaccgcc atccagctat
    31081 caaaggccga agccgataca tttttcactg cgataggcgc taagttcagg ccaatcgcca
    31141 tcactaccgc acccgtcaca accggcggca ttagtcgttc aatccagcgc gtaccgattt
    31201 tcatcaccac caggccaatg acggtataaa ccagcccaca ggcgataatc ccgcccagcg
    31261 cgatgctgat gttcggatta attccctgac cattaaagcc cgtcgcggca atcaccacgc
    31321 cgacaaaagc cgcgctggaa ccgagataac tgggcacgcg cccgccggta ataaagaaaa
    31381 acagtaacgt gccgatcccc gacattaaaa tggaaagatt gggatccagc cccatcagaa
    31441 tcggcattaa caccgtcgcg ccaaacatcg ccaccgcgtg ttgaacgccc attactgccg
    31501 tttgagcaaa cggcaatcgt tcgtccggcg cgaccacgcc gctctctgta gaggtcgatt
    31561 ttaactgcca gtgaggaaaa ccgaacattg ccatcagctg tctccttaag gaggttaaca
    31621 agcagggcgc atcagcgcgt gataactgcg atcgaaccac accagcccgt agggtgtggc
    31681 gtgacgatga atcgcttcga tggcgcaaaa caaaatgtcg tgggtgccga cgctcaccac
    31741 ctggctgata cggcagtcaa acgaaaccag tgccttttcc agttgcgggc atccggtcac
    31801 accagtctgc cagcgggcgg cggcaaagcg gtgttccatg ggcgttttgc cgccaaaaag
    31861 gtttgaaagc ggctcctgcc cggcgctaag tgtatttaca cacagcgttc gattttcatt
    31921 gaataccggc cagacggacg ccccacgatt caggcacacc agtaatgtgg gcggcgtgtc
    31981 ggtcacgctg cagacggcgc tggcggtgaa cccggcgcgc ccggctggac cgtccgtagt
    32041 gatgatattg accgccgcgc ccatacagga catcgcatcg cgaaaggttt gttgatcgac
    32101 aatgttcatt gtttgctcct tacaacagcc cgcaggcttc ttcaaaggac agacgtggca
    32161 ggcgcgcaaa aagtttgctg gaatcgccat agccgatatt aatcagcaga tcgctcttca
    32221 gcgtgctgcc cgcaaaaaag gcgtcgtcaa cgtattgacg gtcaaagccc gacatcgggc
    32281 cggtatccag ccccagcgca cggcaggcga cgatcagata ggccgcctgc atggaactgt
    32341 tgcgaaacgc tgtttcttcg gcaagttgtg gactggaggt aaaccaactg cgggcatcgc
    32401 cgtgaggaaa cagctgtggt aaccgttcat aaaattcact gtcccaggcg acgatagcgg
    32461 tgacgggcgc ggtcagggtt ttttgcagat tgccgctgga aagcgccggg cgcagacgtt
    32521 cttttccttc tgcagtgcgg ataaacacga tccgggccgg agaacagtta gctgatgtcg
    32581 gcccccattt catcagggca taaatctccc gtaacgtctc atcgctgacg gggggctccc
    32641 gccagccgtt gtgagtgcgg gcatcggtga acagggtgct aagcgcacct gggctaacgg
    32701 cttcgttcat agcaattcct tacagagcgg cttcacggtg atgtaacagg ctggcaagcc
    32761 cgttgagtaa cagagcatta aacgtttcgg gatcggtcac gttgcaggcg tgtccgccat
    32821 agcgcatcac cattttctgg ctatcgggca gggcggcatg aagttcactg gaacatgctg
    32881 ttggcaccag cagatcgtca ctggcgcaga tgatttgcac cgggcagcgg atacgatccg
    32941 catggtgact aaagtcagcg cgtttgaggg cgttaagtcg acgcagtaaa ttatttttgc
    33001 cctgaaaatg cgccagtgcc agcgcatctt ctgcctcaag gcgaggtgcg cgggccgcca
    33061 tccagtcggc gggatagagg aacaacggct gcgcttccac ccatgcctgc gcgccgccgc
    33121 tatgtagtaa ctgttcgcga acctgaaaac agcggcgcgt atgggtgttt attcgtagcc
    33181 agccgttaac gctgaccagc acagttaccg acgcgggata atccagcgcc agctgcattc
    33241 ccaccagcgc accgagcgca tggccgacca ccgcgtaacg ctcaatccct gcggctacca
    33301 gcgcctgatg cagttccgct gccatctggg cgatactgta atcttctgcc agcgtgtcgg
    33361 gattattgcc ggtgccgcgc tggtcgtaac agactacctg atactcctga tccagcaccg
    33421 ccagttgcgg taaccagtaa ctgccgctac cgccaagacc cgaaatcaac accactacgg
    33481 gcgcatcagc ataaggggga ggtgagagtg aaagtttcat cgcggcctca cttggcgata
    33541 tgcgcaattg tggcgatttc caccagcgcg tcaggtttta ccagtccgca ctgaatgcag
    33601 aatcgcgccg gtttgtcacc cgggaaaaac tcggcgtaga tttcgttaat cgcggcgtaa
    33661 tttttccagt cggtaataaa gatgctgttg aaagtcacat ccgccatcgt gccacccgcc
    33721 gtctcgatga ccttgcggat ggtttccaga acgtggcggg tttgcgcctt tgggtcatcg
    33781 gcaaacagca cgttgttatg ttgatcaaaa gccagcgtac cggagacata caccacgcca
    33841 tcagccagcg tgccgggaac gaagggggcc agcggtgcgc tgctgccagc aggaataatt
    33901 acggattttg gcatcgttaa actccttaag cgatacgagc aaaggacgtg gaagaaagcg
    33961 cgtcgcagaa cgtttcgacg tcgctgaccc agccaaaaaa ggtttcgata ttgaacaacg
    34021 cagctttctg tgcaaattcc ggccccgcct ggtgtgttgc gtcttccagc accacgccga
    34081 aatactccag aaaaaagccg tcgcgtagcg tcgattcgac gcagacgttg gtagcgatac
    34141 cggtgaaaac cagatggcgt attccacggc tgcgcaaaat gctgtccagc ggcgtattga
    34201 agaaaccgct gtagcgcggc ttcggcagca caatatcgcc aggctgcggc accagttcat
    34261 ccaccagttg ataatcccag gagcctttcg ccagcaattt cccctgcagc tgcggctgct
    34321 tacgcatggt tttcagggcg ttcgatttat gaaaattcgg tgagccgggg ccgccagcct
    34381 cgacatactg ttcatcccag ccattttgaa accagatgat cagcatccct gccgctcgcg
    34441 ctgcggtcac ggcggtttga atgttggcaa tgaccgggcg agtggttgag acatcaaacc
    34501 cggcgagatc taagtagccg cctggcgtgg cataagcgtt ttgcatatcc accacgatca
    34561 gcgcactttg ctgcggatcg aaggtaatgg cttccggtcg agcggttaag gtcgtcatca
    34621 tgccacctcc tgagtcagcg cagggagatg ggcgcggcac tgcatcagtg gttgaatgcg
    34681 ctcgccgaag gtttcgattc ccgacagaaa atcatcgaag gtcaacagca cgccttcggc
    34741 accaggcacg tttgcgactt catctaacat gcgcgcgaca ctggcgtaag aaccgactaa
    34801 cgtccccata ttgatgttta ccgccgaagt gggatcggcc atctgacgga cgttggtgtc
    34861 agtacctgag cgggtatctt tctgactttg ttcggttagc cagcttaacg cctcttcatc
    34921 cgcgcccgct ttgtagtgtt cccattttgc acgagcggca tcgtcggttt cgtcggcaat
    34981 caccataaac aacacataag agccaacgtc gcgtccggtt tgctctgcgg cctgtttcat
    35041 ccgcgcagcg gtcggggcga aagccgtggg tgtatttacg cctttgccga aacagaagtt
    35101 aaaatcggca taccgggcgg agaacgccat gccagcgtcg ctttgcccgg cgcagatcac
    35161 tttcatgggg acactcggtt gcggactgac gcgacaatca tccatagtga aaaaatcgcc
    35221 tttaaaatcg cttttccccg agccccacag gtcgcgcagc acctgaacat attcggtgag
    35281 atagtcgtaa cgacgggaga aatagtcatc gccaggccag atccccatct gctcatactc
    35341 gggcttctgc cagccagtca cgaggttgac gccaaaacgc ccgccagaga tggagtcgat
    35401 ggttgcggcc atacgggcga cgattgccgg aggtaacgtt aaggtggcag ctgtcgcgta
    35461 tatctgaatg cgcgaggtca cggctgccag ccccgccatc aaggtgaacg actcaaggtt
    35521 gtgatcccag aactcagttt tgccgccaaa gccacgcagt ttgatcatcg acagggcgaa
    35581 atcgaaatgg tagtgctccg ctttttgcac aatggcttta ttcagttcaa atgtcggcat
    35641 gtactgcggc gcgtgggtcg aaatgagcca gccgttgttg ccaataggta cgaatacgcc
    35701 aattttcatc atcaacctct cttcgtctcg taaagtgaaa gtcagacggg gcgctgcatc
    35761 ctgcatatcc ttttcagccg cgtaatggct tgtttgcaaa gcggatgcca gtttttaaaa
    35821 agtaaatgtt attaatgtgt taatgttaag ttgtctaaaa tgcctgaaaa aaagtggact
    35881 aaacggtcaa aacagttgca cataaaacat gcattcgtgc gcgatgagag tgcagaaggt
    35941 cgaggccggg cgggggtttt gctatcctgt tgccaatcta caagagggga gagcgcatga
    36001 cgcaaggcgc agtgaaaaca acgggtaaac gttcgcgcgc agtgagcgcg aagaaaaaag
    36061 cgattcttag cgcagcactg gacacttttt cacaattcgg ttttcacggc acaaggctgg
    36121 agcagatcgc ggagttggcg ggtgtttcaa aaaccaatct gctgtattac tttccgtcaa
    36181 aagaggcgct gtatatagcc gtactgcggc agattctcga tatctggctg gcaccgttaa
    36241 aagcgtttcg agaagatttc gccccgctgg cggcgatcaa agagtacatc cgtctgaagc
    36301 tggaagtttc ccgcgattat ccgcaggctt cgcgcctgtt ctgtatggag atgctggcag
    36361 gtgcaccgct gttaatggat gaactgacgg gcgatttgaa ggcattaatt gatgagaaat
    36421 cggcgctgat tgccggttgg gttaaaagcg gcaaactcgc gccgattgat ccgcagcatt
    36481 tgatttttat gatttgggct tccactcaac attacgccga ttttgcccct caggtggagg
    36541 cggtgacagg cgcgacgttg cgcgatgagg tatttttcaa tcaaacggtt gaaaacgtgc
    36601 agcggattat tattgagggg attcgaccac gctaatggtg ccggaggaag gtgtaacatc
    36661 ctccggctac ctgtttaacc gatcgtcatt aaactggcgt taccgccagc ggcagcggta
    36721 ttcacactca gcgaacgctc gatatacagc cgttccagaa ggatattgct ttcgccacgg
    36781 gcaaaaccct gcaccgaaac aattgcacca tcccgcgcgg caacggcttc acacaatgcg
    36841 cgaagctgat ccgaatcacc gtggaagatc accgcatcaa atggttgagc ggttatattt
    36901 tccgctttcg ccagttgaat acgttcgctg actgccgatg gcaatgcctt cactaactga
    36961 cgatgcagcg cattatccgg ccacagcacc tggctaccca ccgccagcac ggcggcgagc
    37021 tgagtcagcg catcctgttc atcatccgca atacacaaca cgcgctcacg cggcagcagc
    37081 gtccaggtgt tgcgttcacc cgtcggccct ggcaacaatc gttgtgttcc tgcctgcgcc
    37141 aactcgccat attgcgtaca taacgcctgc aattctggac ggtttgccgc ccattcccgc
    37201 agtgcattta gcggctgagt caatgcggct ttcaactgcg catcgaccgg atactccgca
    37261 tcctgacgcg cgagcgtcac cgccagcgca ctttccgggc gattcgccag cagacggtag
    37321 agatagagcg gaccgcctgc tttcggcccg gtgccggaca atccttcgcc gccgaacggt
    37381 tgcaccccaa ccactgcgcc caccatatta cggttaacgt acagattacc gacatgcgcc
    37441 gagccagtga cctgggcgat ggtttcatca atgcgcgtat ggacgccaag cgtcagacca
    37501 taaccggaag cgttaatctg ctcgatcagc tctgggagct ggttacggtt gtaacgcacc
    37561 acatgcaaca ccggaccaaa gacctctttt tgtaattcgg caaagtcatc cagttcgatc
    37621 agcgtcggag cgacaaaggt gccgctttgc cattcacggg catcttcgct gttttcccgc
    37681 accgcctgga acacctgacg gcctttacta cgcatggtct gaatatgacg ctcgatattg
    37741 gctttcgctt cgctatcaat caccggaccg atatcggtgg tcaggcgacc cggattaccc
    37801 atccggcatt cggccattgc gccgcgcagc attttcagcg tgtggtcggc aatctcatct
    37861 tgcaggcaca gcacgcgcag tgccgaacaa cgctgacctg cactgtcgaa cgccgaggcc
    37921 agcacatcta ttacgacttg ttcggtcagt gctgaagaat cgacaatcat cgcgttcatg
    37981 ccgccggttt cagcgatgag cggaataggg cgaccctgag cgtccaggcg gctggcgata
    38041 ttgcgctgca gtaacgtagc gacttcggtt gaaccggtaa acatcacccc gcgcacgcga
    38101 tcatcacccg tcagttgcgc gcccacggtt tcaccccgac ctggcagcaa ttgcaccacg
    38161 cctggcggta cacccgcttc cagcaaaatg gcgatccctt gcgcggcaat cagcggcgtt
    38221 tgttctgccg gttttgccag cacgctgtta cctgccgcca gtgcggcagc gatctgtccg
    38281 gtgaaaatag ccagcgggaa gttccacgga ctgatacata ccacaggccc taatggacgg
    38341 tgggtttcgt tagcgaaatc atcccgcacc tgtccggcat agtagtggag aaaatcgacc
    38401 gcttcgcgca cttcggcaat ggcgttactg aaggtttttc cggcttcacg caccagaata
    38461 ccaatcagtt gctgcatctg gctttccatt agcacggcag cgcgatgcaa aattgccgcg
    38521 cgttcagccg gaggagtggc aaaccagatt ggcgcgttat taaccgcact ttccagcgcc
    38581 tgttctactt cacgcggcgt ggcttcacgc acatagccca caatatcttt cggttccgca
    38641 gggttaataa cgggcgacat ctcacctgcc gctaccggtt gttccagcat tggcaaggac
    38701 tgccattttt gcagtgcact attgagcagg gctgaagaga gcgaggccag acggtgttcg
    38761 ttagcgagat ccagccctgc cgagttgtcg cgcccgtgac cgtaaagatc gcgcggcagg
    38821 ggaattttcg gatgcggtaa tccagtttgc ccttcctgtt gcgccagttt ttctacagca
    38881 gtgactggat cggcgaccag ttcatccagt ggcaaagagg tgtcggcaat acggttaaca
    38941 aacgaggtgt tagcaccgtt ttccagcagg cggcgcacca gatatgccag cagcgtttca
    39001 tgtgtaccga ccggagcata aatacgacac ggacggttaa gtttgccgtc ggcaactttc
    39061 ccggtgacct gctcatacag tggctcgccc ataccatgca ggcactggaa ctcgtactga
    39121 cccgggtagt agttttgacc cgccagttga taaattgccg ccagcgtatg ggcgttgtgc
    39181 gtcgcgaact gcgggtagat tagatttggc accgccagca gttttttcgc acaggcaaga
    39241 taagaaacgt cggtatacac cttgcgggta taaaccggat aaccttcaag gccgtccatc
    39301 tgtgcacgct tgatttcact atcccagtac gcgcctttca ccaggcgaat catcagacgg
    39361 cgacggctgc gggtggcgag atcaatcagg taatcgatca ccaacgggca gcgtttttga
    39421 taagcctgaa taacaaaacc gatgccgttc cagcctgcca gttcaggctc gaaacagagt
    39481 ttttccagca gatcgaggga gatctccagg cgatcggcct cttcggcatc gatgttaata
    39541 ccaatatcgt actgacgcgc cagcagggtg agtgatttca gacgcgggta aagctcttcc
    39601 attacccggt catactgggc gcggctataa cgcggatgta gcgccgacag cttgattgaa
    39661 atgcccggac cttcatagat gccacgaccg ttagacgctt taccaatggc gtgaatcgcc
    39721 tgctgatagg aaaccatata cgcctgtgcg tctgcggcag tcagtgcggc ttcgcccagc
    39781 atatcgtaag agtaacggaa acctttctct tccagtttgc gggcattggc taacgcttcc
    39841 gcgatggttt cgcccgtgac gaactgctcg cccatcaggc gcatcgccat atccacacct
    39901 ttgcggatca gcggttcacc gctcttaccg ataatgcggt tcagcgagcg ggagaggctg
    39961 gcttcgttat gggtggaaac cagtttgccg gtaaacagca gcccccaggt agcggcatta
    40021 acaaacaggg acgggctacg accaatgtgt gactgccagt taccgttact gattttgtcg
    40081 cgaattaacg cgtcacgggt ggctttgtcg ggaatacgca acaacgcttc cgccagacac
    40141 atcagcgcca cgccttcctg cgatgacaac gaaaactcct gcaataaccc ctgaaccata
    40201 cctgcgcgac cactggcgtt tttttgatta cgcagtttat cggccagttg atacgccagt
    40261 ttgtgcgcct gttcagcaac tggctgcggc aggcgagctt gttccagcag catagaaacc
    40321 gcttcggttt ccgggcggcg ataggccgcg gtgatcgcgg cgcgggaaac cgactggggc
    40381 aatatttgtt cggcaaagtc gaggaaaggc tggtgtggtt cctctgccgg agtcggtgct
    40441 tcatcgctct cattggccgc gccagaaagc agcgcaggta gctccgggag agtatcgctg
    40501 ttttccagtt gttcaagata agaaaaaatc gcctgtttaa ttaaccagtg tggtgtgcga
    40561 tcgatacgtg tcgcagcaga cttaatacgc tcacgcgtcg cgtcgtccag cttaaccccc
    40621 atggtggtgg ttcccatgcc attactcctg ttgttctgaa aggtgcaact taacgttatc
    40681 gtgaaatatc catgatgttg caactttgtg caaccatgtt aaatgtgacc tgcgtagcaa
    40741 gcttaaaaat gaatgaaatg ttaataaacg aaatcgatat gacagggatt aaaaaaataa
    40801 ctcagacttt ttctctgcgg cagttaacat ttttgaaagg tgcaaccgca aaaaatgtga
    40861 gagagtgcaa cctgatgaaa aatagtgtcg ctgagcacta aaatttaatg taaatggtgt
    40921 gttaaatcga ttgtgaataa ccagcgcttc cggcaggata cggtcgccct ggtaaaacat
    40981 aaactctgtt accccgttcc ggtggcagat ataacggcaa gtttcgacat tgccgataat
    41041 aattttttgg agactttaga tggctattag cacaccgatg ttggtgacat tttgtgtcta
    41101 tatctttggc atgatattga ttgggtttat cgcctggcga tcaacgaaaa acttcgatga
    41161 ctatattctg ggcggtcgta gtcttgggcc attcgtgacg gcattatcgg cgggtgcgtc
    41221 ggatatgagc ggctggctgt taatggggtt gcctggcgct gtttttcttt ccgggatttc
    41281 cgaaagctgg atcgccattg gcctgacatt aggcgcgtgg atcaactgga agctggtggc
    41341 cgggcggttg cgtgtgcata ccgaatacaa caataacgcc ttaacattgc cggattattt
    41401 caccgggcgc tttgaagata aaagccgcat tttgcgcatt atctccgcgc tggttatttt
    41461 gctgttcttc accatttatt gcgcttcggg cattgtggca ggcgcgcgtc tgtttgaaag
    41521 tacctttggc atgagctacg aaacggctct gtgggcgggg gctgcggcga cgatccttta
    41581 cacctttatt ggcggtttcc tcgcggtgag ctggactgac actgtacagg ccagtctgat
    41641 gatttttgcc ctgatcctga cgccggttat cgtgatcatt agcgtcggtg gctttggcga
    41701 ctcgctggaa gtgattaaac aaaagagcat agaaaacgtg gatatgctca aagggttgaa
    41761 ttttgtcgcc attatctcgc tgatgggttg ggggctgggt tacttcgggc agccgcacat
    41821 cctggcgcgt tttatggcgg cagattctca ccacagcatt gtccatgcgc gtcgtattag
    41881 tatgacctgg atgatcctct gcctggcagg ggcggtggct gttggtttct ttggtatcgc
    41941 ttactttaac gggcacccgg cggtagcagg tgcggtaaac cagaacgctg aacgcgtgtt
    42001 tattgaactg gcgcaaattc tgtttaaccc gtggattgcc gggattttgc tgtcggcgat
    42061 tctggcggcg gtaatgtcaa cgttaagttg ccagctgctg gtatgttcca gtgcaattac
    42121 cgaagatcta tacaaagcat ttctgcgtaa acaggccagc cagaaagagc tggtgtgggt
    42181 agggcgtgtg atggtgctgg tggtggcgct ggtggcgatt gcgctggcgg caaacccgga
    42241 aaaccgcgtg ctgggcttag tgagctacgc gtgggcaggc tttggcgcgg cgtttggtcc
    42301 ggtggtgctg ttctcggtga tgtggtcacg catgacgcgc aacggtgcgc tggcggggat
    42361 gatcatcggt gcgctgacgg ttatcgtctg gaaacagttc ggctggctgg gtctgtacga
    42421 aattattccg ggctttatct tcggcagtat cgggattgta gtgtttagtt tgctgggtaa
    42481 agcgccgtca gcggcgatgc aaaaacgctt tgccgaggcc gatgcgcact atcattcggc
    42541 tccgccgtca cggttgcagg aagggtaagg gaaggcgttt taccgaaaat ttcacgtcat
    42601 aacaggaagc aggcagaggg caatcagtga ttgccctctg cgttttttct caaaatattt
    42661 ttacatcttc tcatcattat cactacattt cacttgtatt tttcttcatc gtaatgataa
    42721 tcgctatcac tgcgatttac ttttctttgc atagattgac taagaaaaac gtttaagggt
    42781 gggtggcatg tttgttccgt ttctcattat gttgcgcgaa ggacttgaag ccgcgctgat
    42841 tgtcagtttg attgccagct atcttaagcg tacccagcga ggccgatgga ttggtgtaat
    42901 gtggattggc gtgttgcttg ccgctgcgtt gtgcctgggc ttgggtatct tcattaacga
    42961 aaccaccggc gaatttccgc aaaaagaaca ggaactgttt gaaggcattg tggcggtgat
    43021 cgccgtggtg atccttacct ggatggtttt ctggatgcgc aaagtgtcgc gcaacgtcaa
    43081 agtacaactg gagcaggcgg tcgatagcgc attgcagcgt ggaaatcatc atggctgggc
    43141 gctggtgatg atggtctttt ttgccgttgc aagggaaggg ctggagtcgg tctttttcct
    43201 gctggcggca tttcaacaag atgtcggggt ctggccgccg ctgggtgcaa tgctcggtct
    43261 tgctacagcc gtggtgcttg gcttcctgct ctactggggc ggtattcgcc tcaatcttgg
    43321 tgcatttttt aaatggacca gtctgtttat tctcttcgtc gccgcagggc tggcagctgg
    43381 tgccattcgc gcatttcatg aagccggatt gtggaaccac tttcaggaaa tcgccttcga
    43441 tatgagcgcg gtgctctcaa ctcactcgct gtttggcaca ctgatggaag ggatttttgg
    43501 ctatcaggaa gcaccgagcg tcagcgaagt cgccgtctgg tttatttatc tcatcccggc
    43561 gctggtggca tttgttctgc caccacgcgc aggggcgact gcgtcccgct ccgtgtagca
    43621 aatacgacgc aaactcttgc ttagttacaa catactttaa agggatagtc tcgtcatgac
    43681 cattaacttc cgtcgtaacg cattgcagtt gagcgtggct gcgctgttct cttctgcttt
    43741 tatggctaac gccgccgata ttccgcaagt caaagtcact gtgacagata agcagtgcga
    43801 gccgatgacc attacggtta acgccgggaa aacacagttc attattcaga accacagcca
    43861 gaaggcgctg gagtgggaga tcctcaaagg cgtgatggtg gtggaagagc gggaaaatat
    43921 cgcccctggt tttagccaga aaatgacggc gaatttacag cctggcgaat acgatatgac
    43981 ctgcggtctg ctgactaacc cgaaagggaa gttgatcgtc aaaggtgaag caacggcgga
    44041 tgcggcgcaa agtgatgcgc tgttaagtct tggtggtgca attactgcat ataaagcgta
    44101 tgtcatggcg gaaaccacgc aactggtgac cgacaccaaa gcctttaccg acgctattaa
    44161 agcaggcgat atcgaaaaag cgaaagcact gtatgcgccg acgcggcagc actatgagcg
    44221 catcgaaccg attgctgaac tgttctccga tctggatggc agcattgacg cccgtgaaga
    44281 tgattacgag caaaaagccg ccgatccaaa attcaccggt ttccaccgtc tggaaaaagc
    44341 attgtttggc gataacacca ccaaagggat ggataagtat gctgaccagc tttataccga
    44401 tgtggtcgat ttgcaaaaac gcatcagtga actggctttc cctccttcaa aagtggtcgg
    44461 cggtgcagcc ggactgattg aggaagtggc ggccagcaaa atcagcggtg aagaagatcg
    44521 ctacagccac accgatctgt gggatttcca ggctaacgtt gaaggctcgc agaaaattgt
    44581 cgatctgctg cgtccacaac tacaaaaagc gaatccggaa ctgttggcaa aagtcgatgc
    44641 caacttcaaa aaggtcgata ccattctggc gaaataccgc actaaagagg gttttgaaaa
    44701 ttacgacaaa ctgaccgatg ccgaccggaa cgcactgaaa ggaccgatta ctgcgctggc
    44761 ggaagatctg gcgcaacttc gtggtgtgct ggggctggac taagcgttat gcagtatgaa
    44821 gatgaaaacg gcgtgaatga accgtcacgc cgacgtttac tgaaagggat tggtgcgctg
    44881 gcgctggcgg gaagttgtcc ggttgctcat gcacaaaaaa cgcaaagtgc gccgggtacg
    44941 ctttcaccgg atgcacgcaa tgagaaacag ccgttttatg gtgagcatca ggcagggatc
    45001 ctgacaccac aacaggctgc aatgatgctg gtggcgtttg atgtgcttgc cagcgataaa
    45061 gccgatcttg aacggttgtt tcgcttgttg actcagcgtt ttgcttttct gactcagggc
    45121 ggagcggcac cagaaacgcc aaatccgcgc ctgccaccac tcgattccgg cattcttggc
    45181 ggctacattg cgcccgataa tctcaccatc acgttatcgg tgggtcactc attgtttgat
    45241 gagcgctttg gccttgcacc acagatgcca aaaaagttgc agaagatgac gcgtttcccc
    45301 aacgactcgc tggatgcggc gttatgtcat ggtgatgtgt tgctacaaat ttgcgccaac
    45361 acccaggaca cggtgatcca tgcgctgcgc gatatcatca aacacacgcc ggatttgctc
    45421 agcgtgcgct ggaagcggga agggtttatt tccgaccacg cagcgcgtag taaaggcaaa
    45481 gagacgccga taaatttgct gggctttaaa gacggcaccg ccaatcccga tagccagaat
    45541 gcgaagttga tgcaaaaagt ggtgtgggtg acggcagatc agcaggagcc tgcgtggaca
    45601 atcggtggca gctatcaggc agtacgcttg attcagtttc gagtggaatt ttgggacaga
    45661 acgccgctga aagagcagca gacgatattt ggtcgtgata agcaaaccgg tgcgccgctg
    45721 ggcatgcagc atgagcatga tgttcccgat tacgctagcg atccggaagg gaaggtgatt
    45781 gcgctggaca gccatatccg gctggcgaat ccccgcacgg cggagagcga gtccagcctg
    45841 atgctgcgtc gtggctacag ttattcgttg ggcgtcacca attccgggca actcgatatg
    45901 gggctactgt ttgtctgcta ccaacacgat ctggaaaagg gcttcctgac agtacaaaaa
    45961 aggttgaatg gcgaggcgct ggaggaatat gtcaaaccta tcggtggcgg ctatttcttt
    46021 gcgctgccgg gtgtgaagga cgcgaacgat tatctcggaa gcgcattatt gcgggtttag
    46081 tgtttttagg cgtataaggc gtttacgccg caccaggcgt tcgtgcgctg atgcgacgct
    46141 tgcgcgtctt atcatgccta caatcggtgc gggtcggtag gccggataag gcgttcacgc
    46201 cgcatccggc aagcttttac cagtaccgcg aatcctgctg aaagcacaca gcttttttca
    46261 tcactgtcat cactctgtca tctttccagt agaaactaat gtcactgaaa tggtgtttta
    46321 tggttaaata taagtgaata tattgttgca atgaatgcga aatctgttgt acttattaag
    46381 tagcagcgga agttcccggc agtgatagtc agtcactatg gagatcgcgg atggtaacgt
    46441 cctgtactgg acatgtttta gagaatcagc gcgccaccac tcgcggagat ttctcctccg
    46501 gtagtcatct cgtcactttg tattttcaac ctcatccttt cttttcatgt gttaccgacg
    46561 ccgtaaacgg tgcgcgtagc cgtttttcgg cattttatcc aaaagcaaac tatggcttac
    46621 aaggaagcca accctctgat gttcgtgcgc ataatcgcgc tgccaacggc gcgtgtgatg
    46681 aatacaaaca actcaaggtg ctctccatgg gaagacaaaa agcagtgatc aaagctcgtc
    46741 gcgaggcaaa acgtgtgctg agacgggatt cgcgcagcca taagcagcgt gaagaagaat
    46801 cggtcacctc gcttgtgcaa atgagcggcg tagaagccat tggtatggcc cgcgacagtc
    46861 gcgatacctc gcccatcctc gcgcgaaatg aagcgcaatt gcactatctg aaggctattg
    46921 agagtaagca gctgatattt gccacgggtg aagccgggtg cggaaaaacc tggatcagcg
    46981 cagcaaaagc ggcagaggcc ctgatacata aggatgttga caggattatc gtcacccgtc
    47041 cagttctgca agccgatgaa gatcttggtt tcttacctgg tgatatcgca gaaaagtttg
    47101 ctccctattt tcgcccggtc tacgacgtgc tggtccgtcg cttaggggct tcctttatgc
    47161 agtactgcct gcgaccggaa atagggaagg tggaaattgc gccgttcgcc tatatgcgtg
    47221 gacgtacctt tgaaaatgca gtcgtcattc ttgacgaggc gcagaatgtg actgccgcgc
    47281 aaatgaaaat gtttttaacc cgcctcgggg agaacgtgac ggttatcgtc aacggtgata
    47341 tcacgcaatg cgatttgcct cgcggcgtgc gctccggatt aagtgacgcg ctggaacgtt
    47401 tcgaagagga tgaaatggtc gggatcgtcc gattcggtaa agaggattgc gtacgttcgg
    47461 cactttgcca acgtacgcta catgcataca gttaagtgta ttatcggtgc agagcccggg
    47521 cgaaccgggc tttgttttgg gtgtttatac ccggactagc gttttttctg aaaccaccat
    47581 ttttatttgt ccctggctgg tgaaatgtac gctcatcctg tggctttttt gtagttgctg
    47641 ataaagctca tcaggtattg ctaagctctc tgcatattct tgcggcgtat attggtatgc
    47701 tgcatgatgc tgctgttttt gaaaacgcag cttattgtac aacgcccaga cgattaacac
    47761 gacggcattc accactgcca gcaaaaaata gaactgaagt cggcttctgg cctcgctttg
    47821 ccagaaataa cccgtcagca gatcaatggc gaataagaat atgaataaag caaataatgt
    47881 ccacaagata gttgtggcaa cataatcaac cagtaaacgt actggcgatt gtcgggtcgt
    47941 aataattaga ttgttcatat taacctctca gaatcccacg atcgggactt acccaacgag
    48001 cgcgttgttt tttaggcatc aacatgactc gtgtaaatga taccaacgtc gttgccaggc
    48061 ttagcatcca gaaaataacc gggaaccaaa taatccagaa aagcgatgaa gccagattgt
    48121 gctcatagcg attctcaatc accagactga caataaattg cagtaaacat aacgtacaca
    48181 ataatattcc ggcagtatgt gtcgctgcga tatgtgtcaa ttcaatatgt aacggtacac
    48241 ctgcaagttg ggctgcgtaa ataatgaaac cgaccaggca ggtaaaggcc catattgtcg
    48301 tcagacagta ttcaaaaaac agtggccaca ttcgaaagtt ttctttgcgc cacaaccttg
    48361 tcatattttt gaggaatact tctgcaccgc cctgagccca gcgcaggcgc tgattccaca
    48421 gcccttttaa cgtttcaggc attaatatcc agcacagcgc ccgtggctcg taaaaaatcg
    48481 tccactgatt caactgcagc ttccagctaa tatcaatatc ttcggtgatc atatcgtcac
    48541 tccagtaacc cacttctgcc agggcgctgc gacgaaatgc ggcaataaca ccggaaacgg
    48601 taaatacgtt tccatagata cgctgggtcc gcttgatcaa accaataatt gaggaatact
    48661 cgccaacctg aattttaccc accagggtag aacgtgtgcg aatacgagga ttaccggtta
    48721 cggcacccac acgcgggttg tacaacatcg gttccacaat atatgccgct gcatcgcggt
    48781 ctaataacgc gtcgccatca atgcacacca gatattcact tttcgccgcg gcagctccgg
    48841 ttttaagcgc aatggctttc ccctggtttt gcgccagatg aatgacccgc agacggggaa
    48901 tttgtgcagc catgcgatcc aggatggcac gggttttgtc tgttgaacca tcatttacgg
    48961 caataacttc aatgttctca taacgctgtg ctaaagcggc atgtatggtt tcttcaacgt
    49021 ttttctcctc attaaaacag ggaatgataa tggagataga tggattatct ttcaactggg
    49081 gggctggtgc gttttctccc cacggccagt ggcgttcacg atagacccag aaatagacgc
    49141 cgccaataat ccacataatg gacataaaaa acggccagaa gaaaacgaac ctcatcatca
    49201 gttcaccaga gtgaaagtac gctacgcata ggggtatgca taacaccaga cataatataa
    49261 aaaacgatac gatgcgatta atcatttttc ggataccagg ctgttgaaaa ctcaggacga
    49321 ataacgtcta tttcaggttg gttatgcaga aaattgtcgg gataataacc ataatttttc
    49381 actccattca gttgtaatag gctcatccag tgagcgagtt gttgtgaaga aata
//
LOCUS       DAHHTG010000018        48408 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.018, whole genome shotgun sequence.
ACCESSION   DAHHTG010000018 DAHHTG010000000
VERSION     DAHHTG010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 48408)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48408)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48408
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.018"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            122..364
                     /locus_tag="NBI74_001050"
     CDS             122..364
                     /locus_tag="NBI74_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1931417.1"
                     /translation="MKKVLYGIFAISALAATSAWAAPVQVGEAAGSAATSVSAGSSSA
                     TSVSTVSSAVGVALAATGGGDGSNTGTTTTTTTSTQ"
     gene            479..1117
                     /locus_tag="NBI74_001051"
     CDS             479..1117
                     /locus_tag="NBI74_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbF family lipoprotein"
                     /protein_id="HCD1931418.1"
                     /translation="MKRPALILICLLLQACSATTKELGNSLWDSLFGTPGVQLTDDDI
                     QNMPYASQYMQLNGGPQLFVVLAFAEDGQQKWVTQDQATLVTQHGRLVKTLLGGDNLI
                     EVNNLAADPLIKPAQIVDGATWTRTMGWTEYQQVRYATARSVFKWDGTDTVKVGSDET
                     PVRVLDEEVSTDQARWHNRYWIDSEGQIRQSEQYLGADYFPVKTTLIKAAKQ"
     gene            1114..1851
                     /locus_tag="NBI74_001052"
     CDS             1114..1851
                     /locus_tag="NBI74_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC family protein"
                     /protein_id="HCD1931419.1"
                     /translation="MIKQTIVALILSVGASSVFAAGTVKVFSNGSSDAKTLTGAEHLI
                     DLVGQPRLANSWWPGAVISEELATAAALRQQQALMTRMAELAADSSADDAAAINALRQ
                     QIQALKVTGRQKINLDPDIVRVAERGNPPLQGNYTLWVGPPPSTVTLFGLISRPGKQS
                     FTPGRDVASYLSDQSLLSGADRSYAWVVYPDGRTQKAPVAYWNKRHVEPMPGSIIYVG
                     LADSVWSETPDALNADILQTLTQRIPQ"
     gene            1851..3947
                     /locus_tag="NBI74_001053"
     CDS             1851..3947
                     /locus_tag="NBI74_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HCD1931420.1"
                     /translation="MKKRHLLSLLALGISTACYGETYPAPIGPSQSDFGGVGLLQTPT
                     ARMAREGELSLNYRDNDQYRYYSASVQLFPWLETTLRYTDVRTRQYSSVEAFSGDQTY
                     KDKAFDLKLRLWEESYWLPQVAVGARDIGGTGLFDAEYLVASKAWGPFDFTLGLGWGY
                     LGTSGNVKNPLCSASDKYCYRDNSYKQAGSIDGSQMFHGPASLFGGVEYQTPWQPLRL
                     KLEYEGNNYQQDFAGKLEQKSKFNVGAIYRVTDWADVNLSYERGNTFMFGVTLRTNFN
                     DLRPSYNDNARPQYQPQPQDAILQHSVVANQLTLLKYNAGLADPQIQAKGDTLYVTGE
                     QVKYRDPREGIIRANRIVMNDLPDGIKTIRITENRLNMPQVTTETDVASLKNHLAGEP
                     LGHETKLAQKRVEPVVPKSTEQGWYIDKSRFDFHIDPVLNQSIGGPENFYMYQLGVMG
                     TADLWLTDHLLTTGSLFANLANNYDKFNYTNPPQDSHLPRVRTHVREYVQNDVYVNNL
                     QANYFQHLGNGFYGQVYGGYLETMFGGVGAEVLYRPLDSNWAFGLDANYVKQRDWRSA
                     KDMMKFTDYSVKTGHLTAYWTPSFAQDVLVKASVGQYLAGDKGGTLEIAKRFDSGVVV
                     GGYATITNVSKEEYGEGDFTKGVYVSVPLDLFSSGPTRSRAAIGWTPLTRDGGQQLGR
                     KFQLYDMTSDRSVNFR"
     gene            complement(3994..4272)
                     /locus_tag="NBI74_001054"
     CDS             complement(3994..4272)
                     /locus_tag="NBI74_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1931421.1"
                     /translation="MKRNLIKVVKMKPYFAALMLSVSVLPAYAGPLGTADKADLPQSN
                     VSSPMMAQSLRQPDLQPISTDRKTECFRLYTPDRKPGVNCVPDGSTGH"
     gene            4487..4897
                     /gene="psiE"
                     /locus_tag="NBI74_001055"
     CDS             4487..4897
                     /gene="psiE"
                     /locus_tag="NBI74_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-starvation-inducible protein PsiE"
                     /protein_id="HCD1931422.1"
                     /translation="MTSLSRPRVEFISTILQTVLNLGLLCLGLILVVFLGKETVHLAD
                     VLFAPEQTSKYELVEGLVVYFLYFEFIALIVKYFQSGFHFPLRYFVYIGITAIVRLII
                     VDHKSPLDVLIYSAAILLLVITLWLCNSKRLKRE"
     gene            complement(4991..5881)
                     /gene="malG"
                     /locus_tag="NBI74_001056"
     CDS             complement(4991..5881)
                     /gene="malG"
                     /locus_tag="NBI74_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001252048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalG"
                     /protein_id="HCD1931423.1"
                     /translation="MAMVQPKSQKARLFITHLLLLLFIAAIMFPLLMVVAISLRQGNF
                     ATGSLIPEQISWDHWKLALGFSVEQADGRITPPPFPVLLWLWNSVKVAGISAIGIVAL
                     STTCAYAFARMRFPGKATLLKGMLIFQMFPAVLSLVALYALFDRLGEYIPFIGLNTHG
                     GVIFAYLGGIALHVWTIKGYFETIDSSLEEAAALDGATPWQAFRLVLLPLSVPILAVV
                     FILSFIAAITEVPVASLLLRDVNSYTLAVGMQQYLNPQNYLWGDFAAAAVMSALPITI
                     VFLLAQRWLVNGLTAGGVKG"
     gene            complement(5896..7440)
                     /gene="malF"
                     /locus_tag="NBI74_001057"
     CDS             complement(5896..7440)
                     /gene="malF"
                     /locus_tag="NBI74_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalF"
                     /protein_id="HCD1931424.1"
                     /translation="MDVIKKKHWWQSDALKWSVLGLLGLLVGYLVVLMYAQGEYLFAI
                     TTLILSSAGLYIFANRKAYAWRYVYPGMAGMGLFVLFPLVCTIAIAFTNYSSTNQLTF
                     ERAQEVLLDRSWQAGKIYNFGLYPAGDEWQLALSDGETGKNYLSDAFKFGGKQKLQLK
                     ETTAQPEGERANLRVITQNRQALSDITAILPDGNKVMMSSLRQFSGTQPLYTLDGDGT
                     LTNNQSGVKYRPNDQIGFYQSITADGNWGDEKLSPGYTVTTGWKNFTRVFTDEGIQKP
                     FLAIFVWTVVFSLITVFLTVAVGMVLACLVQWEALRGKAVYRVLLILPYAVPSFISIL
                     IFKGLFNQSFGEINMMLSALFGVKPAWFSDPTTARTMLIIVNTWLGYPYMMILCMGLL
                     KAIPDDLYEASAMDGAGPFQNFFKITLPLLIKPLTPLMIASFAFNFNNFVLIQLLTNG
                     GPDRLGTTTPAGYTDLLVNYTYRIAFEGGGGQDFGLAAAIATLIFLLVGALAIVNLKA
                     TRMKFD"
     gene            complement(7594..8784)
                     /gene="malE"
                     /locus_tag="NBI74_001058"
     CDS             complement(7594..8784)
                     /gene="malE"
                     /locus_tag="NBI74_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000695396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter
                     substrate-binding protein MalE"
                     /protein_id="HCD1931425.1"
                     /translation="MKIKTGARILALSALTTMMFSASALAKIEEGKLVIWINGDKGYN
                     GLAEVGKKFETDTGIKVTVEHPDKLEEKFPQVAATGDGPDIIFWAHDRFGGYAQSGLL
                     AEITPDKAFQDKLYPFTWDAVRYNGKLIAYPIAVEALSLIYNKDLLPNPPKTWEEIPA
                     LDKELKAKGKSALMFNLQEPYFTWPLIAADGGYAFKYENGKYDIKDVGVDNAGAKAGL
                     TFLVDLIKNKHMNADTDYSIAEAAFNKGETAMTINGPWAWSNIDTSKVNYGVTVLPTF
                     KGQPSKPFVGVLSAGINAASPNKELAKEFLENYLLTDEGLEAVNKDKPLGAVALKSYQ
                     DELAKDPRIAATMDNAQKGEIMPNIPQMSAFWYAVRTAVINAASGRQTVDSALKDAQT
                     RITK"
     gene            9149..10264
                     /gene="malK"
                     /locus_tag="NBI74_001059"
     CDS             9149..10264
                     /gene="malK"
                     /locus_tag="NBI74_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011915502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter ATP-binding
                     protein MalK"
                     /protein_id="HCD1931426.1"
                     /translation="MASVQLQNVTKAWGEVVVSKDINLDIHEGEFVVFVGPSGCGKST
                     LLRMIAGLETITSGDLFIGEKRMNDTPPAERGVGMVFQSYALYPHLSVAENMSFGLKL
                     AGAKKEVINQRVNQVAEVLQLAHLLDRKPKALSGGQRQRVAIGRTLVAEPSVFLLDEP
                     LSNLDAALRVQMRIEISRLHKRLGRTMIYVTHDQVEAMTLADKIVVLDAGRVAQVGKP
                     LELYHYPADRFVAGFIGSPKMNFLPVKVTATAIDQVQVELPMPNRQQVWLPVESRDVQ
                     VGANMSLGIRPEHLLPSDIADVILEGEVQVVEQLGNETQIHIQIPSIRQNLVYRQNDV
                     VLVEEGATFAIGLPPERCHLFREDGTACRRLHKEPGV"
     gene            10336..11676
                     /gene="lamB"
                     /locus_tag="NBI74_001060"
     CDS             10336..11676
                     /gene="lamB"
                     /locus_tag="NBI74_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltoporin LamB"
                     /protein_id="HCD1931427.1"
                     /translation="MMITLRKLPLAVAVAAGVMSAQAMAVDFHGYARSGIGWTGSGGE
                     QQCFQTTGAQSKFRLGNECETYAELKLGQEVWKEGDKSFYFDTNVAYSVAQQNDWEAT
                     DPAFREANVQGKNLIEWLPGSTIWAGKRFYQRHDVHMIDFYYWDISGPGAGLENIDVG
                     FGKLSLAATRSSEAGGSSSFASNNIYDYTNETANDVFDVRLAQMEINPGGTLELGVDY
                     GRANLRDNYRLVDGASKDGWLFTAEHTQSVLKGFNKFVVQYATDSMTSQGKGLSQGSG
                     VAFDNEKFAYNINNNGHMLRILDHGAISMGDNWDMMYVGMYQDINWDNDNGTKWWTVG
                     IRPMYKWTPIMSTVMEIGYDNVESQRTGDKNNQYKITLAQQWQAGDSIWSRPAIRVFA
                     TYAKWDEKWGYDYTGSSSTNPYYGKAVSADFNGGSFGRGDSDEWTFGAQMEIWW"
     gene            11908..12828
                     /gene="malM"
                     /locus_tag="NBI74_001061"
     CDS             11908..12828
                     /gene="malM"
                     /locus_tag="NBI74_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose operon protein MalM"
                     /protein_id="HCD1931428.1"
                     /translation="MKMNKSLIALCLSAGLLASAPGISLADVNYVPQNTSDAPAIPSA
                     ALQQLTWTPVDQSKTQTTQLSTGGQQLNVSGISGPVAAYSVPANIGELTLTLTSEVNK
                     QTSVFAPNVLILDQNMTPSAFFPSSYFTYQEPGVMSADRLEGVMRLTPALGQQKLYVL
                     VFTTEKDLQQTTQLLDPAKAYAKGVGNSVPDIPDPIARHITDGLLKLKVKTNSSSSVL
                     VGPLFGSSAPAPVTVGNTAAPAVAAPAPAPVKKSEPMLNDTESYFNTAIKNAVAKGDV
                     DKALKLLDEAERLGSTSARSTFISSVKGKG"
     gene            13007..13504
                     /gene="ubiC"
                     /locus_tag="NBI74_001062"
     CDS             13007..13504
                     /gene="ubiC"
                     /locus_tag="NBI74_001062"
                     /EC_number="4.1.3.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate lyase"
                     /protein_id="HCD1931429.1"
                     /translation="MSHPALTQLRALRYFTEIPALEPQLLDWLLLEDSMTKRFEQQGK
                     TVSVTMIREGFVEQNEIPEELPLLPKESRYWLREILLCADGEPWLAGRTVVPVSTLSG
                     PELALQKLGKTPLGRYLFTSSTLTRDFIEIGRDAGLWGRRSRLRLSGKPLLLTELFLP
                     ASPLY"
     gene            13517..14389
                     /gene="ubiA"
                     /locus_tag="NBI74_001063"
     CDS             13517..14389
                     /gene="ubiA"
                     /locus_tag="NBI74_001063"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HCD1931430.1"
                     /translation="MEWSLTQNKLLAFHRLMRTDKPIGALLLLWPTLWALWVATPGVP
                     QLWILAVFVAGVWLMRAAGCVVNDYADRKFDGHVKRTANRPLPSGAVTEKEARTLFVV
                     LVLISFLLVLTLNTMTILLSIAALALAWVYPFMKRYTHLPQVVLGAAFGWSIPMAFAA
                     VSESVPLSCWLMFLANILWAVAYDTQYAMVDRDDDVKIGIKSTAILFGQYDKLIIGIL
                     QIGVLALMAIIGELNGLGWGYYWSILVAGALFVYQQKLIANREREACFKAFMNNNYVG
                     LVLFLGLAMSYWHF"
     gene            complement(14544..16967)
                     /gene="plsB"
                     /locus_tag="NBI74_001064"
     CDS             complement(14544..16967)
                     /gene="plsB"
                     /locus_tag="NBI74_001064"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsB"
                     /protein_id="HCD1931431.1"
                     /translation="MSGWPRIYYKLLNLPLSILVKSKSIPADPAPELGLDTSRPIMYV
                     LPYNSKADLLTLRAQCLAHDLPDPLEPLEIDGTLLPRYVFIHGGPRVFTYYTPKEESI
                     KLFHDYLDLHRSNPNLDVQMVPVSVMFGRAPGREKGEVNPPLRMLNGVQKFFAVLWLG
                     RDSFVRFSPSVSLRRMADEHGTDKTIAQKLARVARMHFARQRLAAVGPRLPARQDLFN
                     KLLASRAIAKAVEDEARSKKISHEKAQQNAIALMEEIAANFSYEMIRLTDRILGFTWN
                     RLYQGINVHNAERVRQLAHDGHELVYVPCHRSHMDYLLLSYVLYHQGLVPPHIAAGIN
                     LNFWPAGPIFRRLGAFFIRRTFKGNKLYSTVFREYLGELFSRGYSVEYFVEGGRSRTG
                     RLLDPKTGTLSMTIQAMLRGGTRPITLIPIYIGYEHVMEVGTYAKELRGATKEKESLP
                     QMLRGLSKLRNLGQGYVNFGEPMPLMTYLNQHVPDWRESIDPIEAVRPAWLTPTVNNI
                     AADLMVRINNAGAANAMNLCCTALLASRQRSLTREQLTEQLNCYLDLMRNVPYSTDST
                     VPSASASELIDHALQMNKFEVEKDTIGDIIILPREQAVLMTYYRNNIAHMLVLPSLMA
                     AIVTQHRHISRDVLMEHVNVLYPMLKAELFLRWDRDELPDVIDALANEMQRQGLITLQ
                     DDELHINPAHSRTLQLLAAGARETLQRYAITFWLLSANPSINRGTLEKESRTVAQRLS
                     VLHGINAPEFFDKAVFSSLVLTLRDEGYISDSGDAEPAETMKVYQLLAELITSDVRLT
                     IESATQGEG"
     gene            17138..17506
                     /gene="dgkA"
                     /locus_tag="NBI74_001065"
     CDS             17138..17506
                     /gene="dgkA"
                     /locus_tag="NBI74_001065"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HCD1931432.1"
                     /translation="MANNTTGFIRIIKAAGYSWKGLRAAWINEAAFRQEGVAVLLAVV
                     IACWLDVDAITRVLLISSVMLVMIVEILNSAIEAVVDRIGSEYHELSGRAKDMGSAAV
                     LIAIIVAVITWCILLWSHFG"
     gene            17616..18224
                     /gene="lexA"
                     /locus_tag="NBI74_001066"
     CDS             17616..18224
                     /gene="lexA"
                     /locus_tag="NBI74_001066"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000646075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repressor LexA"
                     /protein_id="HCD1931433.1"
                     /translation="MKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAE
                     EHLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAAGEPLLAQQHIEGHYQVDP
                     SLFKPNADFLLRVSGMSMKDIGIMDGDLLAVHKTQDVRNGQVVVARIDDEVTVKRLKK
                     QGNKVELLPENSEFKPIVVDLRQQSFTIEGLAVGVIRNGDWL"
     gene            18297..19622
                     /gene="dinF"
                     /locus_tag="NBI74_001067"
     CDS             18297..19622
                     /gene="dinF"
                     /locus_tag="NBI74_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter DinF"
                     /protein_id="HCD1931434.1"
                     /translation="MAFLTSSDKALWHLALPMIFSNITVPLLGLVDTAVIGHLDSPVY
                     LGGVAVGATATSFLFMLLLFLRMSTTGLTAQAYGAKNPQALARALVQPLLLALGAGAL
                     IALLRTPIIDLALHIVGGSEAVLEQARRFLEIRWLSAPASLANLVLLGWLLGVQYARA
                     PVILLVVGNILNIVLDVWLVMGLHMNVQGAALATVIAEYATLLIGLLMVRKILKLRGI
                     SGEMLKTAWRGNFRHLLALNRDIMLRSLLLQLCFGAITVLGARLGSDIIAVNAVLMTL
                     LTFTAYALDGFAYAVEAHSGQAYGARDGSQLLDVWRAACRQSGIVALLFSVVYLLAGE
                     HIIALLTSLTQIQQLADRYLIWQVILPLVGVWCYLLDGMFIGATRAAEMRNSMAVAAA
                     GFALTLLTLPWLGNHGLWLALTVFLALRGLSLAAIWRRHWRNDTWFVAT"
     gene            19738..19947
                     /locus_tag="NBI74_001068"
     CDS             19738..19947
                     /locus_tag="NBI74_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HCD1931435.1"
                     /translation="MNKDEAGGNWKQFKGKVKEQWGKLTDDDMTIIEGKRDQLVGKIQ
                     ERYGYQKDQAEKEVDSWEKRHDYRW"
     gene            complement(19989..20504)
                     /gene="zur"
                     /locus_tag="NBI74_001069"
     CDS             complement(19989..20504)
                     /gene="zur"
                     /locus_tag="NBI74_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc uptake transcriptional repressor Zur"
                     /protein_id="HCD1931436.1"
                     /translation="MEKTTTQELLAQAEKICAQRNVRLTPQRLEVLRLMSLQDGAISA
                     YDLLDLLREAEPQAKPPTVYRALEFLLEQGFVHKVESTNSYVLCHLFDQPTHTSAMFI
                     CDRCGAVKEECAEGVEDIMHTLAAKMGFALRHNVIEAHGLCSACVEVEACRHPEQCHH
                     DHSIQVKKKPR"
     gene            20660..21655
                     /gene="dusA"
                     /locus_tag="NBI74_001070"
     CDS             20660..21655
                     /gene="dusA"
                     /locus_tag="NBI74_001070"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HCD1931437.1"
                     /translation="MPAESHTVYPAYRFSIAPMLDWTDRHCRYFLRLLSRNTLLYTEM
                     VTTGAIIHGKGDYLAYSEEEHPVALQLGGSDPAALAQCAKLAEARGYDEINLNVGCPS
                     DRVQNGMFGACLMGNAQLVADCVKAMRDVVSIPVTVKTRIGIDDQDSYEFLCDFINTV
                     SGKGECEMFIIHARKAWLSGLSPKENREIPPLDYPRVYQLKRDFPHLTMSINGGIKSL
                     EEAKAHLQHMDGVMVGREAYQNPGILAAVDREIFGSSDTDADPVAVVRAMYPYIEREL
                     SQGTYLGHITRHMLGLFQGIPGARQWRRYLSENAHKAGADINVLEHALKLVADKR"
     gene            21789..22031
                     /gene="pspG"
                     /locus_tag="NBI74_001071"
     CDS             21789..22031
                     /gene="pspG"
                     /locus_tag="NBI74_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response protein PspG"
                     /protein_id="HCD1931438.1"
                     /translation="MLELLFVIGFFVMLMVTGVSLLGIIAALIVATAFMFLGGMLALM
                     IKLLPWLLLAVAVVWVIRAIKAPKVPKYQHYDRWRY"
     gene            complement(22196..23179)
                     /locus_tag="NBI74_001072"
     CDS             complement(22196..23179)
                     /locus_tag="NBI74_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HCD1931439.1"
                     /translation="MATRIEFHKHGGPEVLQAVEFTPADPAENEIQVKNKAIGINFID
                     TYIRSGLYPPPSLPSGLGTEAAGIVSKVGSGVKHIKAGDRVVYAQSALGAYSSMHNIN
                     ADKAAILPAAISFEQAAASFLKGLTVYYLLRKTYEIKPDEQFLFHAAAGGVGLIACQW
                     AKALGAKLIGTVGTAQKAQSALKAGAWQVINYREENLVERLKEITGGKKVRVVYDSVG
                     RDTWERSLDCLQRRGLMVSFGNSSGAVTGVNLGILNQKGSLYVTRPSLQGYITTREEL
                     TEASNELFSLIASGVIKVDVAEQQKYPLKDARRAHEILESRATQGSSLLIP"
     gene            complement(23285..25321)
                     /locus_tag="NBI74_001073"
     CDS             complement(23285..25321)
                     /locus_tag="NBI74_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000564684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HCD1931440.1"
                     /translation="MIDETLTALTDIKMPHYAPLAQASGSIDLNGTILPEYCCNTLVL
                     GSGAAGWRAAVELKRQNVDVMVASSKAFWGTSACSGSDKQTLHTANTRANGDHFLTLS
                     NTLAAGGAMDHDTAYVEAVGSVNTCEVLKYLGLDLPEDLFGATLRYQTDHDEFGRATS
                     CGPRTSRLMVKVLAQEAMRLNIPLCDHTTAIHILTSGEGENRRVVGVIAIDKACRDNP
                     WRMVVIRCQHLVLATGGPGELYRDSVYPVNCFSALGMALEAGITLVNLTESQFGIGTP
                     RSQFPWNLSGTYMQAMPRIYSQDHSGRQYNFLAAYYPTTRMLASAIFRKGYQWPFHAE
                     RMLEYGSSLLDVAVYEETQKGRDVFLDFLREPEPAADGTSFNLQELDDDVIDYLQQNH
                     ALLAQPLARLTQMNPLAIRLYQMHGHDLTASPLKFTLNNQHLNGGIEVDIWGRTSLTG
                     CYAAGENAGTHGVTRPGGAALNAGQVFARRCALHIAHQKSQDRPLERQQILSTINEAQ
                     QYLNQGLLRDDVRETIQNTMSHHAAILCHTQGINTAAQTLATLCEDIRREGIQCDENS
                     LAQSFQWRQSAQLALAVLRSLQCYVENGGGSRGARAIYDGEAGIAPQTPGGPLLAWRF
                     RPENQDARQYMICVCREANDYQTWTRPCRERGVLTLTNFERQWQNWIAATPFQY"
     gene            complement(25332..26612)
                     /locus_tag="NBI74_001074"
     CDS             complement(25332..26612)
                     /locus_tag="NBI74_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001057569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HCD1931441.1"
                     /translation="MNMTTSVPQTPSLFRRIMTKLAAFAPGIFLIGYSIGTGSITTMA
                     KAGANYGVSLMWAVALSCIVTWFLIRIYGRYASVTGERALTAFRRHIHPGVAMLFLIG
                     LTVAVSGSIMGVMGILADVMATWSTTWFSGGISALAWAIAMVIMINVMLWTNSNAGFD
                     KILSALVGIMGLAFVINFFIMMPPVADLLAGFIPSVPDPVAGDNSTPFLIISGMVGTT
                     VAPAMFLMRTTLVKSNGWGVDNLPHQNRDAIVSAGMMFIISAAVMAAAAGTLYQTGTG
                     LNNASQMVIILEPIAGSAAVGLFVIGIVAAGLSSQFPNLLLTPWLLADWRDTSNEVSP
                     GYRILMTVMSLLCLVVPLFHARPVLVMIASQAFNAIILPLTVLCMFWLANKPSLMGKY
                     RCSRWENVMFIAISVFTLIMGYMAMSGLIESLMK"
     gene            complement(26639..27418)
                     /locus_tag="NBI74_001075"
     CDS             complement(26639..27418)
                     /locus_tag="NBI74_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HCD1931442.1"
                     /translation="MNNGTIDLQRNGFIATLVINRPAKLNALTPEMLEQLLAHCHTLE
                     TDSEIRVVLLTSASEKAFCVGADIHRWTSLSALDMWRNWIRRGHQAFDALAQLPQPVI
                     ALLHGLAYGGGLELAATADIRICSAEAQMALPETGLATVPGWSGTQRLTALLGRSLVK
                     ELVFTGEPLDAQRALSCGLVNRVVAREALHDAGLQLAQRIAQRAPVAVQIAKQVIDAG
                     EGIAVASTLEALAGAFSATTEDAHEGSQAFTEKRQPHFQAR"
     gene            complement(27411..28982)
                     /locus_tag="NBI74_001076"
     CDS             complement(27411..28982)
                     /locus_tag="NBI74_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl CoA:acetate/3-ketoacid CoA transferase"
                     /protein_id="HCD1931443.1"
                     /translation="MCSVRTITAPEAADLIVDGAVVTVSSSSGMGCPDAVLAAIGERF
                     QRAGHPQNITSIHPIAAGDMYGVKGIDHLAQPGLLAKVIAGSYPSGPSSMTPPLIWQM
                     INDNAIPAWNLPSGILFDMHREAAAHRPGVLTQTGMDTYVDPLLQGGAMNEKAAQQSL
                     VERVRFANDDWLFFPSIVPQVAIIRATTADERGNLTYEHEGAYLGPLEQATAVRNNGG
                     IIIAQVKRQVAAGSLKPKEVRIPGVLVDYIVIAPEQTQTTQTQYEPAISGEISRPLSA
                     FRYMEHGPARVIAQRVAQELQSGDAVNIGFGISANVPRILLEQGRHGDVTWLLEQGAI
                     GGVPLLEFQFGCASNAEAFLPSPQQFTYFQGGGFDLTLMSFLQIGADGSVNVSHLPAR
                     PHVTAGCGGFIDITSHAKRIIFSGFFNAGAQLQLEEGQLRIIKEGKAKKLVQDVAHVT
                     FSGKRAIRLGQQVQYITERCVLELTPDGLLVTEIAPGIDPERDILAQSDAPLRLADDL
                     KLMNPLYFQQGSHHE"
     gene            29238..30272
                     /locus_tag="NBI74_001077"
     CDS             29238..30272
                     /locus_tag="NBI74_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001129782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCD1931444.1"
                     /translation="MAKSDTGRKRVTLTDVARAAGVSKSTVSLVLNDSSLIKKETQQK
                     VQQAIEQLGYVYNRFAANLRSQKSLTIGVVIDDLINPFFAEFTMGLEMTLAEHGFITV
                     MSNTSQRSDRQKQVLDTLLEHHVAGIVLCPVNSTSEADLQRYANSSTPLLITMRPLDW
                     QQLPVDYVGVDSHAGVREATEYLIQQGHRDIVFIGGLTHHMRYQGYLEAMNHHGLQPW
                     STDAFSLRAEPTQANGYQLMQQLLDMPSPPTAVICYNDLMAFGAESALGERGLFAGED
                     ISLIGNDGVAACAYSNPPLTTIAVEPLALGKQAAQQILRRIAQPDAPLSHYIYRPTLQ
                     IRASTGKRGR"
     gene            30345..31760
                     /gene="dnaB"
                     /locus_tag="NBI74_001078"
     CDS             30345..31760
                     /gene="dnaB"
                     /locus_tag="NBI74_001078"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HCD1931445.1"
                     /translation="MAGNRPFNKQQTDNRERDPQVAGLKVPPHSIEAEQSVLGGLMLD
                     NERWDDVAERVVADDFYTRPHRHIFTEMARLQESGSPIDLITLAESLERQGQLDSVGG
                     FAYLAELSKNTPSAANISAYADIVRERAVVREMISVANEIAEAGFDPQGRTSEDLLDL
                     AESRVFKIAESRANKDEGPKNIADVLDATVARIEQLFQQPHDGVTGVNTGYDDLNKKT
                     AGLQPSDLIIVAARPSMGKTTFAMNLVENAAMLQDKPVLIFSLEMPSEQIMMRSLASL
                     SRVDQTKIRTGQLDDEDWARISGTMGILLEKRNIYIDDSSGLTPTEVRSRARRIAREH
                     GGIGLIMIDYLQLMRVPALSDNRTLEIAEISRSLKALAKELNVPVVALSQLNRSLEQR
                     ADKRPVNSDLRESGSIEQDADLIMFIYRDEVYHENSDLKGIAEIIIGKQRNGPIGTVR
                     LTFNGQWSRFDNYAGPQYDDE"
     gene            31813..32892
                     /gene="alr"
                     /locus_tag="NBI74_001079"
     CDS             31813..32892
                     /gene="alr"
                     /locus_tag="NBI74_001079"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HCD1931446.1"
                     /translation="MQAATVVINRRALRHNLQRLRELAPASKMVAVVKANAYGHGLLE
                     TARTLPDADAFGVARLEEALRLRAGGITKPVLLLEGFFDARDLPTISAQHFHTAVHNE
                     EQLAALEEANLDEPVTVWMKLDTGMHRLGVRPEQAEAFYHRLTQCKNVRQPVNIVSHF
                     ARADEPKCGATEKQLAIFNTFCEGKPGQRSIAASGGILLWPQSHFDWVRPGIILYGVS
                     PLEDRSTGADFGCQPVMSLTSSLIAVREHKAGEPVGYGGTWVSERDTRLGVVAMGYGD
                     GYPRAAPSGTPVLVNGREVPIVGRVAMDMICVDLGPQAQDKAGAPVILWGEGLPVERI
                     AEMTKVSAYELITRLTSRVAMKYVD"
     gene            32915..33472
                     /locus_tag="NBI74_001080"
     CDS             32915..33472
                     /locus_tag="NBI74_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000122236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide transporter"
                     /protein_id="HCD1931447.1"
                     /translation="MSYSEIAACLAYAVSVWLAARNNVHTWWIGIIGSILYGWVFWSV
                     QLYADVTLQLFFIVTSITGWIHWLKGQGGDILPVRRTQASHFFLLLLCAVVVAGGYGF
                     LLHTFTNAWAPWLDSLILTFSVLAQFMLMGRRIENWYVWLAVNTLAVPLYMTRGLNLT
                     AGLYFLFWINAWYGLYQWRKELQTS"
     gene            33469..34473
                     /locus_tag="NBI74_001081"
     CDS             33469..34473
                     /locus_tag="NBI74_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001601110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCD1931448.1"
                     /translation="MKPFATGLVVGKFAPLHCGHEKLINTALAQCEELFIISYSVPEM
                     PDCEPEKRLTWLQVRFPQATFLVLTPELVARYNLPAIPHNNADADIHRHYVATLCLQI
                     LRCRPHAVFTAEDYGDGFANVLARRFAQPVEHVRMARPVGDEAPSGTLIRSDVHRYRY
                     MLANDVYYSFVRRICLLGGESTGKSTLSKALAGVLDTVYVAEFGRDYWEEKNGILTAD
                     DLLHIACEQVRREQQAEANHYLICDTSPLTTLFYALDQYGHAPQELHQLAEREYSLVV
                     LCGAEFPFVQDGTRQGEVFRARQQVWYEQELSRRNIPYLSVSGPLQERVEQILRQLPD
                     "
     gene            34555..35748
                     /gene="tyrB"
                     /locus_tag="NBI74_001082"
     CDS             34555..35748
                     /gene="tyrB"
                     /locus_tag="NBI74_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transaminase"
                     /protein_id="HCD1931449.1"
                     /translation="MFQKVDAYAGDPILTLMERFKEDPRSDKVNLSIGLYYNEDGIIP
                     QLKAVADAEARLNAQPHGASLYLPMEGLNSYRHAIAPLLFGADHPVLQQQRVATIQTL
                     GGSGALKVGADFLKRYFPESSVWVSDPTWENHVAIFAGAGFEVSTYPWYDEATNGVRF
                     NDLLATLKTLPARSIVLLHPCCHNPTGADLTNEQWDAVIEILKARELIPFLDIAYQGF
                     GAGMEEDAYAIRAIASAGLPALVSNSFSKIFSLYGERVGGLSVLCEDAEAAGRVLGQL
                     KATVRRNYSSPPNFGAQVVAAVLNDEALKASWLVEVEEMRTRILAMRQELVKVLSTEM
                     PERNFDYLLNQRGMFSYTGLSAAQVDQLREEFGVYLIASGRMCVAGLNTQNVHRVAKA
                     FAAVM"
     gene            35930..38674
                     /locus_tag="NBI74_001083"
     CDS             35930..38674
                     /locus_tag="NBI74_001083"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001468317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HCD1931450.1"
                     /translation="MENMTSPGTLLSGDNATWLEEYYQTWLRTPEQLPEDWRRFFLSP
                     ELTVQSVSGDNNISGATLKKQAAVIQLINAWRTQGHLRAKLDPLGLNPPADVPSLQPG
                     FWGLSEEDLLQEFSVTFGAHTTQMPLKQLLNLLEQAWASSQAYELAHLENREEINWLL
                     SRIESSNAPQADAQTCIARFEKLMAAETLERYLHTRYVGQKRFSLEGGESAIPALDTL
                     TKRLRAQGVEEMVIGMAHRGRLNVLVNLLNKDPAQLFAEFEGKQTIGSGSGDVKYHMG
                     YSSNLETPAGSLHVALAYNPSHLEIVNPVVLGQVRARQERRGEDGQAKVVGVLIHGDS
                     ALGGLGVNQTTFNLSQTQGYGTGGTLHLVINNQIGFTTSRLQDMRSSRYCTDIAKMVA
                     APIIHVNGDDVDAVCQVMELACEWRDTFRRDIIIDICCFRKHGHNESDEPRLTQPQMY
                     QAVDAHPGTLARYGESLARRGLLTQAQQDEMTARYRDWLDSCQKREPQPLKPAIHSFS
                     ANWYGLTNPHWSAPVSTALPRQKLAAYGEIISTLPPDVVAHPTIKRQLALRQDMAAGT
                     QPVDWGMAEMLAYASLVDAGVGVRLSGEDSGRGTFSHRHAVVHHQTEARRYLPLQHIR
                     AGQASFDVYDSVLNEEALLAFEYGYSTSAPQQLVIWEAQFGDFANGAQVAIDQFISSG
                     ETKWDRYSGLTILLPHGYDGQGPEHSSARPERWLQLCAENNMQVVMPSESAQMFHLLR
                     GQALRPMRKPLVIMMSKRLLRFKGAMSELCEFTDDAYKPVITDPQLHQSQKVKRVILC
                     SGQVYYDVLEARKQREHEDEVAIVRLEQLYPFPVAELNDVLASWPNCCEWIWLQEEPE
                     NQGAWRQIRHELAALKINTPYWQYAGRPAAAAPATGYGRVHKQQIDEFLAAAFADIQP
                     "
     gene            38707..39861
                     /gene="odhB"
                     /locus_tag="NBI74_001084"
     CDS             38707..39861
                     /gene="odhB"
                     /locus_tag="NBI74_001084"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001468318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HCD1931451.1"
                     /translation="MIEITVPVLPESVTEGTLTTWCKQEGEHVKRDDVIAELETDKVI
                     LEIPAPHDGVLSNIIVSEGSTVTSAQLLAHLKPQAVIEETVTPVTETLAMPSARLEAQ
                     RSGVELADVAGSGRNGRILKEDVQRVTPAPAIQPERVAEIAPAKPLTPGARQERREPM
                     SRLRQRIAERLLASQQNNAILTTFNEVNMQSVMDLRARWKDRFAEKHGVKLGFMSFFV
                     KAVTRALERFPVVNASVDGNEIIWRDYCDIGIAVSSNRGLVVPVLRNAQSLSLVEIER
                     QIAEYATQARNGKLPLEALQGGTFSITNGGTFGSMMSTPIINPPQSAILGMHAITPRP
                     VAENGQVVIRPMMYLALSYDHRIIDGQEAVHTLVAIRELLESPEQLLLDL"
     gene            39873..41291
                     /gene="lpdA"
                     /locus_tag="NBI74_001085"
     CDS             39873..41291
                     /gene="lpdA"
                     /locus_tag="NBI74_001085"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000103914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HCD1931452.1"
                     /translation="MSTIFDVAVMGGGPGGYVAALRAAQNGLSVVCIDDGVNAQGEPS
                     PGGTCLNVGCIPSKSLLQSSELYAQVQHEASIHGVNVDGVSFNAAAMIQRKDAIVSRL
                     TMGISLLFKKNKVKHLCGLATLECAQDEIWQLRVNDQHLHARNVVIATGSQPRQLPGV
                     TIDNQQILDNRGALALSEVPPRLGVIGAGVIGLELGSVWNRVGSDVTLLEMAPTFLPA
                     LEARLSNEVRKAMIASGMKMQLAVEIEAIEQRDDGVHVRWRQGEKREESRFDKLILAI
                     GRVPRLSGVDLVQLGLEADNRGGIAVDNLCRTGKAGLWAIGDVVRGPMLAHKAMAEGV
                     VVADQIAGLVVEPINFALIPSVIYTQPEVAWVGENEASLKAAGRVFNKGNSLFAGNGR
                     ALALGQEGGRCTLYSDKHTDRVLGGAIVGPQASELINEIALAMTFSASGEDIACAIHA
                     HPTLSEVIHEAAMALNNKALHG"
     gene            41313..42482
                     /gene="sucC"
                     /locus_tag="NBI74_001086"
     CDS             41313..42482
                     /gene="sucC"
                     /locus_tag="NBI74_001086"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001048620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HCD1931453.1"
                     /translation="MNLHEYQAKSLLAGMGMPCPKEIAIQQISQLADAWQHIACPSKG
                     AVLKAQVHAGGRGKAGGVKVLKQLPEAQAFVQQMLGSQLVTYQTGPEGQYVSSILLCE
                     NIYPVRQELYFGMVVDRESQRVTFIVSPEGGVEIEKVAHETPEKISSVSIDPLTGVQP
                     CHIREMFAVLQLEHGLFAAFSRLVNQAWKAFNELDFALLEINPLVLRETGEFMCADAK
                     VSLDDNALYRHPELQVLRDETQEDPRESQAAKLDLNYVSLDGNIGCMVNGAGLAMATM
                     DIIKLYGEQPANFLDVGGGATQERVSEAFRLIVSDSKVKAILVNIFGGIVRCDMIARA
                     IIHALNEASITLPVVVRLSGNNAAEGQRLLAESGLTVEAVDSLDDAAKRIIALLN"
     gene            42495..43367
                     /gene="sucD"
                     /locus_tag="NBI74_001087"
     CDS             42495..43367
                     /gene="sucD"
                     /locus_tag="NBI74_001087"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000114644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HCD1931454.1"
                     /translation="MSVLINKHTRVLVQGITGKNGTFHTEQAIAYGTQIVGGVTPGKG
                     GQRHLDRPVFDSMKDAMRQTEADASVIYVPAPFVLDSVVEAIEAGVKLVVVITEGVPT
                     LDMVKVRRLLDCHPDVRLIGPNCPGVITPGECKIGIMPGEIHRPGRIGIVSRSGTLTY
                     EAVAQTTALGLGQSTCIGIGGDPVPGTNFIDALRLFENDPQTDAVIMIGEIGGNAEER
                     AAEFIRHDMNKPVVGYIAGVTAPKGKRMGHAGAIISGGSGSAEDKFRAFDKAGMAWTR
                     NPALLGETLWNVIK"
     gene            43579..45078
                     /locus_tag="NBI74_001088"
     CDS             43579..45078
                     /locus_tag="NBI74_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DASS family sodium-coupled anion symporter"
                     /protein_id="HCD1931455.1"
                     /translation="MSSISHGAPQKRRIIPNPGLWLAIIAGIIITLLPLGDTLPVAGQ
                     NMIAILVFAIIVWISEAMDYTASAIVISALIIFMVGFAPDMNHPDTILGTAKALKMTL
                     SGFSNSALALVAAAMFIAAAMTITGLDKRIALFTMSKIGASSRSIIIGAIVVTIVLSL
                     VVPSATARTACVVPIMMGVIAAFKVDKHSRLAASMMIVIAQATSIWNVGIQTSAAQNL
                     LSIGFINKTFGAGHSVSWLDWLLAGAPWSLTMSVILYFLARKLLPPETEAVEGGSEAI
                     KKALAELGPTTGKEKRLIGISLLLLLFWSTGGKLHSIDTTSVTLAGLAIMLLPGIGVM
                     SWKEVEKRVQWGTLLMFGIGISLGSTLLDTQAASWMANYVVKGFGLDGLPSLAIFAIL
                     AAFLIIIHLGFASATALTAALLPILISLLSSLPPELGVNPVGMTILLAFSVSFGFILP
                     INAPQNMVCMGTDTFTPRQFTRVGLYLTVIGYLLLLLFAATWWKILGLM"
     gene            45090..46163
                     /locus_tag="NBI74_001089"
     CDS             45090..46163
                     /locus_tag="NBI74_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001304711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ldh family oxidoreductase"
                     /protein_id="HCD1931456.1"
                     /translation="MLLQIAVEKVRWLAAGLLELNGCDADIAQDVAEHMIEAERYGFA
                     SHGVTLLPKYLENIARGDVTANARPECLTSEGNLQRFHAHNGFGQHAGKVAVNAAIEQ
                     AKHRGFCLLTLCHAHHLGRMGHYGQMVADQGLAMLAMSNVTGRPALVAPWGGAEPRMT
                     TNPFCFAWPFSDGRPPILVDFATSSMALNKARVMSSTGKQAAPGQLIDAQGNPSNDPG
                     VLFSNPPGALLPFGEHKGFGLALMIEMMAGILSGGDTIAEDHQTDGSAHNHLFALIID
                     PDQFTDLAGEKGQFFADYLLATQPQPGGNPVIYPGMPEAANRERNAKMITIPVSFWSW
                     VVEHYARKGIDLGLHPQESALAG"
     gene            complement(46152..47510)
                     /locus_tag="NBI74_001090"
     CDS             complement(46152..47510)
                     /locus_tag="NBI74_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019841943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent Fis family transcriptional
                     regulator"
                     /protein_id="HCD1931457.1"
                     /translation="MNNKFSEITIVYIEDSDDVRFACEQTLTLAGYRVISCCDAEHSI
                     PLIQSQANIIILTDVRLPGISGLELLSYINEMDSKIPVILITGHGDVEMAVDAMRNGA
                     FDFIEKPSSSDKLLSIIARAVEKRRLVLENQQLLANLQQENGPVLIGRSPQMQQLRKM
                     ILNVADTGADVLIYGETGCGKEVVARMLHHWSTRRQGQFVALNCAGLPETLFESEIFG
                     HEAGAFTGAVKKRIGKIEHANGGTLFLDEIEGMPSGMQVKLLRVLQERTIERLGANQL
                     IPVNCRVIAATKEDLLRRSEEHLFRLDLYYRLNVVSLNIPPLRQRREDIPELFYWFAS
                     QAAQKYNRPLPDISPMLLAWLQSQSWPGNVRELKHNAERFVLGLLTHHQPVPMTQQEE
                     SGLTACIDAFEKKLIEDMLRQTEGQVSLTARLLQLPRKTLYDKLNKHQIQPQVYRPES
                     SS"
     gene            complement(47503..>48408)
                     /locus_tag="NBI74_001091"
     CDS             complement(47503..>48408)
                     /locus_tag="NBI74_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000704417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HCD1931458.1"
                     /translation="TLIIYLLFLLFIKYWRMRSHTQQLLTLANETLEKQVKERTSALE
                     LINQKLLQEMKERSQAEQVLQITRSELAESSKLAALGQMATEIAHEQNQPLAAIHALT
                     DNARIMLKKEMYPQVEQNLKHIISVIERMTQLISELKAFASRHRVPKGSADVIKVMYS
                     AVALLNHSMEKNNIERRIKAPSMPLFVNCDELGLEQIFSNLISNALDSMEGSSYKRLD
                     IAIRQANNKVIITIKDSGGGFAPEVVDRIFEPFFTTKRRGMGLGLAIVSEIVRNSNGA
                     LHASNHPEGGAVMTLTWPEWGEKHE"
BASE COUNT        11516 a        12088 c        12946 g        11858 t
ORIGIN      
        1 ttgtcctaaa actccatcac taattccccg cgccgtaatt cgcatgcttt agttgtgtat
       61 actcgatccc gcccgaaatg ttttgggtaa atctccattc attcaatgaa gggaaattgt
      121 tatgaaaaaa gttctgtatg gcatttttgc catatctgcg cttgcggcga cttctgcgtg
      181 ggctgcacct gtacaggtgg gcgaagcggc aggatcggca gcaacgtcgg tttcggcggg
      241 tagttcctcc gcgaccagcg tcagcaccgt aagctcggcg gtgggtgtcg cgctcgcggc
      301 aaccggtggc ggtgatggtt ctaataccgg gaccaccaca accacgacca ccagtaccca
      361 gtaataaagt atgtatcccc aaaataattc gagtcatcgc atctgaagct tgaagtatga
      421 aggggattaa ccataaccac actccggtgt ggttattctg cccctctgga gaagagtcgt
      481 gaagcgacca gcactcattc ttatctgcct gctattacag gcctgttcag ccacgactaa
      541 agagctgggc aattcactgt gggacagtct gttcggtacg ccaggcgtac agctgacgga
      601 cgatgatatt caaaatatgc cctacgccag ccagtacatg cagcttaatg gcgggccgca
      661 gttatttgtg gtgctggcct tcgctgaaga cggacaacaa aaatgggtca ctcaggatca
      721 ggccaccctt gtcacacaac atggtcgtct ggtgaagact ttgcttggcg gcgacaacct
      781 gattgaagtg aataaccttg ccgccgaccc gctgattaaa cccgcacaaa ttgttgatgg
      841 tgcaacctgg acgcgcacga tgggctggac cgagtaccag caggtacgct acgccaccgc
      901 acgctcagtc ttcaaatggg atggcaccga taccgtcaaa gtcggcagcg atgaaacccc
      961 ggttcgcgtg ctggacgaag aagtctccac cgaccaggcg cgctggcata accgctactg
     1021 gatcgacagc gaagggcaaa ttcgccagtc ggaacagtat ctcggcgcgg attacttccc
     1081 ggtgaaaacc accctcatca aggcggcaaa acaatgatta aacaaactat tgtcgcattg
     1141 attttgagtg tgggggcgtc atcggtcttc gcggcgggaa ccgtcaaggt attcagcaat
     1201 ggcagcagtg atgccaaaac gctgacgggt gcagagcatt taatcgatct ggtaggccaa
     1261 ccgcggctgg caaacagctg gtggcctggt gcggtgatta gcgaagagct ggccacggcg
     1321 gcagcattgc gtcagcagca ggcgttgatg acacggatgg cagaactggc cgcagactcc
     1381 agcgccgacg atgccgccgc aattaacgcc ttacgccagc aaattcaggc gttgaaagtg
     1441 acgggcaggc aaaaaatcaa tcttgatcct gatatcgtcc gcgttgccga acgcggtaac
     1501 ccgccgttgc agggcaacta cacgctgtgg gtcggaccgc cgccgtccac ggtcacgttg
     1561 ttcgggctta tcagtcgtcc tggcaagcag tcattcactc caggtcgcga cgtggcgagc
     1621 tatctctctg accagagcct gctcagcggt gcggatcgca gctacgcgtg ggtggtttac
     1681 ccggacggac gcacgcaaaa agcgccggtg gcttactgga acaagcgtca cgtagagccg
     1741 atgcccggca gcattattta tgttggcctc gcggactccg tctggagtga gacacctgat
     1801 gcccttaacg ccgacattct tcagactctg acgcagcgga taccccaata atgaaaaaaa
     1861 gacatctgct tagcttactg gcgctgggta ttagcacagc ttgctacggc gaaacatatc
     1921 ctgcgcccat tggtccgtcg caatcggatt tcggtggcgt aggattatta caaacgccca
     1981 ccgcgcgcat ggcgcgggaa ggggagctga gcctgaacta tcgcgataac gatcagtacc
     2041 gttattactc agcttcagtg caactcttcc cgtggctgga aacaacgctg cgctacaccg
     2101 acgtgcgcac ccggcagtac agcagcgtcg aagcgttctc tggcgatcaa acgtataaag
     2161 ataaagcctt cgatctcaaa ctgcgtttgt gggaagagag ttattggctg ccgcaagtgg
     2221 cggttggtgc gcgggatatc ggcggtacgg ggctgtttga tgcggaatat cttgttgcca
     2281 gcaaagcctg ggggccgttc gattttacac tcggcctggg ctggggatat ctgggcacca
     2341 gcggtaacgt gaaaaatccg ctctgttcag ccagtgataa atattgctat cgcgataaca
     2401 gctacaaaca ggcgggatct atcgacggca gccagatgtt ccacggcccg gcctcactgt
     2461 ttggcggcgt ggaataccag acgccctggc aaccgctgcg tctgaaactg gagtatgaag
     2521 gcaataatta tcagcaggat tttgccggaa agctggagca aaaaagtaag tttaacgtcg
     2581 gggcgattta tcgcgttacc gattgggccg acgttaacct tagctatgaa cgtggcaaca
     2641 cttttatgtt tggcgtcacg ctgcgcacca actttaacga tctgcgcccg tcttacaacg
     2701 ataacgcccg cccgcaatat caaccgcagc cgcaggatgc cattttgcag cattcggtgg
     2761 tggcgaatca gttaacgctg ttgaaataca atgccggact tgccgatccg cagatccagg
     2821 cgaaaggcga tacgctgtat gttaccggcg agcaggtgaa atatcgtgat ccgcgcgaag
     2881 ggatcatccg tgctaatcgg atcgtgatga acgatctgcc ggatgggata aaaacgatcc
     2941 gcattacgga aaatcgcctt aatatgccgc aggtgacgac ggaaaccgat gtcgccagcc
     3001 tgaaaaatca tctcgccgga gagccgttgg gccacgaaac gaagctggcg caaaaacgcg
     3061 tcgagccagt ggttccgaag tccaccgagc agggctggta tatcgacaaa tcacgctttg
     3121 atttccatat cgatccggtg ctgaaccagt cgatcggtgg cccggaaaac ttttacatgt
     3181 atcagctggg cgtgatggga acggcggatt tgtggctgac ggaccatctg ctgaccactg
     3241 gcagcctgtt tgctaacctt gccaacaact acgacaagtt taactacacc aatccaccgc
     3301 aggactcgca cttaccgcgc gtgcgtaccc atgtgcgcga gtatgtgcag aatgatgtct
     3361 atgtgaataa cctgcaagcc aactacttcc agcatctggg caacggcttc tacggtcagg
     3421 tctacggagg ttatctcgaa accatgtttg gcggcgtggg ggcagaagtg ttgtatcgcc
     3481 cgctggatag caactgggcg tttggtctgg atgccaacta cgttaaacag cgcgactggc
     3541 gtagcgcaaa agatatgatg aaattcaccg attacagcgt gaaaaccgga catctgaccg
     3601 cgtattggac gccatctttc gcccaggacg tgttagtgaa agcgagcgtc gggcagtatc
     3661 tggctgggga taaaggcggc acgctggaga tcgccaaacg ctttgatagc ggcgtggtag
     3721 tggggggcta tgccacgatc acgaatgttt cgaaagagga gtacggcgaa ggggacttca
     3781 ccaaaggcgt gtatgtctct gtaccgctgg atctcttctc gtctggcccg acacgcagcc
     3841 gtgcggcgat tggctggacg ccgctgacgc gtgacggtgg tcagcaactt gggcgtaagt
     3901 tccagttgta tgatatgacc agcgaccgta gcgtcaattt ccgctaagtc atgggaaagg
     3961 tgccagtttt cgcactcaca ggcaccttta ttcttaatgg cctgtcgagc catcaggaac
     4021 gcagttcacc cccggtttgc gatctggtgt gtaaaggcga aaacattctg tcttcctgtc
     4081 cgtcgatatc ggttgcaggt ctggttgcct gagcgactgg gccatcattg ggctggagac
     4141 attgctttgc ggtaggtcgg ctttatcagc agtaccgagc ggaccagcat aagcaggaag
     4201 aacagagact gataacatca aagcagcaaa ataaggcttc atttttacca cctttatcag
     4261 gttacgtttc atttgttcca gaggaatatt gtcgattttt cgcgcattgc tggtggctgg
     4321 gaatcacctg aatgggtgat ttttttgggc tgtgtgtttt gccagatgag acattgatgg
     4381 gagatgccca cttaattgag ctgaacaaaa catacacaaa aaatatagat ctccgtcaca
     4441 tttttgcgtt atacaggaag ctcgccactg tgaaggaggt actgctatga cgtcactctc
     4501 tcgtccgcgc gtggagttta tctccaccat tttgcagacc gtactcaatc ttggcctgtt
     4561 gtgcctcggc ctgattttgg ttgtcttcct cggcaaagaa acggtgcatc tggctgatgt
     4621 gctgttcgcg ccagaacaaa ccagtaaata tgagctggta gaagggctgg tggtttactt
     4681 tctctatttc gaatttatcg cgctgattgt gaagtacttt cagtccggtt ttcacttccc
     4741 gctacgttac tttgtctata ttgggatcac tgccattgtg cgcttgatca tcgtcgatca
     4801 taaatcgcca ctggatgtgt tgatctactc ggcagcgatc ctgttactgg tgatcaccct
     4861 gtggttgtgt aattcgaaac ggctgaagcg ggagtaaaaa aaggcgctcc gtggagcgcc
     4921 gaataacagt cacaagttgg gataacgtaa gttgagggtg cagcggcata acattggcag
     4981 aacaacatct ttaacctttc acaccacctg ccgtcaggcc gttgaccagc cagcgttgag
     5041 ccagcaagaa gacgatggtg atcggtaatg cagacatcac ggcagcggcg gcaaagtcac
     5101 cccacaggta gttttgcggg ttgaggtatt gctgcatccc cacggccagg gtgtagctgt
     5161 ttacgtcacg cagtaatagc gacgcgaccg gaacttcagt aatggcagcg ataaacgaca
     5221 ggatgaacac aaccgccaga atcggtactg acagcggcaa caggacaagg cggaaggcct
     5281 gccacggtgt cgcaccatcc agcgccgccg cttcttccag cgaactgtcg atggtttcga
     5341 aataaccttt gatggtccag acatgcagcg caatcccgcc cagatacgcg aaaattacgc
     5401 cgccgtgagt attcaggcca atgaatggaa tgtactcacc cagacgatca aacaacgcat
     5461 acaacgcgac cagtgaaagt actgccggga acatctggaa aatcagcatt cctttcagca
     5521 gcgtcgcttt gcctggaaag cgcatacggg cgaaagcgta ggcgcaggtg gtggagagcg
     5581 ccacaatgcc aatcgcggaa atcccggcga cctttaccga gttccacagc cacagcagta
     5641 ccgggaatgg cggtggcgta atgcgaccat cagcctgttc cacgctaaaa cctaacgcca
     5701 gtttccagtg atcccaggag atttgctccg ggatcagact accggtcgcg aagttcccct
     5761 gacgcagcga gatggcgacg accatcagca gcgggaacat aatcgccgcg ataaaaagta
     5821 gcagtagcag gtgagtaata aacaaacgtg ctttttgcga tttcggttgg accattgcca
     5881 tttttgttat ctcccttaat caaacttcat tcgcgtggct ttcaggttca ctatcgccag
     5941 cgcgcccacc agcaggaaga tcagcgtggc aattgctgct gccagaccga agtcctgacc
     6001 cccgccgcct tcaaaagcga tacggtaggt gtagttaaca agcaggtcgg tataaccggc
     6061 tggcgtggtc gtgccaagac gatccgggcc gccgttggtt aacagttgaa tcagcacgaa
     6121 gttgttaaag ttaaaggcga agctggcgat catcagcggc gtcagcggtt taatcagcag
     6181 cggcagcgta atcttaaaga agttctggaa tggacctgcg ccatccattg ctgaggcttc
     6241 atacaaatcg tccggaatcg ctttcagcaa gcccatgcag aggatcatca tgtacggata
     6301 acccagccag gtgttgacga taatcagcat cgtgcgggcg gtggtcggat cgctgaacca
     6361 ggcgggcttc acgccaaaca gcgcgctcaa catcatgttg atttcaccaa aactctggtt
     6421 aaacaaccct ttgaaaatca agattgaaat gaacgatggc accgcgtagg gcagaatcag
     6481 caggacgcga tagaccgctt tgccgcgcaa cgcttcccac tgcaccagac acgccagaac
     6541 catgccgacc gccaccgtta aaaagacagt gatcagcgag aacaccacgg tccagacgaa
     6601 aatggcgagg aacggtttct gaatgccttc gtcggtaaag acgcgggtaa agtttttcca
     6661 gccggtggtc acggtgtaac cggggcttag cttttcatca ccccagttac cgtcggcagt
     6721 gatggactgg taaaaaccaa tctggtcgtt tggacgatat tttacgccac tctgattatt
     6781 ggtcagcgtg ccgtcaccgt cgagtgtgta gagcggctgc gtgccagaaa actggcgcag
     6841 ggagctcatc atcactttgt tgccatccgg cagaatggcg gtaatgtcac tcagtgcctg
     6901 acgattctgg gtaattacgc gcagattcgc gcgttcgcct tcgggctggg cggtcgtttc
     6961 tttcagttgc agtttttgct tgccgccaaa tttaaaagcg tcggagaggt aatttttgcc
     7021 ggtttcgccg tcgctgagcg ccagttgcca ctcatcgccc gccggataaa gaccaaaatt
     7081 atagattttg cctgcttgcc aggagcgatc tagcaacact tcctgcgcac gttcaaaagt
     7141 cagctggtta gtgctgctgt agttggtgaa ggcaatggcg atggtgcaga ccagagggaa
     7201 gaggacgaat aatcccattc cagccattcc cgggtaaaca tagcgccagg cgtaggcttt
     7261 acgattggcg aaaatataca gccccgctga gctcaatatc agcgtggtaa tggcgaacag
     7321 gtattcccct tgtgcgtaca ttaaaacaac aaggtaaccc accagcaggc cgagcagacc
     7381 taacactgac catttcagcg cgtcgctttg ccaccaatgt ttctttttaa tgacatccat
     7441 ggggttcttc ctcattccag gacggataag gctttcatac cttatccgac aacaactgcc
     7501 tgatgcgacg ctgacgcgtc ttatcaggcc tacatacgtt tcaatttcgt aggccggata
     7561 aggcgtttac gccgcatccg gcatttcaca gcattacttg gtgatacgag tctgtgcatc
     7621 tttcagcgca gaatccacag tctgacgacc gctggcggcg ttgatcaccg cagtacgcac
     7681 ggcataccag aaagcggaca tctgcgggat gttcggcata atttcacctt tctgagcgtt
     7741 atccatggtg gcggcaatac gtggatcttt cgcaagttca tcctgatagg acttcagcgc
     7801 tactgcaccc agcggtttgt ctttattgac cgcttccaga ccttcgtcag tcagcagata
     7861 gttttcgagg aactctttcg ccagctcttt gttcggactg gcggcgttaa tacctgcgct
     7921 cagcacgcca acgaacggtt tagatggttg acctttgaag gtcggcagta ccgttacacc
     7981 ataattcact ttactggtgt cgatgttgga ccatgcccac gggccgttga tagtcatcgc
     8041 tgtttcgcct ttattaaagg cagcttctgc gatggagtaa tcggtgtctg cattcatgtg
     8101 tttgttttta atcagatcaa ccaggaaggt cagacccgct ttcgcgccag cgttatccac
     8161 gcctacgtct ttaatgtcgt acttgccgtt ttcatacttg aacgcataac ccccgtcagc
     8221 cgcaattagc ggccaggtga agtatggttc ttgcaggttg aacatcagcg cgctcttacc
     8281 tttcgctttc agttctttat ccagcgccgg gatctcttcc caggtttttg gcgggttcgg
     8341 tagcagatct ttgttataaa tcagtgataa cgcttcgaca gcgatcgggt aagcaatcag
     8401 cttgccgttg taacgtacgg catcccaggt aaacggatac agcttgtcct ggaacgcttt
     8461 gtccggggtg atttcagcca acaggccaga ttgagcgtaa ccaccaaagc ggtcgtgcgc
     8521 ccagaagata atgtcagggc catcgccagt tgccgcaacc tgagggaatt tctcttccag
     8581 tttatccgga tgctcaacgg tgactttaat tccggtatct gtctcgaatt tcttaccgac
     8641 ttcagcgaga ccgttatagc ctttatcgcc gttaatccag attaccagtt taccttcttc
     8701 gattttggcg agagccgagg cggaaaacat catcgtcgtt aatgcggata atgcgaggat
     8761 gcgtgcacct gtttttattt tcataatcta tggtccttgt tggtgaagtg ctcgtgaaaa
     8821 cacctaaacg gactctagtt tctttatacg gcaacctctt tccatcctcc ttgcccctac
     8881 gccccatcgt tgctttgtgt gatctctgtt acagaattgg cggtaatgtg gagatgcgca
     8941 cataaaatcg ccacgatttt tgcaagcaac atcacgaaat tccttacatg acctcggttt
     9001 agttcacaga agccgcgttc tcatcctccc gcctcctccc ccataaaaaa gccagggggt
     9061 ggaggattta agccatctcc tgatgacgca tagtcagccc atcatgaatg ttgctgtcga
     9121 tgacaggttg ttacaaaggg agaaaggcat ggcgagcgta cagctgcaaa atgtaacgaa
     9181 agcctggggc gaggtcgtgg tatcgaaaga tatcaatctc gatatccatg aaggtgaatt
     9241 cgtggtgttt gtcggaccgt ctggctgcgg taaatcgact ttactgcgca tgattgccgg
     9301 gcttgagacg atcaccagcg gcgacctgtt catcggtgag aaacggatga atgacactcc
     9361 gcctgcagaa cgcggcgttg gtatggtgtt tcagtcttac gcgctctatc cccacctgtc
     9421 agtagcagaa aacatgtcat ttggcctgaa actggctggc gcgaaaaaag aagtgattaa
     9481 ccagcgagtt aaccaggttg cggaagtgct gcaactggcg catttgctgg atcgcaaacc
     9541 gaaagcgctc tccggtggtc agcgtcagcg tgtggcgatt ggccgtacgc tggtggccga
     9601 gccaagcgta tttttgctcg atgaaccgct ctccaacctc gatgctgcac tgcgtgtgca
     9661 aatgcgtatc gaaatctccc gtctgcataa acgcctgggc cgcacaatga tttacgtcac
     9721 ccacgatcag gtcgaagcga tgacgctggc cgacaaaatc gtggtgctgg acgctggtcg
     9781 cgtggcgcag gttgggaaac cgctggagct gtatcactat ccggcagacc gttttgtcgc
     9841 cggatttatc ggttcgccaa agatgaactt cctgccagta aaagtgaccg ccaccgcaat
     9901 cgatcaagtg caggtggagc tgccgatgcc gaatcgtcag caagtctggc taccggtcga
     9961 aagccgtgat gtccaggtgg gagccaatat gtcactgggt attcgcccgg agcatctact
    10021 gccgagtgat atcgctgacg tcatccttga aggggaagtt caggtcgtcg agcaactcgg
    10081 caacgaaacg caaatccata tccagatccc ttccattcgt caaaacctgg tgtaccgcca
    10141 gaacgacgtg gtgttggtag aagaaggtgc cacattcgct atcggcctgc cgccagagcg
    10201 ttgccatctg ttccgtgagg atggcactgc atgtcgtcga ctgcataagg agccgggcgt
    10261 ttaagcaccc cacaaaacac acaaagcctg tcacaggtga tgtgaaaaaa gaaaagcaat
    10321 gactcaggag atagaatgat gattactctg cgcaaacttc ctctggcggt tgccgtcgca
    10381 gcgggcgtaa tgtctgctca ggcaatggct gttgatttcc acggctatgc acgttccggt
    10441 attggctgga caggtagcgg cggcgaacaa cagtgtttcc agactaccgg tgctcaaagt
    10501 aaattccgtc ttggcaacga atgtgaaact tatgctgaat taaaattggg tcaggaagtg
    10561 tggaaagagg gcgataagag cttctatttc gacactaacg tggcctattc cgtcgcgcaa
    10621 cagaatgact gggaagctac cgatccggct ttccgtgaag caaacgtgca gggtaaaaac
    10681 ctgatcgaat ggctgccagg ctccaccatc tgggcaggta agcgcttcta ccaacgtcat
    10741 gacgttcata tgatcgactt ctactactgg gatatttctg gtcctggtgc cggtctggaa
    10801 aacatcgatg ttggcttcgg taaactctct ctggcagcaa cccgctcctc tgaagcgggt
    10861 ggttcttcct ctttcgccag caacaatatt tatgactata ccaacgaaac cgcgaacgac
    10921 gttttcgatg tgcgtttagc gcagatggaa atcaacccgg gcggcacatt agaactgggt
    10981 gtcgactacg gtcgtgccaa cctgcgtgat aactatcgtc tggttgatgg cgcatcgaaa
    11041 gacggctggt tattcactgc tgaacatact cagagtgtcc tgaagggctt taacaagttt
    11101 gttgttcagt acgctactga ctcgatgacc tcgcagggta aaggtctgtc gcagggttct
    11161 ggcgtcgcgt ttgataacga aaaatttgcc tacaatatca ataacaacgg tcacatgctg
    11221 cgtatcctcg accacggtgc gatctccatg ggcgacaact gggacatgat gtacgtgggt
    11281 atgtaccagg atatcaactg ggataacgac aacggcacca agtggtggac cgtcggtatt
    11341 cgcccgatgt acaagtggac gccaatcatg agcaccgtga tggaaatcgg ctacgacaac
    11401 gtcgaatccc agcgcaccgg cgacaagaac aatcagtaca aaattaccct cgcacaacaa
    11461 tggcaggctg gcgacagcat ctggtcacgc ccggctattc gtgtcttcgc aacctacgcc
    11521 aagtgggatg agaaatgggg ttatgactac accggtagct cttcaaccaa cccatactac
    11581 ggcaaggctg tgtcggctga tttcaatggc ggtagcttcg gtcgtggcga cagcgacgag
    11641 tggaccttcg gtgcccagat ggaaatctgg tggtaatagc aaaacctggg ccggataagg
    11701 tgcttgcgcc gcatccggca accaatgcct gatgcgacgc tgttgcgtct tatcaggcct
    11761 acaaccgctg ccgaaagtag gccggataag gtgtttacgc cgcatccggc ataaaaacag
    11821 ctttaagggg cgaaagcccc tctgattatc gggtttagcg cgctattgcc tggctaccgc
    11881 tgagctccag attttgaggt gaaaacaatg aaaatgaata aaagtctcat cgctctctgt
    11941 ttatcagcag ggttgctggc aagcgcacct ggaatcagcc ttgccgatgt taactacgta
    12001 ccgcaaaaca ccagcgacgc gccagccatt ccatctgccg cgctgcaaca actcacctgg
    12061 acaccggtcg atcaatctaa aacccagact actcaactgt cgaccggcgg ccaacaactg
    12121 aacgtttccg gcatcagtgg tccggttgct gcgtacagcg tcccggcaaa cattggtgaa
    12181 ctgacactga cgctgaccag cgaagtgaac aaacaaacca gcgtatttgc gccgaacgtg
    12241 ctgattcttg atcagaacat gaccccatca gccttcttcc ccagcagtta tttcacctac
    12301 caggaacccg gcgtgatgag tgcagatcgt ctggaaggcg ttatgcgcct gacaccggca
    12361 ctggggcaac agaaactcta tgtactggtc ttcaccacgg aaaaagatct ccagcagaca
    12421 acccaactgc tcgacccggc aaaagcctat gccaaaggcg taggtaactc ggtgccggat
    12481 attcctgacc cgattgcccg tcacatcact gatggtttgc tgaaactgaa agtgaaaacg
    12541 aactccagct ccagcgtgtt ggtaggacca ctatttggtt cttccgctcc agctccggtt
    12601 acggtaggca acacggcggc accagctgtg gctgcacccg ctccggcacc ggtgaagaaa
    12661 agcgagccga tgctcaacga cacggaaagt tattttaata ccgcgatcaa aaacgctgtc
    12721 gcgaaaggtg atgttgataa ggcgttaaaa ctgcttgatg aagctgaacg cctggggtcg
    12781 acatctgccc gttccacctt tatcagcagt gtaaaaggca aggggtaatt acgccccaca
    12841 gtgctgattt tgcaacaact ggtgcgtctc ctggcgcacc tttttttctc ctcctgtact
    12901 attgttgcgt ttttgttgcg cattagttca cttaatttgc tttacatctc ccgtaaatat
    12961 ttttctgcga tacaatgcct ttacgttatg taacggagag ttcggcatgt cacaccccgc
    13021 gttaacgcaa ctgcgtgcgc tgcgctattt tacagagatc cctgccctgg agccgcaact
    13081 gctcgactgg ctgttgctgg aagattccat gacaaaacgt tttgaacagc agggaaaaac
    13141 ggtgagcgtg acgatgatcc gcgaagggtt tgtcgagcag aatgaaatcc ccgaagaact
    13201 gccactgctg ccgaaagagt ctcgttactg gttacgtgaa attttgttat gtgccgatgg
    13261 tgaaccgtgg cttgccggtc gtaccgtcgt tcctgtgtca acgttaagcg ggccggagct
    13321 ggcgttacaa aaattaggta aaacgccgtt aggacgctat ctgttcacat catcgacatt
    13381 aacccgggac tttattgaga taggccgtga tgccggactg tgggggcgac gttcccgcct
    13441 gcgattaagc ggtaaaccgc tgttgctaac agaactgttt ttaccggcgt caccgttgta
    13501 ctaagaggaa aaaaatatgg agtggagtct gacgcagaat aagctgctgg cgtttcatcg
    13561 cttaatgcgt acggataagc caattggcgc gttactgctg ctctggccaa cattatgggc
    13621 gttgtgggtg gcgacaccgg gcgttcccca gctctggatc ctggcggtgt ttgtcgcggg
    13681 cgtctggctg atgcgcgctg ctggttgcgt ggtgaatgat tatgctgacc gcaagtttga
    13741 tggtcatgtt aagcgcacgg cgaaccgacc acttcccagc ggcgcggtga cagagaaaga
    13801 agcgcgtacg ctgtttgtag tgctggtact gatttcattt ttactggtgc tgacgctgaa
    13861 tacgatgact attctgttgt caattgccgc gctggcgctg gcgtgggtat acccgtttat
    13921 gaagcggtat acccatctac cgcaagtggt gctgggcgcg gcgtttggct ggtcgattcc
    13981 aatggctttt gccgctgtga gtgagtcggt gccattgagt tgctggttaa tgttcctcgc
    14041 caatattctc tgggcggtgg cttacgacac gcagtatgcg atggttgacc gcgatgatga
    14101 tgtgaagatt ggcattaaat ccaccgcaat cctgttcggc caatacgata aattgattat
    14161 tggtattttg cagattggcg tactagcgct gatggcgatc atcggtgagt taaatggctt
    14221 aggctgggga tattactggt caattctggt agctggcgcg ctgtttgttt atcaacaaaa
    14281 actgattgcc aaccgcgagc gtgaagcctg ctttaaagca tttatgaata ataactatgt
    14341 tggtctggta ctgtttttag ggctggcaat gagttactgg catttctgat gatgtaaaaa
    14401 agccggatga tcatccggct ttcttctggg ttgcctgatg cgctgcgctt ctcaggccta
    14461 cacaacatat cgcaatttat tgaatttgca gatcatggta ggccgaataa ggcgtttgcg
    14521 tcgcatccgg caattctcac tgattaccct tcgccctgcg tcgcactctc aatcgtcaaa
    14581 cgcacgtctg atgtaatcaa ctccgccagc aactgatata ccttcatcgt ttctgccggt
    14641 tcggcgtcgc cgctatcgct gatataccct tcatcacgca gcgtcagcac cagagaactg
    14701 aacaccgcct tgtcgaagaa ttccggcgcg ttgataccgt gcagcacgga gagacgttgc
    14761 gcgacggtgc ggctctcttt ttccagcgta ccgcggttga tcgacgggtt ggcactcaac
    14821 agccagaagg tgatggcata acgttgcagc gtttcgcgcg cgcctgcggc cagcagctgt
    14881 agcgtgcgag aatgcgccgg gttgatatgc aactcatcat cttgcagggt aatcagcccc
    14941 tgacgttgca tctcatttgc cagcgcatca ataacgtccg gtaactcgtc gcgatcccag
    15001 cgcaggaaca gttccgcttt cagcattggg taaagcacat tgacgtgctc catcaatacg
    15061 tcgcgggaga tgtggcgatg ctgggtgaca attgccgcca tcagcgaagg cagcaccaac
    15121 atatgcgcaa tgttgttgcg atagtaggtc atcagcaccg cttgctcgcg cggcagaatg
    15181 atgatgtcgc cgatagtgtc tttctcgact tcaaacttgt tcatttgcag cgcgtgatcg
    15241 ataagctcgc tggcgctggc tgaaggaacg gtagagtccg tggagtaagg cacgttgcgc
    15301 atcagatcca ggtagcagtt gagttgctcg gttaactgct cgcgggtgag tgagcgctga
    15361 cgtgacgcca gaagcgcagt acagcacagg ttcatggcgt tcgccgcgcc tgcgttgtta
    15421 atgcgtacca tcagatcggc agcaatatta ttgaccgtcg gcgttaacca ggccggacgc
    15481 accgcttcga tgggatcgat agattcacgc cagtctggta cgtgctggtt aaggtaggtc
    15541 atcaacggca ttggttcacc gaagttgacg taaccctgac cgagattacg cagcttgctt
    15601 aaaccgcgca gcatctgcgg caggctctct ttctctttcg tcgcaccgcg cagttctttg
    15661 gcgtaagtac ccacttccat gacgtgctca taaccgatat agatcggaat cagcgtaatc
    15721 ggacgagtgc cgccacgcag catcgcctga atggtcatcg atagcgtacc ggttttcgga
    15781 tccagcaaac gccccgtacg ggaacgaccg ccttccacga aatactcgac ggaataacca
    15841 cggctgaaca gttcgccgag atactcacgg aaaacggtgg aataaagttt attgccttta
    15901 aacgtacggc gaataaagaa cgcccccaga cggcggaaaa tcggcccggc cggccagaag
    15961 ttcaggttga tcccggcggc gatatgcggc ggcaccagcc cctggtgata cagcacgtaa
    16021 gaaagcagca ggtagtccat gtgactgcgg tggcaaggca catacactag ctcatggccg
    16081 tcgtgggcca gctggcgaac gcgctcggcg ttatggacgt tgataccctg gtaaagtcgg
    16141 ttccaggtga agcccagaat gcggtcagtc aggcggatca tctcgtaaga gaaattcgcc
    16201 gcaatctctt ccatcagcgc aatcgcgttc tgctgcgctt tttcatggga gatttttttg
    16261 ctgcgtgctt catcttctac cgctttcgca atggcgcggg aggcgagcag cttattaaac
    16321 agatcctgac gagcaggcag acgtggaccc acggcagcca gacgttgacg ggcaaagtgc
    16381 atacgcgcca cgcgcgccag tttctgagcg atagttttat ccgtgccgtg ttcatccgcc
    16441 atacggcgca gtgaaactga cggcgagaaa cgcacaaaac tgtcgcgacc gagccacagt
    16501 acagcgaaaa atttctgtac gccgttaagc atacgcagcg gcgggttcac ttcgcctttt
    16561 tcacgccccg gcgcgcgacc aaacatcacc gatactggca ccatctgcac atccagattt
    16621 gggttgctgc ggtgcaaatc gagatagtcg tggaacagct taatagactc ttctttcggc
    16681 gtgtaatagg tgaacacacg cggcccgccg tgaatgaaca catagcgcgg cagtagcgtg
    16741 ccgtcgattt ccagcggttc taacgggtca ggcaggtcat gtgccagaca ctgggcgcgc
    16801 aacgtcagca agtcagcttt cgagttgtac ggtaaaacgt acataattgg acgagaggta
    16861 tccagcccca gttccggggc aggatccgcc ggaatagact tgctttttac caggatgctt
    16921 aatggtaaat tcagtaattt gtagtaaatt cgtggccagc cggacataaa cgatgtaaag
    16981 cctctggtta ataatgcaaa tgcgcggcaa ggatagcaga aagtcatggg aaattctgtg
    17041 gtatccgctc atgtttcgcg cggcgttacg caaacccgaa tcatcggatt taacggtaca
    17101 ctgatattga cgctcataat gtaaaaaggt tctttcaatg gccaataata ccactggatt
    17161 catccgaatt attaaagctg ctggctattc ctggaaaggt ttacgcgctg catggatcaa
    17221 cgaagcggca ttccgtcagg aaggcgtagc ggtattgtta gcggtggtta tcgcctgctg
    17281 gctggatgtg gacgcgatta cccgcgtgct gcttatcagc tccgtgatgc tggtgatgat
    17341 tgtggaaatt ctcaatagcg ccatcgaagc tgtggttgac cgaattggct ctgaatacca
    17401 tgagctttcc ggacgcgcta aagatatggg atctgccgca gtgctgattg ccattatcgt
    17461 cgccgtgatt acctggtgca tcctgttatg gtcgcatttt ggataaccct tccagatttc
    17521 gataaatctc tggtttattg tgcagtttat ggttccaaaa tcgccttttg ctgtatatac
    17581 tcacagcata actgtatata cacccagggg gcggaatgaa agcgttaacg gccaggcaac
    17641 aagaggtgtt tgatctcatc cgtgatcaca tcagccagac aggtatgccg ccgacgcgtg
    17701 cggaaatcgc gcagcgtttg gggttccgtt ccccaaacgc ggctgaagaa catctgaagg
    17761 cgctggcacg caaaggcgtt attgaaattg tttccggcgc atcacgcggg attcgtttat
    17821 tgcaggaaga ggaagaaggg ttgccgctgg taggtcgtgt ggctgccggt gaaccgcttc
    17881 tggcgcaaca gcatattgaa ggtcattatc aggtcgatcc ttccttgttc aagccgaatg
    17941 ctgatttcct gctgcgtgtc agcgggatgt cgatgaaaga tatcggcatt atggatggcg
    18001 atttgctggc agtgcataaa actcaggatg tacgtaacgg tcaggtcgtt gtcgcacgta
    18061 ttgatgacga agttaccgtt aaacgcctga aaaaacaggg caataaagtc gaactgttgc
    18121 cagaaaacag cgagtttaaa ccaattgtcg ttgaccttcg tcagcagagc ttcaccattg
    18181 aggggctggc ggttggggtt attcgcaacg gcgactggct gtaacatatc tctgagaccg
    18241 cgatgccgcc tggcgtcgcg gtttgttttt catctctctt catcaggctt gtctgcatgg
    18301 cattcctcac ttcatctgat aaagcactct ggcatctcgc cttacccatg attttctcca
    18361 atatcaccgt tccgttgctg ggactggtcg atacggcggt aattggtcat cttgatagtc
    18421 cggtttattt aggcggcgtg gcggttggtg caacggcgac cagctttctc tttatgttgt
    18481 tgctgttttt acgcatgagc accaccgggc tgactgcaca ggcttatggt gccaaaaatc
    18541 ctcaggcatt agcccgtgcg ctggtgcaac cgttgctgtt ggcgttgggg gccggggcgt
    18601 taattgcgct gctgcgtacg ccgattatcg atctggcgct gcatattgtt ggtggtagtg
    18661 aagcagtcct ggaacaggcg cgacgctttc ttgaaatccg ctggttaagc gcaccggcgt
    18721 cgctggcgaa tctggtatta cttggctggt tactcggcgt gcaatatgcc cgtgcgccag
    18781 taattttgtt agtggtcggc aatatcctca acattgtgct ggatgtctgg ctggtgatgg
    18841 ggctgcatat gaacgtgcag ggcgcggctc tggcgacggt tattgcggaa tatgcaacat
    18901 tgctgattgg tctgctaatg gtgcgtaaaa ttctcaaact acgcggaatc tccggcgaaa
    18961 tgctgaaaac agcctggcga ggaaacttcc gtcacttgct ggcgcttaac cgcgatatca
    19021 tgctgcgctc gctgttgctg caactctgtt tcggcgcgat cactgtactt ggcgcgcgac
    19081 tggggagtga cattatcgct gttaacgcgg ttctgatgac gctactcacc tttaccgcct
    19141 atgcgctgga tggttttgcc tacgcggttg aagcacattc cggtcaggca tacggtgcgc
    19201 gcgacggtag tcaattgctg gatgtctggc gggcagcgtg ccgccagtcg gggatcgtag
    19261 cgttactgtt ttcggtggtt tatttgctgg caggggaaca catcattgcg ttgctgacgt
    19321 cgttaaccca gattcagcag ctggctgacc gctatcttat ctggcaggtg attttgccgt
    19381 tggtcggcgt ctggtgttat ctgctggacg gcatgtttat aggcgcaacg cgtgccgccg
    19441 aaatgcgtaa cagtatggcg gtggccgccg caggttttgc actgacgctc cttacgctgc
    19501 cgtggctggg gaatcatggt ttgtggctgg cgttaaccgt ctttctggcg ttacgcgggc
    19561 tttctctggc ggctatctgg cggcgtcact ggcgtaacga tacctggttt gtcgcaacgt
    19621 gacggttaaa aattctgaat aaataatcct aagccaaatt gctgactaca cttaatctca
    19681 cgttcagaag aaaagtgaac gtactctcat tcacaaccta acgatgaggt cttgattatg
    19741 aataaagatg aagccggcgg taactggaaa cagtttaaag gtaaagtgaa agagcaatgg
    19801 ggcaaactga ccgatgatga tatgacgatc attgaaggaa aacgtgatca gttagtgggt
    19861 aaaatccagg aacgctacgg ttatcagaaa gatcaggcag aaaaagaggt tgatagttgg
    19921 gaaaaacgcc atgattaccg ctggtaatta atccctcctg cccgacatgt acaaggatgt
    19981 acaccctttt agcgaggttt ctttttcacc tgaatagagt ggtcatgatg gcactgttca
    20041 ggatgacgac acgcttctac ttctacacat gctgaacata atccatgtgc ttcaataaca
    20101 ttatgccgca gggcaaaacc catttttgcc gccagcgtat gcatgatgtc ttccacgcct
    20161 tcagcacatt cttctttcac cgcgccgcag cgatcgcaaa taaacatggc tgaagtatgg
    20221 gtgggctgat cgaacagatg acagagcaca taactgttgg tggattccac cttatgcaca
    20281 aaaccttgtt caagcagaaa ctccagcgcg cgataaaccg ttggcggctt ggcttgcggt
    20341 tcagcttcgc gcagcaaatc aagcagatca taagcgctga tagcgccatc ctgcagactc
    20401 atcaggcgca acacttcgag acgctgtggg gtcaggcgca cattacgctg tgcgcagatt
    20461 ttttcagcct gcgctaataa ctcctgcgtt gtggtctttt ccattgggca cctcgacagt
    20521 ggggttaaga atgtccttac tttaccatag ttctcactta aacgccgaga acagggtgtc
    20581 atggctgtca cattgcctga tcgcatattc gttatgctat agtagcgccc ccaatttacc
    20641 agatgcttaa aaagtcgcca tgccagcaga atcacacact gtttatcccg cttaccgttt
    20701 ctcgattgcc ccgatgcttg actggacgga cagacattgc cgttatttcc tgcgtctgct
    20761 ttcccgcaat acgttgctgt ataccgaaat ggtgaccaca ggggcgatta ttcacggtaa
    20821 aggtgattac ctggcgtaca gtgaagaaga acatccggta gcgttgcaac tcgggggtag
    20881 cgatccggcg gcgctggcac agtgtgcgaa gctggcagaa gcgcgcggat atgatgagat
    20941 caacctgaat gtcggctgcc cgtctgaccg ggtgcagaac ggcatgtttg gtgcgtgtct
    21001 gatgggtaat gcgcagctgg ttgccgactg cgtgaaagcg atgcgcgatg tggtgtcgat
    21061 tccggtgacg gtgaaaacgc gtattggcat cgatgatcag gacagctatg aatttctctg
    21121 cgatttcatc aacaccgttt ccggcaaagg tgagtgtgag atgttcatca tccacgcacg
    21181 taaagcctgg ctttctgggt taagtccgaa agaaaaccgt gagatcccgc cgctcgatta
    21241 tccgcgtgtg tatcaactga agcgtgactt tccgcatctg acgatgtcga ttaacggtgg
    21301 tatcaagtcg ctggaagaag ctaaagcgca tttgcaacat atggatggcg tgatggtcgg
    21361 gcgcgaggcg tatcagaatc cggggattct ggcggcggta gaccgagaga tttttggttc
    21421 ctcggatacc gatgccgatc cggtggcggt agtgcgcgcc atgtatccgt acattgagcg
    21481 tgaactcagc caggggacgt atctcggcca tattacccgg catatgctgg gcttgttcca
    21541 gggtattcct ggcgcgcggc agtggcgacg ttatttaagt gaaaatgccc ataaagcggg
    21601 tgcagacatt aatgtgctgg aacacgcgct caaactggtg gcggataagc gttaactttt
    21661 caccaaaaag tagtcaaatt caccacgccc tgcgcaccgt cgcggggcgt tttgctgtta
    21721 aatcaataga ttatttttgg catgattctt gtaatgccag caagagattt catatttggg
    21781 agagcatcat gctggaacta ctttttgtaa ttggcttttt tgtcatgctg atggtcactg
    21841 gtgtttcgtt gctgggtatt atcgccgcgc tgattgtggc gacggccttt atgttcctcg
    21901 gcggtatgct ggcattgatg attaagttgc tgccgtggct gctgttggcg gttgcggtgg
    21961 tgtgggtgat cagggcgatt aaagcaccaa aagtgccgaa atatcagcat tatgaccgct
    22021 ggcgttacta agggattgtg cggatgatca caacctaagg ttttatcctt agaacaaaat
    22081 aggaattgat aatcaaatct gtcactattg cgtgtcaaac aaattcatcg agctgtaccc
    22141 tacatacagc cgaactataa aagaaagggc ttcccaggtg gaagcccaat ttcttttatg
    22201 gaatcaacag gctggaaccc tgcgtcgccc ggctttccag aatctcatgc gcacgccgcg
    22261 catccttcag cggatatttc tgctgctcgg cgacatcgac cttaatcaca ccgctggcaa
    22321 tcaaagagaa cagttcatta ctggcctcgg ttaattcctc ccgcgtggtg atatagcctt
    22381 gcagggaagg gcgtgtcaca tacaacgagc ctttctgatt gagaatgcct aagttcacac
    22441 cggtaaccgc acctgatgag ttgccaaaac tcaccattaa gccgcggcgt tgcaggcaat
    22501 ccagcgaccg ttcccaggtg tctctgccca ctgaatcgta caccacgcgc actttcttgc
    22561 caccggtgat ctcttttaac cgctcgacca gattctcttc acgatagtta ataacctgcc
    22621 atgcgcccgc ttttagcgca ctctgcgctt tttgcgcggt tcccacggtg ccgataagtt
    22681 tcgcgcccag ggcttttgcc cactggcagg caatcaagcc aacgccgcca gccgctgcgt
    22741 ggaacaggaa ctgttcatcg ggtttaattt cataggtttt gcgcagcaga taataaaccg
    22801 ttaagccttt caggaaggat gccgcagctt gctcaaaaga aattgccgca ggcagaatcg
    22861 ccgctttatc cgcattaatg ttatgcatag agctgtaagc gcctaatgcc gattgtgcat
    22921 agactacgcg atcgcctgcc ttaatatgct ttacaccact gccgacttta ctcacgatgc
    22981 ctgccgcttc ggtgcctaat ccgctgggta gcgatggtgg cgggtaaagg ccgctgcgga
    23041 tatacgtgtc gataaaattg atgccgatgg ctttattttt gacctggatt tcattctccg
    23101 ccggatcggc aggagtgaac tccacggcct gaagtacttc cgggccaccg tgcttgtgaa
    23161 attcgatgcg cgttgccatt taaactcctt tactgagcga catatctgtt ttaagcctaa
    23221 cagtagtcgc caggccgcgc ggggattttt tccgggctgc ggtcacaacc cggaatatct
    23281 ctgatcagta ttgaaacggc gtcgccgcaa tccagttttg ccactggcgc tcaaagttgg
    23341 ttagcgttaa tacgccccgt tcacggcacg ggcgagtcca ggtttggtaa tcattagctt
    23401 cccgacaaac acaaatcata tattgcctgg catcctgatt ttctggccga aaacgccagg
    23461 ccaggagtgg cccacctggt gtttgcggtg caataccggc ttcgccatca tagatagccc
    23521 tggcaccacg actcccacca ccgttttcga cataacattg taaagaacgc agtacagcca
    23581 gcgccagttg cgcactttgc cgccactgga aagattgtgc caggctgttt tcatcgcatt
    23641 gaataccttc ccggcgaata tcttcacaaa gtgttgccag ggtttgcgcg gcagtgttga
    23701 tcccctgtgt atgacacaaa attgcggcat ggtggctcat ggtattttgt atcgtttctc
    23761 gcacgtcgtc acgtagcagc ccctgattca ggtactgttg cgcctcatta atcgtactga
    23821 gaatttgttg cctctcgaga ggccggtcct ggcttttctg atgagcaata tgtaatgcac
    23881 agcggcgggc gaaaacttgc ccggcattga gcgctgctcc acctgggcgc gtgacaccat
    23941 gtgttccggc attttctccg gcagcataac aaccagttag cgatgtacgt ccccaaatat
    24001 caacttcaat tccgccatta agatgttgat tatttagcgt aaacttgagc ggtgaggctg
    24061 tgagatcatg accgtgcatc tgatacaaac gaatagccag aggattcatc tgtgttaatc
    24121 tcgccagcgg ttgtgccaac agggcgtgat tttgttgcag atagtcgatg acatcatcat
    24181 ccagttcttg caggttgaat gacgttccgt ccgctgcggg ttctggttcc cgtagaaaat
    24241 caagaaaaac atcgcgccct ttttgagtct cttcataaac tgcgacatcc agcaggctgg
    24301 agccatattc cagcattcgt tccgcgtgaa acggccactg gtaaccttta cgaaatattg
    24361 cggaggccag catacgagtc gtggggtaat aagctgccag aaaattatac tgacgcccgg
    24421 agtggtcctg cgaatagatg cgcggcatcg cctgcatgta agtaccggaa agattccacg
    24481 gaaactggct gcgcggtgta ccgataccaa actgactttc cgtaagatta accagagtta
    24541 tacctgcttc cagtgccatg cccaacgcac taaaacagtt caccggataa acactgtcac
    24601 gataaagttc acctggtccg cccgtggcta aaaccagatg ctggcagcga atcacgacca
    24661 ttcgccaggg attatcccga caggctttat cgatggcgat cacccccacg actcgtcgat
    24721 tttccccttc accactggtc aggatatgaa tcgccgttgt gtgatcgcaa agaggaatat
    24781 taaggcgcat tgcctcctgg gccagcacct tcaccatcag gcgcgatgtt cgaggaccgc
    24841 agctagtggc acggccaaat tcatcgtgat cggtttgata acgcaacgtt gcgccaaaga
    24901 gatcttcagg cagatccaga cccagatatt tcagtacttc acaggtgttc accgatccga
    24961 ccgcctcaac atatgcggtg tcatgatcca tcgcaccacc tgccgccagg gtgttactta
    25021 atgtcagaaa atggtcacca tttgcacgcg tattcgcggt gtgaagcgtt tgtttatcgg
    25081 aaccagaaca ggcggaagtg ccccagaagg ctttgctgga ggcgaccatt acatcgacat
    25141 tttgccgttt cagctcgacg gcagctcgcc atcctgccgc gccactgccc agaaccagcg
    25201 tgttgcagca gtactctggt aaaatggtac cgttaaggtc gatactaccg ctggcttgag
    25261 ccagcggtgc gtaatggggc attttgatat cagtgagcgc cgttagcgtt tcgtcaatca
    25321 ttgcgtccca cctatttcat cagactttcg attaagccgc tcattgccat atagcccatg
    25381 attagcgtaa atacgcttat ggcaataaac atgacgtttt cccagcgtga gcaacgatat
    25441 ttgcccatta acgaaggctt gttcgccagc cagaacatac ataaaacagt cagcggcaga
    25501 ataatggcgt taaaggcctg gctggcaatc atcaccaata ccgggcgtgc gtggaacagc
    25561 ggcacaacca gacaaagcag agacataacc gtcatcagga tacggtaacc tgggctaact
    25621 tcgttgctgg tatcacgcca gtcagccagc agccagggcg ttaacagcaa atttgggaac
    25681 tggctggaaa gcccggctgc aacaatacca ataacaaaga gccccacggc agctgagcca
    25741 gcaataggtt cgagaataat gaccatttgc gaggcattat ttaaacctgt gccagtttgg
    25801 tacaacgtac ccgcagccgc agccatcacc gccgcactga tgatgaacat cattcctgca
    25861 ctgacaatgg catcacgatt ttgatgaggc aaattgtcca caccccaacc attacttttt
    25921 accagggtcg tgcgcataag aaacattgct ggtgctacgg tggtacccac catgccagaa
    25981 attatcagaa atggcgtact gttatcgcca gcgacaggat cgggaacaga cggaataaat
    26041 ccggccagca gatctgcgac cggcggcatc ataatgaaaa aattaatcac aaacgccagc
    26101 cccataatgc caaccagcgc ggagaggatt ttgtcgaacc cggcgttgct gttggtccag
    26161 agcatcacgt taatcataat gaccatcgcg atagcccagg cgagagctga gataccgcca
    26221 ctaaaccagg tcgtggacca tgttgccatt acatccgcca gaatacccat tacccccata
    26281 atggaaccgc tcacggcaac ggttaacccg atcagaaaca acatcgcaac gccgggatgg
    26341 atatggcgac gaaatgcagt caacgcgcgt tcaccagtga cggaagcata acgaccataa
    26401 atacggatta aaaaccaggt aacaatgcaa gaaagcgcca cggcccacat gagcgagaca
    26461 ccatagttag cacctgcttt agccatagtg gtgatgctgc ctgttccgat gctgtagcca
    26521 ataagaaaga tgccaggtgc aaaggccgcg agttttgtca ttattcgccg aaataaagaa
    26581 ggcgtttggg gaacagaggt tgtcatattc attatctttc tttccttgta agaggcgatc
    26641 agcgtgcctg aaagtgcggt tgacgcttct cagtaaatgc ctgactgcct tcatgagcat
    26701 cttcagtcgt ggcggaaaaa gcgccagcca gtgcttcaag cgtcgaggcc acagcaatac
    26761 cttctccggc atcaataacc tgcttggcga tctgcactgc aacgggagca cgctgggcga
    26821 tacgttgtgc cagttgcagt ccggcgtcgt gtaacgcctc cctggcgaca acgcgattca
    26881 ccagcccgca ggacagtgcc cgctgtgcat ccagtggttc accggtaaat accagttctt
    26941 tcaccagtga gcgcccaagc agtgccgtca gccgctgcgt tcctgaccag ccaggcactg
    27001 tcgccagccc ggtttctggt agtgccattt gtgcttcggc gctacaaata cggatatcgg
    27061 cggtagcagc cagttccaga ccaccgccat aagccaggcc gtgcaacagg gcgattacgg
    27121 gctgtggcag ttgtgccaga gcatcaaatg cctgatgacc gcggcggatc cagttgcgcc
    27181 acatatcgag tgcagaaaga gaggtccagc gatgaatatc cgcgccgaca caaaacgctt
    27241 tttcactggc actggtcagc agtactacgc ggatctccga atcagtttcc aacgtgtggc
    27301 aatgcgccag cagttgttcg agcatctctg gggttaatgc gttgagtttc gccgggcgat
    27361 taatcaccaa agtggcaatg aaaccgttgc gctggaggtc aatggtgccg ttattcatgg
    27421 tgacttcctt gttgaaaata gaggggattc attagtttga gatcgtctgc cagacggagt
    27481 ggggcatcgc tttgggcgag aatgtcgcgt tcaggatcaa tacctggggc aatttctgtg
    27541 accagtagcc cgtctggggt taattccaga acgcagcgtt cagtaatgta ctgtacttgt
    27601 tgcccgagtc ttattgcgcg tttgccggag aacgtcacgt gggcgacgtc ctgtaccagt
    27661 tttttggctt tgccttcttt aatgatacgc agctgaccct cttccagttg cagttgggca
    27721 ccggcattga aaaagccaga gaaaataatg cgtttcgcat gtgaggtgat atcaataaat
    27781 ccaccgcatc cggcagtgac atgtggacga gcggggagat gtgagacgtt cactgagcca
    27841 tctgcgccga tttgtaaaaa cgacatcaga gtgagatcaa aaccaccacc ctggaaatag
    27901 gtgaactgct ggggagaggg caaaaatgct tctgcattag aggcacaacc gaactgaaat
    27961 tccagcagtg gaactccgcc aatcgcgccc tgttccagca gccaggtgac gtcaccgtgg
    28021 cgaccttgtt ccagtaaaat gcgtggtacg ttggcagaaa taccaaagcc aatatttacc
    28081 gcatctccac tttgtagttc ttgcgcgact cgctgggcaa taactcgcgc cgggccatgt
    28141 tccatataac gaaaggctga cagcggacga ctgatttcgc cggaaatggc aggttcatat
    28201 tgcgtttgtg tcgtttgtgt ttgttccggc gcaatcacga tgtaatccac cagtacaccc
    28261 ggaatacgca cctcttttgg tttcagactt ccggcggcaa cctgtcgttt tacttgcgca
    28321 attatgatgc caccattatt gcgaacggca gtagcctgtt ccagtggccc cagataagcc
    28381 ccttcatgtt cataagtcag gttgccgcgt tcatcggcag tggtagcgcg aatgatcgcc
    28441 acctgcggca cgatactggg gaaaaacagc cagtcgtcgt tagcaaatcg aacacgttca
    28501 accagagatt gttgcgccgc tttttcattc attgcaccgc cctgcaacag gggatccaca
    28561 taggtatcca tgcctgtttg tgtcagcacg ccaggacgat gagccgccgc ttcccggtgc
    28621 atatcaaata agatcccgga aggcagattc caggcgggga tcgcgttgtc attgatcatt
    28681 tgccagatta acggaggcgt cattgatgat ggaccggagg ggtaggagcc agcgatcact
    28741 tttgccagta agcccggttg cgccagatga tcgatgcctt ttacgccata catatcacca
    28801 gcggcgatag ggtggatgga ggtgatgttt tgcggatgtc cggcacgttg aaagcgttca
    28861 cctattgctg ccagcaccgc atccggacac cccattccac tggaggagga gacggtgaca
    28921 actgcgccat caacaatcaa atctgcagct tccggtgcag tgatcgtgcg tacagagcac
    28981 ataataattt ccttgtttcg atataaaaat aattaaaaat atacatttaa aacaatatga
    29041 taattttcat aaattaattg gaacgatcta atttatgcgt aatttgtacg attagggtgt
    29101 aaaaaacaca acaagggaag tgcgaatctg tgatcgaaac gacgatctcc tgcgatccct
    29161 tgcgttaacg gatcgcatga gtaaaaggag attatgataa agtaattgcc ttgatcttat
    29221 gggggtttgg agcaggcatg gcgaagtcgg atacaggaag aaagcgggta acattaaccg
    29281 atgttgcgcg tgcggctggc gtttcgaaat cgactgtatc gctggttctc aacgacagtt
    29341 cacttatcaa aaaagagacg caacaaaaag tccagcaagc cattgaacaa ttaggctatg
    29401 tctataaccg ttttgctgcc aatctgcgtt cgcaaaaatc gctaactatc ggcgtagtca
    29461 ttgacgattt gataaatcct ttcttcgctg aatttactat gggtcttgag atgacgctgg
    29521 cggagcacgg ttttatcacc gtgatgtcca atacctccca gcgtagtgac cggcaaaaac
    29581 aggtgcttga taccttgctg gaacaccacg tcgcgggcat tgtcctctgt ccagtgaaca
    29641 gtacctcaga agccgattta cagcgttatg ccaacagttc cacaccctta ttaatcacga
    29701 tgcgccctct ggattggcaa caattgcccg tcgattacgt tggcgtggac agccacgcag
    29761 gtgtacggga agcgacggaa tatctcattc aacaagggca cagagatatc gtgtttattg
    29821 gtggtcttac gcaccatatg cgttatcagg gataccttga ggcgatgaat caccatggtt
    29881 tacagccgtg gagtactgat gctttttcgc tgcgtgctga accgactcag gccaacggct
    29941 accagttgat gcaacagctg ttggatatgc cttcgccacc cacagcggtg atttgttata
    30001 acgatttaat ggcttttggc gcggagtcgg cactaggcga aagaggatta tttgctgggg
    30061 aagatatttc gttgattggt aatgatggcg tggcggcttg tgcttacagt aacccgccgt
    30121 taaccacaat tgcggtagaa ccattggctc tggggaaaca ggcggcgcag caaattttac
    30181 ggcgtattgc gcaaccagac gcaccgctca gccattacat ctatcgcccg accctgcaaa
    30241 ttcgcgcttc caccggaaag cgggggcgtt aaatgctgta gcgagaaact gtttctcgct
    30301 atactctctt gtcccccatt caacagcagg taacaccatt cgttatggca ggcaatagac
    30361 ctttcaacaa acaacagact gataaccgcg aacgcgatcc acaagttgcc gggctgaaag
    30421 tgcctccgca ctcgatcgaa gcggagcagt cggtgttggg cggtttaatg ctggataacg
    30481 aacgctggga tgatgtagcc gagcgtgtgg tggcagacga tttttacacc cgcccacacc
    30541 gtcatatctt tactgaaatg gcgcgtttgc aggaaagcgg tagtcctatc gatctgatta
    30601 cccttgcgga atcgctggaa cgccaggggc aactcgatag cgtcggtggt tttgcttatc
    30661 tggcagagct gtcaaaaaat acgccaagtg cggcgaacat cagtgcctat gcggatatcg
    30721 tgcgcgaacg tgccgttgtc cgtgagatga tctccgttgc gaatgagatt gccgaagccg
    30781 gttttgatcc acaagggcgt accagtgaag atctgctgga ccttgctgaa tcccgcgtct
    30841 tcaaaattgc cgaaagtcgt gcaaacaaag acgaagggcc gaagaacatc gccgatgtgc
    30901 tcgacgcaac cgtggcgcgt attgagcagt tgtttcagca gccacacgat ggcgttaccg
    30961 gggtaaacac cggttatgac gatctcaaca aaaaaaccgc tggcttgcag ccgtcggatt
    31021 tgatcatcgt tgccgcgcgt ccgtcgatgg gtaaaacgac atttgcgatg aacctcgtcg
    31081 aaaacgcggc gatgttgcag gataaaccgg tacttatctt ctcgctggag atgccatcag
    31141 aacagattat gatgcgttct ctggcgtcgc tgtcgcgtgt tgaccagact aaaatccgta
    31201 ccgggcagct cgatgatgaa gattgggcac gcatttccgg caccatgggt attttgctcg
    31261 aaaaacgcaa tatctatatc gatgattcct ccggcctgac gccaacggaa gtgcgttccc
    31321 gcgcacgccg tattgcccgt gaacacggcg gcatcgggct tatcatgatc gactacctgc
    31381 aactgatgcg cgtaccggcg ctttccgata accgtacgct ggaaattgca gaaatctccc
    31441 gctcgctgaa agcactggcg aaagaactga acgtgccggt ggtggcgctg tcccagttga
    31501 accgttctct ggaacaacgt gccgacaaac gcccggtcaa ctccgacctg cgtgaatctg
    31561 gctctatcga gcaggatgcg gacttgatca tgtttatcta tcgtgatgag gtgtatcacg
    31621 aaaacagtga tttaaaaggc atcgcggaaa ttattatcgg taaacaacgt aacggcccaa
    31681 tcgggacggt acgcctgacc tttaacggtc aatggtcgcg cttcgacaac tatgcggggc
    31741 cgcagtacga cgacgaataa taattatttt atgaattagg taattaaagc aaacacttat
    31801 caaggaacac aaatgcaagc ggcaactgtt gtgattaacc gccgcgctct gcgacacaac
    31861 ctgcaacgtc ttcgtgaact ggcacctgcc agtaaaatgg ttgcggtggt gaaagcgaac
    31921 gcttatggtc acggtcttct tgagaccgcg cgaacgctcc ccgatgctga cgcctttggc
    31981 gtagcccgtc tcgaagaagc tctgcgactg cgtgcggggg gaatcaccaa acctgtactt
    32041 ttactcgaag gcttttttga tgccagagat ctaccgacga tttctgcgca acattttcat
    32101 accgccgtgc ataacgaaga acagcttgct gcgctggaag aggctaacct ggacgagccg
    32161 gttaccgtct ggatgaaact cgataccggt atgcaccgtc tgggcgtaag gccggaacag
    32221 gctgaggcgt tttatcatcg tctgacccag tgcaaaaacg ttcgtcagcc ggtgaatatc
    32281 gtcagccatt ttgcgcgcgc ggatgaacca aaatgtggcg caaccgagaa gcaactcgct
    32341 atctttaata ccttttgcga aggcaagcct ggtcaacgtt ccattgccgc gtcgggtggc
    32401 attttactct ggccacagtc gcattttgac tgggtgcgcc cgggcatcat tctttacggc
    32461 gtatctccgc tggaagatcg ctccaccggt gccgattttg gctgtcagcc agtgatgtcg
    32521 ctaacctcca gcctgattgc cgtgcgtgag cataaagccg gagagcctgt cggttatggt
    32581 ggaacctggg taagcgaacg tgatactcgt cttggcgtag tcgcgatggg ctatggcgat
    32641 ggttatccgc gcgccgcgcc gtccggtacg ccagtgctgg tgaacggtcg cgaagtgccg
    32701 attgtcgggc gcgtggcgat ggatatgatc tgcgtagact taggtccaca ggcgcaggat
    32761 aaagccgggg ccccggtcat tttatggggc gaaggtttgc ccgtagaacg tatcgctgaa
    32821 atgacgaagg taagtgctta cgaacttatt acgcgcctga cttcaagagt cgcgatgaaa
    32881 tacgtggatt aatattcgtc tccagggagg gacaatgtct tattcagaaa tcgccgcgtg
    32941 tctggcatac gcggtttccg tctggctggc tgccagaaac aatgtgcata catggtggat
    33001 cggcataatt ggcagcatat tgtacggctg ggttttttgg tccgtgcaac tctatgccga
    33061 cgttacgctc cagttattct tcatcgtgac cagcatcact ggctggatcc actggctgaa
    33121 aggtcagggt ggcgacatct tgccggtgcg ccgaacgcaa gccagtcact ttttcctttt
    33181 gttgctctgt gctgtcgtcg tggcaggcgg ttacggcttt ttactccaca cctttaccaa
    33241 tgcttgggca ccctggctgg attcgttgat tttgaccttc agcgttctgg cacaattcat
    33301 gttgatggga agacgtatcg aaaactggta cgtctggtta gcggtgaata ccctggcggt
    33361 gccactgtat atgacgcgcg gtttaaacct gaccgctggc ttatatttcc tgttctggat
    33421 taacgcctgg tatggtttgt atcaatggcg caaagagttg caaacatcat gaagcctttt
    33481 gcaacgggtc tggtggttgg taaattcgcg cctttgcatt gtggtcatga aaagttgatc
    33541 aataccgcac tggcgcaatg tgaagaactg ttcatcatca gttattccgt gccggaaatg
    33601 cctgactgtg aacctgaaaa gcgcctgacg tggctgcaag tgcgcttccc gcaagcgacg
    33661 tttttggtac tgacgccaga gcttgttgcg cgttataact tacctgctat cccgcataac
    33721 aatgctgatg ccgacatcca ccgtcattat gtggcgacgc tatgcttgca gatattacgt
    33781 tgccgcccac acgctgtatt tactgcagaa gactatggcg atggctttgc taacgtgctg
    33841 gcgcggcgtt ttgcccaacc cgttgaacac gttcgaatgg cgcgcccagt tggcgatgaa
    33901 gcgccttcgg gaacgcttat tcgttccgat gttcaccgct atcgctacat gttggcgaat
    33961 gatgtttatt acagtttcgt tcgccgtatc tgtctgcttg gtggcgaatc taccggtaaa
    34021 agcacgcttt caaaagcgct ggcgggcgtg cttgatacgg tgtatgtcgc tgaatttggc
    34081 cgcgactatt gggaagaaaa aaacggtatc ttaactgcgg atgatttgtt gcatatcgcc
    34141 tgcgaacagg tacgccggga acaacaagca gaagctaatc actatcttat atgtgacacg
    34201 tcgccactca cgaccttatt ctatgccctt gatcaatatg gtcatgctcc gcaagaactt
    34261 caccaactgg cggagcggga atactctctg gtagtgcttt gcggcgcaga atttcccttc
    34321 gtgcaggatg gcacccggca aggagaggta tttcgcgcac ggcaacaggt gtggtatgaa
    34381 caggaattgt cgcgcaggaa cattccttat ctttcggttt cggggccact gcaagagcga
    34441 gtggagcaaa ttcttcgcca acttcccgac tgactttgaa catcctctcg atcttcgcct
    34501 tcttccggtt tattgtggtt taaccacctg cccgtaaacc tggagaacca tcgcgtgttt
    34561 caaaaagttg acgcctacgc tggcgacccg attcttacgc ttatggagcg ttttaaagaa
    34621 gaccctcgca gcgacaaagt gaatttaagt atcggtctgt actacaacga agacggaatt
    34681 attccacaac tgaaagccgt ggcggatgcg gaagcgcgcc tgaatgcgca gcctcatggt
    34741 gcttcgcttt atttaccgat ggaagggctt aacagctatc gccatgccat tgcgccgctg
    34801 ctgtttggtg cggaccatcc ggtactgcaa caacagcgcg tagcaaccat tcaaaccctt
    34861 ggcggctcag gggcattgaa agtgggcgca gatttcctga aacgctactt cccggaatca
    34921 agcgtctggg tcagcgatcc tacctgggaa aaccacgtag caatattcgc cggggctgga
    34981 ttcgaagtaa gtacttaccc ctggtatgac gaagcgacta acggcgtgcg ctttaatgac
    35041 ctgttggcga cgctgaaaac attacctgcc cgcagtattg tgttgctgca tccatgttgc
    35101 cacaacccaa cgggtgccga tctcactaat gagcagtggg atgcggtgat tgaaattctc
    35161 aaagcccgtg agcttatccc atttcttgat attgcctatc aaggatttgg tgccggtatg
    35221 gaagaggatg cctacgccat tcgcgccatt gccagcgctg gattacccgc tctggtgagc
    35281 aattcgttct cgaaaatttt ctccctttac ggcgagcgcg tcggcggact ttctgttctg
    35341 tgtgaagatg ccgaagccgc aggccgcgta ctggggcaat tgaaagcaac tgttcgccgc
    35401 aactactcca gcccgccgaa ttttggtgcg caggtggtgg ctgcggtgct gaatgacgag
    35461 gcattgaaag ccagctggct ggtggaagta gaagagatgc gaactcgcat tctggcaatg
    35521 cgtcaggaac tggtgaaggt attgagcaca gagatgccag aacgcaattt cgattatctg
    35581 cttaatcagc gcggcatgtt cagttatacc ggtttaagtg ccgctcaggt tgaccaacta
    35641 cgtgaagaat ttggtgtcta tctcatcgcc agcggtcgca tgtgtgtcgc cgggcttaac
    35701 acgcaaaatg tgcatcgcgt ggcaaaggcg tttgctgcgg tgatgtgatt ttaatcaaca
    35761 ggcggatctc tccgcctgtt cctgactcca gccactccct actattttgt cccttttttc
    35821 gcccactttt acatcatctc ttttctataa gtttttgaaa attagtgttt ttaactgttg
    35881 gtacggattg tgcagtaatg cagctgtcta tgaccatcag gagaaaaaca tggagaacat
    35941 gacctcgccc ggcacgctgc ttagtggaga taacgcgacc tggcttgaag agtactacca
    36001 gacctggttg cgtacgccgg aacaactccc ggaagactgg cggcgttttt ttctttcacc
    36061 tgaactgact gtccaaagcg tatccggtga taacaatatc agcggtgcga ccttaaaaaa
    36121 acaggctgcg gtaatacaac tgatcaatgc ctggcgcacg caggggcatc tgcgcgcaaa
    36181 gcttgatcca cttggtttga atcccccggc tgacgttccc tccctccagc ctggtttttg
    36241 gggattaagc gaagaagact tgttgcagga gtttagcgtc acctttggcg cacacaccac
    36301 gcaaatgcca cttaagcaac tgcttaacct gctggagcaa gcctgggcca gtagccaggc
    36361 ttatgaactt gctcatctgg aaaatcgtga agaaatcaac tggttgttat cacggattga
    36421 gagcagcaat gcgccacaag ctgatgccca aacctgtatt gctcgttttg aaaaactgat
    36481 ggcggcagag acgttagagc gttatctgca tacccgttat gttgggcaga aacggttttc
    36541 tctggaaggg ggcgaaagtg caatcccggc gctcgatacg ctgacaaaac gcctgcgtgc
    36601 gcaaggtgtg gaggaaatgg tcattggcat ggcgcaccgg ggaagactta atgttctggt
    36661 taatctgttg aataaagacc cggcacagct ttttgctgag tttgaaggca agcagacgat
    36721 cggcagtggt tcaggcgatg tgaaatacca catggggtat tccagcaatc tggaaacacc
    36781 tgccggttcg ctgcatgtgg cgctggcgta taacccctca catctggaga tcgtgaatcc
    36841 ggtggtgctg ggccaggtgc gagccaggca ggaacgccgt ggtgaggatg gtcaggcaaa
    36901 agtggtgggc gtacttattc atggggattc cgcgttgggc ggcctcggcg tcaaccaaac
    36961 cacgtttaac ttatcgcaga ctcagggcta cggcactggc ggtacgctac atctggtgat
    37021 taacaatcag attggcttca ccacttcccg cttgcaggat atgcgttctt cacggtactg
    37081 caccgatatt gccaaaatgg tagcggcccc aattatccac gttaatggcg atgacgttga
    37141 cgctgtttgc caggtaatgg aactggcgtg cgaatggcga gacaccttcc ggcgtgacat
    37201 catcatcgat atttgctgct tccgtaagca cggacacaac gaaagcgatg agccacgtct
    37261 tacccagcca caaatgtatc aggcagtgga tgctcaccct ggtacgctcg cccgttatgg
    37321 tgaatctctt gcccgccgcg gcctgttaac tcaggcgcaa caagatgaga tgacggcacg
    37381 ctatcgcgac tggctggata gctgccagaa acgtgaaccg cagcctctca aacctgcaat
    37441 tcactctttt agcgctaact ggtatgggtt gaccaaccct cactggagcg ccccggtatc
    37501 tacggcattg cctcggcaaa agctggcagc gtatggtgaa atcatttcta cactgccacc
    37561 cgatgtggtc gcacatccta ccattaaacg gcaactggcg ttacgccagg atatggccgc
    37621 aggtactcag ccagtagact ggggaatggc tgaaatgctg gcctatgcat cgcttgtgga
    37681 tgcgggtgtt ggtgtgcgtc tttctggtga agactcaggg cgtggcacgt tcagtcatcg
    37741 ccatgcagtg gtacatcatc aaacggaagc ccgtcgttat ctgccgttac agcatatccg
    37801 cgctggtcag gcatctttcg atgtttatga ttcggtgctt aatgaagaag cgctcttagc
    37861 ctttgaatat ggctattcaa cgtctgcgcc tcagcaactg gttatttggg aagcacagtt
    37921 tggtgacttt gccaacggcg ctcaggttgc tattgaccaa tttatctcgt ctggcgaaac
    37981 caagtgggat cgctatagcg gtttaaccat tcttctgccg catggctatg acggacaagg
    38041 gccggagcac tcttccgcac gtcctgagcg ctggctgcaa ctgtgcgcag aaaacaacat
    38101 gcaggtggtg atgcccagtg agtcagcaca aatgttccat ttgttgcgcg gtcaggcatt
    38161 acgcccaatg cgcaaaccgt tagtgataat gatgagtaag cgtttacttc ggtttaaagg
    38221 cgcgatgagc gagctttgtg aattcactga tgacgcttat aaaccagtaa tcaccgatcc
    38281 gcaattgcat cagtcccaga aagttaagcg cgttattttg tgtagcggcc aggtctatta
    38341 cgacgtactg gaagcccgta agcagcgtga gcatgaagac gaggtagcga ttgttcgcct
    38401 ggagcaactc tacccgttcc cggtcgcaga actgaatgat gttctggcga gttggccgaa
    38461 ctgctgcgaa tggatttggt tgcaggaaga gccagaaaac cagggcgcgt ggcggcagat
    38521 tcgtcatgag ctggcggcac tgaaaattaa cactccttac tggcagtatg caggccgccc
    38581 ggcagcggca gcaccagcga cagggtatgg gcgtgtacat aaacaacaaa tcgacgaatt
    38641 tcttgctgct gcgtttgcag atattcagcc atgaaaggcg cacaaaaata ataaggagaa
    38701 cattttatga tcgaaattac tgtccctgta ttaccggaat ctgtcacgga aggcacgctg
    38761 acaacctggt gtaaacaaga aggcgagcac gtaaaacgcg atgatgtgat tgccgagctg
    38821 gaaaccgata aagtcattct ggaaataccg gccccgcacg atggcgtgtt aagcaatatt
    38881 atcgtcagcg aaggtagcac ggtcacgtcc gcgcaactgt tggcgcatct taagccgcaa
    38941 gctgtcatag aagaaaccgt cacaccagtc accgaaaccc tggcgatgcc ttccgcacgg
    39001 ctggaggcac agcgtagtgg tgtcgaactt gctgacgttg cggggagcgg gagaaatgga
    39061 cgtatcctga aagaggatgt gcagcgcgtt actcctgcac cagccattca gcctgaacgg
    39121 gtggcggaga tcgcgccagc taaacccttg acaccgggtg ctcgtcagga acgtcgtgag
    39181 ccgatgtcgc gcttacgtca gcgaattgct gagcgtctgc tggcttccca acaaaataac
    39241 gccattctta ccacattcaa cgaagtaaac atgcagagcg tgatggactt acgcgcccgc
    39301 tggaaagatc gttttgccga gaagcacggc gtgaagctgg gctttatgtc cttctttgtt
    39361 aaggccgtta cccgggcgct ggagcgcttc cctgtagtga atgccagcgt cgatggcaac
    39421 gagattatct ggcgtgatta ttgcgatatc ggcattgcag tgagcagcaa ccgcggcctg
    39481 gtagtgcctg tgttacgcaa tgcgcaatca ctctctttgg tggaaattga acggcaaatt
    39541 gccgaatacg ccacacaggc acgcaatggc aaattgccgc tggaggcgtt acagggcggc
    39601 acgttctcta tcaccaacgg cggtactttt ggttcgatga tgtcaacgcc aatcattaac
    39661 ccgccgcagt cggccattct tgggatgcat gccatcacgc cgcgcccggt cgcagaaaac
    39721 ggtcaggttg ttattcgtcc catgatgtac cttgctctca gctatgacca tcgcattatc
    39781 gatggtcagg aagccgtgca tacattggtg gcaatacgtg agctactgga atcaccggaa
    39841 caactgttgc tggatcttta aggagggcgc cgatgagtac aatttttgat gtggcagtta
    39901 tggggggggg gccaggtggt tacgtagcgg cactgcgggc tgcgcagaat gggctttccg
    39961 tagtatgtat tgatgatggt gttaatgcac agggcgaacc ttcgccgggc ggtacgtgtc
    40021 tcaatgtcgg ctgtattccg tcaaaatcgt tgctgcaatc ttccgaactg tacgcgcaag
    40081 tgcagcatga ggcgagcatt catggcgtta atgttgacgg cgtatctttc aatgcagctg
    40141 cgatgattca acgtaaagac gccatagtaa gccgcctgac aatgggcatc agcttgctgt
    40201 ttaagaaaaa taaagtgaag cacctgtgcg gcctcgcaac gttagaatgt gctcaggatg
    40261 agatttggca attacgtgta aacgatcaac accttcacgc ccgtaatgta gtgattgcaa
    40321 caggttctca gccgcgtcag ctgcctggcg ttaccattga taatcaacag atcctcgaca
    40381 atcgtggcgc cctggcattg agcgaagtgc cgccgcgtct cggtgtgatc ggcgctggcg
    40441 tgattggcct tgaactgggg tctgtgtgga atcgggttgg ttcagacgtc acgttgctgg
    40501 agatggcgcc aacgttcttg cctgcactgg aagctcgcct gtcgaacgaa gtccgtaaag
    40561 caatgattgc cagtgggatg aaaatgcaac tggcagtaga gattgaggct attgaacaac
    40621 gggatgacgg tgtgcatgtg cgctggcggc agggtgagaa gcgagaagaa agccgcttcg
    40681 ataaattaat tcttgccatt ggacgtgtac cgcgactttc tggtgtcgat ctcgtgcaac
    40741 tcggtcttga agccgataac cgtggcggta ttgccgtcga taacttatgc cgtaccggga
    40801 aagccgggct gtgggcaatt ggtgatgtcg tgcgagggcc gatgctggcg cataaagcga
    40861 tggctgaagg tgtagtggtg gctgatcaaa ttgccggact ggtggtagag ccgattaatt
    40921 ttgcgctgat cccttcggtg atttacaccc aaccggaagt tgcgtgggta ggggaaaacg
    40981 aggcctcact gaaagccgct ggcagagtct ttaacaaagg aaattctctg tttgccggta
    41041 acggtcgcgc cttagcatta ggccaggaag gtgggcgttg cacgttatac agtgataaac
    41101 acactgaccg cgtacttggc ggagcgattg ttggcccgca ggcatcggag ctgattaatg
    41161 aaattgcgct ggcgatgact tttagcgcct ctggcgagga tattgcctgt gcgatccatg
    41221 cgcatccgac attaagcgaa gtcatccacg aagcagcaat ggccttgaat aataaagcat
    41281 tacacggata acacacacca ctgagggaat atatgaattt acatgagtat caggccaaat
    41341 cgttactggc tggcatgggg atgccgtgtc caaaagagat agctatccaa cagatttcgc
    41401 aactggcaga tgcctggcaa catattgctt gcccgagtaa aggcgcggta cttaaagcgc
    41461 aggtacatgc tggtgggcgc ggtaaagctg gcggcgtaaa agtgcttaag cagctgccgg
    41521 aagcacaggc ttttgtacag caaatgttgg gatcgcaact ggtgacttat cagaccgggc
    41581 cagaaggaca gtatgtcagc agtattttac tgtgcgaaaa catttatccg gtacgccagg
    41641 aactctattt tggcatggtg gtggatcgtg aaagccagcg ggtcacgttt attgtcagcc
    41701 cggaaggtgg tgtggaaatt gaaaaagtcg cccatgagac accggagaaa atcagcagtg
    41761 tcagcattga tccactgaca ggcgtgcagc cttgccatat ccgcgaaatg tttgctgttc
    41821 tgcaactgga acacggacta tttgctgcct ttagtcgcct ggttaatcag gcgtggaaag
    41881 cctttaacga actggatttt gccctgctgg aaattaaccc tctggtgttg cgggaaacgg
    41941 gcgaatttat gtgtgcagac gcgaaagtat cgctggatga taacgcgctg tatcgtcacc
    42001 cggaactcca ggtgctacgt gatgaaaccc aggaagatcc acgcgaaagc caggcagcga
    42061 agcttgatct taattatgtc tcgctggacg gaaatatcgg ctgcatggtc aatggtgcag
    42121 ggctggctat ggcaaccatg gacatcatca agctatacgg tgaacaaccc gcaaacttcc
    42181 tggatgttgg cggtggcgca acccaggagc gcgtcagtga agcctttcgg ctgattgttt
    42241 ccgacagcaa agtgaaagcc attctggtta atatttttgg tgggattgtc cgttgcgaca
    42301 tgatcgccag agccattatt catgctctga atgaggccag catcaccctg cctgtcgtgg
    42361 tgcgtctctc cggtaataac gccgccgaag ggcagcggtt actggcagaa agtggtctga
    42421 cggtggaagc cgtcgattct ctggatgatg ccgcgaaacg catcattgcg ttactgaatt
    42481 aacaggaggt taagatgagc gttttgatca acaaacacac gcgggtactg gtgcagggca
    42541 ttaccggcaa aaacggcaca tttcatacag agcaggctat tgcgtatggc acgcagattg
    42601 tgggtggagt gacgcctggt aagggcgggc aacggcatct ggatcgcccg gtttttgatt
    42661 caatgaaaga tgcgatgcga caaactgagg ccgatgccag cgtgatttat gtgcctgcac
    42721 cgtttgtgct ggattctgtt gttgaggcta ttgaagctgg cgtcaaattg gttgtggtga
    42781 tcactgaagg tgtaccgaca ctggatatgg tgaaagttcg tcggctgctt gactgccacc
    42841 cggatgtgcg gctgattggg ccaaactgcc caggagttat cacgccgggt gagtgcaaaa
    42901 tcgggattat gcctggtgaa atccaccgcc cagggcgtat tgggattgtt tcccgctccg
    42961 gtacattaac ctacgaagct gtagcgcaaa ccacggcgct gggacttggg caatccacct
    43021 gtattggtat tggcggcgat ccggttccgg ggactaactt tattgatgcg ctgcgtttat
    43081 tcgaaaatga tccgcaaaca gacgcggtaa ttatgatcgg tgaaattggt ggtaatgcgg
    43141 aagaacgggc cgcggaattt attcgccatg atatgaataa accggtcgta ggttatattg
    43201 ccggtgttac tgcaccaaaa ggaaaacgca tgggacacgc cggagccatt atttctggcg
    43261 gtagcggcag tgcagaggat aaattccgtg cgttcgataa agcaggcatg gcatggacaa
    43321 gaaacccggc gttgcttggc gaaacgttat ggaatgttat taagtaatcc ataaatgaat
    43381 atcaagggag catatcgctc ccttttttta tctgtgtggt aagagaatca ccttttattt
    43441 actgtgcgga aaaccgcgca caaaataaaa agaataaata ataaaatcat taaataacaa
    43501 agcattatta aaaaattaaa gatggcatag ctcctgcaat agccaggcag gttttttatt
    43561 tttcacaatg aggtgaatat gtcatcaata tcacatggcg caccacaaaa gcgtcgaatt
    43621 atccctaatc ccggcctgtg gttggcgatc attgccggaa ttattattac tcttctgcct
    43681 ctgggcgata ccttgcccgt cgccggacaa aatatgatcg ccattctggt gtttgccatt
    43741 attgtctgga tcagcgaagc gatggattac accgccagtg caattgttat ttccgcactg
    43801 attatcttta tggttggttt tgccccggat atgaatcacc cggacacgat ccttggcacc
    43861 gcgaaagcgc tgaaaatgac tctgtcgggt ttttctaatt ctgctctggc gctggtggca
    43921 gcagcaatgt ttatcgcggc ggcaatgacc attaccgggc tggataaacg tattgcgctg
    43981 tttactatgt cgaaaatagg tgccagtagc cgcagcatta ttatcggtgc cattgtggtg
    44041 accatcgtcc tgagcctggt cgtacccagt gccactgccc gtacggcttg cgtggtgccg
    44101 attatgatgg gggtcattgc ggcatttaag gtggataaac attcacgttt agcggcgtca
    44161 atgatgattg ttatcgccca ggcgaccagt atctggaacg ttggtattca aacttcggcg
    44221 gcgcaaaacc tgctttctat cggttttatc aataaaactt tcggcgccgg gcattctgtc
    44281 agctggcttg actggctgtt agcgggcgcg ccctggagcc tcaccatgtc ggtgatcctc
    44341 tatttcctcg cacgtaaatt gttgccgcca gaaacggaag ctgttgaagg ggggagcgag
    44401 gccattaaaa aagcactcgc agagttaggg ccaaccaccg gtaaagaaaa acgcttgatc
    44461 ggtatttcgc tgttgttact gctcttttgg tctactggcg gaaaattaca cagtattgat
    44521 accacctctg tcacccttgc cggactggcg attatgttgc tgccaggcat cggtgtaatg
    44581 agctggaaag aggtcgaaaa acgtgtgcag tggggcacgt tgctgatgtt tggtattggt
    44641 atcagcctgg ggtccacgtt gcttgataca caggcagctt catggatggc gaactatgtg
    44701 gtgaaaggtt ttggtcttga cggtttacca tcacttgcca tcttcgcgat tctcgccgct
    44761 ttcttgataa tcattcacct ggggtttgcc agtgccactg cgctaactgc cgcattgctg
    44821 cccatcctga ttagcctgtt aagcagtctg ccgccagaac tcggcgttaa cccggtcggc
    44881 atgaccattc tgctcgcctt cagtgtcagt tttggcttca tcctgccgat taacgcgcca
    44941 caaaatatgg tgtgtatggg tacggatacc ttcacgccac gccagtttac ccgtgtggga
    45001 ctgtacctga cggtaatcgg ctacctgttg ttgctactgt ttgccgccac ctggtggaaa
    45061 attttaggtc taatgtgagg aaaccaacga tgttattaca aattgctgtt gaaaaagtgc
    45121 gttggttagc tgccgggctg ctggaactta atggctgtga tgccgatatc gctcaggatg
    45181 tggcggaaca tatgattgaa gcggaacgtt atggctttgc cagccacggc gtcacgctgc
    45241 taccgaaata tctggaaaat atcgctcgtg gcgatgtaac cgcgaatgcc cgcccggaat
    45301 gcttaacaag tgaaggtaat ttacaacgtt ttcatgccca caatggcttt ggtcaacacg
    45361 ccggaaaagt ggcggtaaat gctgccatcg aacaagcaaa acaccgcggt ttttgcctgt
    45421 taacgttgtg ccatgcgcat catttagggc gtatggggca ttacgggcaa atggttgctg
    45481 atcaaggcct ggcgatgctg gccatgagta acgtcaccgg gcgtcctgca ctggtcgccc
    45541 cgtggggcgg tgcggaaccg cgtatgacca ctaacccttt ctgctttgcc tggccgttca
    45601 gtgatgggcg tccgccgatt ctggttgatt ttgccaccag tagcatggcg ctaaataaag
    45661 cacgggtaat gtcgtccacc gggaagcagg ccgcgccggg acaattgatt gatgcacagg
    45721 ggaatccaag caatgatcct ggggtgttat tcagcaatcc tccaggggcg ttactgccat
    45781 ttggtgagca taaaggattt ggcctggcac tgatgattga aatgatggcg gggatcttat
    45841 ctggtggtga cacgatcgcc gaagatcatc agacggatgg atctgcgcat aatcatcttt
    45901 ttgcgttgat tatcgatcct gatcaattca ccgatctggc aggggagaaa gggcagttct
    45961 ttgcggatta tctgttagct acgcagcctc aacctggcgg caatccggtg atttaccccg
    46021 gtatgccaga agctgccaac cgggaacgca atgcgaagat gattacgatc ccggtatcat
    46081 tctggtcctg ggtagttgaa cattacgccc gcaaaggtat cgatttgggg ttacatcctc
    46141 aggaatcagc attagctgga tgattctggt cgataaactt gcggttgaat ctgatgtttg
    46201 ttgagtttgt catataaagt cttgcgcggc agctgtaata gccgcgctgt caaactgacc
    46261 tggccttccg tttgtcgcag catatcttcg attaactttt tctcgaaagc atcaatgcag
    46321 gcggtaagac cactctcttc ttgctgagtc attggcactg gctggtggtg ggtcagtaaa
    46381 ccgagaacaa aacgctctgc attgtgttta agttcacgta cattgccagg ccaactttgt
    46441 gactgcaacc aggccagtaa catgggagaa atatcaggca gaggacgatt atatttttgt
    46501 gccgcctgac tggcgaacca gtaaaacagt tcagggatat cttctcgtcg ctggcgtaga
    46561 ggtggaatat tcaggctaac cacattcaga cgataataga gatcgagacg aaacagatgt
    46621 tcttcgctgc gacgcagtaa atcttccttg gtcgctgcaa taacacggca attcaccgga
    46681 attaattgat tggcacctaa tcgttcaatg gttctttcct gcaatacacg tagcagtttt
    46741 acctgcattc ctgatggcat tccctctatt tcatcgagaa ataacgtacc accatttgca
    46801 tgttcaattt ttccaatacg ctttttaacc gcaccggtaa atgcgcccgc ctcatgacca
    46861 aatatttcgc tttcaaacaa cgtttctggt aagcctgcac agtttaaggc aacaaactgc
    46921 ccctgacggc gggtactcca gtgatgcagc attctggcga cgacttcttt accacaacct
    46981 gtttcaccat aaataaggac atcggcaccg gtatctgcga cattaaggat cattttacgc
    47041 agttgctgca tctgcggact acgaccaatc agcaccgggc cattttcctg ttgcagattc
    47101 gccagtaatt gctgattttc taaaaccaat cgccgttttt ctaccgctct ggcaataata
    47161 ctcaataatt tatctgagct acttggtttc tcaatgaaat cgaatgcacc attacgcatg
    47221 gcatccaccg ccatttccac gtcaccgtgt ccggtaatta aaataaccgg aattttgcta
    47281 tccatttcgt tgatgtaact gaggagctct agtcctgata ttccaggtaa cctgacatcg
    47341 gtcagtataa taatgttggc ctgactttga ataaggggaa tactgtgttc tgcatcgcaa
    47401 caggaaataa cgcgataacc agccagcgtg agcgtttgtt cacaggcaaa acgtacatcg
    47461 tcactatcct caatataaac tatagtaatt tcactgaatt tattattcat gtttttctcc
    47521 ccattcaggc caggttaatg tcattactgc gccgccttca ggatgattac tggcgtggag
    47581 tgcgccgttc gaatttcgga caatttcgct gactattgcc agtcccaacc ccattcctct
    47641 acgtttagtg gtaaaaaatg gttcaaatat gcgatcgaca acttcaggtg caaaaccgcc
    47701 accgctgtct ttaatggtaa taataacttt gttatttgcc tggcgaatgg cgatatccag
    47761 tcgtttgtaa gagctacctt ccatagaatc taaggcgttg ctaattaaat tactgaatat
    47821 ctgttcaaga ccgagctcat cgcaattgac aaataacggc atggatgggg cttttattcg
    47881 tcgctcaatg ttatttttct ccatgctgtg attaagtaac gccacggcgc tatacatcac
    47941 tttgatgaca tcggcagaac ctttaggtac gcgatggcgc gaggcaaatg ctttaagttc
    48001 ggaaatgagc tgcgtcatcc gctcaatcac ggaaataata tgtttcagat tttgttcaac
    48061 ctgcggatac atctcttttt tcagcataat gcgcgcgtta tctgtaagtg cgtgaatggc
    48121 ggctaatggt tgattttgtt catgggcaat ttcggttgcc atctgcccaa gcgccgccag
    48181 tttgctggac tctgccagtt cactgcgcgt aatttgtaat acttgctcag cctgactgcg
    48241 ttctttcatc tcctggagta atttttgatt gatcaattca agggcagatg tacgctcttt
    48301 aacctgtttt tctaatgttt cattcgccag tgttaataat tgttgtgtat gagatcgcat
    48361 tctccagtat ttaataaata acaaaaacag cagataaata attagtgt
//
LOCUS       DAHHTG010000019        47766 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.019, whole genome shotgun sequence.
ACCESSION   DAHHTG010000019 DAHHTG010000000
VERSION     DAHHTG010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 47766)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47766)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47766
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.019"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            149..781
                     /gene="comR"
                     /locus_tag="NBI74_001092"
     CDS             149..781
                     /gene="comR"
                     /locus_tag="NBI74_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family copper-responsive transcriptional
                     repressor ComR"
                     /protein_id="HCD1931459.1"
                     /translation="MATDSTQCVKKSRGRPKVFDRDAALDKAMKLFWQHGYEATSLAD
                     LVEATGAKAPTLYAEFTNKEGLFRAVLDHYIDRFAAKHEAQLFCEEKSVESALADYFA
                     AIANCFTSKDTPAGCFMINNCTTLSPDSGDIANTLKSRHAMQERTLQQFLCQRQARGE
                     IPTHCDVTHLAEFLNCIIQGMSISAREGASLEKLMQIARTTLRLWPELLK"
     gene            complement(843..1382)
                     /locus_tag="NBI74_001093"
     CDS             complement(843..1382)
                     /locus_tag="NBI74_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HCD1931460.1"
                     /translation="MNKSMLAGIGIGVAAALGVAAVASLNVFERGPQYAQVVSATPIK
                     ETVKTPRQECRNVTVTHRRPVQDENRITGSVLGAVAGGVIGHQFGGGRGKDVATVVGA
                     LGGGYAGNQIQGSLQESDTYTTTQQRCKTVYDKSEKMLGYDVTYKIGDQQGKIRMDRD
                     PGTQIPLDSNGQLILNNKV"
     gene            complement(1609..2913)
                     /gene="ndh"
                     /locus_tag="NBI74_001094"
     CDS             complement(1609..2913)
                     /gene="ndh"
                     /locus_tag="NBI74_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017384716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone dehydrogenase"
                     /protein_id="HCD1931461.1"
                     /translation="MTTPLKKIVIVGGGAGGLEMATQLGHKLGRKKKAKITLVDRNHS
                     HLWKPLLHEVATGSLDEGVDALSYLAHARNHGFQFQLGSVIDIDREAKTITIAELRDE
                     KGELLVPERKIAYDTLVMALGSTSNDFNTPGVKENCIFLDNPHQARRFHQEMLNLFLK
                     YSANLGANGKVNIAIVGGGATGVELSAELHNAVKQLHSYGYKGLTNEALNVTLVEAGE
                     RILPALPPRISAAAHSELTKLGVRVLTQTMVTSADEGGLHTKDGEYIEADLMVWAAGI
                     KAPDFLKDIGGLETNRINQLVVEPTLQTTRDPDIYAIGDCASCPRPEGGFVPPRAQAA
                     HQMATCAMNNILAQMNGKPLKNYQYKDHGSLVSLSNFSTVGSLMGNLTRGSMMIEGRI
                     ARFVYISLYRMHQIALHGYFKTGLMMLVGSINRVIRPRLKLH"
     gene            complement(3312..3854)
                     /locus_tag="NBI74_001095"
     CDS             complement(3312..3854)
                     /locus_tag="NBI74_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCD1931462.1"
                     /translation="MIIYLHGFDSNSPGNHEKVLQLQFIDPDVRLISYSTRHPKHDMQ
                     HLLKEVDKMLQLNVDERPLICGVGLGGYWAERIGFLCDIRQVIFNPNLFPYENMEGKI
                     DRPEEYADIATKCVTNFREKNRDRCLVILSRNDEALNSQRTSEELHHYYEIVWDEEQT
                     HKFKNISPHLQRIKAFKTLG"
     gene            complement(3877..4902)
                     /gene="nagZ"
                     /locus_tag="NBI74_001096"
     CDS             complement(3877..4902)
                     /gene="nagZ"
                     /locus_tag="NBI74_001096"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HCD1931463.1"
                     /translation="MGPVMLDVEGYELDAEEREILAHPLVGGLILFTRNYHDPAQLRE
                     LVRQIRAASRNHLVVAVDQEGGRVQRFREGFTRLPAAQSFAALLGMEEGGKLAQEAGW
                     LMASEMIAMDIDISFAPVLDVGHISAAIGERSYHADPQKALAIASRFIDGMHEAGMKT
                     TGKHFPGHGAVTADSHKETPCDPRPQAEIRAKDMSVFSSLIRENKLDAIMPAHVIYSD
                     VDPRPASGSPYWLKTVLRQELGFDGVIFSDDLSMEGAAIMGSYAERGQASLDAGCDMI
                     LVCNNRKGAVSVLDNLSPIKAERVTRLYHKGSFSRQELMDSARWKAISARLNQLHERW
                     QEEKAGH"
     gene            complement(4913..5737)
                     /gene="thiK"
                     /locus_tag="NBI74_001097"
     CDS             complement(4913..5737)
                     /gene="thiK"
                     /locus_tag="NBI74_001097"
                     /EC_number="2.7.1.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine kinase"
                     /protein_id="HCD1931464.1"
                     /translation="MPFRSNNPLTRDELLSRFFPQFHPVTTFNSGLSGGSFLIEHQGQ
                     RFVVRQPHDPDAPQSAFLRQYRALSQLPACIAPKPHLYLRDWMVVDYLPGEVKTYLPD
                     TNELAGLLYYLHQQPRFGWRITLLPLLELYWQQSDPARRTVGWLRMLKRLRKAREPRL
                     LRLSPLHMDVHAGNLVHSASGLKLIDWEYAGDGDIALELAAVWVENTDQHRQLVNDYA
                     TRAKIYPAQLWRQVRRWFPWLLMLKAGWFEYRWRQTGDQQFIRLADDTWRQLLIKQ"
     gene            complement(5718..6359)
                     /gene="lpoB"
                     /locus_tag="NBI74_001098"
     CDS             complement(5718..6359)
                     /gene="lpoB"
                     /locus_tag="NBI74_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoB"
                     /protein_id="HCD1931465.1"
                     /translation="MTKMSRYALITALAMFLAGCVGQREPAPVEEVKPAPEQPTEPQQ
                     PVPTVPSVPTIPQQPGPIEHEDQTAPPAPHIRHYDWNGAMQPMVSKMLGADGVTAGSV
                     LLVDSVNNRTNGSLNAAEATETLRNALANNGKFTLVSAQQLSMAKQQLGLSPQDSLGT
                     RSKAIGIARNVGAHYVLYSSASGNVNAPTLQMQLMLVQTGEIIWSGKGAVSQQ"
     gene            complement(6373..6750)
                     /locus_tag="NBI74_001099"
     CDS             complement(6373..6750)
                     /locus_tag="NBI74_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcfL family protein"
                     /protein_id="HCD1931466.1"
                     /translation="MRKGCFGLVSLALLVLVGCRSHPEIPVNDEQSLVMESSLLAAGI
                     SAEKPVLSTSDIQPSASSTLYNERQEPVTVHYRFYWYDARGLEMHPLERPRSVTIPAH
                     SAVTLYGSANFLGAHKVRLYLYL"
     gene            complement(6753..7112)
                     /gene="hinT"
                     /locus_tag="NBI74_001100"
     CDS             complement(6753..7112)
                     /gene="hinT"
                     /locus_tag="NBI74_001100"
                     /EC_number="3.9.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415621.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside phosphoramidase"
                     /protein_id="HCD1931467.1"
                     /translation="MAEETIFSKIIRREIPSDIVYQDDLVTAFRDISPQAPTHILIIP
                     NILIPTVNDVSAEHEQALGRMITVAAKIAEQEGIAEDGYRLIMNTNRHGGQEVYHIHM
                     HLLGGRPLGPMLAHKGL"
     gene            7446..9635
                     /gene="fhuE"
                     /locus_tag="NBI74_001101"
     CDS             7446..9635
                     /gene="fhuE"
                     /locus_tag="NBI74_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric-rhodotorulic acid/ferric-coprogen
                     receptor FhuE"
                     /protein_id="HCD1931468.1"
                     /translation="MLSTQFNRDNQHQAIIKPSLLAGCIALALLPSAAFAAPATEETV
                     IVEGSATAPDDGENDYNVTSTSAGTKMQMTQRDIPQSVTIVSQQRMEDQQLQTLGEVM
                     ENTLGISKSQADSDRALYYSRGFQIDNYMVDGIPTYFESRWNLGDALSDMALFERVEV
                     VRGATGLMTGTGNPSAAINMVRKHATSREFKGDVSAEYGSWNKERYVADLQSPLTEDG
                     KIRARIVGGYQNNDSWLDRYNSEKTFFSGIVDADLGDLTMLSAGYEYQRIDVNSPTWG
                     GLPRWNTDGSSNSYDRARSTAPDWAYNDKEINKVFMTLKQRFADTWQATLNATHSEVE
                     FDSKMMYVDAYVNKADGMLVGPYSNYGPGFDYVGGTGWNSGKRKVDALDLFADGSYEL
                     FGRQHNLMFGGSYSKQNNRYFSSWANIFPDEIGSFYNFNGNFPQTDRSPQSLAQDDTT
                     HMKSLYAATRVTLADPLHLILGARYTKWRVDTLTYSMEKNHTTPYAGLVFDINDNWST
                     YASYTSIFQPQNDRDSSGKYLAPITGNNYELGLKSDWMNSRLTTTLAIFRIEQDNVAQ
                     STGTPIPGSNGETAYKAVDGTVSKGVEFELNGAITDNWQLTFGATRYIAEDNEGNAVN
                     PNLPRTTVKMFTSYRLPVMPELTVGGGVNWQNRVYTDTVTPYGTFRAEQGSYALVDLF
                     TRYQVTKNFSLQGNVNNLFDKTYDTNVEGSIVYGAPRNLSITGMYQF"
     gene            complement(9695..11128)
                     /gene="ptsG"
                     /locus_tag="NBI74_001102"
     CDS             complement(9695..11128)
                     /gene="ptsG"
                     /locus_tag="NBI74_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000475716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIBC"
                     /protein_id="HCD1931469.1"
                     /translation="MFKNAFANLQKVGKSLMLPVSVLPIAGILLGVGSANFSWLPAVV
                     SHVMAEAGGSVFANMPLIFAIGVALGFTNNDGVSALAAVVAYGIMVKTMAVVAPLVLH
                     LPAEEIASKHLADTGVLGGIISGAIAAYMFNRFYRIKLPEYLGFFAGKRFVPIISGLT
                     AIFTGVVLSFIWPPIGSAIQTFSQWAAYQNPVVAFGIYGFIERCLVPFGLHHIWNVPF
                     QMQIGEYTNAAGQVFHGDIPRYMAGDPTAGKLSGGFLFKMYGLPAAAIAIWHSAKPEN
                     RAKVGGIMISAALTSFLTGITEPIEFSFMFVAPILYIIHAILAGLAFPICILLGMRDG
                     TSFSHGLIDFIVLSGNSSKLWLFPIVGIGYAIVYYTIFRVLIKALDLKTPGREDATED
                     AKATGTSEMAPALVAAFGGKENITNLDACITRLRVSVADVSKVDQAGLKKLGAAGVVV
                     AGSGVQAIFGTKSDNLKTEMDEYIRNH"
     gene            complement(11423..12220)
                     /locus_tag="NBI74_001103"
     CDS             complement(11423..12220)
                     /locus_tag="NBI74_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HCD1931470.1"
                     /translation="MFLVDSHCHLDGLDYESLHKDVDDVLAKAAARDVKFCLAVATTL
                     PGYLHMRDLVGERDNVVFSCGVHPLNQNDPYDVEDLRRLAAEEGVVALGETGLDYYYT
                     PETKVRQQESFIHHIQIGRELNKPVIVHTRDARADTLAILREEKVTDCGGVLHCFTED
                     RETAGKLLDLGFYISFSGIVTFRNAEQLRDAARYVPLDRLLVETDSPYLAPVPHRGKE
                     NQPAMVRDVAEYMAVLKGVAVEELAQVTTDNFARLFHIDASRLQSIR"
     gene            complement(12231..13235)
                     /gene="holB"
                     /locus_tag="NBI74_001104"
     CDS             complement(12231..13235)
                     /gene="holB"
                     /locus_tag="NBI74_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HCD1931471.1"
                     /translation="MRWYPWLRPDFEKLVASYQAGRGHHALLIQALPGMGDDALIYAL
                     SRYLLCQQPQGHKSCGHCRGCQLMQAGTHPDYYTLAPEKGKNALGIDAVREVTEKLNE
                     HARLGGAKVVWVTDAALLTDAAANALLKTLEEPPAETWFFLATREPERLLATLRSRCR
                     LHYLAPPPEQYAVTWLSREVTMSQDALLAALRLSAGSPGAALALFQGDNWQARETLCQ
                     ALAYGVQSGDWYSLLAALNNEQAPARLHWLATLLMDALKRHYGAAHVTNVDVPGLVVE
                     LANHLSPSRLQAILGDVCHIREQLMSVTGINRELLITDLLLRIEHYLQPGVVLPVPHL
                     "
     gene            complement(13232..13873)
                     /locus_tag="NBI74_001105"
     CDS             complement(13232..13873)
                     /locus_tag="NBI74_001105"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HCD1931472.1"
                     /translation="MRSKYIVIEGLEGAGKTTARNVVVETLEQLGIRDMVFTREPGGT
                     QLAEKLRSLVLDIKSVGDEVITDKAEVLMFYAARVQLVETVIKPALANGTWVIGDRHD
                     LSTQAYQGGGRGIDQHMLATLRDAVLGDFRPDLTLYLDVTPEVGLKRARARGELDRIE
                     QESFDFFNRTRARYLELAAQDKSIHTIDATQPLEAVMDAIRTTVTNWVKELDA"
     gene            complement(13863..14885)
                     /gene="yceG"
                     /locus_tag="NBI74_001106"
     CDS             complement(13863..14885)
                     /gene="yceG"
                     /locus_tag="NBI74_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YceG"
                     /protein_id="HCD1931473.1"
                     /translation="MKKVLLIILLLLVVLGIAAGVGVWKVRHLADSKLLIKEETIFTL
                     KPGTGRLALGEQLYADKIINRPRVFQWLLRIEPDLSHFKAGTYRFTPQMTVREMLKLL
                     ESGKEAQFPLRLVEGMRLSDYLKQLREAPYIKHTLSDDKYATVAQALELENPEWIEGW
                     FWPDTWMYTANTTDVALLKRAHKKMVKAVDSAWEGRADGLPYKDKNQLVTMASIIEKE
                     TAVASERDQVASVFINRLRIGMRLQTDPTVIYGMGERYNGKLSRADLETPTAYNTYTI
                     TGLPPGAIATPGADSLKAAAHPAKTPYLYFVADGKGGHTFNTNLASHNKSVQDYLKVL
                     KEKNAQ"
     gene            complement(14888..15697)
                     /gene="pabC"
                     /locus_tag="NBI74_001107"
     CDS             complement(14888..15697)
                     /gene="pabC"
                     /locus_tag="NBI74_001107"
                     /EC_number="4.1.3.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate lyase"
                     /protein_id="HCD1931474.1"
                     /translation="MFLINGHKQESLAVSDRATQFGDGCFTTARVIDGKVSLLSAHIQ
                     RLQDACQRLMISCDFWPQLEQEMKTLAAEQQNGVLKVVISRGSGGRGYSTLNSGPATR
                     ILSVTAYPAHYDRLRNEGMTLALSPVRLGRNPHLAGIKHLNRLEQVLIRSHLEQTNAD
                     EALVLDSEGWVTECCAANLFWRKGNVVYTPRLDQAGVNGIMRQFCIRLLAQSSYQLVE
                     VQASLEEALQADEMVICNALMPVMPVRACGDVSFSSATLYEYLAPLCERPN"
     gene            complement(15817..17058)
                     /gene="fabF"
                     /locus_tag="NBI74_001108"
     CDS             complement(15817..17058)
                     /gene="fabF"
                     /locus_tag="NBI74_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HCD1931475.1"
                     /translation="MSKRRVVVTGLGMLSPVGNTVESTWKALLAGQSGISLIDHFDTS
                     AYATKFAGLVKDFNCEDIISRKEQRKMDAFIQYGIVAGVQAMQDSGLEITEENATRIG
                     AAIGSGIGGLGLIEENHTSLMNGGPRKISPFFVPSTIVNMVAGHLTIMYGLRGPSISI
                     ATACTSGVHNIGHAARIIAYGDADVMVAGGAEKASTPLGVGGFGAARALSTRNDNPQA
                     ASRPWDKERDGFVLGDGAGMLVLEEYEHAKKRGAKIYAELVGFGMSSDAYHMTSPPEN
                     GAGAALAMANALRDAGIEASQIGYVNAHGTSTPAGDKAEAQAVKTIFGEAASRVLVSS
                     TKSMTGHLLGAAGAVESIYSILALRDQAVPPTINLDNPDEGCDLDFVPHEARQVSGME
                     YTLCNSFGFGGTNGSLIFKKI"
     gene            complement(17146..17382)
                     /gene="acpP"
                     /locus_tag="NBI74_001109"
     CDS             complement(17146..17382)
                     /gene="acpP"
                     /locus_tag="NBI74_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HCD1931476.1"
                     /translation="MSTIEERVKKIIGEQLGVKQEEVTNNASFVEDLGADSLDTVELV
                     MALEEEFDTEIPDEEAEKITTVQAAIDYINGHQA"
     gene            complement(17593..18327)
                     /gene="fabG"
                     /locus_tag="NBI74_001110"
     CDS             complement(17593..18327)
                     /gene="fabG"
                     /locus_tag="NBI74_001110"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HCD1931477.1"
                     /translation="MNFEGKIALVTGASRGIGRAIAETLAARGAKVIGTATSENGAQA
                     ISDYLGANGKGLMLNVTDPASIESVLEKIRAEFGEVDILVNNAGITRDNLLMRMKDEE
                     WNDIIETNLSSVFRLSKAVMRAMMKKRHGRIITIGSVVGTMGNGGQANYAAAKAGLIG
                     FSKSLAREVASRGITVNVVAPGFIETDMTRALSDDQRAGILAQVPAGRLGGAQEIANA
                     VAFLASDEAAYITGETLHVNGGMYMV"
     gene            complement(18340..19269)
                     /gene="fabD"
                     /locus_tag="NBI74_001111"
     CDS             complement(18340..19269)
                     /gene="fabD"
                     /locus_tag="NBI74_001111"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HCD1931478.1"
                     /translation="MTQFAFVFPGQGSQTVGMLADMAASYPIVEETFAEASAALGYDL
                     WALTQQGPAEELNKTWQTQPALLTASVALYRVWQQQGGKAPAMMAGHSLGEYSALVCA
                     GVIDFADAVRLVEMRGKFMQEAVPEGTGAMAAIIGLDDASIAKACEEAAEGQVVSPVN
                     FNSPGQVVIAGHKEAVERAGAACKAAGAKRALPLPVSVPSHCALMKPAADKLAVELAK
                     ITFNAPTVPVVNNVDVKCETNGDAIRDALVRQLYNPVQWTKSVEYMAAQGVEHLYEVG
                     PGKVLTGLTKRIVDTLTASALNEPSAMAAALEL"
     gene            complement(19285..20238)
                     /gene="fabH"
                     /locus_tag="NBI74_001112"
     CDS             complement(19285..20238)
                     /gene="fabH"
                     /locus_tag="NBI74_001112"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HCD1931479.1"
                     /translation="MYTKIIGTGSYLPEQVRTNADLEKMVDTSDEWIVTRTGIRERHI
                     AAPNETVSTMGFEAATRAIEMAGIEKDQIGLIVVATTSATHAFPSAACQIQSMLGIKG
                     CPAFDVAAACAGFTYALSVADQYVKSGAVKYALVVGSDVLARTCDPTDRGTIIIFGDG
                     AGAAVLAASEEPGIISTHLHADGSYGELLTLPNADRVNPENSIHLTMAGNEVFKVAVT
                     ELAHIVDETLTANNLDRSQLDWLVPHQANLRIISATAKKLGMSMDNVVVTLDRHGNTS
                     AASVPCALDEAVRDGRIKPGQLVLLEAFGGGFTWGSALVRF"
     gene            complement(20306..21376)
                     /gene="plsX"
                     /locus_tag="NBI74_001113"
     CDS             complement(20306..21376)
                     /gene="plsX"
                     /locus_tag="NBI74_001113"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HCD1931480.1"
                     /translation="MTRLTLALDVMGGDFGPSVTVPAALQALNSNSQLTLLLVGNPDA
                     ITPLLAKADFEQRSRLQIIPAQSVIASDARPSQAIRASRGSSMRVALELVKEGRAQAC
                     VSAGNTGALMGLAKLLLKPLEGIERPALVTVLPHQQKGKTVVLDLGANVDCDSTMLVQ
                     FAIMGSVLAEEVVEIPNPRVALLNIGEEEVKGLDSIRDASAVLKTIPSINYIGYLEAN
                     ELLTGKTDVLVCDGFTGNVTLKTMEGVVRMFLSLLKSQGEGKKRSWWLLLLKRWLQKS
                     LTRRFSHLNPDQYNGACLLGLRGTVIKSHGAANQRAFAVAIEQAVQAVQRQVPQRIAA
                     RLESVYPAGFELLDGGKSGTLR"
     gene            complement(21457..21630)
                     /gene="rpmF"
                     /locus_tag="NBI74_001114"
     CDS             complement(21457..21630)
                     /gene="rpmF"
                     /locus_tag="NBI74_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HCD1931481.1"
                     /translation="MAVQQNKPTRSKRGMRRSHDALTAVTSLSVDKTSGEKHLRHHIT
                     ADGYYRGRKVIAK"
     gene            complement(21682..22203)
                     /gene="yceD"
                     /locus_tag="NBI74_001115"
     CDS             complement(21682..22203)
                     /gene="yceD"
                     /locus_tag="NBI74_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001174481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA accumulation protein YceD"
                     /protein_id="HCD1931482.1"
                     /translation="MQKVKLPLTLDPVRTAQKRLDYQGIYTPDQVERVAESVVSVDSD
                     VECSMSFAIDNQRLAVLNGDAKVTVTLECQRCGKPFTHQVYTTYCFSPVRSDEQAEAL
                     PEAYEPIEVNEFGEIDLLAMVEDEIILALPVVPVHDSEHCEVSEADMVFGELPEEAQK
                     PNPFAVLASLKRK"
     gene            22402..22986
                     /gene="yceF"
                     /locus_tag="NBI74_001116"
     CDS             22402..22986
                     /gene="yceF"
                     /locus_tag="NBI74_001116"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-methyl-GTP pyrophosphatase"
                     /protein_id="HCD1931483.1"
                     /translation="MPELILASTSPWRRALLEKLQISFECAAPEVDETPRSDESPRQL
                     VLRLAQEKAQSLASRYPDHLIIGSDQVCVLDGEITGKPLTEENARLQLRKASGNIVTF
                     YTGLALFNSANGHLQTEVEPFDVHFRHLSEAEIDNYVRKEHPLHCAGSFKSEGFGITL
                     FERLEGRDPNTLVGLPLIALCQMLRREGKNPLMG"
     gene            complement(23098..24057)
                     /gene="rluC"
                     /locus_tag="NBI74_001117"
     CDS             complement(23098..24057)
                     /gene="rluC"
                     /locus_tag="NBI74_001117"
                     /EC_number="5.4.99.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(955/2504/2580) synthase
                     RluC"
                     /protein_id="HCD1931484.1"
                     /translation="MKTETPSVKIVAITADEAGQRIDNFLRTQLKGVPKSMIYRILRK
                     GEVRVNKKRIKPEYKLEAGDEVRIPPVRVAEREEEAVSPHLQKVAALADVILYEDDHI
                     LVLNKPSGTAVHGGSGLSFGVIEGLRALRPEARFLELVHRLDRDTSGVLLVAKKRSAL
                     RSLHEQLREKGMQKDYLALVRGQWQSHVKSVQAPLLKNILQSGERIVRVSQEGKPSET
                     RFKVEERYAFATLVRCSPVTGRTHQIRVHTQYAGHPIAFDDRYGDREFDRQLTEAGTG
                     LNRLFLHAAALKFTHPGTGEVMRIEAPMDDGLKRCLQKLRNAR"
     gene            24630..27815
                     /gene="rne"
                     /locus_tag="NBI74_001118"
     CDS             24630..27815
                     /gene="rne"
                     /locus_tag="NBI74_001118"
                     /EC_number="3.1.26.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E"
                     /protein_id="HCD1931485.1"
                     /translation="MKRMLINATQQEELRVALVDGQRLYDLDIESPGHEQKKANIYKG
                     KITRIEPSLEAAFVDYGAERHGFLPLKEIAREYFPANYSAHGRPNIKDVLREGQEVIV
                     QIDKEERGNKGAALTTFISLAGSYLVLMPNNPRAGGISRRIEGDDRTELKEALASLEL
                     PEGMGLIVRTAGVGKSAEALQWDLSFRLKHWEAIKKAAESRPAPFLIHQESNVIVRAF
                     RDYLRQDIGEILIDNPKVLELARQHIAALGRPDFSSKIKLYTGEIPLFSHYQIESQIE
                     SAFQREVRLPSGGSIVIDSTEALTAIDINSARATRGGDIEETAFNTNLEAADEIARQL
                     RLRDLGGLIVIDFIDMTPVRHQRAVENRLREAVRQDRARIQISHISRFGLLEMSRQRL
                     SPSLGESSHHVCPRCSGTGTVRDNESLSLSILRLIEEEALKENTQEVHAIVPVPIASY
                     LLNEKRSAVNAIETRQDGVRCVIVPNDQMETPHYHVLRVRKGEETPTLSYMLPKLHEE
                     AMALPSEEEFAERKRPEQPALATFAMPDVPPAPTPAEPAAPVVAPAPKSAPATPAAPA
                     QPGLLSRFFGALKALFSGGEETKPSEQPTPKAEAKPERQQDRRKPRQNNRRDRNERRD
                     PRSERTEGSDNREENRRNRRQAQQQTAETRESRQQAEVTEKARTTDEQQTPRRERSRR
                     RNDDKRQAQQEVKALNVEEQSVQETEQEERVRPVQPRRKQRQLNQKVRYEQSVAEEAV
                     VAPVVEETVAAEPIVQEAAAPRTELVKVPLPVVAQTAPEQQEENNADNRDNGGMPRRS
                     RRSPRHLRVSGQRRRRYRDERYPTQSPMPLTVACASPELASGKVWIRYPIVRPQDVQV
                     EEQREQEEVQVQPMVTEVPVAAAAEPVVSAPVVEEVAEVVEAPVQVAEPQPEVVETTH
                     PEVIAAAVTEQPQVITESDVAVAQEVAEHAEPVVEPQEETADIEEVAETAEVVVAEPE
                     VVAQPAAPVVAEVTAEVETVAAVEPEVTVEHNHATAPMTRAPAPEYVPEAPRHSDWQR
                     PTFAFEGKGAAGGHTATHHASAAPARPQPVE"
     gene            complement(28010..28963)
                     /gene="flgL"
                     /locus_tag="NBI74_001119"
     CDS             complement(28010..28963)
                     /gene="flgL"
                     /locus_tag="NBI74_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
                     /protein_id="HCD1931486.1"
                     /translation="MRFSTQMMYQQNMRGITNSQAEWMKYGEQMSTGKRVVNPSDDPI
                     AASQAVVLSQAQAQNSQYTLARTFATQKVSLEESVLSQVTTAIQNAQEKIVYASNGTL
                     SDDDRASLATDIQGLRDQLLNLANTTDGNGRYIFAGYKTESAPFSEADGKYEGGAESI
                     KQQVDASRSMVIGHTGDKIFDSITSNAVAEPDGSASETNLFAMLDSAIAALKTPVADS
                     EADKETAAAALDKTNRGLKNSLNNVLTVRAELGTQLNELESLDSLGSDRALGQTQQMS
                     DLVDVDWNATISSYIMQQTALQASYKAFTDMQGLSLFQLNK"
     gene            complement(28975..30618)
                     /gene="flgK"
                     /locus_tag="NBI74_001120"
     CDS             complement(28975..30618)
                     /gene="flgK"
                     /locus_tag="NBI74_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HCD1931487.1"
                     /translation="MSSLINNAMSGLNAAQAALNTASNNISSYNVAGYTRQTTIMAQA
                     NSTLGAGGWVGNGVYVSGVQREYDAFITNQLRAAQTQSSGLTARYEQMSKIDNMLSTS
                     TSSLATQMQDFFTSLQTLVSNAEDPAARQALIGKSEGLVNQFKTTDQYLRDQDKQVNI
                     AIGASVDQINNYAKQIASLNDQISRLTGVGAGASPNNLLDQRDQLVSELNQIVGVEVS
                     VQDGGTYNITMANGYSLVQGSTARQLAAVPSSADPSRTTVAYVDGTAGNIEIPEKLLN
                     TGSLGGILTFRSQDLDQTRNTLGQLALAFAEAFNTQHKAGFDANGDAGEDFFAIGKPA
                     VLQNTKNKGDVAIGATVTDTSAVLATDYKISFDNNQWQVTRLASNTTFTVTPDANGKV
                     AFDGLELTFTGTPAVNDSFTLKPVSDAIVNMDVLITDEAKIAMASAEDAGDSDNRNGQ
                     ALLDLQSNSKTVGGAKSFNDAYASLVSDIGNKTATLKTSSTTQGNVVTQLSNQQQSIS
                     GVNLDEEYGNLQRFQQYYLANAQVLQTANAIFDALINIR"
     gene            complement(30684..31625)
                     /gene="flgJ"
                     /locus_tag="NBI74_001121"
     CDS             complement(30684..31625)
                     /gene="flgJ"
                     /locus_tag="NBI74_001121"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly peptidoglycan hydrolase FlgJ"
                     /protein_id="HCD1931488.1"
                     /translation="MISDSKLLASAAWDAQSLNELKAKAGEDPAANIRPVARQVEGMF
                     VQMMLKSMRDALPKDGLFSSEHTRLYTSMYDQQIAQQMTAGKGLGLAEMMVKQMTPEQ
                     PLPEESTPAAPIKFPLETVVRYQNQTLSQLVQKAVPRNYDDSLPGDSKAFLAQLSLPA
                     QLASQQSGVPHHLILAQAALESGWGQRQIRRENGEPSYNLFGVKASGNWKGPVTEITT
                     TEYENGEAKKVKAKFRVYSSYLEALSDYVGLLTRNPRYAAVTTAASAEQGAQALQDAG
                     YATDPHYARKLTNMIQQMKSISDKVSKTYSMNIDNLF"
     gene            complement(31625..32722)
                     /gene="flgI"
                     /locus_tag="NBI74_001122"
     CDS             complement(31625..32722)
                     /gene="flgI"
                     /locus_tag="NBI74_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415598.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HCD1931489.1"
                     /translation="MIKFLSALILLLVTTAAQAERIRDLTSVQGVRQNSLIGYGLVVG
                     LDGTGDQTTQTPFTTQTLNNMLSQLGITVPTGTNMQLKNVAAVMVTASLPPFGRQGQT
                     IDVVVSSMGNAKSLRGGTLLMTPLKGVDSQVYALAQGNILVGGAGASAGGSSVQVNQL
                     NGGRITNGAVIERELPSQFGVGNTLNLQLNDEDFSMAQQIADTINRVRGYGSATALDA
                     RTIQVRVPSGNSSQVRFLADIQNMQVNVTPQDAKVVINSRTGSVVMNREVTLDSCAVA
                     QGNLSVTVNRQANVSQPDTPFGGGQTVVTPQTQIDLRQSGGSLQSVRSSASLNNVVRA
                     LNALGATPMDLMSILQSMQSAGCLRAKLEII"
     gene            complement(32734..33432)
                     /gene="flgH"
                     /locus_tag="NBI74_001123"
     CDS             complement(32734..33432)
                     /gene="flgH"
                     /locus_tag="NBI74_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HCD1931490.1"
                     /translation="MQKNAAHTYAISSLLALSLTGCAWIPSTPLVQGATSAQPVPGPT
                     PVANGSIFQSAQPINYGYQPLFEDRRPRNIGDTLTIVLQENVSASKSSSANASRDGKT
                     NFGFDTVPRYLQGLFGNARADVEASGGNTFNGKGGANASNTFSGTLTVTVDQVLVNGN
                     LHVVGEKQIAINQGTEFIRFSGVVNPRTISGSNTVPSTQVADARIEYVGNGYINEAQN
                     MGWLQRFFLNLSPM"
     gene            complement(33486..34268)
                     /gene="flgG"
                     /locus_tag="NBI74_001124"
     CDS             complement(33486..34268)
                     /gene="flgG"
                     /locus_tag="NBI74_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HCD1931491.1"
                     /translation="MISSLWIAKTGLDAQQTNMDVIANNLANVSTNGFKRQRAVFEDL
                     LYQTIRQPGAQSSEQTTLPSGLQIGTGVRPVATERLHSQGNLSQTNNSKDVAIKGQGF
                     FQVMLPDGSSAYTRDGSFQVDQNGQLVTAGGFQVQPAITIPANALSITIGRDGVVSVT
                     QQGQAAPVQVGQLNLTTFMNDTGLESIGENLYTETQSSGAPNESTPGLNGAGLLYQGY
                     VETSNVNVAEELVNMIQVQRAYEINSKAVSTTDQMLQKLTQL"
     gene            complement(34440..35195)
                     /gene="flgF"
                     /locus_tag="NBI74_001125"
     CDS             complement(34440..35195)
                     /gene="flgF"
                     /locus_tag="NBI74_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgF"
                     /protein_id="HCD1931492.1"
                     /translation="MDHAIYTAMGAASQTLNQQAVTASNLANASTPGFRAQLNALRAV
                     PVEGLSLPTRTLVTASTPGADMTPGKMDYTSRPLDVALQQDGWLVVQTADGSEGYTRN
                     GSIQVDPTGQLTIQGHPVVGEAGPIAVPEGAEITIAADGTISALNPGDPANTVAPVGR
                     LKLVKATGSEVQRGDDGIFRLSAESQATRGPVLQADPTLRVMSGVLEGSNVNAVAAMS
                     DMIASARRFEMQMKVISSVDDNAGRANQLLSMS"
     gene            complement(35215..36420)
                     /gene="flgE"
                     /locus_tag="NBI74_001126"
     CDS             complement(35215..36420)
                     /gene="flgE"
                     /locus_tag="NBI74_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook protein FlgE"
                     /protein_id="HCD1931493.1"
                     /translation="MAFSQAVSGLNAAATNLDVIGNNIANSATYGFKSGTASFADMFA
                     GSKVGLGVKVAGITQDFTDGTTTNTGRGLDVAISQNGFFRLVDSNGSVFYSRNGQFKL
                     DENRNLVNMQGLQLTGYPATGTPPTIQQGANPTNISIPNTLMAAKTTTTASMQINLNS
                     SDSLPSVNAFDASNADSYNKKGSVTVFDSQGNAHDMSVYFVKTGDNNWDVYTLDSSDP
                     TGTANPATTLVFNANGVLTSDPTKDITTGAINGADPATFSLSFLNSMQQNTGANNIVA
                     TTQNGYKPGDLVSYQINDDGTVVGNYSNEQTQLLGQIVLANFANNEGLASEGDNVWSA
                     TQSSGVALLGTAGTGNFGTLTNGALEASNVDLSKELVNMIVAQRNYQSNAQTIKTQDQ
                     ILNTLVNLR"
     gene            complement(36445..37140)
                     /gene="flgD"
                     /locus_tag="NBI74_001127"
     CDS             complement(36445..37140)
                     /gene="flgD"
                     /locus_tag="NBI74_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook assembly protein FlgD"
                     /protein_id="HCD1931494.1"
                     /translation="MSIAVTTTDPTNTGVSTTSSSSLTGSNAADLQSSFLTLLVAQLK
                     NQDPTNPMENNELTSQLAQISTVSGIEKLNTTLGSISGQIDNSQSLQASNLIGHGVMI
                     PGTTVLAGTGSEEGAVTTTTPFGVELQQAADKVTATITDKNGAVVRTIDIGELTAGVH
                     SFTWDGTLTDGSTAPNGSYNVAISASNGGTQLVAQPLQFALVQGVIRGNNGNTLDLGT
                     YGTTTLDEVRQII"
     gene            complement(37152..37556)
                     /gene="flgC"
                     /locus_tag="NBI74_001128"
     CDS             complement(37152..37556)
                     /gene="flgC"
                     /locus_tag="NBI74_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HCD1931495.1"
                     /translation="MALLNIFDIAGSALTAQSQRLNVAASNLANADSVTGPDGQPYRA
                     KQVVFQVNAAPGAATGGVKVADVIESQAPDKLVYEPGNPLADAKGYVKMPNVDVVGEM
                     VNTMSASRSYQANVEVLNTVKSMMLKTLTLGQ"
     gene            complement(37560..37976)
                     /gene="flgB"
                     /locus_tag="NBI74_001129"
     CDS             complement(37560..37976)
                     /gene="flgB"
                     /locus_tag="NBI74_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HCD1931496.1"
                     /translation="MLDKLDAALRFQQEALNLRAQRQEVLAANIANADTPGYQARDID
                     FASELKKVMQRGRDATSVVALTMTSTQHIPAQALTPPSAELQYRIPDQPSLDGNTVDM
                     DRERTQFADNSLQYQMSLSALSGQIKGMMNVLQSGN"
     gene            38131..38790
                     /gene="flgA"
                     /locus_tag="NBI74_001130"
     CDS             38131..38790
                     /gene="flgA"
                     /locus_tag="NBI74_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation protein
                     FlgA"
                     /protein_id="HCD1931497.1"
                     /translation="MLTIKRSVAIIAILFSPLSTASNLTSQLHNFFSAQLAGVSDEVR
                     VSIRTAPNLLPPCEQPLLSMSNNSRLWGNVNVLARCGNDKRYLQVNVQATGNYVVAAM
                     PIVRGGKLEAGNVKLKRGRLDTLPPRTVLDINQLVDAVSLRDLSPDQPIQLTHFRQAW
                     RVKAGQRVNVIASGDGFSANAEGQALNNAAVAQNARVRMISGQVVSGVVDADGNILIN
                     L"
     gene            38866..39159
                     /gene="flgM"
                     /locus_tag="NBI74_001131"
     CDS             38866..39159
                     /gene="flgM"
                     /locus_tag="NBI74_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-28 factor FlgM"
                     /protein_id="HCD1931498.1"
                     /translation="MSIDRTSPLKPVSTVQPRETTDAPVTNTRAAKTTASTSTSVTLS
                     DAQAKLMQPGSSDINLERVEALKLAIRNGELKMDTGKIADALINEAQQDLQSN"
     gene            39164..39580
                     /gene="flgN"
                     /locus_tag="NBI74_001132"
     CDS             39164..39580
                     /gene="flgN"
                     /locus_tag="NBI74_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis chaperone FlgN"
                     /protein_id="HCD1931499.1"
                     /translation="MTRLAEILDQMSAVLNDLKTVMDQEQQHLSMGQINGSQLQWITE
                     QKSSLLATLDYLEQLRRKEPNTANSVDISQRWQEIAVKTQQLRQMNQHNGWLLEGQIE
                     RNQQALEMLKPHQEPTLYGANGQTSTPYRGSKKISI"
     gene            complement(39620..41155)
                     /gene="murJ"
                     /locus_tag="NBI74_001133"
     CDS             complement(39620..41155)
                     /gene="murJ"
                     /locus_tag="NBI74_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706990.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HCD1931500.1"
                     /translation="MNLLKSLAAVSSMTMFSRVLGFARDAIVARIFGAGMATDAFFVA
                     FKLPNLLRRIFAEGAFSQAFVPILAEYKSKQGEDATRVFVSYVSGLLTLALAVVTVAG
                     MLAAPWVIMVTAPGFADTADKFALTSQLLKITFPYILLISLASLVGAILNTWNRFSIP
                     AFAPTLLNISMIGFALFAAPYFNPPVLALAWAVTVGGILQLVYQLPHLKKIGMLVLPR
                     INFHDAGAMRVVKQMGPAILGVSVSQISLIINTIFASFLASGSVSWMYYADRLMEFPS
                     GVLGVALGTILLPSLSKSFASGNHDEYNRLMDWGLRLCFLLALPSAVALGILSGPLTV
                     SLFQYGKFTAFDALMTQRALIAYSVGLIGLIVVKVLAPGFYSRQDIKTPVKIAIVTLI
                     LTQLMNLAFIGPLKHAGLSLSIGLAACLNASLLYWQLRKQKIFTPQPGWMAFLLRLVV
                     AVLVMSGVLLGMLHIMPEWSLGTMPWRLLRLMAVVLAGIAAYFAALAVLGFKVKEFAR
                     RTV"
     gene            complement(41265..42188)
                     /locus_tag="NBI74_001134"
     CDS             complement(41265..42188)
                     /locus_tag="NBI74_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCD1931501.1"
                     /translation="MKKLRIGVVGLGGIAQKAWLPVLAAASDWTLQGAWSPTRAKALP
                     ICDSWRIPYADSLSSLAASCDAVFVHSSTASHFGVVSTLLNAGVHVCVDKPLAENLRD
                     AERLVELAARKKLTLMVGFNRRFAPLYGELKTQLATASSLRMDKHRTNSVGPHDLYFT
                     LLDDYLHVVDTALWLSGGNATLESGTLLTNESGEMLFAEHHFSAGPLQITTCMHRRAG
                     SQRETVQAVTDGALIDITDMREWREERGQGVVHKPIPGWQSTLEQRGFVDCARHFIEC
                     VQNQTVPQTAGEQAVLAQRIVDKIWRDAMSE"
     gene            complement(42190..42837)
                     /locus_tag="NBI74_001135"
     CDS             complement(42190..42837)
                     /locus_tag="NBI74_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceH family protein"
                     /protein_id="HCD1931502.1"
                     /translation="MKYQLTALEARVIGCLLEKQVTTPEQYPLSVNGVVTACNQKTNR
                     EPVMNLSESEVQEQLDNLVKRHYLRTVSGFGNRVTKYEQRFCNSEFGDLKLSAAEVAL
                     ITTLLLRGAQTPGELRSRAARMYEFSDMAEVESTLEQLANREDGPFVVRLAREPGKRE
                     SRYMHLFSGEVENPPAVTDMSNAVDGDLQARVEALEIEVAELKQRLDSLLAHLGD"
     gene            complement(42848..43432)
                     /gene="rimJ"
                     /locus_tag="NBI74_001136"
     CDS             complement(42848..43432)
                     /gene="rimJ"
                     /locus_tag="NBI74_001136"
                     /EC_number="2.3.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S5-alanine
                     N-acetyltransferase"
                     /protein_id="HCD1931503.1"
                     /translation="MFGYRSNVPKVRLTTDRLVVRLVHDRDAWRLADYYAENRHFLKP
                     WEPVRDESHCYPSGWQARLGMINEFHKQGSAFYFGLFDPDEKEIIGVANFSNVVRGSF
                     HACYLGYSIGQKWQGKGLMFEALSAAIRYMQRTQHIHRIMANYMPHNKRSGDLLARLG
                     FEKEGYAKDYLLIDGQWRDHVLTALTTPDWTPGR"
     gene            43668..44876
                     /gene="mdtH"
                     /locus_tag="NBI74_001137"
     CDS             43668..44876
                     /gene="mdtH"
                     /locus_tag="NBI74_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtH"
                     /protein_id="HCD1931504.1"
                     /translation="MSRVSQARNLGKYFLLIDNMLVVLGFFVVFPLISIRFVDQMGWA
                     AVMVGIALGLRQFIQQGLGIFGGAIADRFGAKPMIVTGMLMRAAGFATMGIAHEPWLL
                     WFSCLLSGLGGTLFDPPRSALVVKLIRPQQRGRFFSLLMMQDSAGAVIGALLGSWLLQ
                     YDFRLVCATGAVLFVLCAAFNAWLLPAWKLSTVRTPVREGMTRVMRDKRFVTYVLTLA
                     GYYMLAVQVMLMLPIMVNDVAGAPSAVKWMYAIEACLSLTLLYPIARWSEKHFRLEHR
                     LMAGLLIMSLSMMPVGMVSGLQQLFTLICLFYIGSIIAEPARETLSASLADARARGSY
                     MGFSRLGLAIGGAIGYIGGGWLFDLGKSAHQPELPWMMLGIIGIFTFLALGWQFSQKR
                     AARRLLERDA"
     gene            44940..45587
                     /gene="grxB"
                     /locus_tag="NBI74_001138"
     CDS             44940..45587
                     /gene="grxB"
                     /locus_tag="NBI74_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 2"
                     /protein_id="HCD1931505.1"
                     /translation="MKLYIYDHCPYCLKARMIFGLKNIPVELHVLLNDDAETPTRMVG
                     QKQVPILQKDDSRYMPESMDIVHYVDKLDGKPLLTGKRSPAIEEWLRKVNGYANKLLL
                     PRFAKSAFDEFSTPAARKYFVDKKEASAGNFADLLAHSDGLIKNISDDLRALDKLIVK
                     PNAVNGELSEDDIQLFPLLRNLTLVAGINWPSRVADYRDNMAKQTQINLLSSMAI"
     gene            45721..46281
                     /locus_tag="NBI74_001139"
     CDS             45721..46281
                     /locus_tag="NBI74_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCD1931506.1"
                     /translation="MNKFLFAAALIVSGLLVGCNQLTQYTITEQEINQSLAKHNNFSK
                     DIGLPGVADAHIVLTNLTSQIGREEPNKVTLTGDANLDMNSLFGSQKATMKLKLKALP
                     VFDKEKGAIFLKEMEVVDATVQPEKMQTVMQTLLPYLNQALRNYFNQQPAYVLREDGS
                     QGEAMAKKLAKGIEVKPGEIVIPFTD"
     gene            46387..47430
                     /gene="pyrC"
                     /locus_tag="NBI74_001140"
     CDS             46387..47430
                     /gene="pyrC"
                     /locus_tag="NBI74_001140"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HCD1931507.1"
                     /translation="MTAPSQVLKIRRPDDWHLHLRDGDMLKTVVPYTSEIYGRAIVMP
                     NLAPPVTTVEAAVAYRQRILDAVPAGHDFTPLMTCYLTDSLDPNELERGFNEGVFTAA
                     KLYPANATTNSSHGVTSVDAIMPVLERMEKIGMPLLVHGEVTHADIDIFDREARFIES
                     VMEPLRQRLTALKVVFEHITTKDAADYVRDGNERLAATITPQHLMFNRNHMLVGGVRP
                     HLYCLPILKRNIHQQALRELVASGFNRVFLGTDSAPHARHRKESSCGCAGCFNAPTAL
                     GSYATVFEEMNALQHFEAFCSVNGPQFYGLPVNDTFIELVREEQQVAESIALTDDTLV
                     PFLAGETVRWSVK"
     gene            47507..47752
                     /gene="dinI"
                     /locus_tag="NBI74_001141"
     CDS             47507..47752
                     /gene="dinI"
                     /locus_tag="NBI74_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein I"
                     /protein_id="HCD1931508.1"
                     /translation="MRIEVTIAKTSPLPAGAIDALAGELSRRIQYAFPDNEGHVSVRY
                     AAANNLSVIGATKEDKQRISEILQETWESADDWFVSE"
BASE COUNT        11560 a        12897 c        11859 g        11450 t
ORIGIN      
        1 attttttata acaatcatta attaatttaa aaacctttaa atttcagtta aatagaaagc
       61 gattattttt atggtttctg gactggtgaa cggaggtgat tatttttata ataacctttc
      121 attcacagtc accaggtaca acggcatcat ggcaactgac tcaacacaat gtgtaaaaaa
      181 aagccgtggc cgcccaaaag tgttcgacag ggatgccgcg cttgataagg ccatgaaatt
      241 gttctggcag cacggttatg aagcgacttc tcttgcggac ctcgttgaag cgaccggagc
      301 taaagcgccc acgctatacg cggaatttac caacaaagag gggttattcc gcgccgtact
      361 cgaccactat atcgatcgtt ttgccgccaa gcatgaagca cagctgtttt gtgaagagaa
      421 aagcgtggag tctgcgctgg ctgactattt tgctgccatc gccaactgct ttaccagcaa
      481 agacactccg gctggctgct tcatgatcaa caactgcacc accctctccc cggattcagg
      541 agatatcgcc aatacgctga aatcacgcca tgcgatgcaa gagcgcactt tgcagcagtt
      601 tttatgtcaa cgacaagcgc gcggggaaat cccgacccac tgtgacgtga cgcatctggc
      661 agaattcctt aattgtatta ttcaggggat gtcgatcagc gcacgcgaag gtgcatcact
      721 ggaaaaactg atgcagattg cccgaacgac tttgcgttta tggccggaac tgctgaaata
      781 ggtcgcaaaa aaagcccctc agcctgagcg aatgaggggc taaattgcag agtacagcct
      841 tgttatactt tgttattcaa aatcagctgc ccgttgctat ctagcgggat ctgcgtaccc
      901 ggatcgcggt ccatgcggat tttgccctgc tgatcgccaa tcttataggt cacatcataa
      961 ccgagcattt tttctgactt gtcatacacc gttttacaac gctgttgcgt agtcgtgtaa
     1021 gtatcgcttt cctggagaga gccctggatc tggttacctg catatccacc ccccagcgcc
     1081 cccacaacag tggcgacatc tttaccgcga ccacctccaa actgatgccc aatcacgccg
     1141 ccagcaacag cgccgagcac cgacccggta atgcgatttt catcctgcac cggtcgacga
     1201 tgggtcactg taacgttgcg acactcctga cgcggtgttt taaccgtttc cttgattggg
     1261 gttgcagaaa caacctgagc gtattgcggg ccacgttcaa acacgttcag actggccact
     1321 gccgctacgc ccagcgcagc tgcgacacca atcccgatac ccgccaacat tgacttattc
     1381 acgggaacac ctccttcttt gtagttatgc caatcatgca agcgaaggag aaaagcagct
     1441 atcagacaga agatgaaaag cgcgggaatg gctggaaata ctcagggatt cggaggagtc
     1501 tgaaggaatg cctgatgcga cgcttaacgc gtcttatcag gcctaccgct cgtaccattt
     1561 gtccagggcg gataaggcgt ttatgcccca tccgccagta caagtcgatt aatgcaactt
     1621 caaacgtgga cggataacgc ggttaatact ccccaccagc atcattaatc cggttttaaa
     1681 gtaaccatgc agcgcaatct ggtgcattcg gtacagcgag atgtatacaa agcgcgcaat
     1741 tcgtccttca atcatcattg agccgcgcgt caggttaccc atcaggctac cgactgtgga
     1801 aaagttcgac agcgatacca gcgaaccgtg atctttatac tgataatttt tcagcggctt
     1861 accgttcatc tgcgccagaa tgttgttcat tgcgcaggtt gccatctgat gtgcggcctg
     1921 agcgcgcggc ggaacaaagc ccccttccgg acgcgggcat gacgcgcagt cgccaatagc
     1981 gtatatgtct ggatcgcgag tggtttgcag cgtcggttcc accaccagct ggttgatacg
     2041 gttggtttca agaccaccga tatctttcag gaagtctggc gctttgatcc cggctgccca
     2101 caccatcaga tcagcctcaa tatattcgcc atctttagtg tgcaggccgc cttcatcagc
     2161 actggtgacc atggtttgcg tcagcacgcg aacgccaagt ttcgttaact cactatgggc
     2221 cgcagcagag atacgtggcg gcaatgcagg caaaatacgt tcgcccgctt ctaccagcgt
     2281 tacgttcagg gcttcgttgg tcagaccttt ataaccgtaa ctgtgcagtt gcttgaccgc
     2341 gttgtgcaat tcagcggaga gttctacacc cgtcgcgccg ccgccgacaa tcgcaatgtt
     2401 tactttgcca ttcgcgccca ggttggcgga gtatttcagg aacagattca gcatctcctg
     2461 gtggaagcga cgcgcctggt gcgggttatc gaggaaaatg cagttctctt tgacacctgg
     2521 tgtattgaaa tcgttagagg tgctacccag cgccattacc agggtgtcat aggcgatttt
     2581 acgttccgga accagcagtt cacctttctc gtcgcgcagt tctgcaatag tgattgtttt
     2641 cgcttcacga tcaatatcaa ttacggaacc cagctggaac tggaaaccat gattgcgcgc
     2701 atgggccaga tagctcaacg catcgacgcc ttcatcaagc gagccagtcg ccacttcgtg
     2761 cagcagcggt ttccacaagt ggctgtggtt acgatcgacc agcgtaattt tggctttttt
     2821 cttgcgtccc agcttatgcc ccagctgtgt tgccatttcc agcccaccag caccgccgcc
     2881 gacaatcaca atttttttca atggcgtagt caacgtgacc cccttaaatt tattaaccaa
     2941 ttgttaatta atggttatta acatagctct taattaacaa caggttatag caatgaaatt
     3001 gttcttcgct attgagaata acatgaatgg tgcattggtc ataccaaaat tgatgttaat
     3061 caagttttgt tgctgaaaag ataggcgact ttaccgggtg agatcaaaaa acagagtttg
     3121 ttgttcatta tttgagctga tttgtgagaa tgatatagag tgaggtgtgt atggagggcg
     3181 ttcggtaggc ctgataagag gcgcatcagg cattgatgct cgactgtcgg atgcggcgga
     3241 acgccttatc caacccacag aatccggagc gcatcaggta ccggcaaggt tacctgatgc
     3301 gacgagggca tttacccgag ggttttgaac gctttaatgc gctgtaaatg cggggagata
     3361 ttcttgaatt tgtgcgtctg ctcttcgtcc cagacaatct cgtaataatg atgcaactct
     3421 tcagatgtcc gctggctgtt aagcgcttca tcattacgcg acaaaatcac caggcaacga
     3481 tcgcgattct tctcacggaa gttggtcaca cacttagtgg caatatcggc atactcttcc
     3541 gggcgatcaa tcttcccttc catgttctca taagggaaca aattggggtt gaagatcacc
     3601 tggcggatat cacagagaaa accaatccgc tcagcccaat aaccgcccag accaacacca
     3661 caaatgagcg ggcgctcgtc gacgttcagt tgcaacattt tgtccacttc tttgagcaaa
     3721 tgctgcatat catgttttgg atgccgcgta ctgtagctta ttagccgtac atccgggtca
     3781 ataaactgca attgtaagac tttctcgtgg ttacccggac tgttagagtc aaaaccgtgc
     3841 aaatagataa tcatcgcttc ctcacataag ccagggttaa tgacctgcct tctcttcctg
     3901 ccagcgttca tgtaactgat tcagacgggc gctgatcgct ttccagcgag ccgagtccat
     3961 cagttcctgt cgcgaaaatg aacctttatg atacaaacgt gtaacacgtt ctgccttgat
     4021 cggtgacaga ttatctaaca cgctgacggc ccctttacga ttattgcaga ccaggatcat
     4081 atcgcaacct gcatccagtg atgcctgtcc acgctcggca taactgccca taatcgctgc
     4141 gccttccatc gataaatcgt cagagaaaat cacgccgtca aaacccaatt cctgacgcaa
     4201 aacggttttc agccagtagg gagaaccgct cgccggacgc ggatcaacat cactgtagat
     4261 cacatgcgca ggcataatgg cgtcgagttt attttcgcgg attaaggaac tgaagaccga
     4321 catatcttta gcgcgaatct ccgcttgcgg acgtgggtcg cacggtgttt ctttgtgtga
     4381 gtctgccgtt actgcaccgt gacctgggaa gtgtttcccg gtagttttca ttccggcttc
     4441 atgcatacca tcaataaacc ggctggcaat tgccagggct ttttgtggat cggcatgata
     4501 agaacgctcg ccaatcgccg cgctgatatg cccgacgtcc agcactggcg caaagctgat
     4561 atcaatatcc atagcaatca tttcgctggc catcaaccaa cctgcctctt gcgccagttt
     4621 tccgccttct tccattccca acagcgcagc gaatgattgt gccgctggca agcgggtaaa
     4681 accttcacga aaacgctgca cgcgtccacc ttcctgatct accgccacca ccagatgatt
     4741 tcgcgatgct gcgcggatct ggcgcaccag ttcacgtaac tgggcaggat catgatagtt
     4801 tcgcgtaaag agaatcagcc ctcccaccag cggatgcgcc agtatttcac gctcttccgc
     4861 gtccagttcg taaccttcga catccaacat tactggaccc acactgctct ccttattgtt
     4921 ttattaatag ctgccgccag gtgtcatcgg ccagcctgat aaattgttga tcgccggttt
     4981 gtcgccagcg gtactcaaac caccctgctt tgagcatcag cagccaggga aaccatcgcc
     5041 tgacctgacg ccataattgc gccggataaa tcttcgcgcg agtggcatag tcattgacca
     5101 attgccgatg ctggtcagta ttttccaccc acaccgccgc cagttccagc gcgatatcac
     5161 catctccggc atactcccag tcgatgagtt ttaaccctga cgcgctatgc actaaatttc
     5221 cggcgtggac atccatatgc aatggactta agcgtaaaag ccgtggttcc cgcgctttgc
     5281 gcagacgttt taacattcgc agccaaccca ctgtccgccg cgccggatcg ctttgctgcc
     5341 agtacagttc cagtaacggc aacagcgtta ttcgccagcc aaaacgcggt tgttgatgta
     5401 gataatacag caagcctgcc agttcgttgg tatccggcaa atacgttttt acctcgccgg
     5461 gcagatagtc aaccaccatc cagtcacgga gatataaatg cggcttcggt gcaatgcatg
     5521 cgggtagttg tgataaagcc cgatactggc gcaagaacgc ggactgcggc gcatcaggat
     5581 cgtgcggctg acgcacaaca aaacgctggc cctgatgttc aatgagaaaa ctcccgccac
     5641 taagcccact attaaacgtc gtgacgggat gaaactgcgg gaaaaagcgc gacagcaatt
     5701 cgtcgcgcgt gaggggatta ttgctgcgaa acggcacctt tacctgacca gataatttcg
     5761 cccgtctgca ccagcatcag ctgcatttgt agggtcggag cgttaacgtt gccagaggcg
     5821 ctggagtaca gcacgtaatg agcgccaaca ttgcgggcaa tgcctatggc tttactacgg
     5881 gtgcctaaac tgtcctgcgg cgacaaacct aattgttgct tagccatcga cagctgctgg
     5941 gcggaaacca gggtaaattt accgttatta gccagcgcat ttcgcagcgt ttcggtcgct
     6001 tctgcggcat tcaacgaacc gttagtacgg ttgttaacgc tatcaaccag caggacgcta
     6061 cccgcagtca cgccatcagc cccaagcatc ttactgacca tcggctgcat tgcgccattc
     6121 cagtcataat ggcgaatatg cggcgcaggc ggtgcagttt gatcttcgtg ctcaattggg
     6181 cctggctgct gcggtatcgt cggcaccgat ggcactgtgg ggacaggctg ttgtggctcg
     6241 gttggttgtt ccggcgctgg tttcacttct tctaccggtg caggttcacg ttgccccaca
     6301 cacccggcaa gaaacatcgc cagcgcggta atcaaggcgt agcgactcat ttttgtcatc
     6361 aagattcacc ccttacaaat atagataaag tctgactttg tgcgccccca gaaaattggc
     6421 gctgccgtac agcgtgaccg ccgaatgtgc gggaatggta acgctgcgtg gcctttccag
     6481 aggatgcatc tccagccctc tggcgtcata ccagtaaaaa cgataatgaa cggtaacggg
     6541 ttcttgcctt tcgttataaa gcgttgagga tgctgaaggt tgaatatcag atgtcgaaag
     6601 gacgggcttt tctgcactga tgcccgcagc caataaagat gactccatca ccagcgattg
     6661 ctcatcattc accggaattt ccggatgtga acgacagccc accagcacta acaacgccag
     6721 agacaccagc ccaaagcatc cttttctcat cgttacagac ctttatgcgc cagcattggt
     6781 cccagcggac ggccacccaa caagtgcata tggatgtggt aaacctcttg tccgccatgg
     6841 cggttggtgt tcatgatcag acgatagcca tcttcggcaa taccttcttg ctcagcaatt
     6901 tttgccgcta cggtgatcat gcgtcccagc gcctgctcat gctcagctga cacgtcgttc
     6961 acagtcggaa tgaggatatt cggaatgatc agaatatgcg ttggcgcttg cggcgaaata
     7021 tcgcgaaacg ccgttaccag atcatcctgg tagacgatat cggaggggat ctcacgacga
     7081 ataattttgc tgaatatagt ttcttctgcc acgacatttt cctttttcaa atagccatgc
     7141 gttgcgccat gctacgccgc gccacactgc gagtatagag tatggtcgag ttaaacctgc
     7201 tctttcaacc ttaactcacg atttcttaag caaaaaaccg ttttgctgcc gtatatatcg
     7261 ccattattcc catttctgcc tgcgaatgcg ctgaaaagac attttctgtt tggtttacac
     7321 accaaatatg aatgcgtata tttctcattt gcatttacat acaaaattat tcgcacacta
     7381 aatataaccg ctcatcaccc tgctccggca tgagcctgac gccaacataa accaagagat
     7441 ttcaaatgct ttcgacacaa tttaacaggg ataatcaaca tcaagccatc atcaaaccgt
     7501 cactccttgc cggttgcata gcactggcac tattaccttc cgccgctttt gctgcaccag
     7561 ccactgaaga aacggtgatt gttgagggtt cagccacagc cccagatgat ggcgaaaatg
     7621 attacaacgt aacgtctacc tctgcgggta caaaaatgca gatgactcaa cgtgatattc
     7681 ctcagtcggt cactattgtt agccagcagc ggatggaaga tcagcagtta caaacgctgg
     7741 gcgaagtgat ggaaaacacg ctggggatca gcaaaagtca ggcggattcc gatcgtgctc
     7801 tttattattc ccgcggattc cagatcgata actatatggt tgatggtatc cccacctatt
     7861 ttgaatcgcg ctggaatctg ggcgacgcac tttctgatat ggcactgttt gaacgcgtag
     7921 aagtagtgcg tggcgcgaca ggcctcatga ccgggacggg taatccatct gcggcaatta
     7981 atatggttcg aaaacacgcg accagtcgtg aatttaaagg cgatgtctcg gcggaatacg
     8041 gtagctggaa caaagaacgg tatgtggcgg atttacaaag cccactcacc gaagacggta
     8101 aaatccgcgc acgaattgtc ggcggctacc agaataacga ctcatggctg gaccgctaca
     8161 acagtgaaaa gaccttcttc tccggcattg tcgatgctga tttaggcgat cttacgatgc
     8221 tttcagccgg ttacgaatat cagcgtattg atgttaatag cccaacctgg ggcggtttac
     8281 cgcgctggaa tactgatggc agcagcaaca gttacgatcg cgcacgcagt accgcacctg
     8341 actgggcgta caacgataaa gagatcaaca aggtctttat gaccctgaag cagcggtttg
     8401 ctgatacctg gcaagcgacg ctgaatgcca cccactctga agtcgaattt gacagcaaaa
     8461 tgatgtatgt cgatgcctat gtaaacaaag cggatggtat gctggttggg ccatacagta
     8521 attatggccc tggttttgat tatgtcggag gcaccggttg gaacagcggc aaacgtaaag
     8581 ttgatgcgct ggatctgttc gctgacggta gttatgaatt gtttggtcgt cagcacaatt
     8641 taatgtttgg tggcagttac agcaaacaaa acaatcgtta cttcagttca tgggccaaca
     8701 ttttcccgga tgaaattggc agtttctaca actttaatgg caatttccca caaaccgaca
     8761 ggtcaccaca gagtctggcg caggacgata ccacacatat gaaatcgtta tatgctgcca
     8821 ctcgtgtcac ccttgccgat ccgctgcatc tgatcctcgg cgcacgttat accaagtggc
     8881 gggttgatac gctgacttac agcatggaga aaaaccacac cacgccttat gctggactgg
     8941 tgtttgacat caatgataac tggtcgacct acgccagcta tacctctatt tttcagccgc
     9001 aaaatgatcg tgacagttca ggcaaatatc tggcaccaat caccggtaac aactacgagc
     9061 tgggtctgaa atcggactgg atgaatagcc gtctgaccac cacgttagcc atcttccgta
     9121 ttgagcagga taatgtcgct cagtccaccg gtacacctat ccccggcagc aacggcgaaa
     9181 ccgcctataa agcggtggat gggacagtca gtaaaggtgt ggaatttgaa ctcaacggcg
     9241 caattaccga caactggcag ctgacgtttg gcgcaacgcg ctatattgca gaggataacg
     9301 aaggaaacgc cgttaatcct aatctgccac gcaccacggt taaaatgttc accagctatc
     9361 ggttgcctgt catgccagag ttgacggtcg gcggtggtgt taactggcaa aatcgcgtgt
     9421 ataccgacac cgtgacaccg tatggcacct tccgcgccga gcaaggtagc tacgcgctgg
     9481 tggatctctt cacccgctac caggtgacga aaaacttctc gttacagggg aacgtcaata
     9541 acctgttcga caaaacctac gataccaacg tggaaggttc tattgtctac ggcgcaccgc
     9601 gtaatttaag cattaccggc atgtatcaat tctgaataac gcctgtaaaa aaggcagcca
     9661 tctggctgcc ttagtctccc caacgtctta cggattagtg gttacggatg tactcatcca
     9721 tctcggtttt caggttatcg gatttagtac cgaaaatcgc ctgaacacca gaaccagcaa
     9781 ccactacgcc cgctgcgccc agtttcttca ggccagcctg atccacttta gacacatcag
     9841 caacgctgac gcgcagacgg gtaatacatg cgtcgaggtt agtaatgttt tctttaccac
     9901 caaatgcagc aaccagagcc ggtgccattt cgctggtacc tgtcgctttt gcgtcttcag
     9961 tcgcgtcttc acgacccggc gttttcagat ccagtgcttt aatcagcacg cggaagatgg
    10021 tgtagtaaac aatggcataa ccgataccga cgatcgggaa cagccacagt ttgctgctgt
    10081 taccagacag aacgatgaag tcgatcagac cgtgcgagaa cgacgtaccg tcacgcatcc
    10141 ccagaagaat acagattggg aatgccaggc ctgccagaat cgcgtggatg atgtacagga
    10201 tcggcgcaac gaacatgaag gagaactcga tcggctcggt gataccggtc aggaacgagg
    10261 tcagcgccgc ggagatcata ataccgccca ctttcgcgcg gttttctggt ttagcagagt
    10321 gccagatagc aattgcggca gctggcagac cgtacatttt gaacaggaag ccaccagaca
    10381 gtttacccgc agtcgggtca cccgccatat aacgcggaat gtcgccgtgg aaaacctgac
    10441 ctgctgcgtt ggtgtattca ccaatctgca tctggaaagg tacgttccag atgtggtgca
    10501 gaccaaacgg taccaggcaa cgttcgatga aaccgtaaat gccaaacgca actaccgggt
    10561 tctggtaagc agcccactga gagaaggtct ggattgcaga gccaataggc ggccaaatga
    10621 aggacagcac aacgccagta aagatggcag tcaggcctga aatgatcggc acaaagcgtt
    10681 taccggcaaa gaagccaaga tactcaggca gcttaatacg gtagaaacgg ttaaacatgt
    10741 acgctgcgat cgcaccggag ataatccccc cgagtacgcc agtatccgcc aggtgtttag
    10801 aggcgatttc ttcagcaggt aaatgcagta ccagtggcgc aaccacggcc atggttttaa
    10861 ccatgatgcc ataggcaaca actgcggcca gcgcggatac gccatcgtta ttggtaaagc
    10921 cgagggcgac accgatcgca aaaatcagtg gcatgtttgc aaagacggaa ccgcctgctt
    10981 ctgccataac atgcgataca acggcgggca gccagctgaa attcgcggaa ccgacgccca
    11041 gcagaatacc tgcgataggc agtacggata ccggcagcat cagcgattta ccgacctttt
    11101 gcaggttagc aaatgcattc ttaaacataa ttgagagtgc tcctgagtat gggtgctttt
    11161 tttacgttct cacgcgtggc aaggggggag tgcctcgccg tgtacagggc atctaagcgc
    11221 cctttattta ttacacagag taaaataatt cagtgccgat ttgtttgacg gctatcacgt
    11281 ttcaacttca gatcattgga gaagttgcac attttgacaa aacatatgtc acaatatttc
    11341 aaacgtggtt attcaccgcc cgattttcgg cggtgaactt tactcgtttt agccattaat
    11401 tacgagcttt aaaaaaactc attcaacgga tggattgaag gcgggaagca tcgatgtgaa
    11461 acagacgggc gaagttatcg gtggttacct gcgccagttc ttcaacggca acacctttca
    11521 acacagccat gtattctgca acgtcacgaa ccatcgcagg ttgattctct tttcctcgat
    11581 gcggtactgg cgcaaggtaa ggtgagtcag tttccaccag taaccgatcc agggggacat
    11641 aacgcgcagc atcgcgcagt tgctccgcat tacggaaggt cacaatgccg gaaaaggaga
    11701 tgtaaaatcc gagatccagt aatttacccg ccgtttctct gtcctctgta aaacagtgta
    11761 gtacgccacc gcaatccgtc actttttctt cgcgcagaat tgccagcgta tcggcgcggg
    11821 cgtcacgggt gtggacgata accggcttgt tcagttcacg accaatctgg atatgatgga
    11881 tgaaggactc ttgctgacgt actttagttt ccggcgtgta ataataatcc agcccggttt
    11941 cacccagcgc tacaacgccc tcttctgccg ccagacgacg taaatcttct acatcgtagg
    12001 gatcattctg gttgagtgga tgcacgccac aagaaaatac gacgttgtcg cgttcgccta
    12061 ccagatcccg catgtgtaaa taacccggta atgttgtggc gactgccaga caaaatttca
    12121 catcgcgtgc ggcggctttc gccagaacgt catccacgtc cttatgcaaa gattcataat
    12181 ccagaccatc gagatggcag tgtgagtcga ctaaaaacat gatgtctctc ttaaagatga
    12241 ggaaccggta gcacaacgcc cggttgcagg taatgctcaa tacgcagtaa aagatcggtg
    12301 atgagaagct cgcggttgat acctgtgaca gacattaact gttcacgaat gtggcaaaca
    12361 tcccccagta tagcctgcag gcgcgaggga gaaagatggt ttgccagttc gacgaccagc
    12421 cccggcacat caacattggt cacgtgcgca gcgccataat ggcgttttag cgcatccatc
    12481 agcaacgttg ccagccagtg taaacgcgcc ggagcttgtt cattattaag ggccgctagc
    12541 agcgaatacc agtcgcccga ttgcacgcca tatgccagcg cctgacacaa tgtttcacga
    12601 gcctgccagt tatctccctg aaacaacgcc agtgccgccc caggcgaacc ggcgcttaag
    12661 cgcaatgcgg caagtagtgc atcctgtgac attgtcactt cgcgtgaaag ccaggtcacg
    12721 gcgtactgtt ccggcggcgg cgcaaggtaa tgtaaccgac aacgactacg taatgttgcc
    12781 agtaaacgtt ctggctcgcg ggtagcaagg aaaaaccacg tctctgctgg tggctcttca
    12841 agcgttttca gcaatgcgtt agccgcggcg tcggttagta aggcagcatc ggttacccag
    12901 acaactttcg caccacctaa gcgtgcgtgc tcattcagct tttcggtgac ctcacgtacc
    12961 gcatcaatgc ccagcgcatt tttccctttt tcgggagcca gggtgtagta atcgggatgt
    13021 gtgccagcct gcatcaactg acatccacga cagtgaccgc aacttttgtg gccctgcggt
    13081 tgttggcaga gtaaataacg gctcagggcg tagattaaag catcatcgcc catgcccggt
    13141 aacgcctgaa tgagtagcgc atggtgacct cttccggcct gatagctggc taccagtttt
    13201 tcgaaatcag gtcgtaacca tggataccat ctcatgcgtc caactccttc acccaattgg
    13261 tcacggtagt gcggattgca tccatcacgg cctccagcgg ctgggtggca tcaatggtat
    13321 gaatgctttt atcttgtgct gccagttcca gatagcgggc gcgggtgcga ttaaagaaat
    13381 cgaatgactc ttgctcaata cgatccagct caccacgggc gcgagcacgt tttaagccga
    13441 cctctggggt gacatcaaga taaagcgtta agtcagggcg aaaatccccg agaacagcat
    13501 cacgcagtgt tgccagcata tgttggtcaa taccacgtcc tccaccctga taggcttgag
    13561 tggagagatc gtggcgatcg ccaatcaccc aggtgccgtt agccagcgca ggtttgatga
    13621 ccgtttctac cagttgaacg cgcgcggcat aaaacatcaa aacttcggct ttatcggtaa
    13681 tgacttcatc gcctaccgat ttgatatcca gcaccaggct tcttaacttt tcggcaagtt
    13741 gcgtaccgcc aggttcccga gtgaaaacca tgtcgcggat acccagttgc tcaagcgtct
    13801 caaccaccac gttacgcgcg gtagttttgc ctgcgccttc cagcccctca atgacgatat
    13861 acttactgcg catttttttc cttaagcact ttcagataat cctgcacaga cttgttatga
    13921 ctggcaagat tggtattaaa cgtgtgacca cctttaccat cggccacaaa atagagatac
    13981 ggcgtttttg ccggatgcgc agcagccttc agcgaatccg cccccggcgt cgctatcgca
    14041 cctggcggca gaccggtaat ggtataggtg ttatacgctg tcggcgtttc caggtctgca
    14101 cgagaaagtt tgccattata acgctctccc atcccgtaaa tcacggtcgg gtcggtctgc
    14161 aggcgcatac caatgcgtaa acggttgata aatactgagg caacctgatc gcgttcactg
    14221 gcaacggcgg tttctttttc gataattgat gccatcgtca ccaactggtt tttatcttta
    14281 taaggcagac cgtccgcacg cccttcccag gcgctatcga ccgctttcac cattttcttg
    14341 tgcgctcgtt tgagtaacgc gacatcggtg gtattggcgg tatacatcca ggtgtctggc
    14401 cagaaccaac cttcaatcca ctccgggttt tccagttcaa gtgcctgcgc tacggtggcg
    14461 tacttatcgt cgctcagcgt gtgcttgata tacggagcct cacgcaattg cttgaggtag
    14521 tcgctcagac gcatcccttc taccagtcgc agcgggaact gtgcttcttt accgctttcc
    14581 aacaatttca gcatctcgcg cacggtcatc tgcggtgtaa agcggtaagt cccggcttta
    14641 aagtgagaaa gatccggttc gatacgcagc agccattgga aaacccgtgg gcgattgatg
    14701 atcttatcgg cataaagctg ttcaccgagc gccagacgtc cggtccctgg cttcagggta
    14761 aatatcgtct cttctttgat aagcaatttg ctgtcggcaa gatggcgaac cttccagacg
    14821 cccacaccag cggcgatacc cagtaccacc agcaataaca agattatcaa taacactttt
    14881 ttcatgacta attcgggcgc tcacaaagtg gggctaaata ttcatataac gttgctgacg
    14941 aaaaggagac atcgccacag gcacgtacgg gcatcactgg cattaacgca ttacaaataa
    15001 ccatctcatc tgcctgcaac gcctcttcca gagaggcttg cacttcgaca agctgataag
    15061 aggattgtgc cagcaaacgg atacagaatt gtcgcataat gccgttaaca cctgcctgat
    15121 ccagtcgcgg cgtataaact acgttgccct tccgccagaa caaattagcc gcacagcatt
    15181 ccgtaaccca cccttcgctg tcaaggacca gcgcctcatc agcgtttgtc tgctcaagat
    15241 gagagcggat caatacttgc tcaagccgat taagatgttt aatacctgca agatgaggat
    15301 tgcgccccag ccgcaccggg cttagcgcca acgtcatccc ttcgttacgc aaacggtcgt
    15361 aatgtgcagg ataagccgta acggagagaa tccgcgttgc cggtccactg ttcaatgtgc
    15421 tgtaccctcg cccgccacta ccgcgactga tcacgacttt cagtacacca ttttgctgtt
    15481 ctgctgccag cgttttcatc tcttgttcaa gctgaggcca gaagtcacag gaaatcatca
    15541 accgctgaca agcatcctgt agtcgctgga tatgcgccga taacaaactg actttaccgt
    15601 cgataactct ggcggtggta aaacaaccat caccaaactg cgttgcccga tcgcttactg
    15661 ccagcgattc ctgcttatga ccgttaatta agaacatacc ggctccttat cagagaattg
    15721 ttagtgtggc agcatgttca ctacggaaca agtcggaata aaagctaaga aaaaaggccc
    15781 gcaagcggac cttttataag ggtggaaaat gacaatttag atctttttaa agatcaaaga
    15841 accattagtg ccaccgaagc cgaaggaatt acacagagtg tattccattc cgctaacctg
    15901 acgcgcttcg tgcggtacga aatccaaatc gcaaccttca tccgggttat ccaggttgat
    15961 ggttggcgga acagcctgat cgcgcagcgc aaggatggag tagatagatt ctactgcacc
    16021 cgccgcacct aacaggtgac cggtcataga tttcgtggag cttaccaaca cgcggcttgc
    16081 agcttcaccg aagatggttt tcaccgcctg cgcttcagct ttatcgccag ccggcgtaga
    16141 agtaccgtgc gcgttaacgt agccaatctg actcgcttca atgcctgcat cacgcagagc
    16201 atttgccatc gccagagctg cgcctgcgcc attttctggc ggtgacgtca tatgataagc
    16261 atcgctgctc ataccaaagc cgacgagttc agcgtaaatt ttcgcaccgc gttttttcgc
    16321 gtgttcgtac tcttcaagta ccagcatacc ggcaccatcg cccagtacga aaccatcacg
    16381 ctctttatcc cacgggcggc tcgccgcttg cgggttatca ttgcgggtag ataatgcgcg
    16441 tgccgcgcca aaaccaccaa cgcccagcgg cgtactggct ttctctgcgc cacctgcaac
    16501 catcacgtca gcatcgccat acgcgataat acgcgcagca tggccaatgt tgtgcacgcc
    16561 ggaagtacag gcggtcgcga tagagatgct tgggccacgc aggccataca tgatagtcag
    16621 atgacctgcc accatgttca caatcgttga cggaacgaag aatgggctga tcttacgtgg
    16681 accaccgttc atcagagatg tgtggttttc ttcgatcagt ccgaggccgc caatcccgga
    16741 gccaattgcg gcaccaatgc gggttgcgtt ctcttccgtt atttcaaggc cagaatcctg
    16801 catggcctga acgccagcga caattccata ttgaatgaag gcatccatct tgcgctgttc
    16861 tttgcgcgag ataatgtcct cacagttaaa atcctttact aagccagcaa atttcgttgc
    16921 ataggcgcta gtatcgaaat ggtcgattag gctgatgcca ctctgaccgg caagcagagc
    16981 tttccaggta gactctacgg tattgccgac aggagacaac atgcccagtc cggtcacaac
    17041 tacacgacgc ttagacacgt ttgtcctcca gggagggaaa aaatgattct agtgggacaa
    17101 aaagataaaa ctcaggcggt cgaacgaccg cctggagatg ttcacttacg cctggtggcc
    17161 gttgatgtaa tcaatggcag cctgaacggt ggtgattttc tcagcttctt cgtccggaat
    17221 ctcagtatca aactcttctt ccagagccat taccagctca acggtgtcaa gagaatccgc
    17281 gcccaggtct tcaacgaaag aagcattgtt ggtaacttct tcctgcttaa cgcccagctg
    17341 ttcgccgata attttcttaa cgcgttcttc gatagtgctc atactcttaa atttcctatc
    17401 aaaactcgct ttcgcgatgg ttttcgtagt gtataaaatg ttgaaaaatt tgcaactaaa
    17461 tcccggcagg tcttaccacg attttacgtt attttgaggc cttacccccc aataacgcaa
    17521 atcattttgc actaattatt actcataacc acgcagactg cgcgcaacat gagcattttg
    17581 tgcaaatcgc ggtcagacca tgtacatccc gccgttcaca tgcaaagttt cacccgtgat
    17641 gtaagctgct tcgtcggatg ccaggaatgc aaccgcgttg gcgatttcct gtgcgccgcc
    17701 gaggcgaccc gcaggaacct gcgccaggat acccgcacgc tggtcatcgc tcagcgcacg
    17761 tgtcatgtcc gtttcaataa agcccggagc aacaacgttt acagtaatac cgcgtgacgc
    17821 aacttcgcgc gccagtgatt tactgaagcc gatcaagccc gctttcgccg cagcgtagtt
    17881 ggcctgaccg ccatttccca tggtaccaac cacagaaccg atagtgataa tacgaccatg
    17941 acgctttttc atcatagcgc gcattaccgc ttttgacaga cggaaaacag atgaaaggtt
    18001 ggtttcgata atatcgttcc actcttcatc tttcattcgc attaacaggt tatcacgagt
    18061 gataccggca ttattgacca ggatatccac ttcaccaaat tctgcgcgaa ttttttccag
    18121 aacagattcg atagatgccg ggtcggtcac attcaacatc agacctttgc cgttggcacc
    18181 taaataatca ctgatcgcct gagcgccatt ttcactggtc gcagtgccaa taactttcgc
    18241 gccacgggct gcgagcgttt cagcaattgc gcggccaatt ccgcggcttg caccggttac
    18301 cagtgcgatt tttccttcaa aattcatgat tttcctcttt taaagctcga gcgccgctgc
    18361 catcgctgaa ggttcgttca gcgccgaggc ggtcagggtg tcgacaatgc gtttcgtcag
    18421 gccagtaagc actttgcccg ggccgacttc atagagatgt tctacgcctt gcgctgccat
    18481 gtattcaaca gacttcgtcc actgaaccgg gttatacaac tgacgtacca gtgcgtcacg
    18541 aatggcatca ccattggttt cgcatttcac atcaacgtta ttcacaacag gaactgttgg
    18601 tgcgttaaag gtgattttcg ctaattctac tgccagtttg tcggctgctg gtttcatcag
    18661 cgcacagtga gacggtacgc tcactggtag cggcagagcg cgttttgcgc ccgcagcttt
    18721 acaagcagca cccgcacgct caaccgcttc tttatgaccg gcaataacca cctgtcccgg
    18781 agagttaaag tttaccggag aaacgacctg accttctgca gcttcttcac acgctttcgc
    18841 aatagacgca tcatccagac cgatgattgc cgccatagca cccgttcctt ccggtacggc
    18901 ttcttgcatg aacttgccgc gcatctcaac cagacgcacc gcatcagcga aatcaatcac
    18961 accagcgcaa accagcgcgg agtattcccc caggctgtga ccggccatca ttgccggtgc
    19021 tttaccgccc tgctgctgcc atacgcgata cagcgcaaca gatgcagtca acagcgctgg
    19081 ctgggtttgc caggttttat tcagttcttc agctggcccc tgctgggtca gcgcccacag
    19141 gtcgtagccc agcgccgcag aagcttcagc aaacgtttct tcgacaattg gatagctcgc
    19201 cgccatatca gccagcattc caacggtttg agaaccctgt ccagggaaca caaatgcaaa
    19261 ttgcgtcatg ttttaatcct tatcctagaa acgaaccagc gcggagcccc aggtgaatcc
    19321 accgccaaag gcttcaagca gaaccaactg ccccggctta atgcgcccgt cgcgtacagc
    19381 ttcatccaga gcgcacggga cagaggccgc agaggtatta ccgtggcgat ccagcgtcac
    19441 cacgacattg tccatcgaca tgccgagttt tttcgctgtt gcactgataa tacgcaggtt
    19501 agcctgatgc ggaaccagcc agtccagttg agaacggtca agattattcg ccgtcagcgt
    19561 ctcatcaacg atgtgcgcca gttctgttac cgcaaccttg aagacttcgt tgcccgccat
    19621 cgtcagatga attgaattct ctggattcac acggtcagca ttaggcagcg tcagcaactc
    19681 accatagcta ccgtcggcat gcagatgggt ggagatgatt cccggctctt cagaggcagc
    19741 cagcaccgca gcgcccgcgc catcgccaaa aataataata gtcccacgat ctgttggatc
    19801 gcaggtgcgc gccagtacat cggaaccgac gaccagagca tacttcaccg ccccagattt
    19861 cacgtattga tcggctacgc ttaatgcata ggtgaaacct gcgcaggctg ctgcaacgtc
    19921 aaatgccggg caacctttaa tgcccagcat gctttgaatc tgacaagctg cgctcgggaa
    19981 agcgtgcgta gcagaagtcg ttgccacaac gatcaggcca atctggtctt tctcaatgcc
    20041 cgccatctca attgcgcgtg tcgccgcttc aaagcccatg gttgaaacgg tttcgtttgg
    20101 cgcggcaatg tggcgttcgc ggataccggt acgagtgaca atccactcgt cagaggtgtc
    20161 caccattttt tccaaatcgg cgtttgtccg cacttgttcg ggcagatagc tgccagtacc
    20221 aataatcttc gtatacatgt acgctcagtc acttttcggt tatataccgt cacttgcaaa
    20281 ctgcgagttc gctggcagcg tcctgctacc gcagagttcc gcttttgcca ccgtccagca
    20341 gctcaaaacc agctgggtat acagattcca ggcgagcggc aattcgctga ggaacttgtc
    20401 gctgcaccgc ctgcactgcc tgttcaatcg cgaccgcaaa agctcgctga ttggctgcac
    20461 catgactttt tatcaccgtg ccgcgcaatc ctaacagaca ggcgccgtta tactggtcgg
    20521 ggttgaggtg actgaatcgc ctcgtcaggc tcttttgtag ccaacgcttt aataacagta
    20581 gccaccacga ccgttttttc ccttcaccct gagatttcag cagagaaagg aacatcctga
    20641 caacaccttc catcgtcttt aatgtgacat ttcctgtaaa gccgtcacaa accagcacat
    20701 ctgtcttgcc agttaacaac tcattggctt caagatagcc gatataattg atagaaggga
    20761 ttgttttaag caccgctgag gcatcccgaa tactgtcgag accctttact tcttcttcac
    20821 caatattgag caacgccacg cgaggattag gaatttccac cacctcttca gccagaactg
    20881 agcccataat ggcaaattgc accaacattg tactgtcaca atcgacgttg gcccctaaat
    20941 cgaggaccac cgttttgccc ttttgctgat gcggtaatac cgtcaccagc gccggacgct
    21001 caatcccctc caggggcttg agtaataatt ttgccagccc cattagcgcc ccggtattac
    21061 cggcactgac acaggcttgc gctcgacctt ctttcaccag ctccagggcc acgcgcattg
    21121 aactcccacg actggcgcgg atagcttgcg aaggccgggc atcactggcg ataactgact
    21181 gcgcaggaat aatctgcaga cgcgaacgtt gttcaaagtc agctttagca agtaatggcg
    21241 tgatggcgtc gggattgccg actaaaagaa gagtgagttg cgaattagaa ttcagtgcct
    21301 gcaatgctgc aggcactgtc acggaagggc caaaatcccc tcccatgaca tctaacgcca
    21361 gggttagacg tgtcaaggta tcgtcgccgc ccggtttggt ctttccccag ttgcctgggg
    21421 aaaatcctca ctaagcttca tcacgcagac gcgtaattac ttagcgatga ccttgcggcc
    21481 gcggtagtaa ccgtcggcag tgatgtggtg acgcaggtgt ttttcaccag aagttttgtc
    21541 tacagacagg ctggtgactg cggtcagcgc gtcatgggaa cgacgcatgc cacgtttgga
    21601 acgggttggt ttattctgtt gtacggccat ggaccttact cctcaattac ggtttatccc
    21661 cgatccaacg gggagcacca attacttacg ctttaagctg gctaatacgg caaatgggtt
    21721 tggcttttgc gcttcttcag gcagttcacc aaagaccatg tccgcttcgg acacttcaca
    21781 gtgttcagaa tcatgcaccg gaactaccgg caaggcgagg atgatttcat cttcaaccat
    21841 tgcaagcaga tcgatttcac cgaattcgtt aacctcaatc ggttcatacg cttccggcag
    21901 tgcttcagcc tgttcgtctg aacgcacagg actaaaacaa tacgttgtgt agacctgatg
    21961 agtaaacggc ttcccgcaac gctgacactc gagcgttacc gtcaccttcg catcgccgtt
    22021 taacactgcg agacgttggt tatcgatagc gaacgacatg gagcattcca catcactgtc
    22081 cacactgact acggattcgg cgacgcgctc aacctgatca ggagtataga taccctggta
    22141 atcaaggcgt ttttgagccg tacgaaccgg atcgagagtc aggggtaatt ttaccttttg
    22201 catagggcgc gcatattaac tttgtaacgt catagagtca aagaaaaagg cagcctgtgg
    22261 ttgccttttg ccaataattc gcacacattg cgggttactg ctttattttc gttcagtgct
    22321 gataacgaac atcaacctgc aaaacgaagc gcatagttta aaatggctat catcaatacg
    22381 ctatatctga tggaaaaaaa tatgcctgaa cttattttag cctccacctc gccctggcgt
    22441 cgcgctcttc tggaaaaact gcaaatctct tttgaatgtg cagcacccga ggtcgacgaa
    22501 actccacgca gcgacgaatc accgcgacag ttggtgcttc gactggcaca agaaaaagcg
    22561 caatctctgg cgtcacgtta tccggatcat ttaattattg gttcagacca ggtatgtgtt
    22621 cttgatggcg aaatcaccgg taaaccgtta acggaagaaa atgcccgtct gcaattacgc
    22681 aaagccagcg gcaatatcgt caccttctat actggactgg cgttgtttaa ttcggcgaat
    22741 gggcatctac aaacagaagt ggagcctttt gacgtccatt tccgtcacct gagcgaggcg
    22801 gagattgata attacgtgcg taaagagcat cccctgcact gcgcgggtag ctttaagagt
    22861 gaaggatttg gcattacgct gtttgagcgt ttagaggggc gtgatcctaa cacgctggtt
    22921 ggtttaccgc taatcgcgct gtgtcagatg ttacgtcggg aaggaaaaaa cccgctgatg
    22981 ggatagtttt ttgcgccgga tgcggttacg tcgcatccgg caatcggtgc tggcggcctg
    23041 atgcggcata aacgccttat caggccaacc gcccattatc atgcttatat gctcgtatta
    23101 gcgcgcgtta cgcagttttt gcaaacaacg cttcaaacca tcatccatcg gcgcttcgat
    23161 acgcatcacc tcaccggtcc ccggatgggt aaacttcaac gctgcggcgt gcaggaacag
    23221 acgatttaat cccgtgcctg cttcagtgag ctgtctgtca aattcacggt caccgtagcg
    23281 atcgtcaaag gcaatcggat gacccgcata ttgtgtatgc acacggatct gatgagtacg
    23341 ccctgttacc gggctacaac gcaccagggt ggcaaatgca tagcgctctt ccactttaaa
    23401 gcgtgtttcc gacggtttgc cttcctgact cacacgcacg atacgttcgc cgctttgcag
    23461 aatatttttc agtaacggcg cttgaacgct cttcacatgc gactgccact gaccgcgcac
    23521 cagcgccagg taatcttttt gcatcccttt ttcacgtaat tgctcatgca gagaacgcaa
    23581 cgccgagcgt tttttcgcta ccagcaacac acctgaggtg tcccggtcaa gacgatgaac
    23641 cagttcaagg aaccgcgctt ccgggcgcaa cgcccgcaaa ccttcgataa cgccgaagct
    23701 taaaccacta ccgccatgta ccgccgtacc ggaaggttta ttcagcacca ggatatggtc
    23761 atcttcatat aagatgacgt ccgccagcgc cgccaccttt tgcagatgtg gcgaaaccgc
    23821 ctcttcttcc cgttcagcaa cgcgaaccgg tgggatacgc acctcatcac ccgcttcgag
    23881 tttatattca ggcttaatac gttttttgtt cacccgcact tcgcctttac gcaaaatacg
    23941 gtaaatcata ctttttggta cacctttcaa ttgggtacgc aaaaagttat cgatacgttg
    24001 ccccgcttcg tcagcggtga tagcaacaat ttttacggat ggagtctctg ttttcatggt
    24061 gggcgattct aattagccaa caggattcgc gccactcatt tttctatgct tatatttagt
    24121 tttgcacctt attacttcac tacatgatca ctttattgat ggttattaaa ccaatcacca
    24181 gcaagaagtg aaaaaactgt gagtaagcgg gtgataaatg gtaaaagtca tcttgctata
    24241 acaaggcttg cagtggaata atgaggccgt ttccgtgtcc atccttgtta aaacaagaaa
    24301 ttttacggaa taacccattt tgcccgaccg atcatccacg cagcaatggc gtaagacgta
    24361 ttgatctttc aggcagttag cgggctgcgg gttgcagtcc ttaccggtag atggaaatat
    24421 ttctggagag taatacccag tctgtttctt tgataattgc gctgtttttc cgcatgaaaa
    24481 acgggcaacc gacactctgc gcctctttga gctgacgata accgtgaggt tggcgacgcg
    24541 actagacacg aggccatcgg ttcacacccg gaaaggcgtt actttgcccg cagcttagtc
    24601 gtcaatgtaa gaataatgag taagttacga tgaaaagaat gttaatcaac gcaactcagc
    24661 aggaagagtt gcgcgttgcc cttgtagatg ggcagcgtct gtatgacctg gatatcgaaa
    24721 gtccagggca cgagcagaaa aaggcaaaca tctacaaagg taaaatcacc cgcattgaac
    24781 cgagtctgga agctgctttt gttgattacg gcgctgaacg tcacggtttc ctcccactaa
    24841 aagaaattgc ccgcgaatat ttccctgcta actacagtgc tcatggtcgt cccaacatta
    24901 aagatgtgtt gcgtgaaggt caggaagtca ttgttcagat cgataaagaa gagcgcggca
    24961 acaaaggcgc ggcattaacc acctttatca gtctggcggg tagctatctg gttctgatgc
    25021 cgaacaaccc gcgcgcgggt ggtatttctc gtcgtatcga aggcgacgac cgtaccgaat
    25081 taaaagaagc actggcaagc cttgaactgc cggaaggcat ggggcttatc gtgcgcaccg
    25141 ctggcgtcgg caaatctgct gaggcgctgc aatgggattt aagcttccgt ctgaaacact
    25201 gggaagccat caaaaaagcc gctgaaagcc gcccggctcc gttcctgatt catcaggaga
    25261 gcaacgtaat cgttcgcgca ttccgcgatt acttacgcca ggacatcggc gaaatcctta
    25321 tcgataaccc gaaagtgctc gaactggcac gtcagcatat cgctgcatta ggccgcccgg
    25381 atttcagcag caaaatcaaa ctgtacaccg gcgagatccc gctgttcagc cactaccaga
    25441 tcgagtcgca gatcgagtcc gccttccagc gtgaagttcg tctgccatcc ggtggttcca
    25501 ttgttatcga cagcaccgaa gcgttaacgg ccatcgacat caactccgca cgcgcgaccc
    25561 gcggcggcga tatcgaagaa accgcgttta acaccaacct cgaagctgcc gatgagattg
    25621 ctcgtcagtt gcgcctgcgt gacctcggcg gcctgattgt tatcgacttc atcgacatga
    25681 cgccagtacg ccaccagcgt gcggtagaaa accgtctgcg tgaagcggtg cgtcaggacc
    25741 gggcgcgtat tcaaatcagc catatttctc gctttggcct gctggaaatg tcccgtcagc
    25801 gcctgagccc atcactgggt gaatccagcc atcacgtctg cccgcgctgc tccggtaccg
    25861 gtaccgttcg tgacaacgaa tcgctgtcgc tctctattct gcgtctgatc gaagaagaag
    25921 cgctgaaaga gaacacccag gaagttcacg ccattgttcc tgtgccaatt gcttcttacc
    25981 tgctgaatga aaaacgttct gcggtaaatg cgattgaaac tcgtcaggac ggcgtacgct
    26041 gtgtgattgt gccaaacgat cagatggaaa ccccgcacta ccatgtgctg cgcgtgcgca
    26101 aaggggaaga aaccccaacc ttaagctaca tgctgccgaa gctgcatgaa gaagcgatgg
    26161 cgctgccgtc tgaagaagag ttcgctgaac gtaagcgtcc ggaacaacct gcgctggcaa
    26221 cctttgccat gccggatgtg ccgccagcac caacgccagc tgaacctgcc gcgcctgtcg
    26281 tagccccagc acctaaatct gcaccggcaa caccagccgc tcctgcccaa cctgggctgt
    26341 tgagccgctt cttcggcgca ctgaaagcgc tgttcagcgg tggtgaagaa accaaaccgt
    26401 ccgagcaacc aacaccgaaa gcagaagcga aaccggaacg tcaacaggat cgtcgcaagc
    26461 ctcgtcagaa caaccgccgt gaccgtaatg agcgccgcga ccctcgtagt gaacgtactg
    26521 aaggcagcga taatcgcgaa gaaaaccgtc gtaatcgtcg ccaggcacag cagcagactg
    26581 ccgagacgcg tgagagccgt cagcaggcag aggtaacgga aaaagcgcgt accaccgacg
    26641 agcagcaaac gccgcgtcgt gaacgtagcc gccgccgtaa cgatgataaa cgtcaggcgc
    26701 aacaagaagt gaaggcgctg aatgttgaag agcaatctgt tcaggaaacc gaacaggaag
    26761 aacgtgtacg tccggttcag ccgcgtcgta aacagcgtca gctcaatcag aaagtgcgtt
    26821 acgagcaaag cgtagccgaa gaagcggtag tcgcaccggt ggttgaagaa actgtcgcag
    26881 ccgaaccaat tgttcaggaa gcggcagccc cacgcacaga attggtgaaa gttccgctgc
    26941 cagtcgtagc gcaaactgca ccagaacagc aagaagagaa caatgctgat aaccgtgaca
    27001 acggtggcat gccgcgtcgt tctcgccgct cgcctcgtca cctgcgcgta agtggtcagc
    27061 gtcgtcgtcg ctatcgtgac gagcgttatc caacccagtc gccaatgccg ttgaccgtag
    27121 cgtgcgcgtc tccggaactg gcctctggca aagtctggat ccgctatcca attgtacgtc
    27181 cgcaagatgt acaggttgaa gagcagcgcg aacaggaaga agtacaagtg cagccgatgg
    27241 tgactgaggt ccctgtcgcc gccgctgccg aacctgttgt tagcgcgcca gttgttgaag
    27301 aagtggctga agtcgtagaa gcccccgttc aggttgccga accgcaaccg gaagtggttg
    27361 aaacgacgca tcctgaagtg attgctgccg cggtaactga acagccgcag gtgattaccg
    27421 agtctgatgt tgccgtagcc caggaagttg cagaacacgc agaaccggtg gttgaaccgc
    27481 aggaagagac ggcagatatt gaagaagttg ccgaaactgc tgaggttgtg gttgctgaac
    27541 ctgaagttgt tgctcaacct gccgcgccag tcgtcgctga agtcacagca gaagttgaaa
    27601 cggtagctgc ggttgaacct gaggtcaccg ttgagcataa ccacgctacc gcgccgatga
    27661 cgcgcgctcc ggcaccggaa tatgttccgg aagcaccgcg tcacagtgac tggcagcgcc
    27721 ctacttttgc cttcgaaggt aaaggtgccg caggtggtca tacggcaacg catcatgcat
    27781 ctgccgctcc tgcgcgtccg caacctgttg agtaatagtt agctcaagta atcaagccct
    27841 ggtaactgcc agggcttttt tatttcatct ttgagtcaac atattaaaat tcatcaaatg
    27901 gcatccttgc taaccaacaa tacaaaatag gcaaaaaaat gcccgctccg gcgatcccag
    27961 agcgggcaga caaaacatac ccagtttcat gatatgtttt aaagcgagct tatttgttga
    28021 gctggaagag cgacaatccc tgcatatcgg taaatgcttt atacgatgcc tgcaatgccg
    28081 tttgctgcat gatgtaagat gaaatagttg cattccagtc cacatcaacc agatcgctca
    28141 tctgctgcgt ttgccctaaa gcgcgatcgc tacctaatga atccagcgac tccagttcgt
    28201 tcagttgcgt gcctaattcc gcgcgaacag tcagcacatt gttcagcgag tttttcagtc
    28261 cgcggttggt tttatccagt gccgcagcgg cggtttcttt atccgcttcg ctatccgcga
    28321 ccggcgtttt cagggctgcg atggcgctat ccagcatggc aaaaagattg gtttcagaag
    28381 cgctaccgtc tggttccgct accgcgttgc tagtaatact gtcgaaaatt ttgtcgcccg
    28441 tatgtcctat caccatcgaa cgcgaagcat cgacctgttg tttaatactt tctgctccgc
    28501 cttcgtattt gccatccgcc tcgctaaacg gtgcggattc tgttttataa ccggcaaaaa
    28561 tgtagcgccc gttaccgtca gtggtgttcg ccagattcag caactggtca cgaagcccct
    28621 gaatatccgt agccagcgag gcccggtcat cgtcactcaa ggtgccattg ctggcgtaga
    28681 caattttttc ctgagcattc tgtatagcgg ttgtgacctg gctaagtaca ctctcttcca
    28741 gtgacacttt ttgagtggcg aaagtacgcg ccagcgtgta ctggctgttt tgcgcctgtg
    28801 cctgggagag aacaacagct tgtgatgcag caatgggatc atctgaaggg ttaacgactc
    28861 gcttacccgt cgacatctgt tcgccgtact tcatccattc tgcctgagaa ttggtgatgc
    28921 cgcgcatgtt ttgctggtac atcatctggg tactgaaacg cattttatct ccccttagcg
    28981 aatgttaatc agcgcatcaa aaatcgcgtt tgccgtctgc agaacctgcg cattcgcgag
    29041 gtaatactgc tgaaaacgtt gcagatttcc gtactcctca tcgagattga caccggaaat
    29101 cgactgctgc tgattggaaa gctgcgtcac cacattacct tgagtggtgc tactggtttt
    29161 caacgtcgcg gttttattac cgatatcact cactaacgag gcataagcgt cgttaaagga
    29221 tttcgcaccg cccaccgttt tactgttgct ttgcagatcg agcagggcct gaccgttgcg
    29281 gttgtcgcta tcacccgcat cttctgcgct cgccatggcg attttcgctt cgtcggtgat
    29341 taatacatcc atgttgacga tggcgtcact taccggtttc agcgtgaagc tgtcgttaac
    29401 ggcaggcgtt cctgtaaacg tcaactccag accatcaaat gccactttac cgttggcatc
    29461 cggcgtcacc gtaaaagtgg tattgctggc aaggcgggtg acctgccact gattattatc
    29521 gaacgagatt ttgtaatctg tcgccagtac cgcggaggta tcagttaccg ttgcaccgat
    29581 cgcaacgtca cctttgtttt tcgtgttttg cagaaccgcg ggcttaccga tagcaaagaa
    29641 atcttcaccg gcatcgccgt tagcatcaaa tccggctttg tgttgggtgt tgaaagcctc
    29701 ggcaaatgcc agcgccagtt gtccaagcgt attacgcgtc tggtccagat cctgagaacg
    29761 gaatgtcaga atgccgccca gcgacccggt attcagtaat ttctccggga tctcaatatt
    29821 gcctgccgtc ccatcaacat aagcgacagt cgtacgagaa gggtcggcgc tggaaggaac
    29881 tgccgcaagt tgccgcgccg tacttccctg aaccagtgag tagccattgg ccatcgtgat
    29941 gttataagtg ccaccatcct gaacactgac ttctacgcca acaatctggt ttaattcgct
    30001 caccagttga tcgcgttgat ccagcagatt gttaggtgac gcccctgccc ccacgcctgt
    30061 caggcgggag atctgatcgt tcaggctggc gatttgttta gcgtaattgt tgatctgatc
    30121 aacactggca cctatcgcga tattgacctg tttgtcctgg tcgcgcagat attgatcggt
    30181 ggttttaaac tgattcacca acccttctga tttcccaatc agcgcctggc gcgctgccgg
    30241 atcttccgcg ttactcacca gcgtttgcag gctggtgaag aaatcctgca tctgtgttgc
    30301 cagcgaagag gtactggtgg agagcatatt gtcgattttc gacatctgct catagcgggc
    30361 agtcaggccg ctactttgcg tctgcgccgc acgtaactgg ttggtaataa acgcatcata
    30421 ctcacgctgc acaccagaaa cgtagacgcc attgccaacc cagccgccag cgcccaacgt
    30481 gctattggcc tgcgccataa tagtggtttg gcgggtatat ccggcaacgt tatagctgga
    30541 gatattatta cttgccgtat ttaacgccgc ctgggccgcg ttcagtccgc tcatggcgtt
    30601 attgatcaag ctggacatgg aggttcctta taagccttca attacagagt attatcggca
    30661 gcgccccgcc ggacttgagt tattcagaac agattatcaa tgttcatgct gtaggttttg
    30721 ctcaccttgt cgcttatcga tttcatctgc tgaatcatgt tggtgagttt gcgggcatag
    30781 tgaggatcgg tggcatagcc cgcgtcctgt agggcctgcg ccccctgctc cgcactcgcg
    30841 gcggtcgtca cggcggcgta gcgcgggtta cgcgttaaca gcccaacgta atccgacaat
    30901 gcttccagat acgagctgta gacccgaaac ttcgctttta ctttcttcgc ttcgccattt
    30961 tcatattcag tcgtggtgat ttcagtgact ggccctttcc agttgccaga ggctttgaca
    31021 ccaaacaggt tatagctcgg ctcgccgttt tcacggcgga tttgccgttg tccccagcca
    31081 gattccagcg ccgcctgagc gaggatcaaa tgatgtggca caccactttg ctggcttgcc
    31141 agttgggcgg gcagcgagag ttgcgcgagg aatgctttac tgtcacccgg cagcgaatca
    31201 tcgtagttac gtggtacggc cttttgcacc agctgcgaaa gcgtctgatt ttgataacgc
    31261 accacggttt cgagcgggaa ttttatcggt gctgctggcg tggactcctc tggcaatggt
    31321 tgttctggcg tcatctgttt aaccatcatc tctgccagcc ccagaccttt gcccgccgtc
    31381 atctgttggg caatctgctg gtcatacata ctggtataca ggcgagtgtg ctcgctgctg
    31441 aacaggccat cttttggtaa agcgtcgcgc atgcttttca acatcatctg cacgaacatc
    31501 ccttccacct ggcgggccac cggacggata tttgccgctg gatcttcgcc cgctttcgcc
    31561 tttagttcgt tgagtgattg cgcatcccag gccgcacttg ccagtagttt gctgtcgctg
    31621 atcatcagat gatttccagt tttgcccgca gacatcccgc actttgcatt gattgcagta
    31681 tggacatcag atccatcggc gtagcgccca gcgcattgag cgcacgcacc acgttattga
    31741 ggctggcgct ggaacgtacg ctttgcagcg aaccgccgct ctggcgtaaa tcgatctgcg
    31801 tttgtggagt aaccacagtc tgcccaccac caaacggtgt atctggctgg ctgacattgg
    31861 cctgacgatt aactgttact gagagattcc cctgcgctac cgcgcagctg tcgagggtca
    31921 cttcgcgatt catcaccacc gaaccagtgc gcgagttaat cactacttta gcgtcctgcg
    31981 gggtgacatt aacctgcata ttctggatat cggcaaggaa acggacctgg gaactgttgc
    32041 cacttggtac gcgcacctga atagtccgcg catctaacgc ggtggcgctg ccatatccac
    32101 gcacgcggtt gatggtgtca gcgatttgct gcgccatact gaaatcttcg tcgttaagtt
    32161 gcaaattaag ggtattcccg acgccaaact ggctgggcaa ttcacgttca ataaccgcac
    32221 cattggtgat ccgtccaccg ttcagttgat tcacctgaac actgctaccg ccagcggagg
    32281 cccctgcgcc gccaaccaga atattgccct gcgccagcgc atacacctga ctgtcaacgc
    32341 ccttaagcgg tgtcatcaat aacgtaccgc cacgcagact tttggcattt cccatggaag
    32401 aaaccaccac gtcaatggtt tgcccctgac gtccaaacgg tggaagtgac gctgtcacca
    32461 taaccgcagc gacgtttttt agctgcatat tagtgcccgt cggaacggta attcccagct
    32521 gtgagagcat gttattaagc gtttgtgtgg taaacggcgt ctgagttgtc tggtcacctg
    32581 taccatcaag ccccaccacc aggccatagc caatcagtga gttttgcctt accccctgaa
    32641 cactggtgag atcgcgaata cgctcagcct gagccgccgt ggtaaccagt aaaagaatta
    32701 atgcagagag aaatttaatc accacagcct cacttacatt ggcgacaggt taaggaagaa
    32761 acgctgcaac cagcccatat tttgcgcttc gttaatgtag ccattgccta cgtattcaat
    32821 gcgcgcatct gccacctgag tagacggtac ggtattgctg ccgctgatag tgcgtggatt
    32881 aaccacccca gagaagcgaa taaattcggt accctgatta atggcgatct gtttttcacc
    32941 caccacatgc aggttgccgt tgaccagtac ctggtcaacc gtcaccgtca acgtaccgct
    33001 aaaggtattg ctggcattgg ccccgccttt tccgttgaac gtgttaccac cggaggcttc
    33061 gacatcggca cgagcgttac caaacaaccc ctgcaaatag cgcggcacag tatcaaagcc
    33121 aaaattagtt ttaccgtcac ggctggcatt cgcagaggag cttttgctgg cgctgacgtt
    33181 ctcctgcaac acgatagtca gcgtatcgcc aatattgcgt ggtcgacgat cttcaaacag
    33241 cggttgatag ccatagttaa tcggctgagc agactggaaa atagacccgt tggcgacggg
    33301 cgtcggaccg ggaaccggtt gtgcactggt cgccccctgt accagcggcg tggagggtat
    33361 ccaggcgcag ccggttagtg aaagcgccaa caagctggaa atggcataag tatgcgcagc
    33421 gtttttttgc atggctatca tcttcaaaaa tcagtaaacc ggtgaacctg cccaccggtt
    33481 aagccttaga gttgcgtcag tttttgcagc atctgatcgg tggtggacac cgctttactg
    33541 ttgatttcgt aagcgcgttg cacctgaatc atattgacca gttcttccgc cacgttgacg
    33601 ttagacgttt caacataccc ttgatacagc agtcccgcgc cgttcaggcc cggcgtgctt
    33661 tcgttcggtg caccggagga ttgcgtttcg gtgtagaggt tttcgccaat gctctccagt
    33721 ccggtgtcat tcataaaggt ggtgagattg agctgcccaa cctgaaccgg agctgcctgg
    33781 ccttgttggg ttacgctgac cacgccatca cgaccgatgg tgatacttaa cgcattcgcc
    33841 ggaatggtga tcgctggctg cacctgaaaa ccaccggccg tcaccagctg cccgttctga
    33901 tccacctgga aagagccgtc acgggtatag gctgatgagc catctggcaa catcacctgg
    33961 aaaaagccct gccctttaat cgcgacgtct ttactgttat tggtttgcga caggttgccc
    34021 tggctgtgta aacgttccgt ggcgaccggg cgtacccccg taccgatttg taatccggat
    34081 ggtaaggtgg tttgttcgga agactgtgcc cccggctggc gaatggtttg ataaagcaga
    34141 tcttcaaaca ccgcgcgctg acgcttaaaa ccgttggtac taacgtttgc caggttgttg
    34201 gcaatgacgt ccatattggt ttgctgggcg tcaaggcccg ttttggcgat ccataatgaa
    34261 ctgatcataa aatgtcctgt agtggattag tgacaaagtg catatgtctt cttgccggat
    34321 gcggcgtaaa cgccttatcc ggcctacaag agctcgcaaa atcgataaat tgcgattgcc
    34381 cccatagacc cgataaactt gcgcataggg caacttatac ttgtcatgta tcctttcaat
    34441 taactcatcg acagcagttg gttggcacgg cctgcgttat catcgacgct gctgatcacc
    34501 ttcatctgca tttcaaaacg ccgtgcgctg gcaatcatgt cgctcattgc cgcaacggca
    34561 ttgacgttac tgccttccag aacccccgac atcacacgca aggttggatc tgcctgcagt
    34621 accggcccac gcgtagcctg gctttctgcg cttaaacgaa aaatgccatc gtcaccgcgc
    34681 tggacttcgc tgcccgtggc tttcaccagc ttcagacgcc ccactggcgc aaccgtattt
    34741 gccggatcgc ccggattcag cgccgagatt gtgccatcgg cagcaatagt gatttccgcc
    34801 ccttccggca cagcaattgg ccccgcctcg cctaccaccg gatgtccctg aattgtcagt
    34861 tgcccggtgg gatcaacctg aatgctgcca ttacgcgtat acccttcact gccgtcagcg
    34921 gtctgcacga ccagccaacc atcctgctgc aacgcaacgt ccagcgggcg cgaggtgtaa
    34981 tccatcttgc cgggcgtcat gtctgcaccc ggtgttgacg ccgttaccag cgtgcgcgtg
    35041 ggcagtgaaa gcccttccac cggcactgcg cgcaaggcgt ttaactgcgc gcgaaaaccg
    35101 ggcgtagagg cattggccag attactggcg gttaccgcct gttgattcag cgtctggctg
    35161 gctgctccca tcgcggtata aattgcgtga tccattgagc tatcccgtca gcgattagcg
    35221 taagttaacc agcgtattga ggatctggtc ctgggttttg atggtctggg cgttagactg
    35281 atagttacgc tgggcaacga tcatattgac cagttcttta ctgagatcga cgttggacgc
    35341 ttccagcgca ccgttggtca gggtgccaaa gttgcccgtc ccggctgtcc ccaacagcgc
    35401 cacgccagaa gattgcgtag cggaccagac gttgtcgcct tcggatgcca gaccttcgtt
    35461 gttggcaaag ttcgccagta caatctgccc cagcagttgg gtttgttcgt tggaatagtt
    35521 gccgacaacc gtaccgtcat cattgatttg ataactcacc agatcgcccg gtttgtagcc
    35581 gttctgggtg gttgccacaa tgttgttagc gccggtattt tgctgcatgg agttgaggaa
    35641 gctcaggcta aacgtggcgg gatctgcgcc gttaattgcg ccggtggtaa tatcctttgt
    35701 tggatctgag gtcagaacgc cattggcatt aaacaccagc gtcgttgcag ggttagctgt
    35761 gcctgttgga tcactgctat ccagggtgta aacatcccag ttattatccc cggtcttcac
    35821 aaagtagacg ctcatgtcat gagcattacc ctgactatcg aaaacagtca ccgaaccttt
    35881 tttgttatag ctatccgcat tgctggcatc aaatgcgtta acagagggaa gcgaatcact
    35941 ggaattcagg ttgatctgca tcgacgccgt ggtggtagtt ttcgctgcca tcagggtatt
    36001 cggaatcgaa atattggtcg gattcgcccc ttgctgaata gtcggcggcg taccggttgc
    36061 cgggtaaccc gtcagctgta aaccttgcat attcaccagg ttacggtttt catccagctt
    36121 aaattgtccg ttacggctgt agaataccga accattgctg tctaccagac ggaaaaaacc
    36181 gttctggctg atagcaacgt ccagaccacg cccggtgttg gtggtcgtgc catcggtaaa
    36241 gtcctgagtg ataccagcaa cttttacccc cagtcccact ttcgaaccgg caaacatatc
    36301 ggcaaaagag gccgtgcctg atttaaagcc gtaggtggcg gagttggcga tattgttgcc
    36361 aataacatcg aggttggtgg cggcagcgtt taatccgcta accgcttgag aaaaggccat
    36421 gactgactcc tgaaatgtga aggcttaaat tatctgccgt acttcgtcga gggtagtggt
    36481 gccgtaagtg ccaagatcca gcgtattacc gttgttgccg cggatcaccc cctgcaccag
    36541 agcaaactgc agcggttggg caaccagttg tgtaccaccg ttactggcgc taatcgctac
    36601 attgtaagaa ccgttcggcg cagtgctgcc gtcagtcaac gtaccgtccc aggtgaaact
    36661 gtggactccg gcagtcagtt caccgatatc aatggtacgg acgaccgcgc cgtttttatc
    36721 ggtgatggtg gccgtcactt tgtctgctgc ctgttgcaac tcaacaccaa acggcgtggt
    36781 cgtggtcaca gccccttctt cactgccggt tcccgcaaga actgtggtac cggggatcat
    36841 cacgccgtga ccgataaggt tactggcctg taacgactga ctgttatcaa tctgtccgga
    36901 aatagatccg agcgtggtat tgagtttttc aatcccactg accgtgctga tttgtgccaa
    36961 ttgcgacgtc agctcgttgt tttccattgg attggtcggg tcctggtttt tcagctgcgc
    37021 caccaacaaa gtcagaaaac tgctttgtaa atctgcggcg ttgctgcccg tgagcgaact
    37081 actgctggtg gtactgacgc cggtattcgt cggatcggtg gtggttaccg caatagacat
    37141 agctttctcc tttattgacc gagcgtaagg gttttcagca tcatgctttt caccgtgttg
    37201 agcacttcaa cattggcctg ataactgcgt gacgccgaca tggtgttaac catctccccg
    37261 acgacatcaa cgtttggcat tttcacgtag ccctttgcat ccgccagcgg attacccggt
    37321 tcataaacca gtttgtccgg ggcctgactt tctataacat cggcaacctt tacgccgcct
    37381 gtcgcagcac ctggtgcagc gttaacctgg aataccacct gttttgcccg atatggctgt
    37441 ccatcgggac cggtcacgct atcagcattc gccagattac tggccgccac gttcagacgc
    37501 tgggactggg cagttaacgc cgacccggcg atatcaaaaa tattcagcag tgccatccgt
    37561 taatttccgc tctgtaaaac gttcatcatg cctttaattt gcccgctcaa cgcgctaagg
    37621 ctcatctggt attgcaggct gttatcggca aactgggtgc gttcgcgatc catatcgaca
    37681 gtattaccgt caagcgaagg ctggtccgga atacggtatt gcagttctgc ggaaggaggc
    37741 gtcagcgcct gcgccggaat gtgttgcgtt gaggtcatcg tcagagcaac cacactggtt
    37801 gcatcccgtc cacgttgcat gactttttta agttcactgg caaaatcgat atcgcgcgcc
    37861 tgataaccag gggtatcggc attggcgatg tttgctgcca gcacttcctg acgctgggcg
    37921 cgcagattga gcgcctcttg ttgaaaacgt aaggcggcgt cgagcttatc gagcatatat
    37981 cctccgcagg tatcaaaatt ctgccatcag cttaaatgcc tttacgcgcg cgttatcggc
    38041 ggaataaacg caaaatgggt cgctatttat gccgttgatg gtcattgcgg acaggtacaa
    38101 ttcacgttgt agaaatggct gggggcgaaa atgctgacaa taaaacgtag cgtggcgatc
    38161 atcgcgatac tcttcagtcc gttaagtacg gcgagcaatc tcacatcgca attgcacaac
    38221 ttttttagcg cccaactcgc gggggtaagt gatgaggttc gtgtttctat tcgtacagcg
    38281 cccaatctgc taccgccgtg cgagcagcca ttgctttcga tgagcaataa ttcccgcctg
    38341 tggggcaatg tgaatgtgtt ggcacgctgc ggtaacgaca aacgatattt acaggttaat
    38401 gtacaggcca caggaaatta tgtggttgcc gcgatgccca ttgtgcgtgg aggaaagctg
    38461 gaagctggca atgtcaaact gaaacgcgga cggctggata ccttgccacc gcgtacggtg
    38521 ctggatatca atcaacttgt tgatgccgtt agcctgcgcg atctatcacc cgatcaacct
    38581 atccagttaa cccactttcg ccaggcatgg cgggtaaaag cgggacaacg cgtcaacgtt
    38641 atcgccagcg gtgatgggtt tagcgccaac gcagaaggtc aggcgctgaa caatgcagcc
    38701 gtcgcacaga atgcgcgggt gcgcatgata tcgggacaag tggtcagcgg cgttgttgat
    38761 gcagatggga atattcttat aaacctgtaa gctgttaaag attacccgtc ccttgccgat
    38821 aaataagcaa cacatgataa aagcgccctc aatgaggaat aaaccatgag tattgatcgc
    38881 acttcgcctc tgaagccagt aagcaccgtt caaccgcgcg aaaccactga cgcgccggta
    38941 acaaacaccc gagcggcaaa aacaacagcc tccaccagca ccagtgtgac gttaagcgac
    39001 gcgcaagcaa aactgatgca acccggcagc agtgatatca atcttgaacg tgtcgaagcg
    39061 ttaaagctgg cgattcgtaa cggtgaacta aaaatggaca ctggcaaaat tgccgatgcg
    39121 ctgatcaacg aagcgcagca agacttgcag agtaattgat cgtatgacgc gtcttgcaga
    39181 gatcctcgac cagatgtccg ctgtgcttaa cgatctcaaa acggtaatgg atcaagagca
    39241 gcaacatctc tctatggggc agatcaacgg cagccagttg caatggatta cagaacaaaa
    39301 aagctcactg ctggcgacgc tggattacct cgaacagtta cgcaggaaag aacccaatac
    39361 agcaaatagc gttgatatta gtcaacgctg gcaggaaatt gctgtgaaaa cgcagcaact
    39421 acgccaaatg aatcaacata acggctggtt actggaagga cagattgagc gcaatcaaca
    39481 ggcgctggaa atgttgaaac cgcatcagga accgacgctg tatggggcga acggtcagac
    39541 ctcaaccccc tatcgcggca gtaaaaagat ttcgatctga agattatctc cggcctgcac
    39601 tgcaggccgg aatgtatcgt tacaccgtcc ggcgggcaaa ttctttaact ttgaagccca
    39661 gtactgccag tgcagcgaag tacgcggcaa tccccgccag cacgaccgcc attaaacgca
    39721 gtaaacgcca gggcatggta cccaatgacc actccggcat gatatgtaac atacctaaaa
    39781 gcacgccaga catcaccagt accgccacca ccagacgcaa cagaaacgcc atccagccgg
    39841 gttgcggagt aaagattttc tgcttacgca actgccagta aagcagcgaa gcattcagac
    39901 acgccgccag accaatagaa agtgacagcc cggcatgttt caacggacca ataaacgcca
    39961 ggttcatcaa ttgcgttaaa atcagcgtaa cgatggcaat tttcactggc gttttaatgt
    40021 cctggcggga ataaaagcca ggagccaaca ctttcactac aatcaggcca atcaaaccca
    40081 ccgagtaggc aattaacgcc cgctgggtca tcagcgcatc aaaggcggta aatttaccgt
    40141 actggaacag cgaaacggtt aacggaccgg aaagaatgcc caacgcaacc gcacttggca
    40201 gcgccaacag gaaacaaaga cgcagccccc agtccatcaa acggttgtat tcatcatgat
    40261 tgccactggc aaaacttttc gacagcgacg gtagcaaaat ggtgccaagc gccacgccca
    40321 gcacaccgga cggaaactcc attaagcggt cggcgtaata catccacgac accgaaccgg
    40381 aagcaagaaa cgaggcaaaa atggtgttga tgattaagga gatctggctc acagagacgc
    40441 caaggattgc cggtcccatc tgtttcacca cacgcattgc tccggcatcg tggaagttaa
    40501 tgcgtggcaa aaccagcatg ccgatcttct tcagatgagg tagctgatac accagctgca
    40561 ggatgccgcc gaccgtaaca gcccaggcca gcgccagcac cggtggatta aagtacggcg
    40621 cggcaaacag cgcaaaacca atcatgctga tattgagcaa tgtcggggca aacgccggaa
    40681 ttgagaagcg gttccaggta ttcagaatcg ctcccaccag cgacgccagg gagatcagca
    40741 agatataggg aaaggttatc tttagtagct ggctggtcag ggcaaatttg tcggcagtgt
    40801 cagcgaagcc tggcgcggtc accatgatca cccacggtgc agcgagcatg ccagcgaccg
    40861 tcacaaccgc cagcgcaagt gtcagcaggc cagaaacata agagacaaag acccgcgtgg
    40921 cgtcttcacc ctgcttgctt ttatattctg ccagaatcgg tacaaatgcc tgggaaaatg
    40981 ccccttcggc aaagatacgg cgtaacaagt taggaagttt aaaagcgacg aaaaaggcgt
    41041 cggttgccat ccctgcgcca aagattctgg cgacaattgc gtctcgtgcg aagccaagca
    41101 cacgcgaaaa catggtcatc gagctgacgg cggccagcga ttttaataaa ttcatcggtg
    41161 ttctaatcca gacccacggg caaaacgcct gcaaggcagg cgctgaaaga ggcgaatagt
    41221 ctacctggat tatggtgaat tgctaccgcc agatgttaca gggtttattc actcatcgca
    41281 tcgcgccaga tcttgtcaac gatacgttgc gccagcacgg cctgttcgcc ggctgtttgc
    41341 ggaactgtct gattttgcac gcattcaatg aagtgccgcg cacagtcgac aaatccacgt
    41401 tgctcaagcg tactctgcca accaggaatc ggtttatgca ctacgccctg cccgcgctcc
    41461 tcacgccatt cgcgcatatc cgtaatgtcg atcagcgcac catcagtcac ggcctgcacg
    41521 gtttcacgct gacttccggc ccggcgatgc atacaggtgg tgatctgcaa aggaccagcc
    41581 gaaaaatggt gctcggcaaa cagcatttcg ccggactcat tggtcagcaa cgtaccgctt
    41641 tcgagagttg cattgccgcc agacaaccac agcgcggtat caaccacatg caaataatca
    41701 tccagcaatg tgaaataaag atcgtgtggc ccgacgctat tggtacgatg tttatccatt
    41761 cttagcgagg acgcggtggc gagttgcgtt tttaactcac cgtagagtgg tgcgaaacga
    41821 cggttaaaac cgaccatcaa cgtcagtttt ttccgcgccg ccagttccac cagccgttca
    41881 gcatcgcgca gattttctgc cagcggttta tcgacacaga catgtacccc cgcattgagt
    41941 aacgtactga ccacgccaaa gtggctggcg gtgctggaat gcacaaaaac cgcatcgcaa
    42001 ctggcggcaa ggctggataa cgaatcagca taaggaatgc gccagctgtc acaaattggc
    42061 agggctttgg cgcgcgtagg cgaccaggca ccttgtaacg tccagtcaga cgctgccgcc
    42121 agcaccggta accacgcttt ttgcgcgatg ccgcctaacc ccactacgcc gatacgtaat
    42181 tttttcactt taatctccca gatgggccag caacgaatca agacgctgtt tcagttctgc
    42241 cacttcgatt tccagggctt cgacgcgggc ctgtaaatca ccgtcaaccg cgttcgacat
    42301 atccgttacc gccggcggat tctcaacctc accactgaaa agatgcatgt agcggctttc
    42361 gcgtttaccc ggttcgcggg ccagacgcac cacaaaagga ccatcttcgc gattcgccag
    42421 ttgttccagc gtcgactcca cttccgccat atcgctgaat tcatacattc gcgcggcgcg
    42481 gctgcgcagt tcacccggcg tctgggcacc acgcaataac agcgtggtga ttaacgccac
    42541 ttctgcggcg ctcagtttca gatcgccaaa ttctgaatta caaaaacgtt gctcatattt
    42601 ggtgacccga ttaccaaaac cgctcaccgt gcgtagataa tgacgtttga ccagattatc
    42661 cagctgttcc tgcacttcag attcactcag attcataacc ggttcacggt tcgttttctg
    42721 attacaggcc gtgactacac catttacgga gagcgggtat tgctccggcg tggtcacctg
    42781 tttttccagc aagcaaccaa tcacgcgggc ttccagggcg gtcagttgat atttcatgag
    42841 attctcctta gcggccgggc gtccagtctg gggtagttaa tgccgtcagt acgtgatcgc
    42901 gccattgtcc atcaatcaac agatagtctt tcgcatagcc ttctttttca aaacccagtc
    42961 gcgccagtaa atcaccgctg cgtttattgt gcggcatata attagccata atgcgatgaa
    43021 tatgttgggt gcgctgcata taacgaatgg ctgcgctcag agcttcaaac atgagtcctt
    43081 tgccctgcca tttttgccca atcgaataac cgagatagca ggcatgaaaa gagccgcgaa
    43141 caacattgga aaaattggca acgccaataa tctctttttc gtccgggtcg aataagccaa
    43201 agtagaaagc tgaaccttgt ttatgaaatt cgttaatcat ccctagcctg gcctgccagc
    43261 ctgatggata acagtggctt tcgtcgcgca ctggctccca gggcttgagg aaatggcgat
    43321 tctctgcgta ataatccgcc agacgccagg catcacgatc atgcaccaga cgcacgacca
    43381 gtcggtctgt ggttaagcgc acttttggca cgttactgcg atagccaaac attctatacc
    43441 tactccttcc cgtaacgtct ttaatacgct gtaagtatta ctatacctgc gcaaccagac
    43501 cctgtgaaaa gtccagaatg ccattttctg cattattcac atatttgata aaaactctct
    43561 atcaaagcga cgttttgata aaaaaatatt gtccccgaag tgatgggaaa agtcttagcg
    43621 gcaatgcaga atagaaagtg ctcatctttt atttcccggg aggggaaatg tcccgcgtgt
    43681 cgcaggcgag gaacctgggt aaatatttcc tgctcatcga taatatgctg gtcgtgctgg
    43741 ggttctttgt tgtcttcccg ctgatctcta tccgcttcgt tgatcaaatg ggctgggccg
    43801 ccgtcatggt cggtattgcc ctcggtctac gccagtttat tcagcaaggt ctgggtattt
    43861 tcggcggcgc aattgccgac cgctttggtg ccaaaccgat gattgtcacc ggtatgctga
    43921 tgcgtgccgc cggattcgcc acaatgggta tcgcccacga accgtggcta ctgtggtttt
    43981 catgcctgct ttcgggactc ggcggcacgt tgtttgatcc tccgcgttcg gcgctggtgg
    44041 tgaaactaat ccgtccacaa cagcgtggtc gttttttctc gctgttgatg atgcaggaca
    44101 gtgccggtgc ggtcattggc gcattgttgg ggagctggct gttgcaatac gactttcgcc
    44161 tggtctgcgc cacaggggca gttttgtttg tgctgtgtgc ggcgttcaat gcgtggttgt
    44221 taccagcatg gaaactctcc accgtacgca cgcccgttcg cgaaggcatg acccgcgtga
    44281 tgcgcgacaa gcgttttgtc acctatgtcc tgacgctggc gggttactac atgctggctg
    44341 tacaagtgat gctgatgctg ccaattatgg tcaacgacgt ggctggcgcg ccctctgccg
    44401 ttaaatggat gtatgccatt gaagcgtgtc tgtcgttaac gttgctctac cctatcgccc
    44461 gctggagtga aaagcatttt cgtctggaac accggttaat ggctgggctg ttgataatgt
    44521 cattaagcat gatgccagtg ggcatggtca gcggcctgca acaacttttc accctgattt
    44581 gtctgtttta tatcgggtcg atcattgccg agcctgcgcg tgaaacctta agtgcttcgc
    44641 tggcagacgc aagagctcgc ggcagctata tgggatttag ccgtctgggt ctggcgattg
    44701 gcggcgctat tggttatatc ggtggcggct ggctgtttga cctgggcaaa tcggcgcacc
    44761 agccagagct tccgtggatg atgctgggca ttattggcat cttcactttc cttgcgctgg
    44821 gttggcagtt tagccagaaa cgcgccgcgc gtcgtttgct tgaacgcgac gcctgatttg
    44881 atactccccc ctgactcctc catactgaaa gagaactgac agccaaatgg aggagtcacg
    44941 tgaagctata catttacgat cactgccctt actgcctcaa agcccgcatg attttcggcc
    45001 tgaaaaatat ccccgtcgaa ttacatgttc tgctcaacga cgacgcagaa acacccaccc
    45061 ggatggtcgg tcaaaaacag gttcccattc tgcaaaaaga tgacagccgc tatatgccag
    45121 aaagcatgga catcgttcac tatgtcgata aactcgacgg caaaccgtta ctgaccggca
    45181 aacgttcccc tgccattgaa gagtggctgc gcaaggtcaa tggctacgcc aacaaactgc
    45241 tgttgccgcg ttttgccaaa tcggcatttg atgagttttc tactcctgcc gcgcgcaaat
    45301 atttcgtcga caagaaagag gccagcgcgg gtaattttgc cgacctgctg gcccactctg
    45361 acggtctgat taagaatatc agcgatgatt tacgtgcgct ggacaaactg atcgtcaaac
    45421 cgaacgccgt gaatggcgaa ctttcggaag atgatattca gctatttccg ctcctgcgta
    45481 atctgacgct agtagccgga atcaactggc caagccgcgt tgctgattac cgcgataata
    45541 tggcgaaaca gacacaaatc aatttgttat catcaatggc gatttaatcc tgacccgtcg
    45601 cggcagcctg tattcgcggg ttgccgccgt tttcagtttt ttctgctggc gcgtttatcg
    45661 ctggtgacac atgctgcgtt gtgtcaggat attgacgcag atttcatgtc gaggactacc
    45721 atgaacaagt ttttatttgc cgctgcattg atcgtcagtg gcctgctcgt tggctgtaat
    45781 caactcaccc aatacaccat caccgaacaa gaaattaacc agtcgcttgc gaaacataat
    45841 aatttctcaa aagatatcgg tttacccggc gtggctgacg cccatatcgt tctgacaaac
    45901 ctgaccagcc aaattggtcg cgaagagccg aataaggtta ccctaaccgg agacgccaat
    45961 ctggacatga actccctgtt cggtagccag aaagcgacca tgaaactgaa gctgaaagcg
    46021 ctgcccgtgt ttgataaaga aaaaggcgcg atcttcctga aagagatgga agtggtcgat
    46081 gcgacggtac aaccggaaaa aatgcaaacg gtgatgcaaa cgttgcttcc ctatttaaac
    46141 caggcattac gcaattactt taaccagcaa cctgcttacg tcctgcgcga agatggcagc
    46201 cagggcgaag caatggcgaa gaaactggcg aaaggcattg aagtgaagcc aggcgaaatt
    46261 gtcattccgt ttactgatta atcacgaggg cgcattcgcg ccctttattt ttcgtgcaaa
    46321 ggaaaacgtt tccgcttatc ctttgtgtcc ggcaaaaaca tcccttcagc cggagcatag
    46381 agattaatga ctgcaccatc ccaggtttta aagatccgcc gcccagacga ctggcacctt
    46441 cacctccgcg atggcgacat gttaaaaact gtcgtgccgt ataccagcga aatttatgga
    46501 cgggctatcg taatgcccaa tctggctccg cccgtgacca ccgtagaggc tgccgtggcg
    46561 tatcgccagc gtattcttga cgccgtacct gccgggcacg atttcacccc gctgatgacc
    46621 tgttatttaa cagattcgct ggatcctaat gagctggagc gcggatttaa cgaaggcgtg
    46681 ttcaccgctg caaaacttta tccggcaaat gcaaccacta actccagcca cggcgttacg
    46741 tcagttgacg caatcatgcc ggtacttgag cgcatggaaa aaatcggtat gccgctactg
    46801 gtgcatggtg aagtaacaca tgcagatatc gacatttttg atcgtgaagc gcgctttata
    46861 gaaagcgtga tggaaccgct gcgccagcgc ctgaccgcgc tgaaagtcgt ttttgagcat
    46921 atcaccacca aagacgctgc cgactatgtc cgtgacggaa atgaacggct ggctgccacc
    46981 atcactccgc agcatctgat gtttaaccgc aaccatatgc tggttggtgg tgtgcgtccg
    47041 cacctgtatt gtctacccat cctcaaacgc aatatccacc aacaggcatt gcgtgaactg
    47101 gtcgccagcg gttttaatcg tgtattcctc ggaacggatt ctgcgccaca tgcacgtcat
    47161 cgcaaagaga gcagctgcgg ctgcgcgggc tgtttcaacg ccccaaccgc gctgggcagt
    47221 tacgctaccg tctttgaaga gatgaatgct ttgcagcact ttgaagcatt ctgttctgta
    47281 aacggcccgc agttctatgg cttgccggtc aacgacacat tcatcgaact ggtacgtgaa
    47341 gagcaacagg ttgctgaaag catcgcactg actgatgaca cgctggtgcc attcctcgcc
    47401 ggagaaacgg tacgctggtc cgttaaataa taaaaatcgt agcttcctgt tgtcattagg
    47461 ttattttacc tgtataaata accagtatat tcaacagggg gctattatgc gaattgaagt
    47521 caccatagcg aaaacttctc cattgccagc tggggctatt gacgccctgg ctggcgaact
    47581 ttcccgccgt attcagtatg cgtttcctga taatgaaggc cacgtatcgg tacgttatgc
    47641 cgcagcaaat aatttatcgg ttattggcgc gacaaaagaa gataaacagc gcattagcga
    47701 aattctccag gagacgtggg aaagcgccga tgactggttt gtcagcgaat aatatgcagt
    47761 gatttt
//
LOCUS       DAHHTG010000020        47662 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.020, whole genome shotgun sequence.
ACCESSION   DAHHTG010000020 DAHHTG010000000
VERSION     DAHHTG010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 47662)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47662)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47662
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.020"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            complement(30..203)
                     /locus_tag="NBI74_001142"
     CDS             complement(30..203)
                     /locus_tag="NBI74_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1931509.1"
                     /translation="MSEFDAQRVAERIDIVLDILVAGDYHSAIHNLEILKAELLRQVA
                     ESTPDIPKAPWEI"
     gene            complement(351..2336)
                     /gene="pdeR"
                     /locus_tag="NBI74_001143"
     CDS             complement(351..2336)
                     /gene="pdeR"
                     /locus_tag="NBI74_001143"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
                     /protein_id="HCD1931510.1"
                     /translation="MKTVRESTTLYNFLGSHNPYWRLTESSDVLRFSTTEITEPDRIL
                     QLSAEQAARIREMTVITSSLMMSLTVDESDLSVHLVGRKINKREWGGNASAWHDTPAV
                     ARDLSHGLSFAEQVVSEAHSAIVILDSRGNIQRFNRLCEDYTGLKEHDVIGQSVFKLF
                     MSRREAAASRRNNRVFFRSGNAYEVELWIPTRKGQRLFLFRNKFVHSGSGKNEIFLIC
                     SGTDITEERRAQERLRILANTDSITGLPNRNAMQELIDHAINQADNNKVGVVYLDLDN
                     FKKVNDAYGHLFGDQLLRDVSLAILSCLEHDQVLARPGGDEFLVLASNTSQSALEAMA
                     SRILTRLRLPFRIGLIEVYTSCSVGISLSPEHGSDSAAIIRHADTAMYTAKEGGRGQF
                     CVFTPEMNQRVFEYLWLDTNLRKALENDQLVIHYQPKITWRGEVRSLEALVRWQSPER
                     GLIPPLDFISYAEESGLIVPLGRWVILDVVRQVAKWRDKGINLRVAVNISARQLADQT
                     IFTALKQVLQELNFEYCPIDVELTESCLIENDELALSVIQQFSRLGAQVHLDDFGTGY
                     SSLSQLARFPIDAIKLDQVFVRDIHKQPVSQSLVRAIVAVAQALNLQVIAEGVESAKE
                     DAFLTKNGINERQGFLFAKPMPAVAFERWYKRYLKRA"
     gene            2390..2493
                     /locus_tag="NBI74_001144"
                     /pseudo
     CDS             2390..2493
                     /locus_tag="NBI74_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791239.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2570..4504)
                     /gene="rnb"
                     /locus_tag="NBI74_001145"
     CDS             complement(2570..4504)
                     /gene="rnb"
                     /locus_tag="NBI74_001145"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exoribonuclease II"
                     /protein_id="HCD1931511.1"
                     /translation="MFQDNPLLAQLKQQLHSQTPRAEGVVKATEKGFGFLEVDAQKSY
                     FIPPPQMKKVMHGDRIIAVIHSEKERESAEPEELVEPFLTRFVGKVQGKNDRLAIVPD
                     HPLLKDAIPCRAARGLNHEFKEGDWAVAEMRRHPLKGDRSFYAELTQYITFGDDHFVP
                     WWVTLARHNLEKEAPDGVATEMLDEGLVREDLTALDFVTIDSASTEDMDDALFAKALP
                     DGKLQLIVAIADPTAWIAEGSKLDKAAKIRAFTNYLPGFNIPMLPRELSDDLCSLRAN
                     EVRPVLACRMTLSTDGTIEDNIEFFAATIESKAKLVYDQVSDWLENTGDWQPESEAIA
                     EQVRLLAQICQRRGEWRHNHALVFKDRPDYRFILGEKGEVLDIVAEPRRIANRIVEEA
                     MIAANICAARVLRDKLGFGIYNVHMGFDPANADALAALLKTHGLHVDAEEVLTLDGFC
                     KLRRELDAQPTGFLDSRIRRFQSFAEISTEPGPHFGLGLEAYATWTSPIRKYGDMINH
                     RLLKAVIKGETATRPQDEITVQMAERRRLNRMAERDVGDWLYARFLKDKAGTDTRFAA
                     EIVDISRGGMRVRLVDNGAIAFIPAPFLHAVRDELVCSQENGTVQIKGETAYKVTDVI
                     DVTIAEVRMETRSIIARPVA"
     gene            complement(4572..5699)
                     /locus_tag="NBI74_001146"
     CDS             complement(4572..5699)
                     /locus_tag="NBI74_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707196.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CMD domain-containing protein"
                     /protein_id="HCD1931512.1"
                     /translation="MEQRHITGKSHWYHETQSSTAEYDVLPLVPEAAKVSDPFLLDVI
                     LDEETLAPFLSWLVPARVLAVELFPDPLTVTRSQTFTAYERLSTALTVAQVCGVQRLC
                     NYYSARLTPLPGPDSSRESNHRLAQITQYARQLASSPSIIDNRSRQHLNDVGLTAWDC
                     VIINQIIGFIGFQARTIATFQAYLGHPVRWLPGLEIQNYADASLFADESIRWRNSYEV
                     EKLPEEHTKSSTAELCQLANTHSLHPISLSLLEKLLNSTRVNTQPDNQLAALLCARIN
                     GSPACFAACMNSSNEYKKISPLLRKGENEINRWADRHSVERATVQAIQWLTRAPDRFS
                     AAQFSPLLEHEKSSTQIINLLVWSGLCGWINRLKIALGETY"
     gene            complement(5844..6632)
                     /gene="fabI"
                     /locus_tag="NBI74_001147"
     CDS             complement(5844..6632)
                     /gene="fabI"
                     /locus_tag="NBI74_001147"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HCD1931513.1"
                     /translation="MGFLSGKRILVTGVASKLSIAYGIAQAMHREGAELAFTYQNDKL
                     KGRVEEFAAQLGSDIVLQCDVAEDTSIDTMFAELGKVWPKFDGFVHSIGFAPGDQLDG
                     DYVNAVTREGFKIAHDISSYSFVAMAKACRSMLNPGSALLTLSYLGAERAIPNYNVMG
                     LAKASLEANVRYMANAMGPEGVRVNAISAGPIRTLAASGIKDFRKMLAHCEAVTPIRR
                     TVTIEDVGNSAAFLCSDLSAGISGEVVHVDGGFSIAAMNELELK"
     gene            7110..7676
                     /locus_tag="NBI74_001148"
     CDS             7110..7676
                     /locus_tag="NBI74_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCD1931514.1"
                     /translation="MTSKLEIRHKQRQDEIINAARRCFRRCGFHAASMSQIASEAQLS
                     VGQIYRYFANKDAIIEEMVRRIIDFRIAQMDIDARTDHFPEVLALRKSLNEDDDALML
                     EVAAEATRNPRVMAMLEEADARMFANGCAHMKRMHPHLSDEHIRCCVEVFATMMEGTV
                     YRRLTPQKSDPQHLQEIYQDIVSMLINK"
     gene            7825..8946
                     /locus_tag="NBI74_001149"
     CDS             7825..8946
                     /locus_tag="NBI74_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HCD1931515.1"
                     /translation="MKYIATSVVAMLLLSGCDNTQSNNSSPSETEVGVVTLKSQPVSV
                     VSELTGRTSAALSAEVRPQVGGIIQKRLFKEGDLVKAGQPLYQIDAASYQAAWNEARA
                     ALQQAQALVKADCQKAQRYARLVKENGVSQQDADDAQSTCAQDKASVEAKKAALETAR
                     INLDWTTVTAPISGRIGISSVTPGALVTASQDTALTTIRGLDTMYVDLTRSSVDLLRL
                     RKQSLATNSDTMSVSLILEDGTTYSEKGRLELTEVAVDESTGSVTLRAIFPNPQQQLL
                     PGMFVRARVDEGVMENAILAPQQGVTRDAKGNATALVVNKDNKVEQRTLETGETYGDK
                     WLVLNGLHNGDRLIVEGSAKVTSGQTVKAVEVQANGGNA"
     gene            8946..12053
                     /locus_tag="NBI74_001150"
     CDS             8946..12053
                     /locus_tag="NBI74_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit"
                     /protein_id="HCD1931516.1"
                     /translation="MFSRFFVRRPVFAWVIAILIMLAGILAIRTLPVAQYPDVAPPTI
                     KISATYTGASAETLENSVTQVIEQQLTGLDNLLYFSSTSSSDGSVSINVTFEQGTDPD
                     TAQVQVQNKIQQAESRLPSEVQQTGVTVEKSQSNFLLIAAVYDTTDKASSSDIADWLV
                     SNVQDPLARVEGVGSLQVFGAEYAMRIWLDPAKLASYSLMPSDVQSAIEAQNVQVTAG
                     KIGALPSPDTQQLTATVRAQSRLQTVDQFKNIIVKSQSDGAVVRIKDVARVEMGSEDY
                     TAIGKLNGHPSAGVAVMLSPGANALNTATLVKDKIAEFQRNMPQGYDIAYPKDSTEFI
                     KISVEDVIQTLFEAIVLVVCVMYLFLQNLRATLIPALAVPVVLLGTFGVLALFGYSIN
                     TLTLFAMVLAIGLLVDDAIVVVENVERIMRDEGLPAREATEKSMGEISGALVAIALVL
                     SAVFLPMAFFGGSTGVIYRQFSITIISAMLLSVVVALTLTPALCGSVLQHVPPHKKGF
                     FGAFNRFYRRTEDKYQRGVIYVLRRAARTMGLYVVLGGGMALMMWKLPGSFLPTEDQG
                     EIMVQYTLPAGATAARTAEVNRQIVDWFLINEKANTDVIFTVDGFSFSGSGQNTGMAF
                     VSLKNWSQRKGAENTAQAIALRATKELGTIRDATVFAMTPPAVDGLGQSNGFTFELLA
                     NGGTDRETLLQMRNQLIEKANQSPELHSVRANDLPQMPQLQVDIDSNKAVSLGLSLND
                     VTDTLSSAWGGTYVNDFIDRGRVKKVYIQGDSEFRSAPSDLGKWFVRGSDNAMTPFSA
                     FATTRWLYGPERLVRYNGSAAYEIQGENATGFSSGDAMTKMEELANSLPAGTTWAWSG
                     LSLQEKLASGQALSLYAVSILVVFLCLAALYESWSVPFSVILVIPLGLLGAALAAWMR
                     DLNNDVYFQVALLTTIGLSSKNAILIVEFAEAAVAEGYSLSRAALRAAQTRLRPIIMT
                     SLAFIAGVMPLAIATGAGANSRIAIGTGIIGGTLTATLLAIFFVPLFFVLVKRLFAGK
                     PRRQE"
     gene            12057..13430
                     /locus_tag="NBI74_001151"
     CDS             12057..13430
                     /locus_tag="NBI74_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HCD1931517.1"
                     /translation="MLRRSLIFLVLLSAGCVSLDPHYSTPESPIPATLPGAQGQGKAI
                     SHDWQQVIHDPRLQQVVTIALNSNRDVQKAIADIDSARALYGQTNASLFPTVNAALSS
                     TRSRSLANGTGTTAEADGTVSSYTLDLFGRNQSLSRAARETWLASEFTAQNTRLTLIA
                     EISTAWLTLAADNSNLALAKETMASAENSLKIIQRQQQVGTAAATDVSEAMSVYQQAR
                     ASVASYQTQVMQDKNALNLLAGTTLAENLLPGTLESLPEQMISLVPAGVSSDVLLRRP
                     DIQEAEHNLKSANADIGAARANFFPTISLTASAGVGSDALSSLFSHGMQIWSFAPSVT
                     LPLFTGGSNLAQLRYAEAQKRGLIATYEKTVQSAFKDVANALARRTTLEEQLDAQRQY
                     VKAEQQTVDVGLRRYQAGVGDYLTVLTAQRSLWSAQQELLALQLTDFTNRITLWQSLG
                     GGMSSLK"
     gene            13439..14602
                     /locus_tag="NBI74_001152"
     CDS             13439..14602
                     /locus_tag="NBI74_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug effflux MFS transporter"
                     /protein_id="HCD1931518.1"
                     /translation="MARVSLSWALILGLLAGIGPMCTDLYLPALPEMSEQLAATTTIT
                     QLTLTASLIGLGVGQLLFGPLSDKIGRKRPLILSLLLFIVSSILCATTNNIYWLVVWR
                     FIQGIAGAGGSVLSRSIARDKYQGVTLTQFFALLMTVNGLAPVLSPVLGGYIVSTFDW
                     RTLFWVMAEISTVLLLGCLLFIHETLPENKRGSSLLLTGRSVVQNRRFMRFCLIQSFM
                     LAGLFAYIGSSSFVLQKEFGFSPMQFSLVFGLNGTGLIIASWIFSRLARRINAMTLLR
                     GGLIAAILCALLTVLCAWIQLPIPALVALFFTIAFCSGIGTVGGAEAMSAVGTQESGT
                     ASALMGMSMFVFGGIAAPLSGIGGETLLKMSLAITVCYTLALLVALTRIDNQK"
     gene            complement(14650..15456)
                     /gene="sapF"
                     /locus_tag="NBI74_001153"
     CDS             complement(14650..15456)
                     /gene="sapF"
                     /locus_tag="NBI74_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapF"
                     /protein_id="HCD1931519.1"
                     /translation="MIETLLEVRNLSKTFRYRTGWFRRQTVEAVKPLSFTLREGQTLA
                     IIGENGSGKSTLAKMLAGMIEPTSGELLIDDHPLHFGDYSFRSQRIRMIFQDPSTSLN
                     PRQRISQILDFPLRLNTDLEPEQRRKQIIETMRMVGLLPDHVSYYPHMLAPGQKQRLG
                     LARALILRPKVIIADEALASLDMSMRSQLINLMLELQEKQGISYIYVTQHIGMMKHIS
                     DQVLVMHQGEVVERGSTADVLASPLHELTKRLIAGHFGEALTADAWRKDR"
     gene            complement(15458..16450)
                     /gene="sapD"
                     /locus_tag="NBI74_001154"
     CDS             complement(15458..16450)
                     /gene="sapD"
                     /locus_tag="NBI74_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapD"
                     /protein_id="HCD1931520.1"
                     /translation="MPLLDIRNLTIEFKTGDEWVKAVDRVSMTLTEGEIRGLVGESGS
                     GKSLIAKAICGVNKDNWRVTADRMRFDDIDLLRLSARERRKLVGHNVSMIFQEPQSCL
                     DPSERVGRQLMQNIPAWTYKGRWWQRFGWRKRRAIELLHRVGIKDHKDAMRSFPYELT
                     EGECQKVMIAIALANQPRLLIADEPTNSMEPTTQAQIFRLLTRLNQNSNTTILLISHD
                     LQMLSQWADKINVLYCGQTVETAPSKELVTMPHHPYTQALIRAIPDFGSAMPHKSRLN
                     TLPGAIPLLEQLPIGCRLGPRCPYAQRECIVTPRLTGAKNHLYACHFPLNMEKE"
     gene            complement(16450..17340)
                     /gene="sapC"
                     /locus_tag="NBI74_001155"
     CDS             complement(16450..17340)
                     /gene="sapC"
                     /locus_tag="NBI74_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease SapC"
                     /protein_id="HCD1931521.1"
                     /translation="MPYDSVYSEKRPPGTLRTAWRKFYSDASAMVGLYGCAGLAVLCI
                     FGGWFAPYGIDQQFLGYQLLPPSWSRYGEVSFFLGTDDLGRDVLSRLLSGAAPTVGGA
                     FVVTLAATICGLVLGTFAGATHGLRSAVLNHILDTLLAIPSLLLAIIVVAFAGPSLSH
                     AMFAVWLALLPRMVRSIYSMVHDELEKEYVIAARLDGASTLNILWFAVMPNITAGLVT
                     EITRALSMAILDIAALGFLDLGAQLPSPEWGAMLGDALELIYVAPWTVMLPGAAIMIS
                     VLLVNLLGDGVRRAIIAGVE"
     gene            complement(17327..18292)
                     /gene="sapB"
                     /locus_tag="NBI74_001156"
     CDS             complement(17327..18292)
                     /gene="sapB"
                     /locus_tag="NBI74_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease SapB"
                     /protein_id="HCD1931522.1"
                     /translation="MIIFTLRRILLLIVTLFLLTFVGFSLSYFTPHAPLQGASLWNAW
                     VFWFNGLIHWDFGVSSINGQPIAEQLKEVFPATMELCILAFGFALIVGIPVGMIAGIT
                     RHKWQDNLINAIALLGFSIPVFWLALLLTLFCSLTLGWLPVSGRFDLLYEVKPITGFA
                     LIDAWLSDSPWRDEMIISAIRHMVLPVITLSVAPTTEVIRLMRISTIEVYDQNYVKAA
                     ATRGLSRFTILRRHVLHNALPPVIPRLGLQFSTMLTLAMITEMVFSWPGLGRWLINAI
                     RQQDYAAISAGVMVCGSLVIIVNVISDILGAMANPLKHKEWYALR"
     gene            complement(18289..19932)
                     /gene="sapA"
                     /locus_tag="NBI74_001157"
     CDS             complement(18289..19932)
                     /gene="sapA"
                     /locus_tag="NBI74_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein SapA"
                     /protein_id="HCD1931523.1"
                     /translation="MRQVLSSLLVIAGLVSGQAIAAPESPPHADIRDSGFVYCVSGQV
                     NTFNPSKASSGLIVDTLAAQFYDRLLDVDPYTYRLMPELAESWEVLDNGATYRFHLRR
                     DVPFQKTDWFTPTRKMNADDVVFTFQRIFDRNNPWHNVNGSNFPYFDSLQFADNVKSV
                     RKLDNHTVEFRLAQPDASFLWHLATHYASVMSAEYAGKLEKEDRQEQLDRQPVGTGPY
                     QLSEYRAGQYIRLQRHDDFWRGKPLMPQVVVDLGSGGTGRLSKLLTGECDVLAWPAAS
                     QLSILRDDPRLRLTLRPGMNVAYLAFNTAKPPLNNPAVRHALALAINNQRLMQSIYYG
                     TAETAASILPRASWAYDNEAKITEYNPAKSREQLKALGLENLTLKLWVPTRSQAWNPS
                     PLKTAELIQADMAQVGVKVVIVPVEGRFQEARLMDMSHDLTLSGWATDSNDPDSFFRP
                     LLSCAAIHSQTNLAHWCDPKFDSVLRKALSSQQLAARIEAYDEAQSILAQELPILPLA
                     SSLRLQAYRYDIKGLVLSPFGNASFAGVYREKQDEVKKP"
     gene            complement(20245..20490)
                     /locus_tag="NBI74_001158"
     CDS             complement(20245..20490)
                     /locus_tag="NBI74_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmjA family protein"
                     /protein_id="HCD1931524.1"
                     /translation="MNHDIPLKYYDIADEYATECAEPVADAERTPLAHYFQLLLTRLM
                     NNEEISEEAQHEMAAEAGINPVRIDEIAEFLNQWGNE"
     gene            complement(20624..20761)
                     /locus_tag="NBI74_001159"
                     /pseudo
     CDS             complement(20624..20761)
                     /locus_tag="NBI74_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415812.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Putrescine importer PuuP"
     gene            complement(20915..21892)
                     /gene="pspF"
                     /locus_tag="NBI74_001160"
     CDS             complement(20915..21892)
                     /gene="pspF"
                     /locus_tag="NBI74_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein operon transcriptional
                     activator"
                     /protein_id="HCD1931525.1"
                     /translation="MAEYKDNLLGEANSFLEVLEQVSHLAPLDKPVLIIGERGTGKEL
                     IASRLHYLSSRWQGPFISLNCAALNENLLDSELFGHEAGAFTGAQKRHPGRFERADGG
                     TLFLDELATAPMMVQEKLLRVIEYGELERVGGSQPLQVNVRLVCATNADLPAMVNEGT
                     FRADLLDRLAFDVVQLPPLRERESDIMLMAEHFAIQMCREIKLPLFPGFTEHARETLL
                     NYRWPGNIRELKNVVERSVYRHGTSDYPLDDIIIDPFKRRPSEEAIAVSENTSLPTLP
                     LDLREFQMQQEKELLQVSLEQGKYNQKRAAELLGLTYHQFRALLKKHQL"
     gene            22059..22727
                     /gene="pspA"
                     /locus_tag="NBI74_001161"
     CDS             22059..22727
                     /gene="pspA"
                     /locus_tag="NBI74_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspA"
                     /protein_id="HCD1931526.1"
                     /translation="MGIFSRFADIVNANINALLEKAEDPQKLVRLMIQEMEDTLVEVR
                     STSARALAEKKQLTRRIEQASAREIEWQEKAELALLKEREDLARAALIEKQKLTDLIK
                     SLEHEVTLVDDTLVRMKKEIGELENKLSETRARQQALMLRHQAANSSRDVRRQLDSGK
                     LDEAMARFESFERRIDQMEAEAESHSFGKQKSLDDQFAELKADDAISEQLAQLKAKMK
                     QDNQ"
     gene            22781..23005
                     /gene="pspB"
                     /locus_tag="NBI74_001162"
     CDS             22781..23005
                     /gene="pspB"
                     /locus_tag="NBI74_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspB"
                     /protein_id="HCD1931527.1"
                     /translation="MSALFLAIPLTIFVLFVLPIWLWLHYSNRSGRSELSQSEQQRLA
                     QLADEAKRMRERIQALESILDAEHPNWRDR"
     gene            23005..23364
                     /gene="pspC"
                     /locus_tag="NBI74_001163"
     CDS             23005..23364
                     /gene="pspC"
                     /locus_tag="NBI74_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspC"
                     /protein_id="HCD1931528.1"
                     /translation="MAGINLNKKLWRIPQQGMVRGVCAGIANYFDVPVKLVRILVVLS
                     IFFGLALFTLVAYIILSFALDPMPDNMAFGEQLPSSSELLDEVDRELAASETRLREME
                     RYVTSDTFTLRSRFRQL"
     gene            23373..23594
                     /gene="pspD"
                     /locus_tag="NBI74_001164"
     CDS             23373..23594
                     /gene="pspD"
                     /locus_tag="NBI74_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspD"
                     /protein_id="HCD1931529.1"
                     /translation="MNTRWQQAGQKVKPGFKLAGKLVLLTALRYGPAGVAGWAIKSVA
                     RRPLKMLLAVALEPLLSRAANKLAQRYKR"
     gene            23669..23983
                     /gene="pspE"
                     /locus_tag="NBI74_001165"
     CDS             23669..23983
                     /gene="pspE"
                     /locus_tag="NBI74_001165"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase PspE"
                     /protein_id="HCD1931530.1"
                     /translation="MFKKGLLALTLVFSLPVFAAEHWIDVRVPEQYQQEHVQGAINIP
                     LKEVKERIATAVPDKNDTVKVYCNAGRQSGQAKEILSEMGYTHVENAGGLKDIAMPKV
                     KG"
     gene            24194..25873
                     /gene="ycjM"
                     /locus_tag="NBI74_001166"
     CDS             24194..25873
                     /gene="ycjM"
                     /locus_tag="NBI74_001166"
                     /EC_number="2.4.1.352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosylglycerate phosphorylase"
                     /protein_id="HCD1931531.1"
                     /translation="MQQKITDYLDEIYGGTFTATHLQKLVTRLESAKRLITQRRKKHW
                     DESDVVLITYADQFHSNDLKPLPTFNQFYPQWLQRIFSHVHLLPFYPWSSDDGFSVID
                     YHQVASEAGEWQDIQQLGECSHLMFDFVCNHMSAKSEWFKNYLQQQPGFEDFFITVDP
                     KTDLSAVTRPRALPLLTPFQMRDHSTRHLWTTFSDDQIDLNYRSPEVLLAMVDVLLCY
                     LEKGAEYVRLDAVGFMWKEPGTNCIHLEKTHLIIKLLRSIIDNVAPGTVIITETNVPH
                     KDNIAYFGEGDDEAHMVYQFSLPPLVLHAVQKQNVEALCAWAQNLTLPSSNTTWFNFL
                     ASHDGIGLNPLRGLLPENEILALVEVLQQEGALVNWKNNPDGTRSPYEMNVTYMDALS
                     RRECSDEERCARFILAHAILLSFPGVPAIYIQSILGSRNDYAGVEKLGYNRAINRKKY
                     SSKEITTELDNKATLRYSVYYKLSHLITLRRSHKEFHPDNNFTIDAINSFVMRIQRSN
                     ADGDCLTGLFNVSENIQHINITDLHGRDLISEVDILGNEITLHPWQVMWIK"
     gene            25887..27179
                     /locus_tag="NBI74_001167"
     CDS             25887..27179
                     /locus_tag="NBI74_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HCD1931532.1"
                     /translation="MIKSKIVLLSALVSCALISGCKEENKNNVSIEFMHSSVEQERQA
                     VISKLIARFEKENPGITVKQVPVEEDAYNTKVITLSRSGSLPEVIETSHDYAKVMDKE
                     QLIDRQAVATVISNVGEGAFYDGVLRIVRTEDGSAWTGVPVSAWIGGIWYRKDVLAKA
                     GLEEPKNWQQLLDVAQKLNDPANKKYGIALPTAESVLTEQSFSQFALSNQANVFNAEG
                     KITLDTPEMMQALTYYRDLAANTMPGSNDIMEVKDAFMNGTAPMAIYSTYILPAVIKE
                     GDPKNVGFVVPTEKNSAVYGMLTSLTITAGQKAEETEAAEKFVTFMEQADNIADWVMM
                     SPGAALPVNKAVVTTATWKDNDVIKALGELPNQLISELPNIQVFGAVGDKNFTRMGDV
                     TGSGVVSSMVHNVTVGNADLPGTLQASQKKLDELIEQH"
     gene            27200..28081
                     /locus_tag="NBI74_001168"
     CDS             27200..28081
                     /locus_tag="NBI74_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HCD1931533.1"
                     /translation="MNRLFSGRSDMPFALLLLAPSLLLLGGLVAWPMVSNIEISFLRL
                     PLNPNIESTFVGVSNYVRILSDPGFWHSLWMTVWYTALVVAGSTVLGLAVAMFFNREF
                     RLRKTARSLVILSYVTPSISLVFAWKYMFNNGYGIVNYLGVDLLHLYEQAPLWFDNPG
                     SSFVLVVLFAIWRYFPYAFISFLAILQTIDKSLYEAAEMDGANAWQRFRIVTLPAIMP
                     VLATVVTLRTIWMFYMFADVYLLTTKVDILGVYLYKTAFAFNDLGKAAAISVVLFIII
                     FAVILLTRKRVNLNGNK"
     gene            28068..28910
                     /locus_tag="NBI74_001169"
     CDS             28068..28910
                     /locus_tag="NBI74_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HCD1931534.1"
                     /translation="MATNKRTLSRIGFYCGLALFLIITLFPFFVMLMTSFKSAKEAIS
                     LHPTLLPQQWTLEHYVDIFNPVIFPFVDYFRNSMVVSVVSSVVAVFLGILGAYALSRL
                     RFKGRMTINASFYTVYMFSGILLVVPLFKIITALGIYDTEMALIITMVTQTLPTAVFM
                     LKSYFDTIPDEIEEAAMMDGLNRLQIIFRITVPLAMSGLISVFVYCFMVAWNDYLFAS
                     IFLSSASNFTLPVGLNALFSTPDYIWGRMMAASLVTALPVVIMYALSERFIKSGLTAG
                     GVKG"
     gene            28941..29993
                     /locus_tag="NBI74_001170"
     CDS             28941..29993
                     /locus_tag="NBI74_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HCD1931535.1"
                     /translation="MKKLVATAPRVAALVEYEDRAILANEVKIRVRFGAPKHGTEVVD
                     FRAASPFIDEDFNGEWQMFSPRPANAPRGIEFGKFQLGNMVVGDIIECGSDVNDYAVG
                     DSVCGYGPLSETVIINAVNNYKLRKMPQGSSWKNAVCYDPAQFAMSGVRDANVRVGDF
                     VVVVGLGAIGQIAIQLAKRAGASVVIGVDPITRRCDIARRHGADFCLNPIGADVGKEI
                     KTLTGKQGADVIIETSGYADALQSALRGLAYGGTISYVAFAKPFAEGFNLGREAHFNN
                     AKIVFSRACSEPNPDYPRWSRKRIEETCWELLMNGYLNCEDLIDPVVTFANSPESYMQ
                     YVDQHPEQSIKMGVTF"
     gene            30012..30800
                     /locus_tag="NBI74_001171"
     CDS             30012..30800
                     /locus_tag="NBI74_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415830.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HCD1931536.1"
                     /translation="MKIGTQNQAFFPENILEKFRYIKEMGFDGFEIDGKLLVNNLEEV
                     KAAIKETGLPVTTACGGYDGWIGDFIEERRLNGLKQIERILEALAEVGGKGIVVPAAW
                     GMFTFRLPPMTSPRSLDGDRKMVSDSLRVLEQAAARTGTVVYLEPLNRYQDHMINTLA
                     DARRYIVENDLKHVQIIGDFYHMNIEEDNLAQALHDNRDLLGHVHIADNHRYQPGSGT
                     LDFHALFEQLRADNYQGYVVYEGRIRAEAPAQAYRDSLAWLRTC"
     gene            30810..31865
                     /locus_tag="NBI74_001172"
     CDS             30810..31865
                     /locus_tag="NBI74_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCD1931537.1"
                     /translation="MKSAMTSSPLRVAIIGAGQVADKVHASYYCTRNDLELVAVCDSR
                     LSQAQALAEKYGNASVWDDPQAMLLAVKPDVVSVCSPNRFHYEHTLMALEAGCHVMCE
                     KPPAMTAEQAREMCDTARKQGKVLAYDFHHRFALDTQQLREQVTNGVLGEIYVTTARA
                     LRRCGVPGWGVFTNKELQGGGPLIDIGIHMLDAAMYVLGFPAVKSVTAHSFQKIGTQK
                     SCGQFGEWDPATYSVEDSLFGTIEFHNGGILWLETSFALNIREQSIMNVSFCGDKAGA
                     TLFPAHIYTDNNGELMTLMQREMADDNRHLRSMEAFINHVQGKPVMIADAEQGYIIQQ
                     LVAALYQSAETGTRVEL"
     gene            31862..34129
                     /gene="ycjT"
                     /locus_tag="NBI74_001173"
     CDS             31862..34129
                     /gene="ycjT"
                     /locus_tag="NBI74_001173"
                     /EC_number="2.4.1.230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kojibiose phosphorylase"
                     /protein_id="HCD1931538.1"
                     /translation="MTRPVTLSEPHFSQHTLNKYASLMAQGNGYLGLRASHEEDYTRQ
                     TRGMYLAGLYHRAGKGEINELVNLPDVVGMEIAINGEIFSLSREAWQRELNFASGELR
                     RNVVWRTSNGTGYTIASRRFVSADQLPLIALEITITPLDADASVLISTGIDATQTNHG
                     RQHIDEIQVRVFGQHLMQGIYTTQDGRSDVAISCCCKVSGDVQQCYTAKERRLLQHTC
                     AQLHAGETLTLQKLVWIDWRDDRQAALDEWGSASLRQLEMCAQQSYGQLLAASTENWR
                     QWWQKRRITVNGGDAHDQQALDYALYHLRIMTPAHDERSSIAAKGLTGEGYKGHVFWD
                     TEVFLLPFHLFSDPTVARSLLRYRWHNLPGAQEKARRNGWQGALFPWESARSGEEETP
                     EFAAINIRTGLRQKVASAQAEHHLVADIAWAVIQYWRTTGDESFIAHEGMALLLETAK
                     FWISRAVRVNDRLEIHDVIGPDEYTEHVNNNAFTSYMAYYNVQQALNIARQFGCSDDA
                     FIHRAEMYLKELRLPEIQPDGVLPQDDSFMAKPLINLAKYKVAAGKQTILLDYSRAEV
                     NEMQILKQADVVMLNYMLPEQFSAASCLANLQFYEPRTIHDSSLSKAIHGIVAARCGL
                     LTQSYQFWREGTEIDLGADPPSCDDGIHAAATGAIWLGAIQGFAGVSVRDGELHLNPA
                     LPEQWQQLSFPLFWQGCELQVTLDAQRIAIRTSAPVSLRLNGQLIYVAEESVFCLGDF
                     ILPFNGTATTHQEGE"
     gene            34126..34785
                     /gene="pgmB"
                     /locus_tag="NBI74_001174"
     CDS             34126..34785
                     /gene="pgmB"
                     /locus_tag="NBI74_001174"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HCD1931539.1"
                     /translation="MKLQGVIFDLDGVITDTAHLHFQAWQQIAAEIGISIDAQFNEFL
                     KGISRDESLWRILQHGGKEGDFNPQERAQLAYRKNLLYVHSLHELTVNAVLPGIHNLL
                     VDLRAQQIPVGLASVSLNAPTILAALELREFFTFCADASQLKNSKPDPEIFLAACAGL
                     GVPPQACIGIEDAQAGIEAINASGMRSVGIGAGLTGAQLLLPSTDSLTWPRLSAFWQN
                     V"
     gene            34799..35881
                     /locus_tag="NBI74_001175"
     CDS             34799..35881
                     /locus_tag="NBI74_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000057971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCD1931540.1"
                     /translation="MAQLSLQHIQKIYDNQVHVVKDFSLEIADKEFIVFVGPSGCGKS
                     TTLRMIAGLEEISGGDLLIDGKRMNDVPAKARNIAMVFQNYALYPHMTVYDNMAFGLK
                     MQKIAKEVIDERVNWAAQILGLREYLKRKPGALSGGQRQRVALGRAIVREAGVFLMDE
                     PLSNLDAKLRVQMRAEISKLHQKLNTTMIYVTHDQTEAMTMATRIVIMKDGIVQQVGA
                     PKTVYNQPANMFVAGFIGSPAMNFIRGTIDGDKFVTETLKLTIPEEKLAVLKTQESLH
                     KPIVMGIRPEDIHPDAQEENNISAKISVAELTGAEFMLYTTVGGHELVVRAGALNDYH
                     AGENITIHFDMTKCHFFDAETEIAIC"
     gene            35926..36831
                     /gene="ompG"
                     /locus_tag="NBI74_001176"
     CDS             35926..36831
                     /gene="ompG"
                     /locus_tag="NBI74_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monomeric porin OmpG"
                     /protein_id="HCD1931541.1"
                     /translation="MKKLLPCTALVMCAGMVCAQAEEKNDWHFNIGAMYEIENVEGYG
                     EDMDGLAEPSVYFNAANGPWRISLAYYQEGPVDYSAGKRGTWFDRPELEVHYQFLESD
                     DFSFGLTGGFRNYGYHYVDEPGKDTANMQRWKIAPDWDVKLTDDLRFNGWLSMYKFAN
                     DLNTTGYADTRVETETGLQYTFNETVALRVNYYLERGFNMDDSRNNGEFSTQEIRAYL
                     PLTLGNHSVTPYTRIGLDRWSNWDWQDDIEREGHDFNRVGLFYGYDFQNGLSVSLEYA
                     FEWQDHDEGDSDKFHYAGVGVNYSF"
     gene            complement(36882..37880)
                     /locus_tag="NBI74_001177"
     CDS             complement(36882..37880)
                     /locus_tag="NBI74_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCD1931542.1"
                     /translation="MSPTIYDIARVAGVSKSTVSRVLNKQTNISPEAREKVLRAIEEL
                     QYQPNKLARALTSSGFDAIMVISTRSTKTTAGNPFFSEVLHAITAKAEEEGFDVILQT
                     SHNPAEDLQKCESKIKQKMIKGIIMLSSPADESFFAQLDKYEIPVVVIGKVEGQYSHV
                     YSVDTDNYGDSIALTNALIESGHKNIACLHAPLDVQVSVDRVNGYKQSLATHNIAVRD
                     EWIVDGGYTHETALKAARELLSQSPLPEAVFATDSLKLMSIYRAAAEKNIAIPQQLAV
                     VGYSNETLSFILTPAPGGIDVPTQELGQQSCELLFQLIAGKPSPQNITVATHMSLK"
     gene            38036..39433
                     /locus_tag="NBI74_001178"
     CDS             38036..39433
                     /locus_tag="NBI74_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcjX family protein"
                     /protein_id="HCD1931543.1"
                     /translation="MKRLKNELNALVNRGVDRHLRLAVTGLSRSGKTAFITAMVNQLL
                     NIHAGARLPLLSAVREERLLGVKRIPQRDFGIPRFTYDEGLAQLYGDPPAWPTPTRGV
                     SEIRLALRFKSNDSLLRHFKDTSTLYLEIVDYPGEWLLDLPMLAQDYLSWSRQMTGLL
                     NGQRGEWSAKWRMMCEGLDPLAPADENRLADIAAAWTEYLHHCKQQGLHFIQPGRFVL
                     PGDMAGAPALQFFPWPDVDAWGESKLAQADKHTNAGMLRERFNYYCEKVVKGFYKNHF
                     LRFDRQIVLVDCLQPLNSGPQAFNDMRLALTQLMQSFHYGQRTLFRRLFSPVIDKLLF
                     AATKADHVTIDQHANMVSLLQQLIQDAWQNAAFEGISMDCLGLASVQATTSGMIDVNG
                     EKIPALRGNRLSDGAPLTVYPGEVPARLPGQAFWDKQGFQFEAFRPQVMDVDKPLPHI
                     RLDAALEFLIGDKLR"
     gene            39430..40491
                     /locus_tag="NBI74_001179"
     CDS             39430..40491
                     /locus_tag="NBI74_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01620 family protein"
                     /protein_id="HCD1931544.1"
                     /translation="MTEPLKPRIDFDGPLEVDQNPKFRAQQTFDENQAQNFAPATLDE
                     AQEEEGQVEAVMDAALRPKRSLWRKMVMGGLALFGASVVGQGVQWTMNAWQTQDWVAL
                     GGCAAGALIIGAGVGSVVTEWRRLWRLRQRAHERDEARDLLHSHGTGKGRAFCEKLAQ
                     QAGIDQSHPALQRWYASIHETQNDREVVSLYAHLVQPVLDAQARREISRSAAESTLMI
                     AVSPLALVDMAFIAWRNLRLINRIATLYGIELGYYSRLRLFKLVLLNIAFAGASELVR
                     EVGMDWMSQDLAARLSTRAAQGIGAGLLTARLGIKAMELCRPLPWIDDDKPRLGDFRR
                     QLIGQVKETLQKGKTPSEK"
     gene            40639..42180
                     /gene="tyrR"
                     /locus_tag="NBI74_001180"
     CDS             40639..42180
                     /gene="tyrR"
                     /locus_tag="NBI74_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TyrR"
                     /protein_id="HCD1931545.1"
                     /translation="MRLEVFCEDRLGLTRELLDLLVLRGIDLRGIEIDPIGRIYLNFA
                     ELEFESFSSLMAEIRRIAGVTDVRTVPWMPSEREHLALSALLEALPEPVLSVDMKSKV
                     DMANPASCQLFGQKLDRLRNHTAAQLINGFNFLRWLESEPQDSHNEHVVINGQNFLME
                     ITPVYLQDENDQHVLTGAVVMLRSTIRMGRQLQNVAAQDVSAFSQIVAVSPKMKHVVE
                     QAQKLAMLSAPLLITGDTGTGKDLFAYACHQASPRASKPYLALNCASIPEDAVESELF
                     GHAPEGKKGFFEQANGGSVLLDEIGEMSPRMQAKLLRFLNDGTFRRVGEDHEVHVDVR
                     VICATQKNLVELVQKGVFREDLYYRLNVLTLNLPPLRDCPQDIMPLTELFVARFADEQ
                     GVPRPKLAADLNTVLTRYAWPGNVRQLKNAIYRALTQLDGYELRPQDILLPDYDAATV
                     AVGEDAMEGSLDEITSRFERSVLTQLYRNYPSTRKLAKRLGVSHTAIANKLREYGLSQ
                     KKNEE"
     gene            complement(42224..42730)
                     /gene="tpx"
                     /locus_tag="NBI74_001181"
     CDS             complement(42224..42730)
                     /gene="tpx"
                     /locus_tag="NBI74_001181"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HCD1931546.1"
                     /translation="MSQTVHFQGNPVTVANSIPQAGSKAQTFTLVAKDLSDVTLGQFA
                     GKRKVLNIFPSIDTGVCAASVRKFNQLATEIDNTVVLCISADLPFAQSRFCGAEGLNN
                     VITLSTFRNAEFLQAYGVAIADGPLKGLAARAVVVIDENDNVIFSQLVDEITTEPDYE
                     AALAVLKA"
     gene            42849..43814
                     /gene="ycjG"
                     /locus_tag="NBI74_001182"
     CDS             42849..43814
                     /gene="ycjG"
                     /locus_tag="NBI74_001182"
                     /EC_number="5.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-Ala-D/L-Glu epimerase"
                     /protein_id="HCD1931547.1"
                     /translation="MRTVKVFEEAWPLHTPFVIARGSRSEARVVVVELEEEGIKGTGE
                     CTPYPRYGESDASVMAQIMSVVPQLEKGLTREELQKILPAGAARNALDCALWDLAARK
                     QQQSLADLIGITLPETVTTAQTIVIGTPDQMANSASTLWQAGAKLLKVKLDNHLISER
                     MVAIRTAVPDATLIVDANESWRAEGLAARCQLLADLGVAMLEQPLPAQDDAALENFIH
                     PLPICADESCHTRSNLKALKGRYEMVNIKLDKTGGLTEALALATEARAQGFRLMLGCM
                     LCTSRAISAALPLVPQVSFADLDGPTWLAVDVEPALQFTTGELHL"
     gene            complement(43789..44517)
                     /gene="mpaA"
                     /locus_tag="NBI74_001183"
     CDS             complement(43789..44517)
                     /gene="mpaA"
                     /locus_tag="NBI74_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415842.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide amidase MpaA"
                     /protein_id="HCD1931548.1"
                     /translation="MTVTRPRAERGAFPPGTEHYGRSLLGAPLIWFPAPAASHESGLI
                     LAGTHGDENSSIVTLSCALRTLTPSLRRHHVVLCVNPDGCQLGLRANANGVDLNRNFP
                     AANWKEGETVYRWNSAAEERDVVLLTGDKPGSEPETQALCQLIHRIQPAWVVSFHDPL
                     ACIEDPRHSELGEWLAQAFELPLVTSVGYETPGSFGSWCADLNLHCITAEFPPISSDE
                     ASEKYLFAMANLLRWHPKDAIRPS"
     gene            complement(44808..45446)
                     /locus_tag="NBI74_001184"
     CDS             complement(44808..45446)
                     /locus_tag="NBI74_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCD1931549.1"
                     /translation="MKNVLILGAGGQIARHVINQLADKQTIKQTLFARQPAKIHKPYP
                     TNSKIIMGDVLNHAALKQAMQGQDVVYANLTGEDLDIQANSVIAAMKACDVKRLIFVL
                     SLGIYDEVPGKFGEWNNAVIGEPLKPFRRAADAIEASGLEYTILRPAWLTDEDIIDYE
                     LTSRNEPFKGTIVSRKSVAALITDIIDKPEKHIGENIGINQPGTDGDKPFFM"
     gene            complement(45517..46437)
                     /locus_tag="NBI74_001185"
     CDS             complement(45517..46437)
                     /locus_tag="NBI74_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCD1931550.1"
                     /translation="MMNNKVSFTNSNNPTISLSAVIYFPPKFDETRQYPAIVVSHPGG
                     GVKEQTAGTYAEKLAEKGFVTIAYDASYQGESGGEPRQLENPYIRTEDVSAVIDYLTT
                     LSYVDNTRIGAMGICAGAGYTANAAIQDRRIKAIGTVSAVNIGSMFRNGWENNVKSID
                     ALPYVDAGSNARTSDISSGEYATMPLAPMKESDAPNEELRQAWEYYHTPRAQYPTAPG
                     YATLRSLNQIITYDAYHMAEVYLTQPIQIVAGSLAGSKWMSDDLYDRASSQDKRYHIV
                     EGANHMDLYDGKVYVAEVISVLAPFFEETL"
     gene            46575..47474
                     /gene="pgrR"
                     /locus_tag="NBI74_001186"
     CDS             46575..47474
                     /gene="pgrR"
                     /locus_tag="NBI74_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator PgrR"
                     /protein_id="HCD1931551.1"
                     /translation="MKREEIADLMAFVVVAEERSFTRAAARLSMAQSALSQIVRRIEE
                     RLGLRLLTRTTRSVVPTEAGEHLLSVLGPMLHDIDSALTSLSDLQNRPSGTIRITTVE
                     HAAKTILLPAMRTFLKSHPEIDIQLTIDYGLTDVVSERFDAGVRLGGEMDKDMIAIRI
                     GPDIPMAIVGSPDYFSRRSAPTSVSQLIDHQAINLYLPTSGTANRWRLIRGGREVRVR
                     MEGQLLLNTIDLIIDAAIDGHGLAYLPYDQVERAIKEKKLIRVLDKFTPDLPGYHLYY
                     PHRRHAGSAFSLFIDRLKYKGAV"
BASE COUNT        11733 a        11558 c        12647 g        11724 t
ORIGIN      
        1 tcaaatagga aaacctgaag gcacgattgt taaatctccc acggtgcctt cgggatgtct
       61 ggcgtagatt cagcaacctg gcgcaagagt tctgctttaa gtatctcgag attatggatc
      121 gcagagtggt aatcgcctgc cacaaggata tctaacacaa tatcaatacg ttcggcaacg
      181 cgctgggcat caaattcaga catcaaggac atccttatag caaaaggggg aaaatgaata
      241 atgcaaaaat tgccgctaaa agagaagggt ttaacagcaa cggctaatta tcatccggga
      301 atacgatata taatgacgga atatagcgct aagtatatat attcatctac ttatgcgcgc
      361 ttcagatagc gtttatacca gcgttcgaag gcgacggcgg gcatcggttt ggcaaacaaa
      421 aatccttgtc gctcattgat cccgttcttg gttaaaaaag catcttcctt tgcactttct
      481 acaccttcgg cgatcacctg aagattcaat gcctgggcca cagcgacgat cgcccggacc
      541 agtgattgcg agacaggctg tttgtgaata tcccgaacaa aaacctggtc aagtttgatg
      601 gcatcgatcg gaaagcgcgc cagttgcgaa agtgaagagt agccggtacc aaaatcgtcc
      661 agatgaactt gcgcacctaa tcggctaaat tgttgaataa cagacagtgc cagttcatca
      721 ttctcaatca ggcagctctc cgtcagttca acatctatag ggcagtattc aaaattgagt
      781 tcctggagaa cttgtttcag ggcggtgaaa atggtttgat cggcgagctg acgtgcagaa
      841 atatttaccg ccactcgcag gttaatgcct ttatcccgcc acttcgctac ctggcgtacg
      901 acatcgagaa tcacccaacg gcctaaaggc acaattagcc ctgattcttc ggcgtaggaa
      961 atgaagtcca acggtggaat caacccacgt tcaggtgact gccaacgtac tagtgcttcc
     1021 agactgcgca cttcgccacg ccaggtgatt ttcggttgat agtgaataac caactgatcg
     1081 ttttccagtg ctttacgcaa gttggtatcc agccagagat attcaaatac ccgctgattc
     1141 atttctgggg taaaaacgca aaattgtcct cgtccgcctt ccttcgctgt gtacattgct
     1201 gtgtcggcgt gacgaataat agccgcgctg tccgaaccat gttcgggaga gagtgaaata
     1261 cctactgaac agctggtata aacttcaatt aaaccaatgc ggaaggggag ccgtaagcgg
     1321 gtcaaaattc gtgatgccat tgcttccagc gcgctttgtg aggtattgga tgccagtacc
     1381 agaaactcat cgccacctgg ccgcgccaat acctggtcat gttcgagaca gcttaaaata
     1441 gccaatgaca cgtcgcgtaa taactggtca ccaaacaaat gcccataggc gtcgttgacc
     1501 tttttaaaat tatccaaatc aagatacaca accccaactt tattgttatc tgcctgatta
     1561 atagcgtgat cgattaactc ctgcattgcg ttacgattcg gcagtccggt gatactgtcg
     1621 gtatttgcca gaatacgcag tcgctcctga gcgcggcgct cttcggtaat gtcggtgccg
     1681 gaacagatta aaaaaatctc gtttttgcca ctgccgctgt ggacaaattt attgcgaaac
     1741 agaaacagcc gctggccttt acgtgttggt atccacagtt cgacttcata tgcattgccg
     1801 cttcgaaaaa atacacggtt attgcgcctg gatgccgcag cttcacgacg actcataaac
     1861 agtttaaaca cgctttgccc aatgacgtcg tgttctttca agcctgtgta atcttcacat
     1921 aaccgattaa agcgctggat attcccccgg ctgtcgagaa tcactattgc ggaatgtgct
     1981 tcagaaacta cctgctcagc aaaggaaagc ccatgtgata aatcacgagc aaccgccggt
     2041 gtgtcatgcc atgcagacgc gttgcctccc cattctcgtt tattgatttt tcgtcctacc
     2101 agatgcacag aaagatcgct ttcatcgacg gtcagactca tcatcaggct ggaggtgatg
     2161 accgtcattt ccctgatgcg agcagcctgt tcggcagata gttgcaaaat acgatcgggt
     2221 tctgtgattt cggtggtaga aaagcgcaaa acatcgctgc tttccgtcaa ccgccagtat
     2281 ggattgtgcg atccgagaaa gttgtacaac gttgtggact ccctaacggt tttcatgatg
     2341 catatcctcc ggttaacagc ggagtccgca gttaaataaa aggaacaaaa tgacttacat
     2401 gaaattaacg gcggctaaac acattgtttt tttatatatt tatttgtaat ccagttttgg
     2461 aaaaacgcca gttttcaaac gaaagtcagt taaaaatctg ccgggatatg acgaaggttg
     2521 agcggggaaa taaacggccc atccatgagg aatgggccgt aaaaggagat tacgcgactg
     2581 ggcgcgcaat aatgctgcgg gtttccatgc ggacttcggc aatggtgacg tcaataacgt
     2641 cagtcacttt gtaagccgtt tcacctttaa tttgtacagt gccgttttcc tggctgcaaa
     2701 ccagttcatc gcgcacagcg tgtaagaaag gtgctggaat aaaggcgata gcgccgttat
     2761 caaccaaacg aacacgcatg ccgccacggc tgatatcgac aatttccgcc gcgaaacggg
     2821 tgtcggtccc ggctttgtct ttcaggaagc gtgcgtataa ccagtcgcca acatcacgtt
     2881 ctgccatccg gttgagacgg cgacgctcgg ccatttggac agtgatttca tcctgtggac
     2941 gcgttgcagt ttcgcctttg ataaccgctt tcagcagacg gtggttgatc atgtcgccat
     3001 atttacggat cggcgaagtc caggtggcgt atgcttccag accgaggcca aagtgaggac
     3061 cgggttcagt gctaatttca gcaaatgact ggaagcgacg aatgcggctg tcgaggaaac
     3121 cagttggttg cgcatccagt tcacgacgca gtttgcagaa accgtccagc gtgagcactt
     3181 cttcagcatc gacatgcaga ccgtgcgttt tcagcaacgc tgccagcgcg tcggcattcg
     3241 ccggatcaaa gcccatatgc acgttataga tgccaaaacc gagcttatcg cgcagtacgc
     3301 gggccgcaca aatgttagcg gcaatcatcg cttcttcgac gatacggttg gcaatgcgac
     3361 gaggctcggc aacgatatcc agcacttcac ctttttcacc gagaataaag cggtaatccg
     3421 ggcgatcttt aaacaccagt gcgtggttat gacgccactc gccgcggcgt tggcaaattt
     3481 gtgctagcaa acggacttgt tcggcaattg cttcgctttc aggctgccag tcaccggtat
     3541 tttccagcca gtcagaaacc tggtcataca ccagcttcgc tttggattcg atggtggcgg
     3601 caaagaattc gatattatct tcaatggtgc catcagtgga gagcgtcatg cggcatgcca
     3661 gtaccgggcg gacttcattg gcgcgcagtg agcagagatc gtcagaaagc tcgcgaggca
     3721 gcatagggat gttgaagcca ggcagatagt tggtgaatgc gcgaattttc gcggctttgt
     3781 ccagcttgct gccttcagca atccacgcgg ttggatcggc aatcgccaca atcagctgaa
     3841 gtttgccatc cggcaacgcc ttagcgaaaa gggcgtcatc catatcttct gtgctggcac
     3901 tgtcgatggt gacaaaatcc agcgcagtca gatcttcacg aaccagacct tcatcgagca
     3961 tttcggtagc aacgccgtct ggtgcttctt tttccagatt atgacgcgca agggtaaccc
     4021 accacggtac aaagtgatcg tcaccaaaag tgatgtattg tgtcagttca gcatagaaag
     4081 aacgatcgcc tttcagcgga tgacggcgca tttcggcaac cgcccagtca ccttctttaa
     4141 actcgtggtt caggccacgg gctgcgcggc aaggaatggc gtcttttaag agtggatgat
     4201 caggaacgat ggccagacgg tcatttttgc cctgaacttt acccacgaaa cgagtcagga
     4261 acggttcaac cagttcttct ggctctgcgg attcacgttc tttttcactg tggatcaccg
     4321 cgataattcg gtcgccatgc atgacttttt tcatctgcgg cggcggaatg aaataacttt
     4381 tttgcgcgtc gacttccagg aagccaaagc ctttttctgt ggcttttacc accccttcag
     4441 cgcgtggcgt ctgggaatgc agttgctgtt taagctgcgc tagcagcggg ttgtcctgaa
     4501 acataattgt ctattttggt ggccattaga gcggctgaca gttttacgcg aatctgtctg
     4561 acgcggcaag gttaatatgt ctcacccaac gcgattttta agcgatttat ccagccacac
     4621 agcccgctcc ataccagcag attaataatc tgcgttgatg atttttcgtg ttcgagtaat
     4681 gggctgaact gggcggcgct aaagcgatcg ggtgctcggg tcagccattg tatcgcctga
     4741 acggtagcgc gctcaacaga atgacggtct gcccatcggt taatttcatt ttcgcccttg
     4801 cgcagaaggg ggctgatttt tttatattca tttgatgaat tcatacaggc ggcaaaacaa
     4861 gcaggactgc catttatccg cgcgcataac aacgccgcaa gctgattatc cggctgtgta
     4921 ttgacccgtg tgctgtttaa caacttttcg agaagggaaa gtgaaatagg gtggagagag
     4981 tgtgtgttgg ccagttggca aagttctgca gttgaacttt ttgtgtgctc ttcagggagt
     5041 ttttccacct catagctgtt tcgccagcgt attgattcat cagcaaacag tgacgcatcg
     5101 gcatagtttt gtatctccag cccgggtaac cagcgtaccg gatgtccaag ataagcctga
     5161 aatgtcgcaa tggtccgcgc ctgaaagcca ataaaaccaa tgatttggtt aatgattaca
     5221 cagtcccagg cagtaagacc aacgtcattc agatgctggc gcgatcggtt gtcgataata
     5281 gaaggcgagc tagccagttg gcgggcatat tgcgtgattt gtgccaaccg atgattactt
     5341 tccctggagg aatcaggccc ggggagcggc gtaagtcgcg ccgaatagta gttacataac
     5401 cgttggacgc cgcaaacctg agcaaccgtc agggccgtcg acaagcgttc ataagcggtg
     5461 aaggtctgtg aacgagtcac ggtaagcggg tcagggaaca actctactgc aagaacgcgc
     5521 gcagggacca gccatgaaag gaagggtgcc agcgtttctt catcaaggat cacgtcgagc
     5581 agaaagggat cgctgacctt tgcggcttcc gggaccagag gcagaacgtc atactccgca
     5641 gtactggatt gcgtttcatg ataccagtgg cttttgccgg tgatgtggcg ttgttccatg
     5701 ggcgctcctt ggtcgtaaag gaaatcgtta tcctgacgca aggcgggaag gggagaaaga
     5761 cggatcggcg ataacaaata tcagaaaggt ataacagaga taacgggcgg cagaacgccg
     5821 cccaatattt accaacagaa cgattatttc agttcgagtt cgttcattgc agcgatgctg
     5881 aaaccgccgt caacgtggac cacttcaccg gagataccgg cagagagatc ggagcacagg
     5941 aatgccgcgg agttacccac atcttcaata gtaacggtac ggcgaatcgg ggtaacggct
     6001 tcgcaatgag ccagcatttt gcggaagtct ttgataccgg aagccgccag agtacggatc
     6061 ggaccagcag agatggcgtt aacacgcaca ccttccggac ccatcgcgtt cgccatatag
     6121 cgcacgttcg cttccagaga cgcttttgcc agacccataa cgttgtaatt cgggatagcg
     6181 cgctcagcgc caaggtagga aagggtcagc agggcagaac ccggattcag catggagcgg
     6241 caagcttttg ccattgcaac gaagctgtag gagctgatgt cgtgggcaat tttgaagcct
     6301 tcacgagtaa cggcgttaac atagtcacca tccagctgat cgccaggtgc aaaaccaata
     6361 gagtgtacga aaccgtcaaa tttcggccaa actttcccca gttcagcgaa catggtgtcg
     6421 atgctggtat cttcggcaac atcgcactgc agaacgatgt cagaacccaa ttgagcggca
     6481 aattcttcta cgcggccttt cagtttgtcg ttctggtagg tgaatgccag ttcagctcct
     6541 tcgcggtgca tcgcctgagc gataccgtag gcgatggaca gtttgctggc aacaccggtt
     6601 accagaatgc gcttaccgga aagaaaaccc atagctttaa tccttattgt tgatgcttgt
     6661 tgtgcctgaa aatcaggcga ttcgtcgttt tagtaaacag tacgaacaga taaacggtta
     6721 ttataatcaa cctggctgtg ggtggctata gttgcgcttt ctgtagcgga gagaagctgc
     6781 ggtacagagc attttgcgtt atctactcct gacgcctgca agggggaggt tggggaaata
     6841 tcttatagaa tctgacgttg aaactattcg tattagctcc gataagtttg atgatgaaaa
     6901 cagaactggc gacaacaagt cgctgtcttt ctgatgaatt gactccaact taatattgtc
     6961 aactatcttc acagaatctc cacaatctct tcattatcag aatatgcatc atgaataaga
     7021 taaaaatact ttccattact ttggagaaac gtataattag caaaaatgag aatgatcatt
     7081 ctcaccaagt gataggccag gagacagaaa tgaccagcaa gctggagata cgccacaaac
     7141 agcgtcagga tgaaatcatt aacgccgccc gtcggtgttt tcgtcgctgc ggatttcatg
     7201 ccgccagtat gtcgcaaatt gccagcgaag ctcagcttag cgtgggtcag atatatcgtt
     7261 acttcgccaa caaagacgcc attattgaag agatggttcg ccgtatcatc gactttcgta
     7321 ttgcccaaat ggacattgac gcgcgtaccg atcacttccc ggaagtcctt gccttgcgta
     7381 aatccttaaa tgaggacgac gacgcgctga tgctggaagt ggctgcagaa gccacacgca
     7441 acccgcgggt tatggcaatg cttgaagaag ctgacgccag aatgttcgct aacggttgtg
     7501 cacatatgaa aagaatgcat ccccatctta gcgacgagca cattcgttgc tgcgttgaag
     7561 tgtttgctac catgatggaa ggtacggttt atcgtcgttt aacaccgcaa aaaagcgatc
     7621 cacaacatct gcaggaaata tatcaggata ttgttagtat gttgataaat aaataagaac
     7681 agaattaacc aatagcgggg gatattaatc ccccttattt cattgcgaaa ttcatttcgc
     7741 gatgcgggtt cgttataact gaatatccct gacatatcct cattaatttc aatgaggagg
     7801 gtatttctca ccctggagaa aataatgaag tatatagcaa catctgtagt ggcaatgctg
     7861 ctcttatcgg gttgtgataa tacgcaaagt aacaattcat ccccgtcaga aacagaagtc
     7921 ggcgttgtta cgctcaaatc tcaaccggtt tcggtagtca gtgaattaac cggacgcacc
     7981 agtgctgcgc tcagtgctga agtacgtccg caggttgggg gaattatcca gaaacgctta
     8041 tttaaggaag gtgatctggt caaggctgga cagcctctct accagattga tgcggccagt
     8101 tatcaggctg catggaatga agctcgggca gcattacaac aagcacaggc actggtaaaa
     8161 gccgattgcc agaaagcgca gcgttatgcc cgactggtga aagagaacgg tgtttcacaa
     8221 caggatgctg atgatgctca gtctacctgt gcacaagata aagccagtgt agaggcgaaa
     8281 aaagccgcac tggaaactgc gcgcattaat cttgactgga ccacggtaac cgcaccgatt
     8341 tcggggcgta ttggtatttc gtcggtaacc cctggcgcac tggtgaccgc gtcgcaggat
     8401 acagcgttaa cgactattcg tggtctggat acaatgtatg tcgacctcac tcgctccagt
     8461 gtcgatttat tacgtctgcg taaacagtca ctggcgacca acagtgacac tatgagcgtc
     8521 tcacttattc tggaagatgg cacaacctac agcgaaaaag ggcgtctgga actcaccgaa
     8581 gtcgcggtgg atgagtctac cggttcggtg acattacggg caattttccc caatccacaa
     8641 cagcagttat taccgggaat gtttgttcgc gctcgtgtcg atgaaggcgt gatggaaaac
     8701 gctattctcg cgcctcaaca gggcgttacg cgcgatgcta aaggcaatgc aactgcgctg
     8761 gtggtgaata aagacaataa agtagagcag cgaacgctcg aaacgggaga aacgtatggt
     8821 gataaatggc tggtgctgaa cggcctgcac aacggcgacc gactgattgt tgaaggttct
     8881 gccaaagtca cttcagggca gaccgtcaag gctgttgaag ttcaggctaa tggaggcaac
     8941 gcctgatgtt ttcgcgcttt tttgttcgcc gcccggtctt tgcctgggtt atcgccattt
     9001 tgattatgtt ggcggggatt ctggctattc gcacattacc cgtcgcgcaa tatcctgacg
     9061 ttgcaccgcc gaccattaaa atttcagcca cttatactgg tgcttctgcc gaaacgctgg
     9121 agaacagcgt gactcaggtt atcgagcaac aactcactgg gcttgataat ttactctatt
     9181 tcagctcaac cagtagctct gatggttcgg tcagtattaa tgtgaccttt gaacaaggta
     9241 ccgatccaga tactgcacag gttcaggtgc agaataaaat tcagcaggcg gagtcgcgcc
     9301 tacccagcga agtgcagcaa acgggtgtta cggtggagaa atcacaaagc aactttttgc
     9361 tgattgctgc cgtgtatgac accactgaca aagcctccag ttcggatatt gccgactggc
     9421 tggtcagtaa cgttcaggac ccgctggcgc gtgttgaagg tgtggggagt ctgcaagtct
     9481 ttggcgcgga atacgctatg cgcatctggc tcgacccggc caaactggcg tcttactcgc
     9541 tgatgccttc agacgtgcaa agtgctattg aagcgcaaaa cgtgcaggtt actgccggga
     9601 aaataggggc attgccttca ccggatactc agcaactgac cgcaacggta cgtgcgcagt
     9661 ctcgcttgca gacggtggat cagttcaaaa atattatcgt gaaaagccag tcagacggcg
     9721 cagttgttcg tataaaagat gtggctcgcg ttgagatggg cagtgaagat tataccgcta
     9781 tcggcaaact taacggtcac ccgtctgccg gggttgctgt aatgctttcg cccggtgcga
     9841 atgcgctgaa tacggcgacg ctggtcaagg ataagattgc cgaattccag cggaacatgc
     9901 cgcagggata cgacatcgca tacccgaaag acagcactga atttattaaa atctccgtag
     9961 aggacgtaat tcagacgctg tttgaagcca tcgtattggt ggtttgcgtg atgtatttat
    10021 tcctgcaaaa cctgcgtgcc acactgattc cggcgttagc tgtccccgtc gttttgctgg
    10081 gcacattcgg cgttcttgcg ttgtttggct attcgattaa taccctgacg ttgtttgcaa
    10141 tggtgctggc gatcggcttg ctggtggacg atgccattgt ggtggtagag aacgtcgaac
    10201 gcattatgcg tgacgaaggg ttgcccgcgc gtgaagccac ggaaaaatca atgggcgaga
    10261 tttctggcgc actggttgcc attgcgctgg tgttgtcagc cgtattcctg ccgatggcct
    10321 tctttggggg atctacgggc gtaatttatc gtcagttctc catcaccatc atctccgcaa
    10381 tgctgctttc cgtggtggtg gcattgacct tgactcccgc cctgtgcggt tccgtcctcc
    10441 agcatgttcc accacataaa aaaggctttt tcggtgcatt taaccgcttt taccgccgta
    10501 ctgaagataa atatcagcga ggcgtaattt atgtcctgcg ccgtgcagcc cgaacgatgg
    10561 ggctttatgt cgtgctgggt ggtggaatgg ccctgatgat gtggaaactg ccgggcagtt
    10621 tcttacccac tgaagaccag ggcgaaatca tggtgcagta cacgctgccg gccggtgcga
    10681 ccgctgcccg tacagcagaa gtgaatcgcc agattgttga ctggttcctg attaacgaga
    10741 aagcaaatac cgatgtcatc tttaccgttg atggtttcag ttttagcggc agcggacaga
    10801 acaccgggat ggcgtttgtt tcgttgaaaa actggtctca acgtaaaggg gcagaaaaca
    10861 ccgcccaggc tatcgcccta cgggcaacca aagagctggg cacaattcgt gatgccacag
    10921 tatttgcgat gacgccgcca gccgttgatg ggctggggca aagcaatggt tttacgtttg
    10981 aattgttagc taacggtgga accgatcgtg aaacactgct gcaaatgcgt aatcagttga
    11041 tagaaaaagc gaatcaaagt ccggagttac attctgtacg cgccaatgat ttgccacaaa
    11101 tgccgcaatt gcaggtagat attgatagta ataaagcggt gtcattaggg ttgagtttga
    11161 atgatgtcac cgacaccctg tccagtgcgt ggggcggtac ttatgtgaat gactttattg
    11221 accgtggtcg tgtgaaaaaa gtctacattc agggcgacag cgaatttcgt tccgcgccgt
    11281 cagacttagg taaatggttt gtgcgcggca gcgataacgc catgacacca ttctctgctt
    11341 ttgcgaccac ccgctggctg tatggaccgg aaagactggt gcgctacaac ggctcggcag
    11401 cctatgaaat tcagggcgaa aacgcgactg gctttagttc cggcgatgca atgacaaaaa
    11461 tggaggaact ggcaaacagt cttcctgcgg gaacaacctg ggcctggagt ggtttgtcat
    11521 tgcaggagaa actggccagc ggtcaggcat taagtctgta tgcagtttct attctggtgg
    11581 ttttcctctg ccttgcagca ctgtacgaaa gctggtcagt cccgttctcg gtcattctgg
    11641 tgatccctct ggggcttctt ggcgcggcgc tggcggcctg gatgcgtgat ttaaacaacg
    11701 acgtttactt ccaggtagcg ctattaacta ctatcggctt gtcgtcgaaa aacgccattc
    11761 tgattgtgga atttgctgaa gcggcggttg cagagggcta ttccttgagt cgtgcggcat
    11821 tacgcgcggc gcagactcgt ttacgcccaa tcatcatgac ctcgctggcg tttattgcgg
    11881 gggtaatgcc gctggcgata gcaaccggcg caggggcgaa cagccgcatc gccattggta
    11941 cgggcattat tggcggcacg ctgaccgcta cattactggc cattttcttc gttcctctgt
    12001 tttttgtact ggtgaaacgt ttgtttgccg gtaaaccgcg ccgtcaggag taagttatgt
    12061 tgcgtcgttc tcttattttt ctggtgttgt tgagtgcggg atgtgtctcg ctcgatcctc
    12121 actacagtac gcctgaatcc ccaatcccgg cgaccttgcc gggagcacag ggccagggga
    12181 aggcaatcag ccatgactgg cagcaggtga tccacgatcc ccgattgcag caggtagtaa
    12241 ccattgcact gaacagtaac cgcgatgtgc aaaaagcgat tgctgatatt gactcagcac
    12301 gggcgctcta tgggcaaact aatgcgtcgt tatttccgac ggtgaatgcg gccttaagtt
    12361 cgacccgcag ccgttcgctg gcgaatggta cgggaacaac tgccgaggct gacggcacgg
    12421 tgtccagtta tacgttagat ctgtttggtc gtaatcagag tttatctcgg gcggcgcggg
    12481 aaacctggct tgccagtgaa tttacagccc agaacactcg tttaacgctg attgctgaaa
    12541 tcagcaccgc ctggctgaca ctggcggctg ataacagcaa cctggccctg gcaaaagaaa
    12601 cgatggccag tgcagaaaac tcattgaaaa ttatccagcg ccagcaacag gttggcacgg
    12661 cggcagcgac agatgtcagt gaggcgatga gtgtttatca gcaagcgcgc gccagcgttg
    12721 ccagctatca gacacaagtg atgcaggata aaaatgcgct gaacttactg gcaggtacaa
    12781 cgcttgcgga aaatctgcta ccgggaacac tggaaagcct gccggaacaa atgataagtc
    12841 tggttcctgc gggagtatca tctgatgttc tgctgcgtcg cccggatatc caggaagcgg
    12901 aacataattt gaagagcgcc aatgcagata ttggcgcggc gcgggctaac tttttcccga
    12961 cgatttcgct gactgccagt gccggtgttg gcagtgacgc gttgtcgtcg ttgttcagcc
    13021 acggaatgca gatctggtca tttgcgccat cggtcacatt accgctgttt actggcggaa
    13081 gtaatctggc gcagcttcgt tacgcagaag cacaaaagcg tggactcatt gccacctatg
    13141 aaaaaaccgt tcagagcgca tttaaagatg tcgctaacgc actggcgcgg cggaccacgc
    13201 ttgaagagca actggatgca cagcgccagt atgttaaagc tgaacaacaa acggtcgatg
    13261 ttggcttgcg tcgctatcag gcgggtgttg gtgattacct gaccgtgttg acggcgcagc
    13321 gcagcttgtg gagcgcacag caggagctgc tggcactgca actgactgat tttacaaacc
    13381 gaatcacgct ctggcagtcg ctgggcggcg gtatgtcatc actcaagtaa ggaataatat
    13441 ggctagagtc tctctttcat gggcattgat tcttggtctt ttagccggta tcggcccgat
    13501 gtgtaccgat ctttatttgc cggctttgcc ggagatgtct gagcaactgg cggcaaccac
    13561 gaccataacg caattaaccc tgaccgcatc actgattggt cttggcgtcg gacaactgtt
    13621 atttggccct ctgagcgaca aaatagggcg taaacgtccg ttaatcttgt cgttgctatt
    13681 gtttattgtt tcttccattt tatgcgcgac aacgaacaat atttactggc tggtggtctg
    13741 gcgttttatt caagggatcg cgggggcggg tggttcggtg ctctctcgtt ctattgctcg
    13801 tgacaaatat cagggagtaa cgttgaccca gttttttgcg ctgttaatga cggtgaatgg
    13861 cctggcaccg gtgttgtcgc cagtgctggg cgggtacatt gttagcactt ttgactggcg
    13921 cactttattc tgggtaatgg ctgaaattag caccgtactg ttgctgggct gtctgttatt
    13981 tattcatgag accttgccag aaaataaaag gggctcatca ttgctattaa ccggacgaag
    14041 cgtggtgcag aaccgccgtt ttatgcgctt ttgcctgatt caaagtttta tgctggccgg
    14101 tttgtttgca tatatcggct cttcgtcgtt cgtgttgcag aaggaatttg gctttagtcc
    14161 aatgcaattt agcctggtgt ttggccttaa cggcaccgga cttatcattg cttcatggat
    14221 cttctcgcgc ctggcgcgac ggattaacgc gatgacattg ttgcgaggtg gcctgatagc
    14281 ggcaattttg tgtgcattgc tcacggtctt atgcgcatgg atacaattgc ccattccggc
    14341 actggtggca ttatttttca ccatcgcatt ttgtagcggc atcggcactg ttggcggggc
    14401 agaggctatg agtgcagtag ggacgcagga atctggaacg gcgtctgcgt tgatggggat
    14461 gagcatgttt gtcttcggcg gtatagccgc gccattgtcg ggaattggcg gagaaacact
    14521 gttaaaaatg agtctggcaa ttacggtgtg ttatacgctg gcattgctgg ttgctctcac
    14581 cagaatcgac aaccaaaagt aatgaacacc tcatcctgag gggggcctgt caggatgagg
    14641 tgcaaaacac taacggtcct tacgccacgc atccgccgtc aatgcctcgc caaaatgacc
    14701 ggcgatcagc cgtttggtga gttcatgcag cggcgatgcc agcacatctg cggtgctgcc
    14761 tcgctcgaca acctcgccct gatgcatcac cagcacctgg tcgctaatgt gcttcatcat
    14821 tccgatatgc tgggtaacat aaatatacga aatgccctgt ttttcctgta actccagcat
    14881 cagattaatc aactgcgaac gcatcgacat atccagtgag gcgagggctt catcggcaat
    14941 aatgactttt gggcgcaata tcagcgcgcg cgccagaccc agacgctgtt tttgcccggg
    15001 tgccaacata tgcggatagt aactgacgtg atccggtagc agcccaacca tacgcatcgt
    15061 ttcaataatc tgtttgcgac gctgttccgg ttccaggtcg gtgttcaggc gcagtggaaa
    15121 atccagaatt tgcgagatac gttgacgggg attcaacgag gtggaaggat cctgaaaaat
    15181 catgcgaata cgctgactgc ggaaggaata atcgccaaaa tgcagcggat gatcgtcaat
    15241 caataactcg ccgctggtag gttctatcat tcccgccagc atttttgcca gcgtggattt
    15301 acccgaacca ttctcgccga taatcgccag tgtctggcct tcacgtagcg taaagctcaa
    15361 gggttttacc gcttctacgg tctgacgacg aaaccagccg gtccggtagc ggaacgtttt
    15421 acttaaatta cgcacttcaa gtagcgtttc gatcatctca ctctttctcc atgttcagtg
    15481 ggaaatgaca ggcatagaga tgatttttcg cccccgtcaa acgtggcgtc acaatgcatt
    15541 ctcgttgtgc atacgggcaa cgtggcccca gacgacaacc aatcggtaac tgttccagta
    15601 gcgggatagc gccgggcagc gtattgaggc gacttttatg cggcatcgcg ctgccgaagt
    15661 ctggtatcgc gcggatcagc gcctgggtat aaggatggtg tggcatcgtc accaactcct
    15721 tactcggcgc ggtttccacc gtttgaccgc agtaaagcac gttaatttta tccgcccatt
    15781 ggctaagcat ttgtaagtca tggctgataa gcaaaatagt ggtattgctg ttttggttga
    15841 gacgcgtcag gaggcgaaag atttgcgcct gggttgttgg ctccattgag tttgtcggtt
    15901 cgtcagcaat cagcagacgc ggttgattcg ccagtgcaat ggctatcatc actttctgac
    15961 attcgccttc ggtcaactca tagggaaaac tgcgcatcgc atctttgtga tctttaatcc
    16021 ccacgcggtg cagcagttca atggcacggc gtttgcgcca gccaaaacgc tgccaccaac
    16081 ggcctttata ggtccaggct gggatgtttt gcatcaactg gcgacccaca cgttctgaag
    16141 ggtcaagaca cgactgcggt tcctggaaaa tcatcgacac gttgtgacca accagcttgc
    16201 gccgttcgcg tgcggagaga cgcagcaaat cgatatcatc aaaacgcata cggtcagcag
    16261 taacacgcca gttatcttta ttcaccccac aaatcgcttt cgcaatcaaa cttttaccgg
    16321 aaccggattc gccaacaaga ccgcggattt caccttcggt taaggtcata cttacgcggt
    16381 cgacggcttt aacccactca tcaccggttt taaattcaat ggtcaggtta cgaatatcga
    16441 gtaatggcat tattccaccc ccgcaataat tgcacgacgg acaccgtcgc caaggaggtt
    16501 aaccaacagc acgctaatca taatcgccgc accaggcagc atgacagtcc acggggcgac
    16561 ataaatcagt tccagcgcat caccgagcat cgctccccat tcaggcgagg gcagttgtgc
    16621 gccgagatcg agaaagccca gcgcggcgat atcgagaatt gccatcgaca gtgcgcgggt
    16681 gatctcggtg accagcccgg cggtgatgtt gggcatcaca gcaaaccaga gaatattcag
    16741 cgttgatgcg ccatccagac gggcggcgat aacgtactct ttttccagtt cgtcatgcac
    16801 catgctgtaa atcgaacgta ccatacgtgg cagcagcgcc agccagacag caaacatggc
    16861 gtgagacaaa ctcggtccgg caaacgctac aacgataatt gccagcagca gcgaagggat
    16921 cgccagtaag gtatccagaa tatggttaag caccgctgag cgtaggccgt gggtcgcccc
    16981 ggcgaaggtg cccagtacca ggccacagat cgtcgcggca agcgtcacca caaatgcgcc
    17041 acctacggtt ggcgcagctc cgctcagtaa acggcttaaa acatcgcgtc cgaggtcgtc
    17101 agtccccagg aagaaagaaa cttcgccata gcgcgaccat gacggcggca ataattgata
    17161 accgagaaat tgctggtcga tgccgtaagg cgcaaaccag ccgccaaaaa tacacagtac
    17221 agccagcccc gcgcagccgt acaggccgac catcgcagag gcgtcactat aaaatttgcg
    17281 ccaggcggta cgcagcgtgc ccggcgggcg cttttcgctg tatacgctat cgtaaggcat
    17341 accattcctt atgtttcaga gggttagcca tggcacccaa aatatcagaa atcacgttaa
    17401 caataatgac cagtgagcca cacaccatca ctccggcgga aatggctgcg taatcctgct
    17461 ggcggatggc gttaattaac cagcgcccta aacccggcca gctaaaaacc atttcggtga
    17521 tcatcgccag cgtcagcatg gtggaaaact gtaagcccag gcgaggaata accggaggta
    17581 acgcgttatg cagaacgtgg cgacgcaaaa tagtaaagcg cgacaaaccg cgggtcgccg
    17641 ctgctttaac atagttctgg tcatacactt cgatagtgct gatacgcatc agtcggatca
    17701 cttcagttgt tggcgcaacc gacagggtga tcacgggcaa taccatatgg cggattgcgc
    17761 taatgatcat ttcatcccgc cacggtgagt ccgaaagcca ggcatcaatc aacgcaaaac
    17821 cggtaatcgg tttcacttcg tagagcagat cgaaacgccc tgaaacgggc aaccaaccta
    17881 gcgtgagtga acaaaacagc gtcaacagaa gcgccagcca gaacaccggg attgaaaagc
    17941 ccagtaaggc gatggcgttg atcaagttat cctgccactt atggcgcgta atcccggcaa
    18001 tcatccccac ggggatgcca acaatcagcg caaaaccgaa ggcgaggatg cacagttcca
    18061 tcgttgccgg gaagacctct tttaactgtt cggcaatcgg ttgaccgtta atgctggaaa
    18121 caccaaaatc ccagtggatc aggccattaa accagaacac ccaggcattc cacagtgacg
    18181 cgccttgtaa cggcgcatgt ggggtgaaat agctcaagct gaagccaacg aaggtcagca
    18241 ggaacaaggt cacaatcaat aacaaaatac ggcgtaaggt gaagataatc atggcttttt
    18301 cacctcatcc tgtttctcgc gatacacccc agcaaaggag gcgttaccaa acgggctaag
    18361 taccagacct ttgatatcgt accgataggc ctgcaaacgc aatgacgacg ccagcggcag
    18421 aataggtaat tcctgcgcca gaatactttg cgcttcgtca taggcttcaa tacgcgccgc
    18481 cagctgctgc gaggagagcg ccttacgcaa cacgctgtcg aatttcggat cgcaccagtg
    18541 ggcgaggttg gtctgcgaat gaattgccgc gcagctcagt aacggacgga agaaactgtc
    18601 cgggtcgtta ctgtccgtcg cccaaccgga taacgtcaga tcatggctca tatccatcaa
    18661 ccgcgcctcc tgaaaacgac cttctaccgg cacaatcacc acttttacgc caacctgtgc
    18721 catatccgcc tgaatcagtt cggcggtttt cagtggactg gggttccacg cctgcgaacg
    18781 tgtgggcacc cacagtttca gcgttaagtt ttccagcccc aacgccttca actgttcgcg
    18841 cgattttgcc ggattgtatt cagtaatttt agcctcgttg tcataggccc acgaggcgcg
    18901 cggtaaaata gaggccgctg tttcagccgt accataatag atggattgca tcaggcgctg
    18961 gttattaatt gccagtgcca gcgcatggcg gacagcggga ttatttagcg gcggtttggc
    19021 ggtgttgaat gccagatagg cgacgttcat cccaggacgc agcgttaaac gcaagcgtgg
    19081 gtcgtcacgc aaaatggata gctgactggc agcaggccag gccagaacgt cgcactcccc
    19141 ggtcaggagt ttcgacaatc gtcctgtacc gccagagcct aaatccacca ccacttgtgg
    19201 catcaacggc ttaccacgcc agaaatcatc atgacgttgt aagcgaatat attgcccggc
    19261 gcggtattcc gacaactgat acggaccggt gccgaccggt tgacggtcga gttgctcctg
    19321 gcgatcttct ttctctaact tccctgcata ttctgccgac atgaccgaag cataatgggt
    19381 cgcgaggtgc cacaaaaaag aagcatccgg ctgagccaga cggaactcaa cggtatgatt
    19441 atccagtttg cggacgcttt tcacgttatc ggcaaattgc aggctgtcga agtaggggaa
    19501 gttgctgcca ttaacgttat gccacgggtt gttgcggtca aaaattcgct ggaaggtaaa
    19561 aaccacatcg tcggcattca ttttacgagt gggagtaaac cagtcggttt tttgaaacgg
    19621 aacatcgcga cgcaggtgga agcgataggt tgcgccgttg tcgagtactt cccagctttc
    19681 ggcaagttcc ggcatcagac gataggtata gggatcgaca tccagcagtc gatcataaaa
    19741 ctgggcggca agggtatcga caattaaccc actgctcgct ttggatgggt taaaggtgtt
    19801 gacttgcccg ctgacgcaat agacaaaacc gctgtcgcgg atatcagcat gcggggggga
    19861 ttcaggcgcg gcgattgcct gaccactcac aagtccagca atcaccaaaa gagacgataa
    19921 tacctggcgc ataatattaa gggattttat gtaaagaggc tatcttacta atatttaatg
    19981 acatttgcca ttaccgtttg tgttcagagg taataatgag ggccgtgtgg ttggtcattg
    20041 aacaggtggc gtcgccatca gttcaacatt cgtacccgtg atttctttat aactataact
    20101 cacagaacaa cttagcgagg agcagagcgg tacgatcgtg agaaacccat taaagcacgc
    20161 tgcagtgcgt gtcattttta gccggatgcg gcgcgaacgc tttatccggg caacgatact
    20221 gaccgatagt ctgcaattaa ataattactc attaccccat tgattcaaaa actctgcgat
    20281 ctcatcaatg cgtacgggat taatccccgc ttcagcagcc atttcatgtt gggcttcttc
    20341 actgatctct tcattgttca tcaaacgggt gagcagtaac tggaagtagt gggccagcgg
    20401 tgtgcgttca gcgtctgcga caggctctgc gcattcggtc gcgtactcat cggcaatatc
    20461 ataatatttt agcgggatat cgtggttcat tatttgcccc tggggttaat gcgctaagca
    20521 taagggcgga tgatagcact cgtgatccgg gtcatacata tttttatagt taatggcgcg
    20581 gtgcattacc ctcaggcagg ataatgcgcc gcgcattcga ctattacgtt tcgctcaccg
    20641 gcgttctgtc gccatcatac aatggcactt tgcgatagcg gcggatcaaa taccacaaat
    20701 atgcgccgcc taacgaagcc cataccatcc cggttgcgca attcgatgcg gcaatgaaaa
    20761 ttttgcagga tgcgctgaga gattaatacg cagaagcagg gttaacgcaa taatgcctga
    20821 tgcgcagcgc ttatcaggcc tgcaaacgac gtattgattt tgtaggcctg ataaggcgtt
    20881 cacggcacac ccggcaagtt gcattgtcga aatgctaaag ctggtgcttt ttcagcaacg
    20941 cgcggaactg atgataggtt aaccccagca attcagcggc gcgcttctgg ttatacttcc
    21001 cttgttccaa gctgacctga agcagttctt tttcctgctg catctgaaac tcgcgtaaat
    21061 ccagcggcag cgtcggaagc gaggtgtttt ctgaaacggc gatggcttct tcagatggac
    21121 ggcgtttaaa gggatcaata atgatgtcat caagcggata atcgctggtg ccgtggcgat
    21181 acactgaacg ttccaccacg tttttcaatt cacgtatatt tcccggccag cgataattca
    21241 gcaatgtttc tctggcgtgc tcggtaaaac ccgggaacag aggcagcttg atttcccgac
    21301 acatctggat ggcaaagtgt tctgccatca acataatgtc gctttcacgt tcgcgtagtg
    21361 gcggcagttg cacaacatcg aacgctagtc ggtcgagcag gtcagcgcga aaagtccctt
    21421 cattgaccat tgccgggaga tcggcattcg tcgcgcatac caaccgcaca ttcacctgca
    21481 atggctggct gccgccgacg cgctccagtt caccgtactc aatcacacgc aataattttt
    21541 cctgcaccat catcggtgcc gtagcgagtt catcaagaaa tagcgtaccg ccgtcggcac
    21601 gttcaaatct ccctggatga cgtttttgcg caccggtaaa cgcccccgct tcgtgaccaa
    21661 acagttcgga atccagcaaa ttttcattta acgccgcgca gttaagggaa ataaacggcc
    21721 cttgccaacg ggaggagaga taatgcagac ggctggcaat cagctcttta ccggtgccgc
    21781 gctcgccgat gatgagcacc ggtttgtcca gcggtgcgag atgcgaaacc tgttccagca
    21841 cttcgagaaa gctgttcgcc tcaccgagta aattatcttt gtattctgcc atgatgaaat
    21901 tcgccacttg ttagtgtaat tcgctaattc atcctggcat gttgctgttg attcttcaat
    21961 cagatcttta taaatcaaaa agataaaaaa ttggcacgca aattgtatta acagttcagc
    22021 aggacaatcc tgaacgcaga aatcaagagg acaacattat gggtattttt tctcgctttg
    22081 ccgacatcgt gaatgccaac atcaacgctc tgttagagaa agcggaagat ccacagaaac
    22141 tggtgcgtct gatgatccag gagatggaag atacattggt tgaagtacgt tctacttcgg
    22201 cgcgtgcgct ggcagaaaag aaacagctga ctcgccgtat tgaacaagcg tcggctcgtg
    22261 aaattgaatg gcaggaaaaa gcggaactgg cactgctgaa agagagagag gatctggcgc
    22321 gtgcggcgtt aattgaaaaa cagaaactga ccgatctgat taagagcctg gaacatgaag
    22381 tgacgctggt ggacgatacg ctagtgcgta tgaagaaaga gatcggcgag ctggaaaaca
    22441 aattgagcga aacacgcgct cgccagcagg cattgatgtt acgccatcaa gcggcaaact
    22501 cgtcgcgcga tgtgcgtcgt cagctggaca gtggcaaact ggatgaagca atggctcgtt
    22561 tcgaatcttt cgaacgccgt atcgaccaga tggaagcgga agcagaaagc cacagcttcg
    22621 gtaaacaaaa atcgctggac gatcagtttg ccgaactgaa agccgatgat gcaatcagcg
    22681 agcaactggc acaattaaaa gccaaaatga agcaagacaa tcaataataa catccaggcg
    22741 gcgtccgaac gcgccgccgc tcatcgtcta aggagtactt atgagcgcgc tatttctggc
    22801 tattccgtta accatttttg tgctgtttgt tttaccgatc tggttatggc tgcattacag
    22861 caatcgttcc ggtcgcagtg aattgtcaca aagtgagcag cagcgattag cgcaactggc
    22921 tgatgaagca aaacggatgc gcgagcgtat tcaggcgctg gaatccattc ttgatgcaga
    22981 acatccgaac tggagggatc gctaatggcg ggcattaatc tcaataaaaa attatggcgt
    23041 attccgcagc agggcatggt ccgtggcgtc tgcgccggga ttgccaacta ttttgatgta
    23101 ccagtaaaac tggtgcgtat cctggtggtg ctgtcgattt tcttcggtct ggcgctgttt
    23161 accctggttg cttacatcat tttgtcattt gcgcttgatc caatgccgga caacatggcc
    23221 tttggtgagc agctaccttc cagcagcgaa ttgctggatg aagtcgaccg tgaactggcg
    23281 gcaagtgaaa cgcgtttacg cgagatggaa cgttatgtca cttccgacac tttcacgctg
    23341 cgtagccgtt tccgtcaact gtgaggaaag ttatgaatac tcgctggcaa caggccgggc
    23401 aaaaggtgaa gcctgggttc aaattagcag gcaagctggt acttcttacc gcactgcgct
    23461 atggcccggc gggtgtggcg ggctgggcga taaaatcagt tgctcgccga ccgctgaaaa
    23521 tgttgctggc tgtggcgctg gaaccgctgt taagtcgggc tgccaataaa ctggcgcagc
    23581 gttataaaag gtgaggggag tttcgcaaaa atggttaaat ctcaggcgta taatggatag
    23641 caattttcat ccatagaagg acgcttacat gtttaaaaaa ggcttacttg ctctgacact
    23701 ggtgttttca ctgcccgttt tcgccgctga acactggatc gatgttcgtg ttccagagca
    23761 gtatcagcaa gagcacgttc agggggccat caatattccc ctgaaggaag tgaaagagcg
    23821 cattgctacc gccgttccgg ataaaaacga caccgtgaaa gtgtattgca atgccggacg
    23881 ccagtcaggg caggcaaaag agatccttag cgagatggga tatacccacg ttgagaacgc
    23941 cggtggcctg aaagacatcg caatgccgaa ggtcaaaggt taaaagaatt caccatgagc
    24001 ggcgcttatg cgccgttttt ttctgtcaca ccttatttac atccccatag attatttgcg
    24061 tcagctcaca aatacgcttt ttcccaggta aaaaaagatt tcctgcgtga ctaaacccct
    24121 tgagctcaat tgacagttta ttttatgcgg agtagtctct cgcttcatgg gaccgctacc
    24181 acggaaaggc aacatgcaac agaaaattac ggattacctg gacgaaatct acggtggaac
    24241 atttaccgcg actcatttac agaaacttgt aacgcgtctt gagagtgcga aacgattaat
    24301 tacacagcga cgtaaaaaac actgggatga aagtgatgtc gtgttaatta cctatgccga
    24361 tcaatttcac agtaatgatt taaagccact acccacattt aatcagtttt accctcaatg
    24421 gctgcaaaga attttttcac atgttcattt attacctttt tatccatggt catctgatga
    24481 tggattttcg gtaattgatt atcatcaggt cgccagtgaa gcgggggagt ggcaggatat
    24541 tcagcaactc ggtgaatgca gccatttaat gtttgatttt gtctgcaacc atatgtcggc
    24601 aaaaagtgaa tggtttaaaa actatttaca acagcagcca ggttttgaag atttttttat
    24661 taccgttgat ccgaaaaccg atctcagcgc cgtcactcgc ccgcgtgcgt taccgttatt
    24721 aacgccattc cagatgcgcg atcattcaac gcgtcattta tggaccacct ttagcgacga
    24781 tcaaattgac ctcaattacc gtagccctga agtgttgctg gcgatggtgg atgtattgct
    24841 gtgctacctg gaaaagggcg cagagtatgt ccgtctggat gccgttggct ttatgtggaa
    24901 agagccggga acaaactgca tccatctgga aaaaacacat ctgattatca aactgttacg
    24961 ttcgattatt gataacgtcg cgccaggcac agtgatcatt accgagacca atgttccgca
    25021 taaagacaac attgcttact ttggcgaagg cgatgacgaa gcgcatatgg tgtaccagtt
    25081 ctcgctgccg ccgctggtgc tgcatgcggt gcaaaaacag aacgttgagg cactttgtgc
    25141 gtgggcgcaa aacctgacac taccttccag caacaccacc tggtttaact tccttgcctc
    25201 tcacgatggc atcgggctta acccactgcg ggggttgtta cccgaaaacg aaatattagc
    25261 gctggtcgag gtgttacagc aggaaggggc attagtaaac tggaaaaata atcccgacgg
    25321 tacgcgtagc ccctatgaaa tgaatgtcac ctatatggat gcgttgagcc gccgtgagtg
    25381 tagcgacgaa gaacggtgtg ccaggtttat tctcgcccat gcgattttgt taagtttccc
    25441 cggtgtgcca gcgatatata ttcaaagcat tctggggtcg cgtaatgatt acgccggtgt
    25501 cgaaaaactt ggatataatc gcgcgattaa ccgtaaaaaa tactctagta aagagataac
    25561 gacagaactg gacaataaag cgacattaag gtattcggta tattataagt tgtcgcattt
    25621 aattaccctt cgtcgcagtc ataaagagtt tcatccggat aataatttca ccatcgatgc
    25681 aattaattca tttgtaatgc gaattcaaag aagcaatgcg gatggcgatt gcctgacggg
    25741 attgtttaat gtcagcgaaa atattcagca tataaatatt actgacctgc acggtcggga
    25801 tctgattagt gaagttgata tattgggtaa tgaaataaca ctgcatccct ggcaggttat
    25861 gtggattaaa taaaaaggaa catttcatga ttaaatcaaa aatcgtgctg ttatcagcac
    25921 tggtttcctg cgccctgatt tcaggctgta aagaagaaaa taaaaataat gtatccatcg
    25981 aatttatgca ttcttcggtg gagcaggagc gccaggccgt catcagcaaa ttgattgccc
    26041 gttttgaaaa agaaaaccca ggtatcaccg taaaacaagt gcccgtggaa gaagacgcct
    26101 ataacactaa agtcattacc ctttcacgta gtggttcgct gccggaagtg atcgaaacca
    26161 gccatgacta cgccaaagtg atggacaaag agcagcttat cgatcgccag gcggttgcca
    26221 cggtcatcag caacgtcggt gaaggcgcgt tctacgatgg cgtactgcgg attgtgcgca
    26281 ccgaagatgg cagcgcctgg accggtgttc ctgtcagcgc atggattggc gggatctggt
    26341 atcgcaaaga tgtgctggcg aaagctgggc tggaggagcc aaaaaactgg cagcaattgc
    26401 tggacgttgc acagaaactg aatgacccgg cgaataaaaa atacggtatt gcgctgccta
    26461 cagcagaaag cgtgttgacg gaacagtctt tctcccagtt tgcgttatcc aatcaggcta
    26521 acgtctttaa cgccgaaggc aaaatcaccc ttgatacacc agagatgatg caggcgctga
    26581 cctattaccg cgaccttgct gccaacacga tgccaggttc taacgacatc atggaagtga
    26641 aagacgcctt tatgaacggc accgcgccga tggcgattta ctccacctat atccttccgg
    26701 ctgtgattaa agaaggcgat ccgaaaaacg tcggtttcgt ggtgccaacc gagaaaaatt
    26761 ctgcggtcta cggcatgttg acctcgctga ccattaccgc cgggcaaaag gccgaagaga
    26821 cggaagcagc agaaaaattt gtcaccttta tggagcaggc ggacaacatt gccgactggg
    26881 tgatgatgtc gccaggcgcg gcgctgccgg tgaacaaagc ggtggtgact accgccacct
    26941 ggaaagacaa cgacgttatt aaggcgctgg gagaattgcc gaatcagcta atcagcgaac
    27001 tgccaaatat tcaggtcttt ggcgcagtag gggataaaaa ctttacccgc atgggcgatg
    27061 tgacggggtc tggcgtggtg agttcaatgg tgcataacgt caccgtgggt aacgccgatc
    27121 tccctggcac gctgcaagcg agccagaaaa agctggatga actgatcgaa cagcactaaa
    27181 cccaggacag gaatccgcaa tgaacaggct tttttcaggt cgttccgata tgccttttgc
    27241 actgctgctt ctcgcgccca gcttattact gctgggcggt ctggtggcgt ggccgatggt
    27301 gtcgaatatc gaaatcagtt ttttacgtct gccgctcaat cccaacatcg agtcaacctt
    27361 tgttggggtg agtaactatg tgcgcattct ctccgatcct ggtttctggc actcgctgtg
    27421 gatgacggtc tggtataccg cgcttgtggt ggcgggcagc accgtgctcg ggctggccgt
    27481 ggcgatgttt ttcaaccgcg agttccgcct gcgcaaaacc gcgcgttcgc tggtgatcct
    27541 ttcctacgta acgccgtcta tttcgctggt gttcgcctgg aaatacatgt tcaacaacgg
    27601 ctacggcatt gtgaactacc tcggcgtcga tcttctgcat ctctatgagc aggcaccgct
    27661 gtggttcgat aatccgggca gtagctttgt gctggtggtg ctgtttgcca tctggcgcta
    27721 cttcccgtat gcctttatct cgtttctggc gattttgcag accattgata aatcgttgta
    27781 cgaagcggca gagatggatg gcgctaatgc ctggcagcgg tttcgcattg tcacgctgcc
    27841 cgcgattatg ccggtcctgg cgacggtggt gacgctgcgc actatctgga tgttctacat
    27901 gttcgcggat gtttatctgc tgacgaccaa ggtcgatatt ctcggtgtct atctctacaa
    27961 aaccgccttt gcctttaatg acttagggaa agcggcggcg atctcggtgg tgctcttcat
    28021 cattattttc gctgtcattc tgctgaccag gaaacgggtg aatctcaatg gcaacaaata
    28081 aacgcacact cagtcgaatc ggtttttact gcgggctggc gctgtttctc atcatcacgc
    28141 tgtttccgtt ttttgtgatg ctgatgacct cgttcaagag cgcaaaagag gcgatctcgc
    28201 tgcatcctac gctgctgcca cagcagtgga cgctggagca ttacgtcgac atttttaatc
    28261 cggtgatttt cccgtttgtc gactacttcc gtaacagcat ggtggtgtcg gtggtttcat
    28321 ccgtggtggc ggtatttctc ggcattcttg gggcttacgc gctttcccgc ctgcgcttta
    28381 aagggcgaat gaccatcaac gccagctttt acacggtgta catgttctcc ggcattttgc
    28441 tggtggtgcc gctgttcaaa atcatcaccg cacttggcat ttacgacacc gagatggcgc
    28501 tgattatcac catggtgacg caaactctgc ctaccgccgt gtttatgctg aaaagctact
    28561 tcgacaccat tcctgatgag atcgaagaag cggcgatgat ggatggcctc aatcgtctgc
    28621 aaattatctt ccgtatcacc gtgcccctgg caatgtccgg gctgatatcc gtattcgtct
    28681 actgctttat ggtggcgtgg aacgactatc tgtttgcgtc gattttcctt tccagcgcca
    28741 gcaatttcac cttacctgtg ggcctgaacg cgctgttcag tacgcctgat tacatctggg
    28801 gacggatgat ggcggcctcg ctggtgaccg cactaccggt agtgattatg tatgcgcttt
    28861 cggaacgttt tattaagagt ggtttgaccg ctggtggcgt gaagggctaa agcggccagt
    28921 ttctttacaa ggagttttac atgaaaaagt tagtagccac agcaccgcga gttgctgcgc
    28981 tggttgagta tgaagatcgg gcgattttag ccaatgaagt gaagatccgc gtgcgtttcg
    29041 gcgctccgaa gcacgggacg gaagtggtcg atttccgcgc tgccagtccg tttatcgatg
    29101 aagactttaa cggtgaatgg cagatgttca gcccgcgtcc ggcaaatgcg ccgcgcggca
    29161 ttgagtttgg caagttccag cttggcaaca tggtggttgg cgacattatc gagtgcggca
    29221 gcgacgttaa cgactacgcg gtgggcgaca gcgtatgcgg ctacggcccg ctttccgaga
    29281 cggtcatcat taacgcagtg aataactaca agctgcgcaa aatgccgcaa ggcagctcct
    29341 ggaaaaacgc cgtctgctac gacccggcgc agtttgccat gagcggcgtg cgcgatgcca
    29401 acgtacgcgt gggcgatttt gtggtggtgg tcgggcttgg cgcgatcggt caaattgcca
    29461 tccaactggc aaaacgcgct ggagcgtcgg tggtaattgg cgtcgatcct attacccgtc
    29521 gttgtgatat tgcccgtcgc cacggtgcgg atttctgtct taaccccatt ggcgctgacg
    29581 taggtaaaga gatcaaaacg ctgaccggca agcagggtgc cgatgtgatt atcgaaacca
    29641 gcggttacgc cgacgcgctg caatcggcgc tccgcggtct ggcctacggc ggcaccatct
    29701 cctatgtcgc gtttgccaaa ccatttgctg aaggttttaa cctcggacgc gaagcgcatt
    29761 tcaataacgc caaaattgtt ttctcccgcg cgtgcagcga gccgaacccg gattatccgc
    29821 gctggagccg taagcgtatt gaagaaacct gctgggaact gctgatgaac ggttatctca
    29881 attgcgaaga tttaatcgat ccggtggtga cctttgccaa cagcccggaa agctacatgc
    29941 agtatgtcga ccagcatccg gaacagagca tcaaaatggg cgtcacgttt taattcaaag
    30001 gaatgataaa aatgaaaatc ggcacacaga atcaggcgtt ctttccggaa aacattctgg
    30061 agaaatttcg ttatatcaaa gagatgggtt tcgatggttt tgagattgac ggcaaactgc
    30121 tggtcaacaa tctcgaagaa gtcaaagcag caatcaaaga aaccggttta ccggtgacca
    30181 ccgcctgcgg tggctatgac ggctggattg gcgactttat cgaagagcgt cgacttaatg
    30241 gcttaaagca gatcgaacgc attcttgaag cgctggcaga agtgggcggt aaaggtatcg
    30301 tcgttccggc tgcatggggc atgtttacct tccgcttacc gccgatgacc tcgccgcgta
    30361 gcctggacgg cgaccgcaaa atggtgagtg attccctgcg cgtactggaa caggcggccg
    30421 cacgtaccgg aaccgtggtg tacctcgaac cgttaaaccg ctatcaggat cacatgatca
    30481 acaccctcgc cgatgctcgt cgttacatcg tcgaaaacga tcttaaacat gtacagatta
    30541 tcggcgattt ctatcacatg aatatcgaag aagataacct ggcgcaggcg ttgcacgaca
    30601 accgcgactt gctcggtcat gtgcatattg ccgataacca tcgctaccag ccgggcagcg
    30661 gcaccctcga tttccacgcg ctgtttgaac agctgcgcgc cgataactat cagggttatg
    30721 tggtgtatga agggcgtatc cgggcagaag ctcctgccca ggcgtaccgt gattcgttgg
    30781 cctggttgcg cacctgctaa gaggtctttg tgaaaagtgc aatgacaagc tctccgctgc
    30841 gtgtcgcgat aatcggcgca ggccaggtgg cggataaagt tcatgcttcg tactactgca
    30901 cccgcaacga tctggaactg gtggctgtct gtgacagccg cctttcccag gcgcaggcgc
    30961 tggcagaaaa atacgggaat gcatccgtgt gggacgatcc gcaggccatg ctgctggcgg
    31021 tgaaacctga tgtggttagc gtctgctcac ctaaccgttt tcattacgaa cataccctga
    31081 tggcgctgga agcgggctgc catgtgatgt gcgaaaaacc gcccgccatg acggcagaac
    31141 aggcgcggga aatgtgcgat accgcgcgca aacagggcaa ggtgctggcc tacgactttc
    31201 accatcgttt tgcactcgat acgcaacagc tgcgtgaaca ggtgaccaac ggcgttttgg
    31261 gagagattta cgttaccacc gcccgcgccc tgcgtcgttg cggtgttccc ggctggggtg
    31321 tctttactaa taaagaattg cagggcggcg gcccgctgat cgacatcggc attcatatgc
    31381 tggatgcggc gatgtatgtg ctgggttttc cagcggtgaa aagcgtgact gcgcatagct
    31441 ttcaaaagat cggcacgcaa aagagctgtg gtcaatttgg cgagtgggac ccggcaactt
    31501 acagcgttga agattcgctg tttggcacca ttgaatttca taacggcggc attctgtggc
    31561 tggaaacgtc atttgcactc aacatccgcg aacagtcgat tatgaatgtc agcttttgtg
    31621 gtgataaagc cggtgcgacg ctgtttccgg cacatatcta caccgataac aacggtgaat
    31681 taatgacgct gatgcaacgg gaaatggcag acgacaaccg ccatttgcgc agcatggaag
    31741 cctttatcaa tcacgtacag ggcaagcccg tgatgatagc cgacgccgag caggggtaca
    31801 tcatccagca actggtggcg gcgttgtatc aatccgcgga aacagggacg cgtgtggaat
    31861 tatgaccagg ccagtaacgt tatcagaacc ccatttcagc cagcataccc tgaacaagta
    31921 tgcttcgctg atggcgcagg ggaacggcta tcttgggctt cgtgccagcc atgaagaaga
    31981 ttacacgcgc cagacccgtg ggatgtatct ggcggggctg tatcatcgag cgggtaaagg
    32041 agaaatcaac gaactggtga acctgcctga tgtcgtgggg atggagattg ccataaatgg
    32101 tgagattttc tcgttatccc gcgaagcatg gcagcgtgag cttaactttg ccagtggcga
    32161 attacgtcgc aacgttgtct ggcgtaccag caacggcact ggttacacca tcgccagccg
    32221 tcgctttgtt tcggcagacc aactgccgct cattgcgctg gaaatcacta ttacgccact
    32281 ggacgccgac gcgtcagtgc tgatttcaac aggcattgac gccacgcaaa ccaaccacgg
    32341 tcgccagcat attgacgaaa tccaggtgcg ggtgtttggt cagcatctga tgcaggggat
    32401 ctacaccacc caggatgggc gtagtgatgt ggccatcagc tgttgctgta aggtgagcgg
    32461 tgatgtgcag caatgctata ccgccaaaga gcgccgttta ttgcaacata cctgcgcgca
    32521 gcttcacgca ggtgagacgc tgacgctgca aaaactggtg tggatcgact ggcgggacga
    32581 cagacaagcc gctttagacg aatggggcag tgcgtcgctt cgtcagcttg aaatgtgcgc
    32641 gcagcagagt tacggccaac ttcttgcagc atcaacagaa aactggcgtc aatggtggca
    32701 gaaacgtcgt atcacggtaa acggcggcga tgcgcacgat cagcaagcat tagattatgc
    32761 gctttatcac ctgcgcatca tgacgcctgc ccacgatgag cgcagcagca ttgcggcgaa
    32821 aggtttaacc ggcgaaggct acaaaggcca cgttttctgg gatacagaag tatttttgct
    32881 accgttccat ctgtttagcg atccgacggt tgcccgaagt ttactgcgtt atcgctggca
    32941 caacttgcct ggcgcgcagg agaaagcgcg acgcaacggc tggcagggcg ctctgtttcc
    33001 gtgggaaagc gcgcgtagcg gcgaagaaga gacgccggaa tttgccgcca ttaacattcg
    33061 taccgggctg cggcaaaaag tggcctctgc gcaggcggaa caccatctgg tcgccgatat
    33121 cgcctgggcg gttattcaat actggcggac cacgggggat gaaagtttca ttgctcatga
    33181 aggcatggcg ctacttctgg agacggcaaa gttttggatt agccgcgcgg tgagagttaa
    33241 cgatcgtctg gaaattcatg atgttattgg gccagacgaa tataccgaac atgtcaataa
    33301 taacgccttc accagctata tggcgtatta caacgtccag caggcgctga atatcgctcg
    33361 tcagtttggc tgtagcgacg acgcgtttat tcatcgtgca gaaatgtacc tcaaagaact
    33421 gcggctgcca gaaattcagc ccgacggcgt tttgccgcag gatgattcgt ttatggctaa
    33481 gccgttgatt aatctggcga aatacaaagt ggcggcgggg aagcaaacca ttctgctgga
    33541 ttattcacgc gcagaagtga acgagatgca gatcctcaaa caagctgatg tggtgatgct
    33601 caattacatg ctgccggagc agttctcagc ggcatcgtgt cttgccaatc tgcaatttta
    33661 tgaaccgcgc actattcacg actcgtcatt aagtaaagca atccacggca ttgttgccgc
    33721 acgctgtggc ctgctgaccc aaagttatca gttctggcgc gaggggactg aaatcgatct
    33781 tggtgctgat ccacctagct gtgatgacgg tatccacgct gccgcaactg gcgctatctg
    33841 gctgggggcg attcagggtt ttgccggggt gagtgtgcgt gacggtgaat tacatctcaa
    33901 tccggcgtta cctgagcagt ggcaacagtt gtctttccct ctgttctggc agggctgcga
    33961 attacaggtc actctcgacg cgcagcgtat tgcgattcga acttctgcgc ctgtttcact
    34021 gcgcttgaac ggtcagctta tttacgtcgc tgaagaatct gttttctgtt tgggggattt
    34081 tattttgccc ttcaatggga ccgctaccac gcatcaggag ggtgaatgaa actgcaaggg
    34141 gtaattttcg atctggatgg agtaatcacc gataccgcgc atctgcattt ccaggcgtgg
    34201 cagcagattg ccgctgaaat tggcatcagc attgatgcgc agtttaacga attcctaaaa
    34261 gggatcagcc gcgatgagtc cctgtggcga attctgcagc acgggggtaa agagggcgat
    34321 tttaacccac aggaacgagc gcaactggcg tatcgcaaaa atctgcttta cgtccactca
    34381 ctgcacgaac tgacggttaa tgctgtgctg cctggcattc acaatttgct ggtagatctc
    34441 cgggcacagc agattccggt tgggctggct tctgtctccc tgaatgcgcc gacgatttta
    34501 gcagcgctgg agctgcgcga gtttttcacc ttctgcgcgg acgcttccca actcaaaaat
    34561 tcgaaaccgg acccggaaat ctttctcgcc gcctgtgcag ggctgggcgt gccgccacag
    34621 gcatgtatcg gcattgaaga tgcgcaggcg ggcattgaag ccattaacgc cagcggtatg
    34681 cgctcggtgg ggatcggcgc gggcttaacc ggggcgcaat tattgttgcc ctcaacggac
    34741 tccctgacct ggccgcggtt atcggccttc tggcaaaacg tatagcaaag gaatcaacat
    34801 ggcacagctt tcgttacaac atattcaaaa gatctacgat aaccaggtgc atgtggtgaa
    34861 ggacttcagc ctggagattg ccgataaaga gttcatcgtg tttgtcggcc cgtcgggctg
    34921 cggtaaatcg accaccctgc gtatgattgc cgggcttgag gagatcagcg gcggtgatct
    34981 gttgatcgac ggcaaacgaa tgaatgacgt tccagccaaa gcacgcaata tcgcgatggt
    35041 gttccagaac tacgcgctgt atccgcatat gacggtctac gacaacatgg cgtttggcct
    35101 gaagatgcaa aaaatcgcca aagaggtgat tgatgagcgg gtgaactggg cggcgcaaat
    35161 tctcggcctg cgtgagtacc tgaaacgcaa gccgggcgcg ctttccggcg ggcaacgtca
    35221 gcgagtggcg cttgggcggg cgattgtgcg cgaagcgggc gtgtttttaa tggatgagcc
    35281 gctctctaac ctggatgcca agctgcgcgt gcaaatgcgc gcagagatta gcaagctgca
    35341 tcagaaactg aacaccacca tgatctacgt gacccacgat cagaccgaag cgatgaccat
    35401 ggcgacgcgg attgtcatta tgaaggatgg gattgttcag caggtcggtg cgccgaaaac
    35461 ggtctataac caaccggcga atatgtttgt tgccggattt attgggtcac cagcaatgaa
    35521 ttttattcgc ggcacgatcg atggcgataa attcgttacg gaaacgctta aattaaccat
    35581 acccgaagag aaattagcgg ttctgaaaac gcaggaaagt ttacataaac ccattgtgat
    35641 gggaatacgc ccggaggata ttcatccgga tgcgcaagaa gaaaataaca tttccgccaa
    35701 aattagcgtg gcggaattaa ccggtgcgga atttatgctc tacaccacgg ttggggggca
    35761 cgagttagtg gtccgtgctg gtgcgttaaa tgattatcat gcaggagaaa atatcactat
    35821 tcattttgat atgactaaat gtcatttctt tgatgcagaa acggaaatag caatttgcta
    35881 aatacagggg gaaggcattc tcccaggata atacaaggaa caataatgaa aaagttatta
    35941 ccctgtaccg cactggtgat gtgtgcggga atggtctgcg cacaggccga ggaaaagaac
    36001 gactggcact ttaatatcgg cgcgatgtac gaaatagaaa acgtcgaggg ttatggcgaa
    36061 gatatggatg ggctggcgga gccttctgtc tattttaatg ccgccaacgg gccgtggaga
    36121 atttctctgg cctattatca ggaagggccg gtagattata gcgcgggtaa acgtggaacg
    36181 tggtttgatc gcccggagct ggaggtacat tatcagttcc tcgaaagcga tgatttcagt
    36241 ttcggtctga ctggtggttt ccgtaactat ggttatcact acgttgatga accgggtaaa
    36301 gacacggcaa atatgcagcg ctggaaaatc gcgccagact gggatgtgaa actgactgac
    36361 gatttacgtt tcaacggttg gctgtcgatg tataaatttg ccaacgatct gaatacgacc
    36421 ggttatgctg atacccgtgt cgaaacggaa acgggtctgc aatatacctt caacgaaacg
    36481 gttgccttgc gagtgaacta ttatctcgag cgtggtttca atatggatga tagccgcaat
    36541 aacggtgagt tttccacgca agaaattcgc gcctatttgc cgctgacgct cggcaaccac
    36601 tcggtgacgc cgtatacgcg cattgggctg gatcgctgga gtaactggga ctggcaggat
    36661 gatattgaac gtgaaggcca tgattttaac cgtgtgggtt tattttacgg ttatgatttc
    36721 cagaatggtc tttccgtttc gctggaatac gcgtttgagt ggcaggatca cgacgaaggc
    36781 gacagcgata aattccatta tgcgggtgtt ggggtgaatt actcgttctg ataatgtgct
    36841 aaattgccgg atgcggggcg agcgccaaat ccggcataca tttatttcaa cgacatatgc
    36901 gtagcaacgg taatattttg tggtgacggt tttccggcaa ttaactggaa taataactcg
    36961 cagctttgtt gacctaactc ctgcgtcgga acatcgatgc cgcctggtgc aggcgttaat
    37021 ataaacgaca gcgtttcatt actataaccc accaccgcta actgctgcgg aatagcaatg
    37081 tttttctctg ccgccgcacg ataaatactc attaatttca ggctgtcagt agcaaacact
    37141 gcctcaggca acggtgactg gcttaataat tcccgtgctg cttttaatgc tgtctcatgg
    37201 gtataaccgc cgtcaacaat ccattcatca cgcactgcaa tattatgcgt agccagactt
    37261 tgcttataac catttacccg atccactgaa acttgtacat caagcggcgc atgcaggcag
    37321 gctatattct tatgcccgct ttcaattaac gcattggtca gcgcaatact gtcgccataa
    37381 ttatcggtat cgacggaata aacatggctg tattgacctt caactttacc gatcaccacc
    37441 accgggatct cgtatttgtc gagttgtgca aagaaggatt catcggcagg cgaacttagc
    37501 ataataatgc ctttaatcat tttctgctta attttgcttt cgcatttttg cagatcttct
    37561 gccggattgt gcgacgtttg taatatcacg tcgaaacctt cttcttctgc tttggcggtg
    37621 atggcatgca aaacttcaga gaaaaatgga ttacccgccg tagttttggt cgaacgagta
    37681 gaaatcacca taatggcatc aaaacccgaa gaggtcagcg cgcgggccag cttgtttggt
    37741 tgatactgta attcttcaat ggcccgtaac actttttcgc gcgcttccgg ggagatattg
    37801 gtttgcttat tcagcacgcg tgatacggtg gattttgata cgcctgcaac cctggcaata
    37861 tcataaatag taggggacat aggtcaggaa ctccgtccga acgtgaatgc tgcacatctt
    37921 atggagttcg cccgtcgatg acaacacggg tagttgataa tcaatggcct ggcccccaca
    37981 ttcatatcct tacgaacgat tttttttctg gtcttcagag cagcacagga cagcaatgaa
    38041 gcgacttaaa aatgaactca atgcgctggt gaatcggggt gtcgacagac atctgcgcct
    38101 cgctgtaacc ggacttagcc gcagcggcaa aacagcgttt atcaccgcga tggttaatca
    38161 gttgctcaat attcatgccg gagcacgttt gccgctttta agtgcggtgc gtgaagagcg
    38221 cctgctgggc gtaaaacgca ttccccagcg tgactttggc attccgcgct tcacctatga
    38281 tgaagggttg gcgcagttat atggcgatcc tcccgcctgg ccgacgccta cgcgcggtgt
    38341 cagtgaaatt cgcctggcgt tacgttttaa atcgaacgat tcgctgctgc gccactttaa
    38401 ggatacctcc acgctgtatc tggagattgt ggattacccc ggcgaatggt tgctcgacct
    38461 gccgatgctg gcgcaggact atttaagctg gtcgcgccag atgacgggct tactcaatgg
    38521 tcagcgcggc gaatggtcgg cgaaatggcg aatgatgtgc gaagggctgg acccgctagc
    38581 gcctgccgac gaaaaccggc tggcagacat tgccgccgcc tggaccgagt atctccatca
    38641 ctgcaaacag caggggttgc actttattca gcctgggcgc tttgtcttgc cgggagatat
    38701 ggcaggagca cccgcgctgc aattcttccc gtggccggat gtcgatgcct ggggcgagtc
    38761 caaactggcg caggccgata agcacactaa tgccggaatg ttgcgcgagc ggtttaatta
    38821 ttactgcgag aaagtggtga aggggttcta taagaatcat tttctgcgct ttgaccgcca
    38881 gattgtgctg gtggattgcc tgcaacctct caacagtggg ccacaggcat ttaatgatat
    38941 gcgtctggcg ctgacgcagc tgatgcaaag tttccactac gggcagcgta ccctgttccg
    39001 gcgtttgttt tcgccggtta tcgataagct attgtttgct gccactaaag cggaccatgt
    39061 gaccatcgat cagcacgcca atatggtttc attgctgcaa caactgattc aggatgcatg
    39121 gcaaaatgcg gcgttcgaag ggatcagtat ggactgcctg gggctggcgt ctgttcaggc
    39181 gaccaccagc ggaatgattg atgttaacgg tgagaaaatc ccggcgctgc gcggtaatcg
    39241 actcagcgat ggcgcaccgc tcactgttta tcctggcgaa gttcccgcac gtttgccagg
    39301 tcaggcgttc tgggataagc aaggcttcca gtttgaggcg tttcgtccgc aggtgatgga
    39361 tgtcgacaaa ccgctaccgc atattcgtct tgatgctgcg ctggaatttt taataggaga
    39421 taaattgcga tgaccgaacc gttaaaacca cgtattgatt tcgacggtcc actggaggtc
    39481 gatcagaatc caaaattcag ggcgcagcag acctttgacg aaaatcaggc gcaaaatttt
    39541 gccccggcca cgctcgacga agcacaggaa gaagaggggc aagttgaagc ggtgatggac
    39601 gcagcgttac gtccgaaacg cagcctgtgg cgcaaaatgg tgatgggcgg gctggctctg
    39661 tttggcgcaa gcgttgtcgg gcagggcgtg cagtggacaa tgaacgcctg gcaaacccag
    39721 gactgggtgg cgctgggtgg atgtgccgct ggggcattga ttatcggcgc tggcgtaggt
    39781 tctgtggtaa cagagtggcg gcgcttatgg cgcttgcgac agcgcgccca tgaacgcgat
    39841 gaagcgcgcg atttgttgca cagtcacggc acgggtaaag gccgtgcatt ctgcgaaaaa
    39901 ctggcgcagc aggcgggcat cgatcagtca catccggcgc tgcagcgctg gtatgcctca
    39961 atccatgaaa cgcagaacga tcgtgaagtg gtcagtttgt atgctcacct ggtccagcca
    40021 gttttagatg cccaggcgcg gcgcgaaatc agccgctcag cggcggaatc aacgttaatg
    40081 attgccgtta gcccactggc gctggtggat atggcgttta tcgcctggcg caatctgcgt
    40141 ttgattaatc gcatcgccac gctgtatggc attgaactgg gatattacag ccgtttgcgc
    40201 ctgtttaagc tggtattgct gaatatcgct ttcgccggag ccagcgaact ggtgcgcgaa
    40261 gtggggatgg actggatgtc gcaagatctc gctgcgcgtt tgtctacccg ggcagctcag
    40321 gggattggtg ctggacttct gacggcacga ctcgggatta aagctatgga gctttgccgc
    40381 ccgctgccgt ggattgacga tgacaagcca cgcctcgggg atttccgtcg tcagcttatc
    40441 ggtcaggtga aagaaactct gcaaaaaggc aaaacgccca gcgaaaaata atgcaatatc
    40501 gggtgctgac cggatatctt tacgccgaag tgcccgtttt tccgtctttg tgtcaatgat
    40561 tgttgacaga aaccttcctg ctatccaaat agtgtcatat catcatatta attgttcttt
    40621 tttcaggtga aggttcccat gcgtctggaa gtcttttgtg aagaccgact cggtctgacc
    40681 cgcgaattac tcgatctact cgtgctaaga ggcattgatt tacgcggtat tgagattgac
    40741 cccattgggc gaatctacct caattttgct gaactggagt ttgagagttt cagcagtctg
    40801 atggccgaaa tacgccgtat tgcaggtgtt accgatgtgc gtactgtccc gtggatgcct
    40861 tccgaacgtg agcatctggc gttgagcgcg ttactggagg cgttgcctga acctgtgctc
    40921 tctgtcgata tgaaaagcaa agtggatatg gcgaacccgg cgagctgtca gctttttggg
    40981 caaaaattgg atcgcctgcg caaccatacc gccgcgcaat tgattaacgg ctttaatttt
    41041 ttacgctggc tggaaagcga accgcaagat tcgcataacg agcatgtcgt tattaatggg
    41101 cagaatttcc tgatggagat tacgcctgtt tatcttcagg atgaaaatga tcaacacgtc
    41161 ctgaccggtg cggtggtgat gctgcgatca acgattcgta tgggccgtca gttgcaaaat
    41221 gtcgccgccc aggatgtcag cgccttcagt caaattgtcg ctgtcagccc gaaaatgaag
    41281 catgttgtcg aacaggcgca gaaactggcg atgctaagcg cgccactgct gattacgggt
    41341 gataccggca ccggaaaaga tctctttgcc tacgcctgtc atcaggcaag ccccagagcg
    41401 agcaaacctt acctggcgct gaactgtgcg tctataccgg aagatgcggt cgagagtgaa
    41461 ctgtttggtc atgctccgga agggaagaaa gggttctttg agcaggcgaa cggtggttcg
    41521 gtgctgttgg atgaaatagg tgagatgtca ccacggatgc aggcgaaatt actgcgtttc
    41581 cttaatgatg gcaccttccg tcgggttggc gaagaccatg aggtgcatgt cgatgtgcgg
    41641 gtgatttgcg ctacgcagaa gaatctggtc gaactggtgc aaaaaggcgt gttccgtgaa
    41701 gatctctatt atcgtctgaa cgtgttgacg ctcaatctgc cgccgctacg tgactgtccg
    41761 caggacatca tgccgttaac cgagctgttc gtcgcccgct ttgccgacga gcagggcgtg
    41821 ccgcgtccga aactggccgc tgatctgaat actgtactta cgcgttatgc gtggccggga
    41881 aatgtgcggc agttaaagaa cgctatctat cgtgcactga cacaactgga cggttatgag
    41941 ctgcgtccac aggatatttt gttgccggat tatgacgccg caacggtagc cgtgggcgaa
    42001 gatgcgatgg aaggttcgct ggacgaaatc accagccgtt ttgaacgctc ggtattaacc
    42061 cagctttatc gcaattatcc cagcacgcgc aaactggcaa aacgtctcgg cgtttcacat
    42121 accgcgattg ccaataagtt gcgggaatat ggtctgagtc agaagaagaa cgaagagtaa
    42181 gcgtgaatat gcctgatggt gcaataccat caggcatatt aacttatgct ttcagtacag
    42241 ccagagctgc ttcgtaatcc ggctcggtgg tgatttcatc caccagctgg ctgaaaatca
    42301 cattgtcatt ttcatcaata accacaacgg cacgcgctgc cagacctttc agtgggccat
    42361 cagcaattgc cacaccgtag gcttgcagga attcagcgtt acggaaagtg gagagggtga
    42421 taacgttgtt cagaccttct gcgccgcaga aacgagactg ggcgaacggc agatcggcag
    42481 agatacacag cacaacggtg ttgtcgatct cggttgccag ttggttaaac ttacgtactg
    42541 atgcggcgca aacaccggta tcaatactcg ggaaaatgtt cagcactttg cgtttacccg
    42601 caaactgacc gagggtgacg tcagacagat cttttgccac gagagtaaaa gtctgcgctt
    42661 tgctacccgc ctgcgggatg gaattggcga ctgtaaccgg gttgccctgg aaatgaacgg
    42721 tttgtgacat gattatcttt cctgtttaca tatagttaac gtcacaccta gtttatgcta
    42781 actgtcaata acacagcaaa cgctatttgc gcttaatccg cagaccaccg cgacaacaag
    42841 gagtaaagat gagaaccgtt aaggtatttg aggaggcctg gcccttacat accccgtttg
    42901 tgattgctcg gggaagtcgc agtgaagcgc gcgtggtggt ggttgaactg gaagaagagg
    42961 gtatcaaagg caccggtgaa tgcacgccgt atccgcgtta tggggaaagt gatgcctcgg
    43021 taatggcgca aattatgagc gtcgtgccgc aactagagaa aggactgaca cgggaggagt
    43081 tgcaaaaaat cctccctgcc ggcgcagcac gtaatgcgct ggattgtgca ttgtgggatc
    43141 tggcggcgcg aaaacagcag caatcgctgg ctgatttgat cggcataacg cttcccgaga
    43201 cagtcaccac ggcacagacg atagtcatcg gtacgcctga tcagatggcc aatagtgcat
    43261 caacactctg gcaggcaggc gcgaaattac tgaaagtgaa gctggataac catcttatca
    43321 gtgagcggat ggtggcaatt cgcacagctg tgcccgatgc gacgctgatc gttgatgcaa
    43381 atgaatcctg gcgtgcagaa gggttggcgg cgcgttgcca gctattggcg gatttaggcg
    43441 ttgcgatgct tgaacaaccg cttcctgcgc aggacgatgc ggcgctggag aattttattc
    43501 atccgttacc gatttgtgct gatgaaagtt gtcatactcg tagcaatctg aaggcgctga
    43561 aagggcgcta tgagatggtt aacattaagc ttgataaaac cggtggtctg acggaagcgc
    43621 tggcgctggc gactgaagcg cgtgcacaag gttttcgtct gatgctgggc tgcatgttgt
    43681 gtacctctcg tgccattagc gccgctttac cgctggtgcc gcaggtcagt ttcgccgatc
    43741 ttgacggacc gacctggctg gcggtagatg tggaaccggc gcttcagttc acgacgggcg
    43801 aattgcatct ttaggatgcc agcgcagcaa gtttgccatc gcaaagagat atttttcgct
    43861 ggcttcgtcg gaggagatgg gtggaaactc tgcggtgatg caatgcaagt tgagatctgc
    43921 acaccaactg ccgaaagagc caggcgtttc ataaccgacg ctggttacca gaggcaattc
    43981 aaacgcctgg gccagccatt cacctaattc gctgtgtctg ggatcttcaa tacaggccag
    44041 cggatcgtgg aatgagacca cccaggcagg ttgaatgcgg tgtataagct ggcacagcgc
    44101 ctgggtttca ggttcggagc caggtttgtc acctgtcagt aatacaacat cacgttcttc
    44161 agcggcgctg ttccagcgat aaactgtttc accttccttc cagttcgctg ccggaaagtt
    44221 tcggtttaaa tccacaccat tagcattggc ccgtaaccct aactggcagc cgtcaggatt
    44281 cacacacagc accacatgat ggcggcgcaa agaaggtgtc aaagtccgca gagcacagga
    44341 gagcgtgacg atcgaagagt tttcatcacc gtgagtgcca gccagaatca aaccactttc
    44401 gtgactggct gcaggggccg gaaaccagat taacggcgcg cccaataatg aacgtccgta
    44461 atgttctgtt ccgggcggaa atgcgccgcg ttcggcgcgt gggcgagtta cggtcatgat
    44521 cgtctctggt tcaggaagta ttacaggcag tgttgtgcaa attttgcgga ttatcaaatc
    44581 cttttcttca acgctatgtc atcgatcttg tatcactttt ttgccggaag ttgtttgcat
    44641 tttcattgat gtgcagatat tagaattgtc gtaatgttat atacatggcg tattttattc
    44701 ttactggttc tacacatgtt aatcgcatct gattgatcag ttcaccttaa taagtttaat
    44761 aaatcgtact gcacatagta cagttcgatg gtagctatat aattgattta catgaagaag
    44821 ggcttatcac catcagtccc gggttgattg atgcctatat tctcaccaat gtgtttctca
    44881 ggtttatcga tgatatctgt gattaatgct gcgacactct tacgtgatac gatcgtacct
    44941 ttgaatggtt cattgcgtga ggtaagctca taatcaataa tatcttcatc agtcagccag
    45001 gcagggcgaa gaattgtata ttccagtcca gaagcctcaa ttgcatccgc agccctgcgg
    45061 aatggtttta ggggttcacc aataactgca ttattccatt cgccaaattt tcctggaact
    45121 tcatcataaa taccgaggga aagaacaaag atcagacgtt taacatcaca ggctttcatc
    45181 gcagcgatca ctgaatttgc ctgaatatct aaatcttccc ccgtaaggtt ggcatacaca
    45241 acatcctgtc cctgcatggc ctgcttcagt gctgcatgat tcagtacatc ccccataata
    45301 atcttgctat tagttggata gggtttgtgg atttttgctg gctgacgggc aaataaagtt
    45361 tgtttaatgg tttgtttatc tgccagttga ttaatcacat ggcgggctat ctgaccgcca
    45421 gcaccaagaa tcagtacatt tttcatgaaa cttttcccag attattggca gcaatatgcc
    45481 aggccatatc actattacgg atttgaatat gtttcattac agagtttcct caaagaacgg
    45541 agctaatact gaaattactt cagccacata gactttgcca tcataaagat ccatatggtt
    45601 tgcgccttca acaatgtgat agcgtttatc ctggcttgat gctcgatcgt acaggtcgtc
    45661 actcatccat ttgctcccag ccaggctgcc cgccacaatc tgtatcggct gagtcaggta
    45721 cacttccgcc atatggtaag catcataggt aataatctgg ttaaggctgc gcaaagtagc
    45781 gtaacccggt gctgttggat actgcgcgcg aggggtgtgg taatattccc aggcctgacg
    45841 cagttcttcg ttcggcgcat cggactcctt cattggtgcc agtggcatag tggcgtattc
    45901 tccgctgcta atatcgctgg ttcgggcgtt tgaacccgcg tcaacgtatg gtagggcatc
    45961 aatagatttc acattgtttt cccaaccatt acggaacatc gaaccaatat tgaccgcact
    46021 aacagtaccg atggccttga tgcggcgatc ctgaattgca gcattggctg tatatcctgc
    46081 accggcacaa attcccatcg caccaattcg ggtattgtcg acataagaaa gcgttgtcag
    46141 gtaatcaatc acggcactga cgtcttcagt acgaatgtat gggttttcta actgacgcgg
    46201 ctcgccgccg ctttcacctt gataagatgc gtcataagca atagtgacaa atcctttttc
    46261 cgccagtttt tcggcatagg ttccggccgt ttgctcttta acgcccccac ctgggtgaga
    46321 taccacaatt gccggatact gacgggtttc atcaaatttt ggagggaaat agatcactgc
    46381 agacaaagag atggttggat tattgctgtt agtgaagctg actttattat tcatcattcc
    46441 gttcctcatg gagttgtcgg ttcgttttaa cggttggtga tatcactata gatattgatc
    46501 attaagttga ttagacccaa aatcatgatt agacctatca tttaaatgat taatagattg
    46561 tatggagata ctgaatgaag cgtgaagaaa tcgctgatct gatggcgttt gtcgttgttg
    46621 cagaggagcg tagcttcact cgcgcagcag cccgcctgag catggcgcag tcagctttaa
    46681 gccagatagt gcgccgtata gaagaacgat tgggattgcg gcttctgacg cgaaccacgc
    46741 gcagcgttgt acccactgaa gcgggcgagc atcttttgtc tgttcttggc cctatgttgc
    46801 atgacataga ttctgccctg acatccctga gcgatctgca gaaccgccca tccgggacaa
    46861 tacgtattac tactgtagaa catgcagcaa aaacgatatt gttaccagcc atgcgcacat
    46921 tcctgaaatc gcatcctgaa attgatattc agcttaccat tgattatggt ttgaccgatg
    46981 tcgtttctga acgttttgat gcaggcgtcc gtctgggtgg ggagatggat aaagatatga
    47041 tcgccattcg aatcgggcca gacataccaa tggctattgt tggctcaccg gattattttt
    47101 ctcgtcgaag tgctccaacg tcagtgtcac aattaataga tcatcaggca attaatttgt
    47161 atcttcccac atcgggtaca gcaaatcgct ggagattaat acgcggtgga cgtgaagttc
    47221 gtgttcgcat ggaaggtcag cttttactga atacgataga tctgatcatt gatgctgcaa
    47281 ttgatgggca tggattggcg tatctacctt atgatcaggt ggagcgggct attaaagaaa
    47341 aaaaactgat acgtgttttg gataaattca caccagattt acccggttat cacctgtact
    47401 atccacaccg tcgacatgct ggctcggcat tctcattatt tatagatagg ctgaagtata
    47461 aaggtgctgt ttagcactac ttgctgatac attaatttaa ttcttctctt aacgtattct
    47521 cagttccttt caacgttttg gttatttttt attcttcgca caatggcgac agatgctgat
    47581 tatgataccg aaaacgggtt tgaatgtgcg aagcccgaac gtagtgttcg gagttctatg
    47641 tgctttaccg cattttggag ac
//
LOCUS       DAHHTG010000021        47506 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.021, whole genome shotgun sequence.
ACCESSION   DAHHTG010000021 DAHHTG010000000
VERSION     DAHHTG010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 47506)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47506)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47506
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.021"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            105..608
                     /gene="dps"
                     /locus_tag="NBI74_001187"
                     /gene_synonym="pexB"
     CDS             105..608
                     /gene="dps"
                     /locus_tag="NBI74_001187"
                     /gene_synonym="pexB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001730644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein Dps"
                     /protein_id="HCD1931552.1"
                     /translation="MSTAKLVKSKATNLLYTRNDVSDSEKKATVELLNRQVIQFIDLS
                     LITKQAHWNMRGANFIAVHEMLDGFRTALIDHLDTMAERAVQLGGVALGTTQVINSKT
                     PLKSYPLDIHNVQDHLKELADRYAIVANDVRKAIGEAKDDDTADILTAASRDLDKFLW
                     FIESNIE"
     gene            1012..1758
                     /gene="glnH"
                     /locus_tag="NBI74_001188"
     CDS             1012..1758
                     /gene="glnH"
                     /locus_tag="NBI74_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter substrate-binding
                     protein GlnH"
                     /protein_id="HCD1931553.1"
                     /translation="MKSVLKVSLAALTLAFAVSSHAADKKLVVATDTAFVPFEFKQGD
                     KYVGFDVDLWAAIAKELKLDYELKPMDFSGIIPALQTKNVDLALAGITITDERKKAID
                     FSDGYYKSGLLVMVKANNNDVKSVKDLDGKVVAVKSGTGSVDYAKANIKTKDLRQFPN
                     IDNAYMELGTNRADAVLHDTPNILYFIKTAGNGQFKAVGDSLEAQQYGIAFPKGSDEL
                     RDKVNGALKTLRENGTYNEIYKKWFGTEPK"
     gene            1897..2556
                     /gene="glnP"
                     /locus_tag="NBI74_001189"
     CDS             1897..2556
                     /gene="glnP"
                     /locus_tag="NBI74_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015367581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter permease GlnP"
                     /protein_id="HCD1931554.1"
                     /translation="MQFDWSAIWPAIPLLIEGAKMTLWISVLGLAGGLVIGLLAGFAR
                     TFGGWIANHVALVFIEVIRGTPIVVQVMFIYFALPMAFNDLRIDPFTAAVVTIMINSG
                     AYIAEITRGAVLSIHKGFREAGLALGLSRWETIRYVILPLALRRMLPPLGNQWIISIK
                     DTSLFIVIGVAELTRQGQEIIAGNFRALEIWSAVAVFYLIITLVLSFILRRLERRMKI
                     L"
     gene            2553..3275
                     /gene="glnQ"
                     /locus_tag="NBI74_001190"
     CDS             2553..3275
                     /gene="glnQ"
                     /locus_tag="NBI74_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter ATP-binding protein
                     GlnQ"
                     /protein_id="HCD1931555.1"
                     /translation="MIEFKNVSKHFGPTQVLHNIDLNIAQGEVVVIIGPSGSGKSTLL
                     RCINKLEEITSGDLIVDGLKVNDPKVDERLIRQEAGMVFQQFYLFPHLTALENVMFGP
                     LRVRGANKEEAEKLARELLAKVGLAERAQHYPSELSGGQQQRVAIARALAVKPKMMLF
                     DEPTSALDPELRHEVLKVMQDLAEEGMTMVIVTHEIGFAEKVASRLIFIDKGRIAEDG
                     DPQVLIKNPPSQRLQEFLQHVS"
     gene            3392..5617
                     /gene="ybiO"
                     /locus_tag="NBI74_001191"
     CDS             3392..5617
                     /gene="ybiO"
                     /locus_tag="NBI74_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415329.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel protein"
                     /protein_id="HCD1931556.1"
                     /translation="MRWILFILFCLLGAPAHAVSIPGVTTTTTTDSTTEPAPEPDIEQ
                     KKAAYGALADVLDNDTSRKELIDQLRTVAATPPAEPVPKIVPPTLVEEQTVLQKVTEV
                     SRHYGEALSARFGQLYRNITGSPHKPFNPQTFSNALTHFSMLAVLVFGFYWLIRLCAL
                     PLYCKMGQWARQKNRERSNWLQLPAMIIGAFIIDLLLLALTLFVGQVLSDNLNAGSRT
                     IAFQQSLFLNAFALIEFFKTVLRLIFCPNVAELRPFTIHDETARYWSRRLSWLSSLIG
                     YGLIVAVPIISNQVNVQIGALANVIIMLCMTVWALYLIFRNKKEITQHLLNFAEHSLA
                     FFSLFIRAFALVWHWLASAYFIVLFFFSLFDPGNSLKFMMGATVRSLAIIGIAAFVSG
                     MFSRWLAKTITLSPHTQRNYPELQKRLNGWLSAALKTARILTVCVAVMLLLSAWGLFD
                     FWNWLQNGAGQKTVDILIRIALILFFSAVGWTVLASLIENRLASDIHGRPLPSARTRT
                     LLTLFRNALAVIISTITIMIVLSEIGVNIAPLLAGAGALGLAISFGSQTLVKDIITGV
                     FIQFENGMNTGDLVTIGPLTGTVERMSIRSVGVRQDTGAYHIIPWSSITTFANFVRGI
                     GSVVANYDVDRHEDADKANQALKDAVAELMENEEIRGLVIGEPNFAGIVGLSNTAFTL
                     RVSFTTLPLKQWTVRFALDSQVKKHFDLAGVRAPVQTYQVLPAPGAAPAEPLPPGEPT
                     L"
     gene            complement(5614..6540)
                     /gene="rlmF"
                     /locus_tag="NBI74_001192"
     CDS             complement(5614..6540)
                     /gene="rlmF"
                     /locus_tag="NBI74_001192"
                     /EC_number="2.1.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415328.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(1618)-N(6))-methyltransferase
                     RlmF"
                     /protein_id="HCD1931557.1"
                     /translation="MSAQKPGLHPRNRHHSRYDLATLCQVNPELRQFLTLTPAGEQSV
                     DFANPLAVKALNKALLAHFYAVANWDIPDGFLCPPVPGRADYIHHLADLLAEASGTIP
                     ANASILDIGVGANCIYPLIGVHEYGWRFTGSETSSQALSSAQAIISANPGLNRAIRLR
                     RQKESGAIFNGIIHKNEQYDATLCNPPFHDSAAAARAGSERKRRNLGLNKDDALNFGG
                     QQQELWCEGGEVAFIKKMIEESKGFAKQVMWFTSLVSRGENLPPLYRALTDVGAVKVV
                     KKEMAQGQKQSRFIAWTFMNDEQRRRFVNRQR"
     gene            6816..7076
                     /locus_tag="NBI74_001193"
     CDS             6816..7076
                     /locus_tag="NBI74_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HCD1931558.1"
                     /translation="MKKCLTLLIATVLSGISLTAYAAQPMSNLDSGQLRPAGTVSATG
                     ASNLSDLEDKLAEKAREQGAKGYVINSAGGNDQMFGTAIIYK"
     gene            7341..9623
                     /locus_tag="NBI74_001194"
     CDS             7341..9623
                     /locus_tag="NBI74_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore receptor Fiu"
                     /protein_id="HCD1931559.1"
                     /translation="MENNRNFPARQFHSLTFFAGLCIGITPVAQALAAEGQANADDTL
                     VVEASTPSLYAPQKSADPKFSRPVADTTRTMTVISEQVIKDQGATNLTDALKNVPGVG
                     AFFAGENGNSTTGDAIYMRGADTSNSIYIDGIRDIGSVSRDTFNTEQVEVIKGPSGTD
                     YGRSAPTGSINMISKQPRNDSGIDASASIGSAWFRRGTLDVNQVIGDTTAVRLNVMGE
                     KTHDAGRDKVKNERYGVAPSVAFGLGTANRLYLNYLHVTQHNTPDGGIPTIGLPGFSA
                     PSAGTAALNHSGKVDTHNFYGTDSDYDDSTTDTATMRFEHDINDNTTIRNTTRWSRVK
                     QDYLMTAIMGGASNITQPTSDVNSWTWSRTANTKDVSNKILTNQTNLTSTFYTGAIGH
                     DVSTGVEFTRETQTNYGVNPVTLPTVNIYHPDSSIHPGGLTRNGANANGQTDTFAIYA
                     FDTLQITRDFELNGGIRLDNYHTEYDSATACGGSGRGAITCPAGVAKGSPVTTVDTAK
                     SGNLVNWKAGALYHLTENGNVYINYAVSQQPPGGNNFALAQSGSGNSANRTDFKPQKA
                     NTSEIGTKWQVLDKRLLLTAALFRTDIENEVEQNDDGTYSQYGKKRVEGYEISVAGNI
                     TPAWQMIGGYTQQKATIKNGKDVAQDGSSSLPYTPEHAFTLWSQYQATDDISVGAGAR
                     YIGSMHKGSDGAVGTPAFTEGYWVADAKLGYRVNRNLDFQLNVYNLFDTDYVASINKS
                     GYRYHPGEPRTFLLTANMHF"
     gene            9665..10342
                     /gene="ybiX"
                     /locus_tag="NBI74_001195"
     CDS             9665..10342
                     /gene="ybiX"
                     /locus_tag="NBI74_001195"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKHD-type hydroxylase YbiX"
                     /protein_id="HCD1931560.1"
                     /translation="MMYHIPGVLSPQDVARFREHLEQAEWVDGRVTTGAQGAQVKNNQ
                     QVDTRSALYAALQNEVLNAVNQHALFFAAALPRTLSTPLFNRYQNNETYGFHVDGAVR
                     SHPQNGWMRTDLSATLFLSDPESYDGGELVVNDTFGQHRVKLPAGDLVLYPSSSLHCV
                     TPVTRGVRVASFMWIQSMIRDDKKRAMLFELDNNIQSLKSHYGESEEILSLLNLYHNL
                     LREWSEI"
     gene            10416..10682
                     /gene="ybiI"
                     /locus_tag="NBI74_001196"
     CDS             10416..10682
                     /gene="ybiI"
                     /locus_tag="NBI74_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc finger protein YbiI"
                     /protein_id="HCD1931561.1"
                     /translation="MASGWANDDAVNEQINSTIEDAIARARGEIPRGESLYECEECGA
                     PIPQARREAIPGVRLCIHCQQEKDLQKPAYTGYNRRGSKDSQLR"
     gene            10947..11207
                     /locus_tag="NBI74_001197"
     CDS             10947..11207
                     /locus_tag="NBI74_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HCD1931562.1"
                     /translation="MKTINTVVAAMALSTLSFGVFAAEPVTASQAQNMNKIGVVSADG
                     ASTLDALEAKLAEKAAAAGASGYSITSATNNNKLSGTAVIYK"
     gene            complement(11345..12430)
                     /gene="hcxB"
                     /locus_tag="NBI74_001198"
     CDS             complement(11345..12430)
                     /gene="hcxB"
                     /locus_tag="NBI74_001198"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcXB"
                     /protein_id="HCD1931563.1"
                     /translation="MESGHRFDAQTLHSFIQAVFRQMGSEEQEAKLVADHLIAANLAG
                     HDSHGIGMIPSYVRSWSQGHLQINHHAKVVKEAGAAVTLDGDRAFGQVAAHEAMALGI
                     EKAHQHGIAAVALHNSHHIGRIGYWAEQCAAAGFVSIHFVSVVGIPMVAPFHGRDSRF
                     GTNPFCVVFPRKDNFPLLLDYATSAIAFGKTRVAWHKGVPVPPGCLIDVNGVPTTNPA
                     VMQESPLGSLLTFAEHKGYALAAMCEILGGALSGGKTTHQETLQTSPDAILNCMTTII
                     INPELFGAPDCSAQTEAFAEWVKASPHDDDKPILLPGEWEVNTRRERQEQGIPLDAGS
                     WQAICDAARQIGMPEETLQAFCQQLAS"
     gene            complement(12570..13532)
                     /gene="ybiB"
                     /locus_tag="NBI74_001199"
     CDS             complement(12570..13532)
                     /gene="ybiB"
                     /locus_tag="NBI74_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein YbiB"
                     /protein_id="HCD1931564.1"
                     /translation="MDYRKIIKEIGRGKNHARDLDRDTARGLYAHMLNGEVPDLELGG
                     VLIALRIKGEGEAEMLGFYEAMQNHTIKLTPPAGKPMPIVIPSYNGARKQANLTPLLA
                     ILLHKLGFPVVVHGVSEDPTRVLTETIFELMGITPTLHGGQAQAKLDEHQPVFMPVGA
                     FCPPLEKQLAMRWRMGVRNSAHTLAKLATPFAEGEALRLSSVSHPEYIGRVAKFFSDI
                     GGRALLMHGTEGEVYANPQRCPQINLIDREGMRVLYEKQDTAGSELLPQAKDPETTAQ
                     WIERCLAGSEPIPESLKIQMACCLMATGEAATISDGLARVNQAF"
     gene            complement(13560..15710)
                     /gene="dinG"
                     /locus_tag="NBI74_001200"
     CDS             complement(13560..15710)
                     /gene="dinG"
                     /locus_tag="NBI74_001200"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase DinG"
                     /protein_id="HCD1931565.1"
                     /translation="MALTAALKAQIAAWYKALQEQIPDFIPRAPQRQMIADVAKTLAG
                     EEGRHLAIEAPTGVGKTLSYLIPGIAIAREEQKTLVVSTANVALQDQIYSKDLPLLKK
                     IIPDLKFTAAFGRGRYVCPRNLTALASTEPTQQDLLAFLDDELTPNNQEEQKRCAKLK
                     GDLDTYKWDGLRDHTDIAIDDDLWRRLSTDKASCLNRNCYYYRECPFFVARREIQEAE
                     VVVANHALVMAAMESEAVLPDPKNLLLVLDEGHHLPDVARDALEMSAEITAPWYRLQL
                     DLFTKLVATCMEQFRPKTIPPLAIPERLNAHCEELYELIASLNNILNLYMPAGQEAEH
                     RFAMGELPDEVLEICQRLAKLTEMLRGLAELFLNDLSEKTGSHDIVRLHRLILQMNRA
                     LGMFEAQSKLWRLASLAQSSGAPVTKWATREEREGQLHLWFHCVGIRVSDQLERLLWR
                     SIPHIIVTSATLRSLNSFSRLQEMSGLKEKAGDRFVALDSPFNHCEQGKIVIPRMRVE
                     PSIDNEEQHIAEMAAFFREQVESKKHLGMLVLFASGRAMQRFLDYVTDLRLMLLVQGD
                     QPRYRLVELHRKRVANGERSVLVGLQSFAEGLDLKGALLSQVHIHKIAFPPIDSPVVI
                     TEGEWLKSLNRYPFEVQSLPSASFNLIQQVGRLIRSHGCWGEVVIYDKRLLTKNYGKR
                     LLDALPVFPIEQPEVPEGIVKKKEKTKSPRRRRR"
     gene            15814..16014
                     /locus_tag="NBI74_001201"
                     /pseudo
     CDS             15814..16014
                     /locus_tag="NBI74_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706676.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1768 domain-containing protein"
     gene            complement(16247..17608)
                     /gene="rhlE"
                     /locus_tag="NBI74_001202"
     CDS             complement(16247..17608)
                     /gene="rhlE"
                     /locus_tag="NBI74_001202"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlE"
                     /protein_id="HCD1931566.1"
                     /translation="MSFDSLGLSPDILRAVAEQGYREPTPIQQQAIPAVLEGRDLMAS
                     AQTGTGKTAGFTLPLLQHLITRQPHAKGRRPVRALILTPTRELAAQIGENVRDYSKYL
                     NIRSLVVFGGVSINPQMMKLRGGVDVLVATPGRLLDLEHQNAVKLDQVEILVLDEADR
                     MLDMGFIHDIRRVLTKLPAKRQNLLFSATFSDDIKALAEKLLHNPLEIEVARRNTASD
                     QVTQHVHFVDKKRKRELLSHMIGKGNWQQVLVFTRTKHGANHLAEQLNKDGIRSAAIH
                     GNKSQGARTRALADFKSGDIRVLVATDIAARGLDIEELPHVVNYELPNVPEDYVHRIG
                     RTGRAAATGEALSLVCVDEHKLLRDIEKLLKKEIPRIAILGYEPDPSIKAEPIQNGRQ
                     QRGGGRGQGGGRGQQQPRRAESGAKSGNAKPAEKPSRRLGDAKPAGEQQRRRRPRKPA
                     AAQ"
     gene            17837..18508
                     /gene="cecR"
                     /locus_tag="NBI74_001203"
     CDS             17837..18508
                     /gene="cecR"
                     /locus_tag="NBI74_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CecR"
                     /protein_id="HCD1931567.1"
                     /translation="MNNPAMTIKGEQAKKQLIAAALAQFGEYGMNATTREIAAQAGQN
                     IAAITYYFGSKEDLYLACAQWIADFIGEQFRPHAEEAERLFAQPQPDRAAIRELILRA
                     CRNMIKLLTQDDTVNLSKFISREQLSPTAAYHLVHEQVISPLHSHLTRLIAAWTGCDA
                     SDTRMILHTHALIGEILAFRLGKETILLRTGWTAFDEEKTELINQTVTCHIDLILQGL
                     SQRSL"
     gene            18511..19506
                     /gene="hlyD"
                     /locus_tag="NBI74_001204"
     CDS             18511..19506
                     /gene="hlyD"
                     /locus_tag="NBI74_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein HlyD"
                     /protein_id="HCD1931568.1"
                     /translation="MKKPVVIGLAVVVLAAVVAGGYWWYQSRQDNGLTLYGNVDIRTV
                     NLSFRVGGRVESLAVDEGDAIKAGQVLGELDHKPYEIALMQAKAGVSVAQAQYDLMLA
                     GYRDEEIAQAAAAVKQAQAAYDYAQNFYNRQQGLWKSRTISANDLENARSSRDQAQAT
                     LKSAQDKLRQYRSGNREQDIAQAKASLEQAQAQLAQAELNLQDSTLIAPSDGTLLTRA
                     VEPGTVLNEGGTVFTVSLTRPVWVRAYVDERNLDQAQPGRKVLLYTDGRPDKPYHGQI
                     GFVSPTAEFTPKTVETPDLRTDLVYRLRIVVTDADDALRQGMPVTVQFGDEAGHE"
     gene            19499..21235
                     /locus_tag="NBI74_001205"
     CDS             19499..21235
                     /locus_tag="NBI74_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415315.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCD1931569.1"
                     /translation="MNDAVITLNGLEKRFPGMDKPAVAPLDCTIHAGYVTGLVGPDGA
                     GKTTLMRMLAGLLKPDSGSATVIGFDPIKNDGALHAVLGYMPQKFGLYEDLTVMENLN
                     LYADLRSVTGEARKQTFARLLEFTSLGPFTGRLAGKLSGGMKQKLGLACTLVGEPKVL
                     LLDEPGVGVDPISRRELWQMVHELAGEGMLILWSTSYLDEAEQCRDVLLMNEGELLYQ
                     GEPTALTQTMAGRSFLMTSPHEGNRKLLQRALKLPQVSDGMIQGKSVRLILKKETTPD
                     DIRHADGMPEININETTPRFEDAFIDLLGGAGTSESPLGAILHTVEGTPGETVIEAKE
                     LTKKFGDFAATDHVNFAVKRGEIFGLLGPNGAGKSTTFKMMCGLLVPTSGQALVLGMD
                     LKESSGKARQHLGYMAQKFSLYGNLTVEQNLRFFSGVYGLRGRAQNEKISRMSEAFGL
                     KSIASHATDELPLGFKQRLALACSLMHEPDILFLDEPTSGVDPLTRREFWLHINSMVE
                     KGVTVMVTTHFMDEAEYCDRIGLVYRGKLIASGTPDDLKAQSANDEQPDPTMEQAFIQ
                     LIHDWDKEHSNE"
     gene            21228..22361
                     /locus_tag="NBI74_001206"
     CDS             21228..22361
                     /locus_tag="NBI74_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCD1931570.1"
                     /translation="MSNPILSWRRVRALCVKETRQIVRDPSSWLIAVVIPLLLLFIFG
                     YGINLDSSKLRVGILLEQRSEAALDFTHTMTGSPYIDATISDNRQELIAKMQAGKIRG
                     LVVIPVDFAEQMERANAIAPIQVITDGSEPNTANFVQGYVEGIWQIWQMQRAEDNGQT
                     FEPLIDVQTRYWFNPAAISQHFIIPGAVTIIMTVIGAILTSLVVAREWERGTMEALLS
                     TEITRTELLLCKLIPYYFLGMLAMLLCMLVSVFILGVPYRGSLLILFFISSLFLLSTL
                     GMGLLISTITRNQFNAAQVALNAAFLPSIMLSGFIFQIDSMPAVIRAVTYIIPARYFV
                     STLQSLFLAGNIPVVLIVNVLFLIASAVMFIGLTWLKTKRRLD"
     gene            22372..23478
                     /locus_tag="NBI74_001207"
     CDS             22372..23478
                     /locus_tag="NBI74_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCD1931571.1"
                     /translation="MFHRLWTLIRKELQSLLREPQTRAILILPVLIQVILFPFAATLE
                     VTNATIAIYDEDNGEHSVELTQRFARASAFTHVLLLKSPQEIRPTIDTQKALLLVRFP
                     ADFSRKLDTFQTAPLQLILDGRNSNSAQIAANYLQQIVKNYQQELLEGKPKPNNSELV
                     VRNWYNPNLDYKWFVVPSLIAMITTIGVMIVTSLSVAREREQGTLDQLLVSPLTTWQI
                     FIGKAVPALIVATFQATIVLAIGIWAYQIPFAGSLALFYFTMVIYGLSLVGFGLLISS
                     LCSTQQQAFIGVFVFMMPAILLSGYVSPVENMPVWLQNLTWINPIRHFTDITKQIYLK
                     DASLDIVWNSLWPLLVITATTGSAAYAMFRRKVM"
     gene            complement(23440..23850)
                     /locus_tag="NBI74_001208"
     CDS             complement(23440..23850)
                     /locus_tag="NBI74_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhQ family protein"
                     /protein_id="HCD1931572.1"
                     /translation="MKWQQRVRVATGLSCWQIMLHLLVVALLVVGWMSKTLVHVGVGL
                     CALYCVTVVMMLVFQRHPEQRWREVADVLEELTTTWYFGAALIVLWLLSRVLENNFLL
                     AIAGLAILAGPAVVSLLAKDKKLHHLTSKHRVRR"
     gene            23983..24744
                     /locus_tag="NBI74_001209"
     CDS             23983..24744
                     /locus_tag="NBI74_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HCD1931573.1"
                     /translation="MPDQTQQFSFKVLTINIHKGFTAFNRRFILPELRDAVRTVSADI
                     VCLQEVMGAHEVHPLHVENWPDTSHYEFLADTMWSDFAYGRNAVYPEGHHGNAVLSRY
                     PIEHYENRDVSVDGAEKRGVLYCRIVPPMTGKAIHVMCVHLGLREAHRQAQLAMLAEW
                     VNELPDGESVLVAGDFNDWRQKANHPLKVLAGLDEIFTRAHGRPARTFPVQFPLLRLD
                     RIYVKNASASAPTALPLRTWRHLSDHAPLSAEIHL"
     gene            24741..25982
                     /gene="clsB"
                     /locus_tag="NBI74_001210"
     CDS             24741..25982
                     /gene="clsB"
                     /locus_tag="NBI74_001210"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsB"
                     /protein_id="HCD1931574.1"
                     /translation="MKCSWREGNMIQLLENGEQYYPAVFKAIGEAQERIILETFIWFE
                     DDVGKQLHAALLAAAQRGVKAEVLLDGYGSPDLSDEFVNELTAAGVVFRYYDPRPRLF
                     GMRTNVFRRMHRKIVVIDARIAFIGGLNYSAEHMSSYGPEAKQDYAVRLEGPIVEDIL
                     QFELENLPGQSTARRWWRRHHKAEENRQPGEAQVLLVWRDNEEHRDDIERHYLKMLTQ
                     ARREVIIANAYFFPGYRFLHALRKAARRGVRIKLIIQGEPDMPIVRVGARLLYNYLVK
                     GGVQVFEYRRRPLHGKVALMDDHWATVGSSNLDPLSLSLNLEANVIIHDRNFNQTLRD
                     NLNGIIAADCQQVDETMLPKRTWWNLTKSVLAFHFLRHFPALVGWLPAHTPRLAQVDP
                     PAQPTMETQDRVETENTGVKP"
     gene            25982..26938
                     /locus_tag="NBI74_001211"
     CDS             25982..26938
                     /locus_tag="NBI74_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0104 family protein"
                     /protein_id="HCD1931575.1"
                     /translation="MSKSHPRWRLAKKILTWLFFIAVIVLLVVYAKKVDWEEVWKVIR
                     DYNRVALLSAVGLVVVSYLIYGCYDLLARFYCGHKLAKRQVMLVSFICYAFNLTLSTW
                     VGGIGMRYRLYSRLGLPGSTITRIFSLSITTNWLGYILLAGVIFTAGVVELPDHWYVD
                     QTTLRILGIGLLMIIAVYLWFCAFAKHRHMTIKGQKLVLPSWKFALAQMLISSVNWMV
                     MGAIIWLLLGQSVSYFFVLGVLLVSSIAGVIVHIPAGIGVLEAVFIALLAGEHTSKGT
                     IIAALLAYRVLYYFIPLLLALICYLLLESQAKKLRAKNEAAM"
     gene            complement(26974..27687)
                     /locus_tag="NBI74_001212"
     CDS             complement(26974..27687)
                     /locus_tag="NBI74_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HCD1931576.1"
                     /translation="MESYSQNSNKLDFQHEARILNGIWLITALGLVATAGLAWGAKYL
                     EITATKYDSPQMYVAIGLLLLSMYGLSKDINKINTAIAGVIYLFLISLVAIVVASLAP
                     VSAIIVVFSTAGSMFLISMLAGLLFKFDPGSHRFIIMMTLTGLALVIIVNATLMSERP
                     IWVISCLMIVLWSGIISHGRNKLLELTGKSHSEELWSPVRCAFIGALTLYYYFIGFFG
                     ILAAIAITLVWQRHTRFFH"
     gene            complement(27893..28597)
                     /locus_tag="NBI74_001213"
     CDS             complement(27893..28597)
                     /locus_tag="NBI74_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HCD1931577.1"
                     /translation="MDRFPRSDSIVQPRAGLQTYMAQVYGWMTVGLLLTAFVAWYAAN
                     SAAVMELLFTNRVFLIGLIIAQLALVIVLSAMIQKLSAGVTTMLFMLYSALTGLTLSS
                     IFIVYTAASIASTFVVTAGMFGAMSLYGYTTKRDLSGFGNMLFMALIGIVLASLVNFW
                     LKSEALMWAVTYIGVIVFVGLTAYDTQKLKNMGEQIDTRDTSNLRKYSILGALTLYLD
                     FINLFLMLLRIFGNRR"
     gene            complement(28734..29186)
                     /gene="moaE"
                     /locus_tag="NBI74_001214"
     CDS             complement(28734..29186)
                     /gene="moaE"
                     /locus_tag="NBI74_001214"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase catalytic subunit MoaE"
                     /protein_id="HCD1931578.1"
                     /translation="MAETKIVVGPQPFSVGEEYPWLAERDEDGAVVTFTGKVRNHNLG
                     DSVKALTLEHYPGMTEKALAEIVDEARNRWPLGRVTVIHRIGELWPGDEIVFVGVTSA
                     HRSSAFEAGQFIMDYLKTRAPFWKREATPEGDRWVEARESDQQAAKRW"
     gene            complement(29188..29433)
                     /gene="moaD"
                     /locus_tag="NBI74_001215"
     CDS             complement(29188..29433)
                     /gene="moaD"
                     /locus_tag="NBI74_001215"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase sulfur carrier subunit"
                     /protein_id="HCD1931579.1"
                     /translation="MINVLFFAQVRELVGTDATEVAADFPTVEALRQHLAAQSDRWAL
                     ALEDGKLLAAVNQTLVSFDHSLTDGDEVAFFPPVTGG"
     gene            complement(29426..29911)
                     /gene="moaC"
                     /locus_tag="NBI74_001216"
     CDS             complement(29426..29911)
                     /gene="moaC"
                     /locus_tag="NBI74_001216"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HCD1931580.1"
                     /translation="MSQLTHINAAGEAHMVDVSAKAETVREARAEAFVTMRSETLAMI
                     IDGRHHKGDVFATARIAGIQAAKRTWDLIPLCHPLMLSKVEVNLQAEPEHNRVRIETL
                     CRLTGKTGVEMEALTAASVAALTIYDMCKAVQKDMVIGPVRLLAKSGGKSGDFKVEAD
                     D"
     gene            complement(29914..30426)
                     /gene="moaB"
                     /locus_tag="NBI74_001217"
     CDS             complement(29914..30426)
                     /gene="moaB"
                     /locus_tag="NBI74_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein B"
                     /protein_id="HCD1931581.1"
                     /translation="MSQVSTEFIPTRIAILTVSNRRGEEDDTSGHYLRDSAQEAGHHV
                     VDKAIVKENRYAIRAQVSAWIASDDVQVVLITGGTGLTEGDQAPEALLPLFDREVEGF
                     GEVFRMLSFEEIGTSTLQSRAVAGVANKTLIFAMPGSTKACRTAWENIIAPQLDARTR
                     PCNFHPHLKK"
     gene            complement(30448..31437)
                     /gene="moaA"
                     /locus_tag="NBI74_001218"
     CDS             complement(30448..31437)
                     /gene="moaA"
                     /locus_tag="NBI74_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HCD1931582.1"
                     /translation="MASQLTDAFARKFYYLRLSITDVCNFRCTYCLPDGYKPSGVTNK
                     GFLTVDEIRRVTRAFASLGTEKVRLTGGEPSLRRDFTDIIAAVRENDAIRQIAVTTNG
                     YRLERDVANWRDAGLTGINVSVDSLDARQFHAITGQDKFNQVMAGIDAAFEAGFEKVK
                     VNTVLMRDVNHHQLDTFLNWIQHRPIQLRFIELMETGEGSELFRKHHISGQVLRDELL
                     RRGWIHQLRQRSDGPAQVFCHPDYAGEIGLIMPYEKDFCATCNRLRVSSIGKLHLCLF
                     GEGGVNLRDLLENDTQQQALEARISAALREKKQTHFLHQNNTGITQNLSYIGG"
     regulatory      complement(31425..31569)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01055"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="molybdenum cofactor riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="molybdenum cofactor"
                     /db_xref="RFAM:RF01055"
     gene            31834..32742
                     /gene="yvcK"
                     /locus_tag="NBI74_001219"
     CDS             31834..32742
                     /gene="yvcK"
                     /locus_tag="NBI74_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HCD1931583.1"
                     /translation="MRNRTLADLDRVVALGGGHGLGRVLSSLSSLGSRLTGIVTTTDN
                     GGSTGRIRRSEGGIAWGDMRNCLNQLITEPSVASAMFEYRFGGNGELSGHNLGNLMLK
                     ALDHLSVRPLEAINLIRNLLKVDAHLIPMSEHPVDLMAIDDQGHEVYGEVNIDQLTAP
                     IQELLLTPNVPATREAVHAISEADLIIIGPGSFYTSLMPILLLKEIAQALRRTPAPMV
                     YIGNLGRELSLPAANLKLESKLAIMEQYVGKKVIDAVIVGPKVDVSAVKERIVIQEVL
                     EASDIPYRHDRQLLHSALEKALQALG"
     gene            complement(32780..34801)
                     /gene="uvrB"
                     /locus_tag="NBI74_001220"
     CDS             complement(32780..34801)
                     /gene="uvrB"
                     /locus_tag="NBI74_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit B"
                     /protein_id="HCD1931584.1"
                     /translation="MSKPFKLNSAFKPSGDQPEAIRRLEEGLEDGLAHQTLLGVTGSG
                     KTFTIANVIADLQRPTMVLAPNKTLAAQLYGEMKEFFPENAVEYFVSYYDYYQPEAYV
                     PSSDTFIEKDASVNEHIEQMRLSATKAMLERRDVVVVASVSAIYGLGDPDLYLKMMLH
                     LTVGMIIDQRAILRRLAELQYARNDQAFQRGTFRVRGEVIDIFPAESDDIALRVELFD
                     EEVERLSLFDPLTGQIVSTIPRFTIYPKTHYVTPRERIVQAMEEIKEELAARRKVLLE
                     NNKLLEEQRLTQRTQFDLEMMNELGYCSGIENYSRFLSGRGPGEPPPTLFDYLPADGL
                     LVVDESHVTIPQIGGMYRGDRARKETLVEYGFRLPSALDNRPLKFEEFEALAPQTIYV
                     SATPGNYELEKSGGDVVDQVVRPTGLLDPIIEVRPVATQVDDLLSEIRQRAAINERVL
                     VTTLTKRMAEDLTEYLEEHGERVRYLHSDIDTVERMEIIRDLRLGEFDVLVGINLLRE
                     GLDMPEVSLVAILDADKEGFLRSERSLIQTIGRAARNVNGKAILYGDKITPSMAKAIG
                     ETERRREKQQKYNEEHGITPQGLNKKVVDILALGQNIAKTKAKGRGKSRPIVEPDNVP
                     MDMSPKALQQKIHELEGLMMQHAQNLEFEEAAQIRDQLHQLRELFIAAS"
     gene            complement(35380..36057)
                     /gene="bioD"
                     /locus_tag="NBI74_001221"
     CDS             complement(35380..36057)
                     /gene="bioD"
                     /locus_tag="NBI74_001221"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent dethiobiotin synthetase BioD"
                     /protein_id="HCD1931585.1"
                     /translation="MSKCYFVTGTDTEVGKTVASCALLQAAKAAGYRTAGYKPVASGS
                     EKTPKGLRNSDALALQRNSSLQLDYATVNPYTFAEPTSPHIISAQEGRPIEALVMSAG
                     LRALEQQADWVLVEGAGGWFTPLSDTFTFADWVTQEQLPVILVVGVKLGCINHAMLTA
                     QAILHAGLTLAGWVANDVTPPGKRHAEYMTTLTRMIPAPLLGEIPWLAENPENAATGK
                     YINLALM"
     gene            complement(36050..36805)
                     /gene="bioC"
                     /locus_tag="NBI74_001222"
     CDS             complement(36050..36805)
                     /gene="bioC"
                     /locus_tag="NBI74_001222"
                     /EC_number="2.1.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-ACP O-methyltransferase BioC"
                     /protein_id="HCD1931586.1"
                     /translation="MATVNKQAIAAAFGRAAAHYEQHADLQRQSADALLAMLPQRKYT
                     RVLDAGCGPGWMSRRWRERHAQVTALDLSPPMLVQARQKDAADHYLAGDIESLPLATA
                     TFDLAWSNLAVQWCGNLSTALRELYRVVRPGGVVAFTTLVQGSLPELHQAWQAVDERP
                     HANRFLPPDEIEQSLNAVHYQHYIQPITLWFDDALSAMRSLKGIGATHLHEGRDPRIL
                     TRSQLQRLQLAWPQQQGRYPLTYHLFLGVIARE"
     gene            complement(36792..37946)
                     /gene="bioF"
                     /locus_tag="NBI74_001223"
     CDS             complement(36792..37946)
                     /gene="bioF"
                     /locus_tag="NBI74_001223"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HCD1931587.1"
                     /translation="MSWQEKINAALDARRAADALRRRYPVAQGAGRWLVVDDRQYLNF
                     SSNDYLGLSHHPQIIRAWKQGAEQFGIGSGGSGHVSGYSVAHQALEEELAEWLGYSRA
                     LLFISGFAANQAVIAAMMAKEDRIVADRLSHASLLEAASLSPSPLRRFAHNDVTHLAR
                     LLASPCPGQQLVVTEGVFSMDGDSAPLEEIQQVTQQHDGWLMVDDAHGTGVIGEQGRG
                     SCWLQKVKPELLVVTFGKGFGVSGAAVLCSNTVADYLLQFARHLIYSTSMPPAQAQAL
                     RASLAVIRSDEGDARREKLAALITRFRAGVQDLPFTLADSWSAIQPLIVGDNSRALQL
                     AEKLRQQGCWVTAIRPPTVPAGTARLRLTLTAAHEMQDIDRLLEVLHGNG"
     gene            complement(37943..38983)
                     /gene="bioB"
                     /locus_tag="NBI74_001224"
     CDS             complement(37943..38983)
                     /gene="bioB"
                     /locus_tag="NBI74_001224"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HCD1931588.1"
                     /translation="MAHRPRWTLSQVTELFEKPLLDLLFEAQQVHRQHFDPRQVQVST
                     LLSIKTGACPEDCKYCPQSSRYKTGLEAERLMEVEQVLESARKAKAAGSTRFCMGAAW
                     KNPHERDMPYLEQMVQGVKAMGLEACMTLGTLSESQAQRLANAGLDYYNHNLDTSPEF
                     YGNIITTRTYQERLDTLEKVRDAGIKVCSGGIVGLGETVKDRAGLLLQLANLPTPPES
                     VPINMLVKVKGTPLADNDDVDAFDFIRTIAVARIMMPTSYVRLSAGREQMNEQTQAMC
                     FMAGANSIFYGCKLLTTPNPEEDKDLQLFRKLGLNPQQTAVLAGDNEQQQRLEQALMT
                     PDTDEYYNAAAL"
     gene            39070..40359
                     /gene="bioA"
                     /locus_tag="NBI74_001225"
     CDS             39070..40359
                     /gene="bioA"
                     /locus_tag="NBI74_001225"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HCD1931589.1"
                     /translation="MTTDDLAFDQRHIWHPYTSMTSPLPVYPVASAEGCELILSDGRR
                     LVDGMSSWWAAIHGYNHPQLNAAMKSQIDAMSHVMFGGITHAPAIELCRKLVAMTPQP
                     LECVFLADSGSVAVEVAMKMALQYWQAKGEARQRFLTFRNGYHGDTFGAMSVCDPDNS
                     MHNLWKGYLPENLFAPAPQSRMDGEWDERDMVGFARLMAAHRHEIAAVIIEPIVQGAG
                     GMRMYHPEWLKRIRKMCDREGILLIADEIATGFGRTGKLFACEHAEIAPDILCLGKAL
                     TGGTMTLSATLTTREVAETISNGEAGCFMHGPTFMGNPLACAAANASLAILESGDWQH
                     QVAAIEAQLREQLAPACDAEMVADVRVLGAIGVVETSRPVNMAALQKFFVEQGVWIRP
                     FGKLIYLMPPYIILPQQLQRLTAAVNRAVQDETFFCQ"
     gene            40418..40894
                     /locus_tag="NBI74_001226"
     CDS             40418..40894
                     /locus_tag="NBI74_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase inhibitor"
                     /protein_id="HCD1931590.1"
                     /translation="MKLISNDLRDGDKLPHRHVFNGMGYDGDNISPHLAWDDVPVGTK
                     SFVVTCYDPDAPTGSGWWHWVVVNLPADTRVLPQGFGSGLVAMPDGVLQTRTDFGKTG
                     YDGAAPPKGETHRYIFTVHALDVERIDVDEGASGAMVGFNVHFHSLASASITAMFS"
     gene            41046..42329
                     /locus_tag="NBI74_001227"
     CDS             41046..42329
                     /locus_tag="NBI74_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative acyl-CoA thioester hydrolase"
                     /protein_id="HCD1931591.1"
                     /translation="MNTFSVSRLALALAFGVTLTACSSTPPDQRPSDQTAPGTSSRPI
                     LSAKEAQNFDAQHYFASLTPGAAAWNPSPITLPAQPDFVVGPAGTQGVTHTTIQAAVD
                     AAIIKRTNKRQYIAVMPGEYQGTVYVPAAPGGITLYGTGEKPIDVKIGLSLDGGMSPA
                     DWRHDVNPRGKYMPGKPAWYMYDSCQSKRSDSIGVLCSAVFWSQNNGLQLQNLTIENT
                     LGDSVDAGNHPAVALRTDGDKVQINNVNILGRQNTFFVTNSGVQNRLETNRQPRTLVT
                     NSYIEGDVDIVSGRGAVVFDNTEFRVVNSRTQQEAYVFAPATLSNIYYGFLAVNSRFN
                     ASGDGVAQLGRSLDVDANTNGQVVIRDSAINEGFNTAKPWADAVISNRPFAGNTGSVD
                     DNDEVQRNLNDTNYNRMWEYNNRGVGSKVVAEAKK"
     gene            complement(42470..44731)
                     /locus_tag="NBI74_001228"
     CDS             complement(42470..44731)
                     /locus_tag="NBI74_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415292.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydratase"
                     /protein_id="HCD1931592.1"
                     /translation="MIKLSEKGVFLASKNEIIAEEHFTGEIKKEEAKKGTIAWSILSS
                     HNTSGNMDKLKIKFDSLASHDITFVGIVQTAKASGMERFPLPYVLTNCHNSLCAVGGT
                     INGDDHVFGLSAAQRYGGIFVPPHIAVIHQYMREMMAGGGKMILGSDSHTRYGALGTM
                     AVGEGGGELVKQLLNDTWDIDYPGVVAVHLTGKPAPYVGPQDVALAIIGAVFKNGYVK
                     NKVMEFVGPGVAALSTDFRNSVDVMTTETTCLSSVWQTDEEVHNWLALHGRGQDYCQL
                     NPQPMAYYDGCISVDLSAIKPMIALPFHPSNVYEIDTLNQNLTDILREIEIESERVAH
                     GKAKLSLLDKVENGRLKVQQGIIAGCSGGNYENVIAAANALRGQSCGNDTFSLAVYPS
                     SQPVFMDLAKKGVVADLIGAGAIIRTAFCGPCFGAGDTPINNGLSIRHTTRNFPNREG
                     SKPANGQMSAVALMDARSIAATAANGGYLTSASELDCWDNVPEYAFDVTPYKNRVYQG
                     FVKGATQQPLIYGPNIKDWPELGALTDNIVLKVCSKILDEVTTTDELIPSGETSSYRS
                     NPIGLAEFTLSRRDPGYVGRSKATAELENQRLAGNVSEMAEVFARIKQIAGQEHVDPL
                     QTEIGSMVYAVKPGDGSAREQAASCQRVIGGLANIAEEYATKRYRSNVINWGMLPLQM
                     AEAPTFEVGDYIYIPGIKAALDNPGTTFKGYVIHEDAPVTEITLYMESLTAEEREIIK
                     AGSLINFNKNRQM"
     gene            complement(44914..46347)
                     /locus_tag="NBI74_001229"
     CDS             complement(44914..46347)
                     /locus_tag="NBI74_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion permease"
                     /protein_id="HCD1931593.1"
                     /translation="MNKKSLWKLILILAIPCIIGFMPAPAGLSELAWVLFGIYLAAIV
                     GLVIKPFPEPVVLLIAVAASMVVVGNLSGGEFKTTAVLSGYSSGTTWLVFSAFTLSAA
                     FVTTGLGKRIAYLLIGKIGSTTLGLGYVTVFLDLVLAPATPSNTARAGGIVLPIINSV
                     AVALGSEPEKSPRRVGHYLMMSIYMVTKTTSYMFFTAMAGNILALKMINDILHLQISW
                     GGWALAAGLPGIIMLLVTPLVIYTMYPPEIKKVDNKTIAKAGLAELGPMKIREKMLLG
                     VFVLALLGWIFSKSLGVDESTVAIVVMATMLLLGIVTWEDVVKNKGGWNTLIWYGGII
                     GLSSLLSKVKFFEWLAEVFKNNLAFDGHGNVAFFVIIFLSIIVRYFFASGSAYIVAML
                     PVFAMLANVSGAPLMLTALALLFSNSYGGMVTHYGGAAGPVIFGVGYNDIKSWWLVGA
                     VLTILTFLVHITLGVWWWNMLIGWNML"
     gene            complement(46423..47475)
                     /locus_tag="NBI74_001230"
     CDS             complement(46423..47475)
                     /locus_tag="NBI74_001230"
                     /EC_number="5.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalomesaconate tautomerase"
                     /protein_id="HCD1931594.1"
                     /translation="MKKIPCVMMRGGTSRGAFLLAEHLPEDQTQRDKILMAIMGSGND
                     LEIDGIGGGNPLTSKVAIISRSSDPRADVDYLFAQVIVHEQRVDTTPNCGNMLSGVGA
                     FAIENGLIAATSPVTRVRIRNVNTGTFIEADVQTPNGVVEYEGSARIDGVPGTAAPVA
                     LTFLNAAGTKTGKVFPTDNQIDYFDDVPVTCIDMAMPVVIIPAEYLGKTGYELPAELD
                     ADKALLARIESIRLQAGKAMGLGDVSNMVIPKPVLISPAQKGGAINVRYFMPHSCHRA
                     LAITGAIAISSSCALEGTVTRQIVPSVGYGNINIEHPSGALDVHLSNEGQDATTLRAS
                     VIRTTRKIFSGEVYLP"
BASE COUNT        11283 a        12698 c        12199 g        11326 t
ORIGIN      
        1 ccactattag tgtgatagga acagccagaa tagcggaaca catagctggt gctatactta
       61 gtctcgttaa ttactgggac ataacatcaa gaggatatga aattatgagt accgctaaat
      121 tagttaaatc aaaagcgacc aatctgcttt atacccgcaa cgatgtctct gacagcgaga
      181 aaaaagcaac agtagagttg ctgaatcgcc aggttatcca gtttattgat ctttctttga
      241 ttaccaaaca agcgcactgg aacatgcgcg gcgctaactt cattgccgta catgaaatgc
      301 tggatggctt ccgcactgca ctgatcgatc atctggatac catggcagaa cgtgcagtgc
      361 agctgggcgg tgtagctctg gggaccactc aagttatcaa cagcaaaact ccgctgaaaa
      421 gttacccgct ggacatccac aacgttcagg atcacctgaa agagctggct gaccgttacg
      481 caatcgtcgc taatgacgta cgcaaagcga ttggcgaagc gaaagatgac gacaccgcag
      541 atatcctgac cgccgcgtct cgcgacctgg ataaattcct gtggtttatc gagtctaaca
      601 tcgaataaat ccatcgctga tggtgcagaa ctttagtaca cgataaaagc ggcttcctga
      661 caggaggccg ttttgttttg tagcccgcct caacgcactt atttagtgca tccatctgct
      721 atccccagct ggttaagtaa attttttgta tccacatcat cacacaatcg ttacataaag
      781 attgtttttt catcaggttt tacgctaaat aatcactgtg ttgagtgcac aattttagcg
      841 caccagtttg gtgccccaga atggtgcatc ttcagggtat tgccctataa atcgtgcatc
      901 acgtttttgc cgcatctcga aaaatcaatg agttgcaaaa ctggcacgat tttttcatat
      961 atgtgaatgt cacgcagggg atcgtcccgt ggatagaaaa aaggaaatgc tatgaagtct
     1021 gtattaaaag tttcactggc tgcactgacc ctggcttttg cggtttcttc tcatgccgcg
     1081 gataaaaaat tagttgtcgc gacggatacc gccttcgttc cgtttgaatt taaacagggc
     1141 gataaatacg tgggctttga cgttgatctg tgggctgcca tcgctaaaga actgaagctg
     1201 gattacgaac tgaagccgat ggatttcagt gggatcattc cggcactgca aaccaaaaac
     1261 gtcgatctgg cgctggcggg cattaccatc accgacgagc gtaaaaaagc gatcgatttc
     1321 tctgacggct actacaaaag cggcctgtta gtgatggtga aagctaacaa taacgatgtg
     1381 aaaagcgtga aagatctcga cgggaaagtg gttgctgtga agagcggcac tggctccgtt
     1441 gattacgcga aagcaaacat caaaactaaa gatctgcgtc agttcccgaa catcgataac
     1501 gcctatatgg aactgggcac caaccgcgca gacgccgttc tgcacgatac gccaaacatt
     1561 ctgtacttca tcaaaaccgc cggtaacggt cagttcaaag cggtaggtga ctctctggaa
     1621 gcacagcaat acggtattgc gttcccgaaa ggtagcgacg agctgcgtga caaagtcaac
     1681 ggcgcgttga aaaccctgcg cgagaacgga acttacaacg aaatctacaa aaaatggttc
     1741 ggtactgaac cgaaataata acgctgcgcc tgtaaagcgc actggcagtt ccctctcccc
     1801 gccggggaga ggattagggt gaagggcgca aacccgctcc ggggccatta attaccctga
     1861 atttgattat ttacaccacg gtaacaggaa caacatatgc agtttgactg gagtgccatc
     1921 tggcctgcca ttccgcttct gattgaaggt gccaaaatga ccctgtggat ttcggtcctc
     1981 ggtctggctg gcggtctggt gatcggattg ttggcaggtt ttgcacgcac cttcggtggt
     2041 tggatagcca accacgtcgc gctggtcttt attgaagtga tccgcggcac acctatcgtc
     2101 gtccaggtga tgtttatcta tttcgccctg ccgatggcgt ttaacgattt acgcatcgac
     2161 ccgtttactg cggcggtggt caccatcatg atcaactccg gcgcgtacat tgcggaaatc
     2221 acgcgtggtg cggtgctgtc tattcacaaa ggttttcgtg aagcaggact ggcgctcggt
     2281 ctttcacgtt gggaaaccat tcgctacgtc attttaccgc tggcactgcg tcgtatgctg
     2341 ccgccgctgg gtaaccagtg gatcatcagc attaaagaca cttcgctgtt tattgttatc
     2401 ggcgtggcgg aactgacccg tcaggggcaa gaaattattg ccggtaactt ccgcgccctt
     2461 gagatctgga gcgccgtggc ggtgttctat ctgattatta ccctggtgct gagctttatt
     2521 ctgcgtcgtc tggaaagaag gatgaaaatc ctgtgattga atttaaaaac gtctccaagc
     2581 attttggtcc aacccaggtg ctgcacaata tcgatttgaa cattgctcag ggcgaagtcg
     2641 tggtgattat cgggccgtcc ggttccggta aatcgaccct gctgcgctgc atcaacaaac
     2701 tggaagaaat cacctccggc gatctgattg tcgatggcct gaaggttaac gatccgaaag
     2761 ttgatgagcg cctgattcgc caggaagcgg gtatggtgtt ccagcagttt tacctcttcc
     2821 cgcatctgac ggcgctggaa aacgtcatgt ttggcccgct acgcgtgcgt ggcgcgaata
     2881 aagaagaggc ggaaaaactg gcgcgtgagc tgctggcgaa agtcggtctg gcagagcgtg
     2941 cacagcacta cccttccgaa ctttctggtg gtcaacagca gcgtgtggcg attgcccgcg
     3001 cgctggcggt gaagccgaaa atgatgctgt ttgatgaacc gacttccgct cttgacccgg
     3061 aactgcgcca tgaagtgctg aaggttatgc aggatctggc tgaagaaggg atgacgatgg
     3121 tgatcgtgac ccacgaaatc ggttttgccg agaaagtagc ttcgcgcctg atctttatcg
     3181 acaaaggccg gattgcagaa gatggcgatc cgcaggtgtt gatcaagaac ccgccgagcc
     3241 agcgcttgca ggaatttttg cagcacgtct cttaacttct ctgcctccct ttcccccgaa
     3301 agggaggcac accagattcc tctcatttaa aattgcctct cccccagcat ctatacttat
     3361 ctttttgctc tgttttctca ctggaggagt catgcggtgg atcctgttca tcctcttctg
     3421 cctgctgggc gcacctgccc acgcggtatc catacccggc gttacaacca caacgacaac
     3481 ggactcaacg actgaaccgg ccccggaacc ggatatcgaa caaaaaaaag cggcctatgg
     3541 cgcactggcg gatgtgctgg ataatgacac ctcgcgtaaa gagttgatcg accagttgcg
     3601 caccgtcgcc gcgacgcccc ctgcggaacc ggtaccgaag atcgtgccgc cgacgctggt
     3661 tgaagagcaa accgtgctgc aaaaggtcac cgaagtcagc cgccattatg gtgaagccct
     3721 ttccgcccgc ttcgggcaac tttatcgcaa tatcaccggc tccccgcata agccgtttaa
     3781 tccacaaacc ttcagcaatg cgctgaccca cttttcaatg ttagcggtat tagtgtttgg
     3841 tttttactgg ctgattcgtc tgtgcgcact gccgctgtat tgcaaaatgg gccagtgggc
     3901 gcggcaaaaa aatcgtgagc gcagtaactg gttgcagctt ccggcgatga tcatcggggc
     3961 gtttattatc gacctgctgt tactggcact gacattattt gtcggccagg tattaagcga
     4021 caacctgaat gcgggcagtc gcaccatcgc tttccaacaa agtttgtttc tcaacgcctt
     4081 tgccctgatt gaatttttca aaaccgtact acgcctgatt ttttgcccaa acgtagctga
     4141 actgcgcccg ttcacgattc atgacgagac cgcccgttac tggagtcgtc gcctgagctg
     4201 gttaagcagc ctgattggct atggtctgat tgtggctgtg ccgattatct ctaatcaggt
     4261 gaatgtacag ataggtgcgc tggcgaacgt catcattatg ctgtgcatga ccgtctgggc
     4321 gttgtacctg atttttcgta ataaaaaaga gatcacccag catttgctca actttgcgga
     4381 gcattcgctg gcctttttca gcctgtttat ccgcgccttt gcactggtgt ggcactggct
     4441 ggcaagcgcc tattttatcg tgctgttttt cttttcgttg ttcgatccgg gcaacagcct
     4501 gaaatttatg atgggtgcaa cggtgcgcag cctggcgatt attggtatcg cagcatttgt
     4561 ttccggtatg ttttcccgct ggctggcgaa aaccatcacc ctctcgccac atactcagcg
     4621 aaattatccg gagctgcaaa aacggctgaa tggatggctt tcggcggcac tgaaaacagc
     4681 gcgtattctg acagtctgcg tggcggtaat gctgctgttg agcgcatggg gattgttcga
     4741 tttctggaac tggctgcaaa acggtgcggg gcagaaaacc gtagatatcc tgatccgtat
     4801 cgcactcatt cttttcttct cggcggttgg ctggacggtg ctcgccagtt tgatagaaaa
     4861 ccggctggct tcggatattc atggccgccc gctacccagc gcccgtacgc gtaccctgct
     4921 aacgctgttc cgtaacgcgc tggcggtgat tatcagtacc atcaccatca tgattgtgtt
     4981 gtcggaaatc ggcgtcaata tcgcgccatt gctggcaggt gccggtgcat tgggactggc
     5041 tatctctttt ggttcgcaaa cgctggtgaa agatattatc accggggtat ttattcagtt
     5101 tgaaaacggc atgaacactg gagatttggt gactatcggg ccgttgaccg gcactgtgga
     5161 acggatgtca attcgctccg tgggcgtgcg ccaggatacc ggggcgtatc acatcattcc
     5221 gtggtcttcg ataaccacct ttgctaactt cgtccgcggt attggttcgg tagtggcaaa
     5281 ctacgatgtt gatcgccatg aagatgctga taaagccaat caggcactga aagatgcggt
     5341 agcagaattg atggaaaacg aagaaattcg cgggctggtt attggtgaac cgaattttgc
     5401 cgggattgtc ggcttaagca ataccgcgtt tacactgcgt gtttcgttca ccacgctgcc
     5461 actcaaacag tggacggtac gctttgccct cgacagccag gtgaaaaaac atttcgacct
     5521 ggcgggtgtt cgcgcgccgg tgcagactta tcaggtgctg ccagctccgg gcgcggcccc
     5581 ggctgaaccg ttgccgccgg gggagccaac gctttaacgc tggcgattga caaaacggcg
     5641 gcgctgctcg tcgttcataa aggtccaggc tataaagcgg ctctgctttt gcccctgggc
     5701 catctctttt tttaccacct tcaccgcacc tacgtccgtc aaagcacgat acaacggcgg
     5761 taagttttca ccacgagata ccagcgaggt aaaccacatc acctgcttcg cgaagccttt
     5821 gctctcttca atcatctttt tgataaaagc gacctcaccg ccttcacacc acaactcctg
     5881 ttgctgacca ccaaagttca gtgcatcgtc tttgttcagc cccaggttac ggcgtttacg
     5941 ttcactacct gcccgtgccg cagcggcgga atcgtggaat ggcgggttac acaaggtcgc
     6001 atcgtattgc tcgtttttat ggatgatgcc gttaaaaatc gccccactct ctttttgccg
     6061 acgcagacga atggcgcggt taagccccgg attagcactg atgatcgcct gcgcactgct
     6121 taacgcctgg ctgctggttt cactaccggt aaaacgccag ccatattcat gtacgccaat
     6181 cagcggataa atacagttcg caccaacgcc gatatccaga atgctggcat tagctggaat
     6241 tgttccgctt gcttcggcca gtaaatcggc aaggtgatga atataatccg cccggcccgg
     6301 tactggtggg cagagaaaac cgtccgggat atcccagttc gctacggcgt aaaaatgggc
     6361 cagcaacgcc ttattgagcg ccttcaccgc cagcggattg gcaaagtcta cgctttgctc
     6421 cccggcgggt gtaagcgtga ggaattgcct gagttcagga ttgacctgac aaagcgtggc
     6481 gagatcgtag cggctgtgat gacggttgcg cggatgcaac cccggtttct gggcggacat
     6541 agcattctcc tttaagcagc ggcgtaagat acccgcataa cgcgacaggg taaatatctg
     6601 gctgcgttta tcatttctaa aaaccttcac atggctatgg tttatggcaa ttcaccataa
     6661 aaaacatcat catgcatgtc attgatttgt cttattttgt aaaaacacat cgttcaaaaa
     6721 ataagcgcat ctctgtcaat tttttgcgtc attttcatcg cgcaggcgac ctacacttat
     6781 ctggcactac ccgttgtatt tatgaggatg tcattatgaa aaagtgcctc acactactga
     6841 ttgccaccgt cctgagcgga atctctctca cggcttatgc cgcgcaaccg atgagtaacc
     6901 tggacagtgg tcaactacgg cccgccggta ccgtttcggc aactggcgca tcaaacctaa
     6961 gcgatctgga ggataaactg gcagaaaaag cgcgcgaaca aggcgcgaaa ggttatgtca
     7021 ttaattccgc aggcggaaat gaccagatgt tcggtactgc aatcatctac aaataacccc
     7081 accaacgtaa ctgcattcgc ctctggatgc agttttcgtc caatttgtaa gcaaaaaaca
     7141 tcaaatatga acgcaatgta aataaatgta tttctttttc gcgcaatggg tgatagaaaa
     7201 tcgcgccaag tgataatgct tatcaatatt attatcactt gcacgtacgc tatcacggga
     7261 ttaacagtgg catcgcatcc gcagagatgc tttctcgtga cagtgaaaat ttcaccatat
     7321 aagaaaaagt cacctgcaaa atggaaaaca atcgcaattt ccctgccaga caatttcatt
     7381 cgctcacgtt ctttgccggt ctttgtattg gtattacgcc tgtggcacag gcactcgctg
     7441 ccgaagggca agctaacgcg gatgacacgc tggttgtcga agcatcaacg ccttcgcttt
     7501 atgcgccaca aaaatctgcc gatccgaaat tctcgcgtcc ggtagcggat actacccgca
     7561 cgatgacagt gatttctgaa caagtgatta aagatcaggg tgcaaccaac cttaccgacg
     7621 cgctcaaaaa cgtccccggc gtgggtgcgt tttttgcggg tgagaacggt aactccacca
     7681 ctggcgacgc catttatatg cgcggtgccg atacctctaa cagtatttat atcgatggca
     7741 ttcgcgatat cggcagcgtc tcgcgcgaca ccttcaatac cgagcaggtc gaagtaatta
     7801 aagggccgtc cggcaccgac tacgggcgca gcgcaccgac aggctcgatc aatatgatca
     7861 gcaaacagcc gcgcaatgat tccggcattg acgcctccgc cagtattggc agcgcctggt
     7921 tccgccgcgg cacgctggac gtcaatcagg tcattggtga taccactgcg gtgcgcctga
     7981 atgtaatggg cgaaaaaacg cacgatgccg gacgcgacaa agtcaaaaat gagcgttacg
     8041 gcgtcgcccc ttctgtcgct tttggccttg gtacagcgaa tcgtttgtat cttaattatc
     8101 tgcatgtcac ccagcacaac acgccagacg gcggcattcc gaccatcggt ttaccgggtt
     8161 tttctgcccc atctgcggga acagcggccc tgaatcattc cggaaaagtt gatacgcata
     8221 acttttacgg cacggattcc gattacgacg attcgaccac cgacaccgct accatgcgtt
     8281 ttgagcacga catcaacgat aacaccacca ttcgcaacac tacgcgttgg tcgcgcgtaa
     8341 agcaggatta cctgatgacg gcgattatgg gtggagcgtc gaatattacc cagcccacca
     8401 gcgatgtgaa tagctggacg tggtcacgca cggcgaatac caaagatgtg agtaacaaaa
     8461 ttctcaccaa ccagaccaac ctgacctcga cgttctatac cggcgctatc ggtcatgatg
     8521 tcagtaccgg cgtggaattt acccgcgaaa cacagactaa ctatggcgtt aatccggtga
     8581 cgttaccgac ggtaaatatt tatcatcctg acagcagcat tcatcccggc ggcctgacgc
     8641 gcaacggcgc aaacgccaat ggtcagacgg ataccttcgc aatttatgcc ttcgatacgc
     8701 tgcaaatcac ccgtgatttt gagctgaacg gtgggatccg tctggataat tatcatactg
     8761 aatatgacag tgccaccgcc tgcggcggca gcgggcgcgg tgccatcacc tgccctgctg
     8821 gcgtggcaaa aggttctccg gtaaccaccg tcgacaccgc caagtcgggc aatctggtga
     8881 actggaaagc cggggcgctg tatcacctga cggaaaacgg caatgtttat attaactatg
     8941 ccgtttccca gcagccgccg ggcggcaaca acttcgccct tgcgcagtct ggcagcggta
     9001 acagtgccaa ccgcaccgat tttaaaccgc aaaaagccaa caccagcgag attggcacca
     9061 aatggcaggt tctggataaa cgcctgctac ttaccgccgc gctgttccgt actgatattg
     9121 aaaatgaagt tgagcaaaat gatgatggga cttattcgca atatggtaag aaacgtgtcg
     9181 aaggctatga gatatccgtg gccgggaata tcacccccgc gtggcagatg attggcggct
     9241 atacccagca aaaagcaact atcaaaaacg gcaaagatgt tgcccaggat ggttcctcat
     9301 cgctgccgta taccccggaa cacgccttca ccttatggag ccagtatcag gcaaccgatg
     9361 atatttctgt tggtgcgggc gcacggtata tcggcagtat gcataaaggt tcagacggcg
     9421 cggtgggaac gccagcattt accgaaggtt attgggtcgc cgacgccaaa ctggggtatc
     9481 gggttaatcg caatctcgac ttccagctaa acgtctacaa cctgtttgat accgattacg
     9541 tcgcctcaat caataagagc ggctaccgtt atcacccggg cgagccaaga accttcttgc
     9601 tcacagccaa tatgcatttc tgattcagat gtggggcgca ggccccactt tttggagaac
     9661 ttgtatgatg taccacattc ccggcgtgtt atcgccacag gacgtcgccc gttttcgcga
     9721 acacctggaa caagccgaat gggtggatgg acgcgtcacc accggcgcac aaggtgcgca
     9781 agttaagaac aatcaacaag tagacacccg cagcgccctg tacgccgccc tgcaaaatga
     9841 ggtgctgaac gcggttaacc aacatgcttt gttctttgcc gcggccttgc cgcgtaccct
     9901 ttccacgccg ctgtttaatc gctatcagaa caatgaaacc tatggttttc atgtggatgg
     9961 cgcagtacgc agccatccac aaaacggctg gatgcgcact gacctttctg ccacgctgtt
    10021 tttaagcgat ccagaaagct acgacggcgg cgaactggtc gttaatgaca ccttcgggca
    10081 acatcgggta aaactcccgg caggcgatct ggtgttgtat ccctccagca gcctgcattg
    10141 cgtgacaccc gtaacccgcg gcgtgcgagt ggcatcattt atgtggatcc agtcgatgat
    10201 ccgcgatgat aaaaagcgcg ccatgctgtt tgaactggac aacaatattc agtcgctgaa
    10261 aagccactac ggcgaaagtg aagagatcct gtcgctgctc aatctttatc ataatctgct
    10321 gcgggaatgg tcggagatct gacgcttgaa ctgcccgagt tcgtccctat attagctaca
    10381 cttaactgta caagcattga tatggggagg acgatatggc atccggttgg gctaatgatg
    10441 acgccgtcaa cgaacagatc aacagtacaa ttgaagatgc gattgcccgc gctcggggtg
    10501 aaattccgcg tggcgaaagc ctgtatgaat gtgaagagtg cggtgcccct atcccgcagg
    10561 cccgtcggga agccattcct ggcgtgcgct tatgcattca ttgtcagcag gagaaagatt
    10621 tacaaaaacc agcttataca ggatataatc gcagaggttc gaaagacagc cagttacgtt
    10681 aactacaact ggcgggatgt atagcgaata taatttgtat tttgcgtggc attccgtgcc
    10741 tttaacgcta ccgtgactgc cacgcggtat aaaacaagtc cataagtaca acaaataaat
    10801 ggtttatcag tatgttagat atcaatcaat ttatttgaac aaggtggtca attctcttcg
    10861 attttatctc tcgtaaaaaa acgtgatact catcacatcg acgaaacaac gtcacttata
    10921 caaaaatcac ctgcgagaga ttaattatga aaactatcaa tactgttgtt gctgctatgg
    10981 ctctttcaac tctgtcattt ggcgtgttcg cggcggaacc ggtaacggca tcccaggcac
    11041 agaacatgaa taaaatcggc gtggtttctg ccgatggcgc atccaccctc gatgccctgg
    11101 aagcgaaact ggctgagaaa gccgcggcgg ccggtgccag tggatacagc atcacttctg
    11161 ccaccaacaa caataaatta agcggtactg cggtaattta taagtaattc gtaagctgga
    11221 tcaggcgttt acgctacgtt cagcacaagt atttccctgt ctgtttgccg acagacgcat
    11281 atgctctaac cctcatgatc ctatgttacc cttgtttgcc cgtccgccac tggacgggct
    11341 ttttttagct ggctagctgc tgacagaaag cctgcaacgt ctcttccggc ataccaatct
    11401 gccgcgccgc atcacaaatg gcctgccagc ttcccgcatc cagtggaatc ccctgctcct
    11461 gccgttcgcg acgcgtgttc acttcccact cgcccggtag caaaatcggc ttatcgtcat
    11521 catgcggcga ggctttcacc cactcggcaa aggcttcggt ctgcgcacta caatccggcg
    11581 cgccgaacag ttccgggttg ataatgatag tggtcatgca gttaagaatg gcatcgggac
    11641 tggtttgtaa cgtttcctga tgcgtcgttt taccgccaga aagcgccccg ccaagaattt
    11701 cacacatcgc tgcaagggcg tagcctttat gttcggcaaa ggtcaacagc gaacccaacg
    11761 gcgactcctg cattaccgcc ggatttgtcg tcggcacgcc gttaacgtca atcaggcaac
    11821 ctggcggcac ggggacgcct ttatgccagg cgacgcgggt tttgccaaat gcaatggcgc
    11881 tggtggcgta atcgagcaac agcgggaaat tatctttacg agggaaaacc acacagaacg
    11941 gattggtgcc aaagcggctg tcgcgaccgt ggaacggcgc gaccattgga ataccgacca
    12001 cgctaacaaa gtggatagag acaaaccccg ccgctgcaca ctgctccgcc cagtaaccga
    12061 tacggccgat atgatgcgag ttatgtagcg ccacggcggc aatgccgtgc tgatgcgctt
    12121 tctcaatccc cagcgccatc gcttcgtgtg ccgcgacctg accaaatgcg cgatcgccat
    12181 cgagcgtgac cgccgccccc gcttctttaa cgactttggc atgatggtta atttgcagat
    12241 gcccctgact ccaggagcgc acatagcttg ggatcatgcc aataccatgt gaatcatgcc
    12301 ctgccaggtt tgccgcgatc aaatgatcgg cgactaattt cgcttcctgt tcctcactac
    12361 ccatctgacg aaatacagcc tgaataaaac tgtgcagcgt ctgagcatca aagcgatggc
    12421 cactttccat ggttaactcc tgtttatgtt atgtgtttgt tgttttttat gctgcacgcc
    12481 gggcgcggga aggtcaataa agatgcagga atttcatatt gcggtggggt aaaagccgga
    12541 tgacatggct catccggctg agataaaaat taaaatgcct gattaacgcg cgccaggccg
    12601 tcgctgatag ttgccgcttc acccgtagcc atcaggcagc aagccatctg gattttcagc
    12661 gattcgggaa tcggttcgct gccagccagg caacgctcaa tccactgcgc cgtggtttcc
    12721 ggatcttttg cttgtggcag taactcgcta ccagcagtgt cctgtttttc atacagtacc
    12781 cgcatccctt cacggtcaat gagattgatc tgcgggcagc gctgcggatt agcatacact
    12841 tcaccttcag taccatgcat aagtagcgcc cgcccgccga tatcgctaaa gaacttcgcg
    12901 acgcgtccaa tgtattccgg atgcgaaacg ctggaaagac gcagtgcctc accttcggca
    12961 aatggcgtcg ccagtttcgc cagggtatgt gcactgttac gcacgcccat ccgccagcgc
    13021 atcgccagtt gtttttccag cggcgggcaa aacgcgccga caggcataaa gacaggttga
    13081 tgttcgtcga gcttcgcctg cgcctgcccg ccgtgcaacg ttggcgtaat acccatcagt
    13141 tcgaaaatgg tttcggtcag cacgcgggtt ggatcttcgc taaccccgtg aaccaccaca
    13201 ggaaaaccga gtttgtgcag cagaatcgcc agcaacggcg tcaggttggc ctgtttacgc
    13261 gcgccgttgt aactggggat gacaatcggc atcggcttgc ctgcaggcgg cgtcagcttg
    13321 atggtgtgat tttgcatggc ttcgtaaaag ccgagcatct ctgcttcccc ttcccctttg
    13381 atacgtagcg caatcaatac gccgcccaac tcaaggtcag ggacttcacc attgagcata
    13441 tgggcataca gaccgcgcgc agtatcccgg tctaaatcgc gcgcgtggtt tttcccgcgc
    13501 ccgatctctt taatgatttt gcgatagtcc atttacgact ccttacctga ctcacatcat
    13561 taacgccgcc gacggcgtgg ggatttcgtt ttttctttct ttttaactat accttcaggg
    13621 acttccggtt gctctatcgg aaataccggt aatgcatcca gtagtcgctt gccataattt
    13681 tttgtcagca agcgtttgtc gtagataacc acttcccccc agcaaccgtg gcttcgaatc
    13741 agtcgcccaa cctgctgaat caggttaaac gaagcgctcg gcaggctttg cacctcaaac
    13801 ggatagcggt taaggctttt cagccattca ccttcggtga tcaccaccgg actgtcgatg
    13861 ggcggaaaag cgattttgtg gatatgcacc tggctgagca gagcgccttt cagatcaaga
    13921 ccttcggcaa aggactgtaa gcccaccagc acgctacgct caccgttggc aacgcgtttg
    13981 cggtgcagtt caactaagcg ataacgtggc tgatcgccct gcaccagcaa catcaggcgt
    14041 aaatccgtca catagtcgag aaagcgctgc atcgcacgcc cgctggcaaa cagcaccaac
    14101 ataccgagat gttttttgct ctccacctgc tcacggaaaa aggccgccat ttcagcaatg
    14161 tgctgttctt cgttgtcgat ggaaggctca acgcgcatcc ggggaataac aattttgccc
    14221 tgttcgcagt ggttaaaggg cgaatccagc gccacaaagc ggtcacccgc tttctctttc
    14281 agcccactca tctcctgcaa acgcgaaaaa ctgttcagcg aacgcaaggt tgcggaggta
    14341 acaataatgt gcggaatact gcgccacagc agcctttcca gttggtcgct aacacgaata
    14401 cccacacagt gaaaccacag atgtagctgc ccttcgcgct cttcccgcgt cgcccatttg
    14461 gtcaccggcg caccggaaga ttgcgccaga gacgccagcc gccagagttt gctttgcgcc
    14521 tcgaacatcc ccaacgcgcg gttcatctgc aaaatcaacc gatgcaggcg tacaatgtca
    14581 tggctgccgg ttttctcact taaatcgtta agaaataact ccgccagacc acgcagcatc
    14641 tcggtgagtt ttgccagccg ctggcagatc tccagcactt catcaggcag ttcgcccatc
    14701 gcaaaacggt gctctgcctc ctgcccggca ggcatgtaga gattgagaat gttgtttaac
    14761 gaggcgataa gctcatacag ctcttcacaa tgcgcattca aacgttcagg gatcgccagc
    14821 ggtgggatgg tcttcgggcg aaactgctcc atgcaggtgg cgaccagttt ggtgaacagg
    14881 tccagttgga gccggtacca gggcgcagtg atttcggcgc tcatctccag cgcatcccgc
    14941 gccacatccg gcaggtgatg accttcgtcc agcaccagca gtaaattttt cgggtcaggc
    15001 aataccgctt cgctttccat tgccgccatc accagcgcat ggtttgccac caccacttcc
    15061 gcttcctgaa tttcccgacg agcgacaaaa aacgggcatt cgcgatagta gtaacagttg
    15121 cggttgaggc agctggcttt gtcggtactc aaacgacgcc agagatcatc atctatagcg
    15181 atatcagtat gatcgcgcag gccatcccat ttataagtgt cgagatcgcc cttcagcttc
    15241 gcacaacgtt tttgctcttc ctgattgttc ggcgtcagtt cgtcgtcaag aaacgccagc
    15301 agatcctgtt gcgtgggctc agtgctggcg agcgctgtca gattacgcgg gcaaacgtag
    15361 cgcccacgcc caaaagcggc agtgaattta agatcgggaa tgatcttttt cagcagcggt
    15421 aaatctttgc tgtagatctg atcctgcaat gccacgttgg cggtacttac caccagcgtt
    15481 ttttgctctt cgcgggcaat ggcgatgccg gggatcaaat aggagagcgt tttcccaacg
    15541 ccggtaggag cttcaatcgc cagatgccgc ccttcttctc cggccagcgt tttggcgacg
    15601 tccgcaatca tctgccgctg cggcgcacgg ggaataaagt cggggatctg ttcctgaagc
    15661 gccttatacc aggcggcaat ttgcgcttta agcgcggcgg ttaatgccat gagaaaacct
    15721 gaaatactgt ataaacagcc aatattgtgg catttttcgg ttttcagggg taatggtttt
    15781 tctgtttctg gaaagcggct cgcaataacc agcaggatga tgcgtaaagc gcttcgcgct
    15841 aaattcgaac aacatgcaga actgcgtaca ctcttgctgg caaccgcgtc cgcaaaactg
    15901 gttgagcata cgcaaaatga tgcttactgg ggagatggcg gtaatggtca gggcaagaat
    15961 cgcctggggt accttttaat ggcgttgcgc ggacaattgg ctgcggagaa gtaacgcatc
    16021 gggtggcttg tgggcgttaa aacagagaaa tctttttgcc gcgctcgcaa atggctgaca
    16081 agtgcttgtt gtgaaatgcc ggatgcggcg cgagcgcctt atccggccta caaaaacatg
    16141 cacattcaat atattgcaga gttcacgtag gcctgataag cgtagcgtat caggcagttt
    16201 tgtgtttgtc atcagtctca tgccgggcat agcccggcat aaaagattac tgcgcagcgg
    16261 caggtttacg cggacggcgg cgacgttgtt gttcgcctgc cggtttggca tcgccgagac
    16321 gacgagacgg tttttctgcg ggcttcgcgt taccagactt cgcaccactt tccgcacggc
    16381 gtggttgctg ttgaccgcga ccaccgccct gtccacgacc gccgccacgt tgctggcgac
    16441 cgttttggat cggttcggct ttgattgacg ggtccggctc atagcccaga atcgcaatgc
    16501 gcgggatctc ttttttcagc agtttttcga tatcacgcag cagtttgtgt tcatcaacac
    16561 acaccagcga caatgcttcg ccagttgccg ccgcacggcc agtacgcccg atacggtgaa
    16621 cataatcttc cggtacgttt ggtaactcat agttgacgac gtgcggcaac tcttcgatat
    16681 ccagaccacg cgcagcgatg tcagttgcca ccagtacacg aatatcgccc gatttaaaat
    16741 cggccagcgc acgagtacgc gcaccttgcg atttattacc gtggatcgcc gcactacgga
    16801 tgccatcttt attaagctgt tcagccagat ggttagcgcc atgtttggta cgggtaaaca
    16861 ccagcacctg ctgccagttc cctttcccaa tcatatgcga cagcaattcg cgtttgcgtt
    16921 tcttatcgac aaagtgaacg tgctgggtca cctgatcaga cgcagtattg cggcgtgcca
    16981 cttcgatttc cagcgggttg tgcaacagtt tttccgccag ggctttaata tcgtcagaga
    17041 aggtcgcgga gaacaacagg ttctggcgct tcgctggcag ttttgttaac acgcgacgga
    17101 tatcgtggat aaagcccatg tcgagcatgc ggtcagcttc atcaaggacg aggatttcaa
    17161 cctgatccag cttcactgca ttttgatgtt ccaggtccag cagacgtccc ggcgttgcca
    17221 ccagcacatc aacgccgccg cgcagcttca tcatctgcgg gttaatgctg acaccgccaa
    17281 acaccaccag cgaacgaatg ttcaggtatt tgctgtaatc acggacgttt tcgccaatct
    17341 gcgccgccag ttcacgggtc ggggtaagaa tgagcgcacg caccggacga cgccctttgg
    17401 cgtgcggctg gcgagtgatc aggtgttgca acagcggcag cgtaaagccc gctgttttac
    17461 ctgtaccggt ctgggcgctg gccatcaggt cgcggccttc cagcaccgca gggatcgcct
    17521 gctgctgaat aggggtgggt tcacggtaac cctgctcggc aacggcgcgc aggatatcag
    17581 ggcttaaacc caaagaatcg aaagacataa ctactccgaa ccgccccgac cgttaccggt
    17641 gtagttttca gggagatact gaaaaggaaa atgacaaaaa ccacaatgtc atgagggagc
    17701 ggagtgtagc agtttttgtg acgcagcgca taaattatcc ctgtgactgg cgtagctaaa
    17761 attcttagcc atactggaca actcccctat ggagtcataa tcttaatcaa tcatttgatt
    17821 aagaatggac tgtgcgatga ataatcctgc catgacaatc aagggtgaac aggcgaaaaa
    17881 acagctgatt gctgcggcac tggcacagtt tggtgaatat ggaatgaacg ccaccacccg
    17941 cgagatagcc gcccaggccg ggcagaatat cgctgccatc acctactact tcggttcgaa
    18001 agaagatttg tacctcgcct gcgcccagtg gattgctgat tttattggcg agcagttccg
    18061 tccgcacgcc gaagaagccg aacgtttgtt cgcgcaacca cagccggatc gggctgccat
    18121 ccgtgaactg atccttcgcg cctgcagaaa catgattaag ctgctcaccc aggatgacac
    18181 cgtcaacctc agcaaattta tctcccgtga gcagctctct cccacggcag cctaccacct
    18241 ggtgcacgaa caggtgatta gtccgctaca cagtcacctt acacgcctga ttgccgcctg
    18301 gactggctgc gacgctagcg atacccgcat gatccttcat acccatgcgt tgattggtga
    18361 gattctggcg tttcgtcttg gcaaagaaac tattctgtta cgtaccggct ggaccgcgtt
    18421 cgatgaagaa aagaccgaac tgatcaacca gacggtgacc tgtcatattg acctgatttt
    18481 gcaaggatta tcgcaaagga gtttgtagtg atgaaaaaac ctgtcgtgat cggactggcg
    18541 gtagtagtac ttgccgccgt ggttgccgga ggctactggt ggtatcaaag ccgccaggat
    18601 aacggcctga cgctgtatgg caacgtggat attcgtacgg tgaatcttag tttccgtgtt
    18661 ggggggcgcg ttgagtcgct ggcggtggac gaaggtgatg cgatcaaagc gggccaggtg
    18721 ctgggcgaac tggatcacaa gccgtatgag attgccctga tgcaggcgaa agcgggtgtt
    18781 tcggtggctc aggcgcagta tgacctgatg cttgccgggt atcgagatga agaaatcgct
    18841 caggccgccg cagcggtgaa acaggcgcaa gctgcctatg actatgcgca gaacttctat
    18901 aaccgccagc aaggactgtg gaaaagccgc accatttcgg caaatgacct agaaaatgcc
    18961 cgctcctcgc gcgaccaggc gcaggccacg ctgaaatcag cgcaggataa attgcgtcag
    19021 taccgttccg gtaaccgcga acaggacatc gcccaggcga aagccagcct cgaacaagca
    19081 caggcgcaac tggcgcaggc ggagctgaat ttgcaggact caacgttgat agcaccgtct
    19141 gatggcacgc tgttaacgcg cgctgtagag ccaggcacgg tgcttaatga aggtggcacg
    19201 gtatttaccg tctcgctgac gcgtccggtg tgggtacgcg cctatgttga tgaacgtaat
    19261 cttgaccagg cccagccggg gcgcaaagtg ttgctttata ccgatggtcg cccggacaag
    19321 ccgtatcacg ggcagattgg tttcgtttcg ccgactgctg aatttacccc gaaaaccgtc
    19381 gaaacaccgg atctgcgtac cgacctcgtc tatcgcctgc gtattgtggt gaccgacgcc
    19441 gatgatgcgt tacgccaggg aatgccggta acggtgcaat tcggtgacga ggcaggacat
    19501 gaatgatgcc gttatcacgc tgaacggcct ggaaaaacgc tttccgggca tggacaagcc
    19561 cgccgtcgca ccgctcgact gcaccattca cgccggttat gtgacggggc tggttgggcc
    19621 ggacggtgcg ggcaaaacca cgctgatgcg gatgctggcg ggattactga aacccgacag
    19681 cggcagtgcc acggtgattg gctttgatcc gatcaaaaac gacggcgcgc tgcacgccgt
    19741 gctcggctat atgccgcaga aattcggcct gtatgaagat ctgacggtga tggagaacct
    19801 caatctgtac gcggatttgc gcagcgtcac cggcgaggca cggaagcaaa cttttgctcg
    19861 cctgctggag tttacgtctc ttggcccgtt taccggacgc ctggcgggca agctctccgg
    19921 tgggatgaaa caaaaacttg gcctggcctg taccctggtg ggcgaaccga aagtgttgct
    19981 gctcgatgaa cccggcgtcg gcgttgaccc tatctcacgg cgcgaactgt ggcagatggt
    20041 gcatgagctg gcgggcgaag ggatgttaat cctctggagt acctcgtatc tcgacgaagc
    20101 cgagcagtgc cgtgacgtgt tactgatgaa cgaaggcgag ctgctgtatc agggagaacc
    20161 gacggccctg actcaaacca tggccggacg cagctttctg atgaccagcc cgcacgaggg
    20221 caaccgcaaa ctgttgcaac gggcattgaa actgccgcag gtcagcgacg gcatgattca
    20281 ggggaaatcg gtacgtctga tcctcaaaaa agagaccaca ccagacgata ttcgccatgc
    20341 cgacgggatg ccggaaatca acattaacga aactacgcca cgttttgaag atgcgtttat
    20401 tgatttgctg ggcggtgccg gaacctcgga atcgccgctg ggcgcaatat tgcatacggt
    20461 ggaaggtaca cctggcgaga cggtgatcga agcgaaagag ctgaccaaaa agtttggcga
    20521 ttttgccgcc accgatcacg tcaactttgc cgtcaaacgc ggtgaaatct ttggtttgct
    20581 cgggccaaac ggcgcaggga aatcgaccac ctttaagatg atgtgcggtt tgctggtacc
    20641 gacctctggt caggcgctgg tgctgggcat ggatctgaaa gagagttccg gtaaagcgcg
    20701 ccagcatctc ggctatatgg cgcaaaaatt ttcgctctac ggcaacctga cggtcgaaca
    20761 gaatttacgc tttttctctg gtgtgtatgg cttacgcggt cgggcgcaga acgaaaaaat
    20821 ctcccgtatg agcgaggcgt tcggcctgaa aagtatcgcc tcccacgcga ccgatgaact
    20881 gccattaggt tttaaacagc ggctggcgct ggcctgttcg ctgatgcatg aaccggacat
    20941 tctgtttctc gacgaaccga cgtccggcgt tgatcccctc acccgccgtg aattttggct
    21001 acatatcaac agtatggtag agaaaggcgt cacggtgatg gtcaccaccc actttatgga
    21061 tgaagcggaa tattgcgacc gcatcggcct ggtgtaccgc gggaaattaa tcgccagcgg
    21121 cacaccggac gatttgaaag cgcagtcggc caacgatgag caacccgatc ccacgatgga
    21181 gcaagccttt attcagttga tccacgactg ggataaggag catagcaatg agtaatccga
    21241 tcctgtcctg gcgtcgcgtg cgggcgctgt gcgtcaaaga gacgcggcag atcgttcgcg
    21301 atccgagtag ctggctgatt gcagtagtga tcccactgct actgctgttt atttttggat
    21361 acggcattaa cctcgactcc agcaagctgc gcgtcgggat tttactggaa caacgtagcg
    21421 aagcggcgct ggatttcacc cacaccatga ccggttcgcc ctacatcgac gccaccatca
    21481 gcgataaccg tcaggaactg atcgccaaaa tgcaggcggg gaaaattcgc ggtctggtgg
    21541 ttattccggt ggatttcgcc gaacagatgg agcgcgccaa cgccatcgca ccgattcagg
    21601 tgattaccga cggcagtgag ccgaataccg ctaactttgt gcaggggtat gtcgaaggga
    21661 tctggcagat ctggcaaatg cagcgagcgg aggataacgg gcagactttt gaaccgctta
    21721 ttgatgtaca aacccgctac tggtttaacc cggcggcgat aagccagcac tttattatcc
    21781 ccggtgcggt gactattatc atgacggtca tcggtgcgat tctcacctcg ctggtggtgg
    21841 cgcgagaatg ggaacgcggc accatggagg ctttgctctc tacggagatt acccgcacgg
    21901 agctgctgct gtgtaagctg atcccttatt actttctcgg gatgctggcg atgttgctgt
    21961 gtatgctggt gtcggtgttt attctcggcg tgccgtatcg cgggtcgctg ctgattctgt
    22021 tttttatctc cagcctgttt ttactcagta ccctggggat ggggctgctg atttccacga
    22081 ttacccgcaa ccagttcaac gccgctcagg tggccctgaa cgcggctttt ctgccgtcga
    22141 ttatgctttc cggctttata tttcagatag acagtatgcc cgcggtgatc cgcgcggtga
    22201 cgtacattat tcccgcacgt tatttcgtca gcactctgca aagcctgttc ctcgccggga
    22261 atattccagt ggtgctgata gtgaacgtgc tgtttttgat cgcttcggcg gtgatgttta
    22321 tcggcctgac gtggctgaaa accaaacgtc ggctggatta gggagaagag catgtttcat
    22381 cgcttatgga cgttaatccg caaagagttg cagtcgctgc tgcgcgaacc gcaaacccgc
    22441 gcaattctga ttttacccgt gctgattcag gtgatcctgt tcccgttcgc cgccacgctg
    22501 gaagtgacca acgccaccat cgccatctac gatgaagata acggcgagca ctcagtagaa
    22561 cttactcaac gttttgcccg cgccagcgcc tttacccatg tgctgctgct gaaaagcccg
    22621 caggagatcc gcccgaccat cgacacacaa aaggcgttac tactggtgcg tttcccggct
    22681 gacttctcgc gcaaactgga taccttccag accgcgccgt tgcagttgat cctcgacggg
    22741 cgtaactcca acagtgcgca aattgccgcc aactacctgc aacagatcgt caaaaattat
    22801 cagcaggagc tgctggaagg aaaaccgaaa cccaacaaca gcgagctggt ggtacgcaac
    22861 tggtataacc cgaatctcga ctacaaatgg tttgtggtgc cgtcgctgat cgccatgatc
    22921 accactatcg gcgtaatgat cgtcacttca ctttccgtcg cccgcgaacg tgaacaaggt
    22981 acgctcgatc agttgctggt ttcgcccctc accacctggc agatattcat cggcaaagcc
    23041 gtaccggcat taattgtcgc caccttccag gccaccattg tgctggcgat tggtatctgg
    23101 gcgtatcaaa tccccttcgc cggatcgctg gcgctgttct actttacgat ggtgatttac
    23161 ggtttatcgc tggtgggatt cggtctgttg atttcatcac tctgttcaac acaacagcag
    23221 gcgtttatcg gcgtgtttgt ctttatgatg cccgccattc tcctttccgg ttacgtttcg
    23281 ccggtggaaa acatgccagt gtggctgcaa aatctgacgt ggattaaccc tattcgccac
    23341 tttacggaca ttaccaagca gatttatttg aaggatgcaa gtctggatat tgtgtggaat
    23401 agtttgtggc cgctactggt gataacggcc acgacagggt cagcggcgta cgcgatgttt
    23461 agacgtaagg tgatgtaact tcttatcttt cgccagcaaa gacaccaccg ccgggccggc
    23521 aaggattgcc agccctgcaa ttgccagcaa aaagttgttt tccagtacgc gggacaacag
    23581 ccacagcaca atcagcgctg caccaaaata ccaggtcgtg gtcagctctt ccagcacgtc
    23641 tgccacctca cgccagcgtt gctctgggtg gcgctgaaac accagcatca tcactaccgt
    23701 gacacaatac agtgcgcata atcctacgcc gacgtgaacc agagttttac tcatccaacc
    23761 caccaccagc agcgccacta ccagtaaatg caacataatc tgccagcaac ttaggccagt
    23821 tgcgacacga acacgttgtt gccacttcat ggcttctctc ctgaagaatt tatctctact
    23881 tattcagagt accgaacttt acggaaaatt ccgtaagacc gcaccttttt gtgacgccga
    23941 ctacactatt tcaatgagat aagaggcaaa gacaggaaaa ttatgcccga tcaaacacaa
    24001 caattttcgt tcaaggtgct caccatcaat attcacaaag gctttaccgc gtttaaccga
    24061 cgcttcattt taccggaact tcgcgacgcc gtgcgtaccg tcagcgccga tattgtttgc
    24121 ctgcaggaag tgatgggcgc gcacgaagtt catccgctgc atgtggaaaa ctggcccgat
    24181 acctcgcact acgagtttct cgccgacacc atgtggagcg atttcgccta cggtcgcaat
    24241 gccgtatacc cggaaggaca tcacggtaac gccgttcttt cacgttatcc cattgaacat
    24301 tatgagaatc gtgatgtttc ggtcgatggt gcggaaaagc gcggcgtgct ctactgccgc
    24361 attgtgccgc cgatgaccgg gaaagcgatt catgtgatgt gcgtacatct gggcctacgt
    24421 gaggcgcacc gtcaggcgca gcttgcgatg ctcgccgaat gggtgaatga gctaccggac
    24481 ggcgaatcgg tattggtagc gggtgatttc aacgactggc ggcaaaaagc taatcatccg
    24541 ttaaaagtgc tggccgggct ggatgagatt tttacccgcg cccacggacg tccggcgcgc
    24601 acgtttccgg tgcaatttcc tctactacga ctggacagga tctacgtcaa aaatgccagc
    24661 gccagcgcgc caaccgcgtt gccgctgcgg acatggcgac acctttctga tcatgcccct
    24721 ttaagtgcgg agattcattt atgaaatgta gctggcgcga aggcaatatg atccagttgc
    24781 tggaaaacgg cgagcaatat tatcccgcgg tgtttaaggc gattggcgag gcgcaagaac
    24841 gcatcattct tgaaacgttt atctggtttg aggatgacgt cggcaaacag ctgcatgcgg
    24901 cgctattggc agcagcgcaa cgcggggtaa aagcggaagt cttgctggat ggctacggtt
    24961 cgccggatct cagcgatgaa tttgtcaatg aactgacggc agctggcgtg gtgttccgct
    25021 actacgatcc ccgccctcgc ctttttggta tgcgcaccaa tgtgtttcgc cggatgcatc
    25081 gcaaaattgt ggtgatcgac gcgcgtatcg cctttattgg cgggctgaat tactccgccg
    25141 agcatatgtc cagctacggc ccggaggcca aacaggatta cgcggtacgc ctcgaagggc
    25201 cgattgtcga agatattctc cagtttgagc tggaaaacct gccaggacaa agcacggcgc
    25261 gacgctggtg gcgacgccat cataaagcgg aagagaaccg ccagccggga gaagcgcagg
    25321 tattgctggt ctggcgcgat aacgaagaac atcgcgatga tatcgaacgt cactatctga
    25381 aaatgctcac tcaggcgcgg cgggaagtga ttatcgccaa cgcctacttc ttccccggct
    25441 atcgtttttt acacgccttg cgtaaagcgg cacgacgcgg ggtgcggatc aaactgatca
    25501 ttcagggcga accggatatg ccgattgtca gagtcggcgc gcgtttgctg tataactatc
    25561 tggttaaagg tggcgttcag gtgtttgagt accgccgccg cccgctccac ggcaaagtgg
    25621 cattgatgga cgatcactgg gcgacagtag ggtccagtaa tctcgatccg ctcagtttgt
    25681 cactgaatct cgaagcaaat gtcatcatcc acgatcgtaa ttttaaccag acgctgcgcg
    25741 ataatctgaa cggcattatc gccgcagatt gtcagcaggt cgatgagacc atgctgccga
    25801 aacgcacctg gtggaacctg actaaaagcg tgctggcgtt ccactttcta cgccacttcc
    25861 cggcgctggt gggctggctt ccggcgcaca cgccacgtct ggcgcaggtt gatccccccg
    25921 cacaaccgac aatggaaacg caggatcggg tagaaactga aaacacgggg gtaaaaccct
    25981 gatgagtaaa tcacacccgc gctggcgctt agcaaagaag atcctcacct ggctgttttt
    26041 tatcgcggtg attgtgttgt tggtggtcta cgccaaaaaa gtggactggg aagaggtctg
    26101 gaaggtcatc cgagactaca atcgcgttgc actgctcagt gcggtcgggc tggtggtcgt
    26161 cagctatctg atttacggtt gctacgacct gctcgcccgt ttctactgcg gtcacaaact
    26221 ggcgaagcgc caggtgatgc tggtgtcgtt tatctgctac gccttcaacc tgacactcag
    26281 tacctgggtc ggcggcattg gtatgcgcta tcgtttgtac tcccgactgg ggttaccggg
    26341 cagcactatt acgcggattt tttcgctcag tattaccacc aactggctag gctatatttt
    26401 gctagcgggg gttatcttta ccgcaggcgt ggtggagttg ccggatcact ggtatgtcga
    26461 tcaaactacg ctgcgtattc tcggcattgg cttactgatg attatcgcgg tttatttgtg
    26521 gttttgcgct ttcgcgaagc accgccatat gaccatcaaa gggcaaaagc tggtgctgcc
    26581 ttcgtggaaa ttcgccctcg cccagatgct gatttccagc gttaactgga tggtaatggg
    26641 agcgattatc tggctgttac ttggtcaaag cgtaagctat ttctttgtac tgggcgtgtt
    26701 gctggttagt agtattgctg gcgtcatcgt gcatattccg gcgggaattg gtgtgctgga
    26761 agcggtgttt atcgcgctac tggctgggga gcatacctcc aaaggcacaa ttatcgccgc
    26821 cctacttgct taccgtgtgc tgtattactt tatcccactg ctgctggcgc tgatctgtta
    26881 cctgttgctg gaaagccagg cgaagaagct acgggcgaaa aatgaagcgg cgatgtgaag
    26941 tatagttaag ttctttagta tgtgcattta cggctaatga aaaaaccgcg tatgcctttg
    27001 ccagacaagc gttatagcta tagcagcaag aatcccaaag aaaccgataa aatagtaata
    27061 gagtgttaat gcacctataa aagcacaacg aaccggactc cataattctt cactatggct
    27121 ttttcctgtc agttcaagga gcttatttcg tccatgcgag ataatgcctg accataagac
    27181 aatcattaag caacttatta cccaaatggg tcgttcactc attaatgtgg cattcacgat
    27241 gattaccagg gccaaccctg tcaacgtcat cataatgata aaacgatgag aaccaggatc
    27301 aaatttaaac aatagacctg ccagcatact gattaaaaac atcgatcccg cagtgctgaa
    27361 cacgacgata atggcagata caggcgctaa actcgcaacg acaatcgcca ccaaagagat
    27421 taaaaacaaa tatattacgc ccgcgatggc ggtatttatc ttgttgatat ctttacttaa
    27481 gccatacata gaaagtaata ataatcctat agcaacatac atttgcggtg aatcatattt
    27541 tgttgccgta atttcgagat acttagcgcc ccaggccagc cctgcggttg ccaccaaacc
    27601 caaagcggta atgagccata tgccgtttaa tatcctggct tcgtgctgaa aatctaattt
    27661 attgctattt tgcgagtatg attccattct tttagttgtc cttagaaaaa acattaatat
    27721 tcaccatcaa taaatttaaa accacgcata caaaatactt ttgcatttat ggatacagaa
    27781 aacgcttccg ctcgtccata aggcatattt tatacctggt acttgttaag tgtcgttttt
    27841 aataagaaaa aaaatcccga cacatggccg ggatattgat gatgaaaaag aattaacggc
    27901 ggttgccgaa gatccgcagc aacatcagga acaggttgat gaagtccaga tacaaggtta
    27961 acgcgccaag aatggaatat ttgcgcaggt tcgacgtgtc gcgagtatca atctgctcac
    28021 ccatattttt cagtttctgg gtgtcatacg ccgtcaatcc gacaaagaca atcacgccga
    28081 tgtaggtgac tgcccacatc aatgcttcgc ttttcagcca gaagttaacc agcgatgcca
    28141 gcacaatgcc gattaacgcc ataaacagca tattgccaaa gccgcttaga tcgcgcttcg
    28201 tggtgtaacc gtacaggctc attgcgccga acatcccggc agtaacgacg aaagtactgg
    28261 cgatagaagc agcggtatag acaatgaaaa tactggaaag cgtaagaccc gtcagcgccg
    28321 aataaagcat aaagagcatc gtcgttacac ctgcgctcag cttttgaatc atcgctgata
    28381 acacaataac caatgctaat tgcgcgatga tcagaccgat taaaaagaca cggttagtaa
    28441 acaacagctc catcacggcc gcggaattag ccgcatacca ggcaacaaat gctgtcagca
    28501 acaagccaac ggtcatccag ccatagactt gcgccatata agtttgcaag ccagcccggg
    28561 gttgtacgat tgaatcagaa cgtgggaatc tgtccatgac tttctcctga agatataagg
    28621 aatatcttaa ggatactgca aaatgatgag gctgtgcatc gacgcagcgt aaacggatgt
    28681 actgagcggt gaaattgccg gacgcagcgg tgccttatcc ggctaacaat aaactaccag
    28741 cgttttgccg cctgctgatc gctctcccga gcttcgaccc agcggtcgcc ttccggcgtg
    28801 gcttcgcgct tccagaacgg tgcgcgggtt ttgagataat ccataataaa ctgcccggct
    28861 tcaaacgcac tgctgcgatg cgcactggta acaccgacaa aaacaatttc atcgcccggc
    28921 cataattcac caatgcggtg aattaccgtg acgcgcccca gcggccagcg gttacgcgct
    28981 tcatcaacga tttctgccag tgctttttct gtcattcccg gatagtgttc gagggttaat
    29041 gctttgacgc tgtcgcccag gttatggttg cgcaccttac cggtaaaggt gactaccgca
    29101 ccgtcttcgt cacgctcagc cagccacggg tactcttctc ctacgctgaa cggctgcggg
    29161 ccgacaacaa ttttggtttc tgccatctta acctccggtt accggcggga agaaagccac
    29221 ttcgtcgccg tcagtcagcg aatggtcaaa actcaccagc gtctggttga cagcagccag
    29281 caatttgcca tcttccagcg ccagagccca gcgatcgctc tgcgcagcca gatgctggcg
    29341 taacgcttca acggttggga aatccgcagc cacttcggtt gcatcagttc ccaccaactc
    29401 gcgcacctgg gcgaaaaaaa gaacgttaat catccgcttc caccttaaag tcacccgact
    29461 tgccgccgct ctttgccagc aaacgtaccg gaccaatcac catatctttt tgcaccgctt
    29521 tgcacatgtc ataaatggtc agcgccgcca cggaggccgc ggttaacgct tccatttcga
    29581 caccggtttt cccggtcagg cggcataagg tttctatacg tacccgattg tgctccggct
    29641 cggcctgtaa attgacttca actttgctga gcatcagcgg gtgacacagc gggatcagat
    29701 cccaggtgcg ttttgccgcc tgaataccgg cgatacgcgc agtggcaaat acgtcgcctt
    29761 tgtggtggcg accatcaata atcatcgcca gcgtctcgct gcgcatggtg acaaaggctt
    29821 cggcgcgcgc ttcacgcacg gtttccgctt tggcggagac atcgaccatg tgcgcttcgc
    29881 cagcggcgtt gatatgggtc agttgcgaca tacttatttc ttcaaatgtg gatggaaatt
    29941 acacggacgc gtacgggcat ccagctgcgg cgcaatgata ttttcccatg cggtacggca
    30001 cgctttggtc gaacccggca tggcgaaaat cagcgttttg ttagcgacgc ccgctaccgc
    30061 acgagattgc aacgtggaag tgccaatctc ttcgaacgac aacatccgga acacttcacc
    30121 aaaaccttca acttcacggt cgaacaacgg cagcagcgct tcgggagcct gatctccttc
    30181 cgtcaggcca gtaccacccg taatcaacac cacttgtaca tcgtcgctgg cgatccacgc
    30241 agagacctga gcgcgaatag cgtagcggtt ttctttcaca atggctttat cgacaacgtg
    30301 atggcccgct tcttgcgccg agtcgcgaag atagtgaccg gaggtatcgt cttcttcacc
    30361 gcgacgatta gaaaccgtaa gaatagcaat acgggtcggg ataaattcag tgcttacctg
    30421 actcatctga tctctccttt tgacgtttta gccaccaatg tacgataagt tttgcgtaat
    30481 accggtgtta ttttgatgca ggaaatgggt ctgttttttc tcccgcagcg ccgctgaaat
    30541 acgcgcttcc agcgcctgtt gctgggtatc gttttccagc agatcgcgca ggttaacgcc
    30601 gccttcacca aacaggcaga gatggagttt accaatggag gaaacgcgca ggcggttgca
    30661 agtggcgcag aaatcttttt catacggcat gataaggcca atctctccgg cgtaatccgg
    30721 atgacaaaag acttgcgcgg gaccgtcgct gcgttgacgt aattggtgga tccagccgcg
    30781 acgcagtaac tcgtcacgca gaacctgacc agagatgtga tgcttacgga agagctcgct
    30841 gccctcgccc gtttccatca gttcgatgaa acgcagctgg atgggacgat gctggatcca
    30901 gttcagaaag gtgtcgagct ggtgatgatt cacatcacgc atcagcacgg tattgacttt
    30961 gaccttctca aaaccggcct caaatgcagc atcgattcct gccatgacct gattgaattt
    31021 atcctgcccg gtaatggcat gaaactggcg agcgtcaagg ctatcaacgc tgacgttgat
    31081 gccagtaagt ccagcatcgc gccagttcgc cacatcgcgc tccagacggt aaccattggt
    31141 ggtgaccgct atctggcgaa tagcgtcgtt ttctcgcaca gcggcgatga tatcggtaaa
    31201 gtcgcggcgt aaagatggct caccacctgt cagacggact ttttcggtgc ccagactggc
    31261 gaaggcgcgc gtaacccggc gaatttcatc gacggtcagg aagcctttat tggtgacgcc
    31321 gctcggcttg taaccatccg gcaggcagta ggtgcaacga aagttacaca catcagtaat
    31381 cgacaggcgc aagtagtaaa acttacgcgc aaatgcatca gtcagttgtg aagccatgta
    31441 cacctttcca gatacgggag gcgaagtcat ttcttccttc gccctggtgg caatctttac
    31501 gagcattgtc acggccaaag caccgtatca gttgacccag gtgcagaggc tagagtgttt
    31561 agtggttaag ccgatactag cgtgtaaatg agatttttac catccacact ttcgctatgt
    31621 aatcatgtat atagcgtcat gatcgtgcta tttcgctaca gaataaggga aaatggcggt
    31681 tttactttga tatacatcat cagggaggca tagcgtgcat cgtcctttag aggtagaaga
    31741 aaatagtttg tatcctcaat attggcaggt taattgctgt ttcctcgcaa tttgcgctac
    31801 tgtagcgcgg ataatctgac tccaggaaac tatatgcgca atcgtacgct ggctgatctt
    31861 gatcgtgtcg ttgctctcgg cggagggcat ggactgggac gcgttctctc atcactttcg
    31921 tctttgggtt ctcgtttaac gggtatcgtc accaccaccg ataacggtgg ctcgacgggg
    31981 cgtattcgcc gctcagaagg cggcattgcc tggggcgata tgcgcaactg cctcaatcag
    32041 ctgataactg aacccagcgt cgcttccgca atgtttgaat atcgtttcgg cggtaatggc
    32101 gaactttccg ggcacaacct tggaaacctg atgttaaagg cactggatca ccttagcgtg
    32161 cgacctcttg aagcgatcaa tctgattcga aatctgctga aagtggacgc acatttgatt
    32221 ccgatgtcgg agcatcctgt tgatctgatg gcgattgacg atcaggggca tgaagtttac
    32281 ggcgaggtca atatcgacca gttaactgcg ccgattcaag agttattgtt aacgccgaat
    32341 gtacccgcaa cgcgtgaggc ggttcacgct atcagtgaag cggatctcat cattattggg
    32401 cctggcagtt tttacaccag cctgatgcca attctgctgc tgaaggaaat cgcacaggca
    32461 ttacgccgca cgccagcgcc gatggtctat atcggcaatc tggggcgtga gttgagttta
    32521 cctgcggcta atctgaagct ggaaagcaag ctggcaatta tggagcagta tgttggtaaa
    32581 aaggtaattg atgcggtcat cgtcgggcca aaagtggatg tctcggcggt gaaagagcgg
    32641 attgtgattc aggaagtgct ggaggccagc gatattccgt atcgtcatga ccgccagttg
    32701 ttgcatagcg cgctggaaaa ggcattacag gctttaggtt aacacgaagc cggaagaata
    32761 ttccggcttc gtcatcttgt cacgacgcgg caataaacag ctcacgcaac tgatgcaact
    32821 ggtcacgaat ttgcgccgct tcttcgaact ccagattctg cgcgtgttgc atcatcaacc
    32881 cttccagttc atggatcttc tgctgtaacg ctttaggcga catatccatc ggcacattat
    32941 ccggctcaac aatcgggcgc gattttcctc tgcccttcgc tttggttttg gcaatgttct
    33001 gccccagcgc caggatatcg accactttct tgttcaagcc ttgcggcgta atgccgtgtt
    33061 cctcgttgta cttctgctgt ttctcgcggc gacgttcggt ttcgccaatc gctttcgcca
    33121 ttgatggggt gatcttatcg ccgtagagaa tcgctttacc gttaacgtta cgtgccgcac
    33181 gaccaatggt ctggatcaac gaacgttcgg aacgcaggaa gccttctttg tcagcgtcga
    33241 ggatcgccac cagcgaaacc tccggcatat ccagaccttc gcgcagtaag ttgatcccta
    33301 ccaatacgtc gaactcaccc agacgcaagt cgcggataat ctccatacgt tcgacggtat
    33361 cgatatctga gtgaagataa cgcacgcgct caccgtgttc ttcgagatat tcagtgagat
    33421 cttccgccat ccgcttggtc agagttgtaa ccagtacgcg ttcgttaatt gccgctcgct
    33481 gacgaatctc cgatagaaga tcatccacct gtgtcgctac cggacgcact tcaataatcg
    33541 ggtcgagtaa gcctgttgga cgcacaacct gatccaccac atcgccaccg gatttttcca
    33601 gctcgtaatt acccggcgtc gccgaaacat agatggtttg cggcgctaat gcttcgaact
    33661 cttcaaattt cagcgggcgg ttatccagcg ctgatggcaa gcggaagccg tactccacca
    33721 gtgtctcttt acgcgcccgg tcaccgcgat acatgccgcc aatttgtgga atggtgacgt
    33781 gagattcatc gaccaccagc agcccatcag caggcaggta atcaaacagc gtcggcggtg
    33841 gctcacccgg tccacgaccg gagaggaagc gcgagtagtt ctcaatcccc gaacagtagc
    33901 ctagctcgtt catcatctcc agatcaaact gggtacgctg ggtcagccgc tgctcttcca
    33961 gcagtttgtt gttttccaat agcactttac gtctggcggc cagctcttct ttgatctcct
    34021 ccatcgcctg gacgatgcgc tcgcgtggcg tgacgtagtg cgttttcggg tagatggtaa
    34081 aacgtggaat agtggaaaca atctgcccgg tcagcgggtc aaataacgac aatcgttcca
    34141 cctcctcgtc aaacagctcc acgcgaagtg caatgtcatc cgattctgcc gggaagatat
    34201 caatcacctc gccacgaaca cggaaagtac cacgctggaa tgcttgatca ttacgggcat
    34261 attgcagctc cgccagtcgg cgcaggatcg cgcgctgatc gataatcata ccgaccgtga
    34321 gatggagcat catcttgaga tataaatcag gatcgcccag accataaatc gcggaaacag
    34381 acgccaccac aaccacatca cgtcgctcca gcatcgcttt ggtggcggat aaacgcatct
    34441 gttcgatatg ttcgtttacc gaggcatctt tctcaataaa ggtgtcggaa ctcggtacat
    34501 aggcctccgg ctgatagtag tcgtagtagg agacgaaata ttccaccgcg ttttctggga
    34561 agaattcttt catttcgcca tacagctggg ccgccagcgt tttgttgggc gcaagtacca
    34621 tggttgggcg ctgaaggtca gcaatgacat tggcaatggt gaaggttttc ccggagccag
    34681 tcacgccaag taacgtctgg tgcgccaggc catcttccag cccctcttcg agacgtcgaa
    34741 tcgcctccgg ctgatcgcca gaaggtttaa aagcggaatt cagtttgaac ggtttactca
    34801 tgagtcgcta cctgaaggag ttgggcgggc aggtatgtaa ttttactcgt cgtacttaat
    34861 tatgccaaca aattatactg gataaaaaaa cagttcatca ccataatatt tctgttacag
    34921 cgtaaactcc gctgtcaatc atgaacaaga tttactctgc ggcgagataa aactccggcc
    34981 ttaccgggtt atccccaaag caacggcttt tttaacattt gtcaagatga ttgatgacag
    35041 ttttatggca agagatgcct gttcagtgac tgccattgca attatctaac ctgttaaaaa
    35101 ataaaagata tttctttgag ctgtctttaa cgccaattcg cgtgcaagcc gcgtattctc
    35161 tcgcttgcct cgtgttttct aactctaata cacatggtta tccacaggaa tagtggataa
    35221 ctgtctgcag cccctatccc ccgccgcctg ggcaattccc acattcaccg catgatgcgg
    35281 aggtaaaatt tttttcgtga tttttttaga atttaattgg ttaaattgca gtcaatcgga
    35341 gacacgatct cgctcgcaat ttaaccaatt acaggatgac tacattaagg caagattgat
    35401 gtactttccg gttgccgcat tttctggatt ttctgcaagc cagggaattt cgcccagcag
    35461 cggcgcggga atcatgcggg tgagcgtggt catatattca gcgtgacgtt ttcccggagg
    35521 cgtgacatcg ttcgccaccc accccgccag agtcagtccg gcgtgtagta ttgcctgcgc
    35581 agtcaacatc gcgtgattaa tacagccgag tttcacgccc acaaccagta tcactggcag
    35641 ttgttcctgt gttacccaat ccgcaaaagt gaaagtgtca gaaagcggcg taaaccagcc
    35701 gccagcacct tccactaaca cccagtcagc ctgttgttca agcgcgcgta atccggcgct
    35761 cattaccaat gcttctatcg gcctgccctc ttgcgcgctg atgatatgcg gcgaggtggg
    35821 ttctgcgaag gtgtaaggat ttaccgttgc gtaatccagc tgcaggctac tgttgcgctg
    35881 taacgccagc gcgtcactat tacgtaagcc tttcggggtc ttttcgctgc cagaggccac
    35941 cggtttgtaa cctgccgtcc ggtagcctgc tgcctttgcg gcttgtaaaa gcgcgcagct
    36001 ggcgacagtt ttccccactt cggtatccgt tccggtgaca aaataacatt tactcacgag
    36061 caatcactcc caaaaaaaga tgatacgtca gaggatatcg cccctgttgt tgcggccagg
    36121 ccagttgcaa tcgctgcaac tgcgaacgcg ttaatattcg cgggtcacgc ccttcatgaa
    36181 gatgcgtggc accgatgcct ttcagcgaac gcatggcact gagcgcatca tcaaaccaca
    36241 gcgtgatggg ctgaatataa tgttgataat gcacggcgtt tagcgactgt tcgatttcat
    36301 ctggcggtaa aaagcgatta gcatgcggac gctcgtccac cgcctgccac gcctgatgca
    36361 gttcgggtaa cgatccctgc accagcgtgg taaacgcgac cacgcctccg gggcgcacca
    36421 cccgatacag ctcgcggagt gccgtggata aattaccgca ccactgcacc gcgagattgc
    36481 tccatgcaag atcgaacgtc gcagtcgcta acggcaggga ttcgatatct cccgccagat
    36541 aatggtctgc ggcatccttc tggcgtgcct gaacaagcat tggcggagag agatctaacg
    36601 cagtcacctg cgcgtgacgc tctcgccagc ggcggctcat ccagcctggt ccacaacccg
    36661 catccagtac gcgggtgtat ttacgctgtg ggagcattgc cagtaaggcg tcagcactct
    36721 ggcgctgtag atcggcgtgt tgctcatagt gtgcggctgc ccgtccaaat gccgcagcaa
    36781 tggcttgttt attaaccgtt gccatgcagc acctccagca gacggtcgat atcctgcatt
    36841 tcatgcgctg cggttagcgt taagcgcagt cgcgcagtac cagcgggtac ggttggcggg
    36901 cgaattgccg tgacccaaca gccttgctgg cgcagttttt ctgccagttg taacgcacgg
    36961 ctgttatcac cgacgatcaa tggctggatg gcgctccatg aatcagcaag cgtaaacggc
    37021 aaatcctgca ctccggcacg aaaacgcgtt atgagtgccg ccagtttttc gcgccgtgca
    37081 tccccctcat cactgcgaat gaccgccagc gacgcacgta atgcctgcgc ctgagcgggc
    37141 ggcatactgg tgctgtagat aagatggcga gcgaattgca gcagataatc cgccaccgta
    37201 ttggagcaaa gcaccgctgc cccgctgacg ccaaatcctt tgccaaacgt caccaccagc
    37261 aattctggtt ttaccttttg cagccagcag ctgccacgcc cctgctcccc gataacgccc
    37321 gtgccgtggg cgtcatcgac catcaaccag ccatcgtgct gttgcgttac ctgctggatt
    37381 tcctccagtg gcgcactatc gccgtccatg ctgaacacgc cttctgtcac taccagttgc
    37441 tgccccggac agggggaagc aagcagtcgc gccagatgag tgacatcgtt atgagcaaaa
    37501 cggcgaagcg gcgacgggct taaacttgca gcctccagca atgaggcatg gctaagccgg
    37561 tcggcaacaa tacggtcctc tttcgccatc atcgcggcaa tgactgcctg attagcagca
    37621 aaaccagaga taaacagcag tgcccgcgaa tagcccagcc actcggccag ttcttcttcc
    37681 agcgcctgat gcgccacgct ataaccgctg acgtgaccgg agccgccgct accgatgcca
    37741 aattgctccg caccctgctt ccaggcacgg ataatttgcg gatgatggct taaacctaaa
    37801 taatcgttac tggaaaagtt cagatactgg cgatcatcca ccaccagcca gcgtccggct
    37861 ccttgcgcca ccggataacg gcgacgcagg gcatcggcag cacgccgcgc atcgagcgcc
    37921 gcgttgattt tctcctgcca gctcatagtg ctgccgcgtt gtaatattcg tcagtgtccg
    37981 gggtcatcag cgcctgctcc agacgctgct gttgttcgtt atcgcccgcc agcacggcag
    38041 tttgctgcgg atttagcccc agtttgcgga acagttgcag gtctttatct tcttccggat
    38101 tcggcgtggt cagcagtttg caaccgtaga aaatcgagtt tgcgccagcc ataaagcaca
    38161 tcgcctgagt ctgttcgttc atctgctcgc gtccggcaga aaggcgtacg taagaggtcg
    38221 gcatcatgat ccgcgcaact gcaatggtgc gaataaaatc aaaggcatcg acatcatcgt
    38281 tatccgccag cggcgtgcct ttcaccttca ccagcatgtt gattggtacg ctttccggcg
    38341 gcgttggcag gtttgccagt tgcagcaata atccagcgcg atcttttact gtttcgccta
    38401 agcccacaat gccgcctgaa cagactttga tcccggcgtc gcgcactttt tccagcgtat
    38461 cgaggcgttc ctgataggta cgcgtggtga tgatattgcc gtaaaactcc ggcgaggtgt
    38521 cgaggttgtg gttgtagtaa tccagcccgg cattcgccag gcgctgcgcc tgagattcac
    38581 tcaacgtgcc cagcgtcata cacgcctcca gccccatcgc ttttaccccc tgcaccattt
    38641 gttccaggta cggcatatcg cgttcgtggg gattcttcca cgccgcgccc atacagaaac
    38701 gcgtcgatcc cgccgctttc gctttgcgcg ccgactccag cacctgttca acttccatca
    38761 accgctcggc ttccagcccg gttttgtagc gcgagctttg cgggcaatat ttgcaatctt
    38821 ccggacaagc tccggtctta atcgacagca aagtgctgac ctgcacctga cgaggatcga
    38881 aatgctgacg atgcacctgc tgcgcttcaa acagcagatc cagcaacggt ttttcaaata
    38941 attctgtgac ttgcgacaat gtccagcgtg ggcggtgagc catggggctt ctccaaaacg
    39001 tgttttttgt tgttaattcg gtgtagactc gtaaacctaa atcttttcaa tttggtttac
    39061 aagtcgatta tgacaacgga cgatcttgcc tttgaccaac gccatatctg gcacccatac
    39121 acatccatga cctccccgct gccggtttat ccggtggcaa gcgccgaagg ttgcgagcta
    39181 attttgtctg acggcagacg cctggttgac ggtatgtcgt cctggtgggc ggcgatccac
    39241 ggttacaatc acccgcagct taatgcggcg atgaagtcgc aaattgatgc tatgtcgcat
    39301 gtgatgtttg gcggtatcac ccatgcgccc gccattgagc tgtgccgcaa attggtggca
    39361 atgacgccgc aaccactgga gtgcgttttt ctcgcggact ccggctccgt agcggtagaa
    39421 gtggcgatga aaatggcgtt gcagtactgg caggccaaag gcgaagcgcg ccagcgtttt
    39481 ctaaccttcc gcaatggtta tcatggcgat acctttggcg cgatgtcggt gtgcgatccg
    39541 gacaactcaa tgcacaacct gtggaaaggc tatctgccag aaaacctgtt tgctcccgcc
    39601 ccgcaaagcc gcatggatgg cgaatgggat gagcgcgata tggtgggctt tgcccgcctg
    39661 atggcggcgc atcgtcatga aatcgcggcg gtgatcattg aaccgattgt ccagggcgca
    39721 ggcgggatgc gcatgtacca tccggaatgg ttaaaacgaa tccgcaaaat gtgcgatcgc
    39781 gaaggtatct tgctgattgc cgacgagatc gccaccggat ttggtcgtac cggcaaactg
    39841 tttgcctgtg aacatgcaga aatcgcgccg gatattttgt gtctcggtaa agccttaacc
    39901 ggcggcacaa tgaccctttc cgccacgctc accacgcgcg aggttgcgga aaccatcagt
    39961 aacggcgaag ccggctgctt tatgcatggg ccaactttta tgggcaatcc gctggcctgc
    40021 gcagcagcaa acgccagcct ggcgattctc gaatctggcg actggcagca tcaggtggcg
    40081 gctattgaag cgcagctacg cgagcaactt gcccccgcct gtgacgccga aatggttgcc
    40141 gatgtgcgcg tactgggggc aatcggcgtg gtcgaaacct ctcgtccggt gaatatggca
    40201 gcgctgcaaa aattctttgt cgaacagggt gtctggatcc ggccttttgg caaactgatt
    40261 tacctgatgc cgccctatat tattctcccg caacagttgc agcgtctgac cgcagcggtt
    40321 aatcgtgcgg tgcaggatga aacatttttt tgccaataac gggaagtcag cgtgagggtt
    40381 tctggctaca ctttctgcaa acaagaaagg agggctcatg aaactcatca gtaatgattt
    40441 acgcgatggc gataagctgc cgcatcgtca tgtctttaac ggcatgggtt acgatggcga
    40501 taatatttca ccgcatctgg cgtgggatga tgttcctgtg ggaacgaaaa gttttgttgt
    40561 cacctgctac gacccggatg cgccaactgg ctccggctgg tggcactggg tagttgttaa
    40621 tttacccgcg gatacccgcg tattaccgca agggtttgga tctggtctgg tagcaatgcc
    40681 agacggcgtt ttgcagacgc gtaccgactt tggtaaaacc ggatacgatg gcgcagcgcc
    40741 gccgaaaggc gaaacccatc gctacatttt taccgttcac gcgctggatg tagaacgtat
    40801 tgatgtcgat gaaggtgcca gcggcgcgat ggtcgggttt aacgttcatt ttcactctct
    40861 ggcgagtgcc tcgattactg cgatgtttag ttaatcactc tgccagatgg cgcaatgcca
    40921 tctggtatca cttaaaggta ttaaaaacaa ctttttgtct ttttaccttc ccgtttcgct
    40981 caagttagta taaaaaagca ggcttcaacg gattcatttt tctatttcat agcccggagc
    41041 aacctgtgaa cacattttca gtttcccgtc tggcgctggc attggctttt ggcgtgacgc
    41101 tgaccgcctg tagctcaaca ccgcccgatc aacgtccttc tgatcaaacc gcgcctggta
    41161 cctcttctcg cccgattctg tcggcaaaag aagcgcagaa tttcgatgct caacactatt
    41221 ttgcatccct gacaccaggt gcggcagcgt ggaatccttc cccgattacc ctgcctgcgc
    41281 aacctgactt tgttgtcggc ccggcgggta ctcaaggtgt aacgcatacc acgattcagg
    41341 cggcggtaga tgcggcaatt atcaagcgta ccaacaagcg ccagtatatt gccgtgatgc
    41401 ctggtgagta tcagggaacg gtgtatgtcc ctgccgctcc gggtggaatt actctgtacg
    41461 gtacaggtga aaaaccgatt gatgtgaaga ttgggctttc ccttgatggt ggcatgagcc
    41521 ctgccgactg gcgtcacgac gtcaacccgc gcggcaaata tatgccaggt aaaccggcgt
    41581 ggtatatgta cgatagctgc cagagtaaac gcagcgacag tatcggtgtt ctctgctctg
    41641 cggtcttctg gtcacaaaac aatggcctgc aactgcaaaa cctgaccatc gaaaacacgc
    41701 tgggcgatag cgtagatgcg ggtaaccatc cggcggtggc actgcgtact gatggcgaca
    41761 aagtgcagat caataacgtc aacattctcg gtcgtcagaa caccttcttt gtcaccaaca
    41821 gcggtgtgca gaaccgtctg gaaacgaatc gccagccgcg tacgctggtg accaacagct
    41881 atattgaagg ggatgtggat atcgtttctg gtcgcggcgc agtggtgttc gataacaccg
    41941 aattccgcgt ggtgaactcc cgtacccagc aagaagcgta tgtgtttgca ccggctacgc
    42001 tgtccaacat ttactacggt ttcctcgccg taaacagccg tttcaatgct tccggtgatg
    42061 gcgtggcgca actgggccgc tcgctggatg ttgatgccaa taccaacggt caggtagtga
    42121 tccgtgatag cgccatcaac gaaggtttta acacagccaa accgtgggct gatgcggtga
    42181 tctctaatcg cccgtttgcg ggtaacaccg gcagcgttga tgataacgac gaagtacaac
    42241 gcaatctgaa tgacactaac tacaaccgca tgtgggaata caataaccgc ggcgtgggta
    42301 gcaaagtggt tgcagaggcg aagaagtagg agctgttaac tatgtgccgg atgcggcgta
    42361 aacgccttat ccggcctaca gttcgatacg atttgtcggt cggataagat gcgccagcat
    42421 cgcatccgac aataagtgcc ggatgcgacc tacattcaca tggcgctttt tacatctgac
    42481 ggtttttatt gaagttaatc aaactacccg ccttgataat ctcgcgttct tcggcagtca
    42541 gactttccat atagagcgtg atttccgtta ccggtgcatc ttcatggatc acataacctt
    42601 taaacgtcgt tccaggatta tccagcgccg ctttaatgcc agggatgtaa atgtaatccc
    42661 ccacttcaaa ggttggcgct tccgccatct gcagcggtaa catcccccag ttgatgacgt
    42721 tagaacgata gcgtttagtc gcgtactcct cggcaatatt cgccagaccg ccaatcacac
    42781 gctgacaact cgccgcctgt tcacgcgcag aaccatcgcc tggtttcacc gcatagacca
    42841 tgctgccaat ttcagtttgc agcggatcaa catgttcctg accagcaatc tgcttaatgc
    42901 gcgcaaacac ctctgccatc tcgctgacat tccccgccag acgctgattt tccagctcag
    42961 cagtcgcttt acttctgcca acataacctg gatcgcggcg tgacagggta aactccgcca
    43021 gaccaatcgg atttgaacga taagaagagg tttcaccgga aggaatcagt tcgtcggtgg
    43081 tggtcacttc gtcgaggatc ttcgagcaca ctttgaggac gatattgtca gtcagcgcac
    43141 ccaattccgg ccagtcttta atgttcggtc cgtaaatcag cggttgctgg gtcgcccctt
    43201 tcacaaagcc ttgatagacg cgatttttat acggcgttac atcgaaggcg tactccggca
    43261 cgttgtccca gcaatcgagt tcgctggcag aggttaaata gccaccgttt gccgcagtcg
    43321 cagcgataga acgagcgtcc atcaacgcca ccgctgacat ctgcccatta gctggcttag
    43381 agccttcgcg gttcgggaag ttacgcgtgg tgtggcgaat actcaaaccg ttgttgattg
    43441 gcgtatcgcc cgcgccaaag catgggccgc agaacgcggt tctgatgatt gcgcctgcgc
    43501 caatcaaatc tgcgaccaca ccttttttgg cgagatccat aaacaccggc tgtgatgacg
    43561 ggtaaaccgc taacgagaag gtgtcattgc cgcaggattg accgcgcagc gcattcgccg
    43621 ccgcgatgac gttttcatag ttaccgccag aacagcccgc gataatcccc tgctgcactt
    43681 tcaggcgacc attttccact ttatccagca gcgagagttt ggctttaccg tgcgccacgc
    43741 gttcggactc aatttcaatc tcacgcagaa tgtcggtcag gttctggttc agcgtgtcga
    43801 tttcatacac gttgctcggg tggaatggca gcgcaatcat tggtttgatg gcgcttaaat
    43861 caacgctgat gcagccatcg tagtacgcca ttggttgagg gttaagttgg cagtaatcct
    43921 ggccgcgacc gtgcagcgcc agccagttat ggacttcttc atcggtttgc cagacagaac
    43981 ttaaacaggt cgtttcagtg gtcatcacgt caacgctgtt acggaaatcg gtagagagcg
    44041 ctgcaacacc gggacctacg aactccatca ctttgttttt gacgtaaccg tttttgaaca
    44101 cggcaccgat gatagccagc gccacatcct gcggccccac atacggcgca ggttttccgg
    44161 tcagatgcac cgcaaccacg cccggatagt cgatatccca ggtgtcatta agcagttgtt
    44221 ttaccagctc gccgccgccc tcaccgactg ccattgtccc taatgcgccg taacgggtgt
    44281 ggctgtctga tccgaggatc attttgccgc cgccagccat catttcacgc atatattgat
    44341 ggatgaccgc aatatgcggt ggcacaaaaa taccgccata acgctgggcc gccgataaac
    44401 caaaaacatg gtcatcaccg ttaatggtgc cgccgacggc gcagagtgag ttatggcagt
    44461 tggtcagcac atacggcagc gggaaacgtt ccataccgga cgctttagcg gtctgtacaa
    44521 taccaacaaa ggtaatatcg tgagaggcta atgaatcaaa cttaatttta agtttatcca
    44581 tatttccgga cgtattatga gaagagagaa tagaccaggc aatagtgcct tttttggctt
    44641 cttctttttt aatttcgccg gtgaaatgtt cttcggcaat tatttcgttt ttactggcga
    44701 gaaacacgcc tttttcagat aacttgatca tcaaatgctc cagatatctg cgcaccggaa
    44761 agtgcgcaga aagataataa atggatatac tctaaataat tcgagttgca ggaaggcgac
    44821 aagcgagtga atccccagtc acttacataa gtaagtgact ggggtgagcg aacgcagacg
    44881 cagcacatgc aacttgaagt atgacgagta tatttacagc atgttccagc cgatcagcat
    44941 attccaccac cacacgccga gggtgatatg caccaggaag gttaatatcg tcagtaccgc
    45001 accgaccaac caccaggatt taatatcgtt gtaacccacg ccaaagatga ccggacctgc
    45061 cgcgccgcca tagtgagtaa ccatgccgcc ataggagtta gagaacaaca gtgccagcgc
    45121 ggttaacatt aacggtgcgc cggagacatt cgccagcatg gcaaataccg gtaacatggc
    45181 aacgatatag gcactaccag aagcgaagaa ataacgcacg atgatgctga ggaaaataat
    45241 aacgaagaaa gcaacgttcc cgtgaccatc aaatgccagg ttatttttaa agacttcagc
    45301 taaccattcg aagaatttaa ctttcgataa taaggagctt aagccgataa taccgccgta
    45361 ccagattaag gtattccagc cgcctttatt tttaaccacg tcttcccagg taacgatacc
    45421 cagcagcagc atagttgcca taacaacgat tgccacggtg gattcatcaa cccccagaga
    45481 cttactgaaa atccagccca gcagcgccag cacaaagaca ccgagcagca ttttttcgcg
    45541 gattttcatc ggtcccagtt cggcaaggcc cgctttagcg atggttttgt tatccacctt
    45601 cttaatttct ggtggataca tggtgtaaat caccagcggg gtgaccagca gcataatgat
    45661 gcccggcaat ccggcggcta acgcccatcc accccagcta atttgcaggt gcagaatgtc
    45721 gttgatcatt ttcagcgcca gaatgttccc cgccattgcg gtaaagaaca tatagctggt
    45781 ggttttggtg accatgtaaa tggacatcat caggtaatgt ccaacacgac gcggactttt
    45841 ttccggttct gatcccaaag ccactgccac gctgttgatg atcggtaaca cgatgccgcc
    45901 cgcacgcgcg gtgttagacg gtgttgccgg agccagtacc agatcgagaa ataccgtaac
    45961 gtaacccaga cccagggtag tgctaccaat tttaccaatc agcagatagg caatacgttt
    46021 acctaagcct gtggttacaa acgcggcgct taaagtaaac gcagaaaaca ccagccaggt
    46081 ggtaccggaa gagtaaccgc ttaatacagc ggtggtttta aattccccac ctgataagtt
    46141 acccaccact accatcgatg cggcgacggc aattaacagt acgacaggtt ccgggaaagg
    46201 cttgataacc agccccacaa tggccgccag gtaaatacca aaaagcaccc acgccagttc
    46261 gcttaatcct gccggagctg gcataaagcc aataatacat gggattgcta atatcagaat
    46321 tagcttccat aacgatttct tattcattta atatcctgtt ctttctgtat gactcataca
    46381 gaattccttt attgattgtc attatcacga caacaaaatt tttcagggaa gataaacttc
    46441 accggaaaat atttttctgg tcgtccgaat aacagatgcg cgtaacgtcg tggcatcctg
    46501 accttcatta cttaaatgaa cgtcgagcgc accactgggg tgttcaatat tgatattgcc
    46561 gtatcctaca gaagggacga tttgtcgggt gacggtacct tccaatgcac aactactgga
    46621 aatagcaata gcaccggtta tcgccaaagc gcgatggcaa gaatgcggca taaaataacg
    46681 cacattaatt gccccgcctt tctgcgctgg agaaataagt acaggtttag ggataaccat
    46741 attactgaca tcgcctaaac ccattgcttt acccgcttgt agacggatag attcaatgcg
    46801 ggctaataat gctttgtcgg catccagctc tgccggtaat tcatagcctg tttttcccag
    46861 atattcagca ggaataatga caactggcat cgccatatcg atacaggtca ccgggacatc
    46921 gtcaaaataa tcaatctgat tatccgtcgg gaagactttt ccggttttcg ttccggcggc
    46981 attcaggaaa gtgagcgcaa ccggtgcggc agtacccggt acgccgtcaa ttctggcgct
    47041 accctcgtac tcgacaacac catttggcgt ttgcacatca gcttcgatga acgtacccgt
    47101 attgacgttg cggatacgta cgcgggtaac tggcgaagtc gctgcaatca aaccattttc
    47161 aatggcgaat gccccaacgc cagacagcat attgccgcag ttaggcgtgg tatcgacacg
    47221 ttgctcatgg acgataactt gagcaaacag ataatcgaca tctgcacgcg gatcgctgga
    47281 gcggctaata atggcgactt tactggtcag cggattaccg ccgccaatac cgtcaatttc
    47341 cagatcgtta ccggaaccca taattgccat caatatttta tcgcgctgcg tttgatcttc
    47401 gggtagatgt tccgctaaca ggaacgcgcc cctcgaggtt ccacctcgca tcatcacgca
    47461 gggtattttt ttcataataa tgccctcaat ttaaaaacat aactta
//
LOCUS       DAHHTG010000022        45061 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.022, whole genome shotgun sequence.
ACCESSION   DAHHTG010000022 DAHHTG010000000
VERSION     DAHHTG010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 45061)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45061)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45061
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.022"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            200..577
                     /locus_tag="NBI74_001231"
     CDS             200..577
                     /locus_tag="NBI74_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000866321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HCD1931595.1"
                     /translation="MKVLLSIKPEFAEKILNGTKRFEFRKGIFKNPQISTVVIYATMP
                     LGKVVGQFRIESILSDEPESLWKKTEKHAGISKQFYDSYYSGREKAYAIKIGEVERYK
                     EPIPISALGSNIKPPQSYLYLPA"
     gene            1053..1259
                     /locus_tag="NBI74_001232"
     CDS             1053..1259
                     /locus_tag="NBI74_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001524508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibitor protein"
                     /protein_id="HCD1931596.1"
                     /translation="MVHQHYGTQTVNRGAVMPGMLVKHKDGTWTASANLRGRLYLHRG
                     IERTYTRDLLVEVFLDGRGNGLNH"
     gene            1335..1631
                     /locus_tag="NBI74_001233"
     CDS             1335..1631
                     /locus_tag="NBI74_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host-nuclease inhibitor protein Gam"
                     /protein_id="HCD1931597.1"
                     /translation="MNAYYIQDRLEAQSWARHYQQIAREEKEAELADDMEKGLPQHLF
                     ESLCIDHLQRCGANKKAITRAFDDDVEFQERMAEHIRYMVETIAHHHVDIDSEV"
     gene            1637..2422
                     /gene="bet"
                     /locus_tag="NBI74_001234"
     CDS             1637..2422
                     /gene="bet"
                     /locus_tag="NBI74_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage recombination protein Bet"
                     /protein_id="HCD1931598.1"
                     /translation="MSTALATLAGKLAERVGMDSVDPQELITTLRQTAFKGDASDAQF
                     IALLIVANQYGLNPWTKEIYAFPDKQNGIVPVVGVDGWSRIINENQQFDGMDFEQDNE
                     SCTCRIYRKDRNHPICVTEWMDECRREPFKTREGREITGPWQSHPKRMLRHKAMIQCA
                     RLAFGFAGIYDKDEAERIVENTAYTAERQPERDITPVNDETMQEINTLLIALDKTWDD
                     DLLPLCSQIFRRDIRASSELTQAEAVKALGFLKQKATEQKVAA"
     gene            2419..3099
                     /locus_tag="NBI74_001235"
     CDS             2419..3099
                     /locus_tag="NBI74_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqaJ viral recombinase family protein"
                     /protein_id="HCD1931599.1"
                     /translation="MTPDIILQRTGIDVRAVEQGDDAWHKLRLGVITASEVHNVIAKP
                     RSGKKWPDMKMSYFHTLLAEVCTGVAPEVNAKALAWGKQYENDARTLFKFTSGVNVTE
                     SPIIYRDESMRTACSPDGLCSDGNGLELKCPFTSRDFMKFRLGGFEAIKSAYMAQVQY
                     SMWVTRKDAWYFANYDPRMKREGLHYVVVERDEKYMASFDEMVPEFIEKMDEALAEIG
                     FVFGEQWR"
     gene            3096..3278
                     /locus_tag="NBI74_001236"
     CDS             3096..3278
                     /locus_tag="NBI74_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1317 domain-containing protein"
                     /protein_id="HCD1931600.1"
                     /translation="MTHPHDNIRVGAITFVYSVTKRGWVFPGLSVIQNPLKAQRLAEE
                     INNKRGAVCTKHLPLS"
     gene            3251..3442
                     /locus_tag="NBI74_001237"
     CDS             3251..3442
                     /locus_tag="NBI74_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1382 family protein"
                     /protein_id="HCD1931601.1"
                     /translation="MHKASPVELRTSIEMAHSLAQIGVRFVPIPVETDEEFHTLATSL
                     SQKLEMMVAKAEADERDQV"
     gene            3519..3734
                     /locus_tag="NBI74_001238"
     CDS             3519..3734
                     /locus_tag="NBI74_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HCD1931602.1"
                     /translation="MTPQQENALRSIARQANYEIKKARQQFPDKNVDDICRSVLKKHR
                     ETVTLMGFTPTHLSLAIGMLNGVFKER"
     gene            3833..4051
                     /locus_tag="NBI74_001239"
     CDS             3833..4051
                     /locus_tag="NBI74_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016246897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HCD1931603.1"
                     /translation="MADIIDSASEIEELQRNTAIKMRRLNHQAISATHCCECGDPIDE
                     RRRLAVQGCRTCASCQEEIELKNKQWGL"
     gene            4099..4338
                     /locus_tag="NBI74_001240"
     CDS             4099..4338
                     /locus_tag="NBI74_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016248605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HCD1931604.1"
                     /translation="MFRIIFPNTWYVDHHGTPCKILRSTHNKVHYIRKGRTCIASMFR
                     FNHDFEPVNKADADRIAEEIETAEHIKKLRAIRRK"
     gene            4478..4714
                     /locus_tag="NBI74_001241"
     CDS             4478..4714
                     /locus_tag="NBI74_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HCD1931605.1"
                     /translation="MSRLITLQDWAKEEFGDLAPSERVLKKYAQGKMMAPPAIKVGRY
                     WMIDRNSRFVGTLAEPQLPINANPKLQRIIADGC"
     gene            4704..5846
                     /locus_tag="NBI74_001242"
     CDS             4704..5846
                     /locus_tag="NBI74_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase Arm DNA-binding
                     domain-containing protein"
                     /protein_id="HCD1931606.1"
                     /translation="MAARPRSHKISIPNLYCKLDKRTGKVYWQYKHPLSGRFHSLGTD
                     ENEAKQVATEANTIIAEQRTRQILSVNERLERMKGRRSDITVTEWLDKYISIQEDRLQ
                     HNELRPNSYRQKGKPIRLFREHCGMQHLKDITALDIAEIIDAVKAEGHNRMAQVVRMV
                     LIDVFKEAQHAGHVPPGFNPAQATKQPRNRVNRQRLSLPEWQAIFDSVSRRQPYLKCG
                     MLLALVTGQRLGDICNLKFSDIWDDMLHITQEKTGSKLAIPLNLKCDALNITLREVIS
                     QCRDAVVSKYLVHYRHTTSQANRGDQVSANTLTTAFKKAREKCGIKWEQGTAPTFHEQ
                     RSLSERLYREQGLDTQKLLGHKSRKMTDRYNDDRGKDWIIVDIKTA"
     gene            complement(5960..7210)
                     /gene="icd"
                     /locus_tag="NBI74_001243"
     CDS             complement(5960..7210)
                     /gene="icd"
                     /locus_tag="NBI74_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HCD1931607.1"
                     /translation="MESKVVVPAQGKKITLQNGKLNVPENPIIPYIEGDGIGVDVTPA
                     MLKVVDAAVEKAYKGERKISWMEIYTGEKSTQVYGQDVWLPAETLDLIREYRVAIKGP
                     LTTPVGGGIRSLNVALRQELDLYICLRPVRYYQGTPSPVKHPELTDMVIFRENSEDIY
                     AGIEWKADSADAEKVIKFLREEMGVKKIRFPEHCGIGIKPCSEEGTKRLVRAAIEYAI
                     ANDRDSVTLVHKGNIMKFTEGAFKDWGYQLAREEFGGELIDGGPWLKVKNPNTGKEIV
                     IKDVIADAFLQQILLRPAEYDVIACMNLNGDYISDALAAQVGGIGIAPGANIGDECAL
                     FEATHGTAPKYAGQDKVNPGSIILSAEMMLRHMGWTEAADLIVKGMEGAINAKTVTYD
                     FERLMEGAKLLKCSEFGDAIIKNM"
     gene            7382..8035
                     /gene="rluE"
                     /locus_tag="NBI74_001244"
     CDS             7382..8035
                     /gene="rluE"
                     /locus_tag="NBI74_001244"
                     /EC_number="5.4.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415653.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2457) synthase RluE"
                     /protein_id="HCD1931608.1"
                     /translation="MRQFIISENTMQKTSFRNHQVKRFSSQRSTRRKPDNQPTRVILF
                     NKPYDVLPQFTDEAGRKTLKEFIPVQGVYAAGRLDRDSEGLLVLTNNGALQARLTQPG
                     KRTGKIYYVQVEGIPTQDALEALRNGVTLNDGPTLPAGAELVEEPAWLWPRNPPIRER
                     KSIPTSWLKITLYEGRNRQVRRMTAHVGFPTLRLIRYAMGDYSLDNLANGEWRDATD"
     gene            8045..8506
                     /gene="nudJ"
                     /locus_tag="NBI74_001245"
     CDS             8045..8506
                     /gene="nudJ"
                     /locus_tag="NBI74_001245"
                     /EC_number="3.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase NudJ"
                     /protein_id="HCD1931609.1"
                     /translation="MFKPHVTVACVVHAEGKFLVVEETINGKALWNQPAGHLEADETL
                     VEAAARELWEETGISAQPQHFIRMHQWIAPDKTPFLRFLFAIELEQICPTQPHDSDID
                     CCRWVSAEEILQASNLRSPLVAESIRCYQSGQRYPLEMIGDFNWPFTKGVI"
     gene            8560..9666
                     /gene="mnmA"
                     /locus_tag="NBI74_001246"
     CDS             8560..9666
                     /gene="mnmA"
                     /locus_tag="NBI74_001246"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309632.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HCD1931610.1"
                     /translation="MSETAKKVIVGMSGGVDSSVSAWLLQQQGYQVEGLFMKNWEEDD
                     GEEYCTAAADLADAQAVCDKLGIELHTVNFAAEYWDNVFELFLAEYKAGRTPNPDILC
                     NKEIKFKAFLEFAAEDLGADYIATGHYVRRADVDGKSRLLRGLDSNKDQSYFLYTLSH
                     EQIAQSLFPVGELEKPQVRKIAEDLGLVTAKKKDSTGICFIGERKFREFLGRYLPAQP
                     GKIITVDGDEIGEHQGLMYHTLGQRKGLGIGGTKDGTEEPWYVVDKDVENNILIVAQG
                     HEHPRLMSVGLIAQQLHWVDREPFTGTMRCTVKTRYRQTDIPCTVKALDDDRIEVIFD
                     EPVAAVTPGQSAVFYNGEVCLGGGIIEQRLPLPV"
     gene            9702..10343
                     /gene="hflD"
                     /locus_tag="NBI74_001247"
     CDS             9702..10343
                     /gene="hflD"
                     /locus_tag="NBI74_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high frequency lysogenization protein HflD"
                     /protein_id="HCD1931611.1"
                     /translation="MAKNYYDITLALAGICQSARLVQQLAHQGHCDGDALHVSLNSII
                     DMNPSSTLAVFGGSEANLRVGLETLLGVLNASSRQGLNAELTRYTLSLMVLERKLSSA
                     KGALDTLGNRINGLQRQLEHFDLQSETLMSAMAAIYVDVISPLGPRIQVTGSPAVLQS
                     PQVQAKVRATLLAGIRAAVLWHQVGGGRLQLMFSRNRLTTQAKQILAHLTPEL"
     gene            10347..11717
                     /gene="purB"
                     /locus_tag="NBI74_001248"
     CDS             10347..11717
                     /gene="purB"
                     /locus_tag="NBI74_001248"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002950897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HCD1931612.1"
                     /translation="MELSSLTAVSPVDGRYGDKVSALRGIFSEYGLLKFRVQVEVRWL
                     QKLAAHAAIKEVPAFAADAIGYLDAIVASFSEEDAARIKTIERTTNHDVKAVEYFLKE
                     KVADIPELHAVSEFIHFACTSEDINNLSHALMLKTARDEVILPYWRQLIDGIKDLAVQ
                     YRDIPLLSRTHGQPATPSTIGKEMANVAYRMERQYRQLNQVEILGKINGAVGNYNAHI
                     AAYPEVDWHQFSEEFVTSLGIQWNPYTTQIEPHDYIAELFDCVARFNTILIDFDRDVW
                     GYIALNHFKQKTIAGEIGSSTMPHKVNPIDFENSEGNLGLSNAVLQHLASKLPVSRWQ
                     RDLTDSTVLRNLGVGIGYALIAYQSTLKGVSKLEVNHDHLLEELDHNWEVLAEPIQTV
                     MRRYGIEKPYEKLKELTRGKRVDAEGMKQFIDGLALPEEEKARLKAMTPANYIGRAIT
                     MVDELK"
     gene            11886..12557
                     /gene="phoP"
                     /locus_tag="NBI74_001249"
     CDS             11886..12557
                     /gene="phoP"
                     /locus_tag="NBI74_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator PhoP"
                     /protein_id="HCD1931613.1"
                     /translation="MRVLVVEDNALLRHHLKVQIQDAGHQVDDAEDAKEADYYLNEHL
                     PDIAIVDLGLPDEDGLSLIRRWRSNDVSLPILVLTARESWQDKVEVLSAGADDYVTKP
                     FHIEEVMARMQALMRRNSGLASQVISLPPFQVDLSRRELSINDEVIKLTAFEYTIMET
                     LIRNNGKVVSKDSLMLQLYPDAELRESHTIDVLMGRLRKKIQAQYPQEVITTVRGQGY
                     LFELR"
     gene            12557..14017
                     /gene="phoQ"
                     /locus_tag="NBI74_001250"
     CDS             12557..14017
                     /gene="phoQ"
                     /locus_tag="NBI74_001250"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PhoQ"
                     /protein_id="HCD1931614.1"
                     /translation="MKKLLRLFFPLSLRVRFLLATAAVVLVLSLAYGMVALIGYSVSF
                     DKTTFRLLRGESNLFYTLAKWENNKLHVELPENIDKQSPTMTLIYDENGQLLWAQRDV
                     PWLMKMIQPDWLKSNGFHEIEADVNDTSLLLSGDHSIQQQLQEVREDDDDAEMTHSVA
                     VNVYPATSRMPKLTIVVVDTIPVELKSSYMVWSWFIYVLSANLLLVIPLLWVAAWWSL
                     RPIEALAKEVRELEEHNRELLNPATTRELTSLVRNLNRLLKSERERYDKYRTTLTDLT
                     HSLKTPLAVLQSTLRSLRSEKMSVSDAEPVMLEQISRISQQIGYYLHRASMRGGTLLS
                     RELHPVAPLLDNLTSALNKVYQRKGVNISLDISPEISFVGEQNDFVEVMGNVLDNACK
                     YCLEFVEISARQTDEHLYIVVEDDGPGIPLSKREVIFDRGQRVDTLRPGQGVGLAVAR
                     EITEQYEGKIVAGESMLGGARMEVIFGRQHSAPKDE"
     gene            14093..15214
                     /gene="roxA"
                     /locus_tag="NBI74_001251"
                     /gene_synonym="ycfD"
     CDS             14093..15214
                     /gene="roxA"
                     /locus_tag="NBI74_001251"
                     /gene_synonym="ycfD"
                     /EC_number="1.14.11.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[50S ribosomal protein L16]-arginine
                     3-hydroxylase"
                     /protein_id="HCD1931615.1"
                     /translation="MEYQLTLNWPDFLERHWQKRPVVLKRGFNNFIDPISPDELAGLA
                     MESEVDSRLVSHQDGKWQVSHGPFESYDHLGETNWSLLVQAVNHWHEPTAALMRPFRE
                     LPDWRIDDLMISFSVPGGGVGPHLDQYDVFIIQGTGRRRWRVGEKLQMKQHCPHPDLL
                     QVDPFEAIIDEELEPGDILYIPPGFPHEGYALENAMNYSVGFRAPNTRELISGFADYV
                     LQRELGGNYYSDPDVPPRAHPADVLPQEMDKLREMMLELINQPEHFKQWFGEFISQSR
                     HELDIAPPEPPYQPDEIYDALKQGDVLVRLGGLRVLRIGDDVYANGEKIDSPHRPALD
                     ALASNIALTAENFGDALEDPSFLAMLAALVNSGYWFFEG"
     gene            complement(15263..16489)
                     /gene="pepT"
                     /locus_tag="NBI74_001252"
     CDS             complement(15263..16489)
                     /gene="pepT"
                     /locus_tag="NBI74_001252"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HCD1931616.1"
                     /translation="MDKLLERFLNYVSLDTQSKAGMRQVPSTEGQWKLLHLLKEQLEE
                     MGLINVTLSEKGTLMATLPANVPGDIPAIGFISHVDTSPDCSGKNVNPQIVENYRGGD
                     IALGIGDEVLSPVMFPVLHQLLGQTLITTDGKTLLGADDKAGIAEIMTALAVLQQKNI
                     PHGDIRVAFTPDEEVGKGAKHFDVDAFDARWAYTVDGGGVGELEFENFNAASVNIKIV
                     GNNVHPGTAKGVMVNALSLAARIHAEVPADESPEMTEGYEGFYHLASMKGTVERADMH
                     YIIRDFDRKQFEARKRKMMEIAKKVGKGLHPDCYIELVIEDSYYNMREKVVEHPHILD
                     IAQQAMRDCDIEPELKPIRGGTDGAQLSFMGLPCPNLFTGGYNYHGKHEFVTLEGMEK
                     AVQVIVRIAELTAQRK"
     gene            16739..17875
                     /gene="potA"
                     /locus_tag="NBI74_001253"
     CDS             16739..17875
                     /gene="potA"
                     /locus_tag="NBI74_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     ATP-binding protein PotA"
                     /protein_id="HCD1931617.1"
                     /translation="MGQSKKLNKQPSSLSPLVQLAGIRKCFDGKEVIPQLDLTINNGE
                     FLTLLGPSGCGKTTVLRLIAGLETVDSGRIMLDNEDITHVPAENRYVNTVFQSYALFP
                     HMTVFENVAFGLRMQKTPAAEITPRVMEALRMVQLETFAQRKPHQLSGGQQQRVAIAR
                     AVVNKPRLLLLDESLSALDYKLRKQMQNELKALQRKLGITFVFVTHDQEEALTMSDRI
                     VVMRDGRIEQDGTPREIYEEPKNLFVAGFIGEINMFNATVIERLDEQRVRANVEGREC
                     NIYVNFAVEPGQKLHVLLRPEDLRVEEINDDNHAEGLIGYVRERNYKGMTLESVVELE
                     NGKMVMVSEFFNEDDPDFDHSLDQKMAINWVESWEVVLADEEHK"
     gene            17859..18722
                     /gene="potB"
                     /locus_tag="NBI74_001254"
     CDS             17859..18722
                     /gene="potB"
                     /locus_tag="NBI74_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309597.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotB"
                     /protein_id="HCD1931618.1"
                     /translation="MKNTSKFQNVVIVTIVGWLVLFVFLPNLMIIGTSFLTRDDASFV
                     KMVFTLDNYTRLLDPLYFEVLLHSLNMALIATLACLVLGYPFAWFLAKLPHKVRPLLL
                     FLLIVPFWTNSLIRIYGLKIFLSTKGYLNEFLLWLGVIDTPIRIMFTPSAVIIGLVYI
                     LLPFMVMPLYSSIEKLDKPLLEAARDLGASKLQTFIRIIIPLTMPGIIAGCLLVMLPA
                     MGLFYVSDLMGGAKNLLIGNVIKVQFLNIRDWPFGAATSITLTIVMGLMLLVYWRASR
                     LLNKKVSELDD"
     gene            complement(18954..19220)
                     /locus_tag="NBI74_001255"
     CDS             complement(18954..19220)
                     /locus_tag="NBI74_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001387760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HCD1931619.1"
                     /translation="MLPQHSDIEIAWYASIQQEPNDWKTVTTQFHIQEFSEYIAPLQD
                     AVDLEIATAEERSLLEAWNKYRVLLNRVDTSTAPDIEWPTSPAE"
     gene            19277..19945
                     /locus_tag="NBI74_001256"
     CDS             19277..19945
                     /locus_tag="NBI74_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCD1931620.1"
                     /translation="MVKSMKKNTDDGAKIYTPLTLKLYDWWVLGVSNRLAWGCPTKEH
                     LLPHFLEHVGNNHLDIGVGTGFYLTHVPESSLISLMDLNEASLNAASTRAGESKIKHK
                     ISHDVFEPYPAALHGQFDSISMFYLLHCLPGNISTKSCVIRNAAQALTDDGTLYGATI
                     LGDGVVHNSFGQKLMRIYNQKGIFSNTKDSEEGLTHILSEHFENVKTKVQGTVVMFSA
                     SGKK"
     gene            complement(20000..20584)
                     /locus_tag="NBI74_001257"
     CDS             complement(20000..20584)
                     /locus_tag="NBI74_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000885581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HCD1931621.1"
                     /translation="MAFRMSEQPRTIKIYNLLAGTNEFIGEGDAYIPPHTGLPANSTD
                     IAPPDIPAGFVAVFNSDEASWHLVEDHRGKTVYDVASGDELFISELGPLPENVTWLSP
                     EGEFQKWNGTAWVKDTEAEKLFRIREAEETKNNLMQVASEHIAPLQDAADLEIATEEE
                     TSLLEAWKKYRVLLNRVDTSTAPDIEWPTNPVRE"
     gene            complement(20584..23544)
                     /locus_tag="NBI74_001258"
     CDS             complement(20584..23544)
                     /locus_tag="NBI74_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HCD1931622.1"
                     /translation="MAVKISGVLKDGTGKPVQNCTIQLKAKRNSTTVVVNTLASENPD
                     EAGRYSMDVEYGQYSVILLVEGFPPSHAGTITVYEDSRPGTLNDFLGAMTEDDARPEA
                     LRRFELMVEEVARNASAVAQNTAAAKKSASDAGTSAREAATHATDAAGSARAASTSAG
                     QAATSAQEASSSAGTASTKATEASKSAAAAESSKSAAATSAGAAKTSETNAAASQKSA
                     ATSASTATTKASEAATSARDAAASKEAAKSSETNASTSASSAASSATAAGNSVKAAKT
                     SETNARSSETAAEQSASAAADSETAAASSAREASIKAEEAAVSATAAGKSAESAASSA
                     STATTKAGEATEQASAAARSASAAKTSETNAKASETSAESSKTAAASSASSAASSASS
                     ASASKDEATRQASAARGSATTASTKATEAAGSAAAAAQSKSTAESAATRAETAAKRAE
                     DIASAVALEDASTTKKGIVQLSSATNSTSESQAATPKAVKAAYDLANGKYTVQDATTA
                     QKGIVQLSSATNSTSESQAATPKAVKAAYDLANGKYTAQDATTAQKGIVQLSSATNST
                     SETLAATPKAVKAANDNANGRVPSARKVNGKALSADITLTPKDIGTLNSTTMSFSGGA
                     GWFKLATVTMPQASSVVSITLIGGAGFNVGSPQQAGISELVLRAGNGNPKGITGALWQ
                     RTSTGFTNFAWVNTSGDTYDIYVAIGNYATGVNIQWDYTSNASVTIHTSPAYSANKPE
                     GLTDGTVYSLYTPSEQFYPPGAPIPWPSDTVPSGYALMQGQTFDKSAYPKLAAAYPSG
                     VIPDMRGWTIKGKPASGRAVLSQEQDGIKSHTHSASASSTDLGTKTTSSFDYGTKSTN
                     NTGAHTHSLSGSTNAAGNHSHRDGRRFNPSVFKDTYQYGYTSSGQNTWGVQGSVGMST
                     GWLANTSTDGNHSHSLSGTAASAGAHAHTVGIGAHTHSVAIGSHGHTITVNAAGNAEN
                     TVKNIAFNYIVRLA"
     gene            complement(23609..24208)
                     /locus_tag="NBI74_001259"
     CDS             complement(23609..24208)
                     /locus_tag="NBI74_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HCD1931623.1"
                     /translation="MRKVCAAILSAAICLAVSGAPAWASEHQSTLSAGYLHARTNVPG
                     SDDLNGINVKYRYEFTDTLGLVTSFSYAGDKNRQLTHYSDTRWHEDSVRNRWFSVMAG
                     PSVRVNEWFSAYAMAGVAYSRVSTFSGDYLRVTDNKGKTHDVLTGSDDGRHSNTSLAW
                     GAGVQFNPTESVAIDIAYEGSGSGDWRTDGFIVGVGYKF"
     gene            complement(24279..27692)
                     /locus_tag="NBI74_001260"
     CDS             complement(24279..27692)
                     /locus_tag="NBI74_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HCD1931624.1"
                     /translation="MGKGSSKGHTPRETKDNLKSTQLLSVIDAISEGPVEGPVDGLKS
                     VLLNSTPVLDSEGNTNISGVTVVFRAGEQEQTPPEGFESSGSETVLGTEVKYDTPITR
                     TITSANIDRLRFTFGVQALVETTSKGDRNPSEVRLLVQIQRNGGWVTEKDITIKGKTT
                     SQYLASVVVDNLPPRPFSIRMRRMTPDSTTDQLQNKTLWSSYTEIIDVKQCYPNTALV
                     GVQVDSEQFGSQQVSRNYHLHGRILQVPSNYNPQTRQYSGIWDGTLKPAYSNNMAWCL
                     WDMLTHPRYGMGKRLGAADVDKWALYVIGQYCDQSVPDGFGGTEPRITCNAYLTTQRK
                     AWDVLSDFCSAMRCMPVWNGQTLTFVQDRPSDKVWTYNRSNVVMLDDGAPFRYSFSAL
                     KDRHNAVEVNWIDPDNGWETATELVEDTQAIARYGRNVTKMDAFGCTSRGQAHRAGLW
                     LIKTELLETQTVDFSVGAEGLRHVPGDVIEICDDDYAGISIGGRVLAVNSQIRTLTLD
                     REITLPSSGTTLISLVDGSGNPVSVEVQSVTDGVKVKVSRVPDGVAGYSVWGLKLPTL
                     RQRLFRCVSIRENDDGTYAITAVQHVPEKEAIVDNGAHFDGDQSGTVNGVTPPAVQHL
                     TAEVTADSGEYQVLARWDTPKVVKGVSFMLRLTVAADDGSERLVSTARTAETTYRFTQ
                     LALGNYRLTVRAVNARGQQGDPASVSFRIAAPAVPSRIELTPGYFQITATPHLAVYDP
                     TVQFEFWFSETRITDIRQVETTARYLGTALYWIAASINIKPGHDYYFYIRSVNTVGKS
                     AFVEAVGRASDDAEGYLDFFKGKITESHLGKELLEKVDLTEDNASRLDEFSKEWQDAN
                     DKWNAMWGVKIEQTKDGKHYVAGIGLSMEDTEEGKLSQFLVAANRIAFIDPANGNETP
                     MFVAQGNQIFMNDVFLKRLTAPAITSGGSPPVFSLTSDGKLTAKNADISGSVNANSGT
                     LNNVTINQNCTIKGMLEATQVRGDFVKAVSKAFPKKVGTWGNTETPNGTVTVTISDDH
                     NFDRQIIIPPIIFNGIAYDDPGSGNNPGGTRYTGYGFEVRKNGVLIASRETKGAIPGS
                     YSAVIDMPSGGGSVTLEFKIFQKGNQGAGNITDCTVIVTKKAASGISIR"
     gene            complement(27753..28400)
                     /locus_tag="NBI74_001261"
     CDS             complement(27753..28400)
                     /locus_tag="NBI74_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HCD1931625.1"
                     /translation="MAATHTLPLASLGMARICLYGDLQRFGRRIDLRVKTGSEAIRAL
                     AMQIPAFRQKLSDGWYQVRIAGRDAGETELSARLNEPLANGAVIHIVPRLAGAKSGGV
                     FQVVLGAALIAVAWWNPVGWLGAAAVSGMYAAGASMILGGVAQMLAPKARTPTAASTD
                     NGKQNTYFSSLDNMVAQGNVLPVLYGEMRVGSRVVSQEISTADEGDGGQVVVIGR"
     gene            complement(28298..29041)
                     /locus_tag="NBI74_001262"
     CDS             complement(28298..29041)
                     /locus_tag="NBI74_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001396591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCD1931626.1"
                     /translation="MTQTESAILAHARRCAPAESCGFVVSTPEGERYFPCVNISGEPE
                     AYFRMSPEDWLQAEMQGEIVALVHSHPGGLPWLSEADRRLQVQSDLPWWLVCRGAIHK
                     FRCVPHLSGRRFEHGVTDCYTLFRDAYHLAGIEMPDFHREDDWWRHGQNLYLDNLEAT
                     GLYQVPLSAAQPGDVLLCCFGSSVPNHAAIYCGDSELLHHIPEQLSKRERYTDKWQRR
                     THSLWRHWAWHASAFTGIYNDLAAASTFE"
     gene            complement(29047..29745)
                     /locus_tag="NBI74_001263"
     CDS             complement(29047..29745)
                     /locus_tag="NBI74_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HCD1931627.1"
                     /translation="MQDIPQETHHETTRLTQSAQVVLWEIDLTVVGGERYFFCNEQND
                     KGEPVTWQGRQYQAYPIQGTGFELNGKGSAARPTLTVSNLHGMVTGMAEDLQSLVGGT
                     VVRRKVYARFLDAVNFVNGNSDADPEQEVISRWRIEQCSELSAVSASFVLSTPTETDG
                     AVFPGRIMLANTCTWTYRGDECGYSGPAVADEYDQPTSDITKDKCSKCLSGCKFRNNV
                     GNFGGFLSINKLSQ"
     gene            complement(29755..30084)
                     /locus_tag="NBI74_001264"
     CDS             complement(29755..30084)
                     /locus_tag="NBI74_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCD1931628.1"
                     /translation="METFHWKVRPDMNVVSEPKVVTVKLGDGYEQRRAAGLNNQLSIY
                     SVTIRVRKCEHPSLKAFLERHGGVRAFQWTPPYDWKPIRVVCRKWSASVGALWVTITA
                     DFEQVVA"
     gene            complement(30084..33149)
                     /locus_tag="NBI74_001265"
     CDS             complement(30084..33149)
                     /locus_tag="NBI74_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HCD1931629.1"
                     /translation="MDQIANLVIDLGIDAAEFKNEIPRIKNLLNGAASDAERSSARMQ
                     RFMERQTQAARQTTQAASSAATAASVHAQTVEKNAQAHERMAREVEKTRQRMEALSQK
                     MREEQAQAMALAEAQDKAAAAFYRQIDSVKQASAGLQELQRIQQQIRQARNSGGIGQQ
                     DYLALISEVTAKTRVLTQAEEEATRQKVAFIRQLKEQATRQNLSSSELLRAKAAQLGV
                     SSAAEVYIRKMEQAGKATHSLGLKSAAARQEIGVLIGELARGNLGALRGSGITLANRA
                     GWIDTLMSPKGMMLGGVIGGIAAAVYGLGKAWYDGQKEGEEFNRQLSLTGHYAGVTAG
                     QLWTLSRAISGNGITQHAAAGALAQVVGSGAFRGNDIGMVARAAAQMERSVGQSVSDT
                     INQFKRLKDDPVNAAKALDNELHFLTATQLEQIRVLGEQGRSSDAARIAMSALAEETG
                     RRTADIDNNLNALGSTLKYLSDLWSRFWDAAMNIGREDSLDEQIAALQEKVSRAKRLP
                     WTASSSQVEYDQQRLNDLQEKKRQKDLQDAKEQAERNYQEQQKRRNAENAALNRMNET
                     EAARHQREIARINSMQYADQAVRDAAIQRENERYEKALASGKKKTRETRNDEATRLLL
                     QYSQQQAQVEGQIAAARQSAGIATEKMTEAHKQLLALQQRISDLDGKKLTADEKSVLA
                     RKDELIQALTLLDVKQQELQKQTALNDLKKKTIQLTSQLAEEERAQRQQHDLDIATVG
                     MGDQQRQRYQVQLSLRQKYQQQLEQLRRDSEQKGTYNTDDYRKAEQALTESLNRQLNE
                     NRRYWQQLEVVQGNWKNGVLRAFQDFTVDADNTAGTAEQVFSSAFSNMGNGLATFVTT
                     GKLNFKSFTSSVLSDMAKILAQATMMKSIKGIGSVLGFDLSSLSLNANGGIYQSADLS
                     RYSGTVVNRPTFFAFAKGAGVMGEAGPEAILPLRRGADGKLGVVADIGGSGMAMFAPQ
                     YNIEINNDGTNGQIGPAALKVVYDLGKKAAADFMQQQARDGGRLSGAYR"
     gene            complement(33121..33450)
                     /locus_tag="NBI74_001266"
     CDS             complement(33121..33450)
                     /locus_tag="NBI74_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HCD1931630.1"
                     /translation="MQFVMRLAREFRRADWRRMLSEMSATELGEWGDYFRMQSFSDVW
                     MDAQFASLKALIVRMVSGSSDAAVADFSLLPEENGIPERTDEELMHLGEGISGGVRYG
                     PDSQPGH"
     gene            complement(33459..33845)
                     /locus_tag="NBI74_001267"
     CDS             complement(33459..33845)
                     /locus_tag="NBI74_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HCD1931631.1"
                     /translation="MFLKQGTFNYEKQSVVLSELSGLQRIEYLAFVQQRTAKFDAEEG
                     ELPEAERQIAFLRMGMDINAWLVSRSLWNAEQSQDVETLCASVITTWSYDALGTGAEM
                     VLSLSGMGAIENAGDLEHEVLTPEKS"
     gene            complement(33906..34649)
                     /locus_tag="NBI74_001268"
     CDS             complement(33906..34649)
                     /locus_tag="NBI74_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCD1931632.1"
                     /translation="MTTPNPLAKTKGAGTTFWMYTGKGDAFVNPLSDTDWLRLAMVKD
                     LQPGEMTADAEDDTYLDDEDADWKTTTQGQKSVGDTSATLAWRPGDSGQKKLVQLFDS
                     GEVCAFRIKYPNGTVDVFRGWLSSLGKTIASKDVMTRTVKISGVGRPYLAEEGTETVG
                     VTGLTVAPASASVKVGATTTLTFTVKPDGASDKAISVHSTDPQTATVTLNGLVATVKG
                     VKQGSVSIVGMTSDGDFVAVATVAVSAAG"
     gene            complement(34660..35061)
                     /locus_tag="NBI74_001269"
     CDS             complement(34660..35061)
                     /locus_tag="NBI74_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCD1931633.1"
                     /translation="MNRHTQIRQVVLARLREQCGDSATFFDGLPAFVDAQELPAVAVW
                     LSDAQYTGKMTDEDDWQAVLHIAVFIRAQAPDSELDMWMESTIFPALNDVPALSGLID
                     TLIPLGFNYQRDNEMATWAMAEITYQITYTN"
     gene            complement(35058..35636)
                     /locus_tag="NBI74_001270"
     CDS             complement(35058..35636)
                     /locus_tag="NBI74_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCD1931634.1"
                     /translation="MKGLENAIRNLNSLDTRMVPQASAWAINRVAQKAVSVATRQVAG
                     NTVAGDNQVKGIPLKLVRQRVRVFKASPSGKMTARIRVNRGNLPAIKLGTARVRLARR
                     GGKLQYRGSVLKVGKYLFRDAFIQQLANGRWHVMRRIDGKNRYPIDVVKIPLSGPLTQ
                     AFEDARDHIIAAEMPKQLGYALKQQLRLWLTR"
     gene            complement(35648..35923)
                     /locus_tag="NBI74_001271"
     CDS             complement(35648..35923)
                     /locus_tag="NBI74_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA breaking-rejoining protein"
                     /protein_id="HCD1931635.1"
                     /translation="MSDPFSRLAARMDAITVRKMGKTASINDVDMTVIPGETLAELNA
                     LSGPAVSLVVFSSGYRPRRGDRVVYDGQQWTVTRHERFNGKPMIFIE"
     gene            complement(35916..36239)
                     /locus_tag="NBI74_001272"
     CDS             complement(35916..36239)
                     /locus_tag="NBI74_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308857.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2190 family protein"
                     /protein_id="HCD1931636.1"
                     /translation="MAKNFVEEGKTVAIVASAAISSGDLVQVGDVFAVALTDIPQGET
                     GDGITEGVFMLPKLKTDDMKTGKKVYLKSGKVQLTNSGSDPLVGVVWADAGTSAEEVP
                     VKLNV"
     gene            complement(36326..38353)
                     /locus_tag="NBI74_001273"
     CDS             complement(36326..38353)
                     /locus_tag="NBI74_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308856.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease ClpP"
                     /protein_id="HCD1931637.1"
                     /translation="MPQRNDRSRSTPTTSPKNNSWFRMQAGHQSDADIYIYDEIGFWG
                     VTAKQFISDLNALGDITHINLHINSPGGDVFEGIAIFNALKTHGASITVYVDGVAASM
                     ASVIAMVGNPVIMPENTFMMIHKPFGFTGGDAEDMRTYADLLDRVEAVLLPAYAQKTG
                     KTTDEIAAMLADETWMSGAECLAQGFADQVTPAVKAMACIQSKRTEEFKKMPESIRNM
                     ITPPRNSAPRVQDDGPAASRTPVQAAAPVVDENSIRAQVLAEQKARVNGINDLFAMFG
                     GRYQSLQAQCLADPECSLEQAREKLLNEMGRESTPSNKNTPAHIYAGNGNFVGDGIRQ
                     ALMARAGFEKTERDNVYNGMTLREYARMSLTERGIGVSSYNPMQMVGAAFIHSTSDFG
                     NILLDVANKAILQGWEDAPETYEQWTRKGQLSDFKIAHRVGMGGFSALRQVREGAEYK
                     YVTTGDKQATIALATYGELFSITRQAIINDDLNMLTDVPMKLGRAAKSTIADLVYAIL
                     TSNPKISTDNVSLFDKAKHANVLESAAMDVASLDKARQLMRVQKEGERHLNIRPAFVL
                     VPTAMESVANQVIRSSSVKGADINAGIINPVKDFATVIAEPRLDDNSQTTFYLAASKG
                     SDTIEVAYLNGVDTPYIDQMEGFSVDGVTTKVRIDAGVAPVDHRGLVKCTA"
     gene            complement(38298..39806)
                     /locus_tag="NBI74_001274"
     CDS             complement(38298..39806)
                     /locus_tag="NBI74_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HCD1931638.1"
                     /translation="MAILDDVIGVFSPGWKAARLRSRAVIQAYEAVKTTRTHKARREN
                     RTADQLSQYGAVSLREQARYLDNNHDLVIGVFDKLEERVVGKNGIIVEPHPVLRNGAI
                     ARDLAAEIRTRWSEWSVSPEVTGQFTRPMLERLMLRTWLRDGEVFAQMVSGRINSLAP
                     SAGVHFWLEALEPDFIPMTSDESNRLNQGVFVDDWGRPEKYLVYKSRPVSGRQMETKE
                     VDAERMLHLKFVRRLHQMRGTSLLSGVLIRLSALKEYEDSELTAARIAAALGMYIRKG
                     DGQSYEPDGNGRKENERELTIQPGIIYDDLKPGEEIGMVKSDRPNPNLETFRNGQLRA
                     VAAGSRLSFSSTARNYNGTYSAQRQELVESTDGYLILQDWFIGAVTRPMYRAWLKQAV
                     ASGVIRLPRDLDRSSLYTAVYSGPVMPWIDPVKEAEAWKIQIRGGAATESDWVRAGGR
                     NPDDVKRRRKAEIDENRNLDLVFDTDPASDKGGSSAATKRQEPQHTDDQSEE"
     gene            complement(39806..40018)
                     /locus_tag="NBI74_001275"
     CDS             complement(39806..40018)
                     /locus_tag="NBI74_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001072975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1931639.1"
                     /translation="MNQNDIEAMIQRYTEAEMAVLDGKSVTFNGQQMTMENLSEIRQG
                     RQEWERRLAALITRRRGHPGYRLARF"
     gene            complement(40015..42114)
                     /locus_tag="NBI74_001276"
     CDS             complement(40015..42114)
                     /locus_tag="NBI74_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015987600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HCD1931640.1"
                     /translation="MGFSSARNLGRDISAGFSPPRRMPISEAVKKFMRVPKGAGNSVP
                     WDPELTPYIIEPMNCLASREYDAVIFVGPARTGKTIGLIDGWIVYTIVCDPSDMLVVQ
                     MTEDKAREHSKKRLDRTFRSSAAVKKRMSPRRNDNNVHDKTFRDGSFLKIGWPSVNIM
                     SSSDYRFVALTDYDRFPENIDSEGDGFSLASKRTTTFMSAGMTLVESSPGRDICDSKW
                     RRKSPHEAPPTTGILSLYNRGDRRRWYWSCPHCGEYFQPAMDAMTGYRNEPDPFKASE
                     AAYLLCPHCSGIITAEKKRELNSAGVWLREGQVIDRNGNVSGEPRRSRIASFWMEGPA
                     AAYQTWAQLVYKLLTAEQEYEATGSEETLRAVINTDWGLPYLPRASMEQRKSELLEQR
                     AEPVPSRSVPDGVNFLVATVDVQAGRHRRFVVQVTGYGSRGERWIIDRYNITQSLRSD
                     CDGESQRIDPASYPEDWDVLLTDVFHKSWPLASDPSQQMRLMAMAVDSGGEDGVTDNA
                     YKFWRRCRRDGLGKRIYLFKGDSIRRAKLISRTFPDNTGRTGRRAQAAGDVPLWLLQT
                     DALKDRVNNALWRDSPGPGYVHFPDWLGSWFYDELTYEERSSDGKWSKPGRGANEAFD
                     LMVYAEALVILHGYEKIRWPDAPEWASRETWLECVQDSTEPSSSPEPVSTPVKKQKRK
                     KTVTDDVNPWLTSGGWL"
     gene            complement(42123..42662)
                     /locus_tag="NBI74_001277"
     CDS             complement(42123..42662)
                     /locus_tag="NBI74_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000421825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1441 family protein"
                     /protein_id="HCD1931641.1"
                     /translation="MELLMSNVSGIGDAYYWSVFKIAEAFGLHRDTVKKRLLAANTPV
                     AATIRGNPVYALQHVGPALFSVKHEAADSVHDPSRMEPKERKDWYQSENERIKLEKEQ
                     RKLIPVDEVVIVYSSMRKAVVQVLETIPDVLERDCALTPQAVGVVQQAIDDLRYTLQE
                     KSYEACAAELIPDEEGESL"
     gene            complement(43223..43429)
                     /locus_tag="NBI74_001278"
     CDS             complement(43223..43429)
                     /locus_tag="NBI74_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1931642.1"
                     /translation="MNKEQSADELSLDLIRVKNMLNSTISMSYPDVVIACIEHQVSLE
                     AFRAIEAALVKHDKNSKDYSLVVD"
     gene            43730..44140
                     /locus_tag="NBI74_001279"
     CDS             43730..44140
                     /locus_tag="NBI74_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309653.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1398 domain-containing protein"
                     /protein_id="HCD1931643.1"
                     /translation="MAQAAIFKEIFDQVRKDLNCELFYSELKRHNVSLYIYYLATDNI
                     HIVLENDNIVLVKGLKKVVNVKFSRNKHLIETSYNKLKSKEITFQQYRENLAKAGVFR
                     WVTNIQEHQRYYYAFDNSLLFTESIQKTTQILPR"
     gene            complement(44292..44465)
                     /locus_tag="NBI74_001280"
     CDS             complement(44292..44465)
                     /locus_tag="NBI74_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416068.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module toxin, GnsA/GnsB family"
                     /protein_id="HCD1931644.1"
                     /translation="MNIENLKTKAEADISEYITKKIIELKKKTGKEVTSIQFTAREKM
                     TGLESYDVKINLI"
     gene            complement(44637..44792)
                     /locus_tag="NBI74_001281"
     CDS             complement(44637..44792)
                     /locus_tag="NBI74_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1931645.1"
                     /translation="MREYPNGEKTHLTVMAAGFPSLTGDHKVIYVAADRHVTSEEILE
                     AAIRLLS"
     gene            complement(44872..44937)
                     /locus_tag="NBI74_001282"
     CDS             complement(44872..44937)
                     /locus_tag="NBI74_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1931646.1"
                     /translation="MKAPSGAFLLGIYSMDTHILR"
     gene            complement(44940..>45061)
                     /locus_tag="NBI74_001283"
     CDS             complement(44940..>45061)
                     /locus_tag="NBI74_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HCD1931647.1"
                     /translation="VSDMNPFGAKCIFCKSMMITFDNISQYLNASRLSLDLKK"
BASE COUNT        10867 a        12302 c        10747 g        11145 t
ORIGIN      
        1 gatgaactca gaaaaatcta taaagaacgt cgataccctt tcattataag attcacatac
       61 aatctgtctt tgccaaagag acccaatcgt gctattttaa tagatcatgt ggggctaaat
      121 ggttcgcgtg cattccgatg gagtcacttt aaactcacaa atgagcagtt cttaaagatc
      181 atcgagttag gcaagataaa tgaaagtttt attatccatt aagcctgagt ttgcagaaaa
      241 aatattgaac ggaacaaagc ggttcgagtt tcgtaaaggt atattcaaaa atccgcaaat
      301 tagcaccgtt gttatttatg ccacgatgcc attaggtaaa gttgttggtc aattccgtat
      361 tgaatcaata ctaagtgacg aaccggaatc tctttggaaa aagacggaaa aacacgcagg
      421 tatttctaag caattttatg actcatatta ttcaggtaga gaaaaggcct acgcaataaa
      481 aattggtgaa gtggaaagat ataaagaacc aattcctatc tctgctctag gtagtaatat
      541 taagccacca caatcatatc tttacctacc tgcgtaagaa tcccggccac cgtgccgggt
      601 tttcttttgt cccctcatca cacaaaccgt tcgaaaaacc accacatcct cccttcagtt
      661 atcgctatgc gatacaagtc acaaaattaa ttctttttgc tatcaaacag ttaatatcaa
      721 aacacatcaa tcaatagcaa taagtattga tacagccaat aacaatagct attatcacca
      781 tgtcgcaaca acacaacgat acggcaatca cctgattcac cgttgcgatg accgcttaga
      841 tccgcagctt gaatttcagc aggctccggg gagtgcgagg ggtgaagcgg acgcgtgaac
      901 gtcggtgtga ccagctgaaa tcaactcaac acctcatacc tcagtcgctt caacgaggcg
      961 gcttagttat gacaaccggc ggccatccac cgcctgaata cgcgcagaag tctctatatg
     1021 ttcagcagcc cagcttacgg gcaggagttt ttatggttca tcaacattac ggaacgcaga
     1081 ccgttaatcg cggtgcggtc atgccaggaa tgctggtcaa acacaaagat ggtacctgga
     1141 ctgcatcagc taatttacgc ggacggcttt atctgcatcg cggcatcgag cgcacttata
     1201 cccgtgattt gctcgtggaa gtttttctcg acggacgcgg caacggcctg aatcactaat
     1261 cccctttcct gtttacctaa tcagcccggc atttcgcagg cgatattttc acagccattt
     1321 tcaggagttc agccatgaac gcttattaca ttcaggatcg tcttgaggct caaagctggg
     1381 cgcgtcacta ccagcagatc gcccgtgaag agaaagaggc agaactggca gacgacatgg
     1441 aaaaaggtct tccacagcac ctgtttgaat cactctgcat cgatcatttg caacgctgcg
     1501 gggccaacaa aaaagccatt acccgtgcgt ttgatgacga tgttgagttt caggagcgca
     1561 tggcagaaca catccggtac atggttgaaa ccattgctca ccaccatgtt gatattgatt
     1621 cagaggtata aaacggatga gtacagcact cgcaacgcta gccgggaagc tggctgaacg
     1681 tgtcggcatg gattctgtcg acccacagga actgatcacc actcttcgtc agacggcatt
     1741 taaaggtgat gccagcgatg cgcagttcat cgcattgctg atcgtcgcca accagtacgg
     1801 ccttaatccg tggacgaaag aaatttacgc cttccctgat aagcagaacg gcatcgttcc
     1861 ggtggtgggc gttgatggct ggtcccgcat catcaatgaa aaccagcagt ttgatggcat
     1921 ggactttgag caggacaatg aatcatgtac gtgccggatt taccgcaaag accgcaatca
     1981 tccgatctgc gttaccgaat ggatggatga atgccgccgc gaaccattca aaactcgcga
     2041 aggcagagaa atcacggggc cgtggcagtc gcatcccaaa cggatgttac ggcataaagc
     2101 catgattcag tgtgcccgtc tggccttcgg atttgctggt atctatgaca aggatgaagc
     2161 cgagcgcatt gtcgaaaata ctgcatacac tgcggaacgt cagccggaac gcgacatcac
     2221 tccggttaac gatgaaacca tgcaggagat taacactctg ctgatcgccc tggataaaac
     2281 atgggatgac gacttattgc cgctctgttc ccagatattt cgccgcgaca ttcgcgcatc
     2341 gtcagaactg acacaagccg aagcagtgaa agctcttgga ttcctgaaac agaaagccac
     2401 tgagcagaag gtggcagcat gacaccggac attatcctgc agcgtaccgg gatcgacgtg
     2461 agagctgtcg aacaggggga tgatgcatgg cacaaattac ggctcggcgt catcaccgct
     2521 tcagaagttc acaacgtgat agcaaagccc cgctcaggaa agaagtggcc tgacatgaaa
     2581 atgtcctact tccacacctt gctggctgag gtttgcaccg gtgtggctcc ggaagttaat
     2641 gctaaggcgc tggcctgggg aaaacagtac gagaacgacg ccagaaccct gtttaaattc
     2701 acttccggcg tgaatgttac tgaatccccg atcatctatc gcgacgaaag tatgcgcacc
     2761 gcctgctctc ccgatggttt atgcagtgac ggcaatggcc ttgagctgaa atgcccgttt
     2821 acctcccggg atttcatgaa gttccggctc ggtggtttcg aggccataaa gtcggcttac
     2881 atggcccagg tgcagtacag catgtgggtg acgcgaaaag atgcctggta ctttgccaac
     2941 tatgacccgc gtatgaagcg tgaaggactg cattatgtcg tggttgagcg ggatgaaaag
     3001 tacatggcga gttttgacga gatggtgccg gagttcatcg aaaaaatgga cgaggcactg
     3061 gctgaaattg gttttgtatt tggggagcaa tggcgatgac gcatcctcac gataatatcc
     3121 gggtaggcgc gatcactttc gtctactccg ttacaaagcg aggctgggta tttcccggcc
     3181 tttctgttat ccaaaatcca ctgaaagccc agcggctggc tgaggagata aataataaac
     3241 ggggggctgt atgcacaaag catctcccgt tgagttaaga acgagtatcg agatggcaca
     3301 tagcctcgct caaattggag tcaggtttgt gccaatacca gtagaaacag acgaagaatt
     3361 tcatacgtta gccacatccc tttcacaaaa gctggaaatg atggtggcga aagcagaagc
     3421 agatgagaga gaccaggtat gacaaccact gaatgcattt ttctggcagc gggcttcata
     3481 ttctgtgtgc ttatgcttgc cgacatggga cttgttcaat gacacctcag caagaaaacg
     3541 cccttcgcag tattgcccgt caggctaatt atgaaatcaa aaaagccaga cagcagtttc
     3601 cggataaaaa cgtcgatgac atttgccgta gcgtactgaa gaagcaccgc gaaacggtaa
     3661 cgctgatggg attcacaccg actcatttaa gcctggcgat cggcatgtta aacggcgtct
     3721 ttaaggaacg gtgaacatga aaaataaaat catcatggag ctacaggctc cttttttatt
     3781 attcgcattc accctcaagc gtattaacca acaattcagg gattaatgaa agatggcaga
     3841 catcattgat tcagcatcag aaattgaaga attacagcgc aacacagcaa taaaaatgcg
     3901 ccgcctgaac caccaggcta tatctgccac tcattgttgt gagtgtggcg atccgataga
     3961 tgaacgaaga cgcctggccg ttcagggttg tcggacttgt gcaagttgcc aggaggagat
     4021 cgaacttaag aacaaacaat ggggactgtg atggcctcaa agcagcaaat ttcaacatcg
     4081 tccaactgag gtgtaaaaat gttcagaatc atttttccta acacctggta cgtcgaccac
     4141 cacggcactc cctgcaaaat cctgcgttct acccacaaca aagttcacta catccgaaaa
     4201 ggcagaacat gtatcgccag catgttccgc tttaatcatg actttgaacc tgtgaataaa
     4261 gctgatgcag atcggatagc agaagagatc gaaacggcag aacacattaa gaagttacgt
     4321 gccatacgca ggaaatagaa aatttgataa attcaatact gcatttctca gcattaaatt
     4381 tatctctatg accagtcaag agatgtacct gccatgagct taatatcatg tcagatatat
     4441 cggtcacaaa ctccctcagc agctaagagg aggacaaatg tctcgactaa tcactttaca
     4501 ggactgggct aaagaagaat ttggggactt agcaccaagt gagcgagttc tgaaaaaata
     4561 cgcgcaaggg aaaatgatgg ccccacccgc tataaaagtt ggtcgctact ggatgattga
     4621 ccgaaattcc cgttttgtag gaacgcttgc agaaccgcaa ctcccaataa acgcaaaccc
     4681 aaaactccaa cggataatcg ctgatggctg ctagaccccg atctcacaaa atctctatac
     4741 ccaatttata ttgcaaatta gataagcgaa ccggaaaggt atattggcaa tacaaacatc
     4801 cactatccgg tcgttttcat agcttaggaa ctgatgagaa tgaagcaaaa caagttgcta
     4861 ctgaagcaaa taccattatt gctgaacaac gtacccgaca aatattaagc gtcaatgagc
     4921 gtctggaaag aatgaaaggc aggcgctcag acattacggt gacagaatgg cttgataaat
     4981 atatttctat ccaggaggac aggctgcaac ataatgaact aagacccaac tcctatcggc
     5041 aaaaaggcaa acccattcgt cttttccgtg agcattgtgg aatgcaacac ctcaaggata
     5101 ttaccgcact tgatattgcc gaaataattg atgctgtaaa ggctgaaggt cataacagga
     5161 tggcgcaagt cgtgagaatg gtgttaatcg acgtcttcaa agaagcacaa cacgcaggac
     5221 atgttccgcc aggatttaac ccagcgcagg caacaaaaca accgcgaaat cgagtaaacc
     5281 gccaaagatt atcactgccc gaatggcagg caatatttga cagcgtaagc agacggcagc
     5341 cctatttaaa atgcgggatg ctacttgctc ttgtcactgg acaacgttta ggcgatatct
     5401 gcaatttgaa attctctgat atctgggacg acatgttgca cattactcag gaaaaaaccg
     5461 gttcaaaact tgctattccg cttaacctga aatgcgatgc tctgaatatt acccttcgtg
     5521 aagttatatc tcagtgcagg gatgctgttg ttagtaaata tctggtccat taccgtcaca
     5581 caacctctca agcaaacaga ggagaccagg tttctgcgaa tactctgaca acggctttta
     5641 aaaaggccag ggaaaaatgt ggcataaaat gggagcaagg aactgcgccc acatttcatg
     5701 agcagcgatc tctgtcagaa cggttatatc gggaacaggg tctggatacg caaaagttgt
     5761 taggccataa atccagaaaa atgaccgacc gatacaatga tgatcgtggt aaagactgga
     5821 ttatcgtaga tatcaaaaca gcatagaaaa tagccagttt tggggaaggg ttttggggaa
     5881 agttttgggg aagattttac atcatcataa aacaacgggc gtgttatacg cccgtttcaa
     5941 tatttaacac atgtagtgat tacatattct tgatgatcgc atcaccaaac tctgaacatt
     6001 tcagcagttt agcgccttcc atcagacgtt cgaagtcata ggttacggtc ttggcgttga
     6061 ttgcgccttc cataccttta acaatcaggt cagccgcttc agtccaaccc atatggcgca
     6121 gcatcatctc agcggagaga ataatagagc ctgggtttac tttgtcctgt ccggcatatt
     6181 tcggcgcagt accgtgggtg gcttcaaaca gggcgcattc gtcaccgatg tttgcgccag
     6241 gggcgatacc gataccacca acctgcgctg ccagggcgtc agaaatgtag tcaccgttca
     6301 ggttcataca ggcgataaca tcatattcag ccggacgcag caggatctgt tgcaggaatg
     6361 catcagcaat cacgtcttta atgacgatct ctttgccggt attcgggttt ttaactttca
     6421 gccacgggcc gccgtcgatc agttcaccgc caaattcttc acgcgccagt tggtagcccc
     6481 agtctttaaa cgcgccttcg gtgaacttca tgatgttgcc tttgtgcacc agggtcacag
     6541 agtcacgatc gttagcaatt gcgtattcga tcgctgcacg aaccagacgt ttggtgcctt
     6601 cttccgaaca cggcttaata ccgataccgc aatgttccgg gaagcgaatt ttcttcacgc
     6661 ccatctcttc acgcaggaat ttaatcactt tctcagcgtc ggcagagtct gctttccatt
     6721 cgatacccgc ataaatgtct tccgagtttt cacggaagat aaccatatcg gtcagttcag
     6781 ggtgtttaac cgggcttgga gtgccctgat agtaacgtac cggacgcagg cagatgtaga
     6841 gatccagttc ctggcgcagg gcaacgttca gagagcgaat accgccacca accggagtgg
     6901 tcagcgggcc tttaatggca acgcgatatt cacgaatcag atcaagggtt tcagcaggca
     6961 accagacgtc ctgaccataa acctgtgtgg atttttcacc ggtgtaaatt tccatccagg
     7021 agattttacg ctcgccttta taggctttct cgactgcagc gtcgaccact ttcagcatgg
     7081 ctggggttac atctacaccg attccatcac cttcaatgta agggataatc ggattttcag
     7141 gaacgttgag tttgccgttt tgcagggtga tcttcttgcc ttgtgccgga acaactactt
     7201 tactttccat tcacctctcc ttcgagcgct actggtttgc tcgtctgcca ccacgttgca
     7261 tatgcgtttg cgtcctgcga tacggatgct ttagagcaat tttttgttaa tgatttgtaa
     7321 ttagcttgtc aatactaccc tattgtttgc cgtcatgaaa gatgcgcgtt attaggctat
     7381 aatgcggcaa ttcataatct ctgaaaatac catgcaaaaa acttctttta gaaatcacca
     7441 ggttaagcga ttcagctcgc aacgttctac caggcgtaaa cctgacaacc agcccacgcg
     7501 tgtgatcctg ttcaataaac cctacgatgt tcttccgcag ttcaccgatg aagccggacg
     7561 caaaacatta aaagaattca tcccggttca gggggtgtat gcggcaggtc gtctcgaccg
     7621 cgacagcgaa gggttactgg tgctgaccaa taacggcgcg ttgcaggcgc gtttaaccca
     7681 gccgggtaaa cgcaccggaa aaatctatta tgtgcaggtg gaaggtattc ccacacaaga
     7741 cgcacttgaa gccttgcgca atggcgtaac cttaaatgat ggccctaccc tgcccgcagg
     7801 cgcggagctg gttgaagaac ccgcgtggtt atggccgcgg aatccaccaa ttcgtgagcg
     7861 caaaagtatt cccaccagct ggctgaagat caccttatat gaaggacgta atcgccaggt
     7921 gcgccgcatg accgcccatg ttggtttccc cacgctgcga ctgattcgct atgcgatggg
     7981 ggattactct ttggataatc ttgccaatgg cgaatggcga gacgcgacag attaaggatg
     8041 caaaatgttt aaaccgcacg ttaccgttgc ttgcgtggtg cacgcagaag gcaaattttt
     8101 agtcgttgaa gagacgatta atggtaaagc gttatggaac caacctgccg ggcacctgga
     8161 agccgatgaa accttagtgg aagccgccgc ccgtgagctg tgggaagaaa ccggcattag
     8221 cgcgcagccg caacacttta ttcgtatgca tcagtggatt gcgcccgata aaacgccgtt
     8281 tttgcgtttc ctctttgcca ttgagcttga gcaaatatgc ccgacgcagc ctcatgacag
     8341 cgatatcgac tgctgccgtt gggtcagcgc cgaagaaatt ttacaggcgt caaatcttcg
     8401 ttcgccgctg gtggcggaaa gtattcgttg ttatcaaagc gggcaacgct atccgctgga
     8461 gatgattggc gattttaact ggccttttac aaagggtgtc atctaaaagg ctgcgtgata
     8521 gaatacgccg ccttgaagtt caatgtcgtg agtgatccaa tgtctgaaac cgcaaaaaaa
     8581 gtaatcgtcg gcatgtccgg cggtgtcgat tcctccgttt ctgcctggct gttacaacaa
     8641 cagggatatc aggtcgaagg cctgtttatg aagaactggg aagaagacga cggtgaggaa
     8701 tattgcacag cggcagcgga tctggctgat gcccaggctg tctgcgacaa gctcggcatt
     8761 gaactgcaca ccgttaactt tgctgccgag tactgggaca acgtcttcga actgttcctt
     8821 gccgaatata aagccggtcg cacgccgaat ccggatattc tgtgcaacaa agagatcaaa
     8881 tttaaagctt tcctcgaatt tgccgccgaa gatttaggtg ccgattatat cgctaccggt
     8941 cattacgtac gtcgggcaga tgtcgatggc aagagccgcc tgctgcgtgg tctggacagc
     9001 aataaagacc agagctactt cctttatacg ctcagccatg agcagattgc gcaaagcctg
     9061 ttcccggtcg gcgaactgga aaaaccgcag gtgcgtaaga ttgctgaaga tcttggtctg
     9121 gtcaccgcga agaaaaaaga ctctaccggc atttgcttca ttggcgaacg taaattccgc
     9181 gagttcctgg gccgttatct cccggcgcaa ccgggcaaaa tcattaccgt cgatggcgat
     9241 gaaattggtg agcaccaggg gctgatgtat cacaccctcg gtcagcgtaa aggtctgggt
     9301 atcggtggca ccaaagacgg caccgaagaa ccgtggtatg tggtcgataa agacgtcgaa
     9361 aacaacattc tgattgttgc gcaaggccat gaacacccgc gtctgatgtc tgtcggtctg
     9421 attgcccagc agctgcactg ggtcgatcgc gaaccgttca ccggtactat gcgttgcaca
     9481 gtaaaaaccc gctatcgcca gaccgacatc ccgtgcaccg tcaaggcgct ggacgatgat
     9541 cgcattgaag tgattttcga tgagccggtt gccgccgtga cgccgggcca gtctgccgtt
     9601 ttctataacg gtgaagtgtg cctcggtggc ggtattattg agcagcgtct gccgctgccg
     9661 gtctgattat tatctttact taaaacaagg aagcagtgaa cgtggcaaag aattactatg
     9721 acatcaccct cgccctggcc ggtatttgtc agtcggcacg cctggtgcaa caactcgctc
     9781 accaggggca ttgtgatggc gatgcgctac acgtctcact caacagtatt atcgatatga
     9841 accccagctc aacgctggcg gtttttggcg gcagcgaagc caacctgcgc gtcgggctgg
     9901 aaaccttgct cggcgtactc aatgccagca gtcgccaagg cttaaacgcc gaattaaccc
     9961 gctacacact cagtctgatg gtgcttgagc gcaaactctc ctcagcgaaa ggtgcgctcg
    10021 acactctggg caaccggatc aacggcctgc aacgccagct cgaacacttc gatttacagt
    10081 ccgaaacgct gatgagcgcg atggctgcca tctatgttga tgttatcagc ccgcttggcc
    10141 cgcgcattca ggtcacgggt tcccctgctg tactgcaaag cccacaggtg caggcgaaag
    10201 ttcgcgcaac cctgctggca ggcattcgcg ccgccgtgct ctggcaccag gtcggcggcg
    10261 gtcgtctgca actgatgttt tctcgtaatc gcctgaccac tcaggcaaaa caaattcttg
    10321 ctcatttaac cccggagttg tgatctatgg aattatcctc actgaccgcc gtttcccctg
    10381 tcgatggacg ctacggcgat aaagtcagcg cgctgcgcgg gattttcagc gaatacggtt
    10441 tgctgaaatt ccgtgtacaa gttgaagtac gttggctgca aaaactggcc gcgcacgcag
    10501 cgatcaagga agttcctgct tttgctgccg acgcaatcgg ttaccttgat gcaattgtcg
    10561 ccagtttcag cgaagaagat gcagcacgca tcaaaaccat cgagcgtacc actaaccacg
    10621 acgttaaagc ggttgagtat ttcctgaaag aaaaagtggc ggatatcccg gaactgcacg
    10681 cggtttctga attcatccat tttgcctgta cttcggaaga tatcaataac ctctcccacg
    10741 cattaatgct gaaaaccgcg cgtgatgaag tgatcctgcc gtactggcgt caactgattg
    10801 atggcattaa agatctcgcc gttcagtatc gcgatatccc gctgctgtcc cgtacccacg
    10861 gtcagccagc cacgccgtca accattggta aagagatggc taacgtcgcc taccgtatgg
    10921 agcgccagta ccgccagctt aaccaggtgg agatcctcgg caaaatcaac ggcgctgtcg
    10981 gtaactacaa cgcccacatc gccgcttacc cggaagttga ctggcatcag ttcagcgaag
    11041 agttcgtcac ctcgctgggt attcagtgga acccgtacac cacccagatc gaaccgcacg
    11101 actacattgc cgaactgttt gattgcgttg cgcgcttcaa caccattctg atcgactttg
    11161 accgtgacgt ctggggttat atcgccctta accacttcaa acagaaaacc attgctggtg
    11221 agattggttc ttccaccatg ccgcataaag ttaacccgat cgacttcgaa aactccgaag
    11281 ggaacctggg cctttccaac gcggtattgc agcacctggc aagcaaactg ccagtttccc
    11341 gctggcagcg tgacctgacc gactccaccg tgctgcgtaa cctcggcgtg ggtatcggtt
    11401 atgcgctgat tgcgtatcaa tccaccctga aaggcgtgag caaactggaa gtgaaccatg
    11461 accatctgct ggaggagctg gatcacaact gggaagtgct ggctgagcca atccagacag
    11521 ttatgcgtcg ctatggcatc gaaaaaccgt acgagaagct gaaagagctg actcgcggta
    11581 agcgcgttga cgccgaaggc atgaagcaat ttatcgacgg tctcgcgctg ccagaagaag
    11641 agaaagcccg cctgaaagcg atgacgccgg ctaactatat tggtcgcgcc atcaccatgg
    11701 ttgatgagct gaaataagcc tcaaatcagt gccggatggc gatgctgtcc ggcctgctta
    11761 ttaagattat ccgcttttta ttttttcact ttacctcccc ttcccgctgg tttatttaat
    11821 gtttaccccc ataaccacat aatcgcgtta cactatttta ataattaaga cagggagaaa
    11881 taaaaatgcg cgtactggtt gttgaagaca atgcgttgtt acgtcaccat cttaaagttc
    11941 agatacagga tgctggtcat caggtcgatg acgcagaaga tgccaaagaa gccgattatt
    12001 atctcaatga acatttaccg gatattgcga ttgtcgatct cggattgcct gacgaggatg
    12061 gtctgtcact gattcgccgc tggcgtagca atgatgtttc actgccgatt ctggtattaa
    12121 ccgcccgtga gagctggcag gacaaagtcg aagtattaag tgccggtgct gatgattacg
    12181 tgactaaacc gtttcatatt gaagaggtga tggcgcgaat gcaggcatta atgcggcgta
    12241 atagcggtct ggcttcacag gtcatttcgc taccaccgtt tcaggttgat ctctcccgcc
    12301 gtgaattatc tattaatgac gaagtgatca aactgaccgc gttcgaatac accattatgg
    12361 aaacgttgat acgcaataat ggcaaagtgg tcagcaaaga ttcgttaatg ctacaactct
    12421 acccggatgc ggagctgcgg gaaagccata ccattgatgt actgatggga cgtctgcgca
    12481 aaaaaattca ggcacaatat ccgcaagaag tgattaccac cgttcgcggc cagggctatc
    12541 tgttcgaatt gcgctgatga aaaaattact gcgtcttttt ttcccgctct cgctgcgggt
    12601 acgttttctg ttagcaacgg cagcagtagt attggtgctt tcgcttgcct acggaatggt
    12661 tgcgctgatc ggttatagcg tcagtttcga taaaaccacg tttcggctgt tacgtggcga
    12721 gagcaatctg ttctataccc ttgcgaagtg ggaaaacaat aagttgcatg tcgagttacc
    12781 cgaaaatatc gacaagcaaa gccccacgat gacactaatt tatgatgaga acgggcagct
    12841 tttatgggcg caacgtgacg tgccctggct gatgaagatg atccagcccg actggctaaa
    12901 atcgaacggt tttcatgaaa ttgaagcgga tgttaacgat accagcctct tgctgagtgg
    12961 agatcattcg atacagcaac agttgcagga agtgcgggaa gatgatgacg acgcggagat
    13021 gacccactcg gtggcggtaa acgtctaccc ggcaacatcg cggatgccaa agttaaccat
    13081 tgtggtggtg gataccattc cggtggagct aaaaagttcc tatatggtct ggagctggtt
    13141 tatctatgtg ctctcagcca atctgctgtt agtgatcccg ctgctgtggg tcgccgcctg
    13201 gtggagttta cgccccatcg aagccctggc aaaagaagtc cgcgaactgg aagaacataa
    13261 ccgcgaattg ctcaatccag ccacaacgcg ggaactgact agtctggtac gaaacctgaa
    13321 ccgactgtta aaaagtgaac gcgaacgtta cgacaaatat cgtacgacgc tcaccgacct
    13381 gacccatagt ctgaaaacgc cactggcggt gctgcaaagt acgctgcgtt ctctgcgtag
    13441 tgagaagatg agcgtcagtg atgctgagcc ggtaatgctg gagcaaatca gccgcatttc
    13501 acagcaaatt ggctactacc tgcatcgtgc cagtatgcgc ggcgggacat tgcttagccg
    13561 cgagctgcat ccggtcgccc cactgctgga caatctcacc tcggcgctga acaaagtgta
    13621 tcaacgcaaa ggggtcaata tctctctcga tatttcgcca gagatcagct ttgtcggtga
    13681 gcagaacgat tttgtcgagg tgatgggcaa cgtgctggat aatgcctgta aatattgcct
    13741 cgagtttgtc gaaatatctg caaggcaaac agacgagcat ctctatattg tggtcgagga
    13801 tgatggcccc ggtattccat taagcaagcg agaggtcatt ttcgaccgtg gtcaacgggt
    13861 tgatacttta cgccctgggc aaggtgtagg gctggcggta gcccgcgaaa tcaccgagca
    13921 atatgagggt aaaatcgtcg ccggagagag catgctgggc ggtgcgcgga tggaggtgat
    13981 ttttggtcgc cagcattctg caccgaaaga tgaataaata tgtccatact tcacgcatta
    14041 cgttaagcat ccgttataat cggttgcaga taccagcctg tggatgctta acatggaata
    14101 ccaactcact cttaactggc ccgattttct tgaacgtcac tggcagaaac gtccggtggt
    14161 gttaaaacgc ggctttaata attttattga cccgatctct ccagacgagt tggcgggttt
    14221 ggcgatggaa agcgaagtcg acagtcgact ggtcagtcat caggatggca aatggcaggt
    14281 cagccacggt ccattcgaaa gctacgatca tctcggtgaa actaactggt cattgttagt
    14341 gcaagcagtg aaccactggc atgagccgac cgccgcgcta atgcgaccgt tccgtgaact
    14401 accggactgg cgtattgatg atctgatgat ctctttttct gtaccgggcg gcggcgtcgg
    14461 cccgcatctc gatcagtacg acgtgtttat cattcagggt accggtcgtc gtcgctggcg
    14521 agtgggcgaa aagctgcaaa tgaaacagca ctgccctcac ccggatctgt tacaggtcga
    14581 tccgttcgaa gccatcatcg atgaagagct ggagcctggc gatattcttt atattccgcc
    14641 aggattcccg catgaaggct acgcgctgga aaatgcgatg aactattccg tgggttttcg
    14701 cgcgccaaat acgcgggaac tgattagcgg atttgccgat tatgtgctgc aacgtgaact
    14761 gggcggcaac tactacagcg atcctgatgt tccacctcgc gctcatcctg cggacgttct
    14821 gccgcaagag atggataaac tgcgtgagat gatgctcgaa ttgatcaacc agccagaaca
    14881 ttttaagcaa tggtttggcg agtttatatc ccagtcacgt catgaactgg atatcgcgcc
    14941 gccagagccg ccttatcagc cggatgaaat ctacgatgcg ctgaaacaag gtgatgtgct
    15001 ggtgcgcctg gggggtctgc gcgtattgcg cattggcgac gacgtgtatg ccaatggtga
    15061 gaagatcgat tccccgcacc gtccggcact ggatgcgctc gccagcaaca ttgcgctgac
    15121 cgcggagaat tttggcgatg cgctggaaga tccgtcattc ctcgcgatgc tcgcggcgct
    15181 ggtcaatagc ggatactggt tcttcgaagg gtaagtttgg atttaagccg gatgcggcat
    15241 cacatgccgc atcccttttc gcttacttcc gttgcgccgt caactcggca atacggacga
    15301 tcacctgcac cgctttttcc ataccttcca gagtcacaaa ctcatgctta ccatgatagt
    15361 tgtaaccgcc agtgaacagg ttcgggcacg gtaatcccat aaacgacaac tgcgcgccgt
    15421 cggtaccgcc gcggatcggt ttcagttccg gttcaatatc gcagtcacgc atcgcctgct
    15481 gggcgatatc gagaatatgc ggatgctcaa ccactttctc gcgcatattg tagtaactgt
    15541 cttcaatcac cagttcaatg tagcaatcag gatgtaaccc tttgcccact tttttggcga
    15601 tctccatcat tttacgttta cgcgcttcaa actgtttacg gtcgaaatca cggatgatgt
    15661 agtgcatatc ggcccgttca acggtgcctt tcatgctcgc caggtgatag aaaccttcat
    15721 agccttctgt catttccggg ctttcatccg ccggaacttc cgcatgaata cgtgccgcca
    15781 gcgacagcgc atttaccatc actcctttcg ccgtgcccgg gtgaacattg ttaccgacaa
    15841 ttttgatatt gaccgacgcg gcgttgaagt tttcaaactc cagttcacct acgccaccac
    15901 catcaacggt gtaagcccag cgggcatcga aggcgtcaac atcaaaatgt ttcgcccctt
    15961 tgcccacttc ttcatccggg gtaaaggcga cgcgaatatc accatgcgga atgttttttt
    16021 gttgcaatac cgccagcgcg gtcatgattt ctgcaatacc tgctttgtca tcggcaccta
    16081 acaaggtttt accatcggtg gtaatcagcg tctgacccag tagctgatgc agcaccggga
    16141 acataaccgg tgataaaact tcatcgccga tacccagcgc aatatcgcca ccgcgatagt
    16201 tttcaacaat ttgcggattc acatttttgc cgctgcaatc cggtgaggta tccacatgag
    16261 aaataaagcc aatcgccggg atatcgccag ggacgttagc cggtaacgtc gccatcaaag
    16321 tgcccttctc acttaaggtc acattgataa gccccatctc ttcgagctgc tctttcagca
    16381 gatgcaataa cttccattgg ccttccgtgc tgggaacctg tctcatccct gcctttgatt
    16441 gggtatcaag agacacgtag ttcaaaaatc gctcaagtag tttatccatg tagtcaccct
    16501 cactttttgt gacaacatta ttaagaagca agaaaagaca aatattgcgt caggtcactt
    16561 ttagccctgc aagcgggaat atttatcagc attaccttaa tgaatataaa gctaagccag
    16621 taattcacaa caaagattgg cgattcaagc ggtttgccgt agaattaccg ccctcattaa
    16681 gagtcaccaa ggtggttaac cacaaacccc gcatcggtaa gccatccgtt gcgtttacat
    16741 gggacagagt aaaaaattga ataaacaacc gagttcgctt tcaccgctgg tgcaattggc
    16801 gggaattcgc aaatgctttg atggtaaaga ggtcattccc cagctggatc tgactatcaa
    16861 caatggcgag ttcctcacgc tgcttggccc ttctggctgc ggtaaaacaa ccgttcttcg
    16921 cctgattgca ggtctggaaa ctgttgattc cggacgcatc atgctggata acgaggacat
    16981 cacccacgtt ccggcggaaa accgctatgt gaacactgtt ttccaaagct acgcactttt
    17041 cccccacatg accgtgttcg aaaatgtggc ctttgggttg cgcatgcaaa aaacccccgc
    17101 agctgaaatt acgccccgcg tgatggaagc cctgcggatg gtgcagctgg aaaccttcgc
    17161 tcaacgcaaa ccgcatcagc tctctggtgg tcaacagcaa cgcgtcgcca ttgctcgcgc
    17221 ggtggttaac aagcctcgtc tgttgttgct ggatgagtcg ctctcagcgc tggattacaa
    17281 actgcgtaag caaatgcaga acgagctgaa agcgttacag cgtaagcttg gcattacatt
    17341 cgtgttcgta actcacgacc aggaagaagc acttaccatg tcggacagga tcgtggtgat
    17401 gcgcgatggt cgcattgagc aagacggcac gccgcgtgaa atctacgaag agccgaaaaa
    17461 cctgtttgtt gccgggttta ttggcgaaat caatatgttt aacgccactg tcatcgaacg
    17521 tctcgacgag cagcgcgtac gcgccaacgt tgaaggccgc gaatgtaata tctacgttaa
    17581 cttcgccgtt gaaccggggc aaaaactgca tgttctgctg cgcccggaag acttacgtgt
    17641 tgaagagatt aacgacgaca accacgccga agggctgatt ggttacgttc gcgagcgtaa
    17701 ctacaaaggc atgacgctgg agtcggttgt tgagctggaa aatggcaaga tggtgatggt
    17761 cagcgaattc ttcaatgaag acgatcctga ctttgaccac tctctcgacc aaaaaatggc
    17821 cattaattgg gtagaaagct gggaggtcgt actggctgat gaagaacaca agtaagttcc
    17881 agaatgtagt gattgtcact attgtcggtt ggcttgtgtt gtttgtcttt ctgcccaacc
    17941 tgatgatcat tggcaccagc tttttgaccc gcgacgacgc cagtttcgtc aaaatggtct
    18001 ttacgctgga taactacacg cgtctgctcg atccgctcta ttttgaagtg ctattgcact
    18061 cgctgaatat ggcgctgatc gccaccctcg cctgcctggt gctgggctat ccttttgcct
    18121 ggtttctggc gaagctgcca cacaaggtgc gtccgctgct gctgtttctg ctgattgttc
    18181 cgttctggac caactcattg attcgtattt acgggctgaa aattttcctc agcaccaaag
    18241 gctatctcaa cgagtttttg ctctggctgg gcgttatcga tacaccaatt cgcatcatgt
    18301 tcacgcctag tgcggtgatt atcggtctgg tttacattct gctgccgttt atggtgatgc
    18361 cgctgtactc cagtatcgaa aaactggata agccgttgct tgaggcggcg cgcgatctcg
    18421 gtgccagcaa gttgcagacc tttattcgca tcattatccc actgacgatg ccgggaatta
    18481 ttgccggatg tctgctggtg atgctgccag cgatgggcct gttctatgtg tccgacctga
    18541 tgggcggtgc gaaaaacctg ctgatcggta acgtcatcaa ggtacagttc ctcaatattc
    18601 gtgactggcc gtttggtgca gccaccagca ttacgctgac tatcgtaatg ggcctgatgt
    18661 tgctggttta ctggcgcgct tctcgtttgc tgaataagaa ggtgagcgaa ttagatgatt
    18721 aatcctttat attcaatgca ttaagtttgt ttttgagtgg ttttgccgtt taaggtaacg
    18781 gcgttataga tcctatcaaa atcaatcaat tccattctgg ttttggagaa agatttttcg
    18841 agagcttcgg tctttaacta aaactgattg cgtataatcc caatacccct ccccgactat
    18901 ctgaatggtc ggggaggcac ttcaagcaca aaatatcatc cctgcgccgt tgattactct
    18961 gcaggtgaag tcggccactc aatatcaggt gcagttgatg tatcaacacg attcaacaat
    19021 acccgatatt tattccatgc ctccagcaac gatctttctt ccgccgttgc gatttccaga
    19081 tctacagcat cctgcagtgg cgcaatatac tcactgaatt cctggatgtg gaactgtgtg
    19141 gtgacggtct tccagtcatt cggctcctgc tgtatcgaag cataccaggc tatttcaata
    19201 tcgctatgct gcggcagcat ttaacccctt gtaattcatc gccataattg atttaattca
    19261 caaataaaac tataacatgg tgaaatcaat gaaaaaaaac acagatgatg gggctaaaat
    19321 ttacacacca cttaccctaa agctttatga ctggtgggtt ttgggagtat caaatcggct
    19381 tgcatgggga tgtcctacaa aggaacacct tcttccacac tttctggaac atgtaggtaa
    19441 caaccatctg gatattggtg ttggaactgg gttttacctt actcacgtac ctgagagtag
    19501 tctgatatct ttaatggatt tgaacgaagc tagcctgaac gcggcatcta caagggctgg
    19561 ggaatcaaaa attaaacata aaattagcca tgatgttttt gaaccttatc ccgcggcgtt
    19621 acatggtcaa tttgattcca tttccatgtt ttaccttctt cactgcctgc ctggaaatat
    19681 atctacaaaa agctgtgtaa tacgcaatgc ggcgcaggcc ttaactgacg atggaactct
    19741 atacggagcc acaattcttg gcgatggagt tgtgcacaat agcttcggtc aaaaactgat
    19801 gcgcatttac aatcagaaag gcatcttttc aaacacaaaa gattccgaag aaggcttaac
    19861 acatatactc tcagagcatt tcgagaatgt taaaaccaag gttcaaggta ctgtagtaat
    19921 gttttccgct tcagggaaaa aatagcatcc aaccgcagca cgttcttgct taagacgtgc
    19981 tgcggcataa tcccaatgat tactccctga cagggttcgt aggccactca atatcaggtg
    20041 cagttgatgt atcaacacgg ttcagcaaca cccgatactt tttccaggct tccagcaatg
    20101 aggtttcttc ctccgttgca atttccagat ctgcagcatc ctgaagcggc gcaatatgct
    20161 cactggctac ctgcatcagg ttgttttttg tttcttccgc ctcccggatc cggaacagtt
    20221 tttctgcttc cgtatccttc acccaggctg tgccgttcca cttctgaaac tccccttccg
    20281 gcgataacca ggtaacattt tccggtaacg gaccgagttc agaaataaat aactcgtccc
    20341 ctgacgctac gtcataaacc gttttccccc gatggtcttc aacgagatgc cacgatgcct
    20401 catcactgtt gaaaacagcc acaaagccag caggaatatc tggtggtgca atatcggtac
    20461 tgtttgctgg cagacctgta tgaggcggaa tatatgcgtc accttcacca ataaattcat
    20521 tagttccggc cagcagatta taaattttta tggtccgtgg ttgttcactc attctgaatg
    20581 ccattatgca agcctcacaa tatagttaaa tgcgatgttt ttgacggtgt tttccgcgtt
    20641 accagcagcg ttaacggtga tggtgtgtcc atgtgaacca atcgcaacgg agtgcgtatg
    20701 agcaccaata ccgacagtat gcgcgtgtgc acctgcagat gctgctgtgc cggacagtga
    20761 atggctatga ttaccatctg tactggtatt cgctaaccac cccgtagaca tacctactga
    20821 gccttgtaca ccccaggtat tttgacctga gcttgtataa ccatattgat aagtatcttt
    20881 aaaaacactg gggttaaatc gacggccatc tctatggctg tgattaccag ctgcattcgt
    20941 gctgccactt aaactatggg tatgcgcacc agtgttattc gtggatttag tgccgtaatc
    21001 aaacgacgat gtggttttcg tccccaaatc cgtactggat gcgctggcgc tgtgggtatg
    21061 cgatttaatg ccgtcctgtt cctgagacaa tacggcccga ccactggcag gtttgccctt
    21121 aatcgtccag ccacgcatat cagggatcac gcctgacgga taagcggctg caagtttcgg
    21181 gtatgcagat ttgtcaaaag tctgcccctg catcagggca tagccagacg gaacggtatc
    21241 tgatggccac gggattggtg cgccaggcgg ataaaactgc tctgatggcg tatagagtga
    21301 ataaactgta ccgtccgtta acccttccgg cttattagca gaatatgctg gtgacgtatg
    21361 aatcgtcacg ctggcattac tggtataatc ccattgaata tttacaccag tcgcataatt
    21421 tccgattgca acgtaaatat cgtaagtatc accagatgta ttgacccagg caaaatttgt
    21481 aaaccctgtc gatgtgcgct gccataaagc accagtaatc cccttcggat taccattacc
    21541 tgcacgtaaa acaagttcag atatacctgc ctgttgaggt gaccccacgt taaatccagc
    21601 gccaccaatc aacgtaattg aaacaacaga actcgcctgt ggcatggtta ccgttgctaa
    21661 tttgaaccaa ccagcaccac cgctgaatga cattgttgtt gagttaagcg taccaatatc
    21721 tttcggcgtc agtgttatat ccgctgaaag cgccttacca ttcaccttac gggcagaagg
    21781 tacccgacca ttcgcattgt cattagctgc tttcactgct ttcggtgtcg cagcaagcgt
    21841 ttcagatgtg ctgttggttg cactactgag ctggacaatt cctttttgtg ctgtcgtagc
    21901 gtcctgtgcg gtgtatttcc cgttagccag gtcatacgcg gccttaacgg cttttggcgt
    21961 tgccgcctgt gactcggaag tgctgttagt cgcactgctg agctgaacta tccctttctg
    22021 tgctgtcgtt gcatcctgta cggtgtattt cccgttagcc aggtcatacg cggccttaac
    22081 ggcttttggc gttgccgcct gtgactcgga agtgctgttg gtcgcactgc tgagctgtac
    22141 tatccccttt ttcgtcgtgc tcgcatcctc aagcgccacg gcggatgcaa tatcctctgc
    22201 ccgttttgct gctgtctcgg cgcgcgttgc cgcggattcc gccgtacttt tgctctgtgc
    22261 tgccgctgcc gcactgccag cagcctctgt cgccttcgtg gatgccgtcg tggcgctgcc
    22321 cctcgctgct gacgcctgtc tggtcgcctc atcttttgaa gcagacgcag atgatgccga
    22381 tgacgccgcc gaactggcgg acgatgcggc agccgttttt gaggattctg cgctggtttc
    22441 cgacgctttc gcgttcgttt cggatgtctt cgctgcggaa gcagacctcg ctgctgcgct
    22501 ggcctgttca gtggcttcgc cagccttcgt tgtggctgtt gaagcggatg atgcggcgct
    22561 ttctgccgat tttccggcgg cggtggcact gactgctgcc tcctctgcct taatggacgc
    22621 ctcacgggcc gatgatgcag ctgctgtctc agagtctgcc gcagctgagg cgctctgttc
    22681 cgctgccgtt tcagaagacc tggcattcgt ctcggacgtt tttgccgcct tcacggaatt
    22741 tcctgccgcc gttgccgagg aggctgcact gctggcgctt gtggaggcgt tcgtttctga
    22801 tgattttgcc gcctctttgg aggccgccgc atctctggct gaagtggcgg cttctgacgc
    22861 tttcgtcgtc gcagtggatg cagaagtggc tgctgatttt tgtgacgctg cagcattcgt
    22921 ttctgacgtt ttcgccgcac cggcactggt ggccgccgcg ctttttgagg actctgcagc
    22981 ggcagcactt tttgatgctt cagtggcctt tgttgatgcc gttcctgcgc tggaagacgc
    23041 ctcctgagcc gaagtcgcgg cctgtccggc tgacgtgctg gctgcacgcg ctgagcctgc
    23101 agcatcagtc gcatgggttg ccgcctcacg ggcagatgtg ccggcatcac tggctgactt
    23161 cttcgcggct gccgtgttct gtgccaccgc ggacgcgtta cgcgccacct cttccaccat
    23221 cagttcaaaa cggcgcagag cctccggacg ggcatcatcc tccgtcatgg caccgagaaa
    23281 atcattcagc gtaccgggtc gggaatcttc atacacggtg atggtcccgg catgtgacgg
    23341 cgggaatcct tccaccaaca gaataacgct gtactgaccg tactcaacgt ccatgctgta
    23401 acgcccggct tcatccggat tttctgaggc cagcgtgttc accaccaccg tggtgctgtt
    23461 acgttttgct ttcagctgga ttgtgcagtt ctgtaccggt tttcctgtgc cgtctttcag
    23521 tacacctgaa atctttactg ccatattcac cccacaaaaa agcccgcctg aaccggcggg
    23581 ctgtcataac actgtgttac ctggctaatc agaacttata accgacaccc acgatgaaac
    23641 cgtcagtgcg ccagtcgcca ctgccggagc cttcataagc aatatcaatg gccacggatt
    23701 cggtcgggtt aaactgcacg ccagctcccc acgccagaga cgtgttgctg tggcgaccgt
    23761 catcacttcc ggtcagcacg tcgtgcgttt tccccttgtt gtcagttacg cggagataat
    23821 ccccggagaa agtcgacaca cggctgtaag ccacacccgc catcgcatac gcgctgaacc
    23881 attcattcac gcgcacagac ggccccgcca ttacgctgaa ccagcggtta cgaacggaat
    23941 cttcatgcca gcgagtatcg ctgtaatggg tcagctggcg attcttgtct cctgcatagc
    24001 tgaatgacgt caccagcccc agcgtatccg taaactcata acggtatttc acgttaatcc
    24061 cgttcagatc atcactgccg ggaacgttgg tccgggcatg aagatacccc gcgctcagcg
    24121 tggactgatg ttcagacgcc catgcaggcg caccggatac ggccagacaa atggctgcgg
    24181 acaaaatggc ggcacaaact ttacgcataa ttacctctcg cttttctgca ataaaaaagg
    24241 cgtcattcct gacgccattt attggggtta taaatatttc aacgaatact gatgccggaa
    24301 gccgcttttt tggtcacaat caccgtacag tcggtgatat tgcctgcccc ctgattgcct
    24361 ttctggaaaa tcttaaactc cagagtgacg ctaccaccac cactaggcat atcaataact
    24421 gcactgtaac taccgggaat ggccccttta gtttctctgg atgcgattaa tacgccgttt
    24481 ttgcgaactt caaaaccata acccgtgtat cgcgtgcctc ccgggttatt tccgctcccc
    24541 ggatcgtcat acgctatacc gttaaaaata atgggcggaa taataatctg gcggtcaaag
    24601 ttatgatcat cgctgatggt gactgtaacc gtaccgtttg gtgtttccgt gttaccccac
    24661 gtaccgactt ttttcgggaa ggcttttgat acagctttaa cgaaatcccc tctgacctgg
    24721 gtcgcctcca gcatgccctt aatcgtacag ttctggttaa tcgtgacatt gttgagcgtt
    24781 cctgagttcg cattcacact accactgata tccgcatttt tagcggtcag ctttccgtct
    24841 gatgtcaggg aaaataccgg aggactgcca ccgctggtaa tggcgggggc tgtcaggcgc
    24901 ttcaggaaca cgtcgttcat gaatatctgg ttgccctgcg ccacaaacat cggcgtttca
    24961 ttcccgtttg ccgggtcaat aaacgcgata cggttagcgg caaccagaaa ctggctcagt
    25021 ttgccttcct ccgtgtcctc catgctgagg ccaatacccg cgacataatg tttgccgtct
    25081 ttggtctgct caattttgac gccccacatg gcattccatt tatcgttagc gtcctgccac
    25141 tctttcgaaa actcatccag tctgctggcg ttatcctccg tcagatcaac tttttccagc
    25201 agctccttgc cgagatggga ttcggttatc ttgcctttga aaaaatccag gtaaccttcc
    25261 gcatcatcgc tcgcccgacc gacggcctcc acgaatgccg atttgccaac ggtgttcaca
    25321 ctgcggatat aaaagtaata atcatggccc ggtttgatat tgatactggc ggctatccag
    25381 tacagcgccg taccaagata gcgggctgtg gtttctacct gcctgatatc ggtaatccgc
    25441 gtttccgaga accagaactc aaactgtacc gtcgggtcat aaacggcaag atgcggcgtg
    25501 gcggttatct gaaaatagcc cggcgtcagc tcaatccgcg acggcactgc aggtgcggca
    25561 atccggaacg ataccgatgc cggatcgccc tgctgccccc gggcatttac cgcccggact
    25621 gtcagcctgt agttccccag cgccagttgc gtgaagcggt atgtggtttc cgccgtccgg
    25681 gctgtgctga ccagccgctc actgccgtca tccgctgcca cggtcaggcg aagcatgaaa
    25741 ctcacgccct tcaccacctt cggcgtatcc cagcgggcca gtacctgata ctccccgctg
    25801 tctgcggtaa cttcggcagt caggtgctgc actgctggcg gcgtgacacc attcaccgtg
    25861 ccgctctggt cgccgtcaaa gtgcgccccg ttatccacga tggcttcttt ttccggtaca
    25921 tgctgcacgg cggtgatggc atacgtgccg tcgtcgttct cacggatact cacgcagcgg
    25981 aacaggcgct ggcgcagcgt cggcagcttc agcccccaca cgctgtatcc ggcaacgccg
    26041 tcaggaacac ggctcacttt taccttcacg ccgtcggtga cggactggac ctccacgctg
    26101 accggattgc cacttccgtc aaccaggctt atcagcgtgg taccggagga tggcagcgtg
    26161 atttcacggt cgagcgtcag cgtccggatc tggctgttta ccgccagcac gcgcccgccg
    26221 atgctgatac cggcatagtc atcatcacag atttcaatga catcgcccgg cacatggcga
    26281 agcccttctg cgcccacgct gaagtccacg gtctgcgttt ccagcagctc cgttttaatc
    26341 agccacagcc cggctcggtg tgcctgcccc cggctggtac agccaaaggc atccatcttc
    26401 gtgacattac gaccgtaacg ggcaatggcc tgcgtgtcct ccacaagctc tgttgccgtt
    26461 tcccagccgt tatccgggtc aatccagttc acctcaacgg cattatggcg gtccttcagg
    26521 gcgctgaaac tgtagcggaa cggcgcgcca tcatccagca tcaccacatt actgcggtta
    26581 taggtccaca ccttatccga cggccggtcc tgcacgaacg tcagcgtctg cccgttccat
    26641 accggcatac agcgcatcgc cgagcagaaa tcactgagca catcccacgc cttacgctgt
    26701 gtggtcaggt acgcattaca ggtgatgcgc ggctccgtac cgccaaaacc gtccggcacc
    26761 gactggtcgc agtactggcc gatgacatac agcgcccatt tatccacatc tgccgcacca
    26821 agacgtttcc ccatgccgta gcgcgggtgg gtcagcatat cccacagaca ccaggccatg
    26881 ttgttgctgt atgccggttt taacgttccg tcccagatac cgctgtattg ccgcgtctgc
    26941 gggttatagt tcgacggcac ctgcagaatg cgcccgtgaa gatgataatt acggctcacc
    27001 tgctggctgc cgaactgctc cgaatccacc tgtacgccga ccagtgccgt attcgggtag
    27061 cactgtttca catcgatgat ttcggtgtat gacgaccaga gcgttttgtt ctgcagctgg
    27121 tctgtggtgc tgtccggcgt catcctgcgc atccggatac tgaacgggcg cggcggcagg
    27181 ttatccacca ccaccgaggc cagatactgc gaggtggttt tgcccttaat ggtgatgtct
    27241 ttttccgtca cccagccacc attacgctgt atctgaacca gcaggcgaac ttccgacgga
    27301 ttccggtccc cctttgaggt ggtttccacc agtgcctgca caccgaaggt aaagcgcagt
    27361 cggtcaatgt ttgccgacgt gatggtccgg gtgatcggcg tgtcgtattt cacttccgta
    27421 cccagcaccg tctcggagcc ggaggattca aatccctccg gcggtgtctg ctcctgctca
    27481 ccggcccgga acaccaccgt gacgccggag atgttggtat tcccctcact gtccagcact
    27541 ggcgtactgt tcagcagcac gctttttaat ccatccaccg gaccttcaac cggcccttcg
    27601 ctgatggcat cgatcacact cagcaactgc gtggacttca ggttgtcctt cgtttcgcgc
    27661 ggagtatgcc ccttactgct gcctttaccc attcctaacg ctccataaac gacaaaaccg
    27721 cccggaagcg gtttcacata aaacgttttt catcaacgac caatcaccac aacctgacca
    27781 ccgtcccctt cgtctgccgt gctgatctcc tgagagacca cccgcgaccc cacgcgcatt
    27841 tcaccgtaca gaacgggcag aacattgccc tgggcaacca tgttatccag tgaggagaaa
    27901 taggtgttct gtttgccgtt atctgtactt gctgccgtgg gcgtcctggc tttcggtgcc
    27961 agcatctgcg ccactccgcc caggatcata ctggcccctg ccgcatacat gcccgataca
    28021 gccgcggcac ccagccagcc cacagggttc caccatgcca ccgcaatcag cgccgccccc
    28081 agcaccacct gaaacacacc gccactttta gctcccgcca gacgcggcac gatgtggatc
    28141 acggcaccat ttgccagcgg ctcattcaga cgggcagaca attcggtttc acctgcatca
    28201 cgcccggcaa tgcgtacctg gtaccagccg tcgctcagtt tctgacgaaa cgccgggatc
    28261 tgcatggcca gcgcccggat ggcttcggac cccgttttca ctcgaaggtc gatgcggcgg
    28321 ccaaatcgtt gtaaatcccc gtaaaggcag atgcgtgcca tgcccagtga cgccagaggg
    28381 agtgtgtgcg tcgctgccat ttgtcggtgt acctctctcg tttgctcagt tgttcaggaa
    28441 tatggtgcag cagctcgctg tcaccacagt aaatggcggc atgattcggc accgatgaac
    28501 caaaacagca cagcagcaca tcgcccggct gcgccgctga caacggcacc tgatacagcc
    28561 ctgtggcctc cagattatcc agatagagat tctgaccgtg acgccaccag tcatcctcgc
    28621 gatgaaaatc cggcatctca atccccgcca gatggtaagc atcccggaac agcgtgtaac
    28681 agtccgtcac cccgtgctca aagcgccgcc cggaaagatg tggcacacag cggaacttgt
    28741 gaatcgcccc ccggcagacc agccaccacg gcaaatcact ctgcacctgc agccgccggt
    28801 cggcctcact cagccagggc agaccaccgg ggtggctgtg gaccagcgcc acaatctcac
    28861 cctgcatttc tgcctgcagc cagtcttccg gcgacatacg gaaatacgcc tccggctcac
    28921 cggagatatt cacgcagggg aaatatcttt ccccctccgg cgtgcttacc acgaagccgc
    28981 acgactccgc tggcgcacat cgccgggcgt gcgccagaat cgctgattct gtctgtgtca
    29041 tgggatttac tgcgaaagtt tgttaatgga aaggaagccg ccaaagttgc cgacgttatt
    29101 gcggaactta caaccgctca ggcatttgct gcatttatcc ttcgtgatat cggacgttgg
    29161 ctggtcatat tcatccgcga cagccggacc gctataaccg cactcgtcac cgcgataggt
    29221 ccaggtgcag gtgttggcca gcatgatacg tcccggaaaa acagcgccat ccgtttccgt
    29281 cggcgtggac agtacaaagg aggcactcac cgcgctcagt tcgctgcact gctcaatgcg
    29341 ccagcggctg atcacctcct gctccggatc ggcgtcactg tttccgttga cgaagttcac
    29401 cgcatccaga aaacgggcgt aaaccttacg ccggaccacc gttcctccga ccagactctg
    29461 caggtcttcc gccatcccgg tgaccatgcc gtgcaggtta gaaaccgtca gtgtcggacg
    29521 ggcagcactg cctttgccgt tcagttcaaa tcccgtcccc tgaatgggat acgcctgata
    29581 ctgtcgcccc tgccaggtga ccggctcacc tttatcgttc tgctcattac agaaaaaata
    29641 acgttctcca ccgaccactg tcagatcgat ttcccagagc accacctggg ctgactgagt
    29701 gaggcgtgtc gtctcatgat gtgtttcctg tggaatatcc tgcatcagag actcctatgc
    29761 cacgacctgt tcaaaatctg ccgttatggt tacccacaac gcccccacgc ttgccgacca
    29821 tttacgacaa accaccctga tcggcttcca gtcataaggt ggcgtccact gaaatgcgcg
    29881 gacgccaccg tgccgttcca gaaaggcttt taaagatggg tgttcacatt tacgaacacg
    29941 tatcgtcacg ctgtaaatcg acaactggtt attcagtccc gccgcacgac gctgttcata
    30001 accatcgccc agcttcactg tcaccacttt cggctctgat accacattca tatccgggcg
    30061 cactttccag tgaaacgtct ccattaccga tatgctccac ttaaccgacc accatcacgg
    30121 gcctgctgtt gcataaagtc cgctgccgct tttttcccaa ggtcataaac caccttcagg
    30181 gcagccggac ctatctgccc gttcgtgcca tcgttattga tctcgatgtt gtactgcggg
    30241 gcaaacatcg ccatacctga accaccaata tccgccacaa cccccagctt accgtcagca
    30301 ccacgacgca gtggcagaat ggcttcaggt ccagcttccc ccatcacacc cgcgcctttt
    30361 gcaaaagcaa aaaacgtcgg acggttaacc accgtgccac tgtaacgact caaatcagca
    30421 gactgataaa tccccccatt ggcattcagg gaaaggctgc tgagatcaaa tcccagtaca
    30481 ctgccaatcc cttttatcga tttcatcatg gttgcctgcg ccaggatttt cgccatatct
    30541 gacagcaccg aagaggtgaa ggatttgaag ttgagtttgc cggtagtgac aaaagttgcc
    30601 aggccatttc ccatgttgct gaaggctgac gagaacacct gttctgctgt tcctgccgta
    30661 ttatctgcat ccacggtaaa atcctgaaat gcacgcagga ctccgttttt ccagttaccc
    30721 tgcacaactt caagctgttg ccagtaacgg cgattctcat tcagttgtcg gttcaggctc
    30781 tccgtcagcg cctgctcggc ctttctgtag tcatccgtgt tatatgtccc tttctgctca
    30841 ctatcccgcc tcaactgctc cagctgttgc tggtatttct ggcgaagact cagttgtacc
    30901 tgatatcgct gccgctgctg atcacccata cccaccgtgg cgatatccag gtcatgttgc
    30961 tggcgctgag cgcgctcttc ttcagccagt tgactggtca gctgaattgt ttttttcttc
    31021 agatcgttga gtgccgtctg tttctgaagc tcctgctgtt ttacatccag cagcgtcagt
    31081 gcctgaatca gttcatcttt acgggccagc acactctttt catctgccgt cagttttttc
    31141 ccgtccagat cgctgatgcg ctgctgcaga gccagaagct gtttatgcgc ttctgtcatc
    31201 ttttcagtgg caatgcctgc tgactgtctt gcagcagcaa tctgtccttc cacctgtgcc
    31261 tgttgctgac tgtactgcag caataaccgg gtggcctcat cattacgggt ttcgcgtgtt
    31321 tttttcttac cggatgccag ggctttctcg taacgttcat tttcacgttg tatcgctgca
    31381 tccctgacag cctgatcggc gtactgcatg gaattaatac gcgcaatttc acgctgatgt
    31441 cgtgctgctt ccgtttcatt catccggttc agagcagcat tttcagcatt acgtcgtttc
    31501 tgttgctcct gataattccg ctctgcctgc tcttttgcat cctgcaaatc cttctggcgt
    31561 tttttctcct gaagatcgtt aagacgctgc tgatcgtatt caacctgaga agatgatgcc
    31621 gtccagggga gtcttttcgc ccgcgacact ttctcctgta aagcggcaat ctgttcatcc
    31681 agcgagtctt cacgaccaat attcatggcc gcatcccaga aacgactcca caaatcagac
    31741 agatacttca gcgtactgcc aagcgcattg aggttattat caatatccgc agtacgccga
    31801 ccggtttcct ctgccagtgc agacatggct atccgtgcag catcactgga ccgcccctgc
    31861 tccccaagga cgcgtatctg ctcaagctga gtggcagtaa gaaaatgcag ctcattgtcc
    31921 agagccttcg cggcatttac aggatcatcc ttcagccgct taaactgatt tatggtatcg
    31981 ctgaccgact ggccaaccga tcgctccatc tgtgcggcag ctctcgccac cataccgata
    32041 tcgtttccac gaaatgcacc actccccacc acctgagcca gcgcaccggc tgcagcatgt
    32101 tgcgtgatac cattcccgga aatagcacga ctgagcgtcc acagctgccc ggcagtgact
    32161 ccggcataat gccccgtcag cgacaactgg cggttaaatt cttccccctc cttctgaccg
    32221 tcataccagg ctttacccag accatagacg gccgcggcaa taccgccaat aaccccgcca
    32281 agcatcatgc ctttcggtga catcagtgtg tctatccatc cggcacggtt agccagcgtt
    32341 atcccggatc ccctcagcgc ccctaaattg ccgcgggcca gttcacctat cagaacgcct
    32401 atctcctggc gggctgctgc acttttcaga cccagcgaat gcgtggcttt tcctgcctgc
    32461 tccattttgc ggatatacac ttctgcagca ctgcttaccc ccagctgggc tgccttggca
    32521 cgaagcaact cagaagatga aagattctgg cgggttgcct gctctttaag ctgacggata
    32581 aacgccactt tctgtcgggt agcctcttcc tcagcctgtg taagaacacg ggttttcgca
    32641 gtaacctcag aaatcagcgc cagataatcc tgctgaccaa tcccgccact gtttctggcc
    32701 tgtcggatct gctgctgaat acgctgtaat tcctgcagcc ccgcactggc ctgtttcaca
    32761 ctgtcaatct gacgataaaa cgcagcagcc gctttatcct gagcctccgc cagagccatg
    32821 gcctgcgcct gttcctcgcg cattttctgg ctcagcgcct ccatgcgctg gcgggttttc
    32881 tccacctcgc gggccatgcg ttcatgagcc tgtgcgttct tctccaccgt ctgcgcatgg
    32941 acggatgcgg ctgttgcagc cgaagaagcc gcctgcgttg tctgccgggc ggcctgagtc
    33001 tgacgctcca taaaacgctg catacgggca gaagaccgtt ctgcatcgct ggctgcacca
    33061 ttcagaaggt ttttgatacg gggaatttca tttttaaact ctgccgcatc aatccccaaa
    33121 tcaatgacca ggttggctat ctggtccata acgcacacct ccggaaatac cttccccaag
    33181 atgcatcagt tcttcgtccg ttcgctccgg tatcccgttc tcttccggta aaaggctgaa
    33241 atcagccacc gcagcatcac tgctgccgga caccattctc acgatcaatg ccttcagcga
    33301 ggcaaactgc gcatccatcc acacatcact gaagctctgc atccggaaat aatcgcccca
    33361 ctcaccaagc tcagtggccg acatttccga cagcatccgc cgccagtctg cccgcctgaa
    33421 ctcccgggcg agccgcatga caaactgcat ttcccgcgtc aggacttttc cggcgtcagc
    33481 acctcatgct ccaaatcccc ggcattctca atggctccca taccgctcag cgacagaacc
    33541 atctccgccc ccgttcccag ggcatcatac gaccatgttg taataacgga tgcgcaaagc
    33601 gtctcaacat cctgagactg ttccgcattc cacagtgagc gggaaaccag ccaggcattg
    33661 atatccatcc ccatccgcag aaaagcaatc tgtcgttcag cctccggcag ttctccctct
    33721 tcggcatcaa actttgccgt tcgctgctga acaaacgcca gatattcaat tctctgcagc
    33781 ccggacagct cactgagcac cacggactgc ttttcataat taaacgtgcc ctgtttcaga
    33841 aacatcatgt tctccacctg caaaaaagcc ccggataacc ggggcaaatg atgagtatcg
    33901 tcctgttaac ctgcggcgct gacagccacc gtagccactg ccacaaaatc gccgtcagaa
    33961 gtcatgccca caatgctgac actgccctgc ttcacgcctt tcaccgtggc cacaagcccg
    34021 ttcagggtca ccgtggcagt ctgtggatct gtcgaatgca cactgatcgc tttgtcactg
    34081 gctccgtcag gttttactgt aaaggtcagc gtggtggttg ctcccacttt tacactggca
    34141 gatgccggtg ccaccgtcag cccggtaacg cccacggttt cagtgccttc ctctgccaga
    34201 tacggacgcc ccacaccgct gattttcact gtgcgggtca tcacgtcttt tgaagcaatg
    34261 gttttaccca gtgaactcag ccagccacgg aaaacatcaa cagtaccgtt gggatatttg
    34321 atacgaaacg cgcagacttc accggagtcg aacaactgaa ccagtttttt ctgcccgctg
    34381 tcccccggac gccaggccag cgtcgccgaa gtatcaccga ctgatttctg cccctgggtt
    34441 gtcgttttcc agtctgcatc ttcatcatcg agataagtgt catcttctgc atcagcggtc
    34501 atttcgccag gttgcagatc cttcaccatc gcaagacgca gccagtcagt gtccgataaa
    34561 gggttcacaa acgcatcgcc cttgccggta tacatccaga acgtcgttcc cgcacctttc
    34621 gtttttgcca gtggatttgg tgtggtcatt accacctcct taattcgtgt acgtgatctg
    34681 gtacgtgatt tccgccatcg cccaggtggc catctcatta tcacgttgat agttaaaacc
    34741 gagtgggatc agggtgtcga tgagtccgga aagtgctggt acatcattca gggccgggaa
    34801 aatggtgctc tccatccaca tatccagctc tgaatccggt gcctgtgccc ggatgaagac
    34861 agcaatatgc agaacagcct gccagtcatc ttcatccgtc atttttccgg tgtactgagc
    34921 atcactcagc cacaccgcca cggcaggcag ttcctgcgca tcaacaaatg ccggaagccc
    34981 gtcaaaaaac gtggcgctgt ctccacactg ttcccgaagg cgtgccagta cgacctggcg
    35041 gatttgtgta tgtcggttca tcgggtcagc cataacctca gttgttgttt cagtgcatac
    35101 cccagctgtt tcggcatttc cgcagcaatg atgtggtcgc gggcatcttc aaatgcctgt
    35161 gtcagcggtc cggacagcgg gattttcacc acatcaatgg ggtaacgatt tttgccatca
    35221 atacgtcgca tcacatgcca gcgaccattc gccagttgct gaataaacgc atcccggaaa
    35281 agatatttac ccaccttcag cacactgcca cggtactgca gttttccacc acgccgggcc
    35341 agtctgaccc gggctgtccc cagcttaatg gcgggcagat tgccccggtt aacgcggatc
    35401 ctggccgtca tttttcctga cggactggct ttaaacaccc ggacacgctg acgtaccagt
    35461 ttcaggggga tccctttcac ctggttatct cccgcaacgg tattcccggc aacctgccgg
    35521 gtggcaaccg agaccgcttt ctgtgccaca cggtttatcg cccatgcgct ggcctgtggc
    35581 accatacggg tatcaaggct gttcagattg cggatggcat tctcaagccc cttcatccca
    35641 cacctcatta ctcaataaag atcattggct taccgttaaa acgttcatgc cgtgtgaccg
    35701 tccattgttg tccgtcataa acaacgcgat ccccgcgccg tgggcggtat cccgaagaaa
    35761 acaccaccag agagaccgca ggtccggaca gagcattcag ctctgccagt gtttctcccg
    35821 ggatcacagt catatcgaca tcattaatcg aggctgtctt tcccatcttt ctgaccgtga
    35881 tcgcatccat acgcgctgcc agccgggaaa agggatcaga cattgagttt taccggcact
    35941 tcttctgcac tggttccggc atctgcccag acaaccccga ccagcggatc agagccgctg
    36001 ttagtcagct gaacttttcc tgacttcaga taaaccttct tacccgtttt catgtcatcc
    36061 gttttcagct taggcagcat aaacacacct tcggttatgc cgtcgcctgt ttctccctgt
    36121 ggaatatcgg tcagcgccac cgcaaaaaca tcacccacct gcaccagatc tccgctgctg
    36181 atggctgcac ttgcaacaat cgccaccgtt tttccttctt ctacaaaatt ctttgccata
    36241 actgtctccg cacagccccg ttcaggggct gatttcaggt acaaaaaaag cccttacggg
    36301 ccatcagagt tgttgtctgc gacgtttacg ccgtacattt caccaggccg cggtgatcaa
    36361 ctggcgcaac accagcgtca atacgcactt tcgttgtcac gccatccaca ctgaagccct
    36421 ccatctgatc aatatatggc gtatccacac cgttgagata agccacttca atcgtatcgg
    36481 agcctttgga cgcagccagg tagaaggtgg tctggctgtt atcatcaaga cgaggctctg
    36541 caataacggt cgcaaaatct ttcaccgggt taataatacc ggcgttaatg tcagccccct
    36601 tgacacttga ggagcgaatg acctggttag caacagactc catcgccgtc ggtaccagta
    36661 cgaacgcagg acgaatattc agatgacgct caccctcttt ctgaacgcgc atcaactggc
    36721 gggctttatc cagcgatgcc acgtccattg ccgcgctctc cagtacgttt gcatgtttcg
    36781 ctttatcgaa cagacttaca ttatctgtgg agattttcgg gttagacgtc agaatggcat
    36841 aaaccagatc ggcaatagtg gatttcgccg cacggcccag cttcatcggg acatcggtca
    36901 gcatattcag atcatcattg ataatggcct gacgggtgat actgaacagc tcgccatagg
    36961 tcgccagtgc aatagtggcc tgtttatctc cggtggtgac gtatttatat tctgcccctt
    37021 cacgcacctg acgcagagca ctgaagcccc ccatacccac acgatgggca attttaaaat
    37081 cagacaactg acctttccgc gtccactgtt cataggtttc aggggcatct tcccagccct
    37141 gcagaatggc tttgttcgca acatccagca gaatattacc gaagtcagac gtactgtgta
    37201 tgaacgccgc accgaccatc tgcatcgggt tataactgga aacaccaata ccacgttcag
    37261 tcagtgacat acgggcatat tcacgcaggg tcatcccgtt gtagacatta tcacgttcag
    37321 ttttttcaaa cccggcgcgc gccatcagcg cctggcggat cccgtccccc acaaaattac
    37381 cgttaccggc ataaatatga gccggggtat ttttattgga tggcgtggac tcgcgcccca
    37441 tctcgttcaa cagcttttcg cgggcctgct ccagcgaaca ttcaggatcg gcaagacact
    37501 gagcctgcaa cgactgataa cgcccgccaa acatggcaaa cagatcatta ataccgttta
    37561 cacgcgcttt ttgctctgcc aggacctgcg cacggatgct gttttcatcc accacgggtg
    37621 ctgctgcctg cactggcgtc cgggaggctg caggtccatc atcctgtacg cgtggtgcac
    37681 tgttgcgtgg cggagtaatc atatttcgaa tggattccgg catcttttta aattcctctg
    37741 tacgttttga ctgaatacat gccattgcct taacggctgg tgtcacctga tcagcaaatc
    37801 cctgtgccag acattcggca ccggacatcc aggtctcatc cgccagcatg gcagcaattt
    37861 catcggtggt tttcccggtt ttctgtgcat aagcgggtaa cagaaccgcc tcaactctat
    37921 cgagcaggtc ggcataggtg cgcatgtcct ccgcatcacc gcccgtaaag ccaaatggtt
    37981 tatgaatcat catgaaggtg ttttccggca taatgaccgg gtttcctacc atcgcaatga
    38041 ccgacgccat tgacgccgcc acaccgtcga cataaacggt aatggacgca ccatgtgttt
    38101 tcagcgcatt aaaaatggcg atgccttcaa agacatcgcc acccggtgaa ttaatatgga
    38161 gattaatgtg ggtgatatcg cccagtgcat tcagatcact gataaactgc ttcgctgtaa
    38221 caccccagaa accaatctcg tcataaatat aaatatccgc gtcactctgg tgaccagcct
    38281 gcatcctgaa ccaggaatta ttcttcggac tggtcgtcgg tgtgctgcgg ctcctgtcgt
    38341 ttcgttgcgg cactactgcc tcctttatca ctggccggat cggtatcaaa taccagatcc
    38401 agattgcggt tttcatcaat ttcggccttg cgccgacgtt tgacatcatc cggattacga
    38461 ccaccagcac gtacccagtc tgattctgtc gccgctccac cacgaatctg gattttccag
    38521 gcctcagcct ccttaacagg gtcaatccac ggcatcactg gtccggaata caccgcggta
    38581 tacagtgaag aacggtcaag atcgcggggt agcctgataa caccggatgc cacagcctgt
    38641 ttcagccatg cacgatacat cgggcgggtg acggcaccaa taaaccagtc ctgcaggatc
    38701 aggtagccat cagtggattc aaccagctcc tgacgctggg cgctgtaagt gccgttatag
    38761 ttgcgcgctg tactggaaaa actcagacga ctgcccgccg ccacggcacg caactgacca
    38821 ttacgaaaag tttcaaggtt aggattgggg cgatccgact tcaccattcc gatttcttcg
    38881 ccgggtttca gatcgtcgta aataatgccc ggctgaatgg taagctcacg ttcattctcc
    38941 tttctgccat taccatccgg ttcatagctc tgcccgtcgc ctttccggat gtacatcccc
    39001 agagcagcgg cgatccttgc tgcagtcagc tcagaatctt catattcttt cagggcactg
    39061 aggcggatca gcacaccgga caacaaagac gtcccgcgca tctggtgcag acggcgaaca
    39121 aatttaagat gcagcattcg ctctgcatcc acttctttgg tttccatctg ccgtccggat
    39181 acgggacggc ttttatacac cagatatttt tcgggacgcc cccagtcatc aacaaacacg
    39241 ccctgattca gcctgttgct ctcatcactg gtcatgggaa taaagtccgg ctcgagcgcc
    39301 tccagccaga aatgaacacc ggcagaaggc gccaggctgt ttatgcgccc ggaaaccatc
    39361 tgggcaaaca cctcaccatc gcgcagccag gtacgaagca tcagacgttc cagcatcgga
    39421 cgggtaaact gcccggtgac ttccgggctg acagaccatt cactccatcg ggtgcgaatc
    39481 tccgcagcca ggtcacgggc aatggcccca ttgcgtaata ccggatgtgg ctcgacaata
    39541 atcccgtttt tccccaccac ccgttcttcc agcttgtcaa atacaccaat aaccagatcg
    39601 tggttgttat caaggtaacg ggcctgctca cgtaacgaca cggccccgta ctggcttaac
    39661 tggtcggcag ttcggttctc ccgtcgggct ttgtgtgtcc gcgtcgtttt tacggcctca
    39721 taagcctgga tcaccgcacg ggaacgcagc cttgccgctt tccatcctgg tgaaaaaacg
    39781 ccaatcacat catcaagaat tgccatcaga acctcgccag ccggtacccg ggatgccccc
    39841 gtcgtcgtgt aatcagagcc gcaaggcggc gctcccactc ctgccgtccc tgccggatct
    39901 cagataagtt ttccatggtc atctgctgac cattaaaggt gacggatttt ccgtccagca
    39961 ccgccatttc agcttccgta taacgctgaa tcatggcttc gatatcattc tggttcataa
    40021 ccatcctccg gaagtcagcc aggggttaac atcgtcagtt actgttttct tccgtttttg
    40081 ttttttaaca ggcgtggata ccggttccgg tgaggatgac ggttcggtac tgtcctggac
    40141 acactccagc caggtctccc ggctcgccca ctccggtgca tccggccagc ggatcttttc
    40201 gtatccatgc agaatgacca gagcctcggc atacaccatc aggtcaaaag cttcgttggc
    40261 accgcgaccc ggcttactcc atttcccgtc actgctccgc tcttcatacg tcagttcgtc
    40321 gtaaaaccag ctccccagcc agtcagggaa atgcacatag cctggacctg gcgagtcacg
    40381 ccataacgcg ttattcaccc ggtctttcag ggcatccgtc tgaagaagcc agagcggcac
    40441 atcacctgcg gcctgcgccc ggcggcccgt tcgtccggtg ttatcaggga atgtacggct
    40501 gatcagtttt gcgcgccgga tgctgtcacc cttaaacagg taaatacgtt taccaaggcc
    40561 atcacgacgg caacgacgcc agaatttata ggcattatca gtgaccccgt cttcaccgcc
    40621 ggagtccacc gccattgcca tcagtcgcat ttgttgagaa ggatcggagg ccagcggcca
    40681 gcttttatga aaaacatccg tcagcaggac atcccagtct tccggatagc tggccggatc
    40741 aattcgctgg ctctccccgt cgcagtcact gcgcaatgac tgcgtgatgt tgtaacgatc
    40801 aataatccag cgttcgccac gactgccata gcccgttacc tgaaccacaa aacggcgatg
    40861 acgtcccgcc tgcacatcca ctgtcgccac aaggaaatta acgccatccg gcacactgcg
    40921 ggaaggaact ggctctgccc gctgctcaag cagttcactt tttcgttgct ccatgctggc
    40981 acgaggaaga taaggcaatc cccagtcggt gttgataacc gccctgagtg tttcttcgct
    41041 tcctgtcgct tcatactcct gttctgcagt cagtaatttg taaaccagtt gcgcccaggt
    41101 ctgatacgca gcagctggcc cttccatcca gaaactggcg atacgggagc ggcgcggttc
    41161 accggaaacg ttgccgttac gatcaatgac ctgaccttca cgcaaccaga ctcctgcact
    41221 attgagctca cgctttttct ccgcagtgat aatgccgctg cagtgcgggc aaagtagata
    41281 cgccgcctca ctggctttaa agggatccgg ttcattacgg tagccggtca tggcatccat
    41341 ggctggctga aaatattcac cgcagtgcgg acatgaccag taccagcggc ggcggtcacc
    41401 acgattgtaa agggaaagaa taccagtcgt cggtggcgct tcatgaggcg acttacgtcg
    41461 ccatttgctg tcgcagatgt cacgtcccgg cgagctctcc accagagtca tcccggcgga
    41521 cataaatgtg gtggtacgtt ttgaggccag ggagaaacca tcaccctcgc tgtcgatatt
    41581 ctccggaaaa cggtcgtaat cggttaaggc gacaaaccgg taatccgacg acgacataat
    41641 gttgaccgag ggccaaccaa ttttaaggaa cgagccatcc ctgaacgtct tatcatggac
    41701 attattgtcg ttacgacgtg gactcattct tttctttacc gccgcactgc ttctgaacgt
    41761 tctgtcgagg cgctttttag aatgctcgcg ggccttatct tcggtcatct gcacaacgag
    41821 catgtccgaa ggatcgcaaa cgatggtata gacaatccat ccatcgatca gaccaatggt
    41881 cttccctgtt cgcgcaggac caacaaaaat caccgcatcg tattcacgcg atgccaggca
    41941 gttcatgggc tcaatgatgt agggtgtcag ttcaggatcc catggcaccg agttaccagc
    42001 ccccttggga acacgcatga attttttaac agcctccgaa atcggcatgc gacgtggtgg
    42061 ggaaaatcct gccgatatgt cccttcccaa atttcgggct gatgaaaaac ccattattcc
    42121 tcctagagac tctctccttc ctcatcagga attaattcag cagcacaagc ctcgtaggat
    42181 ttttcctgaa gagtgtatcg caggtcatca atggcctgct gtacaacgcc gacggcctga
    42241 ggagtcaggg cgcaatcgcg ttcaagaaca tccggaattg tctccagaac ctggacgaca
    42301 gcctttctca tggacgaata gacgatgact acttcatcaa cggggatgag ttttcgctgc
    42361 tccttttcca gcttgatcct ttcattttca gattggtacc agtcctttct ctctttcggc
    42421 tccatacggg atggatcatg aacagagtct gctgcctcat gcttcacact aaacagagct
    42481 ggcccgacat gctgcagggc gtaaacgggg ttccccctga tagtcgcagc cacaggagtg
    42541 ttggccgcga ggagccgttt ttttactgtg tcccggtgaa gcccaaaggc ctcagcgatt
    42601 ttaaaaacac tccagtaata agcatcaccg atcccgctca catttgacat aagcaactcc
    42661 atctggcagg tgaaaatcat atttatttat atatttcaat taattgcaaa ctgatctaat
    42721 gacagggaga aaaaaatatt gtacaggtga aaagagaaat aacttttaat tatcaataaa
    42781 ttaccgaaca tgctgccgcc gccatggaaa tgcaaaaact agcctttttc cgcgacgctc
    42841 ccgccccgtg gcaggggccc ccaccgggag gacccgacag cctgacagcc tgacagccgt
    42901 gatgagcatc tgatacagcg ctttacataa tggcatagga ataattcaga aggacatcac
    42961 agcatgccca cacaaattag tgtgagtgtc ctgtttcttc ctacgcacag gactgacgag
    43021 catgagggga aaatatgcga accataaatg cctcatcttc agcaatgcaa ccggcatccg
    43081 aacaggacga tattgtaaat tcacctaatt acgaggacat tgcagtagtt gaattgcagc
    43141 tctgtattag cctgacagtg acagaatgcg atattgactc tgtcacaggt gaaatagttt
    43201 gaatgattag cagttatggt gctcagtcaa ccaccaggga ataatccttc gaattcttat
    43261 cgtgcttcac caacgctgcc tcaattgccc tgaatgcttc cagagacacc tgatgttcta
    43321 tacatgcaat tacaacatcc gggtaactca tagaaatggt gctattaagc atatttttta
    43381 cacgaatcag atccaacgag agttcatcag cagattgttc tttattcatt ttgtcgctcc
    43441 atgcgtttgc tcgtcatcta gcggttaaaa tattacttca aatcttactg cataaaagag
    43501 gaaaagtatg aaagtttgag tacatcgacc ttacatacat ctgtcggttg catatccctc
    43561 ctggatgcca gcaaggctca attttgttac gcaatcaaca ctattcatca aaaacaggct
    43621 taatatttga cataaatcat caacaaaaca caaagaggtc agaccagatt aaaacaataa
    43681 acacgataat gctaactacg cgccatcgta tcacatggaa ggtttaccaa tggctcaggc
    43741 tgccattttt aaagaaatat tcgatcaagt gcgtaaagat ttaaactgtg aattatttta
    43801 ttctgagcta aaacgtcaca atgtctcact ttatatttac tatttagcca cagataatat
    43861 tcacattgtg ttagaaaacg acaacatagt gttagtaaaa ggactcaaaa aggttgtaaa
    43921 tgttaaattc tccagaaaca aacatcttat agagacctcc tataataagc tgaaatcaaa
    43981 agaaatcaca tttcaacaat acagggaaaa tcttgctaaa gcaggagttt tccgatgggt
    44041 tacaaatatc caggaacacc aaagatatta ctatgccttt gataactcat tactatttac
    44101 tgaaagcatc cagaaaacta cacagatctt accacgctaa accataacgt ccggcttctc
    44161 tcactcctga gccggactgc attggtttaa taaaaaccat caacaattgt gatttagata
    44221 ttcggaacca ttcaaatata acaaaacccc gtaaaaacga ggtttatgga taaattttat
    44281 tattgaatac atcagattaa attaatcttg acatcatagc tttcaagacc cgtcattttt
    44341 tcccgtgcgg taaactgaat actggtaact tctttcccgg tctttttctt aagttcaata
    44401 attttttttg ttatatattc agaaatatct gcttctgctt ttgtttttaa gttttcaata
    44461 ttcatcattt cctcttttag tctgttatga ctttccagtt acacagtaag tcgattatat
    44521 ggtgcaaacg tgtaaaagat aagatgaaac atcgcaataa tcaacatacg atagtctaaa
    44581 ttttacacaa acagacaaag agaattttcc tgaattatca atacaatagc atcaaatcaa
    44641 ctcaagagcc ttattgctgc ttccagaatt tcttctgaag taacatgtcg atccgcggct
    44701 acataaatga ctttatgatc tccggtcaga gatggaaacc ctgcggccat tacagtaagg
    44761 tgtgtttttt cgccatttgg atattcacgc atgatggtgt taactccagt catcgctggc
    44821 actaccactg ctggttcaga gttaaaaaaa actatgattt ttttcatgat gttaccgtag
    44881 tatgtgagta tccatcgaat agataccaag caaaaaagct cccgaaggag ccttcatttt
    44941 cactttttta aatccaacga cagacggctg gcatttaagt attgtgaaat attatcaaat
    45001 gtaatcatca ttgatttaca aaagatacat tttgccccga aaggattcat gtcagaaaca
    45061 t
//
LOCUS       DAHHTG010000023        43845 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.023, whole genome shotgun sequence.
ACCESSION   DAHHTG010000023 DAHHTG010000000
VERSION     DAHHTG010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 43845)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43845)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43845
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.023"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            complement(28..864)
                     /gene="fdhD"
                     /locus_tag="NBI74_001284"
     CDS             complement(28..864)
                     /gene="fdhD"
                     /locus_tag="NBI74_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000753607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HCD1931648.1"
                     /translation="MKKTQQKEIENVTNITGVRQIELWRRDDLQHPRLDEVAEEVPVA
                     LVYNGISHVVMMASPKDLEYFALGFSLSEGIIESPRDIFGMDVVPSCNGLEVQIELSS
                     RRFMGLKERRRALAGRTGCGVCGVEQLNDIGKPVQPLPFTQTFDLNKLDDALRHLNDF
                     QPVGQLTGCTHAAAWMLPSGELVGGHEDVGRHVALDKLLGRRSQEGESWQQGAVLVSS
                     RASYEMVQKSAMCGVEILFAVSAATTLAVEVAERCNLTLVGFCKPGRATVYTHPQRLV
                     HN"
     gene            1058..4108
                     /gene="fdnG"
                     /locus_tag="NBI74_001285"
     CDS             1058..4108
                     /gene="fdnG"
                     /locus_tag="NBI74_001285"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:1643..1645,aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HCD1931649.1"
                     /translation="MQVSRRQFFKICAGGMAGTTAAALGFAPSVALAETRQYKLLRTR
                     ETRNTCTYCSVGCGLLMYSLGDGAKNAKASIFHIEGDPDHPVNRGALCPKGAGLVDFI
                     HSESRLKFPEYRAPGSDKWQQISWEEAFDRIAKLMKEDRDANYIAQNAEGVTVNRWLS
                     TGMLCASASSNETGYLTQKFSRALGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANLVVVMGGNAAEAHPVGFRWAMEAKIHNGAKLIVIDPRFTRTAAVADYYAPIR
                     SGTDIAFLSGVLLYLLNNEKFNREYTEAYTNASLIVREDYGFEDGLFTGYDAEKRKYD
                     KSTWTYELDENGFAKRDTTLQHPRCVWNLLKQHVSRYTPDVVENICGTPKDAFLKVCE
                     YIAETSAHDKTASFLYALGWTQHSIGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSQSLPGYMTLPSEKQTDLQTYLTANTPKPLLEGQVNYWGNYPKFFVSM
                     MKAFFGDKATAENSWGFDWLPKWDKGYDVLQYFEMMKEGKVNGYICQGFNPVASFPNK
                     NKVIGCLSKLKFLVTIDPLNTETSNFWQNHGELNEVDSSKIQTEVFRLPSTCFAEENG
                     SIVNSGRWLQWHWKGADAPGIALTDGEILSGIFLRLRKMYAEQGGANPDQVLNMTWNY
                     AIPHEPSSEEVAMESNGKALADITDPATGAVIVKKGQQLSSFAQLRDDGTTSCGCWIF
                     AGSWTPEGNQMARRDNADPSGLGNTLGWAWAWPLNRRILYNRASADPQGNPWDPKRQL
                     LKWDGTKWTGWDIPDYSAAPPGSGVGPFIMQQEGMGRLFALDKMAEGPFPEHYEPFET
                     PLGTNPLHPNVISNPAARIFKDDAEALGKADKFPYVGTTYRLTEHFHYWTKHALLNAI
                     LQPEQFVEIGESLANKLGIAQGDTVKVSSNRGYIKAKAVVTKRIRTLKANGKDIDTIG
                     IPIHWGYEGVAKKGFIANTLTPFVGDANTQTPEFKSFLVNVEKV"
     gene            4121..5023
                     /gene="fdoH"
                     /locus_tag="NBI74_001286"
     CDS             4121..5023
                     /gene="fdoH"
                     /locus_tag="NBI74_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase O subunit beta"
                     /protein_id="HCD1931650.1"
                     /translation="MAYQSQDIIRRSATNGLTPAPQARDFQEEVAKLIDVTTCIGCKA
                     CQVACSEWNDIRDTVGNNIGVYDNPNDLSAKSWTVMRFSEVEQNDKLEWLIRKDGCMH
                     CSDPGCLKACPAEGAIIQYANGIVDFQSEQCIGCGYCIAGCPFNVPRLNPEDNRVYKC
                     TLCVDRVVVGQEPACVKTCPTGAIHFGTKESMKTLASERVAELKTRGYDNAGLYDPAG
                     VGGTHVMYVLHHADKPNLYHGLPENPEISETVKFWKGIWKPLAAVGFAATFAASIFHY
                     VGVGPNRADEEENNLHEEKDEERK"
     gene            5020..5655
                     /gene="fdoI"
                     /locus_tag="NBI74_001287"
     CDS             5020..5655
                     /gene="fdoI"
                     /locus_tag="NBI74_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HCD1931651.1"
                     /translation="MKRRDTIVRYTAPERINHWITAFCFILAAVSGLGFLFPSFNWLM
                     QIMGTPQLARILHPFVGVVMFASFIIMFFRYWHHNLINRDDIFWAKNIRKIVVNEEVG
                     DTGRYNFGQKCVFWAAIIFLVLLLVSGVIIWRPYFAPAFSIPVIRFALMLHSFAAVAL
                     IVVIMVHIYAALWVKGTITAMVEGWVTSAWAKKHHPRWYREVRKTTEKKAE"
     gene            5652..6581
                     /gene="fdhE"
                     /locus_tag="NBI74_001288"
     CDS             5652..6581
                     /gene="fdhE"
                     /locus_tag="NBI74_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory protein FdhE"
                     /protein_id="HCD1931652.1"
                     /translation="MSIRIIPQDELGSSEKRTADMIPPLLFPRLKNLYNRRAERLREL
                     AEHNPLGDYLRFAALIAHAQEVVLYDHPLEMDLTTRIKEASAQGKPPLDIHVLPRDKH
                     WQKLLMALIAELKPEMSGPALAVIENLEKASTQELEDMASALFASDFSSVSSDKAPFI
                     WAALSLYWAQMANLIPGKARAEYGEQRQYCPVCGSMPVSSMVQIGTTQGLRYLHCNLC
                     ETEWHVVRVKCSNCEQSGKLHYWSLDDEQAAIKAESCDDCGTYLKILYQEKEPKVEAV
                     ADDLASLVLDARMEQEGYARSSINPFLFPGEGE"
     gene            complement(6763..7005)
                     /locus_tag="NBI74_001289"
     CDS             complement(6763..7005)
                     /locus_tag="NBI74_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1931653.1"
                     /translation="MNPLAGMDMSRILLDLSNEVIKQLDDLEVQRNLPRADLLREAVD
                     QYLVNQSQTARTSVPGIWQGCEEDGVEYQRKLREKW"
     gene            7295..8143
                     /locus_tag="NBI74_001290"
     CDS             7295..8143
                     /locus_tag="NBI74_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001038650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1931654.1"
                     /translation="MNKNESISYTEMQKFFAREIKEVGGRPITSKNLELSLLPIHARE
                     NLSAPEMPWYTEVNDIYMEFRRSPFWMVAGYVFLGFMLSLLSISILSIVAFSFYEMGF
                     NSDLLFLYLLAFVLGCFILPGAAKIAFFTPHYLPIRFNRKTQKIYVSDLIIEGNVFFG
                     KIKLTFHEYDWKDVEGWEVSRHFSKSMPYNGLHLVVNRENDINALRQTRVYTTRAPWS
                     AQEMARQKNYIPQIWLYCHNYMAYKTVSGAKNKPQPFIFLRNNWLFKWPKEMDKRSRS
                     SKILSE"
     gene            8459..8908
                     /locus_tag="NBI74_001291"
     CDS             8459..8908
                     /locus_tag="NBI74_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001523573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1931655.1"
                     /translation="MKDNIHTAVIMAGEFLSANEWIRRKNQLKTSVHPASVASFAPYG
                     FNINQCDDDGNLWTNFPRPHIKLKIAPGKTACFLCRREELLFGYDEAHNHYQPNLNLV
                     LFVKNHGLHKDVYLKSHDDKSEYDSRILCNEKFTTYDFRKNRSRYSF"
     gene            complement(9093..9311)
                     /locus_tag="NBI74_001292"
     CDS             complement(9093..9311)
                     /locus_tag="NBI74_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312842.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix protein, CopG family"
                     /protein_id="HCD1931656.1"
                     /translation="MAMNTVFLHLSEEAIKRLNKLRGWRKVSRSAILREAVEQYLERQ
                     QFPVRKAKSDRQRGEVVGVEEQGKWHEE"
     gene            complement(9708..9986)
                     /locus_tag="NBI74_001293"
     CDS             complement(9708..9986)
                     /locus_tag="NBI74_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1931657.1"
                     /translation="MSTFNTRKDRDRNGKAKPHKHSGIPTLTGKWRHPSMDCWVNYST
                     PGDHEWKRLYTTLRRRADDRRQCHRVMQGDDPEGIVWFPDCYPQIYYY"
     gene            complement(10345..10635)
                     /locus_tag="NBI74_001294"
     CDS             complement(10345..10635)
                     /locus_tag="NBI74_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000356400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCD1931658.1"
                     /translation="MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRAS
                     GLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALLRAIANDPQHVLQALNR"
     gene            complement(10636..10948)
                     /locus_tag="NBI74_001295"
                     /pseudo
     CDS             complement(10636..10948)
                     /locus_tag="NBI74_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000897300.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            11177..12085
                     /locus_tag="NBI74_001296"
     CDS             11177..12085
                     /locus_tag="NBI74_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCD1931659.1"
                     /translation="MALEKGIAELVEEFIAAGRPSSRQQSITQRREGYIASAVLAGET
                     ETRVDIQTIELEGMTLRIVSPLNAPTLLPTIIYYYGGCFVSGGFATHDNQLRQLAYYG
                     QCRIIAVQYRLAPEHTFPAAHDDAQRGAELVRQHAERLGVDKQRITLAGDSAGGHLAL
                     VTALRLKRAGEWQPAQLILIYPMLDATAHFESYIRNGHDYIITRDTLLSGFEMYLPGI
                     ERRHPEASPIWRNDFNGLPPVHIITAEYDPLCDEGEALYHRMTGQGVQCTCQRYLGVI
                     HGFFQLGGISEAARSALRDVAWRAGR"
     gene            complement(12149..13090)
                     /gene="fabY"
                     /locus_tag="NBI74_001297"
                     /gene_synonym="yiiD"
     CDS             complement(12149..13090)
                     /gene="fabY"
                     /locus_tag="NBI74_001297"
                     /gene_synonym="yiiD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid biosynthesis protein FabY"
                     /protein_id="HCD1931660.1"
                     /translation="MYHLRVPQTEEELERYYQFRWEMLRKPLHQPKGSERDAWDAMAH
                     HQMVVDEQGNLVAVGRLYINADNEASIRFMAVHPDVQDKGLGTLMAMTLESVARQEGV
                     KRVTCSAREDAVEFFAKLGFVNQGEITTPTTTPIRHFLMIKPVATLDDILHRGDWCAQ
                     LQQAWYEHIPLSEKMGVRIQQYTGQKFITTMPETGNQNPHHTLFAGSLFSLATLTGWG
                     LIWLMLRERHLGGTIILADAHIRYSKPISGKPHAVADLGALSGDLDRLARGRKARVQM
                     QVEIFGDETPGAVFEGTYIVLPAKPFGPYEEGGNEEE"
     gene            complement(13135..13572)
                     /gene="dtd"
                     /locus_tag="NBI74_001298"
     CDS             complement(13135..13572)
                     /gene="dtd"
                     /locus_tag="NBI74_001298"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000560980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HCD1931661.1"
                     /translation="MIALIQRVTRASVTVEGEVTGEIGAGLLVLLGVEKDDDEQKANR
                     LCERVLGYRIFSDAEGKMNLNVQQAGGSVLVVSQFTLAADTERGMRPSFSKGASPDRA
                     EALYDYFVERCRQQDMNTQTGRFAADMQVSLVNDGPVTFWLQV"
     gene            complement(13569..14441)
                     /locus_tag="NBI74_001299"
     CDS             complement(13569..14441)
                     /locus_tag="NBI74_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor BrkB family protein"
                     /protein_id="HCD1931662.1"
                     /translation="MLKTIQDKARHRTRPLWAWLKLLWQRIDEDNMTTLAGNLAYVSL
                     LSLVPLVAVVFALFAAFPMFSDVSIQLRHFIFANFLPATGDVIQRYIEQFVANSNKMT
                     AVGACGLIVTALLLMYSIDSALNTIWRSKRARPKIYSFAVYWMILTLGPLLAGASLAI
                     SSYLLSLRWASDLNTVIDNVLRIFPLLLSWISFWLLYSIVPTIRVPNRDAIVGAFVAA
                     LLFEAGKKGFALYITMFPSYQLIYGVLAVIPILFVWVYWTWCIVLLGAEITVTLGEYR
                     KLKQAAEQEEEDEP"
     gene            complement(14435..15034)
                     /gene="yihX"
                     /locus_tag="NBI74_001300"
     CDS             complement(14435..15034)
                     /gene="yihX"
                     /locus_tag="NBI74_001300"
                     /EC_number="3.1.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphatase"
                     /protein_id="HCD1931663.1"
                     /translation="MLYIFDLGNVIVDIDFNRVLGAWSDLTRVPLATLKKSFHMGEAF
                     HQHERGEISDEAFAEALCHEMALPLSYEQFSHGWQAVFVALRPEVIAIMHKLREQGHR
                     VVVLSNTNRLHTTFWPEEYPEIRDAADHIYLSQDLGMRKPEARIYQHVLQAEGFSPDD
                     TVFFDDNADNIEGANRLGITSILVKDKTTIPDYFAKVLC"
     gene            complement(15133..15918)
                     /locus_tag="NBI74_001301"
     CDS             complement(15133..15918)
                     /locus_tag="NBI74_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418320.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCD1931664.1"
                     /translation="MSLTELTGNPRHDQLLMLIAERGYMNIDELANLLDVSTQTVRRD
                     IRKLSEQGLITRHHGGAGRASSVVNTAFEQREVSQTEEKKAIAEAVADYIPDGSTIFI
                     TIGTTVEHVARALLNHNHLRIITNSLRVAHILYHNPHFEVMVPGGTLRSHNSGIIGPS
                     AASFVADFRADYLVTSVGAIESDGALMEFDVNEANVVKTMMAHARNILLVADHTKYHA
                     SAAVEIGNVAQVTALFTDELPPAALKSRLQDSQIEIILPQEDA"
     gene            complement(15952..16848)
                     /gene="yihV"
                     /locus_tag="NBI74_001302"
     CDS             complement(15952..16848)
                     /gene="yihV"
                     /locus_tag="NBI74_001302"
                     /EC_number="2.7.1.184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418319.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfofructose kinase"
                     /protein_id="HCD1931665.1"
                     /translation="MIRVACVGITVMDRIYYVEGLPTESGKYVAKNYTEVGGGPAATA
                     AVAAARLGAQVDFIGRVGDDDTGNSLLAELESWGVNTRYTKRYNQAKSSQSAIMVDAK
                     GERIIINYPSPDLLPDAEWLEEIDFSQWDVVLADVRWHDGAKKAFTLARQAGVMTVLD
                     GDITPQDISELVALSDHAAFSEPGLARLTGVKEMVSALKQAQMLTNGHVYVTQGSAGC
                     DWLENGGRQHQPAFKVDVVDTTGAGDVFHGALAVALATGGDLAESVRFASGVAALKCT
                     RPGGRAGIPDCDQTRSFLSLFV"
     gene            17016..17912
                     /gene="yihU"
                     /locus_tag="NBI74_001303"
     CDS             17016..17912
                     /gene="yihU"
                     /locus_tag="NBI74_001303"
                     /EC_number="1.1.1.373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfolactaldehyde 3-reductase"
                     /protein_id="HCD1931666.1"
                     /translation="MAAIAFIGLGQMGSPMASNLLQQGHQLCVFDVNTEAVQHLVDKG
                     ATPAANPAQAAKGAEFIITMLPNGDLVRHVLFGENGVCESLSSDALVIDMSTIHPLQT
                     DKLIADMQARGFSMMDVPVGRTSANAITGTLLLLAGGTAEQVERATPILMAMGSELIN
                     AGGPGMGIRVKLINNYMSIALNALSAEAAVLCEALNLPFDVAVKVMSGTAAGKGHFTT
                     SWPNKVLSGDLSPAFMIDLAHKDLGIALDVANQLHVPMPLGAASREVYSQARAAGRGR
                     QDWSAILEQVRVSAGMTAKVKM"
     gene            17936..18814
                     /gene="yihT"
                     /locus_tag="NBI74_001304"
     CDS             17936..18814
                     /gene="yihT"
                     /locus_tag="NBI74_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfofructosephosphate aldolase"
                     /protein_id="HCD1931667.1"
                     /translation="MNKYTINDITRASGGFAMLAVDQREAMRMMFAAAGAPAPVADSV
                     LTDFKVNAAKTLSPYASAILVDQQFCYRQVVEQNAIAKSCAMIVAADEFIPGNGIPVD
                     SVVIDRKINPLQIKQDGGKALKLLVLWRSDEDAQQRLDMVKEFNELCHSHGLVSIIEP
                     VVRPPRRGDKFDREQAIIDAAKELGDSGADLYKVEMPLYGKGPQQELLSASQRLNDHI
                     NMPWVILSSGVDEKLFPRAVRVAMTAGASGFLAGRAVWASVVGLPDNELMLRDVCAPK
                     LQQLGDIVDEMMAKRR"
     gene            18830..20071
                     /gene="yihS"
                     /locus_tag="NBI74_001305"
     CDS             18830..20071
                     /gene="yihS"
                     /locus_tag="NBI74_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfoquinovose isomerase"
                     /protein_id="HCD1931668.1"
                     /translation="MKWFNTLSHNRWLEQETDRIFDFGKKSVVPTGFGWLGNKGQIKE
                     EMGTHLWITARMLHVYSVAAAMGRPGAYSLVDHGIKAMNGALRDKKYGGWYACVNDEG
                     VVDASKQGYQHFFALLGAASAVTTGHPEARKLLDYTIEIIEKYFWSEEEQMCLESWDE
                     AFSKTEEYRGGNANMHAVEAFLIVYDVTHDKKWLDRAIRVASVIIHDVARNNHYRVNE
                     HFDTQWNPLPDYNKDNPAHRFRAFGGTPGHWIEWGRLMLHIHAALEARCEQPPAWLLE
                     DAKGLFNATVRDAWAPDGADGIVYTVDWEGKPVVRERVRWPIVEAMGTAYALYTVTGD
                     RQYETWYQTWWDYCIKYLMDYENGSWWQELDADNKVTTKVWDGKQDIYHLLHCLVIPR
                     IPLAPGLAPAVAAGLLDINAK"
     gene            20138..21040
                     /locus_tag="NBI74_001306"
     CDS             20138..21040
                     /locus_tag="NBI74_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001417111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose-1-epimerase"
                     /protein_id="HCD1931669.1"
                     /translation="MQITNMHCSGQTVSLAAGDYHATIVTVGAGLAELTFQGCHLVIP
                     HKPEEMPLAHLGKVLIPWPNRIANGCYRYQGQEYQLPINEHGSKAAIHGLLAWRDWQI
                     SELSATSVTLTAFLPPSYGYPFMLASQVVYSLNARTGLSVEIASQNIGTVAAPYGVGI
                     HPYLTCNLTSVDEYLFQLPANQVYAIDEHANPTTLHHVDELDLNFTQAKKIAATKIDH
                     TFKTANDLWEMTITHPQQALSVSLCSDQPWVQVYSGEKLQRQGLAVEPMSCPPNAFNS
                     GIDLLLLEPGKTHRLFFNIHGQHN"
     gene            21101..21976
                     /locus_tag="NBI74_001307"
     CDS             21101..21976
                     /locus_tag="NBI74_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HCD1931670.1"
                     /translation="MDFFTLFSPDGVMLETNVVCIVIALMFLYTFVGICAGFGGGLTT
                     MPLVTLMLPVKMATPLSVIVGTATALYATWLSRKETDWRSAMVLIIFSFLGIPVGLYA
                     LSYLPDHFMKVGLGGFLILYSFYSMFIPRLPIYDRSWIAAPIGAVAGALGAAFSTNGP
                     PMVIYGMLRNLGPAAFRGTLNAFFTANNIAVIGGLATSGILTVSIFKLVLFCIPTMIL
                     GSLVGQYVHKRISVKVFRVMVFLLLIASGSMLIKGALGIPAAAALLPPFTLLVILQLA
                     FGKKVAAIRRVEESK"
     gene            22082..24118
                     /gene="yihQ"
                     /locus_tag="NBI74_001308"
     CDS             22082..24118
                     /gene="yihQ"
                     /locus_tag="NBI74_001308"
                     /EC_number="3.2.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfoquinovosidase"
                     /protein_id="HCD1931671.1"
                     /translation="MDTPRPQLLDFQFHQNNDSFTLRFQDRLILIHSKDNPCLWIGSG
                     IADIDMFRGNFSIKDKLQEKIALTDAIVSQSPDGWLIHFSRGSDISATLNISADDQGR
                     LLLELQNDNLNHNRIWLRLAAQPEDHIYGCGEQFSYFDLRGKPFPLWTSEQGVGRNKQ
                     TYVTWQADCKENAGGDYYWTFFPQPTFVSTQKYYCHVDNSCYMNFDFSAPEYHELALW
                     EDKATLRFECADTYISLLEKLTALLGRQPELPDWIYDGVTLGIQGGTEVCQKKLDTMR
                     NAGVKVNGIWAQDWSGIRMTSFGKRVMWNWKWNSENYPQLDSRIKQWNKEGVQFLAYI
                     NPYVASDKDLCEEAAKRGYLAKDAAGGDYLVEFGEFYGGVVDLTNPEAYAWFKEVIKK
                     NMIELGCGGWMADFGEYLPTDTYLHNGVSAEIMHNAWPALWAKCNYEALEETGKLGEI
                     LFFMRAGSAGSQKYSTMMWAGDQNVDWSLDDGLASVVPAALSLAMTGHGLHHSDIGGY
                     TTLFEMKRSKELLLRWCDFSAFTPMMRTHEGNRPGDNWQFDGDAETIAHFARMTTVFT
                     TLKPYLKEAVALNAKSGLPVMRPLFLHYEDDVQTYTLKYQYLLGRDILVAPVHEEGRS
                     DWTLYLPEDNWVHAWTGEAFRGGEVTVNAPIGKPPVFYRAGSEWAALFASLKSI"
     gene            24164..25549
                     /locus_tag="NBI74_001309"
     CDS             24164..25549
                     /locus_tag="NBI74_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCD1931672.1"
                     /translation="MSHITTEDPATLRLPFKEKLSYGIGDLASNILLDIGTLYLLKFY
                     TDVLGLPGTYGGIIFLISKFFTAFTDMGTGIMLDSRRKIGPKGKFRPFILYASFPVTL
                     LAIANFVGTPFDVTGKTVMATILFMLYGLFFSMMNCSYGAMVPAITKNPNERASLAAW
                     RQGGATLGLLLCTVGFVPVMNLIEGNQQLGYIFAATLFSLFGLLFMWICYSGVKERYV
                     ETQPTNPAQKPGLLQSFRAIAGNRPLFILCIANLCTLGAFNVKLAIQVYYTQYVLNDP
                     ILLSYMGFFSMGCIFIGVFLMPGAVRRFGKKKVYIGGLLIWVLGDLLNYFFGGGSVSF
                     VAFSCLAFFGSAFVNSLNWALVSDTVEYGEWRTGVRSEGTVYTGFTFFRKVSQALAGF
                     FPGWMLTQIGYVPNVAQADHTIEGLRQLIFIYPSALAVVTIVAMGCFYSLNEKMYVRI
                     VEEIEARKRTA"
     gene            25592..26995
                     /locus_tag="NBI74_001310"
     CDS             25592..26995
                     /locus_tag="NBI74_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCD1931673.1"
                     /translation="MSDHNPLTLKLNLREKIAYGMGDVGSNLMLSIGTLYLLKFYTDE
                     LGMPAYYGGIIFLVAKFFTAFTDMLTGFLLDSRKNIGPKGKFRPFILYAAIPAALIAT
                     LQFIATTFSLPVKTTIATALFMMFGLSYSLMNCSYGAMIPAITKNPNERAQLAAYRQG
                     GATIGLLICTVAFIPLQSLFSDSTVGYACAALMFSIGGFIFMMLCYRGVKEHYVDTTP
                     TGHKASILKSFCAIFRNPPLLVLCIANLCTLAAFNIKLAIQVYYTQYVLNDINLLSWM
                     GFFSMGCILVGVLLVPLTVKCFGKKQVYLAGMVLWAVGDILNYCWGSNSFTFVMFSCV
                     AFFGTAFVNSLNWALVPDTVDYGEWKTGIRAEGSVYTGYTFFRKISAALAGFLPGIML
                     TQIGYVPNIAQSDATLQGLRQLIFIWPCALAIIAALTMGFFYTLNEKRFALIIEEINQ
                     RKNKEIETEEKTASVTL"
     gene            27063..27755
                     /gene="ompL"
                     /locus_tag="NBI74_001311"
     CDS             27063..27755
                     /gene="ompL"
                     /locus_tag="NBI74_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpL"
                     /protein_id="HCD1931674.1"
                     /translation="MKNINAIILLSSLTSASVFAGAYVENREAYNLASDQGEVMLRVG
                     YNFDMGAGIMLTNTYTFQREDELKHGYNEIEGWYPLFKPTDKLTIQPGGLINDKSIGS
                     GGAVYLDVNYKFTPWFNLTVRNRYNHNNYSSTDLNGELDNNDTYEIGTYWNFKITEKF
                     SYTFEPHYFMRVNDFNSSNGKDHHWEITNTFRYRINEHWLPYFELRWLDRNVEPYHRE
                     QNQIRIGTKYFF"
     gene            complement(27858..29681)
                     /gene="typA"
                     /locus_tag="NBI74_001312"
     CDS             complement(27858..29681)
                     /gene="typA"
                     /locus_tag="NBI74_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-dependent GTPase TypA"
                     /protein_id="HCD1931675.1"
                     /translation="MIEKLRNIAIIAHVDHGKTTLVDKLLQQSGTFDSRAETQERVMD
                     SNDLEKERGITILAKNTAIKWNDYRINIVDTPGHADFGGEVERVMSMVDSVLLVVDAF
                     DGPMPQTRFVTKKAFAYGLKPIVVINKVDRPGARPDWVVDQVFDLFVNLDATDEQLDF
                     PIVYASALNGIAGLDHEDMAEDMTPLYQAIVDHVPAPDVDLDGPFQMQISQLDYNSYV
                     GVIGIGRIKRGKVKPNQQVTIIDSEGKTRNAKVGKVLGHLGLERIETDLAEAGDIVAI
                     TGLGELNISDTVCDTQNVEALPALSVDEPTVSMFFCVNTSPFCGKEGKFVTSRQILDR
                     LNKELVHNVALRVEETEDADAFRVSGRGELHLSVLIENMRREGFELAVSRPKVIFREI
                     DGRKQEPYENVTLDVEEQHQGSVMQALGERKGDLKNMNPDGKGRVRLDYVIPSRGLIG
                     FRSEFMTMTSGTGLLYSTFSHYDDVRPGEVGQRQNGVLISNGQGKAVAFALFGLQDRG
                     KLFLGHGAEVYEGQIIGIHSRSNDLTVNCLTGKKLTNMRASGTDEAVVLVPPIRMTLE
                     QALEFIDDDELVEVTPTSIRIRKRHLTENDRRRANRAPKDD"
     gene            30054..31463
                     /gene="glnA"
                     /locus_tag="NBI74_001313"
     CDS             30054..31463
                     /gene="glnA"
                     /locus_tag="NBI74_001313"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--ammonia ligase"
                     /protein_id="HCD1931676.1"
                     /translation="MSAEHVLTMLNEHEVKFVDLRFTDTKGKEQHVTIPAHQVNAEFF
                     EEGKMFDGSSIGGWKGINESDMVLMPDASTAVIDPFFADSTLIIRCDILEPGTLQGYD
                     RDPRSIAKRAEDYLRSTGIADTVLFGPEPEFFLFDDIRFGSSISGSHVAIDDIEGAWN
                     SSTQYEGGNKGHRPAVKGGYFPVPPVDSAQDIRSEMCLVMEQMGLVVEAHHHEVATAG
                     QNEVATRFNTMTKKADEIQIYKYVVHNVAHRFGKTATFMPKPMFGDNGSGMHCHMSLS
                     KNGVNLFAGDKYAGLSEQALYYIGGVIKHAKAINALANPTTNSYKRLVPGYEAPVMLA
                     YSARNRSASIRIPVVSSPKARRIEVRFPDPAANPYLCFAALLMAGLDGIKNKIHPGEA
                     MDKNLYDLPPEEAKEIPQVAGSLEEALNELDLDREFLKAGGVFTDEAIDAYIALRREE
                     DDRVRMTPHPVEFELYYSV"
     gene            31637..32686
                     /gene="glnL"
                     /locus_tag="NBI74_001314"
     CDS             31637..32686
                     /gene="glnL"
                     /locus_tag="NBI74_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(II)"
                     /protein_id="HCD1931677.1"
                     /translation="MATGTQPDAGQILNSLINSILLIDDNLAIHYANPAAQQLLAQSS
                     RKLFGTPLPELLSYFSLNIELMQESLEAGQGFTDNEVTLVIDGRSHILSVTAQRMPDG
                     MILLEMAPMDNQRRLSQEQLQHAQQVAARDLVRGLAHEIKNPLGGLRGAAQLLSKALP
                     DPSLLEYTKVIIEQADRLRNLVDRLLGPQLPGTRITESIHKVAERVVTLVSMELPNNV
                     RLIRDYDPSLPELAHDPDQIEQVLLNIVRNALQALGPEGGEIILRTRTAFQLTLHGER
                     YRLAARIDVEDNGPGIPPHLQDTLFYPMVSGREGGTGLGLSIARNLIDQHSGKIEFTS
                     WPGHTEFSVYLPIRK"
     gene            32698..34107
                     /gene="glnG"
                     /locus_tag="NBI74_001315"
     CDS             32698..34107
                     /gene="glnG"
                     /locus_tag="NBI74_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(I)"
                     /protein_id="HCD1931678.1"
                     /translation="MQRGIVWVVDDDSSIRWVLERALAGAGLTCTTFENGAEVLEALA
                     SKTPDVLLSDIRMPGMDGLALLKQIKQRHPMLPVIIMTAHSDLDAAVSAYQQGAFDYL
                     PKPFDIDEAVALVERAISHYQEQQQPRNVQLNGPTTDIIGEAPAMQDVFRIIGRLSRS
                     SISVLINGESGTGKELVAHALHRHSPRAKAPFIALNMAAIPKDLIESELFGHEKGAFT
                     GANTIRQGRFEQADGGTLFLDEIGDMPLDVQTRLLRVLADGQFYRVGGYAPVKVDVRI
                     IAATHQNLEQRVQEGKFREDLFHRLNVIRVHLPPLRERREDIPRLARHFLQVAARELG
                     VEAKLLHPETEAALTRLAWPGNVRQLENTCRWLTVMAAGQEVLIQDLPGELFESNVPE
                     STSHMQPDSWATLLAQWADRALRSGHQNLLSEAQPELERTLLTTALRHTQGHKQEAAR
                     LLGWGRNTLTRKLKELGME"
     gene            34219..34329
                     /locus_tag="NBI74_001316"
     CDS             34219..34329
                     /locus_tag="NBI74_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YshB family small membrane protein"
                     /protein_id="HCD1931679.1"
                     /translation="MLESIINLVSSGAVDSHTPQTAVAAVLCAAMIGLFS"
     gene            complement(34562..35935)
                     /gene="hemN"
                     /locus_tag="NBI74_001317"
     CDS             complement(34562..35935)
                     /gene="hemN"
                     /locus_tag="NBI74_001317"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HCD1931680.1"
                     /translation="MSVQQIDWDLALIQKYNYSGPRYTSYPTALEFSEDFGEQAFLQA
                     VARYPERPLSLYVHIPFCHKLCYFCGCNKIVTRQQHKADQYLDALEQEIVHRAPLFAG
                     RKVSQLHWGGGTPTYLNKAQISRLMKLLRENFQFNADAEISIEVDPREIELDVLDHLR
                     AEGFNRLSMGVQDFNKEVQRLVNREQDEEFIFALLNHAREIGFTSTNIDLIYGLPKQT
                     PESFAFTLKRVAELNPDRLSVFNYAHLPTIFAAQRKIKDADLPSPQQKLDILQETIAF
                     LTQSGYQFIGMDHFARPDDELAVAQREGVLHRNFQGYTTQGDTDLLGMGVSAISMIGD
                     CYAQNQKELKQYYQQVDEQGNALWRGIALTRDDCIRRDVIKSLICNFRLDYAPIEQQW
                     DLLFADYFAEDLKLLAPLAKDGLVDVDEKGIQVTAKGRLLIRNICMCFDTYLRQQARM
                     QQFSRVI"
     gene            complement(36124..36633)
                     /gene="yihI"
                     /locus_tag="NBI74_001318"
     CDS             complement(36124..36633)
                     /gene="yihI"
                     /locus_tag="NBI74_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Der GTPase-activating protein YihI"
                     /protein_id="HCD1931681.1"
                     /translation="MKPSSSNSRSKGHAKARRKTREELDQEARDRKRQKKRRGHAPGS
                     RAAGGNTTSGSKGQNAPKDPRIGSKTPIPLGVAEKVTKQHKPKSEKPMLSPQAELELL
                     ETDERLDALLERLEAGETLSAEEQSWVDAKLDRIDELMQKLGLSYDDDEEEEEDEKQE
                     DMMRLLRGN"
     gene            37215..37847
                     /locus_tag="NBI74_001319"
     CDS             37215..37847
                     /locus_tag="NBI74_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HCD1931682.1"
                     /translation="MTNLNYQQTHFVMSAPDIRHLPSDTGIEVAFAGRSNAGKSSALN
                     TLTNQKSLARTSKTPGRTQLINLFEVADGKRLVDLPGYGYAEVPEEMKRKWQRALGEY
                     LEKRQSLQGLVVLMDIRHPLKDLDQQMIEWAVDSNIAVLVLLTKADKLASGACKAQLN
                     MVREAVLAFNGDVQVETFSSLKKQGVDKLRQKLDTWFNEMQPVEETQDGE"
     gene            complement(38229..41015)
                     /gene="polA"
                     /locus_tag="NBI74_001320"
     CDS             complement(38229..41015)
                     /gene="polA"
                     /locus_tag="NBI74_001320"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HCD1931683.1"
                     /translation="MVQIPQNPLILVDGSSYLYRAYHAFPPLTNSAGEPTGAMYGVLN
                     MLRSLIMQYKPTHAAVVFDAKGKTFRDELFEHYKSHRPPMPDDLRAQIEPLHAMVKAM
                     GLPLLAVSGVEADDVIGTLAREAEKAGRPVLISTGDKDMAQLVTPNITLINTMTNTIL
                     GPEEVVNKYGVPPELIIDFLALMGDSSDNIPGVPGVGEKTAQALLQGLGGLDTLYAEP
                     EKIAGLSFRGAKTMAAKLEQNKEVAYLSYQLATIKTDVELELTCEQLEVQQPAAEELL
                     GLFKKYEFKRWTADVEAGKWLQAKGAKPAARPQETSVADEAPEVTATVISYDNYVTIL
                     DEETLKAWIAKLEKAPVFAFDTETDSLDNISANLVGLSFAIEPGVAAYIPVAHDYLDA
                     PDQISRERALELLKPLLEDEKALKVGQNLKYDRGILANYGIELRGIAFDTMLESYILN
                     SVAGRHDMDSLAERWLKHKTITFEEIAGKGKNQLTFNQIALEEAGRYAAEDADVTLQL
                     HLKMWPDLQKHKGPLNVFENIEMPLVPVLSRIERNGVKIDPKVLHNHSEELTLRLAEL
                     EKKAHEIAGEEFNLSSTKQLQTILFEKQGIKPLKKTPGGAPSTSEEVLEELALDYPLP
                     KVILEYRGLAKLKSTYTDKLPLMINPKTGRVHTSYHQAVTATGRLSSTDPNLQNIPVR
                     NEEGRRIRQAFIAPEDYVIVSADYSQIELRIMAHLSRDKGLLTAFAEGKDIHRATAAE
                     VFGLPLETVTSEQRRSAKAINFGLIYGMSAFGLARQLNIPRKEAQKYMDLYFERYPGV
                     LEYMERTRAQAKEQGYVETLDGRRLYLPDIKSSNGARRAAAERAAINAPMQGTAADII
                     KRAMIAVDAWLQAEQPRVRMIMQVHDELVFEVHKDDVDAVAKQIHQLMENCTRLDVPL
                     LVEVGSGENWDQAH"
     gene            41379..42311
                     /locus_tag="NBI74_001321"
     CDS             41379..42311
                     /locus_tag="NBI74_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HCD1931684.1"
                     /translation="MANFLNKFIMTRILAAITLLLSIVLTILVTIFCSVPIIIAGIVK
                     LLLPVPVIWRKVSRFCDFMMYCWCEGLAVLLHLNPHLQWEVHGLEGLSKKNWYLLICN
                     HRSWADIVVLCVLFRKHIPMNKYFLKQQLAWVPFLGLACWALDMPFMKRYSRAYLLRH
                     PERRGKDVETTRRSCEKFRLHPTTIVNFVEGSRFTQEKHLQTHSTFQNLLPPKAAGIA
                     MALNVLGKQFDKLLNVTLCYPDNNRQPFFDMLSGKLTRIVVHVDLQPISDELHGDYIN
                     DKSFKRHFQQWLNSLWQEKDRLLTSLMSSQRQDK"
     gene            complement(42352..43782)
                     /locus_tag="NBI74_001322"
     CDS             complement(42352..43782)
                     /locus_tag="NBI74_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HCD1931685.1"
                     /translation="MIRKSATGVIVALAVIWGGGTWYTGTQIQPGVEKFIKDFNDAKK
                     KGEHAYDMTLSYQNFDKGFFNSRFQMQMTFDNGAPDLNIKPGQKVVFDVDVEHGPLPI
                     TMLMHGNVIPALAAAKVNLVNNELTQPLFIAAKNKSPVEATLRFAFGGSFSTTLDVTP
                     AKYGKFSFGEGQFTFNGDGSSLSNLDIEGKVEDIVLELSPLNKVTAKSFTIDSLTRLE
                     EKKFPVGESESKFNQINIINHGEDVAQIDAFVAKTRLDRVKDKDYINVNLTYELDKLT
                     KGNQQLGSGEWSLIAESIDPSAVRQFVIQYNIAMQKQLAAHPELANDEVALQEVNAAL
                     FKEYLPLLQKSEPTIKQPVRWKNALGELNANLDISIADPAKSSSSTNKDIKSLNFDVK
                     LPLNVVTETAKQLNLSEGMDAEKAQKRADKQISGMMTLGQMFQLITIDNNTASLQLRY
                     TPGKVAFNGQEMSEEEFMSRAGRFVH"
BASE COUNT        10727 a        11165 c        10824 g        11129 t
ORIGIN      
        1 atccagaatc agaattgcgg tatttcttta attatgaacc aaacgctgcg gatgggtata
       61 aaccgtcgcc cgacccggtt tacaaaagcc caccagcgtc agattacagc gctcagccac
      121 ttctacagca agtgtggtcg cggcagacac cgcaaacaaa atctctacgc cgcacatcgc
      181 cgacttttgc accatttcat aactggcacg gctggaaacc agcaccgcac cttgctgcca
      241 gctttcccct tcttgtgacc ggcggcctaa cagtttgtcc agcgccacat ggcgccccac
      301 gtcttcatgc ccgccgacca gttcgccaga tggcaacatc catgcagcgg cgtgagtaca
      361 accagtcagt tgccccactg gctggaaatc attgagatga cgtaatgcat catccagttt
      421 gttgagatca aacgtctggg tgaacggcag cggctgcacc ggtttaccga tatcgttaag
      481 ctgttctaca ccgcatacgc cacagcccgt acgtcccgcc agcgcccggc ggcgttcctt
      541 caaccccata aagcggcggc tggaaagctc aatttgtact tccaggccat tacaggaagg
      601 aacgacatcc atgccgaaga tatcgcgcgg gctttcgata attccttcgg aaagcgaaaa
      661 accgagcgca aagtactcaa ggtctttggg cgacgccatc atcaccacgt gcgaaatgcc
      721 gttgtagacc agcgcaacgg gaacttcttc cgccacctca tctaaccgtg ggtgttgtaa
      781 atcatcacgc cgccataact caatttgacg aacacctgtg atatttgtca cattctcgat
      841 ttctttttgc tgtgtttttt tcactttcta ttaaccagat aagaacacac acaccaacat
      901 tatggtattc tgttacaaac ccttcctgga tggagggaaa ttgagccaat tctggacctt
      961 tgcggcccct tccgcaaaga aaaataactc ccactccctg cacacgcagc aagcgaatgt
     1021 aaatgggacg tgacaatgtc gaaacaagga gcaatccatg caggtcagca gaaggcagtt
     1081 ctttaagatc tgcgctggcg gtatggcagg caccacggca gccgcactgg gttttgcacc
     1141 cagcgtagca ctcgcggaaa cccggcagta taaactgttg cgcacccgcg aaacccgtaa
     1201 tacctgcacc tattgttccg taggctgtgg gctgttgatg tacagcctcg gtgacggagc
     1261 aaaaaacgcc aaagcatcta tcttccatat cgaaggtgac ccggatcacc cggtcaaccg
     1321 cggagcactt tgtccgaaag gcgctggcct ggtggatttc atccactccg aaagccgtct
     1381 gaagtttccg gaataccgtg cgccaggttc tgataaatgg cagcaaatca gttgggaaga
     1441 ggcgtttgat cgcatcgcca aactgatgaa agaagaccgc gatgcgaact acattgcgca
     1501 aaacgccgaa ggcgtgactg ttaaccgctg gctctccacc gggatgctgt gtgcttccgc
     1561 gtcgagcaac gaaaccggct atttaacgca aaaattctcc cgcgcgctgg gtatgctcgc
     1621 ggtcgacaac caggcgcgtg tctgacacgg accaacggta gcaagtcttg ctccaacatt
     1681 tggtcgcggt gcgatgacca accactgggt cgacatcaag aatgccaacc tcgtcgtggt
     1741 gatgggcggt aacgccgctg aagctcaccc ggtcgggttc cgctgggcga tggaagccaa
     1801 aattcacaac ggcgcgaagc tgattgtgat cgatcctcgc tttacgcgta cagcggcggt
     1861 ggctgactac tatgccccta tccgttccgg tactgacatt gctttcctgt caggcgtatt
     1921 gctgtacctg ctgaacaatg aaaaattcaa ccgcgaatac accgaagcct ataccaacgc
     1981 cagcctgatc gtgcgtgagg attacggctt tgaagatggc ctgttcaccg gctacgacgc
     2041 ggaaaaacgc aagtacgata agtccacctg gacttacgaa ctggacgaaa acggcttcgc
     2101 caaacgcgat actaccctgc aacatccacg ttgcgtgtgg aacttgctga aacagcacgt
     2161 ttcccgctac acgccagatg tggttgaaaa catctgcggt acgccaaaag acgcgttcct
     2221 gaaagtctgc gaatacatcg ccgaaaccag tgctcacgat aaaacagcgt cgttcctcta
     2281 cgccctcggc tggacgcaac actccatcgg tgcgcagaac atccgtacga tggcgatgat
     2341 ccagttgctg ctcggcaata tggggatggc tggcggcggc gttaacgccc tgcgcggtca
     2401 ctccaatatt caggggctga cggacctggg gctgctgtcg cagagtctgc caggttacat
     2461 gacgttgcca agcgagaagc agaccgatct gcaaacttac cttaccgcca acacgccgaa
     2521 accactgctg gaaggccagg tcaactactg gggcaactat ccgaaattct tcgtctcaat
     2581 gatgaaggca ttctttggtg ataaagcgac ggcggaaaat agctggggct ttgactggtt
     2641 gccgaagtgg gataaaggct acgacgtcct gcaatacttc gagatgatga aagagggcaa
     2701 ggtcaatggc tatatctgcc agggctttaa ccctgttgcc tcattcccga acaaaaacaa
     2761 agtgataggc tgtctgtcga agctgaaatt cctcgtgact atcgacccgc tgaacactga
     2821 aacctctaac ttctggcaga accacggtga gctgaacgaa gtggactcgt cgaagatcca
     2881 gaccgaagtg ttccgtctgc catcgacctg cttcgcggaa gagaacggtt caatcgttaa
     2941 ctctggtcgc tggttgcagt ggcactggaa aggtgcggac gccccgggga ttgcgctgac
     3001 tgatggcgag atcctctccg gtatcttcct gcgcttgcgc aagatgtatg ccgaacaggg
     3061 tggcgcgaac ccggaccagg tgctgaacat gacctggaac tacgccattc cgcatgagcc
     3121 atcttcagaa gaagtggcga tggagagcaa cggcaaagcg ctggccgata ttaccgaccc
     3181 ggcaaccggt gcggttatcg tcaagaaagg ccaacaactt agctcgttcg cccaactgcg
     3241 cgatgacgga acaacctcct gtggctgctg gattttcgcc ggtagctgga cgccggaagg
     3301 caaccagatg gcgcgtcgtg ataatgccga tccgtctggc ctcggtaaca cgctgggctg
     3361 ggcatgggca tggccgctta accgccgcat tctgtataac cgcgcctccg cagatccgca
     3421 gggtaacccg tgggatccga agcgtcagtt gctgaaatgg gacggcacta agtggaccgg
     3481 ctgggatatt ccggactaca gcgcagcgcc tccgggcagc ggcgtagggc cgtttatcat
     3541 gcagcaggaa ggcatgggac gtctgtttgc cctcgataag atggcggaag gtccgttccc
     3601 ggaacactac gagccgtttg aaacgccgct gggaactaac ccgctgcatc caaacgttat
     3661 ctcgaatccg gcggcgcgta tctttaaaga cgatgccgaa gcattgggga aagccgataa
     3721 gttcccgtat gtcggaacca cctatcgtct gaccgagcac ttccactact ggaccaaaca
     3781 cgcgctgttg aacgcgattt tgcaaccaga gcagtttgtg gaaatcgggg agtcgctggc
     3841 gaataaactt ggcattgccc agggcgatac tgtgaaagtc tcctccaacc gtggctatat
     3901 caaagccaaa gcggtagtga ccaaacgtat tcgcacgctg aaagcaaacg gcaaagatat
     3961 cgataccatc ggtattccga ttcactgggg ctatgaaggt gttgcgaaaa aaggctttat
     4021 tgccaatacg ttgacgccat tcgtcggtga tgcgaacacg cagacgccgg agtttaagtc
     4081 cttccttgtg aatgtggaaa aggtgtaacg gagacgacat atggcttatc aatcgcaaga
     4141 tatcattcgt cgttccgcga ctaacggtct gacccccgcg cctcaggcgc gggacttcca
     4201 ggaagaagtg gcgaaactca tcgacgttac cacctgtatc ggctgtaaag cctgtcaggt
     4261 ggcgtgttca gagtggaacg acattcgcga taccgtcggc aataacattg gggtgtacga
     4321 caaccccaat gatttaagtg ccaaatcgtg gacggtgatg cgattctcgg aagtggagca
     4381 gaacgacaaa ctggaatggc tgatccgcaa agacggctgt atgcactgtt ccgatccagg
     4441 ctgcctgaaa gcatgcccgg cggaaggggc aattattcag tatgccaatg gtatcgtcga
     4501 cttccagtcc gagcagtgca tcggctgcgg ttattgcatc gcagggtgcc cgttcaacgt
     4561 gccacgcctc aacccggaag acaaccgcgt ctacaaatgt acgctgtgcg ttgaccgcgt
     4621 ggtggttggg caagaaccgg cctgcgtgaa gacctgccca acgggcgcga ttcacttcgg
     4681 tacgaaagag tcgatgaaaa cgctggcgag cgagcgcgtg gcggagctga aaacccgcgg
     4741 ttacgacaat gcgggtctgt acgatccggc aggcgtcggt ggtacacacg ttatgtacgt
     4801 gctgcaccat gctgacaagc caaatctgta tcatggcttg ccggagaacc cggaaatcag
     4861 cgaaaccgtg aaattctgga aaggcatctg gaaaccactc gcagctgttg gctttgcggc
     4921 taccttcgca gccagtatct tccactacgt gggtgtcggt ccgaaccgtg cggatgagga
     4981 agagaataat ctgcacgaag agaaagacga ggagcgcaaa tgaaacgacg tgacaccatc
     5041 gtgcgctaca ccgcgccgga acgtatcaac cactggatca ccgccttctg cttcatccta
     5101 gcagcggtga gcgggctggg ctttttgttc ccgtccttca actggttgat gcaaatcatg
     5161 ggcacaccgc agctggcgcg aattctgcat ccgtttgtcg gcgtggttat gtttgcctcg
     5221 ttcatcatca tgtttttccg ttactggcat cacaacctaa tcaatcggga tgatatcttt
     5281 tgggcgaaga atattcgtaa gatcgtcgtc aacgaggaag taggtgacac cgggcgttat
     5341 aacttcggtc agaaatgcgt tttctgggcg gcgattattt tcctggtcct gttgctggtg
     5401 agcggcgtga ttatctggcg tccttatttt gcgcctgctt tctcaatccc ggtgatccga
     5461 ttcgcgttaa tgctgcattc atttgccgca gtggcgttaa ttgtggttat catggtgcat
     5521 atctacgccg ccctttgggt gaaaggcacg attaccgcca tggtggaagg atgggtgacc
     5581 agcgcatggg caaagaaaca tcacccgcgc tggtaccgtg aggtccgcaa gacaacggaa
     5641 aagaaagctg aatgagtatt cgcataatcc cgcaagatga gctgggttcg agcgagaaac
     5701 gtacggcgga tatgattccg ccgttattgt tccctcggct caagaattta tacaaccgcc
     5761 gcgccgagcg tctgcgcgag ctggcagaac ataatccact gggtgattac ctgcgctttg
     5821 ctgcgcttat cgcccacgcc caggaagtgg tgctgtacga ccatccgctg gagatggatc
     5881 tgaccacgcg cattaaagaa gccagcgcac aaggcaagcc cccgctggat attcacgttc
     5941 tgccgcgtga taagcactgg caaaagctgc tgatggcgct gattgctgag ctgaaacctg
     6001 aaatgagcgg cccggcgctg gcagtgattg agaatctgga gaaggcatcg actcaggagc
     6061 tggaagatat ggccagcgca ctgtttgcct ctgatttctc gtccgtcagc agcgataaag
     6121 cgccgtttat ctgggctgcg ctgtcgctct actgggcgca gatggctaat ctgatccccg
     6181 gcaaagcccg cgctgaatac ggcgaacaac gtcaatattg cccggtatgt ggctctatgc
     6241 cggtgtccag catggtgcaa attggcacca ctcagggtct gcgttacctg cactgcaacc
     6301 tgtgtgaaac tgaatggcac gtagtgcgcg taaaatgcag caactgtgaa cagagcggca
     6361 aactgcatta ctggtcgctg gatgacgaac aggccgcgat taaagccgaa agctgcgatg
     6421 actgcggcac ttacctgaag attctttatc aggaaaaaga accgaaagtt gaagccgtgg
     6481 cagatgacct cgcctctctg gtactggacg cgcgaatgga gcaagaaggc tacgcccgta
     6541 gttccattaa cccgttcctg tttccgggtg aaggagagta atctctgacg ttttccgggt
     6601 ggcgcaggtc gcccggatta cagttcatag ggtgtgacga cactatctct cgtattccgc
     6661 gttacctcct caagctatgc cgccagtgcc tgtttggcct caattttcac gctgagaaaa
     6721 tcgataagga tatcgatatc aaatagccaa atctttttgc cattaccatt tctcgcgcag
     6781 cttacgctgg tattcgacac catcttcctc acacccttgc cagatgccag gaacactggt
     6841 tcttgctgtt tgcgattgat ttaccaggta ttgatctacc gcttcccgta ataggtctgc
     6901 acgcggaaga ttacgctgca cctcaagatc atcaagttgc ttaatcacct cattcgataa
     6961 atcgagtaaa attctgctca tatccatacc tgccagaggg ttcataaata tctcgccaat
     7021 atcattttga atctatggag agaaaagtac ccttgtcgaa tctttaaaga aagcgcattt
     7081 acgcatcact ttttattttt gcgaggcgtt ttccagatac gatgtaacag ttgcagttat
     7141 tacgaaaacc agtaaaaaat ggaagtgaaa acagtcgtgt gaaaatttcc actctttctg
     7201 aatttactct ataaagttgc ctttctcaca tccaatagct taatgtcatc taccaggaaa
     7261 aaacgataag tcgtcaggga acggaacaga tataatgaat aaaaacgaat caatttctta
     7321 caccgaaatg caaaaatttt ttgccagaga aattaaggaa gtaggcggta ggcccatcac
     7381 ttctaagaat ttagagttaa gtctgctacc cattcacgcc agggaaaacc tttctgcgcc
     7441 agaaatgccg tggtataccg aagttaatga tatctatatg gagttcagac gctcaccatt
     7501 ctggatggtt gccggttatg tattcctggg atttatgctt tcactacttt ccatctcgat
     7561 attatctatt gttgcattct cattttatga gatgggcttt aatagtgact tgttatttct
     7621 gtatttactt gcatttgtgc ttggctgttt tatattacct ggcgctgcaa aaattgcttt
     7681 tttcacgcca cattatcttc ctataagatt taacagaaaa acacaaaaaa tttatgtttc
     7741 agatttaatt attgaaggca atgtcttctt tggaaaaata aaactcactt ttcatgaata
     7801 tgactggaaa gatgttgaag gttgggaggt gagtagacat ttcagtaaaa gtatgcctta
     7861 caatggatta catctggtag ttaatagaga aaatgatata aatgcactgc gacagacacg
     7921 cgtttataca acccgtgcac cgtggagtgc acaagaaatg gcaagacaaa agaactatat
     7981 cccccaaata tggctttatt gccataatta tatggcttat aaaactgtgt ctggtgcaaa
     8041 gaacaaacca caaccattta tttttttaag aaacaattgg ctatttaaat ggccaaaaga
     8101 aatggataaa cgttccagaa gctcaaagat actgtctgaa taagcataat aatgaaggat
     8161 ttcgactatg attaacaaac ttactcttcc ctggccggta ttatttttag aattttttgt
     8221 gcgagaagtt gcctttaagg gtaagggaat tgttcgttct acattaccag cagatgccca
     8281 tttttatacc ggtgggtcaa aagccctgcg ttatcaccta caaccaggaa accaatggcc
     8341 aaactatata tggcatgatg atataggtga tattgtaccg tttcctctac cctcgcttcc
     8401 gcttacattt accgctaacc gacccacatt taattccaga gaattagcgg ataaatggat
     8461 gaaggataat attcataccg ctgtcatcat ggcgggggaa tttctgtctg ctaatgaatg
     8521 gattcgccgc aaaaaccagc ttaaaactag tgtacaccct gcgtcagttg cttctttcgc
     8581 accctatggc tttaacatta accagtgtga tgacgatggc aatctttgga ctaatttccc
     8641 acgtccccac atcaaattaa aaatcgcacc aggaaaaaca gcttgttttc tctgccgccg
     8701 ggaagagctt ttatttggtt atgatgaagc acataatcac taccagccga atcttaatct
     8761 ggttttattt gtgaaaaatc atgggttgca taaggatgtt tacctaaaat ctcatgacga
     8821 taaaagtgaa tacgatagca gaatattatg taacgaaaaa tttaccactt atgatttccg
     8881 taaaaaccgg tccagataca gcttttgata gtttattatc ctggatgata tcaggagcga
     8941 tatctataaa gtttatgcat tccctgtgat caatttttcc tgttagtgag cacaaattca
     9001 atctgattga tgccagaata aaggcatctg gcatgttgat tttatgttcc agaatgacaa
     9061 aacattgctg ttaataagca cgactctttt tgtcattcct catgccactt accctgttct
     9121 tcaacaccaa ctacctcacc cctttgcctg tcactttttg ccttacgcac cggaaattgc
     9181 tgacgctcca gatattgctc taccgcttcg cgtaaaattg cagagcgcga aaccttacgc
     9241 cagccccgaa gcttgttcag ccgtttaatt gcctcttctg ataaatgaag aaaaaccgta
     9301 ttcatcgcca ttgccaccac cttacatata ttactcatta atgtatgtgc gaaaattacg
     9361 ccgaaaaatc atttaaaaaa agcggatttt tgcatgtcgt tttgtttttg cgagactgca
     9421 tccaaagata atgtaacagt tgcagttatt tcaattgtta ctcaaaaatg gtttattact
     9481 tttaattaag cgggggcaca tctacagaaa agaaacaaca aataataagg atgaattatg
     9541 gatgctataa atattttata ttcaagaata tttttttgat tgggtcttca tttgcttcat
     9601 caggatggtg tacccgaaaa agtcagcagc tttaccgcac cagggtttaa ggtgaaatct
     9661 accattcata acgaccggtc agtgccgcac caaccggtct ttaattgtta gtagtagtaa
     9721 atttgtggat aacagtccgg gaaccagacg attccttctg gatcatcccc ctgcattacc
     9781 cgatgacatt ggcgtcgatc atctgcgcga cggcgaagcg tagtatagag ccgcttccat
     9841 tcatgatcgc ccggtgtact gtagtttacc caacaatcca ttgaagggtg acgccattta
     9901 cctgtaagtg ttgggatccc tgaatgctta tgcggcttcg ctttaccatt acgatcccta
     9961 tctttacgcg tattaaacgt ggacattttt agtctcctgt taagagttct aaaaatgtgc
    10021 ttccttaaaa tagttcatca ttttccctta attcgcatag cgtgcttttg cacgaatttc
    10081 cgatacgcgg ggaccattat gattgcgtcg atgcccacac atccaacaag aacaaataca
    10141 gggagtacga aagcaaatac ctgatgcttt tttgcttcca tcgcctgcag tgcgataacg
    10201 acagcggatt ttttttaatc gcaccatatg atggcgacgt tcagcacgat tacgggtcat
    10261 agcaaaactc ctgagcaggc ggcactatgc cgctttcata ggagttctga atgctggtgc
    10321 tttctgtatg gcataaccat gactctaacg gttaagtgct tgcagcacat gttgcggatc
    10381 attggcaatc gctcgcagca aagcctttgc aggcccggtc ggtgaacgtc gcccctgctc
    10441 ccagttacgc aacgtgtcta cgcttacgga aataagatcg gcaaatttag attgtgacaa
    10501 ccctgaagcg cggcgtattt ctttgatttt catagcatca acttcaaaag tccgtgaagg
    10561 ctgtcgcgtt ccttcaataa tttcattcat ctgggccatg ctgtgagtca accgctcaaa
    10621 aagtgcctta tccatttacc acctcgtatt taacaaccgg agcattgcct tttccagggg
    10681 agacagatca tctttaatac ccttacgata aataagcagt aatctgatct cataatgttt
    10741 aacctgatga aagtaaataa ccctgacacc agcacgtttc cccctttcct cctgagaccc
    10801 atcgaacctt tcttaaccca ccagtttcag gtattacctc accatagtca ggattcaagg
    10861 ccaggaaaaa ctgaaaacga ctgaattcat cgtcgttcag cagtttttga acatcttcag
    10921 taaaaatttc cgtttcgatg aagagcataa ttatacgcca ctggcttaga gtaacaatcc
    10981 tttttgtacg tcattgacgt acaattgaaa ccactagctt actgatagtg ctccagcaaa
    11041 accacgtctc tgccgggttt gtctttacat ttgcgaggcg ctttccagat ttttcacgcc
    11101 cgtgaatttc cctgtatcca tccagcaaaa acgggtgtat aaaactgtac attcatacag
    11161 caaaatgagt tttactatgg cactggaaaa aggaattgca gagttagtcg aagagtttat
    11221 cgctgcgggt cgcccctcat cgcgccaaca aagtattacg cagcgcagag agggctatat
    11281 cgccagcgct gtccttgcgg gtgagacaga aacgcgggta gatattcaga ctatcgaact
    11341 tgaagggatg accttacgaa tagtgtcacc gctgaatgcc ccgacattac tcccgacgat
    11401 aatttattac tacggtggct gttttgttag cggcgggttc gcaactcacg acaaccagct
    11461 gcgccagtta gcctattacg ggcaatgtcg gattattgcc gtccagtatc ggctggcacc
    11521 ggaacatact ttccccgccg cccatgatga tgcacaacga ggtgcagaat tagtccggca
    11581 acacgccgaa cgactcggcg ttgataaaca acgaataact cttgccggtg acagtgcggg
    11641 agggcatctg gcgctggtaa ctgctttgcg attgaagcgt gctggcgagt ggcaaccagc
    11701 acaactcatc ttaatttatc ctatgcttga tgcgactgcg catttcgaaa gctacatacg
    11761 caatggtcac gattacatca tcacacgcga cactttactg agcggctttg agatgtacct
    11821 gccaggcatt gaacgcaggc atcctgaagc cagccctatt tggcgcaatg atttcaatgg
    11881 cttgccacct gtacatatca ttaccgctga atacgaccca ctttgcgatg aaggtgaagc
    11941 gttgtatcac cgcatgacag ggcaaggagt gcaatgcacc tgccaacgct accttggcgt
    12001 tatccacgga ttttttcagt taggtggtat cagtgaggct gcgcgatcgg cgctacggga
    12061 tgttgcctgg agagcgggaa ggtaactccg gacctgggcc cagaaaaggt tttcagattg
    12121 aaaacctttt cagtcaagta aaacgcccct actcttcttc gttcccgccc tcttcatacg
    12181 ggccaaatgg cttcgcgggc agaacgatat acgtgccttc aaacactgca cccggcgtct
    12241 cgtcgccaaa gatttctact tgcatctgca cccgtgcttt tcgtccgcgc gccagacggt
    12301 cgagatcgcc gcttaaggca ccaaggtcgg ctaccgcatg gggttttccg ctaatcggtt
    12361 tgctatagcg gatatgcgca tccgccagaa taatcgttcc gccgaggtgg cgttcgcgca
    12421 gcatcagcca gataagcccc caaccggtga gcgttgccag cgagaataaa ctcccggcaa
    12481 acagcgtatg gtgcggattc tgattgccgg tttccggcat ggtggtgata aatttttgcc
    12541 cggtatattg ctgaatgcgc acacccattt tttcgcttaa cgggatatgt tcataccacg
    12601 cttgctggag ctgggcgcac cagtcgccgc gatgaagaat atcatccaga gtggcgacgg
    12661 gtttaatcat cagaaaatgg cgaatcggcg tggtggttgg cgtggtgatt tcaccctgat
    12721 taacaaaccc cagcttggcg aaaaactcca ccgcgtcttc acgggcgcta caggtcacgc
    12781 gcttaacgcc ttcctgacgt gccaccgact ccagggtcat cgccatcagc gtgcctaacc
    12841 ctttgtcctg cacgtcggga tgaacggcca taaagcgaat ggacgcttca ttgtcggcat
    12901 taatatacag tcggcctacc gctaccagat taccctgctc gtcgacgacc atctggtgat
    12961 gcgccatcgc atcccacgcg tcgcgttccg aaccttttgg ttgatgcagg ggcttacgca
    13021 acatttccca gcgaaactga tagtaacgct ctaattcttc ttctgtttgt ggaacccgaa
    13081 ggtgatacat agcgatactc tctcttgttt cccgtgacaa ccctggaagc tggctcatac
    13141 ctgcaaccag aatgtcacgg ggccatcatt gaccagcgat acctgcatat ccgcagcgaa
    13201 gcgtcctgtt tgcgtgttca tatcttgctg acggcagcgt tcgacgaaat agtcatataa
    13261 cgcctctgcg cgatccggtg atgcgccttt ggagaaactt gggcgcatcc cccgttcggt
    13321 atctgcggca agggtaaact gggaaaccac cagtacactg ccgcccgcct gttgcacgtt
    13381 gagattcatc ttgccttcgg catcgctaaa gatgcggtag ccgagtacac gctcgcacag
    13441 acggtttgct ttctgttcgt cgtcatcctt ttcgacaccc aataacacca aaagtcccgc
    13501 gccaatttca cccgtcactt ctccctccac ggtgacgctg gcacgggtta cgcgttgaat
    13561 taatgcaatc atggttcgtc ttcttcttct tgttcagcag cttgttttaa tttgcggtat
    13621 tccccgagag tgacagtaat ttccgcgcca agcaagacga tacaccatgt ccagtagacc
    13681 caaacaaaga gaatggggat caccgccagc acgccgtaaa tgagctgata tgacgggaac
    13741 atggtgatat aaagcgcgaa acctttcttt cctgcttcga acaggagcgc ggcgacaaac
    13801 gcgccgacaa tcgcgtcgcg gttaggtaca cggatggtag gaacgatgct gtacagcagc
    13861 cagaaagaga tccacgacaa cagcagcgga aaaatacgca acacattatc gatgacagta
    13921 ttgagatcgc tcgcccagcg cagggaaagc agataggagc tgatcgccag actggcccct
    13981 gccagcagcg gccccagcgt taaaatcatc cagtacacgg caaacgagta aattttgggt
    14041 cgcgctcgtt tactgcgcca gatggtattc aacgcgctat cgatggagta catcaacaat
    14101 aacgccgtga cgatcagccc gcacgccccg acggcggtca ttttgttgga attggcgaca
    14161 aactgttcga tatatcgctg gataacatcg ccagtagcag gcagaaagtt ggcaaaaata
    14221 aagtgacgca actgaatgct gacgtcggaa aacatgggaa aagcggcgaa aagcgcaaaa
    14281 acaacggcaa ccagcggcac taatgagagc aacgacacat aggcaaggtt acctgccagg
    14341 gttgtcatgt tgtcctcatc aatgcgttgc cagagtagtt ttagccaggc ccatagtggg
    14401 cgggtacgat gcctggcttt gtcctgaatg gtttttagca taacaccttc gcgaaatagt
    14461 ccgggatggt ggttttatct ttcaccagaa tactagtaat gcctagccga ttagctcctt
    14521 ctatattatc ggcgttatcg tcgaaaaaga ccgtatcgtc tggtgaaaaa ccttctgcct
    14581 gcaaaacatg ctgataaatt cgtgcttcag gtttgcgcat acccaagtct tgagacagat
    14641 agatatggtc agcagcatca cggatttccg ggtattcctc cggccagaag gtagtatgca
    14701 ggcggttagt attggaaagc accaccacgc gatgcccctg ctcacgcagt ttatgcatga
    14761 tggcgatcac ttccggtcgt agcgcaacaa ataccgcctg ccaaccgtgg gagaactgct
    14821 catagcttag cggcagggcc atctcatgac acagtgcctc tgcgaacgct tcgtcgctaa
    14881 tttccccacg ctcatgctga tgaaacgcct cgcccatgtg gaaactcttc ttaagcgtag
    14941 ccagtggaac acgcgttaaa tcgctccagg cccccagcac acggttaaag tcgatatcga
    15001 caatcacatt acctaaatca aagatataga gcatttttgc ctcctttcgc gccacaagaa
    15061 attaactgta gcgggaaagg atgggtttgg ctatgtggcc aggtgtgaag ccaggtcaac
    15121 ggtcacaaaa ttctacgcgt cttcctgggg aaggatgatt tcaatttggc tgtcttgtaa
    15181 gcgtgatttt agcgcagcgg gcggcagctc gtcggtaaag agcgcagtga cctgtgccac
    15241 gttaccaatt tcaaccgccg ccgaagcatg atacttagtg tgatcggcga ccagcaggat
    15301 atttctcgcg tgcgccatca tcgttttcac cacgttagct tcgttaacat caaactccat
    15361 caacgcgcca tcgctctcga ttgcgcctac gcttgttacc agataatcgg cgcgaaaatc
    15421 ggccacaaag gacgccgctg aagggccaat gatcccactg ttatgggaac gcagcgttcc
    15481 gccaggcacc atcacttcaa agtgcgggtt gtggtaaaga atatgcgcca cacgcaggct
    15541 attggtgatt atccgcaaat ggttatggtt aagtaacgcc cgggcaacat gctcaacggt
    15601 cgtaccaatg gtgataaata ttgttgaacc atcaggaata taatctgcca ccgcttcggc
    15661 aatcgctttt ttttcttcgg tttgcgaaac ctcacgctgc tcgaacgccg tattaacgac
    15721 gctggaagcc cgacccgcgc caccgtgatg gcgcgtaatc agtccttgct cgcttaattt
    15781 acgaatatcc cggcgcaccg tctgcgtgga gacatccagc agatttgcca gctcatcaat
    15841 attcatatac ccacgctcgg cgatcagcat gaggagttgg tcgtgccgcg ggttaccggt
    15901 tagttcggta aggctcatga aaattcctcg aaaaaccatc accctggcat tttatacaaa
    15961 aagtgacaaa aaagatcggg tttgatcaca gtcagggatc ccggctcgtc caccgggacg
    16021 cgtgcatttc aacgccgcta ctccgctggc gaagcggact gactccgcta aatccccacc
    16081 tgttgccagc gccaccgcca atgcgccgtg aaaaacatca cccgcacctg tggtatctac
    16141 cacatcaact ttgaaggccg gttgatgctg tcgcccgcca ttctccagcc agtcacagcc
    16201 tgcactgccc tgggtcacat agacatgacc atttgtgagc atttgtgcct gttttagggc
    16261 actcaccatc tctttcacgc ccgttaagcg cgccagaccc ggttctgaaa aagctgcatg
    16321 atcgcttaac gccaccagtt cgctgatatc ctgcggcgta atatcaccgt ccagcacggt
    16381 catcacgccc gcctgccgcg ccagagtgaa ggcttttttc gcgccgtcgt gccagcgtac
    16441 atctgccagc accacgtccc actgagagaa atcaatttcc tccaaccact ctgcatcagg
    16501 cagcaggtct gggctgggat agttaatgat gattcgctca cctttggcat ccaccatgat
    16561 ggcggattgc gaagatttcg cctggttata ccgtttggtg taacgggtgt taacgcccca
    16621 ggattccagc tctgccagca ggctgttgcc cgtgtcgtcg tcaccgacgc gaccaataaa
    16681 atcgacctgc gcacctagtc tggccgccgc aactgctgct gtagccgctg gcccgccgcc
    16741 aacttccgta taatttttcg ccacgtattt accgctctcc gtcggcaatc cttccacgta
    16801 atagatgcga tccatcacgg taattcctac acaagcaaca cgaatcatgg tcattcctta
    16861 agcattttgg gatgtgttca ttttaatttc atcaattgtt taaaaagtga cggctgtcaa
    16921 ttttttgacg ataaattaca aatacgatca aaaacagaca agcaaggcat gtcataaatg
    16981 acaaaaagtg acatcactgt attcaggaga gagttatggc agcaatcgcg tttatcggtt
    17041 tagggcaaat gggttcgcca atggcgagca atttattgca gcaagggcat caactttgcg
    17101 tcttcgatgt gaataccgaa gccgtacagc atctggtcga caaaggggcg actcccgctg
    17161 ctaacccagc gcaggccgct aaaggtgccg aatttatcat taccatgttg ccgaatggcg
    17221 atctggtgcg tcacgtgttg ttcggtgaaa acggcgtttg tgagagctta tcttccgatg
    17281 cgctggtcat tgatatgtcg accatccacc cactgcaaac cgataaattg attgctgata
    17341 tgcaggccag aggcttcagc atgatggatg ttccggttgg ccgtacttcc gcaaatgcca
    17401 ttaccggtac tctgctactg ctggctggcg gcactgctga acaagttgaa cgcgccacgc
    17461 cgatcctgat ggcgatgggc agtgagttga tcaacgcggg cggtccgggc atggggatcc
    17521 gcgttaagct tatcaacaac tacatgagca tcgcgctcaa tgcgttgtcg gcagaagccg
    17581 ccgttttgtg cgaagccctg aatcttccct tcgatgttgc cgtcaaggta atgagcggta
    17641 ctgctgccgg taaaggccac ttcaccactt cctggccgaa caaagtcctc agtggagatc
    17701 tttctcccgc cttcatgatc gatcttgccc ataaggatct tggcatcgcc cttgatgtcg
    17761 ccaaccagtt gcatgtgccg atgccactgg gggccgcctc acgggaggtt tatagccagg
    17821 cgcgcgcagc gggtcgcggt cgccaggact ggtccgccat tctggagcag gtccgtgtca
    17881 gtgccgggat gactgccaaa gtaaaaatgt aacgactgga taaaggaata aatgaatgaa
    17941 taagtacacc atcaacgaca ttacgcgtgc atcgggcggt tttgccatgc tggcggtcga
    18001 tcagcgcgaa gccatgcgca tgatgtttgc cgcggctggc gcacccgctc cagtagccga
    18061 tagcgtttta actgatttca aagttaacgc cgcgaagact ctttcacctt atgcatcggc
    18121 gattctggta gatcaacaat tctgctaccg ccaggtggtt gagcaaaacg cgattgccaa
    18181 aagttgcgcc atgattgtcg ccgccgatga gttcattcct ggcaacggta ttccggtaga
    18241 tagcgtggtc attgaccgca aaatcaatcc gctacaaatc aaacaagacg gtggcaaagc
    18301 cttaaaactg ctggtgctgt ggcgtagtga tgaagatgcg cagcaacgcc tggatatggt
    18361 gaaagagttt aacgaactgt gccactcaca cggtctggta agcatcattg agccagtcgt
    18421 ccgcccaccg cgtcgtggcg ataaattcga tcgcgaacaa gcaatcatcg atgcagccaa
    18481 agagctgggc gacagtggcg ctgacctcta caaagttgaa atgccgcttt acggtaaagg
    18541 tccgcaacaa gagcttctca gtgcttcaca acgtctgaat gaccatatca atatgccgtg
    18601 ggtgatcctc tcttccggtg tcgacgaaaa actgtttccg cgtgccgtac gcgtggcaat
    18661 gacagcgggt gcatcgggct tcctggctgg tcgtgccgtc tgggcatcgg tcgtcggttt
    18721 accagacaac gagctgatgc tgcgtgacgt ttgcgcaccg aaattacaac aacttggcga
    18781 tatcgtcgac gaaatgatgg ctaaacgccg ttaagatggg gatacaaaaa tgaaatggtt
    18841 taacacttta agtcacaacc gttggctgga acaggaaacc gatcgcatct ttgattttgg
    18901 taaaaaatcc gtagtaccga ccggatttgg ctggttaggc aataaagggc aaatcaaaga
    18961 agagatgggc acccatctgt ggatcacagc tcgtatgttg cacgtttatt ccgttgctgc
    19021 ggcgatgggc cgacctggcg cttactcgtt ggttgatcac ggtatcaaag ccatgaatgg
    19081 cgcactgcgc gataaaaaat atggcggttg gtatgcctgc gtgaatgacg agggcgtggt
    19141 ggatgcctcc aaacagggct atcaacattt ctttgctctg ctgggtgctg ccagcgccgt
    19201 cactaccggt catccggaag cgcgcaagct gctcgattac accattgaaa ttatcgagaa
    19261 atacttctgg agcgaagaag agcagatgtg cctggagtcc tgggatgaag ccttcagcaa
    19321 aaccgaagag tatcgcggcg gcaatgccaa tatgcacgcg gtggaagcct tcctgattgt
    19381 ctatgacgtc acccatgaca aaaaatggct ggatcgcgcg attcgcgtgg cttccgtgat
    19441 tatccacgac gtcgccagaa ataatcatta tcgcgttaac gaacacttcg atacccagtg
    19501 gaatccgctg ccagattaca acaaagataa cccggcgcac cgcttccgcg cgttcggagg
    19561 tactccgggc cactggatcg aatggggccg tttaatgctg cacattcacg cggccctgga
    19621 agcccgttgc gaacaaccgc cagcatggct gctagaagat gccaaaggtc tgtttaacgc
    19681 caccgtgcgc gatgcctggg cacccgatgg cgcggacggg attgtttata ccgttgactg
    19741 ggaaggaaaa ccggtggtcc gcgaacgtgt gcgttggcct atcgtcgaag cgatgggtac
    19801 ggcctacgcg ctctacaccg tcactggcga tcgccagtac gaaacttggt atcagacatg
    19861 gtgggactac tgcatcaaat acctgatgga ctatgaaaat ggttcctggt ggcaggagct
    19921 ggatgcggac aataaggtca ccaccaaagt ctgggacggc aaacaggata tttatcatct
    19981 gctgcattgt ctggtgatcc cacgtatccc gttagcgcca ggcctggctc cggcggttgc
    20041 ggcgggtctg ctggatatta acgcgaaata actgactaaa aaaacgacat atcaggctgc
    20101 acgccgggta tgtcgttatt caataaaggt acctcgtatg caaatcacca acatgcattg
    20161 cagtggacag accgtttcac ttgccgcggg ggattatcac gcgaccattg tgaccgtcgg
    20221 tgcaggtctg gctgaattaa cctttcaggg ctgtcattta gttattccgc ataaacccga
    20281 agagatgccg ctggcacatc tgggcaaagt ccttattccg tggccaaacc ggatcgccaa
    20341 cggctgttat cgctatcagg ggcaggaata tcagttaccc attaatgaac atggttcaaa
    20401 agccgctatt catgggctgc tcgcctggcg tgactggcaa atcagcgaat taagcgccac
    20461 cagcgtcaca ttaaccgcct ttctgccacc cagttacggc tacccgttta tgctggcttc
    20521 gcaggtggtt tattcgctca acgcccgcac gggtctttcc gttgagatcg caagccagaa
    20581 tatcggcact gtcgcagcgc cttacggtgt gggtattcac ccttatttaa cctgcaactt
    20641 aacatctgtt gatgaatatc tgttccagct tccggcaaac caagtttatg ccatcgatga
    20701 gcacgctaat ccgacaacgc ttcaccacgt tgatgagctg gatttaaatt tcacgcaggc
    20761 gaaaaaaata gccgcgacaa aaatcgacca cacctttaaa accgctaatg atctgtggga
    20821 aatgactatt acccatccac agcaggcgct ttcggtgagc ctctgttccg atcaaccctg
    20881 ggtgcaggtt tatagcggtg aaaaattaca acgtcaggga ctggcagttg aaccaatgag
    20941 ttgcccgcct aacgccttta attcaggtat cgacttatta ttactggaac cagggaaaac
    21001 gcaccgcctg ttttttaata ttcacgggca gcacaattaa ttttattaag aaaagaacga
    21061 gatcattgtt tagagttgta tttacacaac aggaaatatt atggactttt ttacgttatt
    21121 ttcaccggat ggggtcatgc tggaaacaaa cgtggtatgt atcgttatcg cgttaatgtt
    21181 tctttatacc tttgtgggga tatgcgcggg atttggcggt ggtttaacca ccatgccgct
    21241 cgtcacgtta atgctccccg taaaaatggc aacgccgctc tcggtgattg tcggcaccgc
    21301 gaccgccttg tacgccacct ggctgtcacg taaagaaacg gattggcgat cggcaatggt
    21361 actgattatt ttctcgtttc tgggaatacc cgtcggttta tatgcgctgt cgtatttacc
    21421 cgaccatttt atgaaagtgg gcttaggcgg attccttatc ctgtactcct tttacagtat
    21481 gtttattcca cgcctgccga tttatgaccg tagctggatc gccgcaccta tcggcgccgt
    21541 ggcaggagcg ttaggggcag cattttcaac caacggaccg ccgatggtta tctacggtat
    21601 gctgcgtaac ttaggcccgg cggcatttcg cggcactctg aacgctttct tcaccgcaaa
    21661 caacatcgcg gtgatcggcg ggctggcgac cagcggaatt ttgactgtct ctatctttaa
    21721 actggtgctg ttttgtattc ccaccatgat cctcggttca ctggttggac aatatgtgca
    21781 taaacgcatt tcggtcaaag tgttccgtgt gatggtcttt ttattgctga ttgcctccgg
    21841 ttctatgttg ataaaaggcg cgctgggaat tcccgccgct gccgcacttc ttcctccgtt
    21901 taccttgtta gttatactgc aactggcatt tggtaaaaaa gtggcggcaa tccgccgggt
    21961 tgaagaaagc aaataatcat tatgcgggtt ttaatttata cccgcacaca ttctttaatt
    22021 aatgaagcct gtaataacag gatggcatga aatattagtt ttatatctat ggagattatt
    22081 tatggatacg ccacgcccac agctattaga ttttcaattt catcagaata acgacagttt
    22141 tactctacgt tttcaagacc gccttatttt aatccatagc aaagataatc cttgtttatg
    22201 gattggttca ggtatagcgg atatcgatat gttccgcggt aatttcagca ttaaagataa
    22261 attgcaggag aaaattgcgc ttaccgacgc catcgtcagc cagtcaccgg atggctggtt
    22321 aatccatttc agccgtggtt ctgacattag cgccacgctg aatatctctg ccgacgatca
    22381 gggccgttta ttgctggaac tacaaaacga caaccttaac cacaaccgta tctggctgcg
    22441 ccttgccgca cagccggaag atcatatcta cggctgcggc gaacaattct cctactttga
    22501 tctgcgcggc aaaccgttcc cgctatggac cagtgaacaa ggcgttggtc gcaacaaaca
    22561 aacctatgtc acctggcagg ccgactgcaa agagaatgcg ggaggggatt actactggac
    22621 cttctttcca caacccacgt ttgttagcac gcagaagtat tactgccatg ttgataacag
    22681 ttgctatatg aacttcgact ttagcgcgcc ggaataccat gaactggcgc tgtgggaaga
    22741 caaagcaaca ctgcgttttg aatgtgctga tacatacatc tccctgctgg aaaaattaac
    22801 cgcgctgctg ggacgccagc cagaactgcc cgactggatt tatgacggag taacgctcgg
    22861 cattcagggc ggcactgagg tgtgccagaa gaaactggac accatgcgta acgcgggcgt
    22921 gaaggtcaac ggcatctggg cgcaggattg gtccggtatt cgtatgacct cgtttggcaa
    22981 acgcgtaatg tggaactgga agtggaacag cgaaaactac ccgcaactgg attcacgtat
    23041 caagcagtgg aacaaagagg gcgtacagtt ccttgcctat atcaacccgt atgttgccag
    23101 cgataaagac ctctgcgaag aagcggcaaa gcgcggctat ctggcaaaag atgccgccgg
    23161 cggcgattat ctggtggagt ttggcgagtt ctacggcggc gttgtcgatc tcactaatcc
    23221 ggaagcctac gcctggttca aggaagtgat caaaaagaac atgattgaac tcggctgcgg
    23281 cggctggatg gctgacttcg gcgagtatct gcccaccgac acttacctgc ataacggcgt
    23341 cagcgccgaa attatgcata acgcctggcc cgcgctgtgg gcgaaatgta actacgaagc
    23401 ccttgaagaa acgggcaagc tcggcgagat cctcttcttt atgcgcgccg gttctgccgg
    23461 tagccagaaa tactccacca tgatgtgggc gggcgaccag aacgttgact ggagtctgga
    23521 cgatggactg gcgtctgtcg tcccggcggc gctgtcgctg gcaatgaccg gacatggcct
    23581 gcatcacagt gatattggcg gttacaccac cctgtttgag atgaagcgca gcaaagagct
    23641 gctgctgcgc tggtgcgatt tcagcgcctt cacgccgatg atgcgcaccc atgaaggtaa
    23701 ccgtcctggc gacaactggc agtttgacgg cgacgcggaa actatcgccc attttgcccg
    23761 tatgaccacc gtcttcacta ccctgaaacc ttacctgaaa gaggccgtcg cgctgaatgc
    23821 gaagtccggc ctgccggtta tgcgcccact gttcctgcat tacgaagacg acgtgcagac
    23881 ctacaccctg aaatatcagt acctgttagg ccgcgacatt ctggtcgctc cggtgcatga
    23941 agaaggccgt agcgactgga cgctctatct gccggaggat aactgggtcc acgcctggac
    24001 gggtgaagcg ttccggggcg gggaagtgac cgttaatgca cccatcggta aaccgccggt
    24061 cttttatcgc gccggtagcg aatgggcggc actgttcgcg tcattaaaaa gcatctaatc
    24121 acatccgccc gtggggaaac tcacgggcaa agggagaaca acgatgagtc acatcacaac
    24181 ggaagatccg gctactttac gcctgccctt taaagagaaa ctctcttacg gtatcggcga
    24241 tctggcctct aacatcctac tggatatcgg cacgctttat cttttgaagt tttataccga
    24301 tgttctgggg ctgcctggca cctatggcgg cattatcttt ttgatttcaa aattctttac
    24361 cgcctttacc gatatgggta ccggcattat gttggattcc cgacgcaaga tcggtccaaa
    24421 aggtaagttc cgtcctttta ttctgtatgc gtcatttccg gtcaccctgc tggcgatcgc
    24481 caactttgtc ggcacaccgt ttgatgtcac cggtaaaacg gtgatggcca ccatcctgtt
    24541 tatgctttac ggactgtttt tcagcatgat gaactgctcg tatggcgcga tggtccccgc
    24601 tattactaaa aaccccaacg aacgcgcctc actggcggca tggcgtcagg gtggcgctac
    24661 attgggcctg ctgctttgta cggtgggatt cgtgccggtt atgaatctta tcgagggtaa
    24721 tcagcaactt ggctatatct tcgccgccac gctgttttca ctgttcggcc tgctgtttat
    24781 gtggatctgc tattcgggcg tgaaagagcg ttatgtcgaa acccaaccta ccaatccggc
    24841 gcaaaagcct ggcttgttgc aatctttccg cgcaattgcg gggaaccgcc cactgttcat
    24901 tttgtgcatt gccaacctct gtaccttagg ggcgtttaac gtcaagctcg ccattcaggt
    24961 ctattacacc cagtacgtgc ttaacgatcc catcctgttg tcatatatgg gattttttag
    25021 catgggctgt attttcatcg gcgtgttcct gatgcctggt gcagtcagac gttttggtaa
    25081 gaagaaggtc tatatcggcg gtctgctgat ctgggtgctg ggcgatctgc tcaactattt
    25141 cttcggcggc ggctcggtca gctttgtggc tttctcctgc ctggcgttct ttggttcggc
    25201 gtttgttaac agcctgaact gggcgctggt ttctgacacc gtcgagtatg gcgagtggcg
    25261 caccggtgtg cgttcggaag gtacggtcta caccggtttc accttctttc gcaaagtatc
    25321 tcaggcgctg gcaggtttct tcccaggctg gatgctgacg caaattggct atgtgccaaa
    25381 cgtcgcccag gctgaccaca ctatcgaagg gttgcgccaa ctgatcttca tctacccaag
    25441 cgcactggca gtagtcacca ttgtggcaat gggttgcttc tacagtctga acgagaagat
    25501 gtacgtccgc attgttgaag agatagaagc ccgtaaacgc acggcgtaat tataataaac
    25561 aacgccctgc ggggcgttat aaggagtgat tatgtctgac cataatccac tgacattaaa
    25621 actgaatctg cgggaaaaaa tcgcctatgg tatgggcgac gtcggttcga atttaatgct
    25681 tagcatcggc actctgtatc tcctcaaatt ttataccgat gaattaggga tgcctgctta
    25741 ctatggcggg attatctttc tggtggcgaa gttttttacc gcatttaccg atatgctcac
    25801 cggattttta ctcgactcgc ggaaaaatat tggacctaaa ggaaaattca ggccgtttat
    25861 tttatacgca gcgattccgg cggcgttaat tgcaacgctc cagtttatcg ccaccacctt
    25921 tagtttgccg gttaaaacga cgattgccac cgcactgttt atgatgtttg gactttcata
    25981 cagtctgatg aactgctcgt atggcgcgat gatcccggcc attaccaaaa acccgaatga
    26041 gcgcgcgcaa ctcgctgctt accgccaggg tggtgcaacc atagggctat tgatctgtac
    26101 cgtggcgttt attcctttgc agtcactatt ttctgactcc accgtcggct acgcctgcgc
    26161 ggcactgatg ttctccattg gcggctttat ttttatgatg ctgtgctaca gaggtgtcaa
    26221 agagcattat gtggacacga cgccaaccgg acataaggcc agtattctca aatcattttg
    26281 cgcgatattt cgtaatccac ctttgctggt attgtgcatt gctaacctgt gtaccctggc
    26341 ggcatttaat atcaaactgg cgattcaggt ctattacacc cagtatgtgc tgaatgatat
    26401 taacttattg tcgtggatgg gattctttag tatgggatgc attctcgtcg gcgtattact
    26461 ggtgccacta actgtaaaat gttttggtaa aaaacaggtt tatctggctg gcatggtgct
    26521 gtgggcggtg ggggatatcc tgaattattg ctggggcagt aactcattca ctttcgtcat
    26581 gttctcttgt gtcgcctttt ttggcacggc ctttgtaaac agcctgaact gggcactggt
    26641 accagatacc gtagattatg gtgaatggaa aacaggtata cgtgccgaag gttctgttta
    26701 taccggttat accttctttc gtaaaatttc agccgcactt gctggcttct tgccaggcat
    26761 tatgctgacg caaatcggtt atgttcccaa catcgcgcaa agcgatgcaa cattacaggg
    26821 tttgcgtcag ctcattttta tctggccttg tgcgctggca attattgctg cattgacgat
    26881 ggggttcttt tacacgctca atgagaaacg gtttgcactg attattgagg aaattaacca
    26941 acgcaaaaat aaagaaatcg agacagaaga aaaaacggct tccgtaacgt tataaatgtc
    27001 aatagccgct atttccatcc tgatgggtgg cggcctccct acgtttaaaa aatggactta
    27061 ttatgaaaaa tattaatgca ataattttat tatcttctct gacctctgca tcagtattcg
    27121 ctggcgcata cgtcgaaaac agggaagcat ataatctcgc ctccgatcag ggtgaggtta
    27181 tgctacgtgt gggttataac ttcgatatgg gcgcgggtat tatgctaacc aatacctaca
    27241 cctttcagcg agaagatgaa ctcaaacatg gctataatga aattgaaggt tggtatcctt
    27301 tatttaaacc taccgataaa ttaactatcc agcccggtgg cttaattaat gacaagagta
    27361 tcggttcggg tggtgctgtt tatctggatg ttaactacaa atttactccg tggtttaacc
    27421 tgacggtacg taatcgctat aaccataata attacagctc tacagatttg aacggcgaat
    27481 tggataataa cgacacctat gaaattggca cctactggaa ctttaaaata acagaaaaat
    27541 tttcctatac atttgaacca cattacttca tgcgagtgaa tgactttaat agtagcaacg
    27601 ggaaagatca tcattgggaa atcactaaca ccttccgtta ccgtattaat gaacactggc
    27661 ttccttattt cgaattgcgc tggttagacc gcaacgtcga gccgtaccac cgcgagcaaa
    27721 accagattcg tatcggaacg aagtatttct tctgatgtaa aaattcctga tgcgatgctg
    27781 gcgcatctta tcaggtctac aaaagccaaa aaaaaacctg ccatcgctgg caggtttttt
    27841 atgactgaaa aacgaaatta atcgtctttc ggtgcgcggt tagcgcgacg acgatcgttt
    27901 tccgtcaggt gacgtttacg aatacggata gaggtcggag tcacttctac cagttcgtca
    27961 tcatcgatga actccagagc ttgttccaga gtcatgcgga taggcggaac cagaacaacg
    28021 gcttcgtcag taccggaagc acgcatgttg gtcagtttct taccggtcag gcagtttaca
    28081 gtcaggtcgt tagagcggct gtgaataccg ataatctgac cttcgtaaac ttcagcaccg
    28141 tgaccgagga acagcttacc gcgatcctgc agaccgaaca gcgcgaacgc gaccgcttta
    28201 ccctgaccgt tagagatcag tacgccgttc tgacgctggc ccacttcacc cggacgtacg
    28261 tcgtcgtagt ggctgaaggt ggagtacagc agaccagtac cggaagtcat ggtcatgaac
    28321 tcagaacgga agccaatcag accgcggctt gggatcacgt agtcgagacg tacgcggcct
    28381 ttaccgtctg gattcatgtt tttcaggtcg cctttacgtt cgcccagcgc ctgcattaca
    28441 gaaccctgat gctgttcttc aacgtccaga gtcacgtttt catacggctc ttgtttacga
    28501 ccgtcgattt cacggaagat aactttcgga cgggataccg ccagttcgaa accttcacga
    28561 cgcatgtttt cgatcagaac agacaggtgc agttcgccac gaccagaaac gcggaacgca
    28621 tcggcgtctt cggtttcttc tacgcgcagc gcaacgttgt gtaccagttc tttgttcaga
    28681 cgatccagga tctgacgaga cgttacgaac ttaccttctt taccgcagaa cggcgaggtg
    28741 ttaacgcaga agaacataga aacggtcggc tcatcaacgg agagtgccgg cagcgcttca
    28801 acgttttgcg tgtcgcaaac ggtgtcagaa atgttcagtt cgccaagacc cgtgatcgcc
    28861 acgatatcgc cagcttccgc cagatcggtt tcgatacgtt ccagaccgag gtggcccagc
    28921 actttaccga ctttcgcgtt gcgagttttg ccttcgctat cgatgatagt aacctgttgg
    28981 ttcggcttca ctttaccgcg cttgatgcgg ccaatgccga taacgccaac atagctgttg
    29041 taatcgagct gagaaatctg catctggaac ggaccgtcaa ggtcaacgtc cggcgcagga
    29101 acgtggtcaa caatcgcctg gtacagcggg gtcatgtctt ccgccatatc ttcgtggtcc
    29161 agacccgcga taccgttcag cgcagaagcg taaacgatcg ggaagtccag ctgctcgtcg
    29221 gtcgcgtcga ggttaacgaa cagatcgaat acctgatcca caacccaatc aggacgcgcg
    29281 ccagggcggt caactttgtt gataacaaca atcggcttca ggccgtaagc aaacgctttt
    29341 ttggttacga agcgcgtttg cggcatcggg ccgtcaaatg cgtcaaccac cagcagcact
    29401 gagtctacca tggacattac acgttcaact tcaccaccga agtcggcgtg ccccggggta
    29461 tcaacgatgt tgatacggta atcattccat ttgatagcgg tgtttttcgc gaggatggta
    29521 atcccacgct ctttctccaa atcgttggag tccatcacgc gctcttgggt ttcggcacga
    29581 gagtcgaacg taccggattg ttggagcagc ttgtctacca gggtggtttt accatggtct
    29641 acgtgcgcga tgatggcgat attacgcaat ttttcgatca caactttgcc tcaggcatta
    29701 gaaatagcgc gttattgtac acggattaat cgcactacaa aacaggatca caaacatcct
    29761 ccgcaaacaa gtattgcaga gtccctttgt gatcgctttc acggagcata aaaagggtta
    29821 tccaaaggtc attgcaccaa tatggtgctt aatgttccta ttgaagcact atattggtgc
    29881 aacattcaca tcatggtgca gcccttttgc acgatgttaa gcatgataac gccttttagg
    29941 ggcaatttaa aagttggcac agttttcgct ttatcttttt tacggcgaca cggcccattt
    30001 aattgcagat ttcgttacca cgacgacaat gaccaatcca ggagagttaa agtatgtccg
    30061 ctgaacacgt actgacgatg ctgaacgagc acgaagtgaa gtttgttgat ttgcgcttca
    30121 ccgataccaa aggtaaagaa cagcacgtca ctatccctgc tcatcaggtg aatgccgaat
    30181 tcttcgaaga aggcaaaatg tttgacggct cctcgattgg cggctggaaa ggcattaacg
    30241 aatccgacat ggtgctgatg ccagacgcat ccaccgcagt gattgacccg ttcttcgccg
    30301 attctaccct gattatccgt tgcgacatcc ttgaacctgg caccctgcaa ggctatgacc
    30361 gtgacccgcg ctctattgcg aagcgcgctg aagattacct gcgttctact ggcattgccg
    30421 acaccgtact gttcgggcca gaacctgaat tcttcctgtt cgatgacatc cgtttcggat
    30481 cgtctatctc cggttcccac gttgctatcg atgatatcga aggtgcatgg aactcctcca
    30541 cccaatatga aggtggtaac aaaggtcacc gtccggcagt gaaaggcggt tacttcccgg
    30601 ttccgccggt agactcagct caggatatcc gttctgaaat gtgcctggtg atggaacaga
    30661 tgggtctggt ggttgaagct catcaccacg aagtcgctac cgcaggtcag aacgaagtgg
    30721 ctacccgctt caataccatg accaaaaaag ctgacgaaat tcagatctac aaatatgtcg
    30781 tgcacaacgt ggcgcaccgt ttcggtaaga ccgcgacctt tatgccaaaa ccgatgttcg
    30841 gtgataacgg ttccggtatg cactgccaca tgtcgctgtc taaaaacggc gtcaacctgt
    30901 ttgctggcga caaatacgca ggtctgtctg agcaggcact gtactacatt ggcggcgtaa
    30961 tcaaacacgc taaagcgatt aacgccctgg caaacccgac caccaactct tacaagcgtc
    31021 tggtcccggg ctacgaagca ccggtaatgc tggcttactc tgcgcgtaac cgttctgcgt
    31081 ctatccgtat tccggtggtt tcttctccga aagcacgtcg tatcgaagta cgtttcccgg
    31141 acccggcggc taacccgtac ctgtgctttg ctgccctgct gatggccggt cttgatggca
    31201 tcaagaacaa gatccatccg ggcgaagcca tggacaaaaa cctgtatgac ctgccgccag
    31261 aagaagcgaa agagatccca caggttgcag gctctctgga agaagcactg aacgaactgg
    31321 atctggatcg cgagttcctg aaagccggtg gcgtgttcac ggatgaagcg atcgatgcgt
    31381 acatcgctct gcgtcgcgaa gaagatgacc gcgtgcgtat gactccgcat ccggtagagt
    31441 ttgagctgta ctacagcgtc taatagttga agttgtacta cccggcgcaa caacgccggg
    31501 atttagttgc cgtggaaact ttcagcccat ctctgcatgg gcttttttct ccgtcaattc
    31561 tctgatgctt cgcgcttttt atccgtaaaa agctataatg cactaaaatg gtgcaacctg
    31621 ttcaggagac tgctttatgg caacaggcac gcagcccgat gctgggcaga tcctcaactc
    31681 gctgattaac agtattttgt taatcgatga caacctggcg atccattacg ccaaccccgc
    31741 cgcgcaacaa ctgctcgccc aaagctcccg caaattgttt ggtacaccgt taccggaact
    31801 gttgagctac ttctcattaa atatcgagct gatgcaagaa agtctggagg cggggcaagg
    31861 ttttaccgat aacgaagtga cgctggtcat cgacgggcgc tcgcatatcc tttctgtgac
    31921 ggctcagcgt atgccggacg gcatgatcct gctggagatg gcaccgatgg ataaccagcg
    31981 ccgcttaagt caggaacagc tacagcacgc ccagcaggtt gctgcccgtg atttagtgcg
    32041 cggcctggca catgagatta aaaatccgct tggcggttta cgtggcgcgg cgcagctgct
    32101 cagcaaagca ttacctgacc cgtcactact cgaatatacc aaagtgatta tcgaacaggc
    32161 ggaccggctg cgaaatctgg tcgaccgtct gttggggccg cagctgcccg gtacgcgcat
    32221 taccgaaagt attcacaaag tggctgaacg cgtggtgacg ctggtgtcga tggaactgcc
    32281 gaacaacgtg cggttgattc gggattacga ccccagcctg ccggaactgg cgcacgaccc
    32341 ggatcaaatt gaacaggtct tgctgaatat tgtgcgcaat gcgctacagg cgctggggcc
    32401 ggagggtgga gaaatcattc tgcgtacccg cacagcgttt caactgacct tacacggcga
    32461 gcgctatcgg ctggcggcgc ggattgatgt ggaagataac gggccgggca ttccgcctca
    32521 tttgcaggat acgctgtttt acccgatggt cagcggccgc gaaggtggca ccgggcttgg
    32581 cttatccatc gcccgtaatt tgattgatca gcattcaggc aaaattgaat ttaccagttg
    32641 gccaggtcat accgagttct cggtttacct gcctatcagg aaataaaggt gacgtttatg
    32701 caacgaggga tagtctgggt agtcgatgac gatagttcca tccgttgggt gcttgaacgt
    32761 gcgctcgctg gagcgggttt aacctgtacg acatttgaga acggcgcgga agtactggag
    32821 gcgctggcga gcaaaacgcc ggatgtgctg ctttcagata tccgtatgcc gggaatggac
    32881 gggctggcgc tgctcaagca gattaaacag cgccatccaa tgcttccggt catcattatg
    32941 accgcacatt ccgatctgga tgctgccgtc agcgcctatc aacaaggggc atttgattat
    33001 ctgcccaaac cgtttgatat cgacgaagcc gtggcgctgg ttgagcgcgc catcagtcat
    33061 taccaggaac agcagcagcc gcgtaatgtt cagcttaacg gcccaacgac cgatatcatc
    33121 ggtgaagcgc cagccatgca ggacgtgttc cggattatcg gtcggctttc gcgttcttct
    33181 attagcgtgc tgattaacgg cgagtccggc accggtaaag aactggtcgc tcatgccctt
    33241 catcgccaca gtccgcgagc caaagcgcca tttatcgcgc tcaatatggc ggctatcccg
    33301 aaggatttga tcgaatccga actgtttggt cacgagaaag gcgcgtttac cggcgcgaat
    33361 accattcgtc aggggcgttt tgaacaggct gatggcggta cattatttct cgatgaaatt
    33421 ggcgatatgc cgctggatgt gcagacgcgt ttgctgcgcg tactggcaga tggtcagttt
    33481 tatcgcgttg gcggctatgc gccggtgaaa gtagatgtgc ggattatcgc tgccactcac
    33541 cagaatcttg aacagcgggt gcaggaaggc aagttccgtg aggatctgtt ccaccgcctg
    33601 aacgtcatcc gcgttcatct gccgccgctg cgcgaacgtc gggaagatat tccccgtctg
    33661 gcacgccatt ttttacaggt tgccgcgcga gagctgggcg tagaagcgaa gttgctgcat
    33721 ccggaaaccg aagccgcgct gacgcgtctg gcgtggccag gcaacgtgcg ccagctggaa
    33781 aacacctgtc gctggctaac ggtgatggcc gccgggcagg aagtgttgat tcaggatttg
    33841 cctggcgaac tgtttgaatc aaacgtaccg gaaagcactt cacacatgca gccggacagt
    33901 tgggcaacgc tgttagcaca gtgggcagac agagcgctgc gttccggtca tcaaaacctg
    33961 ctttccgaag cgcagcctga gctggagcgg acgttactga ccaccgcgtt gcgacatacg
    34021 caggggcata aacaggaagc ggcgcggcta cttggctggg gccgcaacac cctgacgcgt
    34081 aagttaaaag agctggggat ggagtgattc acggcttgtg tgtaaagatt gattattgag
    34141 cgcaaattgc tggtattttg cgctttactg ttccgataag ttcagtatga tcttgcccgg
    34201 aaaacgggga gagtcattat gctggaatca ataattaatc tggtatcgag tggcgcagtt
    34261 gacagccaca caccgcaaac tgctgttgct gccgtgctgt gtgccgcgat gatagggctg
    34321 tttagctgaa gctcgttgtt tacgccggat gcggagaaag tcgtaggcct gataagacgc
    34381 aaccagcgtc gcatcaggcg ttgattgccg gatgcggcgt aaacgcctta tccggcctac
    34441 aaaagcaata atttcaacag attttgcagg cctgataagc gtagcgcatc aggcaatctt
    34501 gttctcagca cattgatggt attttcgttt tctactttgt aaacgaagcg ccattcacaa
    34561 tttaaatcac ccgagagaac tgctgcatcc gcgcttgctg gcgcagatag gtatcaaagc
    34621 acatgcagat gttgcggatc agcaagcgac ctttcgccgt cacctgtatt cccttctcat
    34681 ccacatccac cagcccatct tttgctaacg gggcgagcag cttgagatct tccgcaaagt
    34741 aatcagcgaa gagcaaatcc cactgttgct caataggggc gtaatccaga cggaagttgc
    34801 agatgagcga cttaatcaca tcgcggcgaa tacagtcatc acgcgttagc gcaataccac
    34861 gccacagcgc attgccttgt tcatccactt gctgatagta ctgcttcaac tctttctggt
    34921 tctgcgcgta gcagtcgcca atcatgctga tggcggaaac gcccatcccc agcagatcgg
    34981 tatcgccctg agtggtgtag ccctggaagt tacgatgcag cacgccttca cgctgggcca
    35041 ccgccagctc gtcatccgga cgggcaaagt gatccatacc gataaactga tagcccgatt
    35101 gtgtcaggaa ggcgatggtt tcctgcagaa tatcgagttt ttgttgcgga ctcggcaggt
    35161 cagcatcttt gattttgcgc tgggcggcaa aaatagttgg cagatgcgcg tagttaaaga
    35221 cgctcagacg gtcagggttc agttccgcca cacgcttcag ggtaaaggcg aaactctccg
    35281 gcgtctgttt cggcaggccg taaatcaggt cgatgttggt ggaggtaaag ccaatctcac
    35341 gcgcatggtt aagcagtgca aagatgaact cttcatcctg ctcgcggtta accagacgtt
    35401 gcacttcttt gttgaagtcc tgcacgccca tgctcagccg attaaagcct tcggcgcgta
    35461 aatgatcgag tacatccagt tcgatttccc gcggatcgac ttcgatcgaa atctccgcat
    35521 cggcattgaa ctggaagttt tcgcgcagca gcttcatcag acggctgatt tgcgctttgt
    35581 tcagatatgt cggcgtacca ccgccccagt gcagctggct caccttgcgc ccggcaaaaa
    35641 gcggtgcacg atggacgatt tcttgctcca gcgcgtccag atactgatcg gccttgtgct
    35701 gctggcgagt aacaatctta ttgcaaccgc agaagtaaca aagcttatgg cagaacggaa
    35761 tatgtacgta gagagataat ggacgctcag gatagcgcgc cacggcttgt aaaaacgcct
    35821 gttcgccgaa gtcttctgaa aactccagcg cggttgggta cgaggtgtat cgtggcccgg
    35881 aatagttata tttctggatc agggccagat cccagtcgat ttgctgtaca gacatgctca
    35941 ctccttccga tggcgtctct ggcggctacg gcgtaccggc ggatgcccgt ttcgggtcat
    36001 cagccggttg cgcagccact tttgtcgccg cgacaaccgc tgtagtttaa cgaataacca
    36061 caccagataa catataaccg gaagggttat cagcagaacg ggaaggccca cggcggcaat
    36121 ccgttagttg ccccgcagca gacgcatcat gtcttcttgc ttctcgtctt cttcctcttc
    36181 ttcgtcatcg tcataagaga ggccgagttt ctgcatcaac tcatcaatac gatccagttt
    36241 ggcatccacc caggattgct cttcggcgct cagggtttcg cctgcttcca gacgttccag
    36301 cagcgcatcc agacgctcat ccgtttccag taactccaac tccgcctgcg gtgaaagcat
    36361 aggtttctca ctcttcggtt tgtgctgttt ggtgactttt tcagccacgc ccaatggaat
    36421 aggggtttta ctgccaatac gtggatcttt tggtgcgttc tggcctttgc tgcctgacgt
    36481 ggtgttacca cccgctgcgc ggctgcccgg cgcatgacca cgacgttttt tctgacgctt
    36541 acggtcacga gcttcctgat ccagctcctc gcgtgttttt cgacgcgctt ttgcatgacc
    36601 tttgctgcgt gagtttgaag atgatggttt cataatggtt cgtcttttag ccgttttatt
    36661 cagtatagat ttgcggcgga atctaacaga aagcaagcaa agaaaaaagg cgacagatta
    36721 ctctgtcgcc ttttttcctg actcataacc cttaacgggt cttacaatcc ttgcaggccg
    36781 ccaacacacc ctgtttttcc attagccgga acaatccgtg ttgattccat ttccatttaa
    36841 ttacgtctcc ggacgtttat cttcctgaca atcctctgtc ttcgcctcct ggcgctcctg
    36901 accctcatcc tgagtcattg ttcctgttag cgtcctcgct ctatgggtgc tactttacgc
    36961 ctttgcttta aataaacaat caaccgccat cagtatttgc acattttcag agttttatat
    37021 aaaagcaatt cactgattta tatagcttat ttgacatgcg tcgcagaatt ttctctgcta
    37081 tttcgcataa agactatcgg caatgtctca caaagcgtat ggcttttact tacaacgctc
    37141 acgccgaaaa atccgccttt tattatggtt caggtataat cgccgcaaag ctttgactga
    37201 tggagacgac cgctttgact aatttgaatt atcaacagac gcattttgtg atgagtgcgc
    37261 ctgatattcg ccacctacct tccgataccg gaattgaagt ggcttttgca ggccgttcca
    37321 acgcaggtaa atccagcgcg ctgaacacgc tgactaacca gaaaagcctg gcgcggacct
    37381 caaaaacccc agggcgcacc cagcttatca acctgtttga agtggctgac ggcaagcgtc
    37441 tggttgacct gcccggctat ggctacgcgg aagtcccgga agagatgaag cgcaaatggc
    37501 aacgcgcgct cggcgagtac ctcgaaaaac gtcagagcct gcaaggtctg gtggtgctaa
    37561 tggatattcg ccatccgctg aaagatttgg atcaacagat gattgagtgg gcggtagaca
    37621 gcaatatcgc cgtgctggtg ctgctgacca aagcggacaa actggcaagc ggcgcatgta
    37681 aagcgcaatt gaatatggtg cgtgaagcgg tgctggcgtt taacggtgat gtgcaggttg
    37741 aaacgttctc ttcactgaag aaacaaggcg tggacaagct gcggcagaaa ctggatacct
    37801 ggtttaacga gatgcagcct gtagaagaaa cgcaggacgg cgaataattt tcttgcctta
    37861 atgcttgtgc cggatgtggc gtatccggcc cgtaaattca atagattaca cttctaccgt
    37921 tggcctgata agcagggcgc atcaggcatt acggatcttt tctttcgccc aataaaaaac
    37981 gccccagtca ttactgactg gggcggctaa aatattcagc caaatccgat tacgtgaagt
    38041 aaaaggtctg aaagatagaa catcttacct ctgtacccta cgcgactaac tttactcttt
    38101 tttgttcagt tcagaaagca ttttttgtaa ttttttttca gttgttgcat aggaatttct
    38161 aatagccatc acaaaacgtg acagcttatg ttgcttactt acgaaaaaag ggcatgttca
    38221 ggcgaatctt agtgcgcctg atcccagttt tcgccactcc ccacttccac cagcaacggc
    38281 acatccagac gggtacagtt ttccatcagt tgatgaatct gcttcgcgac ggcatcgaca
    38341 tcatctttat gaacttcaaa taccagttca tcgtgtacct gcatgatcat acgcacacgc
    38401 ggttgctcag cctgtagcca cgcatcaacg gcaatcatcg cccgtttgat aatgtcggcg
    38461 gcggttccct gcattggcgc gttaatggct gcacgttcag ccgctgcacg acgagcccca
    38521 ttgctggatt tgatatccgg cagatacaga cggcgtccgt ccagcgtttc aacgtagccc
    38581 tgctctttcg cctgagcacg ggtgcgttcc atatactcca gcacgccagg gtagcgttcg
    38641 aagtaaaggt ccatgtactt ctgcgcttct ttacgtggaa tgttcaattg ccgcgccaga
    38701 ccgaaagcac tcatgccata aatcagacca aagttgatcg ctttcgcgct acggcgttgc
    38761 tcgctggtta cggtttccag tggcaaacca aacacttctg ccgccgtagc ccggtggata
    38821 tcttttcctt ccgcgaatgc ggtcagcaag cctttgtcac gcgagagatg cgccataatg
    38881 cgcagttcaa tctgcgagta gtccgccgag acaatcacat aatcctctgg cgcaataaac
    38941 gcctggcgga tacgacgacc ttcttcgtta cgcaccggaa tgttttgcag gttaggatcg
    39001 gttgacgata aacgtcccgt tgcagttact gcctggtgat aagaggtatg cacacgcccg
    39061 gttttcgggt tgatcatcag cggcagcttg tcggtgtaag tcgatttcag cttcgccaga
    39121 ccacgatact ccagaatcac ttttggcaac ggatagtcca gcgccagttc ttccagtacc
    39181 tcttccgacg ttgacggcgc gccacctggc gttttcttca gcggtttaat gccctgtttt
    39241 tcaaacagaa tggtttgtaa ctgcttggtg gaagaaaggt taaactcctc acctgcaatt
    39301 tcatgcgctt tcttttccag ctcagccaga cgaagagtga gctcttcaga atgattgtgc
    39361 agcactttcg gatcgatctt cacaccgtta cgttcaatgc gtgaaagcac cggcactagc
    39421 ggcatttcga tattctcgaa gacgttcaac ggccctttgt gtttttgcag atccggccac
    39481 attttcagat gcagctgcaa ggtgacatct gcatcttcgg cggcgtaacg tccggcctct
    39541 tccagggcaa tctggttaaa ggtcagttga tttttacctt taccagcaat ctcttcaaaa
    39601 gtgatggttt tatgcttcaa ccaacgttcc gcgaggctgt ccatatcgtg gcgaccggca
    39661 acgctgttga gaatgtagga ctccagcatg gtatcaaatg caatcccccg cagttcaatg
    39721 ccgtagttcg ccagaatacc gcgatcgtat ttcaggtttt gcccgacctt cagcgccttt
    39781 tcatcttcca gcagcggttt tagcaactcg agtgctcgct cgcgagagat ttgatcgggc
    39841 gcatcaagat aatcatgagc aaccggaata tatgccgcta cgcctggctc gatagcaaaa
    39901 gaaagcccga ccaggttggc agagatgtta tcgaggctgt cggtttcggt atcaaatgca
    39961 aataccggcg ctttttccag cttcgcaatc cacgctttca gtgtttcttc atcaaggatg
    40021 gtgacgtagt tgtcataaga aatcaccgtt gccgtcactt ctggcgcttc gtctgcaaca
    40081 ctggtttcct gcggcctggc tgcgggtttc gcccctttag cctgtaacca tttgcccgct
    40141 tcgacatcag cagtccagcg tttgaactca tactttttga acagccccaa caactcttcc
    40201 gccgccggtt gctgcacttc cagttgttca caggtcagct ccagttcaac gtcggtttta
    40261 atcgtcgcca gctggtatga gagataagca acttctttgt tttgctcgag cttcgctgcc
    40321 attgttttcg cgccacggaa gcttaaccca gcaatttttt ctggctcggc atacagcgta
    40381 tccagtccgc caagaccttg cagcaatgcc tgcgcggttt tttcaccgac gcccggtacg
    40441 ccaggaatgt tatcagagga gtcacccatc agcgccagga aatcgatgat cagttctggc
    40501 ggcacgccgt acttattcac cacctcttcc ggtccgagga tggtattcgt catggtattg
    40561 ataagcgtaa tatttggcgt caccagctgc gccatatctt tatcgccagt gctgatcagc
    40621 accggacgcc cggctttttc ggcttcgcgc gccagagtac cgataacgtc gtccgcttct
    40681 acgccagaaa ccgccagcag cggcagtccc atcgctttaa ccatcgcgtg caagggttcg
    40741 atttgtgcac gcagatcgtc cggcattggt gggcgatgtg atttgtaatg ttcaaacagt
    40801 tcatcacgaa aggtttttcc cttggcgtca aagaccaccg ctgcatgcgt cggtttatat
    40861 tgcatgatca gactgcgcag catgttgagg acaccataca tcgcaccggt cggctcgcct
    40921 gcgctgttag tcagcggggg aaacgcgtga tatgcgcgat aaagataaga tgaaccatct
    40981 acaaggataa gtggattttg ggggatctga accataatgt ccgtgcctgt ttatcagatt
    41041 atgggtaaag gatgccacag acaggataaa aacatgagtt tttcaccgct tttcgtgaaa
    41101 atcttttgta gatcttctgg atcgctcgca aatcgtcatg tggataactt tgtgcataaa
    41161 ttatttacat cgcttttaat ttattatcat gctttttaca gcatgatgtt tgtctttgtt
    41221 tttcatgcag ttaaaaataa agccatccct aataattgag ataatgggat tttttctaat
    41281 gtggatagat atgaattatt tttctcctta aggatcatcc gttatttggg tcgttttctg
    41341 ctaaaatctg cgccttgcgc ttctttagcg cactgtctat ggctaacttt ttgaataaat
    41401 tcattatgac gagaatactc gctgcgataa cccttttgct gagtatcgta ttgaccattt
    41461 tggtcactat tttttgctcc gtcccgatca tcattgccgg gattgtaaaa ctattgcttc
    41521 ctgtgccagt catttggcga aaggtttcgc gtttttgtga ttttatgatg tattgctggt
    41581 gcgaaggtct ggcggtatta ctgcatctga acccacacct gcaatgggaa gttcacgggc
    41641 tggaagggtt aagtaagaag aactggtatc tgcttatttg taaccaccgt agttgggcag
    41701 atattgtcgt actgtgcgtg ctgtttcgta agcacattcc gatgaataaa tatttcctca
    41761 agcagcagct ggcctgggtg ccttttcttg gcctggcgtg ctgggcgctg gatatgccgt
    41821 ttatgaagcg ttattcccgc gcttatttat tacgtcatcc ggagcgacgt ggtaaagatg
    41881 tcgaaaccac tcggcgttct tgtgaaaagt ttcgcctgca tcccaccacc attgtcaatt
    41941 tcgttgaagg ctcccgtttc acgcaagaaa aacatctgca aacccactcc acttttcaaa
    42001 acttgttgcc accaaaggcg gcaggcattg cgatggcgct aaatgtactg ggtaaacaat
    42061 tcgataaact gcttaatgtt acgctgtgtt atccggacaa taaccgtcag ccgttcttcg
    42121 atatgttaag cggtaaactg acgcgaattg tcgtacatgt ggatttgcag cctatttccg
    42181 atgagttaca tggcgactac atcaatgaca aaagctttaa gcgccatttt cagcaatggc
    42241 tgaactcact gtggcaggaa aaagaccggc ttctgacgtc gttgatgtca tcacagcggc
    42301 aggacaagta aattttgctc gcctctgaat gagcagaggc gagtgagtat tttaatgaac
    42361 aaaacgtccg gcacgagaca taaattcttc ttcgctcatc tcctgtccgt taaaagcaac
    42421 tttacccggt gtataacgca gttgcagcga ggcggtattg ttgtcaatcg tgattaactg
    42481 aaacatctga cctaatgtca tcatcccgct gatttgttta tcagcccgct tttgcgcttt
    42541 ttcagcatcc attccttcag ataaattaag ctgttttgcg gtttctgtga cgacattaag
    42601 cggtaacttc acatcaaaat tgagcgattt aatatctttg tttgtggatg atgaggattt
    42661 ggctgggtcg gcaatactga tatccagatt ggcatttagt tcgccgagtg cattcttcca
    42721 tcttaccggt tgtttaatgg tcggctcact tttttgtaat aacggtaaat actctttgaa
    42781 caatgcagca ttcacttctt gcagagcaac ttcatcgttt gctaactcag ggtgtgcagc
    42841 aagctgcttc tgcatcgcaa tgttatactg gatgacaaat tggcgcaccg ctgagggatc
    42901 aatagattca gcaatcaatg accactcacc actaccgagt tgctgattcc cttttgttaa
    42961 cttatcaagt tcgtaggtca gattgacatt gatataatct ttgtctttaa cgcgatccag
    43021 cctggttttt gcaacgaaag catcgatttg ggcaacgtct tccccgtgat tgataatgtt
    43081 aatctggtta aatttcgact cgctttcgcc aaccggaaat ttcttttctt ctaatcgcgt
    43141 tagagaatca atggtaaaac ttttcgccgt tactttgttc aatggcgata attccaaaac
    43201 aatatcttcg actttgcctt caatatccag gttagataat gaactaccat caccattaaa
    43261 agtaaactgg ccctcaccaa aagaaaactt tccatatttt gcaggggtaa catctaatgt
    43321 cgtggagaat gagccaccaa acgcgaatcg caatgtcgct tccacgggcg atttattttt
    43381 cgcggcgata aatagcggtt gtgtcagttc attattcact aagttcactt ttgccgctgc
    43441 cagtgctggg atgacattac catgcattaa cattgtgatg ggcaacggac cgtgctcaac
    43501 atccacatca aatacaactt tctggcctgg cttgatattg agatcgggtg caccgttatc
    43561 gaatgtcatt tgcatttgaa aacgggaatt aaaaaagcct ttgtcaaaat tttgataact
    43621 taacgtcata tcgtaggcat gttcaccttt ctttttagca tcgttaaaat ctttaataaa
    43681 tttttcgaca ccaggctgaa tttgcgtacc tgtgtaccat gtgccaccac cccagattac
    43741 ggctaacgca acaataacac ctgtagctga cttacgtatc atataaatat tccctgataa
    43801 aacagcaaaa ctttgaatat ccacttatgc tgaaataatg tccag
//
LOCUS       DAHHTG010000024        42289 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.024, whole genome shotgun sequence.
ACCESSION   DAHHTG010000024 DAHHTG010000000
VERSION     DAHHTG010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 42289)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42289)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42289
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.024"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            complement(46..1905)
                     /gene="gss"
                     /locus_tag="NBI74_001323"
     CDS             complement(46..1905)
                     /gene="gss"
                     /locus_tag="NBI74_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutathionylspermidine
                     amidase/synthase"
                     /protein_id="HCD1931686.1"
                     /translation="MSKGTTSQDAPFGTLLGYAPGGVAIYSSDYSSLDPQEYEDDAVF
                     RSYIDDEYMGHKWQCVEFARRFLFLNYGVVFTDVGMAWEIFSLRFLREVVNDNILPLQ
                     AFPNGSPRAPVAGALLIWDKGGEFKDTGHVAIITQLHGNKVRIAEQNVIHSPLPQGQQ
                     WTRELEMVVENGCYTLKDTFDDTTILGWMIQTEDTEYSLPQPEIAGELLKISGARLEN
                     KGQFDGKWLDEKDPLQNAYVQANGQVINQDPYHYYTITESAEQELIKATNELHLMYLH
                     ATDKVLKDDNLLALFDIPKILWPRLRLSWQRRRHHMITGRMDFCMDERGLKVYEYNAD
                     SASCHTEAGLILERWAEQGYKGNGFNPAEGLINELAGAWKHSRARPFVHIMQDKDIEE
                     NYHAQFMEQALHLAGFETRILRGLDELGWDAAGQLIDGEGRLVNCVWKTWAWETAFDQ
                     IREVSDREFAAVPIRTGHPQNEVRLIDVLLRPEVLVFEPLWTVIPGNKAILPILWSLF
                     PHHRYLLDTDFTVNDELVKTGYAVKPIAGRCGSNIDLVSHHEEVLDKTSGKFAEQKNI
                     YQQLWCLPKVDGKYIQVCTFTVGGNYGGTCLRGDESLVIKKESDIEPLIVVKE"
     gene            2110..2976
                     /gene="yghU"
                     /locus_tag="NBI74_001324"
     CDS             2110..2976
                     /gene="yghU"
                     /locus_tag="NBI74_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent disulfide-bond
                     oxidoreductase"
                     /protein_id="HCD1931687.1"
                     /translation="MTDNTYQPAKVWTWDKSAGGAFANINRPVSGPTHEKTLPVGKHP
                     LQLYSLGTPNGQKVTIMLEELLALGVTGAEYDAWLIRIGDGDQFSSGFVEVNPNSKIP
                     ALRDHTHNPPIRVFESGSILLYLAEKFGYFLPQDLAKRTETMNWLFWLQGAAPFLGGG
                     FGHFYHYAPVKIEYAINRFTMEAKRLLDVLDKQLAQHKFVAGDEYTIADMAIWPWFGN
                     VVLGGVYDAAEFLDAGSYKHVQRWAKEVGERPAVKRGRIVNRTNGPLNEQLHERHDAS
                     DFETNTEDKRQG"
     gene            complement(3099..3347)
                     /gene="hybG"
                     /locus_tag="NBI74_001325"
     CDS             complement(3099..3347)
                     /gene="hybG"
                     /locus_tag="NBI74_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation factor HybG"
                     /protein_id="HCD1931688.1"
                     /translation="MCIGVPGQVLAVGEDIHQLAQVEVCGIKRDVNIALICEGNPADL
                     LGQWVLVHVGFAMSIIDEDEAKATLDALRQMDYDITSA"
     gene            complement(3360..3701)
                     /gene="hypA"
                     /locus_tag="NBI74_001326"
     CDS             complement(3360..3701)
                     /gene="hypA"
                     /locus_tag="NBI74_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HCD1931689.1"
                     /translation="MHELSLCQSAVEIIQRQAEQHDVKRVTAVWLEIGALSCVEESAV
                     RFSFEIVCHGTVAQGCDLHIVYKPAQAWCWDCSQVVEIHQHDAQCPLCHGERLRVDTG
                     DSLIVKSIEVE"
     gene            complement(3694..4182)
                     /gene="hybE"
                     /locus_tag="NBI74_001327"
     CDS             complement(3694..4182)
                     /gene="hybE"
                     /locus_tag="NBI74_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-2 assembly chaperone"
                     /protein_id="HCD1931690.1"
                     /translation="MTEEIAGFQTSPKAQVQAAFEEIARRSMHDLSFLHPSMPVYVSD
                     FTLFEGQWTGCVITPWMLSAVIFPGPDQLWPLRKVSEKIGLQLPYGTMTFTVGELDGV
                     SQYLSCSLMSPLSHSMSIEEGQRLTDDCARMILSLPVTNPDVPHAGRRALLFGRRSGE
                     NA"
     gene            complement(4175..4669)
                     /locus_tag="NBI74_001328"
     CDS             complement(4175..4669)
                     /locus_tag="NBI74_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HyaD/HybD family hydrogenase maturation
                     endopeptidase"
                     /protein_id="HCD1931691.1"
                     /translation="MRILVLGVGNILLTDEAIGVRIVEALEQRYILPDYVEILDGGTA
                     GMELLGDMANRDHLIIADAIVSKKNTPGTMMILRDEEVPALFTNKISPHQLGLADVLS
                     ALRFTGEFPKKLTLVGVIPESLEPHIGLTPTVEAMIEPALEQILAALRESGVEAIPRE
                     AIHD"
     gene            complement(4669..6372)
                     /gene="hybC"
                     /locus_tag="NBI74_001329"
     CDS             complement(4669..6372)
                     /gene="hybC"
                     /locus_tag="NBI74_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 large subunit"
                     /protein_id="HCD1931692.1"
                     /translation="MSQRITIDPVTRIEGHLRIDCEIENGVVSKAWASGTMWRGMEEI
                     VKNRDPRDAWMIVQRICGVCTTTHALSSVRAAESALNIDVPVNAQYIRNIILAAHTTH
                     DHIVHFYQLSALDWVDITSALQADPTKASEMLKGVSTWHLNSPEEFTKVQNKIKDLVA
                     SGQLGIFANGYWGHPAMKLPPEVNLIAVAHYLQALECQRDANRVVALLGGKTPHIQNL
                     AVGGVANPINLDGLGVLNLERLMYIKSFIDKLSDFVEQVYKVDTAVIAAFYPEWLTRG
                     KGAVNYLSVPEFPTDSKNGSFLFPGGYIENADLSSYRPITSHSDEYLIKGIQESAKHS
                     WYKDEAPQAPWEGTTIPAYDGWSDDGKYSWVKSPTFYGKTVEVGPLANMLVKLAAGRE
                     STQNKLNEIVAIYQKLTGNTLEVAQLHSTLGRIIGRTVHCCELQDILQNQYSALITNI
                     GKGDHTTFVKPNIPATGEFKGVGFLEAPRGMLSHWMVIKDGIISNYQAVVPSTWNSGP
                     RNFNDDVGPYEQSLVGTPVADPNKPLEVVRTIHSFDPCMACAVHVVDADGNEVVSVKV
                     L"
     gene            complement(6369..7547)
                     /gene="hybB"
                     /locus_tag="NBI74_001330"
     CDS             complement(6369..7547)
                     /gene="hybB"
                     /locus_tag="NBI74_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase cytochrome b subunit"
                     /protein_id="HCD1931693.1"
                     /translation="MSHDPQPLGGKIISKPVMIFGPLIVICMLLIVKRLVFGLGSVSD
                     LNGGFPWGVWIAFDLLIGTGFACGGWALAWAVYVFNRGQYHPLVRPALLASLFGYSLG
                     GLSITIDVGRYWNLPYFYIPGHFNVNSVLFETAVCMTIYIGVMALEFAPALFERLGWK
                     VSLKRLNKVMFFIIALGALLPTMHQSSMGSLMISAGYKVHPLWQSYEMLPLFSLLTAF
                     IMGFSIVIFEGSLVQAGLRGNGPDEKSLFVKLTNTISVLLAIFIVLRFGELIYRDKLS
                     LAFAGDFYSVMFWIEVLLMLFPLVVLRVAKLRNDSRMLFLSALSALLGCATWRLTYSL
                     VAFNPGGGYAYFPTWEELLISIGFVAIEICAYIVLIRLLPILPPLKQNDHNRHEASKA
                     "
     gene            complement(7537..8523)
                     /gene="hybA"
                     /locus_tag="NBI74_001331"
     CDS             complement(7537..8523)
                     /gene="hybA"
                     /locus_tag="NBI74_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 operon protein HybA"
                     /protein_id="HCD1931694.1"
                     /translation="MNRRNFIKAASCGALLTGALPSVSHAAAENRPPIPGSLGMLYDS
                     TLCVGCQACVTKCQDINFPERNPQGEQTWSNNDKLSPYTNNIIQVWTSGTGVNKDQEE
                     NGYAYIKKQCMHCVDPNCVSVCPVSALKKDPKTGIVHYDKDVCTGCRYCMVACPYNVP
                     KYDYNNPFGALHKCELCNQKGVERLDKGGLPGCVEVCPAGAVIFGTREELMAEAKKRL
                     ALKPGSEYHYPRQTLKSGDTYLHTVPKYYPHLYGEKEGGGTQVLVLTGVPYENLDLPK
                     LDDLSTGARSENIQHTLYKGMMLPLAVLAGLTVLVRRNTKNDHHDGGDDHES"
     gene            complement(8526..9644)
                     /gene="hybO"
                     /locus_tag="NBI74_001332"
     CDS             complement(8526..9644)
                     /gene="hybO"
                     /locus_tag="NBI74_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 small subunit"
                     /protein_id="HCD1931695.1"
                     /translation="MTGDNTLIHSHGINRRDFMKLCAALAATMGLSSKAAAEMAESVT
                     NPQRPPVIWIGAQECTGCTESLLRATHPTVENLVLETISLEYHEVLSAAFGHQVEENK
                     HNALEKYKGQYVLVVDGSIPLKDNGIYCMVAGEPIVDHIRKAAEGAAAIIAIGSCSAW
                     GGVAAAGVNPTGAVSLQEVLPGKTVINIPGCPPNPHNFLATVAHIITYGKPPKLDDKN
                     RPTFAYGRLIHEHCERRPHFDAGRFAKEFGDEGHREGWCLYHLGCKGPETYGNCSTLQ
                     FCDVGGVWPVAIGHPCYGCNEEGIGFHKGIHQLANVENQTPRSQKPDVNAKEGGNVSA
                     GAIGLLGGVVGLVAGVSVMAVRELGRQQKKDNADSRGE"
     gene            complement(9833..10120)
                     /locus_tag="NBI74_001333"
     CDS             complement(9833..10120)
                     /locus_tag="NBI74_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2623 domain-containing protein"
                     /protein_id="HCD1931696.1"
                     /translation="MNNHFGKGLMAGLKATHADSAVNVTKFCADYKRGFVLGYSHRMY
                     EKTGDRQLSAWEAGILTRRYGLDKEMVMDFFRENNSCSTLRFFMAGYRLEN"
     gene            complement(10239..11124)
                     /locus_tag="NBI74_001334"
                     /pseudo
     CDS             complement(10239..11124)
                     /locus_tag="NBI74_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311911.3"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
     gene            11281..12321
                     /gene="gpr"
                     /locus_tag="NBI74_001335"
     CDS             11281..12321
                     /gene="gpr"
                     /locus_tag="NBI74_001335"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-glyceraldehyde 3-phosphate reductase"
                     /protein_id="HCD1931697.1"
                     /translation="MVWLANPERYGQMQYRYCGKSGLRLPALSLGLWHNFGHVNALES
                     QRAILRKAFDLGITHFDLANNYGPPPGSAEENFGRLLREDFAAYRDELIISTKAGYDM
                     WPGPYGSGGSRKYLLASLDQSLKRMGLEYVDIFYSHRVDENTPMEETASALAHAVQSG
                     KALYVGISSYSPERTQKMVELLREWKIPLLIHQPSYNLLNRWVDKSGLLDTLQNNGVG
                     CIAFTPLAQGLLTGKYLNGIPQDSRMHREGNKVRGLTPKMLTEANLNSLRLLNEMAQQ
                     RGQSMAQMALSWLLKDDRVTSVLIGASRAEQLEENVQALNNLTFSTKELAQIDQHIAD
                     GELNLWQASSDK"
     gene            complement(12361..12855)
                     /locus_tag="NBI74_001336"
     CDS             complement(12361..12855)
                     /locus_tag="NBI74_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00645 family protein"
                     /protein_id="HCD1931698.1"
                     /translation="MERFLENAMYASRWLLAPVYFGLSLALVALALKFFQEIIHVLPN
                     IFSMAESDLILVLLSLVDMTLVGGLLVMVMFSGYENFVSQLDISENKEKLNWLGKMDA
                     TSLKNKVAASIVAISSIHLLRVFMDAKNVPDNKLMWYVIIHLTFVLSAFVMGYLDRLT
                     RHNH"
     gene            13046..13930
                     /gene="yghA"
                     /locus_tag="NBI74_001337"
     CDS             13046..13930
                     /gene="yghA"
                     /locus_tag="NBI74_001337"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HCD1931699.1"
                     /translation="MSHLKDPTTQYYTGEYPKQKQPTPGIQAKMTPVPDCGEKTYVGS
                     GRLKDRKALVTGGDSGIGRAAAIAYAREGADVAISYLPVEEEDAQDVKKIIEECGRKA
                     VLLPGDLSDEKFARSLVHEAHKALGGLDIMALVAGKQVAIPDIADLTSEQFQKTFAIN
                     VFALFWLTQEAIPLLPKGASIITTSSIQAYQPSPHLLDYAATKAAILNYSRGLAKQVA
                     EKGIRVNIVAPGPIWTALQISGGQTQDKIPQFGQQTPMKRAGQPAELAPVYVYLASQE
                     SSYVTAEVHGVCGGEHLG"
     gene            complement(14202..14627)
                     /gene="exbD"
                     /locus_tag="NBI74_001338"
     CDS             complement(14202..14627)
                     /gene="exbD"
                     /locus_tag="NBI74_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein ExbD"
                     /protein_id="HCD1931700.1"
                     /translation="MAMHLNENLDDNGEMHDINVTPFIDVMLVLLIIFMVAAPLATVD
                     VKVNLPASTSTPQPRPEKPVYLSVKADNSMFIGNDPVTDETMITALNALTEGKKDTTI
                     FFRADKTVDYETLMKVMDTLHQAGYLKIGLVGEETAKAK"
     gene            complement(14634..15368)
                     /gene="exbB"
                     /locus_tag="NBI74_001339"
     CDS             complement(14634..15368)
                     /gene="exbB"
                     /locus_tag="NBI74_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HCD1931701.1"
                     /translation="MGNNLMQTDLSVWGMYQHADIVVKCVMIGLILASVVTWAIFFSK
                     SVEFFNQKRRLKREQQLLAEARSLNQANDIAADFGSKSLSLHLLNEAQNELELSEGSD
                     DNEGIKERTSFRLERRVAAVGRQMGRGNGYLATIGAISPFVGLFGTVWGIMNSFIGIA
                     QTQTTNLAVVAPGIAEALLATAIGLVAAIPAVVIYNVFARQIGGFKAMLGDVAAQVLL
                     LQSRDLDLEASAAAHPVRVAQKLRAG"
     gene            15620..16807
                     /gene="metC"
                     /locus_tag="NBI74_001340"
     CDS             15620..16807
                     /gene="metC"
                     /locus_tag="NBI74_001340"
                     /EC_number="4.4.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HCD1931702.1"
                     /translation="MADKKLDTQLVNAGRSKKYTLGAVNSVIQRASSLVFDSVEAKKH
                     ATRNRANGELFYGRRGTLTHFSLQQAMCELEGGAGCALFPCGAAAVANSILAFVEQGD
                     HVLMTNTAYEPSQDFCSKILSKLGVTTSWFDPLIGADIVKHLQPNTKIVFLESPGSIT
                     MEVHDVPAIVAAVRSVVPDAIIMIDNTWAAGVLFKALDFGIDVSIQAATKYLVGHSDA
                     MIGTAVCNARCWEQLRENAYLMGQMVDADTAYITSRGLRTLGVRLRQHHESSLKVAEW
                     LAEHPQVARVNHPALPGSKGHEFWKRDFTGSSGLFSFVLKKKLSNEELANYLDNFSLF
                     SMAYSWGGYESLILANQPEHIAAIRPQGEIDFSGTLIRLHIGLEDVDDLIADLDAGFA
                     RIV"
     gene            16947..17606
                     /locus_tag="NBI74_001341"
     CDS             16947..17606
                     /locus_tag="NBI74_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCD1931703.1"
                     /translation="MAVIQDIIAALWQHDFAALADPHIVSVVYFVMFATLFLENGLLP
                     ASFLPGDSLLILAGALIAQGVMDFLPTIAILTAAASLGCWLSYIQGRWLGNTKTVKGW
                     LAQLPAKYHQRATCMFDRHGLLALLAGRFLAFVRTLLPTMAGISGLPNRRFQFFNWLS
                     GLLWVSVVTSFGYALSMIPFVKRHEDQVMTFLMILPIALLTAGLLGTLFVVIKKKYCN
                     A"
     gene            complement(17646..18545)
                     /gene="yqhC"
                     /locus_tag="NBI74_001342"
     CDS             complement(17646..18545)
                     /gene="yqhC"
                     /locus_tag="NBI74_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001407451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YqhC"
                     /protein_id="HCD1931704.1"
                     /translation="MKREEICRLLADKVNKLKNKENSLSELLPDVRLLYGETPFARTP
                     VMYEPGIIILFSGHKIGYINERVFRYDANEYLLLTVPLPFECETYATSEVPLAGLRLN
                     VDILQLQELLMDIGEDEHFQPSMAASGINSATLSEEILCAAERLLDVMERPLDARILG
                     KQIIREILYYVLTGPCGGALLALVSRQTHFSLISRVLKRIENKYTENLSVEQLAAEAN
                     MSVSAFHHNFKSVTSTSPLQYLKNYRLHKARMMIIHDGMKASAAAMRVGYESASQFSR
                     EFKRYFGVTPGEDAARMRAMQGN"
     gene            18739..19902
                     /gene="yqhD"
                     /locus_tag="NBI74_001343"
     CDS             18739..19902
                     /gene="yqhD"
                     /locus_tag="NBI74_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HCD1931705.1"
                     /translation="MNNFNLHTPTRILFGKGAIAGLREQIPHDARVLITYGGGSVKKT
                     GVLDQVLDALKGMDVLEFGGIEPNPAYETLMNAVKLVREQKVTFLLAVGGGSVLDGTK
                     FIAAAANYPENIDPWHILQTGGKEIKSAIPMGCVLTLPATGSESNAGAVISRKTTGDK
                     QAFHSAHVQPVFAVLDPVYTYTLPSRQVANGVVDAFVHTVEQYVTKPVDAKIQDRFAE
                     GILLTLIEDGPKALKEPENYDVRANVMWAATQALNGLIGAGVPQDWATHMLGHELTAM
                     HGLDHAQTLAIVLPALWNEKRDTKRAKLLQYAERVWNITEGSDDERIDAAIAATRNFF
                     EQLGVPTHLSDYGLDGSSIPALLKKLEEHGMTQLGENHDITLDVSRRIYEAAR"
     gene            20007..20834
                     /gene="dkgA"
                     /locus_tag="NBI74_001344"
     CDS             20007..20834
                     /gene="dkgA"
                     /locus_tag="NBI74_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgA"
                     /protein_id="HCD1931706.1"
                     /translation="MANPTVIKLQDGNVMPQLGLGVWQASNEEVITAIQKALEVGYRS
                     IDTAAAYKNEEGVGKALKNASVNREELFITTKLWNDDHKHPREALLDSLKKLQLDYID
                     LYLMHWPVPAIDHYVEAWKGMIELQKEGLIKSIGVCNFQIHHLQRLIDETGVTPVINQ
                     IELHPLMQQRQLHAWNATHKIQTESWSPLAQGGKGVFDQKVIRDLADKYGKTPAQIVI
                     RWHLDSGLVVIPKSVTPSRIVENFDVWDFRLDKDELGEIAKLDQGKRLGPDPDQFGG"
     gene            21034..21960
                     /locus_tag="NBI74_001345"
     CDS             21034..21960
                     /locus_tag="NBI74_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjP/YqhG family protein"
                     /protein_id="HCD1931707.1"
                     /translation="MKIILLFLSALASFTVHAQPPSQTVEQTVRHIYQNYKSDATAPY
                     FGETGERAITSARIQQALTLNDNLTLPGNIGWLDYDPVCDCQDFGDLVLESVAITQTD
                     ADHADAIVRFRIFQDDKEKTSQTLKMVAENGRWVIDDIVSNHGSVLQAVNSENEKTLA
                     AIASLQKEQPEAFVAELFEHIADYSWPWTWVVSDSYRQAVNAFYKTTFKTANNPDEDM
                     PIERQFIYDNPICFGEESLFSRVDEIRVLEKTTDSARIHVRFTLTNGNNEEQELILQR
                     REGKWEIADFIRPNSGSLLKQIEAKTAARLKQ"
     gene            22011..22268
                     /gene="yqhH"
                     /locus_tag="NBI74_001346"
     CDS             22011..22268
                     /gene="yqhH"
                     /locus_tag="NBI74_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YqhH"
                     /protein_id="HCD1931708.1"
                     /translation="MKTIFTVGAVVLATCLLSGCVNEQKINQLASNVQTLNAKIARLE
                     QDMKALRPQIYAAKSEANRANTRLDAQDYFDCLRCLRMYAE"
     gene            complement(22310..24529)
                     /locus_tag="NBI74_001347"
     CDS             complement(22310..24529)
                     /locus_tag="NBI74_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HCD1931709.1"
                     /translation="MSSISLIQPDRDLFSWPQYWAACFGPAPFLPMSREEMDQLGWDS
                     CDIILVTGDAYVDHPSFGMAICGRMLEAQGFRVGIIAQPDWSSKDDFMRLGKPNLFFG
                     VTAGNMDSMINRYTADRRLRHDDAYTPNNVAGKRPDRATLVYTQRCKEAWKDVPVILG
                     GIEASLRRTAHYDYWSDTVRRSVLVDSKADMLMFGNGERPLVEVAHRLAMGEPISEIR
                     DVRNTAIIVKEALPGWSGVDSTRLDTPGKIDPIPHPYGEDLPCADNKPVAPKKQEAKA
                     VTVQPPRPKPWEKTYVLLPSFEKVKGDKVLYAHASRILHHETNPGCARALMQKHGDRY
                     VWINPPAIPLSTEEMDSVFALPYKRVPHPAYGNARIPAYEMIRFSVNIMRGCFGGCSF
                     CSITEHEGRIIQSRSEDSIINEIEAIRDTVPGFTGVISDLGGPTANMYMLRCKSPRAE
                     QTCRRLSCVYPDICPHMDTNHEPTINLYRRARDLKGIKKILIASGVRYDIAVEDPRYI
                     KELATHHVGGYLKIAPEHTEEGPLSKMMKPGMGSYDRFKELFDTYSKQAGKEQYLIPY
                     FISAHPGTRDEDMVNLALWLKKHRFRLDQVQNFYPSPLANSTTMYYTGKNPLAKIGYK
                     SEDVFVPKGDKQRRLHKALLRYHDPANWPLIRQALEAMGKKHLIGSRRDCLVPAPTIE
                     EMREARRQNRNTRPALTKHTPMATQRQTPATAKKASSTQSRLQNAGAKKRPKAAVGR"
     gene            24781..25530
                     /locus_tag="NBI74_001348"
     CDS             24781..25530
                     /locus_tag="NBI74_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000438650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HCD1931710.1"
                     /translation="MEQVITKRRYYDIGLQIEELLYSGVFKAGERLPSERELSERFNT
                     SRTTIREAIIMLELKGVLNVKQGSGIFFVDSTDKLNQKSLMPYSEIGPFELLQARQVI
                     ESNITGFAASQISFNELQELKKIIGLQEKAIAAESDKFEDLDHRFHSIIAEATQNRVL
                     IKQAAELWRAVRTENPRWKKLNYKYLHEKHLRLQWLEDHRAIFLALQQKDSELAREAS
                     WRHLENSKNELIKIFKQDASISDFDDFFFAR"
     gene            25853..27325
                     /locus_tag="NBI74_001349"
     CDS             25853..27325
                     /locus_tag="NBI74_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HCD1931711.1"
                     /translation="MSGNESIDAAHYDQVTTWPRGGLQADIVHIGFGAFHRGHQAVYT
                     DLTNQLSDTRWGIFEINLFGDAQLIENLNAQNGLFSVVETSASQSTSRLVRSVAGGIH
                     TPRDGIAAAIHKLAEPQVKIVSLTITEKGYCLDPQTRSLDLTNGLIQHDLQNPDAPLS
                     AIGVIVCALQQRKATGLAAFSVLSCDNLPDNGHLTRNAVLGFARQLDQPLAQWIEENV
                     SFPGTMVDRIVPAMTESQFALLETKTGYADPCGIVCESFRQWVIEDNFVRGRPEWDKA
                     SAMFVSNVQPYEEMKLRMLNGSHSFLAYNGSLAGYEFIWQCMEDANFRSITRQLMINE
                     QARTLNPDLNINIQEYADLLIERFSNRNVAHRTGQIAMDGSQKLPQRALTPWLKLHQQ
                     KQNNAVLSLLVAGWLHYVIDAVEKSQSVADPMNDQLQALIKEQQDAWQQALALLHLSA
                     IFGDLSNHQPFINEIKIAFANIKNKGIKATISQLLSDEQK"
     gene            27322..28338
                     /locus_tag="NBI74_001350"
     CDS             27322..28338
                     /locus_tag="NBI74_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000853917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HCD1931712.1"
                     /translation="MKTLICQQPGVMEYVEKDIPTPADNEVLLKIKAVGICGTDIHAF
                     AGRQPFFSYPRVLGHEICAEAVSRGSQCQTAQLGQRYSVIPCIPCGECAACREEKTNC
                     CERVSLYGVHQDGGFSEYLAVREDNLVPLPDEVSDSAGALVECFAIGAHAVRRAEIKA
                     EQNVLVIGAGPIGLATAAIARAKGAHVVVADIDCQRRQHVVDHLAINAFNPTQEDFIA
                     ALREAFGGELACIVLDATGNKASMSHDVNLIRHGGKIVFIGLYIGELVIDDPTFHKKE
                     TTLLSSRNATREDFALVIKLMRSNKIHENLMKNQAFDFFSVGEDYQRNVVENKNMVKG
                     VITF"
     gene            28349..29359
                     /locus_tag="NBI74_001351"
     CDS             28349..29359
                     /locus_tag="NBI74_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000214530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ldh family oxidoreductase"
                     /protein_id="HCD1931713.1"
                     /translation="MTTVYVSEENLKSLVHHKLHTAGLDTDTTQQVTDVLVHADITGV
                     HSHGVMRVEHYCTRLAAGGLNKAPQFSIEQISPSVAILDSDDGMGHSALISATEHAIK
                     LAQQEGLGFVSVKNTSHCGALSYFAEMITNKGLVAIVMTQTDTCVAPHGGAERFLGTN
                     PIAFGFPVENSHPMIVDMATSATAFGKILHAKETGKHIGEGLAIDKNGYGTTDPYKIE
                     NLLPFGQHKGSGIALAIDALTGVLMNANFGNHIVRMYGDYDKMRKLASLVIAIDPKKL
                     GNPVFAKTMAKMVTELHAVKPAPGVEKVLAPNDPQMHYKEKCQQEGIPVPAGIFHYLA
                     EN"
     gene            29432..30415
                     /locus_tag="NBI74_001352"
     CDS             29432..30415
                     /locus_tag="NBI74_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000645949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HCD1931714.1"
                     /translation="MKALRPLTASLMLLTSCLLISNTTLAKTTLKLSHNQDKSHAVHK
                     ALSYLADKTKEYSNGELVIRIYPNATLGNERESLELMNSGALQMVKVNAASLESFAPD
                     YSLFSLPFLFRDRDHYYRVLQSDLGKKILNSSESKGFVGITYYDGGARSFYSNKPITK
                     PEDLAGMKIRVQQSPSAIAMMKALGGVATPMAQGELYTALQQGVVDGGENNTVVYADM
                     RHAEVAKVYSRDEHTMVPDVLIISTDVLNKLGDKERTALLKAADESMMQMKDVIWPAA
                     EKEAYDKMKGMNATVVDVDKSAFKERVKPLYDEFKAKDAQSAKNLELVESM"
     gene            30457..30939
                     /locus_tag="NBI74_001353"
     CDS             30457..30939
                     /locus_tag="NBI74_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001556790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HCD1931715.1"
                     /translation="MRTLTHILNKILAGCCCIILAIMVFCVTWQVIARFIFNAPSTVL
                     DEFTQILFMWMILLGGVYTAGLKKHLAIDLLAQKLPAASVLTLDSFIQIIITVFAVIF
                     MIYGGNIVVEKAQHVGQISPVLKWPMDKVYWVMPVSGLILVWYSVMNIIDNYRKRNFH
                     "
     gene            30950..32254
                     /locus_tag="NBI74_001354"
     CDS             30950..32254
                     /locus_tag="NBI74_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HCD1931716.1"
                     /translation="MDFEYIYPVLILFGSFAVMLAIGVPITFAIGLSSLLSIITALPP
                     DAAISVISQKMTVGLDGFTLLAIPFFVLAGNIMNTGGIARRLVNLAQALVGRLPGSLA
                     HCNILANTLFGAISGSAVASAAAVGGIMSPLQEKEGYDPAFSAAVNIASAPIGLMIPP
                     SNVLIVYSLASGGTSVAALFLAGYLPGILTAVALMFVAALYARRHHYPVAERINYRQF
                     LQVFRESIPSLMLIFIIIGGIIAGVFTPTEASAIAVIYSLALAMIYREITLKKLNDIL
                     LDSVVTSSIVLLLVGCSMGMSWAMTNADVPELINELITRVSDNKWVILFIINIILLIV
                     GTFMDITPAILIFTPIFLPIAQHLGIDPIHFGIIMVFNLTIGLCTPPVGTILFVGCSI
                     GKVSIDRAIKPLLPMFLALFVVMAIICYFPQLSLMLPGLFST"
     gene            complement(32325..33737)
                     /gene="ftsP"
                     /locus_tag="NBI74_001355"
     CDS             complement(32325..33737)
                     /gene="ftsP"
                     /locus_tag="NBI74_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsP"
                     /protein_id="HCD1931717.1"
                     /translation="MSLSRRQFIQASGIALCAGAVPLKASAAGQQQPLPVPPLLESRR
                     GQPLFMTVQRAHWSFTLGTRASVWGINGRYLGPTIRVWKGDDVKLIYSNRLTENVSMT
                     VAGLQVPGPLMGGPARMMSPNADWAPVLPIRQNAATLWYHANTPNRTAQQVYNGLAGM
                     WLVEDEVSKSLPIPNHYGVDDFPVIIQDKRLDNFGTPEYNEPGSGGFVGDTLLVNGVQ
                     SPYVEVSRGWVRLRLLNASNSRRYQLQMSDGRPLHVISGDQGFLPAPVSVKQLSLAPG
                     ERREILVDMSNGDEVSITCGEAASIVDRIRGFFEPSSILVSTLVLTLRPTGLLPLVTD
                     SLPMRLLPTEIMAGSPIRSRDISLGDDPGINGQLWDVNRIDVTAQQGTWERWTVRADE
                     PQAFHIEGVMFQIRNVNGAMPFPEDRGWKDTVWVDGQVELLVYFGQPSWAHFPFYFNS
                     QTLEMADRGSIGQLLVNPVP"
     gene            complement(33812..34549)
                     /gene="plsC"
                     /locus_tag="NBI74_001356"
                     /gene_synonym="parF"
     CDS             complement(33812..34549)
                     /gene="plsC"
                     /locus_tag="NBI74_001356"
                     /gene_synonym="parF"
                     /EC_number="2.3.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acylglycerol-3-phosphate O-acyltransferase"
                     /protein_id="HCD1931718.1"
                     /translation="MLYIFRLIITVIYSILVCVFGSIYCLFSPRNPKHVATFGHMFGR
                     LAPLFGLKVECRKPADAESYGNAIYIANHQNNYDMVTASNIVQPPTVTVGKKSLLWIP
                     FFGQLYWLTGNLLIDRNNRTKAHGTIAEVVNHFKKRRISIWMFPEGTRSRGRGLLPFK
                     TGAFHAAIAAGVPIIPVCVSTTSNKINLNRLHNGLVIVEMLPPIDVSQYGKDQVRELA
                     AHCRSIMEQKIAELDKEVAEREAAGKV"
     gene            complement(34784..37042)
                     /gene="parC"
                     /locus_tag="NBI74_001357"
     CDS             complement(34784..37042)
                     /gene="parC"
                     /locus_tag="NBI74_001357"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HCD1931719.1"
                     /translation="MSDMAERLALHEFTENAYLNYSMYVIMDRALPFIGDGLKPVQRR
                     IVYAMSELGLNASAKFKKSARTVGDVLGKYHPHGDSACYEAMVLMAQPFSYRYPLVDG
                     QGNWGAPDDPKSFAAMRYTESRLSKYSELLLSELGQGTADWVPNFDGTLQEPKMLPAR
                     LPNILLNGTTGIAVGMATDIPPHNLREVAQAAIALIDQPKTTLDQLLDIVQGPDYPTE
                     AEIITSRAEIRKIYENGRGSVRMRAVWKKEDGAVVISALPHQVSGARVLEQIAAQMRN
                     KKLPMVDDLRDESDHENPTRLVIVPRSNRVDMDQVMNHLFATTDLEKSYRINLNMIGL
                     DGRPAVKNLLEILSEWLVFRRDTVRRRLNYRLEKVLKRLHILEGLLVAFLNIDEVIEI
                     IRNEDEPKPALMSRFGLTETQAEAILELKLRHLAKLEEMKIRGEQSELEKERDQLQGI
                     LASERKMNNLLKKELQADAQAYGDERRSPLQEREEAKAMSEHDMLPSEPVTIVLSQMG
                     WVRSAKGHDIDAPGLNYKAGDSFKAAVKGKSNQPVVFVDSTGRSYAIDPITLPSARGQ
                     GEPLTGKLTLPPGATVDHMLMESDDQKLLMASDAGYGFVCTFNDLVARNRAGKALITL
                     PENAHVMPPVVIEDASDMLLAITQAGRMLMFPVSDLPQLSKGKGNKIINIPSAEAARG
                     EDGLAQLYVLPPQSTLTIHVGKRKIKLRPEELQKVAGERGRRGTLMRGLQRIDRVEID
                     SPRRASSGDSEE"
     gene            complement(37180..38787)
                     /locus_tag="NBI74_001358"
     CDS             complement(37180..38787)
                     /locus_tag="NBI74_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCD1931720.1"
                     /translation="MYTRNLLWLVSLVSAAPLYAADVPANTPLAPQQVFRYNNHSDPG
                     TLDPQKVEENTAAQIVLDLFEGLVWMDGEGQVQPAQAERWEILDDGKRYIFHLRSGLQ
                     WSDGQPLTAEDFVLGWQRAVDPKTASPFAGYLAQAHINNAAAIVAGKADVTSLGVKAT
                     DDRTLEVTLEQPVPWFTTMLAWPTLFPVPHHVIAKHGDSWSKPENMVYNGAFVLDKWV
                     VNEKITARKNPKYRDAQHTVLQQVEYLALDNSVTGYNRYRAGEVDLTWVPAQQIPAIE
                     KSLPGELRIIPRLNSEYYNFNLEKPPFNDVRVRRALYLTVDRQLIAQKVLGLRTPATT
                     LTPPEVKGFSATTFDELQKPMSERVAMAKVLLKQAGYDASHPLRFELFYNKYDLHEKT
                     AIALSSEWKKWLGAQVTLRTMEWKTYLDARRAGDFMLSRQSWDATYNDASSFLNTLKS
                     DSEENVGHWKNAQYDALLNQATQITDATKRNALYQQAEVIINQQAPLIPIYYQPLIKL
                     LKPYVGGFPLHNPQDYVYSKELYIKAH"
     gene            complement(38896..39378)
                     /locus_tag="NBI74_001359"
     CDS             complement(38896..39378)
                     /locus_tag="NBI74_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HCD1931721.1"
                     /translation="MTNLTLDVNIIDFPSIPVAMLPHRCSPELLNYSVAKFIMWRKET
                     GLSPVNQSQTFGVAWDDPATTAPEAFRFDICGSVSEPIPDNRYGVSNGELTGGRYAVA
                     RHVGELDDISHTIWGIIRHWLPASGEKMRKAPILFHYTNLAEGMTERRLETDIYVPLA
                     "
     gene            complement(39431..39823)
                     /gene="ygiW"
                     /locus_tag="NBI74_001360"
     CDS             complement(39431..39823)
                     /gene="ygiW"
                     /locus_tag="NBI74_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB fold stress tolerance protein YgiW"
                     /protein_id="HCD1931722.1"
                     /translation="MKKFAAVIAVMALCSAPVMAAEQGGFSGPSATQSQAGGFQGPNG
                     SVTTVESAKSLRDDTWVTLRGNIVERISDDLYVFKDASGTINVDIDHKRWNGVTVTPK
                     DTVEIQGEVDKDWNSVEIDVKQIRKVNP"
     gene            39975..40634
                     /gene="qseB"
                     /locus_tag="NBI74_001361"
     CDS             39975..40634
                     /gene="qseB"
                     /locus_tag="NBI74_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator QseB"
                     /protein_id="HCD1931723.1"
                     /translation="MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAP
                     YDAVILDLTLPGMDGRDILREWRGKGQREPVLILTARDALAERVEGLRLGADDYLCKP
                     FALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
                     LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGE
                     K"
     gene            40631..41980
                     /gene="qseC"
                     /locus_tag="NBI74_001362"
     CDS             40631..41980
                     /gene="qseC"
                     /locus_tag="NBI74_001362"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     QseC"
                     /protein_id="HCD1931724.1"
                     /translation="MKFTQRLSLRGRLTLIFLILASVTWLLSSFVAWKQTTDNVDELF
                     DTQLMLFAKRLSTLDLNEINAADRMAQTPNKLKHGHVDDDALTFAIFTHDGRMVLNDG
                     DNGEDIPYSYQREGFADGQLVGEDDPWRFVWMTSPDGKYRIVVGQEWEYREDMALAIV
                     AGQLIPWLVALPIMLIIMMVLLGRELAPLNKLALALRMRDPDSEKPLNATGVPSEVRP
                     LVESLNQLFARTHTMMVRERRFTSDAAHELRSPLTALKVQTEVAQLSDDDPQARKKAL
                     LQLHSGIDRATRLVDQLLTLSRLDSLDNLQDVAEIPLEDLLQSSVMDIYHTAQQAKID
                     VRLTLKANGIKRTGQPLLLSLLVRNLLDNAVRYSPQGSVVDVTLNADNFIVRDNGPGV
                     TPEALARIGERFYRPPGQTATGSGLGLSIVQRIAKLHDMNVEFGNAKQGGFEAKVSW"
     gene            complement(42026..>42289)
                     /locus_tag="NBI74_001363"
     CDS             complement(42026..>42289)
                     /locus_tag="NBI74_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2645 family protein"
                     /protein_id="HCD1931725.1"
                     /translation="DSFFAFNKYDWMLESGDNICSIPSVSGEDRILQAMIAAFFLLTP
                     LIILILRKLFMREMFEFWLYVFSLVICLGCGWWLFWGRFIFCY"
BASE COUNT        10579 a        10974 c        10499 g        10237 t
ORIGIN      
        1 tactgactat tcttcataat cagtacggga ttgctacaac atgtattact ctttcaccac
       61 aattaacggt tcaatatcac tctctttttt aataaccagt gattcatcac cgcgcaaaca
      121 cgtcccaccg tagttgccgc caacggtgaa ggtacatacc tgaatgtatt taccgtccac
      181 tttcggcaaa caccacagtt gctgatagat gtttttctgc tcggcaaatt taccgctggt
      241 tttgtccagc acctcttcat gatggctgac caggtcgata ttgctgccac agcgaccggc
      301 aatcggtttc actgcgtaac ctgttttcac cagttcatca ttaacagtga aatcggtatc
      361 cagcaggtaa cgatggtgcg ggaacagcga ccagaggatc ggcagaatcg ctttgttgcc
      421 ggggatcacc gtccacagcg gctcaaagac cagcacttcc gggcgcagca atacgtcgat
      481 aagacgcact tcgttttgcg gatgaccggt acggattggc accgcagcaa actcacggtc
      541 gctaacttca cgaatctgat caaacgcggt ttcccacgcc caggttttcc acacgcagtt
      601 aaccagccgt ccttccccat caatcagttg cccggcagca tcccagccca gttcatccag
      661 cccgcgcaag atacgcgttt caaagcccgc caggtgcagc gcctgctcca taaactgcgc
      721 gtgatagttt tcctcgatat ctttgtcctg catgatatgg acaaacggac gtgcacgact
      781 gtgtttccag gcaccagcca gttcgttaat cagcccttcc gccggattga agccgttgcc
      841 tttatagccc tgctccgccc aacgttcgag gatcaagccc gcttcggtat gacaggaggc
      901 ggagtcggcg ttgtattcgt aaaccttcag gccacgctca tccatgcaga aatccatacg
      961 accagtgatc atatggtgac ggcgacgctg ccaggagaga cgcaaacgtg gccagaggat
     1021 tttcgggatg tcgaacagcg ccagcaggtt gtcatctttc agcaccttgt cggttgcgtg
     1081 aagatacatc aggtgcagct cgttggtggc tttaattagc tcctgctcgg cactctcggt
     1141 aatggtgtag taatgatacg gatcctgatt gatcacctga ccgttggcct gcacataggc
     1201 gttttgtagc ggatcttttt catccagcca tttaccgtca aactggcctt tgttttccag
     1261 gcgcgctccg ctgattttca gcagctcgcc tgcaatttcc ggctgcggta agctgtattc
     1321 agtatcttcc gtctggatca tccagcccag aatggtggtg tcatcaaaag tgtctttcag
     1381 ggtatagcag ccgttttcga ccaccatctc cagctcacgc gtccactgtt gcccttgcgg
     1441 caacggggaa tgaatcacgt tctgttccgc aatacggact ttgttgccat gcaattgggt
     1501 aatgatggcg acatggccag tgtctttaaa ttcaccgcct ttatcccaga taagaagcgc
     1561 acccgcgacc ggcgcacgcg gcgagccgtt aggaaatgcc tgcaatggca ggatgttgtc
     1621 attaaccact tctcgcagga agcgcagcga gaaaatctcc cacgccatac ccacgtcagt
     1681 aaagaccaca ccgtaattca gaaagagaaa acggcgagca aattcaacgc attgccactt
     1741 gtggcccata tattcgtcgt cgatatagct acggaatacg gcgtcatctt cgtattcctg
     1801 cggatcgaga gaactgtaat ctgaagagta gattgctacc ccacctgggg cgtagcccaa
     1861 taatgtcccg aacggggcat cctggctggt cgttcctttg ctcatcactt tacctttaac
     1921 aatacaacct aagcagttgg cgtaattgtt gtgctctgat tacctgctca gcgacattaa
     1981 ccggacagag gtcagactaa tcatacacct catcgcagcg gctggcgcaa agggttaaaa
     2041 aattcacatt ctgtacagca agtgacctgc tacactgctt caacactacc actcagaagg
     2101 caactcacta tgacagacaa tacttatcag cccgcgaaag tctggacgtg ggataaatcc
     2161 gctggcggcg cgttcgccaa tatcaatcgc ccggtttctg gtccgacgca tgaaaaaacg
     2221 ctgccggtgg gcaaacatcc attacaactt tattcgctgg gaacgccgaa cggtcagaaa
     2281 gtaacgatta tgcttgagga gctgctggcg ctgggcgtta ctggtgcaga gtacgacgcc
     2341 tggctgattc gtattggcga tggcgatcaa ttctccagcg gctttgtcga agtgaaccca
     2401 aactcgaaga tcccggcgct gcgcgatcat acgcataatc cgccgatccg cgtgtttgaa
     2461 tctggttcga tcctgcttta tctggcggag aaatttggct acttcctgcc gcaggatttg
     2521 gcaaagcgta ctgaaacgat gaactggcta ttctggttac agggcgcggc accgttcctc
     2581 ggcggtggtt ttggtcactt ttaccattac gcaccggtaa agattgagta cgccatcaac
     2641 cgctttacca tggaagccaa acgtctgctc gacgtgctgg ataagcaact ggcgcagcat
     2701 aagtttgttg cgggcgatga gtacaccatt gcggatatgg cgatttggcc gtggtttggc
     2761 aacgtggtgt taggtggtgt gtatgatgcc gctgagtttc ttgatgcggg cagttataag
     2821 catgtacaac gctgggcgaa agaagtaggc gaacgtccgg cggtgaaacg tgggcgtatt
     2881 gttaaccgca ccaacggacc gctgaatgag cagttgcatg agcgccatga cgccagtgat
     2941 ttcgagacga atacggaaga taagcgtcag gggtaagggt tggtgttcgt cgcagcaagc
     3001 catccaggcc ggataaggcg ttcacgccgc atccggcaat cgtgcacaat gcctgatgcg
     3061 acgctgccgc gtcttatcag gcctacgaaa gctaatcatc acgcgctggt aatgtcgtaa
     3121 tccatttggc gcagtgcgtc taatgtggct ttggcttcat cttcgtcgat gatgctcatg
     3181 gcaaatccga cgtgtaccag cacccactgg cccagtagat cggcagggtt accttcacaa
     3241 atcagggcga tattcacatc gcgcttgata ccacatactt caacctgcgc aagctggtga
     3301 atatcttcac cgacagccag cacctggcct ggaacgccaa tacacattat taactccggt
     3361 tattcaactt caatactttt gacgatcagc gaatcgccgg tatcgacacg caaccgctcg
     3421 ccgtgacaga gcggacactg cgcatcgtgc tgatgaatct ccaccacctg gctgcaatcc
     3481 cagcaccagg cctgggcggg tttatagacg atatgtaaat cgcacccttg cgccaccgtt
     3541 ccgtggcaga caatttcaaa actaaaacgg acggcgctct cctcaacgca ggagagcgcg
     3601 ccaatttcca gccacacggc ggtgacgcgc ttaacatcgt gctgctccgc ctgccgttgg
     3661 ataatttcaa cggcgctctg gcaaagagac aactcatgca ttttcgccac tcctgcgacc
     3721 aaacagcagg gcgcgacgcc ctgcgtgtgg tacatccgga ttcgtgactg gcagcgaaag
     3781 gatcattcgt gcgcagtcat ccgtcaggcg ttggccctct tcaatcgaca tgctgtgcga
     3841 aagcggcgac atcagcgaac aggagagata ttgcgaaaca ccgtccagtt cgccaacggt
     3901 aaaggtcata gtgccatacg gcagttgcag accaattttt tcactcactt tgcgcagcgg
     3961 ccagagttga tccgggccgg ggaagataac tgcactcagc atccacgggg tgatcacaca
     4021 ccccgtccac tgaccttcga acagcgtaaa atcagaaaca tacaccggca ttgaaggatg
     4081 cagaaaagag agatcgtgca tcgaacgccg ggcaatttct tcaaacgctg cctgtacttg
     4141 cgccttcggg gaggtctgga aacctgctat ctcttcagtc atgaatcgcc tcccgtggga
     4201 tggcttccac gcctgattcg cgcagcgcag ccagaatctg ctcaagcgca ggttcaatca
     4261 ttgcttcaac cgtcggcgtt aagccgatgt gtggctccag cgattccggg atcacgccga
     4321 ccagggtcag ctttttcgga aactcaccgg tgaagcgcag ggccgacaat acgtcggcca
     4381 ggccaagctg atgcggagag attttgttgg taaacaacgc cggaacttct tcatcccgca
     4441 ggatcatcat cgttcccggc gtgttctttt tcgacacaat ggcatccgca ataatcagat
     4501 gatcgcgatt tgccatgtcg ccaagcagct ccattcccgc cgtgccgcca tcgaggatct
     4561 caacataatc cggcagaatg tatcgttgct ctaacgcttc gacaatccgc acaccgatgg
     4621 cttcatcggt cagcaaaata ttgccgaccc ctaagactaa aatacgcatt acagaacctt
     4681 cactgaaacc acttcgttgc cgtcggcatc cactacgtgt accgcacagg ccatgcacgg
     4741 gtcgaaggag tgaatggtac gcaccacttc cagcggttta ttcggatcag caaccggtgt
     4801 acccaccagc gactgctcgt aaggaccgac gtcatcattg aagttacgcg gaccagagtt
     4861 ccaggttgat ggaacaactg cctggtagtt actgataata ccgtctttaa taaccatcca
     4921 gtgagagagc ataccgcgcg gcgcttcgag gaagccaaca cctttgaact cacccgttgc
     4981 cggaatgttc ggtttcacaa aggtggtgtg atcgcctttg ccgatattgg tgatcagtgc
     5041 actgtattga ttttgcagga tatcctgcaa ttcgcagcag tgaacggtac gaccaataat
     5101 acggcccagc gtggagtgca gctgcgccac ttccagcgtg ttgccagtca gtttctgata
     5161 aatcgcaacg atttcattca gtttgttctg ggtagattcg cgacctgccg ccagtttcac
     5221 cagcatatta gccagcggac ccacttctac cgttttgccg tagaaagtcg gtgatttcac
     5281 ccaggaatat ttcccgtcgt cagaccaacc atcataagcc ggaatggtgg taccttccca
     5341 cggtgcctgc ggcgcttcgt ctttatacca ggagtgcttc gcgctttcct gaatgccttt
     5401 gatcaggtat tcatcggaat gagaagtgat cggacgatac gaggacagat ccgcattctc
     5461 aatgtagccg cccgggaaca ggaagctgcc gtttttactg tcggtcggga attccggcac
     5521 gctcaggtag ttcaccgcac ctttaccgcg cgtcagccat tccgggtaga acgcggcaat
     5581 aactgcggta tcaaccttat aaacctgttc aacaaagtcg ctcagcttgt cgatgaaaga
     5641 cttgatgtac atcaggcgct caaggttcag cacgcccaaa ccgtcgaggt tgatcgggtt
     5701 cgcgacacca cctaccgcca ggttctgaat gtgcggcgtt ttaccgccca gcagcgccac
     5761 gacgcggtta gcgtcacgct ggcactccag cgcctgcagg tagtgcgcta ccgcaatcag
     5821 gttcacttcc ggcggcagtt tcattgccgg gtgaccccag tagccgttgg cgaaaatccc
     5881 caactgaccg ctggcaacca gatctttgat cttgttctga actttggtga actcttccgg
     5941 gctgttcaga tgccaggtcg aaacgccttt cagcatttcg gaggctttgg ttgggtcagc
     6001 ttgcagtgca gaagtgatat ccacccagtc cagcgccgaa agctgataga aatgaacgat
     6061 atggtcatgc gtggtgtgcg cagccagaat gatgttacgg atgtattgcg cgttaaccgg
     6121 aacgtcgata ttcagcgcac tttctgccgc acgaacggaa gacagcgcgt gagtggtagt
     6181 acatacgcca cagatacgtt gcacaatcat ccatgcatcg cgcggatcgc ggtttttcac
     6241 gatctcttcc atgccgcgcc acatggtacc ggaagcccat gctttcgaaa cgacgccatt
     6301 ttcgatttcg caatcgatgc gtaaatgccc ctcaatacgg gttaccggat caatagtaat
     6361 tctctggctc atgctttgct cgcctcatga cgattatgat cgttttgttt taaaggagga
     6421 agtatcggca gtagacgaat gagtacgatg taagcgcaaa tctcaatagc cacaaaacca
     6481 atagaaatca acagttcttc ccaggtcggg aagtaggcgt aaccgccgcc cgggttgaat
     6541 gccaccagcg aataggtcag acgccaggtt gcacaaccta acagtgcgct cagtgctgac
     6601 aggaacagca tgcgggaatc attacgcagc ttcgccacac gcagaacgac cagcgggaag
     6661 agcatcagca ggacttcaat ccagaacatc acggagtaga agtcaccggc aaacgctaac
     6721 gacagcttgt cgcgatagat aagctcgcca aagcgcagca cgatgaaaat cgccagcaac
     6781 acactgatgg tgttggtcag cttgacgaac agactctttt catccggacc gttgccacgc
     6841 agacccgcct gcaccagcga accttcaaag atgacaatcg agaagcccat gatgaacgcc
     6901 gtcagcagcg agaacagcgg caacatttca tagctctgcc acaacggatg caccttgtag
     6961 cccgccgaga tcatcagtga ccccattgaa gactggtgca tggtcggcaa cagcgcgcca
     7021 agtgcaatga tgaagaacat caccttgttc agacgcttga gagaaacctt ccagcccaga
     7081 cgttcaaaca gtgccggagc aaactccagt gccatcacgc cgatatagat ggtcatacaa
     7141 accgccgtct cgaacagtac cgagttcacg ttgaagtgac ccggaatgta gaagtacggc
     7201 aggttccagt agcgacccac gtcgatagtg atcgacaagc cacccagtga gtaaccaaac
     7261 agactcgcca acagcgccgg acgcaccagc ggatggtatt gcccacggtt aaagacgtat
     7321 accgcccacg ccagcgccca gccgccacag gcaaagccgg taccaatcag caggtcaaac
     7381 gcgatccaca cgccccacgg gaagccgccg ttcaggtcag agacagagcc cagaccgaac
     7441 accagacgct tcacaatcag gagcatacag atgacgatta acggtccaaa aatcatgacc
     7501 ggtttactga tgattttgcc gcccagcggt tgtggatcat gactcatgat cgtctcctcc
     7561 gtcgtgatgg tcgtttttgg tgttgcgacg aaccagcacg gttaagcccg ccagcacagc
     7621 cagtggtagc atcatgcctt tatacagggt gtgttgaata ttttcggaac gcgcaccggt
     7681 agaaagatcg tccagtttcg gcaggtcgag attttcataa ggcacacccg tcagtaccag
     7741 aacctgagta ccgccgccct ctttctcgcc gtacagatgc ggataatatt tcggcaccgt
     7801 atgcaggtaa gtgtcgccag atttcagcgt ctgacgcgga tagtggtatt cgctgccagg
     7861 cttcagcgcc agacgttttt tcgcctccgc catcagctct tcacgcgtac cgaaaatcac
     7921 cgcgcccgcc gggcacactt ctacgcagcc aggcagaccg cctttatcga gacgttccac
     7981 acctttctgg ttgcacagct cgcacttatg cagcgcacca aacgggttgt tgtagtcgta
     8041 cttcggcacg ttgtacggac aggcgaccat gcagtaacgg cagccggtgc acacgtcttt
     8101 gtcgtaatgg acaatgccgg ttttcggatc ttttttcagt gcagagaccg ggcacacaga
     8161 gacacagttc ggatcgacgc agtgcataca ctgtttctta atgtacgcgt agccgttctc
     8221 ctcctggtct ttgttgaccc ctgtgccgct ggtccacacc tgaatgatgt tattggtata
     8281 cggcgacagt ttgtcgttgt tcgaccaggt ctgttcccct tgcgggttac gttcagggaa
     8341 gttgatatcc tgacacttgg tgacgcaagc ctggcagcct acgcacaagg tcgagtcgta
     8401 caacatcccc agcgatcccg gaattggcgg gcggttttca gcagccgcat gactgacaga
     8461 cggcagcgcg cccgtcagca atgccccgca ggaggctgct ttaataaaat tacgtctgtt
     8521 cacggttatt ctccccgtga gtcagcgtta tctttctttt gctgacgacc cagttcacgc
     8581 accgccatca cgctgacacc ggcaacgagc ccaaccacac cgccgagcaa accaatagcg
     8641 cctgcagaga cgttgccgcc ctctttagcg ttaacatccg gtttctgtga acgcggagtc
     8701 tgattttcga cgttggcaag ctgatggatg cctttatgga agccgatacc ttcttcgtta
     8761 cagccatagc acgggtgacc aatcgccacc ggccacacac cgccaacatc gcagaattgc
     8821 agcgttgagc agttgccata agtttctggc cctttacagc cgaggtggta caggcaccag
     8881 ccttcgcggt ggccttcatc accgaactct ttggcaaaac gaccagcatc gaagtgcggg
     8941 cgacgttcgc agtgttcgtg aatcagacgg ccataggcga aggtcggacg gtttttgtca
     9001 tccagtttcg gcggtttgcc gtaagtgatg atgtgcgcaa cggtcgcgag gaagttgtgc
     9061 gggttcggcg ggcagcccgg aatattgata acggttttgc ctggcagaac ttcttgcagg
     9121 ctgactgcgc cagttgggtt aactccagct gcggcaacac cgccccacgc agagcaggaa
     9181 ccgatagcga taatggctgc tgcgccttcc gccgctttgc ggatgtgatc cacaataggc
     9241 tcaccggcaa ccatgcaata aataccgtta tcttttaatg ggatggaacc atccaccact
     9301 aacacatact gccctttgta cttctcgaga gcgttatgtt tgttctcttc gacctgatga
     9361 ccgaaggcgg cggaaagcac ttcgtgatac tccagagaga tagtctccag tacgaggttt
     9421 tctaccgttg gatgcgttgc acgaagcaga gattccgtac aaccggtgca ctcctgcgcg
     9481 ccaatccaga taactggcgg acgctgcggg ttagtaaccg attcggccat ctctgcagcg
     9541 gctttgctac ttaaccccat ggtggcggct aatgctgcac aaagcttcat gaaatcacga
     9601 cggttaatgc cgtgagaatg gatgagggtg ttatctccag tcatttatag ttattccgtt
     9661 gcgaagacct ggcatatatt ttgcctcaat cgcaaaatca ataatgcgat cgatgcgcca
     9721 tttaccacac atttattatg gttatcgata tcatgatact gcggcggggc gctaaaacga
     9781 agggaagaag cattagtgta attaattaaa gcaagataat acgtatgttt gatcaatttt
     9841 cgaggcgata accggccata aaaaagcgca acgtagaaca ggaattattc tcacgaaaga
     9901 aatccattac catctcttta tccagtccat agcggcgcgt cagaataccc gcttcccagg
     9961 cgctaagctg gcgatctccg gtcttttcgt acatccggtg tgagtagcct aatacaaaac
    10021 cgcgtttata atcggcacag aattttgtca cattaaccgc actgtcggca tgcgttgctt
    10081 ttaatcccgc cattaagcct ttaccaaaat ggttattcat cgttcacctc attcgctata
    10141 tattgtcata tatagcgata ttttaggcaa agaacgactt ttgcgcacgc tattttttca
    10201 tgcttgttta agacgtaatc ccgtaagaga taaacctatc aggagagata tttatcgaac
    10261 catttcagtg tcctttgcca ggcaagatcg gcggcagatt tgtcataacg gggcgtggaa
    10321 tcattatgga atccgtgatt aacccccgga tagatatacg cctcataaac cttattattg
    10381 gctttcaacg ccgcctcgta agcaggccag ccctcgttga ttcgggtgtc cagttccgcg
    10441 aagtggagta gtaaaggcgc ttcaatcttc gccacatcgg cagtgggtgc ctgacgacca
    10501 taaaacggca ccgcgcaggc cagttccgga tacgcgacag ccgccgcgtt cgatacgcca
    10561 ccgccatagc aaaatccggt aatacccact ttgcctgtcg cttgcggata gcgttgcata
    10621 aactcaattg cggcaaagaa atcattcatc agtttggttg gatcaacctg ctgttgcagc
    10681 tcacgacctt tatcatcatt tcccggataa cctccaacgg aacttaagcc gtcaggtgcc
    10741 agggcgatat accccgcctt cgccactcgc cgtgccacat cttcgatata cggattcagt
    10801 ccacgattct catgcaccac cactacggct ggcgttttgc cgctcatctt tgcgggcttc
    10861 accagataac cccgcacctc gccgtgacca tttggcgaag gatacgtgat gtactcagca
    10921 acaatttccg ggtcggtaaa ctctacctga gtcgccagcg cataatttgg cttgagcaaa
    10981 tcaaacaacg ctaatgccgt catcccgcct accgcacttc gccgcaaggt tgaggaactc
    11041 acgtttcgag attttcccgt gagcgtaata gtcatagtaa tccagcaact cttgtgggaa
    11101 atctttggcg gttaaacgcg gcatcgttgc actcctcagt tggtgttttt ttaagcaaag
    11161 cataagcacg tgtttttgcc cagtttttcg tcactctgtg agccagacta cgggatacgc
    11221 gctggcgaat cgctaaacta gaaacattgt ttcgaaattg aacggtggaa aggagaggtc
    11281 atggtctggt tagcgaatcc cgaacgttac gggcagatgc aataccgcta ttgcggaaaa
    11341 agtggtttac gcctgcccgc gttatcgctc ggtttatggc acaatttcgg tcacgttaac
    11401 gcgctggaat cacagcgtgc gatcctgcgt aaagcgtttg atttgggcat tacgcacttt
    11461 gatttagcca acaattacgg gccgcctcca ggaagcgcag aagagaactt tggtcgcctg
    11521 ctgcgggagg attttgccgc ttatcgcgat gaactgatta tctctaccaa ggctggctac
    11581 gatatgtggc ccggccctta cggctctggc ggttcacgta aatacctgct cgccagcctc
    11641 gaccaaagcc tgaagcgtat ggggcttgag tatgtcgata tcttttactc tcatcgcgtc
    11701 gatgaaaata cgccgatgga agaaaccgcc tctgcgctgg ctcatgcggt acaaagcggt
    11761 aaggcgctgt atgtcgggat ctcctcttac tcgccagagc ggacgcaaaa aatggtcgag
    11821 ttgctgcgcg agtggaaaat tccgctgtta attcatcaac cttcgtacaa tttactgaac
    11881 cgctgggtgg ataaaagcgg cctgctggat accctgcaaa ataacggcgt gggctgtatt
    11941 gcctttactc ctctggctca gggattgctg accggaaaat atctcaacgg cattccgcaa
    12001 gattcacgga tgcatcgtga aggaaataaa gttcgtggtc tgacaccgaa aatgcttacc
    12061 gaagccaacc tcaacagcct gcgcttattg aatgaaatgg cacagcagcg tggacaatca
    12121 atggcgcaaa tggcgttaag ctggttgctg aaagatgatc gcgtgacgtc ggtattgatt
    12181 ggtgccagcc gcgcggagca actagaggag aacgtgcagg cgctgaataa tctgacattt
    12241 agcaccaagg agctggcgca gattgatcag catatcgccg atggcgagct gaatctgtgg
    12301 caggcgtctt ccgataaatg acctgttaat aacgggcgcg cgaaccgcgc ccgaatcaga
    12361 tcagtgatta tgacgagtca gtcggtcaag atagcccatc acaaatgcag aaagcacaaa
    12421 cgtcagatgg ataatgacgt accacatcag tttgttatcc gggacatttt tcgcatccat
    12481 aaagacgcgc agtaagtgaa tggaagaaat tgccacaatc gacgctgcta ctttgttttt
    12541 cagcgacgtt gcgtccattt tccccagcca gttcagcttc tctttgttct cggagatatc
    12601 cagctgtgag acgaaattct cataaccgga aaacatcacc atcaccagca aaccgccaac
    12661 cagcgtcata tccaccagcg acagcaacac gaggatcaaa tctgattccg ccatcgagaa
    12721 gatattcggc agtacgtgaa taatctcctg gaagaacttc agcgccaggg caactaacgc
    12781 cagcgaaagg ccaaagtaca cgggggcaag cagccagcga gaagcataca ttgcattttc
    12841 aagaaaacgt tccatacatt cctgtcgtgt agttaaaacg ggcaacagta tatcgcaatt
    12901 gagtaaacag cacgcaacag caaatcaaca tatacgttaa acctttgtcg cataattctg
    12961 aggtttaggg atcttcctgg ttcccttatc aacgcatcga gaacgtctac ccttaaagga
    13021 cgttgaacct gaagggagaa aaacgatgtc tcatttaaaa gacccgacca cgcagtatta
    13081 cactggtgaa tatcccaaac agaaacaacc gacgccaggc atccaggcga agatgacacc
    13141 ggtaccggat tgcggcgaga aaacctatgt tggtagcggt cgcctgaaag atcgtaaagc
    13201 actggtgaca gggggcgatt ccggaatagg tcgcgctgcc gccatcgctt acgcgcgtga
    13261 aggggctgac gtggcgatca gttatcttcc cgtggaagaa gaagacgctc aggatgtgaa
    13321 aaagatcatt gaagaatgcg gacgcaaagc cgttctgctg ccaggcgatt taagcgatga
    13381 gaaatttgcc cgttcgctgg ttcacgaagc gcacaaggcg ttaggcgggc tggatattat
    13441 ggcgctggtc gccgggaaac aggttgccat tccggatatt gcagacctca ccagcgaaca
    13501 gtttcaaaag acctttgcca ttaacgtttt cgcgctgttc tggctaaccc aggaagcgat
    13561 ccccctgcta ccgaaaggtg caagtatcat caccacttcg tcaatccagg cataccagcc
    13621 aagtccgcat ttactggact atgcggctac gaaggcggcg attctgaact acagccgtgg
    13681 cttggcaaaa caggtcgcgg agaaaggtat tcgggtgaat attgtcgcgc caggcccgat
    13741 ctggacagca ctgcaaattt ccggcggaca aacgcaggat aagatcccgc agtttggtca
    13801 gcaaacgccg atgaaacgtg cggggcaacc ggcggaactg gcccctgtat atgtttatct
    13861 ggcaagtcag gagtcgagct acgtcaccgc agaagtgcac ggcgtgtgcg gcggcgagca
    13921 tttaggttaa aaaaatgccc ggttgtgaaa agcaaccggg catcattgtg aattcactct
    13981 tacctgatgt ggcgaaaacg ccttatccgc ctacaaacgc atgaagattc aacatgttgc
    14041 aatagatttt gtaggcctga taagcgtagc gcatcaggca ttgagcacct aatgccggat
    14101 gcgtcgtgaa cgccttatcc ggcctacaaa cgcacgaaga ttcaacatgt tgcaatagat
    14161 tttgtaggcc tgatgagcgt agcgcatcag gcaattctac tttacttcgc tttggcggtt
    14221 tcttcgccga ccagacctat cttcaggtaa cccgcctgat gcagcgtatc cattaccttc
    14281 atcaacgtct cgtaatcgac ggttttgtcc gctcggaaga agatggtggt gtctttcttg
    14341 ccttcggtta acgcattcaa cgccgtaatc attgtttcat cggtgaccgg atcgttaccg
    14401 ataaacatcg agttgtctgc cttcaccgac agataaaccg gtttttccgg gcgcggctgc
    14461 ggcgtgctgg tagaagcagg caagttcacc ttcacatcta ccgtcgctaa cggtgccgcc
    14521 accataaaga taatcagcag aaccaacatc acgtcgataa acggcgtcac gttgatatca
    14581 tgcatttcgc cgttatcgtc gaggttttcg ttaagatgca ttgccatcgg atattatcct
    14641 gcgcgtaatt tttgtgcgac acgaaccgga tgcgcagcgg cgctggcttc cagatccagg
    14701 tcacggcttt gcagcaacaa tacctgcgct gcaacatcac ccagcatcgc tttaaagccg
    14761 ccaatctggc gtgcaaatac gttatagata acgaccgcag gaatcgctgc cacgaggccg
    14821 attgccgttg ctaacagagc ttctgcgata cccggcgcaa cgactgccag gttagtggtc
    14881 tgcgtttgcg cgataccaat aaagctgttc atgatgcccc agaccgtacc aaacagacca
    14941 acgaacggag aaatcgcgcc gatggttgcc agatagccgt taccgcggcc catttgacga
    15001 cccactgcgg cgacccgacg ctccagacgg aagctggtac gttctttaat accttcgtta
    15061 tcgtcgctgc cttctgacag ttccagctca ttctgcgctt cattgagcaa atgcaggctt
    15121 aagcttttgc taccaaaatc agcggcgata tcgttggcct ggtttaagga acgcgcttca
    15181 gccagcagtt gctgctcgcg cttaaggcga cgcttctgat tgaagaattc tacgctctta
    15241 ctgaagaaga ttgcccaagt gactacggag gccaaaataa gcccaatcat cacgcactta
    15301 acgacaatat cggcgtgctg atacataccc cagacggaaa ggtccgtctg catcaaatta
    15361 ttacccactg tgtatctcca ggacgcaagt cacaaaatct gcgcataata atatcaaaat
    15421 gacgtcgaat tgatagtcgt tctcattact atttgcatac tgccgtacct ttgctttctt
    15481 ttccttgcgt ttacgcagta aaaaagtcac cagcacgcca tttgcgaaaa ttttctgctt
    15541 tatgccaatt cttcaggatg cgcccgcgaa tattcatgct agtttagaca tccagacgta
    15601 taaaaacagg aatcccgaca tggcggacaa aaagcttgat actcaactgg tgaatgcagg
    15661 acgcagcaaa aaatacactc tcggcgcggt aaatagcgtg attcagcgcg cttcttcgct
    15721 ggtctttgac agtgtggaag ccaaaaagca cgcgacgcgc aatcgcgcca atggcgagtt
    15781 gttctatgga cggcgcggaa cgttaaccca tttctcctta caacaagcga tgtgtgaact
    15841 ggaaggtggc gcaggctgcg cgctatttcc ctgcggggcg gcagcggttg ctaattccat
    15901 tcttgctttt gtcgaacagg gcgatcatgt gttgatgacc aacaccgcct atgaaccgag
    15961 tcaggatttc tgtagcaaaa tcctcagcaa actgggcgta acgacatcgt ggtttgatcc
    16021 gctgattggt gccgatatcg ttaagcatct gcagccaaac actaaaatcg tgtttctgga
    16081 atcgccaggc tccatcacca tggaagtcca cgacgttccg gcgattgttg ccgccgtacg
    16141 cagtgtggtg ccggatgcca tcattatgat cgacaacacc tgggcagccg gtgtgctgtt
    16201 taaggcgctg gattttggca tcgatgtttc tattcaagcc gccaccaaat atctggttgg
    16261 gcattcagat gcgatgattg gcactgccgt gtgcaatgcc cgttgctggg agcagctacg
    16321 ggaaaacgcc tatctgatgg gccagatggt cgatgccgat accgcctata taaccagccg
    16381 tggcctgcgc acattaggtg tgcgtttgcg tcaacatcat gaaagcagtc tgaaagtggc
    16441 tgaatggctg gcagaacatc cgcaagtagc gcgagttaac caccctgctc tgcctggcag
    16501 taaaggacac gaattctgga aacgagactt tacaggtagc agcgggctat tttcctttgt
    16561 gcttaagaaa aaactcagta atgaagagct ggcgaactat ctggataact tcagtttatt
    16621 cagcatggcc tactcgtggg gcgggtatga atcgttgatc ctggcgaatc aaccagaaca
    16681 tatcgccgcc attcgcccac aaggcgagat cgattttagc gggaccttga ttcgcctgca
    16741 tattggtctg gaagatgtcg acgatctgat tgccgatctg gacgccggtt ttgcgcgaat
    16801 tgtataacat tgccactttt ggacaatttt gcagacattt aattgtgaaa agtcttaaat
    16861 tgttgcgtcc gggatcaagg cgtcccggac gaatcaggag tacaataggc agataaaggc
    16921 ttaaacgctg ttccacagga aagtccatgg ctgttattca agatatcatc gctgcgctct
    16981 ggcaacacga ctttgccgcg ctggcggatc ctcatattgt tagcgttgtt tactttgtca
    17041 tgtttgccac gctgttttta gaaaacggcc tgctgcccgc ctcatttttg ccaggcgaca
    17101 gcttgttgat actggcaggc gcattgattg cccagggggt tatggatttt ctgcctacga
    17161 ttgcgattct gaccgccgca gcaagtctgg gctgctggct aagttatatt caggggcgct
    17221 ggttagggaa taccaaaacg gtgaaaggct ggctggcaca gcttcctgct aaatatcacc
    17281 agcgcgccac ctgcatgttt gaccgccacg gtctgctggc gctgctggct ggacgttttc
    17341 ttgcatttgt ccgtacgctg ctgccaacca tggcgggaat ttccggtctg ccaaaccgcc
    17401 gcttccagtt tttcaactgg ttaagcggat tgctgtgggt cagcgtggta accagtttcg
    17461 gctatgcctt aagtatgatt ccgttcgtta aacgccatga agatcaggta atgacgttcc
    17521 tgatgatcct gccaattgcc ttgttaaccg ctggcttgtt aggcacgctg tttgtggtga
    17581 ttaaaaaaaa atactgtaac gcctgacgat tttccccgtt cccggttgct gtaccgggaa
    17641 cgtatttaat tcccctgcat cgcccgcatt cttgccgcat cttcccccgg cgtcacaccg
    17701 aagtaacgtt taaactcacg gctaaattgc gatgcgcttt catagccgac gcgcatcgcc
    17761 gccgcgctgg ccttcatgcc gtcgtggatg atcatcatcc gcgccttatg cagacggtaa
    17821 ttcttcaaat actgcaacgg cgaggtgctg gtgacagact taaaattatg gtggaacgcc
    17881 gatacgctca tgttggcttc tgccgccagt tgctcgacgc tcaggttttc ggtgtattta
    17941 ttctcaatcc gtttcagcac gcggctaatc aggctgaagt gagtctggcg actgaccagt
    18001 gccagtaacg cgccgccgca aggtccggtc agcacgtagt acagaatttc gcggataatc
    18061 tgtttgccga gaatgcgcgc atccagcggt cgctccatca catccagtaa ccgctctgcc
    18121 gcgcataaaa tctcttctga taacgtggcg gagttaatac cgctggctgc cattgacggc
    18181 tgaaaatgtt catcttcgcc aatgtccatc aacagttcct gtaactgcaa aatatcgaca
    18241 ttgagacgca accctgccag cggcacctct gacgtggcat aggtttcgca ctcaaacggc
    18301 aacggcaccg tcagcaggag atattcatta gcgtcataac gaaacacgcg ttcattgata
    18361 taaccgattt tatgtccgga aaagagaatt atgatgccag gctcgtacat caccggtgtg
    18421 cgtgcgaaag gcgtctcgcc atacaacaaa cgcacatcgg gcaacagctc tgacaaacta
    18481 ttttctttat ttttcagttt attaacttta tccgccagca agcggcaaat ctcttcacgt
    18541 ttcatatcgc gtaatttctt aggaataatg cggcaatttg attgtgcgca attttgtagc
    18601 atttctccag cactctggag aaataggcaa gacattggca gaaatgagca ttgagaggta
    18661 cggcgctggc gaccacaatg aaaaacatca ggcagaccga tctctgccct catattgacc
    18721 cagcaaaggg agcaagtaat gaacaacttt aatctgcaca ccccaacccg cattctgttt
    18781 ggtaaaggcg caatcgctgg tttacgcgaa caaattcctc acgatgctcg cgtattgatt
    18841 acctacggcg gcggcagcgt gaaaaaaacc ggcgttctcg atcaagttct ggatgccctg
    18901 aaaggcatgg acgtgctgga atttggcggt attgaaccaa acccggctta tgaaacgctg
    18961 atgaacgccg tgaaactggt tcgcgaacag aaagtgactt ttctgctggc ggttggcggc
    19021 ggttctgtac tggacggcac caaatttatc gctgcagcgg ctaactatcc ggaaaatatc
    19081 gatccgtggc acattctgca aacgggcggc aaagagatta aaagcgccat cccgatgggc
    19141 tgtgtgctga cgctgccagc aaccggttca gaatccaacg caggcgcggt gatctcccgt
    19201 aaaaccacag gcgacaagca ggcgttccat tctgcccatg ttcagcccgt atttgccgtg
    19261 ctcgatccgg tttataccta caccctgccg tcgcgtcagg tggctaacgg cgtagtggac
    19321 gcctttgtac acaccgtgga acagtatgtt accaaaccgg ttgatgccaa aattcaggac
    19381 cgtttcgcag aaggcatttt gctgacgctg atcgaagatg gtccgaaagc cctgaaagag
    19441 ccagaaaact acgatgtgcg cgccaacgtc atgtgggcgg cgactcaggc gctgaacggt
    19501 ttgatcggcg ctggcgtacc gcaggactgg gcaacgcata tgctgggcca cgaactgact
    19561 gcgatgcacg gtctggatca cgcgcaaaca ctggctatcg tcctgcctgc actgtggaat
    19621 gaaaaacgcg ataccaagcg cgctaagctg ctgcaatatg ctgaacgcgt ctggaacatc
    19681 actgaaggtt cagacgatga gcgtattgac gccgcgattg ccgcaacccg caatttcttt
    19741 gagcaattag gcgtgccgac ccacctctcc gactacggtc tggacggcag ctccatcccg
    19801 gctttgctga aaaaactgga agagcacggc atgacccaac tgggcgaaaa tcatgacatt
    19861 acgctggatg tcagccgccg tatatacgaa gccgcccgct aagcttttta cgcctcaaac
    19921 tttcgttttc gggcatttcg tccagactta agttcacaac acctcaccgg agcctgctcc
    19981 ggtgagttca tataaaggag gaacgtatgg ctaatccaac cgttattaag ctacaggatg
    20041 gcaatgtcat gccccagctg ggactgggcg tctggcaagc aagtaatgag gaagtaatca
    20101 ccgccattca aaaagcgtta gaagtgggtt atcgctcgat tgataccgcc gcggcctaca
    20161 agaacgaaga aggtgtcggc aaagccctga aaaatgcctc agtcaacaga gaagaactgt
    20221 tcatcaccac taagctgtgg aacgacgacc acaagcaccc ccgcgaagcc ctgctcgaca
    20281 gcctgaaaaa actccagctt gattatatcg acctctattt aatgcactgg cccgttcccg
    20341 ctatcgacca ttatgtcgaa gcatggaaag gcatgatcga attgcaaaaa gagggattaa
    20401 tcaaaagcat cggcgtgtgc aacttccaga tccatcacct gcaacgcctg attgatgaaa
    20461 ctggcgtgac gcctgtgata aaccagatcg aacttcaccc gctgatgcaa caacgccagc
    20521 tacatgcctg gaacgcgaca cacaaaatcc agaccgaatc ctggagccca ttagcgcaag
    20581 gagggaaagg cgttttcgat cagaaagtca ttcgcgatct ggcagataaa tacggcaaaa
    20641 cccctgcgca gattgttatc cgctggcatc tggatagcgg cctggtggtg atcccaaaat
    20701 cggtcacacc ttcacgcatt gtcgaaaact ttgatgtctg ggatttccgt ctcgacaaag
    20761 acgaacttgg cgaaattgca aaactcgatc agggcaagcg tctcggtccc gatcctgacc
    20821 agttcggcgg ctaacgtgca aattctcccg gtggcggtaa tgttccgcta ccggactttt
    20881 cagaaatcat ttattcccct cgcgtcccgc ccgttgctac tcttccttgt tcaggaatgc
    20941 caaatataag gacatcatca tgcagagccg gaagctctta aaagaacaac tcatctatat
    21001 ccgggataaa cgcaacggag aggtgaaaaa cagatgaaaa taatacttct gtttttatca
    21061 gccctggcaa gttttaccgt acacgcacag cccccctcac agaccgtaga acaaacagtc
    21121 cggcatattt atcagaacta taaatcagat gccactgccc cttattttgg tgaaaccgga
    21181 gagcgggcga taacatctgc gcgtattcaa caggcgctta ccctgaacga caatcttacg
    21241 ctgccgggca atattggctg gctggattat gatccggttt gtgattgtca ggattttggc
    21301 gatctggtgt tagaaagcgt agcgataacc caaactgacg ccgatcatgc cgatgccatt
    21361 gtgcgcttcc gtatctttca agacgataaa gaaaagacct cgcagacact gaaaatggtg
    21421 gcggaaaatg gtcgttgggt cattgacgat attgtcagca atcatggcag cgtcttacaa
    21481 gcggttaata gcgagaatga aaaaacactg gccgctatag cttcgttgca aaaagaacag
    21541 ccggaagcct ttgttgccga actctttgaa catattgctg attatagctg gccgtggacg
    21601 tgggtggttt ccgactctta ccgccaggcg gtcaatgcct tctataaaac caccttcaag
    21661 acggccaaca atcccgatga agatatgcca atagaacggc aatttattta cgacaatccg
    21721 atctgttttg gcgaagagtc gttattttca cgcgttgatg aaattcgagt cctggagaaa
    21781 accaccgatt ccgcccgcat tcatgttcgc tttacgctga ccaatggcaa caacgaagag
    21841 caagaactga ttttacagcg gcgcgaaggt aagtgggaaa tcgctgattt tatccgcccg
    21901 aacagcggca gcctacttaa gcagattgag gcaaaaaccg ccgccagatt aaaacaatga
    21961 gctgaattaa ataacaatta gccggaacaa taaataaaag ggaacactat atgaaaacga
    22021 ttttcaccgt gggagctgtt gttctggcaa cctgcttgct cagtggctgc gtcaatgagc
    22081 aaaagatcaa tcagctggcg agcaatgtgc aaacattaaa tgccaaaatc gcccggcttg
    22141 agcaggatat gaaagcatta cgcccacaaa tctatgctgc caaatccgaa gctaacagag
    22201 ccaatacgcg tcttgatgca caggactatt tcgactgcct gcgctgcttg cgtatgtacg
    22261 cagaatgata aaaaaatccc cggcagcatt ctgttaccgg ggatattttt taacgtccaa
    22321 ccgccgcttt agggcgtttc ttcgcaccag cattctgcag gcgagattgc gtagacgacg
    22381 ctttttttgc cgtagcaggc gtctgacgct gggtcgccat cggcgtatgt ttagtcaacg
    22441 ccggacgggt attgcggttc tgacggcgag cttcacgcat ctcttcaatg gttggcgcag
    22501 gcactaagca atcgcgacgg ctgccaatca gatgcttttt gcccatcgct tccagcgcct
    22561 ggcggattaa cggccagttt gccggatcgt ggtaacgcaa caacgcttta tgcaggcgac
    22621 gctgcttgtc gcccttcggt acgaagacgt cttcactctt ataaccaatc ttcgccagcg
    22681 ggtttttgcc ggtgtaatac atggtggtcg agttagccag cggcgacgga tagaagttct
    22741 gcacctggtc gaggcggaag cgatgctttt tcagccatag cgccagattc accatatctt
    22801 catcacgcgt acccgggtgc gcggagatga aatacgggat cagatactgt tctttacctg
    22861 cctgtttcga gtaagtatcg aacagctctt taaagcggtc atagctgccc atgcccggct
    22921 tcatcatctt cgataacggc ccttcttcgg tatgttccgg ggcaatcttc agataaccgc
    22981 caacgtgatg ggtcgccagc tctttgatat agcgcggatc ttctacggcg atgtcataac
    23041 gcacaccaga ggcaatcagg atctttttaa tgcctttcag atcgcgcgca cggcgataga
    23101 ggttaatcgt cggttcgtgg ttagtgtcca tatgcggaca aatatccgga taaacgcacg
    23161 acaaacgacg gcaggtttgc tcagcgcgtg gcgatttgca acgcaacata tacatgttgg
    23221 cagtaggccc accgagatcg gaaatcacgc ccgtaaaacc tggaacagtg tcgcggatcg
    23281 cttcgatctc attaataatc gaatcttcgg aacggctctg aataatgcgc ccttcgtgct
    23341 cggtaataga acagaaagag cagccgccaa agcagccacg cataatgttg accgagaaac
    23401 gaatcatttc gtaagccgga attcgggcat taccgtaagc cggatgcggc acgcgcttgt
    23461 acggcagcgc aaagacgcta tccatctctt cggtagaaag cggaatagca ggcgggttga
    23521 tccatacata gcggtcgcca tgtttttgca tcaatgcgcg ggcgcagcct gggttagttt
    23581 cgtggtgcag aatgcgcgaa gcatgggcgt acagcacttt atcgcccttc actttctcga
    23641 aagaaggcag caacacgtag gttttttccc acggtttcgg gcgtggtggc tgcacggtta
    23701 cagctttggc ttcctgcttt ttcggagcta ccggtttgtt atccgcgcac ggcaaatctt
    23761 cgccatacgg atgcgggatt gggtcgattt tccctggggt atcaagacgg gtggaatcca
    23821 caccgctcca gccaggcaac gcctctttta cgataatcgc ggtattacgc acatcgcgga
    23881 tttcactaat cggctcgccc atcgccagac ggtgcgccac ttccaccagc ggacgctcgc
    23941 cgttaccgaa catcagcatg tcggctttcg aatccaccag cacagaacgg cgcacggtat
    24001 cggaccagta atcataatgc gcggtacggc gcaggctggc ctcaataccg ccgaggatca
    24061 ccggcacatc tttccacgcc tctttacaac gttgggtata aaccagtgtg gcgcgatccg
    24121 gacgcttacc cgcgacgtta ttcggcgtgt aggcatcgtc atgacgtaaa cggcgatcgg
    24181 cggtatagcg gttgatcatc gagtccatgt tgccagcagt aacaccgaaa aacagattcg
    24241 gtttacccag acgcataaag tcgtctttac tgctccagtc tggctgggcg atgatcccga
    24301 cgcgaaagcc ctgcgcttcc agcatacgac cgcaaatcgc catcccgaag cttgggtgat
    24361 cgacatacgc gtcgccagta accaaaatga tgtcgcagct atcccagcca agttgatcca
    24421 tctcttcacg tgacatcggc aaaaacggtg ccggtccaaa acaggcggcc cagtactgcg
    24481 gccaggagaa caggtcgcga tccggttgga tcagggagat agagctcatt ttgcttccag
    24541 aaatggtaaa aaaataatca aaggccgggg attatacgct gtatcacgcg cggaaatgaa
    24601 gtgatgttga tgaacggtgg cgaaataccc tggtgaggtt tttcactgcc cgacttgggc
    24661 aagcacattt aaggctggcg gtatcgtggc aactgccgct actatttatg ccgatcagca
    24721 acgttcaaat catattgcac gacgatcggc tatccgagga cacccacagt aaggcaaggt
    24781 atggaacagg ttattacaaa gcgccgctat tacgatattg gtctacagat tgaagagctg
    24841 ctgtattcag gcgtatttaa ggccggagaa cgcttacctt cagaacgtga actgagcgag
    24901 cggtttaata ccagccgaac taccattcgt gaagccatca ttatgctgga actgaaaggc
    24961 gtattgaatg tgaagcaagg gtcagggatt ttctttgtcg actccaccga taagctcaac
    25021 cagaaatcat taatgcccta ttcagaaatt ggcccgtttg aactgttaca ggcacgtcag
    25081 gtaattgaga gcaatattac cggttttgct gccagccaga tcagctttaa cgagttacaa
    25141 gagttgaaga aaatcattgg attacaggaa aaagccatcg ctgcggaaag tgataaattt
    25201 gaagacctgg atcaccgatt tcatagcatt attgccgaag cgacgcaaaa tcgggtactc
    25261 atcaaacaag ctgctgaatt atggcgggca gttcgtacag aaaacccccg ctggaagaaa
    25321 cttaactaca agtacctgca tgaaaagcat ttgcgtctgc aatggctgga agatcaccgg
    25381 gctattttcc tggctctgca acagaaagat tcagagctcg ccagggaagc gtcctggaga
    25441 catctggaaa acagcaaaaa tgaactgatc aaaatcttca aacaagacgc ttccatcagc
    25501 gattttgatg actttttctt cgctcgttaa ccttcattag tccagagctg gcggaaaaaa
    25561 taacgccggc tctgccttcc gccctccact ggtacacgct ctctttttaa ttggtctaat
    25621 aaatcgacta tcatcagata tcttagatta gcggcaatta tcctaaaaac tgacctctaa
    25681 aaaactttcc tttcatttta ttcatataac tcattgatac agatataaat aacgccttgt
    25741 tgttctgttt cgtactttta cctttctctg caagcgtgat gcatctctct gtttttggtc
    25801 taatgaatta tgcaaattgg tatacacaca agccaccagg agtaagagca taatgtcagg
    25861 gaacgaaagt atcgatgccg cccactatga tcaggtaacc acctggcctc ggggcgggtt
    25921 acaggcagat attgtccata ttggttttgg cgcttttcat cgcggacacc aggctgtcta
    25981 cacggatctc actaatcaac tttcggacac ccgctggggg atctttgaga ttaacctgtt
    26041 tggtgatgct caactgatcg aaaacttaaa tgcgcaaaat gggctgtttt cggttgtgga
    26101 aacatctgca tcgcaatcta cctcacgcct tgtgcgttcg gtggctggcg gtattcatac
    26161 ccccagagat ggcattgccg cagcaatcca taaactggct gaacctcagg taaaaatcgt
    26221 ttcattaacc atcaccgaga aaggttattg cctcgatccg caaacacgtt cacttgatct
    26281 caccaatgga ttaatccaac acgatctaca aaacccggat gcgcctctgt cagctattgg
    26341 cgtgatcgtc tgtgctttgc aacaacgtaa agcgacagga ctcgccgctt ttagtgtgct
    26401 ctcctgtgac aacttgccag acaatgggca tctgacgcgc aatgccgttc tcggttttgc
    26461 ccgacaactg gaccagcccc tggctcagtg gatcgaagaa aatgtctcat ttccaggcac
    26521 gatggtcgat cgcattgttc cggcaatgac tgaatcgcaa ttcgccttac tggaaactaa
    26581 aaccggttat gccgatccct gcgggatcgt ctgcgaatca tttcgtcagt gggtgatcga
    26641 agataatttt gtgcgcggac gaccggaatg ggataaagcc agcgcgatgt ttgtcagcaa
    26701 tgttcagcct tatgaagaga tgaagttacg catgttaaat ggtagccatt catttctggc
    26761 ttataacggc tcactggctg gctatgagtt tatctggcaa tgcatggaag acgctaattt
    26821 tcgttccatt acccgccaac tgatgattaa tgaacaagcc cgaacactta atccagactt
    26881 aaatatcaac atccaggaat acgccgacct gttaattgaa cgctttagca accgtaatgt
    26941 tgcacaccgt accgggcaaa tcgctatgga cggttcgcaa aagcttcccc agcgagcgct
    27001 gacgccctgg ctgaaattgc atcagcaaaa acaaaacaat gctgttctgt cactgctcgt
    27061 tgctggttgg ttgcattatg tcattgatgc tgttgagaaa agccagtctg tcgctgatcc
    27121 aatgaatgac caacttcagg cgctaataaa ggaacaacaa gacgcatggc aacaggcgct
    27181 cgcattactg caccttagcg ccatatttgg tgatttaagc aaccatcagc catttataaa
    27241 tgaaataaaa atcgcctttg caaatataaa aaacaaaggc atcaaggcca ccatcagcca
    27301 attattatcg gatgagcaga aatgaaaaca ttaatttgtc agcagcctgg cgttatggaa
    27361 tatgtggaaa aggatattcc cacaccagca gataatgaag tgctgttaaa aatcaaagct
    27421 gtgggtattt gtggtactga tattcacgct tttgccggca gacagccttt ttttagctac
    27481 ccacgtgtat taggtcatga aatatgcgcc gaagcggttt cgcgaggcag ccagtgccaa
    27541 acagcacagc taggccagcg ctattccgtc atcccatgca ttccgtgtgg cgagtgcgca
    27601 gcctgtcggg aagagaaaac gaactgctgc gaacgtgttt cgctgtatgg cgtgcatcag
    27661 gatgggggtt ttagtgagta ccttgcggta cgtgaagaca accttgtgcc tctccctgac
    27721 gaggtcagcg acagtgccgg agcattggtt gaatgtttcg ccattggtgc acatgccgtt
    27781 cgtcgggcag agatcaaggc tgaacaaaac gtactggtga ttggcgctgg gccaatcggt
    27841 ctggctaccg cagccatcgc cagggctaaa ggggcacatg ttgttgttgc tgatattgac
    27901 tgtcaacgtc gccagcacgt tgtggatcat ctggcgatta atgccttcaa tcccacacag
    27961 gaagatttta ttgccgcgct tcgtgaggcc tttggaggcg aactggcttg catagtactg
    28021 gatgcgacgg gaaataaagc ttcaatgagt catgatgtaa atcttattcg tcatggcggc
    28081 aaaattgttt tcatcggttt gtacattggt gaacttgtta ttgacgatcc gaccttccat
    28141 aaaaaagaga caacgttact cagcagccgc aatgccacac gggaagattt tgcgttggtg
    28201 attaaactga tgcgcagcaa taaaattcac gaaaatttaa tgaaaaacca ggcgttcgat
    28261 ttctttagtg ttggcgaaga ttaccagcgt aacgttgtag aaaataaaaa tatggtcaaa
    28321 ggtgtgatca ctttttaagg gaacaaatat gacaactgtt tatgtcagtg aagaaaattt
    28381 aaaatcactg gtgcaccaca aattacatac cgccgggctg gataccgaca ccacacaaca
    28441 ggtgacagat gttttagtac atgccgatat taccggtgtg cattcacatg gtgttatgcg
    28501 tgtcgaacat tattgcaccc gccttgctgc cggagggtta aataaagccc cgcagtttag
    28561 cattgagcaa atttcaccgt cagtggccat tctcgactct gacgacggaa tggggcattc
    28621 cgcattaatt agcgccactg agcacgctat taaactggcc cagcaagagg gtctcggttt
    28681 tgttagcgtt aaaaacacgt ctcactgtgg tgcgttatct tattttgcag agatgatcac
    28741 caacaaaggg ttggttgcta tcgtaatgac gcaaaccgat acctgcgtgg ctccccatgg
    28801 cggcgccgag cgctttttgg gaactaaccc catcgccttt ggtttcccgg tggaaaacag
    28861 ccatccgatg attgttgata tggcgaccag tgccacggct ttcggcaaaa tacttcatgc
    28921 aaaggaaacc ggaaaacata ttggtgaagg gctggcgata gataaaaacg gttatggcac
    28981 cactgatccg tataagattg aaaacctgct acctttcggc caacacaaag gttcaggcat
    29041 tgcactggct attgatgcac tgactggcgt gctgatgaat gcgaattttg gcaaccatat
    29101 tgttcgcatg tatggtgatt atgacaagat gcgtaagctg gcaagtttgg ttattgccat
    29161 cgatccgaaa aagctcggca atcctgtttt tgcaaaaact atggcgaaaa tggtcacgga
    29221 gctgcatgcc gttaaaccgg ctcctggtgt cgaaaaagtg ttagcgccga acgatccgca
    29281 aatgcactac aaagaaaaat gccaacagga aggtattccg gttccggcag gaatattcca
    29341 ttatctggca gagaattaat cactattttt tattataacg atagaaaagc gctgtattta
    29401 tcgtttctgt tatattaacc aggagcagga tatgaaagca cttcgtcctc tgacggcatc
    29461 gcttatgctg ttaaccagtt gtttattaat atctaatacc acactggcaa aaacaacatt
    29521 aaaactgagt cataatcagg ataaaagcca tgctgtccat aaagcattaa gctatttagc
    29581 ggataaaacc aaagagtatt ctaatggtga attagttatt cgcatttacc caaatgcgac
    29641 attaggtaat gagcgtgaat cactggaatt aatgaactcc ggtgcattac agatggttaa
    29701 ggttaatgcc gcatcactag aatcatttgc tccggattac agccttttca gtctgccgtt
    29761 cttattccgc gaccgtgatc actattatcg tgttctgcaa agtgatttag gtaaaaaaat
    29821 acttaattca tcagaaagca aaggttttgt tggtattacg tactatgacg gtggtgcgag
    29881 aagtttttat tccaataaac cgattacaaa acctgaagat ttagcgggaa tgaaaatcag
    29941 ggttcaacaa agccccagcg ccattgcaat gatgaaagca ctcggtggtg tcgctacccc
    30001 gatggcgcaa ggcgaactct acaccgcact tcagcaagga gtggttgatg gcggagagaa
    30061 caataccgtt gtctatgccg atatgcgtca cgccgaagtc gcaaaagtct attcacgtga
    30121 tgaacacacc atggtacccg atgtgttaat tatcagcacc gacgtgttga ataaacttgg
    30181 tgataaagag cgcacggcat tattaaaagc agccgatgag tcaatgatgc agatgaagga
    30241 cgtcatttgg cctgcggctg aaaaagaagc ctacgacaaa atgaaaggga tgaacgcaac
    30301 agttgttgat gttgataaat ccgctttcaa agaacgcgtc aaaccgttat acgatgaatt
    30361 caaggcgaaa gatgcacaat cagccaaaaa ccttgagcta gtagaaagta tgtaacccta
    30421 atgggcaggt aattcctgcc ttttcatgga agacttatgc gaacactcac tcatattctt
    30481 aataagatcc ttgccggatg ttgctgcatt attctcgcca ttatggtgtt ctgcgtaacc
    30541 tggcaagtta ttgcacgttt tatttttaac gctcccagca ctgtgcttga tgagttcacc
    30601 cagattttat ttatgtggat gattttgctt ggcggtgtat atactgcggg tttaaaaaaa
    30661 catctggcca ttgatttgtt ggctcaaaaa cttccggctg catctgttct gacgcttgac
    30721 agttttatcc aaattattat tacggtcttt gcggttattt tcatgatcta tggcgggaat
    30781 attgttgttg aaaaagcaca acatgttgga caaatctctc cagtattaaa atggccaatg
    30841 gataaagtat attgggttat gcctgtcagt gggcttattc tggtctggta ttcagtaatg
    30901 aatattattg acaactatcg taaacggaat tttcattaac gagggaaata tggactttga
    30961 atatatctac cccgtcttaa ttttatttgg cagttttgcc gtcatgctgg caataggtgt
    31021 gccaattact tttgcgattg gtctttcttc gctgttatct attattactg ccttaccacc
    31081 cgatgccgcc atttctgtga tttcgcaaaa gatgactgtg gggctggatg gctttacgct
    31141 attagccatt cccttcttcg tgttagccgg aaacattatg aataccggtg gtatagccag
    31201 acgactggtt aacctggcgc aagcattagt tgggcgtctt cctggctcac tggctcattg
    31261 taatatcctc gcgaatacac tgtttggtgc gatttcaggt tcagccgttg cgtcagccgc
    31321 agcggtaggt gggattatgt caccactgca agaaaaagag ggatatgatc cggcgttttc
    31381 agcagcggtt aatattgcct ctgcccccat tggcctgatg atcccaccga gcaatgtgtt
    31441 gattgtttat tccctcgcca gtggcgggac ttctgtcgct gcgttgttct tagctggata
    31501 tctgccgggc atcctcaccg ctgttgcctt aatgtttgta gctgcacttt atgcccgacg
    31561 tcaccattat ccggtggccg agcgcattaa ttaccgtcag tttttacagg tattccgcga
    31621 atcaattcct agcctgatgc ttatttttat cattattggc ggtattatcg cgggggtatt
    31681 tacgcctaca gaagcatcgg caattgcggt aatttatagt ttagccctgg cgatgattta
    31741 ccgggaaatc actcttaaga agctcaatga tattctgtta gactccgtag taaccagttc
    31801 aattgttctg ttactggtag gctgctcgat ggggatgtca tgggccatga cgaatgctga
    31861 tgttcctgag ttgatcaacg aactgattac ccgcgtttcg gataacaaat gggtcattct
    31921 ttttattatc aatatcattc tgttgatcgt cggtaccttt atggatatca cgccggcgat
    31981 cctgatattt acacctattt tcctgccgat cgcccagcat ctggggattg atccgattca
    32041 cttcggcatc attatggttt tcaacttaac cattggcctt tgcacaccac ctgtcggcac
    32101 cattctgttt gtggggtgca gcattggtaa ggtcagtatc gacagggcaa taaaaccatt
    32161 actgccgatg tttctggcat tgtttgtagt aatggcaatt atttgttatt tcccgcagct
    32221 tagtctgatg ctgccaggat tattttcgac ctgatacaca atgaagactg catactgagt
    32281 aattttcaat gccccaccac tctgtggggc attgaattgg tccattacgg taccggattg
    32341 accaacagtt gcccaatcga gccacggtcc gccatttcca gcgtctgact gttgaagtag
    32401 aacgggaagt gcgcccagga aggctgaccg aaataaacaa gcagctccac ttgtccgtca
    32461 acccaaacgg tatctttcca gcctctgtct tccgggaacg gcatcgcacc attcacgtta
    32521 cggatctgga acatcacgcc ttcaatatgg aacgcttgcg gctcgtccgc gcgcaccgtc
    32581 cagcgttccc acgttccttg ctgcgcggtg acatcaatac ggttgacgtc ccacagttgt
    32641 ccattaatac ccgggtcatc acccagacta atatcgcgac tgcgaatcgg cgaaccggcc
    32701 atgatttcag ttggcagcaa gcgcatcgga agactgtcag tgaccagcgg cagaaggccg
    32761 gttgggcgca gcgttagcac cagcgtagaa accagaatgc tggatggctc aaagaagcca
    32821 cgaatacgat caacaatgct cgccgcttcg ccacaggtaa tcgacacttc atcaccgttg
    32881 ctcatatcca ccagaatctc acgacgctcg cccggtgcca gcgaaagttg cttcaccgat
    32941 acaggagcgg gcaggaatcc ctgatcgcca gaaatcacat gtaacgggcg accatcgctc
    33001 atctgtagtt gatagcgacg agagttcgac gcgttcaaca gacgcaagcg cacccagcca
    33061 cgcgagactt caacgtacgg gctttgtaca ccgttaacca gcagcgtatc accaacaaag
    33121 ccgccgcttc ccggttcgtt atattctggc gtaccaaagt tatccagccg tttatcctga
    33181 ataatgaccg gaaaatcatc cacaccataa tggttgggga taggcagcga tttgctgact
    33241 tcatcttcca ccagccacat tccggcaagg ccgttataga cctgctgagc cgtgcggttg
    33301 ggagtattgg cgtgatacca cagagtagct gcgttctggc gaatgggcag tacgggtgcc
    33361 cagtcagcgt ttggcgacat catccgtgcc ggaccgccca tcagcggacc aggtacctgt
    33421 agcccggcca ccgtcattga gacattttct gtcaggcgat tgctgtaaat aagcttaaca
    33481 tcgtcgccct tccagacgcg gatagtcggc cccaggtaac gaccattgat tccccaaacc
    33541 gacgcgcgtg tccctagcgt aaatgaccag tgcgcacgtt gtacagtcat aaacagcggt
    33601 tgcccacggc gagattcaag cagcggcgga acgggtagcg gttgctgttg cccggctgcg
    33661 ctggccttca ggggaacagc gcctgcacaa agtgcaatcc ccgatgcctg aatgaactga
    33721 cgccgactga gtgacatatt tgctccatgt aaaactggct aaagtaatac cgtgcaggaa
    33781 agtgttcccc gcaaaaatac agattgccga cttaaacttt tccggcggct tcgcgttctg
    33841 cgacttcttt atcgagctcg gcgatttttt gttccattat cgaacgacaa tgggcagcca
    33901 actcgcgaac ctgatctttg ccatactgac tgacgtcaat tggcggcagc atttcgacaa
    33961 tcaccagacc gttgtgtagc cgattaagat taatcttatt cgaagttgta gagacgcaca
    34021 cgggtataat cgggacgccc gccgcaattg ccgcgtgaaa tgccccggtc ttgaacggca
    34081 gcaggccgcg accacggctg cgggttcctt ccgggaacat ccagatggaa atacggcgtt
    34141 ttttgaagtg attcactact tccgcaatag tgccgtgagc tttagtgcga ttgtttctgt
    34201 cgatcaataa gttgccggtt aaccagtaca actgcccgaa gaaggggatc cacagcaagc
    34261 tctttttacc taccgtcact gtcggcggct gcacaatgtt cgatgccgtc accatgtcat
    34321 aattgttctg gtggttagca atatagatag cattgccgta gctttcagcg tctgcaggtt
    34381 tgcggcactc cactttcagg ccgaaaagcg gcgcaagacg gccaaacata tgcccaaagg
    34441 tggccacatg tttcgggtta cgcgggctga aaaggcagta aatggagccg aatacacaga
    34501 ctaagatgct gtaaatcacg gtaataataa gacgaaagat atatagcata acaccctctg
    34561 aaggtctgag agactggcat tgtacgtcac ctgaaaatca ggttagggca ttgttaaaag
    34621 ccgctctctg gaaaagcgta ttgttaatcg caacgcatga ataaacaacg gtttcaaggg
    34681 aatttttatc gtttccggag attgaatacg ctgccggatg acgacttaac gtttcatccg
    34741 gcgttccttg caagcgggag gaaacagcgc cctccccggc atattactct tcgctatcac
    34801 cgctgctggc acggcgagga gagtcgatct caacacgatc gatacgctgc aaaccgcgca
    34861 tcaacgtacc gcggcgtcca cgttcgccag cgactttctg tagctcttcc ggacgcagtt
    34921 taattttgcg tttcccaaca tgaatggtca gcgtgctttg cggcggcaga acgtacagtt
    34981 gcgccaggcc atcctcgcca cgcgcggctt ctgccgatgg aatgttgata atcttgttgc
    35041 ctttgccctt cgacagctgc ggcagatcgc ttaccgggaa catcaacata cggcctgcct
    35101 gagtgattgc cagcagcata tcggaagcat cttcaatcac caccggcggc ataacatggg
    35161 cattttccgg taaggtgatc aaagccttac ctgcacggtt acgcgccacc agatcgttaa
    35221 aggtgcagac gaaaccgtaa cccgcatcgg aagccatcag cagtttctga tcgtcgcttt
    35281 ccatcagcat atggtcaacg gtcgctccag gcggcaacgt taatttgccg gtgagcggct
    35341 cgccctgacc acgcgccgac ggcagcgtaa tcgggtcgat ggcatagcta cgaccggtgg
    35401 aatcaacaaa cactaccggt tggttgctct tacctttcac cgccgctttg aagctatcgc
    35461 ccgctttata attcaggccc ggcgcgtcga tatcatggcc tttagcgctg cgtacccagc
    35521 ccatctgcga cagcacaatg gtgacaggtt cagacggcag catgtcgtgc tcgctcatcg
    35581 ctttcgcttc ttcgcgttcc tgcaacggcg aacgacgttc gtcaccgtag gcttgcgcgt
    35641 ctgcctgcag ttctttcttc agcaggttat tcattttacg ctcggaagcc aaaatgccct
    35701 gcaactggtc gcgctctttt tccagctcac tctgctcacc gcgaatcttc atctcttcca
    35761 gtttggcaag atgacgcagt ttcagttcga ggatcgcttc cgcctgggtt tccgtaaggc
    35821 caaaccgcga catcagcgcc ggtttcggtt catcttcatt acgaatgatc tcaatcactt
    35881 cgtcgatatt gagaaacgcc accagcaaac cttcgaggat atgcaggcgc ttgaggactt
    35941 tctccagacg atagttcagt cggcggcgca cggtatcgcg gcggaacacc agccattcgg
    36001 agaggatttc cagcaggttt ttcaccgccg gacgaccatc cagaccgatc atattgaggt
    36061 taatgcgata gctcttttcc agatcggtgg tagcaaagag gtggttcatc acctgatcca
    36121 tatccacgcg gttggaacgc ggcacaatca ccagacgggt cgggttctcg tggtcagatt
    36181 catcgcgcag atcgtcaacc atcggcagct ttttgttgcg catttgcgca gcaatttgct
    36241 ccagtacgcg cgcacctgaa acctgatgcg gcaatgcgct gataaccacc gcgccatctt
    36301 ctttcttcca caccgcgcgc atacgcactg aaccacgtcc gttctcgtag attttacgaa
    36361 tctcggcgcg cgaagtgata atttccgctt cagtcggata atccggcccc tgcacgatat
    36421 ccagcagctg atcgagagtg gttttcggct ggtcgattaa tgcgattgcc gcctgagcca
    36481 cttcacgcag gttatgcggt ggaatatcgg tcgccatgcc gacggcaata ccggtggtgc
    36541 cgttaagcaa aatgtttggc agacgggcag gcagcatttt cggctcctgc aaagtgccgt
    36601 cgaagtttgg cacccagtca gccgtcccct gccccagctc gctcaatagc agctcggaat
    36661 atttcgacaa ccgggattcg gtgtaacgca ttgccgcgaa cgatttcgga tcgtccggcg
    36721 caccccagtt cccctgacca tcaaccagcg gataacggta agagaacggc tgcgccatca
    36781 ggaccatcgc ttcataacag gcactatcgc cgtgcggatg gtatttaccc agtacgtcac
    36841 cgacggtacg ggccgatttt ttaaatttgg cgctggcatt caggcccagc tcagacatcg
    36901 catacacaat gcggcgctga acaggtttca gaccatcacc aataaacggc aacgcacggt
    36961 ccatgatcac gtacatggag tagtttaagt aggcgttttc cgtaaattca tgtagcgcaa
    37021 ggcgctctgc catatcgctc attaattctg attcctcaac ttattcgcca gccttcaaag
    37081 gggcaatatt gccggagata ctacattatc tgccgcgtcg agtcacagga gatcagcgtg
    37141 aatagcgccg ccggacgtgc tccgacggcg ataaaggcat caatgtgcct tgatatacaa
    37201 ctctttgctg tagacataat cctggggatt atgcagcgga aaaccgccaa cgtagggttt
    37261 aagcagtttg attaacggct gatagtagat gggaatcagc ggtgcctgct ggttgatgat
    37321 cacttctgcc tgctgataca acgcattacg ctttgtcgca tcagtgatct gcgtggcctg
    37381 gtttagtaag gcgtcatact gcgcattttt ccagtgaccg acgttttctt cactatcgct
    37441 tttgagcgtg ttcaggaagc tggaggcatc attgtacgtc gcatcccacg actgccgaga
    37501 cagcataaaa tcaccggctc gtcgggcatc aagataggtt ttccactcca ttgtgcgcag
    37561 cgtcacctgt gcacccagcc attttttcca ttcggaagac aacgctatcg cggtcttttc
    37621 atgcagatcg tacttgttgt agaacagctc aaagcgtagc ggatgagagg cgtcgtatcc
    37681 cgcctgtttc agcaagactt ttgccatcgc gacgcgctca ctcattggct tttgcagttc
    37741 atcgaacgtc gtcgcgctaa agccttttac ctctggcggc gtcagcgtgg ttgcgggcgt
    37801 tctcaacccc agtacctttt gcgcaataag ctgtcgatca accgtaagat atagcgcccg
    37861 acgcacccgc acatcgttaa atggcggttt ctcaaggtta aagttgtaat attcgctgtt
    37921 cagacgcggg ataattcgta gctcgccagg cagtgatttt tcaatggcgg gaatttgctg
    37981 cgccggaacc caggtgagat cgacctctcc cgcacgatag cggttatagc cggtgaccga
    38041 attatccagc gccagatact caacctgttg caatactgta tgttgcgcat cgcggtactt
    38101 tggatttttg cgtgcagtaa tcttttcgtt aactacccat ttatcaagca caaaggcacc
    38161 gttgtaaacc atgttctctg gcttactcca gctatcgcca tgtttagcga tgacatgatg
    38221 aggaaccggg aacagcgtcg gccaggcgag catcgtcgtg aaccaaggaa ctggctgctc
    38281 aagcgtaact tcaagagtac gatcatccgt cgctttgaca cccagcgatg taacatctgc
    38341 tttacccgca acaatagccg cggcattgtt aatgtgcgcc tgtgccagat agccagcaaa
    38401 agggcttgcc gttttcgggt caaccgcgcg ctgccagccg aggacaaaat cctctgccgt
    38461 cagaggctga ccgtctgacc actgcaaacc gctacgcaga tggaaaatat agcgcttgcc
    38521 gtcgtcaagt atctcccagc gttcagcctg agcgggctgc acctggcctt caccgtccat
    38581 ccataccaga ccttcaaaca gatccagcac aatctgcgcg gcagtattct cctctacctt
    38641 ttgcgggtcg agcgtgcctg ggtcgctatg attgttgtaa cgaaagactt gttgcggggc
    38701 gagcggtgtg ttggcgggaa cgtcagcagc gtagagagga gccgcactta ccaggctgac
    38761 cagccataat aaatttcgcg tatacatggt attccttacc ggagaaatta taagcccgac
    38821 aacaatacgc ctgtggcatt gtttgctcaa acttatcgcg agtgatttgg ctcacactcc
    38881 ggtaaagatt ccccatcacg ccaacggcac ataaatatcc gtttccagtc gccgctctgt
    38941 catcccttcg gcaagattgg tgtagtgaaa caggatcggg gctttgcgca ttttttcgcc
    39001 gcttgcaggt agccagtggc gaatgatgcc ccatatcgta tgtgaaatat cgtccagctc
    39061 gccaacatgg cgggcaacag cataacgccc accggtaagt tcgccattac tcacgccata
    39121 acgattatcg gggatcggtt cgctaacgct gccgcagata tcaaagcgaa acgcttccgg
    39181 tgcggtggtg gcagggtcgt cccaggcgac gccaaaagtc tggctttggt taacaggaga
    39241 aagccccgtc tctttacgcc acatgataaa tttcgccacg ctgtagttga gcaactcagg
    39301 gctacagcgg tgcggcaaca tcgccacagg gattgatggg aaatcgataa tatttacatc
    39361 cagtgtcagg tttgtcattt tgcctccctg cgaaattatt cccgggaacg cgtcccggga
    39421 gcggtaacaa ttacggattt actttgcgga tctgtttgac gtcaatttca acagagttcc
    39481 agtctttatc gacttccccc tgaatctcaa ccgtatcttt cggcgtcacc gtcacgccgt
    39541 tccagcgttt gtggtcgata tcaacattga tagtaccgct ggcatctttg aacacgtaga
    39601 gatcgtcaga gatgcgttca acgatattgc cgcgtagggt tacccaggtg tcgtcacgca
    39661 gggattttgc gctttctacg gtcgttacgc tgccgttcgg cccctggaat cctccggcct
    39721 gactttgcgt tgccgatggg ccagaaaaac ctccctgctc tgctgccata accggtgcac
    39781 tgcacagggc cattactgcg attactgctg cgaatttttt catgtttatt actcccttta
    39841 atgtctgttt ccgagcattt aacaagatag tccttaacaa cttcttaagg gaaaaaaata
    39901 aaatttaatg ctgtacagag cgcgttacaa cacggtttac tggcagcaaa tacggttatc
    39961 gcagggatga aaaaatgcga attttactga tagaagatga catgctgatt ggcgacggca
    40021 tcaaaacggg ccttagtaaa atgggtttta gcgtcgactg gtttacacaa ggtcgtcagg
    40081 gaaaagaagc gctatatagc gcgccttatg atgcggtgat cctggattta accttaccag
    40141 gcatggatgg tcgcgatatt ttgcgcgaat ggcgaggaaa aggtcagcgt gagcctgtat
    40201 tgatcctgac tgcgcgcgat gcgctggcgg aacgtgtaga agggctgcgt ctgggagctg
    40261 acgattatct gtgtaaacct tttgcgttga tagaagtcgc cgccaggctg gaagctctga
    40321 tgcgccgaac caacggccag gccagcaacg agctgcgcca cggcaacgtc atgctcgacc
    40381 ccggcaaacg tatcgccacg ctggctggcg aacccttaac gctgaaacca aaagaatttg
    40441 ccctgctgga attactgatg cgtaacgctg gtcgggtact gccgcgcaaa ctgattgaag
    40501 agaaactgta tacctgggac gaagaggtca ccagtaatgc cgttgaagtg catgtgcatc
    40561 atctgcgacg caaactcggt agtgatttta ttcgtaccgt tcatggtatt ggctacacat
    40621 taggtgagaa atgaaattta cccaacgtct tagtctgcga ggcaggctga cgctaatctt
    40681 tttaattctg gcctcggtga cctggctgct ttccagcttt gtcgcctgga aacaaacaac
    40741 ggataacgtc gatgagttgt tcgacaccca actgatgctg tttgccaaac ggttaagtac
    40801 gctcgatctc aacgaaatca acgcggcgga tcgcatggca cagacgccaa ataaattaaa
    40861 acacggtcat gttgatgacg atgcgctgac ctttgccatc tttacccacg acggcagaat
    40921 ggtccttaac gatggcgata acggagaaga tattccctat agctatcagc gggaaggttt
    40981 tgctgacggg caactggtcg gtgaagacga tccctggcgt tttgtctgga tgacctcacc
    41041 tgatggcaaa tatcgcatcg ttgtgggtca ggaatgggaa taccgtgaag acatggcgct
    41101 ggcgattgtt gccggacaat tgatcccgtg gctggtcgca ctgccgatta tgttaatcat
    41161 catgatggta ctactgggtc gtgaactcgc gccgctgaac aaactggcgc tggcactacg
    41221 tatgcgtgac ccagactcgg aaaaaccact aaacgcgacg ggcgtaccca gcgaagtgcg
    41281 cccactggtt gagtcgctaa atcaactgtt cgcccgcaca catacgatga tggttcgtga
    41341 acgacgcttt acctccgacg cagctcacga acttcgtagc ccgttaacgg cgctgaaagt
    41401 gcaaaccgaa gttgctcagc tctctgacga tgatccgcag gcgcggaaaa aagcgctgct
    41461 ccaattacat tccgggatcg atcgcgctac tcgtctggtt gatcaactgc tcacgctatc
    41521 gcggctggac tcactggata accttcagga cgtcgcggag atcccgcttg aagatctcct
    41581 gcaatcgtcg gtgatggata tttaccacac ggcgcagcag gcgaaaattg acgtgcgact
    41641 gacactcaaa gccaacggca tcaaacgcac cggacaaccg ctattgctaa gtttgttggt
    41701 gcgaaatttg ctggataacg ccgtgcgcta cagtccacaa ggtagcgtgg tagacgtcac
    41761 gctgaatgcc gataatttca tcgtgaggga taacggcccc ggtgtgacgc cagaggcact
    41821 ggcgcgaatt ggcgaacgct tctatcgccc acccggacaa accgctaccg gcagtggact
    41881 tgggctatcg attgtccagc gaatcgccaa gctgcatgac atgaatgttg aatttgggaa
    41941 tgcgaaacaa ggtggatttg aagcgaaggt aagctggtaa cttctctgtc tatgagagcc
    42001 gttaaaacga ctctcataga ctttactaat agcaaaatat aaaccgtccc caaaaaagcc
    42061 accaaccaca acccagacaa ataacgagcg agaatacata taaccaaaac tcgaacattt
    42121 cccgcataaa gagttttctt aagataagaa taataagtgg tgtcagaaga aaaaatgctg
    42181 caatcattgc ttgcaatata cgatcttcgc ctgaaacaga aggtatactg caaatattgt
    42241 ctcccgactc aagcatccag tcatacttat tgaaagcaaa aaaagaatc
//
LOCUS       DAHHTG010000025        42177 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.025, whole genome shotgun sequence.
ACCESSION   DAHHTG010000025 DAHHTG010000000
VERSION     DAHHTG010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 42177)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42177)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42177
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.025"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            <1..354
                     /gene="rplT"
                     /locus_tag="NBI74_001364"
     CDS             <1..354
                     /gene="rplT"
                     /locus_tag="NBI74_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HCD1931726.1"
                     /translation="ARVKRGVIARARHKKILKQAKGYYGARSRVYRVAFQAVIKAGQY
                     AYRDRRQRKRQFRQLWIARINAAARQNGISYSKFINGLKKASVEIDRKILADIAVFDK
                     VAFTALVEKAKAALA"
     misc_feature    471..595
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            477..521
                     /gene="pheM"
                     /locus_tag="NBI74_001365"
     CDS             477..521
                     /gene="pheM"
                     /locus_tag="NBI74_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pheST operon leader peptide PheM"
                     /protein_id="HCD1931727.1"
                     /translation="MNAAIFRFFFYFST"
     gene            805..1788
                     /gene="pheS"
                     /locus_tag="NBI74_001366"
     CDS             805..1788
                     /gene="pheS"
                     /locus_tag="NBI74_001366"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004851795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HCD1931728.1"
                     /translation="MSHLAELVASAKAAISQASDVAALDNVRVEYLGKKGHLTLQMTT
                     LRELPPEERPAAGAVINEAKEQVQQALNARKAELESAALNARLAAETIDVSLPGRRIE
                     NGGLHPVTRTIDRIESFFGELGFTVATGPEIEDDYHNFDALNIPGHHPARADHDTFWF
                     DATRLLRTQTSGVQIRTMKAQQPPIRIIAPGRVYRNDYDQTHTPMFHQMEGLIVDTNI
                     SFTNLKGTLHDFLRNFFEEDLQIRFRPSYFPFTEPSAEVDVMGKNGKWLEVLGCGMVH
                     PNVLRNVGIDPEVYSGFAFGMGMERLTMLRYGVTDLRSFFENDLRFLKQFK"
     gene            1803..4190
                     /gene="pheT"
                     /locus_tag="NBI74_001367"
     CDS             1803..4190
                     /gene="pheT"
                     /locus_tag="NBI74_001367"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HCD1931729.1"
                     /translation="MKFSELWLREWVNPAIDSDALANQITMAGLEVDGVEPVAGSFHG
                     VVVGEVVECAQHPNADKLRVTKVNVGGDRLLDIVCGAPNCRQGLRVAVATIGAVLPGD
                     FKIKAAKLRGEPSEGMLCSFSELGISDDHNGIIELPADAPIGTDIREYLKLDDNTIEI
                     SVTPNRADCLGIIGVARDVAVLNQLPLVEPEIVPVGATIDDTLPIAVEAPDACPRYLG
                     RVVKGINVKAPTPLWMKEKLRRCGIRSIDAVVDVTNYVLLELGQPMHAFDKDRIEGGI
                     VVRMAKEGETLVLLDGTEAKLNADTLVIADHNKALAMGGIFGGEHSGVNDETQNVLLE
                     CAFFSPLSITGRARRHGLHTDASHRYERGVDPALQHKAMERATRLLIDICGGEAGPVI
                     DITNEATLPKRATITLRRSKLDRLIGHHIADEQVTDILRRLGCEVTEGKDEWQAVAPS
                     WRFDMEIEEDLVEEVARVYGYNNIPDEPVQASLIMGIHREADLSLKRVKTLLNDKGYQ
                     EVITYSFVDPKVQQMIHPGVEALLLPSPISVEMSAMRLSLWTGLLATVVYNQNRQQNR
                     VRIFESGLRFVPDTQAPLGIRQDLMLAGVICGNRYEEHWNLAKETVDFYDLKGDLESV
                     LDLTGKLNEVEFRAEANPALHPGQSAAIYLKGERIGFVGVVHPELERKLDLNGRTLVF
                     ELEWNKLADRVVPQAREISRFPANRRDIAVVVAENVPAADILSECKKVGVNQVVGVNL
                     FDVYRGKGVAEGYKSLAISLILQDTSRTLEEEEIAATVAKCVEALKERFQASLRD"
     gene            4195..4494
                     /gene="ihfA"
                     /locus_tag="NBI74_001368"
     CDS             4195..4494
                     /gene="ihfA"
                     /locus_tag="NBI74_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HCD1931730.1"
                     /translation="MALTKAEMSEYLFDKLGLSKRDAKELVELFFEEIRRALENGEQV
                     KLSGFGNFDLRDKNQRPGRNPKTGEDIPITARRVVTFRPGQKLKSRVENASPKDE"
     gene            4595..5575
                     /gene="btuC"
                     /locus_tag="NBI74_001369"
     CDS             4595..5575
                     /gene="btuC"
                     /locus_tag="NBI74_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter permease BtuC"
                     /protein_id="HCD1931731.1"
                     /translation="MLTLARQQQRQNIRWLLCLSVLMLLALLLSLCAGEQWISPGDWF
                     TPRGELFVWQIRLPRTLAVLLVGAALAISGAVMQALFENPLAEPGLLGVSNGAGVGLI
                     AAVLLGQGQLPNWALGLCAIAGALIITLILLRFARRHLSTSRLLLAGVALGIICSALM
                     TWAIYFSTSVDLRQLMYWMMGGFGGVDWRQSWLMLALIPVLLWICCQSRPVNMLALGE
                     ISARQLGLPLWFWRNVLVAATGWMVGVSVALAGAIGFIGLVIPHILRLCGLTDHRVLL
                     PGCALAGASALLLADIVARLALAAAELPIGVVTATLGAPVFIWLLLKAGR"
     gene            5638..6189
                     /gene="btuE"
                     /locus_tag="NBI74_001370"
     CDS             5638..6189
                     /gene="btuE"
                     /locus_tag="NBI74_001370"
                     /EC_number="1.11.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional thioredoxin/glutathione peroxidase"
                     /protein_id="HCD1931732.1"
                     /translation="MQDSILTTVVKDIDGEVTTLEKYAGNVLLIVNVASKCGLTPQYE
                     QLENIQKAWADRGFVVLGFPCNQFLEQEPGSDEEIKTYCTTTWGVTFPMFSKIEVNGE
                     GRHPLYQKLIAAAPTAVAPEESGFYARMVSKGRAPLYPDDILWNFEKFLVGRDGLVIQ
                     RFSPDMTPEDPIVMESIKLALAK"
     gene            6189..6938
                     /gene="btuD"
                     /locus_tag="NBI74_001371"
     CDS             6189..6938
                     /gene="btuD"
                     /locus_tag="NBI74_001371"
                     /EC_number="7.6.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter ATP-binding protein
                     BtuD"
                     /protein_id="HCD1931733.1"
                     /translation="MSIVMQLQDVAESTRLGPLSGEVRAGEILHLVGPNGAGKSTLLA
                     RMAGMTSGKGSIQFAGQPLEAWSATKLALHRAYLSQQQTPPFAMPVWHYLTLHQHDKT
                     RTELLNDVAGALALDDKLGRSTNQLSGGEWQRVRLAAVVLQITPQANPAGQLLLLDEP
                     MNSLDVAQQNALDKILSALCQQGLAIVMSSHDLNHTLRHAHRAWLLKGGKMLASGRRE
                     EVLTPANLAQAYGMNFRRLDIEGHRMLISTI"
     gene            7016..7480
                     /locus_tag="NBI74_001372"
     CDS             7016..7480
                     /locus_tag="NBI74_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HCD1931734.1"
                     /translation="MRFCLILITALFLAGCSHHKAPPPNARLSDSITVIAGLNDQLQS
                     WHGTPYRYGGMTRRGVDCSGFVVVTMRDRFDLQLPRETKQQASIGTQIDKDELLPGDL
                     VFFKTGSGQNGLHVGIYDTNNQFIHASTSKGVMRSSLDNVYWQKNFWQARRI"
     gene            7728..8441
                     /gene="ydiV"
                     /locus_tag="NBI74_001373"
     CDS             7728..8441
                     /gene="ydiV"
                     /locus_tag="NBI74_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-FlhDC factor"
                     /protein_id="HCD1931735.1"
                     /translation="MKIFLENLYHSDCYFLPIRDNQQDLVGVELITHFSSEDGTVRIP
                     TSRVIAQLTEEQHWQLFSEQLELLKSCQHFFIQHKLFAWLNLTPQVATLLLDRDNFAG
                     ELLKYPFIELLINENYPHFNEGKDNRDLLSLSQMYPLVLGNLGAGNSTMKAVFDGLFT
                     RVMLDKSFIQQQITHRSFEPFIRAIQAQISPCCNCIIAGGIDTPEILAQIIPFDFHAL
                     QGCLWPAVPINQITTLVQR"
     gene            8504..9940
                     /locus_tag="NBI74_001374"
     CDS             8504..9940
                     /locus_tag="NBI74_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiU family protein"
                     /protein_id="HCD1931736.1"
                     /translation="MTLSFITRWRDELPETYTALSPTPLNNARLIWHNTELANTLSIP
                     SSLFKNGAGVWGGENLLPGMSPLAQVYSGHQFGVWAGQLGDGRGILLGEQLLADGTTM
                     DWHLKGAGLTPYSRMGDGRAVLRSTIRESLASEAMHYLGIPTTRALSIVTSDSPVYRE
                     TVEPGAMLMRVAPSHLRFGHFEHFYYRREPEKVRQLADFAIRHYWPHLADDEDKYRLW
                     FTDVVARTASLIAQWQTVGFAHGVMNTDNMSLLGLTLDYGPFGFLDDYEPGFICNHSD
                     HQGRYSFDNQPAVALWNLQRLAQTLSPFVAVDGLNEALDSYQQVLLTHYGQRMRQKLG
                     FMTELKEDNALLNELFSLMARERSDYTRTFRMLSLTEQHSAASPLRDEFIDRAAFDDW
                     FARYRGRLQQDEITDNERQQLMQSVNPALVLRNWLAQRAIEAAEKGDMTELHRLHEAL
                     RNPFSDRDDDYVSRPPDWGKRLEVSCSS"
     gene            complement(9944..10135)
                     /gene="hemP"
                     /locus_tag="NBI74_001375"
     CDS             complement(9944..10135)
                     /gene="hemP"
                     /locus_tag="NBI74_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemin uptake protein HemP"
                     /protein_id="HCD1931737.1"
                     /translation="MRYTDSRKLTPETDANHKTASPQPIRRITSQTLLGPDGKLIIDH
                     DGQEYLLRKTQAGKLLLTK"
     gene            complement(10267..11313)
                     /gene="aroH"
                     /locus_tag="NBI74_001376"
     CDS             complement(10267..11313)
                     /gene="aroH"
                     /locus_tag="NBI74_001376"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroH"
                     /protein_id="HCD1931738.1"
                     /translation="MNRTDELRTARIESLVTPAELALRYPVTPGVATHVTDSRRRIEK
                     ILNGEDKRLLVIIGPCSIHDLTAAMEYATRLQSLRNQYQSRLEIVMRTYFEKPRTVVG
                     WKGLISDPDLNGSYRVNHGLELARKLLLQVNELGVPTATEFLDMVTGQFIADLISWGA
                     IGARTTESQIHREMASALSCPVGFKNGTDGNTRIAVDAIRAARASHMFLSPDKNGQMT
                     IYQTSGNPYGHIIMRGGKKPNYHADDIAAACDTLHEFDLPEHLVVDFSHGNCQKQHRR
                     QLEVCEDICQQIRNGSTAIAGIMAESFLREGTQKIVGGQPLTYGQSITDPCLGWEDTE
                     RLVEKLASAVDTRF"
     gene            complement(11470..12303)
                     /gene="ppsR"
                     /locus_tag="NBI74_001377"
     CDS             complement(11470..12303)
                     /gene="ppsR"
                     /locus_tag="NBI74_001377"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="posphoenolpyruvate synthetase regulatory
                     kinase/phosphorylase PpsR"
                     /protein_id="HCD1931739.1"
                     /translation="MDNAVDRHVFYISDGTAITAEVLGHAVMSQFPVTISSITLPFVE
                     NESRARAVKDQIDAIYHQTGVRPLVFYSIVLPEIRAIILQSEGFCQDIVQALVAPLQQ
                     EMKLDPTPIAHRTHGLNPNNLNKYDARIAAIDYTLAHDDGISLRNLDQAQVILLGVSR
                     CGKTPTSLYLAMQFGIRAANYPFIADDMDNLVLPASLKPLQHKLFGLTIDPERLAAIR
                     EERRENSRYASLRQCRMEVAEVEALYRKNQIPWINSTNYSVEEIATKILDIMGLSRRM
                     Y"
     gene            12636..15014
                     /gene="ppsA"
                     /locus_tag="NBI74_001378"
     CDS             12636..15014
                     /gene="ppsA"
                     /locus_tag="NBI74_001378"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HCD1931740.1"
                     /translation="MSNNGSSPLVLWYNQLGMNDVDRVGGKNASLGEMITNLSGMGVS
                     VPNGFATTADAFNQFLDQSGVNQRIYELLDKTDIDDVTQLAKAGAQIRQWIIDTPFQP
                     ELENAIREAYAQLSADDENASFAVRSSATAEDMPDASFAGQQETFLNVQGVDAVLVAV
                     KHVFASLFNDRAISYRVHQGYDHRGVALSAGVQRMVRSDLASSGVMFSIDTESGFDQV
                     VFITSAWGLGEMVVQGAVNPDEFYVHKPTLAANRPAIVRRTMGSKKIRMVYAPTQEHG
                     KQVKIEDVPQEQRDIFSLTNEEVQELAKQAVQIEKHYGRPMDIEWAKDGHTGKLFIVQ
                     ARPETVRSRGQVMERYTLHSQGKIIAEGRAIGHRIGAGPVKVIHDISEMNRIEPGDVL
                     VTDMTDPDWEPIMKKASAIVTNRGGRTCHAAIIARELGIPAVVGCGDATERMKDGENV
                     TVSCAEGDTGYVYAELLEFSVKSSSVETMPDLPLKVMMNVGNPDRAFDFACLPNEGVG
                     LARLEFIINRMIGVHPRALLEFDDQEPQLQNEIREMMKGFDSPREFYVGRLTEGIATL
                     GAAFYPKRVIVRLSDFKSNEYANLVGGERYEPDEENPMLGFRGAGRYVSDSFRDCFAL
                     ECEAVKRVRNDMGLTNVEIMIPFVRTVDQAKAVVEELARQGLKRGENGLKIIMMCEIP
                     SNALLAEQFLEYFDGFSIGSNDMTQLALGLDRDSGVVSELFDERNDAVKALLSMAIRA
                     AKKQGKYVGICGQGPSDHEDFAAWLMEEGIDSLSLNPDTVVQTWLSLAELKK"
     gene            complement(15071..16717)
                     /gene="fadK"
                     /locus_tag="NBI74_001379"
     CDS             complement(15071..16717)
                     /gene="fadK"
                     /locus_tag="NBI74_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="medium-chain fatty-acid--CoA ligase"
                     /protein_id="HCD1931741.1"
                     /translation="MKVTLTFNEQRRAAYRQQGLWGDASLADYWQQTARAMPDKIAVV
                     DNHGATYTYSALDHAASCLANWMLAKGIESGDRIAFQLPGWCEFTVIYLACLKTGAVS
                     VPLLPSWREAELVWVLNKCQAKMFFAPTLFKQTRPVDLILPLQNQLPQLQQIVGVDKL
                     APATSSLSLSQILADNTPLTTAITTHGDELAAVLFTSGTEGLPKGVMLTHNNILASER
                     AYCARLNLTWQDVFMMPAPLGHATGFLHGVTAPFLIGARSVLLDIFTPDACLALLEQQ
                     RCTCMLGATPFVYDLLNLLEKQPADLSALRFFLCGGTTIPKKVARECQQRGIKLLSVY
                     GSTESSPHAVVNLDDPLPRFMHTDGYAAAGVEIKVVDDARKTLPPGCEGEEASRGPNV
                     FMGYFDEPELTARALDEEGWYYSGDLCRMDEAGYIKITGRKKDIIVRGGENISSREVE
                     DILLQHPKIHDACVVAMPDERLGERSCAYVVLKAPHHSLSLEEVVAFFSRKRVAKYKY
                     PEHIVVIEKLPRTVSGKIQKFLLRKDIMRRLTQDVCEEIE"
     gene            complement(16774..17067)
                     /locus_tag="NBI74_001380"
     CDS             complement(16774..17067)
                     /locus_tag="NBI74_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HCD1931742.1"
                     /translation="MSQNATVNVDIKLGVDKFHVDEGHPHIILAANPDINEFRKLMKA
                     CPAGLYKQDDAGNIHFDSAGCLECGTCRVLCGNTILEQWQYPAGTFGIEFRYG"
     gene            complement(17064..18353)
                     /locus_tag="NBI74_001381"
     CDS             complement(17064..18353)
                     /locus_tag="NBI74_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HCD1931743.1"
                     /translation="MSDDKFDAIVVGAGVAGSVAALVMARAGLDVLVIERGDSAGCKN
                     MTGGRLYAHTLEAIIPGFAASAPVERKVTREKISFLTEESAVTLDFHREQPDVPQHAS
                     YTVLRNRLDPWLMEQAEQAGAQFIPGVRVDALVREGNKVTGVQAGDDILEANVVILAD
                     GVNSMLGRSLGMVSASNPHHYAVGVKEVIGLTPEQINDRFNVTGEEGAAWLFAGSPSD
                     GLMGGGFLYTNKDSVSLGLVCGLGDIAHAQKSVPQMLEDFKQHPAIRPLISGGKLLEY
                     SAHMVPEGGLAMVPQLVNDGVMIVGDAAGFCLNLGFTVRGMDLAIASAQAAATTVIAA
                     KERADFSASSLAQYKRELEQSCVMRDMQHFRKIPALMENPRLFSQYPRMVADIMNDMF
                     TIDGKPNQPVRKMIMGHAKKIGLINLLKDGIKGATAL"
     gene            complement(18408..19346)
                     /locus_tag="NBI74_001382"
     CDS             complement(18408..19346)
                     /locus_tag="NBI74_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit alpha"
                     /protein_id="HCD1931744.1"
                     /translation="MSQLNSVWVFSDNPERYAELFGSAQQWGQQVYAIVQNTEQAQAV
                     MPYGPKCIYVLEQNDALQRTENYAESIAALLKDKHPAMLLLAATKRGKALAARLSVQL
                     NAALVNDATAVDIVDGHICAEHRMYGGLAFAQEKINSPLAIITLAPGVQEPCTRDISH
                     QCPTETVPYVAPRHEILCRERRAKAASSVDLSKAKRVVGVGRGLAAQDDLKMVHELAA
                     VLNAEVGCSRPIAEGENWMERERYIGVSGVLLKSDLYLTLGISGQIQHMVGGNGAKVI
                     VAINKDKNAPIFNYADYGLVGDIYKVVPALISQLNR"
     gene            complement(19366..20130)
                     /locus_tag="NBI74_001383"
     CDS             complement(19366..20130)
                     /locus_tag="NBI74_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein"
                     /protein_id="HCD1931745.1"
                     /translation="MKIITCFKLVPEEQDIVVTPEYTLNFDNADAKISQFDLNAIEAA
                     SQLATDDDEIAALTVGGSLLQNSKVRKDVLSRGPHSLYMVQDAQLEHALPLDTAKALA
                     TAVEKIGFDLLIFGEGSGDLYAQQVGLLVGEILQLPVINAVSAIQRQGNTLVIERTLE
                     DDVEVIELSVPAVLCVTSDINVPRIPSMKAILGAGKKPVNQWQASDIGWSQSAPLAEL
                     TGIRVPPQTERKHIILDNDSPEAIAELAEHLKKALN"
     gene            20446..21357
                     /locus_tag="NBI74_001384"
     CDS             20446..21357
                     /locus_tag="NBI74_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HCD1931746.1"
                     /translation="MYQRCFDNASETLFVAGKTPRLSRFAFSDDPKWESGHHVHDNET
                     ELIYVKKGVARFTIDSSLYVAHADDIVVIERGRLHAVASDVNDPATTCTCALYGFQFQ
                     GAEENQLLQPHSCPVIAAGQGKEVIKTLFNELSVILPQSKNSQTSSLWDAFAYTLAIL
                     YYENFKNAYRSEQGYIKKDVLIKDILFYLNNNYREKITLEQLSKKFRASVSYICHEFT
                     KEYRISPINYVIQRRMTEAKWSLTNTELSQAEISWRVGYENVDHFAKLFLRHVGCSPS
                     DYRRQFKNCFAEQEILSEFPQPVSLAG"
     gene            complement(21400..22551)
                     /locus_tag="NBI74_001385"
     CDS             complement(21400..22551)
                     /locus_tag="NBI74_001385"
                     /EC_number="1.3.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HCD1931747.1"
                     /translation="MDFSLTEEQELLLASIRELITTNFPEEYFRTCDQNGTYPREFMR
                     ALADNGISMLGVPEEFGGIPADYVTQMLALMEVSKCGAPAFLITNGQCIHSMRRFGSA
                     EQLRKTAESTLETGDPAYALALTEPGAGSDNNSATTTYTRKNGKVYINGQKTFITGAK
                     EYPYMLVLARDPQPKDPKKAFTLWWVDSSKPGIKINPLHKIGWHMLSTCEVYLDNVEV
                     EESDMVGEEGMGFLNVMYNFEMERLINAARSTGFAECAFEDAARYANQRIAFGKPIGH
                     NQMIQEKLALMAIKIDNMRNMVLKVAWQADQHQSLRTSAALAKLYCARTAMEVIDDAI
                     QIMGGLGYTDEARVSRFWRDVRCERIGGGTDEIMIYVAGRQILKDYQNK"
     gene            complement(22565..24160)
                     /locus_tag="NBI74_001386"
     CDS             complement(22565..24160)
                     /locus_tag="NBI74_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl CoA:acetate/3-ketoacid CoA transferase"
                     /protein_id="HCD1931748.1"
                     /translation="MKPVKPPRINGRVPVLSAQEAVNYIPDEATLCVLGAGGGILEAT
                     TLITALADKYKQTQTPRNLSIISPTGLGDRADRGISPLAQEGLVKWALCGHWGQSPRI
                     SDLAEQNKIIAYNYPQGVLTQTLRAAAAHQPGIISDIGIGTFVDPRQQGGKLNEVTKE
                     DLIKLVEFDNKEYLYYKAIAPDIAFIRATTCDSEGYATFEDEVMYLDALVIAQAVHNN
                     GGIVMMQVQKMVKKATLHPKSVRIPGYLVDIVVVDPDQTQLYGGAPVNRFISGDFTLD
                     DSTKLSLPLNQRKLVARRALFEMRKGAVGNVGVGIADGIGLVAREEGCADDFILTVET
                     GPIGGITSQGIAFGANVNTRAILDMTSQFDFYHGGGLDVCYLSFAEVDQHGNVGVHKF
                     NGKIMGTGGFIDISATSKKIVFCGTLTAGSLKTEITDGKLNIVQEGRVKKFIRELPEI
                     TFSGKIALERGLDVRYITERAVFTLKEDGLHLIEIAPGVDLQKDILDKMDFTPVISPE
                     LKLMDERLFIDAAMGFVLPEAAH"
     gene            complement(24307..25065)
                     /gene="aroD"
                     /locus_tag="NBI74_001387"
     CDS             complement(24307..25065)
                     /gene="aroD"
                     /locus_tag="NBI74_001387"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HCD1931749.1"
                     /translation="MKTVTVKDLVIGTGAPKIIVSLMAKDIASVKSEALAYREADFDI
                     LEWRVDHYADLSNVESVMAAAKILRETMPEKPLLFTFRSAKEGGEQAISTEAYIALNR
                     AAIDSGLVDMIDLELFTGDDQVKETVAYAHAHDVKVVMSNHDFHKTPEAEEIIARLRK
                     MQSFDADIPKIALMPQSTSDVLTLLAATLEMQEQYADRPIITMSMAKTGVISRLAGEV
                     FGSAATFGAVKKASAPGQISVNDLRTVLTILHQA"
     gene            complement(25096..25962)
                     /gene="ydiB"
                     /locus_tag="NBI74_001388"
     CDS             complement(25096..25962)
                     /gene="ydiB"
                     /locus_tag="NBI74_001388"
                     /EC_number="1.1.1.282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinate/shikimate dehydrogenase"
                     /protein_id="HCD1931750.1"
                     /translation="MDVTAKYELIGLMAYPIRHSLSPEMQNKALEKAGLPFTYMAFEV
                     DNDSFPGAIEGLKALKMRGTGVSMPNKQLACEYVDELTPAAKLVGAINTIVNDDGYLR
                     GYNTDGTGHIRAIKESGFDIKGKTMVLLGAGGASTAIGAQGAIEGLKEIKLFNRRDEF
                     FDKALAFAQRVNENTDCVVTVTDLADQQAFAEALASADILTNGTKVGMKPLENESLVN
                     DISLLHPGLLVTECVYNPHMTKLLQQAQQAGCKTIDGYGMLLWQGAEQFTLWTGKDFP
                     LEYVKQVMGFGA"
     gene            complement(25974..27239)
                     /locus_tag="NBI74_001389"
     CDS             complement(25974..27239)
                     /locus_tag="NBI74_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCD1931751.1"
                     /translation="MSQNKAFSTPFILAVLCIYFSYFLHGISVITLAQNMTSLAEKFS
                     TDNAGIAYLISGIGLGRLISILFFGVISDKFGRRAVILMAVIMYLLFFFGIPACPNLT
                     LAYCLAVCVGIANSALDTGGYPALMECFPKASGSAVILVKAMVSFGQMFYPMLVSYML
                     LNNIWYGYGLIIPGILFVLITLMLLKSKFPSQLVDASVANELPQMNSKPLVWLEGVSS
                     VLFGVAAFSTFYVIVVWMPKYAMAFAGMSEAEALKTISYYSMGSLVCVFIFAALLKKM
                     VRPIWANVFNSALATITAAIIYLYPSPLVCNAGAFVIGFSAAGGILQLGVSVMSEFFP
                     KSKAKVTSIYMMMGGLANFVIPLITGYLSNIGLQYIIVLDFTFALLALITAIIVFIRY
                     YRVFIIPENDVRFGEHKFSTRLNTIKYRG"
     gene            complement(27466..28680)
                     /locus_tag="NBI74_001390"
     CDS             complement(27466..28680)
                     /locus_tag="NBI74_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCD1931752.1"
                     /translation="MKNPYYPTALGLYFNYLVHGMGVILMSLNMASLETLWQTNAAGV
                     SIVISSLGIGRLSVLLFAGLLSDRFGRRPFIMLGMCCYMAFFFGILHTNNIIIAYVFG
                     FLAGMANSFLDAGTYPSLMEAFPRSPGTANILIKAFVSSGQFLLPLIISLLVWAELWF
                     GWSFMIAAGIMFINALFLYRCTFPPHPGRHLPVIKKTTSSTEHRCSIIDLASYSLYGY
                     ISMATFYLVSQWLAQYGQFVAGMSYTMSIKLLSIYTVGSLLCVFITAPLIRNTVRPTT
                     LLMLYTFISFIALLTVCLHPTFYVVIIFAFVIGFTSAGGVVQIGLTLMAERFPYAKGK
                     ATGIYYSAGSIATFTIPLITAHLSQRSIADIMWFDTAIAAIGFILALFIGLRSRKETR
                     HHTLKENIAPGG"
     gene            complement(28780..29136)
                     /locus_tag="NBI74_001391"
     CDS             complement(28780..29136)
                     /locus_tag="NBI74_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707588.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiL family protein"
                     /protein_id="HCD1931753.1"
                     /translation="MNAYELQALRHIFAMTIDECATWIAQTGNSESWRQWENGKCAIP
                     DYVVEQLLAMRQQRKKHLHAIIEKINNRIGNNTMRFFPDLTAFQQVYPDGNFIDWKIY
                     QSVAAELYAHDLERLC"
     gene            complement(29272..29379)
                     /gene="rprA"
                     /locus_tag="NBI74_001392"
     ncRNA           complement(29272..29379)
                     /ncRNA_class="antisense_RNA"
                     /gene="rprA"
                     /locus_tag="NBI74_001392"
                     /product="antisense sRNA RprA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00034"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00034"
     gene            complement(29565..30677)
                     /gene="ydiK"
                     /locus_tag="NBI74_001393"
     CDS             complement(29565..30677)
                     /gene="ydiK"
                     /locus_tag="NBI74_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter YdiK"
                     /protein_id="HCD1931754.1"
                     /translation="MVNVRQPRDVAQILLSVLFLAIMIVACLWIVQPFILGFAWAGTV
                     VIATWPVLLRLQKIMFGRRSLAVLVMTLLLVMVFIIPIALLVNSIVDGSGPLIKAISS
                     GDMTLPDLAWLNTIPVIGAKLYAGWHNLLDMGGTAIMAKVRPYIGTTTTWFVGQAAHI
                     GRFMVHCALMLLFSALLYWRGEQVAQGIRHFATRLAGVRGDAAVLLAAQAIRAVALGV
                     VVTALVQAVLGGIGLAVSGVPYATLLTVLMILSCLVQLGPLPVLIPAIIWLYWTGDTT
                     WGTVLLVWSGVVGTLDNVIRPMLIRMGADLPLILILSGVIGGLIAFGMIGLFIGPVLL
                     AVSWRLFAAWVEEVPPPTDQPEEILEELGEIEKSNK"
     gene            30891..33947
                     /locus_tag="NBI74_001394"
     CDS             30891..33947
                     /locus_tag="NBI74_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HCD1931755.1"
                     /translation="MIPQISQAPGVVQLVLNFLQELEQQGFTGDTATSYADRLTMSTD
                     NSIYQLLPDAVVFPRSTADVALIARLAAQERYSSLIFTPRGGGTGTNGQALNQGIIVD
                     MSRHMNRIIEINPEEGWVRVEAGVIKDQLNQYLKPFGYFFAPELSTSNRATLGGMINT
                     DASGQGSLVYGKTSDHVLGVRAVLLGGDILDTQPLPVELAETLGKSNTTIGRIYNTVY
                     QRCRQQRQLIIDNFPKLNRFLTGYDLRHVFNDEMTEFDLTRILTGSEGTLAFITEARL
                     DITRLPKVRRLVNVKYDSFDSALRNAPFMVEARALSVETVDSKVLNLAREDIVWHSVS
                     ELITDVPDKEMLGLNIVEFAGDDEALIDERVNALCVRLDELIASQQAGVIGWQVCREL
                     AGVERIYAMRKKAVGLLGNAKGAAKPIPFAEDTCVPPEHLADYIAEFRALLDSHGLSY
                     GMFGHVDAGVLHVRPALDMCDPQQEILMKQISDDVVALTAKYGGLLWGEHGKGFRAEY
                     SPAFFGEELFAELRKVKEAFDPHNRLNPGKICPPEGLDAPMMKVDAVKRGTFDRQIPI
                     AVRQQWRGAMECNGNGLCFNFDARSPMCPSMKITQNRIHSPKGRATLVREWLRLLADR
                     GVDPLKLEQELPESGVSLRTLIARTRNSWHANKGEYDFSHEVKEAMSGCLACKACSTQ
                     CPIKIDVPEFRSRFLQLYHTRYLRPLRDHLVATVESYAPLMARAPKTFNFFINQPLVR
                     KLSEKHIGMVDLPLLSVPSLQQQMVGHRSANMTLEQLEALNAEQKARTVLVVQDPFTS
                     YYDAQVVADFVRLVEKLGFQPVLLPFSPNGKAQHIKGFLNRFAKTAKKTADFLNRMAK
                     LGMPMVGVDPALVLCYRDEYKLALGEERGEFNVLLANEWLASALESQPVATVSGESWY
                     FFGHCTEVTALPGAPAQWAAIFARFGAKLENVSVGCCGMAGTYGHEAKNHKNSLGIYE
                     LSWHQAMQRLPRNRCLATGYSCRSQVKRVEGTGVRHPVQALLEIIK"
     gene            33944..34354
                     /gene="menI"
                     /locus_tag="NBI74_001395"
     CDS             33944..34354
                     /gene="menI"
                     /locus_tag="NBI74_001395"
                     /EC_number="3.1.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA hydrolase"
                     /protein_id="HCD1931756.1"
                     /translation="MIWKRKITLEALNAMGEGNMVGLLDIRFEHIGDDTLEATMPVDS
                     RTKQPFGLLHGGASVVLAESIGSVASYLCTEGEQKVVGLEINANHVRSAREGRVRGVC
                     KPLHLGSRHQVWQIEIFDEKGRLCCSSRLTTAIL"
     gene            34454..34642
                     /locus_tag="NBI74_001396"
     CDS             34454..34642
                     /locus_tag="NBI74_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiH family protein"
                     /protein_id="HCD1931757.1"
                     /translation="MSTQLDPTQLAIEFLRRDQSNLSPAQYLKRLKQLELEFADLLTL
                     SSAELKEEIYFAWRLGVH"
     gene            35190..35558
                     /gene="sufA"
                     /locus_tag="NBI74_001397"
     CDS             35190..35558
                     /gene="sufA"
                     /locus_tag="NBI74_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold SufA"
                     /protein_id="HCD1931758.1"
                     /translation="MDMHSGTFNPQDFAWQGLTLTPAAAIHIRELVAKQPGMIGVRLG
                     VKQTGCAGFGYVLDSVSEPDKDDLLFEHDGAKLFVPLQAMPFIDGTEVDFVREGLNQI
                     FKFHNPKAQNECGCGESFGV"
     gene            35567..37054
                     /gene="sufB"
                     /locus_tag="NBI74_001398"
     CDS             35567..37054
                     /gene="sufB"
                     /locus_tag="NBI74_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416198.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HCD1931759.1"
                     /translation="MSRNTEATDDVKTWTGGPLNYKEGFFTQLATDELAKGINEEVVR
                     AISAKRNEPEWMLEFRLNAYHAWLEMEEPHWLKAHYDKLNYQDYSYYSAPSCGNCDDN
                     CASEPGAVQQTGANAFLSKEVEAAFEQLGVPVREGKEVAVDAIFDSVSVATTYREKLA
                     EQGIIFCSFGEAIHDHPELVRKYLGTVVPGNDNFFAALNAAVASDGTFIYVPKGVRCP
                     MELSTYFRINAEKTGQFERTILVADEDSYVSYIEGCSAPVRDSYQLHAAVVEVIIHKN
                     AEVKYSTVQNWFPGDNNTGGILNFVTKRALCEGENSKMSWTQSETGSAITWKYPSCIL
                     RGDNSIGEFYSVALTSGHQQADTGTKMIHIGKNTKSTIISKGISAGHSQNSYRGLVKI
                     MPTATNARNFTQCDSMLIGANCGAHTFPYVECRNNSAQLEHEATTSRIGEDQLFYCLQ
                     RGISEEDAISMIVNGFCKDVFSELPLEFAVEAQKLLAISLEHSVG"
     gene            37064..37810
                     /gene="sufC"
                     /locus_tag="NBI74_001399"
     CDS             37064..37810
                     /gene="sufC"
                     /locus_tag="NBI74_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HCD1931760.1"
                     /translation="MLSIKELHVSVEDKAILRGLSLDVRPGEVHAIMGPNGSGKSTLS
                     ATLAGREDYEVTSGTVEFKGKDLLALSPEDRAGEGIFMAFQYPVEIPGVSNQFFLQTA
                     LNAVRSYRGQETLDRFDFQDLMEEKIALLKMPEDLLTRSVNVGFSGGEKKRNDILQMA
                     VLEPELCILDESDSGLDIDALKVVADGVNSLRDGKRSFIIVTHYQRILDYIKPDYVHV
                     LYQGRIVKSGDFTLVKQLEEQGYGWLTEQQ"
     gene            37785..39056
                     /gene="sufD"
                     /locus_tag="NBI74_001400"
     CDS             37785..39056
                     /gene="sufD"
                     /locus_tag="NBI74_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HCD1931761.1"
                     /translation="MAGLPNSSNALQQWHHLFEAEGAKRSPQAQQHLQQLLRTGLPTR
                     KHENWKYTPLDGLTNSQFVSIAGEISPQQRDALALTLDAVRLVFVDGRYVPALSDATE
                     GSGYEVSINDDRQGLPDAIQAEVFLHLTESLAQSVTHIAVKRGQRPAKPLLLMHITQG
                     VAGEEVNTAHYRHHLDLAEGAEATVIEHFVSLNDARHFTGARFTINVAANAHLQHIKL
                     AFENPLSHHFAHNDLLLADDATAFSHSFLLGGAVLRHNTSTQLNGENSTVRINSLAMP
                     VKNEVCDTRTWLEHNKGFCNSRQLHKTIVSDKGRAVFNGLINVTQHAIKTDGQMTNNN
                     LLMGKLAEVDTKPQLEIYADDVKCSHGATVGRIDDEQMFYLRSRGINQQDAQQMIIYA
                     FAAELTEALRDEGLKQQVLARIGQRLPGGAR"
     gene            39053..40273
                     /gene="sufS"
                     /locus_tag="NBI74_001401"
     CDS             39053..40273
                     /gene="sufS"
                     /locus_tag="NBI74_001401"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase SufS"
                     /protein_id="HCD1931762.1"
                     /translation="MTFSVDKVRADFPVLSREVNGLPLAYLDSAASAQKPSQVIDAEA
                     EFYRHGYAAVHRGIHTLSAQATEKMENVRKRASLFINARSAEELVFVRGTTEGINLVA
                     NSWGNSNVRAGDNIIISQMEHHANIVPWQMLCARVGAELRVIPLNPDGTLQLETLPTL
                     FDEKTRLLAITHVSNVLGTENPLAEMITLAHQHGAKVLVDGAQAVMHHPVDVQALDCD
                     FYVFSGHKLYGPTGIGILYVKEALLQEMPPWEGGGSMIATVSLSEGTTWTKAPWRFEA
                     GTPNTGGIIGLGAALEYVSALGLNNIAEYEQNLMHYALSQLESVPDLTLYGPQNRLGV
                     IAFNLGKHHAYDVGSFLDNYGIAVRTGHHCAMPLMAYYNVLAMCRASLAMYNTHEEVD
                     RLVTGLQRIHRLLG"
     gene            40286..40702
                     /gene="sufE"
                     /locus_tag="NBI74_001402"
     CDS             40286..40702
                     /gene="sufE"
                     /locus_tag="NBI74_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfuration protein SufE"
                     /protein_id="HCD1931763.1"
                     /translation="MALLPDKEKLLRNFLRCANWEEKYLYIIELGQRLPELRDEDKSP
                     QNSIQGCQSQVWIVMRQNAQGIIELQGDSDAAIVKGLIAVVFILYDQMTPQDIVNFDV
                     RPWFEKMALTQHLTPSRSQGLEAMIRAIRAKAAALS"
     gene            40851..41855
                     /gene="ldtE"
                     /locus_tag="NBI74_001403"
     CDS             40851..41855
                     /gene="ldtE"
                     /locus_tag="NBI74_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtE"
                     /protein_id="HCD1931764.1"
                     /translation="MKRASLLTLTLIGAFSAIQAAWAVDYQLPPTGSRLVGQNQTYTV
                     QEGDKNLQAIARRFDTAAMLILEANNTIAPVPKPGTTITIPSQLLLPDAPRQGIIVNL
                     AELRLYYYPPGENIVQVYPIGIGLQGLETPVMETRVGQKIPNPTWTPTAGIRQRSLER
                     GIKLPPVVPAGPNNPLGRYALRLAHGNGEYLIHGTSAPDSVGLRVSSGCIRMNAPDIK
                     ALFSSVRTGAPVKVINEPVKYSVEPNGMRYVEVHRPLSAEEQQNVQTMPYTLPAGFTQ
                     FKDNKAVDQKLVDKALYRRAGYPVAVSSGATPAASNAPSVESAQNGEPEQGNMLRATQ
                     "
     gene            complement(41918..42154)
                     /gene="lpp"
                     /locus_tag="NBI74_001404"
     CDS             complement(41918..42154)
                     /gene="lpp"
                     /locus_tag="NBI74_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein lipoprotein Lpp"
                     /protein_id="HCD1931765.1"
                     /translation="MKATKLVLGAVILGSTLLAGCSSNAKIDQLSSDVQTLNAKVDQL
                     SNDVNAMRSDVQAAKDDAARANQRLDNMATKYRK"
BASE COUNT        10573 a        10304 c        10922 g        10378 t
ORIGIN      
        1 gctcgcgtaa aacgtggtgt tattgcacgc gcacgtcaca agaaaatttt gaaacaagct
       61 aaaggctact acggtgcgcg ttctcgcgta taccgcgttg ccttccaggc tgttatcaaa
      121 gctggtcagt atgcttaccg cgaccgtcgt caacgtaagc gtcagttccg tcaactgtgg
      181 attgcgcgta tcaacgcagc agcacgtcag aatggtattt cttacagcaa attcatcaat
      241 ggcctgaaaa aagcctctgt tgaaatcgac cgtaagatcc tggctgatat cgcagtattc
      301 gacaaagtag cgttcaccgc tctggttgaa aaagcgaaag cagctctggc ataagccagt
      361 tgaaagaggg agctagtctc cctcttttcg tttcaacgcc atcaaaacat tgacttttat
      421 cgccgtagcc ttttcaataa aggtcttttg aagagtacca aaaggtaacg caagcaatga
      481 atgctgctat tttccgcttc tttttttact ttagcacctg actccaggag gctagcgcgt
      541 gagaagagaa acggaaaaca gcgcctgaaa gcctcccagt ggaggctttt tttgtatgcg
      601 cgttttgaaa aataaaaagc ctgtctcatc aggcgtaatt gtcgcagcca gtctggacac
      661 ggccagcgcg gagaaaccgg accgtactta agtacgtgag gatttcgagc actgcccggg
      721 accaaaatgg caagtaaaat agcctgatgg gatgggctct aagtccaacg aaccagtgtc
      781 accactgaca caatgaggaa aaacatgtca catctcgcag aactggttgc cagtgcgaag
      841 gcggccatta gccaggcgtc agatgttgcc gcgttagata atgtgcgcgt cgaatatttg
      901 ggtaaaaaag ggcacttaac ccttcagatg acgaccctgc gtgagctgcc gccagaagag
      961 cgtccggcag ctggtgcggt tatcaatgaa gcgaaagagc aggttcagca ggcgctgaat
     1021 gcgcgtaaag cggaactgga aagcgcagca ctgaatgcgc gtctggcggc ggaaacgatt
     1081 gatgtctccc tgcctggtcg tcgcattgaa aacggcgggc tgcatccggt tacccgtacc
     1141 atcgaccgta tcgaaagttt cttcggtgag cttggcttta ccgtggcaac cgggccggaa
     1201 atcgaagacg attatcataa cttcgatgct ctgaacattc ctggtcacca ccctgcgcgc
     1261 gctgaccacg acactttctg gtttgacgct acccgcctgc tgcgtaccca gacctctggc
     1321 gtacagatcc gcaccatgaa agcccagcag ccaccgattc gtatcatcgc gcctggccgc
     1381 gtttatcgta acgactacga ccagactcac acgccgatgt tccatcagat ggaaggtctg
     1441 attgttgata ccaacatcag ctttactaac ctgaaaggca cgctgcacga cttcctgcgt
     1501 aacttctttg aggaagattt gcagatccgc ttccgtcctt cctacttccc gtttaccgaa
     1561 ccttctgcag aagtggacgt catgggtaaa aacggaaaat ggctggaagt actgggctgc
     1621 gggatggtgc atccgaacgt gctgcgtaac gttggcatcg acccggaagt ttactctggt
     1681 ttcgccttcg ggatggggat ggagcgtctg actatgttgc gctacggcgt caccgacctg
     1741 cgttcattct ttgaaaacga tctgcgtttc ctcaaacagt ttaaataagg caggaataga
     1801 ttatgaaatt cagtgaactg tggttacgcg aatgggtgaa cccggcgatt gatagcgacg
     1861 cgctggcaaa tcaaatcacg atggcgggcc tggaagttga tggtgtagaa ccggttgccg
     1921 gtagcttcca cggcgtggtt gtgggtgaag tggttgagtg tgcgcagcat ccgaacgctg
     1981 acaaactgcg tgtgacaaaa gtgaatgtcg gcggcgatcg cctgctggac atcgtctgcg
     2041 gtgcgccaaa ctgccgtcag ggcctgcgtg tggcggtagc gaccattggt gctgttctgc
     2101 cgggtgattt caaaattaaa gccgcgaaac tgcgtggcga accgtctgaa gggatgctgt
     2161 gctccttctc tgagctggga atttctgacg atcataacgg cattatcgag ctgcctgcgg
     2221 atgcgccgat tggcaccgac atccgcgaat acctgaaact cgatgacaac accatcgaaa
     2281 tcagcgtgac accaaaccgt gccgactgct taggtatcat tggtgttgcg cgcgacgttg
     2341 ccgtgttgaa ccagctgccg ctggttgaac cggaaatcgt tccggttggt gcgaccatcg
     2401 acgacacgct gccgattgca gtagaagcgc cggatgcctg cccgcgctat cttggccgtg
     2461 ttgtaaaagg cattaacgtt aaagcgccaa ctccgctgtg gatgaaagaa aaactgcgtc
     2521 gttgcgggat ccgttctatc gatgcagttg ttgacgtcac caactatgtg ctgctcgaac
     2581 tgggccagcc gatgcacgct ttcgataaag atcgcattga aggcggcatt gtggtgcgga
     2641 tggcaaaaga gggtgaaacg ctggtattgc tcgacggtac tgaagcgaag ctgaatgctg
     2701 acaccctggt catcgccgac cacaacaagg cgctggcgat gggcggcata tttggtggcg
     2761 aacactctgg cgtgaatgac gaaacgcaaa acgttctgct ggaatgcgct ttcttcagcc
     2821 cgctgtctat caccggtcgt gctcgtcgtc atggcctgca tactgatgcg tctcaccgtt
     2881 atgagcgtgg cgttgatccg gcactgcagc acaaagcgat ggaacgtgcg acccgtctgc
     2941 tgatcgacat ctgcggtggt gaagcgggtc cggtaattga tatcactaac gaagcaacgc
     3001 tgccgaagcg tgcaaccatc actttacgtc gtagcaaact ggatcgcctg atcggccatc
     3061 atattgctga tgagcaggta acagacattc tgcgtcgtct cggctgcgaa gtgaccgaag
     3121 gcaaagacga gtggcaggca gttgcgccga gctggcgttt cgacatggag attgaagaag
     3181 atctggtcga agaagtcgcg cgtgtttacg gctacaacaa catcccggat gagccggtac
     3241 aggcaagcct gattatgggg attcaccgtg aagctgacct gtcgctcaag cgcgtaaaaa
     3301 cgctgctcaa cgacaaaggc tatcaggaag tgatcaccta cagcttcgtt gatccgaaag
     3361 tgcagcagat gatccatcca ggcgttgaag ccttactgct gccaagcccg atctctgttg
     3421 aaatgtcagc aatgcgtctt tctctgtgga ccggcctgct ggcaaccgtg gtgtacaacc
     3481 agaaccgtca gcagaatcgt gtacgcattt tcgaaagcgg tctgcgtttc gtgccagata
     3541 ctcaggcacc gttgggtatt cgtcaggatc tgatgttagc cggtgtgatt tgcggtaacc
     3601 gttacgaaga gcactggaac ctggcaaaag agaccgttga tttctatgat ttgaaaggcg
     3661 atcttgaatc cgttctcgac ctgaccggta aactgaatga ggttgagttc cgtgcagaag
     3721 cgaatccggc actgcatccg gggcaatccg cagcgattta tctgaaaggt gaacgtattg
     3781 gttttgttgg ggttgttcat cctgaactgg aacgtaaact ggatcttaac ggtcgcactc
     3841 tggtgttcga actggagtgg aacaagctcg cagaccgcgt ggtgcctcag gcgcgcgaga
     3901 tttctcgctt cccggcgaac cgtcgtgaca tcgcggtggt ggtcgcagaa aacgttcccg
     3961 cagcggatat tttatccgaa tgtaagaaag ttggcgtaaa tcaggtagtt ggcgtaaact
     4021 tatttgacgt gtaccgcggt aagggtgttg cggaggggta taagagcctc gccataagcc
     4081 tgatcctgca agataccagc cgtacactcg aagaagagga gattgccgct accgtcgcca
     4141 aatgtgtaga ggcattaaaa gagcgattcc aggcatcatt gagggattga acctatggcg
     4201 cttacaaaag ctgaaatgtc agaatatctg tttgataagc ttgggcttag caagcgggat
     4261 gccaaagaac tggttgaact gtttttcgaa gagatccgtc gcgctctgga aaacggcgaa
     4321 caggtgaaac tctctggttt tggtaacttc gatctgcgtg ataagaatca acgcccggga
     4381 cgtaacccga aaacgggcga ggatattccc attacagcac ggcgcgtggt gaccttcaga
     4441 cccgggcaga agttaaaaag ccgggtcgaa aacgcttcgc ccaaagacga gtaatctgat
     4501 ctaactaaaa aggccgctct gcggcctttt ttcttttcac tgtcgaagag tcaccgtaaa
     4561 atcaacgcca tgacacttca gcagaacgga taccatgctg acacttgccc gccaacaaca
     4621 gcgacaaaat attcgctggt tattatgcct gtcagttttg atgctgctgg cgcttctctt
     4681 aagcctttgc gccggtgaac aatggatctc gccaggtgac tggtttactc ctcgtggcga
     4741 actgttcgtc tggcaaattc gcctgccacg tacgctggct gtattgctgg ttggtgctgc
     4801 gttggctata tccggcgctg taatgcaggc gttgtttgaa aatcctctgg cagaacctgg
     4861 actacttggc gtctctaacg gcgcaggcgt ggggcttatc gccgcggtat tgcttgggca
     4921 agggcaactc cccaactggg cgctggggct gtgtgcgatt gctggcgcgc ttatcatcac
     4981 tttaatactc ttacgtttcg cccgtcgtca tctttcgacc agtcggttat tgctggctgg
     5041 cgttgcatta gggattatct gtagtgcact aatgacgtgg gctatctact tttccacctc
     5101 tgttgattta cgtcagctga tgtactggat gatgggcggt tttggcggcg tagactggcg
     5161 gcaaagctgg ctgatgctgg cattgatccc cgtgttgttg tggatctgtt gtcagtccag
     5221 gccggtgaat atgttagcac ttggcgagat ctcggcgcgg caactgggtt tacccctgtg
     5281 gttctggcgc aatgtgttgg tcgcagcgac cggctggatg gttggcgtca gtgtggcgct
     5341 ggcgggtgct atcggcttta ttggtctggt gatcccacat attctccggc tgtgtggttt
     5401 aaccgatcat cgcgtattac ttcccggctg cgcgctggca ggggcgagcg cattgctgct
     5461 ggccgatatt gtagcgcgcc tggcattagc tgccgcagag ctgcctattg gcgtggtcac
     5521 cgcaacgttg ggtgcgccgg tgtttatctg gttattgtta aaagcaggac gttagccgca
     5581 aaaagacggt ctatgattaa aagctaagat ttttactgac cacacccagg agaaacgatg
     5641 caagattcca ttctgacgac cgtagtgaaa gatatcgacg gtgaagtgac cacgctggag
     5701 aagtacgccg gtaatgtgct gttgattgtc aatgtcgcct caaagtgtgg cttaacgccg
     5761 caatatgagc agttggagaa tattcagaaa gcctgggccg atcgtggttt tgtggtgctg
     5821 ggattcccgt gcaaccagtt tctggaacaa gaaccgggca gcgatgaaga gattaaaact
     5881 tactgtacca ccacatgggg ggtgacgttc ccgatgttca gtaagattga agttaatggc
     5941 gaaggacgcc atccgctgta tcaaaaattg attgccgcag cgccgaccgc agtcgcgccg
     6001 gaagagagcg gattctatgc ccgtatggtc agcaaaggtc gtgcaccgct gtaccctgat
     6061 gatattttat ggaattttga gaaattttta gttggcaggg acggattggt catccagcgt
     6121 ttttccccgg atatgacgcc ggaagatccc attgtgatgg aaagcattaa actggcgttg
     6181 gcaaaataat gtctattgtg atgcagttac aggatgttgc ggaatctacc cgcctggggc
     6241 cgctttctgg cgaggttcgg gctggggaga tcctgcacct ggtggggccg aacggcgcgg
     6301 gtaagagtac cttgcttgca cgaatggccg gaatgaccag cggtaaggga agcattcagt
     6361 tcgcggggca accactggaa gcatggtctg caacaaaact cgcgctgcat cgcgcctatc
     6421 tttcacaaca gcagacgccg ccgtttgcaa tgccggtctg gcactacctg acactgcatc
     6481 agcacgataa aacgcgtacc gaactactga atgatgtcgc aggggcgctg gctcttgatg
     6541 acaaactcgg acgtagcacc aatcaacttt ccggcggtga atggcaacgc gtacgtcttg
     6601 ctgcggtggt gcttcaaatc acaccacaag ctaatcccgc aggccaattg ctgcttcttg
     6661 atgagccgat gaacagtctt gatgttgcgc aacaaaatgc gttagacaaa attctgagcg
     6721 cgctgtgtca gcaaggactg gcgattgtga tgagcagtca cgatctcaac cacacattgc
     6781 gtcatgcgca tcgggcgtgg ttgctaaaag gtggaaaaat gctggccagt ggacgcagag
     6841 aagaggtgct cacgccggca aatctggcac aggcctatgg gatgaatttt cgccgtctgg
     6901 atatcgaagg tcacagaatg ctgatttcga ccatctgatt tcattagtat cttgaaaatc
     6961 gggtaattac gacgctaaat taatgcaaga ataaaaaaca gaggaaccgt acggaatgcg
     7021 tttctgcctt attttgatca cagcactgtt tctggccggg tgtagccacc ataaagcacc
     7081 gccgccaaat gccaggcttt ctgattcgat caccgttatt gccggtttga acgaccagct
     7141 acaaagctgg catggcacgc cgtatcgtta tggtggcatg acgcggcgcg gtgtggactg
     7201 ttcgggattt gtggttgtga cgatgcgcga tcgtttcgat ttgcagctgc cccgtgaaac
     7261 caaacaacaa gcctctattg gcacgcaaat tgataaagac gagttgctgc caggtgacct
     7321 ggtctttttc aaaacgggtt ccggacaaaa tggtttgcat gtaggtattt acgataccaa
     7381 caaccaattt atccacgcct ctaccagcaa gggagtgatg cgttcctcac ttgataatgt
     7441 ctattggcag aaaaatttct ggcaggcgag acgaatctag tatgtcgaaa aatggacggc
     7501 gatgatacat cgccgtccat ttactcctga atacagtagt attttgcaga cagaaattcc
     7561 gtaaatggat tattcgttta taatcaggat aatctaaaaa aaaggacccc tgaaaaagtg
     7621 gttaacatgg aacaaatgaa agttactcct tctgtaattg ttatgtctta tggcctcatc
     7681 ccttttagcc ggatactgaa aaacatcctt cgagagggac ggttaccatg aagatttttt
     7741 tggagaatct ttatcattct gattgctatt tcctgccgat aagagataac cagcaagacc
     7801 ttgtcggcgt tgagttaatt acccatttct ccagtgaaga tggtacggtt cgtatcccga
     7861 cgagtcgggt tatagcgcaa cttaccgagg agcaacattg gcaattattt tctgaacagt
     7921 tggaattact aaaatcatgc caacactttt ttattcaaca taaactcttt gcctggctta
     7981 atttaacgcc acaagtggct acattattat tagacaggga taatttcgca ggcgaactat
     8041 taaaatatcc gtttattgaa ctgttgatta atgagaatta cccgcatttc aatgaaggaa
     8101 aagataatcg cgatctttta tccttatcgc aaatgtatcc ccttgtgtta ggtaatttgg
     8161 gagcaggtaa tagcacaatg aaagcggtgt ttgatggtct tttcacccgc gtcatgctgg
     8221 ataaaagttt tattcagcaa caaatcacgc atcgctcctt cgaaccgttt attcgtgcga
     8281 tccaggcgca aatttcccct tgttgcaatt gcatcatcgc ggggggcatt gatacgccag
     8341 aaatattggc acagattata ccctttgatt tccatgctct ccagggatgc ctgtggcctg
     8401 cggttcctat aaatcagata acgacgttgg ttcagcgata accctcctgt ttgctggtgt
     8461 ttaggacgag agtaaccgtc tacactatca aacaggagga tctatgaccc tgtcttttat
     8521 tacccgctgg cgcgatgaat tgccagaaac ctatacagca ctttccccta cgcctttaaa
     8581 taatgcccgg ctgatttggc ataataccga actggctaac acgctgagta ttccatcgtc
     8641 gctgtttaaa aatggcgcag gtgtctgggg cggcgaaaac ctactgcctg gtatgtcacc
     8701 actggcgcag gtttacagtg gtcatcagtt tggcgtctgg gcgggccaac tgggtgatgg
     8761 gcgcggcatt ttactcggcg aacaactgct tgctgatggc actacaatgg actggcatct
     8821 gaaaggtgct ggcctgacgc cttattcgcg aatgggtgat ggacgggcgg ttttacgttc
     8881 gacgatacga gaaagtctcg ccagtgaggc gatgcattat ctgggcattc cgacgacccg
     8941 cgctttaagt atcgtcacca gcgattcgcc agtgtatcgg gaaacggtgg agccaggcgc
     9001 gatgctgatg cgtgtggcac caagtcatct gcgctttggt catttcgaac atttttacta
     9061 tcgccgcgag ccggaaaagg ttcgtcagtt agctgacttt gccattcgtc attactggcc
     9121 acatcttgca gatgatgagg acaaataccg tctctggttt accgatgttg tcgcacgtac
     9181 cgcctcgtta attgcccaat ggcagactgt cggctttgct catggggtga tgaataccga
     9241 caacatgtcg ctgctggggc tgacgcttga ttacgggccg tttggttttc ttgatgatta
     9301 cgaacccggt tttatttgta atcactcgga tcatcaaggg cgttacagct ttgataatca
     9361 acctgccgtc gcgttgtgga atttacagcg tctggcgcag acattgtcac catttgttgc
     9421 cgtagatggc ctgaatgagg ccctggacag ctatcagcag gttttgttga cgcattatgg
     9481 acaacggatg cggcagaaac tgggcttcat gacggagcta aaagaggata acgcgctact
     9541 gaatgaatta ttcagtctga tggcgcgaga gcgcagcgat tatacccgca cattccgcat
     9601 gctgagtctg accgagcagc acagcgcggc gtcaccgcta cgtgatgagt ttattgatcg
     9661 tgcagcattt gatgactggt ttgcccgtta tcgggggcgt ttgcaacaag acgagattac
     9721 cgataatgag cgtcagcaac tgatgcaaag cgttaacccg gctctggtgt tgcgcaactg
     9781 gttggcgcaa cgggcgattg aagcggcaga aaagggtgat atgacggaat tgcaccgcct
     9841 gcatgaggcg ttgcgaaatc ctttcagcga cagagatgat gactatgtca gtcgtccacc
     9901 tgactggggt aaacggctgg aagtcagctg ctcgagttaa aggctacttg gtcaacagca
     9961 gcttgccagc ctgagtttta cggagcagat attcttgtcc gtcatgatcg ataatcagtt
    10021 tgccatccgg acctaacagt gtctggctgg taattcgccg gatgggctgc ggggaagcgg
    10081 tcttgtgatt ggcatccgtt tcaggcgtga gttttctgct atccgtataa cgcataaaaa
    10141 ttaataataa tttatataac aatgattctt attatcaata ttatggaggg ccagagcaag
    10201 cagtaatttg ttgagaagac aacaagttgc gcagaaatgc ccatcctgtc aggaatgggc
    10261 acgcattcag aagcgggtat ctaccgcaga ggcgagtttt tcgaccaggc gttcggtatc
    10321 ttcccagccc agacacgggt cggtaatgga ttgaccgtaa gtgagcggct gaccgccgac
    10381 gattttttgc gttccttcgc gcaggaaact ttccgccata attccggcaa tcgccgtaga
    10441 gccattgcgg atttgctgac aaatatcctc acaaacttct aactggcgac ggtgctgctt
    10501 ctggcagtta ccgtggctga aatccaccac cagatgttca ggcaaatcaa attcgtgcag
    10561 cgtatcgcag gctgcggcga tatcatcggc atgataattc ggttttttgc cgccacgcat
    10621 aataatgtgg ccatacgggt tgccgctggt ctgatagatg gtcatctgac cgtttttgtc
    10681 tggcgagagg aacatatggc tagcgcgggc tgcgcggata gcatccacgg caatccgcgt
    10741 gttgccatcg gtaccatttt taaaacccac cggacaggag agtgccgaag ccatttcgcg
    10801 gtggatctga ctttcggtag tacgtgcgcc aatcgcgccc caactgatta aatcagcaat
    10861 aaactgaccg gtcaccatat cgaggaactc agtcgcggta gggacgccca gctcatttac
    10921 ctgtaaaagt aatttgcgcg ccagctccag accgtgattt acccgatagc taccgtttaa
    10981 atctggatcg gagatgagtc ctttccagcc gacaaccgtt cgtggttttt caaaataggt
    11041 gcgcattacg atttccagcc gtgactggta ctggttgcgc agcgactgca gacgggtggc
    11101 gtactccatc gcagcggtga gatcgtggat cgagcagggg ccaataatga ccaacagtcg
    11161 cttatcttca ccattcagta ttttttcaat tctacggcgg gagtcggtga catgggtggc
    11221 gacgccaggc gttacgggat accgtagcgc gagttcggcg ggcgttacca ggctctcaat
    11281 acgcgcagta cggagttcgt cagttctgtt cattacaagt ctcagaaatt tgttgcttct
    11341 acggcaggac taccgggaag tgatgcgcat cacgataaac caatcgcagc tgatttcaag
    11401 tgcgaggtgt gtcacacctc gccgggtggt tagcatgata acaaaaaaac aagctaatgc
    11461 actagttctc tagtacattc ggcggctaag gcccatgata tcgaggatct tggtggcaat
    11521 ctcttctacc gaataattgg tactgttaat ccacgggatc tgatttttac ggtacaacgc
    11581 ttccacttcc gcgacttcca tcctgcactg acgaagcgag gcatagcgac tgttctcccg
    11641 acgttcctcg cgaatcgccg ccagacgttc cgggtcgata gtcaggccga acaatttatg
    11701 ctgaagtggt ttgagcgacg cgggtagcac cagattatcc atatcatcgg caataaaggg
    11761 gtagtttgcc gcgcggatac caaattgcat tgccagatac agactggtgg gggttttacc
    11821 acagcgagaa acaccgagca ggatcacctg agcctggtcc agattgcgca acgaaatacc
    11881 gtcatcgtgg gcaagggtgt aatcaatcgc cgcaatgcgc gcatcatatt tattgagatt
    11941 attagggtta aggccatggg tacgatgagc aatcggcgtt ggatccagtt tcatctcttg
    12001 ttgtagtggg gcaaccagcg cctgaacgat atcctggcaa aagccttcac tttgcaagat
    12061 gatagcgcga atctccggca acacgatgga gtagaagacc agcgggcgca cacctgtctg
    12121 gtgataaatt gcgtcaatct gatccttcac tgcccgtgca cggctctcat tttcgacaaa
    12181 cggcagcgta atgctgctga tagtgacggg aaattgtgac attactgcgt gtcctaatac
    12241 ctccgcagtt attgccgtac catcagaaat ataaaaaacg tggcgatcaa cagcattatc
    12301 cattttgttc ttcccgtgat gcagacataa ttctatgaaa gcataaatta aaaacgcaca
    12361 gaagcgtaga acgttatgtc tggtttataa aatgaaactt aaattttatt ttttatgaaa
    12421 acagcatttc atttttatgg tttcgtttat accgatggtt tatgtggaaa ttgtcgaaga
    12481 gagcagattt gcgcaacgct gggatcagtc ttaaaaagta aaaaaatata tttgcttgaa
    12541 cgattcaccg tttttttcat ctggttaaat atgcaaagat aaatgcgcag aaatgtgttt
    12601 ctcaaaccgt tcatttatca caaaaggatt gttcgatgtc caacaatggc tcgtcaccgc
    12661 tggtgctttg gtataaccaa ctcggcatga atgatgtaga cagggttggg ggcaaaaatg
    12721 cctccctggg tgaaatgatt actaaccttt ccggaatggg tgtttccgtt ccgaatggtt
    12781 tcgccacaac cgccgacgcg tttaaccagt ttctggacca aagcggcgta aaccagcgca
    12841 tttatgaact gctggataaa acggatattg acgatgttac ccagcttgcg aaagcgggcg
    12901 cgcaaatccg ccagtggatt atcgacactc ccttccagcc tgagctggaa aacgccatcc
    12961 gcgaagccta tgcacagctt tccgccgatg acgaaaacgc ttcgtttgcg gtgcgttcct
    13021 ccgctactgc agaagatatg ccggacgctt cttttgccgg tcagcaggaa actttcctca
    13081 acgttcaggg tgttgacgcc gttctcgtgg cagtgaagca tgtattcgct tctctgttta
    13141 acgatcgcgc catctcttat cgtgtgcacc agggttacga ccatcgtggc gtagcgctct
    13201 ccgccggtgt tcagcggatg gtgcgctccg acctcgcatc ttctggcgtg atgttctcca
    13261 ttgataccga atccggcttt gaccaggtgg tgtttatcac ttccgcatgg ggccttggtg
    13321 aaatggtcgt gcagggtgcg gttaacccgg atgagtttta cgtccataaa ccgacacttg
    13381 cggcgaatcg cccggctatc gtgcgccgca ccatggggtc gaaaaaaatc cgcatggttt
    13441 acgcgccgac ccaggagcac ggcaagcagg ttaaaatcga agacgtaccg caggaacagc
    13501 gtgacatctt ctcgctgacc aacgaagaag tgcaggaact ggcaaaacag gccgtacaaa
    13561 ttgagaaaca ctacggtcgc ccgatggata ttgagtgggc gaaagatggt cacaccggca
    13621 aactgttcat tgtgcaggcg cgtccggaaa ccgtgcgctc acgcggtcag gtcatggagc
    13681 gttatacgct gcattcacag ggtaagatta tcgccgaagg ccgtgctatc ggtcatcgca
    13741 tcggcgcggg tccggtgaaa gttatccatg acatcagtga aatgaaccgc atcgaacctg
    13801 gcgacgtgct ggttactgac atgaccgacc cggactggga accgatcatg aagaaagcat
    13861 ctgccatcgt caccaatcgt ggcggtcgta cctgtcacgc ggcgatcatc gctcgtgaac
    13921 tcggcattcc ggcggtagtg ggctgtggtg atgcaacaga acggatgaaa gacggcgaga
    13981 acgtcactgt ttcttgtgcc gaaggtgata ccggttacgt ctatgcggag ttgctggaat
    14041 ttagcgttaa aagctccagc gtagaaacga tgccggacct gccgttgaag gtgatgatga
    14101 acgtcggtaa cccggaccgt gctttcgact tcgcctgcct gccgaacgaa ggcgtgggcc
    14161 ttgcgcgtct ggaatttatc atcaaccgta tgattggcgt ccacccacgc gcactgcttg
    14221 agtttgacga tcaggaaccg cagttgcaaa acgaaatccg cgagatgatg aaaggttttg
    14281 attctccgcg tgaattttac gttggtcgtc tgactgaagg gatcgcgacg ctgggtgccg
    14341 cgttttatcc gaagcgcgta attgtccgtc tctctgattt taaatcgaac gaatatgcca
    14401 atctggtcgg tggtgagcgt tacgagccag atgaagagaa cccgatgctc ggcttccgtg
    14461 gcgcgggccg ctatgtttcc gacagcttcc gcgactgctt cgcgctggag tgtgaagcag
    14521 tgaaacgtgt gcgcaacgac atggggctga ctaacgttga gatcatgatc ccgttcgtgc
    14581 gtaccgttga tcaggcgaaa gcggtggttg aggaactggc gcgtcagggg ctgaaacgtg
    14641 gtgagaacgg gctgaaaatc atcatgatgt gtgaaattcc gtccaacgcc ttgctggcag
    14701 agcagttcct ggaatatttc gacggcttct caattggctc aaacgacatg acgcagctgg
    14761 cgctcggtct ggatcgtgac tccggcgtgg tgtctgaact gttcgatgag cgcaacgatg
    14821 cggtgaaagc actgctgtcg atggcgattc gtgccgcgaa gaaacagggc aaatatgtcg
    14881 ggatttgcgg tcagggtcca tccgaccacg aagactttgc tgcatggttg atggaagagg
    14941 ggatcgatag cctgtctctg aacccggaca ccgtggtgca aacctggtta agcctggctg
    15001 aactgaagaa ataaaataaa tacccggcgg cgttttgtcg ccgggtttat gtgatccccg
    15061 aagatgaaac ttattcaatc tcttcacaga catcctgagt taatcgccgc ataatatctt
    15121 tccttaacaa aaacttttgt attttacctg agacagtgcg cggtagtttt tcgattacca
    15181 cgatatgttc aggatattta tattttgcga cccgtttacg gctaaaaaaa gccaccacct
    15241 cttccagcga taatgaatga tgcggcgctt tcagcacgac ataagcgcat gaacgttcac
    15301 ctaaacgttc atcgggcatc gcaaccacac aggcatcgtg aattttagga tgctgcaata
    15361 aaatatcttc tacttcacgg ctgctaatat tttcgccgcc gcggacaata atatctttct
    15421 tgcgcccggt tatttttata tagccagcct catccatgcg gcagagatcg ccgctgtaat
    15481 accagccttc ttcatccagc gcacgggcgg ttaattcagg ttcatcaaaa taccccataa
    15541 acacattggg gccacgcgag gcttcttcac cttcgcaacc tggcggtaaa gtcttgcgtg
    15601 cgtcatcgac cactttaatc tctacacctg cggcagcgta accgtcggtg tgcataaagc
    15661 gcggcaaagg atcgtcgaga ttcaccaccg catgcggcga actttctgtg gaaccataaa
    15721 cacttaataa tttaatgccg cgctgctggc attctcgcgc cacttttttg gggattgtgg
    15781 tacctccgca aagaaagaag cgcagcgctg aaaggtctgc gggttgtttc tctagtaaat
    15841 tcaaaagatc atagacaaac ggcgttgcgc cgagcataca ggtgcaacgc tgctgctcaa
    15901 gcagcgcgag acacgcatca ggagtgaaaa tatctaacaa cacgctgcga gcaccaatca
    15961 aaaaaggtgc tgttacgcca tgcagaaagc ccgttgcgtg accaagtggc gcaggcatca
    16021 taaagacatc ctgccaggtc agatttagcc gcgcgcaata agcccgctca ctggcgagaa
    16081 tattgttatg cgttagcatc acaccctttg gcagaccctc ggttccggag gtaaatagca
    16141 cagcggctaa ttcatcgccg tgggtcgtta tcgccgtggt cagaggagta ttgtcggcga
    16201 gaatctgact taatgagagg gaagaggtgg cgggagccag tttgtccacg ccgacaattt
    16261 gttgtagttg tggaagctga ttttgcagcg gcaggattaa atctaccgga cgcgtttgtt
    16321 taaacaacgt cggtgcaaag aacatttttg cctgacactt attgagcacc cacaccagtt
    16381 ctgcttcccg ccaggaaggt aacagcggca cggaaaccgc accggttttc aggcaggcaa
    16441 gatagataac ggtaaattca caccagcctg gcagttgaaa tgcgatgcga tcgcctgatt
    16501 caataccctt cgctaacatc cagtttgcca gacagctcgc ggcgtgatcg agcgcgctat
    16561 aggtgtacgt tgcaccatga ttatcgacca cggcaatttt gtctggcatc gcacgagcgg
    16621 tctgctgcca gtaatcggcc agcgaagcat cgccccataa cccttgctga cgatacgccg
    16681 cacgacgttg ttcgttgaac gttaatgtca ctttcatttt ggactctcga aacagaacat
    16741 caggcccgag atgcgggcct gtgggatgca tgattagccg tagcgaaatt caataccgaa
    16801 ggtgcctgcg ggatattgcc actgttcgag aatagtgttg ccgcacagca cccgacaggt
    16861 gccgcactcc agacaaccgg cggaatcgaa atgaatgttt cctgcgtcat cctgcttata
    16921 aagtccggca gggcaagctt tcattaattt acggaattca ttgatatcgg gatttgccgc
    16981 caaaatgata tgcgggtggc cctcatcaac atggaattta tcgacgccta atttgatgtc
    17041 gacgttaacc gtagcgttct ggctcatagc gcggttgctc ccttaatgcc atctttcagc
    17101 aagttgatca gcccaatttt cttcgcgtgt cccatgatca ttttgcgtac cggctggttt
    17161 ggtttgccgt caatggtgaa catatcgttc atgatgtcgg cgaccattcg tgggtattgg
    17221 ctaaacaggc gcgggttttc catcagcgcc gggatcttgc ggaaatgctg catatcgcgc
    17281 ataacacaac tttgttccag ctcacgtttg tattgcgcca gactgctggc ggagaaatcc
    17341 gcgcgttctt tggcggcgat caccgttgtg gcggcagcct gagctgatgc aatggctaaa
    17401 tccatgccgc gaactgtaaa acccaaattc aggcagaagc ctgcggcgtc accaacgatc
    17461 atcacgccat cgttaaccag ctgcggcacc attgccagac cgccctccgg caccatatgc
    17521 gcggaatatt caagcagttt gccgccgcta atcagcgggc gaatagcggg gtgttgttta
    17581 aaatcttcca gcatttgcgg cacgcttttt tgcgcatggg cgatatcacc caatccacaa
    17641 accagcccca acgatacgga atccttgttg gtatagagga atccgccgcc catcaggccg
    17701 tcagaagggg aaccggcaaa cagccaggcg gcaccttcct cgcccgtaac attaaagcga
    17761 tcgttgatct gttctggtgt caggcctata acctctttaa caccaacagc gtaatgatgc
    17821 ggattggaag cggaaaccat tcccagcgag cggccaagca tcgagttaac gccatcagcc
    17881 agaatcacca cattcgcttc gagaatatca tccccggcct ggacgccagt gaccttgttt
    17941 ccttcacgaa ccagcgcatc gacgcgtacg cccgggataa actgcgcgcc cgcctgctcg
    18001 gcttgttcca tcaaccacgg gtccagacga ttacgcaata cggtataaga cgcgtgttgc
    18061 ggaacatctg gttgctcgcg gtgaaaatcg agggtaacgg cgctttcttc ggttaagaag
    18121 gagattttct cgcgtgtgac cttgcgttct accggcgctg atgctgcaaa gcctggaata
    18181 attgcttcaa gtgtgtgggc ataaagacgc ccgccggtca tgtttttaca tccggcactg
    18241 tcgccgcgtt ctatcaccag gacatccagc ccggctcgcg ccatgaccag tgcggcaacg
    18301 ctaccggcaa cgcccgcacc gaccacaatg gcatcaaatt tgtcatccga catgcgttat
    18361 gctcccttcc cgctgcgttt acagcgggag atgtggttga aagggaatta gcgattcaac
    18421 tggctaatca gggcagggac gactttgtag atatcgccca ccagaccgta gtcggcatag
    18481 ttgaagattg gcgcattttt atctttatta atggcgacaa tcacttttgc gccgttgccg
    18541 ccaaccatat gctggatctg cccggagatc cccagcgtca ggtagagatc ggatttcagc
    18601 aacacgccgg agacaccgat ataacgttca cgctccatcc agttctcgcc ttcggcaatt
    18661 ggacgtgaac agccgacttc agcattcagc actgccgcca gttcgtggac cattttcagg
    18721 tcatcctgcg ccgccagacc acgaccgacg ccaaccacac gttttgcttt gctcaggtcc
    18781 acgctgcttg ccgctttcgc acggcgttcg cgacagagaa tttcatgacg cggggcaaca
    18841 taaggtaccg tttctgtcgg gcactgatga gagatatcac gggtgcacgg ttcctgaaca
    18901 ccgggtgcaa gggtaatgat cgccagcggg ctgttgatct tttcctgagc gaacgctaac
    18961 ccgccataca tccggtgttc ggcgcaaatg tgaccatcga caatatccac cgccgtggca
    19021 tcgttcacca gcgccgcatt cagttgcaca cttaaccgtg cggccagagc tttaccacgt
    19081 ttcgtcgcgg ccaacagcaa catagcgggg tgtttatctt tcagcagggc agcaatgctt
    19141 tcggcgtaat tttcagtacg ttgcagcgcg tcgttttgct caagaacata aatgcatttt
    19201 ggcccataag gcataactgc ctgcgcctgc tcggtatttt gtacaatggc atacacctgt
    19261 tggccccatt gctgagcgct gccaaacagt tcagcataac gttcaggatt atcgctaaag
    19321 acccagacgc tgtttaattg actcatcgtt tcttctccgt aggcttcagt tcagggcttt
    19381 cttcagatgt tccgccagct cggcaatggc ctccggcgaa tcgttatcga gaatgatgtg
    19441 cttacgttct gtttgcggcg gtacgcgaat gccggtcagt tcagcaagtg gtgcgctctg
    19501 gctccagcca atatcacttg cctgccactg atttaccggt tttttacccg cgccgagaat
    19561 ggctttcatc gaaggaatac gtggcacgtt aatatcggag gtgacgcaga gcacggctgg
    19621 aacagagagt tcaataactt caacatcatc ttcaagcgtg cgttcaatca ccagtgtatt
    19681 gccctgacgc tgaatagcac tcactgcatt aatcaccgga agttgcagaa tttctccgac
    19741 cagcaagcca acctgctggg cataaaggtc gccggaacct tcaccaaaga tcagtaaatc
    19801 gaagccgatc ttttcaactg ctgtcgccag cgcctttgcg gtatcgagag gcagtgcatg
    19861 ctcaagttgt gcatcctgca ccatatacag gctgtgcggc ccgcgggata gcacgtcttt
    19921 gcgcactttc gagttctgca acaaagagcc gccgacggtc agcgcggcta tctcatcgtc
    19981 atctgttgca agctggcttg cagcttcaat ggcattgaga tcgaactggc tgattttggc
    20041 gtcggcattg tcgaaattca gggtgtattc tggagtgaca acaatgtcct gttcttcagg
    20101 caccagctta aagcaggtta ttattttcat gatttctcct gtgagtcgca tacggaacca
    20161 tctccgatgg aaagtaaaaa tggcctgaaa atagtgtgcg atagcggtgc ggggttttcg
    20221 acggagcaag ctgttttcct tgccaacaat cttgcaaaaa aaatgcgatc gctaaaacaa
    20281 aagtgcaatt tgcggcaata tagttattcc atgaccgtac tatcttgcat tcagtgtaaa
    20341 taatcttgtt tagctctcgc taaacgcggt tgtataaccg caatacacgc cgtattttat
    20401 ccggcatata ttgtttttgc ttgctcatgg aatagggagg ttgtcatgta tcaacgctgt
    20461 tttgataatg ccagtgaaac gctgtttgtc gccggtaaaa cgccacggct ttcacgtttc
    20521 gcatttagcg atgatccaaa atgggagtct ggacatcacg ttcatgacaa tgaaaccgag
    20581 ctgatttacg tcaagaaagg ggtagcaagg tttaccatcg attcttcgtt atatgtcgcg
    20641 catgccgatg acattgtggt gatagaacgt ggcaggttgc atgcggtggc ctctgacgtt
    20701 aacgatccgg caacgacgtg tacctgtgcg ctgtacggct ttcagtttca gggggctgag
    20761 gaaaatcagc tactgcaacc gcattcttgc ccggtaattg ccgcaggaca gggaaaagaa
    20821 gtcattaaaa ccttatttaa tgagctaagt gtgattttgc cgcaaagtaa aaatagccaa
    20881 acatcttcgt tatgggacgc atttgcctat acgttagcaa ttctttacta cgaaaacttt
    20941 aaaaatgctt atcgttcgga gcagggatat attaaaaaag atgttctgat aaaagatatt
    21001 cttttctatc tgaataataa ctatcgcgaa aaaatcactt tagagcagtt atcgaaaaaa
    21061 tttcgtgcca gcgttagtta tatctgccat gaatttacca aagagtatcg tatttcccct
    21121 attaactatg ttattcaacg gcgtatgacg gaagcgaaat ggtcactcac taatactgaa
    21181 ttatcacagg cagagatctc ctggcgtgtg ggttatgaaa atgtcgatca ctttgccaaa
    21241 ctgtttttgc gccatgtcgg ctgttcgccc agcgattacc gcaggcaatt taaaaactgt
    21301 tttgcggaac aagaaatcct atctgaattt cctcaaccag taagtcttgc cggataaatc
    21361 tggcggcgca gtttgttaag aagctgcgcc gcctgcagat tatttgttct gataatcttt
    21421 caggatctgc cgacctgcta cgtaaatcat aatttcgtct gtaccgccgc cgatacgttc
    21481 acaacggaca tcacgccaga agcgggagac gcgcgcctca tcggtatagc ccagaccgcc
    21541 catgatttga atcgcatcat caatgacttc cattgcggta cgtgcgcagt acagttttgc
    21601 cagcgccgcg ctggtgcgca gtgactgatg ctgatcggct tgccatgcca ctttcaacac
    21661 catgttgcgc atgttgtcga tcttaatcgc catcagcgcc agtttttcct ggatcatctg
    21721 gttatgacca atgggcttac caaaagcgat acgctggttg gcatagcggg cggcatcttc
    21781 gaatgcgcat tcggcaaagc cggtgctgcg cgcggcgttg atcaggcgct ccatctcaaa
    21841 gttgtacatc acattgagga aacccattcc ttcttcgccc accatgtcgc tctcttcaac
    21901 ttccacgttg tcgagataga cttcgcaggt gctgagcata tgccagccga ttttatgcag
    21961 tggattgatt ttaatgccgg gcttactgga gtcgacccac cacagagtaa aggccttttt
    22021 cggatctttc ggttgcggat cgcgcgccaa caccagcata tatggatact ctttcgcacc
    22081 ggtaataaag gttttctgtc cgttgatgta aaccttgccg tttttacgcg tgtaagtggt
    22141 agtggcactg ttgttgtctg agcctgcgcc tggctccgtc aatgccaggg cataggcggg
    22201 atcacccgtt tccagggtgc tctctgccgt tttacgtagc tgctctgcag aaccgaaacg
    22261 gcgcatactg tgaatacatt gaccgttggt aatcaaaaat gccggagcac cgcattttga
    22321 cacttccatc agcgccagca tttgggtgac gtaatccgca gggataccac caaattcttc
    22381 cggcacgcca agcatggaaa taccgttatc cgccagtgcc cgcataaact cacgcgggta
    22441 tgtcccgttt tgatcgcagg tgcggaaata ctcttccgga aagttagtcg taatcagttc
    22501 gcgaatactg gccagcagca gttcttgttc ttcagttaaa gagaaatcca tcattatact
    22561 cctgttaatg agccgcttca ggcaggacaa aacccatcgc cgcatcgata aataatcttt
    22621 cgtccatcag tttgagttct ggcgaaatca ctggggtgaa atccattttg tcgagaatat
    22681 ctttttgtaa atcgacgcca ggagcgattt cgattaaatg caggccgtct tcttttagcg
    22741 tgaatacggc gcgctcagtg atataacgaa catccagccc tcgctcgaga gcgatttttc
    22801 cgctgaaagt tatttccggt agttcccgaa taaatttctt cacccgtcct tcctggacga
    22861 tatttaattt gccgtcggta atttctgttt ttaaactgcc cgcagttaat gtgccgcaga
    22921 aaacgatttt cttcgaagtg gcactgatat caataaatcc accggtgccc atgattttac
    22981 cattgaattt atgcacgccg acgttaccgt gctggtcgac ttcagcaaaa ctcaaataac
    23041 aaacatccag accaccaccg tgataaaaat caaactggga cgtcatatcc agaatggcgc
    23101 gggtattcac gttcgcgcca aaggcgatcc cctgtgaagt aataccgcca atcggacctg
    23161 tttctaccgt cagaataaag tcatcagcac aaccttcttc ccgggcgacc aggccaatgc
    23221 cgtcagcaat accgacgccg acattcccca ctgcgccttt gcgcatttcg aataatgcac
    23281 gccgtgcaac taatttacgt tggtttaggg gcaggctaag tttggtgctg tcatcaaggg
    23341 tgaagtcacc agaaataaag cggttaaccg gcgcaccgcc atacagttgg gtttgatccg
    23401 gatcgaccac cacaatatcc accagataac ccggaatacg gacagattta ggatgcagcg
    23461 tggctttctt aaccattttc tgcacctgca tcatcacaat accgccgtta ttgtgcaccg
    23521 cctgggcaat aaccaatgcg tcgagataca tcacctcatc ttcaaaagtg gcgtagcctt
    23581 cgctgtcgca ggtggtagcg cgaatgaagg cgatatctgg cgcaatcgct ttgtaataga
    23641 gatattcttt gttatcaaac tcgaccagtt taatcaggtc ttctttagtg acttcattca
    23701 gtttgccgcc ttgctggcgt ggatcgacaa atgttccgat gccaatatca ctaataatac
    23761 caggctggtg ggctgcggcg gcgcgtaagg tttgtgtaag tacaccttgt ggatagttat
    23821 aagcaataat tttattttgt tctgcgagat cagaaatacg cggtgattgt ccccagtgac
    23881 cgcataatgc ccatttcacc aggccttctt gcgccagcgg actaatacca cggtcggcgc
    23941 gatcgccaag ccctgttgga ctaataatcg ataaattacg tggtgtttga gtctgtttat
    24001 atttatcagc aagagcagta attaacgtgg tggcttccag aataccgccg ccagcgccta
    24061 acacacaaag tgttgcttcg tcgggaatat aattcaccgc ttcctgtgcc gataggaccg
    24121 gcactcgtcc attaatacga ggtggtttta caggtttcat tgttaattcc tgattcaggt
    24181 gtgattttcc ctacgccaga tggagaatta gcgcacagag actcacgaaa tatttttatt
    24241 aaatacggtt aaaaatactt tttttagttc ggcgggagag ggtattcccg ccgaaaatat
    24301 tattgcttat gcctgatgta aaatagttaa taccgtacgc aaatcattta ccgagatttg
    24361 ccctggcgca gaggcttttt ttaccgcacc aaaagttgcc gccgacccaa atacttcacc
    24421 agccagacga gaaattacgc cagtttttgc catcgacatc gtgatgattg gacgatcggc
    24481 atactgctcc tgcatctcca gggtcgcggc aagcaacgtc agcacatcgc tggtactttg
    24541 cggcatcagc gcaatcttag gaatatcggc gtcgaaggac tgcattttgc gcagacgggc
    24601 aatgatttct tcggcttccg gcgttttatg gaagtcatgg ttggacatga caactttcac
    24661 atcatgcgcg tgggcgtagg cgacggtttc tttaacctga tcatcgccgg taaataactc
    24721 cagatcgatc atatcaacca ggccgctgtc gatggctgca cgattgagtg caatataagc
    24781 ctcggtggaa atcgcctgct cgccgccttc tttggcactg cggaaggtaa acagcagcgg
    24841 tttttctggc atggtttcac ggagaatttt tgctgccgcc atgacagact ccacattgga
    24901 gaggtcggca tagtggtcca cacgccattc cagaatatca aagtccgctt cacgataggc
    24961 gagagcttcg gatttcacgc tggcgatatc tttcgccatc agcgagacga tgattttagg
    25021 tgcgcccgta ccaatgacga gatcttttac agttacggtt ttcatttttt accctttctg
    25081 cacgcggtca gcctgtcagg caccgaaccc catgacctgt ttaacatatt ccagagggaa
    25141 atctttgcca gtccataacg tgaactgttc agccccttgc cacaacaaca tgccgtatcc
    25201 atcaatcgtt ttgcaacccg cttgttgcgc ctgctgcaat aacttcgtca tatgagggtt
    25261 atacacgcat tcagtgacca gaagtcccgg gtgtaacaga ctgatatcat taaccaatga
    25321 ttcattctca aggggtttca tacccacttt ggtgccattg gttaaaatgt cggcggaagc
    25381 cagggcttct gcaaaggctt gctgatcggc gagatcggtg actgtcacga cgcaatcggt
    25441 gttttcgtta acccgctgcg cgaaggcgag agctttatcg aagaactcat cccgacggtt
    25501 aaagagttta atttctttta aaccttcaat tgccccctgc gcgccaattg ccgttgaggc
    25561 accaccggcc cctaatagca ccatcgtttt gcctttgata tcaaaaccgc tctctttaat
    25621 ggcgcgaata tgacccgtgc cgtcggtgtt atagccacgc agatagccat catcatttac
    25681 gatggtgttg atggcaccca ccagtttggc cgctggcgtt aattcatcaa catattcaca
    25741 cgccagttgt ttgttcggca ttgatacacc agttccgcgc attttgaggg cttttaatcc
    25801 ttcaattgct ccgggaaaac tatcgttatc cacttcaaag gccatatagg taaatggcaa
    25861 tcccgctttt tctaaggctt tattctgcat ttcgggcgat aaactgtggc ggatgggata
    25921 ggccatcaac ccaatcaatt cgtattttgc ggtaacatcc ataattaact cctttaacct
    25981 ctatacttaa ttgtgtttaa ccgggtgcta aatttatgct cgccaaaccg cacatcattt
    26041 tcaggaataa tgaaaacgcg gtaatagcgg ataaaaacaa taattgcggt aatcagggcc
    26101 agcagcgcga aagtaaaatc gagaacaatg atatattgca ggccgatgtt cgacagataa
    26161 ccggtaatca gcggaataac aaagttagcc agtccaccca tcatcatata tatactggtg
    26221 actttggctt tgctttttgg gaaaaactct gacatgaccg aaacgccgag ctgtaaaatg
    26281 ccgccagctg ctgagaaacc gataacaaag gctccggcat tacacaccaa tggagatggg
    26341 tacaggtaga taatggcagc tgttattgtt gccagtgcag agttaaatac attagcccag
    26401 atgggccgga ccattttttt cagcagtgcg gcaaaaataa agacacagac cagcgaaccc
    26461 atactgtaat aagagatggt ttttaatgcc tcagcttctg acataccagc aaaagccatc
    26521 gcatatttgg gcatccacac cacaatcaca taaaaggtcg agaatgcggc tacaccgaac
    26581 agtactgatg aaacaccttc cagccagact aacggtttgc tgttcatttg cggtaattca
    26641 ttagctacgc tggcgtccac caactggctg gggaatttgc ttttcaacag catcagcgtg
    26701 atcagtacaa acagaatacc cggaataatc agcccatagc cgtaccagat gttattgagc
    26761 aacatatagc tcaccagcat tggatagaac atttgcccga atgacaccat cgctttaacc
    26821 agtatgaccg ccgaaccaga ggctttcgga aagcattcca tgagcgcggg gtagccaccc
    26881 gtatccagcg ctgagttagc gatacctacg cacactgcca gacagtaggc gagagttaaa
    26941 ttcgggcaag caggaatacc aaagaagaat agcagataca ttattactgc cattaatatc
    27001 accgcccgac gaccaaactt atcggagatc actccgaaga ataaaatact gatcaatcgc
    27061 cccaatccga taccggaaat taaataggca atgcccgcgt tgtcagtgga aaacttttct
    27121 gccaaagatg tcatgttttg ggcaagcgta ataacactaa tgccgtgcag gaaatagctg
    27181 aagtaaatac aaagaacagc caggataaat ggcgtgctga aagccttatt ttgagacatg
    27241 ttcacagctc cttgctgaaa gataatttcg ttaccgtacg tattgttctc cgacggttta
    27301 cataattact atactgaaga tgattttccc tgggtacatg gcataaagtt tatgtttcca
    27361 ggaatattgc atgtcatagt ttgtttttga tcgctatttt ccttagttag cgcagaaaga
    27421 gttatttatc cgtaagatct tgcataacct gaaaagaata ttgcattacc cacccggagc
    27481 gatattttcc tttagcgtgt gatgccgcgt ttctttgcga ctgcgtaagc caataaacag
    27541 tgccagtata aagccgatag cagcgatggc ggtatcgaac cacataatat cggcaatact
    27601 tctttgcgaa agatgagccg taatcaacgg aatggtaaat gttgcaatgc tgcccgcact
    27661 gtaatagatc cccgtagctt tacctttagc gtagggaaaa cgttccgcca ttaacgtcag
    27721 gccaatttgt acaacaccgc ctgcggaggt aaaaccgatg acgaaagcaa atattatcac
    27781 cacataaaat gtgggatgca ggcagacggt gagcagggcg ataaatgaga taaaggtgta
    27841 cagcatcagt aatgttgttg ggcgaacggt attacgaatg agtggagcag taataaatac
    27901 acacagcagc gaacccacgg tgtagatact gagtaatttg atcgacatgg tgtatgacat
    27961 gccagcgaca aattgtccgt attgtgccag ccactggcta accagataaa acgttgccat
    28021 tgagatatag ccatataggg aataactggc taaatcgata attgaacagc gatgttccgt
    28081 agagctggtg gttttcttta tgacaggtaa gtgacgaccc ggatggggtg ggaacgtaca
    28141 acggtataaa aacagagcgt taataaacat aatgcctgcg gcaatcataa aggaccaacc
    28201 gaaccacagt tcagcccaca ctaacaggct gataattagc ggtaataaaa attgtccgct
    28261 ggaaacaaat gctttaatta aaatattggc tgtacccggt gagcgtggaa aagcttccat
    28321 caaactggga taggtgcctg catcgagaaa actgtttgcc attcccgcca gaaagccaaa
    28381 aacataagcg ataatgatgt tattggtgtg caggatgcca aaaaagaagg ccatatagca
    28441 gcacatcccg agcatgataa aagggcggcg accaaagcga tcggataata atcctgcaaa
    28501 aagcaagaca cttaatcgac caatgcccag tgatgagata actatcgaga cgcccgcggc
    28561 attagtctgc caaagcgtct ccagcgaggc catattcagg ctcatcaaaa tgacgcccat
    28621 accatgcacc aggtaattaa aatacaaccc aagtgcggta gggtaatagg gatttttcat
    28681 aggttctact tagcaaaggt gctttaaaaa gtgtagcgat gttgtgtcag tatccgttca
    28741 ttattgttgt aacaacgaca attcctttct gtggagaaat taacaaagcc gctccagatc
    28801 gtgtgcatat aattcagcgg ctaccgattg atagatcttc cagtcaataa aattgccatc
    28861 agggtagact tgttgaaacg cggttaagtc ggggaaaaaa cgcatggtgt tgttgccgat
    28921 gcggttattt attttctcta tgattgcatg aagatgtttt tttcgttgct gacgcatagc
    28981 caacagttgc tcgactacat aatcaggaat ggcgcatttg ccattttccc actgacgcca
    29041 gctttcactg ttaccggtct gggcaatcca ggtcgcgcat tcatcaatag tcatggcaaa
    29101 aatatggcga agtgcctgga gttcataagc gttcatcgtg taatggctgc atgaaataaa
    29161 caataaaata taaataacat tatcagcctg ctgacggctt gaagagagtc acagtatctt
    29221 gtgcaacatt attggaaaga gtgaggggcg aggtagcgaa gcagaaaaat gttaaaaaaa
    29281 agcccatcgt gggagatggg caaagactac acacagcaat tcgttgtttc actcagggga
    29341 tttccatgct tataaatcaa tatgttgatt tataaccgtg agctaatagt aggcatacgg
    29401 actttttgcg tcgatcagat tcgtctcatt agtttaaaaa ctgtaaagaa tttgcgacag
    29461 gtactaccag ctgaatagcg gtaagcagga aaatatgggg aagagcaagc aaatatcaat
    29521 tagcaggggt taacaatcag gcggcagagc cgcctgatga gaaattactt attcgatttc
    29581 tcgatttcgc caagttcttc gagaatttct tccggttggt ccgtcggcgg tgggacttct
    29641 tccacccacg cggcaaacag acgccaggaa acggctaaca gaaccggacc aataaacaga
    29701 ccgatcatcc cgaaagcaat caaaccacca ataacgccag agaggatcag gatcagcggt
    29761 aaatcggcac ccatgcgaat taacattggg cggatgacgt tatcgagcgt gccaaccaca
    29821 ccgctccaaa ctaacaatac cgttccccag gtggtatcgc cagtccagta gagccagata
    29881 atcgccggaa tcagtaccgg caacggtcca agctggacaa ggcaggagag gatcattaac
    29941 accgttagca aagttgcata aggtacgccg gatacggcga ggccgatacc gccaagcact
    30001 gcctgtacta acgccgtcac caccacaccc agcgccaccg cgcggatagc ctgtgccgcc
    30061 agcagcacgg cggcatctcc gcgaacgcct gccagacggg ttgcaaaatg gcgaatgcct
    30121 tgtgccacct gttcaccgcg ccagtacagc agtgcgctga agagaagcat cagcgcacaa
    30181 tgcaccataa agcgcccgat atgcgccgcc tgcccaacga accaggtggt ggtggtgcca
    30241 atataaggtc ggactttcgc catgatcgcc gtccccccca tatccagcaa gttgtgccag
    30301 cctgcataca gcttcgcgcc aatcaccgga atggtattaa gccacgccag atcgggtaac
    30361 gtcatgtcac cactggaaat ggctttaatt agcgggccgc tgccgtcgac gatactgtta
    30421 accagcaaag caatagggat gataaacacc atcactaata acagcgtcat caccagaacg
    30481 gcgagagagc ggcgaccaaa catgatcttt tgcaaacgta acaataccgg ccaggtggcg
    30541 ataaccactg taccggccca tgcaaagccg agaataaaag gttgtacaat ccacagacat
    30601 gccacaatca tgatggctaa aaacagcacc gaaagcagaa tttgtgcgac atccctgggc
    30661 tgacgaacat ttaccataac tacttttcac cttttttgtg cgtcaaatcg tcgacgcgac
    30721 gtgaacacgc gaaactcacc tgcataatga tgagcaattt cagcggtttt taacaggtcg
    30781 attctgccct taattctgat gggcgtgtta gaaaaaaatg tggtacaaca actcaggcaa
    30841 cacgcaaacg attaatctct catcacagac cgcaggaaag ggtcaatata atgattccac
    30901 agatttccca ggcacccggc gtcgttcaac tggtgcttaa ttttttgcaa gagctggagc
    30961 aacagggttt taccggcgat acggcgacaa gttatgccga tcgtctgaca atgtcgactg
    31021 acaacagtat ttaccaactt ctccccgatg cggtggtatt tccgcgttca accgcagatg
    31081 tagcgctgat cgcccgtctt gccgcgcagg aacgctattc atcgctgatc tttacccccc
    31141 gcggcggcgg cactggcact aatggtcagg cgctcaacca ggggattatt gttgatatgt
    31201 cccgccatat gaaccgcatc atcgaaatta accctgaaga gggctgggtg cgcgttgagg
    31261 ccggggtgat taaagaccaa ctcaatcagt acctgaaacc ctttggctac ttttttgcgc
    31321 cagaactttc gaccagtaac cgggcaacgc tcggcgggat gatcaatacc gatgcatccg
    31381 gtcagggatc gctggtctat ggcaaaacgt cagatcacgt acttggcgta cgcgcggtgt
    31441 tgttgggggg cgatattctc gatacgcaac ctttacccgt cgaactggcg gaaacgctgg
    31501 gtaaatccaa taccacaatc gggcgaattt ataacacggt ttatcaacgt tgccgtcagc
    31561 aacgccagtt aatcatcgac aacttcccca agcttaaccg ctttcttacc ggttacgatc
    31621 tgcgtcatgt ctttaacgat gagatgaccg agttcgacct gacgcgcatt ctgacgggtt
    31681 cagaagggac gctggccttt attaccgaag cgcgactgga tattacgcgc ttgcctaaag
    31741 tacgccgtct ggtgaacgtc aaatatgact cttttgactc cgcgctgcgt aacgcgccgt
    31801 ttatggttga ggcgcgcgcg ctttcggtag agacggtgga ctcaaaagtg ctcaaccttg
    31861 cacgggaaga tattgtctgg cattccgtca gcgagttgat taccgatgtg cctgataaag
    31921 agatgcttgg gctgaacatt gtggaatttg ctggtgatga tgaggcactg attgacgagc
    31981 gggtaaatgc actctgtgtg cggcttgatg agctgattgc cagccaacag gcaggagtga
    32041 tcggctggca ggtgtgccgc gagctggcgg gtgttgaacg tatctatgcg atgcgcaaaa
    32101 aagccgttgg tctgcttggc aatgccaaag gtgctgctaa gccaattccg tttgctgagg
    32161 atacctgcgt accgccggaa cacctggcgg attatattgc tgaatttcgc gcgctgctcg
    32221 acagccacgg cttaagctac ggtatgttcg gtcacgtcga cgcaggtgtc ttgcacgtcc
    32281 gtccggcgct ggatatgtgc gatccgcaac aagagatttt gatgaagcaa atctctgatg
    32341 atgtagtggc gcttaccgcg aaatacggtg gtttgttgtg gggtgaacac ggcaaaggtt
    32401 ttcgcgctga atacagcccg gcgtttttcg gtgaggaact ttttgcagaa ctgcgtaaag
    32461 tgaaagaggc atttgacccg cataaccgac tcaaccccgg gaagatttgc ccgccagaag
    32521 gtctcgatgc gccgatgatg aaagtggacg cggtgaagcg cggtacattc gatcgacaga
    32581 tccccattgc ggtacgccag cagtggcgtg gtgcaatgga gtgtaacggc aacggtttat
    32641 gcttcaactt tgatgctcgt agtccgatgt gtccgtcgat gaagatcacc cagaaccgga
    32701 ttcattcacc gaaagggcgc gcaacgctgg tacgtgaatg gctgcgtttg ttggcggatc
    32761 gcggcgttga cccactcaaa ctggaacaag aactgcctga atccggcgtc agtttgcgga
    32821 cgttaattgc ccgcacgcgc aatagttggc atgcgaataa aggcgaatat gacttctcac
    32881 acgaagtcaa agaggcgatg tcgggctgtc tggcctgtaa agcttgttcg actcagtgcc
    32941 ccatcaaaat tgatgtgccg gagtttcgct ctcgtttcct gcaactctat cacacccgtt
    33001 atttacgccc gctgcgcgac cacctcgtcg ctacggttga gagttacgca ccgctgatgg
    33061 cacgcgcgcc gaaaaccttt aacttcttca ttaaccagcc gctggtgcgc aaactctcgg
    33121 aaaaacatat cggcatggtt gatttgccgc tgctgtcggt cccctcgcta caacaacaaa
    33181 tggtggggca tcgctcggca aacatgacgc tggaacagct tgaagctctc aatgcagagc
    33241 agaaagcgcg cacggtgctg gtggtgcagg acccctttac cagctattac gatgctcaag
    33301 tggtggcgga ttttgtccgt ctggtcgaaa aattaggttt ccagcccgtg ttactgccat
    33361 tttcgccaaa tggcaaagcc cagcatatca aaggttttct taatcgtttt gcgaagacgg
    33421 cgaaaaagac ggcggatttc ctcaaccgta tggcgaagct gggtatgcca atggtaggcg
    33481 tcgacccggc gctggtactt tgttatcgcg atgaatataa actggctctg ggcgaggagc
    33541 gtggcgagtt taacgtctta ctggcgaatg aatggctggc aagcgcactt gagtcacagc
    33601 cagtggctac agtcagcggt gaatcatggt atttctttgg tcactgtacc gaagttaccg
    33661 ccttgccggg cgcgccagca caatgggcgg caatatttgc ccgttttggc gcgaaactgg
    33721 aaaatgtcag cgtgggttgc tgcggcatgg cagggactta cggacatgaa gcgaaaaatc
    33781 ataaaaattc gctcgggatc tatgaattat cctggcatca ggcgatgcag cgactgccgc
    33841 gtaaccgctg tctggcgacc ggatattcct gccgtagtca ggtaaaacgg gttgaaggca
    33901 cgggggtacg ccatcctgtg caggctttac tggagattat taaatgatat ggaaacgaaa
    33961 aatcaccctg gaagcactga atgctatggg ggaaggaaac atggtggggt tgctggatat
    34021 tcgctttgaa catattggtg atgacaccct tgaagcgaca atgccagtag actcgcggac
    34081 aaagcagcct ttcgggttgc tgcatggagg ggcatccgtg gtactggccg aaagtatcgg
    34141 ttccgttgcc agttatttat gtaccgaggg tgagcaaaaa gtggttggtc tggaaatcaa
    34201 tgctaaccac gtccgctcag cacgagaagg gcgggtgcgc ggcgtatgca aaccgttgca
    34261 tctcggttcg cgtcaccagg tctggcagat tgaaatcttc gatgagaaag ggcgtttgtg
    34321 ctgttcgtca cgattgacga ccgccatttt gtgacgttgt aaccagaaaa gtgtgacgca
    34381 tattgctttt ggttattatc tgcacagtaa acggtggtat actgtgcagg tcttgtctaa
    34441 aagcgaggat gctatgtcta ctcaacttga tcccacccaa ctggccattg aatttttacg
    34501 ccgtgaccaa agcaatcttt cacctgccca atatctcaaa cgtctgaaac aattagaact
    34561 tgaatttgct gacctgctta ctctctcttc tgcggaatta aaagaagaaa tctatttcgc
    34621 ctggcgtctg ggcgtgcatt gatcctgatt taatgtcgcg agttcgccaa atcggcctgg
    34681 attaatcttc gtccaggttc gagggccttc ttaatactgc ctatctaccc gccttataac
    34741 gttcaaaaat aatacaaatc gcagtttgtg ttaaaacagt gggttagctt tatgagatta
    34801 tccttatcgc cccttcaaga gctaagccac tgtgagtgcc ggagataagc gccggatggg
    34861 gtagaaaccc ttaagcctgt gtcgcacaga cttaagggtt ttcttatttc tgaacgctgc
    34921 acttttttac ctatccctta aatagtcatt ttcaatacaa ctttttcttt ttcatcacag
    34981 ccttatgctc cttagataat aatcattatc taacaatgag atacctaatt cttagcgtgc
    35041 ctgttaaccc acaaatcagg gtctatgctt attaaaagca ggtaaaaagc atgttttacg
    35101 ctaaagcccc tgcgtttgct gggttgaact gataatcatt atcactaaca tgctgttata
    35161 cgctgaaagc gatgaagtga ggtaaatcga tggacatgca ttcaggaacc tttaatccac
    35221 aggatttcgc ctggcaaggc ttaacgctga cacccgcagc ggcgatacac atccgcgaac
    35281 tggtggcaaa gcagcctggt atgatcggcg tgcgcttagg cgtgaagcaa acgggctgcg
    35341 cgggctttgg ctatgtgctc gacagtgtta gcgagccgga caaagacgat ctgctgtttg
    35401 aacacgacgg cgcgaagctg tttgtcccgc tgcaagcgat gccttttatt gatggcacgg
    35461 aagtcgattt cgttcgtgaa ggacttaatc agatattcaa atttcacaac cctaaagccc
    35521 agaatgaatg tggctgtggc gaaagctttg gggtataggc ggtactatgt ctcgtaatac
    35581 tgaagcaact gacgatgtca aaacctggac cggcggcccg ctgaattata aagaaggatt
    35641 cttcacccag ttagccaccg atgagctggc aaaggggata aacgaagagg tggtgcgcgc
    35701 aatttcggcg aagcgtaatg agccggagtg gatgctggag tttcgtctca acgcctatca
    35761 cgcatggctg gagatggaag aaccgcactg gttgaaagcg cactacgaca agctgaatta
    35821 tcaggattac agctactact cggcaccatc gtgcggtaat tgtgacgaca actgcgcgtc
    35881 tgaacccggc gcggtgcagc aaactggcgc gaacgccttt ttaagtaaag aggtggaggc
    35941 ggcgtttgag cagttgggcg ttcccgtgcg ggaaggcaaa gaggtggcgg tggatgccat
    36001 tttcgactct gtttcggttg ccactactta tcgcgaaaaa ctggcggagc agggaattat
    36061 tttttgttcc tttggcgagg cgatccacga tcacccggaa ctggtgcgta aatatctcgg
    36121 caccgtggtg ccggggaatg acaacttctt tgccgcactt aatgcggcgg tagcctctga
    36181 cggtacgttt atttatgtgc ctaaaggcgt gcgttgcccg atggaacttt ccacctattt
    36241 tcgcattaac gcggaaaaaa ccgggcagtt tgagcgcacg attctggtgg ccgacgaaga
    36301 cagctacgtt agctacattg aaggctgttc cgctccggtg cgtgacagct atcagttaca
    36361 cgcggcggtg gtggaagtca tcatccataa aaacgccgag gtgaaatatt ccacggtaca
    36421 aaactggttc cctggcgata acaacaccgg cggtattctc aacttcgtca ccaagcgtgc
    36481 tttgtgcgaa ggcgaaaaca gcaaaatgtc atggacgcaa tcagaaaccg ggtcagcgat
    36541 tacgtggaaa tatcccagct gcattttgcg cggcgataac tccattggtg agttttactc
    36601 agtggcgctg accagcggtc atcaacaagc ggataccggc accaagatga tccacatcgg
    36661 taaaaacacc aaatcgacca ttatctcgaa agggatctct gccggacata gtcagaacag
    36721 ttatcgcggc ttagtgaaaa tcatgccgac ggcaaccaat gcgcgcaatt tcactcagtg
    36781 cgactcaatg ctgattggcg ctaattgtgg ggcgcatacc ttcccgtatg tcgaatgtcg
    36841 caataacagc gctcagctgg agcacgaggc aacgacatca cgtattggtg aagatcaact
    36901 gttttactgc ctgcaacgcg ggatcagcga agaagatgcc atctcgatga ttgttaacgg
    36961 tttttgcaaa gacgtgttct cggaactgcc gctggaattt gccgttgaag cacaaaaact
    37021 cctcgccatc agtcttgaac acagcgtcgg ataaggaata aacatgttaa gtattaaaga
    37081 gttacacgtc agcgtggaag ataaagctat cctgcgtgga ttaagcctcg atgttcgtcc
    37141 cggcgaagtt cacgccatta tggggccaaa cggttcgggc aaaagtacct tatcggcaac
    37201 gcttgccggg cgagaagatt atgaagtgac gagcggcacg gttgagttta aaggcaaaga
    37261 tttgcttgcg ctgtcgccgg aagatcgtgc aggtgaaggc atctttatgg ccttccagta
    37321 tccggtggaa attcccggcg tcagtaacca gtttttcctg caaacggcac ttaatgcggt
    37381 gcgcagctat cgcggccagg aaacgctcga ccgctttgat tttcaggatt tgatggaaga
    37441 gaaaatcgct ctcctgaaga tgccggaaga tttattaacc cgttcggtaa acgttggttt
    37501 ttccggcggc gagaaaaagc gcaacgatat tttgcaaatg gcggtgctgg aaccggagtt
    37561 atgcattctt gatgagtcag actccgggct ggatattgac gcattaaaag tggttgccga
    37621 tggcgtgaac tcgctgcgtg acggcaagcg ttcattcatc attgttacgc actaccaacg
    37681 cattctcgac tacatcaagc ctgattacgt ccatgtgctg tatcagggac gaattgtgaa
    37741 atccggcgat ttcacgttgg tcaaacaact ggaggagcag ggttatggct ggcttaccga
    37801 acagcagtaa cgcactgcaa cagtggcatc atttgtttga agctgaaggg gcgaaacgct
    37861 ccccgcaagc acagcagcat ttacaacaat tgctgcgtac cggactgccg acacgtaaac
    37921 atgaaaactg gaaatatacg ccgctggacg ggctgaccaa tagccagttt gtcagcattg
    37981 cgggggagat atccccacag cagcgtgatg ccttagcgtt aacattagac gccgtgcggc
    38041 tggtgtttgt cgatggacgt tacgtgcccg cactgagcga tgcaactgaa ggcagcggat
    38101 atgaagtgag cattaacgac gaccgtcagg ggttacccga cgctattcag gcggaagtgt
    38161 ttctgcattt gacggaaagc ctggcacaaa gcgtgacgca tatcgccgta aagcgcggtc
    38221 aacggccggc aaagccattg ctgttaatgc atatcaccca gggcgtggca ggtgaagagg
    38281 tgaatactgc ccattaccgg catcatctgg atctggcgga aggtgccgaa gcaacggtga
    38341 tcgaacattt tgtcagcctt aatgatgctc gtcacttcac cggcgcacgg ttcactatca
    38401 acgtcgcagc gaacgcccat ttgcagcata tcaagctggc gtttgaaaac ccgctcagtc
    38461 accactttgc tcataacgat ttgttgctgg ctgacgatgc caccgcattt agccacagtt
    38521 tcctgctggg tggcgcagtg ttacgacaca acaccagtac gcaactcaat ggcgaaaaca
    38581 gcacggtgcg gatcaatagc ctggcgatgc cggtgaaaaa cgaggtgtgt gatactcgta
    38641 cctggctgga acacaataaa ggtttttgta acagccgaca gttgcacaaa actatcgtca
    38701 gcgacaaagg ccgtgcggta tttaacggtt tgatcaacgt cacgcagcac gccatcaaaa
    38761 cggatggtca gatgaccaat aataatctgc tgatgggcaa actggcggaa gtggatacta
    38821 aaccgcagct ggaaatctat gcagatgatg tgaaatgcag ccacggcgcg acggtggggc
    38881 gtattgatga cgaacagatg ttttatctgc gctcgcgcgg gatcaatcag caagatgccc
    38941 agcagatgat catttacgcc tttgctgccg aactgacgga agcactgcgt gatgaggggc
    39001 ttaaacagca ggtgctggcc cgaatcggtc aacgactgcc aggaggtgca agatgacttt
    39061 ttccgtcgac aaagtgcggg ccgactttcc ggtgctttct cgtgaggtaa acggtttgcc
    39121 gctggcttat ctcgacagtg ccgccagtgc gcagaaacca agccaggtga ttgacgccga
    39181 ggctgagttt tatcgtcatg gctacgcggc ggtgcatcgc ggtattcata ccttaagcgc
    39241 ccaggcgacc gagaaaatgg agaacgtgcg caagcgggca tcgctgttta ttaatgcccg
    39301 ttcggcggaa gagctggtgt tcgtccgcgg cacgacggaa gggatcaatc tggtcgccaa
    39361 tagctggggc aacagcaatg tgcgggcggg cgataacatc atcatcagtc agatggagca
    39421 ccacgctaac attgtcccct ggcagatgct ttgtgcacgc gttggcgcag agctgcgtgt
    39481 gatcccgctc aaccccgacg gtactctgca actggagacg ctgcctacgc tgtttgatga
    39541 gaaaactcgt ctgctggcaa ttactcatgt ctccaacgtg cttgggacag aaaatccgct
    39601 ggcggagatg atcacacttg cgcatcaaca tggcgcaaaa gtgctggtgg atggcgctca
    39661 ggcggtgatg catcatccgg tggatgttca ggcgctggat tgcgattttt acgtgttctc
    39721 cgggcataaa ctgtatggcc ctaccggaat tggcattctt tatgtcaaag aagccttgtt
    39781 gcaggagatg ccgccgtggg aagggggcgg ttctatgatc gccaccgtca gcctgagtga
    39841 aggcactacc tggaccaaag caccatggcg gtttgaagcc ggtacaccca ataccggagg
    39901 catcattggt cttggcgcgg cactggagta tgtttcggcg ctggggctta ataacatagc
    39961 cgagtatgaa cagaatctga tgcattacgc gctatcacag ttggaatctg taccggatct
    40021 cactctctat ggcccacaaa acaggcttgg cgttattgct tttaatcttg gtaaacacca
    40081 cgcctatgat gttggcagtt ttctcgataa ttacggcatt gctgtgcgta ccggacatca
    40141 ctgcgcaatg ccattaatgg cctattacaa cgtcctcgcg atgtgtcggg cgtcgctggc
    40201 catgtataac acccatgaag aagtggatcg tctggtgacc ggcctgcaac gtattcaccg
    40261 tctgctggga taacagggag gcactatggc tttattgccg gataaagaaa aattgctgcg
    40321 taatttttta cgctgcgcca actgggaaga gaaatatctc tacattattg agttgggtca
    40381 gcgtctgccg gaattacgtg acgaagacaa aagcccgcaa aatagcatcc agggctgcca
    40441 gagtcaggtg tggattgtga tgcgtcaaaa tgcgcaggga attattgaat tacagggcga
    40501 cagcgatgcg gccattgtaa aagggcttat tgcggtcgtc tttattcttt acgatcaaat
    40561 gacgccgcag gatattgtca atttcgatgt gcgcccgtgg tttgaaaaaa tggcgctcac
    40621 ccaacatctc accccatcac gctcacaagg tctggaagcg atgattcgcg caattcgcgc
    40681 caaagccgct gcacttagct aaactaaagg gacagctttc atccggttgg ctcacattgc
    40741 cgggtggtca atgtttacac tggcctgcac aggagtgttt tggtttcagg tgaacataag
    40801 cgccatccga cgtttcagcg tgagtctccg gcaaatacag gaggtttaca atgaaacgcg
    40861 cgtctttgct tacactcacg cttatcggcg cttttagcgc catccaggct gcctgggcgg
    40921 ttgattatca gctaccacca accggcagcc gactggttgg gcaaaatcaa acgtatacgg
    40981 tgcaagaagg ggataaaaac cttcaggcca tcgcccgacg ttttgatact gcggcaatgt
    41041 tgatccttga agccaataac actatcgccc cggtgccaaa acctggtacg acgataacta
    41101 ttccttcaca actgttatta cctgatgcgc cgcgtcaggg gattattgta aaccttgcag
    41161 agctgcgcct ttattattat ccgccgggag aaaatatcgt gcaggtttat ccgataggta
    41221 ttggtttgca ggggctggaa acgccggtga tggaaacgcg tgtggggcag aaaatcccta
    41281 acccaacctg gacgcctacg gcgggcattc gtcagcgttc gctggagcgt ggcattaaat
    41341 taccgccggt cgttcctgcc gggcccaata acccactagg acgttacgca ctgcgcctcg
    41401 cgcatggtaa tggcgaatac ctcattcatg gcaccagtgc gccggacagc gtcggtttgc
    41461 gcgtcagttc gggctgtatt cgcatgaatg caccggatat taaagcttta ttctccagcg
    41521 tgcggacggg agcgccggtg aaagtgatca acgaaccggt gaaatattcc gtggaaccta
    41581 acgggatgcg ttatgttgaa gtacatcgac cactatcggc agaagaacag cagaacgttc
    41641 agacaatgcc gtacacgctg ccagcaggct ttacgcaatt taaagacaat aaggctgtag
    41701 atcagaagtt agtcgataaa gcgttgtatc gtcgggcagg gtatccggtt gcggtgagca
    41761 gtggagcaac tcccgcagcc agcaatgcgc cttcagtaga gtcagcgcag aatggggaac
    41821 cagagcaagg gaatatgtta cgcgcgacgc agtagcggta aatagcaggc aaaaaaatgg
    41881 cgcacaatgt gcgccatttt tcacttcaca ggtactatta cttgcggtat ttagtagcca
    41941 tgttgtccag acgctggtta gcacgagctg cgtcatcttt agcagcctga acgtcggaac
    42001 gcattgcgtt cacgtcgttg ctcagctggt caactttagc gttcagagtc tgaacgtcag
    42061 aagacagctg atcgatttta gcgttgctgg agcaacctgc cagcagagta gaacccagga
    42121 ttaccgcgcc cagtaccagt ttagtagctt tcattattaa taccctctag attgagt
//
LOCUS       DAHHTG010000026        42054 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.026, whole genome shotgun sequence.
ACCESSION   DAHHTG010000026 DAHHTG010000000
VERSION     DAHHTG010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 42054)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42054)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42054
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.026"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            73..348
                     /locus_tag="NBI74_001405"
     CDS             73..348
                     /locus_tag="NBI74_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1931766.1"
                     /translation="MPTVLSRIAMQLKKIAWIIPVFMVSGCSLSPAIPVIGAYYPGWF
                     FCAIASLILTLITRRIIQRANINLAFVGIIYTALFALYAMLFWLAFF"
     gene            367..1398
                     /gene="mdtN"
                     /locus_tag="NBI74_001406"
     CDS             367..1398
                     /gene="mdtN"
                     /locus_tag="NBI74_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter periplasmic adaptor
                     subunit MdtN"
                     /protein_id="HCD1931767.1"
                     /translation="MESTPKKAPRSKFPALLVVALALVALVFVIWRVDSAPSTNDAYA
                     SADTIDVVPEVSGRIVELAVTDNQAVKQGDLLFRIDPRPYEANLAKAEASLAALDKQI
                     MLTQRSVDAQQFGADSVNATVEKARAAAKQASDTLRRTEPLLREGFVSAEEVDRARTA
                     QRAAEADLNAVLLQAQSAASAVSGVDALVAQRAAVEADIALTKLHLEMATVRAPFDGR
                     VISLKTSVGQFASAMRPIFTLIDTRHWYVIANFRETDLKNIRSGTPATIRLMSDSGKT
                     FEGKVDSIGYGVLPDDGGLVLGGLPKVSRSINWVRVAQRFPVKIMVDKPDPEMFRIGA
                     SAVANLEPQ"
     gene            1398..3449
                     /gene="mdtO"
                     /locus_tag="NBI74_001407"
     CDS             1398..3449
                     /gene="mdtO"
                     /locus_tag="NBI74_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313090.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter permease subunit
                     MdtO"
                     /protein_id="HCD1931768.1"
                     /translation="MSALNSLPLPVVRLLAFFHEELSERRPGRVPQTMQLWVGCLLVI
                     LISMTFEIPFVALSLAVLFYGIQSNAFYTKFVAILFVVATVLEIGSLFLIYKWSYGEP
                     LIRLIIAGPILMGCMFLMRTHRLGLVFFAVAIVAIYGQTFPAMLDYPEAVVRLTLWCI
                     VVGLYPTLLMTLIGVLWFPSRAITQMHQALNDRLDDAISHLTDSLAPLPETRIEREAL
                     ALQKLNVFCLADDANWRIQSAWWQSCVATVTYIYSTLNRYDPTSFADSQAIIEFRQKL
                     ASEINNLQHSITEGQCWQSDWRITESEAMAARECNLENICQTLLQLGQMDPNTPPTPA
                     TKPPSIVADAFTNPDYMRYAVKTLLACLICYTFYSGVDWEGIHTCMLTCVIVANPNVG
                     SSYQKMVLRFGGAFCGAILALLFTLLVMPWLDNIVELLFVLAPIFLLGAWIATSSERS
                     SYIGTQMVVTFALATLENVFGPVYDLVEIRDRALGIIIGTVVSAVIYTFVWPESEART
                     LPQKLAGALGMLSKVMRIPRQQEVTALRTYLQIRIGLHAAFNACEEMCQRVVLERQLD
                     SEERALLIERSQTVIRQGRDILHAWDATWNSAQALDNALQPDRAGQFADALEKYAAGL
                     ATALSRSPQITLEETPASQAILPTLLKQEQHVCQLFARLPDWTAPALTPAKEQAQGAT
                     Q"
     gene            3446..4912
                     /gene="mdtP"
                     /locus_tag="NBI74_001408"
     CDS             3446..4912
                     /gene="mdtP"
                     /locus_tag="NBI74_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter outer membrane
                     subunit MdtP"
                     /protein_id="HCD1931769.1"
                     /translation="MINRQLSRLLLCSILGSTTLISGCALVRKDSAPHQQLKPEQIKL
                     ADDIHLASSGWPQAQWWKQLNDPQLDALIQRTLSGSHTLAEAKLREEKAQSQADLLDA
                     GSQLQVAALGMLNRQRVSANGFLSPYAMDAPALGMDGPYYTEATVGLFAGLDLDLWGV
                     HRSAVAAAIGAHNAALAETAAVELSLTTGVAQLYYSMQASYQMLDLLEQTRDVIDYAV
                     KAHQSKVAHGLEAQVPFHGARAQILAVDKQIAAVKGQITETRESLRALIGAGASDMPE
                     IKPVALPQVQTGIPATLSYELLARRPDLQAMRWYVQASLDQVDSARALFYPSFDIKAF
                     FGLDSIHLDTLFKKTSRQFNFIPGLKLPLFDGGRLNANLEGTRAASNMMIERYNQSVL
                     NAVRDVAVNGTRLQTLNDEREMQAERVEATRFTQRAAEAAYQRGLTSRLQATEARLPV
                     LAEEMSLLMLDSRRVIQSIQLMKSLGGGYQAAPVVEKK"
     gene            5110..7257
                     /locus_tag="NBI74_001409"
     CDS             5110..7257
                     /locus_tag="NBI74_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:5527..5529,aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase H subunit alpha,
                     selenocysteine-containing"
                     /protein_id="HCD1931770.1"
                     /translation="MKKVVTVCPYCASGCKINLVVDNGKIVRAEAAQGKTNQGTLCLK
                     GYYGWDFINDTQILTPRLKTPMIRRQRGGKLEPVSWDEALNYVAERLSAIKEKYGPDA
                     IQTTGSSRGTGNETNYVMQKFARAVIGTNNVDCCARVUHGPSVAGLHQSVGNGAMSNA
                     INEIDNTDLVFVFGYNPADSHPIVANHVINAKRNGAKIIVCDPRKIETARIADMHIAL
                     KNGSNIALLNAMGHVIIEENLYDKAFVASRTEGFEEYRKIVEGYTPESVEDITGVSAS
                     EIRQAARMYAQAESAAILWGMGVTQFYQGVETVRSLTSLAMLTGNLGKPHAGVNPVRG
                     QNNVQGACDMGALPDTYPGYQYVKDPANREKFAKAWGVESLPAHTGYRISELPHRVAH
                     GEVRAAYIMGEDPLQTDAELSAVRKAFEDLELVIVQDIFMTKTASAADVILPSTSWGE
                     HEGVFTAADRGFQRFFKAVEPKWDLKTDWQIISEIATRMGYPMHYNNTQEIWDELRHL
                     CPDFYGATYEKMGELGFIQWPCRDTSDADQGTSYLFKEKFDTPNGLAQFFTCDWVAPI
                     DKLTDEYPMVLSTVREVGHYSCRSMTGNCAALAALADEPGYAQINTEDAKRLGIEDEA
                     LVWVHSRKGKIITRAQVSDRPNKGAIYMTYQWWIGACNELVTENLSPITKTPEYKYCA
                     VRVEPIADQRAAEQYVIDEYNKLKTRLREAALA"
     gene            7632..9200
                     /locus_tag="NBI74_001410"
     CDS             7632..9200
                     /locus_tag="NBI74_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCD1931771.1"
                     /translation="MIKKLLPLLVLSTISATTVAATPPNTLVVAQGLDDIVSLDPAEA
                     NELSSIQTVPSLYQRVVQPDRNDPTKITPILAESWQADPAAKTLTIKLKSDAKFASGN
                     PVRPEDVIYSYVRAVTLNKSGAFILNVLGWQPENIASQLKKIDDHTVQVQWSADVSPA
                     LALNILSTPIASIVDEKLVAPNAKNNDFGNEWLKMHSAGSGAFKMRTYQPHQAIVMEA
                     NASSPTGAPKLKSVIIKNVPDPATRRLLLQQGDADMARDLGADQIDALQGKPGVKVMS
                     IASAEQNYLAFNTGNKDNPLMSNPAFWEAARWLVDYDGITKNLLKGQYFTHQSFLPVG
                     FPGALEETPFTFNPAKAKEILAKAGIKDPHFTLDVENKPPFITIAQSIQASFAQGGVK
                     VDLLPAAGSQVYSRVRAHQHQGAIRMWLPDYFDAHSNASSFAYNDGKSSTVAGLNGWK
                     IPELNKETLAAIAEPDSAKRLDLYKKMQQELQRSSPYVFIDQGKTQIVMRDNVQGYQQ
                     GLNADMVWFDQVTK"
     gene            9300..10349
                     /locus_tag="NBI74_001411"
     CDS             9300..10349
                     /locus_tag="NBI74_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012579036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCD1931772.1"
                     /translation="MSVVFSTRARWRGKRPLLALLQGLFTVALTLLGLLLITFALSAL
                     SPVDRVLQIVGDHASQSTYDQVRHQLGLDQPLAVQFWHYLMNLAHGDLGIASSTGQPV
                     LKDLLSAFPATIELATLALIVGAVVGVIAGVLCARYVGSPLDFTVRTLTLLGNSVPVF
                     WLGLLMLALFYARLQWSAGPGRLDDIYQFTIEPRSGFALIDTWLSGDREAFTNAINHL
                     ILPVLLPAYFSLASITRLTRSACLGEMNKEYILLARAKGASEMTILLRHVLPNIRGTL
                     LTVIALAYTSMLEGAVLTETVFSWPGIGRYLTTALFAGDTTAIMGGTLLIGVCFVLIN
                     NLTDLLVRLTDPRLR"
     gene            10349..11182
                     /locus_tag="NBI74_001412"
     CDS             10349..11182
                     /locus_tag="NBI74_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCD1931773.1"
                     /translation="MPMFLFLRRLRHSPAAFCGLIIILLLMLIALFAPWLAPHDPNWQ
                     DAAARLQAPSRQHWLGTDSYGRDLLSRLIYGSRPALGLVALVTIITLPVGLLIGILAG
                     YYGGWLERVLMRFTDIVMSMPRLILAFAFVAMLGPGLVNGALALALTTWPAYARQARS
                     EIQRLRHSDYLAAAEMMGIRGRRLLAGHILPLCLPSAIVRLALDLAGIILAAAGLGFL
                     GLGARPPMAEWGAMIADGMQVIFDQWWIAAAPGAIILIASLAFNLLGDGLRDVLEPQH
                     D"
     gene            11175..12011
                     /locus_tag="NBI74_001413"
     CDS             11175..12011
                     /locus_tag="NBI74_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001517582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCD1931774.1"
                     /translation="MTDSRLSINNLCVDYPESRVVNNVSFTLGNERLALVGESGSGKS
                     MTARALMGLVRKPGVVTADTLNILGRDALTLNARGWQQLRGNDIAMVLQDPRYALNPV
                     KTVKAQLEEALTLHQRLNRREKEEKINTAIQAVGLNTRVLQSYPRELSGGMGQRVMIA
                     IALINDPRVLIADEPTSALDVRLRNQILELLVTQCEQRQMAMLLISHDLPLVAQFCHR
                     VLVMYQGNKVDEMHAAALPTATHPYTRTLWTCRPNAQTYGQMLPTLDRTAMTPEKYHD
                     DC"
     gene            11998..12702
                     /locus_tag="NBI74_001414"
     CDS             11998..12702
                     /locus_tag="NBI74_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCD1931775.1"
                     /translation="MTIVEINHLQVTFAEKTAVSAASFSVNAGETFSLIGESGCGKST
                     ILRVIAGLQRDWHGQVSLFGHTIKPGMRFQGDLRRNVQMVFQDPYASLHPNHTLWRTL
                     AEPLKIRGEREIEKRVQTALEQVGLPFDAARRYPHQLSGGQRQRVVIARALLLRPQLL
                     LLDEPTSALDMSVQAEILNLLNQLKLEHQMTYLLVSHDADVIAHMSDRAALMSEGKIQ
                     RFFDRDAMEKGEHRMD"
     gene            12813..13502
                     /locus_tag="NBI74_001415"
     CDS             12813..13502
                     /locus_tag="NBI74_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HCD1931776.1"
                     /translation="MKKIITLMLFLTFFAHANDSEPGSQYLKAAEAGDRRAQYFLADS
                     WFSSGDLSKAEYWAQKAADSGDADACALLAQIKITNPVSLDYPQAKVLAEKAAQAGSK
                     EGEVTLAHILVNTQAGKPDYPKAISLLENASEDLENDSAVDAQMLLGLIYANGVGIKA
                     DDDKATWYFKRSSAISRTGYSEYWAGMMFLNGEEGFIEKNKQKALHWLNLSCMEGFDT
                     GCEEFEKLTNG"
     gene            complement(13991..15304)
                     /gene="gltP"
                     /locus_tag="NBI74_001416"
     CDS             complement(13991..15304)
                     /gene="gltP"
                     /locus_tag="NBI74_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate:proton symporter GltP"
                     /protein_id="HCD1931777.1"
                     /translation="MKNIKFSLAWQILFAMVLGILLGSYLHYHSDSRDWLVVNLLSPA
                     GDIFIHLIKMIVVPIVISTLVVGIAGVGDAKQLGRIGAKTIIYFEVITTVAIILGITL
                     ANVFQPGAGVDMSQLATVDISKYQSTTEAVQSSSHGIMGTILSLVPTNIVASMAKGEM
                     LPIIFFSVLFGLGLSSLPATHREPLVTVFRSISETMFKVTHMVMRYAPVGVFALIAVT
                     VANFGFSSLWPLAKLVLLVHFAILFFALVVLGIVARLCGLSVWILIRILKDELILAYS
                     TASSESVLPRIIEKMEAYGAPASITSFVVPTGYSFNLDGSTLYQSIAAIFIAQLYGID
                     LSIWQEIILVLTLMVTSKGIAGVPGVSFVVLLATLGSVGIPLEGLAFIAGVDRILDMA
                     RTALNVVGNALAVLVIAKWEHKFDRKKALAYEREMLGKFDKTADQ"
     gene            complement(15647..16243)
                     /gene="nrfG"
                     /locus_tag="NBI74_001417"
     CDS             complement(15647..16243)
                     /gene="nrfG"
                     /locus_tag="NBI74_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfG"
                     /protein_id="HCD1931778.1"
                     /translation="MKQPQIPVKMLTTLTILMVFLCVGSYLLSPKWQAVRAEYQRQRD
                     PLHQFASQQTPEAQLQALQDKIRANPQNSEQWALLGEYYLWQNDYSNSLLAYRQALQL
                     RGENAELYAALATVLYYQASQHMTVQARAMIDKALALDSNEITALMLLASDAFMQANY
                     AQAIELWQKVMDLNSPRINRTQLVESINMAKLLQRRSD"
     gene            complement(16240..16623)
                     /gene="nrfF"
                     /locus_tag="NBI74_001418"
     CDS             complement(16240..16623)
                     /gene="nrfF"
                     /locus_tag="NBI74_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfF"
                     /protein_id="HCD1931779.1"
                     /translation="MNKGLLTLLLLFTCFARAQVVDTWQFANPQQQEQALNIASQLRC
                     PQCQNQNLLESNAPVAVSMRHQVYTMVEEGKSEVEIIGWMTERYGDFVRYNPPLTGQT
                     LVLWALPVVLLLLMALILWRVRAKR"
     gene            complement(16616..18334)
                     /locus_tag="NBI74_001419"
     CDS             complement(16616..18334)
                     /locus_tag="NBI74_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014640253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HCD1931780.1"
                     /translation="MDVFLPEVGFLALLLSLGVNVLIPLTAFAGVRLRWPAMMRLTCI
                     GILAQFALLLLAFGVLTYCFLISDFSVIYVAQHSYSLLSWELKLAAVWGGHEGSLLLW
                     VLLLSAWSALFAWHYRQQTDPLFPLTLAVLSLMLAALLLFVVLWSDPFVRIFPPAIEG
                     RDLNPMLQHPGLIFHPPLLYLGYGGLMVAASVALASLLRGEFDGTCARICWRWALPGW
                     SALTAGIILGSWWAYCELGWGGWWFWDPVENASLLPWLSATALLHSLSLTRQRGIFRH
                     WSLLLTIVTLMLSLLGTLIVRSGILVSVHAFALDNVRAVPLFGLFVLISLASLALYGW
                     RARDGGPAVRFSGLSREMLILATLLLFCAVLLIVLVGTLYPMIYGLLGWGRLSVGAPY
                     FNRATLPFALLMLVVIVLATFVSGKRARLPTLLAHAGVLLFAAGIVVSSVSRQEISLN
                     LQPGQQVTLAGYTFRFERLDLQAKGNYTSEKAIVALFDHQQRIGELTPERRFYEARRQ
                     QMMEPSIRWNGIHDWYAVMGEKTGADRYAFRLYVQSGVRWIWGGGLLMIAGALLSGWR
                     GRKRDE"
     gene            complement(18354..19310)
                     /gene="nrfD"
                     /locus_tag="NBI74_001420"
     CDS             complement(18354..19310)
                     /gene="nrfD"
                     /locus_tag="NBI74_001420"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase subunit NrfD"
                     /protein_id="HCD1931781.1"
                     /translation="MTQTSAFHFESLVWDWPIAIYLFLIGISAGLVTLAVLLRRFYPQ
                     AGGADSTLLRTTLIVGPGAVILGLLILVFHLTRPWTFWKLMFHYSFTSVMSMGVMLFQ
                     LYMVVLVLWLAKIFEHDLLALQQRWLPKLGIVQKVLSLLTPVHRGLETLMLVLAVLLG
                     AYTGFLLSALKSYPFLNNPILPVLFLFSGISSGAAVALIAMAIRQRSNPHSTEAHFVH
                     RMEIPVVWGEIFLLVAFFVGLALGDDGKVRALVAALGGGFWTWWFWLGVAGLGLIVPM
                     LLKPWVNRSSGIPAVLAACGASLVGVLMLRFFILYAGQLTVA"
     gene            complement(19307..19978)
                     /gene="nrfC"
                     /locus_tag="NBI74_001421"
     CDS             complement(19307..19978)
                     /gene="nrfC"
                     /locus_tag="NBI74_001421"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase Fe-S protein"
                     /protein_id="HCD1931782.1"
                     /translation="MTWSRRQFLTGVGVLAAVSGTAGRVVAKTLNINGVRYGMVHDES
                     LCIGCTACMDACRKVNKVPEGVSRLTIIRSEPQGEFPDVKYRFFRKSCQHCDHAPCVD
                     VCPTGASFRDAASGIVDVNPDLCVGCQYCIAACPYRVRFIHPVTKTADKCDFCRKTHL
                     QAGKLPACVEACPTKALTFGNLDDPNSEISQLLRQKPTYRYKLALGTKPKLYRVPFKY
                     GEVSQ"
     gene            complement(19975..20541)
                     /gene="nrfB"
                     /locus_tag="NBI74_001422"
     CDS             complement(19975..20541)
                     /gene="nrfB"
                     /locus_tag="NBI74_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418495.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase pentaheme
                     subunit"
                     /protein_id="HCD1931783.1"
                     /translation="MSVLRSLLTAGVLASGLLWSLNGITATPAAQASGDRYEVTQQRN
                     PDAACLDCHKPDTEGMHGKHASVINPNNKLPVTCTNCHGQPSPQHREGVKDVMRFNEP
                     MYKVGEQNSVCMSCHLPEQLQKAFWPHDVHVTKVACASCHSLHPQQDTMQTLSDKGRI
                     KICVDCHSDQRTNPNFNPASVPLLKEQP"
     gene            complement(20586..22022)
                     /gene="nrfA"
                     /locus_tag="NBI74_001423"
     CDS             complement(20586..22022)
                     /gene="nrfA"
                     /locus_tag="NBI74_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-forming nitrite reductase cytochrome
                     c552 subunit"
                     /protein_id="HCD1931784.1"
                     /translation="MTRIKINARRIFSLLIPFFFFTSVHAEQTAAPATPVTVEAKNET
                     FAPQHPDQYLSWKATSEQSERVDALAEDPRLVILWAGYPFSRDYNKPRGHAFAVTDVR
                     ETLRTGAPKNAEDGPLPMACWSCKSPDVARLIQKDGEDGYFHGKWARGGPEIVNNLGC
                     ADCHNTASPEFAKGKPELTLSRPYAARAMEAIGKPFEKAGRFDQQSMVCGQCHVEYYF
                     DGKNKAVKFPWDDGMKVENMEQYYDKIAFSDWTNSLSKTPMLKAQHPEYETWTAGIHG
                     KNNVTCIDCHMPKVQNAEGKLYTDHKIGNPFDNFAQTCANCHTQDKAALQKVVAERKQ
                     SINDLKIKVEDQLVHAHFEAKAALDAGATEAEMKPIQDDIRHAQWRWDLAIASHGIHM
                     HAPEEGLRMLGTAMDKAADARTKLARLLATKGITHEIQIPDISTKEKAQQAIGLNMEQ
                     IKAEKQDFIKTVIPQWEEQARKNGLLSQ"
     gene            22414..24372
                     /gene="acs"
                     /locus_tag="NBI74_001424"
     CDS             22414..24372
                     /gene="acs"
                     /locus_tag="NBI74_001424"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HCD1931785.1"
                     /translation="MSQIHKHTIPANIADRCLINPQQYEAMYQQSIDAPDTFWGEQGK
                     ILDWITPYQKVKNTSFAPGNVSIKWYEDGTLNLAANCLDRHLQENGDRTAIIWEGDDA
                     SQSKHISYKELHRDVCRFANTLLELGIKKGDVVAIYMPMVPEAAVAMLACARIGAVHS
                     VIFGGFSPEAVAGRIIDSSSRLVITSDEGVRAGRSIPLKKNVDDALKNPNVTSVEHVV
                     VLKRTGGKIDWQEGRDLWWHDLVEQASDQHQAEEMNAEDPLFILYTSGSTGKPKGVLH
                     TTGGYLVYAALTFKYVFDYHPGDIYWCTADVGWVTGHSYLLYGPLACGATTLMFEGVP
                     NWPTPARMAQVVDKHQVNILYTAPTAIRALMAEGDKAIEGTDRSSLRILGSVGEPINP
                     EAWEWYWKKIGNEKCPVVDTWWQTETGGFMITPLPGATELKAGSATRPFFGVQPALVD
                     NEGNPLKGATEGSLVITDSWPGQARTLFGDHERFEQTYFSTFKNMYFSGDGARRDEDG
                     YYWITGRVDDVLNVSGHRLGTAEIESALVAHPKIAEAAVVGIPHNIKGQAIYAYVTLN
                     HGEEPSPELYAEVRNWVRKEIGPLATPDVLHWTDSLPKTRSGKIMRRILRKIAAGDTS
                     NLGDTSTLADPGVVEKLLEEKQAIAMPS"
     gene            24582..24896
                     /locus_tag="NBI74_001425"
     CDS             24582..24896
                     /locus_tag="NBI74_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF485 domain-containing protein"
                     /protein_id="HCD1931786.1"
                     /translation="MNGTIYQRIEDNAHFRELVEKRQRFATILSIIMLAVYIGFILLI
                     AFAPGWLGTPLNPNTSVTRGIPVGVGVIVISFVLTGIYIWRANGEFDRLNNEVLHEVQ
                     AS"
     gene            24893..26542
                     /gene="actP"
                     /locus_tag="NBI74_001426"
     CDS             24893..26542
                     /gene="actP"
                     /locus_tag="NBI74_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation/acetate symporter ActP"
                     /protein_id="HCD1931787.1"
                     /translation="MKRVLTALAATLPFAANAADAISGAVERQPTNWQAIIMFLIFVV
                     FTLGITYWASKRVRSRNDYYTAGGNITGFQNGLAIAGDYMSAASFLGISALVFTSGYD
                     GLIYSLGFLVGWPIILFLIAERLRNLGRYTFADVASYRLKQGPIRILSACGSLVVVAL
                     YLIAQMVGAGKLIELLFGLNYHIAVVLVGVLMMMYVLFGGMLATTWVQIIKAVLLLFG
                     ASFMAFMVMKHVGFSFNNLFSEAMAVHPKGVDIMKPGGLVKDPISALSLGLGLMFGTA
                     GLPHILMRFFTVSDAREARKSVFYATGFMGYFYILTFIIGFGAIMLVGANPEYKDAAG
                     HLIGGNNMAAVHLANAVGGNLFLGFISAVAFATILAVVAGLTLAGASAVSHDLYANVF
                     KKGATEREELRVSKITVLILGVIAIILGVLFENQNIAFMVGLAFAIAASCNFPIILLS
                     MYWSKLTTRGAMLGGWLGLITAVVLMILGPTIWVQILGHEKAIFPYEYPALFSISVAF
                     LGIWFFSATDNSAEGARERELFRAQFIRSQTGFGVEQGRAH"
     gene            complement(26581..27270)
                     /locus_tag="NBI74_001427"
     CDS             complement(26581..27270)
                     /locus_tag="NBI74_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HCD1931788.1"
                     /translation="MANFQLSMLCLAITLVIYFLNKRLYRRFRKLPLMPLVLTPTLLV
                     MLLVFGNISWQNYIGEAHWLLWLLGPATIAFAVPVYDNRAIIKRHWMSLTAGVLTATV
                     VAVTSSVWLARLFTLPDEIQRSLAVRSVTTPFALAASQSLGGQPDLVALFVVVTGVFG
                     MAIGDVLFLRLSIREGMAKGAGFGAASHGAGTARSYELGQQEGVVASLVMMLSGVLMV
                     LVSPLVARMMF"
     gene            complement(27263..27673)
                     /locus_tag="NBI74_001428"
     CDS             complement(27263..27673)
                     /locus_tag="NBI74_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001143718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HCD1931789.1"
                     /translation="MPVALRRITPAVVQSIQVFFQVVLYAGLFIFAQYLVSWLHLPLP
                     ANLVGMILMLALIVCRILPLQWVRAGARWLLAEMLLFFVPAVVAVVNYAQLLLVDGWR
                     IFAVIALSTVMVLGTTAWVVEKVYRYEMRRLNRG"
     gene            27777..28661
                     /locus_tag="NBI74_001429"
     CDS             27777..28661
                     /locus_tag="NBI74_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016159883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCD1931790.1"
                     /translation="MDIRTLRYFVEVVRQQSFTRAAEKLFVTQPTISKMLKNLEDELN
                     CILLIRDGRRLLLTDTGRVVFERGLALLAEFRQLEAELSDINQLKTGVLRLGIPPMVG
                     MLMAGPIGLFRQRYPGVELKISEFGGLTVQQAVMNGELDMALTALPVEESGLTTLSLF
                     SHPLCVLTPHSGKWLECDAVSPETLAEHPLLIYNEDFALSKQLMKLFDEHGVKPRIAV
                     RSGQWDFLAAMVQAGVGIAILPEPICQRLDKNTLKWLPLESELSWKLGMIWREGVYLS
                     HSAHAWLSCCEGYWLTPE"
     gene            complement(28697..30346)
                     /locus_tag="NBI74_001430"
     CDS             complement(28697..30346)
                     /locus_tag="NBI74_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter"
                     /protein_id="HCD1931791.1"
                     /translation="MEIFFTILIMTLVVSLSGVVTRVMPFQIPLPLMQIAIGALLAWP
                     TFGLHVEFDPELFLVLFIPPLLFADGWKTPTREFLEHGREIFGLALALVVVTVVGIGF
                     LIYWVVPGIPLIPAFALAAVLSPTDAVALSGIVGEGRIPKKIMGILQGEALMNDASGL
                     VSLKFAVAVAMGTMIFTVGGATVEFMKVAIGGILAGFVVSWLYGRSLRFLSRWGGDEP
                     ATQIVLLFLLPFASYLIAEHIGVSGILAAVAAGMTITRSGVMRRAPLAMRLRANSTWA
                     MLEFVFNGMVFLLLGLQLPGILETSLMAAEIDPNVEIWMLFTDIILIYAALMLVRFGW
                     LWTMKKFSNRFLKKKPMEFGSWTTREILIASFAGVRGAITLAGVLSIPLLLPDGNVFP
                     ARYELVFLAAGVILFSLFVGVVMLPILLQHIEVADHSQQLKEERIARAATAEVAIVAI
                     QKMEERLAADTEENIDNQLLTEVSSRVIGNLRRRADGRNDVESSILEENLERRFRLAA
                     LRSERAELYHLRATREISNETLQKLLHDLDLLEALLIEENQ"
     gene            complement(30497..31846)
                     /gene="ghxP"
                     /locus_tag="NBI74_001431"
                     /gene_synonym="yjcD"
     CDS             complement(30497..31846)
                     /gene="ghxP"
                     /locus_tag="NBI74_001431"
                     /gene_synonym="yjcD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanine/hypoxanthine transporter GhxP"
                     /protein_id="HCD1931792.1"
                     /translation="MSTPSARTGGSLDAWFKISQRGSTVRQEVVAGLTTFLAMVYSVI
                     VVPGMLGKAGFPPAAVFVATCLVAGLGSIVMGLWANLPLAIGCAISLTAFTAFSLVLG
                     QHISVPVALGAVFLMGVLFTVISATGIRSWILRNLPHGVAHGTGIGIGLFLLLIAANG
                     VGLVIKNPLDGLPVALGDFTTFPVMMSLVGLAVIIGLEKLKVPGGILLTIIGISIVGL
                     IFDPNVHFSGVFAMPSLSDENGNSLIGSLDIMGALNPVVLPSVLALVMTAVFDATGTI
                     RAVAGQANLLDKDGQIIDGGKALTTDSMSSVFSGLVGAAPAAVYIESAAGTAAGGKTG
                     LTAITVGVLFLLILFLSPLSYLVPGYATAPALMYVGLLMLSNVAKIDFADFVDAMAGL
                     VTAVFIVLTCNIVTGIMIGFATLVIGRLVSGEWRKLNIGTVVIAVALVTFYAGGWAI"
     gene            complement(32393..32857)
                     /gene="soxR"
                     /locus_tag="NBI74_001432"
     CDS             complement(32393..32857)
                     /gene="soxR"
                     /locus_tag="NBI74_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensitive transcriptional activator SoxR"
                     /protein_id="HCD1931793.1"
                     /translation="MEKKLPRIKALLTPGEVAKRSGVAVSALHFYESKGLITSIRNSG
                     NQRRYKRDVLRYVAIIKIAQRIGIPLATIREAFGVLPEGHTLSAKEWKQLSSQWREEL
                     DRRIHTLVALRDELDGCIGCGCLSRSDCPLRNPGDRLGEEGTGARLLEDEQN"
     gene            32943..33266
                     /gene="soxS"
                     /locus_tag="NBI74_001433"
     CDS             32943..33266
                     /gene="soxS"
                     /locus_tag="NBI74_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide response transcriptional regulator
                     SoxS"
                     /protein_id="HCD1931794.1"
                     /translation="MSHQKIIQDLIAWIDEHIDQPLNIDVVAKKSGYSKWYLQRMFRT
                     VTHQTLGDYIRQRRLLLAAVELRTTERPIFDIAMDLGYVSQQTFSRVFRRQFDRTPSD
                     YRHRL"
     gene            complement(33269..34855)
                     /gene="pdeC"
                     /locus_tag="NBI74_001434"
     CDS             complement(33269..34855)
                     /gene="pdeC"
                     /locus_tag="NBI74_001434"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="c-di-GMP phosphodiesterase PdeC"
                     /protein_id="HCD1931795.1"
                     /translation="MSHRARHQLLAFPGIIFLVLFPIILSLWIAFFWAKSEVNNQLRT
                     FAQLALDKSELVIRQADLVSDAAERYQGQVCTPAHQKRMLNIIRGYLYINELIYARDN
                     HFLCSSLIASVNGYTIAPADYKREPNVSIYYYRDTPFFSGYKMTYMQRGNYVAVINPL
                     FWSEVMSDDPTLQWGVYDTVTKTFFSLSNEASAATFSPLIRLNDLTVQRNGYLYATVY
                     STKRPIAAIVATSYQRLITHFYNHLIFALPAGILGSLVLLLLWLRIRQNYLSPKRKLQ
                     RALEKHQLCLYYQPIIDIKTEKCIGAEALLRWPGEQGQVMNPAEFIPLAEKEGMIEQV
                     TDYVIDNVFRDLGAYLATHNDRYVSINLSASDFHTSRLIARTNQKTEQHAVRPQQIKF
                     EVTEHAFLDVDKMTPIILAFRKAGYEVAIDDFGIGYSNLHNLKSLNVDILKIDKSFVE
                     TLTTHKTSHLIAEHIIELAHSLGLKTIAEGVETEAQVNWLRKRGVRYCQGWFFAKAMP
                     PQVFMQWMEQLPARELTRGQ"
     gene            35284..35565
                     /locus_tag="NBI74_001435"
     CDS             35284..35565
                     /locus_tag="NBI74_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjcB family protein"
                     /protein_id="HCD1931796.1"
                     /translation="MATLTTGVVLLRWQLLSAVMMFLASTLNIRFRRADYVGLAVISS
                     GLGVVSACWFAMGLLGITMADITAIWHNIESVMIEEMNQTPPQWPMILT"
     gene            complement(35664..36200)
                     /gene="ssb1"
                     /locus_tag="NBI74_001436"
     CDS             complement(35664..36200)
                     /gene="ssb1"
                     /locus_tag="NBI74_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein SSB1"
                     /protein_id="HCD1931797.1"
                     /translation="MASRGVNKVILVGNLGQDPEVRYMPNGGAVANITLATSESWRDK
                     ATGEMKEQTEWHRVVLFGKLAEVASEYLRKGSQVYIEGQLRTRKWTDQSGQDRYTTEV
                     VVNVGGTMQMLGGRQGGGAPAGGNIGGGQPQGGWGQPQQPQGGNQFSGGAQSRPQQSA
                     PAAPSNEPPMDFDDDIPF"
     gene            36455..39277
                     /gene="uvrA"
                     /locus_tag="NBI74_001437"
     CDS             36455..39277
                     /gene="uvrA"
                     /locus_tag="NBI74_001437"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HCD1931798.1"
                     /translation="MDKIEVRGARTHNLKNINLVIPRDKLIVVTGLSGSGKSSLAFDT
                     LYAEGQRRYVESLSAYARQFLSLMEKPDVDHIEGLSPAISIEQKSTSHNPRSTVGTIT
                     EIHDYLRLLYARVGEPRCPDHDVPLAAQTVSQMVDNVLSQPEGKRLMLLAPIIKERKG
                     EHTKTLENLASQGYIRARIDGEVCDLSDPPKLELQKKHTIEVVVDRFKVRDDLTQRLA
                     ESFETALELSGGTAVVADMDDPKAEELLFSANFACPICGYSMRELEPRLFSFNNPAGA
                     CPTCDGLGVQQYFDPDRVIQNPELSLAGGAIRGWDRRNFYYFQMLKSLADHYKFDVEA
                     PWGSLSANVHKVVLYGSGKENIEFKYMNDRGDTSIRRHPFEGVLHNMERRYKETESSA
                     VREELAKFISNRPCASCEGTRLRREARHVYVENTPLPAISDMSIGHAMEFFNNLKLAG
                     QRAKIAEKILKEIGDRLKFLVNVGLNYLTLSRSAETLSGGEAQRIRLASQIGAGLVGV
                     MYVLDEPSIGLHQRDNERLLGTLIHLRDLGNTVIVVEHDEDAIRAADHVIDIGPGAGV
                     HGGEVVAEGPLEAIMAVPESLTGQYMSGKRKIEVPKKRVPANPEKVLKLTGARGNNLK
                     DVTLTLPVGLFTCITGVSGSGKSTLINDTLFPIAQRQLNGATIAEPAPYRDIQGLEHF
                     DKVIDIDQSPIGRTPRSNPATYTGVFTPVRELFAGVPESRARGYTPGRFSFNVRGGRC
                     EACQGDGVIKVEMHFLPDIYVPCDQCKGKRYNRETLEIKYKGKTIHEVLDMTIEEARE
                     FFDAVPALARKLQTLMDVGLTYIRLGQSATTLSGGEAQRVKLARELSKRGTGQTLYIL
                     DEPTTGLHFADIQQLLDVLHKLRDQGNTIVVIEHNLDVIKTADWIVDLGPEGGSGGGE
                     ILVSGTPETVAECEASHTARFLKPML"
     gene            complement(39312..39668)
                     /locus_tag="NBI74_001438"
     CDS             complement(39312..39668)
                     /locus_tag="NBI74_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HCD1931799.1"
                     /translation="MTISELLQYCMAKPGAEQSVHNDWKATQIKVEDVLFAMVKEVEN
                     RPAVSLKTSPELAELLRQQHSDVRPSRHLNKAHWSTVYLDGSLPDSQIYYLVDASYQQ
                     AVNLLPEEKRKLLVQL"
     gene            complement(39672..40088)
                     /locus_tag="NBI74_001439"
     CDS             complement(39672..40088)
                     /locus_tag="NBI74_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbQ family protein"
                     /protein_id="HCD1931800.1"
                     /translation="MWYQKTLTLSAKSRGFHLVTDEILNQLADMPRVNIGLLHLLLQH
                     TSASLTLNENCDPTVRHDMERFFLRAVPDNGNYEHDYEGADDMPSHIKSSMLGTSLVL
                     PVHKGRIQTGTWQGIWLGEHRIHGGSRRIIATLQGE"
     gene            complement(40199..40912)
                     /gene="aphA"
                     /locus_tag="NBI74_001440"
     CDS             complement(40199..40912)
                     /gene="aphA"
                     /locus_tag="NBI74_001440"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid phosphatase AphA"
                     /protein_id="HCD1931801.1"
                     /translation="MRKITQALSAVCLLFALNSSAVALASSPSPLNPGTNVAKLAEQA
                     PIHWVSVAQIENSLAGRPPMAVGFDIDDTVLFSSPGFWRGKKTFSPESEDYLKNPVFW
                     EKMNNGWDEFSIPKEVARQLIDMHVRRGDAIFFVTGRSPTKTETVSKTLADNFHIPAT
                     NMNPVIFAGDKPGQNTKSQWLQDKNIRIFYGDSDNDITAARDVGARGIRILRASNSTY
                     KPLPQAGAFGEEVIVNSEY"
     gene            41189..>42054
                     /locus_tag="NBI74_001441"
     CDS             41189..>42054
                     /locus_tag="NBI74_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000704417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HCD1931802.1"
                     /translation="MKISWNYIFKNKWRFHITSISLFLIMLAVSIAFLHLRFNTLSGT
                     DKMRLEMYKSTLYSTIEQFYVLPYMLSTDHIIRQAVITPDDMTSSELNQRIAHFNTQL
                     KTAAIFILDTQGKAIASSNWQDPGSYVGQNYSYRPYYKHAMSGLNGRFYGIGSTTNTP
                     GFFLSTSIKDKGKIVGVVVVKISLNEIEKAWAEGPENIIVNDEHGIIFLSSKSPWRMR
                     TLQPLPVQAKQKLQSTRQYSLDNLLPADYYPCYTVSNFTFLKDKKEQLCLFPQHYTQQ
                     IAIPEFNWKMTI"
BASE COUNT        10376 a        11267 c        10738 g         9673 t
ORIGIN      
        1 ccatactgac gccttcaatg gatattgata ttcactacag atattgttcg cagcagacag
       61 gatgtattta atatgccaac agttctctct cgcatagcga tgcaactcaa aaaaatagcc
      121 tggataattc ccgtcttcat ggtttcggga tgctcattat ctccggcaat cccggtgatc
      181 ggcgcttatt atcccggctg gtttttctgc gccattgcca gccttatttt gacgctcatc
      241 acgaggcgaa ttattcagcg ggcaaatatc aatctggcat ttgtcggaat tatttatacc
      301 gccctttttg ctctctacgc catgctgttc tggctggcat ttttctaatc aatttgagac
      361 gcgctcatgg aaagtacgcc gaaaaaagct cctcgcagta aattccctgc tctgttagtg
      421 gttgcgttgg cgctggttgc ccttgttttc gttatctggc gcgtagacag tgcgccatca
      481 actaatgacg cttacgcgtc agcagatacc attgatgtgg tgccggaagt cagcggtcgc
      541 attgtagaac tggcggtcac cgacaaccag gcggtgaaac agggcgattt gctgttccgc
      601 attgatccgc gcccgtacga agccaatctg gcgaaagctg aagcctccct cgcggcgctg
      661 gataagcaaa ttatgctcac ccagcgtagc gttgacgcac aacagtttgg tgccgattcg
      721 gttaatgcca cggtagaaaa agcccgtgcc gccgcgaaac aagccagcga tacgttacgc
      781 cgtaccgaac cgctactgcg agaaggtttt gtttctgcgg aagaggttga ccgtgcaaga
      841 acggcgcagc gcgcggcaga agccgatctt aatgccgtat tgttgcaggc gcagtcagcc
      901 gccagcgccg tcagcggcgt ggatgcatta gttgcccaac gtgcggctgt cgaagcggat
      961 atcgcgctga ccaaactgca tctggaaatg gcaaccgttc gcgcgccgtt tgatggccgg
     1021 gtcatttccc tcaaaacctc cgtcgggcaa tttgcttctg ccatgcgccc tattttcacc
     1081 ctaatcgaca ctcgtcactg gtacgtgatc gccaatttcc gcgaaactga tctgaaaaat
     1141 attcgctcag gtacacccgc aacgattcgc ctgatgagcg acagcggcaa aaccttcgag
     1201 ggtaaagtgg attcgattgg ctacggcgtg ctaccggatg acggcggcct ggtgctgggc
     1261 ggcctgccga aagtgtcacg gtctattaac tgggtccgcg ttgcccagcg ttttccggtc
     1321 aaaatcatgg ttgataaacc tgacccggaa atgttccgca tcggcgcttc ggcagtcgct
     1381 aatcttgagc cacaataatg agcgcgctca actccctgcc attaccggtg gtcaggctgc
     1441 tggcgttctt tcatgaagag ttaagcgagc ggcgaccagg gcgcgtgcca cagactatgc
     1501 aactctgggt aggctgcctg ctggtgattc tgatctcgat gacttttgag atcccttttg
     1561 tggcgttatc actggcagtg ctgttttacg gtattcagtc gaacgcgttt tacaccaaat
     1621 ttgtcgcgat cttgtttgtg gttgccacgg tgctggagat cggcagcctg tttttgatct
     1681 acaaatggtc atatggcgaa ccgttgatcc gattgatcat cgccgggccg atcctgatgg
     1741 gctgcatgtt tttgatgcgc acccatcggc tgggactggt ctttttcgcc gtcgccattg
     1801 tcgctattta cgggcaaacc ttccccgcta tgctcgacta tccggaagcg gtcgtgcgct
     1861 taacgctgtg gtgtatcgtt gttggcctct atccaacctt gctgatgacg ttaatcggcg
     1921 tgctgtggtt tcccagtcgt gccattacgc agatgcatca ggcgcttaat gatcggcttg
     1981 atgatgccat tagccacctg acggacagcc tcgcaccgct acccgaaacg cggattgaaa
     2041 gagaggcgct ggcgctgcaa aaactcaatg tcttttgcct cgcggacgat gccaactggc
     2101 gaatccagag cgcatggtgg caaagctgcg tggcaacggt aacctacatt tactcgacgc
     2161 tgaatcgcta cgatcccacc tcttttgctg attctcaggc aattattgaa ttccgacaaa
     2221 aattagcttc agaaatcaac aatctgcagc attccattac cgaaggtcag tgctggcaaa
     2281 gcgactggcg gatcactgaa agtgaagcga tggcggcacg ggaatgtaac ctggagaata
     2341 tctgccagac attgttgcaa ctgggtcaga tggacccgaa tacgccgcca acgcccgcca
     2401 ccaaaccgcc atcaatagtc gccgacgctt ttaccaatcc agactatatg cgctacgcgg
     2461 taaaaacgct gctcgcctgt ttgatctgtt acaccttcta cagcggcgtg gactgggaag
     2521 gcattcacac ctgtatgctg acctgcgtga tcgtcgctaa tccaaatgtc ggttcgtcgt
     2581 accagaagat ggtgctgcgt tttggtgggg ccttttgcgg cgcgattctg gcgctgttat
     2641 tcacgctact ggtcatgccc tggctggaca atattgtcga attgctgttt gtgctggcac
     2701 cgattttcct gttgggcgca tggattgcca ccagctctga acgctcttct tatatcggca
     2761 cacagatggt ggtcaccttc gcgctcgcca cgctcgaaaa cgtttttggc ccggtgtacg
     2821 acctggtgga aattcgcgat cgcgctctgg gtatcatcat tggtaccgtg gtgtcggcgg
     2881 tgatttacac ctttgtctgg cctgaaagcg aagcgcgcac gctgccgcaa aaactggctg
     2941 gcgcgctggg tatgttgagt aaagtaatgc ggatcccacg ccagcaggaa gtcacggctc
     3001 tgcgcactta tctgcaaatt cgcataggtc tgcatgcggc gtttaatgcc tgtgaagaga
     3061 tgtgccaacg cgtggtgctg gagcgtcaac tggacagcga agaacgcgcc ttactgattg
     3121 aacgttcgca aacggttatt cgtcagggcc gcgatattct tcacgcctgg gatgccacct
     3181 ggaactcggc gcaggcgctg gataacgcac tacagccgga cagagcaggt cagtttgccg
     3241 acgcactgga gaaatacgcc gctggtctgg caaccgcact cagccgttct cctcaaataa
     3301 cgcttgaaga aacacccgcc tcgcaggcca tcctgcccac cttattaaaa caggagcaac
     3361 acgtctgcca gcttttcgcc cgcttgccag actggacagc cccggcatta acgcccgcca
     3421 aggaacaggc acaaggagcc acacaatgat caatcgtcaa ctttcacgtc tgctgttgtg
     3481 cagcattctc ggcagcacga cgctgatttc cggctgtgcc ctggtacgta aggattctgc
     3541 gcctcatcaa cagctcaaac cggaacaaat caaactggcc gatgatattc atcttgccag
     3601 ctccggctgg ccgcaggcgc agtggtggaa acaactcaat gacccgcagc tggatgcgct
     3661 gatccaacgg acgctaagtg gttcacacac cctcgccgaa gcgaaactgc gggaagaaaa
     3721 agcgcagtcc caggccgatt tgttagatgc cggttcacaa ttacaggtcg cagcgttagg
     3781 gatgctcaac cgccaacgcg tctcggcgaa cggcttttta agcccttatg cgatggatgc
     3841 gccagcactg ggtatggacg ggccgtacta tacggaagcc acagtcggtt tgtttgccgg
     3901 actggatctc gatttgtggg gtgtgcatcg ctcagcggtt gctgccgcca ttggcgcgca
     3961 taatgccgcg ctggcagaaa ccgcagcagt agagctatcg ctgaccacgg gcgtagcgca
     4021 gctttattac agtatgcagg ccagctatca gatgctcgat ctgttagaac aaactcgcga
     4081 tgtgattgat tacgcggtga aagcgcacca gagtaaagtg gcgcacggtc tggaagcgca
     4141 agtgcctttc cacggcgcgc gggcacagat tctggcggtc gataaacaaa ttgctgccgt
     4201 caaagggcaa atcaccgaaa cgcgagaatc tctgcgtgca ttgattggcg cgggagccag
     4261 cgatatgccg gagatcaaac cggtggcatt accgcaagtc cagaccggta ttccggcgac
     4321 actctcttat gagttgctcg ccagacgccc ggatctgcaa gccatgcgct ggtatgttca
     4381 ggcgtcatta gatcaggtgg attccgcgcg ggcgctgttc tacccgagct ttgatatcaa
     4441 agcgtttttc ggtctggact ccatccacct ggatacctta ttcaaaaaaa ccagtcgcca
     4501 gttcaacttc atcccgggtc tgaaattgcc gctgtttgac ggtggacggt tgaatgccaa
     4561 tctcgaaggc actcgcgccg ccagcaacat gatgattgaa cgttacaacc agtcagtact
     4621 gaacgcggtg cgtgacgttg ccgtcaacgg cacacgtctg caaacgctca acgacgagcg
     4681 agagatgcag gctgaacgcg tagaagcaac gcgcttcacc cagcgcgcag ccgaggccgc
     4741 ttatcagcgc ggcttaacca gccgcttaca ggccaccgaa gcccggttgc cagtgcttgc
     4801 cgaagagatg tcattactga tgctggacag ccgccgggtg atccaaagta ttcagttgat
     4861 gaaatcgctg ggcggcgggt atcaggcggc tcccgtcgtc gagaaaaaat aaaatgtcta
     4921 ccgcgtgatg gctgtcacgc ggtatttcgt ttcgtcacgt caaaactgac gacagcctgt
     4981 ttttcgtcag agttttgaat aaatagtgcc cgtaatatca gggaatgacc ccaaataaaa
     5041 tgtggcataa aagatgcata ctgtagtcga gagcgcgtat gcgtgatttg attaactgga
     5101 gcgagaccga tgaaaaaagt cgtcacggtt tgcccctatt gcgcatcagg ttgcaaaatc
     5161 aacctggtcg tcgataacgg caaaatcgtc cgggcggagg cagcgcaggg gaaaaccaac
     5221 cagggtaccc tgtgtctgaa gggttattat ggctgggact tcattaacga tacccagatc
     5281 ctgaccccgc gcctgaaaac ccccatgatc cgtcgccagc gtggcggcaa actcgaacct
     5341 gtttcctggg atgaggcact gaattacgtt gccgagcgcc tgagcgccat caaagagaag
     5401 tacggtccgg atgccatcca gacgaccggc tcctcgcgtg gtacgggtaa cgaaaccaac
     5461 tatgtaatgc aaaaatttgc gcgcgccgtt attggtacca ataacgttga ctgctgcgct
     5521 cgtgtctgac acggcccatc ggttgcaggt ctgcaccaat cggtcggtaa tggcgcaatg
     5581 agcaatgcta ttaacgaaat tgataatacc gatttagtgt tcgttttcgg gtataacccg
     5641 gcggattccc acccaatcgt ggcgaatcac gtgattaacg ctaaacgtaa cggggcgaaa
     5701 attatcgtct gcgatccgcg caaaattgaa accgcgcgca ttgctgacat gcacattgca
     5761 ttgaaaaacg gctcgaacat cgcgctgttg aatgcgatgg gccatgtcat tattgaagaa
     5821 aatctgtacg acaaagcgtt cgtcgcttcc cgtacagaag gctttgaaga gtatcgtaaa
     5881 atcgttgaag gctacacgcc ggagtcggtt gaagatatca ccggcgtcag cgccagtgag
     5941 attcgtcagg cggcgcggat gtatgcccag gcagaaagcg ccgccatcct gtggggcatg
     6001 ggggtaaccc agttctatca gggcgtggaa accgtgcgtt ctctgaccag cctcgcgatg
     6061 ctgaccggta acctcggtaa gccacatgcg ggtgtaaacc cggttcgtgg tcagaacaac
     6121 gtgcagggtg cctgcgatat gggcgcgctg ccggatacgt atccgggcta tcagtacgtg
     6181 aaagatccgg ctaaccgcga gaaatttgcc aaagcctggg gcgtagagag cctgccagcg
     6241 cataccggtt atcgtatcag cgagctgccg caccgcgtgg cgcatggtga agtgagagcg
     6301 gcgtacatta tgggcgaaga tccgctgcaa actgacgcgg aactgtcggc agtacgtaaa
     6361 gcttttgaag atctggaact ggttatcgtt caggacatct ttatgaccaa aaccgcgtcg
     6421 gcggcggatg tcattttacc gtcaacgtcg tggggcgagc atgaaggcgt gtttactgcg
     6481 gctgaccgtg gcttccagcg tttcttcaag gcggttgaac cgaagtggga tctgaaaacg
     6541 gactggcaaa tcatcagtga aattgccact cgtatgggtt atccgatgca ctacaacaac
     6601 acccaggaga tctgggatga gttgcgtcat ctgtgcccgg atttctacgg tgcgacttac
     6661 gagaaaatgg gcgaactggg cttcattcag tggccttgcc gcgatacttc agatgccgat
     6721 caggggactt cttatctgtt taaagagaag tttgataccc cgaacggtct ggcgcagttc
     6781 ttcacctgcg actgggtagc gccaatcgac aaactcaccg acgagtaccc gatggtactg
     6841 tcaacggtgc gtgaagttgg tcactactct tgccgttcga tgaccggtaa ctgtgcggca
     6901 ctggcggcgc tggctgatga acctggctac gcacaaatca ataccgaaga cgccaaacgt
     6961 ctgggtattg aagatgaggc attggtttgg gtgcactcgc gtaaaggcaa aattatcacc
     7021 cgtgcgcagg tcagcgatcg tccgaacaaa ggggcgattt acatgaccta ccagtggtgg
     7081 attggtgcct gtaacgagct ggtgaccgaa aacttaagcc cgattacgaa aacgccggaa
     7141 tacaaatact gcgccgtgcg cgtcgagccg atcgccgatc agcgcgccgc cgagcagtac
     7201 gtgattgacg agtacaacaa gttgaaaact cgcctgcgcg aagcggcact ggcgtaatac
     7261 cgcgtaaact cacgttaaaa gcggcgatat tacccaacgc ctttgtatca atccggaaac
     7321 gactctctgt ttccggattt ttttatacat acctgcgcag tcaaaccagc ccatcctggt
     7381 tattagcgac gtcttctcgg ttgttcttct attccagtca cgattgcaaa tgagaattat
     7441 ctttactcaa ttctcatcgc ggcctaacgt aataatactt tacatttgat gaaccaaatt
     7501 tcggtaaggt gagaaatcat cgaccgttta actcgttcta ttaacgggat attttggcgc
     7561 gattaatgca acaaaactcc gtctgatagc atattaataa cctcaaagta atttcaggga
     7621 ttgggaagca tatgataaaa aaactattac ctttactggt tttaagcaca atttccgcca
     7681 caaccgttgc cgctacaccg cctaatacgt tggttgtagc ccaggggctg gatgatattg
     7741 tgagcctgga cccggcagaa gctaatgaac tctcaagtat tcagactgta ccaagtctgt
     7801 atcaacgcgt agtacaaccg gaccgtaatg atccgacgaa aatcactccg attctggcgg
     7861 aaagttggca ggctgacccc gcagccaaaa cgctaaccat aaaactgaaa agcgatgcga
     7921 aatttgcctc tggtaatccg gtacgcccgg aagatgtgat ttactcttat gtccgggctg
     7981 tcacactgaa taaatccggt gcgtttattc tcaatgtgct gggatggcag ccggaaaata
     8041 tcgccagcca gttgaagaaa atcgatgacc ataccgtcca ggtgcaatgg agtgccgacg
     8101 tcagcccggc gctggcactg aatattctct ccacaccaat cgcctcaatt gttgacgaga
     8161 agctggttgc gccaaacgcc aaaaataacg actttggtaa tgaatggctg aagatgcatt
     8221 ccgctggcag cggcgcgttt aaaatgcgta cttaccaacc gcatcaggcc atcgtgatgg
     8281 aagccaacgc cagctcacca accggcgccc cgaaactgaa aagcgtcatc atcaaaaatg
     8341 ttcccgatcc ggcaacacgt cgcctgctgc tccaacaagg tgatgcggat atggctcgtg
     8401 atctgggagc cgatcaaatc gatgcattac agggtaaacc tggcgtaaaa gtgatgagca
     8461 ttgcctccgc cgaacagaac tacctggcct tcaataccgg taacaaggat aacccgctga
     8521 tgagtaatcc tgcgttctgg gaggccgctc gctggttggt cgattacgac ggcatcacca
     8581 aaaatctctt aaaaggacaa tattttaccc atcagagttt cctgcccgtg ggctttcctg
     8641 gagcactgga agaaacaccg tttaccttta atccagccaa agcaaaagag atcctcgcta
     8701 aagccggaat caaagacccg cacttcacgc ttgatgtcga aaataaaccg ccatttatta
     8761 ccatcgcgca gtcaattcag gccagctttg ctcagggcgg tgtaaaagtt gatttgctgc
     8821 cagccgcagg gagtcaggtc tactctcgcg ttcgcgccca ccagcatcag ggggccattc
     8881 gtatgtggtt gcctgattat tttgacgcac attccaacgc cagctctttc gcctataacg
     8941 atggcaaatc cagcaccgtt gccgggttaa acggctggaa aatcccggaa ctgaacaaag
     9001 aaacgctggc ggccatcgct gaacctgata gcgctaaacg tcttgatttg tataagaaaa
     9061 tgcaacaaga actacaacgt agttccccgt atgtctttat tgatcaaggc aaaacgcaaa
     9121 ttgtcatgcg tgataatgtg caggggtatc aacaagggtt gaatgccgat atggtctggt
     9181 ttgatcaggt aaccaaataa ccctgtatgc aactcgcgtc cagccatgtt accgggcgcg
     9241 gtaccagggc tggcctgtat tgcaggccag ccgctgattt atcactgtat ggaaagatca
     9301 tgtctgtcgt tttttccact cgcgcgagat ggcgaggcaa acgccctcta ctcgcgctgt
     9361 tgcaagggct gtttaccgtt gctctgacgc tactcggcct gctgctgatt acctttgcgc
     9421 tttcggctct ctcaccggtc gatcgcgtac tacaaatcgt tggcgatcac gccagccagt
     9481 ccacctatga tcaggtacgc caccagttag gattggatca accgttagcc gtccagttct
     9541 ggcattacct gatgaatctg gcacacggcg atctgggcat cgccagctcc accgggcaac
     9601 cggtactcaa ggatttgcta tcagcattcc cggcgaccat tgaactggca acgttggcgc
     9661 ttattgtcgg tgctgttgtc ggcgtcattg cgggtgtact ttgcgcccgt tatgtcggtt
     9721 ctccgctgga tttcaccgtg cgaacattaa ctctactggg aaattccgtc cctgtttttt
     9781 ggcttggtct gctgatgctg gcactgttct acgccagact gcaatggagt gccgggccgg
     9841 gcaggctgga tgatatttat caattcacca tagaaccccg cagcggattt gcgctgattg
     9901 atacgtggct ttctggcgat cgcgaggcat ttaccaatgc aatcaaccat ttgatacttc
     9961 cggttttatt accggcgtac ttctcgctcg ccagtattac gcgcctgact cgttccgcct
    10021 gtcttggcga aatgaataaa gaatatattc tgctggcgcg cgccaaaggg gccagcgaga
    10081 tgacaatttt gctacgtcat gttttaccca acattcgcgg caccttgctc accgttatcg
    10141 ccctcgccta tacatcaatg ctggaaggcg cggtactgac agaaaccgtc ttttcatggc
    10201 caggtattgg gcgctacctc actacagcgc tcttcgcggg tgataccaca gcaatcatgg
    10261 gaggaacgct gctgattggc gtctgctttg tgttgataaa taacttaacc gacctgctgg
    10321 tccggctgac cgatccgagg ttacgctgat gccgatgttt ctctttttac gccgtttacg
    10381 ccattcaccc gccgcgtttt gcgggttgat aattatcctg ttattgatgc taattgcgct
    10441 cttcgcgccg tggctggcac ctcacgatcc taactggcag gatgcggcgg cgcgattaca
    10501 agcaccgagt cgtcagcact ggctcggcac tgacagttac gggcgagacc tactttcacg
    10561 tttaatctac ggcagccgcc cggcactggg gctggtggca ttggtgacaa tcattacact
    10621 cccggtcggt ctgctgattg gcattttagc cgggtactac ggcggctggc tggaacgcgt
    10681 gctgatgcgt tttactgaca tagtaatgtc aatgccgcgt ttaattctgg cttttgcttt
    10741 tgttgccatg ctggggccgg ggctggttaa cggcgcactg gcccttgcat taaccacctg
    10801 gcctgcttat gcgcgtcagg cacggagtga gatccaacgt ctgcgacaca gtgattatct
    10861 tgccgccgca gaaatgatgg gcatccgcgg caggcgtctg ctggctggtc atattttgcc
    10921 attgtgcctg ccatctgcca ttgtccgtct ggcgctggat ttagcgggta tcattctggc
    10981 ggctgctggt ctgggttttc ttgggcttgg tgctcgcccg ccaatggcgg aatggggagc
    11041 catgatcgcc gacggtatgc aggtcatttt tgatcaatgg tggattgccg ccgctcccgg
    11101 cgcaattatc ttaattgcca gtctggcctt taatctgctg ggtgatggct tgcgcgatgt
    11161 tctggagcct caacatgact gactcacgtc ttagtatcaa caatctctgc gtcgattatc
    11221 cggagtcccg ggtcgtcaac aatgtcagtt ttacattagg caatgaacgt ctggcactgg
    11281 tcggtgagtc gggttcaggt aagtccatga ccgcccgcgc cctgatgggg ctggtgcgta
    11341 aaccgggtgt agtaaccgcg gacacactta acattctggg gcgtgatgca ctcaccctta
    11401 acgcgcgtgg atggcaacag ttgcgcggta acgatatcgc gatggtattg caagatccac
    11461 gttacgcgct taatccagta aagacagtaa aagcgcaact ggaagaggcg cttacgctcc
    11521 atcaacgtct gaaccgccgc gagaaagaag agaaaataaa caccgcaatt caggctgtcg
    11581 gcctcaatac gcgagtactg caaagctatc ctcgtgaact ttctggcggc atggggcagc
    11641 gagtgatgat tgcaattgcg ctcatcaacg atccccgggt attgattgcc gatgagccaa
    11701 catcagcact ggatgtgcgc ctgcgtaatc agattctgga actgctggtt acccagtgtg
    11761 aacaacgtca gatggcaatg ttgctgatca gtcacgacct gccattagtt gcccaatttt
    11821 gccatcgggt gctggtgatg tatcagggaa ataaggtgga tgaaatgcac gctgctgcgc
    11881 tacctacagc aacgcatcct tacacgcgca cactgtggac ttgccgccca aatgcacaaa
    11941 cttacgggca gatgctgccg acgctggaca ggacggcaat gacgccggag aaataccatg
    12001 acgattgttg aaattaatca cttacaggtg acttttgctg agaaaaccgc agtttctgcc
    12061 gccagttttt cggttaatgc cggagagaca tttagtttaa ttggcgaatc aggctgcgga
    12121 aaatccacga ttttgcgcgt aattgctgga ttacagcgcg actggcacgg tcaggtgtca
    12181 ctgtttgggc acaccatcaa accaggaatg cgttttcagg gcgatctccg ccgcaatgtg
    12241 caaatggtgt ttcaggatcc ctatgcctcg ttgcatccca atcatactct gtggcgaacg
    12301 ctggctgaac cactcaaaat tcgtggcgaa agagaaatag aaaaacgcgt ccagactgca
    12361 ctggaacagg ttggcttgcc tttcgacgcc gctcgtcgct acccacacca gctttccggt
    12421 ggtcaacggc aacgtgtggt tattgctcgc gccctgttgt tacgaccaca acttcttctg
    12481 ctggatgaac ccacctcggc gttggatatg tcggtacaag cggaaatcct taatttgttg
    12541 aatcaactta agctggaaca ccaaatgaca tatctgctgg tcagtcacga tgccgatgtc
    12601 atcgcgcata tgtctgatcg agcggcattg atgtccgagg gaaaaattca gcgatttttt
    12661 gatcgcgacg ccatggaaaa gggggaacat cggatggatt aaaaaacctg acattgcatt
    12721 aattcattct gctttcaggt ctttttacag ttgttttttt gtccacccga acactttata
    12781 ctgtctctcc cctaccaaac ttcattatca aaatgaaaaa aattatcact ttgatgttgt
    12841 ttttgacatt ctttgcccac gcgaacgact ccgagcctgg cagccagtat ttaaaggcag
    12901 cagaggcagg ggaccgacgc gcacaatatt ttcttgccga cagctggttt agctccggcg
    12961 atttgagcaa agccgaatat tgggcacaga aagccgccga cagcggtgat gccgatgcct
    13021 gcgcgctgtt ggcgcagatc aaaatcacca atccggtcag tctggactat ccacaagcaa
    13081 aagtgcttgc cgagaaagca gcgcaagcgg gcagtaaaga aggtgaagta acgctggcgc
    13141 atattctggt aaatacgcaa gcgggtaaac cggattatcc aaaggcgatt tcgctgttag
    13201 aaaacgcctc ggaagatctg gagaacgact ctgccgtcga tgcccaaatg ctgcttggtt
    13261 tgatttacgc caacggcgtg ggcattaagg ccgacgatga caaagcaacc tggtatttca
    13321 aacgtagctc tgcgatttcc cgaaccggtt attccgaata ctgggcggga atgatgttct
    13381 taaacggtga agagggtttt atcgagaaga ataaacaaaa ggcgctgcac tggttgaatc
    13441 tgagctgtat ggaaggattt gataccggtt gtgaagagtt tgaaaaatta actaacggtt
    13501 aaggtaggcg cagcaaacgt cgcatcaggc attgatgtcg gatgcggcgt aaacgcctta
    13561 tccgacctac aaaaatcacg ccaaatcagg ctattgcgct gtctgacgta ggcctgataa
    13621 gacacgtcag catcgcatca gacattgatg ccggatgcgg cgtaaacgcc ttatccgacc
    13681 tacaaaaatc acgccaaatc aggctattgc gctgtctggc gtaggcctga taagacgcgg
    13741 caagcgtcgc atcaggcgtt gatgtcggat gcggcgtaaa cgccttatcc gacctacaaa
    13801 aatcacgcca aatcaggcta ttgcgctgtc tggcgtaggc ctgataagac gcgttaagcg
    13861 tcgcatcagg cgttgatgtc ggatgcggcg taaacgcctt atccgaccta caaaaatcac
    13921 gctacatcag gctattactc agtcaggcat ccacacattg ccgggtggat atccccccgg
    13981 caatcttcaa ttactgatct gctgttttat caaatttgcc caacatttca cgctcgtagg
    14041 ccagcgcttt tttacggtca aatttgtgtt cccacttggc aatcaccagc accgccagcg
    14101 cattacccac cacgttcagc gcagtacgcg ccatatcgag gatacggtca acaccggcaa
    14161 taaacgccag accttccagc ggaataccta cgctacccag cgttgccagc agcaccacaa
    14221 acgacacgcc cggcacgcca gcaatccctt tcgaggtcac catcagcgtc agcaccagaa
    14281 tgatttcctg ccagatggac aggtcaatgc catacagctg agcgatgaaa atagcggcaa
    14341 tactttgata cagcgtcgaa ccatcaaggt taaaagagta accggtcggc accacgaaac
    14401 tggtgatcga cgccggtgca ccgtaggctt ccatcttctc aataattcgc ggcagcacgc
    14461 tttcagaact ggcagtagag tacgccagaa tcagctcatc tttcagaata cgaatcagga
    14521 tccagacact taacccgcac aggcgcgcca caattcccag tacgaccagc gcgaagaaca
    14581 gaatggcgaa atgcaccagc agcaccagtt tcgccagtgg ccacagagaa gagaaaccaa
    14641 agttagccac cgtcaccgca atcagcgcaa acacacccac cggcgcataa cgcatcacca
    14701 tgtgagtcac tttaaacatg gtttcagaga tggagcggaa aacggtcacc agcggttcac
    14761 gatgcgtcgc gggcagggat gaaagcccca gaccaaacag caccgagaag aagatgatcg
    14821 gcagcatttc gcctttcgcc atcgacgcca cgatgttcgt cggcaccagc gacaaaatcg
    14881 tgcccataat gccgtgggaa ctgctttgta ccgcttccgt agtgctctga tatttcgaga
    14941 tatcgacggt cgccagctgc gacatatcca ccccggcacc gggctggaag acgttcgcca
    15001 gagtgatccc caaaatgatg gcgacggtgg tgatcacctc gaagtagata atggttttcg
    15061 caccaatacg cccgagctgt ttggcatcac caacacccgc gatacccacc accagcgtgg
    15121 agatcacaat cggcacaaca atcattttga tcagatggat gaagatatca cccgccggag
    15181 agagcaaatt gacgaccagc cagtcgcggc tgtcgctatg gtagtgcagg tagcttccca
    15241 ggagaatgcc cagcaccata gcaaacagaa tctgccaggc caggctgaat tttatatttt
    15301 tcatcagaat aacttcctca atggagcgcc gttccccgtt cagaaacgcg agaacgtgac
    15361 atactgccag ggcatgaatt gtgttgcgtt ggtttgtaga tttttttaac gcttatgatc
    15421 agtatcattt gcgcggtatg ttgcgcaacc ctttaacaac agagcttttc gctgcattaa
    15481 tgcataataa cgcgagttta tggcaggatt aatgaataac catttgttat agaaggattt
    15541 ttatttgttt aatctgcaat aatactgatt agtgattatt tttgattata tgttcattcg
    15601 attatttttt atgcagctca ataacataat cttcatcacc agatgattaa tctgatctcc
    15661 gctgcaacaa tttcgccata ttaatcgact cgaccagctg tgtccggttg atccggggtg
    15721 agttgagatc catcactttt tgccatagtt cgatggcttg cgcgtaattc gcctgcataa
    15781 acgcatcgga agccagcagc atcagagcgg tgatttcgtt agagtccagc gcgagggctt
    15841 tgtcgatcat tgcgcgagcc tggacggtca tatgttggct ggcctggtaa tacagcaccg
    15901 tcgccagcgc cgcatacagt tcagcgttct caccacgtag ttgcaacgcc tgacggtacg
    15961 ccagcagcga attgctgtaa tcgttttgcc acagataata ctcgcccagt aacgcccact
    16021 gttcgctgtt ttgtggatta gcacggattt tatcctgcaa tgcctgaagc tgcgcttccg
    16081 gggtttgctg gctggcaaac tgatgcagcg gatcgcgctg acgctgatac tccgcacgta
    16141 ccgcctgcca ttttggactt aacagataac tgccgacaca gagaaatacc atcaaaatcg
    16201 tgagtgttgt cagcattttc accggtattt ggggctgttt catcgcttcg ccctcactcg
    16261 ccagaggatc agtgccatca gcagtaacaa cactactggc agcgcccaca acaccagagt
    16321 ctgacccgtt aacggtgggt tatagcgaac aaaatctccg tagcgttcgg tcatccagcc
    16381 aatgatttcg acttcacttt tcccctcttc caccatggtg taaacctgat ggcgcatact
    16441 gactgccacc ggcgcattgg attccagtaa gttctgattc tggcactgcg gacaacgtaa
    16501 ctggctggca atatttaacg cctgttcctg ttgttgcgga ttagcgaatt gccaggtgtc
    16561 tacaacctga gcgcgcgcaa aacaggtaaa taacagcagt agcgtgagaa gccctttatt
    16621 catcgcgctt ccttccccgc catccgctta acaatgcgcc cgcaatcatc aacaatcctc
    16681 ccccccagat ccagcgcaca ccgctttgta catacaaacg aaatgcgtaa cgatccgctc
    16741 cggttttctc tcccatgacc gcataccagt catggatgcc gttccagcga atcgagggtt
    16801 ccatcatttg ctgacggcgt gcttcgtaaa aacgccgctc cggcgttaat tcaccaatgc
    16861 gttgctgatg gtcaaacagt gccactatcg ctttttcgct ggtgtaattg cctttggctt
    16921 gcagatcgag gcgctcaaaa cggaaggtgt atcctgccag cgtcacctgc tgacccggct
    16981 gtaaattcag gctgatctcc tgacggctga cgctggaaac cacgatcccc gcggcaaata
    17041 acagcacgcc cgcatgcgcc agcagcgtcg gaagccgcgc gcgtttgcca gagacaaacg
    17101 tcgccagcac aatcaccacc agcatcaaca gagcaaacgg taacgtcgcg cggttaaaat
    17161 acggcgcgcc aacggagagg cgtccccagc ccagcaggcc gtaaatcatc ggataaagcg
    17221 ttcccaccag cacgatcagt agcactgcgc aaaacagcaa cagcgtagcg aggattaaca
    17281 tttcccgcga taaccccgaa aaacgcaccg ccgggccacc gtcccgcgct cgccagccat
    17341 acagagccag agacgcaagg ctaatcagta caaacaggcc gaacaacggc acggcgcgga
    17401 cgttatccag cgcgaacgca tgaaccgaaa ccagaatgcc agaacggaca atcaacgtgc
    17461 ccagcagcga cagcatcaga gtaactatcg tcagcaacag cgaccagtgg cggaaaatcc
    17521 cccgctggcg tgtcagggat aaactgtgca gcagcgctgt ggcagaaagc cagggtagta
    17581 aagaggcgtt ttccaccgga tcccagaacc accagccgcc ccagcccagt tcgcaatagg
    17641 cccaccagga accgaggatg atccccgctg ttaatgcgct ccagccaggt agtgcccaac
    17701 gccagcaaat tcgggcgcag gtaccatcaa actcgccgcg cagtaaactc gccagcgcca
    17761 cgctcgccgc caccatcaaa ccgccgtagc cgagataaag cagcggtgga tgaaagataa
    17821 gaccgggatg ttgcagcatc ggattgagat cgcggccttc gattgctggt ggaaatatcc
    17881 gcacgaaggg atcggaccac agcaccacaa acagtagcag tgcggcgagc atgagagata
    17941 aaacggctag cgtcagcgga aatagcggat cggtttgctg ccggtaatgc caggcaaaca
    18001 gcgcgctcca ggcggaaagc agcagcaccc aaagcaacag cgaaccttca tgaccgcccc
    18061 acaccgccgc cagtttgagt tcccacgaca gcaggctata gctatgttgg gcgacataaa
    18121 taaccgagaa atcgctgatg agaaaacaat acgtcagtac gccaaaggcg agcagcagga
    18181 gcgcgaactg cgccagaatg ccgatgcaag tgagtcgcat catggcaggc cagcgcaacc
    18241 gcactcccgc gaaggccgtc aacgggatca acacgttgac cccgagactt aacaacaacg
    18301 ccagaaaacc gacttcagga aggaatacgt ccagaaacca cctctgttct ggcttacgcc
    18361 accgttaact gcccggcgta gagaatgaaa aagcgcagca tcaacacgcc gaccaggctg
    18421 gccccacatg ccgccagcac ggcaggaatg ccggaactgc gattgaccca cggtttgagc
    18481 aacattggca caatcagccc cagcccggcg acaccaagcc agaaccacca cgtccagaaa
    18541 ccgccgccca atgccgccac cagcgcacgc actttaccgt catcgcccag cgccagaccg
    18601 acaaaaaacg ccaccagcag gaagatttca ccccatacca cggggatttc catacggtgt
    18661 acaaagtgcg cttccgtgga atgcgggtta ctgcgttggc gtatcgccat ggcgatcagc
    18721 gccaccgccg caccggacga gatgccggag aagaggaaca gcaccggcag gatcgggtta
    18781 ttgaggaacg gatacgattt cagcgccgac agcagaaagc cggtataagc ccccaacaac
    18841 accgccagca ccagcatcaa tgtttccagt ccgcgatgaa cgggcgtcag caggctcaag
    18901 accttttgca cgatccccag cttcggcaac cagcgttgtt gcagggcaag caaatcatgt
    18961 tcaaagattt tcgccagcca cagcaccagc accaccatgt aaagctgaaa cagcatcacc
    19021 cccatcgaca tcaccgaggt aaaactgtag tggaacatca gcttccagaa ggtccacggt
    19081 cttgtcaggt ggaagacgag gatcaacaga ccgaggatca ccgcgccagg cccgacaatc
    19141 agcgtggtgc gcagcaacgt actgtctgca ccgcctgcct gcgggtagaa gcgacgtaac
    19201 agcacggcca gtgtcaccag accggcagaa ataccaatca aaaacaggta gatggcaatc
    19261 ggccagtccc acaccagcga ttcaaaatga aatgcggaag tctgcgtcat tggctcacct
    19321 ccccgtattt aaacggtacg cggtacagct tcggtttggt gcccagcgcc agcttgtagc
    19381 ggtaagtggg cttctggcgc agcagttgcg aaatctcact gttgggatcg tccagattgc
    19441 caaacgtcag cgccttcgtc gggcaagctt caacgcacgc gggcagctta ccggcctgca
    19501 aatgggtctt acggcagaaa tcgcatttgt ccgccgtctt cgtgaccgga tggataaagc
    19561 gcacgcggta cggacaggcg gcgatgcagt actgacaacc gacgcagaga tccggattaa
    19621 catcgacaat cccactggca gcgtcgcgaa aagacgcacc agtcgggcag acgtcaacgc
    19681 acggcgcatg atcgcagtgc tggcaagact tacggaagaa gcgatatttc acatcaggaa
    19741 attcgccctg cggctcgcta cgaataatcg tcaggcgcga gacgccttcc ggcaccttgt
    19801 tcactttccg acaagcatcc atacaggcgg tacagccgat gcataacgac tcgtcatgca
    19861 ccatgccgta acgcacccca ttgatattca acgttttcgc caccacgcgc cctgcggtgc
    19921 cgctgacggc ggccagcaca ccgacgccgg tgagaaactg gcgacgagac caggtcatgg
    19981 ctgctcctta agcaacggaa cagacgccgg gttaaagttc ggattggtgc gctgatcgct
    20041 gtggcaatcg acgcaaatct taatccgtcc tttgtcactt aacgtctgca tcgtatcttg
    20101 ctgtggatgc agggaatggc agctggcgca cgccactttg gttacgtgga catcgtgcgg
    20161 ccagaatgct ttttgcaact gttccggcag atggcaggac atacagacgc tgttctgctc
    20221 tccaaccttg tacatcggct cgttaaagcg catcacatct ttcacccctt cgcggtgttg
    20281 cggtgatggc tggccgtgac agttggtgca ggtgaccggc agtttgttat tcgggttgat
    20341 aacggaagca tgtttgccat gcataccttc ggtatctggc ttatgacagt ccagacaggc
    20401 ggcatccggg ttacgctgct gggtaacttc gtagcgatca ccagatgcct gcgccgcagg
    20461 ggtagcggta attccgttca ggctccacaa caggcccgac gccagcaccc cggcagttaa
    20521 taacgaacgt aatacgctca tattcactcc attgtgtttt ccgccccttg cgaggcggaa
    20581 cggggttatt ggcttaacag accgttttta cgtgcctgct cttcccactg cgggatcacc
    20641 gttttgatga agtcctgctt ctcggccttg atttgctcca tgttcaggcc aatggcctgc
    20701 tgggctttct ctttggtcga gatatccggg atctggattt catgggtaat gcctttggtc
    20761 gccagcaggc gcgccagttt ggtgcgtgca tccgccgctt tatccatcgc cgtaccgagc
    20821 atcctcagac cttcttccgg tgcgtgcata tgaatgccgt gggaagcgat cgccaaatcc
    20881 caacgccact gggcatgacg gatatcgtcc tgaatcggct tcatttcggc ttccgttgcg
    20941 cctgcatcca gcgccgcttt cgcttcgaag tgagcgtgaa ccagttgatc ttcaaccttg
    21001 attttcaggt cgttaatcga ctgcttacgt tccgcgacca ctttttgcaa ggcagctttg
    21061 tcctgggtat ggcagttcgc acaagtctgg gcgaagttat caaacggatt accaatttta
    21121 tggtcggtgt agagtttgcc ttcggcgttc tgcacttttg gcatatggca atcgatacag
    21181 gtcacgttgt ttttgccgtg aatgcccgct gtccaggttt catattccgg gtgctgcgct
    21241 ttcagcattg gcgttttcga cagggagtta gtccagtcag agaaggcaat tttgtcgtaa
    21301 tactgctcca tattttcgac tttcatgccg tcatcccacg ggaatttaac cgctttgttt
    21361 ttgccgtcga agtaatactc cacatggcac tgaccgcaaa ccatcgattg ctggtcgaaa
    21421 cgtccggctt tctcaaaagg tttaccaatg gcttccatcg cgcgagccgc atacggacgg
    21481 gaaagggtta actccggttt gcctttggcg aactctggtg aggcggtgtt atggcaatcg
    21541 gcacaaccta agttgttgac gatttccggg ccgccgcgcg cccatttacc gtggaagtag
    21601 ccatcttcgc cgtctttctg gatcagacgc gccacatccg ggcttttaca actccagcac
    21661 gccatcggta gcggaccatc ttcagcgttt ttcggcgcac cggtacgcaa ggtttcacgc
    21721 acatcggtca cagcaaaggc atgtccacgc ggcttgttgt aatcgcgcga gaagggatac
    21781 cccgcccaca ggatcaccag ccgtggatct tccgccaggg cgtcaacacg ctctgactgt
    21841 tccgaggtag ctttccagga gagatattga tcgggatgct gcggggcaaa ggtttcattc
    21901 ttcgcttcca cagttacagg tgttgcggga gcagccgttt gttcagcgtg aacagaagtg
    21961 aaaaagaaaa aaggaatcaa taagctgaag atacggcgtg cgtttatttt tatccttgtc
    22021 atagggcttc atccgaattg agccattgtt gcaatggcgg tttttattgt ttttcacgac
    22081 agtaaccgca cctacactgt catgacattg ctcgccccta tgtgtaacaa ataaccacac
    22141 tatgaatgtt gtctttaatc aattgtaagt gcatgtaaaa taccacttta gagttagtcg
    22201 gtatcttcct ctttttcaac aacatgcata actgcatgtt cctcaaagaa ttaatcaact
    22261 tttgttgctg accttcaaaa attaccctgc cgtttatttg cacaattcta cttttgcgtg
    22321 atctgtcgcc caaatactaa acaaaactgc caatacccct acatttaacg cttatgccac
    22381 atattattaa catcctacaa ggagaacaaa agcatgagtc aaattcacaa acacaccatt
    22441 cctgccaaca tcgcagaccg ttgcctgata aaccctcagc agtacgaggc gatgtatcaa
    22501 caatctattg acgcaccaga taccttctgg ggcgaacagg gaaaaattct cgactggatc
    22561 acaccgtacc agaaggtgaa aaacacctcc tttgcccccg gtaatgtgtc cattaaatgg
    22621 tacgaggacg gcacgctgaa tctggcggca aactgccttg accgccatct gcaagaaaac
    22681 ggcgatcgta ccgccatcat ctgggaaggc gacgacgcca gccagagcaa acatattagc
    22741 tataaagagc tgcaccgcga cgtctgccgc ttcgccaata ccctgctcga actgggcatt
    22801 aaaaaaggtg acgtagttgc gatttatatg ccgatggtac cggaagccgc ggtcgcgatg
    22861 ctggcctgcg cccgcattgg cgcggtacat tcggtgattt tcggcggctt ctcgccggaa
    22921 gccgttgccg gacgcattat cgactccagt tcacgactgg tgatcacttc cgacgaaggt
    22981 gtgcgtgccg ggcgtagtat tccgctgaag aaaaacgttg atgacgcgct gaaaaacccg
    23041 aacgtcacca gcgtagagca tgtggtggta ctgaagcgta ctggcggaaa aattgactgg
    23101 caggaagggc gcgacctgtg gtggcacgac ctggttgagc aagccagcga tcagcaccag
    23161 gcggaagaga tgaacgccga agatccgctg tttattctct atacctccgg ttctaccggt
    23221 aagccaaaag gcgtgctgca cactaccggc ggttatctgg tgtacgcggc gctgaccttt
    23281 aaatatgtct ttgattatca tccgggcgac atctactggt gcaccgccga tgtgggctgg
    23341 gttaccggac acagttattt gctgtatggc ccgctggcct gcggcgcgac cacgctgatg
    23401 tttgaaggcg taccgaactg gccgacgcct gcccgtatgg cgcaggtggt ggacaagcat
    23461 caggtcaata ttctctatac cgcgcccacg gcgattcgcg cgctgatggc ggaaggcgat
    23521 aaagcgatcg aaggcaccga ccgttcgtcg ctgcgcattc tcggttccgt gggcgagcca
    23581 attaacccgg aagcgtggga gtggtactgg aagaagatcg gcaacgagaa atgcccggtg
    23641 gtcgacacct ggtggcagac cgaaactggc ggtttcatga tcacaccact gcctggcgct
    23701 accgagctga aagccggttc ggcaacacgt ccgttcttcg gcgtgcaacc ggcgctggtc
    23761 gataacgaag gtaacccgct gaagggggcc accgaaggca gtctggtgat caccgactcc
    23821 tggccgggtc aggcgcgtac gctgtttggc gatcacgaac gttttgagca gacctacttc
    23881 tccaccttca aaaatatgta tttcagcggc gacggcgcgc gtcgtgatga agatggctat
    23941 tactggatca ccgggcgcgt ggacgacgtg ctgaacgtct ccggtcaccg cctggggacg
    24001 gcagagattg agtcggcgct ggtggcgcat ccgaaaattg ccgaagccgc tgtggtcggt
    24061 attccgcaca atattaaagg tcaggcgatc tacgcctacg tcacgcttaa tcacggggag
    24121 gaaccgtcac cagaactgta cgcagaagtc cgcaactggg tgcgtaaaga gattggcccg
    24181 ctggcgacgc cagacgtgct gcactggacc gactccctgc ctaaaacccg ctccggcaaa
    24241 attatgcgcc gtattctgcg caaaattgcg gcgggcgata ccagcaacct gggcgatacc
    24301 tcgacgcttg ccgatcctgg cgtagtcgag aagctgcttg aagagaagca ggccatcgcg
    24361 atgccatcgt aacccacaat tgtcggatgc gctccgacct acatctggca acggctgtag
    24421 gtcggataag atgcgccagc atcgcatccg acaacaagcg ccggatgcgg cgtgaatgcc
    24481 ttatccggcc tacattcggc aagggttacc caagcgttaa ccttctccca taagggagcg
    24541 ggaattaaaa caatccctac attacctctg gagaatctgt gatgaatggt acgatttatc
    24601 agcggataga agacaatgcg catttcaggg agttagtcga aaaacggcaa cggtttgcca
    24661 ccatcctgtc gattattatg ctggcagttt atatcggctt tattttactg atcgccttcg
    24721 cgcccggctg gctgggcacc ccgctgaatc cgaacaccag cgtcacacgc ggtattccag
    24781 tcggtgtcgg ggtaattgtg atctcctttg ttctcactgg tatctacatc tggcgggcga
    24841 acggcgaatt cgaccgtctt aataatgaag tcctgcatga ggtacaagca tcatgaaaag
    24901 agttctgacg gcgcttgccg ccacactccc tttcgcagct aacgccgcgg atgctattag
    24961 cggggccgta gagcgccagc caacgaactg gcaggcgatt attatgttcc tgattttcgt
    25021 cgtgtttacg ctcggcatta cctactgggc gtcaaaacgc gtacgttcgc gtaatgacta
    25081 ctacaccgca ggcggcaata tcaccggttt tcagaacggg ctggcgattg ccggggacta
    25141 tatgtccgcc gcctcattct tggggatctc cgcgctggtg tttacctccg gctatgacgg
    25201 cttaatttac tcgctgggct tcctggtggg ctggccgatc attctgttcc tgattgccga
    25261 acgtctgcgt aacctcggtc gctacacctt tgccgatgtg gcctcttacc gtctgaaaca
    25321 agggccgatt cgtattcttt cggcctgtgg ttctctggtg gtggtggcgc tttaccttat
    25381 cgcccagatg gtgggcgcag gtaaactgat cgagctgctg tttggcctta actatcacat
    25441 tgcggtagtg ctggtcggcg tgctgatgat gatgtacgtc ctgttcggcg gcatgctggc
    25501 gaccacctgg gtacaaatta ttaaagccgt gctgttgctg ttcggtgcca gctttatggc
    25561 ctttatggtg atgaaacacg tcggctttag cttcaacaat ctgttcagcg aagcgatggc
    25621 ggtacacccg aaaggtgtcg acatcatgaa gccgggcggg ctggtgaaag atcctatctc
    25681 cgcgctctct ttggggctag gactgatgtt tggtacggcg ggcttgccgc atattctgat
    25741 gcgcttcttt acggtcagcg atgcccgcga agcacgtaag agcgtgttct acgccaccgg
    25801 atttatgggc tacttctaca tcctgacctt tattatcggc tttggcgcga tcatgctggt
    25861 cggtgcgaat ccggaatata aagacgcggc gggccatctg attggtggta acaacatggc
    25921 agcagttcac ctggccaatg cggtgggcgg caacctgttc ctcggcttta tttcagcggt
    25981 tgctttcgcc accattcttg cagtggttgc gggtctgacg ctggcgggcg catcggcggt
    26041 gtctcacgac ctgtacgcta acgtcttcaa aaaaggtgcg accgaacgtg aagagctgcg
    26101 ggtatcgaaa atcaccgtac tgatcctcgg tgtgattgcc attatcctcg gcgtgctgtt
    26161 tgagaatcag aacatcgcct ttatggtggg gctggcgttt gccatcgcgg cgagctgtaa
    26221 cttcccgatc attctgcttt ccatgtactg gtcgaaactg actacccgtg gcgcaatgct
    26281 gggcggctgg ttaggactga ttactgcggt ggtgctgatg atcctcggcc cgacgatttg
    26341 ggtacagatc ctcggtcacg aaaaagccat tttcccgtat gaatacccgg cgctgttctc
    26401 tatcagcgtg gcattcctcg gtatctggtt cttctcggca acggataatt cagcggaagg
    26461 tgcgcgcgag cgtgaactgt tccgtgcgca gtttatccgc tcccagactg gctttggcgt
    26521 tgagcagggc cgcgcgcatt aatctctccc cttccccggt cgcttgatcg gggaatttct
    26581 tcagaacatc atcctggcca ccagcggcga aaccaatacc atcaatacac ctgaaagcat
    26641 catgaccagg cttgcaacta cgccttcttg ttgacccagt tcatacgacc gcgcagtccc
    26701 ggcaccatga gatgccgcgc cgaatccagc acctttcgcc atcccttcgc gaatagagag
    26761 acgtaaaaac aacacgtcgc caatcgccat gccaaacaca ccggtgacca ccacaaacag
    26821 cgccaccaga tcaggctgtc cgccgagcga ctggctcgct gccaaagcaa atggcgtagt
    26881 gaccgaacgc accgccaggc tacgctggat ctcatctggc agggtaaaca atcgcgccag
    26941 ccacactgag ctggtcactg ccaccaccgt tgccgtcagt acgcctgccg tcaacgacat
    27001 ccagtgacgc ttaatgatcg cccgattgtc atacactggt accgcaaagg cgatggtggc
    27061 cggacctaat agccatagta accagtgcgc ttcgccaata tagttttgcc aggaaatatt
    27121 gccaaaaacc agcagcataa ccagtagcgt aggcgtcagc accagaggca tcaatggcaa
    27181 tttacgaaag cggcgataaa ggcgtttgtt aagaaaatag ataactagcg taattgccag
    27241 acagagcata cttagctgaa aattagccac ggtttaacct gcgcatttca tagcgataga
    27301 ctttttccac tacccaggca gtcgtgccca gcaccatcac cgtactcagc gcgatcacag
    27361 caaaaatacg ccagccatcc accagtaata gttgtgcata gttcaccaca gccaccacgg
    27421 cagggacaaa aaacagcaac atttcggcca atagccaacg cgccccggct cgaacccatt
    27481 gcagaggaag aatgcgacaa acaatcagcg ccagcatcag gatcatcccc accaggttgg
    27541 caggcagcgg caaatgcagc caggagacga gatactgcgc aaaaataaat aaacctgcat
    27601 acagcacaac ctgaaaaaat acctggatag attgcactac ggcaggggta atgcggcgaa
    27661 gcgccacggg catgagagga tatcctgaaa ataaaacaga atggtaagta tagagagcgc
    27721 tcgcccctga ctgaaatgaa ttaaaatcat tgcaggtata gtcaaaggga atagttatgg
    27781 atatcagaac gctgcgttat tttgtcgaag tcgtgcgcca gcaaagcttc acccgcgcag
    27841 cggagaagtt gtttgtcacc caaccaacta tcagcaagat gctgaagaat ctcgaagatg
    27901 agctgaactg tatattgttg atccgcgatg gtcgtcggct gcttctcact gatactggtc
    27961 gcgtcgtatt tgagcgcggg ctggctttac tggctgagtt tcggcaactg gaagctgagc
    28021 ttagcgatat caatcagctt aaaaccggcg ttttgcggct gggaatcccg ccgatggttg
    28081 ggatgttgat ggccgggccg attggccttt ttcgccagcg ttaccccggc gtagaattga
    28141 aaatttcgga gtttggtggt ctgacggtac aacaagcggt gatgaacggt gaactggata
    28201 tggcactgac agccctgcca gtagaagaaa gcggcctgac aacgttatcg ctgtttagtc
    28261 atccactatg tgtactgacg ccacattcag gtaaatggct tgaatgtgat gccgtctcac
    28321 cggagacatt ggcggaacat cctctactta tctataacga agattttgcc ttaagtaagc
    28381 aattgatgaa gttattcgat gaacatggcg ttaagccgag aattgcagtt cgcagcgggc
    28441 aatgggattt tctcgccgcg atggttcagg ccggtgtcgg aatagccata ttgccggaac
    28501 ccatttgcca gcgattagat aaaaacacct taaaatggct accgctggaa agcgaactaa
    28561 gctggaaatt aggaatgatt tggcgtgagg gcgtttacct ttcccactct gcccacgcgt
    28621 ggctgagctg ttgtgaagga tactggttaa cgccagagta agtcgggttg gcgggctcgc
    28681 ccgccaacca tgcagattac tgattttcct caatcagcaa cgcttcaagc aaatcgagat
    28741 cgtgcagtaa tttttgcagc gtttcgttac tgatctcccg cgtggcgcgc aggtggtaca
    28801 gttcagcacg ttcagaacgc aatgccgcca gacggaagcg acgctccagg ttctcttcca
    28861 ggattgaact ttctacatca ttacgtccat cggcgcgacg acgcaggtta ccaatgacgc
    28921 gagaactaac ctctgtgagc agctggttat cgatattctc ttcggtatcc gccgccagac
    28981 gctcctccat tttctggatc gccacaatcg ccacttctgc cgttgccgct cgtgcaatac
    29041 gttcctcttt caattgttgc gaatggtccg cgacttctat gtgttgtagc agaataggca
    29101 acatcaccac gccgacaaac aatgagaaga gaatgacgcc agccgccagg aacaccaact
    29161 catagcgcgc agggaagacg ttaccatccg gcaagagaag cgggatagag agcacaccgg
    29221 ccagagtgat cgccccacgc accccggcga aagacgcgat caggatttct cgtgtagtcc
    29281 acgaaccaaa ctccatcggc ttcttcttca ggaagcggtt gctgaacttt ttcatcgtcc
    29341 acagccagcc gaaacggacc agcatcagcg ccgcatatat cagaataata tcggtaaaca
    29401 gcatccagat ttcgacgtta ggatcgattt ctgccgccat cagcgacgtc tccagaatcc
    29461 ccggcaactg cagacctaac agcaggaaca ccatgccgtt aaagacaaat tccagcatcg
    29521 cccaggtgct gtttgcgcgc aggcgcattg ccagcggcgc acggcgcatc acaccggagc
    29581 gggtgatggt catcccggcg gcaaccgcag cgaggatgcc ggaaacgcca atatgttccg
    29641 caatcagata agaagcgaat ggcagcaaga acagcagaac gatctgcgtc gcgggttcat
    29701 caccgcccca gcggctgagg aatcgcagcg agcgaccata cagccagctc accacaaaac
    29761 cggcgagaat accgcctatt gccactttca taaattcgac cgtcgcaccg ccgacggtga
    29821 agatcattgt ccccatcgcc actgccacgg caaacttcaa cgacaccagg ccggaggcgt
    29881 cattcatcaa cgcttcgccc tgcaaaatgc ccatgatttt tttcgggatg cgcccctcgc
    29941 caacaatccc ggagagcgct acagcatcgg tcggagaaag caccgcagcc agtgcaaagg
    30001 cggggatcag cggaatgccc ggcaccaccc agtaaataag gaagccaatg ccgaccacgg
    30061 tgaccaccac cagcgccagc gcgaggccga aaatctctcg cccatgttcg agaaattcac
    30121 gggtcggcgt tttccaacca tcagcgaaca gcaacggcgg gataaacaag actaaaaaga
    30181 gttcaggatc aaactccaca tgcaaaccaa acgtcggcca cgccagtagc gcaccgatgg
    30241 cgatttgcat aagcggaagc gggatctgaa agggcatgac acgagtgacc accccggaca
    30301 gcgagaccac gagggtcatt atcagtatgg tgaagaagat ttccatgcgt tccctgtttg
    30361 cgatgttttt tatgatctga gaaaggcgtt atgcctgaat tctcattttt tcagaatatc
    30421 gcaaaagctc acacaatgtg cggagaaaat aaaaacgggc agccattgcc acccgttttc
    30481 agaatcctat gtaagattag atagcccagc cacccgcata gaaggtcacc agcgccacgg
    30541 caataacgac cgtaccgatg ttcaacttgc gccattcgcc ggaaaccaga cgaccaatca
    30601 ccagagtcgc gaagccgatc atgatgcctg ttacgatgtt acaggtcagc acgatgaata
    30661 ccgccgtaac cagacccgcc atcgcatcaa caaaatcagc aaagtcgatt ttcgccacgt
    30721 tgctcagcat cagcaggcca acgtacatca gcgccggagc cgttgcgtac cccggaacga
    30781 ggtaagagag cggagagagg aacagaatca gcaggaacag cacgccaacg gtgatagccg
    30841 tcaaaccggt tttaccgccc gccgccgtac ccgccgcaga ctcaatgtat accgctgccg
    30901 gagctgcacc caccaggcca gagaaaacgc tgctcatgga gtcggtggtc agtgctttcc
    30961 cgccgtcgat gatctgccca tctttatcca gcaggttcgc ctgaccggcg acggcacgga
    31021 tagttccggt ggcatcaaat actgccgtca tcaccagtgc cagaacactt ggcaggacta
    31081 caggattcag cgcacccata atgtccaggc tgccaatcag tgaattgccg ttttcatcgc
    31141 tcaatgaagg catggcgaaa acgccggaga aatggacgtt aggatcgaag atcaaaccga
    31201 caattgagat accgataatg gtcagcagaa tgccgccagg gactttcagt ttttccaggc
    31261 cgataatcac tgccagcccc accaacgaca tcatcaccgg aaaggtcgta aaatccccca
    31321 gcgcgacggg cagaccatca agcgggtttt taatcaccag accgacacca ttagcggcaa
    31381 tgagcagcag gaacagaccg ataccaatcc ccgtgccgtg cgccacaccg tgcggcaagt
    31441 tgcgcaaaat ccagctacgg atacccgtgg cagaaattac cgtaaacagc acacccatca
    31501 ggaacacggc acccagcgcg acaggtacgc taatatgttg ccccagcacc agactgaatg
    31561 cggtaaacgc cgtcagagag atggcgcaac caatcgccaa cggcagatta gcccacagac
    31621 ccatcacgat agaaccgagt ccggcaacca gacaggttgc aacgaaaact gccgcaggcg
    31681 ggaagcccgc tttacccaac atgcctggaa cgacgataac cgagtagacc atcgccagaa
    31741 acgttgttaa cccggcaacc acttcctgac ggacagtgct tccacgttgt gaaattttaa
    31801 accaggcgtc gagtgaaccg ccggtacgcg ctgatggcgt agacataaga aaacatcccc
    31861 tgagagttta attttcgtca agatgcgtgg cggacaatcc cctgccagcg aaacgttatc
    31921 ttcctgctcc aggtttgcga caatttgctg cgtcgcaaaa aaagcaaacg tttagctccg
    31981 cgcatacaaa acgggtggta aaaaaagcaa acgattatcc ggcgatcata gcggcttttt
    32041 caaccgaagt ttgctgcttt tttctctttt attgtcttga cgatgaaaaa ccaggcaatt
    32101 gatgcgcaaa cgttatcgtc gatgcgcaag attgcagcgt tatcgagccg tataccgttc
    32161 aggattgcga taaaaaatcg tctgagatga caaaaatatt gcggctaaaa agtgtacttt
    32221 tacactggcg aagaaacgcc ggtaacatac aattaaagca tcaacaccaa ccggaacctc
    32281 caccacgtgc tcgaatgagg tgtgttgacg tcgggggaaa ccctcctgtg taccagcggg
    32341 atagagagaa agacaaagac cggaaaacaa actaaagcgc ccttgtggcg ctttagtttt
    32401 gttcatcttc cagtaagcgt gcgccggtac cttcttctcc taagcggtcg cccgggttac
    32461 gcaacgggca atcactgcgc gaaaggcagc cacaaccaat acatccatcc agttcgtcac
    32521 gcagcgccac taaggtatga atgcgccgat ccaactcttc tcgccattgg gacgaaagct
    32581 gtttccactc tttcgcactt aacgtatgcc cttctggcaa cacgccaaac gcttcacgaa
    32641 tggttgcgag cggaatgcca atacgctgag caattttgat aattgcgaca tatcgcaaca
    32701 catcacgttt atatcgccgc tgattgccgc tattacggat actggtaatc aaccctttgc
    32761 tttcatagaa atgcagcgcc gataccgcca caccgctgcg tttcgccact tcgccgggtg
    32821 ttagcagcgc tttaatgcgg ggtaatttct tttccataaa tcgctttacc tcaagttaac
    32881 ttgaggaatt atactcccca acagatgaat taacgaactg aacactgaaa agaggcagat
    32941 ttatgtccca tcagaaaatt attcaggatc ttatcgcatg gattgacgag catattgacc
    33001 agccgcttaa cattgatgtc gtcgcaaaaa aatctggcta ttcaaagtgg tacttgcaac
    33061 ggatgttccg cacggtgacg catcagacgc ttggcgatta cattcgccag cgccgtctgt
    33121 tactggccgc cgttgagttg cgcaccaccg agcgtccgat ttttgatatc gcaatggacc
    33181 tgggttatgt ctctcagcag accttctccc gcgttttccg tcggcagttt gatcgcactc
    33241 ccagcgatta tcgccaccgc ctgtagtttt attgcccgcg cgttaattcc cgcgcgggta
    33301 attgctccat ccattgcata aacacctgcg gcggcatcgc cttcgcgaag aaccatccct
    33361 ggcaatagcg cacgccgcgt ttgcgcagcc agttaacctg cgcctcagtt tcgacacctt
    33421 cagcaatcgt ttttaacccc aggctgtgcg ccagctcgat gatgtgttcc gcaatcaaat
    33481 gactggtttt gtgggtggtc agcgtctcaa caaacgattt atcgattttc aaaatatcga
    33541 cattcaatga tttaaggtta tgcaagttag agtagccaat accaaaatca tcaattgcca
    33601 cttcgtaacc ggccttgcgg aaagccagaa taatcggcgt cattttgtcg acatcaagaa
    33661 atgcatgttc agtcacttca aatttaattt gctgcgggcg caccgcatgt tgctctgttt
    33721 tctgattggt tcgcgctatc aaccgtgagg tatgaaaatc ggaagctgac aggttaatag
    33781 aaacatagcg atcgttatgt gttgccagat acgcgcccaa atcacggaag acattatcaa
    33841 taacataatc agttacctgt tcgatcatcc cctccttttc tgccagcgga ataaactctg
    33901 ccggattcat tacttgcccc tgctcaccgg gccaacgtaa caacgcttca gcgccgatac
    33961 atttttctgt tttgatatcg attattggct ggtaataaag gcaaagttga tgtttttcga
    34021 gggcgcgttg caatttgcgt ttgggagaca aatagttttg tcgaatacgt agccagagta
    34081 atagcagaac aagactcccc aaaataccgg cgggcaacgc aaaaataaga tgattataaa
    34141 aatgggttat aagacgttga tatgaagtcg caacaatggc cgcaattggg cgttttgtcg
    34201 aataaactgt cgcatataaa tagccatttc gttgtacggt taaatcattc aaacgaatca
    34261 gcggagaaaa cgttgctgcc gaggcctcgt tgcttaacga gaaaaaggtt ttcgtcaccg
    34321 tatcatacac accccattgc aatgtcggat catcagacat cacttcactc cagaagagag
    34381 ggttaatgac cgctacataa ttcccccgct gcatataggt cattttatag cctgagaaaa
    34441 aaggcgtatc acggtaataa tagatagaaa cgttaggttc acgcttataa tcggccggtg
    34501 caatcgtata gccatttaca gacgctatca gcgatgagca taaaaaatgg ttatcacgag
    34561 cataaatcaa ttcattaata taaagatagc cacgaataat attcaacatt cgcttttgat
    34621 gggctggagt acaaacttgc ccctgatagc gttcagctgc atcgctcact aaatctgcct
    34681 gacgaatgac cagctcagat ttatccaacg ccagctgagc aaaagttcgg agctgattat
    34741 tcacttctga ttttgcccaa aagaaggcaa tccatagcga cagaatgatg ggaaagagaa
    34801 ctaaaaagat aatgcccgga aacgccagta attggtgtcg tgcacgatga ctcattgcta
    34861 tcaattccct gcctgtttat acccggttat tgcaaggtga aaccctggct accgacgtct
    34921 ttagataaat cttacggtat aaatagcact tttgcctgat ataaaattga gaccttttta
    34981 atcgtcacca ggatgacgat aattcattga tcttaaaaat taatcacctt cagaaatgca
    35041 gaggcggcgg gaagatatac ccttaatgtc gctgaaaaaa cacataatct gtacgtcaat
    35101 aaaagtcacg gcctaatctg gctaacaaac aaccaattgt gatatagttc acaaaattca
    35161 tgaaacaaac agagtgtttc atttttgtgt tctgcaagcc ccgcgatagc cagagtgtat
    35221 cacgcctccc gtgaacaacg ccgcgctgtc cagggcatca gctcttttcc gaggaattgt
    35281 ttgatggcta ccctcaccac tggcgtggtt cttcttcgct ggcaacttct tagtgccgta
    35341 atgatgtttc tggccagcac gctcaacatc cgttttcgtc gggctgatta tgtcgggctt
    35401 gcagtgatca gcagcggtct gggcgtagtt tccgcctgct ggttcgcaat ggggttgctt
    35461 ggcatcacaa tggcggatat caccgccatc tggcacaaca tcgagtcggt gatgatagaa
    35521 gagatgaatc agacaccgcc acaatggcca atgattttga cttgatatgt agaagcctcc
    35581 ataacggagg cttctttttt atggctggca gatgctttaa tcatccacct taaaacaata
    35641 taccctattg ttttaaagac aaatcagaac ggaatatcgt catcaaagtc catcggcggc
    35701 tcgttagacg gcgctgccgg agcggactgc tgcgggcgag actgcgcgcc gccgctgaac
    35761 tgattgccac cctgcggctg ctgaggctga ccccaaccgc cctgcggctg accaccaccg
    35821 atattgccac ctgcaggagc gccaccaccc tgacgaccac ccagcatctg catggtgccg
    35881 ccaacgttca ccacgacttc tgtggtatag cgatcctgac cggattgatc ggtccatttg
    35941 cgggtacgca gctgaccttc gatataaacc tgagaacctt tacgcagata ttcgctggct
    36001 acttctgcca gtttgccgaa cagcacaacg cggtgccatt cagtctgctc tttcatctcg
    36061 ccggtggctt tatcacgcca ggattcggaa gtagccagcg taatgttggc aactgcgcca
    36121 ccatttggca tgtagcgtac ttccgggtcc tgacccagat taccaacgag aataaccttg
    36181 tttacgcctc tgctggccat gttcgtgtct cctgaaaaaa aatcgttctg aataagtgta
    36241 aacgcgcgat tgtaccatta ccaatagcgc ttttactatg ttgtgacctc ggttccggga
    36301 aacaaacctg gccagacatt gttacacaac actccgggta atgcattcca atactgtata
    36361 ttcattcagg ttaatttgtg tcataattaa ccgtttgtga tcgccggtag caccatgcca
    36421 ccgggccaaa aagcgtttaa tccgggaaag gtgaatggat aagatcgaag ttcggggcgc
    36481 ccgcacccat aatctcaaaa acatcaacct cgttatcccc cgcgacaagc tcattgtcgt
    36541 gaccgggctt tcgggttctg gcaaatcctc gctcgctttc gacaccttat atgccgaagg
    36601 gcagcgccgt tacgttgaat ccctttccgc ctacgcgcgg cagtttctgt cactgatgga
    36661 gaagccggac gtcgaccata ttgaggggct ttctcctgcc atctcaattg agcagaaatc
    36721 gacgtctcat aacccgcgct ctacggtggg gacaatcacc gaaatccacg actatttgcg
    36781 tctgctgtac gctcgcgttg gtgagccgcg ctgcccggac cacgatgtac cgctggcggc
    36841 gcaaaccgtc agtcagatgg tggataacgt gttgtcccag ccggaaggca agcgtctgat
    36901 gctgctcgcg ccaatcatta aagagcgcaa aggcgaacac accaaaacgc tggagaacct
    36961 ggcaagccag ggttacatcc gtgctcgtat tgatggcgaa gtctgcgatc tttccgatcc
    37021 gccgaaactg gaattgcaaa agaaacatac cattgaagtg gtggttgatc gcttcaaagt
    37081 gcgtgacgat cttacccaac gtcttgccga gtcgtttgaa accgcgctgg agctttccgg
    37141 tggtaccgcg gtagtggcgg atatggacga cccgaaagcg gaagagctgc tgttctccgc
    37201 caactttgcc tgcccgattt gcggttacag tatgcgcgag ctggaacctc gcctgttttc
    37261 atttaacaat ccggcgggag cctgcccgac ctgtgacggt cttggcgtac agcaatattt
    37321 cgatcctgac cgcgtgatcc agaatccgga attgtcgctg gctggcggtg cgatccgtgg
    37381 ctgggatcgc cgcaacttct attatttcca gatgctgaaa tcgctggcag atcactataa
    37441 gttcgacgtc gaagcgccgt ggggcagcct gagcgcgaac gtgcataaag tggtgttgta
    37501 cggttctggc aaagaaaata ttgaattcaa atacatgaac gatcgtggcg atacctccat
    37561 tcgtcgtcat ccgttcgaag gcgtgctgca caatatggag cgccgttata aagagacgga
    37621 atccagtgcg gtacgtgaag aattagccaa gtttatcagc aatcgtccgt gcgccagctg
    37681 cgaaggaacc cgtctgcgtc gggaagcacg ccacgtgtat gtcgagaata cgccgctgcc
    37741 cgccatctcc gacatgagca tcggtcatgc gatggaattc ttcaacaatc tcaaactcgc
    37801 aggtcagcgg gcgaagattg cagaaaaaat ccttaaagag atcggcgatc gtctgaaatt
    37861 cctcgttaac gtcggcctga attacctgac actttcccgc tcagcagaga cactttccgg
    37921 cggtgaagcc cagcgtattc gtctggcgag ccagattggt gcaggcctgg ttggcgtgat
    37981 gtacgtgctg gatgagccgt ctatcggcct gcaccagcgc gataacgagc gcctgctggg
    38041 tacgcttatc catctgcgcg atctcggtaa taccgtgatt gtggtggagc acgacgaaga
    38101 cgcgattcgc gccgctgatc atgtgatcga tatcggtccg ggtgcgggtg tacacggcgg
    38161 tgaagtcgtt gcggaaggtc cgctggaagc aataatggcg gtgccagagt cgttgaccgg
    38221 gcagtacatg agcggtaaac gcaagattga agtgccgaag aaacgcgtac ccgccaatcc
    38281 agaaaaagtg ctgaaactga caggcgcacg cggcaacaac ctgaaagacg tgacgctgac
    38341 gctgccggta ggtctgttta cctgcatcac aggggtttca ggttccggta aatcgacgct
    38401 gattaacgac acactgttcc cgattgccca acgccagttg aatggtgcga ccatcgccga
    38461 accggcaccg tatcgcgata ttcagggact ggaacatttc gataaagtga tcgatatcga
    38521 ccaaagccca attggtcgta ctccgcgttc taacccggcg acctataccg gcgtgtttac
    38581 acctgtgcgc gaactgtttg cgggcgtacc ggaatcccgt gcgcgcggct atacgccagg
    38641 acgtttcagc tttaacgttc gcggcggacg ctgcgaggcc tgtcagggcg acggcgtgat
    38701 caaagtggag atgcacttcc tgccggacat ttacgtaccg tgcgaccagt gcaaaggcaa
    38761 acgctataac cgtgaaacgc tggagattaa gtacaaaggc aaaaccatcc acgaagtgct
    38821 ggatatgacc atcgaagagg cgcgcgagtt ctttgatgcg gtgccagctc tggcgcgtaa
    38881 gctgcaaacg ttgatggacg ttggcctgac gtacattcgc ctcgggcagt ccgcaaccac
    38941 actttctggt ggtgaagccc agcgcgtgaa gctggcgcgt gagttgtcaa aacgcggcac
    39001 cgggcagacg ctgtatattc tcgacgagcc gaccaccggt ctgcacttcg ccgatattca
    39061 gcaactgctc gacgtactgc ataaactacg cgatcagggc aataccatcg tggtgattga
    39121 gcacaatctc gacgtgatca aaaccgctga ctggattgtc gacctgggac cggaaggcgg
    39181 cagtggtggc ggcgaaatcc tcgtctccgg tacgccagaa accgtcgcgg agtgcgaagc
    39241 atcacacacg gcacgcttcc tcaagccgat gctgtaatcg ttaaggccgc tttcagagcg
    39301 gccttcttct ttcagagttg caccagcaat ttacgttttt cttccggcag caagttcacc
    39361 gcctgctgat aagacgcatc caccagataa tagatttgcg aatccggcag cgatccgtcg
    39421 agatacacgg tgctccagtg tgctttgttc agatggcggc ttggacgcac atcgctgtgc
    39481 tgctgccgta gcagctcagc cagctccggg ctggttttca gcgaaacagc tgggcgattt
    39541 tctacttctt tcaccatcgc aaacagcaca tcttccactt tgatctgcgt cgctttccag
    39601 tcgttatgca cgctctgttc tgcgcctggt tttgccatgc aatattgtag caactccgaa
    39661 atggtcattt ttcactcccc ttgtagtgtc gcgatgatgc gacgcgatcc gccgtggatg
    39721 cgatgttccc ccagccagat gccttgccag gtgccggtct gaatacgccc tttatgcacc
    39781 ggcaatacaa gcgatgttcc cagcattgag gatttgatat gagaaggcat atcgtctgct
    39841 ccctcatagt catgctcata atttccgttg tcgggaacgg cgcggaggaa aaaacgctcc
    39901 atgtcgtggc gtacggtggg atcgcagttc tcattaagtg tcagagaggc ggaggtatgt
    39961 tgcagcaaca gatgcagtaa accgatgtta acgcgcggca tatcagccag ctgattcaga
    40021 atttcatccg ttaccagatg aaacccacga gatttggcgc taagcgtgag cgtcttttga
    40081 taccacatgt gctgctcctt gataaaactc tcttaatcag tttgcagcaa gacagcgaca
    40141 ggataaaggt gtgattaaaa aaacagcatt cgagagagta ttgcgctctc ccgctctgtc
    40201 agtattctga attgacgatc acctcttcac caaacgcacc cgcttgtggc aagggtttgt
    40261 aggtagagtt ggaggcgcgc agaatgcgga taccacgagc gccaacatcg cgtgcggcgg
    40321 taatatcgtt atcggaatcg ccataaaaaa ttcggatatt tttatcctgt agccattgcg
    40381 attttgtatt ttgccctggt ttatcgcccg caaagatcac cggattcatg ttggtggcag
    40441 gaatatgaaa attatccgcc agcgtttttg aaaccgtttc tgttttggtc gggctacgac
    40501 cagtcacaaa gaagatcgcg tcaccgcggc gtacatgcat atcaatcagc tggcgagcga
    40561 cctcttttgg aatgctgaat tcatcccagc cattgttcat tttttcccag aacacaggat
    40621 ttttcagata atcttcgctt tctggtgaga aggttttttt gccgcgccag aaacccggac
    40681 tggaaaaaag taccgtatca tcgatatcaa accccaccgc cattggcgga cgtcctgcga
    40741 ggctattttc aatttgtgca accgaaaccc aatgaattgg tgcctgttca gcgagtttgg
    40801 ctacgttagt acccggatta agcggtgaag gagatgaggc cagggcaaca gcggaactgt
    40861 ttagagcgaa caataagcaa acggcactca atgcctgtgt gatcttgcgc atatttttcc
    40921 ctaaatagtc agtaagttga aacttattat tgtgcgtttg ttgaaaaaac catctgacca
    40981 taacgccaac cgcaggggaa aggaaggatt atctgcggat ttgtcacata aaaagagcat
    41041 gatcacattt ggaatacctg tgcgataaac cacacattgc cacggttatt ctgcttacac
    41101 attcacacaa tggcactctt ttggttctcg caaacattgt tcagggaata tactgatgat
    41161 aaatataaat gcattcaatg gatgatgaat gaaaatatcg tggaattata tatttaagaa
    41221 caaatggcga tttcacatta caagcatttc actttttctt atcatgctcg cggtttcaat
    41281 cgcttttttg cacttgcgtt ttaatacctt gtccggtacc gataaaatgc ggcttgaaat
    41341 gtataagtcc acattatatt ccaccatcga gcaattttat gttttaccct atatgctctc
    41401 aacagaccat atcatccgtc aggcggtaat tacgcctgac gatatgacgt ccagcgaact
    41461 caatcaacga attgcacatt tcaatactca actcaaaacc gcagcaatat ttattctgga
    41521 tacccaaggt aaggccatcg cttctagtaa ctggcaggac cccggcagct atgtagggca
    41581 aaattatagc tatcgcccct attataaaca cgccatgtct ggcttaaatg gacgctttta
    41641 cggtattggt agcactacaa atacaccggg attcttcctc tctacaagta taaaagataa
    41701 aggaaaaatt gtcggtgttg tagtagtaaa aataagtctt aatgaaattg aaaaagcatg
    41761 ggccgaaggt cctgaaaata ttatcgtgaa tgatgaacat gggattatat ttttaagttc
    41821 aaaatcgcca tggcgaatgc gaacactgca accgttacct gttcaggcaa aacaaaaact
    41881 acaatctacc cgccaatata gtctcgacaa tcttttaccg gcggattatt atccctgtta
    41941 taccgtgagc aattttactt tcctgaaaga taaaaaagaa caactctgtt tattcccgca
    42001 acattatacg caacaaatag ccattccaga atttaactgg aaaatgacaa ttat
//
LOCUS       DAHHTG010000027        41584 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.027, whole genome shotgun sequence.
ACCESSION   DAHHTG010000027 DAHHTG010000000
VERSION     DAHHTG010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 41584)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41584)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41584
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.027"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            complement(<1..3880)
                     /gene="entF"
                     /locus_tag="NBI74_001442"
     CDS             complement(<1..3880)
                     /gene="entF"
                     /locus_tag="NBI74_001442"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin non-ribosomal peptide synthetase
                     EntF"
                     /protein_id="HCD1931803.1"
                     /translation="MSQHLPLVAAQPGIWMAEKLSDLPSAWSVAHYVELTGEVDAPLL
                     ARAVVAGLAQADTLRMRFMEDNGEVWQWVDDAQTFELPEIIDLRTNIDPHGTARALMQ
                     ADLQQDLRVDSGKPLVFHQLIQVADNRWYWYQRYHHLLVDGFSFPAITRQIANIYCAL
                     LRGEPTPASPFTPFADVVEEYQQYRESEAWQRDAAFWAEQRRQLPPPASLSPAPLPGR
                     SASADILRLKLEFTDAEFRQLATQLSGVQRTDLALALAALWLGRLCNRMDYAAGFIFM
                     RRLGSAALTATGPVLNVLPLGIHIAAQETLPQLATRLAAQLKKMRRHQRYDAEQIVRD
                     SGRAAGEEPLFGPVLNIKVFDYQLEIPGVQAQTHTLATGPVNDLELALFPDEHGDLSI
                     EILANKQRYDEPTLIQHAERLKMLIAQFAADPALLCGDVDIMLPGEYAQLAQINATQI
                     EIPETTLSALVAEQAAKTPDAPALADARYQFSYREMREQVVALANLLRERGVKPGDSV
                     AVALPRSVFLTLALHAIVEAGAAWLPLDTGYPDDRLKMMLEDARPSLLITTDDQLPRF
                     ADVPDLTRLCYNAPLTPQGSAPLKLSQPHHTAYIIFTSGSTGRPKGVMVGQTAIVNRL
                     LWMQNHYPLTGEDVVAQKTPCSFDVSVWEFFWPFIAGAKLVMAEPEAHRDPLAMQQFF
                     AEYGVTTTHFVPSMLAAFVASLTPQTARQNCATLKQVFCSGEALPADLCREWQQLTGA
                     PLHNLYGPTEAAVDVSWYPAFGEELAEVRGSSVPIGYPVWNTGLRILDAMMHPVPPGV
                     AGDLYLTGIQLAQGYLGRPDLTASRFIADPFAPGERMYRTGDVARWLDNGAVEYLGRS
                     DDQLKIRGQRIELGEIDRVMQALPDVEQAVTHACVINQAAATGGDARQLVGYLVSQSG
                     LPLDTSALQAQLRETLPPHMVPVVLLQLPQLPLSANGKLDRKALPLPELKAQTPGRVP
                     KAGSETIIAAAFSSLLGCDVQDADADFFALGGHSLLAMKLAAQLSRQFARQVTPGQVM
                     VASTVAKLATIIDGEEDSTRRMGFETILPLREGNGPTLFCFHPASGFAWQFSVLSRYL
                     DPQWSIIGIQSPRPHGPMQTAANLDEVCEAHLATLFEQQPHGPYYLLGYSLGGTLAQG
                     IAARLRARGEQVAFLGLLDTWPPETQNWQEKEANGLDPEVLAEINREREAFLAAQQGS
                     TSTELFTTIEGNYADAVRLLTTAHSVPFDAKATLFVAERTLQEGMSPEQAWAPWIAEL
                     DIYRQDCAHVDIISPVAFEKIGPIIRATLNK"
     gene            complement(3877..4095)
                     /gene="ybdZ"
                     /locus_tag="NBI74_001443"
     CDS             complement(3877..4095)
                     /gene="ybdZ"
                     /locus_tag="NBI74_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin biosynthesis protein YbdZ"
                     /protein_id="HCD1931804.1"
                     /translation="MAFSNPFDDPQGAFYILRNAQGQFSLWPQQCALPAGWDVVCQPQ
                     SQASCQQWLEAHWRTLTPANFTQLQEAQ"
     gene            complement(4098..5300)
                     /gene="fes"
                     /locus_tag="NBI74_001444"
     CDS             complement(4098..5300)
                     /gene="fes"
                     /locus_tag="NBI74_001444"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterochelin esterase"
                     /protein_id="HCD1931805.1"
                     /translation="MTALKVGSESWWQSKHGPEWQRLNDEMFEVTFWWRDPQGSEGHS
                     TIKRVWVYITGMTDHHQNSQPRSMQRIAGTDVWQWTTQLNANWRGSYCFIPTERDDIF
                     SAPSPDRLELREGWRKLLPQAIADPLNPQSWKGGRGHAVSALEMPQAPLQPGWDCPQA
                     PETPAKEIIWKSERLKNSRRVWIFTTGDATAEERPLAVLLDGEFWAQSMPVWPALTSL
                     THRRQLPPAVYVLIDAIDTTHRAHELPCNADFWLAVQQELLPQVKAIAPFSDRADRTV
                     VAGQSFGGLSALYAGLHWPERFGCVLSQSGSYWWPHRGGHQEGMLLEQLKTGEVSAEG
                     LRIVLEAGVREPMIMQANQALYAQLHPLKESIFWRQVDGGHDALCWRGGLMQGLIDLW
                     QPLFHDRS"
     gene            5338..5538
                     /locus_tag="NBI74_001445"
                     /pseudo
     CDS             5338..5538
                     /locus_tag="NBI74_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001174912.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            5543..7783
                     /gene="fepA"
                     /locus_tag="NBI74_001446"
     CDS             5543..7783
                     /gene="fepA"
                     /locus_tag="NBI74_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore enterobactin receptor FepA"
                     /protein_id="HCD1931806.1"
                     /translation="MNKKIHSLALLVNLGIYGVAQAQEPTDTPVSHDDTIVVTAAEQN
                     LQAPGVSTITADEIRKNPVARDVSEIIRTMPGVNLTGNSTSGQRGNNRQIDIRGMGPE
                     NTLILIDGKPVSSRNSVRQGWRGERDTRGDTSWVPPEMIERIEVLRGPAAARYGNGAA
                     GGVVNIITKKGSGEWHGSWDAYFNAPEHKEEGATKRTNFSLTGPLGDEFSFRLYGNLD
                     KTQADAWDINQGHQSARAGTYATTLPAGREGVINKDINGVVRWDFAPLQSLELEAGYS
                     RQGNLYAGDTQNTNSDAYTRSKYGDETNRLYRQNYSLTWNGGWDNGVTTSNWVQYEHT
                     RNSRIPEGLAGGTEGKFNEKAAQDFVDIDLDDVMLHSEVNLPIDFLVNQTLTLGTEWN
                     QQRMKDLSSNTQALTGTNTGGAIDGVSATDRSPYSKAEIFSLFAENNMELTDSTIVTP
                     GLRFDHHSIVGNNWSPALNISQGLGDDFTLKMGIARAYKAPSLYQTNPNYILYSKGQG
                     CYASAGGCYLQGNDDLKAETSINKEIGLEFKRDGWLAGVTWFRNDYRNKIEAGYVAVG
                     KNAVGTDLYQWDNVPKAVVEGLEGSLNVPVSETVMWTNNITYMLKSENKTTGDRLSII
                     PEYTLNSTLSWQAREDLSMQTTFTWYGKQQPKKYNYKGQPAVGPETKEISPYSIVGLS
                     ATWDVTKNVSLTGGVDNLFDKRLWRAGNAQTTGDLAGANYIAGAGAYTYNEPGRTWYM
                     SINTHF"
     gene            7958..8617
                     /gene="entD"
                     /locus_tag="NBI74_001447"
     CDS             7958..8617
                     /gene="entD"
                     /locus_tag="NBI74_001447"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009653162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin synthase subunit EntD"
                     /protein_id="HCD1931807.1"
                     /translation="MKTTHTSLPFAGHTLHFVEFDPASFREQDLLWLPHYAQLQHAGR
                     KRKTEHLAGRIAAIYALREYGYKCVPAIGELRQPVWPAGVYGSISHCGTTALAVVSRQ
                     PIGIDIEEIFSVQTARELTDNIITPAEHERLAECGLTFSLALTLAFSAKESAFKASEI
                     QTDAGFLDYQIGNCQHQQMNISGPNGDHIVQWLVRGNNVITLCLADKHPHTAACGLSG
                     G"
     gene            complement(8619..9554)
                     /locus_tag="NBI74_001448"
     CDS             complement(8619..9554)
                     /locus_tag="NBI74_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003831178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase family protein"
                     /protein_id="HCD1931808.1"
                     /translation="MRNSEHLANVMVQAFIDAVNEGIDLVPVVADSTSTAKIAPFIKQ
                     FPDRLVNVGIAEQSMVGTAAGLALGGKVAVTCNAAPFLIARANEQIKVDVCYNFTNVK
                     LFGLNAGASYGPLASTHHAIDDIAVMRGFGNIQIFAPSSPRECRQVIDYALRYQGPVY
                     IRMDGKALPEIHDEDYRFVPGAVVTLREGSELALVATGSTVHEVVDAAELLAQSGIAA
                     TVVSVPSIRPCDTKALLAALKGCKAVMTVEEHNVNGGLGSLVAEVLAEAGTGIKLKRA
                     GIMDGEYAAAADRGWLRQHHGFDAAGIAAQAREML"
     gene            complement(9547..10377)
                     /locus_tag="NBI74_001449"
     CDS             complement(9547..10377)
                     /locus_tag="NBI74_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001610581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HCD1931809.1"
                     /translation="MNPYSYDIQTLERKARAVRRHIVRLNANSPAGGHTGADLSQVEL
                     LTALYFRILNVAPDRLHDDDRDIYIQSKGHAVGCYYCVLAEAGFFPLEWLSTYQQPNS
                     HLPGHPVRQKTPGIELNTGALGHGFPVAVGLALAAKKSQSRRRIFLITGDGELAEGSN
                     WEAALAAAHYGLDNLVIINDKNNLQLAGPTREIMNTDPLAEKWLAFGMQVTECQGNDM
                     ASVVATLEELKQEGKPNVVIAHTTKGAGISFIQGRAEWHHRVPKGDEIEQALEELKDA
                     "
     gene            10739..11689
                     /locus_tag="NBI74_001450"
     CDS             10739..11689
                     /locus_tag="NBI74_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HCD1931810.1"
                     /translation="MAKQDEQRLLVKIATLYYLEGRKQSDIAQLLSLSQSFISRALTR
                     CQKEGVVKISVVQPSNIFLNLEKGIEERYGIKQAIVVDTEDDASDHTIKRAIGSAAAH
                     YLETRLRPKDFIGVSSWSSTIRAMVDEVHAQNLKASGVIQLLGGVGPNGNVQATILTQ
                     TLAQHLNCEAWLLPSQSIEGSVEEKKRLVASQDVADVIARFDDVDIAIVGIGILEPSQ
                     LLKTSGNYYHEDMLQVLADRGAVGDICLHYYDKHGQPVLQDDEDPVIGMALDKIKKCP
                     NVVALAGGKDKVAAIKGAMQGGYIDVLITDFPTARMLIAD"
     gene            12040..13560
                     /locus_tag="NBI74_001451"
     CDS             12040..13560
                     /locus_tag="NBI74_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000457341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HCD1931811.1"
                     /translation="MFAGNKEFVIALDEGTTNAKAIALDGFGNVVAKFSQPLAIQTPR
                     EGWVEQSGEALISASLDVIAQAINHVGAENVAALAISNQRETAIGWYRQSGKPLNAAI
                     TWQCTRSAEFCETLRREGKEQQIKTTTGLPIAPLFSASKMRWLLESTPEGVQLADQGE
                     ICLGTIDAWLLWNLTAGDSFCCDYSNAARTQLLNLKTGEWDEEMLALFHIPRAALPEI
                     KPSSGLFGYTRGLKTIPDGIPVMAMVGDSHAALFGHALGEAGCVKATYGTGSSVMAPV
                     NSAQCDIATLATTIAWHDGENIIYGLEGNIPHTGDAVAWMADSTGLSELTDAELALEL
                     NTLPASVDSTMGVYFVPALTGLGAPWGDDSARGVVCGLSRGVKRAHLIRAAMESITYQ
                     IADVVEAMQQHDDFHLSALMVDGGPTKNDWLMQYQADLLGCPVMRSDVPELSAIGAGL
                     LARKALIPGTVADLKALLTEHSEFKPDMARHQRLQKRWQEWRHAVNRTLWKPASSI"
     gene            13664..14599
                     /locus_tag="NBI74_001452"
     CDS             13664..14599
                     /locus_tag="NBI74_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose ABC transporter substrate-binding
                     protein"
                     /protein_id="HCD1931812.1"
                     /translation="MKLRLTLLTAATLTALSFSAHAVEKGTIMIMVNSLDNPYYASEA
                     KGASEKAQQLGYKTTILSHGEDVKKQNELIDTAIGKKVQGIILDNADSTASVAAIEKA
                     KKAGIPVILINREIPVDDVALVQITHNNFQAGSEVANAFVEKMGEKGKYAELTCNLAD
                     NNCVTRSKSFHQVIDQFPEMVSVAKQDAKGTLIDGKRIMDSILQAHPDVKGVICGNGP
                     VALGAIAALKAANRNDVIVVGIDGSNDERDAVKAESLQATVMLQAQAIAAQGVTDLDN
                     YLQKGVKPAKQRVMFRGILITKDNADKVQDFNIKS"
     gene            14646..15266
                     /locus_tag="NBI74_001453"
     CDS             14646..15266
                     /locus_tag="NBI74_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004111235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2291 domain-containing protein"
                     /protein_id="HCD1931813.1"
                     /translation="MDKKVWLALAILSLGGCRIVSQQELADLKSPPNPHMANIDQTWQ
                     KNIVPQVVENARPVAELMAALQAEKDVDAACKTLGYRSQEENPCIFYVKVEGGITNID
                     AKSRSGKMTITDISGTNIVVQTGPTLRGTLLRDAYKGASYEHFNDQVLFGEYGRAINE
                     QAINMIQSAKLKTGETVQVYGVFSAWDIPQTIPDITPAKIARAGGE"
     gene            15268..16812
                     /locus_tag="NBI74_001454"
     CDS             15268..16812
                     /locus_tag="NBI74_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HCD1931814.1"
                     /translation="MLPQEIAPPQTDESPVIIETHDLSRIYPGVVALDNVNYRVYRNK
                     VNVLIGENGAGKSTMMKMLAGVETPSSGKIILDGKEVTLNSTHQAEKHGISIIFQELN
                     LFPNMNVMDNIFMANEFFQKGKINEKYQYELAKSLLERLELNVDPYTQLGELGIGHQQ
                     LVEIARALSKDTRVLIMDEPTSALSQSEVKVLFNVIAQLKRRGVTIIYISHRLEELME
                     IGDFITIFRDGRFISERAVSDASIPWIIEQMVGDKKKHFDYQPATQGDTVLAVQGLTA
                     LHPSGGYKLNDVTFSLHKGEVIGIYGLLGAGRTELFKGLVGLMPCQSGKIQLNGETID
                     KCHFQARLKKGLALVPEDRQGEGVVQMMSIQSNMTLSDFSLQGFRRAWKWLNPQKESA
                     SVKEMILQLAIKVSDAELPITSLSGGNQQKVVLGKALMTQPQVVFLDEPTRGIDVGAK
                     TDVYQLVGKMAQQGLAVMFSSSELDEVMALADRILVMADGRITADLPRGEVTREKLIA
                     ASTPQD"
     gene            16830..17897
                     /locus_tag="NBI74_001455"
     CDS             16830..17897
                     /locus_tag="NBI74_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCD1931815.1"
                     /translation="MNQKYMIYMYLLKARTFIALLLVIAFFSVMVPNFLTASNLLIMT
                     QHVAITGLLAIGMTLVILTGGIDLSVGAVAGICGMVAGALLTNGLPLWSGNIIFFNVP
                     EVILCVAVFGILVGLVNGAVITRFGVAPFICTLGMMYVARGSALLFNDGSTYPNLNGM
                     ETLGNTGFATLGSGTFLGIYLPIWLMIGFLILGYWITTKTPLGRYIYAIGGNESAARL
                     AGVPIVKVKVFVYAFSGLCAAFVGLIVASQLQTAHPMTGNMFEMDAIGATVLGGTALA
                     GGRGRVSGSIIGAFVIVFLADGMVMMGVSDFWQMVIKGLVIVTAVVVDQFQQKLQNKV
                     ILMRRHEEKMAAAPAVKPLSS"
     gene            17951..18748
                     /locus_tag="NBI74_001456"
     CDS             17951..18748
                     /locus_tag="NBI74_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCD1931816.1"
                     /translation="MQRNFQGKTVVITGACRGIGAGIAERFARDGANLVMVSNAERVH
                     ETAEQLRQCYQADILSLEVDVTDEAQVQALYEQAAARFGSIDVSIQNAGIITIDYFDR
                     MPAADFEKVLAVNTTAVWLCCREAAKYMVKQNHGCLINTSSGQGRQGFIYTPHYAASK
                     MGVIGITQSLAHELAQWNITVNAFCPGIIESEMWDYNDRVWGEILSTDDKRYGKGELM
                     AEWVEGIPMKRAGKPEDVAGLVAFLASDDARYLTGQTINIDGGLIMS"
     gene            18781..19566
                     /locus_tag="NBI74_001457"
     CDS             18781..19566
                     /locus_tag="NBI74_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCD1931817.1"
                     /translation="MIAKDFAQQLFDLHDRVAFVTGAGSGIGQMIALALASAGSRVVC
                     FDLREDGGLKETVNNIEALGGQALYYTGDVRELRDLRAAVALAKTHFGRLDIAVNAAG
                     IANANPALEMESEQWQRVIDINLTGVWNSCKAEAELMVENGGGSIINIASMSGIIVNR
                     GLEQAHYNSSKAGVIHLSKSLAMEWVSKGIRVNSISPGYTATPMNTRPEMVHQTREFE
                     SQTPIQRMAKVEEMAGPALFLASDAASFCTGVDLVVDGGFVCW"
     gene            complement(19676..19828)
                     /gene="hokE"
                     /locus_tag="NBI74_001458"
     CDS             complement(19676..19828)
                     /gene="hokE"
                     /locus_tag="NBI74_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokE"
                     /protein_id="HCD1931818.1"
                     /translation="MLTKYALAAVIVLCLTVLGFTLLVGDSLCEFTVKERNIEFKAVL
                     AYEPKK"
     gene            complement(20178..20330)
                     /gene="hokE"
                     /locus_tag="NBI74_001459"
     CDS             complement(20178..20330)
                     /gene="hokE"
                     /locus_tag="NBI74_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokE"
                     /protein_id="HCD1931819.1"
                     /translation="MLTKYALVAVIVLCLTVLGFTLLVGDSLCEFTVKERNIEFKAVL
                     AYEPKK"
     gene            20773..21891
                     /locus_tag="NBI74_001460"
     CDS             20773..21891
                     /locus_tag="NBI74_001460"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HCD1931820.1"
                     /translation="MPLPDFHVSEPFTLGIELEMQVVNPPGYDLSQDSSMLIDAVKNQ
                     ITAGEVKHDITESMLELATDVCRDINQAAGQFSAMQKVVLQAAADHHLEICGGGTHPF
                     QKWQRQEVCDNERYQRTLQNFGYLIQQATVFGQHVHVGCASGDDAIYLLHGLSRFVPH
                     FIALSAASPYMQGTDTRFASSRPNIFSAFPDNGPMPWVSNWQQFEALFRCLSYTTMID
                     SIKDLHWDIRPSPHFGTVEVRVMDTPLTLSHAVNMAGLIQATAHWLLTERPFKHQEKD
                     YLLYKFNRFQACRYGLEGVITDPHTGDRRSLTEATLRLLEKIAPSAHKIGASSAIEAL
                     HRQVVSGLNEAQLMRDFVADGGSLIGLVKKHCEIWAGE"
     gene            21957..22205
                     /locus_tag="NBI74_001461"
     CDS             21957..22205
                     /locus_tag="NBI74_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1158 domain-containing protein"
                     /protein_id="HCD1931821.1"
                     /translation="MKHSLETLTTAAGILLMAFLSCLLLPAPALGLTLAQKLVTTFHL
                     MDLSQLYTLLFCLWFLVLGAIEYFFLRFICRRWFSLAD"
     gene            22270..22653
                     /locus_tag="NBI74_001462"
     CDS             22270..22653
                     /locus_tag="NBI74_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000360941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HCD1931822.1"
                     /translation="MDKQSLHETAKRLALELPFVELCWPFGPEFDVFKIGGKIFMLSS
                     ELRGVPFINLKSDPQKSLLNQQIYPSIKPGYHMNKKHWISVYPGEEISEALLRDLIND
                     SWNLVVDGLAKRDQKRLRPDSTVNQ"
     gene            22732..23385
                     /gene="nfsB"
                     /locus_tag="NBI74_001463"
     CDS             22732..23385
                     /gene="nfsB"
                     /locus_tag="NBI74_001463"
                     /EC_number="1.5.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-insensitive NAD(P)H nitroreductase"
                     /protein_id="HCD1931823.1"
                     /translation="MDIISVALKRHSTKAFDASKKLTPEQAEQIKTLLQYSPSSTNSQ
                     PWHFIVASTEEGKARVAKSAADNYVFNERKMLDASHVVVFCAKTAMDDAWLKLVVDQE
                     DADGRFATPEAKAANDKGRKFFADMHRKDLHDDAEWMAKQVYLNVGNFLLGVAALGLD
                     AVPIEGFDAAILDEEFGLKEKGYTSLVVVPVGHHSVEDFNATLPKSRLPQNITLTEV"
     gene            23493..24740
                     /gene="ybdG"
                     /locus_tag="NBI74_001464"
     CDS             23493..24740
                     /gene="ybdG"
                     /locus_tag="NBI74_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel YbdG"
                     /protein_id="HCD1931824.1"
                     /translation="MQDLISQVEDLAGIEIDHTTSMVMIFGIIFLTAVVVHIILHWVV
                     LRTFEKRAIASSRLWLQIITQNKLFHRLAFTLQGIIVNIQAVFWLQKGTEAADILTTC
                     AQLWIMMYALLSVFSLLDVILNLAQKFPAASQLPLKGIFQGIKLIGAILVGILMISLL
                     IDQSPAILISGLGAMAAVLMLVFKDPILGLVAGIQLSANDMLKLGDWLEMPKYGADGA
                     VIDIGLTTVKVRNWDNTITTIPTWSLVSDSFKNWSGMSASGGRRIKRSISIDVTSIRF
                     LDEDEMQRLNKAHLLKPYLTSRHQEINEWNRQQGSTESVLNLRRMTNIGTFRAYLNEY
                     LRNHPRIRKDMTLMVRQLAPGDNGLPLEIYAFTNTVVWLEYESIQADIFDHIFAIVEE
                     FGLRLHQSPTGNDIRSLAGAFKQ"
     gene            complement(24898..26280)
                     /gene="pheP"
                     /locus_tag="NBI74_001465"
     CDS             complement(24898..26280)
                     /gene="pheP"
                     /locus_tag="NBI74_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine transporter"
                     /protein_id="HCD1931825.1"
                     /translation="MKNASTVSEDTASNQEPTLHRGLHNRHIQLIALGGAIGTGLFLG
                     IGPAIQMAGPAVLLGYGVAGIIAFLIMRQLGEMVVEEPVSGSFAHFAYKYWGPFAGFL
                     SGWNYWVMFVLVGMAELTAAGIYMQYWFPDVPTWIWAAAFFIIINAVNLVNVRLYGET
                     EFWFALIKVLAIIGMIGFGLWLLFSGHGGEKASIDNLWRYGGFFATGWNGLILSLAVI
                     MFSFGGLELIGITAAEARDPEKSIPKAVNQVVYRILLFYIGSLVVLLALYPWVEVKSN
                     SSPFVMIFHNLDSNVVASALNFVILVASLSVYNSGVYSNSRMLFGLSVQGNAPKFLTR
                     VSRRGVPINSLMLSGAITSLVVLINYLLPQKAFGLLMALVVATLLLNWIMICLAHLRF
                     RAAMRRQGRETQFKALLYPFGNYLCIAFLGMILLLMCTMDDMRLSAILLPVWIVFLFV
                     AFKTLRRKVN"
     gene            complement(26382..29525)
                     /gene="cusA"
                     /locus_tag="NBI74_001466"
     CDS             complement(26382..29525)
                     /gene="cusA"
                     /locus_tag="NBI74_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter permease
                     subunit CusA"
                     /protein_id="HCD1931826.1"
                     /translation="MIEWIIRRSVANRFLVLMGALFLSIWGTWTIINTPVDALPDLSD
                     VQVIIKTSYPGQAPQIVENQVTYPLTTTMLSVPGAKTVRGFSQFGDSYVYVIFEDGTD
                     PYWARSRVLEYLNQVQGKLPAGVSAELGPDATGVGWIYEYALVDRSGKHDLADLRSLQ
                     DWFLKYELKTIPDVAEVASVGGVVKEYQVVIDPQRLAQYGISLADVKSALDASNQEAG
                     GSSIELAEAEYMVRASGYLQTLDDFNHIVLKASENGVPVYLRDVAKVQVGPEMRRGIA
                     ELNGEGEVAGGVVILRSGKNAREVIAAVKDKLETLKSSLPEGVEIVTTYDRSQLIDRA
                     IDNLSGKLLEEFIVVAVVCALFLWHVRSALVAIISLPLGLCIAFIVMHFQGLNANIMS
                     LGGIAIAVGAMVDAAIVMIENAHKRLEEWLHQHPDATLDNKTRWQVITDASVEVGPAL
                     FISLLIITLSFIPIFTLEGQEGRLFGPLAFTKTYAMAGAALLAIVVIPILMGYWIRGK
                     IPPESSNPLNRFLIRVYHPLLLKVLHWPKTTLLVAALSVLTVLWPLNKVGGEFLPQIN
                     EGDLLYMPSTLPGISAAEAASMLQKTDKLIMSVPEVARVFGKTGKAETATDSAPLEMV
                     ETTIQLKPQDQWRPGMTMDKIIEELDNTVRLPGLANLWVPPIRNRIDMLSTGIKSPIG
                     IKVSGTVLADIDTMAEQIEEVARTVPGVASALAERLEGGRYINVEINREKAARYGMTV
                     ADVQLFVTSAVGGAMVGETVEGIARYPINLRYPQSWRDSPQALRQLPILTPMKQQITL
                     ADVADVKVSTGPSMLKTENARPTSWIYIDARDRDMVSVVHDLQKAIAEKVQLKPGTSV
                     AFSGQFELLERANHKLKLMVPMTLMIIFVLLYLAFRRVGEALLIISSVPFALVGGIWL
                     LWWMGFHLSVATGTGFIALAGVAAEFGVVMLMYLRHAIEAEPSLNNPQTFSEQKLDEA
                     LYHGAVLRVRPKAMTVAVIIAGLLPILWGTGAGSEVMSRIAAPMIGGMITAPLLSLFI
                     IPAAYKLMWLHRHRVRK"
     gene            complement(29537..30760)
                     /gene="cusB"
                     /locus_tag="NBI74_001467"
     CDS             complement(29537..30760)
                     /gene="cusB"
                     /locus_tag="NBI74_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     adaptor subunit CusB"
                     /protein_id="HCD1931827.1"
                     /translation="MKKIALIIGSMIAGGIISAAGFTWFAKEEPPAEKTSTAERKVLF
                     WYDPMYPNTRFDKPGKSPFMDMDLVPKYADEESSASGVRIDPTQTQNLGVKTATVTRG
                     PLTFAQSFPANVSYNEYQYAIVQARAAGFIDKVYPLTVGDKVQKGAPLLDLTIPDWVE
                     AQSEYLLLRETGGTATQTEGILERLRLAGMPEADIRRLIATQKIQTRFTLKAPIDGVI
                     TAFDLRAGMNIAKDNVVAKIQGMDPVWVTAAIPESIAWLVKDASQFTLTVPARPDKTL
                     TIRKWTLLPGVDAATRTLQLRLEVDNADEALKPGMNAGLQLNTASEPMLLIPSQALID
                     TGNEQRVITVDADGRFVPKRVAVFQASQGVTALRSGLAEGEKVVSSGLFLIDSEANIS
                     GALERMRSESATHAH"
     gene            complement(30776..31108)
                     /gene="cusF"
                     /locus_tag="NBI74_001468"
     CDS             complement(30776..31108)
                     /gene="cusF"
                     /locus_tag="NBI74_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     metallochaperone CusF"
                     /protein_id="HCD1931828.1"
                     /translation="MKKALQVAMFSLFTVIGFNAQANEHHHETMSEAQPQVISATGVV
                     KGVDLESKKITIHHDPIAAVNWPEMTMRFTITPQTKMSEIKTGDKVAFNFVQQGNLSL
                     LQDIKVSQ"
     gene            complement(31132..32514)
                     /gene="cusC"
                     /locus_tag="NBI74_001469"
     CDS             complement(31132..32514)
                     /gene="cusC"
                     /locus_tag="NBI74_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter outer
                     membrane channel CusC"
                     /protein_id="HCD1931829.1"
                     /translation="MSPCKLLPFCVALALTGCSLAPDYQRPAMPVPQQFSLSQNGLVN
                     AADNYQNAGWRTFFVDNQVKTLISEALVNNRDLRMAALKVQEARAQYRLTDADRYPQL
                     NGEGSGSWSGNLKGDSATTREFSTGLNASFDLDFFGRLKNMSEAERQNYLATEEAQRA
                     VHILLVSNVAQSYFNQQLAYAQLQIAEETLRNYQQSYAFVEKQLLTGSSNVLALEQAR
                     GVIESTRSDIAKRQGELAQANNALQLLLGSYGKLPQAQTVNSDSLQSVKLPAGLSSQI
                     LLQRPDIMEAEHALMAANANIGAARAAFFPSISLTSGISTASSDLSSLFNASSGMWNF
                     IPKIEIPIFNAGRNQANLDIAEIRQQQSVVNYEQKIQNAFKEVADALALRQSLNDQIS
                     AQQRYLASLQITLQRARALYQHGAVSYLEVLDAERSLFATRQTLLDLNYARQVNEISL
                     YTALGGGWQQ"
     gene            32671..33354
                     /gene="cusR"
                     /locus_tag="NBI74_001470"
     CDS             32671..33354
                     /gene="cusR"
                     /locus_tag="NBI74_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper response regulator transcription factor
                     CusR"
                     /protein_id="HCD1931830.1"
                     /translation="MKLLIVEDEKKTGEYLTKGLTEAGFVVDLADNGLNGYHLAMTGD
                     YDLIILDIMLPDVNGWDIVRMLRSANKGMPILLLTALGTIEHRVKGLELGADDYLVKP
                     FAFAELLARVRTLLRRGAAVIIESQFQVADLMVDLVSRKVTRSGTRITLTSKEFTLLE
                     FFLRHQGEVLPRSLIASQVWDMNFDSDTNAIDVAVKRLRGKIDNDFEPKLIQTVRGVG
                     YMLEVPDGQ"
     gene            33344..34786
                     /gene="cusS"
                     /locus_tag="NBI74_001471"
     CDS             33344..34786
                     /gene="cusS"
                     /locus_tag="NBI74_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) sensor histidine kinase CusS"
                     /protein_id="HCD1931831.1"
                     /translation="MVSKPFQRPFSLATRLTFFISLATIAAFFAFAWIMIHSVKVHFA
                     EQDINDLKEISATLERVLNHPDETQARRLMTLEDIVSGYSNVLISLANSHGKTVYHSP
                     GAPDIREFTRDAIPDKDAQAGEVYLLSGPTMMMPGHGHGHMEHSNWRMINLPVGPLVD
                     GKPIYTLYIALSIDFHLHYINDLMNKLIMTASVISILIVFIVLLAVHKGHAPIRSVSR
                     QIQNITSKDLDVRLDPQTVPIELEQLVLSFNHMIERIEDVFTRQSNFSADIAHEIRTP
                     ITNLITQTEIALSQSRSQKELEDVLYSNLEELTRMAKMVSDMLFLAQADNNQLIPEKK
                     ILNLADEVGKVFDFFEALAEDRGVELRFVGDECQVAGDPLMLRRALSNLLSNALRYTP
                     TGETIVVGCQMVDHLVQVTVENPGTPIAPEHLPRLFDRFYRVDPSRQRKGEGSGIGLA
                     IVKSIVVAHKGTVAVTSDARGTRFVIILPA"
     gene            34936..37173
                     /gene="nrfB"
                     /locus_tag="NBI74_001472"
     CDS             34936..37173
                     /gene="nrfB"
                     /locus_tag="NBI74_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage adsorption protein NrfB"
                     /protein_id="HCD1931832.1"
                     /translation="MDWLLDVFATWLYGLKVIAITLAVIMFISGLDDFFIDVVYWVRR
                     IKRKLSVYRRYPRMSYRELYKPDEKPLAIMVPAWNETGVIGNMAELAATTLDYENYHI
                     FVGTYPNDPDTQRDVDEVCARFPNVHKVVCARPGPTSKADCLNNVLDAITQFERSANF
                     AFAGFILHDAEDVISPMELRLFNYLVERKDLIQIPVYPFEREWTHFTSMTYIDEFSEL
                     HGKDVPVREALAGQVPSAGVGTCFSRRAVTALLADGDGIAFDVQSLTEDYDIGFRLKE
                     KGMTEIFVRFPVVDEAKEREQRKFLQHARTSNMICVREYFPDTFSTAVRQKSRWIIGI
                     VFQGFKTHKWTSSLTLNYFLWRDRKGAISNFVSFLAMLVMIQLLLLLAYESLWPDAWH
                     FLSIFSGSAWLMTLLWLNFGLMINRIVQRVIFVTGYYGLTQGLLSVLRLFWGNLINFM
                     ANWRALKQVLQHGDPRRVAWDKTTHDFPSVTGDTRSLRPLGQILLENQVITEEQLDTA
                     LRNRVEGLRLGGSMLMQGLISAEQLAQALAEQNGVAWESIDAWQIPSSLIAEMPASVA
                     LHYAVLPLRLDNDELIVGSEDGIDPVSLAALTRKVGRKVRYVIVLRGQIVTGLRHWYA
                     RRRGHDPRAMLYNAVQHQWLTEQQAGEIWRQYVPHQFLFAEILTTLGHINRSAINVLL
                     LRHERSSLPLGKFLVTEGVISQETLDRVLTIQRELQVSMQSLLLKAGLNTEQVAQLES
                     ENEGE"
     gene            37160..40132
                     /gene="nfrA"
                     /locus_tag="NBI74_001473"
     CDS             37160..40132
                     /gene="nfrA"
                     /locus_tag="NBI74_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage adsorption protein NfrA"
                     /protein_id="HCD1931833.1"
                     /translation="MKENNLNRVIGWSGLLLTSLLSTSALADNIGTSAEELGLSDYRH
                     FVIYPRLDKALKAQKNNDEATAIREFEYIHQQVPDNIPLTLYLAEAYRHFGHDDRARL
                     LLEDQLKRHPGDDRLEHSLAAIPVEVKSVTTVEELLAQQKACDAAPTLRCRSEVGQNA
                     LRLAQLPVARAQLNDATFAASPEGKTLRTDLLQRAIYLKQWSQADTLYNEARQQNTLS
                     AAERRQWFDVLLAAQLDDRILALQSQGIFTDPQSYITYATALAYRGEKARLQHYLIEN
                     KPLFTTDAQEKSWLYLLSKYSANPVQALANYTVQFADNRQYVVGATLPVLLKEGQYDA
                     AQKLLATLPANEMLEERYAVSVATRNKAEALRLARLLYQQEPANLTRLDQLTWQLMQN
                     GQSREAADLLLQRYPFQGDARVSQTLMARLASLLESHPYLATPAKVAILSKPLPLAEQ
                     RQWQSQLPGIADNCPAIVRLLGDMSPSYDAAAWNRLAKCYRDTLPGVALYAWLQAEQR
                     QPNAWQHRAVAYQAYQVEDYATALTAWQKISLHDMSNEDLLAAANTAQAAGNGAARDR
                     WLQQAEQRGLGNNALYWWLHAQRYIPGQPELALNDLTRSINIAPSANAYVARATIYRQ
                     RHNIPAAVSDLRAALELEPNNSNTQAALGYALWDSGDIAQSREMLEQAHKGLPDDPAL
                     IRQLAYVNQRLDDMPATQHYARLVIDDIDNQALITPLTPEQNQQRFNFRRLHEEVGRR
                     WTFSFDSSIGLRSGAMSTANNNVGGAAPGKSYRSYGQLEAEYRLGRNMLLEGDLLSVY
                     SRVFADTGENGVMMPVKNPMSGTGLRWKPLRDQIFFLAVEQQLPLNGQNGASDTMLRA
                     SASFFNGGKYSDEWHPNGSGWFAQNLYLDAAQYIRQDIQAWTADYRVSWHQKVANGQT
                     IEPYAHVQDNGYRDKGTQGAQLGGVGVRWNIWTGETHYDAWPHKVSLGVEYQHTFKAI
                     NQRNGERNNAFLTIGVHW"
     gene            40133..41023
                     /locus_tag="NBI74_001474"
     CDS             40133..41023
                     /locus_tag="NBI74_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4434 family protein"
                     /protein_id="HCD1931834.1"
                     /translation="MRKFIFVLLTLLLVSPFSFAMKGIIWQPQNRDSQVTDTQWQGLM
                     SQLRLQGFDTLVLQWTRYGDAFTQPEQRALLFKRATAAQQAGLKLIVGLNADPEFFMH
                     QKQSSAALESYLNRLLAADLQQARLWSAVPGVTPDGWYISAEIDDLNWRSEAARQPLL
                     TWLNNAQRLISDVSAKPVYISSFFAGNMSPDGYRQLLEQVKATGVNVWVQDGSGVDKL
                     TAEQRERYLQASADCQSSAPASGIVYELFVAGKGKTFTAKPKPDAEIASLLAKRSSCG
                     KDTLYFSLRYLPVAQSILEY"
BASE COUNT        10229 a        10913 c        10595 g         9847 t
ORIGIN      
        1 acttatttag cgtcgcacga ataatcggcc caattttttc aaacgccacc ggagagataa
       61 tatccacatg cgcacaatcc tgacgataga tatccagctc agcaatccac ggtgcccagg
      121 cctgttcagg actcatacct tcctgaagcg tgcgttcggc aacaaatagc gtcgctttag
      181 catcaaacgg tacgctatga gctgtcgtca gcaggcgcac agcatcagcg tagttgcctt
      241 caatggtggt aaacaactcc gttgaagtac ttccctgctg tgccgccagg aaggcttcgc
      301 gctcgcggtt aatctccgcc agcacttccg ggtccagacc attggcttct ttttcctgcc
      361 agttttgcgt ttctggcggc caggtatcca gcaagccaag aaatgccacc tgttcgccac
      421 gggcacgcag tcgcgccgca atgccctgcg ccagcgtacc gccaagtgaa taacccagca
      481 ggtaataagg gccgtgcggt tgttgttcaa ataacgttgc cagatgcgct tcgcagactt
      541 catccaggtt tgccgccgtc tgcatggggc catgagggcg cggcgactga atgccgataa
      601 tcgaccattg tggatcgaga taacgcgaga gcacgctgaa ctgccaggca aaaccggatg
      661 ccggatggaa acaaaacagc gtcgggccat taccttcacg caacggcaga atggtttcga
      721 atcccatgcg tcgggtgctg tcttcttcac catcaataat cgttgccagt ttggcgacag
      781 ttgacgcgac catcacctgc cccggcgtca cctgacgggc aaactgtcga cttaactgcg
      841 ctgccagttt cattgccagt agcgaatgac cgccaagcgc aaagaaatca gcatcggcat
      901 cctgcacgtc acaacccagc aacgacgaga atgccgcggc gataatcgtt tcactgcccg
      961 ctttcggcac acgccccggc gtttgtgcct tcagttcagg caacggtaag gctttgcgat
     1021 ccagcttgcc gttggcgcta agtggtaact gtggaagttg cagcagaacc accggtacca
     1081 tatgtggtgg caatgtttct cgaagctgcg cctgtaatgc gctggtatcc aacggcaggc
     1141 ccgattgcga caccagatag cccaccaact gacgcgcatc accaccagtg gctgccgcct
     1201 ggttaatcac acaggcgtgg gtaacggctt gttcgacatc cggcagcgcc tgcatcacgc
     1261 gatcgatttc gcccagttca atacgctgcc cgcgaatttt tagctgatcg tcactgcgcc
     1321 ctaggtattc cactgcgccg ttatccagcc agcgggcaac gtctccggta cggtacatcc
     1381 gttcacctgg ggcaaaagga tcggcaataa agcggctggc ggtcagatcg ggtcgtccaa
     1441 gatacccctg cgccagttga ataccggtga gatagagatc gcccgccaca cccggcggca
     1501 ccggatgcat catcgcatcg agaatgcgca ggcccgtatt ccacaccgga taaccaatcg
     1561 gcacactgct gccgcgcacc tctgccagtt cctcgccaaa agccggatac caactgacat
     1621 ctaccgccgc ttccgtcggg ccatatagat tatgcaacgg cgcgccggtt aactgttgcc
     1681 attcgcggca taaatcagcc ggtagggctt caccactaca gaaaacctgt ttcaacgtcg
     1741 cgcaattctg acgagcggtt tgcggcgtca gcgatgcaac aaatgctgcc agcatcgacg
     1801 gcacaaagtg cgtggtcgtt acaccatatt cggcaaagaa ttgctgcata gcgagcgggt
     1861 cgcggtgcgc ttccggttca gccatcacca gtttagcccc ggcaataaac ggccagaaaa
     1921 actcccacac cgagacatca aaactgcatg gcgttttttg ggcaacgaca tcttcgcctg
     1981 taagcgggta atggttttgc atccaaagca ggcggttaac gatagccgtc tgcccgacca
     2041 ttaccccttt tggtctgccg gtggagccag aggtaaagat gatataagcc gtgtggtgcg
     2101 gttgtgaaag tttcagcggc gcactgccct gcggtgtaag cggggcgtta tagcaaaggc
     2161 gggttaaatc tggaacatcg gcaaagcgcg gcagttgatc gtcagtggtg attaacagcg
     2221 acggacgcgc atcttccagc atcattttca ggcgatcgtc cggatagccg gtatccagcg
     2281 gtaaccaggc cgcaccagcc tcaacaattg catgtagcgc gagagtcaaa aagaccgagc
     2341 gcggtagagc caccgccacg ctgtcgcccg gtttaacgcc gcgctcacgc agcagattcg
     2401 ccagcgccac cacctgctcg cgcatttccc gatagctgaa ctggtaacgc gcatctgcca
     2461 gcgccggagc atccggtgtt tttgccgctt gttctgccac cagcgcgctc agcgtggttt
     2521 ctggaatctc aatctgagtg gcgttgatct gcgccagctg cgcatactca cctggcagca
     2581 taatatcgac atcgccgcac aacagcgccg gatccgcggc gaactgggca atcagcattt
     2641 tcaggcgttc agcgtgttga attaacgttg gctcatcgta acgctgttta ttggcgagga
     2701 tttcaatact caaatctccg tgttcatccg ggaacagggc cagttcaagg tcattaaccg
     2761 gaccggttgc cagggtatgg gtttgcgcct gaacaccagg aatttccagt tggtaatcaa
     2821 ataccttgat attgagtacc ggaccgaaca gcggttcctc tcctgccgct cgcccactgt
     2881 cacggacaat ttgttcggcg tcgtagcgtt gatggcggcg catttttttc agttgtgctg
     2941 ccagtcgggt tgccagttgc ggcagcgttt cttgcgccgc aatgtgaata cccaacggta
     3001 aaacgttgag cacgggtccg gtagccgtca gcgccgccga gcccagtcga cgcataaaga
     3061 taaatccggc agcgtagtcc atgcggttgc acaatcgccc cagccacaag gctgccaacg
     3121 caagtgctaa atcggtacgc tgcacacctg aaagttgcgt agccagctgg cggaattccg
     3181 cgtcggtaaa ttccagtttc aggcgcaaaa tatctgccga agcgctgcgc cccggtaaag
     3241 gtgccggaga aagtgacgcg ggcggtggca gttgacgacg ctgttccgcc cagaatgccg
     3301 catcacgctg ccaggcttca ctttcgcggt actgctgata ctcttccact acatcagcga
     3361 aaggcgtaaa cggcgaagca ggcgttggtt cgccacgcag caatgcgcag taaatattgg
     3421 cgatctggcg tgtaatggcc gggaaactga agccatcgac cagcaaatgg tgataacgct
     3481 gataccagta ccagcggtta tccgccacct gtatcagctg atgaaagacc agtggtttac
     3541 cactatcgac gcgcagatct tgttgcaaat ccgcctgcat taatgcccgc gcagtaccgt
     3601 gcggatcaat gttggttcgc aggtcgataa tttctggcag ttcgaacgtc tgcgcatcat
     3661 cgacccactg ccagacttcg ccgttatctt ccataaaacg catccgcaac gtatctgctt
     3721 gcgctagtcc ggcaaccacc gcgcgggcca gtagtggcgc atcaacctct ccggttaact
     3781 caacgtaatg cgccacgctc caggcggagg gtaaatccga cagtttttct gccatccaga
     3841 tgccgggctg tgcggcgacc aaaggtaaat gctggctcat tgtgcctcct gcaactgggt
     3901 gaaattcgcc ggtgtcagag tacgccagtg ggcttccagc cactgctggc aggacgcctg
     3961 tgactgcggc tgacacacaa cgtcccagcc tgccggtaag gcgcattgtt gcggccacag
     4021 actgaattgc ccctgcgcat tgcgcaaaat gtaaaacgct ccctgtggat catcgaaggg
     4081 attactgaat gccatattca actcctgtca tggaaaagtg gttgccagag gtcgattagc
     4141 ccctgcatca agccaccgcg ccaacaaagc gcatcatgtc cgccgtcaac ctgacgccag
     4201 aaaatggatt cttttagggg gtgtaattgc gcatacagcg cctgattggc ctgcatgatc
     4261 atcggctcgc gaacacctgc ttccagcaca atgcgcagac cttcggcgct aacttcacca
     4321 gtttttagct gttcaagtaa catgccctct tggtgcccgc cccgatgcgg ccaccagtac
     4381 gaacctgact ggcttaatac acagccaaag cgttcaggcc agtgcagtcc ggcatacagc
     4441 gcggaaagcc caccaaaact ctgtccggcg accacggtgc gatcggcgcg atcgctaaaa
     4501 ggggcaatag ctttcacctg gggtaataac tcttgctgta ctgccagcca gaaatccgca
     4561 ttacacggca gttcgtgggc gcggtgcgtg gtgtcgatag cgtcgatcaa cacatacacg
     4621 gcgggcggaa gttgccgacg atgggtcagc gaagtcagcg caggccagac gggcatactc
     4681 tgcgcccaaa attcgccatc aagcaaaact gccagcgggc gttcttctgc tgttgcatcg
     4741 ccggtggtaa aaatccatac acggcgtgaa tttttcaacc gttcactttt ccagataatt
     4801 tctttggcag gcgtttctgg cgcttgcgga caatcccatc ccggttgcag aggcgcttgc
     4861 ggcatttcga gtgcagaaac agcgtgccct cgcccgcctt tccagctttg tggattcagc
     4921 ggatcggcta tcgcctgggg taatagtttt cgccagcctt cgcgtaattc gaggcgatcg
     4981 ggggatggtg cagaaaaaat gtcatcgcgt tcagtaggaa taaagcagta gctgccgcgc
     5041 cagttggcat tgagttgtgt cgtccactgc caaacatcgg ttcctgcaat tcgctgcatc
     5101 gaccggggct ggctgttctg atggtgatcg gtcatgccag tgatatagac ccatacgcgc
     5161 tttatcgtcg agtggccttc agaaccttgt ggatcacgcc accaaaaagt gacttcaaac
     5221 atttcgtcat tcagacgttg ccattccggg ccatgtttcg actgccacca gctctcactt
     5281 cctactttta acgccgtcac accataaccc catgtttact gtgcaatttt ttattgattg
     5341 cagaaatata ttgataatat tattgataac tatttgcatt tgcaatagcg taatggcgcg
     5401 ccgtgggaag cgcggacatt aattaatcag ctgcactgcg tgtctttcag gatcaaaggt
     5461 tttcgcggta gcgggatgcg tcgtgttgat gacgaccatg cccgacagtt gcaattcgtg
     5521 gcaaaaatgc aggaataaaa caatgaacaa gaagattcat tccctggcct tgttggtcaa
     5581 tctggggatt tatggggtag cacaggcaca agagccgacc gatactcctg tttcacatga
     5641 cgatactatt gttgttaccg ccgccgagca gaacttgcag gcgcccggcg tttcgaccat
     5701 taccgcagat gaaatccgca aaaacccggt tgcccgcgat gtatcggaga tcattcgtac
     5761 catgcctggc gttaacctga ccggtaattc caccagtggt cagcgtggta ataaccgcca
     5821 gattgatatt cgcggcatgg gtccggaaaa cacgctgatt ttgattgacg gcaagccggt
     5881 aagcagccgt aactcagtgc gtcagggctg gcgtggcgag cgcgataccc gtggtgatac
     5941 ctcctgggtg ccgcctgaaa tgattgaacg tattgaagtt ctacgtggtc cggcagctgc
     6001 gcgttatggc aacggcgcgg cgggcggcgt ggttaacatc attaccaaaa aaggcagcgg
     6061 tgagtggcac ggctcctggg atgcttattt caatgcgcca gaacataaag aggaaggtgc
     6121 caccaaacgc accaacttca gcctgaccgg tccgctgggc gacgaattca gcttccgctt
     6181 gtatggcaac ctcgacaaaa cccaggctga cgcgtgggat atcaaccagg gccatcagtc
     6241 cgcgcgtgcc ggaacgtatg ccacgacgtt accagccggg cgcgaagggg tgatcaataa
     6301 agatattaac ggcgtggtgc gctgggactt cgcgcctctg caatcactcg aactggaagc
     6361 gggctacagc cgccagggta acctgtatgc gggtgatacg cagaacacca actctgacgc
     6421 ttacactcgc tcgaaatatg gcgatgaaac caaccgcctg tatcgccaga actactcgct
     6481 gacctggaac ggcggctggg ataacggcgt gaccaccagc aactgggtgc agtacgaaca
     6541 cacccgtaac tcgcgtattc cggaaggtct ggcgggcggt accgaaggga aatttaacga
     6601 aaaagcggca caggattttg tcgatatcga tcttgatgac gtgatgctgc acagcgaagt
     6661 taacctgccg attgatttcc tcgtaaacca aacgctgacg ctgggtacag agtggaatca
     6721 gcaacggatg aaggacttga gttccaacac ccaggcactc accggaacga ataccggcgg
     6781 tgctattgat ggtgtgagtg ccaccgaccg tagcccatat tcaaaagcag aaattttctc
     6841 gctgtttgcc gaaaacaata tggagctgac tgacagcacc atcgtaacgc cggggctgcg
     6901 tttcgatcat cacagtattg tcggcaataa ctggagcccg gcgctgaaca tttcgcaagg
     6961 tttaggcgat gacttcacgc tgaaaatggg catcgcccgc gcctataaag cgccgagcct
     7021 gtaccagact aacccaaact acattctcta cagtaaaggt cagggctgct atgccagcgc
     7081 gggcggctgc tatctgcaag gtaacgatga cctgaaagca gaaaccagca tcaacaagga
     7141 gattggtctg gagttcaaac gcgacggttg gctggcgggc gtgacctggt tccgtaacga
     7201 ttatcgcaat aagattgaag cgggctatgt agctgtaggg aaaaacgcag tcggcaccga
     7261 tctctatcag tgggataacg tgccgaaagc ggtggttgaa ggtctggaag gatcgttaaa
     7321 cgtaccggtt agcgaaacgg tgatgtggac caataacatc acttatatgc tgaagagtga
     7381 aaacaaaacc acgggcgacc gtttgtcgat catcccggag tatacgttga actcaacgct
     7441 gagctggcag gcacgggaag atttgtcgat gcaaacgacc ttcacctggt acggcaagca
     7501 gcagccgaag aagtacaact acaaaggtca gccagcggtt ggaccggaaa ccaaagaaat
     7561 cagtccgtac agcattgttg gcctgagcgc gacctgggat gtgacgaaga atgtcagtct
     7621 gaccggcggc gtggacaacc tgttcgacaa acgtttgtgg cgtgcgggta atgcccagac
     7681 cacgggcgat ctggcaggag ccaactatat cgcaggtgcc ggggcgtata cctataacga
     7741 gccgggacgt acgtggtata tgagcattaa tactcacttc tgaagctaac gtcagattgt
     7801 tgacaaagtg cgcgtagttc atgccggatg cggcgtgaac gccttatccg gcctacaaaa
     7861 atcttgccaa ttcaatatat tgcaggacca cgtaggcctg ataagcgtag cgcatcaggc
     7921 agttttgcgt ttgtcatcat tctctaatat ggttgacatg aaaactacgc atacctccct
     7981 cccctttgcc ggacatacgc tgcattttgt tgagttcgat ccggcgagtt ttcgcgagca
     8041 ggatttactc tggctgccgc actacgcaca acttcaacac gctggacgta aacgtaaaac
     8101 agagcattta gccgggcgga tcgctgcaat ttatgcgctg cgggaatatg gctataaatg
     8161 tgtgcctgca atcggcgagc tacgccaacc tgtgtggcct gcgggggtat acggcagcat
     8221 tagtcactgt gggactacgg cattagccgt ggtatctcgt caaccgattg gcattgatat
     8281 cgaagagatt ttctctgtac aaaccgcaag agaattgaca gacaacatta ttacaccagc
     8341 agaacacgag cgactcgcag aatgcggttt aaccttttct ctggcgctga cactggcatt
     8401 ttccgccaaa gagagcgcat ttaaggcaag tgagatccaa actgatgcag gttttctgga
     8461 ctatcaaatt ggaaattgtc agcatcaaca gatgaatata agcgggccga atggcgacca
     8521 tatcgtgcag tggttagtaa ggggtaataa tgttattacc ttgtgcctgg cagataaaca
     8581 cccgcacact gcagcatgcg ggttatctgg cgggtaattc aaagcatttc ccgtgcctga
     8641 gctgcaattc cggcagcatc aaagccatga tgctgacgca accacccacg atccgccgct
     8701 gcggcatatt ctccatccat aatgcccgcg cgttttaatt tgatccccgt tccggcctct
     8761 gccaacactt ccgcgaccag gctaccaagg ccgccattga cgttatgctc ttcaaccgtc
     8821 atcactgctt tgcatccttt taacgctgcc agcaatgctt tggtgtcgca tgggcgaatc
     8881 gacggtacgc tgacaactgt agccgcgata ccagactgcg ctaaaagttc tgcggcatcg
     8941 accacttcat gaacggtaga gcctgtggct accagcgcca gttcgctgcc ctcgcgcaaa
     9001 gtcaccacgg cacctggtac aaatcggtag tcctcatcgt ggatttctgg cagggcttta
     9061 ccgtccatac ggatataaac aggcccctga tagcgaagag cataatcaat cacctgacgg
     9121 cactcccgcg gtgaagacgg ggcaaagatt tgaatattgc cgaaaccacg cataaccgca
     9181 atgtcatcaa tcgcatgatg cgtgctcgcc agcggtccat aactcgcccc ggcattcaga
     9241 ccgaacaatt ttacgttggt aaagttgtag cagacatcaa ccttgatttg ctcgttagcg
     9301 cgagcgataa gaaacggcgc ggcattgcag gtaacagcca ccttgccgcc cagcgccagc
     9361 ccggcagctg taccaaccat gctctgttcg gcaataccca cattcaccag tcggtcaggg
     9421 aattgcttaa taaagggagc aatttttgcc gtagacgttg aatcagccac cacaggcacc
     9481 aaatcgatcc cctcatttac ggcatcgata aacgcctgaa ccatcacatt tgccagatgt
     9541 tcactgttac gcatctttga gttcctccag tgcctgttca atttcatcgc ctttcggcac
     9601 ccggtgatgc cattccgcac gcccttgtat aaaggaaatg cctgcccctt tagtggtgtg
     9661 ggcaatcacc acattcggtt tgccttcctg tttaagctct tcaagcgtcg cgacaaccga
     9721 agccatatca ttgccctgac attcagtaac ctgcataccg aatgccagcc atttctctgc
     9781 cagaggatcg gtgttcatga tttcgcgcgt tggcccggct aattgcagat tatttttgtc
     9841 attaataatg accaggttat caagcccgta atgcgccgcc gccagtgcag cttcccagtt
     9901 actgccctca gccagctcac catcgccagt aattaagaag atacgacgac ggctttgact
     9961 ttttttcgcc gccagcgcaa gtcctacagc cacagggaaa ccatgcccca gtgctccggt
    10021 attcagttca atacctggcg ttttttgccg aaccggatga ccgggtaagt gtgaatttgg
    10081 ctgctgataa gtggaaagcc attccagtgg gaagaatcct gcttccgcca gcacgcagta
    10141 gtaacatcca acagcatggc cttttgactg aatgtatata tcccggtcat cgtcgtgaag
    10201 gcgatccggt gcgacattga gaatacgaaa atagagtgcg gtcagtaatt caacctgaga
    10261 gagatcggcc ccggtgtgtc cacctgccgg gctattggca tttaagcgca cgatatggcg
    10321 gcgtaccgcc cgcgcttttc gctcaagggt ctggatatca tagctatagg gattcattta
    10381 atacctctga atttattttc aacatggaat atttattcca ccagggacta aaaaaccaga
    10441 aatcccaggg cgttgcgcgg caagacttac gctgctgcat catactcagg aacattctgc
    10501 ctccttacac cgtcagttac atgtccgggt aatcatcagt tgacctgata tcatcttgaa
    10561 tatatattca acaatgactc tttattcaat tatgccgcct gttttttatt tttgtccatc
    10621 gctgatttta aggattggtt aaagttagaa ctccatcaca cttaactgtc ttcttttttc
    10681 gcgtatgata aaagcctcta agataagggc ggtacgttaa tgatttacgg ggaaatatat
    10741 ggctaagcag gatgaacagc ggctactggt gaagatcgcc actctttact atcttgaggg
    10801 gcgaaaacag tctgacatcg cccaacttct ctcgctttct cagtcattta tatccagagc
    10861 gctaacccgt tgccagaaag aaggcgtggt taaaattagc gttgtccagc cgtcaaatat
    10921 ctttctcaat ctggaaaaag gcattgaaga gcgttacggc attaaacaag cgattgttgt
    10981 cgataccgaa gatgatgcca gtgatcacac cattaaacgc gccatcggct ctgccgccgc
    11041 gcactatctg gaaacgcgtt taaggccaaa agatttcatt ggcgtctctt cgtggagttc
    11101 tacgattcgc gcgatggtcg atgaagttca cgcccaaaac ttaaaagcca gcggcgttat
    11161 tcagctgctg ggcggcgtgg ggccaaacgg caatgtgcag gcaactattt tgacacagac
    11221 cctggcccag catctgaatt gcgaagcctg gttgcttcct tctcaaagca ttgaaggttc
    11281 ggtagaagag aaaaaacgcc tggtggcaag ccaggatgtg gctgacgtta tcgccagatt
    11341 tgacgacgtc gatatcgcca ttgtcggcat cggtatcctt gaaccctcgc agttgctaaa
    11401 aacctcgggc aactattatc acgaggatat gttacaggta ctggcggatc gcggtgctgt
    11461 gggcgatatc tgcctgcact actacgataa acatggtcag cccgtgttgc aggacgatga
    11521 agatccagtg attggtatgg cactggataa aatcaaaaaa tgcccgaatg tggtggcgct
    11581 ggcgggcggc aaagacaaag ttgccgccat caaaggtgcc atgcagggtg ggtatatcga
    11641 tgttttgatc actgatttcc ccaccgccag aatgctgatt gcggattaat cctcttctcc
    11701 ctttcttctg cccgcctttg cgaaaaaatc ttctcgcaaa ggcgcttgct taaaaagctt
    11761 ttaatatcaa gaaactgaaa ggattcaaca atcctgtttc tcgcggtttt ctcaccccat
    11821 caccacactt ctaagtgtga tagtcagcac atttgatgat tttttctcta gctcaggaag
    11881 aataatctgg ctaaggtata aataaataat cagtatcgaa tatatattca cgccttaaca
    11941 caaaatgtca cagcagggcc tgatgaagta ataacgtgac catgaggaac gcctcatctc
    12001 catccacgcc aggatgcaat gtgactcagg agactaccta tgtttgcagg aaataaagag
    12061 ttcgttattg cgttggatga gggtaccacc aatgcaaaag ccatcgcact tgatggtttc
    12121 ggtaacgtgg tggcgaagtt ttctcaacca ctggctattc agacgccacg tgaaggctgg
    12181 gttgagcaat ccggggaagc gttaatctct gcctcgcttg atgttattgc ccaggccatc
    12241 aaccacgtgg gggcagaaaa tgtagcggct ctggctatca gtaatcagcg tgaaacggct
    12301 atcggctggt atcgtcagag tggaaaacca ctcaatgcgg cgattacctg gcaatgcacg
    12361 cgcagtgccg agttttgtga aacactgcgt cgcgagggca aagagcagca aattaaaacg
    12421 actacgggtc ttcctatcgc cccgctattt tccgcgtcca agatgcgttg gctactggaa
    12481 tctacacctg agggtgtgca acttgccgac cagggtgaaa tttgtctcgg caccattgat
    12541 gcctggttgc tatggaactt gactgctggc gattcatttt gctgcgacta ctctaacgcc
    12601 gcgcgaactc aattacttaa cctgaaaaca ggcgagtggg atgaggaaat gctggcgttg
    12661 tttcacattc cacgcgctgc attgccagaa attaaacctt ccagtggcct gttcggttat
    12721 acccgagggc ttaaaacaat tccggatggt attcccgtca tggcgatggt gggcgattct
    12781 cacgccgctc tttttggtca tgcgttggga gaagcgggat gtgtgaaagc gacctatggg
    12841 accggttctt ccgtcatggc ccccgtcaac tctgctcagt gtgatatcgc cacacttgcc
    12901 acaaccattg cctggcatga tggcgaaaat attatttacg gtctggaagg aaatatcccc
    12961 cacaccggcg atgccgtggc atggatggct gacagcaccg gtttaagtga actgacagac
    13021 gcggagctgg ccctcgaact gaataccctg ccagcctcag tggattcgac tatgggtgtt
    13081 tattttgttc ctgctctcac tggcctgggc gcgccatggg gggatgacag cgctcgcggc
    13141 gtggtgtgtg gcttaagccg tggcgtaaag cgcgcgcatt tgattcgtgc cgcaatggaa
    13201 tcaatcactt atcaaattgc agatgtagtg gaggcaatgc aacagcatga tgattttcac
    13261 ttatcggcgt taatggtgga tggcggcccg accaaaaatg actggttgat gcaatatcag
    13321 gcggatctgc tgggttgccc ggtgatgcgc agtgatgtgc cagagctttc agccattggt
    13381 gcgggcctgc tcgcccgcaa agcgcttatc cccggtaccg ttgcagattt aaaagcgtta
    13441 ttaactgaac acagtgaatt taaaccggac atggcgcgtc atcagcgact gcaaaagaga
    13501 tggcaggagt ggcgtcatgc ggtaaacaga acattatgga aaccggcatc gtcaatttga
    13561 cgaacaagac taaccaacgt aaggagaata ccggcccata aataattcag gctttacgtt
    13621 gtttcaccgt acccgacaat aacaacacag agagaacatt gtcatgaaat tacgtttaac
    13681 actgttaacc gctgcgaccc tgactgctct ttctttttct gctcatgcgg tagaaaaagg
    13741 aaccattatg attatggtca attcactgga taatccttat tacgcctcgg aagcgaaagg
    13801 tgccagtgaa aaagcccagc aactgggata taaaacgacc attctttctc atggtgaaga
    13861 tgtcaaaaaa cagaatgaac tgatcgatac tgcaattggt aaaaaagttc agggcattat
    13921 tttagataat gccgactcca ctgccagtgt tgcagccatt gaaaaagcaa aaaaagcagg
    13981 tattcccgtt atattaatta atcgcgaaat acctgtggat gatgttgcac tggtgcaaat
    14041 tacccataat aacttccagg ctggctcgga agttgccaac gcatttgtcg agaaaatggg
    14101 agagaaagga aaatatgccg aactcacctg taatcttgct gataataatt gcgtaactcg
    14161 ctcgaaatct ttccaccagg ttattgacca gttccctgaa atggtcagcg tggcgaagca
    14221 agatgccaaa ggcacgttaa ttgatggcaa acgaataatg gacagtattc tgcaagccca
    14281 tcccgatgtt aaaggggtga tttgtggtaa tggtccggtt gcactgggag ctatcgcagc
    14341 actgaaagcc gccaatcgca atgacgttat tgttgtcggc attgatggca gtaacgatga
    14401 gcgcgatgcc gtgaaagccg aatcattgca ggctaccgtg atgttacaag cacaagccat
    14461 tgccgcgcag ggtgttaccg atctggataa ttacctgcaa aaaggagtta aaccagccaa
    14521 acagcgggtg atgttccgcg gcattctgat aaccaaagat aacgcagata aagtccagga
    14581 tttcaatatc aaatcctgat cccaggtcgt tgcgccctca caatgagggc gcagaggaga
    14641 atattatgga taaaaaagtc tggctggcgc tggcaattct gagcctgggt ggatgtcgca
    14701 tcgtctcgca gcaggaactt gccgatctca aatccccgcc taatcctcat atggcaaata
    14761 tcgatcagac ctggcagaaa aacatcgtgc cgcaggtggt agaaaatgcg cgtccggttg
    14821 cggagttaat ggcggcgcta caggcagaaa aagatgtcga tgctgcctgt aaaacgctgg
    14881 gctatcgttc tcaggaagaa aacccgtgta tcttctacgt taaggtcgaa ggtggcatca
    14941 ccaatattga tgcgaaatcc cgtagcggaa aaatgaccat cacagatatc agcggcacaa
    15001 atattgtcgt gcagacgggg ccaactcttc gcggcacgct tctgcgagat gcatataaag
    15061 gtgcaagcta tgagcatttt aacgatcagg ttttatttgg tgaatatggt cgtgctatca
    15121 atgagcaagc catcaatatg attcagtcgg caaagcttaa aaccggggaa acggttcagg
    15181 tttatggcgt attttctgca tgggatattc cgcaaactat cccggatata acgcctgcga
    15241 aaatagccag ggcgggaggc gagtaatatg ttaccgcaag aaattgcccc cccccagact
    15301 gatgaatcac ctgtaatcat agaaacgcat gacttatcgc gtatttatcc gggtgtggtg
    15361 gcgttagata acgtgaatta ccgagtctat cgcaataaag taaatgtgct tattggtgaa
    15421 aacggtgcgg gtaaatccac aatgatgaaa atgctggcgg gggttgaaac accttcctca
    15481 ggcaagatca ttcttgatgg taaagaagtt accctaaatt ctacccatca ggccgaaaaa
    15541 catggaatta gtatcatttt tcaggagctg aatttattcc cgaatatgaa tgtcatggat
    15601 aatattttca tggcgaatga attttttcag aaagggaaga ttaacgaaaa atatcaatat
    15661 gaattagcca aatctctatt agaaagactg gaactgaatg ttgatcccta tacccaactg
    15721 ggagagctag gtattggcca tcagcaactg gtggagattg cccgtgccct ttctaaagat
    15781 acccgggtac tgattatgga tgaaccgaca tcggccctca gccagtcgga agtaaaagtc
    15841 cttttcaatg tgatcgcaca actgaagcgc cgtggtgtca ccatcattta tatctcccat
    15901 cgtctggaag agttgatgga gattggcgat ttcatcacca ttttccgtga tggtcgcttt
    15961 attagtgaac gcgccgtgag cgatgccagt attccatgga tcatcgagca aatggtcggt
    16021 gataagaaaa aacatttcga ttatcaacca gctacacaag gtgacactgt actggcggta
    16081 caagggttaa cggcgctgca tcccagcggt ggttacaaac tcaatgatgt gacattcagc
    16141 ctgcataaag gcgaagttat cggcatttac ggcttgctgg gcgcaggacg tactgaacta
    16201 tttaagggat tggtgggcct gatgccctgt cagagtggga agatccagct taatggtgaa
    16261 accatcgaca aatgccattt ccaggcgcgg ctcaaaaagg ggctggcgtt ggtgccagaa
    16321 gatcgccagg gcgaaggcgt cgttcagatg atgtcgattc agtccaatat gacgctatcc
    16381 gatttcagcc tgcaaggttt tcgccgtgcc tggaaatggc tgaatccgca aaaagagagc
    16441 gccagtgtga aagagatgat cctgcaactg gcgatcaaag tcagcgatgc cgaactccct
    16501 attacctcac tcagcggcgg taaccagcaa aaagtggtac ttggcaaagc gttgatgacc
    16561 cagccacagg tggtgtttct tgatgaaccc acacgcggca ttgatgttgg cgcaaaaaca
    16621 gatgtctatc agctggtggg aaaaatggca caacaggggc tggcagtcat gttctcttct
    16681 tcagaactcg atgaagtcat ggcactggca gatcgcattc tggtgatggc tgatggccgt
    16741 attaccgctg atctcccccg gggcgaagtc acccgagaga aactgattgc ggcttcaaca
    16801 cctcaagatt aatcaggctg gaatttctta tgaatcagaa atatatgatc tatatgtacc
    16861 tgttgaaggc cagaacgttt atcgctctgc tattagtgat cgccttcttt agcgtgatgg
    16921 tgcctaactt cctgacagcc tctaacctgt tgatcatgac tcaacacgtt gcgattaccg
    16981 ggctgctggc aatcggaatg acgctggtga tcctgaccgg cggcatcgat ctttccgtcg
    17041 gcgcggtagc ggggatctgc ggaatggttg ccggggctct tctaacaaac ggcctgccat
    17101 tgtggagcgg caatattatt ttcttcaacg tgccggaagt gatcctctgc gtggcggtat
    17161 ttggcatttt agtgggactg gtgaatggcg cggtaatcac ccgctttggt gtcgcgccct
    17221 ttatctgcac cctcggtatg atgtacgtcg ctcgtggctc tgccctgctg tttaatgacg
    17281 gcagcactta tcccaatctc aacggcatgg aaacgctggg caacacgggt ttcgccaccc
    17341 ttggttctgg cacgttttta ggcatttatc taccaatctg gctgatgatt ggctttttga
    17401 ttttaggcta ctggatcacg actaaaacgc cgctcggtcg ttatatctat gccattggtg
    17461 gcaatgaatc tgccgcgcga ctggcaggcg tccccatcgt aaaagttaaa gtgttcgtct
    17521 atgcgttctc cgggttatgc gccgcatttg tcggtttgat tgttgcctcg cagctgcaaa
    17581 ctgctcaccc aatgaccggc aatatgttcg aaatggatgc tattggcgcc acggttcttg
    17641 gtggaactgc actggcgggt ggtcggggac gtgtctctgg ctcgattatc ggtgcctttg
    17701 tcattgtctt cctcgccgat ggcatggtga tgatgggtgt cagtgacttc tggcaaatgg
    17761 tcattaaagg cctcgttatc gtcaccgccg tggtggtcga tcagttccag caaaaattgc
    17821 aaaacaaagt cattttgatg cgtcgccatg aagaaaagat ggccgccgca cctgccgtaa
    17881 aaccgctttc cagttaatta acatcattcc accgggcatt aaattgcccg gcttatacag
    17941 gggaaaaaat atgcaacgta attttcaggg taagactgta gttattaccg gggcatgccg
    18001 gggaattggg gccgggatcg ctgaacgctt cgcccgcgat ggcgcaaatc tggtgatggt
    18061 ttctaacgct gaacgcgtac atgaaactgc agagcaactg cgccaatgtt atcaggctga
    18121 cattttgtca ttagaagtcg atgtgacaga tgaagcgcaa gtacaggcac tctatgaaca
    18181 agcggctgca cgttttggca gcatcgatgt ttccattcag aatgcaggca ttatcaccat
    18241 cgattatttt gatcgaatgc cggctgctga tttcgaaaaa gtgctggcgg tgaacactac
    18301 ggcagtctgg ctctgctgcc gggaagcagc caaatatatg gtgaaacaga atcacggctg
    18361 cctgatcaac acctcttctg gccagggacg tcaggggttt atctataccc cacactatgc
    18421 agccagcaaa atgggtgtga tcggcattac gcaaagcctg gcgcacgagt tagcgcagtg
    18481 gaatatcact gttaacgcct tctgcccggg tattatcgaa agcgaaatgt gggactacaa
    18541 cgaccgcgta tggggagaaa ttctcagtac cgatgacaag cgttatggca aaggcgagct
    18601 tatggcggaa tgggtggaag gtattccgat gaagcgtgcc ggtaaacctg aagatgttgc
    18661 cggactggtt gccttcctcg cctctgacga tgcgcgttat ctcaccggtc aaaccatcaa
    18721 catcgacggc ggcctgatta tgtcgtaatg cccctgcggg ctactaagag agatatcaaa
    18781 atgatcgcaa aagattttgc tcaacaattg tttgatctcc acgatcgcgt cgcctttgtc
    18841 actggcgcag gtagcggtat cggacaaatg atcgctctgg ctttagccag cgcaggctca
    18901 cgagtggtgt gttttgatct acgggaagat ggcggactta aagagacggt taataatatc
    18961 gaagcgcttg gcggacaagc cttgtattac acgggcgatg ttcgggaatt gagagatctt
    19021 cgcgcagcag tcgctctggc aaaaacacat tttggtcgcc tggatattgc cgtaaacgcc
    19081 gctggaatcg ctaacgcaaa cccggcgctg gagatggaaa gcgagcaatg gcaacgcgtt
    19141 atcgatatca acctgactgg tgtgtggaat tcctgtaagg cggaagctga gttaatggta
    19201 gagaacggag gcggttcgat tataaatatt gcgtcaatgt ccgggattat tgtcaatcgt
    19261 ggtctggaac aggcccacta caacagttcc aaagccggcg tgattcattt atctaaaagt
    19321 cttgcgatgg aatgggtgag taaggggatc cgcgtgaact caatcagccc aggatatacc
    19381 gcgacaccaa tgaatacacg acctgaaatg gtacatcaga ctcgggagtt tgaaagccag
    19441 acgccgattc aacggatggc gaaagtggaa gagatggcgg gtccagcgct gttccttgcc
    19501 agcgatgcgg cgtcgttctg cacgggtgtg gatctggtgg tcgatggtgg ctttgtctgc
    19561 tggtaaaaat ggtttcgccc gcagccatca ctgaccgcgg gcgaaagtgt aaagcaggtg
    19621 ccttaccacc ctgacctgac aaccggatat gcggggatta ctccccgcac agcggctact
    19681 tcttcggttc gtaagcgaga acagccttaa actcaatatt acgttccttc accgtaaact
    19741 cacacagcga gtctccaacc agaagcgtaa atcccagcac cgtcaaacac agcactatga
    19801 ctgccgcaag ggcatatttc gtcagcatgt ttatattgcc tccatgcgaa gaagggacta
    19861 ccatccaaat tgttgggttt ggagtggcag ccccaggatt gatagaaata tctcctgggg
    19921 cttcatcttt ccagacctca gagtaaacct gaaaccggaa agcttcaggc acccgccgtt
    19981 atcttaccct tccttagcaa aatagctgtt tatttataca gtgagtcctg tttcggactt
    20041 ctgttggcaa aactgatcat tgcccttaaa acgacatcgc ccgcagccat cactgactgc
    20101 gggcgaaagt gtaaagcagg tgccttacca tcctgacctg acaaccggat atgcggggat
    20161 tactccccgc acaacggcta cttcttcggt tcgtaagcga gaacagcctt aaactcaata
    20221 ttacgttcct ttacggtaaa ctcacacagc gagtctccga ccagaagcgt aaatcccagc
    20281 accgttaaac acagcactat gactgccaca agggcatatt tcgtcagcat gtttatattg
    20341 cctccatgcg aagaagggac taccatccaa attgttcagg tttgggtagg tagccccagg
    20401 gttgatagaa atatcgtcct ggggcttcat ctttccagac ctcagagtaa acctgaaacc
    20461 ggaaagcttc aggcacccgc agttatctta cccttcctca acaaaatagc tgtttattta
    20521 tacagtaaat cctgtttcgg acttctcact ccccatttga tcaactgaag ctgaacccgt
    20581 ttttatctgc atgcctcaat cgtcaaaatt cttcgccacc agccagctat gacgcaactg
    20641 gtcacattgc aaaatgtagc caatttgctt cagtcaccat caccaaaaaa agaaacattc
    20701 cagaacttcc gcatctcacc cagccgctcc cacttctata ctgaatagaa aacgccaata
    20761 taaaagaaac ctatgccatt acccgatttt catgtttctg aaccttttac cctcggtatt
    20821 gaactggaaa tgcaggtggt taatccgccg ggctatgact taagccagga ctcatcaatg
    20881 ctgattgacg cggttaaaaa tcagatcacg gccggagagg taaagcacga tatcaccgaa
    20941 agtatgctgg agctggcgac ggatgtttgc cgtgatatca accaggctgc cgggcaattt
    21001 tcagcgatgc agaaagtcgt attgcaggca gcggcagacc atcatctgga aatttgcggc
    21061 ggtggcacgc atccgtttca gaaatggcag cgtcaggagg tgtgcgataa cgaacgctat
    21121 caacgaacgc tgcaaaactt tggctatctc atccagcagg cgaccgtttt tggtcagcat
    21181 gtccatgttg gctgcgccag tggcgatgac gccatttatt tgctgcacgg cttgtcacgg
    21241 tttgtgccgc actttatcgc cctttccgcc gcgtcgccat atatgcaagg aacggatacg
    21301 cgttttgcct cctcacgacc aaatattttt tccgcctttc ctgataatgg cccgatgccg
    21361 tgggtcagta actggcaaca atttgaagcc ctgtttcgct gcctgagtta caccacgatg
    21421 atcgacagca ttaaagatct gcactgggat attcgcccca gcccccattt tggtacggtg
    21481 gaagttcggg tgatggatac cccgttaacc cttagccacg cggtaaatat ggcgggatta
    21541 attcaggcta ccgcccactg gttactgacg gaacgcccgt ttaaacatca ggagaaagat
    21601 tacctgctgt ataaattcaa ccgtttccag gcctgtcgct atgggctgga aggcgtcatt
    21661 accgatccgc atactggcga tcgtcggtca ctaacggaag ccaccttgcg tttactggaa
    21721 aaaatcgccc cttctgcaca taaaattggc gcatcgagcg caattgaagc actgcaccgt
    21781 caggtcgtca gtggtctgaa tgaagcgcag ctaatgcgtg atttcgtcgc cgatggtggc
    21841 tcgctaattg ggctggtgaa aaagcattgt gagatctggg ctggagaata agtcgggaat
    21901 tgccttacag aagaatatcc ctgacaatgg tgttttaact tacatttaac aatgttatga
    21961 aacactcttt agaaaccttg accaccgcag caggcatttt gctgatggct ttcctctctt
    22021 gcctgctgct gcccgccccc gcactggggc tgacgctggc acaaaaactg gtgaccacgt
    22081 tccatctgat ggatcttagc cagctttaca ccttattgtt ttgtctgtgg tttttagtgc
    22141 tgggcgctat tgagtatttt tttctacgct ttatctgccg acgctggttc tcgctggcgg
    22201 attaaacgtc gattatcgcc gaacttcgcg gcatactttg cttattctcc ttcgcctaaa
    22261 ggaacgttta tggataagca atcactgcac gaaacggcga aacgcctggc tcttgagtta
    22321 ccctttgtcg agctttgctg gccttttggc ccggagttcg atgtttttaa aattggcggc
    22381 aagattttta tgctgtcgtc ggagctgcgc ggcgtcccct ttatcaatct gaagtccgat
    22441 ccacaaaaat ccctgctaaa tcagcaaata tacccaagca tcaagccagg gtatcacatg
    22501 aataaaaagc actggatttc ggtgtatccc ggcgaggaaa tctccgaagc gttacttcgc
    22561 gatctgatca acgattcgtg gaatctggtg gttgatggtc tggctaaacg cgatcaaaaa
    22621 cgactgcgtc ctgactcaac cgtaaatcaa tagcgcattt ttctcgctta ccatttcatc
    22681 tcgatccttg taatctgctg gcacgcaaaa ttactttcac atggagtctt tatggatatc
    22741 atttctgtcg ccttaaagcg tcattccact aaggcatttg atgccagcaa aaaacttacc
    22801 ccggaacagg ccgagcagat caaaactcta ctgcaataca gcccatccag caccaactcc
    22861 cagccgtggc attttattgt tgccagcacg gaagaaggta aagcgcgtgt tgccaaatcc
    22921 gctgccgata attacgtgtt caacgagcgt aaaatgcttg atgcctcgca cgtcgtggtg
    22981 ttctgtgcaa aaaccgcgat ggacgatgcc tggctgaagc tggttgttga ccaggaagat
    23041 gccgatggtc gctttgccac gccggaagcg aaagccgcga acgataaagg tcgcaagttc
    23101 ttcgccgata tgcaccgtaa agatctgcat gatgatgcag agtggatggc aaaacaggtt
    23161 tatctcaacg tcggtaactt cctgctgggc gtggcggcgc tgggtctgga cgcggtgccc
    23221 atcgaaggtt ttgacgccgc catcctcgat gaagaatttg gtctgaaaga gaaaggctac
    23281 accagtctgg tggttgttcc ggtgggtcat cacagcgttg aagattttaa cgctacgctg
    23341 ccgaaatctc gtctgccgca aaacatcacc ttaaccgaag tgtaattctc tcttgccggg
    23401 catctgcccg gctatttccc ctcagattct cctgatttgc ataaccctgt ttcagccgtc
    23461 atcataggct gctgttgtat aaaggagacg ttatgcagga tttaatatcc caggttgaag
    23521 atttagcggg tattgagatt gatcacacca cctcgatggt gatgattttc ggtattattt
    23581 ttctgaccgc cgtcgtggtg catattattt tgcattgggt ggtactgcgg accttcgaaa
    23641 aacgtgccat tgccagttca cggctttggt tgcaaatcat tacccagaat aaactcttcc
    23701 accgtttagc ttttaccctg caggggatta tcgtcaatat tcaggcggta ttctggctgc
    23761 aaaaaggcac cgaagcggca gatattctga ctacctgcgc gcagttgtgg atcatgatgt
    23821 atgcgctgct ttcagtcttc tcgttgctgg atgttatttt gaatctggcg cagaaattcc
    23881 cggcggcatc tcagttaccg ctgaaaggga tatttcaggg gattaaactg atcggcgcga
    23941 ttctggtcgg cattttgatg atctcgctgc tgattgatca gtcgccagcg attctgatca
    24001 gcggtcttgg tgcaatggct gccgtgctaa tgttggtatt taaagatccg attcttggtc
    24061 tggtggcagg tattcagctt tccgcgaacg atatgctgaa actgggcgac tggctggaga
    24121 tgccgaaata cggcgcggat ggcgcggtga tcgatattgg gttaaccacc gtcaaagtgc
    24181 gtaactggga caataccatt accactattc ccacctggtc tctggtttct gactccttta
    24241 aaaactggag cgggatgtca gcttctggcg ggcgacgtat taaacgcagt atcagtattg
    24301 atgtcaccag tattcgtttt cttgatgaag acgaaatgca acgtctgaat aaagcgcatt
    24361 tgttaaagcc ttatttaacc agccgccatc aggaaattaa tgagtggaat cgccagcaag
    24421 gttctacgga gtcggtatta aacctgcgcc gaatgaccaa tattggaacc tttcgtgcct
    24481 atctgaacga atatctgcgg aatcatccgc ggattcgcaa agatatgacc ttaatggtac
    24541 gccaactggc tcctggtgat aacggtttac cgcttgagat ctatgcgttt accaacaccg
    24601 tggtgtggct ggaatatgaa agcattcagg ctgatatatt cgatcacata tttgcgattg
    24661 tcgaagagtt tggtctgcga cttcatcagt cgccaaccgg caatgatatt cgctctctgg
    24721 cgggtgcatt taagcagtaa ttaaaaaaac cgctctcatc gaatgggtga gagcggcttt
    24781 cagattgttg acatcatttt gtcggatgcg gcgtgaacgc cttatccgac ctacagaatc
    24841 gttacaaatt gtaggcccga taagcttgcg catcgggcaa atcagcctca catcagctta
    24901 attcactttc ctacgcaatg ttttaaacgc cacaaacagg aacacaatcc acaccggcag
    24961 cagaatcgct gacaagcgca tatcatccat cgtgcacatc agcagcaaaa tcatgccgag
    25021 gaaggcgatg caaagatagt tgccgaacgg atagagcagc gccttaaatt gtgtttcacg
    25081 cccctgacgt cgcatcgccg cgcgaaaacg cagatgcgcc aggcagatca taatccagtt
    25141 caacagcagc gttgctacca ccagcgccat cagcagacca aacgcttttt gcggcagcag
    25201 atagttgatt aacaccacca gcgaagtgat cgctccggaa agcatcagcg agttaatcgg
    25261 cacgccgcga cggctgacgc gagtcaaaaa ctttggcgca ttaccctgca cagaaaggcc
    25321 aaacagcatg cggctgttag agtaaacccc gctgttatac accgacagcg atgccaccag
    25381 aatgacgaag ttcagcgcag aagctaccac gttgctgtcg agattatgga aaatcatcac
    25441 aaacgggcta ctattggatt tcacttccac ccacgggtag agcgccagta aaaccaccag
    25501 tgaaccgata taaaacagca ggatgcgata caccacctga ttcactgctt tagggatgct
    25561 tttttccgga tcgcgcgctt cagccgcagt aatcccaatc agctccagac cgccgaagga
    25621 gaacataatt accgccagcg acaaaatcag tccattccag ccggtggcaa agaaaccgcc
    25681 gtagcgccag aggttatcga tactggcttt ctcaccgccg tgcccagaaa acagcagcca
    25741 caggccaaag ccgatcatac cgatgatcgc cagcacttta atcagcgcaa accagaactc
    25801 ggtttcgcca tataagcgca cgttcaccag gttaacggcg ttgatgataa taaagaaggc
    25861 ggcagcccaa atccacgttg gaacatccgg gaaccagtac tgcatataga tgcccgcagc
    25921 ggtcagctct gccattccca ccagcacgaa cattacccag tagttccagc cagagaggaa
    25981 gcccgcaaac ggtccccagt atttataggc aaagtgggca aatgaaccgg ataccggctc
    26041 ttcgaccacc atctcgccga gctggcgcat aatcaggaaa gcgatgatcc cggcgacgcc
    26101 gtagcccagc aatacagccg gacccgccat ctgaatcgcc gggccaatgc caagaaacag
    26161 accagtacca attgcgccac ccagcgcaat cagttgaata tgacggttat gtaatccgcg
    26221 atgaagcgtc ggctcttgat tcgacgcagt atcttccgat acggttgacg cgtttttcac
    26281 gcctttcccc tgtgtgtctt ttttgttgag gggcaccttt taacatttag tgcccatcgt
    26341 cgcaagacac aatccacacg gttaaacggg gtatcctgct tttatttccg tacccgatgt
    26401 cggtgcagcc acatcagctt atacgccgcc gggataataa acagcgacag caaaggcgcg
    26461 gtgatcatgc cgccaatcat cggcgcggca atccggctca tcacctctga ccctgctcct
    26521 gttccccaca gaatcggcag cagaccagcg ataatcaccg ccaccgtcat cgcttttggg
    26581 cgcacgcgca gtaccgcgcc gtgatataac gcctcatcca gcttctgctc gctgaatgtt
    26641 tgcggattat tcaacgacgg ctcggcctct atggcgtgac gtaaatacat cagcatcacc
    26701 acgccaaatt cggcggcgac cccggcaagg gcaataaaac cggtgcccgt cgccacggaa
    26761 agatgaaagc ccatccacca cagcaaccag atgccgccca ccagcgcaaa cggtacgctg
    26821 ctgataatca gcaacgcttc gcccacccga cggaacgcca gatacaacag cacgaagatg
    26881 atcatcagcg tcatcggcac catcagtttc agcttatggt tggcgcgctc cagtagctcg
    26941 aactgcccgg agaatgccac gctggtaccc ggttttaact gcactttttc agctatcgct
    27001 ttttgcagat cgtgaaccac cgacaccatg tcacgatcgc gggcatcgat ataaatccag
    27061 ctcgtcgggc gcgcattctc ggttttcagc atcgacggtc cggtagagac tttaacgtcg
    27121 gccacgtccg ccagagtgat ttgctgcttc atcggcgtga ggatcggcaa ctggcgcagt
    27181 gcctgcgggc tatcgcgcca gctttgcgga taacgcagat taattggata acgagcaatc
    27241 ccttccaccg tttcgccaac catcgccccg cccaccgcag aagtcacaaa caactgcaca
    27301 tccgccaccg tcataccgta acgcgcggct ttttcacggt taatctcaac gttgatataa
    27361 cgcccacctt ccagacgctc agcaagcgca gaagccacgc ctggcaccgt tcgcgccact
    27421 tcttcaattt gctccgccat cgtgtcgata tccgccagca cagtgccgga aactttaatg
    27481 ccgatagggc ttttaatgcc agtcgagagc atatcgatac ggttacgaat tggcggcacc
    27541 cacagattcg ccagccccgg cagacgcacg gtgttatcca gttcctcaat gattttgtcc
    27601 atcgtcatgc caggccgcca ctgatcctgc ggcttaagct ggatagtcgt ttctaccatc
    27661 tccagtggag cagaatcggt ggcggtttcc gctttcccgg ttttgccaaa tacccgcgcc
    27721 acttccggta cgctcataat cagcttgtcg gttttttgca gcatgctcgc cgcctctgct
    27781 gcggaaatcc ccggcagcgt cgatggcata tacaacaagt cgccttcatt gatctgcggt
    27841 aaaaattccc cgccaacttt attgagcggc cagagaaccg tcagcaccga aagcgctgcc
    27901 accaacagcg tggttttcgg ccagtgcagt actttcagca acagcggatg ataaacacga
    27961 atcaaaaagc gattgagcgg gttactgctt tccggcggaa ttttgccacg gatccagtag
    28021 cccatcagga tcgggatcac tacaatcgcc agtagcgccg cgcccgccat cgcataggtt
    28081 ttggtgaacg ccagcgggcc aaacagacga ccttcctgcc cttccagggt gaagatcggg
    28141 ataaacgaca gcgtgataat cagcagactg ataaacagcg ccggccccac ttcaacagat
    28201 gcatcggtga tcacctgcca gcgcgtttta ttatccagcg tggcgtcagg atgctggtgc
    28261 agccactctt ccagccgttt atgcgcattc tcgatcatga cgatagcggc atcgaccatc
    28321 gccccgacgg caatcgcaat gccacccaac gacataatat tggcgttcag tccctgaaag
    28381 tgcatgacaa taaaagcaat acacaacccc agcggcaacg aaataatcgc caccagcgcc
    28441 gagcgcacat gccagagaaa cagcgcacag actaccgcca ccacaataaa ctcttccagc
    28501 aacttgccgc tgaggttgtc aatggcgcgg tcaatgagct ggctgcgatc gtatgttgta
    28561 actatctcca caccttccgg cagactgctt ttcagcgttt ccagtttgtc cttcacggcg
    28621 gcgatcactt cacgggcgtt tttgccggaa cgcaggatca ccaccccgcc cgccacttcg
    28681 ccttcgccgt tcagttcggc aatgccccgg cgcatctccg ggccaacctg gaccttcgca
    28741 acatcacgca gataaacggg cacgccattt tcactggctt ttaaaacgat gtgattaaag
    28801 tcgtcgagcg tttgcagata gccgctggcg cgcaccatat attccgcttc cgccagttcg
    28861 atcgacgaac cgcccgcttc ctggtttgaa gcatccagcg cgctttttac gtcggcgaga
    28921 ctgatgccat actgcgccag gcgctgagga tcgataacca cctgatactc tttcaccaca
    28981 ccgcccaccg acgccacttc cgcaacgtca gggatggttt ttagctcata tttgagaaac
    29041 cagtcctgca atgagcgtaa atcggccaga tcgtgcttac cgctgcgatc caccagtgca
    29101 tattcataga tccagccaac acccgtggca tctggcccca gctcggcgct gactcccgca
    29161 ggcagcttac cctgtacctg gttgaggtac tccagcaccc gcgagcgcgc ccagtacgga
    29221 tcggtgccat cttcgaaaat gacatacaca taagagtcgc caaactgcga gaaaccgcgc
    29281 acagtcttcg cgccaggcac cgacaacatg gtggtggtta gcggataagt cacctgattt
    29341 tcaacgattt gcggtgcctg accgggatag ctggttttaa taatcacctg tacatcggaa
    29401 agatccggca gtgcatccac tggcgtgtta atgatggtcc aggtgcccca gatactcaga
    29461 aacaacgcgc ccatcagcac cagaaaacgg ttcgccaccg agcgacgaat aatccattca
    29521 atcattggct attccctcaa tgcgcatggg tagcactttc agagcgcatc cgctccagtg
    29581 cgccagaaat attggcttca gaatcaatca ggaacaggcc gctggaaacc accttttcac
    29641 cttccgccag accagagcgt aacgcggtga cgccttgcga tgcctggaaa acggcaacgc
    29701 gtttcggtac aaagcgcccg tcagcatcaa cggtaatcac ccgctgttcg ttgccggtat
    29761 caatcagcgc ctgtgacgga atgagcagca tcggttcgct ggcggtattg agttgcaacc
    29821 cggcgttcat gcccggtttt agcgcctcgt cggcgttgtc gacttccaga cgcagctgca
    29881 gcgtgcgggt cgcggcatcc acgccgggta gcagcgtcca tttgcggatg gtgagtgttt
    29941 tatcaggtcg cgccggaacg gtgagcgtaa actgcgaggc atctttcacc agccatgcga
    30001 tggactccgg gatcgcagca gtgacccaca ccgggtccat accctgaatt ttcgcgacca
    30061 cgttatcttt ggcgatattc attcccgcgc gcagatcaaa cgcggtgatc acgccatcaa
    30121 tgggcgcttt gagcgtaaag cgagtctgga ttttttgcgt ggcgatcaga cggcgaatat
    30181 cagcctccgg cattcccgcc agtcgcagtc gctcaagaat gccttcagtc tgggtcgccg
    30241 taccgccggt ttcgcgcagc agtaaatact cactctgcgc ttccacccag tcgggaatgg
    30301 tcaggtcgag aagcggcgcg cccttttgca ctttatcgcc cacggtaagc ggatacacct
    30361 tgtcgataaa cccggcggcg cgggcctgca caatggcata ctgatactcg ttgtaactga
    30421 catttgccgg gaaactctgg gcaaaagtca gcggcccacg cgtgacggtc gccgttttca
    30481 cccccagatt ctgcgtctga gtcgggtcaa tgcgcacacc agatgcagaa ctctcttcat
    30541 cggcatattt cggcaccaga tccatatcca taaacggcga tttccctggt ttatcgaacc
    30601 gcgtattggg atacatcggg tcgtaccaga ataagacttt acgttctgcg gtcgacgttt
    30661 tttctgcggg cggttcctcc tttgcaaacc aggtaaaacc tgccgcagaa ataataccgc
    30721 ccgcgatcat gctgccgata ataagcgcga tttttttcat ctcattaaac ccggattact
    30781 ggctgacttt aatatcctgt aataaagaaa gattgccctg ctggacaaaa ttaaacgcta
    30841 ctttatcgcc ggttttaatt tcgctcattt tcgtctgcgg ggtgatggta aagcgcatag
    30901 tcatctccgg ccagttcacg gcagcaatcg gatcgtgatg gatggtgatt tttttgcttt
    30961 ccagatcaac accttttacc acgccagtgg cgctaataac ctgtggttgt gcttcgctca
    31021 tggtttcatg atgatgttcg ttagcctggg cattaaagcc aataacggta aacagactga
    31081 acattgcgac ttgcagtgct tttttcattt attctctcct ggagttaaaa attattgctg
    31141 ccaaccgccc cctaaggcgg tatataaaga aatttcgtta acctgacggg cataattcag
    31201 gtcaagtaaa gtttgtcggg ttgcaaataa agaacgttca gcatccagca cttccagata
    31261 actgactgcg ccgtgctgat ataatgcccg cgcccgttgc agggtaattt gcagcgacgc
    31321 aagataacgc tgctgggcgc tgatttgatc gttcaggctt tgacgtaatg ccagcgcatc
    31381 tgccacttct ttaaaggcgt tctggatttt ctgttcataa ttcaccaccg actgctgctg
    31441 gcgaatttcg gcgatatcca gattggcctg gttgcgtccg gcattaaaaa tggggatctc
    31501 aattttagga ataaaattcc acatcccgct gctggcgtta aataatgacg acagatcgct
    31561 gctggcggtt gatattccgc tggtcaggct gatagacggg aaaaatgccg cacgcgcagc
    31621 accaatattg gcattagccg ccattaacgc gtgttcagct tccataatat cagggcgctg
    31681 caataagatt tgcgacgaca agcccgccgg taatttaacg ctttgcaggc tgtcgctgtt
    31741 tactgtctgc gcttgcggca gcttgccgta gcttcccaat aacagttgca atgcattatt
    31801 cgcctgcgcc agttccccct gacgtttagc gatgtcgctg cgggtacttt ctatcacccc
    31861 gcgagcctgt tccagcgcca gaacattgct gctaccggtc aacagttgtt tttcgacaaa
    31921 cgcatatgac tgctgataat tacgcagcgt ttcttcggct atttgcaatt gcgcatacgc
    31981 cagttgctga ttgaaatagc tttgcgcgac attagaaacc agcaaaatat gcaccgcgcg
    32041 ctgagcttcc tcagtggcta aataattttg gcgctcggct tcgctcatgt tctttaagcg
    32101 accgaaaaaa tcgagatcaa agctggcgtt aaggccagta gagaactccc gcgtcgtggc
    32161 tgaatcgcct ttaagattgc cgctccagct gccgctgccc tcgccattga gctgtgggta
    32221 gcggtcggca tcggtcagac gatattgcgc ccgcgcttcc tgcactttca gcgccgccat
    32281 gcgcaaatcc cggttattca ccaacgcctc gctaatcagc gtcttcacct gattatcaac
    32341 aaaaaaggtg cgccagcccg cgttctggta gttatctgct gcgttaacca ggccgttctg
    32401 gctgagtgag aactgctgcg gcacgggcat tgccggacgc tgataatccg gtgccagtga
    32461 acaaccggtt agcgcaaggg ccacacaaaa tggcagaagt ttacaaggag acataggctc
    32521 ataatttctg gtgattttat gctgccaact ttactcgcca ggctctgatt ttccggtgac
    32581 aggaaaatga caaaattgtc attttgccaa taagcgattg ccatctgatc ccgctactct
    32641 agaattgccc gggcaacatg cggaggaaat atgaaactgt tgattgtcga agatgaaaag
    32701 aaaaccggag aatacttgac caaagggtta accgaagccg gttttgtggt cgatttggcc
    32761 gacaacgggc tgaatggcta ccatctggcg atgaccggtg attatgatct gataatcctc
    32821 gatattatgc tgccggacgt gaacggctgg gatatcgtgc gcatgttgcg ttccgccaat
    32881 aaggggatgc cgattctgtt gcttaccgcg cttggcacca ttgaacatcg cgtcaagggg
    32941 ctggagttgg gggcggatga ctatctggtg aagccgttcg cttttgctga actgctggcg
    33001 cgggtgcgca ctctactgcg gcgcggggcg gcggtgatta tcgaaagtca gtttcaggtt
    33061 gccgatctga tggtcgatct cgtcagccgc aaagtcaccc gcagcggcac gcgcatcact
    33121 ctgaccagta aagaatttac tctgctggag tttttcctcc gccatcaggg cgaagtgctg
    33181 ccccgctcgc ttatcgcctc gcaggtatgg gacatgaatt ttgacagcga taccaacgcc
    33241 attgatgtgg cggtgaagcg gctgcgcggc aaaatcgaca acgactttga gccaaagttg
    33301 attcagaccg tgcgcggcgt gggttacatg ctagaggtgc cggatggtca gtaagccatt
    33361 tcagcgcccg ttttcgctgg caacccgcct gacctttttt atcagcctcg ccaccatcgc
    33421 ggcgtttttc gcctttgcat ggatcatgat ccactcggta aaagtgcatt ttgccgagca
    33481 ggatattaat gatttaaagg agattagcgc cacccttgaa cgggtactta atcaccctga
    33541 cgaaacgcaa gcccgacgct taatgacgct ggaagatatc gtcagtggtt attccaatgt
    33601 gttgatctcc ctggcaaata gccacggtaa aacggtgtat cactccccag gtgcgccgga
    33661 tatccgcgag tttacgcgtg acgccatacc cgataaagac gcgcaggctg gcgaggtgta
    33721 tctcctttcc ggcccgacga tgatgatgcc agggcacggt cacgggcata tggaacacag
    33781 caactggcgg atgattaact tgccggttgg cccgctggtt gacggcaaac cgatttatac
    33841 gctctacatc gcgctttcga tcgattttca tcttcattac atcaatgatt tgatgaataa
    33901 acttattatg accgcatcgg taatcagcat cctgatcgtc tttatcgtgc tgttggcggt
    33961 acataaaggt cacgcgccga tccgcagcgt cagccgtcaa atccagaata tcacctcgaa
    34021 agatctcgac gttcgccttg acccgcagac cgtgcccatt gagctggaac agctggtact
    34081 gtcgttcaac catatgatcg agcgtattga ggatgtcttt acccgccagt ccaatttctc
    34141 agcggatatc gcccacgaaa ttcgcacacc tattactaat ctcattacgc aaacggaaat
    34201 cgccctcagc cagtcgcgca gccagaagga gctggaagat gtgctctact ctaatctcga
    34261 agagctgacg cgaatggcga aaatggtcag cgatatgctg tttctcgccc aggccgataa
    34321 caaccagcta atccccgaaa agaaaatact caacctggcg gatgaagtcg gcaaagtgtt
    34381 cgattttttc gaggcgttag cagaagatcg cggcgtggaa ctgcggtttg ttggcgacga
    34441 gtgtcaggtt gcgggcgatc cgctgatgct gcgtcgggcg ttaagcaacc tgctttctaa
    34501 tgccctgcgt tatacgccaa ccggagagac aattgtcgtg ggctgccaga tggtcgatca
    34561 cctggtgcaa gttaccgtcg aaaaccccgg tacgcccatt gcgcccgaac acttaccgcg
    34621 attgtttgac cgtttctacc gcgttgatcc ttcccgccag cgaaaaggcg aaggtagcgg
    34681 cattggcctg gcgatagtga aatcgattgt tgtcgcgcat aaaggcactg ttgcagtaac
    34741 gtcagatgca cgggggacaa ggtttgttat catattaccc gcttaatcct tcagcaaacg
    34801 gcaactttta taaccagtgt aaaaataaca tgccgcaata gtaataccta ttaatgataa
    34861 aaagctgtca caattcataa aaaaccttaa tatacgccac cctaaacata accagcgtta
    34921 atgtaaggtt tttgtgtgga ctggcttctt gatgtttttg ctacctggct ctacggctta
    34981 aaagtaatcg cgataacgtt agcggtcatt atgttcatca gcgggctgga cgattttttt
    35041 attgatgtcg tctactgggt acgccgcatt aaacgcaagt tgagtgttta tcgccgctac
    35101 ccgcgaatga gttaccgcga actgtataaa ccagatgaaa aaccgttagc gattatggtt
    35161 ccggcgtgga atgaaacggg cgtcatcggc aatatggccg agctggcggc gaccacgctc
    35221 gactacgaaa actatcatat ctttgttggc acctacccca acgaccccga tactcagcgt
    35281 gatgttgacg aagtgtgcgc tcgcttcccg aacgtgcata aggtagtctg cgcgcgtcct
    35341 ggccccacca gcaaagccga ctgtctgaac aacgtgctgg acgccatcac ccaatttgaa
    35401 cgtagcgcca atttcgcttt tgctggtttt attctgcatg acgccgaaga tgtgatttca
    35461 ccaatggaat tgcgtctgtt caactatctg gtcgagcgta aagatctgat tcagatccca
    35521 gtgtatccgt tcgaacgcga atggacgcac ttcaccagca tgacctacat tgatgagttt
    35581 tcagaactgc atggcaaaga tgttccggtg cgtgaagccc tcgccggaca ggtgcccagc
    35641 gcaggagtcg gcacctgttt cagccgccgc gccgtgaccg cactgttagc tgacggtgac
    35701 ggtattgctt tcgacgtgca gagtctgact gaagattacg acattggctt ccgcctgaaa
    35761 gagaaaggta tgacggaaat ttttgtccgt tttccggtgg tggacgaagc caaagaacgc
    35821 gagcagcgta aatttttaca gcatgcgcgg acgtcaaaca tgatctgcgt gcgcgaatat
    35881 ttccccgata ccttttcgac tgcggttcgg caaaaatctc gctggatcat cggcattgtt
    35941 ttccagggct ttaaaaccca caaatggacc tccagcctga cgctgaacta ctttctctgg
    36001 cgcgaccgca aaggggcaat cagtaacttt gtcagcttcc tcgcgatgct ggtgatgatc
    36061 cagcttttgc tgttgctggc gtatgaaagt ttgtggcccg atgcctggca tttcctttct
    36121 attttcagcg gcagcgcatg gttaatgact ctgctgtggc taaactttgg cttgatgatt
    36181 aaccgcatcg tgcagcgggt gattttcgtt actggctact acggcctgac gcaggggcta
    36241 ctatctgtcc tgcgtctttt ctggggcaac ctgattaact tcatggccaa ctggcgtgcg
    36301 ttaaaacagg tacttcaaca cggcgatcca cgtcgtgtcg cgtgggataa aacaacgcat
    36361 gacttcccca gcgtgactgg cgatacccgc tcgttgcgtc cgttaggtca aatcctgctg
    36421 gaaaatcagg tcatcactga agaacaactc gatacagcac tgcgtaatcg cgtcgaaggt
    36481 ctacgcctgg gcggttcaat gctgatgcag gggctgatta gcgccgagca actggcacag
    36541 gcgctggcag agcaaaacgg cgtggcgtgg gaatccatcg atgcctggca gatcccttcc
    36601 tcgctgattg ccgaaatgcc ggcctccgtg gcgctgcatt atgcggtact gccgctgcgt
    36661 ctggacaatg atgagttaat tgtcggcagt gaagatggta ttgacccggt ttcgctggcg
    36721 gccctgacgc gtaaagtcgg acgcaaagtg cgttacgtca ttgttctgcg gggacaaatt
    36781 gtcaccggat tgcgccactg gtatgcacgc cgacgcggtc acgatccgcg ggcaatgttg
    36841 tacaatgcag ttcagcatca gtggctcacg gaacagcagg ccggtgaaat ctggcggcaa
    36901 tatgtgccgc atcagttcct gttcgcagaa atactgacca cgctcggtca tattaatcgt
    36961 tcagcaatta acgtgttgtt attgcgccat gaacgcagtt ctctgccgct cggcaagttt
    37021 ttggtcaccg aaggcgttat cagccaggaa acgttggatc gcgtcctgac aattcaacgc
    37081 gaattacaag tttcgatgca atcactatta ctcaaagcag gtttaaacac agaacaggtt
    37141 gcacaactgg agtccgaaaa tgaaggagaa taaccttaat cgcgtcatcg gatggtctgg
    37201 tttactgctg acgtctttat tgagtaccag cgcactcgca gacaatatcg gcaccagcgc
    37261 agaagagctg gggctgagcg attatcgcca ttttgttatt tatccccgtc tcgataaggc
    37321 gctgaaggca cagaaaaata acgacgaagc aaccgccatc cgcgaatttg aatatataca
    37381 ccagcaggtg ccggataata ttccgctgac tttatacctt gcggaagcct atcgccattt
    37441 tggtcatgat gaccgggcgc ggctgttgct tgaggatcaa ctgaaacgtc accctggaga
    37501 tgaccggctt gagcacagtc tggcggctat tcctgttgaa gtgaaaagcg ttacgactgt
    37561 tgaagaactg cttgcccagc aaaaagcgtg cgatgctgcg ccgaccctgc gttgtcgcag
    37621 tgaagtcggg cagaatgccc tgcggctggc acaattacct gtcgccagag cgcaactgaa
    37681 cgatgcgacg tttgctgcat cgccggaagg aaaaacgttg cgaaccgatc tgctgcaacg
    37741 cgcaatctac ctgaaacaat ggtcccaggc agatacgcta tataatgaag cacgccagca
    37801 gaacacatta agcgcggcag aacgccgtca gtggtttgac gtgcttcttg ccgcgcagct
    37861 ggatgatcgg atcctggcgc tgcaatcaca ggggatcttc accgatcctc agtcatatat
    37921 tacttacgcg accgcgctgg cttatcgtgg cgaaaaagca cgcctccagc attatctcat
    37981 tgaaaataag ccgctgttta ccacggacgc acaagagaaa agttggctct atctgttatc
    38041 taaatacagc gccaaccccg ttcaggcgtt ggcgaattat acggtgcagt ttgccgataa
    38101 ccgccagtat gttgttggcg cgacgctacc ggtgctgtta aaagaaggtc agtacgacgc
    38161 agcgcaaaaa ctactcgcca ccctccccgc caatgaaatg cttgaggagc gttatgccgt
    38221 tagcgtagcg acccgtaaca aggctgaagc tctgcgtctg gcacgattgc tgtatcagca
    38281 agaaccggca aatcttaccc gcctggatca actgacctgg caactgatgc agaacgggca
    38341 gtcacgcgaa gctgccgatt tattgctgca acgctatcct tttcagggcg atgcgcgtgt
    38401 cagccagacg ttaatggcgc gactggcgtc tctgctggaa agtcatcctt acctggcaac
    38461 gccggcgaag gtggcaattt tatcgaaacc tttaccgctg gcggagcaac gtcagtggca
    38521 aagtcagttg ccgggtattg ctgataattg cccggcaata gttcgcttgc tgggcgatat
    38581 gtcgccttct tacgatgctg ccgcctggaa ccgtctggca aagtgttatc gggacacgct
    38641 acccggtgtg gcgttgtatg catggcttca ggccgaacaa cgacaaccga acgcctggca
    38701 acatcgtgcg gtagcctatc aggcgtatca ggttgaggac tacgccaccg cactgacagc
    38761 ctggcagaaa atcagtcttc acgacatgag caatgaggat ctgcttgctg ccgccaatac
    38821 cgcccaggcg gcaggaaatg gcgcggctcg cgatcgctgg ttacaacagg cagaacaacg
    38881 tggactggga aacaatgccc tctactggtg gctgcatgcg caacgttaca ttccaggtca
    38941 gccggaactc gcactgaacg atctcacgcg ctcaatcaat attgcgcctt ctgccaacgc
    39001 ttacgttgcg cgggcgacaa tttatcgcca acgtcataat atcccggcgg cggtgagtga
    39061 tttgcgcgcc gcgctggaac tggaaccgaa taatagcaac acccaggcag cgcttggtta
    39121 cgccttgtgg gatagcggtg atatcgcaca gtcgcgggaa atgctcgaac aggcgcataa
    39181 agggctaccg gacgatccgg cacttatccg acaactggcc tacgtgaacc agcgtctgga
    39241 tgacatgcct gcgacgcagc actacgcccg gctggtgatt gatgacattg ataatcaggc
    39301 gctgataacc ccactgaccc cagagcaaaa tcagcaacgc ttcaatttcc gccgtctgca
    39361 tgaggaggtc ggtcgccgct ggacgttcag tttcgattct tctatcggct tgcgttccgg
    39421 cgcaatgagt accgctaaca ataatgtcgg cggcgcagcg ccagggaaaa gctatcgtag
    39481 ctacgggcaa ctggaagccg agtaccgcct cggacgcaat atgctgctgg aaggcgacct
    39541 gctctcagtt tatagccgcg tctttgccga taccggtgaa aacggggtga tgatgccggt
    39601 gaaaaatccg atgtccggca ccggtctgcg ctggaagcca ctgcgcgatc agatcttttt
    39661 cctcgccgtc gaacagcagt tgccgctgaa cggacaaaat ggcgcatccg ataccatgct
    39721 gcgcgccagc gcctcattct ttaatggcgg caaatacagc gacgaatggc acccgaacgg
    39781 ttcaggctgg tttgcccaaa acctgtacct cgatgcggcg caatatatcc gccaggatat
    39841 tcaggcgtgg acggcagatt atcgcgtcag ctggcatcag aaggtggcga acgggcagac
    39901 tattgagcct tatgctcacg ttcaggacaa cggctatcgt gataaaggca ctcagggcgc
    39961 gcagcttggc ggtgtcgggg tccgctggaa tatctggacc ggcgagacgc actacgacgc
    40021 ctggccgcac aaagtcagtc tcggcgtcga atatcaacat acctttaagg cgattaatca
    40081 acgtaacgga gagcgcaaca acgcgtttct caccattgga gtgcactggt aaatgcgtaa
    40141 gttcattttc gtattgctga cactgctttt ggtcagccct ttttcctttg cgatgaaagg
    40201 tattatctgg caaccacaaa accgtgatag tcaggttacc gatacccagt ggcaggggct
    40261 gatgagtcag ttacgtttgc aaggcttcga tacactggtt ttgcaatgga cccgttacgg
    40321 cgatgcattc acccagccag aacagcgcgc gttattgttt aagcgtgcta cagctgcgca
    40381 acaggctggc ctgaagctga ttgtcgggct gaacgccgat ccggaatttt ttatgcacca
    40441 gaaacagtcg tccgcagcgc tggaaagcta tcttaatcgc ctgctggctg ccgatctcca
    40501 gcaagccaga ttatggagcg ccgtgcctgg cgtaacgccg gatggctggt acatcagcgc
    40561 ggaaattgac gacctgaact ggcgcagcga agccgcccgt cagcctttgc taacatggtt
    40621 aaacaacgcg cagcggttga ttagcgatgt ttcagcaaaa ccggtttata tcagtagttt
    40681 tttcgccgga aacatgtcgc cagatggcta tcgccaactg ctggaacaag ttaaagcaac
    40741 cggtgttaat gtctgggtac aggatggcag cggcgtggat aaactgaccg ctgaacagcg
    40801 tgaacgttat ctacaggcca gcgccgattg ccaaagttcc gccccagcaa gcggcattgt
    40861 ttatgaactg tttgtcgctg gtaaaggtaa aacgtttaca gcgaaaccga aaccggatgc
    40921 agagatcgcc tcgctgttag cgaaacgttc ctcttgcggt aaagacacgc tctatttctc
    40981 tctgcgctat ttgcccgtcg cgcagagtat ccttgagtat taatgattaa agggagcaac
    41041 ttagcttgct cctttccttt tcactttcct aatcaaaatc ctggcaagat gaattgttta
    41101 gcagaatttc tgttttcatt ttattcgtct gacgtgttcc aattatttag caaaactgcg
    41161 acaacaaagt gatggtgtaa tttgtataag ggaacaaata aatggcgagg aaatggctca
    41221 ccctgcagga ttcgaacctg tagcccacga cttagaaact ccaggaacga cattttaagt
    41281 caacaaatta ccgcgccatc tctgcgttca cacgtcccac tacctcaaaa tatgtaaaac
    41341 cttgcaagcc attgcgaggc cttatgtgtc tcagttttgt tcctcttttt tatactaaaa
    41401 aacacagcaa ttgaggataa acctcatgct attatcgcta atatgcatct aaaggcatgg
    41461 cacttgaata gataaaaact ccacaaagaa aaaaataatt agcccataaa accacaatac
    41521 aaaacacaaa cagacaatta aactaaaaaa ctgacagcgc attgtgacaa aaatccaatg
    41581 ttaa
//
LOCUS       DAHHTG010000028        40896 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.028, whole genome shotgun sequence.
ACCESSION   DAHHTG010000028 DAHHTG010000000
VERSION     DAHHTG010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 40896)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40896)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40896
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.028"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            437..1654
                     /gene="alaA"
                     /locus_tag="NBI74_001475"
     CDS             437..1654
                     /gene="alaA"
                     /locus_tag="NBI74_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase AlaA"
                     /protein_id="HCD1931835.1"
                     /translation="MSPIEKSSKLENVCYDIRGPVLKEAKRLEEEGNKVLKLNIGNPA
                     PFGFDAPDEILVDVIRNLPTAQGYCDSKGLYSARKAIMQHYQARGMRDVTVEDIYIGN
                     GVSELIVQAMQALLNSGDEMLVPAPDYPLWTAAVSLSSGKAVHYLCDESSDWFPDLDD
                     IRAKITPRTRGIVIINPNNPTGAVYSKELLMEIVEIARQHNLIIFADEIYDKILYDDA
                     EHHSIAPLAPDLLTITFNGLSKTYRVAGFRQGWMVLNGPKKHAKGYIEGLEMLASMRL
                     CANVPAQHAIQTALGGYQSISEFITPGGRLYEQRNRAWELINDIPGVSCVKPRGALYM
                     FPKIDAKRFNIHDDQKMVLDFLLQEKVLLVQGTAFNWPWPDHFRIVTLPRVDDIELSL
                     SKFARFLSGYHQL"
     gene            1738..2337
                     /gene="yfbR"
                     /locus_tag="NBI74_001476"
     CDS             1738..2337
                     /gene="yfbR"
                     /locus_tag="NBI74_001476"
                     /EC_number="3.1.3.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-deoxynucleotidase"
                     /protein_id="HCD1931836.1"
                     /translation="MKQSHFFAHLSRLKLINRWPLMRNVRTENVSEHSLQVAMVAHAL
                     AAIKNRKFGGNVNAERIALLAMYHDASEVLTGDLPTPVKYFNSQIAQEYKAIEKIAQQ
                     KLVDMVPEELQDIFAPLIDEHAYSDEEKSLVKQADALCAYLKCLEELAAGNNEFLLAK
                     TRLEATLEARRSQEMDYFMEVFVPSFHLSLDEISQDSPL"
     gene            complement(2396..4228)
                     /locus_tag="NBI74_001477"
     CDS             complement(2396..4228)
                     /locus_tag="NBI74_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HCD1931837.1"
                     /translation="MNGELIWVLSLLAVAIVLFATGRVRMDAVALFVIVAFALSGTLT
                     VPEVFSGFSDPNVVLIAALFIIGDGLVRTGVATVMGTWLVKVAGNSEIKMLVLLMLTV
                     AGLGAFMSSTGVVAIFIPVVLSVAMRMQTSPSRLMMPLSFAGLISGMMTLVATPPNLV
                     VNSELLREGYHGFSFFSVTPIGLVVLVLGILYMLVMRFMLKGDTQTPQREGWTRRTFR
                     DLIREYRLTGRARRLAIRPGSPMIGQRLDDLKLRERYGANVIGVERWRRFRRVIVNVN
                     GVSEFRARDVLLIDMSAADVDLRQFCSEQLLEPMVLRGEYFSDQALDVGMAEISLIPE
                     SELIGKSVREIGFRTRYGLNVVGLKRNGVALEGSLADEPLLLGDIILVVGNWKLIGML
                     AKQGRDFVALNLPEEVSEASPAHSQAPHAIFCLVLMVALMLTDEIPNPVAAIIACLLM
                     GKFRCIDAESAYKSIHWPSIILIVGMMPFAVALQKTGGVALAVKGLMDIGGGYGPHMM
                     LGCLFVLSAVIGLFISNTATAVLMAPIALAAAKTMGVSPYPFAMVVAMAASAAFMTPI
                     SSPVNTLVLGPGNYSFSDFVKLGVPFTIIVMAVCVVMIPMLFPF"
     gene            complement(4315..4965)
                     /gene="hxpA"
                     /locus_tag="NBI74_001478"
     CDS             complement(4315..4965)
                     /gene="hxpA"
                     /locus_tag="NBI74_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpA"
                     /protein_id="HCD1931838.1"
                     /translation="MRCKGFLFDLDGTLVDSLPAVERAWSNWARRHGLSPEEVLAFIH
                     GKQAITSLRHFMAGKSEADIAAEFTRLEHIEATETEGITALPGAIALLNHLNKAGIPW
                     AIVTSGSMPVARARHKIAGLPAPEVFVTAERVKRGKPEPDAYLLGAQLLGLAPQECVV
                     VEDAPAGVLSGLAAGCHVIAVNAPADTPRLNEVDLVLHSLEQITVTKQPNGDVIIQ"
     gene            complement(4976..5470)
                     /locus_tag="NBI74_001479"
     CDS             complement(4976..5470)
                     /locus_tag="NBI74_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007371080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HCD1931839.1"
                     /translation="MEMTNAQRLILSNQYKMMTMLDPANAERYRRLQTIIERGYGLQM
                     RELDREFGELKEETCRTIIDIMEMYHALHVSWSNLQDQQSIDERRVTFLGFDAATEAR
                     YLGYVRFMVNVEGRYTHFDAGTHGFNAQTPMWEKYQRMLNVWHACPRQYHLSANEINQ
                     IINA"
     gene            complement(5553..6008)
                     /locus_tag="NBI74_001480"
     CDS             complement(5553..6008)
                     /locus_tag="NBI74_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF412 domain-containing protein"
                     /protein_id="HCD1931840.1"
                     /translation="MSTPDNRSVNFFSLFRRGQHYSKTWPLEKRLAPVFVENRVIKMT
                     RYAIRFMPPIAVFTLCWQIALGGQLGPAVATALFALSLPMQGLWWLGKRSVTPLPPAI
                     LNWFYEVRGKLQESGQVLAPVEGKPDYQALADTLKRAFKQLDKTFLDDL"
     gene            6346..7548
                     /gene="ackA"
                     /locus_tag="NBI74_001481"
     CDS             6346..7548
                     /gene="ackA"
                     /locus_tag="NBI74_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HCD1931841.1"
                     /translation="MSSKLVLVLNCGSSSLKFAIIDAVNGEEYLSGLAECFHLPEARI
                     KWKMDGNKQEAALGAGAAHSEALNFIVNTILAQKPELSAQLTAIGHRIVHGGEKYTSS
                     VVIDESVIQGIKDAASFAPLHNPAHLIGIEEALKSFPQLKDKNVAVFDTAFHQTMPEE
                     SYLYALPYNLYKEHGIRRYGAHGTSHFYVTQEAAKMLNKPVEELNIITCHLGNGGSVS
                     AIRNGKCVDTSMGLTPLEGLVMGTRSGDIDPAIIFHLHDTLGMSVDAINKLLTKESGL
                     LGLTEVTSDCRYVEDNYATKEDAKRAMDVYCHRLAKYIGAYTALMDGRLDAVVFTGGI
                     GENAAMVRELSLGKLGVLGFEVDHERNLAARFGKSGFINKEGTRPAVVIPTNEELVIA
                     QDASRLTA"
     gene            7623..9767
                     /gene="pta"
                     /locus_tag="NBI74_001482"
     CDS             7623..9767
                     /gene="pta"
                     /locus_tag="NBI74_001482"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HCD1931842.1"
                     /translation="MSRIIMLIPTGTSVGLTSVSLGVIRAMERKGVRLSVFKPIAQPR
                     TGGDAPDQTTTIVRANSSTTTAAEPLKMSYVEGLLSSNQKDVLMEEIIANYHANTKDA
                     EVVLVEGLVPTRKHQFAQSLNYEIAKTLNAEIVFVMSQGTDTPEQLKERIELTRNSFG
                     GAKNTNITGVIVNKLNAPVDEQGRTRPDLSEIFDDSTKAKVNNVDPAKLQESSPLPVL
                     GAVPWSFDLIATRAIDMARHLNATIINEGDINTRRVKSVTFCARSIPHMLEHFRAGSL
                     LVTSADRPDVLVAACLAAMNGVEIGALLLTGGYEMDARISKLCERAFATGLPVFMVNT
                     NTWQTSLSLQSFNLEVPVDDHERIEKVQEYVANYINADWIDSLTATSERSRRLSPPAF
                     RYQLTELARKAGKRIVLPEGDEPRTVKAAAICAERGIATCVLLGNPAEINRVAASQGV
                     ELGAGIEIVDPEVVRENYVGRLVELRKNKGMTETVAREQLEDNVVLGTLMLEQDEVDG
                     LVSGAVHTTANTIRPPLQLIKTAPGSSLVSSVFFMLLPEQVYVYGDCAINPDPTAEQL
                     AEIAIQSADSAAAFGIEPRVAMLSYSTGTSGAGSDVEKVREATRLAQEKRPDLMIDGP
                     LQYDAAVMADVAKSKAPNSPVAGRATVFIFPDLNTGNTTYKAVQRSADLISIGPMLQG
                     MRKPVNDLSRGALVDDIVYTIALTAIQSAQQQ"
     gene            9957..11477
                     /gene="yfcC"
                     /locus_tag="NBI74_001483"
     CDS             9957..11477
                     /gene="yfcC"
                     /locus_tag="NBI74_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HCD1931843.1"
                     /translation="MSAITESKPTRRWAMPDTLVIIFFVAILTSLATWVVPVGMFDSQ
                     EVQYQVDGQTKTRKVVDPHSFRILTNEAGEPEYHRVQLFTTGDERPGLMNFPFEGLTS
                     GSKYGTAVGIIMFMLVIGGAFGIVMRTGTIDNGILALIRHTRGNEILFIPALFILFSL
                     GGAIFGMGEEAVAFAIIIAPLMVRLGYDSITTVLVTYIATQIGFASSWMNPFCVVVAQ
                     GIAGVPVLSGSGLRIVVWVIATLIGLIFTMVYASRVKKNPLLSRVHESDRFFREKQAD
                     VEQRPFTFGDWLVLIVLTAVMVWVIWGVIVNAWFIPEIASQFFTMGLVIGIIGVVFRL
                     NGMTVNTMASSFTEGARMMIAPALLVGFAKGILLLVGNGEAGDASVLNTILNSIANAI
                     SGLDNAVAAWFMLLFQAVFNFFVTSGSGQAALTMPLLAPLGDLVGVNRQVTVLAFQFG
                     DGFSHIIYPTSASLMATLGVCRVDFRNWLKVGATLLGLLFIMSSVVVIGAQLMGYH"
     gene            complement(11510..12052)
                     /gene="yfcD"
                     /locus_tag="NBI74_001484"
     CDS             complement(11510..12052)
                     /gene="yfcD"
                     /locus_tag="NBI74_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase YfcD"
                     /protein_id="HCD1931844.1"
                     /translation="MEQRRLASTEWVDIVNEENEVIAQASREQMRAQCLRHRATYIVV
                     HDGMGKILVQRRTETKDFLPGMLDATAGGVVQADEQLLESARREAEEELGIAGVPFAE
                     HGQFYFEDKNCRVWGALFSCVSHGPFALQEDEVSEVCWLTPEEITARCDEFTPDSLKA
                     LALWMKRNAKNEAVETETAE"
     gene            complement(12110..12661)
                     /gene="yfcE"
                     /locus_tag="NBI74_001485"
     CDS             complement(12110..12661)
                     /gene="yfcE"
                     /locus_tag="NBI74_001485"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311211.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase"
                     /protein_id="HCD1931845.1"
                     /translation="MKLMFASDIHGSLPATERVLELFAQSGAQWLVILGDVLNHGPRN
                     ALPEGYAPAKVAERLNEVAHKVIAVRGNCDSEVDQMLLHFPITAPWQQVLLEKQRLFL
                     THGHLFGPENLPALNQNDVLVYGHTHLPVAEQRGEIFHFNPGSVSIPKGGNPASYGML
                     DNDVLSVIALNDQSIIAQVAINP"
     gene            complement(12717..13361)
                     /gene="yfcF"
                     /locus_tag="NBI74_001486"
     CDS             complement(12717..13361)
                     /gene="yfcF"
                     /locus_tag="NBI74_001486"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase"
                     /protein_id="HCD1931846.1"
                     /translation="MSKPAITLWSDAHFFSPYVLSAWVALQEKGLSFHIKTIDLDSGE
                     HLQPTWQGYGQTRRVPLLQIDDFELSESSAIAEYLEDRFAPPTWERIYPLDLENRARA
                     RQIQAWLRSDLMPIREERPTDVVFAGAKKAPLTAEGKASAEKLFAMAEHLLALGQPNL
                     FGEWCIADTDLALMINRLVLHGDEVPERLVDYATFQWQRASVQRFIALSAKQSG"
     gene            13497..14144
                     /gene="yfcG"
                     /locus_tag="NBI74_001487"
     CDS             13497..14144
                     /gene="yfcG"
                     /locus_tag="NBI74_001487"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GSH-dependent disulfide bond oxidoreductase"
                     /protein_id="HCD1931847.1"
                     /translation="MIDLYFAPTPNGHKITLFLEEAGLDYRLIKVDLGKGGQFRPEFL
                     RISPNNKIPAIVDHSPADGGEPLSLFESGAILLYLAEKTGLFLNHETRERAVTLQWLF
                     WQVGGLGPMLGQNHHFNHAAPQTIPYAIERYQVETQRLYHVLNKRLENSPWLGGENYS
                     IADIACWPWVNAWTRQRIDLAMYPAVKNWHERIRSRPATGQALLKAQHGDERSDS"
     gene            14201..14563
                     /gene="folX"
                     /locus_tag="NBI74_001488"
     CDS             14201..14563
                     /gene="folX"
                     /locus_tag="NBI74_001488"
                     /EC_number="5.1.99.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate 2'-epimerase"
                     /protein_id="HCD1931848.1"
                     /translation="MAQPAAIIRIKNLRLRTFIGIKEEEINNRQDIVINVTIHYPADK
                     ARTSEDINDALNYRTVTKNIIQHVENNRFSLLEKLTQDVLDIAREHHWVTYAEVEIDK
                     LHALRYTDSVSMTLSWQR"
     gene            14584..15477
                     /locus_tag="NBI74_001489"
     CDS             14584..15477
                     /locus_tag="NBI74_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HCD1931849.1"
                     /translation="MNIVITGGTGLIGRHLIPRLLDLGHQITVVTRNPQKASSVLGPR
                     VTLWQGLAEQSNLNGVDAVINLAGEPIADKRWTHERKERLCQSRWNTTQKLVDLINAS
                     DTPPSVLISGSATGYYGDLGEVVVTEEEPPHNEFTHKLCARWEEIACRAQSDKTRVCL
                     LRTGVVLAPDGGILGKMLPPFRFGLGGPIGSGRQYLAWIHIDDMVNGILWLLDNELRG
                     PFNMVSPYPVRNEQFAHALGHALHRPTILRVPATAIRLLMGESSVLVLGGQRALPKKL
                     EEAGFAFRWYDLEEALADVVR"
     gene            complement(15636..16409)
                     /gene="hisP"
                     /locus_tag="NBI74_001490"
     CDS             complement(15636..16409)
                     /gene="hisP"
                     /locus_tag="NBI74_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter ATP-binding protein
                     HisP"
                     /protein_id="HCD1931850.1"
                     /translation="MSENKLNVIDLHKRYGEHEVLKGVSLQANAGDVISIIGSSGSGK
                     STFLRCINFLEKPSDGSIVVNGQTINLVRDKDGQLKVADKNQLRLLRTRLTMVFQHFN
                     LWSHMTVLENVMEAPIQVLGLSKQEARERAVKYLAKVGIDERAQGKYPVHLSGGQQQR
                     VSIARALAMEPEVLLFDEPTSALDPELVGEVLRIMQQLAEEGKTMVVVTHEMGFARHV
                     STHVIFLHQGKIEEEGAPEQLFGNPQSPRLQQFLKGSLK"
     gene            complement(16417..17133)
                     /locus_tag="NBI74_001491"
     CDS             complement(16417..17133)
                     /locus_tag="NBI74_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCD1931851.1"
                     /translation="MIEILHEYWKPLLWTDGYRFTGVAITLWLLILSVVIGGVLALFL
                     AIGRVSSNKYIQFPIWLFTYIFRGTPLYVQLLVFYSGMYTLEIVKGTEFLNAFFRSGL
                     NCTVLALTLNTCAYTTEIFAGAIRSVPHGEIEAARAYGFSTFKMYRCIILPSALRIAL
                     PAYSNEVILMLHSTALAFTATVPDLLKIARDINAATYQPFTAFGIAAVLYLIISYVLI
                     SLFRRAEKRWLQHVKPSSTH"
     gene            complement(17130..17816)
                     /gene="hisQ"
                     /locus_tag="NBI74_001492"
     CDS             complement(17130..17816)
                     /gene="hisQ"
                     /locus_tag="NBI74_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012124147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter permease HisQ"
                     /protein_id="HCD1931852.1"
                     /translation="MLYGFSSVILQGALVTLELAISSVVLAVIIGLIGAGGKLSQNRL
                     SGLIFEGYTTLIRGVPDLVLMLLIFYGLQIALNTVTEAMGVGQIDIDPMVAGIITLGF
                     IYGAYFTETFRGAFMAVPKGHIEAATAFGFTRGQVFRRIMFPAMMRYALPGIGNNWQV
                     ILKSTALVSLLGLEDVVKATQLAGKSTWEPFYFAIVCGVIYLVFTTVSNGVLLFLERR
                     YSVGVKRADL"
     gene            complement(17906..18688)
                     /gene="hisJ"
                     /locus_tag="NBI74_001493"
     CDS             complement(17906..18688)
                     /gene="hisJ"
                     /locus_tag="NBI74_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter substrate-binding
                     protein HisJ"
                     /protein_id="HCD1931853.1"
                     /translation="MKKLVLSLSLVLAFSSATAAFAAIPQNIRIGTDPTYAPFESKNS
                     QGELVGFDIDLAKELCKRINTQCTFVENPLDALIPSLKAKKIDAIMSSLSITEKRQQE
                     IAFTDKLYAADSRLVVAKNSDIQPTVESLKGKRVGVLQGTTQETFGNEHWAPKGIEIV
                     SYQGQDNIYSDLTAGRIDAAFQDEVAASEGFLKQPVGKDYKFGGPSVKDEKLFGVGTG
                     MGLRKEDNELREALNKAFAEMRADGTYEKLAKKYFDFDVYGG"
     gene            complement(18909..19691)
                     /gene="argT"
                     /locus_tag="NBI74_001494"
     CDS             complement(18909..19691)
                     /gene="argT"
                     /locus_tag="NBI74_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine/arginine/ornithine ABC transporter
                     substrate-binding protein ArgT"
                     /protein_id="HCD1931854.1"
                     /translation="MKKSILALSLLVGLSAAASSYAALPETVRIGTDTTYAPFSSKDA
                     KGDFVGFDIDLGNEMCKRMQVKCTWVASDFDALIPSLKAKKIDAIISSLSITDKRQQE
                     IAFSDKLYAADSRLIAAKGSPIQPTLDSLKGKHVGVLQGSTQEAYANETWRSKGVDVV
                     AYANQDLVYSDLAAGRLDAALQDEVAASEGFLKQPAGKDFAFAGPSVKDKKYFGDGTG
                     VGLRKDDAELTAAFNKALGELRQDGTYDKMAKKYFDFNVYGD"
     gene            complement(19957..20526)
                     /gene="ubiX"
                     /locus_tag="NBI74_001495"
     CDS             complement(19957..20526)
                     /gene="ubiX"
                     /locus_tag="NBI74_001495"
                     /EC_number="2.5.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin prenyltransferase UbiX"
                     /protein_id="HCD1931855.1"
                     /translation="MKRLIVGISGASGAIYGVRLLQVLRDVTDIETHLVMSQAARQTL
                     SLETDFSLREVQALADVTHDARDIAASISSGSFQTLGMVILPCSIKTLSGIVHSYTDG
                     LLTRAADVVLKERRPLVLCVRETPLHLGHLRLMTQAAEIGAVIMPPVPAFYHRPQSLD
                     DVINQTVNRVLDQFAVTLPEDLFARWQGA"
     gene            complement(20621..22138)
                     /gene="purF"
                     /locus_tag="NBI74_001496"
     CDS             complement(20621..22138)
                     /gene="purF"
                     /locus_tag="NBI74_001496"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HCD1931856.1"
                     /translation="MCGIVGIAGVMPVNQSIYDALTVLQHRGQDAAGIITIDANNCFR
                     LRKANGLVSDVFEARHMQRLQGNMGIGHVRYPTAGSSSASEAQPFYVNSPYGITLAHN
                     GNLTNAHELRKKLFEEKRRHINTTSDSEILLNIFASELDNFRHYPLEADNIFAAIAAT
                     NRLIRGAYACVVMIIGHGMVAFRDPNGIRPLVLGKRDIDDNRTEYMVASESVALDTLG
                     FEFLRDVAPGEAIYITEEGQLFTRQCADNPVSNPCLFEYVYFARPDSFIDKISVYSAR
                     VNMGTKLGEKIAREWEDLDIDVVIPIPETSCDIALEIARILGKPYRQGFVKNRYVGRT
                     FIMPGQQLRRKSVRRKLNANRAEFRDKNVLLVDDSIVRGTTSEQIIEMAREAGAKKVY
                     LASAAPEIRFPNVYGIDMPSATELIAHGREVDEIRQIIGADGLIFQDLNDLIEAVRAE
                     NPDIQQFECSVFNGVYVTKDVDQGYLDFLDTLRNDDAKAVQRQNEVENLEMHNEG"
     gene            complement(22175..22663)
                     /gene="cvpA"
                     /locus_tag="NBI74_001497"
     CDS             complement(22175..22663)
                     /gene="cvpA"
                     /locus_tag="NBI74_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin V production protein"
                     /protein_id="HCD1931857.1"
                     /translation="MVWIDYAIIAVIAFSSLVSLIRGFVREALSLVTWGCAFFVASHY
                     YTYLSVWFTGFEDELVRNGIAIAVLFIATLIVGAIVNFVIGQLVEKTGLSGTDRVLGV
                     CFGALRGVLIVAAILFFLDSFTGVSKSEDWSKSQLIPQFSFIIRWFFDYLQSSSSFLP
                     RA"
     gene            complement(23104..23766)
                     /gene="dedD"
                     /locus_tag="NBI74_001498"
     CDS             complement(23104..23766)
                     /gene="dedD"
                     /locus_tag="NBI74_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DedD"
                     /protein_id="HCD1931858.1"
                     /translation="MASKFQNRLVGTIVLVALGVIVLPGLLDGQKKHYQDEFAAIPLV
                     PKAGDRDEPDMMPAATQALPTQPPEGAAEEVRAGDAAAPSLDPATIAANNTEFEPEPA
                     PVVPPKPKPVESPKPKVEAPPAPKPEPKPVVEEKAAPTGKAYVVQLGALKNADKVNEI
                     VGKLRGAGYRVYTSPSTPVQGKITRILVGPDASKEKLKGSLGELKQLSGLSGVVMGYT
                     PN"
     gene            complement(23756..25024)
                     /gene="folC"
                     /locus_tag="NBI74_001499"
     CDS             complement(23756..25024)
                     /gene="folC"
                     /locus_tag="NBI74_001499"
                     /EC_number="6.3.2.12"
                     /EC_number="6.3.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tetrahydrofolate
                     synthase/dihydrofolate synthase"
                     /protein_id="HCD1931859.1"
                     /translation="MIIKRTPQAASPLASWLSYLENLHSKTIDLGLERVSQVAARLGV
                     LKPAPFVFTVAGTNGKGTTCRTLESILMAAGYKVGVYSSPHLVRYTERVRVQGQELPE
                     SAHTASFAEIESARGDISLTYFEYGTLSALWLFKQAQLDVVILEVGLGGRLDATNIVD
                     ADVAVVTSIALDHTDWLGPDRESIGREKAGIFRSEKPAIVGEPEMPSTIADVGQEKGA
                     LLQRRGVEWNYSVTDHDWTFSDAHGTLENLPLPLVPQPNAATALAALRASGLEVSENA
                     IRDGIASAILPGRFQIVSESPRVIFDVAHNPHAAEYLTGRMKALPKNGRVLAVIGMLH
                     DKDIAGTLAWLKSVVDDWYCAPLEGPRGATAEQLLEHLGNGKSFDSVAQAWDAAMADA
                     KAEDTVLVCGSFHTVAHVMEVIDARRSGGK"
     gene            complement(25094..26008)
                     /gene="accD"
                     /locus_tag="NBI74_001500"
     CDS             complement(25094..26008)
                     /gene="accD"
                     /locus_tag="NBI74_001500"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HCD1931860.1"
                     /translation="MSWIERIKSNITPTRKASIPEGVWTKCDSCGQVLYRAELERNLE
                     VCPKCDHHMRMTARNRLHSLLDEGSLVELGSELEPKDVLKFRDSKKYKDRLASAQKET
                     GEKDALVVMKGTLYGMPVVAAAFEFAFMGGSMGSVVGARFVRAVEQALEDNCPLICFS
                     ASGGARMQEALMSLMQMAKTSAALAKMQERGLPYISVLTDPTMGGVSASFAMLGDLNI
                     AEPKALIGFAGPRVIEQTVREKLPPGFQRSEFLIEKGAIDMIVRRPEMRLKLASILAK
                     LMNLPAPNPEAPREGVVVPPVPDQEPEA"
     gene            complement(26164..26823)
                     /locus_tag="NBI74_001501"
     CDS             complement(26164..26823)
                     /locus_tag="NBI74_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCD1931861.1"
                     /translation="MDLIYFLIDFILHIDVHLAELVAEYGVWVYAILFLILFCETGLV
                     VTPFLPGDSLLFVAGALASLETNDLNVHMMVVLMLIAAIVGDAVNYTIGRLFGEKLFS
                     NPNSKIFRRSYLDKTHQFYEKHGGKTIILARFVPIVRTFAPFVAGMGHMSYRHFAAYN
                     VIGALLWVLLFTYAGYFFGTLPFIQSNLKLMIVGIIFVSILPGVFEFIRHKRAAARAA
                     K"
     gene            complement(26906..27718)
                     /gene="truA"
                     /locus_tag="NBI74_001502"
     CDS             complement(26906..27718)
                     /gene="truA"
                     /locus_tag="NBI74_001502"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HCD1931862.1"
                     /translation="MSDQQQPPVYKIALGIEYDGSRYYGWQRQNEVRSVQEKLEKALS
                     QVANEPITVFCAGRTDAGVHGTGQVVHFETTAQRKDAAWTLGVNANLPGDIAVRWVKA
                     VPDDFHARFSATARRYRYIIYNHRLRPAVLSKGVTHFYEPLDAERMHRAAQCLLGEND
                     FTSFRAVQCQSRTPWRNVMHINVTRHGPYVVVDIKANAFVHHMVRNIVGSLMEVGAHN
                     QPESWIAELLAAKDRTLAAATAKAEGLYLVAVDYPDRYDLPKPPMGPLFLAD"
     gene            complement(27718..28731)
                     /locus_tag="NBI74_001503"
     CDS             complement(27718..28731)
                     /locus_tag="NBI74_001503"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HCD1931863.1"
                     /translation="MSEGWNIAVLGATGAVGEALLETLAERQFPVGEIYALARNESAG
                     EQLRFGGKTITVQDAAEFDWTQAQLAFFVAGKEATAAWVEEATNSGCLVIDSSGLFAL
                     EPDVPLVVPEVNPFVLTDYRNRNVIAVPDSLTSQLLAALKPLIDQGGLSRISVTSLIS
                     ASAQGKKAVDALAGQSAKLLNGIPIDEEDFFGRQLAFNMLPLLPDSEGSVREERRIVD
                     EVRKILQDEGLMISASVVQAPVFYGHAQMVNFEALRPLAAEEARDAFAQGEDIVLSEE
                     NEFPTQVGDASGTPHLSVGCVRNDYGMPEQVQFWSVADNVRFGGALMAVKIAEKLVQE
                     YLY"
     gene            complement(28797..29933)
                     /gene="pdxB"
                     /locus_tag="NBI74_001504"
     CDS             complement(28797..29933)
                     /gene="pdxB"
                     /locus_tag="NBI74_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-phosphoerythronate dehydrogenase PdxB"
                     /protein_id="HCD1931864.1"
                     /translation="MKILVDENMPYARDLFSRLGEVTAVPGRPIPVAQLADADALMVR
                     SVTKVNESLLAGKPIKFVGTATAGTDHVDEAWLKQAGIGFSAAPGCNAIAVVEYVFSS
                     LLMLAERDGFSLHDRTVGIVGVGNVGRRLQARLEALGITTLLCDPPRADRGDEGDFRS
                     LDELVQHADILTFHTPLFKDGPYKTLHLADEKLIRSLKPGAILINACRGAVVDNTALL
                     TCLNEGQKLSVVLDVWEGEPELNVELLKKVDIGTPHIAGYTLEGKARGTTQVFEAYSK
                     LIGHEQHVALDTLLPAPEFGRITLHGPLDQPTLKRLVHLVYDVRRDDAPLRKVAGIPG
                     EFDKLRKNYLERREWSSLYVICDDASAASLLCKLGFNAVHHPAR"
     gene            30032..31027
                     /gene="flk"
                     /locus_tag="NBI74_001505"
     CDS             30032..31027
                     /gene="flk"
                     /locus_tag="NBI74_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulator Flk"
                     /protein_id="HCD1931865.1"
                     /translation="MIQPISGPPPGQPPGQGDNLPSGAGNQPLSSQQRTSLESLMTKV
                     TSLTQQQRAELWAGIRHDIGLSGDSPLLSRHFPAAEHNLAQRLLAAQKSHSARQLLAQ
                     LGEYLRLGNNRQAVTDYIRHNFGQTPLNQLSPEQLKTILTLLQEGKMVIPQPQQREAT
                     DRPLLPAEHNALKQLVTKLAAATGEPSKQIWQSMLELSGVKDGELIPAKLFNHLVTWL
                     QARQTLSQQNTPTLESLQMALKQPLDASELAALSAYIQQKYGLSAQSSLSSAQAEDIL
                     NQLYQRRVKGIEPRDMQPLLNPFPPMMDTLQNMATRPALWILLVAIILMLVWLVR"
     gene            complement(31024..32202)
                     /locus_tag="NBI74_001506"
     CDS             complement(31024..32202)
                     /locus_tag="NBI74_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose transporter"
                     /protein_id="HCD1931866.1"
                     /translation="MTAVSQTETRSSANFSLFRIAFAVFLTYMTVGLPLPVIPLFVHH
                     ELGYGNTMVGIAVGIQFLATVLTRGYAGRLADQYGAKRSALQGMLACGLAGGALLLAA
                     ILPVSAPFKFALLVVGRLILGFGESQLLTGALTWGLGIVGPKHSGKVMSWNGMAIYGA
                     LAVGAPLGLLIHSHYGFAALALTTMALPLLAWACNGTVRKVPALAGERPSLWSVVGLI
                     WKPGLGLALQGVGFAVIGTFVSLYFASKGWAMAGFTLTAFGGAFVVMRVMFGWMPDRF
                     GGVKVAIVSLLVETVGLLLLWQAPGAWVALAGAALTGAGCSLIFPALGVEVVKRVPSQ
                     VRGTALGGYAAFQDIALGVSGPLAGMLATTFGYSSVFLAGAISAVLGIIVTILSFRRG
                     "
     gene            complement(32467..33687)
                     /gene="fabB"
                     /locus_tag="NBI74_001507"
     CDS             complement(32467..33687)
                     /gene="fabB"
                     /locus_tag="NBI74_001507"
                     /EC_number="2.3.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase I"
                     /protein_id="HCD1931867.1"
                     /translation="MKRAVITGLGIVSSIGNNQQEVLASLREGRSGITFSQELKDSGM
                     RSHVWGNVKLDTTGLIDRKVVRFMSDASIYAFLSMEQAIADAGLSPEAYQNNPRVGLI
                     AGSGGGSPRFQVFGADAMRGPRGLKAVGPYVVTKAMASGVSACLATPFKIHGVNYSIS
                     SACATSAHCIGNAVEQIQLGKQDIVFAGGGEELCWEMACEFDAMGALSTKYNDTPEKA
                     SRTYDAHRDGFVIAGGGGMVVVEELEHALARGAHIYAEIVGYGATSDGADMVAPSGEG
                     AVRCMKMAMHGVDTPIDYLNSHGTSTPVGDVKELAAIREVFGDKSPAISATKAMTGHS
                     LGAAGVQEAIYSLLMLEHGFIAPSINIEELDEQAAGLNIVTETTDRELTTVMSNSFGF
                     GGTNATLVMRKLKD"
     gene            33846..35852
                     /gene="mnmC"
                     /locus_tag="NBI74_001508"
     CDS             33846..35852
                     /gene="mnmC"
                     /locus_tag="NBI74_001508"
                     /EC_number="1.5.-.-"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (5-methylaminomethyl-2-thiouridine)(34)-methyltransferase
                     MnmD/FAD-dependent
                     5-carboxymethylaminomethyl-2-thiouridine(34)
                     oxidoreductase MnmC"
                     /protein_id="HCD1931868.1"
                     /translation="MKHYSIQPANLEFNAEGTPVSRDFDDVYFSNDNGLEETRYVFLG
                     GNHLEARFPEHPHPLFVVAESGFGTGLNFLTLWQAFDQFREAYPQAQLQRLHFISFEK
                     FPLARADLALAHQHWPELAPWAEQLQAQWPLPLPGCHRLLLDEGRITLDLWFGDINEL
                     TSQLDDSLNQKVDAWFLDGFAPAKNPDMWTQNLFNAMARLARPGGTLATFTSAGFVRR
                     GLQEAGFTMQKRKGFGRKREMLCGVMELTLPLPCSTPWFNRTGSSKREAAIIGGGIAS
                     ALLSLALLRRGWQVTLYCADEAPALGASGNRQGALYPLLSKHDEALNRFFSNAFTFAR
                     RFYDLLPVKFDHDWCGVTQLGWDEKSQHKIAQMLSMDLPTELAVAVEANAVEQITGVA
                     TNCSGITYPQGGWLCPAELTRNVLELAQQQGLQIHYQYQLQDLSRKDDGWLLNFAGDQ
                     QATHSLVVLANGHQISRFSQTSSLPVYSVAGQVSHIPTTPELAKLKQVLCYDGYLTPQ
                     NPANQHHCIGASYHRGSEETAYSDEDQQQNRQRLIDCFPQVQWAKEVDVSGKEARCGV
                     RCATRDHLPMVGNVPDYDATLVEYASLAEKKDEAVSAPVYDDLFMFAALGSRGLCSAP
                     LCAEILAAQMSEEPIPMDASTLAALNPNRLWVRKLLKGKAVKAG"
     gene            complement(35973..36251)
                     /locus_tag="NBI74_001509"
     CDS             complement(35973..36251)
                     /locus_tag="NBI74_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcL family protein"
                     /protein_id="HCD1931869.1"
                     /translation="MIAEFESRILALIDGMVDHASDDELFASGYLRGHLTLAIAELES
                     GDDHSAQAVHTTVSQSLEKAIGAGELSPRDQALVTDMWEHLFQQASQQ"
     gene            complement(36285..36833)
                     /gene="epmC"
                     /locus_tag="NBI74_001510"
     CDS             complement(36285..36833)
                     /gene="epmC"
                     /locus_tag="NBI74_001510"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P hydroxylase"
                     /protein_id="HCD1931870.1"
                     /translation="MNSTHHYEQLIEIFNSCFADEFNTRLIKGDDEPIYLPADAEVPY
                     NRIVFAHGFYASAIHEISHWCIAGKARRELVDFGYWYCPDGRDAQTQSQFEDVEVKPQ
                     ALDWLFCVAAGYPFNVSCDNLEGDFEPDRVVFQRRVHAQVMDYLANGIPERPARFIKA
                     LQNYYHTPELTAEQFPWPEALN"
     gene            complement(36833..37642)
                     /locus_tag="NBI74_001511"
     CDS             complement(36833..37642)
                     /locus_tag="NBI74_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HCD1931871.1"
                     /translation="METFNSLFMVSPLLLGVLFFVAMLAGFIDSIAGGGGLLTIPALM
                     AAGMSPANALATNKLQACGGSISATIYFIRRKVVSLSDQKLNIAMTFVGSMSGALLVQ
                     YVQADVLRQILPILVICIGLYFLLMPKLGEEDRQRRMYGLPFALVAGGCVGFYDGFFG
                     PAAGSFYALAFVTLCGFNLAKATAHAKLLNATSNIGGLLLFILGGKVIWATGFVMLVG
                     QFLGARMGSRLVLSKGQKLIRPMIVIVSAVMSAKLLYDSHGQEILHWLGMN"
     gene            complement(37642..38466)
                     /gene="mepA"
                     /locus_tag="NBI74_001512"
     CDS             complement(37642..38466)
                     /gene="mepA"
                     /locus_tag="NBI74_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-insensitive murein endopeptidase"
                     /protein_id="HCD1931872.1"
                     /translation="MNKTAIALLALLASSASLAATPWQKITQPVPGNAQSIGSFSNGC
                     IIGADTLPIQSEHYQVMRTDQRRYFGHPDLVMFIQRLSRQVSNLGMGTVLIGDMGMPA
                     GGRFNGGHASHQTGLDVDIFLQLPKTRWTSAQLLRPQALDLVSRDGKHVVPTLWKPEI
                     FSLIKLAAQDKDVTRIFVNPAIKQQLCLDAGTDRDWLRKVRPWFQHRAHMHVRLRCPA
                     DSLECEDQPLPPPGDGCGAELQSWFEPPKPGTTKPEKKTPPPLPPSCQALLDEHVI"
     gene            complement(38470..39555)
                     /gene="aroC"
                     /locus_tag="NBI74_001513"
     CDS             complement(38470..39555)
                     /gene="aroC"
                     /locus_tag="NBI74_001513"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HCD1931873.1"
                     /translation="MAGNTIGQLFRVTTFGESHGLALGCIVDGVPPGIPLTEADLQHD
                     LDRRRPGTSRYTTQRREPDQVKILSGVFEGVTTGTSIGLLIENTDQRSQDYSAIKDVF
                     RPGHADYTYEQKYGLRDYRGGGRSSARETAMRVAAGAIAKKYLAEKFGIEIRGCLTQM
                     GDIPLEIKDWSQVEQNPFFCPDPDKIDALDELMRALKKEGDSIGAKVTVVASGVPAGL
                     GEPVFDRLDADIAHALMSINAVKGVEIGDGFDVVALRGSQNRDEITKDGFQSNHAGGI
                     LGGISSGQQIIAHMALKPTSSITVPGRTINRFGEEVEMITKGRHDPCVGIRAVPIAEA
                     MLAIVLMDHLLRQRAQNADVKTEIPRW"
     gene            complement(39590..40522)
                     /gene="prmB"
                     /locus_tag="NBI74_001514"
     CDS             complement(39590..40522)
                     /gene="prmB"
                     /locus_tag="NBI74_001514"
                     /EC_number="2.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HCD1931874.1"
                     /translation="MDKIFVDEAVNELQTIQDMLRWSVSRFSAANIWYGHGTDNPWDE
                     AVQLVLPSLYLPLDIPEDMRTARLTSSEKHRIVERVIRRVNERIPVAYLTNRAWFCGH
                     EFYVDERVLVPRSPIGELINNKFAGLISKQPQHILDMCTGSGCIAIACAYAFPEAEVD
                     AVDISPDALAVAEQNIEEHGLIHNVIPIRSDLFRDLPKVQYDLIVTNPPYVDAEDMSD
                     LPNEYRHEPELGLASGTDGLKLTRRILGNAADYLADDGVLICEVGNSMVHLMEQYPDV
                     PFTWLEFDNGGDGVFMLTKEQLIAAREHFAIYKD"
     gene            40688..>40896
                     /locus_tag="NBI74_001515"
     CDS             40688..>40896
                     /locus_tag="NBI74_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1931875.1"
                     /translation="MKKKTTLSEEDQALFRQLMAGTRKIKQDTIVHRPQRKKISEVPV
                     KRLIQEQADASHYFSDEFQPLLNTEG"
BASE COUNT        10081 a        11125 c        10221 g         9469 t
ORIGIN      
        1 aacctttttg aatattaaat aataattaat tagcttcatc cccattcatt aattccgctt
       61 aacaatagtt tcacaccttg cccccattgt gacacctcgg aaatcatcac gctgttaaac
      121 tctccgtaat gcggaaatat catcatcgcg attcagtagt gaattcttaa acgaggcatt
      181 ttcacacaat tatcttacag ataaaaaaac cacacttaca attaataatc agaacaattc
      241 actacataac attacttatt aagcatatgc acctcattat tttgccatta ttaagttatt
      301 aacagcacaa tcgagccttc ccctctggca aaatcttatt ctgcagacct tcaaaacacc
      361 gtcctggggg agtacattgt tctaagctga cttccacggc agggagtggc gataacagca
      421 aaaaaggtca agattcatgt cccccattga aaaatccagc aaattagaga atgtctgtta
      481 tgacatccgt ggtccggtgc tgaaagaagc aaaacgcctg gaagaagaag gtaacaaggt
      541 tctgaaactg aacatcggca accctgcccc gttcggtttt gacgcgccag atgaaattct
      601 cgttgacgtg atacgcaacc tgcctaccgc tcaagggtat tgcgattcca aaggtcttta
      661 ctccgcgcgt aaagccatca tgcagcacta ccaggctcgt ggcatgcgtg atgttaccgt
      721 ggaagatatt tacatcggca atggtgtatc ggagcttatc gttcaggcaa tgcaagcatt
      781 gctgaacagc ggggacgaaa tgttggttcc tgcacctgat tacccactat ggaccgcggc
      841 ggtttcactc tccagcggta aagcggtgca ttatctttgc gatgaatcct ctgactggtt
      901 cccggacctc gatgatattc gcgctaaaat tacgcctcgt acgcgtggga tcgttattat
      961 caacccaaat aacccaaccg gcgcggtata ttccaaagag cttttaatgg agattgtgga
     1021 gattgcacgt cagcataatc tcattatctt tgccgatgaa atttatgaca aaattctcta
     1081 cgacgacgct gagcatcact ccattgcgcc gcttgcacct gacctgctga ccattacctt
     1141 taacggactg tcgaaaacgt accgcgttgc aggcttccgt caggggtgga tggtgttgaa
     1201 cgggccgaaa aaacacgcta aaggttacat cgaaggtctg gaaatgctgg cttcaatgcg
     1261 cctgtgtgct aacgttcctg cgcaacacgc cattcagacc gcactgggtg gttatcagag
     1321 catcagtgaa tttattaccc ctggcggtcg tctttatgag cagcgtaacc gcgcgtggga
     1381 attgatcaac gatattccgg gcgtttcctg cgtgaaacct cgtggtgcgc tgtatatgtt
     1441 cccgaaaatc gacgccaaac gctttaacat tcacgacgat cagaaaatgg tgctggattt
     1501 cctgttgcag gaaaaagtcc tgttggtgca agggacggca ttcaactggc cgtggccgga
     1561 tcacttccgc attgtcacgc taccgcgtgt cgatgatatc gagctgtctt tgagtaagtt
     1621 cgcgcgtttc ctttctggtt atcatcagct gtaatcttaa tttcactgcc ggagattgca
     1681 tccggcagtg ttatcccgcc acaatgacct gatgatgtca tcatacgtaa ggtcactatg
     1741 aaacagagcc atttttttgc ccatctctcc cgcctgaaac tcattaaccg ctggccgcta
     1801 atgcgcaacg tgcggacgga aaatgtgtcc gaacacagtt tgcaggtagc gatggtcgcc
     1861 catgcgctgg cagctatcaa aaatcgtaaa tttggcggta atgtcaacgc cgaacgtatc
     1921 gctttactgg cgatgtacca cgatgcttca gaagtgctca ccggagatct ccctactccg
     1981 gtgaaatact tcaattcgca aatcgctcag gaatacaagg ctattgaaaa aatcgctcag
     2041 caaaaactgg tcgatatggt tccggaagag ctacaggata tctttgcgcc gttaattgac
     2101 gagcatgcat atagcgatga agaaaaatcg ctggtgaaac aggcagatgc actgtgtgca
     2161 tatctgaaat gtctggaaga actcgcggcc ggaaataatg aattcttgct ggcaaaaacg
     2221 cgactggaag cgacgcttga agcgcgtcgc agccaggaga tggactactt catggaagta
     2281 tttgttccca gcttccatct ttcgctcgat gagattagcc aggattcacc gctgtaagca
     2341 gctggagtct gcgtcgcatc aggcaataag cgccggatgc gacatcaggc tcttgtcaaa
     2401 acggaaacag catcgggatc atcaccacac aaaccgccat cacgataatg gtgaacggta
     2461 cccccaactt aacaaagtca ctgaagctgt aattccccgg acctaaaacc agtgtgttaa
     2521 caggtgaaga tatcggtgtc ataaaggcgg cggatgctgc catcgccacg accatcgcaa
     2581 atggataagg cgacaccccc atcgttttgg cagcagccag cgcaatcgga gccatcaaca
     2641 ccgccgtcgc agtattagaa ataaatagcc caataaccgc cgacaagaca aacaaacaac
     2701 ccagcatcat atgtggccca taaccgccgc caatgtccat cagccctttc accgccagcg
     2761 cgacacctcc cgttttctgt aatgccacag caaacggcat catcccaacg atcaaaataa
     2821 tgctcggcca gtgaatggat ttataggcac tttcggcatc gatacagcgg aatttcccca
     2881 tcagcaggca ggcgatgata gcggcaacag gattaggaat ttcatcggtc agcattaacg
     2941 ccaccattag caccagacag aaaatggcat ggggtgcctg gctgtgcgcg ggcgatgctt
     3001 cactcacctc ttccggtaag ttcagcgcca cgaagtcgcg gccctgtttg gccagcatac
     3061 cgatcagttt ccagttacca acaaccagaa tgatatcgcc cagcagcaga ggctcatccg
     3121 ccagcgaacc ttccagcgcc acaccattgc gctttagccc caccacattc aatccgtagc
     3181 gggtacgaaa accaatttcg cgcaccgatt taccaataag ttctgactca ggaattaatg
     3241 aaatctctgc catgcccaca tcaagggcct ggtcagaaaa atactcgccg cgcagtacca
     3301 tcggctccag caattgctca ctacaaaatt gccggagatc gacatcagcc gcagacatat
     3361 caataagcaa aacgtcacgc gcgcgaaatt cagaaacccc attaacgttc acgataacgc
     3421 gacgaaaacg ccgccagcgt tcaacaccga tgacgttagc accataacgt tcacgtaatt
     3481 tgagatcgtc cagtcgttga ccaatcattg gcgatccggg gcgaatagcc agacgtcgcg
     3541 cacgcccggt cagtcgatat tcacggataa gatcgcgaaa ggttcggcgc gtccagcctt
     3601 cgcgttgtgg ggtctgggta tcccctttca gcatgaaacg catcactaac atatacaaaa
     3661 tacccagcac cagcacgacc aggccaatag gtgttacgct aaagaaactg aagccgtgat
     3721 agccttcacg cagtaattca ctgttgacta ccaggttcgg cggcgtcgcc accagcgtca
     3781 tcatgccgct tatcagcccg gcaaaactta acggcatcat cagacgcgac ggcgacgttt
     3841 gcatacgcat ggcaacgctt aacaccacgg ggataaagat agcgacaacg ccggttgaac
     3901 tcataaacgc gccaagtccc gcgacggtca gcatcaacaa aaccaacatt ttgatttcac
     3961 tgttgcccgc cactttgacc agccatgtcc ccattacagt ggcaacaccg gtacggacca
     4021 aaccatcgcc aataatgaac aaggcggcaa tcaggacaac gttaggatcg gaaaagccgg
     4081 aaaatacttc ggggaccgtc agcgttccgc ttaatgcaaa cgcgacaata acaaacaaag
     4141 cgaccgcatc catacgcact ctgcccgtcg caaacaagac gatggcaacg gccagtaatg
     4201 aaagaaccca aatcaattca ccgttcacaa cttatccttg ttaattgagg gggataactt
     4261 gattctgcca taaaaaagcc ccgacgagac ggggctaaat catgatcagg tgtttcactg
     4321 aataataaca tcgccatttg gctgtttggt cacagtaatt tgctccagac tgtggaggac
     4381 taaatcgact tcattcaggc gcggggtatc tgccggggca ttaaccgcaa taacatggca
     4441 acccgccgcc agacctgaaa gcacgccagc gggagcatct tccaccacaa cacactcctg
     4501 cggcgcaagc cccagcagct gcgcgcctaa cagatacgca tcaggttctg gttttccgcg
     4561 cttcacccgc tcagcggtta caaacacctc tggcgcggga agccccgcta ttttatgacg
     4621 cgctcgcgct accggcatgg agccagaagt cacaatagcc cacggaatac ctgctttatt
     4681 caaatgattg agtaaggcga ttgcccccgg aagcgcggta ataccttcgg tttccgtggc
     4741 ctcgatgtgt tccagacgcg taaactcggc ggcaatgtct gcctcggatt tgcccgccat
     4801 aaaatggcgc agagaggtga tcgcctgttt accgtgaatg aaagccagca cctcttccgg
     4861 cgataaccca tgacgtctgg cccagttgct ccacgcccgt tctaccgcag gcagggaatc
     4921 caccagcgtt ccatcaagat caaacagaaa acctttgcac cgcacgcggg cctcctcagg
     4981 cgttaataat ttgattaatt tcgttggcgc tcaaatggta ctgacgcggg caggcatgcc
     5041 acacattaag catgcgctga tatttttccc acattggcgt ctgggcgtta aaaccgtgag
     5101 ttccagcgtc aaaatgggta tagcgccctt ccacattaac cataaagcgg acataaccga
     5161 ggtaacgtgc ttcagtggcg gcgtcaaagc cgaggaaggt gacacgacgt tcatcgatgg
     5221 attgctggtc ctgcaaatta gaccaggaaa catgcaacgc atgatacatc tccataatgt
     5281 cgatgatagt gcggcaggtt tcttctttca gctcgccaaa ctcgcgatcc agttcacgca
     5341 tctgtaatcc ataaccacgc tcaatgattg tttgcaggcg acggtaacgt tcagcatttg
     5401 ccggatcgag catagtcatc atcttgtact ggttagacaa aataagacgt tgcgcgttgg
     5461 tcatttccat tattgactcc tgtatcactc tactacggtg aaaaaaaaga aggctgagta
     5521 tgccttcttt tatatgcgta atcaggggtc aattacaaat catcaaggaa agttttatcc
     5581 agttgtttga aggcacgctt aagcgtgtca gctaatgcct ggtaatcagg cttgccttca
     5641 acgggtgcca acacctgtcc agactcctgc aatttaccgc gaacttcata aaaccagttg
     5701 aggattgcag gaggtaatgg cgttacagaa cgcttgccca gccaccacaa tccctgcatg
     5761 ggtaaactta aggcgaacag cgcagtggca actgccggcc caagctgacc gcccagggca
     5821 atctgccagc agagagtaaa tacggcgatc ggcggcataa aacggatcgc ataacgcgtc
     5881 atcttgataa cgcgattttc gacaaagact ggggcaaggc gtttttccag cggccacgtc
     5941 tttgagtaat gctgtccccg gcgaaacaag ctaaaaaaat taacagaacg attatccggc
     6001 gttgacatgc ttcacctcaa cttcacatat aaagattcaa aaatttgtgc aaattcacaa
     6061 ctcagcggga caacgttcaa aacattttgt cttccatacc cactatcagg tatcctttag
     6121 cagcctgaag gcctaagtag tacatattca ttgagtcgtc aaattcatat acattatgcc
     6181 attggctgaa aattacgcaa aatggcatag actcaagata tttcttccat catgcaaaaa
     6241 aaatttgcag tgcatgatgt taatcataaa tgtcggtgtc atcatgcgct acgctctatg
     6301 gctccctgac gtttttttag ccacgtatca attataggta cttccatgtc gagtaagtta
     6361 gtactggttc tgaactgcgg tagttcttca ctgaaatttg ccatcatcga tgcagtaaat
     6421 ggtgaagagt acctttctgg tttagccgaa tgtttccacc tgcccgaagc acgtatcaaa
     6481 tggaaaatgg acggcaataa acaggaagcg gctttaggtg caggcgccgc tcacagcgaa
     6541 gcgctcaact ttatcgttaa tactattctg gcacaaaaac cagaactgtc tgcgcagctg
     6601 actgctatcg gtcaccgtat cgtacacggc ggcgaaaagt ataccagctc cgtagtgatt
     6661 gatgagtctg ttattcaggg tatcaaagat gcagcttctt ttgcaccgct gcacaacccg
     6721 gctcacctga tcggtatcga agaagctctg aaatctttcc cacagctgaa agacaaaaac
     6781 gttgctgtat ttgacaccgc attccaccag actatgccgg aagagtctta tctctacgcc
     6841 ctgccgtaca acctgtacaa agagcacggc atccgtcgtt acggcgcgca cggcaccagc
     6901 cacttctatg taacccagga agcggcaaaa atgctgaaca aaccggtaga agaactgaac
     6961 atcatcacct gccacctggg caacggtggt tccgtttctg ctatccgcaa cggtaaatgc
     7021 gttgacacct ctatgggcct gaccccgctg gaaggtctgg tcatgggtac ccgttctggt
     7081 gatatcgatc cggcgatcat cttccacctg cacgacaccc tgggcatgag cgttgacgca
     7141 atcaacaaac tgctgaccaa agagtctggc ctgctgggtc tgaccgaagt gaccagcgac
     7201 tgccgctatg ttgaagacaa ctacgcgacg aaagaagacg cgaagcgcgc aatggacgtt
     7261 tactgccacc gcctggccaa atacatcggt gcctacactg cgctgatgga tggtcgtctg
     7321 gacgctgttg tattcaccgg tggtatcggt gaaaatgccg cgatggttcg tgaactgtct
     7381 ctgggcaaac tgggcgtgct gggctttgaa gttgatcatg aacgcaacct ggctgcacgt
     7441 ttcggcaaat ctggtttcat caacaaagaa ggtacccgtc ctgcggtggt tatcccaacc
     7501 aacgaagaac tggttatcgc gcaagacgcg agccgcctga ctgcctgatt tcacaccgcc
     7561 agctcagctg gcggtgctgt tttgtaaccc gccaaatcgg cggtaacgaa agaggataaa
     7621 ccgtgtcccg tattattatg ctgatcccta ccggaaccag cgtcggtctg accagcgtca
     7681 gccttggcgt gatccgtgca atggaacgca aaggcgttcg tctgagcgtt ttcaaaccta
     7741 tcgctcagcc gcgtaccggt ggcgatgcgc ccgatcagac tacgactatc gtgcgtgcga
     7801 actcttccac cacgacggcc gctgaaccgc tgaaaatgag ctacgttgaa ggtctgcttt
     7861 ccagcaatca gaaagatgtg ctgatggaag agatcatcgc gaactaccac gctaacacca
     7921 aagacgctga agtcgttctg gtggaaggtc tggtcccgac acgtaagcac cagtttgccc
     7981 agtctctgaa ctacgaaatc gccaaaacgc tgaacgcaga aatcgtcttc gttatgtctc
     8041 agggcactga tactccggaa cagttgaaag agcgtatcga actgactcgc aacagcttcg
     8101 gcggtgcaaa aaacaccaat attaccggcg ttatcgttaa caaactgaac gctccggttg
     8161 atgagcaggg tcgtacccgt ccggatctgt ccgagatttt tgacgactcc accaaagcaa
     8221 aagtgaacaa cgttgatccg gcgaagctgc aagaatccag cccgctgccg gttctcggcg
     8281 ctgtgccgtg gagctttgac ctgatcgcga ctcgtgcgat cgatatggct cgccacctga
     8341 atgcgaccat catcaacgaa ggcgacatca atactcgccg cgttaaatcc gtcactttct
     8401 gcgcacgcag cattccgcac atgctggagc acttccgtgc cggttctctg ctggtgactt
     8461 ccgcagaccg ccctgacgtg ctggttgccg cttgcctggc tgccatgaac ggcgtagaaa
     8521 tcggtgccct gctgctgact ggcggctacg aaatggacgc gcgcatttct aaactgtgcg
     8581 aacgtgcttt cgctaccggc ctgccggtat ttatggtgaa caccaacacc tggcagactt
     8641 ctctgagcct gcagagcttc aacctggaag ttccggttga cgatcacgag cgtatcgaga
     8701 aagttcagga atacgttgct aactacatca acgctgactg gatcgattct ctgactgcca
     8761 cttctgagcg cagccgtcgt ctgtctccgc cagcgttccg ttatcagctg actgaacttg
     8821 cgcgcaaagc gggcaaacgt atcgttctgc cggaaggtga cgaaccgcgt accgttaaag
     8881 cagccgctat ctgtgctgaa cgtggtatcg caacttgcgt actgctgggt aatccggcag
     8941 agatcaaccg tgttgcagcc tctcagggtg tagaactggg tgcaggcatt gaaatcgttg
     9001 atccagaagt ggttcgcgaa aactatgttg gtcgtctggt cgaactgcgt aagaacaaag
     9061 gcatgaccga aaccgttgcc cgcgaacagc tggaagacaa cgtggttctc ggtacgctga
     9121 tgctggaaca agatgaagtt gatggtctgg tttccggtgc tgttcacacc accgcaaaca
     9181 ccatccgtcc gccgctgcag ctgatcaaaa ctgcaccggg cagctccctg gtatcttccg
     9241 tgttcttcat gctgttgccg gaacaggttt acgtttacgg tgactgtgcg atcaacccgg
     9301 atccgaccgc agaacagctg gcagaaatcg cgattcagtc cgctgattcc gctgcggcct
     9361 tcggtatcga accgcgcgtt gctatgctct cctactccac cggtacttct ggtgctggta
     9421 gcgacgtaga aaaagttcgc gaagcaactc gtctggcgca ggaaaaacgt cctgatctga
     9481 tgatcgacgg tccgctgcag tacgacgctg cggtaatggc tgacgttgcg aaatccaaag
     9541 caccgaactc tccggttgca ggtcgcgcta ccgtgttcat cttcccggat ctgaacaccg
     9601 gtaacaccac ctacaaagcg gtacagcgtt ctgctgacct gatctctatc ggaccgatgc
     9661 tgcagggcat gcgcaagccg gttaacgacc tgtcccgtgg cgcactggtt gatgatatcg
     9721 tctacaccat cgcgctgact gcaattcagt ctgcacagca gcagtaatct cgtcatcatc
     9781 cgcagctttg cgctgcggat atctgaaccg gaaatagtca ctatttccgg ttttttattc
     9841 tcttaatttg cattaatcct ttctgattat cttgcttaac tgcgctgcat caatgaattg
     9901 cgccatctca ctttgcatac ttaccacttt gttttgtgca agggaatatt tgcgctatgt
     9961 ccgcaatcac tgaatctaaa ccaacaagaa gatgggcaat gcccgatacg ttggtgatta
    10021 tcttttttgt tgccatttta accagccttg ccacctgggt agttccggtg ggcatgtttg
    10081 acagtcagga agtgcagtat caggttgatg gtcaaacaaa aacacgcaaa gtcgtagatc
    10141 cacactcatt tcgcattctg actaacgaag caggcgaacc tgagtatcac cgcgtacagc
    10201 tgttcacgac gggcgatgaa cgcccgggtc tgatgaactt cccgtttgaa gggttaacct
    10261 caggatcgaa atacgggaca gccgttggca tcatcatgtt tatgctggtg attggcggcg
    10321 cgtttggcat tgtgatgcgt acaggaacca ttgataacgg tatcctggcg cttattcgcc
    10381 atacccgcgg aaatgaaatt ctctttattc ctgcgctgtt tattctgttt tcacttggcg
    10441 gcgcaatatt tggtatggga gaagaggccg tcgcctttgc cattatcatc gcaccgctaa
    10501 tggtccggct gggctatgac agtattacca ccgtccttgt gacctatatt gccacgcaaa
    10561 tcggttttgc cagttcgtgg atgaacccgt tttgtgtggt tgttgctcag ggtattgccg
    10621 gcgttccggt gctttctggc tccgggttgc gcatcgtggt gtgggttatc gccactctga
    10681 ttggcctgat ctttaccatg gtgtacgcct cacgagtgaa aaagaatcct cttctgtcac
    10741 gcgtgcatga gtccgaccgc ttctttcgtg aaaagcaggc tgatgttgaa caacgtccgt
    10801 ttacctttgg tgactggctg gtattgattg tcctgaccgc cgtaatggtc tgggttattt
    10861 ggggcgtgat cgttaatgcc tggtttattc ctgaaattgc cagccagttc ttcaccatgg
    10921 gtctggtgat tggcatcatc ggcgttgttt tccgccttaa cggcatgacg gttaatacca
    10981 tggcttcatc ctttaccgaa ggggcgcgaa tgatgatcgc ccctgccctg ctggtgggtt
    11041 tcgccaaagg gattttgctg ctggtcggta atggtgaagc gggtgatgcc agcgtgttaa
    11101 ataccatcct caacagcatt gccaatgcca ttagcggtct ggataacgca gtcgcggcct
    11161 ggtttatgtt gctcttccag gcggtattta atttcttcgt gacgtccggt tctggtcagg
    11221 cggcgttaac catgccgtta ctggcaccgc ttggcgatct ggtcggtgtt aaccgtcagg
    11281 ttaccgtgct ggcgttccag tttggtgatg gcttcagcca catcatttac ccaacctcag
    11341 cttcgttaat ggcgacgctc ggtgtttgcc gggtggactt ccgtaactgg ctgaaggtgg
    11401 gtgcgaccct gcttggactg ctgtttatta tgtccagcgt cgtggtgatc ggcgctcagt
    11461 tgatgggcta ccactaaaaa tgttaagagc cgcgattgcg gctctttttt cattctgccg
    11521 tttcagtctc gacggcttca tttttggcat tgcgcttcat ccacaacgcc agcgctttca
    11581 gcgagtctgg agtgaactca tcgcagcgtg cggtgatttc ttccggcgtc agccagcaaa
    11641 cttcactgac ttcatcttcc tgtagagcga agggaccgtg agagacgcag ctgaacaacg
    11701 cgccccagac acggcaattt ttatcttcga aatagaactg cccgtgctcg gcaaagggga
    11761 caccggcaat gcccaactct tcttccgctt cgcgacgcgc ggattccagc agctgctcat
    11821 cggcctggac tacaccgcct gcggtcgcat ctaacatgcc gggtaaaaag tcttttgtct
    11881 cggtacgacg ctggaccaga attttgccca tgccatcatg cacgacgata taagttgcac
    11941 gatgacgcag acactgtgcc cgcatttgtt cccggctggc ttgtgcaatg acttcgttct
    12001 cttcattgac aatatccacc cattcagtac ttgccaaacg acgctgttcc attatcagga
    12061 aaccttcttt ttctggcgct cttacggcgc attttgagtt gtgggtaaat tacggattaa
    12121 tcgcgacctg cgcaatgata ctttgatcat tgagtgcgat tacactaagt acgtcattat
    12181 ccagcatgcc ataactcgcc ggattaccgc ctttcggaat actcaccgag ccggggttga
    12241 agtggaaaat ctccccccgc tgttctgcca ctggcagatg ggtatgaccg tacaccagca
    12301 catcgttctg gtttaaagca ggtagatttt ccgggccaaa aagatgacca tgcgtcaaaa
    12361 acagacgttg tttttccagt aatacctgtt gccacggcgc ggttatcggg aaatgcagca
    12421 gcatttgatc cacttcgctg tcgcagttgc cgcgcacagc gataacctta tgtgccactt
    12481 cattaagccg ttcagcgact ttggctggcg cgtaaccctc cggtaaagca ttacgcgggc
    12541 catgattcaa cacgtcgcca aggatcacca gccactgggc accgctttgg gcaaacaact
    12601 ccagaacacg ttccgtcgcc ggtaacgacc catgaatgtc cgatgcaaac atcagtttca
    12661 tcactcactc ctcgtcgaaa aaacgtaagc tatgatactg gattctgtcg ttgctatcag
    12721 ccagattgct tcgccgaaag tgcaataaaa cgctggacag acgctcgctg ccactggaat
    12781 gtcgcataat ccaccaggcg ttccggcacc tcatcgccat gtagtaccag gcggttaatc
    12841 attagcgcca gatcagtatc agcaatgcac cattcaccaa ataaattcgg ctgacccagt
    12901 gctaacaaat gctctgccat cgcgaacagt ttctctgcac tggcttttcc ctcggccgtt
    12961 agtggcgctt ttttcgcccc cgcaaagaca acatccgtcg ggcgttcttc gcggatgggc
    13021 atcagatcgc tgcgcagcca ggcctgaatc tgtcgtgcac gcgcacgatt ttctaaatca
    13081 agcggataaa tacgttccca ggtcggtggc gcaaatcgat cttccagata ctccgcaatg
    13141 gcagaagatt cactcaactc aaaatcatcg atttgtaata acggcacacg gcgtgtctga
    13201 ccgtaacctt gccacgtcgg ctgcaaatgt tcaccgctgt cgaggtcgat ggtcttgata
    13261 tgaaacgaca ggcctttctc ctgcaacgcc acccaggcgg ataacacata aggggagaaa
    13321 aaatgggcat ctgaccaaag cgtaatagcg ggtttactca ttatgtcctc ggcaataaag
    13381 ggggcttgct atcaaactat agagcctttt tgtcactgtc acgtgacgaa tactcacgca
    13441 agtcgccgcg ctttctatac ttgtggtgtt cagatgatta gacaaccgga gctgcaatga
    13501 tcgatctcta tttcgccccg acacccaatg gtcacaaaat tacgctgttt ctcgaagaag
    13561 cagggctgga ttatcgcttg ataaaggtag acctggggaa aggaggtcag tttcgcccgg
    13621 aatttttgcg catttcgcct aacaacaaaa ttccggcaat tgttgatcat tctccagccg
    13681 atggcggcga accgctaagc ctctttgagt ctggtgccat tttgttgtat ctggctgaga
    13741 aaacgggact ctttttgaat catgaaacgc gtgaacgcgc cgtcacatta cagtggttat
    13801 tctggcaggt aggcggactg gggccgatgc ttgggcaaaa tcatcatttt aatcacgcag
    13861 ccccccaaac cattccttac gctattgaac gttatcaggt tgaaactcag cgtctgtacc
    13921 atgtactgaa caagcggctg gaaaattcgc cctggctggg aggcgagaac tacagcattg
    13981 cggatattgc ctgctggccg tgggttaatg cctggactcg ccagcgaatt gacctcgcaa
    14041 tgtatccggc agtcaagaac tggcatgagc ggatccgttc gcgccctgcc accgggcagg
    14101 cactgctaaa agcacaacac ggtgatgagc gttcggatag ttaacagaaa caggttctcg
    14161 tgtattattt cattctaagt aaaacaacgg agaacctgca atggcacaac ctgccgctat
    14221 tattcgtata aagaaccttc gtttacgtac gtttatcgga attaaggaag aagaaattaa
    14281 caaccgtcag gatattgtta tcaatgtgac gatccactac cccgccgata aagcgcgcac
    14341 tagcgaagat atcaacgatg cgctgaatta tcgcaccgta acgaaaaaca ttattcagca
    14401 tgtagagaat aaccgtttct ctttgctgga aaaattaact caggatgtgc tcgatatcgc
    14461 acgcgaacat cactgggtga cgtatgctga agtggagatc gataaactgc acgcgctgcg
    14521 ctacaccgat tcggtatcca tgaccttaag ctggcagcgt taaccaccat atcgggaggc
    14581 tgcatgaaca tagtgatcac cggagggacg ggattaattg gtcgccattt gattccacgt
    14641 ttgctggacc tgggccatca aatcacggta gtgacgcgta acccgcagaa agccagttcc
    14701 gttctcggcc cccgagtgac actatggcaa gggcttgccg aacaaagcaa cctcaacggc
    14761 gttgatgcgg taatcaacct ggccggagaa ccgattgctg ataaacgctg gactcacgag
    14821 cgaaaagagc gtctctgcca aagccgctgg aataccacgc aaaaactggt cgatttgatt
    14881 aatgccagcg acacgccacc gtcggtactc atttccggct cggcaacggg ctattatggc
    14941 gacttaggtg aagtggtggt taccgaagag gaaccgccgc ataacgaatt tacccataaa
    15001 ctctgcgccc gctgggaaga gattgcctgt cgggcgcaaa gtgacaaaac gcgagtgtgc
    15061 ctgctgcgta ccggcgtagt gctggcacca gacggtggta tcctcggtaa aatgctgccg
    15121 ccatttcgtt ttggcctggg cgggccgatt ggctccggtc ggcagtatct ggcctggatt
    15181 catattgatg atatggtcaa cggcattctc tggctgctgg ataacgagct gcgcgggcca
    15241 tttaatatgg tttcgcccta cccggtacgc aatgaacaat ttgcccatgc gctcggtcat
    15301 gcgctgcatc gcccgaccat tttgcgcgtc cccgcgaccg ccattcgact gttaatgggc
    15361 gaatcttcag tactggtatt aggtggacaa cgcgcgctgc caaaaaagct ggaagaagcg
    15421 ggttttgcgt ttcgctggta cgatttagaa gaggcgctgg cggatgtcgt tcgctgatgt
    15481 ggtttactgc aaacatccgc cagcggaaac gctgatgaat aaatatagat aattcagaac
    15541 tatttttagc ggactgcaac ttttacattt cagcggaagc ggactcgcaa attccccatg
    15601 aaatactccg gcagcgttaa ccaccggaga ggaaattatt tcagcgaacc tttaaggaac
    15661 tgttgcagac gagggctttg cgggttaccg aataactgct ccggcgcccc ttcttcttct
    15721 attttcccct gatggaggaa aatgacgtga gtagaaacat gacgagcaaa gcccatttcg
    15781 tgagtcacta ccaccatggt tttcccctct tctgccagtt gctgcataat acgcaacact
    15841 tcgcctacca gttcaggatc gagcgctgaa gtaggttcat caaacagtaa aacttccggt
    15901 tccatcgcta gcgcccgcgc gatagaaaca cgctgttgct gaccaccgga caggtgcacc
    15961 gggtatttcc cctgcgcacg ttcgtctatc ccgacttttg ccagatactt caccgcccgc
    16021 tcgcgcgctt cctgcttgct caggcccaac acctgaatcg gcgcttccat gacgttttcc
    16081 agcaccgtca tatggctcca gagattgaag tgctgaaata ccatcgtcag gcgtgtgcgc
    16141 aataagcgca gctgattttt atcggcgact ttgagttgac catctttatc gcgcaccaga
    16201 ttgatcgtct ggccattgac cacgatcgat ccatcgctcg gtttttcgag gaagttaatg
    16261 cagcgcagaa aggtactttt ccccgatccc gacgatccga tgatgcttat gacatctccg
    16321 gcattcgctt gcaatgagac ccctttcagc acttcgtgtt cgccgtagcg tttgtgcaaa
    16381 tcgataacgt ttaatttatt ctcggacatc gtattctcag tgcgttgaag aaggtttcac
    16441 atgctgcaac cagcgttttt cagctctacg aaagaggctg atcagaacat aagagatgat
    16501 taaatagagc accgcggcaa tgccgaaagc ggtaaaaggt tgatatgtgg cggcgttaat
    16561 atcgcgggca attttcagca gatccggcac cgtggcagta aatgccagcg cagtagagtg
    16621 cagcatcagg atcacttcgt tgctgtatgc cggtaacgca atacgcagcg cagaaggcaa
    16681 aataatgcag cgatacattt taaaggtcga gaagccatag gctctggcgg cttcaatttc
    16741 cccatgcggc actgaacgga ttgccccggc aaaaatctcg gtagtgtaag cgcaggtgtt
    16801 aagcgtcagc gccagcacgg tacagttcag gccactgcga aaaaaggcgt taaggaactc
    16861 ggttccctta acaatctcca gcgtgtacat gccggaatag aacaccagca actgaacata
    16921 cagcggcgta ccgcgaaaaa tataggtaaa taaccagatt ggaaactgga tgtatttatt
    16981 acttgagacg cgaccaatcg ccagaaacag cgccagaact ccgcctatca ctaccgacaa
    17041 aatcagcagc cacagggtga tcgccacgcc agtaaagcga taaccgtcag tccacagcag
    17101 tggtttccag tattcatgta agatttcgat cacaggtcag ccctcttcac acccacggag
    17161 tagcggcgct caaggaacag cagcacacca ttggaaacgg tggtgaaaac aagataaatc
    17221 acgccgcaga cgatagcgaa atagaacggt tcccaggtac ttttgcctgc cagctgcgtg
    17281 gctttgacca catcttccag gccgagtaac gaaaccagtg cggtagattt gaggatcacc
    17341 tgccagttgt tgccaatgcc tggcagggcg taacgcatca tcgccggaaa catgatccgc
    17401 cgaaacactt gcccacgagt aaaaccgaac gccgtcgccg cctctatatg tcctttcggc
    17461 actgccataa acgcgccacg aaacgtttcg gtaaaataag caccgtagat aaaaccgaga
    17521 gtgataatac ctgcgaccat cggatcaata tcaatctgcc cgacgcccat cgcctccgtc
    17581 accgtgttta gcgcaatctg caaaccgtag aaaatcagca gcatcaatac taaatccggc
    17641 acgcctcgaa tcagcgtggt gtacccttcg aaaataaggc ccgaaagccg attttgtgag
    17701 agcttaccgc cagcgccaat taaaccgatg attacagcga gcactacaga gctgatagcc
    17761 agctccagcg tgacgagcgc accctgtaaa ataacacttg aaaacccata caacatgctg
    17821 cctgtcctgt cgtgtgtggt gaattaccgc ctcttgtctc cctccggtta cccgaaggga
    17881 gcgggctttc tttcaccgat ggcgattaac caccataaac atcaaaatcg aagtactttt
    17941 tcgctaattt ctcgtaagta ccgtcagcgc gcatttcggc aaaggctttg ttcagtgcct
    18001 cgcgcagttc gttatcttct ttacgcaggc ccatgccggt ccctacgcca aacagttttt
    18061 catctttaac agacgggcca ccgaatttgt aatctttacc gacgggttgt ttgaggaaac
    18121 cttcgctggc tgcgacctca tcctggaacg cggcatcaat acgtccggca gtcaggtcag
    18181 aataaatgtt gtcctgcccc tgataagaaa cgatttcaat gccttttggt gcccagtgtt
    18241 cgttaccgaa cgtctcctgg gtggtgccct gcaatacgcc tacccgtttg cctttcagcg
    18301 attcgactgt cggctgaatg tcggaatttt tcgccaccac cagacgagaa tcggctgcgt
    18361 acagtttgtc ggtgaaggct atttcttgct gacgtttttc cgtaatggaa agcgatgaca
    18421 tgatggcgtc aatcttcttc gcttttaagg acgggattaa cgcatccagt gggttttcga
    18481 caaacgtaca ttgcgtattg atgcgtttgc acaactcctt ggccagatcg atatcgaagc
    18541 caaccagttc accttgtgaa ttttttgatt caaatggcgc ataggtcgga tcggtaccga
    18601 tgcggatgtt ttgcggaatc gcagcaaatg ctgcagttgc gctggagaag gccagaacca
    18661 gagagagcga tagcaccagt tttttcataa tgtcctcaac tgacagtctt tttatcggga
    18721 atatttacag gtttgttaca cttatcgtgc cataaaattg accatcaagg caaaatatcc
    18781 cgccccgcta gacgtattat ttttcattaa aaatgcttaa gtatgcattt caatgcacca
    18841 cgatagtgca acaaacctgg cgtgcaccat gatgatcatt ccatcaggta cagcttccct
    18901 gcgacgtatc agtcaccgta gacattaaag tcaaaatact ttttcgccat cttgtcgtag
    18961 gtgccgtcct gacgcagctc gccaagcgcc ttattaaacg cagccgtcag ttccgcatca
    19021 tctttacgta gccctacacc ggtgccgtca ccgaagtatt ttttgtcttt cactgatggg
    19081 cctgcaaagg cgaaatcttt accggcgggt tgcttaagga aaccttcgct ggcagcaact
    19141 tcatcttgta acgcagcatc cagacgtcct gcagccagat cggaatagac taaatcctgg
    19201 ttggcatagg ccaccacatc cacgcctttg ctacgccagg tctcgttagc gtaagcttcc
    19261 tgggttgatc cctgcagcac accaacatgt ttacctttca gtgaatccag cgttggctga
    19321 atcggtgaac ctttggcggc aatcaaacga gaatctgcgg cgtacagctt gtcggagaaa
    19381 gcaatctcct gctgacgttt atcggtaatg gagagcgacg aaataatggc gtcgattttt
    19441 ttcgctttca gtgaggggat cagcgcgtca aagtcgctgg caacccaggt acatttcacc
    19501 tgcatccgtt tgcacatctc gttaccgaga tcgatatcaa agccaacaaa gtcacctttg
    19561 gcatctttcg atgagaacgg tgcgtaggtg gtatcggttc cgatacgtac cgtctccggt
    19621 agcgccgcat agctggaagc cgctgcggag agaccgacta acaaagacag agcgagaatc
    19681 gacttcttca tacataaccc tcaagtgaaa atggcttttt tatgttgtgt attgtgttgt
    19741 gtgtttgcag gctctttcat gcaggtctta tgccatcttg acgacagcag taacattcaa
    19801 cgttaaatat gttaataaga agttgcatta ttgtcctaaa gttgaagata gcaggtatag
    19861 cggttgaatc gcagcgtttc gattgaatgg cagaaacaaa atgtcgagga tttgatcgcg
    19921 gttgcaaaat tgccctgaaa cagggcaaca gcggagttat gcgccctgcc agcgggcaaa
    19981 gagatcttca ggaagggtta ccgcaaactg gtcaagaaca cgattaaccg tctgatttat
    20041 cacatcatca agggattgtg ggcgatgata aaacgccgga acgggaggca taatcaccgc
    20101 accgatttct gccgcctgag tcattaaacg cagatggcct aagtgcagtg gcgtttcacg
    20161 cacgcagagc accaacgggc gacgctcttt cagcaccaca tctgccgcac gggtcagtaa
    20221 gccatcagta tagctatgga caatgccgga cagggttttg attgaacagg gtaaaatcac
    20281 catccccaac gtctggaaag aaccggaaga aatgctggca gcaatatcgc gcgcatcgtg
    20341 tgtcacatca gctaacgcct gcacttcgcg cagggaaaaa tccgtttcga gggataaggt
    20401 ctggcgcgcc gcctggctca ttaccagatg cgtttcgata tctgtgacat cgcgcagaac
    20461 ctgtaataaa cgcacgccat aaatcgcgcc gctggcaccg ctgatgccta caatgagtcg
    20521 tttcataaat agttgccctg tcagacttgc aggcagactt tgcaggattt cggcgggagt
    20581 tgcaagtcag ggtgccagac cggcaccctg agcgaaggca tcatccttcg ttatgcattt
    20641 cgagattttc cacttcgttc tgacgttgca ctgctttggc gtcgtcatta cgtaaagtat
    20701 cgaggaaatc gaggtagccc tgatcaacgt ctttggtgac gtagacgccg ttgaacaccg
    20761 agcattcaaa ctgctggata tccgggtttt cagcgcgaac ggcttcgatc agatcgttca
    20821 gatcctggaa aatcaacccg tcagcaccga tgatctggcg aatttcatct acttcgcgac
    20881 cgtgagcgat cagttccgtg gcgctcggca tatcgatacc gtaaacgttc gggaagcgaa
    20941 tttccggtgc cgcagaagcg aggtacactt tcttcgctcc ggcttcgcgt gccatctcga
    21001 taatctgctc agaagtggtg ccacggacga tagagtcgtc gaccagcagg acgtttttat
    21061 cgcggaactc ggcgcggttg gcgttcagtt tacggcgcac ggacttacga cgcagctgct
    21121 gacccggcat gatgaaggtg cggccaacat agcggttttt aacgaagccc tggcggtacg
    21181 gtttgcccag aatacgcgca atttccagcg cgatatcgca ggaagtttcc gggatcggga
    21241 tcaccacgtc gatatccaga tcttcccatt cgcgggcaat tttctcgccc agcttcgtgc
    21301 ccatattcac acgcgcgctg taaacggaaa ttttgtcgat gaacgagtcc gggcgagcaa
    21361 agtatacata ctcaaacagg cacggattgc tgaccggatt gtcagcacac tgacgggtaa
    21421 acaactgccc ttcttcagtg atgtaaatcg cttcgccagg ggcgacatca cgcaggaact
    21481 caaagcccag cgtatcgagc gcaacgcttt cggaagcgac catatattct gtgcgattat
    21541 cgtcaatatc acgtttgccc agtacaagcg gacgaatccc gttaggatcg cggaaagcaa
    21601 ccataccgtg gccgataatc atcaccacac aggcatacgc gccgcggatt aagcggtttg
    21661 tagcagcgat ggcggcgaaa atgttgtcgg cttccagcgg gtagtggcgg aagttgtcca
    21721 gctcgctggc gaagatatta agcagaattt ccgagtcgga agtggtgttg atgtggcggc
    21781 gtttttcttc aaacagtttt ttacgcaact cgtgagcgtt ggtcagattg ccgttgtggg
    21841 caagcgtaat gccatacgga gagttaacgt aaaacggctg cgcttcagag gcgctggagc
    21901 tgccagccgt agggtaacgc acatgaccaa tgcccatatt gccctgcaaa cgctgcatat
    21961 ggcgagcttc aaatacatcg ctcaccagcc cgttcgcttt ccgcaaacgg aagcagttat
    22021 tggcatctat ggtgatgatg ccggcggcat cctgaccgcg atgctgaagc accgttaagg
    22081 catcataaat cgactggtta accggcataa caccggcgat accgacaata ccgcacatac
    22141 gtctttttcc tcgttaagcc acatctcaga gcacttacgc tctgggcaag aaacttgacg
    22201 agctttgcag ataatcaaaa aaccatctga tgataaaact gaactgcggg atcagctgtg
    22261 atttgctcca gtcttcgctt ttcgacaccc cggtaaagga gtcgagaaag aagagaatgg
    22321 cagcaacaat caacacaccg cgcaaggcac cgaaacagac gcccaacacc cgatcggtgc
    22381 ctgacaaccc cgttttctcc accaactggc ctatcacgaa gttcacgata gcaccaacga
    22441 tcagggtagc gataaacagt accgcgatgg caatcccatt tcgaaccagt tcgtcttcaa
    22501 agcccgtaaa ccagactgac aggtaagtgt agtaatgact ggcaacaaag aaagcacaac
    22561 cccatgtcac cagcgataac gcttcacgaa caaagccgcg gatcaggcta accagagagg
    22621 aaaaagcaat caccgcgatt atggcgtaat caatccagac catatgtgtc ccacgatttt
    22681 acgccctgtc atcctgttcg gggcgcattc taacagaaaa agaaaacgtt tgcgtaggga
    22741 tttccttccc gcgcatcaat aaaaatggcg ctgaaaaaat attcaacgcc atcgactttt
    22801 tatgcctttg cggcatcggg caatgcgtgt cggatgtggc gtgaacgcct tatccgacct
    22861 acggctctac ccctgcacag gcctgataag acgcactggc gtcacatcag gcaacggctg
    22921 tcggatgcag cgtgaacgcc ttatccgacc tacggttcta cccctgcaca ggcctgataa
    22981 gacgcgctgg cgtcgcatca ggcaacggct gtcggatgcg gcgtgaacgc cttatccgac
    23041 ctacggttct acccctgcac aggcctgata agatgcgcca gcgtcgcatc aggcaaaacc
    23101 gtattaattc ggtgtatagc ccattaccac accacttaag ccagaaagct gcttgagctc
    23161 acccagcgat cctttcagct tctctttgga agcatctggc ccaaccagaa tacgggtaat
    23221 tttaccctgc actggcgtgg atggcgacgt ataaacccga taaccggcac cgcgcagctt
    23281 accgacaatc tcattcactt tatcggcatt tttcagcgcg cccagttgca caacataggc
    23341 tttacccgtt ggcgctgctt tttcttccac gaccggcttc ggttctggct tcggcgcagg
    23401 tggtgcttca accttcggtt taggcgactc caccggtttc ggcttcggtg ggacgactgg
    23461 tgcaggttcc ggttcaaact cggtgttatt ggctgcaata gtggccggat cgagcgacgg
    23521 tgctgcggca tcgcctgccc gcacctcttc cgctgcgcct tccggcggct gcgtcggcaa
    23581 cgcctgggtg gcggctggca tcatatcagg ctcatcgcga tcgcccgctt tcggcaccag
    23641 cgggatagcc gcgaactcat cctgataatg ttttttctgc ccgtccagca gccctggaag
    23701 tacaatcacc cccagcgcca ccagcacgat cgtgcccact aaccgattct gaaacttact
    23761 tgccaccgct tctcctcgcg tcaatcactt ccatgacatg tgcgaccgtg tggaacgaac
    23821 cacacaccag cacggtgtct tccgctttag cgtccgccat tgcggcatcc catgcctgcg
    23881 caacgctatc aaatgatttg ccgttaccca aatgctcaag cagttgttct gccgtggcac
    23941 cgcgcggccc ttccagtggc gcacaatacc agtcatcaac cacgcttttc aaccaggcca
    24001 gagttccggc aatatcttta tcatgtagca taccgataac cgccagcacg cgcccgtttt
    24061 tcggtagcgc tttcatacgc ccggtgagat attccgccgc atgtggatta tgcgcgacat
    24121 caaaaataac gcgtggcgac tcgctcacaa tctggaaacg tcccggcaaa attgcgctgg
    24181 caatcccgtc gcgaatggca ttttcactga cctccagccc gctggcacgc agtgccgcca
    24241 gcgccgttgc ggcattcggt tgcgggacaa gcggcaacgg cagattttcc agcgtgccgt
    24301 gagcatcgct aaacgtccag tcatgatcgg tgacggaata gttccactca acgccccgac
    24361 gttgtagcag tgcacctttt tcctgcccca catcagcaat ggtagaaggc atttccggct
    24421 caccgacaat tgccggtttt tcgctgcgaa agatacctgc tttctcgcga ccaatacttt
    24481 cgcgatctgg ccccagccag tcggtatgat ccagcgcaat actggttact accgcgacat
    24541 cggcatcgac aatattggtt gcgtccagac gaccgcccag ccctacttcc agaatcacta
    24601 cgtcaagttg tgcctgcttg aacagccaca atgccgacag cgtaccgtac tcgaaatagg
    24661 tcagggaaat atcaccgcgt gccgattcaa tctccgcaaa agaagcggtg tgggccgatt
    24721 ccggcaattc ctgcccctgc acacgtacgc gctcggtata acgcaccaga tgcggcgaac
    24781 tgtagacgcc cactttgtac cctgccgcca tcagaatcga ctccagcgta cggcaggtgg
    24841 tgcctttgcc attcgtaccc gcaacggtaa acacaaatgg cgctggtttc agaacgccaa
    24901 gacgcgccgc gacctggctc acgcgctcaa ggccgagatc gatagtttta ctgtgcaggt
    24961 tttccagata agaaagccac gaagccagag gcgacgcggc ttgaggagtg cgtttgataa
    25021 tcatggtatc cgctgattcg ttacggtgag aatagcaaaa gggcagagcc agtggccctg
    25081 cccttatcag ttatcaggcc tcaggttcct gatccggtac cggtggcacc actacgcctt
    25141 cacgcggcgc ttcaggattc ggcgctggca gattcatcaa cttcgccaga atgctcgcca
    25201 gtttcagacg catttccgga cgacggacga tcatgtcgat cgcgcctttc tcgatcagga
    25261 attcactgcg ctggaatcct ggcggcagtt tttcgcgaac agtctgttcg ataacacgcg
    25321 gaccggcaaa gccaattaac gctttcggtt cagcgatgtt gagatcgccc agcatggcga
    25381 aacttgcaga aacaccgccc atggtcgggt cggtcagcac ggagatgtac ggcaagccgc
    25441 gctcctgcat ttttgccagt gccgcagagg ttttcgccat ctgcatcagc gacatcagtg
    25501 cttcctgcat acgtgcgcca ccagaggcgg agaagcagat cagtgggcag ttatcttcca
    25561 gcgcctgctc aacggcacgc acgaaacgcg cacctaccac agaccccatg gaaccgccca
    25621 taaaggcgaa ctcgaatgcc gcagcgacaa ccggcattcc atacagagtg cctttcatca
    25681 ccaccagcgc atctttttcg ccggtttctt tctgcgcaga tgccagacgg tctttatact
    25741 tcttggagtc acgaaacttc agcacatctt tcggctcaag ctcgctaccc agctccacaa
    25801 ggcttccttc atctaacagg ctatgcaggc gattacgcgc tgtcatacgc atgtgatggt
    25861 cacacttcgg acagacctca agattacgtt ccagctcagc gcggtataaa acctgaccgc
    25921 agctatcaca cttagtccac accccttcag gaatgctcgc cttgcgggtg ggagtaatgt
    25981 tgcttttaat tcgttcaatc cagctcatta gggacctttc tgtctgaacc tggttcgatg
    26041 ccagttttat ctttggggac gcataatgcc atttttgccc ccaacagacc atgaatgttg
    26101 cacattaaaa cataacagcc cgaaactttg gataaaaaag tggtcgaacc gcagagttac
    26161 tttttatttt gcggcgcgtg ccgctgcgcg tttgtggcga ataaattcga aaacacctgg
    26221 caatattgaa acaaaaataa tgccaacaat catcagttta aggttgctct gaatgaaggg
    26281 gagcgtgccg aagaaatagc ctgcgtaggt aaaaagcagt acccacaaca gtgcgccaat
    26341 cacgttataa gcggcgaaat gacggtacga catgtggccc attcccgcaa caaacggggc
    26401 gaacgttctg acgatcggca caaaacgggc gagaataatc gttttgccgc catgtttctc
    26461 ataaaactga tgggttttgt cgagataact gcgacggaaa attttcgaat tgggattact
    26521 gaataacttt tcaccgaaca accgcccgat agtgtaattg accgcgtcac ccacaatcgc
    26581 ggcgatcagc atcagcacca ccatcatatg gacgttgaga tcgttggtct ccagtgatgc
    26641 caacgctccg gcaacaaaca gcaatgaatc acccggtaaa aacggcgtta ccaccagacc
    26701 ggtttcacag aacaaaatta agaacaaaat ggcataaacc cagacgccgt actccgcgac
    26761 cagctctgcc agatgcacgt caatgtgcag gataaaatcg atgaggaaat aaatcaggtc
    26821 catatttgcc tatgccttgt actcgtcatc tatcaggctg taactacgtt ggctacagcc
    26881 tgaaatgttc cgagcattat tctcgttagt ccgccagaaa tagcgggccc attggcggtt
    26941 ttggaagatc atatcggtca gggtaatcca ccgcgaccag atatagccct tccgcttttg
    27001 ccgttgctgc cgccagcgtt ctgtcctttg ccgccagcaa ctctgctatc cagctctccg
    27061 gctggttgtg ggcaccgact tccatcaggc tgccgacaat attcctgacc atatgatgta
    27121 caaaggcatt cgctttgata tctaccacca cataaggacc gtgacgcgtg acattaatat
    27181 gcataacgtt gcgccacggg gtccgggact ggcactgcac cgcacggaac gaggtgaaat
    27241 cattctcgcc cagcaaacat tgcgcagccc gatgcatccg ttcagcgtcc agcggttcgt
    27301 aaaaatgggt taccccttta ctcagtaccg ccgggcgcag ccgatgattg tagatgatgt
    27361 agcgataacg gcgagccgtg gcgctaaatc gggcatgaaa atcatcaggt acagctttaa
    27421 cccaacgcac ggcgatgtca ccaggtaaat tcgcatttac gcccaacgtc cacgcagcgt
    27481 ctttgcgctg cgcggtcgtt tcgaaatgca caacctgccc ggtaccgtgt acaccggcgt
    27541 cagtacgccc ggcgcagaag acggtgatgg gttcgttcgc cacctgggag agcgcctttt
    27601 ccagcttctc ctgcacactg cggacttcat tctgccgttg ccagccgtaa tacctactgc
    27661 cgtcgtactc aatgcccagc gcaattttat aaactggcgg ttgttgctgg tcggacatta
    27721 gtacagatac tcctgcacca gtttctcggc aatttttact gccatcagcg cgccgccaaa
    27781 gcgaacgtta tcggccaccg accagaactg aacttgctcc ggcataccgt agtcattacg
    27841 tacgcagcca acagaaagat gcggcgtacc agaagcatcc cctacctgag tcgggaattc
    27901 gttctcttca gagagcacaa tatcttcgcc ctgagcaaac gcatcacgcg cttcttctgc
    27961 cgccagcgga cgcagtgctt caaagttgac catctgggca tgaccgtaga ataccggtgc
    28021 ctggacgacg cttgccgaaa tcatcagccc ttcgtcctgc aggattttgc gtacttcgtc
    28081 aacgatacga cgttcttcac gcacgctacc ttcgctatcc ggcagtaacg gcagcatgtt
    28141 gaacgccagc tgacgcccga agaaatcttc ttcgtcaatc ggaatgccat tgagtaattt
    28201 agcactctgc cccgctaacg catcgaccgc ttttttgccc tgggcggagg ctgaaatcag
    28261 gctggtaacg ctgatacgtg ataaaccgcc ctgatcaatt agcggtttca gtgccgccag
    28321 cagctggctg gtcagactgt ctggtacggc gatgacattc cggttgcggt aatcggtcag
    28381 tacaaacggg tttacttccg gcaccaccag cggtacgtcg ggttcgagag caaacaatcc
    28441 gctgctgtcg atcaccagac aacctgagtt ggtcgcttct tcaacccagg cagcggtagc
    28501 ctctttgcct gcgacaaaaa atgccagctg cgcctgcgtc cagtcgaatt cagcagcatc
    28561 ctgcacggtg attgtcttac caccaaagcg cagttgttcg cctgcgcttt cgttacgtgc
    28621 cagtgcataa atttccccaa ccgggaactg acgttcagcc agcgtttcaa gcagggcttc
    28681 gcccacagcg ccagttgcgc ccaggacggc aatgttccag ccttcagaca tggtggttta
    28741 ctccagaaat agcaaagctc cctgccaata ttacagcaga gagcatgaag aagagattaa
    28801 cgtgccggat gatgaacggc gttaaaaccc agtttacaca gcaatgatgc cgcgctggcg
    28861 tcatcacaaa tcacatacag agatgaccat tcacggcgct caagatagtt tttgcgcagc
    28921 ttatcgaact cacccggtat tccggcgact ttacgcagcg gtgcgtcatc gcggcgcaca
    28981 tcatacacca aatgcaccag cctttttagc gtcggttgat cgagcggacc atgcagcgta
    29041 atgcggccaa actctggcgc gggcagcaac gtatccagcg caacgtgttg ttcatgccca
    29101 ataagcttgc tataggcttc aaacacttgc gtagtaccgc gtgctttacc ttccagggta
    29161 tagcctgcga tatgcggtgt gccgatatcc acttttttca gcaattccac gttgagttcc
    29221 ggttcgcctt cccagacatc cagcactacg cttaacttct ggccttcgtt cagacaggtc
    29281 aatagggcag tattatcgac gactgcgcca cggcaggcgt taatcagaat cgctcctggt
    29341 ttcaggctac ggatcagttt ttcatccgcc agatgcagcg ttttgtacgg cccgtctttg
    29401 aagagtggcg tatggaaagt cagaatatcc gcatgctgaa ctaattcatc cagcgagcgg
    29461 aaatccccct catccccgcg gtcggcgcga ggcggatcgc aaagcaaggt tgtaatccct
    29521 aacgcttcca gtcgcgcctg taaacggcga ccaacgttac caacgcccac gatccccact
    29581 gtacggtcgt gcagtgaaaa tccatcgcgt tcggcaagca tcagcagtga ggagaaaaca
    29641 tattccacca ccgcaatcgc attacagcca ggtgcagcgg aaaaaccaat tcccgcctgc
    29701 tttaaccatg cttcatcgac atggtcggtc cccgcggtgg cagtgccaac aaatttaatg
    29761 ggttttcctg ccagcaaaga ttcattcact ttcgtgaccg aacgcaccat aagcgcatcc
    29821 gcgtctgcca gttgagcgac aggaattgga cgcccgggaa ccgcggtcac ctcacccaaa
    29881 cggctaaata agtcgcgggc ataaggcata ttttcatcaa caaggatttt cacgtttgtg
    29941 ttacctgtat gagacgagag ttaaccagac aagtgtgcca taatctcgcg gccaggcata
    30001 cttgcgaaga tttcaggtat aaggatacgt aatgatacaa cctatttccg gccctcctcc
    30061 tgggcaacca ccaggccagg gagataacct gccgtctggc gcgggcaatc agcctttatc
    30121 cagtcagcaa cgtacttcgc tggaaagctt aatgacgaaa gtgacctcac tgacgcaaca
    30181 acaaagagca gaactgtggg cgggtatcag gcacgatatt ggtctgtcgg gagattcacc
    30241 gctgctttcg cgtcacttcc ctgccgctga gcacaatctg gcgcaacgtc tgctggccgc
    30301 gcaaaaaagc cattctgccc gccagctttt agcgcaatta ggggagtatt tacgtctggg
    30361 gaataatcgt caggcggtca cggattatat ccgtcataac tttggtcaga cgccgctgaa
    30421 tcagctctca ccggagcaat taaagaccat tctcaccctg ttgcaggaag ggaagatggt
    30481 tattccgcaa cctcagcagc gcgaggcgac cgaccgtccg ttattaccgg cggagcacaa
    30541 tgcgctcaaa cagctggtga ccaaacttgc ggcggcaacg ggagaaccca gcaagcagat
    30601 ctggcaatcg atgctggaac tttccggggt gaaagatggc gagttaattc cagcgaaact
    30661 gtttaaccat ctggtgacct ggctacaggc gcgtcagacg ctaagccagc aaaatacgcc
    30721 gacgctggaa tcactccaga tggcgctaaa acaaccttta gatgccagtg aactggcggc
    30781 gttatcggca tatatccagc aaaaatatgg cctttctgcg caatcatcgc tttcttctgc
    30841 ccaggccgag gatattctta atcagcttta tcaacggcgg gttaaaggga ttgagccgcg
    30901 tgatatgcaa ccgctgctta atccttttcc accgatgatg gacacgttgc aaaatatggc
    30961 aacgcgtccc gcgctgtgga tactgttagt cgcgattatc ctgatgctgg tctggctggt
    31021 tcgttagccc cgacgaaatg acagtatcgt gacaataatt cccagcaccg cagagatcgc
    31081 cccggcaaga aataccgaag agtaaccaaa cgtggtcgcc agcattcccg ccagcggccc
    31141 ggagacgccg agggcgatat cctgaaacgc ggcgtaaccg cccagtgcgg tgccgcgaac
    31201 ttgtgagggg acgcgtttaa ccacctccac gcccagcgca ggaaagataa gcgaacatcc
    31261 ggctccggtt aacgccgcgc ccgctaatgc gacccacgca ccaggggctt gccagagcag
    31321 caacaagccc accgtttcta caagcagaga gacgatcgcc actttcacgc cgccaaaacg
    31381 gtccggcatc cagccaaaca tgacgcgcat cacgacaaat gcgccgccaa acgcggtaag
    31441 agtaaagccc gccatcgccc atcctttact ggcaaagtag agcgaaacga aagtcccgat
    31501 aaccgcaaaa ccaacgcctt gtagtgccag acctaaccct ggtttccaga taagcccgac
    31561 aacgctccac agcgatggac gttctcccgc cagggccggt actttgcgca ctgtgccgtt
    31621 acaggcccac gccagtaagg gtaatgccat tgtggtgagc gccagtgcgg caaaaccgta
    31681 atggctatga atcaacaggc caagcggcgc accaacagcg agggcaccgt aaatcgccat
    31741 tccgttccat gacatcactt tgccggaatg ttttggccca acgatgccta atccccaggt
    31801 cagagcgccc gtcagtaact ggctttcacc aaagccaaga atcaaacgcc cgacgaccaa
    31861 cagggcaaat ttgaacggtg cggagacggg caaaatcgcc gccaacagca gcgcgccgcc
    31921 agccagacca caagctaaca tcccctgaag cgccgaacgt tttgcgccat attgatcggc
    31981 cagtcgcccg gcgtaaccac gcgtcagcac cgtagccaga aactgaatgc cgacggcaat
    32041 accgaccatg gtgttgccat agcccagttc atgatgaaca aacagcggga taaccggcaa
    32101 cggcagccct acggtcatgt aggtgagaaa aaccgcaaaa gcgatgcgga agagcgaaaa
    32161 attggcagaa gatcgtgttt cggtttggct tacagcagtc atgcattact ccagaatgca
    32221 gcgcaaggcg aggaatttcc ccgtctcatc tctctggttt cagggttacg gtgcgttggc
    32281 aggatttaac gcgtacgtct tttcagaagg aaatcgacaa agcgggaagt ttgcctggaa
    32341 caggcggcga ttgtcaataa tgtgaaaaag ggaaccatca ggttcccttt tgcgttagtg
    32401 tcggatgccg cattggcgcg taatgtcgga tgcgacgctg gcgcgtctta tccgacctac
    32461 tccgaattaa tctttcagct tgcgcattac cagcgtggcg ttggtgccgc cgaagccgaa
    32521 gctgttagac ataactgtgg tcagttcgcg atcggtcgtt tcggtcacga tgttcagacc
    32581 cgcagcctgc tcgtccagct cttcaatgtt gatgctcggg gcgataaaac cgtgttccag
    32641 catcagcaga gagtagatag cttcctgtac gccagcagcg cccagagagt gaccggtcat
    32701 tgctttggtt gcagaaatcg ccgggctctt atcgccgaac acttcacgga tagctgccag
    32761 ttctttcacg tcgccaaccg gagtcgaagt accatgagag ttcaggtagt cgattggggt
    32821 atcaacaccg tgcatcgcca tcttcatgca gcgcactgca ccttcgccag acggagcaac
    32881 catgtctgcg ccatcagagg ttgcgccgta gccaacgatt tcagcataga tgtgagcacc
    32941 acgcgccagc gcgtgttcca gctcttcaac cactaccata ccgccaccgc cagcgataac
    33001 gaaaccgtca cggtgagcgt cgtaagtacg ggaggctttt tccggggtgt cgttgtattt
    33061 agtagacagc gcacccatcg cgtcgaattc gcaagccatt tcccagcaca gctcttcgcc
    33121 gccgccagca aacacgatgt cctgtttgcc cagttggatc tgctctactg cgttaccgat
    33181 acagtgtgcg gaagtcgcac acgcggagct gatggagtag ttaacgccgt gaattttaaa
    33241 cggcgtggcg aggcaggcag aaacgccgga tgccatcgct ttggtgacca catacgggcc
    33301 aaccgctttc aggccgcgcg ggccgcgcat tgcgtcagcg ccgaaaacct ggaaacgcgg
    33361 ggagccgccg ccggaacctg caatcaggcc aacgcgcggg ttattctggt aagcttccgg
    33421 ggagaggcca gcatcagcaa ttgcctgctc catagaaagg aatgcataaa tggatgcgtc
    33481 gctcataaag cgcacaactt tgcggtcaat gaggccagtg gtatccagtt ttacgttgcc
    33541 ccagacgtgg ctacgcatgc cggaatcctt cagctcctga gagaaagtga tccctgaacg
    33601 tccttcacgc agagatgcca ggacttcctg ctggttatta ccgatgctgg aaacaatgcc
    33661 caggccagta atcactgcac gtttcattca atacctctgt aagtcgcaca tagagtaagt
    33721 ttcgaatgca caatagcgta cacttgtacg ccgaacaagt ccgatcagcc aattaataga
    33781 gaaatttgcg cagcctgaca cacatcgcta agatcgagcc accgcctgta agacgagtaa
    33841 cttacgtgaa acactactcc atacaacctg ccaacctcga atttaatgct gagggtacac
    33901 ctgtttcccg agattttgac gatgtctatt tttccaacga taacggactg gaagagacgc
    33961 gttatgtttt tctcgggggt aaccatttag aggcacgttt tcctgaacat ccacatcctc
    34021 tgtttgtggt agcagagagc ggcttcggca ccggattaaa cttcctgacg ctatggcagg
    34081 catttgatca gtttcgcgaa gcatatccgc aagcgcaatt acaacgctta catttcatta
    34141 gttttgagaa atttcccctc gcccgtgcgg atttagcgct ggcgcatcaa cactggccgg
    34201 agctggcccc gtgggcggaa cagcttcagg cgcaatggcc tttgcccttg cccggttgcc
    34261 atcgtttatt gctcgatgaa ggccgcataa cgctggattt atggtttggc gatataaacg
    34321 aactgaccag ccaactggac gattcgctaa atcaaaaagt agatgcctgg tttctggacg
    34381 gctttgcgcc agcgaaaaac ccggatatgt ggacgcaaaa tctgtttaac gccatggccc
    34441 ggctggcgcg tccgggcggc acgctggcga cctttacgtc tgccggtttt gtccgccgcg
    34501 gtttgcagga agccggattc actatgcaaa aacgtaaggg ctttgggcgc aaacgggaaa
    34561 tgctttgtgg ggtgatggaa ctgacattac ccctcccctg ctccacgccg tggtttaacc
    34621 gtacgggcag cagcaaacgg gaagcggcga ttatcggcgg tggtattgcc agcgcgttgt
    34681 tgtcgctggc gctattgcgt cgcggctggc aggtaacact ttattgcgcg gatgaggccc
    34741 ccgcactggg tgcttccggc aatcgccagg gggcgctgta tccgctttta agtaaacacg
    34801 atgaagcgct aaaccgtttt ttctctaatg cgtttacttt tgctcgtcgg ttttacgatt
    34861 tattgcccgt taaatttgat catgactggt gcggcgtcac gcagttaggc tgggatgaga
    34921 aaagccagca taaaatcgca cagatgttgt cgatggattt acccacagaa ctggctgtag
    34981 ccgttgaggc aaatgcggtt gaacaaatta cgggcgttgc gacaaattgc agcggtatta
    35041 cttatccgca aggtggctgg ctgtgtccag cagaactgac ccgtaatgtg ctggaactgg
    35101 cgcaacagca gggtttgcag attcattatc aatatcagtt acaggattta tcccgtaagg
    35161 atgacggttg gttgttgaat tttgcaggag atcagcaagc aacacacagt ttagtggtac
    35221 tggcgaacgg gcatcaaatc agccgtttca gccaaacgtc atctctgcca gtatattcgg
    35281 tggccggtca agtcagccat attccgacaa cgccggagct ggcgaaactg aagcaagtgc
    35341 tgtgctatga cggttatctc acgccgcaaa atccggcgaa tcagcaccat tgtattggtg
    35401 ccagttatca tcgcggcagc gaagagacgg cgtacagtga tgaagatcag cagcagaatc
    35461 gccagcggtt gattgattgt ttcccgcagg tacagtgggc aaaagaggtt gatgtcagtg
    35521 gtaaagaggc gcgctgcggt gtgcgttgtg ctactcgcga tcatctgcca atggtaggca
    35581 acgtgcctga ttatgacgct acactcgtgg aatatgcgtc gctggcggag aagaaagatg
    35641 aagcggtaag cgcgccggtt tatgacgatc tctttatgtt tgctgcttta ggttcgcgcg
    35701 gtttgtgttc tgccccgctg tgtgccgaga ttctggcggc acagatgagt gaagaaccga
    35761 ttccgatgga tgccagcacg ctggcggcgt taaacccgaa tcggttatgg gtgcggaagt
    35821 tgttgaaggg taaagcggtt aaggcggggt aatctgctct ggcattgttt gtcggatgcg
    35881 gcgtaaacgc cttatccgac ctacgcgtta cctgtaggcc tgataagacg cgataagcgt
    35941 cgcatcaggc atggtgctcc agacgtcaca acttactgct gtgaggcctg ctgaaacagg
    36001 tgttcccaca tatcggtcac cagcgcctgg tcacgcggcg acaattcacc ggcaccaatg
    36061 gctttttcca gactctggct aacggttgta tgtaccgcct gagcggagtg gtcgtcacca
    36121 ctttccagtt ctgcgatggc taatgtcagg tggccacgca aatacccact ggcaaacaac
    36181 tcatcatcac tggcatggtc aaccataccg tcgattaatg ccagaatgcg tgattcaaac
    36241 tccgcgatca tcttctttcc tcattgtaaa acgtggcgct ggcttcagtt gagcgcttcc
    36301 ggccacggga actgttccgc cgtaagttcc ggcgtgtgat aataattttg tagtgcttta
    36361 atgaagcgtg ccggacgttc gggaatgccg tttgccagat aatccatcac ctgcgcatgt
    36421 acccgccgtt gaaacaccac gcggtccggt tcgaaatccc cttccagatt gtcgcagcta
    36481 acattaaacg gatatcccgc tgccacgcag aacaaccagt ccagcgcctg cggcttcact
    36541 tcaacatctt caaactggct ttgtgtctgg gcatcgcgtc cgtccgggca ataccagtag
    36601 ccgaaatcaa ccagctcacg gcgcgctttc ccggcaatac accagtgcga aatctcgtgg
    36661 atggcgctgg cataaaagcc atgagcgaag acgattctgt tatacggtac ttccgcatca
    36721 gcaggaagat agatcggttc gtcgtcgcct ttaatcagac gggtattaaa ttcatcggca
    36781 aagcagctat taaaaatctc aatcaactgc tcatagtggt gtgtactgtt cattagttca
    36841 tccccaacca gtggaggatc tcctgtccat ggctatcgta aagtaatttg gcactcatca
    36901 ccgccgagac gataacgatc atcgggcgga tcagcttttg tcctttgctc aacaccagtc
    36961 ttgaacccat gcgcgcgccg aggaactgcc ccaccagcat cacaaagccc gtcgcccaaa
    37021 tcactttgcc gccgagaata aacagcagca gaccgccaat gtttgacgtt gcgttaagta
    37081 atttggcgtg agcagtggct ttggcgaggt tgaatccgca cagcgtaacg aaggccagcg
    37141 cgtaaaacga tccggcagcc gggccaaaaa atccatcgta aaaaccgacg cagccccccg
    37201 ccaccagcgc aaatggcagt ccatacatcc gacgctggcg atcttcttca cccagctttg
    37261 gcatcagcaa gaaatagagg ccgatacaaa tcaccagaat aggcaaaatc tgccgtaaga
    37321 catcggcctg aacatactgc accagtagcg cgccgctcat tgagccgaca aaggtcatgg
    37381 cgatattgag tttttgatcg cttaaactaa ccactttacg gcgaataaag tagatagtgg
    37441 cggaaataga gccaccgcag gcttgcagtt tattggttgc cagcgcgtta gcgggagaca
    37501 tccccgccgc catcaatgcc ggaatggtga gtaacccacc gccaccggca atcgagtcga
    37561 taaatcccgc cagcatggcg acaaaaaaga gaactcccag cagcagcggg gaaaccataa
    37621 acaggctatt aaacgtttcc attagatcac gtgctcatcc agtagcgcct ggcaggaagg
    37681 cggcaacgga ggcggtgtct tcttctcagg ctttgttgtt cccggttttg gaggttcaaa
    37741 ccagctttgc agttctgccc cgcaaccatc gcctggtggc ggtaaaggtt gatcctcaca
    37801 ctccagacta tcggcaggac aacgtaatcg tacatgcata tgcgcgcgat gctggaacca
    37861 gggtcgcact ttgcgcaacc agtcgcgatc ggtgcccgca tcaaggcaaa gttgttgctt
    37921 aatcgccgga ttaacaaaaa tgcgcgtgac gtctttatcc tgggcggcga gtttgatcaa
    37981 gctgaaaatt tctggcttcc acagcgtcgg gacaacgtgt ttaccgtcgc gggaaactaa
    38041 atctaacgct tgcgggcgca agagttgcgc ggaggtccag cgtgttttcg gcagttgcag
    38101 gaagatatcg acatccagtc cggtctggtg gctggcgtga ccgccgttga aacgaccacc
    38161 agcaggcatc cccatatcgc caatcagcac cgtacccatg cccagattgc tcacctggcg
    38221 actcagacgc tggataaaca tcaccaggtc tgggtgaccg aaatagcgac gctgatcggt
    38281 acgcatgacc tgataatgtt cggactgtat cggcaacgtg tcagcaccga tgatacagcc
    38341 attagaaaaa ctgcctatcg attgtgcgtt acccggcaca ggttgggtta ttttttgcca
    38401 cggcgtcgct gccaggctgg cgctactggc aagcagagcc agcagcgcaa tcgcggtttt
    38461 gttcattttt taccagcgtg gaatttcagt cttcacatcg gcattttgcg cccgctgccg
    38521 taacaggtga tccatcaaaa cgatcgccag catcgcttct gcgatcggca ccgcgcggat
    38581 cccaacacaa ggatcgtgac ggcctttggt gatcatctca acttcttcgc caaagcggtt
    38641 aatagtgcgc cccggcacgg taatgctgga ggtcggtttc agcgccatgt gggcaatgat
    38701 ttgctgcccg ctgctgatac caccaagaat gccgcccgca tggttgctct ggaaaccgtc
    38761 tttggtgatt tcgtcgcggt tctggctgcc acgcagcgct accacgtcaa aaccatcacc
    38821 aatttccacg cctttcaccg cgttaatgct cattagcgca tgggcgatgt cggcatccag
    38881 gcggtcaaag accggctcgc caagtccggc gggtacgcca ctggcaacga cggtgacttt
    38941 cgcaccaata gagtcgcctt cttttttcag cgcgcgcatc agttcatcta acgcgtcgat
    39001 tttgtccggg tccgggcaga agaatggatt ttgctcgacc tgagaccagt ctttgatttc
    39061 cagcggaatg tcgcccatct gggtcaggca accgcgaatc tcaatgccaa atttctcggc
    39121 gagatatttt ttggcaatcg cccctgctgc aacgcgcata gcggtttcac gggcggaaga
    39181 acgtccaccg ccgcgataat cacgcaggcc gtatttttgt tcgtaagtgt aatcggcatg
    39241 acctggacgg aaaacgtcct taatcgcact ataatcctga gaacgctggt cggtattttc
    39301 gatcaacaat ccgatgctgg tgccagtagt aacaccttca aaaacaccgg agagaatttt
    39361 gacctgatcc ggctcgcggc gctgggtggt atagcgcgat gtcccagggc gacgacgatc
    39421 aaggtcatgt tgcaggtccg cttccgtcag cggaatgcct ggcggaacac catcgacgat
    39481 gcagccgagc gccagcccgt gcgattcgcc gaaggtggtt acgcgaaaga gttgtccaat
    39541 tgtgtttcca gccatcacgg ctccgttatt gttgtgtttg cgtgtttact taatctttat
    39601 aaatcgcgaa atgttctcgt gcggcaataa gctgctcttt agtgagcata aacacaccat
    39661 cgccgccgtt atcaaactcc agccaggtga acggaacatc cggatattgt tccataagat
    39721 gtaccatgct gttgccgact tcacaaatca gcacgccatc atcagcgagg taatctgccg
    39781 cgtttccgag aatgcgacgc gtcagtttca ggccgtcggt gccagatgcc aggcccagtt
    39841 ccggctcgtg gcgatattcg tttggcaggt cggacatatc ttccgcatcg acatacggcg
    39901 ggttagtgac aatcaggtcg tactgcactt tcggcaagtc gcggaacaaa tcggaccgaa
    39961 taggaatgac gttgtggatc agaccgtgtt cttcgatgtt ctgttcagca accgccagcg
    40021 cgtcgggaga gatgtccacc gcgtcgactt ccgcttccgg gaaggcataa gcacaggcaa
    40081 tggcgatgca gccgctaccg gtacacatat ctaaaatgtg ctgcggttgc ttgctgatga
    40141 gtccggcaaa tttattgttg atcagttcac caatcggcga gcgcggcacc agcacgcgtt
    40201 catcgacgta aaattcatgg ccgcagaacc aggctctgtt ggtcaggtaa gccaccggaa
    40261 tgcgttcgtt gacgcggcgg atcacgcgct caacaatacg gtgtttttcg ctggaggtca
    40321 gacgcgcggt gcgcatgtct tccggaatat ccagcggcag gtagagtgaa ggcaacacca
    40381 gttgcacggc ttcatcccac gggttatcgg tgccgtggcc gtaccagata ttcgccgcgc
    40441 tgaagcggct caccgaccag cgcaacatgt cctgaatggt ttgcagctca tttactgctt
    40501 catcaacgaa aattttatcc acgtattcct ccagggcatg atcgcaataa tttcggcggc
    40561 tagtttgcca tgaagatgac gataaatcag cattcacgcg cggtgagtga ggaaaaatac
    40621 gtttaaaacg atcgattgcg ctacgagtcg ggtaaactgt aggaaaatta gaaatagaga
    40681 aagataaatg aaaaagaaaa caacacttag cgaggaggac caggcgctgt ttcgccagtt
    40741 gatggcgggt actcgcaaga ttaagcagga cacgattgtc caccgaccgc agcgtaaaaa
    40801 aatcagcgaa gtaccagtaa agcggttgat tcaggagcag gccgatgcca gccactattt
    40861 ttcggacgag tttcaaccat tattaaatac tgaagg
//
LOCUS       DAHHTG010000029        39707 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.029, whole genome shotgun sequence.
ACCESSION   DAHHTG010000029 DAHHTG010000000
VERSION     DAHHTG010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 39707)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39707)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39707
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.029"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            447..2732
                     /gene="nrdA"
                     /locus_tag="NBI74_001516"
     CDS             447..2732
                     /gene="nrdA"
                     /locus_tag="NBI74_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase subunit
                     alpha"
                     /protein_id="HCD1931876.1"
                     /translation="MNQNLLVTKRDGSTERINLDKIHRVLDWAAEGLHNVSISQVELR
                     SHIQFYDGIKTSDIHETIIKAAADLISRDAPDYQYLAARLAIFHLRKKAYGQFEPPAL
                     YDHVVKMVEMGKYDNHLLEDYTEEEFKQMDTFIDHDRDMTFSYAAVKQLEGKYLVQNR
                     VTGEIYESAQFLYILVAACLFSNYPRETRLQYVKRFYDAVSTFKISLPTPIMSGVRTP
                     TRQFSSCVLIECGDSLDSINATSSAIVKYVSQRAGIGINAGRIRALGSPIRGGEAFHT
                     GCIPFYKHFQTAVKSCSQGGVRGGAATLFYPMWHLEVESLLVLKNNRGVEGNRVRHMD
                     YGVQINKLMYTRLLKGEDITLFSPSDVPGLYDAFFADQEEFERLYTKYEKDDSIRKQR
                     VKAVELFSLMMQERASTGRIYIQNVDHCNTHSPFDPAIAPVRQSNLCLEIALPTKPLN
                     DVNDENGEIALCTLSAFNLGAINSLDELEELAILAVRALDALLDYQDYPIPAAKRGAM
                     GRRTLGIGVINFAYYLAKHGKRYSDGSANNLTHKTFEAIQYYLLKASNELAKEQGACP
                     WFNETTYAKGILPIDTYKKDLDAIANEPLHYDWEALRESIKTHGLRNSTLSALMPSET
                     SSQISNATNGIEPPRGYVSIKASKDGILRQVVPDYEHLHDAYELLWEMPGNDGYLQLV
                     GIMQKFIDQSISANTNYDPSRFPSGKVPMQQLLKDLLTAYKFGVKTLYYQNTRDGAED
                     AQDDLVPSIQDDGCESGACKI"
     gene            2821..3951
                     /gene="nrdB"
                     /locus_tag="NBI74_001517"
     CDS             2821..3951
                     /gene="nrdB"
                     /locus_tag="NBI74_001517"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     beta"
                     /protein_id="HCD1931877.1"
                     /translation="MAYTTFSQTKNDQLKEPMFFGQPVNVARYDQQKYDIFEKLIEKQ
                     LSFFWRPEEVDVSRDRIDYQALPEHEKHIFISNLKYQTLLDSIQGRSPNVALLPLISI
                     PELETWVETWAFSETIHSRSYTHIIRNIVNDPSVVFDDIVTNEQIQKRAEGISSYYDE
                     LIEMTSYWHLLGEGTHTVNGKTVTVSLRELKKKLYLCLMSVNALEAIRFYVSFACSFA
                     FAERELMEGNAKIIRLIARDEALHLTGTQHMLNLLRSGADDPEMAEIAEECKQECYDL
                     FVQAAQQEKDWADYLFRDGSMIGLNKDILCQYVEYITNIRMQAVGLDLPFQTRSNPIP
                     WINTWLVSDNVQVAPQEVEVSSYLVGQIDAEVDTDDLSNFQL"
     gene            3951..4205
                     /gene="yfaE"
                     /locus_tag="NBI74_001518"
     CDS             3951..4205
                     /gene="yfaE"
                     /locus_tag="NBI74_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like diferric-tyrosyl radical
                     cofactor maintenance protein YfaE"
                     /protein_id="HCD1931878.1"
                     /translation="MARVTLRITGTQLLCQDKHPSLLAALESHNVAVEYQCREGYCGS
                     CRTRLVAGQVDWIAEPLAFIQPGEILPCCCRAKGDIEIEM"
     gene            complement(4259..4909)
                     /gene="inaA"
                     /locus_tag="NBI74_001519"
     CDS             complement(4259..4909)
                     /gene="inaA"
                     /locus_tag="NBI74_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide kinase InaA"
                     /protein_id="HCD1931879.1"
                     /translation="MAVSAKHDEFNHWWATEGDWVEEPNYRRNGMSGVQCVERNGKKL
                     YVKRMTHHLFHSVRYPFGRPTIVREVAVIKELERAGVIVPKIVFGEAVKIEGEWRALL
                     VTEDMAGFISIADWYARHAVSPYSDEVRQAMLKAVALAFKKMHSVNRQHGCCYVRHIY
                     VKTEGKAEAGFLDLEKSRRRLRRDKAINHDFRQLEKYLEPIPKADWEQVKAYYYAM"
     gene            complement(5112..6188)
                     /gene="glpQ"
                     /locus_tag="NBI74_001520"
     CDS             complement(5112..6188)
                     /gene="glpQ"
                     /locus_tag="NBI74_001520"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HCD1931880.1"
                     /translation="MKLTLKNLSMAIMMSTIVMGSSAMAADSNEKIVIAHRGASGYLP
                     EHTLPAKAMAYAQGADYLEQDLVMTKDDHLVVLHDHYLDRVTDVADRFPDRARKDGRY
                     YAIDFTLDEIKSLKFTEGFDIENGKKVQTYPGRFPMGKSDFRVHTFEEEIEFVQGLNH
                     STGKNIGIYPEIKAPWFHHQEGKDIAAKTLEVLKKYGYTGKDDKVYLQCFDADELKRI
                     KNELEPKMGMDLNLVQLIAYTDWNETQQKQPDGSWVNYNYDWMFKPGAMKQVAEYADG
                     IGPDYHMLIEETSQPGNIKLTGMVQDAQQNKLVVHPYTVRSDKLPEYTTDVNQLYDVL
                     YNKAGVNGLFTDFPDKAVKFLNKE"
     gene            complement(6193..7551)
                     /gene="glpT"
                     /locus_tag="NBI74_001521"
     CDS             complement(6193..7551)
                     /gene="glpT"
                     /locus_tag="NBI74_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate transporter"
                     /protein_id="HCD1931881.1"
                     /translation="MLSIFKPAPHKARLPAAEIDPTYRRLRWQIFLGIFFGYAAYYLV
                     RKNFALAMPYLVEQGFSRGDLGFALSGISIAYGFSKFIMGSVSDRSNPRVFLPAGLIL
                     AAAVMLFMGFVPWATSSIAVMFVLLFLCGWFQGMGWPPCGRTMVHWWSQKERGGIVSV
                     WNCAHNVGGGIPPLLFLLGMAWFNDWHAALYMPAFCAILVALFAFAMMRDTPQSCGLP
                     PIEEYKNDYPDDYNEKAEQELTAKQIFMQYVLPNKLLWYIAIANVFVYLLRYGILDWS
                     PTYLKEVKHFALDKSSWAYFLYEYAGIPGTLLCGWMSDKVFRGNRGATGVFFMTLVTI
                     ATIVYWMNPAGNPTVDMICMIVIGFLIYGPVMLIGLHALELAPKKAAGTAAGFTGLFG
                     YLGGSVAASAIVGYTVDFFGWDGGFMVMIGGSILAVILLIVVMIGEKRRHEQLLQKRN
                     GG"
     gene            7824..9452
                     /gene="glpA"
                     /locus_tag="NBI74_001522"
     CDS             7824..9452
                     /gene="glpA"
                     /locus_tag="NBI74_001522"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit A"
                     /protein_id="HCD1931882.1"
                     /translation="MKTRDSQSSDVIIIGGGATGAGIARDCALRGLRVILVERHDIAT
                     GATGRNHGLLHSGARYAVTDAESARECISENQILKRIARHCVEPTNGLFITLPEDDLS
                     FQATFIRACEEAGISAEAIDPQQARIIEPAVNPALIGAVKVPDGTVDPFRLTAANMLD
                     AKEHGAVILTAHEVTGLIREGAAVCGVRVRNHLTGEIQALHAPVVVNAAGIWGQHIAE
                     YADLRIRMFPAKGSLLIMDHRINQHVINRCRKPSDADILVPGDTISLIGTTSLRIDYN
                     EIDDNRVTAEEVDILLREGEKLAPVMAKTRILRAYSGVRPLVASDDDPSGRNVSRGIV
                     LLDHAERDGLDGFITITGGKLMTYRLMAEWATDAVCRKLGNTRPCTTADLALPGSQEP
                     AEVTLRKVISLPAPLRGSAVYRHGDRTPAWLSEGRLHRSLVCECEAVTAGEVQYAVEN
                     LNVNSLLDLRRRTRVGMGTCQGELCACRAAGLLQRFNVTTSAQSIEQLSTFLNERWKG
                     VQPIAWGDALRESEFTRWVYQGLCGLEKEQKDAL"
     gene            9442..10701
                     /gene="glpB"
                     /locus_tag="NBI74_001523"
     CDS             9442..10701
                     /gene="glpB"
                     /locus_tag="NBI74_001523"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase subunit GlpB"
                     /protein_id="HCD1931883.1"
                     /translation="MRFDTVIMGGGLAGLLCGLQLQKHGLRCAIVTRGQSALHFSSGS
                     LDLLSHLPDGQPVTDIHSGLESLRQQAPAHPYTLLGPQRVLDLACQAQALIAESGAQL
                     QGSVELAHQRITPLGTLRSTWLSSPEVPVWPLPAKKICVVGISGLMDFQAHLAAASLR
                     ELDLKVETAEIELPELDVLRNNATEFRAVNIARFLDNEENWPLLLDALIPVANTCEMI
                     LMPACFGLADDKLWHWLNEKLPCSLMLLPTLPPSVLGIRLQNQLQRQFVRQGGVWMPG
                     DEVKKVTCKNGVVNEIWTRNHADIPLRPRFAVLASGSFFSGGLVAERDGIREPILGLD
                     VLQTATRGEWYKGDFFAPQPWQQFGVTTDEALRPSQAGQTIENLFAIGSVLGGFDPIA
                     QGCGGGVCAVSALHAAQQIAQRAGGQQ"
     gene            10698..11888
                     /gene="glpC"
                     /locus_tag="NBI74_001524"
     CDS             10698..11888
                     /gene="glpC"
                     /locus_tag="NBI74_001524"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit C"
                     /protein_id="HCD1931884.1"
                     /translation="MNDTSFENCIKCTVCTTACPVSRVNPGYPGPKQAGPDGERLRLK
                     DGALYDEALKYCINCKRCEVACPSDVKIGDIIQRARAKYDTTRPSLRNFVLSHTDLMG
                     SVSTPFAPIVNTATSLKPVRQLLDAALKIDHRRTLPKYSFGTFRRWYRSVAAQQAQYK
                     DQVAFFHGCFVNYNHPQLGKDLIKVLNAMDTGVQLLSKEKCCGVPLIANGFTDKARKQ
                     AITNVESIREAVGVKGIPVIATSSTCTFALRDEYPEVLNVDNKGLRDHIELATRWLWR
                     KLDEGKTLPLKPLPLKVVYHTPCHMEKMGWTLYTLELLRKIPGLELTVLDSQCCGIAG
                     TYGFKKENYPTSQAIGAPLFRQIEESGADLVVTDCETCKWQIEMSTSLRCEHPITLLA
                     QALA"
     gene            12081..13076
                     /locus_tag="NBI74_001525"
     CDS             12081..13076
                     /locus_tag="NBI74_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /protein_id="HCD1931885.1"
                     /translation="MTESTTSSPHDAVFKTFMFTPETARDFLEIHLPEPLRKLCNLQT
                     LRLEPTSFIEKSLRAYYSDVLWSVETSEGDGYIYCVIEHQSSAEKNMAFRLMRYATAA
                     MQRHLDKGYDRVPLVVPLLFYHGEASPYPYSLNWLDEFDDPQLARQLYTEAFPLVDIT
                     IVPDDEIMQHRRIALLELIQKHIRDRDLIGMVDRITTLLVRDFTNDSQLQTLFNYLLQ
                     CGDTSRFTRFIQEIAERSPLQKERLMTIAERLRQEGHQIGWQEGKIEGWQEGKLEGLQ
                     QGKLEGLQKGKLEGLQEGMHEQAIKIALRMLEQGFEREIVLAATQLTDADIPNCH"
     gene            13077..13334
                     /locus_tag="NBI74_001526"
                     /pseudo
     CDS             13077..13334
                     /locus_tag="NBI74_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708134.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(13375..14178)
                     /gene="yfaU"
                     /locus_tag="NBI74_001527"
     CDS             complement(13375..14178)
                     /gene="yfaU"
                     /locus_tag="NBI74_001527"
                     /EC_number="4.1.2.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxy-L-rhamnonate aldolase"
                     /protein_id="HCD1931886.1"
                     /translation="MNALLTNPFKERLRKGEVQIGLWLSSTTAYMAEIAATSGYDWLL
                     IDGEHAPNTIQDLYHQLQAVAPYASQPVIRPVEGSKPLIKQVLDIGAQTLLIPMVDTA
                     DQARQVVSATRYPPYGERGVGASVARAARWGRIENYMAQVNDSLCLLVQVESKTALDN
                     LDEILDVEGIDGVFIGPADLSASLGYPDNAGHPEVQRIIETSIRRIRAAGKAAGFLAV
                     APDMAQQCLAWGANFVAVGVDTMLYSDALDQRLAMFKSGKNGPRVKGSY"
     gene            complement(14196..15485)
                     /locus_tag="NBI74_001528"
     CDS             complement(14196..15485)
                     /locus_tag="NBI74_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416749.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCD1931887.1"
                     /translation="MSTALLDAVVKKNRARLIPFMLALYVLAFLDRSNIGFAKQTYQI
                     DTGLSNEAYALGAGIFFVVYAFLGVPANLLMRKLGARTWIGTTTLLWGFLSAAMAWAD
                     TEAKFLIVRTLLGAAEAGFFPGMIYLTSQWFPQRNRASIMGLFYMGAPLALTLGSPLS
                     GALLEMHGFMGHPGWFWMFVIEGLLAVGAGVFTFFWLDDTPGQARFLSKQEKTLLINQ
                     LASEEQQKVTSRLSDALRNGRVWQLAIIYLTIQVAVYGLIFFLPTQVAALLGTKVGFT
                     ASVVTAIPWVAALFGTWLIPRYSDKTGERRNVAALTLLAAGIGIGLSGLLSPVLAIVA
                     LCVAAIGFIAVQPVFWTMPTQLLSGTALAAGIGFVNLFGAVGGFIAPILRVKAETLFS
                     SDAAGLLTLAAVAVIGSLIIFTLRVNRTVAQTDVAHH"
     gene            complement(15542..16747)
                     /gene="rhmD"
                     /locus_tag="NBI74_001529"
     CDS             complement(15542..16747)
                     /gene="rhmD"
                     /locus_tag="NBI74_001529"
                     /EC_number="4.2.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311162.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnonate dehydratase"
                     /protein_id="HCD1931888.1"
                     /translation="MTLPKIKQVRAWFTGGATAEKGAGGGDYHDQGANHWIDDHIATP
                     MSKYREYEQSRQSFGINVLGTLIVEVEAENGQTGFAVSTAGEMGCFIVEKHLNRFIEG
                     KCVSDIKLIHDQMLNATLYYSGSGGLVMNTISCVDLALWDLFGKVVGLPVYKLLGGAV
                     RDEIQFYATGARPDLAKEMGFIGGKMPTHWGPHDGDAGIRKDAAMVADMREKCGEDFW
                     LMLDCWMSQDVNYATKLAHACAPYNLKWIEECLPPQQYEGYRELKHNAPAGMMVTSGE
                     HHGTLQSFRTLSETGIDIMQPDVGWCGGLTTLVEIAAIAKSRGQLVVPHGSSVYSHHA
                     VITFTNTPFSEFLMTSPDCSTMRPQFDPILLNEPVPVNGRIHKSVLDKPGFGVELNRD
                     CNLKRPYSH"
     gene            complement(16762..17544)
                     /locus_tag="NBI74_001530"
     CDS             complement(16762..17544)
                     /locus_tag="NBI74_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HCD1931889.1"
                     /translation="MLESSKVPALTRAIDILNLIARIGPCSAATIIDTLGIPKSTAYL
                     LLNELRRQRFLSLDHQENFCLWTRLVELSGHALSKMDLRELARPRLTQLMDTTGLLCH
                     LGIIDNGSAYYILKVESSATISVRSHEGKSLSLYRSGIGKCLLAWQPAAVQQSIIEGL
                     VWERATPTTITHPQRLYEELARIRRQGWSYDNGEDYADVRCVAAPVFNANNELTAAIS
                     VVGTRLQINEEYRDYLAGKAIACARDISRLLGWKSPFDLQAS"
     gene            complement(17764..18966)
                     /locus_tag="NBI74_001531"
     CDS             complement(17764..18966)
                     /locus_tag="NBI74_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide deamidase-related
                     protein YfaY"
                     /protein_id="HCD1931890.1"
                     /translation="MLKVEMLSTGDEVLHGQIVDTNAAWLADFFFHQGLPLSRRNTVG
                     DNLDDLVTILRERSQHADVLIVNGGLGPTSDDLSALAAATAKGEGLVLHEAWLKEMER
                     YFHERGRVMAPSNRKQAELPASAEFINNPVGTACGFAIQLNRCLMFFTPGVPSEFKVM
                     VEHEILPRLRERFSLPQPPVCLRLTTFGRSESDLAQSLDTLQLPPGVTMGYRSSMPII
                     ELKLTGPASEEQAMEKLWLDVKRVAGQSVIFEGTEGLPAQISRELQSRQFSLTLSEQF
                     TGGLLALQLSRAGAPLLACEVVPSQEETLAQTAHWITERRANHFAGLALAVSGLEDEH
                     LNFALATPDGTFALRVHFSATRYSLAIRQEVCAMMALNMLRRWLNGQDIASEHGWIEV
                     VESMTLSV"
     gene            complement(19066..19608)
                     /locus_tag="NBI74_001532"
     CDS             complement(19066..19608)
                     /locus_tag="NBI74_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1931891.1"
                     /translation="MKKIALAGLAGMLLVSASVNAMSISGQAGKEYTNIGVGFGTEST
                     GLALSGNWTHNDDDGDAAGVGLGLNLPLGPLMATVGGKGVYTNPNDGDEGYAAAVGGG
                     LQWQIGDSFRLFGEYYYSPDSLSSGIKSYEEANAGARYTIMRPVSIEAGYRYLNLAGK
                     NGNRDNAIADGPYVGVNASF"
     gene            19887..20312
                     /gene="nudI"
                     /locus_tag="NBI74_001533"
     CDS             19887..20312
                     /gene="nudI"
                     /locus_tag="NBI74_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphatase NudI"
                     /protein_id="HCD1931892.1"
                     /translation="MRQRTIVCPLIQNDGAYLLCKMADDRGVFPGQWALSGGGVEPGE
                     RIEEALRREIREELGEQLLLTEITPWTFSDDIRTKTYADGRKEEIYMIYLIFDCVSAN
                     RDVKINEEFQDYAWVKPEDLVHYDLNVATRKTLRLKGLL"
     gene            complement(20351..20953)
                     /locus_tag="NBI74_001534"
     CDS             complement(20351..20953)
                     /locus_tag="NBI74_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HCD1931893.1"
                     /translation="MLAFCRSSLKSKKYFIILLALAAIAGLGTHAAWSSNGLPRIDNK
                     TLARLAQQHPVVVLFRHAERCDRSTNQCLSDKTGITVKGTQDARELGNAFSADIPDFD
                     LYSSNTVRTIQSATWFSAGKKLTVDKRFLQCGNEIYSAIKDLQRKAPDKNIVIFTHNH
                     CLTYIAKDKRDATFKPDYLDGLVMHVEKGKVYLDGEFVNH"
     gene            21261..22400
                     /gene="arnB"
                     /locus_tag="NBI74_001535"
     CDS             21261..22400
                     /gene="arnB"
                     /locus_tag="NBI74_001535"
                     /EC_number="2.6.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_024188371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-4-amino-4-deoxy-L-arabinose
                     aminotransferase"
                     /protein_id="HCD1931894.1"
                     /translation="MSGFLPFSRPAMGVEELAAVKEVLESGWITTGPKNQALEQAFCQ
                     LTGNQHAIAVSSATAGMHITLMALEIGKGDEVITPSLTWVSTLNMISLLGATPVMVDV
                     DRDTLMVTPEAIEAAITPRTKAIIPVHYAGAPADIDAIRAIGERYGIAVIEDAAHAVG
                     TYYKGRHIGAKGTAIFSFHAIKNITCAEGGLIVTDNENLARQLRMLKFHGLGVDAYDR
                     HTWGRAPQAEVLTPGYKYNLTDINAAIALTQLVKLEHLNTRRREIAQQYQQALATLPF
                     QPLSLPAWPHVHAWHLFIIRVDEQRCGISRDALMEALKERGIGTGLHFRAAHTQKYYR
                     ERFPTLSLPNTEWNSERICSLPLFPDMTTADADRVITALQQLAGQ"
     gene            22404..23372
                     /gene="arnC"
                     /locus_tag="NBI74_001536"
     CDS             22404..23372
                     /gene="arnC"
                     /locus_tag="NBI74_001536"
                     /EC_number="2.4.2.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate
                     4-deoxy-4-formamido-L-arabinose transferase"
                     /protein_id="HCD1931895.1"
                     /translation="MFEIHPVKKVSVVIPVYNEQESLPELIRRTTAACESLGKEYEIL
                     LIDDGSSDNSAHVLVEASQAEGSHIVSILLNRNYGQHSAIMAGFSHVTGDLIITLDAD
                     LQNPPEEIPRLVAKADEGYDVVGTVRQNRQDSWFRKTASKMINRLIQRTTGKAMGDYG
                     CMLRAYRRHIVDAMLHCHERSTFIPILANIFARRAIEIPVHHAEREFGESKYSFMRLI
                     NLMYDLVTCLTTTPLRMLSLLGSIIAIGGFSIAVLLVILRLTFGPQWAAEGVFMLFAV
                     LFTFIGAQFIGMGLLGEYIGRIYTDVRARPRYFVQQVIRPSSKENE"
     gene            23372..25354
                     /gene="arnA"
                     /locus_tag="NBI74_001537"
     CDS             23372..25354
                     /gene="arnA"
                     /locus_tag="NBI74_001537"
                     /EC_number="1.1.1.305"
                     /EC_number="2.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-4-amino-4-deoxy-L-arabinose
                     formyltransferase/UDP-glucuronic acid oxidase ArnA"
                     /protein_id="HCD1931896.1"
                     /translation="MKTVVFAYHDMGCLGIEALLSAGYEISAIFTHTDNPGEKAFYGS
                     VARLAAERGIPVYAPDNVNHPLWVERIAQLSPEVIFSFYYRHLICDEILQLAPRGAFN
                     LHGSLLPKYRGRAPLNWVLVNGETETGVTLHRMVKRADAGAIVAQLRVAIAPDDIAIT
                     LHHKLCHAARQLLEQTLPAIKHGNILEIAQRENEATCFGRRTPDDSFLEWHKPASVLH
                     NMVRAVADPWPGAFSYVGNQKFTVWSSRVHPHASKALPGSVISVAPLLIACGDGALEI
                     VTGQAGDGITMQGSQLAQTLGLVQGSRLNSQPACAARRRTRVLILGVNGFIGNHLTER
                     LLREDHYEVYGLDIGSDAISRFLNHPHFHFVEGDISIHSEWIEYHVKKCDVVLPLVAI
                     ATPIEYTRNPLRVFELDFEENLRIIRYCVKYRKRIIFPSTSEVYGMCSDKYFDEDHSN
                     LIVGPVNKPRWIYSVSKQLLDRVIWAYGEKEGLQFTLFRPFNWMGPRLDNLNAARIGS
                     SRAITQLILNLVEGSPIKLIDGGKQKRCFTDIRDGIEALYRIIENAGNRCDGEIINIG
                     NPENEASIEELGEMLLASFEKHPLRHYFPPFAGFRVVESSSYYGKGYQDVEHRKPSIR
                     NARRCLDWEPKIDMQETIDETLDFFLRTVDLTDKPS"
     gene            25351..26241
                     /locus_tag="NBI74_001538"
     CDS             25351..26241
                     /locus_tag="NBI74_001538"
                     /EC_number="3.5.1.n3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000169712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-deoxy-4-formamido-L-arabinose-
                     phosphoundecaprenol deformylase"
                     /protein_id="HCD1931897.1"
                     /translation="MTKVGLRIDVDTFRGTREGVPRLLEILSKHNIQASIFFSVGPDN
                     MGRHLWRLVKPQFLWKMLRSNAASLYGWDILLAGTAWPGKEIGHANADIIREAAKHHE
                     VGLHAWDHHAWQAHSGNWDRQTMIDDIARGLRTLEEIIGQPVTCSAAAGWRADQQVIE
                     AKEAFHLRYNSDCRGAMPFRPLLESGTPGTAQIPVTLPTWDEVIGRDVKAEDFNGWLL
                     NRIQRDKGTPVYTIHAEVEGCAYQHNFVDLLKRAAQEGVTFCPLSELLSGTLPLGQVV
                     RGNIAGREGWLGCQQMAGSH"
     gene            26241..27893
                     /gene="arnT"
                     /locus_tag="NBI74_001539"
     CDS             26241..27893
                     /gene="arnT"
                     /locus_tag="NBI74_001539"
                     /EC_number="2.4.2.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A)
                     4-amino-4-deoxy-L-arabinosyltransferase"
                     /protein_id="HCD1931898.1"
                     /translation="MKSVRYLIGLFAFIACYYLLPISTRLLWQPDETRYAEISREMLA
                     SGDWIVPHLLGLRYFEKPIAGYWINSIGQWLFGANNFGVRAGVIFATLLTAALMTWFT
                     LRLWRDKRLALLATVIYLSLFIVYAIGTYAVLDPFIAFWLVAGMCSFWLAMQAQTWKG
                     KSAGFLLLGITCGMGVMTKGFLALAVPVLSVLPWVATQKRWKDLFIYGWLAVISCVLT
                     VLPWGLAIAQREPDFWHYFFWVEHIQRFALDDAQHRAPFWYYLPVIIAGSLPWLGLLP
                     GALYAGWKNRKHSATVYLLSWTIMPLLFFSVAKGKLPTYILSCFAPLAMLMAHYALLA
                     AKNNPLALRINGWINIAFGVTGIIATFVVSPWGPMNTPVWQTFESYKVFCAWSIFSLW
                     AFFGWYTLTNVEKTWPFAALCPLGLALLVGFSIPDRVMEGKHPQFFVEMTQESLQPSR
                     YILTDSVGVAAGLAWSLQRDDIIMYRQTGELKYGLNYPDAKGRFVSGDEFANWLNQHR
                     QEGIITLVLSVDRDEDINSLAIPPADAIDRQGRLVLIQYRPK"
     gene            27890..28225
                     /gene="arnE"
                     /locus_tag="NBI74_001540"
     CDS             27890..28225
                     /gene="arnE"
                     /locus_tag="NBI74_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnE"
                     /protein_id="HCD1931899.1"
                     /translation="MIWLTLVFASLLSVAGQLCQKQATCFAAVNKRRKHIVLWLGLAL
                     ACLGLAMVLWLLVLQNVPVGIAYPMLSLNFVWVTLAAVKLWHEPVSLRHWCGVAFIIG
                     GIVILGSTV"
     gene            28225..28611
                     /gene="arnF"
                     /locus_tag="NBI74_001541"
     CDS             28225..28611
                     /gene="arnF"
                     /locus_tag="NBI74_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416761.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnF"
                     /protein_id="HCD1931900.1"
                     /translation="MGLMWGLFSVIIASAAQLSLGFAASHLPPMTHLWDFIAALLAFG
                     LDARILLLGLLGYLLSVFCWYKTLHKLALSKAYALLSMSYVLVWIASMVLPGWEGTFS
                     LKALLGVACIMSGLMLIFLPTTKQRY"
     gene            complement(28605..28871)
                     /gene="pmrD"
                     /locus_tag="NBI74_001542"
     CDS             complement(28605..28871)
                     /gene="pmrD"
                     /locus_tag="NBI74_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311174.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction protein PmrD"
                     /protein_id="HCD1931901.1"
                     /translation="MEWLVKKSCCNKQNNRHVLMLCDAGGAIKMIAEVKSDFAVKVGD
                     LLSPLQNALYCINREKLHTVKVLSASCYSPDEWERQCKAAGKTQ"
     gene            complement(28981..30336)
                     /gene="menE"
                     /locus_tag="NBI74_001543"
     CDS             complement(28981..30336)
                     /gene="menE"
                     /locus_tag="NBI74_001543"
                     /EC_number="6.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000577608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate--CoA ligase"
                     /protein_id="HCD1931902.1"
                     /translation="MIFSDWPWRHWRQVRGEAIALRLNDEQLNWRELCARVDELASSF
                     AAQGVVAGSGVMLRAWNTPQTLLAWLALLQCGARVLPVNPQLPQPLLEELLPNLTLQF
                     ALVPEGENTFPALTSLHIQQVEGVHAVAWQPTRLCSMTLTSGSTGLPKAAVHTYQAHL
                     ASAEGVLSLIPFGDHDDWLLSLPLFHVSGQGIMWRWLYAGARMTVRDKQPLEQMLAGC
                     THASLVPTQLWRLLVNRSSVSLKAVLLGGAAIPVELTEQAREQGIRCFCGYGLTEFAS
                     TVCAKEADGLADVGSPLPGREVKIVNDEVWLRAASMAEGYWRNGQRVPLVNDEGWYAT
                     RDRGEMHNGKLTIVGRLDNLFFSGGEGIQPEEVERVIAAHPAVLQVFIVPVADKEFGH
                     RPVAVVEYDQQTVDLGEWVKDKLARFQQPVRWLTLPPELKNGGIKISRQALKEWVQRQ
                     D"
     gene            complement(30333..31295)
                     /gene="menC"
                     /locus_tag="NBI74_001544"
     CDS             complement(30333..31295)
                     /gene="menC"
                     /locus_tag="NBI74_001544"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HCD1931903.1"
                     /translation="MRSAQVYRWQIPMDAGVVLRDRRLKTRDGLYVCLREGEREGWGE
                     ISPLPGFSQETWEDAQSVLLAWVNNWLAGDCELPQMPSVAFGVSCALAELADTLPQAA
                     NYRAAPLCNGDPDDLILKLADMPGEKVAKVKVGLYEAVRDGMVVNLLLEAIPDLHLRL
                     DANRAWTPLKGQQFAKYVNPDYRHRIAFLEEPCKTRDDSRAFARETGIAIAWDESLRE
                     PDFAFVAEEGVRAVVIKPTLTGSLEKVREQVKAAHALGLTAVISSSIESSLGLMQLAR
                     IAAWLTPDTIPGLDTLDLMQVQQVRRWPGSPLPLVDVDALERLL"
     gene            complement(31295..32152)
                     /gene="menB"
                     /locus_tag="NBI74_001545"
     CDS             complement(31295..32152)
                     /gene="menB"
                     /locus_tag="NBI74_001545"
                     /EC_number="4.1.3.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001641973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA synthase"
                     /protein_id="HCD1931904.1"
                     /translation="MIYPDEAMLYAPVEWHDCSEGFEDIRYEKSTDGIAKITINRPQV
                     RNAFRPLTVKEMIQALADARYDDNIGVIILTGAGDKAFCSGGDQKVRGDYGGYKDDSG
                     VHHLNVLDFQRQIRTCPKPVVAMVAGYSIGGGHVLHMMCDLTIAADNAIFGQTGPKVG
                     SFDGGWGASYMARIVGQKKAREIWFLCRQYDAKQALDMGLVNTVVPLADLEKETVRWC
                     REMLQNSPMALRCLKAALNADCDGQAGLQELAGNATMLFYMTEEGQEGRNAFNQKRQP
                     DFSKFKRNP"
     gene            complement(32167..32925)
                     /gene="menH"
                     /locus_tag="NBI74_001546"
     CDS             complement(32167..32925)
                     /gene="menH"
                     /locus_tag="NBI74_001546"
                     /EC_number="4.2.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-6-hydroxy-2,
                     4-cyclohexadiene-1-carboxylate synthase"
                     /protein_id="HCD1931905.1"
                     /translation="MILHAQAKHGKPGLPWLVFLHGFSGDCHEWQEVGEAFADYSRLY
                     VDLPGHGGSATISVDGFDDVTDLLCKTLVSYNILNFWLVGYSLGGRVAMMAACQELAG
                     LCGVVVEGGHPGLQNAEQRAERQRSDRQWAQRFRTEPLTAVFADWYQQPVFASLNDDQ
                     RRELVALRSNNNGATLAAMLEATSLAVQPDLRANLSARTFAFYYLCGERDSKFRALAA
                     ELAADCHVIPRAGHNAHRENPAGVIASLAQILRF"
     gene            complement(32922..34592)
                     /gene="menD"
                     /locus_tag="NBI74_001547"
     CDS             complement(32922..34592)
                     /gene="menD"
                     /locus_tag="NBI74_001547"
                     /EC_number="2.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-5-enolpyruvyl-6-hydroxy-3-
                     cyclohexene-1-carboxylic-acid synthase"
                     /protein_id="HCD1931906.1"
                     /translation="MSVSAFNRRWAAVILEALTRHGVRHICIAPGSRSTPLTLAAAEN
                     SAFIHHTHFDERGLGHLALGLAKVSKQPVAVIVTSGTAVANLYPALIEAGLTGEKLIL
                     LTADRPPELIDCGANQAIRQPGMFASHPTHSISLPRPTQDIPARWLVSTIDHALGTLH
                     AGGVHINCPFAEPLYGEMDDTGISWQQRLGDWWQDDKPWLREAPRRESEKQRDWFFWR
                     QKRGVVVAGRMSAEEGKKVALWAQTLGWPLIGDVLSQTGQPLPCADLWLGNAKATSEL
                     QQAQIVVQLGSSLTGKRLLQWQASCEPEEYWIVDDIEGRLDPAHHRGRRLIANIADWL
                     ELHPAEKRQPWCVEIPRLAEQAMQAVIARRDAFGEAQLAHRISDYLPEQGQLFVGNSL
                     VVRLIDALSQLPAGYPVYSNRGASGIDGLLSTAAGVQRASGKPTLAIVGDLSALYDLN
                     ALALLRQVSAPLVLIVVNNNGGQIFSLLPTPKSERERFYLMPQNVHFEHAAAMFELKY
                     HRPQNWQELETTLVDAWRTPTTTVIEMVVNDTDGAQTLQQLLAQVSHL"
     gene            complement(34681..35976)
                     /gene="menF"
                     /locus_tag="NBI74_001548"
     CDS             complement(34681..35976)
                     /gene="menF"
                     /locus_tag="NBI74_001548"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase MenF"
                     /protein_id="HCD1931907.1"
                     /translation="MQSLTTALENLLRHLSQEIPATPGIRVIDIPFPLKDAFDALSWL
                     ASQQVYPQFYWQQRNGDEEAAVLGAITRFTSLDQAQRFLRQHPEHADLRIWGLNAFDP
                     SQGYLLLPRLEWRRCGGKATLRLTLFSESSLQHDAIKAKEFIATLVSIKPLPGLHLTT
                     TREQHWPDKTGWTLLIELATKTIAEGELDKVVLARATDLHFASPVNAAAMMAASRRLN
                     LNCYHFYMAFDGENAFLGSSPERLWRRRDKALRTEALAGTVANHPDDKQAQQLGEWLM
                     ADDKNQRENMLVVEDICQRLQADTRTLDVLPPQVLRLRKVQHLRRCIWTSLNKADDVI
                     CLHQLQPTAAVAGLPRDLARQFIARHEPFTREWYAGSAGYLSLQQSEFCVSLRSAKIS
                     GNIVRLYAGAGIVRGSDPEQEWQEIDNKAAGLRTLLQME"
     gene            complement(36055..36360)
                     /gene="elaB"
                     /locus_tag="NBI74_001549"
     CDS             complement(36055..36360)
                     /gene="elaB"
                     /locus_tag="NBI74_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein ElaB"
                     /protein_id="HCD1931908.1"
                     /translation="MSNQFGDTRIDDDLTLLSETLEEVLRSSGDPADQKYVELKARAE
                     KALDDVKKRVSQASDSYYYRAKQAVYRADDYVHEKPWQGIGVGAAVGLVLGLLLARR"
     gene            complement(36415..36876)
                     /locus_tag="NBI74_001550"
     CDS             complement(36415..36876)
                     /locus_tag="NBI74_001550"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCD1931909.1"
                     /translation="MIDWQDLHHSDLSVSQLYALLQLRCAVFVVEQNCPYQDIDGDDL
                     EGENRHILGWHNGTLVAYARILKSDDELQPVVIGRVIVSEALRGEKIGQQLMSKALES
                     CTRRWPEKPIYLGAQAHLQNFYGQFGFIPVTDIYEEDGIPHIGMAREVIQA"
     gene            36941..37858
                     /gene="rbn"
                     /locus_tag="NBI74_001551"
     CDS             36941..37858
                     /gene="rbn"
                     /locus_tag="NBI74_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease BN"
                     /protein_id="HCD1931910.1"
                     /translation="MELIFLGTSAGVPTRTRNVTAILLNLQHPTQSGLWLFDCGEGTQ
                     HQLLHTAFNPGKLDKIFISHLHGDHLFGLPGLLCSRSMSGIIQPLTIYGPHGIREFVE
                     TALRISGSWTDYPLEIVEISTGEIFDDGLRKVTAYPMEHPLECYGYRIEEHDKPGALN
                     AQALKAAGVPPGPLFQELKAGKTIMLDDGRQINGADYLAAPVPGKALAIFGDTGPCDA
                     ALELAKGVDVMVHEATLDMAMEAKANSRGHSSTRQAAALAREAGVGKLIITHVSSRYD
                     DKGCQHLLRECRSIFPATELANDFAVFSI"
     gene            complement(37979..>39707)
                     /locus_tag="NBI74_001552"
     CDS             complement(37979..>39707)
                     /locus_tag="NBI74_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001244797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HCD1931911.1"
                     /translation="IIMLLISSLILSGCGPEPEDKKSQQQQSTTPTDQQVLAAQQAAT
                     KVAEQSAAKAAADAKALAQQEVQQYSDKQALLGRLQAAPKYQHAAREKAASQIANPAT
                     ARYQQFDDNPVKQVAQNPLATFSLDVDTGSYANVRRFLNQGLLPPPDAVRVEEIVNYF
                     PSDWDIKDKQSIPASKPIPFAMRYELAPAPWNEQRTLLKVDILAKDRKSEELPASNLV
                     FLIDTSGSMISDERLPLIQSSLKLLVKELREQDNIAIVTYAGDSRIALPSISGSHKAE
                     INAAIDSLDAEGSTNGGAGLEMAYQQAAKGFIKGGINRILLATDGDFNVGIDDPKSIE
                     SMVKKQRESGVTLSTLGVGDSNYNEAMMVRIADVGNGNYSYIDTLSEAQKVLNSEMRQ
                     TLITVAKDVKAQIEFNPAWVTEYRQIGYEKRQLKAEDFNNDNVDAGDIGAGKHITLLF
                     ELTLKGQKASIDKLRYAPDNKSAKSDKTKELAWLKIRWKSPQGKESQLVEFPLASAIK
                     APSEDMRFRAAVAAYGQKLRGSEYLNNTSWQQIKQWAQKAKGEDPQGYRAEFIRLIGL
                     AKDLDNSQN"
BASE COUNT         9674 a        10634 c         9868 g         9531 t
ORIGIN      
        1 ttagtattta acaataaccg aatataaaac aaccatttcc gactcaacaa tcgcttacat
       61 tcgcttatat attgaccaca acagatacat cagattatgt gacgacttgt gcctagatca
      121 attttggcaa tcattagcaa caagattaat aaaccatcta tatcaattta cctatcctat
      181 tttaccgttc aagaattcac cgaacggtta tttttaacaa atttttctct tcccattgac
      241 tttcccggac accttgtctg acctaaggtg cgcggaagcc actttttcct tcctgagtta
      301 tccacaaagt tatgcacttg caagagggcc agtttcacac tatcttgcag tgaatcccaa
      361 acataccccc tatatatagt gttcttagca gcttccctca ctacaggtag tctgcatgaa
      421 gctattgaaa aacaggtacg acatacatga atcagaatct gctggtgaca aagcgcgacg
      481 gtagcacaga gcgcatcaat ctcgacaaaa tccatcgcgt tctggattgg gcggcagaag
      541 gactgcataa cgtttcgatt tcccaggtcg agctgcgctc ccacattcag ttttatgacg
      601 gtatcaagac ctctgacatc cacgaaacca ttatcaaggc tgccgcagac ctgatctccc
      661 gtgatgcgcc ggattatcag tatctcgccg cacgtctggc gatcttccac ctgcgtaaaa
      721 aagcctacgg tcagtttgag ccgcctgcgc tgtacgacca tgtggtgaaa atggtcgaga
      781 tgggcaaata cgataatcat ctgctggaag actacacgga agaagagttc aagcagatgg
      841 acacgtttat cgatcacgac cgtgatatga ccttctctta tgctgccgtt aagcagctgg
      901 aaggcaaata tctggtacag aaccgcgtga ccggcgaaat ctacgaaagc gcccagttcc
      961 tttatattct ggttgccgcg tgcctgttct cgaactaccc gcgtgaaacg cgcctgcaat
     1021 acgtgaagcg tttttacgac gcggtttcca catttaaaat ttcgctgccg acgccaatca
     1081 tgtccggcgt gcgtaccccg actcgtcagt tcagctcatg cgtgctgatc gagtgcggcg
     1141 acagcctgga ttccattaac gccacctcca gcgcgattgt taaatacgtt tcccagcgtg
     1201 ccgggatcgg catcaacgcc gggcgtattc gtgcgctggg tagcccgatt cgcggtggcg
     1261 aagcgttcca taccggttgt attccgttct acaaacattt ccagacagcg gtgaaatcct
     1321 gctctcaggg cggtgtgcgc ggcggtgcgg caacgctgtt ctacccgatg tggcatctgg
     1381 aagtggaaag cctgctggtg ttgaaaaaca accgtggtgt ggaaggcaac cgcgtgcgtc
     1441 atatggacta cggggttcaa atcaacaaac tgatgtatac ccgtctgctg aaaggtgaag
     1501 atatcactct gttcagcccg tccgacgtac cggggctgta cgacgcgttc ttcgccgatc
     1561 aggaagagtt tgaacgtctg tataccaaat atgagaaaga cgacagcatc cgcaagcagc
     1621 gtgtgaaagc cgttgagctg ttctcgctga tgatgcagga acgtgcgtct accggtcgta
     1681 tctatattca gaacgttgac cactgcaata cccatagccc gtttgatccg gccatcgcgc
     1741 cagtgcgcca gtctaacctg tgcctggaaa tcgccctgcc gaccaaaccg ctgaacgacg
     1801 tcaacgacga gaacggtgaa atcgcactgt gtacgctgtc tgctttcaac ctgggcgcaa
     1861 ttaacagcct ggatgaactg gaagagctgg caattctggc agtacgcgca ctcgacgcgc
     1921 tgctggatta tcaggattac ccgatcccgg ccgccaaacg tggagcgatg ggtcgtcgta
     1981 cgctgggtat tggtgtgatc aacttcgctt actacctggc gaagcacggt aaacgctact
     2041 ccgacggcag cgccaacaac ctgacgcata aaaccttcga agccattcag tattacctgc
     2101 tgaaagcgtc taatgagctg gcgaaagagc aaggcgcgtg cccgtggttt aacgaaacca
     2161 catacgcgaa agggatcctg ccgatcgata cctataagaa agatctggat gccatcgcta
     2221 atgagccgct gcattacgac tgggaagcac tgcgtgagtc aatcaaaacg cacggcctgc
     2281 gtaactccac gctttctgcc ctgatgccgt ccgagacttc ttcgcagatc tctaacgcca
     2341 ctaacggtat tgaaccgccg cgcggttacg tcagcatcaa agcgtcgaaa gacggtattc
     2401 tgcgccaggt ggtgccggac tacgagcatc tgcacgacgc ctatgagctg ttgtgggaaa
     2461 tgccgggtaa cgatggctac ctgcaactgg tgggtatcat gcagaaattt atcgatcagt
     2521 cgatctctgc taacaccaac tacgatccgt cacgtttccc gtcaggaaaa gtgccgatgc
     2581 agcagttgct gaaagacctg ctcactgcct acaaattcgg ggtcaaaacg ctgtattatc
     2641 agaacacccg tgacggcgca gaagacgcgc aggacgatct ggtgccgtca atccaggacg
     2701 atggctgcga aagcggcgca tgtaagatct gataatcccc cagtctttgc accaaaccgt
     2761 cccctctccc cttgcgggag aggggacggg tgaggggtaa taaaaccaca ggacacattc
     2821 atggcatata ccaccttttc acagacgaaa aatgatcagc tcaaagaacc aatgttcttt
     2881 ggtcagccgg tcaacgtggc tcgctacgat cagcaaaaat atgacatctt cgaaaagctg
     2941 atcgaaaagc agctctcttt cttctggcgt ccggaagaag ttgacgtctc ccgcgaccgt
     3001 atcgattacc aggcactgcc tgaacacgaa aaacacatct ttatcagcaa cctgaaatat
     3061 cagacgctgc tggattccat tcagggtcgc agtccgaacg tggcactgct gccgctgatc
     3121 tcgatcccgg aactggaaac ctgggtagaa acctgggcgt tctcagaaac gattcattcc
     3181 cgctcctata cccatatcat ccgtaatatc gttaacgatc cgtctgttgt ctttgacgat
     3241 atcgttacca acgagcagat ccagaaacgt gcggagggga tctccagcta ttacgatgaa
     3301 ctgatcgaaa tgaccagcta ctggcatctg ctgggcgaag gcacccacac cgttaacggt
     3361 aaaaccgtaa ccgttagcct gcgcgagctg aagaaaaaac tgtatctctg cctgatgagc
     3421 gttaacgcgc tggaagcgat ccgcttctac gtcagctttg cctgctcttt cgcattcgct
     3481 gaacgtgagc tgatggaagg taacgccaaa attattcgcc tgattgcccg cgacgaagcc
     3541 ctgcacctga ccggcaccca gcatatgctg aatctgctgc gcagcggcgc ggatgatccg
     3601 gagatggcgg aaattgccga agaatgtaag caggagtgct atgacctgtt tgtccaggcg
     3661 gcacagcaag agaaagactg ggcggattat ctgttccgtg atggttcgat gattggtctg
     3721 aataaagaca ttctctgcca gtacgttgaa tacatcacca atatccgtat gcaggcagtc
     3781 ggtttggatc tgccgttcca gacgcgctcc aacccgatcc cgtggatcaa cacctggctg
     3841 gtgtctgata acgtgcaggt tgctccgcag gaagtggaag tcagttctta tctggtcggg
     3901 cagattgacg cggaagtgga caccgacgat ttgagtaact tccagctctg atggcccgcg
     3961 ttaccctgcg cattactggc acacaactgc tgtgccagga taaacaccct tcccttctgg
     4021 cggcgctgga atcccacaat gtggcggttg agtaccagtg ccgcgaaggt tactgcggct
     4081 cctgtcgcac ccgactggtc gcaggccagg tggactggat tgccgaaccg ttagctttta
     4141 ttcagccggg ggaaattttg ccctgttgtt gccgggcaaa aggcgatatt gaaatcgaga
     4201 tgtgaattgg ttttaacgcc agatacgacg cttatacgtc ttatctggcc tacaacgatt
     4261 acatagcgta gtaatatgct ttcacctgct cccagtccgc tttggggatc ggctccagat
     4321 atttttccag ctggcggaag tcatgattaa tcgctttatc acgacgcaag cggcgacgac
     4381 ttttctccag gtcaagaaat ccagcttctg ccttaccttc tgttttcaca tagatatggc
     4441 gaacataaca acagccatgc tgacgattaa cgctatgcat tttcttaaac gccagcgcca
     4501 ctgctttcaa catggcttgc cgtacttcgt cagaataagg cgatactgca tgccgggcat
     4561 accagtcagc aatgctgatg aaccccgcca tatcttcagt caccagcaac gcgcgccatt
     4621 caccctcaat tttcaccgct tcaccaaaaa caatcttcgg cacgatgaca ccggcccgtt
     4681 ccagttcttt aattaccgca acctcacgga caatcgttgg tcggccgaac ggataacgta
     4741 cggaatgaaa cagatgatgc gtcatgcgct ttacatacag ctttttgccg ttgcgctcga
     4801 cgcattgcac cccgctcata ccattacggc gatagttagg ctcttcaacc cagtcgccct
     4861 ctgttgccca ccagtgatta aattcgtcgt gttttgctga aactgccata ccctatcgcc
     4921 tgtcgttttt tattaacgac aatgactata gatgtttagc tgaggaaaat cttaataata
     4981 cgtgttgtat tgacgagtat cttatgccgg gaacaagtca tctcgctcta aataattgca
     5041 attaaggata acccacagac tttaatgaag agtggtatcg gatagcacat aagtgccatc
     5101 cggtagttaa tttactcttt attaaggaat tttactgcct tatcagggaa gtcagtaaat
     5161 agcccattta cacccgcttt gttatacaga acatcatata actgattcac atcagtagtg
     5221 tattcaggca gtttatctga ccgcacggta taaggatgca ctaccagttt attctgctga
     5281 gcatcttgca ccatgccagt gagtttgata ttacccggct gcgatgtctc ctcaatcaac
     5341 atatggtaat ccggaccaat accatcagca tattccgcca cctgtttcat ggcacccggc
     5401 ttaaacatcc agtcgtaatt gtaattaacc cagcttccgt ccggctgttt ctgctgcgtt
     5461 tcattccagt cggtataggc aatcagctgt accagattga gatccatacc cattttaggt
     5521 tccagctcat tcttaatacg cttcagctca tcagcatcaa aacattgcaa ataaactttg
     5581 tcgtccttac cggtgtaacc atatttcttc agcacttcca gcgtttttgc cgcaatatcc
     5641 ttcccttcct gatgatggaa ccacggcgct ttaatttctg gatagatacc gatatttttc
     5701 ccggtagagt ggtttaaccc ctgaacaaat tcaatctctt cttcaaaggt gtgcacccgg
     5761 aagtcagatt tacccattgg gaaacgcccc ggataagtct gcactttttt accgttttca
     5821 atatcgaaac cttcggtaaa tttcagcgac ttaatttcat ccagcgtgaa atctatcgcg
     5881 tagtaacgac cgtctttgcg cgcccgatcc gggaaacgat cggcaacatc agtaacacga
     5941 tcgagataat ggtcatgcag aacaaccaga tggtcgtctt tggtcatcac caaatcctgt
     6001 tccagataat ccgctccctg cgcatacgcc atcgcttttg ctggcagcgt atgctccggc
     6061 aaatatccac tggcaccgcg atgggcgatg actatttttt cgttgctgtc cgccgccatt
     6121 gcactgcttc ccatgactat agtgctcatc atgatcgcca tgctaaggtt tttcagcgtc
     6181 aatttcatgc cattagcctc cgttgcgttt ttgcagtaat tgttcatggc gacgtttttc
     6241 gccaatcatc acaacaatca acaagataac cgccagaatg ctgccgccaa tcattaccat
     6301 aaagccgcca tcccagccga agaagtccac ggtgtagcca acaatcgcgc tcgccgccac
     6361 cgaaccgccc aggtaaccaa acagcccggt aaaacccgct gccgtacctg ccgctttttt
     6421 cggtgccagt tccagcgcat gtagaccgat cagcatcaca gggccataga tcaggaagcc
     6481 gataacaatc atacaaatca tatcgacggt tgggttacct gccgggttca tccagtaaac
     6541 gatagtcgcg atggtcacca gtgtcataaa gaagacgccg gttgccccac ggttaccacg
     6601 aaagacttta tccgacatcc agccgcacag cagggtgccc ggaatacctg catattcata
     6661 aagaaagtag gcccaggagg atttatccag cgcgaaatgc ttaacctctt tcagataagt
     6721 cggtgaccag tcgaggatgc cgtaacgcag cagataaacg aacacgttgg cgatggcgat
     6781 ataccacagc agtttgttcg gcagcacgta ctgcatgaag atctgcttcg cggtcagctc
     6841 ctgttccgct ttttcgttat agtcgtccgg ataatcattt ttgtactctt caatcggcgg
     6901 caggccacag gattgcgggg tatcgcgcat catcgcaaag gcgaacagtg ccaccagaat
     6961 ggcgcagaaa gcgggcatat agagcgccgc gtgccagtca ttgaaccagg ccatccccag
     7021 caggaacagc agcggcggaa taccaccacc gacgttgtgc gcacagttcc acactgacac
     7081 aataccgcca cgttctttct gcgaccacca gtgcaccata gtacgaccac acggcggcca
     7141 ccccatcccc tggaaccaac cgcagaggaa caacagtaca aacatcaccg caatgctcga
     7201 cgtcgcccat ggcacaaagc ccataaacaa catcactgcc gccgccagaa tcaaacctgc
     7261 gggcaggaaa acgcgcggat tcgagcgatc cgataccgaa cccatgatga atttcgaaaa
     7321 tccataagca atcgagatcc ccgaaagggc aaaacctaaa tcaccgcgtg agaatccctg
     7381 ctcaaccaga taaggcatag cgagcgcaaa gttcttacga accaaatagt aagcagcata
     7441 gccaaagaat atccccagga aaatttgcca gcgcaatcga cgataagtcg gatcgatctc
     7501 cgcggcaggt aagcgcgctt tgtgtggcgc tggtttaaaa atactcaaca ttgatagcct
     7561 ccgtggcccg tggtcttatt tatgattaac agcctgattc agtgggagaa cctgccgttt
     7621 cttgagttgc cgcgatgtta agaaaacatt cataaattaa atgtgaatta ccgcacacat
     7681 tattaaatac gatggttgaa atgttcagaa tgacgcatga aatcacgttt cactttcgaa
     7741 ttatgagcga atatgcgcga aatcaaacaa ttcatgtttt tactatggct aaatggtaaa
     7801 aaacgaactt ctgagggata acaatgaaaa ctcgcgactc gcaatcaagt gacgtgatta
     7861 tcattggcgg tggcgcaacg ggagccggga ttgcccgcga ctgtgccctg cgcgggctgc
     7921 gcgtgatttt ggttgagcgc cacgacatcg caaccggtgc caccgggcgt aaccacggcc
     7981 tgctgcacag cggtgcgcgc tatgcggtaa ccgatgcgga atcggcccgc gaatgtatta
     8041 gtgaaaacca gatcctgaaa cgcattgcac gtcactgcgt agaaccaacc aacggcctgt
     8101 ttatcaccct gccggaagat gacctctcct tccaggccac ttttatccgc gcctgcgaag
     8161 aagcagggat cagcgcagaa gccattgatc cgcagcaagc gcgcattatc gaacctgccg
     8221 ttaacccggc actgattggc gcggtgaaag ttccggatgg caccgttgat ccatttcgtc
     8281 tgacagcagc aaacatgctg gatgccaaag aacacggtgc cgttatcctt accgctcatg
     8341 aagtcacggg gctgattcgt gaaggcgcgg cggtgtgcgg cgttcgtgta cgtaaccatc
     8401 tcaccggcga aattcaggca cttcatgcac ctgtcgtagt taatgccgcc gggatctggg
     8461 ggcaacacat tgccgaatat gccgatctgc gcattcgcat gttcccggcg aaaggatcgc
     8521 tgctgatcat ggatcaccgc attaatcagc atgtgatcaa ccgctgccgc aaaccttccg
     8581 acgccgatat tctggtgcct ggcgatacca tttcgctgat tggcaccacc tctttacgta
     8641 ttgattacaa cgagattgac gataatcgag taacggcaga agaagttgat attctgctgc
     8701 gtgaagggga aaaactggcc cccgtgatgg cgaaaacgcg cattttgcgg gcctactctg
     8761 gcgtacgccc gctggttgcc agcgatgacg atccgagcgg acgtaacgtc agccgtggca
     8821 tcgtgctgct cgaccatgct gaacgcgatg gtctggacgg gtttatcacc atcaccggtg
     8881 gcaaactgat gacctatcgg ctgatggcgg aatgggctac cgatgccgta tgccgcaaac
     8941 tgggcaacac gcgcccctgt acgaccgccg atctggcact tcctggttca caggaacccg
     9001 ctgaagttac cttgcgtaaa gtcatctccc tacctgcccc gctgcgcggt tctgcggttt
     9061 atcgtcatgg cgatcgcacg cctgcctggc tgagcgaagg ccgtctgcac cgtagcctgg
     9121 tatgtgagtg cgaagcggta actgcgggtg aagtgcagta cgcggtagaa aatttaaacg
     9181 ttaatagcct gctggattta cgccgtcgta cccgtgtggg gatgggcacc tgccagggcg
     9241 agctctgcgc ctgccgcgct gccggactgc tgcaacgttt taacgtcacg acgtccgcgc
     9301 aatctattga gcaactttcc accttcctaa acgaacgctg gaaaggcgtg caacccatcg
     9361 cctggggaga tgcactgcgc gaaagcgaat ttacccgctg ggtttatcag ggattgtgtg
     9421 gtctggagaa ggagcagaaa gatgcgcttt gatactgtca ttatgggcgg cggcctcgcc
     9481 ggattactct gtggcctgca actgcaaaaa cacggcctgc gctgtgccat tgtcactcgt
     9541 ggtcaaagcg cgctccattt ctcatctgga tcgctggatt tgctgagcca tctgccggat
     9601 ggtcaaccgg tgacagacat tcacagtgga ctggaatctt tgcgtcagca ggcacctgcc
     9661 catccttaca ctcttctcgg gcctcaacgc gtgctcgatc tcgcttgcca ggcgcaggca
     9721 ttaatcgctg aaagcggtgc gcaattgcag ggcagcgtag aactggctca ccagcggatt
     9781 acgccgctcg gcactctgcg ctcaacctgg ctaagttcgc cagaagtccc tgtctggccg
     9841 ctgcccgcga agaaaatatg tgtagtggga attagcggcc tgatggattt tcaggcgcat
     9901 cttgcggcgg cttcattgcg cgaattggat cttaaagttg aaaccgctga aattgaactg
     9961 ccggaactgg atgtgctgcg caataacgcc accgagttcc gggcggtgaa tattgcccgc
    10021 ttccttgata atgaagaaaa ctggccgttg ttacttgatg cgcttattcc ggtcgccaat
    10081 acctgcgaaa tgatcctgat gcccgcctgc ttcggcctgg ccgatgacaa actgtggcat
    10141 tggttgaatg aaaaactacc ttgttcattg atgcttttgc caacgctgcc gccttccgtg
    10201 ctgggcattc gtctgcaaaa ccagttacag cgccagtttg tgcgccaggg cggcgtgtgg
    10261 atgccgggcg atgaagtgaa aaaagtgacc tgtaaaaatg gtgtagtgaa tgaaatctgg
    10321 acgcgcaatc acgccgatat cccgctgcgc ccacgatttg cggtgcttgc gagcggcagt
    10381 ttctttagcg gcggactggt agcggaacgt gacggcattc gcgagccgat tctcggcctt
    10441 gatgtgctac aaaccgctac gcggggtgaa tggtataagg gcgatttttt tgcgccgcag
    10501 ccgtggcagc agttcggtgt aaccactgat gaggcactac gcccgtcgca ggcagggcaa
    10561 accattgaga acctgtttgc catcggttcg gtgctgggcg gatttgatcc catcgcccag
    10621 ggatgcggcg gcggtgtttg tgccgtcagt gctttacatg ccgctcaaca gattgcccaa
    10681 cgcgcaggag gccaacaatg aacgacacca gcttcgaaaa ctgcattaag tgcaccgtct
    10741 gcaccaccgc ctgcccggtg agccgggtga atccgggtta tccggggcca aaacaagccg
    10801 ggccggatgg cgagcgtctg cgtttgaaag atggcgcact gtatgacgag gcgctgaaat
    10861 attgcatcaa ctgcaaacgt tgtgaagtcg cctgcccgtc cgatgtgaag attggcgata
    10921 ttatccagcg cgcgcgggcg aaatatgaca ccacgcgccc gtcgctgcgt aattttgtgt
    10981 tgagtcatac cgacctgatg ggtagcgtat ccacgccgtt cgcacccatc gtcaacaccg
    11041 ctacctcgct gaaaccggtg cgtcagctgc ttgatgcggc gttaaaaatc gatcatcgcc
    11101 gcacgctacc gaaatactcc ttcggcacgt tccgtcgctg gtatcgcagc gtggcggctc
    11161 agcaagcgca gtataaagac caggtcgctt tctttcacgg ctgcttcgtt aactacaacc
    11221 atccgcaact aggcaaagat ttaattaaag tgctcaacgc gatggatacc ggcgtacaac
    11281 tgctcagtaa agaaaaatgc tgcggcgtac cgctgatcgc caacggtttt accgataaag
    11341 cacgcaaaca ggcaattacg aatgtagagt cgatccgcga agctgtggga gtaaaaggca
    11401 ttccggtgat tgccacctcc tcaacgtgta catttgccct gcgcgacgaa tacccagaag
    11461 tgctgaatgt cgacaacaaa ggcttgcgcg atcatatcga actggcgacc cgctggctgt
    11521 ggcgcaagct ggacgaaggc aaaacgttac cgctgaaacc gctgccgctg aaagtggttt
    11581 atcacactcc gtgccatatg gaaaaaatgg gctggacgct ctacaccctg gagctgttgc
    11641 gtaaaatccc ggggcttgag ttaacggtgc tggattccca gtgctgcggt attgcgggaa
    11701 cttacggttt caaaaaagag aactacccca cttcacaagc catcggcgca ccactgttcc
    11761 gccagataga agagagcggc gcagatctgg tggtcaccga ctgcgaaacc tgcaaatggc
    11821 agattgagat gtccacaagt cttcgctgcg aacatccgat tacgctgctg gcacaggcgt
    11881 tggcttaaac tcctttctga tgcccggtaa gcatgttgtt accgggcatt tttgcgtaca
    11941 cgattccgtg cccaatgtat gcgttgcaac gcagtgaaaa ttcctctgaa aacgtctcgc
    12001 aaaggctgaa actggcagat gtcaaaggcc tgggataacc gtaatgtcgc gtcatcataa
    12061 atatcaggtg acggacaacc atgaccgaat caacaacctc ctccccgcat gatgcggtgt
    12121 ttaaaacctt tatgttcaca cccgaaaccg cacgggattt tctggaaata catctaccag
    12181 agccgctgcg caagctttgc aacctgcaaa ccttacgcct ggaacccacc agttttattg
    12241 aaaaaagttt acgcgcttac tactcggatg ttttgtggtc cgtggaaacc agcgagggtg
    12301 acggctatat ctactgcgtg attgaacatc aaagctctgc agaaaagaat atggcttttc
    12361 ggctaatgcg ctatgccact gccgccatgc agcgtcacct ggataaaggc tatgacagag
    12421 ttccgctggt ggtgccattg ctgttttatc atggcgaagc ctcgccctac ccgtactcac
    12481 tcaactggct ggatgagttt gacgatccgc aacttgcccg gcagttgtac accgaagctt
    12541 ttccgttggt ggatatcacc atcgtacctg acgatgagat catgcaacat cggcgtatag
    12601 ctctgctgga actgattcaa aagcatattc gcgaccgcga tttaatcggt atggtcgaca
    12661 ggatcaccac gcttttggtt agagacttca ctaatgacag ccagctacaa acactgttta
    12721 attatctgct gcaatgcggc gatacctccc gtttcacccg ctttattcag gagattgccg
    12781 aacgttcacc actacaaaag gagagattaa tgactattgc tgaacggcta cggcaggaag
    12841 gacatcaaat tggctggcaa gaaggtaaaa ttgaaggctg gcaagaaggt aaattagaag
    12901 gtttgcagca aggtaaattg gagggtttgc agaaaggtaa attagaaggt ttgcaggaag
    12961 gcatgcatga gcaagccatt aaaattgcct tgcgcatgct ggagcagggc tttgaacgtg
    13021 agattgtgct ggcggcaacc caactcactg atgctgatat tccgaactgt cattaaaagg
    13081 agagattgat gactattgct gaacggctac ggcaggaagg acatcaaatt ggctggcagg
    13141 agggtaagat tgaaggctgg caagaaggta aattagaagg tttgcaagaa ggtaaattag
    13201 aaggtttgca ggaaggcatg catgaacaag ccattaaaat tgctttgcgc atgctggaac
    13261 agggcattga tcgtgatcag gtgctcgcgg ccactcagct aagcgaagcc gatctggcag
    13321 cgaataacca ctaattaaca caggcccaca gccgatcccc atgggccttt gatatcaata
    13381 actacctttt acgcgtggcc catttttgcc tgatttaaac atcgccagtc gttgatccag
    13441 ggcatcgctg tagagcatcg tgtcaacgcc aacagcgaca aagttcgctc cccacgccag
    13501 gcattgctgc gccatatcag gagccacagc cagaaaacca gccgctttac ccgcagcacg
    13561 gatccgccga atgctggttt caataattcg ctgcacttcc gggtgcccgg cgttatccgg
    13621 gtagcccaac gacgcagaaa gatccgcagg tccaataaac acgccatcaa tcccttcgac
    13681 atcgaggatt tcgtccaggt tatccagtgc cgttttactt tccacctgca ccaacagaca
    13741 aagcgaatcg ttgacttgcg ccatgtaatt ctcaatgcgt ccccatcgcg cagcccgtgc
    13801 cacactggcc ccaacaccac gctcaccgta gggaggatag cgcgtggcag acaccacctg
    13861 acgtgcctga tcggcagtat cgaccatcgg gatcagtaga gtttgcgcgc caatatccag
    13921 gacttgttta atcagcggct tactgccttc caccggacgg atcacgggtt ggctggcata
    13981 gggcgctacc gcctgtagct ggtgataaag atcctgaatg gtgtttggcg catgctcccc
    14041 gtcaatcagc aaccagtcat aaccggaagt ggcggcaatt tctgccatat aggcagtcgt
    14101 tgagcttaac cacagaccaa tttgcacttc gcccttgcgt aaacgttctt taaagggatt
    14161 ggttaataat gcgttcatcg tgttccttaa actatttaat gatgtgccac gtcggtctgc
    14221 gcaacagtgc gatttacacg cagagtgaaa ataatcagcg aaccgatgac cgccaccgct
    14281 gccagcgtca gtaatcccgc cgcatcactg gaaaataacg tttctgcttt cacgcgcagg
    14341 atcggggcaa taaacccgcc cactgcacca aacaggttta caaaaccaat ccccgcagcc
    14401 agcgccgtac cggaaagaag ttgtgtcggc atcgtccaga acaccggttg cacggcaata
    14461 aacccgatgg ctgcaacaca cagtgctacg atcgccagta ctggagaaag cagcccggag
    14521 agaccaatac caatgcccgc cgccagtaat gtcagcgctg cgacattacg ccgttcgccg
    14581 gttttatcgg agtagcgcgg aataagccag gtcccaaaca aggccgcaac ccacggaatg
    14641 gcggtgacca ccgacgccgt aaagcccact tttgtcccca gcaatgcagc aacctgggtc
    14701 ggcaggaaga aaattaatcc gtaaaccgcc acttgaatgg tcaggtagat aatcgccagt
    14761 tgccagactc gcccattacg cagcgcatcg ctcaatcgag aagtcacttt ctgttgttct
    14821 tcacttgcca gttgattgat aagcaacgtt ttttcttgtt tactcagaaa acgtgcctgc
    14881 cccggtgtgt catcaagcca aaagaatgtg aataccccag cgccgactgc cagcaatcct
    14941 tcaatcacaa acatccagaa ccagccggga tgccccataa atccatgcat ctccagcagc
    15001 gcgccggaaa gcggtgatcc cagtgttaac gccagcggtg cgcccatata gaacagcccc
    15061 ataatgctgg cgcgattacg ctgcggaaac cattgcgaag tgagataaat cataccaggg
    15121 aaaaacccgg cctccgcagc accaagcaga gtgcgaacga tcagaaattt cgcttcagta
    15181 tcggcccatg ccatggcagc cgaaagaaat ccccacagca gtgttgtcgt accaatccag
    15241 gttctggccc ccagtttgcg catcaaaaga ttcgccggaa cccccagaaa cgcgtatacc
    15301 acaaagaaaa tgcctgctcc cagcgcataa gcttcattac tcagcccggt atcaatctgg
    15361 taggtctgtt tggcaaaacc aatattcgaa cggtcgagaa acgccagcac atacagcgcc
    15421 aacataaacg gaattaaacg cgcgcggttt ttcttcacca cggcgtcaag caaagcggtg
    15481 ctcataaaaa gctccttaga atatgtgggc gttaccgttg tcacagcaac gcccggagtg
    15541 attagtggct gtaggggcgt ttcaaattgc agtcacgatt gagttcgacg ccgaaaccgg
    15601 gtttatcaag cactgattta tgaatacgac cattcaccgg tactggctca ttaagcagaa
    15661 tcggatcaaa ttgcggacgc atcgttgaac aatccgggct ggtcatcagg aattcgctga
    15721 atggcgtatt ggtgaaggtg atcaccgcat ggtgggagta aacagacgaa ccgtgtggca
    15781 ccaccagttg ccccctggat ttggcgattg cggcaatttc caccagcgtg gttaagccac
    15841 cgcaccagcc aacatccggc tgcataatgt cgataccggt ttctgaaagc gtacggaaag
    15901 attgcagtgt gccgtggtgc tcaccgctgg tgaccatcat ccccgctggt gcgttgtgtt
    15961 tcagttcgcg ataaccttca tactgctgtg gcggcaggca ctcttcgatc catttcaggt
    16021 tatagggcgc gcaagcgtgg gccagtttgg tggcataatt cacgtcctga ctcatccagc
    16081 agtcgagcat taaccagaaa tcctcaccgc atttttcacg catatccgcg accatagcgg
    16141 catctttgcg gatccccgca tcgccatcat gtggccccca gtgcgtcggc attttgccac
    16201 cgataaagcc catctctttt gccagatccg gacgcgcacc tgtggcgtag aactgaatct
    16261 catcacgaac agcgccgcct aaaagtttat aaaccggaag cccgaccact ttgccgaaca
    16321 gatcccacag agccagatcg acacaggaaa tcgtattcat caccaggcca ccagagccgg
    16381 agtaatacag ggtggcattg agcatttgat cgtggatcag tttgatatca ctgacgcatt
    16441 taccctcaat gaaacggtta agatgttttt cgacaataaa acagcccatt tcaccggctg
    16501 tcgaaacagc gaatccggtc tggccgtttt ctgcttcaac ttcaacaatc aacgtgccaa
    16561 gaacgttaat gccaaatgac tggcgggact gctcgtattc gcggtattta ctcatcgggg
    16621 tggcaatatg atcatcaatc cagtgattcg ccccctggtc gtgataatca ccgccgccag
    16681 cgcctttttc tgctgtcgca ccgccagtaa accaggcgcg aacctgtttg atttttggta
    16741 gggtcatgat gttctccatt gttatgaggc ctgtaagtca aagggacttt tccatcccaa
    16801 caaacgtgaa atatccctgg cacaggcaat ggccttgccc gccagataat cacggtattc
    16861 ttcattgatt tgtaagcggg taccgaccac cgaaatcgca gcggtaagct cgttattggc
    16921 gttaaacacc ggcgcagcga cacaacggac atcggcgtaa tcttcgccgt tgtcatagct
    16981 ccagccctgg cggcgaatac gcgccagttc ttcgtagagc cgttgtggat gagtaatcgt
    17041 tgtgggtgtc gcccgttccc agaccagccc ttcgataata ctttgctgta cagctgcagg
    17101 ttgccaggca agcaggcatt tacctattcc ggagcgataa agcgaaaggc tttttccttc
    17161 atgggaacgc acgctgatag tggctgatga ctccactttc aaaatgtaat aagcgctgcc
    17221 gttatcaata atccccaggt gacataacag cccggtggta tccatcagtt gcgtcaaacg
    17281 cggtcgagcc agttcccgga gatccatctt gcttaacgca tgaccggaaa gctccaccag
    17341 cctggtccac aaacaaaaat tttcctggtg atcaaggcta agaaaacgct gacgcctgag
    17401 ttcattaagc agcaaatagg ctgtgctttt agggatcccc aatgtgtcaa tgatcgtcgc
    17461 agcactacag gggccaatcc gggcaatcaa attgagaata tcaatagccc gtgtcagggc
    17521 gggaactttg cttgattcca acatactgga ctccagtctt agatactgga atcagtctcg
    17581 ttgttcaggg ggagtaaaat tgtgaagcgc atcaaatccc gtttctcgtc cagcggactg
    17641 gagaaattac gcacaaattt gtgaatcagc gcacggattt tttcttattg agtacaacag
    17701 gttggacaaa ttttgccgga gggggaatgt gccggtgcag taaaccggca cagagtggcg
    17761 gaatcagaca gataaggtca tggactcaac aacctcaatc cagccatgct cgctggcgat
    17821 gtcctggccg tttaaccaac ggcgtagcat attaagcgcc atcattgcgc acacttcctg
    17881 gcgaatagcc agactgtagc gtgtggcact gaaatgtaca cgcaaagcga aagtgccgtc
    17941 tggcgtggct agtgcaaagt tgagatgctc atcttccaga ccagaaacag ccagtgccaa
    18001 cccagcaaaa tggttcgccc gccgttctgt aatccagtgc gcagtttgcg ccagggtttc
    18061 ctcctgtgaa ggaaccactt cacacgccag caatggagca cctgcgcgag aaagttgcaa
    18121 agccaataaa ccaccggtga attgctcgct cagcgtcagg ctgaactggc ggctctgcaa
    18181 ttcgcgactg atctgcgcgg gcagtccttc ggtgccttcg aaaatcacgc tctgtccggc
    18241 aacacgttta acgtccagcc acagtttttc catcgcctgt tcctcgcttg ccggtccggt
    18301 gagtttcagt tcgataatcg gcattgagga gcgatagccc attgttacgc ccggcggcag
    18361 ttgtagagtg tccaggcttt gtgccagatc gctttccgaa cgaccaaagg tagtcaaacg
    18421 caggcaaacc ggcggctgtg gtaaagaaaa acgctcgcgc aggcgcggca ggatttcgtg
    18481 ctcgaccatc accttaaatt ctgacggtac gccgggggta aagaacatca ggcaacgatt
    18541 aagctgtatg gcaaaaccac aggcagtgcc taccgggtta ttgataaatt cagcactggc
    18601 aggcagctcc gcttgtttac ggttgctcgg tgccattact cgtccccgtt catggaaata
    18661 gcgttccatc tctttgagcc aggcttcatg cagcaccagg ccttcacctt ttgctgtcgc
    18721 agcggcgagt gcgcttaaat catcactggt cggtcccagc ccgccgttaa cgatcaacac
    18781 atcggcgtgc tgactacgtt cgcgaagaat ggtgactaag tcatcaaggt tatcccccac
    18841 cgtattgcgg cgagataatg gcagcccctg atgaaagaaa aaatcggcca gccaggcagc
    18901 gttagtgtca acgatttgcc cgtgtaacac ttcatccccg gtggataaca tttccacttt
    18961 taacatggtt atctcctgct tttgttatcg aatcactata acgcaaaggg agacagggcg
    19021 tagtaagaaa ctgaatggcg cgacagaacg ccgcgccgga aggcgtcaga agctggcatt
    19081 taccccaaca tacggaccat cagcaatggc gttatcacga ttgccatttt tacctgccag
    19141 gttcaggtaa cggtagccag cctcaatgct gactggacgc ataatggtgt aacgcgcgcc
    19201 agcattcgct tcttcataac ttttaattcc gctggagagc gaatccggag agtagtaata
    19261 ctcaccaaac aagcggaagc tgtcaccaat ttgccactgc aaaccaccgc caactgccgc
    19321 tgcataacct tcatcaccat cgttcgggtt ggtgtaaacg ccttttccgc caacggtcgc
    19381 cattaatggc ccgagaggca gattcaaccc cagccccacg cccgcggcat caccgtcgtc
    19441 gtcgttatgt gtccagttac cgcttaacgc caggcccgtc gattcagtcc caaaaccgac
    19501 accaatattg gtgtattctt tacccgcctg gccgctgatg ctcattgcat tgaccgatgc
    19561 agaaaccaac agcattccgg ccagacctgc tagcgcaatt tttttcatta tcctctttcc
    19621 cacaagcaat taatcaaaat tcccaaagct gcgggattgt actgcctggc gcagaggatt
    19681 caatgagcga aaaaagggat ctagtcactt attgtaacta aatacttctt aaataattaa
    19741 acgccgaaaa aataaacaca ctttatcgat acacacaaaa ataataataa atgtagttat
    19801 catcgcaaaa gagattactg tcactttcac atatttctga gttaccgtat tctcatggtt
    19861 tctttttatt tataaggaat acccctgtgc gacaacggac tattgtatgc cctttgattc
    19921 aaaatgatgg tgcttatttg ctgtgtaaaa tggccgacga tcgcggcgtt ttccccggtc
    19981 aatgggcgct ttcgggtggc ggcgtggagc ctggcgaacg aattgaagag gcactacgcc
    20041 gcgaaattcg cgaagaactg ggagaacagc tgcttttgac agaaatcacg ccgtggacct
    20101 tcagcgatga tattcgcacc aagacgtatg cagatggtcg caaggaagag atttatatga
    20161 tttacctgat ttttgactgc gtttctgcca accgagatgt gaaaataaac gaagagtttc
    20221 aggactacgc gtgggtaaaa cctgaagatc tggtgcatta tgatttgaat gttgccaccc
    20281 gaaaaacgtt acgtttgaaa ggtcttcttt aacaatgatg acagccatca ctggctgtca
    20341 gattgaaaat ttagtggtta acgaattccc catccagata aactttgcct ttttccacat
    20401 gcatgactaa accatccaga taatcaggtt taaatgtcgc atcacgctta tctttagcaa
    20461 tatatgtcag gcaatgatta tgggtaaaaa taacgatatt tttatcaggt gctttgcgtt
    20521 gtaagtcttt aattgcacta taaatctcat taccgcattg aagaaatcgt ttatctaccg
    20581 tcagcttttt acccgccgaa aaccaggtcg ccgactgaat agtccggacg gtattactgg
    20641 aatagagatc aaaatcaggg atatcagcac taaaagcgtt gcccagttca cgggcatcct
    20701 gggtaccttt gaccgtaata cctgttttat ctgacaagca ttgattggtt gaacgatcgc
    20761 aacgttcagc atggcgaaac aaaacgacaa ccgggtgctg ctgtgccagt ctggccagtg
    20821 ttttattgtc gatgcgtggc aaaccattgc tactccaggc ggcatgagtc cccagtccgg
    20881 caattgcagc gagcgccagt aaaatgatga aatatttttt tgacttcaac gaagatcggc
    20941 aaaaagctaa cacgtaaact ccacctgtag acaagcgcaa ccagacaatt accgtgaaat
    21001 tgagcgactt ttctggcgat aattagcagt tggtgtaata ttaaaaatcc tatgatgccg
    21061 gcaaaatgcc tcaaaatttt gccaaatgca aagtctaaat aagaaaaaat ataaaaattt
    21121 caatatttac gtctaatatt agtttcttaa ggttaagtta atattctatc cttaaaattt
    21181 tgctccaaat ggcaaaatat acacaacact ctttatagca aatataagtg gacaggtatt
    21241 caatgacgga tggaaaaaca atgtcaggat ttttgccttt ttcgcgacca gcaatgggcg
    21301 tagaggaact cgctgcagtt aaagaggttc tcgaatccgg ttggatcaca accggtccga
    21361 agaatcaggc gcttgaacaa gctttttgcc agttgacggg aaatcagcat gccatcgcgg
    21421 tcagttcagc caccgccgga atgcatatca cgctaatggc gttggaaatt ggcaagggcg
    21481 atgaagtgat tacgccttcc ctgacctggg tttcaaccct caatatgatt tccttgctgg
    21541 gtgcaacgcc ggtaatggtg gatgtcgacc gcgatacgct gatggtcacg cctgaagcga
    21601 tcgaagcagc cattacacca cgcactaaag ccatcattcc ggtgcattat gccggtgcgc
    21661 cagcagatat tgacgccatt cgcgccattg gcgaacgtta cggcatcgca gttatcgaag
    21721 atgctgccca tgccgtaggt acgtattaca aagggcgaca tattggcgca aaaggtaccg
    21781 ctattttttc gtttcacgcc atcaaaaata ttacctgtgc ggaaggtggc ctgattgtta
    21841 ctgataatga aaatcttgcc cgccagctgc ggatgctgaa atttcacggt ctgggtgtcg
    21901 atgcctatga cagacatacc tggggccgtg caccgcaggc tgaagtctta acgccgggct
    21961 ataagtacaa tctgaccgat attaacgccg cgattgccct gacacagtta gtcaaattag
    22021 agcacctcaa cacccggcgg cgcgaaattg cccagcaata tcagcaagca ctggcaactc
    22081 tcccctttca gccattaagc cttcccgcct ggccgcacgt tcacgcctgg catctgttta
    22141 ttattcgtgt cgatgaacaa cgttgtggta tcagtcgcga tgcgttgatg gaagcgttaa
    22201 aagaaagagg cattggtacc gggttacatt tccgcgctgc tcacacacaa aaatattatc
    22261 gcgagcgttt tcccacgctg tcgttaccga ataccgaatg gaatagcgaa cgcatctgtt
    22321 cattgccgct gttcccggat atgaccaccg ccgatgccga ccgcgtcatc acagcccttc
    22381 agcaactcgc aggacaataa gccatgtttg aaatccaccc tgttaagaaa gtctcggtgg
    22441 ttattcccgt ttataacgag caggaaagct taccggaatt aatcaggcgc accaccgcag
    22501 cctgtgaatc actggggaaa gagtatgaaa tcctgctgat tgatgacggc agtagcgata
    22561 attccgcgca tgtgctggtc gaagcctcac aagcggaggg cagccatatt gtgtctattt
    22621 tgcttaaccg caattacggg caacattcag cgattatggc gggattcagt cacgtcaccg
    22681 gcgacttaat tattaccctc gatgccgatc tccagaatcc gccagaggag atcccccgtc
    22741 tggtggcaaa agccgatgaa ggttacgacg tggtagggac tgtacgccag aaccgccagg
    22801 acagctggtt tcgtaaaacc gcttcgaaga tgattaaccg gcttattcag cgcaccaccg
    22861 gcaaagcgat gggtgactat ggttgtatgc tgcgcgccta tcgccgtcat attgtcgatg
    22921 cgatgttgca ctgccatgaa cgcagcacct ttatcccgat tctggcgaat atcttcgccc
    22981 gccgtgccat tgaaattcca gtgcaccatg ccgagcgtga gtttggtgaa tccaaataca
    23041 gctttatgcg cctgattaat ttgatgtacg acctggtgac ctgccttacc acaacgccgc
    23101 tacgtatgtt gagtttgctc ggcagcatta ttgcgattgg cggttttagc attgcggtgc
    23161 tgctggtgat tttacgcctg accttcggac cacaatgggc ggcagaaggc gtctttatgc
    23221 tatttgccgt gctgtttact tttattggcg ctcagtttat cggcatggga ttgctcggtg
    23281 aatatatcgg caggatctac accgatgtcc gcgcccgccc ccgctatttt gttcagcaag
    23341 ttatccgtcc atccagcaag gaaaatgaat aatgaaaacc gtcgtttttg cctaccacga
    23401 tatgggatgc ctcggtattg aagccctgct gtctgccggt tacgaaatta gcgccatttt
    23461 tacccatact gataaccccg gtgaaaaagc cttttatggt tcggttgccc gactggcagc
    23521 ggaaagaggc attccagttt atgcgccgga taacgttaat catccgctgt gggtggaacg
    23581 cattgcccaa ctatcaccag aggtgatttt ctctttttat tatcgccatc ttatttgcga
    23641 tgagattttg cagctcgctc cgagaggtgc atttaatctg catggttcgc tgttaccaaa
    23701 atatcgtggt cgcgcgccgc tgaactgggt gctggtgaac ggtgaaacgg aaactggcgt
    23761 tacattgcac cgaatggtga aacgtgccga tgccggggcc attgtggcac aactgcgcgt
    23821 tgccattgcg ccagacgata tcgccattac gctgcatcat aaattgtgcc atgccgcgcg
    23881 ccagctactg gaacagacat tacccgccat taaacacggt aatattctgg aaatcgccca
    23941 gcgcgaaaac gaagccacct gttttggtcg cagaacgccg gatgacagtt tcctcgaatg
    24001 gcataagccg gcatccgtac tgcacaacat ggtgcgtgcc gttgccgatc cgtggccggg
    24061 tgccttcagc tatgttggca atcagaaatt caccgtctgg tcgtcgcgtg ttcatcctca
    24121 tgccagcaaa gcactgccgg ggagcgtgat ttctgttgca ccactgctga ttgcctgtgg
    24181 cgatggcgcg ctggaaatcg tcactggaca ggcgggcgac ggcattacta tgcagggctc
    24241 gcaattagcg cagacgctgg gcctggtgca aggttcacgc ttgaatagcc agcctgcctg
    24301 tgccgcccga cgccgtaccc gggtactcat cctcggggtg aatggcttta ttggcaacca
    24361 tctgacagaa cgcctgctgc gcgaagatca ttatgaagtt tacggtctgg atattggcag
    24421 cgatgcgata agccgttttc tgaatcatcc gcattttcac tttgtcgaag gcgatatcag
    24481 tattcattcc gaatggattg agtatcacgt caaaaaatgt gatgtcgtgt tgccgctggt
    24541 ggcgatagcc acgccgattg aatatacccg caacccgctg cgcgtatttg aactcgattt
    24601 cgaagagaat ctgcgcatta tccgctactg cgtgaagtac cgtaagcgaa tcatcttccc
    24661 gtcgacttca gaagtttatg ggatgtgtag cgataaatac ttcgatgagg atcattctaa
    24721 tttaatcgtc ggcccggtga ataaaccacg ctggatttat tcggtgtcta aacaattact
    24781 tgatcgggtg atctgggcct atggcgaaaa agaaggttta cagttcaccc tcttccgccc
    24841 gtttaactgg atggggccac gactggataa ccttaatgca gcgcgaattg gcagctcccg
    24901 cgctattacg caactcattc tcaatctggt agaaggttca ccgattaagc tgattgatgg
    24961 cggaaaacaa aaacgctgct ttactgatat tcgggatggt atcgaggcgt tataccgcat
    25021 tatcgaaaat gcgggaaatc gctgcgatgg cgaaattatc aacattggca atcctgagaa
    25081 cgaagcgagc attgaagaac tgggtgagat gctgctggcg agcttcgaaa aacatccgct
    25141 gcgccattac ttcccaccgt ttgcgggctt tcgtgttgtc gaaagtagca gctactatgg
    25201 caaaggatat caggacgtag agcatcgtaa accgagcatc cgcaatgccc gccgctgcct
    25261 ggactgggaa ccgaaaattg atatgcagga aaccatcgac gaaacgctgg atttcttcct
    25321 gcgcaccgtt gatcttacgg ataaaccatc atgaccaaag tcggcttacg cattgatgtc
    25381 gatacctttc gtggcacccg tgaaggcgtg ccgcgtctgc tggaaatctt gagtaagcat
    25441 aatattcagg ccagcatttt tttcagcgtc ggcccggaca atatgggccg ccatctctgg
    25501 cgactggtga agccacagtt tttgtggaag atgctgcgct caaacgcggc atcgctttat
    25561 ggctgggata ttttactggc aggtacggcc tggccaggta aagagattgg tcatgccaat
    25621 gccgatatca ttcgtgaagc ggctaaacat cacgaagtcg gcctgcacgc ctgggatcac
    25681 catgcctggc aagcccatag cggtaattgg gatcggcaaa caatgatcga cgatattgca
    25741 cgcggccttc gcaccctgga agagattatc ggtcaaccgg taacctgttc tgccgctgct
    25801 ggctggcgtg ctgaccaaca ggtgatcgaa gcaaaagaag cgttccattt gcgctacaac
    25861 agcgattgtc gtggggcgat gccgtttcgt ccattgctcg aatcaggaac ccccggcact
    25921 gcgcaaattc cggtgacctt acccacctgg gatgaagtga ttggtcggga tgtgaaagca
    25981 gaagatttta acggttggtt actcaaccgc atccagcgag ataaaggcac gccggtttat
    26041 accattcatg ccgaagttga aggctgcgct tatcagcata attttgtgga tctcctgaaa
    26101 cgcgcagctc aggaaggcgt gacattttgc cctttaagcg aactgttatc agggacgttg
    26161 ccgctcggac aagttgttcg cggaaatatt gccggacgtg aaggctggct gggttgccaa
    26221 caaatggcgg gtagtcactg atgaaatcgg tacgttacct tatcggcctc ttcgcattta
    26281 ttgcctgcta ttacctgtta ccgatcagca cgcgtctgct ctggcaacca gatgaaacgc
    26341 gttatgcgga aatcagtcgg gaaatgctgg catccggcga ctggattgtt ccccatctgt
    26401 tagggctacg ttatttcgaa aaacccattg ccggatactg gattaacagc attgggcaat
    26461 ggctatttgg cgcgaataac tttggtgtgc gggcaggcgt tatctttgcg accctgttaa
    26521 ctgccgcgct gatgacctgg tttactctgc gcttatggcg cgataaacgt ctggctttac
    26581 tcgccacagt aatttatctc tcattgttta ttgtctatgc catcggcact tatgccgtgc
    26641 tcgatccgtt tattgccttc tggctggtgg cgggaatgtg cagcttctgg ctggcaatgc
    26701 aggcacagac gtggaaaggc aaaagcgcag gatttttact gctgggaatc acctgcggca
    26761 tgggggtgat gaccaaaggt tttctcgccc ttgccgtgcc ggtattaagc gtgctgccat
    26821 gggtagcaac gcaaaaacgc tggaaagatc tctttattta cggctggctg gcggttatca
    26881 gttgcgtact gacggttctc ccctggggac tggcgatagc gcagcgggag cctgacttct
    26941 ggcactattt tttctgggtt gagcatattc aacgctttgc actggatgat gcccaacata
    27001 gagcgccgtt ctggtactac ttgccggtca tcattgccgg tagcctgccg tggctgggat
    27061 tactccccgg tgcactgtac gctggctgga aaaaccgcaa gcattccgca accgtttatt
    27121 tgttgagctg gacgataatg ccgctgctgt ttttctccgt cgctaaaggc aaattgccca
    27181 cctatattct ttcctgcttt gcacctctgg caatgctgat ggcgcattac gctttgctgg
    27241 cagcaaaaaa taatcctctg gcgttgcgga ttaatggctg gattaacatc gcttttggcg
    27301 tcactggcat tattgccaca tttgtggtct ccccctgggg accaatgaac acaccggtgt
    27361 ggcaaacctt cgagagctat aaagtctttt gtgcctggtc tattttttcg ctatgggcat
    27421 ttttcggctg gtacacctta acaaacgtcg aaaagacctg gccttttgcc gcgctttgcc
    27481 cgctggggct ggcgttgctg gtaggatttt caattcctga cagagtcatg gaaggaaaac
    27541 atccgcaatt ttttgtcgag atgacacaag aatcacttca gccaagccgc tatattctta
    27601 ctgatagcgt cggtgttgcc gcaggtctgg catggagcct gcaacgcgat gacatcatca
    27661 tgtatcgcca gacaggtgag ttgaaatatg gccttaatta tccggatgca aaagggagat
    27721 ttgtcagcgg tgatgagttc gcaaactggc ttaatcaaca tcgtcaggag gggattatta
    27781 cactcgtgct ttcggttgac cgcgatgaag atatcaatag tctcgccatt ccgcccgctg
    27841 atgccatcga tcgtcaggga cgtctggtac tcattcagta tcggcccaaa tgatctggct
    27901 aacattagtc tttgccagct tgcttagcgt tgccgggcag ttgtgtcaga aacaggcaac
    27961 ctgctttgcg gcggtaaaca aacggcgcaa acatatcgtg ctgtggctgg gactggcgct
    28021 ggcttgtctt ggccttgcca tggtgctctg gctgctggtc ttgcagaacg taccggtagg
    28081 cattgcctac ccgatgttaa gtctgaattt tgtctgggta acgctggctg cagtaaaact
    28141 gtggcacgag ccggtatcgc tgcgtcactg gtgtggggtg gcgttcatta ttggcggcat
    28201 tgtgatcctc gggagtacgg tgtaatgggc ctgatgtggg gattattcag cgtcattatc
    28261 gcctccgctg cgcaattaag cttgggtttt gcggcgagtc atctgccgcc aatgacgcac
    28321 ttatgggatt ttatcgcggc gctgttagct tttggcctgg atgcccggat attgttactc
    28381 gggctgctgg gttatctgct ttccgttttt tgttggtata aaacactaca taaacttgcg
    28441 ctaagcaaag cctatgcctt gctcagtatg agttatgtgc tggtatggat tgcttcaatg
    28501 gtacttcccg gctgggaagg gactttttcg ctgaaagcac tactgggagt agcctgtatt
    28561 atgagcgggt tgatgctgat tttcctgccc acaacaaaac aacgttactg agttttccct
    28621 gccgctttac actgccgttc ccactcatcg gggctataac agcttgcaga aagaactttc
    28681 accgtgtgca gcttttcacg attaatacag taaagcgcat tctgcaaagg cgagagtaaa
    28741 tcccccactt tcacggcgaa gtcactcttc acttcggcga tcatttttat cgcgccgcca
    28801 gcatcgcaga gcataagtac atgtctgtta ttttgtttat tgcagcacga ttttttgacc
    28861 agccattcca ttgcattatc ctgtttgata agagttttca catcaattta cagattaaca
    28921 gataaaccta ttatcaacgg caatggaatg gcaaaattgc gaagtgaaac taatgatttc
    28981 ttaatcctgg cgttgcaccc actcttttaa cgcctgacgt gaaattttaa taccgccgtt
    29041 tttcagctcc ggcggtaaag tcagccagcg caccggttgt tgaaaacggg ccagcttatc
    29101 tttcacccat tcgccaagat caacggtttg ctggtcatac tccaccaccg ctaccggtcg
    29161 atgaccaaac tccttgtcag caacgggaac gataaacacc tgcaaaaccg caggatgtgc
    29221 agcaataacg cgctcgactt cttctggctg aatgccttcg ccaccactga agaacagatt
    29281 gtctaaacgt ccgacaatgg tcagcttgcc attatgcatc tcaccgcgat cgcgcgtggc
    29341 gtaccagcct tcgtcattaa ccagtggaac ccgttgcccg ttacgccagt aaccttctgc
    29401 catgctggca gcccgcagcc acacttcgtc gtttacgatt ttcacttccc gacccggcag
    29461 cggcgaaccg acgtctgcca gaccgtcggc ttctttcgca cacaccgtgg aggcaaactc
    29521 ggtcagacca tagccgcaaa agcaccgaat cccctgctcg cgcgcctgtt ccgtcaactc
    29581 gaccgggata gccgcgccgc caagtaaaac cgctttcagg gaaacggaac tacggttaac
    29641 cagcaaacgc cagagttgtg ttggcaccag tgaagcgtgc gtacagcctg ccagcatttg
    29701 ctccaatggc tgtttatcac gtaccgtcat ccgcgcacca gcgtataacc agcgccacat
    29761 aattccctga ccggagacgt gaaacagcgg taaagagagc aaccagtcat cgtgatcgcc
    29821 aaacggaatc agcgataaca ccccttcggc gctggcaaga tgagcctgat aagtatgaac
    29881 agcggctttc ggcaagccag tggaaccaga ggttaacgtc attgagcaca gacgcgtcgg
    29941 ctgccacgcc acggcatgta cgccttcaac ctgctgaata tgcagcgacg ttaacgccgg
    30001 aaacgtgttt tccccttccg gcaccagcgc aaattgcagc gtaagattgg gcagcaattc
    30061 ctcaagcagc ggttgcggca gctgagggtt cacgggcaac acccgcgccc cgcattgcag
    30121 taacgccagc caggccagca gcgtttgcgg cgtattccac gcccgcaaca tcacgccgct
    30181 gcccgcaacc acaccctgcg ccgcaaagct ggaggctaat tcatcgacgc gagcacaaag
    30241 ctcgcgccag ttgagttgct cgtcattaag acgtaaggcg atggcttctc cccgcacttg
    30301 ccgccagtga cgccacggcc agtcagagaa gatcataaca gccgctccag tgcatcaacg
    30361 tccactaacg gcagtggact ccccggccag cgacgaacct gctgcacctg catcagatcc
    30421 agcgtgtcca gtcccggaat ggtgtctggc gttaaccagg cagcaatccg cgccagttgc
    30481 attagaccta agctcgactc aatggaagaa ctgatcaccg ccgtcagccc cagcgcatgc
    30541 gccgccttca cctgctcgcg tactttctcc aggctgcccg tgagcgtggg tttgataact
    30601 accgcgcgca cgccctcttc agctacaaag gcaaaatccg gctcgcgcaa gctttcatcc
    30661 caggcaatgg caatgccggt ttcacgggca aacgcacgcg aatcatcgcg ggttttgcac
    30721 ggctcttcga gaaatgcgat gcggtggcga taatccgggt taacgtattt ggcaaactgc
    30781 tggcctttca gcggtgtcca ggcgcgattc gcgtcaagac gcaaatgcag atccggaatt
    30841 gcctccagca acagattcac caccatgccg tcgcgcacgg cttcatacaa tcccactttg
    30901 accttcgcca ctttctcgcc aggcatatcg gcaagtttga ggatcagatc gtcaggatcg
    30961 ccattacaca gcggtgccgc acggtaattg gccgcctgcg ggagcgtatc tgccagctcc
    31021 gccaatgcac aacttacgcc aaaggcaacg gaaggcatct gcggtaattc gcaatcgcct
    31081 gccagccagt tatttaccca ggcaagcagc acactttgcg catcttccca ggtttcctga
    31141 ctgaagcccg gcagtgggga gatctccccc cacccttcgc gctcaccctc acgcaggcaa
    31201 acatacagcc cgtcgcgggt ttttaaccgc ctgtcgcgca gaaccacccc cgcgtccatg
    31261 gggatctgcc agcggtatac ctgcgcgcta cgcattacgg attccgttta aatttgctga
    31321 aatcaggctg acgtttctgg ttgaaagcat tgcgaccttc ctgaccttct tccgtcatgt
    31381 agaacagcat ggtagcgttg cccgccagct cctgcagccc cgcctgcccg tcacagtcgg
    31441 cgttcagcgc agctttcagg cagcgcagcg ccatcgggct gttttgcagc atttcacggc
    31501 accaacggac ggtttctttt tccagatccg ccagcggtac cacggtgttt acaaggccca
    31561 tatccagcgc ctgtttcgcg tcgtactgac ggcacaagaa ccagatttca cgcgcttttt
    31621 tctgtccgac gatgcgagcc atgtaggaag cgccccagcc gccgtcgaag gaaccgactt
    31681 tcgggccagt ctggccgaag atggcattat cagccgcgat agtcaggtcg cacatcatgt
    31741 gcagaacgtg accgccgccg atggagtagc cagccaccat cgcgacaacc ggtttcggac
    31801 aggtacggat ctgacgctgg aagtccagca cgttcaggtg atgtacgccg gaatcatctt
    31861 tatagccgcc gtaatcgcca cgcactttct ggtcaccacc ggagcagaac gctttatcgc
    31921 ctgcaccagt cagaatgatc acgccgatgt tgtcgtcata acgcgcatcc gccagcgcct
    31981 ggatcatctc ttttaccgtc agaggacgga aggcattgcg cacctgcgga cgattaatgg
    32041 tgatttttgc gataccgtcg gtggattttt cataacgaat gtcctcaaaa ccttcggagc
    32101 agtcgtgcca ttcaaccggt gcgtaaagca ttgcttcatc aggataaatc atattgtgtc
    32161 ctttagtcag aaacgcaaga tctgcgccag acttgcgatt acgccagcgg gattttcccg
    32221 gtgcgcgtta tgtccggcgc gaggaatgac atggcagtcg gcagccagtt ccgccgccag
    32281 agcgcggaat ttgctgtcac gttcaccaca taaataataa aacgcaaatg tgcgggcgct
    32341 aaggttagca cgtaaatcgg gctggacggc gagagaagtc gcctccagca tggcggcaag
    32401 ggttgcgcca ttattgttgc tgcgcagcgc caccagctcc cggcgttgat cgtcattgag
    32461 cgaggcaaaa acaggctgtt gataccaatc ggcaaatacc gccgttaacg gttctgtacg
    32521 aaaacgctgc gcccattggc gatcggaacg ctgacgttcc gcgcgttgtt cagcattttg
    32581 caacccagga tgcccgcctt cgacaacaac tccacaaagc cccgccagct cctggcaagc
    32641 cgccatcatc gccacccgtc cgccaagcga gtaccccacc agccagaagt taaggatgtt
    32701 gtaactaacc agcgttttac acagtaagtc ggtgacatca tcaaatccat cgacgctgat
    32761 cgtcgctgaa ccaccgtgac ctgggagatc aacatacaac cgcgagtagt cggcaaacgc
    32821 ctcgcccact tcttgccatt catggcaatc gccggaaaaa ccgtgcaaaa acaccagcca
    32881 gggtaaacct ggtttgccgt gttttgcctg cgcgtgcagg atcataaatg gcttacctgc
    32941 gccagaagtt gctggagcgt ttgcgcacca tcggtgtcgt taaccaccat ttcaatcacc
    33001 gtggtggtcg gcgtacgcca ggcatcgact agtgtcgttt caagttcctg ccagttttgc
    33061 ggacgatgat atttcagctc gaacatcgcg gcggcgtgct caaaatggac gttttgcggc
    33121 atcagataga aacgctcgcg ctcgcttttc ggcgttggca acagcgaaaa aatttgcccg
    33181 ccgttgttgt tcaccacaat caataccagc ggtgcggaaa cctggcgcaa taacgccagc
    33241 gcgttgagat cgtaaagtgc ggagagatcg cccacaatcg ccagcgtcgg tttgccgctt
    33301 gcccgctgaa cgccggcggc agtcgagagc agcccgtcga taccgctggc accacggttg
    33361 ctgtacaccg ggtaacctgc cggaagttgc gaaagcgcat caatcagacg caccaccagg
    33421 ctgttaccga caaacaattg cccctgttca ggcaaatagt cgctgatgcg atgcgccagt
    33481 tgcgcttcgc caaacgcatc gcgacgggca ataaccgcct gcattgcctg ttccgccagg
    33541 cgcgggattt caacgcacca gggctggcgt ttttctgccg gatgcagctc cagccagtcg
    33601 gcaatattgg caattaagcg acgtccgcga tggtgtgccg gatcaagtcg cccttcaatg
    33661 tcatcaacaa tccagtactc ttctggttca cagcttgcct gccattggag gagtcgtttc
    33721 cccgtcaggc tgcttcccaa ttgcaccact atttgtgcct gttgcagttc gctggtcgct
    33781 ttggcattgc ctaaccagag atcggcacac ggcagcggct gtccggtttg cgacagcacg
    33841 tcgccaatca gcggccagcc aagagtttgc gcccacagtg caactttttt gccctcttcc
    33901 gcactcatgc gcccggcaac caccacaccg cgcttttgtc gccagaagaa ccagtcgcgc
    33961 tgtttttcac tttcccgacg aggcgcttca cgcagccacg gtttgtcgtc ctgccaccag
    34021 tcgcccagcc gctgttgcca gctaatcccc gtgtcgtcca tttcgccata cagcggttca
    34081 gcaaacgggc agttgatatg gactccacca gcatgaagcg taccgagagc gtggtcgatg
    34141 gtagaaacca gccaacgtgc ggggatatcc tgggtcgggc gcggcagtga aatactgtgc
    34201 gtggggtgag aggcgaacat tcccggctga cgaatcgcct gattcgcgcc gcagtcaatc
    34261 agctccggcg ggcgatcggc ggttaacagg atcagttttt ctccggttaa cccagcttca
    34321 atcagcgccg gatagagatt tgctaccgcc gtgccggagg tcacaatcac cgccaccggc
    34381 tgcttgctga ctttcgccag ccccagcgcc agatgcccca gtccacgctc atcgaaatgg
    34441 gtgtggtgaa tgaatgcgga attctccgcc gccgctaacg ttaacggtgt agaacgcgag
    34501 ccaggggcga tacagatgtg tctgacgccg tgacgcgtta atgcttccag aatgaccgcc
    34561 gcccagcgtc ggttaaatgc gcttactgac atgagtttgt ccggtatcaa tattgcggct
    34621 aagtataagg agctacaaaa atagattatt gatatgaatc ggtaatgatg cgactcatta
    34681 ctattccatt tgtaataaag tacgcagccc tgccgctttg ttgtcgattt cctgccactc
    34741 ttgctcgggg tcggaaccac ggacaatgcc tgcgccagca tatagccgca cgatattgcc
    34801 gctgattttt gctgagcgca gggaaacgca gaattcactt tgctgtaatg aaagatagcc
    34861 cgcagaaccg gcgtaccatt ctcgagtgaa cggttcgtga cgggcgataa actgtcgcgc
    34921 cagatcgcgc ggtaagccag caactgctgc cgttggctgc aactgatgta aacagatcac
    34981 atcatccgct ttgttgagtg aagtccagat acagcggcga agatgctgta ctttacgtag
    35041 acgcagtacc tgcggcggta aaacatccag cgtccgggta tcggcctgca atcgttggca
    35101 gatatcttcc accaccaaca tgttctcgcg ctggttttta tcatccgcca tcagccactc
    35161 acctaactgc tgcgcctgct tatcatcagg atgatttgct accgttcccg ccagcgcttc
    35221 ggtacgcagc gctttgtcac gccgccgcca taaccgttcc ggtgaagaac caagaaaagc
    35281 attttcgcca tcaaaggcca tgtaaaaatg gtagcaattc agattgagcc gacgactggc
    35341 agccatcatc gctgccgcat tgaccggact tgcgaaatgc aggtcagttg cccgagcaag
    35401 cacaactttg tcgagctcgc cttcggcgat ggttttcgtt gccagttcga ttaatagcgt
    35461 ccagcccgtt ttgtccggcc agtgttgttc tcgcgtggtg gttaaatgta atccaggcag
    35521 gggcttgata ctcaccagtg tggcgataaa ttcttttgcc ttgattgcat cgtgctgaag
    35581 ggagctttcg ctgaatagcg tcaaccgcag cgtggcttta ccgccacaac gtcgccattc
    35641 caggcgtggt aaaagtaaat agccctgcga cgggtcaaac gcattcaacc cccagatgcg
    35701 taagtcggcg tgttccgggt gctggcgaag aaaacgttgt gcctggtcca acgacgtaaa
    35761 acgggtaatc gcacccagga cggcagcttc ttcatcacca ttacgttgtt gccagtagaa
    35821 ttgcggatac acctgctgac tggccagcca gctcaaggca tcaaaagcgt ctttgagagg
    35881 gaaaggaata tcgataaccc gaatgccggg tgtcgccgga atctcttgcg acaaatggcg
    35941 cagtagattt tccagcgccg tagtaagtga ttgcacgcgg acctctccct gttaaaagcc
    36001 tcacattata cggggtacga caaaaaaaat gcagtacccc ggtgtaggga ggttttaacg
    36061 gcgtgccagc aacagtccta gaaccagccc aacggccgca cccacaccaa ttccttgcca
    36121 gggtttttcg tggacgtagt catcagcacg ataaactgcc tgcttcgccc gatagtaata
    36181 actgtctgaa gcctggctaa cccgtttttt cacatcatcc agcgcttttt ctgcacgcgc
    36241 tttcagctca acatatttct gatcggcggg atcgccagag gagcggagca cctcttccag
    36301 tgtttcacta agcagcgtca ggtcgtcatc aatacgtgta tcaccaaact gattagacat
    36361 tctcgttctc catttgtgta aaacctgtca ctaactatag acaagggtta ttgattacgc
    36421 ctgaattacc tcgcgtgcca tcccaatgtg tgggatacca tcttcttcat aaatatcggt
    36481 cacagggatg aaaccaaatt gcccataaaa gttttgtaaa tgcgcctgcg cccccagata
    36541 aatcggcttc tccggccagc ggcgagtaca cgattccagc gccttactca tcaattgctg
    36601 accgattttt tcaccgcgta aggcttcgct gacaatcacc cgaccaatca caacgggttg
    36661 cagttcgtcg tcacttttca gaatcctcgc atacgccacc agtgtcccgt tatgccaacc
    36721 gagaatgtgg cgattttctc cctccagatc gtcaccatcg atatcctggt aaggacagtt
    36781 ttgttccact acaaataccg cacaacgcag ttgtaataag gcataaagct gagaaacgga
    36841 taaatcagaa tgatgcagat cttgccagtc gatcatggaa ggctcctttg tgctgtttgc
    36901 tttatactag ccggaatatt ttcagcagcg tgatgaactc atggaattaa tttttttagg
    36961 tacttcagcc ggtgtgccaa cccgtacgcg taatgtcacg gcaatattgc ttaacctgca
    37021 acatccgacc cagagcggac tttggttatt tgactgcggt gaaggcaccc agcatcagct
    37081 actgcatact gcctttaacc ctggaaaact ggacaagatt tttatcagtc accttcatgg
    37141 cgatcatctc tttggtttac ccggcttgct gtgcagtcgt tctatgtcag gcattatcca
    37201 acccttaacg atttatggtc cgcacggtat ccgtgaattt gtggagaccg cgctgcggat
    37261 tagcggctca tggaccgatt atccgctgga aattgtcgaa attagcactg gcgaaatttt
    37321 cgatgatggc ctgcgcaaag tgaccgctta tccgatggaa cacccactgg aatgttatgg
    37381 ttatcgtatt gaagaacatg ataaaccagg tgcattaaat gcccaggcat taaaagctgc
    37441 tggcgtgccg cctggcccgc tgtttcagga attaaaagcg ggcaaaacaa tcatgctgga
    37501 tgatggaagg cagattaatg gcgcagatta cttagccgct ccagtgccgg gtaaagcgct
    37561 cgctattttc ggcgataccg gcccctgcga tgccgcgctg gagcttgcta aaggcgtcga
    37621 cgtaatggtt cacgaagcga ctctggatat ggcgatggag gccaaagcca acagtcgcgg
    37681 ccatagttct acacgccagg cggcggcact agcccgtgag gctggagtcg gtaagctaat
    37741 cattacccac gtcagctcac gctatgatga caaaggttgt cagcacctgt tacgtgaatg
    37801 tcggtcaatt ttcccggcga ctgaactggc gaatgatttc gcggtgttta gcatttaagt
    37861 cgaaagcggt ggttagttag cagattaatt taacgatacc aacacattta tttgacattt
    37921 tttcatctgt ttgccggata cgtttgatgt cgatatcagg caaacagaat tatcattttt
    37981 agttttgtga attgtccaga tctttcgcca gtccaatcag gcgaataaat tccgctctgt
    38041 aaccctgtgg atcttcccct tttgccttct gagcccactg tttgatctgc tgccaggagg
    38101 tattgttcag gtattcagaa ccgcgtaact tttgcccata tgcagccact gctgcgcgaa
    38161 agcgcatatc ttctgaaggt gcctttattg cagatgccaa cgggaattca actaactgac
    38221 tttcttttcc ctgtggggat ttccaacgaa tctttaacca ggcaagttct tttgttttgt
    38281 ccgatttcgc tgatttgtta tccggggcat agcgtaactt atcaattgat gctttttgcc
    38341 ctttcagcgt taattcaaat aacaatgtta tatgtttacc tgcgccaata tcacccgcat
    38401 caacgttgtc gttattaaaa tcctctgcct taagttggcg cttttcataa cctatctgac
    38461 ggtattccgt tacccacgcg ggattaaact caatttgcgc tttgacatct tttgctacgg
    38521 taatcaacgt ctgccgcatt tcactgttca acactttctg cgcctctgag agggtatcaa
    38581 tgtagctata gttgccgtta ccaacatcgg caattctcac catcattgcc tcgttataat
    38641 tgctatcccc cacgccaagc gtcgacagag taacaccgga ctcacgctgt tttttgacca
    38701 ttgattcaat cgattttgga tcgtcaatgc caacgttaaa gtcaccgtca gtggccaata
    38761 aaatgcgatt gataccacct ttaataaacc ccttcgctgc ctgctgataa gccatctcca
    38821 gcccggcacc gccattggta ctgccttctg catccagcga atcaattgca gcattaattt
    38881 ccgccttgtg actaccggag atagaaggca acgcaatacg agagtcgcca gcgtaggtca
    38941 cgatggcaat gttatcctgc tcacgaagtt ctttgaccaa taatttcaac gaagactgga
    39001 taagtggcaa acgttcatca gaaatcattg aaccagacgt gtcgataaga aagaccagat
    39061 tagaagcggg taactcttca cttttgcgat ctttcgccag gatatcaact ttcagcaatg
    39121 ttcgctgttc attccagggt gcaggtgcca attcgtagcg catagcgaaa ggtattggct
    39181 tagaggccgg aatagattgt ttgtctttga tatcccagtc agacgggaaa taattgacta
    39241 tctcctccac ccgcacagcg tctggcggag gtaacagccc ttgattaagg aaacgccgca
    39301 cattcgcata actgccggta tcgacgtcaa ggctaaacgt cgccaacggg ttttgcgcta
    39361 cctgcttaac cggattatca tcgaactgct ggtagcgagc ggttgctgga tttgcgattt
    39421 gggaggctgc tttttctctg gctgcatgtt gatattttgg agccgcctgc aatcgcccca
    39481 gcaaagcctg tttgtctgaa tattgttgca cttcttgctg ggctaaggct ttcgcgtctg
    39541 cagcggcttt ggcggcgctt tgctcagcca cttttgttgc cgcttgttgc gcggcaagca
    39601 cttgctggtc agtgggtgta gtagattgtt gttgctgact tttcttatcc tcaggttccg
    39661 gcccacatcc tgacaaaatc aaactactta taagcaacat gattata
//
LOCUS       DAHHTG010000030        39480 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.030, whole genome shotgun sequence.
ACCESSION   DAHHTG010000030 DAHHTG010000000
VERSION     DAHHTG010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 39480)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39480)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39480
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.030"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            14..577
                     /gene="srlA"
                     /locus_tag="NBI74_001553"
                     /gene_synonym="gutA"
     CDS             14..577
                     /gene="srlA"
                     /locus_tag="NBI74_001553"
                     /gene_synonym="gutA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HCD1931912.1"
                     /translation="MIETITHGAEWFIGLFQKGGEVFTGMVTGILPLLISLLVIMNAL
                     INFIGQHRIERFAQRCAGNPVSRYLLLPCIGTFVFCNPMTLSLGRFMPEKYKPSYYAA
                     ASYSCHSMNGLFPHINPGELFVYLGIASGLTTLNLPLGPLAVSYLLVGLVTNFFRGWV
                     TDLTTAIFEKKMGIQLEQKVRLAGATS"
     gene            574..1533
                     /gene="srlE"
                     /locus_tag="NBI74_001554"
                     /gene_synonym="gutE"
     CDS             574..1533
                     /gene="srlE"
                     /locus_tag="NBI74_001554"
                     /gene_synonym="gutE"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HCD1931913.1"
                     /translation="MTRIRIEKGTGGWGGPLELEATPGKKIVYITAGTRPAIVDKLAQ
                     LTGWQAIDGFKEGEPAEAEIGVAVIDCGGTLRCGIYPKRRIPTINIHSTGKSGPLAQY
                     IVEDIYVSGVKEENITVVGEATPQPSSVGRDYDTSKKITEQSDGLLAKVGMGMGSAVA
                     VLFQSGRDTIDTVLKTILPFMAFVSALIGIIMASGLGDWIAHGLAPLASHPLGLVMLA
                     LICSFPLLSPFLGPGAVIAQVIGVLIGVQIGLGNIPPHLALPALFAINAQAACDFIPV
                     GLSLAEARQDTVRVGVPSVLVSRFLTGAPTVLIAWFVSGFIYQ"
     gene            1544..1915
                     /gene="srlB"
                     /locus_tag="NBI74_001555"
                     /gene_synonym="gutB"
     CDS             1544..1915
                     /gene="srlB"
                     /locus_tag="NBI74_001555"
                     /gene_synonym="gutB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HCD1931914.1"
                     /translation="MTVIYQTTITRIGASATDALSDQMLITFREGAPADLEEYCFIHC
                     HGELKGALHPGLQFTLGRHRYPVTAVGSVAEDNLRELGHVTLRFDGLSEAEFPGTVHV
                     AGPVPDDIAPGSVLKFESVKE"
     gene            1919..2698
                     /gene="srlD"
                     /locus_tag="NBI74_001556"
     CDS             1919..2698
                     /gene="srlD"
                     /locus_tag="NBI74_001556"
                     /EC_number="1.1.1.140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sorbitol-6-phosphate dehydrogenase"
                     /protein_id="HCD1931915.1"
                     /translation="MNQVAVVIGGGQTLGAFLCHGLAAEGYRVAVVDIQSDKAANVAQ
                     EINAEYGEGTAYGFGADATSEQSVLALSRGVDEIFGRVDLLVYSAGIAKAAFISDFQL
                     GDFDRSLQVNLVGYFLCAREFSRLMIRDGIQGRIIQINSKSGKVGSKHNSGYSAAKFG
                     GVGLTQSLALDLAEYGITVHSLMLGNLLKSPMFQSLLPQYATKLGIKPEQVEQYYIDK
                     VPLKRGCDYQDVLNMLLFYASPKASYCTGQSINVTGGQVMF"
     gene            2804..3163
                     /gene="gutM"
                     /locus_tag="NBI74_001557"
     CDS             2804..3163
                     /gene="gutM"
                     /locus_tag="NBI74_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
                     /protein_id="HCD1931916.1"
                     /translation="MVSALITVAVIAWCAQLALGGWQISRFNRAFDTLCQQGRVGVGR
                     SSGRFKPRVVVAIALDDQQRIVDTLFMKGLTVFARPQKIPAITGMHAGDLQPDVIFPH
                     DPLSQNALSLALKLKRG"
     gene            3230..4003
                     /gene="srlR"
                     /locus_tag="NBI74_001558"
                     /gene_synonym="gutR"
     CDS             3230..4003
                     /gene="srlR"
                     /locus_tag="NBI74_001558"
                     /gene_synonym="gutR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucitol operon DNA-binding transcriptional
                     repressor SrlR"
                     /protein_id="HCD1931917.1"
                     /translation="MKPRQRQAAILEYLQKQGKCSVEELAQYFDTTGTTIRKDLVILE
                     HAGTVIRTYGGVVLNKEESDPPIDHKTLINTHKKELIAEAAVGFIHDGDSIILDAGST
                     VLQMVPLLSRFNNITVMTNSLHIVNALSELDNEQTILMPGGTFRKKSASFHGQLAENA
                     FEHFTFDKLFMGTDGIDLNAGVTTFNEVYTVSKAMCNAAREVILMADSSKFGRKSPNV
                     VCSLESVDKLITDAGIDPAFRQALEEKGIDVIITGESNE"
     gene            3996..4961
                     /gene="gutQ"
                     /locus_tag="NBI74_001559"
     CDS             3996..4961
                     /gene="gutQ"
                     /locus_tag="NBI74_001559"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase GutQ"
                     /protein_id="HCD1931918.1"
                     /translation="MSEALLNAGRQTLMLELQEASHLPERLGDDFVRAANIILHCEGK
                     VVVSGIGKSGHIGKKIAATLASTGTPAFFVHPAEALHGDLGMIESRDVMLFISYSGGA
                     KELDLIIPRLEDKSIALLAMTGKPTSPLGLAAKAVLDISVEREACPMHLAPTSSTVNT
                     LMMGDALAMAVMQARGFNEEDFARSHPAGALGARLLNKVHHLMRRDDAIPQVALTASV
                     MDAMLELSRTGLGLVAVCDDQRLVKGVFTDGDLRRWLVGGGALTTPVNEAMTVDGTTL
                     QSQSRAIDAKEILMKRKITAAPVVDENGKLTGAINLQDFYQAGII"
     gene            complement(4958..6472)
                     /gene="norR"
                     /locus_tag="NBI74_001560"
     CDS             complement(4958..6472)
                     /gene="norR"
                     /locus_tag="NBI74_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide reductase transcriptional regulator
                     NorR"
                     /protein_id="HCD1931919.1"
                     /translation="MSFSVDVLANIAIELQRGIGHQDRFQRLITTLRQVLECDASALL
                     RYDSRQFIPLAIDGLAKDVLGRRFALEGHPRLEAIARAGDVVRFPADSELPDPYDGLI
                     PGQESLKVHACVGLPLFAGQNLIGALTLDGMQPDQFDVFSDEELRLIAALAAGALSNA
                     LLIEQLESQNMLPGDAAPFEAVKQTQMIGLSPGMTQLKKEIEIVAASDLNVLISGETG
                     TGKELVAKAIHEASPRAVNPLVYLNCAALPESVAESELFGHVKGAFTGAISNRSGKFE
                     MADNGTLFLDEIGELSLALQAKLLRVLQYGDIQRVGDDRSLRVDVRVLAATNRDLREE
                     VLAGRFRADLFHRLSVFPLSVPPLRERGDDVILLAGYFCEQCRLRLGLSRVVLSAGAR
                     NLLQHYNFPGNVRELEHAIHRAVVLSRATRSGDEVILEAQHFAFPEVTLPPPEAAAVP
                     VVKQNLREATEAFQRETIRQALAQNHHNWAACARMLETDVANLHRLAKRLGLKD"
     gene            6659..8098
                     /gene="norV"
                     /locus_tag="NBI74_001561"
     CDS             6659..8098
                     /gene="norV"
                     /locus_tag="NBI74_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic nitric oxide reductase
                     flavorubredoxin"
                     /protein_id="HCD1931920.1"
                     /translation="MSIVVKNNIHWVGQRDWEVRDFHGTEYKTLRGSSYNSYLIREEK
                     NVLIDTVDHKFSREFVQNLRNEIDLADIDYIVINHAEEDHAGALTELMAQIPDTPIYC
                     TANAIDSINGHHHHPEWNFNVVKTGDTLDIGNGKQLIFVETPMLHWPDSMMTYLTGDA
                     VLFSNDAFGQHYCDEHLFNDEVDQTELFEQCQRYYANILTPFSRLVTPKITEILGFNL
                     PVDMIATSHGVVWRDNPTQIVELYLKWAADYQEDRITIFYDTMSNNTRMMADAIAQGI
                     AETDPRVAVKIFNVARSDKNEILTNVFRSKGVLVGTSTMNNVMMPKIAGLVEEMTGLR
                     FRNKRASAFGSHGWSGGAVDRLSTRLQDAGFEMSLSLKAKWRPDQDALELCREHGREI
                     ARQWALAPLPQSTVNTVVKEETSAATTADLGPRMQCSVCQWIYDPAKGEPMQDVAPGT
                     PWSEVPDNFLCPECSLGKDVFDELASEAK"
     gene            8095..9228
                     /gene="norW"
                     /locus_tag="NBI74_001562"
     CDS             8095..9228
                     /gene="norW"
                     /locus_tag="NBI74_001562"
                     /EC_number="1.18.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavorubredoxin reductase NorW"
                     /protein_id="HCD1931921.1"
                     /translation="MSNGIVIIGSGFAARQLVKNIRKQDACIPLTLIAADSMDEYNKP
                     DLSHVISQGQRADDLTRQTAGEFAEQFNLRLFPHTWVTDIDAEAHVVKSQNNQWQYDK
                     LVLATGASAFVPPVPGRELMLTLNSQQEYRACETQLRDARRVLIVGGGLIGSELAMDF
                     CRAGKAVTLIDNAASILASLMPPEVSSRLQHRLTEMGVHLLLKSQLQGLEKTDSGILA
                     TLDRQRCIEVDAVIAATGLRPETALARRAGLTINRGVCVDSYLQTSNADIYALGDCAE
                     INGQVLPFLQPIQLSAMVLAKNLLGNNTPLKLPAMLVKIKTPELPLHLAGEPQRQDLR
                     WQINTERQGMVARGVDDADQLRAFVVSEDRMKEAFGLLKTLSM"
     gene            complement(9450..11702)
                     /gene="hypF"
                     /locus_tag="NBI74_001563"
     CDS             complement(9450..11702)
                     /gene="hypF"
                     /locus_tag="NBI74_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HCD1931922.1"
                     /translation="MAKNTSCGVQLRIRGKVQGVGFRPFVWQLAQQLNLHGDVCNDGD
                     GVEVRLLEEPETFLVQLHQHCPPLARIDSVEREPFIWSQLPTEFTIRQSTGGAMNTQI
                     VPDAATCPACLAEMNTPGERRYRYPFINCTHCGPRFTIIRAMPYDRPFTVMAAFPLCP
                     ACDKEYCDPLDRRFHAQPVACPECGPHLEWVSHGEHAEQEAALQAAIAQLKMGNIVAI
                     KGIGGFHLACDARNSNAVATLRARKHRPAKPLAVMLPVADGLPDAARQLLTTPAAPIV
                     LVDKKYVPELCDDIAPGLNEVGVMLPANPLQHLLLQELQCPLVMTSGNLSGKPPAISN
                     EQALADLQGIADGFLIHNRDIVQRMDDSVVRESGEMLRRSRGYVPDALALPPGFKNVP
                     PVLCLGADLKNTFCLVRGEQAVLSQHLGDLSDDGIQMQWREALRLMQNIYDFTPQYVV
                     HDAHPGYVSSQWAREMNLPTQTVLHHHAHAAACLAEHHWPLDGGDVIALTLDGIGMGE
                     NGALWGGECLRVNYRECEHLGGLPAVALAGGDLAAKQPWRNLLTQCLRFVPEWQSYPE
                     TVSVQQQNWSVLARAIERGINAPLASSCGRLFDAVAAALGCAPATLSYEGEAACALEA
                     LATSCHGVTHPVTMPLVDNQLDLATFWQQWLNWQAPVNQRAWAFHDALAQGGAALMRE
                     QATMRGITTLVFSGGVIHNRLLRARLAHYLADFTLLFPQSLPAGDGGLSLGQGVIAAA
                     RWLAGEVQNG"
     gene            complement(11855..12382)
                     /gene="hydN"
                     /locus_tag="NBI74_001564"
     CDS             complement(11855..12382)
                     /gene="hydN"
                     /locus_tag="NBI74_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport protein HydN"
                     /protein_id="HCD1931923.1"
                     /translation="MNRFIIADASKCIGCRTCEVACVVSHQENQDCASLTPETFLPRI
                     HVIKGVNISTATVCRQCEDAPCANVCPNGAISRDKGFVHVMQERCIGCKTCVVACPYG
                     AMEVVVRPVIRNSGAGLNVRADKAEANKCDLCNHREDGPACMAACPTHALICVDRNKL
                     EQLSAEKRRRTALMF"
     gene            12810..13247
                     /locus_tag="NBI74_001565"
     CDS             12810..13247
                     /locus_tag="NBI74_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000536064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1931924.1"
                     /translation="MGSWGMEALESDEGLDLINWVEEQLQDDSTFDAESIVQRLSQHE
                     DLFGFQGDEEFLYDNYDNNVIGLVELIIQKAAGKKITSSKQIDQLDGYQLTSTFSKKL
                     QGRLQTIDDTHEWIMLFEGRAREKAKAYLIELTDKLRVVKTTA"
     gene            complement(13339..13758)
                     /locus_tag="NBI74_001566"
     CDS             complement(13339..13758)
                     /locus_tag="NBI74_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001696729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCD1931925.1"
                     /translation="MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEE
                     DDTNPLIDFLASRIAEYENNNEKFAEFDKAVAAMPVGVALLRTLIDQHNLTYADLKNE
                     IGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL"
     gene            complement(13784..13969)
                     /locus_tag="NBI74_001567"
     CDS             complement(13784..13969)
                     /locus_tag="NBI74_001567"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1931926.1"
                     /translation="MNIKTGMCAFLLSLVLPAQATSFTEYLPMSDSEYAQKRALKPLL
                     TMPYDAEQTWHFRKVGV"
     gene            complement(13994..14461)
                     /gene="hycI"
                     /locus_tag="NBI74_001568"
     CDS             complement(13994..14461)
                     /gene="hycI"
                     /locus_tag="NBI74_001568"
                     /EC_number="3.4.23.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation peptidase HycI"
                     /protein_id="HCD1931927.1"
                     /translation="MTDVLLCVGNSMMGDDGAGPLLAEKCAAAPKGNWVVIDGGSAPE
                     NDIVAIRELRPTRLLIVDATDMGLNPGEIRIIDPADIAEMFMMTTHNMPLNYLIDQLK
                     EDIGEVIFLGIQPDIVGFYYPMTQPIKDAVETVYQRLEGWEGNGGFAQLAVEE"
     gene            complement(14454..14864)
                     /gene="hycH"
                     /locus_tag="NBI74_001569"
     CDS             complement(14454..14864)
                     /gene="hycH"
                     /locus_tag="NBI74_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase assembly protein HycH"
                     /protein_id="HCD1931928.1"
                     /translation="MSEKVVFSQLSRKFIDENDATPAEAQQVVYYSLAIGHHLGVIDC
                     LEAALTCPWDDYLAWIATLEAGSEARRKMEGVPKYGEIVIDINHVPMLANAFDKARAA
                     QTSQQKEWSTMLLSMLHDIHQENAIYLMVRRLRD"
     gene            complement(14861..15628)
                     /gene="hycG"
                     /locus_tag="NBI74_001570"
     CDS             complement(14861..15628)
                     /gene="hycG"
                     /locus_tag="NBI74_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycG"
                     /protein_id="HCD1931929.1"
                     /translation="MSNLLGPRDANGIPVPMTVDESIASMKASLLKKIKRSAYVYRVD
                     CGGCNGCEIEIFATLSPLFDAERFGIKVVPSPRHADILLFTGAVTRAMRSPALRAWQS
                     APDPKICISYGACGNSGGIFHDLYCVWGGTDKIVPVDVYIPGCPPTPAATLYGFAMAL
                     GLLEQKIHARGPGEQDEQPADILHPDMVQPLRVKVDREARRLAGYRYGRQIADDFLTQ
                     LGQGEEQVARWLEAENDPRLNEIVSHLNHVVEEARIR"
     gene            complement(15628..16170)
                     /gene="hycF"
                     /locus_tag="NBI74_001571"
     CDS             complement(15628..16170)
                     /gene="hycF"
                     /locus_tag="NBI74_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycF"
                     /protein_id="HCD1931930.1"
                     /translation="MFTFIKKVIKTGTATSSYPLEPIAVDKNFRGKPEQNPQQCIGCA
                     ACVNACPSNALTVETDLATGELAWQFNLGRCIFCGRCEEVCPTAAIKLSQEYELAVWK
                     KEDFLQQSRFALCNCRVCNRPFAVQKEIDYAIALLKHNGDSRAENHRESFETCPECKR
                     QKCLVPSDRIELTRHMKEAI"
     gene            complement(16180..17889)
                     /gene="hycE"
                     /locus_tag="NBI74_001572"
     CDS             complement(16180..17889)
                     /gene="hycE"
                     /locus_tag="NBI74_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycE"
                     /protein_id="HCD1931931.1"
                     /translation="MSEEKLGQHYLAALNEAFPGVVLDHAWQTKDQLTVTVKVNYLPE
                     VVEFLYYKQGGWLSVLFGNDERKLNGHYAVYYVLSMEKGTKCWVTVRVEVDANKPEYP
                     SVTPRVPAAVWGEREVRDMYGLIPVGLPDERRLVLPDDWPDELYPLRKDSMDYRQRPA
                     PTTDAETYEFINELGDKKNNVVPIGPLHVTSDEPGHFRLFVDGENIIDADYRLFYVHR
                     GMEKLAETRMGYNEVTFLSDRVCGICGFAHSTAYTTSVENAMGIQVPERAQMIRAILL
                     EVERLHSHLLNLGLACHFTGFDSGFMQFFRVRETSMKMAEILTGARKTYGLNLIGGIR
                     RDLLKDDMIQTRQLAQQMRREVQELVDVLLSTPNMEQRTVGIGRLDPEIARDFSNVGP
                     MVRASGHARDTRADHPFVGYGLLPMEVHSEQGCDVISRLKVRINEVYTALNMIDYGLD
                     NLPGGPLMVEGFTYIPHRFALGFAEAPRGDDIHWSMTGDNQKLYRWRCRAATYANWPT
                     LRYMLRGNTVSDAPLIIGSLDPCYSCTDRMTVVDVRKKKSKVVPYKELERYSIERKNS
                     PLK"
     gene            complement(17907..18830)
                     /gene="hycD"
                     /locus_tag="NBI74_001573"
     CDS             complement(17907..18830)
                     /gene="hycD"
                     /locus_tag="NBI74_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycD"
                     /protein_id="HCD1931932.1"
                     /translation="MSVLYPLIQALVLFAVAPLLSGITRVARARLHNRRGPGVLQEYR
                     DIIKLLGRQSVGPDASGWVFRLTPYVMVGVMLTIATALPVVTVGSPLPQLGDLITLLY
                     LFAIARFFFAISGLDTGSPFTAIGASREAMLGVLVEPMLLLGLWVAAQVAGSTNISNI
                     TDTVYHWPLSQSIPLVLALCACAFATFIEMGKLPFDLAEAEQELQEGPLSEYSGSGFG
                     VMKWGISLKQLVVLQMFVGVFIPWGQMETFTVGGLLLALVIAIVKLVVGVLVIALFEN
                     SMARLRLDITPRITWAGFGFAFLAFVSLLAA"
     gene            complement(18833..20659)
                     /gene="hycC"
                     /locus_tag="NBI74_001574"
     CDS             complement(18833..20659)
                     /gene="hycC"
                     /locus_tag="NBI74_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit 3"
                     /protein_id="HCD1931933.1"
                     /translation="MSAISLINSGVAWFVAAAVLAFLFSFQKALSGWIAGIGGAVGSL
                     YTAAAGFTVLTGAVGVSGALSLVSYDVQISPLNAIWLITLGLCGLFVSLYNIDWHRHA
                     QVKCNGLQINMLMAAAVCAVIASNLGMFVVMAEIMALCAVFLTSNSKEGKLWFALGRL
                     GTLLLAIACWLLWQRYGTLDLRLLDMRMQQLPLGSDIWLLGVIGFGLLAGIIPLHGWV
                     PQAHANASAPAAALFSTVVMKIGLLGILTLSLLGGNAPLWWGIALLVLGMITAFVGGL
                     YALMEHNIQRLLAYHTLENIGIILLGLGAGVTGIALEQPALIALGLVGGLYHLLNHSL
                     FKSVLFLGAGSVWFRTGHRDIEKLGGIGKKMPVISIAMLVGLMAMAALPPLNGFAGEW
                     VIYQSFFKLSNSGAFVARLLGPLLAVGLAITGALAVMCMAKVYGVTFLGAPRTKEAEN
                     ATCSPLLMSVSVVALAICCVIGGVAAPWLLPMLSAAVPLPLEPANTTVSQPMITLLLI
                     ACPLLPFIIMAICKGDRLPSRSRGAAWVCGYDHEKSMVITAHGFAMPVKQAFAPVLKL
                     RKWLNPVSLVPGWQCEGSALLFRRMALVELAVLVVIIVSRGA"
     gene            complement(20656..21267)
                     /gene="hycB"
                     /locus_tag="NBI74_001575"
     CDS             complement(20656..21267)
                     /gene="hycB"
                     /locus_tag="NBI74_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycB"
                     /protein_id="HCD1931934.1"
                     /translation="MNRFVIADSTLCIGCHTCEAACSETHRQHGLQSMPRLRVMLNEK
                     ESAPQLCHHCEDAPCATVCPVNAITRVDGAVQLNESLCVSCKLCGIACPFGAIEFSGS
                     RPLDIPANTNTPKAPPAPPAPARVSTLLDWVPGIRAIAVKCDLCSFDEQGPACVRMCP
                     TKALHLVDNTDIARVSKRKRELTFNTDFGDLTLFQQAQSGEAK"
     gene            complement(21392..21853)
                     /gene="hycA"
                     /locus_tag="NBI74_001576"
     CDS             complement(21392..21853)
                     /gene="hycA"
                     /locus_tag="NBI74_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase regulator HycA"
                     /protein_id="HCD1931935.1"
                     /translation="MTIWEISEKADYIAQRHRRLQDQWHIYCNSLVQGITLSKARLHH
                     AMSCAPDKELCFVLFEHFRIYVTLADGFNSHTIEYYVETKDGEDKQRIAQAQLSIDGM
                     IDGKVNIRDREQVLEHYLEKIAGVYDSLYTAIENNVPVNLSQLVKGQSPVA"
     gene            22065..22415
                     /gene="hypA"
                     /locus_tag="NBI74_001577"
     CDS             22065..22415
                     /gene="hypA"
                     /locus_tag="NBI74_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HCD1931936.1"
                     /translation="MHEITLCQRALELIEQQAAKHGAKRVTGVWLKIGAFSCVETSSL
                     AFCFDLVCRGSVAEGCKLHLEEQEAECWCETCQQYVTLLTQRVRRCPQCHGDMLQIVA
                     DDGLQIRRIEIDQE"
     gene            22419..23291
                     /gene="hypB"
                     /locus_tag="NBI74_001578"
     CDS             22419..23291
                     /gene="hypB"
                     /locus_tag="NBI74_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HCD1931937.1"
                     /translation="MCTTCGCGEGNLYIEGDEHNPHSAFRSAPFAPAARPKMKITGIK
                     APEFTPSQTEEGDLHYGHGEAGTHAPGMSQRRMLEVEIDVLDKNNRLAERNRARFAAR
                     KQLVLNLVSSPGSGKTTLLTETLMRLKDSVPCAVIEGDQQTVNDAARIRATGTPAIQV
                     NTGKGCHLDAQMIADAAPRLPLDDNGILFIENVGNLVCPASFDLGEKHKVAVLSVTEG
                     EDKPLKYPHMFAAASLMLLNKVDLLPYLNFDVEKCIACAREVNPEIEIILISATSGEG
                     MDQWLNWLETQRCA"
     gene            23282..23554
                     /gene="hypC"
                     /locus_tag="NBI74_001579"
     CDS             23282..23554
                     /gene="hypC"
                     /locus_tag="NBI74_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 3 maturation protein HypC"
                     /protein_id="HCD1931938.1"
                     /translation="MCIGVPGQIRTIDGNQAKVDVCGIQRDVDLTLVGSCDENGQPRV
                     GQWVLVHVGFAMSVINEAEARDTLDALQNMFDVEPDVGALLYGEEK"
     gene            23554..24675
                     /gene="hypD"
                     /locus_tag="NBI74_001580"
     CDS             23554..24675
                     /gene="hypD"
                     /locus_tag="NBI74_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HCD1931939.1"
                     /translation="MRFVDEYRAPEQVMQLIEHLRERASHLSYTAERPLRIMEVCGGH
                     THAIFKFGLDQLLPENVEFIHGPGCPVCVLPMGRIDTCVEIASHPEVIFCTFGDAMRV
                     PGKQGSLLQAKARGADVRIVYSPMDALKLAQENPTRKVVFFGLGFETTMPTTAITLQQ
                     AKARDVQNFYFFCQHITLIPTLRSLLEQPDNGIDAFLAPGHVSMVIGTDAYNFIASDF
                     HRPLVVAGFEPLDLLQGVVMLVEQKIAAHSKVENQYRRVVPDAGNLLAQQAIADVFCV
                     NGDSEWRGLGVIESSGVHLTPDYQRFDAEAHFRPAPQQVCDDPRARCGEVLTGKCKPH
                     QCPLFGNTCNPQTAFGALMVSSEGACAAWYQYRQQESEA"
     gene            24672..25682
                     /gene="hypE"
                     /locus_tag="NBI74_001581"
     CDS             24672..25682
                     /gene="hypE"
                     /locus_tag="NBI74_001581"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417210.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation carbamoyl dehydratase
                     HypE"
                     /protein_id="HCD1931940.1"
                     /translation="MNNIQLAHGSGGQAMQQLINSLFMEAFANPWLAEQEDQARLDLA
                     QLVAEGDRLAFSTDSYVIDPLFFPGGNIGKLAICGTANDVAVSGAIPRYLSCGFILEE
                     GLPMETLKAVVTSMAETARAAGIAIVTGDTKVVQRGAADKLFINTAGMGAIPANIHWG
                     AQTLTAGDVLLVSGTLGDHGATILNLREQLGLDGELVSDCAVLAPLIQTLRDIPGVKA
                     LRDATRGGVNAVVHEFAAACGCGIELSEAALPVKPAVRGVCELLGLDALNFANEGKLV
                     IAVERNAAEQVLAALHSHPLGKDAALIGEVVERKGVRLAGLYGVKRTLDLPHAEPLPR
                     IC"
     gene            25756..27834
                     /gene="flhA"
                     /locus_tag="NBI74_001582"
     CDS             25756..27834
                     /gene="flhA"
                     /locus_tag="NBI74_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase transcriptional activator
                     FlhA"
                     /protein_id="HCD1931941.1"
                     /translation="MSYTPMSDLGQQGLFDITRTLLQQPDLASLCEALSQLVKRSALA
                     DNAAIVLWQAQTQRASYYASREKDTPIKYEDETVLAHGPVRSILSRPDTLHCSYEEFC
                     ETWPQLATGGLYPKFGHYCLMPLAAEGHIFGGCEFIRYDDRPWSEKEFNRLQTFTQIV
                     SVVTEQIQSRVVNNVDYELLCRERDNFRILVAITNAVLSRLDMDELVSEVAKEIHYYF
                     DIDDISIVLRSHRKNKLNIYSTHYLDKQHPAHEQSEVDEAGTLTERVFKSKEMLLINL
                     HERDDLAPYERMLFNTWGNQIQTLCLLPLMSGDTMLGVLKLAQCEEKVFTTTNLNLLR
                     QIAERVAIAVDNALAYQEIHRLKERLVDENLALTEQLNNVDSEFGEIIGRSEAMYSVL
                     KQVEMVAQSDSTVLILGETGTGKELIARAIHNLSGRNNRRMVKMNCAAMPAGLLESDL
                     FGHERGAFTGASAQRIGRFELADKSSLFLDEVGDMPLELQPKLLRVLQEQEFERLGSN
                     KIIQTDVRLIAATNRDLKKMVADREFRSDLYYRLNVFPIHLPPLRERPEDIPLLAKAF
                     TFKIARRLGRNIDSIPAETLRILSNMEWPGNVRELENVIERAVLLTRGNVLQLSLPDI
                     ALPEPETPPAATVVAQEGEDEYQLIVRVLKETNGVVAGPKGAAQRLGLKRTTLLSRMK
                     RLGIDKSALI"
     gene            27879..28307
                     /locus_tag="NBI74_001583"
     CDS             27879..28307
                     /locus_tag="NBI74_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012134844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TOBE domain-containing protein"
                     /protein_id="HCD1931942.1"
                     /translation="MAVSARNQLTGTVSAVAMGAVNDEVELTLAGGAKLVAIVTHSSQ
                     QALGLAKGKEAIALIKAPWVTLATEDCGLKFSARNQFAGSVSTITEGAVNATVHIKTD
                     AGFEIVAVVTNESQDEMKLTTGSRVIALIKASAILIATKA"
     gene            complement(28346..28690)
                     /locus_tag="NBI74_001584"
     CDS             complement(28346..28690)
                     /locus_tag="NBI74_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide-stimulated promoter family
                     protein"
                     /protein_id="HCD1931943.1"
                     /translation="MSGKRISREKLTIKKMIDLYQAKCPQASAEPEHYEALFAYAQKR
                     LDKCVFGEEKPACKQCPVHCYQPAKREEMKQIMRWAGPRMLWRHPILTVRHLIDDKRP
                     VPELPEKYRPKK"
     gene            28976..31537
                     /gene="mutS"
                     /locus_tag="NBI74_001585"
     CDS             28976..31537
                     /gene="mutS"
                     /locus_tag="NBI74_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HCD1931944.1"
                     /translation="MSTIENFDAHTPMMQQYLKLKAQHPEILLFYRMGDFYELFYDDA
                     KRASQLLDISLTKRGASAGEPIPMAGIPYHAVENYLAKLVNQGESVAICEQIGDPATS
                     KGPVERKVVRIVTPGTISDEALLQERQDNLLAAIWQDSKGFGYATLDISSGRFRLSEP
                     ADRETMAAELQRTNPAELLYAEDFAEMSLIEGRRGLRRRPLWEFEIDTARQQLNLQFG
                     TRDLVGFGVENAPRGLCAAGCLLQYAKDTQRTTLPHIRSITMERQQDSIIMDAATRRN
                     LEITQNLAGGAENTLASVLDCTVTPMGSRMLKRWLHMPVRDTRVLLERQQTIGALQDF
                     TAELQPVLRQVGDLERILARLALRTARPRDLARMRHAFQQLPELRAQLENVDSAPVQA
                     LREKMGEFAELRDLLERAIIDTPPVLVRDGGVIASGYNEELDEWRALADGATDYLERL
                     EVRERERTGLDTLKVGFNAVHGYYIQISRGQSHLAPINYMRRQTLKNAERYIIPELKE
                     YEDKVLTSKGKALALEKQLYEELFDLLLPHLEALQQSASALAELDVLVNLAERAYTLN
                     YTCPTFIDKPGIRITEGRHPVVEQVLNEPFIANPLNLSPQRRMLIITGPNMGGKSTYM
                     RQTALIALMAYIGSYVPAQKVEIGPIDRIFTRVGAADDLASGRSTFMVEMTETANILH
                     NATEYSLVLMDEIGRGTSTYDGLSLAWACAENLANKIKALTLFATHYFELTQLPEKME
                     GVANVHLDALEHGDTIAFMHSVQDGAASKSYGLAVAALAGVPKEVIKRARQKLRELES
                     ISPNAAATQVDGTQMSLLSVPEETSPAVEALENLDPDSLTPRQALEWIYRLKSLV"
     gene            31643..32299
                     /gene="pphB"
                     /locus_tag="NBI74_001586"
     CDS             31643..32299
                     /gene="pphB"
                     /locus_tag="NBI74_001586"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase"
                     /protein_id="HCD1931945.1"
                     /translation="MPSTRYQKINAHHYRHIWAVGDIHGDYQLLQSRLHQLSFCPETD
                     LLISVGDNIDRGPESLNVLRLLNQPWFISVKGNHEAMALEAFETGDGNMWLASGGDWF
                     FDLNDSEQQEAIDLLLKFHLLPHIIEITNDNIKYVIAHADYPGKEYQFGKEIAESELL
                     WPVDRVQKSLNSELQKINGADFFIFGHMMFDNIQTFANQIYIDTGSPKSGRLSFYKIK
                     "
     gene            complement(32350..33117)
                     /locus_tag="NBI74_001587"
     CDS             complement(32350..33117)
                     /locus_tag="NBI74_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCD1931946.1"
                     /translation="MIPVERRQIILEMVAEKGIVSIAELTDRMNVSHMTIRRDLQKLE
                     QQGAVVLVSGGVQSPGRVAHEPSHQVKTALAMTQKAAIGKLAASLVQPGRCIYLDAGT
                     TTLAIAQHLIHMEPLTVVTNDFVIADYLLDNSNCTIIHTGGAVCRENRSCVGEAAATM
                     LRSLMIDQAFISASSWSVRGISTPAEDKVTVKRAIASASRQRVLVCDATKYGQVATWL
                     ALPLSEFDQIITDDGLPESASRALAKLDLSLLVAKNE"
     gene            33313..34221
                     /locus_tag="NBI74_001588"
     CDS             33313..34221
                     /locus_tag="NBI74_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HCD1931947.1"
                     /translation="MKTGSEFHVGIVGLGSMGMGAALSCVRAGLSTWGADLNSNACAT
                     LKEAGACGVSDNAATFAEKLDALLVLVVNATQVKQVLFGEKGVAQHLKPGTAVMVSST
                     IANADAQEIATALAGFGLEMLDAPVSGGAVKAANGEMTVMASGSDIAFERLAPVLEAV
                     AGKVYRIGAEPGLGSTVKIIHQLLAGVHIAAGAEAMALAARAGIPLDVMYDVVTNAAG
                     NSWMFENRMRHVVDGDYTPHSAVDIFVKDLGLVADTAKALHFPLPLASTALNMFTSAS
                     NAGYGKEDDSAVIKIFSGITLPGAKS"
     gene            34218..35480
                     /locus_tag="NBI74_001589"
     CDS             34218..35480
                     /locus_tag="NBI74_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001649192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxo-tetronate kinase"
                     /protein_id="HCD1931948.1"
                     /translation="MIKIGVIADDFTGATDIASFLVENGLPTVQINGVPTGKMPEAID
                     ALVISLKTRSCPVVEATQQSLAALSWLQQQGCKQIYFKYCSTFDSTAKGNIGPVTDAL
                     MDALDTPFTVFSPALPVNGRTVYQGYLFVMNQLLAESGMRHHPVNPMTDSYLPRLVEA
                     QSTGRCGVVSAHVFEQGVKAVRQELARLQQEGYRYAVLDALTEHHLEIQGEALRDAPL
                     VTGGSGLAIGLARQWAQENGNQAREAGRPLAGRGVVLSGSCSQMTNRQVAHYRQIAPA
                     REVDVARCLSTETLAAYAHELAEWVLGQESVLAPLVFATASTDALAAIQQQYGAQKAS
                     QAVETLFSQLAARLAAEGVTRFIVAGGETSGVVTQSLGIKGFHIGPTISPGVPWVNAL
                     DKPVSLALKSGNFGDEAFFSRAQREFLS"
     gene            35477..36115
                     /locus_tag="NBI74_001590"
     CDS             35477..36115
                     /locus_tag="NBI74_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HCD1931949.1"
                     /translation="MSDFAKVEQSLREEMTRIASSFFQRGYATGSAGNLSLLLPDGNL
                     LATPTGSCLGNLDPQRLSKVTADGEWLSGDNPSKEVLFHLALYRNNPLCKAVVHLHST
                     WSTALSCLEGLDSNNVIRPFTPYVVMRMGNVPLVPYYRPGDKRIAQDLAELAADNQAF
                     LLANHGPVVCGESLQEAANNMEELEETAKLIFILGDRPIRYLTAGEIAELRS"
     gene            36120..36896
                     /locus_tag="NBI74_001591"
     CDS             36120..36896
                     /locus_tag="NBI74_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HCD1931950.1"
                     /translation="MPRFAANLSMMFTEVPFIERFAAARKAGFDAVEFLFPYDYSPQQ
                     IQQQLVQNHLTLALFNTAPGDINAGEWGLSALPGREHEARADIDLALEYALALNCEQV
                     HVMAGVVPAGEDAERYRAVFIDNLRYAADRFAPHGKRILVEALSPGVKPHYLFSSQYQ
                     ALAIVEEVARDNVFIQLDTFHAQKVDGNLTHLIRNYAGKYAHVQIAGLPDRHEPDDGE
                     INYPWLFRLFDEVGYQGWIGCEYKPRGLTKEGLGWFDAWR"
     gene            36985..38349
                     /locus_tag="NBI74_001592"
     CDS             36985..38349
                     /locus_tag="NBI74_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family transporter"
                     /protein_id="HCD1931951.1"
                     /translation="MSTITLLCIALAGVIMLLLLVIKAKVQPFVALLLVSLLVALAAG
                     IPAGEVGKVMIAGMGGVLGSVTIIIGLGAMLGRMIEHSGGAESLANYFSRKLGDKRTI
                     AALTLAAFFLGIPVFFDVGFIILAPIIYGFAKVAKISPLKFGLPVAGIMLTVHVAVPP
                     HPGPVAAAGLLHADIGWLTIIGIAISIPVGIVGYFAAKIINKRQYAMSVEVLEQMQLA
                     PASEEGATKLSDKINPPGVALVTSLIVIPIAIIMAGTVSATLMPPSHPLLGTLQLIGS
                     PMVALMIALVLAFWLLALRRGWSLQHTSDIMGSALPTAAVVILVTGAGGVFGKVLVES
                     GVGKALANMLQMIDLPLLPAAFIISLALRASQGSATVAILTTGGLLSEAVMGLNPIQC
                     VLVTLAACFGGLGASHINDSGFWIVTKYLGLSVADGLKTWTVLTTILGFTGFLITWCV
                     WLVI"
     gene            complement(38492..39358)
                     /locus_tag="NBI74_001593"
     CDS             complement(38492..39358)
                     /locus_tag="NBI74_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020231162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HCD1931952.1"
                     /translation="MSQPYILQKIGERTYYVQRYFYSTTFYVGDKGVLLFDAPEGRGK
                     YLLQAIRDVTPLPVTALVYSHYHVDHIGDSPFWNDEAKKEGVNLRIIASKATAEKMQF
                     MNSRLPVATQVLSKKDDQFKFEKQTIELHRFVKAGHTDDHSVWLLKQEKVAHSPDLLN
                     PDQLPMMGFAVSDTLVYHDSNLRQVEMLDWKYFIGGHGNIGSHDDFKFQRQFLNDLRD
                     TTIKVRKEESFGKFMNKTANNHADFARAQREAIIKKVTEVLRPKYGHMYGYDASMPAN
                     IEMAIRLVGSYY"
BASE COUNT         9510 a        10562 c        10351 g         9057 t
ORIGIN      
        1 gaaggagaga acaatgatag aaaccattac tcatggcgca gagtggttca tcgggctgtt
       61 ccagaaaggc ggagaagtgt ttaccgggat ggtaaccggc attcttccgc tgttaattag
      121 cctgctggtt atcatgaacg cattgattaa ttttatcggt cagcatcgta ttgaacgatt
      181 tgctcaacgt tgcgccggta accctgtttc ccgttatctg ctgctaccgt gcattggtac
      241 gtttgtcttt tgtaacccga tgaccctaag cctgggtcgc tttatgccgg aaaagtacaa
      301 acccagctat tacgcggcgg cctcttatag ctgccactca atgaatggcc tcttccccca
      361 tatcaaccct ggcgaactgt ttgtttatct cggcattgcc agcggtctga cgacgctgaa
      421 tctgccactt ggcccactgg cggtgagcta tctgctggtt ggtctggtca ccaacttctt
      481 ccgcggctgg gtaaccgatc tgaccaccgc cattttcgag aaaaagatgg gcattcaact
      541 tgaacaaaaa gttcgtctgg caggagcaac atcatgacac gcattcggat cgaaaaagga
      601 acgggtggct ggggcggccc gcttgagctg gaagccacgc cgggcaaaaa aatcgtctat
      661 atcaccgccg gtacccggcc tgcgattgtt gacaaactgg cacagcttac tggctggcag
      721 gctattgacg gatttaaaga aggtgaaccc gcggaggcgg aaattggtgt cgcggtaatc
      781 gactgtggcg gcacattacg ctgcggcatc tatccgaaac ggcgtattcc caccattaat
      841 atccactcga cgggcaagtc tggcccgctg gcgcagtaca ttgtggaaga tatttatgtc
      901 tctggcgtaa aagaagaaaa catcactgta gtgggtgagg cgacaccaca accctcttcc
      961 gtgggccgtg actatgacac cagcaagaaa atcaccgaac aaagcgatgg tttactggcg
     1021 aaggtgggaa tgggtatggg ttctgccgtt gccgtgttgt ttcaatctgg tcgtgacacc
     1081 atcgacactg tattaaaaac cattctgccg tttatggcgt tcgtctcggc gcttatcggc
     1141 atcattatgg cttccggtct tggcgactgg attgcccacg gtcttgctcc gctggcgagc
     1201 catccactgg gtctggtcat gctggcgctc atctgctcct tcccgctgct ttcacctttc
     1261 ctcggcccag gcgcagttat cgcacaggtt atcggcgtat tgattggcgt gcagattggt
     1321 ctcggcaata ttccgccgca tctggcttta ccggcactgt ttgccatcaa cgcgcaggcg
     1381 gcctgcgact tcatcccggt cggtttgtcg ctggcggaag ctcgtcagga cacggttcgc
     1441 gtcggtgtcc cttctgttct ggtgagccgc tttttaaccg gcgcgccaac tgtactgatc
     1501 gcctggtttg tctccggttt tatctatcaa tagaggctga aacatgaccg ttatttatca
     1561 gaccaccatc acccgtattg gcgcgagtgc cactgacgcc ctcagcgacc agatgctcat
     1621 cacctttcgt gaaggcgcgc cagcggatct cgaagagtat tgcttcattc attgccacgg
     1681 cgagttgaaa ggtgcactcc atcccggttt gcaatttacg ctcgggcggc atcgctatcc
     1741 ggtgaccgcc gttggcagcg tggcggaaga caaccttcgc gaactgggtc atgtcaccct
     1801 gcgcttcgat ggtttaagcg aagcggaatt tccgggcact gtccatgtgg caggccctgt
     1861 acccgacgat atcgcgccgg gatcggtttt gaagtttgaa tctgttaagg agtaaaaaat
     1921 gaatcaggtt gccgttgtca tcggtggtgg gcaaacctta ggcgcgttcc tgtgccacgg
     1981 tctggctgcc gaggggtatc gcgtggcggt tgtcgatatt cagagcgaca aagccgcaaa
     2041 tgtggcacaa gaaattaacg ccgaatatgg tgaaggaaca gcgtacggtt ttggtgctga
     2101 cgccaccagc gagcaaagcg ttctggcgct ttctcgtggg gtagatgaaa tctttggtcg
     2161 cgtggatttg ctggtctaca gcgccggaat agcaaaagca gcctttatca gcgatttcca
     2221 gcttggagat tttgaccgtt cgctacaggt gaatctggtg ggttatttcc tgtgtgcccg
     2281 tgaattttcg cgtttgatga ttcgcgacgg gattcagggt cgcattattc agatcaactc
     2341 gaaatccggc aaagtgggca gcaaacacaa ctctggctac agcgcagcga aatttggtgg
     2401 cgtcgggctg actcaatcac tggcgctgga tctggcggag tacggcatta cggtgcattc
     2461 gctgatgctc ggtaacctgc tgaaatcgcc tatgttccag tcactgttgc cacaatacgc
     2521 gaccaagctg ggtatcaaac cggagcaagt cgagcagtat tacatcgata aagtaccgct
     2581 caaacgcggc tgcgattatc aggatgtgct gaatatgctg ctgttctacg ccagtcctaa
     2641 ggcgtcgtac tgcactggtc agtcgatcaa tgtcaccggc ggtcaggtga tgttctgatc
     2701 aacagcggag atcttttaag gatctccgcg cgactaatag aatgcctgat gcgctacgct
     2761 tatcaggcct acaggacttc cgccactaca tcacggagaa gttatggtat ccgcactcat
     2821 caccgtcgcc gttatcgcct ggtgtgcgca actggcctta ggcggttggc aaatttctcg
     2881 ttttaaccgt gccttcgaca cactatgcca acaagggcgc gttggcgtgg gacgttccag
     2941 cgggcgcttt aaaccacggg tcgtggtcgc catcgcgctg gacgatcagc agcgcatcgt
     3001 cgacaccttg tttatgaaag gactgaccgt cttcgcccga ccgcaaaaaa ttcccgcaat
     3061 taccggcatg catgcgggtg atttacagcc cgatgtgatc tttccccatg atccactatc
     3121 acagaatgct ctatcattgg cgcttaaact gaaacgtgga taatttcgtt gtgaatgtta
     3181 ctagcttgcg aagttatcat tttgaaacct aaatcaggta atcacgccca tgaaacctcg
     3241 tcagcgtcag gccgccattc tggagtatct gcaaaagcag ggtaaatgct cggttgaaga
     3301 attggcgcaa tactttgaca ccacaggcac aaccattcgc aaagatctgg tcattctgga
     3361 acatgccgga accgtcattc gtacttatgg cggcgtggtg ttgaacaaag aggaatccga
     3421 tccgcctatc gatcataaaa cgcttatcaa cacccacaag aaagagctga ttgcagaagc
     3481 tgccgttggt tttattcatg atggcgactc aataattctt gatgctggca gtaccgtgtt
     3541 gcagatggtg cctctgctct cacgttttaa taacatcacg gtgatgacca acagtctgca
     3601 catcgtcaat gcgctatccg aactggataa cgaacaaact atcctgatgc caggcggaac
     3661 atttcgtaaa aaatcggcct catttcacgg gcaactggca gagaatgcct tcgagcattt
     3721 caccttcgat aaattgttta tgggcaccga cggcatcgat ctcaatgcgg gcgtaaccac
     3781 gttcaacgag gtttataccg tcagtaaggc gatgtgcaat gccgcgcgcg aagtgatttt
     3841 gatggctgac tcatcgaagt ttggtcgtaa aagccccaac gtggtttgta gtcttgaaag
     3901 cgtcgataag ctgattaccg acgcaggtat cgatccagcg tttcgtcagg cgctggaaga
     3961 aaaagggatc gacgtgatca taaccggaga gagcaatgag tgaagcacta ctgaacgcgg
     4021 gacgtcagac gttaatgctg gagttgcagg aagcaagtca tttaccggaa cgtctgggcg
     4081 atgatttcgt tcgcgccgcc aatatcatcc tgcactgcga aggcaaagtg gtggtttcgg
     4141 gaattggcaa atcgggccac attggtaaga aaatcgccgc aacgcttgcc agcaccggca
     4201 caccggcttt ttttgtccat ccggcagaag cactgcacgg cgatctgggg atgattgaaa
     4261 gccgcgatgt gatgctgttt atctcttatt ccggtggcgc gaaggaactg gatctcatta
     4321 ttccgcgtct ggaagacaaa tctatcgcgc tgctggcaat gaccggaaaa ccgacgtcac
     4381 cgctgggcct ggcggcgaaa gcggtgctgg atatctccgt agaacgcgaa gcctgcccga
     4441 tgcaccttgc gccgacctcc agcaccgtga ataccctgat gatgggcgac gcgctggcga
     4501 tggcagtcat gcaggcgcgt ggttttaatg aagaagattt tgcacgctcc cacccagccg
     4561 gggcactggg cgctcgcttg ctgaacaaag tgcatcatct tatgcgtcgt gacgatgcca
     4621 tcccgcaggt agcgttaacc gccagcgtga tggacgcgat gctggaactt agccgcaccg
     4681 gtttaggtct ggtggcagtg tgcgatgatc aacgactggt gaaaggcgtc tttaccgacg
     4741 gtgatttacg tcgctggctg gttggcggcg gcgcactcac cacgccagtc aatgaagcga
     4801 tgacggtcga cggcaccacg ttgcaatcgc aaagccgtgc catcgacgcc aaagaaatcc
     4861 tgatgaaacg aaaaatcacc gccgcaccgg tagtggatga aaacggcaaa ctcaccggcg
     4921 caattaacct gcaggatttc tatcaggccg ggattattta atccttcagc cccagacgtt
     4981 tcgccagccg atgcaggttg gcgacgtcgg tttccagcat ccgcgcacag gcggcccagt
     5041 tgtgatgatt ttgcgccagt gcctgacgaa tggtttcacg ctggaacgct tctgtcgctt
     5101 cacgcagatt ttgcttaaca acgggcaccg ccgccgcttc tggcggcggc aacgtcacct
     5161 caggaaaagc aaaatgttgc gcctcaagaa tcacttcatc gccgctgcgg gtggctctcg
     5221 acaatactac cgcccgatga atagcatgtt ccagttcgcg cacgtttccc ggaaaattgt
     5281 agtgttgcag taaatttcgc gctccggcac ttaataccac gcgggagagc cccagccgca
     5341 aacgacactg ctcgcagaaa taccccgcca gcagaatgac atcatcgccc cgctcacgca
     5401 gcggcggcac tgaaagtgga aacacgctca ggcggtggaa caagtcagcg cgaaatcgcc
     5461 ctgccagtac ctcttcgcgt aagtcacggt tagtcgccgc cagcacgcgc acatcgaccc
     5521 gcaaactgcg gtcatcgcca acgcgttgaa tatcgccata ctgcaacacc ctcagcagct
     5581 tggcctgcaa tgccaacgac aactcgccga tctcatcgag aaacagcgtg ccgttatccg
     5641 ccatttcaaa cttcccgctg cggttactga tagcaccagt aaacgcccct ttcacatgcc
     5701 cgaacaactc actttccgcc acactttccg gcagtgcggc acagttgaga tagaccagcg
     5761 gattcaccgc ccgtggcgag gcttcatgaa tcgctttcgc caccagctcc ttaccggttc
     5821 ccgtctcacc gctaattaaa acgttgagat cggatgccgc cacaatctca atctcttttt
     5881 tcaattgcgt catgcctggg gacaaaccaa tcatctgcgt ctgtttcacc gcttcaaacg
     5941 gcgcggcatc gcctggcagc atattctggc tttccagttg ttcaatcagt aacgcattgc
     6001 ttaacgctcc cgccgccagc gcagcaatca ggcgtaactc ttcgtcgctg aaaacatcga
     6061 actgatcggg ctgcatcccg tcgagcgtca aggcaccgat caggttttgc ccggcaaaca
     6121 acggcaaacc aacgcaggcg tgaaccttca gactctcctg ccctggaatc agaccgtcat
     6181 aaggatcggg caattcgctg tctgcgggaa agcgcaccac atcacccgcg cgggcaatcg
     6241 cttccagccg tggatgccct tccagcgcaa agcgtctacc gagtacatcc ttcgccagcc
     6301 cgtcgatggc aagcggaata aactgccgcg agtcgtaacg tagcaacgca gacgcatcgc
     6361 actccagcac ctgacgcagc gtagtgatca ggcgctgaaa acgatcctga tgaccaatcc
     6421 cacgctgcaa ttcgatggcg atattcgcca gcacatcaac ggaaaaactc atctttgcct
     6481 cactgtcaat ttgactatag atattgtcat atcgaccatt tgattgatag tcattttgac
     6541 tactcattaa tgagcataat tttatttata gagtaaaaac aattagataa aaaactggca
     6601 cgcaatctgc aatcagcaag acatcttttt agaacacgct gaataaattg aggttgctat
     6661 gtctattgtg gtgaaaaata acattcattg ggttggtcaa cgtgactggg aagtgcgtga
     6721 ttttcacggt acagaatata aaacgctgcg cggcagcagc tacaacagct acctcatccg
     6781 cgaagaaaaa aacgtgctga tcgacaccgt cgaccataaa ttcagccgcg aatttgtgca
     6841 gaacctgcgt aatgaaatcg atctggcgga tatcgattac atcgtgatta atcatgcaga
     6901 agaagaccac gccggagcgc tgaccgaact gatggcgcaa attcccgata cgccgatcta
     6961 ctgtacagcc aacgctatcg actcgataaa tggtcatcac catcatccgg agtggaattt
     7021 taatgtagtg aaaactggcg acacactgga tatcggcaac ggcaaacaac tcatttttgt
     7081 cgaaacgcca atgctgcact ggccggacag catgatgact tacctgacag gcgacgcggt
     7141 gctgttcagt aacgatgctt tcggtcagca ctactgcgac gagcatctgt tcaacgatga
     7201 agtggatcag actgagcttt tcgagcagtg ccagcgttac tacgccaata tcctgacgcc
     7261 gttcagccgc ctggtaacgc cgaaaattac cgagatcctg ggctttaact tgccagtcga
     7321 tatgatagcg acctctcacg gcgtggtatg gcgcgataac ccgacgcaaa ttgtcgagct
     7381 gtacctgaaa tgggcggcgg attatcagga agacagaatc accattttct acgacaccat
     7441 gtcgaataac acccgcatga tggctgacgc tatcgcccag gggattgcgg aaaccgaccc
     7501 acgcgtggcg gtgaaaattt tcaacgtcgc ccgaagcgat aaaaacgaaa tcctgaccaa
     7561 tgtcttccgc tcaaaaggcg tactggtcgg cacttctacg atgaataatg tgatgatgcc
     7621 gaaaatcgcc gggctggtgg aggagatgac cggattacgc ttccgtaaca aacgcgccag
     7681 cgctttcggc tctcacggct ggagtggcgg tgcggtggat cgtctttcca cgcgcctgca
     7741 ggatgcgggt ttcgaaatgt cgcttagcct gaaagccaaa tggcgaccag accaggacgc
     7801 tctggagtta tgtcgtgaac acggtcgcga aatcgcccgt cagtgggcgc tcgcgccgct
     7861 gccacagagc acggtgaata cggtggttaa agaagaaacc tctgccgcca cgacggctga
     7921 cctcggccca cggatgcaat gcagcgtctg ccagtggatt tacgatccgg caaaaggcga
     7981 gccaatgcag gacgttgcgc caggaacgcc gtggagtgaa gtcccggata acttcctgtg
     8041 cccggaatgc tctctcggca aagatgtctt tgacgaactg gcatcggagg caaaatgagc
     8101 aacggcattg tgatcattgg ttcgggcttc gccgcccgcc aactggtgaa aaatattcgc
     8161 aaacaggacg cctgtattcc attaaccctg attgccgccg acagcatgga tgagtacaac
     8221 aaacctgacc tcagccatgt tatcagtcag gggcaacgtg ccgacgacct tacccgccag
     8281 acggcaggtg aatttgccga gcagtttaat ctgcgcctgt ttccgcacac ctgggtaacg
     8341 gatatcgatg ccgaagccca tgtggtgaaa agtcagaata atcagtggca atacgacaag
     8401 ttagtgctgg caaccggtgc cagcgccttt gtcccgccag tgcccgggcg tgagttaatg
     8461 ctgacgttaa atagtcagca agagtatcgc gcctgtgaaa cacaactgcg ggatgcccga
     8521 cgcgtgttga ttgttggcgg tggtttgatt ggtagcgaac tggcgatgga tttttgtcgg
     8581 gcaggcaaag cggtcacgct aatcgacaac gctgccagta ttctggcgtc gttaatgcca
     8641 ccggaagtca gcagccgctt gcagcatcgg ttgacggaga tgggcgttca tctgctgtta
     8701 aaatctcagt tgcaaggact ggaaaaaaca gattctggca ttctggcaac gctggaccgc
     8761 cagcgctgca tcgaagtgga tgcggtaatt gccgccaccg gactgcgccc ggaaaccgcc
     8821 ctggcacgac gcgccgggct gacgattaat cgcggcgttt gcgtcgatag ttatctgcaa
     8881 accagtaatg ccgatattta tgcgctgggc gattgcgcgg aaattaacgg tcaggtattg
     8941 ccgttcctcc agccgattca acttagcgcg atggtgctgg caaaaaatct tctcggcaat
     9001 aacacgccgc tgaaactccc ggcgatgctg gtgaaaatca aaacgccgga attaccgctg
     9061 catctggcag gcgaacccca gcgtcaggat ttacgctggc aaattaatac cgaacgccag
     9121 ggaatggtgg cgcgcggtgt tgacgatgct gaccagcttc gcgcctttgt ggtcagtgag
     9181 gatcggatga aagaggcatt tggattgttg aaaacgttgt cgatgtaggc gagctacagt
     9241 gccccaaatg tcggatgcgg cgctgacgcg tcttatccga cctacaggga acgcatgtgt
     9301 tgatcacata atacgtttat gtcgcattgc actgtcccaa aatgtcggat gcgacgctag
     9361 cgcgtcttat ccgacctgcg gggatgaata tgtaggccgg ataaggcgtt cacgccgcat
     9421 ccggcaatgg tgtccaaatg caacacgctt tatccgttct ggacttcacc cgctaaccaa
     9481 cgcgccgcag caataacccc ctgccccaga gacaacccgc catcgcccgc aggtaaactc
     9541 tgtggaaaga gcaatgtgaa atcagcgaga taatgcgcca gacgtgcacg cagcaaacgg
     9601 ttatgaataa ccccgccgct aaataccagc gtagtgatac cacgcatcgt ggcctgctca
     9661 cgcatcaacg cggccccccc ctgcgccagc gcatcatgaa acgcccacgc acgttgatta
     9721 accggagcct gccagttcag ccactgctgc cagaaggtgg cgagatccag ttgattatcc
     9781 accagcggca tcgtcaccgg atgcgtcacg ccgtggcacg aagtcgccag cgcctccaga
     9841 gcacaagccg cttcaccttc ataacttaac gtggctggcg cacagcccag cgccgccgct
     9901 acggcatcga acaaccgccc acacgatgac gccagtggtg cattgattcc acgctcaatg
     9961 gcccgcgcca gcacgctcca gttttgctgt tgcacactta ccgtttcggg ataactctgc
    10021 cactccggca caaaacgcaa gcactgcgtc agcaggtttc gccacggctg ctttgcggca
    10081 agatcgccac cagcaagcgc cactgcaggc aagccgccca ggtgctcgca ttcacgatag
    10141 ttcacccgca ggcactctcc cccccacaaa gcgccgttct cccccatacc aataccgtcg
    10201 agtgtcaacg caatgacatc tccgccatcc agcggccagt gatgttctgc cagacatgcc
    10261 gctgcgtgag catgatgatg cagcaccgtt tgcgtcggca gattcatttc gcgcgcccac
    10321 tggctggaga catagcccgg atgcgcgtca tgcacaacgt attgcggagt gaaatcgtag
    10381 atgttttgca tcaggcgtaa cgcttcgcgc cactgcatct ggatgccatc gtcacttaaa
    10441 tcgcccagat gctgactcaa caccgcttgt tcgccgcgca ccaggcagaa ggtatttttc
    10501 agatccgcgc cgagacacag cacaggcgga acatttttaa agcccggagg caaagccagc
    10561 gcatccggca catacccccg cgaacggcgc agcatttcgc cgctttcgcg caccaccgaa
    10621 tcatccatcc gctgcacgat gtcgcggtta tgtatcaaga atccgtcggc aatgccctgc
    10681 aaatccgcca gcgcctgttc gttgctgata gccggtggtt taccgctcag gttgccggag
    10741 gtcatcacca gcgggcattg cagttcctgt aacagcaaat gctggagcgg gttcgcaggc
    10801 aacattaccc cgacttcgtt aaggccaggg gcgatatcat cacaaagctc aggtacatat
    10861 tttttatcca ccagcacaat cggcgcggcg ggcgtggtaa gcaactggcg cgcagcgtct
    10921 ggtaaaccgt cagccactgg caacatgacc gccagcggtt tcgccgggcg atgtttgcgc
    10981 gcccgaagtg tcgccaccgc gttactgtta cgtgcatcgc aggcaagatg aaatccgcca
    11041 atccctttga tggcgacaat gttgcccatt tttaactgtg cgatagctgc ctgtaatgcc
    11101 gcctcttgtt ctgcatgttc accatgactt acccattcaa gatgggggcc acactccggg
    11161 caggccaccg gctgggcgtg gaagcgacga tcgagcgggt cacagtactc tttgtcacaa
    11221 gccggacaca gcggaaacgc cgccatcacg gtaaacgggc ggtcgtaagg catggcgcga
    11281 ataatggtga aacgcgggcc acagtgggta cagttgataa acggataacg ataacgccgc
    11341 tcgcccgggg tattcatttc ggcaaggcaa gcagggcaag tcgcggcatc gggaactatt
    11401 tgcgtattca tagcgccgcc tgtgctctgg cggatagtga actcggtggg cagttgtgac
    11461 cagataaacg gctcacgctc gacgctatca atacgcgcca gcggcggaca atgctgatgc
    11521 aattgaacaa gaaaggtttc cggctcttcc agcagccgga cttctacgcc atcgccgtca
    11581 ttacagacat cgccgtgaag atttaattgc tgtgccagtt gccagacaaa cggacgaaaa
    11641 ccgacgccct gcactttgcc acgaatacgc agttggacac cgcaagatgt gttttttgcc
    11701 attgagttat tcccgccatc atgaattgcg taacccgccc agccggacac gacagcgtcg
    11761 catccggcag tcacaggtcg gcgatactgc ccgcttcgta ttctacgaat atttccggga
    11821 attcctttga taccagaaca gttctgtaag atttttagaa catcagcgcc gtgcggcggc
    11881 gtttttctgc gctcagttgt tcaagtttat tacgatcgac acaaatcagc gcatgggtcg
    11941 ggcaagccgc catacacgcc gggccgtctt cacgatggtt gcacaggtcg catttattgg
    12001 cttcggcttt gtcagcccgt acattcagcc ctgcgccgct gttgcgaatc accggacgca
    12061 ccaccacttc catcgcacca tatgggcaag ccacaacgca ggttttgcaa ccaatacaac
    12121 gttcctgcat gacatgaaca aaccctttat cacggctgat agcaccattc gggcagacgt
    12181 tagcgcacgg tgcatcttca cactgacggc agactgtcgc cgtggaaatg ttcacacctt
    12241 taatgacatg gattcgcggt aaaaatgttt ccggggtcag cgatgcacag tcctgatttt
    12301 cctgatgaga aaccacgcac gccacttcac aggtacggca accaatacat ttactcgcgt
    12361 cagcaatgat gaaacggttc atcaaattct ccagcaataa cagttaatgc gccgatacat
    12421 tcacaaatca tgccagtttt taatttactg ttatttaagg aaattaaatt ctgtactaca
    12481 ggaaaaacga tgtcatcgac actagtgtcg atgacatgtg atgacagtat ttatcgtagt
    12541 aaggcagaga gaatccgttt tctaatgaat attttatcgt tgccagtcca catctgggta
    12601 cttttttccg acaagtcgtt ctggtggtat aaaaaagtaa ttgccagtaa cggggaggat
    12661 ttaagggaag caaccgtgcc ggatacggcg tgaacaacga ccactttgtc agctatccat
    12721 atgattgtat tttgaggttc atcgcagatt agcgatgaac ctttattgaa taatactatt
    12781 tcgactgaaa taacaggtaa ggatacaaaa tgggttcatg gggaatggag gctctcgaaa
    12841 gcgacgaagg gctggatttg attaattggg tagaggaaca actgcaagat gattcgacct
    12901 ttgatgccga gtcaattgtt caacgcttaa gccagcatga agatttgttc ggatttcagg
    12961 gagatgaaga atttctctac gataattacg ataataatgt tatcggtctc gttgaactta
    13021 tcattcaaaa agcagccggg aaaaaaatca catcgagtaa acaaattgat caactcgacg
    13081 gttatcagct cacgtcgact ttcagcaaaa aactccaggg ccgtttacaa acaatcgacg
    13141 acacacatga gtggataatg cttttcgagg ggcgagcacg cgaaaaagca aaagcatatc
    13201 tgattgagct tactgataaa ttaagagtag taaagacaac agcctgaatg acaaacaagg
    13261 gcgcgcaaaa ttaagaatct gcgccctttc aaagcccgat aggcgtagcg catcaggcga
    13321 tttagcgtct gccatcagtc aaagaaacca ttccggctta acgccaaatc gggctgaaag
    13381 tgccttaatg tgggatatcg tcagggaacg ttgtccagat aaaatctggc tgaccaggga
    13441 tttagagccg atttcgttct tcaaatctgc ataggtcaga ttatgttgat caatcagcgt
    13501 gcgaagtaac gccacaccaa caggcattgc tgcaacggct ttgtcgaact ctgcaaactt
    13561 ttcgttattg ttttcatatt ctgcaatgcg gctcgccaga aagtcgataa gtggattagt
    13621 atcatcttct tcaatcaaat attcgaccag ttccagtgct tcacggtaat catcttctga
    13681 gtcactgcct cccagaaagg ggactgcatt gaccagttct cttgttgctt taacagcgcg
    13741 tgcagcatta gcagtcattc tttgttctct ctgtagtaac gtgtcagacg cccacttttc
    13801 taaaatgcca ggtttgctcg gcatcataag gcatggtcag gagaggtttc agcgcccgct
    13861 tttgcgcgta ttcgctgtca ctcattggca gatattcggt aaagctggtg gcctgtgcag
    13921 gcagcactaa gctcaataaa aaagcgcaca tccctgtttt gatgttcaat tccctgaatt
    13981 ccctaatgaa gaattattct tccaccgcta actgcgcgaa gccgccattt ccttcccagc
    14041 cttccagccg ctgatacacc gtttccaccg catctttaat cggctgagtc atcgggtagt
    14101 aaaagccgac gatatccggc tggatgccaa ggaaaatcac ttcgccaata tcttctttca
    14161 actggtcgat aagatagttc agcggcatgt tatgggtagt catcataaac atctcggcga
    14221 tgtcagccgg gtcgatgatg cggatctcgc cagggtttag ccccatatcc gtggcgtcga
    14281 caatcagcag ccgtgtcggg cgcagttcgc ggatagcgac aatgtcgttt tccggtgcgc
    14341 taccgccgtc aatcactacc cagttacctt tcggcgcggc ggcgcacttt tccgccagta
    14401 gcgggcctgc gccatcatcg cccatcatgc tattgccaac acaaagtaaa acgtcagtca
    14461 cgcagtctcc tcaccatcaa atagatggcg ttttcctgat gaatatcatg cagcatactt
    14521 aacagcattg tactccattc cttctgctgc gaagtttgcg ctgcccgggc tttatcgaat
    14581 gcgttggcca gcatcggcac atggttaatg tcgatgacga tctcaccata tttcggcacg
    14641 ccttccattt tacggcgggc ttcgctgccc gcctccagag tggcaatcca tgccagatag
    14701 tcatcccacg ggcaggtaag tgccgcttcc aggcaatcga taaccccaag gtggtgacca
    14761 atcgccaggc tgtaatagac cacctgctgc gcctcggcgg gcgtggcatc gttctcatca
    14821 ataaatttac ggctcagttg actgaacacc accttttcac tcatcggata cgcgcctctt
    14881 caacaacatg attcagatgg ctgacaatct cgttcagacg cggatcgttt tccgcttcca
    14941 gccagcgtgc aacctgttct tcgccctgcc ctaactgtgt caggaaatca tcggcaatct
    15001 ggcgaccgta acgataaccc gccagacgac gcgcttcacg gtccactttc acacgcaacg
    15061 gttgcaccat atcaggatgt agaatgtctg ccggctgctc atcttgctca cccggcccac
    15121 gggcgtggat tttctgctcc agcaggccga gcgccatcgc aaagccgtac agcgtggcgg
    15181 caggcgttgg cgggcagcca gggatgtaga catctaccgg aacgatttta tccgtaccgc
    15241 cccacacgca gtagagatcg tggaagatcc cgccgctgtt accgcaggca ccgtaagaga
    15301 tacagatttt cgggtccggc gcggactgcc acgcacgcag cgcaggggat cgcattgcac
    15361 gggtgaccgc gccggtaaac agtaaaatat ccgcatgacg cggtgacgga acgactttaa
    15421 tgccgaagcg ttctgcatcg aacagcggcg aaagcgtggc gaaaatttcg atttcgcaac
    15481 cgttacagcc gccgcagtcc acgcgataaa cgtaggcaga acgtttgatt tttttcagta
    15541 acgacgcctt catgctggcg atggattcat ccaccgtcat cgggaccgga atgccgttgg
    15601 cgtcacgggg gcctaataaa ttgctcatca gatggcctct ttcatatggc gagtcagttc
    15661 aatacggtcg gacggcacca ggcatttctg gcgcttacat tccgggcaag tctcaaagct
    15721 ttcgcggtgg ttttccgcgc ggctgtcgcc gttgtgctta agcagcgcaa tggcgtagtc
    15781 gatctctttc tggacggcga aaggacgatt gcagacgcgg cagttgcaca gcgcgaagcg
    15841 ggactgttgc aggaagtctt ctttcttcca caccgccagt tcgtattctt gcgacagctt
    15901 aatcgccgcc gtcgggcaga cttcttcgca gcgtccacag aagatgcagc gtccaagatt
    15961 gaactgccac gccagctctc ctgtggcgag gtcagtttca accgttaagg cgtttgacgg
    16021 gcaggcattg acgcaggccg cacagccgat gcactgctgc gggttctgct ctggcttacc
    16081 acggaagttt ttatcaaccg caatcggctc cagcggataa gacgaagtcg ccgtgccggt
    16141 tttgatgact tttttgataa aggtaaacat ggcgattcct tatttcagcg gcgagttttt
    16201 acgctcaatg ctgtagcgct cgagttcttt gtacggcacc actttgctct tcttcttacg
    16261 tacatcgacc acggtcatgc ggtcggtaca ggagtagcaa gggtccaggc taccgataat
    16321 cagcggcgca tcggaaacgg tattgccacg cagcatgtag cgcagggtcg gccagttcgc
    16381 gtaggtcgcg gcacggcagc gccagcggta cagcttctgg ttgtcgccgg tcatgctcca
    16441 gtggatatcg tcgccgcgcg gcgcttcggc aaagcccagc gcgaaacggt gcggaatgta
    16501 ggtaaagcct tccaccatca gcgggccgcc cggcaggtta tccagaccgt aatcgatcat
    16561 attcagcgcg gtatagactt cgttgatacg cactttcaga cgggaaataa cgtcgcagcc
    16621 ctgctcgctg tggacttcca ttggcagcag gccataacca acaaacgggt gatcggcgcg
    16681 ggtatcgcga gcgtgtccgc tggcgcggac catcgggcca acgttactga aatcgcgggc
    16741 aatttccggg tccagacgac caatgccgac ggtgcgctgt tccatgttcg gtgtgctcag
    16801 cagcacatcc accagctcct gcacttcacg acgcatctgt tgcgccagct ggcgggtctg
    16861 aatcatgtcg tctttcagca gatcgcgacg aatcccgccg atcaggttca ggccgtaggt
    16921 tttacgcgca ccggtaagga tctctgccat tttcatggag gtttcacgca cgcggaagaa
    16981 ctgcataaag ccggagtcaa agccggtaaa gtgacaggcc aggccgaggt tgagcagatg
    17041 cgagtgcagg cgttctacct ccagcagaat ggcgcggatc atctgcgcgc gttctggcac
    17101 ctgaataccc atcgcgtttt ccaccgacgt ggtataggcg gtgctgtgag caaagccgca
    17161 gatcccgcac acacggtcag agaggaaggt cacttcgttg taacccatac gggtttccgc
    17221 cagtttttcc atgccgcgat ggacgtagaa caggcggtag tcggcgtcga taatgttttc
    17281 accatcgacg aacagacgga agtgacccgg ttcgtcagaa gtgacgtgca gtggaccaat
    17341 cggcacgacg ttgtttttct tgtcgcccag ttcgttgatg aactcgtagg tttcagcatc
    17401 ggtagtcggt gccggacgct gacgataatc catgctgtct ttacgcagag gataaagttc
    17461 atccggccag tcatccggca gcaccagacg gcgttcatcc ggcagaccaa ccggaatcaa
    17521 accgtacata tcgcgcactt cacgctcgcc ccacaccgcc gccggaacgc gcggcgtcac
    17581 ggaaggatac tccggtttgt tggcgtcaac ttcaacgcga accgttaccc aacacttggt
    17641 gcccttctcc atcgacagca cgtaataaac ggcgtaatga ccattcagtt tgcgttcgtc
    17701 gttaccaaac agcaccgaca gccagccacc ctgcttgtag tagagaaact ccaccacttc
    17761 cggcaggtag ttcaccttta cggtcacggt cagctgatct ttggtctgcc aggcgtggtc
    17821 cagcacgacg ccaggaaatg cctcattcag cgcggcgaga taatgttgac ctaatttttc
    17881 ttcagacatg ctcaaactct ctttaatcac gccgccagca aggagacgaa cgctaaaaat
    17941 gcaaagccaa acccggccca ggtaatgcgc ggagtaatat caagacgcag acgggccatg
    18001 ctgttttcga acagcgcgat aaccaggacg ccaaccacca gtttgacgat ggcaatcacc
    18061 agcgccagca gcagcccacc gacggtgaag gtttccattt gtccccacgg aataaacacc
    18121 ccgacgaaca tctgcaacac caccagctgt ttcaggctga taccccattt catgacgcca
    18181 aagccgctgc cgctatactc agagagcgga ccttcctgca actcctgctc ggcttccgcc
    18241 aggtcgaacg gcagtttgcc catttcgata aaggtggcga atgcacaggc acaaagcgcc
    18301 agcaccagcg ggatgctctg gctcagtggc cagtgataaa cggtgtcggt gatgttgctg
    18361 atgttggtgg aaccggcaac ctgtgcggcg acccacagac caagcagcag catcggttca
    18421 accaacacgc caagcatcgc ttcacggctc gcgccgatag cggtaaacgg gctaccggta
    18481 tccagaccag aaatggcaaa gaagaaacgc gcgatggcga agagatacag taaggtgatc
    18541 aaatcgccca gttgcggcag cggagaaccg acggtcacca ccggcagcgc agtggcgata
    18601 gtcagcatga cgcccaccat cacatacggc gtcaggcgga acacccagcc ggaggcatcc
    18661 gggccaacgc tctggcggcc cagcagtttg ataatgtcgc gatactcctg caacacaccc
    18721 ggcccacgac ggttatgcag acgggcgcgt gctacgcggg taataccgga gagcagcggc
    18781 gcaacggcaa atagcaccag cgcctgaatt aacggatata aaacactcat tctcaggctc
    18841 ctcgtgaaac aataatcacc accagcaccg ccagttcaac cagcgccatc cggcggaaca
    18901 gcaacgcact cccctcgcac tgccagcccg gcaccagaga caccggattc agccatttgc
    18961 gtagtttcag caccggcgca aacgcctgtt tcaccggcat ggcaaaaccg tgagcggtaa
    19021 tcaccattga tttttcatga tcgtagccgc acacccaggc cgcgccgcgg gaacgcgacg
    19081 gcaaacgatc gcctttgcaa atcgccataa tgatgaatgg cagcagcggg caggcaatca
    19141 acaacaacgt gatcattggt tgagaaacgg tggtgttagc aggctccagc ggcagaggta
    19201 cagcagcaga gagcatcggc agtagccacg gcgcagcaac accgccaatt acgcagcaaa
    19261 tcgccagtgc cactacgctt acgctcatca ggagcggcga acaggtggcg ttttcggctt
    19321 ctttggtacg cggcgcgccg aggaaagtaa cgccataaac tttcgccata cacatcaccg
    19381 ccagcgcacc ggtaattgcc agccccacgg cgagcagcgg cccgagaaga cgggcaacaa
    19441 acgcgccact attgctcagt ttgaagaagg attgatagat aacccattcc ccggcaaaac
    19501 cgttcagcgg cggcagcgca gccattgcca tcagcccgac taacatggcg atggagataa
    19561 ccggcatttt cttgccaata ccaccgagtt tttcgatatc gcgatgaccg gtacggaacc
    19621 agacgctccc cgcaccaagg aacagtacgc tcttgaacag gctatggtta agcagatggt
    19681 acagaccacc aaccagacca agggcaatca gcgccggttg ttcgagcgcg atacccgtta
    19741 cgccagcgcc cagccccagc aggatgatgc cgatattttc cagggtgtgg taagccagca
    19801 gacgttggat attatgttcc atcaacgcat acagaccgcc gacgaatgcg gtgatcatgc
    19861 cgagtaccag cagtgcgatc ccccaccaca gcggtgcatt accgcccagt agtgacagag
    19921 ttaaaatgcc cagcaggcca attttcatga ctaccgtgga aaacagcgcg gcagctggcg
    19981 cagaggcgtt ggcatgtgcc tgtggcaccc agccgtgcag cggaataatc ccggccagca
    20041 ggccaaagcc aatcacaccg agcagccaga tatcggaacc gagcggcagc tgttgcatac
    20101 gcatatccag caggcgcaga tccagcgtgc cgtaacgctg ccacagcagc cagcaggcaa
    20161 tcgccagcag cagagtgcca agacgcccca gcgcaaacca cagtttgccc tctttgctgt
    20221 tgctggtgag gaacaccgca cacagggcca tgatttcggc cattaccacg aacatgccga
    20281 ggttgctggc gatgacggca cagacggcgg cagccatcaa catattgatc tgcaagccgt
    20341 tgcacttcac ctgcgcgtgg cgatgccagt caatgttgta gaggctgaca aacagaccgc
    20401 acagaccgag cgtaatcagc caaatcgcgt taagcggaga gatttgcaca tcgtagctta
    20461 ccaacgacag cgcaccgctc acgccaaccg cgccagtcag cacagtgaag cccgcggctg
    20521 ccgtatacag gctacccacc gcgccgccaa ttccggctat ccagccactt agcgcttttt
    20581 gaaaagaaaa gagaaatgcc agaacagcgg cggcgacaaa ccacgccacg ccgctattga
    20641 tcagggaaat tgcgctcatt tagcctctcc actttgagcc tgctgaaaca aggtgagatc
    20701 gccaaagtcc gtgttaaaag tcagctcacg cttacgtttg ctgacgcggg cgatatcggt
    20761 gttatccacc agatgcaggg ctttagtcgg gcacatccgc acgcaggccg gaccttgttc
    20821 atcaaagcta caaaggtcac atttgacggc gatcgcgcga atacctggca cccagtcaag
    20881 caatgtgctg acacgcgccg gagcaggcgg tgccggtggc gctttcgggg tattggtgtt
    20941 tgccggaata tccagcggac ggctgccgga aaattcaatt gcgccaaacg ggcaggcgat
    21001 gccacacagt ttgcagctta cgcacaagct ttcattcagc tgtacggcac catcaacacg
    21061 agtgatggcg ttaaccgggc agaccgttgc gcagggtgca tcttcacagt ggtgacagag
    21121 ctgcggcgca gattcttttt cattgagcat cactcgcagg cgcggcattg attgcaggcc
    21181 gtgctggcga tgcgtctctg aacaggcagc ctcacaagtg tggcagccga tacagagcgt
    21241 ggagtcagca attacaaaac gattcaccag gcattcctca ggtgattgtc atttttgacg
    21301 aaaacatgcc gatgaaatgt cattttcgac actcatcgac acgcccatcc ccaaacaggc
    21361 gtaacgcctg caaaacgggc aaagcctcag ctcatgctac cgggctttgt ccctttacca
    21421 gttggcttaa attcaccggc acattgtttt caatggcggt atataagctg tcgtaaacgc
    21481 cagcgatttt ttcgagatag tgttccagaa cttgttcgcg atcgcggatg ttgaccttgc
    21541 catcaatcat gccgtcaatg ctcaactgcg cctgcgcaat ccgctgtttg tcttcgccat
    21601 cttttgtttc gacgtaatac tcgatggtgt ggctgttaaa gccatccgcc agggtgacgt
    21661 aaatgcgaaa atgttcaaaa aggacgaaac agagttcttt gtccggcgcg cagctcatgg
    21721 cgtgatgcag gcgcgctttc gataacgtga tcccctgaac cagcgaattg cagtagatgt
    21781 gccactggtc ctgtaggcga cgatgccgct gtgcgatgta atcggctttc tcgcttattt
    21841 cccaaatagt cattgtcagg ttacccgttt aacagagatg ccagctttaa gcattttctg
    21901 tgccaacttt taattcattg ttattaaagc gatttctaag ctaaagatga atttcgtcgc
    21961 cgtgtcgacg tgtcatttcg acatcatcga cattattcac cgcagggata atcaacactg
    22021 gcacaattat tgcttgtagc tggcaatagt taatgggagg cgatatgcac gaaataaccc
    22081 tctgccaacg ggcactggaa ttgatcgaac agcaggccgc aaaacacggc gcaaaacgcg
    22141 taactggggt ctggctcaaa attggcgcat tttcttgtgt cgaaaccagc tctcttgcct
    22201 tttgttttga tctggtttgc cgcggcagcg tggcggaagg ttgtaaactg cacctcgaag
    22261 aacaagaggc cgaatgctgg tgtgaaacat gccaacagta tgtgacgcta ctgacccagc
    22321 gcgtccgccg ctgtccacag tgtcatggtg acatgctaca gattgtggca gacgacggtt
    22381 tacagattcg gcggatagaa atagaccagg agtgagcgat gtgtacaaca tgcggttgcg
    22441 gtgaaggcaa cctctatatc gagggtgatg aacataaccc tcattccgcg tttcgtagcg
    22501 cgccatttgc cccggcggca cgcccgaaga tgaaaatcac cggcattaaa gcgcctgaat
    22561 ttacccccag ccagactgaa gaaggcgatc tgcattacgg tcatggcgaa gcgggcactc
    22621 acgcgccggg catgagccag cgtcggatgc tggaagtcga aattgacgtg ctggacaaaa
    22681 ataaccgtct ggctgaacgc aaccgcgcgc gctttgctgc ccgcaagcaa ctggtgctca
    22741 acctggtttc cagccctggt tccggtaaaa ccaccctgct gacggaaact ttaatgcgcc
    22801 tgaaagacag cgttccgtgc gcggttattg aaggtgacca gcaaaccgtg aacgatgccg
    22861 cacgcattcg cgctaccggc accccagcga ttcaggtgaa caccggtaaa ggctgccatc
    22921 ttgacgcaca gatgattgcc gacgccgcgc cgcgcctgcc actggacgat aacggtattc
    22981 tgtttatcga aaacgtcggt aacctcgttt gcccggccag cttcgatctc ggtgaaaaac
    23041 acaaagtggc ggtgctttcc gttaccgaag gtgaagacaa accgctgaaa tatccgcaca
    23101 tgtttgccgc cgcctcgctg atgctgctca acaaagttga cttgctgccg tatctcaact
    23161 ttgacgttga gaagtgcatc gcctgcgccc gtgaagtcaa tccagaaatt gaaatcatcc
    23221 ttatttccgc caccagcggc gaagggatgg accagtggct gaactggctg gagacacagc
    23281 gatgtgcata ggcgttcccg gccagatccg caccattgac ggcaaccagg cgaaagtcga
    23341 cgtctgcggc attcaacgcg atgtcgattt aacgttagtc ggcagctgcg atgaaaacgg
    23401 tcagccacgc gtgggccagt gggtactggt tcacgttggc tttgccatga gcgtaattaa
    23461 tgaagccgaa gcacgcgaca ctctcgacgc cttacaaaac atgtttgacg ttgagccaga
    23521 tgtcggcgcg ctgttgtatg gcgaggaaaa ataatgcgtt ttgttgatga atatcgcgcg
    23581 ccggaacagg taatgcagtt aattgagcat ctgcgcgaac gtgcttcaca tctctcttac
    23641 accgccgaac gccctctgcg gattatggaa gtgtgtggcg gtcataccca cgccattttt
    23701 aaattcggcc tcgaccagtt gctaccggaa aacgttgagt ttatccacgg tccgggttgc
    23761 ccggtgtgcg tactgccaat gggtagaatc gacacctgcg tggagattgc cagccatccg
    23821 gaagtcatct tctgtacctt tggcgatgcc atgcgcgtac cagggaaaca ggggtcgctg
    23881 ttgcaggcaa aagcacgcgg tgccgatgtg cgcatcgttt actcgccaat ggatgcgttg
    23941 aaactggcgc aggagaatcc aacccgtaaa gtggtattct tcggcttagg ttttgaaacc
    24001 accatgccga ccaccgccat cactctgcaa caggcgaaag cccgcgatgt gcagaatttt
    24061 tacttcttct gccagcacat tacgcttatc ccgacactgc gcagtttgct ggaacagcca
    24121 gataacggta tcgacgcgtt cctcgcgccg ggccacgtca gtatggttat cggcactgat
    24181 gcctataatt ttatcgccag cgattttcat cgtccgctag tggtcgctgg tttcgaaccc
    24241 cttgatctac ttcaaggcgt ggtgatgctg gtggagcaga aaatagcggc ccacagcaag
    24301 gtagagaatc agtatcgtcg ggtggtaccg gatgccggta acctgctggc gcaacaggcc
    24361 attgctgatg tgttctgtgt caacggcgac agtgaatggc gcggcttagg cgtgattgaa
    24421 tcttctggcg tgcacctgac gccggattat caacgattcg atgccgaagc acatttccgc
    24481 ccggcaccgc agcaggtctg cgatgacccg cgtgcgcgtt gtggcgaagt cttgacgggc
    24541 aaatgtaagc cgcatcaatg cccgctgttt ggtaacacct gtaatcctca aaccgcgttt
    24601 ggtgcgctga tggtttcctc cgaaggagcg tgcgccgcgt ggtatcagta tcgtcagcag
    24661 gagagtgaag cgtgaataat atccaactcg cccacggcag cggcggccag gcgatgcagc
    24721 aattaatcaa cagcctgttt atggaagcct ttgccaaccc gtggctggca gagcaggaag
    24781 atcaggcccg tcttgatttg gcacagctgg tagcggaagg cgaccgtctg gcgttctcca
    24841 ccgacagtta cgtcattgac ccgctgttct ttcctggcgg taatatcggc aagctggcga
    24901 tttgcggcac cgccaatgac gttgcggtca gtggcgcaat tccgcgctat ctctcctgtg
    24961 gctttatcct cgaagaagga ttgccgatgg agacactgaa agccgtagtg accagcatgg
    25021 cagaaaccgc ccgtgcggca ggcattgcca tcgttactgg cgacaccaaa gtggtacagc
    25081 gcggcgcggc agataaactg tttatcaaca ccgctggcat gggcgcaatt ccggcgaata
    25141 ttcactgggg cgcacagacg ctaaccgcag gcgatgtatt gctggttagc ggtacactcg
    25201 gcgaccatgg ggcgactatc cttaacctgc gtgagcaact agggctggat ggagaactgg
    25261 tcagcgactg cgcggtgctg gcgccgctta ttcagacgct gcgtgacatt cccggcgtga
    25321 aagcgctgcg tgatgccacc cgtggtggtg taaacgcggt ggttcatgag ttcgcggcag
    25381 cctgcggttg tggtattgaa ctttcagaag cggcactgcc ggttaaacct gctgtgcgcg
    25441 gcgtttgcga actgctggga ctggacgccc ttaactttgc caacgaaggc aaactggtga
    25501 tcgccgttga acgcaacgca gcagagcaag tgctggcggc gttacattcc catccattgg
    25561 gaaaagatgc ggcgctgatt ggcgaagtag tagaacgtaa aggtgttcgt cttgccggtc
    25621 tgtatggcgt gaaacgaacc ctcgatctac cgcacgccga accgcttccg cgtatatgct
    25681 aataaaattc taaatctcct atagttagtc aatgaccttt tgcaccgctt tgcggtgctt
    25741 tcctggaaga acaaaatgtc atatacaccg atgagtgatc tcggacagca agggttgttc
    25801 gacatcactc ggacactatt gcagcagccc gatctggcct cgctgtgtga ggctctttcg
    25861 caactggtaa agcgttctgc gctcgccgac aacgcggcga ttgtgttgtg gcaagcgcag
    25921 actcaacgtg cgtcttatta cgcatcgcgt gaaaaagaca cccccattaa atatgaagac
    25981 gaaactgttc tggcacacgg tccggtacgc agcattttgt cgcgccctga tacgctgcat
    26041 tgcagttacg aagaattttg tgaaacctgg ccgcagctgg ccacaggtgg gctatacccc
    26101 aaatttggtc actattgcct gatgccactg gcggcggaag ggcatatttt tggtggctgt
    26161 gaatttattc gttatgacga tcgcccctgg agcgaaaaag agttcaatcg tctgcaaaca
    26221 tttacgcaga tcgtttctgt cgtcaccgaa caaatccaga gccgcgtcgt taacaatgtc
    26281 gactatgagt tgttatgccg ggaacgcgat aacttccgca tcctggtcgc catcactaac
    26341 gcggtgcttt cccgcctgga tatggacgaa ctggtcagcg aagtcgccaa agaaatccac
    26401 tactacttcg acattgacga tatcagcatc gtcttacgca gccaccgtaa gaacaaactc
    26461 aacatctact ccactcacta tcttgataaa cagcatcccg ctcacgaaca gagcgaagtc
    26521 gatgaagccg gaaccctcac cgaacgcgtc ttcaaaagta aagagatgct gttgattaat
    26581 ctccacgagc gggacgattt agccccctat gaacgcatgt tgttcaacac ctggggcaat
    26641 cagattcaaa ccttgtgcct gttaccgctg atgtctggcg acaccatgct gggcgtactg
    26701 aaactggcgc aatgcgaaga gaaagtcttt accactacca atctgaattt actgcgccag
    26761 attgccgaac gtgtggcaat cgctgtcgat aacgccctcg cctatcagga aattcatcgt
    26821 ctgaaagaac ggctggttga tgaaaacctc gccctgaccg agcagctcaa caatgttgat
    26881 agtgaatttg gcgagattat tggccgcagc gaagccatgt acagcgtact taaacaagtt
    26941 gaaatggtgg cgcaaagtga cagtaccgtt ctgatcctcg gtgaaactgg tacaggtaaa
    27001 gagctgattg cccgcgcgat ccataatctc agtgggcgta ataatcgccg catggtcaaa
    27061 atgaactgcg cggcgatgcc tgccggattg ctggaaagcg atctatttgg tcatgagcgc
    27121 ggggctttta ccggtgccag cgctcagcgc attggtcgtt ttgaactggc ggataaaagc
    27181 tcactattcc tcgatgaagt gggcgatatg ccgctggagt tacagccgaa gttgctgcgt
    27241 gtattacagg aacaggagtt tgaacgtctc ggcagcaaca aaatcattca gacggacgta
    27301 cgtttaattg ccgcgactaa ccgcgatctg aaaaaaatgg tcgccgaccg agagttccgt
    27361 agcgatctct attaccgcct gaacgtattc ccgatccacc tgccgccact acgcgagcgt
    27421 ccggaagata ttccgctgct ggcgaaagcc tttaccttca aaattgcccg tcgtctgggg
    27481 cgcaatatcg acagcattcc tgccgagacg ctgcgcatcc tgagcaacat ggaatggccg
    27541 ggcaacgtgc gtgagctgga aaacgtcatc gagcgcgcgg tattgctaac acgcggcaac
    27601 gtgctgcagc tgtcattgcc agatattgct ttaccagaac ctgaaacgcc gcctgccgca
    27661 acggttgtcg cccaggaggg cgaagatgaa tatcagttga ttgttcgcgt gctgaaagaa
    27721 acaaacggcg tggttgccgg gcctaaaggc gctgcgcaac gtctggggct gaaacgcacg
    27781 actctgctgt cacgaatgaa gcgactggga attgataaat cggcattgat ttaacagatg
    27841 tggttatact cgatcaacat tcaatgaaag gaggcagtat ggcagtttca gcaagaaatc
    27901 aattaaccgg aacagttagc gcagtagcaa tgggcgcggt gaatgacgaa gtagaactga
    27961 cgctggcagg tggtgcaaag ctggtggcaa ttgtgactca cagcagccag caagcgctgg
    28021 gtctggcgaa agggaaagaa gcgattgccc tgattaaagc cccgtgggta acgctggcaa
    28081 cggaagactg tggactgaaa ttctctgctc gcaaccagtt tgctggcagc gtgtcgacaa
    28141 tcaccgaagg cgcagtgaac gccactgtcc atatcaaaac ggatgcgggt tttgaaattg
    28201 tggcagtggt aacaaatgaa agtcaggatg aaatgaaact caccactggc tcgcgcgtta
    28261 ttgcattgat caaagcatca gccatcctga ttgccaccaa agcgtaagcg ccgtatttgc
    28321 cggagagtat ggctctccgg cagatttatt tctttggacg atatttttct ggcaactccg
    28381 gcaccggacg cttgtcatcg atgagatgac gcacggttaa gatcggatga cgccacagca
    28441 ttctcggccc ggcccaacgc ataatctgtt tcatctcttc acgctttgca ggctggtaac
    28501 agtgcaccgg acactgctta caggctggtt tctcttcgcc gaacacacat ttatccagcc
    28561 gcttttgcgc gtaagcaaac aacgcctcgt aatgctccgg ctctgctgac gcctgcgggc
    28621 atttcgcttg ataaagatcg atcatttttt taatcgtcag tttttcacga gagatacgct
    28681 tgccggacat gctgcctcca tactcttaag gtgcatttat attacaactt aattttaaag
    28741 gggactatgg ctctgaagga ggaggtttct tcaaccgacg aatttggcga tcttgtttca
    28801 tcgtcatcac gcaaaagctg caaagcagca tttttcgcgg aaccgacatc aagaactcac
    28861 ctttcgcgtc ttcccctgaa atgattaact ccggtatcat gtgcgcctta tgtgattaca
    28921 acgaaaataa aaaccatcac actacattta acatcaggga gccggactta accccatgag
    28981 tacaatagaa aatttcgacg cccatacgcc catgatgcag cagtatctca agctgaaagc
    29041 ccagcatccc gagatcctgc tgttttaccg gatgggcgac ttttatgaac tgttttatga
    29101 cgacgcaaaa cgggcgtcgc aactgctgga tatttcactg accaaacgcg gtgcttcagc
    29161 gggagagcca atcccgatgg cggggattcc ctaccatgcg gtggaaaact acctcgccaa
    29221 actggtgaat cagggcgaat ccgtcgctat ttgtgaacag attggcgatc cggcgaccag
    29281 caaagggccg gtcgagcgca aagttgtccg tatcgttacg ccgggcacca tcagcgatga
    29341 agcgctgttg caggaacgtc aggacaacct gctggcggct atctggcagg acagtaaagg
    29401 tttcggctac gcaacgctgg atatcagttc cggtcgtttt cgcctgagcg aaccggcgga
    29461 ccgcgaaacg atggcggcag aactgcaacg tacgaatcca gcggaattac tgtatgcaga
    29521 agatttcgcc gagatgtcgc tgattgaagg tcgccgcggc ctgcgtcgtc gcccgctgtg
    29581 ggagtttgaa atcgacaccg ctcgccagca attgaacctg caatttggca cccgagatct
    29641 ggtcggtttt ggtgtggaga acgcaccacg cggactttgt gctgccggtt gtctgttgca
    29701 gtatgcgaaa gatacccaac gcacgaccct gccgcatatc cgttctatca ctatggaacg
    29761 tcagcaggac agcatcatta tggatgccgc gacgcgtcgt aacctggaaa ttactcagaa
    29821 cctggccggc ggtgcggaaa atacgctggc ttcagtgctc gactgcactg taacgccgat
    29881 gggtagtcgt atgttgaaac gctggctgca tatgccagtg cgcgataccc gcgtgttgct
    29941 tgagcgtcag caaactattg gcgcattgca ggatttcacc gccgagctgc agccggtact
    30001 gcgtcaggtg ggcgacctgg aacgtattct ggcgcgtctg gcgttgcgta ccgctcgccc
    30061 acgcgatctg gcccgtatgc gtcacgcttt ccagcaactg ccggagttgc gtgcgcagtt
    30121 agaaaatgtc gatagtgcac cggtacaagc gctgcgtgag aagatgggcg agtttgccga
    30181 gctgcgcgat ctgctggagc gagcaatcat cgacacacca ccggtgctgg tacgcgacgg
    30241 tggtgttatc gcatcgggct ataacgaaga gctggatgag tggcgcgcgc tggctgacgg
    30301 cgcgaccgat tatctggagc gtctggaagt ccgcgagcgt gaacgtaccg gcctggacac
    30361 gctgaaagtt ggctttaatg cggtgcacgg ctactacatt caaatcagcc gtgggcaaag
    30421 ccatttggca ccaatcaact acatgcgtcg ccagacgctg aaaaacgccg agcgctacat
    30481 cattccagag ctaaaagagt acgaagataa agttctcacc tcaaaaggca aagcactggc
    30541 tctggaaaaa caactttatg aagagctgtt cgacctgctg ttgccgcatc tggaagcgtt
    30601 gcaacagagc gcgagcgcgc tggcggaact cgacgtgctg gtgaacctgg cggaacgggc
    30661 ctataccctg aactatacct gcccgacctt cattgataaa cctggcattc gtattaccga
    30721 aggtcgccat ccggtagttg aacaagtact gaatgagccg tttatcgcta acccgctgaa
    30781 cctgtcgccg cagcgtcgca tgttgatcat taccggtccg aacatgggcg gtaaaagtac
    30841 ctatatgcgc cagaccgcgt tgattgcgct gatggcgtat atcggcagct acgtaccggc
    30901 gcaaaaagtc gagattggcc cgattgaccg tatctttacc cgcgtaggtg ctgcggatga
    30961 tctggcttcc gggcgttcaa cctttatggt ggagatgacc gaaaccgcca atattttaca
    31021 taacgccacc gaatatagtc tggtgttgat ggatgagatc gggcgtggaa cgtccactta
    31081 cgatggtctg tcgctggcgt gggcgtgtgc ggaaaatctg gcaaataaga tcaaagcgtt
    31141 gacgctgttt gctacccact atttcgagct gacccagtta ccggagaaaa tggaaggcgt
    31201 cgccaacgtg catctcgatg cactggagca cggcgacacc attgccttta tgcatagcgt
    31261 gcaggatggc gcggcgagca aaagctacgg cctggcggtt gcagctctgg cgggtgtgcc
    31321 aaaagaggtt attaagcgcg cacggcaaaa actgcgtgag ctggaaagca tttcgccgaa
    31381 cgccgctgct acgcaagtgg atggtacaca aatgtctttg ctgtccgtac cggaagaaac
    31441 ttcgcctgcg gtcgaggcac tggaaaacct cgacccggat tcactgactc cgcgtcaggc
    31501 gctggaatgg atttatcgct tgaagagtct ggtgtaataa taattcccga tagccttttg
    31561 ctatcgggaa tattaacgac aactgacgaa tcaaataaaa ataccctgta taataggaaa
    31621 acttatttta cagggtaaaa ccatgccatc tacacgctat caaaaaatca atgcccatca
    31681 ctatcgccat atatgggccg ttggtgatat tcatggtgac tatcaattat tacaatcccg
    31741 cttacatcaa ctctcttttt gtcctgaaac cgacttactt atttcggtag gcgataacat
    31801 cgatcgtggg ccggagagtc ttaacgtgct gcgcctgtta aaccagccct ggtttatctc
    31861 cgtcaaaggc aaccacgaag cgatggcgct tgaggcattc gaaactggcg atggaaatat
    31921 gtggcttgcc agcggtggtg actggttttt cgatttaaat gattcagagc aacaagaggc
    31981 aatagatctg ttgctgaaat tccatctcct tccacatatt attgaaatca ctaacgacaa
    32041 cataaaatat gtcatcgcac atgcagatta tccggggaag gagtatcagt ttggcaaaga
    32101 aatagcggag agcgaattac tctggcctgt tgatcgtgtg cagaaatcac ttaatagcga
    32161 attacaaaaa ataaacggcg ccgacttctt tatatttggt catatgatgt ttgataacat
    32221 tcagacgttc gctaaccaga tttatataga taccggatcg ccgaaaagcg ggcggctgtc
    32281 attttataaa ataaagtagt ctcatgcttc ttctgtgaag catgagtaac ccggcgttat
    32341 tgcaggtcat tattcatttt tcgctaccag caaagagaga tccagcttcg ccagcgcgcg
    32401 gctggcactc tccggcagac cgtcgtcagt aatgatctga tcaaactcgc ttaatggtaa
    32461 cgccagccat gtcgccacct gtccatattt cgtcgcatca cagaccaaaa ctcgctggcg
    32521 gctggcactg gcaatcgccc gtttcaccgt gactttatct tctgctggcg tagagatccc
    32581 ccgcacactc catgacgatg cagaaataaa agcctgatca atcatcaggc tacgcagcat
    32641 ggtcgcagcg gcttccccaa cacaggaacg gttttcccga cacactgcgc cgccagtgtg
    32701 aataattgtg caattactgt tgtcgagcaa gtagtccgca ataacgaagt cgttagtaac
    32761 cactgtgagt ggctccatgt gaatcagatg ctgtgcaatc gctaacgtgg tcgttcccgc
    32821 atccagatag atacaacgtc ccggctgaac aagacttgcc gccagcttgc caatagccgc
    32881 tttttgcgtc attgccagcg cagtttttac ctgatgagaa ggttcatgcg ccacgcgtcc
    32941 cggagactgg acgcccccgg acaccagcac aacggctccc tgctgctcca gtttttgtaa
    33001 atcccgacga atggtcatat gtgacacatt cattctgtcc gttagttcag caatactaac
    33061 aatgcctttt tcagctacca tctcaaggat gatttggcga cgctctacgg gtatcaactt
    33121 ttgctccttc ctttgtcctg ctgacattct acgctatttg cctgcgaaac gtgcgcggcg
    33181 caactaacgc ttagttcaca taaaataaca cacaatgtta atttttgtga atcagctcac
    33241 catatcgtta tctttccgcg cttatattca caatatcaaa caaaatatca cttaaattaa
    33301 caaggagagc agatgaaaac gggatcggag tttcatgtcg gtatcgttgg cttagggtca
    33361 atgggaatgg gagcagcact gtcatgtgtc cgcgcaggtc tttctacctg gggcgcagac
    33421 ctgaacagca atgcctgcgc tacgttgaaa gaggctggtg cttgcggggt ttctgataac
    33481 gccgcgacgt ttgccgaaaa actggacgct ctgctggtgc tggtggtcaa tgcgacccag
    33541 gttaaacagg tgctgtttgg tgaaaaaggc gttgcacaac atctgaaacc cggtacggca
    33601 gtaatggttt cttccactat cgccaatgct gatgcgcaag aaattgctac cgctctggct
    33661 ggattcggtc tggaaatgct ggatgcgccg gtttctggtg gtgcggtaaa agccgctaac
    33721 ggtgaaatga ctgtcatggc ctccggtagc gatattgcct ttgaacgact ggcacccgtg
    33781 ctggaagccg ttgccggaaa agtttatcgc atcggagcag aaccgggact gggttcgacc
    33841 gtaaaaatta tccaccagtt gttggcgggc gtacatattg ctgccggagc cgaagcgatg
    33901 gcgcttgcag cccgtgcggg gatcccgctg gatgtgatgt atgacgtcgt gaccaatgcc
    33961 gccggaaatt catggatgtt cgaaaaccgg atgcgtcatg tggtggatgg cgattacacc
    34021 ccgcattcag ccgtcgatat ttttgttaag gatcttggtc tggttgccga taccgccaaa
    34081 gccctgcact tcccgctgcc attggcctca acagcattga atatgttcac cagcgccagt
    34141 aacgcgggtt acgggaaaga agacgatagc gcagttatca agattttctc tggcatcacc
    34201 ctaccgggag cgaaatcatg atcaagattg gcgttatcgc tgatgatttt accggcgcga
    34261 cggatatcgc cagttttctg gtggaaaacg gtctaccaac ggtacaaatt aacggtgttc
    34321 caacaggtaa aatgccggaa gcaatcgacg cactggtgat cagcctgaaa acgcgctcct
    34381 gtccggtggt tgaagccaca cagcaatcgc tggcggctct gagctggttg caacagcaag
    34441 gttgcaaaca gatctatttc aaatactgct ctactttcga cagtacggcg aaagggaata
    34501 tcggcccagt taccgatgca ttaatggatg ctctcgacac accgtttacg gtcttctctc
    34561 cggccctgcc agtcaacgga cgtacggttt atcaggggta tttgttcgta atgaatcaac
    34621 tgctggccga atccggaatg cgccatcacc cggtaaatcc catgaccgac agctatcttc
    34681 cccgtctggt tgaagcgcaa tccacagggc gctgcggcgt cgtttcagca catgttttcg
    34741 aacaaggtgt gaaagccgtt cgtcaggagc tggctcgctt acagcaagag ggctatcgct
    34801 acgcggtgct tgatgcgctg accgaacacc atctggaaat tcagggggaa gccttgcgcg
    34861 atgccccact ggtaacgggc ggttctggtc tggcgattgg cctggcccgg cagtgggcgc
    34921 aagaaaacgg taaccaggct cgcgaagcag ggcgtccgct cgctgggcgc ggcgtagtgc
    34981 tctccggttc atgctctcaa atgaccaacc gccaggtggc acattaccgt caaattgcac
    35041 cagcccgtga ggttgatgtg gcacgctgcc tctcaaccga aactctggcc gcttatgcac
    35101 acgagctggc agagtgggtt ctgggccagg aaagtgtact tgctccactg gtttttgcca
    35161 ccgccagcac tgacgcattg gcagcaattc aacagcaata cggtgcacaa aaagccagtc
    35221 aggcagtaga aactctgttt tctcaactag cggcgcggtt agcagcggaa ggcgtgacac
    35281 gctttattgt cgcaggcggt gagacctccg gcgtagtcac acagagcctg ggcataaaag
    35341 ggtttcatat tggcccaacc atttcccccg gcgtgccgtg ggtaaacgca ctggataagc
    35401 ctgtctcact cgcccttaaa tctggcaact tcggtgatga agcctttttt tcacgagccc
    35461 aaagagagtt tttatcatga gcgatttcgc aaaagtagag cagtctttgc gagaggagat
    35521 gacgcggatt gccagttcat tctttcagcg cggatatgca accggttcgg ctggcaatct
    35581 gtcgctgctt ttacctgacg ggaatttact ggcgacaccg acaggttcat gcctgggcaa
    35641 tctcgatccg cagcggcttt ccaaagtcac cgcggatggc gaatggttaa gtggtgacaa
    35701 cccctcgaaa gaggtgctct ttcatctggc gctgtatcgc aacaatccgc tctgtaaagc
    35761 ggtggtgcat ttgcacagta cgtggtcgac ggcgctttcc tgcctggaag ggctggacag
    35821 caataacgtt attcgtccgt tcacaccata cgtggtgatg cggatgggaa acgtcccgct
    35881 ggtgccctat taccgaccgg gcgataaacg catcgcacag gatctggcgg aactggcagc
    35941 agacaatcag gcttttttac tggcaaatca tggcccagtg gtttgcggtg aaagcctgca
    36001 agaagccgcc aacaatatgg aagagctgga ggaaacggca aagctgattt ttattctcgg
    36061 tgaccgcccg atccgttatc tgaccgcagg tgaaattgcg gaattaagga gttaatgcaa
    36121 tgcctcgttt tgcagctaat ttatccatga tgttcaccga agtgcctttt attgaacgct
    36181 tcgccgcagc gcgaaaagcc gggttcgatg ctgtggagtt tctgtttccc tatgactact
    36241 ccccccagca aatccagcag caactggtgc aaaaccatct gacactggcg ctgttcaata
    36301 ccgctcccgg agatattaat gcgggagaat ggggattatc cgcccttccc ggacgtgagc
    36361 acgaagcacg cgcagacatc gacctggcac tggaatatgc attggcgctc aattgtgaac
    36421 aagtccatgt gatggcaggc gtcgtgcccg ctggcgaaga tgctgaacgg taccgggcag
    36481 tatttattga taatctccgc tacgctgccg accgctttgc gccacacggc aagcgaatat
    36541 tggttgaagc actcagcccc ggcgttaagc ctcattatct cttctccagc cagtatcagg
    36601 cactggctat tgttgaagag gttgcgcgag ataacgtgtt tattcaacta gatactttcc
    36661 atgcgcaaaa agtagatggc aacctgacac atttaatccg caactacgcc gggaaatatg
    36721 cgcacgtaca aattgccgga ctacctgatc gacatgaacc ggacgatgga gaaatcaact
    36781 atccgtggct gttccgcctg ttcgatgaag tgggatatca gggctggatc ggttgtgaat
    36841 acaaacctcg tggcctcacc aaagaagggc ttggctggtt tgacgcctgg cgctaattcg
    36901 ctcaatccct aattacaacg tacccataca tccccccatc tacggatggg gtaggttaac
    36961 tataactttc agacagggtt tcccatgtcc acaattacat tgttatgcat tgcgttagct
    37021 ggcgtaatca tgctgttgct actggtcatc aaggcaaagg tacaaccatt cgttgctctg
    37081 ctcctcgtca gcctgttagt cgcacttgcg gcgggtatac cggcgggcga agtgggtaaa
    37141 gtgatgatcg ccgggatggg cggtgtgctt ggttccgtca ctattattat tggtcttggc
    37201 gctatgctgg ggcgtatgat cgaacactct ggtggtgcag agtcactggc taattatttc
    37261 agtcgcaagt taggtgacaa acgaactatc gctgcgctga ctctggcagc gttcttcctc
    37321 ggcattcccg tcttctttga tgtcggcttt attattcttg cgccaatcat ttacggtttt
    37381 gccaaggttg ccaaaatatc accactcaaa tttggcctgc ctgtcgctgg gatcatgctc
    37441 actgttcacg tggcggtacc gccgcatcca ggccctgtcg ccgcagcggg attactccac
    37501 gcagacatcg gctggctaac catcatcggt attgcgattt ctattcccgt agggattgtt
    37561 ggctactttg cagcgaaaat aatcaataag cgccaatatg cgatgtcggt cgaggtactg
    37621 gaacagatgc aactggctcc ggccagtgag gaaggcgcga caaaattaag cgataaaata
    37681 aatccaccgg gcgtcgcgtt agtcacctcg ctaattgtta ttcctatcgc gattatcatg
    37741 gcgggaacgg tttctgcaac actgatgccg ccttcgcatc ccctgcttgg tacgctacag
    37801 ttgatcggct caccaatggt agctctaatg attgcgctgg tgctggcatt ctggttattg
    37861 gctttacgtc gcggctggag cttacaacac accagcgaca ttatgggctc tgcccttccc
    37921 actgcggcgg tagtgatttt ggttactggt gctggagggg tatttggcaa agtgctggtg
    37981 gaatcgggcg tcggtaaagc ccttgccaac atgttacaaa tgattgacct gcctctgtta
    38041 ccagccgcat ttattatttc actggcgctg cgtgcatcgc aggggtcagc aaccgtagca
    38101 atcctgacaa ccggcgggtt actctcagaa gcggtgatgg gattgaatcc aattcagtgc
    38161 gtattggtga cgctggcagc ctgctttggt gggcttggcg cgtcacatat taatgactca
    38221 gggttctgga ttgtgaccaa atatctgggg ttgtcggtag cagacggtct gaaaacctgg
    38281 acagtgttaa cgaccattct cggttttacc ggattcttaa ttacctggtg cgtatggctc
    38341 gtcatctaat aatccagata agatatgatg tatcgtttaa gtgcctgata agggtgtaat
    38401 gtatgccaat attaacctcg gtaatatctc gctaccgagg ttaattttca ctttgcaata
    38461 taattacagt ctcttgcaag ttgattttat cttagtaata agaaccgacc aaccttattg
    38521 ccatttcgat attagcaggc atagatgcgt cgtagccata catatggcca tatttcggac
    38581 gtaatacttc agttactttt ttaataattg cttcgcgctg ggctcgcgca aaatcagcat
    38641 gattatttgc cgttttattc ataaatttac caaaactttc ttcttttctg actttaattg
    38701 tcgtatcccg aagatcatta agaaactggc gttgaaattt aaaatcatcg tgtgaaccaa
    38761 tattaccatg cccgccaata aaatatttcc agtcaagcat ttcaacctga cgcaaattac
    38821 tgtcatggta aaccaacgta tccgatacgg cgaagcccat catcggcagt tggtcaggat
    38881 tcaggagatc cgggctgtga gctacttttt cctgttttag caaccataca gaatggtcgt
    38941 cggtatgtcc tgctttaaca aatcggtgta attcaatggt ctgcttttca aatttaaact
    39001 ggtcatcttt tttacttaaa acctgagtgg cgacgggtaa gcgactgttc ataaattgca
    39061 ttttttcagc agtggcttta ctggcaataa tacgcagatt cacgccttct ttcttcgcct
    39121 catcgttcca aaatggacta tcgccaatgt gatccacatg gtaatgagaa taaaccagcg
    39181 ctgttacggg taatggagtc acatcacgaa ttgcctgtaa taaatattta ccgcgaccct
    39241 ccggagcatc aaacagcaaa acccctttgt ctccgacata aaacgtagtg gaataaaaat
    39301 agcgttgaac ataataagtt ctctcgccaa ttttttgtaa aatataaggc tgagacatat
    39361 ttctttcaaa aaggttattg agtttatcgc ttggcgatat tattttttcc gctgaaggtt
    39421 caacataact tgcattaact gtagacgaat ttgaagtcga aatttccgtt tgagcaaaaa
//
LOCUS       DAHHTG010000031        39424 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.031, whole genome shotgun sequence.
ACCESSION   DAHHTG010000031 DAHHTG010000000
VERSION     DAHHTG010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 39424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39424
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.031"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            complement(92..379)
                     /locus_tag="NBI74_001594"
     CDS             complement(92..379)
                     /locus_tag="NBI74_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebO family protein"
                     /protein_id="HCD1931953.1"
                     /translation="MNEVVNSGVMNIASLVVSVVVLLIGLILWFFINRASSRTNEQIE
                     LLEALLDQQKRQNALLRRLCEANEPEKADKKTVESQKSVEDEDIIRLVAER"
     gene            complement(454..597)
                     /gene="mgrB"
                     /locus_tag="NBI74_001595"
     CDS             complement(454..597)
                     /gene="mgrB"
                     /locus_tag="NBI74_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP/PhoQ regulator MgrB"
                     /protein_id="HCD1931954.1"
                     /translation="MKKFRWVALVVVVLACLLLWAQVFNMMCDQDVQFFSGICAINQF
                     IPW"
     gene            756..995
                     /locus_tag="NBI74_001596"
     CDS             756..995
                     /locus_tag="NBI74_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1931955.1"
                     /translation="MRFIIRTVMLIALVWIGLLLSGYGVLIGSKENAAGLGLQCTYLT
                     ARGTSTVQYLHTKSGFLGITDCPLLRKSNIVVDNG"
     gene            complement(1139..1930)
                     /gene="kdgR"
                     /locus_tag="NBI74_001597"
     CDS             complement(1139..1930)
                     /gene="kdgR"
                     /locus_tag="NBI74_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator KdgR"
                     /protein_id="HCD1931956.1"
                     /translation="MANADLDKQPDSVSSVLKVFGILQALGEEREIGITELSQRVMMS
                     KSTVYRFLQTMKTLGYVAQEGESEKYSLTLKLFELGARALQNVDLIRSADIQMREISR
                     LTKETIHLGALDEDSIVYIHKIDSMYNLRMYSRIGRRNPLYSTAIGKVLLAWRDRDEV
                     MQILEGVEYKRSTERTITSTEALLPVLDEVREQGYGEDNEEQEEGLRCIAVPVFDRFG
                     VVIAGLSISFPTLRFSEERLQEYVAMLHTAARKISAQMGYHDYPF"
     gene            2107..3480
                     /locus_tag="NBI74_001598"
     CDS             2107..3480
                     /locus_tag="NBI74_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416342.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCD1931957.1"
                     /translation="MPKVQADGLPLPQRYGAILTIVIGISMAVLDGAIANVALPTIAT
                     DLHATPASSIWVVNAYQIAIVISLLSFSFLGDMFGYRRIYKCGLVVFLLSSLFCALSD
                     SLQMLTLARVIQGFGGAALMSVNTALIRLIYPQRFLGRGMGINSFIVAVSSAAGPTIA
                     AAILSIASWKWLFLINVPLGIIALLLAMRFLPPNGSRASKPRFDLPSAVMNALTFGLL
                     ITALSGFAQGQSLTLIGAELVVMVVVGIFFIRRQLSLPVPLLPVDLLRIPLFSLSICT
                     SVCSFCAQMLAMVSLPFYLQTVLGRSEVETGLLLTPWPLATMVMAPLAGYLIERVHAG
                     LLGALGLFIMAAGLFSLVLLPASPADINIIWPMILCGAGFGLFQSPNNHTIITSAPRE
                     RSGGASGMLGTARLLGQSSGAALVALMLNQFGDNGTHVSLIAAAILAVIAACVSGLRI
                     TQPRSRA"
     gene            complement(3526..4407)
                     /gene="htpX"
                     /locus_tag="NBI74_001599"
     CDS             complement(3526..4407)
                     /gene="htpX"
                     /locus_tag="NBI74_001599"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014229823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HCD1931958.1"
                     /translation="MMRIALFLLTNLAVMVVFGLVLSLTGIQSSSVQGLMIMALLFGF
                     GGSFVSLLMSKWMALRSVGGEVIEQPRNERERWLVNTVATQARQAGIAMPQVAIYHAP
                     DINAFATGARRDASLVAVSTGLLQNMSPDEAEAVIAHEISHIANGDMVTMTLIQGVVN
                     TFVIFISRILAQLAAGFMGGNRDEGEESNGNPLIYFAVATVLELVFGILASIITMWFS
                     RHREFHADAGSAKLVGREKMIAALQRLKTSYEPQEATSMMAFCINGKSKSLSELFMTH
                     PPLDKRIEALRTGEYLK"
     gene            complement(4599..6647)
                     /gene="prc"
                     /locus_tag="NBI74_001600"
     CDS             complement(4599..6647)
                     /gene="prc"
                     /locus_tag="NBI74_001600"
                     /EC_number="3.4.21.102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy terminal-processing peptidase"
                     /protein_id="HCD1931959.1"
                     /translation="MNMFFRLTALAGLLAIAGQTFAVEDITRADQIPVLKEETQHATV
                     SERVTSRFTRSHYRQFDLDQAFSAKIFDRYLNLLDYSHNVLLASDVEQFAKKKTELGD
                     ELRSGKLDVFYDLYNLAQKRRFERYQYALSVLEKPMDFTGNDTYNLDRSKAPWPKNEA
                     ELNALWDSKVKFDELSLKLTGKTDKEIRETLTRRYKFAIRRLAQTNSEDVFSLAMTAF
                     AREIDPHTNYLSPRNTEQFNTEMSLSLEGIGAVLQMDDDYTVINSMVAGGPAAKSKAI
                     SVGDKIVGVGQTGKPMVDVIGWRLDDVVALIKGPKGSKVRLEILPAGKGTKTRTVTLT
                     RERIRLEDRAVKMSVKTVGKEKVGVLDIPGFYVGLTDDVKVQLQKLEKQNVSSVIIDL
                     RSNGGGALTEAVSLSGLFIPSGPIVQVRDNNGKVREDSDTDGQVFYKGPLVVLVDRFS
                     ASASEIFAAAMQDYGRALVVGEPTFGKGTVQQYRSLNRIYDQMLRPEWPALGSVQYTI
                     QKFYRVNGGSTQRKGVTPDIIMPTGNEETETGEKFEDNALPWDSIDAATYVKSGDLTA
                     FEPELLKEHNARIAKDPEFQNIMKDIARFNAMKDKRNIVSLNYAVREKENNEDDATRL
                     ARLNERFKREGKPELKKLDDLPKDYQEPDPYLDETVNIALDLAKLEKARPAEQPAPVK
                     "
     gene            complement(6667..7365)
                     /gene="proQ"
                     /locus_tag="NBI74_001601"
     CDS             complement(6667..7365)
                     /gene="proQ"
                     /locus_tag="NBI74_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707288.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone ProQ"
                     /protein_id="HCD1931960.1"
                     /translation="MENQPKLNSSKEVIAFLAERFPHCFSAEGEARPLKIGIFQDLVD
                     RVAGEMNLSKTQLRSALRLYTSSWRYLYGVKSGATRVDLDGNPCGELDEQHVEHARKQ
                     LEEAKARVQAQRAEQQAKKREAAAAAGEKEDAPRRERKPRPTTPRRKEGAERKPRAQK
                     PVEKAPKTVKAPREEQHTPVSDISALTVGQALKVKAGQNAMDATVLEITKDGVRVQLN
                     SGMSLIVRAEHLVF"
     gene            complement(7462..7959)
                     /gene="msrC"
                     /locus_tag="NBI74_001602"
     CDS             complement(7462..7959)
                     /gene="msrC"
                     /locus_tag="NBI74_001602"
                     /EC_number="1.8.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine (R)-S-oxide reductase"
                     /protein_id="HCD1931961.1"
                     /translation="MNKAEFYADLNRDFNALMAGETSFLATLANTSALLYERLTDVNW
                     AGFYLLEDDTLVLGPFQGKIACVRIPVGRGVCGTAVARNQVQRIEDVHAFDGHIACDA
                     ASNSEIVLPLVVKNQIIGVLDIDSTVFGRFTDEDEQGLRQLVAQLEKVLATTDYKKFF
                     ASVAG"
     gene            8089..9372
                     /gene="yebS"
                     /locus_tag="NBI74_001603"
     CDS             8089..9372
                     /gene="yebS"
                     /locus_tag="NBI74_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity lipid transport subunit YebS"
                     /protein_id="HCD1931962.1"
                     /translation="MALNTPQITPTKKITVRAIGEELPRGDYQRCPQCDMLFSLPEIN
                     SHQSAYCPRCQAKIRDGRDWSLTRLAAMAFTMLLLMPFAWGEPLLHIWLLGIRIDANV
                     MQGIWQMTKQGDAITGAMVFFCVIGAPLILVTSIAYLWFGNRLGMNLRPVLLMLERLK
                     EWVMLDIYLVGIGVASIKVQDYAHIQAGVGLFSFVALVILTTVTLSHLNVEELWERFY
                     PQRPATRRDEKLRVCLGCHFTGYPDQRGRCPRCHIPLLLRRRHSLQKCWAALLASIVL
                     LLPANLLPISIIYLNGGRQEDTILSGIMSLASSNIAVAGIVFIASILVPFTKVIVMFT
                     LLLSIHFKCQQGLRTRILLLRMVTWIGRWSMLDLFVISLTMSLINRDQILAFTMGPAA
                     FYFGAAVILTILAVEWLDSRLLWDAHESGNARFDD"
     gene            9341..11974
                     /gene="yebT"
                     /locus_tag="NBI74_001604"
     CDS             9341..11974
                     /gene="yebT"
                     /locus_tag="NBI74_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding membrane homeostasis protein YebT"
                     /protein_id="HCD1931963.1"
                     /translation="MSQETPASTTEAQIKNKRRISPFWLLPFIALMIAGWLIWDSYQD
                     RGNTVTIDFMSADGIVPGRTPVRYQGVEVGTVQDISLSDDLRKIEVKVSIKSDMKDAL
                     REETQFWLVTPKASLAGVSGLDALVGGNYIGMMPGKGKEQDHFVALDTQPKYRLDNGD
                     LMIHLQAPDLGSLSSGSLVYFRKIPVGKVYDYAINPNKQGVVIDVLIERRFTDLVKKG
                     SRFWNVSGVDANVSISGAKVKLESLAALVNGAIAFDSPEESKPAEAEDTFGLYEDLAH
                     SQRGVIIKLELPGGAGLTADSTPLMYQGLEVGQLTKLDLNPGGKVTGEMTVDPSVVTL
                     LRENTRIELRNPKLSLSDANLSALLTGKTFELVPGDGEPRKEFVVVPGEKALLQEPDV
                     LTLTLTAPESYGIDAGQPLILHGVQVGQVIDRKLTSKGVTFTVAIEPQHRELVKGDSK
                     FVVNSRVDVKVGLDGVEFLGASASEWINGGIRILPGDKGEMKASYPLYANLEKALENS
                     LSDLPTTTVSLSAETLPDVQAGSVVLYRKFEVGEVITVRPRANAFDIDLHIKPEYRNL
                     LTSNSVFWAEGGAKVQLNGSGLTVQASPLSRALKGAISFDNLSGASASQRKGDKRILY
                     ASETAARAVGGQITLHAFDAGKLAVGMPIRYLGIDIGQIQTLDLITARNEVQAKAVLY
                     PEYVQTFARGGTRFSVVTPQISAAGVEHLDTILQPYINVEPGRGNPRRDFELQEATIT
                     DSRYLDGLSIIVEAPEAGSLGIGTPVLFRGLEVGTVTGMTLGTLSDRVMIAMRISKRY
                     QHLVRNNSVFWLASGYSLDFGLTGGVVKTGTFNQFIRGGIAFATPPGTPLAPKAQEGK
                     HFLLQESEPKEWREWGTALPK"
     gene            12054..13493
                     /gene="rsmF"
                     /locus_tag="NBI74_001605"
     CDS             12054..13493
                     /gene="rsmF"
                     /locus_tag="NBI74_001605"
                     /EC_number="2.1.1.178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1407)-C(5))-methyltransferase
                     RsmF"
                     /protein_id="HCD1931964.1"
                     /translation="MAQHTVYFPDAFLTQMREAMPSTLSFDDFLAACQRPLRRSIRVN
                     TLKTSVADFLQLTAPYGWTLTPIPWCEEGFWIERDNEDALPLGSTAEHLSGLFYIQEA
                     SSMLPVAALFADGNAPQRVMDVAAAPGSKTTQIAARMNNEGAILANEFSASRVKVLHA
                     NISRCGISNVALTHFDGRVFGAAVPEMFDAILLDAPCSGEGVVRKDPDALKNWSPESN
                     QEIAATQRELIDSAFHALRPGGTLVYSTCTLNREENEAVCLWLKETYPDAVEFLPLGD
                     LFPGANKALTEEGFLHVFPQIYDCEGFFVARLRKTQAIPVLPAPKYKVGNFPFSPVKD
                     REAGQIRQAAASVGLNWDENLRLWQRDKELWLFPVGIEALIGKVRFSRLGIKLAETHN
                     KGYRWQHEAVIALASPDNVNAFELTPQEAEEWYRGRDVYPHAAPVADDVLVTFQHQPI
                     GLAKRIGSRLKNSYPRELVRDGKLFTGNA"
     gene            13610..13846
                     /locus_tag="NBI74_001606"
     CDS             13610..13846
                     /locus_tag="NBI74_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1480 family protein"
                     /protein_id="HCD1931965.1"
                     /translation="MKTSVRIGAFEIDDGELHGESPGDRTLTIPCKSDPDLCMQLDAW
                     DAETSIPALLNGEHSVLYRTRYDQQSDAWIMRLA"
     gene            13951..14142
                     /locus_tag="NBI74_001607"
     CDS             13951..14142
                     /locus_tag="NBI74_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebW family protein"
                     /protein_id="HCD1931966.1"
                     /translation="MFALVLFVCYLDGGCEDIVVDVYNTEQQCLYSMSDQRIRHGGCF
                     PIEDFIDGFWRPAQEYGDF"
     gene            complement(14143..14799)
                     /gene="pphA"
                     /locus_tag="NBI74_001608"
     CDS             complement(14143..14799)
                     /gene="pphA"
                     /locus_tag="NBI74_001608"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase"
                     /protein_id="HCD1931967.1"
                     /translation="MKQPAPVYQRIAGHQWRHIWLSGDIHGCLEQLRRKLWHCRFDPW
                     RDLLISVGDVIDRGPQSLRCLQLLEQHWVRAVRGNHEQMAMDALASRQMSLWLMNGGD
                     WFIALADNQQKQAKTALEKCQHLPFILEVHSRTGKHVIAHADYPDDVYEWQKDVDLHQ
                     VLWSRSRLGERQKGQGIAGADHFWFGHTPLRHRVDIGNLHYIDTGAVFGGELTLVQLQ
                     "
     gene            complement(15194..15535)
                     /locus_tag="NBI74_001609"
     CDS             complement(15194..15535)
                     /locus_tag="NBI74_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebY family protein"
                     /protein_id="HCD1931968.1"
                     /translation="MMKKSILAFLLLTSSAAALAAPQVITVSRFEVGKDKWAFNREEV
                     MLTCRPGNALYVINPSTLVQYPLNDIAQKEVASGKTKAQPISVIQIDDPNNPGEKMSL
                     APFIERAEKLC"
     gene            complement(15548..16420)
                     /gene="copD"
                     /locus_tag="NBI74_001610"
     CDS             complement(15548..16420)
                     /gene="copD"
                     /locus_tag="NBI74_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis membrane protein CopD"
                     /protein_id="HCD1931969.1"
                     /translation="MLAFTWIALRFIHFTSLMLVFGFAMYGAWLAPLTIRRLLTKRFL
                     RLQQHAAVWSLISATAMLAVQGGLMGTGWSDVFSPNIWLAVLQTQFGGVWLWQIVLAL
                     VTLIVALMQPQNMPRLLFMLTTAQFILLAGVGHATLNEGVTAKIHQTNHAIHLICAAA
                     WFGGLLPVLWCMQLIKGRWRHQAIQALMRFSWCGHFAVIGVLASGVLNALLITGFPPT
                     LTTHWGQLLLLKAILVMIMVVIALANRYVLVPRMRQDEDRAAPWFVWMTKLEWAIGAV
                     VLVIISLLATLEPF"
     gene            complement(16424..16798)
                     /gene="yobA"
                     /locus_tag="NBI74_001611"
     CDS             complement(16424..16798)
                     /gene="yobA"
                     /locus_tag="NBI74_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopC domain-containing protein YobA"
                     /protein_id="HCD1931970.1"
                     /translation="MASTARSLRYALAILTTSLVTPSVWAHAHLTHQYPAANAQVTAA
                     PQAITLNFSEGVETGFSGAKITGPKNENIKTLPAKRNEQDQKQLIVPLADSLKPGTYT
                     VDWHVVSVDGHKTKGHYTFSVK"
     gene            16937..17167
                     /gene="holE"
                     /locus_tag="NBI74_001612"
     CDS             16937..17167
                     /gene="holE"
                     /locus_tag="NBI74_001612"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit theta"
                     /protein_id="HCD1931971.1"
                     /translation="MLKNLAKLDQTEMDKVNVDLAAAGVAFKERYNMPVIAEAVEREQ
                     PEHLRSWFRERLIAHRLASVNLSRLPYEPKLK"
     gene            17269..17925
                     /locus_tag="NBI74_001613"
     CDS             17269..17925
                     /locus_tag="NBI74_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HCD1931972.1"
                     /translation="MSFWKVAAAQYEPRKTSLTEQVAHHLEFVRAAARQQCQLLVFPS
                     LSLLGCDYSRRALPAPPDLSLLDPLCYAATTWRMTIIAGLPVEYNDRFIRGIAVFAPW
                     RKTPGIYHQSYGACLGRRSRTITVVDEQPQGMDMDPTCSLFTTGQCLGEPDLLASARR
                     LQFFSHQYSIAVLMANARGNSALWDEHGRLIVRADRGSLLLVGQRSSQGWQGDIIPLR
                     "
     gene            17949..18611
                     /gene="exoX"
                     /locus_tag="NBI74_001614"
     CDS             17949..18611
                     /gene="exoX"
                     /locus_tag="NBI74_001614"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease X"
                     /protein_id="HCD1931973.1"
                     /translation="MLRIIDTETCGLQGGIVEIASVDVIDGKIVNPMSHLVRPDRPIS
                     PQAMAIHRITEAMVADKPWIEDVIPHYYGSEWYVAHNASFDRRVLPEMPGEWICTMKL
                     ARRLWPGIKYSNMALYKTRKLNVQTPPGLHHHRALYDCYITAALLIDIMNTSGWTAEQ
                     MADITGRPSLMTTFTFGKYRGKAVSDVAERDPGYLRWLFNNLDSMSPELRLTLKHYLE
                     NT"
     gene            complement(18608..20668)
                     /gene="ptrB"
                     /locus_tag="NBI74_001615"
     CDS             complement(18608..20668)
                     /gene="ptrB"
                     /locus_tag="NBI74_001615"
                     /EC_number="3.4.21.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase B"
                     /protein_id="HCD1931974.1"
                     /translation="MLPKAARIPHAMTLHGDTRIDNYYWLRDDTRSQPEVLDYLQQEN
                     SYGHRVMASQQALQDRILKEIIDRIPQREVSAPYIKNGYRYRHIYEPGCEYAIYQRQS
                     AFSEEWDEWETLLDANKRAAHSEFYSMGGMAITPDNTIMALAEDFLSRRQYGIRFRNL
                     ETGNWYPELLDNVDPSFVWANDSWTFYYVRKHPVTLLPYQVWRHAIGTPASQDKLIYE
                     EKDDTYYVSLHKTTSKHYVVIHLASATTSEVRLLDAEIADAEPFVFLPRRKDHEYSLD
                     HYQHRFYLRSNRHGKNFGLYRTRMRDEQQWEELIPPRDNIMLEGFTLFTDWLVVEERQ
                     RGLTSLRQINRKTREVIGIAFDDPAYVTWIAYNPEPETARLRYGYSSMTTPDTLFELD
                     MDTGERRVLKQTEVPGFDAANYRSEHLWIVARDGVEVPVSLVYHRKHFRKGHNPLLVY
                     GYGSYGASIDADFSFSRLSLLDRGFVYAIVHVRGGGELGQQWYEDGKFLKKKNTFNDY
                     LDACDALLKLGYGSPSLCYAMGGSAGGMLMGVAINQRPELFHGVIAQVPFVDVVTTML
                     DESIPLTTGEFEEWGNPQDPQYYEYMKSYSPYDNVTAQAYPHLLVTTGLHDSQVQYWE
                     PAKWVAKLRELKTDNHLLLLCTDMDSGHGGKSGRFKSYEGVAMEYAFLVALAQGTLPA
                     TPAD"
     gene            complement(20877..21536)
                     /locus_tag="NBI74_001616"
     CDS             complement(20877..21536)
                     /locus_tag="NBI74_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HCD1931975.1"
                     /translation="MANWLNQLQSLLGQSRSSTSSSADQGLGKLLVPGALGGLAGLLV
                     ANKSARKLLTKYGTNALLVGGGAVAGTVLWNKYKDKIRAAHQDEPQFGAQSTPLDERT
                     ERLILALVFAAKSDGHIDAKERAAIDLQLREAGVEEKGRVLIEQAIEQPLDPQRLATG
                     VRNEEEALEIYFLSCAAIDIDHFMERSYLNALGDALKIPQDVREGIERDLEQQKRTLA
                     E"
     gene            complement(21863..22219)
                     /locus_tag="NBI74_001617"
     CDS             complement(21863..22219)
                     /locus_tag="NBI74_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YebF"
                     /protein_id="HCD1931976.1"
                     /translation="MKKRGAFLGLLLVSACASVFAANNETSKSVTFPKCEGLDAAGIA
                     ASVKRDYQQNRVARWADDQKIVGQADPVAWVSLQDIQGKDDKWSVPLTVRGKSADIHY
                     QVSVDCKAGMAEYQRR"
     gene            complement(22286..22576)
                     /gene="yebG"
                     /locus_tag="NBI74_001618"
     CDS             complement(22286..22576)
                     /gene="yebG"
                     /locus_tag="NBI74_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein YebG"
                     /protein_id="HCD1931977.1"
                     /translation="MAVEVKYVVIREGEEKMSFTSKKEADAYDKMLDTADLLDTWLTN
                     SPVQMEDEQREALSLWLAEQKDVLSTILKTGKLPSPQVVGAESEEEDASHAA"
     gene            22710..23888
                     /gene="purT"
                     /locus_tag="NBI74_001619"
     CDS             22710..23888
                     /gene="purT"
                     /locus_tag="NBI74_001619"
                     /EC_number="2.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent phosphoribosylglycinamide
                     formyltransferase"
                     /protein_id="HCD1931978.1"
                     /translation="MTLLGTALRPAATRVMLLGSGELGKEVAIECQRLGVEVIAVDRY
                     ADAPAMHVAHRSHVINMLDGDALRRVVELEKPHYIVPEIEAIATDMLIQLEEEGLNVV
                     PCARATKLTMNREGIRRLAAEELQLPTSTYRFADSENLFREAVAAIGYPCIVKPVMSS
                     SGKGQTFIRSAEQLAHAWEYAQQGGRAGAGRVIVEGVVKFDFEITLLTVSAVDGVHFC
                     APVGHRQEDGDYRESWQPQQMSPLALERAQEIARKVVLALGGYGLFGVELFVCGDEVI
                     FSEVSPRPHDTGMVTLISQDLSEFALHVRAFLGLPVGGIRQYGPAASAVILPQLTSQN
                     VTFDNVQNAVGADLQIRLFGKPEIDGSRRLGVALATAESVVDAIERAKHAAGQVKVQG
                     "
     gene            complement(23944..24585)
                     /gene="kdgA"
                     /locus_tag="NBI74_001620"
                     /gene_synonym="eda"
     CDS             complement(23944..24585)
                     /gene="kdgA"
                     /locus_tag="NBI74_001620"
                     /gene_synonym="eda"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HCD1931979.1"
                     /translation="MKNWKTSAESILTTGPVVPVIVVKKLEHAVPIAKALVAGGVRVL
                     EVTLRTECAVDAIRAIAKEVPEAIVGAGTVLNPQQLAEVTEAGAQFAISPGLTEPLLK
                     AATEGTIPLIPGISTVSELMLGMDYGLKEFKFFPAEANGGVKALQAIAGPFSQVRFCP
                     TGGISPANYRDYLALKSVLCIGGSWLVPADALEAGDYDRITKLAREAVEGAKL"
     gene            complement(24622..26433)
                     /gene="edd"
                     /locus_tag="NBI74_001621"
     CDS             complement(24622..26433)
                     /gene="edd"
                     /locus_tag="NBI74_001621"
                     /EC_number="4.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydratase"
                     /protein_id="HCD1931980.1"
                     /translation="MNPQLLRVTNRIIERSRETRSAYLARIEQAKTSTVHRSQLACGN
                     LAHGFAACQPEDKASLKSMLRNNIAIITSYNDMLSAHQPYEHYPEIIRKALHEANAVG
                     QVAGGVPAMCDGVTQGQDGMELSLLSREVIAMSAAVGLSHNMFDGALFLGVCDKIVPG
                     LTMAALSFGHLPAVFVPSGPMASGLPNKEKVRIRQLYAEGKVDRMALLESEAASYHAP
                     GTCTFYGTANTNQMVVEFMGMQLPGSSFVHPDSPLRDALTAAAARQVTRMTGNGNEWM
                     PIGKMIDEKVVVNGIVALLATGGSTNHTMHLVAMARAAGIQINWDDFSDLSDVVPLMA
                     RLYPNGPADINHFQAAGGVPVLVRELLKAGLLHEDVNTVAGFGLSRYTLEPWLNNGEL
                     DWREGAEKSLDNNVIASFEQPFSHHGGTKVLSGNLGRAVMKTSAVPVENQVIEAPAVV
                     FESQHDVMPAFEAGLLDRDCVVVVRHQGPKANGMPELHKLMPPLGVLLDRCFKIALVT
                     DGRLSGASGKVPSAIHVTPEAYDGGLLAKVRDGDIIRVNGQTGELTLLVDEAELAARE
                     PHIPDLSASRVGTGRELFSALREKLSGAEQGATCITF"
     gene            complement(26668..28143)
                     /gene="zwf"
                     /locus_tag="NBI74_001622"
     CDS             complement(26668..28143)
                     /gene="zwf"
                     /locus_tag="NBI74_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HCD1931981.1"
                     /translation="MAVTQTAQACDLVIFGAKGDLARRKLLPSLYQLEKAGQLNPDTR
                     IIGVGRADWDKAAYTKVVREALETFMKETIDEGLWDTLSARLDFCNLDVNDTAAFSRL
                     GAMLDQKNRITINYFAMPPSTFGAICKGLGEAKLNAKPARVVMEKPLGTSLATSQEIN
                     DQVGEYFEECQVYRIDHYLGKETVLNLLALRFANSLFVNNWDNRTIDHVEITVAEEVG
                     IEGRWGYFDKAGQMRDMIQNHLLQILCMIAMSPPSDLSADSIRDEKVKVLKSLRRIDR
                     SNVREKTVRGQYTAGFAQGKKVPGYLEEEGANKSSNTETFVAIRVDIDNWRWAGVPFY
                     LRTGKRLPTKCSEVVVYFKTPELNLFKESWQDLPQNKLTIRLQPDEGVDIQVLNKVPG
                     LDHKHNLQITKLDLSYSETFNQTHLADAYERLLLETMRGIQALFVRRDEVEEAWKWVD
                     SITEAWAMDNDAPKPYQAGTWGPVASVAMITRDGRSWNEFE"
     gene            28481..29350
                     /gene="hexR"
                     /locus_tag="NBI74_001623"
                     /gene_synonym="yebK"
     CDS             28481..29350
                     /gene="hexR"
                     /locus_tag="NBI74_001623"
                     /gene_synonym="yebK"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HexR"
                     /protein_id="HCD1931982.1"
                     /translation="MNMLEKIQSQLEHLSKSERKVAEVILASPDNAIHSSIAALALEA
                     NVSEPTVNRFCRSMDTRGFPDFKLHLAQSLANGTPYVNRNVNEDDSVESYTGKIFESA
                     MATLDHVRHSLDKSAINRAVDLLTQAKKIAFFGLGSSAAVAHDAMNKFFRFNVPVVYS
                     DDIVLQRMSCMNCSDGDVVVLISHTGRTKNLVELAQLARENDAMVIALTSAGTPLARE
                     ATLAITLDVPEDTDIYMPMVSRLAQLTVIDVLATGFTLRRGTKFRDNLKRVKEALKES
                     RFDKQLLNLSDDR"
     gene            29478..30920
                     /gene="pyk"
                     /locus_tag="NBI74_001624"
     CDS             29478..30920
                     /gene="pyk"
                     /locus_tag="NBI74_001624"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HCD1931983.1"
                     /translation="MSRRLRRTKIVTTLGPATDRDNNLEKVIAAGANVVRMNFSHGSP
                     EDHKMRADKVREIAAKLGRHVAILGDLQGPKIRVSTFKEGKVFLNIGDKFLLDANLGK
                     GEGDKEKVGIDYKGLPADVVPGDILLLDDGRVQLKVLEVQGMKVFTEVTVGGPLSNNK
                     GINKLGGGLSAEALTEKDKADIKTAALIGVDYLAVSFPRCGEDLNYARRLARDAGCDA
                     KIVAKVERAEAVCSQEAMDDIILASDVVMVARGDLGVEIGDPELVGIQKALIRRARQL
                     NRAVITATQMMESMITNPMPTRAEVMDVANAVLDGTDAVMLSAETAAGQYPSETVAAM
                     ARVCLGAEKIPSINVSKHRLDVQFDNVEEAIAMSAMYAANHLKGVTAIITMTESGRTA
                     LMTSRISSGLPIFAMSRHERTLNLTALYRGVTPVHFDSANDGVAAASEAVNLLRDKGY
                     LMSGDLVIVTQGDVMSTVGSTNTTRILTVE"
     gene            complement(31052..32023)
                     /gene="lpxM"
                     /locus_tag="NBI74_001625"
                     /gene_synonym="msbB"
     CDS             complement(31052..32023)
                     /gene="lpxM"
                     /locus_tag="NBI74_001625"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416369.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HCD1931984.1"
                     /translation="METKKNNSEYIPEFDKSFRHPRYWGAWLGVAAMAGIALTPPKFR
                     DPILARLGRFAGRLGKSSRRRALINLSLCFPERSEAEREAIVDEMFATAPQAMVMMAE
                     LAIRGPEKIQPRVDWQGLEIIEEMRRNNEKVIFLVPHGWAVDIPAMLMASQGQKMAAM
                     FHNQGNPVFDYVWNTVRRRFGGRLHARNDGIKPFIQSVRQGYWGYYLPDQDHGPEHSE
                     FVDFFATYKATLPAIGRLMKVCRARVVPLFPIYDGKTHRLTIQVRPPMDDLLEADDHT
                     IARRMNEEVEIFVGPRPEQYTWILKLLKTRKPGEIQPYKRKDLYPIK"
     gene            complement(32142..33464)
                     /gene="mepM"
                     /locus_tag="NBI74_001626"
     CDS             complement(32142..33464)
                     /gene="mepM"
                     /locus_tag="NBI74_001626"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein DD-endopeptidase MepM"
                     /protein_id="HCD1931985.1"
                     /translation="MQQIARSVALAFNNLPRPHRVMLGSLTVLTLAVAVWRPYVYHRD
                     ATPIVKTIELEQNEIRSLLPEASEPIDQAAQEDEAIPQDELDDKIAGEAGVHEYVVST
                     GDTLSSILNQYGIDMGDITQLAAADKELRNLKIGQQLSWTLTADGELQRLTWEVSRRE
                     TRTYDRTAANGFKMTSEMQQGEWVNNLLKGTVGGSFVASARNAGLTSAEVSAVIKAMQ
                     WQMDFRKLKKGDEFAVLMSREMLDGKREQSQLLGVRLRSEGKDYYAIRAEDGKFYDRN
                     GTGLAKGFLRFPTAKQFRISSNFNPRRTNPVTGRVAPHRGVDFAMPQGTPVLSVGDGE
                     VVVAKRSGAAGYYVAIRHGRSYTTRYMHLRKILVKPGQKVKRGDRIALSGNTGRSTGP
                     HLHYEVWINQQAVNPLTAKLPRTEGLTGSDRREFLAQAKEIVPQLRFD"
     gene            complement(33480..34412)
                     /gene="znuA"
                     /locus_tag="NBI74_001627"
     CDS             complement(33480..34412)
                     /gene="znuA"
                     /locus_tag="NBI74_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     ZnuA"
                     /protein_id="HCD1931986.1"
                     /translation="MLHKKTLLFAALSAALWGGATQAADAAVVASLKPVGFIASAIAD
                     GVTETQVLLPDGASEHDYSLRPSDVKRLQNADLVVWVGPEMEAFMQKPVSKLPEAKQV
                     TIAQLEDVKPLLMKSIHDDDDDHDHAEKSDEDHHHGDFNMHLWLSPEIARATAVAIHG
                     KLVELMPQSRAKLDANLKDFEAQLASTETQVGNELAPLKGKGYFVFHDAYGYFEKQFG
                     LTPLGHFTVNPEIQPGAQRLHEIRTQLVEQKATCVFAEPQFRPAVVESVARGTSVRMG
                     TLDPLGTNIKLGKTSYSEFLNQLANQYASCLKGD"
     gene            34491..35246
                     /gene="znuC"
                     /locus_tag="NBI74_001628"
     CDS             34491..35246
                     /gene="znuC"
                     /locus_tag="NBI74_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter ATP-binding protein ZnuC"
                     /protein_id="HCD1931987.1"
                     /translation="MTSLVSLENVSVSFGQRRVLSDVSLELKPGKILTLLGPNGAGKS
                     TLVRVVLGLVTPDEGVIKRNGKLRIGYVPQKLYLDTTLPLTVNRFLRLRPGTHKEDIL
                     PALKRVQAGHLINAPMQKLSGGETQRVLLARALLNRPQLLVLDEPTQGVDVNGQVALY
                     DLIDQLRRELDCGVLMVSHDLHLVMAKTDEVLCLNHHICCSGTPEVVSLHPEFISMFG
                     PRGAEQLGIYRHHHNHRHDLQGRIVLRRGNDRS"
     gene            35243..36028
                     /gene="znuB"
                     /locus_tag="NBI74_001629"
     CDS             35243..36028
                     /gene="znuB"
                     /locus_tag="NBI74_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter permease subunit ZnuB"
                     /protein_id="HCD1931988.1"
                     /translation="MIELLFPGWLAGIMLACAAGPLGSFVVWRRMSYFGDTLAHASLL
                     GVAFGLLLDVNPFYAVIAVTLLLAGGLVWLEKRPQLAIDTLLGIMAHSALSLGLVVVS
                     LMSNIRVDLMAYLFGDLLAVTPEDLISIAIGVVIVVAILFWQWRNLLSMTISPDLAFV
                     DGVKLQRVKLLLMLVTALTIGVAMKFVGALIITSLLIIPAATARRFARTPEQMAGVAV
                     LVGMVAVTGGLTFSAFYDTPAGPSVVLCAALLFIISMMKKQAS"
     gene            complement(36222..37570)
                     /locus_tag="NBI74_001630"
     CDS             complement(join(36222..37090,37090..37570))
                     /locus_tag="NBI74_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558234.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HCD1931989.1"
                     /translation="MKHSFEVKLAAVNHYLAGHAGIISTAKLFQLSHTSLSHWINLFL
                     LHGPRALDCRHKRSYSPEDKLCVVLYALGHSESLPRVAARFNIPSHNTVKNWIKGYRK
                     SGNEAFIRRRKEKSMTRSDDTHENEANMTPEEMKNELRYLRAENAYLKAMQEHLLEKK
                     PPGAGEKTKVIQSLRYGHCQSDLLKAAGLARSTLYYQLSLQKAKDKYADVKQLIASIF
                     HEHRGCYGYRRIHCELQKRGLKFSGKTVRKLMQQLGLKSPVRLKKYRSYRGNMGLAAE
                     NILQRLFKAAAPCEKWVTDITEFRAGGQKLYLSPILDLFNGEIVAWETACRPTEELVK
                     RMLNKGLESLAEGEKPLLHSDQGWHYRIKSYQSALADRGLVQSMSRKGNCLDNAVMEN
                     FFGHLKEEMYYRRDYRNVEELENAVNEYITYWNQKRIKLSLGGLSPVEYRTEYQKAG"
     gene            complement(37680..38690)
                     /gene="ruvB"
                     /locus_tag="NBI74_001631"
     CDS             complement(37680..38690)
                     /gene="ruvB"
                     /locus_tag="NBI74_001631"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HCD1931990.1"
                     /translation="MIEADRLISAGTTLPEDVADRAIRPKLLEEYVGQPQVRSQMEIF
                     IKAAKLRGDALDHLLIFGPPGLGKTTLANIVANEMGVNLRTTSGPVLEKAGDLAAMLT
                     NLEPHDVLFIDEIHRLSPVVEEVLYPAMEDYQLDIMIGEGPAARSIKIDLPPFTLIGA
                     TTRAGSLTSPLRDRFGIVQRLEFYQVPDLQYIVSRSARFMGLEMSDDGALEVARRARG
                     TPRIANRLLRRVRDFAEVKHDGTISADIAAQALDMLNVDAEGFDYMDRKLLLAVIDKF
                     FGGPVGLDNLAAAIGEERETIEDVLEPYLIQQGFLQRTPRGRMATTRAWNHFGITPPE
                     MS"
     gene            complement(38699..39310)
                     /gene="ruvA"
                     /locus_tag="NBI74_001632"
     CDS             complement(38699..39310)
                     /gene="ruvA"
                     /locus_tag="NBI74_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012131997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HCD1931991.1"
                     /translation="MIGRLRGIIIEKQPPLVLIEVGGVGYEVHMPMTCFYELPEAGQE
                     AIVFTHFVVREDAQLLYGFNNKQERTLFKELIKTNGVGPKLALAILSGMSAQQFVNAV
                     EREEVGALVKLPGIGKKTAERLIVEMKDRFKGLHGDLFTPAADLVLTSPASPATDDAE
                     QEAVAALVALGYKPQEASRMVSKIARPDASSETLIREALRAAL"
BASE COUNT         9431 a        10166 c         9663 g        10164 t
ORIGIN      
        1 taatttgaag cagttgcacc atgacagtgc gtataatcag catgaattga gtaacccttc
       61 aacgacgggg ttactcaaga ttgaggaagg attatcgttc ggcgaccaga cgaataatat
      121 cttcgtcttc aaccgatttt tgactctcaa cggtcttttt atctgctttc tcaggttcgt
      181 ttgcttcgca caaacgacgt aacagtgcat tttgacgttt ttgctgatcc agcaacgcct
      241 caagcagttc aatctgttcg ttagtccgtg aactggcacg attgataaaa aaccacaaga
      301 tgagcccgat aagaagaacc accaccgata caaccaaaga cgcaatgttc atcacgcctg
      361 aatttacaac ttcgttcatt ttaccacctc tgagtagagg cgctattcta ccactgctgg
      421 agaggaagaa aatccagtgc tgaaaaaatg atatcaccac gggataaact ggttaatggc
      481 acaaattccg ctgaaaaatt gtacatcctg atcgcacatc atgttgaata cctgcgccca
      541 aagaagcaag caagccaaca ccacgacaac cagagcgacc catcgaaact ttttcactcc
      601 aatctccgtt tcacctacct tatgtcatta aactagcatg cgttagttaa acagcgccta
      661 actatgtcga tattcgtgct ttttcggaat gagtcgcttg cttcagcgca tattgccgct
      721 acgattaagc gaacataaaa agagaagagg ttgtaatgcg attcatcatt cgcacagtta
      781 tgctgatagc actggtatgg ataggattat tactcagcgg atatggcgtt ttgattggaa
      841 gcaaagagaa tgctgcagga ttaggtttgc aatgtaccta tctgactgcc agaggcacca
      901 gtacggtaca atacttgcac actaaaagtg gttttttggg aataacagat tgcccgctgt
      961 tacgcaaaag caatatcgtg gtcgataatg gttaagagta ataaataacc agattctcac
     1021 tcatgagtaa aatattctta tagcaagcgc gtttaatata acgccgccct caactttatt
     1081 tttctcatgc taaataaaga acctgtctgt ttgatattca gacaggttct tactctcatc
     1141 agaacggata gtcgtgataa cccatttggg cagaaatttt gcgcgccgcg gtatgcaaca
     1201 ttgcgacata ttcctgtaaa cgctcttcag agaaacgtaa cgtcgggaag gagatgctca
     1261 aaccggcaat gaccacgcca aagcgatcaa ataccggaac cgcaatgcat cgcagccctt
     1321 cttcctgctc ttcattatct tcgccatacc cctgctcgcg cacctcgtcc agaacgggta
     1381 ataacgcttc tgtactggtg atggtccgct cggtactgcg tttatactcc acgccctcaa
     1441 gaatttgcat cacttcatcg cgatcgcgcc atgccagcag taccttacca atcgcggtgc
     1501 tgtacagcgg attacgacgc ccaatccgtg aatacatgcg caaattgtac attgagtcaa
     1561 ttttgtggat gtaaacaata ctgtcttcgt ccagtgcgcc gaggtggata gtttctttgg
     1621 tcaggcggga gatctcacgc atctggatat ctgcgctacg aattaaatcg acgttttgca
     1681 acgcgcgagc gcccagttca aacaatttca gggtcagcga atatttctcc gactcccctt
     1741 cctgcgccac ataacctaag gttttcatgg tctgtaaaaa gcgataaacg gtgctttttg
     1801 acatcatgac gcgctgcgac aactcggtta tccctatttc gcgctcttca cccagcgcct
     1861 gcaaaatgcc aaaaactttt agcacggaag atacagaatc aggctgttta tccagatctg
     1921 cgttagccat ttatcacctc attgcgagtg ttttataaaa atcagaactg ttttttatta
     1981 taatttcgca cctgggtggt cgcaatccat cttttgccgg ttagttacaa ttctgcgaca
     2041 tccaccgtga atatcagtgc tagaatcata cccctataga ttattcacca aagatataaa
     2101 attcctatgc caaaagttca ggccgacggc ctgccattgc cccagcgata cggtgcgata
     2161 ttaaccattg tgattggtat ttcgatggct gtccttgacg gcgcaatcgc caacgtcgcc
     2221 ttgccaacaa tcgctacgga ccttcatgcc acgccggcca gttccatctg ggtagtgaac
     2281 gcctatcaaa tcgccattgt catctccctg ctctcgtttt cgtttctggg cgatatgttt
     2341 ggctatcgac gtatttataa atgcggtctg gtcgtttttc tgttgtcttc actgttctgc
     2401 gccctttctg attcgctgca aatgctcacc cttgcgcgtg tcatacaagg tttcggcggt
     2461 gcagcgttga tgagcgttaa taccgcactt atccgcctga tctatccaca acgttttctg
     2521 ggtagaggga tgggcataaa ctcgtttatt gttgccgtct cttctgctgc cgggccgaca
     2581 attgctgcag caatcctctc catcgcatcc tggaaatggt tatttttaat caacgtaccg
     2641 ttgggtatta tcgccctgct tctggcgatg cgttttctgc cacccaatgg ttctcgcgcc
     2701 agtaaacccc gtttcgacct gcccagcgcc gtgatgaacg cgttaacctt cggcctgctt
     2761 attactgcat tgagtggttt cgctcagggg caatcgctga cattgattgg tgcggaactg
     2821 gtggtaatgg ttgtcgttgg tattttcttt attcgccgcc agctttctct tcccgtaccg
     2881 ctgctaccgg tggatttact gcgtatcccg ctgttttcac tttctatttg cacatctgtt
     2941 tgctctttct gcgcacaaat gctggcaatg gtttcccttc ccttttacct gcaaaccgtg
     3001 ctcgggcgta gtgaagtcga aacaggttta cttctgacac cgtggccgtt agcaacgatg
     3061 gttatggctc cactggcagg ctatttgatt gaacgcgtac atgcaggatt gctgggtgct
     3121 ttagggttat tcatcatggc tgcggggctt ttttccctgg ttctgctgcc agcgtcacct
     3181 gcggatatca atattatctg gccgatgatc ttatgtggtg ctggatttgg cttgttccag
     3241 tcacccaata accacaccat tattacctcc gctcctcgcg aacgtagcgg tggagccagt
     3301 ggcatgttag ggacggctcg tcttctgggt cagagtagcg gcgcggctct ggtagcgctg
     3361 atgctaaatc agtttggtga taatggtacg cacgtctcgc tgatagctgc ggctattctg
     3421 gcagtgattg ctgcctgtgt cagtggttta cgtatcactc agccacgatc cagggcataa
     3481 aaaaagcgcg tcgatcagga cgcgcttttt agtatttacg tcatattact tcaggtattc
     3541 acccgtacgc agagcttcaa tacgtttatc cagcggcggg tgggtcatga acaactcact
     3601 gagcgatttc gacttaccgt taatgcagaa agccatcatg ctggttgctt cttgcggttc
     3661 atagctggtt ttcaggcgtt gcagcgcggc aatcattttc tcgcgaccaa ccagttttgc
     3721 tgaacccgca tcagcatgga attcacgatg acgcgagaac cacatggtga taatgctcgc
     3781 cagaatacca aacaccagtt ccagaaccgt tgcaaccgca aagtagatca gcgggttgcc
     3841 gttgctctct tcaccttcat cacggtttcc gcccataaaa cccgcggcaa gctgcgccag
     3901 aatacgggaa ataaagataa cgaaggtgtt caccacgccc tgaatcagcg tcatggtgac
     3961 catatcacca ttggcgatgt ggctgatttc gtgagcaatt accgcctcgg cttcatccgg
     4021 gctcatgttc tgcagcaaac cagtgctgac agcaaccaga gaggcatcac ggcgcgcacc
     4081 agttgcaaaa gcattgatgt ccggcgcatg gtagatagcc acttgcggca tagcgatccc
     4141 cgcctgacga gcctgggttg ctacagtatt gaccagccaa cgttcccttt cgttacgcgg
     4201 ttgctcgatc acttccccgc caacagatcg taatgccatc catttggaca tcagaagcga
     4261 aacgaaggaa ccaccaaaac cgaacagcaa ggccatgatc atcagcccct gaacgctgct
     4321 cgactgtatc cctgtcaggc tcagtaccag cccgaaaacg accattacgg ccaggttcgt
     4381 tagcaggaag agcgcgattc gcatcataat tttcttttta cctcagttta acaaaacgca
     4441 atatgcgata cccacatcgt atgggttacg cgactatttt caagtctgga tagtgcgtaa
     4501 gtcaccagaa agacacaact ttacattttg tagcatctga tttacggcat cttgccgctg
     4561 ttaaaaaatc aggcacaatt tcttgtgcct gatagatatt acttgacggg agcgggttgt
     4621 tccgcgggtc tggctttttc aagcttcgcc agatcgagtg cgatattcac cgtctcatcc
     4681 agataaggat ctggctcctg gtaatctttc ggtagatcat ccagtttctt caactccggt
     4741 ttaccttcgc gtttaaagcg ttcgttcaaa cgcgccagac gcgtcgcatc atcttcatta
     4801 ttctctttct cacgcacagc gtaattcaga gaaacgatat tgcgcttgtc cttcatagcg
     4861 ttgaagcgcg cgatatcctt catgatgttc tggaactcag gatctttcgc gatacgcgca
     4921 ttatgttcct tcagcagctc cggttcaaag gccgttaaat ctcctgattt cacataagtc
     4981 gcggcatcaa tgctatccca cggcagcgcg ttatcttcga atttctcacc cgtttccgtt
     5041 tcttcattac ccgtcggcat gatgatgtct ggcgttacgc ctttacgttg cgtactgccg
     5101 ccgttaacgc gatagaactt ctggatcgtg tactgcacag aacccagcgc tggccattca
     5161 ggacgtaaca tctgatcgta aatacggttc aatgaacggt actgctgaac ggtgccttta
     5221 ccaaacgtcg gttcacccac aaccagggca cgaccgtaat cctgcattgc cgcggcaaag
     5281 atttctgaag ccgaagcact gaagcggtca accagcacca ccagcgggcc tttatagaag
     5341 acctgtccgt cggtgtcgct gtcttcacga accttgccgt tgttatcgcg gacctgaaca
     5401 atcgggccgg atggaatgaa cagaccggag agcgatacgg cttcagtcaa cgccccacca
     5461 ccattgctac gcaggtcgat aatcacactg ctgacattct gtttttccag tttctgcagt
     5521 tgcactttga catcgtctgt caaacccaca tagaagcctg gaatatccag cacgccgact
     5581 ttctctttac cgacggtctt caccgacatt ttaaccgcgc ggtcttcgag acgaatacgt
     5641 tcacgggtca acgttacagt acgcgtcttg gtccctttac cagcaggtaa aatttccaga
     5701 cgaactttac tgcccttagg ccctttaatt aaggccacca catcatcaag acgccagcca
     5761 atcacgtcaa ccatcggctt gcctgtttga ccaacaccga caattttgtc accaacgctg
     5821 atagctttgc tcttcgctgc cggacctcct gccaccatcg aattgataac ggtgtagtca
     5881 tcatccattt gcagcactgc gccaatacct tccagcgaca aactcatttc agtgttgaac
     5941 tgttcggtat tacgcgggga aagatagttg gtatgcgggt cgatttcacg tgcaaacgcc
     6001 gtcattgcca gcgagaaaac atcttcgctg ttggtttgcg ccagacgacg aatggcaaat
     6061 ttgtagcggc gagtcagggt ttcacgaatt tccttatccg tttttcctgt cagcttcagg
     6121 cttaactcgt cgaatttgac tttactgtcc cacagcgcgt tcaactcagc ctcgtttttc
     6181 ggccagggcg ctttgctgcg gtcaaggtta taagtgtcgt tgccggtgaa atccatcggc
     6241 ttttccagta ccgacaaagc gtactggtaa cgttcaaaac ggcgcttttg cgccagattg
     6301 tagagatcgt agaaaacgtc gagtttacct gaacgcagtt catcgcctaa ctcagttttc
     6361 tttttcgcga actgttcaac atcgcttgcc agcagcacat tatggctgta atcgagcaga
     6421 ttcaggtagc ggtcaaagat tttggccgaa aatgcctgat cgaggtcgaa ctggcgataa
     6481 tgagaacggg tgaagcgcga cgttacgcgc tcactcaccg tcgcatgctg cgtctcttcc
     6541 tttaataccg gaatttgatc agcacgcgtg atatcttcta cagcgaaggt ctggcctgct
     6601 attgcaagca ggccagctaa cgcggtaagc ctaaaaaaca tgttcatgcc tggcccggcc
     6661 tccgtttcag aacaccaggt gttctgcgcg cacaatcaaa gacatacccg aattcagctg
     6721 gacgcggacg ccgtctttgg tgatttctaa tacggtggca tccatcgcgt tttgacccgc
     6781 tttcaccttc agggcttgtc cgacagtcag agctgaaatg tcagaaaccg gggtgtgctg
     6841 ttcttcgcga ggtgctttta ctgtttttgg cgctttctct accggctttt gcgcacgagg
     6901 tttacgttca gcgccttctt tgcggcgtgg cgtggtcggg cgtggcttgc gttcgcggcg
     6961 cggtgcgtct tctttctcac cagcagctgc ggcagcttcg cgttttttcg cttgctgttc
     7021 agcacgctgt gcctgaacac gcgctttcgc ttcttcaagc tgcttgcgag catgctcgac
     7081 atgttgctcg tccagctcac cgcatgggtt gccgtcaaga tcaacacgcg ttgcgccgga
     7141 tttaacaccg taaagataac gccagctcga agtgtagaga cgtaaagcgg atcgcaattg
     7201 cgttttgctc aggttcattt ccccggcaac acgatcgacc aaatcctgaa aaataccgat
     7261 tttcagcgga cgcgcttcac cttccgcact gaaacagtgg ggaaaacgtt cggccagaaa
     7321 cgcgattact tctttactgc tattcaactt aggttgattt tccatgaaat ttcctgatta
     7381 caacggacgt agccaacaag cgcaggcatg aacaggcgtc attataatga cgctatcagt
     7441 aaatgctacg ttatccgttg attatcctgc gacgctcgca aagaattttt tgtaatccgt
     7501 cgttgcaagc actttttcaa gctgtgccac aagctgacgt aagccttgct cgtcctcgtc
     7561 tgtaaagcga ccgaagacgg tactatcgat gtcgagaaca ccaataatct gatttttcac
     7621 caccagtggc agaacaattt cagaattact cgccgcatca caggcaatat gcccgtcaaa
     7681 cgcgtgaaca tcctcgatac gctgcacttg attgcgggca accgcagtgc cgcatacgcc
     7741 gcgcccaacg ggtatccgga cacaggcaat tttgccctga aatggtccga gcaccagtgt
     7801 atcgtcctca agcaaataaa aacctgccca gtttacgtca gtgagacgct catataacaa
     7861 cgcactggtg ttcgcaagcg ttgccagaaa actggtttct cccgccatca gcgcgttaaa
     7921 gtcgcgattt aaatccgcgt aaaattctgc tttgttcatt atataatcac ttggttgtct
     7981 tacctggaac tgccagccta ttaaaataag cattaaatgc gttaatgctc aagatcattc
     8041 ccatcatggg ttaagattaa tgttaattct tattacattt ggcacgtcat ggctcttaac
     8101 acaccacaaa ttacgccgac aaagaagata acagtgaggg ctatcggcga ggaactgccg
     8161 cgtggtgatt accaacgttg cccgcaatgt gacatgctgt ttagcctgcc cgagataaat
     8221 tctcatcaaa gtgcctattg tccgcgctgt caggcaaaaa ttcgtgacgg gcgcgactgg
     8281 tcgctaacgc gcctggcggc aatggccttc accatgctgt tgcttatgcc gtttgcctgg
     8341 ggcgaaccgc tgttgcatat ctggctgtta ggcattcgta ttgacgccaa cgttatgcaa
     8401 ggcatctggc aaatgaccaa acagggcgat gcgataacgg gggcgatggt ctttttctgc
     8461 gttatcggtg cccccctcat tctggtgacc tccatagctt atttatggtt tggtaaccga
     8521 ttgggaatga atttacgccc ggtactgctg atgcttgagc ggctgaaaga gtgggtgatg
     8581 ttggatatct acctggtcgg cattggcgtt gcttctataa aggtacagga ttatgcccat
     8641 atccaggcgg gggtcggctt gttctctttt gtggcgttgg tgattttaac gacggtgacg
     8701 ttgtcacatc ttaatgtcga agaactgtgg gagcgatttt atccgcagcg ccccgctacg
     8761 cgaagggacg agaaactccg tgtctgtctt gggtgccatt ttaccggcta tcctgatcag
     8821 cgtggtcgct gcccgcgttg ccatatcccg ctactcctgc gtcgccgtca tagtctgcaa
     8881 aaatgctggg cggcgctgtt agcgtcaatc gttttgttgt tacctgccaa cctgttgcct
     8941 atctctatca tttatctgaa tggtggtcgg caggaagata cgattctttc cggaattatg
     9001 tcgctggcaa gtagcaacat tgcagttgcg ggaatcgtgt ttatcgccag tattctggta
     9061 ccgtttacta aagtgatcgt catgttcact ttactgttga gtattcattt taaatgccag
     9121 caaggtttac gcacacgcat tctgttactg cggatggtga cctggattgg tcgctggtcg
     9181 atgctcgacc tgtttgtcat atctttaacc atgtcgctga ttaatcgcga tcagatcctc
     9241 gcttttacta tgggaccggc tgcgttttat ttcggcgcag cggtaatttt gactattctt
     9301 gctgtggaat ggctggacag ccgcttactt tgggatgcac atgagtcagg aaacgcccgc
     9361 ttcgacgact gaagcgcaga ttaaaaataa acgccgtatc tcacctttct ggctgctgcc
     9421 gttcatcgcg ctaatgattg ctggttggct gatttgggac agttatcagg accggggcaa
     9481 tactgtcacc atcgacttta tgtcggcgga tggtattgtt ccaggccgta cgcctgttcg
     9541 ttatcagggc gttgaagttg gaacagtgca ggatatcagc ctcagcgacg atcttcgtaa
     9601 gattgaagtc aaggtcagca tcaagtccga tatgaaagat gcgctgcgcg aagagacaca
     9661 gttctggttg gtgacgccaa aggcctcgtt ggcaggtgtc tccgggctgg acgccctcgt
     9721 cggtggtaat tatatcggca tgatgccggg taaaggtaaa gagcaggatc actttgtcgc
     9781 actcgacacc caaccgaaat atcggctgga caatggcgat ctgatgatcc acctgcaagc
     9841 ccccgatctc ggttcactga gcagtggttc attggtctat ttccgcaaga tcccggtggg
     9901 taaagtctac gactatgcca tcaatcccaa caagcaaggc gtggtgattg atgtcctgat
     9961 cgagcggcgt tttaccgatc tggtgaaaaa aggtagccgt ttctggaacg tttccggcgt
    10021 tgatgccaac gtcagtatca gtggcgcgaa ggtgaaactg gaaagtctgg cggcactggt
    10081 taacggtgcg attgccttcg attcacctga agagtcgaaa cctgccgaag cggaagatac
    10141 ctttggtctg tatgaagacc tggcccatag ccaacgtggc gtaataataa aactggaact
    10201 gccaggcggt gccggattaa ccgccgactc gacgccgtta atgtatcagg ggctggaagt
    10261 cggacagctg actaaactgg atttaaatcc tggtggtaaa gtcactgggg aaatgaccgt
    10321 tgatcccagc gtcgtcaccc tgcttcgtga aaatacccgc atcgaattac gtaatccaaa
    10381 attatccctt agcgatgcta atctcagtgc cctgctgacc ggcaaaacct tcgaactggt
    10441 gcccggcgat ggcgagccac gtaaggagtt cgtggttgtg ccaggcgaaa aagccctgct
    10501 gcaagagccg gatgttctga cactgaccct gacagcacca gaaagttacg gtattgatgc
    10561 tggtcagccg ctcattcttc acggcgtgca ggtaggccag gttatcgatc gtaaactcac
    10621 cagcaaaggc gtcaccttta ccgtcgccat cgagcctcag catcgcgaac tggtaaaagg
    10681 cgatagcaaa tttgtcgtca acagccgtgt cgacgtgaag gtggggctgg atggcgttga
    10741 gtttctcggt gccagcgcct cagaatggat caatggcgga atacgtattc tgccgggcga
    10801 taaaggcgag atgaaagcca gctatccact gtatgccaat ctggaaaaag cgctggagaa
    10861 cagccttagc gatttaccca ccacaaccgt gagtttgagt gcagagacgc tgccggatgt
    10921 gcaggcagga tcggtagtgc tgtaccgtaa atttgaagtt ggtgaagtta ttactgtgcg
    10981 tccgcgagct aacgcgtttg atatcgatct gcatattaaa ccggagtatc gcaaccttct
    11041 gaccagcaac agcgtgttct gggcagaagg tggggcgaaa gttcagctga atggtagtgg
    11101 cctgaccgta caggcatccc cgctctccag agcattaaag ggggctatta gcttcgataa
    11161 cctcagcggt gccagcgcca gtcagcgtaa aggcgacaag cgtattctgt atgcttccga
    11221 aacagcggcc cgtgcggttg gcgggcaaat tacgcttcac gctttcgatg ccggaaaact
    11281 ggcggtcggg atgccaattc gctatctcgg tattgatatc gggcaaatcc agacgctgga
    11341 tctgattacc gcgcgcaatg aagtacaggc aaaggcggtg ctctatccgg aatatgtcca
    11401 gacctttgct cgcggtggta cgcgcttctc agtggtcaca ccgcaaattt cggcagctgg
    11461 cgttgagcat cttgatacta tcctccagcc gtatatcaac gtcgaaccag gccggggcaa
    11521 tcctcgccgc gactttgaat tacaagaggc caccattact gattcgcgtt acctggatgg
    11581 cttaagcatt attgttgaag cgccggaagc cggttcgtta ggtatcggta cacctgtgct
    11641 gttccgtggt ctggaagtag gtacggttac cggaatgacg ctggggacat tgtcagatcg
    11701 cgtgatgatt gcgatgcgca tcagtaaacg ctatcaacac ctggtgcgta acaattccgt
    11761 cttctggttg gcatcgggtt acagtctgga ctttggtctg acgggcggcg tagtgaaaac
    11821 cggcaccttt aaccagttta tccgtggcgg catcgccttc gccacgcctc cgggtacgcc
    11881 actggcaccg aaagcccagg aaggcaagca cttcctgttg caggaaagtg aaccgaaaga
    11941 gtggcgtgaa tggggaactg cgcttcccaa ataatgccca ctgctccggc gtgcctgcgc
    12001 cggagcgttt atgctaaact gcacgcctgt ttttttgcca gtggtatatg ctcgtggccc
    12061 aacacaccgt ttatttcccg gacgcctttc tgacacaaat gcgcgaagcg atgccttcga
    12121 cgctctcttt tgatgatttt cttgccgcct gtcagcgccc gttgcgccgc agcattcgcg
    12181 ttaatacgct gaaaacctcc gttgctgatt tcctgcaatt aaccgctcct tatggctgga
    12241 cgcttacgcc aattccgtgg tgtgaagaag gtttctggat tgaacgcgac aatgaagatg
    12301 cattgccgtt gggtagtacc gccgagcatt taagcggcct gttttatatt caggaagcca
    12361 gttcaatgtt gcctgttgcc gccttgtttg ctgacggtaa tgcaccacag cgggtgatgg
    12421 atgtcgctgc tgcaccaggt tccaaaacga cgcaaattgc cgcgcggatg aataacgaag
    12481 gggcaatcct tgccaatgag ttttccgcca gtcgggtaaa agtgttacat gccaatatca
    12541 gccgctgtgg catcagtaat gttgcgctca cgcattttga tggccgcgta tttggtgcgg
    12601 cagtgccaga aatgttcgat gccattttgc tggacgctcc ctgctccggc gaaggcgtgg
    12661 tgcgtaaaga tcccgatgcg ctaaaaaact ggtcaccaga aagcaatcag gaaatcgcag
    12721 cgacccaacg ggagctgatc gacagcgcct ttcatgcatt acgtcctggc ggtacgctgg
    12781 tttactcgac ctgtacctta aaccgggaag aaaacgaagc cgtttgcctg tggctgaaag
    12841 agacttaccc cgacgcagta gagtttttac cgcttggcga tctcttccct ggtgcaaaca
    12901 aggcgctgac cgaggaaggc tttttgcatg ttttcccaca aatttacgac tgcgaaggct
    12961 tcttcgttgc tcgtctgcgt aaaactcagg cgatccccgt cttacccgcc ccaaaataca
    13021 aagtgggcaa tttcccgttt agcccagtga aagatcgcga agccggtcaa attcgtcagg
    13081 cggctgcaag tgttggctta aactgggatg aaaacctgcg actctggcag cgtgacaaag
    13141 aactgtggtt gttcccggtg ggcattgaag ccctgatcgg taaagtccga ttttctcgct
    13201 tggggattaa acttgccgaa acgcacaaca aaggttatcg ctggcagcat gaggccgtta
    13261 ttgcccttgc ctcccccgac aatgtgaacg cttttgaact gacaccgcag gaagcggagg
    13321 agtggtatcg cgggcgcgat gtatacccgc atgccgcgcc agtggcggat gatgtattgg
    13381 ttactttcca gcatcagccg attggtttag ccaaacggat tggttcacga ctgaaaaaca
    13441 gctacccgcg tgaactggtg cgcgacggga aactttttac cggtaacgcc tgacagcgca
    13501 caaaaaagcg cactttttga ctggcacatt cgactgcctc aactaggctg aaaaatggtg
    13561 cgatcggact ggtcgtacca caaccggcag ctaaatggag agcacgaaga tgaaaaccag
    13621 tgtgcgcata ggcgcttttg aaatcgacga cggcgaatta cacggcgaat cgccgggtga
    13681 tcgaacgtta accattcctt gtaaatcaga ccccgattta tgtatgcaac tggatgcctg
    13741 ggatgctgaa accagtatcc ctgccctgct aaatggcgaa cactctgtcc tttaccgtac
    13801 ccgttacgat caacaatctg atgcctggat tatgcgtctt gcctgatcca aaaagaaccc
    13861 gtcggcatgg cgggttattt gtccgggtta ttcccccgtt gtaaaatctc tcctaaactt
    13921 aacggtacgg caccacactt cggggatgaa atgttcgcgc tggtactttt tgtttgctac
    13981 ctggatggcg gttgtgaaga tattgttgtg gatgtctaca acacggaaca gcagtgtctt
    14041 tattctatga gcgatcaacg gatccgccat ggcggttgtt ttccgattga ggattttata
    14101 gatggtttct ggcgacctgc acaggagtac ggtgattttt aattattgca attgcacaag
    14161 agtcagttct cccccaaaga cagcaccggt atcaatataa tgcaggttgc caatatccac
    14221 gcgatggcgc aaaggtgtat gaccaaacca gaaatgatca gcacctgcaa ttccctgccc
    14281 tttttggcgt tcacctaatc gcgagcggct ccacaagacc tgatgcaaat cgacgtcctt
    14341 ttgccattca taaacatcat ctggataatc ggcatgagca ataacatgtt tgccggtgcg
    14401 gctgtgtact tcaagaataa agggcaaatg ctgacatttt tccagcgccg ttttcgcttg
    14461 tttctgttga ttatctgcca gcgcaataaa ccagtcgccg ccattcatca accataaaga
    14521 catctgccgg gatgccagcg catccatcgc catctgttca tgattgcctc ttaccgcacg
    14581 aacccaatgt tgttccagta actgcaggca acgtaaactt tgcggcccac gatcgataac
    14641 gtctcccact gagataagta aatctcgcca cggatcaaaa cgacaatgcc ataatttgcg
    14701 gcgcaattgc tcaagacaac cgtgtatatc gcctgaaagc cagatatgtc gccattgatg
    14761 acccgcaatt ctctgataaa cgggcgcagg ctgtttcatc aatattttcc tcccgcgcta
    14821 aagatcacat aatcttaaca agaatgttaa aaaacgctgg actcaaacag tagagtgtgt
    14881 gttatggttg actataaagt cagcgaaggg aatgcttctg gcttttaaca gataaaaaga
    14941 gaccgaacac gattcctgta ttcggtccag ggaaatggct cttgggagag agccgtgcgc
    15001 taaaagttgg cattaatgca ggcttagttg ccttgctctt taagaataga tgacgacgcc
    15061 aggttttcca gtttgcgtgc aaaatggtca ataaaaagcg tggtggtcat cagcttaaat
    15121 attaaaaacc gcccgttctg gtgaaagaac tgaggcggtt ttttattgga aatcaaaagg
    15181 ctattttagg taattaacag agtttttcag ctcgctctat aaacggtgcc agactcattt
    15241 tttcgccggg attgttagga tcatcaatct gaatcaccga aatgggttgg gctttagtct
    15301 tcccactggc aacttccttt tgtgcgatat cgtttaaagg atactgcacg agagtacttg
    15361 ggttaatgac atacaaagca ttacccggtc ggcaagtcag catcacctct tcgcgattaa
    15421 acgcccattt gtctttacct acttcaaaac ggctgacggt aatcacctgc ggtgccgcca
    15481 gcgcagctgc agaactggtg agtaacagaa acgccagaat actttttttc atcattttcg
    15541 taatccatca aaaaggttcg agagtcgcaa gcaggctgat gatcaccagc actaccgcac
    15601 ctatcgccca ttcgagttta gtcatccaga caaaccaggg agccgcacga tcttcatcct
    15661 gcctcatgcg cggtacaaga acataacgat tagccagcgc gatgaccacc ataatcatca
    15721 ccaggatggc tttgagcaat aagagttggc cccagtgagt cgtaagcgtg gggggaaatc
    15781 cggtaatcaa caatgcatta agcacgccgc ttgccagtac gccgatcacc gcaaaatgcc
    15841 cgcaccagga aaaacgcatc agcgcctgaa tagcctggtg tcgccaacgg cctttgatga
    15901 gttgcataca ccagagcacc ggcagtaatc ccccaaacca cgccgccgca cagatcaggt
    15961 gaattgcatg attagtctga tggattttcg ccgttacccc ttcattcagc gtcgcatgac
    16021 cgactcccgc cagcagaata aattgcgcgg tagtgagcat aaatagcaaa cgtggcatat
    16081 tctgcggttg cataagggcg acgatcaatg ttacgagggc gagaacaatt tgccataacc
    16141 agacgccacc aaactgcgtt tgcaaaacag ccagccaaat gtttggcgaa aatacatctg
    16201 accatcccgt tcccatcaac ccgccctgaa cggcgagcat tgcagtggca ctgatcaaac
    16261 tccagacggc agcgtgctgt tgtaaacgca gaaaacgctt cgtcagcaag cgacgaatcg
    16321 ttaagggggc cagccaggca ccgtacattg cgaagccaaa aaccagcatc agcgaggtga
    16381 aatggataaa tcgcagcgcg atccaggtaa acgccagcat gatttatttc acgctaaagg
    16441 tgtaatgtcc tttcgtttta tgcccatcca ccgaaacaac atgccagtca acggtgtagg
    16501 ttccgggttt cagcgaatcg gccagtggaa caatcagttg cttttggtcc tgctcattac
    16561 gcttcgccgg taatgtttta atattttcgt tttttggccc cgtgattttt gcgccactga
    16621 atcctgtttc aacgccttcc gagaagttta aagtgattgc ctgcggagct gctgtcactt
    16681 gtgcatttgc cgcaggatac tgatgcgtta aatgcgcatg tgcccagacg gaaggtgtca
    16741 ccaacgaagt ggtcaggatt gccagcgcgt agcgaaggga gcgtgcagtt gaagccatat
    16801 tatctattcc tttttgtaat aactttttta cagagcataa ccttgtctaa tgtctgagtc
    16861 gaggatcatc aattccggct tgccatcaag gctcactctt agtaactttt gcccgcgaat
    16921 gatgaggaga ttaagaatgc tgaagaatct ggctaaactg gatcaaacag aaatggataa
    16981 agtgaatgtc gatttggcgg cggcaggggt ggcatttaaa gaacgctaca atatgccagt
    17041 gatcgctgaa gcggttgaac gtgaacagcc tgaacatttg cgcagctggt ttcgcgagcg
    17101 gcttatcgcc caccgtttgg cttcggtcaa tctgtcacgt ttaccttacg agcccaaact
    17161 taaataaaac ttatacatag ttacactttc ttacataatg cctgctaaat tatgagtatt
    17221 ttctaaaccg cccccataat ttgcagtcat tttgaaaagg aagtcattat gtcgttctgg
    17281 aaagttgcag cagcgcaata tgagcctcgt aaaacctcac tcaccgaaca ggtagctcat
    17341 catctggagt ttgtgagggc cgccgccagg caacaatgtc agctgttagt ttttccatcg
    17401 ctttctttac tggggtgtga ttattcgcga cgtgcccttc ctgccccacc cgatctctcg
    17461 ctattagacc cgctttgtta tgccgcaacg acctggcgaa tgaccatcat tgccggcctt
    17521 cccgttgaat ataacgatcg ctttattcgt ggaattgcgg tattcgcacc gtggcgaaaa
    17581 acgccaggga tctaccatca gagttatggt gcatgtctgg gccgtcgttc cagaaccatc
    17641 acggtggtcg atgaacaacc gcaaggtatg gatatggacc cgacctgttc actgtttacg
    17701 acagggcaat gtctgggaga acccgacctg ctggcgtcgg cccgtcgctt acaatttttt
    17761 tcacatcagt actcgattgc tgtgctgatg gcaaatgccc gtggtaacag tgcactatgg
    17821 gatgaacatg gtcgtctcat cgttcgcgcc gatcgtggtt cattattgtt agtcggtcag
    17881 cgttcatcac agggttggca aggcgatatc attccattac gctaggcttt ttcggcctgg
    17941 agcatgccat gttgcgcatt atcgatacag aaacctgcgg tttgcaggga gggatcgttg
    18001 agattgcctc tgttgatgtc attgacggaa aaatcgtcaa tcccatgagc cacctggtgc
    18061 gtcccgatcg tcctattagt ccacaggcga tggcgattca tcgcatcacc gaagccatgg
    18121 tcgccgataa accgtggatt gaagatgtga tcccgcacta ttacggtagt gaatggtatg
    18181 tcgcgcataa cgccagcttt gaccgccgcg tactgcctga aatgcccggt gagtggattt
    18241 gcactatgaa actggcccgt cgtttgtggc cagggatcaa gtacagcaat atggcgttat
    18301 ataaaacacg gaagctcaat gtacagacgc cgccgggcct gcatcatcac cgcgcgttgt
    18361 atgactgtta tatcaccgct gcgttgctta tcgatattat gaacacctcc ggctggacgg
    18421 cagaacagat ggccgatatc accggacgtc catcgttgat gacgactttc acctttggca
    18481 aataccgtgg caaagcggtt tccgacgttg ccgaacgcga tccgggctat ctgcgctggt
    18541 tatttaataa cctggacagc atgagcccgg agctgcgttt aacactgaaa cattatctgg
    18601 aaaatactta atccgcaggc gtagcgggta atgttccctg cgccagcgcg accagaaaag
    18661 catattccat cgctacgcct tcgtacgatt taaagcgacc agatttaccg ccatggcctg
    18721 agtccatgtc ggtacagagc aataaaagat ggttatcggt tttcagctcg cgcaatttag
    18781 cgacccattt tgccggttcc caatattgca cctgagaatc gtgtaaaccg gtcgttacca
    18841 gtaaatgcgg ataagcctgt gcggtgacgt tgtcatacgg gctgtagctt ttcatatact
    18901 cgtaatattg cggatcctgc ggattccccc actcttcaaa ttcgccggtg gtaagaggaa
    18961 ttgattcatc cagcattgtt gttacaacat caacaaacgg cacctgggcg ataacgccgt
    19021 ggaacaattc cgggcgttga ttaatcgcaa cgcccatcaa catacccccc gcactcccgc
    19081 ccatcgcata acagagcgaa ggagagccat agcccagttt taacaatgca tcgcaggcat
    19141 caagataatc attaaacgta tttttcttct tcagaaattt accgtcctcg taccattgtt
    19201 gccccagctc accgccgcca cgaacatgga caatggcgta gacaaatcca cgatccaaca
    19261 aactcaagcg gctaaaactg aaatcggcat caatacttgc gccgtaagaa ccgtagccat
    19321 acaccagcag cgggttgtgt cctttgcgaa aatgtttgcg atgatagacc agcgaaaccg
    19381 gaacttcgac gccatcacgg gcgactatcc acaggtgttc actgcggtaa ttcgccgcat
    19441 caaaaccagg aacttccgtt tgttttaata cacgacgctc accggtatcc atatccagtt
    19501 caaacaaagt atctggtgtg gtcatggacg aataaccata acgcaatcgc gcggtttcag
    19561 gttctggatt gtaggcaatc caggtcacat aggccggatc atcaaaggca atgccaatga
    19621 cttcccgggt cttgcggtta atttgccgca aactggttaa cccgcgctga cgctcttcaa
    19681 ccaccagcca gtcggtaaac agcgtaaacc cttccagcat aatgttatca cgtggcggaa
    19741 ttaactcttc ccactgttgc tcatcacgca tacgggtacg gtataagcca aagtttttac
    19801 cgtggcggtt ggaacgcaga taaaaccgat gctggtagtg atcaaggctg tattcgtgat
    19861 ctttgcggcg cggcagaaaa acaaacggct cggcatcggc tatttccgcg tccagcaggc
    19921 gaacttcact ggtggtggcg ctggccagat gaatgactac atagtgcttc gaggtcgttt
    19981 tatgcaggct gacgtaatag gtatcgtctt tttcttcgta gatcagttta tcctgcgatg
    20041 ctggcgtacc gatggcgtga cgccagacct gataaggaag cagcgtcacc ggatgcttgc
    20101 gaacatagta gaaagtccag gaatcatttg cccagacaaa gctggggtca acgttatcca
    20161 gcagttccgg ataccagtta cctgtttcca gattacgaaa acgaatgccg tactggcgtc
    20221 gggaaagaaa atcttctgcc agtgccataa tggtgttatc gggcgtaatc gccattccgc
    20281 ccatcgaata aaactcacta tgagccgcgc gcttgttggc atcgagcaat gtttcccact
    20341 catcccactc ttcgctgaac gccgattgac gctggtagat agcatattca cagcctggtt
    20401 cataaatatg ccgatagcgg tagccatttt ttatgtaggg cgcagaaact tctctttgcg
    20461 gaatgcggtc gatgatttcc tttaagatgc gatcctgcaa ggcttgttgc gaggccatca
    20521 cccgatgacc gtaactattt tcttgttgca gatagtccag gacttccggc tgagaacgcg
    20581 tatcgtcccg cagccagtag taattatcga tgcgcgtatc gccatgaagc gtcatggcgt
    20641 ggggaatgcg ggcggctttt ggtagcatgt tattgttctt tctggttgaa acatcttata
    20701 agggtggcaa aactcaccgg ggatgcaagc gaaacagggt agtcattgct tagatgatga
    20761 caggtaatgg cgcggatatc gactgttatg cagacagaga aaatcagcct gttcaaatgg
    20821 ctgtgcggtt ctggataacc cgaaatagtc aacttcaggc tatccagaga gcggaattac
    20881 tccgccaaag tacgtttttg ctgttcgaga tcgcgttcaa tgccttcacg aacgtcctgg
    20941 gggattttca gcgcatcacc cagtgcattc aggtaactgc gttccataaa atggtcaata
    21001 tcaatagccg cgcagctcag gaaatagatt tccagcgcct cttcttcatt gcggaccccg
    21061 gtagccaggc gttgtggatc cagcggttgt tcgattgcct gctcaatgag tacacgcccc
    21121 ttctcttcca caccggcttc acgcaattgc aggtcgattg ccgcacgttc tttggcatca
    21181 atatgaccat cacttttagc ggcaaagacc agcgcaagga tcaaacgttc cgtacgctca
    21241 tccagcggcg tactttgcgc gccaaactgc ggttcgtcct gatgcgccgc gcgaatttta
    21301 tctttgtatt tattccacag caccgtaccc gctaccgctc cgccgccaac cagtaacgcg
    21361 tttgtgccat atttcgtaag aagtttacgt gctgatttat ttgcgaccag cagcccagcc
    21421 agtccgccta atgcgccagg cactaaaagt ttgcccaatc cctgatccgc agacgaggag
    21481 gtagaagaac ggctttgccc aagcagggat tgcagttgat ttaaccagtt agccatattt
    21541 gctcctcaat aaccattttt agtgcctgta gcatagcgga tgaggatgtc agaaaatgta
    21601 tatcgagaca aaagatgcgc aaataagcgg ggcggtaggc cgttattcaa agaaattcgc
    21661 gccaggtaag gtgttcactg tatataaggt gataagggta ttttaggtga aagtagtttg
    21721 acattttgag tggaactgtt tgatgctgat tgcccggttc gacgctactg cgtcttctca
    21781 ggcctacgat tgtgttgcgt tgtagggcgg acaaggcacc cgcgccgcat ccgccaacgg
    21841 tcaatcgaat tatcagaagc cattaacgcc gctgatattc cgccattccc gctttgcagt
    21901 ccacgctgac ctggtaatga atatcggcac ttttaccacg cacggttagc ggtactgacc
    21961 atttatcatc tttcccctga atgtcctgca aactgaccca tgccacggga tcggcctgac
    22021 cgacaatttt ttgatcatca gcccagcgcg ccacgcgatt ttgttggtaa tcacgtttta
    22081 cgctcgcggc aattccggca gcatccagac cttcacactt tgggaaagtg accgacttgc
    22141 tggtttcatt attggcagcg aaaactgatg cgcaggcaga aaccaacaac agccctaaaa
    22201 acgcccctct ttttttcatg tttttctcca tagcacaatg attcaggaga aagcatggta
    22261 caaattgtca ggagcgcaag ttgcttcagg ccgcgtgtga ggcatcttcc tcttcggatt
    22321 cagcaccgac gacctgtggg gacggtaatt tgccggtttt cagaatggtg ctcagcacat
    22381 ctttttgttc tgccagccat agcgaaaggg cttcacgttg ctcgtcttcc atttgcactg
    22441 gagaatttgt cagccaggtg tcaagcagat cggccgtatc aagcatcttg tcataagcat
    22501 cggcttcctt tttgctggta aacgacattt tctcttcgcc ctcacgaatg actacgtatt
    22561 taacttcaac cgccatttgc agcctctcat aataactgtg gttttataca gtatatttct
    22621 tttcagttgg gaaatcaaca tcagcaataa agacacacgc aaacgttttc gtttatactg
    22681 cgcgcggaat taatcagggg atattcgtta tgacgttatt aggcactgcg ctgcgtccgg
    22741 cagcaactcg cgtgatgtta ttaggctccg gtgaactggg taaagaagtg gcaatcgagt
    22801 gtcagcgtct cggcgtagag gtgattgccg tcgatcgcta tgccgacgca ccagccatgc
    22861 atgtcgcgca tcgctcccat gtcattaata tgcttgatgg tgatgcatta cgccgtgtgg
    22921 ttgaactgga aaaaccacat tatatcgtgc cggagatcga agctattgcc accgatatgc
    22981 tgatccaact tgaagaggaa ggactgaatg ttgtcccctg cgctcgcgca acgaaattaa
    23041 cgatgaatcg cgagggtatc cgtcgcctgg cggcagaaga gctgcagctg cccacttcca
    23101 cttatcgttt tgccgatagc gaaaaccttt tccgcgaagc ggttgccgcc attggctacc
    23161 cctgtattgt taaaccggtg atgagttctt ccggtaaagg gcagacgttt attcgttctg
    23221 cagagcaact tgctcatgca tgggagtacg ctcagcaagg tggtcgcgcc ggagcgggcc
    23281 gcgtaattgt tgaaggcgtc gttaagtttg acttcgaaat taccctgcta accgtcagtg
    23341 cggtggatgg cgtccatttc tgtgcaccgg taggtcatcg ccaggaagat ggcgactatc
    23401 gtgaatcctg gcaaccacag caaatgagcc cgcttgccct tgaacgtgcg caggagattg
    23461 cccgtaaagt ggtgctggca cttggcggtt atgggctgtt tggtgtcgag ctgtttgtct
    23521 gtggtgatga ggtgattttc agtgaagtct cccctcgccc gcatgacacc gggatggtga
    23581 cgctaatttc tcaggatctc tcggagtttg ccctgcatgt acgtgccttc ctcggacttc
    23641 cggttggcgg gatccgccag tatggtcctg ctgcttctgc cgttatcctg ccgcaactga
    23701 ccagtcagaa tgtcacgttt gataatgtgc agaatgccgt aggcgcagat ttgcagattc
    23761 gtttatttgg taagccggaa attgatggca gccgtcgtct gggggtagca ctggctactg
    23821 cagagagtgt tgttgacgcc attgaacgcg cgaagcacgc cgccggacag gtaaaagtac
    23881 agggttaaac ccggcaaaaa aacgctacaa aaatgcccga tcctcgatcg ggcattttga
    23941 cttttacagc ttagcgcctt ctacagcttc acgcgccagc ttagtaatgc ggtcgtaatc
    24001 gcccgcttcc agcgcatctg ccggaaccag ccaggaacca ccgatgcaca gcacgctttt
    24061 cagcgccagg tagtcacggt agttagccgg agaaatacca cctgtcgggc agaaacggac
    24121 ctgggagaac ggacccgcga tcgcctgcag ggctttcaca ccgccgttag cttcagccgg
    24181 gaagaatttg aactctttca aaccgtagtc catacccagc atcagttcgg aaacagtgct
    24241 gatccccgga atcagaggaa tagtcccttc ggtagcagct ttcagcagcg gctcggtcag
    24301 acccgggcta attgcaaact gtgcacctgc ttcagtgact tctgccagct gctgtggatt
    24361 cagcaccgta ccggcaccca caatcgcttc aggcacttct ttggcgatag cacggatagc
    24421 gtcaactgcg cactcggtac gcagagtcac ttccagaacg cgcaccccac cagcaaccaa
    24481 cgcttttgct atcggcaccg cgtgttccag tttttttacc acgataaccg gtacaaccgg
    24541 gccggtggtc aggattgatt ctgcacttgt tttccagttt ttcatcagag ttttctctcg
    24601 cctgattaca aatttgtcgt cttaaaaagt gatacaggtt gcgccctgtt cggcaccgga
    24661 cagtttttca cgcaaggcgc tgaataattc acgtcctgtt cccacgcgtg acgcgctcag
    24721 gtcaggaatg tgcggttcgc gagcagccag ttccgcttcg tctaccagca gcgtcagttc
    24781 gcctgtctgt ccattcacac gaatgatgtc cccgtcgcgc acttttgcca gcagcccgcc
    24841 atcgtaggcc tctggtgtta cgtggatagc tgacggcact ttacctgaag cgccggagag
    24901 tcgtccatcg gtaactaacg caattttgaa acaccggtcc aataatacac caagtggcgg
    24961 catgagttta tgtaattctg gcattccgtt cgcttttggc ccctgatgac ggacaacaac
    25021 gacacaatcg cggtccagta aacccgcttc aaaggccggc ataacgtcat gctggctttc
    25081 aaaaacaacc gctggcgctt caatcacctg gttctctacc ggcacggcag aggttttcat
    25141 aaccgctcgc cccaggttac cgcttaacac ttttgtccca ccatgatgag agaaaggttg
    25201 ttcgaaggac gcgatcacat tgttgtcgag tgatttttct gccccttccc gccagtccag
    25261 ttcaccatta ttcagccatg gttcaagggt ataacgagac agaccaaagc ctgccaccgt
    25321 attgacatct tcatgcagca ggcctgcttt gagcagttca cgtaccagaa ccggtacgcc
    25381 acctgccgcc tggaagtggt taatatcggc cggaccgttc gggtagagac gtgccatcag
    25441 cggtacaaca tcagaaaggt cagagaagtc atcccagtta atctgaatac cggctgcgcg
    25501 cgccatcgcc actaggtgca tggtgtggtt agtggaacca ccggtcgcca gcagtgcaac
    25561 gataccgttc accaccactt tctcatcgat catcttaccg atcggcatcc attcattacc
    25621 attaccggtc atgcgcgtaa cctggcgcgc agctgcagcg gtcaaagcat cgcgcagcgg
    25681 agaatccgga tgaacaaaag aagagcctgg caactgcatc cccataaact ccaccaccat
    25741 ctggttggtg ttggcagtac cgtagaaagt acatgttccc ggcgcatgat aagacgcggc
    25801 ttctgactcc agtaatgcca tgcggtccac tttaccttcg gcataaagct ggcgaatacg
    25861 cactttttct ttatttggca aaccgcttgc catcggtcca gacggcacaa acaccgcagg
    25921 caaatgacca aacgacaggg ctgccatggt cagacctggg acaatcttgt cgcacacacc
    25981 gagaaacaga gcaccatcaa acatgttatg ggacagcccc accgccgcag acatcgcaat
    26041 cacttcgcgg cttaacagcg acaattccat tccatcctgc ccctgggtga caccatcaca
    26101 catcgccgga acaccgcccg caacctgacc aaccgcattc gcttcatgca gggctttacg
    26161 aatgatttct ggatagtgtt cataaggctg gtgagcggag agcatgtcgt tataggaggt
    26221 gatgatggcg atattgttac gcaacatgct tttcaaagag gctttgtctt ctggctggca
    26281 ggcagcgaaa ccgtgtgcca ggttaccgca tgccaactgc gaacgatgaa cggtcgaagt
    26341 tttcgcttgt tctatccggg cgaggtaagc agagcgagtc tcgcgcgaac gttcaatgat
    26401 tcgatttgtt acgcgtaaca attgtgggtt cataaaggct cctgaaattg agttgtcaga
    26461 gcaggatgat tcacaacgcg tttcatacat ggaaattatg attgaaacgc ctgtaaccgg
    26521 agctcatagg gcaaaaacgt ttcagtcagt gtaataaaaa aagcctcgtg ggtgaatccg
    26581 cacgaggcct gaaagtgtaa aaattgttct acaatctgtg caagatcatg ttaccggtaa
    26641 aataaccata aaggatgagc gcagatatta ctcaaactca ttccaggaac gaccatcacg
    26701 ggtaatcatc gccactgagg caacgggtcc ccaggttccg gcctgatacg gtttcggcgc
    26761 atcattgtcc atcgcccacg cctcagtaat ggagtctacc catttccagg cttcttccac
    26821 ttcgtcgcga cgtacaaaca gtgcctgaat accacgcatg gtttccagca gcaaacgttc
    26881 ataggcatcc gccagatgcg tctgattaaa ggtttctgaa tagctcagat ccagcttggt
    26941 gatttgcagg ttatgtttgt ggtcaaggcc aggaacttta ttcagtacct ggatatccac
    27001 gccttcatca ggttgcagac ggatagtcag tttattttgc ggtagatcct gccacgactc
    27061 tttaaacaga ttcagttcag gtgttttgaa atagaccacg acttcagaac atttggtcgg
    27121 cagacgttta ccagtacgca ggtagaatgg cacaccggcc cagcgccagt tatcaatgtc
    27181 gacgcggatc gccacgaagg tttctgtatt gctgctcttg ttcgcgccct cttcttccag
    27241 atagcccggc acttttttgc cctgagcgaa gcccgcagta tattgcccgc gtacggtttt
    27301 ttcgcgcaca ttggagcggt cgatgcggcg cagagacttc agtactttca ctttttcatc
    27361 gcggatgctg tctgcgctca ggtcagacgg cggagacatc gcaatcatgc aaagaatttg
    27421 cagcaggtgg ttctggatca tgtcgcgcat ctgaccggct ttatcaaaat agccccagcg
    27481 cccttcgatc cccacttctt ctgccacggt aatctcaaca tgatcaatgg tgcgattgtc
    27541 ccagttgttc acaaacagag agttagcaaa acgcagcgcc aacaggttca gcactgtttc
    27601 tttaccaaga tagtggtcga tacggtaaac ctggcactcc tcgaagtatt cgccaacctg
    27661 atcgttgatt tcctgcgagg tcgccagcga cgtccccagc ggtttttcca tgactacgcg
    27721 tgccggttta gcattcagtt ttgcctcacc aagccctttg caaattgcgc caaaagtgct
    27781 gggcggcatg gcaaagtagt taatggtgat acgatttttt tgatccagca tcgcgccgag
    27841 acggctgaat gcagcagtgt cattgacatc aagattacaa aaatccagac gtgcactcag
    27901 ggtgtcccat aaaccttcat caatggtttc tttcatgaaa gtttcgagcg cctcgcggac
    27961 aactttggta taagccgctt tatcccagtc agcacgccct acgccgataa tccgggtgtc
    28021 cgggttgagc tgaccggctt tttccagttg atacagggaa ggcagcaatt tacgacgcgc
    28081 aaggtcgcct ttcgcgccga aaatgaccag gtcacaggcc tgggctgttt gcgttaccgc
    28141 catgtcattc tccttaagtt aactaacccg gtacttaagc cagggtatac ttgtaatttt
    28201 cttacggtgc actgtactgc ttttacgagc ttgcgaaaac tgtaaacgct tatccacccg
    28261 tgcgattacg gtaaaagcgc gcaaagtgcg gcaaaactga taaaaaaatc atcgttttcc
    28321 attgggtaaa aatctgacac tgatcatgtt atgaaaaaaa ataacaactt ttttatctgc
    28381 ttttgtcatt aacacagcac acaggcgtaa tatctgacaa aactgcattt cgatttcttt
    28441 cagagcggaa atcgtcatta cccgtgagtc tctttacatc atgaatatgc tggaaaaaat
    28501 ccagtctcag ctggaacatt tgagcaaatc tgagcgcaaa gttgccgagg tcattctggc
    28561 ttcgcccgat aacgcgatcc attcgagtat tgctgctctg gcgctggaag ccaatgttag
    28621 cgaaccgacg gtgaatcgtt tctgtcgcag catggacaca cgcggttttc ctgattttaa
    28681 acttcatctg gcacaaagtc tggcgaatgg cactccctat gttaatcgca acgtcaatga
    28741 agatgacagc gttgaatcat acacggggaa aatatttgag tccgcaatgg caacgctcga
    28801 tcatgtccgt cattcactgg ataaatctgc catcaaccgc gccgtcgact tgctcactca
    28861 ggcaaaaaaa atcgcctttt tcggattagg ttcttcagcc gccgttgccc acgatgcgat
    28921 gaataagttc tttcgtttta atgttccggt ggtgtactcc gatgatatcg tgctgcaacg
    28981 catgagttgt atgaattgta gcgacggaga cgtggtggtg ctgatttctc acacgggaag
    29041 aacaaaaaat ctggtcgagc tggcgcagct ggcacgcgaa aacgacgcta tggtaattgc
    29101 cctcacctct gcgggtaccc cactcgcccg ggaagcaacg ctggcaatta ccctcgacgt
    29161 accggaagat actgacattt atatgcccat ggtttctcga cttgcacagc tgaccgtgat
    29221 agatgtgctg gcgacaggat ttactttgcg gcgcggtaca aaattcagag ataacttgaa
    29281 gcgggtcaag gaagcgctga aggaatcgcg ttttgataag cagttactta atttaagtga
    29341 cgatcgctaa aaacgactgt cactgtccta atcttatact acatccgaat gagattaatt
    29401 tatcgccatc acggcgttat ttcattcgga tttcatgttc aagcaacacc tggttgtttc
    29461 agtcaacgga gtattacatg tccagaaggc ttcgcagaac taaaatcgtt accacgttag
    29521 gcccggcaac agatcgcgat aataaccttg aaaaagttat cgcggcgggt gccaacgttg
    29581 tacgtatgaa tttttctcac ggctcgcctg aagatcacaa aatgcgcgcg gataaagttc
    29641 gtgagattgc cgcaaaactg gggcgtcatg tggctattct gggtgacctc caggggccca
    29701 aaatccgtgt atccaccttt aaagaaggca aagttttcct caatattggg gataaattcc
    29761 tgctcgacgc caacctgggt aaaggtgaag gcgacaaaga aaaagtcggt atcgactaca
    29821 aaggcctgcc tgctgacgtt gtgcctggtg acatcctgct gctggacgat ggtcgcgtcc
    29881 agttaaaagt actggaagtc cagggcatga aagtgttcac cgaagtgacc gtcggtggtc
    29941 ccctctccaa caataaaggt atcaacaaac ttggcggcgg tttgtcggct gaagcgctga
    30001 ccgaaaaaga caaagcagac attaagactg cggcgttgat tggcgtagat tacctggctg
    30061 tctccttccc acgctgcggc gaagatctga actatgcccg tcgtctggca cgcgatgcag
    30121 gatgtgatgc gaaaattgtt gccaaggttg aacgtgcgga agccgtttgc agccaggagg
    30181 caatggatga catcatcctc gcctctgacg tggtaatggt tgctcgcggc gacctcggtg
    30241 tggaaattgg cgatccggaa ctggtcggca ttcagaaagc gttgatccgt cgtgcacgtc
    30301 agctaaaccg cgcggtaatc acggcgaccc agatgatgga atcaatgatt actaacccga
    30361 tgccgacgcg tgcagaagtc atggacgtag caaacgccgt tctggatggt actgacgctg
    30421 tgatgctgtc ggcagaaact gctgctggtc aatacccgtc agaaactgtt gcagccatgg
    30481 cgcgcgtttg cctgggggcg gaaaaaatcc cgagcatcaa cgtttctaaa caccgtctgg
    30541 acgttcagtt cgacaatgtg gaagaagcca ttgccatgtc agcaatgtac gcggctaacc
    30601 acctgaaagg cgttacggcg atcatcacca tgaccgaatc gggtcgtacc gcgctgatga
    30661 cctcccgtat cagctctggt ctgccaattt tcgccatgtc acgacatgaa cgtacgctga
    30721 acctgactgc tctctatcgc ggcgttacgc cggtgcactt tgatagcgct aatgacggcg
    30781 tagcagctgc cagcgaagcg gttaatctgc tgcgtgataa aggttacttg atgtctggtg
    30841 acctggtgat tgtcacccag ggcgacgtga tgagtaccgt gggttctact aataccacgc
    30901 gtattttaac ggtagagtaa gtacgttgcc ggatgcggcg aaaatgcctc atccggccta
    30961 cagttcaatg ataattcaac agatttcgaa tcttctgaag caaacttgaa cttatcatca
    31021 gtcgaaggcc tctcctcgcg agaggcattt tttatttgat gggataaaga tctttgcgct
    31081 tatacggttg gatttcgccc ggtttgcgag ttttcagcaa ttttaatatc caggtgtatt
    31141 gttctggtcg cggaccaaca aaaatctcga cttcttcatt catccgccgc gcaatcgtat
    31201 gatcatccgc ctctaacaga tcatccatcg gtgggcgcac ctgaatcgtc agacgatgcg
    31261 tcttgccatc ataaatcgga aacagcggta caacgcgcgc acggcacact ttcatcaaac
    31321 gaccaatcgc gggcaacgtc gctttatagg tggcaaagaa atcaacaaat tcgctgtgtt
    31381 ctgggccatg atcctgatcg ggtaaataat atccccagta accctgacgt accgactgga
    31441 tgaatggttt aataccatca tttctcgcat gcagacgacc accaaagcga cggcgcaccg
    31501 tgttccagac ataatcaaaa accgggttgc cctgattatg gaacatcgct gccattttct
    31561 gcccttgcga ggccatcagc atggcaggaa tatcgacggc ccaaccgtgc ggcaccagaa
    31621 aaatcacttt ctcgttatta cgccgcatct cttcgatgat ctccagccct tgccagtcaa
    31681 cgcgcggctg aattttctcc ggcccgcgta ttgccaactc agccatcatt accatcgctt
    31741 gcggcgcggt ggcaaacatc tcatctacaa tcgcttcgcg ttcagcttca ctacgttctg
    31801 gaaagcagag cgacagattg attaacgcac gacggcgtga gctttttccc agtcgtccgg
    31861 caaaacgtcc cagccgtgcc agaatgggat cacggaactt tggcggcgtt aaagcgatac
    31921 ccgccatcgc tgctacgccc agccatgctc cccagtagcg cgggtggcga aaggatttat
    31981 caaactcagg aatgtattcg ctattatttt ttttcgtttc catgcttttc cagtttcgga
    32041 taaggcaaaa atcaatctgg tgatagtgta gcggcgcaac ttgccccgca ccaaataaaa
    32101 aagccggtac tgactgcgta ccggctgcga atggatgtta attaatcaaa ccgtagctgc
    32161 ggcacaatct ctttggcctg tgccaggaat tcgcgacgat cggagccggt cagcccttcg
    32221 gtacgcggca gttttgccgt cagcgggttt acggcctgct ggtttatcca tacttcatag
    32281 tgcagatgcg gcccggttga acgtccggta ttaccggaaa gcgcgatacg gtcgccacgt
    32341 ttcaccttct gtcccggttt caccaggatc ttgcgcaagt gcatataacg cgtggtgtag
    32401 ctgcgaccat gacgaatagc cacataataa cctgctgcgc cactacgttt ggcaaccacc
    32461 acttcaccgt cacccactga aagcactggc gtaccttgtg gcatggcaaa atcaacacct
    32521 ctgtgtggcg caacgcgacc ggtcaccgga ttagtacgac gcgggttaaa gttagatgag
    32581 atacggaact gtttcgccgt cgggaatcgc aagaatcctt tcgccagacc agtaccgtta
    32641 cgatcataga atttgccatc ttcagcgcgg attgcataat aatctttacc ttctgaacgc
    32701 aaacgtacgc ccagcagctg gctttgctca cgtttaccat caagcatttc tcgtgacatt
    32761 aacaccgcaa attcatcgcc ttttttcagt ttgcggaaat ccatttgcca ctgcatggct
    32821 ttaatcactg cgctcacttc ggcgctggtt aaaccggcgt ttctggcgct ggcaacaaag
    32881 cttcccccga cggtaccttt cagcagattg ttgacccact ctccttgctg catttcgctg
    32941 gtcattttaa aaccgttagc ggcagtacgg tcataggttc gggtttcacg acgagacact
    33001 tcccaggtga ggcgctgcag ttcgccgtcc gcggttaatg tccaggagag ttgttgaccg
    33061 attttcaggt tacgcaattc tttgtcggca gcagccagtt gggtgatatc acccatatca
    33121 ataccatact gattgagaat gctgcttagc gtatcgccag tggaaacaac atattcatgc
    33181 acgcccgctt caccggcgat tttgtcatcc agttcgtcct ggggaatggc ttcatcttct
    33241 tgtgcagctt gatcaatcgg ctcactggct tcaggtaaga gcgaacgaat ttcgttctgt
    33301 tccagctcaa tggttttgac aattggcgtg gcatcacggt gataaacata gggccgccag
    33361 acagcgacgg ccagagtaag aacggtgagc gaccccaaca taacgcggtg tggtcgcggt
    33421 aaattattaa acgccagggc gacagagcgg gctatctgtt gcacgtaatc acttcctcat
    33481 taatctcctt tcaggcagct cgcatactgg ttggctaatt gattcaggaa ttctgaatag
    33541 cttgttttac ccagtttgat attcgtcccc aggggatcca acgttcccat acgaacggat
    33601 gtccctcgtg cgacgctctc aacgaccgct ggcctgaact gtggctcagc aaaaacgcag
    33661 gttgcttttt gctcaaccaa ctgtgttctt atttcatgta aacgctgcgc gccaggttga
    33721 atctcagggt taacggtaaa atgaccaagc ggtgtcagtc cgaactgttt ttcgaagtag
    33781 ccgtaagcat cgtgaaaaac gaaataacct ttccccttga gcggcgcgag ctcgttacca
    33841 acctgcgttt cggttgaggc taattgtgcc tcaaaatcct tcaggttggc gtcaagtttg
    33901 gctcgacttt gcggcataag ttccactaat tttccatgga ttgcaaccgc tgtagcccgc
    33961 gctatctctg gggaaagcca aagatgcatg ttgaaatcgc cgtgatggtg atcttcgtca
    34021 cttttttccg cgtgatcgtg atcatcatca tcgtcgtgaa tacttttcat cagcagcggt
    34081 ttcacatctt ctagctgcgc aatcgttacc tgtttcgctt ctggtaattt acttaccggt
    34141 ttttgcatga acgcttccat ctccgggcca acccaaacga ctaagtccgc gttctgtaag
    34201 cgttttacat ccgatggacg cagtgaataa tcatgttctg aagcgccgtc aggcagtaaa
    34261 acctgcgttt ctgttacccc atcagcaatg gcagaagcga tgaacccaac gggtttaagc
    34321 gaagcgacaa cggcggcatc tgcggcctgt gttgcaccgc cccagagagc ggcggataat
    34381 gctgcgaaaa gaagcgtttt tttatgtaac ataatgcgac caatcatcgt aatgaatatg
    34441 agaagtgtga tattataaca tttcatgact actgcaagac taaaattaac atgacaagtc
    34501 tggtttccct ggaaaatgtc tcggtttctt ttggccaacg ccgcgtcctc tctgatgtgt
    34561 cgctggaact taaacctgga aaaattttga ctttacttgg gccaaacggc gcaggtaagt
    34621 cgacactggt acgggtagtg ctcggactgg taacacccga tgaaggggtt atcaagcgca
    34681 acggaaaact gcgcatcggc tatgtaccgc agaagctgta tctcgacacc acgttgccac
    34741 tgaccgtaaa ccgtttttta cgcttacgcc ctggcacaca taaagaagat attttgcctg
    34801 cactgaaacg tgtccaggcc gggcatctga ttaacgcacc gatgcaaaag ctctcgggtg
    34861 gcgaaacgca gcgtgtactg ttagcgcgag cattgttaaa tcgaccgcaa ttattagtgc
    34921 tggatgaacc cactcagggc gtggatgtga atggtcaggt ggcgttatat gaccttattg
    34981 accaactgcg tcgtgaactg gattgtggcg ttttaatggt atctcacgat ctgcatctgg
    35041 taatggcaaa aaccgatgaa gtgctttgcc tgaatcacca catttgttgt tccggcacac
    35101 cggaagttgt ttccctgcat ccggagttta tttctatgtt tggtcctcgt ggtgctgaac
    35161 aactgggtat ctatcgccat catcataatc atcgtcacga tttacaggga cgaattgttt
    35221 tgcgtcgggg aaatgatcgc tcatgattga attattattt cccggttggt tagccgggat
    35281 catgctcgcc tgtgccgcgg gtccgctggg ttcgtttgta gtctggcgtc gtatgtctta
    35341 tttcggtgat acgctggctc atgcctcatt acttggcgtc gcgtttggtt tgttgctgga
    35401 cgtgaatcca ttctatgcgg tgattgcagt tacgctgctg ctggcgggcg gtctggtatg
    35461 gctcgagaag cgtccacagc tggcgatcga cacgttatta gggattatgg cgcacagtgc
    35521 cctgtcgctg ggcctggtgg tcgttagtct gatgtctaat attcgtgttg atttgatggc
    35581 ttacctgttc ggtgatttac tggcagtgac gccagaagat ctcatctcta ttgcgattgg
    35641 cgtggtcatc gtggtggcta ttttgttctg gcaatggcgc aatttgctgt cgatgacgat
    35701 tagcccggat ctggcgtttg ttgatggtgt gaaattacag cgcgtgaaat tgttgttgat
    35761 gctggtgacg gcattgacga ttggtgtagc gatgaaattc gtcggcgcgt tgattattac
    35821 ttcactgctg attattcctg ctgctactgc acgtcgcttt gcccgcacgc cggaacagat
    35881 ggctggtgtc gctgttttgg tggggatggt ggcagtgact ggcggtttaa ccttttccgc
    35941 attttacgat acacctgcag gcccgtcggt ggtgctatgc gcggcactgt tatttattat
    36001 cagtatgatg aaaaaacagg ccagctaatc tttcgctgaa catatttgtc ggatgcggcg
    36061 cgagcgcctt atccgacctt gtattatccc tccagtgcag agaaaatcgg tcagttttct
    36121 ctgcctgcag tccgcatgcc ttatccgacc tacggttcgc tatctctggt aggcctgata
    36181 agacgaacat gaactgaacc ccaaaacctg gacagtttca gttaaccggc tttttgatac
    36241 tcagtccggt attctaccgg gctgagtccc ccaagactga gttttattct tttttgattc
    36301 cagtaagtta tgtattcgtt aacggcattt tccagctctt ctacatttct gtagtcccgg
    36361 cgatagtaca tttcttcttt caggtgacca aagaagttct ccattaccgc attatcaagg
    36421 cagttgccct tgcgcgacat gctctgcact aaccctctgt ccgccagggc ggactgataa
    36481 ctttttatcc ggtaatgcca tccttgatca ctgtggagca gcggtttctc cccttcagca
    36541 aggctttcca gccccttatt taacatccgc ttcaccagct cctctgtagg cctgcaggcc
    36601 gtttcccacg ccacgatttc cccgttaaac aaatcaagta tcggggacag gtacagtttt
    36661 tgtccgccag ccctgaattc ggtgatatct gtcacccatt tctcacaggg agctgccgct
    36721 ttgaacagtc gctgaaggat attttctgca gcgagtccca tatttccccg gtaagaccgg
    36781 tatttcttca gacgcacagg agacttcagg ccgagctgtt gcatcagttt tcgcaccgtt
    36841 ttgccactga acttaagacc tcttttctga agctcacaat ggatccgccg gtacccataa
    36901 catccccggt gttcatggaa gatggaggct atgagctgtt tcacatcagc atacttgtcc
    36961 ttagccttct gcagtgacag ctggtagtac agcgtgcttc tggccagccc tgcggccttt
    37021 aacaggtcag actggcagtg tccgtacctc aggctctgga tgactttcgt tttttctcca
    37081 gctcctggcg ctttttttcc agaagatgct cctgcatggc tttcagatat gcattttctg
    37141 cgcgaagata acgcagctca ttcttcatct cttccggcgt catgtttgcc tcgttttcat
    37201 gggtatcatc agaacgggtc atgcttttct ccttcctgcg cctgataaag gcctcattac
    37261 cagatttgcg gtagcctttt atccagtttt taaccgtgtt gtggctggga atgttaaacc
    37321 gggctgctac ccggggtaga gactcagaat gcccgagagc ataaagaacc acgcaaagtt
    37381 tatcttcagg agaatagcta cgcttgtgtc tgcaatccag tgcccgagga ccgtgaagaa
    37441 gaaaaagatt aatccagtgt gagaggctgg tgtgagaaag ttgaaagagt tttgccgtag
    37501 aaatgatgcc tgcatgacca gccaggtaat gatttacagc ggcaagtttt acttcaaatg
    37561 aatgtttcat aaactgcacc ttcagatgtc agattatgtc caacatttgg ggtgcagttc
    37621 aacagcgtcg catcaggcac actgccagtg tcggatgcgg cacgagcgac caatccgact
    37681 tacgacattt ctggcggcgt tatgccaaag tgattccacg cccgcgtcgt cgccatacgc
    37741 ccacgcggtg tacgctgcaa aaagccttgc tgaatcaaat aaggttccag tacatcctca
    37801 atggtttcac gttcttcgcc aatggctgcc gccaggttat ccagacctac cggcccgcca
    37861 aagaacttat cgattaccgc cagcaacaat ttgcggtcca tataatcgaa accttcagca
    37921 tcgacattca acatatccag cgcctgagca gcgatatctg ccgagatggt gccatcgtgc
    37981 ttcacttcgg cgaaatcacg cactcgacgc agcagacggt tggcaatacg tggcgtaccg
    38041 cgcgcacgac gagcaacttc cagcgcgccg tcatcactca tctcaagccc cataaagcgt
    38101 gcgctgcgac tgacgatata ttgcagatcc ggcacctgat aaaactccag acgttgcaca
    38161 ataccaaaac gatcgcgcaa cggtgatgtc agcgaacctg cgcgcgtggt tgcaccaatc
    38221 agggtaaacg gcggcaaatc aattttaatg gagcgtgccg ccggaccttc accaatcatg
    38281 atatccagtt ggtaatcttc cattgccgga tacaacacct cttccaccac tggtgaaaga
    38341 cggtggatct catcaataaa cagtacatcg tgtggttcaa ggttagtgag cattgctgcc
    38401 agatcgcccg ccttttccag caccggacca gaagtcgtgc gtaaattaac gcccatttca
    38461 ttggcgacaa tattggcaag cgtagtttta cccaaccccg gaggaccaaa aatcaataga
    38521 tgatcgaggg catcgccgcg cagtttcgct gctttgatga aaatctccat ctgcgaacga
    38581 acctgcggct gaccaacata ctcttccagt aatttagggc gaatggcgcg atctgccaca
    38641 tcttccggca aagtggtacc ggcagaaatc agacggtctg cttcaatcat cctttacctc
    38701 ataacgcggc gcgtagggct tcgcgaatta atgtttcact gctggcgtca gggcgagcga
    38761 ttttgctcac catgcggctt gcttcttgtg gtttatagcc cagtgccacc agcgcagcaa
    38821 ccgcttcctg ttcagcatcg tcggtcgccg ggctggcagg agacgtgagt accaggtcgg
    38881 cggctggcgt aaagagatcg ccatgcaaac ctttaaatcg gtctttcatt tcgacaatca
    38941 aacgttcggc ggtttttttg ccaatacccg gcagtttcac cagtgccccc acttcttcac
    39001 gctcaacggc attaacgaac tgctgcgctg acattccgga gaggatcgcc agcgccaact
    39061 tcgggccgac gccgttggtt ttgatcaact ctttgaacaa cgtgcgctct tgtttattgt
    39121 taaaaccgta cagcagttgc gcgtcttcac gcaccacaaa gtgggtgaaa acgatcgctt
    39181 cctgacccgc ttcagggagt tcataaaaac aggtcatcgg catatgcact tcatagccta
    39241 cgccgcccac ttcaattaac accagcgggg gttgtttttc aatgatgatg cctctgagtc
    39301 tgcctatcac atgacgctcc tgcgtaatga atcaaagata atgctgtatg ataaaaaaat
    39361 gctggataga tatccagcga aggatgaaga aaacttgcga ggtgtctcga tgatctgaaa
    39421 aatg
//
LOCUS       DAHHTG010000032        39377 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.032, whole genome shotgun sequence.
ACCESSION   DAHHTG010000032 DAHHTG010000000
VERSION     DAHHTG010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 39377)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39377)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39377
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.032"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            complement(<1..1235)
                     /gene="amiC"
                     /locus_tag="NBI74_001633"
     CDS             complement(<1..1235)
                     /gene="amiC"
                     /locus_tag="NBI74_001633"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiC"
                     /protein_id="HCD1931992.1"
                     /translation="MSGSNTAISRRRLLQGAGAMWLLSVSQVSLAAVSQVVAVRVWPA
                     SSYTRVTVESNRQLKYKQFALSNPERVVVDIEDVNLNSVLKGMAAQIRADDPFIKSAR
                     VGQFDPQTVRMVFELKQNVKPQLFALAPVAGFKERLVMDLYPANAQDMQDPLLALLED
                     YNKGDLEKQVPPAQSGPQPGKAGRDRPIVIMLDPGHGGEDSGAVGKYKTREKDVVLQI
                     ARRLRSLIEKEGNMKVYMTRNEDIFIPLQVRVAKAQKQRADLFVSIHADAFTSRQPSG
                     SSVFALSTKGATSTAAKYLAQTQNASDLIGGVSKSGDRYVDHTMFDMVQSLTIADSLK
                     FGKAVLNKLGKINKLHKNQVEQAGFAVLKAPDIPSILVETAFISNVEEERKLKTATFQ
                     QEVAESILAGIKAYFADGAT"
     gene            1467..2798
                     /gene="argA"
                     /locus_tag="NBI74_001634"
     CDS             1467..2798
                     /gene="argA"
                     /locus_tag="NBI74_001634"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HCD1931993.1"
                     /translation="MVKERKTELVEGFRHSVPYINTHRGKTFVIMLGGEAIEHENFSS
                     IVNDIGLLHSLGIRLVVVYGARPQIDANLAAHHHEPLYHKNIRVTDAKTLELVKQAAG
                     TLQLDITARLSMSLNNTPLQGAHINVVSGNFIIAQPLGVDDGVDYCHSGRIRRIDEDA
                     IHRQLDSGAIVLMGPVAVSVTGESFNLTSEEIATQLAIKLKAEKMIGFCSSQGVTNDD
                     GDIVSELFPNEAQARVEAQEEKGDYNSGTVRFLRGAVKACRSGVRRCHLISYQEDGAL
                     LQELFSRDGIGTQIVMESAEQIRRATINDIGGILELIRPLEQQGILVRRSREQLEMEI
                     DKFTIIQRDNTTIACAALYPFPEEKIGEMACVAVHPDYRSSSRGEVLLERIAAQAKQS
                     GLSKLFVLTTRSIHWFQERGFTPVDIDLLPESKKQLYNYQRKSKVLMADLG"
     gene            complement(2880..4706)
                     /gene="recD"
                     /locus_tag="NBI74_001635"
     CDS             complement(2880..4706)
                     /gene="recD"
                     /locus_tag="NBI74_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000775924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit alpha"
                     /protein_id="HCD1931994.1"
                     /translation="MKLQKQLLEAVEHKQLRPLDVQFALTVAGDEHPAVTLAAALLSH
                     DAGEGHVCLPLSRLENNEASHPLLATCVSEIGELQNWEECLLASQAVSRGDEPTPMIL
                     CGDRLYLNRMWCNERTVARFFNEVNHAIEVDEALLAQTLDKLFPVSDEINWQKVAAAV
                     ALTRRISVISGGPGTGKTTTVAKLLAALIQMADGERCRIRLAAPTGKAAARLTESLGK
                     ALRQLPLTAEQKKRIPEDASTLHRLLGAQPGSQRLRHHAGNPLHLDVLVVDEASMIDL
                     PMMSRLIDALPDHARVIFLGDRDQLASVEAGAVLGDICAYANAGFTAERAGQLSRLTG
                     SHVPAGTGTEAASLRDSLCLLQKSYRFGSDSGIGQLAAAINRGDKTAVKTVFQQDFTD
                     IEKRLLQSGEDYIAMLEEALAGYGRYLDLLQARAEPDLIIQAFNEYQLLCALREGPFG
                     VAGLNERIEQFMQQKRKIHRHPHSRWYEGRPVMIARNDSALGLFNGDIGIALDRGQGT
                     RVWFAMPDGNIKSVQPSRLPEHETTWAMTVHKSQGSEFDHAALILPSQRTPVVTRELV
                     YTAVTRARRRLSLYADERILSAAIATRTERRSGLAALFSSRG"
     gene            complement(4706..8248)
                     /gene="recB"
                     /locus_tag="NBI74_001636"
     CDS             complement(4706..8248)
                     /gene="recB"
                     /locus_tag="NBI74_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit beta"
                     /protein_id="HCD1931995.1"
                     /translation="MSDVAETLDPLRLPLQGERLIEASAGTGKTFTIAALYLRLLLGL
                     GGSAAFPRPLTVEELLVVTFTEAATAELRGRIRSNIHELRIACLRETTDNPLYKRLLE
                     EIDDKAQAAQWLLLAERQMDEAAVFTIHGFCQRMLNLNAFESGMLFEQQLIEDESLLR
                     YQACADFWRRHCYPLPREIAQVVFETWKGPQALLRDINRYLQGEAPVIKAPPPDDETL
                     ASRHAQIVARIDAVKQQWRDAVGELDALIESSGIDRRKFNRSNQAKWIEKISAWAEEE
                     TNSYQLPESLEKFSQRFLEDRTKAGGETPRHPLFEAIDQLLAEPLSIRDLVITRALAE
                     IRETVAREKRRRGELGFDDMLSRLDSALRSESGEVLAAAIRTRFPVAMIDEFQDTDPQ
                     QYRIFRRIWHHQPETALLLIGDPKQAIYAFRGADIFTYMKARSEVHAHYTLDTNWRSA
                     PGMVNSVNKLFSQTDDAFMFREIPFIPVKSAGKNQALRFVFKGEMQPAMKMWLMEGES
                     CGVGDYQSTMAQVCAAQIRDWLQAGQRGEALLMNGDDARPVRASDISVLVRSRQEAAQ
                     VRDALTLLEIPSVYLSNRDSVFETLEAQEMLWLLQAVMTPERENTLRSALATSMMGLN
                     ALDIETLNNDEHAWDAVVEEFDGYRQIWRKRGVMPMLRALMSARNIAENLLATAGGER
                     RLTDILHISELLQEAGTQLESEHALVRWLSQHILEPDSNASSQQMRLESDKHLVQIVT
                     IHKSKGLEYPLVWLPFITNFRVQDQAFYHDRHSFEAVLDLNAAPESVDLAEAERLAED
                     LRLLYVALTRSVWHCSLGVAPLVRRRGDKKGDTDVHQSALGRLLQKGEPQDVAGLRTC
                     IEALCDDDIAWQTAQIGDNQPWQVNDALTAELNARTLQRLPGDNWRVTSYSGLQQRGH
                     GIAQDLMPRLDVDAAGVVSVVEVPTLTPHQFPRGASPGTFLHSLFEDLDFTQPVDPNW
                     VQEKLELGGFEPQWEPVLTEWITAVLLAPLNETGISLSQLSDRDKQVEMEFYLPISEP
                     LIASQLDALIRQFDPLSAGCPPLEFMQVRGMLKGFIDLVFRHEGRYYLLDYKSNWLGE
                     DSSAYTQQAMAAAMQAHRYDLQYQLYTLALHRYLRHRIADYDYERHFGGVIYLFLRGV
                     DKEHPQQGIYATRPNAGLIDLMDEMFAGMTLEEA"
     gene            complement(8241..11129)
                     /gene="ptrA"
                     /locus_tag="NBI74_001637"
     CDS             complement(8241..11129)
                     /gene="ptrA"
                     /locus_tag="NBI74_001637"
                     /EC_number="3.4.24.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin"
                     /protein_id="HCD1931996.1"
                     /translation="MPRSIWFKALLLFVALWAPLSQAETGWQPIQETIRKSDKDNRQY
                     QAIRLDNGMVVLLVSDPQAVKSLSALVVPVGSLEDPEAYQGLAHYLEHMSLMGSKKYP
                     QADSLAEYLKMHGGSHNASTAPYRTAFYLEVENDALPGAVDRLADAIAEPLLDKKYAE
                     RERNAVNAELTMARTRDGMRMAQVSAETINPAHPGSKFSGGNLETLSDKPGNPVQQAL
                     KDFHEKYYSANLMKAVIYSNKPLPELAKMAADTFGRVPNKESKKPEITVPVVTDAQKG
                     IIIHYVPALPRKVLRVEFRIDNNSAKFRSKTDELITYLIGNRSPGTLSDWLQKQGLVE
                     GISANSDPIVNGNSGVLAISASLTDKGLANRDQVVAAIFSYLNLLREKGIDKQYFDEL
                     ANVLDIDFRYPSITRDMDYVEWLADTMIRVPVEHTLDAVNIADRYDAKAVKERLAMMT
                     PQNARIWYISPKEPHNKTAYFVDAPYQVDKISAQTFADWQKKAANIALSLPELNPYIP
                     DDFSLIKSEKKYDHPELIVDESNLRVVYAPSRYFASEPKADVSLILRNPKAMDSARNQ
                     VMFALNDYLAGLALDQLSNQASVGGISFSTNANNGLMVNANGYTQRLPQLFQALLEGY
                     FSYTATEDQLEQAKSWYNQMMDSAEKGKAFEQAIMPAQMLSQVPYFSRDERRKILPSI
                     TLKEVLAYRDALKSGARPEFMVIGNMTEAQAITLARHVQKQLGADGSEWCRNKDVVVD
                     KKQSVIFEKAGNSADSALAAVFVPTGYDEYTSSAYSSLLGQIVQPWFYNQLRTEEQLG
                     YAVFAFPMSVGRQWGMGFLLQSNDKQPSFLWERYKAFFPTAEAKLRAMKPEEFAQIQQ
                     AVITQMLQAPQTLGEEASKLSKDFDRGNMRFDSRDKIVAQIKLLTPQKIADFFHQAVV
                     EPQGMAILSQISGSQNGKAEYVHPEGWKVWENVSALQQTMPLMSEKNE"
     gene            complement(11305..14673)
                     /gene="recC"
                     /locus_tag="NBI74_001638"
     CDS             complement(11305..14673)
                     /gene="recC"
                     /locus_tag="NBI74_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HCD1931997.1"
                     /translation="MLRVYHSNRLDVLEALMEFIVERERLDDPFEPEMILVQSTGMAQ
                     WLQMTLSQKFGIAANIDFPLPASFIWDMFVRVLPEIPKESAFNKQSMSWKLMTLLPQL
                     LEREDFTLLRHYLTDDSDKRKLFQLSSKAADLFDQYLVYRPDWLAQWETGHLVEGLGE
                     AQAWQAPLWKALVEYTDELGQPRWHRANLYQRFIETLESATTCPPGLPSRVFICGISA
                     LPPVYLQALQALGKHIEIHLLFTNPCRYYWGDIKDPAYLAKLLTRQRRHSFEDRELPL
                     FRDSENAGQLFNSDGEQDVGNPLLASWGKLGRDYIYLLSDLESSQELDAFVDVTPDNL
                     LHNIQSDILELENRAVAGVNIEEFSRSDNKRPLDPLDSSITFHVCHSPQREVEVLHDR
                     LLAMLEEDPTLTPRDIIVMVADIDSYSPFIQAVFGSAPADRYLPYAISDRRARQSHPV
                     LEAFISLLSLPDSRFVSEDVLALLDVPVLAARFDITEEGLRYLRQWVNESGIRWGIDD
                     DNVRELELPATGQHTWRFGLTRMLLGYAMESAQGEWQSVLPYDESSGLIAELVGHLAS
                     LLMQLNIWRRGLAQERPLEEWLPVCRDMLNAFFLPDAETEAAMTLIEQQWQAIIAEGL
                     GAQYGDAVPLSLLRDELAQRLDQERISQRFLAGPVNICTLMPMRSIPFKVVCLLGMND
                     GVYPRQLAPLGFDLMSQKPKRGDRSRRDDDRYLFLEALISAQQKLYISYIGRSIQDNS
                     ERFPSVLVQELIDYIGQSHYLPGDEALNCDESEARVKAHLTCLHTRMPFDPQNYQPGE
                     RQSYAREWLPAASQAGKAHSEFVQPLPFTLPETVPLETLQRFWAHPVRAFFQMRLQVN
                     FRTEDSEIPDTEPFILEGLSRYQINQQLLNALVEQDDAERLFRRFRAAGDLPYGAFGE
                     IFWETQCQEMQQLADRVIACRQPGQSMEIDLACNGVQITGWLPQVQPDGLLRWRPSLL
                     SVAQGMQLWLEHLVYCASGGNGESRLFLRKDGEWRFPPLAAEQALHYLSQLIEGYREG
                     MSAPLLVLPESGGAWLKTCYDAQNDAMLDDDSTLQKARTKFLQAYEGNMMVRGEGDDI
                     WYQRLWRQLTPETMEAIVEQSQRFLLPLFRFNQS"
     gene            complement(14686..15009)
                     /locus_tag="NBI74_001639"
     CDS             complement(14686..15009)
                     /locus_tag="NBI74_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HCD1931998.1"
                     /translation="MSASLRNQQGFSLPEVMLAMVLMVMIVTALSGFQRTLMNSLASR
                     NQYQQLWRHGWQQTQLRAISPPANWQVNRMQTSQAGCVSISVTLVSPGGREGEMTRLH
                     CPNRQ"
     gene            complement(14994..15401)
                     /locus_tag="NBI74_001640"
     CDS             complement(14994..15401)
                     /locus_tag="NBI74_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311708.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2509 family protein"
                     /protein_id="HCD1931999.1"
                     /translation="MNREKGVSSLALVLMLLILGSLLLQGMSQQDRSFASRVSMESQS
                     LRRQAIVQSALEWGKMHSWQTLPAVQCLLYAATGARVCLRLLADNEALLIAGYEGVSL
                     WRTGEVIDGNIVFSPRGWSDFCPLKERALCQLP"
     gene            complement(15398..15961)
                     /locus_tag="NBI74_001641"
     CDS             complement(15398..15961)
                     /locus_tag="NBI74_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HCD1932000.1"
                     /translation="MPVKEQGFSLLEVLIAMAISSVLLLGAARFLPALQRESLTNTRK
                     LALEDEIWLRVFTVAKHLQRAGYCHGSCTGEGLEIVGQGDCVIVQWDANSNGIWDREP
                     VKESDQIGFRLKEHVLETLRGATSCEGKGWDKVTNPDAIIIDTFQVVRQDVSGFSPVL
                     TVNMHAASKADPQTVVDASYSVTGSNL"
     gene            complement(15952..16422)
                     /locus_tag="NBI74_001642"
     CDS             complement(15952..16422)
                     /locus_tag="NBI74_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HCD1932001.1"
                     /translation="MKKQRGYTLIETLVAMLILVMLSASGLYGWQYWQQSQRLWQTAS
                     QARDYLLYLREDANWHNRDHSISLIREGTLWCLVSSVAGANTCHGSSPLVFVPRWPEV
                     EMSDLTPSLAFFGLRNTAWAGHIRFKNSTGEWWLVVSPWGRLRLCQQGETEGCL"
     gene            complement(16607..17401)
                     /gene="thyA"
                     /locus_tag="NBI74_001643"
     CDS             complement(16607..17401)
                     /gene="thyA"
                     /locus_tag="NBI74_001643"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HCD1932002.1"
                     /translation="MKQYLELMQKVLDEGTQKNDRTGTGTLSIFGHQMRFNLQDGFPL
                     VTTKRCHLRSIIHELLWFLQGDTNIAYLHENNVTIWDEWADENGDLGPVYGKQWRAWP
                     TPDGRHIDQITTVLNQLKNDPDSRRIIVSAWNVGELDKMALAPCHAFFQFYVADGKLS
                     CQLYQRSCDVFLGLPFNIASYALLVHMMAQQCDLEVGDFVWTGGDTHLYSNHMDQTHL
                     QLSREPRPLPKLIIKRKPESIFDYRFEDFEIEGYDPHPGIKAPVAI"
     gene            complement(17408..18283)
                     /gene="lgt"
                     /locus_tag="NBI74_001644"
     CDS             complement(17408..18283)
                     /gene="lgt"
                     /locus_tag="NBI74_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HCD1932003.1"
                     /translation="MTSSYLHFPEFDPVIFSIGPVALHWYGLMYLVGFIFAMWLATRR
                     ANRPGSGWTKNEVENLLYAGFLGVFLGGRIGYVLFYNFPQFMADPLYLFRVWDGGMSF
                     HGGLIGVIVVMIIFARRTKRSFFQVSDFIAPLIPFGLGAGRLGNFINGELWGRVDPNF
                     PFAMLFPGSRTEDILLLQTNPQWQSIFDTYGVLPRHPSQLYELLLEGVVLFIILNLYI
                     RKPRPMGAVSGLFLIGYGAFRIIVEFFRQPDAQFTGAWVQYISMGQILSIPMIVAGVI
                     MMVWAYRRSPQQHVS"
     gene            complement(18434..20680)
                     /gene="ptsP"
                     /locus_tag="NBI74_001645"
     CDS             complement(18434..20680)
                     /gene="ptsP"
                     /locus_tag="NBI74_001645"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HCD1932004.1"
                     /translation="MLTRLREIVERVASAPRLNEALNILVTDICLAMDTEVCSVYLAD
                     HDRRCYYLMATRGLKKPRGRTVTLAFDEGIVGLVGRLAEPINLADAQKHPSFKYIPSV
                     KEERFRAFLGVPIIQRRQLLGVLVVQQRELRQYDESEESFLVTLATQMAAILSQSQLT
                     ALFGQYRQTRIRALPAAPGVAIAEGWQDATLPLMEQVYQASTLDPALERERLTGALEE
                     AANEFRRYSKRFAAGAQKETAAIFDLYSHLLSDTRLRRELFAEVDKGSVAEWAVKTVI
                     EKFAEQFAALSDNYLKERAGDLRALGQRLLFHLDDANQGPNAWPERFILVADELSATT
                     LAELPQDRLVGVVVRDGAANSHAAIMVRALGIPTVMGADIQPSVLHRRTLIVDGYRGE
                     LLVDPEPVLLQEYQRLISEEIELSRLAEDDVNLPAQLKSGERIKVMLNAGLSPEHEEK
                     LGSRIDGIGLYRTEIPFMLQSGFPSEEEQVAQYQGMLQMFNDKPVTLRTLDVGADKQL
                     PYMPISEENPCLGWRGIRITLDQPEIFLIQVRAMLRANAATGNLNILLPMVTSLDEVD
                     EARRLIERAGREVEEMIGYEIPKPRIGIMLEVPSMVFMLPHLAKRVDFISVGTNDLTQ
                     YILAVDRNNTRVANIYDSLHPAMLRALAMIAREAEIHGIDLRLCGEMAGDPMCVAILI
                     GLGYRHLSMNGRSVARVKYLLRRIDFAEAENLAQRSLEAQLATEVRHQVAAFMERRGM
                     GGLIRGGL"
     gene            complement(20693..21223)
                     /gene="rppH"
                     /locus_tag="NBI74_001646"
     CDS             complement(20693..21223)
                     /gene="rppH"
                     /locus_tag="NBI74_001646"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001655775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HCD1932005.1"
                     /translation="MIDDDGYRPNVGIVICNRQGQVMWARRFGQHSWQFPQGGINPGE
                     SAEQAMYRELFEEVGLSRKDVRILASTRNWLRYKLPKRLVRWDTKPVCIGQKQKWFLL
                     QLVSGDAEINMQTSSTPEFDGWRWVSYWYPVRQVVSFKRDVYRRVMKEFASVVMSLQE
                     NTPKPQNASAYRRKRG"
     gene            21908..22597
                     /gene="mutH"
                     /locus_tag="NBI74_001647"
     CDS             21908..22597
                     /gene="mutH"
                     /locus_tag="NBI74_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutH"
                     /protein_id="HCD1932006.1"
                     /translation="MSQPRPLLSPPETEEQLLAQAQQLSGYTLGELAALAGLVTPENL
                     KRDKGWIGVLLEIWLGASAGSKPEQDFAALGVELKTIPVDSLGRPLETTFVCVAPLTG
                     NSGVTWETSHVRHKLKRVLWIPVEGERSIPLAKRRVGSPLLWSPNEEEDRQLREDWEE
                     LMDMIVLGQIERITARHGEYLQIRPKAANAKALTEAIGARGERILTLPRGFYLKKNFT
                     SALLARHFLIQ"
     gene            22666..23379
                     /locus_tag="NBI74_001648"
     CDS             22666..23379
                     /locus_tag="NBI74_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HCD1932007.1"
                     /translation="MLFAWITDPNAWLALGTLTLLEIVLGIDNIIFLSLVVAKLPTAQ
                     RAHARRLGLAGAMVMRLALLASIAWVTRLTNPLFTIFSQEISARDLILLLGGLFLIWK
                     ASKEIHESIEGEEEGLKTRVSSFLGAIVQIMLLDIIFSLDSVITAVGLSDHLFIMMAA
                     VVIAVGVMMFAARSIGDFVERHPSVKMLALSFLILVGFTLILESFDIHVPKGYIYFAM
                     FFSIAVESLNLIRNKKNPL"
     gene            23517..23735
                     /gene="ygdR"
                     /locus_tag="NBI74_001649"
     CDS             23517..23735
                     /gene="ygdR"
                     /locus_tag="NBI74_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YgdR"
                     /protein_id="HCD1932008.1"
                     /translation="MKKWAVIISAVGLAFAVSGCSSDYVMATKDGRMILTDGKPEIDD
                     DTGLVSYHDQQGNAMQINRDDVSQIIER"
     gene            23843..24883
                     /locus_tag="NBI74_001650"
     CDS             23843..24883
                     /locus_tag="NBI74_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(H)-dependent aldo-keto reductase"
                     /protein_id="HCD1932009.1"
                     /translation="MQYHRIPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGI
                     NLIDVAEMYPVPPRPETQGLTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPD
                     QALDRKNIREALHDSLKRLQTDYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDT
                     LDALAEYQRAGKIRYIGVSNETAFGVMRYLHLADKHDLPRIVTIQNPYSLLNRSFEVG
                     LAEVSQYEGVELLAYSCLGFGTLTGKYLNGAKPAGARNTLFSRFTRYSGEQTQKAVAA
                     YVDIARRHSLDPAQMALAFVRRQPFVASTLLGATTMEQLKTNVESLHLELSEDVLAEI
                     EAVHQVYTYPAP"
     gene            complement(24915..26108)
                     /gene="lplT"
                     /locus_tag="NBI74_001651"
     CDS             complement(24915..26108)
                     /gene="lplT"
                     /locus_tag="NBI74_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid transporter LplT"
                     /protein_id="HCD1932010.1"
                     /translation="MSESVHTNTSLWSKGMKAVIVAQFLSAFGDNALLFATLALLKAQ
                     FYPEWSQPILQMVFVGAYILFAPFVGQVADSFAKGRVMMFANGLKLLGAASICFGINP
                     FLGYTLVGVGAAAYSPAKYGILGELTTGSKLVKANGLMEASTIAAILLGSVAGGVLAD
                     WHVLIALAACALAYGGAVIANIYIPKLAAARPGQSWNLISMTRSFLNACTSLWRNGET
                     RFSLVGTSLFWGAGVTLRFLLVLWVPVALGITDNATPTYLNAMVAIGIVVGAGAAAKL
                     VTLETVSRCMPAGILIGVVVLIFSLQHELLPAYALLMLIGVLGGFFVVPLNALLQERG
                     KKSVGAGNAIAVQNLGENSAMLLMLGIYSLAVMVGIPVVPIGIGFGALFALAITALWI
                     WQRRH"
     gene            complement(26101..28260)
                     /gene="aas"
                     /locus_tag="NBI74_001652"
     CDS             complement(26101..28260)
                     /gene="aas"
                     /locus_tag="NBI74_001652"
                     /EC_number="2.3.1.40"
                     /EC_number="6.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acyl-ACP--phospholipid
                     O-acyltransferase/long-chain-fatty-acid--ACP ligase"
                     /protein_id="HCD1932011.1"
                     /translation="MLFSFFRNLCRVLYRVRVTGDTKALKGERVLITPNHVSFIDGIL
                     LALFLPVRPVFAVYTSISQQWYMRWLKSFIDFVPLDPTQPMAIKHLVRLVEQGRPVVI
                     FPEGRITTTGSLMKIYDGAGFVAAKSGATVIPVRIEGAELTHFSRLKGLVKRRLFPQI
                     TLHILPPTQVEMPDAPRARDRRKIAGEMLHQIMMEARMAVRPRETLYESLLSAMYRFG
                     AGKKCVEDVNFTPDSYRKLLTKTLFVGRILEKYSVEGERIGLMLPNAGISAAVIFGAI
                     ARRRIPAMMNYTAGVKGLTSAITAAEIKTIFTSRQFLDKGKLWHLPEQLTQVRWVYLE
                     DLKADVTTADKVWIFAHLLMPRLAQLKQQPEEEALILFTSGSEGHPKGVVHSHKSILA
                     NVEQIKTIADFTTNDRFMSALPLFHSFGLTVGLFTPLLTGAEVFLYPSPLHYRIVPEL
                     VYDRSCTVLFGTSTFLGHYARFANPYDFYRLRYVVAGAEKLQESTKQLWQDKFGLRIL
                     EGYGVTECAPVVSINVPMAAKPGTVGRILPGMDARLLSVPGIEEGGRLQLKGPNIMNG
                     YLRVEKPGVLEVPTAENIRGEMERGWYDTGDIVRFDEQGFVQIQGRAKRFAKIAGEMV
                     SLEMVEQLALGVSPDKVHATAIKSDASKGEALVLFTTDNELTRDKLQQYAREHGVPEL
                     AVPRDIRYLKQMPLLGSGKPDFVTLKSWVDEAEQHDE"
     gene            28846..29877
                     /gene="galR"
                     /locus_tag="NBI74_001653"
     CDS             28846..29877
                     /gene="galR"
                     /locus_tag="NBI74_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalR"
                     /protein_id="HCD1932012.1"
                     /translation="MATIKDVARLAGVSVATVSRVINNSPKASEASRLAVHSAMESLS
                     YHPNANARALAQQTTETIGLVVGDVSDPFFGAMVKAVEQVAYHTGNFLLIGNGYHNEQ
                     KERQAIEQLIRHRCAALVVHAKMIPDADLASLMKQMPGMVLINRILPGFENRCIALDD
                     RYGAWLATRHLIQQGHTRIGYLCSNHSISDAEDRLQGYYDALAESGIPANDRLVTFGE
                     PDESGGEQAMTELLGRGRNFTAVACYNDSMAAGAMGVLNDNGIDVPGEISLIGFDDVL
                     VSRYVRPRLTTVRYPIVTMATQAAELALALADNRPLPEITNVFSPTLVRRHSVSTPSL
                     EASHHATSD"
     gene            complement(29884..31146)
                     /gene="lysA"
                     /locus_tag="NBI74_001654"
     CDS             complement(29884..31146)
                     /gene="lysA"
                     /locus_tag="NBI74_001654"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HCD1932013.1"
                     /translation="MPHSLFSTDTDLTAENLLRLPAEFGCPVWVYDAQIIRRQIAALK
                     QFDVVRFAQKACSNIHILRLMREQGVKVDSVSLGEIERALAAGYNPQTHPDDIVFTAD
                     VIDQATLERVSELQIPVNAGSVDMLDQLGQVSPGHRVWLRVNPGFGHGHSQKTNTGGE
                     NSKHGIWYTDLPAALDVIQRHHLQLVGIHMHIGSGVDYAHLEQVCGAMVRQVLEFGQD
                     LQAISAGGGLSIPYQQGEEAVDTEHYYGLWNAAREQIARHLGHPVKLEIEPGRFLVAQ
                     AGVLITQVRSVKQMGSRHFVLVDAGFNDLMRPAMYGSYHHISALAADGRSLEHAPTVE
                     TVVAGPLCESGDVFTQQEGGNVETRALPEVKAGDYLVLHDTGAYGASMSSNYNSRPLL
                     PEVLFDNGQARLIRRRQTIEELLALELL"
     gene            31268..32203
                     /gene="lysR"
                     /locus_tag="NBI74_001655"
     CDS             31268..32203
                     /gene="lysR"
                     /locus_tag="NBI74_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator LysR"
                     /protein_id="HCD1932014.1"
                     /translation="MAAVNLRHIEIFHAVMTAGSLTEAAHLLHTSQPTVSRELARFEK
                     VIGLKLFERIRGRLHPTVQGLRLFEEVQRSWYGLDRIVSAAESLREFRQGELSIACLP
                     VFSQSFLPQLLQPFLARYPDVSLNIVPQESPLLEEWLSAQRHDLGLTETLHTPAGTER
                     TELLSLDEVCVLPPGHPLAVKKVLTPDDFHGENYISLSRTDSYRQLLDQLFTENQVKR
                     RMIVETHSAASVCAMVRAGVGVSVVNPLTALDYAASGLVVRRFSIAVPFTVSLIRPLH
                     RPSSALVQAFSEHLQAGLPKLVTSLDAILSSATTA"
     gene            complement(32190..32882)
                     /gene="ygeA"
                     /locus_tag="NBI74_001656"
     CDS             complement(32190..32882)
                     /gene="ygeA"
                     /locus_tag="NBI74_001656"
                     /EC_number="5.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HCD1932015.1"
                     /translation="MKTIGLLGGMSWESTIPYYRLINEGIKQRLGGLHSAQVLLHSVD
                     FHEIEECQRRGEWDKTGDILAEAALGLQRAGAEGIVLCTNTMHKVADAIESRCSLPFL
                     HIADATGRAITGAGMTRVALLGTRYTMEQDFYRGRLTEQFSINCLIPEADDRAKINQI
                     IFEELCLGQFTEASRAYYAQVIARLAEQGAQGVIFGCTEIGLLVPEERSVLPVFDTAA
                     IHAEDAVAFMLS"
     gene            complement(33011..34429)
                     /gene="araE"
                     /locus_tag="NBI74_001657"
     CDS             complement(33011..34429)
                     /gene="araE"
                     /locus_tag="NBI74_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-proton symporter AraE"
                     /protein_id="HCD1932016.1"
                     /translation="MVTINTESALTPRPLRDTRRMNMFVSVAAAVAGLLFGLDIGVIA
                     GALPFITDHFVLTSRLQEWVVSSMMLGAAIGALFNGWLSFRLGRKYSLMAGAILFVLG
                     SIGSAFATSVEMLIAARVVLGIAVGIASYTAPLYLSEMASENVRGKMISMYQLMVTLG
                     IVLAFLSDTAFSYSGNWRAMLGVLALPAVLLIILVVFLPNSPRWLAEKGRHIEAEEVL
                     RMLRDTSEKAREELNEIRESLKLKQGGWALFKINRNVRRAVFLGMLLQAMQQFTGMNI
                     IMYYAPRIFKMAGFTTTEQQMIATLVVGLTFMFATFIAVFTVDKAGRKPALKIGFSVM
                     ALGTLVLGYCLMQFDNGTASSGLSWLSVGMTMMCIAGYAMSAAPVVWILCSEIQPLKC
                     RDFGITCSTTTNWVSNMIIGATFLTLLDSIGAAGTFWLYTALNIAFVGITFWLIPETK
                     NVTLEHIERKLMAGEKLRNIGV"
     gene            complement(34744..35505)
                     /gene="kduD"
                     /locus_tag="NBI74_001658"
     CDS             complement(34744..35505)
                     /gene="kduD"
                     /locus_tag="NBI74_001658"
                     /EC_number="1.1.1.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-D-gluconate 5-dehydrogenase
                     KduD"
                     /protein_id="HCD1932017.1"
                     /translation="MILSAFSLEGKVAVVTGCDTGLGQGMALGLAQAGCDIVGINIVE
                     PTETIKQVTALGRRFLSLTADLRKIDGIPALLDRAVAEFGHIDILVNNAGLIRREDAL
                     EFSEKDWDDVMNLNIKSVFFMSQAAAKHFIAQGNGGKIINIASMLSFQGGIRVPSYTA
                     SKSGVMGVTRLMANEWAKHNINVNAIAPGYMATNNTQQLRADEQRSAEILDRIPAGRW
                     GLPSDLMGPVVFLASSASDYVNGYTIAVDGGWLAR"
     gene            complement(35535..36371)
                     /gene="kduI"
                     /locus_tag="NBI74_001659"
     CDS             complement(35535..36371)
                     /gene="kduI"
                     /locus_tag="NBI74_001659"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HCD1932018.1"
                     /translation="MDVRQSIHSAHAKTLDTQGLRNEFLVEKVFVADEYTMVYSHIDR
                     IIVGGIMPVTKTVSVGGEVGKQLGVSYFLERRELGVINIGGAGTITVDGQCYEIGHRD
                     ALYVGKGAKEVVFASIDTATPAKFYYNCAPAHTTYPTKKVTPDEVSPVTLGDNLTSNR
                     RTINKYFVPDVLETCQLSMGLTELAPGNLWNTMPCHTHERRMEVYFYFNMDDDACVFH
                     MMGQPQETRHIVMHNEQAVISPSWSIHSGVGTKAYTFIWGMVGENQVFDDMDHVAVKD
                     LR"
     gene            complement(36658..37839)
                     /locus_tag="NBI74_001660"
     CDS             complement(36658..37839)
                     /locus_tag="NBI74_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HCD1932019.1"
                     /translation="MKDVVIVGALRTPIGCFRGALAGHSAVELGSLVVKALIERTGVP
                     AYAVDEVILGQVLTAGAGQNPARQSAIKGGLPNSVSAITINDVCGSGLKALHLATQAI
                     QCGEADIVIAGGQENMSRAPHVLTDSRTGAQLGNSQLVDSLVHDGLWDAFNDYHIGVT
                     AENLAREYGISRQLQDAYALSSQQKARAAIDAGRFKDEIVPVITQSNGQTLVVDTDEQ
                     PRTDTSAEGLARLNPSFDSLGSVTAGNASSINDGAAAVMMMSEAKARALNLPVLARIR
                     AFASVGVDPALMGIAPVYATRRCLERVGWQLADVDLIEANEAFAAQALSVGKMLEWDE
                     RRVNVNGGAIALGHPIGASGCRILVSLVHEMVKRNARKGLATLCIGGGQGVALTIERD
                     E"
     gene            38094..39323
                     /locus_tag="NBI74_001661"
     CDS             38094..39323
                     /locus_tag="NBI74_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease"
                     /protein_id="HCD1932020.1"
                     /translation="MSNIWSKEETLWSFALYGTAVGAGTLFLPIQLGSAGAVVLFITA
                     LVAWPLTYWPHKALCQFILSSKTSAGEGITGAVTHYYGKKIGNLITTLYFIAFFVVVL
                     IYAVAITNSLTEQLAKHMVIDLRIRMLVSLGVVLILNLIFLMGRHATIRVMGFLVFPL
                     IAYFLFLSIYLVGSWQPDLLTTQVEFNQNTLHQIWISIPVMVFAFSHTPIISTFAIDR
                     REKYGEHAMDKCKKIMKVAYLIICISVLFFVFSCLLSIPPSYIEAAKEEGVTILSALS
                     MLPNAPAWLSISGIIVAVVAMSKSFLGTYFGVIEGATEVVKTTLQQVGVKKSRAFNRA
                     LSIMLVSLITFIVCCINPNAISMIYAISGPLIAMILFIMPTLSTYLIPALKPWRSIGN
                     LITLIVGILCVSVMFFS"
BASE COUNT         9754 a        10707 c         9609 g         9307 t
ORIGIN      
        1 gtcgccccat cggcaaaata cgctttaatt cccgcaagaa tagactccgc aacttcctgc
       61 tggaaagtcg ccgttttcag tttacgctct tcctcaacgt tactgataaa cgccgtttcg
      121 accagaatgg agggaatatc gggcgctttc agcacagcaa acccggcctg ttcaacttga
      181 tttttatgca gcttgttgat tttaccgagc ttattcagca ccgctttacc aaacttaagg
      241 ctgtcggcaa tggtcagcga ctgcaccata tcgaacatgg tgtggtcgac atagcggtca
      301 ccgcttttgc ttacgccacc aatcaagtcc gaggcgttct gggtttgtgc cagatatttt
      361 gccgcagtac tggttgcgcc tttggttgag agcgcaaaca ccgaggaacc gctcggctgg
      421 cgactggtaa aggcgtcggc atggatagag acaaacaagt cagcgcgctg tttctgggct
      481 tttgctacgc gcacttgcaa cggaatgaag atgtcttcat tgcgcgtcat gtacaccttc
      541 atattgccct ctttctcgat cagagagcgc agacggcgag ctatttgcaa taccacgtct
      601 ttttcgcgtg ttttgtattt ccccaccgca ccggagtctt cgccaccgtg gccagggtca
      661 agcataatga caatcggacg atcccgtcct gctttacccg gttgtggacc actttgtgct
      721 ggcggcacct gcttttcgag gtcgcctttg ttgtaatcct ccagcagcgc cagcagcggg
      781 tcctgcatat cctgtgcatt ggccggatag aggtccatca ccagacgctc tttaaacccg
      841 gcgaccggcg caagggcaaa cagctgcggt tttacgtttt gctttaattc aaaaaccata
      901 cgtacggttt gcgggtcaaa ttgcccgacg cgcgccgact tgatgaacgg gtcgtcagcg
      961 cggatttgcg ccgccatccc cttgagcacc gagttcaggt ttacatcttc gatatccacc
     1021 accacgcgtt cagggttact caacgcgaac tgcttatatt tcagctgacg atttgattct
     1081 accgtcacgc gggtgtagct ggacgcaggc cagacgcgca ccgccacgac ctggctgacc
     1141 gcagccaggc tgacctgact tacgctcaat agccacatgg cacccgcgcc ttgcagtaaa
     1201 cgacgacggc tgattgcagt gttggatcct gacatgcctc tcccgagcaa aagaaatcta
     1261 agctgtgtaa caagtaaacg accaatttga ccggaaactt tagcgaaaga cgcataatct
     1321 gtcatctata aaacggtaaa cattcttttt atattcacgg tattactgat aaaaaactcg
     1381 ctcccgcata aaatttacac ttgcaccctg cgaaaaaaca gaataaaaat acaataattt
     1441 cgaataatca tgcaaagagg tgtgccgtgg taaaggaacg taaaaccgag ctggtcgagg
     1501 gattccgcca ttcggttccc tatatcaata cccaccgggg aaaaacgttt gtcatcatgc
     1561 tcggcggtga agccattgag catgagaatt tctccagtat cgttaatgat atcgggttgt
     1621 tgcacagcct cggcatccgt ctggtggtgg tctatggcgc acgtccgcag atcgacgcaa
     1681 atctggctgc acatcaccac gaaccgctgt atcacaagaa tatacgtgtg accgatgcca
     1741 aaacactgga actggtgaag caggcagcgg gaacattgca actggatatt actgctcgcc
     1801 tgtcgatgag tctcaataac acaccactgc agggcgcgca tatcaacgtc gtcagtggta
     1861 attttattat tgcccagccg ctgggcgtcg atgacggcgt ggattactgt catagcgggc
     1921 gtatccggcg gattgatgaa gacgcgatcc atcgtcaact ggacagcggt gcaatagtgc
     1981 taatggggcc ggtcgctgtt tcggtcactg gcgagagctt taatctgacc tcggaagaga
     2041 ttgccactca actggccatc aaactgaaag ctgaaaaaat gattggtttt tgctcttccc
     2101 agggcgtcac taatgacgac ggtgatattg tctccgaact tttccctaac gaagcgcaag
     2161 cacgggtaga agcccaggaa gagaaaggcg attacaactc cggtacggtg cgctttttgc
     2221 gtggcgcagt gaaagcctgc cgcagcggcg tgcgtcgctg tcatttaatc agttatcagg
     2281 aagatggcgc gctgttgcaa gagttgttct cacgcgacgg tatcggtacg cagattgtga
     2341 tggaaagcgc cgagcagatt cgtcgcgcaa caatcaacga tattggcggt attctggagt
     2401 tgattcgccc gctggagcaa caaggtattc tggtacgccg ttctcgcgag cagctggaga
     2461 tggaaatcga caaattcacc attattcagc gcgataacac gactattgcc tgcgccgcgc
     2521 tctatccgtt cccggaagag aagattgggg aaatggcctg tgtggcagtt cacccggatt
     2581 accgcagttc atcacggggc gaggttctgc tggaacgcat tgccgctcag gcgaagcaga
     2641 gcggcttaag caaattgttt gtgctgacca cgcgcagtat tcactggttc caggaacgtg
     2701 gatttacccc agtggatatt gatttactac ccgagagcaa aaagcagttg tacaactacc
     2761 agcgtaaatc caaagtgttg atggcggatt tgggttaagc gatgaaaatc gccggatgcg
     2821 acatgcacaa cattcatagg tatgataaga cccacatgcg tcgcatcagg caattacgtt
     2881 tatccccgtg aactaaacaa cgccgccaga ccactgcgcc gctcggtgcg cgtggcaatt
     2941 gccgcactta aaatgcgctc atcggcatac agcgacagac ggcgacgcgc gcgggtcacc
     3001 gcggtgtaaa ccagctctcg cgttactacc ggcgtgcgtt gactcggtaa aatcaacgcc
     3061 gcatggtcga actccgatcc ctgcgattta tgtaccgtca tcgcccacgt cgtttcgtgc
     3121 tctggcaggc gactcggttg cacagactta atattgccgt ccggcatcgc aaaccagacg
     3181 cgcgtcccct gcccgcgatc cagcgcaata ccaatatcgc cattaaacaa cccaagcgcg
     3241 ctgtcattac gggcaatcat caccggtcgg ccttcgtacc aacgagagtg cggatgacga
     3301 tgaattttgc gcttctgttg cataaactgc tcaattcgct cattcagtcc agccacgcca
     3361 aacggccctt cccgcagggc gcacaaaagc tggtactcat tgaacgcctg aatgattaaa
     3421 tccggctcgg cacgcgcttg cagcagatcc agataacgtc cgtaacccgc aagagcttcc
     3481 tcaagcatcg caatataatc ttcgccgctc tgtaaaagtc gtttttcgat atcagtaaaa
     3541 tcctgctgaa aaacggtttt cactgccgtt ttatcaccac ggttaatcgc cgcagctaac
     3601 tgaccaatgc cagaatcgct gccgaaacga tagctttttt gcagcaggca gaggctgtcg
     3661 cgcaaagatg ccgcttctgt gccagttcct gctggaacgt ggcttcccgt caggcggctc
     3721 aactgcccgg cacgctcggc ggtaaagccc gcgttggcat aagcgcagat atcgcccagc
     3781 acagccccag cctcaaccga ggccagttga tcacgatcgc cgagaaagat cactcgcgca
     3841 tgatcgggca aggcgtcgat cagtctcgac atcataggaa gatcgatcat tgacgcttca
     3901 tctaccacca gcacatcaag atgcagcggg ttaccggcat gatgacgtaa acgctggcta
     3961 cccggctgcg cgcccagcaa tcggtgcaaa gtgctggcat cttccggaat gcgtttcttt
     4021 tgttcagcgg tcagcggtaa ctgtcgcaaa gccttgccga gagattcggt taagcgcgcg
     4081 gcagctttac ccgttggtgc agccagacgg atacggcagc gttctccgtc ggccatttga
     4141 attaaggctg ccagcaactt cgctacggtg gtcgttttac cggtgccagg accgccggaa
     4201 atcaccgaaa tccgccgcgt cagcgccact gccgccgcaa ctttttgcca gttaatttca
     4261 tcgcttactg gaaaaagttt gtccagggtt tgcgccagta gagcttcatc aacctcaatg
     4321 gcatgattca cttcgttgaa aaaacgtgcc actgtgcgct cgttacacca catgcgattc
     4381 aaataaagac gatcgccaca gaggatcatc ggcgttggtt catctccccg gctgaccgct
     4441 tgtgaagcca gcaagcattc ttcccaattt tgtagctcac cgatttcact gacacaggtc
     4501 gccaacagcg gatgcgacgc ctcgttattt tccagtcgtg aaagcggcaa acaaacgtgt
     4561 ccctctccgg catcatgact taatagtgcc gccgcgaggg tgacggcagg atgttcatct
     4621 cccgccacgg tcagggcaaa ctgcacgtcc agcgggcgta gctgtttgtg ctccacagct
     4681 tccagtaatt gcttttgcaa tttcattacg cctcctccag ggtcataccg gcaaacatct
     4741 catccatcag gtcaatcaac ccggcgttgg gtcgggtcgc gtagatccct tgttgcggat
     4801 gttctttatc aacgccacgc aggaacagat aaataacgcc gccaaagtgg cgctcatagt
     4861 cgtaatcagc aatacgatgg cgcagataac ggtgcagcgc cagggtataa agctgatatt
     4921 gcagatcata gcggtgtgcc tgcattgccg ctgccatagc ctgttgggtg taagccgaac
     4981 tgtcttcacc caaccagttg gatttatagt cgagcaggta ataacgccct tcgtggcgga
     5041 acaccagatc gataaagcct tttaacatgc cacgtacctg catgaactcc agcggcgggc
     5101 agccagcaga tagcgggtca aactggcgaa ttagtgcatc aagctggctg gcgatgagcg
     5161 gttcgctaat tggcaggtaa aactccatct ccacctgttt atcgcgatcg gaaagctgac
     5221 tcaggctaat acccgtttca ttgagaggtg ccaggaggac agccgtgatc cactcagtca
     5281 acaccggttc ccactgcggt tcaaagccac cgagctccag tttttcctgt acccagttcg
     5341 ggtcaaccgg ctgggtaaaa tcgaggtctt caaacaaact gtgcaagaat gtccccggtg
     5401 acgcaccgcg cgggaactga tgcggtgtta acgtcggtac ttcaacgacg ctgaccacgc
     5461 ctgcggcatc aacatccaac cgtggcatca gatcctgagc gataccgtga ccacgctgct
     5521 gcaaaccgga gtagctggtg acacgccagt tatcgccggg caatcgttgt agcgtcctcg
     5581 cattcagttc tgcagttaat gcatcattaa cctgccaggg ctggttatca ccaatttgtg
     5641 ccgtttgcca ggcaatatca tcatcgcaca acgcttcaat gcaggtacga agccctgcca
     5701 catcttgcgg ttctcctttt tgcagcaaac gcccgagcgc actttggtgg acgtcggtat
     5761 cacctttttt atcgccacga cggcgcacca gcggtgcaac gccgagactg caatgccaaa
     5821 ccgaacgcgt cagcgccacg taaagtaaac gcagatcttc cgccagacgt tcggcctccg
     5881 cgaggtcgac gctttctggc gcagcattaa gatccagaac ggcctcaaac gagtggcgat
     5941 cgtgataaaa cgcctgatcc tggacgcgga aattggtaat aaatggcaac cagaccagcg
     6001 gatattccag cccttttgat ttgtgtatcg tgacaatctg caccagatgt ttatcacttt
     6061 cgaggcgcat ttgttggctg gaggcattac tgtctggctc gaggatatgt tgcgataacc
     6121 agcgtaccag cgcatgttca ctttccagct gcgttccggc ttcttgtagc agttcgctga
     6181 tatgcaagat atcggtaaga cgccgctcac cgcctgccgt tgccagcaag ttttctgcaa
     6241 tgttacgcgc cgacatcagc gcccgcagca tcggcataac gccacgtttg cgccagattt
     6301 gccgataacc atcgaactct tcgactaccg catcccacgc atgttcgtca ttattcagcg
     6361 tttcaatatc cagcgcattc agccccatca ttgacgttgc cagcgcactg cgcagggtgt
     6421 tctcacgttc gggcgtcatc accgcctgca acagccaaag catctcctgt gcttccagag
     6481 tttcaaaaac actgtcgcgg ttcgaaaggt aaacggaagg gatttccagc agcgttaagg
     6541 catcgcgcac ctgagcggcc tcctggcggc tgcgcaccag cacactgatg tccgaagcac
     6601 gcaccggacg cgcatcgtcg ccgttcatca gcaacgcttc gccccgctgt ccggcttgta
     6661 gccagtcgcg gatttgcgca gcacatacct gcgccatggt actttgatag tcgccaacgc
     6721 cgcagctttc gccttccatc agccacattt tcatcgcagg ctgcatttca cctttaaaca
     6781 caaagcgtaa cgcctgattt ttcccggcag atttcacagg aataaacggt atttcacgaa
     6841 acatgaacgc gtcatcagtc tggctgaaaa gtttgttaac gctgttcacc attcccggtg
     6901 cggaacgcca gttggtatct aaggtgtagt gagcatgaac ttcgctacgc gccttcatat
     6961 aagtgaagat atccgcaccc cggaatgcat atatggcctg tttcgggtcg ccaatcagca
     7021 acaatgcggt ttccggctga tggtgccaga tacggcgaaa aattcggtac tgctgggggt
     7081 cggtatcctg aaattcatcg atcattgcca ccgggaatcg cgtacggatc gccgctgcca
     7141 atacctcgcc gctttcacta cgcagcgcgg aatcgagccg acttaacatg tcatcaaaac
     7201 ccaattcgcc acggcggcgt ttttcacgcg ctactgtttc gcggatctca gccaatgcgc
     7261 gggtgatcac cagatcgcgg atcgacaatg gttctgcaag cagttgatcg atcgcctcaa
     7321 acagtggatg tcgcggggtt tcccccccgg ctttcgtgcg atcttctaag aaacgctgag
     7381 agaatttttc cagcgactcc ggcaactgat agctattggt ctcttcttct gcccaggcgc
     7441 tgatcttctc gatccattta gcctgattgc tacggttaaa cttgcgtcga tcaataccag
     7501 aagattcgat cagcgcatcc agttcaccca ctgcgtcgcg ccactgctgt tttacggcat
     7561 caatacgcgc cacaatttgc gcgtggcggg aagccagcgt ttcatcatcg gggggcggtg
     7621 ctttgataac tggcgcttcg ccttgcagat aacgattaat atcgcgcagc aacgcctgcg
     7681 gccctttcca ggtttcaaag acgacctggg caatttcacg cggcagcggg tagcagtggc
     7741 gacgccagaa atcggcgcag gcctggtagc gtagcagaga ctcatcttca atcagctgct
     7801 gctcaaacag catgccggat tcaaaggcat tcaggttgag catgcgctgg caaaagccgt
     7861 ggatagtaaa gactgccgct tcatccatct gccgctcggc taacaacaac cactgcgcgg
     7921 cttgcgcttt atcgtcgatc tcttccagca ggcgtttgta cagtggattg tcggtggttt
     7981 cacgcagaca ggcgatgcgc aactcgtgga tattgctacg gatacgacca cgcaattctg
     8041 ccgtggcagc ctcggtaaag gtcaccacca gcagttcttc aacggtcagc gggcggggaa
     8101 aggcggccga accgcctagt ccaagtaaca ggcgcaaata gagcgccgca atcgtaaagg
     8161 ttttgcctgt gccggcagag gcttcaatca ggcgctcgcc ctgtaagggc aagcgcaaag
     8221 gatctagtgt ctcggcgaca tcactcattc ttttcactca tcaggggcat tgtttgctgc
     8281 aacgcgctga cgttctccca cactttccag ccttcaggat gcacatattc ggctttccca
     8341 ttctggctgc cggaaatctg cgacagaata gccatacctt gcggctcgac caccgcctga
     8401 tggaagaaat cagcaatttt ttgcggcgtc agcagtttta tctgggccac gattttatca
     8461 cgcgaatcga agcgcatatt gccgcgatcg aaatctttac ttaacttcga tgcttcttcg
     8521 ccgagcgttt gcggtgcctg cagcatctgg gtaattaccg cctgctggat ttgcgcaaac
     8581 tcttctggct tcatcgcccg caattttgcc tctgcggttg ggaaaaacgc cttgtaacgc
     8641 tcccacaaga atgaaggctg tttatcattg ctttgcaaaa ggaagcccat gccccactga
     8701 cgccccacgc tcattggaaa cgcaaacacg gcatagccca actgttcttc ggtacgcaac
     8761 tgattgtaga accacggctg tacgatctgc cccaacagag aactatacgc tgagctggtg
     8821 tattcatcgt agccagtcgg tacaaatacc gctgccagtg cggagtcggc gctgttaccg
     8881 gctttttcaa agatgacgga ttgtttttta tcgaccacga catctttgtt acgacaccac
     8941 tctgaaccat cagcgcccaa ctgtttttgc acatggcgtg ccagcgttat tgcctgggct
     9001 tcagtcatgt tgccgataac cataaactct ggtctagccc ctgattttaa ggcgtcgcga
     9061 taggccagca cctctttcaa cgtaatggag ggcaaaattt tgcgccgttc atctcgcgag
     9121 aagtacggca cttgcgagag catctgcgcg ggcataatcg cctgctcgaa cgctttgccc
     9181 ttttctgcgg aatccatcat ctggttatac caggacttcg cctgctcaag ctgatcttcc
     9241 gtagcggtat agctaaagta gccctccagc agagcctgga acagctgcgg cagacgctgg
     9301 gtgtaaccat tagcattaac cataaggccg ttgttagcat tggtggaaaa acttatgcca
     9361 ccaaccgacg cctggttgct taactgatca agcgccagcc ctgcgagata atcattgagt
     9421 gcaaacatca cctgattgcg ggcgctgtcc atggctttcg gattacgcaa aatcaggctg
     9481 acatcagctt tgggctcgct ggcaaaataa cggcttggcg catacaccac gcgcagattc
     9541 gactcatcaa caatcaactc tggatggtcg tatttcttct ctgacttaat cagcgagaaa
     9601 tcgtcaggaa tataggggtt aagctccggt aaggagagcg caatattggc ggcttttttc
     9661 tgccagtcag cgaaagtttg tgcgctaatt ttatcgacct gatacggcgc atcgacaaag
     9721 taagccgttt tgttgtgcgg ctctttcggg ctgatatacc agatacgcgc attctgtggc
     9781 gtcatcatcg ccagacgttc ttttaccgct ttagcgtcgt accgatcggc aatattgact
     9841 gcatccagtg tatgctcaac aggaacgcga atcatggtat ctgccagcca ttcgacgtaa
     9901 tccatatcac gggtgattga cggataacgg aagtcgatat ccagcacatt cgccagttca
     9961 tcgaagtatt gtttatcgat ccctttttca cgtaacagat tgaggtagct aaaaatggcc
    10021 gccacaacct gatcgcgatt cgccagacct ttatcggtta aagacgcaga gatcgctaat
    10081 acgccgctgt tgccgttgac gataggatcg gaattggcgc taatgccctc aactaatccc
    10141 tgcttttgca gccagtcaga aagtgtacca gggctgcgat taccaatcag ataggtaatc
    10201 aattcatccg ttttactacg gaacttcgct gaattgttat cgatgcgaaa ctcaacgcgc
    10261 agaactttac gcggcagcgc cgggacgtaa tgaatgataa tgcccttttg cgcgtcggtg
    10321 actaccggca cggtgatttc cggttttttg ctctctttgt tcggcacgcg accaaaggtg
    10381 tccgccgcca ttttcgccaa ctccggcaac ggtttattgc tgtaaataac cgccttcatc
    10441 agattggcgg aatagtactt ctcgtggaaa tctttcagcg cctgctgtac cggattacct
    10501 ggtttgtcgc ttaaagtttc gaggttacca ccagaaaact ttgaaccggg gtgtgccggg
    10561 ttaatggttt ctgcgctgac ctgtgccatg cgcatcccgt cacgcgtacg cgccatggtt
    10621 aattcggcat tcactgcatt acgctcacgt tcggcgtatt tcttgtcgag caagggttct
    10681 gcaatagcat cggccaggcg gtctaccgca ccgggtaagg catcgttctc aacttccaga
    10741 tagaaagccg tgcgatacgg tgccgtgctg gcattgtgac tgccgccgtg cattttgaga
    10801 tattcggcca gactgtcagc ctgcggatac tttttcgacc ccatcagact catatgttca
    10861 aggtaatgtg ccagcccctg gtacgcctca ggatcttcca gcgacccaac gggcaccacc
    10921 agcgccgaga gcgatttaac tgcctgcgga tcagaaacca gtaagaccac cataccgtta
    10981 tccagacgta tagcctgata ctggcggtta tctttatcac ttttacggat ggtttcctga
    11041 atcggctgcc atcccgtttc tgcctgactt aagggtgccc agagggcaac aaacaacaat
    11101 aatgctttga accagatgct gcggggcatt cacggacctc ataatcaact ttattttctg
    11161 gccagattca acacgttaac gcaaatcatc tgccagaatt taatcttgtg ctgaacgagt
    11221 cagcctatgt ctatataacc atcagtccgt gactggtgcg catcataaag taagcggata
    11281 gattgcgcaa tttttataca acactcatga ctgattaaag cgaaacagcg gtaacaggaa
    11341 acgttgcgac tgttcaacga tagcctccat tgtctctggt gttaattgcc gccagagccg
    11401 ttgataccag atatcatcac cttcgccacg caccatcatg ttgccttcgt aagcctgaag
    11461 gaatttcgta cgggcttttt gcaacgtgga atcgtcatcc agcatggcat cgttttgcgc
    11521 gtcataacag gtttttagcc acgcgccgcc actttcaggt aacaccagca atggcgcgga
    11581 cattccttca cgatacccct caatcagttg tgagaggtaa tgcaaagcct gttcggctgc
    11641 aagcggcgga aaacgccact cgccgtcttt gcgtagaaaa aggcgacttt caccattacc
    11701 accgctggca cagtagacaa ggtgttccag ccaaagttgc attccctgcg ccacacttaa
    11761 taaagaggga cgccagcgca acaggccatc cggctgcacc tgcggcaacc agcccgttat
    11821 ttgcacaccg ttgcaggcga gatcaatctc catactctgc cccggctggc gacaggcaat
    11881 gactctgtcg gcaagctgct gcatctcctg acactgcgtt tcccagaaaa tttcaccaaa
    11941 agcgccatac ggtaaatccc ctgccgcccg gaagcgacgg aacaagcgtt cggcatcatc
    12001 ctgctcaacc agtgcattca ataactgctg attgatttga taacggctaa gtccttccag
    12061 aataaatggc tcggtgtcgg ggatttcgct gtcttcggta cggaagttca cctgcaaacg
    12121 catctggaaa aatgcccgca ccggatgtgc ccagaatcgt tgtagcgttt ccagcggcac
    12181 ggtttccggt aaggtaaatg gcagcggctg aacaaattca gaatgtgctt taccagcctg
    12241 gctggccgca ggtagccatt cacgagcata actttgtcgt tcgcctggct ggtagttttg
    12301 tggatcaaac ggcattcggg tatggaggca agtaagatgc gcttttaccc ttgcctcgct
    12361 ttcatcacag ttgagcgctt catcgcccgg tagataatgg ctttgcccga tgtagtcgat
    12421 cagttcctgc accagtaccg acgggaaacg ttcactgtta tcctgaatgg aacgcccgat
    12481 atagctgata tagagttttt gctgcgcgga aattaacgct tccaggaaca gatagcggtc
    12541 gtcatcgcga cggctacggt cgccacgctt cggtttctgg ctcatcagat caaagcccaa
    12601 tggcgcaagc tgacgtggat aaacgccgtc gttcattccc agcaggcaaa ccactttgaa
    12661 cggaattgaa cgcattggca tcagagtaca aatgttaacc ggtccggcga gaaaacgctg
    12721 gctgatacgt tcttgatcca ggcgctgtgc cagttcatca cgcaatagtg acagcggcac
    12781 cgcgtcgcca tactgcgcac ctaaaccttc ggcgataatc gcctgccatt gttgttcgat
    12841 cagcgtcatc gccgcttcgg tttccgcatc cggcaggaag aaggcgttga gcatatcgcg
    12901 acaaaccggc aaccactctt ccagcggacg ctcctgcgcc agcccgcgac gccagatatt
    12961 tagctgcatt agcagtgaag ccagatgccc caccagttct gcaattaagc cgctcgattc
    13021 atcataaggt agaaccgatt gccactcgcc ctgcgcgctc tccatcgcgt agcccagcaa
    13081 catgcgcgtc aggccaaacc gccaggtgtg ttgaccggta gcgggaagtt ccagctcgcg
    13141 aacgttgtcg tcatctatcc cccaacgaat gccggactcg ttgacccact ggcgtaaata
    13201 acgcagccct tcttcggtga tgtcaaaccg cgccgccagc accggcacat ccagtaatgc
    13261 cagcacgtct tccgacacaa agcggctgtc tggcaacgat aacaggctga taaacgcttc
    13321 aagtacagga tgcgactgcc gcgcccgacg gtcggaaatg gcgtaaggca ggtaacgatc
    13381 cgcaggtgcg ctaccaaaca cagcctgaat aaacggactg tagctgtcga tatcagccac
    13441 catcacgatg atgtcgcgcg gagtaagtgt cgggtcttcc tccagcatcg ccagcaggcg
    13501 atcgtgtaaa acttcaactt cacgctgcgg gctatggcaa acgtggaagg tgatactgct
    13561 atccagtgga tcaagcgggc gtttgttatc gctacgggaa aactcttcga tgttcacacc
    13621 agcaacggcg cggttttcca gttccagaat gtcagactga atattatgca gcaggttatc
    13681 tggcgtcaca tcgacaaaag cgtccagctc ctggctgctc tccaggtcag aaaggagata
    13741 aatgtagtcg cgcccaagct tgccccatga agccagcagc gggttgccga catcctgttc
    13801 accatcgctg ttaaagagct gcccggcatt ttcgctgtcg cgaaatagcg gtaattcgcg
    13861 atcttcaaaa ctgtgtcggc gctggcgagt cagtagtttc gccagataag cgggatcttt
    13921 aatgtcgccc cagtaataac ggcaggggtt ggtaaacagg agatggattt caatatgttt
    13981 acccagcgcc tgtagcgcct ggagataaac aggcggtaac gcggaaatac cgcatataaa
    14041 gacgcgcgaa ggtaaccccg gcgggcaggt cgtcgcggac tccagcgttt cgataaagcg
    14101 ctgatagaga ttggcgcggt gccagcgcgg ttgcccaagt tcgtcggtat attccaccaa
    14161 cgccttccac aacggcgctt gccaggcctg tgcttctccc aacccttcta ccagatgtcc
    14221 tgtttcccac tgtgccagcc agtccggacg atagaccaga tactggtcaa acaggtccgc
    14281 cgcttttgaa gaaagctgga acagttttcg cttgtcacta tcgtcagtca gataatgccg
    14341 caacagggta aagtcttcgc gctccaacag ttgcggcagc agtgtcatca gtttccagct
    14401 catgctctgt ttgttaaagg cgctctcttt ggggatctcc ggtaacaccc ggacgaacat
    14461 atcccagata aagctcgctg gcagcggaaa atcaatgttt gccgcaatac caaacttttg
    14521 cgacagggtc atttgcagcc actgtgccat accggtactt tgcaccagaa tcatctctgg
    14581 ttcgaaagga tcgtccagcc gttcgcgttc gacaataaac tccatcaacg cttccagcac
    14641 gtccagacga ttggaatggt agacccttaa catagcggct cctgactact gacgattcgg
    14701 gcaatgcagg cgggtcatct cgccttctct gcccccgggt gaaactagcg taacgctgat
    14761 gctgacacat cccgcctgcg atgtctgcat tcggttgacc tgccagttag caggtggcga
    14821 aatcgcgcgc agttgcgttt gctgccagcc atgccgccag agctgttggt actggtttct
    14881 gctggcaaga ctgttcatta atgttcgctg gaaacccgat aacgcagtga caatcatcac
    14941 catcaacacc atcgccaaca ttacctccgg cagactaaag ccttgttgat tcctcaggga
    15001 agctgacata acgccctctc tttcagcgga caaaaatcgc tccagccgcg tggcgaaaaa
    15061 acaatgtttc catcgatgac ttcgcctgtt cgccacaacg aaacgccttc ataaccagca
    15121 atcaataagg cttcattatc tgccagtaaa cgcagacaaa cccgggcacc ggtggcagca
    15181 tacagtaagc actgaactgc gggcagcgtc tgccaggagt gcatttttcc ccactccagc
    15241 gccgactgaa cgatggcctg gcggcgcaat gactgacttt ccatgctcac gcgagaagca
    15301 aaactgcgat cctgctgact cattccttgc aatagcaagc tacccaaaat cagcagcatc
    15361 aggaccagag ccagtgacga aacacccttt tcgcggttca caggttggat cctgtcacgc
    15421 tatagctggc atccaccacg gtttgcggat cagccttgct ggcagcgtgc atattaaccg
    15481 tcaacaccgg cgagaagccg ctgacatcct gacgtacgac ctgaaaagtg tcgataatga
    15541 tggcatccgg attagtgact ttatcccagc ccttaccttc acaggatgtc gcaccgcgta
    15601 gcgtttccag cacatgctcc ttcagacgaa atccaatctg gtcggactct tttaccggtt
    15661 cgcgatccca gataccatta ctgttcgcat cccactgcac aatgacacag tcaccctgtc
    15721 cgacaatttc cagcccttcg cccgtacagc tgccatggca ataacccgcc ctctggagat
    15781 gcttcgcgac ggtaaatacc cgcagccaga tttcatcttc cagcgccagc ttacgggtgt
    15841 tcgttaaact ttcacgctgt aacgcaggca gaaagcgtgc agcccccagc aacaatacgc
    15901 tactgatcgc catagcaatc aacacttcca gcagagaaaa accttgctct tttacaggca
    15961 tccttctgtt tctccttgct gacaaagccg gagtcttccc cacggcgaaa ccactagcca
    16021 ccactcgccc gttgagtttt tgaagcgaat atgcccggcc catgcggtat tgcgcaggcc
    16081 aaagaaagca agcgaaggtg tcaggtcgct catttcgact tcgggccagc gcggcacaaa
    16141 gaccaatggt gaactgccat gacaggtatt cgccccagca acggaactca caaggcacca
    16201 taacgtcccc tccctgataa gactgatact gtggtcgcgg ttatgccagt tggcatcttc
    16261 acgtaaatag agcaaatagt cccgcgcctg gctggcggtt tgccaaagcc gttgcgactg
    16321 ctgccagtat tgccagccat agagcccact tgcgcttagc atgaccagaa tcagcatcgc
    16381 gaccagcgtt tcaatcagcg tataaccacg ttgttttttc atgccggcag tatggagcga
    16441 ggagaaaaaa agacgagggc cagtttctat ttcttcggcg catcttccgg actatttacg
    16501 tcgttgcagg acgttgcaaa atttcgggaa ggtgtctcga agaatttaac ggagggcaaa
    16561 aaaaaccgac gcacactggc gtcggctctg gcaggatgtt tcgtaattag atagccaccg
    16621 gcgctttaat gcccggatgt ggatcgtatc cttcaatctc aaagtcttcg aaacggtagt
    16681 cgaagatgga ttcgggttta cgtttgataa tcaacttcgg cagcggacgc ggttcgcggc
    16741 ttaattgcag atgagtttga tccatatggt tgctgtacag atgcgtgtcg ccaccggtcc
    16801 agacaaaatc acccacttcc agatcgcact gctgcgccat catatgcacc agtaacgcgt
    16861 agctggcaat gttgaacggc aggccgagga agacgtcaca ggagcgctga taaagctggc
    16921 aagagagttt gccgtctgcc acatagaact ggaagaatgc atggcacggt gccagcgcca
    16981 ttttatccag ttcgcctacg ttccacgctg aaacaataat gcgacgggaa tccgggtcgt
    17041 ttttcagctg gttcagtacc gtagtgatct ggtcaatatg acgaccatcc ggcgttggcc
    17101 aggcgcgcca ctgtttacca tacactggcc cgaggtcgcc gttttcatcg gcccattcgt
    17161 cccagatggt gacattgttt tcgtgtagat aagcaatgtt agtgtcgccc tgcaggaacc
    17221 acagcagttc gtggatgatg gaacgcaggt ggcaacgttt agttgtcacc agcgggaatc
    17281 catcctgcag gttaaaacgc atctgatgac caaaaatgga aagcgttccg gttccggtac
    17341 ggtcgttttt ctgtgtgcct tcgtcgagca ctttttgcat cagttctaaa tactgtttca
    17401 tggttcctca ggaaacgtgt tgctgtgggc tgcgacgata tgcccagacc atcatgatca
    17461 cacccgcgac aatcatcggg atggaaagaa tttgccccat gctgatgtac tgcacccagg
    17521 caccagtaaa ctgcgcgtcg ggctggcgga aaaactcaac aatgatgcga aacgcgccgt
    17581 aaccaatcag gaacaagcct gagacagctc ccattgggcg cggtttacga atatacaggt
    17641 tgaggataat aaacagcacc acaccttcca gcagcagctc gtaaagctgt gatgggtggc
    17701 gcggcagcac accgtaagtg tcgaaaatgg attgccactg cgggttggtt tgcagcagca
    17761 aaatatcttc tgtacgggag ccagggaaca gcatggcaaa cgggaagttc gggtcaacgc
    17821 ggccccacaa ttcaccgtta ataaagttgc ccagacgccc ggcaccaaga ccaaacggaa
    17881 tgagtggagc aataaaatca gagacctgga agaaggaacg tttagtacgg cgggcgaaga
    17941 taatcatcac cacgataacg ccaatcaggc cgccgtggaa ggacatgccg ccgtcccaga
    18001 cacggaaaag atacagcgga tcggccataa actgcgggaa attgtagaac agaacataac
    18061 caatacgtcc accgaggaag acgccgagga agcccgcata gagtaagttt tcaacttcat
    18121 ttttggtcca gccactgccc ggacgattcg cccgtcgtgt tgccagccac attgcaaaaa
    18181 tgaaacccac cagatacatc agtccgtacc agtgaagcgc cacgggtcct attgagaaaa
    18241 tgaccggatc aaactccgga aaatgcagat agctactggt catctgtcac cacaagttct
    18301 tgttatttcg ctgaaagaga acagcgattg aaatgcgcgc cgcaggtttc aggcgctcca
    18361 aaggtgcgaa taatagcaca aggggacctg gctggttgcc ggataccgtt aaaagatatg
    18421 tatatgatcc gcgctataac cccccacgaa tcagcccgcc catgccgcga cgctccataa
    18481 aggctgcaac ctgatggcga acttcggtcg ccagttgcgc ttccagacta cgctgcgcaa
    18541 gattttctgc ttcggcaaaa tcaatgcgcc gcagcaggta ttttacgcgc gctacagaac
    18601 gtccgttcat agacagatgg cgatacccaa gcccaatgag gattgccacg cacatcggat
    18661 cgcccgccat ttcaccgcac aaacggagat cgattccatg tatttccgct tcccgggcga
    18721 tcatcgccag agctcgtaac attgcaggat gaagactgtc ataaatgttc gccacccggg
    18781 tattgttgcg atcaacggcc aggatgtatt gcgtcagatc gttggtgcca acagagatga
    18841 aatcgacccg ctttgccaga tgcggcagca taaacaccat tgacggcact tccagcatga
    18901 tgccgatacg tggtttggga atttcgtaac cgatcatctc ctcgacttca cgtccggcac
    18961 gttcaatcag gcggcgtgct tcgtcaactt catcgaggct tgtgaccatc ggcaacagaa
    19021 tattcaggtt gcccgtagcg gcattagcac gcagcatcgc ccgcacctgg atcaagaaga
    19081 tctccggctg atcgagcgta atgcgaatcc cacgccaacc caggcatgga ttctcttcgc
    19141 tgatcggcat gtaaggcagc tgcttatctg ctccgacatc cagcgtacgc aaggtgacgg
    19201 gtttatcatt aaacatttgc agcatcccct gatactgcgc cacctgttct tcttccgacg
    19261 gaaaaccact ttgcagcatg aatgggattt cagtgcgata aagtcctatg ccatcaatac
    19321 ggctgcccag tttttcttca tgttccgggc ttaaaccggc attgagcatg actttaatgc
    19381 gttcgccgct ttttaactgg gcgggtaaat tgacgtcatc ttccgccaga cggctaagct
    19441 cgatctcttc actaattagc cgctgatatt cttgcagcag taccggctcc ggatcgacca
    19501 gcaattcacc gcgataacca tcgacgatca gcgtccgacg atgcagtacc gaaggctgaa
    19561 tatccgcgcc catcacggta gggatcccca gcgcacgtac catgatcgca gcatgggagt
    19621 tggcagcgcc atcgcgcacg acaacaccga ctaagcgatc ctggggcagc tcagcaagcg
    19681 ttgtcgctga cagttcatct gccaccagaa tgaaacgttc cggccaggcg ttcggccctt
    19741 gattagcgtc atcaagatga aacagcaatc gctgaccaag tgcacgtaaa tcgccagccc
    19801 gctctttgag atagttatcg cttagcgcgg caaactgttc ggcaaatttt tcaatgaccg
    19861 tttttaccgc ccactctgcc accgagcctt tatcaacctc ggcaaacaat tcgcgacgca
    19921 gccgggtatc cgaaagcagg tgtgagtaaa gatcgaaaat agccgccgtt tctttttgtg
    19981 caccggcagc aaagcgtttg ctatagcggc gaaactcgtt agccgcctct tccaacgccc
    20041 cggtcagtcg ttcgcgttcc agagccggat ccagcgttga tgcctgatac acctgttcca
    20101 ttaaaggtaa cgtggcatcc tgccagcctt cggcaatcgc cacaccaggt gctgccggta
    20161 atgcacggat tcgcgtctgg cgatattgcc caaacaaggc agtcaactgc gactgagaaa
    20221 gaatagctgc catctgggtg gcaagcgtca ccaggaagga ttcttcactt tcgtcatact
    20281 ggcgcaactc tcgttgctgt accaccagta caccaagcaa ctggcgacgt tgaataattg
    20341 gtacgcctaa aaacgcgcgg aaacgttctt cttttacgga ggggatgtat ttgaagctgg
    20401 ggtgcttttg cgcatctgca aggtttatcg gttccgccag cctgccaacc aggccgacga
    20461 tcccttcatc aaacgcgagc gttacagtgc gaccgcgtgg ttttttcagt ccccgggtcg
    20521 ccatcaggta gtaacaacgt cgatcatgat cggccaggta gaccgaacag acctcggtat
    20581 ccatcgcaag acagatgtcg gtaaccagaa tatttaacgc ctcattcagg cgtggtgcgc
    20641 tggctaccct ttcgactatt tcgcgcaggc gagtgagcat aattggcgtg acttaacctc
    20701 ttttacgtcg ataagcagat gcgttttgtg gttttggcgt attttcctgc agtgacatca
    20761 ccacactcgc gaactctttc attaccctac ggtagacatc acgtttaaat gacaccacct
    20821 gtctgactgg ataccagtaa cttacccatc gccagccgtc aaactctggt gtactgctgg
    20881 tttgcatatt gatttctgca tcgccgctca ccagctgcaa gagaaaccat ttttgttttt
    20941 ggccgataca aaccggcttc gtgtcccaac gcaccaaacg tttcggtaat ttgtagcgca
    21001 accagttacg cgttgaagca aggattcgaa cgtctttgcg gcttaatcct acttcttcaa
    21061 acaattcacg gtacatcgcc tgctctgcgg attctccggg gttgattccg ccttgcggaa
    21121 attgccagga gtgctgacca aatcgccggg cccacattac ctgcccctgg cgattacaaa
    21181 tcacgatacc tacgtttggg cggtagccat cgtcatcaat caccggacta cctcaaaata
    21241 aagctttata tacgaatgat tgtttcatac tccaggaaga cggtaaacca ctctctgcag
    21301 ggcattacac actaataaca attgaataac tcacagttat gtgcagagtt ataaacagag
    21361 gaaggggtgg atagccgttt ttattcacct tttctgtgga tagagttgtg aagaactacg
    21421 gaattactac gggaaaaccc ggagcattct gaataagaca gatatagaga ttttaaaata
    21481 ttcattatca gtcaatgtat tacatgtgat ttgagtaata aaaaagagta attcgtgacc
    21541 caggtcacac ctctcattta cgggttggcg aaagatcaac caacgccgta tttatccaca
    21601 gaatgtgcca ctaagttaag cactgaacca ctaaaaactg gagtttcgtc gcacgtcaag
    21661 gctgtaaatg gaaacagtag tgaaggtttt tcacagttat cccagctttc tgtggataac
    21721 atggtgtaag atcctgttta ttttcagtga ccagatttgg aaaacccgtt gcagtgttgc
    21781 gcaactcgat taccggcaac ctaaaaagca atataaatca gagaattgaa caacgcatgt
    21841 ggaaaaagtt acactgcgaa tattcggcac ataattgctg tttgtttttt aaccaaggta
    21901 tcatgacatg tcccaacctc gcccactgct ctctcctccc gaaactgaag aacaattgtt
    21961 agcgcaagca caacaacttt ctggttatac attgggagaa ctggcggcac ttgccgggct
    22021 ggttacgccg gagaatttaa aacgcgataa gggctggatt ggcgtgttac tggagatctg
    22081 gctaggtgcc agcgcaggga gtaaacctga gcaagatttt gctgctctgg gcgtggaact
    22141 taaaactatc cctgtggata gtcttggtcg tccgctggaa acaacattcg tttgtgttgc
    22201 cccgttaacg ggcaatagcg gggtgacatg ggaaaccagc cacgtgcgcc acaagctcaa
    22261 acgcgtgctg tggataccgg ttgaaggcga gcgcagcatc ccgctggcaa agcgtcgcgt
    22321 aggatcaccg ttgctgtgga gcccgaatga agaggaagac cggcagctac gcgaagactg
    22381 ggaagaatta atggatatga ttgttctcgg tcagattgag cggatcaccg ctcgtcacgg
    22441 ggaatattta cagatacgac cgaaagcagc gaatgcgaaa gcgctgaccg aagctattgg
    22501 tgcccggggc gaacggattc tgacgctgcc gcgcggattt tatttgaaga agaatttcac
    22561 cagtgcgcta ctggcccgtc attttctgat ccagtagcca tcgctttgac ctgccgcttt
    22621 ccgggcatat aattaccgct tcattttttt ggcagggctt tttagatgtt atttgcatgg
    22681 ataaccgatc ctaacgcctg gcttgcgctc ggtacgctga cgctgctgga gatcgttctt
    22741 gggatcgaca atattatttt cctttctctg gtggtggcaa agctcccaac agcacaacgt
    22801 gctcatgcgc gccgtctggg gttggcggga gccatggtca tgcgtctggc gctgctggca
    22861 tccatcgcct gggttacgcg cctgacgaat ccgcttttta caatattcag tcaggaaatt
    22921 tccgcccgtg atttgattct gcttctgggt ggcttgttcc ttatctggaa agccagcaag
    22981 gaaatccacg aatccatcga aggtgaagaa gaagggctga aaacacgcgt ttcatcattc
    23041 ctcggcgcta tcgtgcagat tatgctgctg gatattatct tcagcctcga ctcggtgatt
    23101 accgctgtgg gtctgtcaga tcacctgttt attatgatgg ctgccgtggt gattgccgta
    23161 ggcgtgatga tgttcgccgc gcgctcgatt ggtgattttg tcgagcgcca tccttcggta
    23221 aaaatgctgg cgctctcttt cctgattctg gtgggcttta ccctgattct ggaaagtttc
    23281 gacatccacg taccgaaagg ttacatctac ttcgcgatgt tcttctctat tgcggttgaa
    23341 agcctaaacc tgattcgcaa caaaaagaat ccgctctgat actccgtacg ctctcctgcg
    23401 ggagggcgta ctcactcctg ttaccctcac agtttaagat tttcctgctt tcaagatata
    23461 taacgtcggt ttataaacag actattatca taggtgggcc aaatgaggat aaccggatga
    23521 aaaaatgggc agtaataatt tctgcagtcg gactggcgtt tgctgtttcc gggtgttcca
    23581 gtgattacgt catggcgacc aaagatggcc gtatgatttt gaccgatgga aaacctgaaa
    23641 ttgatgatga taccgggctg gtgagttatc acgatcagca aggtaacgcg atgcaaatta
    23701 accgtgatga tgtttcgcaa attattgaac gttaacaaat aaggtcagca tccggctggc
    23761 cttaagattt ttctctttcc cttttccctt ccctctgcca tttttatatt ccttatgtcg
    23821 tgattataaa aaggaaacgg ctatgcaata tcaccgtata ccccacagtt cgctggaagt
    23881 cagcacgctg gggcttggca cgatgacgtt tggtgaacag aacagcgaag ccgacgccca
    23941 cgcacaactc gactatgccg tcgctcaggg cattaacctt atcgacgttg ccgaaatgta
    24001 cccagtacct ccgcgccccg aaactcaagg gctaaccgaa acctacgtcg gcaactggct
    24061 ggcgaaacat ggcagccgcg aaaagttaat tatcgcctcc aaagtgagcg gaccgtcgcg
    24121 caataatgac aaaggcatcc gcccggatca ggcgctggat cggaagaata tccgtgaagc
    24181 gctgcatgac agcctcaagc gtctgcagac tgattacctc gatctctatc aggtgcactg
    24241 gccgcagcgc ccaaccaact gcttcggcaa actcggttat agctggacag attctgcgcc
    24301 tgcggtttcg ctgctggata cgctggacgc actggcagag taccaacgcg cgggaaaaat
    24361 tcgttatata ggcgtgtcga acgaaactgc atttggcgta atgcgctacc tgcatctggc
    24421 ggacaaacac gatctgccgc gtattgtcac cattcagaac ccctacagcc tgttaaaccg
    24481 cagttttgaa gtaggtctgg cagaagtcag ccagtatgaa ggggtcgaac tgctggccta
    24541 ttcgtgcctg ggtttcggca cgctgaccgg gaaatatctc aatggtgcaa aacccgctgg
    24601 cgcacgtaat acgctcttta gtcgcttcac ccgctatagc ggtgagcaaa cgcaaaaagc
    24661 cgtcgcggcg tatgttgata tcgccagacg tcatagcctg gaccctgctc agatggcgct
    24721 cgcatttgta cgccgtcaac cgtttgttgc cagcactctg ctgggcgcaa ccacgatgga
    24781 gcagctgaaa actaacgtcg aaagtttgca tctggagttg agcgaagacg tattagctga
    24841 aattgaagcg gtgcatcagg tttatacgta tcctgcacca taagattaac gccggttaaa
    24901 accggcgtta aatattaatg acggcgctgc cagatccaca gcgccgttat tgccagcgca
    24961 aacagcgcac cgaagccaat gccaatgggc acgaccggga tgcctaccat taccgccagc
    25021 gagtaaatgc ccagcatcaa caacatggcg ctgttttcgc caaggttttg tactgcaatc
    25081 gcattccccg ccccgacgct ttttttaccc cgctcctgta gcaacgcatt gagcggaacg
    25141 acaaaaaaac cccccagcac gccaatcagc atcaacaaag cataggctgg cagcagctcg
    25201 tgttgcaggg agaaaatcag taccaccacg ccaatcaaaa tcccggctgg catacagcgt
    25261 gacacggttt ccagcgtaac taacttcgct gccgcacctg caccaaccac gataccaatc
    25321 gctaccatcg cgttgagata ggtcggcgta gcgttatcgg taatacccag cgccaccggt
    25381 acccacagca ccaacaggaa acgcagcgtg acacccgctc cccagaataa actggtgccc
    25441 accagcgaaa aacgcgtttc accattgcgc catagcgagg tgcaggcatt caggaaactg
    25501 cgggtcatgc tgatgagatt ccaggactgc cccggacgcg ccgccgccag tttgggaatg
    25561 tagatattgg caatgaccgc gccaccgtag gccagtgcgc atgcggccag ggcgatcagg
    25621 acatgccagt cagccagaac accaccagct acggaaccga gcaaaatcgc cgctatggta
    25681 gacgcctcca ttaaaccgtt agcttttact aacttactac ccgtggttaa ttcgccgaga
    25741 ataccgtatt tcgccggtga ataggctgca gcaccaacac ccaccaacgt atagccgaga
    25801 aacggattga taccaaagca gatactggct gcgcccagta gcttcaggcc gttggcaaac
    25861 atcatcaccc ggcctttggc gaagctatcc gccacctgcc cgacaaacgg cgcaaaaaga
    25921 atgtaagcac ctacaaacac catttgcagg atgggctggc tccactccgg atagaactgc
    25981 gctttcagta acgccagagt agcaaacagt agcgcattat cgccaaacgc agagagaaac
    26041 tgcgccacga taaccgcttt catccccttc gaccacaacg aagtgttagt gtgcactgac
    26101 tcactcatcg tgttgttccg cttcgtctac ccagcttttc aacgtgacaa agtcaggttt
    26161 gccgctgcca agtaatggca tctgtttcag atagcgaata tcgcgcggta cagcaagctc
    26221 cggcacgccg tgctcgcggg catactgttg taacttatcg cgcgtcagtt cgttatctgt
    26281 ggtgaaaagc accagtgcct cgcctttgct ggcatcgctc ttaatcgcag tggcatggac
    26341 tttatctggc gaaacaccaa gtgccagttg ttccaccatt tccagcgaca ccatttcgcc
    26401 tgcaattttg gcaaagcgtt ttgcgcggcc ctgaatctgc acaaagccct gctcatcaaa
    26461 acgcacgata tcgccagtgt cataccagcc gcgctccatt tcgccgcgaa tattctcggc
    26521 ggtgggcact tccagtacac caggcttctc cacccgcaga tagccgttca ttatgttcgg
    26581 ccctttcagt tgcaggcgtc cgccctcttc gataccagga accgacaaca ggcgcgcatc
    26641 cattcctggt agaatacgcc ctaccgtacc gggtttcgcc gccatcggta cgttgataga
    26701 aacgacaggc gcgcattcgg tcacaccgta gccttcaagg atgcgcaggc caaatttatc
    26761 ctgccaaagc tgtttggtac tttcttgtag tttttctgcg cctgccacca catagcgtag
    26821 acgatagaag tcatacgggt tggcaaagcg cgcgtagtga ccgaggaaag tcgaggtacc
    26881 gaacaacacg gtgcaactgc ggtcatacac caactccggc acaatgcggt aatgcagcgg
    26941 gcttggataa aggaacactt ctgcacctgt gagcagtggc gtaaacaggc ctaccgtcag
    27001 cccaaaggag tgaaacagcg gtaacgccga cataaagcga tcgttggtgg tgaagtcggc
    27061 aatcgtttta atctgctcaa cgttcgccag aatgctttta tggctatgga cgacgccttt
    27121 cggatggcct tcagaaccgg aggtaaagag gatcagcgct tcttcttccg gctgctgttt
    27181 taactgtgcc agacgcggca tcagcaaatg agcgaaaatc cacactttgt cggcagtggt
    27241 gacatctgct tttaaatctt ccagatagac ccagcgcacc tgagtaagtt gctccggcag
    27301 atgccagagt ttgcctttat cgagaaactg acgggaagtg aagatggttt tgatttcagc
    27361 cgccgtaata gcactggtca gcccttttac cccggcggtg tagttcatca ttgcgggaat
    27421 gcggcgacgg gcgatggccc caaaaatcac tgccgcactg atgcctgcat tgggcagcat
    27481 taagccgatg cgttcgcctt caacactgta tttttcaagg atgcgtccaa caaacagcgt
    27541 tttcgtaagc aatttgcgat aggagtctgg ggtaaagttg acgtcttcga cacatttctt
    27601 cccggctccg aagcggtaca ttgcgctcag taaagattcg tacagcgttt cacgcgggcg
    27661 caccgccata cgcgcctcca tcattatttg atgcagcatt tcgccagcga ttttgcgacg
    27721 gtcacgggca cgcggcgcat ccggcatctc cacctgcgtt ggtggcaaaa tatgcagagt
    27781 aatttgcggg aacaagcgac gtttaaccag acctttcagg cggctgaagt gcgtaagttc
    27841 cgccccttca atacgcacag gaataaccgt tgcaccagac ttcgccgcga caaatcccgc
    27901 gccatcgtag attttcatca gcgagccagt ggtggtgatg cgtccttcag ggaaaatcac
    27961 cactggtcgg ccctgttcca ccagacgtac cagatgttta atcgccatag gttgcgtcgg
    28021 gtcgagagga acaaagtcga taaatgattt cagccaacgc atataccact gttggcttat
    28081 tgaggtgtaa acggcaaata ccggacgtac gggtaaaaac aatgccagca gtatgccatc
    28141 aataaaagag acgtgattag gcgtgattag aacgcgctcg cccttcagtg ccttggtgtc
    28201 acccgtaacg cgaacgcgat acaaaacacg gcacaaattt cgaaaaaagc taaaaagcat
    28261 tccaactccc tttgctctga ttcagtaaaa gcgaatggag ggagattaca cgagataaag
    28321 aacgcgagcg acagtaaatt aggtgcgaaa aaaaacctgc gcatccgcgc aggttggtgc
    28381 aagagacagg gtacgaagag cgtaccgaat aatctcacca atcaatacct ctgggatctt
    28441 gattgtggtc tgcacgacga ctcttcgcca gcgagaaaac gcaaaggaat gaagggaaat
    28501 gcaacgaggt gtgtaaattg tcggttactg ttacagattg atgaccggca aaaaaaaacc
    28561 tgcgcatctg cgcaggctgg tgtaattcat gtgctcaacc cgaagttgac ttcacctatc
    28621 aatacctctg ggatcaccac tttagcaacc tgaagccaaa cgccaccagc ggtcaatcgc
    28681 aacagcgttt cgcaaagtgt aaccaaaggt ttgcaatctc tttttttgtc ttacttattt
    28741 gcgtttggct cacattccca caatgaaaac acgccgcccc ttgaaccaac gggcgttttc
    28801 cgtaacactg aaagaatgta agcgtttacc cactaaggta ttttcatggc gaccataaag
    28861 gatgtagccc gactggcagg cgtttcagtc gccaccgttt cccgcgtcat taataattca
    28921 cccaaagcca gcgaagcttc ccgactggct gtgcatagtg caatggagtc tcttagctat
    28981 cacccgaacg ccaacgcccg tgcgctggcg cagcagacca ctgaaacgat cggtctggtc
    29041 gttggtgatg tttccgatcc gtttttcggt gcaatggtga aagcggtcga acaggtggct
    29101 tatcacactg gtaatttttt attgattggc aacggttacc acaacgaaca aaaagagcgt
    29161 caggccattg agcaactgat ccgccatcgc tgtgctgcgc tggttgtcca tgccaaaatg
    29221 atcccggatg ccgatttagc ctcattaatg aaacaaatgc ccggtatggt gctgatcaac
    29281 cgtatcctgc ctggctttga aaaccgttgt attgctctgg acgatcgtta cggtgcctgg
    29341 ctggcaacgc gtcatttaat tcagcaaggt catacccgca ttggttatct gtgctctaac
    29401 cactctattt ctgacgccga agatcgtctg caagggtatt acgatgccct tgctgaaagt
    29461 ggtattccgg ccaatgaccg gctggtgaca tttggcgaac cagacgaaag cggcggcgaa
    29521 caggcaatga ccgagctttt gggacgaggc agaaatttca ctgcggtagc ctgttataac
    29581 gattcaatgg cggcgggcgc gatgggcgtg ctcaatgata atggtattga tgtaccgggt
    29641 gagatttcgt taattggctt tgatgatgtg ctggtgtcac gctatgtgcg tccgcgcctg
    29701 accaccgtgc gttacccaat cgtgacgatg gcgacgcagg ctgccgaact ggctttggcg
    29761 ctggcggata atcgccctct cccggaaatc actaatgtct ttagtccgac gctggtacgt
    29821 cgccattcag tgtcaactcc gtcgctggag gcaagtcatc atgcaaccag cgactaaccg
    29881 cagttaaagc aattccagcg ccagtaattc ttcaatggtc tggcgacggc gaatcaaccg
    29941 cgcctgacca ttatcaaaca gaacttctgg taacagcgga cggctgttgt agttggatga
    30001 cattgacgcg ccatatgccc ctgtatcatg cagtaccaga taatcacccg ccttcacttc
    30061 cggcaaggcg cgggtttcga catttccccc ttcctgctgg gtaaagacat cgcccgattc
    30121 acataacggc ccggcgacga cggtttccac cgttggcgcg tgttccagag aacgaccatc
    30181 agctgccagg gcactgatat ggtggtaact accgtacatt gccgggcgca tcagatcgtt
    30241 gaacccggca tcaaccagaa caaagtggcg gctacccatt tgtttgacgc tccgcacttg
    30301 agtaattaat acgccagcct gcgctaccag aaagcgaccc ggttcaattt ccagtttcac
    30361 aggatggccc aaatggcggg cgatttgctc acgcgcggca ttccacagac cataataatg
    30421 ttcggtatca accgcctctt caccctgttg ataaggaata gaaagcccac cgcccgcaga
    30481 aatagcctgt aaatcctgac cgaattcgag gacttgacgc accatagcac cacatacctg
    30541 ttccagatgg gcataatcga cgccagaacc aatgtgcatg tgaatgccga ccagctgtag
    30601 atgatgacgt tgtatcacgt ccagtgcggc gggcagatcg gtgtaccaga taccgtgctt
    30661 gctgttttcg ccaccggtat tggttttttg gctatgcccg tgaccaaacc ccggattaac
    30721 acgcagccat acccgatgcc ctggcgaaac ctgccccagt tggtcgagca tatcaacaga
    30781 acccgcattc accggaattt gcaattcact gacgcgttca agcgtcgcct gatcgataac
    30841 atctgccgta aaaacaatat catcagggtg cgtttgcgga ttgtaacccg ccgccagtgc
    30901 acgctctatt tcacctaacg agacggaatc cacttttacg ccctgctcac gcattaagcg
    30961 caaaatatga atattggaac aggctttctg tgcaaagcgc accacatcaa actgtttcag
    31021 cgccgcaatc tgccgacgaa taatttgcgc atcgtagacc cacaccgggc agccaaattc
    31081 tgcgggcaaa cgcagcagat tttcggcggt gagatcggta tcagtgctga acagtgaatg
    31141 tggcataaca aactccagat aagtgctttt ttatgattac gccacatcat aaaaagaata
    31201 aaaaatatcg atttatgtcg agtctatgca aaaatgatat ggattaccgg attgcgagag
    31261 agcgctaatg gccgccgtta acttacgtca tattgaaatt tttcatgcgg taatgaccgc
    31321 cggaagcctg actgaggcgg cacacctgct acacacctca cagccaaccg tcagccgcga
    31381 actggcgcgc tttgagaagg tgatcgggct gaaattgttt gagcgcatac gtggacgatt
    31441 acatcctacc gtgcaaggac tgcgtctgtt tgaagaagtg caacgatcct ggtacggact
    31501 ggatcgcatt gtcagtgccg cagaaagtct gcgcgagttt cgccagggag aactgtctat
    31561 tgcctgcctg ccggtctttt cgcaatcttt tttaccgcag ctcctgcaac cctttctggc
    31621 acgttatccc gatgtcagct taaatatcgt gccccaggaa tcaccgctac ttgaagagtg
    31681 gctctcggcc cagcgtcatg atttaggact cactgaaacg ctccatacgc ctgcgggaac
    31741 agaacgtacc gaattactct ctttagatga agtgtgtgtg ttacctccgg gccatccgct
    31801 ggcggtaaaa aaggtattaa cgccggatga ttttcacggt gagaactaca tcagcctttc
    31861 ccgtactgac agctatcgcc agttgctgga tcaattgttt actgagaatc aggttaaacg
    31921 acgcatgatc gtagaaaccc acagcgccgc gtcagtctgc gcaatggtac gggcgggggt
    31981 aggcgtttca gtggttaacc cgctcaccgc actggattat gcggcaagcg gtttagtggt
    32041 gcggcggttc agcattgcgg ttccattcac cgtcagcctg atccgccccc tgcaccgccc
    32101 gtcatcagcg ctggtgcagg cgtttagtga gcatttacaa gcgggattac cgaaactggt
    32161 cacttctctt gacgctattt tgtcgtcagc tacgacagca taaaagcgac agcatcctcg
    32221 gcatggatcg ccgcggtatc aaacacaggc agaacactgc gctcttctgg caccagtaaa
    32281 ccaatttctg tgcagccaaa aatgacgccc tgtgcgccct gttctgcaag gcgagcaatc
    32341 acttgcgcat aataagcgcg tgacgcttcg gtaaattgcc ccagacacag ttcttcaaaa
    32401 ataatctgat taattttcgc ccggtcatcc gcttcaggaa taagacagtt gatggaaaat
    32461 tgttccgtca gccgcccgcg ataaaaatcc tgttccatgg tgtaacgtgt acccagcagc
    32521 gccacacgag tcattcctgc cccggtaatt gcacgtccag tggcatccgc tatgtgtaag
    32581 aaaggcagag agcaacgaga ctcaatggca tccgccactt tgtgcatcgt attggtgcat
    32641 aacacaatac cttctgcgcc cgcccgctgt aagccaagcg ccgcctcagc cagaatatcc
    32701 ccggttttgt cccactcacc gcgacgctgg cactcttcta tttcatgaaa atcgacgcta
    32761 tgtagcagca cttgcgcaga gtgaagccca ccaagccgct gtttaatgcc ttcatttatc
    32821 agacgatagt aaggaatagt ggattcccag ctcattcctc ccagcaaacc aattgttttc
    32881 attcctctct cctgatgagt gttaccgcaa caaggctgaa taaggaacga ggcaaacgcc
    32941 tcaacggcca ggtgcctaat ctctattaac gaaaaaaggg ccggatgtac agcacatccg
    33001 gcccgtgaaa tcagacgccg atatttctca acttttcgcc tgccatcagt ttgcgctcga
    33061 tatgttccag cgtgacattt ttggtttccg gaatgagcca gaaagtgatg cccacaaacg
    33121 caatgttcag cgcagtgtag agccagaacg taccggcagc gccaatgcta tcaagcagtg
    33181 tcaggaaggt cgcgccgata atcatattcg acacccagtt tgtcgtcgtt gagcaggtga
    33241 tgccaaaatc gcggcatttc agcggctgaa tttcagagca caagatccac accactggcg
    33301 cggcgctcat cgcataaccg gcaatacaca tcatcgtcat gccaacagag agccaggaca
    33361 agccactgga agccgtaccg ttatcaaact gcatcaggca gtaacccagc accagagtgc
    33421 ctaacgccat cacgctgaaa ccaattttca gcgccggctt acgccctgct ttatctaccg
    33481 taaagaccgc aatgaaggta gcgaacataa aggtcagccc cacgaccaga gtcgcaatca
    33541 tctgttgttc tgtggtcgta aagcccgcca ttttgaagat acgtggcgcg tagtacatga
    33601 tgatgttcat accggtaaac tgctgcatcg cctgcaacaa cataccgaga aatacagcac
    33661 gacggacgtt acggttgatc ttaaacagtg cccaaccgcc ctgttttaac ttcaggcttt
    33721 cgcgaatttc gttgagttct tctcgcgctt tttccgacgt atcgcgcagc atacgcaata
    33781 cttcttccgc ctcaatatga cgcccctttt ccgccagcca gcgcgggcta tttggcagga
    33841 agaccaccag aataatcagc agaactgctg gtaaagcaag aacccccaac attgcgcgcc
    33901 agttaccgct ataactgaac gctgtatcgg ataaaaacgc cagcacgatg ccgagagtga
    33961 ccatcaactg gtacatgctg atcatcttac cgcgaacgtt ttcacttgcc atttcagaaa
    34021 gatatagagg agcggtgtaa gacgcgatcc cgacagcaat gcccaacacc acacgagcgg
    34081 cgattaacat ctctacgctg gtcgcaaaag cggaccctat cgaaccgagt acaaacagga
    34141 tggcccccgc catcaggctg tatttacgcc ccaggcggaa cgacagccaa ccattaaaca
    34201 gcgcaccaat tgctgcaccg agcatcatgc tactgaccac ccattcctgc aaacgactgg
    34261 tcagcacaaa gtgatcggta atgaacggca acgctccagc gattacgccg atatcaagac
    34321 caaataacaa tcctgcgacc gcagcagcta ccgaaacaaa catattcata cgccgcgtat
    34381 cccgcagagg acgtggcgtt aaagcagatt ccgtattgat agtaaccatt ttttcctgcc
    34441 agcagagagt aagacatagt gaaaaaatac gtgaacaatt cacacaggtg tcaggtcgga
    34501 aatagcataa atatggatta aattgctacg acatgtcgtt atgtgatgga tattccaatt
    34561 ttcaaattaa gttgaattat tgagattatt attaaccacc taattttaaa gcagataaaa
    34621 ttcataaagt tcatcaattg ataattgata tggattattt cataaccatg atatggatta
    34681 tgatgatcta caggtataaa aaaccctgcc atgcgacagg gtcataaaag taagaggaat
    34741 gaattaacgc gccagccaac cgccatccac ggcaatggta taaccattca cataatctga
    34801 agcgctggag gcaaggaaca ccaccggccc catcaggtca ctcggcagtc cccaacggcc
    34861 agctggaatg cggtcgagaa tttccgcgct acgttgttca tctgcccgca gttgttgagt
    34921 attgttggtc gccatgtaac ccggagctat cgcattaaca ttaatgttgt gtttagccca
    34981 ttcgttcgcc atcaatcgcg tcacgcccat cacaccgctt tttgatgcgg tataagaagg
    35041 cacacggatc ccgccctgga aggagagcat tgacgcgata ttgataatct tgccgccatt
    35101 gccttgcgcg ataaagtgtt tcgccgctgc ctgagacatg aagaatacgc tcttgatatt
    35161 caggttcatg acatcgtccc agtctttttc gctgaattcg agagcgtctt cgcggcgaat
    35221 caatccggcg ttattcacca ggatatcaat atgaccaaac tccgctaccg cgcgatccag
    35281 cagtgctgga atgccatcaa tctttcgcag atcggcggtc aggcttaaaa aacgacgccc
    35341 cagcgccgtg acctgcttga tggtttcagt cggttcaacg atgttaatgc caacaatgtc
    35401 acagcccgct tgcgccagcc ccaacgccat cccctggccc agcccagtat cacaaccagt
    35461 gacgaccgca actttacctt cgagagaaaa tgcacttaaa atcataacaa taccttattc
    35521 gtttatgccc acaactagcg caaatcttta acggccacat ggtccatatc atcaaaaacc
    35581 tggttttcac cgaccatgcc ccagataaag gtataagctt tggttccgac gccggaatgg
    35641 atcgaccagc tcggggagat caccgcctgc tcgttatgca tcacaatgtg acgcgtttct
    35701 tgcggttgcc ccatcatgtg gaaaacgcag gcgtcatcat ccatattgaa atagaaataa
    35761 acttccatcc ggcgctcgtg ggtgtgacac ggcatggtgt tccacaagtt acctggagcc
    35821 agctccgtca gccccatact caattggcag gtttccagca catccgggac aaaatatttg
    35881 ttaatcgtgc gacggttact ggtgaggtta tcgcctaacg tgactggaga tacttcgtcc
    35941 ggtgtgactt ttttggtggg atacgtcgta tgcgcgggtg cgcaattgta ataaaacttc
    36001 gccggagtgg cggtatcaat actggcaaag acaacttctt ttgcaccttt accaacatac
    36061 agggcgtcgc ggtgaccgat ttcatagcat tgaccatcga cagtaatcgt accggcaccg
    36121 ccaatattga taacacctaa ctcgcgacgt tcaaggaaat agcttacgcc gagttgttta
    36181 ccaacttccc cgccaacgga aaccgtttta gttaccggca taatgccgcc aacaataata
    36241 cggtcaatgt ggctgtaaac catggtgtac tcatcggcga caaatacctt ttcaaccaaa
    36301 aattcattgc gcagcccctg ggtatccagc gtttttgcgt gcgcgctgtg gatgctctgt
    36361 cttacgtcca catcaacctc cgataatcag tgaagcgtga aaatagaaaa caataataaa
    36421 acactgtttc gttttctcta tgaggggatg atatgcccat gggatagggt tttcaattaa
    36481 aaataaaaca atgtttcatt ttcaagttaa ggattaaaaa aagtgcagtg tcgatcacga
    36541 atatgtcatg agaatggaaa aatcagggag ccctgaaaag gtatgtcgac tcaccagcca
    36601 tcattattca acagattaat gacggcagat atttatcggt aaggaggtaa tgcagagcta
    36661 ctcgtcacgt tcaatggtca atgccacacc ctggcctccg ccgatacaaa gcgttgccag
    36721 tcctttgcgg gcattacgtt tcaccatttc atgaacaaga gaaaccagga ttcggcaacc
    36781 ggaagcgcct atcgggtgac cgagtgcgat cgcaccacca ttaacattga cccgacgctc
    36841 atcccattca agcatcttgc caaccgaaag cgcctgtgca gcaaacgctt cattagcctc
    36901 gataagatcg acatcagcca actgccagcc aacacgctcc aggcaacggc gggtcgcata
    36961 caccggcgca attcccatca atgccggatc cacaccaacg ctggcaaatg cgcggatgcg
    37021 ggccagcacg ggtaaattca acgctcgtgc tttggcttcg ctcatcatca ttactgcagc
    37081 tgccccatcg tttatggatg atgcattacc cgctgtcaca gaaccgagac tatcaaatga
    37141 aggatttaaa cgggctaagc cttctgcgct ggtgtcagtg cgtggctgtt catcggtatc
    37201 aacaaccaac gtctgaccgt tactttgggt tattaccggg acgatctcat ctttaaatcg
    37261 tccggcgtca atcgccgctc gcgctttttg ttgcgagcta agtgcgtaag catcctgcaa
    37321 ctgacggctg atgccatatt cgcgagccag attttcggcg gtgacaccaa tatgataatc
    37381 attgaaggca tcccacaacc catcatgcac aagactatca accaactggc tattgccaag
    37441 ctgtgcgccg gtgcggctat cagtcagaac atgtggtgcg cggctcatgt tttcctggcc
    37501 accggcgatg acaatatcag cctcgccaca ctgtatcgcc tgagtagcca gatgcagtgc
    37561 tttaagcccg gaaccacaaa cgtcattaat agtgattgca gaaacgctat taggaagacc
    37621 acctttaata gccgattgtc ttgccggatt ctgccctgcc cctgcagtca acacctgacc
    37681 aagaatcact tcatccaccg catatgcagg aacgccggta cgttctatta acgctttgac
    37741 gaccaggctg ccaagttcca cggcggaatg acccgctaac gcaccacgaa agcagccgat
    37801 aggtgtccgt aacgccccga caatcacaac gtctttcatc acaacctcgc accaattaac
    37861 agtgcaatag taaatgattg ttaattacag ttatcttaat tgtttaaaaa aagtgaccat
    37921 tatcacaaag gaaatacgcc tgagtagtag tcagagacat aactggcacg taaggtttgc
    37981 aaccactaac ccaccaatag agaggtagat aaggcgttaa tctcccatac ttaacctggt
    38041 ttatggtaaa ttgccctcca ttttgtttaa tttgtagatg atacgttcag ataatgtcta
    38101 atatttggtc aaaagaagaa actctgtgga gtttcgcact ctacggcaca gccgttggtg
    38161 caggcacgct cttccttcct attcagttag gttcggcggg ggctgtggtc ctgtttatta
    38221 ctgctctggt cgcctggcct ttaacatatt ggccacataa agccttatgc cagttcatcc
    38281 tctcatcgaa aacatcagca ggtgaaggga taacgggcgc ggtaacacac tactatggca
    38341 agaagattgg taatctgatt accacgctgt acttcatcgc cttttttgtc gtcgtgttga
    38401 tatatgcagt ggcaattacc aactcactta cggaacagct ggcaaagcat atggttattg
    38461 atcttcgcat ccgtatgcta gtgagtctgg gggttgtatt aatcctgaat ctcatttttc
    38521 tgatgggacg ccatgccact attcgggtaa tgggattttt ggtattccca ttgattgcct
    38581 atttcttatt tctttctatt tacctggtcg gtagttggca acctgatcta ttaacaaccc
    38641 aggtagagtt caatcagaat acccttcacc agatatggat atcgattccc gtgatggttt
    38701 tcgcttttag ccatacgccc attatttcta cgtttgccat agacagacgt gaaaaatatg
    38761 gcgaacatgc tatggataaa tgcaaaaaaa ttatgaaagt cgcttatctc atcatctgca
    38821 taagtgtatt gttctttgtc tttagctgcc tgctttctat tccaccttcg tatattgaag
    38881 cggctaaaga agaaggggtt accattttat cggcgctttc tatgctgccg aacgccccag
    38941 catggttgtc aatttccggg attattgtcg cagtagttgc gatgtcgaaa tcattcctgg
    39001 gtacgtactt tggcgttatt gaaggtgcca cagaggtcgt caaaacaaca ttacagcagg
    39061 ttggtgtaaa gaaaagtcgt gcatttaacc gcgcactatc aattatgttg gtatcgctga
    39121 ttaccttcat tgtttgttgc attaacccga acgcgatttc gatgatttac gcgatcagcg
    39181 gcccgctcat tgccatgata cttttcatca tgcctacgct gtcaacgtat ctcatcccgg
    39241 cgcttaaacc ctggcgttcc atcggaaatc tgattaccct gatcgtgggt atcctgtgcg
    39301 tatcggtaat gttctttagc taatatccgt tgtggatggt tcaaaattgc ttcaattttg
    39361 aaccatccca gttttca
//
LOCUS       DAHHTG010000033        39037 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.033, whole genome shotgun sequence.
ACCESSION   DAHHTG010000033 DAHHTG010000000
VERSION     DAHHTG010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 39037)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39037)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39037
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.033"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            complement(80..1264)
                     /locus_tag="NBI74_001662"
     CDS             complement(80..1264)
                     /locus_tag="NBI74_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001218899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCD1932021.1"
                     /translation="MALTDIKVKTAKPKDKPYKLADGGGMYLLINTNGSKYWRMKYRF
                     AGKEKMLSIGVYPDVTLADAREKRSEARKLLAAGGDPGEAKKEEKIAQQMSLQNTFEA
                     IAREWHQSKADRWSLRYRDEIIDTFEKDIFPYIGKRPIAEIKPMELLEALRKMEKRGA
                     LEKMRKVRQRCGEVFRYAIVTGRADYNPAPDLASALATPKKVHFPFLTANELPHFLND
                     LAGYTGSIITKTATQIIMLTGVRTQELRFARWEDIDFETKLWEIPAEVMKMKRPHIVP
                     LSEQVIMLFKQLEPISKHHPLVFIGRNDPRKPISKESINQVIELLGYKGRLTGHGFRH
                     TMSTILHEQGFNSAWIEMQLAHVDKNSIRGTYNHALYLDGRREMMQWYADYIDSLSSR
                     ES"
     gene            complement(1564..1658)
                     /locus_tag="NBI74_001663"
     tRNA            complement(1564..1658)
                     /locus_tag="NBI74_001663"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1622..1624),aa:Sec,seq:tca)
     gene            1951..3333
                     /locus_tag="NBI74_001664"
     CDS             1951..3333
                     /locus_tag="NBI74_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418114.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide family
                     transporter"
                     /protein_id="HCD1932022.1"
                     /translation="MKSEVLSVKEKIGYGMGDAASHIIFDNVMLYMMFFYTDIFGIPA
                     GFVGTMFLVARALDAISDPCMGLLADRTRSRWGKFRPWVLFGALPFGIVCVLAYSTPD
                     LSMNGKMIYAAITYTLLTLLYTIVNIPYCALGGVITNDPTQRISLQSWRFVLATAGGM
                     LSTVLMMPLVNLIGGDNKPLGFQGGIAVLYVVAFMMLAFCFFTTKERVEAPPTTTSMR
                     DDLRDIWQNDQWRIVGFLTIFNILAVCVRGGAMMYYVTWILGTPEVFVAFLTTYCVGN
                     LIGSALAKPLTDWKCKVTIFWWTNALLAVISLAMFFVPMQASITMFVFIFVIGVLHQL
                     VTPIQWVMMSDTVDYGEWCNGKRLTGISFAGTLFVLKLGLAFGGALIGWMLAYGGYDA
                     AEKAQNSATISIIIALFTIVPAICYLLSAIIAKRYYSLTTHNLKTVMEQLAQGKRRCQ
                     QQFTSQEVQN"
     gene            3343..5661
                     /gene="yicI"
                     /locus_tag="NBI74_001665"
     CDS             3343..5661
                     /gene="yicI"
                     /locus_tag="NBI74_001665"
                     /EC_number="3.2.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-xylosidase"
                     /protein_id="HCD1932023.1"
                     /translation="MKISDGNWLIQPGLNLIHPLQVFEVEQQGNEMVVYAAPRDVRER
                     TWQLDTPLFTLRFFSPQEGIVGVRIEHFQGALNNGPHYPLNILQDVKVTIENTERYAE
                     FKSGNLSARVSKGEFWSLDFLRNGERITGSQVKNNGYVQDTNNQRNYMFERLDLGVGE
                     TVYGLGERFTALVRNGQTVETWNRDGGTSTEQAYKNIPFYVTNRGYGVLVNHPQCVSF
                     EVGSEKVSKVQFSVESEYLEYFVIDGPTPKAVLDRYTRFTGRPALPPAWSFGLWLTTS
                     FTTNYDEATVNSFIDGMAERNLPLHVFHFDCFWMKAFQWCDFEWDPLTFPDPEGMIRR
                     LKAKGLKICVWINPYIGQKSPVFKELQEKGYLLKRPDGSLWQWDKWQPGLAIYDFTNP
                     DACKWYADKLKGLVAMGVDCFKTDFGERIPTDVQWFDGSDPQKMHNHYAYIYNELVWN
                     VLKDTVGEEEAVLFARSASVGAQKFPVHWGGDCYANYESMAESLRGGLSIGLSGFGFW
                     SHDIGGFENTAPAHVYKRWCAFGLLSSHSRLHGSKSYRVPWAYDGESCDVVRFFTQLK
                     CRMMPYLYREAARANARGTPMMRAMMMEFPDDPACDYLDRQYMLGDNVMVAPVFTETG
                     DVQFYLPEGRWTHLWHNDELDGSRWHKQQHSFLSLPVYVRDSTLLALGNNDQRPDYAW
                     HEGTAFHLFNLQDGHEAVCEVPDADGSVIFTLKAARTGNTITVTGTGEAKNWTLCLRN
                     IVKVNGLQGGSQAESEQGLVVTPQGNALTITL"
     gene            5956..7509
                     /locus_tag="NBI74_001666"
     CDS             5956..7509
                     /locus_tag="NBI74_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HCD1932024.1"
                     /translation="MQMITSRQNRLLRFLLPRREYTTIVTIAGYLNVSEKTIQRDLRL
                     LEQWLGQWRISVEKRAGAGVMLSAENIADLLHLDHLLGAECEDVDGVMNNARRVKIAS
                     QLLSETPNETSISKLSERYFISGASIVNDLRVIESWLAPLGLSLIRSPSGTHIEGSEG
                     QVRQAMALLINGIINHNEPQGVVYSRLDPGSYKALVHYFGEEEVLFVQSLLLDMENEL
                     SWSLGEPYYVNIFTHILIMMYRNTHGNALSREEDQTRQYDENIFNVASQMIHKIEQRI
                     AHTLPDDEVWFIYQYIISSGVAIDGQKDVSIISHMQASNEARLITWRLITVFSDIVDC
                     DFSEDSALYDGLLVHIKPLINRLNYRIHIRNPLLEDIKAELADVWRLTQYVVNQVFKT
                     WGKNAVSEDEVGYLTVHFQAAMERQIARKRVLLVCSTGIGTSHLLKSRILRAFPEWTI
                     VDVISAANLSQVLPDNIELIISTINLPTVTMPVAYVTAFFNDADIKRVTEMVITEKLH
                     HATSRVVEI"
     gene            7536..8006
                     /locus_tag="NBI74_001667"
     CDS             7536..8006
                     /locus_tag="NBI74_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007370547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HCD1932025.1"
                     /translation="MDITKILNTNRVILDMQATNKAEAIEELTNLLKKDGAIDCRETF
                     IKDVWQREAEGSTGFENHIAIPHGKSSAVINTTLAIGRTRQDIPWETLDGSSVRCIIL
                     FAVRLEDQNTTHIRLLSQVAGALADDDIIEQLLVETSPQKIIDLFSQYAQSDVC"
     gene            8024..8338
                     /locus_tag="NBI74_001668"
     CDS             8024..8338
                     /locus_tag="NBI74_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001028073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HCD1932026.1"
                     /translation="MNIVCVAACTAGIAHTYIAREKLIKGAKALGHTIKVETQGTIGT
                     ENELQAADISAADVVILAVDVKIKGEERFTNKRIVRVKTEIVIKSPVQFLEKVEKSLG
                     NK"
     gene            8363..9454
                     /locus_tag="NBI74_001669"
     CDS             8363..9454
                     /locus_tag="NBI74_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HCD1932027.1"
                     /translation="MKDNKIPVSQEIKKHLLTGISWMIPLIVAAGICIALGQVLGGTD
                     VGEKTGTIPWMLNQIGGWGMGLIVPLISAAIAYSIADRPGFAPGLIVGFLCGQIHTGF
                     IGGMLGGFLVGYTILLLKRYIRLPQSMQGLMPIMVLPVLSTIIGGLLMMTLIGKPIAW
                     LQEALIHLLESMQGGSRFLMGAILGAMATFDFGGPVNKTMSLFSDGLLVSGVYGPEAV
                     KFVGSIIPPFGITLSFLLTRHKYTRAEREALKAAFPMGICMITEGVIPIAARDLLRVV
                     GSCVVASAVAGGLIMVWGVESPVPHGGMFVVPLFTHPLLFCLALGIGTVICGVMLSLW
                     KKPVTERDEEFDELSDQKLKDEEITFTLE"
     gene            9466..10317
                     /locus_tag="NBI74_001670"
     CDS             9466..10317
                     /locus_tag="NBI74_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000457126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase family
                     protein"
                     /protein_id="HCD1932028.1"
                     /translation="MFADMKSMVMKAWHEHYALLAINCMNLESAHAAIRVAEKNRAPI
                     ILNLYQGHLAHFPAPVAAAVVKTLAESASVPVALALDHGKNPDCIRQAFRAGFSGLMI
                     DASAFPLEENVRQTKAVVELAASAQLCVEGELGHLADAPRYDQAANADLMTQPTDVEP
                     FIAQTGIDLLAVSVGTAHGMYAPGVVPAIDFQRLAEISRCSSVPLALHGGSGTPFDQL
                     QLCTTLGVAKINVGAAIFERGKSALLHTLCHDISIELVDALKAMELAFEEAITPYLQA
                     SGSIGKA"
     gene            10398..11258
                     /locus_tag="NBI74_001671"
     CDS             10398..11258
                     /locus_tag="NBI74_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006687642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HCD1932029.1"
                     /translation="MLVSMKELLQPTRQHGFAIGAFNVADNCFLRAVVDEAEATNTPA
                     IIAIHPSEHDFVGDAFFAHVREITQRSPVPFVLHLDHGASVEHVLRAIRCGFTSVMID
                     GSLLPYEENVALTAEVVRLAHAVGVSVEGELGTIGQTGTSVEGGVSQVTYTDPAQAAD
                     FIARTGADTLAVAIGTAHGIYPKGMQPKLQMDILRDIAGRIDIPLVLHGGSANPDAEI
                     AESVTFGVGKINISSDMKYAYFQKVREILAKESWWDPNVIYPDAINAAREVIRHKMKL
                     FGSLGKASLY"
     gene            11306..12238
                     /locus_tag="NBI74_001672"
     CDS             11306..12238
                     /locus_tag="NBI74_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HCD1932030.1"
                     /translation="MHYLGLDIGGTKIAAVVMDAHGWEIRRYRCPTQKSTYQQFVSCV
                     VALIEQIRRDVQRPMLTGIALPGSISPLTGLIKNANIQVINGHALQADLQQLLGQPVV
                     IANDGNCFALSEACDGAGQDYDVVFGITLGTGCGGGIAIKQRPFIGAWGNAAECGHIT
                     LPGYTEQEDGQSVSCYCGKHNCVESFVSGSGFSERYQQMTGNLLTPAAIVTLAQRGDA
                     CAMQQVARFRQQLARTLATIVNVVDPGVIVIGGGLSNVELLITDLNAEVAPLVFTDQF
                     TTPIVKAQHGDSSGMRGAAWLAMRNGEANETFTN"
     gene            12219..12899
                     /locus_tag="NBI74_001673"
     CDS             12219..12899
                     /locus_tag="NBI74_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lyxose/D-mannose family sugar isomerase"
                     /protein_id="HCD1932031.1"
                     /translation="MKRSQINYVIDKAHAIAQTFRVCLPEFAYFTVDDWLQRERDNWQ
                     EVVDLQLGWDITDFGRGDFNQTGLTLLTMRNGALGSAAYPKPYAEKMLQIQQDQQTPW
                     HFHTHKMEDILNRGGGDLCMQLAWANEANLCDDQRVITVSVDGQRRTMKPGETLVLKP
                     GQGICLPPRLYHRFWAEKAFVLGWEISMVNDDQHDNYFLEPGGRFPAIEEDEPVKWLL
                     CSEYGILR"
     gene            complement(12943..14652)
                     /locus_tag="NBI74_001674"
     CDS             complement(12943..14652)
                     /locus_tag="NBI74_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HCD1932032.1"
                     /translation="MKFIGKLLLYIFIALLVVIAGLYFLLQTRWGAEHISAWVSENSD
                     YHLAFGAMDHRFSAPSHIVLENVTFGRDGQPATLVAKSVDIALSSRQLTEPRHVDTIL
                     LENGTLNLTDQTAPLPFKADRLQLRDMAFNSPNSEWKLSAQRVNGGVVPWSPEAGKVL
                     GTKAQIQFSAGSLSLNDVPATNVLIEGSIDNDRVTLTNLGADIARGTLTGNAQRNADG
                     SWQVENLRLADIRLQSEKSLTDFFAPLRSVPSLQIGRLEVIDARLQGPDWAVTDLDLS
                     LRNMTFSKDDWQTQEGKLSMNASEFIYGSLHLFDPIINAEFSPQGVALRQFTSRWEGG
                     MVRTSGNWLRDGKMLILDDAAIAGLEYTLPKNWQQLWMETTPGWLNSLQLKRFSASRN
                     LIIDIAPDFPWQLTALDGYGANLTLVTDHKWGVWSGSANLNAAAATFNRVDVRRPSLA
                     LTANSSTVNISELSAFTEKGILEATASVSQTPQRQTHISLNGRGVPVNILQHWGWPEL
                     PLTGDGNIQLTASGDIQVNVPLKPTVSGQLHAVNAAKQQVTQTMNAGVVSSGEVTSTE
                     PVQ"
     gene            complement(14773..16164)
                     /gene="xanP"
                     /locus_tag="NBI74_001675"
     CDS             complement(14773..16164)
                     /gene="xanP"
                     /locus_tag="NBI74_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine/proton symporter XanP"
                     /protein_id="HCD1932033.1"
                     /translation="MSVSTLESENAQPVVQTQNSELIYRLEDRPPLPQTLFAACQHLL
                     AMFVAVITPALLICQALGLPAQDTQHIISMSLFASGVASIIQIKAWGPVGSGLLSIQG
                     TSFNFVAPLIMGGTALKTGGADVPTMMAALFGTLMLASCTEMVISRVLHLARRIITPL
                     VSGVVVMIIGLSLIQVGLTSIGGGYAAMSDNTFGAPKNLLLAGVVLALIILLNRQRNP
                     YLRVASLVIAMAAGYALAWFMGMLPESNEPMTQELIMVPTPLYYGLGIEWSLLLPLML
                     VFMITSLETIGDITATSDVSEQPVSGPLYMKRLKGGVLANGLNSFVSAVFNTFPNSCF
                     GQNNGVIQLTGVASRYVGFVVALMLIVLGLFPAVSGFVQHIPEPVLGGATLVMFGTIA
                     ASGVRIVSREPLNRRAILIIALSLAVGLGVSQQPLILQFAPEWLKNLLSSGIAAGGIT
                     AIVLNLIFPPEKQ"
     gene            16444..17649
                     /gene="gltS"
                     /locus_tag="NBI74_001676"
     CDS             16444..17649
                     /gene="gltS"
                     /locus_tag="NBI74_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HCD1932034.1"
                     /translation="MFHLDTLATLVAATLTLLLGRKLVHSVSFLKKYTIPEPVAGGLL
                     VALALLILKKSMGWEVNFDMSLRDPLMLAFFATIGLNANLASLRAGGRVVGVFLIVVV
                     GLLVMQNAIGIGMASLLGLDPLMGLLAGSITLSGGHGTGAAWSKLFIERYGFTNATEV
                     AMACATFGLVLGGLIGGPVARYLVKHSTTPNGIPDDQEVPTAFEKPDVGRMITSLVLI
                     ETIALIAICLTVGKIVAQLLAGTAFELPTFVCVLFVGVILSNGLSMMGFYRVFERAVS
                     VLGNVSLSLFLAMALMGLKLWELASLALPMLAILVVQTIFMALYAIFVTWRMMGKNYD
                     AAVLAAGHCGFGLGATPTAIANMQAITERFGPSHMAFLVVPMVGAFFIDIVNALVIKL
                     YLMLPIFAG"
     gene            complement(17683..19764)
                     /gene="recG"
                     /locus_tag="NBI74_001677"
     CDS             complement(17683..19764)
                     /gene="recG"
                     /locus_tag="NBI74_001677"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HCD1932035.1"
                     /translation="MKGRLLDAVPLSSLTGVGAALSNKLAKINLHTVQDLLLHLPLRY
                     EDRTHLYPIGELLPGVYATVEGEVLNCNISFGGRRMMTCQISDGSGILTMRFFNFNAA
                     MKNSLATGRRVLAYGEAKRGKYGAEMIHPEYRVQGDLSTPELQETLTPVYPTTEGVKQ
                     ATLRKLTDQALDLLDTCAIEELLPPELSQGMMTLPEALRTLHRPPPTLQLSDLETGQH
                     PAQRRLILEELLAHNLSMLALRAGAQRFHAQPLSANDALKNKLLAALPFKPTGAQARV
                     VAEIERDMALDVPMMRLVQGDVGSGKTLVAALAALRAVAHGKQVALMAPTELLAEQHA
                     NNFRNWFAPLGIEVGWLAGKQKGKARLAQQEAIASGQVQMIVGTHAIFQEQVQFNGLA
                     LVIIDEQHRFGVHQRLALWEKGQQQGFHPHQLIMTATPIPRTLAMTAYADLDTSVIDE
                     LPPGRTPVTTVAIPDTRRTDIIDRVRHACMTEGRQAYWVCTLIEESELLEAQAAEATW
                     EELKLALPELNVGLVHGRMKPAEKQAVMTSFKQGELHLLVATTVIEVGVDVPNASLMI
                     IENPERLGLAQLHQLRGRVGRGAVASHCVLLYKTPLSKTAQIRLQVLRDSNDGFVIAQ
                     KDLEIRGPGELLGTRQTGNAEFKVADLLRDQAMIPEVQRLARHIHERYPQQAKALIER
                     WMPETERYSNA"
     gene            complement(19770..20459)
                     /gene="trmH"
                     /locus_tag="NBI74_001678"
     CDS             complement(19770..20459)
                     /gene="trmH"
                     /locus_tag="NBI74_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(18)-2'-O)-methyltransferase
                     TrmH"
                     /protein_id="HCD1932036.1"
                     /translation="MNPTRYARICEMLARRQPDLTVCMEQVHKPHNVSAIIRTADAVG
                     VHEVHAVWPGSRMRTMASAAAGSNSWVQVKTHRTIGDAVAHLKGQGMQILATHLSDNA
                     VDFREIDYTRPTCILMGQEKTGITQEALALADQDIIIPMIGMVQSLNVSVASALILYE
                     AQRQRQNAGMYLRENSMLPEAEQQRLLFEGGYPVLAKVAKRKGLPYPHVNQQGEIEAD
                     ADWWSTMQAAG"
     gene            complement(20466..22574)
                     /gene="spoT"
                     /locus_tag="NBI74_001679"
     CDS             complement(20466..22574)
                     /gene="spoT"
                     /locus_tag="NBI74_001679"
                     /EC_number="3.1.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional GTP
                     diphosphokinase/guanosine-3',5'-bis pyrophosphate
                     3'-pyrophosphohydrolase"
                     /protein_id="HCD1932037.1"
                     /translation="MYLFESLNQLIQNYLPEDQIKRLRQAYLVARDAHEGQTRSSGEP
                     YITHPVAVACILAEMKLDYETLMAALLHDVIEDTPATYQDMEQLFGKSVAELVEGVSK
                     LDKLKFRDKKEAQAENFRKMIMAMVQDIRVILIKLADRTHNMRTLGSLRPDKRRRIAR
                     ETLEIYSPLAHRLGIHHIKTELEELGFEALYPNRYRVIKEVVKAARGNRKEMIQKILS
                     EIEGRLQEAGIPCRVSGREKHLYSIYCKMVLKEQRFHSIMDIYAFRVIVNDSDTCYRV
                     LGQMHSLYKPRPGRVKDYIAIPKANGYQSLHTSMIGPHGVPVEVQIRTEDMDQMAEMG
                     VAAHWAYKEHGETSTTAQIRAQRWMQSLLELQQSAGSSFEFIESVKSDLFPDEIYVFT
                     PEGRIVELPAGATPVDFAYAVHTDIGHACVGARVDRQPYPLSQPLTSGQTVEIITAPG
                     ARPNAAWLNFVVSSKARAKIRQLLKNLKRDDSVSLGRRLLNHALGGSRKLNEIPQENI
                     QRELDRMKLATLDDLLAEIGLGNAMSVVVAKNLQHGDASIPPATQSHGHLPIKGADGV
                     LITFAKCCRPIPGDPIIAHVSPGKGLVIHHESCRNIRGYQKEPEKFMAVEWDKETAQE
                     FITEIKVEMFNHQGALANLTAAINTTTSNIQSLNTEEKDGRVYSAFIRLTARDRVHLA
                     NIMRKIRVMPDVIKVTRNRN"
     gene            complement(22593..22868)
                     /gene="rpoZ"
                     /locus_tag="NBI74_001680"
     CDS             complement(22593..22868)
                     /gene="rpoZ"
                     /locus_tag="NBI74_001680"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013511051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HCD1932038.1"
                     /translation="MARVTVQDAVEKIGNRFDLVLVAARRARQMQVGGKDPLVPEEND
                     KTTVIALREIEEGLINNQILDVRERQEQQEQEAAELQAVTAIAEGRR"
     gene            complement(22923..23546)
                     /gene="gmk"
                     /locus_tag="NBI74_001681"
     CDS             complement(22923..23546)
                     /gene="gmk"
                     /locus_tag="NBI74_001681"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HCD1932039.1"
                     /translation="MAQGTLYIVSAPSGAGKSSLIQALLKTQPLYDTQVSVSHTTRQP
                     RPGEVHGEHYFFVNHDEFKEMISRDAFLEHAEVFGNYYGTSREAIEQVLATGVDVFLD
                     IDWQGAQQIRQKMPHARSIFILPPSKIELDRRLRGRGQDSEEVIAKRMAQAVAEMSHY
                     AEYDYLIVNDDFDTALTDLKTIIRAERLRMSRQKQRHDALISKLLAD"
     gene            23804..25486
                     /gene="ligB"
                     /locus_tag="NBI74_001682"
     CDS             23804..25486
                     /gene="ligB"
                     /locus_tag="NBI74_001682"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigB"
                     /protein_id="HCD1932040.1"
                     /translation="MKVWMAILISILCWQSSVWAVCPAWSPARAQEEISRLQQQIKQW
                     DDDYWKEGKSEVEDGVYDQLSARLTQWQRCFGNETRDVMIPPLNGAVMHPVAHTGVRK
                     MADKNALSLWMRERSDLWVQPKVDGVAVTLVYRDGKLNKAISRGNGLKGEDWTQKVRL
                     ISAVPQTVSGPLANSTLQGEIFLKRKGHIQQQMGGINARAKVAGLMMRQGNSDTLNSL
                     AVFVWAWPDGPHLMTDRLKDLATAGFTLTQTYTRAVKNADEVAHVRNEWWKAKLPFVT
                     DGVVVRAAKEPESRHWLPGQAEWLVAWKYQPVAQVAEVKAIQFAVGKSGKISVVASLV
                     PVMLDDKKVQRVNIGSVRRWQEWDIAPGDQILVSLAGQGIPRIDDVVWRGAERTKPTP
                     PENRFNSLTCYFASDVCQEQFISRLVWLGSKQVLGLDGIGEAGWRALHQTHRFEHIFS
                     WLLLTPEQLQNTPGIAKSKSAQLWHQFNLARQQPFTRWVMAMGIPLTRAALNASDERS
                     WSQLLFSTEQFWQQLPGTGSGRARQVIEWKENAQIKKLGSWLAAQQITGFEP"
     gene            complement(25483..26100)
                     /locus_tag="NBI74_001683"
     CDS             complement(25483..26100)
                     /locus_tag="NBI74_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HCD1932041.1"
                     /translation="MLLHILYLVGITAEAMTGALAAGRRRMDTFGVIIIATATAIGGG
                     SVRDILLGHYPLGWVKHPEYVIIVATAAVLTTIVAPVMPYLRKVFLVLDALGLVVFSI
                     IGAQVALDMGHGPIIAVVAAVTTGVFGGVLRDMFCKRIPLVFQKELYAGVSFASAVLY
                     IALQHYVSNHDVVIISTLVFGFFARLLALRLKLGLPVFYYSHEGH"
     gene            complement(26352..27215)
                     /locus_tag="NBI74_001684"
     CDS             complement(26352..27215)
                     /locus_tag="NBI74_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HCD1932042.1"
                     /translation="MIRSMTAYARREIKGEWGSATWEMRSVNQRYLETYFRLPEQFRS
                     LEPVVRERIRSRLTRGKVECTLRYEPDVSAQGELILNEKLAKQLVTAANWVKMQSDEG
                     EINPVDILRWPGVMAAQEQDLDAIAAEILAALDGTLDDFIIARETEGQALKVLIEQRL
                     EGVTAEVVKVRAHMPEILQWQRERLVAKLEDAQVQLENNRLEQELVLLAQRIDVAEEL
                     DRLEAHVKETYNILKKKEAVGRRLDFMMQEFNRESNTLASKSINAEVTNSAIELKVLI
                     EQMREQIQNIE"
     gene            27342..28058
                     /gene="rph"
                     /locus_tag="NBI74_001685"
     CDS             27342..28058
                     /gene="rph"
                     /locus_tag="NBI74_001685"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006803115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HCD1932043.1"
                     /translation="MRPAGRSNNQVRPVTLTRNYTKHAEGSVLVEFGDTKVLCTASIE
                     EGVPRFLKGQGQGWITAEYGMLPRSTHTRNAREAAKGKQGGRTMEIQRLIARALRAAV
                     DLKALGEFTITLDCDVLQADGGTRTASITGACVALADALQKLVENGKLKTNPMKGMVA
                     AVSVGIVNGEAVCDLEYVEDSAAETDMNVVMTEDGRIIEVQGTAEGEPFTHEELLTLL
                     ALARGGIESIVATQKAALAN"
     gene            28124..28765
                     /gene="pyrE"
                     /locus_tag="NBI74_001686"
     CDS             28124..28765
                     /gene="pyrE"
                     /locus_tag="NBI74_001686"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HCD1932044.1"
                     /translation="MKPYQRQFIEFALSKQVLKFGEFTLKSGRKSPYFFNAGLFNTGR
                     DLALLGRFYAEALVDSGIEFDLLFGPAYKGIPIATTTAVALAEHHDLDLPYCFNRKEA
                     KDHGEGGNLVGSALQGRVMLVDDVITAGTAIRESMEIIQANGATLAGVLISLDRQERG
                     RGEISAIQEVERDYNCKVISIITLKDLIAYLEEKPEMAEHLAAVKAYREEFGV"
     gene            complement(28802..29398)
                     /gene="slmA"
                     /locus_tag="NBI74_001687"
     CDS             complement(28802..29398)
                     /gene="slmA"
                     /locus_tag="NBI74_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion factor SlmA"
                     /protein_id="HCD1932045.1"
                     /translation="MAEKQTAKRNRREEILQSLALMLESSDGSQRITTAKLAASVGVS
                     EAALYRHFPSKTRMFDSLIEFIEDSLITRINLILKDEKDTTARLRLIVLLLLGFGERN
                     PGLTRILTGHALMFEQDRLQGRINQLFERIEAQLRQVLREKRMREGEGYTTDETLLAS
                     QLLAFCEGMLSRFVRSEFKYRPTDDFDARWPLIAAQLQ"
     gene            complement(29505..29963)
                     /gene="dut"
                     /locus_tag="NBI74_001688"
     CDS             complement(29505..29963)
                     /gene="dut"
                     /locus_tag="NBI74_001688"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000976070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HCD1932046.1"
                     /translation="MMKKIDVKILDPRVGKEFPLPTYATSGSAGLDLRACLDDAVELA
                     PGDTTLVPTGLAIHIADPSLAAMMLPRSGLGHKHGIVLGNLVGLIDSDYQGQLMISVW
                     NRGQDSFTIQPGERIAQMIFVPVVQAEFNLVEDFDATDRGEGGFGHSGRQ"
     gene            complement(29941..31161)
                     /gene="coaBC"
                     /locus_tag="NBI74_001689"
     CDS             complement(29941..31161)
                     /gene="coaBC"
                     /locus_tag="NBI74_001689"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418096.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HCD1932047.1"
                     /translation="MSLAGKKIVLGVSGGIAAYKTPELVRRLRDRGADVCVAMTEAAK
                     AFITPLSLQAVSGYPVSDSLLDPAAEAAMGHIELGKWADLVILAPATADLIARVAAGM
                     ANDLVSTICLATPAPVAVLPAMNQQMYRAAATQHNLEVLASRGLLIWGPDSGSQACGD
                     IGPGRMLDPLTIVDMAVAHFSPVNDLKHLNIMITAGPTREPLDPVRYISNHSSGKMGF
                     AIAAAAARRGANVTLVSGPVSLPTPPFVNRVDVMTALEMEAAVNASVQQQNIFIGCAA
                     VADYRAATVAPEKIKKQATQGDELTIKMVKNPDIVAGVAALKDHRPYVVGFAAETNNV
                     EEYARQKRIRKNLDLICANDVSQPTQGFNSDNNALHLFWQDGDKVLPLERKELLGQLL
                     LDEIVTRYDEKNRR"
     gene            31333..32001
                     /gene="radC"
                     /locus_tag="NBI74_001690"
     CDS             31333..32001
                     /gene="radC"
                     /locus_tag="NBI74_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HCD1932048.1"
                     /translation="MKNNVQLLMPREKMLKFGISALTDVELLALFLRTGTRGKDVLTL
                     AKEMLENFGSLYGLLTSEYEQFSGVHGIGVAKFAQLKGIAELARRYYNVRMREESPLL
                     SPEMTREFLQSQLTGEEREIFMVIFLDSQHRVITHSRLFSGTLNHVEVHPREIIREAI
                     KINASALILAHNHPSGCAEPSKADKLITERIIKSCQFMDLRVLDHIVIGRGEYVSFAE
                     RGWI"
     gene            32218..32454
                     /gene="rpmB"
                     /locus_tag="NBI74_001691"
     CDS             32218..32454
                     /gene="rpmB"
                     /locus_tag="NBI74_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008457442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HCD1932049.1"
                     /translation="MSRVCQVTGKRPVTGNNRSHALNATKRRFLPNLHSHRFWVESEK
                     RFVTLRVSAKGMRVIDKKGIDTVLAELRARGEKY"
     gene            32475..32642
                     /gene="rpmG"
                     /locus_tag="NBI74_001692"
     CDS             32475..32642
                     /gene="rpmG"
                     /locus_tag="NBI74_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HCD1932050.1"
                     /translation="MAKGIREKIKLVSSAGTGHFYTTTKNKRTKPEKLELKKFDPVVR
                     QHVIYKEAKIK"
     gene            32740..33549
                     /gene="mutM"
                     /locus_tag="NBI74_001693"
     CDS             32740..33549
                     /gene="mutM"
                     /locus_tag="NBI74_001693"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HCD1932051.1"
                     /translation="MPELPEVETSRRGIEPHLVGATILHAVVRNGRLRWPVSEEIYRL
                     SDQPVLSVQRRAKYLLLELPEGWIIIHLGMSGSLRILPEELPPEKHDHVDLVMSNGKV
                     LRYTDPRRFGAWLWTKELEGHNVLAHLGPEPLSDDFNGEYLHQKCAKKKTAIKPWLMD
                     NKLVVGVGNIYASESLFAAGIHPDRLASSLSLAECELLARVIKAVLLRSIEQGGTTLK
                     DFLQSDGKPGYFAQELQVYGRKGEPCRVCGTPIVATKHAQRATFYCRQCQK"
     gene            complement(33588..34067)
                     /gene="coaD"
                     /locus_tag="NBI74_001694"
     CDS             complement(33588..34067)
                     /gene="coaD"
                     /locus_tag="NBI74_001694"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HCD1932052.1"
                     /translation="MQKRAIYPGTFDPITNGHIDIVTRASQMFDHVILAIAASPSKKP
                     MFTLEERVELAQQATAHLENVEVVGFSDLMANFARNQHATVLIRGLRAVADFEYEMQL
                     AHMNRHLMPELESVFLMPSKEWSFISSSLVKEVARHQGDVTHFLPENVHQALMAKLA"
     gene            complement(34075..35352)
                     /gene="waaA"
                     /locus_tag="NBI74_001695"
     CDS             complement(34075..35352)
                     /gene="waaA"
                     /locus_tag="NBI74_001695"
                     /EC_number="2.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) 3-deoxy-D-manno-octulosonic acid
                     transferase"
                     /protein_id="HCD1932053.1"
                     /translation="MLELLYTALLYLIQPLIWIRLWVRGRKAPAYRKRWGERYGFYRH
                     PLKPGGIMLHSVSVGETLAAIPLVRALRHRYPDLPITVTTMTPTGSERVQSAFGKDVQ
                     HVYLPYDLPDALNRFLNKVDPKLVLIMETELWPNLIAALHKRKIPLVIANARLSARSA
                     AGYAKLGKFVRRLLRRITLIAAQNEEDGARFVALGAKNNQVTVTGSLKFDISVTPQLA
                     AKAVTLRRQWAPHRPVWIATSTHEGEESVVIAAHQALLQQFPNLLLILVPRHPERFPD
                     AINLVRQAGLSYITRSSGEVPSTSTQVVVGDTMGELMLLYGIADLAFVGGSLVERGGH
                     NPLEAAAHAIPVLMGPHTFNFKDICARLEQASGLITVTDATTLAKEVSSLLTDADYRS
                     FYGRHAVEVLYQNQGALQRLLQLLEPYLPPKTH"
     gene            35766..36824
                     /gene="rfaQ"
                     /locus_tag="NBI74_001696"
     CDS             35766..36824
                     /gene="rfaQ"
                     /locus_tag="NBI74_001696"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptosyltransferase
                     RfaQ"
                     /protein_id="HCD1932054.1"
                     /translation="MDKPFRRILLIKMRFHGDMLLTTPVISSLKKNYPDAKIDVLLYQ
                     DTIPILSENPEINALYGIKNKKAKASEKIANFFHLIKVLRANKYDLIVNLTDQWMVAI
                     LVRLLNARVKISQDYHHRQSAFWRNSFTHLVPLQGGNVVESNLSVLTPLGLESLVKQT
                     TMSYPPANWKRMRRELDHAGVGQNYVVIQPTARQIFKCWDNAKFSAVIDALHARGYEV
                     VLTSGPDKDDLACVNEIAQGCQTPPVTALAGKVTFPELGALIDHAQLFIGVDSAPAHI
                     AAAVNTPLISLFGATDHIFWRPWSNNMIQFWAGDYREMPTRDQRDRNEMYLSVIPAAD
                     VIAAVDKLLPSSTTGTSL"
     gene            36821..37945
                     /locus_tag="NBI74_001697"
     CDS             36821..37945
                     /locus_tag="NBI74_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HCD1932055.1"
                     /translation="MIVAFCLYKYFPFGGLQRDFMRIAQTVAARGHHVRVYTQSWEGE
                     CPDVFELIKVPVKSHTNHGRNAEYFAWVQKHLREHPVDRVVGFNKMPGLDVYYAADVC
                     YAEKVAQEKGFFYRLTSRYRHYAAFERATFEQGKPTQLLMLTDKQIADFQKHYQTEAE
                     RFHILPPGIYPDRKYSQQPANSREIFRKKNGITEQQYLLLQVGSDFTRKGVDRSIEAL
                     ASLPDSLRHNTLLYVVGQDKPRKFEALAEKRGVRSNVHFFSGRNDVSELMAAADLLLH
                     PAYQEAAGIVLLEAITAGLPVLTTAVCGYAHYIVDANCGEAIAEPFRQETLNEILRKA
                     LTQSSLRQAWAENARHYADTQDLYSLPEKAADIITGGLDG"
     gene            37938..38735
                     /gene="rfaP"
                     /locus_tag="NBI74_001698"
     CDS             37938..38735
                     /gene="rfaP"
                     /locus_tag="NBI74_001698"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(I) kinase RfaP"
                     /protein_id="HCD1932056.1"
                     /translation="MVELKEPFATLWRGKDPFEEVKTLQGEVFRELETRRTLRFEMAG
                     KSYFLKWHRGTTLKEIIKNLLSLRMPVLGADREWNAIHRLRDVGVDTMYGVAFGEKGI
                     NPLTRTSFIITEDLTPTISLEDYCADWATNPPDVRVKRMLIKRVATMVRDMHAAGINH
                     RDCYICHFLLHLPFSGKEEELKISVIDLHRAQIRAKVPRRWRDKDLIGLYFSSMNIGL
                     TQRDIWRFMKVYFGMPLRKILSFERNLLNMASVKAEHIKERTQRKGL"
     gene            38772..>39037
                     /locus_tag="NBI74_001699"
     CDS             38772..>39037
                     /locus_tag="NBI74_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HCD1932057.1"
                     /translation="MTIYFINWVADYELKMIQYLKKKHKIKNITTPKKYNWVNKKISK
                     LGMDNAWLGRLFIKHHLNAVKKDDIIIFNDSVINKSINKQILKN"
BASE COUNT         9449 a         9433 c        10342 g         9813 t
ORIGIN      
        1 taacaaaaat cctccagcct tgtaggccag aggataaaat acttaattga ggaaaacatc
       61 agggaaaagt atttttgact taactttccc ggctggagag tgaatcaatg taatcagcgt
      121 accattgcat catttcacgg cggccatcga gatacagggc atgattatag gtacccctga
      181 tggaattttt atccacatga gctaactgca tttcaatcca ggcggaatta aagccttgtt
      241 catgcagaat tgtactcatg gtgtgtctga aaccgtgccc tgtgagtctt cccttatatc
      301 caagtaattc aattacttgg ttaatgcttt ccttactaat tggtttgcgg ggatcatttc
      361 taccgataaa gactagggga tgatgttttg agattggttc aagctgctta aatagcataa
      421 tgacctgctc agagagcggc acaatatgag gtcgcttcat cttcattact tctgccggga
      481 tctcccacaa ttttgtctca aaatcgatat cttcccagcg tgcaaaacgc agttcttgtg
      541 ttcgtacgcc ggttaacata atgatctgag tagctgtctt agtgattata cttccggtat
      601 aacccgccag atcgttgagg aagtggggaa gttcattagc agtaagaaag ggaaaatgta
      661 cttttttcgg tgtagctaaa gcactggcaa gatcgggggc tgggttatag tctgcccggc
      721 cagtaacaat tgcatagcgg aacacctcac cacagcgctg gcggaccttg cgcattttct
      781 ccagagctcc acgtttttcc attttacgca gtgcttccag taactccatt ggtttgattt
      841 cggcaatagg gcgcttgcca atgtagggaa aaatgtcttt ttcaaaggta tcaatgattt
      901 catcacgata gcgtaatgac caccgatcgg cttttgactg gtgccattcg cgagcaatag
      961 cctcgaacgt attttgcaga ctcatttgct gcgcgatttt ttcttccttt tttgcctccc
     1021 ctggatcgcc tccagcagcg aggagtttcc tggcttcact gcgtttctcg cgagcatcgg
     1081 ctagcgttac atcggggtat acgccaatag aaagcatctt ttctttacca gcaaagcggt
     1141 acttcatgcg ccagtattta gaaccattag tgttaatcag tagatacata ccgccgccat
     1201 cagcaagctt atagggtttg tctttgggtt ttgcggtttt cactttgata tcggttaatg
     1261 ccatgacatc ctccggcttt tgatgaattc atgttcctga gggggatttc tgccagtaac
     1321 taacctgata cccccaatac accccttgta taacaaagat gttgttaaaa gccgttgtta
     1381 tatatcatat ttttcctatt atgggggtgc tggtatttca ataggaaata acacccccta
     1441 aagtaccccc aaaagaaact tgatacgggg tgaagtaggt tgacctctgg atacaaataa
     1501 atactgatat acaagggatt agaagagatt tattgacatt gggagagttc agaagaggta
     1561 aactggcgga agatcacagg agtcgaacct gcccgggacc gctggcggcc ccaactggat
     1621 ttgaagtcca gccgcctcac cggagacgac gatcttccgc gcctcgattg ctacatggag
     1681 gcggggcgca ttatagctac ttccttgagt ttctacatcc cccagatcag ttttttccgc
     1741 ctaatacacg cagcgtatta tcacgaaagc attatagttc atataaaact catattttac
     1801 aatgatatat gagcgaaatt aacgctacca cgatcacata aaacaagaat cataaattaa
     1861 taaccagata tcggaatatt cgctctcgca gggatggttt acaaaatgcg ttatcggtct
     1921 cagcacccct atagcatcaa ggaaaagcag atgaagagtg aagtgttgtc cgttaaagag
     1981 aaaattggtt atggcatggg agacgccgcc agccacatta ttttcgataa cgtaatgtta
     2041 tatatgatgt tcttttatac cgatattttt ggcattcctg ccggatttgt cggaaccatg
     2101 tttttggtcg ctcgtgcact ggatgcgatt tccgatcctt gcatgggatt gttggccgat
     2161 cgaacgcgct ctcgctgggg taaatttcgg ccgtgggtac tgtttggtgc actgccattc
     2221 gggatcgtct gtgtactagc ctatagcacg ccagatctca gtatgaacgg aaaaatgatt
     2281 tatgcagcaa ttacttacac cctacttacc ttactttata ccatcgtcaa tatcccttac
     2341 tgcgcattgg gtggtgtaat caccaatgac ccgactcagc gtatctcgct gcaatcctgg
     2401 cgttttgtgc tggcgaccgc gggaggcatg ctctctactg ttctgatgat gccactggtt
     2461 aatttaattg gtggtgataa taaacctctc ggtttccagg gcggtatcgc ggtcctttac
     2521 gtggtggcat ttatgatgct ggcattctgt ttcttcacca ctaaagaacg cgttgaagca
     2581 ccacctacaa cgacgtctat gcgggatgat ttacgtgata tctggcaaaa cgaccagtgg
     2641 cgtattgtcg gtttcctgac cattttcaat atcctggcgg tgtgcgtacg cggtggggcg
     2701 atgatgtatt acgtcacatg gattttgggt acgccggaag tatttgttgc ttttctcacc
     2761 acttattgcg tgggtaacct gattggttcc gcactggcaa aaccgctgac cgactggaaa
     2821 tgtaaagtca ctatcttctg gtggacgaac gccctgctgg cagtgattag cctcgcgatg
     2881 ttctttgttc ctatgcaggc cagcatcacc atgtttgtct tcatcttcgt gattggtgtg
     2941 ttgcatcaac tggtgacacc tatccagtgg gtaatgatgt ccgataccgt cgactacggc
     3001 gagtggtgta atggtaaacg cctgaccggg atcagttttg ctggcacgct gtttgtgctc
     3061 aaactggggt tggccttcgg tggtgccctt atcggctgga tgctggctta tggcggatat
     3121 gatgcggcag aaaaagcgca gaacagcgcc acaattagca tcattatcgc gctattcacg
     3181 attgttccgg cgatctgtta cttgctgagc gcgattatcg ctaaacgcta ctactcgctc
     3241 acgacgcaca atctgaaaac cgttatggaa cagctggcgc agggcaaacg ccgttgccag
     3301 caacaattca cctctcaaga agtgcagaac taaggaacgg caatgaaaat tagcgacgga
     3361 aactggttga ttcaacctgg cctcaatttg attcacccgc ttcaggtgtt cgaggttgaa
     3421 cagcagggta atgaaatggt ggtctatgct gcccctcgtg atgtgcgtga acgtacctgg
     3481 cagcttgata cgcctttatt tacgctgcgc tttttctccc cacaggaagg tattgtcggt
     3541 gtacggattg agcattttca gggggcgctg aataacggtc ctcattaccc gctcaatatt
     3601 ttgcaggacg tgaaggtcac tatcgaaaac acagaacgtt acgctgagtt taaaagtggc
     3661 aacttaagtg cgcgtgtcag caaaggtgag ttctggtcac tggattttct gcgcaacggc
     3721 gaacgtatta ccggtagtca ggtgaaaaat aatggctacg tgcaggacac gaataatcaa
     3781 cgaaattata tgtttgaacg gctggatctt ggcgttggcg aaacagttta cggcctggga
     3841 gagcgcttta ctgccctggt gcgcaatggt cagacggtag aaacctggaa ccgggacggc
     3901 ggcacaagta ctgaacaggc gtataaaaat atcccgtttt atgtgactaa ccgtggttat
     3961 ggggtactgg tcaatcatcc ccaatgtgtc tcttttgaag tgggatcgga gaaagtctcc
     4021 aaagtacagt tcagcgttga gagtgaatat ctcgaatact ttgttatcga cggcccgacg
     4081 ccgaaagcgg tacttgatcg ttatacccgt tttactggcc gtccggcgct gccgcccgcg
     4141 tggtccttcg gtctgtggct aaccacttca tttaccacca actacgacga agcgacggta
     4201 aacagcttta tcgatggtat ggcggaacgc aatctgccgc tgcatgtttt ccactttgac
     4261 tgtttctgga tgaaagcctt ccagtggtgc gattttgagt gggatccgct gacttttccg
     4321 gacccggaag ggatgatccg ccgcctgaaa gcgaaagggc tgaaaatctg cgtctggatt
     4381 aacccctata ttgggcaaaa atcccccgtc tttaaagagt tacaagagaa aggttattta
     4441 ctcaaacgcc cggacggttc gctgtggcag tgggataaat ggcagccagg tctggcgatt
     4501 tatgacttta ccaatccgga tgcttgcaaa tggtacgccg acaaactgaa aggtctggtc
     4561 gcgatgggcg ttgattgctt taagaccgac tttggcgaac gtattccaac cgatgttcaa
     4621 tggtttgatg gttccgatcc gcagaaaatg cataaccatt atgcatacat ctacaacgaa
     4681 ctggtgtgga acgtgctcaa ggacaccgtt ggtgaggaag aagccgtctt gtttgcccgc
     4741 tcggcctccg ttggtgcgca gaaatttccg gtacactggg gtggcgactg ttacgctaac
     4801 tacgaatcaa tggcggaaag cctgcgcggt ggtttgtcta ttggcctttc aggttttggc
     4861 ttctggagcc acgatatcgg cggctttgaa aataccgctc cggcgcacgt ttacaaacgc
     4921 tggtgcgcgt ttggtttgct ttccagccat agccgtttac atggcagcaa atcttatcgt
     4981 gtgccgtggg cctacgatgg tgagtcctgt gatgtggtgc gcttcttcac gcaactgaaa
     5041 tgccgcatga tgccgtatct gtatcgtgaa gctgcacgtg caaacgcccg gggtacgccg
     5101 atgatgcggg ccatgatgat ggagttcccg gacgatccgg cttgtgatta ccttgaccgt
     5161 caatacatgt taggcgacaa cgtgatggtt gcgcctgtgt tcactgaaac gggcgatgtg
     5221 cagttctacc tgccggaagg tcgctggaca cacctgtggc acaacgatga actcgacggt
     5281 agtcgctggc ataaacagca gcacagcttc ctgagtctgc ccgtttatgt gcgtgatagc
     5341 accctactgg cgctgggcaa caacgatcaa cgtcccgatt acgcgtggca cgaaggtacg
     5401 gcattccacc tctttaatct gcaagacggg catgaagccg tctgtgaagt gcccgatgct
     5461 gacggttcgg tgatctttac tttaaaagca gcacgtactg gcaacacgat tactgtgact
     5521 ggtacgggcg aggcgaagaa ctggacactg tgcttgcgca atattgtgaa agtaaatggt
     5581 ctgcaaggcg gttcgcaggc tgaaagtgag cagggactgg tggtgactcc tcaagggaat
     5641 gcgctgacaa ttacgttgta atctattgaa gaatgccgga tacgaatggt tttgtgtcgt
     5701 atccggtaaa taatctcaca tctggtaata aatatttctt ccatcgtatt tgcgagcatg
     5761 atcacgatta ttcatcacga aaatgtgatc tgtgctgaaa aatatccctt aaccgccttg
     5821 ctttgtttta tcactgtccg gattacggac agattttagt attgttgtga aattattctg
     5881 ctttatattt accctcagag atggcaactg aagctcttcc atatggggct tcttaatctg
     5941 tgataagggt aaaaaatgca aatgattacc tcgcgacaaa acagattatt acgattcctt
     6001 ttacctcgaa gggaatatac gactattgtt acgattgccg gctatttaaa tgtttcggaa
     6061 aaaaccattc aacgtgattt acgtttactt gagcaatggc tggggcaatg gagaataagt
     6121 gttgagaagc gtgctggcgc gggtgtgatg ttaagcgcgg agaatattgc tgatttgctg
     6181 catcttgatc atttgttggg agcagaatgt gaagatgttg atggtgtaat gaataacgcc
     6241 aggcgcgtta aaatagcgtc gcagttatta agtgaaacac cgaatgaaac gtcgatcagt
     6301 aaattgtcag aacgctactt tatcagcgga gcctctattg ttaatgacct gagagtaatt
     6361 gagtcctggc ttgcgccgtt ggggttatcg ttgatccgca gcccaagtgg tacgcatatt
     6421 gaaggtagtg aagggcaagt ccgacaggca atggcattac tgattaacgg cattattaac
     6481 cataatgagc cgcaaggtgt cgtgtattca cgtctggatc ccggaagcta taaagcatta
     6541 gtccattatt ttggagaaga agaggtgtta tttgtccagt cattgttact ggatatggaa
     6601 aatgaattaa gttggtcttt gggagaacct tattacgtta acatttttac tcacatcctt
     6661 attatgatgt atcgcaacac gcacgggaat gcgttatcaa gagaagaaga tcaaaccagg
     6721 caatatgatg aaaatatctt taatgttgcc agtcagatga ttcataagat agaacaacga
     6781 attgcacata cattgcccga tgatgaagtc tggtttattt atcaatatat catttcatca
     6841 ggtgtggcga ttgatggaca aaaagatgtt agcattattt cacatatgca ggccagcaat
     6901 gaagcgcgtc tgattacctg gcgtttaatt acggtattca gtgacatcgt ggactgcgat
     6961 tttagtgaag acagcgcatt atatgatggc ttattggtgc atattaaacc gctgattaac
     7021 cgactaaatt atcgtattca tatccgtaat ccattgctgg aagatattaa agcagaatta
     7081 gcggatgtct ggcggttgac gcaatatgtg gtgaatcagg tatttaaaac ctggggtaag
     7141 aatgcggtga gcgaggatga agtgggttac ctgaccgttc attttcaggc tgcgatggaa
     7201 cggcaaattg cccgtaaacg cgtattactg gtctgttcaa ccggaatagg aacttcgcat
     7261 ctactgaaaa gccgtattct gcgagcattt cctgaatgga cgattgttga tgttatttca
     7321 gcagcgaatt tatcacaggt tttgcctgac aatatcgaac tgattatttc gactattaat
     7381 ttgcctacag tcactatgcc ggtcgcttat gttaccgctt tttttaatga tgccgatatt
     7441 aagcgggtca ctgaaatggt gattacggaa aaattacatc atgcgacgtc tcgggtcgtt
     7501 gaaatttaat gtcagaattt tatgaggtat gacgaatgga cataacaaaa atcctgaata
     7561 cgaatcgcgt tattttagat atgcaagcga cgaataaagc ggaggcgatt gaagaattaa
     7621 ctaatcttct caaaaaagat ggtgcgatcg attgtcggga aacctttatt aaagatgtct
     7681 ggcagcgcga agcggaaggc tcgactggtt ttgaaaatca tattgcgatc ccgcatggta
     7741 aatcttctgc ggtaattaat accacgctgg caattggccg tacccgccag gatattccct
     7801 gggaaacgct ggatggcagt agcgtgcgct gtattatttt atttgcggta cgtcttgaag
     7861 atcaaaacac cactcatatc cgtttacttt cccaggtcgc gggcgcgttg gcggatgatg
     7921 acatcataga acaattgtta gttgagacga gtccgcaaaa aatcattgat ctctttagtc
     7981 agtatgcaca atccgatgtc tgctaaataa cagggagtat gtcatgaata ttgtctgtgt
     8041 tgccgcctgt acggcgggta ttgctcatac ctatattgct cgtgaaaagt taatcaaagg
     8101 agcaaaagct ctgggccata ccattaaagt tgaaacccag ggaaccattg gtacagaaaa
     8161 tgaattacag gcggcagata tctctgctgc tgacgtcgtg attcttgctg tagatgtgaa
     8221 aataaaagga gaagaacgct tcaccaataa acgtattgtg cgagtaaaga cagagatcgt
     8281 cattaaatcg cccgttcaat ttcttgaaaa ggtcgaaaaa tcactgggaa acaaatgatc
     8341 cttatactgg agggatctta atatgaaaga caataaaatt ccggtttcac aggaaattaa
     8401 aaaacattta ttaaccggca tctcgtggat gatacccctt attgtggccg ccgggatatg
     8461 tattgcgttg gggcaagttc ttggcggtac agatgttggt gaaaaaacag gaacaatccc
     8521 gtggatgctt aatcagattg gcggctgggg aatggggctg attgtgccgc tgattagcgc
     8581 ggcaattgcc tattctattg ccgatcgtcc cggatttgcc ccgggcctga ttgtcggctt
     8641 tctctgcggg caaatccata ccggatttat tggcggtatg ttgggtggtt tcctggtggg
     8701 ctacaccatt ttgctactta aacgctatat ccgcctgccg cagtcgatgc aaggactcat
     8761 gccaatcatg gtgttgccgg tattaagcac cattatcggt gggctgttaa tgatgacgct
     8821 gattggtaaa cccatcgcct ggttacaaga agcactgatt catttgctcg agtcgatgca
     8881 gggcggatcg cgcttcctga tgggggccat acttggcgcg atggcaacct tcgatttcgg
     8941 tggtccggtc aacaaaacca tgtcgctgtt ttccgatggc ttgctggtca gtggcgtcta
     9001 cggaccagaa gccgtgaaat ttgtcggctc aatcatccct ccgtttggca tcacactttc
     9061 gttcttactg acacgccata aatacacccg tgcggagcgc gaggcgctga aagccgcgtt
     9121 tccgatgggg atctgcatga tcactgaagg agtcattccg atcgccgcgc gtgacctact
     9181 gcgagtagtg ggatcttgtg tggtggcatc ggcggtcgcg ggcggcttaa tcatggtgtg
     9241 gggcgtggag agtccggttc cgcatggcgg catgtttgtt gtgcctctgt tcacccatcc
     9301 tctgctgttt tgcctggcgc tgggtatagg cacggtgatt tgtggcgtga tgctttcact
     9361 ctggaagaaa ccggtaacgg aacgcgacga agagtttgac gaactgagcg atcaaaagct
     9421 gaaagatgaa gagatcactt tcaccctcga ataaccggag gccctatgtt tgccgatatg
     9481 aaaagtatgg tgatgaaagc ctggcatgaa cattacgcct tgctggcgat caactgtatg
     9541 aatctggaaa gtgctcacgc agctatccgc gtggcggaga agaatcgcgc tccgatcatt
     9601 ctcaatctct atcaggggca tctggcgcat tttcctgcac ccgtcgcggc ggcagtggtt
     9661 aaaacgctcg cggaatctgc ctcagtaccg gtcgctctgg cgcttgatca tggcaaaaat
     9721 ccggactgta ttcgtcaggc gtttcgtgct ggtttcagcg gattaatgat tgatgcttct
     9781 gcttttcccc tggaggaaaa cgtgcgccag accaaggcgg tggtggaact cgccgccagc
     9841 gcgcagctct gcgtggaagg cgaactgggc catctggcag atgcgccgcg ttacgaccag
     9901 gccgccaatg cagacctgat gacgcaaccc actgatgtgg aaccgtttat cgcgcagaca
     9961 ggcattgatc tactggccgt ctcggtaggc acggcgcacg gcatgtacgc gcctggcgtt
    10021 gtacctgcta ttgattttca gcgtctggcg gagatttcgc gctgctcgag cgtgcctctg
    10081 gcattacatg gcggtagcgg tacgccgttc gatcagctcc agctctgtac gacgctgggg
    10141 gtagcaaaaa tcaacgtggg ggcggccatt tttgaacggg ggaaatccgc gctcctgcat
    10201 accctctgtc acgatatatc cattgaactg gtggacgcgc tgaaagctat ggaactggcc
    10261 ttcgaagaag cgattacacc gtatttacag gcgagcggct ctatcggcaa agcctgattc
    10321 attttctgtc atggccttta tgccagtgca ataacgcacc ggcagggcgg actacacgct
    10381 gaaactggag atttaacatg ttagtttcga tgaaagaatt actgcaacct acccgccagc
    10441 acggctttgc cattggcgca tttaatgtgg cggataactg tttccttcgc gcagtcgtgg
    10501 atgaagcaga agcgacaaat actccggcga ttattgccat ccatcccagc gaacatgatt
    10561 ttgtgggcga tgcctttttc gctcacgttc gcgaaattac ccagcgtagt ccggtcccgt
    10621 ttgtactgca cctcgatcat ggtgcctctg tagagcatgt tttgcgtgcg attcgctgcg
    10681 gatttacctc agtaatgatc gacggttcgc tgctgccgta tgaagaaaac gtggcgctga
    10741 ccgcagaagt ggttcgcctc gctcacgcgg ttggcgtgtc ggttgagggt gaactgggaa
    10801 ccatcggaca aacaggaacg tcggtagaag gtggtgtttc acaagtgacc tacacagatc
    10861 cggcgcaggc cgccgatttt attgctcgta caggtgccga cacactggct gtggcgattg
    10921 gtacagcgca tggcatctat ccaaaaggga tgcagccgaa gctgcaaatg gacattttgc
    10981 gcgacattgc cggacgtatt gatattccgc tggtgctaca cggtggttcg gcaaacccgg
    11041 acgcggagat tgctgaatca gttacgttcg gcgtcggcaa aatcaatatc tccagcgata
    11101 tgaaatacgc ctatttccag aaagtgcgtg aaattctcgc taaagagagc tggtgggacc
    11161 cgaacgtcat ctatccggat gcgattaacg cggcgcggga agtcattcgc cacaaaatga
    11221 aactgttcgg ttcgctcggt aaagcatcac tgtactaagc cagaagggcg gggtgacccg
    11281 ccttttacgc aacaaggaac gagatatgca ctatctgggg ttggatattg gtgggaccaa
    11341 aatcgccgcc gttgtcatgg atgcgcatgg ctgggagatt cgccgttacc gctgcccgac
    11401 gcaaaagtcg acatatcaac aatttgtctc atgcgttgtg gcgcttatcg agcagattag
    11461 gcgggacgtt caacgaccga tgctgacagg gatcgcctta cccggcagta tctcgccact
    11521 cactggcctg attaaaaacg cgaatattca ggtgattaac ggtcatgcgt tacaggctga
    11581 tttgcagcaa ttgcttgggc aaccggtggt gatagccaat gatggtaact gtttcgcgct
    11641 atcagaagct tgcgacggtg ccgggcaaga ttatgacgtg gtatttggta ttacgcttgg
    11701 tacgggctgc ggcggcggca ttgccatcaa gcaacgaccg tttatagggg cctggggaaa
    11761 tgctgccgaa tgcggtcata tcacgttgcc aggctatacg gagcaggaag atggtcaatc
    11821 agtcagttgc tattgcggca aacacaactg cgtggagtcg tttgtttccg gcagcggttt
    11881 tagtgaacgc tatcaacaga tgactggtaa cttgctcact cctgcggcga ttgtcaccct
    11941 ggcacaacgt ggtgatgctt gtgccatgca gcaggtggca cgttttcgcc aacagcttgc
    12001 ccgcacgctg gcaaccatcg ttaacgttgt tgaccctggc gtgattgtga tcggcggcgg
    12061 gctttcgaat gtggaactgc ttatcaccga tctgaacgca gaagtcgctc cactggtttt
    12121 caccgatcaa ttcaccactc ccattgtaaa agcacagcac ggcgacagta gcggaatgcg
    12181 tggcgctgcc tggcttgcta tgcgcaacgg agaagccaat gaaacgttca caaattaatt
    12241 atgttattga taaagcacat gccattgcgc agaccttccg cgtttgtttg ccagagttcg
    12301 cctattttac ggtggatgac tggctgcagc gggaacggga taactggcaa gaagtggtcg
    12361 acttacagct cggttgggat atcaccgatt ttggtcgtgg tgatttcaac caaacgggac
    12421 taacactgct aacgatgcgc aacggcgcgc tggggtcggc ggcatatcca aaaccgtatg
    12481 ctgagaaaat gttgcagatc caacaagatc agcagactcc ctggcatttt cacacccaca
    12541 aaatggaaga tatcctcaat cggggaggtg gagacttatg catgcagttg gcctgggcca
    12601 acgaagccaa tctctgtgat gatcaacgag tcattaccgt gagtgttgat ggtcagcggc
    12661 gtaccatgaa acctggtgaa acgctggtgc taaagccggg gcagggcatt tgtctgccgc
    12721 cgcgtctgta tcatcgcttc tgggcggaaa aagcgtttgt tctcggctgg gaaatttcga
    12781 tggtaaatga tgaccaacac gataactatt ttcttgagcc gggaggacgt tttccggcaa
    12841 tagaagaaga tgaaccggtg aaatggttat tgtgcagtga gtatggaatt ttgcgctaat
    12901 aacggggaac gctagcgctg atatccgcta gcgtccttat ttttactgca ccggctccgt
    12961 cgacgtaact tcaccactgg aaactacgcc cgcattcatg gtttgagtca cttgctgctt
    13021 tgcggcattc acggcatgca attgcccgct aaccgtaggt ttcagcggga cattgacctg
    13081 aatatcgcca ctggcggtaa gctgaatatt gccgtcgcca gtcaacggta attcaggcca
    13141 tccccaatgt tgcaaaatat tcaccggcac accgcgtcca ttcaggctga tatgggtctg
    13201 acgttgtggc gtttgtgaaa cactggcggt ggcttccaga atgccttttt cagtaaatgc
    13261 actcagttcg ctgatattca ccgtgctgct gttggcagtt agcgccagcg acggacgacg
    13321 gacatcaaca cgattaaacg ttgcggcggc ggcattcaga ttcgccgagc cactccagac
    13381 gccccattta tgatcggtaa ccagtgtcag attggcaccg taaccatcga gcgcggtgag
    13441 ctgccacggg aagtcagggg cgatatcaat gatcagattg cggctggcgc taaatctctt
    13501 cagttgcagg ctgtttaacc aaccgggtgt cgtttccatc cacaactgtt gccagttttt
    13561 tggcaaggta tattctagtc cggcaattgc cgcatcatca aggatcaaca ttttcccgtc
    13621 acgcaaccag ttccctgacg ttctgaccat gcccccttcc cagcggctgg tgaactggcg
    13681 cagtgctacg ccctgcgggg aaaattccgc gtttataatc ggatcaaata aatgcagcga
    13741 gccataaata aactcgctgg cgttcatcga cagtttgcct tcctgtgtct gccagtcatc
    13801 tttactgaag gtcatgttgc gcaagctgag atcgaggtcg gtcaccgccc agtccggacc
    13861 ttgcaaacga gcgtcgatga cttccaggcg accaatctgc aacgacggga cagagcgtaa
    13921 tggtgcaaag aagtcggtta gcgatttttc gctttgtaga cggatatccg ccaggcgcag
    13981 gttttccact tgccagctgc cgtcagcgtt acgctgcgcg tttccggtta atgtcccgcg
    14041 ggcgatgtcg gcacccaggt tagtcagcgt aacgcgatcg ttatcaatac tgccttcaat
    14101 cagtacattg gtggcaggaa catcattgag cgaaagcgat ccggcactaa actgaatctg
    14161 cgccttcgta cccagcactt taccggcttc tggtgaccac ggaaccacgc cgccatttac
    14221 ccgctgcgcg ctcagtttcc attcgctatt cgggctatta aacgccatat cacgcagttg
    14281 cagacgatcg gctttgaacg gtagcggcgc ggtctggtcg gtgagattca gcgtcccgtt
    14341 ttccagcagg atggtatcga catggcgtgg ttcggtcagt tgccgactgc ttagcgcaat
    14401 atcgacactt tttgccacca gggtcgcggg ctgaccatca cggccaaacg tgacgttctc
    14461 cagcacgata tgagatggcg cggaaaaacg gtgatccatc gccccgaagg ccagatgata
    14521 gtcgctattc tcggaaaccc atgcgctgat atgttctgct ccccagcggg tttgcagaag
    14581 aaaatagagg ccagcgatca ccactaacag agcgatgaaa atgtagagaa gcagcttccc
    14641 aataaatttc atggtcttcc atcccgtgaa atgcacataa gggagttatg cacgatttac
    14701 gcgcaatcct caaggcggga atggtgaaag aggtgagact gcggcggtaa ttatcttacc
    14761 gccgccagtg aattactgtt tttctggtgg gaaaatcaga ttcagcacga tggcagtaat
    14821 accgcccgcg gcaatcccgg aggagagtag gtttttcagc cattcagggg caaactgcaa
    14881 aatcagcggc tgctgagaca cgcccagacc gaccgccagc gacagcgcga taatcagaat
    14941 cgcccggcgg ttcagcggct cacgagaaac gatacgcaca ccggaggcgg cgatggtgcc
    15001 aaacattaca agcgttgcgc cgcccagaac cggttctgga atgtgttgta caaaaccgct
    15061 cactgccggg aacagaccca gcacgatcaa catcagcgcg acgacaaaac cgacatagcg
    15121 gctggcaaca ccggttaact ggatcacccc gttgttctgc ccgaagcagg agttcgggaa
    15181 ggtattaaat accgccgaga caaacgagtt caggccgttt gccagtacgc cgcctttcag
    15241 gcgtttcatg tacagcggac cggacactgg ctgttcggaa acgtcagagg tcgccgtgat
    15301 atcgccaatg gtttccagcg aagtgatcat aaagaccagc atcagcggca gcagcagact
    15361 ccattcaatg ccaagaccgt aatagagcgg cgttggcacc ataatcagtt cttgcgtcat
    15421 cggttcgttg ctttctggca acatgcccat aaaccacgcc agcgcatatc cggccgccat
    15481 cgcaatcacc agtgaggcca cgcgtaagta agggttacgt tgacggttaa gcaggataat
    15541 taaggctaag accacgcctg ccagcagcag atttttcggt gcgccgaagg tgttatcgct
    15601 catggctgcg taaccgccgc caatggaggt cagtccaacc tgaattagcg acaggccgat
    15661 aatcatcacc acaacgccag aaaccagcgg cgtaataatg cggcgcgcca gatgcagaac
    15721 gcgggagatc accatctcgg tacaacttgc cagcatcaac gtgccgaaca aagctgccat
    15781 catggtagga acatcagcac caccggtctt cagcgctgtg ccgcccataa tcagcggagc
    15841 aacaaagttg aagctggtgc cctgaataga caacaacccg gagccaaccg gaccccaggc
    15901 cttaatttga ataatcgatg ccacaccgga ggcaaacagc gacatactaa taatgtgttg
    15961 cgtgtcttgt gccggtaaac ccagcgcctg gcagattaat agcgctggcg tgatcaccgc
    16021 aacgaacatc gccagcaaat gctgacaggc ggcaaacagg gtttgaggaa gcggcggacg
    16081 atcttcaaga cggtaaatca gttcgctgtt ttgagtctgc acaaccggtt gcgcattttc
    16141 tgactcgagg gtggaaacag acatcgaaaa caatcccgtg gtagcaaagc gggcatttta
    16201 gctgaccgaa agagaaaagc aaacgtttgc cacatcctca tctaaatatt taaatgagat
    16261 aaatgtactt ttttatataa cttttagttg ttttctgctt aaaatccatg ccatttttct
    16321 agtttcagcg aagaattcga accaggaaag catgactcga tactcgtcat atttcgcctt
    16381 actgcaaatg cgcgatgaag tatgacgagt atgaaagagt gatgcggaca caaaggagta
    16441 actatgtttc atctcgatac tttagcaacg cttgttgccg caacgctgac gttgctgctc
    16501 gggcgcaagt tggtccattc cgtctccttt ttgaagaaat acaccatacc ggaacctgtt
    16561 gcgggtggtt tattagtggc gctggcgctg ctgatactga aaaaaagcat gggctgggaa
    16621 gtcaactttg atatgtccct gcgcgatccg ttgatgctgg ctttcttcgc caccattggc
    16681 ctgaacgcca accttgccag tctgcgagct ggtgggcgtg tggtgggggt tttcctgatt
    16741 gtggttgttg gcctgttggt gatgcaaaat gccattggca ttggtatggc tagcctgtta
    16801 gggcttgatc cgctgatggg gctgctggcc ggttctatta cgctttccgg cggtcacggt
    16861 acgggtgctg catggagtaa attgttcatt gaacgttatg gtttcaccaa cgcgacagaa
    16921 gtggcgatgg cctgtgcaac gttcggtttg gtgctgggcg gcttgattgg cggtccggtg
    16981 gcgcgctatc tggtgaaaca ctccaccaca ccgaacggta ttccggatga ccaggaagtt
    17041 ccgacggcgt tcgaaaagcc ggatgtgggc cgcatgatca cctcgctggt gctgattgaa
    17101 actatcgcgc tgattgctat ctgcctgacg gtggggaaaa ttgttgcgca acttttggct
    17161 ggtactgctt ttgaactacc gaccttcgtc tgtgtactat ttgttggcgt gattctgagc
    17221 aacggtctgt caatgatggg cttttaccgc gtctttgagc gtgcggtatc cgtgctgggt
    17281 aacgtaagcc tgtcgttgtt cctggcgatg gcgctgatgg ggctgaaact gtgggagctg
    17341 gcttcgctgg cgttgccgat gctggcgatt ctggtggtac agaccatctt catggcgttg
    17401 tatgccatct tcgttacctg gcgcatgatg ggcaaaaact acgatgcggc agtgctggct
    17461 gcgggtcact gtggttttgg cctcggtgca acgccaacag caatcgccaa catgcaggcg
    17521 atcactgaac gctttggccc gtcgcacatg gcgttcctgg tcgtgccgat ggtcggtgcg
    17581 ttctttatcg atatcgtcaa tgcgctggtg attaagctgt atttgatgtt gccgattttt
    17641 gccggttaac cgatgaagcg gcggtcttct caccgccgct ttttacgcat tcgagtaacg
    17701 ttctgtttct ggcatccagc gttctatcag ggcttttgcc tgttgtgggt aacgttcgtg
    17761 aatatgtcgc gccaggcgct gaacttccgg gatcatcgcc tgatcgcgca gtaaatccgc
    17821 cactttaaat tcagcattac ccgtctgacg tgtacctaac aattcgccag ggccgcgaat
    17881 ctccagatct ttctgtgcaa tcacaaaacc atcgttgctg tcgcgtagta cctgcaggcg
    17941 gatttgcgcg gttttggaga gcggcgtttt gtagagcagc acgcagtgag aagccaccgc
    18001 gccacgacct acacgcccgc gaagctggtg taactgcgcc agacccagac gctccgggtt
    18061 ttcgataatc atcagactgg cattgggtac atcaacaccg acttcaatga cagtagtggc
    18121 aaccagcagg tgtagctcgc cttgtttaaa cgatgtcatc accgcctgtt tctcggcggg
    18181 tttcatccgt ccgtgtacca ggccaacatt caactctggt agcgccagtt tcaactcttc
    18241 ccaggtcgct tccgccgcct gcgcttccag taattccgac tcttcaatca acgtacaaac
    18301 ccagtacgcc tgacgccctt cagtcataca ggcgtggcgc acgcggtcaa tgatgtcggt
    18361 acggcgggta tcaggaatag cgaccgtagt cactggcgtg cggcctggtg gcagctcatc
    18421 tatcaccgag gtatcgagat cggcatatgc agtcatcgcc agcgtgcggg ggatgggcgt
    18481 ggcggtcatg atcaactgat gcggatggaa gccctgctgc tggcctttct cccataacgc
    18541 cagacgctga tgcacgccaa aacgatgctg ttcgtcgata atcaccagcg ccaggccgtt
    18601 aaactgcacc tgttcctgga aaatggcgtg cgtaccaaca atcatctgca cctgaccgct
    18661 ggcgatggct tcttgctgag ccagtcgtgc tttacctttc tgttttccgg cgagccagcc
    18721 tacttcgata ccgagcggtg caaaccagtt acggaagtta ttggcgtgct gctcggcgag
    18781 taattcggtt ggtgccatca gtgccacctg tttgccgtgg gcaaccgcac gcaacgcggc
    18841 gagggcggcg accagcgttt taccggaacc tacatcgccc tgcaccagac gcatcatcgg
    18901 cacatccagc gccatatcgc gctcgatctc cgccactacg cgtgcctgtg cgcccgttgg
    18961 cttgaacggt aaggcggcga ggagtttatt tttcagcgcg tcattggcgc tcagcggctg
    19021 agcatgaaaa cgttgtgcac cggcacgtaa cgccaacatg ctgaggttgt gcgccagcaa
    19081 ctcttccaga atcagccggc gttgtgccgg atgctgcccg gtttcgagat cgctaagctg
    19141 tagcgtcggc ggcgggcggt gcaaagtgcg caacgcttcc ggtagcgtca tcattccttg
    19201 tgacagttcc ggcggcagga gttcttcaat ggcgcatgtg tcgagcagat ccagcgcctg
    19261 gtcggttaat ttacgcagcg tagcctgctt tacgccttcc gtagttggat aaaccggtgt
    19321 gagcgtttcc tgtaactctg gcgtgctgag atcgccctgt acgcggtatt ccgggtggat
    19381 catctccgca ccatatttgc cgcgctttgc ttcgccatag gccagcacgc ggcggcctgt
    19441 cgccaggcta tttttcattg ccgcattgaa gttgaaaaag cgcatagtga gaatgccgga
    19501 accgtcgctg atctggcagg tcatcatccg ccgaccgccg aaggagatat tgcagttcag
    19561 cacttcgcct tccaccgtgg cataaacccc cggcagtagt tcaccgatgg ggtacagatg
    19621 tgtgcgatct tcgtagcgca gaggaaggtg taagagtaaa tcctgtacgg tatgcaggtt
    19681 gattttcgcc agcttgttac taagcgctgc gccaacgccc gttagggaac tgagcgggac
    19741 agcatctaac aggcgacctt tcatcgcgct taccctgcgg cctgcatggt ggaccaccag
    19801 tcagcatcgg cttcaatctc gccttgctga ttaacgtggg ggtagggcag acctttgcgt
    19861 tttgcgactt tcgccagcac cggatagccg ccttcaaaca gcaggcgttg ttgctcggct
    19921 tccggcaaca tgctgttttc acgcaggtac atgcccgcat tttgccgctg gcgctgggct
    19981 tcgtaaagaa tgagggctga ggcaacggaa acattcagcg actgcaccat gccgatcatc
    20041 ggaatgatga tatcctgatc cgccagggcc aacgcttcct gcgtgatgcc cgttttctcc
    20101 tgtcccatca aaatacaggt cgggcgagtg taatcaattt cgcggaaatc aacagcgtta
    20161 tcagaaagat gggttgccag aatctgcatg ccctggcctt tcagatgagc gacggcatcg
    20221 ccaatggtgc ggtgcgtttt cacctgtacc cagctgttac tacccgcagc tgccgaagcc
    20281 atggtgcgca tccggctgcc aggccagaca gcatgaactt catgtacgcc aacggcatct
    20341 gcggtacgaa taatcgcaga aacgttatga ggtttgtgga cctgctccat gcagacggtc
    20401 agatcaggct gccgcctggc gagcatttcg cagatgcgtg cataacgtgt tgggttcata
    20461 aaacattaat ttcggtttcg ggtgacttta atcacgtctg gcatcacgcg gattttgcgc
    20521 atgatattcg ccagatgcac acggtcacgg gcggtcagac gaataaaggc gctatagacg
    20581 cgaccatctt tctcttccgt attcaaactt tgaatattcg aggtcgtggt gttaattgcc
    20641 gccgtcaggt ttgccagcgc gccctgatga ttgaacatct ccaccttgat ttcggtgatg
    20701 aattcctgcg ccgtctcttt atcccattca acagccataa acttctctgg ctctttctgg
    20761 tagccacgga tgttacggca ggattcatgg tggatcacca gacctttacc ggggctgacg
    20821 tgggcgataa tcgggtcgcc aggaattggg cggcagcatt tcgcaaaggt gatcagcacg
    20881 ccatcggcac ctttaatggg cagatgtccg tgactttggg ttgccggtgg aatggaggcg
    20941 tccccatgtt gcagattttt cgcgaccacc acgctcattg cgttaccaag gccgatttca
    21001 gccagcagat cgtcaagcgt agccagcttc atgcggtcca gctcgcgctg aatattttcc
    21061 tgcgggattt cattgagctt acggctacca cccaacgcat ggttgagcag acgacggccc
    21121 aggcttacag aatcatcacg cttgaggttt ttcagcaact gacgaatttt ggcgcgcgct
    21181 ttcgagctga cgacaaagtt cagccacgcg gcattcgggc gagcacccgg tgcggtaatg
    21241 atttcaacgg tttgaccgct agtaagcggc tgcgacagcg ggtaaggctg gcggtcgacg
    21301 cgtgcgccca cgcaggcatg gccgatatcg gtatgcaccg cataagcgaa gtcgacgggc
    21361 gttgcaccgg caggcagttc gacaatgcgc ccttctggtg tgaaaacgta aatctcatcc
    21421 gggaagagat cggatttaac gctctcaata aattcaaacg agctaccggc actctgttgc
    21481 agctccagca ggctttgcat ccagcgctgg gcgcgtattt gcgcggtagt gctggtttcg
    21541 ccgtgctctt tataagccca gtgcgcggca acacccatct ccgccatctg atccatatct
    21601 tcggtacgga tctggacctc aaccggcacg ccgtgcgggc caatcatcga ggtgtgcaaa
    21661 gactgatagc cgttcgcttt tggaatggcg atatagtctt tcacgcggcc cggacgcggc
    21721 ttatacaggc tgtgcatctg gcccagcacg cgataacagg tgtcagaatc attgacgatc
    21781 acgcggaaag cgtagatgtc catgattgag tgaaaacgct gctctttgag caccattttg
    21841 cagtaaatcg aataaagatg cttttcgcga ccactgacgc ggcacggtat tcccgcttcc
    21901 tgcaaacgcc cttcgatttc agaaagaatc ttctggatca tctctttacg gttgccgcgc
    21961 gcggctttca ccacttcttt gattacgcga taacggttgg gatacagcgc ctcaaaaccc
    22021 agctcctcga gttcggtttt aatgtggtgg atacctaaac ggtgcgccag cgggctgtat
    22081 atttcgagag tttcacgggc gatgcggcga cgtttgtccg ggcgaagtga gcccagcgtg
    22141 cgcatgttgt gggtacggtc ggcaagtttg atgagaatga cgcggatatc ctgcaccatc
    22201 gccataatca tcttgcgaaa gttttcggcc tgcgcctctt tcttatcgcg gaacttgagt
    22261 ttatcaagtt tcgacacccc ctctaccagc tcggcgacgc ttttaccaaa aagctgttcc
    22321 atgtcctggt aggtggcggg tgtatcttca atcacatcat gcagcagcgc cgccatcagc
    22381 gtttcatagt cgagtttcat ctcggccaga atgcaggcaa ccgccaccgg atgcgtgata
    22441 tagggttcac cgcttgaacg tgtttgcccc tcgtgagcat cacgtgcaac gagatacgcc
    22501 tgccgcagac gcttgatttg gtcttccggc aggtagtttt gaatcagctg attcaggctt
    22561 tcaaacagat acaagggcga cccgctttgt gattaacgac gaccttcagc aatagcggta
    22621 acggcttgta attcagcggc ttcctgctct tgctgctcct ggcgttcgcg aacgtcgagg
    22681 atctggttgt tgatcagacc ttcttcgatt tcgcgcagcg cgattacagt ggttttatcg
    22741 ttttcttccg gtaccagcgg atcctttccg cctacctgca tctgacgagc gcgacgcgcg
    22801 gcgaccagta ccaggtcaaa acggttacca attttctcta cagcgtcctg aacagttacg
    22861 cgtgccatac ttaaaaagct ccacaggtga agaaatgact gggcatgata ctgaaatcag
    22921 gttcagtctg ccaacaattt gctgattaaa gcgtcatgac gctgcttttg gcggctcatg
    22981 cgcagacgtt cggcgcgaat aatggtcttc aaatcggtca acgcggtatc gaagtcatca
    23041 ttcacaatca ggtaatcata ttcggcgtaa tggctcattt ctgcaacagc ttgcgccata
    23101 cgctttgcaa tgacctcttc gctgtcctga ccgcgaccgc gtagacggcg gtccagttcg
    23161 attttagacg gcggtaaaat aaagatactc cgcgcgtgcg gcatcttctg gcgaatttgc
    23221 tgcgcaccct gccagtcgat atcgagaaaa acatcgacac cggtcgccag tacttgctca
    23281 atggcctcac gcgaagtgcc atagtaatta ccaaaaactt ctgcgtgttc gaggaacgca
    23341 tctctactaa tcatttcttt aaattcatca tgattaacaa agaaataatg ttcaccgtgg
    23401 acttcaccag gacgcggttg gcgtgtggtg tgtgaaacag aaacctgggt gtcatacaac
    23461 ggttgggttt ttaataaagc ctgaatcagg ctggatttac ccgcgccact gggggcagaa
    23521 acaatataaa gcgtgccttg agccatgagt atctttcgta tgtgattagc gaaataaagc
    23581 ctacatacga gcttattata cacggcgcgg ccatgtgacg tagcccttgc tcacactttt
    23641 tgcgcaaagt tgttgcgttt tgcgtagctg cttccgcatt tgcctgtgat tcactgcaat
    23701 aaagacgatt ctcaggaagc gtcctcgcag actccgtttt tgccactcgg ctttgccttc
    23761 accacattgt atatctgcct gactatctac agcggaggtg atgatgaaag tatggatggc
    23821 gatattaata agtatcttgt gctggcaatc atcggtgtgg gcggtctgtc cggcctggtc
    23881 gccagccaga gcacaggaag aaatttcccg cctgcaacag caaataaaac agtgggacga
    23941 tgactactgg aaggaaggga aaagtgaggt ggaagacggt gtttacgatc agttaagcgc
    24001 ccgtcttacg cagtggcaac gctgttttgg gaacgagacc cgcgatgtga tgataccgcc
    24061 tttaaacggt gcggttatgc atccggttgc ccatacgggt gtacgcaaaa tggcggataa
    24121 aaacgcatta agtctttgga tgcgcgaacg tagcgatctt tgggtgcagc caaaagttga
    24181 tggcgttgcg gtaaccctgg tttatcggga cgggaaactg aacaaagcaa tcagtcgcgg
    24241 caatggcctg aaaggcgagg actggacgca gaaagttcgc ttaatttccg ctgtgccgca
    24301 aaccgttagc gggcctttag ccaacagcac gcttcagggg gaaatatttc tcaagcgtaa
    24361 ggggcatatc caacaacaaa tggggggaat aaatgcccgc gcaaaagttg ctggcctgat
    24421 gatgcgccag gggaatagcg atacgctaaa ctcactggct gtttttgtct gggcatggcc
    24481 ggatggaccg catttaatga ccgatcgttt aaaggatctg gctaccgcag gttttacttt
    24541 aacgcagacg tatacccgcg cggttaaaaa tgctgatgag gttgcgcacg tacgcaatga
    24601 gtggtggaaa gcaaaattac ccttcgtcac cgatggcgtg gttgtgcgag cggcgaaaga
    24661 acccgaatcc cgtcattggc taccgggcca ggcggagtgg ctggtggcct ggaaatatca
    24721 acctgtagct caggttgccg aagtgaaggc gattcagttt gcggtgggta agagcggtaa
    24781 aatatcggtg gttgcgtcac ttgtacctgt catgctggat gataaaaaag tccagcgggt
    24841 gaatattggt tccgtcaggc gctggcagga gtgggatatt gcgcctggtg atcagatcct
    24901 cgtcagcctt gccggtcagg ggattcctcg cattgatgat gtggtctggc gcggagcaga
    24961 acgtacaaaa ccgacaccac cagaaaaccg ctttaactcg ttgacctgct actttgcttc
    25021 tgatgtttgt caggaacagt tcatttcacg cttagtctgg ctggggtcaa aacaggttct
    25081 ggggcttgat ggcattggtg aggccggctg gcgcgccctg catcagactc atcgctttga
    25141 gcatatcttt tcctggcttt tattaacgcc agagcaatta cagaacacgc cggggatcgc
    25201 gaaaagtaaa agtgcgcagt tatggcatca gtttaatctg gctcgtcagc agccttttac
    25261 tcgctgggtg atggcaatgg gaataccgct aactcgggcg gcgcttaatg ccagtgatga
    25321 acggtcctgg tcgcaacttt tatttagcac ggaacagttc tggcagcagc tgccggggac
    25381 tggatcgggg cgcgccagac aggttattga atggaaggaa aatgcgcaaa tcaagaagct
    25441 gggcagttgg ctggctgccc agcagatcac aggttttgaa ccttagtgac cttcgtggct
    25501 gtagtaaaag accggtaatc ccagcttaag acgtagcgcc agtaaacggg caaaaaagcc
    25561 gaataccagg gtggagataa tcaccacatc atggttagaa acatagtgtt gcagcgcgat
    25621 gtacagcacg gcggaggcaa atgagacacc cgcatacaac tctttctgga ataccagtgg
    25681 gatgcgttta cagaacatat cgcgcaaaac gccgccaaac acgccggtgg ttaccgccgc
    25741 gacaacggca ataatcgggc cgtgccccat atccagagca acctgtgcgc cgatgataga
    25801 aaataccacc agtccgagcg catcgagcac cagaaacact ttgcgcagat aaggcattac
    25861 aggagcaact atagtggtga gtactgcagc ggtagcgacg ataatcacat attccgggtg
    25921 tttgacccag ccgagcggat agtggcccag cagaatatcg cgcactgacc ctccgccaat
    25981 tgccgtggcg gtcgcaataa taattacgcc aaatgtatcc atgcgccgtc gtccggccgc
    26041 cagcgccccc gtcatggctt cggcagtaat gccaaccaaa tacaaaatgt gtaacagcat
    26101 ggttctctcc ctaaaaatct gtctggaagg gtagcgattt gtgcgcattg tcacgattga
    26161 gattttttaa ggagagaata gattgataat ttgtagttat gtgaaattgc tattttctgt
    26221 agtaagaatg catttttctg attttgtgat aagtaaaatg cattagaaaa agtatggagt
    26281 aaataaatat gaggacgaaa ctatatgcgt agcgccgctg taattaacaa cggcctacgg
    26341 agatatgtcg cttactcgat attctgaatc tgctcgcgca tctgttcaat caacactttc
    26401 agctcgatgg cggagtttgt cacttcggcg ttgatagatt tcgacgcaag agtgttcgac
    26461 tcgcggttga actcctgcat cataaagtcc agacgacgac caacggcttc ttttttcttc
    26521 agaatgttgt aggtctcttt gacatgcgct tcgaggcggt ccagttcttc ggcaacgtca
    26581 attcgttgtg ccagcagaac caactcctgc tccagacggt tgttttccag ttgcacctga
    26641 gcatcttcca gcttcgctac cagacgctca cgctgccatt gcaggatttc cggcatatgg
    26701 gcgcggactt tgaccacttc ggcggtgacg ccttccagac gctgctcaat caacactttc
    26761 agcgcctgac cttcggtttc gcgcgcgatg ataaagtcat ccagggtgcc atccaacgcc
    26821 gcgagaattt cagctgcaat cgcgtcgaga tcttgctcct gggctgccat cacgcccggc
    26881 cagcgtagaa tatcaaccgg gttgatttct ccttcgtcgc tctgcatttt tacccagttc
    26941 gcggcagtta ccagctgttt agccagtttt tcgttgagga tcagctcacc ttgcgcgcta
    27001 acatctggct catagcgcag agtacattcc actttaccgc gcgtcaggcg agaacgaata
    27061 cgctcgcgaa cgacaggttc aaggctacgg aactgctccg gcagacgaaa gtaagtttcc
    27121 agataacgct ggtttaccga gcgcatttcc caggttgcgc tcccccattc acccttgatt
    27181 tcacgccggg cgtaggcggt catactgcgg atcatagacg ttcctgttta taaaaggaga
    27241 ggtggaagga ttatagccat cgatgccttg tcaggatagg aataaccgcc ggaagtccgt
    27301 ataatgcgca gccacatttg tttcaagccg gagatttcaa tatgcgtcca gcaggccgta
    27361 gcaataatca ggtgcgtccc gttaccctga ctcgtaacta tacaaaacat gcagaaggct
    27421 cggtgctggt cgaatttggc gataccaaag tattgtgtac cgcctctatt gaagaaggcg
    27481 tgccgcgctt cctgaaaggt cagggccagg gctggatcac cgcagagtac ggtatgctgc
    27541 cgcgttctac ccatacccgt aacgcgcgtg aagcggcgaa aggtaagcag ggtggacgca
    27601 caatggaaat ccagcgtcta atcgcccgtg ctcttcgcgc ggcagtagat ttgaaagcac
    27661 tgggtgagtt caccattacg ctggactgtg acgtgcttca ggctgatggt ggcacgcgta
    27721 ccgcttcgat tacgggtgcc tgcgtggcgc tggcagatgc gctacagaag ctggtggaaa
    27781 acggcaagct gaaaactaat ccaatgaaag ggatggtagc cgcggtctct gtcgggattg
    27841 tgaacggtga agcggtttgc gatctggaat atgttgaaga ctctgccgca gagaccgaca
    27901 tgaacgtagt gatgaccgaa gacgggcgta tcattgaagt gcaggggacg gcagaaggcg
    27961 aaccgttcac ccacgaagag ttactcacct tgttggcgct ggcccgaggg ggaatcgaat
    28021 ccattgtagc gacgcagaag gcggcgctgg caaactgatt tttaaggcga ctgatgagtc
    28081 gccttttttt tgtctgtaga aaagtaagat gaggagcgaa ggcatgaaac catatcagcg
    28141 ccagtttatt gaatttgcgc ttagcaagca ggtgttaaag tttggcgagt ttacgctgaa
    28201 atccgggcgc aaaagcccct atttcttcaa cgccgggctg tttaataccg ggcgcgatct
    28261 ggcactgtta ggccgttttt acgctgaagc attggtggat tccggcattg agttcgatct
    28321 gctgtttggc cctgcttaca aagggatccc gattgccacc actaccgccg tggcgctggc
    28381 ggagcatcat gaccttgacc taccgtactg ctttaaccgc aaagaggcaa aagaccacgg
    28441 tgaaggcggt aatctggttg gtagcgcgtt acaaggacgc gtaatgctgg tagatgatgt
    28501 gatcaccgcc ggaacggcga ttcgcgagtc gatggaaatt attcaggcca atggcgcgac
    28561 gcttgcaggc gtgttgattt cgcttgatcg tcaggaacgc gggcgcggcg aaatttcggc
    28621 gattcaggaa gttgagcgtg attacaactg caaggtgatc tctatcatca ccctgaaaga
    28681 cctgattgct tacctggaag agaagccaga aatggcggaa catctggcgg cggttaaggc
    28741 ctatcgcgaa gagtttggcg tttaacgaaa ctcgccggat gaaaagtcat ccggcgtcat
    28801 attactgcaa ctgtgcggca attagcggcc agcgggcgtc aaaatcatcc gtcgggcggt
    28861 atttaaactc gctgcggaca aatcgtgaca gcataccttc acaaaatgcc agcaactggc
    28921 ttgccagcag ggtttcatcg gtggtgtaac cttcaccctc acgcattctc ttttcacgca
    28981 atacctgacg caactgcgct tcaatacgct cgaacagctg gttgatgcgc ccttgcagac
    29041 gatcctgttc gaacattagc gcatgaccag tgaggatgcg agtcaggcca ggattacgct
    29101 caccaaaacc gaggagcaac aacacgatca gacgcaggcg cgctgtggtg tctttctcat
    29161 ctttcagaat caggttgata cgagtaatca ggctatcttc gataaactca atcaggctat
    29221 cgaacatgcg ggtcttactg gggaagtggc gatacagtgc cgcttcggaa acgccgacag
    29281 aggcggccag ttttgccgtc gtgatacgtt ggcttccatc gctggattcc agcatcagcg
    29341 ccagagactg aagtatttcc tcgcgacggt tccttttcgc agtttgtttt tctgccatgt
    29401 tacaaaatac ccctgaaaat aagcacttgc caggcggcac ccacgctatg accgcaaacg
    29461 aaatgtttgc ggctatgtta tgacgttatt cggatgcgta tgtgttactg acgaccagag
    29521 tgaccaaagc cgccttcacc gcggtcggtg gcgtcgaaat cttccaccag attaaattca
    29581 gcctgtacta ccggaacaaa aatcatctgg gcgatgcgtt cgccaggttg aatggtgaag
    29641 ctgtcctgac cacggttcca cacggaaatc atcaactggc cctgatagtc agaatcgatc
    29701 aatcctacca ggttaccaag cacgataccg tgcttatgtc ccaatccgga gcgcggtagc
    29761 atcattgccg ccagtgaagg atcggcaata tgaatcgcca gcccggtcgg aaccagcgta
    29821 gtgtcacccg gagccagttc tacggcgtcg tcgagacagg cacgcaggtc aagtccggca
    29881 gagccagagg tggcataagt cgggagcgga aattccttcc caacgcgcgg gtccagaatc
    29941 ttaacgtcga tttttttcat cataacgggt cacgatctcg tcgagtaata attggccaag
    30001 gagctctttg cgctcaagcg gtaagacttt atctccgtcc tgccagaaaa ggtgtaatgc
    30061 gttgttgtcg ctgttaaatc cttgagttgg ctgggaaaca tcgttcgcgc agatcagatc
    30121 aaggttttta cggatacgtt tttgccgggc gtattcttcc acattatttg tttcggcggc
    30181 aaatccaacg acgtaaggtc gatggtcttt tagtgcggca acgcctgcga cgatatcggg
    30241 gtttttaacc atttttattg ttaattcatc accctgcgtg gcctgctttt tgattttttc
    30301 tggggccacg gtagctgcgc gataatccgc cacggcggcg caaccgataa aaatattttg
    30361 ctgctgtact gaagcattca cggcggcttc catttccagc gcggtcatca catcaacacg
    30421 gttaacaaac ggtggcgtcg gtagtgaaac cggacctgat accagcgtga cgttcgcgcc
    30481 acgacgggct gcggcggcgg cgatagcaaa acccatcttg ccggagctgt gattagagat
    30541 ataacgcacg ggatcgagcg gttcacgcgt cggaccggcg gtaatcataa tgttcagatg
    30601 tttcaggtcg ttgacgggcg aaaaatgcgc taccgccata tccacaatag ttaacggatc
    30661 gagcattcgt cctgggccga tatcaccaca agcctgactg ccgctgtctg gcccccagat
    30721 gagcaaacca cgtgaggcaa gcacctctaa attatgctgg gtggcggcgg cacggtacat
    30781 ctgctggttc atggcgggga gcacggctac aggcgcaggt gtagccagac aaatcgtcga
    30841 taccaggtca ttcgccattc cggctgcaac acgggcaatt aaatctgccg tggcaggggc
    30901 gagaatcact aaatcagccc attttcccag ctcaatatgg cccatagcgg cttcggctgc
    30961 cgggtccagc agactgtcgg aaacgggata accagaaacc gcctgcaagc taagtggggt
    31021 gataaaggct tttgccgctt cggtcatggc tacgcagaca tcggccccgc gatcgcgcaa
    31081 acgacgcacc agttcagggg ttttataggc agcaataccg ccgctaacgc cgagaacgat
    31141 ttttttaccg gccaggctca tgatgatttt tcctgttggg tgacaccaga agttggcgat
    31201 tttatcacaa tacttttgtt gtcgtgcctt cacctgagat tcactttgcg aggcgctttc
    31261 caggattgaa aactggccgt cgatttaaag gaacggctat gacaggatgc gagcaccaca
    31321 aaggaggtga aggtgaaaaa caatgtacag ctgttgatgc cgcgcgaaaa aatgctgaag
    31381 tttggtatta gcgccttaac ggatgtcgag ctgctggcgc tatttctgcg taccggaacg
    31441 cgcggtaaag atgtattaac cctggcaaaa gagatgctgg agaatttcgg ctctctttat
    31501 ggcttgttaa cctctgaata tgagcaattt agtggcgttc atggaattgg cgtggcgaaa
    31561 ttcgcccagt taaaggggat tgccgaactg gcgcggcgtt actacaacgt acggatgcgt
    31621 gaagaaagcc ctttactcag cccggagatg acgagggaat ttttacaaag ccagctcacg
    31681 ggcgaggagc gggagatctt tatggtgatc tttctcgact cccaacacag ggttataacg
    31741 catagccgtc ttttttccgg cacgctaaac catgttgaag tccatcctcg ggaaattatc
    31801 cgcgaagcga taaaaataaa cgcctcggcg ctgatccttg cgcataatca cccttcgggt
    31861 tgtgctgaac ccagtaaagc ggataaactc attactgaac ggataataaa gagttgtcag
    31921 ttcatggatt tacgcgtgct cgaccatatc gtgattgggc gtggagagta tgtttctttt
    31981 gccgaacgcg gctggattta acccgctatg cgcgatcctt cgggatcttt gtctgttcgg
    32041 gacttgagca catcgctgag tcagcgtata ctacgccacc tttgagaatc tcgggtttgg
    32101 catttgggcc tggcaatcga gagttcacat agaactgcga tgaccgggct gtaaagcctg
    32161 acgaggcgcc aataccccat acgaagctcg agctaatttg atttttggag aatagacatg
    32221 tcccgagtct gccaagttac tggcaagcgt ccggtgaccg gtaacaaccg ttcccacgca
    32281 ctgaacgcga ctaaacgccg tttcctgccg aacctgcact ctcaccgttt ctgggttgag
    32341 agcgagaagc gttttgtcac cctgcgcgta tctgctaaag gtatgcgtgt aatcgataaa
    32401 aaaggcatcg atacagttct ggctgaactg cgtgcccgtg gcgaaaagta ctaagtactt
    32461 agaggaaata aatcatggct aaaggtattc gtgagaaaat caagctggtt tcttctgctg
    32521 gtactggtca cttctatacc actacgaaga acaaacgtac taagccggaa aaactggaac
    32581 tgaaaaaatt cgatccagtt gttcgccagc acgtgatcta caaagaagcg aaaatcaaat
    32641 aatttccgct ttgatgtaac aaaaaacctc gccccggcgg ggttttttgt tatctgcttg
    32701 cccccatatt gactgcatct gttcattcct ggagatgcta tgcctgaatt acccgaagtt
    32761 gaaaccagcc gtcgcggcat agaaccgcat ctcgttggtg caaccattct tcatgcggtg
    32821 gtgcgcaacg gacgcttgcg ctggccggtt tcagaagaga tctaccgttt aagcgaccaa
    32881 ccagtgctta gcgtgcagcg ccgggctaaa tatctgctgc tggagctgcc tgagggctgg
    32941 attatcattc atttggggat gtctggcagc ctgcgcatcc ttccagaaga acttccccct
    33001 gaaaagcatg accatgtgga tttggtgatg agcaacggca aagtgctgcg ctacaccgat
    33061 ccgcgccgct ttggtgcctg gctatggacc aaagagctgg aagggcacaa cgtgctggcc
    33121 catcttggac cggagccgct tagtgacgat ttcaacggtg agtacctgca tcagaagtgc
    33181 gcgaagaaaa aaacggcgat taaaccgtgg ctgatggata acaagctggt ggtaggcgta
    33241 gggaatattt atgccagcga atcgctgttt gcggcgggga tccatccgga tcggctggcg
    33301 tcatcactgt cgctggcgga gtgtgaattg ttagctcggg tgattaaagc ggtgttgctg
    33361 cgttcgattg agcagggcgg tacaacgctg aaagattttc tgcaaagtga tggtaaaccg
    33421 ggctatttcg ctcaggaatt gcaggtttac gggcgaaaag gtgagccgtg tcgggtgtgc
    33481 ggtacgccga ttgtggcgac taaacatgcg cagcgggcaa cgttttattg tcggcagtgc
    33541 cagaagtaat tgatgcgcgc cggatggcat accatccggc ataaactcta cgctaacttc
    33601 gccatcagcg cctgatggac attctccggc aggaaatggg tgacatcgcc ctgatggcgc
    33661 gccacctctt tcaccaacga tgaagagata aacgaccact ctttcgacgg catcagaaac
    33721 acactttcca gctccggcat taagtggcga ttcatatgcg ccagctgcat ttcatattca
    33781 aaatccgcca ccgcacgcag gccacgaatc agcaccgtag cgtgttgatt acgggcgaag
    33841 ttcgccatca aatcactaaa cccgaccact tccacgttct ccagatgcgc ggttgcctgc
    33901 tgtgccagct ccacacgctc ttccagggta aacatcggtt ttttactggg actagcggca
    33961 atcgccagaa taacgtgatc gaacatttgc gaggcgcgcg tcacgatatc gatatgacca
    34021 ttggtaatgg gatcgaaagt acccggataa atcgcccgtt tttgcataac aacctcaatg
    34081 cgttttcggt ggcaggtaag gttccagcag ttgaagcaga cgctgtagtg cgccctggtt
    34141 ttgatacagc acttcaacgg catgacggcc atagaaacta cggtaatcgg cgtcggtgag
    34201 taaagaggaa acctcttttg caagcgtagt ggcatcggta acggtaatca gcccgcttgc
    34261 ctgctccagc cgcgcgcaaa tgtctttaaa gttaaacgta tgcggcccca tcaataccgg
    34321 aatagcgtgt gcggccgctt ccagcggatt atgcccacca cgttcaacca gtgaaccgcc
    34381 aacaaaggcg agatcggcaa tgccgtacag taacatcaac tcgcccatcg tatcgccaac
    34441 cacaacctgc gtgctggtgg aggggacttc ccctgaagat cgtgtgatat agcttagtcc
    34501 agcctggcgg acaaggttaa tcgcatccgg gaagcgttct ggatgacggg gtaccaggat
    34561 gagcaataaa ttcgggaatt gctgtaacaa tgcctgatgt gcggcgatca ccacactctc
    34621 ttcgccttca tgagtgctgg tggcaatcca taccgggcgg tgtggtgccc actggcggcg
    34681 cagcgtcacg gctttagcag ccaactgcgg cgttacagaa atatcgaatt tcaggctacc
    34741 ggtaacggtc acctgattat tttttgcgcc cagcgccaca aaacgtgcac catcttcttc
    34801 attttgggcg gcaatcagcg taatacgacg cagcaagcgg cggacgaatt tacccagttt
    34861 ggcataacct gcggccgagc gggcagagag tcgcgcgtta gcgatcacca gcggaatttt
    34921 acgtttatgt agcgccgcaa tcaggttagg ccatagttcg gtttccataa tcaacaccag
    34981 tttagggtcg actttattca ggaaacggtt gagtgcatcg ggcaggtcat acggcagata
    35041 aacgtgctga acatccttcc cgaaagccga ttgtacgcgc tccgaaccgg ttggagtcat
    35101 ggttgttacg gtaatcggta aatcaggata acgatgacgc agtgcgcgca ccaacgggat
    35161 cgccgccaga gtttcaccga cggagacgga gtgcagcata atgccgcctg gttttagcgg
    35221 atggcggtaa aaaccgtaac gttcacccca gcgttttcga taggccggag ccttacgtcc
    35281 gcgcacccag agccgtatcc agatcagtgg ctgaataagg tagagaaggg cggtgtaaag
    35341 caattcgagc atagtaaata gctgacttat ggatgtgctg gggattctat gtatttagct
    35401 gtggctttac cattactttt cccgtttttg acttaaatag caatttaccc gccaattctt
    35461 gctgaacatc tatacttttt ttgtattttt atgcgattca ttgatttctg cagtacgtgc
    35521 aggaaggaat cgcagagcaa cagtaaaaag aatatggttg ctaatgattt tatttgattg
    35581 atttctttat tgaaaatcaa caatgtgctt aaaaatcatt cgggtcggat tgccgcgaaa
    35641 gaagtgatac actagcgcgt caatatcagt gcggtatcag tattcaggta gctgttgagc
    35701 ctggggcggt agcgtgcttt ttctgcttca ccttaatcgg ataatctcaa caaaaagagt
    35761 tacttgtgga taagccattt cgaagaattt tgctcattaa gatgcgtttt catggggata
    35821 tgttattaac tactcccgtc attagttcgc tgaaaaaaaa ttaccctgac gcaaaaatcg
    35881 atgtgctgct ttatcaggac accatcccga tcctgtctga aaatccagag attaacgcgc
    35941 tctacggcat aaaaaataaa aaagcaaaag cctcagaaaa aattgccaac ttttttcatc
    36001 tcatcaaggt attacgtgcc aataagtatg accttatcgt taatcttacc gatcaatgga
    36061 tggttgctat actggttcgc ttattaaatg cccgtgtgaa aatttcccag gattatcatc
    36121 atcggcagtc tgctttttgg cgtaatagtt tcactcattt ggtgccgttg cagggtggaa
    36181 atgtggtgga aagtaactta tccgtgctga cgccattggg acttgaatcg ttggtgaagc
    36241 agacaaccat gagttacccg cctgcaaact ggaaacgtat gcgtcgcgaa cttgatcacg
    36301 ctggtgttgg acaaaattat gtggttatcc aacctacggc gcggcaaatc ttcaaatgct
    36361 gggacaacgc caagttttcc gctgtgattg atgccttaca tgctcgtggt tatgaagtcg
    36421 ttctgacgtc cggcccagat aaagacgatc tggcctgcgt caatgaaatt gcgcagggat
    36481 gccagacgcc accagtaacg gcgctggccg gaaaggtgac cttcccggaa cttggtgcgt
    36541 taatcgatca tgcgcagctg tttattggcg ttgattccgc accggcgcat attgccgctg
    36601 cagttaatac gccgctgata tcgctgtttg gtgcgacgga ccatattttc tggcgtccct
    36661 ggtcaaataa catgattcaa ttctgggcgg gagattaccg ggaaatgcca acgcgcgatc
    36721 agcgtgaccg aaatgagatg tatctttcgg ttattccggc ggcagatgtc attgcagctg
    36781 tcgataaatt acttccctcc tccacgacag gtacgtcgtt atgatcgttg ctttttgttt
    36841 atataaatat tttccctttg gcggtttgca gcgcgatttt atgcgtattg ctcagacagt
    36901 cgccgcccga ggccatcatg ttcgggttta tacccagtcg tgggaaggcg aatgccctga
    36961 tgtatttgaa ctgatcaaag tgccggttaa atcgcatacc aatcacgggc gcaatgcgga
    37021 atattttgcc tgggtgcaaa aacatttacg tgaacacccc gtcgatagag ttgttggatt
    37081 taataaaatg ccggggctgg acgtttatta tgccgctgat gtttgttatg ccgagaaagt
    37141 agcgcaggag aaaggctttt tctatcgcct gacgtcacgt tatcgccatt atgctgcctt
    37201 tgagcgggca accttcgaac agggcaagcc gacacagctg ctgatgctga cagataagca
    37261 aatcgccgat ttccagaaac attatcagac tgaagcggaa cgttttcata ttctgccacc
    37321 ggggatttat cctgatcgta aatatagcca gcagccagcc aatagccgtg aaatcttccg
    37381 taagaagaat ggaataaccg aacaacaata tttattgttg caggttggtt cagacttcac
    37441 gcgtaaaggt gtcgatcgtt ccattgaagc acttgcttcg ttaccggatt cgctgcgcca
    37501 caacacattg ctatatgttg ttgggcagga taaaccgcga aaatttgagg cgctggcaga
    37561 aaaacgcggc gtgcgcagta atgttcactt cttctcgggg cgcaacgatg tctcggaatt
    37621 aatggcggcg gcggatttat tacttcatcc tgcctaccag gaagccgcgg gaattgtact
    37681 gctggaagcg ataactgcag gattaccggt actaacaaca gccgtttgtg gctatgcgca
    37741 ttatattgtt gacgccaatt gcggcgaggc tattgctgag ccattccgcc aggaaacatt
    37801 gaatgagatt ttacgcaaag cgttaacgca atcttcattg cgccaggcct gggcggaaaa
    37861 tgcgcgacat tatgctgata cacaagattt atacagtctg ccagagaaag cggcggacat
    37921 cataacgggt ggtctggatg gttgaactta aagagccgtt tgccacgtta tggcgcggta
    37981 aagatccttt tgaggaagtt aaaaccttgc agggtgaggt atttcgtgaa ctggaaactc
    38041 gccgcactct gcgctttgaa atggcgggca aaagctattt tctcaaatgg catcgcggca
    38101 cgaccctgaa agagataatc aaaaatttac tctcattgcg gatgccagta ttaggcgcag
    38161 accgcgaatg gaatgcgatt catcgactgc gggatgtcgg cgttgatact atgtatgggg
    38221 tggcattcgg cgaaaaaggc attaatccgc tgaccagaac ctcgtttatt attaccgaag
    38281 atctgacacc aaccatcagt ctggaagatt actgtgctga ctgggcgacc aacccaccag
    38341 atgttcgcgt gaagcgtatg cttattaagc gtgtcgcgac gatggtgcgc gatatgcatg
    38401 ctgcgggcat taaccaccgt gactgttata tctgtcattt cctgctgcac ttgccttttt
    38461 ccggtaagga agaggagtta aaaatttcag taattgacct gcaccgggcg cagatacgtg
    38521 caaaagtacc gcgtcgttgg cgggataaag atttgattgg tttgtatttt tcatcaatga
    38581 atattgggct gacgcagaga gatatctggc gatttatgaa ggtttatttt gggatgcctt
    38641 tacgtaagat attatctttt gaacggaatt tattgaacat ggcaagtgtt aaggccgagc
    38701 atattaaaga gcgaacacaa cgcaaaggat tataaaaata ttaatcagta tgtactggaa
    38761 ctaacttcat tatgactatt tattttataa actgggttgc agattatgaa ttaaaaatga
    38821 tccagtactt gaaaaaaaag cacaagataa aaaatataac aacaccaaaa aaatacaact
    38881 gggttaataa gaaaatttcg aaattaggga tggataatgc atggttggga cgattattta
    38941 tcaaacatca cctaaatgct gtaaaaaagg atgatatcat tatatttaat gacagtgtca
    39001 ttaataaaag tattaacaag caaatattaa aaaatat
//
LOCUS       DAHHTG010000034        37481 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.034, whole genome shotgun sequence.
ACCESSION   DAHHTG010000034 DAHHTG010000000
VERSION     DAHHTG010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 37481)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37481)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37481
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.034"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            102..1046
                     /gene="leuO"
                     /locus_tag="NBI74_001700"
     CDS             102..1046
                     /gene="leuO"
                     /locus_tag="NBI74_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LeuO"
                     /protein_id="HCD1932058.1"
                     /translation="MPEVQTDHPETAELSKPQLRMVDLNLLTVFDAVMQEQNITRAAH
                     ALGMSQPAVSNAVARLKVMFNDELFVRYGRGIQPTARAFQLFGSVRQALQLVQNELPG
                     SGFEPASSERVFHLCVCSPLDSILTSQIYNHIEQIAPNIHVMFKSSLNQNTEHQLRYQ
                     ETEFVISYEDFHRPEFTSVPLFKDEMVLVASKNHPTIKGPLLKHDVYNEQHAAVSLDR
                     FASFSQTWYDTVDKQASIAYQGMAMMSVLSVVSQTHLVAIAPRWLAEEFAESLELQVL
                     PLPLKQNSRTCYLSWHEAAGRDKGHQWMEEQLVSICKR"
     gene            complement(1207..1245)
                     /locus_tag="NBI74_001701"
     CDS             complement(1207..1245)
                     /locus_tag="NBI74_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1932059.1"
                     /translation="MKRNDKILHYRG"
     gene            1364..3088
                     /gene="ilvI"
                     /locus_tag="NBI74_001702"
     CDS             1364..3088
                     /gene="ilvI"
                     /locus_tag="NBI74_001702"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020079613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 3 large subunit"
                     /protein_id="HCD1932060.1"
                     /translation="MEMLSGAEMVVRSLIDQGVKQVFGYPGGAVLDIYDALHTVGGID
                     HVLVRHEQAAVHMADGLARATGEVGVVLVTSGPGATNAITGIATAYMDSIPLVVLSGQ
                     VATSLIGYDAFQECDMVGISRPVVKHSFLVKQTEDIPQVLKKAFWLAASGRPGPVVVD
                     LPKDILNPANKLPYVWPESVSMRSYNPTTSGHKGQIKRALQTLVAAKKPVVYVGGGAI
                     TAGCHLQLKEAVEVLNLPVVSSLMGLGAFPATHRQALGMLGMHGTYEANMTMHNADVI
                     FAVGVRFDDRTTNNLAKYCPNATVLHIDIDPTSISKTVTADIPIVGDARQVLEQMLEL
                     LSQESAHQPLDEIRDWWQQIEQWRARQCLKYDTHSEKIKPQAVIETLWRLTNGDAYVT
                     SDVGQHQMFAALYYPFDKPRRWINSGGLGTMGFGLPAALGVKMALPEETVVCVTGDGS
                     IQMNIQELSTALQYELPVLVVNLNNRYLGMVKQWQDMIYSGRHSQSYMQSLPDFVRLA
                     EAYGHVGIQISHPQELESKLSEALEQVRNNRLVFVDVTVDGSEHVYPMQIRGGGMDEM
                     WLSKTERT"
     gene            3091..3582
                     /gene="ilvN"
                     /locus_tag="NBI74_001703"
     CDS             3091..3582
                     /gene="ilvN"
                     /locus_tag="NBI74_001703"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HCD1932061.1"
                     /translation="MRRILSVLLENESGALSRVIGLFSQRGYNIESLTVAPTDDPTLS
                     RMTIQTVGDEKVLEQIEKQLHKLVDVLRVSELGQGAHVEREIMLVKIQASGYGRDEVK
                     RNTEIFRGQIIDVTPSLYTVQLAGTSDKLDAFLASIRDVAKIVEVARSGVVGLSRGDK
                     IMR"
     gene            3594..3680
                     /locus_tag="NBI74_001704"
     CDS             3594..3680
                     /locus_tag="NBI74_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fruR/shl operon leader peptide"
                     /protein_id="HCD1932062.1"
                     /translation="MRNLQPNMSRWAFFAKSVGTWNKSSCRS"
     gene            3762..4766
                     /gene="cra"
                     /locus_tag="NBI74_001705"
     CDS             3762..4766
                     /gene="cra"
                     /locus_tag="NBI74_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite repressor/activator"
                     /protein_id="HCD1932063.1"
                     /translation="MKLDEIARLAGVSRTTASYVINGKAKQYRVSDKTVEKVMAVVRE
                     HNYHPNAVAAGLRAGRTRSIGLVIPDLENTSYTRIANYLERQARQRGYQLLIACSEDQ
                     PDNEMRCIEHLLQRQVDAIIVSTSLPPEHPFYQRWANDPFPIVALDRALDREHFTSVV
                     GADQDDAEMLAEELRKFPAETVLYLGALPELSVSFLREQGFRTAWKDDPREVHFLYAN
                     SYEREAAAQLFEKWLETHPMPQALFTTSFALLQGVMDVTLRRDGKLPSDLAIATFGDN
                     ELLDFLQCPVLAVAQRHRDVAERVLEIVLASLDEPRKPKPGLTRIKRNLYRRGVLSRS
                     "
     gene            5368..5826
                     /gene="mraZ"
                     /locus_tag="NBI74_001706"
     CDS             5368..5826
                     /gene="mraZ"
                     /locus_tag="NBI74_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HCD1932064.1"
                     /translation="MFRGATLVNLDSKGRLSVPTRYREQLLENAAGQMVCTIDIHHPC
                     LLLYPLPEWEIIEQKLSRLSSMNPVERRVQRLLLGHASECQMDGAGRLLIAPVLRQHA
                     GLTKEVMLVGQFNKFELWDETTWHQQVKEDIDAEQLATGDLSERLQDLSL"
     gene            5828..6769
                     /gene="rsmH"
                     /locus_tag="NBI74_001707"
     CDS             5828..6769
                     /gene="rsmH"
                     /locus_tag="NBI74_001707"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HCD1932065.1"
                     /translation="MMENYKHTTVLLDEAVNGLNIRPDGIYIDGTFGRGGHSRLILSQ
                     LGEEGRLLAIDRDPQAIAVAKTIDDPRFSIIHGPFSALGEYVAERDLIGKIDGILLDL
                     GVSSPQLDDAERGFSFMRDGPLDMRMDPTRGQSAAEWLQTAEEADIAWVLKTYGEERF
                     AKRIARAIVERNREQPMTRTKELAEVVAAATPVKDKFKHPATRTFQAVRIWVNSELEE
                     IEQALKSSLNVLAPGGRLSIISFHSLEDRIVKRFMRENSRGPQVPAGLPMTEEQLKKL
                     GGRQLRALGKLMPGEEEVAENPRARSSVLRIAERTNA"
     gene            6766..7131
                     /gene="ftsL"
                     /locus_tag="NBI74_001708"
     CDS             6766..7131
                     /gene="ftsL"
                     /locus_tag="NBI74_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HCD1932066.1"
                     /translation="MISRVTEALSKVKGSMGSHERHALPGVIGDDLLRFGKLPLCLFI
                     CIILTAVTVVTTAHHTRLLTAQREQLVLERDALDIEWRNLILEENALGDHSRVERIAT
                     EKLQMQHVDPSQENIVVQK"
     gene            7147..8913
                     /gene="ftsI"
                     /locus_tag="NBI74_001709"
     CDS             7147..8913
                     /gene="ftsI"
                     /locus_tag="NBI74_001709"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000642204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase FtsI"
                     /protein_id="HCD1932067.1"
                     /translation="MKAAAKTQKPKRQEEHANFISWRFALLCGCILLALAFLLGRVAW
                     LQVISPDMLVKEGDMRSLRVQQVSTSRGMITDRSGRPLAVSVPVKAIWADPKEVHDAG
                     GISVGDRWKALANALNIPLDQLSARINANPKGRFIYLARQVNPDMADYIKKLKLPGIH
                     LREESRRYYPSGEVTAHLIGFTNVDSQGIEGVEKSFDKWLTGQPGERIVRKDRYGRVI
                     EDISSTDSQAAHNLTLSIDERLQALVYRELNNAVAFNKAESGSAVLVDVNTGEVLAMA
                     NSPSYNPNNLSGTPKEAMRNRTITDVFEPGSTVKPMVVMTALQRGVVRENSVLNTVPY
                     RINGHEIKDVARYSELTLTGVLQKSSNVGVSKLALAMPSSALVDTYSRFGLGKATNLG
                     LVGERSGLYPQKQRWSDIERATFSFGYGLMVTPLQLARVYATIGSYGIYRPLSITKVD
                     PPVPGERVFPESIVRTVVHMMESVALPGGGGVKAAIKGYRIAIKTGTAKKVGPDGRYI
                     NKYIAYTAGVAPASQPRFALVVVINDPQAGKYYGGAVSAPVFGAIMGGVLRTMNIEPD
                     ALTTGDKNEFVINQGEGTGGRS"
     gene            8900..10387
                     /gene="murE"
                     /locus_tag="NBI74_001710"
     CDS             8900..10387
                     /gene="murE"
                     /locus_tag="NBI74_001710"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HCD1932068.1"
                     /translation="MADRNLRDLLAPWVPDAPSRALREMTLDSRVAAAGDLFVAVVGH
                     QADGRRYIPQAIAQGVAAIIAEAKDEATDGEIREMHGVPVIYLSQLNERLSALAGRFY
                     HEPSDNLRLVGVTGTNGKTTTTQLLAQWSQLLGETSAVMGTVGNGLLGKVIPTENTTG
                     SAVDVQHELAGLVDQGATFCAMEVSSHGLVQHRVAALKFAASVFTNLSRDHLDYHGDM
                     EHYEAAKWLLYSAHHCGQAIINADDEVGRRWLAKLPDAVAVSMEDHINPNCHGRWLKA
                     TDVNYHDSGATICFSSSWGDGEIESRLMGAFNVSNLLLALATLLALGYPLADLLKTAA
                     RLQPVCGRMEVFTAPGKPTVVVDYAHTPDALEKALQAARLHCAGKLWCVFGCGGDRDK
                     GKRPLMGAIAEEFADVVVVTDDNPRTEEPRAIINDILAGMLDAGYAKVMEGRAEAVTC
                     AVMQAKENDVVLVAGKGHEDYQIVGNQRLDYSDRVTVARLLGVIA"
     gene            10384..11742
                     /gene="murF"
                     /locus_tag="NBI74_001711"
     CDS             10384..11742
                     /gene="murF"
                     /locus_tag="NBI74_001711"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HCD1932069.1"
                     /translation="MISVTLSQLTDILNGELQGADITLDAVTTDTRKLTPGCLFVALK
                     GERFDAHDFADQAKAGGAGALLVSRPLDIDLPQLIVKDTRLAFGELAAWVRQQVPARV
                     VALTGSSGKTSVKEMTAAILSQCGNTLYTAGNLNNDIGVPMTLLRLTPEYDYAVIELG
                     ANHQGEIAWTVSLTRPEAALVNNLAAAHLEGFGSLAGVAKAKGEIFSGLPENGIAIMN
                     ADNNDWLNWQSVIGSRKVWRFSPNAANSDFTATNIHVTSHGTEFTLQTPTGSVDVLLP
                     LPGRHNIANALAAAALSMSVGATLDAIKAGLVNLKAVPGRLFPIKLAENQLLLDDSYN
                     ANVGSMTAAVQVLAEMPGYRVLVVGDMAELGAESEACHVQVGEAAKAAGIDCVLSVGK
                     QSHAISTASGVGEHFSDKTALIARLKSLIAEQQVITILVKGSRSAAMEEVVRALQENG
                     TC"
     gene            11736..12818
                     /gene="mraY"
                     /locus_tag="NBI74_001712"
     CDS             11736..12818
                     /gene="mraY"
                     /locus_tag="NBI74_001712"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HCD1932070.1"
                     /translation="MLVWLAEHLVKYYSGFNVFSYLTFRAIVSLLTALFISLWMGPRM
                     IAHLQKLSFGQVVRNDGPESHFSKRGTPTMGGIMILTAIVISVLLWAYPSNPYVWCVL
                     VVLVGYGVIGFVDDYRKVVRKDTKGLIARWKYFWMSVIALGVAFALYLAGKDTPATQL
                     VVPFFKDVMPQLGLFYILLAYFVIVGTGNAVNLTDGLDGLAIMPTVFVAGGFALVAWA
                     TGNMNFASYLHIPYLRHAGELVIVCTAIVGAGLGFLWFNTYPAQVFMGDVGSLALGGA
                     LGIIAVLLRQEFLLVIMGGVFVVETLSVILQVGSFKLRGQRIFRMAPIHHHYELKGWP
                     EPRVIVRFWIISLMLVLIGLATLKVR"
     gene            12821..14137
                     /gene="murD"
                     /locus_tag="NBI74_001713"
     CDS             12821..14137
                     /gene="murD"
                     /locus_tag="NBI74_001713"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HCD1932071.1"
                     /translation="MADYQGKNVVIIGLGLTGLSCVDFFLARGVTPRVMDTRMTPPGL
                     DKLPEAVERHTGGLNDEWLMAADLIVASPGIALAHPSLSAAADAGIEIVGDIELFCRE
                     AQAPIVAITGSNGKSTVTTLVGEMAKAAGVNVGVGGNIGLPALMLLDDECELYVLELS
                     SFQLETTSSLQAVAATILNVTEDHMDRYPFGLQQYRAAKLRIYENAKVCVVNADDALT
                     MPIRGADERCVSFGVNMGDYHLNHQQGETWLRVKGEKVLNVKEMKLSGQHNYTNALAA
                     LALADAAGLPRASSLKALTTFTGLPHRFEVVLEHNGVRWVNDSKATNVGSTEAALNGL
                     HVDGTLHLLLGGDGKSADFSPLARYLNGDNVRLYCFGRDGAQLAALRPEVAEQTETME
                     QAMRLLAPRVQPGDMVLLSPACASLDQFKNFEQRGNEFARLAKELG"
     gene            14137..15381
                     /gene="ftsW"
                     /locus_tag="NBI74_001714"
     CDS             14137..15381
                     /gene="ftsW"
                     /locus_tag="NBI74_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsW"
                     /protein_id="HCD1932072.1"
                     /translation="MRLSLPRLKMPRLPGFSILVWISTALKGWVMGSREKDTDSLIMY
                     DRTLLWLTFGLAAIGFIMVTSASMPIGQRLTNDPFFFAKRDGVYLILAFILAIITLRL
                     PMEFWQRYSATMLLGSIILLMIVLVVGSSVKGASRWIDLGLLRIQPAELTKLSLFCYI
                     ANYLVRKGDEVRNNLRGFLKPMGVILVLAVLLLAQPDLGTVVVLFVTTLAMLFLAGAK
                     LWQFIAIIGMGISAVVLLILAEPYRIRRVTAFWNPWEDPFGSGYQLTQSLMAFGRGEL
                     WGQGLGNSVQKLEYLPEAHTDFIFAIIGEELGYVGVVLALLMVFFVAFRAMSIGRKAL
                     EIDHRFSGFLACSIGIWFSFQALVNVGAAAGMLPTKGLTLPLISYGGSSLLIMSTAIM
                     MLLRIDYETRLEKAQAFVRGSR"
     gene            15378..16445
                     /gene="murG"
                     /locus_tag="NBI74_001715"
     CDS             15378..16445
                     /gene="murG"
                     /locus_tag="NBI74_001715"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HCD1932073.1"
                     /translation="MSGQGKRLMVMAGGTGGHVFPGLAVAHHLMAQGWQVRWLGTADR
                     MEADLVPKHGIEIDFIRISGLRGKGIKALIAAPLRIFNAWRQARAIMKAYKPDVVLGM
                     GGYVSGPGGLAAWSLGIPVVLHEQNGIAGLTNKWLAKIATKVMQAFPGAFPNAEVVGN
                     PVRTDVLALPLPQQRLAGREGPVRVLVVGGSQGARILNQTMPQVAAKLGDSVTIWHQS
                     GKGSQQSVEQAYAEVGQPQHKVTEFIDDMAAAYAWADVVVCRSGALTVSEIAAAGLPA
                     LFVPFQHKDRQQYWNALPLEKAGAVKIIEQSQLSVDAVANTLAGWSRETLLTMAERAR
                     AASIPDATERVANEVSRAARA"
     gene            16499..17974
                     /gene="murC"
                     /locus_tag="NBI74_001716"
     CDS             16499..17974
                     /gene="murC"
                     /locus_tag="NBI74_001716"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HCD1932074.1"
                     /translation="MNTQQLAKLRSIVPEMRRVRHIHFVGIGGAGMGGIAEVLANEGY
                     QISGSDLAPNPVTQQLMNLGATIYFNHRPENVRDASVVVVSSAISADNPEIVAAHEAR
                     IPVIRRAEMLAELMRFRHGIAIAGTHGKTTTTAMVSSIYAEAGLDPTFVNGGLVKAAG
                     VHARLGHGRYLIAEADESDASFLHLQPMVAIVTNIEADHMDTYQGDFENLKQTFINFL
                     HNLPFYGRAVMCVDDPVIRELLPRVGRQTTTYGFSEDADVRVEDYQQIGPQGHFTLLR
                     QDKEPMRVTLNAPGRHNALNAAAAVAVATEEGIDDEAILRALESFQGTGRRFDFLGEF
                     PLEPVNGKSGTAMLVDDYGHHPTEVDATIKAARAGWPDKNLVMLFQPHRFTRTRDLYD
                     DFANVLTQVDTLLMLEVYPAGEAPIPGADSRSLCRTIRGRGKIDPILVPDPAQVAEML
                     APVLTGNDLILVQGAGNIGKIARSLAEIKLKPQTPEEEQHD"
     gene            17967..18887
                     /gene="ddlB"
                     /locus_tag="NBI74_001717"
     CDS             17967..18887
                     /gene="ddlB"
                     /locus_tag="NBI74_001717"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HCD1932075.1"
                     /translation="MTDKIAVLLGGTSAEREVSLNSGAAVLAGLREGGIDAYPVDPKE
                     VDVTQLKSMGFQKVFIALHGRGGEDGTLQGMLELMGLPYTGSGVMASALSMDKLRSKL
                     LWQSAGLPVAPWVALTRAEFEKGLNDKQLAEISSLGLPVIVKPSREGSSVGMSKVVAE
                     NALQDALRLAFQHDEEVLIEKWLSGPEFTVAILGEEILPSIRIQPAGTFYDYEAKYLS
                     DETQYFCPAGLEASQEANLQALVLKAWTTLGCKGWGRIDVMLDSDGQFYLLEANTSPG
                     MTSHSLVPMAARQAGMSFSQLVVRILELAD"
     gene            18889..19719
                     /gene="ftsQ"
                     /locus_tag="NBI74_001718"
     CDS             18889..19719
                     /gene="ftsQ"
                     /locus_tag="NBI74_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ"
                     /protein_id="HCD1932076.1"
                     /translation="MSQAALNTRNSEEEVSSRRNNGTRLAGILFLLTVLTTVLVSGWV
                     VLGWMEDAQRLPLSKLVLTGERHYTRNDDIRQSILALGEPGTFMTQDVNIIQTQIEQR
                     LPWIKQVSVRKQWPDELKIHLVEYVPIARWNDQHMVDAEGNTFSVPPDRTSKQVLPML
                     YGPEGSANEVLQGYREMGQMLAKDRFTLKEAAMTARRSWQLTLNNDIKLNLGRGDTMK
                     RLARFVELYPVLQQQAQTDGKRISYVDLRYDSGAAVGWAPLPPEESTQQQNQAQAEQQ
                     "
     gene            19716..20978
                     /gene="ftsA"
                     /locus_tag="NBI74_001719"
     CDS             19716..20978
                     /gene="ftsA"
                     /locus_tag="NBI74_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HCD1932077.1"
                     /translation="MIKATDRKLVVGLEIGTAKVAALVGEVLPDGMVNIIGVGSCPSR
                     GMDKGGVNDLESVVKCVQRAIDQAELMADCQISSVYLALSGKHISCQNEIGMVPISEE
                     EVTQEDVENVVHTAKSVRVRDEHRVLHVIPQEYAIDYQEGIKNPVGLSGVRMQAKVHL
                     ITCHNDMAKNIVKAVERCGLKVDQLIFAGLASSYSVLTEDERELGVCVVDIGGGTMDI
                     AVYTGGALRHTKVIPYAGNVVTSDIAYAFGTPPSDAEAIKVRHGCALGSIVGKDESVE
                     VPSVGGRPPRSLQRQTLAEVIEPRYTELLNLVNEEILQLQEKLRQQGVKHHLAAGIVL
                     TGGAAQIEGLAACAQRVFHTQVRIGAPLNITGLTDYAQEPYYSTAVGLLHYGKESHLN
                     GEAEVEKRVTASVGSWIKRLNSWLRKEF"
     gene            21039..22190
                     /gene="ftsZ"
                     /locus_tag="NBI74_001720"
     CDS             21039..22190
                     /gene="ftsZ"
                     /locus_tag="NBI74_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HCD1932078.1"
                     /translation="MFEPMELTNDAVIKVIGVGGGGGNAVEHMVRERIEGVEFFAVNT
                     DAQALRKTAVGQTIQIGSGITKGLGAGANPEVGRNAADEDRDALRAALEGADMVFIAA
                     GMGGGTGTGAAPVVAEVAKDLGILTVAVVTKPFNFEGKKRMAFAEQGITELSKHVDSL
                     ITIPNDKLLKVLGRGISLLDAFGAANDVLKGAVQGIAELITRPGLMNVDFADVRTVMS
                     EMGYAMMGSGVASGEDRAEEAAEMAISSPLLEDIDLSGARGVLVNITAGFDLRLDEFE
                     TVGNTIRAFASDNATVVIGTSLDPDMNDELRVTVVATGIGMDKRPEITLVTNKQVQQP
                     VMDRYQQHGMAPLTQEQKPVAKVVNDNAPQTAKEPDYLDIPAFLRKQAD"
     gene            22291..23208
                     /gene="lpxC"
                     /locus_tag="NBI74_001721"
     CDS             22291..23208
                     /gene="lpxC"
                     /locus_tag="NBI74_001721"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HCD1932079.1"
                     /translation="MIKQRTLKRIVQATGVGLHTGKKVTLTLRPAPANTGVIYRRTDL
                     NPPVDFPADAKSVRDTMLCTCLVNEHDVRISTVEHLNAALAGLGIDNIVIEVNAPEIP
                     IMDGSAAPFVYLLLDAGIDELNCAKKFVRIKETVRVEDGDKWAEFKPYNGFSLDFTID
                     FNHPAIDSSNQRYAMNFSADAFMRQISRARTFGFMRDIEYLQSRGLCLGGSFDCAIVV
                     DDYRVLNEDGLRFEDEFVRHKMLDAIGDLFMCGHNIIGAFTAYKSGHALNNKLLQAVL
                     AKQEAWEYVTFQDDAELPLAFKAPSAVLA"
     gene            23438..23950
                     /gene="secM"
                     /locus_tag="NBI74_001722"
     CDS             23438..23950
                     /gene="secM"
                     /locus_tag="NBI74_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308128.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secA regulator SecM"
                     /protein_id="HCD1932080.1"
                     /translation="MSGILTRWRQFGKRYFWPHLLLGMVAASLGLPALSNAAEPNAPA
                     KATTRNHEPSAKVNFGQLALLETNTRRPNSNYSVDYWHQHAIRTVIRHLSFAMAPQTL
                     PVAEESLPLQAQHLALLDTLSALLTQEGTPSEKGYRIDYAHFTPQAKFSTPVWISQAQ
                     GIRAGPQRLS"
     gene            24012..26717
                     /gene="secA"
                     /locus_tag="NBI74_001723"
     CDS             24012..26717
                     /gene="secA"
                     /locus_tag="NBI74_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HCD1932081.1"
                     /translation="MLIKLLTKVFGSRNDRTLRRMRKVVNIINAMEPEIEKLSDEELK
                     GKTAEFRARLEKGEVLENLIPEAFAVVREASKRVFGMRHFDVQLLGGMVLNERCIAEM
                     RTGEGKTLTATLPAYLNALTGKGVHVVTVNDYLAQRDAENNRPLFEFLGLTVGINLPG
                     MPAPAKREAYAADITYGTNNEYGFDYLRDNMAFSPEERVQRKLHYALVDEVDSILIDE
                     ARTPLIISGPAEDSSEMYKRVNKIIPHLIRQEKEDSETFQGEGHFSVDEKSRQVNLTE
                     RGLVLIEELLVKEGIMDEGESLYSPANIMLMHHVTAALRAHALFTRDVDYIVKDGEVI
                     IVDEHTGRTMQGRRWSDGLHQAVEAKEGVQIQNENQTLASITFQNYFRLYEKLAGMTG
                     TADTEAFEFSSIYKLDTVVVPTNRPMIRKDLPDLVYMTEAEKIQAIIEDIKERTAKGQ
                     PVLVGTISIEKSELVSNELTKAGIKHNVLNAKFHANEAAIVAQAGYPAAVTIATNMAG
                     RGTDIVLGGSWQAEVAALENPTAEQIEKIKADWQVRHDAVLAAGGLHIIGTERHESRR
                     IDNQLRGRSGRQGDAGSSRFYLSMEDALMRIFASDRVSGMMRKLGMKPGEAIEHPWVT
                     KAIANAQRKVESRNFDIRKQLLEYDDVANDQRRAIYSQRNELLDVSDVSETINSIRED
                     VFKATIDAYIPPQSLEEMWDIPGLQERLKNDFDLDLPIAEWLDKEPELHEETLRERIL
                     AQSIEVYQRKEEVVGAEMMRHFEKGVMLQTLDSLWKEHLAAMDYLRQGIHLRGYAQKD
                     PKQEYKRESFSMFAAMLESLKYEVISTLSKVQVRMPEEVEELEQQRRMEAERLAQMQQ
                     LSHQDDDSAAAAALAAQTGERKVGRNDPCPCGSGKKYKQCHGRLQ"
     gene            26777..27175
                     /gene="mutT"
                     /locus_tag="NBI74_001724"
     CDS             26777..27175
                     /gene="mutT"
                     /locus_tag="NBI74_001724"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase MutT"
                     /protein_id="HCD1932082.1"
                     /translation="MKKLQIAVGIIRNENNEIFITRRAADAHMANKLEFSGGKIEMGE
                     TPEQAVVRELQEEVGITPQHFSLFEKLEYEFPDRHITLWFWLVESWEGEPWGKEGQPG
                     EWMSLVGLNADDFPPANEPVIAKLKRVYVE"
     gene            complement(27263..27460)
                     /gene="yacG"
                     /locus_tag="NBI74_001725"
     CDS             complement(27263..27460)
                     /gene="yacG"
                     /locus_tag="NBI74_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HCD1932083.1"
                     /translation="MSETITVNCPTCGKTVVWGEISPFRPFCSKRCQLIDLGEWAAEE
                     KRIPSSGDLSESDDWSEEPKQ"
     gene            complement(27470..28213)
                     /gene="zapD"
                     /locus_tag="NBI74_001726"
     CDS             complement(27470..28213)
                     /gene="zapD"
                     /locus_tag="NBI74_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapD"
                     /protein_id="HCD1932084.1"
                     /translation="MQTQVLFEHPLNEKMRTWLRIEFLIQQLTVNLPIVDHAGALHFF
                     RNVSELLDVFERGEVRTELLKELDRQQRKLQTWIGVPGVDQSRIEALIQQLKAAGSVL
                     ISAPRIGQFLREDRLIALVRQRLSIPGGCCSFDLPTLHIWLHLPQAQRDSQVETWIAS
                     LNPLTQALTMVLDLIRQSAPFRKQTSLNGFYQDNGGDADLLRLNLSLDSQLYPQISGH
                     KSRFAIRFMPLDSENGQVPERLDFELACC"
     gene            complement(28213..28833)
                     /gene="coaE"
                     /locus_tag="NBI74_001727"
     CDS             complement(28213..28833)
                     /gene="coaE"
                     /locus_tag="NBI74_001727"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HCD1932085.1"
                     /translation="MRYIVALTGGIGSGKSTVANAFADLGINVIDADIIARQVVEPGA
                     PALHAIADHFGANMIAADGTLQRRALRERIFANPEEKNWLNALLHPLIQQETQHQIQQ
                     ATSPYVLWVVPLLVENSLYKKANRVLVVDVSPETQLKRTMQRDDVTREHVEQILAAQA
                     TREARLAVADDVIDNNGAPDAIASDVARLHAHYLQLASQFVSQEKP"
     gene            28858..28902
                     /locus_tag="NBI74_001728"
     CDS             28858..28902
                     /locus_tag="NBI74_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1932086.1"
                     /translation="MIIKTLKMSARKRN"
     gene            29058..30101
                     /gene="guaC"
                     /locus_tag="NBI74_001729"
     CDS             29058..30101
                     /gene="guaC"
                     /locus_tag="NBI74_001729"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008461816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HCD1932087.1"
                     /translation="MRIEEDLKLGFKDVLIRPKRSTLKSRSDVELERQFTFKHSGQRW
                     SGVPIIAANMDTVGTFSMASALASFDILTAVHKHYSVEEWQAFINNSSADVLKHVMVS
                     TGTSDADFEKTKQILDLNPALNFVCIDVANGYSEHFVQFVAKAREAWPTKTICAGNVV
                     TGEMCEELILSGADIVKVGIGPGSVCTTRVKTGVGYPQLSAVIECADAAHGLGGMIVS
                     DGGCTTPGDVAKAFGGGADFVMLGGMLAGHEESGGRIVEENGEKFMLFYGMSSESAMK
                     RHVGGVAEYRAAEGKTVKLPLRGPVENTARDILGGLRSACTYVGASRLKELTKRTTFI
                     RVQEQENRIFNNL"
     gene            complement(30136..31338)
                     /gene="hofC"
                     /locus_tag="NBI74_001730"
     CDS             complement(30136..31338)
                     /gene="hofC"
                     /locus_tag="NBI74_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein transport protein HofC"
                     /protein_id="HCD1932088.1"
                     /translation="MASKQLWRWRGITGDGNAQDGMLWAESRALLLMALQQQMVTPLS
                     LKRIAINSAQWRGDKSAEVIHQLATLLKAGLTLSEGLALLAEQHPSKQWQALLQSLAH
                     DLEQGVAFSNALLPWSEVFPPLYQAMIRTGELTGKLDECCFELARQQKAQRQLTDKVK
                     SALRYPIIILAMAIMVVVAMLHFVLPEFAAIYKTFNTPLPALTQGIMTLASFSGEWGW
                     LLVLFGFLLAIANKLLMRHPTRLIARQKLLLRIPIMGSLMRGQKLTQIFTILTLTQSA
                     GISFLQGVESVRETMRCPYWVQLLTQIQHDISNGHPIWLALKNAGEFSPLCLQLVRTG
                     EASGSLDLMLDNLAHHHRDNTMALADNLAALLEPALLIITGGIIGTLVVAMYLPIFHL
                     GDAMSGMG"
     gene            complement(31328..32713)
                     /gene="gspE"
                     /locus_tag="NBI74_001731"
     CDS             complement(31328..32713)
                     /gene="gspE"
                     /locus_tag="NBI74_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspE"
                     /protein_id="HCD1932089.1"
                     /translation="MNIPQLTALCLRYQGVLLDASEEVVHVAVVDAPSHELLDALHFA
                     TTKRIEITCWTRQQMEGHASRTQQTLPVAVQEKHQPKAELLTRTLQSALEQRASDIHI
                     EPADNAYRIRLRIDGVLHPLPDVSPDAGVALTARLKVLGNLDIAEHRLPQDGQFTVEL
                     AGNAVSFRIATLACRGGEKVVLRLLQQVNQALDVNTLGMQPSQLVDFAHALQQPQGLV
                     LVTGPTGSGKTVTLYSALQTLNTADINICSVEDPVEIPIAGLNQTQIHSRAGLTFQGV
                     LRALLRQDPDVIMIGEIRDGETAEIAIKAAQTGHLVLSTLHTNSTCETLVRLQQMGVA
                     RWMLSSALTLVIAQRLVRKLCPHCRRQQGEPIHIPDNVWPSPLPHWQAPGCVHCYHGF
                     YGRTALFEVLPITPVIRQLISANTDVESLETHARQAGMRTLFENGCLAVEQGLTTFEE
                     LIRVLGMPHGE"
     gene            complement(32723..33163)
                     /gene="ppdD"
                     /locus_tag="NBI74_001732"
     CDS             complement(32723..33163)
                     /gene="ppdD"
                     /locus_tag="NBI74_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent pilin"
                     /protein_id="HCD1932090.1"
                     /translation="MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQ
                     TFVPYRTAVELCALEHGGLNTCDGGSNGIPSPTTTRYVSAMSVAKGVVSLTGQESLNG
                     LSVVMTPGWDNANGVTGWTRNCNIQSDSALQQACEDVFRFDDAN"
     gene            complement(33367..34260)
                     /gene="nadC"
                     /locus_tag="NBI74_001733"
     CDS             complement(33367..34260)
                     /gene="nadC"
                     /locus_tag="NBI74_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HCD1932091.1"
                     /translation="MPPRRYNPDTRRDELLERINLDIPGAVAQALREDLGGTVDANND
                     ITAKLLPENSRSHATVITRENGVFCGKRWVEEVFIQLAGDDVTIIWHVDDGDVINANQ
                     PLFELEGPSRVLLTGERTALNFVQTLSGVASKVRHYVELLEGTNTQLLDTRKTLPGLR
                     SALKYAVLCGGGANHRLGLSDAFLIKENHIIASGSVRQAVEKASWLHPDAPVEVEVEN
                     LEELDEALKAGADIIMLDNFETEQMREAVKRTNGKALLEVSGNVTDKTLREFAETGVD
                     FISVGALTKHVQALDLSMRFR"
     gene            34348..34899
                     /gene="ampD"
                     /locus_tag="NBI74_001734"
     CDS             34348..34899
                     /gene="ampD"
                     /locus_tag="NBI74_001734"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,6-anhydro-N-acetylmuramyl-L-alanine amidase
                     AmpD"
                     /protein_id="HCD1932092.1"
                     /translation="MLLEQGWLAGARRVPSPHYDCRPDDETPTLLVVHNISLPPGEFG
                     GPWIDALFTGTIDPQAHPFFAEIAHLRVSAHCLIRRDGEIVQYVPFDKRAWHAGVSQY
                     QGRERCNDFSIGIELEGTDTLAYTDAQYQQLAAVTRALIDRYPDIANNMTGHCDIAPD
                     RKTDPGPAFDWARFRALVSKETT"
     gene            34896..35750
                     /gene="ampE"
                     /locus_tag="NBI74_001735"
     CDS             34896..35750
                     /gene="ampE"
                     /locus_tag="NBI74_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase regulator AmpE"
                     /protein_id="HCD1932093.1"
                     /translation="MTLFTTLLVLIFERLFKLGEHWQLDHRLEAFFRRVKHFSLGRTL
                     CMTIIAMGVTFLLLRALQGVLFNVPTLLVWLLIGLLCIGAGKVRLHYHAYLTAASRND
                     SHAHATMAGELTMIHGVPAGCDEREYLRELQNALLWINFRFYLAPLFWLIVGGTWGPV
                     TLMGYAFLRAWQYWLARYQTPHHRLQSGIDAVLHALDWVPVRLAGVVYALIGHGEKAL
                     PAWFASLGDFHTSQYQVLTRLAQFSLAREPHVDKVETPKAAVSMAKKTSFVVVVVIAL
                     LTIYGALV"
     gene            complement(35793..37163)
                     /gene="aroP"
                     /locus_tag="NBI74_001736"
     CDS             complement(35793..37163)
                     /gene="aroP"
                     /locus_tag="NBI74_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transporter AroP"
                     /protein_id="HCD1932094.1"
                     /translation="MEGQQHGEQLKRGLKNRHIQLIALGGAIGTGLFLGSASVIQSAG
                     PGIILGYAIAGFIAFLIMRQLGEMVVEEPVAGSFSHFAYKYWGSFAGFASGWNYWVLY
                     VLVAMAELTAVGKYIQFWYPEIPTWVSAAVFFVVINAINLTNVKVFGEMEFWFAIIKV
                     IAVVAMIIFGAWLLFSGNGGPQASVSNLWDQGGFLPHGFTGLVMMMAIIMFSFGGLEL
                     VGITAAEADNPEQSIPKATNQVIYRILIFYIGSLAVLLSLMPWTRVTADTSPFVLIFH
                     ELGDTFVANALNIVVLTAALSVYNSCVYCNSRMLFGLAQQGNAPKALASVDKRGVPVN
                     TILVSALVTALCVLINYLAPESAFGLLMALVVSALVINWAMISLAHMKFRRAKQEQGV
                     VTRFPALLYPLGNWICLLFMAAVLVIMLMTPGMAISVYLIPVWLIVLGIGYLFKEKTA
                     KAVKAH"
BASE COUNT         8853 a         9109 c        10431 g         9088 t
ORIGIN      
        1 gctttttctt attttatatg catgataaat catattctcg aggattattt ctctgcattc
       61 caataaggga aagggagtta agtgtgacag tggagttaag tatgccagag gtacaaacag
      121 atcatccaga gacggcggag ttaagcaagc cacagctacg catggtcgat ctcaacttat
      181 taaccgtttt cgatgccgtg atgcaggagc aaaacatcac ccgtgccgct catgctctgg
      241 gaatgtcgca acctgcggtc agtaacgctg ttgcacgcct gaaggtgatg tttaatgacg
      301 agctttttgt tcgttatggt cgtggtattc aaccgactgc tcgcgcattt caactttttg
      361 gctcagttcg tcaggcattg caattagtac aaaatgaatt accaggttca ggctttgagc
      421 ctgccagtag tgaacgtgta tttcatcttt gtgtctgcag cccgttagac agcattctga
      481 cctcgcagat ttataatcac attgagcaga ttgcgccaaa tatacatgtt atgttcaagt
      541 cttcattaaa tcagaacact gaacatcagc tgcgttatca ggaaacggag tttgtgatta
      601 gttatgaaga cttccatcgc cctgaattta ccagtgtgcc attatttaaa gatgaaatgg
      661 tgttggtagc cagcaaaaat catccaacaa ttaaaggccc gttactgaag catgatgttt
      721 ataacgaaca acatgcggcg gtttcgctcg atcgtttcgc atcatttagt caaacctggt
      781 atgacacggt agataagcaa gccagtatcg cgtatcaggg catggcaatg atgagcgtac
      841 ttagcgtggt gtcgcaaacg catctggttg ctattgcgcc gcgttggttg gctgaagagt
      901 tcgctgaatc cttagagtta caggtattac cgctgccgtt aaaacaaaac agcagaacct
      961 gttatctctc ctggcatgaa gctgccgggc gcgataaagg ccatcagtgg atggaagagc
     1021 aattagtctc aatttgcaaa cgctaactga atgcagaata ggtcagacat caatgtctgg
     1081 tttattctgc attttttatt gaatgtagaa ttttattctg aatatttgtg ctctctattt
     1141 taggattaat taaaaaaata gagaaattgc tgtaagttgt aggattcagc cgatttatta
     1201 tcaatttcat cctctgtaat ggaggatttt atcgtttctt ttcacctttc ctcctgttta
     1261 ttcttattac cccgtgttta tgtctctgtc tgccaattgc ttaagcaaga tcggacggtt
     1321 aatgtgtttt acacattttt tccgtcaaac agtgaggcag gccatggaga tgttgtctgg
     1381 agccgagatg gtcgtccgat cgcttatcga tcagggtgtt aaacaagtat tcggttatcc
     1441 tggaggcgcg gtccttgata tttacgatgc attgcatacc gtgggtggta tcgatcatgt
     1501 tttagttcgc catgagcagg cggcggtgca tatggccgat ggtctggcgc gcgcgaccgg
     1561 ggaagtcggc gtcgtgctgg taacgtcggg tccaggggcg accaatgcga ttactggcat
     1621 tgccaccgct tatatggatt ccattccatt agttgtcctt tccgggcagg tagcgacctc
     1681 gttgataggt tatgatgcct ttcaggagtg cgacatggtg gggatttctc gcccggtggt
     1741 taaacacagt tttctggtta agcaaacgga agacattccg caggtgctga aaaaggcttt
     1801 ctggctagcg gcaagtggtc gtcctggacc cgtggtcgtt gatttaccga aagatattct
     1861 aaatccggcg aacaaattac cctatgtctg gccggagtcg gtcagtatgc gttcttacaa
     1921 tcccacgacc tccgggcata aagggcaaat taagcgcgct ctgcaaacgc tggtggcggc
     1981 aaaaaaaccg gttgtctacg taggcggtgg ggcaatcacg gcgggctgcc atctgcagtt
     2041 gaaagaagcg gtggaggtgt tgaatctgcc cgttgtttcc tctttgatgg ggctgggggc
     2101 gtttccggca acgcatcgcc aggcactggg catgttggga atgcacggta cctacgaagc
     2161 caatatgacg atgcataacg cggatgtgat tttcgccgtc ggggtacgtt ttgatgaccg
     2221 aacgacgaac aatctggcaa agtactgccc aaatgccact gttctgcata tcgatattga
     2281 tccaacctca atttctaaaa ccgtgactgc tgatatcccg attgtggggg atgctcgcca
     2341 ggtcctagaa caaatgcttg aactcctgtc gcaagaatcc gcccatcaac cgctggatga
     2401 gatccgcgac tggtggcagc agattgaaca gtggcgcgct cgtcagtgcc tgaaatatga
     2461 cactcacagt gaaaagatta aaccgcaggc ggtgatcgag actctttggc ggttgactaa
     2521 tggggacgct tacgtgacgt ccgatgtcgg gcagcaccag atgtttgctg cgctttatta
     2581 tccattcgac aaaccgcgtc gctggatcaa ctctggcggc ctcggcacga tgggttttgg
     2641 tttacctgcg gcactgggcg tcaaaatggc gttgccagaa gaaaccgtgg tttgcgtcac
     2701 tggtgacggc agtattcaga tgaacatcca ggaactgtct accgccttgc aatacgagtt
     2761 gcccgtactg gtggtgaatc tcaataatcg ctatctgggg atggtgaagc agtggcagga
     2821 catgatctat tccggccgtc attcacaatc ttatatgcag tccttaccag acttcgtccg
     2881 tctggcggaa gcctatggtc acgtcgggat ccagatctct catccgcaag aactggaaag
     2941 caaacttagc gaggcgctgg aacaggtgcg caataatcgc ctggtgtttg ttgatgttac
     3001 cgtcgatggc agtgagcacg tctacccgat gcagattcgc gggggcggaa tggatgaaat
     3061 gtggttaagc aaaacggaga gaacctgatt atgcgccgga tattatcagt cttactcgaa
     3121 aatgaatcag gcgcgttatc ccgcgtgatt ggcctttttt cccagcgtgg ctacaacatt
     3181 gaaagcctga ccgttgcgcc aaccgacgat ccgacattat cgcgtatgac catccagacc
     3241 gtgggcgatg aaaaagtgct tgagcaaatc gaaaagcaat tacacaagct ggtcgatgtc
     3301 ttgcgcgtga gcgagttggg gcagggcgcg cacgttgagc gggaaatcat gctggtgaaa
     3361 attcaggcca gcggttacgg gcgtgacgaa gtgaaacgta atacggaaat attccgtggg
     3421 caaattatcg atgtcacacc ctcgctttat accgttcaat tagcaggcac cagcgataag
     3481 cttgatgcat ttttagcatc gattcgcgat gtggcgaaaa ttgtggaagt tgctcgctct
     3541 ggtgtggtcg gactttcgcg cggcgataaa ataatgcgtt gagaatgatc tcaatgcgca
     3601 atttgcagcc caacatgtca cgttgggctt tttttgcgaa atcagtggga acctggaata
     3661 aaagcagttg ccgcagttaa ttttctgcgc ttagatgtta atgaatttaa cccataccag
     3721 tacaatggct atggttttta cattttacgc aaggggcaat tgtgaaactg gatgaaatcg
     3781 ctcggctggc gggagtgtcg cggaccactg caagctatgt tattaacggc aaagcgaagc
     3841 aataccgtgt gagcgacaaa accgttgaaa aagtcatggc tgtggtgcgt gagcacaatt
     3901 accacccgaa cgccgtggca gctgggcttc gtgctggacg cacacgttct attggtcttg
     3961 tgatccccga tctggagaac accagctata cccgcatcgc taactatctt gaacgccagg
     4021 cgcggcaacg gggttatcaa ctgctgattg cctgctcgga agatcagccc gacaacgaaa
     4081 tgcgctgtat tgagcacctt ttacagcgtc aggttgatgc cattattgtt tcgacgtcgt
     4141 tgccacctga gcatcctttt tatcaacgct gggctaacga cccgttcccg attgtcgcgc
     4201 tggaccgcgc cctcgatcgt gaacacttca ccagcgtggt tggtgccgat caggatgatg
     4261 ccgaaatgct ggcggaagag ttacgtaagt ttcccgccga gacggtgctt tatcttggcg
     4321 cgctgccgga gctttctgtc agcttcctgc gtgaacaagg cttccgtact gcctggaaag
     4381 atgatccgcg cgaagtgcat ttcctttatg ccaacagcta tgagcgggag gcagctgccc
     4441 agttattcga aaaatggctg gaaacgcatc cgatgccgca ggcgctgttc acaacgtcgt
     4501 ttgcgttgtt gcaaggggtg atggatgtca cgctacgtcg cgacggcaaa ctgccttctg
     4561 acctggcaat tgccaccttt ggcgataacg aactgctcga cttcttacag tgcccggtgc
     4621 tggcagtggc tcaacgtcac cgcgatgtcg cagaacgtgt gctggagatt gtcctggcga
     4681 gcctggacga accgcgtaag ccaaaacctg gtttaacgcg cattaaacgt aatctctatc
     4741 gccgcggcgt gctcagccgt agctaaaccg cgaacaaaag tacgcgccag gtgaatttct
     4801 ctctggcgcg ttgagtacgg gaatggacat caatatcctt aaagtaaata agactattcc
     4861 tggctattat tgataaatgc ttttaaaccc gcccgttaat taactcacct gctgaaatgc
     4921 acaataatta aatgatattg acagcgcgtt tttgcattat tttgttacat gcagagatga
     4981 attgccgatt taacaaacac ttttctttgt ttttgcgcaa acctgctggc atcaagcgcc
     5041 acacagacgc aacagggact gttaaccaga gaagatatgt cctaaaatgc cgctcgcgtc
     5101 gcaaactgac actttatatt tgctgtggaa aatagtgagt cattttaaaa cggtgatgac
     5161 gatgagggat tttttcttac agctattcat aacgttaatt tgcttcgcac gttggacgta
     5221 aaataaacaa cgctgatatt agccgtaaac atcgggtttt ttacctcggt atgccttgtg
     5281 actggcttga caagcttttc ctccgctccg taaactcctt ttagtgggaa attgtggggc
     5341 aaagtgggaa taaggggtga ggctggcatg ttccggggag caacgttagt caatctcgac
     5401 agcaaagggc gcttatcagt gcctacccgt tatcgggaac agctgcttga gaacgctgcc
     5461 ggtcaaatgg tttgcaccat tgacattcat cacccgtgcc tgctgcttta ccccctgcct
     5521 gaatgggaaa ttatcgagca aaaattatcg cgtctgtcga gcatgaaccc ggttgagcgc
     5581 cgtgtgcagc gcctactgtt aggtcatgcc agcgaatgtc agatggatgg cgcaggtcga
     5641 ttgttaatcg cgccagtact gcggcaacat gccgggctga caaaagaagt gatgctggtt
     5701 ggacagttca acaagtttga gctgtgggat gaaacaacct ggcatcaaca ggtcaaggaa
     5761 gatatcgacg cagagcagtt ggctaccgga gacttatcgg agcgactgca ggacttgtct
     5821 ctataaaatg atggaaaact ataaacatac tacggtgctg ctggatgaag ccgttaatgg
     5881 cctcaatatc cgtcctgatg gcatctacat tgatgggact tttggtcgcg gtggtcactc
     5941 acgtctgatc ctctcgcagc ttggcgaaga ggggcgtttg ctggcgatcg atcgcgaccc
     6001 gcaggctatc gccgttgcga agactattga tgatccgcgc ttctccatca tccacggacc
     6061 tttctctgcg ctgggcgaat atgttgccga gcgcgatctt atcggcaaga tcgacggcat
     6121 tctcctcgat cttggcgtct cttcaccgca acttgatgat gctgaacgcg gcttttcctt
     6181 tatgcgcgat ggtccgctgg acatgcgtat ggacccaacc cgtgggcagt cagccgctga
     6241 atggctacaa accgcagaag aagccgatat cgcctgggta ttgaaaacct atggtgaaga
     6301 gcgttttgcc aaacgcattg ctcgcgccat tgtcgagcgt aaccgcgaac agccgatgac
     6361 ccgcaccaaa gaactggcgg aagtcgtggc tgccgcaacg ccggtgaaag ataagtttaa
     6421 acatcccgcg acccgtacct tccaggcggt gcgcatttgg gtaaacagtg aactggagga
     6481 gatagagcag gcgctaaaaa gctcgctcaa tgtgctggcc ccgggggggc ggctttcgat
     6541 catcagcttc cactcacttg aagaccgcat tgtgaaacgt tttatgcgtg aaaacagccg
     6601 cggtccgcaa gttccggcag ggttaccgat gacagaagag cagctcaaaa aactgggggg
     6661 gcgtcagttg cgagcactag gtaagttgat gccgggcgaa gaagaggtgg ctgagaaccc
     6721 tcgtgcccgt agttcagttc tgcgtattgc agagaggacg aacgcatgat cagcagagtg
     6781 acagaagctc taagcaaagt taaaggatcg atgggaagcc acgagcgcca tgccttgcct
     6841 ggcgttatcg gtgacgatct tttgcgattt gggaagctgc cactctgcct gttcatttgc
     6901 attattttga cggcggtgac cgtggtaacc acggcgcacc atacccgttt actgaccgct
     6961 cagcgcgaac aactggtgct ggagcgagat gctttagaca ttgaatggcg caacctgatc
     7021 cttgaagaga atgcgctcgg cgaccatagc cgggtggaaa ggatagccac ggaaaagctg
     7081 caaatgcagc atgttgatcc gtcacaagaa aatatcgtag tgcaaaaata aggataaacg
     7141 cgacgcatga aagcagcggc gaaaacgcag aaaccaaaac gtcaggaaga acatgccaac
     7201 tttatcagtt ggcgttttgc gttgttatgc ggctgtattc tcctggcgct ggcttttctg
     7261 ctcggacgcg tagcgtggtt acaagttatc tccccggata tgctggtgaa agagggcgac
     7321 atgcgttctc ttcgcgttca gcaagtttcc acctcccgcg gcatgattac tgaccgttct
     7381 ggtcgcccgt tagcggtgag tgtgccagta aaagcgattt gggctgaccc gaaagaagtg
     7441 catgacgctg gcggtatcag cgtcggtgac cgctggaagg cgctggctaa cgcgctcaat
     7501 attccgctgg atcagctctc agcccgcatt aacgccaacc cgaaagggcg ctttatttat
     7561 ctggcgcgtc aggtaaaccc tgacatggcg gactacatca aaaaactgaa actgccggga
     7621 attcatctgc gtgaagaatc tcgccgttat tatccatccg gcgaagtgac tgctcacctc
     7681 atcggcttta ctaacgtcga tagccaaggg attgaggggg tcgagaagag tttcgataaa
     7741 tggcttaccg ggcagccggg tgagcgcatt gtgcgtaaag accgctatgg tcgcgtaatt
     7801 gaagatattt cttctactga cagccaggca gcgcacaacc tgacgctgag tattgatgaa
     7861 cgcctgcagg cgctggttta tcgcgaactg aacaacgcag tggcctttaa caaggctgaa
     7921 tctggtagcg ccgtgctggt ggatgtcaac accggtgaag tgctggcgat ggctaacagc
     7981 ccgtcataca acccaaataa tctgagcggc acgccgaaag aagcgatgcg taaccgtacc
     8041 atcaccgacg tgtttgaacc gggctcaacg gttaaaccga tggtggtgat gaccgcgttg
     8101 caacgtggcg tggtgcggga aaactcggta ctcaataccg ttccttatcg aattaacggc
     8161 cacgaaatca aagacgtggc acgctacagc gaattaaccc tgaccggggt attacagaag
     8221 tcgagtaacg tcggtgtttc caaactggcg ttagcgatgc cgtcctcagc gttagtagat
     8281 acttactcac gttttgggct gggaaaagcg accaatttgg ggttggtcgg agaacgcagt
     8341 ggcttatatc ctcaaaaaca acggtggtct gacatagaga gggccacctt ctctttcggc
     8401 tacgggctaa tggtaacacc gttacagtta gcgcgagtct acgcaactat cggcagctac
     8461 ggcatttatc gcccactgtc gattaccaaa gttgaccccc cggttcccgg tgaacgtgtc
     8521 ttcccggaat ccattgttcg caccgtggtg catatgatgg aaagcgtggc gctaccgggc
     8581 ggcggcggcg tgaaggcggc gattaaaggc tatcgtatcg ccattaaaac cggtaccgcg
     8641 aaaaaggtcg ggccggatgg tcgctacatc aataaatata ttgcttatac cgcaggcgtt
     8701 gcgcctgcga gtcagccgcg cttcgcgctg gttgttgtta tcaacgatcc gcaggcgggt
     8761 aaatactacg gcggcgccgt ttccgcgccg gtctttggtg ccatcatggg cggcgtattg
     8821 cgtaccatga acatcgagcc ggatgcgctg acaacaggcg ataaaaatga atttgtgatt
     8881 aatcaaggcg agggtacagg tggcagatcg taatttgcgc gaccttcttg ctccgtgggt
     8941 gccagacgca ccttcgcgag cactgcgaga gatgacactc gacagccgtg tggctgcggc
     9001 gggcgatctc tttgtagctg tagtaggtca tcaggcggac gggcgtcgat atatcccgca
     9061 ggcgatagcg caaggtgtgg ctgccattat tgcagaggcg aaagatgagg cgaccgacgg
     9121 tgaaatccgt gaaatgcacg gcgtaccggt catctatctc agccagctca acgagcgttt
     9181 atctgcactg gcgggccgct tttaccatga accctctgac aatttacgtc tcgtgggcgt
     9241 aacgggcacc aacggcaaaa ccacgactac ccagctgttg gcgcagtgga gtcaactgct
     9301 tggcgaaacc agcgcggtaa tgggcaccgt tggtaacggc ctgctgggga aagtgatccc
     9361 gacagaaaat acaaccggtt cggcagtcga tgttcagcat gagctggcgg ggctggtgga
     9421 tcagggcgcg actttttgcg caatggaagt ttcatcccac gggctggttc aacaccgtgt
     9481 ggcggcattg aaatttgcgg cgtcggtctt taccaactta agccgcgatc accttgatta
     9541 tcatggtgat atggaacact acgaagccgc gaaatggctg ctttattctg cacatcattg
     9601 cggtcaggcg attattaacg ccgacgatga agtgggccgc cgctggctgg caaaactgcc
     9661 ggacgcggtt gcggtatcaa tggaagatca tatcaatccg aactgtcacg gacgctggtt
     9721 gaaagcgacc gacgtgaact atcacgacag cggtgcgacc atttgcttta gctcgagttg
     9781 gggcgatggg gaaattgaaa gtcgcctgat gggcgcattc aacgtcagca acctgctgct
     9841 ggcgctggcg acactgctgg cactcggcta tccgctggct gatttgctga aaaccgccgc
     9901 gcgtctgcaa ccggtttgcg gacgtatgga agtgttcact gcaccaggca aaccgacggt
     9961 ggtggtggat tacgcgcata cgccggatgc actggaaaaa gccttacagg cggcgcgcct
    10021 gcactgtgcg ggcaagctgt ggtgcgtctt tggctgcggt ggcgatcgtg ataaaggcaa
    10081 acgtccactg atgggcgcaa ttgctgaaga gtttgctgac gtggtggtgg tgacggacga
    10141 taacccgcgt accgaagagc ctcgtgccat catcaacgat attctggcgg gaatgttaga
    10201 tgccggatat gccaaagtga tggaaggccg tgctgaagcg gtgacttgcg ccgttatgca
    10261 ggctaaagag aatgatgtgg tactggtcgc gggcaaaggc catgaggact accagattgt
    10321 tggcaatcag cgtctggact actccgatcg cgtcacggtg gcgcgtctgc tgggggtgat
    10381 tgcatgatta gcgtaaccct tagccaactt accgatattc tcaacggtga actgcaaggt
    10441 gcagatatta cccttgatgc tgtaaccact gacacgcgaa aactgacgcc gggctgcctg
    10501 tttgttgccc tgaaaggcga acgtttcgat gctcatgatt ttgccgacca ggcgaaagct
    10561 ggcggcgcag gcgcactact ggttagccgt ccgctggata ttgatctgcc gcagttaatc
    10621 gtcaaggata cgcgtctggc gtttggtgaa ctggctgcat gggttcgcca gcaagttccg
    10681 gcgcgcgtgg ttgctctgac aggttcctcc ggcaaaacct ccgttaaaga gatgacggcg
    10741 gcaattttaa gccagtgcgg caacacactt tatacggcag gcaatctcaa taacgacatc
    10801 ggtgtaccga tgacgctgtt gcgcttaacg ccggaatacg attacgcagt tattgaactt
    10861 ggcgcgaacc atcagggtga aattgcctgg actgtgagtc tgactcgccc ggaagcggcg
    10921 ctggtcaaca acctggcagc ggcgcatctg gaaggttttg gctcgcttgc gggtgtcgcg
    10981 aaagcgaaag gtgaaatctt tagcggcctg ccggaaaacg gtatcgccat tatgaacgcc
    11041 gacaacaacg actggttgaa ctggcagagc gtaattggct cacgcaaagt gtggcgtttc
    11101 tcacctaatg ccgccaacag cgatttcacc gccaccaata tccatgtaac ctcgcacggt
    11161 acggaattta ccctgcaaac ccctacaggt agcgtcgatg ttctgctgcc gttgccgggg
    11221 cgtcacaata ttgcgaatgc gctggcagcc gctgcgctct ccatgtctgt gggcgcaacg
    11281 cttgatgcta tcaaagcggg gctggtaaat ctgaaagctg ttccaggccg tctgttcccc
    11341 ataaaactgg cagaaaacca gttgctgctc gacgactcct acaacgccaa tgtcggttca
    11401 atgaccgccg ctgttcaggt tctggctgaa atgccgggct accgcgtgct ggtggtgggc
    11461 gatatggcgg aactgggcgc tgaaagcgaa gcctgccatg tacaggtggg cgaagcggca
    11521 aaagctgctg gtattgactg cgtgttaagt gtgggtaaac aaagccatgc tatcagcacc
    11581 gccagcggcg ttggcgaaca tttttccgat aaaactgcgc ttatcgcgcg tcttaaatca
    11641 ctgattgctg agcaacaggt aattacgatt ttagttaagg gttcacgtag tgctgccatg
    11701 gaagaggtag tacgcgcttt acaggagaat gggacatgtt agtttggctg gccgaacatt
    11761 tggtcaaata ttattccggc tttaacgtct tttcctatct gacgtttcgc gccatcgtca
    11821 gcctgctgac cgcgctgttc atctcattgt ggatgggccc gcgtatgatt gctcatttgc
    11881 aaaaactttc ctttggtcag gtggtgcgta acgacggtcc tgaatcacac ttcagcaagc
    11941 gcggtacacc gaccatgggc gggataatga tcctgacggc gattgtgatc tccgtgctgc
    12001 tgtgggctta cccgtccaat ccgtacgtct ggtgcgtact ggtggtgctg gtaggttacg
    12061 gtgttattgg ctttgttgat gattatcgca aagtggtgcg taaagacacc aagggcctga
    12121 ttgcccgttg gaagtatttc tggatgtcgg tcattgcact gggtgttgcc ttcgcgctgt
    12181 accttgccgg caaagacacg cccgcaacgc agttggtggt tccgttcttt aaagatgtga
    12241 tgccgcaact ggggctgttc tacattctgc tggcttactt tgtcattgtg ggtactggca
    12301 acgcggtaaa cctgaccgat ggtctcgacg gtctggcaat tatgccgacc gtatttgtcg
    12361 ccggtggttt tgcgctggtg gcgtgggcga ccggcaacat gaactttgcc agctatttac
    12421 acataccgta tctacgacac gccggggaac tggtcattgt ctgtaccgcg atagtcgggg
    12481 cagggctggg cttcctgtgg tttaacacct atccggcgca ggtctttatg ggcgatgtag
    12541 gttcgctggc gttaggtggt gcgttaggta ttatcgccgt actgctgcgt caggaattcc
    12601 tgctggtgat tatggggggc gtgttcgtgg tagaaacgct ttccgtcatc ctgcaggtcg
    12661 gctcctttaa actgcgcgga caacgtattt tccgcatggc accgattcat caccactatg
    12721 aactgaaagg ctggccggaa ccgcgcgtca ttgtgcgttt ctggattatt tcgctgatgc
    12781 tggtcctgat tggtctggca acgctgaagg tacgttaatc atggctgatt accagggtaa
    12841 aaatgtcgtc attatcggcc tgggcctcac cgggctttcc tgcgtggact ttttcctcgc
    12901 tcgcggtgtg acgccgcgcg ttatggatac gcgtatgaca ccgcctggcc tggataaatt
    12961 acccgaagcc gtagaacgcc acacgggcgg tctgaatgat gaatggctga tggcggcaga
    13021 tctgattgtc gccagtcccg gtattgcact ggcgcatcca tccttaagcg ctgccgctga
    13081 tgccggaatc gaaatcgttg gcgatatcga gctgttctgt cgtgaagcac aagcaccgat
    13141 tgtggcgatc accggttcta acggcaaaag cacggtcacc acgctagtgg gtgaaatggc
    13201 gaaagcggcg ggggttaacg ttggtgtggg tggtaatatt ggcctgcctg cgttgatgct
    13261 gttggatgat gagtgcgaac tgtacgtgct ggaactgtcg agtttccagc tggaaaccac
    13321 ttccagctta caagcggtgg cggcgaccat tctgaacgtg actgaagatc atatggatcg
    13381 ctatccgttt ggtttacaac agtatcgtgc agcaaaactg cgcatttacg aaaacgcaaa
    13441 agtttgcgtg gttaatgctg atgatgcctt aacaatgccg attcgcggtg cggatgaacg
    13501 ctgcgtcagc tttggcgtca acatgggcga ctatcacctg aatcatcagc agggcgaaac
    13561 ctggctgcgg gtgaaaggcg agaaagtgct gaacgtgaaa gaaatgaaac tttccgggca
    13621 gcataactac accaatgcgc tggcggcgct ggcgctggca gatgccgcag ggttaccgcg
    13681 tgccagcagc ctgaaagcgt taaccacatt cactggtctg ccgcatcgct ttgaagttgt
    13741 gctggagcat aacggtgtgc gctgggttaa cgattcaaaa gcgaccaacg tcggcagtac
    13801 ggaagcggcg ctgaatggcc tgcatgtaga cggcacgctg catttgttgc tgggtggcga
    13861 tggtaaatcg gcggacttta gcccactggc gcgttacctg aatggcgata atgtacgtct
    13921 gtattgtttc ggtcgggacg gcgcgcagct ggcggcgcta cgcccggaag tggcagaaca
    13981 aaccgaaacc atggaacagg cgatgcgctt gcttgctccg cgtgttcagc cgggcgatat
    14041 ggttctgctc tccccggcct gtgccagcct tgatcaattc aagaactttg aacaacgagg
    14101 caatgagttt gcccgtctgg cgaaggagtt aggttgatgc gtttatctct ccctcgcctg
    14161 aaaatgccgc gcctgccagg attcagtatt ctggtctgga tctccacggc gctaaagggc
    14221 tgggtgatgg gctcgcggga aaaagatacc gacagcctga tcatgtacga tcgcacctta
    14281 ctgtggctga ccttcggcct cgcggcgatt ggctttatca tggtgacctc ggcgtcaatg
    14341 cccatagggc aacgcttaac caacgatccg ttcttcttcg cgaagcgtga tggcgtctat
    14401 ctgatcctgg cattcattct ggcgatcatt acgctgcgtc tgccgatgga gttctggcaa
    14461 cgctacagtg ccacgatgct gcttggatct atcatcctgc tgatgatcgt tctggtagtg
    14521 ggtagctcgg ttaaaggggc atcgcgttgg atcgatctcg gtttgctgcg tatccagcct
    14581 gcggagctga caaaactgtc gctgttttgc tatatcgcca actatctggt gcgtaaaggc
    14641 gacgaagtac gtaataacct gcgcggcttt ctgaaaccga tgggcgtaat tctggtgttg
    14701 gctgtgttac tgctggcaca gccagacctt ggtacggtgg tggtgttgtt tgtgactacg
    14761 ctggcgatgt tgttcctggc gggagcgaaa ttgtggcagt tcattgccat tatcggtatg
    14821 ggcatttcag cggttgtgtt gctgatcctc gccgaaccgt accgtatccg ccgtgttacc
    14881 gcattctgga atccgtggga agatcccttt ggcagcggct atcagttaac gcaatcgttg
    14941 atggcgtttg gtcgcggtga actgtgggga caaggtttag gtaactcggt acaaaaactg
    15001 gagtatctgc cggaagcgca cactgacttt attttcgcca ttatcggcga agaactggga
    15061 tatgtcggtg tggtgctggc acttttaatg gtattcttcg tcgcttttcg cgcgatgtcg
    15121 attggccgca aagcattaga aattgaccac cgtttttccg gttttctcgc ctgttctatt
    15181 ggtatctggt ttagttttca ggcgctggtt aacgtaggcg cggcggcggg gatgttaccg
    15241 accaaaggtc tgacattgcc gctgatcagt tacggtggtt cgagcttact gattatgtcg
    15301 acagccatca tgatgctgtt gcgtattgat tatgaaacgc gtctggagaa agcgcaggcg
    15361 tttgtacgag gttcacgatg agtggtcaag gaaagcgatt aatggtgatg gcaggcggaa
    15421 ccggtggaca tgtattcccg gggctggcgg ttgcgcacca tctaatggct cagggttggc
    15481 aagttcgctg gctggggact gccgaccgta tggaagcgga cttagtgcca aaacatggca
    15541 tcgaaattga tttcattcgt atctctggtc tgcgtggaaa aggtataaaa gcactgatag
    15601 ctgcgccgct gcgtatcttc aacgcctggc gtcaggcgcg ggcgattatg aaagcgtaca
    15661 aacctgacgt ggtgctcggt atgggcggct atgtatcagg tccaggtggt ctggccgcgt
    15721 ggtcgttagg cattccggtt gtacttcatg aacaaaacgg tattgcgggc ttaaccaata
    15781 aatggctggc gaagattgct accaaagtga tgcaggcgtt tccaggtgct ttccctaatg
    15841 cggaagtggt gggtaacccg gtgcgtaccg atgtgctggc gctgccgttg ccgcagcaac
    15901 gtttggctgg acgtgaaggt ccggttcgtg tgttggtagt gggtggttcc cagggcgcac
    15961 gcattcttaa tcagacaatg ccgcaggttg ctgcgaaact gggtgattca gtcactatct
    16021 ggcatcagag cggcaaaggt tcgcaacaat ccgttgaaca ggcgtatgcc gaagtggggc
    16081 aaccgcagca taaagtgacg gaatttattg atgatatggc ggcggcgtat gcgtgggcgg
    16141 atgtcgtcgt ttgccgctcc ggtgcgttaa cggtgagtga aatcgccgcg gcaggactac
    16201 cggcgttgtt tgtgccgttt caacataaag accgtcagca atactggaat gcgctaccgc
    16261 tggaaaaagc gggcgcagtc aaaattatcg agcagtcaca gcttagcgtg gatgctgtcg
    16321 ccaacaccct ggccgggtgg tcgcgagaaa ccttattaac catggcagaa cgcgcccgcg
    16381 ctgcatccat tccggatgcc accgagcgag tggcaaatga agtgagccgg gctgcccggg
    16441 cgtaattgta gcgatgcctt ttgcatcgta tgaatttaag aagttaatgg cgtaaagaat
    16501 gaatacacaa caattggcaa aactgcgttc catcgtgccc gaaatgcgtc gcgttcggca
    16561 catacatttt gtcggcatcg gtggtgccgg tatgggcggt attgccgaag ttctggccaa
    16621 tgaaggttat cagatcagtg gttccgattt agcgccaaat ccggtcacgc agcagttaat
    16681 gaatctggga gcgacgattt atttcaacca tcgcccggaa aacgtacgtg atgccagcgt
    16741 ggtcgttgtt tccagcgcga tttctgccga taacccggaa attgtcgcag ctcatgaagc
    16801 gcgtattccg gtgatccgtc gtgccgaaat gctggctgag ttaatgcgtt ttcgtcatgg
    16861 catcgccatt gccggaacgc acggcaaaac gacaaccacc gcgatggttt ccagcatcta
    16921 cgcagaagcg gggctcgacc caaccttcgt taacggcggg ctggtaaaag cggcgggggt
    16981 tcatgcgcgt ttggggcatg gtcggtacct gattgccgaa gcagatgaga gtgatgcatc
    17041 gttcctgcat ctgcaaccga tggtggcgat tgtcaccaat atcgaagccg accacatgga
    17101 tacctaccag ggcgactttg agaatttaaa acagactttt attaattttc tgcacaacct
    17161 gccgttttac ggtcgcgcgg tgatgtgtgt tgatgatccg gtgatccgcg aattgttacc
    17221 gcgtgtgggg cgtcagacca cgacttacgg cttcagcgaa gatgccgacg tgcgtgtaga
    17281 agattatcag cagattggcc cgcaggggca ctttacgctg ctgcgccagg acaaagagcc
    17341 gatgcgcgtc accctgaatg cgccaggtcg tcataacgcg ctgaacgccg cagctgcggt
    17401 tgcggttgct acggaagagg gaattgacga cgaggctatt ttgcgtgcgc tggagagctt
    17461 ccaggggacg gggcgccgtt tcgatttcct cggtgaattc ccgctggagc cagtgaatgg
    17521 taaaagcggt acggcaatgc tggtcgatga ctacggccac cacccgacgg aagtggacgc
    17581 cactattaaa gcggcgcgtg caggctggcc ggataaaaac ctggtaatgc tgtttcagcc
    17641 gcaccgtttt acccgtacgc gcgacctgta tgatgatttc gccaatgtgc tgacgcaggt
    17701 tgataccctg ttgatgctgg aagtgtatcc ggctggcgaa gcgccaattc cgggagcgga
    17761 cagccgttcg ctgtgtcgca caattcgtgg acgtgggaaa attgatccca ttctggtgcc
    17821 ggacccggcg caggtagccg agatgctggc accggtatta accggtaacg acctgattct
    17881 cgttcagggg gctggtaata tcggaaaaat tgctcgttct ttagctgaaa tcaaactgaa
    17941 gccgcaaact ccggaggaag aacaacatga ctgataaaat cgcggtcctg ttgggcggga
    18001 cctccgctga gcgggaagtt tctctgaatt ctggcgcagc ggtgttagcc gggctgcgtg
    18061 aaggcggtat tgacgcgtat cctgtcgacc cgaaagaagt cgacgtgacg caactgaagt
    18121 cgatgggttt tcagaaagtg tttatcgcgc tacacggtcg cggcggtgaa gatggtacgc
    18181 tgcagggcat gctcgagctg atgggcttgc cttataccgg cagcggagtg atggcatctg
    18241 cgctttcaat ggataaacta cgcagcaaac ttctgtggca gagtgccggt ttaccggtcg
    18301 cgccgtgggt agcattaacc cgcgcagagt ttgaaaaagg ccttaacgat aagcagttag
    18361 cagaaatttc ttctctgggt ttgccggtaa tcgttaagcc gagccgcgaa ggttccagtg
    18421 tgggaatgtc aaaagtagta gcagaaaatg ctctacaaga tgcattaaga ttggcatttc
    18481 agcacgatga agaagtattg attgaaaaat ggctaagtgg gccggagttc acggttgcga
    18541 tactcggtga agaaatttta ccgtcaatac gtattcaacc ggccggaacc ttctatgatt
    18601 atgaggcgaa gtatctctct gatgagacac agtatttctg ccccgcaggt ctggaagcgt
    18661 cacaagaggc caatttgcag gcattagtgc taaaagcatg gacgacgtta ggttgcaaag
    18721 gatggggacg tattgacgtt atgctggaca gcgatggaca gttttatctg ctggaagcca
    18781 atacctcacc gggtatgacc agccacagtc tggtgccgat ggcggcacgt caggcaggta
    18841 tgagcttctc gcagttggta gtacgaattc tggaactggc ggactaatat gtcgcaggct
    18901 gctctgaaca cgcgaaatag cgaagaagag gtttcttctc gccgcaataa tggaacgcgt
    18961 ctggcgggga tccttttcct gctgaccgtt ttaacgacag tgttggtgag cggctgggtc
    19021 gtgttgggct ggatggaaga tgcgcaacgc ctgccgctct caaagctggt gttgaccggt
    19081 gaacgccatt acacgcgtaa tgacgatatc cggcagtcga tcctggcatt gggtgagcca
    19141 ggcaccttta tgacccagga tgtcaacatc atccagacgc aaatagaaca acgcctgccg
    19201 tggattaagc aggtgagcgt cagaaagcag tggcctgatg aattgaagat tcatctggtt
    19261 gaatatgtgc cgattgcgcg gtggaatgat caacatatgg tagacgcgga aggaaacacc
    19321 ttcagcgtgc cgccagatcg caccagcaag caggtgcttc caatgctgta tggcccggaa
    19381 ggcagcgcca atgaagtgtt gcagggctat cgcgaaatgg ggcagatgct ggcaaaggac
    19441 agatttactc tgaaggaagc ggcgatgacc gcgcgacgtt cctggcagtt gacgctgaat
    19501 aacgatatta agctcaatct tggccggggc gatacgatga aacgtttggc tcgctttgta
    19561 gaactttatc cggttttaca gcagcaggcg caaaccgatg gcaaacggat tagctacgtt
    19621 gatttgcgtt atgactctgg agcggcagta ggctgggcgc ccttgccgcc agaggaatct
    19681 actcagcaac aaaatcaggc acaggcagaa caacaatgat caaggcgacg gacagaaaac
    19741 tggtagtagg actggagatt ggtaccgcga aggttgccgc tttagtaggg gaagttctgc
    19801 ccgacggtat ggtcaatatc attggcgtgg gtagttgccc gtcgcgtggt atggataaag
    19861 gcggggtgaa cgacctcgaa tccgtggtca agtgcgtaca acgcgccatt gaccaggcag
    19921 aattgatggc agattgtcag atatcttcgg tatatctggc gctttctggt aagcacatca
    19981 gctgccagaa tgaaattggt atggtgccta tttctgaaga agaagtgacg caagaagatg
    20041 tggaaaacgt cgtccatacc gcgaaatcgg tgcgtgtgcg cgatgagcat cgtgtgctgc
    20101 atgtgatccc gcaagagtat gcgattgact atcaggaagg gatcaagaat ccggtaggac
    20161 tttcgggcgt gcggatgcag gcaaaagtgc acctgatcac atgtcacaat gatatggcga
    20221 aaaacatcgt caaagcggtt gaacgttgtg ggctgaaagt tgaccaactg atatttgccg
    20281 gtctggcatc aagttattcg gtattgacgg aagatgaacg tgaactgggt gtctgcgtcg
    20341 tcgatatcgg tggtggtaca atggatatcg ccgtttatac cggtggggca ttgcgccaca
    20401 cgaaggtaat tccttatgct ggcaatgtcg tgaccagtga tatcgcttac gcctttggca
    20461 caccgccaag tgacgccgaa gcgattaaag ttcgccacgg ttgtgcgctg ggctccatcg
    20521 ttggaaaaga tgagagcgtg gaagtgccga gcgtaggcgg tcgtccgccg cggagtctgc
    20581 aacgtcagac cctggcagag gtgatcgagc cacgctatac cgagctgctc aatctggtca
    20641 acgaagagat attgcagttg caggaaaagc ttcgccaaca aggggttaaa catcacctgg
    20701 cggcaggcat tgtattaacc ggtggcgcag cgcagatcga aggtcttgca gcctgtgctc
    20761 agcgcgtgtt tcatacgcaa gtgcgtatcg gcgcgccgct gaacattacc ggtttaacgg
    20821 attatgctca ggagccgtat tattcgacgg cggtgggatt gcttcactat gggaaagagt
    20881 cacatcttaa cggtgaagct gaagtagaaa aacgtgttac agcatcagtt ggctcgtgga
    20941 tcaagcgact caatagttgg ctgcgaaaag agttttaatt tttatgaggc cgacgatgat
    21001 tacggcctca ggcgacaggc acaaatcgga gagaaactat gtttgaacca atggaactta
    21061 ccaatgacgc ggtgattaaa gtcatcggcg tcggcggcgg cggcggtaat gctgttgaac
    21121 acatggtgcg tgagcgcatt gaaggtgttg aattcttcgc ggtaaatacc gatgcacaag
    21181 cgctgcgtaa aacagcggtt ggacagacga ttcaaatcgg tagcggtatc accaaaggac
    21241 tgggcgctgg cgctaatcca gaagttggcc gcaatgcggc tgatgaggat cgcgatgcac
    21301 tgcgtgcggc gctggaaggt gcagacatgg tctttattgc tgcgggtatg ggtggtggca
    21361 ccggtacagg tgcagcacca gtcgtcgctg aagtggcaaa agatttgggt atcctgaccg
    21421 ttgctgtcgt cactaagcct ttcaactttg aaggcaagaa gcgtatggca ttcgcggagc
    21481 aggggatcac tgaactgtcc aagcatgtgg actctctgat cactatcccg aacgacaaac
    21541 tgctgaaagt tctgggccgt ggtatctccc tgctggatgc gtttggcgca gcgaacgatg
    21601 tactgaaagg cgctgtgcaa ggtatcgccg aactgattac tcgtccgggt ttgatgaacg
    21661 tggactttgc agacgtacgc accgtaatgt ctgaaatggg ctacgcaatg atgggttctg
    21721 gtgtggcgag cggtgaagac cgtgcggaag aagctgctga aatggctatc tcttctccgc
    21781 tgctggaaga tatcgacctg tctggcgcac gcggcgtgct ggtaaacatc acagcgggct
    21841 tcgacctgcg tctggatgag ttcgaaacgg taggtaacac catccgtgca tttgcttccg
    21901 acaacgcgac cgtggttatc ggtacttctc ttgacccgga tatgaatgac gagctgcgcg
    21961 taactgttgt tgcgacaggt atcggcatgg acaaacgtcc tgaaatcact ctggtgacca
    22021 ataagcaggt tcagcagcca gtgatggatc gctaccagca gcacggtatg gctccgctga
    22081 cccaggagca gaagccggtt gctaaagtcg tgaatgacaa tgcgccgcaa actgcgaaag
    22141 agccggatta tctggatatc ccagcattcc tgcgtaagca agctgattaa gaattgactg
    22201 gaatttgggt ttcgaggctc tttgtgctaa actggcccgc cgaatgtata gtacacttcg
    22261 gttggatagg taatttggcg agataatacg atgatcaaac aaaggacact taaacgtatc
    22321 gttcaggcga cgggtgtcgg tttacatacc ggcaagaaag tcaccctgac gttacgccct
    22381 gcgccggcca acaccggggt catctatcgt cgcaccgact tgaatccacc ggtagatttc
    22441 ccggccgatg ccaaatctgt gcgtgatacc atgctctgta cgtgtctggt caacgagcat
    22501 gatgtacgga tttcaaccgt agagcacctc aatgctgctc tcgctggctt gggcatcgat
    22561 aacattgtta tcgaagttaa cgcgccggaa atcccgatca tggacggcag cgccgctccg
    22621 tttgtatacc tgctgcttga cgccggtatc gacgagttga actgcgccaa gaaatttgtt
    22681 cgcattaaag agactgttcg tgtcgaagat ggcgataagt gggctgaatt taaaccgtac
    22741 aatggttttt cgctggattt caccatcgat tttaaccatc cggctattga ttccagcaac
    22801 cagcgctatg cgatgaactt ctccgctgat gcgtttatgc gccagatcag ccgtgcgcgt
    22861 acgttcggtt tcatgcgtga tatcgaatat ctgcagtccc gtggtttgtg cctgggcggc
    22921 agcttcgatt gtgccatcgt tgttgacgat tatcgcgtac tgaacgaaga cggcctgcgt
    22981 tttgaagacg aatttgtgcg tcacaaaatg ctcgatgcga tcggtgactt gttcatgtgt
    23041 ggtcacaata ttattggtgc atttaccgct tataaatccg gtcatgcact gaataacaaa
    23101 ctgctgcagg ctgtcctggc gaaacaggaa gcctgggaat atgtgacctt ccaggacgac
    23161 gcagaactgc cgttggcctt caaagcgcct tcagccgtac tggcataacg acatttatac
    23221 tgtcgtataa aattcgactg gcaaatctgg cactctctcc ggccaggtga accagtcgtt
    23281 ttttttgaat tttataagag ctataaaaaa cggtgcgaac gctgttttct taagcacttt
    23341 tacgcacaac ttatcttcat tcgtgctgtg gacttcaggc tttaatgata agatttgtgc
    23401 gctaaatacg tttgaatacg atcgggatgg caataacgtg agtggaatac tgacgcgctg
    23461 gcgacagttt ggtaaacgct acttctggcc gcatctctta ttagggatgg tcgcggcgag
    23521 tttaggtttg cctgcactca gcaatgccgc cgaaccaaac gcgcccgcaa aagcgacaac
    23581 ccgcaaccac gagccttcag ccaaagttaa ctttggtcaa ttggccttgc tggaaacgaa
    23641 cacgcgccgc ccgaattcga actattccgt tgattactgg catcaacatg ccattcgcac
    23701 ggtaatccgc catctttcct ttgcaatggc accgcaaacg ctgcccgttg ctgaagaatc
    23761 tttgcctctt caggcgcaac atcttgcatt actggatacg ctcagcgcgc tgctgaccca
    23821 ggaaggcacg ccgtctgaaa agggttatcg cattgattat gcgcatttta ccccacaagc
    23881 aaaattcagc acgcccgtct ggataagcca ggcgcaaggc atccgtgctg gccctcaacg
    23941 cctcagctaa caacaataaa cctttacttc attttattaa ctccgcaacg cggggcgttt
    24001 gagattttat tatgctaatc aaattattaa ctaaagtttt cggtagtcgt aacgatcgca
    24061 ccctgcgccg gatgcgcaaa gtggtcaaca tcatcaatgc catggaaccg gagattgaaa
    24121 aactctccga cgaagaactg aaaggaaaaa cggcagagtt ccgcgcgcgt ctggaaaaag
    24181 gcgaagtgct ggaaaacttg atcccggaag ccttcgccgt ggtgcgtgaa gccagtaagc
    24241 gtgtttttgg catgcgccac ttcgacgtac agctgctcgg cggtatggtt cttaatgaac
    24301 gctgcatcgc ggaaatgcgt accggtgaag gtaaaaccct gaccgcaacg ctgcctgctt
    24361 acctgaacgc actaaccggt aaaggcgtgc acgtcgttac cgtcaacgac tacctggcgc
    24421 aacgtgacgc cgaaaacaac cgtccgctgt ttgaattcct tggcctgact gtcggtatca
    24481 acctgccggg catgccagca ccggcaaagc gtgaagccta cgctgctgac atcacttacg
    24541 gtacgaacaa cgaatacggc tttgactacc tgcgtgacaa catggcgttc agtcctgaag
    24601 aacgtgtaca gcgtaaactg cactatgcgc tggtggacga agtggactcc atcctgatcg
    24661 atgaagcgcg tacaccgctg atcatttctg gcccggcaga agacagctcg gaaatgtata
    24721 aacgcgtgaa taaaattatt ccgcacctga tccgtcagga aaaagaagac tccgaaacct
    24781 tccagggcga aggccacttc tcggtggacg aaaaatctcg ccaggtgaac ctgaccgaac
    24841 gtggtctggt gctgattgaa gaactgctgg tgaaagaagg catcatggat gaaggggagt
    24901 ctctgtactc tccggccaac atcatgctga tgcaccacgt aacggcggcg ctgcgcgctc
    24961 atgcgctgtt tacccgtgac gtcgactaca tcgttaaaga tggtgaagtt atcatcgttg
    25021 acgaacacac tggtcgtacc atgcaggggc gtcgctggtc tgatggtctg caccaggctg
    25081 tggaagcgaa agaaggtgtg cagatccaga acgaaaacca aacgctggct tcgatcacct
    25141 tccagaacta cttccgtctg tatgaaaaac tggcggggat gactggtact gctgataccg
    25201 aagctttcga attcagctcc atctataagc tggatactgt cgttgttccg accaaccgtc
    25261 caatgattcg taaagatctg ccggacctgg tctacatgac tgaagcggaa aaaattcagg
    25321 cgatcattga agatatcaaa gaacgtactg cgaaaggcca gccggtgctg gtgggtacaa
    25381 tctccatcga aaaatcggag ctggtgtcaa atgaactgac caaagccggt attaagcaca
    25441 acgtcctgaa cgccaaattc catgccaacg aagcggcgat tgttgctcag gcaggttatc
    25501 cggctgcggt gactatcgcg accaacatgg cgggtcgtgg taccgatatt gtgctcggtg
    25561 gtagctggca ggcagaagtt gccgcgctgg aaaatccgac tgcagagcaa attgaaaaaa
    25621 ttaaagccga ctggcaggta cgtcacgatg cggtactggc agcaggtggc ctgcatatca
    25681 tcggtactga acgtcacgaa tcccgtcgta tcgataacca gctgcgcggt cgttctggtc
    25741 gtcaggggga tgctggttct tcccgtttct atctgtcgat ggaagatgcg ctgatgcgta
    25801 tttttgcctc cgaccgagta tccggcatga tgcgtaaact aggtatgaag ccaggcgaag
    25861 ccattgagca cccgtgggtg accaaagcga ttgccaacgc ccagcgtaaa gttgaaagcc
    25921 gtaacttcga cattcgtaag caactgctgg aatatgatga cgtggctaac gatcagcgtc
    25981 gcgccattta ctcccagcgt aacgaactgc tggatgtcag cgatgtgagc gaaaccatca
    26041 acagcattcg tgaagatgtg ttcaaagcga ccattgatgc ctacattccg ccacagtcgc
    26101 tggaagaaat gtgggatatt ccggggctgc aggaacgtct gaagaacgat ttcgacctcg
    26161 atttgccaat tgccgagtgg ctggataaag aaccagaact gcatgaagag acgctgcgtg
    26221 agcgcattct ggcgcagtcc atcgaagtgt atcagcgtaa agaagaagtg gttggtgctg
    26281 agatgatgcg tcacttcgag aaaggcgtca tgctgcaaac gcttgactct ctgtggaaag
    26341 agcacctggc tgcgatggac tatctgcgtc agggtatcca cctgcgtggc tatgcacaga
    26401 aagatccgaa gcaggaatac aaacgtgaat cgttctccat gtttgcagcg atgctggagt
    26461 cgttaaaata tgaagttatc agcacgctga gcaaagttca ggtgcgtatg cctgaagagg
    26521 ttgaggagct ggaacaacag cgtcgtatgg aagccgagcg tttagcgcaa atgcagcagc
    26581 ttagccatca ggatgacgac tctgcagccg cagctgcact ggcggcgcaa accggtgaac
    26641 gaaaagtagg acgtaacgat ccttgcccgt gtggttctgg taaaaaatac aagcagtgcc
    26701 atggccgcct gcaataaaag ctaactgttg aagtaaaagg cgcaggattc tgcgcctttt
    26761 ttataggttt aagacaatga aaaagctgca aattgcggta ggtattattc gcaacgagaa
    26821 caatgaaatc tttataacgc gtcgcgcagc agatgcgcac atggcgaata aactggagtt
    26881 ttccggcggt aaaattgaaa tgggcgaaac gccggaacag gcagtggtac gtgaacttca
    26941 ggaagaagtc gggattaccc cacaacattt ttcgctattt gaaaaactgg aatatgaatt
    27001 cccggacagg catataactc tgtggttctg gctggtcgaa agctgggaag gggagccgtg
    27061 gggtaaagaa gggcaacccg gtgagtggat gtcgctggtc ggtcttaatg ccgatgattt
    27121 tccgccagcc aatgaaccgg taattgcgaa acttaagcgt gtgtatgtcg aataaggcgt
    27181 cacgctgcat ccgacatttg catatgatgc ctgatgcgac gctggcgcgt tttatcaggc
    27241 cagcatcgca tcagatagaa tgtcactgct ttggctcttc gctccagtca tcgctttcgg
    27301 aaagatcgcc actgctgggg attcgttttt cttcagcagc ccattctccg aggtcgatca
    27361 gctgacaacg tttggagcaa aatggccgaa acgggctgat ttcaccccac accaccgttt
    27421 tcccgcaggt tgggcaattc accgtaatag tttctgacat ttttactcct tagcaacagg
    27481 ccagttcgaa atccagacgt tctggtactt gtccgttttc actgtccagc ggcataaaac
    27541 gaatggcaaa acggctctta tgaccggaaa tttgcggata aagctgtgaa tcgagcgaca
    27601 gattcaggcg cagcaagtcg gcatcgccac cgttatcctg ataaaaacca ttcaggctgg
    27661 tttgtttacg gaagggggcc gactggcgaa ttaaatccag caccatggta agtgcctggg
    27721 taagtgggtt caggctggca atccaggttt ctacctggct gtcgcgctgc gcctggggta
    27781 gatgcagcca aatgtgcaat gtaggtaaat caaagctgca acagccgccc gggatgctca
    27841 gtcgctgacg caccagagca atcaaacgat cttcacgtag aaattgcccg atacgcggcg
    27901 cggaaattaa tacgctcccc gccgctttta actgctgaat taatgcttca atacggctct
    27961 ggtccacgcc gggcacgcca atccaggtct ggagtttacg ttgctgccgg tcaagttctt
    28021 tcaacagctc agtgcggact tcgccgcgct cgaaaacatc cagtaattcg ctgacattac
    28081 ggaagaaatg cagcgcgcca gcgtggtcaa cgatgggtaa attaacggtg agttgctgaa
    28141 tcaaaaactc aatgcgcagc catgtacgca ttttttcatt tagtggatgt tcaaaaagga
    28201 cctgggtctg cattacggtt tttcctgtga gacaaactgc gacgcaagct gcaaatagtg
    28261 tgcgtgcagg cgggcaacat ccgatgcgat agcatccggt gcgccgttat tatcaatgac
    28321 gtcatctgcc acggcaaggc gagcttcgcg cgttgcctga gcagcaagga tttgttcgac
    28381 atgctcgcga gttacatcgt cgcgctgcat ggtgcgctta agttgcgttt ctgggctgac
    28441 atccaccacc agcactcgat tcgctttttt atacagtgag ttttctacca gcaatggcac
    28501 aacccatagt acataggggg aagtagcttg ctggatctgg tgttgcgtct cttgctgaat
    28561 cagcggatgc agcagggcgt taagccagtt tttctcttcc gggttggcga agatccgctc
    28621 gcgcaaggcc cggcgctgca atgttccatc agcagcaatc atgttagcgc caaagtgatc
    28681 agcaatggca tgtagcgcag gtgcacctgg ttcaaccacc tgacgcgcaa taatatcggc
    28741 atcaatgacg ttaattccga gatcagcaaa cgcattggca acggtactct tgccactgcc
    28801 aatgcctccc gttaaggcaa ctatatacct cataatttat attcccggga attcatcatg
    28861 attatcaaaa cgttaaaaat gagcgcacga aagcgaaatt gatgaaacgt tcgctcacta
    28921 tttaccaggt aaatttatgg gattgtagcg taaaaaaaga caatttcgca gtcttgcgcc
    28981 gcattgatta gtgcgtatga taacgtcact ggagttgcgc tcttaccctt atagccatta
    29041 accccaggaa tccgcacatg cgtattgaag aagatctgaa gttaggtttt aaagacgttc
    29101 tcatccgccc taaacgctcc actcttaaaa gccgttccga tgttgaactg gaacgtcaat
    29161 tcaccttcaa acattcaggt cagcgctggt ccggcgtgcc aattatcgcc gccaatatgg
    29221 acaccgtagg cacattttct atggcctctg cgctggcttc ttttgatatt ttgactgctg
    29281 tgcataaaca ctattctgtc gaagagtggc aagcgtttat caacaattct tccgctgatg
    29341 tgctgaaaca tgtgatggtt tctaccggca cgtctgatgc ggatttcgaa aaaactaaac
    29401 agattctcga cctgaacccg gcattaaact tcgtttgtat tgacgtggcg aatggttatt
    29461 ccgaacactt cgtgcagttc gttgcgaaag cgcgtgaagc gtggccgacc aaaaccattt
    29521 gtgctggcaa cgtagtgact ggtgaaatgt gtgaggagct tatcctctcc ggtgctgata
    29581 tcgttaaagt tggcattggc ccaggttctg tttgtacaac tcgcgtcaaa acaggcgtcg
    29641 gttatccgca actttctgcg gtgatcgaat gtgccgatgc tgcgcatggt ctgggcggaa
    29701 tgatcgtcag cgacggtggc tgcaccacgc cgggcgatgt cgcgaaagcc tttggcggcg
    29761 gtgccgattt cgtcatgctt ggcggcatgc tggcgggcca cgaagagagc ggcggtcgca
    29821 tcgttgagga gaacggcgag aaatttatgc tgttctacgg catgagctca gagtctgcga
    29881 tgaaacgtca cgttggcggt gttgcggaat atcgcgcagc agaaggtaaa accgttaagc
    29941 tgccgctgcg aggcccggtt gaaaataccg cgcgtgatat tttgggcggc ctgcgttcag
    30001 cttgtacata cgttggggct tcacgcctga aagaactgac caagcgcacc acatttatcc
    30061 gtgtgcagga acaagaaaac cgcatcttca acaacctgta atataccaac gctggcgtgg
    30121 agcaacacgc cagcgttatc ccattccact catcgcatcg cctaaatgga aaattggcag
    30181 atacattgcc accaccagcg taccgataat tcctcccgtt atgatcagca acgccggttc
    30241 cagtaaggct gcgaggttat ccgccagcgc cattgtgtta tcccgatgat gatgggcgag
    30301 gttgtctaac atgagatcca gcgagccgga tgcctctcct gttctcacta attgcaaaca
    30361 gagcgggcta aactcaccgg cattttttag cgccagccag atggggtgac cgttactgat
    30421 atcgtgctgg atttgtgtca gaagttgcac ccagtacgga cagcgcattg tttctctgac
    30481 gctctcaacg ccctgtaaaa aagaaatgcc tgcactttgt gtcagcgtca gaatcgtaaa
    30541 gatctgcgtg agtttttgtc cccgcatcag tgaacccata atcgggatgc gtaacagtaa
    30601 tttctgccgt gcgataagcc tggttgggtg gcgcatcagc agcttattgg ctatcgccag
    30661 cagaaagccg aatagcacca gcaaccaacc ccattcgcca ctaaaacttg ccagcgtcat
    30721 gatcccctgc gttagcgccg gtagcggagt gttaaaggtc ttatagatag cggcaaactc
    30781 cggcagaaca aaatgcagca ttgccacaac caccatgatt gccatcgcta aaatgataat
    30841 gggataacgt aacgctgatt tcactttgtc ggtcaactga cgctgggctt tttgctgacg
    30901 cgccagttca aagcagcatt catccagctt accggtcagt tcacctgtgc ggatcatcgc
    30961 ctgatagagc ggcggaaata cctctgacca gggtaataag gcattggaaa aggcaacgcc
    31021 ctgttcaaga tcgtgcgcca gtgattgcag caacgcttgc cactgcttac tgggatgctg
    31081 ttccgccagc agagccagcc cttcagaaag cgttaacccg gctttgagta gcgtcgccag
    31141 ttgatgaatg acttccgcgc ttttatctcc tcgccactgc gcagaattga tggcgattcg
    31201 cttcaggctt agtggggtaa ccatctgttg ctgtagtgcc atgagcagca aagcacggct
    31261 ctctgcccat agcatcccat cttgcgcatt gccgtcgccg gttatgccac gccagcgcca
    31321 gagttgctta ctcgccatgc ggcatcccca atacgcggat taactcttca aaggtggtta
    31381 agccttgctc cacagccagg cagccgtttt caaaaagcgt tcgcataccc gcctgtcggg
    31441 cgtgcgtttc cagcgattca acgtcggtat tagcggaaat aagctgacgt atgaccggtg
    31501 ttatgggcag aacttcaaat aaggccgtgc gaccataaaa accgtggtag caatgtacac
    31561 aaccgggtgc ctgccagtgg ggcagcggcg acggccatac attgtctgga atatggatgg
    31621 gctccccttg ctgccggcga caatgcgggc aaagtttgcg taccagacgc tgggctatta
    31681 ccagcgtaag cgccgatgat agcatccagc gggcgactcc catttgctgt aaacgtacca
    31741 gcgtttcgca ggtggaatta gtgtgtaggg tagacaacac caggtgaccg gtttgcgcgg
    31801 cttttatggc aatttctgcc gtttcgccat cgcggatctc tccgatcatg atgacgtcag
    31861 gatcctggcg caataacgca cgcaaaacgc cctgaaaggt gagtccggca cgcgaatgga
    31921 tttgcgtctg gtttaatccg gctatgggga tctcaaccgg atcttcgacg ctacaaatat
    31981 taatgtcagc ggtattcagc gtttgcaggg cactataaag cgtgaccgtt ttgccgctgc
    32041 ctgtagggcc agttaccagc accagtccct gtggttgttg caaggcatga gcaaagtcca
    32101 ccagttgtga cggctgcatt ccaagcgtgt taacatccag tgcctggttc acctgctgta
    32161 acaaccttaa taccaccttt tcaccacccc gacatgctaa ggtcgcaata cgaaatgaga
    32221 cggcgtttcc tgccagttcg acagtgaatt gcccgtcctg cggcaggcga tgttccgcaa
    32281 tatccaggtt tcccagcact tttaatctgg cggttaatgc gactccggca tccggtgaaa
    32341 cgtccggtaa aggatgcaat acgccgtcga tacgcaagcg gatgcggtag gcattgtccg
    32401 ctggttcgat atgaatatca gacgcgcgtt gttctagcgc agattgtaac gttcgagtca
    32461 gcaactctgc tttgggctga tgcttctcct gaacagctac gggcaatgtc tgttgtgtgc
    32521 gactggcgtg accttccatt tgttggcgcg tccagcaggt gatctcaata cgtttggtgg
    32581 tagcgaaatg caatgcgtcc agtagctcat gcgaaggtgc atcgactacc gcaacatgaa
    32641 ccacctcttc gctggcatcc agcaagactc cctgataacg cagacacagg gcagtgagct
    32701 gtggaatatt cattgccgct ccttagttgg cgtcatcaaa gcggaagaca tcttcgcagg
    32761 cttgctgcaa tgcgctgtca ctttgaatat tgcagttacg cgtccagccg gtgacgccgt
    32821 ttgcgttatc ccaacccggt gtcatgacga cgcttagccc attgagactt tcttgcccgg
    32881 tcagcgacac cacgcccttt gccacactca tggctgaaac atagcgggtg gtggtaggcg
    32941 aaggaatgcc attgctgcca ccgtcgcagg tatttaatcc accatgttcc agcgcgcaca
    33001 actctacggc ggtacggtaa ggcacaaagg tttgtagcat gtcggtgagt gcggctttgc
    33061 gcaggtagtt ttgataagcg ggaataccaa tggcgcttaa aatggcaatg atgccaataa
    33121 ccaccatcag ttcaataagt gtaaaaccgc gttgcttgtc catctgtttc gctccttgat
    33181 ttggttggcg ctactttggc aaacgccatc agcacaagcg aggggcaaaa aacgaaacgg
    33241 gaaagcagct tccgaggttt tttacttcgt tgcagcgaaa gacaagaaat ttgcgaggcg
    33301 ttacgaagaa agttggggaa ggggagattt atccgcccgc gatggagcgg ataaatctgt
    33361 tagcaattag cgaaaacgca ttgaaaggtc gagtgcctgt acgtgtttag ttagtgcacc
    33421 aacggagata aagtccacgc ccgtttcggc aaattcgcgc agtgttttgt cagtgacgtt
    33481 gccagacact tccagtagcg ccttgccgtt ggtgcgtttg acggcttcgc gcatctgttc
    33541 tgtttcgaag ttatccagca tgatgatatc ggcacctgct ttcagggctt catcaagttc
    33601 ttccagattc tctacttcga cttctactgg cgcatccggg tgcaaccagg acgctttttc
    33661 gaccgcctgc cgcactgagc cggaggcaat aatatggttt tctttgatca ggaaggcatc
    33721 agaaagcccc agacggtgat tcgctccgcc gccgcaaagt accgcgtatt tcagagctga
    33781 acgcaggccg ggtaaggttt tgcgcgtatc caacagctgc gtgttggtgc cttccagtaa
    33841 ttcgacatag tggcgtacct tactggcaac tcctgaaagg gtttgcacaa aattaagcgc
    33901 agtgcgttcg cccgttaaca gcacgcgaga tgggccttca agttcgaaca agggttgatt
    33961 ggcgttgatg acatcgccgt catccacatg ccagattatg gtgacatcgt cgcctgccag
    34021 ttgaataaac acctcttcaa cccagcgttt gccgcaaaat acgccattct cgcgggtgat
    34081 cactgtggca tgagagcgag agttttccgg taaaagtttt gccgtaatgt cattgttggc
    34141 atcgactgtt ccgcctaaat cttcccgcag cgcctgggcc accgcgccgg ggatatcgag
    34201 attaatgcgt tccagcagct cgtcacgtcg ggtgtcaggg ttatagcggc gaggcggcat
    34261 gttaaaactc cagatagcta acgaatcata aggtagaaac atgctactct gaaccgggta
    34321 ttagcaccac atataaggag atcctgcatg ttgttagaac aggggtggct ggctggcgcg
    34381 cgccgcgttc cctcaccaca ttacgattgc cgcccggatg acgaaacacc caccctgctg
    34441 gtggtgcaca atattagcct gccgccaggc gagtttggcg gtccgtggat cgacgcatta
    34501 ttcactggaa ctattgatcc gcaggcgcat cctttctttg cggagatcgc ccatttgcgc
    34561 gtctccgctc actgtttgat tcgccgtgat ggtgaaatag tccagtatgt tcctttcgat
    34621 aaacgagcct ggcatgcggg agtctctcag tatcaggggc gcgaacgctg caatgatttt
    34681 tctattggga ttgagctgga aggcaccgat acgctggcgt ataccgatgc gcagtatcaa
    34741 cagcttgcgg cggttacacg ggcactgatt gatcgctatc cggatatcgc taataacatg
    34801 acgggccatt gtgatattgc gccggatcgg aaaaccgatc ccggtcctgc atttgattgg
    34861 gcacggtttc gtgcgctggt cagcaaggag acaacatgac gctatttaca accttactgg
    34921 tgttaatttt cgagcgcctg tttaagttgg gcgagcactg gcagcttgat catcgtcttg
    34981 aagcgttctt tcggcgggtg aaacattttt ctctcgggcg cacgttatgc atgaccatta
    35041 ttgcgatggg cgtgactttt ttactgttac gcgcattgca gggtgtattg ttcaacgttc
    35101 ccacgttgct ggtgtggctg ctgattggtt tgctgtgtat tggcgcaggt aaagttcgtc
    35161 ttcattatca tgcttatctg acagctgctt cacgtaatga tagccatgcc catgccacga
    35221 tggctggcga actcaccatg attcacggcg tcccggcagg ctgcgatgaa cgtgagtatt
    35281 tgcgtgagct gcaaaatgca ttgctgtgga ttaactttcg tttttatctt gctccgctgt
    35341 tctggctgat tgtgggggga acctggggac ccgttacgct gatggggtat gcgtttttgc
    35401 gtgcatggca atactggctg gcgcgatacc agacgccgca tcatcgttta cagtccggca
    35461 ttgatgccgt gcttcatgca ctggattggg tgccggttcg tcttgcgggt gtggtatatg
    35521 ccttgatcgg tcatggtgag aaagcgttac cggcctggtt tgcttcgctg ggtgatttcc
    35581 atacttcgca atatcaggta ttaacgcgtc tggcacagtt ctctctggcg cgtgaaccgc
    35641 atgtcgataa ggtggagacg ccaaaggccg cggtttcaat ggcgaagaaa acctcgttcg
    35701 tggtcgtggt ggtaattgcg ctactgacga tttacggggc gttggtgtaa agattattgc
    35761 cctcaccctg cacgggtgag ggcgtagaga gattaatgcg cttttacggc tttggcggtt
    35821 ttctctttaa acagatagcc gatacctaac acgatcagcc ataccgggat caggtatacc
    35881 gaaatcgcca ttcctggggt catcagcata atcaccagta ccgccgccat aaacagcagg
    35941 cagatccagt tacccagcgg ataaagcagg gcagggaagc gagttaccac gccttgttct
    36001 tgtttggcac gacggaattt catatgcgcc agactgatca tcgcccagtt gatgaccagt
    36061 gcagatacca ccagcgccat taacagtccg aaagcggact ctggggcaag gtagttaatc
    36121 agtacgcaca atgccgtaac cagcgcagac accagaatag tgttaaccgg tacgccgcgt
    36181 ttatcgacag acgccagcgc ttttggcgcg ttcccttgtt gtgccagacc gaacagcata
    36241 cggctgttgc aatatacgca gctgttgtac acggagagcg cagcagtcag taccacgata
    36301 ttcagcgcat tcgctacaaa ggtatcgcct aactcgtgga agatcagcac aaacggactg
    36361 gtatcggcgg taacgcgggt ccacggcatt agtgagagca gaacggctaa cgagccaata
    36421 tagaaaatca ggatgcggta gataacctgg ttagttgctt tcgggatact ttgctccggg
    36481 ttatcagctt ctgctgcggt gatccccacc agttccagac caccgaaaga gaacatgata
    36541 atcgccatca tcatcaccag accggtgaag ccgtgtggca ggaaaccacc ctgatcccac
    36601 aggttgctaa cgctcgcctg cgggccgccg ttgccgctga atagcagcca ggcgccgaag
    36661 atgatcatcg ctaccaccgc gataactttg ataatggcaa accagaactc catctcgcca
    36721 aacactttta cgtttgtcag gttgatggcg ttgatcacca caaagaacac ggcggcagaa
    36781 acccaggttg ggatttccgg ataccagaac tgaatgtatt tacccacggc agtcagctct
    36841 gccatggcaa ctaaaacgta cagtacccag tagttccagc cagaggcgaa accggcaaaa
    36901 ctgccccagt atttataagc aaagtggcta aaggagcctg cgacaggttc ttcgaccacc
    36961 atttcaccta gctgacgcat gatcagaaag gcgataaaac cagcaatggc gtaacccagg
    37021 ataatccctg gccctgcgga ctgtattacg gaggcgctac ccaggaatag ccctgtccct
    37081 atcgcgccac ccagcgcgat aagctgaata tggcggtttt taaggccgcg ctttagctgc
    37141 tcgccgtgct gttgaccttc cattatgaaa cctcgtgttg tgttgttttt ttgaactacg
    37201 cagcaatgcg tgtgtaattt tgtaattccg tttgttgtat tggtttcttt acattttatg
    37261 taactgaatt atgatgatag tactttcaac tatcacaaga taatggtata cgcgcgagaa
    37321 tgcacctgct ttatgtagat gtgttgattt tctggataaa aaaaaagcaa ttgtaagttt
    37381 ttcatcacat ttaatagata ctttaagtct gataacatac tgttttaact agctatatac
    37441 cggatttgtc tgcctgctga acaccatgcc ttagccacca g
//
LOCUS       DAHHTG010000035        36719 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.035, whole genome shotgun sequence.
ACCESSION   DAHHTG010000035 DAHHTG010000000
VERSION     DAHHTG010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 36719)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36719)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36719
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.035"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            304..1029
                     /locus_tag="NBI74_001737"
     CDS             304..1029
                     /locus_tag="NBI74_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCD1932095.1"
                     /translation="MLPGCCKNGIVISKIPVMQAGLKEVMRTHFPEYEIISSASAEDL
                     TLLQLRRSGLVIADLAGESEDPRSVCEHYYSLISQYREIHWVFMVSRSWYSQAVELLM
                     CPTATLLSDVEPIENLVKTVRSGNTHAERISAMLTSPAMTETHDFSYRSVILTLSERK
                     VLRLLGKGWGINQIASLLKKSNKTISAQKNSAMRRLAIHSNAEMYAWINSAQGARELN
                     LPSVYGDAAEWNTAELRREMSHS"
     gene            987..1664
                     /gene="bglJ"
                     /locus_tag="NBI74_001738"
     CDS             987..1664
                     /gene="bglJ"
                     /locus_tag="NBI74_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418786.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator BglJ"
                     /protein_id="HCD1932096.1"
                     /translation="MEHSRIKKRNVALIEKCVMSSIGIESLFRKFAGNPYKLHTYTSQ
                     ESFQDAMTRISFAAVIFSFSAMRSERREGLSCLTELAIKFPRTRRLVIADDDIEARLI
                     GSLSPSPLDGVLSKASTLEIFHQELFLSLNGVRQATDRLNNQWYINQSRTLSPTEREI
                     LRFMSRGYSMTQIAEQLKRNIKTIRAHKFNVMSKLGVSSDAGLLEAADILLCMRHCEA
                     SNVLHPY"
     gene            complement(1702..2490)
                     /gene="fhuF"
                     /locus_tag="NBI74_001739"
     CDS             complement(1702..2490)
                     /gene="fhuF"
                     /locus_tag="NBI74_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore-iron reductase FhuF"
                     /protein_id="HCD1932097.1"
                     /translation="MAYRSAPLYEDIIWRTHLQPQDAGLAQAVRATIAKHREHLLEFI
                     RLDEPAPLNAMTLAQWSSPNALSSLLAVYSDHIYRNQPTMIRENKPLISLWAQWYIGL
                     MVPPLMLALLTQEKALDVSPEHFHAEFHETGRVACFWVDVCEDKNATPHSPQQRMETL
                     ISQALVPVVQALEATGEINGKLIWSNTGYLINWYLTEMKQLLGEATVESLRHALFFEK
                     TLTNGEDNPLWRTVVLRDGLLVRRTCCQRYRLPDVQQCGDCTLK"
     gene            2631..2867
                     /locus_tag="NBI74_001740"
     CDS             2631..2867
                     /locus_tag="NBI74_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313355.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1435 domain-containing protein"
                     /protein_id="HCD1932098.1"
                     /translation="MLQRTLGSGWGVLLPGLLIAGLMYADLSPDQWRIVILMGLILTP
                     VMLYHKQLRHYILLPSCLALSAGIMLMLMNLNQG"
     gene            complement(2906..2992)
                     /locus_tag="NBI74_001741"
     tRNA            complement(2906..2992)
                     /locus_tag="NBI74_001741"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2956..2958),aa:Leu,seq:cag)
     gene            complement(3301..4332)
                     /gene="rsmC"
                     /locus_tag="NBI74_001742"
     CDS             complement(3301..4332)
                     /gene="rsmC"
                     /locus_tag="NBI74_001742"
                     /EC_number="2.1.1.172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1207)-N(2))-methyltransferase
                     RsmC"
                     /protein_id="HCD1932099.1"
                     /translation="MSAFTPASEVLLRHSDDFEQSRILFAGDLQDDLPARLDTAASRA
                     HTQQFHHWQVLSRQMGDNARFSLVATVDDVADCDTLIYYWPKNKPEAQFQLMNLLSLL
                     PVGTDIFVVGENRSGVRSAEQMLADYAPLNKVDSARRCGLYFGRLEKQPVFDADKFWG
                     EYSVDGLTVKTLPGVFSRDGLDVGSQLLLSTLTPHTKGKVLDVGCGAGVLSVAFARHS
                     PKIRLTLCDVSAPAVEASRATLAANGVEGEVFASNVFSEVKGRFDMIISNPPFHDGMQ
                     TSLDAAQTLIRGAVRHLNSGGELRIVANAFLPYPDVLDETFGFHEVIAQTGRFKVYRA
                     IMTRQAKKG"
     gene            4435..4848
                     /gene="holD"
                     /locus_tag="NBI74_001743"
     CDS             4435..4848
                     /gene="holD"
                     /locus_tag="NBI74_001743"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit psi"
                     /protein_id="HCD1932100.1"
                     /translation="MTSRRDWQLQQLGITQWSLRRPGALQGEIAIAIPAHVRLVMVAN
                     DLPALTDPLVSDVLRALTVSPDQVLQLTPEKIAMLPQGSRCNSWRLGTDEPLSLEGAQ
                     VASPALTELRANPTARAALWQQICTYEHDFFPRND"
     gene            4817..5263
                     /gene="rimI"
                     /locus_tag="NBI74_001744"
     CDS             4817..5263
                     /gene="rimI"
                     /locus_tag="NBI74_001744"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HCD1932101.1"
                     /translation="MNTISSLETTDLPAAYHIEQRAHAFPWSEKTFASNQGERYLNFQ
                     LTQNGKMAAFAITQVVLDEATLFNIAVDPDYQRQGLGRVLLEHLIDELEKRGVATLWL
                     EVRASNAAAIALYESLGFNEATIRRNYYPTTDGREDAIIMALPISM"
     gene            5278..5955
                     /gene="yjjG"
                     /locus_tag="NBI74_001745"
     CDS             5278..5955
                     /gene="yjjG"
                     /locus_tag="NBI74_001745"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HCD1932102.1"
                     /translation="MKWDWIFFDADETLFTFDSFTGLQRMFLDYSVTFTAEDFQDYQA
                     VNKPLWVDYQNGAITSLQLQHGRFESWAERLNVEPGKLNEAFINAMAEICTPLPGAVS
                     LLNAIRGNAKIGIITNGFSALQQVRLERTGLRDYFDLLVISEEVGVAKPNKKIFDYAL
                     EQAGNPDRSRVLMVGDTAESDILGGINAGLATCWLNAHNREQPEGIAPTWTVSSLHEL
                     EQLLCKH"
     gene            6046..7635
                     /gene="prfC"
                     /locus_tag="NBI74_001746"
     CDS             6046..7635
                     /gene="prfC"
                     /locus_tag="NBI74_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HCD1932103.1"
                     /translation="MTLSPYLQEVAKRRTFAIISHPDAGKTTITEKVLLFGQAIQTAG
                     TVKGRGSNQHAKSDWMEMEKQRGISITTSVMQFPYHDCLVNLLDTPGHEDFSEDTYRT
                     LTAVDCCLMVIDAAKGVEDRTRKLMEVTRLRDTPILTFMNKLDRDIRDPMELLDEVEN
                     ELKIGCAPITWPIGCGKLFKGVYHLYKDETYLYQSGKGHTIQEVRIVKGLNNPDLDAA
                     VGEDLAQQLRDELELVKGASNEFDKELFLAGEITPVFFGTALGNFGVDHMLDGLVEWA
                     PAPMPRQTDTRTVEASEDKFTGFVFKIQANMDPKHRDRVAFMRVVSGKYEKGMKLRQV
                     RTAKDVVISDALTFMAGDRSHVEEAYPGDILGLHNHGTIQIGDTFTQGEMMKFTGIPN
                     FAPELFRRIRLKDPLKQKQLLKGLVQLSEEGAVQVFRPISNNDLIVGAVGVLQFDVVV
                     SRLKSEYNVEAVYESVNVATARWVECADAKKFEEFKRKNESQLALDGGDNLAYIATSM
                     VNLRLAQERYPDVQFHQTREH"
     gene            8028..8633
                     /gene="osmY"
                     /locus_tag="NBI74_001747"
     CDS             8028..8633
                     /gene="osmY"
                     /locus_tag="NBI74_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone OsmY"
                     /protein_id="HCD1932104.1"
                     /translation="MTMTRLKISKTLLAVMLTSAVATGSAYAENNAQTTNESAGQKVD
                     SSMNKVGNFMDDSAITAKVKAALVDHDNIKSTDISVKTDQKVVTLSGFVESQAQAEEA
                     VKVAKGVEGVTSVSDKLHVRDAKEGSVKGYAGDTATTSEIKAKLLADDIVPSRHVKVE
                     TTDGVVQLSGTVDSQAQSDRAESIAKAVDGVKSVKNDLKTK"
     gene            8760..8921
                     /locus_tag="NBI74_001748"
     CDS             8760..8921
                     /locus_tag="NBI74_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002887716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 domain-containing protein"
                     /protein_id="HCD1932105.1"
                     /translation="MFRWGIIFLVIALIAAALGFGGLAGTAAGAAKIVFVVGIILFLV
                     SLFMGRKRP"
     gene            9043..10116
                     /locus_tag="NBI74_001749"
     CDS             9043..10116
                     /locus_tag="NBI74_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin family protein"
                     /protein_id="HCD1932106.1"
                     /translation="MGQRIPVTLGNIAPLSLRPFQPGRIALVCEGGGQRGIFTAGVLD
                     EFMRAQFNPFDLYLGTSAGAQNLSAFICNQPGYARKVIMRYTTKREFFDPLRFVRGGN
                     LIDLDWLVEATASQMPLQMDTAARLFDSGKSFYMCACRQDDYAPNYFLPTKQNWLDVI
                     RASSAIPGFYRSGVSLEGINYLDGGISDAIPVKEAARQGAKTLVVIRTVPSQMYYTPQ
                     WFKRMERWLGDSSLQPLVNLVQHHETSYRDIQQFIEKPPGKLRIFEIYPPKPLHSIAL
                     GSRIPALREDYKLGRLCGRYFLATVGKLLTEKAPLTRHLVPVVTPESIVIPPAPVAND
                     TLVAEVSDAPQANDPTFNNEDLA"
     gene            10113..10895
                     /locus_tag="NBI74_001750"
     CDS             10113..10895
                     /locus_tag="NBI74_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HCD1932107.1"
                     /translation="MICRFIDTHCHFDFPPFSGDEEASLQRAAQAGVGKIIVPATEAE
                     NFARVQALAEKYQPLYAALGLHPGMLEKHSDVSLDQLQQALERRPAKVVAVGEIGLDL
                     FGDDPQFERQQWLLDEQLKLAKRYDLPVILHSRRTHDKLAMHLKRHDLPCTGVVHGFS
                     GSLQQAERFVQLGYKIGVGGTITYPRASKTRDVIAKLPLASLLLETDAPDMPLNGFQG
                     QPNRPEQAVRVFDVLCELRPEPEDEIAEVLLNNTYALFSVSG"
     gene            complement(11412..12275)
                     /locus_tag="NBI74_001751"
     CDS             complement(11412..12275)
                     /locus_tag="NBI74_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjW family glycine radical enzyme activase"
                     /protein_id="HCD1932108.1"
                     /translation="MNSRCALVSKIIPFSCVDGPGSRLALFLQGCNLRCKNCHNPWTM
                     GRCNDCGECVPQCPHQALQIVDGKVLWNAVVCEQCDTCLKMCPQHATPMAQSMSVDEV
                     LSHIRKAVLFIEGITVSGGEATTQLPFVVALFTAIKNDPQLRHLTCLVDSNGMLSETG
                     WEKLLPVCDGAMLDLKAWGSECHQQLTGRDNQQIKRSICLLAERGKLAELRLLVIPGQ
                     VDYLQHIEELAVFIKGLGDVPVRLNAFHAHGVYGEAQSWASATPEDVEPLADALKVRG
                     VSRLIFPALYL"
     gene            complement(12247..13797)
                     /locus_tag="NBI74_001752"
     CDS             complement(12247..13797)
                     /locus_tag="NBI74_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjI family glycine radical enzyme"
                     /protein_id="HCD1932109.1"
                     /translation="MPTSHENALQQRCQQIVTSPVLSPEQKRHFLALEAENNLPYPQL
                     PAEARRALDEGVICDMFEGHAPYKPRYVLPDYARFLANGSEWLELEGAKDLDDALSLL
                     TILYHHVPSVTSMPVYLGQLDALLQPYVRILTQNEIDIRIKRFWRYLDRTLPDAFMHA
                     NIGPSDSPITRAILRADAELKQVSPNLTFIYDPEITPDDLLLEVAKNICECSKPHIAN
                     GPVHDKIFTNGGYGIVSCYNSLPLAGGGSTLVRLNLKAIAERSESLDDFFTRTLPHYC
                     QQQIAIIDARCEFLYQQSHFFENSFLVKEGLINPERFVPMFGMYGLAEAVNLLCEKEG
                     IAARYGKEAAANEVGYRISAQLAEFVANTPVKYGWQNRAMLHAQSGISSDIGTTPGAR
                     LPYGDEPDPITHLQTVAPHHAYYYSGISDILTLDETIKRNPQALVQLCLGAFKAGMRE
                     FTANVSGNDLVRVTGYMVRLSDLEKYRAEGSRTNTTWLGEEAARNTRILERQPRVISH
                     EQQMRFSQ"
     gene            14056..14835
                     /gene="deoC"
                     /locus_tag="NBI74_001753"
     CDS             14056..14835
                     /gene="deoC"
                     /locus_tag="NBI74_001753"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005097947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HCD1932110.1"
                     /translation="MTDLKASSLRALKLMDLTTLNDDDTDEKVIALCHQAKTPVGNTA
                     AICIYPRFIPIARKTLKEQGTPEIRIATVTNFPHGNDDIEIALAETRAAIAYGADEVD
                     VVFPYRALMAGNEQVGFDLVKACKEACAAANVLLKVIIETGELKDEALIRKASEISIK
                     AGADFIKTSTGKVAVNATPESARIMMEVIRDMGVEKTVGFKPAGGVRTAEDAQKYLAI
                     ADELFGADWADARHYRFGASSLLASLLKALGHGDGKSASSY"
     gene            14999..16321
                     /gene="deoA"
                     /locus_tag="NBI74_001754"
     CDS             14999..16321
                     /gene="deoA"
                     /locus_tag="NBI74_001754"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313368.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HCD1932111.1"
                     /translation="MFLAQEIIRKKRDGHALSDEEIRFFINGIRDNTISEGQIAALAM
                     TIFFHDMTMPERVSLTMAMRDSGTVLDWKSLHLNGPIVDKHSTGGVGDVTSLMLGPMV
                     AACGGYIPMISGRGLGHTGGTLDKLESIPGFDIFPDDNRFREIIKDVGVAIIGQTSSL
                     APADKRFYATRDITATVDSIPLITASILAKKLAEGLDALVMDVKVGSGAFMPTYELSE
                     ALAEAIVGVANGAGVRTTALLTDMNQVLASSAGNAVEVREAVQFLTGEYRNPRLFDVT
                     MALCVEMLTSGKLAKDDAEARAKLQAVLDNGKAAEVFGRMVAAQKGPTDFVENYAKYL
                     PTAMLTKAVYADTEGFVSEMDTRALGMAVVAMGGGRRQASDTIDYSVGFTYMARLGDQ
                     VDGQRPLAVIHAKDENSWQEAAKAVKAAIKLADKAPESTPTVYRRISE"
     gene            16373..17596
                     /gene="deoB"
                     /locus_tag="NBI74_001755"
     CDS             16373..17596
                     /gene="deoB"
                     /locus_tag="NBI74_001755"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HCD1932112.1"
                     /translation="MKRAFIMVLDSFGIGATEDAERFGDVGADTLGHIAEACAKGEAD
                     NGRKGPLNLPNLTRLGLAKAHEGSTGFIPAGMDGNAEVIGAYAWAHEMSSGKDTPSGH
                     WEIAGVPVLFEWGYFSDHENSFPQELLDKLVERANLPGYLGNCHSSGTVILDQLGEEH
                     MKTGKPIFYTSADSVFQIACHEETFGLDKLYELCEIAREELTNGGYNIGRVIARPFIG
                     DKAGNFQRTGNRHDLAVEPPAPTVLQKLVDEKHGQVVSVGKIADIYANCGITKKVKAT
                     GLDALFDATIKEMKEAGDNTIVFTNFVDFDSSWGHRRDVAGYAAGLELFDRRLPELMS
                     LLRDDDILILTADHGCDPTWTGTDHTREHIPVLVYGPKVKPGSLGHRETFADIGQTLA
                     KYFGTSDMEYGKAMF"
     gene            17653..18372
                     /gene="deoD"
                     /locus_tag="NBI74_001756"
     CDS             17653..18372
                     /gene="deoD"
                     /locus_tag="NBI74_001756"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HCD1932113.1"
                     /translation="MATPHINAEMGDFADVVLMPGDPLRAKYIAETFLEDAREVNNVR
                     GMLGFTGTYKGRKISVMGHGMGIPSCSIYTKELITDFGVKKIIRVGSCGAVLPHVKLR
                     DVVIGMGACTDSKVNRIRFKDHDFAAIADFDMVRNAVDAAKALGIDARVGNLFSADLF
                     YSPDGEMFDVMEKYGILGVEMEAAGIYGVAAEFGAKALTICTVSDHIRTHEQTTAAER
                     QTTFNDMIKIALESVLLGDKE"
     gene            complement(18533..18796)
                     /locus_tag="NBI74_001757"
     CDS             complement(18533..18796)
                     /locus_tag="NBI74_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCD1932114.1"
                     /translation="MIDLKNQEREIINLMFSQGISWLTAVRIRHKLSLAEVSKMLGIS
                     INSLKQIEKTERLSSNIKNKMAGIYGCPPELLICPYWMTAEHK"
     gene            complement(18828..19844)
                     /gene="lplA"
                     /locus_tag="NBI74_001758"
     CDS             complement(18828..19844)
                     /gene="lplA"
                     /locus_tag="NBI74_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase LplA"
                     /protein_id="HCD1932115.1"
                     /translation="MSTLRLLISDSYDPWFNLAVEECIFRQMPATQRVLFLWRNADTV
                     VIGRAQNPWKECNTRRMEEDNVRLARRSSGGGAVFHDLGNTCFTFMAGKPEYDKTIST
                     SIVLNALNALGVSAEASGRNDLVVKTAEGDRKVSGSAYRETKDRGFHHGTLLLNADLS
                     RLANYLNPDKKKLAAKGITSVRSRVTNLTELLPGITHEQVCEAISEAFFAHYGERVEA
                     EIISPDKTPDLPNFAETFARQSSWEWNFGQAPAFSHLLDERFTWGGVELHFDVEKGHI
                     TRAQVFTDSLNPAPLEALAGRLQGCLYRADMLQQECEALLVDFPEQEKELRELSAWMA
                     GAVR"
     gene            complement(19872..20516)
                     /locus_tag="NBI74_001759"
     CDS             complement(19872..20516)
                     /locus_tag="NBI74_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtjB family periplasmic protein"
                     /protein_id="HCD1932116.1"
                     /translation="MARTKLKFRLHRAVIVLFCLALLVALMQGASWFSQNHQRQRNPQ
                     LEELARTLARQVTLNVAPLMRTDSPDEKRIQAILDQLTDESRILDAGVYDEQGDLIAR
                     SGESVEVRDRLALDGKKAGGYFNQQIVEPIAGKNGPLGYLRLTLDTHTLATEAQQVDN
                     TTNILRLMLLLSLAIGVVLTRTLLQGKRTRWQQSPFLLTASKPVPEEEESEKKE"
     gene            20622..21590
                     /gene="serB"
                     /locus_tag="NBI74_001760"
     CDS             20622..21590
                     /gene="serB"
                     /locus_tag="NBI74_001760"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase"
                     /protein_id="HCD1932117.1"
                     /translation="MPNITWCDLPEDVSLWPGLPLSLSGDEVMPLDYHAGRSGWLLYG
                     RGLDKQRLTQYQSKLGAAMVIVAAWCVEDYQVIRLAGSLTARATRLAHEAQLDVAPLG
                     KIPHLRTPGLLVMDMDSTAIQIECIDEIAKLAGTGEMVAEVTERAMRGELDFTASLRS
                     RVATLKGADANILQQVRENLPLMPGLTQLVLKLETLGWKVAIASGGFTFFAEYLRDKL
                     RLTAVVANELEIMDGKFTGNVIGDIVDAQYKAKTLTRLAQEYEIPLAQTVAIGDGAND
                     LPMIKAAGLGIAYHAKPKVNEKTEVTIRHADLMGVFCILSGSLNQK"
     gene            21639..23021
                     /gene="radA"
                     /locus_tag="NBI74_001761"
     CDS             21639..23021
                     /gene="radA"
                     /locus_tag="NBI74_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001399683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HCD1932118.1"
                     /translation="MAKAPKRAFVCNECGADYPRWQGQCSACHAWNTITEVRLAASPT
                     VARNERLSGYAGSAGVAKVQKLSDISLEELPRFSTGFKEFDRVLGGGVVPGSAILIGG
                     NPGAGKSTLLLQTLCKLAQQMKTLYVTGEESLQQVAMRAHRLGLPTDNLNMLSETSIE
                     QICLIAEEEQPKLMVIDSIQVMHMADVQSSPGSVAQVRETAAYLTRFAKTRGVAIVMV
                     GHVTKDGSLAGPKVLEHCIDCSVLLDGDADSRFRTLRSHKNRFGAVNELGVFAMTEQG
                     LREVSNPSAIFLSRGDEVTSGSSVMVVWEGTRPLLVEIQALVDHSMMANPRRVAVGLE
                     QNRLAILLAVLHRHGGLQMADQDVFVNVVGGVKVTETSADLALLLAMVSSLRDRPLPQ
                     DLVVFGEVGLAGEIRPVPSGQERISEAAKHGFRRAIVPAANVPKKAPEGMQIFGVKKL
                     SDALSVFDDL"
     gene            23042..24274
                     /gene="nadR"
                     /locus_tag="NBI74_001762"
     CDS             23042..24274
                     /gene="nadR"
                     /locus_tag="NBI74_001762"
                     /EC_number="2.7.1.22"
                     /EC_number="2.7.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463436.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional transcriptional
                     regulator/nicotinamide-nucleotide
                     adenylyltransferase/ribosylnicotinamide kinase NadR"
                     /protein_id="HCD1932119.1"
                     /translation="MSSFDYLKTAIKQQGCTLQQVADASGMTKGYLSQLLNAKIKSPS
                     AQKLEALHRYLGLEFPRQKKTIGVVFGKFYPLHTGHIYLIQRACSQVDELHIIMGFDD
                     TRDRALFEDSAMSQQPTVPDRLRWLLQTFKYQKNIRIHAFNEEGMEPYPHGWDVWSNG
                     IKKFMAEKGIQPDLIYTSEEADAPQYMEHLGIETVLVDPKRTFMSISGAQIRENPFRY
                     WEYIPTEVKPFFVRTVAILGGESSGKSTLVNKLANIFNTTSAWEYGRDYVFSHLGGDE
                     IALQYSDYDKIALGHAQYIDFAVKYANKVAFIDTDFVTTQAFCKKYEGREHPFVQALI
                     DEYRFDLVILLENNTPWVADGLRSLGSSVDRKEFQNLLVEMLEENNIEFVRVEEDDYD
                     SRFLRCVELVREMMGEQG"
     gene            24366..24698
                     /locus_tag="NBI74_001763"
     CDS             24366..24698
                     /locus_tag="NBI74_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015873600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HCD1932120.1"
                     /translation="MGKAIIFIETPMFTRQIKQIATDDELKELQKELIGTPDKGDLIQ
                     QTGGLRKVRMAAGSQGKSGSLRIIYFLATEEIIYLIMAYPKNAKDSLTDTEKAQLKKL
                     TKLLKGEV"
     gene            24700..24984
                     /locus_tag="NBI74_001764"
     CDS             24700..24984
                     /locus_tag="NBI74_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCD1932121.1"
                     /translation="MSFFDELKTSLEEAVEIKQGLKEPARVTRYEIADVKAIREQLNV
                     SQSEMAKALGTSVDTIKSWESKRRNPTGLAAKVLATIKENPAFFRELASH"
     gene            complement(25040..26707)
                     /gene="ettA"
                     /locus_tag="NBI74_001765"
     CDS             complement(25040..26707)
                     /gene="ettA"
                     /locus_tag="NBI74_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017145039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HCD1932122.1"
                     /translation="MAQFVYTMHRVGKVVPPKRHILKNISLSFFPGAKIGVLGLNGAG
                     KSTLLRIMAGIDKDIEGEARPQPDIKIGYLPQEPQLNPEHTVRESIEEAVSEVVNALK
                     RLDEVYALYADPDADFDKLAAEQGRLEEIIQAHDGHNLNVQLERAADALRLPDWDAKI
                     ANLSGGERRRVALCRLLLEKPDMLLLDEPTNHLDAESVAWLERFLHDFEGTVVAITHD
                     RYFLDNVAGWILELDRGEGIPWEGNYSSWLEQKDQRLAQEASQEAARRKSIEKELEWV
                     RQGTKGRQSKGKARLARFEELNSTEYQKRNETNELFIPPGPRLGDKVLEVSNLRKSYG
                     DRLLIDDLSFSIPKGAIVGIIGPNGAGKSTLFRMISGQEQPDSGTITLGETVKLASVD
                     QFRDSMDNSKTVWEEVSGGLDIMKIGNTEMPSRAYVGRFNFKGVDQGKRVGELSGGER
                     GRLHLAKLLQVGGNMLLLDEPTNDLDIETLRALENALLEFPGCAMVISHDRWFLDRIA
                     THILDYQDEGKVEFFEGNFTEYEEYKKRTLGADALEPKRIKYKRIAK"
     gene            26918..28855
                     /gene="sltY"
                     /locus_tag="NBI74_001766"
     CDS             26918..28855
                     /gene="sltY"
                     /locus_tag="NBI74_001766"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase"
                     /protein_id="HCD1932123.1"
                     /translation="MEKAKHVTWRLLAAGVCLLTVSSVARADSLDEQRSRYAQIKQAW
                     DNRQMDVVEQMMPGLKDYPLYPYLEYRQITDDLMNQPAVTVTNFVRANPTLPPARTLQ
                     SRFVNELARREDWRGLLAFSPEKPGTTEAQCNYYYAKWNTGQSEEAWQGAKELWLTGK
                     SQPNACDKLFSVWRASGKQDPLAYLERIRLAMKAGNTGLVTVLAGQMPADYQTIASAI
                     ISLANNPNTVLTFARTTGATDFTRQMAAVAFASVARQDAENARLMIPSLAQAQQLNED
                     QIQELRDIVAWRLMGNDVTDEQAKWRDDAIMRSQSTSLIERRVRMALGTGDRRGLNTW
                     LARLPMEAKEKDEWRYWQADLLLERGREAEAKEILHQLMQQRGFYPMVAAQRIGEEYE
                     LKIDKAPQNVDSALTQGPEMARVRELMYWNLDNTARSEWANLVKSKSKTEQAQLARYA
                     FNNQWWDLSVQATIAGKLWDHLEERFPLAYNDLFKRYTSGKEIPQSYAMAIARQESAW
                     NPKVKSPVGASGLMQIMPGTATHTVKMFSIPGYSSPGQLLDPETNINIGTSYLQYVYQ
                     QFGNNRIFSSAAYNAGPGRVRTWLGNSAGRIDAVAFVESIPFSETRGYVKNVLAYDAY
                     YRYFMGDKPTLMSATEWGRRY"
     gene            28945..29271
                     /gene="trpR"
                     /locus_tag="NBI74_001767"
     CDS             28945..29271
                     /gene="trpR"
                     /locus_tag="NBI74_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon repressor"
                     /protein_id="HCD1932124.1"
                     /translation="MAQQSPYSAAMAEQRHQEWLRFVDLLKNAYQNDLHLPLLNLMLT
                     PDEREALGTRVRIVEELLRGEMSQRELKNELGAGIATITRGSNSLKAAPVELRQWLEE
                     VLLKDH"
     gene            complement(29265..29780)
                     /gene="yjjX"
                     /locus_tag="NBI74_001768"
     CDS             complement(29265..29780)
                     /gene="yjjX"
                     /locus_tag="NBI74_001768"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001649724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/xanthosine triphosphatase"
                     /protein_id="HCD1932125.1"
                     /translation="MHQVVCATTNPAKIQAILQAFHEIFGEGSCHIASVAVESGVPEQ
                     PFGSEETRAGARNRVANARRLHPEADFWVAIEAGIDGDSTFSWVVIENASQRGEARSA
                     TLPLPAVILEKVREGEALGPVMSRYTGIDEIGRKEGAIGVFTAGKLTRASVYHQAVIL
                     ALSPFHNAVYQ"
     gene            29832..30479
                     /gene="gpmB"
                     /locus_tag="NBI74_001769"
     CDS             29832..30479
                     /gene="gpmB"
                     /locus_tag="NBI74_001769"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase GpmB"
                     /protein_id="HCD1932126.1"
                     /translation="MLQVYLVRHGETQWNAERRIQGQSDSPLTAKGEQQAMQVATRAK
                     ELGITHIISSDLGRTRRTAEIIAQACGCDIIFDSRLRELNMGVLEKRHIDSLTEEEEN
                     WRRQLVNGTVDGRIPEGESMQELSDRVNAALESCRDLPQGSRPLLVSHGIALGCLVST
                     ILGLPAWAERRLRLRNCSISRVDYQESLWLASGWVVETAGDISHLDAPALDELQR"
     gene            complement(30476..31345)
                     /gene="robA"
                     /locus_tag="NBI74_001770"
     CDS             complement(30476..31345)
                     /gene="robA"
                     /locus_tag="NBI74_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     RobA"
                     /protein_id="HCD1932127.1"
                     /translation="MDQAGIIRDLLIWLEGHLDQPLSLDNVAAKAGYSKWHLQRMFKD
                     VTGHAIGAYIRARRLSKSAVALRLTARPILDIALQYRFDSQQTFTRAFKKQFAQTPAL
                     YRRSPEWSAFGIRPPLRLGEFTMPEHKFVTLEDTPLIGVTQSYSCSLEQISDFRHEMR
                     YQFWHDFLGNAPTIPPVLYGLNETRPSQDKDDEQEVFYTTALAQDQADGYVLTGHPVM
                     LQGGEYVMFTYEGLGTGVQEFILTVYGTCMPMLNLTRRKGQDIERYYPAEDAKAGDRP
                     INLRCELLIPIRR"
     gene            31556..32029
                     /gene="creA"
                     /locus_tag="NBI74_001771"
     CDS             31556..32029
                     /gene="creA"
                     /locus_tag="NBI74_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein CreA"
                     /protein_id="HCD1932128.1"
                     /translation="MKYKHLILSLSLIMLGPLAHAEEIGSVDTVFKMIGPDHKIVVEA
                     FDDPDVKNVTCYVSRAKTGGIKGGLGLAEDTSDAAISCQQVGPIELSDRIKNGKAQGE
                     VVFKKRTSLVFKSLQVVRFYDAKRNALAYLAYSDKVVEGSPKNAISAVPVMPWRQ"
     gene            32042..32731
                     /gene="creB"
                     /locus_tag="NBI74_001772"
     CDS             32042..32731
                     /gene="creB"
                     /locus_tag="NBI74_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CreB"
                     /protein_id="HCD1932129.1"
                     /translation="MQRETVWLVEDEQGIADTLVYMLQQEGFAVEVFERGLPVLDKAR
                     QQVPDVMILDVGLPDISGFELCRQLLALHPALPVLFLTARSEEVDRLLGLEIGADDYV
                     AKPFSPREVCARVRTLLRRVKKFSTPSPVIHIGHFELNEPAAQISWFDTPLTLTRYEF
                     LLLKTLLKSPGRVWSRQQLMDSVWEDAQDTYDRTVDTHIKTLRAKLRAINPDLSPINT
                     HRGMGYSLRGL"
     gene            32731..34155
                     /gene="creC"
                     /locus_tag="NBI74_001773"
     CDS             32731..34155
                     /gene="creC"
                     /locus_tag="NBI74_001773"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     CreC"
                     /protein_id="HCD1932130.1"
                     /translation="MRIGMRLLLGYFLLVAVAAWFVLAIFVKEVKPGVRRATEGTLID
                     TATLLAELARPDLLSGDPTHGQLAQAFNQLQHRPFRANIGGINKVRNEYHVYMTDAQG
                     KVLFDSANKAVGQDYSRWNDVWLTLRGQYGARSTLQNPADPESSVMYVAAPIMDGSRL
                     IGVLSVGKPNAAMAPVIKRSERRILWASAILLGIALVIGAGMVWWINRSIARLTRYAD
                     SVTDNKPVPLPDLGSSELRKLAQALESMRVKLEGKNYIEQYVYALTHELKSPLAAIRG
                     AAEILREGPPPEVVARFTDNILTQNARMQALVETLLRQARLENRQEVVLTAVDVAALF
                     RRVSEARTVQLAEKKITLHVTPTEVNVAAEPALLEQALGNLLDNAIDFTPESGCITLS
                     AEVEQEHVTLKVLDTGSGIPDYALSRIFERFYSLPRANGQKSSGLGLAFVSEVARLFN
                     GEVTLRNVQEGGVLASLRLHRHFT"
     gene            34213..35565
                     /gene="creD"
                     /locus_tag="NBI74_001774"
     CDS             34213..35565
                     /gene="creD"
                     /locus_tag="NBI74_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein CreD"
                     /protein_id="HCD1932131.1"
                     /translation="MLKSPLFWKMTTLFGAVLLLLIPIMLIRQVIVERADYRSDVEDA
                     IRQSTSGPQKLVGPLIAIPVTELYTVQEEDKTVERKRSFIHFWLPESLMVDGNQNVEE
                     RKIGIYTSQVWHSDLTLKADFDVSRLSELNAPNITLGKPFIVISVGDARGIGVVKAPE
                     VNGTALTIEPGTGLEQGGQGVHIPLPEGDWRKQNLKLNMALNLSGTGDLSVVPGGRNS
                     EMTLTSNWPHPSFLGDFLPAKREVSESGFQAQWQSSWFANNLGERFASGNDTGWENFP
                     AFSVAVTTPADQYQLTDRATKYAILLIALTFMAFFVFETLTAQRLHPMQYLLVGLSLV
                     MFYLLLLALSEHIGFTVAWIIASLIGALMNGIYLQAVLKGWCNSMLFTLALLLLDGVM
                     WGLLNSADSALLLGTSVLVVALAGMMFVTRNIDWYAFSLPKMKASKEVTSDDELRIWK
                     "
     gene            complement(35625..36341)
                     /gene="arcA"
                     /locus_tag="NBI74_001775"
     CDS             complement(35625..36341)
                     /gene="arcA"
                     /locus_tag="NBI74_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003621071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator ArcA"
                     /protein_id="HCD1932132.1"
                     /translation="MQTPHILIVEDELVTRNTLKSIFEAEGYDVFEATDGAEMHQILS
                     EYDINLVIMDINLPGKNGLLLARELREQANVALMFLTGRDNEVDKILGLEIGADDYIT
                     KPFNPRELTIRARNLLSRTMNLGTVSEERRSVESYKFNGWELDINSRSLIGPDGEQYK
                     LPRSEFRAMLHFCENPGKIQSRAELLKKMTGRELKPHDRTVDVTIRRIRKHFESTPDT
                     PEIIATIHGEGYRFCGDLED"
     gene            36437..36577
                     /locus_tag="NBI74_001776"
     CDS             36437..36577
                     /locus_tag="NBI74_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1932133.1"
                     /translation="MTKVRNCVLDALSINVNNIISLVVGTFPQDPTVSKTAVILTILT
                     AT"
BASE COUNT         8791 a         9207 c         9961 g         8760 t
ORIGIN      
        1 ttctgcggga agggatatca tttaattata aattaaatga atgtgatttc tgtttttcat
       61 taagtattcc taacaactta atgtagatat tttaaatgtc tccaggctat ttcaccaggt
      121 tatcatttga tgctaacctt ctgacacatg cagtggagtt gttgtgcagc aggagtatgc
      181 tgatatgaaa gtgaatgcta aggataattt attcgcttaa tctattaatt tgccggaaac
      241 atttaaggaa tgaatatcct gaataaacca tcataatttt tagacagtgg atgtggagga
      301 aatatgttgc caggatgctg caaaaatgga attgttatca gtaaaatacc tgttatgcaa
      361 gcagggttaa aagaggtcat gaggactcac ttccctgaat atgaaataat atccagcgcc
      421 tctgcggagg accttacctt attacaatta cgtcgttccg gattagtcat tgctgattta
      481 gccggtgaaa gtgaagatcc acgttctgtt tgtgaacatt attattcttt aatctcacaa
      541 tatcgggaaa ttcactgggt tttcatggtg tcacgctcct ggtattccca ggcagtagaa
      601 ctgctcatgt gccctacggc gacgttattg tctgatgttg aacccattga gaatctggtc
      661 aagaccgtac gttccggcaa tacgcacgca gagcgtatca gcgccatgct gacctctccg
      721 gcaatgactg aaactcatga ttttagctat cgctccgtca ttctcactct ttcagagcgc
      781 aaggtactgc ggctattagg taaaggatgg ggcatcaacc agatagcttc attgcttaag
      841 aaaagtaata aaactatcag cgcccaaaaa aacagtgcga tgcgtcgact ggcaattcac
      901 agcaacgctg aaatgtatgc atggataaat agcgcgcagg gtgcaagaga acttaacttg
      961 ccttctgttt atggagatgc cgcagaatgg aacacagccg aattaagaag agaaatgtcg
     1021 cactcataga aaaatgcgtc atgagtagta tcggtattga gagtttattc agaaagtttg
     1081 cgggtaaccc ttataagctc catacctata ccagtcagga gtcatttcag gatgccatga
     1141 cgcggatctc gtttgcggcg gtcatttttt ctttttctgc catgagaagt gagcgcagag
     1201 agggattatc ttgcctgact gaactggcga ttaagtttcc gcgtacccgg cgtttagtta
     1261 ttgcggatga tgatattgaa gctcggctga ttggttcatt gtcgccatca ccgctggacg
     1321 gtgtattaag taaagcgtca acgctggaga tttttcatca ggaactcttt ttgtcattaa
     1381 atggtgtacg tcaggcgacc gaccgactga acaatcagtg gtacattaac caaagccgga
     1441 cgctaagccc cacggagaga gaaatattgc gctttatgtc gcgtggctac tcaatgacac
     1501 aaattgccga gcagcttaaa cgcaatatca aaacgatccg tgcacataaa tttaatgtga
     1561 tgtcgaaact gggcgtcagt tctgatgcag ggttgttgga ggccgcagat attctgttat
     1621 gtatgcggca ttgcgaagca agtaatgtgt tgcatcccta ttaatccgca tgatgccggg
     1681 tttacttccc cggcagtgct ttcatttcag cgtacaatcg ccacattgct gcacatccgg
     1741 taagcgataa cgctggcagc aagtgcggcg caccagcagg ccatcgcgca gtaccacggt
     1801 acgccagagt ggattatctt caccgttcgt gagcgttttc tcaaaaaaga gggcatggcg
     1861 cagcgattca accgtcgcct cgccgagcag ctgcttcatc tcagtgagat accagttgat
     1921 caaataaccg gtattactcc agataagttt gccgttgatc tctccggtcg cttctagtgc
     1981 ctgcacaacc ggaaccagcg cctggctgat taacgtttcc attcgctgct gcggcgaatg
     2041 tggtgttgcg tttttatctt cacacacatc gacccagaaa caggcgacgc gtccggtttc
     2101 gtgaaactca gcatggaaat gttccggcga cacatctaat gccttttcct gcgtcagtag
     2161 cgccagcatt aatggtggca ccatcaggcc gatataccat tgtgcccata gtgagatcag
     2221 cggtttgttc tcgcggatca tcgttggttg gttgcgatag atatgatcgg aatagaccgc
     2281 gagtagagaa cttagcgcat tcggtgatga ccattgcgcc agcgtcatgg cgttaagcgg
     2341 agcgggttca tccaggcgga taaactcgag caaatgttca cgatgtttgg cgatcgtcgc
     2401 acgtacggct tgtgcaagcc cagcatcctg cggctggaga tgcgttcgcc agatgatatc
     2461 ttcatagagc ggtgcggaac gataggccat aatcgggata gtaatctaaa tgataatgat
     2521 tgctaatcct agcgataggt ttacccgata gcaagggatt tctctggctt gcaaatgata
     2581 aaaattatca tatgatattg gttatcatta tcaatgaaag agatgaaacc atgttgcaac
     2641 gtacgctggg cagtggctgg ggagtgttgc tgccgggatt gctgattgca gggctgatgt
     2701 atgcggattt atcgccggat cagtggcgga ttgtcattct gatgggattg atattgacgc
     2761 cggtgatgct gtatcacaaa cagttacggc attacatttt gctaccatcg tgcctggcgc
     2821 ttagtgctgg catcatgctg atgctaatga atttgaatca gggatgaaaa atcaaggaag
     2881 aaacaagaaa ggaagtaaag ataattggtg cgaggggggg gacttgaacc cccacgtccg
     2941 taaggacact aacacctgaa gctagcgcgt ctaccaattc cgccaccttc gcataccatc
     3001 aattcttaaa aagaattgct accacggagg cgcattctag tggttttcag cttttcgtca
     3061 atagttaatt atcgccggag gtgtaattgc tggaaaaatg tccatcagga aactaacgtg
     3121 caggtttggt agttttgtag gtcggataag gcgttcacgc cgcatccgac acggtatttg
     3181 ggtgcagatg ccagatgcga cgcgggcgcg tcttatctgg cctacgaagg gctaacgtgc
     3241 aggttttgta ggtcggataa ggcgttcacg ccgcatccga cacggtattc ggcgagataa
     3301 ttaacctttc ttcgcctggc gggtcataat ggcgcgatac accttgaaac gcccggtttg
     3361 cgcaatcact tcatggaagc caaatgtctc atccagcacg tccgggtaag gcaggaaggc
     3421 gttcgctaca attcgcagct cgccgccgct attaagatga cgcaccgcgc cgcgaatcag
     3481 cgtttgcgcc gcatccaggc tggtttgcat cccatcgtgg aacggcgggt tggagatgat
     3541 catatcaaaa cgacctttca cctcggaaaa gacgttgctg gcaaagactt caccttcaac
     3601 accgttggcc gcaagtgttg cgcggctggc ttctaccgct ggcgcagaga catcgcacaa
     3661 ggtgagacga attttcggcg aatggcgcgc aaaggcaact gaaagcacac ccgcgccaca
     3721 gccgacatcc agcactttac ctttcgtgtg cggggttaac gtcgagagca gcaactggct
     3781 accgacatcc agaccgtcgc ggctaaacac gccaggcagc gttttgaccg tcaggccatc
     3841 gacgctgtat tcgccccaga atttatcggc atcaaatacc ggctgttttt ccagacgacc
     3901 aaaatagagg ccacagcgac gagcgctgtc gactttattc aacggcgcat aatctgccag
     3961 catctgctcg gcgctgcgca cgccgctgcg gttctcgcca acgacaaaaa tatctgtccc
     4021 cactggcagc agagaaagta aattcatcaa ctggaactgg gcttccggtt tgttcttcgg
     4081 ccagtagtaa atcagcgtat cgcaatctgc gacgtcatcc accgtggcga ccagactgaa
     4141 acgggcgtta tcccccatct ggcggcttaa tacctgccag tggtggaatt gctgggtatg
     4201 agcacggctg gccgcggtat ctaaacgcgc gggcaggtca tcctgtaagt ctccggcaaa
     4261 cagaatacgg ctttgttcga aatcatcact gtgacgcagc aagacttcac ttgccggggt
     4321 aaatgcagac atggaatgct cctcaattga tactggcggc gattatagcc atatgttggc
     4381 gcggtatcga cgaatttgct atatttgcgc ccctgacaac aggagcgatt cgctatgaca
     4441 tcccgacgag actggcagtt acagcaactg ggcattaccc agtggtcgct gcgtcgccct
     4501 ggcgcgttgc agggggagat tgccattgcg atcccggcac acgtccgtct ggtgatggtg
     4561 gcaaacgatc ttcccgccct gaccgatcct ttagtgagcg atgttctgcg cgcattaacc
     4621 gtcagccccg atcaagtgct gcaactgacg ccagaaaaaa tcgcgatgct gccgcaaggc
     4681 agtcgctgca acagctggcg gttgggtact gacgaaccgc tatcactgga aggcgctcag
     4741 gtggcatcac cggcgctcac cgaattacgg gcaaacccaa cggcacgcgc cgcgttatgg
     4801 caacaaattt gcacatatga acacgatttc ttccctcgaa acgactgatt taccggcggc
     4861 ttaccacatt gaacaacgcg cccacgcctt tccgtggagt gaaaaaacct ttgccagcaa
     4921 ccagggcgag cgttatctca actttcagtt aacgcaaaac ggcaaaatgg cggcgtttgc
     4981 gattacgcaa gtggtgctgg atgaagctac attgttcaat attgcggtcg atcctgacta
     5041 tcagcgtcag ggattgggaa gggtgctgct ggaacatctg atcgacgaac tggaaaaacg
     5101 cggcgtggcg acactatggc tggaagtccg tgcctcaaat gctgccgcca ttgccctgta
     5161 cgaaagttta ggctttaacg aggcgacgat tcgccgcaat tactacccca ccacggacgg
     5221 tcgcgaagac gccatcatca tggcgttgcc aatcagtatg taagacaagg tggaataatg
     5281 aagtgggact ggattttctt tgatgccgat gaaacgctgt ttacctttga ctcattcacc
     5341 ggcctgcagc ggatgtttct tgattacagc gtcaccttta ccgctgaaga ttttcaggac
     5401 tatcaggccg ttaacaagcc tctgtgggtg gattatcaaa acggcgcgat cacttcatta
     5461 cagcttcagc acgggcgttt cgagagctgg gccgaacggc tgaacgtcga gccgggtaaa
     5521 ctcaacgaag cctttattaa tgcgatggcg gaaatctgca cgccgctgcc gggcgcggtt
     5581 tctctgctta acgccattcg tggcaacgcc aaaatcggca tcatcaccaa cggctttagt
     5641 gccttgcaac aggtgcgtct ggaacgcacg ggcctgcgtg attacttcga tttgctggtg
     5701 atttccgaag aagttggcgt tgccaaaccg aataagaaaa ttttcgatta tgcgctggaa
     5761 caggcgggca atcctgaccg ttcacgcgtg ctgatggttg gcgacactgc cgagtccgat
     5821 attctcggtg gcatcaacgc cgggcttgcg acctgctggc tgaatgcgca caatcgcgag
     5881 caaccagaag gcatcgcgcc tacctggacc gtttcttcgt tgcacgaact ggagcagctc
     5941 ctgtgtaaac actgattgcc tcccccccgt tgatgggtaa aatagccgca atttttcgtt
     6001 ttcaacaagc gcggcgcgat gccgcttact caagaagaaa gaattatgac gttgtctcct
     6061 tatttgcaag aggtggcgaa gcgccgcact tttgccatta tttctcaccc ggacgccggt
     6121 aagactacca tcaccgagaa ggtgctgctg ttcggacagg ccattcagac cgccggtaca
     6181 gtaaaaggcc gtggctctaa ccagcacgct aagtcggact ggatggagat ggaaaagcag
     6241 cgtgggatct ccattactac gtctgtgatg cagtttccgt atcacgattg cctggttaac
     6301 ctgctcgaca ccccggggca cgaagacttc tcggaagata cctatcgtac cctgacggcg
     6361 gtggactgct gtctgatggt tatcgacgcc gcaaaaggtg ttgaagatcg tacccgcaag
     6421 ctgatggaag ttacccgtct gcgcgacacg ccgatcctca cctttatgaa caaacttgac
     6481 cgtgatatcc gcgacccgat ggaactgctc gatgaagttg agaacgagct gaaaatcggc
     6541 tgcgcaccga tcacctggcc gatcggctgc ggcaagctgt ttaaaggcgt ttaccacctt
     6601 tataaagacg aaacctatct ctatcagagc ggtaaaggcc acaccattca ggaagtccgc
     6661 attgttaaag ggctgaataa cccggatctc gatgcagcgg tcggtgaaga tctggcacag
     6721 cagctgcgtg acgaactgga actggtgaaa ggcgcgtcta atgagttcga caaagagctg
     6781 ttccttgcgg gcgaaatcac tccggtattc ttcggtactg cgctgggtaa cttcggtgtc
     6841 gatcatatgc tggatggcct ggtggagtgg gctcctgcgc cgatgccgcg tcagactgat
     6901 acccgtaccg tagaggcgag cgaagacaaa tttaccggct tcgtatttaa aattcaggcc
     6961 aacatggacc cgaaacaccg cgaccgcgtg gcgtttatgc gcgtggtatc cggtaaatat
     7021 gaaaaaggca tgaagctgcg ccaggtgcgc actgcgaaag atgtggtgat ctccgacgcg
     7081 ctgaccttta tggcaggtga ccgttcgcac gttgaagaag catatccggg cgatatcctc
     7141 ggcctgcaca accacggcac cattcagatt ggcgacacct ttacccaggg tgagatgatg
     7201 aagttcaccg gtattccgaa cttcgcgcca gaactgttcc gtcgcatccg cctgaaagat
     7261 ccgctgaagc aaaaacagct gctcaaaggg ctggtacagc tttccgaaga gggcgcggtg
     7321 caggtgttcc gtccaatctc caacaacgat ctgatcgttg gtgcggttgg tgtgctgcag
     7381 tttgatgtgg tggtatcgcg cctgaagagc gaatacaacg ttgaagcagt atatgaatca
     7441 gtcaacgttg ccaccgcccg ctgggtagaa tgtgcagacg cgaagaaatt cgaagagttc
     7501 aagcgtaaga acgaaagcca actggcgctt gatggcggcg ataacctcgc ttacatcgct
     7561 accagcatgg tcaacctgcg cctggcgcag gaacgttatc cggacgttca gttccaccag
     7621 acccgcgagc attaattctt gcctccaggg cgcggtagcc gctgcgccct gtcaatttcc
     7681 cttccttatt agccgcttac ggaatgttct taaaacattc acttttgctt atgttttcgc
     7741 tgatatcccg agcggtttca aaattgtgat ctatatttaa caaagtaatg acatttctga
     7801 cggcgttaaa taccgttcaa tgcgtagata tcagtatcta aagccgtcga ttgtcattct
     7861 accgatatta ataactgatt cagaggctgt aatggtcgtt attcatcacc catcgctttt
     7921 gtgatggcga ccattgactt ctgtagaggg tgaagtctct ccctattcag caatgcaacc
     7981 tcgtgttgcc aggctcaaat tacgagcaaa cttacaggaa taaatcgatg actatgacaa
     8041 gactgaagat ttcgaaaact ctgctggctg taatgttgac ctctgccgtc gcgacaggct
     8101 ctgcctacgc ggaaaacaac gcgcagacta ccaatgaaag cgcagggcaa aaagtcgata
     8161 gctctatgaa taaagtcggt aatttcatgg atgacagcgc catcaccgcg aaagtgaagg
     8221 cggccctggt ggatcatgac aacatcaaga gcaccgatat ctctgtcaaa accgatcaaa
     8281 aagtcgtgac cctgagcggt ttcgttgaaa gccaggctca ggccgaagag gcagtgaaag
     8341 tggcgaaagg cgttgaaggg gtgacctctg tcagcgacaa actgcacgtt cgcgacgcta
     8401 aagaaggctc ggtgaagggc tacgcgggtg acaccgccac caccagtgaa atcaaagcca
     8461 aactgctggc ggacgatatc gtcccttccc gtcatgtgaa agttgaaacc accgatggcg
     8521 tggttcagct ctccggtacc gtagattctc aggcacaaag tgaccgtgct gaaagtatcg
     8581 ccaaagcggt agatggtgtg aaaagcgtta aaaatgatct gaaaactaag taattcgtcg
     8641 taattcgtcc tcccgaactt tgtcgggagg cgcgatgtgc accacactaa aaatatcgca
     8701 ttgagtgatt ttcacgctca tatcaagcgg tgacattaac tatggtaaag gagacgctta
     8761 tgtttcgttg gggcatcata tttctggtta tcgcgttaat cgccgccgca cttgggtttg
     8821 gtggtctggc cggtaccgct gcaggcgcag ctaaaattgt ctttgtcgtc gggattattc
     8881 tgttcctggt gagtttgttc atgggccgaa aacgacccta gatttcagac cattaagaaa
     8941 attttatcca aagccagtcc agcggactgg cttttgcggt tttagcgaat aataaattgc
     9001 gttactttgt cattctttca caacaaaaac aggaaggcag aggtggggca gcgaatacct
     9061 gtaacgcttg gtaatattgc gccgttgtcg ctaaggccgt tccagcctgg acgaatagct
     9121 ctggtgtgcg aaggcggcgg acagcgtgga attttcacgg ctggcgtgct ggatgagttt
     9181 atgcgcgcgc agtttaatcc tttcgatctt tatctcggca catctgccgg ggcgcagaac
     9241 ctctcggcgt ttatctgcaa tcagcccggt tacgcgcgca aagtcatcat gcgctatacc
     9301 acaaaacgcg aatttttcga tccattgcgc tttgtccgtg gaggaaatct tatcgatctc
     9361 gactggctgg tggaggccac cgcaagccag atgccgttgc aaatggatac cgccgcgcgg
     9421 ttgtttgaca gcggcaaatc gttttatatg tgcgcctgtc gtcaggatga ctacgcgcct
     9481 aattactttt taccaaccaa acaaaactgg ctggatgtga ttcgcgcctc cagtgcgata
     9541 cctggctttt atcgtagcgg agtgtcgctg gaaggcatta actacctgga tggcgggatc
     9601 agtgatgcga ttccggttaa agaggcggca aggcagggcg ctaaaacgtt ggtcgtcatc
     9661 cgcactgtgc cgtcacaaat gtactacacg ccgcagtggt tcaaacgcat ggaacgctgg
     9721 ctgggtgaca gtagcctgca gccgctggtc aatctggtgc agcatcatga aaccagctat
     9781 cgtgacattc agcaatttat tgagaaacca ccgggcaagc tgcggatatt cgaaatttat
     9841 ccaccgaaac cattacatag tatcgcgctt ggcagtcgga ttccggcgct gcgtgaagac
     9901 tataaacttg ggcgtttatg cggtcgttat ttcctcgcca cggttggcaa gctattaact
     9961 gaaaaagcgc cgcttacccg ccatctggtg ccagtggtga cgccggaatc gatcgttatt
    10021 ccgcctgcgc cagtcgccaa cgatacgctg gttgccgaag tgagcgatgc tccgcaggcg
    10081 aacgacccga catttaacaa tgaggatctg gcttgatttg ccgttttatc gatacccact
    10141 gccattttga tttcccgccg tttagtggcg atgaagaggc cagcctgcaa cgcgcggcac
    10201 aagcgggcgt aggcaagatc attgttccgg cgacagaggc ggaaaatttt gcccgcgtgc
    10261 aggcgttagc ggaaaaatat caaccgctgt atgccgcatt ggggttgcat ccggggatgt
    10321 tggaaaaaca tagcgatgtg tctcttgatc agctacagca ggcgctggaa aggcgtccgg
    10381 cgaaggtggt ggcggtgggg gagatcggtc tggatctctt cggcgacgat ccgcaatttg
    10441 agaggcagca gtggttactc gacgaacagc tgaaactggc gaaacgctac gatctgccgg
    10501 tgatccttca ttcccggcgt acacacgaca aactggcgat gcatcttaaa cgtcacgatt
    10561 taccgtgcac tggcgtggtt cacggttttt ccggcagcct gcaacaggcc gagcggtttg
    10621 tacagctggg ctacaaaatc ggcgtaggcg gtactatcac ctatccacgc gccagtaaaa
    10681 cccgcgatgt catcgcaaaa ttaccgctgg catcgttgtt gctggaaacc gacgcgccgg
    10741 atatgccgct caacggcttt caggggcagc ctaaccgacc ggaacaggct gtgcgggtgt
    10801 ttgacgtgct ttgcgagttg cgcccggaac cagaagatga gattgccgaa gtgttgctta
    10861 ataatacgta tgcgttgttt agcgttagtg ggtagtttac ccttcgtagg cagcatccgg
    10921 cagtcggcgc ataatgcctg atgcgacgct tgtcgcgtct tatcatgcct acaggtttgt
    10981 gccgaaccgt aggccggata aggcgttcac gccgcatccg gcagttggcg cacaatgcct
    11041 gatgcgacgc ttgacgcgtc ttatcatgcc tacaagtctg tgccgaaccg taggccggat
    11101 aaggcgttta cgccgcatcc ggcagttggc gcacaatgcc tgatgcgacg cttgtcgcgt
    11161 cttatcatgc ctacaagtct gtgccgaacc gtaggccgga taaggcgttc acgccgcatc
    11221 cggcagtcgg cgcataatgc ctgatgcgac gcttgacgcg tcttatcatg cctacaggtt
    11281 tgtgccgaac cgtaggccgg ataaggcgtt cacgccgcat ccggcagtcg gcgcataatg
    11341 cctgatgcga cgcttgtcgc gtcttatcat gcctacaagt ctgtgccgaa ccgtaggccg
    11401 tatccggcat gtcacaaata gagcgccgga aatatcaacc ggctcacccc gcgcaccttt
    11461 aacgcatcag ccaacggctc aacgtcttcc ggcgtggcgc tcgcccagct ttgcgcctcg
    11521 ccatacacgc cgtgggcatg aaacgcgttc aggcgtaccg gaacatcgcc aagtcccttg
    11581 ataaacaccg ccagttcttc gatgtgttgc aaataatcca cctggccagg gattaccagc
    11641 aaacgcagtt ccgccagctt accgcgctct gccagcaaac agatgctgcg cttaatctgc
    11701 tgattatcgc gtccggttaa ttgttgatga cattcgctcc cccacgcttt gagatcgagc
    11761 attgcgccgt cacacaccgg gagtaatttt tcccagccgg tttcgctcaa cataccgtta
    11821 ctgtccacga ggcaggtgag gtggcgcagt tgcggatcgt ttttgatggc tgtaaacagc
    11881 gccaccacaa acggcagctg ggtcgtggct tcgccgccgc ttaccgttat cccttcgata
    11941 aacagcaccg ctttgcggat atggctaagc acctcatcca cgctcatgga ttgcgccatt
    12001 ggcgtggcat gttgcggaca catcttcagg caggtatcac actgctcgca gaccacagcg
    12061 ttccacagca ctttgccgtc aacaatctgc aacgcctgat gcggacactg tggcacgcac
    12121 tctccgcaat cattgcagcg tcccatcgtc cacggattgt gacagttttt gcagcgcaga
    12181 ttgcagccct gcaaaaacag agccagacga ctgcctggcc cgtcaacgca ggagaagggg
    12241 ataatcttac tgactaaagc gcatctgctg ttcatggctt atcacgcgcg gctggcgttc
    12301 cagaatacga gtgttgcgtg cggcttcttc tcccagccag gtggtattgg tacgtgaacc
    12361 ttcggcacga tatttttcta aatccgacaa acgcaccata taaccggtaa cgcgaaccag
    12421 atcgttaccg ctgacattgg cggtaaattc acgcattccg gctttaaagg caccgaggca
    12481 aagctgtacc agcgcctgcg gattacgttt gatggtttcg tcgagcgtca gaatgtcgct
    12541 gattccagag taataataag catgatgcgg ggcgacggtt tgcagatggg taatcggatc
    12601 tggctcatca ccatacggca agcgtgcgcc cggcgtggtg ccgatatcgg aacttatccc
    12661 cgactgtgcg tgtagcatgg cgcgattttg ccagccatat ttcacggggg tattggcgac
    12721 aaactccgcc agttgcgcgc taatgcgata acccacttca ttcgcagcgg cttctttacc
    12781 gtaacgtgcg gcaattcctt ctttctcaca caacaagtta accgcttctg ccagtccgta
    12841 catgccaaac attggcacaa aacgttcagg gttaatcagc ccttctttca ccaggaagct
    12901 attctcaaag aagtgtgatt gttgatagag gaattcacac cgcgcatcga tgatggcgat
    12961 ttgctgctgg cagtagtgcg gtagagtgcg cgtaaagaag tcatccagcg attcactgcg
    13021 ctcggcaatg gctttcaggt tgaggcgtac cagcgtactg ccaccacccg ctaacggcag
    13081 tgagttgtaa cagctcacaa tcccgtagcc cccatttgtg aaaattttat catgcacggg
    13141 accgttggcg atgtgcggtt tgctacattc acagatgttt ttcgccactt ccagcagcag
    13201 gtcatcaggg gtgatttcag gatcgtagat aaaagtcagg ttcggtgaaa cctgcttcag
    13261 ctctgcgtct gcgcgtaaaa tcgcacgggt aatgggtgaa tcagatgggc caatattggc
    13321 gtgcataaag gcatctggca gggttctgtc gaggtaacgc cagaaacgtt ttattcgaat
    13381 atcgatctcg ttttgtgtta gaattctaac atacggttgc aacaacgcat ccagttgccc
    13441 caggtagacc ggcatcgatg tgaccgaagg aacgtggtgg taaagaatgg tcagcagaga
    13501 gagtgcgtca tcaagatctt ttgcgccttc cagctccagc cattctgaac cgttggccaa
    13561 aaaacgggcg taatcgggta agacatagcg cggtttatac ggcgcatgac cttcaaacat
    13621 atcgcagatt acaccttcat ccagcgcgcg gcgggcttcg gcaggaagct gtgggtaagg
    13681 cagattgttt tctgcttcca gcgccagaaa atggcgcttc tgctccgggc taagtaccgg
    13741 actggtgaca atttgctggc aacgttgttg cagtgcattt tcatgagaag tgggcatctt
    13801 ctgttccttt ttatgccgaa ggtgatgcgc cattgtaaga agtttcgtga tgttcacttt
    13861 gatcctgatg cgtttgccac cactgacgca ttcatttgaa agtgaattat ttgaaccaga
    13921 tcgcattaca gtaatgcaat tttgtaagta gatttcctta attgtgatgt atatcgaagt
    13981 gtgttgcgga gtcgatgtta gaatactaac aaactcgcaa ggtgaatttt attggcgaca
    14041 agccaggaga atgaaatgac tgatctgaaa gcaagcagcc tgcgtgcact gaaattgatg
    14101 gacctgacca ccctgaatga cgacgatacc gacgagaaag tgatcgccct gtgtcatcag
    14161 gccaaaactc cggtcggtaa taccgccgct atctgtatct atcctcgctt tatcccgatt
    14221 gctcgcaaga cgctgaaaga gcagggtacc ccggaaatcc gtatcgctac ggtaactaac
    14281 ttcccacacg gtaacgacga catcgaaatc gcgctggcag aaacccgtgc ggcaatcgcc
    14341 tacggtgccg atgaagttga tgttgtgttc ccgtaccgtg cactgatggc gggtaacgag
    14401 caggttggtt ttgatctggt gaaagcctgt aaagaggctt gcgcagcagc gaacgtactg
    14461 ctgaaagtga ttattgaaac cggcgaactg aaagacgaag cgctgatacg caaagcgtct
    14521 gaaatctcca ttaaagcggg tgctgatttc atcaaaacct ctaccggtaa agttgcagtg
    14581 aacgcgacgc cggaaagcgc gcgcatcatg atggaagtga tccgtgatat gggcgtagag
    14641 aaaaccgttg gtttcaaacc tgcgggcggc gtgcgtactg cggaagatgc gcagaaatat
    14701 ctcgcgattg cagatgaact gttcggtgct gactgggcag atgcgcgtca ctaccgcttt
    14761 ggtgcttcca gcctgctggc aagcctgctg aaagcgctgg gccacggcga cggtaagagc
    14821 gccagcagct actaagctgc cttttgccag agggtggatg ccggatgcgg cgcgaacgcc
    14881 ttatccggcc tacgggacga ttgccgtgta ggcaggataa gaagcggtca gcatcaggca
    14941 ataacaacat ccgccatctg gcaacaacgc cttcaaactt attccacggg aggttacctt
    15001 gtttctcgca caagaaatta ttcgtaaaaa acgtgatggt catgcgctga gcgatgaaga
    15061 aattcgcttc tttatcaacg gcattcgcga caacactatc tccgaagggc agattgccgc
    15121 cctcgcgatg accattttct tccacgatat gacaatgccg gagcgtgtct cgctgacgat
    15181 ggcgatgcga gattcaggaa ccgttctcga ctggaaaagc ctacatctga atggcccgat
    15241 tgttgataaa cactcgaccg gcggcgtcgg cgatgtgact tcgctgatgt tggggccaat
    15301 ggtcgctgcc tgcggcggct atattccgat gatctccggt cgcggcctcg gccatactgg
    15361 tggtacgctc gacaaactgg aatccatccc tggcttcgac atcttcccag atgacaaccg
    15421 tttccgcgaa attattaaag atgttggcgt ggcgattatt ggccagacca gttcactggc
    15481 tccggcggat aaacgtttct acgcgacccg cgatattacc gcaaccgtgg attccatccc
    15541 gctgatcact gcctcgatcc tggcgaagaa actggcggaa ggtctggatg cactggtgat
    15601 ggacgtgaaa gtcggtagcg gtgcgtttat gccgacctac gaactctctg aagcccttgc
    15661 cgaagcgatt gttggcgtgg ctaacggcgc tggcgtgcgc accaccgcgc tactcaccga
    15721 catgaatcag gtgctggcct ccagtgccgg taacgcggtt gaagtacgtg aagcggtgca
    15781 gttcctgact ggtgagtacc gtaacccgcg tctgtttgat gtcacgatgg cgttgtgtgt
    15841 ggagatgctt acctccggca aactggcgaa agatgacgcc gaagcgcgcg cgaaattgca
    15901 ggcggtactg gacaacggta aagcggcaga agtctttggt cgtatggtag cggcacaaaa
    15961 aggcccgacc gacttcgttg agaactacgc gaagtatctg ccgacagcga tgctgacgaa
    16021 agcagtctat gctgataccg aaggttttgt cagtgaaatg gatacccgcg cgctgggaat
    16081 ggcagtggtt gcaatgggcg gcggtcgccg tcaggcatct gacaccatcg attacagcgt
    16141 cggctttact tatatggcgc gtctgggcga ccaggtagac ggtcagcgtc cgctggcggt
    16201 tatccacgcg aaagacgaaa acagctggca ggaagcggcg aaagcggtga aagcggcaat
    16261 taaacttgcc gataaagcac cggaaagcac accaactgtc tatcgccgta tcagcgaata
    16321 acggtatact gatctgatca tttaaatttg aagcactgag tacggagaac atatgaaacg
    16381 tgcatttatt atggtgctgg actcattcgg catcggcgct acagaagatg cagaacgctt
    16441 tggtgacgtc ggggctgaca ccctgggtca tatcgcagaa gcttgtgcca aaggcgaagc
    16501 tgataatggt cgtaaaggcc cgctcaatct gccaaatctg acccgtctgg ggttggcgaa
    16561 agcacacgaa ggttctaccg gtttcattcc ggcgggaatg gacggcaacg ctgaagttat
    16621 cggcgcgtac gcatgggcgc acgaaatgtc atccggtaaa gacaccccgt ctggtcactg
    16681 ggaaatcgcc ggtgtcccgg ttctgtttga gtggggatac ttctccgatc acgaaaacag
    16741 cttcccgcaa gagctactgg ataaactggt cgagcgcgct aatctgccgg gttacctcgg
    16801 taactgccac tcttccggta cggtcattct ggatcagctg ggcgaagagc acatgaaaac
    16861 cggcaagccg attttctata cctccgctga ctcagtgttc cagattgctt gccacgaaga
    16921 aacctttggc ctggataaac tctacgaact gtgcgaaatc gcccgtgaag agctgaccaa
    16981 cggcggctac aatatcggtc gtgttatcgc tcgtccgttt atcggcgaca aagccggtaa
    17041 cttccagcgt accggtaacc gtcatgacct ggctgttgaa ccgccagcac cgaccgtgct
    17101 gcagaaactg gttgatgaaa aacacggcca ggtggtttct gtcggtaaaa ttgcagacat
    17161 ctacgctaac tgcggcatca ccaaaaaagt gaaagcgacc ggcctggacg cgctgtttga
    17221 cgccaccatc aaagagatga aagaagcggg tgataacacc atcgtcttca ccaacttcgt
    17281 tgacttcgac tcttcctggg gccaccgtcg cgacgtcgcc ggttatgctg cgggtctgga
    17341 actgttcgac cgccgcctgc cagagctgat gtctttgctg cgcgatgacg acatcctgat
    17401 cctcactgct gaccatggtt gtgatccgac ctggaccggt actgaccata cccgtgaaca
    17461 cattccggta ctggtttacg gcccgaaagt aaaaccgggt tcgctggggc accgtgaaac
    17521 tttcgcggat atcggccaga ctctggcaaa atattttggt acttctgata tggaatatgg
    17581 caaagccatg ttctgatgga tttgggcgga gcagtgactc cgcctttaat atgtcacaaa
    17641 aaggataaaa tgatggctac tccacacatt aatgcagaaa tgggcgattt cgctgacgta
    17701 gttttgatgc caggcgaccc gctgcgtgcg aagtatattg ctgaaacttt ccttgaagat
    17761 gctcgtgaag tgaacaacgt tcgcggtatg ctgggcttca ccggtactta caaaggccgc
    17821 aaaatttctg taatgggcca cggtatgggt atcccgtcct gctccatcta cactaaagaa
    17881 ctgatcaccg atttcggcgt gaagaaaatc attcgcgttg gttcctgtgg cgcagttctg
    17941 ccgcacgtaa aactgcgtga cgtagtgatt ggtatgggtg cctgcaccga ttccaaagtt
    18001 aaccgcatcc gttttaaaga ccatgacttt gcagctatcg ctgacttcga catggtgcgt
    18061 aacgcagtag atgcagctaa agcactgggc attgatgctc gcgttggtaa cctgttctcc
    18121 gctgacctgt tctactctcc ggacggtgaa atgttcgacg tgatggaaaa atacggcatt
    18181 ctcggcgtgg aaatggaagc agctggtatc tacggcgtgg ctgcagagtt tggcgcgaaa
    18241 gccctgacca tctgcaccgt atctgaccat attcgcacgc acgagcagac cactgccgct
    18301 gagcgtcaga ctaccttcaa cgacatgatc aaaatcgcac tggaatccgt tctgctgggc
    18361 gataaagagt aattgtgttt cgctgaaagg cgattgtcgt gtgaagccgg agcgggagac
    18421 tgctccggct ttttagtatt tattcatcaa tagcaactga tttatagatt tactattatt
    18481 tttcatatgg atagaaaatt aaatccattc gagtatctat tcacgaatct attcatttat
    18541 gttcagccgt catccagtaa gggcatatca gcaactctgg cggacaacca taaattcctg
    18601 ccattttgtt tttgatgttt gaactaagcc gttccgtctt ttcgatttgt tttagagaat
    18661 taatcgagat acccaacatt ttactaactt cagcaagtga tagtttatgc ctgatacgca
    18721 cggcggttag ccaggatata ccctggctga acattaaatt gataatctcc cgttcctggt
    18781 tcttcagatc gatcatattt ccttccctgg atattttaat cagcgagtta ccgcaccgcc
    18841 cctgccatcc acgccgataa ttcccttagc tctttttcct gttcagggaa gtcgaccaac
    18901 agagcttcac actcttgttg cagcatatcc gcgcggtaca ggcagccttg cagtcgtccg
    18961 gcgagagctt ccagaggtgc ggggttgagg ctatcggtaa acacctgggc gcgggtgata
    19021 tggccttttt cgacgtcgaa atgcagttct acgccgcccc aggtaaagcg ttcatccagc
    19081 agatgcgaga acgctggagc ctgtccgaaa ttccattccc agctactctg gcgggcaaag
    19141 gtttcggcga agttcggcaa gtccggcgtt ttgtccgggg agataatttc cgcttctaca
    19201 cgctcgccat aatgggcgaa aaaggcttcg gatatggcct cgcaaacctg ctcatgggta
    19261 attcccggca gcaattcggt cagattggtc acgcgggaac gtaccgacgt aatgcctttc
    19321 gccgccagtt tctttttatc cgggttgaga tagtttgcca ggcggctaag gtcggcattg
    19381 agtagcaagg tgccgtgatg gaagccacga tctttggttt cgcgataggc cgagcctgag
    19441 actttacggt cgccttcggc ggttttcacc accaaatcgt tacgtccgga cgcttcggcg
    19501 ctgacaccga gcgcgttcag tgcattgagc acaatcgagg tggagatcgt tttatcgtat
    19561 tccggcttgc cagccatgaa ggtaaaacag gtattgccga gatcgtggaa caccgcgccg
    19621 ccaccgctac tgcgccgtgc caggcgaacg ttatcttctt ccatccgccg ggtattacac
    19681 tctttccacg ggttctgcgc gcgaccaatc actaccgtgt cggcattgcg ccagagaaac
    19741 aaaacgcgct gcgtggcggg catctggcga aaaatacact cctctaccgc caggttaaac
    19801 cacgggtcgt aagagtcaga gatgagcagg cgtaatgtgg acataacgat ttcctttctt
    19861 gtagtaatgg gtcactcttt tttctcgctt tcttcctctt ccggcaccgg tttgctggcg
    19921 gttaacagga agggggattg ctgccagcgg gtgcgtttac cctgtagcag cgtgcgggtc
    19981 agcactacgc cgattgccag cgagagcaac aacatcaggc gtaaaatgtt agtggtgtta
    20041 tccacctgtt gagcttcggt ggcgagcgta tgggtgtcga gcgtcaggcg cagatagcca
    20101 agcggaccat ttttccctgc aatgggttcg acaatctgct ggttaaaata gccgcccgct
    20161 tttttaccgt cgagcgccag ccggtcacgc acctcgacgc tttcgccaga acgggcgata
    20221 agatcgcctt gttcgtcata cacgcccgcg tcgaggatgc ggctttcatc cgttaactga
    20281 tcgaggatcg cctgaatacg tttttcatcc ggcgagtcgg tacgcatcag cggcgcaacg
    20341 ttcagcgtca cctgccgcgc cagggtgcgg gccagttctt ccagctgcgg attacgctgt
    20401 cgctggtggt tttgactaaa ccatgatgcc ccctgcatca gcgccactaa caaggcaaga
    20461 cagaacagga caatcactgc ccgatgcagc cggaatttca gttttgtgcg agccatcttc
    20521 caccctttga aaatttgaga cttaatgttg ccagaagcaa tggatacaag gtagcctcat
    20581 gcgttatttt ccctgcttcg aacgatttta caggagcctt aatgcctaac attacctggt
    20641 gcgacctgcc tgaagatgtc tctttatggc ctggtctgcc tctttcatta agtggtgatg
    20701 aagtaatgcc actggattac cacgcaggtc gtagcggctg gctgctgtat ggtcgtgggc
    20761 tggataaaca acgtctgacc caataccaga gcaaactggg cgcggcgatg gtgattgttg
    20821 ccgcctggtg cgtggaagat tatcaggtga ttcgtctggc aggttcgctt accgcccggg
    20881 ctacgcgcct ggcccatgaa gcacaactgg atgtcgcgcc gctggggaaa attccgcatc
    20941 tgcgcacgcc aggtctgctg gtgatggaca tggattccac cgccattcag attgaatgta
    21001 ttgacgaaat tgccaaactg gccggaaccg gcgagatggt ggcggaagta accgagcggg
    21061 cgatgcgcgg cgaactcgat tttaccgcca gcctgcgcag ccgcgtggcg acgctgaaag
    21121 gcgctgacgc gaacattctg caacaggtgc gggaaaatct gccgctgatg ccaggcttaa
    21181 cgcaactggt actcaagctg gaaacgctgg gctggaaagt ggcgattgcc tccggcggtt
    21241 ttaccttctt tgccgaatac ctgcgcgata agctgcgcct gaccgccgta gtagccaatg
    21301 aactggagat catggacggt aaatttaccg gtaatgtgat tggcgacatt gtagacgctc
    21361 agtataaagc gaaaaccctg acccgcctgg cgcaggagta tgaaatcccg ctggcgcaaa
    21421 ccgtggcgat tggcgatggt gccaacgacc tgccgatgat caaagcggca ggtctgggga
    21481 ttgcctacca tgccaagcca aaagtgaatg aaaagacgga agtcaccatc cgtcacgctg
    21541 acctgatggg ggtattctgc atcctctctg gcagcctgaa tcagaagtaa ttgtccgccc
    21601 gtcatcctgc gggcggcaca gtattaacga ggtacaccgt ggcaaaagct ccaaaacgcg
    21661 cctttgtttg taatgaatgc ggggccgatt atccgcgctg gcaggggcag tgcagcgcct
    21721 gccatgcctg gaacaccatc accgaggtgc gtcttgctgc gtcgccaacg gtggcgcgta
    21781 acgagcgtct cagtggctat gccggtagcg ccggggtggc aaaagtccag aaactttccg
    21841 atatcagcct tgaagagctg ccgcgttttt ccaccggatt caaagagttc gaccgcgtat
    21901 taggtggcgg ggtggtgccg ggaagcgcca ttctgattgg cggtaaccca ggagccggga
    21961 aatccacgct gctactgcaa acgctgtgca aactggccca gcagatgaaa acgctgtatg
    22021 tcaccggcga agagtcgctg caacaggtgg caatgcgcgc tcatcgcctc ggcctgccga
    22081 ctgacaatct caatatgttg tcggaaacca gcatcgaaca gatctgcctg attgccgaag
    22141 aagagcaacc gaagctgatg gtcattgact ccatccaggt gatgcatatg gcggatgtac
    22201 aatcatcacc tggcagcgtg gcgcaggtgc gtgaaacggc ggcttatctg acgcgcttcg
    22261 ccaaaacgcg cggtgtggcg attgtcatgg tggggcacgt aaccaaagat ggttcgctgg
    22321 caggccctaa agtgctggaa cactgtatcg actgctcggt gcttctggac ggcgatgccg
    22381 actcccgttt ccgcacctta cgcagccata aaaaccgttt cggcgcggtg aatgagctgg
    22441 gcgtcttcgc gatgactgaa cagggactgc gtgaagtcag taacccttcg gcaattttct
    22501 taagtcgcgg cgatgaagtg acctccggca gctcagtgat ggtggtgtgg gaaggaacgc
    22561 gtccgctgct ggtggagatc caggcgctgg tcgatcactc gatgatggcg aatccgcgcc
    22621 gcgtggcagt ggggctggag caaaaccgtt tggcgatcct gctggcggtg ttgcaccgtc
    22681 acggtgggtt gcaaatggcc gatcaggatg tatttgtgaa cgtggtcggc ggcgtgaagg
    22741 tgacggaaac cagtgccgat ttagcgttac tactggcgat ggtttccagc ctgcgcgaca
    22801 gaccgctacc gcaggatctg gtggtgtttg gtgaagtcgg gctggcaggg gagatccgcc
    22861 cggtgcccag cggccaggag cggatttctg aagcggcgaa acacggtttt cggcgggcga
    22921 ttgttccggc agccaacgtg ccgaaaaaag cgccggaagg gatgcagatt tttggcgtta
    22981 aaaaactctc cgacgcgctt agcgtgttcg acgacttata atgagagata cggagggagg
    23041 tatgtcgtca tttgattatc tgaaaactgc catcaagcaa cagggctgta cgctacagca
    23101 ggtggctgat gccagcggta tgaccaaagg gtatttaagc cagttactga atgccaaaat
    23161 taaaagcccc agcgcgcaaa agctggaggc gttgcaccgt tatttgggac ttgaatttcc
    23221 ccggcagaag aaaactattg gtgttgtatt cggtaagttt tacccgctgc ataccggaca
    23281 tatctacctt atccagcgtg cctgtagtca ggttgacgag ctgcatatca ttatgggctt
    23341 tgacgatacc cgtgaccgcg cactgttcga agacagcgcc atgtcgcagc agccaacggt
    23401 gccggatcgt ctgcgctggt tattgcaaac ttttaaatat cagaaaaata ttcgcattca
    23461 tgctttcaac gaagagggca tggagccgta tccgcatggc tgggatgtgt ggagcaacgg
    23521 catcaaaaag tttatggctg aaaaagggat ccagccggat ctgatctaca cctcggaaga
    23581 agccgacgcg ccgcagtata tggaacatct ggggatcgaa acggtgttgg tcgatccgaa
    23641 acgtactttt atgagtatca gtggtgcgca gatccgcgaa aatccgttcc gctactggga
    23701 atatattcct accgaagtga agccgttctt tgtgcgcacc gtggcgatcc tcggcggcga
    23761 atcgagcggt aaatccaccc tggtaaacaa acttgccaat atcttcaaca ctaccagtgc
    23821 atgggaatat ggtcgcgatt atgtcttttc gcatcttggc ggtgatgaga ttgcattgca
    23881 gtattccgac tacgataaaa tcgcgcttgg acacgctcaa tatattgatt ttgcagtaaa
    23941 atatgccaat aaagtggcgt ttatcgacac cgattttgtg acaactcagg cgttctgcaa
    24001 aaagtacgaa gggcgcgagc atccgttcgt acaggcgctg atcgatgaat accgttttga
    24061 tctggtgatt ctgctggaga acaacacgcc gtgggtggcg gatggtttac gcagtctcgg
    24121 cagttcggtg gatcgcaaag agttccagaa cttgctggtg gagatgctgg aagagaacaa
    24181 tatcgaattt gtgcgggttg aagaggacga ttacgacagc cgtttcctgc gctgtgtgga
    24241 gctggtacgg gagatgatgg gggagcaggg ataatcatct ttataagatg ttgagtgata
    24301 ttactgattc aaaagtgtac ttagtactcc attttggtgt acaatgtacc tgccagggag
    24361 gggtaatggg caaggcgata atatttatag agacgccaat gtttacgcgt cagatcaagc
    24421 agattgcaac cgatgatgaa ttaaaggaat tgcaaaaaga actcatcgga actcccgata
    24481 aaggtgatct cattcagcag actggtggat tgcgtaaagt ccgaatggct gcgggttcac
    24541 agggaaaaag tggcagtctc agaataattt actttctggc gacggaagag attatttatt
    24601 tgattatggc ctatccgaag aatgccaaag acagtctgac agatacggaa aaagctcaac
    24661 taaagaagtt gacgaaactg ctcaaaggtg aggtataaaa tgagtttttt tgatgagttg
    24721 aaaacctctc tggaagaggc tgtcgagatt aaacaaggtt taaaagaacc tgcacgggtg
    24781 acccgctacg aaattgcgga tgttaaagct attcgtgagc aactcaacgt ttcgcaaagt
    24841 gagatggcaa aagctctggg aaccagtgtg gataccatta aaagctggga atcgaagcga
    24901 agaaatccta cgggattggc tgcaaaagta cttgccacaa ttaaagaaaa tcctgccttt
    24961 ttccgggaac ttgcctccca ttaatcattt aactattacc ggatgcgatg ctggcgcgcc
    25021 gcatccggca ttttacgcat tacttcgcaa tacgcttgta cttgatacgc ttcggctcca
    25081 gcgcgtctgc gcccagcgtg cgtttcttgt actcttcgta ctcggtaaag ttaccttcaa
    25141 agaactcaac tttaccttca tcctggtaat ccagaatgtg tgtggcgata cggtcgagga
    25201 accaacggtc gtgcgagata accatcgcgc agcccgggaa ctccagcagg gcgttttcca
    25261 gcgcacgcag ggtttcgata tccaggtcgt tggtcggttc atcgagcagc agcatgttgc
    25321 cgccaacctg cagcagcttc gccagatgca gacgaccacg ctcaccaccg gagagttcac
    25381 caacgcgttt accctgatca acccctttaa agttaaagcg gccaacgtag gcgcggcttg
    25441 gcatctcggt gttaccgatc ttcatgatat ccagcccgcc ggaaacttct tcccaaacgg
    25501 ttttgctgtt atccattgag tcacggaact gatcgacaga tgccagtttc accgtttcac
    25561 ctaaagtgat ggtgccgctg tctggctgtt cctgaccaga gatcatacgg aacagggttg
    25621 atttacccgc gccgttcgga ccgatgatcc cgacgatcgc ccctttaggg atcgagaagc
    25681 tcagatcatc aatcagcaga cgatcgccgt aagatttacg caggttgctg acttccagca
    25741 ctttatcgcc cagacgcggt ccaggtggaa taaacagttc gttggtttcg ttacgtttct
    25801 gatattcggt gctgttcagc tcttcaaaac gcgccagacg tgctttacct ttcgactgac
    25861 ggcctttagt cccctgacgt acccactcca gctctttctc aatcgactta cgacgcgcag
    25921 cttcttgtga agcttcctgc gccagacgtt gatctttctg ctccagccag gaggagtagt
    25981 taccttccca cggaatacct tcaccacggt ccagttcgag gatccagccc gccacgttat
    26041 cgaggaagta acggtcgtgg gtaatcgcca caacggtgcc ttcgaagtcg tgcaggaagc
    26101 gttccagcca cgccacggat tcggcatcca ggtggttcgt cggttcgtcg agcagcagca
    26161 tgtctggttt ttccagcagc aggcggcaca acgccacgcg acgacgctca ccaccggaga
    26221 ggttagcgat tttcgcgtcc cagtccggca gacgcagcgc atccgccgca cgctccagct
    26281 gaacgttcag gttgtgaccg tcgtgagcct gaatgatctc ttccagacgg ccttgttcag
    26341 cggccagctt gtcaaaatcg gcatccggat cggcgtacag cgcatacact tcatccaggc
    26401 gtttcagggc gttaaccact tcagaaaccg cttcttcaat ggactcacgc acggtgtgtt
    26461 ccgggttcag ctgcggttcc tgcggcagat aaccaatctt gatatccggt tgcggacgcg
    26521 cttcgccttc gatgtcttta tcaatgcccg ccataatgcg cagcagggtg gacttacccg
    26581 cgccgttcag acccaggaca ccaatttttg ccccagggaa gaaactcaga gagatgtttt
    26641 tcaaaatatg acgtttcggc ggaacaactt tgccgacacg atgcatggta taaacgaatt
    26701 gagccacgtt ggacttcgcc tctatgttta tcgtgatgat gagttttcaa aggcgaagtg
    26761 tagcctgttt ccttatctaa tcccagccgg tcgatcaccc tcccgataaa agtaaaaaag
    26821 tgtccgtaac gtggcgtaaa cgtcaatgac tggttagcat aaatctatta cgcggcatga
    26881 cgctgcattg atgtatttac acttagagga tgcgcttgtg gaaaaagcca aacacgttac
    26941 ctggcggctg ttggctgccg gtgtctgtct gctgacggtc agcagcgtgg cgcgagccga
    27001 ctcactggat gagcagcgta gccgctacgc ccaaatcaag caggcctggg ataatcgaca
    27061 aatggatgtg gtcgaacaaa tgatgcctgg actgaaagat tatccgcttt atccctacct
    27121 ggaataccgc cagatcaccg atgatctgat gaatcaaccg gcggtgacgg tcactaactt
    27181 tgttcgcgct aaccccacgc ttcctcccgc tcgcacgctg caatctcgtt tcgtcaatga
    27241 actggcgcgg cgtgaagact ggcgtggctt gttagccttt agcccggaaa agcccggaac
    27301 taccgaagcg caatgtaatt actactatgc aaaatggaac accgggcaga gtgaagaagc
    27361 ctggcaaggg gcgaaagagc tgtggctaac cggcaagagc cagcctaacg cctgtgacaa
    27421 gttatttagc gtctggcgtg cgtcaggtaa acaagatccg ctggcgtatt tagagcgtat
    27481 ccgtctggcg atgaaagcgg gtaacactgg cctggtaaca gtgctggcag ggcagatgcc
    27541 tgccgattac cagactatcg cttcggcaat tatttcactg gcgaacaatc ctaatacggt
    27601 actgaccttc gcgcgtacaa ccggagcgac cgattttacc cgtcaaatgg cggcggtggc
    27661 gtttgccagt gtggcgcggc aggatgctga aaatgcacgg ctgatgatcc cgtcgcttgc
    27721 ccaggcgcag cagcttaatg aagatcagat tcaggagctg cgcgatatcg tcgcctggcg
    27781 tttgatgggc aacgatgtca ccgacgaaca ggcgaaatgg cgtgatgacg ccattatgcg
    27841 ttcgcaatct acttcgctta ttgaacgccg tgtgcgaatg gcgcttggca ccggcgatcg
    27901 tcgcggcctg aatacctggc tggcgcgttt gccgatggag gcgaaagaga aagatgaatg
    27961 gcgttactgg caggcggatt tactgctgga acgcggacgt gaagctgaag caaaagagat
    28021 tttgcatcaa ctcatgcaac agcgtggttt ctacccgatg gttgcagcac aacgcatcgg
    28081 agaagagtat gagctgaaga ttgataaagc gccgcagaat gttgacagcg ccctgactca
    28141 ggggccggag atggcgcgcg tgcgcgagtt gatgtactgg aatctcgata acaccgcgcg
    28201 tagcgagtgg gccaatctgg tgaagagcaa gtcaaaaaca gagcaggcgc aactggctcg
    28261 gtatgctttc aataaccaat ggtgggatct tagcgttcag gcaacgatcg ccgggaagct
    28321 ttgggatcat ctggaagaac gattcccgct ggcctacaac gatcttttca aacgttacac
    28381 cagtgggaag gagatcccgc aaagctatgc gatggcgatt gcccgtcagg agagtgcctg
    28441 gaatccgaaa gtgaaatcgc cggtaggggc cagcggcctg atgcagatta tgccgggcac
    28501 ggcgacccat acggtgaaga tgttctctat tcctggttat agcagcccag gacaattgct
    28561 ggatccggaa acgaacatca acattggcac cagttacctg caatatgttt atcagcagtt
    28621 tggtaataac cgtattttct cctcagcagc ttataacgcc ggaccggggc gggtgcgaac
    28681 ctggcttggc aacagcgccg ggcgtatcga cgcagtggca tttgtcgaga gtattccgtt
    28741 ctccgagacg cgcggttatg tgaagaacgt gctggcttat gacgcttact accgctattt
    28801 catgggggat aaaccgacat tgatgagcgc cacggaatgg ggacgtcgtt actgatccgc
    28861 acgtttatga tatgctatcg tactctttag cgagtacgac cgggggaggc attttgcttc
    28921 ccccgctaac aatggcgaca tattatggcc caacaatcac cctattcagc agcgatggca
    28981 gaacagcgtc accaggagtg gttacgtttt gtcgacctgc ttaagaatgc ctaccaaaac
    29041 gatctccatt taccgttgtt aaacctgatg ctgacgccag atgagcgcga agcgttgggg
    29101 actcgcgtgc gtattgtcga agagctgttg cgcggcgaaa tgagccagcg tgagttaaaa
    29161 aatgaactcg gcgcgggcat cgcgacgatt acgcgtggat ctaacagcct gaaagccgcg
    29221 cccgtcgagc tgcgccagtg gctggaagag gtgttgctga aagatcactg atacaccgca
    29281 ttatgaaacg gactcagcgc caggatcacc gcctggtgat agacgctggc gcgggtaagt
    29341 ttcccggcgg taaacacgcc gatcgcccct tccttacgac caatctcatc aataccggta
    29401 taacgcgaca tcacgggacc aagcgcctca ccttcacgca ctttttccag aatcaccgca
    29461 ggcaacggca aagtagccga acgcgcctcg ccgcgctggc tggcgttttc aatcaccacc
    29521 caactgaagg tgctgtcgcc atcaatgcca gcttcaatcg ccacccaaaa atcagcctct
    29581 ggatgtaaac ggcgggcatt tgctacccga tttcgtgcgc cagcgcgcgt ttcctcactg
    29641 ccaaagggct gttccggtac accgctctcg acggcaacgg atgcaatatg gcaggatcct
    29701 tcgccgaaga tctcgtgaaa tgcctgcaga atggcctgaa ttttagcggg attggtggtc
    29761 gcacagacaa cttggtgcat aatcagcatt actcagaaaa ttaacgttac agcagtatac
    29821 ggaaaaaaag catgttacag gtatacctag tccgccacgg tgaaacgcag tggaacgccg
    29881 agcgacgtat tcagggccag tctgacagcc cgctgaccgc caaaggtgag caacaggcga
    29941 tgcaggtggc aacccgtgcc aaagagcttg gcattacgca tatcatcagt agcgatttag
    30001 gacgcacccg gcgtacggcg gaaatcatcg cccaggcctg cggctgtgac atcatctttg
    30061 attctcgcct gcgtgaatta aacatgggtg tgctggaaaa aagacatatc gattctctga
    30121 ccgaagaaga agagaactgg cgacggcagc tggtcaatgg caccgttgac gggcgtattc
    30181 ctgaaggcga gtcaatgcag gagctcagcg atcgcgtcaa tgcggcactg gaatcttgcc
    30241 gggacttacc gcagggaagc cgaccgctgc tggtgagcca cggtattgca ctgggatgcc
    30301 tggtgagtac gattctcgga ttaccagcat gggcagagcg ccgcttacgt ctgcgtaact
    30361 gttctatttc gcgcgtggat tatcaggaaa gcctgtggct ggcgtctggc tgggtcgttg
    30421 aaaccgcagg ggacatctcg catctggacg cccctgcatt agatgagctg cagcgttaac
    30481 gacggatcgg aatcagcagt tcacagcgca gattaattgg gcgatctccc gctttggcat
    30541 cttctgccgg gtagtatcgc tcaatatcct gacctttacg gcgcgtcagg ttgagcattg
    30601 gcatacacgt tccgtatacc gtcaggataa actcctgcac gccggttccc agaccttcat
    30661 aggtaaacat cacatattcg ccgccctgca gcatcaccgg atgccccgtc agtacatagc
    30721 catctgcctg atcctgggct aacgcggtgg tatagaatac ctcttgttcg tcgtctttat
    30781 cctgactcgg acgcgtttca ttcaggccgt agagcaccgg cggaatggtc ggcgcgttgc
    30841 cgagaaaatc gtgccagaac tgataacgca tttcatggcg gaaatcagag atttgctcca
    30901 gcgaacagga gtagctctgg gtaacaccaa tcagcggcgt atcttccagg gtgacaaatt
    30961 tgtgctctgg catagtgaat tcacccaggc gtagcggcgg gcgaatacca aaggcgctcc
    31021 attcaggaga acggcggtaa agtgcaggag tctgggcaaa ctgcttcttg aatgcgcggg
    31081 taaatgtctg ttgagagtcg aagcggtatt gcagcgcgat gtccagaatc ggacgcgcag
    31141 tcaggcgtag tgcgaccgcc gatttcgaca aacgacgagc acgaatatac gcgccaatag
    31201 catggccagt gacatcttta aacattctct gtaagtgcca cttggaataa cctgctttcg
    31261 ccgctacatt gtcgagcgac aggggctgat ccagatgacc ttccagccag attaaaaggt
    31321 cgcgaataat gccggcctga tccataaaat atcctcatcc tttcaacaac gagcacctga
    31381 catcaggtaa ttggataata gcattttttg ctgttttagc attcagtgtt tttttcttag
    31441 tagagtatgt tttaggactt gacggaaata aaagtattga gattttgttc ttaatcaata
    31501 tgttatttac cgtgacgaac taattgctcg tgtaatagat aaaaatggta acaatatgaa
    31561 atacaagcat ttgatcctgt ctttaagcct gataatgctg gggccattgg ctcatgcaga
    31621 agagattggt tcggtcgaca ccgtatttaa aatgatcggc ccggatcaca aaattgtcgt
    31681 ggaagccttt gacgatcccg atgtgaaaaa tgtcacctgt tatgtgagcc gggcgaaaac
    31741 cggtggtatt aaagggggat tgggcctggc agaagatacc tccgatgcgg ccatttcttg
    31801 tcagcaagtc gggccgattg aactgtcgga tcgtattaaa aacggcaaag ctcagggcga
    31861 ggtagtattc aaaaaacgca cgtccctggt ctttaagtcg ttacaggtcg tgcgctttta
    31921 tgacgccaaa cgcaacgcgc tcgcttatct ggcttactcc gacaaagttg tagaaggttc
    31981 gccgaaaaac gcgattagcg cggttcctgt catgccgtgg cggcaataac agaggcgatt
    32041 tatgcaacgg gaaacggtct ggttagtgga agatgagcaa gggatagccg acacgctggt
    32101 ctacatgttg cagcaggaag gttttgccgt cgaggtcttt gagcgaggct tgccggtgct
    32161 ggataaagct cgccagcagg ttcccgatgt catgattctc gacgttggtc tgccggatat
    32221 tagcggcttt gaattgtgcc gccagttact ggcgctccat ccggcgttac ctgtactgtt
    32281 cctgacggcc cgaagtgaag aggtcgatcg cctgcttggg ctggaaattg gcgctgacga
    32341 ctatgtggct aaaccgtttt caccccgcga agtgtgcgcc agggtgcgca ccttactgcg
    32401 tcgggtgaag aagttctcga cgccgtctcc cgtcatccat attggtcatt ttgaattgaa
    32461 tgaacccgcg gcgcagatca gctggtttga cacgccatta acgctgacgc ggtatgagtt
    32521 tttattgctg aagacgttac tcaagtcgcc aggccgcgtc tggtcccgcc agcaactgat
    32581 ggatagcgtg tgggaagatg cacaggacac ctacgatcgc accgtcgata cccacattaa
    32641 aacgttgcgt gccaagctgc gcgccatcaa ccccgatctt tcaccgatta atactcatcg
    32701 cggcatggga tatagcctga ggggcctgta atgcgtatcg gcatgcggtt gctgctgggc
    32761 tattttttac tggtggcggt ggcggcctgg ttcgtactgg ctatttttgt caaagaagtt
    32821 aaaccgggcg tgcgaagagc aacggagggg acgttgatcg acaccgcaac gttgctggcg
    32881 gagctggcgc gtcccgattt gctctctggg gacccaacgc atgggcaact ggcgcaggcg
    32941 tttaatcagc tacagcatcg cccgtttcgc gccaatatcg gtggcattaa caaagtgcgc
    33001 aatgaatatc atgtctatat gaccgatgcg cagggcaaag tattgttcga ttcggcaaat
    33061 aaagccgttg gacaggatta ttcgcgctgg aatgacgtct ggctaacgtt gcgtggtcag
    33121 tatggtgcgc gcagcacgtt gcaaaatcct gccgatcccg aaagttctgt gatgtatgtt
    33181 gccgcgccga ttatggacgg ctcgcggctt attggcgttt tgagcgtagg caaaccgaac
    33241 gcggcgatgg ctccggtcat taagcgcagc gagcggcgaa ttttatgggc cagcgccatt
    33301 ttgttgggga ttgcactggt gattggcgca ggcatggttt ggtggatcaa ccgctccatt
    33361 gcccggctca ctcgctatgc tgattccgtc actgacaata agcccgttcc tcttcccgat
    33421 ctcggtagta gcgagctgcg taaactcgcg caggcgctgg aaagtatgcg cgtgaagctg
    33481 gaagggaaaa actatattga gcagtatgtt tacgcattaa ctcatgagct aaaaagccca
    33541 ctggcggcga ttcgtggagc ggcggaaatt ttacgcgaag gtccgccgcc ggaagtggtg
    33601 gctcgtttta ctgacaacat tctgacgcaa aatgcgcgta tgcaggcatt ggtagaaacg
    33661 ttactacgcc aggcaagact ggagaatcgt caggaagtcg ttctgactgc tgttgatgtg
    33721 gcggcattat tccgccgcgt cagcgaagcg cgcaccgtgc agttggcaga aaaaaaaatc
    33781 actctgcatg ttacgcccac cgaggttaac gttgctgctg aaccggcgtt actggagcag
    33841 gcgctgggaa atttactgga taacgccatc gattttaccc ccgagagcgg ttgcatcacg
    33901 ctaagcgccg aagtggagca ggaacacgtc acgcttaagg tgctggatac cggtagtggt
    33961 attcctgact acgcgctttc acgtattttt gaacgctttt actctttgcc tcgtgcaaat
    34021 gggcaaaaaa gcagcggtct ggggttggcg ttcgtcagtg aggtcgcccg tttgtttaac
    34081 ggcgaagtca cgctgcgcaa cgtgcaggaa ggtggcgtgc tggcttcgct tcgacttcac
    34141 cgtcacttca catagcttca aattcttccc acatagcctt cgtatcctgc tgccattgca
    34201 aaggagaaga ctatgttgaa atcccccctg ttctggaaaa tgaccaccct gtttggtgca
    34261 gtattgctgt tgttgattcc gataatgctg attcggcagg tgattgtcga acgtgctgat
    34321 taccgtagcg atgtggaaga tgcgattcgc caaagtacca gcgggccgca aaaactcgtt
    34381 gggccgctca tcgctattcc tgtgaccgag ctttatacgg tgcaggaaga ggataaaacc
    34441 gtggagcgga aacgaagttt tatccatttt tggttacctg agtcattgat ggttgatggc
    34501 aatcagaacg tggaagaacg caagataggg atttatacca gtcaggtctg gcacagtgat
    34561 ttaacgctaa aagccgattt cgatgtttcg cgtcttagcg aactcaacgc gccaaatatc
    34621 accttaggca agccatttat tgtgattagc gtcggggatg cgcgtggtat tggtgtggtg
    34681 aaagcgcctg aagttaacgg aacggcgctg accattgaac ccggcaccgg gttagagcaa
    34741 ggcgggcagg gcgtgcatat ccctttacct gaaggggact ggcggaagca gaacctgaag
    34801 ctgaatatgg ccctgaattt aagcggtacc ggcgatcttt ctgtggtgcc tggcgggcgt
    34861 aatagcgaaa tgaccttaac cagcaactgg ccgcatccca gttttttagg tgattttcta
    34921 ccagccaaac gggaagttag cgagtcaggt tttcaggcgc aatggcaaag cagctggttt
    34981 gctaataatc tcggtgagcg ttttgcttca ggcaatgata ccggctggga aaacttcccc
    35041 gcgtttagcg tcgcagtgac gacgccagcc gatcaatacc aattaacgga ccgggcgact
    35101 aagtacgcca ttctgctgat tgcactgact tttatggcgt tctttgtttt tgaaacgctc
    35161 accgcgcaac gtttacaccc aatgcaatat ttgctggtgg ggctttcatt ggtgatgttt
    35221 tatttgctct tgctggcgct ttctgaacat atcggtttta ccgtggcatg gataatcgcc
    35281 agtctgattg gggcgttaat gaacggtatt tatttgcagg cggtattgaa aggctggtgc
    35341 aacagcatgt tgtttaccct cgcgctgttg ttgctggatg gtgtgatgtg gggactactc
    35401 aactctgccg atagcgcgct gttgttggga accagtgtgc tggtggtggc gctggccggc
    35461 atgatgtttg tgacccgtaa tatcgactgg tatgcgtttt cactgccgaa aatgaaagcc
    35521 agtaaagaag ttacatcgga cgatgaatta cgtatctgga aataaggttg aaaaataaaa
    35581 acggcgctaa aaagcgccgt tttttttgac ggtggtaaag ccgattaatc ttccagatca
    35641 ccgcagaagc gataaccttc accgtgaatg gtggcgatga tttccggcgt atccggcgta
    35701 gattcgaaat gtttacgaat acggcggatc gtcacgtcta cagtacggtc gtgcggtttc
    35761 agctcacggc cggtcatttt cttcagcagt tcagcacggg actgaatttt gcctgggttt
    35821 tcacagaagt gaagcatggc gcggaactcg ctgcgcggca gcttgtactg ctcgccatca
    35881 gggccgatca acgaacggct gttgatgtcc agttcccaac cattgaactt gtagctttca
    35941 acgctacgac gttcttcgct gacagtaccc agattcatgg tacgggacag caggttgcgt
    36001 gcacgaatag tcagttcacg tgggttgaac ggtttggtga tgtagtcatc tgcaccgatt
    36061 tcgaggccga gaattttatc gacttcgttg tcacggccag tcaggaacat caacgcaaca
    36121 ttcgcctgct cgcgcagttc acgcgctaac agaagaccgt tcttacccgg cagattgata
    36181 tccatgatca ccaggttgat gtcatattca gagaggatct gatgcatttc cgcgccatct
    36241 gtcgcttcga aaacatcata gccttccgct tcgaaaatac ttttcaacgt gttgcgtgtt
    36301 accaactcgt cttcaacgat aagaatgtgc ggggtctgca tgtttgctac ctaaattgcc
    36361 aactaaatcg aaacaggaag tacaaaagtc cctgacctgc ctgatgcatg ctgcaaatta
    36421 acatgatcgg cgtaacatga ctaaagtacg taattgcgtt cttgatgcac tttccatcaa
    36481 cgtcaacaac atcattagct tggtcgtggg tactttccct caggacccga cagtgtcaaa
    36541 aacggctgtc atcctaacca ttttaacagc aacataacag gctaagaggg gccggacacc
    36601 caataaaact acgcttcgtt gacatatatc aagttcaatt gtagcacgtt aacagtttga
    36661 tgaaatcatc gtatctaaat gctagctttc gtcacattat tttaataatc caactagtt
//
LOCUS       DAHHTG010000036        35762 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.036, whole genome shotgun sequence.
ACCESSION   DAHHTG010000036 DAHHTG010000000
VERSION     DAHHTG010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 35762)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35762)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35762
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.036"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            365..1189
                     /gene="gadX"
                     /locus_tag="NBI74_001777"
     CDS             365..1189
                     /gene="gadX"
                     /locus_tag="NBI74_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadX"
                     /protein_id="HCD1932134.1"
                     /translation="MQPLHGNCLIAYARHKYILTMVNGEYRYFNGGDLVFADASQIQV
                     DKCVENFVLVSRDTLSLFLPMLKEEALKLHVHKKVPSLLVHHCTRDIPVFQEVAQLSQ
                     NKNLRYAEMLRKRALIFALLSVFLEDTQFIPLLLNVLQPNMRTRVCTVINNNIAHEWT
                     LARIASELLMSPSLLKKKLREEGTSYSQLLTECRMQRALQLIVIYGVSIKRIAVSCGY
                     HSVSYFIYVFRNYYGMTPTEYQERSAQELPNCGSAASMAAQGIFYGTDRSAEGIRL"
     gene            1558..2286
                     /gene="gadW"
                     /locus_tag="NBI74_001778"
     CDS             1558..2286
                     /gene="gadW"
                     /locus_tag="NBI74_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadW"
                     /protein_id="HCD1932135.1"
                     /translation="MAHVCSVILVRRSFDIHHEQQKISLHNESILLLDKNLADDFAFC
                     SLDTRRLDIEELTVCHYLQNIRQLPRNLGLHSKDRLLINQSPPIQLVTAIFDSFNDPR
                     VNSPILSKMLYLSCLSMFSHKKELIPLLFNSISTVSGKVERLISFDIAKRWYLRDIAE
                     RMYTSESLIKKKLQDENTCFSKILLASRMSMARRLLELRQIPLHTIAEKCGYSSTSYF
                     INTFRQYYGVTPHQFSQHSPGTFS"
     gene            complement(2649..5762)
                     /locus_tag="NBI74_001779"
     CDS             complement(2649..5762)
                     /locus_tag="NBI74_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit"
                     /protein_id="HCD1932136.1"
                     /translation="MANYFIDRPVFAWVLAIIMMLAGGLAIMNLPVAQYPQIAPPTIT
                     ISATYPGADAQTVEDSVTQVIEQNMNGLDGLMYMSSTSDAAGNASITLTFETGTSPDI
                     AQVQVQNKLQLAMPSLPEAVQQQGISVDKSSSNILMVAAFISDNGSLNQYDIADYVAS
                     NIKDPLSRTAGVGSVQLFGSEYAMRIWLDPQKLNKYNLVPSDVISQIKVQNNQISGGQ
                     LGGMPQAADQQLNASIIVQTRLQTPEEFGKILLKVQQDGSQVLLRDVARVELGAEDYS
                     TVARYNGKPAAGIAIKLATGANALDTSRAVKEELNRLSAYFPASLKTVYPYDTTPFIK
                     ISIQEVFKTLVEAIILVFLVMYLFLQNFRATIIPTIAVPVVILGTFAILSAVGFTINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVIAEDKLPPKEATHKSMGQIQRALVGIAVVLS
                     AVFMPMAFMSGATGEIYRQFSITLISSMLLSVFVAMSLTPALCATILKAAPEGGHKPN
                     ALFARFNTLFEKSTQHYTDSTRSLLRCTGRYMVVYLLIFAGMAVLFLRTPTSFLPEED
                     QGVFMTTAQLPSGATMVNTTKVLQQVTDYYLTKEKNNVQSVFTVGGFGFSGQGQNNGL
                     AFISLKPWSERVGEENSVTAIIQRAMIALSSINKAVVFPFNLPAVAELGTASGFDMEL
                     LDNGNLGHEKLTQARNELLSLAAQSPNQVIGVRPNGLEDTPMFKVNVNAAKAEAMGVA
                     LSDINQTISTAFGSSYVNDFLNQGRVKKVYVQAGTPFRMLPDNINQWYVRNASGTMAP
                     LSAYSSTEWTYGSPRLERYNGIPSMEILGEAAAGKSTGDAMKFMADLVAKLPAGVGYS
                     WTGLSYQEALSSNQAPALYAISLVVVFLALAALYESWSIPFSVMLVVPLGVVGALLAT
                     DLRGLSNDVYFQVGLLTTIGLSAKNAILIVEFAVEMMQKEGKTPVEAIIEAARMRLRP
                     ILMTSLAFILGVLPLVISHGAGSGAQNAVGTGVMGGMFAATVLAIYFVPVFFVVVEHL
                     FARFKKA"
     gene            complement(5787..6944)
                     /gene="mdtE"
                     /locus_tag="NBI74_001780"
     CDS             complement(5787..6944)
                     /gene="mdtE"
                     /locus_tag="NBI74_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter subunit MdtE"
                     /protein_id="HCD1932137.1"
                     /translation="MNRRRKLLIPLLFCGAMLTACDDKSAENAAAMTPEVGVVTLSPG
                     SVNVLSELPGRTVPYEVAEIRPQVGGIIIKRNFIEGDKVNQGDSLYQIDPAPLQAELN
                     SAKGSLAKALSTASNARITFNRQASLLKTNYVSRQDYDTARTQLNEAEANVTVAKAAV
                     EQATINLQYANVTSPITGVSGKSSVTVGALVTANQADSLVTVQRLDPIYVDLTQSVQD
                     FLRMKEEVASGQIKQVQGSTPVQLNLENGKRYGQTGTLKFSDPTVDETTGSVTLRAIF
                     PNPNGDLLPGMYVTALVDEGSRQNVLLVPQEGVTHNAQGKATALILDKDDVVQLREIE
                     ASKAIGDQWVVTSGLQAGDRVIVSGLQRIRPGIKARAISSSQENASTESKQ"
     gene            7004..7333
                     /locus_tag="NBI74_001781"
     CDS             7004..7333
                     /locus_tag="NBI74_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001205317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1932138.1"
                     /translation="MRDLQTSGIVGLSASKMGCKHSDRHGEVNDKKNVAVLPTVSASI
                     RATKGSTGGYTYQGNKVVFVSPSIITGGIEISICIPRHASHQFQLLMSLLKIRCDTQG
                     DDVAHTS"
     gene            complement(7283..7810)
                     /gene="gadE"
                     /locus_tag="NBI74_001782"
     CDS             complement(7283..7810)
                     /gene="gadE"
                     /locus_tag="NBI74_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadE"
                     /protein_id="HCD1932139.1"
                     /translation="MIFLMTKDSFLLQGFWQLKDNHEMIKINSLSEIKKVGNKPFKVI
                     IDTYHNHILDEEAIKFLEKLDAERIIVLAPYHISKLKAKAPIYFVSRKESIKNLLEIT
                     YGKHLPHKNSQLCFSHNQFKIMQLILKNKNESNITSTLNISQQTLKIQKFNIMYKLKL
                     RRMSDIVTLGITSYF"
     gene            complement(8609..9181)
                     /gene="hdeD"
                     /locus_tag="NBI74_001783"
     CDS             complement(8609..9181)
                     /gene="hdeD"
                     /locus_tag="NBI74_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-resistance protein HdeD"
                     /protein_id="HCD1932140.1"
                     /translation="MLYIDKATILKFDLEMLKKHRRAIQFIAVLLFIVGLLCISFPFV
                     SGDILSTVVGALLICSGIALIVGLFSNRSHNFWPVLSGFLVAVAYLLIGYFFIRAPEL
                     GIFAIAAFIAGLFCVAGVIRLMSWYRQRSMKGSWLQLVIGVLDIVIAWIFLGATPMVS
                     VTLVSTLVGIELIFSAASLFSFASLFVKKQ"
     gene            9436..9768
                     /gene="hdeA"
                     /locus_tag="NBI74_001784"
     CDS             9436..9768
                     /gene="hdeA"
                     /locus_tag="NBI74_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeA"
                     /protein_id="HCD1932141.1"
                     /translation="MKKVLGVILGGLLLLPVVSNAADAQKAADNKKPVNSWTCEDFLA
                     VDESFQPTAVGFAEALNNKDKPEDAVLDVQGIATVTPAIVQACTQDKKASFKDKVKGE
                     WDKIKKDM"
     gene            9884..10210
                     /gene="hdeB"
                     /locus_tag="NBI74_001785"
     CDS             9884..10210
                     /gene="hdeB"
                     /locus_tag="NBI74_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeB"
                     /protein_id="HCD1932142.1"
                     /translation="MNISSLRKAFIFMGAVAALSLVNAQSALAANESAKDMTCQEFID
                     LNPKAMTPVAWWMLHEETVYKGGDTVTLNETDLTQIPKVIEYCKKNPQKNLYTFKNQA
                     SNDLPN"
     gene            10274..10921
                     /locus_tag="NBI74_001786"
     CDS             10274..10921
                     /locus_tag="NBI74_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HCD1932143.1"
                     /translation="MTAEFIIRLILAVIACGAIGMERQMRGKGAGLRTHVLIGMGSAL
                     FMIVSKYGFADVLSLGHVGLDPSRIAAQVVTGVGFIGAGNILVRNQNIVGLTTAADIW
                     VTAAIGMVIGSGMYELGIYGSVMTLLVLEVFHQLTFRLMNKNYHLQLTLVNGNTVSML
                     DWFKQQKIKTDLVSLQENEDHEVVAIDIQLHATTSIEDLLRLLKGMAGVKGVSIS"
     gene            complement(10973..11743)
                     /locus_tag="NBI74_001787"
     CDS             complement(10973..11743)
                     /locus_tag="NBI74_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter ATP-binding protein"
                     /protein_id="HCD1932144.1"
                     /translation="MISAQNLVYSLQGRRLTDNVSLTFPGGEIVAILGPNGAGKSTLL
                     RQLTGYLQPDSGECRLFNKPLNEWSITELAKHRAVMRQNSHMAFPFSVQEVIQMGRHP
                     HRTGNQDNETAQIMALCDCQALANRDYRQLSGGEQQRVQLARLLVQLWEPTPSPKWLF
                     LDEPTSALDIHHQQHLFRLLRQLVHERQFNVCCVLHDLNLAARYADRVVLMQKGKVIA
                     NGKPQDVLTQQALTMLYGADITVLKDPANHSPLIVLDH"
     gene            complement(11740..12696)
                     /gene="chuU"
                     /locus_tag="NBI74_001788"
     CDS             complement(11740..12696)
                     /gene="chuU"
                     /locus_tag="NBI74_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter permease ChuU"
                     /protein_id="HCD1932145.1"
                     /translation="MGLSLLLLALVLFGASQGALKISFDALFDEEYRDIWLNIRLPRV
                     LLAVLVGAALATAGVIMQGLFRNPMADPGLLGVSSGSALMVGVAIVLPFSFPVVLVLY
                     EQMVFAIAGSLVVCTIIFLITQRHRDGSMMQLLLAGIAINALCGAAIGILSYIGDEQQ
                     LRQLTLWMMGNLGQAQWPTLLVASSFILPAIIATTCLAGTLNLLQLGDEEAHYLGVNV
                     KRKRQQLLLVSSLLVGAAVSVSGIIGFIGLVIPHLIRMTTGANHRWLIPCSALAGACL
                     LLMADTLARTLVQPAEMPVGLLTSLLGGPYFMWLILRNRRIT"
     gene            complement(12781..13404)
                     /gene="chuY"
                     /locus_tag="NBI74_001789"
     CDS             complement(12781..13404)
                     /gene="chuY"
                     /locus_tag="NBI74_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobilin reductase"
                     /protein_id="HCD1932146.1"
                     /translation="MTPWLLFGAGGKGVGARTLELALAEQRPVVAVIRHADAATKLAQ
                     QGVQVFTGDACDASVVAAACRAAGPDALIISTMGGAQDYLAHRTVIDEAEKAGISRMI
                     LVTSLGCGDSWPFLSERAKAAFGQAVREKTLAESWLQTSQLDYAILRPGGLLDGAATG
                     KAQRIQNQECHGFVNRADVAAHIHELANAPALNQQVYSLIEPDLKPA"
     gene            complement(13404..13898)
                     /gene="hutX"
                     /locus_tag="NBI74_001790"
     CDS             complement(13404..13898)
                     /gene="hutX"
                     /locus_tag="NBI74_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme utilization cystosolic carrier protein
                     HutX"
                     /protein_id="HCD1932147.1"
                     /translation="MSHVSLQEFLKTEPDGTLEAIAEQYNTTLLEVVKNLPSPTVVSG
                     DKFDTVWDTVCEWGNVTTLVHTTDVILEFSGELPSGFHRHGYFNLRGKHGMSGHIKAE
                     NCTHIALIERKFMGMDTASILFFNKEGSAMLKIFLGRDDHRQLLSEQVSAFHALAASL
                     KEHA"
     gene            complement(13911..15248)
                     /locus_tag="NBI74_001791"
     CDS             complement(13911..15248)
                     /locus_tag="NBI74_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme anaerobic degradation radical SAM
                     methyltransferase ChuW/HutW"
                     /protein_id="HCD1932148.1"
                     /translation="MNTNNTLDLTPHFALDGDQPFKDRRAMMPFRGAIPVAKEQLAQT
                     WQEMINQTVSPRKRLVYLHIPFCATHCTFCGFYQNRFNEDACAHYTDALIREIELEAD
                     SVLHQSGPIHAVYFGGGTPSALSAHDLARIITTLREKLPLAPDCEITIEGRVLNFDAE
                     RIDACLDAGANRFSIGIQSFNSKIRKKMARTSDGPTAIAFMESLVKRDRAAVVCDLLF
                     GLPGQDAQTWGEDLAIARDIGLDGVDLYALNVLPNTPLGKAVENGRTTVPSPAERRDL
                     YLQGCDFMDDAGWRCISNSHWGRTTRERNLYNLLIKQGADCLAFGSGAGGSINGYSWM
                     NERNLQTWHESVAAGKKPLMMIMRNAERNAQWRHTLQSGIETARVPLDELTPHAEKLA
                     PLLAQWHQKGLSRDASTCLRLTNEGRFWASNILQSLNELIQVLNAPAIALEKP"
     gene            complement(15268..16182)
                     /gene="chuT"
                     /locus_tag="NBI74_001792"
     CDS             complement(15268..16182)
                     /gene="chuT"
                     /locus_tag="NBI74_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312409.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter substrate-binding protein
                     ChuT"
                     /protein_id="HCD1932149.1"
                     /translation="MPRIITRPFLFSPLTLCISAVVSAAKTMVKRKKLFTALLALSWT
                     FSVTAAERIVVAGGSLTELIYAMGAGKRVVGVDETTSYPPETAKLPHIGYWKQLSSEG
                     ILSLRPDSVITWQDAGPQIVLDQLRAQKVNVVTLPRVPATLEQMYANIRQLAKTLQVP
                     EQGEALVTQINQRLERVQQNVAAKKAPVKAMFILSAGGSAPQVAGKGSVADAILSLAG
                     AENVATHQQYKSYSAESLIAANPEVIVVTSQMVDGDINRLRSIAGITHTAAWKNQRII
                     TVDQNLILGMGPRIADVVESLHQQLWPQ"
     gene            16867..18849
                     /gene="chuA"
                     /locus_tag="NBI74_001793"
                     /gene_synonym="shuA"
     CDS             16867..18849
                     /gene="chuA"
                     /locus_tag="NBI74_001793"
                     /gene_synonym="shuA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent heme/hemoglobin receptor
                     ChuA/ShuA"
                     /protein_id="HCD1932150.1"
                     /translation="MSRPQFTSLRLSLLALAVSATLPTFAFATETMTVTATGNARSSF
                     EAPMMVSVIDTSAPENQTATSATDLLRHVPGITLDGTGRTNGQDVNMRGYDHRGVLVL
                     VDGVRQGTDTGHLNGTFLDPALIKRVEIVRGPSALLYGSGALGGVISYDTVDAKDLLQ
                     EGQSSGFRVFGTGGTGDHSLGLGASAFGRTENLDGIVAWSSRDRGDLRQSNGETAPND
                     ESINNMLAKGTWQIDSAQSLSGLVRYYNNDAREPKNPQTVEASDSSNPMVDRSTIQRD
                     AQLSYKLAPQGNDWLNADAKIYWSEVRINAQNTGSSGEYREQITKGARLENRSTLFAD
                     SFASHLLTYGGEYYRQEQHPGGATTGFPQAKIDFSSGWLQDEITLRDLPITLLGGTRY
                     DSYRGSSDGYKDVDADKWSSRAGMTINPTNWLMLFGSYAQAFRAPTMGEMYNDSKHFS
                     IGRFYTNYWVPNPNLRPETNETQEYGFGLRFDDLMLSNDALEFKASYFDTKAKDYIST
                     TVDFAAATTMSYNVPNAKIWGWDVMTKYTTDLFSLDVAYNRTRGKDTDTGEYISSINP
                     DTVTSTLNIPIAHSGFSVGWVGTFADRSTHISSSYSKQPGYGVNDFYVSYQGQQALKG
                     MTTTLVLGNAFDKEYWSPQGIPQDGRNGKIFVSYQW"
     gene            18898..19926
                     /gene="chuS"
                     /locus_tag="NBI74_001794"
     CDS             18898..19926
                     /gene="chuS"
                     /locus_tag="NBI74_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hematinate-forming heme oxygenase ChuS"
                     /protein_id="HCD1932151.1"
                     /translation="MNHYTRWLELKEQNPGKYARDIAGLMNISEAELAFARVTHDAWR
                     MRGDIREILAALESVGETKCICRNEYAVHEQVGAFTNQHLNGHAGLILNPRALDLRLF
                     LNQWASVFHIKENTARGERQSIQFFDHQGDALLKVYATDNTDMAAWSELLARFITDEN
                     TPLELKAVDAPVVQTRADASVVEQEWRAMTDVHQFFTLLKRHNLTRQQAFNLVADDLA
                     CKVSNSALAQILESAQQDGNEIMVFVGNRGCVQIFTGVVEKVVPMKGWLNIFNPTFTL
                     HLLEESIAEAWVTRKPTSDGHVTSLELFAHDGTQIAQLYGQRTEGEQEQAQWRKQIAS
                     LIPEGVTA"
     gene            complement(19983..20513)
                     /locus_tag="NBI74_001795"
     CDS             complement(19983..20513)
                     /locus_tag="NBI74_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCD1932152.1"
                     /translation="MFLIITRDTMFFTAMKNILSKGNVVHIQNEEEIDVMLHQNAFVI
                     IDTLMNNVFHSNFLTQIERLKPVHVIIFSPFNIKRCLGKVPVTFVPRTIAIIDFVALI
                     NGSYCSVPEADVSLSRKQHQVLSCIANQMTTEDILEKLKISLKTFYCHKHNIMMILNL
                     KRINELVRHQHINYLV"
     gene            complement(20666..21232)
                     /gene="slp"
                     /locus_tag="NBI74_001796"
     CDS             complement(20666..21232)
                     /gene="slp"
                     /locus_tag="NBI74_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312404.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein Slp"
                     /protein_id="HCD1932153.1"
                     /translation="MNMTKGALILSLSFLLAACSSIPQNIKGNNQPDIQKSFVAVHNQ
                     PGLYVGQQARFGGKVINVINGKTDTLLEIAVLPLDSYAKPDIEANYQGRLLARQSGFL
                     DPVNYRNHFVTILGTIQGEQPGFINKVPYNFLEVNMQGIQVWHLREVVNTTYNLWDYG
                     YGAFWPEPGWGAPYYTNAVSQVTPELVK"
     gene            complement(21422..21801)
                     /locus_tag="NBI74_001797"
                     /pseudo
     CDS             complement(21422..21801)
                     /locus_tag="NBI74_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709288.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(22418..22843)
                     /gene="arsC"
                     /locus_tag="NBI74_001798"
     CDS             complement(22418..22843)
                     /gene="arsC"
                     /locus_tag="NBI74_001798"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-dependent arsenate reductase"
                     /protein_id="HCD1932154.1"
                     /translation="MSNITIYHNPACGTSRNALEMIRNSGTEPTIIYYLETPPTRDEL
                     VKLIADMGITVRALLRKNVEPYEELGLAEDKFTHDQLIDFMLQHPILINRPIVVTPQG
                     TRLCRPSEVVLEILPDAQKGAFTKEDGEKVVDEAGNRLK"
     gene            23178..23261
                     /locus_tag="NBI74_001799"
     CDS             23178..23261
                     /locus_tag="NBI74_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Damage inducible protein"
                     /protein_id="HCD1932155.1"
                     /translation="MIDKAIIVLGALIAQLELIRFLLQLLN"
     gene            complement(23315..24667)
                     /gene="gorA"
                     /locus_tag="NBI74_001800"
     CDS             complement(23315..24667)
                     /gene="gorA"
                     /locus_tag="NBI74_001800"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HCD1932156.1"
                     /translation="MTKHYDYIAIGGGSGGIASINRAAMYGQKCALIEAKELGGTCVN
                     VGCVPKKVMWHAAQIREAIHMYGPDYGFDTTINKFNWETLIASRTAYIDRIHTSYENV
                     LGKNNVDVIKGFARFVDAKTIEVNGETITADHILIATGGRPSHPDIPGVEYGIDSDGF
                     FALPALPERVAVVGAGYIAVELAGVINGLGAKTHLFVRKHAPLRSFDPMISETLVEVM
                     NAEGPQLHTNAIPKAVVKNADGSLTLELEDGRSETVDCLIWAIGREPANDNINLEAAG
                     VKTNEKGYIVVDKYQNTNVEGIYAVGDNTGAVELTPVAVAAGRRLSERLFNNKPDEHL
                     DYSNIPTVVFSHPPIGTVGLTEPQAREQYGDDQVKVYKSSFTAMYTAVTTHRQPCRMK
                     LVCIGPEEKIVGIHGIGFGMDEMLQGFAVALKMGATKKDFDNTVAIHPTAAEEFVTMR
                     "
     gene            complement(24739..25581)
                     /gene="rlmJ"
                     /locus_tag="NBI74_001801"
     CDS             complement(24739..25581)
                     /gene="rlmJ"
                     /locus_tag="NBI74_001801"
                     /EC_number="2.1.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase"
                     /protein_id="HCD1932157.1"
                     /translation="MLSYRHSFHAGNHADVLKHTVQSLIIESLKEKDKPFLYLDTHAG
                     AGRYQLGSEHAERTGEYLEGIARIWQQDDLPAELEAYINVVKHFNRSGQLRYYPGSPL
                     IARQLLREQDSLQLTELHPSDYPLLRSEFQKDSRARVEKADGFQQLKAKLPPVSRRGL
                     ILIDPPYEMKTDYQAVVSGIAEGYKRFATGTYALWYPVVLRQQIKRMIHDLEATGIRK
                     ILQIELAVLPDSDRRGMTASGMIVINPPWKLEQQMNNVLPWLHSKLVPAGTGHATVSW
                     IVPE"
     gene            25784..27826
                     /gene="prlC"
                     /locus_tag="NBI74_001802"
     CDS             25784..27826
                     /gene="prlC"
                     /locus_tag="NBI74_001802"
                     /EC_number="3.4.24.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020317218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase A"
                     /protein_id="HCD1932158.1"
                     /translation="MTNPLLTPFELPPFSKILPEHVVPAVTKALNDCRENVERVVAQG
                     APYTWENLCQPLAEVDDVLGRIFSPVSHLNSVKNSPELREAYEQTLPLLSEYSTWVGQ
                     HEGLYKAYRDLRDGDHYATLNTAQKKAVDNALRDFELSGIGLPKEKQQRYGEIATRLS
                     ELGNQYSNNVLDATMGWTKLVTDEAELAGMPESALAAAKAQAEAKELEGYLLTLDIPS
                     YLPVMTYCDNQALREEMYRAYSTRASDQGPNAGKWDNSKVMEEILALRHELAQLLGFE
                     NYAFKSLATKMAENPQQVLDFLTDLAKRARPQGEKELAQLRAFAKAEFGVDELQPWDI
                     AYYSEKQKQHLYSISDEQLRPYFPENKAVNGLFEVVKRIYGITAKERKDVDVWHPDVR
                     FFELYDENNELRGSFYLDLYARENKRGGAWMDDCVGQMRKADGSLQKPVAYLTCNFNR
                     PVNGKPALFTHDEVITLFHEFGHGLHHMLTRIETAGVSGISGVPWDAVELPSQFMENW
                     CWEPEALAFISGHYETGEPLPKELLDKMLAAKNYQAALFILRQLEFGLFDFRLHAEFR
                     PDQGAKILETLAEIKKLVAVVPSPSWGRFPHAFSHIFAGGYAAGYYSYLWADVLAADA
                     FSRFEEEGIFNRETGQSFLDNILSRGGSEEPMELFKRFRGREPQLDAMLEHYGIKG"
     gene            27834..28586
                     /gene="rsmJ"
                     /locus_tag="NBI74_001803"
     CDS             27834..28586
                     /gene="rsmJ"
                     /locus_tag="NBI74_001803"
                     /EC_number="2.1.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709277.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1516)-N(2))-methyltransferase
                     RsmJ"
                     /protein_id="HCD1932159.1"
                     /translation="MKICLIDETGAGDGALSVLAARWGLEHDEDNLMALVLTPEHLEL
                     RKRDEPKLGGIFVDFVGGAMAHRRKFGGGRGEAVAKAVGIKGDYLPDVVDATAGLGRD
                     AFVLASVGCRVRMLERNPVVAALLDDGLARGYADAEIGGWLQERLQLIHASSLTALSD
                     ITPRPQVVYLDPMFPHKQKSALVKKEMRVFQSLVGPDLDADGLLEPARLLATKRVVVK
                     RPDYAPPLANVATPNAVVTKGHRFDIYAGTPV"
     gene            complement(28635..30104)
                     /gene="dtpB"
                     /locus_tag="NBI74_001804"
     CDS             complement(28635..30104)
                     /gene="dtpB"
                     /locus_tag="NBI74_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpB"
                     /protein_id="HCD1932160.1"
                     /translation="MNTTTPMGMLQQPRPFFMIFFVELWERFGYYGVQGVLAVFFVKQ
                     LGFSQEQAFVTFGAFAALVYGLISIGGYVGDHLLGTKRTIVLGALVLAIGYFMTGMSL
                     LKPDLIFIALGTIAVGNGLFKANPASLLSKCYPPKDPRLDGAFTLFYMSINIGSLIAL
                     SLAPVIADKFGYSVTYNLCGAGLIIALLVYIACRGMVKDIGSEPDFRPMSFSKLLYVL
                     LGSVVMIFVCAWLMHNVEVANLVLIVLSIVVTIIFFRQAFKLDKTGRNKMFVAFVLML
                     EAVVFYILYAQMPTSLNFFAINNVHHEILGFSINPVSFQALNPFWVVLASPILAGIYT
                     HLGNKGKDLSMPMKFTLGMFMCSLGFLTAAAAGMWFADAQGLTSPWFIVLVYLFQSLG
                     ELFISALGLAMIAALVPQHLMGFILGMWFLTQAAAFLLGGYVATFTAVPDNITDPLET
                     LPVYTNVFGKIGLVTLGVAVVMLLMVPWLKRMIATPESH"
     gene            complement(30421..30855)
                     /gene="uspA"
                     /locus_tag="NBI74_001805"
     CDS             complement(30421..30855)
                     /gene="uspA"
                     /locus_tag="NBI74_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspA"
                     /protein_id="HCD1932161.1"
                     /translation="MAYKHILIAVDLSPESKVLVEKAVSMARPYNAKVSLIHVDVNYS
                     DLYTGLIDVNLGDMQKRISEETHHALTELSTNAGYPITETLSGSGDLGQVLVDAIKKY
                     DMDLVVCGHHQDFWSKLMSSARQLINTVHVDMLIVPLRDEEE"
     gene            31246..31581
                     /gene="uspB"
                     /locus_tag="NBI74_001806"
     CDS             31246..31581
                     /gene="uspB"
                     /locus_tag="NBI74_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspB"
                     /protein_id="HCD1932162.1"
                     /translation="MISTVALFWALCVVCIVNMARYFSSLRALLVVLRNCDPLLYQYV
                     DGGGFFTSHGQPNKQMRLVWYIYAQRYRDHHDDEFIRRCERVRRQFILTSALCGLVVV
                     SLIALMIWH"
     gene            complement(31652..33151)
                     /gene="pitA"
                     /locus_tag="NBI74_001807"
     CDS             complement(31652..33151)
                     /gene="pitA"
                     /locus_tag="NBI74_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitA"
                     /protein_id="HCD1932163.1"
                     /translation="MLHLFAGLDLHTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMRSQLAVVMAAVFNFLGVLLGGLSVAYAIVHMLPTDLLLNMGSSHGLAMVFSMLL
                     AAIIWNLGTWYFGLPASSSHTLIGAIIGIGLTNALMTGTSVVDALNIPKVLSIFGSLI
                     VSPIVGLVFAGGLIFLLRRYWSGTKKRARIHLTPAEREKKDGKKKPPFWTRIALILSA
                     IGVAFSHGANDGQKGIGLVMLVLIGVAPAGFVVNMNATGYEITRTRDAINNVEAYFEQ
                     HPALLKQATGADQLVPAPEAGATQPAEFHCHPSNTINALNRLKGMLTTDVESYDKLSL
                     DQRSQMRRIMLCVSDTIDKVVKMPGVSADDQRLLKKLKSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVATTIGEKIGKKGMTYAQGMSAQMTAAVSIGLASYTGMPVSTTHVL
                     SSSVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAVLLSGGLYWLSLQFL"
     gene            33383..34576
                     /locus_tag="NBI74_001808"
     CDS             33383..34576
                     /locus_tag="NBI74_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001683372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HCD1932164.1"
                     /translation="MERFDAIIIGAGAAGMFCSALAGQAGRRVLLIDNGKKPGRKILM
                     SGGGRCNFTNLYVEPGAYLSQNPHFCKSALARFTQWDFIDLVNKHGITWHEKTLGQLF
                     CDDSAQQIVDMLVDECEKGNVAFRLRSEVLSVAKDDTGFTLELNGMTVGCEKLVIATG
                     GLSMPGLGASPFGYKIAEQFGLNVLPTRAGLVPFTLHKPLLEELQVLAGVAVPSVITA
                     ENGTVFRENLLFTHRGLSGPAVLQISSYWQPGEFVSINLLPDVDLETFLNEQRNAHPN
                     QSLKNTLAVHLPKRLVERLQQLGQIPDVSLKQLNVRDQQTLISTLTDWRVQPNGTEGY
                     RTAEVTLGGVDTNELSSRTMEARKVPGLYFIGEVMDVTGWLGGYNFQWAWSSAWACAQ
                     DLVAE"
     gene            complement(34663..35715)
                     /locus_tag="NBI74_001809"
     CDS             complement(34663..35715)
                     /locus_tag="NBI74_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417948.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2776 family protein"
                     /protein_id="HCD1932165.1"
                     /translation="MNIYIGWLFKLIPLLMGIICIALGEFVLTGSGQSEYFVAGHVLI
                     SLSAICLALFTTAFIIISQLTHSMNKFYNRLFPVIGYAGSATTMIWGWSLLASNNVMA
                     DEFVAGHVIFGVGMIAACVSTVAASSGHFLLIPKNASGSKSDGTPLQAYSSTIGNCLI
                     AVPVLLTLFGFIWSVILLRSADITPHYVAGHVLMGLTAICACLIGLVATIVHQTRNTF
                     SVKEHWLWCYWVILLGSLTIIFGIYVLISSDASARLAPGIILICLGMICYSIFSKVWL
                     LALVWRRTCSLANRIPMIPVFTCLFCLFLAAFLAEIAQTDMAYFIPSRVLVGLGAVCF
                     TLFSIVSILEAGSAKK"
BASE COUNT         9360 a         8508 c         8827 g         9067 t
ORIGIN      
        1 tcacgttgta aaaaccgaat gcccaacctt taaaaaacag gtggggtatt cggctactta
       61 ttaattgcca taatatatta cctgtctatt catcgtgttg ataagatata tgttatatga
      121 atgttatgga gcatagcgat actgcttaat attacgatga tgaattattt gcttactatt
      181 aattcctctg cgttcttaag tccccttttt atataataaa tccagtcatc cagcccgagt
      241 atcatcgctc attatcatcc tgttttcccg cttcgtttaa atttatttat caatcaattt
      301 gacttaagag ggcggcgtgc tacattaata aacagtaata tgtttatgta atattaagtc
      361 aactatgcaa ccactacatg gtaattgtct aattgcgtac gcaagacata aatatattct
      421 caccatggtt aatggtgaat atcgctattt taatggcggt gatctggttt ttgcggatgc
      481 aagccaaatt caagtggata agtgtgttga gaatttcgta ttagtttcaa gagacacgct
      541 ttcattattt ctcccgatgc tcaaggagga ggcattaaaa cttcatgtac ataaaaaagt
      601 tccttcatta ctcgttcatc actgtaccag agatattcct gtttttcagg aagttgcgca
      661 actatcgcag aataagaatc ttcgctatgc agaaatgcta cgtaaaaggg cattaatctt
      721 tgcgttgtta tctgtttttc ttgaggatac gcagtttata ccgttacttc tgaacgtgtt
      781 gcaaccgaat atgcgaacac gagtttgtac ggtcatcaat aataatatcg cccatgagtg
      841 gacattagcc cgaattgcta gcgaattgtt gatgagtcca agcctgttaa agaaaaaatt
      901 gcgcgaagaa ggtacgtcat attcacagtt gcttactgag tgtaggatgc agcgtgcttt
      961 acaacttatt gttatatatg gggtttcaat taagcgaatt gcagtatcct gtggatatca
     1021 cagcgtgtcg tatttcattt acgtttttcg aaattattat gggatgacgc ccacagagta
     1081 tcaggagcga tcggcgcagg aattaccgaa ctgtggctcg gcggcaagta tggctgcgca
     1141 agggattttt tacggcactg accgttctgc ggaaggaata agattatagc gttttactca
     1201 gacataaaaa aaacccggca taggggaccg ggaagaggat agtctgccgt ctccagacta
     1261 ataaaccgtt ataacactcc ctgttggcac gggaaacttt gtgctctcag taagttaaat
     1321 ataactttta ctggaaataa gatcagccat ttttttataa acataagcta tacgctgtgt
     1381 gaaaatataa tcgtgttgca tttacttatt atcaattaac tgttatgcaa aactactttg
     1441 tggataaaat ttggtcctac caaatatggc agtttttgca cacagaaaca gtctggcgtc
     1501 atttcattag tatactgaca ttgaaataat cgcagtaatg aaatataagg gatagtcatg
     1561 gctcatgtct gctcggtgat cctcgttcgt cgttcattcg atattcatca tgaacagcaa
     1621 aaaatatcgt tgcataacga gagtatcctg ctgctggata aaaatttggc agacgatttt
     1681 gcgttttgtt cactggatac gcgacggctg gatatcgaag agctgacagt ttgccattac
     1741 ttacaaaata ttcgtcagtt gccacgcaat ttaggattgc atagcaaaga ccgtttgtta
     1801 attaaccagt caccccccat acagctggtg acggcgattt ttgatagttt caatgacccc
     1861 cgggtcaatt cgccgatact gagcaaaatg ctctaccttt cctgtttatc aatgttttct
     1921 cataagaaag aactgatccc cttacttttc aatagtatca gtactgtttc aggaaaagtt
     1981 gaacgcctta ttagctttga tatcgctaaa cgttggtatc tacgcgatat cgcagaaaga
     2041 atgtacacca gcgagagtct catcaaaaaa aagttgcagg atgaaaatac ctgtttcagt
     2101 aaaatattac tcgcctccag gatgtcgatg gccagacgat tactcgagtt acgtcaaata
     2161 cctctgcata ctattgcgga aaaatgtggc tatagcagta cgtcatactt tataaataca
     2221 tttagacaat attatggtgt aacgccacat cagttttcgc aacattcgcc gggtaccttt
     2281 tcctgacata tttcgcattt gaatattggt caggatctca cgtctgcttc atgtgaaact
     2341 ctttcctgat gatttctgcc gggctaccgg ctagttctct ttcgcagttt gccaggcggt
     2401 ataggtattt tcaggaggaa aagactcggc atgtttggga ttattaagct aacaattcat
     2461 acgattacgg gaatgtgggt cagtattgtg cttttcaaat tgatgacgaa tggctggagt
     2521 ggtttctact ttcagtgctg tgtactaagc ctggtattcc tgacggttag ctggttgttg
     2581 agtggtgaat ggttagcagg aaagagtaag gctgaaccct cacgttcaac cttactctca
     2641 tttacacgtt acgctttttt aaagcgggca aagagatgtt ccactacaac gaaaaagacc
     2701 ggaacgaagt aaattgccag cactgttgcg gcaaacatcc cgcccattac gccggtacct
     2761 accgcgtttt gcgcgccaga gccggcacca tgactgataa ccagcggcag cacgccgaga
     2821 ataaaggcca gagaagtcat caggattggg cgtaaacgca tacgcgctgc ttcgatgatt
     2881 gcctctaccg gcgttttccc ttctttctgc atcatctcaa cggcaaattc gacaatcagg
     2941 atggcgttct tggcggaaag cccgatggtg gtcagcaaac caacctggaa gtaaacgtca
     3001 ttacttaagc cgcgcagatc ggtggccagt aatgcgccaa cgacgcctaa cggaacaacc
     3061 aacatcaccg agaacggaat tgaccagctt tcataaagtg cggcgagggc gaggaacacc
     3121 acgaccagtg aaatggcata tagcgcggga gcctggtttg aggataacgc ttcctgatac
     3181 gacagcccgg tccatgagta gccgacgcct gccggaagtt ttgcgaccag gtctgccata
     3241 aatttcatgg cgtcaccggt acttttcccg gcagccgctt cacctaaaat ctccattgac
     3301 gggatgccgt tgtagcgttc cagtcgcggt gaaccatagg tccattcggt agacgaatag
     3361 gcagaaagcg gtgccatcgt gccagaggcg ttgcgtacat accattggtt gatgttatcc
     3421 ggcaacatac ggaacggcgt gcctgcctgg acatacactt ttttcacccg cccctggttg
     3481 aggaagtcgt tcacgtagct gctgccgaag gcggtggaaa ttgtctggtt gatatcagac
     3541 agtgccacac ccatagcctc ggcttttgca gcgttgacgt tcactttgaa catcggcgta
     3601 tcttccaggc cgttcgggcg taccccgatg acctgattcg gtgattgcgc tgccagtgat
     3661 aatagctcgt ttcgcgcctg ggtcagtttt tcgtgcccca gattgccgtt gtccagcagt
     3721 tccatatcaa aacctgacgc ggtacccagt tcagccaccg cgggtaagtt gaacgggaag
     3781 acgacggctt tattgatact gcttagcgca atcattgccc gctgaataat cgcggtaacc
     3841 gagttttcct cgccgacacg ttcagaccac ggcttgaggc tgataaacgc cagaccgttg
     3901 ttttgcccct gaccactgaa gccaaagcca ccaacggtaa acaccgactg aacattattt
     3961 ttctcttttg tcagataata atccgttacc tgttgtagca ctttcgtggt gttaaccatg
     4021 gttgcaccgg aaggtaactg cgcggtggtc ataaataccc cctgatcctc ttctggtaag
     4081 aaagaggtcg gcgtacgcag gaacagcacc gccatcccgg cgaaaatcag caggtagacc
     4141 accatgtagc gaccggtaca acgcaacagc gagcgggtgc tgtcggtata gtgctgagtt
     4201 gatttttcaa acagcgtgtt gaagcgtgcg aacagggcgt taggtttgtg accgccctcc
     4261 ggcgcggctt tcagaatggt ggcgcacagg gcaggggtca ggctcattgc tacaaatact
     4321 gaaagcagca tggaggagat cagcgtgatg gagaactggc ggtagatttc cccggtcgca
     4381 ccgctcataa aggccatcgg cataaacact gcggaaagaa caacggcaat cccgaccagc
     4441 gcacgttgga tctgccccat cgatttatgc gtcgcttcct tcggcggtag cttatcttcc
     4501 gcaatgacac gctcgacgtt ctccaccacc acgatggcgt catccaccag taaccctatc
     4561 gccagcacca tcccgaacat agttaacgtg ttgatggtga aaccgacagc cgacaagatc
     4621 gcaaacgtcc cgagaataac caccggtacg gcaatcgtcg ggatgattgt ggcgcggaaa
     4681 ttctgcaaaa acagatacat gaccaggaag acgaggatga tagcctcaac cagcgttttg
     4741 aaaacttcct gaatagaaat tttgataaac ggtgtggtgt cgtaaggata aaccgtcttc
     4801 aggcttgcag ggaaataagc cgataaacgg ttcagctcct ctttcaccgc tcgcgaggta
     4861 tccagggcgt ttgctccggt agccagtttg atggcgatcc cggcagcagg tttgccgtta
     4921 tagcgcgcca cggtggaata atcttccgcc ccaagttcga cgcgggcgac atcacgcagc
     4981 agtacttgcg aaccatcttg ctgaactttc aacaggattt tgccaaattc ttccggcgtt
     5041 tgcagacgtg tctgcacaat gatcgaggcg ttcagttgct ggtctgccgc ctgtggcatg
     5101 ccacccagtt gaccaccgga aatctggttg ttttgcacct taatctggga aataacatcg
     5161 gaaggtacca ggttatattt attgagtttt tgcgggtcca gccagatacg catggcgtac
     5221 tcggaaccaa agagctgtac gctaccaacg cccgcggtac ggcttagcgg gtctttgata
     5281 ttagacgcta catagtctgc gatatcgtac tggttgagac tgccgttatc agaaataaat
     5341 gccgctacca tcaggatatt actgctcgac ttatcgacgc taatcccctg ctgctgcacc
     5401 gcttcaggta atgaaggcat agcgagttgc agtttatttt gcacctgaac ctgtgcgata
     5461 tcaggtgatg tccccgtctc gaaggtcaga gtgatggagg cattacctgc cgcatcgctg
     5521 gttgaagaca tgtacatcag gccatcaagc ccattcatat tttgctcaat cacctgagtg
     5581 accgagtctt ctaccgtttg cgcatcggca cccggatagg tagcgctgat ggtaatggtc
     5641 ggtggcgcaa tctgcggata ctgcgcaacc ggtaagttca tgatcgccag accgcctgca
     5701 agcatcataa taatggcaag tacccaggca aaaaccgggc gatcaataaa atagttagcc
     5761 atgaaagtcc ccttaagcct gcaacgttat tgtttcgatt cggtgctggc gttttcctgg
     5821 ctggaggaaa ttgcacgtgc tttgataccc ggacgaatgc gttgcaagcc ggaaacgatc
     5881 acccgatcgc cagcctgtaa gccagaggtg acaacccact ggtcgccgat ggctttgctg
     5941 gcttcgattt cgcgtagctg cacgacatcg tctttatcca gaatgagtgc cgttgcttta
     6001 ccctgggcgt tgtgggtgac gccttcctgc ggcaccagta atacattctg gcggctacct
     6061 tcatccacta atgccgtgac atacatgcca ggcagcaagt caccatttgg gttggggaaa
     6121 atcgcccgta acgtcacgga gcccgtggtt tcatccaccg tcgggtcgga gaatttcagc
     6181 gtgccggtct ggccgtaacg tttaccattt tccagattga gctgtactgg cgtactgccc
     6241 tgaacctgtt tgatttgccc actggcgacc tcttctttca tgcgcaagaa atcctgcacc
     6301 gactgcgtga gatcgacata aatcgggtcc agacgttgta cggtaaccag cgaatcagcc
     6361 tgattagcgg taacgagtgc gccgacggtc accgacgatt tcccgctgac gcccgtaatc
     6421 ggcgaggtga cattcgcgta ttgcagattg atcgtcgcct gttcaacagc cgctttggcg
     6481 acggtgacat tggcttctgc ttcattcaac tgggtgcgtg cggtgtcgta atcctgacgg
     6541 ctaacatagt tggtcttcag caacgatgcc tggcggttaa aggtgatgcg ggcattgctg
     6601 gcggtagaga gcgctttcgc cagcgaacct ttggcggagt tcagctctgc ctgtaaaggc
     6661 gcaggatcaa tctgatacag cgaatcgccc tggttcactt tatcgccttc gataaagttg
     6721 cgtttaatga taataccgcc cacctgggga cgtatctcgg caacttcata aggaacggtt
     6781 ctaccgggca attcgctcaa cacattgacc gaaccggggg agagtgtgac gacaccgacc
     6841 tcaggcgtca tggcggcggc gttttccgcc gatttgtcat cgcaggcggt gagcatcgcg
     6901 ccgcagaata acaacggtat taacagtttt cttcttctgt tcattttagt ccctgaaaat
     6961 tcttgagaaa taacggttct ttaaattgcg cgcgaggaga gatatgaggg atttacagac
     7021 cagcggcata gtggggttgt cagccagtaa gatgggatgc aagcacagtg atcgacatgg
     7081 tgaggtcaac gacaaaaaaa atgttgccgt tctgccaaca gtttctgcca gcattcgggc
     7141 aacgaagggt tctactggtg gatacacata ccaggggaat aaagtcgttt tcgtctctcc
     7201 ttcaataatc accgggggta ttgaaatcag catctgcatc cctcgtcatg ccagccatca
     7261 atttcagttg cttatgtcct tactaaaaat aagatgtgat acccagggtg acgatgtcgc
     7321 tcatacgtct taatttcagc ttgtacataa tgttgaattt ctgaatcttt aatgtttgtt
     7381 gcgaaatgtt gagcgttgac gtgatattgc tttcattttt gtttttcaga atcagttgca
     7441 taattttgaa ctgattatgt gaaaaacata gttgtgaatt tttatggggc aagtgtttac
     7501 cataagtaat ctcaagaaga tttttgatac tttctttacg gctgacaaaa taaataggcg
     7561 ctttagcttt tagtttactg atgtgataag gtgccaaaac aataattctc tcggcatcta
     7621 atttctccag gaatttaatc gcttcttcat caaggatatg gttgtgatag gtgtcaatga
     7681 tcaccttgaa gggtttattg cctacttttt tgatctctga cagggaattg atttttatca
     7741 tttcgtgatt atctttcaac tgccaaaagc cctgtaaaag aaaagaatct ttcgtcatga
     7801 gaaaaatcat aacttgctcc ttagccgtta tcgtttcgaa catgtcatcc ttttcattat
     7861 ttacatcctt gtccgaatcg ttgttcaata tagtaaacgc ctataagaac tttgcccttc
     7921 gtgaactaat acgtctatta acactaatca atacttcaga tttatccatc acacactcca
     7981 tatattggtc ctaagtaaga ttcctggttg ttatcagttt gtaactagta aacaaaaggt
     8041 taacttttgc ggattactgc ccaagaataa gttatcaata accagtttgt gatctctgaa
     8101 gaatattact aaagttaaaa tgtaatccga tttaaatatc gagtctcctt gtttcgactt
     8161 aggctggcaa ttggattgcc agctttcttt taacgcattg aaattacctg attaaataac
     8221 tcattcatgt cttcaggttt ttatatagcg tctggagaca cggatattta tgcaatgtaa
     8281 tcaacgccag ttgcaaaaaa atgaacccgg attacttatt taaccgccca tagcatcaac
     8341 gcttacggaa ctctttgttc actttcccac agcactttta aaacattaaa cctgaccacg
     8401 gaaaatatca gccatgataa tgtttgaaat ggctaatttg ccatggaacg aaaaaaatca
     8461 gattaaattc agtaggttga aataatcaac agcaggcaat tgtttcaatg atagtgcgta
     8521 attgaactga aacactgtat agctaagaga gtattaactg gatcatagtc acttaatgtg
     8581 actatgatcc aggtggaaat agggataatt attgcttttt aacgaacaaa ctggcgaagc
     8641 tgaacagact ggcggcgcta aatatcagtt caattcccac cagtgtggaa accagcgtta
     8701 ccgacaccat cggcgtagca ccaaggaata tccaggcaat gacgatatcc agcacaccaa
     8761 taacgagctg tagccaactg cctttcattg aacgctgacg ataccaactc atcaggcgaa
     8821 taacccctgc cacacagaac aaaccggcaa taaatgccgc aatggcaaaa atgcccagct
     8881 ctggtgcgcg gatgaagaaa tagccgatca ataaataggc gactgcgacg aggaaaccgg
     8941 ataataccgg ccagaaatta tgactgcggt tgctgaatag cccgacaata agcgcaatgc
     9001 ccgagcagat taataatgca cccactactg tgcttaaaat gtcgccagag acgaacggaa
     9061 aactgataca cagcaacccg acgataaaca gcagcacggc aataaactgg attgctctgc
     9121 gatgtttttt aagcatctcc agatcaaact tcaaaattgt tgccttatct atatataaca
     9181 tagaaccacc ctataaaatt aagaagaaaa tcccctgcta tcaatctatg ccaaaaacgc
     9241 gtgtaagaat gcagtcgatt taataaaaat ttcctaatta cagtatctga tgtatctgta
     9301 actcattgta ttgaaataaa aatatctgat tttgatattt tccatcaaca tgacatatac
     9361 agaaaaccag gttataacct cagtgtcgaa attgattcgt gacggctctt tcactttata
     9421 gttgaggata ttacgatgaa aaaagtatta ggcgttattc ttggtggtct gcttcttctg
     9481 ccagtagtga gcaatgcagc ggatgcacaa aaagcagctg ataacaaaaa accggtcaac
     9541 tcctggacct gtgaagattt cctggctgtg gacgaatcct tccagccaac tgcagttggt
     9601 tttgctgaag cactgaacaa caaagataaa ccagaagatg cggttttaga tgttcagggt
     9661 attgcaaccg taaccccagc tatcgttcag gcttgtactc aggataaaaa agccagcttt
     9721 aaagataaag ttaaaggcga atgggacaaa attaagaaag atatgtaatt cctggaatgc
     9781 gttacatcgt atttccttgc atattgaaca ggccggaata tcttctttaa aagcagctat
     9841 tcctcctgtt tgtatataat ctctatattg aatgggttac aaaatgaaca tttcatctct
     9901 ccgtaaagcg tttattttta tgggcgctgt agcggctttg tcactggtga acgcacaatc
     9961 tgcgctggca gccaatgaat ccgctaaaga tatgacctgc caggaattta ttgatctgaa
    10021 tccaaaagca atgacaccag ttgcgtggtg gatgctgcat gaagaaacag tatataaagg
    10081 tggcgatacc gttactttaa atgaaaccga tctcactcaa attcctaaag tgatcgaata
    10141 ctgcaagaaa aacccgcaga aaaatttgta taccttcaaa aatcaagcat ctaatgactt
    10201 gccgaattaa taaggtacaa gtaaaaagga gtagcaagtt gagccatctt gctgctcctt
    10261 tttgcatttt tatatgacag cagaatttat tatacgactt atacttgcgg taattgcctg
    10321 tggcgctatt ggcatggaaa ggcaaatgcg cggcaaagga gcagggttac gcacacatgt
    10381 attaattggc atgggaagcg ccctgtttat gattgtttcg aaatatggtt ttgctgacgt
    10441 gctgtcttta ggtcacgtcg gactggatcc cagccgtatt gctgctcagg ttgtgacggg
    10501 cgtcgggttt atcggtgcag gtaacattct cgttcgtaac caaaatattg taggtctgac
    10561 gacggcagcg gatatctggg tgaccgccgc cattggtatg gttattggca gcggtatgta
    10621 cgaactgggt atttatggtt cagtgatgac cttgctggtg ctggaagtct tccatcaatt
    10681 aaccttccgc ctgatgaaca aaaattacca tttacagctg acattagtga acggtaatac
    10741 ggtatcaatg ctcgactggt ttaagcagca aaaaataaaa acggatttag tttcgttgca
    10801 ggaaaatgaa gatcatgaag tggtcgccat tgatatccag cttcatgcca caacgtcgat
    10861 agaagatctg ttgcgattat taaaaggtat ggcaggggtg aaaggcgttt caattagcta
    10921 atctgtatag ttatttattc gcaggagtac cagtgcggta ctcctgttgg ttttaatgat
    10981 cgaggacgat cagtggtgaa tggttggcag gatctttaag tacggtaata tccgcaccgt
    11041 agagcattgt cagcgcctgc tgtgtcaata cgtcctgcgg cttaccattt gcgataacct
    11101 ttcctttttg cattaataca acacggtctg cataacgcgc ggcaagattt agatcgtgca
    11161 atacgcagca aacattgaac tgccgttcgt gaactaactg gcgtaataaa cgaaacaagt
    11221 gctgctgatg gtgaatatcc agcgccgaag ttggttcatc aagaaataac catttcggtg
    11281 agggagtagg ttcccagagt tgcaccagca ggcgcgctaa ttgcacgcgc tgttgttcac
    11341 caccggataa ttgccgatag tcgcggtttg ctaacgcctg acagtcacat aacgccatta
    11401 tttgtgctgt ttcattatcc tgattaccag tacgatgcgg atgccgcccc atctggataa
    11461 cttcttgtac gctgaatgga aacgccatgt ggctattttg acgcatcacg gcccggtgtt
    11521 tcgccagttc agtaatcgac cattcattta atggcttatt aaacaaccga cattcgccgc
    11581 tatcaggttg aagatagccg gttagctggc gaagcagcgt agattttccc gcaccattag
    11641 gcccgaggat ggcaacaatt tccccaccgg gaaaagtcag cgagacatta tcagtcagac
    11701 gccgcccctg aaggctataa accagattct gggcgctgat catgtgatcc tccggttgcg
    11761 cagaatcaac cacataaaat aagggccacc aagcaggctg gttaataatc ccacgggcat
    11821 ttctgctggc tgtaccagcg tgcgggcaag cgtgtctgcc atcagcaata aacaggctcc
    11881 ggcgagggcg gaacaaggga ttagccagcg gtgatttgcc ccggtagtca tgcgaatcag
    11941 atgcgggatc accaggccaa taaagccgat aatgccgctt accgatacgg cagcaccaac
    12001 gagcagtgag ctcactaaca gtaattgctg gcgtttacgc ttcacgttca cgccgaggta
    12061 gtgggcttct tcatcaccga gctgcagtaa attcagcgtt ccggcgagac aagttgttgc
    12121 gataatggcc ggtaggatga atgaactggc aaccaataac gtcggccatt gcgcctgtcc
    12181 aagattgccc atcatccaca atgtgagttg tcgtagctgc tgctcatcgc cgatatagct
    12241 caggatgccg atcgctgcgc cgcacagggc attgatggcg ataccggcga gtaataattg
    12301 catcatgctg ccatcgcgat ggcgctgcgt gatgagaaaa atgatggtgc agaccactaa
    12361 acttccggcg atggcaaaca ccatttgctc ataaagcacc agcaccactg ggaaggagaa
    12421 gggcagcaca atagcaacgc caaccattaa tgcagaaccg ctactgacgc caagtaatcc
    12481 agggtcagcc attgggttgc gaaaaagccc ttgcataatt acccctgcgg ttgccaacgc
    12541 tgcacctacc agcaccgcca gcaaaaccct tggtagacga atattgagcc agatatcgcg
    12601 gtattcctca tcaaaaaggg catcaaaact gatctttaac gctccctgac tggcaccaaa
    12661 cagcaccaga gcgagcaata atagtgatag ccccataaag accctggcgg aagagagtga
    12721 atccttgagc acgcaaaact ccgttacgac attacggcgc ggacatgccg cgccggggga
    12781 tcacgccggt ttcaggtccg gttcaatcag actgtagacc tgctggttaa gtgctggcgc
    12841 atttgccagt tcatggatat gtgcggcgac atcagcacgg ttaacaaaac catgacactc
    12901 ctgattttgt atccgttgtg ctttgcccgt tgccgcacca tcaagcagac cgccagggcg
    12961 gagaatggca taatcaagct ggctggtttg caaccagctt tccgccagcg ttttttcccg
    13021 taccgcctga ccaaacgcag cctttgcccg ctctgataaa aacggccagc tatcgccgca
    13081 tcccaaagag gtcaccagaa tcatgcgact gatgccagcc ttctcggcct catcaatcac
    13141 tgttctgtgg gccagataat cctgagctcc gcccattgtt gagatgataa gcgcatccgg
    13201 gccagctgcg cggcaagcag ccgcgactac gctggcatca caggcgtcgc ctgtaaaaac
    13261 ctgtacgcct tgttgcgcca gtttcgtggc ggcatcggca tgacgaatga cagcaacgac
    13321 cggacgttgt tccgccagcg ccagttcaag cgttctggca cccacacctt ttccgcctgc
    13381 gccaaagagt agccacggtg tcatcaggcg tgttccttta acgatgctgc cagagcatgg
    13441 aaggcgctga cttgttcgct caggagctga cgatgatcat ccctaccgag gaagattttc
    13501 agcattgcgc tgccttcctt attgaaaaag agaatagagg cggtgtccat gcccataaat
    13561 ttacgttcga tcaaggcaat atgcgtgcag ttttctgctt tgatatgccc ggacatgccg
    13621 tgctttccgc gtaggttgaa atagccgtgg cgatggaaac ccgaaggcag ttcgccgcta
    13681 aattcgagta ttacatcggt agtatgtact aacgtggtga cgttgcccca ttcgcagacg
    13741 gtatcccaga cagtatcgaa tttatcacct gacacaacag tcggagaggg cagatttttc
    13801 actacctcca gcagcgtggt gttgtattgt tcggcgatcg cctccagcgt accgtctggc
    13861 tccgttttca aaaattcctg taacgagaca tggctcataa atcactcctg ttatggtttt
    13921 tcaagcgcaa ttgctggcgc attaagtacc tgaatcagtt cattaagaga ctgcaaaatg
    13981 ttgcttgccc agaaacgacc ttcattggtc aaccgcagac acgttgacgc atcgcggctt
    14041 aagccttttt ggtgccactg ggccagtaac ggcgcgagtt tttcggcatg tggcgtaagt
    14101 tcgtccagcg gtacacgcgc cgtttcaatg cccgactgca aggtatgacg ccactgcgcg
    14161 ttacgttcgg cgttacgcat gatcatcatc agcggttttt tgcctgctgc gacggattca
    14221 tgccaggtct gaagattgcg ttcgttcatc caggagtaac cattaatcga accgccggct
    14281 ccggaaccaa aggccagaca atcggcacct tgtttaatca gcaggttata gagattgcgt
    14341 tcgcgtgtgg tacggcccca gtggctgtta ctgatgcagc gccaaccggc atcgtccata
    14401 aaatcacatc cttgcaggta aagatcgcga cgttctgccg gagagggcac ggtagtacgc
    14461 ccattttcca cggctttgcc cagcggtgta ttgggcagga cattgagcgc atagagatcg
    14521 acgccgtcga gaccgatatc gcgggcaata gccagatctt ctccccaggt ttgcgcatcc
    14581 tgacccggca gaccaaacag caggtcacag accaccgcgg cacggtcgcg tttgaccagg
    14641 ctttccataa acgcaatagc ggttgggcca tccgaggtgc gggccatttt cttgcggatt
    14701 ttgctgttaa acgactgaat accaattgag aagcggtttg ctccagcatc aaggcaagca
    14761 tcgattcgct cagcgtcaaa attcagtacc cggccttcga tagtgatttc acaatcaggg
    14821 gccagtggca gcttttctcg taatgtggtg atgattctgg ccaaatcgtg tgctgaaagc
    14881 gcggaaggcg taccgccacc gaaatagacc gcgtggatgg ggcccgactg atgcaatacg
    14941 ctgtctgctt ccagttctat ttcacgaatc agggcgtcgg tataatgagc acacgcatct
    15001 tcattaaaac ggttctgata aaaaccacaa aacgtgcagt gtgtcgcaca aaaaggaatg
    15061 tgtaggtaaa ccagtcgctt acgtggtgat accgtttgat tgatcatctc ttgccaggtc
    15121 tgtgccagtt gctctttggc aaccggaatg gccccacgaa acggcatcat ggcgcgcctg
    15181 tctttaaagg gctgatcacc gtctaaagca aaatgtggcg tgagatccag ggtgttattt
    15241 gtgttcattc gtctgattta aatccattta ttgcggccaa agctgctggt gtaaagactc
    15301 aacgacatca gcaatacgcg gacccattcc cagaatcagg ttttgatcaa cggtgataat
    15361 gcgctggttt ttccatgcgg cagtgtgggt aattccggca atagaacgta gccgattaat
    15421 atcgccatcg accatttgtg aggtgacgac aatcacttca gggttagccg caatcagcga
    15481 ttccgcgctg tagcttttgt actgctggtg ggtagcgacg ttttctgctc cggcaagcga
    15541 aagaatggcg tccgcgacgc tgcctttacc ggcaacctgt ggcgcacttc cgcctgcgga
    15601 gagaataaac atggctttaa ccggggcttt tttggccgcc acgttttgct gtactcgctc
    15661 caggcgttgg ttgatctgtg tcaccagcgc ctcgccttgt tcaggaacct gtaacgtttt
    15721 tgccagttgg cgaatgttgg cgtacatctg ctcaagggtg gctggtacac gcggcagagt
    15781 gacgacgttg actttttgcg cccgcagttg gtcgagcaca atttgcggtc ctgcatcctg
    15841 ccaggtaatt acgctatccg ggcgaagtga caaaatgcct tcgctgctga gttgtttcca
    15901 gtaaccaata tgcggcagtt tggcggtttc tggtggatag gatgtcgttt catcgacacc
    15961 aaccacgcgt ttgccagcgc ccatcgcgta gattagctcc gtcagcgatc ctcctgcgac
    16021 cacgatacgt tcggcagccg ttacgctaaa agtccagcta agagccagta atgccgtaaa
    16081 caattttttt cgttttacca tggtcttcgc ggcagagacc acggctgaaa tacataacgt
    16141 tagtggggaa aaaagaaacg gcctggtgat gatccttggc ataatgtgag aactcttatc
    16201 aaattgagaa tgatcatgat tatcatttga gttatatatg aaatacaacc gtcgattcaa
    16261 tacgcacggc gttacaagcg tatttatttt tgatttttat caagatgctt cgtcagctaa
    16321 tgttcttgtt accgattaaa tttcagcgcg ggaaaaagct gagaacccgg agatatcgcg
    16381 gcttgcagag caaagagaag agaaaattat cactgttaac gacaacgttt tgtgatgagg
    16441 ttgtgggggg gatagccata aacacaggat ggttttctgg atgctggata gatttcataa
    16501 ccccctgttt attgtgttaa tggcggttaa aaaaagcttg atcataaaaa attgatcgat
    16561 aataattctc attatcacgt ttgagtttaa caaaatatga catcatggat aacaaaatca
    16621 tatcttttgt aagggaaaac gcacagcact cttcacttca gacgtggcgt actactttgc
    16681 ctgacaaaga gggcgtgctt ttgctgaatt atcagaaata ttcggcaatt ttacgggata
    16741 tatacgctaa tagcttcccg tggtgatatc taatcatttg aattgtaaat aaatttattt
    16801 ttcgccagcc accccggtat taacccaggc agccagcatt ttttattttc ctcatggaga
    16861 atcgctatgt cacgtccgca atttacttcg ttgcgtttga gtttgttggc tttggctgtt
    16921 tctgccacct tgccaacgtt tgcttttgct actgaaacca tgaccgttac ggcaacgggg
    16981 aatgcacgta gttccttcga agcgcctatg atggtcagcg ttatcgacac ttccgctcct
    17041 gaaaatcaaa ctgctacttc agccactgat ttgctgcgtc atgttcctgg aattactctt
    17101 gatggtaccg gacgaaccaa cggtcaggat gtaaatatgc gtggctatga tcatcgcggc
    17161 gtgctggttc ttgtcgatgg tgttcgccag ggaacggata ccggacacct gaatggcact
    17221 tttctcgatc cggcgctgat caagcgtgtt gagattgttc gcggaccttc agcattactg
    17281 tatggcagtg gcgcgctggg tggagtgatc tcctacgata cggtcgatgc aaaagattta
    17341 ttgcaggaag gacaaagcag tggttttcgt gtctttggta ctggcggcac gggggaccat
    17401 agcctgggat taggcgcgag cgcgtttggg cgaactgaaa atctggatgg tattgtggcc
    17461 tggtccagtc gcgatcgggg tgatttacgc cagagcaatg gtgaaaccgc gccgaatgac
    17521 gaatccatta ataacatgct ggcgaaaggg acctggcaaa ttgattcagc ccagtctctg
    17581 agcggtttag tgcgttacta caacaacgac gcgcgtgaac caaaaaatcc gcagactgtt
    17641 gaagcttctg atagcagcaa cccgatggtc gatcgttcaa caattcaacg cgatgcgcag
    17701 ctttcttata aactcgcccc gcagggtaac gactggttaa atgcagatgc aaaaatatac
    17761 tggtcggaag tccgtattaa tgcgcaaaac acggggagtt caggcgagta tcgtgaacag
    17821 ataacaaaag gagcaaggct ggagaaccgt tccactctat ttgccgacag tttcgcttct
    17881 cacttactga catatggcgg tgagtattat cgtcaggaac aacatccggg tggcgcgacg
    17941 acgggcttcc cgcaagcaaa aatcgatttt agctctggct ggctacaaga tgagatcacc
    18001 ttacgcgatc tgccgattac cctgcttggc ggaacccgct atgacagtta tcgcggtagc
    18061 agcgacggct acaaagatgt tgatgccgac aaatggtcat ctcgtgcggg gatgactatc
    18121 aacccgacca actggctgat gttatttggc tcatatgctc aggcattccg cgccccgacg
    18181 atgggcgaaa tgtataatga ttctaaacac ttctcgattg gtcgcttcta taccaactat
    18241 tgggtgccaa acccgaactt acgtccggaa actaacgaaa ctcaggagta cggttttggg
    18301 ctacgttttg atgacctgat gttgtccaat gatgctctgg aatttaaagc cagctacttt
    18361 gataccaaag cgaaagatta tatctccacg accgtcgatt tcgcggcggc gacaactatg
    18421 tcgtataacg tcccgaacgc caaaatctgg ggctgggatg tgatgacgaa atataccact
    18481 gatctgttta gccttgatgt ggcctataac cgtacccgcg gcaaagacac cgataccggg
    18541 gaatatatct ccagcattaa cccggatacc gttaccagta ccctgaatat tccgatcgct
    18601 cacagtggct tctctgttgg ttgggtcggt acgtttgccg atcgctcaac acatatcagc
    18661 agcagctaca gcaaacaacc tggctatggt gtgaatgatt tctacgtcag ttatcaagga
    18721 caacaggcgc tcaaaggcat gaccactact ctggtattgg gcaacgcctt cgataaagag
    18781 tactggtcgc cgcaaggcat cccacaggat ggtcgtaacg gaaaaatttt cgtgagttat
    18841 caatggtaat catctgcccc gatatttcgg ggcatttatc tggaaggaag agagacaatg
    18901 aaccactaca cacgctggct tgagttaaaa gaacaaaatc ccggaaagta cgcgcgtgac
    18961 atcgcaggtt taatgaatat cagcgaagca gaactggcat ttgcgcgcgt cacgcacgac
    19021 gcgtggcgga tgcgcggcga tattcgtgaa attctggcgg cgctcgaaag tgttggcgaa
    19081 accaaatgta tttgccgtaa tgaatatgca gtccatgagc aagttggtgc gttcacaaac
    19141 cagcatttga acggacatgc cggattgatc ctcaatccgc gcgcgctgga tttacgtctg
    19201 tttctcaatc aatgggccag tgttttccac atcaaagaaa acacggcccg tggcgaacgc
    19261 cagagtattc agttctttga tcatcagggc gatgcattac taaaagttta tgccaccgac
    19321 aataccgata tggcggcatg gagtgagctt ctggcacggt ttatcaccga tgagaatacg
    19381 ccgcttgagt taaaagccgt tgatgcgcca gttgttcaaa cgcgagccga tgccagtgtg
    19441 gtcgagcaag agtggcgggc gatgaccgac gttcatcagt tttttacgtt gctcaagcgt
    19501 cacaatctga cgcgccaaca ggcgttcaat ctggtggcag acgatttggc ctgcaaagta
    19561 tccaacagtg cgttggcgca aattcttgaa tctgcacagc aggatggcaa tgaaatcatg
    19621 gtgtttgttg gcaaccgtgg ctgcgtacag attttcaccg gtgtggtaga aaaagtggtg
    19681 ccaatgaaag gttggctgaa tattttcaac ccgacgttta ctcttcatct attagaagag
    19741 agcattgctg aagcctgggt tacccgtaaa ccgaccagcg atggtcacgt aaccagtctg
    19801 gaattgtttg cccatgatgg tacgcagata gcgcaacttt atggtcaacg tacagaaggt
    19861 gaacaggagc aagcgcaatg gcgtaagcaa attgcttcgt tgataccgga aggcgttact
    19921 gcataacgta taattaacgg gaggtctggc tgttgctggg cctctttata ttgatcaatc
    19981 gttcacacca gataattaat atgctgatgg cgtaccagct cattgatccg cttaagattg
    20041 aggatcatca tgatattgtg tttatggcag tagaacgttt ttagcgatat tttcagtttc
    20101 tccagaatat cttccgttgt catttgattc gcaatacagc tcagaacctg atgctgcttg
    20161 cgcgagaggg acacatccgc ttcaggcaca gagcagtaac tgccattgat gagtgcgaca
    20221 aagtcgatga tggcgatagt tcgcggaaca aaggtcaccg gtactttccc caggcagcgt
    20281 ttaatattaa agggggagaa aataattaca tggacaggtt ttaatcgttc aatttgagtg
    20341 agaaaattag aatgaaatac attattcatt aatgtatcaa taatgacgaa ggcattctga
    20401 tgcaacatta cgtcgatctc ttcttcgttc tgtatatgaa cgacattacc tttactcaga
    20461 atgtttttca tcgcggtgaa aaacatcgta tccctggtaa ttataagaaa cataaccgac
    20521 tcctggtacg gacattaact ttataatcag tttgccaaag gtaattaaca ggacagggct
    20581 tataataata gttagcttac tgtgtattaa tttgatgtaa tgcatgatgc agatgctgag
    20641 aaagcatctg catctttcgg tggtgttatt tgaccagctc aggtgttacc tgactcaccg
    20701 cattggtgta gtaaggagcc ccccagcccg gttccggcca gaatgcacca tagccgtaat
    20761 cccacaggtt ataggtggta ttaaccactt ctctcaaatg ccacacctgg atgccctgca
    20821 tattcacttc gaggaagtta tacgggactt tattgataaa gccaggttgt tcaccctgaa
    20881 tggtaccgag gatggtaaca aagtgattac gatagttcac tggatcaagg aagccgcttt
    20941 gtctggcgag cagtcggccc tgatagttgg cttcaatatc aggcttcgca tagctatcca
    21001 acggtaatac agcgatttct aacaacgtat ccgttttgcc attgataacg ttgataacct
    21061 tcccaccaaa gcgcgcttgt tgaccaacat ataaccccgg ctggttatga acagcaacaa
    21121 aacttttttg aatatcaggt tggttattgc ctttgatatt ttgcggaatt gaactacatg
    21181 cggcaagcaa aaatgaaagg ctgaggatga gtgcaccttt tgtcatgttc atgttactat
    21241 ccttatcaac aaatattaaa accataatag atatttcttt atagttttca tctgattctg
    21301 aggcgatatg cctgatagta acgtaaacct gtctgatgtt tatatctctt ctgtttagtt
    21361 gagctcagtt aatttttggg cgtggcatga gtatccattg ttccataatt tatattagga
    21421 ggtttttaat cttttcgtgt ctaatgttat ttatctgatt aagtattgat tgtgtctttt
    21481 gcggaagtgg tgtcgagctt atcgtttcaa tgttattaat aagacttaca agttgagcct
    21541 caactttgag tagtctgcat ttgtatatgt ggtgttcgta tggcacgcat taatttgttg
    21601 gcgttgttgt tgcgctaaat caaattgttt agaggtgtat ccttctccag agaatgggga
    21661 attaatgggt tgttgttctt cccggttatc acaaggccac ttcggtgtgt ctggcgtttt
    21721 aacattatcg gtatttgttg tgcagttata aatatcaccg ttatatgtat ttattacacc
    21781 tggggtaaag tcaattgaca taacattcct tattcatcat ttgttttatg ctaagatttt
    21841 aaaattggat aattagaaca ggaatggatt ttcttctaca tacagtaaat ataatgcgtc
    21901 atatcttcct gatataatta cctttgactt ctcttttatt tccatctggt aatatttgag
    21961 tgcaaaaaaa ggcaataata atatcgcaaa agaaaacgtt aacaactatc gaaaatgctc
    22021 gttttaaaaa ggtatgatgt tctctgatag tgtgtgaagt gatatttttg tgatgtttta
    22081 gatatgtaat aataaaatgg gttaacgtat gttgaaatta tttattgtgt aaaccactga
    22141 tagatcataa tccagtacat ctttacagtg ctgcactgta tcgctgataa ctaagcagct
    22201 cactgataca ccatgctgga cgcgataatt ggaatctcac gcagggagta ctgtgtaaat
    22261 agcaaaatcg cctgatgcgc tacgactgtc aggcctacaa ataacataca attcatcgaa
    22321 ttatcataac gttgtaggtc ggataagacg cgtcagcgtc gcatccgaca taaataaagc
    22381 gcatttttct aacaacctgt gggggaatat agccgcttta tttcaggcga ttacccgcct
    22441 catcaaccac tttctcgcca tcttccttgg tgaacgcgcc tttttgcgca tctggcagaa
    22501 tttccagcac gacttctgaa gggcggcaca ggcgagttcc ctgtggcgtc accacaatcg
    22561 ggcgattaat cagaatcggg tgctgaagca taaagtcgat taactgatcg tgagtaaatt
    22621 tatcttctgc aaggcccagt tcctcatacg gctcgacgtt tttacgcagc aacgcgcgta
    22681 cggtaatccc catatcggca atgagtttta ccagctcatc gcgcgtcggt ggtgtttcca
    22741 gataatagat aatggtcggt tctgtaccgc tgttgcggat catctccagc gcattacgcg
    22801 acgtaccgca ggccgggttg tgataaatgg taatgttgct catatcagta tctcattaca
    22861 atgtgaaaga gaggtgtagc gccagcgcca ccagcgttac aaaccaaagc ggacaagtca
    22921 aaaacacata tggtttaacg aatatgtaag tgtagagaaa ggtgtaggtg tgaattaagc
    22981 gttgggcatt catttgaaag gaggtctgaa tcaggacgta aaaaaaccga cttcgcgtcg
    23041 gtttttttac tgttcagccc tgagttggtg gctctggctg gagtgagata gcccacatct
    23101 aacaatgaga catgctccgt gacaggcaat ggatgagtaa gcggatgcat tctcactcca
    23161 tcgcatggag aaaacgggtg attgataaag caatcatcgt tcttggggcg ttaattgcgc
    23221 agctggaact gatccgcttt ctgcttcagc ttctgaactg atagcggaaa cgtagctaag
    23281 ggctaagagc acactactct tagcccttta acatttaacg cattgtcacg aactcttctg
    23341 ctgctgtcgg gtgaatggca accgtgttgt cgaagtcttt tttggttgca cccatcttca
    23401 gcgccaccgc gaagccctgc aacatttcgt ccataccaaa accaatgccg tgaataccga
    23461 caatcttctc ttccggtcca atgcatacca gtttcatgcg acacggctgg cggtgagtgg
    23521 tgacggcggt atacatcgcg gtgaaagagg atttatacac tttcacctga tcgtcgccgt
    23581 actgctcgcg cgcctgcggc tccgttaaac caacagtacc aatcggcgga tggctgaaga
    23641 ccacggtcgg aatgttgctg taatccagat gctcatccgg cttgttatta aacaggcgtt
    23701 cagagagacg gcgacctgct gcaactgcca ccggagtcag ttccactgca cccgtgttat
    23761 cgcccaccgc gtaaatacct tcaacgttgg tgttttgata tttatcgacg acgatatagc
    23821 ctttttcgtt ggttttaacg ccagcggctt ccaggttgat gttgtcattg gcaggctcgc
    23881 gaccaatcgc ccaaatcagg caatccaccg tttcactgcg accatcttcc agctccagcg
    23941 tcaggctgcc gtcggcattt ttcaccaccg ctttcgggat ggcgttggta tgcaactgtg
    24001 ggccttcggc gttcatcact tcgaccagcg tttcagagat catcgggtcg aagctgcgca
    24061 gcggcgcatg tttacgcaca aacagatgcg tcttcgcacc gaggccgtta atcacgcccg
    24121 ccagttcaac ggcgatgtaa cccgcgccaa caaccgctac gcgctctggc aaagccggaa
    24181 gggcgaagaa gccatcagaa tcaataccgt attccacgcc cggaatatcc gggtggctcg
    24241 gacgaccacc ggtggcgatc agaatatgat cggccgtgat ggtttcgccg ttgacttcaa
    24301 tggttttggc atcaacgaag cgggcaaagc ctttgattac atcaacgtta tttttaccga
    24361 gcacgttctc ataggaagta tgaatacggt cgatataggc ggtacggctg gcgatcaatg
    24421 tttcccagtt gaatttattg atagtggtat caaagccgta atccgggccg tacatatgga
    24481 tcgcttcacg gatttgcgcc gcgtgccaca tcactttttt cggcacacag ccaacattta
    24541 cgcaggtgcc gcccagctct ttggcttcaa tcagtgcgca tttctggccg tacatagccg
    24601 cgcggttgat ggaggcgata ccgccgctgc cgccgccgat ggcgatgtaa tcatagtgtt
    24661 tagtcatgac atattgtcct tatcgttgat taccgcgatt gtagcgcgag acgtaatagg
    24721 tgccagcaat ggctgcaatt actctggcac gatccagctt acggtggcgt gcccggtgcc
    24781 tgccggaacc agtttgctgt gcagccacgg cagcacgtta ttcatctgct gttccagctt
    24841 ccacggcggg ttaatcacaa tcatgccgga agcggtcatg ccacggcgat cgctgtctgg
    24901 cagcaccgcc agttcaattt gcagaatttt gcgaatgccg gtcgcttcca ggtcgtggat
    24961 catgcgctta atttgctggc gcagcaccac tggataccac aatgcgtaag tgccagtagc
    25021 gaaacgtttg taaccttctg ctatcccgct gaccaccgcc tgatagtcgg ttttcatttc
    25081 atacggcggg tcgataagga ttaaaccacg gcgggaaacc ggcggcagct tggccttaag
    25141 ctgctggaaa ccgtcggctt tttcgacgcg cgcacggctg tctttctgaa attcagaacg
    25201 cagcaacggg taatcgctcg ggtgcagttc ggtcagttgc aggctgtcct gttcacgcag
    25261 tagctggcgc gcaatcaacg gcgaaccggg gtaataacgc aactgcccgc tacggttgaa
    25321 gtgttttacc acattgatgt acgcctccag ctccgcaggc aaatcgtcct gctgccagat
    25381 acgggcgata ccttcgagat attcgccggt acgctcggca tgttcgctgc ctaactgata
    25441 acgcccggcc cctgcgtggg tgtcgagata gagaaacggt ttatctttct ctttcagcga
    25501 ctcgatgatc aggctctgaa cggtatgttt aaggacgtcg gcgtggttgc cagcgtgaaa
    25561 gctgtggcga taactgagca tgggtaaagg tgttccggta agtaaaaatc ggcccgtatt
    25621 cggggcgcac aaaagcctat caggacagta taccgaaaag aggccgccgc cgcgaaagcg
    25681 taacgtttct cattgaaatt cactacactt aaccccatgc tacaaacatt atgtaaagcg
    25741 cctgttgagc gcttccttaa cctctttaac caggactgcg ctaatgacga atccgttact
    25801 gactcccttt gaattgcctc cgttttctaa aattctcccg gaacatgtcg ttccagccgt
    25861 gactaaggcg ctgaacgact gccgcgaaaa tgtggagcgc gtagtagcgc aaggggcacc
    25921 gtacacctgg gaaaatctct gccagccgct ggcggaagtg gacgatgtgc tggggcgtat
    25981 cttctccccg gtcagccatc tgaactcggt gaaaaatagc ccggaactgc gtgaagcgta
    26041 cgaacaaacc ctgccgctgc tgtcagaata cagcacctgg gtagggcaac atgaagggct
    26101 gtataaagca tatcgcgacc tgcgcgatgg cgatcattac gccacgctga acacggcgca
    26161 gaaaaaagcg gttgataacg cactgcgcga cttcgaactc tctggcatcg gtctgccgaa
    26221 agagaaacag cagcgttacg gcgaaattgc cactcgtctt tctgaactgg gcaatcagta
    26281 cagcaacaac gtcctcgatg cgacgatggg ctggaccaaa ctcgttaccg acgaagcgga
    26341 gctggcgggg atgccagaaa gcgcgctggc tgcggcaaaa gcccaggccg aagcgaaaga
    26401 gctggaaggt tatttgctga cgctggatat cccaagctac ctgccggtaa tgacctactg
    26461 cgacaaccag gccctgcgtg aagagatgta tcgtgcttat agcactcgtg cttccgatca
    26521 aggtccgaac gccggtaagt gggataacag caaggtgatg gaagagatcc tcgcgctgcg
    26581 tcacgaactg gcgcaactgc tgggctttga aaactacgcc ttcaaatcac ttgccactaa
    26641 aatggcagaa aacccgcagc aggtgctgga tttcttaacc gatctggcaa aacgcgcgcg
    26701 cccgcaaggc gaaaaagagc tggcacaact gcgcgccttc gccaaagccg aatttggcgt
    26761 cgatgagttg caaccgtggg atatcgctta ctacagcgaa aaacagaaac agcacctcta
    26821 cagcatcagc gatgaacaac tgcgtccgta cttcccggaa aacaaagcgg ttaacggcct
    26881 gtttgaagtg gtgaaacgta tttacggcat caccgctaaa gagcgaaaag atgttgatgt
    26941 ctggcatccg gatgtacgtt tcttcgagct gtatgacgag aacaacgaac tgcgcggcag
    27001 cttctacctc gacctgtatg cccgtgaaaa caaacgcggc ggggcgtgga tggatgactg
    27061 cgtaggccag atgcgtaaag ccgacggttc gctgcaaaaa ccggtcgcgt atctgacctg
    27121 caacttcaac cgcccggtaa atggtaaacc ggcgctgttt acccatgacg aagtgatcac
    27181 cctgttccac gagttcggtc acggcctgca ccatatgctg acccgcattg aaaccgctgg
    27241 tgtttccggg atcagtgggg tgccgtggga tgcggtcgaa ctgccgagcc agtttatgga
    27301 aaactggtgc tgggagccgg aggcgctggc gtttatctcc ggtcactatg aaaccggcga
    27361 accactgccg aaagagttgc tggataaaat gctggcggcg aagaactacc aggcggcgct
    27421 gtttatcctg cgccagctgg agttcggcct gttcgatttc cgcctccatg ccgagttccg
    27481 tccggatcag ggggcgaaaa tcctcgaaac tctggcagaa atcaagaaac tggttgccgt
    27541 agtaccgtct ccgtcatggg gccgtttccc gcatgctttc agccacattt ttgccggtgg
    27601 ttatgcggca ggttactaca gctacctgtg ggccgatgtg ctggcagcag atgccttctc
    27661 gcgttttgag gaagagggca ttttcaaccg tgaaactggg cagtcgttcc tcgacaacat
    27721 tctgagccgt ggcggttcag aagagccgat ggagctgttc aaacgcttcc gtggtcgtga
    27781 accacagctg gatgcgatgc tggagcatta cggcattaag ggctgaacat tcattgaaaa
    27841 tctgcttaat tgatgaaaca ggcgccggag acggcgcctt atctgttctg gcggcccgct
    27901 gggggctgga acacgatgaa gacaacctga tggcgctggt gttaacgccg gagcatctgg
    27961 aactgcgcaa gcgtgatgag ccgaaacttg gcggcatctt tgttgatttt gttggcggag
    28021 cgatggcgca ccgacgcaaa ttcggcggcg gacgtggtga agcggtggca aaagcagtgg
    28081 gcattaaagg cgattatttg ccggatgtgg tggacgccac cgcaggatta gggcgcgatg
    28141 cctttgtgct ggcttcggtc ggctgccgcg tgcggatgct ggagcgtaat ccggtggttg
    28201 ccgcgttgct cgacgatggt ctggcgcgtg gttatgcgga tgcggaaatc ggcggctggt
    28261 tacaggaacg tttgcagttg attcacgcct ccagtctgac ggcgctgagc gatatcaccc
    28321 cgcgtccgca ggtagtttat cttgacccaa tgttcccgca taagcagaaa agcgcgctgg
    28381 tgaaaaaaga gatgcgcgtg tttcagtcgc tggtggggcc ggatcttgat gccgatggat
    28441 tgttggagcc tgcgcggttg ctggcaacca aacgcgtggt ggtgaagcgt ccggactatg
    28501 cgccgccgct ggcgaatgtc gccacgccaa acgcggtagt cactaaaggg catcgctttg
    28561 atatttatgc cggaacgccg gtgtaaaagt aaagcggggt agcgataacg ctaccccgct
    28621 ttctgcaaga ataattaatg gctttccggc gtcgcaatca tgcgtttcag ccacggcacc
    28681 atcaatagca tcactactgc aacgcccagc gtaaccaggc caatcttacc aaacacgttg
    28741 gtatagacgg gcaacgtctc aagtggatcg gtaatgttgt ctggtactgc ggtaaatgtt
    28801 gccacatagc cgcccagcaa gaacgcggca gcctgcgtca ggaaccacat cccgagaata
    28861 aagcccatca aatgctgcgg caccagggca gcaatcatcg ccaggccaag ggcgctaata
    28921 aacagttcac ctaagctctg gaataagtac accagcacga taaaccatgg cgatgtcagc
    28981 ccctgggcat ccgcaaacca cattccagcc gctgcagccg tcaaaaagcc cagtgagcac
    29041 ataaacatgc cgagagtaaa cttcatcggc atcgagagat ctttgccttt gttacccaga
    29101 tgcgtgtaaa tgcctgccag tattgggctg gcgagtacca cccagaacgg gttaagcgcc
    29161 tggaagctga ccgggttgat ggaaaagccg agaatttcat gatgcacgtt gttgatggca
    29221 aagaagttca gcgacgttgg catctgggcg tagagaatgt aaaacaccac cgcttcgagc
    29281 atcaggacaa aggcgacaaa cattttattg cgcccggttt tatccagctt gaatgcctga
    29341 cgaaagaaga tgatggtgac gacgatggag agaacaatca gcaccagatt ggcgacttct
    29401 acgttgtgca tcagccaggc gcagacgaag atcatcacca cgctgccaag taatacgtac
    29461 aacagtttac tgaagctcat cggccggaag tcgggttcag aaccaatatc tttcaccatt
    29521 ccacgacagg cgatgtaaac cagtaatgcg ataattaacc ctgcaccgca caggttgtag
    29581 gtgactgaat agccgaattt atcagcgatc acaggggcca gcgataacgc tatcaacgag
    29641 ccgatattga tcgacatata gaacagggtg aatgcgccat caagccgtgg atctttcggc
    29701 ggatagcatt tcgaaagcaa gctggctggg ttagctttaa acaggccgtt accgacagcg
    29761 atagtcccca gggcgatgaa aatcaggtca ggcttaagta gcgacatgcc ggtcatgaag
    29821 tagccaatcg ccagcacaag tgctccgaga acaatggtgc gtttggttcc cagcaggtgg
    29881 tcgccaacat agccaccaat ggaaatgagg ccatagacca gcgcagcaaa agcaccaaaa
    29941 gtgacaaaag cctgctcttg cgagaaccca agctgtttaa cgaagaaaac cgccagtacg
    30001 ccctgcacgc cgtagtagcc gaatcgctcc cataactcga caaaaaagat catgaaaaat
    30061 gggcgaggtt gctgcagcat ccccatgggt gttgttgtat tcatatttat taaccttcca
    30121 ataccatcct gaaaagtatg aacgcgtaag ggataacgcg cgaaaaagtt tacatctgtc
    30181 agagattctt aatattgatg cggcgaatag attaatacac tattacaaca gaaaataacc
    30241 agctgatcac actaatgata gaaaatatga tggtgttaaa tgttgtattg gcaggggttt
    30301 taattgcgta cgcagaccgc aggccagata aggcgtttac gctgcatcag gtaacaaggc
    30361 gcgccaaaaa gcaaaacgcc cgcatatgcg ggcgttcagg aacacgtcgt ggagggaaga
    30421 ttattcttct tcgtcgcgca gcggaacaat cagcatatca acgtgaacgg tgttgatcag
    30481 ctgacgtgcg gaagacatca gtttgctcca gaagtcctgg tggtgaccac aaaccaccag
    30541 atccatatcg tatttcttga ttgcatcgac cagtacctgg cccagatcgc cgctgccgct
    30601 cagggtttca gtgattgggt agcctgcatt ggttgaaagc tcggtcagcg catgatgtgt
    30661 ctcttcagag atgcgtttct gcatatcacc cagattcaca tcgataagcc cggtgtatag
    30721 gtcagagtag tttacatcta cgtggatcag agaaactttc gcattgtagg ggcgagccat
    30781 agagactgct ttctctacca gaactttgct ttccggggag aggtcgaccg cgatgagaat
    30841 gtgtttataa gccatagtgt tactccttcc ataaagttgt cgatgactgg ccagctagcg
    30901 tttcttgtgc cgttacgtca cccgcgtcct gcgaactacg tgcgcggggc taacccagcc
    30961 gtaaagattc agtgttaaga ccatccttac cttatagcga cccggatgat ccgtcaatcc
    31021 gccttgctta ctcgttagat aaacaaattt tcgacatgaa tatattgata gtggttaacc
    31081 ttctggaaaa aaaacaacct gatctcctac actatctata gagccgctcg gatgttggtc
    31141 accacaaaag cggtttctcc aggacctggt gttaactgat tggcagcgta tcgggagcgg
    31201 tagccccggg gtggaactcc gcgggcaggt cgccggggag gaggtatgat aagcaccgtc
    31261 gcattatttt gggctttatg tgtcgtttgc attgttaaca tggcgcgcta tttctcatca
    31321 ctacgcgcgt tgttagtggt actgcgtaac tgcgatccat tgctctatca atatgttgat
    31381 ggagggggct tttttacctc acatggccaa cccaacaaac agatgcgtct cgtttggtat
    31441 atctatgccc aacgttatcg cgatcatcac gatgatgagt ttattcgccg ctgtgagcgg
    31501 gtgcgtcggc agtttattct gaccagcgca ttgtgtggtc tggtggtggt cagcctgatt
    31561 gcattgatga tttggcatta agcacttcag caataatgac gcataaaaaa agcgggccag
    31621 taaactgacc cgctcgtttt ggtgcgtacg attacaggaa ctgcaaggag agccagtaca
    31681 gcccgccgga aagcagtacc gcagccggaa gggtaaacac ccaggccatc agaatactgg
    31741 ttacggtttt acgctgtaag ccgccgccat ctaccaccat cgtccccgcg acagaagagg
    31801 agagtacgtg agtagtggaa accggcatcc cggtataact tgccaggccg atagacacag
    31861 ccgccgtcat ctgggcagac atcccctgag cgtaggtcat gcctttctta ccgattttct
    31921 caccgatagt cgttgccaca cgacgccagc caatcatcgt accgatacct aacgccagcg
    31981 cgaccgccat gatgatccac accggcgcat actcgatggt gctaagcatg tcggacttca
    32041 gtttcttcaa caggcgctga tcgtcagcac tcacaccagg catcttcacc actttgtcga
    32101 tagtgtcaga aacgcacagc ataatgcggc gcatctggct acgttgatca agcgacagct
    32161 tgtcgtagct ttccacatcg gtagtcaaca tgcctttcag gcggttgagc gcgttaatgg
    32221 tattcgacgg atggcaatgg aactccgcgg gttgcgttgc gccagcttcc ggagccggta
    32281 ctaactgatc agcaccggtc gcttgtttca gcagcgcagg atgctgctca aagtaagctt
    32341 cgacgttgtt gatggcatca cgggtacggg tgatttcgta gccagtggca ttcatgttca
    32401 ccacgaagcc tgctggtgcg acgccaatca ataccaacat aaccagacca atgcctttct
    32461 gaccatcgtt cgcgccatgc gaaaatgcca cgccgatagc ggaaaggatc agcgcaatac
    32521 gcgtccagaa cggcggcttt ttcttgccgt ctttcttttc acgctccgct ggggtcaggt
    32581 ggatacgggc gcgtttcttg gtgccgctcc agtagcgacg cagcaagaaa atcagaccgc
    32641 cagcaaacac caggccgaca ataggggaaa cgatcagaga accgaaaata cttaatactt
    32701 tcgggatatt gagtgcatcc accactgacg tcccggtcat caacgcattg gttaaaccaa
    32761 tcccgatgat cgcgccaatc agcgtatgag agctggatgc aggtaaacca aagtaccagg
    32821 tacccaggtt ccagataatc gccgccagca acatagagaa caccatggca aggccatgag
    32881 acgatcccat attaagcagc agatccgtcg gcagcatatg cacaatggca taggcaacac
    32941 tcagaccacc cagcaaaaca cccaaaaagt tgaataccgc cgccataacc acggcgagct
    33001 gagaacgcat cgcgcgggta tagataacgg ttgccacggc gttggctgtg tcatggaaac
    33061 cattgatggc ttcgtagaac agcacaaaag ccagtgcaag caataataac agcccggtat
    33121 gcaaatccag gccagcaaac aaatgtagca taggacgtta cgccattttg aggacatgaa
    33181 cgcggcgcat tatcagtgac tttcgcggcg cgggcaaagt gaaatataga ctttttttga
    33241 tttgcctcct gtatggattc cactcaaaaa ataattatct tatataattc aggcaaatac
    33301 ttccttttag taatattgat gctggtgcga ccactgagga atctttacaa ttcacgcccg
    33361 ttttttctta gaggagcgca acgtggaaag gtttgatgcc attattatag gcgctggtgc
    33421 ggcaggtatg ttctgttctg cgctggcagg tcaggcagga cgccgggttc tgctgatcga
    33481 taatggtaaa aaaccagggc gcaaaatcct catgtctggc ggtgggcgct gcaactttac
    33541 caacctttat gtcgaaccag gcgcttatct gagccagaat ccgcattttt gtaagtctgc
    33601 actcgcgcgt tttacccagt gggatttcat tgatctggtc aataaacacg gcatcacctg
    33661 gcacgagaaa acgttagggc aactcttctg cgatgactcc gcgcagcaga ttgtcgacat
    33721 gctggtggat gagtgcgaga agggcaatgt ggccttcaga ttgcgtagcg aagtgctgag
    33781 cgtggcgaag gatgatacag gcttcacgct tgaactgaac ggcatgactg tcggttgcga
    33841 aaagctggtc atcgcgaccg gtggactgtc aatgccgggg ctgggcgcat cgccgtttgg
    33901 ttataagatt gccgaacaat tcggcctcaa cgtgctgccg acccgtgcgg gtctggtacc
    33961 attcactctg cataaaccgt tgcttgaaga gttacaggtg ctggcgggcg tggcggtgcc
    34021 ttccgtgatt accgctgaaa acggtaccgt tttccgtgag aacttactct tcacccaccg
    34081 cggcttatct ggaccggcgg tgttgcagat ttccagctac tggcaaccgg gtgaatttgt
    34141 cagcatcaat ctgctaccgg atgtggacct cgaaaccttc ctgaatgagc agcgtaacgc
    34201 acatccgaat caaagcctga aaaacacgct ggcggttcat ctgccgaagc ggttggttga
    34261 acggttacaa caactcgggc aaatcccgga tgtctcgcta aaacagctca acgtgcgtga
    34321 ccaacagaca ctgattagca cattgactga ctggcgcgta caacccaacg gcactgaagg
    34381 ctatcgcact gccgaagtga cgctcggcgg cgtggacacc aacgaactct cttcacggac
    34441 gatggaagcg cgcaaagtgc ctgggctgta cttcatcggc gaagtgatgg acgtcaccgg
    34501 ctggctgggg ggctataact tccagtgggc gtggtcgagt gcatgggctt gtgcgcagga
    34561 tttggtggca gaataagaag caaaatgcgc tctcgatacc agagggatta acctgaataa
    34621 aaccagataa ggcgctcaac gccttatctg aagccttttt tattattttt tcgctgaacc
    34681 cgcttctaat atagaaacga tggaaaacag tgtaaaacaa actgcaccta agccaaccag
    34741 aactcgcgaa ggaataaagt aagccatatc ggtttgcgca atttcagcca ggaacgcagc
    34801 aaggaacaga caaaacagac aggtaaatac cggaatcatg ggtattctgt tggctaacga
    34861 acaggtgcgt cgccatacca gcgctaacaa ccacactttg gagaaaatac tgtagcagat
    34921 catccccagg caaatgagaa taatgccggg ggccaggcgg gcgcttgcat ctgaactgat
    34981 taatacgtag ataccaaaaa taatggtaag tgagccaagc aaaataaccc aatagcacca
    35041 cagccaatgt tctttcactg agaacgtatt ccgcgtttga tggacaatag tggcgacaag
    35101 accaataaga caagcgcaaa ttgccgttaa ccccattagt acgtgacctg caacataatg
    35161 tggtgtgata tctgcactac gtaacagtat gactgaccaa atgaaaccaa agagagtaag
    35221 cagaacggga acggcaatta agcaattacc gattgttgaa gaataggcct gcaatggcgt
    35281 accgtcactc ttactgcccg aggcgttttt ggggatcaac agaaaatgtc cggaagatgc
    35341 cgctacggtt gatacacaag cggcaatcat gccaacgccg aaaataacat gaccggcgac
    35401 aaattcatct gccatcacat tattgcttgc caacagtgac catccccaaa tcatagttgt
    35461 agcagaaccg gcataaccaa taacaggaaa taacctgttg tagaatttat tcatgctgtg
    35521 agttaactga gatataataa taaatgcggt agtgaacaat gccagacata tcgcagaaag
    35581 agaaatcaga acatgccctg caacgaaata ttcactttgc ccagaaccgg ttaatacaaa
    35641 ctcacctaat gcgatgcata ttatacccat cagtaaagga attaatttaa acaaccatcc
    35701 gatatatata ttcattttaa accccatata tattttctgc tacacttcga aaaacatatg
    35761 at
//
LOCUS       DAHHTG010000037        35253 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.037, whole genome shotgun sequence.
ACCESSION   DAHHTG010000037 DAHHTG010000000
VERSION     DAHHTG010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 35253)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35253)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35253
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.037"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            14..1279
                     /gene="uidC"
                     /locus_tag="NBI74_001810"
     CDS             14..1279
                     /gene="uidC"
                     /locus_tag="NBI74_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide uptake porin UidC"
                     /protein_id="HCD1932166.1"
                     /translation="MRKIVAMAVICLTAASGLTSAYAAQLADDEAGLRIRLKNELRRA
                     DKPSAGTGRDIYAWVQGGLLDFNSGYYSNIIGVEGGAYYVYKLGARADMSTRWYLDGD
                     KSFGFALGAVKIKPSENSLLKLGRFGTDYSYGSLPYRIPLMAGSSQRTLPTVSEGALG
                     YWALTPNIDLWGMWRSRVFLWTDSTTGIRDEGVYNSQTGKYDKHRARSFLAASWHDDT
                     SRYSLGASVQKDVSNQIQSILEKSIPLDPNYTLKGELLGFYAQLEGLSRNTSQPNETA
                     LVSGQLTWNAPWGSVFGSGGYLRHAMNGAVVDTDIGYPFSLSLDRNREGMQSWQLGVN
                     YRLTPQFTLTFAPIVTRGYESSKRDVRIEGAGILGGMNYRVSEGPLQGMNFFLAADKG
                     REKRDGSTLGDRLNYWDVKMSIQYDFMLK"
     gene            complement(1324..2832)
                     /locus_tag="NBI74_001811"
     CDS             complement(1324..2832)
                     /locus_tag="NBI74_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HCD1932167.1"
                     /translation="MNKSLVAVGVIVALGVVWTGGAWYTGKKIETHLEDMVAQANAQL
                     KLTAPESNLEVSYQNYHRGVFSSQLQLLVKPIAGKVNPWIKSGQSVIFNESVDHGPFP
                     LAQLKKLNLIPSMASIQTTLVNNEVSKPLFDMAKGETPFEINSRIGYSGDSSSDISLK
                     PLNYEQKDEKVAFSGGEFQLNADRDGKAISLSGEAQSGRIDAVNEYNQKVQLTFNNLK
                     TDGSSTLASFGERVGNQKLSLEKMTISVEGKELALLEGMEISGKSDLVNDGKTINSQL
                     DYSLNSLKVQNQDLGSGKLTLKVGQIDGEAWHQFSQQYNAQTQALLAQPEIANNPELY
                     QEKVTEAFFSALPLMLKGDPVITIAPLSWKNSQGESALNLSLFLKDPATTKEAPQTLA
                     QEVDRSVKSLDAKLTIPVDMATELMTQVAKLEGYQEDQAKKLAKQQVEGASAMGQMFR
                     LTTLQDNTITTSLQYANGQITLNGQKMPLEDFVGMFAMPTLNVPAVPAIPQQ"
     gene            complement(2933..4108)
                     /gene="manA"
                     /locus_tag="NBI74_001812"
     CDS             complement(2933..4108)
                     /gene="manA"
                     /locus_tag="NBI74_001812"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416130.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase"
                     /protein_id="HCD1932168.1"
                     /translation="MQKLINSVQNYAWGSKTALTELYGMENPSSQPMAELWMGAHPKS
                     SSRVHDAAGDIVSLRDVIESDKATLLGDAVAKRFGELPFLFKVLCAAQPLSIQVHPNK
                     RNSEIGFAKENAAGIPMDAAERNYKDPNHKPELVFALTPFLAMNAFREFSEIVSLLQP
                     VAGAHPAIAHFLQQPDAERLSELFASLLNMQGEEKSRALAILKSALDSQQGEPWQTIR
                     LISEFYPEDSGLFSPLLLNVVKLNPGEAMFLFAETPHAYLQGVALEVMANSDNVLRAG
                     LTPKYIDIPELVANVKFEAKPANKLLTQPVKQGAELDFPIPVDDFAFSLHDLSDKETT
                     ISQQSAAILFCVEGDATLCKGSQQLQLKPGESAFIAANESPVTVKGHGRLARVYNKL"
     gene            4307..5953
                     /gene="fumB"
                     /locus_tag="NBI74_001813"
     CDS             4307..5953
                     /gene="fumB"
                     /locus_tag="NBI74_001813"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000066594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HCD1932169.1"
                     /translation="MSNKPFHYQAPFPLKKDDTEYYLLTSEHVSVSEFEGQEILKVAP
                     EALTLLARQAFHDASFMLRPAHQQQVADILCDPEASENDKYVALQFLRNSDIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGGDEAALARGVYNTYIEDNLRYSQNAPLDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCIAKGGGSANKTYLYQETKALLTPGKLKNYLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAKYYDELPTEGNEHGQAFRDVELEKEL
                     LIEAQNLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINRQGIW
                     IEKLEHNPGKYIPEELRKAGEGEAVRVDLNRPMKEILAQLSQYPVSTRLSLNGTIIVG
                     RDIAHAKLKERMDNGEGLPQYIKDHPIYYAGPAKTPEGYASGSLGPTTAGRMDSYVDQ
                     LQAQGGSMIMLAKGNRSQQVTDACKKHGGFYLGSIGGPAAVLAQGSIKSLECVEYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIQLTQCTRCVK"
     gene            6096..7499
                     /gene="fumC"
                     /locus_tag="NBI74_001814"
     CDS             6096..7499
                     /gene="fumC"
                     /locus_tag="NBI74_001814"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001099101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HCD1932170.1"
                     /translation="MNTVRSEKDSMGAIDVPADKLWGAQTQRSLEHFRISTEKMPTSL
                     IHALALTKRAAAKVNEDLGLLSEEKASAIRQAADEVLAGQHDDEFPLAIWQTGSGTQS
                     NMNMNEVLANRASELLGGVRGMERKVHPNDDVNKSQSSNDVFPTAMHVAALLALRKQL
                     IPQLKTLTQTLSEKSRAFADIVKIGRTHLQDATPLTLGQEISGWVAMLEHNLKHIEYS
                     LPHVAELALGGTAVGTGLNTHPEYARRVADELAVITCAPFVTAPNKFEALATCDALVQ
                     AHGALKGLAASLMKIANDVRWLASGPRCGIGEISIPENEPGSSIMPGKVNPTQCEALT
                     MLCCQVMGNDVAINMGGASGNFELNVFRPMVIHNFLQSVRLLADGMESFNKHCAVGIE
                     PNRERINQLLNESLMLVTALNTHIGYDKAAEIAKKAHKEGLTLKAAALALGYLSEAEF
                     DRWVRPEQMVGSMKAGG"
     gene            complement(7496..8425)
                     /gene="tus"
                     /locus_tag="NBI74_001815"
     CDS             complement(7496..8425)
                     /gene="tus"
                     /locus_tag="NBI74_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication terminus site-binding protein"
                     /protein_id="HCD1932171.1"
                     /translation="MARYDLVDRLNTTFRQMEQELAAFAAHLEQHKLLVARVFSLPEV
                     KKEDEHNPLNRIEVKQHLGNDAQSLALRHFRHLFIQQQSENRSSKAAVRLPGVLCYQV
                     DNFSQAALVSHIQHINKLKTTFEHIVTVESELPSVARFEWVHRHFPGLITLNAYRTLT
                     VLHDPATLRFGWANKHIIKNLHRDEVLAQLEKSLKSPRSVAPWTREEWQRKLEREYQD
                     IAALPQNAKLKIKRPVKVQPIARVWYKGDQKQVQHACPTPLIALINRDNGAGVPDVGE
                     LLNYDADNVQHRYKPQAQPLRLIIPRLHLYVAD"
     gene            complement(8501..9802)
                     /gene="rstB"
                     /locus_tag="NBI74_001816"
     CDS             complement(8501..9802)
                     /gene="rstB"
                     /locus_tag="NBI74_001816"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RstB"
                     /protein_id="HCD1932172.1"
                     /translation="MKKLFIQFYLLLFVCFLVMSLLVGLVYKFTAERAGKQSLDDLMN
                     SSLYLMRSELREIPPHDWGKTLKEMDLNLSFDLRVEPLSKYHLDDISMHRLRGGEIVA
                     LDDQYTFLQRIPRSHYVLAVGPVPYLYYLHQMRLLDIALIAFIAISLAFPVFIWMRPH
                     WQDMLKLEAAAQRFGDGHLSERIHFDEGSSFERLGVAFNQMADNINALIASKKQLIDG
                     IAHELRTPLVRLRYRLEMSDNLSAAESQALNRDISQLEALIEELLTYARLDRPQNELH
                     LSEPDLPLWLSTHLADIQAVTPDKTVRIKTLAQGHYAALDMRLMERVLDNLLNNALRY
                     CHSTVETSLLLSGNRATLIVEDDGPGIAPENREHIFEPFVRLDPSRDRSTGGCGLGLA
                     IVHSIALAMGGTVNCDTSELGGARFSFSWPLWHNIPQFTSA"
     gene            complement(9806..10525)
                     /gene="rstA"
                     /locus_tag="NBI74_001817"
     CDS             complement(9806..10525)
                     /gene="rstA"
                     /locus_tag="NBI74_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RstA"
                     /protein_id="HCD1932173.1"
                     /translation="MNTIVFVEDDAEVGALIAAYLAKHDMQVTVEPRGDRAEETILRE
                     NPDLVLLDIMLPGKDGMTICRDLRAKWSGPIVLLTSLDSDMNHILALEMGACDYILKT
                     TPPAVLLARLRLHLRQNEQATVTKGIQETSLTPYKALHFGTLTIDPINRVVSLANTEI
                     SLSTADFELLWELATHAGQIMDRDALLKNLRGVSYDGLDRSVDVAISRLRKKLLDNAA
                     EPYRIKTVRNKGYLFAPHAWE"
     gene            10654..10989
                     /locus_tag="NBI74_001818"
     CDS             10654..10989
                     /locus_tag="NBI74_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlpM family protein"
                     /protein_id="HCD1932174.1"
                     /translation="MGLVIKAALGALVVLLIGVLAKTKNYYIAGLIPLFPTFALIAHY
                     IVASERGIEALRATIIFSMWSIIPYFVYLASLWYFTGMMRLPAAFVGSVACWGISAWV
                     LIICWIKLH"
     gene            complement(10986..11708)
                     /gene="folM"
                     /locus_tag="NBI74_001819"
     CDS             complement(10986..11708)
                     /gene="folM"
                     /locus_tag="NBI74_001819"
                     /EC_number="1.5.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydromonapterin reductase"
                     /protein_id="HCD1932175.1"
                     /translation="MGKTQSLPILITGGGRRIGLALAWHFINQKQPVIVSYRTHYPAI
                     DGLIKAGAQCIQADFSTNDGVMAFADEVLKSTHGLRAILHNASAWMAEKPGAPLTDVL
                     ACMMQIHVNTPYLLNHALERLLRGHGHAASDIIHFTDYVVERGSDKHIAYAASKAALD
                     NMTRSFARKLAPEVKVNSIAPSLILFNEHDDAEYRQQALNKSLMKTAPGEKEVIDLVD
                     YLLTSCFVTGRSLPLDGGRHLR"
     gene            complement(11745..13127)
                     /locus_tag="NBI74_001820"
     CDS             complement(11745..13127)
                     /locus_tag="NBI74_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCD1932176.1"
                     /translation="MEKKLGLSALTALVLSSMLGAGVFSLPQNMAAVASPAALLIGWG
                     ITGAGILLLAFAMLILTRIRPELDGGIFTYAREGFGELIGFCSAWGYWLCAVIANVSY
                     LVIVFSALSFFTDTPELRLFGDGNTWQSIVGASALLWIVHFLILRGVQTAASINLVAT
                     LAKLLPLGLFVVLAMMMFKLDTFKLDFTGLALGVPVWEQVKNTMLITLWVFIGVEGAV
                     VVSARARNKRDVGKATLLAVLSALGVYLLVTLLSLGVVARPELAEIRNPSMAGLMVEM
                     MGPWGEIIIAAGLIVSVCGAYLSWTIMAAEVPFLAATHKAFPRIFARQNAQAAPSASL
                     WLTNICVQICLVLIWLTGSDYNTLLTIASEMILVPYFLVGAFLLKIATRPLHKAVGVG
                     ACIYGLWLLYASGPMHLLLSVVLYAPGLLVFLYARKTHTHDNVLNRQEMVLIGMLLIA
                     SVPATWMLVG"
     gene            complement(13313..14257)
                     /locus_tag="NBI74_001821"
     CDS             complement(13313..14257)
                     /locus_tag="NBI74_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HCD1932177.1"
                     /translation="MKLKNTLLASALLSATAFSVNAATELTPEQAAAVKPFDRVVVTG
                     RFNAIGEAVKAVSRRADKEGAASFYVVDTSDFGNSGNWRVVADLYKADAEKAEETSNR
                     VINGVVELPKDQAVLIEPFDTVTVQGFYRSQPEVNDAITKAAKAKGAYSFYIVRQIDA
                     NQGGNQRITAFIYKKDAKKRIVQSPDVIPADSEAGRAALAAGGEAAKKVEIPGVATTA
                     SPSSEVGRFFETQSSKGGRYTVTLPDGTKVEELNKATAAMMVPFDSIKFSGNYGNMTE
                     VSYQVAKRAAKKGAKYYHITRQWQERGNNLTVSADLYK"
     gene            14781..16313
                     /gene="pntA"
                     /locus_tag="NBI74_001822"
     CDS             14781..16313
                     /gene="pntA"
                     /locus_tag="NBI74_001822"
                     /EC_number="1.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HCD1932178.1"
                     /translation="MRIGIPRERLTNETRVAATPKTVEQLLKLGFTVAVESGAGQLAS
                     FDDKAFVQAGAEIVEGNSVWQSEIILKVNAPLDDEIALLNPGTTLVSFIWPAQNPELM
                     QKLAERNVTVMAMDSVPRISRAQSLDALSSMANIAGYRAIVEAAHEFGRFFTGQITAA
                     GKVPPAKVMVIGAGVAGLAAIGAANSLGAIVRAFDTRPEVKEQVQSMGAEFLELDFKE
                     EAGSGDGYAKVMSDAFIKAEMELFAAQAKEVDIIVTTALIPGKPAPKLITREMVDSMK
                     AGSVIVDLAAQNGGNCEYTVPGEIFTTENGVKVIGYTDLPGRLPTQSSQLYGTNLVNL
                     LKLLCKEKDGNITVDFDDVVIRGVTVIRAGEITWPAPPIQVSAQPQTAQKAAPEVKTE
                     EKCACSPWRKYALMALAIILFGWMASVAPKEFLGHFTVFALACVVGYYVVWNVSHALH
                     TPLMSVTNAISGIIVVGALLQIGQGGWVSFLSFIAVLIASINIFGGFTVTQRMLKMFR
                     KN"
     gene            16324..17712
                     /gene="pntB"
                     /locus_tag="NBI74_001823"
     CDS             16324..17712
                     /gene="pntB"
                     /locus_tag="NBI74_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000014033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit beta"
                     /protein_id="HCD1932179.1"
                     /translation="MSGGLVTAAYIVAAILFIFSLAGLSKHETSRQGNNFGIAGMAIA
                     LIATIFGPDTGNVGWILLAMVIGGAIGIRLAKKVEMTEMPELVAILHSFVGLAAVLVG
                     FNSYLHHDAGMAPILVNIHLTEVFLGIFIGAVTFTGSVVAFGKLCGKISSKPLMLPNR
                     HKMNLAALIVSFLLLIVFVRTDSVGLQVLALLIMTAIALVFGWHLVASIGGADMPVVV
                     SMLNSYSGWAAAAAGFMLSNDLLIVTGALVGSSGAILSYIMCKAMNRSFISVIAGGFG
                     TDGSSTGDDQEVGEHREITAEETAELLKNSHSVIITPGYGMAVAQAQYPVAEITEKLR
                     ARGINVRFGIHPVAGRLPGHMNVLLAEAKVPYDIVLEMDEINDDFADTDTVLVIGAND
                     TVNPAAQDDPKSPIAGMPVLEVWKAQNVIVFKRSMNTGYAGVQNPLFFKENTHMLFGD
                     AKASVDAILKAL"
     gene            complement(17737..18771)
                     /gene="tqsA"
                     /locus_tag="NBI74_001824"
     CDS             complement(17737..18771)
                     /gene="tqsA"
                     /locus_tag="NBI74_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2 transporter TqsA"
                     /protein_id="HCD1932180.1"
                     /translation="MAKPIITLNGLKIVIMLGMLVIILCGIRFAAEIIVPFILALFIA
                     VILNPLVQHMVRWRVPRVLAVSILMTIIVMAMVLLLAYLGSALNELTRTLPQYRNSIM
                     TPLQAIEPLLQRVGIDVSVDQLAHYIDPNAAMTLLTNLLTQLSNAMSSIFLLLLTVLF
                     MLLEVPQLPGKFQQMMARPVEGMAAIQRAIDSVSHYLVLKTAISIITGLVAWAMLAAL
                     DVRFAFVWGLLAFALNYIPNIGSVLAAIPPIAQVLVFNGFYEALLVLAGYLLINLVFG
                     NILEPRIMGRGLGLSTLVVFLSLIFWGWLLGPVGMLLSVPLTIIVKIALEQTAGGQSI
                     AVLLSDLNKE"
     gene            19183..19548
                     /gene="mdtJ"
                     /locus_tag="NBI74_001825"
     CDS             19183..19548
                     /gene="mdtJ"
                     /locus_tag="NBI74_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtJ"
                     /protein_id="HCD1932181.1"
                     /translation="MYIYWILLGLAIATEITGTLSMKWASVSEGNGGFILMLVMISLS
                     YIFLSFAVKKIALGVAYALWEGIGILFITLFSVLLFDESLSLMKIAGLTTLVAGIVLI
                     KSGTRKARKPELEVNHGAV"
     gene            19535..19864
                     /gene="mdtI"
                     /locus_tag="NBI74_001826"
     CDS             19535..19864
                     /gene="mdtI"
                     /locus_tag="NBI74_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtI"
                     /protein_id="HCD1932182.1"
                     /translation="MAQFEWVHAAWLALAIVLEIVANVFLKFSDGFRRKIFGLLSLAA
                     VLAAFSALSQAVKGIDLSVAYALWGGFGIAATLAAGWILFGQRLNRKGWIGLVLLLAG
                     MIMVKLA"
     gene            complement(19903..20724)
                     /locus_tag="NBI74_001827"
     CDS             complement(19903..20724)
                     /locus_tag="NBI74_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HCD1932183.1"
                     /translation="MRTTIAVVLGAISLTSAFVFADKPDVAKSANDEVSTLFFGHDDR
                     VPVNDTTQSPWDAVGQLETASGNLCTATLIAPNLALTAGHCLLTPPKGKADKAVALRF
                     VSNKGLWRYEIHDIEGRVDPTLGKRLKADGDGWIVPPAAAPWDFGLIVLRNPPSGITP
                     LPLFEGDKAALTAALKAAGRKVTQAGYPEDHLDTLYSHQNCEVTGWAQTSVMSHQCDT
                     LPGDSGSPLMLHTDDGWQLIGVQSSAPAAKDRWRADNRAISVTGFRDKLDQLSQK"
     gene            complement(20824..20907)
                     /locus_tag="NBI74_001828"
     CDS             complement(20824..20907)
                     /locus_tag="NBI74_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1932184.1"
                     /translation="MVGRYRFEFILIILILCALIAARFYLS"
     gene            complement(21000..21335)
                     /locus_tag="NBI74_001829"
     CDS             complement(21000..21335)
                     /locus_tag="NBI74_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid shock protein"
                     /protein_id="HCD1932185.1"
                     /translation="MKKQIEGMTMKKVLALVVAAAMGLSSAAFAAETATTPAPTATTT
                     KAAPAKTTHHKKQHKAAPAQKAQAAKKHHKNAKAEQKAPEQKAQAAKKHAKKHSHQQP
                     AKPAAQPAA"
     gene            complement(21732..22985)
                     /locus_tag="NBI74_001830"
     CDS             complement(21732..22985)
                     /locus_tag="NBI74_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCD1932186.1"
                     /translation="MSRTTTVDGAPASDTDKQSISQPNHFIKRGTPQFMRVTLALFSA
                     GLATFALLYCVQPILPVLSQEFGLTPATSSISLSISTAMLAIGLLFTGPLSDAIGRKP
                     VMVTALLLASICTLLSTMMTSWHGILIMRALIGLSLSGVAAVGMTYLSEEIHPSFVAF
                     SMGLYISGNSIGGMSGRLISGVFTDFFNWRIALAAIGCFALASALMFWKILPESRHFR
                     PTSLRPKTLFINFRLHWRDRGLPLLFAEGFLLMGSFVTLFNYIGYRLMLSPWHVSQAV
                     VGLLSLAYLTGTWSSPKAGTMTARYGRGPVMLFSTGVMLFGLLMTLFSSLWLIFAGML
                     LFSAGFFAAHSVASSWIGPRAKRAKGQASSLYLFSYYLGSSIAGTLGGVFWHNYGWNG
                     VGAFIALMLVIALLVGTRLHHRLHA"
     gene            23092..23985
                     /locus_tag="NBI74_001831"
     CDS             23092..23985
                     /locus_tag="NBI74_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCD1932187.1"
                     /translation="MNIELRHLRYFVAVAEELHFGRAAARLNISQPPLSQQIQALEQQ
                     IGARLLARTNRSVLLTEAGKQFLVDSRQILSMVDDAAARAERLHQGEAGELRIGFTSS
                     APFIRAVSDTLSLFRRDYPDVHLQTREMNTREQIAPLIEGTLDMGLLRNTALPETLEH
                     AVIVHEPLMAMIPHDHPLANNPSVTLAELAKEPFVFFDPHVGTGLYDDILGLMRRYNL
                     TPVITQEVGEAMTIIGLVSAGLGVSILPASFKRVQLNEMRWVPIAEEDAVSEMWLVWP
                     KHHEQSPAARNFRIHLLNALR"
     gene            24120..25340
                     /gene="mlc"
                     /locus_tag="NBI74_001832"
                     /gene_synonym="dgsA"
     CDS             24120..25340
                     /gene="mlc"
                     /locus_tag="NBI74_001832"
                     /gene_synonym="dgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor Mlc"
                     /protein_id="HCD1932188.1"
                     /translation="MVAENQPGHIDQIKQTNAGAVYRLIDQLGPVSRIDLSRLAQLAP
                     ASITKIVREMLEAHLVQELEIKEAGNRGRPAVGLVVETEAWHYLSLRISRGEIFLALR
                     DLSSKLVVEESQELALKDDSPLLDRIISHIDQFFIRHQKKLERLTSIAITLPGIIDTE
                     NGIVHRMPFYEDVKEMPLGEALEQHTGVPVYIQHDISAWTMAEALFGASRGARDVIQV
                     VIDHNVGAGVITDGHLLHAGSSSLVEIGHTQVDPYGKRCYCGNHGCLETIASVDSILE
                     LAQLRLNQSMSSMLHGQPLTVDSLCQAALRGDLLAKDIITGVGAHVGRILAIMVNLFN
                     PQKILIGSPLSKAADILFPVISDSIRQQALPAYSQHISVESTQFSNQGTMAGAALVKD
                     AMYNGSLLIRLLQG"
     gene            25465..26160
                     /gene="bioD"
                     /locus_tag="NBI74_001833"
     CDS             25465..26160
                     /gene="bioD"
                     /locus_tag="NBI74_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent dethiobiotin synthetase BioD"
                     /protein_id="HCD1932189.1"
                     /translation="MLKRFFITGTDTSVGKTVVSRALLQALASQGKTVAGYKPVAKGS
                     KETPEGLRNKDALVLQSVSTIELPYEAVNPIALSEEESSVAHSCPINYTLISNGLANL
                     TDKVDHVVVEGTGGWRSLMNDLRPLSEWVVQEQLPVLMVVGIQEGCINHALLTAQAIA
                     NDGLPLIGWVANRINPGLAHYAEIIDVLGKKLPAPLIGELPYLPRAEQRELGQYIRLA
                     MLRSVLAVDRVTV"
     gene            complement(26113..27405)
                     /gene="clcB"
                     /locus_tag="NBI74_001834"
     CDS             complement(26113..27405)
                     /gene="clcB"
                     /locus_tag="NBI74_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated ClC-type chloride channel ClcB"
                     /protein_id="HCD1932190.1"
                     /translation="MHHLHIYPDLRTMFRRLLIATIVGILAAFAVAGFRHAMLLLEWL
                     FLNNDSGSLVNAATNLSPWRRLLTPALGGLAAGLLLMGWQKFTQQRPHAPTDYMEALQ
                     TDGQFDYAASLVKSLASLLVVTSGSAIGREGAMILLAALAASCFAQRFTPRQEWKLWI
                     ACGAAAGMAAAYRAPLAGSLFIAEVLFGTMMLASLGPVIISAVVALLVSNLINHSDAL
                     LYSVQLSVTVQARDYALIISTGVLAGLCGPLLLTLMNACHRGFVSLKLAPPWQLALGG
                     LIVGLLSLFTPAVWGNGYSTVQSFLTAPPLLMIIAGIFLCKLCAVLASSGSGAPGGVF
                     TPTLFIGLAIGMLYGRSLGLWLPDGEEITLLLGLTGMATLLAATTHAPTMSTLMICEM
                     TGEYQLLPGLLIACVIASVISRTLHRDSIYRQHTAKHS"
     gene            complement(27566..28180)
                     /gene="dmsD"
                     /locus_tag="NBI74_001835"
     CDS             complement(27566..28180)
                     /gene="dmsD"
                     /locus_tag="NBI74_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tat proofreading chaperone DmsD"
                     /protein_id="HCD1932191.1"
                     /translation="MTHFSQQDNFSVAARVLGALFYYAPESAEAAPLVAVLTNDGWET
                     QWPLPEASLAPLVTAFQTQCEETHAQAWQRLFVGPWALPSPPWGSVWLDRESVLFGDS
                     TLALRQWMREKGIQFEMKQNEPEDNFGSLLLMAAWLAENGRQTECEELLAWHLFPWST
                     RFLDVFIEKAEHPFYRALGELARLTLAQWQSQLLIPVAVKPLFR"
     gene            complement(28223..29077)
                     /locus_tag="NBI74_001836"
     CDS             complement(28223..29077)
                     /locus_tag="NBI74_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit"
                     /protein_id="HCD1932192.1"
                     /translation="MGNGWHEWPLVIFTVLGQCVVGALIVSGIGWFAAKNDADRQHII
                     RGMFFLWLLMGIGFIASVMHLGSPLRAFNSLNRIGASGLSNEIAAGSIFFAVGGLWWL
                     VAVIGKMPQALGKLWLLVSMALGIIFVWMMTCVYQIDTVPTWHNGYTTLAFFLTVLLS
                     GPILAATILRAARVTFNTTPFAIISILALIACAGLIVLQGLSLASIHSSVQQASALVP
                     DYASLQVWRVVLLCAGLGCWLCPLIRRREPHVAGLVLGLILILGGEMIGRVLFYGLHM
                     TVGMAIAG"
     gene            complement(29079..29696)
                     /gene="dmsB"
                     /locus_tag="NBI74_001837"
     CDS             complement(29079..29696)
                     /gene="dmsB"
                     /locus_tag="NBI74_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit B"
                     /protein_id="HCD1932193.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDFKDLGPEVSFRRIYEYAG
                     GDWQEDNGVWHQNVFAYYLSISCNHCDDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNAEKGHMTKCDGCYSRVAEGKQPICVESCPLRALEFGPIEELRQ
                     KHGTLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPEEV"
     gene            complement(29707..31276)
                     /locus_tag="NBI74_001838"
                     /pseudo
     CDS             complement(29707..31276)
                     /locus_tag="NBI74_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416105.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
     gene            complement(31337..31753)
                     /locus_tag="NBI74_001839"
                     /pseudo
     CDS             complement(31337..31753)
                     /locus_tag="NBI74_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310320.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase subunit A"
     gene            31795..33164
                     /locus_tag="NBI74_001840"
     CDS             join(31795..32275,32275..33164)
                     /locus_tag="NBI74_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611947.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS150 family transposase"
                     /protein_id="HCD1932194.1"
                     /translation="MSKPKYPFEKRLEVVNHYFTTDDGYRIISARFGVPRTQVRTWVA
                     LYEKHGEKGLIPKPKGVSADPELRIKVVKAVIEQHMSLNQAAAHFMLAGSGSVARWLK
                     VYEERGEAGLRALKIGTKRNIAISVDPEKAASALELSKDRRIEDLERQVRFLETRLMY
                     PKKAESLSSSHEKVKVLNELRQFYPLDELLRAAEIPRSTFYYHLKALSKPDKYADVKK
                     RISEIYHENRGRYGYRRVTLSLHREGKQINHKAVQRLMGTLSLKAAIKVKRYRSYRGE
                     VGQTAPNVLQRDFKATRPNEKWVTDVTEFAVNGRKLYLSPVIDLFNNEVISYSLSERP
                     VMNMVENMLDQAFKKLNPHEHPVLHSDQGWQYRMRRYQNILKEHGIKQSMSRKGNCLD
                     NAVVECFFGTLKSECFYLDEFSNISELKDAVTEYIEYYNSRRISLKLKGLTPIEYRNQ
                     TYMPRV"
     gene            complement(33191..35209)
                     /locus_tag="NBI74_001841"
                     /pseudo
     CDS             complement(33191..35209)
                     /locus_tag="NBI74_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310320.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
BASE COUNT         8938 a         8581 c         9047 g         8687 t
ORIGIN      
        1 caaaggtgca actatgagaa aaatagtggc catggccgtt atttgcctga cggctgcctc
       61 tggccttacc tctgcttacg cggcacaact ggctgacgat gaagcgggac tacgcatcag
      121 actgaaaaac gaattacgca gggctgataa gcccagtgct ggcacgggaa gagatattta
      181 cgcatgggta cagggaggat tgctcgattt caatagtggt tattattcca atattattgg
      241 cgttgaaggc ggggcgtatt atgtttataa attaggtgct cgtgctgata tgagtacccg
      301 gtggtatctt gatggtgata aaagttttgg ctttgccctg ggggcagtaa aaataaaacc
      361 cagtgaaaat agcctgctaa aattaggtcg cttcgggacg gattatagtt atggtagctt
      421 accttatcgt attccgttaa tggctggcag ttcgcaacgt acattaccga ctgtttctga
      481 aggagcatta ggttattggg ctttaacgcc aaatattgat ctgtggggaa tgtggcgttc
      541 acgagtattt ttatggaccg attcaacaac cggtattcgt gatgaaggtg tttataacag
      601 ccagacggga aaatacgata aacatcgcgc acgttctttt ttagccgcca gttggcatga
      661 tgataccagt cgctattctc tgggggcatc ggtacagaaa gatgtttcca atcagataca
      721 aagtattctc gagaaaagca taccgcttga cccgaattat acgttgaaag gggagttgct
      781 cggcttttac gcgcaacttg aaggtttaag tcgtaatacc agccagccca atgaaacggc
      841 gttggttagt ggacaattga cctggaatgc gccgtgggga agcgtatttg gcagtggtgg
      901 ttatttgcgc catgcaatga atggtgccgt ggtggatacc gacattggct atcccttttc
      961 attaagtctt gatcgtaacc gtgaaggaat gcagtcctgg caattgggcg tcaactatcg
     1021 tttaactccg caatttacgc tgacatttgc accgattgtg acccgcggct atgaatccag
     1081 taaacgagat gtgcggattg aaggagcggg tatcttaggg ggtatgaact atcgggtcag
     1141 cgaagggccg ttacaaggga tgaatttctt tcttgctgcc gataaaggac gggaaaagcg
     1201 cgatggcagt acgctgggcg atcgcctgaa ttactgggat gtgaaaatga gtattcagta
     1261 tgactttatg ctgaagtaag aaaaaacgcc ggagagaaaa atctccggcg ttttggctgt
     1321 gaattactgc tgcggaatag cgggtacagc cggaacgttt aatgtcggca tcgcaaacat
     1381 acccacgaaa tcttccagtg gcattttctg cccgtttaac gttatctgac cgttagcata
     1441 ttgcaggctg gtggtgatgg tattgtcctg caaggtggtc agacggaaca tctgccccat
     1501 tgctgatgca ccttcaactt gctgtttcgc cagttttttc gcttgatctt cctgataacc
     1561 ttccagcttc gctacctgag tcatcaactc agttgccata tccaccggaa tggtcagttt
     1621 cgcatccaga gatttaaccg agcggtccac ttcctgcgcc agcgtttgcg gcgcttcttt
     1681 agtcgttgcc ggatctttca ggaacagcga cagattcagc gcactttcac cctgactgtt
     1741 tttccagctt agcggcgcga tagtaatcac cggatcgcct ttcagcatca gcggcagagc
     1801 gctaaagaag gcttccgtca ctttctcctg ataaagttcg ggattgttgg caatttctgg
     1861 ctgtgccagc agcgcctgag tttgcgcgtt atattgctgg ctaaactgat gccaggcttc
     1921 gccatcaatt tggccgactt ttaaagtcag cttgccactg cccagatcct gattctgtac
     1981 cttcaggctg tttagcgagt aatccagttg gctattgatc gttttaccgt cattgaccag
     2041 atccgattta ccgctgatct ccatgccttc cagcagtgcc agttctttgc cctccactga
     2101 aatggtcatt ttttccagcg acagtttttg gtttcctacg cgctcaccaa aacttgccag
     2161 cgtgctggaa ccgtcggttt tcagattatt aaaggttaac tgcactttct ggttgtattc
     2221 gttaaccgcg tctatccgac cactttgcgc ctccccggaa agggagatag ctttgccgtc
     2281 tctgtctgca tttaactgga actcgccgcc gctaaaggcg actttttcat ccttttgctc
     2341 gtaattcagt ggcttgagcg aaatgtcgga actggaatct ccgctgtaac caatgcggga
     2401 gttaatctca aaaggcgttt caccttttgc catatcaaac agtggtttgc ttacttcgtt
     2461 attaaccagc gtggtttgaa ttgatgccat cgacgggatc agattcagtt ttttaagctg
     2521 ggcaagcggg aagggaccat gatcaaccga ttcgttgaag atgacgctct gaccgctttt
     2581 aatccacgga tttacttttc cggcaatggg tttcaccaac agttgcaact ggctgctgaa
     2641 tacgccgcga tgatagtttt gataactgac ttccaggttg gattcaggag ctgtcagttt
     2701 gagttgcgcg ttcgcctgcg cgaccatgtc ttcgagatgg gtttcaatct tcttgcctgt
     2761 ataccatgcg ccgcctgtcc agactacgcc tagcgcaaca atgacgccta ccgctaccag
     2821 cgatttattc ataatgatta tccataaaat gaaatcaggc ggactggccg cctgaaggtg
     2881 ttataagcct ttaataagct tagcaagaga tgttaatttt ttcagtaagc tcttacagct
     2941 tgttgtaaac acgcgctaaa cggccgtggc ctttgacagt caccggtgat tcgttggcgg
     3001 caataaacgc tgattcaccc ggtttaagct gtaactgctg agaaccttta cacagcgttg
     3061 catcgccttc gacgcagaac aaaatggcgg cactctgctg gctgatggtg gtttctttat
     3121 cactaaggtc atgcaacgag aaggcaaaat catccacagg aatcgggaag tccagttctg
     3181 caccttgttt caccggctgg gtcaacaact tgttagccgg tttggcttcg aacttcacgt
     3241 tggcaaccag ttccggaata tcaatgtatt taggcgtcag acctgcacgc agcacgttat
     3301 cggagttcgc catcacttcc agcgccacgc cttgcaggta agcgtgcggt gtttcagcga
     3361 acaggaacat cgcttcgcca ggattcaatt tcaccacatt cagcaacagc ggggagaaca
     3421 ggccgctgtc ttccgggtaa aattcagaaa ttaaacgaat cgtttgccat ggttcaccct
     3481 gctggctgtc gagggccgat tttaaaatcg ccagcgcgcg ggatttttct tcaccctgca
     3541 tattcaacag gctggcgaac agttcgctta aacgttcggc atcaggctgt tgtaaaaagt
     3601 gagcaatcgc cggatgtgca cctgcgaccg gctggagcag ggagacaatc tcggaaaact
     3661 cacgaaacgc gttcatcgcg aggaaagggg tcagggcgaa aaccagctca ggcttgtgat
     3721 taggatcttt atagttacgc tcagcggcat ccatcggaat acctgcagca ttttctttgg
     3781 caaaaccgat ttcagaattg cgtttgtttg gatgaacctg aatggagagc ggctgggctg
     3841 cgcataatac tttgaacagg aaaggcaact cgccaaagcg tttggcaacg gcatctccca
     3901 aaagagtcgc tttatcactc tcaatcacat cacgcagtga aacgatatct cctgcggcat
     3961 cgtgcacacg tgaactgctc tttggatgcg cgcccatcca caactcggcc atcggctggc
     4021 tggacggatt ttccatacca taaagttcag tcaacgccgt tttgctgccc caggcatagt
     4081 tttgcactga gttaatgagt ttttgcatga tcaatccctg ttttaatgtg gaaattaatc
     4141 ccgctattaa agcaagaatc ctacgggaag taacctggag ccgcaaaaag tcgtactagt
     4201 ctcagttttt gttaaaaaag tgtgtaggat attgttactc gcttttaaca gggcaacgga
     4261 acacccgccc agagcataac caaaccaggc agtaagtgag agaacaatgt caaacaaacc
     4321 ctttcattat caggctcctt ttccactcaa aaaagatgat actgagtatt acctgctaac
     4381 cagcgaacac gttagcgtat ctgaatttga agggcaggag attttgaaag tcgcacccga
     4441 agcgttaact ctgttggcgc gtcaggcgtt tcatgatgcg tcatttatgc tgcgtccggc
     4501 tcaccaacaa caggtggccg acattctgtg tgacccggag gccagcgaaa atgataaata
     4561 tgtggcgctg caattcctgc gtaactccga catcgcggca aaaggcgttc tgccaacctg
     4621 tcaggatacc ggcaccgcga ttattgttgg taaaaaaggg cagcgtgtat ggaccggtgg
     4681 cggtgatgaa gcagcgctgg cgcgcggtgt ttataacact tatatcgagg ataatctgcg
     4741 ctactcgcaa aacgcgccgc tggatatgta taaagaggtg aataccggca ccaacttgcc
     4801 agcgcagatc gatctttatg ccgttgatgg cgacgagtac aaattcctct gtatcgccaa
     4861 aggtggcggt tcggcaaaca agacgtatct ctatcaggaa accaaagcgt tactgacgcc
     4921 gggaaaactg aaaaattacc tggttgagaa gatgcgcacg ctgggtacgg cggcctgtcc
     4981 tccgtatcat attgcgttcg ttattggtgg aacttctgca gaaacgaatc ttaaaacggt
     5041 gaaactggct tccgctaaat actatgatga actgccaacg gaagggaatg agcacggtca
     5101 ggcgttccgc gatgtggaac tggaaaaaga attgctgatc gaagcgcaaa atcttggtct
     5161 gggtgcgcag tttggtggta aatacttcgc tcatgacatc cgcgtgattc gcctgccacg
     5221 tcacggcgca tcctgcccgg tcggtatggg cgtctcctgt tctgctgacc gtaatatcaa
     5281 agcgaagatc aaccgtcagg ggatctggat cgaaaaactg gaacataatc caggcaaata
     5341 tatcccggaa gagctgcgca aagcaggaga aggcgaagcg gtgcgcgttg acctcaaccg
     5401 tccgatgaaa gagatcctcg cacagttgtc gcagtatccc gtttctacac gcttatcgct
     5461 taacggcacg attatcgtcg gtcgtgatat tgctcacgcc aaactgaaag agcggatgga
     5521 taacggtgaa gggctgccgc agtatattaa agatcatccg atttactacg cgggtccggc
     5581 caaaacgccg gaaggttatg cctccggttc tcttggccca acgaccgccg gacggatgga
     5641 ttcttatgtc gatcaactgc aagcgcaggg cggaagtatg atcatgctgg cgaaaggcaa
     5701 ccgcagccag caggtgacgg atgcctgtaa aaaacacggc ggcttctacc tcggcagtat
     5761 cggtggtccg gcagctgtat tggcgcaggg aagtattaag agcctggaat gtgttgaata
     5821 tccggaactg ggaatggaag ccatctggaa aattgaagtg gaagatttcc cggcgtttat
     5881 ccttgtggat gataaaggaa atgacttctt ccagcagata caactcacac aatgcacccg
     5941 ttgtgtgaaa taaacagagc cgcccttcgg ggcggttttt ttacatggca cgaaagacca
     6001 aacgtttgtt atcaaatggt aaataatgag tgagctaaaa gttgcttaac gaaagcaaaa
     6061 cagaaagaaa aaattaatca ggtgaggagc aggccatgaa tacagtacgc agcgaaaaag
     6121 attcgatggg agcgattgat gtcccggcag ataagctgtg gggcgcacaa actcagcgct
     6181 cgctggagca tttccgcatt tcgacggaga aaatgcccac ctcactgatt catgcgctgg
     6241 cgctaaccaa gcgcgcagcg gcaaaagtta atgaagattt aggcttgttg tctgaagaga
     6301 aagcgagcgc cattcggcag gcggcggatg aagtactggc aggacagcat gacgacgaat
     6361 tcccgctggc tatctggcag accggctccg gcacgcaaag taacatgaat atgaacgaag
     6421 tgctggctaa ccgggccagt gaattactcg gtggcgtgcg cgggatggaa cgtaaagttc
     6481 accctaacga cgacgtgaac aaaagccaaa gttccaacga tgtctttccg acggcgatgc
     6541 acgttgcggc actactggcg ctgcgcaagc aactcattcc acaacttaaa accctgaccc
     6601 agacgctgag tgaaaaatcc cgcgcatttg ctgatatcgt caaaatcggt cgtacccact
     6661 tgcaggacgc cacgccgtta acgctggggc aggagatttc cggctgggta gcgatgctgg
     6721 agcataatct caaacatatc gaatacagcc tgcctcacgt agcggaactg gctctgggcg
     6781 gtacagcggt gggtactgga ctaaataccc atccggaata tgcgcgtcgc gtagcagatg
     6841 aactggcagt cattacctgt gcaccgtttg ttaccgcacc gaacaaattt gaagcgctgg
     6901 cgacctgtga tgccctggtt caggcgcacg gcgcgttgaa agggttggct gcgtcactga
     6961 tgaaaattgc caatgatgtc cgctggctgg cctctggccc gcgctgcgga attggtgaaa
     7021 tctcaatccc ggaaaatgag ccgggcagct caatcatgcc agggaaagtg aacccaacac
     7081 agtgtgaagc attaaccatg ctctgctgtc aggtgatggg gaatgacgtg gcgatcaaca
     7141 tgggtggcgc ttccggtaac tttgaactga acgtcttccg tccgatggtg atccataatt
     7201 tcctgcaatc ggtgcgcttg ctggcagatg gcatggaaag tttcaacaaa cactgcgcag
     7261 tgggcattga accgaatcgt gagcgaatca atcaattact caatgaatcg ctgatgctgg
     7321 tgactgcgct taacacccac attggttatg acaaagccgc cgagatcgcc aaaaaggcgc
     7381 ataaagaagg gctgacctta aaagcggcgg cccttgcgct ggggtatctt agcgaagccg
     7441 agtttgaccg ctgggtacgg ccagaacaga tggtcggcag tatgaaagcc gggggttaat
     7501 ctgcaacata caggtgcagc cgtggaatga ttaaacgaag cggctgcgcc tgtggtttat
     7561 aacggtgctg cacattgtcg gcatcgtaat ttaacaactc accaacgtcc ggcacgcccg
     7621 cgccattatc ccgattaatc agcgcaatca gcggcgtagg gcaggcgtgt tgtacttgtt
     7681 tttgatctcc cttataccag acgcgggcaa tcggctgcac cttcaccgga cgtttgattt
     7741 ttaacttcgc gttctgtggc agggcagcga tatcctgata ttctcgctcc agttttcttt
     7801 gccactcctc gcgcgtccac ggtgcgacac tgcgtggtga tttcaggctt ttttccagct
     7861 gtgccaggac ttcatcacga tgcaaatttt taatgatatg tttattagcc cagccaaaac
     7921 gtaaagtggc ggggtcgtgc agaacggtga gcgtgcggta agcattaagg gtaatcagcc
     7981 ccggaaaatg acgatgtacc cattcaaaac gtgccacgga ggggagttct gattcaaccg
     8041 tgacgatatg ctcgaacgtg gtcttgagtt tattgatgtg ctgaatatga ctgaccaacg
     8101 ctgcttgcga aaagttatcg acctggtaac acaacacgcc aggcagacgg acggcggctt
     8161 tgctgctgcg attttcggac tgttgttgaa taaataaatg gcggaaatga cgcaacgcca
     8221 gcgactgcgc gtcgttgccg agatgttgtt ttacctcaat acgattaagc ggattatgct
     8281 catcctcctt ttttacctcc ggcaaagaga acacgcgggc aaccaatagc ttgtgttgct
     8341 caagatgagc ggcaaatgca gccagctctt gttccatctg gcgaaaagta gtgttgagtc
     8401 ggtctacgag atcgtaacgc gccataatat taatcacttt agttacaaca tccttatttt
     8461 atagcacagt ggtggtgact tgaccaccgt gcgcatagtg tcaggcagag gtaaattgcg
     8521 ggatgttatg ccataacggc cagctgaacg agaagcgggc accccccagt tcgctggtgt
     8581 cacaattaac cgtaccgccc attgccagtg ctatagagtg gacaatggcc agccccagcc
     8641 cgcagccgcc ggttgagcga tcccggctgg gatcgaggcg aacaaaaggt tcaaagatat
     8701 gttcgcggtt ttctggggca atccctgggc catcatcctc aacaattaat gtcgctctat
     8761 tccccgacag tagcaggctg gtttcaaccg ttgagtgaca gtagcgcagg gcgttattga
     8821 gcaaattatc cagcacgcgc tccattaagc gcatatccaa cgcagcataa tggccttgcg
     8881 cgagcgtttt tatccgtacc gttttatcgg gcgttactgc ctgaatatct gccagatgcg
     8941 ttgacagcca caacggcagg tctggttcgc taagatgaag ctcattttgt gggcgatcga
     9001 gccgggcata agtcagcagc tcttcaatta aagcttcaag ttgactgata tcgcgattca
     9061 acgcctggga ttcggcggcg ctcaggttat cgctcatctc cagtcgataa cgcaggcgca
     9121 ctaacggtgt tcgcagttcg tgagcgatac cgtcaataag ctgttttttg ctggcaatca
     9181 aggcgttgat attgtccgcc atctggttaa atgcgacgcc aagtcgttca aagctcgaac
     9241 cttcatcaaa gtggatacgc tcactgagat gcccatcgcc aaatcgttgc gccgctgctt
     9301 ccagttttaa catatcctgc cagtgcggac gcatccagat aaacaccgga aaggcgaggg
     9361 aaatggcaat aaaagcgatc agggcgatat ccagcaatcg catctgatgg aggtaataaa
     9421 gataaggaac aggaccaact gccagcacgt agtggctgcg tgggatacgt tgcaaaaaag
     9481 tgtactgatc gtccagagcg acaatttcgc cgccacgcag ccggtgcatg gaaatatcat
     9541 caagatgata tttactcagc ggctcgacac gcagatcgaa agagagattt aaatccatct
     9601 ctttcagcgt cttaccccag tcgtgtgggg ggatctcacg caattcgctg cgcatcagat
     9661 acagcgaact gttcatcaaa tcatccagcg actgtttgcc tgcgcgttcg gcggtaaatt
     9721 tgtacaccag cccgaccagc agagacatca caaggaagca gacaaacaat aacaggtaaa
     9781 actggataaa cagttttttc atcgcttatt cccacgcatg aggcgcaaaa agatagcctt
     9841 tgttacgcac ggttttaata cgataaggtt ctgcggcgtt atcgagcagt ttttttctta
     9901 accgcgaaat agccacgtcc acgctacgat ccagcccgtc ataactgacg ccgcgtaaat
     9961 ttttcagcaa tgcatcgcgg tccatgattt gcccggcatg ggtagctaat tcccacaata
    10021 attcgaaatc agctgtcgag agcgagattt cagtgttagc cagggagact acgcggttga
    10081 tgggatcgat ggtcaacgtg ccgaaatgca gggctttgta gggagtcaga gacgtttcct
    10141 gaataccttt ggtcactgtg gcttgctcat tctgacgcaa atgcaagcgt aaacgcgcta
    10201 acaaaacagc agggggcgtc gttttgagaa tatagtcgca ggcgcccatt tccagtgcca
    10261 ggatgtggtt catatcgcta tcgagagagg ttagaagaac aatcggtccg gaccactttg
    10321 cgcgtaaatc acgacaaatg gtcatgccgt ccttgcctgg tagcatgatg tcgagtaaca
    10381 ccaaatccgg attttctcgc agaatagttt cttcggcccg gtcgccgcgc ggctctacgg
    10441 taacctgcat atcatgtttt gccaggtatg cggcaatcag tgcaccgact tccgcatcat
    10501 cttccacaaa tacgatagtg ttcataacat tcacggtaga tataaaaacg tcacaataca
    10561 ccgcactgtt tttacttact atcaatcgtt tctaaacaag gtttcatcag gtattctact
    10621 gatgacttgt tatggaagtg ttaaggtaaa aagatggggc tggtaatcaa agccgcgctc
    10681 ggtgcgctgg tagtgctgtt gattggtgtt ttagcaaaaa cgaaaaatta ttatatcgcc
    10741 gggctgattc cacttttccc gacctttgcg cttatcgcgc attatattgt tgccagcgaa
    10801 cgcggcattg aagccttacg cgcaaccatc atttttagta tgtggtcgat tattccctat
    10861 tttgtttacc tggcgtcgct atggtatttc accggaatga tgcgcttgcc cgctgctttt
    10921 gttggctctg ttgcgtgctg gggaataagc gcatgggtgt tgattatatg ctggattaag
    10981 ctgcattaac gcagatgacg accgccatca agtgggagac tgcgtccggt gacaaagcag
    11041 ctggtaagta agtaatcgac caggtcgatc acttctttct cgccaggcgc ggttttcatc
    11101 agtgatttat tcagcgcctg ttgtcgatat tcggcatcat catgttcatt aaacaggatc
    11161 agcgatggcg caatagaatt cactttcact tccggtgcca gcttgcgggc aaacgagcgg
    11221 gtcatattat ccagtgccgc tttgcttgca gcataggcaa tatgtttgtc gctaccgcgc
    11281 tccaccacat aatcggtaaa gtgaatgata tcgctggcgg cgtgtccgtg cccacgcagt
    11341 aatctttcca gagcatggtt gagcaggtat ggggtattaa cgtggatctg catcatgcaa
    11401 gccagtacgt cggtcagtgg cgctcccggt ttttccgcca tccacgcgct ggcgttatgc
    11461 aaaatagcac gcagaccatg ggtgcttttt agtacttcat cggcaaacgc catcacaccg
    11521 tcgttggtcg aaaaatcagc ctgaatacac tgcgcacctg ccttaatcag tccatcaatg
    11581 gcgggatagt gcgtccgata gctgacaatc accggttgct tttgattaat gaaatgccat
    11641 gcgagggcga ggccgatgcg gcgacctccg ccagtaatta atattggcaa ggactgggtt
    11701 ttacccatcg ttatctcctt tgctatccaa cgatgggatg aacgttatcc caccagcatc
    11761 caggtcgccg gaactgaggc aattaacagc atgccaatca gcaccatttc ctgacggttc
    11821 agtacattat catgtgtatg cgttttacgc gcgtacagga aaaccagcag ccccggcgca
    11881 tacagaacaa cggataacag caagtgcatt ggaccagagg cgtacagtaa ccataagcca
    11941 taaatgcagg caccgacacc taccgcctta tgcagtggac gggttgcgat tttcagcaag
    12001 aacgcgccga ccaggaaata gggcaccaga atcatttctg aggcgatggt cagcaatgtg
    12061 ttgtaatcgg aaccggtcag ccagatgagc accagacaaa tttgcacgca gatgttggtc
    12121 agccacagtg aagcggatgg cgcagcttgt gcgttctggc gcgcaaaaat gcgcgggaat
    12181 gctttatgag tggctgcaag gaacggaact tctgctgcca tgatggtcca gcttaagtac
    12241 gcgccgcaaa cggaaacgat caaaccggca gcgatgatga tttcgcccca cggtcccatc
    12301 atttccacca tcagaccggc cattgacggg ttacgaattt cagccagttc aggacgtgcc
    12361 accacgccca gcgaaagcag cgttaccagc aaataaacgc ccagagcgga gagaaccgcc
    12421 agcagtgtcg ctttgccaac atcacgctta ttacgcgcac gcgcagaaac aaccacggca
    12481 ccttccacac caatgaaaac ccacagggtg atcagcatgg tgtttttcac ctgttcccac
    12541 acgggtacgc caagtgcaag tccggtgaag tcgagcttga aggtatccag tttgaacatc
    12601 atcatcgcca gcacaacaaa cagacccaac ggcaacaatt ttgccagtgt cgccacaagg
    12661 ttaatgctgg cagcggtttg caccccgcgc aggatcaaaa agtgaacaat ccacaataaa
    12721 gccgatgcac cgacgatcga ttgccaggta ttaccatcgc caaacaggcg caattccggc
    12781 gtgtccgtaa aaaagcttaa cgcggaaaaa acgatcacca gataggagac gttggcgatg
    12841 accgcgcaca gccagtatcc ccatgcggaa caaaagccga ttagctcgcc aaacccttcg
    12901 cgggcatagg taaagatacc gccgtcaagt tcggggcgaa tgcgcgtgag gatcagcatg
    12961 gcaaaggcca gcaataaaat gccagcgcca gtaatacccc agccgatgag cagtgctgcc
    13021 gggctggcaa ctgccgccat attttgcggc agactgaaaa cacccgcgcc cagcattgag
    13081 cttaatacca gcgcggtgag tgcgctcagt cccagtttct tttccatcga tatccagtga
    13141 ttaaaacgtg acaagaagaa taaatattag ggcaaaacgc ataaaaatgg agtttgccac
    13201 tgaagcgcgg gattttacga agagatgtgt ctgcatgcaa tggtcgcagg gaaatttatg
    13261 actaaaaatg cgaaaggcgg catcaagccg ccttatagga gtaactgaat agttatttat
    13321 acagatctgc gctgacggtc aggttattac cacgttcctg ccactggcgg gtgatgtggt
    13381 aatacttagc acctttcttc gcggcacgtt tcgcaacctg ataggagact tcggtcatgt
    13441 tgccgtagtt gccagagaat ttgatgctgt cgaacgggac catcatcgct gctgtcgctt
    13501 tgttcagttc ttcgacttta gtgccatccg ggagcgtgac ggtgtaacgc ccgccttttg
    13561 atgactgggt ttcaaagaag cgaccgactt cagaacttgg tgatgcggta gtcgcaacac
    13621 ccgggatctc aactttcttc gcggcttcgc caccggcagc cagagctgca cgtcctgctt
    13681 cggaatctgc cgggattaca tccgggctct ggacgatacg tttcttagca tcttttttat
    13741 agatgaatgc agtaatacgc tggttaccgc cctggttggc atcgatttga cgaacaatgt
    13801 agaaagagta ggcacctttc gcttttgccg ctttggtgat ggcatcattg acttctggct
    13861 ggctacgata gaagccctgg acggtgaccg tgtcaaacgg ttcaatcaga acagcctgat
    13921 ctttcggcag ttcgacaaca ccgttaatta cgcgattact tgtttcttct gctttttcag
    13981 catcggcttt atagaggtca gcgaccacac gccagttacc gctgttacca aaatcagaag
    14041 tgtcgacaac ataaaaagag gcggcacctt ctttatctgc gcgacgagaa acggctttca
    14101 ccgcttcgcc aatagcatta aaacgaccgg taaccactac acggtcaaaa ggtttaaccg
    14161 ctgccgcttg ctccggtgtc agttctgttg ctgcgttaac ggagaatgcc gtagcagaaa
    14221 gcagtgccga cgccaggagg gtgttcttaa gcttcataaa aataatcctt cgccttgcgc
    14281 aaaccaggta ctggtattgt tattaacgag aaacgtggct gattattgca tttaaacggt
    14341 gtaactgtct gcgtcatttt tcatatcaca ttccttaaac caattttaat tctgctcaaa
    14401 tgaccgtcta tgcttaaaaa acagccgtat caccatcaat actactgaag caactgaatt
    14461 gtataagtta atttaatgtt aagtagtgat ttgtgccggg gcgatgtctc gttttacccg
    14521 gccgccgaag acaattatca gtctttatcc ggcgttctaa ggtgtttatc ccactatcac
    14581 ggctgaatcg ttaatatttt gcgagttcac gccgaaatac tgatttttgg cgctagatca
    14641 caggcataat tttcagtacg ttatagggcg tttgttacta atttatttta acggagtaac
    14701 atttagctcg tacatgagcg gcttgtgtgg ctcctgacac aagcaaacca tcatcaataa
    14761 aaccgatgga agggaatatc atgcgaattg gaataccaag agaacggtta accaatgaaa
    14821 cccgtgttgc agcaacgcca aaaacagtgg aacagctgct gaaactgggt tttaccgtcg
    14881 cggtagagag cggcgcgggt caactggcaa gttttgacga taaggcgttt gtgcaagcgg
    14941 gcgctgaaat tgtagaaggg aatagcgtct ggcagtcaga gatcattctg aaggtcaatg
    15001 cgccgttaga tgatgaaatt gcgttactga atcctgggac aacgctggtg agttttatct
    15061 ggcctgcgca gaatccggaa ttaatgcaaa aacttgcgga acgtaacgtg accgtgatgg
    15121 cgatggactc tgtgccgcgt atctcacgcg cacaatcgct ggacgcacta agctcgatgg
    15181 cgaatatcgc cggttatcgc gccattgttg aagcggcaca tgaatttggg cgcttcttta
    15241 ccggacaaat cactgcggcc gggaaagtgc caccggcaaa agtgatggtg attggtgccg
    15301 gtgtcgcagg tctggccgcc attggcgcag caaacagtct cggcgcgatt gtgcgtgcat
    15361 tcgacacccg cccggaagtg aaagaacaag ttcaaagtat gggcgcagaa ttcctcgagc
    15421 tggattttaa agaggaagcg ggcagcggcg atggctatgc caaagtgatg tcggacgcgt
    15481 tcatcaaagc ggaaatggaa ctctttgccg cccaggcaaa agaggtcgat atcattgtca
    15541 ccaccgcgct tattccaggc aaaccagcgc cgaagctaat tacccgtgaa atggttgact
    15601 ccatgaaggc gggcagtgtg attgtcgacc tggcagccca aaacggcggc aactgtgaat
    15661 acaccgtgcc gggtgaaatc ttcactacgg aaaatggtgt caaagtaatt ggttacaccg
    15721 atcttccggg ccgtctgccg acgcaatcct cacagcttta cggcactaac ctcgttaatc
    15781 tgctgaaact gttgtgcaaa gagaaagacg gcaacatcac tgtggatttt gatgatgtgg
    15841 tgattcgtgg cgtgactgtg atccgtgcgg gcgaaattac ctggccggca ccgccgattc
    15901 aggtatcagc tcagccgcag acggcacaaa aagcggcacc ggaagtgaaa actgaggaaa
    15961 aatgtgcctg ctcaccgtgg cgtaaatacg cgttgatggc gttggcaatc atccttttcg
    16021 gctggatggc aagcgttgcg ccaaaagagt tccttggaca cttcactgtg ttcgcgctgg
    16081 cctgcgttgt cggttattac gtggtgtgga atgtatcgca cgcgctgcat acaccgttga
    16141 tgtcggtcac caacgcgatt tcagggatta ttgttgtcgg agcactgttg cagattggcc
    16201 agggcggctg ggttagcttc cttagtttta tcgcggtgct tatagccagc attaatattt
    16261 tcggtggctt caccgtgact cagcgcatgc tgaaaatgtt ccgcaaaaat taaggggtaa
    16321 catatgtctg gaggattagt tacagctgca tacattgttg ccgcgatcct gtttatcttc
    16381 agtctggccg ggctttcgaa acatgaaacg tctcgccagg gtaacaattt cggtatcgcc
    16441 ggtatggcga ttgcgttaat cgcaaccatt tttggaccgg atacgggtaa tgttggctgg
    16501 atcttgctgg cgatggttat tggtggggca attggtatcc gtctggcgaa gaaagttgaa
    16561 atgaccgaaa tgccagaact ggtggcgatc ctgcatagct tcgtgggtct ggcggcagtg
    16621 ctggttggtt ttaacagcta tctgcatcat gacgcgggaa tggcaccgat tctggtcaat
    16681 attcacctga cggaagtgtt cctcggtatc ttcatcgggg cggtaacgtt tacgggttcg
    16741 gtggtggcgt tcggcaaact gtgtggcaag atttcgtcta aaccgttgat gctgccaaac
    16801 cgtcacaaaa tgaacctggc ggctctgatc gtttccttcc tgctgctgat tgtatttgtt
    16861 cgcacggata gcgtcggcct gcaagtgctg gcattgctga taatgaccgc cattgcgctg
    16921 gtattcggct ggcatttagt cgcctccatc ggtggtgcag atatgccagt ggtggtgtcg
    16981 atgctgaact cgtactccgg ctgggcggct gcggctgcgg gctttatgct cagcaacgac
    17041 ctgctgattg tgaccggtgc gctggtcggt tcttcggggg ctatcctttc ttacattatg
    17101 tgtaaggcga tgaaccgttc ctttatcagc gttattgcgg gcggtttcgg caccgacggc
    17161 tcttctactg gtgatgatca ggaagtgggc gagcaccgcg aaatcaccgc agaagagaca
    17221 gcggaactgc tgaaaaattc ccattcagtg atcattactc cggggtacgg catggcagtt
    17281 gcgcaggcgc aatatcctgt cgctgaaatt actgagaaat tgcgcgctcg tggtattaac
    17341 gtgcgtttcg gtatccaccc ggttgcgggg cgtttgcctg gacatatgaa cgtattgctg
    17401 gctgaagcaa aagtaccgta tgacatcgtg ctggaaatgg acgagatcaa cgatgacttt
    17461 gctgataccg ataccgtact ggtgattggt gctaacgata cggttaaccc ggcggcgcag
    17521 gatgatccga agagtccgat tgctggtatg cctgtgctgg aagtgtggaa agcgcagaac
    17581 gtgattgtct ttaaacgttc gatgaacact ggctatgctg gtgtgcaaaa cccgctgttc
    17641 ttcaaggaaa acacccacat gctgtttggt gacgccaaag ccagcgtgga tgcaatcctg
    17701 aaagctctgt aaccctgacg gcctctgcgg aggccgtcac tctttattga gatcgcttaa
    17761 cagaacggcg atgctttgac ctcccgctgt ttgttcaagc gcaattttga caataattgt
    17821 caacggcacg gaaagcagca tacccaccgg tcctaacaac catccccaaa aaatcaacga
    17881 caaaaatacc accaatgtgg aaagccccag cccacgcccc atgatgcgcg gctccagaat
    17941 attgccgaag acaagattaa tcagcagata tcccgccagc accagcaacg cttcgtagaa
    18001 gccattaaac accagtacct gagcgatagg ggggattgcc gcgagaactg aaccaatatt
    18061 ggggatgtaa ttaagcgcaa aggccagcaa tccccagaca aaagcgaagc gaacatcgag
    18121 tgcggcgagc atcgcccagg cgaccaggcc ggtgatgatg ctgatggctg ttttcagcac
    18181 cagataatga gacacgctgt caatcgcgcg ctgaatcgcc gccatccctt caaccggacg
    18241 cgccatcatt tgctgaaatt ttccgggcaa ttgtggcact tcgagcaaca taaacagcac
    18301 cgtcagcagc aataaaaaga ttgatgacat ggcattagat aactgcgtca ataagttggt
    18361 gagcaacgtc atcgccgcgt tcggatcaat gtaatgcgcc agttggtcaa ctgagacatc
    18421 aatccctacg cgttgcaaca acggttcaat cgcttgcagc ggcgtcataa tagagttgcg
    18481 atattgcggt aacgtccgcg tcaactcatt gagcgcggac cccagataag ctaacagcaa
    18541 caccatcgcc atcacgatga tggtcatcaa aatcgacact gccagtacac gcggcacacg
    18601 ccagcggacc atgtgttgca ccagcgggtt aagaataaca gcaataaata atgcgagaat
    18661 aaacggcacg ataatctcgg cggcaaaacg gataccgcag agaataatga ccagcattcc
    18721 caacataatg acgattttta ggccattgag cgtgatgatc ggctttgcca tgcttgtccc
    18781 cgttttttct tcctttgcta ataataatgt ctttttcgct ccttcatcat aacgaaaatc
    18841 aaaccgctca ggtaaagaag tgaaaatatt ttgagttaat tcttaagcta tgatacaaat
    18901 caggcgtgtt caactaccga ggacaattat catccgcgat gacgagaagc aacactgcgg
    18961 ataattgtaa tattatggac aatttgttca ggacgttatt ttctacattt acccatcttc
    19021 gtacctcatc cactatcttg ctcgtaggtg agcagcactg gcgcaacgcg ctttagtcct
    19081 ttcttctgcc taagcttgcg ctatgcgcgg ggcttgatca ctttactttg gtttcgctga
    19141 attaagcgaa aattaaaata attctcttgc aggagaagga caatgtatat ttattggatt
    19201 ttattaggcc tggctattgc tacggaaatt accggtacgc tgtcaatgaa atgggcgagc
    19261 gtcagtgagg gaaatggcgg ctttatttta atgctggtga tgatttctct gtcgtatata
    19321 tttctctctt tcgccgttaa aaaaatcgcc ttaggcgtag cttatgcgct gtgggaaggt
    19381 attggtattt tatttattac cttgtttagc gttttgttat tcgacgaaag tttatcgctg
    19441 atgaaaattg ccgggttaac caccctggtg gccgggattg tgttgatcaa gtcaggtacc
    19501 cgtaaagcgc gtaaacctga actggaggtg aaccatggcg cagtttgaat gggttcacgc
    19561 cgcctggctg gcattggcaa tcgtgctgga aatcgttgct aacgtctttt tgaaattttc
    19621 tgacggcttt cgtcgcaaaa tatttggctt gctctctctg gcggcggtgc tggctgcctt
    19681 tagtgcgctt tctcaggccg ttaaagggat cgacttgtct gtcgcttatg cattgtgggg
    19741 cgggtttggt attgccgcca cgttagccgc aggttggatc ttgtttggtc aacggttaaa
    19801 tcgtaaaggc tggattggcc tggtgctgct gttggctgga atgatcatgg tgaaacttgc
    19861 ctgatgaaca cgctgcccgc gctgtcgggc agcgtttgaa cattattttt gcgacagttg
    19921 atccagcttg tcgcgaaaac cggtaacaga aatggcccgg ttatcggcgc gccagcgatc
    19981 tttcgcggca ggagccgagc tttgcacccc aattaattgc cagccgtcat cggtatgcaa
    20041 catcagaggc gaaccgctgt cacccggcaa ggtatcgcac tgatgcgaca tcaccgacgt
    20101 ttgcgcccag ccagtcactt cacagttttg atgactgtac aacgtatcga gatgatcttc
    20161 agggtagccc gcctgagtca ctttacgacc agccgctttt aatgcggcgg taagcgcggc
    20221 tttatctccc tcaaataacg gcaacggtgt aatgccagaa gggggattac gtagcacaat
    20281 caaaccgaag tcccacggcg cagctgcggg aggtacaatc cagccatccc catctgcttt
    20341 taaccgcttt cccagtgttg gatcaacgcg gccttctatg tcgtggatct catagcgcca
    20401 aagaccttta tttgacacga aacgcagcgc taccgcttta tcggctttac cctttggcgg
    20461 cgtcaataaa cagtgtcctg ccgttaatgc cagattgggt gcaatcagcg tcgccgtaca
    20521 taaattgccg ctggccgttt ccagttggcc aaccgcatcc cacggtgatt gggtcgtgtc
    20581 attcactggc acacgatcat catgaccaaa aaacagggtg ctgacctcat cgtttgccga
    20641 tttggcaacg tctggtttat ctgcaaacac aaaagcagac gtcaaactaa ttgcacccaa
    20701 cactacagca atggttgtac gcatatcaca ctctggtggg taattatgat tattaaaagc
    20761 aaaccctcat aaatactata gacgggacgg gatgaaagtg ggagtaaaat cagataacta
    20821 cgatcaggaa agataaaaac gggcggcaat cagtgcgcat aaaataagga tgataagaat
    20881 gaactcaaag cgataacggc ccaccatact accctccgct aaaggcggtg ctaaacgtta
    20941 cccgcttagc accgtaattt tcaggcgcga gggggcgcgc cagcattact gttgaaaact
    21001 tacgctgcgg gttgtgcagc aggttttgcc ggttgctgat ggctgtgttt cttggcgtgt
    21061 ttcttcgctg cctgcgcttt ttgttcagga gctttctgtt cagctttggc atttttatga
    21121 tgctttttag ccgcctgcgc tttctgggca ggtgctgctt tatgctgttt tttatgatgt
    21181 gtggttttcg caggcgctgc tttggtggtc gtcgcagtcg gagcaggtgt ggtcgcagtc
    21241 tctgcagcaa aggcagcaga agacagaccc atagcagcgg caacaaccag agctaatact
    21301 tttttcattg tcataccctc aatttgtttt ttcatttaac cccactgcgg ggccgttgaa
    21361 ataactatat ccctgggaat ggcagacttc cgtgagtggt tggtttcagc gtgtaacgat
    21421 atgtacaaac gctgaataaa ttacagcatt gataataacg tattgtgact taaggaaaat
    21481 ttagctacca ataaagtagt cttgatcgtg ttaactattt tactacttac aaagcggtag
    21541 aataactgcg catcagtaat aaatgacaca cagcaaaatg aatccgttta tttgggtact
    21601 tataatcctg atgacgctag atgcgctgcg ggaattggct ggcgcttcgt ctattttagg
    21661 atggctatta acgttggttt gagcaggcaa taagtccgga cgggtattta cctcagtccg
    21721 gacttatttt ttcaggcgtg cagacgatga tgcaaacgcg tcccgaccag cagagcaatg
    21781 accagcatca gagcaataaa tgcgccgacg ccgttccagc catagttatg ccagaaaaca
    21841 ccacccagcg tcccggcaat actcgacccc agatagtaac tgaacagata cagcgaggag
    21901 gcctggcctt tagcgcgttt tgcgcggggg ccgatccagc tgctggcaac ggagtgggct
    21961 gcgaagaatc cggctgagaa gagtaacatt ccggcaaaga tcagccacag cgagctgaat
    22021 aaggtcatca gtaaaccaaa cagcataacc cccgtcgaaa acaacatcac cggaccgcgt
    22081 ccatagcggg cggtcatggt tccggctttg ggtgagctcc atgtaccggt caaataagcc
    22141 agcgataata agccaaccac ggcctgactg acatgccagg gggaaagcat caacctatag
    22201 ccgatgtaat taaacagcgt gacgaacgac cccatcagca aaaagccttc tgcgaacaat
    22261 aacggtaatc cccggtcacg ccagtgcaga cgaaagttga taaataacgt cttagggcgc
    22321 agcgaagtcg ggcgaaaatg gcgtgattca gggaggattt tccagaacat caacgccgag
    22381 gccagcgcga aacaaccgat tgccgccaga gcaattcgcc agttaaaaaa gtccgtgaag
    22441 acaccgctaa ttaagcgtcc gctcatgccg ccaattgagt tgccgctgat atacaacccc
    22501 attgaaaagg ccacgaaact agggtggatt tcctcgctaa gataagtcat gccaacggct
    22561 gccacgccac ttaacgaaag cccaatcaag gcgcgcataa tcaaaatgcc gtgccagctg
    22621 gtcatcattg tcgaaagtaa cgtacaaatg gaggccaaca gtagcgccgt gaccatcact
    22681 ggtttgcgac caatggcatc ggatagcggg ccagtaaaca gcaaaccgat agccaacatc
    22741 gccgttgaaa tggacagtga aatactactg gtcgcaggcg ttaaaccaaa ctcctgcgaa
    22801 agcaccggaa ggataggttg cacacaatag agaagtgcga aggttgccag tccggcagag
    22861 aaaagcgcca gggtgacgcg cataaattgc ggcgtaccgc gtttaataaa atgatttggc
    22921 tgagaaatgc tttgcttgtc agtgtcgctt gccggagcgc catcaacagt tgtagtacgg
    22981 ctcacttgaa atccttgcta aatatgcctg tggatcaggc ttatgaatag attaggaaaa
    23041 tcgaattgtt ctgtctaata tattaataat ctcaaataag atgttttaaa tatgaatatt
    23101 gaacttcgtc atctgcgtta ctttgttgct gttgcagaag agctgcattt cgggcgcgcc
    23161 gctgcccgcc tgaatatttc gcaaccaccg ctaagtcagc agattcaggc gctggagcaa
    23221 caaattggtg cccgactgct ggcacggacc aatcgcagtg tattgctgac ggaagcagga
    23281 aaacagtttc ttgtagatag tcggcaaatc ctgtctatgg tggatgatgc tgccgctcgc
    23341 gctgaaaggc tgcatcaggg cgaagcgggg gagttgcgca ttggttttac ttcgtccgct
    23401 ccttttattc gggcggtgtc cgatacgtta tcgctgtttc gccgtgatta tcccgatgtc
    23461 catttacaaa cccgcgaaat gaacactcgc gagcaaatcg ctccgctcat tgaaggaacg
    23521 ctggatatgg gattgctgcg taacacagcg ttaccggaga cgcttgaaca cgcagtcatc
    23581 gtccatgaac cgcttatggc gatgatcccg cacgatcatc ccctggcaaa taacccgagt
    23641 gtaacgctgg ctgaactggc gaaagaaccc tttgtctttt ttgatccgca cgtcggaaca
    23701 gggctgtatg acgatattct cgggctgatg agacgttaca atttgacgcc cgtcatcact
    23761 caggaggtgg gtgaggcaat gaccatcatc ggtctggttt ccgctggtct gggtgtttca
    23821 attttgcctg cgtcatttaa acgtgttcag ctcaacgaaa tgcgctgggt gccgattgct
    23881 gaagaggatg cggtttctga aatgtggttg gtctggccga aacatcatga acaaagtccg
    23941 gctgcgcgta actttcgtat tcatctgctg aatgctctca ggtgagggaa atttcagcga
    24001 aaaagcccga aaaatgtgct gttaatcaca tgcctaagta aaaatttgac gacacgtatt
    24061 gaagtgcttc accatagcct acagattatt tcggagcgcg aaaatatagg gagtatgcgg
    24121 tggttgctga aaaccagcct gggcacattg atcaaataaa gcaaaccaac gcgggcgcag
    24181 tttatcgcct gattgatcag cttggtccag tctcgcgtat cgatctttcc cgtctggcgc
    24241 aactggctcc tgccagtatc actaaaattg tccgtgagat gctcgaagca cacctggtgc
    24301 aagagctgga aatcaaagaa gcggggaacc gtggccgtcc ggcagtgggg ctggtggttg
    24361 aaactgaagc ctggcactat ctttctctgc gcattagtcg cggggagatt ttccttgctt
    24421 tgcgcgatct gagcagcaaa ctggtagtgg aggagtcgca ggaactggcg ttaaaagatg
    24481 actcgccatt gctggatcgt atcatttccc atatcgatca gttttttatc cgccaccaga
    24541 aaaaacttga gcgtctgact tcgattgcca taaccttgcc gggaattatt gatacggaaa
    24601 atggtattgt acatcgcatg ccgttctacg aggatgtaaa agagatgccg ctcggcgagg
    24661 cgctggagca gcataccggc gtaccggttt atattcagca tgatatcagc gcatggacga
    24721 tggctgaggc cttgtttggt gcctcacgcg gggcgcgcga tgtgattcag gtggttatcg
    24781 atcacaacgt gggggctggc gtcattaccg atggtcatct gctacacgca ggcagcagta
    24841 gcctcgtgga aataggccac acacaggtcg acccgtatgg gaaacgctgt tattgcggga
    24901 atcacggttg cctcgaaacc atcgccagtg tggacagtat tcttgagctg gcacagctgc
    24961 gtctcaatca atccatgagc tcgatgttac atggacaacc gttaaccgtg gactcattgt
    25021 gtcaggcggc attgcgcggc gatttactgg caaaagacat cattaccggg gtaggcgcgc
    25081 atgtcggtcg cattcttgcc atcatggtga atttatttaa cccacaaaaa atactgattg
    25141 gctcaccgtt aagtaaagcg gcagatatcc tcttcccggt catctcggac agcatccgtc
    25201 agcaggccct tcctgcgtat agtcagcaca ttagcgttga gagtactcag ttttctaacc
    25261 agggcacgat ggcaggcgct gcgctggtaa aagacgcgat gtataacggt tctttgttga
    25321 ttcgtctgtt gcagggttaa cattttttaa ctgttctacc aaaatttgcg ctatctcaat
    25381 ttgggccagg aaagcataac ttagactttc aaggttaatt attttcctgg tttatatttg
    25441 tgaagcataa cggtggagtt agtgatgctg aagcgtttct ttattaccgg tacagacact
    25501 tctgtaggga aaacggtggt ttcccgcgca ttgctacaag cgttagcctc ccagggaaaa
    25561 acggttgcgg gatataaacc cgtagcgaag gggagcaaag agacacccga agggctgcgt
    25621 aataaagatg ccctggtgtt gcagagtgtt tcaaccatcg aactgcctta tgaagcagtt
    25681 aatcctatcg cgttaagcga agaagaaagt agcgtggcgc acagttgccc aatcaattac
    25741 accctcattt caaacggcct ggcaaacctg accgataaag tcgatcatgt cgtggtagaa
    25801 gggactggcg gctggcgcag tctgatgaat gatttgcgtc cgctctctga atgggtggtg
    25861 caggaacaac tgccggtgtt gatggttgtc ggtattcagg aaggttgcat taaccatgca
    25921 ctgctaacag ctcaggcgat tgccaacgac gggctgccgc tcattggctg ggtggctaac
    25981 cgaatcaacc caggactggc gcattatgcg gaaatcattg atgtgctcgg taaaaaactt
    26041 ccggcaccgc tcattggtga actgccttat ctgccgcgcg ccgaacagcg tgaactgggg
    26101 caatacatcc gcttagctat gcttcgcagt gtgctggcgg tagatagagt cacggtgtaa
    26161 cgtccgcgaa attaccgacg caattacgca ggcaatcaat aaaccgggga gtagctgata
    26221 ctccccggtc atttcacata tcatcaacgt cgacatagtc ggcgcgtgcg tggtcgccgc
    26281 caacagtgtc gccatcccgg tcaatccgag taaaagcgta atttcttcgc catcaggtaa
    26341 ccataatccc aggctacgac catacaacat gccaatggca agaccgataa atagcgtcgg
    26401 tgtaaagaca ccaccgggcg cgccagaacc actactcgcc agcacggcac acagtttaca
    26461 gaggaagatc ccggcaatga tcattaacag tggcggagcg gttaaaaagg attgtacggt
    26521 gctatagccg ttgccccaca ctgcaggtgt gaacagggaa agaagaccca cgatcaaccc
    26581 gcccagtgcc agttgccagg gcggcgcaag cttgagactc acaaatccac gatgacaggc
    26641 gttcattaac gttaacaaca gtggtccgca cagacctgcc agcacacctg tactgataat
    26701 cagcgcatag tcacgagcct gaaccgtcac tgaaagttgt acgctgtaga gtaatgcgtc
    26761 gctatgatta ataagattgc taaccagcaa cgccacgacg gcggaaataa tcaccgggcc
    26821 gagagaggcc aacatcatag tgccaaacag cacttcggct ataaataaac taccagcaag
    26881 cggggcgcga taggccgcag ccattcccgc cgcagctcca caggcgatcc ataatttcca
    26941 ctcctggcgt ggcgtaaaac gttgggcaaa acacgaggct gcaagggcag ctaaaagaat
    27001 catcgcacct tcgcgaccaa ttgcactgcc gctggttact accagcagag aggcaagcga
    27061 tttaaccaga cttgctgcgt aatcaaactg accatcggtt tgcaacgctt ccatgtaatc
    27121 ggtcggcgca tgagggcgtt gctgggtaaa tttctgccag cccatcagca acaaacccgc
    27181 cgccagtccg ccgagcgccg gggttagcag ccgtcgccac ggggaaagat ttgtcgctgc
    27241 attgaccaga ctgccagagt cattattgag gaacagccac tccagtagca gcatcgcatg
    27301 acgaaacccg gcaacggcaa aggccgcaag aataccgacg attgttgcga taagcagacg
    27361 gcggaacata gtgcgcagat ccggatagat gtgtagatga tgcatgagca gaaatgggca
    27421 atgaaacacg ggcgactatt ttgcggcaaa agaggccagc cgcaaaagca gtgtgtgctt
    27481 taagagataa aaaaaacgtg acactgtaac ctattaattg ccgaattgcg tgtaatcgtc
    27541 accatccggc aataatacgg tgatcctatc gaaacagcgg tttaaccgcg acaggaatca
    27601 acagttgtga ttgccattgc gccagcgtta agcgagcgag ttcacccagt gcacggtaga
    27661 aagggtgttc tgctttttcg ataaaaacgt caagaaaacg tgttgaccac ggaaaaaggt
    27721 gccatgccag caattcttcg cattctgtct ggcgaccatt ctccgccaac cacgcagcca
    27781 tcagcagcag tgaaccaaaa ttatcttccg gttcgttttg cttcatttcg aactgaatgc
    27841 ctttctcgcg catccactga cgaagtgcca atgttgaatc gccaaacagc acagattcgc
    27901 gatccagcca gaccgatccc cacggcggag acggcagtgc ccacgggccg acaaacaaac
    27961 gctgccaggc ctgggcgtga gtctcttcac attgtgtctg aaaagccgtc accagcggcg
    28021 ctaatgacgc ttctggtaga ggccactgag tttcccagcc gtcattggtg agtaccgcaa
    28081 ccagaggtgc ggcttccgca ctctctggag cgtaataaaa cagcgcgccc aacacgcgcg
    28141 ccgcaacaga aaaattatct tgctgtgaaa aatgggtcat tcctaacatc ctgaaatgtg
    28201 cgggttgccc cgcaccaatt tgttaacctg caatagccat tcctaccgtc atgtgcaggc
    28261 cataaaagag cacacgcccg atcatctcac cgccaagaat gaggattagc cccagaacaa
    28321 gtccggcaac gtgaggttca cggcgacgaa tgagcggaca aagccagcat ccaagacctg
    28381 cgcataacaa taccacacgc cagacctgta aggaggcgta atctggtacc agtgcgctgg
    28441 cttgttgtac ggatgagtga atagacgcca gagacaaacc ttgcagcaca atcaacccgg
    28501 cacaagcgat cagcgccaga atgctaatga tggcaaatgg tgtggtatta aatgtaacgc
    28561 gggctgcacg cagaattgtg gcagcaagta ttggaccgct caacaatacc gtcaggaaga
    28621 aagccagcgt agtgtaaccg ttatgccagg ttggcacagt gtcgatttga tacacgcagg
    28681 tcatcatcca gacgaaaatg atgccgagtg ccatgctgac aagtaaccag agtttcccca
    28741 acgcttgcgg cattttaccg atgaccgcca ccagccacca taatccgcca acggcaaaaa
    28801 atatggaacc ggcagcaatc tcgttactca gaccagaagc tccgattcta ttcagcgagt
    28861 taaacgcccg cagtggcgat cccaggtgca tgacagaggc aataaacccg atgcccatca
    28921 acagccagag gaaaaacatg ccacgaataa tgtgctgacg gtctgcgtca tttttggcgg
    28981 cgaaccagcc aattccgcta acaatcagcg cgcctactac gcattgaccc aatacggtaa
    29041 agataaccag tggccattca tgccatccat ttcccatttt acacttcctc cggattagcc
    29101 agataaccag tggtatcgcc ggtcgggcgg ctgttggcgt taggtttgat aacgatattg
    29161 ggttttgtga aatgcgcgcg cggcagtggc gcgacggcag ccagagtgcc gtgcttctgg
    29221 cgcaattctt caattggacc gaactcaagt gcacgcagtg ggcaggattc gacacatatg
    29281 ggttgtttcc cctcggcgac gcgcgaataa caaccatcgc acttcgtcat gtgccctttt
    29341 tcagcattgt actgtggcgc gccgtacgga caagccatgt ggcagtagcg gcagccgata
    29401 caaacatctt catcgaccac cacaaagcca tcttctcgtt tgtgcattgc gccgctgggg
    29461 cagactttgg tacacgccgg atcatcgcag tggttgcacg aaattgagag atagtaggca
    29521 aagacattct gatgccagac gccattatct tcctgccagt cgccgccagc atattcataa
    29581 atacggcgga aactgacttc cgggccaaga tctttaaaat ctttgcacgc cagttcgcag
    29641 gttttacagc cagtacagcg gctggaatcg ataaaaaatc catattgtgt ggtcatgggc
    29701 tactccttaa accttttcga tctggacaag attgctgtgc gacgggtttc cctttgccag
    29761 cggtgaaggg cggtgagagg tcagaatacc gttaactgca gtggataggt cccccgtagg
    29821 ggatcgttcc aaccgtcgaa acctggggta tacgctggca gtggatgaat aatttcatct
    29881 tttttcaatt cccaggtatc cgcaattgtc gccagtcgtt cagaataaat ttcgattttt
    29941 cccgaaggtg ttttcaacgg gtttgcctgt ggatctacac ggaatgcgcg gaaagcgacg
    30001 tagtgttctt ccgggcattt tttcttaaag atcccggtcg ttttcatctc ctcgtagtcg
    30061 ggcatctcag ggttacgttc cttcgttttc gcatggagat atttgatcca ttcatgctga
    30121 ctgcgacctt cagtaaaggt ttgataaacg tctggtccta agcgtttggc gacttcactc
    30181 agcatccagt agatgggttt gcgttcaaat tttgctgagg ttgcgggttg ggcgaggatc
    30241 acatagccca tattcccggc agattcatga gagataaggt cttcttgctc tgttggcatc
    30301 aggtcgggta acaggatatc gcaatactta gccgaggcag tcatgaagtg gtcaatgcca
    30361 acaatcatct cgcacttgct gtcatcctga agcacctcat gggtgtgatt gatgtcgcca
    30421 tgctgattga tcaatgtgtt actggcgtag caccataaaa acttgatggg gacatccagt
    30481 ttttcttttc ctcgaacacc atcacgggtc gcggtcattt ccgtaccatg gtcgatggca
    30541 tctgtccatg taaagacgga aagccgctca tccgcgtttc tgccgggtta tttccgaaca
    30601 taacgaccag tttcgtattg gcgatatcat ccgggctgtt gccatcattg gcaccgaaca
    30661 tataactcat tgcggcactg atctgtgcgg tactgtagct gccatagcga ctgagaaaac
    30721 caccgcaaga gttcatcaga cggtacggga cgtttgagtt ggtgatgttt ccgccatcta
    30781 cgcctgttcc atagagaaca tgtacagcct cattgccgta atcttttagg atccgccgaa
    30841 gattatcact gatggtatcc agggcttcgt cccaacttat ccgttcaaat ttaccttcac
    30901 cgcgcttgcc gacgcgcttc atgggatatt tcaacctgtc aggatgattc atccgtcggc
    30961 ggatagagcg cccgcgtaaa catgcccgaa cctgatgatt accgtaaacg tcgtcacctg
    31021 tcgtatcaga ctccacccag tacacggtgt catctttcac atgcaaacgt aacagacagc
    31081 ggctcccgca gttaacggtg caggaactcc agaccgcttt ctcttctacc ggtgcctgtg
    31141 ccgctcggac catttgggaa aacggcaaag tgaaagcact gcttgccagc gcgagactgc
    31201 caagtgcgga ggttttcatc agacttctac gactgatttc agccgacatg agcgcctctg
    31261 tgttatggat tttcatcatt actcacttat tgcttttcaa acaaaatgtc atgtcaaaat
    31321 ttatggccgt agtgggttat attttttcga tctcgaccag attagtgtgc tgcgggtttc
    31381 ccttcgccag tggtgaaggg cgcagagtgg ttagcgtatt cacacagccg ccgtggtcga
    31441 ttttatcgcc agacatattg gcctcgtgcc aggctccctg gcccatagca ctaactccag
    31501 ggagaatgcg tggtgtcact ttggctggta gccgaacttc gccacgatgg ttaaacaccc
    31561 gcaccatatc gccgttggca atcccacgtt tctgtgcatc tatagggttg atccacactt
    31621 cctgacggca ggctgccttc aggacatcaa tgttgccgta ggtcgagtgc gtacgggatt
    31681 tgtaatggaa accaaacagt tgcagtggga aggtactacg ttcaggggag tcccaccctt
    31741 caaaggttgt actgcaccca ttttgttgga cgatgaaatg gaatagcccc taatatgtca
    31801 aagccaaaat acccttttga aaagcgcctt gaagtcgtga atcactactt cacaactgat
    31861 gatggttaca ggatcatctc ggcacgtttt ggtgtccccc gaacccaggt caggacatgg
    31921 gttgccctct atgaaaaaca tggagaaaaa ggtttaattc ccaaacctaa aggcgttagt
    31981 gctgatccag agttgcgtat taaggtcgtg aaagctgtga tcgagcagca catgtccctt
    32041 aatcaggctg ctgctcactt tatgcttgct ggtagtggtt ctgtagccag gtggctgaag
    32101 gtctatgaag agcgcggaga agctggttta cgcgcgctca agattggcac caaaagaaac
    32161 attgcaatat cagttgatcc agaaaaagcg gcatcagcat tggagctgtc aaaagaccga
    32221 cgcattgagg atcttgaaag gcaagttcga tttcttgaaa cgcggcttat gtatctaaaa
    32281 aagctgaaag ccttagctca tcccacgaaa aagtgaaagt actcaacgag ctaaggcagt
    32341 tttatcctct tgatgagctt ctcagggctg cggagatacc gcgcagtacg ttttattatc
    32401 atctaaaggc tctcagcaag cctgacaagt atgcggacgt taaaaagcgt attagtgaga
    32461 tttatcacga gaatagaggc cgatacggat accgtagggt aacgctgtct cttcatcgag
    32521 aagggaaaca gattaaccat aaagctgttc agcgcctgat gggaaccctc tcacttaaag
    32581 cagcgattaa ggtcaagcga taccgctctt acagaggaga ggtagggcaa accgccccta
    32641 atgttctcca aagagatttc aaggctacgc ggccaaacga gaagtgggtt accgatgtta
    32701 ctgaatttgc agtcaatggg cgcaagctgt atttgtctcc agtaatagat ctcttcaaca
    32761 acgaagttat ttcttacagc ctttcggaaa gaccagtgat gaacatggtt gagaatatgc
    32821 tcgatcaggc attcaaaaag cttaatcctc acgagcatcc tgttctgcac tctgaccagg
    32881 gatggcagta tcgtatgaga agatatcaaa atatccttaa agaacatggt attaaacaaa
    32941 gcatgtccag aaaaggcaat tgtctggata atgctgtggt ggagtgtttc tttggaacct
    33001 taaagtcgga gtgtttttat cttgatgagt tcagtaatat aagcgaactg aaggatgctg
    33061 ttacggaata tattgaatac tacaacagca gaagaattag cctgaaatta aaaggtctga
    33121 ctccaattga atatcggaat cagacctata tgcctcgtgt ttaactgtcc aactttttgg
    33181 ggtcagtaca ggttgaggca taaaccggca gtgggcttat cacttcatct ttttccagtt
    33241 cccaggtacg ggcaatttcc gccagcttgc tggaataaat ttcaatctta ccggaaggcg
    33301 ttttaagtgg atttgcctcg gggtcgtcac gaaatgcttt gtaggcgaca aaatgaccat
    33361 tgggatcttt acgcttatag atacccattt tttttaattc gtcgtaagac ggtaacgccg
    33421 gatctttggc aagcattttg gcgtacagat gttgtaacca ttgttcttgc gtgcgacctt
    33481 cagtgaactt ttgatagacg tcaggtccaa gacgtttcgc gacttcactc aggatccagt
    33541 aaattggttt gcgttcgaat ttttcgctgg tgacaggctg gaggaaaatg agatatccca
    33601 tgttaccggc gtagtcgtta ggaataatat cttcctgctc aacggtcatt aggtctggca
    33661 gcagaatgtc ggcatatttt gccgatgagg tcataaagtt ttcgatgacc acaatcattt
    33721 cgcatttcga ttcgtcctgc agaatttcat gcgttttgtt gatgtcagaa tgctgattaa
    33781 ccagggtatt tcccgcgtag ttccagatga acttaatggg cacatctagt ttatctttgc
    33841 cccggacgcc gtcgcggatt gccgtcattt gcgggccatg atcgatagca tctgtccaac
    33901 tgaagcagga gattgacgtt ttgaccggat tatccagcac cggcaggcgt tcgatggtaa
    33961 tggtgtaggt cgattcacgc gcaccactat ttccgccgct gatgccgaca ttgcccgtca
    34021 aaataggcaa catagcaata gcgcgtgccg tcagctcgcc gtttgcctgc cgttgcggcc
    34081 cccagccctg gcagatataa gcgggttttg ctgtgccaat ttcacgcgcc agtttgatga
    34141 tacggtctac cgggatacct gtaatttgcg aagcccactg cggcgttttc gctgttttat
    34201 catcaccttc accaagaata taggctttat agtgaccatt tttgggtgca tctgcgggta
    34261 aggttttttc gtcatagccg acgcagtatt tatcgagaaa aggttgatca acgagatttt
    34321 cgttaatcaa tacccaggca atacccgcaa ccagcgcggc atcggtgccc gggcgaatag
    34381 gaagccattc atcttcacga ccagcagccg tatcggtata tcgcggatcg ataacaatca
    34441 tttttgcgtt cgatttctcg cgcgcttttt caagaagata agtgatgcca ccgccgctca
    34501 tgcgggtttc agccgggttg ttaccaaaca tcacgaccag cttgctgttt tcaatatccg
    34561 tggtgctgtt gccatcatta ctgccgtagg tgtagggcat ggcacaggaa atttgcgcag
    34621 tgctgtagga gccatactgg tttagtgaac cgccgtagca gttcatcagg cgtttgaccg
    34681 ccgaggctga tggagaagag cgggtcatat tgccgccaac aatccccgaa gagtactgaa
    34741 tatatacagc ctcattgcca tattgttcga cggttttttt caggctactg gcgatagtat
    34801 ccagggcttc atcccagcta atccgttcga atttgccttc gccgcgtttg cccacgcgtt
    34861 tcattgggta attcaggcga tcgggatgat taatacgccg acggatggag cgaccgcgca
    34921 aacaggcgcg tacctgatgg ttgccatact tgtcgctgcc ggtgttgtca gtttccaccc
    34981 aggtcacttc attatcttta acatgcagac gaagtgcaca gcggctacca cagttgacgg
    35041 aacaggcacc ccagaccact ttttcgctgg cctgttgtac cgctgctgct gcactgcgca
    35101 gggtaaacgg caaagaaaaa ccgcctgcag ccagcgccag agaacctatc gcagtagatt
    35161 taacgagtgt tctgcggctg atgcccacca tttgttcatt tttggacata actcactccc
    35221 tgttctttat cgttatataa atgtttatat att
//
LOCUS       DAHHTG010000038        35137 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.038, whole genome shotgun sequence.
ACCESSION   DAHHTG010000038 DAHHTG010000000
VERSION     DAHHTG010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 35137)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35137)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35137
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.038"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            complement(62..793)
                     /gene="rlmB"
                     /locus_tag="NBI74_001842"
     CDS             complement(62..793)
                     /gene="rlmB"
                     /locus_tag="NBI74_001842"
                     /EC_number="2.1.1.185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HCD1932195.1"
                     /translation="MSEMIYGIHAVQALLERAPERFQEVFILKGREDKRLLPLIHALE
                     SQGVVIQLANRQYLDEKSDGAVHQGIIARVKPGRQYQENDLPDLIASLDQPFLLILDG
                     VTDPHNLGACLRSADAAGVHAVIVPKDRSAQLNATAKKVACGAAESVPLIRVTNLART
                     MRMLQEENIWIVGTAGEADHTLYQSKMTGRLALVMGAEGEGMRRLTREHCDELISIPM
                     AGSVSSLNVSVATGICLFEAVRQRS"
     gene            complement(884..3325)
                     /gene="rnr"
                     /locus_tag="NBI74_001843"
     CDS             complement(884..3325)
                     /gene="rnr"
                     /locus_tag="NBI74_001843"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313182.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HCD1932196.1"
                     /translation="MSQDPFQEREAEKYANPIPSREFILEHLTKREKPASRDELAVEL
                     HIEGEEQLEGLRRRLRAMERDGQLVFTRRQCYALPERLDLVKGTVIGHRDGYGFLRVE
                     GRKDDLYLSSEQMKTCIHGDQVLAQPLGADRKGRREARIVRVLVPKTSQIVGRYFTEA
                     GVGFVVPDDSRLSFDILIPPDQIMGARMGFVVVVELTQRPTRRTKAVGKIVEVLGDNM
                     GTGMAVDIALRTHEIPYIWPQAVEQQVAGLKEEVPEEAKAGRVDLRDLPLVTIDGEDA
                     RDFDDAVYCEKKRGGGWRLWVAIADVSYYVRPPTPLDREARNRGTSVYFPSQVIPMLP
                     EVLSNGLCSLNPQVDRLCMVCEMTVSSKGRLTGYKFYEAVMSSHARLTYTKVWHILQG
                     DQDLREQYAPLVKHLEELHNLYKVLDKAREERGGISFESEEAKFIFNAERRIERIEQT
                     QRNDAHKLIEECMILANISAARFVEKAKEPALFRIHDKPSTEAITSFRSVLAELGLEL
                     PGGNKPEPRDYAELLESVADRPDAEMLQTMLLRSMKQAIYDPENRGHFGLALQSYAHF
                     TSPIRRYPDLTLHRAIKYLLAKEQGHQGNTTETGGYHYSMEEMLQLGQHCSMAERRAD
                     EATRDVSDWLKCDFMLDQVGNVFKGVISSVTGFGFFVRLDDLFIDGLVHVSSLDNDYY
                     RFDQVGQRLMGESSGQTYRLGDRVEVRVEAVNMDERKIDFSLISSERAPRNVGKTARE
                     KAKKGDAGKKGGKRRQVGKKVNFEPDSAFRGEKKPKPKAAKKDARKAKKPSAKTQKIA
                     AATKAKRAAKKKVAE"
     gene            complement(3364..3789)
                     /gene="nsrR"
                     /locus_tag="NBI74_001844"
     CDS             complement(3364..3789)
                     /gene="nsrR"
                     /locus_tag="NBI74_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide-sensing transcriptional repressor
                     NsrR"
                     /protein_id="HCD1932197.1"
                     /translation="MQLTSFTDYGLRALIYMASLPEGRMTSISEVTDVYGVSRNHMVK
                     IINQLSRAGYVTAVRGKNGGIRLGKPASAIRIGDVVRELEPLSLVNCSSEFCHITPAC
                     RLKQALSKAVQSFLTELDNYTLADLVEENQPLYKLLLVE"
     gene            complement(3994..5292)
                     /gene="purA"
                     /locus_tag="NBI74_001845"
     CDS             complement(3994..5292)
                     /gene="purA"
                     /locus_tag="NBI74_001845"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HCD1932198.1"
                     /translation="MGNNVVVLGTQWGDEGKGKIVDLLTERAKYVVRYQGGHNAGHTL
                     VINGEKTVLHLIPSGILRENVTSIIGNGVVLSPAALMKEMKELEDRGIPVRERLLLSE
                     ACPLILDYHVALDNAREKARGAKAIGTTGRGIGPAYEDKVARRGLRVGDLFDKETFAE
                     KLKEVMEYHNFQLVNYYKAEAVDYQKVLDDTMAVADILTSMVVDVSDLLDQARQRGDF
                     VMFEGAQGTLLDIDHGTYPYVTSSNTTAGGVATGSGLGPRYVDYVLGILKAYSTRVGA
                     GPFPTELFDETGEFLCKQGNEFGATTGRRRRTGWLDTVAVRRAVQLNSLSGFCLTKLD
                     VLDGLKEVKLCVAYRMPDGREVTTTPLAADDWKGLEPIYETMPGWSESTFGVKDRSGL
                     PQAALNYIKRIEELTGVPIDIISTGPDRTETMILRDPFDA"
     gene            complement(5396..5593)
                     /locus_tag="NBI74_001846"
     CDS             complement(5396..5593)
                     /locus_tag="NBI74_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2065 domain-containing protein"
                     /protein_id="HCD1932199.1"
                     /translation="MNSTIWLALALVLVLEGLGPMLYPKAWKKMISAMTNLPDNILRR
                     FGGGLVVAGVVVYYMLRKTIG"
     gene            complement(5675..6679)
                     /gene="hflC"
                     /locus_tag="NBI74_001847"
     CDS             complement(5675..6679)
                     /gene="hflC"
                     /locus_tag="NBI74_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HCD1932200.1"
                     /translation="MRKSVIAIIIIVLVVLYMSVFVVKEGERGITLRFGKVLRDDDNK
                     PLVYEPGLHFKIPFIETVKMLDARIQTMDNQADRFVTKEKKDLIVDSYIKWRISDFSR
                     YYLATGGGDISQAEVLLKRKFSDRLRSEIGRLDVKDIVTDSRGRLTLEVRDALNSGSA
                     GTEDEVTTPAADNAIAEAAERVTAETKGKVPVINPNSMAALGIEVVDVRIKQINLPTE
                     VSEAIYNRMRAEREAVARRHRSQGQEEAEKLRATADYEVTRTLAEAERQGRIMRGEGD
                     AEAAKLFADAFSKDPDFYAFIRSLRAYENSFSGNQDVMVMSPDSDFFRYMKTPTSATR
                     "
     gene            complement(6682..7941)
                     /gene="hflK"
                     /locus_tag="NBI74_001848"
     CDS             complement(6682..7941)
                     /gene="hflK"
                     /locus_tag="NBI74_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HCD1932201.1"
                     /translation="MAWNQPGNNGQDRDPWGSSKPGGNSEGNGNKGGRDQGPPDLDDI
                     FRKLSKKLGGLGGGKGTGSGGGSSSQGPRPQLGGRVVTIAAAAIVIIWAASGFYTIKE
                     AERGVVTRFGKFSHLVEPGLNWKPTFIDEVKPVNVEAVRELAASGVMLTSDENVVRVE
                     MNVQYRVTDPEKYLYSVTSPDDSLRQATDSALRGVIGKYTMDRILTEGRTVIRSDTQR
                     ELEETIRPYDMGITLLDVNFQAARPPEEVKAAFDDAIAARENEQQYIREAEAYTNEVQ
                     PRANGQAQRILEEARAYKAQTILEAQGEVARFAKLLPEYKAAPEITRERLYIETMEKV
                     LGNTRKVLVNDKGGNLMVLPLDQMLKGGNAPAAKSDNGASNLLRLPPASSSTTSGASN
                     TSSTSQGDIMDQRRANAQRNDYQRQGE"
     gene            complement(8027..9307)
                     /gene="hflX"
                     /locus_tag="NBI74_001849"
     CDS             complement(8027..9307)
                     /gene="hflX"
                     /locus_tag="NBI74_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HCD1932202.1"
                     /translation="MFDRYDAGEQAVLVHIYFTQDKDMEDLQEFESLVSSAGVEALQV
                     ITGSRKAPHPKYFVGEGKAVEIAEAVKATGASVVLFDHALSPAQERNLERLCECRVID
                     RTGLILDIFAQRARTHEGKLQVELAQLRHLATRLVRGWTHLERQKGGIGLRGPGETQL
                     ETDRRLLRNRIVQIQSRLERVEKQREQGRQSRIKADVPTVSLVGYTNAGKSTLFNRIT
                     EARVYAADQLFATLDPTLRRIDVADVGETVLADTVGFIRHLPHDLVAAFKATLQETRQ
                     ATLLLHVIDAADVRVQENIEAVNTVLEEIDAHEIPTLLVMNKIDMLDDFEPRIDRDEE
                     NKPIRVWLSAQTGAGIPQLFQALTERLSGEVAQHTLRLPPQEGRLRSRFYQLQAIEKE
                     WMEEDGSVSLQVRMPIVDWRRLCKQEPALIDYLI"
     gene            complement(9383..9691)
                     /gene="hfq"
                     /locus_tag="NBI74_001850"
     CDS             complement(9383..9691)
                     /gene="hfq"
                     /locus_tag="NBI74_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HCD1932203.1"
                     /translation="MAKGQSLQDPFLNALRRERVPVSIYLVNGIKLQGQIESFDQFVI
                     LLKNTVSQMVYKHAISTVVPSRPVSHHSNNAGGGTSSNYHHGSSAQNTSAQQDSEETE
                     "
     gene            complement(9777..10727)
                     /gene="miaA"
                     /locus_tag="NBI74_001851"
     CDS             complement(9777..10727)
                     /gene="miaA"
                     /locus_tag="NBI74_001851"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HCD1932204.1"
                     /translation="MSDISKASLPKAIFLMGPTASGKTALAIELRKILPVELISVDSA
                     LIYKGMDIGTAKPNAEELLAAPHRLLNIRDPSQAYSAADFRRDALAEMADITAAGRIP
                     LLVGGTMLYFKALLEGLSPLPSADPEVRARIEQQAAEQGWESLHRQLQEVDPVAAARI
                     HPNDPQRLSRALEVFFISGKTLTELTQTSGDALPYQVHQFAIAPASRELLHQRIEQRF
                     HQMLASGFEAEVRALFARGDLHTDLPSIRCVGYRQMWSYLEGEISYDEMVYRGVCATR
                     QLAKRQITWLRGWEGVHWLDSEKPEQARDEVLQVVGAIAG"
     gene            complement(10720..12567)
                     /gene="mutL"
                     /locus_tag="NBI74_001852"
     CDS             complement(10720..12567)
                     /gene="mutL"
                     /locus_tag="NBI74_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HCD1932205.1"
                     /translation="MPIQVLPPQLANQIAAGEVVERPASVVKELVENSLDAGATRIDI
                     DIERGGAKLIRIRDNGCGIKKDELALALARHATSKIASLDDLEAIISLGFRGEALASI
                     SSVSRLTLTSRTAEQQEAWQAYAEGRNMDVTVKPAAHPVGTTLEVLDLFYNTPARRKF
                     LRTEKTEFNHIDEIIRRIALARFDVTINLSHNGKIVRQYRAVPEGGQKERRLGAICGT
                     AFLEQALAIEWQHGDLTLRGWVADPNHTTPALAEIQYCYVNGRMMRDRLINHAIRQAC
                     EDKLGADQQPAFVLYLEIDPHQVDVNVHPAKHEVRFHQSRLVHDFIYQGVLSVLQQQL
                     ETPLPLDDEPQPAPRAIPENRVAAGRNHFAEPAVREPVAPRYTPAPSSGSRPAAPWPN
                     AQPGYQKQQGEVYRQLLQTPAPMQKPKAPEPQEPALAANSQSFGRVLTIVHSDCALLE
                     RDGNISLLSLPVAERWLRQAQLTPGEVPVCAQPLLIPLRLKVSGEEKSALEKAQSALA
                     ELGIDFQSDAQHVTIRAVPLPLRQQNLQILIPELIGYLAKQSVFEPGNIAQWIARNLM
                     SEHAQWSMAQAITLLADVERLCPQLVKTPPGGLLQSVDLHPAIKALKDE"
     gene            complement(12577..13908)
                     /gene="amiB"
                     /locus_tag="NBI74_001853"
     CDS             complement(12577..13908)
                     /gene="amiB"
                     /locus_tag="NBI74_001853"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiB"
                     /protein_id="HCD1932206.1"
                     /translation="MMYRIRNWLVATLLLLCAQAGAATLSDIQVSNGNQQARITLSFI
                     GDPDYAFSHQSKRTVALDIKQTGVIQGLPLLFSGNNLVKAIRSGTPKDAQTLRLVVDL
                     TENGKTEAVKRQNGSNYTVVFTINADAPPSPPPMVAKRVETPAVVAPRVSEPARNPFK
                     TESNRTTGVISSNTVTRPAARATANTGDKIIIAIDAGHGGQDPGAIGPGGTREKNVTI
                     AIARKLRTLLNNDPMFKGVLTRDGDYFISVMGRSDVARKQNANFLVSIHADAAPNRSA
                     TGASVWVLSNRRANSEMASWLEQHEKQSELLGGAGDVLANSQADPYLSQAVLDLQFGH
                     SQRVGYDVATSMISQLQRIGEIHKRRPEHASLGVLRSPDIPSVLVETGFISNNSEERL
                     LASDDYQQQLAEAIYKGLRNYFLAHPMQSAPQGATAQTASTVTTPDRTLPN"
     gene            complement(13927..14388)
                     /gene="tsaE"
                     /locus_tag="NBI74_001854"
     CDS             complement(13927..14388)
                     /gene="tsaE"
                     /locus_tag="NBI74_001854"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HCD1932207.1"
                     /translation="MMNRVIPLPDEQATLDLGERVAKACDGATVIYLYGDLGAGKTTF
                     SRGFLQALGHQGNVKSPTYTLVEPYTLDNLMVYHFDLYRLADPEELEFMGIRDYFAND
                     AICLVEWPQQGTGVLPDPDVEIHIDYQAQGREARVSAVSSAGELLLARLAG"
     gene            complement(14360..15907)
                     /gene="nnr"
                     /locus_tag="NBI74_001855"
     CDS             complement(14360..15907)
                     /gene="nnr"
                     /locus_tag="NBI74_001855"
                     /EC_number="4.2.1.136"
                     /EC_number="5.1.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ADP-dependent NAD(P)H-hydrate
                     dehydratase/NAD(P)H-hydrate epimerase"
                     /protein_id="HCD1932208.1"
                     /translation="MTDHTMKKNPVSIPHTVWYADDIRRGEREAADALGLTLYELMLR
                     AGEAAFQVCRSAYPDARHWLVLCGHGNNGGDGYVVARLAKAIGIDVTLLAQESDKPLP
                     EEAALAREAWLNAGGEIHASNIVWPESVDLIVDALLGTGLQQAPRESISQLIDHANSH
                     PAPIVAVDIPSGLLAETGATPGAVINADHTITFIALKPGLLTGKARDVTGQLHFDSLR
                     LDRWLAGQETKIQRFSAEQLSQWLIPRRPTSHKGDHGRLVIIGGDHGTAGAIRMTGEA
                     ALRAGAGLVRVLTRSENIAPLLTARPELMVHELTMGSLTESLEWADVVVIGPGLGQQE
                     WGKKALQKVENFRKPMLWDADALNLLAINPDKRHNRVITPHPGEAARLLGCSVAEIES
                     DRLHCAQRLVQRYGGVAVLKGAGTVVAAHPDALGIIDAGNAGMASGGMGDVLSGIIGA
                     LLGQKMSPYDAACAGCVAHGAAADVLAARFGTRGMLATDLFSTLQRIVNPEVTDKNHD
                     ESSNSAP"
     gene            15906..17045
                     /gene="queG"
                     /locus_tag="NBI74_001856"
     CDS             15906..17045
                     /gene="queG"
                     /locus_tag="NBI74_001856"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HCD1932209.1"
                     /translation="MSEPLDLNQLAQKIKQWGLELGFQQVGITDTDLSESEPKLQAWL
                     DKQYHGEMDWMARHGMLRARPHELLPGTLRVISVRMNYLPANAAFASTLKNPKLGYVS
                     RYALGRDYHKLLRNRLKKLGEMIQQHCVSLNFRPFVDSAPILERPLAEKAGLGWTGKH
                     SLILNREAGSFFFLGELLVDIPLPVDQPVEEGCGKCVACMTICPTGAIVEPYTVDARR
                     CISYLTIELEGEIPEELRPLMGNRIYGCDDCQLICPWNRYSQLTTEDDFSPRKPLHAP
                     ELIELFAWSEEKFLKVTEGSAIRRIGHLRWLRNIAVALGNAPWDETILTALESRKGEH
                     PLLDEHIAWAIAQQIERRNACVVEVQLPKKQRLVRVIEKGLPRDA"
     gene            complement(17028..17081)
                     /locus_tag="NBI74_001857"
     CDS             complement(17028..17081)
                     /locus_tag="NBI74_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1932210.1"
                     /translation="MRFYFYSQAVDESGVTR"
     gene            complement(17315..17390)
                     /locus_tag="NBI74_001858"
     tRNA            complement(17315..17390)
                     /locus_tag="NBI74_001858"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17355..17357),aa:Gly,seq:gcc)
     gene            complement(17426..17501)
                     /locus_tag="NBI74_001859"
     tRNA            complement(17426..17501)
                     /locus_tag="NBI74_001859"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17466..17468),aa:Gly,seq:gcc)
     gene            complement(17538..17613)
                     /locus_tag="NBI74_001860"
     tRNA            complement(17538..17613)
                     /locus_tag="NBI74_001860"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17578..17580),aa:Gly,seq:gcc)
     gene            complement(17824..18369)
                     /gene="orn"
                     /locus_tag="NBI74_001861"
     CDS             complement(17824..18369)
                     /gene="orn"
                     /locus_tag="NBI74_001861"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HCD1932211.1"
                     /translation="MSANENNLIWIDLEMTGLDPERDRIIEIATLVTDANLNILAEGP
                     TIAVHQSDEQLALMDDWNVRTHTASGLVERVKASTMGDREAELATLEFLKQWVPAGKS
                     PICGNSIGQDRRFLFKYMPELEAYFHYRYLDVSTLKELARRWKPEILDGFTKQGTHQA
                     MDDIRESVAELAYYREHFIKL"
     gene            18464..19516
                     /gene="rsgA"
                     /locus_tag="NBI74_001862"
     CDS             18464..19516
                     /gene="rsgA"
                     /locus_tag="NBI74_001862"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small ribosomal subunit biogenesis GTPase RsgA"
                     /protein_id="HCD1932212.1"
                     /translation="MSKNKLSKGQQRRVNANHQRRLKTSKEKPDYDDNLFGEPDEGIV
                     ISRFGMHADVESADGDVHRCNIRRTIRSLVTGDRVVWRPGKPAAEGVNVKGIVEAVHE
                     RTSVLTRPDFYDGVKPIAANIDQIVIVSAILPELSLNIIDRYLVACETLQIEPIIVLN
                     KIDLLDDEGMEFVNEQMDIYRNIGYRVLMVSSHTQDGLKPLEEALTGRISIFAGQSGV
                     GKSSLLNALLGLQKEILTNDVSDNSGLGQHTTTAARLYHFPHGGDVIDSPGVREFGLW
                     HLEPEQITQGFVEFHDYLGLCKYRDCKHDTDPGCAIREAVEEGKIAETRFENYHRILE
                     SMAQVKTRKNFSDTDD"
     gene            19613..20581
                     /gene="psd"
                     /locus_tag="NBI74_001863"
     CDS             19613..20581
                     /gene="psd"
                     /locus_tag="NBI74_001863"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313166.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HCD1932213.1"
                     /translation="MLNSFKLSLQYILPKLWLTRLAGWGASKRAGWLTKLVIDLFVKY
                     YKVDMKEAQKPDTASYRTFNEFFVRPLRDEVRPIDTDPNVLVMPADGVISQLGKIEED
                     KILQAKGHNYSLEALLAGNYLMADLFRNGTFVTTYLSPRDYHRVHMPCNGILREMIYV
                     PGDLFSVNHLTAQNVPNLFARNERVICLFDTEFGPMAQILVGATIVGSIETVWAGTIT
                     PPREGIIKRWTWPAGENDGSVALLKGQEMGRFKLGSTVINLFAPGKVDLVEQLESLSV
                     TKIGQPLAVSTETFVTPDAEPAPLPAEEIEAEHDASPLVDDKKDQV"
     gene            20603..23926
                     /gene="mscM"
                     /locus_tag="NBI74_001864"
     CDS             20603..23926
                     /gene="mscM"
                     /locus_tag="NBI74_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="miniconductance mechanosensitive channel MscM"
                     /protein_id="HCD1932214.1"
                     /translation="MRLIITFLMAWCLSWGAYAATAPDSKQITQELEQAKAAKPAQPE
                     VVEALQSALNALEERKGSLERIKQYQEVIDNYPKLSATLRAQLNNMRDEPRSVSPGMS
                     NDALNQEILQISSQLLDKSRQAQQEQERAREIADSLNQLPQQQTDARRQLNEIERRLG
                     TLTGNTPLNQAQNFALQSDSARLKALVDELELAQLSANNRQELARLRSELAEKESQQL
                     DAYLQALRNQLNSQRQLEAERALESTEQLAESSADLPKDIVAQFKINRELSAALNQQA
                     QRMDLVASQQRQAASQTLQVRQALNTLREQSQWLGSSNLLGEALRAQVARLPEMPKPQ
                     QLDTEMAQLRVQRLRYEDLLNKQPLLRQIHQADGQPLTAEQNRILEAQLRTQRELLNS
                     LLQGGDTLLLELTKLKVSNGQLEDALKEVNEATHRYLFWTSDVRPMTIAWPLEIAQDL
                     RRLISLDTFSQLGKASVMMLTSKETILPLFGALILVGCSIYSRRYFTRFLERSAAKVG
                     KVTQDHFWLTLRTLFWSILVASPLPVLWMTLGYGLREAWPYPLAVAIGDGVTATVPLL
                     WVVMICATFARPNGLFIAHFGWPRERVSRGMRYYLMSIGLIVPLIMALMMFDNLDDRE
                     FSGSLGRLCFILICGALAVVTLSLKKAGIPLYLNKEGSGDNITNHMLWNMMIGAPLVA
                     ILASAVGYLATAQALLARLETSVAIWFLLLVVYHVIRRWMLIQRRRLAFDRAKHRRAE
                     MLAQRARGEEEAHHHSSPEGAIEVDESEVDLDAISAQSLRLVRSILMLIALLSVIVLW
                     SEIHSAFGFLENISLWDVTSTVQGVESLEPITLGAVLIAILVFIITTQLVRNLPALLE
                     LAILQHLDLTPGTGYAITTITKYLLMLIGGLVGFSMIGIEWSKLQWLVAALGVGLGFG
                     LQEIFANFISGLIILFEKPIRIGDTVTIRDLTGSVTKINTRATTISDWDRKEIIVPNK
                     AFITEQFINWSLSDSVTRVVLTIPAPANANSEEVTEILLTAARRCSLVIDNPAPEVFL
                     VDLQQGIQIFELRIYAAEMGHRMPLRHEIHQLILAGFHAHGIDMPFPPFQMRLESLNG
                     KQTGRTLTSAGKGRQAGSL"
     gene            complement(23957..24271)
                     /locus_tag="NBI74_001865"
     CDS             complement(23957..24271)
                     /locus_tag="NBI74_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjeO family protein"
                     /protein_id="HCD1932215.1"
                     /translation="MSARMSVLCCIWFIVAFLWITITSALDKEWMIDGRGINNVCDVL
                     MYLEDDDTRDVGVIMTLPLFFPFLWFALWRKKRGWFMYATALAIFGYWLWQFFLRYQF
                     CL"
     gene            complement(24369..25871)
                     /gene="yjeM"
                     /locus_tag="NBI74_001866"
     CDS             complement(24369..25871)
                     /gene="yjeM"
                     /locus_tag="NBI74_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418580.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/gamma-aminobutyrate family transporter
                     YjeM"
                     /protein_id="HCD1932216.1"
                     /translation="MPHSIKKMSLIGLILMIFTSVFGFANSPSAYYLMGYSAISFYIF
                     SALLFFIPFALMMAEMGAAYRKEEGGIYSWMNNSVGPRFAFIGTFMWFSSYIIWMVST
                     SAKVWVPFSTFLYGSDMTQHWHIAGLEPTQVVGLLAVAWMILVTVVASKGINKIARIT
                     AVGGIAVMCLNLVLLLVSITILLLNGGHFAQDINFLASPNPGYQSGLAMLSFVVFAIF
                     AYGGIEAVGGLVDKTENPEKNFAKGIVFAAIVISIGYSLAIFLWGVSTNWQQVLSNGS
                     VNLGNITYVLMKSLGVTLGNALHLSPEASLSLGVWFARITGLSMFLAYTGAFFTLCYS
                     PLKAIIQGTPKALWPEPMTRLNAMGMPSIAMWMQCGLVTVFILLVSFGGGTASAFFNK
                     LTLMANVSMTLPYLFLALAFPFFKARQDLDRPFVIFKTRMSAMIATVVVVLVVTFANV
                     FTIIQPVVEAGDWDSTLWMIGGPVFFSLLAMAIYQNYCSRVVKNPQWVAE"
     gene            complement(26090..27067)
                     /gene="epmA"
                     /locus_tag="NBI74_001867"
     CDS             complement(26090..27067)
                     /gene="epmA"
                     /locus_tag="NBI74_001867"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P--(R)-beta-lysine ligase"
                     /protein_id="HCD1932217.1"
                     /translation="MSETASWQPSASIPNLLKRAAIMAEIRRFFADRGVLEVETPCMS
                     QATVTDIHLVPFETRFVGPGHSQGMNLWLMTSPEYHMKRLLVAGCGPVFQLCRSFRNE
                     EMGRYHNPEFTMLEWYRPHYDMYRLMNEVDDLLQQVLDCPAAESLSYQQAFLRYLEID
                     PLSADKTQLREVAAKLDLSNVADTEEDRDTLLQLLFTFGVEPNIGKEKPTFVYHFPAS
                     QASLAQISTEDHRVAERFEVYYKGIELANGFHELTDAREQQQRFEQDNRKRAARGLPQ
                     HPIDQNLIEALKVGMPDCSGVALGVDRLVMLALGAETLAEVIAFSVDRA"
     gene            27392..29200
                     /gene="frdA"
                     /locus_tag="NBI74_001868"
     CDS             27392..29200
                     /gene="frdA"
                     /locus_tag="NBI74_001868"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HCD1932218.1"
                     /translation="MQTFQADLAIVGAGGAGLRAAIAAAQANPNAKIALISKVYPMRS
                     HTVAAEGGSAAVAQDHDSFEYHFHDTVAGGDWLCEQDVVDYFVHHCPTEMTQLELWGC
                     PWSRRPDGSVNVRRFGGMKIERTWFAADKTGFHMLHTLFQTSLQFPQIQRFDEHFVLD
                     ILVDDGHVRGLVAMNMMEGTLVQIRANAVVMATGGAGRVYRYNTNGGIVTGDGMGMAL
                     SHGVPLRDMEFVQYHPTGLPGSGILMTEGCRGEGGILVNKNGYRYLQDYGMGPETPLG
                     EPKNKYMELGPRDKVSQAFWHEWRKGNTISTPRGDVVYLDLRHLGEKKLHERLPFICE
                     LAKAYVGVDPVKEPIPVRPTAHYTMGGIETDQNCETRIKGLFAVGECSSVGLHGANRL
                     GSNSLAELVVFGRLAGEQATERAATAGNGNEAAIEAQAAGVEQRLKDLVNQDGGENWA
                     KIRDEMGMAMEEGCGIYRTPELMQKTIDKLAELQERFKRVRITDTSSVFNTDLLYTIE
                     LGHGLNVAECMAHSAMARKESRGAHQRLDEGCTERDDVNFLKHTLAFRDADGTTRLEY
                     SDVKITTLPPAKRVYGGEADAADKAEAANKKEKANG"
     gene            29193..29927
                     /gene="frdB"
                     /locus_tag="NBI74_001869"
     CDS             29193..29927
                     /gene="frdB"
                     /locus_tag="NBI74_001869"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur protein"
                     /protein_id="HCD1932219.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALGYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            29938..30333
                     /gene="frdC"
                     /locus_tag="NBI74_001870"
     CDS             29938..30333
                     /gene="frdC"
                     /locus_tag="NBI74_001870"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HCD1932220.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FGLFALKNGPEAWAGFVDFLQNPVIVIINLITLAAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            30344..30703
                     /gene="frdD"
                     /locus_tag="NBI74_001871"
     CDS             30344..30703
                     /gene="frdD"
                     /locus_tag="NBI74_001871"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HCD1932221.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGVVTI"
     gene            30766..31899
                     /locus_tag="NBI74_001872"
     CDS             30766..31899
                     /locus_tag="NBI74_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HCD1932222.1"
                     /translation="MFKTTLCTLLITASCSTFAAPQQINDIVHRTITPLIEQQRIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPTTKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMKTRVFQPLKLNHTWINVPSAEEKNYAWG
                     YREGKAVHVSPGALDAEAYGVKSTIEDMARWVQSNLKPLDINEKILQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDIIINGSDNKIALAARPVKPITPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVTAAWQILNTLQ"
     gene            31988..32521
                     /gene="blc"
                     /locus_tag="NBI74_001873"
     CDS             31988..32521
                     /gene="blc"
                     /locus_tag="NBI74_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HCD1932223.1"
                     /translation="MRLLPLVAAATAAFLVVACSSPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDREMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(32518..32835)
                     /gene="sugE"
                     /locus_tag="NBI74_001874"
     CDS             complement(32518..32835)
                     /gene="sugE"
                     /locus_tag="NBI74_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HCD1932224.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSMA
                     LLAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
     gene            complement(33011..33157)
                     /gene="ecnB"
                     /locus_tag="NBI74_001875"
     CDS             complement(33011..33157)
                     /gene="ecnB"
                     /locus_tag="NBI74_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HCD1932225.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            complement(33268..33393)
                     /gene="ecnA"
                     /locus_tag="NBI74_001876"
     CDS             complement(33268..33393)
                     /gene="ecnA"
                     /locus_tag="NBI74_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HCD1932226.1"
                     /translation="MMKRLIVLVLLASTLLTGCNTARGFGEDIKHLGNSISRAAS"
     gene            complement(33445..34011)
                     /gene="efp"
                     /locus_tag="NBI74_001877"
     CDS             complement(33445..34011)
                     /gene="efp"
                     /locus_tag="NBI74_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HCD1932227.1"
                     /translation="MATYYSNDFRAGLKIMLDGEPYAVEASEFVKPGKGQAFARVKLR
                     RLLTGTRVEKTFKSTDSAEGADVVDMNLTYLYNDGEFWHFMNNETFEQLSADAKAIGD
                     NAKWLLDQAECIVTLWNGQPISVTPPNFVELEIVDTDPGLKGDTAGTGGKPATLSTGA
                     VVKVPLFVQIGEVIKVDTRSGEYVSRVK"
     gene            34053..35081
                     /gene="epmB"
                     /locus_tag="NBI74_001878"
     CDS             34053..35081
                     /gene="epmB"
                     /locus_tag="NBI74_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HCD1932228.1"
                     /translation="MAHIVTLNTPSREDWLTQLADVVTDPDELLRLLNIDADEKLLAG
                     RSAKKLFALRVPRSFIDRMEKGNPNDPLLRQVITSQDEFVVAPGFSTDPLEEQHSVVP
                     GLLHKYHNRALLLVKGGCAVNCRYCFRRHFPYAENQGNKRNWQTALEYVAAHPELDEM
                     IFSGGDPLMAKDHELDWLLTQLEAIPHIKRLRIHSRLPIVIPARITDALVERFSHSTL
                     QILLVNHINHANEVDETFRQAMAKLRRVGVTLLNQSVLLRGVNDNAQTLANLSNALFD
                     AGVMPYYLHVLDKVQGAAHFMVSDDEARQIMRELLTLVSGYLVPKLAREIGGEPSKTP
                     LDLQLRQQ"
BASE COUNT         8394 a         9630 c         8913 g         8200 t
ORIGIN      
        1 taccagctat agacaaaaaa aaaccatcca aatctggatg gcttttcata attcagagga
       61 actagctgcg ctgacgaacc gcttcaaata agcaaattcc ggttgcaacc gaaacgttca
      121 gggaagaaac acttcctgcc attgggatgc tgatcaactc atcgcaatgt tcacgggtca
      181 ggcgacgcat accttcacct tccgcgccca tcaccagcgc caggcgtccg gtcattttgc
      241 tctgatagag tgtatgatcc gcctcgcctg ccgtaccgac gatccagata ttctcttcct
      301 gcaacatacg catggtgcgc gccagattag tcacccgaat cagcggaacg ctttctgccg
      361 caccacaggc cacttttttc gccgtggcgt tgagctgtgc ggagcgatct ttcggcacaa
      421 tcaccgcatg aacaccagcg gcgtccgcgc tacgcaggca cgcgccgagg ttgtgcggat
      481 cggttacgcc gtcgaggatc agcaggaacg gttgatcgag cgaagcgatc agatccggca
      541 gatcgttttc ctgatactgc cgtcctggct tcacacgagc gataatgccc tgatgcacgg
      601 caccgtcgct tttctcgtcg agatattggc ggtttgccaa ctggataacc acgccctgag
      661 actcaagggc gtgaatcagc ggtaacagac gtttatcttc acggcctttt aaaataaaga
      721 cttcctgaaa acgttcaggg gcgcgctcca gcagggcctg cactgcatgg atgccgtaaa
      781 tcatttcgct cattaatgta ctcgttgttt acgttttgcc gggagcgcca cgcttgcccg
      841 gcctacggat aattcaccct cttctgtttg aagagggtaa tgatcactct gccacttttt
      901 tcttcgccgc acgcttcgct ttggtcgctg cggctatttt ctgcgttttc gccgatggct
      961 ttttcgcttt tctcgcgtct ttcttcgctg ctttcggctt cggttttttc tcgccacgga
     1021 aggcgctgtc tggctcaaag ttgacctttt tgccgacctg acgccgcttg ccgccttttt
     1081 tgcctgcatc gccttttttc gctttctcgc gcgccgtttt accgacgttg cgcggtgcgc
     1141 gttcgctgga gatcaggcta aagtcgattt tgcgctcgtc catattaacc gcttcgacgc
     1201 gaacttccac gcgatcgccc aggcgataag tctgaccgct ggattcaccc atcaggcgtt
     1261 gccctacctg gtcaaagcga tagtagtcat tgtccagcga agagacatgg accagaccat
     1321 caatgaacaa gtcgtccaga cggacgaaga agccaaagcc agtgacgctg gaaattacgc
     1381 ctttaaagac gttacctacc tggtcgagca tgaagtcaca cttcagccag tcagacacat
     1441 cgcgcgttgc ttcatcggca cgacgttccg ccatcgaaca gtgctgaccc agttgcagca
     1501 tctcttccat cgaataatgg tagccgccgg tttcagtggt gttgccctga tgcccctgct
     1561 ctttcgccag cagatattta atggcgcggt gcaacgtcag gtcaggataa cgacgaatcg
     1621 gcgaagtaaa gtgcgcatag gactgcaatg ccagaccgaa gtgaccacgg ttttccggat
     1681 cgtaaatcgc ctgtttcatc gagcgcaaaa gcatggtttg cagcatttct gcatcaggac
     1741 gatcggcaac cgattccagt aactctgcat agtcgcgcgg ttccggcttg ttaccgcccg
     1801 gcaattccag ccccagctcc gccagcactg aacggaaaga ggtaatcgct tcggtgctcg
     1861 gcttgtcgtg aatacggaac agtgccggtt ctttggcttt ctcaacgaaa cgcgccgccg
     1921 agatattcgc cagaatcatg cactcttcaa ttaacttgtg ggcgtcgtta cgctgagtct
     1981 gttcgatacg ttcaatacgg cgttcagcgt tgaaaatgaa cttcgcttct tcgctctcaa
     2041 atgagatccc accgcgttct tcacgggctt tatccagcac tttatagagg ttatgtaact
     2101 cttcgagatg cttaaccagc ggcgcatact gctcgcgcag atcctgatcg ccttgcagta
     2161 tatgccagac tttggtgtag gtcagacgcg cgtgagaact catcaccgct tcgtagaatt
     2221 tgtagccagt caggcggcct ttcgacgaaa ccgtcatctc gcacaccata cacaggcggt
     2281 ctacctgcgg gttgagcgag cacaggccgt tagagagcac ttccggcagc atcgggataa
     2341 cctgcgaagg gaagtacacc gacgtaccac ggttacgcgc ttctctgtcc agcggtgttg
     2401 gcggacggac atagtaactg acgtcggcaa ttgcgaccca caaacgccag ccaccgccgc
     2461 gttttttctc acagtaaact gcatcgtcaa agtcacgggc gtcttcgcca tcaatggtga
     2521 ccagcggtaa atcgcgcaga tcgacacggc ccgcttttgc ttcttccggc acttcttctt
     2581 tcagcccggc aacctgttgc tcaacagcct gcggccagat gtacggaatt tcatgggtac
     2641 gcagagcaat atcaaccgcc atgccggtgc ccatattatc gcccagcact tcgacgattt
     2701 tacccaccgc tttggtacgg cgagtcgggc gttgagtcag ttcaaccacc accacaaagc
     2761 ccatccgcgc gcccatgatc tgatcgggcg ggattaagat atcgaagctc agacggctat
     2821 cgtcaggaac cacaaagccg acgcccgctt cggtgaagta gcgaccaaca atctggctgg
     2881 tttttggcac cagtacgcgg acaatacgcg cttcacgacg acctttacgg tcagcgccca
     2941 gcggctgagc cagcacctga tcgccatgaa tgcaggtttt catctgctcg ctggagagat
     3001 acaaatcatc tttacgccct tcaactcgca gaaagccgta gccatcacgg tggccaataa
     3061 cggtaccttt caccaggtcg aggcgttccg gcagcgcata gcactgacgg cgagtgaaga
     3121 ccagttgacc atcgcgctcc atcgcgcgca ggcgtcgacg caggccttca agctgctctt
     3181 cgccttcaat gtgcagttct accgccagct catcacggct ggccggtttt tcacgtttgg
     3241 ttaaatgttc gaggataaat tcccgactag ggatgggatt cgcgtatttt tcagcttcgc
     3301 gttcctggaa aggatcttgt gacatctcgg ttcctccgtt gtcatctctg atgaagattt
     3361 tcgtcactcc accagcaata atttataaag cggttgattc tcttcaacca aatcggcaag
     3421 cgtgtagtta tccagttccg tcagaaaact ttgcacggcc ttagaaagcg cctgtttcaa
     3481 ccgacaggca ggtgtaatgt ggcaaaactc actgctgcaa ttcaccagcg ataagggctc
     3541 cagctcgcgc accacatcac caatacgtat cgcactcgcc ggtttaccca agcgaatgcc
     3601 gccatttttt ccacgaacag cagtcacgta gccggcacga ctaagttgat tgattatttt
     3661 gaccatatga ttacgggaga cgccgtagac gtcagtcact tcagaaatac tggtcatccg
     3721 cccttctggc aatgacgcca tgtagatcag cgcacgtaat ccgtaatcag tgaaactcgt
     3781 taactgcaca tcaacctcaa aagggaaatc ggggaaaaat acatttatat tgatgataaa
     3841 ccagccacaa gctgtgtcgc taatttattt cagttcgggg aaggaaaaaa acgaggattt
     3901 aacactgtgt cggatagcgt aatggcttaa ccgacacagc aatgtatcac actgttagcc
     3961 cggcaggcaa aatatctgcc atgcgtacca gaattacgcg tcgaacgggt cgcgcagaat
     4021 catggtttca gtacgatccg gaccggtaga gatgatatcg atcggcacac cggtcagctc
     4081 ttcaatgcgc ttgatgtagt tcagtgccgc ctgcggcagg ccactacgat ctttcacgcc
     4141 gaaggtggat tcagaccagc ccggcatggt ttcgtaaatc ggctctagac ctttccagtc
     4201 gtcagctgcc agcggagtgg tagtcacttc acgaccatcc ggcatacggt aagccacgca
     4261 gagtttaacc tctttcaggc catccagaac gtccagttta gtcaggcaga agccagacag
     4321 ggagttcaac tgtaccgcac gacgaacggc aacggtgtcc agccagccgg tacgacgacg
     4381 acgacccgta gttgcgccga attcgttacc ctgcttgcag aggaactcgc cagtttcatc
     4441 aaacagttcg gtcgggaacg gacctgcccc cacacgagtg gagtaagctt tgaggatacc
     4501 cagaacgtaa tcaacataac gcgggcccag gccggaaccg gtcgccacgc caccagcagt
     4561 ggtgttggaa gaagttacgt acggatatgt accgtggtcg atatccagca gcgtaccctg
     4621 cgcaccttca aacatgacga aatcgccacg ctgacgcgcc tggtcgagca ggtcagaaac
     4681 gtcaacaacc atagaagtca ggatgtcggc aacagccatc gtatcatcca gaactttctg
     4741 gtaatcaacc gcttcagctt tgtagtagtt aaccaactgg aagttgtgat attccatcac
     4801 ttctttcagt ttttcagcga aggtttcttt gtcgaaaagg tcgccaacac gcagaccgcg
     4861 acgtgctact ttatcttcat aagcaggccc gataccacga ccggtggtgc cgatcgcttt
     4921 cgcgccacgc gctttctcac gcgcgttatc cagcgcaacg tgataatcaa ggatcagcgg
     4981 acatgcttcg gacagcagca gacgctcacg aacggggatg ccacggtctt ccagttcttt
     5041 catctctttc atcagcgcag ccggagacag cacaacaccg ttaccgatga tgctggttac
     5101 attctcgcgg agaatacctg atggaataag atggagaacg gttttttcac cgttgattac
     5161 gagagtatgg cctgcgttgt gaccgccctg gtagcgtaca acatatttag cccgttcagt
     5221 cagaagatcg acgatcttac ctttaccttc gtcaccccat tgggtgccca gtacgacgac
     5281 gttgttaccc atttttcaaa atcaccgttt gcttaaaaat ggattctacc atcgcttttt
     5341 cagagttaca gcactttttg cactcaaaaa tgacctcgat cagtctgttt tttgttcagc
     5401 caatcgtttt cctcaacatg tagtagacca caacgcccgc aaccacaagt ccaccgccaa
     5461 aacgacgtaa aatattatcg ggcaaattgg tcatcgcaga gatcatcttc ttccatgcct
     5521 tcgggtaaag catcggccct aaaccttcca gtaccaaaac cagggcaagc gccagccaga
     5581 ttgtcgaatt cattcattat ccttatagaa aaagaaaacc accgacatcc ctgaggatgc
     5641 ggtggcttta ttgacctgta ccgcagtcgt tatattaacg cgttgcggaa gtcggcgtct
     5701 tcatgtagcg gaagaaatcg ctgtccgggc tcatgaccat cacgtcctga ttgccagaga
     5761 agctgttctc ataagcacgc aggctacgga tgaatgcgta gaagtccgga tctttactga
     5821 atgcatcagc aaacagtttg gctgcttctg catcgccttc accacgcatg atgcggccct
     5881 gacgctcagc ttctgccagc gttctggtca cttcatagtc ggcagtcgcg cgcagttttt
     5941 ccgcttcttc ctgaccttgt gaacggtgac gacgtgctac cgcttcacgc tcggcgcgca
     6001 tacggttgta gatcgcttca gacacttcgg tcggcaggtt gatctgctta attcgcacat
     6061 cgacaacttc aatacccagc gccgccatac tgttcgggtt gatgaccgga actttgccct
     6121 tcgtctcagc cgttacgcgc tctgccgctt cggcaatggc gttatctgct gcaggggtag
     6181 taacttcatc ttctgtaccc gcagaaccgg agttcagcgc gtcacgtact tcgagggtca
     6241 gacgaccacg ggaatcggtg acgatatctt tcacgtccag gcgaccaatt tcagaacgca
     6301 gacggtcaga gaacttacgt ttcaacagca cttccgcttg cgaaatgtcg ccaccacccg
     6361 ttgccagata gtaacggctg aaatcgctga tgcgccattt gatgtaagag tcgacgatca
     6421 ggtctttctt ctctttggtc acaaagcggt cggcctggtt gtccatggtc tgaatacgtg
     6481 cgtcgagcat tttcaccgtt tcaatgaacg gtatcttgaa atgcagaccc ggctcataaa
     6541 ccagaggttt gttgtcatcg tcacgcagta ccttaccaaa acgcagcgta ataccgcgct
     6601 caccttcttt gacgacaaag acagacatgt aaagcactac cagcacgatg atgataatcg
     6661 cgataactga cttacgcatc gttattcccc ctgacgctgg tagtcgttac gctgcgcgtt
     6721 ggcgcggcgt tggtccataa tatcgccctg actggtggac gacgtgttgc ttgctccact
     6781 ggttgtggag gaagaggctg gcggcagacg cagcagattg ctggcaccgt tatcgctctt
     6841 cgccgcaggg gcgttaccac ctttcagcat ctggtctaat ggcagaacca tcaggttgcc
     6901 acctttatcg ttaaccagca ctttgcgggt gttgcccaac actttttcca tcgtctcgat
     6961 atacagacgc tcgcgagtaa tttccggcgc ggctttatat tccggcagaa gtttagcaaa
     7021 gcgcgccact tcaccctgag cttccaggat ggtctgggcc ttgtacgcac gcgcctcttc
     7081 gaggatacgt tgcgcctgac cgttcgcacg cggctgaact tcgttggtat acgcttctgc
     7141 ttcacgaatg tattgctgtt cgttttcacg cgcggcaatc gcatcgtcaa acgccgcttt
     7201 tacttcttcc ggcggacgag cagcctggaa gttgacgtcc agcagcgtga tacccatgtc
     7261 atacggacga atcgtctctt ccagttcgcg ctgagtatcg ctacgaatca cggtacgacc
     7321 ttccgtcaga atgcggtcca tggtgtattt accgataacc ccgcgtaggg cgctgtcggt
     7381 agcctgacgc aggctgtcat ccgggctggt cacgctatac agatattttt ccggatcggt
     7441 gacgcggtac tgcacgttca tctcaacgcg cactacgttc tcgtccgacg tcagcatcac
     7501 accagaagct gccagttcac gcacggcttc cacgttcacc ggtttgactt cgtcaataaa
     7561 cgtcggtttc cagttcagac ccggctcaac cagatggctg aatttaccaa agcgtgttac
     7621 cacgccgcgt tcggcttctt taatggtata gaaaccactg gccgcccaga taatgacaat
     7681 cgctgccgct gcgatggtaa cgacgcgacc gccaagctgc gggcgcgggc cttgcgatga
     7741 actgccaccg ccagatccgg tgcctttacc gccacccaga ccaccgagct ttttgctcag
     7801 tttgcggaag atatcatcta aatcaggtgg cccttgatcg cgaccgcctt tgtttccatt
     7861 tccctcagag ttgccgccag gtttgctgct tccccacggg tcgcggtctt gtccgttatt
     7921 accgggctga ttccacgcca tgtttgtgct ccatatttgt tatgcggtga tccctgttgt
     7981 ctttcgacgc cagaggatat gactccacgc ttcagacgct acgccgttag atcaggtaat
     8041 cgatcaacgc cggttcttgt ttacagaggc gacgccagtc aacgatcggc atacgaactt
     8101 gcagacttac gctgccgtcc tcctccatcc actctttttc tattgcctga agctgataaa
     8161 aacgacttct cagacgccct tcctgcggcg gcagacgcaa tgtatgctgc gccacctcgc
     8221 cggaaagacg ctccgtcaaa gcctgaaaaa gctgtggtat ccccgctccg gtctgcgcag
     8281 aaagccagac acggatcggt ttgttctctt catctctgtc aatacgcggt tcgaaatcgt
     8341 ccagcatatc gatcttgttc atcaccagca gggttgggat ctcgtgagca tcaatctctt
     8401 caagaaccgt attcaccgct tcgatgtttt cttgtacacg cacatccgcc gcgtcaatga
     8461 cgtgcagcag caatgtggct tgccgcgtct cttgtaacgt ggctttaaat gccgccacca
     8521 gatcgtgcgg caggtggcga ataaacccta cggtatctgc caacacggtt tcaccgacat
     8581 ccgcaacgtc aatacgccgc aacgtcgggt cgagggtggc aaacaactgg tctgccgcgt
     8641 agacccgcgc ttcggtgatg cgattgaaaa gggtagattt accggcgttg gtatatccca
     8701 ccagcgaaac agtaggaaca tcggctttga tacgcgattg ccgcccctgc tcacgctgct
     8761 tttcaactct ttccaggcgc gactgtatct gcacgatgcg attacgcaac aaacgacggt
     8821 cggtttcgag ctgggtttca cccggaccac gcaaaccgat cccgcctttc tgtctttcaa
     8881 ggtgggtcca gccacgcacc aggcgcgtag ccagatggcg caactgcgcc agctcaacct
     8941 gcaacttacc ctcatgggta cgcgcacgtt gggcgaaaat atctaaaata aggccggtgc
     9001 ggtcgatgac acgacactcg cacaaacgct ccaggttacg ctcttgcgcc gggctcaggg
     9061 catggtcaaa aagaacgacc gaagcacccg tcgcttttac ggcttccgca atttcaactg
     9121 ctttaccttc acctacaaaa tactttgggt gcggcgcttt acggctaccg gtaatcacct
     9181 gcaatgcttc gacaccggcg gaagagacca gagattcaaa ctcctggagg tcttccatat
     9241 ctttgtcttg cgtaaaatag atgtgtacca gtaccgcctg ctcaccagca tcataacggt
     9301 caaacaagcg tataaccctc taaatagatc agcggggaac gcaggatcgc tggctccccg
     9361 tgtaaaaaaa cagcccgaaa ccttattcgg tttcttcgct gtcctgttgc gcggaagtat
     9421 tctgcgcgct gctaccatga tgatagttac tgctggtacc gccaccggcg ttgttactgt
     9481 gatgagaaac cgggcgagac gggacaacag tagaaatcgc gtgcttgtaa accatctggc
     9541 tgaccgtgtt tttcaacagg atcacgaact gatcaaaaga ctcgatttgt ccttgcagct
     9601 taataccatt caccaaataa atagaaactg gaacacgttc ccgacgcagt gcgttcagga
     9661 acggatcttg taaagattgc cccttagcca ttctctcttt tccttatatg cttatttgta
     9721 ctttgaacct ttcgattctg aaaaaattgc gcacgatacg tctcaattgt acacattcag
     9781 cctgcgatag caccaacaac ctgtaatact tcgtcacgcg cctgttctgg tttttcactg
     9841 tcaagccagt gaaccccttc ccaaccacgc agccaggtta tctgccgctt cgccaactgt
     9901 ctcgtggcgc aaacacctcg ataaaccatt tcatcgtatg agatttcgcc ttcaaggtaa
     9961 gaccacatct ggcgataacc cacgcaacga atggaaggca aatccgtatg caaatctcct
    10021 cgggcaaaaa gcgcccggac ttctgcttca aaacctgaag ccaacatctg atgaaaacgc
    10081 tgctcaattc gttgatggag cagttcacgg ctcgccgggg cgatggcgaa ctgatgcacc
    10141 tgatacggta gagcgtctcc tgacgtttgc gtcagttccg ttaaagtttt acccgaaatg
    10201 aaaaaaactt ccagtgcccg ggaaagcctt tgtggatcat ttggatgaat ccttgctgcc
    10261 gcaaccggat ctacctcctg aagttgacga tgcaatgact cccaaccttg ctctgccgcc
    10321 tgttgctcaa ttctggcccg tacttccggg tccgccgacg gtagcggcga caacccttcc
    10381 agcaatgcct tgaaatacaa catcgtaccg cccactaata gtgggatccg ccccgccgcg
    10441 gtgatatcgg ccatttccgc cagcgcatcg cggcgaaaat cagcagccga gtaagcctgc
    10501 gacggatcgc gaatattcag caatcggtgc ggcgcggcga gtaactcttc agcgttcggc
    10561 ttcgccgtcc cgatatccat ccctttgtaa ataagggcag aatcaacgct tatcaactct
    10621 actggtaaaa ttttacgcag ctcaatggct aacgctgttt taccggaggc cgtcggcccc
    10681 atcaaaaaaa tcgccttagg caggctcgcc ttactgatat cactcatctt tcagggcttt
    10741 tatcgccgga tgtaaatcaa cagattgtaa cagaccaccc ggcggcgttt tcacaagttg
    10801 cggacataac cgttccacgt ccgccagcag ggttatggcc tgtgccattg accactgcgc
    10861 atgttcgctc atcagatttc gtgcaatcca ctgcgcaata ttgccaggtt cgaatacgga
    10921 ctgcttcgcc aggtagccta tcagttcagg aatcaagatt tgtaaatttt gttggcgtaa
    10981 gggtaaaggc acggccctga tggtcacatg ctgcgcatcg gactggaaat caatacccaa
    11041 ttccgccagg gcagactgcg ctttttctaa tgccgatttt tcttcgccag aaacttttag
    11101 ccgcaacgga ataagcagcg gttgggcgca aactggcact tcacccggcg tcaattgtgc
    11161 ctgacgcagc caacgttctg ccactggcaa ggataaaagt gaaatgttgc catcgcgctc
    11221 aagcaacgca cagtcggaat ggacgatagt cagtacccga ccaaaactct gactgttcgc
    11281 cgcaagtgca ggttcctgcg gttctggcgc ttttggtttt tgcatcggcg cgggcgtttg
    11341 caaaagctgg cgatacactt caccttgctg tttctggtaa cctggctgcg cattcggcca
    11401 gggggcagcc ggacgactgc ctgaggaggg cgcaggagtg taacgcggag ctactggctc
    11461 acgaactgcc ggttcagcaa aatgattgcg ccccgccgcc acgcgatttt ccggaatggc
    11521 acgcggtgca ggttgtggtt catcgtccag cggtagcggc gtttccagtt gctgttgtag
    11581 cacgctcagc acgccctgat agataaagtc atgcaccaga cgcgactgat ggaaacgcac
    11641 ttcgtgtttg gcagggtgca cgttgacgtc cacctgatgc gggtcgatct ccagatacaa
    11701 cacaaatgcc ggttgctgat cggcccccag tttgtcttcg caggcctggc ggatcgcgtg
    11761 attgatcagg cgatcgcgca tcatgcgacc gttcacgtag caatactgaa tttctgccag
    11821 tgcgggcgtg gtgtgatttg gatcggccac ccagccgcgc agcgtgagat cgccgtgttg
    11881 ccattcaatc gccagcgctt gttcgagaaa agcggtgccg caaatcgcgc ctaagcgccg
    11941 ttctttttgc ccgccttccg gcactgcgcg gtactggcgt acaattttgc cgttatgcga
    12001 caggttgata gtgacgtcga aacgcgccag cgcaatgcgg cggatgattt catcgatatg
    12061 gttaaattcg gttttctcgg tgcgcaggaa tttgcgccgc gccggggtgt tgtagaacag
    12121 gtccagcacc tccagcgtcg ttcccaccgg atgcgccgcc ggtttaaccg tcacgtccat
    12181 attgcgccct tcggcataag cctgccaggc ttcctgctgt tctgcggtgc gtgaagtgag
    12241 cgtcaggcgg gaaaccgaac tgatactcgc cagtgcctca ccgcgaaagc ccaggctgat
    12301 aatggcttcg agatcgtcca gagaggcgat tttactggtg gcatgacgcg ccagcgccag
    12361 tgccagctca tcttttttga taccgcagcc gttatcacga atgcggataa gtttcgcccc
    12421 accgcgttcg atatcaatat cgatacgcgt cgcacctgca tcgaggctgt tttccaccag
    12481 ttctttgact accgacgcag gtcgctcgac cacctcacct gcggcaatct ggttcgccag
    12541 ttgtggcggt aagacctgaa ttggcatcaa tcgtccttag tttggcagcg tgcgatctgg
    12601 cgtcgtcacc gtactggccg tttgtgccgt tgcaccctgc ggcgcagatt gcatcggatg
    12661 cgcaaggaag taattgcgca ggcctttgta aatggcttct gccagctgtt gttggtaatc
    12721 gtcgctcgcc agcaaacgtt cttcgctgtt gttactgata aaaccggttt cgaccagtac
    12781 tgacggaata tccggcgaac gcagaacgcc gagactcgcg tgttctggtc ggcgtttatg
    12841 tatttcgcca atgcgttgca actgactgat catactggtc gctacatcat accctacccg
    12901 ctgggaatga ccgaactgta aatccagcac cgcctggctt aaataggggt cggcctgact
    12961 gttcgccagc acatcacccg ccccgcccag cagctccgac tgcttctcat gctgttccag
    13021 ccagctggcc atttcgctgt tggcgcgacg gtttgagagc acccatacag aggcaccggt
    13081 ggcactacgg tttggtgcgg catcggcatg aatcgacaca aggaaattgg cgttttgctt
    13141 acgtgccaca tcactgcgcc ccatcaccga gataaagtaa tccccgtcac gggttaaaac
    13201 gcccttaaac atcggatcgt tattgagcaa ggtacgtaat ttacgcgcaa tggcgatggt
    13261 gacatttttc tcccgtgtac caccagggcc gatagcgcca gggtcctgac cgccgtgtcc
    13321 ggcatcaata gcgatgataa ttttatcgcc tgtgttagcc gtcgcgcgcg ctgccggacg
    13381 cgttaccgta ttactgctga taacacccgt agtgcggtta ctttccgttt taaacggatt
    13441 gcgcgccggt tcgctgacgc gcggtgcgac aaccgcaggc gtttcaacgc gtttcgcaac
    13501 cattggcgga ggcgatggcg gcgcatcggc gttaatcgta aagacaacag tgtaattgct
    13561 gccattctgc cgcttcaccg cttcggtttt accgttttcg gtgagatcga ccaccagccg
    13621 tagcgtttgt gcatctttag gcgttccgga gcgaatcgct ttcaccaggt tattaccgct
    13681 aaacaacaac ggcagcccct gaattacccc cgtttgtttg atatcgagcg ccacggtgcg
    13741 tttgctttga tggctaaacg cataatcagg atcgccaata aaactcaacg ttatccgcgc
    13801 ctgttgatta ccgttagaaa cctgaatatc agagagcgtc gcggcccccg cctgcgcgca
    13861 cagcagcagc aacgtcgcta ctaaccaatt tctgatgcga tacatcatcc cgccaccttt
    13921 caaaggttaa ccggctaaac gcgccagcaa caattcaccc gcagaggaaa ccgcactcac
    13981 gcgcgcctca cggccttgtg cctgataatc aatgtgtatt tcgacatccg ggtcaggaag
    14041 aacacctgta ccttgttgtg gccactccac caggcagatg gcatcgttgg caaaataatc
    14101 gcggatcccc ataaactcca gctcctcggg atcggcaagg cggtacaaat caaagtgata
    14161 aaccattaaa ttgtcgagcg tatagggttc gaccagcgta taagtggggc ttttgacatt
    14221 accctgatga cccagagcct gtaaaaagcc ccggctaaag gtggttttac ctgcgcctaa
    14281 atcgccatac agatagatta cggttgcgcc atcgcaggct ttcgctaccc gctcgcccag
    14341 gtctaatgtt gcctgctcat cagggagcgg aattactcga ttcatcatgg tttttatcag
    14401 tcacttccgg gttaacaata cgctgtagcg tggaaaagag atcggttgcc agcatcccgc
    14461 gcgttccaaa acgcgccgcc agtacgtcag ctgccgcacc gtgcgcgaca cagcccgcac
    14521 aggctgcatc ataagggctc attttttgcc caagcaatgc gccaataata ccagagagca
    14581 catcgcccat gccgccgctc gccatgcctg catttccggc atcaataatg cctaaagcgt
    14641 caggatgggc ggcgaccacg gttccggcac ctttcagcac cgctacgccg ccataacgtt
    14701 gtaccagacg ttgggcgcaa tgtaagcggt cactttcaat ttcagcgacg gaacatccta
    14761 acaaccgtgc ggcctcacca ggatgcggcg tgatcacgcg attgtgacgc ttatcgggat
    14821 tgattgccag caggttcaat gcatcggcat cccacaacat cggtttgcga aaattctcaa
    14881 ctttttgcag ggcttttttc ccccactctt gctggcccag accgggacca atcaccacca
    14941 catcggccca ttccaggctt tcggtaagag agcccatagt cagttcatgc accatcaatt
    15001 ccggtcgtgc agtcagcagc ggcgcaatgt tctcactgcg ggtcagtact cggactaaac
    15061 cagcaccagc acgcagcgcc gcttcccccg tcatacgaat agcccccgcc gtgccgtgat
    15121 cgccaccaat aatcaccagc cgcccgtgat cgcctttatg cgaagtcggg cgacgcggta
    15181 tcagccactg agaaagttgt tctgccgaaa accgctgaat tttcgtctcc tgacctgcca
    15241 gccatctatc cagccgcagt gagtcaaaat gcagttgtcc ggtaacatcc cgcgcttttc
    15301 cagtgagcaa acctggtttc agcgcaataa aagtgatggt gtgatcggcg ttgattaccg
    15361 cgcctggcgt agcgccagtt tcagccagaa ggccggaagg gatatcaacc gccacaatcg
    15421 gcgcaggatg ggaattagcg tggtcgatta actggctaat ggattcgcgg ggcgcttgct
    15481 gcaaaccggt gccgagcagc gcatcgacaa tcagatctac cgattcgggc cagacaatat
    15541 tcgaagcatg gatctcgcct cccgcgttta accatgcttc gcgtgccagc gcggcctctt
    15601 ccggcaacgg tttgtcactt tcctgggcca gcagcgtgac atcaataccg atcgctttgg
    15661 ccagccgcgc gaccacgtag ccatcgccgc cgttattacc atgaccgcac agcaccagcc
    15721 agtggcgggc gtcaggataa gccgaacgac acacctggaa tgcggcttcg ccagcgcgaa
    15781 gcatcagctc atagagtgtg agccccagcg catctgccgc ctcgcgttct ccgcggcgga
    15841 tatcgtcggc gtaccagacg gtgtgtggta tacttacggg gtttttcttc attgtatggt
    15901 ccgtcatgtc agagcccctc gatctcaatc agttagcgca aaaaattaaa cagtgggggc
    15961 tggaactggg ctttcagcag gtaggtatta ccgataccga tctcagcgag tccgagccca
    16021 aactgcaagc atggctggac aaacaatacc acggcgaaat ggactggatg gcgcgtcacg
    16081 gtatgctgcg tgctcgtcct cacgaattat tgcccggtac gctgcgcgtg atcagcgtgc
    16141 ggatgaatta ccttcctgct aacgccgcat ttgccagcac gctgaaaaat cccaaactcg
    16201 gctatgttag ccgttatgcg ctgggccgtg actatcacaa gcttctgcgc aaccgactca
    16261 aaaagctggg cgagatgatt cagcaacatt gtgtttcgct gaattttaga ccgtttgtcg
    16321 attctgcgcc tattctcgag cgcccgttag ctgaaaaagc aggtcttggc tggacaggta
    16381 agcactcact tatcctcaat cgcgaggccg gttcgttctt ctttttaggc gaattgctgg
    16441 tcgatattcc gctgcccgtg gatcaaccag tcgaggaagg atgcggtaaa tgcgtggcct
    16501 gtatgacgat ttgcccgacc ggtgccatcg tcgagccata taccgtcgat gctcgccgct
    16561 gtatctctta tctcaccatc gaactggaag gggagatccc ggaagagttg cgaccgttaa
    16621 tgggaaaccg tatttacggt tgcgatgact gccagcttat ctgcccgtgg aatcgctatt
    16681 cgcaactcac tacagaagac gatttcagcc cgcgtaagcc gctacacgca ccggaactca
    16741 ttgagttatt cgcctggagt gaagagaagt ttttaaaagt cacggaaggt tcggcgattc
    16801 gccgtatagg tcacctgcgt tggctgcgta atatcgccgt agcattaggc aatgccccct
    16861 gggatgaaac gattttgaca gcgctggaaa gtcgtaaagg tgagcaccca cttcttgatg
    16921 agcacatagc gtgggcgatt gcgcagcaaa tcgagagacg aaatgcgtgc gtggtcgaag
    16981 tgcaattacc gaaaaaacag cgtctggttc gggtgattga aaaagggtta ccgcgtgacg
    17041 cctgattcat ccacagcttg tgaataaaaa taaaaacgca ttgcaattca agccggaaaa
    17101 aatcgtcaag tgatcgatac gacaaaacga aatcttaatt tttaacttat ctatcaatag
    17161 gttaaaaaaa tactattcac cgtgcgaagt ttctttgaaa ttaagattac ggcctgaggc
    17221 tgtggataac tctgtttaca agagtttgtc agagacaaca aaaaccagca ataacgcatc
    17281 gcgtcgctgt ggatatttta ttgagagaag aatttggagc gggaaacgag actcgaactc
    17341 gcgaccccga ccttggcaag gtcgtgctct accaactgag ctattcccgc ttgggtggtc
    17401 tgtgctttac agcactttca aattttggag cgggaaacga gactcgaact cgcgaccccg
    17461 accttggcaa ggtcgtgctc taccaactga gctattcccg cttgggtggt ctgtgctttg
    17521 cagcactttt caaattttgg agcgggaaac gagactcgaa ctcgcgaccc cgaccttggc
    17581 aaggtcgtgc tctaccaact gagctattcc cgcgtactac ttaattttgc tttcgtaatt
    17641 ttcgcatttc tgcgtcgtta cgggaggcgc attatacgag aaatctttcc ctctgcaacc
    17701 cccctaaaaa attttttttg agtttttatc tcaagtgctg aaattatcag caaggcgttc
    17761 aatttagcgt caaaatcacg ccgccgcaag tggattccgg cgcttacgtg accaggaaaa
    17821 attttacagc ttgataaaat gctcgcggta gtaagccaac tccgccaccg attcacggat
    17881 atcatccatc gcctgatgcg tcccctgctt ggtaaagccg tcaagaattt ccggcttcca
    17941 gcggcgcgcc agctctttca gggtgctaac atcgagatag cggtagtgga agtaggcttc
    18001 cagctccggc atgtatttaa acaggaagcg acggtcctga ccgatgctgt taccacaaat
    18061 cggcgatttt cccgcaggca cccactgttt taagaattcg agcgttgcca gttcagcttc
    18121 acgatcgccc atcgtgctcg ctttcacacg ctctaccagc ccgctggcgg tatgggtgcg
    18181 cacgttccag tcatccatca gcgccagctg ttcatcggac tgatgtactg caatggtcgg
    18241 cccttctgcc agaatattca ggttggcatc ggtcaccagc gtggcaatct caataatgcg
    18301 atcgcgctcg ggatccagac cggtcatctc aagatcgatc caaatcaggt tgttttcatt
    18361 ggcactcatg ctattttcca cccattcaca gtgactatat tgatccacaa ttgcgtgtat
    18421 catagatgtt ttgcccatca ggggcgacca ggagtctgta cgattgagta aaaataaact
    18481 ctccaaaggc cagcagcgcc gcgtgaacgc caatcaccag cgtcgtctta aaacgtctaa
    18541 ggagaagccc gactacgacg acaatctgtt tggcgagcct gatgaaggta tcgtcatcag
    18601 ccgctttggt atgcacgctg atgtggaatc cgccgatggc gacgttcacc gctgcaatat
    18661 tcgccgtacc atccgttcgt tggtgaccgg cgaccgcgta gtctggcgtc cgggtaaacc
    18721 ggcggcggaa ggcgtaaatg tcaaagggat cgtggaagcg gtgcatgagc gcacctcagt
    18781 gttgacgcgc ccggatttct acgacggtgt gaaacctatt gctgccaaca tcgaccagat
    18841 tgtcattgtc tccgccattt tgccggagct gtcgctcaat attatcgacc gttacctggt
    18901 ggcctgcgaa accttgcaga ttgagccgat tattgtgctc aacaagatag acctgctgga
    18961 cgacgaaggc atggagttcg tcaacgagca gatggatatc taccgcaata tcggttatcg
    19021 cgtattgatg gtttccagtc atactcagga tgggctgaaa ccgctggaag aggcgttgac
    19081 cgggcgcatc agcatttttg ccgggcagtc tggcgttggc aaatccagcc tgctgaacgc
    19141 gttgctgggt ctgcaaaaag agatcctgac caacgatgtc tctgacaact cggggcttgg
    19201 tcagcacacc acgaccgccg cacgactgta tcacttcccg cacggcggtg atgtgattga
    19261 ttccccaggt gtgcgtgagt ttggtctctg gcacctggag ccggaacaaa tcactcaggg
    19321 ctttgtcgaa ttccatgact atttaggtct gtgtaaatat cgcgattgca aacacgatac
    19381 cgatccgggc tgcgctatcc gggaagcggt tgaggaaggg aaaatcgcgg aaacccgttt
    19441 cgaaaactat caccgtattc tggaaagcat ggcgcaggta aaaacgcgta aaaacttttc
    19501 tgatacggat gactgacaat taagctaacc gtcgttagaa tcgtcccctt tttttcagga
    19561 tccggcatgt aggccggatc aggaacgaca aaacaatggc ctggaggcta ccttgttaaa
    19621 ttcatttaaa ctttcgctac agtacattct gccgaaacta tggcttactc gcctggcggg
    19681 ttggggcgca agcaagcggg caggatggct gacaaaactg gttatcgatc tgttcgttaa
    19741 atactacaag gtcgacatga aagaggcgca aaagccggac accgccagct accgcacctt
    19801 taacgaattc tttgtccgtc cgctgcgtga tgaagtacgc ccaatcgata ccgatccgaa
    19861 tgtcctggtt atgcctgccg atggcgttat cagccagttg ggtaaaatcg aagaagataa
    19921 aatcctgcaa gccaaaggcc acaactacag cctcgaagcc ctgctggcag gcaactatct
    19981 gatggcggat ctcttccgta acggtacgtt tgtgaccact tacctctccc cacgtgacta
    20041 ccaccgcgta cacatgccgt gcaacggtat tctgcgtgaa atgatctacg tgccgggcga
    20101 tctcttctcc gttaaccatc tcacggctca gaacgtgccg aatctgtttg cccgtaacga
    20161 acgcgtgatc tgcctgttcg ataccgaatt tggcccgatg gcgcagattc tggtcggtgc
    20221 gacgattgta ggcagcattg agacggtatg ggcgggcacc attacgccgc cgcgcgaagg
    20281 catcatcaag cgctggacct ggcctgccgg ggaaaacgac ggttctgtgg cactgctgaa
    20341 aggccaggaa atgggtcgct ttaaactagg ctctaccgtt atcaacctgt ttgcaccagg
    20401 taaagtggac ctggttgagc aactggagag cctgtctgtc acgaaaattg gccagccgct
    20461 ggcagtatct accgaaacct ttgttacgcc agatgctgaa ccagccccgc ttcctgctga
    20521 agagatcgaa gcagaacacg acgccagccc attggttgac gataaaaaag accaggtcta
    20581 atccatcaaa ggaaacgctg acgtgcgcct gattatcact tttctgatgg cctggtgcct
    20641 cagttggggg gcgtacgccg cgacggcccc cgatagcaaa caaatcaccc aggaactgga
    20701 gcaggcaaaa gcggcgaaac ctgcgcagcc ggaagtcgta gaggcgctcc agtctgcctt
    20761 aaatgcgctt gaggaacgaa aaggttccct tgagcgcatc aagcaatatc aagaagtcat
    20821 tgataattac ccgaaacttt ccgccactct gcgcgcgcag ttaaacaaca tgcgtgacga
    20881 gccacgcagc gtgtcgccgg gaatgtctaa cgatgcgctg aatcaggaaa ttctccagat
    20941 cagcagtcag ttgctggata aaagccgtca ggcccagcaa gagcaggagc gcgcccgcga
    21001 gattgccgat tccctgaatc aactgccgca acagcaaacc gatgcccgcc gtcagttgaa
    21061 tgaaatcgag cgccgcctgg ggacgcttac cggcaatacc ccgctcaatc aggcacaaaa
    21121 tttcgcgttg cagtctgact ccgcacgtct gaaagcgctc gttgatgaac tggagctggc
    21181 gcagctatct gccaataacc gccaggaatt agcgcgcttg cgctcagagc tggccgaaaa
    21241 agagagccag cagctggatg catatttgca agcactacgc aaccagttga atagtcaacg
    21301 ccaactggaa gcggaacggg cgctggaaag taccgagcaa ctggcggaaa gtagcgccga
    21361 tttgccaaaa gatatcgtcg cccagtttaa aattaaccgc gaactatcgg cggcactgaa
    21421 tcaacaggcg cagcggatgg atctcgttgc ctcgcaacag cgtcaggctg ccagccagac
    21481 gttacaggtc cggcaggcgt tgaatacgct gcgtgaacag tcgcaatggc tgggatcatc
    21541 taacctgctc ggcgaagcgt tgcgggcgca ggtggcacgg ctaccggaaa tgccaaaacc
    21601 acaacagctt gataccgaaa tggcgcagtt acgtgtgcaa cggttacgtt atgaggatct
    21661 gcttaataaa cagccgctgc tacggcaaat ccatcaggcc gacggtcagc cgctgaccgc
    21721 tgagcaaaac cgtattctgg aagcacagct acgcactcag cgtgagttgc tgaactcatt
    21781 gttgcagggt ggcgacacgc tactgctgga gctgaccaag ctgaaagtct ccaacgggca
    21841 actggaggat gcactgaaag aggtgaacga agcgacgcac cgctatctgt tctggacctc
    21901 tgacgtgcgc ccgatgacca tcgcctggcc gctggaaatc gcccaggatc tgcgtcgtct
    21961 catttcgctg gacaccttca gtcagttggg caaagccagt gtgatgatgc tgaccagtaa
    22021 agagacgatt ttgccgctgt ttggcgcgtt gattctggtc ggttgcagta tttactcgcg
    22081 ccgctatttc acccgttttc ttgaacgttc ggcggcgaaa gtcggcaaag tgactcagga
    22141 tcacttctgg ctgacgttac gcactctttt ctggtcgatt ctcgtcgcgt caccgctgcc
    22201 tgtgctgtgg atgacgctgg gttacggctt gcgcgaggcg tggccttatc cgctggcggt
    22261 cgcgattggt gatggcgtaa cagccaccgt gccgctgctg tgggtagtga tgatttgcgc
    22321 cacctttgct cgcccgaacg gcttgtttat cgctcatttt ggctggccgc gcgaacgtgt
    22381 ttcccgtggg atgcgttact acctgatgag catcgggctt attgtgccgc tgattatggc
    22441 gctgatgatg ttcgataacc tcgacgaccg cgaattttcc ggttcgctgg gacggctttg
    22501 ctttatcctc atttgcggtg cgctggcggt ggtcaccctc agcctgaaaa aggccgggat
    22561 cccgctgtat ctcaacaaag aaggcagcgg cgacaacatt accaaccata tgctgtggaa
    22621 catgatgatt ggcgcacctc tggttgccat tctggcgtcg gcggtgggtt atctggcaac
    22681 ggcgcaggcg ctgttagcga ggcttgaaac ctcggttgcc atctggttcc tgctactggt
    22741 ggtttatcac gttatccgcc gctggatgct gatccagcgc cgcaggctgg cgtttgaccg
    22801 ggcaaagcat cgccgggcag agatgttagc gcagcgtgcg cgtggcgaag aggaagcgca
    22861 tcatcacagt agcccggaag gagcaattga agtcgatgaa agcgaagtcg atctcgatgc
    22921 catcagtgcg caatccttgc ggctggtgcg ctcaattttg atgttgatcg ccctgctttc
    22981 ggtcattgtg ctgtggtcag aaatccattc cgctttcggc ttcctcgaaa atatttcgct
    23041 gtgggatgtc acttccacgg tacagggcgt agaaagcctg gagccgatta ccctcggtgc
    23101 ggtgctgatt gccattctgg tgtttatcat caccacgcag ctggtgcgca acctgcccgc
    23161 gctgctggaa ctggcgattc tgcagcacct ggatttaacg ccgggtacgg gctacgccat
    23221 caccaccatc accaaatatc tgctgatgct aattggcggg ctggtcggct tctcgatgat
    23281 tggtattgag tggtcgaaat tgcagtggct ggtcgccgcg ctcggtgttg gtctcggttt
    23341 tggtttgcag gaaattttcg ccaactttat ctctggcctg attatcctgt tcgaaaaacc
    23401 gattcgcatt ggcgatacgg taacgatccg cgatcttacc ggtagtgtga cgaaaattaa
    23461 cacccgcgcc accaccatca gcgactggga ccgcaaagaa attatcgtgc cgaacaaggc
    23521 gtttattacc gagcagttta tcaactggtc gctctctgac tcggtcacgc gcgtggtgtt
    23581 gacgattccg gcccctgcca atgccaacag tgaagaagtg acggaaatcc tgctcaccgc
    23641 agcgcgtcgc tgctcgctgg tgatcgacaa cccggcgccg gaagtcttcc tggtggatct
    23701 gcagcagggg attcagattt tcgagctgcg tatttacgcc gctgagatgg gtcaccgtat
    23761 gccgctacgc catgagattc accagctgat tctggctggc ttccacgccc acggtatcga
    23821 tatgccattc ccgcccttcc agatgcgtct ggaaagcctg aatggtaaac aaacggggag
    23881 aacattaact tctgcgggca aaggtcgtca ggcgggaagt ttgtaaggtc tttgtggccg
    23941 gatgaaccca tccggctcac aagcaaaact gatagcgcag aaaaaactgc cacaaccagt
    24001 agccgaaaat agccagcgcg gtggcgtaca taaaccagcc gcgttttttt cgccacaaag
    24061 cgaaccagag aaaagggaaa aagagcggta acgtcattat cacaccgaca tcgcgagtat
    24121 cgtcatcttc gagatacatc aaaacatcac agacgttgtt aatcccgcgt ccatcaatca
    24181 tccactcttt gtccagcgcg gaggtaatgg ttatccagag gaacgccaca atgaaccaga
    24241 tgcagcacaa gacagacatg cgcgcactca tgaggtaacc catagtttaa tttcgctata
    24301 gcgcatacga aatctcacta attccctgtc attacgccct atcgtcaggc cgacgatagg
    24361 gcatttgatt actcagccac ccactgcgga ttttttacca ctctgctgca atagttctgg
    24421 taaatcgcca tcgctaacag cgagaagaag acagggccgc caatcatcca caatgtgctg
    24481 tcccagtctc cggcttcaac cacaggttga atgatggtga agacgttcgc aaatgtcacc
    24541 accagtacga caaccactgt cgcaatcatt gccgacatac gcgttttgaa aatcacaaac
    24601 ggtctgtcga gatcctgacg tgctttaaag aacgggaaag ccagcgcgag gaacaggtaa
    24661 ggaagcgtca tagacacgtt cgccatcagc gtcagcttgt taaagaacgc cgatgcggta
    24721 ccgccaccaa acgaaaccag caggatgaag acagtaacca acccacactg catccacatg
    24781 gcgatagacg gcatccccat cgcattcagg cgcgtcatcg gttccggcca caatgctttc
    24841 ggcgtcccct ggatgatggc tttcagcggt gaatagcaaa gcgtaaagaa cgcaccggta
    24901 taagcgagga acatcgacag tccggtaata cgcgcaaacc atacgcccag cgacaatgac
    24961 gcttcaggtg acaaatgcag tgcgttaccc agcgtcaccc cgaggctctt catcagcaca
    25021 taggtaatat tgccgaggtt aacggaacca ttacttaata cctgctgcca gtttgtgctg
    25081 acgccccata aaaatattgc cagcgaataa ccgattgaaa taacaatagc ggcaaaaaca
    25141 atacctttgg caaagttctt ttctggattt tccgttttat caaccagacc accaaccgct
    25201 tcaattccgc cataggcaaa aatggcaaat accacaaacg atagcattgc cagaccggac
    25261 tgataacctg ggttcggtga tgcaaggaaa ttaatatcct gcgcgaaatg cccaccattt
    25321 aataacaaaa tagtaatgct tactaacagc aatactaaat tcagacacat tactgcaata
    25381 ccgcccaccg cagtaatgcg ggcaatttta ttaatccctt ttgaagcaac gacggtgacc
    25441 agaatcatcc atgccactgc cagcagacca accacctgcg taggctccag tccggcaata
    25501 tgccagtgct gggtcatgtc gctaccgtag aggaatgttg agaacggtac ccaaactttc
    25561 gcggaggtac tcaccatcca gatgatataa gaggaaaacc acataaacgt accaatgaag
    25621 gcaaaacgtg gtccgacact attattcatc caggaataga taccgccttc ttctttgcga
    25681 taagcagctc ccatttcagc catcattaag gcgaatggaa taaagaataa caatgcagaa
    25741 aatatataaa aggaaatcgc actataaccc attaagtaat aagccgatgg gctattggca
    25801 aatccaaata cagaagtaaa gatcatcaat atgagtccta tcagactcat cttttttatc
    25861 gagtgaggca tgaaaccatc cttcatttca gcgcgtgtat aaaacgcgcc cactgaactg
    25921 cactgcccaa cattgagcgg gcagcgtaaa aaccaataca gaattgatac ctggatgata
    25981 tcaaatatcc agtcttaaat tgtggccttt agcggaaaaa tgaaaacata tgctacagaa
    26041 tggcgcttat cacgccattc ttcgctgtta attcagtaat ttttcagaat tatgcccggt
    26101 caacgctaaa ggcgatgact tcagccagtg tctccgcgcc cagcgccaac atcaccagac
    26161 gatcaacacc taatgccacg ccggaacagt caggcatacc gactttcaag gcttcaatca
    26221 gattctggtc aatggggtgc tgcggcaaac cgcgcgccgc gcgcttacgg ttatcttgtt
    26281 caaagcgttg ttgctgctca cgggcatccg tcaattcatg gaaaccattc gccagctcaa
    26341 tacctttata ataaacctca aagcgttcag cgacccgatg atcttcggta ctgatttgcg
    26401 ccagtgatgc ctggctggct ggaaagtggt acacaaaggt cggtttttct ttgccaatat
    26461 ttggctctac gccaaaggta aacagcaatt gtagaagcgt gtcgcggtct tcttcagtat
    26521 cggcaacgtt gctcaaatcc agtttcgctg ccacttcccg cagttgcgtt ttgtcggcag
    26581 agagtgggtc aatttccaga taacgcaaga aagcttgttg ataagaaagg ctttctgctg
    26641 ccggacagtc cagcacctgt tgcaagagat cgtccacctc gttcatcaac cggtacatat
    26701 catagtgcgg tcgataccac tcaagcatag tgaactcagg gttgtgataa cgccccatct
    26761 cttcattacg gaagctgcgg cacagctgga ataccggccc acaaccggca accagcaggc
    26821 gcttcatatg gtattccggg ctggtcatta accagagatt catcccctgc gaatgcccgg
    26881 ggccaacgaa acgtgtctca aacgggacca aatgaatatc ggttaccgtc gcctggctca
    26941 tacaaggcgt ctccacctcc agcactccac gatcggcaaa gaaacgacgg atctccgcca
    27001 taatcgccgc gcgttttaat aagttaggaa tggatgcgct cggctgccag gatgccgttt
    27061 cgctcatagt taaatctcca gtttttgaca agggcacgaa gtctactcgc aacgcgacgg
    27121 cgagacaaat tttacgcagg aataacacaa cggtgggcag tgactaaaaa aagcacgatc
    27181 tgatggttta gtaattaaat taatcatctt cagtgataat ttagccctct tgcgcactaa
    27241 aaaaatcgat ctcgtcaaat ttcagactta tccatcagac tatactgttg tacctataaa
    27301 ggagcagtgg aatagcgttc gcagaccgta actttcaggc acttaccctg aagtacgggg
    27361 ctgtgggata aaaacaatct ggaggaatgt cgtgcaaacc tttcaagccg atcttgccat
    27421 tgtaggcgcc ggtggcgcgg gattacgtgc tgcaattgct gccgcgcagg caaatccaaa
    27481 tgcaaaaatc gcactaatct caaaagtata cccgatgcgt agccataccg ttgctgcaga
    27541 agggggctcc gccgctgtcg cgcaggatca tgacagcttc gaatatcact ttcacgatac
    27601 agtagcgggt ggcgactggt tgtgtgagca ggatgtcgtg gattatttcg tccaccactg
    27661 cccaaccgaa atgacccaac tggaactgtg ggggtgccca tggagccgtc gcccggatgg
    27721 tagcgtcaac gtacgtcgct tcggcggcat gaaaatcgag cgcacctggt tcgccgccga
    27781 taagaccggc ttccatatgc tgcacacgct gttccagacc tctctgcaat tcccgcagat
    27841 ccagcgtttc gacgaacatt tcgtgctgga tattctagtt gatgatggtc atgttcgcgg
    27901 cctggtagca atgaacatga tggaaggcac gctggtgcag atccgtgcta acgcggtcgt
    27961 tatggctacc ggcggtgcag gtcgcgttta tcgttacaac accaacggcg gcatcgttac
    28021 cggtgacggt atgggtatgg cgctaagcca cggcgttccg ctgcgtgaca tggaattcgt
    28081 tcagtatcac ccaaccggtc taccaggttc cggtatcctg atgaccgaag gctgccgcgg
    28141 tgaaggcggt attctggtca acaaaaatgg ctaccgttat ctgcaagatt acggcatggg
    28201 cccggaaact ccgctgggcg agccgaaaaa caaatatatg gaactgggtc cacgcgacaa
    28261 agtttctcag gccttctggc acgaatggcg taaaggcaac accatctcca cgccgcgtgg
    28321 cgatgtggtt tatctcgacc tgcgtcacct tggcgagaaa aaactgcatg aacgtttgcc
    28381 gttcatctgc gaactggcga aagcgtacgt tggcgtcgat ccggttaaag aaccgattcc
    28441 ggtacgtccg accgcacact acaccatggg cggtatcgaa accgatcaga actgtgaaac
    28501 ccgcattaaa ggtctgttcg ccgtgggtga atgttcctct gttggtctgc acggtgcgaa
    28561 ccgtctgggc tccaactcgc tggcggaact ggtggtcttc ggtcgtctgg ccggtgaaca
    28621 agcgacagag cgtgcagcaa ctgccggtaa tggcaacgaa gcggcaattg aagcgcaggc
    28681 agctggcgtt gaacaacgtc tgaaagatct ggttaaccag gatggcggcg aaaactgggc
    28741 taagatccgc gacgaaatgg gcatggccat ggaagaaggc tgcggtatct accgtacgcc
    28801 ggaactgatg cagaaaacca tcgacaagct ggcagagctg caggaacgct tcaagcgcgt
    28861 gcgcatcacc gacacttcca gcgtgttcaa caccgacctg ctctacacca ttgagctggg
    28921 ccacggtctg aacgttgctg aatgtatggc gcactccgca atggcacgta aagagtcccg
    28981 cggcgcgcac cagcgtctgg acgaaggttg caccgagcgt gacgacgtca acttcctcaa
    29041 acacaccctc gccttccgcg atgctgatgg cacgactcgc ctggagtaca gcgacgtgaa
    29101 gattactacg ctgccgccag ctaaacgtgt ttacggtggc gaagcggatg cagccgataa
    29161 ggcggaagca gccaataaga aggagaaggc gaatggctga gatgaaaaac ctgaaaattg
    29221 aggtggtgcg ctataacccg gaagtcgata ccgcaccgca tagcgcattc tatgaagtgc
    29281 cttatgacgc aactacctca ttactggatg cgctgggcta catcaaagac aacctggcac
    29341 cggacctgag ctaccgctgg tcctgccgta tggcgatttg tggctcctgc ggcatgatgg
    29401 ttaacaacgt gccaaaactg gcatgtaaaa ccttcctgcg tgattacacc gacggtatga
    29461 aggttgaagc gttagctaac ttcccgattg aacgcgatct ggtggtcgat atgacccact
    29521 tcatcgaaag tctggaagcg atcaaaccgt acatcatcgg caactcccgc accgcggatc
    29581 agggtactaa catccagacc ccggcgcaga tggcgaagta tcaccagttc tccggttgca
    29641 tcaactgtgg tctgtgctac gccgcgtgcc cgcagtttgg cctgaaccca gagttcatcg
    29701 gtccggctgc cattacgctg gcgcatcgtt ataacgaaga tagccgcgac cacggtaaga
    29761 aggagcgtat ggcgcagttg aacagccaga acggcgtatg gagctgtact ttcgtgggct
    29821 actgctccga agtctgcccg aaacacgtcg atccggctgc ggccattcag cagggcaaag
    29881 tagaaagttc gaaagacttt cttatcgcga ccctgaaacc acgctaagga gtgcaacatg
    29941 acgactaaac gtaaaccgta tgtacggcca atgacgtcca cctggtggaa aaaattgccg
    30001 ttttatcgct tttacatgct gcgcgaaggc acggcagttc cggctgtgtg gttcagcatt
    30061 gaactgattt tcgggctgtt tgccctgaaa aatggcccgg aagcctgggc gggattcgtc
    30121 gactttttac aaaacccggt tatcgtgatc attaacctga tcactctggc ggcagcccta
    30181 ctgcacacca aaacctggtt tgagctggca ccaaaagcgg ccaatatcat tgtaaaagac
    30241 gaaaaaatgg gaccagagcc aattatcaaa agtctctggg cggtaactgt ggttgccacc
    30301 atcgtaatcc tgtttgttgc cctgtactgg taaggagcct gagatgatta atccaaatcc
    30361 aaagcgttct gatgaaccgg tattctgggg cctcttcggg gccggtggta tgtggagcgc
    30421 catcattgcg ccggtgatga tcctactggt tggtattctg ctaccgctag ggctgtttcc
    30481 aggtgatgcg ctgagctacg agcgcgttct ggcgttcgcg cagagcttca ttggtcgcgt
    30541 attcctgttc ctgatgatcg ttctgccgct gtggtgtggt ttacaccgaa tgcaccacgc
    30601 gatgcacgat ctgaaaatcc acgtacctgc gggtaaatgg gttttctacg gtctggctgc
    30661 tatcctgaca gttgtcacac tgattggtgt cgttacaatc taacgcatcg ccaatgtaaa
    30721 tccggcccgc ctatggcggg ccgttttgta tggaaaccag accctatgtt caaaacgacg
    30781 ctctgcacct tattaattac cgcctcttgc tccacatttg ccgcccctca acaaatcaac
    30841 gatattgtgc atcgcacaat taccccgctt atagagcaac aaaggatccc cggtatggcg
    30901 gtagcggtaa tttatcaggg taaaccttat tactttacct ggggctatgc ggacatcgcc
    30961 aaaaagcagc ccgtcacaca gcaaacgttg tttgagttag gttcggtcag caaaacattt
    31021 acgggcgtgc ttggtggcga cgctattgct cgaggggaaa tcaagttaag cgatcccaca
    31081 acaaaatact ggcctgaact taccgctaaa cagtggaatg ggatcacact attacatctc
    31141 gcaacctaca ccgctggcgg cctgccattg caggtgccgg atgaggtgaa atcctcaagc
    31201 gacttgctgc gcttctatca aaactggcag cctgcatggg ctccaggaac acaacgtctg
    31261 tatgccaact ccagtatcgg tttgttcggc gcactggctg tgaagccgtc tggtttgagt
    31321 tttgagcagg cgatgaaaac tcgtgtcttc cagccactca aactcaatca tacgtggatt
    31381 aatgtaccgt ccgcagaaga aaagaactac gcctggggat atcgcgaagg taaggcagta
    31441 catgtttcgc caggggcgtt agatgctgaa gcttatggtg tgaagtcgac cattgaagat
    31501 atggcccgct gggtgcaaag caatttaaaa ccccttgata tcaatgagaa aatacttcaa
    31561 caagggatac aactggcaca atctcgctac tggcaaaccg gcgatatgta tcagggtctg
    31621 ggctgggaaa tgctggactg gccggtaaat cctgacatca tcattaacgg cagtgacaat
    31681 aaaattgcac tggcagcacg ccccgtaaaa ccgattacgc ccccaactcc tgcagtacgc
    31741 gcatcatggg tacataaaac gggggcgacc ggcggatttg gtagctatgt cgcgtttatt
    31801 ccagaaaaag agctgggtat cgtgatgctg gctaacaaaa actatcccaa tccagcgaga
    31861 gttaccgccg cctggcagat tctcaacact ctacagtaaa atttcatcgg gtccgaattt
    31921 tcggaccttt tctccgcttt tccttgctgt catctacact tagaaaaaaa ccagtaagga
    31981 aacatttatg cgcctgctcc ctctcgttgc cgcagcgaca gctgcatttc tggtcgttgc
    32041 ctgcagttct cctacaccgc cgcgtggcgt gaccgtagtg aataatttcg acgctaaacg
    32101 ctatctcggc acctggtatg agattgcccg ttttgatcac cgctttgaac gtggactgga
    32161 aaaagtcacc gcaacataca gcctgcgtga tgacggcggc ctgaatgtca ttaataaagg
    32221 ctataaccct gacagagaaa tgtggcagca gagtgaaggg aaagcgtact ttaccggcgc
    32281 accaactcgc gctgcgctga aagtgtcatt ctttggtcct ttctatggcg gttataacgt
    32341 tattgcactc gatcgggaat accgccatgc gctggtttgc ggcccggacc gcgactacct
    32401 gtggatactc tcccgcacgc caaccatttc tgacgaagtg aaacaggaga tgctggcagt
    32461 cgcgacccgg gaagggtttg atgtcagtaa gtttatttgg gtacagcagc ccggtagtta
    32521 atgagtgctg agtttcagac caataatccc caatacgatt aacgccagac tcgccagacg
    32581 catcgggtta gcggactcac cgagcaacac aatgccggtg atggccgcgc cgactgcgcc
    32641 aatacccgtc cacacggcat aagccgtccc tactggtaac gatttcatcg cccaggcaag
    32701 tagcgccata ctgacaatca tcgccgtcac ggtaataaca cttggcgtca aacgactaaa
    32761 gccgtgggta tatttcaggc caacggccca taccacttcc agcagaccag caataactaa
    32821 gataatccag gacatatcag gctccggaaa attggggccg tccccggtga aaagacgcgt
    32881 ttgcaggtcg tcctgcaaag ctgatggttg aacggatatt ttgccaggtg gcaggggaat
    32941 ttcaactatt acctatgtaa ctatcgcaga aaaaacaaac ggcacgacac agctgtcgta
    33001 ccgttattgc ttattgctgc gctttcgttg ctgcgccaga aatcgcgtta ccgccatcag
    33061 aaatgtcttc accaacgcca cgcgtggtgt tgcaggcagt taatactgtt gaaagcacca
    33121 gaacagaaaa gatcgctgca attgtcttct tcaccataac gtcttccttt taggtaatgt
    33181 tatttatgtt tgcctatagc aaattaataa tagacaacat cgccaaaaat gacggaaatc
    33241 tgatgatttt tcggaagaga agaaaaatta gctggcagcg cgggagatgg agttgccgag
    33301 gtgtttgata tcttcgccga aacctcgagc ggtgttacag cccgtaagca gcgtgctggc
    33361 aagcaaaaca agaacgataa ggcgtttcat catctctaac ctgaataagt gatggtgcag
    33421 cctgcaggcc gcaccacaac cgcattactt cacgcgagag acgtattcac cagagcgggt
    33481 atccactttg atgacttcgc cgatttgtac aaacagcgga actttaacca cagcgccagt
    33541 agacagggta gccggtttgc cgccagtacc tgcggtatca cctttcaggc ccggatcggt
    33601 atccacgatt tccagttcaa cgaagttcgg cggagtaacg gagatcggct gaccattcca
    33661 cagagttacg atacactctg cctgatccag caaccatttg gcgttgtcgc caattgcttt
    33721 tgcatcagca gacagctgct cgaaagtttc gttgttcatg aagtgccaga actcaccgtc
    33781 gttgtacagg taagtcaggt tcatatcgac aacatcagca ccttcagcgg aatcagtaga
    33841 tttgaaggtt ttttctacgc gagtaccggt cagcagacga cgcagtttaa cgcgagcaaa
    33901 tgcctggcct ttacccggtt ttacgaattc actcgcttca accgcgtaag gttcgccgtc
    33961 taacatgatt ttaagaccag cacgaaaatc gttgctatag tacgttgcca taaggccctc
    34021 tgaaatttgt taattggtag ctaagccaca aaatggcgca tattgtaacc ctaaataccc
    34081 catccagaga agattggtta acgcaacttg ccgatgttgt gaccgatcct gatgaacttc
    34141 tgcgtctttt gaatatagac gcggacgaaa aactgttggc cggacgcagc gccaaaaagc
    34201 tgtttgccct gcgtgtgccc cgctcattta tcgatcgcat ggagaaaggc aatccgaacg
    34261 atccgctttt gcgtcaggta attacctcgc aagatgagtt tgttgtcgcg cccggattct
    34321 ccaccgaccc gctggaagaa cagcacagcg tagtgccagg tttgttgcat aaataccaca
    34381 accgggcgct tttgctggtc aaaggcggct gcgcggtaaa ttgccgctat tgcttccgtc
    34441 gccacttccc ctatgccgaa aatcagggca acaagcgtaa ctggcagact gcacttgagt
    34501 atgttgctgc gcatccggaa ctggacgaga tgattttctc cggcggcgat ccgctgatgg
    34561 cgaaagatca cgagctggac tggttgctca cacaactgga agccatcccg catatcaaac
    34621 gtctgcggat tcacagccgt ctgccgattg tgatcccggc acgcatcaca gatgcgctgg
    34681 ttgaacgctt ttctcattct acgctgcaaa tcttgctggt gaatcacatt aaccatgcca
    34741 atgaggtaga tgaaacattc cgtcaggcga tggctaagtt gcgccgggta ggcgttactt
    34801 tgctgaacca gagcgttctg ttacgtggtg tgaacgataa cgcgcaaacg ctggcaaacc
    34861 tgagtaatgc gttgttcgat gcgggtgtga tgccgtatta cctgcatgtg ctcgataaag
    34921 tacagggcgc ggcgcatttt atggtgagtg atgacgaagc acggcagatt atgcgtgagt
    34981 tgctgacact ggtgtcggga tatctggtgc ctaaactggc gcgagaaatt ggcggcgaac
    35041 ccagcaaaac accgctggat ctccagctac gccagcagta actgattaaa cagggaagtt
    35101 taatctttgc ccgttgcata acctttaatt attcata
//
LOCUS       DAHHTG010000039        35109 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.039, whole genome shotgun sequence.
ACCESSION   DAHHTG010000039 DAHHTG010000000
VERSION     DAHHTG010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 35109)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35109)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35109
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.039"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            161..1819
                     /locus_tag="NBI74_001879"
     CDS             161..1819
                     /locus_tag="NBI74_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent endonuclease"
                     /protein_id="HCD1932229.1"
                     /translation="MILERVEIVGFRGINRLSLMLEQNNVLIGENAWGKSSLLDALTL
                     LLSPESDLYHFERDDFWFPPGDINGREHHLHIILTFRESLPGRHRVRRYRPLEACWTP
                     CTDGYHRIFYRLEGESAEDGSVMTLRSFLDKDGHPIDVEDINDQARHLVRLMPVLRLR
                     DARFMRRIRNGTVPNVPNVEVTARQLDFLARELSSHPQNLSDGQIRQGLSAMVQLLEH
                     YFSEQGAGQARYRLMRRRASNEQRSWRYLDIINRMIDRPGGRSYRVILLGLFATLLQA
                     KGTLRLDKDARPLLLIEDPETRLHPIMLSVAWHLLNLLPLQRIATTNSGELLSLTPVE
                     HVCRLVRESSRVAAWRLGPSGLSTEDSRRISFHIRFNRPSSLFARCWLLVEGETETWV
                     INELARQCGHHFDAEGIKVIEFAQSGLKPLVKFARRMGIEWHVLVDGDEAGKKYAATV
                     RSLLNNDREAEREHLTALPALDMEHFMYRQGFSDVFHRVAQIPENIPMNLRKIISKAI
                     HRSSKPDLAIEVAMEAGRRGVDSVPTLLKKMFSRVLWLARGRAD"
     gene            complement(1816..2772)
                     /locus_tag="NBI74_001880"
     CDS             complement(1816..2772)
                     /locus_tag="NBI74_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001351020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF535 domain-containing protein"
                     /protein_id="HCD1932230.1"
                     /translation="MNMSQLTERTFTPSESLSSLSLFLSLARGQCRPGKFWHRRSFRQ
                     KFLLRSLIMPRLSVEWMNELSHWPNLNVLLTRQPRLPVRLHRPYLAANLSRKQLLEAL
                     RYHYALLRGCMSAEEFSLYLNTPGLQLAKLEGKNGEQFTLELTMMISMDKEGDSTILF
                     RNSEGIPLAELTFTLCEYQGKRTMFIGGLQGAKWEIPHQEIQNATKACHGLFPKRLVM
                     EAACLFAQRLQVEQIIAVSNETHIYRSLRYRDKEGKIHADYNAFWESVGGVCDAERHY
                     RLPAQIARKEIAEIASKKRAEYRRRYEMLDAIQPQMATMFCS"
     gene            2923..4038
                     /gene="macA"
                     /locus_tag="NBI74_001881"
     CDS             2923..4038
                     /gene="macA"
                     /locus_tag="NBI74_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide transporter subunit MacA"
                     /protein_id="HCD1932231.1"
                     /translation="MKKRKTVKKRYVIAMVIVIAGLITLWRILNAPVPTYQTLIVRPG
                     DLQQSVLATGKLDALRKVDVGAQVSGQLKTLSVAIGDKVKKDQLLGVIDPEQAENQIK
                     EVEATLMELRAQRQQAEAELKLARVTYSRQQRLAQTQAVSQQDLDNAATEMAVKQAQI
                     GTIDAQIKRNQASLDTAKTNLDYTRIVAPMAGEVTQITTLQGQTVIAAQQAPNILTLA
                     DMSTMLVKAQVSEADVIHLKPGQKAWFTVLGDPLTRYEGQIKDVLPTPEKINDAIFYY
                     ARFEVPNPNGLLRLDMTAQVHIQLTDVKNVLTIPLSALGDPVGDNRYKVKLLRNGETR
                     EREVTIGARNDTDVEIVKGLEAGDEVVIGEAKPGAAQ"
     gene            4035..5981
                     /gene="macB"
                     /locus_tag="NBI74_001882"
     CDS             4035..5981
                     /gene="macB"
                     /locus_tag="NBI74_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide ABC transporter ATP-binding
                     protein/permease MacB"
                     /protein_id="HCD1932232.1"
                     /translation="MTPLLELKDIRRSYPAGDEQVEVLKGISLDIYAGEMVAIVGASG
                     SGKSTLMNILGCLDKATSGTYRVAGQDVATLDADALAQLRREHFGFIFQRYHLLSHLT
                     AEQNVEVPAVYAGLEWKQRLLRAQELLQRLGLEDRTEYYPAQLSGGQQQRVSIARALM
                     NGGQVILADEPTGALDSHSGEEVMAILHQLRDRGHTVIIVTHDPQVAAQAERVIEIRD
                     GEIVRNPPAIEKVNATGGTEPVVNTASGWRQFVSGFNEALTMAWRALAANKMRTLLTM
                     LGIIIGIASVVSIVVVGDAAKQMVLADIRSIGTNTIDVYPGKDFGDDDPQYQQALKYD
                     DLIAIQKQPWVASATPAVSQNLRLRYNNVDVAASANGVSGDYFNVYGMTFSEGNTFNQ
                     EQLNGRAQVVVLDSNTRRQLFPHKADVVGEVILVGNMPARVIGVAEEKQSMFGSSKVL
                     RVWLPYSTMSGRVMGQSWLNSITVRVKEGFDSAEAEQQLTRLLSLRHGKKDFFTWNMD
                     GVLKTVEKTTRTLQLFLTLVAVISLVVGGIGVMNIMLVSVTERTREIGIRMAVGARAS
                     DVLQQFLIEAVLVCLVGGALGITLSLLIAFTLQLFLPGWEIGFSPLALLLAFLCSTVT
                     GILFGWLPARNAARLDPVDALARE"
     gene            complement(6054..6278)
                     /gene="cspD"
                     /locus_tag="NBI74_001883"
     CDS             complement(6054..6278)
                     /gene="cspD"
                     /locus_tag="NBI74_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspD"
                     /protein_id="HCD1932233.1"
                     /translation="MEKGTVKWFNNAKGFGFICPEGGGEDIFAHYSTIQMDGYRTLKA
                     GQSVQFDVHQGPKGNHASVIVPVEVEAAVA"
     gene            6601..6921
                     /gene="clpS"
                     /locus_tag="NBI74_001884"
     CDS             6601..6921
                     /gene="clpS"
                     /locus_tag="NBI74_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HCD1932234.1"
                     /translation="MGKTNDWLDFDQLAEEKVRDALKPPSMYKVILVNDDYTPMDFVI
                     DVLQKFFSYDVERATQLMLAVHYQGKAICGVFTAEVAETKVAMVNKYARENEHPLLCT
                     LEKA"
     gene            6952..9228
                     /gene="clpA"
                     /locus_tag="NBI74_001885"
     CDS             6952..9228
                     /gene="clpA"
                     /locus_tag="NBI74_001885"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HCD1932235.1"
                     /translation="MLNQELELSLNMAFARAREHRHEFMTVEHLLLALLSNPSAREAL
                     EACSVDLVALRQELEAFIEQTTPVLPASEEERDTQPTLSFQRVLQRAVFHVQSSGRNE
                     VTGANVLVAIFSEQESQAAYLLRKHEVSRLDVVNFISHGTRKDEPTQSSDPGSQPNSE
                     EQAGGEERMENFTTNLNQLARVGGIDPLIGREKELERAIQVLCRRRKNNPLLVGESGV
                     GKTAIAEGLAWRIVQGDVPEVMADCTIYSLDIGSLLAGTKYRGDFEKRFKALLKQLEQ
                     DTNSILFIDEIHTIIGAGAASGGQVDAANLIKPLLSSGKIRVIGSTTYQEFSNIFEKD
                     RALARRFQKIDITEPSIEETVQIINGLKPKYEAHHDVRYTAKAVRAAVELAVKYINDR
                     HLPDKAIDVIDEAGARARLMPVSKRKKTVNVADIESVVARIARIPEKSVSQSDRDTLK
                     NLGDRLKMLVFGQDKAIEALTEAIKMARAGLGHEHKPVGSFLFAGPTGVGKTEVTVQL
                     SKALGIELLRFDMSEYMERHTVSRLIGAPPGYVGFDQGGLLTDAVIKHPHAVLLLDEI
                     EKAHPDVFNILLQVMDNGTLTDNNGRKADFRNVVLVMTTNAGVRETERKSIGLIHQDN
                     STDAMEEIKKIFTPEFRNRLDNIIWFDHLSTDVIHQVVDKFIVELQVQLDQKGVSLEV
                     SQEARNWLAEKGYDRAMGARPMARVIQDNLKKPLANELLFGSLVDGGQVTVALDKEKN
                     ELTYGFQSAQKHKAEAAH"
     gene            complement(9572..9659)
                     /locus_tag="NBI74_001886"
     tRNA            complement(9572..9659)
                     /locus_tag="NBI74_001886"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9623..9625),aa:Ser,seq:gga)
     gene            complement(9913..10131)
                     /gene="infA"
                     /locus_tag="NBI74_001887"
     CDS             complement(9913..10131)
                     /gene="infA"
                     /locus_tag="NBI74_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HCD1932236.1"
                     /translation="MAKEDNIEMQGTVLETLPNTMFRVELENGHVVTAHISGKMRKNY
                     IRILTGDKVTVELTPYDLSKGRIVFRSR"
     gene            complement(10416..11120)
                     /gene="aat"
                     /locus_tag="NBI74_001888"
     CDS             complement(10416..11120)
                     /gene="aat"
                     /locus_tag="NBI74_001888"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HCD1932237.1"
                     /translation="MRLVQLSRHSIAFPSPEGALREPNGLLALGGDLSPARLLMAYQR
                     GIFPWFSPGDPILWWSPDPRAVLWPESLHISRSMKRFHKRSPYRVTMNYAFGQVIEGC
                     ASDREEGTWITRGVVEAYHRLHELGHAHSIEVWREDELVGGMYGVAQGTLFCGESMFS
                     RMENASKTALLVFCDEFIRHGGKLIDCQVLNDHTASLGACEIPRRDYLNYLNQMRLGR
                     LSNNFWVPRCLFSPQE"
     gene            complement(11162..12883)
                     /gene="cydC"
                     /locus_tag="NBI74_001889"
     CDS             complement(11162..12883)
                     /gene="cydC"
                     /locus_tag="NBI74_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter ATP-binding
                     protein/permease CydC"
                     /protein_id="HCD1932238.1"
                     /translation="MRALLPYLALYKRHKWMLSLGIVLAIVTLLASIGLLTLSGWFLS
                     ASAVAGVAGLYSFNYMLPAAGVRGAAITRTAGRYFERLVSHDATFRVLQHLRIYTFSK
                     LLPLSPAGLARYRQGELLNRVVADVDTLDHLYLRVISPLVGAFVVIMVVTIGLSFLDF
                     TLAFTLGGIMLLTLFLMPPLFYRAGKSTGQNLTHLRGQYRQQLTAWLQGQAELTIFGA
                     SDRYRTQLENTEIQWLEAQRRQSELTALSQAIMLLIGALAVILMLWMASGGVGGNAQP
                     GALIALFVFCALAAFEALAPVTGAFQHLGQVIASAVRITDLTDQKPKVTFPDTQTRVA
                     DRVSLTLRDVQFTYPEQSQQALKGIYLQVNAGEHIAILGRTGCGKSTLLQLLTRAWDP
                     QQGEILLNDSPIASLNEAALRQTISVVPQRVHLFSATLRDNLLLASPGSSDEALAEIL
                     RRVGLEKLLEDAGLNSWLGEGGRQLSGGELRRLAIARALLHDAPLVLLDEPTEGLDAT
                     TESQILELLAEMMCEKTVLMVTHRLRGLSRFQQIIVMDNGQIIEQGTHAELLARQGRY
                     YQFKQGL"
     gene            complement(12884..14650)
                     /gene="cydD"
                     /locus_tag="NBI74_001890"
     CDS             complement(12884..14650)
                     /gene="cydD"
                     /locus_tag="NBI74_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter
                     permease/ATP-binding protein CydD"
                     /protein_id="HCD1932239.1"
                     /translation="MNKSRQKELTRWLKQQSVISQRWLNISRLLGFVSGILIIAQAWF
                     MARILQHMIMENIPREALLLPFTLLFLTFVLRAWVVWLRERVGYHAGQHIRFAIRRQV
                     LDRLQQAGPAWIQGKPAGSWATLVLEQIDDMHDYYARYLPQMALAVSVPLLIVVAIFP
                     SNWAAALILLGTAPLIPLFMALVGMGAADANRRNFLALARLSGHFLDRLRGMETLRIF
                     GRGEAEIESIRSASEDFRQRTMEVLRLAFLSSGILEFFTSLSIALVAVYFGFSYLGEL
                     DFGHYDTGVTLAAGFLALILAPEFFQPLRDLGTFYHAKAQAVGAADSLKTFMETPLAH
                     PQRGEAELASTDPVTIEAEDLFITSPEGKTLAGPLNFTLPAGQRAVLVGRSGSGKSSL
                     LNALSGFLSYQGSLRINGIELRDLSPESWRKHLSWVGQNPQLPAATLRDNVLLARPDA
                     SEQELQTALDNAWVSEFLPLLPQGVDTPVGDQAARLSVGQAQRVAVARALLNPCSLLL
                     LDEPAASLDAHSEQRVMEALNAASLRQTTLMVTHQLEDLADWDVIWVMQDGQIIEQGR
                     YAELSVAGGPFATLLAHRQEEI"
     gene            complement(14773..15738)
                     /gene="trxB"
                     /locus_tag="NBI74_001891"
     CDS             complement(14773..15738)
                     /gene="trxB"
                     /locus_tag="NBI74_001891"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HCD1932240.1"
                     /translation="MGTTKHSKLLILGSGPAGYTAAVYAARANLQPVLITGMEKGGQL
                     TTTTEVENWPGDPNDLTGPLLMERMHEHATKFETEIIFDHINKVDLQNRPFRLTGDSG
                     EYTCDALIIATGASARYLGLPSEEAFKGRGVSACATCDGFFYRNQKVAVIGGGNTAVE
                     EALYLSNIASEVHLIHRRDGFRAEKILIKRLMDKVENGNIILHTNRTLEEVTGDQMGV
                     TGVRLRDTQNTDNIESLDVAGLFVAIGHSPNTAIFEGQLELENGYIKVQSGIHGNATQ
                     TSIPGVFAAGDVMDHIYRQAITSAGTGCMAALDAERYLDGLADAK"
     gene            16282..16776
                     /gene="lrp"
                     /locus_tag="NBI74_001892"
     CDS             16282..16776
                     /gene="lrp"
                     /locus_tag="NBI74_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001591745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-responsive transcriptional regulator
                     Lrp"
                     /protein_id="HCD1932241.1"
                     /translation="MVDSKKRPGKDLDRIDRNILNELQKDGRISNVELSKRVGLSPTP
                     CLERVRRLERQGFIQGYTALLNPHYLDASLLVFVEITLNRGAPDVFEQFNTAVQKLEE
                     IQECHLVSGDFDYLLKTRVPDMSAYRKLLGETLLRLPGVNDTRTYVVMEEVKQSNRLV
                     IKTR"
     gene            16911..21017
                     /gene="ftsK"
                     /locus_tag="NBI74_001893"
     CDS             16911..21017
                     /gene="ftsK"
                     /locus_tag="NBI74_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HCD1932242.1"
                     /translation="MSQEYTEDKEVTLTKLSSGRRLLEALLILIVLFAVWLMAALLSF
                     NPSDPSWSQTAWHEPIHNLGGMPGAWLADTLFFIFGVMAYTIPVIIVGGCWFAWRHQS
                     SDEYIDYFAVSLRIIGVLALILTSCGLAAINADDIWYFASGGVIGSLLSTTLQPLLHS
                     SGGTIALLCVWAAGLTLFTGWSWVTIAEKLGGWILNILTFASNRTRRDDTWVDEDEYE
                     DDEEYEDENHGKQHESRRARILRGALARRKRLAEKFINPMGRQTDAALFSGKRMDDDE
                     EITYTVRGVAADPDDVLFSGNRATQPEYDEYDPLLNGAPITEPVAVAAAATTATQSWA
                     APVEPVTQTPPVASVDVPPAQSTVAWQPVPGPQTGEPVIAPAPEGYPQQPQYAQPAVQ
                     YNEPLQQPVQPQQPYYAPAAEQPAQQPYYAPAAEQPVQQPYYATAAEQPAQQPYYAPA
                     PEQAVAGNAWQAEEQQSTFAPQSTYQTEQTYQQPAAQEPLYQQPQPVEQQPVVEPEPV
                     VEETKPTRPPLYYFEEVEEKRAREREQLAAWYQPIPEPVKEPEPIKSSLKAPSVAAVP
                     PVEAAAAVSPLASGVKKATLATGAAATVAAPVFSLANSGGPRPQVKEGIGPQLPRPKR
                     IRVPTRRELASYGIKLPSQRAAEEKAREAQRNQYDSGDQYNDDEIDAMQQDELARQFA
                     QTQQQRYGEQYQHDVPVNTEDADAAAEAELARQFAQTQQQRYSGEQPAGANPFSLDDF
                     EFSPMKALLDDGPHEPLFTPIVEPVQQPQQPVAPQQQYQQPQQPVAPQPQYQQPQQPV
                     APQQQYQQPQQPVAQQPQYQQPQQPVTQQPQYQQPQQPVVPQPQYQQPQQPVAPQPQD
                     TLLHPLLMRNGDSRPLHKPTTPLPSLDLLTPPPSEVEPVDTFALEQMARLVEARLADF
                     RIKADVVNYSPGPVITRFELNLAPGVKAARISNLSRDLARSLSTVAVRVVEVIPGKPY
                     VGLELPNKKRQTVYLREVLDNAKFRDNPSPLTVVLGKDIAGEPVVADLAKMPHLLVAG
                     TTGSGKSVGVNAMILSMLYKAQPEDVRFIMIDPKMLELSVYEGIPHLLTEVVTDMKDA
                     ANALRWCVNEMERRYKLMSALGVRNLAGYNEKIAEADRMMRPIPDPYWKPGDSMDAQH
                     PVLKKEPYIVVLVDEFADLMMTVGKKVEELIARLAQKARAAGIHLVLATQRPSVDVIT
                     GLIKANIPTRIAFTVSSKIDSRTILDQAGAESLLGMGDMLYSGPNSTLPVRVHGAFVR
                     DQEVHAVVQDWKARGRPQYVDGITSDSESEGGAGGFDGAEELDPLFDQAVQFVTEKRK
                     ASISGVQRQFRIGYNRAARIIEQMEAQGIVSEQGHNGNREVLAPPPFD"
     gene            21176..21787
                     /gene="lolA"
                     /locus_tag="NBI74_001894"
     CDS             21176..21787
                     /gene="lolA"
                     /locus_tag="NBI74_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010354356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HCD1932243.1"
                     /translation="MKKIAITCALLSSLVASSVWADAASDLKSRLDKVSSFHASFTQK
                     VTDGSGAAVQEGQGDLWVKRPNLFNWHMTQPDESILVSDGKTLWFYNPFVEQATATWL
                     KDATGNTPFMLIARNQSSDWQQYNIKQNGDDFVLTPKASNGNLKQFTINVGRDGTIHQ
                     FSAVEQDDQRSSYQLKSQQNGAVDAAKFTFTPPQGVTVDDQRK"
     gene            21798..23141
                     /gene="rarA"
                     /locus_tag="NBI74_001895"
                     /gene_synonym="mgsA"
     CDS             21798..23141
                     /gene="rarA"
                     /locus_tag="NBI74_001895"
                     /gene_synonym="mgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein
                     RarA"
                     /protein_id="HCD1932244.1"
                     /translation="MSNLSLDFSDNTFQPLAARMRPENLAQYIGQQHLLAVGKPLPRA
                     IEAGHLHSMILWGPPGTGKTTLAEVIARYANADVERISAVTSGVKEIREAIERARQNR
                     NAGRRTILFVDEVHRFNKSQQDAFLPHIEDGTITFIGATTENPSFELNSALLSRARVY
                     LLKSLSTEDIEQVLTQAMEDKTRGYGGQDIVLPDETRRAIAELVNGDARRALNTLEMM
                     ADMAEVDDSGKRVLKPELLTEIAGERSARFDNKGDRFYDLISALHKSVRGSAPDAALY
                     WYARIITAGGDPLYVARRCLAIASEDVGNADPRAMQVAIAAWDCFTRVGPAEGERAIA
                     QAIVYLACAPKSNAVYTAFKAALADARERPDYDVPVHLRNAPTKLMKEMGYGQEYRYA
                     HDEANAYAAGEVYFPPEIAQTRYYFPTNRGLEGKIGEKLAWLAEQDQNSPIKRYR"
     gene            23232..24524
                     /gene="serS"
                     /locus_tag="NBI74_001896"
     CDS             23232..24524
                     /gene="serS"
                     /locus_tag="NBI74_001896"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HCD1932245.1"
                     /translation="MLDPNLLRNEPDAVAEKLARRGFKLDVDKLGALEERRKVLQVKT
                     ENLQAERNSRSKSIGQAKARGEDIEPLRLEVNKLGEELDAAKAELDALQAEIRDIALT
                     IPNLPADEVPVGKDENDNVEVSRWGTPREFDFEVRDHVTLGEMHSGLDFAAAVKLTGS
                     RFVVMKGQIARMHRALSQFMLDLHTEQHGYSENYVPYLVNQDTLYGTGQLPKFAGDLF
                     HTRPLEEEADTSNYALIPTAEVPLTNLVRGEIIDEDDLPIKMTAHTPCFRSEAGSYGR
                     DTRGLIRMHQFDKVEMVQIVRPEDSMAALEEMTGHAEKVLQLLGLPYRKIILCTGDMG
                     FGACKTYDLEVWIPAQNTYREISSCSNVWDFQARRMQARCRSKSDKKTRLVHTLNGSG
                     LAVGRTLVAVMENYQQADGRIEVPEVLRPYMNGLEYIG"
     gene            24763..27207
                     /gene="dmsA"
                     /locus_tag="NBI74_001897"
     CDS             24763..27207
                     /gene="dmsA"
                     /locus_tag="NBI74_001897"
                     /EC_number="1.8.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415414.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit A"
                     /protein_id="HCD1932246.1"
                     /translation="MKTKIPDAVLAAEVSRRGLVKTTAIGGLAMASSALTLPFSRIAH
                     AVDSAIPTKSDEKVIWSACTVNCGSRCPLRMHVVDGEIKYVETDNTGDDNYDGLHQVR
                     ACLRGRSMRRRVYNPDRLKYPMKRVGARGEGKFERISWEEAYDIIATNMQRLIKEYGN
                     ESIYLNYGTGTLGGTMTRSWPPGNTLVARLMNCCGGYLNHYGDYSSAQIAEGLNYTYG
                     GWADGNSPSDIENSKLVVLFGNNPGETRMSGGGVTYYLEQARQKSNARMIIIDPRYTD
                     TGAGREDEWIPIRPGTDAALVNGLAYVMITENLVDQAFLDKYCVGYDEKTLPASAPKN
                     GHYKAYILGEGPDGVAKTPEWASQITGVPADKIIKLAREIGSTKPAFISQGWGPQRHA
                     NGEIATRAISMLAILTGNVGINGGNSGAREGSYSLPFVRMPTLENPIQTSISMFMWTD
                     AIERGPEMTALRDGVRGKDKLDVPIKMIWNYAGNCLINQHSEINRTHEILQDDKKCEL
                     IVVIDCHMTSSAKYADILLPDCTASEQMDFALDASCGNMSYVIFNDQVIKPRFECKTI
                     YEMTSELAKRLGVEQQFTEGRTQEEWMRHLYAQSREAIPELPTFEEFRKQGIFKKRDP
                     QGHHVAYKAFREDPQANPLTTPSGKIEIYSQALADIAATWELPEGDVIDPLPIYTPGF
                     ESYQDPLNKQYPLQLTGFHYKSRVHSTYGNVDVLKAACRQEMWINPLDAQKRGINNGD
                     KVRIFNDRGEVHIEAKVTPRMMPGVVALGEGAWYDPDTKRVDKGGCINVLTTQRPSPL
                     AKGNPSHTNLVQVEKV"
     gene            27218..27835
                     /gene="dmsB"
                     /locus_tag="NBI74_001898"
     CDS             27218..27835
                     /gene="dmsB"
                     /locus_tag="NBI74_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit B"
                     /protein_id="HCD1932247.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDYKDLTPEVSFRRIYEYAG
                     GDWQEDNGVWHQNVFAYYLSISCNHCEDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNETKGHMTKCDGCYDRVAEGKKPICVESCPLRALDFGPIDELRK
                     KHGDLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPKEV"
     gene            27837..28700
                     /gene="dmsC"
                     /locus_tag="NBI74_001899"
     CDS             27837..28700
                     /gene="dmsC"
                     /locus_tag="NBI74_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit
                     DmsC"
                     /protein_id="HCD1932248.1"
                     /translation="MGSGWHEWPLMIFTVFGQCVAGGFIVLALALLKGDLRAEAQQRV
                     IACMLGLWVLMGIGFISSMLHLGSPMRAFNSLNRVGASALSNEIASGSIFFAVGGIGW
                     LLAMLKKLSPALRTLWLVVTMVLGVVFVWMMVRVYNSIDTVPTWYSIWTPMGFFLTMF
                     MGGPLLGYLLLSLAGVDGWAMRLLPAISVLALVVSGVMSVMQGAELATIHSSVQQAAA
                     LVPDYGALMSWRIVLLAVALCVWIAPQLKGYQPAVPLLSVSFILLLAGELIGRGVFYG
                     LHMTVGMAVAN"
     gene            complement(28736..29362)
                     /locus_tag="NBI74_001900"
     CDS             complement(28736..29362)
                     /locus_tag="NBI74_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HCD1932249.1"
                     /translation="MTKPYVRLDKNDAAVLLVDHQAGLLSLVRDIEPDKFKNNVLALG
                     DLAKYFNLPTILTTSFETGPNGPLVPELKAQFPDAPYIARPGNINAWDNEDFVKAVKA
                     TGKKQLIIAGVVTEVCVAFPALSAIEEGFDVFVVTDASGTFNEITRHSAWDRMSQAGA
                     QLMTWFGVACELHRDWRNDIEGLATLFSNHIPDYRNLMTSYDTLTKQK"
     gene            29676..30824
                     /locus_tag="NBI74_001901"
     CDS             29676..30824
                     /locus_tag="NBI74_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCD1932250.1"
                     /translation="MSTYTRPVMLLLSGLLLLTLAIAVLNTLVPLWLAQEHMSTWQVG
                     VVSSSYFTGNLVGTLLTGYVIKRIGFNRSYYLASFIFAAGCAGLGLMIGFWSWLAWRF
                     VAGIGCAMIWVVVESALMCSGTSRNRGRLLAAYMMVYYVGTFLGQLLVSKVSTELMSV
                     LPWVTGLTLAGILPLLFTRVLNQQTENHDSTSITSMLKLRQARLGVNGCIISGIVLGS
                     LYGLMPLYLNHKGVSNASIGFWMAVLVSAGILGQWPIGRLADKFGRLLVLRVQVFVVI
                     LGSIAMLSQAAMAPALFILGAAGFTLYPVAMAWACEKVEHHQLVAMNQALLLSYTVGS
                     LLGPSFTAMLMQNFSDNLLFIMIASVSFIYLLMLLRNAGHTPKPVAHV"
     gene            complement(30921..31661)
                     /gene="pflA"
                     /locus_tag="NBI74_001902"
     CDS             complement(30921..31661)
                     /gene="pflA"
                     /locus_tag="NBI74_001902"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase 1-activating protein"
                     /protein_id="HCD1932251.1"
                     /translation="MSVIGRIHSFESCGTVDGPGIRFITFFQGCLMRCLYCHNRDTWD
                     THGGKEVTVEDLMKEVVTYRHFMNASGGGVTASGGEAILQAEFVRDWFRACKKEGIHT
                     CLDTNGFVRRYDPVIDELLEVTDLVMLDLKQMNDEIHQNLVGVSNHRTLEFAKYLANK
                     NVKVWIRYVVVPGWSDDDDSAHRLGEFTRDMGNVEKIELLPYHELGKHKWVAMGEEYK
                     LDGVKPPKKETMERVKGILEQYGHKVMF"
     gene            complement(31853..34135)
                     /gene="pflB"
                     /locus_tag="NBI74_001903"
     CDS             complement(31853..34135)
                     /gene="pflB"
                     /locus_tag="NBI74_001903"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HCD1932252.1"
                     /translation="MSELNEKLATAWEGFTKGDWQNEVNVRDFIQKNYTPYEGDESFL
                     AGATEATTTLWDKVMEGVKLENRTHAPVDFDTAVASTITSHDAGYINKQLEKIVGLQT
                     EAPLKRALIPFGGIKMIEGSCKAYNRELDPMIKKIFTEYRKTHNQGVFDVYTPDILRC
                     RKSGVLTGLPDAYGRGRIIGDYRRVALYGIDYLMKDKLAQFTSLQADLENGVNLEQTI
                     RLREEIAEQHRALGQMKEMAAKYGYDISGPATNAQEAIQWTYFGYLAAVKSQNGAAMS
                     FGRTSTFLDVYIERDLKAGKITEQEAQEMVDHLVMKLRMVRFLRTPEYDELFSGDPIW
                     ATESIGGMGLDGRTLVTKNSFRFLNTLYTMGPSPEPNMTILWSEKLPLNFKKFAAKVS
                     IDTSSLQYENDDLMRPDFNNDDYAIACCVSPMIVGKQMQFFGARANLAKTMLYAINGG
                     VDEKLKMQVGPKSEPIKGDVLNYDEVMERMDHFMDWLAKQYITALNIIHYMHDKYSYE
                     ASLMALHDRDVIRTMACGIAGLSVAADSLSAIKYAKVKPIRDEDGLAIDFEIEGEYPQ
                     FGNNDPRVDDLAVDLVERFMKKIQKLHTYRDAIPTQSVLTITSNVVYGKKTGNTPDGR
                     RAGAPFGPGANPMHGRDQKGAVASLTSVAKLPFAYAKDGISYTFSIVPNALGKDDEVR
                     KTNLAGLMDGYFHHEASIEGGQHLNVNVMNREMLLDAMENPEKYPQLTIRVSGYAVRF
                     NSLTKEQQQDVITRTFTQSM"
     gene            complement(34190..35047)
                     /gene="focA"
                     /locus_tag="NBI74_001904"
     CDS             complement(34190..35047)
                     /gene="focA"
                     /locus_tag="NBI74_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter FocA"
                     /protein_id="HCD1932253.1"
                     /translation="MKADNPFDLLLPAAMAKVAEEAGVYKATKHPLKTFYLAITAGVF
                     ISIAFVFYITATTGTGTMPFGMAKLIGGICFSLGLILCVVCGADLFTSTVLIVVAKAS
                     GRITWGQLAKNWLNVYFGNLVGALLFVLLMWLSGEYMTANGQWGLNVLQTADHKVHHT
                     FIEAVCLGILANLMVCLAVWMSYSGRSLMDKAFIMVLPVAMFVASGFEHSIANMFMIP
                     MGIVIRDFASPEFWTAVGSAPENFSHLTVMNFITDNLIPVTIGNIIGGGLLVGLTYWV
                     IYLRENDHH"
BASE COUNT         8644 a         8615 c         9410 g         8440 t
ORIGIN      
        1 tgttgttttt gcaggcttat cagcaagagg gagtataacg cgattattcg ctcatttttc
       61 agacatttgc catgcttaaa tgtgatgcca tcacgtatta gcaggccttt cccgttatac
      121 tgccagcgta aagaataagt cacatatttc tggaggggat atgattcttg agcgcgttga
      181 aattgtgggt tttcgcggta tcaaccgttt gtcgttgatg ctggaacaaa acaacgtcct
      241 gattggggag aacgcgtggg gtaaatccag cttgctggac gccttaaccc tgctgctatc
      301 gccagaatca gatctctacc attttgagcg cgatgatttc tggttcccgc cgggagatat
      361 caacgggcga gaacatcatc tgcatattat tttgaccttc cgcgaatcgc tgccaggccg
      421 acatcgggtt cgccgctatc ggccgctgga agcgtgctgg acgccatgca ccgatggtta
      481 tcaccgtatt ttttatcgtc tggaagggga gagtgcggaa gacggcagcg tgatgacact
      541 gcgcagtttt ctcgataaag acggacatcc gattgatgtt gaggatatta acgatcaggc
      601 tcgtcatctg gtgcgtttaa tgccggtgct gcgcttgcgt gatgcccgtt ttatgcgccg
      661 tattcgtaac ggcacggtgc caaatgtccc caatgtggaa gtcaccgcgc gccagctcga
      721 tttcctcgcc cgtgagttgt cctcacatcc gcaaaatctc tctgatggac agattcgtca
      781 gggactttcc gcaatggtgc aactgcttga gcattatttt tctgagcagg gagctggaca
      841 ggcgcgatat cgtttaatgc ggcggcgagc cagcaatgag caacgaagct ggcgctatct
      901 ggatatcatc aaccggatga ttgaccgacc tggagggcgc tcgtatcggg ttattttgct
      961 cggcctgttt gctactttgt tgcaggcaaa aggcacattg cgactggata aagacgcccg
     1021 tccattgttg ctgatcgaag atccagaaac ccgtttgcac cccattatgc tttcagttgc
     1081 ctggcatctg ttgaaccttc tgccattgca gcgcatcgct actaccaact cgggagagtt
     1141 gctttcgtta acgccggtag agcatgtttg ccgactggta cgtgagtcct ctcgcgttgc
     1201 cgcctggcgt ctgggaccga gtggcttgag taccgaagat agccgacgca tctcctttca
     1261 cattcgtttt aaccgtccgt catcgctgtt tgcccgttgt tggctgctgg tggaagggga
     1321 aacggaaacc tgggttatca atgaactggc gcgtcagtgc ggacatcatt ttgatgccga
     1381 agggatcaag gtcattgagt ttgcccagtc cgggctaaag ccactggtta aatttgcccg
     1441 gcgaatgggg attgaatggc atgtactggt cgatggcgat gaagcaggga agaaatatgc
     1501 cgctacggta cgcagcctgt tgaataatga tcgggaagcc gaacgagaac atttaacggc
     1561 gttaccggcg ctggatatgg aacattttat gtatcgccag ggattttccg atgtgttcca
     1621 ccgcgtggcg caaatcccgg aaaatatacc gatgaatctg cgcaaaatta tctcgaaagc
     1681 gatccatcgc tcttccaaac ccgatcttgc cattgaagtg gcaatggagg ccggacgtcg
     1741 tggtgtggac tccgtaccga cgctgctgaa aaaaatgttc tcacgcgtgc tgtggctggc
     1801 gcgcggtcgg gccgactaac tacaaaacat cgtggccatt tgtggctgaa tagcgtcgag
     1861 catctcatag cgccgacggt attcagcccg ttttttactg gcgatttcgg caatctcttt
     1921 tcgtgctatc tgtgctggaa ggcggtaatg gcgttcagca tcacatacgc cgccaaccga
     1981 ttcccagaaa gcgttgtaat cggcatggat cttgccttct ttatcgcgat aacgcaggct
     2041 gcggtaaata tgcgtttcat tgctgacggc aataatctgc tctacctgca aacgttgggc
     2101 aaacagacag gccgcttcca tcacgaggcg tttgggaaat agcccgtggc aggctttcgt
     2161 cgcattctgg atttcctggt gtggaatttc ccattttgcg ccttgcagtc cgccaataaa
     2221 catcgtcctt ttcccctgat attcacacag ggtaaaagtg agctctgcca gaggaatacc
     2281 ttcgctgttg cggaacagga tggtgctgtc accttcttta tccattgaga tcatcatggt
     2341 cagctcaagc gtgaactgct cgccgttttt gccttccagc ttcgccagtt gcagcccggg
     2401 ggtattcaaa tataagctga attcttccgc cgacatacac ccgcggagta acgcataatg
     2461 gtaacgtaac gcctccagca attgcttacg gctaagattc gccgcaaggt aagggcgatg
     2521 tagacgtaca ggcagtcgcg gctggcgcgt taacaacaca ttgagattag gccagtggga
     2581 aagttcgttc atccactcaa cgcttaaacg cggcataatc aacgagcgca gcaaaaattt
     2641 ctggcgaaaa ctacggcgat gccagaattt acccggccga cactgtcctc gtgccagact
     2701 aagaaaaagt gacaggctgc tgagagattc agatggcgta aaggtccgtt cagttagctg
     2761 cgacatattc atgaaatcaa tggttataca tgacgtcgat ttaaccattg cgtatcttaa
     2821 ccaaacatca atagtgtgat tattaacgta aattttaggg ttttgttgat atttcgttga
     2881 agttaatgac ccggattggc atatggagta ttcagaatat ttatgaaaaa gcggaaaacc
     2941 gtgaagaagc gttacgttat tgcgatggtg atagtcatcg ccgggctgat tacgttatgg
     3001 agaattctta acgcacccgt gccgacttat cagacgctga ttgtgcgccc cggtgattta
     3061 cagcaaagcg tgctggcgac cggaaagctg gacgcgctgc gtaaggttga cgtgggcgcg
     3121 caggtcagcg gtcagttgaa aacgctgtcg gtggcgattg gcgataaagt aaaaaaagac
     3181 cagcttttag gggttattga tcctgaacag gctgaaaacc agatcaagga ggtggaagca
     3241 acgctgatgg agctacgcgc gcagcggcag caggcggaag cggagctgaa actggcgcgg
     3301 gtgacgtatt cccgtcagca acgtcttgca caaacgcaag cggtttcaca gcaggatctc
     3361 gataacgccg cgacggagat ggctgtgaaa caggcgcaaa ttggcaccat tgacgcgcaa
     3421 atcaagcgca atcaggcctc tctcgatacg gctaaaacca atctcgatta cacccgcatc
     3481 gttgccccga tggccgggga agtcacgcaa atcaccactc tgcaaggcca gacggtgatt
     3541 gccgcacaac aagcaccgaa cattctgacg ctggcagata tgagcaccat gctggtaaaa
     3601 gcgcaggttt ctgaagcgga tgtaatccac ctgaagccgg ggcaaaaagc ctggtttacg
     3661 gtgcttggcg atccactgac gcgctacgag gggcaaatca aggatgtgct accgacgccg
     3721 gaaaagatta acgacgctat tttctattac gcccgttttg aagtacccaa ccccaatggt
     3781 ttactgcggc tggatatgac cgcgcaagtg catattcagc tcaccgatgt gaaaaatgtg
     3841 ctgacgatcc ctctgtcggc gttaggcgat ccggttggcg ataatcgtta taaagtcaaa
     3901 ttgttgcgta atggtgaaac acgcgagcgt gaagtgacga ttggcgcacg taacgatacc
     3961 gatgttgaga ttgtcaaagg gctggaagcg ggcgatgaag tggtgattgg tgaggccaaa
     4021 ccaggagctg cgcaatgacg cctttgctcg aattaaagga tattcgtcgc agctatcctg
     4081 ccggtgatga gcaggttgag gtgctgaagg gcatcagcct cgatatttat gccggtgaga
     4141 tggtagcgat tgtcggtgct tcgggttctg gtaaatcgac cctgatgaat attctcggct
     4201 gcctggataa ggccaccagc ggcacctatc gcgtcgccgg tcaggatgtt gccacgctgg
     4261 acgccgatgc gctggcgcaa ctgcgccgcg agcatttcgg ctttattttc cagcgttacc
     4321 atttgctttc gcatttaacc gccgagcaga acgttgaagt acccgccgtc tatgccggtc
     4381 ttgagtggaa acagcggctg cttcgcgccc aggagttact gcaacgactg gggctggaag
     4441 accgtacaga gtattacccg gcacagcttt cgggtggcca gcaacagcgc gtcagtatcg
     4501 cgcgggcatt gatgaacggc ggtcaggtaa ttcttgccga tgaaccgacc ggcgcactgg
     4561 acagccattc tggcgaagag gtgatggcga tcctgcatca gctgcgcgat cgcgggcata
     4621 cggtgattat cgtcacacac gatccgcagg ttgctgctca ggccgagcgg gtgatcgaaa
     4681 ttcgcgacgg cgaaattgtg cgcaatcctc ccgccattga aaaagtgaat gctactggcg
     4741 gaacggaacc tgttgtcaac acggcgtctg gctggcggca gtttgtcagc ggttttaacg
     4801 aggcgctgac gatggcatgg cgggcgctgg cagcgaataa aatgcgtact ttactgacca
     4861 tgctggggat tattatcggt attgcgtcgg tggtttccat tgtcgtggtg ggcgacgccg
     4921 ccaaacaaat ggtgctggcg gatattcgtt ctattggtac gaatactatt gatgtctatc
     4981 ccgggaaaga ttttggcgat gacgatccgc aatatcagca ggcgctgaag tacgacgact
     5041 taatcgccat ccaaaaacaa ccgtgggtcg cctcagccac acctgccgtc tcgcaaaacc
     5101 tgcgcctgcg ttataacaat gttgatgtgg ctgccagtgc caatggcgtg agcggcgatt
     5161 attttaatgt ctatggcatg actttcagtg aagggaacac ctttaatcag gagcagctga
     5221 acggtcgtgc gcaggtcgtg gttctcgaca gtaatactcg ccgccagctt ttcccccata
     5281 aagcagatgt ggttggcgag gtgattctgg ttggcaatat gcccgccaga gtcattggtg
     5341 tggcggaaga aaaacagtcg atgtttggta gcagtaaagt gctgcgtgtc tggctgccat
     5401 acagcacgat gtccgggcga gttatgggtc agtcgtggct gaactccata actgtcaggg
     5461 tgaaagaagg ctttgacagc gctgaggcgg aacagcaact cacgcgttta ctttcactgc
     5521 gccacggtaa gaaggatttc tttacctgga acatggatgg cgtcttgaaa accgttgaaa
     5581 agaccacacg tactttacaa ctgtttctga cgctggtggc ggtgatttcg ctggtggtgg
     5641 gcggtattgg tgtaatgaat attatgctgg tgtcagtgac cgagcggacg cgggaaattg
     5701 gcattcgcat ggctgtaggt gcgcgagcaa gcgatgtttt gcaacagttt ctgatcgaag
     5761 ccgtactggt ttgcctggtt ggtggcgctt tgggaataac actgtcactg ttaattgctt
     5821 tcaccttgca gcttttctta cccggctggg agattggttt ttcaccgtta gcgctgctgc
     5881 tggcgtttct ctgctcgacg gtcaccggga ttttatttgg ctggttaccc gcacgaaatg
     5941 cggcacgact ggatccagta gatgctctgg cacgggagta atttttgaga taaaaatgcc
     6001 agccgatcgg gctggcattt tgcctttagg atgtacacaa tgagacagaa gagctatgcg
     6061 actgccgctt ctacttcgac gggcacaata acactggcgt gattgccttt tggcccctgg
     6121 tggacatcaa actgaacgga ttgtccagct tttagcgttc tgtaaccatc catctgaatg
     6181 gtggaataat gagcgaaaat atcttcgccg ccgccttcag ggcagatgaa accaaaccct
     6241 ttggcattgt tgaaccactt aacagtaccc ttttccatgc ttcgacatcc ttcgcaaatc
     6301 ttatacaagt aagatggaat aaaccggggt cagagagggg gctgttcaaa acctcgccaa
     6361 ctctagaaat acaatttaga gaattagggc gagccgtcaa gcatttgaca ggggccaggg
     6421 ggcaggtatg aatcaaaaat ttgaagcagt taacgctatt gacaggaatg tgacagatgt
     6481 cgctgatgcc aacgatagat gatagttatc tatcatgtgg agtagattgg tcaggcaaat
     6541 aagctcttgt cagcggcagg gcgttctgcc gataaccgta accgaagatg ataactgaca
     6601 atgggtaaaa cgaacgactg gctggacttt gatcaactgg cggaagaaaa agttcgcgac
     6661 gcgctaaaac cgccatctat gtataaagtg atattagtca atgatgatta tactccgatg
     6721 gattttgtta ttgacgtgtt acaaaaattc ttttcttatg atgtagaacg tgcaacgcaa
     6781 ttgatgctcg ctgttcacta ccaggggaag gccatttgcg gagtctttac cgctgaggtt
     6841 gcagaaacca aagtggcgat ggtgaacaag tacgcgaggg agaatgagca tccattgctg
     6901 tgtacgctag aaaaagcctg aatgcaggca taaaaattgg gggaggtgcc tatgctcaat
     6961 caagaactgg aactcagttt aaatatggct ttcgccagag cgcgcgagca ccgtcatgag
     7021 tttatgaccg tcgagcactt gttactggcg ctgctcagta acccatctgc ccgggaggcg
     7081 ctggaagcgt gttctgtaga tttggttgcg ctccgtcagg aactggaagc ctttattgaa
     7141 caaaccacac ccgttctgcc tgccagtgaa gaggagcgcg acacacagcc gacgttgagt
     7201 tttcagcgtg tactgcaacg tgcggtcttc catgtccagt cctccggtcg caatgaggtt
     7261 accggtgcaa acgttctggt cgctatcttt agcgaacagg agtcgcaggc ggcatatctg
     7321 ttgcgcaaac atgaagtcag ccgtctcgat gtggtgaact ttatctctca tggcacgcgt
     7381 aaagacgagc cgacacagtc ttctgatcct ggcagccagc caaacagcga agaacaagct
     7441 ggtggggagg aacgtatgga gaatttcacg acgaacctga atcagcttgc gcgagtgggc
     7501 ggaatcgacc cactaattgg tcgtgagaag gagctggagc gtgctattca ggttctctgc
     7561 cgtcgccgta aaaacaaccc gctgctggtg ggggagtctg gtgtcggtaa aaccgcgatt
     7621 gcggaggggc ttgcatggcg aattgttcag ggcgatgtgc cggaagtgat ggctgactgt
     7681 acgatttact ctctcgatat cggttccctg ttagcgggca caaaatatcg cggcgacttt
     7741 gaaaaacgtt ttaaagcgtt gctcaagcag ctggagcagg acactaacag catcctgttt
     7801 attgatgaga tccacaccat tatcggtgcg ggtgcagcgt ctggtggcca ggtcgatgcg
     7861 gctaacctga ttaaaccgtt gctctccagc ggtaaaatcc gcgtaattgg ttcgacaacc
     7921 tatcaggagt tcagcaacat tttcgagaaa gaccgtgctc tggcgcgtcg cttccagaaa
     7981 attgatatta ctgaaccgtc aatcgaagaa actgttcaaa tcatcaatgg cctgaaaccg
     8041 aagtatgaag cgcaccacga cgtgcgttat accgcaaaag cggtgcgtgc ggcggtagag
     8101 ctggcggtga aatacattaa cgatcgtcat ctgccggata aagcgattga tgttatcgac
     8161 gaagcgggcg ctcgcgcacg tctgatgccg gtaagcaaac gcaagaaaac cgttaatgtg
     8221 gcggatattg aatccgtggt ggcccgtatt gcacgcattc cagagaagag tgtttctcag
     8281 agtgaccgcg ataccctgaa aaacctcggc gatcgcctga aaatgctggt cttcggtcag
     8341 gataaagcca ttgaggcgct gactgaagcc attaagatgg cgcgtgcagg tttaggtcac
     8401 gaacataaac cggtcggttc gttcctgttt gccggcccta ctggggtcgg gaaaacagag
     8461 gtgacggtac agctttcgaa agcgttgggc attgagcttc tgcgctttga tatgtccgag
     8521 tatatggaac gccataccgt cagccgtctg attggtgcgc ctccgggata cgttggtttt
     8581 gatcagggcg gtttgctgac tgatgcggtc atcaagcatc cacatgcggt gctgctgctg
     8641 gacgaaatcg agaaagcgca cccggacgtg ttcaatattc tgttgcaggt gatggataac
     8701 ggtacgctga ccgataacaa cggacgcaaa gcggacttcc gtaacgtggt gctggtgatg
     8761 accactaatg ccggggtacg ggaaactgag cgcaaatcca ttggtcttat ccaccaggat
     8821 aacagcaccg atgcgatgga agagatcaag aagatcttta caccggaatt ccgtaaccgt
     8881 ctcgacaaca ttatctggtt cgatcatctg tcaaccgacg tgatccatca ggtggtggat
     8941 aaattcatcg tcgagttgca ggttcagttg gatcagaaag gtgtttctct ggaagtgagc
     9001 caggaagcgc gtaactggct ggccgagaaa ggttacgacc gggcaatggg cgcacgtccg
     9061 atggcgcgtg tcatccagga caacctgaaa aaaccgctcg ccaacgaact gctgtttggt
     9121 tcgctggtgg acggcggtca ggtcaccgtc gcgctggata aagagaaaaa tgagctgact
     9181 tacggattcc agagtgcaca aaagcacaag gcggaagcag cgcattaatc tgattgtctg
     9241 gtaggttggt gaagtccgta atctcgtcag gggttacgga ctttttattt atggggggag
     9301 gaggttcaga cccttttttt gatgatgatg gtaagttatt gataattagt gctgcgggta
     9361 ggtaaggata aaaaagggtg gcagcaggag attgagatgg ttttgcttta ttaacaacgg
     9421 gctaaacgtg tagtatttga gttcactgcc gtacaggcag cttagaaatt gccgcggatc
     9481 ctgtctgcca ataatgacaa gttcactgcc gtacaggcag ataaaatgcg aaaaaaaagc
     9541 ccgtactttc gtacgagctc ttctttaaat atggcggtga gggggggatt cgaacccccg
     9601 atacgttgcc gtatacacac tttccaggcg tgctccttca gccactcgga cacctcacca
     9661 aattgttttt ttgcctgacc tcatgggggg caacggggcg ctactatagg gagttggaat
     9721 aaaacggtca agaagaattt ttatgataat tattgtttgc tcatactgta aacaagttgt
     9781 gcagtatatc tacatcgaga caagttacgg acttatactt ctaaagtact ccatacatat
     9841 cacaaaataa aaaggccggc taaaccgacc ttttactcat tctttctctt cgcccatcag
     9901 gcggtaaaac aatcagcgac tacggaagac aatgcggcct ttgctcaggt cgtacggggt
     9961 cagttcaaca gtcactttgt cgcccgtcag gatgcggatg tagtttttgc gcattttacc
    10021 ggagatgtgt gcagtaacca cgtgaccgtt ttctaactct acgcggaaca tggtattagg
    10081 caacgtttca agaacggtac cttgcatttc aatattgtct tctttggcca tctaatcctc
    10141 tggggtatca ctaccgtaat ttgaaccggc aagataatgc cgaagttctg taaataagta
    10201 aagatttgcg cgctaaatcg caacaaacag gttcggcaca ttactccgaa aacacacggc
    10261 taagccgcac caaaagcgca acgtataagg gagcggtgag ataaacgatg ggcgttacct
    10321 gacgcgaaaa attccttatc ggcagcgggg taatgagcgt aaccaactct gcgaccgcaa
    10381 ttataacact ctggggagaa atgtgccgaa aacattcatt cttgtggtga aaacaagcat
    10441 cgaggtaccc agaaattatt cgacaatcgt ccgaggcgca tttgattgag ataattaagg
    10501 taatcccggc ggggaatttc gcaggcacca agcgatgctg tgtgatcgtt aaggacctgg
    10561 cagtcgataa gtttaccgcc atgacggata aattcatcac agaagaccag aagcgccgtt
    10621 ttggacgcat tttccatccg gctgaacatg gactcgccac aaaatagtgt tccctgggcc
    10681 acgccgtaca taccgccgac aagctcatct tcacgccaga cttcaatgga gtgggcatga
    10741 ccgagttcgt gaaggcgatg gtaggcttcg accacgccac gcgtgatcca ggttccttct
    10801 tcgcgatcgc tggcacagcc ttcgatgacc tgaccaaaag cgtaattcat cgtgacacga
    10861 tagggtgagc gtttatgaaa tcgcttcata ctacggctga tatgcagtga ttctggccat
    10921 aacaccgcgc ggggatcggg cgaccaccag aggatggggt cgccaggaga aaaccacggg
    10981 aaaataccac gctgataagc cattaatagg cgcgcagggc taagatcgcc ccccagtgcc
    11041 agcaggccgt taggctcacg taatgcgcct tccggggaag ggaaggctat tgaatggcga
    11101 gaaagctgaa ccaggcgcat gaccgcaaaa ctccacgcaa gtcggatcgt tcaataatag
    11161 cttacaaacc ctgcttaaac tggtaataac gcccctgtct ggcaagcagt tctgcgtgag
    11221 taccttgctc aataatttgc ccgttgtcca tcactattat ttgttggaaa cgagagagcc
    11281 cgcgaagtcg atgggtgacc attaacaccg ttttctcgca catcatttct gcaagcaatt
    11341 caaggatctg actttcggtt gtggcatcta agccttctgt aggttcatcc agcaatacca
    11401 gcggcgcatc atgtaacagc gcacgggcga tagccagacg gcgcagttca ccaccggaga
    11461 gctggcgtcc gccttcacct aaccaactgt tgagacctgc atcctcgagc agcttctcca
    11521 gaccaacgcg acgcaagatc tccgccagag cctcatcact actaccaggc gaggcgagta
    11581 acagattatc acgcagcgtg gcgctaaaca gatgcactcg ctggggaaca acgctgatgg
    11641 tctgtcgtag agccgcttca ttcaggctgg ctatgggact atcgttaagc aaaatctcgc
    11701 cctgttgcgg gtcccatgcg cgggtcagca gttgtaacag tgttgacttg ccgcatccgg
    11761 ttcgcccgag aatcgcaata tgttccccgg cgtttacctg aagataaatc cctttaagtg
    11821 cctgttggga ttgctccgga taagtgaact gaacatcccg taatgtcagc gaaacgcgat
    11881 cggcaacacg agtttgggta tcagggaagg tgactttcgg tttttgatcc gttaaatcag
    11941 tgatccgtac ggcagaggca atgacttgcc ccagatgctg aaatgcaccc gttactggtg
    12001 ccagcgcttc aaacgcggct aacgcgcaga agacaaacag ggcaattaac gcgccgggtt
    12061 gagcattgcc gccaacgccg ccagacgcca tccacagcat caggatcacc gctaacgcgc
    12121 caatgagcag cattatcgct tgcgacaatg cggttagttc agattgacgg cgttgcgctt
    12181 ccagccattg aatttctgta ttctctaatt gcgtgcgata acgatcgctg gcaccaaaaa
    12241 tggtcagctc agcttgccct tgcagccagg ccgtcagttg ttggcgatac tgcccgcgaa
    12301 gatgagtcag attttgcccg gtgctttttc ccgcacgata aaacagtggt ggcatcagga
    12361 aaagcgtcag taacataatg ccgcccagcg taaaggcgag ggtgaaatca aggaaactta
    12421 acccgattgt caccaccata atcaccacaa aagcgcctac cagcggcgag ataacgcgca
    12481 ggtaaagatg atcgagcgta tcaacatccg ccaccacgcg gttgagtaat tcgccctgac
    12541 gatagcgcgc cagtcctgca ggggagaggg gcagtaattt gctgaaggtg taaatgcgca
    12601 gatgctgcaa cacgcggaaa gtcgcatcgt gacttaccag gcgttcaaaa tagcgcccgg
    12661 cagtacgggt gattgctgcg ccacgcacgc ccgcagcggg tagcatatag ttgaagctgt
    12721 acagtccggc aacccccgca accgctgagg cggagaggaa ccagcccgaa agtgtcaaca
    12781 gaccgatact ggcgagcagc gtgacaattg ccagcacgat accaagactt aacatccatt
    12841 tatgacgttt atacagtgcc agatagggta gcaaagcgcg catttaaatc tcctcctgac
    12901 gatgggccag taatgtggcg aatgggccac cagccacact taattccgcg taacgtcctt
    12961 gctcaataat ctgaccatcc tgcattaccc aaatgacatc ccagtcagca agatcttcta
    13021 actggtgggt gaccattaac gttgtctggc gcagagaggc ggcattcagc gcctccatta
    13081 cgcgctgttc actgtgagca tcaaggctgg cagcgggttc atccaacagt aatagcgaac
    13141 agggatttag taacgcacgg gccaccgcca cgcgctgcgc ctgccccacg gaaaggcggg
    13201 cagcctggtc gccaacaggc gtatcaacgc cttgtggcag gagcggtaga aactcactga
    13261 cccaggcgtt atccagcgct gtttgtaact cttgttcgct ggcatcaggt cgcgccagta
    13321 gtacgttatc ccgtaatgtt gctgccggta attgtgggtt ttgcccaacc caggagagat
    13381 gtttacgcca tgattccggt gataaatcgc gtaattctat cccgttgatt cgtagcgatc
    13441 cctgatatga gagaaaacca gaaagcgcgt tcaacagtga acttttacct gaaccgctgc
    13501 gaccaaccaa cactgctcgt tggcctgctg gcaaagtaaa attcagcggt ccggccagcg
    13561 ttttaccttc cggcgacgtg ataaacagat cctcggcttc aatggtcacc ggatcggtcg
    13621 atgctaattc cgcttcaccg cgctgcggat gggctagcgg ggtttccata aacgttttca
    13681 aactgtcagc tgcaccaaca gcctgggctt tagcatgata aaacgtaccg agatcgcgta
    13741 atggctggaa aaactctggc gcaaggatca gggccagaaa acccgcagcc agcgtcacgc
    13801 cagtatcgta gtgaccaaaa tccagctcgc cgaggtagga aaaaccaaag tagaccgcca
    13861 ccagagcaat cgacagcgag gtaaaaaatt cgagaatgcc ggaggataaa aacgccagcc
    13921 gtagcacttc cattgtccgt tggcggaaat cttccgaagc agaacgaata ctttcaattt
    13981 cagcttcacc acgaccaaaa atacgcaatg tttccatgcc gcgcaggcga tcgaggaaat
    14041 gcccacttaa gcgagcaaga gcgagaaagt tacgtcggtt agcatcggca gcccccattc
    14101 caaccagcgc cataaataac ggaattagcg gtgcagtgcc cagcagaatg agcgccgcgg
    14161 cccagttaga ggggaagata gccaccacaa tcagcagcgg caccgacact gccagcgcca
    14221 tttgcggcag gtagcgtgca tagtaatcat gcatatcgtc aatttgctcg agtaccagcg
    14281 tcgcccagct ccccgcaggt ttaccctgaa tccacgctgg ccctgcttgt tgcagacggt
    14341 cgagaacctg acggcggatg gcaaagcgga tatgctgccc ggcgtgataa cccacccgtt
    14401 cgcgtaacca gaccacccat gcgcgcagta caaaggtcag aaacagtaac gtaaagggaa
    14461 gcagcagggc ttcacgggga atattctcca taatcatatg ttgcagaatt cgcgccatga
    14521 accaggcctg ggcaatgatc aatatgccgc tcacaaagcc cagcagacga gaaatattca
    14581 gccaacgttg ggagatgacg ctttgctgtt ttaaccagcg ggttaattct ttttgacggg
    14641 atttattcat tgcacgctta gcaggtgagt tatcagaatt atttgcagag caatgttaca
    14701 acggggaaaa aataaaggcg acccatagtc gcatggtgtc gccttcttta cttttgttac
    14761 tgatttgtaa aattattttg cgtcagctaa accgtcgagg tagcgttccg catcaagagc
    14821 tgccatgcag cctgtaccag cagatgtaat ggcctggcga taaatgtgat ccatcacgtc
    14881 gcctgcggca aagacgccag ggatgctggt ctgggtggca ttaccatgaa tacccgactg
    14941 tactttgatg tagccgtttt ccagttccag ctgcccttcg aaaatggcag tattcgggct
    15001 gtgaccgata gcaacaaaca gaccggcaac gtcgagtgac tcgatattat cggtgttttg
    15061 cgtatcgcgc agacgaacgc cagtgacgcc catctgatcg ccggtcactt cttccagcgt
    15121 acggttggtg tgcagaataa tgttgccgtt ctccacttta tccatcagac gcttaatgag
    15181 gattttttcc gcacggaaac catcacggcg gtgaatcaga tgaacttccg aagcgatgtt
    15241 agacagatac agcgcctctt caaccgcggt attgccgccg ccgatgaccg caactttctg
    15301 gttgcgatag aagaaaccgt cgcaggttgc acaagcagaa actccacggc ctttaaaggc
    15361 ttcttcagag ggcaggccga gatagcgtgc agaagctccg gtggcaataa tcagcgcgtc
    15421 gcaagtgtat tcgccgctat cgccagtcag acggaacgga cgattctgca aatcaacttt
    15481 gttgatgtga tcaaagatga tttcagtttc aaatttggtg gcatgttcgt gcatgcgctc
    15541 cattaataac ggaccggtca gatcgtttgg atcgccaggc cagttttcca cttccgtggt
    15601 ggtggtcagt tggccgcctt tttccatgcc ggtaatcagc acaggttgca ggttggcgcg
    15661 cgccgcgtag acagcagcgg tgtatcccgc cgggcctgaa cccaggataa gcagtttact
    15721 gtgtttggtc gtgcccatga gatccccata gttgttggca gacaatggca ggattgtagg
    15781 gaatttacag acgtaaaaaa agagtatgac gattttgtta acaatttgtg caatcggcag
    15841 catcgataag caggtcaaat tctcacgtca ttatcacctc tgctacttaa atttcccgct
    15901 ttataagccg attaaatgat gaataaacgc ccctgttaat gaatatctgg catgttgtac
    15961 taaaaatcga tgttttgctt tgacaatccc ctggtgtttt gcgaaaacat tcgaggaaga
    16021 aaaaaaacag tattcttata tgcgcataac catgcatgta aataccatgt ttaccgtgct
    16081 agtgaaatct acgtatggcg tggacagacg ccattcgtga tgtcgatagc tgccacaagg
    16141 caacggtctt ctcaccgtag acccaggcat tgcgcgccgt gaatcttcat gatttcggtc
    16201 tatcgtgacg ggtagcgact ctgaacagtg atgtttcagg gtcagacagg agtagggaag
    16261 gaatacagag agacaataat aatggtagat agcaagaagc gccctggcaa agatctcgac
    16321 cgtatcgatc gtaacattct taatgagttg caaaaggatg ggcgtatttc taacgtcgag
    16381 ctttctaaac gtgtgggact ttccccaacg ccgtgccttg agcgtgtgcg tcggctggaa
    16441 agacaagggt ttattcaggg ctatacggcg ctgctgaacc cccattatct ggatgcatca
    16501 cttctggtat tcgttgagat tactctgaat cgtggcgcac cggatgtgtt tgaacaattc
    16561 aataccgctg tacaaaaact tgaagaaatt caggagtgtc atttagtatc cggtgatttc
    16621 gactacctgt tgaaaacacg cgtgccggat atgtctgctt accgtaagtt actgggggaa
    16681 accctgctgc gtctgcctgg cgtcaatgac acacggacat acgttgttat ggaagaagtc
    16741 aagcagagta atcgtctggt tattaagacg cgctaacacg gaacaggtgc aaaatcggcg
    16801 tattttgatt acactcctgt taatccatac agcaacagta ctggggtaac ctggtactgt
    16861 tgtccgtttt agcatcgggc aggaaaagcc tgtaacctgg agagcctttc ttgagccagg
    16921 aatacactga agacaaagaa gtcacattga caaagttaag tagcggccgc cgccttctgg
    16981 aggcgttgct gatccttatt gtcctgtttg ccgtctggtt gatggctgcc ttactaagct
    17041 ttaacccttc ggaccccagc tggtcgcaaa cggcctggca tgaacctatc cataatttag
    17101 gtgggatgcc cggtgcgtgg ctggcagata cgctgttctt tatttttggc gtgatggctt
    17161 acaccattcc cgtcattatt gtcggcggtt gttggtttgc ctggcgtcat cagtccagcg
    17221 acgaatacat tgattatttt gccgtttcgc tacgcatcat tggcgttttg gcgctcatcc
    17281 ttacctcctg tggtctggcg gcaatcaacg ctgacgatat ctggtatttt gcctccggtg
    17341 gcgtcattgg cagcttacta agcactacgc tacaaccact gctacacagt agcgggggaa
    17401 ctattgcgct gctctgcgtt tgggcagcgg gcctgacgct gttcaccggt tggtcatggg
    17461 tgaccattgc tgaaaaactc ggcggctgga ttttaaatat tcttaccttc gccagtaatc
    17521 gtacccgtcg cgatgatacc tgggtcgatg aagatgagta tgaagacgac gaagagtatg
    17581 aagatgaaaa tcacggcaaa cagcatgaat cacgccgtgc ccgtattctt cgcggcgcgc
    17641 tagcgcgtcg taaacggttg gcggaaaaat tcattaatcc gatggggcgg caaacagacg
    17701 ctgcgttgtt ctccggtaag cggatggatg atgacgaaga gattacctac actgtacgcg
    17761 gtgtggctgc cgaccctgac gacgtcctct tttcgggcaa tcgtgcaacg cagccagaat
    17821 atgacgagta cgatccatta ttaaacggtg cgccaattac cgaacctgtt gctgtagcag
    17881 ctgctgctac cacggcgaca caaagctggg ctgcaccggt tgaacctgtg actcagacgc
    17941 cgccagttgc ctctgttgat gttccacctg cgcaatctac agtagcctgg cagcctgtac
    18001 cggggccgca aacgggagaa ccggttattg ctcctgcacc ggaaggttac ccacagcagc
    18061 cacaatatgc gcagcctgca gtgcaatata atgagccgct gcaacaacca gtacagccac
    18121 agcagccgta ttatgcacct gcagctgaac aacctgcgca acaaccttac tacgctcctg
    18181 cggcggaaca acctgttcag caaccgtatt acgcaactgc ggcggaacag cctgcgcaac
    18241 agccgtatta cgcccctgcg ccagaacaag cggtggcagg taacgcctgg caagccgaag
    18301 agcagcaatc cacttttgct ccacagtcta cataccagac tgagcaaact tatcagcagc
    18361 cagccgctca ggaaccgttg taccaacagc cgcaacctgt tgaacagcag cctgttgtgg
    18421 agcctgaacc cgttgtagaa gagacaaaac ccacgcgtcc gccgctttac tactttgaag
    18481 aagtggaaga gaagcgagcc cgtgaacgcg aacaacttgc ggcctggtat caaccgattc
    18541 cagaacctgt taaagaacca gaaccgatca aatcttcgct aaaagcacct tctgttgcag
    18601 cagtacctcc agtggaagcc gctgccgccg tttctccgct ggcatctggc gtgaaaaaag
    18661 cgacactggc gacgggggct gccgcaaccg ttgccgcgcc agtcttcagt ctggcaaata
    18721 gcggtggacc gcgtccgcag gtcaaagagg ggattggccc gcagttgcca cgaccgaaac
    18781 gcatccgcgt gccaactcgt cgtgaactgg cgtcttacgg tattaagctg ccctcacagc
    18841 gtgcggcaga agaaaaagcc cgtgaagccc agcgtaatca gtacgattct ggcgatcagt
    18901 acaacgatga tgaaatcgat gcgatgcagc aggatgaact ggcacgtcag ttcgcccaga
    18961 cgcagcagca acgctatggc gaacagtatc aacatgatgt gcccgtgaac acagaagatg
    19021 cagatgctgc ggcagaggca gaactggctc gtcagtttgc tcaaactcaa caacaacgtt
    19081 attccggcga acaaccggct ggggcgaatc cgttctcgct ggatgatttt gaattttcgc
    19141 caatgaaagc gttgctggat gatggtccac acgaaccgct gtttacgcca attgttgaac
    19201 ctgtacagca gccgcaacaa ccggtagcac cgcagcagca atatcagcag ccgcaacaac
    19261 cggttgcacc gcagccgcag tatcagcagc cgcaacaacc ggttgcaccg cagcagcaat
    19321 atcagcagcc acaacaaccg gttgcacagc agccgcagta tcagcagcca caacagccgg
    19381 ttacacagca gccgcagtat cagcagccgc agcagccggt tgtaccacag ccgcaatatc
    19441 agcagccgca acagccagtt gcgccacagc cgcaggatac cctgcttcat ccgctgttga
    19501 tgcgtaatgg cgacagccgt ccgttgcata aaccgacgac gccactgcct tctctggatt
    19561 tgctgacacc gccgccgagc gaagtggagc cggtagatac ctttgcgctt gaacaaatgg
    19621 cgcgcctggt agaagcgcgt ctggctgatt tccgtattaa agccgatgtt gtcaattact
    19681 ctccggggcc agttatcact cgctttgaat tgaacctggc accgggcgta aaagcggcgc
    19741 gcatttctaa cttgtcacgt gaccttgccc gttcactttc gacggtggcg gtgcgtgtcg
    19801 ttgaagttat tcctggcaaa ccctatgtag gtctggagtt accgaataaa aaacgacaaa
    19861 ccgtttatct gcgcgaggtt ttggataacg ccaaattccg cgataatccg tcgccattaa
    19921 ccgtggtgct gggtaaagat atcgccggtg agccggtggt tgccgatctg gcgaaaatgc
    19981 cgcacttgtt ggttgcgggg actaccggtt ccggtaagtc tgtcggggtg aacgcgatga
    20041 tcctgagcat gctttataaa gcacagccag aagatgtgcg tttcatcatg atcgacccga
    20101 aaatgctgga gctttcggtt tatgaaggca ttccgcacct gttaacggaa gttgttactg
    20161 atatgaaaga tgccgccaac gcgctgcgct ggtgtgttaa cgagatggag cgtcgttata
    20221 aactgatgtc tgcgctgggt gtgcgtaatc tggcgggtta taacgaaaaa attgctgaag
    20281 ccgatcgcat gatgcgtccg attccagacc cgtactggaa gccgggtgat agtatggatg
    20341 cgcagcatcc ggtgctgaaa aaagaaccgt acatagtggt gttggtggac gaatttgccg
    20401 acctgatgat gacggtaggt aaaaaagtgg aagagctgat agcacgtctg gcgcaaaaag
    20461 ctcgtgccgc gggtatccac ctcgtactgg caacgcagcg tccatcggtt gatgttatta
    20521 ctggtctgat taaagcgaat attccgaccc gtatcgcctt taccgtatcc agtaagattg
    20581 actcacgtac cattcttgat caggctggtg cggaatcact gctgggtatg ggggatatgc
    20641 tctactctgg gccgaactcc actttgccgg tacgtgtcca tggtgctttt gttcgcgatc
    20701 aagaagttca tgccgtggtg caggactgga aagcgcgtgg tcgcccacag tatgttgatg
    20761 gcatcacctc cgacagcgaa agcgaaggtg gcgcgggcgg tttcgatggc gctgaagagc
    20821 tggatccgtt gttcgatcag gcggtgcagt ttgtcactga aaaacgcaaa gcatcaattt
    20881 ctggcgtaca gcgtcagttc cgcattggtt ataaccgtgc agcgcgtatt atcgaacaga
    20941 tggaagcgca ggggattgtc agcgaacagg ggcacaacgg taatcgtgaa gtgctggccc
    21001 caccgccgtt tgactaatta atgcatcgta ggccggataa ggcgcggtag cgtcgcatcc
    21061 ggcaatctgg taactgaaaa ttcagtattt tcttctttcc tcaagctgat tattagcctg
    21121 gaatagagag tagagggagc tcccggtcgg gagtgacgta atttgaggaa taatgatgaa
    21181 aaaaattgcc atcacctgtg cattactctc aagcttagta gcaagcagcg tttgggctga
    21241 tgccgcaagc gatctgaaaa gccgcctgga taaagtcagc agcttccacg ccagcttcac
    21301 acaaaaagtg actgacggta gcggcgcggc ggtgcaggaa ggtcagggcg atctgtgggt
    21361 gaaacgtcca aacttattca actggcatat gactcaacct gatgaaagca ttctggtttc
    21421 tgacggtaaa acactgtggt tctataaccc gttcgttgag caagctacgg caacctggct
    21481 gaaagatgcc accggtaata cgccgtttat gctgattgcc cgcaaccagt ccagcgactg
    21541 gcagcagtac aatatcaaac agaatggcga tgactttgtt ctgacgccga aagccagcaa
    21601 tggcaatctg aagcagttca ccattaacgt gggacgtgat ggcacaatcc atcagtttag
    21661 cgcggtggag caggacgatc agcgcagcag ttatcagctg aaatcccagc aaaatggggc
    21721 tgtggatgca gcgaaattta ccttcacccc gccgcaaggc gtcacggtag atgatcaacg
    21781 taagtagagg cacctgagtg agcaatctgt cgctcgattt ttcggataat acttttcaac
    21841 ccctggccgc gcgtatgcgg ccagaaaatt tagcacagta tatcggccag caacatttgc
    21901 tggctgtggg gaagccgttg ccgcgcgcta tcgaagccgg gcatctgcat tctatgatcc
    21961 tctgggggcc gccgggtacc ggcaaaacaa ccctcgctga agtgattgcc cgctatgcga
    22021 acgctgatgt ggaacgtatt tctgccgtca cctctggcgt gaaagagatt cgcgaggcga
    22081 tcgaacgcgc tcggcaaaac cgcaatgcag gtcgccgcac tattcttttt gttgacgaag
    22141 ttcaccgttt caacaaaagc cagcaggatg catttctgcc acatattgaa gacggcacca
    22201 tcacttttat tggcgcaacc actgaaaacc cgtcgtttga gcttaattcg gcactgcttt
    22261 cccgtgcccg tgtctatctg ttgaaatccc tgagtacaga ggatattgag caagtactaa
    22321 ctcaggcgat ggaagacaaa acccgtggct atggtggtca ggatattgtt ctgccagatg
    22381 aaacacgacg cgccattgct gaactggtga atggcgacgc gcgccgggcg ttaaatacgc
    22441 tggaaatgat ggcggatatg gccgaagtcg atgatagcgg taagcgggtc ctgaagcctg
    22501 aattactgac cgaaatcgcc ggtgaacgta gcgcccgctt tgataacaaa ggcgatcgct
    22561 tttacgatct gatttccgca ttgcataagt cggtacgtgg tagcgcaccc gatgcggcgc
    22621 tctactggta tgcgcgaatt attaccgccg gtggcgatcc gttatatgtt gcgcgtcgtt
    22681 gcctggcaat tgcttccgaa gatgtcggca atgccgaccc acgggcgatg caggtggcaa
    22741 ttgcggcctg ggattgcttt actcgcgttg gcccggcgga aggtgaacgc gccattgctc
    22801 aggcgattgt ttacctggcc tgcgcgccaa aaagcaacgc tgtctacact gcgtttaaag
    22861 ccgcgctggc cgatgctcgc gaacgtccgg attatgacgt gccggttcat ttgcgtaatg
    22921 cgccgacgaa attaatgaag gaaatgggct acgggcagga atatcgttac gctcatgatg
    22981 aagcaaacgc ttatgctgcc ggtgaggttt acttcccgcc ggaaatagca caaacacgct
    23041 attatttccc gacaaacagg ggccttgaag gcaagattgg cgaaaagctc gcctggctgg
    23101 ctgaacagga tcaaaatagc cccataaaac gctaccgtta atgttatcgt tgcggtaatg
    23161 ttgttaatgt atccctgtgg tcgcaggctg tggccacatc tcccatttaa ttcgataagc
    23221 acaggataag catgctcgat cccaatctgc tgcgtaatga gccagacgca gtcgctgaaa
    23281 aactggcacg ccggggcttt aagctggatg tagataagct gggcgctctt gaggagcgtc
    23341 gtaaagtatt gcaggtcaaa acggaaaacc tgcaagcgga gcgtaactcc cgatcgaaat
    23401 ccattggcca ggcgaaagcg cgcggggaag atatcgagcc tttacgtctg gaagtgaaca
    23461 aactgggcga agagctggat gcagcaaaag ccgagctgga tgctttacag gctgaaattc
    23521 gcgatatcgc gctaaccatc cctaacctgc ctgcagatga agtgccggta ggtaaagacg
    23581 aaaatgacaa cgttgaagtc agtcgctggg gcaccccgcg tgagtttgac tttgaagttc
    23641 gtgaccatgt gacgctgggt gaaatgcact ctggcctcga ctttgcagcc gcagttaagt
    23701 tgactggttc ccgctttgtg gtaatgaaag ggcagattgc tcgcatgcac cgcgcactgt
    23761 cgcagtttat gctggatctg cataccgaac agcatggcta cagtgagaac tatgttccgt
    23821 acctggttaa ccaggacacg ctgtacggta cgggtcagct gccgaaattt gctggcgatc
    23881 tgttccatac tcgtccgctg gaagaagaag cagacaccag taactatgcg ctgatcccaa
    23941 cggcagaagt tccgctgacc aacctggtac gcggtgaaat catcgatgaa gatgatctgc
    24001 caattaagat gaccgcccac accccatgtt tccgttctga agctggttca tatggtcgtg
    24061 acactcgtgg tctgatccgt atgcaccagt tcgacaaagt tgaaatggtg cagatcgtgc
    24121 gcccggaaga ctcaatggcg gcgctggaag agatgaccgg tcatgcggaa aaagtcctgc
    24181 agctgctggg cctgccgtac cgtaaaatca tcctttgtac cggcgacatg ggctttggcg
    24241 cttgcaaaac ttatgacctg gaagtatgga tcccggcaca gaacacctac cgcgagatct
    24301 cttcctgctc caacgtttgg gatttccagg cacgtcgtat gcaggcacgt tgccgcagca
    24361 agtcggacaa gaaaacccgt ctggttcata ccctgaacgg ttctggtctt gctgttggtc
    24421 gtacgcttgt tgcagtaatg gaaaactatc agcaggctga tggtcgtatt gaagtaccag
    24481 aagttctgcg tccgtatatg aatggactgg aatatattgg ctaataccca atttttctga
    24541 atctaaaaag cgcctgcggg cgcttttttt gtctcccttt gataccgaac aataattact
    24601 cctcacttac aagtaatact actttcgagt gaaaatccac ctatctcttt gattttcaaa
    24661 ttattcgatg tatacaagcc tatatagcga tctgctatag aaataattac acaatacggt
    24721 ttgttactgg aatcaatcgt gagcaagctt gagtgagcca ttatgaaaac gaaaatccct
    24781 gatgcggtat tggctgctga ggtgagtcgc cgtggtttgg taaaaacgac agcgatcggc
    24841 ggcctggcaa tggccagcag cgcattaaca ttacctttta gtcggattgc gcacgctgtc
    24901 gatagcgcca ttccaacaaa atcagacgaa aaggttatct ggagcgcctg tacagttaac
    24961 tgtggtagtc gctgcccgct acgtatgcac gtcgtggacg gtgaaatcaa atatgttgaa
    25021 acggataata ctggcgatga caactatgac ggcctgcacc aggtgcgcgc ctgtctgcgt
    25081 ggtcgttcca tgcgtcgccg cgtttacaat cccgaccgcc tgaaatatcc gatgaaacga
    25141 gtcggggcgc gcggtgaagg caaattcgag cgcattagct gggaagaagc ctacgacatc
    25201 atcgcgacca atatgcagcg cctgatcaaa gagtacggca acgaatccat ctatctgaac
    25261 tatggcaccg gtacgctggg tggcaccatg acccgctcct ggccgccggg aaataccctg
    25321 gtcgcgcgcc tgatgaactg ctgcggcggt tatctgaacc attacggtga ctactcttcc
    25381 gcgcaaattg cggaaggttt gaactatacc tacggcggct gggcagatgg taacagcccg
    25441 tcggatatcg aaaacagtaa actggtagtg ctgtttggta ataaccctgg cgaaacgcga
    25501 atgagtggcg gtggggtgac ttactatctt gaacaggcgc gccagaaatc taatgctcgc
    25561 atgatcatca tcgatccgcg ctataccgac accggtgccg ggcgcgaaga tgagtggatc
    25621 cctattcgtc cgggaacaga tgccgcactg gttaacggtc tggcgtatgt catgatcacc
    25681 gaaaacctgg tggatcaggc attcctcgat aaatattgcg ttggctacga cgagaaaaca
    25741 ctgccagcca gtgcgccgaa aaatggtcac tataaagctt atattctggg tgaagggcca
    25801 gatggcgtgg ctaaaacgcc ggaatgggcc tcgcaaatca ctggtgttcc ggcagacaaa
    25861 atcatcaaat tggctcgtga aatcggtagt accaaaccgg cgtttatcag ccagggatgg
    25921 ggcccgcagc gtcacgctaa cggtgaaatc gcaacccgtg ctatctcgat gctggcgatt
    25981 ctgaccggta acgttggtat taacggaggc aacagcggcg cgcgtgaagg ttcatacagc
    26041 ttaccgtttg tccgtatgcc gaccctcgaa aacccgatcc agaccagcat ttcgatgttt
    26101 atgtggaccg atgccattga acgtggcccg gaaatgacag cgctgcgtga tggtgtgcgc
    26161 gggaaagata agctggatgt gccgatcaaa atgatctgga actatgccgg taactgcctg
    26221 attaaccagc attctgaaat caaccgtacc catgaaatcc ttcaggatga taagaagtgc
    26281 gagctgattg tggttatcga ctgccacatg acctcatcgg cgaaatatgc tgacatcctg
    26341 ctgcctgact gcaccgcttc agaacagatg gactttgcac tggatgcatc ctgcgggaat
    26401 atgtcttacg tgattttcaa cgatcaggtg attaaaccgc gctttgaatg taagaccatc
    26461 tatgaaatga ccagcgaact ggcaaaacgt cttggcgttg agcaacagtt tactgagggc
    26521 cgtacccagg aagagtggat gcggcatctg tatgcccagt cgcgggaagc gattcctgaa
    26581 ctgccaacgt ttgaagagtt ccgtaagcag gggattttta aaaagcgcga cccacaaggg
    26641 catcacgttg cttataaagc tttccgtgaa gatccgcagg caaatccact gaccacgcca
    26701 tcggggaaaa ttgagattta ttcgcaggcg ctggctgaca ttgccgctac ctgggaattg
    26761 ccagaaggtg atgtgatcga tccactgccg atctatacgc cgggttttga aagttatcag
    26821 gatccgctga acaaacagta tccgctgcag cttacgggtt tccactataa atctcgcgtt
    26881 cactcaactt acggcaacgt tgatgtgctg aaagcagctt gccgtcagga aatgtggatc
    26941 aacccgcttg atgcgcaaaa acgcggtatc aacaacggcg ataaagtcag gatctttaac
    27001 gatcgtggtg aggttcatat tgaggcgaaa gtgacgccac gaatgatgcc gggtgtggtc
    27061 gcactgggtg aaggtgcctg gtatgacccg gatacaaaac gtgtcgataa gggcggttgt
    27121 attaacgtac tgaccactca acgtccgtct cctctcgcta aggggaatcc gtcacataca
    27181 aaccttgttc aggttgaaaa ggtgtaagga gtaaccgatg acaacccagt atggattttt
    27241 tattgattcc agccgttgca ccggttgcaa aacctgcgag ctggcttgta aagactacaa
    27301 agatttgacg ccagaagtca gcttccgccg catttatgaa tatgctggtg gcgactggca
    27361 ggaagataac ggtgtctggc accagaacgt gtttgcctac tatctgtcga tttcatgtaa
    27421 ccactgcgaa gatccggctt gtactaaagt ctgcccgagc ggtgcgatgc ataaacgtga
    27481 agatggtttt gttgtggtcg atgaagatgt atgcattggc tgccgttact gtcatatggc
    27541 ttgcccgtat ggcgcaccgc aatataacga aacgaaaggc catatgacca aatgcgatgg
    27601 ttgttatgac cgtgttgcgg agggaaaaaa accgatctgc gtagagtcct gcccgctacg
    27661 ggcgctggat ttcggcccta tcgacgagct gcgtaaaaaa catggcgatc tggcggccgt
    27721 tgcgccgttg ccgcgagctc actttaccaa accgaatatt gtgatcaaac ccaatgccaa
    27781 cagccgccca accggggata ccactggcta tctggcaaac ccgaaggagg tgtgagatgg
    27841 gaagtggatg gcatgaatgg ccgctgatga tcttcacggt cttcgggcaa tgtgtcgcgg
    27901 gtggttttat cgttctggct ttggcgctgc tcaaaggcga cctgcgagca gaagcccagc
    27961 agcgtgttat cgcctgcatg ttgggtttat gggtgctgat gggcattggc tttatctcct
    28021 ctatgcttca tcttggttca ccgatgcgcg cttttaactc gctcaatcgg gtaggggctt
    28081 cagcactcag taacgaaatc gccagcggtt cgatcttttt tgctgtaggc ggcatcggct
    28141 ggttgctggc aatgctgaaa aagctttcac cggcattgcg tacgctttgg ctggtggtga
    28201 cgatggttct tggcgtagtc tttgtctgga tgatggtgcg tgtgtataac agcatcgata
    28261 cagtacccac ctggtacagc atctggacac cgatgggctt cttcctgacg atgtttatgg
    28321 gcggcccgtt actgggttac ctgttgctga gtctggctgg cgtcgatggc tgggcgatgc
    28381 gtctgctgcc agccatttct gttctggcac tggtagtgag tggcgtgatg tcagtgatgc
    28441 agggcgcaga gctggcgact attcatagct ctgtgcagca ggccgcagcg ctggtgccgg
    28501 actatggtgc gctgatgtcc tggcggatcg tgcttttggc cgttgccctg tgcgtgtgga
    28561 ttgcaccaca gctaaaaggt tatcagcctg cggttccgct actttctgta tcattcattc
    28621 tgcttctggc aggggaatta atcggtcgtg gcgtattcta tggtttgcat atgaccgtgg
    28681 ggatggctgt cgcaaattaa tcagtaacaa ccggggtttc ggccccggtt cgcttttatt
    28741 tctgcttcgt taacgtgtca taactggtca tcaagttacg ataatcagga atgtggttag
    28801 agaacaacgt cgccagtcct tcaatatcat tacgccagtc gcgatgcagc tcgcacgcca
    28861 cgccgaacca tgtcatcaat tgcgcaccag cttgcgacat gcgatcccat gccgaatgcc
    28921 gggtaatttc attaaaggtc cctgacgcgt cagtcacgac aaagacgtca aacccttctt
    28981 caatggctga aagcgccggg aatgccacgc aaacttcggt taccacaccg gcaataatta
    29041 actgtttttt acctgtcgct ttgacagctt ttacaaaatc ttcgttatcc caggcgttaa
    29101 tatttcccgg gcgagcaatg taaggtgcat cggggaattg tgctttcagt tctggaacta
    29161 atgggccgtt aggaccggtt tcaaaactgg tggtaagaat agttggtaag ttgaaatact
    29221 tagccaggtc acccagcgcc agcacgttat ttttaaactt atcgggttcg atatcccgta
    29281 caagggaaag taaaccagcc tggtgatcaa caagcaaaac ggcagcatca tttttatcaa
    29341 gacgaacata cggtttggtc atcctcttct cctttcgaga gtcagtggta cgtctaaatc
    29401 ggtcagacgc gatgcattgc tctgaaagca tagacgggaa atatgagttt gctgtgacca
    29461 tgaaattttt cgactgaacg aagcgtatga aatttgtgtt agatcaataa aaacaatcag
    29521 atgagattaa tcgtctcttc agcattagcg ttatgaatga gtaatataaa tcgataatac
    29581 cgccgctgcg atatgcgcgt attttcaccc attgacaatg tttttggcgg tggcatgatg
    29641 cgcatgaaat ttgaacttcc tcacggtttt aattcatgtc cacgtatacc cggcctgtca
    29701 tgcttttgct gtctggcctg cttttgttga ctctggcgat cgcggtgtta aatacactcg
    29761 tgccgctttg gctcgcccag gaacacatgt ctacatggca ggtaggcgtt gtcagctcat
    29821 cctattttac cggcaacctt gtcggtacat tgctgacagg gtatgtcatt aaacgcattg
    29881 gctttaaccg cagctattat ctggcctcct tcatttttgc cgctggctgt gccggccttg
    29941 gcctgatgat tggattctgg agttggttgg cgtggcgttt tgtcgctggc atcggctgtg
    30001 ccatgatttg ggtggttgtt gagagtgcgc tgatgtgcag tgggacgtcg cgtaaccgcg
    30061 ggcggttgct tgctgcgtat atgatggttt attacgtcgg aacgttttta ggccagttac
    30121 tggtcagcaa agtctcaacc gagttgatga gtgtattgcc gtgggttaca ggtttgacgt
    30181 tggcaggaat cttaccgctg ttgtttacgc gtgtcctgaa tcagcagact gaaaaccatg
    30241 attcgacgtc aattacgtca atgctaaaac tccgtcaggc gcggcttggc gtgaatggct
    30301 gcattatctc aggaatcgtg ctgggatctc tatatggcct gatgccgctg tacctcaatc
    30361 acaaaggggt gagcaatgcc agcattggtt tctggatggc ggtactggtc agtgcgggta
    30421 tcctcggaca atggccgatt ggacgtctgg cggataagtt tggtcgactg ctggtgttgc
    30481 gtgttcaggt ctttgtcgtc attctcggca gtattgcgat gcttagccag gcggcgatgg
    30541 ccccagcgtt attcatcctc ggtgccgctg gctttacgct atatccggtg gcgatggcct
    30601 gggcttgtga gaaagttgaa catcatcaac tggtggcgat gaaccaggcc ttactgttga
    30661 gctataccgt gggaagcctg cttggcccgt catttaccgc tatgctaatg cagaatttct
    30721 ccgataattt attgtttatc atgatcgcca gcgtatcgtt tatctatttg ctgatgctgc
    30781 ttcgcaacgc cggccatacg ccgaaacccg tggctcacgt gtaaatgaat tcaagcagag
    30841 tgtgaactta ctgtttcaca ctctgctttt ctgtgcacaa aaaagcggca actcaataaa
    30901 gttgccgctt tacggggaaa ttagaacatg accttatgac cgtactgctc aagaatgcct
    30961 ttcacgcgtt ccatggtctc tttcttcggt ggtttaacac catcgagttt gtattcttca
    31021 cccattgcca cccatttgtg tttgcccagt tcgtggtagg ggaggagctc gattttctca
    31081 acgttgccca tatcacgggt aaattcacca aggcgatgcg ctgaatcgtc atcgtcagac
    31141 cagcctggga caacaacata gcggatccac accttcacat ttttgttcgc cagatattta
    31201 gcgaactcca gcgtgcggtg gttggaaact ccaaccagat tttggtggat ctcgtcgttc
    31261 atctgtttga gatcgagcat taccaggtcg gttacctcca gcagttcatc aatcaccgga
    31321 tcgtaacgac gaacaaaacc gttggtgtcc agacaggtat gaatgccttc ttttttgcag
    31381 gcgcggaacc agtcacgaac aaactcagct tgtaggattg cctcaccgcc ggatgcggta
    31441 acgccgccgc cggaagcgtt cataaagtgg cgataggtca ccacttcctt catcaaatct
    31501 tcaacggtaa cttctttacc gccatgcgta tcccaggtgt cgcggttatg acaatacagg
    31561 cagcgcatca ggcagccctg gaaaaaggtg ataaagcgaa tacccgggcc gtctacggtt
    31621 ccacaggatt caaaggagtg aatgcgacca ataactgaca ttgcggtgtt tctccagatg
    31681 tggcccatct gaggccgtgt tggtgcgcag ctcgaaggct acgtcgagtc tgttttggca
    31741 gtcaccttaa agtatagata gctgacaaaa aaggctctcg tgctaaaaaa ggccccactt
    31801 tcgtggagcc tttattgtac gctttttact gtacgatttc agtcaaatct aattacatag
    31861 attgagtgaa ggtacgagta ataacgtcct gctgctgttc tttagtcagc gagttgaaac
    31921 gtactgcgta gccagataca cggatggtca gctgcggata tttttccggg ttttccatcg
    31981 cgtcgagcag catttcacgg ttcatcacgt taacgttcag gtgctgacca ccttcgatgg
    32041 atgcttcgtg gtggaagtaa ccatccatca gaccagccag gttggtctta cgaacttcgt
    32101 cgtctttacc cagtgcgttc ggaacgatag agaaggtgta ggagatacca tctttagcgt
    32161 aagcaaacgg cagtttagca acggaagtca gagacgctac agcacctttc tggtcacgac
    32221 cgtgcatcgg gttagcaccc ggtccgaacg gcgcgccagc acgacgaccg tctggggtgt
    32281 taccagtttt cttaccatac acaacgttag aagtgatggt cagaacagac tgagtcggga
    32341 tagcgtcacg gtaggtgtgc agtttctgaa ttttcttcat gaaacgttct accaggtcaa
    32401 cagccaggtc atctacacgc ggatcgttgt taccaaactg cgggtattcg ccttcgattt
    32461 cgaagtcgat agccagaccg tcttcatcac gaatcggttt aactttcgca tatttgattg
    32521 cagacaggga gtcagcagca acggacagac cagcgatacc acacgccatg gtgcggataa
    32581 cgtcacggtc gtgcagcgcc atcagagagg cttcgtagct gtacttgtcg tgcatgtagt
    32641 ggatgatgtt cagtgcagtg atgtactgtt tagccagcca gtccatgaag tgatccatgc
    32701 gctccatcac ttcatcatag ttcaggacat cgcctttgat cggctcagat ttcggaccaa
    32761 cctgcatttt cagtttttcg tcaacgccac cgttgattgc gtacagcatg gttttcgcca
    32821 ggtttgcacg cgcaccgaag aactgcattt gtttaccaac gatcatcggg cttacgcagc
    32881 aagcaatagc gtagtcatcg ttgttgaagt ccggacgcat caggtcatcg ttctcatact
    32941 gcagagaaga ggtgtcgatg gacactttag cggcgaattt cttgaagttc agcggcagtt
    33001 tttcagacca cagaatggtc atgttcggtt ccggagacgg acccatggtg tacagggtgt
    33061 tcaggaaacg gaagctgttt ttggtaacca gggtacgacc gtcgaggccc ataccaccga
    33121 tagattcggt tgcccagatc gggtcgccag agaacagttc atcgtattcc ggagtacgca
    33181 ggaagcgaac catacgcagt ttcatgacca ggtggtcaac catttcctgc gcttcttgtt
    33241 cggtgatctt gccagctttc aggtcacgtt cgatgtacac atccaggaag gtggaggtac
    33301 gaccgaagga cattgcagca ccgttctgag acttaacagc agccaggtag ccgaagtaag
    33361 tccactggat agcttcctga gcgttagtag ccggaccaga gatgtcgtag ccgtatttcg
    33421 cagccatttc tttcatctga cccagagcgc ggtgctgttc agcgatttct tcgcgcagac
    33481 ggatagtctg ttccaggttt acgccgtttt ccagatcagc ctgcagagag gtgaactgtg
    33541 caagtttgtc tttcatcagg tagtcgatac cgtacagtgc aacgcgacgg tagtcaccga
    33601 tgatacgacc acggccataa gcatctggca gaccggtcag aacaccggat ttacggcaac
    33661 gcaggatgtc cggagtgtaa acgtcgaaca cgccctggtt gtgagtttta cggtattcag
    33721 tgaagatttt tttgatcatc gggtccagtt cgcggttgta cgctttgcag gaaccttcga
    33781 tcattttgat accaccgaac gggataagag cacgtttcag cggagcttca gtctgcagac
    33841 caacgatttt ctcaagctgc ttgttgatgt aaccagcgtc gtgagaggtg atggtggaag
    33901 caacagcggt gtcaaagtca actggcgcgt gagtgcggtt ttccagttta acgccttcca
    33961 ttactttgtc ccacagggtg gtggtcgctt cagtagcgcc agccaggaag gactcgtcac
    34021 cctcgtacgg agtgtagttt ttctgaatga agtcacggac gtttacttca ttctgccagt
    34081 cacctttggt aaaaccttcc caggctgtgg ctaacttttc attaagctcg gacatgtaac
    34141 acctaccttc ttaagtggat tttttattta ctgcgtactt cgacaaccat taatggtgat
    34201 cgttttcacg caggtaaatg acccagtatg tcaacccaac caacaaacca ccgccgataa
    34261 tattaccgat cgtaaccgga atcaggttat cagtgatgaa gttcatcacg gtcaggtgag
    34321 aaaaattttc cggtgcagaa ccgacagcgg tccagaattc cggagatgcg aagtcgcgga
    34381 ttacaatacc catcgggatc ataaacatgt ttgcgatact gtgctcaaaa ccgctggcaa
    34441 caaacatcgc gaccggcagc accataatga acgctttgtc catcaggctg cggccagaat
    34501 aactcatcca tactgccaga cataccatca ggtttgccag gataccaaga cagacggcct
    34561 caataaaagt atggtgcact ttgtggtcgg cggtttgtag gacgtttagt ccccattgac
    34621 catttgcggt catatactcg ccggaaagcc acattaaaag tacaaacagc agtgcgccga
    34681 ccaggttgcc aaaataaaca tttagccagt ttttcgccaa ctgaccccag gtgatgcgcc
    34741 cactcgcctt ggcaacaaca atcaacactg tggaagtaaa gagatcggct ccgcagacaa
    34801 cacaaagaat caaccccaga gagaagcaaa tgccgccaat cagttttgcc atgccgaagg
    34861 gcattgtacc tgtgccagtg gttgctgtga tatagaagac gaatgcgatt gagatgaaaa
    34921 caccggcggt aatcgccaga tagaaagtct taagcggatg tttcgttgct ttatagacac
    34981 ctgcctcttc ggccactttg gccattgcag caggaagtaa aagatcaaaa gggttgtcag
    35041 ctttcacact aactctctct ttattaagtc ggcgacgaga tactaacaaa gcattataga
    35101 tgagaaatt
//
LOCUS       DAHHTG010000040        35017 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.040, whole genome shotgun sequence.
ACCESSION   DAHHTG010000040 DAHHTG010000000
VERSION     DAHHTG010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 35017)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35017)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35017
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.040"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            187..1842
                     /gene="aslA"
                     /locus_tag="NBI74_001905"
     CDS             187..1842
                     /gene="aslA"
                     /locus_tag="NBI74_001905"
                     /EC_number="3.1.6.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase AslA"
                     /protein_id="HCD1932254.1"
                     /translation="MEFSFSPKLLVVAVAAALPLIASAADTPSTATARKGFAGYDHPN
                     QYLVKPATTIADNMMPVMQHPAQDKETQQKLAELEKKTGKKPNVVVFLLDDVGWMDVG
                     FNGGGVAVGNPTPDIDAVASQGLILTSAYSQPSSSPTRATILTGQYSIHHGILMPPMY
                     GQPGGLQGLTTLPQLLHDQGYVTQAIGKWHMGENKESQPQNVGFDDFRGFNSVSDMYT
                     EWRDVHVNPEVALSPDRSEYIQKLPFSKDDVHAVRGGEQEAIADITPKYMEDLDQRWM
                     EYGVKFLDKMAKSDKPFFLYYGTRGCHFDNYPNAKYAGSSPARTSYGDCMVEMNDIFA
                     NLYKALEKNGQLDNTLIVFTSDNGPEAEVPPHGRTPFRGAKGSTWEGGVRVPTFVYWK
                     GMIQPRKSDGIVDLADLFPTALDLAGHPGAKVANLVPKTTFIDGVDQTSFFLGTNGQS
                     NRKAEHYFLNGKLSAVRMDEFKYHVLIQQPYAYTQSGYQGGFTGTVMQTAGSSVFNLY
                     TDPQESDSIGVRHIPMGVPLQTEMHAYMEILKKYPPRAQIKSD"
     gene            complement(1933..3168)
                     /locus_tag="NBI74_001906"
     CDS             complement(1933..3168)
                     /locus_tag="NBI74_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HCD1932255.1"
                     /translation="MQQQVPTRAFHVMAKPSGSDCNLNCDYCFYLEKQSLYHEKPVTH
                     MDDDTLEAYVRHYIAASETQNEVAFTWQGGEPTLLGLEFYRRAVALQAKYGAGRKISN
                     SFQTNGVLLDDEWCGFLAENHFLVGLSLDGPAEIHNQYRVTKGGRPTHKLVMRALTLL
                     QKHHVDYNVLVCVNRTSALQPLQVYDFLCDAGVEFIQFIPVVERLANETAAHAGLKLH
                     APGDIQGELTEWSVCPQEFGEFLVAIFDHWIKRDVGKIFVMNIEWAFANFVGAPGAVC
                     HHQPTCGRSVIVEHNGDVYACDHYVYPQYRLGNMLQQMIAEMIDSPQQQAFGEDKFKQ
                     LPAQCRSCNVLKACWGGCSKHRFMLDASGKPGLNYLCAGYQRYFRHLPPYLKAMVDLL
                     AHGRPASDIMQAHLLVVNK"
     gene            complement(3315..3391)
                     /locus_tag="NBI74_001907"
     tRNA            complement(3315..3391)
                     /locus_tag="NBI74_001907"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3355..3357),aa:Pro,seq:tgg)
     gene            complement(3434..3520)
                     /locus_tag="NBI74_001908"
     tRNA            complement(3434..3520)
                     /locus_tag="NBI74_001908"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3484..3486),aa:Leu,seq:cag)
     gene            complement(3541..3616)
                     /locus_tag="NBI74_001909"
     tRNA            complement(3541..3616)
                     /locus_tag="NBI74_001909"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3581..3583),aa:His,seq:gtg)
     gene            complement(3675..3751)
                     /locus_tag="NBI74_001910"
     tRNA            complement(3675..3751)
                     /locus_tag="NBI74_001910"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3715..3717),aa:Arg,seq:ccg)
     gene            complement(3854..5239)
                     /locus_tag="NBI74_001911"
     CDS             complement(3854..5239)
                     /locus_tag="NBI74_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCD1932256.1"
                     /translation="MADNKPELQRGLEARHIELIALGGTIGVGLFMGAASTLKWAGPS
                     VLLAYIIAGLFVFFIMRSMGEMLFLEPVTGSFAVYAHRYMSPFFGYLTAWSYWFMWMA
                     VGISEITAIGVYVQFWFPEMAQWIPALIAVALVALANLAAVRLYGEIEFWFAMIKVTT
                     IIVMIVIGLGVIFFGFGNGGQSIGFSNLTEHGGFFAGGWKGFLTALCIVVASYQGVEL
                     IGITAGEAKNPQVTLRSAVGKVLWRILIFYVGAIFVIVTIFPWNEIGSNGSPFVLTFA
                     KIGITAAAGIINFVVLTAALSGCNSGMYSCGRMLYALAKNRQLPAAMAKVSRHGVPVA
                     GVAVSIAILLIGSCLNYIIPNPQRVFVYVYSASVLPGMVPWFVILISQLRFRRAHKAA
                     IASHPFRSILFPWANYVTMAFLICVLIGMYFNEDTRMSLFVGIIFMLTVTAIYKVFGL
                     NRHGKAHKLEE"
     gene            complement(5430..6170)
                     /gene="rffM"
                     /locus_tag="NBI74_001912"
     CDS             complement(5430..6170)
                     /gene="rffM"
                     /locus_tag="NBI74_001912"
                     /EC_number="2.4.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     N-acetylmannosaminouronosyltransferase"
                     /protein_id="HCD1932257.1"
                     /translation="MNNNTTAPTYTLRGLQLIGWRDMQHALDYLFADGQLKQGTLVAI
                     NAEKMLTIEDNAEVRELINAAEFKYADGISVVRSVRKKYPQAQVSRVAGADLWEELMA
                     RAGKEGTPVFLVGGKPEVLAQTEAKLRNQWNVNIVGSQDGYFKPEQRQALFERIHASG
                     AQIVTVAMGSPKQEIFMRDCRLVHPDALYMGVGGTYDVFTGHVKRAPKIWQTLGLEWL
                     YRLLSQPSRIKRQLRLLRYLRWHYTGNL"
     gene            complement(6173..7525)
                     /gene="wzyE"
                     /locus_tag="NBI74_001913"
     CDS             complement(6173..7525)
                     /gene="wzyE"
                     /locus_tag="NBI74_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECA oligosaccharide polymerase"
                     /protein_id="HCD1932258.1"
                     /translation="MSLLQFSGLFVVWLLCTLFIATLTWFEFRRVRFNFNVFFSLLFL
                     LTFFFGFPLTSVLVFRFDVGAAPPEILLQALLSAGCFYAVYYVTYKTRLRKRVADVPR
                     RPLFTMNRVETNLTWVILMGIALVSVGIFFMHNGFLLFRLNSYSQIFSSEVSGVALKR
                     FFYFFIPAMLVVYFLRQDSKAWLFFLVSTVAFGLLTYMIVGGTRANIIIAFAIFLFIG
                     IIRGWISLWMLAAAGVLGIVGMFWLALKRYGMNVSGDEAFYTFLYLTRDTFSPWENLA
                     LLLQNYDNIDFQGLAPIVRDFYVFIPSWLWPGRPSMVLNSANYFTWEVLNNHSGLAIS
                     PTLIGSLVVMGGALFIPLGAIVVGLIIKWFDWLYELGNRETNRYKAAILHSFCFGAIF
                     NMIVLAREGLDSFVSRVVFFIVVFGACLMIAKLLYWLFESAGLIHKRTKSSLRTQVEG
                     "
     gene            complement(7522..8601)
                     /gene="rffT"
                     /locus_tag="NBI74_001914"
                     /gene_synonym="wecF"
     CDS             complement(7522..8601)
                     /gene="rffT"
                     /locus_tag="NBI74_001914"
                     /gene_synonym="wecF"
                     /EC_number="2.4.1.325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDP-N-acetylfucosamine:lipid II
                     N-acetylfucosaminyltransferase"
                     /protein_id="HCD1932259.1"
                     /translation="MTVLIHVLGSDIPHHNRTVLRFFNDALAATSEHAREFMVVGKDD
                     GLSDSCPALSVQFFPGKKSLAEAVIAKAKANRQQRFFFHGQFNPTLWLALLSGGIKPS
                     QFYWHIWGADLYELSSGLRYKLFYPLRRLAQKRVGCVFATRGDLSFFAKTHPKVRGEL
                     LYFPTRMDPSLNMMANDRQREGKMTILVGNSGDRSNEHIAALRAVYQQFGDTVKVVVP
                     MGYPPNNEAYIEEVRQAGLELFSEENLQTLSEKLEFDAYLALLRQCDLGYFIFARQQG
                     IGTLCLLIQAGIPCVLNRENPFWQDMTEQHLPVLFTTDDLNEDIVREAQRQLVSVDKN
                     TIAFFSPNYLQGWQRALAIAAGEVA"
     gene            complement(8598..9848)
                     /gene="wzxE"
                     /locus_tag="NBI74_001915"
     CDS             complement(8598..9848)
                     /gene="wzxE"
                     /locus_tag="NBI74_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid III flippase WzxE"
                     /protein_id="HCD1932260.1"
                     /translation="MSLAKASLWTAASTLVKIGAGLLVGKLLAVSFGPAGLGLAANFR
                     QLITVLGVLAGAGIFNGVTKYVAQYHDNPQQLRRVVGTSSAMVLGFSTLMALVFVLAA
                     APISQGLFGNTDYQGLVRLVALVQMGIAWGNLLLALMKGFRDAAGNALSLIVGSLIGV
                     LAYYVSYRLGGYEGALLGLALIPALVVIPAAIMLIKRGVIPLSYLKPSWDNGLAGQLS
                     KFTLMALITSVTLPVAYIMMRKLLAAQYSWDEVGIWQGVSSISDAYLQFITASFSVYL
                     LPTLSRLTEKRDITREVVKSLKFVLPAVAAASFTVWLLRDFAIWLLLSNKFTAMRDLF
                     AWQLVGDVLKVGAYVFGYLVIAKASLRFYILAEVSQFTLLMVFAHWLIPAHGALGAAQ
                     AYMATYIVYFSLCCGVFLLWRRRA"
     gene            complement(9850..10980)
                     /gene="rffA"
                     /locus_tag="NBI74_001916"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
     CDS             complement(9850..10980)
                     /gene="rffA"
                     /locus_tag="NBI74_001916"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
                     /EC_number="2.6.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxygalactose transaminase"
                     /protein_id="HCD1932261.1"
                     /translation="MIPFNAPPVVGTELDYMQSAMGSGKLCGDGGFTRRCQQWLEQRF
                     GSAKVLLTPSCTASLEMAALLLDIQPGDEVIMPSYTFVSTANAFVLRGAKIVFVDVRP
                     DTMNINETLIEAAITDKTRVIVPVHYAGVACEMDTIMALAKKHNLFVVEDAAQGVMST
                     YKGRALGTIGHIGCFSFHETKNYTAGGEGGATLINDKALIERAEIIREKGTNRSQFFR
                     GQVDKYTWRDIGSSYLMSDLQAAYLWAQLEAADRINQQRLALWQNYYDALAPLAKNGR
                     IELPSIPDGCVQNAHMFYIKLRDIDDRSALINFLKEAEIMAVFHYIPLHGCPAGEHFG
                     EFHGEDRYTTKESERLLRLPLFYNLSPVNQRTVIATLLNYFS"
     gene            complement(10985..11659)
                     /gene="rffC"
                     /locus_tag="NBI74_001917"
                     /gene_synonym="wecD"
     CDS             complement(10985..11659)
                     /gene="rffC"
                     /locus_tag="NBI74_001917"
                     /gene_synonym="wecD"
                     /EC_number="2.3.1.210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxy-D-galactose
                     acyltransferase"
                     /protein_id="HCD1932262.1"
                     /translation="MPVRASIEPLTWENAFFGVNSAIVRISPDAALLTPDALAPWSRV
                     QAKIAASNTGELDALQQLGFSLIEGEVDLALPVNNVSDSGAVVAQETDIPALRQLASA
                     AFAQSRFRAPWYAPDASGRFYAQWIENAVRGTFDHQCLILRAASGDIRGYVSLRELNA
                     TDARIGLLAGRGAGAELMQTALNWAYARGKTTLRVATQMGNTAALKRYIQSGANVEST
                     AYWLYR"
     gene            complement(11637..12518)
                     /gene="rfbA"
                     /locus_tag="NBI74_001918"
     CDS             complement(11637..12518)
                     /gene="rfbA"
                     /locus_tag="NBI74_001918"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HCD1932263.1"
                     /translation="MKGIILAGGSGTRLHPITRGVSKQLLPIYDKPMIYYPLSVLMLA
                     GIREILIITTPEDKGYFQRLLGDGSEFGIQLEYAEQPSPDGLAQAFIIGETFLNGEPS
                     CLVLGDNIFFGQGFSPKLRHVAARTEGATVFGYQVMDPERFGVVEFDDNFRAISLEEK
                     PKQPKSNWAVTGLYFYDSKVVEYAKQVKPSERGELEITSINQMYLEAGNLTVELLGRG
                     FAWLDTGTHDSLIEASTFVQTVEKRQGFKIACLEEIAWRNGWLDDEGVKRAASSLAKT
                     GYGQYLLELLRARPRQY"
     gene            complement(12537..13604)
                     /gene="rffG"
                     /locus_tag="NBI74_001919"
     CDS             complement(12537..13604)
                     /gene="rffG"
                     /locus_tag="NBI74_001919"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HCD1932264.1"
                     /translation="MRKILITGGAGFIGSALVRYIINETSDAVVVVDTLTYAGNLMSL
                     APVAQSERFAFEKVDICDRAELARVFTEYQPDCVMHLAAESHVDRSIDGPAAFIETNI
                     VGTYTLLEAARAYWTALTEDKKSAFRFHHISTDEVYGDLHSTDDFFTETTPYAPSSPY
                     SASKASSDHLVRAWLRTYGLPTLITNCSNNYGPYHFPEKLIPLMILNALAGKPLPVYG
                     NGQQIRDWLYVEDHARALYCVATTGKVGETYNIGGHNERKNLDVVETICELLEELAPN
                     KPHGVAHYRDLITFVADRPGHDLRYAIDASKIARELGWLPQETFESGMRKTVQWYLAN
                     ESWWKQVQDGSYQGERLGLKG"
     gene            complement(13604..14866)
                     /gene="wecC"
                     /locus_tag="NBI74_001920"
     CDS             complement(13604..14866)
                     /gene="wecC"
                     /locus_tag="NBI74_001920"
                     /EC_number="1.1.1.336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetyl-D-mannosamine dehydrogenase"
                     /protein_id="HCD1932265.1"
                     /translation="MSFATISVIGLGYIGLPTAAAFASRQKQVIGVDINQHAVDTINR
                     GEIHIVEPDLASVVKTAVEGGFLRASTTPVEADAWLIAVPTPFKGDHEPDMTYVESAA
                     RSIAPVLKKGALVILESTSPVGSTEKMAEWLAEMRPDLTFPQQVGELADVNIAYCPER
                     VLPGQVMVELIKNDRVIGGMTPVCSARASELYKIFLEGECVVTNSRTAEMCKLTENSF
                     RDVNIAFANELSLICADQGINVWELIRLANRHPRVNILQPGPGVGGHCIAVDPWFIVA
                     QNPQQARLIRTAREVNDHKPFWVIDQVKAAVADCLAATDKRANELKIACFGLAFKPNI
                     DDLRESPAMEIAELIAQWHSGETLVVEPNIHQLPKKLTGLCTLAQLDEALATADVLVM
                     LVDHSQFKVINGDNVHQQYVVDAKGVWR"
     gene            complement(14863..15993)
                     /gene="wecB"
                     /locus_tag="NBI74_001921"
     CDS             complement(14863..15993)
                     /gene="wecB"
                     /locus_tag="NBI74_001921"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HCD1932266.1"
                     /translation="MKVLTVFGTRPEAIKMAPLVHALAKDPFFEAKVCVTAQHREMLD
                     QVLKLFSIVPDYDLNIMQPGQGLTEITCRILEGLKPILAEFKPDVVLVHGDTTTTLAT
                     SLAAFYQRIPVGHVEAGLRTGDLYSPWPEEANRTLTGHLAMYHFSPTETSRQNLLREN
                     VADSRIFITGNTVIDALLWVRDQVMSSDTLRSELAANYPFIDPDKKMILVTGHRRESF
                     GRGFEEICHALADIATTHQDIQIVYPVHLNPNVREPVNRILGHVKNVILIDPQEYLPF
                     VWLMNHAWLILTDSGGIQEEAPSLGKPVLVMRDTTERPEAVTAGTVRLVGTDKQRIVE
                     EVTRLLKDENEYQAMSRAHNPYGDGQACSRILEALKNNRISL"
     gene            complement(16049..17095)
                     /gene="wzzE"
                     /locus_tag="NBI74_001922"
     CDS             complement(16049..17095)
                     /gene="wzzE"
                     /locus_tag="NBI74_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECA polysaccharide chain length modulation
                     protein"
                     /protein_id="HCD1932267.1"
                     /translation="MTQPMPGKPAEDAENELDIRGLFRTLWAGKLWIIGMGLAFALIA
                     LAYTFFARQEWSATAITDRPTVNMLGGYYSQQQFLRNLDVRSNMASADQPSVMDEAYK
                     EFVMQLASWDTRREFWLQTDYYKQRMVGNSKADAALLDEMINNIVFIPGDFTRAVNDS
                     VKLIAETAPDANNLLRQYVAFASQRAASHLNDELKGAWAARTIQMKAQVKRQEEVAKA
                     IYDRRMNSIEQALKIAEQHNISRSATDVPAEELPDSEMFLLGRPMLQARLENLQAVGP
                     AFDLDYDQNRAMLNTLNVGPTLDPRFQTYRYLRTPEEPVKRDSPRRAFLMIMWGIVGG
                     LIGAGVALTRRCSK"
     gene            complement(17107..18210)
                     /gene="wecA"
                     /locus_tag="NBI74_001923"
     CDS             complement(17107..18210)
                     /gene="wecA"
                     /locus_tag="NBI74_001923"
                     /EC_number="2.7.8.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--undecaprenyl-phosphate
                     N-acetylglucosaminephosphotransferase"
                     /protein_id="HCD1932268.1"
                     /translation="MNLLTVSTDLISIFLFTTLFLFFARKVAKKVGLVDKPNFRKRHQ
                     GLIPLVGGISVYAGICFTFGIVDYYIPQASLYLACAGVLVFIGALDDRFDISVKIRAT
                     IQAAVGIVMMVFGKLYLSSLGYIFGSWEMVLGPFGYFLTLFAVWAAINAFNMVDGIDG
                     LLGGLSCVSFAAIGMILWFDGQTSLAIWCFAMIAAILPYIMLNLGILGRRYKVFMGDA
                     GSTLIGFTVIWILLETTQGKTHPISPVTALWIIAIPLMDMVAIMYRRLRKGMSPFSPD
                     RQHIHHLIMRAGFTSRQAFVLITLAAALLASIGVLAEYSHFVPEWVMLVLFLLAFFLY
                     GYCIKRAWKVARFIKRVKRRLRRNRGGSPNLTK"
     gene            complement(18450..19709)
                     /gene="rho"
                     /locus_tag="NBI74_001924"
     CDS             complement(18450..19709)
                     /gene="rho"
                     /locus_tag="NBI74_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017492510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HCD1932269.1"
                     /translation="MNLTELKNTPVSELITLGENMGLENLARMRKQDIIFAILKQHAK
                     SGEDIFGDGVLEILQDGFGFLRSADSSYLAGPDDIYVSPSQIRRFNLRTGDTISGKIR
                     PPKEGERYFALLKVNEVNFDKPENARNKILFENLTPLHANSRLRMERGNGSTEDLTAR
                     VLDLASPIGRGQRGLIVAPPKAGKTMLLQNIAQSIAYNHPDCVLMVLLIDERPEEVTE
                     MQRLVKGEVVASTFDEPASRHVQVAEMVIEKAKRLVEHKKDVIILLDSITRLARAYNT
                     VVPASGKVLTGGVDANALHRPKRFFGAARNVEEGGSLTIIATALIDTGSKMDEVIYEE
                     FKGTGNMELHLSRKIAEKRVFPAIDYNRSGTRKEELLTTQEELQKMWILRKIIHPMGE
                     IDAMEFLINKLAMTKTNDDFFEMMKRS"
     gene            complement(19794..19895)
                     /gene="rhoL"
                     /locus_tag="NBI74_001925"
     CDS             complement(19794..19895)
                     /gene="rhoL"
                     /locus_tag="NBI74_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rho operon leader peptide RhoL"
                     /protein_id="HCD1932270.1"
                     /translation="MRSEQISGSSLNPSCRFSSAYSPVTRQRKDMSR"
     gene            complement(20036..20365)
                     /gene="trxA"
                     /locus_tag="NBI74_001926"
     CDS             complement(20036..20365)
                     /gene="trxA"
                     /locus_tag="NBI74_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017375425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxA"
                     /protein_id="HCD1932271.1"
                     /translation="MSDKIIHLTDDSFDTDVLKADGAILVDFWAEWCGPCKMIAPILD
                     EIADEYQGKLTVAKLNIDQNPGTAPKYGIRGIPTLLLFKNGEVAATKVGALSKGQLKE
                     FLDANLA"
     gene            20496..21761
                     /gene="rhlB"
                     /locus_tag="NBI74_001927"
     CDS             20496..21761
                     /gene="rhlB"
                     /locus_tag="NBI74_001927"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlB"
                     /protein_id="HCD1932272.1"
                     /translation="MSKTHLTEQKFSDFALHPKVVEALEKKGFHNCTPIQALALPLTL
                     AGRDVAGQAQTGTGKTMAFLTSTFHYLLSHPAIADRKVNQPRALIMAPTRELAVQIHA
                     DAEPLAEATGLKLGLAYGGDGYDKQLKVLESGVDILIGTTGRLIDYAKQNHINLGAIQ
                     VVVLDEADRMYDLGFIKDIRWLFRRMPPANQRLNMLFSATLSYRVRELAFEQMNNAEY
                     IEVEPEQKTGHRIKEELFYPSNEEKMRLLQTLIEEEWPDRAIIFANTKHRCEEIWGHL
                     AADGHRVGLLTGDVAQKKRLRILDEFTRGDLDILVATDVAARGLHIPAVTHVFNYDLP
                     DDCEDYVHRIGRTGRAGASGHSISLACEEYALNLPAIETYIGHSIPVSKYNPDALMTD
                     LPKPLRLTRPRTGNGPRRTGAPRNRRRSG"
     gene            21897..23381
                     /gene="gppA"
                     /locus_tag="NBI74_001928"
     CDS             21897..23381
                     /gene="gppA"
                     /locus_tag="NBI74_001928"
                     /EC_number="3.6.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanosine-5'-triphosphate,3'-diphosphate
                     diphosphatase"
                     /protein_id="HCD1932273.1"
                     /translation="MGSTSSLYAAIDLGSNSFHMLVVREVAGSIQTLTRIKRKVRLAA
                     GLNSENALSNEAMERGWQCLRLFAERLQDIPPSQIRVVATATLRLAVNAGDFIAKAQE
                     ILGCPVQVISGEEEARLIYQGVAHTTGGADQRLVVDIGGASTELVTGTGAQTTSLFSL
                     SMGCVTWLERYFADRNLGQENFDAAEKAAREVLRPVADELRYHGWKVCVGASGTVQAL
                     QEIMMAQGMDERITLEKLQQLKQRAIHCGRLEELEIDGLTLERALVFPSGLAILIAIF
                     TELNIQCMTLAGGALREGLVYGMLHLTVEQDIRSRTLRNIQRRFMIDIDQAQRVTKVA
                     ANFFDQVENEWHLEAISRDLLISACQLHEIGLSVDFKQAPQHAAYLVRNLDLPGFTPA
                     QKKLLATLLLNQTNPVDLSSLHQQNAVPPRVAEQLCRLLRLAIIFASRRRDDLVPEMT
                     LQANHELLTLTLPQGWLTQHPLGKEIIVQESQWQSYVHWPLEVH"
     gene            complement(23428..25449)
                     /gene="rep"
                     /locus_tag="NBI74_001929"
     CDS             complement(23428..25449)
                     /gene="rep"
                     /locus_tag="NBI74_001929"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase Rep"
                     /protein_id="HCD1932274.1"
                     /translation="MRLNPGQQQAVEFVTGPCLVLAGAGSGKTRVITNKIAHLIRSCG
                     YQARHIAAVTFTNKAAREMKERVGQTLGRKEARGLMISTFHTLGLDIIKREYAALGMK
                     ANFSLFDDTDQLALLKELTEGLIEDDKVLLQQLISTISNWKNDLKTPAQAAAEAKGER
                     DRIFAHCYGLYDAHLKACNVLDFDDLILLPTLLLQRNEEVRERWQNKIRYLLVDEYQD
                     TNTSQYELVKLLVGSRARFTVVGDDDQSIYSWRGARPQNLVLLSQDFPALKVIKLEQN
                     YRSSGRILKAANILIANNPHVFEKRLFSELGYGTELKVLSANNEEHEAERVTGELIAH
                     HFVNKTQYKDYAILYRGNHQSRVFEKFLMQNRIPYKISGGTSFFSRPEIKDLLAYLRV
                     LTNPDDDSAFLRIVNTPKREIGPATLKKLGEWAMTRNKSMFTASFDMGLSQTLSGRGY
                     EALTRFTHWLAEIQRLAEREPIAAVRDLIHGMDYESWLYETSPSPKAAEMRMKNVNQL
                     FSWMTEMLEGSELDEPMTLTQVVTRFTLRDMMERGESEEELDQVQLMTLHASKGLEFP
                     YVYMVGMEEGFLPHQSSIDEDNIDEERRLAYVGITRAQKELTFTLCKERRQYGELVRP
                     EPSRFLLELPQDDLIWEQERKVVSAEERMQKGQSHLANLKAMMAAKRGK"
     gene            25536..25817
                     /gene="ppiC"
                     /locus_tag="NBI74_001930"
     CDS             25536..25817
                     /gene="ppiC"
                     /locus_tag="NBI74_001930"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004097481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase PpiC"
                     /protein_id="HCD1932275.1"
                     /translation="MAKTAAALHILVKEEKLALDLLEQIKNGADFGKLAKKHSICPSG
                     KRGGDLGEFRQGQMVPAFDKVVFSCPVLEPTGPLHTQFGYHIIKVLYRN"
     gene            complement(25863..27338)
                     /gene="ilvC"
                     /locus_tag="NBI74_001931"
     CDS             complement(25863..27338)
                     /gene="ilvC"
                     /locus_tag="NBI74_001931"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HCD1932276.1"
                     /translation="MANYFNTLNLRQQLAQLGKCRFMGRDEFADGASYLQGKKVVIVG
                     CGAQGLNQGLNMRDSGLDISYALRKEAIAEKRASWRKATENGFKVGTYEELIPQADLV
                     VNLTPDKQHSDVVRSVQPLMKDGAALGYSHGFNIVEVGEQIRKDITVVMVAPKCPGTE
                     VREEYKRGFGVPTLIAVHPENDPKGEGMAIAKAWAAATGGHRAGVLESSFVAEVKSDL
                     MGEQTILCGMLQAGSLLCFDKLVEEGTDPAYAEKLIQFGWETITEALKQGGITLMMDR
                     LSNPAKLRAYALSEQLKEIMAPLFQKHMDDIISGEFSSGMMADWANDDKKLLTWREET
                     GKTAFETAPQYEGKIGEQEYFDKGVLMIAMVKAGVELAFETMVDSGIIEESAYYESLH
                     ELPLIANTIARKRLYEMNVVISDTAEYGNYLFSYACVPLLKPFMAELQPGDLGKAIPE
                     GAVDNAQLRDVNEAIRSHAIEQVGKKLRGYMTDMKRIAVAG"
     gene            27488..28378
                     /gene="ilvY"
                     /locus_tag="NBI74_001932"
     CDS             27488..28378
                     /gene="ilvY"
                     /locus_tag="NBI74_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator IlvY"
                     /protein_id="HCD1932277.1"
                     /translation="MDLRDLKTFLHLAESRHFGRSARAMHVSPSTLSRQIQRLEDDLG
                     QPLFVRDNRTVTLTEAGEELRVFAQQTLLQYQQLRHTIDQQGPSLSGELHIFCSVTAA
                     YSHLPPILDRFRAEHPSVEIKLTTGDAADAMEKVVTGEADLAIAGKPETLPGAVAFSM
                     LENLAVVLIAPALPCPVRNQVSAEKPDWSTVPFIMADQGPVRRRIELWFRRNKISNPM
                     IYATVGGHEAMVSMVALGCGVALLPEVVLENSPEPVRNRVMILERSDEKTPFELGVCV
                     QKKRLHEPLIEAFWKILPNH"
     gene            complement(28375..29919)
                     /gene="ilvA"
                     /locus_tag="NBI74_001933"
     CDS             complement(28375..29919)
                     /gene="ilvA"
                     /locus_tag="NBI74_001933"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HCD1932278.1"
                     /translation="MADSQPLSGAPEGAEYLRAVLRAPVYEAAQVTPLQKMEKLSSRL
                     DNVILVKREDRQPVHSFKLRGAYAMMAGLTEEQKAHGVITASAGNHAQGVAFSSARLG
                     VKALIVMPTATADIKVDAVRGFGGEVLLHGANFDEAKAKAIELSQQQGFTWVPPFDHP
                     MVIAGQGTLALELLQQDAHLDRVFVPVGGGGLAAGVAVLIKQLMPQIKVIAVEAEDSA
                     CLKAALDAGHPVDLPRVGLFAEGVAVKRIGDETFRLCQEYLDDIITVDSDAICAAMKD
                     LFEDVRAVAEPSGALALAGMKKYIAQHNIRGERLAHILSGANVNFHGLRYVSERCELG
                     EQREALLAVTIPEEKGSFLKFCQLLGGRSVTEFNYRFADAKNACIFVGVRLSRGLEER
                     KEILQMLNDGGYSVVDLSDDEMAKLHVRYMVGGRPSHPLQERLYSFEFPESPGALLRF
                     LNTLGTHWNISLFHYRSHGTDYGRVLAAFELGDHEPDFETRLNELGYDCHDETNNPAF
                     RFFLAG"
     gene            complement(29922..31772)
                     /gene="ilvD"
                     /locus_tag="NBI74_001934"
     CDS             complement(29922..31772)
                     /gene="ilvD"
                     /locus_tag="NBI74_001934"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HCD1932279.1"
                     /translation="MPKYRSATTTHGRNMAGARALWRATGMTDADFGKPIIAVVNSFT
                     QFVPGHVHLRDLGKLVAEQIEAAGGVAKEFNTIAVDDGIAMGHGGMLYSLPSRELIAD
                     SVEYMVNAHCADAMVCISNCDKITPGMLMASLRLNIPVIFVSGGPMEAGKTKLSDQII
                     KLDLVDAMIQGADPKVSDSQSDQVERSACPTCGSCSGMFTANSMNCLTEALGLSQPGN
                     GSLLATHADRKQLFLNAGKRIVELTKRYYEQDDESALPRNIASKAAFENAMTLDIAMG
                     GSTNTVLHLLAAAQEAEIDFTMSDIDKLSRKVPQLCKVAPSTQKYHMEDVHRAGGVIG
                     ILGELDRAGLLNRDVKNVLGLTLPQTLEQYDIIVTQDDAVKNMFRAGPAGIRTTQAFS
                     QNCRWDTLDDDRSNGCIRSLEHAYSKDGGLAVLYGNFAENGCIVKTAGVDDSILKFTG
                     PAKVYESQDDAVEAILGGKVVAGDVVVIRYEGPKGGPGMQEMLYPTSFLKSMGLGKAC
                     ALITDGRFSGGTSGLSIGHVSPEAASGGSIGLIEDGDLIAIDIPNRGIQLQVSDAELA
                     ARREAQEARGNKAWTPKNRERQVSFALRAYASLATSADKGAVRDKSKLGG"
     gene            complement(31837..32766)
                     /gene="ilvE"
                     /locus_tag="NBI74_001935"
     CDS             complement(31837..32766)
                     /gene="ilvE"
                     /locus_tag="NBI74_001935"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain-amino-acid transaminase"
                     /protein_id="HCD1932280.1"
                     /translation="MTTKKADYIWFNGEMVRWEDAKVHVMSHALHYGTSVFEGIRCYD
                     SHKGPVVFRHREHMQRLHDSAKIYRFPVSQSIDELMEACRDVIRKNNLTSAYIRPLIF
                     VGDVGMGVNPPAGYSTDVIIAAFPWGAYLGAEALEQGIDAMVSSWNRAAPNTIPTAAK
                     AGGNYLSSLLVGSEARRHGYQEGIALDVNGYISEGAGENLFEVKDGVLFTPPFTSSAL
                     PGITRDAIIKLAKELGIEVREQVLSRESLYLADEVFMSGTAAEITPVRSVDGIQVGEG
                     RCGPVTKRIQQAFFGLFTGETEDKWGWLDQVNQ"
     gene            complement(32786..33049)
                     /gene="ilvM"
                     /locus_tag="NBI74_001936"
     CDS             complement(32786..33049)
                     /gene="ilvM"
                     /locus_tag="NBI74_001936"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 small subunit"
                     /protein_id="HCD1932281.1"
                     /translation="MMQHQVNVSARFNPETLERVLRVVRHRGFHVCSMNMAAASDAQN
                     INIELTVASPRSVDLLFSQLNKLVDVAHVAICQSTTTSQQIRA"
     gene            complement(33046..34692)
                     /locus_tag="NBI74_001937"
     CDS             complement(33046..34692)
                     /locus_tag="NBI74_001937"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001012636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 catalytic subunit"
                     /protein_id="HCD1932282.1"
                     /translation="MNGAQWVVHALRAQGVNTVFGYPGGAIMPVYDALYDGGVEHLLC
                     RHEQGAAMAAIGYARATGKTGVCIATSGPGATNLITGLADALLDSIPVVAITGQVSAA
                     FIGTDAFQEVDILGLSLACTKHSFLVQSLEELPRIMAEAFDVASSGRPGPVLVDIPKD
                     IQLASGDLEPWFTTVENEVTFPHAEVEQARQMLAKAQKPMLYVGGGVGMAQAVSALRE
                     FLAATKMPATCTLKGLGAVEADYPYYLGMLGMHGTKAANFAVQECDLLIAVGARFDDR
                     VTGKLNTFAPHASVIHMDIDPAEMNKLRQAHVALQGDLNALLPALQQPLNIDDWQQHC
                     AQLRDEHAWRYDHPGDAIYAPLLLKQLSDRKPADCIVTTDVGQHQMWAAQHIVHTRPE
                     NFITSSGLGTMGFGLPAAVGAQVARPNDTVVCISGDGSFMMNVQELGTVKRKQLPLKI
                     VLLDNQRLGMVRQWQQLFFQERYSETTLTDNPDFLMLASAFGIPGQHITHKDQVEAAL
                     DTMLNSDGPYLLHVSIDELENVWPLVPPGASNSEMLEKLS"
     gene            complement(34695..34745)
                     /locus_tag="NBI74_001938"
     CDS             complement(34695..34745)
                     /locus_tag="NBI74_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1932283.1"
                     /translation="MNNSTKFCFSRFRTGN"
     gene            complement(34832..34930)
                     /gene="ilvL"
                     /locus_tag="NBI74_001939"
     CDS             complement(34832..34930)
                     /gene="ilvL"
                     /locus_tag="NBI74_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ilv operon leader peptide"
                     /protein_id="HCD1932284.1"
                     /translation="MTALLRVISLVVISVVVIIIPPCGAALGRGKA"
BASE COUNT         8470 a         9670 c         8861 g         8016 t
ORIGIN      
        1 acatatcacc tgcgccagag gtaggattga aaacgctctc ctgattttcc aattcatttt
       61 ctggataaat aaataattta tttttatcac cattatttat gcaatcatcc tgtcagggag
      121 agggatctca attatcaatg cttaattacg tcatcatttt gacgacatga aaccggagca
      181 ttttgcatgg aattttcgtt ttcacccaaa cttcttgttg ttgctgtcgc cgcagctctt
      241 cctctcatag ccagcgcagc agataccccg tcaaccgcca ccgcacgcaa aggctttgcc
      301 ggatacgatc acccaaacca gtatctggtt aaaccggcga ccactattgc cgacaacatg
      361 atgccggtga tgcagcatcc tgcacaagac aaagaaaccc agcagaaact ggcggaactt
      421 gagaaaaaaa ccggtaagaa accgaacgtg gttgttttct tgctggatga tgtgggctgg
      481 atggacgttg gctttaacgg cggcggcgtg gcggtgggta accctacacc agatatcgac
      541 gccgttgcca gccaggggct gattttaact tcggcgtatt ctcaaccgag ttcttcccca
      601 acccgcgcca cgattctcac cggacaatac tccatccacc acggcattct gatgccgcca
      661 atgtacgggc aaccgggcgg tctgcaaggg ttaaccacgc tgccacagtt gctgcacgat
      721 cagggctacg tcacccaggc tatcgggaaa tggcatatgg gggaaaacaa agagtcgcag
      781 ccgcagaacg ttggctttga tgatttccgc ggctttaact cggtatctga tatgtatacc
      841 gaatggcgtg atgttcatgt caacccggaa gtcgccctga gcccggatcg ttctgaatac
      901 atccaaaaac tgccgtttag caaagatgac gttcatgccg tgcgcggcgg tgaacaggaa
      961 gccattgccg acattacgcc gaaatatatg gaagatctgg atcaacgctg gatggaatat
     1021 ggcgttaagt tcctcgacaa gatggcgaag agtgataagc ctttcttcct ctattacggc
     1081 actcgcggct gtcacttcga taactacccg aacgccaaat atgcgggtag ctctccggcg
     1141 cgcacctctt acggcgactg catggtggag atgaacgata tcttcgccaa tctgtataaa
     1201 gcactggaga aaaacggtca gcttgataac acgctgattg tgttcacctc tgataacggt
     1261 ccggaagccg aagttccgcc gcacggtcgc acgccgttcc gcggtgcgaa aggttctaca
     1321 tgggaaggcg gcgttcgtgt gccaaccttc gtttactgga aaggcatgat ccaaccgcgt
     1381 aaatctgacg gcattgtcga tctggcggat ctcttcccta cggcgctgga tctggcgggg
     1441 catcctgggg cgaaagtagc gaatttagta ccgaaaacca cctttatcga tggtgtggac
     1501 cagacatctt tcttcctggg aacaaatggt cagtctaacc gtaaggccga gcattacttc
     1561 ctcaacggta aactttctgc tgtgcgtatg gatgagttca agtatcacgt cctgattcag
     1621 caaccttacg cttataccca gagcggatat cagggtggat tcaccggcac agtaatgcaa
     1681 acggcgggat cgtcggtgtt taacctgtac accgatccgc aggaaagcga ctctatcggc
     1741 gtgcgccata tcccaatggg tgtaccgcta cagaccgaaa tgcatgcgta tatggagatc
     1801 ctgaaaaaat atccaccacg cgcgcagatt aaatctgatt aagccggcgc tatcgctgag
     1861 atctaccttt gccggatgcg atgctgacgc atccgacaat caaccgcagg cggccacaaa
     1921 atggctgctt ttttacttat tcaccaccag caaatgcgcc tgcataatgt cgctggccgg
     1981 acgaccgtgc gccagcaaat caaccattgc tttaagatat ggcggtagat ggcggaaata
     2041 acgctgatac ccggcacaca aataattcag cccgggtttg ccgctggcat cgagcataaa
     2101 gcggtgtttc gagcagcctc cccagcacgc ttttaacacg ttgcaactgc gacactgtgc
     2161 cggtaactgc ttgaatttat cttcaccaaa tgcctgctgt tgcggggaat caatcatttc
     2221 tgcaatcatc tgctgaagca tattccctag tcgatactgc ggataaacat agtgatcgca
     2281 ggcataaacg tcaccgttgt gctcaacaat caccgagcgc ccacaggttg gctgatgatg
     2341 gcaaaccgcg cccggtgcgc cgacaaaatt ggcgaacgcc cattcgatat tcattacgaa
     2401 aatcttgccg acgtcgcgct taatccagtg gtcgaaaatc gccaccagaa actcaccgaa
     2461 ctcttgcggg cacaccgacc attccgtcag ttcgccctga atatcgccgg gagcatgtag
     2521 cttaagtcca gcatgagcag ccgtttcatt agccaggcgc tcgaccaccg gaataaactg
     2581 gatgaactca acacctgcat cacacagaaa atcatagact tgcaaaggtt gtaacgcact
     2641 ggtgcgattg acgcagacca gcacgttata gtcgacatga tgtttttgca ggagcgtcag
     2701 ggcacgcatc accagcttat gcgtgggtct gccacctttg gtcacgcgat attgattgtg
     2761 gatctcagcc ggaccatcca gcgataaccc tacaagaaaa tgattttctg ccagaaatcc
     2821 acaccattcg tcatcgagca gcacgccgtt agtctggaag ctattactta tcttcctgcc
     2881 agcaccatat ttcgcctgta gcgccacggc acggcggtaa aactccagcc cgagtaacgt
     2941 tggttcgccg ccctgccagg taaaagccac ttcgttttgg gtttcgctgg cagcgatata
     3001 gtgacggaca tacgcttcca gcgtgtcatc gtccatatgc gtgactggct tttcgtggta
     3061 aagggattgt ttttcgagat aaaaacagta gtcacagttc agattacaat cagaaccgct
     3121 cggtttcgcc atcacatgaa aagcacgcgt tggaacctgt tgctgcatgg tcgctccttg
     3181 cctgaagtca tccttcaata agttaagggc gaaatcatac cgtggcgcaa tgcgttatta
     3241 cgggcgttta ttgcgaaggg aaagtgacga aatgcacaga aaacaaaaaa ccccgccgaa
     3301 gcggggttca aaattggtcg gcgagagagg attcgaacct ccgacccact ggtcccaaac
     3361 cagttgcgct accaagctgc gctactcgcc gaaatactgc tttttgaatt tttagttcaa
     3421 ttctttaaag tcgtggtgcg agggggggga cttgaacccc cacgtccgta aggacactaa
     3481 cacctgaagc tagcgcgtct accaattccg ccaccttcgc attgtcacaa cttctaataa
     3541 tggggtggct aatgggattc gaacccacga caactggaat cacaatccag ggctctacca
     3601 actgagctat agccaccact acaaatcttg ttacgcggta ttactaccac cgcagctcaa
     3661 gcgccgggac taaatggcgc gcccgacagg attcgaacct gagacctctg cctccggagg
     3721 gcagcgctct atccagctga gctacgggcg cttagcgccg ttgcgggggt ggataatacg
     3781 gacttcccac ccctctgtct agtccctttt taaataaatt gcgcgtttgg ttacggtttg
     3841 tgcgttttgc tgcttattcc tccagtttat gcgctttacc gtggcgatta agcccaaaaa
     3901 ctttataaat cgccgtcacc gtcagcataa agatgatgcc gacaaacagc gacatacgcg
     3961 tatcttcatt aaagtacatg ccgatcaaaa cgcaaatcag gaatgccatt gttacgtagt
     4021 tggcccaagg gaacaggatt gagcggaacg gatggctggc aattgccgct ttatgcgcac
     4081 gacgaaaacg cagctggcta atcaatatca caaaccatgg caccatcccc ggaagcacgc
     4141 tggcactgta gacatagaca aacacacgct gcggattggg aatgatgtag ttcaggcatg
     4201 agccaattag cagaatagca atagataccg ccacacccgc aaccggtacg ccgtgacggg
     4261 aaactttcgc catcgccgcc ggtaactgac ggtttttcgc cagtgcatag agcatacgtc
     4321 cgcaactgta catgccactg ttacagccag aaagcgctgc cgtcagcacc acaaagttaa
     4381 taatgcccgc cgcagcggta atgccgattt tggcgaaagt cagtacgaac gggctgccgt
     4441 tgctgcctat ttcattccac gggaagatgg taacgataac gaaaatcgcg cctacgtaga
     4501 aaatcaggat tcgccacagc accttgccta cagcgctacg cagcgtcacc tgcggattct
     4561 tcgcttcacc ggcagtaatg ccaatcagct ccacgccctg gtaggacgcc accacaatac
     4621 acaatgcggt caggaaccct ttccagccac ccgcaaagaa accgccatgc tctgtgagat
     4681 tgctaaaacc aatcgactgc ccgccattgc caaagccaaa gaaaatcacg cccaggccaa
     4741 tgacaatcat cacgataatc gtggtgactt tgatcatcgc gaaccagaac tcgatttcgc
     4801 cgtacaacct caccgcagcc agattcgcca acgccaccag cgccaccgcg atcaatgcgg
     4861 gtatccactg cgccatctcc gggaaccaga actggacata aacgccaatg gcggtgattt
     4921 ctgagatccc caccgccatc cacataaacc agtaagacca ggcggtgaga tagccaaaga
     4981 acgggctcat ataacgatgc gcgtaaacgg cgaacgaacc ggtaaccggt tcgaggaaca
     5041 acatttcgcc cattgaacgc atgatgaaaa agacgaacag cccggcgatg atataggcca
     5101 acaatacgga tggcccggcc catttcaggg tactggcggc ccccataaac aggccgacgc
     5161 caatggtgcc ccccagggcg atgagttcga tatgtcgagc ttccagccca cgctgtagct
     5221 ctggtttgtt atctgccata aatcctcgtg ttgtgtttgc atgctttccg gtgttaccgg
     5281 ttatcgttat gggtacatcg agtgttgcga atgttttcgt aattcaggag aaatggcaaa
     5341 taaagcatta aaaatttgaa tgctttgtgt aataaaaaag cagacaggcg acggagtgac
     5401 cacttcgtcg ctttacaaag agaggaaaat cagaggttgc cggtatagtg ccagcgtaaa
     5461 taacgcagca aacgaagctg acgcttaatg cggctcggct gcgaaagcag gcggtagagc
     5521 cactccagcc ccagcgtttg ccagattttc ggtgcgcgtt ttacgtgacc ggtgaaaaca
     5581 tcgtaggtcc cgccaacgcc catatacagc gcatctggat gcaccagacg gcagtcgcgc
     5641 atgaagatct cctgctttgg cgatcccatc gcaacggtga cgatttgcgc tccgctggca
     5701 tgaatgcgtt caaacagcgc ctgacgctgc tcgggtttaa aataaccatc ctggctgcca
     5761 acgatattca cattccactg gttgcgcagt ttagcttcag tttgcgccag cacttcaggt
     5821 ttaccgccca caagaaatac cggtgtccct tctttgcctg cgcgcgccat cagctcttcc
     5881 cagagatcgg ccccagcaac gcgggaaacc tgcgcctgcg ggtacttttt acgtactgaa
     5941 cgtacaacgc ttatgccatc cgcatattta aattcggcag cgttaattag ctcccggacc
     6001 tcggcgttat cttcaatggt cagcattttt tcggcattaa tggcaaccag cgttccttgc
     6061 ttaagctgcc cgtcagcgaa cagataatcg agggcgtgct gcatatcacg ccaaccaatc
     6121 aactgtaagc cacgcagcgt ataggtcggt gccgtggtgt tgttattcat tattatcctt
     6181 caacctgcgt ccggagcgat gattttgttc gtttatgaat gagtccggcg ctttcaaaaa
     6241 gccagtacaa cagttttgcg atcatcagac atgcgccgaa gaccacgata aaaaagacca
     6301 cgcgtgagac aaacgaatcc agcccttcac gcgccagcac gatcatattg aagatcgccc
     6361 cgaagcagaa actgtgcaat atcgcagcct tatagcgatt agtctcgcgg ttgcccagct
     6421 catacagcca gtcgaaccat ttgatgatca gtccaacaac aatcgccccg agcgggatga
     6481 acaacgcgcc gcccatcacc accagtgagc ctataagcgt aggcgagatc gccagtccgg
     6541 agtggttatt cagcacttcc caggtaaagt agttggcgga gttcagcacc atactcgggc
     6601 gacccggcca cagccaggaa gggataaaga catagaaatc gcggacaatt ggagccaggc
     6661 cctggaagtc gatgttgtcg tagttctgca acagcaacgc cagattctcc cacggtgaaa
     6721 aggtgtcgcg agtgagatag agaaacgtat agaacgcttc atcgccgctc acattcattc
     6781 catagcgttt taacgccagc cagaacatgc ccacaatccc cagcacgccc gccgccgcca
     6841 gcatccacaa cgaaatccag ccgcgaataa tgccaataaa caggaagata gcgaatgcga
     6901 tgatgatatt ggcgcgagtg ccgccgacaa tcatataagt cagcaagcca aaagcgaccg
     6961 tgctgacgag gaaaaacagc cacgctttgc tgtcctggcg cagaaagtag accaccagca
     7021 tcgccgggat gaaaaagtaa aagaagcgtt ttaacgccac gccggagact tcactggaga
     7081 agatctgact gtaggagtta agccggaaca gcaaaaagcc gttgtgcatg aagaagatgc
     7141 cgacgcttac cagcgcgatg cccatcagga tcacccacgt gagattggtt tccacgcggt
     7201 tcatggtaaa cagcggacgg cgcggtacat cagcaacgcg tttgcgtagg cgggttttgt
     7261 aggtgacgta gtaaaccgcg tagaagcagc ccgcagaaag caacgcctgc aacaagattt
     7321 ctggcggcgc ggcaccaaca tcaaagcgaa aaaccagcac gctggtcagc gggaagccga
     7381 agaaaaaggt gagcaaaaac agcaatgaaa agaagacatt gaagttaaag cgcacacggc
     7441 gaaactcaaa ccaggtcagc gtggcaataa acagcgtgca gagcagccag acaacaaaca
     7501 ggccactgaa ttgcagcaga ctcatgcgac ctccccggcg gcaatcgcca acgcccgctg
     7561 ccagccttgc agatagttcg ggctaaagaa ggcaatggtg tttttatcca ccgacaccaa
     7621 ctggcgctgc gcttcacgca caatatcctc gttgagatcg tcggtagtaa acagcaccgg
     7681 caaatgctgt tccgtcatat cctgccagaa cggattttcc cggttaagca cacaaggaat
     7741 gcccgcctga atcagtaagc acagcgtacc aatgccctgc tggcgggcaa aaataaagta
     7801 accaagatcg cactgacgaa gtagcgccag ataggcgtca aattccagtt tttcgctcag
     7861 agtttgtaga ttttcttcgc tgaataactc cagccccgcc tgacgaactt cctcgatgta
     7921 cgcttcgtta ttaggcggat atcccatcgg caccaccact tttaccgtat cgccaaattg
     7981 ctgataaacg gcgcgcaagg cagcaatatg ttcattgctg cggtcaccgg agttccccac
     8041 cagaatggtc attttccctt cacgttgccg atcattcgcc atcatattga gtgaagggtc
     8101 catccgcgtc gggaagtaca gcagttctcc ccgcaccttt ggatgcgttt tggcaaaaaa
     8161 gctcaaatca ccgcgggtgg caaatacaca gccgactctc ttttgcgcca ggcgacgtag
     8221 cgggtaaaaa agcttatatc tcaagccact ggaaagctcg tacaggtctg ccccccagat
     8281 atgccagtaa aactggctgg gcttaatgcc accactcagc agagccagcc acagtgtggg
     8341 attgaactga ccgtggaaga aaaaacgctg ctgacggtta gcttttgctt tcgcgatgac
     8401 cgcttccgcc agcgattttt tcccagggaa aaattgcaca gaaagcgccg gacagctatc
     8461 acttaagccg tcgtccttgc caacaaccat aaactcgcgc gcgtgctcgc tcgtcgcggc
     8521 cagcgcgtca ttgaaaaacc gcaaaacggt tcggttatgg tgagggatat ccgatcccag
     8581 tacgtgaatc agtacagtca tgcccgccta cgccagagta aaaacacgcc acaacaaaga
     8641 gaaaaataga cgatataggt tgccatatag gcctgcgccg cgcccagtgc accatgcgca
     8701 gggattagcc agtgggcaaa taccatcaat aaagtgaact ggctgacttc cgccagaata
     8761 taaaaccgca gtgacgcttt ggcgatcacc agataaccaa agacataagc gcccactttt
     8821 aacacatcac ccaccaactg ccaggcaaag agatcgcgca tagcggtaaa tttattcgac
     8881 aacagcagcc agatagcaaa atcacgcagc aaccagacgg taaaactcgc cgccgccact
     8941 gccggcaaga cgaatttaag cgatttaacc acttcccggg tgatatcgcg cttttccgtt
     9001 agccgcgaca acgtgggcag caaatatacg ctgaacgatg ccgtaataaa ttgcaggtag
     9061 gcatcggaaa tactgctcac cccttgccag atccccacct catcccagct atactgcgcc
     9121 gccagcagtt tacgcatcat gatgtaagca acaggcaagg tcaccgacgt aatcaacgcc
     9181 atgagcgtaa atttgctcaa ctgccctgcc agaccgttat cccagctggg tttcagatag
     9241 cttaacggga tgacaccacg tttgatcaac atgatggcgg caggaattac caccagcgcg
     9301 ggaatcagcg ccagacccag caacgcccct tcataaccgc ccaaacggta actgacgtag
     9361 tacgcgagaa cgccaatcaa gctgccaaca atcagagata acgcattacc tgcggcatcg
     9421 cgaaagcctt tcatcagcgc cagtaacagg ttgccccagg cgatccccat ttgcaccagc
     9481 gccactaaac gcaccagccc ctgatagtcg gtattaccaa acaatccctg gctgattggc
     9541 gcagctgcca gcacaaaaac cagcgccatc agcgtagaga aaccaagtac catcgctgat
     9601 gaagtgccga ccacgcggcg cagctgttgc ggattatcat ggtactgggc aacgtatttg
     9661 gttacaccgt taaagatgcc agccccggca agcacgccga gcacggtaat caactggcgg
     9721 aaatttgccg ccagcccaag ccccgccgga ccaaatgaca ccgccagcaa cttaccgact
     9781 agtaacccgg caccaatctt gaccagtgta ctggccgccg tccacaagga cgcttttgcc
     9841 aacgacatat caggagaagt agttcaacaa agtcgcaatt accgtacgct gattgacggg
     9901 cgacaggttg tagaacagcg gcaggcgcag caggcgctcg ctctctttgg tggtgtagcg
     9961 atcttcaccg tggaactcac caaagtgttc ccccgcaggg caaccgtgca gcggaatgta
    10021 atgaaacacc gccatgattt ccgcttcttt cagaaagtta atcaacgcgc tccggtcatc
    10081 aatatcccgc agtttaatgt agaacatatg cgcgttctgc acgcagccat cgggaatcga
    10141 cggcagctcg atacgcccat tttttgccag aggcgccagt gcatcgtagt agttttgcca
    10201 cagcgccaga cgctgctgat tgatacgatc cgctgcttcc agttgcgccc acaggtacgc
    10261 tgcttgcaga tcggacatca aatagctgga gccaatatcg cgccaggtat atttatcgac
    10321 ctgaccacgg aagaactggc tgcggtttgt gcctttttca cggatgatct cggctcgttc
    10381 gatcaacgct ttatcgttaa tcagcgtcgc gccgccttca ccacccgccg tgtagttttt
    10441 ggtttcatgg aagctaaagc agccaatatg accaatggtt cccagcgcac gccctttgta
    10501 agtggacatc acgccctgag cggcatcttc caccacaaat agattatgct tcttcgccaa
    10561 cgccataata gtgtccattt cgcaggccac acccgcgtaa tggaccggca cgataacgcg
    10621 cgttttgtcg gtgatcgccg cttcaatcag cgtttcgttg atgttcatgg tgtccgggcg
    10681 aacatccaca aagacgattt tcgcgccacg cagcacaaag gcattggcgg tggagacaaa
    10741 ggtgtagctc ggcatgatca cttcatcgcc aggctggata tcgagcagca gcgccgccat
    10801 ctccagcgaa gcggtgcagg acggcgtcag taacactttg gcgctgccaa aacgttgctc
    10861 cagccactgc tggcagcgac gggtaaaacc accatcgccg cacagtttgc cgctacccat
    10921 tgccgactgc atatagtcga gttcggttcc caccaccggc ggtgcgttaa atggaatcat
    10981 gtgatcacct gtataaccag tacgcggtgc tttctacatt cgcaccactt tgtatgtatc
    11041 gtttaagcgc ggcggtgttg cccatctggg tcgccacccg caaagttgtt ttaccgcgag
    11101 catacgccca gtttagcgcc gtttgcatta gctcagcacc tgcaccgcgt ccagccagca
    11161 ggccaattcg cgcatctgtc gcattgagtt cccgtaaaga gacataaccg cgaatatcgc
    11221 cggacgccgc acgtaaaatc agacattgat gatcaaaggt gccgcgcacg gcattttcaa
    11281 tccactgtgc ataaaagcga ccgctggcgt caggcgcata ccacggcgca cgaaaacggc
    11341 tttgcgcaaa tgcggcgctg gctaactgac gtaatgcggg aatatcggtc tcttgtgcca
    11401 ctactgcacc gctatcactg acattgttca cgggtagtgc caaatcaact tcaccttcta
    11461 tcagggagaa tcccagctgt tgcagagcat ccagttcacc cgtatttgat gccgcaattt
    11521 tggcctgcac ccgtgaccac ggcgctaagg cgtctggcgt caggagtgcc gcgtcagggc
    11581 taatacgcac aatggcgctg ttaacaccaa agaaggcgtt ttcccaggtt agtggctcaa
    11641 tactggcgcg gacgggcacg aagtaactcc agcagatatt ggccgtagcc agttttcgct
    11701 aatgaactgg cagcacgctt cacaccctcg tcatcgagcc agccgttacg ccaggcaatc
    11761 tcttccaggc aggcaatctt aaagccctgg cgtttttcta ctgtctgcac aaaagtgctg
    11821 gcctcaatca ggctgtcgtg agtgccggta tccagccagg caaatccgcg cccgagcagt
    11881 tcaacggtca ggttgcccgc ctcgaggtac atctggttga tggaggtaat ctccagttca
    11941 ccacgctccg acggcttcac ctgctttgcg tactccacga ctttactgtc gtagaaataa
    12001 agcccggtca ccgcccagtt tgatttcggc tgttttggtt tttcttccag cgagatagcg
    12061 cggaaattgt cgtcaaactc caccacgcca aagcgttccg ggtccatcac ctgatagcca
    12121 aataccgtcg ccccttccgt gcgcgccgca acatgacgca gtttcggact gaagccctgt
    12181 ccgaagaaga tgttatcgcc cagcaccaga caagaaggtt caccgttgag gaaggtttca
    12241 ccaatgataa aggcctgcgc caggccgtcc ggactgggct gttcggcata ttccagctgg
    12301 ataccgaact cactgccgtc acccagcagg cgctggaaat aacctttatc ttccggcgta
    12361 gtgatgatga gaatttcgcg gataccggcc agcatcagca ccgacagcgg atagtaaatc
    12421 attggcttat cgtaaatcgg caacagttgc ttagatacgc cgcgcgtaat cggatgcaat
    12481 cgggtgccgg aaccgcccgc caggataata cctttcatgt ttgcctccgc tgaaaattag
    12541 cctttcagac ctaaacgctc gccctgatag ctgccgtcct gcacctgctt ccaccagctt
    12601 tcattagcca gataccactg caccgtttta cgcattccac tttcaaaggt ttcctgcggc
    12661 agccagccaa gttcacgggc aattttcgaa gcatcaatgg cataacgcag atcatgcccc
    12721 ggacggtcag cgacaaaggt gatcaggtca cgatagtgcg ccacgccgtg cggcttattc
    12781 ggagccagtt cttccagcag ctcgcaaatg gtttccacaa catcgagatt cttacgctcg
    12841 ttgtgaccac caatattata ggtttcaccg actttcccgg tggtcgccac gcaatacagc
    12901 gcgcgggcgt gatcttcaac atacagccag tcacggattt gctgcccgtt gccatatacc
    12961 ggcagcggtt tacccgccag cgcgttgagg atcatcagcg ggatcagttt ttccggaaag
    13021 tggtaaggac cgtagttatt cgagcagttg gtgataagcg tcggcagacc ataggtccgc
    13081 agccaggcgc gcaccagatg gtcgctgctg gcttttgacg cggaataagg gctgctcggc
    13141 gcatacggcg tggtttcggt gaagaaatca tccgtcgagt gcaggtcacc atatacttcg
    13201 tcggtggaga tatgatgaaa acggaacgct gattttttat cttccgtcag cgcagtccag
    13261 taagcccgcg ccgcttcaag cagtgtataa gtcccgacaa tgttggtttc aataaacgct
    13321 gccgggccgt caatagaacg gtcaacatgg ctttcggctg ccagatgcat gacacagtct
    13381 ggctgatact cagtgaatac gcgcgccagt tctgcccgat cgcagatatc aactttctca
    13441 aaggcaaagc gctcgctttg cgcgaccggt gccagcgaca tcaggtttcc ggcgtaggtc
    13501 agcgtatcga ccaccaccac cgcgtcgctc gtttcgttga tgatataacg caccagcgcc
    13561 gagccaataa acccggcacc acctgttatc agaatctttc tcatcagcgc cagactcctt
    13621 tggcatcgac gacatactgc tgatggacgt tgtcgccatt gataactttg aactgactat
    13681 gatcgaccag catcaccagc acatctgccg ttgccagcgc ctcgtcaagc tgcgccagag
    13741 tacaaagccc ggtcagtttc ttcggcaact ggtggatgtt aggctcaaca accagcgttt
    13801 cgccgctgtg ccactgggcg atcagttcag cgatttccat tgccggactt tcgcgcaggt
    13861 catcaatatt cggtttaaac gccagaccaa agcaggcgat tttcagttca ttggcgcgtt
    13921 tatcggtagc agccagacaa tcagccaccg ccgctttcac ctgatcgata acccagaacg
    13981 gtttgtgatc gttcacttcg cgcgcggtac ggataagccg cgcctgctgg gggttctgtg
    14041 ccacgataaa ccacggatca acagcgatgc agtgaccacc cacgccaggg cctggctgaa
    14101 gaatattgac gcgagggtga cgattcgcca ggcgaatcag ttcccagacg ttaatcccct
    14161 gatcggcaca aatcagcgac aattcattag caaaagcgat attcacatcg cggaagctgt
    14221 tttcggtgag cttacacatt tccgccgtcc gcgagttagt gacgacacac tcaccttcga
    14281 ggaaaatttt gtacagttcg ctggcgcggg ccgaacaaac cggcgtcata ccaccaatca
    14341 cgcgatcgtt tttaatcagc tcgaccataa cctggcccgg taatacgcgt tccgggcagt
    14401 aagcaatgtt gacgtccgcc aactcgccca cctgctgtgg gaaagtgaga tctggacgca
    14461 tctctgctaa ccattctgcc atcttctcgg ttgaccccac cggcgaggtg gattcaagga
    14521 tcactaacgc gccttttttc agcactggcg caatggagcg agcagccgat tcaacgtagg
    14581 tcatatctgg ctcatgatcg cctttaaacg gtgtgggtac agcaatcaac caggcatccg
    14641 cttcaactgg cgtcgtgctc gctcgtaaaa aaccgccttc tacggcagtt tttactacgc
    14701 tcgccaaatc aggctcgacg atatggattt cgccacgatt gatggtatca accgcgtgtt
    14761 ggttgatatc gacaccaatc acctgttttt gccttgaggc aaacgcagct gctgtaggca
    14821 gcccgatgta acccagtccg ataacagaaa tggtcgcaaa actcatagtg atatccgatt
    14881 attttttaac gcttccagaa tgcgagagca tgcctgacca tcaccatacg ggttatgggc
    14941 gcggctcata gcttgatatt cgttttcgtc ttttaaaaga cgcgtcactt cctcgacaat
    15001 tcgctgctta tccgtgccga ccagacgcac cgtacccgcc gtcaccgctt ccggacgctc
    15061 agtggtatcg cgcatcacca gcaccggttt ccccagcgaa ggcgcttctt cctgaatgcc
    15121 gcctgagtcg gtcaaaatca gccaggcgtg gttcatcagc cagacaaacg gtaaatactc
    15181 ctggggatcg atcagaatga catttttcac atgccccaga atgcgattga ccggctctct
    15241 gacgttcggg ttgagatgca ccggatagac aatctggatg tcctggtgcg tggtggcgat
    15301 gtctgccagc gcatggcaga tttcttcaaa gccacgaccg aagctctcac gcctgtgacc
    15361 ggtcaccaga atcatctttt tatcggggtc gataaacggg taatttgccg ccagttctga
    15421 acgcagcgta tcgctgctca tcacctggtc acgcacccat aacagtgcat caatgactgt
    15481 attaccagta atgaagattc ggctatccgc aacgttttca cgcagcaagt tttgccggga
    15541 agtttcggtt ggagaaaaat gatacatcgc cagatgcccg gtcaatgtac ggttagcctc
    15601 ttccggccac ggcgaataga gatcgcccgt gcgcagacca gcctcaacgt gaccaacagg
    15661 aatacgctga taaaacgccg ccaggctggt tgccagcgtc gtcgtagtat cgccgtgaac
    15721 cagcacgaca tctggtttga actcggcaag aataggtttt agcccttcca gaatccgaca
    15781 ggttatctct gtaaggcctt gtcctggctg cattatgttg agatcgtagt caggtacaat
    15841 ggaaaagagt ttcagcacct gatcgagcat ctcccgatgc tgcgcagtga cgcaaacttt
    15901 agcctcaaaa aaaggatctt ttgccaacgc atgcaccaac ggtgccatct tgatggcttc
    15961 cgggcgcgta ccaaatacag tcagtacttt cacatcgatt ctcttcgaat aagcggcgag
    16021 cgcctttgcg ctcaccgcag cagtgttgct atttcgagca acggcgggtt aatgcgacac
    16081 cagccccgat cagtcccccg acaatgcccc acataatcat caggaaggca cgacgtgggc
    16141 tatcgcgttt taccggttct tccggcgtac gcaaatagcg ataggtctga aaacgcggat
    16201 ccagggttgg accaacattc agggtgttta acatggcccg attttgatca tagtcgagat
    16261 caaaggccgg accgacggcc tgtaaatttt ccagtcgagc ctggagcatt ggacgcccaa
    16321 gcaggaacat ttctgaatca ggtaattcct cggcaggcac atctgtcgca ctgcgcgaaa
    16381 tattatgctg ctcagcaatt ttcagcgcct gttcaatact gttcatccgg cggtcataga
    16441 tggctttcgc cacctcttcc tgacgcttca cctgagcttt catctggata gtgcgtgccg
    16501 cccatgcgcc tttcagctca tcattcagat ggctggctgc acgctggctg gcaaaagcaa
    16561 catactgacg taacaggtta ttagcgtcag gcgcggtttc ggcaataagc ttcacgctgt
    16621 cattgaccgc gcgggtaaag tcaccgggaa taaacacgat gttgttaatc atttcatcca
    16681 gcaacgccgc atcggctttg ctgttgccca ccatccgctg tttgtaatag tcggtttgca
    16741 gccagaactc tctgcgggta tcccacgagg ccagttgcat aacaaactct ttataagctt
    16801 cgtccatgac cgatggttgg tcggcagaag ccatgtttga acggacatcc aggttacgca
    16861 aaaattgctg ctgtgagtag tacccgccca gcatattcac cgttggacga tcggtaatcg
    16921 ccgtcgcgct ccactcctga cgagcaaaaa aagtatacgc cagcgcgatt aacgcaaacg
    16981 ccagccccat gccaataatc catagcttcc cagcccacaa ggtacgaaac aacccacgaa
    17041 tatccagttc attttcagcg tcttcggccg gtttcccagg cattggttgt gtcatcacat
    17101 cctcatttat ttggttaaat tggggctgcc accacgattt ctacgcagtc tgcgttttac
    17161 gcgcttaata aagcgagcaa ctttccaggc acgcttaatg caatatccat agaggaagaa
    17221 tgctagcaaa aagagcacca gcatgaccca ctccgggaca aaatgagaat attctgccag
    17281 cacgccaatg gaagcgagca gtgctgcggc aagggtaatc agcacaaacg cctggcggga
    17341 agtaaacccg gcacgcatga tcaaatggtg aatatgctga cggtctggag agaatgggct
    17401 catgccttta cgcaggcgac ggtacataat cgccaccata tccattagcg gaatggcgat
    17461 aatccacaaa gcggtaaccg ggctgatggg atgagttttg ccctgggtcg tttcgagcag
    17521 gatccagatc acggtaaaac caatcagcgt actgcccgca tcccccataa agactttgta
    17581 gcggcgaccc aggataccaa ggttaagcat gatgtatggc aggatggcgg cgatcatcgc
    17641 aaagcaccag attgcgagac tggtttgccc gtcgaaccac aaaatcatac cgattgctgc
    17701 aaacgagacg caggacaacc cgcccagcaa gccatcaatg ccatcaacca tgttgaacgc
    17761 attaatggcc gcccagacgg caaatagcgt caggaagtaa ccaaacggtc cgagcaccat
    17821 ctcccaggag ccaaagatat aacccaggct actgagataa agtttgccga acaccatcat
    17881 aacaatgcca acagcggcct gtatggtggc tcggattttt acgctaatat caaaacggtc
    17941 atccagcgcg ccaatgaaaa caagcacacc agcacaagcg agatagagag atgcctgcgg
    18001 aatatagtag tcgacaattc cgaacgtgaa gcaaatccct gcgtaaaccg aaatcccccc
    18061 aacgagaggt atcaatccct ggtgacgttt gcggaagttt ggtttatcca ctaaaccgac
    18121 tttttttgcc accttacggg caaaaaacag aaacagtgtc gtgaataaaa aaatactgat
    18181 gagatcagta ctcactgtca gtaaattcac aatgcatgct ctcagagaaa attattagca
    18241 gaagtataac cacgaagacc tttattcaga aaggaaagtc cgttccgaac ctaccagcac
    18301 tatgcgaaaa ttaaccactt gatatgcaaa ttgcataaca caccagcaaa ttccaagaat
    18361 tttcctaaca tcgccagcgc ggcattaaga ttacagatat aaaagcaaaa cgccacgtaa
    18421 acacgtggcg tttttggcat aagacaaatt tatgagcgtt tcatcatttc gaagaaatcg
    18481 tcattggtct tggtcattgc cagtttatta atgaggaatt ccattgcatc gatttcgccc
    18541 atcgggtgaa tgattttgcg caggatccac attttctgca gttcttcctg agtcgtgagc
    18601 agctcttctt tacgggtacc ggaacggttg tagtcgatag ccgggaagac gcgtttttca
    18661 gcgatcttac gagagaggtg cagttccatg ttgcctgtac ctttaaactc ttcgtagata
    18721 acttcgtcca ttttagaacc ggtatcgata agcgccgtcg cgataatggt caggctgccg
    18781 ccctcttcca cgttacgcgc cgcaccgaaa aagcgtttcg gacgatgcag ggcgttggcg
    18841 tccacaccac cggtcaacac tttacctgac gccggaacaa cggtgttgta agcgcgcgcc
    18901 agacgagtga tggagtcgag cagaatgata acgtctttct tgtgctcaac caggcgtttc
    18961 gccttctcga tcaccatttc cgcaacctga acgtggcgag atgcgggttc gtcaaaggta
    19021 gaagcaacaa cttcaccttt taccagtcgc tgcatctcgg ttacttcttc cggacgttcg
    19081 tcgatcagca gaaccatcag cacacaatcc gggtggttgt aagcaatgct ctgagcaatg
    19141 ttctgcagca gcatggtttt accggctttc ggcggtgcca caatcagacc acgctgacca
    19201 cgaccgatag gtgatgccag atccagtacg cgagcggtta aatcttccgt agaaccgtta
    19261 ccacgttcca tacgcagacg agagtttgcg tgcagcgggg ttaagttctc aaagaggatt
    19321 ttgttgcggg cgttttcagg tttgtcgaag ttaacttcgt taactttcag cagcgcaaaa
    19381 tagcgttcac cttctttcgg cgggcgaatc ttaccagaga tggtatcacc agtgcggagg
    19441 ttgaaacggc ggatttggct aggggaaacg tagatgtcat caggaccggc gaggtaggag
    19501 ctgtctgcgg aacggaggaa accaaatcca tcctgcaata tctccagtac gccatcacca
    19561 aagatatctt cgccactctt tgcgtgctgc ttcaggatgg caaaaataat gtcctgctta
    19621 cgcatacgag ccaggttttc cagccccata ttttcgccga gagtgatcag ctcagaaacc
    19681 ggcgtattct ttaattcggt aagattcata gtggtgtgag ttcttaaact tggggtaaat
    19741 ctcgaactta atgttgtgaa tggtatggca ggatcatcca tgcctgttta cggtcatcga
    19801 ctcatgtctt ttcgctgcct ggtcacagga gagtacgcag aactgaaacg acaagacgga
    19861 ttgagtgacg agccagaaat ctgttcactt cgcatttaag ataaaacggg aagcgttgga
    19921 aattacaaga ttcaaactta ataaggtatg tttaatacga agtcaacact aagttagcat
    19981 gactcacgcc gggcgtccag tttttagcga cggggcaccc gaacatgaaa ttcccttacg
    20041 ccaggttagc gtcgaggaac tctttcaact gacctttaga cagtgcgccc actttggttg
    20101 ccgccacttc accgtttttg aacagcagca gagtcgggat accacggatg ccatatttcg
    20161 gcgcagtgcc agggttttga tcgatgttca gttttgcaac ggtcagtttg ccctgatatt
    20221 cgtcagcgat ttcatccaga atcggggcga tcattttgca cggaccgcac cactctgccc
    20281 agaaatcgac gaggatcgcc ccgtccgctt tgagtacatc cgtgtcaaaa ctgtcgtcag
    20341 tcaggtgaat aattttatcg ctcatatata actccacagg aataagcctg gcgtgttggt
    20401 ttcgttgttg gtgtaacatt aaccaactaa aggttgactt tatttcaccg gatacgcttt
    20461 cgtaaagcaa tagtaagctg atattctacc acactatgag caaaacacat ttaacagaac
    20521 agaagttttc cgacttcgcc ctgcatccga aggttgtaga agcccttgaa aaaaaagggt
    20581 ttcataactg tacgcccatt caggcactgg cccttccgct gacgctggcg ggtcgtgacg
    20641 tagccgggca ggcgcaaacc ggtaccggga aaacgatggc gtttcttacg tcaacgtttc
    20701 attatcttct ctctcatccc gcgattgctg atcgcaaggt gaatcagccg cgtgccttaa
    20761 ttatggcacc gacgcgtgaa cttgccgtgc agatccacgc cgacgcggaa ccgctggcgg
    20821 aagctaccgg cctgaagctg ggtctggctt acggtggtga tggctacgac aaacagctga
    20881 aagtgctgga aagcggcgtt gacattctga ttggcaccac gggtcgttta attgactacg
    20941 ccaagcagaa ccacattaac ctcggtgcca ttcaggtggt ggtgctggac gaagccgatc
    21001 gcatgtacga tctgggcttt attaaagata tccgctggct gttccgccgt atgccgcctg
    21061 caaaccagcg cctcaacatg ctgttctccg ccacgctttc gtaccgggta cgtgaactgg
    21121 cgttcgagca gatgaacaat gccgaatata ttgaagtgga accggaacag aaaacgggtc
    21181 accgtattaa agaagagctt ttctaccctt ctaacgaaga aaaaatgcgt ttgctgcaaa
    21241 cgctgatcga agaagagtgg ccagacagag cgattatttt cgccaacacc aaacaccgtt
    21301 gtgaagagat ctggggccac ctggcggcag atggtcatcg tgtcggttta ttgacaggcg
    21361 atgtcgcgca gaaaaaacgt ctgcgtattc ttgatgaatt tacccgtggc gatctggata
    21421 ttctggttgc caccgacgtt gccgcgcgtg gtttgcatat tcctgctgtg acacacgtct
    21481 ttaactacga tttacccgat gactgtgaag attacgttca ccgtattggt cgtacaggtc
    21541 gcgctggcgc aagcggtcat tccatcagcc tggcgtgtga agagtatgca ttgaatttgc
    21601 ctgctattga gacctatatt ggtcactcaa ttccggtaag taaatacaat ccggacgcat
    21661 tgatgaccga tctgccaaaa ccgctgcgcc tcacgcgccc gcgcacaggc aatggtccgc
    21721 gtcgtactgg cgctccgcgt aatcgtcgtc gttcaggtta aagaaaaaat gtcatactcc
    21781 aaatcattca agctgtatca agatggcaag agaatgaatc ccgatgagtt tactgtcgta
    21841 agtgattcag gtgaaagaat gcagccaaca cagagacaga ttgaaggatg aagagtatgg
    21901 gttccacctc gtcgctgtat gcagccattg atctcggttc gaatagtttt catatgctgg
    21961 ttgtgcgcga ggtggctgga agcatccaga cgctgacgcg aataaaacgc aaagtgcgtc
    22021 tggctgctgg cctgaatagc gaaaatgccc tgtctaatga agcaatggag cgcggttggc
    22081 aatgtctgcg cctgtttgct gaacgtctgc aagatatccc tccctcgcaa attcgcgttg
    22141 tcgctacggc gacattacgc cttgccgtca atgcgggtga ttttattgcc aaagcgcagg
    22201 aaatcctcgg ttgtccggta caggtgatca gcggtgaaga ggaagcgcgt ctgatttatc
    22261 agggcgttgc tcacaccact ggcggtgccg atcagcgcct ggtggtggat atcggcggag
    22321 ccagtactga actggtaacc ggcacgggtg cacaaaccac ctctttgttc agcctgtcga
    22381 tgggctgcgt tacctggctg gaacgctatt ttgccgatcg taatctgggg caggaaaatt
    22441 ttgatgctgc agaaaaagcg gcacgcgaag tgttacgtcc ggttgccgat gaattacggt
    22501 atcacggctg gaaagtgtgc gttggcgctt ccggcaccgt gcaggcgtta caggaaatca
    22561 tgatggcaca ggggatggat gaacgcatta ccctggaaaa gttgcagcaa ctgaaacaac
    22621 gcgccattca ttgcggtcgg ctggaagagc tagagattga cgggctgacg ctggaacgtg
    22681 cgttagtgtt cccgagtggt ctggcgatcc tgatagccat ttttaccgaa ctgaatattc
    22741 agtgtatgac cctggcgggc ggtgcgctgc gtgaaggcct ggtctacggt atgttgcatc
    22801 ttaccgtcga gcaggatatt cgcagccgta cgctgcgtaa tattcagcgc cgctttatga
    22861 tcgatattga tcaggcacag cgcgtaacca aagttgcggc taacttcttc gatcaggtgg
    22921 aaaatgaatg gcatcttgaa gcaataagcc gcgatttgct catcagcgcc tgtcagcttc
    22981 atgaaatcgg cctgagcgtt gacttcaaac aagcgccgca acacgctgct tatctggtgc
    23041 gtaacctgga tcttcccggt tttacccccg cacagaaaaa actgctggcg acgctactgc
    23101 tcaaccagac taatccggtc gatctctcat cgctgcatca gcaaaatgcc gtaccaccgc
    23161 gcgtcgcaga acaactctgc cgtttactcc gcctggctat catttttgcc agccgtcgcc
    23221 gtgacgatct cgtgccagag atgacattac aggctaacca tgaactgttg accttgacgc
    23281 ttccgcaagg ttggctaacc caacatccgc tgggtaaaga gattattgtt caggaaagcc
    23341 agtggcagag ctatgtccac tggccgctgg aagtgcatta atgagtaagt gccggatgcg
    23401 atgctgacac attttttccg gccttgatta tttccctcgt tttgccgcca tcatcgcttt
    23461 cagattcgcc agatggcttt gccctttctg catccgttct tcggcgctga ccactttgcg
    23521 ctcctgttcc caaatcaaat catcctgcgg cagctccagc aggaagcggc tcggctccgg
    23581 gcgcaccagt tcgccgtact gacggcgttc tttacacagc gtaaaggtca attccttctg
    23641 ggcgcgggta atgccgacat aggccagccg ccgctcctca tcaatattgt cttcatcgat
    23701 gctgctctgg tggggcaaaa acccttcttc cataccaacc atgtagacat agggaaactc
    23761 cagccctttc gacgcgtgca gagtcatcag ttgcacctga tccagttctt cctcgctctc
    23821 gccgcgctcc atcatgtcgc gcagagtaaa gcgcgtcacc acctgggtga gcgtcatcgg
    23881 ctcatccagt tcgctgcctt ccagcatctc cgtcatccag ctaaacagtt ggttgacgtt
    23941 cttcatgcgc atttcggcag ctttcgggct gggcgatgtt tcgtacagcc aggattcata
    24001 atccatgccg tggatcagat cgcgcaccgc ggcaatcggc tcccgctccg ccagacgctg
    24061 gatttctgcc agccagtgag tgaagcgggt caatgcttca taaccacgtc cgctaagcgt
    24121 ctgactcagg cccatatcaa agctggcggt aaacatgctt ttattgcgcg tcatcgccca
    24181 ttcacctagc tttttcagcg tcgccgggcc aatctctcgc ttcggcgtgt taacgatacg
    24241 cagaaatgcg ctgtcatcgt ccgggttagt cagcacgcgc agataagcca gcaagtcctt
    24301 gatttcagga cgagagaaaa acgacgtacc accagatatt ttgtacggga tgcggttttg
    24361 catcaggaat ttttcaaata cccgcgactg atggttgccg cgataaagaa tggcgtaatc
    24421 tttgtactgc gttttattga cgaagtgatg ggcgatcagc tcgccggtga cgcgctcggc
    24481 ttcatgttcc tcgttattcg cgcttaaaac ttttagctct gtgccgtaac ccagttcgga
    24541 gaacagacgc ttttcaaaga cgtgcgggtt attggcgatc aggatgttcg ccgctttcaa
    24601 aatacgtccg gaagagcggt agttctgctc aagcttaatc accttcagcg ccggaaaatc
    24661 ctggctcagc agcaccagat tctgcggacg tgcgccgcgc caggagtaga tcgactggtc
    24721 atcgtcaccc accacggtaa agcgcgcgcg gctgcccacc agcagtttca ccagttcata
    24781 ctggctggtg ttggtgtcct gatactcatc caccagcagg tagcgaattt tgttctgcca
    24841 gcgctcgcgg acttcttcat tgcgttgcag cagcaacgtc ggcaataaaa tcagatcatc
    24901 gaagtcgaga acgttacagg ctttcaggtg tgcatcatac agcccataac aatgggcaaa
    24961 aatacggtcc cgctcgccct ttgcctctgc cgccgcctgc gccggtgttt tgagatcgtt
    25021 cttccagttg gagatggtcg aaattagctg ttgcaggaga actttgtcat cttcaatcag
    25081 cccctcggtc aactctttaa gcaaagcaag ctgatcggta tcgtcaaaca gcgaaaagtt
    25141 agccttcatc cccagcgccg catactcgcg tttgatgata tccagcccca gcgtgtggaa
    25201 ggtggagatc atcagcccac gcgcctcttt gcgccccagc gtctgcccga cacgctcttt
    25261 catctcgcgt gccgccttat tggtaaaggt cacagccgcg atatgtcgcg cctgataacc
    25321 gcaactgcgg atcagatggg cgattttatt ggtgataaca cgagttttac cggaacccgc
    25381 gcccgccagc accaggcagg ggccggtaac gaattcgaca gcttgttgtt ggccggggtt
    25441 tagacgcata ggaaaatcgc tcaatgaaag tcgggagggg aaaataacag cgtggtagta
    25501 tagccagcct catccatacc actcaaggca cgatcatggc aaaaacagca gcagcactgc
    25561 atatccttgt aaaagaagag aaactggctc tggatcttct cgagcagatc aagaacggtg
    25621 ccgatttcgg caagctggcg aagaaacact ccatttgccc gtcaggcaaa cgcggcggtg
    25681 atttgggtga attccgccag ggtcaaatgg ttccggcgtt cgataaagtg gttttctctt
    25741 gtccggtact ggagccaacc ggcccgctgc atacccagtt cggttaccac atcattaagg
    25801 tgctgtaccg taattaacag caaggccttc tccgggagaa ggccttgaac tctgaatcgc
    25861 tattaacccg caacagcaat gcgtttcata tctgtcatat agccgcgcag tttcttacct
    25921 acctgctcaa tcgcatggct gcgaatcgct tcatttacat cacgcagctg cgcgttatct
    25981 accgcacctt ccggaatagc tttacccagg tcgcccggtt gcagctctgc cataaacggt
    26041 ttcagcagtg gcacacaagc gtaagagaac agatagttac cgtactcggc agtatcggag
    26101 ataaccacgt tcatttcgta cagacgctta cgggcgatgg tgttggcaat cagcggcagt
    26161 tcgtgcagtg attcatagta agcagattct tcgatgatgc cagaatccac catggtttca
    26221 aacgccagtt caacgcctgc ttttaccatc gcgatcatca gtacgccttt atcgaagtac
    26281 tcctgttcgc cgattttgcc ttcatactgc ggcgcggttt cgaacgcggt tttgccggtc
    26341 tcttcacgcc aggtcagcag tttcttatcg tcgttggccc agtcagccat catgccggag
    26401 gagaattcgc cggagatgat gtcgtccata tgtttctgga acagcggcgc catgatctct
    26461 ttcagttgct cagagagcgc gtaagcacgc agtttcgccg ggttagaaag acggtccatc
    26521 atcagggtga tgccgccctg tttcagcgct tcggtgatgg tttcccaacc gaactgaatc
    26581 agtttttctg cgtatgccgg atcggtgcct tcttccacca gcttgtcgaa gcacagcaga
    26641 gaaccagcct gcaacatacc gcacaggatg gtttgctcgc ccatcaggtc agatttcact
    26701 tccgcaacga aagaggattc cagaacgccc gcacggtgac caccggttgc tgccgcccat
    26761 gctttagcga tcgccatgcc ttcgcctttc ggatcgtttt ccgggtgaac ggcaatcagc
    26821 gtcggtacgc cgaatccacg tttgtactct tcacgtactt cggtgccagg gcatttcggc
    26881 gcaaccatta cgacggtgat gtctttacgg atctgctcac ccacttctac gatattgaaa
    26941 ccatgagagt agcccagcgc cgcgccgtct ttcatcagcg gctgaacgga acgcactacg
    27001 tcagagtgct gcttgtctgg cgtcaggtta accaccagat ccgcctgcgg gatcagttct
    27061 tcgtaagtgc caactttgaa gccgttttcg gttgctttac gccaggatgc gcgcttctcg
    27121 gcaatcgctt ctttacgcag agcgtaggag atatcgagac cagaatcacg catgttcagg
    27181 ccctggttca gaccctgtgc accacagccg acgatgacta cttttttacc ctgaaggtag
    27241 ctcgcgccat cggcgaattc atcgcgcccc ataaagcgac atttgcccag ctgtgccaac
    27301 tgctggcgca gattcagtgt attgaagtag ttagccatgg tgattcctcg tgatgttgtg
    27361 cttcttattg ttcggttcgc gtttgcgaga ttgaattcac tatatgacag gaaatttatt
    27421 gcggaaattg atatattcac aacgtcatat tgcaatttct gcaacgtcaa cattaagggc
    27481 tgcgccattg gatttacgcg atctgaaaac cttcctgcat ctggcggaaa gccgccattt
    27541 tggccgcagc gcgcgggcaa tgcacgttag cccgtccacg ctctctcggc agattcagcg
    27601 cctggaagac gatctcggtc agccgctgtt tgtgcgcgat aaccgcacgg taacgctaac
    27661 cgaagcaggc gaagaactgc gcgttttcgc ccagcaaacg ctgttgcagt atcaacagct
    27721 gcgccacacc atcgatcagc aaggaccgtc gctctctggc gaattacata tcttctgctc
    27781 ggtgaccgct gcctacagcc atctgccgcc gattctcgat cgcttccgcg cggaacaccc
    27841 gtcggtggag attaaactca cgactggtga tgcggcagat gcgatggaaa aggtcgtcac
    27901 tggcgaagcg gatctggcga ttgccgggaa accggagact ctgcccggcg cagtggcgtt
    27961 ttcgatgctg gagaatctgg cagtagtgct gattgccccc gcgctaccct gcccggtgcg
    28021 taaccaagtt tcggcagaga agccggactg gtctacagtg ccgtttatca tggccgatca
    28081 gggaccagta cgtcggcgca ttgagttgtg gtttcgacgc aacaaaatca gcaacccgat
    28141 gatttacgcc acggtcggcg ggcatgaagc gatggtgtcg atggtggcgc tcggctgtgg
    28201 cgtagccttg ttgccggaag tggtgctgga aaacagcccg gaaccggtgc gtaaccgcgt
    28261 gatgatttta gagcgcagtg atgagaaaac gccgtttgaa ctgggcgtgt gtgtgcagaa
    28321 aaagcggcta catgagccgc tgattgaggc attctggaag attttgccga accattaacc
    28381 cgccaaaaag aacctgaacg ccgggttatt ggtttcgtcg tggcaatcgt aacccaactc
    28441 attcaaccgg gtttcgaaat ccggttcatg gtcgccaagt tcgaacgccg ccagtacgcg
    28501 cccgtagtcg gtgccatggc tgcgatagtg gaacaaagaa atgttccagt gcgtacccag
    28561 cgtgttgagg aagcgcaaca gcgcgcccgg cgattccggg aattcgaagc tgtagaggcg
    28621 ttcctgcaac ggatgcgacg gacgcccgcc gaccatatag cgcacatgca gcttcgccat
    28681 ttcgtcgtcg gagagatcca ctacgctgta gccgccatcg ttgagcatct gcaaaatttc
    28741 tttgcgctct tcgaggccac ggctcaggcg cacaccgaca aagatgcagg cgtttttggc
    28801 atcggcaaaa cggtagttga actcggtgac cgagcgcccg ccaagcagct ggcagaactt
    28861 caggaagctg cccttctctt ccgggatggt taccgccagc aacgcttcac gctgttcgcc
    28921 cagttcgcaa cgttccgaga cgtagcgcag gccgtgaaag ttcacgttag caccagaaag
    28981 aatatgcgcc agccgctcac cgcgaatgtt gtgctgggcg atatattttt tcattcccgc
    29041 cagtgccagc gcgccagagg gttccgctac cgcgcgcaca tcttcgaaca ggtctttcat
    29101 cgccgcacat atggcatcgc tatcgacggt gatgatgtcg tcgagatact cctggcataa
    29161 acggaaggtt tcgtcgccga tgcgtttcac cgcaacacct tcggcaaaca gccccacgcg
    29221 tggcagatca accggatgac ctgcatccag cgccgctttc aggcaggcgg agtcttccgc
    29281 ttccacggca atcactttga tttgcggcat cagttgtttg atcagtaccg ccacgcccgc
    29341 cgccagacct ccgccgccga ctggcacaaa tacgcggtcg agatgggcgt cctgctggag
    29401 cagttccagc gccagcgtgc cttgcccggc gatcaccatc ggatggtcga acggcggcac
    29461 ccaggtgaag ccctgctgct gtgatagttc gatcgctttc gcttttgctt catcaaagtt
    29521 cgcgccgtga agaagcactt cgccgccgaa gcctcgcacc gcgtcgactt tgatatcggc
    29581 ggtggcggtt ggcatgacga tcagtgcctt cacgcctaac cgtgcggaag aaaacgcgac
    29641 gccctgcgcg tggttacccg cagaagcggt gatcacgccg tgcgctttct gttcttccgt
    29701 caggcccgcc atcatggcgt atgcgccgcg cagcttaaag ctatgaactg gctgacggtc
    29761 ttcgcgtttc accagaatca cgttatcgag acgcgacgag agtttttcca ttttttgtag
    29821 cggcgtgacc tgcgccgctt cgtagaccgg cgcgcgcagc accgctctta aatattcggc
    29881 gccttccggg gcgccggaga gaggttgtga gtctgccatc attaaccccc cagtttcgat
    29941 ttatcacgca ccgcgccttt gtcggcgctg gttgccaggc tggcgtaggc acgcagcgca
    30001 aaggaaacct gacgttcacg atttttcggt gtccaggctt tgttgccacg agcttcctgc
    30061 gcttcacgac gcgccgccag ttcggcatcg cttacctgta actgaatgcc acggttcgga
    30121 atgtcgatag cgatcaggtc accatcttca atcaggccaa tgctgccgcc acttgccgct
    30181 tccggtgaga cgtggccgat ggaaagacca gaggtgccgc cagagaaacg accgtcggtg
    30241 atcagcgcac aggctttacc gagacccatt gatttcagga aactggttgg gtagagcatt
    30301 tcctgcatcc ccggaccgcc tttcgggcct tcatagcgaa ttactaccac atctccggcg
    30361 acaactttac cgccgagaat cgcttctacc gcgtcgtcct ggctttcgta gactttcgcc
    30421 gggccagtga atttgaggat gctgtcatcg acgcctgcgg ttttaacgat gcagccgttt
    30481 tctgcgaaat taccgtagag caccgccagg ccgccgtctt tgctgtaggc gtgttccagc
    30541 gagcggatac agccattgga gcgatcgtcg tccagcgtat cccagcggca attctgcgag
    30601 aatgcctgtg tagtacgaat gcccgccggg ccagcgcgga acatattttt taccgcatca
    30661 tcctgggtca cgatgatgtc gtattgttcc agcgtttgcg gcaacgtcag gccaagtaca
    30721 tttttcacat cacggttcag taaccccgca cgatccagct cgccgagaat accgataaca
    30781 ccaccagcac ggtgaacatc ttccatatgg tatttttggg tgctcggcgc gactttacac
    30841 agctgcggta ctttgcggga gagcttatcg atatcactca tggtgaagtc gatttccgct
    30901 tcctgcgccg ccgccagtag gtgaagaacg gtgttggtag agccgcccat cgcgatatcc
    30961 agcgtcatgg cgttttcaaa cgccgcctta ctggcgatgt tacgcggcag tgcgctttca
    31021 tcatcctgtt cgtagtaacg tttagtcaat tcaacaatgc gtttaccagc attgaggaac
    31081 agttgcttgc gatcagcgtg ggttgccagc agcgagccgt tgcccggctg cgacaggccc
    31141 agcgcttcgg tcaggcagtt cattgagtta gcagtaaaca tcccagagca ggaaccgcag
    31201 gttgggcagg cggatcgttc aacctgatcg ctctgggagt cagaaacttt cgggtctgcg
    31261 ccctggatca tcgcatcaac cagatcgagc ttaatgatct gatcggacag tttggttttc
    31321 ccggcctcca tcgggccgcc ggaaacaaag atcaccggaa tattcaggcg cagggaagcc
    31381 atcagcatcc ccggggtgat tttgtcacag ttagagatgc agaccatggc gtcggcgcag
    31441 tgggcgttga ccatatattc aacggaatca gcgatcagtt cgcgagatgg cagtgaataa
    31501 agcatgcccc cgtggcccat agcgatccca tcatccaccg caatggtgtt gaactctttg
    31561 gcaacgccgc ccgccgcttc aatttgttcg gcgaccagtt taccgagatc gcgcagatgg
    31621 acgtgacccg gtacaaattg ggtgaacgag ttcacaaccg cgataatcgg cttaccgaaa
    31681 tcggcgtcgg tcattccggt ggcgcgccac agcgcacgag cacccgccat attacgacca
    31741 tgagtggtgg tggcggaacg gtacttaggc atactttatt tactcccagt gtctgtctcg
    31801 taaatgggac ggtgcgtgcc gtcccatttt ttgtatttat tgattaactt gatctaacca
    31861 gccccattta tcttcggtct cgccagtgaa gaggccgaag aaggcttgct gaatgcgttt
    31921 ggtaaccggg ccgcaacggc cttcaccaac ctgaatacca tctacactgc gcactggcgt
    31981 gatttctgcc gccgtaccgg acataaagac ttcatccgcc aggtacaggg attcgcgcga
    32041 cagcacctgc tcacgcactt caattcccag ctctttcgcc agtttgatga tggcatcacg
    32101 ggtaataccc ggcagcgcgg aggaggtgaa cggtggagtg aacagcacac cgtctttcac
    32161 ttcaaacagg ttttcgcctg cgccttcaga gatataacca ttcacatcca acgcgatacc
    32221 ttcctgataa ccgtggcggc gcgcttcgct acccaccaac agggaagaga ggtagttacc
    32281 accggctttc gctgcggttg ggatggtgtt tggtgcagca cggttccagg aggaaaccat
    32341 cgcatcgatc ccctgctcca gcgcttctgc gcccagatac gccccccacg ggaaagcggc
    32401 gataatcaca tcggttgagt atcccgctgg cgggtttacg cccatgccaa catcaccgac
    32461 gaaaatcagc ggacggatat aggcgctggt gagattgttt ttgcggatca cgtcacgaca
    32521 agcttccatc agctcatcaa tgctctgcga aaccgggaag cgatagattt tggcggagtc
    32581 atgcagacgc tgcatatgct cacgatggcg gaatacaacc ggtcctttgt gcgagtcgta
    32641 gcaacggatg ccttcaaaaa ccgaggtgcc atagtgcagc gcgtgcgaca tcacatgcac
    32701 cttcgcgtct tcccagcgaa ccatctcccc attgaaccaa atgtaatcag ctttcttcgt
    32761 ggtcattttt atattccttt tgcgctcagg cgcggatttg ttgtgatgta gttgtgctct
    32821 ggcagatagc aacgtgtgcg acgtccacca gtttatttaa ctgactaaac agtaagtcga
    32881 ccgaccgtgg gctggcaacg gtcaattcga tatttatatt ttgtgcatcg ctggcggcgg
    32941 ccatattcat tgagcagacg tggaaaccac gatgacgcac cacgcgtaaa acacgttcta
    33001 aggtttccgg attgaagcga gccgatacat tgacctgatg ttgcatcatg ataatttctc
    33061 caacatttca gaattactgg caccaggcgg caccagcggc cagacgttct caagttcgtc
    33121 gattgagaca tgaagcaggt atggcccatc actgttcagc atggtgtcga gtgccgcttc
    33181 aacctggtct ttatgggtga tgtgttggcc agggatgccg aaggcgctgg ctaacatgag
    33241 gaaatcgggg ttatcggtaa gggtggtttc gctgtatcgt tcctggaaaa acagttgctg
    33301 ccattgtcga accatcccta accgttggtt atcgagtaag acgattttca acggtaactg
    33361 cttgcgtttt acggtgccca gctcttgcac attcatcata aaagagccgt caccggagat
    33421 acagacgaca gtatcgttcg gtcgcgcgac ttgtgcgcca accgccgccg gtaaaccgaa
    33481 acccatggtg cctaagccgc tggaggtgat gaagttttcc gggcgagtgt ggacgatgtg
    33541 ctgggcagcc cacatctggt gctgccccac atctgtggtc acgatgcaat ccgcaggttt
    33601 acgatccgac agttgtttta acaacaacgg cgcgtagata gcgtcaccag gatggtcgta
    33661 acgccaggca tgttcatcac gcagttgcgc gcagtgttgc tgccagtcat cgatatttaa
    33721 cggctgctgt aatgctggta acagagcatt taaatcaccc tgtaatgcca catgtgcctg
    33781 acgcagcttg ttcatttctg ccggatcgat atccatatgg ataacgctgg cgtgtggtgc
    33841 gaaggtgttc agtttgccgg tcacccggtc atcaaaacgt gcgcccacgg ctatcagtag
    33901 atcgcactcc tgcactgcga agttcgccgc tttggtgccg tgcatcccca gcatgcccag
    33961 atagtacgga taatctgctt ctactgcgcc cagccctttc aacgtacagg tggcaggcat
    34021 ttttgtggca gcgagaaatt cacgcaaagc cgaaactgcc tgcgccatgc ccacgccacc
    34081 gccaacgtac agcatcggtt tttgcgcttt tgccagcatc tgacgcgctt gctcgacttc
    34141 ggcatgtggg aaagtcactt cgttttcaac ggtggtgaac cacggttcca gatcgccgct
    34201 ggctaactgg atatcttttg gaatatcgac caaaactgga ccaggacgac cagagctggc
    34261 gacgtcaaac gcttcggcca tgatacgcgg taactcttcc agtgattgca ccaggaaact
    34321 gtgcttggtg caggctaacg acaatcccag gatatccact tcctgaaatg cgtcggtgcc
    34381 gataaacgct gcggatacct gaccggtgat ggcaacaacg gggatggaat ctaacagagc
    34441 gtccgcaagc ccagttatca ggttggttgc acctggacca gacgtggcga tgcaaacgcc
    34501 agttttgccg gtagcacggg cataaccgat agccgccatt gctgcacctt gctcgtgtcg
    34561 gcacagcaag tgttccacgc caccgtcata caatgcatcg taaaccggca taattgcgcc
    34621 acccggataa ccgaaaacgg tgttcacacc ctgtgcccgc aacgcatgta ccacccactg
    34681 tgcgccattc atagttagtt ccccgtcctg aatcttgaga aacagaattt tgtgctgtta
    34741 ttcattgtct gctcctcagt tatgttttta aggtcaaaaa aaacccccgg acctttcggt
    34801 gcgggggtct tagttcgtta aggcttgatc tctaagcctt tcctcgtcca agtgcagccc
    34861 cgcacggtgg gataataatc accaccacgc taatcacgac caggctaatc actcgtagaa
    34921 gggctgtcat tttgtctttt cttgcatctt gttcgaagga atgcctaaag agttaccata
    34981 gattttgcga atagcacaag atattttttg gccgatt
//
LOCUS       DAHHTG010000041        34458 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.041, whole genome shotgun sequence.
ACCESSION   DAHHTG010000041 DAHHTG010000000
VERSION     DAHHTG010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 34458)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34458)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34458
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.041"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            215..1189
                     /gene="hemB"
                     /locus_tag="NBI74_001940"
     CDS             215..1189
                     /gene="hemB"
                     /locus_tag="NBI74_001940"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HCD1932285.1"
                     /translation="MTDLIQRPRRLRKSPALRAMFEETTLSLNDLVLPIFVEEEIDDY
                     KAVEAMPGVMRIPEKHLAREIERIANAGIRSVMTFGISHHTDETGSDAWREDGLVARM
                     SRICKQTVPEMIVMSDTCFCEYTSHGHCGVLCEHGVDNDATLENLGKQAVVAAAAGAD
                     FIAPSAAMDGQVQAIRQALDAAGFKDTAIMSYSTKFASSFYGPFREAAGSALKGDRKS
                     YQMNPMNRREAIRESLLDEAQGADCLMVKPAGAYLDIVRELRERTELPIGAYQVSGEY
                     AMIKFAALAGAIDEEKVVLESLGSIKRAGADLIFSYFALDLAEKKILR"
     gene            complement(1229..2080)
                     /gene="tauD"
                     /locus_tag="NBI74_001941"
     CDS             complement(1229..2080)
                     /gene="tauD"
                     /locus_tag="NBI74_001941"
                     /EC_number="1.14.11.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine dioxygenase"
                     /protein_id="HCD1932286.1"
                     /translation="MSERLSITPLGPYIGAQITGADLTRPLSDNQFEQLYHAVLRHQV
                     VFLRDQAITPQQQRALAQRFGELHIHPVYPHAEGVDEIIVLDTHNDNPPDNDNWHTDV
                     TFIQTPPAGAILAAKELPSTGGDTLWASGIAAYEALSVPFRQLLSGLRAEHDFRKSFP
                     EYKYRKTEAEHQRWREAVAKNPPLLHPVVRTHPVTGKQALFVNEGFTTRIVDVSEKES
                     EALLGFLFAHITKPEFQVRWRWQPNDIAIWDNRVTQHYANADYLPQRRIMHRATILGD
                     IPFYRAG"
     gene            complement(2077..2904)
                     /gene="tauC"
                     /locus_tag="NBI74_001942"
     CDS             complement(2077..2904)
                     /gene="tauC"
                     /locus_tag="NBI74_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter permease TauC"
                     /protein_id="HCD1932287.1"
                     /translation="MSVLINEKLHSHRLKWRWPLSRQVTLSIGTLAVLLTVWWAVAAL
                     QLISPLFLPPPQQVLAKLLTIAGPQGFMDATLWQHLAASLTRILLALLAAVLIGIPVG
                     IAMGLSPTVRGILDPIIELYRPVPPLAYLPLMVIWFGIGETSKILLIYLAIFAPVAMS
                     ALAGVKSAQQVRIRAAQSLGASRAQVLWFVILPGALPEILTGLRIGLGVGWSTLVAAE
                     LIAATRGLGFMVQSAGEFLATDVVLAGIAVIAIIAFLLELGLRALQRRLTPWHGEVQ"
     gene            complement(2901..3668)
                     /gene="tauB"
                     /locus_tag="NBI74_001943"
     CDS             complement(2901..3668)
                     /gene="tauB"
                     /locus_tag="NBI74_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter ATP-binding subunit"
                     /protein_id="HCD1932288.1"
                     /translation="MLQISHLYADYGGKPALEDINLTLESGELLVVLGPSGCGKTTLL
                     NLIAGFVPYQHGSIQLAGKGIQGPGAERGVVFQNEGLLPWRNVQDNVAFGLQLAGVEK
                     MQRLEIAHQMVKKVGLEGAEKRYIWQLSGGQRQRVGIARALAANPQLLLLDEPFGALD
                     AFTRDQMQTLLLKLWQETGKQVLLITHDIEEAVFMATELVLLSPGPGRVLERLPLNFA
                     RRFVAGESSRSIKSDPQFIAMREYVLSRVFEQREAFS"
     gene            complement(3681..4643)
                     /gene="tauA"
                     /locus_tag="NBI74_001944"
     CDS             complement(3681..4643)
                     /gene="tauA"
                     /locus_tag="NBI74_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter substrate-binding
                     protein"
                     /protein_id="HCD1932289.1"
                     /translation="MAISSRNTFLAALAFIAFQAQAVNVTVAYQTSAEPAKVAQADNT
                     FAKESGATVDWRKFDSGASIVRALASGDVQIGNLGSSPLAVATSQQVPIEVFLLASKL
                     GNSEALVVKKTISKPEDLIGKRIAVPFISTTHYSLLAALKHWGIKPGQVEIVNLQPPA
                     IIAAWQRGDIDGAYVWAPAVNALEKDGKVLTDSEQVGQWGAPTLDVWVVRKDFAEKHP
                     EVVKAFAKSAIDAQQPYIANPDAWLKQPENISKLARLSGVPEGDVPGLVKGNTYLTPQ
                     QQTAELTGPVNKAIIDTAQFLKEQGKVPAVANDYSQYVTSRFVQ"
     gene            4949..5224
                     /gene="frmR"
                     /locus_tag="NBI74_001945"
     CDS             4949..5224
                     /gene="frmR"
                     /locus_tag="NBI74_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414891.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formaldehyde-responsive transcriptional
                     repressor FrmR"
                     /protein_id="HCD1932290.1"
                     /translation="MPSTPEEKKKVLTRVRRIRGQIDALERSLEGDAECRAILQQIAA
                     VRGAANGLMAEVLESHIRETFDRNDCYSREVSQSVDDTIELVRAYLK"
     gene            5259..6368
                     /gene="frmA"
                     /locus_tag="NBI74_001946"
     CDS             5259..6368
                     /gene="frmA"
                     /locus_tag="NBI74_001946"
                     /EC_number="1.1.1.284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-(hydroxymethyl)glutathione dehydrogenase"
                     /protein_id="HCD1932291.1"
                     /translation="MKSRAAVAFAPGKPLEIVEIDVAPPKKGEVLIKVTHTGVCHTDA
                     FTLSGDDPEGVFPVVLGHEGAGVVVEVGEGVTSVKPGDHVIPLYTAECGECEFCRSGK
                     TNLCVAVRETQGKGLMPDGTTRFSYNGQPLYHYMGCSTFSEYTVVAEVSLAKINPEAN
                     HEHVCLLGCGVTTGIGAVHNTAKVQPGDSVAVFGLGAIGLAVVQGARQAKAGRIIAID
                     TNPKKFELARRFGATDCINPNDYDKPIKDVLLDINKWGIDHTFECIGNVNVMRAALES
                     AHRGWGQSVIIGVAGSGQEISTRPFQLVTGRVWKGSAFGGVKGRSQLPGMVEDAMKGD
                     IDLEPFVTHTMSLDEINDAFDLMHEGKSIRTVIRY"
     gene            6461..7294
                     /gene="fghA"
                     /locus_tag="NBI74_001947"
     CDS             6461..7294
                     /gene="fghA"
                     /locus_tag="NBI74_001947"
                     /EC_number="3.1.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HCD1932292.1"
                     /translation="MELIEKHASFGGWQNVYRHYSQSLKCEMNVGVYLPPKAENEKLP
                     VLYWLSGLTCNEQNFITKSGMQRYAAEHNIIVVAPDTSPRGSHVADADRYDLGQGAGF
                     YLNATQAPWNEHYKMYDYIRNELPNLVMHHFPATARKSIAGHSMGGLGALVLALRNPD
                     EYVSVSAFSPIVSPSQVPWGQQAFAAYLGENKDAWLDYDPVSLISQGQRVAEIMVDQG
                     LSDDFYAEQLRTPNLEKICQEMNIKTLIRYHEGYDHSYYFVSSFIGEHIAYHANKLNM
                     R"
     gene            complement(7419..7958)
                     /locus_tag="NBI74_001948"
     CDS             complement(7419..7958)
                     /locus_tag="NBI74_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2058 domain-containing protein"
                     /protein_id="HCD1932293.1"
                     /translation="MAKLTLQEQLLKAGLVTSKKAAKVERTAKKSRVQAREARAAVEE
                     NKKAQLERDKQLSEQQKQAALAKEYKAQVKQLIEMNRITIANGDIGFNFTDGNLIKKI
                     FVDKLTQTQLINGRLAIARLLVDNNSEGEYAIIPASVADKIAQRDASSIVLHSALSAE
                     EQDEDDPYADFKVPDDLMW"
     gene            complement(8060..9271)
                     /gene="mhpT"
                     /locus_tag="NBI74_001949"
     CDS             complement(8060..9271)
                     /gene="mhpT"
                     /locus_tag="NBI74_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-(3-hydroxy-phenyl)propionate transporter MhpT"
                     /protein_id="HCD1932294.1"
                     /translation="MSTRTPSSSSSRLMLTIGLCFLVALMEGLDLQAAGIAAGGIAQA
                     FALDKMQMGWIFSAGILGLLPGALVGGMLADRYGRKRILIGSVALFGLFSLATAIAWD
                     FPSLVFARLMTGVGLGAALPNLIALTSEAAGPRFRGTAVSLMYCGVPIGAALAATLGF
                     AGANLAWQTVFWVGGVVPLILVPLLMRWLPESTVFAGEKQAAPPLRALFAPETATATL
                     LLWLCYFFTLLVVYMLINWLPLLLVEQGFQPSQAAGVMFALQMGAASGTLMLGALMDK
                     LRPVTMSLLIYSGMLASLLALGTVSSFNSMLLAGFVAGLFATGGQSVLYALAPLFYSS
                     QIRATGVGTAVAVGRLGAMSGPLLAGKMLALGTGTVGVMAASAPGILVAGLAVFILMS
                     RRSRMQPCADA"
     gene            complement(9447..10460)
                     /gene="dmpG"
                     /locus_tag="NBI74_001950"
     CDS             complement(9447..10460)
                     /gene="dmpG"
                     /locus_tag="NBI74_001950"
                     /EC_number="4.1.3.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-2-oxovalerate aldolase"
                     /protein_id="HCD1932295.1"
                     /translation="MNGKKLYISDVTLRDGMHAIRHQYSLENVRQIAKALDDARVDSI
                     EVAHGDGLQGSSFNYGFGAHSDLEWIEAAADVVKHAKIATLLLPGIGTIHDLKNAWQA
                     GARVVRVATHCTEADVSAQHIQYARELGMDTVGFLMMSHMTTPENLAKQAKLMEGYGA
                     TCIYVVDSGGAMNMSDIRDRFRALKAVLKPETQTGMHAHHNLSLGVANSIAAVEEGCD
                     RIDASLAGMGAGAGNAPLEVFIAAADKLGWQHGVDLYALMDAADDLVRPLQDRPVRVD
                     RETLALGYAGVYSSFLRHCETAAARYGLSAVDILVELGKRRMVGGQEDMIVDVALDLR
                     NNK"
     gene            complement(10457..11407)
                     /gene="mhpF"
                     /locus_tag="NBI74_001951"
     CDS             complement(10457..11407)
                     /gene="mhpF"
                     /locus_tag="NBI74_001951"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetaldehyde dehydrogenase"
                     /protein_id="HCD1932296.1"
                     /translation="MSKRKVAIIGSGNIGTDLMIKILRHGQHLEMAVMVGIDPQSDGL
                     ARARRMGVATTHEGVIGLMNMPEFADIDIVFDATSAGAHVKNDAALREAKPDIRLIDL
                     TPAAIGPYCVPVVNLEENVAQLNVNMVTCGGQATIPMVAAVSRVARVHYAEIIASIAS
                     KSAGPGTRANIDEFTETTSRAIEVVGGAAKGKAIIVLNPAEPPLMMRDTVYVLSDEAS
                     QDDIEASINEMAEAVQAYVPGYRLKQRVQFEVIPQDKPVNLPGVGQFSGLKTAVWLEV
                     EGAAHYLPAYAGNLDIMTSSALATAEKMAQSLARKAGEAA"
     gene            complement(11404..12213)
                     /gene="mhpD"
                     /locus_tag="NBI74_001952"
     CDS             complement(11404..12213)
                     /gene="mhpD"
                     /locus_tag="NBI74_001952"
                     /EC_number="4.2.1.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-4-pentenoate hydratase"
                     /protein_id="HCD1932297.1"
                     /translation="MTKHTLEQLAADLRRAAEQGEAIAPLRDLIGIDNAEAAYAIQHI
                     NVQHDVAQGRRVVGRKVGLTHPKVQQQLGVDQPDFGTLFADMCYGDNETIPFSRVLQP
                     RIEAEIALVLNRDLPATDITFDELYNAIEWVLPALEVVGSRIRDWSIQFVDTVADNAS
                     CGVYVIGGPAQRPAGLDLKNCAMKMTRNNEEVSSGRGSECLGHPLNAAVWLARKMASL
                     GEPLRAGDIILTGALGPMVAVNAGDRFEAHIEGIGSVAATFSSAAPKGSLS"
     gene            complement(12223..13089)
                     /gene="mhpC"
                     /locus_tag="NBI74_001953"
     CDS             complement(12223..13089)
                     /gene="mhpC"
                     /locus_tag="NBI74_001953"
                     /EC_number="3.7.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414883.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-6-oxonona-2,4-dienedioate hydrolase"
                     /protein_id="HCD1932298.1"
                     /translation="MSYQPQTEAATSRFLNVEEAGKTLRIHFNDCGQGDETVVLLHGS
                     GPGATGWANFSRNIDPLVEAGYRVILLDCPGWGKSDSIVNSGSRSDLNARILKSVVDQ
                     LDIAKIHLLGNSMGGHSSVAFTLNWPERVGKLVLMGGGTGGMSLFTPMPTEGIKRLNQ
                     LYRQPTIENLKLMMDIFVFDTSDLTDALFEARLNNMLSRRDHLENFVKNLEANPKQFP
                     DFGPRLAEIKAQTLIVWGRNDRFVPMDAGLRLLSGIAGSELHIFRDCGHWAQWEHADA
                     FNQLVLNFLARP"
     gene            complement(13107..14051)
                     /gene="mhpB"
                     /locus_tag="NBI74_001954"
     CDS             complement(13107..14051)
                     /gene="mhpB"
                     /locus_tag="NBI74_001954"
                     /EC_number="1.13.11.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-carboxyethylcatechol 2,3-dioxygenase"
                     /protein_id="HCD1932299.1"
                     /translation="MHAYLHCLSHSPLVGYVDPAQEVLDEVNGVIASARERIAAFSPE
                     LVVLFAPDHYNGFFYDVMPPFCLGVGATAIGDFGSAAGELPVPVELAEACAHAVMKSG
                     IDLAVSYCMQVDHGFAQPLEFLLGGLDKVPVLPVFINGVATPLPGFQRTRMLGEAIGR
                     FTSTLNKRVLFLGSGGLSHQPPVPELAKADAHMRDRLLGSGKDLPASERELRQQRVIS
                     AAEKFVEDQRTLHPLNPIWDNQFMTLLEQGRIQELDAVSNEELSAIAGKSTHEIKTWV
                     AAFAAISAFGNWRSEGRYYRPIPEWIAGFGSLSARTEN"
     gene            complement(14053..15717)
                     /locus_tag="NBI74_001955"
     CDS             complement(14053..15717)
                     /locus_tag="NBI74_001955"
                     /EC_number="1.14.13.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3-(3-hydroxy-phenyl)propionate/3-hydroxycinnamic acid
                     hydroxylase"
                     /protein_id="HCD1932300.1"
                     /translation="MAIQHPDIQPAVNHSVQVAIAGAGPVGLMMANYLGQMGIDVLVV
                     EKLDKLIDYPRAIGIDDEALRTMQSVGLVDNVLPHTTPWHAMRFLTPKGRCFADIQPM
                     TDEFGWPRRNAFIQPQVDAVMLEGLSRFPNVRCLFSRELEAFSQQDDEVTLHLKTEEG
                     QRETVKAQWLVACDGGASFVRRTLNVPFEGKTAPNQWIVVDIANDPLSTPHIYLCCDP
                     VRPYVSAALPHAVRRFEFMVMPGETEEQLREPQNMRKLLSKVLPNPDNVELIRQRVYT
                     HNARLAQRFRIDRVLLAGDAAHIMPVWQGQGYNSGMRDAFNLAWKLALVIQGKARDAL
                     LDTYQQERRDHAKAMIDLSVTAGNVLAPPKRWQGTLRDGVSWLLNYLPPVKRYFLEMR
                     FKPMPQYYGGALVREGEAKHSPVGKMFIQPKVTLENGDVTLLDNAIGANFAVIGWGCN
                     PLWGMSDEQIQQWRALGTRFIQVVPEVQIHTAQDNHDGVLRVGDTQGRLRSWFAQHNA
                     SLVVMRPDRFVAATAIPQTLGKTLNKLASVMTLTRPDADVSVEKVA"
     gene            15908..16741
                     /gene="mhpR"
                     /locus_tag="NBI74_001956"
     CDS             15908..16741
                     /gene="mhpR"
                     /locus_tag="NBI74_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator MhpR"
                     /protein_id="HCD1932301.1"
                     /translation="MQNNEQTEYKTVRGLTRGLMLLNMLNKLDGGASVGLLAELSGLH
                     RTTVRRLLETLLEEGYVRRSTSDDSFRLTIKVRQLSEGFRDEQWISALAAPLLGDLLR
                     EVVWPTDVSTLDVDAMVVRETTHRFSRLSFHRAMVGRRLPLLKTASGLTWLAFCPEQE
                     RKELIEMLASRPGDDYQLAREPLKLEAILARARKEGYGQNYRGWDQEEKIASIAVPLR
                     SEQRVIGCLNLVYMASAMTIEQAAEKHLPALQRVAKQIEEGVESQAILVAGRRSGVHL
                     R"
     gene            16818..17900
                     /gene="lacI"
                     /locus_tag="NBI74_001957"
     CDS             16818..17900
                     /gene="lacI"
                     /locus_tag="NBI74_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414879.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor LacI"
                     /protein_id="HCD1932302.1"
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQACHVSAKTREKVEAAMAE
                     LNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERS
                     GVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACANVPALFLDVSDQTPINSII
                     FSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPITEREG
                     DWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDT
                     EDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLPPNT
                     QTASPRALADSLMQLARQVSRLESGQ"
     gene            18023..21097
                     /gene="lacZ"
                     /locus_tag="NBI74_001958"
     CDS             18023..21097
                     /gene="lacZ"
                     /locus_tag="NBI74_001958"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HCD1932303.1"
                     /translation="MTMITDSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEAR
                     TDRPSQQLRSLNGEWRFAWFPAPEAVPESWLECDLPDADTVVVPSNWQMHGYDAPIYT
                     NVTYPITVNPPFVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWV
                     GYGQDSRLPSEFDLSAFLRAGENRLAVMVLRWSDGSYLEDQDMWRMSGIFRDVSLLHK
                     PTTQISDFQVTTRFNDDFSRAVLEAEVQMYGELRDELRVTVSLWQGETQVASGTAPFG
                     GEIIDERGGYADRVTLRLNVENPELWSAEIPNLYRAVVELHTADGTLIEAEACDVGFR
                     EVRIENGLLQLNGKPLLIRGVNRHEHHPLHGQVMDEQTMVQDILLMKQNNFNAVRCSH
                     YPNHPLWYTLCDRYGLYVVDEANIETHGMVPMNRLTDDPRWLPAMSERVTRMVQRDRN
                     HPSVIIWSLGNESGHGANHDALYRWIKSVDPSRPVQYEGGGADTSATDIICPMYARVD
                     EDQPFPAVPKWSIKKWLSLPGEIRPLILCEYAHAMGNSLGGFAKYWQAFRQYPRLQGG
                     FVWDWVDQSLIKYDENGNPWSAYGGDFGDTPNDRQFCMNGLVFADRTPHPALTEAKHQ
                     QQYFQFRLSGRTIEVTSEYLFRHSDNEFLHWMVALDGKPLASGEVPLDVGPQGKQLIE
                     LPELPQPESAGQLWLTVRVVQPNATAWSEAGHISAWQQWRLAENLSVTLPSASHAIPQ
                     LTTSETDFCIELGNKRWQFNRQSGFLSQMWIGDEKQLLTPLRDQFTRAPLDNDIGVSE
                     ATRIDPNAWVERWKAAGHYQAEAALLQCTADTLADAVLITTAHAWQHQGKTLFISRKT
                     YRIDGHGEMVVSVGVEVASDTPHPARIGLTCQLAQVAERVNWLGLGPQENYPDRLTAA
                     CFNRWDLPLSDMYTPYVFPSENGLRCGTRELNYGPHQWRGDFQFNISRYSQQQLMETS
                     HRHLLHAEEGTWLNIDGFHMGIGGDDSWSPSVSAEFQLSAGRYHYQLVWCQK"
     gene            21149..22402
                     /gene="lacY"
                     /locus_tag="NBI74_001959"
     CDS             21149..22402
                     /gene="lacY"
                     /locus_tag="NBI74_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactose permease"
                     /protein_id="HCD1932304.1"
                     /translation="MYYLKNTNFWMFGLFFFFYFFIMGAYFPFFPIWLHDINHISKSD
                     TGIIFAAISLFSLLFQPLFGLLSDKLGLRKYLLWIITGMLVMFAPFFIFIFGPLLQYN
                     ILVGSIVGGIYLGFCFNAGAPAVEAFIEKVSRRSHFEFGRARMFGCVGWALCASIVGI
                     MFTINNQFVFWLGSGCALILAVLLFFAKTDAPSSATVANAVGANHSAFSLKLALELFR
                     QPKLWFLSLYVIGVSCTYDVFDQQFANFFTSFFATGEQGTRVFGYVTTMGELLNASIM
                     FFAPLIINRIGGKNALLLAGTIMSVRIIGSSFATSALEVVILKTLHMFEVPFLLVGCF
                     KYITSQFEVRFSATIYLVCFCFFKQLAMIFMSVLAGNMYESIGFQGAYLVLGLVALGF
                     TLISVFTLSGPGPLSLLRRQVNEVA"
     gene            22468..23079
                     /gene="lacA"
                     /locus_tag="NBI74_001960"
     CDS             22468..23079
                     /gene="lacA"
                     /locus_tag="NBI74_001960"
                     /EC_number="2.3.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactoside O-acetyltransferase"
                     /protein_id="HCD1932305.1"
                     /translation="MNMSMTERIKAGKLFTDMCEGLPEKRLRGKTLMYEFNHSHPSEF
                     EKRESLIKEMFATVGENAWVEPPVYFSYGSNIHIGRNFYANFNLTIVDDYTVTIGDNV
                     LIAPNVTLSVTGHPVHHELRKNGEMYSFPITIGNNVWIGSHVVINPGITIGDNSVIGA
                     GSVVTKDIPPNVVAAGVPCRIIREINDRDKQYYFKDFKVESSV"
     gene            complement(23200..24483)
                     /gene="codA"
                     /locus_tag="NBI74_001961"
     CDS             complement(23200..24483)
                     /gene="codA"
                     /locus_tag="NBI74_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine/isoguanine deaminase"
                     /protein_id="HCD1932306.1"
                     /translation="MSNNALQTIINARLPGKEGLWQIHFHDGKISAIDAQSGVMPVTE
                     NSLDAEQGLVLPPFVEPHIHLDTTQTAGQPNWNQSGTLFEGIERWAERKALLTHDDVK
                     QRAWQTLKWQIANGIQHVRTHVDVSDATLTALKAMLEVKQEVAPWIDLQIVAFPQEGI
                     LSYPNGEALLEEALRLGADVVGAIPHFEFTREYGVESLHKTFALAQKYDRLIDVHCDE
                     IDDEQSRFVETVAALAHREGMGARVTASHTTAMHSYNGAYTSRLFRLLKMSGINFVAN
                     PLVNIHLQGRFDTYPKRRGITRVKEMLESGINVCFGHDDVFDPWYPLGTANMLQVLHM
                     GLHVCQLMGYGQINDGLNLITHHSARTLNLQDYGIAAGNSANLIILPAENGFDALRRQ
                     VPVRYSVRGGKVIASTKPAQTTVYLEQPEAIDYKR"
     gene            complement(24473..25732)
                     /gene="codB"
                     /locus_tag="NBI74_001962"
     CDS             complement(24473..25732)
                     /gene="codB"
                     /locus_tag="NBI74_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine permease"
                     /protein_id="HCD1932307.1"
                     /translation="MSQDNNFSQGPVPQSARKGVLALTFVMLGLTFFSASMWTGGTLG
                     TGLSYHDFFLAVLIGNLLLGIYTSFLGYIGAKTGLTTHLLARFSFGVKGSWLPSLLLG
                     GTQVGWFGVGVAMFAIPVGKATGLDINLLIAVSGLLMTVTVFFGISALTVLSVIAVPA
                     IACLGGYSVWLAVNGMGGLDALKAVVPAQPLDFNVALALVVGSFISAGTLTADFVRFG
                     RNAKLAVLVAMVAFFLGNSLMFIFGAAGAAALGMADISDVMIAQGLLLPAIVVLGLNI
                     WTTNDNALYASGLGFANITGMSSKTLSVINGIIGTVCALWLYNNFVGWLTFLSAAIPP
                     VGGVIIADYLMNRRRYEHFATTRMMSVNWVAILAVALGIAAGHWLPGIVPVNAVLGGA
                     LSYLILNPILNRKTTAAMTHVEANSVE"
     gene            complement(26057..27943)
                     /gene="prpE"
                     /locus_tag="NBI74_001963"
     CDS             complement(26057..27943)
                     /gene="prpE"
                     /locus_tag="NBI74_001963"
                     /EC_number="6.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionate--CoA ligase"
                     /protein_id="HCD1932308.1"
                     /translation="MSFSEFYQRSINEPEQFWAEQARRIDWQTPFTQTLDHSNPPFAR
                     WFCEGRTNLCHNAIDRWLEKQPEALALIAVSSETEEERTFTFRQLHDEVNAVASMLRS
                     LGVQRGDRVLVYMPMIAEAHITLLACARIGAIHSVVFGGFASHSVAARIDDAKPVLIV
                     SADAGARGGKIIPYKKLLDDAISQAQHQPRHVLLVDRGLAKMARVSGRDVDFASLRHQ
                     HIGARVPMAWLESNETSCILYTSGTTGKPKGVQRDVGGYAVALATSMETIFGGKAGGV
                     FFCASDIGWVVGHSYIVYAPLLAGMATIVYEGLPTWPDCGVWWKIVEKYQVSRMFSAP
                     TAIRVLKKFPTAEIRKHDLSSLEVLYLAGEPLDEPTASWVSNTLDVPVIDNYWQTESG
                     WPIMAIARGLDDRPTRLGSPGVPMYGYNVQLLNEVTGEPCGVNEKGMLVVEGPLPPGC
                     IQTIWGDDDRFVKTYWSLFSRPVYATFDWGIRDADGYHFILGRTDDVINVAGHRLGTR
                     EIEESISSHPGVAEVAVVGVKDALKGQVAVAFVIPKESDSLEDREVAHSQEKAIMALV
                     DSQIGNFGRPAHVWFVSQLPKTRSGKMLRRTIQAICEGRDPGDLTTIDDPASLDQIRQ
                     AMEE"
     gene            complement(27983..29434)
                     /gene="prpD"
                     /locus_tag="NBI74_001964"
     CDS             complement(27983..29434)
                     /gene="prpD"
                     /locus_tag="NBI74_001964"
                     /EC_number="4.2.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylcitrate dehydratase"
                     /protein_id="HCD1932309.1"
                     /translation="MSAQINNIRPEFDREIVDIVDYVMNYEISSRVAYDTAHYCLLDT
                     LGCGLEALEYPACKKLLGPIVPGTVVPNGVRVPGTQFQFDPVQAAFNIGAMIRWLDFN
                     DTWLAAEWGHPSDNLGGILATADWLSRNAVASGKAPLTMKQVLTGMIKAHEIQGCIAL
                     ENSFNRVGLDHVLLVKVASTAVVAEMLGLTREEILNAVSLAWVDGQSLRTYRHAPNTG
                     TRKSWAAGDATSRAVRLALMAKTGEMGYPSALTAPVWGFYDVSFKGESFRFQRPYGSY
                     VMENVLFKISFPAEFHSQTAVEAAMTLYEQMQAAGKTAADIEKVTIRTHEACIRIIDK
                     KGPLNNPADRDHCIQYMVAIPLLFGRLTAADYEDNVAQDKRIDALREKINCFEDPAFT
                     ADYHDPEKRAIANAITLEFTDGTRFEEVVVEYPIGHARRRQDGIPKLVDKFKINLARQ
                     FPTRQQQRILEVSLDRTRLEQMPVNEYLDLYVI"
     gene            complement(29468..30637)
                     /gene="prpC"
                     /locus_tag="NBI74_001965"
     CDS             complement(29468..30637)
                     /gene="prpC"
                     /locus_tag="NBI74_001965"
                     /EC_number="2.3.3.16"
                     /EC_number="2.3.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylcitrate synthase"
                     /protein_id="HCD1932310.1"
                     /translation="MSDTTILQNSTHVIKPKKSVALSGVPAGNTALCTVGKSGNDLHY
                     RGYDILDLAEHCEFEEVAHLLIHGKLPTRDELAAYKTKLKALRGLPANVRTVLEALPA
                     ASHPMDVMRTGVSALGCTLPEKEGHTVSGARDIADKLLASLSSILLYWYHYSHNGERI
                     QPETDDDSIGGHFLHLLHGEKPSQSWEKAMHISLVLYAEHEFNASTFTSRVIAGTGSD
                     MYSAIIGAIGALRGPKHGGANEVSLEIQQRYETPDEAEADIRKRVENKEVVIGFGHPV
                     YTIADPRHQVIKRVAKQLSQEGGSLKMYNIADRLETVMWESKKMFPNLDWFSAVSYNM
                     MGVPTEMFTPLFVIARVTGWAAHIIEQRQDNKIIRPSANYVGPEDRQFVALDKRQ"
     gene            complement(30797..31687)
                     /gene="prpB"
                     /locus_tag="NBI74_001966"
     CDS             complement(30797..31687)
                     /gene="prpB"
                     /locus_tag="NBI74_001966"
                     /EC_number="4.1.3.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylisocitrate lyase"
                     /protein_id="HCD1932311.1"
                     /translation="MPLHSPGKAFRAALSKETPLQIVGTINANHALLAQRAGYQTIYL
                     SGGGVAAGSLGLPDLGISTLDDVLTDIRRITDVCSLPLLVDADIGFGSSAFNVARTVK
                     SMIKAGAAGLHIEDQVGAKRCGHRPNKAIVSKEEMVDRIRAAVDAKTDPDFVIMARTD
                     ALAVEGLDAAIERAQAYVEAGAEMLFPEAITELAMYRQFADAVQVPILANITEFGATP
                     LFTTDELRSAHVAMALYPLSAFRAMNRAAEHVYNILRQEGTQKSVIDTMQTRNELYES
                     INYYQYEEKLDDLFARGQVK"
     gene            31926..33511
                     /gene="prpR"
                     /locus_tag="NBI74_001967"
                     /pseudo
     CDS             31926..33511
                     /gene="prpR"
                     /locus_tag="NBI74_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414864.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="propionate catabolism operon regulatory protein
                     PrpR"
     gene            complement(33612..33887)
                     /locus_tag="NBI74_001968"
     CDS             complement(33612..33887)
                     /locus_tag="NBI74_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HCD1932312.1"
                     /translation="MKIISKMLVGALAFAVTNVYAAELMTKAEFEKVASQYEKIGDIS
                     TSNEMSTADAKEDLIKKADEKGADVLVLTSGQTDNKIHGTANIYKKK"
     gene            34034..>34458
                     /locus_tag="NBI74_001969"
     CDS             34034..>34458
                     /locus_tag="NBI74_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HCD1932313.1"
                     /translation="MMKLLHLFMDEITMDPLHAVYLTVGLFVITFFNPGANLFVVVQT
                     SLASGRRAGVLTGLGVALGDAFYSGLGLFGLATLITQCEEIFSLIRIVGGAYLLWFAW
                     CSMRRQSTPQMSTLQQPISAPWYIFFRRGLITDLSNPQTV"
BASE COUNT         7991 a         9607 c         8992 g         7868 t
ORIGIN      
        1 aacgtcacca aatccccaaa aacaataatc tatggctata aataaaaatt aaaaatattt
       61 tcagtgaata tgaaattatt gctatttcta ttacaaagat gtcctgacct ctttttcatt
      121 ctcttcttgt caacatcgcg acaactttcg taaaacacac ccagcctgct tcaggtatac
      181 tatgcccctc gattccacaa acatcaggca gaccatgaca gacttaatcc aacgccctcg
      241 tcgcctgcgc aaatctcctg cgctgcgcgc tatgtttgaa gagacaacac ttagccttaa
      301 cgacctggtg ttgccgatct ttgttgaaga agaaattgac gactataaag ccgttgaagc
      361 catgccaggc gtgatgcgca ttccagagaa acatctggca cgcgaaattg aacgcatcgc
      421 caacgccggt attcgttccg tgatgacttt cggcatctct caccataccg atgaaaccgg
      481 cagcgatgcc tggcgggaag atggactggt ggcgcgtatg tcgcgcatct gcaagcagac
      541 cgtgccagaa atgatcgtta tgtcagacac ctgcttctgt gaatacacat ctcacggtca
      601 ctgcggtgtt ctgtgcgagc atggcgtcga caacgacgcg actctggaaa atttaggcaa
      661 gcaagccgtg gttgcagctg ctgcaggcgc agacttcatc gccccttctg ccgcgatgga
      721 tggtcaggta caggcgattc gccaggcgct ggacgctgcg ggctttaaag atacggcgat
      781 tatgtcgtat tcgaccaagt tcgcctcctc cttttatggc ccgttccgtg aagctgccgg
      841 aagcgcatta aaaggcgacc gcaaaagcta tcagatgaac ccaatgaacc gtcgtgaggc
      901 gattcgtgag tcactgctgg atgaagccca gggcgcagac tgcctgatgg ttaaacctgc
      961 cggagcatac ctcgacatcg tgcgtgagct gcgtgaacgt actgaattgc cgattggcgc
     1021 gtatcaggtg agcggtgagt acgcgatgat taagttcgcc gcgctggcgg gtgctataga
     1081 tgaagagaaa gtcgtgctcg aaagcttagg ttcgattaag cgtgcgggtg cggatctgat
     1141 tttcagctac tttgcgctgg atttggctga gaagaagatt ctgcgttaat gatcattatg
     1201 ccgggttatc caacccggca tttccccctt accccgcccg ataaaacgga atatccccaa
     1261 gaatcgtcgc ccgatgcata atccgccgct gtggcaggta atccgcattg gcatagtgct
     1321 gagtcactcg gttatcccaa atcgcaatat cattcggttg ccagcgccag cgcacctgaa
     1381 actccggttt agtgatatgg gcaaacaaaa aacctaacaa ggcttcgctc tctttctcgc
     1441 tgacatcaac aattcgtgtg gtaaaacctt cgttcacaaa cagtgcctgt ttaccggtca
     1501 ccggatgcgt tcgcaccacc ggatgtagca acggtggatt tttcgcgacc gcctcacgcc
     1561 agcgttgatg ctcagcctcg gttttgcggt acttgtattc cgggaacgat ttacggaaat
     1621 catgctccgc acgcaacccg ctcaacagct ggcggaaggg aacagagagc gcctcatatg
     1681 cggcaatacc actggcccag agcgtatcgc cgccggtcga gggtaactct ttcgccgcca
     1741 gaatcgcccc tgcgggtggc gtttgaataa atgtcacatc ggtgtgccag ttgtcgttat
     1801 ccggcggatt atcattatgg gtatccagga cgatgatctc gtcaacccct tcagcatgag
     1861 ggtaaacagg gtgaatatgc agctcgccaa aacgctgggc cagcgcgcgt tgctgctgcg
     1921 gcgtgatagc ttgatcgcgc agaaacacca cctgatggcg cagcaccgca tggtaaagct
     1981 gttcaaactg attatcgctt aacgggcgcg ttaagtcggc ccccgtaatt tgtgcgccaa
     2041 tatacggccc cagcggggta atgctcagac gttcactcat tgtacttctc catgccaggg
     2101 cgtcaggcgg cgctgtaacg cgcgcagacc cagttctaaa agaaaggcga taatcgcaat
     2161 caccgcgatc cccgccagca ccacgtcagt cgcgagaaat tcacccgctg actgaaccat
     2221 aaatcctaaa ccgcgcgttg cggcaatcag ctccgccgcc accagcgtag accagcccac
     2281 acccagacca atgcgtaatc cggtgaggat ttccggcagc gcgccgggca aaatgacaaa
     2341 ccacagcacc tgcgcacggc tggcacccag cgactgggcg gcacgaatgc gaacctgctg
     2401 cgcgcttttc acccccgcca gcgccgacat cgccaccggc gcaaaaatcg ctaaatagat
     2461 cagcaagatc ttcgaggttt cgccaatacc aaaccagatc accatcagcg gcaaataagc
     2521 cagcggcggc accggacgat aaagctcgat tatcggatcc agaatgccgc gcaccgtcgg
     2581 gcttagcccc atggcgatcc cgaccggaat accgatcaac accgccgcga gtagcgccag
     2641 caaaatgcgc gtcaggctgg ctgccagatg ctgccacagc gtggcgtcca taaatccctg
     2701 cggcccggca atggtgagca gttttgccag tacctgttgc ggtggcggca aaaacagcgg
     2761 gctaatcagt tgcagcgccg cgactgccca ccatacagtg agcaaaaccg ctaacgtacc
     2821 aatgcttaag gtcacctgac gcgagagcgg ccagcgccat ttcagccgat gcgaatgcag
     2881 tttttcatta atgagcacac tcatgagaac gcctcccgtt gctcaaatac gcggcttaaa
     2941 acatattcgc gcatggcgat gaattgtgga tcggacttga tgctgcggct cgactctccc
     3001 gcaacaaagc ggcgagcaaa gttgagcggc agccgctcca gcacacggcc tgggccgggt
     3061 gaaagcaaaa ccagttcagt cgccataaac accgcttctt ctatatcgtg ggtaatcaac
     3121 agcacctgct ttcccgtctc ctgccaaagt ttcagcagca gggtttgcat ctggtcgcgg
     3181 gtgaaggcgt ccagcgcacc aaacggttcg tcgagtaata acagctgggg atttgccgcc
     3241 aacgcgcggg caatccccac ccgctgacgt tgaccaccgg aaagctgcca gatgtagcgt
     3301 ttttctgcgc cttccagccc gacttttttc accatctggt gtgcgatttc cagtcgctgc
     3361 attttctcta cacctgccag ttgcaggccg aacgccacgt tgtcctgtac attgcgccac
     3421 ggtaacaacc cttcattctg aaaaactacg ccacgctctg ctcccggtcc ctgaataccc
     3481 ttacccgcca gttgaatgct gccatgctga taaggcacaa aaccagcaat caaattcagc
     3541 aacgtagttt tgccgcagcc agacggcccc agaactacca gaagctcgcc gctttccagc
     3601 gtcaggttga tatcttccag tgccggtttg ccgccataat cggcgtaaag atgagaaatt
     3661 tgcagcatct gcgcctcctt ttattgcacg aagcgcgagg taacgtactg gctgtaatca
     3721 ttcgctacag ccgggacttt gccctgctct ttcaaaaact gcgcggtgtc gatgatcgct
     3781 ttgttcaccg gtccggtcag ttctgccgtt tgttgctgcg gcgtcagata ggtattcccc
     3841 ttcaccagcc ccggaacgtc accttccggc acgccgctta accgcgccag tttgctgatg
     3901 ttttccggct gtttcagcca cgcgtctggg ttagcaatgt acggttgctg agcatcgatg
     3961 gcgcttttag cgaacgcttt cacgacctca ggatgtttct cggcaaaatc tttccgcacg
     4021 acccagacgt ccagtgtcgg cgcgccccac tgcccgacct gttcagaatc ggtcagcacc
     4081 ttgccatctt tttccagggc gttaaccgcc ggtgcccata cataagcacc atcaatatct
     4141 ccccgctgcc atgcggcgat aattgcgggc ggctgcaggt tcacaatctc aacttgccca
     4201 ggtttaatac cccagtgttt cagcgccgcc agcaggctgt agtgggtggt ggagataaac
     4261 ggtacggcga tgcgtttacc aatcaagtct tccggtttac tgatagtttt ctttaccacc
     4321 agcgcttcgg agttacccag ttttgacgcc agcaagaaga cttcaatcgg cacctgttgg
     4381 ctggttgcaa ccgctaacgg gctggaaccg aggttgccga tttgcacatc gcccgaagcc
     4441 agcgcccgca cgatgctggc tccactgtca aacttacgcc agtccacggt tgctccgctt
     4501 tctttagcaa aggtgttgtc ggcctgagcc actttcgccg gttcggcgga ggtttgatac
     4561 gccacggtga cgttcaccgc ttgcgcctga aaagcgatga atgccagtgc ggcaagaaat
     4621 gtgttacgcg atgaaattgc catgattgtc tgctcccccg tcttgttatg ggagcagtat
     4681 ttcgttgcgg gtaagtttga taaaggaatt aaaacgtctt gttaataaca aagcgttttt
     4741 agaaaaaaat cggcaatggt tagcgagttt gcgcattttg ctacttttaa caaacatcgc
     4801 ctgatgtgac gctcacgcct cttatcatgc ctacaaatac tggggccgga taaattttat
     4861 tctccagtgt tatatactat aggggggtat gcattgacat atagcatacc cccctatagt
     4921 atattgcgtg cagatgatga ggtgcgaaat gcccagtact ccggaagaga agaaaaaggt
     4981 ccttactcga gttcgtcgta ttcgggggca gattgatgct ctggaacggt cgctggaggg
     5041 tgatgccgaa tgccgtgcca tactccaaca gatcgctgcc gttcggggcg cggctaacgg
     5101 actgatggca gaagtgcttg aaagccatat ccgggaaacg tttgaccgaa atgactgcta
     5161 cagccgtgaa gtcagccaat ccgttgacga cactattgaa ctggttcgtg cctatcttaa
     5221 atagctgaat ctattaccat attgaggaag agcgagagat gaaatcacgt gctgccgttg
     5281 catttgctcc cggtaaaccg ctggaaatcg ttgaaattga cgttgcacca ccgaaaaaag
     5341 gtgaagtgct gattaaagtc acccataccg gtgtttgcca taccgacgca tttaccctct
     5401 ccggcgatga cccggaaggt gtcttcccag tggttctcgg tcacgaaggg gccggggttg
     5461 tagttgaagt cggtgaaggc gtaaccagcg tcaaacctgg cgaccatgtg atcccacttt
     5521 acaccgcaga gtgcggcgag tgtgagtttt gtcgttctgg caaaaccaac ctgtgtgtcg
     5581 cggttcgcga aacgcagggc aaaggcttga tgccagacgg caccacccgt ttttcttaca
     5641 acgggcagcc gctttatcac tacatgggat gctcaacatt cagtgaatac accgtggtag
     5701 cggaagtgtc tctggccaaa attaatccag aagcaaacca tgaacacgtc tgcctgctgg
     5761 gctgtggcgt gaccaccggt attggcgcgg tacacaacac agctaaagtc cagccaggtg
     5821 attctgttgc cgtgtttggt cttggcgcga ttggtctggc agtggttcag ggcgcgcgtc
     5881 aggcaaaagc gggacggatt atcgctatcg ataccaaccc gaagaaattc gaactggcgc
     5941 gtcgcttcgg tgccaccgac tgcattaacc cgaatgacta cgacaaaccg attaaagatg
     6001 tcctgctgga tatcaacaaa tggggtatcg accatacctt tgaatgcatc ggtaacgtca
     6061 acgtgatgcg tgcagcgctg gaaagcgcgc accgcggctg gggtcagtcg gtgatcatcg
     6121 gggtagcagg ctccggtcag gaaatctcca cccgtccatt ccagttggtc accggtcgcg
     6181 tatggaaagg ttccgcgttt ggcggcgtga aaggtcgttc ccagttaccg ggtatggttg
     6241 aagatgcgat gaaaggtgat atcgatctgg aaccgtttgt cacgcatacc atgagcctgg
     6301 atgaaattaa tgacgccttc gacctgatgc atgaaggtaa atccattcga accgtaattc
     6361 gttactgatt tcccgctggt ttaccccgtc cagttcagat ggggttctta atactctccc
     6421 cgggcagccg tccgggggat taaccgtgag ataatgactg atggaactca ttgaaaaaca
     6481 tgccagcttt ggcggctggc aaaatgtgta tcggcattat tcccaatcac tgaaatgtga
     6541 aatgaatgtc ggcgtctatc tccccccaaa agccgagaat gaaaaattgc cggtgttgta
     6601 ctggctttca ggcctgacct gcaacgagca aaatttcatt actaaatcgg ggatgcagcg
     6661 ttacgcggct gagcacaaca ttattgttgt tgcgccggac accagtccgc gaggcagtca
     6721 tgtcgcagat gctgaccgtt acgatctcgg gcaaggtgcc gggttttacc tgaacgcgac
     6781 gcaagcgccg tggaatgaac attacaaaat gtatgactat atccgcaacg agctgccgaa
     6841 tttagtgatg catcattttc cggcaacggc cagaaagtca atcgcaggtc attctatggg
     6901 cgggctgggc gcgctggtgc tggcgttacg taacccagat gaatatgtca gcgtctcggc
     6961 gttttcgccc attgtctccc catcgcaagt accgtgggga cagcaagcct ttgccgcata
     7021 tcttggtgaa aataaagatg cctggttgga ttacgatccg gtgagtctta tttcacaagg
     7081 tcaacgcgtt gcggaaatca tggttgatca ggggttgagt gatgattttt acgcggaaca
     7141 actgcggact ccaaatcttg aaaagatctg ccaggagatg aatatcaaga cgttaatccg
     7201 ttatcacgag ggttatgatc acagctatta ttttgtctcc agttttattg gcgagcatat
     7261 tgcctaccac gccaataaac tgaatatgcg ttgagcatcc gcgcacgcat gccggatgcg
     7321 gcgtaaacct tatccggccc acatttcgct cgtaaaccgt aggcctgata agacgcgaaa
     7381 agcgtcgcat caggcatctg cacacgattt atcaatgttt accacatcaa atcatcaggc
     7441 actttgaagt cggcatacgg atcgtcttca tcctgctctt cggcgctgag cgcgctgtgc
     7501 aacacaatac tgctggcatc acgctgagca attttatcgg caacgctggc gggaataatg
     7561 gcgtattcac cttcgctatt gttatcgacc aacaagcggg caatcgccag acgaccatta
     7621 atcaactgcg tttgcgtgag cttatcgaca aaaatcttct taatcagatt gccgtcagtg
     7681 aagttaaaac caatatcgcc attggcaatg gtaattcggt tcatttcaat gagctgcttc
     7741 acctgggctt tatattcttt cgccaacgcc gcttgttttt gttgctcgct aagctgttta
     7801 tcacgctcaa gctgtgcctt tttattttct tctaccgccg cccgtgcttc acgcgcctga
     7861 acacgcgatt ttttcgccgt tctctcgacc ttcgccgctt ttttgctggt gactagtccc
     7921 gctttgagca actgctcttg taaggtaagt tttgccatct ttgtttccaa acccgctgaa
     7981 taatttttag cgagtatacc tgtaaacgcc gtaggtgtat tgggggcaag tccctttcgc
     8041 ccctgcttaa taaggcgcgt caggcatcgg cacacggctg cattcttgat ctccgactca
     8101 tcaaaataaa caccgccaac ccagcaacaa gaatacccgg tgcagaagcg gccattacgc
     8161 cgaccgtgcc agtgcctaat gccagcattt tcccggccag taacggaccg ctcatagccc
     8221 ccagacgccc taccgccacg gctgttccca cacctgttgc gcggatctgc gaactgtaaa
     8281 acaacggtgc cagagcatac aaaacgcttt gcccacctgt cgcaaacaac cccgcgacaa
     8341 atcccgccag caacatactg ttaaatgacg acaccgttcc cagcgccagc agcgaagcta
     8401 acatgccgct ataaatcagt agcgacatgg ttactggacg cagtttatcc atcaatgcgc
     8461 ccaacattaa cgtcccgctt gccgccccca tttgcagtgc aaacatcacc cctgccgcct
     8521 gcgatggctg gaatccttgc tccaccaaaa gcagcggcag ccagttgatc aacatgtaga
     8581 ccaccagcag agtgaaaaaa taacacaacc acagcagcag cgtcgcggtt gccgtttctg
     8641 gcgcaaataa cgcacgcagt ggtggcgcag cctgtttttc gccagcgaaa accgtcgact
     8701 ccggcagcca gcgcattaat agcggcacca gaatcaacgg caccacacca cctacccaaa
     8761 acaccgtttg ccatgctaag tttgcccccg cgaaacccag cgtcgccgcc agcgccgcgc
     8821 caatgggaac accgcaatac atcaggctca ctgccgtccc acgaaaacgt ggccccgcgg
     8881 cttcagacgt cagggcgata agattcggca acgccgcccc cagcccgaca ccggtcatca
     8941 gccgcgcaaa gaccagtgag gggaaatccc atgcaatcgc cgttgccagc gagaacaaac
     9001 caaacagcgc aactgagcca atcaaaatac gcttacgccc ataacggtct gccagcatcc
     9061 cgccaaccag cgcgccgggt agcaaaccga gtattccggc gctaaatatc cagcccattt
     9121 gcattttatc gagtgcaaaa gcctgggcga tgccacccgc cgcaatgcca gccgcctgaa
     9181 gatccagccc ttccatcaga gcgaccaaaa aacaaagccc gatggtcagc atcaggcggg
     9241 atgaagatga tgaaggggta cgagtcgaca taagatgaac ctcattgcga gtgcaggtga
     9301 atcgcgactg atacattgcc agccgcgtaa tcagaactat ttaatgactg cctacgggcg
     9361 aaatgtaggc cggataaggc gttcacgccg catccggcag tcgtgctggg atgggcatac
     9421 aaaatgcacg ttctcggcag tcaatattat ttgttgttgc gcagatccag cgccacgtcg
     9481 acgatcatat cctcctggcc gccaaccatc cggcgtttgc ccagctcaac gagaatatcc
     9541 accgcgctta agccataacg cgccgccgcc gtttcacagt gacgcaggaa gctggagtaa
     9601 acaccagcgt atcccagcgc cagcgtttcg cgatcgactc gtaccggtcg atcctgcaac
     9661 ggacgcacca ggtcgtcagc ggcatccatt aacgcataga gatcgacccc atgctgccaa
     9721 cccagtttat ccgcggcggc aataaatact tccagcggcg cgttacctgc gcccgcgccc
     9781 attcccgcga ggctggcgtc gattcggtcg cagccctctt ccaccgccgc gatagagttc
     9841 gccacgccaa gactcaggtt atggtgagcg tgcatgccgg tttgcgtttc tggtttcagc
     9901 actgctttca gggcgcggaa acggtcacgg atatcgctca tgttcatcgc accgccagaa
     9961 tccaccacat aaatacaggt cgcaccgtag ccttccatca actttgcctg ctttgcgaga
    10021 ttctccggcg tggtcatatg gctcatcatc agaaaaccaa cggtgtccat tccgagctcg
    10081 cgggcatact gaatatgctg ggcggaaaca tcagcttcgg tacagtgcgt tgccacacga
    10141 accacccgcg cgccagcctg ccaggcattt ttcagatcgt gaatagtgcc gattcctggc
    10201 agcaacaacg tcgcgatttt ggcgtgcttc accacatccg ccgccgcttc aatccattca
    10261 aggtcgctat gtgcgccgaa accatagtta aagctggaac cctgcaaacc gtcgccgtgg
    10321 gccacttcaa tcgaatccac gcgggcatca tccagtgctt tggcaatctg gcggacattt
    10381 tccagcgaat actgatgacg aatggcgtgc ataccgtcac gcaacgtgac gtccgaaata
    10441 taaagttttt taccgttcat gccgcttctc ctgccttgcg cgccagtgac tgggccattt
    10501 tttccgctgt cgccagcgca ctggaagtca taatgtcgag gttgcccgca taggcaggca
    10561 gataatgcgc tgcgccttcg acttccagcc agaccgccgt tttcagcccg gagaactgcc
    10621 ccacgcccgg taaattgacc ggtttatcct gcgggatgac ttcaaactgc acgcgctgtt
    10681 tcaggcgata acccggtacg taagcctgca ccgcctcagc catttcattg attgaggctt
    10741 cgatatcatc ttgtgaagct tcgtcgctca atacatacac cgtgtcacgc atcatcaacg
    10801 gtggctctgc tgggttaagc acaataatcg ccttcccttt tgccgcgcca cccaccactt
    10861 caatggcgcg cgaagtggtt tccgtaaatt catcgatgtt ggcacgcgtg ccgggtccgg
    10921 cagatttact ggcaatagaa gcgataattt cggcgtaatg aacacgcgcc acgcgtgaaa
    10981 ctgccgctac cattggaatg gtggcctggc cgccgcaggt gaccatgttg acgttcagtt
    11041 gagcgacgtt ctcctcgagg ttaaccaccg gcacgcagta agggccgatg gcagcaggcg
    11101 tcaggtcaat taagcgaata tccggtttcg cttcgcgcaa agcggcatcg tttttcacat
    11161 gcgcgcccgc gctggtcgca tcaaatacaa tgtcgatatc agcaaattca ggcatgttca
    11221 tcagcccgat caccccttca tgggtggtgg cgacgcccat acgtctggca cgcgccagac
    11281 cgtcggactg aggatcaatg ccgaccatca ccgccatctc cagatgctga ccgtgacgca
    11341 aaattttaat catcagatcg gtgccaatgt tgcctgaacc gataatggcg actttacgct
    11401 tactcatgac agacttcctt ttggggctgc gcttgaaaat gtcgcagcaa ctgaacctat
    11461 gccttcgata tgggcttcaa aacgatcgcc cgcattcacc gccaccatcg gacctaatgc
    11521 cccggtaaga atgatatctc cggcgcgcag cggttcaccc agactggcca ttttgcgtgc
    11581 cagccagacg gcggcattaa gcggatgtcc caggcattcg ctgccgcgcc cgctggaaac
    11641 ctcttcgtta ttacgcgtca tcttcatggc gcagtttttc aggtctaacc ccgccgggcg
    11701 ttgcgccgga ccgccgatga catacacccc gcaggaagcg ttatctgcca cggtatcgac
    11761 aaactgaatc gaccagtcgc gaatgcggct ccccaccacc tccagcgccg gaagtaccca
    11821 ttcaatggcg ttatacaatt cgtcgaaggt gatatcggtt gcgggtagat cgcggttcaa
    11881 caccagtgca atctccgctt caatgcgggg ttgcagaaca cgggaaaaag gaatggtttc
    11941 attatcgcca taacacatgt cggcaaataa tgtcccgaaa tccggttgat caacgcccag
    12001 ttgttgctgc actttcggat gcgtcagccc cactttgcgc cccaccacgc gacgcccctg
    12061 cgcaacgtca tgttgcacat tgatgtgctg aatggcgtag gccgcttcag cgttatcgat
    12121 accaatcaga tcgcgcagcg gcgcaatcgc ttcgccctgc tctgcggcgc ggcgtaaatc
    12181 cgccgccagt tgctcaagag tatgcttcgt catgaccgtt ccttaagggc gtgcgaggaa
    12241 attcagcacc agttgattga aagcgtcggc atgttcccac tgcgcccagt gaccacagtc
    12301 gcggaagata tgcagttcag aaccggcaat gccggacagc agacgcagac ccgcatccat
    12361 cggcacaaag cggtcgttgc gcccccagac aatcagggtt tgcgctttga tttccgccag
    12421 acgtgggcca aaatccggga actgtttcgg attggcttcc aggttcttaa cgaagttttc
    12481 caggtgatcg cggcgcgaca gcatattatt caggcgcgct tcaaacaggg cgtcggtcaa
    12541 atcgctggta tcaaaaacga agatatccat catcagcttc aggttttcga tagtcggctg
    12601 acgataaagc tgattcagtc gcttaatacc ttcggttggc atcggcgtaa acaaactcat
    12661 gccgcccgta ccgccgccca tcagcaccag tttgccgacg cgctccggcc agttaagagt
    12721 gaacgccaca gaactgtggc cacccatcga gttgcccagc aggtggattt tggcgatatc
    12781 cagttgatcc accacgcttt tcaggattcg cgcattaaga tccgatcgcg aaccactatt
    12841 aacgatcgaa tcgctcttac cccaacccgg acaatccagc aggatcaccc gatagcccgc
    12901 ctctaccagc ggatcgatat tgcggctgaa gttcgcccag ccagtagcac ccgggccgga
    12961 accatgcagc aggacaacgg tttcgtcgcc ttgtccgcag tcattaaaat ggatgcgcag
    13021 cgttttaccc gcttcttcta cattcagaaa acggctggtg gcggcttcgg tttgtggctg
    13081 ataactcatc atcttctcct gcatattcag ttctctgttc tggcgcttaa cgagccaaat
    13141 ccggcaatcc actccgggat tgggcggtaa taacgccctt cgctacgcca gttgccaaac
    13201 gcagaaatag cggcaaaagc ggcgacccag gttttgattt catgtgtcga ctttccggca
    13261 atggcggaaa gctcttcgtt actgacggca tccagttcct gtatgcgtcc ctgctccagc
    13321 aaagtcatga actggttatc ccaaatcggg ttgagcggat gcagcgttct ctgatcctca
    13381 acaaacttct cagcggcgct aatcacccgt tgctgacgca attcgcgctc actggcgggt
    13441 aaatctttcc cgctccccaa cagacggtcg cgcatatggg catcggcttt cgccagttcg
    13501 ggcaccggcg gctgatggga aagcccaccg gaacccagga acagcacgcg tttattgaga
    13561 gtactggtga aacgtccaat ggcttccccc aacatgcggg tacgctggaa accgggcagc
    13621 ggcgtggcga caccgttgat gaacaccggc agtactggca ccttatccag cccaccgagc
    13681 aggaactcca gcggctgggc gaacccgtgg tccacctgca tacagtaaga aacggcaaga
    13741 tcgatcccgc tcttcatgac ggcatgcgca caggcctccg ccagctccac aggcacgggc
    13801 agctctcctg ccgcactgcc gaaatcacca attgccgtcg ctccaacgcc taaacagaac
    13861 ggtggcatca cgtcatagaa aaagccgttg tagtgatctg gcgcaaacag caccaccagt
    13921 tcaggggaga aagccgcaat acgctcgcgg gcgctggcaa tcacgccatt gacctcatcg
    13981 agtacctctt gtgccgggtc gacatacccc accagcggcg agtgggaaag acagtgaaga
    14041 taagcgtgca tatcaggcta ccttttcgac agaaacgtcg gcatcagggc gggtcagcgt
    14101 catcaccgac gccagtttat tcagggtctt gcccagggtt tgcggaatgg cggtggcggc
    14161 aacaaagcga tccgggcgca tcaccaccag cgaagcattg tgttgcgcga accagctacg
    14221 caggcgacct tgcgtatccc ccacgcgtag tacgccgtcg tggttatcct gtgcggtatg
    14281 aatttgcact tccggcacca cctgaatgaa gcgtgtgccc aacgcgcgcc actgctggat
    14341 ttgctcgtcg ctcatccccc acagtggatt gcatccccag ccaattaccg cgaagttcgc
    14401 gccgatcgcg ttatcgagta gcgtcacgtc gccgttttcc agcgtgactt tcggctgaat
    14461 aaacatcttg ccgaccggag agtgcttcgc ttcgccctca cgcaccagcg caccgccgta
    14521 atattgcggc atcggcttga agcgcatttc gaggaagtag cgttttactg gcggcagata
    14581 attcagcagc caggaaacgc cgtcacgtaa cgtaccctgc cagcgtttcg gcggagccag
    14641 cacgttgccc gccgtcacgg acaggtcaat catcgctttg gcgtgatcgc gacgttcttg
    14701 ttgataggta tcgagcagcg catcgcgggc tttcccctgg ataaccaacg ccagtttcca
    14761 tgcgaggtta aaggcgtcgc gcataccact gttatagccc tgcccctgcc acaccggcat
    14821 gatgtgcgcg gcatcgcccg ccagcagtac gcgatcaata cgaaaacgtt gcgccagtcg
    14881 cgcgttgtgg gtgtagacac gctggcgaat caattcaaca ttgtccggat taggcagcac
    14941 tttgcttaac agcttgcgca tattttgcgg ctcacgcagc tgttcttcgg tttctcccgg
    15001 catcaccata aattcaaagc gacgtaccgc atgaggcagc gcggcagaaa catacgggcg
    15061 caccggatca caacacaaat agatatgcgg cgtacttaac ggatcgttgg cgatatctac
    15121 cacaatccac tgatttggcg cagttttacc ttcaaacggc acattcaggg tgcgacggac
    15181 aaaacttgcc ccaccatcac aggccaccag ccactgggct ttgaccgttt cccgctgccc
    15241 ttcttccgtt ttcaggtgca aggtcacttc gtcatcttgc tggctgaagg cctccagctc
    15301 gcgggaaaac aggcagcgca cattcggaaa acgcgacaat ccttccagca tcaccgcatc
    15361 gacctgaggc tgaataaagg cgttacggcg cggccagcca aattcatcgg tcattggctg
    15421 aatatcagca aaacagcgac cttttggggt gagaaaacgc atcgcgtgcc acggcgtagt
    15481 gtgcggcaga acattatcga ccaggccaac cgactgcatg gtgcgcagcg cctcgtcatc
    15541 aataccaatc gcacgcgggt agtcgatcaa cttatcgagt ttctccacca ccagcacgtc
    15601 aatgcccatc tggccgagat agttcgccat catcagcccg accggaccgg caccagcgat
    15661 cgccacctga acgctatggt taacagcagg ctggatgtca gggtgttgta ttgccattcc
    15721 agtacctcac gactcggaca aaatgccgtt gcgcgcacag tacagcgcaa cttattttgt
    15781 taaaaacatg taaatgattt tttattgtgc gctcagtata ggaagggtgt tttcggctac
    15841 aatcaaaaca tgcccgaatg tgcaccaggt gcacaacgtt gttttaacta tataaatgtc
    15901 aattaatatg cagaacaatg agcagacgga atacaaaacc gtgcgcggct taacccgcgg
    15961 tctaatgtta ttaaatatgt taaataaact tgatggcggt gccagcgtcg ggctgctggc
    16021 ggaactcagc ggcctgcatc gcaccactgt gcggcgactg ctggagacgt tactggaaga
    16081 gggatatgtc cggcgcagta cctccgatga cagttttcgt ctgaccatca aagtgcggca
    16141 attaagcgaa ggatttcgtg acgaacagtg gatttctgca ctggcggccc cgctgctggg
    16201 cgatctgttg cgcgaagtgg tatggccgac agatgtgtcc acgctggatg ttgatgcaat
    16261 ggtggtacgc gaaaccaccc atcgtttcag ccgcttatct tttcaccggg caatggtcgg
    16321 gcgacggtta ccgcttctga aaaccgcctc gggcctgacc tggctggcct tttgcccgga
    16381 acaagaacgc aaggaattaa tcgagatgtt agcctcccgc cccggtgatg actatcaact
    16441 ggcacgagaa ccgttaaagc tggaagccat tctggcgcgc gcgcgcaaag aggggtacgg
    16501 acagaactac cgcggctggg atcaggagga gaagatcgcc tctatcgccg taccgctgcg
    16561 cagtgaacaa cgggtgattg gctgtctgaa tctggtgtat atggcgagcg caatgaccat
    16621 cgaacaggca gcggaaaagc atcttccggc gctgcaacgg gtggcaaaac agatcgaaga
    16681 aggggttgaa tcgcaggcta ttctggtagc gggaaggcga agcggcgtgc atttacgctg
    16741 acaccatcga atggcgcaaa acctttcgtg gtatggcatg atagcgcccg gaagagagtc
    16801 aattcagggt ggtgaatgtg aaaccagtaa cgctatacga tgtcgcagag tatgccggtg
    16861 tctcttatca gaccgtttcc cgcgtggtga accaggcctg ccacgtttca gcgaaaacgc
    16921 gggaaaaagt ggaagcggcg atggcggagc tgaattacat tcccaaccgc gtggcacaac
    16981 aactggcggg caaacagtcg ttgctgattg gcgttgccac ttccagtctg gccctgcacg
    17041 cgccgtcgca aattgttgcg gcgataaaat ctcgcgccga tcaactgggt gccagcgtgg
    17101 tggtgtcgat ggtagaacga agcggcgtcg aagcctgtaa agcggcggtg cacaatcttc
    17161 tcgcgcaacg cgtcagtggg ctgatcatta actatccgct ggatgaccag gatgccattg
    17221 ctgtggaagc tgcctgcgct aatgttccgg cgttatttct tgatgtctct gaccagactc
    17281 ccatcaacag tattattttc tcccatgaag acggtacgcg actgggcgtg gagcacctgg
    17341 tcgcattggg tcaccagcaa atcgcgctgt tagcaggccc attaagttct gtctcggcgc
    17401 gtctgcgtct ggcgggctgg cataaatatc tcacacgcaa tcaaattcag ccgataacag
    17461 aacgggaagg cgactggagt gccatgtccg gttttcaaca aaccatgcaa atgctaaatg
    17521 agggcatcgt tcccactgcg atgctggttg ccaacgatca gatggcgctg ggcgcaatgc
    17581 gcgccattac cgagtccggg ctgcgcgttg gtgcggatat ctcggtagtg ggatacgacg
    17641 ataccgaaga cagctcgtgt tatatcccgc cgttaaccac catcaaacag gattttcgcc
    17701 tgctggggca aaccagcgtg gaccgcttgc tgcaactctc tcagggccag gcggtgaagg
    17761 gcaatcagct gttgcccgtc tcactggtga aaagaaaaac cacccttccg cccaatacgc
    17821 aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcacga caagtttccc
    17881 gactggaaag cgggcagtga gcgcaacaca attaatgtga gtcagctcac tcattaggca
    17941 ccccagtctt tacactctat gtgtccggct cgtatgttgt gtgaaattgt gagcggataa
    18001 caatttcaca caggatacag ctatgactat gattacggat tctctggccg tcgtattaca
    18061 acgtcgtgac tgggaaaacc ctggcgttac acaacttaat cgccttgcgg cacatccccc
    18121 tttcgccagc tggcgaaata gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg
    18181 cagcctgaat ggcgaatggc gctttgcctg gtttccggca ccagaagcgg tgccggaaag
    18241 ctggctggag tgcgatcttc ctgacgccga tactgtcgtc gtcccctcaa actggcagat
    18301 gcacggttac gacgcgccca tctacaccaa cgtaacctat cccattacgg tcaatccgcc
    18361 gtttgttccc acggagaatc cgacaggttg ttactcgctc acatttaatg ttgatgaaag
    18421 ctggctacag gaaggccaga cgcgaattat ttttgatggc gttaactcgg cgtttcatct
    18481 gtggtgcaac gggcgctggg tcggttacgg ccaggacagc cgtttgccgt ctgaatttga
    18541 cctgagcgca tttttacgcg ccggagaaaa ccgcctcgcg gtgatggtgc tgcgctggag
    18601 tgacggcagt tatctggaag atcaggatat gtggcggatg agcggcattt tccgtgacgt
    18661 ctcgttgctg cataaaccga ccacgcaaat cagcgatttc caagttacca ctcgctttaa
    18721 tgatgatttc agccgcgcgg tactggaggc agaagttcag atgtacggcg agctgcgcga
    18781 tgaactgcgg gtgacggttt ctttgtggca gggtgaaacg caggtcgcca gcggcaccgc
    18841 gcctttcggc ggtgaaatta tcgatgagcg tggcggttat gccgatcgcg tcacgctacg
    18901 cctgaacgtt gaaaatccgg aactgtggag cgccgaaatc ccgaatctct atcgtgcggt
    18961 ggttgaactg cacaccgccg acggcacgct gattgaagca gaagcctgcg acgtcggttt
    19021 ccgcgaggtg cggattgaaa atggtctgct gcagctgaac ggcaagccgt tgctgattcg
    19081 cggcgttaac cgtcacgagc atcatcctct gcatggtcag gtcatggatg agcagacgat
    19141 ggtacaggat atcctgctga tgaagcagaa caactttaat gccgtgcgct gttcgcatta
    19201 tccgaaccat ccgctgtggt acacgctgtg cgaccgctac ggcctgtatg tggtggatga
    19261 agccaatatt gaaacccacg gcatggtgcc aatgaatcgt ctgaccgatg atccgcgctg
    19321 gctacccgcg atgagcgaac gcgtaacgcg gatggtgcag cgcgatcgta atcacccgag
    19381 tgtgatcatc tggtcgctgg ggaatgagtc aggccacggc gctaatcacg acgcactcta
    19441 tcgctggatc aaatctgtcg atccttcccg cccggtacag tatgaaggcg gcggagccga
    19501 cacctccgca accgatatta tttgcccgat gtacgcgcgc gtggatgaag accagccctt
    19561 cccggcggtg ccgaaatggt ccatcaaaaa atggctttcg ctgcctggag aaatacgccc
    19621 gctgatcctt tgcgaatatg cccacgcgat gggtaacagt cttggcggct tcgctaaata
    19681 ctggcaggcg tttcgtcagt acccccgttt acagggcggc ttcgtctggg actgggtgga
    19741 tcagtcgctg attaaatatg atgaaaacgg caacccgtgg tcggcttacg gcggtgattt
    19801 tggcgatacg ccgaacgatc gccagttctg tatgaacggt ctggtctttg ccgaccgcac
    19861 gccacatccg gcgctgacgg aagcaaaaca ccaacagcag tatttccagt tccgtttatc
    19921 cgggcgaacc atcgaagtga ccagcgaata cctgttccgt catagcgata acgagttcct
    19981 gcactggatg gtggcgctgg atggcaagcc gctggcaagc ggtgaagtgc ctctggatgt
    20041 tggcccgcaa ggtaagcagt tgattgaact gcctgaactg ccgcagccgg agagcgccgg
    20101 acaactctgg ctaacggtac gcgtagtgca accaaacgcg accgcatggt cagaagccgg
    20161 acacatcagc gcctggcagc aatggcgtct ggcggaaaac ctcagcgtga cactcccctc
    20221 cgcgtcccac gccatccctc aactgaccac cagcgaaacg gatttttgca tcgagctggg
    20281 taataagcgt tggcaattta accgccagtc aggctttctt tcacagatgt ggattggcga
    20341 tgaaaaacaa ctgctgaccc cgctgcgcga tcagttcacc cgtgcgccgc tggataacga
    20401 cattggcgta agtgaagcga cccgcattga ccctaacgcc tgggtcgaac gctggaaggc
    20461 ggctggccat taccaggccg aagcggcgtt gttgcagtgc acggcagata cacttgccga
    20521 cgcggtgctg attacgaccg cccacgcgtg gcagcatcag gggaaaacct tatttatcag
    20581 ccggaaaacc taccggattg atggacacgg cgagatggtc gtcagtgtag gtgttgaagt
    20641 agcgagcgat acaccgcatc cggcgcggat tggcctgacc tgccagctgg cgcaggtcgc
    20701 agagcgggta aactggctcg gcctggggcc gcaagaaaac tatcccgacc gccttactgc
    20761 agcctgtttt aaccgctggg atctgccatt gtcagacatg tataccccgt acgtcttccc
    20821 gagcgaaaac ggtctgcgct gcgggacgcg cgaattgaat tatggcccac accagtggcg
    20881 cggcgacttc cagttcaaca tcagccgcta cagccaacaa caactgatgg aaaccagcca
    20941 tcgccatctg ctgcacgcgg aagaaggcac atggctgaat atcgacggtt tccatatggg
    21001 gattggtggc gacgactcct ggagcccgtc agtatcggcg gaattccagc tgagcgccgg
    21061 tcgctaccat taccagttgg tctggtgtca aaaataataa taaccgggca ggccatgtct
    21121 gcccgtattt cgcgtaagga aatccattat gtactattta aaaaacacaa acttttggat
    21181 gttcggttta ttctttttct tttacttttt tatcatggga gcctacttcc cgtttttccc
    21241 gatttggcta catgacatca accatatcag caaaagtgat acgggtatta tttttgccgc
    21301 tatttctctg ttctcgctat tattccaacc gctgtttggt ctgctttctg acaaactcgg
    21361 gctgcgcaaa tacctgctgt ggattattac cggcatgtta gtgatgtttg cgccgttctt
    21421 tatttttatc ttcggaccac tgttacaata caacatttta gtaggatcga ttgttggtgg
    21481 tatttatctt ggcttttgtt ttaacgccgg tgcgccagca gtagaggcat ttatcgagaa
    21541 agtcagccgt cgcagtcatt tcgaatttgg tcgcgcgcgg atgtttggct gtgttggctg
    21601 ggcgctgtgt gcctcgattg tcggcatcat gttcaccatc aataatcagt ttgttttctg
    21661 gctgggttct ggctgtgcac tcatcctcgc cgttttactc tttttcgcca aaacggatgc
    21721 gccctcttca gccacggttg ccaatgcggt aggtgccaac cattcggcat ttagccttaa
    21781 gctggcgctg gaactgttca gacagccaaa actgtggttt ttgtcactgt atgttattgg
    21841 cgtttcctgc acctacgatg tttttgacca acagtttgct aatttcttta cttcgttttt
    21901 tgctaccggt gaacagggta cgcgggtatt tggctacgta acgacaatgg gcgaattact
    21961 taatgcctcg attatgttct ttgcgccact gatcattaat cgcatcggtg ggaaaaatgc
    22021 cctgctgctg gctggcacta ttatgtctgt acgtattatt ggctcatcgt tcgccacctc
    22081 agcgctggaa gtagttattc tgaaaacgct gcatatgttt gaagtaccgt tcctgctggt
    22141 gggctgcttt aaatatatta ccagccagtt tgaagtgcgt ttttcagcga cgatttatct
    22201 ggtctgtttc tgcttcttta agcaactggc gatgattttt atgtctgtac tggcgggcaa
    22261 tatgtatgaa agcatcggtt tccagggcgc ttatctggtg ctgggtctgg tggcgctggg
    22321 cttcacctta atttccgtgt tcacgcttag cggccccggc ccgctttctc tactgcgtcg
    22381 tcaggtgaat gaagtcgctt aagcaatcaa tgtcggatgc ggcgcgagcg ccttatccga
    22441 ccaaaatatc agggcggaat gatcgcaatg aacatgtcga tgacagaaag aataaaagca
    22501 ggcaagctat ttaccgatat gtgcgaaggc ttacctgaaa aaagacttcg tggaaaaacg
    22561 ttaatgtatg agtttaatca ctcgcatcca tcagaatttg aaaaaaggga aagtctgatt
    22621 aaagaaatgt ttgccacggt aggggaaaac gcctgggtag aaccccccgt ctatttctct
    22681 tacggttcca acatccatat aggccgcaat ttttatgcaa atttcaattt aaccattgtc
    22741 gacgactaca cggtaacgat cggtgataac gtactgattg cacccaacgt tactctttcc
    22801 gttacgggac acccggtaca ccatgaattg agaaaaaacg gagagatgta ctcttttccg
    22861 ataacgattg gcaataacgt ctggatcgga agtcatgtgg ttattaatcc aggcatcacc
    22921 atcggggata attctgttat tggcgcgggt agtgtcgtca caaaagacat tccaccaaac
    22981 gtcgtggctg ctggcgttcc ttgtcggatt attcgcgaaa taaacgaccg ggataagcaa
    23041 tattatttca aagattttaa agttgagtct tcagtttaaa taagcaaaat tacctggtgc
    23101 gctccgctta tcaggcctac aatttcagtg atctacgtag gccggataag gcgttcacgc
    23161 cgcatccggc ataaactacg ctcgcggtaa cccagccgat taccgtttgt aatcgatggc
    23221 ttctggctgc tccagatata cggtggtttg tgccggtttt gtgctggcaa tcaccttgcc
    23281 gccacgtacc gaatagcgca ccggaacctg acggcgcagc gcgtcaaacc cattttcagc
    23341 cggcaggata atcaggttgg cgctgtttcc ggcggcaatg ccgtaatcct gcaaattcaa
    23401 cgttctggca ctgtggtggg tgattaaatt caggccgtcg ttaatctgcc cgtagcccat
    23461 cagctggcaa acatgcagcc ccatatgcag cacttgcagc atattcgccg ttcccagcgg
    23521 ataccacgga tcgaagacat catcgtgacc aaagcagacg ttaatgccgg actccagcat
    23581 ctctttaacg cgcgtgatgc cgcgacgttt tggatacgta tcgaaacgtc cttgcagatg
    23641 aatattgacc aacgggttgg cgacaaagtt aataccggac attttcagca aacggaacag
    23701 gcgcgaagta tacgccccgt tataggagtg cattgccgta gtatggctgg cggtgactcg
    23761 cgcgcccatg ccttcacggt gcgccagggc agcaacggtt tcgacaaagc gtgattgctc
    23821 atcatcaatc tcgtcacagt gaacatcgat gagacggtcg tatttttgcg ccagggcgaa
    23881 ggttttatgc agcgactcca cgccatattc acgggtaaat tcaaaatgcg gaatcgcccc
    23941 aacaacgtct gcccctaagc gtaacgcctc ttccagcaac gcttcaccat tgggatacga
    24001 caaaatccct tcctgcggga aggcgacgat ttgtagatca atccacggcg caacttcctg
    24061 tttcacttcc agcattgctt tcagcgcagt cagcgttgcg tccgaaacat cgacatgggt
    24121 acgcacatgc tgaatgccgt tggcaatctg ccatttcagc gtttgccatg cgcgttgttt
    24181 cacatcgtca tgagttaata acgctttgcg ctcggcccag cgttcaatgc cttcaaacag
    24241 cgtgccggac tgattccagt tcggttgtcc ggcggtttgc gtggtgtcca ggtgaatatg
    24301 cggctccaca aacggcggta aaactaaacc ttgttcggca tccaggctgt tttcagttac
    24361 gggcatcacg ccggattgcg catcaatggc gctgattttt ccgtcgtgaa aatgaatctg
    24421 ccacagcccc tctttgccgg gtaaccgggc gttaataatt gtttgtaaag cattattcga
    24481 cactgttagc ctccacatgc gtcattgctg ctgtcgtttt acgattcaaa atcgggttaa
    24541 ggatcagata gctcagcgcg ccacctaata ccgcgttgac cggaacaatt cccggtaacc
    24601 agtggcctgc ggcaatcccc agcgcgacag ccagaatcgc cacccaattg acactcatca
    24661 tacgcgtggt cgcaaagtgc tcatagcggc gacggttcat cagataatca gcgatgatca
    24721 cgccgcccac cggaggaata gctgccgaaa ggaaggtcaa ccagccgaca aaattgttat
    24781 acagccataa tgcgcagacc gtaccgataa taccgttaat caccgaaagg gttttgctcg
    24841 acataccggt aatgttggcg aaacctaaac ccgacgcata gagcgcgtta tcgttggtgg
    24901 tccagatatt cagccccagc accacaatcg caggcagcag caggccctga gcaatcatca
    24961 catcagagat atccgccatc cccagtgccg cagcgcccgc tgcaccgaaa ataaacatca
    25021 acgagttgcc gaggaaaaag gccaccatcg ccaccagcac cgccagtttg gcattacgac
    25081 caaaccggac aaagtcagcg gtgagcgtgc ccgcactgat aaatgacccc acaaccagcg
    25141 ccagcgcgac attgaaatct aacggttgtg cgggaacgac cgcttttaat gcgtccaggc
    25201 cgcccatgcc gttaacggcc aaccacacgg aataaccgcc aagacaggcg atagccggaa
    25261 ccgcaatcac cgaaagaact gtcagcgccg aaatgccaaa gaagacggtg acggtcatca
    25321 gtaaaccgga gacggcaatc agcaaattaa tatccagccc ggttgccttg cccaccggaa
    25381 tagcaaacat cgccacgcca acgccaaacc agccaacctg ggtgccgccc agcagcagtg
    25441 aaggcaacca tgagccttta acaccaaacg agaagcgagc aagaagatgg gtggtcaggc
    25501 cggtttttgc gccaatgtaa ccaagaaatg aagtgtaaat accgaggaga agattaccga
    25561 tgagaactgc gaggaagaaa tcatgatagc taagaccggt tccgagagtg ccgccggtcc
    25621 acatactggc ggaaaagaag gttaatccca gcatgacgaa cgtcaatgcc aatacccctt
    25681 tccgcgccga ctgcgggact ggcccctggc taaagttgtt atcttgcgac acgaaattcc
    25741 ccctttttgc tgtttgaaac ccaaaaaatc cgccgcattc tattcatctg aagataaaaa
    25801 gcaatcgttt tcgtatgggg atatatttct tatatgaatg agtacaaaaa ggcgggggaa
    25861 acttatctgt gtaaaaacag tgggtaggca tgataagacg cgtcagcgtc gcatcaggca
    25921 tctgcgcatg gtgtcggatg cggcgcgagc gccttatccg acctacggtt caggcctgta
    25981 ggcatgataa gacgcgtcag cgtcgcatca ggcatctgcg cacggtgtcg gatgcggcgc
    26041 gagcgcctta tccgacctac tcttccatcg cctggcggat ctgatccaac gacgccggat
    26101 catcaatggt cgtcagatcc cccggatcgc gtccttcgca aatcgcctgg atcgtgcggc
    26161 gcagcatttt tccggatcgc gtttttggca attgcgagac aaaccagacg tgcgccggac
    26221 ggccaaagtt gccaatctgg ctgtccacca gcgccataat cgccttctct tgcgagtgcg
    26281 ccacctcacg gtcttccaga ctgtcgctct ctttcggaat gacaaacgcc accgccacct
    26341 gccctttcag cgcatctttc accccaacca ccgccacttc ggcaacgccc ggatgactgg
    26401 agatactctc ttcaatctca cgcgtaccca gccgatgtcc ggcaacgtta atcacatcat
    26461 cagtacgccc caggataaag tgataaccgt cagcatcgcg gatgccccag tcaaaagtgg
    26521 cgtacaccgg acgggaaaac agcgaccagt acgtcttcac aaagcggtcg tcgtcgcccc
    26581 agatggtctg aatacagcct ggcggcaatg gcccctccac caccagcatc cctttctcat
    26641 tgacgccaca cggttcgccg gtgacttcat tgagcaactg cacgttatag ccatacatcg
    26701 gcacgccggg gcttcccaga cgcgtcggcc tgtcatccag accgcgagca atcgccataa
    26761 tcggccagcc ggattcggtc tgccagtaat tgtcgatgac cggcacatcc agcgtattgc
    26821 tcacccaact ggcggtcggc tcgtccagcg gttctccagc cagatagagc acttccagcg
    26881 acgagagatc gtgtttgcga atttcagcgg tagggaattt tttcagcacg cgaatggcgg
    26941 tcggcgctga gaacatccgg ctaacctgat atttctcgac gattttccac cacacgccgc
    27001 agtccggcca ggtcggtaat ccttcgtaaa cgatagtcgc catccccgcc agcagcggcg
    27061 cgtagacgat atacgaatgc cccaccaccc agccgatatc cgaagcacaa aagaacacac
    27121 cgcccgcttt gccgccaaaa atggtttcca tcgaggtcgc cagcgccacc gcatatccgc
    27181 cgacgtcacg ctgcacgcct ttaggtttgc cggtcgtgcc ggaagtgtag agaatgcagg
    27241 aggtttcgtt ggattccagc cacgccatcg gtacccgcgc gccgatgtgt tgatggcgca
    27301 acgacgcgaa atcgacatcc cgcccgctga cgcgtgccat tttcgccagc ccgcgatcca
    27361 ccagtaaaac gtggcgcggc tgatgctgtg cctgacttat cgcatcgtcg agcaattttt
    27421 tatacggaat gattttaccg ccgcgcgccc cggcatcagc cgagacaatc agcaccggtt
    27481 tagcgtcatc aattcgcgcc gccacgctgt gcgaggcaaa tccaccaaac accaccgagt
    27541 gaatagcacc aatgcgcgcg caggccagca gggtaatatg cgcttcggca atcatcggca
    27601 tatacaccag cacccgatcg ccacgctgca cgcccagcga gcgcagcatt gacgccaccg
    27661 cattcacttc gtcatgcagt tggcggaagg taaaggtacg ctcttcctct gtttccgaag
    27721 agacggcaat caatgccagc gcctctggct gtttctccag ccagcgatcg atagcgttgt
    27781 gacacaagtt ggttcggcct tcacaaaacc aacgggcaaa cggcgggttg ctgtggtcga
    27841 gcgtttgcgt aaagggcgtc tgccagtcaa tacgccgggc ctgctcggcc cagaactgct
    27901 ccggttcgtt aatcgaacgc tgataaaatt cgctaaaaga cataatgctc tcctgttgaa
    27961 cttacgcctt accgccgttt acttaaatga cgtacaggtc gagatactca ttgaccggca
    28021 tctgttccag gcgagttctg tcgagagaaa cctccagaat gcgctgctgc tggcgagtcg
    28081 ggaactggcg cgcgagattg attttgaatt tatcgaccag cttcggaatg ccatcctgac
    28141 ggcggcgagc atgaccaatg gggtactcca ccaccacttc ttcaaagcgc gtgccgtcgg
    28201 tgaactcaag ggttatggca ttggcgatgg cgcgtttttc cgggtcgtgg tagtcagcgg
    28261 taaacgccgg atcttcaaag caattgatct tctcgcgcag ggcgtcgatg cgtttatctt
    28321 gcgcaacgtt gtcttcgtaa tctgccgccg ttaagcgccc gaacagcagc gggatcgcca
    28381 ccatgtactg aatgcagtgg tcgcggtctg ccgggttatt gagcggccct tttttgtcga
    28441 tgatgcgaat acaggcttcg tgggtgcgaa tggtcacttt ttcgatatcc gccgccgttt
    28501 tgcctgctgc ctgcatctgt tcatagagcg tcatcgccgc ttcaactgct gtctgggagt
    28561 ggaactccgc cgggaaggag attttgaaca gcacattttc cataacgtaa gaaccgtatg
    28621 ggcgctggaa gcggaacgac tcacctttaa aggagacgtc gtagaagccc cacaccggcg
    28681 cggtcagagc tgacgggtaa cccatttcgc cggttttcgc catcagtgcc agacgtaccg
    28741 cgcgggaagt ggcatcgccc gccgcccagg atttacgcgt gccggtattc ggcgcatggc
    28801 gataggtgcg cagcgactga ccatctaccc acgccagcga aacggcgttg agaatttcct
    28861 cgcgggtcag gccgagcatt tcggcgacca cggcggtgga agccactttc actaacagaa
    28921 cgtggtcgag accaacgcgg ttaaaggagt tttccagcgc aatgcagccc tgaatttcat
    28981 gggctttgat cattccggtc agcacctgtt tcatggtcaa cggcgctttg ccgctggcga
    29041 ccgcgttgcg cgaaagccag tccgccgttg ccagaatgcc gccgaggttg tcggaaggat
    29101 gcccccactc cgccgccagc caggtatcgt tgaaatcgag ccaacggatc atcgcgccaa
    29161 tgttaaatgc cgcctggacg gggtcgaact gaaactgagt tccggggacg cgcacgccat
    29221 tgggtacgac ggtgccgggg acaattggcc ccagcagttt tttacaggcc ggatattcga
    29281 gagcttccag accgcagccg agcgtgtcga gcaggcagta atgtgcggtg tcgtaggcta
    29341 ctctggagct gatttcgtag ttcatcacgt aatcgacgat atcaacgatt tcacgatcaa
    29401 attccgggcg gatgttgttg atttgagctg acattgggta cgtttccttc ttgttattcg
    29461 tagaggttta ctggcgctta tccagcgcga caaactggcg gtcttccggt ccaacgtaat
    29521 tggcggaagg acggataatt ttgttgtcct gacgttgttc gataatgtgc gccgcccagc
    29581 cagtgacgcg agcgataaca aacagtggtg tgaacatctc ggtgggaaca cccatcatgt
    29641 tgtaggaaac agcggagaac cagtcgagat tggggaacat ctttttgctc tcccacatca
    29701 ctgtttccag gcgatcggcg atgttgtaca tcttcagcga gccgccttcc tgcgagagct
    29761 gctttgccac gcgtttgatc acctggtggc gcgggtcggc gatggtataa accggatgac
    29821 caaaaccaat gaccacttct ttgttttcca cccgcttacg gatatcagct tcggcttcgt
    29881 ccggcgtttc gtagcgttgc tggatctcca gcgacacttc attcgccccg ccgtgtttcg
    29941 gcccgcgcag tgcgccaatc gcgccaataa tggcggaata catatcagag ccagtgcccg
    30001 caatcacccg gctggtaaag gtggaagcgt taaactcgtg ttcggcgtac aacaccagcg
    30061 agatatgcat cgccttttcc cagctttgcg acggcttttc gccgtgcagc agatgcagga
    30121 agtgaccgcc gatagagtcg tcatcagttt ccggctggat gcgttcgccg ttgtggctgt
    30181 agtgatacca gtagagaaga atcgaactaa gtgacgccag cagtttgtcg gcaatatccc
    30241 gcgcgccaga aacggtgtgc ccctcttttt ctggcagcgt gcagccgagc gcggaaacgc
    30301 cggtgcgcat aacatccatc gggtgtgacg ccgccggtaa ggcttccagc acggtacgca
    30361 cgttagccgg taaaccacgc agggctttca gtttcgtttt gtaggcggcg agttcgtcac
    30421 gggttggcag tttgccgtgg atcagcaggt gcgccacttc ttcaaattca caatgttccg
    30481 ccagatcaag aatatcgtag ccacggtaat gcaggtcgtt gccgctttta cccacggtgc
    30541 agagcgccgt attgcccgcc ggaacgccgg aaagtgccac cgattttttc ggtttaatga
    30601 catgggtact gttttgcagg atcgttgtgt cgctcatgtt gtcctcgtca ttgttatcat
    30661 ttgtagggtc gagaatgttg tcggatgcga cgccatgcgt cttatccgac ctacagttca
    30721 atgcatcccg taggcctgat aagtcgcgtc agcggcgcat caggcatcgg ttgtcgaata
    30781 caaccaacgg gcgtttttat ttcacctgac cacgggcaaa caggtcgtcg agcttctctt
    30841 cgtactggta gtagttgatg ctttcgtaca gctcgttgcg ggtctgcatg gtgtcgatga
    30901 cgcttttctg tgtgccttcc tgacgcagga tgttgtagac atgttcagcg gcgcggttca
    30961 tggcgcgaaa cgctgaaagc gggtagagcg ccattgcgac atgggcgctg cgtaattcgt
    31021 cggtggtaaa cagcggtgtt gcgccaaatt cggtaatgtt ggcgaggatc ggcacctgca
    31081 ccgcatcggc aaactggcga tacatggcga gttcggtaat cgcctccggg aacagcattt
    31141 cggcacccgc ttcaacatag gcctgcgcac gctcgatcgc cgcatccagc ccctctaccg
    31201 ccagcgcatc ggtgcgcgcc atgatcacaa aatcaggatc ggttttcgca tccaccgccg
    31261 cgcggatccg atccaccatc tcttctttcg agacgatcgc tttattcgga cgatgaccgc
    31321 agcgtttcgc accaacctga tcttcaatat gcaatcctgc cgcaccggct ttaatcatcg
    31381 atttcaccgt acgcgccacg ttaaaggctg aagaaccaaa accgatatcc gcatccacca
    31441 gcagcggcag cgaacaaacg tcggtaatac ggcgaatgtc agtcagcaca tcatcaagag
    31501 tagaaatacc gagatcgggc agccccagcg atcctgccgc cacgccaccg ccggagagat
    31561 aaatcgtctg atatccggca cgctgcgcca gcagcgcatg gttagcgttg atggtgccaa
    31621 caatttgcaa cggggtttct ttgctaagtg cagcgcgaaa cgctttacct ggagagtgta
    31681 gaggcatagc ccatcctttg ttatcaactt gttatttacg ttgataaaga caaagcaatg
    31741 ggtgtgccac aattgcctta ataaaattaa acatttaatt tcaattagtt aaagataaag
    31801 tcatgaagta atccgcaaat atgcgtttca gttaacgttt caggcaatgt ttcaccgcgt
    31861 ttcattgcaa caattatgaa acaagactaa acccaatatt cggtttctta actttgcggt
    31921 gcgctatggc acatccacca cggctgaatg acgacaaacc ggttatctgg acggtatctg
    31981 taacgcgcct gttcgagctg tttcgcgata tcagcctcga gtttgatcac ctggcgaata
    32041 tcacccctat tcagcttggc tttgaaaaag cggtgaccta catccgcaag aaactggcaa
    32101 acgaacgctg cgatgccatc atcgctgctg gctctaacgg cgcatatctt aaaagccgcc
    32161 tgtcagtgcc agttattttg attaaaccga gcggctacga tgtattacag gcactggcaa
    32221 aagccggaaa actcacctct tctatcggcg ttgtcactta tcaggaaact attccggcac
    32281 tggtggcgtt tcaaaaacct ttaatttgcg cctcgaccaa cgtagctaca ttaccgaaga
    32341 agacgcgcgc gggcagatta acgagctgaa agctaacggc accgaagcgg tggtcggcgc
    32401 ggggctgatt accgatctgg cagaagaagc cggaatgacc ggaattttta tctattccgc
    32461 cgccaccgtg cgccaggcat tcagcgatgc gctggatatg acgcgcatgt cgttacgcca
    32521 taacactcac gatgccaccc gcaatgccct gcgtactcgt tacgtgctgg gcgatatgct
    32581 cggtcaatca ccacagatgg aacaggtgcg gcagactatt ttgctgtatg cccgctccag
    32641 tgcggcggtg ttgattgagg gggaaacggg gacgggcaaa gagctggcgg cccaggcgat
    32701 tcatcgggaa tattttgccc gccacgatgc gcgacagggc aaaaagtcgc atccgtttgt
    32761 tgccgtcaac tgcggggcga ttgccgaatc gctgctggaa gcagagctgt ttggctatga
    32821 ggaaggggcg tttaccggct cgcgacgcgg aggacgcgcc ggactgtttg aaatcgccca
    32881 cggtggcacg ctgtttcttg acgagatagg cgaaatgccg ctgccgttgc agactcggct
    32941 gctgcgggtg ctggaagaaa aagaggtcac ccgcgtcggc gggcatcagc ctgttccggt
    33001 ggatgtgcgg gtcattagcg ccactcactg caatctggaa gaagatatgc agcaagggca
    33061 gtttcgccgt gacctgtttt atcggctgag tattttgcgt ctgcaactgc cgccgctacg
    33121 cgagcgggtg gcggatattc tgccgctggc ggaaagcttt ctgaaagtgt ctctggcggc
    33181 gctctccgcc ccgttttctg ccgcattacg ccaggggtta caggcaagcg aaaccatgct
    33241 gttgcgctac gactggccgg gcaatattcg tgaactgcgc aatatgatgg aacgactggc
    33301 actattttta agtgtggaac cgacgccgga tttaacgccg caatttttgc agctgctact
    33361 gccggaactg gcgcgcgagt cggcgaagac tcccattcca ggcttgctga cagcacaaca
    33421 ggcactggag aaatttaatg gcgataaaac agcagcggcg aattatttag gtatcagccg
    33481 gacgacgttc tggcggcggc tgaaaagctg aactgccgaa tgcagcgcga acgctttatc
    33541 cggcataaat ttcgatagat gatgatcacc gataaactaa cgcatcgggg atgtttacat
    33601 aggattcaga attacttctt cttataaata tttgccgtgc cgtggatctt attgtcagtt
    33661 tgaccagagg tcagtaccaa cacatcagcc cctttttcat cggctttttt gatcaaatct
    33721 tctttcgcat ctgcggtcga catttcatta ctggttgaaa tatcacctat tttttcatat
    33781 tgcgatgcaa ccttttcaaa ttccgctttg gtcattaatt ctgcggcata gacattggta
    33841 acggcaaacg ctaacgcacc gactaacatt ttagagatta ttttcatatt acggctccag
    33901 agttgttatc acactatcac ccacccacat tattgggttg attaataatt agttcagcca
    33961 gggcataaac gccagtaacc ggaaataaga cactttgtga tctctctcgc acccgattat
    34021 ttttggtaga aatatgatga agttacttca cttatttatg gatgaaatca ctatggatcc
    34081 tttgcacgcc gtttacctga ccgtaggatt gttcgtgatt acttttttta atccgggagc
    34141 caatctcttt gtggtagtac aaaccagcct ggcttccggt cgacgcgcgg gggtgttgac
    34201 cgggctgggc gtggcgctgg gcgatgcatt ttattccggg ttgggtttgt ttggtcttgc
    34261 aacgctaatt acgcagtgtg aggagatttt ttcgcttatc agaatcgtcg gcggcgctta
    34321 tctcttatgg tttgcgtggt gcagcatgcg ccgccagtca acaccgcaaa tgagcacact
    34381 acaacaaccg attagtgccc cctggtatat ctttttccgc cgtggattaa ttaccgatct
    34441 ctctaacccg caaaccgt
//
LOCUS       DAHHTG010000042        34089 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.042, whole genome shotgun sequence.
ACCESSION   DAHHTG010000042 DAHHTG010000000
VERSION     DAHHTG010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 34089)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34089)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34089
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.042"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            complement(<1..884)
                     /locus_tag="NBI74_001970"
     CDS             complement(<1..884)
                     /locus_tag="NBI74_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001566491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCD1932314.1"
                     /translation="MFISDETLRVIHLVARHQSITTAAEQLNKVPSAISYTIKKAEES
                     LGVELFIRKGRYIELSPAGSYFIEHSKTILGDLEALKRNTVLIHDGIERELTIAVNNI
                     IPGDLLVAFIRDFERQFTSTTLTVDLEVYNGCWDALYSKRADLVYGAPHAVPSSEGII
                     SEPVGQMEWDFVVSPLHPLAAKRHPLENSELRHYPAVCIRDTSVNFPPMQAWLLEGQK
                     PIFVPDFATAIALIEQNVGIGYIPHHLALPLLNSGKLLKKPMREHKHATKLFLAARSD
                     GMGKACQWCIEYLRNQQL"
     gene            complement(962..2185)
                     /locus_tag="NBI74_001971"
     CDS             complement(962..2185)
                     /locus_tag="NBI74_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020234945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine permease"
                     /protein_id="HCD1932315.1"
                     /translation="MRKKEENLNTASGLRIAMILLGIAVTPVLLSSSSLGNQLSSGSL
                     ISVVLLGGVILTLLSAITISVGEKARLPTYGIVKYSFGEKGAIAINILMAISLFGWIA
                     VTANMFGHSVHDLLAQHGLEVPLALLVAAGCVIFVASTAFGFAVLGKIAQVAVPVIAL
                     VLCYILYVATHTEVAVPAAIVEMNTGVAVSTVVGTIIVLVATLPDFGSFVHNRKHALI
                     AAGVTFLVAYPLLYWAGATPSAISGQGSLLGAMAVFGAVLPAALLLIFACVTGNAGNM
                     FQGTLVVSTLLTRFPKWQITVALGILSAIVGSMDIMAWFIPFLLFLGIATPPVAGIYI
                     ADFFLYRRNGYQESVLAQESQIKVLTFAAWIIGAAVGFMTVKGLFTLTTIPSVDSILV
                     ACIAYAILSRASQHR"
     gene            complement(2211..2600)
                     /locus_tag="NBI74_001972"
     CDS             complement(2211..2600)
                     /locus_tag="NBI74_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000214032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA amidotransferase"
                     /protein_id="HCD1932316.1"
                     /translation="MTTTLLAVNGTLMRGLELNPNMQKAGGIFVREDRTDAHYRLWSI
                     NDRHPGMIRVNEGGTHVDVEIWQLPLASFAALLMSEPAGLAIGKIKLADGSEVLGVLA
                     ENWLTEGQREITELGSWRKYTGHFHTV"
     gene            complement(2617..3573)
                     /gene="arcC"
                     /locus_tag="NBI74_001973"
     CDS             complement(2617..3573)
                     /gene="arcC"
                     /locus_tag="NBI74_001973"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001606533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HCD1932317.1"
                     /translation="MVKPLAVVAVGGNALIQDEQRNSIPDQYVAVMESVQHIVDMVEA
                     GWDLVLTHGNGPQVGFILRRSELASNEVSPVPLDYAVGDTQGAIGYMFQKALHNELAR
                     RGINKPVIALVTQTRVSPHDDAFASPSKPIGAFLDEATAQQRQQQLGWTLMEDAGRGW
                     RRTVPSPAPLEIIEHDTIAHLVRQGYLVIACGGGGIPVVRDGQQLKGVEAVIDKDLAS
                     ALLASQLGADLLVIPTGVEKVAINFGTPQQQWLDAISVAEAQTLLREGQFGVGSMQPK
                     VEAIVDFINASQQQGKQASGLITSPQTIKAALAHQSGTWITL"
     gene            complement(3566..4990)
                     /locus_tag="NBI74_001974"
     CDS             complement(3566..4990)
                     /locus_tag="NBI74_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001093935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HCD1932318.1"
                     /translation="MNTLFNQPLKVVNAGLHSFADNIQHAGGSAIALNWQPPAQGDID
                     AGLDLASLLRHPLVENANQIAMARYLEAQPVLVDVMLAKEAIPAMAEQKRILHSGPPI
                     AWEEMCGPVKGAIIGAMLYEGWATSQQDAENQINAGEIDLAPCHHYHAVGPMAGIISP
                     SMPLWVVENKTNGHRTFSNFNEGLGKVLRFGANNDEVLNRLAWMRDELTPAMKAAIAQ
                     HGELELKPLMAQALHMGDEVHNRNAAATGLLIKRLLPALLACSLPQEHIQRVVAFITG
                     NDHFFLNLSMAACKAMMDAAANVPFSSMVTVMARNGVNFGIRISGTGDRWFQAPANAV
                     EGLFFPGFGVDDAAADLGDSAITETAGVGGFAMASSPAIVKFVGGTPADATNNSRRMQ
                     AITLGGNPAFTLPALNFAPTAAGIDARKVTDRGILPVINTGIAHKQAGVGQIGAGITT
                     APMACFVAAVRALAEIVAKENHHG"
     gene            complement(4987..6546)
                     /gene="fdrA"
                     /locus_tag="NBI74_001975"
     CDS             complement(4987..6546)
                     /gene="fdrA"
                     /locus_tag="NBI74_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000865643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase FdrA"
                     /protein_id="HCD1932319.1"
                     /translation="MKVKYKVFSNLYQDSVSLMQISAQISKLPGIQQASVVMGTPNNL
                     EQLRDAGLGNEINASPNDLVIAVMGEEDICNEALILAQQRLTSKPDDETDSGIKTPEK
                     VSLEMALEAEPEANLALISVPGDYAAAEAIKALNLGMNVMMFSDNVSIVQEKSIKTLA
                     RERQRIVMGPDCGTAIVNGIPLGFANVVKRGAIGVIGASGTGLQEVTCRIDQLGAGIS
                     QALGTGGHDLSKEIGGISMLFALDALAQDDETRVIVLISKPPSPIVARTILERAEACG
                     KPVVVNFLGANPHDLARPNITAATTLASAADIAVALLNAQPLPAVETDVPCDDLTMLQ
                     NACQRLPAHRQAIRGVFAGGTFCYEAQLICQQKGFSAASNTPVAGNRALANIWQSEDH
                     TLIDMGDDDFTRGKPHPMIDPTLRNQRLLNELNDSHTAVVLFDLVLGYGASTTPASEL
                     LDQLSHIDMNNAPLLIAHVCGTEADPQIRSQQISALQNAGVIIASSNAQAALWASTVA
                     QTQLQKKGLNA"
     gene            complement(6641..7501)
                     /locus_tag="NBI74_001976"
     CDS             complement(6641..7501)
                     /locus_tag="NBI74_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001374637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
                     /protein_id="HCD1932320.1"
                     /translation="MVQVTLCASSLGYLFPQDETQDLRLHSAFKHAVNLYSDAGTFIT
                     LLCAQTYLNLPDAARVWLPECWDWRREIAHSDPIQLTPGLLRTPRFCVALDNATLWQS
                     PFVGGMLTLEAFPLVFQHYPTMASQRLLFCLEHNVQSTLHLPDSLTHQGLAIMEHPDA
                     LERQVPQLIGFGKGLTPDGDDYLLGYLAALWLWQLPAPLADHQYRLQQAIDQHAHNTT
                     DISRHYLERALQGHFSEPICQLLAQLVGSASAMTIASCAEQVMQFGATSGVDCLAGML
                     HGFRTLNTMN"
     gene            complement(7507..8175)
                     /locus_tag="NBI74_001977"
     CDS             complement(7507..8175)
                     /locus_tag="NBI74_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HCD1932321.1"
                     /translation="MTQSIFQAQPFELPFDPRTTALVMIDMQRDFVEAGGFGEALGND
                     VSRVRTAIAPCTEVLAAARQKGIMVIHTREGHRADLSDCPPAKLTRGGKTFIGEPGPM
                     GRILVRGEAGHDIIPELYPVAGEPVIDKPGKGAFYQTDLHLILQNHGIKTLIVCGVTT
                     EVCVTTTVREANDRGYECIIPEDCVGSYFPEFQKYALEMIKAQGAIFGWVTDSKAIIA
                     GLEG"
     gene            complement(8432..9241)
                     /locus_tag="NBI74_001978"
     CDS             complement(8432..9241)
                     /locus_tag="NBI74_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HCD1932322.1"
                     /translation="MATTQQSGFAPAASPLASTIVQTPDDAIVAGFTSIPSQGDNMPA
                     YHARPKQSDGPLPVVIVVQEIFGVHEHIRDICRRLALEGYLAIAPELYFREGDPNDFA
                     DIPTLLGGLVAKVPDSQVLADLDHVASWASRNGGDVHRLMITGFCWGGRITWLYAAHN
                     PQLKAAVAWYGKLTGDKSLNSPKQPVDIATDLNAPVLGLYGGQDNSIPQESVETMRQA
                     LRAANAKAEIIVYPDAEHAFNADYRPSYHAESAKDGWQRMLEWFTQYGVKK"
     gene            9503..10267
                     /gene="udp"
                     /locus_tag="NBI74_001979"
     CDS             9503..10267
                     /gene="udp"
                     /locus_tag="NBI74_001979"
                     /EC_number="2.4.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001593007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine phosphorylase"
                     /protein_id="HCD1932323.1"
                     /translation="MSKSDVFHLGLTKNDLQGATLAIVPGDPDRVEKIAALMDKPVKL
                     ASHREFTTWRAELDGKPVIVCSTGIGGPSTSIAVEELAQLGIRTFLRIGTTGAIQPHI
                     NVGDVLVTTASVRLDGASLHFAPLEFPAVADFECTTALVEAAKSIGATTHVGVTASSD
                     TFYPGQERYDTYSGRVVRHFKGSMEEWQAMGVMNYEMESATLLTMCASQGLRAGMVAG
                     VIVNRTQQEIPNAETMKQTESHAVKIVVEAARRLLK"
     gene            complement(10302..11792)
                     /locus_tag="NBI74_001980"
     CDS             complement(10302..11792)
                     /locus_tag="NBI74_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001606539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HCD1932324.1"
                     /translation="MFESELLTQGFSTLLNNPQALLFATFGVMLGIVIGALPGLTATM
                     GVAILLPFTYGMEPVSGLLMICGVFFGGVYGGSITAILLKIPGTPAAAATAIDGYELT
                     KQGKAGLALSAATFSSFSGGTLSIIVLMFLSPVLASWALKFSASESFALATFGLSIIA
                     SISGESLIKGLIAGVGGLLIATIGLDPMGGFPRFTGGFVELMNVPFIPVMIGLFAASE
                     AFRSMEQNQQIRQGAKVAIGSLLLPWQTLRRIALTILRSSGLGVFIGMIPGAGADIAA
                     FVAYNETRRFSKTPENFGKGEIKAVASCEAGANGCTGGALLPMLTLGIPGDAVTAIML
                     GALTLQGMQPGPLMFTDHGDMVYTLFVGMIFCYFMLLVLGLLSLKVIGNVVKIPGNIL
                     TPMILALCVVGTYALNNSLFDVGIMLIAGVVGYFMQKGGYPASPVVLALIMGPMAESN
                     FRRALSLSGGSLDFLYTRPITLALLTLAAFTLLTPIIRKIMRLRRQ"
     gene            complement(11805..12311)
                     /locus_tag="NBI74_001981"
     CDS             complement(11805..12311)
                     /locus_tag="NBI74_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001596577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HCD1932325.1"
                     /translation="MNTKQSVAQLAVSHRKRLSSTMVVALLLCVVAGAVMINAADFPA
                     TAIETDPGASAFPTFYACALIVLAVLLVIRDLLQAKPASCANAQEKPAFRKTATGIAA
                     TAFYIVAMSYCGYLITTPVFLIVIMTLMGYRRWVLTPGIALLLTAILWLLFVEALQVP
                     LPVGTFFE"
     gene            complement(12328..13302)
                     /locus_tag="NBI74_001982"
     CDS             complement(12328..13302)
                     /locus_tag="NBI74_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HCD1932326.1"
                     /translation="MIKHLTLPKKMAAMVLLAGAALSIAPVQAASYPTKQIELVVPYA
                     AGGGTDLVARAFADAAKNHLPVSIGVINKPGGGGAIGLSEIAAARPGGYKIGLGTVEL
                     TTLPSLGMVRFKTSDFKPIARLNADPAAITVRADAPWNSYEEFMTYAKANPGKVRIGN
                     SGTGAIWHLAAAALEDKTGAKFSHVPYDGAAPAITGLLGGHIEAVSVSPGEVINHVKG
                     GKLKTLVVMADERMKTMPDVPTLKEKGVDLSIGTWRGLIVSQKTPQDVVDVLAKAAKE
                     TAEEPAFQDALQKLNLNYAWLDAASFQTQISEQEKYFDELLTRLGLKK"
     gene            complement(13327..13956)
                     /locus_tag="NBI74_001983"
     CDS             complement(13327..13956)
                     /locus_tag="NBI74_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001116470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-6-phosphogalactonate aldolase"
                     /protein_id="HCD1932327.1"
                     /translation="MPFNTLLQKTGLVAILRGVKPDEIVAIGEKLYAAGFRLIEIPMN
                     SPEALQSISLLRDALPKDCLVGAGTVLSVEQVVAVKEAGGQIIVMPHCDTAVIRRARA
                     LGMYCAPGVATPTEAFTAIEHGANAIKLFPAEQITPEVTKAWRAVIPQSVPMLPVGGI
                     TPETMARYLSHGANGFGLGSALYHPGMTPEQVYENAVLFMNAWNNLNSN"
     gene            complement(13946..14950)
                     /locus_tag="NBI74_001984"
     CDS             complement(13946..14950)
                     /locus_tag="NBI74_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001117527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygalactonokinase"
                     /protein_id="HCD1932328.1"
                     /translation="MPGAPSFIALDWGTSSLRAWRFGESPNPQEKREFPWGIMKLPSQ
                     AATREDAFHDTFLRVCGDWLAQTPCPVLACGMLGSAQGWQPAAYLPCPVTLEGLAKQL
                     TPVIHQQQTMLHIIPGVIKEGEMPEVMRGEETQIFGAISMEPSLQNAIHQGMPVLIGL
                     PGTHAKWAVVENNTITDFRTFMTGELFDVLSRHSILGATMHPGDELHWDAFTHGLTAA
                     QEHHQTGLLSTLFSTRSRLLTSNLTSSSQGDYLSGLLIGHELCGLASSLLRDLPATTP
                     IALIGSANLNSRYSQAFSHVFPDRQIHAIPNATEQGLWRIAHAAGLLSTNARECTHAI
                     "
     gene            complement(14974..15741)
                     /locus_tag="NBI74_001985"
     CDS             complement(14974..15741)
                     /locus_tag="NBI74_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000852399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HCD1932329.1"
                     /translation="MAETSSTTAGAQTLLRGLAVLNAVYNGCHDLKSIGEFTGTTRST
                     THRLVTVLVEQRYLRHVPTQGYQLGAKLIEFGARALESTSLYEIAQPVLQRLARYTLD
                     TVHLGIVEGDEVLYLEKINSQRGLEMRSRPGHRMPLAITGIGKALILNRTEEEWRTLF
                     KTCGDETKLGAFIQNMRRYAASGFAFDLEENEPTIRCVAAPVYNARDEIVAAISVAST
                     TTYMSLARLEELAPYVKSCAEEISAELGWGKHVRKDK"
     gene            15958..17385
                     /gene="rmuC"
                     /locus_tag="NBI74_001986"
     CDS             15958..17385
                     /gene="rmuC"
                     /locus_tag="NBI74_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HCD1932330.1"
                     /translation="MDFSIMVYAVIALVGVAIGWLFASYQHAQQKAEQLAEREEMVAE
                     LSAAKQQITQSEHWRAECELLNNEVRSLQSINTSLEADLREVTTRMEAAQQHADDKIR
                     QMINSEQRLSEQFENLANRIFEHSNRRVDEQNRQSLNSLLSPLREQLDGFRRQVQDSF
                     GKEAQERHTLTHEIRNLQQLNAQMAQEAINLTRALKGDNKTQGNWGEVVLTRVLEASG
                     LREGYEYQTQVSIETDTRSRMQPDVIVRLPQGKDVVIDAKMTLVAYERYFNAEDDYTR
                     ESALQEHIASVRNHIRLLGRKDYQQLPGLRTLDYVLMFIPVEPAFLLALDRQPELITE
                     ALKNNIMLVSPTTLLVALRTIANLWRYEHQSRNAQQIADRASKLYDKMRLFIDDMSAI
                     GQSLDKAQDNYRQAMKKLSSGRGNVLAQAEAFRGLGVEIKREINPDLAEQAVSQDEEY
                     RLRSVPEQPNDEAYQRDDEYNQQSR"
     gene            17480..18235
                     /gene="ubiE"
                     /locus_tag="NBI74_001987"
     CDS             17480..18235
                     /gene="ubiE"
                     /locus_tag="NBI74_001987"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HCD1932331.1"
                     /translation="MVDKSQETTHFGFQTVAKEQKADMVAHVFHSVASKYDVMNDLMS
                     FGIHRLWKRFTIDCSGVRRGQTVLDLAGGTGDLTAKFSRLVGETGKVVLADINESMLK
                     MGREKLRNIGVIGNVEYVQANAEALPFPDNTFDCITISFGLRNVTDKDKALRSMYRVL
                     KPGGRLLVLEFSKPIIEPLSKAYDAYSFHVLPRIGSLVANDADSYRYLAESIRMHPDQ
                     DTLKTMMQDAGFESVDYYNLTAGVVALHRGYKF"
     gene            18249..18854
                     /gene="ubiJ"
                     /locus_tag="NBI74_001988"
     CDS             18249..18854
                     /gene="ubiJ"
                     /locus_tag="NBI74_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis protein UbiJ"
                     /protein_id="HCD1932332.1"
                     /translation="MPFKPLVTAGIESLLNTFLYRSPALKTARSRLLGKVLRVEVKGF
                     STSLILVFSERQVDVLGEWAGDADCTVIAYASVLPKLRDRQQLAALIRSGELEVQGDI
                     QVVQNFVALADLAEFDPAELLAPYTGDIAAEGISKALRGGAKFLHHGIKRQQRYVAEA
                     ITEEWRMAPGPLEVAWFAEETAAVERAVDALTKRLEKLEAK"
     gene            18851..20491
                     /gene="ubiB"
                     /locus_tag="NBI74_001989"
     CDS             18851..20491
                     /gene="ubiB"
                     /locus_tag="NBI74_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis regulatory protein
                     kinase UbiB"
                     /protein_id="HCD1932333.1"
                     /translation="MTPGEVRRLYFIIRTFLSYGLDELIPKMRITLPLRLWRYSLFWM
                     PNRHKDKPLGERLRLALQELGPVWIKFGQMLSTRRDLFPPHIADQLALLQDKVAPFDG
                     KLAKQQIEAAMGGLPVEAWFDDFEIKPLASASIAQVHTARLKSNGKEVVIKVIRPDIL
                     PVIKADLKLIYRLARWVPRLLPDGRRLRPTEVVREYEKTLIDELNLLRESANAIQLRR
                     NFEDSPMLYIPEVYPDYCSEGMMVMERIYGIPVSDVATLEKNGTNMKLLAERGVQVFF
                     TQVFRDSFFHADMHPGNIFVSYEHPENPKYIGIDCGIVGSLNKEDKRYLAENFIAFFN
                     RDYRKVAELHVDSGWVPPDTNVEEFEFAIRTVCEPIFEKPLAEISFGHVLLNLFNTAR
                     RFNMEVQPQLVLLQKTLLYVEGVGRQLYPQLDLWKTAKPFLESWIKDQVGIPALVRAF
                     KEKAPFWVEKMPELPELVYDSLRQGKYLQHSVDKIARELQSNHVRQGQSRYFLGIGAT
                     LVLSGTFLLVSRPEWGLMPGWLMAGGLIAWFVGWRKTR"
     gene            20570..20839
                     /gene="tatA"
                     /locus_tag="NBI74_001990"
     CDS             20570..20839
                     /gene="tatA"
                     /locus_tag="NBI74_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA"
                     /protein_id="HCD1932334.1"
                     /translation="MGGISIWQLLIIAVIVVLLFGTKKLGSIGSDLGASIKGFKKAMS
                     DDEPKQDKTSQDADFTAKTIADKQADTNQEQAKTEDAKRHDKEQV"
     gene            20843..21358
                     /gene="tatB"
                     /locus_tag="NBI74_001991"
     CDS             20843..21358
                     /gene="tatB"
                     /locus_tag="NBI74_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatB"
                     /protein_id="HCD1932335.1"
                     /translation="MFDIGFSELLLVFIIGLVVLGPQRLPVAVKTVAGWIRALRSLAT
                     TVQNELTQELKLQEFQDSLKKVEKASLTNLTPELKASMDELRQAAESMKRSYVANDPE
                     KASDEAHTIHNPMVKDNETVHEGVTPAAAQTQASSPEQKPETTPEPVVKPAADAEPKT
                     AAPSPSSSDKP"
     gene            21361..22137
                     /gene="tatC"
                     /locus_tag="NBI74_001992"
     CDS             21361..22137
                     /gene="tatC"
                     /locus_tag="NBI74_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatC"
                     /protein_id="HCD1932336.1"
                     /translation="MSVEDTQPLITHLIELRKRLLNCIISVIVIFLCLVYFANDIYHL
                     VSAPLIKQLPQGSTMIATDVASPFFTPIKLTFMVSLILSAPVILYQVWAFIAPALYKH
                     ERRLVVPLLVSSSLLFYIGMAFAYFVVFPLAFGFLANTAPEGVQVSTDIASYLSFVMA
                     LFMAFGVSFEVPVAIVLLCWMGITSPEDLRKKRPYVLVGAFVVGMLLTPPDVFSQTLL
                     AIPMYCLFEIGVFFSRFYVGKGRNREEENDAEAESEKTEE"
     gene            22179..22961
                     /gene="tatD"
                     /locus_tag="NBI74_001993"
     CDS             22179..22961
                     /gene="tatD"
                     /locus_tag="NBI74_001993"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' ssDNA/RNA exonuclease TatD"
                     /protein_id="HCD1932337.1"
                     /translation="MFDIGVNLTSSQFAKDRDDVVARAFDAGVNGLLITGTNLRESQQ
                     AQKLARQYSSCWSTAGVHPHDSSQWQAVTEEAIIELAAQPEVVAIGECGLDFNRNFST
                     PEEQERAFVAQLRIAAELNMPVFMHCRDAHERFMTLLEPWLDKLPGAVLHCFTGTREE
                     MQACVACGIYIGITGWVCDERRGLELRELLPLIPAEKLLIETDAPYLLPRDLTPKPSS
                     RRNAPAHLPHILQRIAHWRGEDAAWLAATTDANVKTLFGIAF"
     gene            complement(22958..23446)
                     /gene="rfaH"
                     /locus_tag="NBI74_001994"
     CDS             complement(22958..23446)
                     /gene="rfaH"
                     /locus_tag="NBI74_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription/translation regulatory transformer
                     protein RfaH"
                     /protein_id="HCD1932338.1"
                     /translation="MQSWYLLYCKRGQLQRAQEHLERQAVNCLAPMITLEKIVRGKRT
                     AVSEPLFPNYLFVEFDPEVIHTTTINATRGVSHFVRFGASPAIVPSAVIHQLSVYKPK
                     DIVDPSTPYPGDKVIITEGAFEGFQAIFTEPDGEARSMLLLNLINKEIKHSVKNTEFR
                     KL"
     gene            23613..25106
                     /gene="ubiD"
                     /locus_tag="NBI74_001995"
     CDS             23613..25106
                     /gene="ubiD"
                     /locus_tag="NBI74_001995"
                     /EC_number="4.1.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-polyprenylbenzoate decarboxylase"
                     /protein_id="HCD1932339.1"
                     /translation="MDAMKYNDLRDFLTLLEQQGELKRITLPVDPHLEITEIADRTLR
                     AGGPALLFENPKGYSMPVLCNLFGTPKRVAMGMGQEDVSALREVGKLLAFLKEPEPPK
                     GFRDLFDKLPQFKQVLNMPTKRLRGAPCQQKIVSGDDVDLNRIPIMTCWPEDAAPLIT
                     WGLTVTRGPHKERQNLGIYRQQLIGKNKLIMRWLSHRGGALDYQEWCAAHPGERFPVS
                     VALGADPATILGAVTPVPDTLSEYAFAGLLRGTKTEVVKCISNDLEVPASAEIVLEGY
                     IEQGETAPEGPYGDHTGYYNEVDSFPVFTVTHITQREDAIYHSTYTGRPPDEPAVLGV
                     ALNEVFVPILQKQFPEIVDFYLPPEGCSYRLAVVTIKKQYAGHAKRVMMGVWSFLRQF
                     MYTKFVIVCDDDVNARDWNDVIWAITTRMDPARDTVLVENTPIDYLDFASPVSGLGSK
                     MGLDATNKWPGETQREWGRPIKKDPDVVAHIDAIWDELAIFNNGKSA"
     gene            25152..25853
                     /gene="fre"
                     /locus_tag="NBI74_001996"
     CDS             25152..25853
                     /gene="fre"
                     /locus_tag="NBI74_001996"
                     /EC_number="1.5.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-flavin reductase"
                     /protein_id="HCD1932340.1"
                     /translation="MTTLSCKVTSVEAITDTVYRVRIVPDAAFSFRAGQYLMVVMDER
                     DKRPFSMASTPDEKGFIELHIGASEINLYAKAVMDRILKDHQIVVDIPHGEAWLRDDE
                     ERPMILIAGGTGFSYARSILLTALARNPNRDITIYWGGREEQHLYDLCELEALSLKHP
                     GLQVVPVVEQPEAGWRGRTGTVLTAVLQDHGTLAEHDIYIAGRFEMAKIARDLFCSER
                     NAREDRLFGDAFAFI"
     gene            complement(26034..27197)
                     /gene="fadA"
                     /locus_tag="NBI74_001997"
     CDS             complement(26034..27197)
                     /gene="fadA"
                     /locus_tag="NBI74_001997"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadA"
                     /protein_id="HCD1932341.1"
                     /translation="MEQVVIVDAIRTPMGRSKGGAFRNVRAEDLSAHLMRSLLARNPA
                     LEAAALDDIYWGCVQQTLEQGFNIARNAALLAEVPHSVPAVTVNRLCGSSMQALHDAA
                     RMIMTGDAQACLVGGVEHMGHVPMSHGVDFHPGLSRNVAKAAGMMGLTAEMLARMHGI
                     SREMQDAFAARSHARAWAATQSGAFKNEIIPTGGHDADGVLKQFNYDEVIRPETTVEA
                     LATLRPAFDPVSGTVTAGTSSALSDGAAAMLVMSESRARELGLKPRARVRSMAVVGCD
                     PSIMGYGPVPASKLALKKAGLSASDIGVFEMNEAFAAQILPCIKDLGLMEQIDEKINL
                     NGGAIALGHPLGCSGARISTTLLNLMEHKDVQFGLATMCIGLGQGIATVFERV"
     gene            complement(27207..29396)
                     /gene="fadB"
                     /locus_tag="NBI74_001998"
     CDS             complement(27207..29396)
                     /gene="fadB"
                     /locus_tag="NBI74_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadB"
                     /protein_id="HCD1932342.1"
                     /translation="MLYKGDTLYLDWLEDGIAELVFDAPGSVNKLDTATVASLGEAIG
                     VLEQQSDLKGLLLRSNKAAFIVGADITEFLSLFLVPEEQLSQWLHFANSVFNRLEDLP
                     VPTIAAVNGYALGGGCECVLATDYRLATPDLRIGLPETKLGIMPGFGGSVRMPRMLGA
                     DSALEIIAAGKDVGADQALKIGLVDGVVKAEKLIEGAMAILRQAINGDLDWKAKRQPK
                     LEPLKLSKIEATMSFTIAKGMVAQTAGKHYPAPITAVKTIEAAARFGREEALNLENKS
                     FVPLAHTNEARALVGIFLNDQYVKGKAKKLTKDVETPKQAAVLGAGIMGGGIAYQSAW
                     KGVPVVMKDINDKSLTLGMTEAAKLLNKQLERGKIDGLKLAGVISTIHPTLDYAGFDR
                     VDVVVEAVVENPKVKKAVLAETEQKVRPDTVLASNTSTIPISELANALERPENFCGMH
                     FFNPVHRMPLVEIIRGEKSSDETIAKVVAWASKMGKTPIVVNDCPGFFVNRVLFPYFA
                     GFSQLLRDGADFRKIDKVMEKQFGWPMGPAYLLDVVGIDTAHHAQAVMAAGFPQRMQK
                     DYRDAIDALFDANRFGQKNGLGFWRYKEDSKGKPKKEEDVVVDDLLAKVSQPKRDFSE
                     EEIIARMMIPMVNEVVRCLEEGIIATPAEADMALVYGLGFPPFHGGAFRWLDTLGSAK
                     YLDMAQQYQHLGPLYEVPEGLRNKARHNEPYYPPVEPARPVGDLKTA"
     gene            29586..30917
                     /gene="pepQ"
                     /locus_tag="NBI74_001999"
     CDS             29586..30917
                     /gene="pepQ"
                     /locus_tag="NBI74_001999"
                     /EC_number="3.4.13.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro dipeptidase"
                     /protein_id="HCD1932343.1"
                     /translation="MESLASLYKNHIATLQERTRDALARFKLDALLIHSGELFNVFLD
                     DHPYPFKVNPQFKAWVPVTQVPNCWLLVDGVNKPKLWFYLPVDYWHNVEPLPTSFWTE
                     DVEVIALPKADGIGSLLPAARGNIGYIGPVPERALQLGIEASNINPKGVIDYLHYYRS
                     FKTEYELACMREAQKMAVNGHRAAEEAFRSGMSEFDINIAYLTATGHRDTDVPYSNIV
                     ALNEHAAVLHYTKLDHQAPEEMRSFLLDAGAEYNGYAADLTRTWSAKSDNDYAQLVKD
                     VNDEQLALIATMKAGVSYVDYHIQFHQRIAKLLRKHQIITDMSEEAMVENDLTGPFMP
                     HGIGHPLGLQVHDVAGFMQDDSGTHLAAPAKYPYLRCTRILQPGMVLTIEPGIYFIES
                     LLAPWREGQFSKHFNWQKIEALKPFGGIRIEDNVVIHENNVENMTRDLKLA"
     gene            30917..31531
                     /locus_tag="NBI74_002000"
     CDS             30917..31531
                     /locus_tag="NBI74_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMPACT family protein"
                     /protein_id="HCD1932344.1"
                     /translation="MESWLIPAAPVTVVEEIKKSRFITLLAHTDGVEAAKAFVESVRA
                     EHPDARHHCVAWVAGAPDDSQQLGFSDDGEPAGTAGKPMLAQLMGSGVGEITAVVVRY
                     YGGILLGTGGLVKAYGGGVNQALRQLTTQRKTPLTEYTLQCEYSQLTGIEALLGQCDG
                     KIINSDYQAFVLLRVALPAAKVAEFSAKLADFSRGSLQLLAIEE"
     gene            31570..33021
                     /gene="trkH"
                     /locus_tag="NBI74_002001"
     CDS             31570..33021
                     /gene="trkH"
                     /locus_tag="NBI74_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkH"
                     /protein_id="HCD1932345.1"
                     /translation="MHFRAITRIVGLLVILFSGTMIIPGLVALIYRDGAGRAFTQTFF
                     VALAIGSMLWWPNRKEKGELKSREGFLIVVLFWTVLGSVGALPFIFSESPNLTITDAF
                     FESFSGLTTTGATTLVGLDSLPHAILFYRQMLQWFGGMGIIVLAVAILPILGVGGMQL
                     YRAEMPGPLKDNKMRPRIAETAKTLWLIYVLLTVACALALWFAGMDAFDAIGHSFATI
                     AIGGFSTHDASIGYFDSPTINTIIAIFLLISGCNYGLHFSLLSGRSLKVYWRDPEFRM
                     FIGVQFSLVVICTLVLWFHNVYSSALMTINQAFFQVVSMATTAGFTTDSIARWPLFLP
                     VLLLCSAFIGGCAGSTGGGLKVIRILLLFKQGNRELKRLVHPNAVYSIKLGNRALPER
                     ILEAVWGFFSAYALVFIVSMLAIIATGVDDFSAFASVVATLNNLGPGLGVVADNFTSM
                     NPVAKWILIANMLFGRLEVFTLLVLFTPTFWRE"
     gene            33033..33578
                     /gene="hemG"
                     /locus_tag="NBI74_002002"
     CDS             33033..33578
                     /gene="hemG"
                     /locus_tag="NBI74_002002"
                     /EC_number="1.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone-dependent protoporphyrinogen IX
                     dehydrogenase"
                     /protein_id="HCD1932346.1"
                     /translation="MKTLILFSTRDGQTREIASYLASELKELGIQADVANVHRIEEPQ
                     WENYDRVVIGASIRYGHYHSAFQEFVKKHATRLNSMPSAFYSVNLVARKPEKRTPQTN
                     SYARKFLMNSQWRPDRCAVIAGALRYPRYRWYDRFMIKLIMKMSGGETDTRKEVVYTD
                     WEQVANFAREIAHLTDKPTLK"
     gene            33954..>34089
                     /locus_tag="NBI74_002003"
     rRNA            33954..>34089
                     /locus_tag="NBI74_002003"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT         8099 a         8729 c         9075 g         8186 t
ORIGIN      
        1 attaattgct ggtttcttaa atattctatg caccactgac aggctttgcc cataccatcg
       61 gaacgagcgg caagaaataa cttggtggcg tgtttatgtt cgcgcattgg ctttttaagc
      121 aatttaccgc tgttcaataa cggtaatgcc agatgatgtg gaatataacc aataccgacg
      181 ttttgttcta ttaaggcgat tgccgtagcg aagtcaggaa caaaaatggg tttttgtcct
      241 tccaacaacc aggcttgcat cggggggaag ttaacggagg tatcgcggat acaaaccgct
      301 ggataatgac gcaattcact gttttctaat ggatgccgct tcgctgccag tgggtggagt
      361 gggctgacga caaaatccca ttccatttgt ccgaccggtt cgctaatgat gccttcactg
      421 cttggtactg catgaggagc accgtagacc agatcagcac gcttactgta cagtgcatcc
      481 cagcagccgt tgtatacctc aagatctacc gtcagtgttg ttgatgtgaa ttgccgttca
      541 aaatctcgaa taaatgccac cagcaaatca ccaggaataa tattgtttac cgcaattgtc
      601 agttcacgct caataccatc gtgaatcagc actgtgttgc gctttaatgc ttccaggtcg
      661 ccaagaatag ttttgctatg ttcgatgaag taacttcccg ctggcgataa ttcaatatag
      721 cgtcccttac gaatgaacag ttcaacgccc aggctctctt ccgctttctt gatggtgtaa
      781 ctgatggcgg aagggacttt atttaactgc tcggctgcgg tggtaatgct ctggtgtctg
      841 gcgaccagat gaataacacg aagcgtttcg tctgagatga acataaggct ccctggtcag
      901 tggtaaagtg gctgaatctc tccagattca gccgtaatca ttgcctgagt atacccagga
      961 attagcggtg ttgacttgcc cgactgagaa tcgcatacgc gatacatgcc accagaatcg
     1021 agtctaccga agggatcgtc gtcagggtga ataagccttt tacggtcata aagccaaccg
     1081 ctgcgcctat gatccatgct gcgaatgtca gcactttaat ctgtgactcc tgggctaaca
     1141 ctgactcttg atagccatta cggcgataaa ggaaaaagtc agcgatataa attccggcaa
     1201 cgggtggcgt ggcgataccc aggaacagca gaaacggaat aaaccacgcc ataatatcca
     1261 tactgcctac gatggcggaa aggataccca gcgccacggt aatctgccat ttgggaaagc
     1321 gggtaagcag tgtggaaacc accagcgtgc cctggaacat attgcccgcg ttaccggtga
     1381 cgcaggcgaa aatcaacaac agcgccgcag gcagaactgc accgaatacc gccatcgcac
     1441 ccagtaaaga tccctgacca ctaatggcgc tcggcgttgc acccgcccag tagagcagag
     1501 ggtaggcaac cagaaacgtc acgcctgcgg caatcagcgc atgtttgcgg ttatgcacaa
     1561 aactaccgaa atcaggcagt gtggcaacca gcacaataat ggtgccaaca acggtggaaa
     1621 cggcgacacc tgtattcatc tccacaatcg ccgctggtac tgccacttcg gtatgggttg
     1681 ccacatagag gatgtaacac agcaccagcg cgataaccgg cacggcaacc tgggcaattt
     1741 ttcccagaac ggcaaagcca aatgccgtag aggcgacaaa aatgacacag ccagccgcca
     1801 ctaacagtgc cagtggaact tccagtccat gttgagccag taagtcatgt accgaatgac
     1861 caaacatatt ggcggtaacg gcaatccagc cgaacagact tatcgccatc aaaatgttaa
     1921 tggcgatggc ccctttttcg ccaaacgaat atttcacaat gccatacgtt ggcaggcggg
     1981 ctttttctcc cacgctaatg gtgatggctg aaagtaaggt cagaatgacg ccgcctaaca
     2041 atacgacgct aattaaactg ccgctggaaa gttgattgcc gaggcttgag gatgacaaca
     2101 gcacaggtgt gacggcaata cccagcaaaa tcatggcaat acgcaatcct gatgccgtat
     2161 tcagattctc ttctttttta cgcatagtat gttcccttta taaatgcgcg ttacacagta
     2221 tgaaaatgcc cggtgtattt acgccagcta cccagttctg taatttcccg ttggccttcg
     2281 gtcagccagt tttctgccag tacgcccagc acttcgctac catcggcaag tttgattttg
     2341 ccaattgcca gcccagcggg ttcgctcatt aacagagcgg caaaactggc cagcggtaat
     2401 tgccaaattt ctacgtcgac gtgtgttcct ccttcattca cgcgaatcat tcccggatgg
     2461 cgatcattaa tactccacaa ccggtaatgg gcgtcagttc ggtcttcacg gacaaaaata
     2521 ccgcccgctt tttgcatatt tgggttgagt tccaggccgc gcatcagcgt tccgttaacg
     2581 gctaaaagcg ttgtagtcat tgtgttctcc agagaattaa agggttatcc atgtaccgct
     2641 ctgatgcgcc agggctgctt ttatggtttg cggtgaagta atcaggccgc tggcctgttt
     2701 gccttgttgc tggctggcat tgatgaaatc aacaatggct tccactttgg gttgcatact
     2761 gccgacacca aactgacctt cccgcaacag cgtttgcgct tcggcaacgc tgatagcgtc
     2821 gagccactgt tgttgtggtg taccaaagtt aatcgccact ttttctacac cggtggggat
     2881 caccagcaga tctgcgccta actgactggc gagcagcgcg gaggccagat ctttatcgat
     2941 cacggcttct acacctttca gttgttgccc gtctcgcacc accggaatac cgccgccgcc
     3001 gcaggcaata accagatatc cctggcgcac caggtgagcg atggtgtcgt gctcaataat
     3061 ttccagtggt gcaggagagg gaactgtacg ccgccaacca cgcccggcgt cctccatcag
     3121 cgtccagccg agttgttgtt ggcgttgttg ggccgttgct tcatcgagaa acgcgccaat
     3181 cggtttactg gggctggcga aagcatcgtc atgtgggctg actcgcgttt gtgtcaccag
     3241 ggcaattacc ggtttgttta tgccacggcg agcgagttcg ttatgcagcg ctttctggaa
     3301 catgtagcca attgcacctt gtgtatcacc cacggcgtaa tcaagtggaa ccggagaaac
     3361 ttcgttactg gcgagttcag agcggcgcag aataaagccc acctgcgggc cattaccgtg
     3421 ggttagcacc aggtcccatc cggcttcaac catatcaacg atatgctgca cgctttccat
     3481 cactgcaaca tattgatcgg gaatactatt gcgttgctcg tcctgaatga gcgcattgcc
     3541 gccaaccgcg acgacagcca gtggcttaac catgatggtt ctcctttgcg acgatctccg
     3601 ccagtgcacg tacagcggcg acaaaacagg ccatcggcgc agtggtgatc ccggcaccaa
     3661 tttgcccgac ccccgcttgt ttgtgggcga tacccgtatt gatgaccggt aaaatgccgc
     3721 gatcggtcac tttacgcgca tcaatccctg ctgcggtggg ggcaaaattg agggctggaa
     3781 gtgtaaatgc ggggttaccg ccgagggtga tggcttgcat ccgacggctg ttattggtgg
     3841 catcggcagg ggtgcctccc acaaatttga cgatcgccgg agacgacgcc atcgcgaagc
     3901 cacccacacc tgcggtttca gtaattgcgc tatcacccag gtcagcagca gcgtcgtcaa
     3961 cgccaaatcc ggggaagaac agaccttcaa cggcattggc aggagcctgg aaccagcgat
     4021 cgccagtacc ggaaatccga ataccaaaat tcaccccgtt acgcgccatc accgtaacca
     4081 ttgaactgaa cggtacattg gcggcggcat ccatcattgc tttacaggcg gccatcgaca
     4141 gattgaggaa gaagtgatcg ttaccggtga taaatgccac gacgcgctgg atatgttcct
     4201 gtggaagaga gcaggccagc aatgccggga gcaggcgctt aatcagcaaa ccggtagcgg
     4261 cagcattgcg gttatgaact tcatccccca tatgcagcgc ctgcgccatt aatggcttga
     4321 gttccagctc gccgtgttgt gcgattgctg ctttcattgc cggcgtcagt tcatcgcgca
     4381 tccaggccag acgattgaga acttcatcgt tattggcgcc aaaacggaga accttaccca
     4441 gaccttcatt gaaattgcta aaagtacggt ggccgttggt tttgttttct accacccata
     4501 acggcatcga cgggctgatg atgcctgcca ttgggccaac cgcgtgataa tgatgacaag
     4561 gggcaaggtc gatttcgcct gcgtttatct ggttttcagc gtcttgctgg ctggtggccc
     4621 aaccttcgta cagcatggca ccaataatcg ccccttttac cggaccacac atctcttccc
     4681 aggcaatcgg cgggccagag tgcaaaatac gtttttgttc agccattgct ggaatggcct
     4741 ctttagcaag catgacatcc accagcacgg gctgtgcttc gagataacgt gccatggcga
     4801 tttggttggc attttctacc aggggatgac gtagcaacga tgccagatcc aatccagcgt
     4861 caatatcgcc ttgtgctggt ggctgccagt tgagtgcaat tgcgcttccc ccggcatgtt
     4921 gaatattgtc agcaaaactg tgtagtcccg cgttcacaac cttcaaaggc tgattaaata
     4981 aggtattcat gcgtttagcc cctttttctg caactgagtc tgtgcaacgg tgctggccca
     5041 tagcgccgcc tgtgcattgc tgctggcgat gatgacccct gcattttgta gtgcgcttat
     5101 ctgctgactg cgtatttgcg gatcagcttc cgttccgcaa acgtgcgcaa tcagcaacgg
     5161 agcgttattc atgtcgatgt gtgaaagttg gtccagtaat tcgctggcag gcgtcgtgga
     5221 agcgccataa ccaagtacca ggtcgaataa cacgacggca gtgtgtgagt cgttgagttc
     5281 gttgagcaga cgttgattgc gcagagtcgg gtcgatcatt ggatgcggtt tgccacgggt
     5341 gaaatcatcg tcgcccatat cgatcaacgt gtgatcttcg ctttgccaga tatttgccag
     5401 agcacgatta cccgcaactg gcgtattgga agctgcgctg aatccctttt gttggcagat
     5461 aagttgcgct tcgtagcaga aggttcctcc ggcgaaaaca ccgcgtatcg cctggcgatg
     5521 ggctggcagg cgctggcagg cgttttgtaa cattgtgaga tcgtcgcagg gtacatcggt
     5581 ttcgacagcc ggaagtggct gagcattcag taacgccaca gcgatgtcgg cggcgctggc
     5641 aagtgtggtg gcggcagtga tattgggacg tgccagatca tgcggattcg ccccgaggaa
     5701 attcaccacc acgggttttc cacaagcctc ggcgcgctcc agaatagtcc gggcaacgat
     5761 cggtgaaggc ggttttgaaa tcagcacaat tacgcgagtt tcatcgtctt gcgccagcgc
     5821 atctaaagcg aagagcatcg atattccacc aatctcttta ctgagatcat gtccgcctgt
     5881 tcccaacgcc tgagaaatac ccgctccgag ctggtcgatt cggcaagtca cttcctgtag
     5941 cccggtgcct gacgcgccaa taacaccaat tgctccgcgt ttcaccacat tagcgaagcc
     6001 taacggaatg ccgttaacaa tggcggttcc acagtcaggt cccattacga tgcgctgacg
     6061 ttctcgggca agcgttttga tggatttttc ctgcacgata ctgacgttat cgctgaacat
     6121 catcacgttc attccgaggt ttaacgcttt aattgcttcg gcggcggcgt agtctcccgg
     6181 tacagaaatg agtgccaggt tggcttcggg ttcggcttcc agtgccattt ccaggctgac
     6241 cttttccggt gttttaatgc cactatccgt ttcatcatcg ggtttgctgg tcagacgttg
     6301 ctgggccagt atcagtgctt cattgcaaat atcttcttca cccatgactg caatcactaa
     6361 atcattggga ctggcgttga tttcgttacc cagacccgca tcgcgcaatt gttcgagatt
     6421 attgggcgtt cccatgacga ccgaggcctg ttgaataccg ggcaacttgc tgatttgcgc
     6481 ggaaatctgc atcagagaaa ctgagtcctg ataaagatta ctgaaaactt tatatttaac
     6541 tttcatgctg ttacgcctgt ttcattccgc ccgatagcaa aattcgggca aagcatcccc
     6601 gtgtagcccc ataaacgggc tacgggagaa aaggttgaaa tcaattcatg gtgttcaggg
     6661 ttcgaaagcc atgcagcatt cctgcaaggc aatctactcc tgacgttgcg ccaaattgca
     6721 tgacctgttc tgcacaggat gcgattgtca ttgccgatgc gctcccaacc agttgtgcga
     6781 gtaactggca aatcggttct gaaaaatgtc cctgaagcgc acgctccaga taatgacgac
     6841 tgatatccgt ggtgttgtgc gcatgttgat caattgcctg ctgcaaccgg tattgatgat
     6901 cggcaagtgg tgcagggagt tgccataacc agagcgctgc cagatagccc aacaaatagt
     6961 catctccatc gggcgttaag cctttaccaa agccgatcag ttgtggcacc tggcgttcca
     7021 gcgcatctgg atgctccata atcgctaatc cctggtgcgt gagactatcc ggcaggtgca
     7081 atgtgctttg aacgttatgc tccagacaaa ataaaagccg ttgcgatgcc atcgttgggt
     7141 agtgctggaa aaccaaaggg aatgcctcaa gtgtcagcat tccccccaca aatggcgact
     7201 gccagagcgt tgcgttatcg agcgccacac aaaatcgagg tgtccgcagc aagcctgggg
     7261 ttaactgaat gggatctgaa tgggcaatct ctcttcgcca gtcccaacat tcaggtagcc
     7321 agactctggc agcatcaggg agattcagat acgtttgcgc acacaacaga gtgatgaatg
     7381 ttcccgcgtc tgaatacaga tttaccgcat gtttaaaagc gctatgtaga cgtaaatctt
     7441 gcgtttcatc ctgcgggaaa agatatccca acgacgatgc gcaaagcgta acctgtacca
     7501 tcggcgttag ccttccagac ctgcaataat ggctttggaa tcagtaaccc aaccgaaaat
     7561 ggcaccttgt gctttgatca tctcgagggc gtatttctgg aattccggga aataggagcc
     7621 gacacaatct tccgggataa tgcattcata gccacgatca tttgcttcgc gcacggtggt
     7681 ggttacgcaa acttcggttg ttacaccaca aactatcagc gtcttgatac cgtggttttg
     7741 caaaatcaga tgtaagtcgg tttgataaaa cgcgcctttg ccaggcttat caatcaccgg
     7801 ttcgcctgcc accgggtaca gttcaggaat gatgtcatga cctgcttcac ctctgaccag
     7861 aatgcgtccc atcggtcctg gttcaccaat aaaggttttt ccgccgcgcg tcagtttggc
     7921 tggcgggcag tcgcttaagt ctgcacgatg gccttcacga gtatgaataa ccataattcc
     7981 tttttgacgt gcggctgcca gcacctctgt acatggtgcg atagccgtgc gtacccgtga
     8041 aacatcattg cccagtgcct caccgaagcc cccggcttcg acaaagtcac gctgcatgtc
     8101 gatcatgacc agtgcagttg tgcgcggatc aaaaggtaat tcaaagggtt gagcctgaaa
     8161 aatggattgc gtcatggtaa atctcctgtc tgatgtttaa acgatccagt tgttctgaga
     8221 acgagtattc aggagaaaag cggcacaaaa caggaaaaaa gtttgccgca cagcaacgaa
     8281 atttttgcat tcagcaggtg gtgtgatgac gaatgtgaag ggagtcaaag agtggcatac
     8341 agcaacaaat tttttgatgc tgtatggttt tgagattaaa aaaatcgttg aaattgtgtg
     8401 agcttaatca tcagatttag cgactatggc gttacttttt aacaccatac tgtgtaaacc
     8461 attccaacat tcgctgccag ccgtctttcg cagactcggc atgatagctc ggacgataat
     8521 cggcgttgaa cgcatgctct gcatccgggt acacgataat ctctgctttc gcgtttgctg
     8581 cccgcagcgc ctggcgcatc gtttccacac tctcttgcgg aatgctgtta tcctggccgc
     8641 catataagcc gagaaccggc gcgttaagat cggttgcgat atcaacaggt tgtttcggtg
     8701 aattcagcga cttatcgccc gtcagtttgc cgtaccacgc cactgcggct tttagctgtg
     8761 gattatgcgc ggcatacagc caggtgatac gtccacccca gcagaatccg gtgatcatta
     8821 aacgatgaac atcgccgccg ttgcgggaag cccaactggc gacatgatcg agatcggcca
     8881 gcacctgcga gtcaggcact tttgctacca gaccgccaag caacgtgggg atatcggcaa
     8941 aatcattcgg atcgccttcg cggaagtaaa gttcaggtgc gatagccaga tacccctcca
     9001 gcgccagacg gcgacaaatg tcgcggatat gttcatgcac gccaaaaatt tcctgcacta
     9061 caatgaccac tggcagtggg ccatcgcttt gctttggtct ggcatggtaa gcaggcatgt
     9121 tatccccttg tgaagggata gaggtgaagc ccgccacaat cgcgtcgtcc ggagtctgaa
     9181 cgatggtcga agcgagaggc gatgcagcag gtgcaaatcc agattgttgt gttgttgcca
     9241 tggtattctc cgtaccctta taaaaatgtt gcgcaatgtt aactatagtc agcatgcaac
     9301 aaatcacatt gcctgaatcg gctcatcttt tatgcagtcc tgcagaatga agagtgattt
     9361 atgtgatttg catcactttt gatgggtaaa tttatgcaac acattttcgt catggtgatg
     9421 agtatcacga aaaaatgtta aacccttcgg taaagtgtct ttttgcttct tctgactaaa
     9481 ccgattcaca gaggagttgt atatgtccaa gtctgatgtt tttcatctcg gcctcactaa
     9541 aaacgattta caaggggcta cgcttgccat cgtccctggc gacccggatc gtgtggaaaa
     9601 gatcgccgcg ctgatggata agccggttaa gctggcatct caccgcgaat tcaccacctg
     9661 gcgtgcagag ctggatggta aacctgttat cgtctgctct accggtatcg gcggcccgtc
     9721 tacctctatt gctgttgaag agctggcaca gctgggcatt cgcaccttcc tgcgtatcgg
     9781 tacaacgggc gctattcagc cgcatattaa tgtgggtgat gtcctggtta ccacggcgtc
     9841 tgttcgtctg gatggcgcga gcctgcactt cgcaccgctg gaattcccgg ctgtcgctga
     9901 tttcgaatgt acgactgcgc tggttgaagc tgcgaaatcc attggcgcga cgactcacgt
     9961 tggcgtgaca gcttcttctg ataccttcta cccaggtcag gaacgttacg atacttactc
    10021 tggtcgcgta gttcgtcact ttaaaggttc tatggaagag tggcaggcga tgggcgtaat
    10081 gaactatgaa atggaatctg caaccctgct gaccatgtgt gcaagtcagg gcctgcgtgc
    10141 cggtatggta gcgggggtta tcgttaaccg cacccagcaa gagatcccga atgctgagac
    10201 gatgaaacaa accgaaagcc atgcggtgaa aatcgtggtg gaagcggcgc gtcgtctgct
    10261 gaaataaaaa aacgctcccc aacttcgggg agcgatatca gttattgacg ccgtaaacgc
    10321 attattttgc ggattattgg cgtcagtagc gtaaaggctg ccagagtcag caatgccaga
    10381 gtgatcggtc gggtatacag aaagtcgagt gacccaccag aaagcgacag cgcacggcga
    10441 aaattacttt ccgccattgg ccccataatc aatgccagca ctacgggtga tgccggatat
    10501 cctcctttct gcatgaaata gcccaccacg cctgcaatca gcataatgcc aacatcaaaa
    10561 aggctattgt tcaacgcata agtcccgacc acacaaagtg cgaggatcat cggtgtgaga
    10621 atattgccgg gaattttcac cacattaccg atgactttca aagagagcag tccaagaact
    10681 agtagcatga agtaacagaa gatcatgccg acaaacagtg tataaaccat atcgccgtgg
    10741 tcggtaaaca tcagcgggcc tggttgcatt ccctgtaacg ttaacgcgcc cagcatgatg
    10801 gcggttaccg catcgccagg gatccccagc gtcagcatag gtaacaatgc gccaccggtg
    10861 cagccattcg cgcctgcctc acaggaggcc acagctttaa tttcaccctt accaaagttt
    10921 tctggtgtct tactgaaacg acgagtttcg ttataggcaa caaaagctgc aatatccgcc
    10981 cccgcaccgg ggatcatgcc gataaaaacc cctaatcctg atgagcgcaa aatggttaat
    11041 gcgatgcggc gtagtgtttg ccagggcagt aacagactgc cgatagccac cttcgccccc
    11101 tgacgaattt gctggttttg ctccatagag cggaatgctt ctgaagcagc aaataaacca
    11161 atcatcaccg ggataaatgg cacattcatc agctccacaa atccacctgt aaaccgtgga
    11221 aaaccgccca ttggatcaag gcctatcgtt gcgatcagca atccgccaac cccggcaatc
    11281 agccccttaa tcagcgactc accggaaata ctggcaataa tgctcagtcc gaaggttgcc
    11341 agggcgaagg actccgaggc actaaatttc agcgcccaac tggcaagtac cggagagaga
    11401 aacatcagca cgataatgct gagcgttccg ccactaaagg aagagaacgt ggcggcactc
    11461 aatgccagcc ctgcttttcc ctgcttcgtc agctcatatc cgtcaatggc ggtggctgcg
    11521 gcggcaggcg taccgggaat tttgagcaaa attgcggtga tagaaccgcc atagacgccg
    11581 ccaaaaaaga cgccgcaaat catcaacaag ccagaaacag gctccatgcc gtaggtgaaa
    11641 ggcagcaaaa tcgccacacc catggtcgct gtcagaccag gcagagcgcc aatcacaata
    11701 cccagcatca ccccaaaagt ggcaaacagc agcgcctgag gattattgag taatgtcgaa
    11761 aaaccctgag ttaaaagttc actttcaaac attgctggcc cctgttattc gaaaaatgtg
    11821 ccgacaggca atggcacctg taacgcttcg acaaacagca accagaggat tgccgttaac
    11881 agcagcgcaa tacccggtgt gagtacccat cgcctgtagc ccatcaacgt cataatgacg
    11941 atgaggaaaa caggagtagt aatgagataa ccgcagtagc tcatcgccac aatataaaac
    12001 gcggttgccg caattcctgt tgccgttttc ctgaatgccg gtttttcctg tgcgttggcg
    12061 caagaggctg gttttgcctg caaaagatcg cgtatcacca gcaagacagc gagcacaatc
    12121 agggcacagg catagaaggt agggaaggca cttgcaccgg gatccgtttc aatggcagtt
    12181 gctggaaaat cagcggcatt aatcatcacc gcgccagcaa ccacacaaag taacagcgcc
    12241 accaccatcg ttgatgaaag gcgcttgcgg tgcgatacgg cgagttgagc aacagactgt
    12301 tttgtgttca tcaaccgtac ctcaggttta ttttttcagg cccaggcgag tcagcaactc
    12361 gtcaaagtac ttttcctgtt cgctgatttg ggtctggaag ctggcagcgt caagccatgc
    12421 atagttgaga ttcaactttt gtagtgcatc ctggaatgca ggctcttcag ccgtctcttt
    12481 tgctgccttt gccagaacat ccaccacatc ctgcggcgtt ttttgcgaca caatcaggcc
    12541 gcgccaggtg ccgatggaga gatcaacgcc tttctctttt aacgtcggga cgtcaggcat
    12601 ggttttcatt cgctcatccg ccatcactac cagtgtcttc agcttgccgc ctttcacatg
    12661 gttgataact tctcctgggc ttacggaaac cgcttcaatg tgcccgccta acaggcctgt
    12721 aatggcaggg gctgcgccgt catacgggac atgagaaaac tttgcgcccg ttttgtcttc
    12781 cagtgcagct gccgccagat gccagatagc tccggtgcct gagttaccaa tgcgtacttt
    12841 tccgggattc gctttggcgt aagtcataaa ttcttcatag ctattccacg gcgcatcggc
    12901 acggactgtg atagcagccg gatccgcatt cagacgggca atgggtttaa agtcgctggt
    12961 tttaaaacgc accattccga ggctgggaag ggtggtcagt tcaaccgtgc ctaaaccaat
    13021 tttgtaaccg ccagggcggg cagcggcgat ttcactcagg ccgatagcac cgcctccgcc
    13081 aggtttattg ataaccccga tgctgacggg taaatggttt ttggcggcat cagcaaaggc
    13141 acgggcaacc agatccgtac cgcctccggc agcgtaggga acgactaact cgatctgttt
    13201 ggttggatag gaggcagcct gtacaggcgc aatgctgagg gcagcgcctg caagaagtac
    13261 cattgcagcc atttttttcg gtaaggtaag atgttttatc attgctttta tatccttatt
    13321 tatcagttag ttgctattta aattgttcca tgcgttcata aacagtactg cattttcata
    13381 gacttgctct ggcgtcatgc cggggtgata tagcgcagaa ccgaggccaa agccgtttgc
    13441 gccgtggctc agataacgcg ccatggtttc cggtgtgata ccgccaaccg gtaacatcgg
    13501 aacggattgt ggaataaccg cacgccaggc tttggtaact tctggcgtaa tttgttcagc
    13561 cgggaacagc ttgatggcat tagcaccatg ttcaatggca gtaaaggctt cggtgggcgt
    13621 ggcgacgcca ggcgcacaat acatacccag cgcacgggcg cgacggatca ccgcagtatc
    13681 gcaatgcggc atcacgataa tctggccccc agcctcttta accgcaacca cctgctctac
    13741 cgaaagcaca gttccggctc cgaccaggca gtccttaggc agggcgtcgc gcagtaaact
    13801 gatactttgc aacgcctccg gcgagttcat aggaatctca atcaggcgga atccggcggc
    13861 gtacagtttt tcaccgatgg cgacaatctc atccggtttc acgccgcgta aaatggcgac
    13921 taacccggtt ttttgcaaaa gcgtattaaa tggcatgggt acattccctg gcgttggtgg
    13981 acagtaaccc ggcagcgtgg gcgattcgcc ataatccctg ttcagtggca ttaggaatgg
    14041 cgtgtatctg tctgtcggga aaaacgtggc tgaatgcttg tgaatagcgg ctgttcagat
    14101 ttgcgctacc gattaaggcg atcggtgttg tggcgggtaa atcacgtagc aaactggatg
    14161 ccagaccaca taattcatgg cctatcagta atccggaaag gtaatccccc tgcgaggacg
    14221 acgtaagatt actggtcagc aggcgcgaac gggtcgaaaa cagcgtcgat aataatccgg
    14281 tctgatgatg ctcttgtgct gctgtcagcc cgtgggtgaa ggcatcccag tgcagttcat
    14341 ctccaggatg catggtggca ccgagaatcg aatggcggga taaaacatca aataactcgc
    14401 ccgtcataaa ggttcggaaa tcggtaatgg tgttgttttc aactactgcc catttcgcat
    14461 gtgtgccggg taagcctatc agcactggca taccctgatg gatcgcattt tgcagggacg
    14521 gttccatcga gatagcaccg aagatttgtg tctcttcgcc acgcatcact tcgggcattt
    14581 caccttcttt aatcacgcca ggaatgatgt gcagcattgt ctgctgctgg tggataacag
    14641 gcgtcaactg cttcgccaga ccctcgagag tgaccgggca aggtaaataa gctgctggtt
    14701 gccacccttg cgcactgccg agcatgccgc aagccagtac cgggcatggg gtttgtgcca
    14761 gccagtcgcc gcagacacgt aaaaacgtat cgtggaaagc gtcctcacgt gttgctgcct
    14821 gggagggtaa tttcatgatc ccccagggga attcacgttt ttcttgtgga tttggtgatt
    14881 cgccaaaacg ccatgcccga agcgatgacg ttccccagtc cagggcaata aatgagggtg
    14941 ctcccggcat tagtcgtttc ctttaatcat ggtttattta tctttgcgga cgtgcttacc
    15001 ccagcccagt tcagcggata tctcttctgc acaggatttg acgtaaggtg ccagttcttc
    15061 cagacgcgcc agcgacatat acgtggtggt actggcgaca gaaatagccg ccacaatttc
    15121 atcccgggcg ttgtaaaccg gcgctgcaac gcaacggata gtcggctcgt tctcttccag
    15181 atcaaaagca aaaccactgg cggcataacg acgcatgttc tgaataaatg cgcccagttt
    15241 tgtttcatca ccacaggtct taaacagcgt gcgccactct tcttctgttc ggttgaggat
    15301 caatgccttg ccgatgccgg tgattgccag tggcatgcga tggcctgggc gtgagcgcat
    15361 ttccagcccg cgctgactgt tgatcttctc cagatacagc acttcgtcgc cttcaacgat
    15421 ccccagatgc acggtgtcca gggtgtaacg ggcaaggcgt tgcaaaacgg gctgggcaat
    15481 ttcgtacagc gaggtgcttt ccagtgctct ggcaccgaat tcgatcagtt tggcacccag
    15541 ttggtagccc tgcgttggga catggcgcag gtagcgttgt tccaccagaa cggtgactaa
    15601 acgatgggtt gtgctccggg tcgtgccggt gaactcgccg atacttttta ggtcatggca
    15661 gccgttataa acagcattaa gaaccgccag tccacgtaat aacgtttgtg cgcctgcggt
    15721 ggttgaagag gtttctgcca tacgtttctc ctgatgatat tgctgaagtg ttccacataa
    15781 tgacgtgggg aaaaggtgat atatcccaca atataaactt catctcatat tatgggataa
    15841 aataatgaaa attaacgatc gttttgcgta gcgcctcgca ggaaacttct ttcaaactgg
    15901 acgtttgtac agcacaattc tattttgtgc gggtaagttg ttgcgtcagg aggcgttgtg
    15961 gatttctcaa tcatggttta cgcagtcatt gcgttggtgg gtgtggcaat tggctggctg
    16021 tttgccagtt atcaacatgc gcagcaaaaa gccgagcaat tagctgaacg tgaagagatg
    16081 gtcgcggagt taagcgcggc aaaacaacaa attacccaaa gcgagcactg gcgcgcagag
    16141 tgtgaattgc tcaataacga agtgcgcagc ctgcaaagta ttaacacctc gctggaggcc
    16201 gatctgcgtg aagtgaccac gcggatggaa gccgcacagc aacacgctga cgataaaatt
    16261 cgccagatga tcaacagcga acagcgcctc agtgagcaat ttgaaaacct tgccaaccgt
    16321 atttttgaac acagtaatcg tcgggttgat gagcaaaatc gccagagtct gaacagtctt
    16381 ttgtcgccgt tgcgtgaaca actggacggt tttcgccgtc aggttcagga cagtttcggt
    16441 aaagaagcac aagaacgtca taccctgact cacgaaatcc gcaatctcca gcaactcaac
    16501 gcgcaaatgg cccaggaagc gatcaacctg acgcgtgcgc tgaaaggcga caataaaacc
    16561 cagggcaact ggggcgaagt ggtgctaacc cgcgtactgg aggcgtctgg tctgcgtgaa
    16621 gggtatgaat atcagactca agtgagtatc gaaaccgaca ctcgttcgcg aatgcaaccg
    16681 gatgtcatcg tgcggctgcc gcagggcaaa gatgtggtga tcgacgccaa aatgacgctg
    16741 gttgcctatg aacgttattt caacgctgaa gatgactaca cacgtgaaag cgcgctacag
    16801 gaacatatcg cgtcggtgcg taaccatatc cgtttgctgg gacgcaaaga ttatcaacag
    16861 ctgccggggc tgcgaactct ggattacgtg ctgatgttta ttcccgttga acccgctttt
    16921 ttactggcgc ttgaccgtca gccggagctg atcaccgaag cgttgaaaaa caatatcatg
    16981 ctggttagcc cgactacgct gctggtggcg ctgcgcacta tcgccaacct gtggcgttat
    17041 gagcatcaaa gccgcaacgc ccagcaaatc gccgatcgtg ccagcaagct gtacgacaag
    17101 atgcgtttgt tcatcgatga catgtccgcg attggtcaaa gtctcgacaa agcgcaggat
    17161 aactatcgcc aggcaatgaa aaaactctct tcaggtcgcg gaaatgtgct ggcgcaggca
    17221 gaagcgtttc gcggtttagg ggtagaaatt aaacgcgaga ttaatccgga tttggctgaa
    17281 caggcggtga gccaggatga agagtatcga cttcggtcgg ttccggagca gccgaatgat
    17341 gaagcttatc aacgcgatga tgaatataat cagcagtcgc gctagcccat tgggggtagt
    17401 taagccgggt agaaatctag ggcatcgacg cccaatctgt tacacttctg gaacaatttt
    17461 ttgatgagca ggcattgaga tggtggataa gtcacaagaa acgacgcact ttggttttca
    17521 gaccgtcgcg aaggaacaaa aagcggatat ggtcgcccac gttttccatt ccgtggcatc
    17581 aaaatacgat gtcatgaatg atttgatgtc attcggtatt catcgtttgt ggaagcgatt
    17641 cacgattgat tgcagcggcg tacgccgtgg gcagaccgtg ctggatctgg ctggtggcac
    17701 cggcgacctg acagcgaaat tctctcgcct ggtcggagaa actggcaaag tggtccttgc
    17761 tgatatcaat gaatccatgc tcaaaatggg ccgcgagaag ctgcgtaata tcggtgtgat
    17821 tggcaacgtt gagtatgttc aggcgaacgc tgaggcgctg ccgttcccgg ataacacctt
    17881 tgattgcatc accatttcgt ttggtctgcg taacgtcacc gacaaagata aagcactgcg
    17941 ttcaatgtat cgcgtgctga aacccggcgg ccgcctgctg gtgcttgagt tctcgaagcc
    18001 aattatcgag ccgctgagca aagcctatga tgcatactcc ttccatgtgc tgccgcgtat
    18061 tggctcactg gtcgcgaacg acgccgacag ctaccgttat ctggcagaat ccatccgtat
    18121 gcatcccgat caggataccc tgaaaaccat gatgcaggat gccggattcg aaagtgttga
    18181 ctactacaat ctgacggcag gggttgtggc gctgcatcgt ggttataagt tctgacagga
    18241 gaccggaaat gccttttaaa cctttagtga cggcaggaat tgaaagtctg ctcaacacct
    18301 tcctgtatcg ctcacccgcg ctgaaaacgg cccgctcgcg tctgttgggc aaagtgctgc
    18361 gcgtggaggt aaaaggcttt tcgacgtcat tgattctggt gttcagcgaa cgccaggtcg
    18421 atgtactggg cgaatgggca ggcgatgctg actgcaccgt tatcgcttac gccagcgtgt
    18481 tgccgaaact tcgcgatcgc cagcagcttg ccgcactgat tcgtagtggt gagctggaag
    18541 tgcagggcga tattcaggtg gtgcaaaact tcgttgcgct ggcagatctg gctgagttcg
    18601 accctgcgga actgctggcc ccttataccg gtgatatcgc cgctgaaggt atcagcaaag
    18661 ccctgcgcgg gggcgcaaag ttcctgcatc acggcattaa gcgccagcaa cgttatgtgg
    18721 cggaagccat tactgaagag tggcgtatgg cacccggtcc gcttgaagtg gcctggtttg
    18781 cggaagagac ggctgccgtc gagcgtgctg ttgatgccct gaccaaacgg ctggaaaaac
    18841 tggaggctaa atgacgccag gtgaagtacg gcgcctatat ttcatcattc gcactttttt
    18901 aagctacgga cttgatgaac tgatccccaa aatgcgtatc accctgccgc tacggctatg
    18961 gcgatactca ttattctgga tgccaaatcg gcataaagac aaacctttag gtgagcgact
    19021 acgactggcc ctgcaagaac tgggaccagt atggatcaag ttcgggcaga tgttatcaac
    19081 ccgccgcgat ctttttccgc cgcatattgc cgatcagctg gcgttattgc aggacaaagt
    19141 cgctccgttt gatggcaagc tggcgaagca gcagattgaa gctgcaatgg gcggcttgcc
    19201 ggtagaagcg tggtttgacg attttgaaat caagccgctg gcttctgctt ctatcgccca
    19261 ggttcatacc gcgcgattga aatcgaatgg taaagaggtg gtgattaaag ttatccgccc
    19321 ggatattttg ccggtcatta aagcggatct gaaacttatc taccgtctgg ctcgctgggt
    19381 gccgcgtttg ctgccggatg gtcgccgtct gcgcccaacc gaagtggtgc gcgagtacga
    19441 aaagaccttg attgatgaac tgaatttgct gcgggaatct gccaacgcca ttcagcttcg
    19501 acgcaatttt gaagacagcc cgatgctcta catcccggaa gtttaccctg actattgtag
    19561 tgaagggatg atggtgatgg agcgtattta cggcattccg gtgtctgatg ttgcgacgct
    19621 ggagaaaaac ggcaccaaca tgaaattgct ggcggaacgc ggcgtgcagg tgttcttcac
    19681 tcaggtcttt cgcgacagct ttttccatgc tgatatgcac cctggcaaca tcttcgtaag
    19741 ctatgaacac ccggaaaacc cgaaatatat cggcattgat tgcgggattg ttggctcgct
    19801 aaacaaagaa gataaacgct atctggcgga aaactttatc gccttcttta atcgcgacta
    19861 tcgcaaagtg gcagagctac acgtcgattc tggttgggtg ccaccagata ccaacgttga
    19921 agagttcgaa tttgccattc gtacggtctg tgaacctatc tttgagaaac cgctggccga
    19981 aatttcgttt ggacatgtac tgttaaatct gtttaatacg gcgcgtcgct tcaatatgga
    20041 agtgcagccg caactggtgt tactccagaa aaccctgctc tacgtcgaag gggtaggacg
    20101 ccagctttat ccgcaactcg atttatggaa aacggcgaag cctttcctgg agtcgtggat
    20161 taaagatcag gtcggtattc ctgcgctggt gagagcattt aaagaaaaag cgccgttctg
    20221 ggtcgaaaaa atgccagaac tgcctgaact ggtttacgac agtttgcgcc agggcaagta
    20281 tttacagcat agtgttgata agattgcccg cgagcttcag tcaaatcatg tacgtcaggg
    20341 acaatcgcgt tattttctcg gaattggcgc tacgttagta ttaagtggca cattcttgtt
    20401 ggtcagccga cctgaatggg ggctgatgcc cggctggtta atggcaggtg gtctgatcgc
    20461 ctggtttgtc ggttggcgca aaacacgctg attttttcat cgctcaaggc gggccgtgta
    20521 acgtataatg cggctttgtt taatcatcat ctaccacaga ggaacatgta tgggtggtat
    20581 cagtatttgg cagttattga ttattgccgt catcgttgta ctgctttttg gcaccaaaaa
    20641 gctcggctcc atcggttccg atcttggtgc gtcgatcaaa ggctttaaaa aagcaatgag
    20701 cgatgatgaa ccaaagcagg ataaaaccag ccaggatgct gattttactg cgaaaactat
    20761 cgccgataag caggcggata cgaatcagga acaggctaaa acagaagacg cgaagcgcca
    20821 cgataaagag caggtgtaat ctgtgtttga tatcggtttt agcgaactgc tattggtgtt
    20881 catcatcggc ctcgtcgttc tggggccgca acgactgcct gtggcggtaa aaacggtagc
    20941 gggctggatt cgtgcgttgc gttcactggc gacaacggtg cagaacgaac tgacccagga
    21001 gttaaaactc caggagtttc aggacagtct gaaaaaggtt gaaaaggcga gcctcactaa
    21061 cctgacgccc gaactgaaag cgtcgatgga tgaattacgc caggctgcgg agtcgatgaa
    21121 gcgttcctac gttgcaaacg atcctgaaaa ggcgagcgat gaagcgcaca ccatccataa
    21181 cccgatggtg aaagacaatg aaactgtgca tgaaggcgta acgcctgctg ctgcacaaac
    21241 gcaggccagt tcgccggaac agaagccaga aaccacgcca gagccggtgg taaaacctgc
    21301 tgcggacgct gaaccgaaaa ccgctgcacc ttccccttcg tcgagtgata aaccgtaaac
    21361 atgtctgtag aagatactca accgcttatc acgcatctga ttgagctgcg taagcgtctg
    21421 ctgaactgca ttatctcggt gatcgtgata ttcctgtgtc tggtctattt cgccaatgac
    21481 atctatcacc tggtatccgc gccattgatc aagcagttgc cgcaaggttc aacgatgatc
    21541 gccaccgacg tggcctcgcc gttctttacg ccgatcaagc tgacctttat ggtgtcgctg
    21601 attctgtcag cgccggtgat tctctatcag gtgtgggcgt ttatcgcccc agcgctgtat
    21661 aagcatgaac gtcgcctggt ggtgccgctg ctggtttcca gctctctgct gttttatatc
    21721 ggcatggcgt tcgcctactt tgtggtcttt ccgctggcat ttggcttcct tgccaatacc
    21781 gcgccggaag gggtacaggt atccaccgac atcgcgagct atttaagctt cgttatggcg
    21841 ctgtttatgg cgtttggtgt ctcctttgaa gtgccggtgg caattgtgct gctgtgctgg
    21901 atggggatta cctcgccaga agacttacgc aaaaaacgcc cgtatgtgct ggttggtgca
    21961 ttcgttgtcg ggatgttgct gacgccgccg gatgtcttct cgcaaacgct gttggcgatc
    22021 ccgatgtact gcctgtttga aatcggtgtc ttcttctcac gcttttacgt tggtaaaggg
    22081 cgaaaccggg aagaggaaaa cgacgctgaa gcagaaagcg aaaaaactga agaataaatt
    22141 caaccgcccg tcagggcggt tgtcatatgg agtacaggat gtttgatatc ggcgttaatt
    22201 tgaccagttc gcaatttgcg aaagaccgtg atgatgttgt agcgcgcgct tttgacgcgg
    22261 gagttaatgg gctactcatc accggtacca atctgcgtga aagccagcag gcgcaaaagc
    22321 tggcgcgtca gtattcgtcc tgttggtcaa cggcgggcgt acatcctcac gacagcagcc
    22381 agtggcaagc tgtgactgaa gaagcgatta ttgagctggc cgcgcagcca gaagtggtgg
    22441 cgattggtga atgtggtctc gactttaacc gcaacttttc gacgccggaa gagcaggaac
    22501 gcgcttttgt tgcccagcta cgcattgccg cagaattaaa catgccggta tttatgcact
    22561 gtcgcgatgc ccacgagcgg tttatgacat tgctggagcc gtggctggat aaactgcctg
    22621 gtgcggttct tcattgcttt accggcacac gcgaagagat gcaggcgtgc gtggcgtgtg
    22681 gaatttatat cggcattacc ggttgggttt gcgatgaacg acgcgggctg gagctgcggg
    22741 aattgttgcc gttgattccg gcggagaaat tgctgatcga aactgatgcg ccgtatctgc
    22801 tccctcgcga tctcacgcca aagccatcat cccggcgcaa cgcgccagcc catctgcccc
    22861 atattttgca acgtattgcg cactggcgtg gagaagatgc cgcatggctg gctgccacca
    22921 cggatgccaa tgtcaaaaca ctgtttggga ttgcgtttta gagtttgcgg aactcggtgt
    22981 tctttacgct gtgcttaatt tctttattga taagattaag caatagcatg gagcgagcct
    23041 ctccgtctgg ctcggtgaaa atggcctgaa aaccttcgaa tgcgccttca gtaataatca
    23101 ctttatcacc aggatacggc gtcgatggat cgacaatatc tttcggttta tacactgata
    23161 actgatgaat caccgccgat gggactatcg ctggcgacgc gccaaagcgc acgaagtggc
    23221 tgacaccgcg ggtcgcgttg atagtcgtgg tatgaatcac ttccggatcg aactccacaa
    23281 acaagtagtt ggggaacaat ggctcactga ctgcagtacg tttgccacgc acgatttttt
    23341 ccagggtgat catcggtgcc aggcaattca cagcctgtct ttcgaggtgt tcctgggcac
    23401 gttgaagttg cccgcgcttg cagtacagta aataccagga ttgcataatg actcttatcc
    23461 gtttaatcgg ggcgcaagga tagcaaaagc tttacgctaa gttaattata ttccccggtt
    23521 tgcgttatac cgtcagagtt cacgctaatt taacaaattt acagcatcgc aaagatgaac
    23581 gccgtataat gggcgcagat taagaggcta caatggacgc catgaaatat aacgatttac
    23641 gcgacttctt gacgctgctt gaacagcagg gtgagctaaa acgtatcacg ctcccggtgg
    23701 atccgcacct ggaaatcact gaaattgctg accgcacttt gcgtgccggt gggcctgcgc
    23761 tgttgttcga aaaccctaaa ggctactcaa tgccggtgct gtgcaacctg ttcggtacgc
    23821 caaagcgcgt ggcgatgggc atggggcagg aagatgtttc ggcgctgcgt gaagttggta
    23881 aattattggc gtttctgaaa gagccggagc cgccaaaagg tttccgcgac ctgtttgata
    23941 aactgccgca gtttaagcaa gtattgaaca tgccgacaaa gcgactgcgt ggtgcaccct
    24001 gccaacaaaa aatcgtctct ggcgatgacg tcgatctcaa tcgcattccc attatgacct
    24061 gctggccgga agatgccgcg ccgctgatta cctgggggct caccgtaacg cgcggcccgc
    24121 ataaagagcg gcagaatctg ggcatttatc gccagcagct aattggtaaa aacaaactga
    24181 ttatgcgctg gctgtcgcat cgcggcggcg cgttggatta tcaggagtgg tgtgcggcgc
    24241 atccgggcga acgtttcccg gtttctgtgg cgctgggtgc cgatcctgcc acgattctcg
    24301 gtgcagtcac ccccgttccg gatacgcttt cagagtatgc gtttgccgga ttgctgcgcg
    24361 gtaccaagac cgaagtggtg aagtgtatct ccaatgacct tgaagtgccc gccagtgcgg
    24421 agattgtgct ggaagggtat atcgaacaag gcgaaactgc gccggaaggg ccgtatggcg
    24481 accataccgg ttactataac gaagtcgata gttttccggt atttaccgtg acgcatatta
    24541 cccagcgtga agatgcgatt tatcattcca cctataccgg gcgtccgcca gatgaacctg
    24601 cggtactggg tgtagcactg aacgaagtgt tcgtgccgat tctgcaaaaa cagttcccgg
    24661 aaattgtcga tttttatctg ccgccggaag gctgttctta tcgtctggcg gtagtgacga
    24721 tcaaaaaaca gtacgccgga cacgcgaagc gcgtcatgat gggcgtctgg tcgttcttac
    24781 gccagtttat gtacactaaa tttgtgatcg tttgcgatga tgacgtcaac gcccgcgact
    24841 ggaacgatgt gatttgggcg attaccaccc gtatggaccc ggcgcgggat actgttctgg
    24901 tagaaaatac gcctattgat tatctggatt ttgcctcgcc tgtctccggg ctgggttcaa
    24961 aaatggggct ggatgccacg aataaatggc cgggtgaaac ccagcgtgaa tggggacgtc
    25021 ccatcaaaaa agatccagat gttgtcgcgc atattgacgc catttgggat gaactggcta
    25081 tttttaacaa cggtaaaagc gcctgatgcg cgtttgtttt gccctattta tcgatccgac
    25141 agagaaagcg catgacaacc ttaagctgta aagtgacctc ggtagaagct atcacggata
    25201 ccgtatatcg tgtccgcatc gtgccagacg cggccttttc ttttcgtgct ggtcagtatt
    25261 tgatggtagt gatggatgag cgcgacaaac gtccgttctc aatggcttcg acgccagatg
    25321 aaaaagggtt tatcgagctg catattggcg cttctgaaat caacctttac gcgaaagcag
    25381 tcatggaccg catcctcaaa gatcatcaaa tcgtggtcga tattccccac ggagaagcgt
    25441 ggctgcgcga tgatgaagag cgtccgatga ttttgattgc gggcggcacc gggttctctt
    25501 atgcccgctc gattttgctg acagcgttgg cgcgtaaccc aaaccgtgat atcaccattt
    25561 actggggcgg gcgtgaagag cagcatctgt atgatctctg cgagcttgag gcgctttcgt
    25621 tgaagcatcc tggtctacaa gtggtgccgg tggttgaaca accggaagcg ggctggcgtg
    25681 ggcgtactgg caccgtgtta acggcggtat tgcaggatca tggtacgctg gcagagcatg
    25741 atatctatat tgccggacgt tttgagatgg cgaaaattgc ccgtgacctg ttttgcagcg
    25801 agcgtaatgc gcgggaagat cgcctgtttg gcgatgcgtt tgcatttatc tgagatataa
    25861 aaaacccgcc cctgacaggc gggaagaacg gcaactaaaa ctttatacag tggcattact
    25921 ctacgttgca tccggcaaac agtgcccgat gcaacgctgc cgcgtcttat cgggcctaca
    25981 aaagttctga accgtgggcc ggataaggcg ttcacgccgc atccggcaag tggttaaacc
    26041 cgctcaaaca ccgttgcaat gccctgaccc agaccgatac acatcgtcgc cagaccaaac
    26101 tggacgtctt tgtgttccat cagattcagc agcgtggtgc tgatacgcgc accggaacaa
    26161 cccagcggat gacccagcgc gatcgcgcca ccgttgaggt tgatcttctc gtcaatctgc
    26221 tccattagcc cgagatcttt aatacacggc aggatctgcg cggcaaaggc ttcgttcatc
    26281 tcgaatacgc cgatatcgct ggcagaaagc cccgcttttt tcagcgccag cttcgaggcc
    26341 ggaaccggac cataacccat aatagatggg tcacaaccca caaccgccat cgaacgcacg
    26401 cgggcgcgcg gcttaagacc taattcacgg gcgcggcttt cactcatcac cagcatggca
    26461 gctgcgccat cggaaagcgc agaagatgta cccgccgtta ccgtaccgct gacgggatca
    26521 aacgccggac gcagtgtggc gagggcttcc acggtggttt ccgggcgaat cacttcgtcg
    26581 taattaaact gcttcaatac gccgtcggca tcgtgaccac cggtcgggat gatttcattt
    26641 ttaaatgcgc ctgactgcgt ggcggcccac gcgcgggcgt gtgaccgcgc ggcaaaggca
    26701 tcctgcattt cacggctgat accgtgcata cgcgccagca tttctgccgt taagcccatc
    26761 atgcccgccg ctttggcgac attacggctc aggccggggt gaaaatcgac gccgtgactc
    26821 attggcacat ggcccatatg ctccacgccg ccaaccagac atgcctgcgc atcaccggtc
    26881 atgatcattc gtgctgcgtc atgcagtgcc tgcatggatg aaccacacaa gcgattaacg
    26941 gtaaccgccg ggacagagtg cggcacttca gccaacagtg ccgcgttacg ggcgatatta
    27001 aaaccctgct ccagtgtttg ctgcacacaa ccccagtaaa tatcgtcaag ggccgccgct
    27061 tccagtgccg ggttacgcgc cagcaggcta cgcattaaat gagcggagag atcttccgca
    27121 cgcacgttac gaaaagcacc gcccttcgaa cggcccatcg gggtgcgaat tgcatcgaca
    27181 atgacaacct gttccattgt gactccttaa gccgttttca ggtcgccaac cggacgggct
    27241 ggctcaaccg ggggatagta cggttcgtta tgacgtgctt tattacgcag accttccggt
    27301 acttcataca gcgggccgag gtgctgatat tgctgtgcca tatcgaggta ttttgcgctg
    27361 ccgagggtgt ccagccagcg gaacgcgccg ccgtggaacg gagggaagcc caggccgtag
    27421 accagcgcca tatccgcttc tgccggagtg gcgataatgc cctcttccag acagcggacc
    27481 acttcgttga ccatcgggat catcatgcgg gcgataatct cttcttcgct gaaatcgcgc
    27541 ttcggctggc tcacttttgc cagcaggtcg tcaacgacga catcttcttc tttcttcggc
    27601 ttacctttgc tgtcttcttt ataacgccag aaaccgaggc cgttcttctg accaaagcgg
    27661 ttggcatcaa acagcgcgtc gatggcatcg cgataatctt tctgcatccg ctgcgggaag
    27721 cctgctgcca tgacagcctg ggcgtgatgc gcggtgtcaa tgcccacaac atccagcaga
    27781 tatgccggac ccatcggcca gccaaactgt ttttccatca ctttgtcgat cttgcggaaa
    27841 tccgcgccgt cgcgcagcaa ctggctgaaa ccggcgaaat aggggaacag tacacggttg
    27901 acgaagaagc cggggcagtc gttaaccaca atcggcgttt tgcccatctt gctcgcccag
    27961 gcgacgactt tcgcgatggt ttcgtcggag cttttctcgc cgcgaataat ttctaccaac
    28021 ggcattcggt ggaccgggtt aaagaagtgc atcccgcaga agttttccgg acgttccagc
    28081 gcgttggcca gttcgctgat aggaatggtt gaagtgttcg acgccagtac ggtatccggg
    28141 cgtactttct gctcggtttc tgccagcacg gcttttttca ctttcgggtt ttcaacaacc
    28201 gcttctacca caacatccac gcggtcaaac ccggcgtagt cgagcgttgg gtgaattgtg
    28261 gagatcacgc cagccagttt cagaccatcg atcttgccgc gctcaagctg cttgttcagc
    28321 agtttcgcgg cttcggtcat gccgagggtt aacgacttgt cgttgatatc tttcatgaca
    28381 accggcacgc ctttccacgc agactggtaa gcgatgccgc cgcccataat gcctgcgccc
    28441 agcacagcgg cctgtttcgg ggtttcaaca tctttggtga gtttcttcgc tttacctttt
    28501 acatattgat cgttaaggaa aatgccgacc agtgcgcggg cttcgttggt atgcgccagc
    28561 gggacaaaac ttttgttttc caggtttaag gcttcttcac gaccaaaacg ggccgcagct
    28621 tcaatggttt ttactgcggt gatgggggcc ggataatgtt tccccgccgt ttgtgcgacc
    28681 atccctttag cgatggtgaa gctcatggtg gcttcaatct tgctcagttt tagcggttcc
    28741 agcttcggct gacgtttcgc tttccagtcg aggtcgccgt taatggcctg gcgtaaaatc
    28801 gccatcgccc cttcaatcag tttttctgct ttgactacac catccaccag accgattttc
    28861 agcgcctgat ccgcgccgac atctttaccg gcggcaatga tttccagcgc actgtcagcg
    28921 cccagcatac gtggcatacg tacagaaccg ccaaagcccg gcatgatgcc cagtttggtt
    28981 tccggcagac cgatgcgcag atccggcgtc gccagacgat aatcggtcgc cagcacgcat
    29041 tcgcagccac cgcccagcgc atagccgttg acggcagcaa tggtcggcac cgggagatct
    29101 tccaggcgat taaacacgct attggcaaag tgcagccact gacttaactg ttcttcagga
    29161 acgaggaaca gggacaaaaa ttcggtgata tcagcaccga cgataaaggc tgctttgttc
    29221 gaacgcagca gcagcccttt tagatctgat tgctgttcca gcacgccgat ggcctcgccg
    29281 aggctggcga cggtcgcagt gtcgagttta ttaactgaac ctggggcatc aaataccagt
    29341 tcggcaatgc catcttccag ccagtcaagg tacagggtgt cgcctttgta aagcatgtca
    29401 gtctcctgaa tccgcaaggt gatctggtcg taccagatgc atcgaagtgt gtattttgtg
    29461 ttaaaaatat gcaaataaaa gattaaagaa atgccgatct gatcacgctc ggcagaaatc
    29521 acgctctgga tgaacgatgt gctaagatgc ggagacttaa ggtcaaaaaa acagaagggt
    29581 aaaaaatgga atcactggcc tcactctata aaaatcatat cgctacctta caagaacgga
    29641 ctcgcgatgc gctggcgcgc ttcaagctgg atgcgttact tattcactcc ggcgaactgt
    29701 tcaacgtttt tctcgacgat catccctatc cgtttaaagt gaacccgcaa ttcaaagcgt
    29761 gggtgccggt aactcaggtg ccaaactgct ggctgctggt ggatggcgtg aacaagccga
    29821 aactgtggtt ttatctgccg gttgattact ggcacaacgt cgaaccgcta ccgacctcct
    29881 tctggactga agatgtagaa gtgatcgcgc tgccgaaagc cgatggcatt ggtagcctgc
    29941 tgcctgctgc gcgcggcaat atcggttata tcggtccggt gccggaacgt gcgctgcaac
    30001 tgggtattga ggccagcaac atcaacccga aaggggtgat cgactacctg cattactacc
    30061 gctccttcaa aaccgagtac gaactggcct gtatgcgtga agcgcagaaa atggcggtca
    30121 atggtcatcg tgcggcagaa gaagcgttcc gttctggcat gagcgagttc gatatcaaca
    30181 ttgcctatct gaccgcgacc ggtcatcgtg ataccgacgt accttatagc aacattgtgg
    30241 cgcttaacga acacgctgcg gtgttgcatt acaccaaact ggaccaccag gcaccggaag
    30301 agatgcgcag cttcctgctg gatgccgggg ccgaatataa cggctatgcg gctgatctga
    30361 cccgtacctg gtcggcaaaa agcgacaacg actacgcaca gctggtgaaa gacgtaaatg
    30421 atgagcaact ggcgctgatc gccaccatga aagcaggcgt cagctatgtg gattaccata
    30481 tccagttcca tcagcgcatc gccaaactgc tgcgtaaaca tcaaatcatc accgatatga
    30541 gtgaagaagc gatggtcgaa aacgatctca ccggaccgtt tatgccgcac ggtatcggcc
    30601 atccgctggg cctgcaggtg catgacgtag ccggttttat gcaggatgat agcggtacgc
    30661 acctcgcggc accggcaaaa tatccgtacc tgcgctgcac ccgtattctc cagccgggca
    30721 tggtgttaac catcgaaccg ggtatctact tcatcgaatc gctgctggca ccgtggcgtg
    30781 aagggcagtt cagcaaacac ttcaactggc agaaaattga agcactgaaa ccgttcggcg
    30841 gcattcgtat cgaagacaac gtggtgattc acgaaaacaa cgtggaaaac atgacccggg
    30901 atctgaaact ggcgtgatgg aaagctggtt aattcctgcg gcaccggtca cggtcgttga
    30961 agagatcaaa aagagccgct tcattacgct gttggcgcat accgatggcg ttgaggcggc
    31021 aaaagcgttt gttgaatcgg tgcgggcaga acaccccgat gcccgccacc attgcgtggc
    31081 gtgggtggcg ggtgcgccgg atgattctca acagctgggt ttctctgacg acggggagcc
    31141 ggcgggaacg gcaggtaaac cgatgctcgc ccaactaatg ggcagcggcg tcggggaaat
    31201 taccgctgtg gtggtgcgct actacggcgg catattgctt ggcaccggtg ggttagtgaa
    31261 agcgtatggc ggcggcgtga atcaggcgct gcgccagcta acgacccaac gcaagacacc
    31321 gttaaccgaa tatactttgc aatgtgaata tagtcagtta accggcattg aagcgttgct
    31381 ggggcagtgt gacggcaaaa ttatcaacag tgattatcag gcattcgttc tgctgcgggt
    31441 ggcgcttccg gcggcgaaag tggctgaatt ttccgcaaag ctggcggatt ttagccgtgg
    31501 ttcattgcaa ttgttagcga ttgaagaata atccccactt cgttttgcag aactaaggaa
    31561 gcggcagaga tgcattttcg cgccattacc cgaatcgttg gactactggt tatcttattt
    31621 tcagggacca tgattatccc tgggctggta gcactcatct accgggatgg agcgggccgc
    31681 gcttttaccc agaccttttt tgtcgccctc gccattggct ctatgctttg gtggccgaac
    31741 cgcaaagaga agggtgaact gaaatcccgt gaggggtttc tgatagtggt gctgttctgg
    31801 accgtgctgg gtagcgtcgg tgcgctccct tttatcttct cggaaagccc gaacctcacg
    31861 attaccgatg cgttttttga atctttctct ggcctgacca ccacgggggc cactacgctg
    31921 gtggggctgg attcgctccc tcacgccatc ctcttttatc gccagatgct gcaatggttt
    31981 ggcgggatgg ggatcatcgt gttggcggtt gcgatactgc ctatcctcgg cgtgggtggg
    32041 atgcagctct atcgcgcaga aatgcccggc ccgctgaaag ataacaaaat gcgcccgcga
    32101 attgcggaaa cggcgaaaac cctgtggttg atttatgtct tgctgaccgt cgcctgtgcg
    32161 ctggcgttgt ggtttgctgg aatggatgcc tttgatgcca tcggccatag ctttgcgact
    32221 atcgctattg gcggcttctc gacacatgat gccagtatcg gttatttcga cagcccgact
    32281 attaacacta tcattgctat cttcctgctg atctctggct gtaactacgg tctgcacttt
    32341 tcactgttaa gtgggcgtag tctgaaggtt tattggcgcg atccggaatt tcgcatgttt
    32401 atcggcgtac agttttcact ggtggttatt tgtacactcg tactgtggtt tcataatgtc
    32461 tacagttcgg cgctgatgac aattaaccag gcgtttttcc aggtggtgtc gatggcgaca
    32521 accgccgggt ttacaactga cagcattgcc cgctggccgc tctttttgcc ggtactgctt
    32581 ttatgttcag cttttatcgg cggttgcgcc gggtcaacgg gcggtggcct gaaagtgatc
    32641 cgcatcctgt tgctgtttaa gcaggggaac cgtgagctga aacgactggt gcatccgaac
    32701 gccgtctata gcattaagct ggggaatcgc gcactgccgg aacgtatcct cgaagccgtt
    32761 tggggatttt tctccgccta tgcattggtg tttattgtca gtatgctggc gattatcgcc
    32821 acgggcgtgg atgacttttc tgcctttgcc tcggttgttg caacattgaa taacctgggg
    32881 ccagggcttg gcgtggttgc tgataacttt accagtatga acccggtggc taaatggatc
    32941 ctgattgcca acatgctgtt tggtcgtctc gaggtcttta cattgctggt gctctttacc
    33001 ccgactttct ggcgtgaatg atggagtaat acgtgaaaac attaattctt ttctcaacaa
    33061 gggacggaca aacgcgcgag attgcctcct acctggcttc ggaactgaaa gaactgggga
    33121 tccaggcgga tgtcgccaat gtgcaccgca ttgaagaacc acaatgggaa aactatgacc
    33181 gtgtggttat tggtgcttct attcgctatg gtcactacca ctctgcgttc caggaatttg
    33241 tcaaaaagca tgcgacgcga ctgaattcga tgccgagcgc cttttattcc gtaaatctgg
    33301 tggcgcgtaa accggagaag cgtactccac agaccaacag ctacgcgcgg aagtttctga
    33361 tgaactcgca atggcgtccc gatcgctgtg ccgtcattgc cggggcgctg cgttacccac
    33421 gttatcgctg gtacgaccgt tttatgatca agctgattat gaagatgtca ggcggtgaaa
    33481 cggatacgcg caaagaagtt gtctataccg attgggagca ggtggcgaat ttcgcccgag
    33541 aaatcgccca tttaaccgac aaaccgacgc tgaaataagc ataaagaata aaaaatgcgc
    33601 ggtcagaaaa ttattttaaa tttcctcttg tcaggccgga ataactccct ataatgcgcc
    33661 accactgaca cggaacaacg gcaaacacgc cgccgggtca gcggggttct cctgagaatc
    33721 tcaacagaga aaagcaaaaa aatgcttgac tctgtagcgg gaaagcgtat tatgcacacc
    33781 ccgcgccgct gagaaaagcg aagcggcact gctctttaac aatttatcag acaatctgtg
    33841 tgggcactcg aagatacgga ttcttgacgt cgcaagacga aaaatgaata ccaagtctca
    33901 ggagtgaaca cgtaattcat tacgaagttt aattctttga gcatcaaact tttaaattga
    33961 agagtttgat catggctcag attgaacgct ggcggcaggc ctaacacatg caagtcgaac
    34021 ggtaacagga agcagcttgc tgctttgctg acgagtggcg gacgggtgag taatgtctgg
    34081 gaaactgcc
//
LOCUS       DAHHTG010000043        32851 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.043, whole genome shotgun sequence.
ACCESSION   DAHHTG010000043 DAHHTG010000000
VERSION     DAHHTG010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 32851)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32851)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32851
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.043"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            complement(44..2029)
                     /gene="yjcS"
                     /locus_tag="NBI74_002004"
     CDS             complement(44..2029)
                     /gene="yjcS"
                     /locus_tag="NBI74_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl sulfatase YjcS"
                     /protein_id="HCD1932347.1"
                     /translation="MNNSRLFRLSRIVIAFTAASGMMINTAYATDEAKAATQYTQQVN
                     QNYAKSLPFSDRQDFDDALRGFIAPLLDEGILRDANGKIYYRANDYKFDINAAAPETV
                     NPSLWRQSQINGISGLFKVTDKMYQVRGQDISNITFVEGEKGIIVIDPLVTPPAAKAA
                     LDLYFQHRPQKPIVAVIYTHSHADHYGGVKGIISEADVKSGKVQVIAPAGFMDEAISE
                     NVLAGNIMSRRALYSYGLLLPHNAQGNVGNGLGVTLATGDPSIIAPTKTIVRTGEKMI
                     IDGLEFDFLMTPGSEAPAEMHFYIPALKALCTAENATHTLHNFYTLRGAKTRDTSKWT
                     EYLNETLDMWGNDAEVLFMPHTWPVWGNKHINDYIGKYRDTIKYIHDQTLHLANQGYT
                     MNEIGDMIKLPPALSNNWASRGYYGSVSHNARAVYNFYLGYYDGNPANLHPYGQVEMG
                     KRYVQALGGSARVINLAQEANKQGDYRWSAELLKQVIAANPGDQVAKNLQANNFEQLG
                     YQAESATWRGFYLTGAKELREGVHKFSHGTTGSPDTLRGMSVEMLFDFMAVRLDSAKA
                     AGKNISLNFNMSNGDNLNLTLNDSVLNYRKTLQPQADASFYISREDLHAVLTGQAKMA
                     DLVKAKKAKIIGNSAKLEEIIACLDNFDLWVNIVTPN"
     gene            complement(2302..3231)
                     /gene="alsK"
                     /locus_tag="NBI74_002005"
     CDS             complement(2302..3231)
                     /gene="alsK"
                     /locus_tag="NBI74_002005"
                     /EC_number="2.7.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="allose kinase"
                     /protein_id="HCD1932348.1"
                     /translation="MQKQHNVVAGVDMGATHIRFCLRTAEGETLHCEKKRTAEVIAPD
                     LVSGIGEMIDEQLRRFNARCHGLVMGFPALVSKDKRTIISTPNLPLTAADLYDLADKL
                     ENTLNCPVEFSRDVNLQLSWDVVENRLTQQLVLAAYLGTGMGFAVWMNGAPWTGAHGV
                     AGELGHIPLGDMTQHCACGNPGCLETNCSGMALRRWYEQQSRNYPLSDLFVHAENAPF
                     VQSLLENAARAIATSINLFDPDAVILGGGVMDMPAFPRETLIAMTQKYLRRPLPYQVV
                     RFIAASSSDFNGAQGAAILAHQRFLPQSCAKVP"
     gene            complement(3215..3910)
                     /gene="alsE"
                     /locus_tag="NBI74_002006"
     CDS             complement(3215..3910)
                     /gene="alsE"
                     /locus_tag="NBI74_002006"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-allulose-6-phosphate 3-epimerase"
                     /protein_id="HCD1932349.1"
                     /translation="MKISPSLMCMDLLKFKEQIEFIDSHADYFHIDIMDGHFVPNLTL
                     SPFFVSQVKKLASKPLDCHLMVTRPQDYIAQLARAGADFITLHPETINGQAFRLIDEI
                     RRHGMKVGLILNPETPVEAMKYYIHKADKITVMTVDPGFAGQPFIPEMLDKVAELKAW
                     REREGLEYEIEVDGSCNQATYEKLMAAGADVFIVGTSGLFNHAENIDEAWRIMTAQIL
                     AAKSEVQPHAKTA"
     gene            complement(3921..4901)
                     /gene="alsC"
                     /locus_tag="NBI74_002007"
     CDS             complement(3921..4901)
                     /gene="alsC"
                     /locus_tag="NBI74_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-allose ABC transporter permease"
                     /protein_id="HCD1932350.1"
                     /translation="MGFTTRVKSEASEKKPFNFALFWDKYGTFFILAIIVAIFGSLSP
                     EYFLTTNNITQIFVQSSVTVLIGMGEFFAILVAGIDLSVGAILALSGMVTAKLMLAGI
                     DPFLAALIGGVLVGGALGAINGCLVNWTGLHPFIITLGTNAIFRGITLVISDANSVYG
                     FSFDFVNFFAASVIGIPVPVIFSLIVALILWFLTTRMRLGRNIYALGGNKNSAFYSGI
                     DVKFHILVVFIISGVCAGLAGVVSTARLGAAEPLAGMGFETYAIASAIIGGTSFFGGK
                     GRIFSVVIGGLIIGTINNGLNILQVQTYYQLVVMGGLIIAAVALDRLISK"
     gene            complement(4880..6412)
                     /gene="alsA"
                     /locus_tag="NBI74_002008"
     CDS             complement(4880..6412)
                     /gene="alsA"
                     /locus_tag="NBI74_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-allose ABC transporter ATP-binding protein
                     AlsA"
                     /protein_id="HCD1932351.1"
                     /translation="MATPYISMAGIGKSFGPVHALKSVNLTVYPSEIHALLGENGAGK
                     STLMKVLSGIHEPTKGTITINNINYNKLDHKLAAQLGIGIIYQELSVIDELTVLENLY
                     IGRHLTKKICGVNIIDWREMRVRAAMMLLRVGLKVDLDEKVANLSISHKQMLEIAKTL
                     MLDAKVIIMDEPTSSLTNKEVDYLFLIMNQLRKEGTAIVYISHKLAEIRRICDRYTVM
                     KDGSSVCSGMVSDVSNDDIVRLMVGRELQNRFNAMKENVSNLAHDTVFEVRNVTSRDR
                     KKVRDISFSVCRGEILGFAGLVGSGRTELMNCLFGVDKRAGGEIRLNGKDISPRSPLD
                     AVKKGMAYITESRRDNGFFPNFSIAQNMAISRSLKDGGYKGAMGLFHEVDEQRTAEAQ
                     RELLALKCHSVNQNITELSGGNQQKALISKWLCCRPEVIIFDEPTRGIDVGAKAEIYK
                     VMRQLADDGKVILMVSSELPEIITVCDRIAVFCEGRLTQILTNRDDMSEEEIMAWALP
                     QE"
     gene            complement(6539..7474)
                     /gene="alsB"
                     /locus_tag="NBI74_002009"
     CDS             complement(6539..7474)
                     /gene="alsB"
                     /locus_tag="NBI74_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-allose transporter substrate-binding protein"
                     /protein_id="HCD1932352.1"
                     /translation="MNKYLKYFSGTLVGLMLSTSAFAAAEYAVVLKTLSNPFWVDMKK
                     GIEDEAKTLGVSVDIFASPSEGDFQSQLQLFEDLSNKNYKGIAFAPLSSVNLVMPVAR
                     AWKKGIYLVNLDEKIDMDNLKKAGGNVEGFVTTDNVAVGAKGASFIIDKLGAEGGEVA
                     IIEGKAGNASGEARRNGATEAFKKASQIKLVASQPADWDRIKALDVATNVLQRNPNIK
                     AIYCANDTMAMGVAQAVANAGKTGKVLVVGTDGIPEARKMVEAGQMTATVAQNPADIG
                     ATGLKLMVDAEKSGKVIPLDKAPEFKLVDSILVTQ"
     gene            complement(7533..8423)
                     /gene="alsR"
                     /locus_tag="NBI74_002010"
                     /gene_synonym="rpiR"
     CDS             complement(7533..8423)
                     /gene="alsR"
                     /locus_tag="NBI74_002010"
                     /gene_synonym="rpiR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="als operon DNA-binding transcriptional repressor
                     AlsR"
                     /protein_id="HCD1932353.1"
                     /translation="MSQSEFDSALPNGIGLAPYLRMKQEGMTENESRIVEWLLKPGNL
                     SCAPAIKDVAEALAVSEAMIVKVSKLLGFSGFRNLRSALEDYFSQSEQVLPSELAFDE
                     APQDVVNKVFNITLRTIMEGQSIVNVDEIHRAARFFYQARQRDLYGAGGSNAICADVQ
                     HKFLRIGVRCQAYPDAHIMMMSASLLQEGDVVLVVTHSGRTSDVKAAVELAKKNGAKI
                     ICITHSYHSPIAKLADYIICSPAPETPLLGRNASARILQLTLLDAFFVSVAQLNIEQA
                     NINMQKTGAIVDFFSPGALK"
     gene            8782..9231
                     /gene="rpiB"
                     /locus_tag="NBI74_002011"
     CDS             8782..9231
                     /gene="rpiB"
                     /locus_tag="NBI74_002011"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional allose-6-phosphate
                     isomerase/ribose-5-phosphate isomerase RpiB"
                     /protein_id="HCD1932354.1"
                     /translation="MKKIAFGCDHVGFILKHEIVAHLVERGVEVIDKGTWSSERTDYP
                     HYASQVALAVAGGEADGGILICGTGVGISIAANKFAGIRAVVCSEPYSAQLSRQHNDT
                     NVLAFGSRVVGLELAKMIVDAWLGAQYEGGRHQQRVEAITAIEQRRN"
     gene            9300..9638
                     /locus_tag="NBI74_002012"
     CDS             9300..9638
                     /locus_tag="NBI74_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1932355.1"
                     /translation="MKRFPLFLLFTLFTLFTLFTVPAQADIIDDTIGNIQQAINDAYN
                     PDRGRDYEDSRDDGWQREVSDDRRRQYDDRRRQFEDRRRQLDERQRQLDQERQQLEDE
                     ERRMEDEYGR"
     gene            complement(9666..10424)
                     /gene="phnP"
                     /locus_tag="NBI74_002013"
     CDS             complement(9666..10424)
                     /gene="phnP"
                     /locus_tag="NBI74_002013"
                     /EC_number="3.1.4.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016234166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate metabolism protein PhnP"
                     /protein_id="HCD1932356.1"
                     /translation="MSLILTLTGTGGAQGVPAWGCECAACARARRSPQYRRQPCSGVV
                     KFNDAITLIDAGLHDLADRWSPGSFQQFLLTHYHMDHVQGLFPLRWGVGDTIPVYGPP
                     DEQGCDDLFKHPGLLDFSHTVEPFVVFDLQGLQVTPLPLNHSKLTFGYLLETAHSRVA
                     WLSDTAGLPEKTLKFLRNNQPQVMVIDCSHPPREDAPRNHCDLNTVLALNQVIRSPRV
                     ILTHISHQFDAWLMENALPSGFEAGFDGMEIEVE"
     gene            complement(10426..10860)
                     /gene="phnO"
                     /locus_tag="NBI74_002014"
     CDS             complement(10426..10860)
                     /gene="phnO"
                     /locus_tag="NBI74_002014"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoalkylphosphonate N-acetyltransferase"
                     /protein_id="HCD1932357.1"
                     /translation="MPACELRPATQYDTDAVYALICELKQAEFDHHAFRVGFNANLRD
                     PNMRYHLALLDGEVVGMIGLHLQFHLHHVNWIGEIQELVVMPQARGLNVGSKLLAWAE
                     EEARQAGAEMTELSTNVKRHDAHRFYLREGYEQSHFRFTKAL"
     gene            complement(10847..11404)
                     /gene="phnN"
                     /locus_tag="NBI74_002015"
     CDS             complement(10847..11404)
                     /gene="phnN"
                     /locus_tag="NBI74_002015"
                     /EC_number="2.7.4.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 1,5-bisphosphokinase"
                     /protein_id="HCD1932358.1"
                     /translation="MTGKLIWLMGASGSGKDSLLTELRQREQTQLLVAHRYITRAASA
                     GSENHIALSEQEFFTRAGQNLLALSWHANGLYYGVGVEIDLWLHAGFDVMVNGSRAHL
                     PQARARYQSALLPVCLQVSPEILRQRLENRGRENASEINARLARAARYTPQDCLTLNN
                     DGSLRQSVDKLLTLIHQKEKHHACL"
     gene            complement(11404..12540)
                     /gene="phnM"
                     /locus_tag="NBI74_002016"
     CDS             complement(11404..12540)
                     /gene="phnM"
                     /locus_tag="NBI74_002016"
                     /EC_number="3.6.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate diphosphatase"
                     /protein_id="HCD1932359.1"
                     /translation="MIINNVKLVLENEVVHGSLEMQDGEIRAFAESQSHLSEAMDGEG
                     GWLLPGLIELHTDNLDKFFTPRPKVDWPAHSAMSSHDALMVASGITTVLDAVAIGDVR
                     DGGDRLENLEKMINAIEETQKRGVNRAEHRLHLRCELPHHTTLPLFEKLVQREPVTLV
                     SLMDHSPGQRQFANREKYREYYQGKYSLTDAQMQQYEEEQLALAARWSQPNRESIAAM
                     CRARHIALASHDDATHAHVAESHQLGSVIAEFPTTFEAAEASRKHGMNVLMGAPNIVR
                     GGSHSGNVAASELAQLGLLDILSSDYYPASLLDAAFRVADDESNRFTLPQAVKLVTKN
                     PAQALNLQDRGVIGEGKRADLVLAHRKGNHIHIDHVWRQGKRVF"
     gene            complement(12537..13217)
                     /gene="phnL"
                     /locus_tag="NBI74_002017"
     CDS             complement(12537..13217)
                     /gene="phnL"
                     /locus_tag="NBI74_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnL"
                     /protein_id="HCD1932360.1"
                     /translation="MINVQNVSKTFILHQQNGVRLPVLQGASLTVNAGECVVLHGHSG
                     SGKSTLLRSLYANYLPDEGQIQIKHGDEWVDLVTAPARKVVEIRKTTVGWVSQFLRVI
                     PRISALEVVMQPLLDTGVPREACAAKAARLLTRLNVPERLWHLAPSTFSGGEQQRVNI
                     ARGFIVDYPILLLDEPTASLDAKNSAAVVELIREAKARGAAIVGIFHDETVREQVADR
                     LHPMGVSS"
     gene            complement(13328..14086)
                     /gene="phnK"
                     /locus_tag="NBI74_002018"
     CDS             complement(13328..14086)
                     /gene="phnK"
                     /locus_tag="NBI74_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnK"
                     /protein_id="HCD1932361.1"
                     /translation="MNQPLLSVNNLTHLYAPGKGFSDVSFDLWPGEVLGIVGESGSGK
                     TTLLKSISARLTPQQGEIRYENRSLYAMSEADRRRLLRTEWGVVHQHPLDGLRRQVSA
                     GGNIGERLMATGARHYGDIRATAQKWLEEVEIPANRIDDLPTTFSGGMQQRLQIARNL
                     VTHPKLVFMDEPTGGLDVSVQARLLDLLRGLVMELNLAVVIVTHDLGVARLLADRLLV
                     MKQGKVVESGLTDRVLDDPHHPYTQLLVSSVLQN"
     gene            complement(14083..14928)
                     /gene="phnJ"
                     /locus_tag="NBI74_002019"
     CDS             complement(14083..14928)
                     /gene="phnJ"
                     /locus_tag="NBI74_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate 5-phosphate
                     C-P-lyase PhnJ"
                     /protein_id="HCD1932362.1"
                     /translation="MANLSGYNFAYLDEQTKRMIRRAILKAVAIPGYQVPFGGREMPM
                     PYGWGTGGIQLTASVIGESDVLKVIDQGADDTTNAVSIRNFFKRVTGVNTTERTDDAT
                     LIQTRHRIPETPLTEDQIVIFQVPIPEPLRFIEPRETETRTMHALEEYGVMQVKLYED
                     IARFGHIATTYAYPVKVNGRYVMDPSPIPKFDNPKMDMMPALQLFGAGREKRIYAVPP
                     FTRVESLDFDDHPFTVQQWDEPCAICGSTHSYLDEVVLDDAGNRMFVCSDTDYCRQQS
                     EAKSQ"
     gene            complement(14921..15985)
                     /gene="phnI"
                     /locus_tag="NBI74_002020"
     CDS             complement(14921..15985)
                     /gene="phnI"
                     /locus_tag="NBI74_002020"
                     /EC_number="2.7.8.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate synthase subunit PhnI"
                     /protein_id="HCD1932363.1"
                     /translation="MYVAVKGGEKAIDAAHALQESRRRGDTDLPELSVAQIEQQLNLA
                     VDRVMTEGGIADRELAALALKQASGDNVEAIFLLRAYRTTLAKLAVSEPLDTTGMRLE
                     RRISAIYKDIPGGQLLGPTYDYTHRLLDFTLLANGETPTLTTADSEQQPSPHVFSLLA
                     RQGLAKFEEDSGAQPDDITRTPPVYPCSRSSRLQQLMRGDEGYLLALAYSTQRGYGRN
                     HPFAGEIRSGYIDIFIVPEELGFAVNVGELLMTECEMVNGFIDPPDEPPHFTRGYGLV
                     FGMSERKAMAMALVDRALQAPEYGEHATGPAQDEEFVLAHADNVEAAGFVSHLKLPHY
                     VDFQAELELLKRLQQEQNHG"
     gene            complement(15985..16569)
                     /gene="phnH"
                     /locus_tag="NBI74_002021"
     CDS             complement(15985..16569)
                     /gene="phnH"
                     /locus_tag="NBI74_002021"
                     /EC_number="2.7.8.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnH"
                     /protein_id="HCD1932364.1"
                     /translation="MTLETAFMLPVQDAQHSFRRLLKAMSEPGVIVALHQLKRGWQPL
                     NIATTSVLLTLADNDTPVWLAAPLSNDIVNQSLRFHTNASLVNQPEQATFAVTDEAIS
                     SEQLNALSTGTAVAPEAGATLILQVTSLSGGRMLRLTGAGIAEERMIAPQLPKCILHE
                     LTERPHPFPLGIDLILTCGERLLAIPRTTHVEVC"
     gene            complement(16566..17018)
                     /gene="phnG"
                     /locus_tag="NBI74_002022"
     CDS             complement(16566..17018)
                     /gene="phnG"
                     /locus_tag="NBI74_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnG"
                     /protein_id="HCD1932365.1"
                     /translation="MHADTATRQHWMSVLAHSQPAELAARLNALNINADYEVIRAAET
                     GLVQIQARMGGTGERFFAGDATLTRAAVRLTDGTLGYSWVLGRDKQHAERCALIDALM
                     QQNRHFQNLSETLIAPLDADRMARIAARQAEVNASRVDFFTMVRGDNA"
     gene            complement(17019..17744)
                     /gene="phnF"
                     /locus_tag="NBI74_002023"
     CDS             complement(17019..17744)
                     /gene="phnF"
                     /locus_tag="NBI74_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate metabolism transcriptional regulator
                     PhnF"
                     /protein_id="HCD1932366.1"
                     /translation="MHLSTHPTSYPTRYQEIAAKLEQELRQHYRCGDYLPAEQQLAAR
                     FEVNRHTLRRAIDQLVEKGWVQRRQGVGVLVLMRPFDYPLNAQARFSQNLLDQGSHPT
                     SEKLLSVLRPASGHVADALGITEGENVIHLRTLRRVNGVALCLIDHYFADLTLWPTLQ
                     RFDSGSLHDFLREQTGITLRRSQTRISARRAQAKECQRLEIPNLSPLLCVRTLNHRDG
                     ESSPAEYSVSLTRADMIEFTMEH"
     gene            complement(17765..18544)
                     /gene="phnE"
                     /locus_tag="NBI74_002024"
     CDS             complement(17765..18544)
                     /gene="phnE"
                     /locus_tag="NBI74_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313113.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HCD1932367.1"
                     /translation="MQTITIAPPKRSWFSLLSWAVVIAVLVVSWQGAEMAPLTLIKDG
                     GNMATFAADFFPPDFSQWQDYLTEMAVTLQIAVWGTALAVVLSIPFGLMSAENLVPWW
                     VYQPVRRLMDACRAINEMVFAMLFVVAVGLGPFAGVLALFIHTTGVLSKLLSEAVEAI
                     EPGPVEGIRATGANKLEEILYGVLPQVMPLLISYSLYRFESNVRSATVVGMVGAGGIG
                     VTLWEAIRGFQFQQTCALMVLIIVTVSLLDFLSQRLRKHFI"
     gene            complement(18649..19665)
                     /gene="phnD"
                     /locus_tag="NBI74_002025"
     CDS             complement(18649..19665)
                     /gene="phnD"
                     /locus_tag="NBI74_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter substrate-binding
                     protein"
                     /protein_id="HCD1932368.1"
                     /translation="MNAKIIASLAFTSMFSLSTLLNPAYAEEQEKALNFGIISTESQQ
                     NLKPQWTPFLQDMEKKLGVKVNAFFAPDYAGIIQGMRFNKVDIAWYGNLSAMEAVDRA
                     NGQVFAQTVAADGSPGYWSVLIVNKDSPINNLNDLLAKRKDLTFGNGDPNSTSGFLVP
                     GYYVFAKNNISASDFKRTVNAGHETNALAVANKQVDVATNNTENLDKLKTSAPEKLKE
                     LKVIWKSPLIPGDPIVWRKNLSETTKDKIYDFFMNYGKTPEEKAVLERLGWAPFRASS
                     DLQLVPIRQLALFKEMQSVKDNKGLNEQDKLAKTTAIQAQLDDLDRLNNALSAMSSVS
                     KAVQ"
     gene            complement(19690..20478)
                     /gene="phnC"
                     /locus_tag="NBI74_002026"
     CDS             complement(19690..20478)
                     /gene="phnC"
                     /locus_tag="NBI74_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HCD1932369.1"
                     /translation="MQTIIRVEKLAKTFNQHQALHAVDLNIHHGEMVALLGPSGSGKS
                     TLLRHLSGLITGDKSVGSHIELLGRTVQREGRLARDIRKSRANTGYIFQQFNLVNRLS
                     VLENVLIGALGSTPFWRTCFSYFTREQKQRALQALTRVGMVHFAHQRVSTLSGGQQQR
                     VAIARALMQQAKVILADEPIASLDPESARIVMDTLRDINQNDGITVVVTLHQVDYALR
                     YCERIVALRQGHVFYDGCSQQFDNERFDHLYRSINRVEENAKAA"
     gene            complement(20611..21054)
                     /gene="yjdN"
                     /locus_tag="NBI74_002027"
     CDS             complement(20611..21054)
                     /gene="yjdN"
                     /locus_tag="NBI74_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family metalloprotein YjdN"
                     /protein_id="HCD1932370.1"
                     /translation="MPLSPYLSFAGNCSDAIAYYQRTLGAELLYKISFGEMPKSTQDS
                     AENCPSGMQFPDTAIAHANVRIAGSDIMMSDAIPSGKASYSGFTLVLDSQQVEEGKRW
                     FDNLAANGKIEMAWQETFWAHGFGKVTDKFGVPWMINVIKQQPTQ"
     gene            complement(21214..21549)
                     /locus_tag="NBI74_002028"
     CDS             complement(21214..21549)
                     /locus_tag="NBI74_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phnA family protein"
                     /protein_id="HCD1932371.1"
                     /translation="MSLPHCPKCNSEYTYEDNGMYICPECAYEWNDAEPAQESDELIV
                     KDANGNLLADGDSVTIIKDLKVKGSSSMLKIGTKVKNIRLVEGDHNIDCKIDGFGPMK
                     LKSEFVKKN"
     gene            21952..24180
                     /gene="crfC"
                     /locus_tag="NBI74_002029"
     CDS             21952..24180
                     /gene="crfC"
                     /locus_tag="NBI74_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="clamp-binding protein CrfC"
                     /protein_id="HCD1932372.1"
                     /translation="MYTQTLYELSQEAERLLQLSRQQLQLLEKIPLSVPGDDAPQLAL
                     PWSQPNIAERHAMLNNELRKISRLEMVLAIVGTMKAGKSTTINAIVGTEVLPNRNRPM
                     TALPTLIRHTPGQKEPVLHFSHVAPIDHLIQQLQQRLRDCDIKHLTDVLEIDKDMRAL
                     MQRIENGIAFEKYYLGAQPIFHCLKSLNDLVRLAKALDVDFPFSAYAAIEHIPVIEVE
                     FVHLAGLESYPGQLTLLDTPGPNEAGQPHLQKMLNQQLARASAVLAVLDYTQLKSISD
                     EEVREAILAVGQSVPLYVLVNKFDQQDRNSDDADQVRALISGTLMKGCITPQQIFPVS
                     SMWGYLANRARHELANNGKLPAPEQQRWVEDFAHAALGRRWRHADLADLEHIRHAADQ
                     LWEDSLFAQPIQALLHAAYANASLYALRSAAHKLLNYAQQAREYLDFRAHGLNVACEQ
                     LRQNIHQVEESLQLLQLNQAQVSGEIKHEIELALTSANHFLRQQQDAVNAQLAALFQD
                     NSESLSEMRTRCETLLQTAQNTISRDFTLRFAELESTLCRVLTDVIRPIEQQVKMELS
                     ESGFRPGFHFPVFHGVVPHFNTRQLFSEVISRQEATDEQSTRLGVVREIFSRWLNQPD
                     WGRGNEKFPTETVDYSVLQRALSAEVDLYCQQMAKVMAEQVDESVTAGMNTFFAEFAS
                     CLTELQTRLRESMALRQQNESVVRLMQQQLQQTVMTHGWIYTDAQLLRDDIQTLFTAE
                     RY"
     gene            24177..25055
                     /locus_tag="NBI74_002030"
     CDS             24177..25055
                     /locus_tag="NBI74_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjcZ-like family protein"
                     /protein_id="HCD1932373.1"
                     /translation="MTKTLLDGPGRVLESVHPRFLVDLAQGDDARLPQAHQQQFRERL
                     MQELLARVQLQTWTNGGMLNAPLSLRLTLVEKLASMLDPGHLALTQIAQHLALLQKMD
                     HRQHSAFPELPQQIVALYEWFSARCRWKEKALTQRGLLVQAGEQSEQIFTRWRAGAYN
                     AWSLPGRCFIVLEELRWGAFGDACRLGSPQAVALLLGDLRVKATQHLAESINAAPTTR
                     HYYHQWFASSTVSTGGDHADFLSWLGKWSTADKQPVCWSVTQRWRTVALGMPRLCSAQ
                     RLAGAMVEEIFSVNLV"
     gene            25319..26821
                     /gene="proP"
                     /locus_tag="NBI74_002031"
     CDS             25319..26821
                     /gene="proP"
                     /locus_tag="NBI74_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline transporter ProP"
                     /protein_id="HCD1932374.1"
                     /translation="MLKRKKVKPITLRDVTIIDDGKLRKAITAASLGNAMEWFDFGVY
                     GFVAYALGKVFFPGADPSVQMVAALATFSVPFLIRPLGGLFFGMLGDKYGRQKILAIT
                     IVIMSISTFCIGLIPSYDTIGIWAPILLLICKMAQGFSVGGEYTGASIFVAEYSPDRK
                     RGFMGSWLDFGSIAGFVLGAGVVVLISTIVGEENFLDWGWRIPFFIALPLGIIGLYLR
                     HALEETPAFQQHVDKLEQGDREGLQDGPKVSFKEIATKHWRSLLTCIGLVIATNVTYY
                     MLLTYMPSYLSHNLHYSEDHGVLIIIAIMIGMLFVQPVMGLLSDRFGRRPFVLLGSVA
                     LFVLAIPAFILINSNVIGLIFAGLLMLAVILNCFTGVMASTLPAMFPTHIRYSALAAA
                     FNISVLVAGLTPTLAAWLVESSQNLMMPAYYLMVVAVVGLITGVTMKETANRPLKGAT
                     PAASDIQEAKEILVEHYDNIEQKIDDIDHEIADLQAKRTRLVQQHPRIDE"
     gene            26933..27022
                     /gene="pmrR"
                     /locus_tag="NBI74_002032"
     CDS             26933..27022
                     /gene="pmrR"
                     /locus_tag="NBI74_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000797656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LpxT activity modulator PmrR"
                     /protein_id="HCD1932375.1"
                     /translation="MKNRVYESLTTVFSVLVVSSFLYIWFATY"
     gene            complement(26998..28098)
                     /gene="pmrB"
                     /locus_tag="NBI74_002033"
     CDS             complement(26998..28098)
                     /gene="pmrB"
                     /locus_tag="NBI74_002033"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PmrB"
                     /protein_id="HCD1932376.1"
                     /translation="MNLMRFLRRPISLRQRLILTIGAILLVFELISVFWLWHESTEQI
                     QLFEQALRDNRNNDRHIMREIREAVASLIVPGVFMVSLTLFICYQAVRRITRPLAELQ
                     KELEARTADNLTPIAIHSATLEIDAVVSALNDLVSRLTSTLDNERLFTADVAHELRTP
                     LAGVRLHLELLAKTHHIDVAPLVARLDQMMESVSQLLQLARAGQSFSSGNYQHVKLLE
                     DVILPSYDELSTMLDQRQQTLLLPESAADITVQGDATLLRMLLRNLVENAHRYSPQGS
                     NIMIKLQEDGGAVMAVEDEGPGIDESKCGELSKAFVRMDSRYGGIGLGLSIVSRITQL
                     HHGQFFLQNRQETSGTRAWIRLKKDQYVANQI"
     gene            complement(28099..28767)
                     /gene="basR"
                     /locus_tag="NBI74_002034"
     CDS             complement(28099..28767)
                     /gene="basR"
                     /locus_tag="NBI74_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BasR"
                     /protein_id="HCD1932377.1"
                     /translation="MKILIVEDDTLLLQGLILAAQTEGYACDGVSTARMAEQSLEAGH
                     YSLVVLDLGLPDEDGLHFLARIRQKKYTLPVLILTARDTLTDKIAGLDVGADDYLVKP
                     FALEELHARIRALLRRHNNQGESELIVGNLTLNMGRRQVWMGGEELILTPKEYALLSR
                     LMLKAGSPVHREILYNDIYNWDNEPSTNTLEVHIHNLRDKVGKARIRTVRGFGYMLVA
                     NEEN"
     gene            complement(28764..30407)
                     /gene="eptA"
                     /locus_tag="NBI74_002035"
     CDS             complement(28764..30407)
                     /gene="eptA"
                     /locus_tag="NBI74_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase EptA"
                     /protein_id="HCD1932378.1"
                     /translation="MLKRLLKRPSLNLFSWLLLATFYISVCLNIAFFKQVLQVLPLDS
                     LHNVLVFLSMPVVAFSVINIVLTLGSFLWLNRPLACLFILVGAAAQYFIMTYGIVIDR
                     SMITNIIDTTPAESYALMTPRMLLTLGLSGVLAAIIACWIKIKPTTSRLRSVLFRGAN
                     ILISVLLILLVAALFYKDYASLFRNNKELVKSLSPSNSIVASWSWYSHQRSANLPLIR
                     IGEDAHRNPLMQNGKRKNLTILIVGETSRAENFSLNGYPRETNPRLAKDNVVYFPNTA
                     SCGTATAVSVPCMFSDMPREHYKEELAQHQEGVLDIIQRAGINVLWNDNDGGCKGVCD
                     RVPHQNITALNLPGQCINGECYDEVLFHGLEDYINNLQGDGVIVLHTIGSHGPTYYNR
                     YPPQFRKFTPTCDTNEIQTCTQEQLVNTYDNTLVYVDYIVDKAINLLKEHQDKFTTSL
                     VYLSDHGESLGENGIYLHGLPYAIAPDSQKQVPMLLWLSEDYQKRYQVDQNCLQKQAQ
                     TQHYSQDNLFSTLLGLTGVETKYYQAADDILQTCRRVSE"
     gene            complement(30511..31848)
                     /gene="adiC"
                     /locus_tag="NBI74_002036"
     CDS             complement(30511..31848)
                     /gene="adiC"
                     /locus_tag="NBI74_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine/agmatine antiporter"
                     /protein_id="HCD1932379.1"
                     /translation="MSSDADAHKVGLIPVTLMVSGNIMGSGVFLLPANLASTGGIAIY
                     GWLVTIIGALGLSMVYAKMSFLDPSPGGSYAYARRCFGPFLGYQTNVLYWLACWIGNI
                     AMVVIGVGYLSYFFPILKDPLVLTITCVVVLWIFVLLNIVGPKMITRVQAVATVLALI
                     PIVGIAVFGWFWFRGETYMAAWNVSGLGTFGAIQSTLNVTLWSFIGVESASVAAGVVK
                     NPKRNVPIATIGGVLIAAVCYVLSTTAIMGMIPNAALRVSASPFGDAARMALGDTAGA
                     IVSFCAAAGCLGSLGGWTLLAGQTAKAAADDGLFPPIFARVNKAGTPVAGLIIVGILM
                     TIFQLSSISPNATKEFGLVSSVSVIFTLVPYLYTCAALLLLGHGHFGKARPAYLAVTT
                     IAFLYCIWAVVGSGAKEVMWSFVTLMVITAMYALNYNRLHKNPYPLDAPISKD"
     gene            complement(31985..32746)
                     /gene="adiY"
                     /locus_tag="NBI74_002037"
     CDS             complement(31985..32746)
                     /gene="adiY"
                     /locus_tag="NBI74_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator AdiY"
                     /protein_id="HCD1932380.1"
                     /translation="MRICSDQPCIVLLTEKDVWIRVNGKEPISLKANHMALLNCENNI
                     IDVSSLNNTLVAHISHDIIKDYLRFLNKDLSQIPVWQRSATPILTLPCLTPDVFRVAA
                     QHSMMPAETESEKERTRALLFTVLSRFLDSKKFLSLMMYMLRNCVSDSVYQIIESDIH
                     KDWNLSMVASCLCLSPSLLKKKLKSENTSYSQIITTCRMRYAVNELMMDGKNISQVSQ
                     SCGYNSTSYFISVFKDFYGMTPLHYVSQHRERTVA"
BASE COUNT         7493 a         8474 c         8874 g         8010 t
ORIGIN      
        1 taaatacttt cgggagcgtt tacgccccca aaagcgagtg atattaattt ggggttacga
       61 tattcaccca caaatcgaaa ttatccagac aggcgataat ttcttccagt ttcgcactat
      121 tgccaataat tttggctttc ttcgctttca ccagatcggc catttttgcc tgtccggtca
      181 gcacggcgtg cagatcttca cggctgatgt agaaagaggc gtcagcttgc ggttgcagtg
      241 ttttacggta gttaagcacg ctatcgttca gcgtcaggtt gaggttatcg ccgttgctca
      301 tattgaagtt caggctgata tttttacccg cggctttcgc gctatcgagg cgaacggcca
      361 taaagtcgaa cagcatttcg actgacatcc cacgaagggt gtccggggaa ccggtggtgc
      421 cgtggctgaa cttatgcacc ccttcgcgca gctctttcgc gccggtcagg tagaaaccgc
      481 gccatgtggc ggactcggcc tgatagccca gctgttcaaa gttattcgct tgcagattct
      541 tcgcgacctg gtcacccggg ttggcggcaa tcacctgttt cagaagttct gccgaccagc
      601 ggtaatcacc ttgcttgttc gcttcttgcg ccaggttgat gacgcgggca gaaccgccca
      661 gcgcctgcac gtaacgttta cccatctcca cctgaccata gggatgcagg ttagccgggt
      721 taccgtcgta atagccaaga tagaagttat ataccgcgcg ggcgttgtgg ctgacagagc
      781 cgtaatagcc gcggctggcc cagttattgg aaagcgcagg cggcagctta atcatgtcgc
      841 cgatttcatt catggtgtag ccctggttcg ccaggtgcag ggtctggtcg tgaatgtact
      901 tgatagtatc gcgatattta ccaatataat cattgatatg cttattgccc cagaccggcc
      961 aggtgtgcgg catgaacagt acttccgcgt cgttacccca catatccaac gtttcgttca
     1021 gatactcggt ccacttgctg gtgtcgcggg ttttcgcgcc gcgcagggtg tagaagttgt
     1081 gcaaggtgtg agtggcattc tcggcggtac acagggcttt cagggccgga atatagaagt
     1141 gcatttcggc tggcgcttcg ctacctgggg tcatcaggaa gtcaaactcc aggccgtcga
     1201 taatcatctt ctcgccagtt ctcacgatag ttttagtcgg tgcaataatg cttgggtcgc
     1261 cggttgccag cgtcacgccg aggccgttgc ccacgttgcc ttgcgcgttg tgcggcagta
     1321 acagaccgta agagtagagc gcacggcggc tcatgatgtt acccgccagc acgttttcgc
     1381 tgatggcttc gtccataaag ccagctggag caatcacctg aactttgccg gatttaacat
     1441 cggcttcaga gataatacct ttcacgccgc cgtagtggtc ggcgtggctg tgagtgtaga
     1501 taacggcaac aatcggtttt tgcggacgat gctggaagta aaggtcaagt gcggcttttg
     1561 cggcaggtgg cgtcaccagt gggtcaataa caataatgcc tttctcgccc tcaacgaacg
     1621 taatgttaga gatatcctgg ccgcgcacct gatacatttt atcggtgact ttgaacaggc
     1681 ctgaaatgcc gttgatctgc gactgacgcc acaggctggg gtttacggtt tccggcgcag
     1741 cggcattaat atcaaatttg taatcattcg cgcggtagta aattttaccg ttcgcatcac
     1801 gcagaatacc ttcatccagc agcggagcga taaatccacg cagagcatcg tcaaaatcct
     1861 ggcgatcgct aaacggtaat gatttggcgt aattctgatt aacctgttgg gtatattgag
     1921 tggcagcttt cgcttcatct gttgcgtaag cggtatttat catcatgccg ctggcggcag
     1981 tgaacgcaat aacaatcctg ctcaaacgga ataaccgaga gttattcata taaatctcca
     2041 tttatcattt atgtcgtaaa tatgtaatcc atgcaaaata cgtcatttaa agctggcgtt
     2101 atcataattc tgaaattgcg actttaataa gtggaagtgt gagcggaacg atccatttta
     2161 ttaggcattt gtgctgaaga gggaaaatta cagtagaact atcattgctg ggaataatat
     2221 tggctgtttt atgtaattat tatatatatt gtacctgtcg ttggcacagc gggacattgc
     2281 gctactgtgc caacgcttta ctcatgggac tttagcacag gactgtggca aaaaacgttg
     2341 atgcgccaat attgctgcac cctgagcgcc attaaagtca gaagatgagg cggcaataaa
     2401 gcgcacgacc tgatacggca gtggacggcg caggtacttt tgggtcatgg caatgagagt
     2461 ctcgcgtggg aaggcgggca tatccatcac gccaccaccc agaatcaccg catcgggatc
     2521 gaacagatta atgctggtgg caatggctcg tgccgcgttt tcaagcagac tctggacgaa
     2581 aggggcgttt tccgcatgga cgaaaagatc gctcaatggg taatttcggg actgttgttc
     2641 gtaccagcgt cttagcgcca ttccagagca attggtttcc aggcacccag gattaccaca
     2701 cgcgcagtgt tgggtcatat ctcccagggg gatatggccc agttcgcctg ccacaccgtg
     2761 tgcacccgtc cacggcgcac cgttcatcca cactgcgaac cccatccccg taccgagata
     2821 ggctgccaga accagttgtt gcgtaaggcg gttttctact acgtcccagg agagttgcag
     2881 gttaacgtcg cgggaaaact caaccggaca attcagcgta ttttcgagct tatcggcgag
     2941 atcatataaa tctgccgctg ttaacggcag gttaggcgta gaaataatgg tgcgtttatc
     3001 tttactgacc agcgccggaa atcccatcac cagaccatga cagcgagcgt taaagcgcct
     3061 gagttgctcg tcaatcattt cgccgatacc cgacaccagg tcgggagcaa taacttctgc
     3121 ggtccgcttt ttttcgcagt gtagcgtttc accttctgct gtccgcagac aaaagcggat
     3181 atgcgttgcc cccatatcca cgcccgctac gacgttatgc tgtttttgca tgaggctgta
     3241 cctcgctttt tgcagccaga atctgcgcgg tcataattct ccatgcttcg tcgatatttt
     3301 ccgcatgatt aaacaggccg gaagtgccga cgataaagac atccgcccct gccgccatta
     3361 gtttttcgta agttgcctgg ttgcaggaac cgtccacctc aatttcgtac tccagacctt
     3421 ctcgttcacg ccatgccttc agttcggcaa ctttatccag catttcagga atgaagggtt
     3481 gtccggcaaa accgggatcg acagtcatga ccgtaatttt atcagcctta tggatatagt
     3541 atttcatggc ctcaaccggc gtttccggat taagaatcag ccccactttc atgccatgac
     3601 ggcggatttc atcaatcagg cggaatgcct gaccgttgat agtttccgga tgcaaagtga
     3661 tgaaatcagc tcccgcacgt gccagttgag caatgtaatc ctgcggacgc gttaccatca
     3721 gatggcagtc gagcggttta ctcgccagtt tttttacctg gcttacgaag aacggtgaga
     3781 gcgtcagatt cggcacaaag tggccgtcca tgatatcgat gtgaaagtaa tcggcatggc
     3841 tgtcgataaa ttcgatctgt tctttaaatt tcagcagatc catacacatt aacgaggggg
     3901 agattttcat gttcaattcc ttacttactg ataagacggt caagggcgac agccgcgata
     3961 attaatccgc ccatcaccac cagttggtaa taggtttgta cctgcaaaat attcagaccg
     4021 ttgttgatgg tgccgatgat caacccgcca atcaccacag agaaaatgcg ccccttgccg
     4081 ccgaagaaac tggtgccgcc aatgatggcg ctggcaatgg cataggtttc aaaacccata
     4141 ccggcaagcg gttctgcggc accgagtcgt gcagttgaga cgacgcctgc cagacctgca
     4201 caaacaccgg agatgataaa caccaccagg atgtggaatt tcacgtcaat cccggaatag
     4261 aacgccgagt ttttgttgcc gcccagtgcg tagatgttgc gtccgagccg catacgcgtt
     4321 gtcagaaacc aaaggatgag cgcgacaatt agcgagaaga taacggggac aggtatccca
     4381 attacgctgg cggcaaagaa gttcacgaag tcaaatgaga agccgtatac cgagttggca
     4441 tcggagatca ccagcgtgat cccacggaaa atagcgttgg tgccaagggt gataatgaac
     4501 gggtgtagtc ccgtccagtt gaccaggcag ccgttgatcg cccccagtgc gccgccaacc
     4561 agtacaccgc caattagcgc cgcgagaaac ggatcaatac ctgccaacat cagtttcgcg
     4621 gtcaccatac cggaaagcgc cagaatcgcg ccaaccgaaa ggtcgatacc ggcgaccagg
     4681 atagcgaaga attcccccat gccgatcagt accgtcacgg agctttgcac aaaaatctgg
     4741 gtaatattat tggtggtcag aaaatattct ggcgacagcg aaccaaaaat ggcgacgatg
     4801 atcgccagga taaaaaaggt gccgtattta tcccagaaca gcgcaaagtt gaacggtttc
     4861 ttctcgctcg cttcgctttt tactcttgtg gtaaagccca tgccataatc tcctcttcgc
     4921 tcatgtcatc gcgattggtc aggatttgcg tcagtcgtcc ttcgcagaac acggcgatgc
     4981 ggtcgcagac ggtgataatt tcaggtagtt cagatgacac catcaggatg acttttccgt
     5041 cgtccgccag ttggcgcatc actttgtaaa tttcggcttt cgcgccaaca tcgatgccac
     5101 gggtaggttc atcgaaaatg atcacttccg ggcgacagca cagccatttg gagatcaggg
     5161 ctttctgctg gttaccgccg gagagttcag tgatgttctg gtttaccgaa tgacatttca
     5221 gcgccagcag ttcgcgctgg gcttcagcgg tacgttgctc gtcaacttca tgaaacaacc
     5281 ccatcgcgcc tttatagccg ccgtctttca gactgcggct gatagccatg ttctgagcga
     5341 tagaaaagtt ggggaaaaag ccgttatccc ggcggctttc agtgatgtaa gccatccctt
     5401 ttttcacggc atccagggga gaacgtggag agatatcttt gccattaaga cggatttctc
     5461 cgccagcgcg tttatcaacg ccaaacaggc agttcatcag ttcagtacgt ccggaaccga
     5521 ccagtccggc aaagcctaat atttctcccc ggcagacgct aaatgagata tcccggacct
     5581 tttttctgtc acggctggtg acgttgcgca cctcaaaaac cgtatcgtgc gcaaggttgc
     5641 tgacattctc cttcatcgcg ttaaaacggt tttgcagttc gcggcctacc atcagacgga
     5701 cgatatcgtc atttgacaca tcgctcacca tgccgctgca aacgctgctg ccgtctttca
     5761 tcaccgtata gcggtcgcaa atacggcgaa tttccgccaa tttatgcgag atatagacga
     5821 tggccgtacc ctccttacgc aactgattca tgatcagaaa cagatagtcc acctctttat
     5881 tggtgagtga ggaggtgggt tcatccatga tgatgacttt ggcatcgagc atcagcgttt
     5941 tggcaatttc cagcatctgc ttgtggctga tagataagtt cgccactttc tcatctaaat
     6001 caactttcaa gcccacgcgt aataacatca tggcggcgcg gacacgcatt tctcgccagt
     6061 cgataatatt gacgccacag atttttttcg tcagatgacg accaatatat aaattttcca
     6121 gtacggttaa ttcatcaata acgctgagtt cctgataaat aatcccgata ccgagttgtg
     6181 ccgctaattt atgatccagt ttgttatagt taatgttatt aatggtaatg gtgcctttgg
     6241 tcggctcatg tattccggat aacactttca ttagcgtaga tttacctgca ccattttctc
     6301 ctagtaatgc atgtatttca ctaggataaa ccgttaaatt aaccgacttt aatgcgtgaa
     6361 ccggaccaaa ggacttgccg atccccgcca tcgatatata tggcgtggcc ataaataacc
     6421 tctctgcacg aaatatcgta ttattgcctg atggctacgt ttatcaggtc tacgttcagg
     6481 ttattttgtt ggccggataa gacacttcat tagcatcatc atccggcatc attcagtttt
     6541 attgagtgac caggattgaa tcgaccagtt taaattccgg tgctttatcc agcgggataa
     6601 ccttgccgga tttctcagcg tcaaccatca gcttcagacc cgttgcaccg atatccgccg
     6661 ggttctgggc aaccgtcgcg gtcatttgtc cggcttccac cattttgcgg gcttccggaa
     6721 tgccatctgt accgacgacc aacactttgc ccgtttttcc agcgtttgcg actgcctgag
     6781 caacacccat tgccatcgtg tcattcgcgc aatagatcgc tttaatattc ggattacgtt
     6841 gcaacacgtt agtggcgaca tccagtgctt taatacggtc ccagtcggca ggctgactgg
     6901 cgacaagctt gatctggctt gcttttttga aggcttcggt ggcgccatta cgacgcgctt
     6961 caccggaggc gttaccggct ttaccctcaa tgattgcgac ttcaccccct tcggcgccca
     7021 atttgtcaat aatgaatgac gcgcctttcg ccccgacagc aacattatcg gtggtgacaa
     7081 aaccttccac attaccgcct gcttttttca gattatccat gtcgattttt tcatcgagat
     7141 tgaccagata aatgcctttt ttccatgcgc gggcgacagg catgaccaga tttactgagg
     7201 ataatggcgc gaaggcgata cctttgtaat ttttattact gagatcttca aataactgca
     7261 attgagattg aaaatcgcct tctgaaggag aggcaaaaat atcaacgctg acgcccagcg
     7321 tttttgcttc atcttcaatg ccttttttca tatctaccca aaatgggttg gagagtgttt
     7381 tcaatacgac ggcatattcg gcggcagcaa aagcgctggt tgacaacatt aagcccacga
     7441 gtgtgccgct gaaatatttc agatatttat tcataatcat gttctcacgg taaggggata
     7501 ggtaactccg cgatggcgga attacctttt tcttatttca gcgcgcctgg tgagaagaaa
     7561 tcaacaattg cgccggtttt ttgcatatta atattagctt gttcaatatt gagctgggcg
     7621 acagagacaa aaaacgcgtc cagcaaagtc agttgtaata ttcttgccga ggcattacga
     7681 cctaataacg gtgtttccgg ggccggtgag caaataatat aatcggccag cttcgctatt
     7741 ggtgaatgat agctatgcgt tatacaaata atctttgccc cgttcttttt tgccagttct
     7801 acagccgctt ttacatcact ggttcgcccg gaatgggtca ccaccagcac cacatctcct
     7861 tcctgtaaca acgaagcaga catcatcatg atgtgagcgt ccggataggc ctggcagcgt
     7921 acgccaatgc gcaaaaactt gtgctgtaca tcagcacaga tagcatttga tcctccggca
     7981 ccgtacaaat cccgctgtct ggcctgatag aaaaagcggg cggcacggtg gatctcatca
     8041 acgttgacga tcgactgacc ttccataatg gtgcgtaaag tgatgttaaa taccttattc
     8101 accacatcct gcggcgcttc atcaaaagcc aactcggacg gcaatacttg ttctgactga
     8161 gaaaaataat cttccagcgc actgcgtaag ttacgaaagc cgctaaaccc cagcagcttt
     8221 gataccttaa cgatcatcgc ttcagatacc gccagagctt ctgcgacatc tttaattgcg
     8281 ggtgcacaac tcaggttacc gggtttgagt aaccactcca cgatgcggct ttcgttttct
     8341 gtcattcctt cctgcttcat tcgcaggtaa ggcgctaacc cgataccgtt cggaagcgct
     8401 gaatcaaact ctgactggct catcacgttc tcttgtcctt gaattgaaga cgccactata
     8461 tcaccacagc aagaacagaa aagatgctgg caacttcaca aaaaaataaa atttataaag
     8521 tttgttttat tgtgaataat ggagagtggc ttaaaatttt atttcattta atcactttaa
     8581 aaacatgatg ttatttgttt ttctggttta ttagatgata gatgttgccg gatagtgagt
     8641 gtctttaacg ctattgcgct gccagatgta actctgtaaa acagatcggg aaggctgatt
     8701 tcatcgattt tgggtgtttt taattcattt aaatcacaaa tgttttttta ttgtgaagtt
     8761 ttgcgcgagc ggggaagatg aatgaaaaag attgcatttg gctgtgatca tgtcggtttc
     8821 attttaaaac atgaaatagt ggcacattta gttgagcgtg gcgttgaagt gattgataaa
     8881 ggaacctggt cgtcagagcg tactgattat ccgcattacg ccagtcaagt cgcactggcc
     8941 gttgctggcg gagaggctga tggcgggatt ttgatttgtg gcactggcgt cggtatttcg
     9001 atagcggcga acaagtttgc cggaattcgc gcggtcgttt gtagcgaacc ttattccgcg
     9061 caactttcgc ggcagcataa cgacaccaac gtgctggctt ttggttcacg agtagttggc
     9121 ctcgaactgg caaaaatgat tgtcgatgcg tggctgggcg cacagtacga aggcggtcgt
     9181 catcaacaac gcgtggaggc gattacggca atagagcagc ggagaaattg agattcatcc
     9241 actacttgca tggatgagta atgactaatg tggatagagt ttctttttga ggttggctaa
     9301 tgaaacgctt tccgctattt cttttattca ccctgttcac gctgttcacg ctgttcaccg
     9361 ttccggccca ggccgatatt atcgatgaca ccattggcaa tattcagcag gcgattaacg
     9421 acgcttataa ccctgaccgt gggcgagact atgaagattc gcgcgacgac ggctggcagc
     9481 gtgaagtgag cgacgatcgg cgcagacaat atgatgaccg ccgccgccag tttgaagatc
     9541 gccgcaggca gctggacgaa cgccagcgcc agcttgatca ggagcgtcag caactggaag
     9601 atgaagagcg gagaatggaa gatgagtatg ggcgttgagg taggggccgg cgcgggtatt
     9661 ttccctcact ccacctcaat ctccatccca tcaaaccccg cctcaaaccc cgacggcagt
     9721 gcattttcca tcagccacgc atcaaactgg tggctgatat gggtcagaat cacccgtggc
     9781 gagcggataa cctgattcag cgcaagcacg gtatttaaat cacagtgatt acgcggtgca
     9841 tcctcgcgcg gcgggtggct gcaatcaatc accattacct gcggctgatt attgcgtaaa
     9901 aatttcagcg ttttttccgg caggcctgcg gtgtcagata gccacgccac ccggctgtgc
     9961 gccgtttcca gtaaatagcc gaaagtgagt ttcgaatggt taagcggcag cggcgtgacc
    10021 tgcaacccct gcaaatcaaa caccacaaac ggctccaccg tgtggctgaa atcaagcagg
    10081 cccggatgtt taaacagatc gtcgcagccc tgctcatccg gtggaccgta gaccgggatt
    10141 gtgtcgccca cgccccagcg cagcggaaac agcccctgca cgtgatccat atggtagtgc
    10201 gtcagcaaaa actgctggaa cgatccgggc gaccagcgat cggcgagatc gtgcagcccg
    10261 gcgtcgatca gggtgatcgc gtcgttaaac ttcacgacgc cgctgcatgg ctggcggcga
    10321 tactgcggtg agcgccgcgc tctggcgcag gccgcgcact cacagcccca tgcgggaacg
    10381 ccctgtgcgc cgccagtgcc ggtgagcgtg aggatcaggc tcatgttaca gcgctttggt
    10441 gaagcggaaa tggctctgct cgtagccttc gcgcagatag aaacggtgcg cgtcgtggcg
    10501 cttcacgttg gtcgaaagct cggtcatttc ggccccggcc tggcgggctt cttcttctgc
    10561 ccacgccagc aacttgctac cgacgttcag gccgcgcgcc tgcggcatca ccaccaactc
    10621 ctgaatttcg ccgatccagt tgacatgatg cagatgaaac tgcaaatgca ggccgatcat
    10681 gccgacaact tcgccatcca gcagcgccag atggtagcgc atgttcggat cgcgcagatt
    10741 ggcgttaaac cccacgcgaa acgcgtggtg gtcaaactcc gcctgtttta gctcacaaat
    10801 cagcgcgtaa actgcgtcgg tgtcgtactg cgtagccggg cgaagctcac aagcaggcat
    10861 ggtgtttctc cttctgatga atcagcgtca gcagcttgtc gaccgactgg cgcaggctgc
    10921 cgtcattatt gagcgtaaga caatcctgag gagtatagcg ggcagcgcgc gccagtcggg
    10981 cgttaatttc actggcattt tcacgaccac ggttttcgag gcgctggcgc aggatctccg
    11041 gcgaaacctg taaacaaacg ggcagcagcg ccgattgata gcgcgcccgc gcctgcggca
    11101 gatgggcgcg tgagccgttg accatcacgt caaatccagc gtgcagccag aggtcaatct
    11161 cgacgccgac gccataatac aggccgttag cgtgccagct taaggccagc agattttgcc
    11221 ccgcgcgggt aaaaaactcc tgctcgctca gggcgatatg gttttcactt ccggcgctgg
    11281 cggcgcgcgt gatgtagcga tgcgccacca gtaactgagt ttgttcccgc tggcggagtt
    11341 ccgtcaacag actgtctttt ccggagccag aggcccccat taaccaaatc aattttcccg
    11401 tcatcagaac acccttttac cctgacgcca gacgtggtcg atatgaatgt gattgccctt
    11461 gcgatgcgcc agcaccaggt cggcgcgttt gccctcgcca atcaccccgc gatcctggag
    11521 attaagcgcc tgcgccggat ttttagtcac cagcttcacc gcctgcggca gcgtaaagcg
    11581 gttgctctcg tcatcggcga cgcgaaatgc cgcatcgagc aggctggcgg ggtagtagtc
    11641 ggaagagagg atatccagca ggccaagctg cgccagttca ctggccgcca cgttgccgga
    11701 gtgcgaaccg ccgcgcacga tattcggcgc gcccatcagc acgttcatgc catgcttgcg
    11761 cgaggcttct gccgcttcga acgtggtggg aaattcggcg atcacgctgc caagctggtg
    11821 agattcggca acgtgggcgt gcgtggcgtc atcgtggctg gcgagggcaa tgtgtcgcgc
    11881 gcggcacatt gcggcgatgg attcgcgatt cggctgtgac cagcgtgcgg cgagcgccag
    11941 ttgctcctct tcgtactgtt gcatctgcgc atcggtgagc gagtatttgc cctgataata
    12001 ttcacgatat ttttcgcggt tggcgaactg gcgctggccc ggtgagtgat ccatcagcga
    12061 caccagtgtt accggttcgc gctgtaccag tttttcaaac agcggcagcg tggtgtgatg
    12121 cggcagttcg cagcgtaggt gcagacggtg ctcggcacgg ttgacgccgc gtttctgcgt
    12181 ctcttcgatg gcgttgatca tcttctccag attctccagc cgatcgccgc cgtcgcgtac
    12241 atcaccaatc gccacggcgt ccagcacggt ggtgatgccg ctcgccacca tcagcgcgtc
    12301 gtggctgctc atcgccgagt gggcaggcca gtcaactttc gggcgcgggg tgaagaattt
    12361 atccagatta tcggtatgca gctcaatcag ccccggtagc agccagccgc cttcgccgtc
    12421 cattgcttct gacaggtgac tctggctttc ggcaaaggcg cggatttcgc cgtcctgcat
    12481 ctccagagaa ccgtgtacca cctcgttttc cagcaccagt ttgacgttat tgataatcat
    12541 gaagagactc ccattgggtg caggcggtcg gcgacttgct cgcgtacggt ttcgtcatgg
    12601 aagataccga cgatggccgc gccgcgggct ttggcttcgc ggatcagttc cactacggcg
    12661 gcgctgtttt tagcgtccag cgaggcggta ggttcgtcga gcagcagaat ggggtagtcg
    12721 acgataaagc cacgggcgat gttgacacgc tgctgttcgc caccggagaa ggtcgatggc
    12781 gcaaggtgcc acaggcgttc aggcacgttc aggcgggtga ggagacgcgc ggctttagcg
    12841 gcgcaggctt cacgcggtac gccggtatcg agcagcggct gcatcaccac ttccagtgcc
    12901 gaaatacgtg ggatgacgcg cagaaactgg cttacccagc cgacagtggt tttgcggatc
    12961 tccaccactt tgcgcgctgg cgcggtgacc aggtcgaccc attcatcacc gtgtttgatc
    13021 tggatttgcc cttcgtcggg tagatagttg gcatacagcg agcgtagcag agttgatttt
    13081 ccactgccgg aatggccgtg gagtaccacg cattcgccgg cgttgacggt gagtgaggcg
    13141 ccttgtaata caggcaggcg cacgccgttt tgctggtgca ggatgaaggt tttactgacg
    13201 ttttgtacgt taatcatttt tcgcctcgtg gttcgggtgt cggatgcgac gctgatgcgt
    13261 cttatccgac ctacggggag cgcatttgta ggccggataa ggcgtttacg ccgcatccgg
    13321 caccagctca attctgcaaa accgatgaca ccagcaactg ggtatacgga tggtgcgggt
    13381 cgtcgagcac gcggtcggtt aacccactct ccaccacttt cccttgcttc attaccagca
    13441 aacggtccgc cagcagacgg gcgacgccta aatcgtgggt gacaatcacc accgccaggt
    13501 taagctccat caccagaccg cggagcaggt ccagcaggcg cgcctgtacc gacacatcca
    13561 gcccgccagt gggttcatcc ataaatacca gcttcggatg cgtcaccagg ttgcgggcaa
    13621 tctgcaaacg ttgctgcatg ccgccggaaa aggtggtcgg caggtcgtcg atccggttgg
    13681 cgggaatctc cacctcttcc agccatttct gcgcggtggc gcgaatatcg ccgtaatgac
    13741 gcgctccggt tgccatcaac cgttcgccga tattgccgcc tgccgacacc tggcggcgca
    13801 ggccgtcaag cggatgctga tgcaccacac cccattcggt gcgcagcagg cgacggcggt
    13861 cggcctcgct catcgcgtac agcgaacggt tctcgtagcg aatttcccct tgctgtggtg
    13921 tcaggcgggc ggaaattgat ttcagtagcg tggtcttccc ggaaccggac tccccaacaa
    13981 tgcccagaac ttctcccggc cataaatcaa aagagacatc gctgaagcct ttgcccgggg
    14041 cataaaggtg ggtcaggtta ttgaccgaaa gtaacggttg attcattggc ttttggcctc
    14101 gctctgttgg cggcaataat cggtatcgga gcagacaaac atgcggtttc cggcgtcatc
    14161 cagcaccact tcatcaagat agctgtgggt cgatccgcag atggcgcatg gttcatccca
    14221 ctgctggacg gtgaacggat gatcatcgaa atcgaggctt tccacgcggg taaacggtgg
    14281 cacggcatag atgcgcttct cgcgccccgc gccaaacagt tgcagggcgg gcatcatgtc
    14341 cattttcggg ttatcgaatt tcgggatcgg cgacgggtcc atcacgtagc gcccgttcac
    14401 cttcaccgga taggcgtagg tggtggcgat atgaccgaag cgggcgatat cttcatacag
    14461 cttcacctgc atcacgccgt actcctccag cgcgtgcatg gtgcgcgttt ccgtctcacg
    14521 cggctcaata aagcgcagcg gttcggggat cggcacctgg aagataacta tctgatcttc
    14581 ggtcagcggc gtttcgggta tgcggtgacg cgtctggata agtgtcgcat cgtccgtgcg
    14641 ctcggtggtg ttcaccccgg tcacgcgctt aaagaagtta cgaatcgaca cggcgttggt
    14701 ggtgtcgtct gcgccctggt caatcacctt caacacgtcg ctttcgccaa tcacgctggc
    14761 ggtgagctga atgccgccgg ttccccagcc gtagggcatc ggcatctcgc gtccgccaaa
    14821 cggcacctga taaccgggga ttgccaccgc ttttaagata gcgcggcgga tcatgcgttt
    14881 ggtctgctcg tcgaggtaag caaagttgta gccgctcaga ttagccatgg ttctgctcct
    14941 gttgcagacg tttgagtagc tccagttcgg cctggaaatc gacgtagtgc gggagtttga
    15001 gatgcgaaac aaagcctgcg gcttcgacgt tgtcggcatg ggccagcacg aactcttcat
    15061 cctgtgccgg acctgttgcg tgctcgccgt attccggtgc ctgcaacgca cggtcaacca
    15121 gcgccatcgc cattgctttg cgctcgctca tgccgaacac cagcccatag ccgcgtgtaa
    15181 aatgcggtgg ttcatccggc gggtcgataa aaccgttgac catttcacac tcggtcatca
    15241 acaattcgcc gacgttcacc gcaaatccca gctcttccgg cacaataaat atatcgatat
    15301 agccactgcg aatttcgcct gcgaacgggt gattgcgccc gtaaccgcgt tgggtggagt
    15361 aggccagcgc caacaaatag ccttcgtcac cgcgcattaa ttgttgtaga cgggaagaac
    15421 gcgaacaggg ataaaccggc ggcgtgcggg tgatgtcgtc cggttgtgcg ccgctatctt
    15481 cttcaaactt cgccagcccc tgacgcgcca gcaggctgaa gacgtgtggc gacggttgct
    15541 gttcgctgtc agcggtggtc agcgttggcg tttcgccgtt tgccagcagg gtgaaatcga
    15601 gcaggcgatg ggtgtagtcg taggtggggc caagcagttg gccgccggga atgtctttat
    15661 aaattgctga aatacgccgt tcgagacgca tcccggtggt gtcgagcggc tcactgaccg
    15721 ccagcttcgc cagcgtggtg cggtaggcgc gcaacaggaa aatcgcttcg acgttatcgc
    15781 cgctggcctg tttcagcgcc agcgcggcca gctcgcggtc ggcaataccg ccttcggtca
    15841 tcacgcgatc taccgcgagg ttaagctgct gttcaatctg ggcgacgctc agttcaggca
    15901 aatcagtatc gccccggcgt cggctctctt gcagggcgtg ggcggcgtcg atcgcctttt
    15961 cgcccccttt cacggcaacg tacatcagca cacctccaca tgagtggttc gcggaatagc
    16021 cagcagacgc tcgccgcagg tcaggatcag gtcgatgccg agcgggaacg ggtgcgggcg
    16081 ctcggtaagt tcatgaagaa tgcactttgg cagttgcggg gcgatcatcc gttcttcggc
    16141 aatacctgcg ccggtgaggc gcaacatacg cccgccgctc aggctggtga cctgtaaaat
    16201 cagcgttgcg cccgcttctg gtgcaacggc agtgccggtg gaaagggcgt tgagctgctc
    16261 gctggagatc gcctcatctg ttaccgcgaa ggtcgcctgt tccggctgat taaccagcga
    16321 cgcgttggta tgaaaacgca ggctctgatt gacgatatcg ttgctcaatg gcgcggcaag
    16381 ccacaccggc gtgtcgttat cggccagcgt cagcagcacg ctggtggtgg cgatattcag
    16441 cggctgccag ccgcgcttga gctgatgcag ggcgacaatc acgcccggct cgctcatggc
    16501 ctttaacagg cgacgaaaac tgtgctgggc atcctgcacg ggaagcataa aagcggtttc
    16561 cagggtcatg cgttgtctcc gcgaaccatc gtaaagaagt cgacccggct ggcgttcact
    16621 tcggcctggc gcgcggcaat gcgtgccata cggtcagcgt ccagcggggc gataagggtt
    16681 tctgataagt tttgaaagtg gcggttttgc tgcatcagcg catcaatcag cgcacaacgt
    16741 tcggcgtgct gtttgtcgcg ccccagcacc cagctatagc cgagcgtgcc gtcagtcaga
    16801 cgcacggcgg cgcgggtgag cgtggcgtcg ccagcaaaaa agcgttcgcc ggtgccgccc
    16861 attcgcgcct gaatctgcac caggccagtt tcagcggcgc ggatcacctc ataatcggcg
    16921 ttgatgttca gcgcgttcag gcgggctgcc agttcagccg gttggctgtg cgccagcacg
    16981 gacatccagt gctggcgggt cgcggtatct gcgtgcattc agtgctccat agtgaattca
    17041 atcatgtcgg cgcgcgtcag gctgacggag tactccgccg ggctgctttc accgtcacgg
    17101 tggttaaggg tgcgcacgca cagcagcggc gacagattcg ggatttcaag acgctggcac
    17161 tctttggcct gggcgcgacg ggcgctgatc cgcgtctgac tgcgtcgtag cgtaatcccc
    17221 gtttgctcgc gcagaaaatc gtgcagcgaa ccgctgtcga aacgttgcag cgtcggccag
    17281 agggtgaggt ccgcaaagta gtggtcgatt aaacagagcg cgacgccgtt gacccgacgc
    17341 agggtgcgca ggtggatgac gttctccccc tcggttatcc ccagcgcgtc agcgacgtga
    17401 ccggaggcgg ggcgcagcac cgatagcaat ttttcgctgg tgggatggct gccctggtcc
    17461 agcaggttct ggctaaaacg cgcctgggcg ttgagcgggt aatcgaacgg gcgcatcagc
    17521 accagtacgc cgacgccctg acggcgctgt acccagcctt tttccaccag ttggtcgatg
    17581 gcgcggcgca gggtgtggcg gttcacctca aagcgcgccg ccagttgctg ctcggcggga
    17641 agatagtcgc cgcagcggta gtgttgacga agctcctgct caagttttgc ggctatctcc
    17701 tgatagcgtg ttgggtagct ggtcggatgt gtagacaagt gcatagatat caatgcctcg
    17761 cttatcagat aaagtgctta cgcaaccgtt gagagaggaa atccagcagg ctgaccgtga
    17821 cgatgatgag caccatcagg gcgcaggttt gctggaactg gaaaccgcga atcgcttccc
    17881 acagcgtgac gccgatcccg cccgcgccaa ccataccgac gacggtcgcc gagcggacgt
    17941 tggattcgaa gcgatagaga gagtaggaaa tcagcagcgg catgacctgc ggtagtacgc
    18001 cgtaaaggat ctcttccagc ttgttggcac cggtggcgcg aatgccctcc accgggccgg
    18061 gttcgatcgc ttccaccgct tcggaaagca gcttggagag cacgccggtg gtgtggataa
    18121 acagcgccag cacgccagcg aacgggccga ggccgacggc gaccacgaac agcatggcga
    18181 agaccatttc gttaatggcg cggcaggcgt ccatcaggcg gcgtacaggt tggtagaccc
    18241 accacggtac caggttttca gcgctcatca ggccgaaggg gatggagaga accaccgcca
    18301 gcgcggtgcc ccagacggcg atttgcagcg tgacggccat ttcggtgagg taatcctgcc
    18361 actggctgaa atcgggcggg aagaagtcgg cggcgaacgt cgccatgttg ccgccatctt
    18421 taatcagcgt aagcggggcc atttccgcgc cctgccacga gacgaccagc acggcgataa
    18481 caacggccca gctcagaagc gagaaccagc tgcgcttggg tggggcgatg gtgatggttt
    18541 gcatgtttgg ctccggtctg taggccggat aaggtgcttg caccgcatcc ggcatcaacg
    18601 tctgcacatt gtcggatgcg acgccgggcg tcttatccga cctacgattt actgcaccgc
    18661 tttactcacc gaactcattg cgcttaacgc gttgttcagg cggtccaaat catccagttg
    18721 cgcctgaatg gcggtggttt ttgccagctt gtcctgttcg ttcagccctt tgttgtcctt
    18781 cacgctttgc atctctttaa acagtgcgag ctggcgaatc ggcaccagtt gcaagtcgct
    18841 ggaggcgcgg aacggcgccc agcccaggcg ctccagcacc gctttctctt ccggcgtttt
    18901 gccgtaattc ataaagaagt cgtagatctt gtctttagtg gtttcggaaa gatttttgcg
    18961 ccagacgatc ggatcgccag ggatcagcgg tgatttccag atcaccttca gttctttcag
    19021 cttctccggc gcggaggttt tcagcttgtc gaggttttcg gtgttgttgg tggcgacatc
    19081 cacttgcttg ttagcgacgg cgagggcgtt ggtttcatgc ccggcgttga cggtgcgctt
    19141 aaagtcgctg gcggagatat tgtttttagc gaagacgtag taaccgggga cgaggaagcc
    19201 ggaggtagag ttaggatcgc cattgccgaa ggtgagatct ttccgcttcg ccagcagatc
    19261 gttcaggttg ttgatcgggc tatctttgtt gacgatcaac acgctccagt aacccggcga
    19321 tccatccgcc gcgaccgtct gggcgaagac ctgaccgttg gcgcgatcca ccgcttccat
    19381 cgccgacagg ttgccgtacc aggcgatatc cactttattg aagcgcatcc cctggataat
    19441 gcccgcgtag tccggggcaa agaaggcgtt gacctttacg cccagcttct tctccatatc
    19501 ctgtaagaac ggcgtccatt gcggtttcag gttttgctgt gattccgttg aaataatgcc
    19561 gaaattcagc gccttttcct gctcttcggc gtatgccgga tttaacaggg tgctgaggct
    19621 gaacatgctg gtgaaggcca gcgaggcaat tatcttagcg ttcattcgtt ttcctcaatg
    19681 atggggatgt caggcagctt tcgcgttctc ttcgacgcgg ttaatgctgc ggtagagatg
    19741 gtcaaaacgt tcgttatcaa actgttggct gcagccgtcg tagaagacgt gcccctggcg
    19801 cagggcgacg atgcgttcgc agtagcgcag ggcgtaatcc acctgatgca gcgtgacgac
    19861 cacggtaatg ccgtcgttct ggttgatgtc gcgcagggtg tccatcacga tgcgtgccga
    19921 ttccgggtcc agcgaggcga tgggttcgtc agcgagaatg accttcgcct gctgcatcag
    19981 cgcgcgggca atcgccacac gctgttgctg gccgccggag agtgtggaaa cgcgttgatg
    20041 ggcaaagtgc accatgccaa cgcgggtcag cgcctgtaac gcgcgctgtt tctgctcgcg
    20101 ggtgaagtag ctaaaacagg ttcgccagaa tggcgtgctg ccgagcgcgc caatcagcac
    20161 gttctccagt acgctcaggc gattcaccag gttgaattgt tgaaagatgt agccggtgtt
    20221 ggcgcggctt ttgcggatat cgcgggccag gcggccttcg cgctggactg tgcggcccag
    20281 cagctcgata tggctaccga cagatttatc gccggtaatc aaaccgctta agtgacgtaa
    20341 aagggtggat tttccggaac ccgacggccc aagcagagcc accatttcac catgatgaat
    20401 gttcagatca accgcgtgca gcgcctgatg ctgattgaag gttttggcga gcttctcgac
    20461 acggataatc gtttgcatga tgcagcctcc ctaaaaaagt ggctccatcg tggcggatta
    20521 ttgtgacgat ttggttaatt aaaagtgact aacagatgaa gagttaacgg gaattcgatg
    20581 acagtgcggg agggcgggcc tcccggcggg ttattgcgtt ggctgttgtt tgatgacatt
    20641 aatcatccac ggtacgccaa atttatcggt gactttgcca aagccatgcg cccagaacgt
    20701 ttcctgccag gccatttcga tttttccgtt ggcggcaagg ttatcaaacc agcgttttcc
    20761 ttcttccacc tgttgagaat cgagcaccag cgtaaagccg gagtagctgg cttttcctga
    20821 cggtatagca tcgctcatca tgatgtcgct tcctgcaatg cgcacattgg cgtgggcgat
    20881 ggcggtatcg ggaaattgca ttccggaagg gcagttctcg gcgctgtcct gcgttgattt
    20941 tggcatttcg ccgaagctga ttttatagag cagttccgcg cccaacgtac gttgataata
    21001 ggcaatcgcg tcggaacagt taccggcaaa agagaggtag ggacttaacg gcatgatggt
    21061 gacctcagtt aagagaagcc agttaagtgt agttcgaaat ttatacagat gagagggaag
    21121 cctgataaga cgcggtcagc gtcgcatcag gcagtcgcca ccgttgccgg atgcggcgcg
    21181 agcgccttat ccggcctacc gatcacaata caatcagttc tttttcacaa actcagactt
    21241 cagtttcatc ggaccaaaac cgtcgatttt gcaatcgatg ttatggtcgc cttcaaccag
    21301 gcggatgttt tttactttgg tgccaatttt cagcatcgaa gagctacctt tcactttcag
    21361 atctttaatg atggtaacgc tgtcgccgtc agccagcaga ttgccgttag catctttaac
    21421 gatcagctcg tcgctttcct gtgcaggttc tgcgtcgttc cattcgtagg cacattccgg
    21481 gcagatgtac atgccgttat cttcgtaggt gtattcggag ttgcattttg ggcagtgtgg
    21541 taatgacatg tggatttcct caaaagtcag cagggcaaaa gcgccaaaaa acggcagatt
    21601 gccgaaaaag gccgcaatta tacacaaaat ccctgagttt gtcgggggta ctgctgattt
    21661 tagatgacga tcgttttcat ttgaaaggaa acgcaaaaag aaaataatct ttttgcctcc
    21721 atttattatt taagataagg ttaatgctct actcgttctt tttttaattc acatgatgtt
    21781 tttatctttc tgacgtttta aacgtattgg gagatgttat ttattctaat aaaaaaacaa
    21841 cacgctaaat ataaaattat ttcctgcagt aattataaaa gccgaaaaca gaactcaaaa
    21901 acaatctggc taaataaaag taataaaatt tgctttaagg aagaatattc tatgtacaca
    21961 cagaccctgt atgagttaag tcaggaggct gaacgcctgt tacagctttc ccgacaacag
    22021 ttgcagttac tggaaaaaat acctctctct gtacccggag acgatgcgcc acaactggct
    22081 ttaccctgga gtcagcctaa tatcgccgaa cgtcacgcga tgctgaataa tgagttgcgt
    22141 aaaatttccc ggctggaaat ggtgctggcg attgtcggta ccatgaaagc agggaaatca
    22201 accaccatta atgccattgt tggtacggag gttctgccta atcgcaatcg cccaatgact
    22261 gcgctgccaa cgcttattcg ccatacgccc gggcaaaaag aaccggtact gcatttttca
    22321 catgtcgcgc caatcgacca tttaattcaa caattacaac agcgcctgcg tgattgcgat
    22381 attaagcatc tgaccgatgt gctggaaata gataaagata tgcgtgcgct tatgcagcgg
    22441 atcgaaaatg gcatcgcttt cgaaaaatat tatctgggtg ctcaacctat ttttcattgt
    22501 ctgaaaagtt tgaatgattt ggtgcgactg gcgaaggcgc tggacgtcga ttttcctttt
    22561 tctgcttacg ccgccattga gcatattccc gtgattgaag tggagtttgt ccatctggcg
    22621 gggctggaga gttatcccgg tcagttgacg ttactggata cccccgggcc aaatgaagcc
    22681 gggcaaccgc atctgcaaaa aatgcttaac cagcagctgg cacgcgcctc ggcggtactg
    22741 gcggtgctgg attatacgca actgaaatcg atctccgatg aagaggtccg tgaggcaatt
    22801 ttggcggtgg ggcaatcggt gccgttgtat gtgctggtca ataagttcga tcaacaggat
    22861 cgtaacagtg acgacgccga ccaggtgcgg gcactgattt ccggaacgct gatgaaaggc
    22921 tgtattacgc cacagcagat atttccggtg tcgtcgatgt ggggctacct ggcgaatcgg
    22981 gcgcgccatg agttagccaa caacggtaag ttacccgcgc cagagcaaca acgctgggtg
    23041 gaagattttg cccatgccgc gctcggcagg cgctggcgtc atgccgatct ggcggacctc
    23101 gaacatattc gccatgctgc cgatcagttg tgggaagatt cgctgttcgc ccagccaatt
    23161 caggcgttgc ttcatgccgc ttacgctaac gcctcgttgt atgctctgcg atctgccgcg
    23221 cataaactgt tgaattacgc gcagcaggcg cgggaatacc tggattttcg tgcgcacggg
    23281 ttaaacgtcg cttgtgaaca attgcggcaa aatatccatc aggtcgaaga aagtttgcag
    23341 ctattgcaac tcaatcaggc gcaggtgagc ggcgagatta aacatgaaat cgagttggcc
    23401 ctgacctccg ccaaccactt tctgcgtcaa cagcaagatg cggtgaatgc gcagttagcc
    23461 gcgttgtttc aggataattc ggagtcgtta agtgagatgc gtacccgctg tgagacactg
    23521 ttacagacgg cgcagaacac catcagtcgc gactttacgc tgcgctttgc cgagcttgag
    23581 tccacccttt gccgggtgtt aaccgatgtt attcgcccca ttgagcaaca agtcaaaatg
    23641 gaattgagtg agtctgggtt tcgtcctggg tttcattttc ctgtttttca cggcgtagtt
    23701 ccccacttca acacccgcca gctgttcagt gaagtcattt cgcgccagga agcaacggac
    23761 gagcagagca cgcgtttagg cgttgtgcgt gagatttttt cgcgctggtt gaatcagccc
    23821 gactggggac ggggaaatga gaaattcccg acagagacgg ttgattacag tgtgttgcaa
    23881 cgagcattaa gcgcagaagt cgatctttat tgccaacaaa tggctaaagt tatggcagag
    23941 caggtcgatg aatctgttac ggcaggcatg aatacttttt tcgctgagtt cgcttcatgt
    24001 ttgacggaat tacagacgcg tttacgcgaa agtatggctc tgcgtcaaca aaatgaatcg
    24061 gtggtcaggc tgatgcaaca gcaattgcag cagactgtga tgactcacgg ctggatttac
    24121 accgacgctc agctgttacg cgatgatatt caaacacttt tcacggcaga acgatattga
    24181 ccaagacgtt actggacggc cccggtcgcg tgctggagtc ggttcatccg cgctttttag
    24241 tggatctggc gcagggcgat gatgcccgcc ttccacaagc ccatcagcag cagtttcgtg
    24301 aacgactgat gcaggaactt cttgcgcgcg tgcagcttca gacatggacg aacggcggca
    24361 tgttaaatgc gccgcttagc ctgcgtctga cattggtgga aaaactggcg tcgatgctgg
    24421 atcccggtca tctggcgctg acgcagatcg cgcagcatct ggcgctgctg caaaaaatgg
    24481 atcaccgcca gcactctgct ttcccggagc tcccccagca aattgtcgcc ttgtatgagt
    24541 ggttttcagc ccgttgtcgc tggaaggaaa aggcgttaac gcaacgaggc ctattggtgc
    24601 aggcgggtga acagagcgag caaattttta cccgctggcg tgctggggcg tataacgcct
    24661 ggtcgttgcc tgggcgctgt tttatcgttc tcgaagagtt gcgctggggt gcatttggcg
    24721 atgcctgccg tctgggaagc ccgcaagcgg tggcgttgtt gctgggtgat ttgcgcgtga
    24781 aagcgacaca acatttggca gagagtatca atgcggcccc gaccacgcgt cactattacc
    24841 atcagtggtt tgcctcttcg accgtttcga cgggcgggga tcatgctgat tttttaagtt
    24901 ggctgggaaa gtggtctacg gcagataaac aacccgtttg ctggtcagtg acccaacgct
    24961 ggcgaactgt cgcactgggg atgccacgac tctgttcagc gcagcgtctg gcgggggcaa
    25021 tggtcgagga aatcttctct gtaaatttgg tgtaaataat cagttacacc aatgagtcct
    25081 aaacgaaatg catgtgtgaa gttgatcaca aatttaaaca ctggtagggt aaaaaggtca
    25141 ttaactgccc aattcaggcg tcaactggtt tgattgtaca ttccttaacc ggagggtgta
    25201 agcaaacccg ctacgcttgt tacagagatt gcatcctgca attcccgctc cccttttgcg
    25261 gccgtcgcgc tgatttttct ggcgtttgcg gaaatgggcc aactctgcga ggaaagctat
    25321 gctgaaaagg aaaaaagtaa aaccgattac ccttcgtgat gtcaccatta ttgatgatgg
    25381 taaactgcgt aaagccatta ccgcagcatc actgggtaat gcaatggaat ggttcgattt
    25441 tggtgtttat ggttttgttg cttacgcatt aggtaaagtt tttttcccgg gggctgaccc
    25501 cagcgtgcag atggttgctg cactcgccac tttctccgtt ccctttctga ttcgaccgct
    25561 tggcgggctc ttctttggta tgttgggcga taaatatggt cgccagaaga tcctcgctat
    25621 cactattgtg attatgtcga tcagtacgtt ctgtattggc ttaataccgt cctacgacac
    25681 gattggtatt tgggcaccga ttctgctgtt gatctgtaag atggcacaag gtttctcggt
    25741 cggcggtgaa tataccgggg cgtcaatatt tgttgcggaa tactcccctg accgtaaacg
    25801 tggctttatg ggcagctggc tggactttgg ttctattgcc gggtttgtgc tgggagctgg
    25861 cgtagtggtg ttaatttcga ccattgtcgg cgaagagaac ttcctcgatt ggggctggcg
    25921 tattccgttc tttatcgctc tgccgttagg gattatcggg ctttacctgc gccatgcgct
    25981 ggaagagact ccggcgttcc agcagcatgt cgataaactg gaacagggcg accgcgaagg
    26041 tttgcaggat ggcccgaaag tctcgtttaa agagattgcc accaaacact ggcgcagcct
    26101 gttgacatgt attggtctgg taattgccac caacgtgact tactacatgt tgctgaccta
    26161 tatgccgagt tatttgtcgc ataacctgca ttactccgaa gaccacgggg tgctgattat
    26221 tatcgccatt atgatcggta tgctgtttgt ccagccggtg atgggcttgc tgagtgaccg
    26281 ttttggccgt cgtccgtttg tgctacttgg tagtgttgca ctgtttgtgt tggcgatccc
    26341 ggcgtttatt ctgattaaca gtaacgtcat cggcctgatt tttgccgggt tactgatgct
    26401 ggcggtgatc cttaactgct ttacgggcgt tatggcttct accttgccag cgatgttccc
    26461 gacgcatatc cgatacagcg cgctggcggc ggcatttaat atttcggtgc tggttgccgg
    26521 tctgacgcca acactggcgg cctggctggt cgaaagctcg cagaatctga tgatgcctgc
    26581 ctattacctg atggtagtgg cggtggttgg tttaatcacc ggcgtaacca tgaaagagac
    26641 ggcaaatcgt ccgttgaaag gtgcgacacc ggcggcgtca gatatacagg aagcgaagga
    26701 aattctcgtc gagcattacg ataatatcga gcagaaaatc gatgatattg accacgagat
    26761 tgccgatttg caggcgaaac gtacccgcct ggtgcagcaa catccgcgaa ttgatgaata
    26821 agctgaaacg gatggcctga tgtgatgctg tcttatcagg tcaattgaac tcttaaggtt
    26881 cacttaatct ctgacgcgca tactctcctc caggttaacg gaggagagtg caatgaaaaa
    26941 ccgtgtttat gaaagtttaa ctaccgtgtt cagcgtgctg gtggtcagca gctttcttta
    27001 tatctggttt gccacgtact gatctttttt cagccgtatc caggcccgcg tgccggaagt
    27061 ctcttgtcgg ttttgcagga aaaactgccc gtgatgcaac tgtgtaatgc ggctgacaat
    27121 acttaacccc agaccaattc cgccataacg gctgtccatt cgtacaaatg ctttactcaa
    27181 ctccccgcat ttactctcat caatacccgg tccttcatct tcaacggcca tgaccgctcc
    27241 gccatcttct tgcagcttaa tcataatgtt gctgccttgc gggctataac gatgggcgtt
    27301 ttctaccagg tttcgcaata acatccgcag cagggttgca tcaccctgaa cggtgatgtc
    27361 ggcggcgctc tctggcaata gcagggtttg ctggcgctgg tcgagcatgg tactgagttc
    27421 gtcatacgag gggagaatga catcttccag cagttttaca tgttgataat taccggaaga
    27481 aaatgactgt ccggcacgcg ccagttgcag caactgggag acgctctcca tcatctgatc
    27541 aagtcgtgcc actaacggtg ctacatcaat gtgatgcgtt ttcgccagca gttccagatg
    27601 caaacgcacc cccgccagtg gcgttcgcag ttcgtgcgcg acgtcagcgg taaacaacct
    27661 ttcattatcg agcgtgctgg tcaggcgact gaccagatcg tttaacgccg aaaccaccgc
    27721 atcgatttcg agggtggcgc tatgaatggc aatgggcgtt aagttgtcgg cggtgcgcgc
    27781 ttccagctct ttttgcagct ccgccagcgg tcgggtgatg cggcgtaccg cctgatagca
    27841 gataaatagc gtcaggctaa ccataaagac gccggggaca atcaggctgg cgaccgcctc
    27901 gcggatctca cgcatgatgt ggcgatcgtt gttgcgattg tcgcgtagcg cctgctcaaa
    27961 cagctgaatc tgctcggtac tttcatgcca tagccagaag acgctgatca gctcaaacac
    28021 caacaaaatg gccccgatgg tcaatatcag ccgttggcgc agcgatattg gtcggcgcag
    28081 aaaacgcatc agattcaatt agttttcctc atttgcgacc agcatatagc caaatccgcg
    28141 cacggtgcgg atacgggctt tgcccacttt gtcgcgcagg ttatggatat gcacttccag
    28201 ggtgttggtc gagggctcgt tgtcccagtt gtagatatcg ttgtagagaa tttcccgatg
    28261 caccgggctg cctgctttaa gcatcaggcg cgacagcaga gcatattctt tgggcgtcag
    28321 aatcaactct tcaccgccca tccagacctg acggcgaccc atattcagcg tcagattgcc
    28381 aacaatcagc tcactttcgc cctgattatt atggcgtcgt agcagggcgc ggatacgggc
    28441 gtgcaactct tccagcgcaa aaggcttcac cagataatcg tcggccccga catccagccc
    28501 ggcgattttg tcggtaaggg tatcgcgggc ggtgagaatt aacaccggca gagtgtattt
    28561 tttctgccgg atacgggcaa gaaaatgcag cccatcttca tcgggtaagc ctaaatccag
    28621 taccaccagg ctgtaatgac cggcttcaag gctttgttcc gccatccgcg cggtgctcac
    28681 gccatcgcac gcgtagcctt cggtttgcgc cgccagaatc aatccctgca ataacagcgt
    28741 atcgtcttca acaatcagaa ttttcattca ctcactctcc tgcaagtttg cagaatatca
    28801 tccgcagcct ggtaatactt cgtctcaacg ccagtcaggc ccaataaggt tgagaataaa
    28861 ttgtcttgtg aatagtgttg cgtttgcgcc tgtttttgca ggcagttctg gtcaacctga
    28921 taccgttttt gataatcctc cgatagccac agcagcatcg gcacctgttt ttggctatcc
    28981 ggggcgatgg cataaggcag accgtgcaga tagatgccat tttcacctaa cgattcaccg
    29041 tggtcagaaa gataaaccag gctggtggta aatttatcct gatgttcttt cagcagatta
    29101 atcgctttat caacaatata gtcgacgtaa accagcgtgt tgtcgtaagt gttcaccagt
    29161 tgctcttggg tacaggtctg gatctcgtta gtgtcgcagg ttggggtaaa tttcctgaac
    29221 tgcggcggat agcggttgta ataggtcggg ccgtgactgc cgatggtgtg taagacaatc
    29281 acgccatcac cctgcaggtt attgatgtaa tcttccagcc cgtggaacag cacttcatca
    29341 tagcattcgc cgttgatgca ctgaccaggc aggttcagcg cggtgatgtt ctggtgaggt
    29401 acgcgatcgc aaacgccttt acagccgcca tcgttgtcat tccacagcac gttgatgccc
    29461 gctcgctgaa tgatatccag cacgccttcc tggtgctgtg ccagctcttc tttgtagtgc
    29521 tcacgcggca tatccgagaa catgcatggt actgaaactg ccgttgccgt gccgcaagag
    29581 gcggtattag ggaaatagac cacgttatct tttgccagac gaggattggt ttcgcgtgga
    29641 taaccattca gcgagaaatt ctctgcgcgc gacgtttctc cgacgatcaa aatggtcagg
    29701 tttttacgtt tcccattttg cattaatggg ttgcggtgag catcttcacc aatgcgtatc
    29761 agtggcaggt tggctgaccg ctggtgagag taccacgacc agctagcaac aatgctgttg
    29821 gacgggctta aggatttcac cagttctttg ttgttgcgaa acagcgaggc atagtctttg
    29881 tagaacagtg cggcaaccag cagaatcagc agtacagaaa tcaggatgtt ggccccgcgg
    29941 aaaagaacgc tgcgcaggcg tgaggtggtg ggtttgattt tgatccagca ggcaataatc
    30001 gcggctaata cgccacttaa ccccagcgtt aataacattc gtggcgtcat cagggcataa
    30061 ctttccgccg gcgtagtatc aatgatattg gtgatcattg aacggtcgat gacgatgcca
    30121 taggtcatga tgaaatattg cgcagccgca ccgaccagaa taaacaggca ggcgagtgga
    30181 cgattaagcc ataagaaaga gcctagtgtc aggacaatat taatcacgct gaatgcgacg
    30241 accggcatcg acaagaaaac cagcacgtta tgcagtgaat ccagcggtaa cacctgtagc
    30301 acctgtttaa aaaaggcgat attcaggcag actgagatat aaaaagtagc aagtaataac
    30361 caggagaata aattcaaaga gggtcttttt agtaggcgct tcaacatcac ggtgtttcca
    30421 tcgaacaaag tgcgcatatg ctcgcaaagc aaaattaagc caaccttaag ttcttaaggt
    30481 tggcttttat gtttgctgga ttaaggggaa ttaatctttg cttattggtg catctaaggg
    30541 atacgggttt ttatgcagcc ggttgtaatt cagcgcatac atggcggtga ttaccatcag
    30601 agtgacaaat gaccacataa cctctttcgc gccggaccct actacggccc agatgcagta
    30661 gaggaaggca atggtagtaa ctgccaggta tgccgggcgc gctttaccaa agtgaccgtg
    30721 gccgagcagc agtaacgccg cacaggtgta aagatatggc accagtgtaa agatgaccga
    30781 cacggaagaa accagaccga attctttggt cgcgtttggc gaaatgctac tgagctggaa
    30841 gatggtcatc aaaataccga cgataatcag ccccgccact ggcgtgcccg ctttatttac
    30901 acgggcaaaa atcggtggga acagtccgtc atcggcagcg gctttcgccg tttgacctgc
    30961 cagcaacgtc cagccgccca gtgaacctaa gcaacccgca gctgcgcaga aggaaacaat
    31021 ggccccggcg gtgtcaccca acgccatccg tgcggcatca ccgaatggcg aagcagaaac
    31081 gcgcagtgcg gcattaggga tcatccccat aatcgcggtg gtagaaagta catagcaaac
    31141 ggcagcaatc aataccccgc caatggtggc gatagggacg ttgcgtttcg ggtttttcac
    31201 cacacctgcg gcaacggagg cactttccac accgatgaac gaccacagcg taacgttaag
    31261 ggtactttga atcgcaccga aggtgcccag gccgctgacg ttccatgccg ccatataggt
    31321 ttcaccacgg aaccagaacc agccaaatac ggcaatcccg acgatgggga tcagcgccag
    31381 cacggtagca acggcctgta cgcgggtgat cattttcgga ccgacaatgt tcagcaggac
    31441 gaagatccac agcaccacga cgcaggtgat ggttaatacc aatggatctt tcagaatcgg
    31501 gaagaagtaa cttaaatatc ctacgccaat gaccaccatg gcgatattgc cgatccagca
    31561 ggccagccag tagaggacgt tggtttgata accgagaaac ggcccaaagc agcggcgggc
    31621 gtaagcgtaa gaaccaccag gacttgggtc gaggaacgac attttggcgt ataccatcga
    31681 gagccccagc gcaccgataa tcgtcaccaa ccatccataa atggcaatcc cgccagtgga
    31741 ggccaggttt gcaggtaaca gaaaaacacc tgaccccata atattccccg acaccatcag
    31801 ggtgacgggg attaagccca ctttgtgagc atcagcatcc gaagacataa ttaaactcct
    31861 gcgaaggcga gcttcgtgac aataaattac gtcatatcat acgcctgcat tcgatagttg
    31921 aattattatc gaccgggtta tcgctgatgc cgttattaat atagctcttc gttaaggtta
    31981 aaaatcaggc gacagtgcgt tctctgtgct gactaacata atgcagaggc gtcataccgt
    32041 agaagtcttt aaagacagaa ataaagtacg acgtactgtt gtagccgcag gactgtgata
    32101 cctgagagat atttttaccg tccatcatta attcatttac ggcatagcgc atgcggcagg
    32161 tggtgattat ttggctataa ctggtgtttt cgcttttcag ctttttcttt aacagacttg
    32221 ggctaagaca taaacaactg gctaccatac taagattcca gtctttgtgg atatcgcttt
    32281 caataatttg ataaacgctg tcacttacac aattacgtaa catatacatc attagtgaaa
    32341 ggaatttttt actgtcgaga aaacgggata gcacagtgaa taataatgca cgtgttcgtt
    32401 ccttttctga ctcagtttca gcgggcatca tgctatgctg cgcggcaacg cgaaagacgt
    32461 ctggcgtcag gcatggaagg gtgaggatgg gcgtagcgct acgttgccat actggtattt
    32521 gcgagagatc tttattcaga aaccggaggt aatctttgat gatgtcgtga ctaatatgag
    32581 caaccaaagt gttattaaga gaggagacgt cgataatatt attttcacaa tttaataacg
    32641 ccatgtggtt agctttaagg ctaataggtt ctttcccatt cacccttatc cagacatctt
    32701 tttcagtcaa taaaacaata caaggttggt cgctgcaaat cctcatacat gtactcctga
    32761 gtgcgaataa aagaccagtt ctcgctaaag cgaagcgata ccgggaaatg gttatataat
    32821 tgtatgatga atataaacat gcataaaaat a
//
LOCUS       DAHHTG010000044        32276 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.044, whole genome shotgun sequence.
ACCESSION   DAHHTG010000044 DAHHTG010000000
VERSION     DAHHTG010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 32276)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32276)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32276
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.044"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            74..301
                     /gene="traY"
                     /locus_tag="NBI74_002038"
     CDS             74..301
                     /gene="traY"
                     /locus_tag="NBI74_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001254388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer relaxosome protein TraY"
                     /protein_id="HCD1932381.1"
                     /translation="MRRRNARGGISRTVSVYLDEDTNNRLIRAKDRSGRSKTIEVQIR
                     LRDHLKRFPDFYNEEIFREVIEENESTFKEL"
     gene            335..697
                     /gene="traA"
                     /locus_tag="NBI74_002039"
     CDS             335..697
                     /gene="traA"
                     /locus_tag="NBI74_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001668211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system pilin TraA"
                     /protein_id="HCD1932382.1"
                     /translation="MNTVLSVQGASAPVKKKSFFSKFTRLNMLRLARAVIPAAVLMMF
                     FPQLAMAAQGQDLMASGNTTVKATFGKDSSVVKWVVLAEVLVGAVMYMMTKNVKFLAG
                     FAIISVFIAVGMAVVGLK"
     gene            702..1013
                     /gene="traL"
                     /locus_tag="NBI74_002040"
     CDS             702..1013
                     /gene="traL"
                     /locus_tag="NBI74_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system protein
                     TraL"
                     /protein_id="HCD1932383.1"
                     /translation="MSGDENKLKKYRFPETLTNQSRWFGLPLDELIPAAICIGWGITT
                     SKYLFGIGAAVLVYFGIKKLKKGRGSSWLRDLIYWYMPTALLRGIFHNVPDSCFRQWI
                     K"
     gene            1035..1601
                     /gene="traE"
                     /locus_tag="NBI74_002041"
     CDS             1035..1601
                     /gene="traE"
                     /locus_tag="NBI74_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system protein
                     TraE"
                     /protein_id="HCD1932384.1"
                     /translation="MEHGARLSTSRVMAIAFIFMSVLIVLSLSVNVIQGVNNYRLQNE
                     QRTAVTPMGFNVPFAVSQNSADASYLQQMALSFIALRLNVSSETVDASHQALLQYIRP
                     GAQNQMKVILAEEAKRIKNDNVNSAFFQTSVRVWPQYGRVEIHGVLKTWIGDSKPFTD
                     IKHYILILKRENGVTWLDNFGETDDEKK"
     gene            1612..2316
                     /gene="traK"
                     /locus_tag="NBI74_002042"
     CDS             1612..2316
                     /gene="traK"
                     /locus_tag="NBI74_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003982266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system secretin
                     TraK"
                     /protein_id="HCD1932385.1"
                     /translation="MFGSLFFSCSVMAANGTLAPTVVPMVNGGQASIAISNTSPNLFT
                     VPGDRIIAVNSLDGALTNNEQTASGGVVVATVNKKPFTFILETERGLNLSIQAVPREG
                     AGRTIQLVSDLRGTGEEAGAWETSTPYESLLVTISQAVRGGKLPAGWYQVPVTKETLQ
                     APAGLSSVADAVWTGNHLKMVRFAVENKTLSALNIRESDFWQPGTRAVMFSQPASQLL
                     AGARMDVYVIRDGEGN"
     gene            2316..3743
                     /locus_tag="NBI74_002043"
     CDS             2316..3743
                     /locus_tag="NBI74_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001553749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraB"
                     /protein_id="HCD1932386.1"
                     /translation="MASINTIVKRKQYLWLGIVVVGAASAIGGALYLSDVDMSGNGEA
                     VAEQEPVPDMTGVVDTTFDDKVRQHATTEMQVTAAQMQKQYEEIRRELDVLNKQRGDD
                     QRRIEKLGQDNAALAEQVKALGANPVTATGEPVPQMPASPPGPEGEPQPGNTPVSFPP
                     QGSVAVPPPTAFYPGNGVTPPPQVTYQSVPVPNRIQRKVFTRNEGKQGPSLPYIPSGS
                     FAKAMLIEGADANASVTGNESTVPMQLRITGLVEMPNSKTYDATGCFVGLEAWGDVSS
                     ERAIVRTRNISCLKNGKTIDMPIKGHVSFRGKNGIKGEVVMRNGKILGWAWGAGFVDG
                     IGQGMERASQPAVGLGATAAYGAGDVLKMGIGGGASKAAQTLSDYYIKRAEQYHPVIP
                     IGAGNEVTVVFQDGFQLKTVEEMALERTQSRAEEENQESPVPVPPSAESHLNGFNTDQ
                     MLKQLGNLNPQQFMSGSQGGGNDGK"
     gene            3733..4317
                     /locus_tag="NBI74_002044"
     CDS             3733..4317
                     /locus_tag="NBI74_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001528624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraP"
                     /protein_id="HCD1932387.1"
                     /translation="MANNMSSRQACHAARYVVARVLRGLFWCLKYTVILPLATMALMA
                     LFVLWKDNTTPGQLLVQEIDFVRQTAPAGQFPVRECLPSLHDLPGAEEKICRYRAADA
                     ADYVRETDRSLMQLVTALWATLALMYVSLAVISGKYPVLPGKMKCIRGVTADERLTEV
                     YTEDASLPEKIRKCPVYFPDDRTNRNNGDKNEHA"
     gene            4271..4624
                     /locus_tag="NBI74_002045"
     CDS             4271..4624
                     /locus_tag="NBI74_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001377845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbD"
                     /protein_id="HCD1932388.1"
                     /translation="MTEQTETTEIKMNMRNINVITARSVPDKSVSDDFMHAVLSNCTT
                     RIVLPAPKEFSSESLPHNFNMAAVGVMKKGMSMSLFSSPGHIVVAGQSGGGKSVPVKE
                     LIDRIIQKYKEPFDE"
     gene            4617..4868
                     /gene="trbG"
                     /locus_tag="NBI74_002046"
     CDS             4617..4868
                     /gene="trbG"
                     /locus_tag="NBI74_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001038337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbG"
                     /protein_id="HCD1932389.1"
                     /translation="MNKLVSDGSVKKINYPVLYESGITPPLCEVSAPEPDAGGKRIVA
                     YVYKSSRNTVFENPDIVKTCTVRDLKNDFVNCDEKGEGQ"
     gene            4865..5380
                     /gene="traV"
                     /locus_tag="NBI74_002047"
     CDS             4865..5380
                     /gene="traV"
                     /locus_tag="NBI74_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system lipoprotein
                     TraV"
                     /protein_id="HCD1932390.1"
                     /translation="MKQISLFIPLLGTLLLSGCAGTSTEFECNATTSDTCMTMEQANE
                     KAKKLEQSSEAKPVAASLPRLAEGNFRTMPVQTITATTPSGSRPAVTALPEQKLLAPR
                     PLFTAAREVKTIVPVSSVAPVTPPRPLRTGEQTAALWIAPYIDNQDVYHQPSSVFFVI
                     KPSAWGKPRIN"
     gene            5515..5736
                     /gene="traR"
                     /locus_tag="NBI74_002048"
     CDS             5515..5736
                     /gene="traR"
                     /locus_tag="NBI74_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraR"
                     /protein_id="HCD1932391.1"
                     /translation="MSDEVDEAYSVTEQLTMTGINRIRQKINAHGIPVYLCEACGNPI
                     PEARRKIFPGVTLCVECQAYQERQRKHYA"
     gene            5896..8523
                     /gene="traC"
                     /locus_tag="NBI74_002049"
     CDS             5896..8523
                     /gene="traC"
                     /locus_tag="NBI74_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001064255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein TraC"
                     /protein_id="HCD1932392.1"
                     /translation="MNNPLEAVTQAVNSLVTALKLPDESAKANEVLGEMSFPQFSRLL
                     PYRDYNQESGLFMNDTTMGFMLEAIPINGANESIVEALDHMLRTKLPRGIPLCIHLMS
                     SQLVGDRIEYGLREFSWSGEQAERFNAITRAYYMKAAATQFPLPEGMNLPLTLRHYRV
                     FISYCSPSKKKSRADILEMENLVKIIRASFHGAKITTQTVDAQAFIEIVGEMINHNPD
                     SLYPKRRQLDPYSDLNYQCVEDSFDLKVRADYLTLGLRENGRNSTARILNFHLARNPE
                     IAFLWNMADNYSNLLNPEMSISCPFILTLTLVVEDQVKTHSEANLKYMDLEKKSKTSY
                     AKWFPSVEKEAKEWGELRQRLGSGQSSVVSYFLNITAFCKDNNETALEVEQDILNSFR
                     KNGFELISPRFNHMRNFLTCLPFMAGKGLFKQLKEAGVVQRAESFNVANLMPLVADNP
                     LTPAGLLAPTYRNQLAFIDIFFKGMNNTNYNMAVCGTSGAGKTGLIQPLIRSVLDSGG
                     FAVVFDMGDGYKSLCENMGGVYLDGETLRFNPFANITDIDQSAERVRDQLSVMASPNG
                     NLDEVHEGLLLQAVRASWLAKKKQARIDDVVDFLKNARDNDQYVESPTIRSRLDEMIV
                     LLDQYTANGTYGRYFNSDEPSLRDDARMVVLELGGLEDRPSLLVAVMFSLIIYIENRM
                     YRTPRTLKKLNVIDEGWRLLDFKNRKVGEFIQKGYRTCRRHTGAYITITQNIVDFDSD
                     KASSAARAAWGNSSYKIILKQSAKEFAKYNQLFPDQFQPLQRDMIGKFGAAKDQWFSS
                     FLLQVENHSSWHRLFVDPLSRAMYSSDGPDFEFVQQKRREGMSIHEAVWQLAWKKSGP
                     EMASLEAWLEEHEKYRSVA"
     gene            8520..8906
                     /gene="trbI"
                     /locus_tag="NBI74_002050"
     CDS             8520..8906
                     /gene="trbI"
                     /locus_tag="NBI74_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system protein TrbI"
                     /protein_id="HCD1932393.1"
                     /translation="MTTTQKTTDVTAPRRSHWWWTVPGCLAMVLLNAAISYGIVRLNA
                     PVTAAFNMKQTVDAFFDSASQKQLSEAQSKALSARFNTALEASLQAWQQKHHAVILVS
                     PAVVQGAPDITREIQQDIARRMRAEP"
     gene            8903..9535
                     /gene="traW"
                     /locus_tag="NBI74_002051"
     CDS             8903..9535
                     /gene="traW"
                     /locus_tag="NBI74_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system protein TraW"
                     /protein_id="HCD1932394.1"
                     /translation="MRCRGLIALLIWGQSVAAADLGTWGDLWPVKEPDMLTVIMQRLT
                     ALEQSGEMGRKMDAFKERVIRNSLRPPAVPGIGRTEKYGSRLFDPSVRLAADIRDNEG
                     RVFARQGEVMNPLQYVPFNQTLYFINGDDPAQVAWMKRQTPPTLESKIILVQGSIPEM
                     QKSLDSRVYFDQNGVLCQRLGIDQVPARVSAVPGDRFLKVEFIPAEEGRK"
     gene            9532..10524
                     /locus_tag="NBI74_002052"
     CDS             9532..10524
                     /locus_tag="NBI74_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus assembly protein TraU"
                     /protein_id="HCD1932395.1"
                     /translation="MKRSLWLLMLFLLAGHVPAASADSACEGRFVNPITDICWSCIFP
                     LSLGSIKVSQGKVPDTANPSMPIQICPAPPPLFRRIGLAIGYWEPMALTDVTRSPGCM
                     VNLGFSLPAFGKTAQGTAKKDEKQVNGAFYHVHWYKYPLTYWLNIITSLGCLEGGDLD
                     IAYLSEIDPTWTDSSLTTILNPEAVIFANPIAQGACAADAIASAFNMPLDVLFWCAGS
                     QGSMYPFNGWVSNESSPLQSSLLVSERMAFKLHRQGMIMETIGKNNAVCNEYPSPILP
                     KERWRYQMVNMYPDSGQCHPFGRSVMRWETGKNPPNTKKNFGYLMWRKRNCVFL"
     gene            10554..10859
                     /locus_tag="NBI74_002053"
     CDS             10554..10859
                     /locus_tag="NBI74_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001505125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1932396.1"
                     /translation="MATMDSKYIQHLRKIGAVLLRLISRTLKLFPFGLVIVPVILFYF
                     APDSFLAEIIHEWKKADISEQVTLVRLYLKVTFTCVFMAVFLNYLFSDEFMPVNSRK"
     gene            10868..11506
                     /gene="trbC"
                     /locus_tag="NBI74_002054"
     CDS             10868..11506
                     /gene="trbC"
                     /locus_tag="NBI74_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001733892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     assembly protein TrbC"
                     /protein_id="HCD1932397.1"
                     /translation="MNRKIFALFALTAGMHLAVHASENVNTTENRQFLKQQENLSRQL
                     REKPDHQLKAWAEKQVLENPLQRSDNHFLDELVRKQQASQDGKPRQGALYFVSFSIPE
                     EGLKRMLSETRHYGIPATLRGMVNNDLKATADAVLSLVKDGATDGVQIDPTLFSQYGI
                     RSVPALVVFCSQGHDIIRGNLRVRQALEKVAATGDCRQVAHDLLAGKGDSGK"
     gene            11503..13311
                     /gene="traN"
                     /locus_tag="NBI74_002055"
     CDS             11503..13311
                     /gene="traN"
                     /locus_tag="NBI74_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001756807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system mating-pair
                     stabilization protein TraN"
                     /protein_id="HCD1932398.1"
                     /translation="MKRILPLILALVAGMAQADSNSDYRAGSDFARQIQGQGTGSIQS
                     FKPQESIPGYNANPDETKYYGGVTAGGDGGLKNDGTTEWATGETGKTITESFMNKPKD
                     ILSPDAPFIQTGRDVVNRADSIVGNTGQQCSAQEISRSEYTNYTCERDLQVEQYCTRT
                     ARMELQGSTTWETRTLEYEMSQLPAREVNGQYVVSMTSPVTGEIVDAHYSWSRTYLQK
                     SVPMTITVLGTPLSWNAKYSADASFTPVQKTLTAGVAFTSSHPVRVGNTKFRRHTAMK
                     LRLVVRVKKASYTPYVVWSESCPFSKELGKLTKTECTEAGGNRTLVKDGQSYSMYQSC
                     WAYRDTYVTQSADKGTCQTYTDNPACTLVSHQCAFYSEEGACLHEYATYSCESKTSGK
                     VMLCGGDVFCLDGECDKAQSGKSNDFAEAVSQLAALAAAGKDVAALNGVDVRAFTGQA
                     KFCKKAAAGYSNCCKDSGWGQDIGLAKCSSDEKALAKAKSNKLTVSVGEFCSKKVLGV
                     CLEKKRSYCQFDSKLAQIVQQQGRNGQLRISFGRAKHPDCRGITVDELQKIQFDRLDF
                     TNFYEDLMNNQKIPDSGVLTQKVKEQIADQLKQAGQ"
     gene            13338..13595
                     /gene="trbE"
                     /locus_tag="NBI74_002056"
     CDS             13338..13595
                     /gene="trbE"
                     /locus_tag="NBI74_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000864342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbE"
                     /protein_id="HCD1932399.1"
                     /translation="MKVIFTSNRFIDFLIRLLITAIVISPVIIWSWDTVKETTADGML
                     AAAFVILYSGVLLFILYFCFSALTGLQKADERKNDERNEDE"
     gene            13588..14331
                     /gene="traF"
                     /locus_tag="NBI74_002057"
     CDS             13588..14331
                     /gene="traF"
                     /locus_tag="NBI74_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     assembly protein TraF"
                     /protein_id="HCD1932400.1"
                     /translation="MNKALLPLLLCCFIFPASGKDAGWQWYNEKINPKEKENKPVPAA
                     PRQEPDIMQKLAALQTATKRALYEAILYPGVDNFVKYFRLQNYWTQQAGLFTMSAKKA
                     MLAHPELDYNLQYSHYNGTVRNQLAADQAQQRQAIAKLAEHYGIMFFYRGQDPIDGQL
                     AQVINGFRDTYGLSVIPVSVDGVINPLLPDSRTDQGQAQRLGVKYFPAMMLVDPKQGS
                     VRPLSYGFISQDDLAKQFLNVSEDFKPNF"
     gene            14347..14685
                     /gene="trbA"
                     /locus_tag="NBI74_002058"
     CDS             14347..14685
                     /gene="trbA"
                     /locus_tag="NBI74_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001287890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbA"
                     /protein_id="HCD1932401.1"
                     /translation="MSEDYLKMFTGVVLLIFVIIAGYFFSERNDRKMFLLSSLVFLVI
                     NIACLYVLTASLWFLCGAIMNQGAAMVVPMVIGSLPNVTSFDRFRRIFICIMLSSAWS
                     GVMWFFIRGL"
     gene            14812..15096
                     /gene="traQ"
                     /locus_tag="NBI74_002059"
     CDS             14812..15096
                     /gene="traQ"
                     /locus_tag="NBI74_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001617873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     chaperone TraQ"
                     /protein_id="HCD1932402.1"
                     /translation="MISKCRFSLPRLDITGMWVFSLGVWFHIVARLVYSKPWMAFFLA
                     ELIAAILVLFGAYQVLDAWIARVSREEREALEARQQAMMEGQQEGGHVSH"
     gene            15083..15628
                     /gene="trbB"
                     /locus_tag="NBI74_002060"
     CDS             15083..15628
                     /gene="trbB"
                     /locus_tag="NBI74_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000052622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     assembly thiol-disulfide isomerase TrbB"
                     /protein_id="HCD1932403.1"
                     /translation="MSLTKLLPVILLMMATGVQASTRDEIERLWNPQGMAVPPAQPAA
                     DTSARTEKPAPRWFRLSNGRQVNLADWKVVLFMQGRCPYCHQFDPVLKQLAQQYGFSV
                     FPYTLDGQGDTAFPEALPVPPDVMQTFFPNIPVATPTTFLVNVNTLEALPLLQGATDA
                     ASFMARMDTVLQIYGEEKGAK"
     gene            15558..15899
                     /gene="trbJ"
                     /locus_tag="NBI74_002061"
     CDS             15558..15899
                     /gene="trbJ"
                     /locus_tag="NBI74_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001444236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-type conjugative transfer protein TrbJ"
                     /protein_id="HCD1932404.1"
                     /translation="MPPVLWRGWIPFCRYTERKKVRNKQVVLLIAGISGIATGIIVSL
                     NIPFIRQGLFYPASPVEIVVSLCLTFSVSVVFFVGAIVGWISVSYYSRITCLNESSEI
                     SEETYNERKKK"
     gene            15847..16272
                     /gene="trbF"
                     /locus_tag="NBI74_002062"
     CDS             15847..16272
                     /gene="trbF"
                     /locus_tag="NBI74_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001448114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F-type conjugal transfer protein TrbF"
                     /protein_id="HCD1932405.1"
                     /translation="MNHQKYQRKLIMKEKRNDAELKNRKTKRNYDYERRVSDIYFDLF
                     FVFVAAGTFLWVIMHSIFDACIDSWKADPALNNFRYMWNILMYVIPYTLWAFAGGFLI
                     VYVRNPLNELINGGIRIFRLKRRMRRENSFREGNNDASH"
     gene            16259..17632
                     /locus_tag="NBI74_002063"
     CDS             16259..17632
                     /locus_tag="NBI74_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001137361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraH"
                     /protein_id="HCD1932406.1"
                     /translation="MPRIKPLLVLCAVLLTVTPAASADVNSDMNQFFNKLGFASNVTQ
                     AGVWQGQAAGYAYGGSLYARTQVKNIQLISMTLPDINAGCGGIDAYLGSFSFINGDQL
                     QRFVKQIMSNAAGYFFDLALQTTVPEIKTAKDFLQKMASDINSMNLSSCQVAQGLVGG
                     LFPQTQESQQKVCQDIGSESNIFADWAASRQGCTVGGQSSSVRDKASDKDKERVLKNQ
                     NIIWEALKKNRMFDGNKELKEFIMTLTGTLIFGEDSEITPLPARTTDRDILRAIMEGG
                     TAKIYHCNDSDKCLKVVADANVTIARDKALKSQITKLLTSIQNKAVSDTPLDAREQGF
                     ISSTTIPVFKYLIDPQMLGVSNSVVYQLTDYIGYDIMLQYIQELLQQARAMIATGNYP
                     QAVMDNVLENLNQAQQQIAIFQSQVQVQQDALLVVERQMSYMRQQLSARMLSRYQNNY
                     HFGGNSL"
     gene            17629..20448
                     /gene="traG"
                     /locus_tag="NBI74_002064"
     CDS             17629..20448
                     /gene="traG"
                     /locus_tag="NBI74_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer mating pair stabilization
                     protein TraG"
                     /protein_id="HCD1932407.1"
                     /translation="MNEVYVIAGGEWLRNNLNAIAAFMNTRTWDSIEKIALTLSVVAV
                     AFMWVQRHNVMDLLGWVAVFVLISLLVNVRTSVQIIDNSDLVKVHRVDNVPVGLAMPL
                     SLTTRIGHAMVVSYEMIFTQPDSVTYSKTGMLFGANLIVKSTDFLSRNPEIINLFQDY
                     VQNCVLGDIYLNHKYTLEDLMASADPYTLIFSRPSPLRGVYDNNNNFITCKDASVTLK
                     DRLNLDTKTGGKTWHYYVQQIFGGRPDPDLLFRQLVSDSYSYFYGSSQSASQIMRQNV
                     TINALKEGITSNAARNGDTASLVNLATTSSMEKQRLAHVSIGHVTMRNLPMVQTILTG
                     IAIGIFPLLVLAAVFNKLTLSVLKGYVFALMWLQTWPLLYAILNSAMTFYAKMNGAPV
                     VLSELSQIQLKYSDLASTAGYLSAMIPPLSWMMVKGLGAGFSSVYSHFASSSISPTAS
                     AAGSVVDGNYSYGNMQTENVNGFSWSTNSTTSFGQMMYQTGSGATATQTRDGNMVMDA
                     SGAMSRLPVGINATRQIAAAQQEMAREASNRAESALHGFSSSIASAWNSLSQFGSNRG
                     SSDSVTGGADSTMSAQDSMMASRMRSAVESYAKAHNISNEQATQELASRSTRASAGMY
                     GDAHAEWGVKPKILGVGGGLGVRGGGRAGIDWSDDDAHQASSGSRASNDARHDIDARA
                     TQDFKEASDYFTSRKVSESGSHTDNNADSRVDQLSAALNSAKQSYDQYTTNMTRSHEY
                     AEMASRTESMSGQMSEDLSQQFAQYVMKHAPQDAEAILTNTSSPEIAERRRAMAWSFV
                     QEQVQPGVDNAWRESRGDIGKGMESVPSGGGSQDIIADHQEHQAIIEQRTQDSNIRND
                     VKHQVDNMVTEYKGNIGDTQNSIRGEENIVRGQYSELQNHHKTEALSQNNKYNEEKSV
                     QERMPGADSPQELMKRAKEYQDKYK"
     gene            20467..20964
                     /locus_tag="NBI74_002065"
     CDS             20467..20964
                     /locus_tag="NBI74_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000605858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="entry exclusion protein"
                     /protein_id="HCD1932408.1"
                     /translation="MIMRCLAHIALVTVIQFIACYLADWGSAETVFILCFIVLWQGLF
                     IWLFSQIRKKRHVSDEFKFSKGVWYITMPVSSLLSPLLSLMVFIIGTLYELRRVSGCI
                     SIKKWGQNQLKNQYDGSEKLDFGGIEQPPTTYYNPSTGYPMHGGFDSAGNTFGTRWQD
                     YYDRQ"
     gene            20996..21727
                     /locus_tag="NBI74_002066"
     CDS             20996..21727
                     /locus_tag="NBI74_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000850426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="complement resistance protein TraT"
                     /protein_id="HCD1932409.1"
                     /translation="MKTKKLMMVALVSSTLALSGCGAMSTAIKKRNLEVKTQMSETIW
                     LEPASERTVFLQIKNTSDKDMSGLQGKIADAVKAKGYQVVTSPDKAYYWIQANVLKAD
                     KMDLRESQGWLNRGYEGAAVGAALGAGITGYNSNSAGATLGVGLAAGLVGMAADAMVE
                     DVNYTMITDVQIAERTKATVTTDNVAALRQGTSGAKIQTSTETGNQHKYQTRVVSNAN
                     KVNLKFEEAKPVLEDQLAKSIANIL"
     gene            21980..24169
                     /gene="traD"
                     /locus_tag="NBI74_002067"
     CDS             21980..24169
                     /gene="traD"
                     /locus_tag="NBI74_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001589353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system coupling
                     protein TraD"
                     /protein_id="HCD1932410.1"
                     /translation="MSFNAKDMTQGGQIASMRIRMFSQIANIMLYCLFIFFWILVGLV
                     LWVKISWQTFVNGCIYWWCTTLEGMRDLIKSQPVYEIQYYGKTFRMNAAQVLHDKYMI
                     WCGEQLWSAFVLASVVALVICLITFFVVSWILGRQGKQQSENEVTGGRQLTDNPKDVA
                     RMLKKDGKDSDIRIGDLPIIRDSEIQNFCLHGTVSTGKSEVIRRLANYARKRGDMVVI
                     YDRSCEFVKSYYDPSIDKILNPLDARCAAWDLWKECLTQPDFDNVANTLIPMGTKEDP
                     FWQGSGRTIFAEAAYLMRNDPNRSYSKLVDTLLSIKIEKLRTFLRNSPAANLVEEKIE
                     KTAISIRAVLTNYVKAIRYLQGIEHNGEPFTIRDWMRGVREDQKNGWLFISSNADTHA
                     SLKPVVSMWLSIAIRGLLAMGENRNRRVWFFCDELPTLHKLPDLVEILPEARKFGGCY
                     VFGIQSYAQLEDIYGEKAAATLFDVLNTRAFFRSPSHQIAEFAAGEIGEKEHLKASLQ
                     YSYGADPVRDGISTGKEMERQTLVSYSDIQSLPDLTCYVTLPGPYPAVKLSLKYQARP
                     KVAPEFIPRDINPEMENRLSAVLAAREAEGRQMASLFEPDVPEVVSGEDVTQAEQPQQ
                     PQQPQQPQQPQQPVSPAINDKKSDSGVNIPAGGIEQELKMKPEEEMEQQLPPGISESG
                     EVVDMAAYEAWQQENHPDIQQQMQRREEVNINVHRERGEDVEPGDDF"
     gene            24169..29439
                     /gene="traI"
                     /locus_tag="NBI74_002068"
     CDS             24169..29439
                     /gene="traI"
                     /locus_tag="NBI74_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001463032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative transfer relaxase/helicase TraI"
                     /protein_id="HCD1932411.1"
                     /translation="MMSIAQVRSAGSAGNYYTDKDNYYVLGSMGERWAGKGAEQLGLQ
                     GSVDKDVFTRLLEGRLPDGADLSRMQDGSNKHRPGYDLTFSAPKSVSMMAMLGGDKRL
                     IDAHNQAVDFAVRQVEALASTRVMTDGQSETVLTGNLVMALFNHDTSRDQEPQLHTHA
                     VVANVTQHNGEWKTLSSDKVGKTGFIENVYANQIAFGRLYREKLKEQVEALGYETEVV
                     GKHGMWEMPGVPVEAFSGRSQAIREAVGEDASLKSRDVAALDTRKSKQHVDPEIRMAE
                     WMQTLKETGFDIRAYRDAADQRTEIRTQAPGPASQDGPDVQQAVTQAIAGLSERKVQF
                     TYTDVLARTVGILPPENGVIERARAGIDEAISREQLIPLDREKGLFTSGIHVLDELSV
                     RALSRDIMKQNRVTVHPEKSVPRTAGYSDAVSVLAQDRPSLAIVSGQGGAAGQRERVA
                     ELVMMAREQGREVQIIAADRRSQMNLKQDERLSGELITGRRQLLEGMAFTPGSTVIVD
                     QGEKLSLKETLTLLDGAARHNVQVLITDSGQRTGTGSALMAMKDAGVNTYRWQGGEQR
                     PATIISEPDRNVRYARLAGDFAASVKAGEESVAQVSGVREQAILTQAIRSELKTQGVL
                     GHPEVTMTALSPVWLDSRSRYLRDMYRPGMVMEQWNPETRSHDRYVIDRVTAQSHSLT
                     LRDAQGETQVVRISSLDSSWSLFRPEKMPVADGERLRVTGKIPGLRVSGGDRLQVASV
                     SEDAMTVVVPGRAEPASLPVSDSPFTALKLENGWVETPGHSVSDSATVFASVTQMAMD
                     NATLNGLARSGRDVRLYSSLDETRTAEKLARHPSFTVVSEQIKARAGETLLETAISLQ
                     KAGLHTPAQQAIHLALPVLESKNLAFSMVDLLTEAKSFAAEGTGFTELGGEINAQIKR
                     GDLLYVDVAKGYGTGLLVSRASYEAEKSILRHILEGKEAVTPLMERVPGELMETLTSG
                     QRAATRMILETSDRFTVVQGYAGVGKTTQFRAVMSAVNMLPASERPRVVGLGPTHRAV
                     GEMRSAGVDAQTLASFLHDTQLQQRSGETPDFSNTLFLLDESSMVGNTDMARAYALIA
                     AGGGRAVASGDTDQLQAIAPGQPFRLQQTRSAADVVIMKEIVRQTPELREAVYSLINR
                     DVERALSGLESVKPSQVPRQEGAWAPEHSVTEFSHSQEAKLAEAQQKAMLKGETFPDV
                     PMTLYEAIVRDYTGRTPEAREQTLIVTHLNEDRRVLNSMIHDAREKAGELGKEQVMVP
                     VLNTANIRDGELRRLSTWEKNPDALALVDNVYHRIAGISKDDGLITLQDAEGNTRLIS
                     PREAVAEGVTLYTPDKIRVGTGDRMRFTKSDRERGYVANSVWTVTAVSGDSVTLSDGQ
                     QTRVIRPGQERAEQHIDLAYAITAHGAQGASETFAIALEGTEGNRKLMAGFESAYVAL
                     SRMKQHVQVYTDNRQGWTDAINNAVQKGTAHDVLEPKPDREVMNAQRLFSTARELRDV
                     AAGRAVLRQAGLAGGDSPARFIAPGRKYPQPYVALPAFDRNGKSAGIWLNPLTTDDGN
                     GLRGFSGEGRVKGSGDAQFVALQGSRNGESLLADNMQDGVRIARDNPDSGVVVRIAGE
                     GRPWNPGAITGGRVWGDIPDNSVQPGAGNGEPVTAEVLAQRQAEEAIRRETERRADEI
                     VRKMAENKPDLPDGKTELAVRDIAGQERDRSAISERETALPESVLRESQREREAVREV
                     ARENLLQERLQQMERDMVRDLQKEKTLGGD"
     gene            29459..30205
                     /gene="traX"
                     /locus_tag="NBI74_002069"
     CDS             29459..30205
                     /gene="traX"
                     /locus_tag="NBI74_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus acetylase TraX"
                     /protein_id="HCD1932412.1"
                     /translation="MTTDNTNTTRNDSLAARTDTWLQSFLVWSPGQRDIIKTVALVLM
                     VLDHINLIFQLKQEWMFLAGRGAFPLFALVWGLNLSRHAHIRQPAINRLWGWGIIAQF
                     AYYLAGFPWYEGNILFAFAVAAQVLTWCETRSGWRTAAAILLMALWGPLSGTSYGIAG
                     LLMLAVSYRLYRAEDRAERLALLACLLAVIPALNLASSDAAAVAGLVMTVLTVGLVSC
                     AGKSLPRFWPGDFFPVFYACHLAVLGVLAL"
     gene            30264..31124
                     /locus_tag="NBI74_002070"
     CDS             30264..31124
                     /locus_tag="NBI74_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCD1932413.1"
                     /translation="MKITDHKLSEGIALTFRVPEGNIKHPLIILCHGLCGIRNVLLPC
                     FANAFTEAGFATITFDYRGFGESDGERGRLVPAMQTEDIISVINWAEKQECIDNQRIG
                     LWGTSLGGGHVFSAAAQDQRVKCIVSQLAFADGDVLVTGEMNESERASFLSTLNKMAE
                     KKKNTGKEMFVGVTRVLSDNESKVFFEKVKGQYPEMDIKIPFLTVMETLQYKPAESAA
                     KVQCPVLIVIAGQDSVNPPEQGKALYDAVASGTKELYEEADACHYDIYEGAFFERVAA
                     VQTQWFKKHL"
     gene            31227..31787
                     /locus_tag="NBI74_002071"
     CDS             31227..31787
                     /locus_tag="NBI74_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fertility inhibition protein FinO"
                     /protein_id="HCD1932414.1"
                     /translation="MTEQKRPVLTLKRKTEGETPTRSRKTIINVTTPPKWKVKKQKLA
                     EKAAREAELTAKKAQARQALSIYLNLPSLDEAVNTLKPWWPGLFDGDTPRLLACGIRD
                     VLLEDVAQRNIPLSHKKLRRALKAITRSESYLCAMKAGACRYDTEGYVTEHISQEEEV
                     YAAERLDKIRRQNRIKAELQAVLDEQ"
     gene            31916..32128
                     /locus_tag="NBI74_002072"
     CDS             31916..32128
                     /locus_tag="NBI74_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001369432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANR family transcriptional regulator"
                     /protein_id="HCD1932415.1"
                     /translation="MNGTGRDSQNSGVWRYRQVGRRAVRLEVRGDWPGAAEAWLRAAQ
                     SAPRTDWQQFARKRAEHCHRRCRGRV"
BASE COUNT         7995 a         7628 c         9285 g         7368 t
ORIGIN      
        1 attttggcgt gttttttttc gtcggtttct gtgcaatcat gattttatta gtcgggaaac
       61 agaggtgaat tgagtgagga ggcgtaacgc gagaggcggg ataagcagaa cagtctcagt
      121 gtatctcgat gaagatacaa ataaccgact tattcgggcc aaagatcgta gcggtagaag
      181 taaaactata gaggttcaga tccgtctcag agatcatctg aaacggtttc ctgatttcta
      241 taatgaagaa atattcaggg aggtgatcga agagaacgag tcaacattta aagaacttta
      301 gtttttatct atcaatatta aacagaggaa tcatatgaat actgttttaa gtgttcaggg
      361 tgcttctgcg cccgtcaaaa agaaatcgtt tttttctaaa ttcactcgtc tgaatatgct
      421 tcgcctggct cgcgctgtga tccctgctgc tgttctgatg atgttcttcc cgcaactggc
      481 aatggcagca cagggccagg acctgatggc aagcggtaac accacggtta aggcgacctt
      541 cggtaaggac tccagtgttg ttaaatgggt tgttctggct gaagttctgg tcggtgcggt
      601 catgtacatg atgaccaaaa acgtcaagtt cctggccggt tttgccatca tctctgtatt
      661 tattgctgtg ggtatggccg tcgttggcct gaaataaaat gatgtcggga gacgagaata
      721 aacttaagaa atatcgtttc ccggaaacac tgaccaacca gagccgctgg tttggcctgc
      781 cactggatga actgatcccc gcagcaatct gtattggctg gggtatcaca acatcgaaat
      841 atctgttcgg tattggtgca gcggttctgg tttatttcgg gattaaaaaa ctgaaaaaag
      901 ggcggggcag ttcctggtta cgtgacctga tttactggta tatgccaaca gccctgctgc
      961 gcggtatttt tcataatgtt cccgattcgt gtttccggca gtggattaaa tagaactgat
     1021 accaggattg ttatatggaa cacggtgccc gtttaagtac cagtcgtgta atggccatcg
     1081 cctttatatt tatgtcagtg cttattgttc tcagcctctc tgttaacgtt attcaggggg
     1141 tgaataacta ccgtcttcag aatgagcaac gcactgccgt gaccccaatg ggatttaatg
     1201 tgccctttgc cgtgtcacag aacagtgccg acgcctctta tttacagcag atggcgctgt
     1261 catttattgc cctccgtctg aatgtttcat cagaaaccgt cgatgcctca catcaggcgc
     1321 ttctgcaata tatccgcccg ggcgcacaga accagatgaa agttattctg gctgaagaag
     1381 cgaagcgtat taaaaacgat aacgtgaact cagccttttt ccagaccagt gttcgtgtct
     1441 ggcctcagta tggccgtgtg gaaattcatg gtgtgcttaa aacctggatt ggtgattcaa
     1501 aacctttcac tgatatcaaa cattacatcc ttattctgaa gcgggaaaac ggggtgacct
     1561 ggctggataa tttcggggaa acagacgatg agaaaaaata atacggcaat aatattcggc
     1621 agcctgtttt tttcctgcag cgtgatggcc gcaaacggta cgctggcccc cactgtggtg
     1681 ccaatggtga acggtggtca ggccagtatt gccatcagca ataccagccc gaatctgttt
     1741 accgttcccg gtgaccggat tatcgccgtg aacagtctgg atggtgccct gaccaataat
     1801 gagcagaccg cctccggcgg tgtggtggtt gccaccgtca acaaaaagcc ctttacgttc
     1861 attctggaaa cagaacgtgg tctgaatctt tccattcagg ccgttccccg tgaaggcgcg
     1921 gggcgtacca ttcagctggt cagtgacctg cgcggaaccg gagaagaagc cggtgcgtgg
     1981 gaaacgtcca cgccttacga atccctgctt gtgaccatca gccaggctgt ccgtggcgga
     2041 aaattacctg caggctggta tcaggttcct gtgacaaagg aaaccctgca ggccccggcg
     2101 gggctgtctt cagtggcaga tgccgtatgg acggggaatc acctgaagat ggtccgcttt
     2161 gccgtggaaa ataaaacgct gtctgccctg aatatccggg aaagtgattt ctggcagccg
     2221 ggaacccgtg ccgtgatgtt cagccagcct gccagccagt tactggcagg tgcgcgcatg
     2281 gatgtgtatg tcatccgtga cggggagggc aactgatggc cagtatcaat accattgtga
     2341 aacgcaagca gtacctgtgg ctggggattg tggttgtcgg tgcagcctcc gcgattggtg
     2401 gtgcactgta tctgtctgat gtggacatgt ccggtaacgg tgaggcggtg gctgaacagg
     2461 agcctgtgcc ggatatgacc ggtgtggtgg atacgacctt tgatgacaag gtgcgtcagc
     2521 atgccaccac agagatgcag gtgacggcag cgcagatgca gaagcagtat gaggaaatcc
     2581 gtcgtgagct ggatgttctg aacaaacagc gcggtgatga ccagcgtcgt attgaaaagc
     2641 tgggacagga caatgccgcc ctggcagagc aggtaaaagc cctgggtgct aatcccgtca
     2701 cggcgaccgg tgagcctgta ccgcagatgc ctgcctcacc gcccggcccg gaaggcgaac
     2761 cacagccagg aaacaccccc gtatccttcc cgccgcaggg cagcgttgct gttccaccgc
     2821 cgacggcgtt ttatcccggg aatggtgtca cgccaccacc acaggtgacg taccagtctg
     2881 tgccggtgcc taaccggata cagcgtaagg tgtttacccg taatgaggga aaacagggac
     2941 catcactgcc gtacattccg tcaggaagtt ttgcgaaagc catgctgatt gaaggagcgg
     3001 atgccaatgc ctcagtcacc ggtaatgaat ccacggtgcc gatgcagctg cgtatcaccg
     3061 gtctggtgga aatgccgaac agcaaaacgt atgacgcaac gggatgtttt gtggggctgg
     3121 aagcctgggg ggatgtgtcc agtgaacgtg ccattgtacg cacccgtaat atcagctgcc
     3181 tgaagaatgg caaaaccatt gatatgccga ttaaggggca tgtcagcttc cggggtaaaa
     3241 acggtatcaa gggcgaagtg gtgatgcgta acggcaaaat cctcggctgg gcatggggcg
     3301 cgggatttgt tgacggtatc ggtcagggaa tggagcgcgc ctcccagccg gctgtcgggc
     3361 tgggtgccac agccgcttac ggggctggtg atgtcctgaa aatgggtatc ggtggcggcg
     3421 catcgaaagc cgcacagacg ctcagtgact actacatcaa acgtgccgaa cagtatcacc
     3481 cggtgatacc gattggtgcg ggcaacgaag tgaccgtggt gttccaggac ggcttccagc
     3541 tgaaaaccgt ggaagagatg gcgctggaac gcacgcagag cagagcggaa gaagagaatc
     3601 aggaaagtcc ggttcctgtt ccgccatcgg ctgaaagtca tcttaacggc tttaatactg
     3661 accagatgct gaagcagctg ggcaacctga atccgcagca gtttatgtcc ggcagccagg
     3721 gagggggcaa cgatggcaaa taatatgtcg tcccgtcagg cgtgccatgc cgcccggtat
     3781 gttgtcgccc gggttctgcg aggactgttc tggtgtctga agtacaccgt cattctgcca
     3841 ctggccacaa tggccctgat ggctctgttt gttttatgga aggacaacac cacgccaggg
     3901 cagttactgg ttcaggaaat tgacttcgta agacaaacag cccctgccgg gcagtttcct
     3961 gtaagagagt gtttgccttc tctgcatgat cttccaggag cagaagagaa aatctgccgt
     4021 taccgtgctg ctgatgctgc agattatgtc agggagactg accggtcact gatgcaactg
     4081 gttaccgcat tatgggcaac actggcattg atgtatgtct cactggcagt tatctccggg
     4141 aaatatccgg tgctgcccgg aaaaatgaaa tgtatccggg gggtaaccgc agatgaacgt
     4201 ctgacggaag tttatacgga agacgcttcc ctgccggaaa aaatcaggaa atgccctgtt
     4261 tattttcctg atgacagaac aaacagaaac aacggagata aaaatgaaca tgcgtaatat
     4321 taatgttatt actgcccgct ctgtaccgga taaaagcgta tcagatgatt ttatgcatgc
     4381 tgttctcagt aactgcacca caagaatcgt acttcctgca ccgaaggaat tcagttctga
     4441 atcattgccg cacaatttta atatggctgc tgtcggtgtg atgaagaagg gaatgtcgat
     4501 gagcttattt tcttctccgg ggcatattgt ggttgccgga caatccggtg gcggaaaatc
     4561 tgttccggtg aaagaactaa tcgacagaat tattcagaaa tataaggaac ccttcgatga
     4621 ataagctggt ttctgatggc agcgtcaaaa aaataaatta tccggtgctg tatgaatccg
     4681 gaataacacc tccgttatgt gaagtcagtg caccagaacc tgatgcgggg gggaaacgca
     4741 ttgtggcata tgtttataaa tcatcacgga atacggtttt tgaaaatcct gacattgtaa
     4801 aaacctgtac ggtcagggat ttgaagaatg attttgtgaa ctgcgatgag aaaggagagg
     4861 gacaatgaaa cagatttctt tatttattcc tctgctgggg accttattac tttccggctg
     4921 tgccggaacc agtacggaat ttgagtgtaa cgccaccaca tccgatacct gtatgacgat
     4981 ggagcaggcc aatgagaagg ccaaaaaact ggagcagtcc tcagaagcaa agccggttgc
     5041 ggcatcactg ccgcgcctgg ctgaagggaa cttccggaca atgccggtgc agaccatcac
     5101 cgcgaccact ccgtccggca gcagacctgc ggtgacggca cttccggagc agaaactgct
     5161 ggcaccacgt ccgttgttta cggctgcccg ggaagtgaaa acgattgttc cggtcagttc
     5221 agttgcgccg gtaacacctc cccgcccgct aagaacgggt gagcagacgg ctgcattatg
     5281 gatagcgcct tatattgata atcaggatgt ttatcatcag ccatccagtg tgttttttgt
     5341 tattaaaccg tctgcgtggg gaaaaccacg tattaattaa ctggccctga agttgtatat
     5401 cagccggcaa tgccggcggg ctttctgtat tcaaaatctg cctgtaaaac gagtgccctg
     5461 tgcgtgaaaa gggatgggcg gtagcgtgtc agaacatgtc gggaggaata ttccgtgagt
     5521 gatgaagtcg atgaagcata ttcagtaaca gaacaactga ccatgacagg aataaaccgg
     5581 atacgccaga aaataaatgc tcatggtatt cctgtttatc tctgtgaagc atgcggtaat
     5641 cctattccgg aagcccggcg gaaaatattt cccggtgtga cgttatgtgt tgaatgtcag
     5701 gcgtatcagg aaaggcagag aaaacattat gcataagtca gtcgcagaac atattgattt
     5761 aattccggat gaacatgagt tgatattcag aaaaccgaaa tctttgtata tgcgccggga
     5821 aatggcgcgt taattacagg tattcccttc atggctaccg catttctcgc tttatttttc
     5881 aactaaggaa ttcatgtgaa taacccactt gaggccgtca ctcaggcggt taactccctc
     5941 gtcacagcac tgaaactgcc tgacgaatcc gcaaaggcca atgaagttct gggcgaaatg
     6001 agcttcccgc agttcagccg tctgctgccg taccgtgatt acaaccagga gtccggcctg
     6061 ttcatgaatg acaccacgat gggctttatg ctggaagcca ttcccatcaa tggtgcgaat
     6121 gagtccattg tggaggcgct cgatcatatg ctgcgcacca aactgccgcg cggtattccg
     6181 ttgtgtatcc atctgatgtc cagtcagctg gttggtgaca ggattgaata cgggctgcgt
     6241 gagttctcct ggtcaggtga acaggctgaa cggtttaacg ccattacccg tgcctattat
     6301 atgaaagcgg cagcgacaca gttcccgctg ccggagggga tgaatctgcc cctgaccctg
     6361 cgtcattacc gggtgtttat ctcgtactgt tctccctcga agaaaaaaag ccgggccgac
     6421 attctggaaa tggaaaatct ggtgaaaatc atccgtgcct cttttcacgg cgcaaaaatc
     6481 accacacaga cagtggatgc acaggctttc atcgagattg tcggggagat gattaaccat
     6541 aatccggact ctctgtaccc gaaaagacgt cagctggacc cgtattctga tctgaattat
     6601 cagtgtgtgg aggacagttt tgatctgaag gtccgggcgg attacctgac gctggggctg
     6661 cgtgagaacg gcaggaacag cacagcccgt atcctgaatt tccatctggc ccgtaacccg
     6721 gaaatcgcct tcctgtggaa catggccgac aactacagca atctgctgaa cccggaaatg
     6781 tccatttcct gcccgttcat cctgacgctg acactggtgg tggaagatca ggtgaaaacc
     6841 cacagtgaag ccaacctgaa gtacatggac ctggagaaaa agtcgaagac ctcctatgcc
     6901 aaatggtttc cgtccgtgga gaaagaagct aaggagtggg gggaactgcg tcagcggctg
     6961 ggctccggtc agtcttctgt cgtgtcctac ttccttaaca tcacagcatt ctgcaaggac
     7021 aacaatgaaa cggcgctgga agtggagcag gacattctga acagctttcg taaaaacggt
     7081 tttgagctga tttcaccgcg ctttaaccac atgcgcaatt tcctgacctg cctgcccttt
     7141 atggccggga aagggctgtt taaacagctg aaagaggccg gtgtggttca gcgagcagag
     7201 agctttaatg tggccaacct gatgccgtta gtggcggata accccctgac accggcaggc
     7261 ctgctggcac ccacctaccg taaccagctg gcgtttatcg atatcttctt taaggggatg
     7321 aataacacca actacaacat ggcggtctgt ggaacatccg gggccggtaa aaccgggttg
     7381 atacagccac ttatccgcag cgtgctggac tccgggggct ttgccgtggt gttcgacatg
     7441 ggggatggct acaagtcttt gtgtgagaac atggggggcg tatatctgga cggtgaaacc
     7501 ctgcgcttta acccgtttgc gaacatcact gatattgacc agtcagcgga gcgtgtccgt
     7561 gaccagttgt cggtgatggc cagccccaac ggtaacctgg atgaagtgca tgaaggtctg
     7621 ttgctgcagg cggtcagagc ctcctggctg gctaaaaaga agcaggcgcg cattgatgat
     7681 gtggtggatt tcctgaaaaa cgcccgtgat aacgaccagt atgtcgagtc accgaccatc
     7741 cgcagccgtc tggacgaaat gattgtgctg cttgaccagt acactgccaa cggcacttac
     7801 ggccggtatt ttaactctga tgagccgtcc ctgcgggatg atgccagaat ggtggtgctg
     7861 gagctgggcg gactggaaga ccgtccgtca ctgctggttg cggtgatgtt cagccttatc
     7921 atttacatcg agaaccggat gtaccgcacg ccgcgtaccc tcaagaaact gaacgtcatt
     7981 gatgaaggct ggcgtctgct ggacttcaaa aatcgtaaag ttggtgagtt tattcaaaaa
     8041 ggttaccgca cctgtcgccg tcataccggt gcctatatca ccatcacaca gaacatcgtc
     8101 gactttgact ctgacaaggc atccagcgca gcccgtgcgg catggggtaa ctcctcctac
     8161 aaaattatcc tcaaacagag tgcgaaggag ttcgcgaaat ataaccagtt attcccggat
     8221 cagttccagc cactgcagcg cgacatgatt ggtaagtttg gtgcggccaa agaccagtgg
     8281 ttcagttcct tcctgctgca ggtggaaaac cattcctcct ggcaccgtct gtttgtggac
     8341 ccgttaagcc gtgccatgta cagctctgac ggcccggatt ttgagtttgt gcagcagaaa
     8401 cgcagagagg gtatgagtat tcatgaggcg gtgtggcagc tggcgtggaa gaaatcgggg
     8461 ccggagatgg cttcgctgga ggcctggctg gaagaacatg agaaatacag gagtgttgca
     8521 tgaccacaac gcaaaaaacg actgacgtta cagccccccg acgcagccac tggtggtgga
     8581 ccgtgccggg atgcctggca atggtgttac tgaacgcagc catcagttat ggcattgtca
     8641 gactgaacgc accggtgact gccgccttca acatgaaaca gaccgtggat gcgttttttg
     8701 acagcgccag tcagaaacaa ctgtcagaag cacagtcaaa agccctttca gcgcgtttta
     8761 acacggcact ggaagccagc ctgcaggcat ggcagcagaa acaccacgcg gtcattctgg
     8821 tgtcgcctgc cgtggtacag ggggcaccgg atatcacccg ggaaatccag caggatattg
     8881 cccggcgaat gagggcggaa ccatgagatg ccgggggctg attgccctgc tgatatgggg
     8941 gcagagtgtg gccgccgccg atcttggcac ctggggcgat ctgtggccgg taaaggagcc
     9001 ggacatgctg acggtgatca tgcagcgcct gacggcactg gagcagtccg gtgagatggg
     9061 ccggaaaatg gatgccttta aggagcgggt gatccgtaac agtctgcggc cccctgccgt
     9121 gcccggtatc ggacgcacag agaaatacgg cagccggttg tttgacccgt ccgtcagact
     9181 ggctgcggat atccgggata acgaagggcg ggtgtttgcc cgtcagggtg aagtgatgaa
     9241 tccgctgcag tatgttcctt ttaaccagac gctgtatttc atcaatggcg atgatccggc
     9301 gcaggtggcc tggatgaaac gccagacgcc gcccacactg gagagcaaaa ttatcctcgt
     9361 gcagggcagt atcccggaga tgcagaagtc tctggacagc cgtgtttact ttgaccagaa
     9421 cggtgtgctc tgtcagcgcc tcggcattga tcaggtcccg gcacgggtga gcgccgttcc
     9481 cggcgatcgc ttcctgaagg tggaatttat tccggcagag gagggcagaa aatgaagcga
     9541 agcctgtggc tgctgatgtt attccttctc gccggtcatg tccctgcagc gtctgcggat
     9601 tctgcctgtg aggggcgttt tgtaaacccg atcacggata tctgctggag ctgtattttc
     9661 ccgctctcgc tgggcagtat caaagtcagt cagggtaagg tccccgacac ggcgaacccg
     9721 tcgatgccca ttcagatttg tccggcaccg ccgccgctgt tcaggcgtat cgggctggcc
     9781 attggttact gggagccgat ggcgctgacg gacgtcaccc ggtcaccggg atgcatggtg
     9841 aacctgggct tcagcctgcc ggcttttggt aaaacggcac agggaacggc gaaaaaggat
     9901 gagaagcagg tgaacggggc gttctatcac gttcactggt acaaataccc gctgacgtac
     9961 tggctgaaca tcatcacatc gctgggctgt ctggaaggtg gtgacctgga tatcgcttat
    10021 ctttctgaaa tcgaccccac ctggacggac agcagcctta ccaccattct caacccggaa
    10081 gccgtcattt ttgccaatcc gatagcacag ggggcctgcg cagcagatgc cattgccagt
    10141 gcctttaata tgcctctcga tgttctgttc tggtgtgccg gttcgcaggg cagtatgtac
    10201 ccgttcaatg gctgggtgag taatgagtcc agtccgttgc agtcctccct gctggtcagt
    10261 gaacgcatgg cattcaagct gcaccgtcag ggcatgatta tggaaaccat cgggaaaaat
    10321 aacgccgtct gtaatgaata tccgtcccca atcctgccca aagaacgctg gcgttatcag
    10381 atggtgaata tgtatccgga cagcggacag tgccatccgt tcgggcgcag tgtgatgcgc
    10441 tgggagacgg gaaaaaatcc gcccaacaca aagaaaaact tcggctacct gatgtggcgt
    10501 aaacgtaact gtgtattcct gtaaatcatt ttaatgaata agaggaaatg aaaatggcaa
    10561 caatggacag taaatatatt cagcacctca ggaaaatagg tgctgtgctg ctcaggctga
    10621 taagcagaac attaaaatta tttccttttg gtcttgtaat agtgcccgtt atattatttt
    10681 attttgcacc tgacagtttt ctcgctgaaa ttattcatga atggaagaag gcagatatat
    10741 cagaacaagt gacacttgtc agattatatc tgaaggtgac atttacatgt gtgtttatgg
    10801 ctgtttttct caattatctt ttttctgatg agttcatgcc tgtaaatagc agaaagtgag
    10861 aaatatcatg aaccggaaaa tatttgcact ttttgctctg acagcaggga tgcatcttgc
    10921 tgttcatgct tcagaaaatg tgaacacaac tgaaaaccgc cagttcctga agcagcagga
    10981 aaatttaagc agacaactgc gagagaaacc tgaccatcag ctgaaagcct gggcggagaa
    11041 acaggtgctg gaaaaccccc ttcagcgttc agataaccat tttctggatg agctggttcg
    11101 taaacagcag gcttcgcagg acgggaaacc ccggcagggt gccctgtatt ttgtgtcgtt
    11161 ttccataccc gaagaggggc tgaaaaggat gctgagcgaa acccggcact acggtattcc
    11221 cgccacgctg cggggcatgg tgaacaacga cctgaaggcc acggctgacg ccgtgttgtc
    11281 cctggtgaaa gacggcgcaa ctgatggtgt tcagatcgac ccgacgctgt tttcacagta
    11341 cggcattcgc agcgttccgg ccctggtggt gttctgcagc caggggcacg acatcatccg
    11401 gggaaatctg cgcgtcaggc aggcgctgga gaaagtggcc gccacgggtg actgccggca
    11461 ggtggctcat gatttactgg cagggaaagg agattccggg aaatgaaacg tattttacct
    11521 ctgatactgg ctctggttgc cggcatggca caggctgaca gcaacagtga ttaccgggcc
    11581 ggttctgatt ttgcccgtca gattcagggg cagggtacag gcagtattca gagctttaag
    11641 ccacaggaga gtatccccgg ctataacgcg aatccggacg agacaaaata ttacggtggc
    11701 gtgactgccg gcggggatgg cggcctgaaa aatgacggca ccacggaatg ggcgaccggt
    11761 gaaaccggaa aaaccatcac agagtccttc atgaacaagc cgaaagacat tctgtcaccg
    11821 gatgcaccgt tcattcagac cggcagggat gtggtgaacc gggctgacag cattgtggga
    11881 aacaccgggc agcagtgcag tgcgcaggag attagcagga gtgaatacac gaattacacc
    11941 tgtgagcggg atttacaggt ggagcagtac tgcacacgaa ctgcccgtat ggagctgcag
    12001 gggagtacca catgggaaac ccggacgctg gagtatgaaa tgagtcagct acctgcacgt
    12061 gaagtgaatg gtcagtatgt tgtctccatg acctctcccg ttaccggtga aattgtcgat
    12121 gcgcattaca gctggagtcg tacttacctg cagaagagtg tacctatgac aattacagta
    12181 ctgggaaccc cactgagctg gaatgccaaa tactcagcag atgcctcatt tacaccagta
    12241 cagaaaacac tgactgccgg tgtggctttt acgtcatctc accccgtccg ggtcgggaat
    12301 acaaaattca gacgtcatac ggcaatgaag ctgcgtctgg ttgtcagggt aaaaaaagcc
    12361 tcgtacaccc cgtatgttgt ctggtctgaa agctgcccgt tcagcaagga gctggggaaa
    12421 ctgacaaaaa cagaatgtac ggaggctggc gggaacagga cgctggtgaa agacggtcag
    12481 tcatacagca tgtaccagag ctgctgggca taccgggaca cgtatgtcac acagtcagca
    12541 gacaagggaa cctgtcagac ctacaccgac aatccggcct gtaccctggt gtcacaccag
    12601 tgcgcctttt actccgaaga aggtgcctgt ctgcatgaat atgccacgta ctcctgtgag
    12661 tcaaaaacat ccgggaaagt gatgctctgt gggggagatg tcttctgcct cgatggcgaa
    12721 tgcgataagg ctcagagcgg gaagagtaat gattttgccg aagctgtttc tcagctggca
    12781 gcactggccg ctgcaggtaa ggatgttgcg gcgctgaacg gggtggatgt ccgtgctttc
    12841 accggtcagg cgaaattttg taagaaggct gcagccggat acagcaactg ctgtaaggac
    12901 agcggctggg gacaggatat cgggctggcc aaatgcagca gtgatgaaaa agccctggct
    12961 aaagcaaaat caaacaaact gaccgtcagc gtcggggagt tctgttcgaa gaaagtgctg
    13021 ggtgtctgtc tggagaaaaa acgcagctac tgtcagtttg attcgaagct ggcgcagata
    13081 gtccagcagc aggggcgcaa cggtcagctg cgtatcagtt ttggccgtgc gaagcatcct
    13141 gactgtcggg ggattacggt tgatgagctg cagaaaattc agttcgacag actggatttc
    13201 actaacttct acgaagacct gatgaataac cagaaaatcc cggacagtgg cgttctgacg
    13261 cagaaagtga aagagcagat tgctgaccag ctgaaacagg caggacagta aggtatggaa
    13321 aataaccggg ggaaatgatg aaagtcatat tcacgtcgaa ccgttttatt gattttctta
    13381 tccggctttt gattacagcg attgtcattt ccccggtaat tatctggtcg tgggatacgg
    13441 taaaagaaac cacggcagac gggatgcttg ccgctgcttt cgttatcctt tactccggtg
    13501 tgctcctgtt tattctgtat ttttgttttt ctgccctgac tggcctgcag aaagctgatg
    13561 aaagaaaaaa tgatgaaagg aatgaagatg aataaagcat tactgccact gttactctgc
    13621 tgctttattt ttccggcgtc agggaaagat gcaggctggc agtggtataa cgagaaaata
    13681 aatccgaagg aaaaagaaaa taaacctgta cctgcagccc cccgtcagga gccggatatt
    13741 atgcagaaac tggccgcgct gcagacggca acaaagcggg cgctgtacga agccattctg
    13801 tatcccggcg tggataattt tgtgaaatat ttccggctgc agaattactg gactcagcag
    13861 gccgggcttt tcaccatgag tgctaaaaag gccatgctgg cacatcccga actggactat
    13921 aacctgcagt acagccatta caacggcacg gtacggaacc agctggcggc agaccaggcg
    13981 cagcagcgac aggccattgc gaaactggct gaacactacg gcatcatgtt tttttaccgg
    14041 gggcaggacc ccatcgacgg gcagctggcg caggtcatta atggcttccg ggatacgtat
    14101 ggtctgagtg ttattcccgt ttccgtggat ggcgtgatta atccgctgtt gccggattcc
    14161 cggactgacc aggggcaggc gcagcgcctc ggcgtgaaat atttcccggc catgatgctg
    14221 gttgacccga aacagggcag tgttcgcccg ttatcatacg gctttatttc gcaggacgac
    14281 ctggcaaaac aattcctgaa cgtttctgaa gattttaaac caaattttta atcgcggatt
    14341 tgatttatga gtgaagatta tttgaaaatg tttacaggtg ttgttctgtt aatatttgtc
    14401 attattgccg gttatttctt ttctgagcgt aatgacagga aaatgtttct cctgagttca
    14461 ctggttttcc ttgttattaa tatcgcgtgt ttatatgtgc ttaccgccag tctctggttt
    14521 ctgtgtggtg caattatgaa tcagggcgca gcaatggttg ttccaatggt tattggctca
    14581 ttaccgaacg ttacgagctt cgacaggttc agaagaatat ttatctgtat tatgttgtca
    14641 tcggcatggt ccggagtgat gtggtttttt ataaggggct tatgacaggc taagtcatat
    14701 aaaacggtaa taaaaaccat acaaaataat gaggttgatc tgattaatat cacacaaacg
    14761 cgaatattac agacatgaat attctcatgt aaataataac cggagtttaa catgataagt
    14821 aaatgcagat tctctttacc ccggcttgat attacgggaa tgtgggtatt ttctctgggt
    14881 gtctggtttc atatcgtcgc ccgtcttgtt tacagcaaac cctggatggc tttttttctg
    14941 gcggagttga ttgcggccat ccttgtgctg ttcggcgcat accaggtgct ggatgcatgg
    15001 attgcccgtg tcagccggga agagcgtgag gcactggaag ccaggcagca ggccatgatg
    15061 gaagggcagc aggagggcgg acatgtctct cactaaatta ctgccggtca ttttgttgat
    15121 gatggccaca ggtgtgcagg cctccacccg ggatgaaatt gaacgactct ggaatccgca
    15181 gggtatggcc gtgccgcctg cacaaccggc agcagacaca tcagccagaa cagaaaagcc
    15241 ggctccccgc tggttccgcc tcagcaacgg caggcaggtt aacctggccg actggaaggt
    15301 ggtgctgttt atgcaggggc gttgccctta ctgtcaccag tttgacccgg tactgaagca
    15361 gctggcacag cagtacggct tttccgtttt tccctacacc ctggatggtc agggggatac
    15421 tgcctttccg gaagcattac cggtgccacc ggacgtgatg cagaccttct tcccgaatat
    15481 cccggtggcc acaccgacca ccttcctggt caacgtcaac acacttgagg cattaccgct
    15541 tttgcagggg gcaacggatg ccgccagttt tatggcgcgg atggataccg ttctgcagat
    15601 atacggagag gaaaaaggtg cgaaataagc aggtggtgtt actcattgcc ggaatatccg
    15661 gcatcgcgac gggaataatc gtcagcctga atatcccctt tatccgtcag gggctttttt
    15721 atcccgccag tcctgtggaa attgtcgttt cgctgtgtct taccttttct gtttctgttg
    15781 tgttttttgt gggggcaatt gtgggatgga tttcggtgtc atattacagc cgcataacct
    15841 gtctgaatga atcatcagaa atatcagagg aaacttataa tgaaagaaaa aagaaatgat
    15901 gcggaactaa aaaacagaaa aactaaacgg aattatgatt atgaacgcag ggtttctgat
    15961 atatattttg atctgttttt tgtgttcgtt gctgccggaa cttttttatg ggtgattatg
    16021 cacagtattt ttgatgcctg tattgattcg tggaaagctg acccagcatt aaataatttt
    16081 cgttatatgt ggaacatcct gatgtatgtc attccgtata cgttatgggc gtttgcaggt
    16141 ggatttctta tcgtatacgt caggaaccct ttaaatgaat taataaacgg ggggatcagg
    16201 atattccggc tgaaacgccg tatgcgccgg gagaacagtt tcagggaggg gaataacgat
    16261 gcctcgcatt aaacctcttc tggttctttg tgccgtttta ctgacggtca cacccgcagc
    16321 gtcagcggat gtgaacagcg acatgaatca gttctttaat aagctgggat ttgcatccaa
    16381 tgtcacccag gcgggcgtct ggcaggggca ggccgccggg tatgcctatg gtggctccct
    16441 gtatgcccgc acccaggtca aaaatatcca gctgatttcc atgacgctgc cggatatcaa
    16501 tgccggatgt ggcggcatcg acgcctatct cggctcgttc agttttatta atggcgatca
    16561 gttgcagcgt tttgttaagc agattatgag caatgctgcc ggttactttt ttgaccttgc
    16621 cctgcagaca acggtgccgg aaataaaaac cgcaaaagac ttcctgcaga aaatggcaag
    16681 tgacattaac agtatgaacc tcagttcctg tcaggtggca caggggcttg tgggggggct
    16741 gttccctcag acgcaggaat ctcagcagaa agtttgtcag gacattggca gtgaaagcaa
    16801 tatcttcgcc gactgggccg catctcgtca ggggtgcacg gtcggagggc agtcctcatc
    16861 tgttcgtgac aaagccagcg acaaggacaa ggagcgggtg ctgaaaaacc agaatatcat
    16921 atgggaggcg ctgaaaaaaa acagaatgtt tgacggcaac aaagagttga aagaatttat
    16981 catgacgctc accggtacgc tgatttttgg ggaagacagc gagattaccc ccctgccggc
    17041 gcgtaccact gaccgcgata tcctccgggc gattatggag gggggaacgg caaaaattta
    17101 ccactgcaac gattcggaca agtgtctgaa ggtggtggcg gatgcaaatg tgaccatcgc
    17161 ccgggataaa gccctgaagt cacagattac aaaactgctg accagtattc agaataaagc
    17221 agtgagtgac actccgcttg acgcccggga acaggggttt atttccagca ccaccattcc
    17281 cgtttttaaa tatctgattg acccgcagat gctcggtgtg tccaacagcg tcgtttacca
    17341 gctgacggac tatatcggct acgacatcat gctccagtac attcaggaac tgcttcagca
    17401 ggcccgggcc atgattgcca ccgggaacta tccgcaggct gtgatggaca atgttctgga
    17461 gaatctgaat caggcacagc aacagattgc cattttccag agtcaggtac aggtccagca
    17521 ggatgcgctg ctggttgtcg aacgtcagat gagctacatg cgtcagcagc tttccgcccg
    17581 tatgctcagc cgttaccaga acaactatca cttcggaggg aattcactgt gaatgaagtt
    17641 tatgtgattg ccggtggtga gtggttgcgt aacaacctga atgccattgc agcatttatg
    17701 aatacccgga cctgggattc aattgaaaaa atcgcgctca ccctgtctgt tgttgctgtg
    17761 gcttttatgt gggtacagcg gcataacgtg atggatttgc tgggctgggt ggccgtgttt
    17821 gtgcttatca gcctgctggt taatgtccgt acatcggtgc agattattga taacagtgac
    17881 ctggtcaaag tccatcgggt ggataatgtg ccggtcggtc tggcgatgcc actttcactg
    17941 acgacacgta tcgggcatgc aatggtggtc agctacgaga tgatattcac ccaaccggac
    18001 agcgtcacct acagcaagac aggcatgctg ttcggggcga atctgattgt gaaaagcacc
    18061 gatttcctgt cccggaatcc ggaaatcatc aatctgttcc aggactacgt ccagaactgc
    18121 gtgctgggtg atatttacct gaatcataaa tacacgctgg aagatctgat ggcctccgct
    18181 gacccctaca cgctgatttt ttcccgaccc agcccgctac ggggcgttta tgacaacaat
    18241 aataatttca taacctgtaa ggatgcgtcg gtcacgctga aagacaggct gaatctcgat
    18301 acaaagacgg gaggcaagac ctggcattat tatgtgcagc agatatttgg cggcagaccg
    18361 gacccggacc tgttattcag acaactggtc agtgacagtt acagttattt ctacggctcc
    18421 agtcagtctg ccagccagat tatgcgccag aacgtcacaa taaatgccct gaaagagggt
    18481 atcaccagta atgccgcccg taatggtgac accgccagcc tggtgaatct ggccaccacg
    18541 tcatcgatgg agaagcaacg tctggcgcat gtctctatcg gtcatgtgac aatgcgaaac
    18601 ctgccaatgg tacagactat cctgacggga attgccattg gtattttccc gcttctggta
    18661 ctggcagctg tcttcaacaa actgacactg tccgtcctga aaggctatgt gtttgccctg
    18721 atgtggctgc agacctggcc gttgttgtat gccattctga acagtgccat gacattctat
    18781 gcgaagatga atggtgcgcc ggtcgtgctg tctgaactct ctcagataca gctgaaatac
    18841 tctgacctgg cctctactgc cgggtatctt tcagccatga tccccccgtt gtcatggatg
    18901 atggtaaaag gccttggcgc aggtttttcc agcgtgtaca gccacttcgc ctcttcctct
    18961 atcagtccga cggccagtgc agcaggcagt gtggttgacg gtaattactc ctacggcaac
    19021 atgcagacgg aaaacgtgaa cggcttcagc tggagcacca acagcaccac gtcgtttggt
    19081 cagatgatgt accagaccgg cagtggcgca actgccacac agacccgtga cggtaatatg
    19141 gtgatggatg caagcggagc gatgtcccgg ttaccggttg gtatcaatgc aacgcgtcag
    19201 attgcagcgg cacaacagga aatggcccgg gaggcgtcga acagagcaga aagtgccctg
    19261 catggtttca gcagcagtat tgccagtgcc tggaactcgc tcagtcagtt tggttctaac
    19321 cgggggagca gtgattctgt caccggtggt gctgacagta cgatgagcgc acaggactcc
    19381 atgatggcca gccgtatgcg cagtgcagtg gaaagctatg cgaaggcgca taatatcagt
    19441 aatgagcagg cgactcagga actggcatcc agaagtacga gagcttctgc cgggatgtat
    19501 ggagatgctc atgctgaatg gggggttaaa cctaaaattc ttggtgttgg tggaggatta
    19561 ggcgttagag gcggtggtcg ggctggcatt gactggagtg atgatgatgc gcatcaggcc
    19621 agcagtggtt cacgggccag caatgatgct cgtcatgata tcgatgccag agctacacaa
    19681 gactttaaag aggccagcga ttactttacc agtcgcaagg tcagtgagtc cggcagccat
    19741 actgacaata atgctgattc ccgtgtggat cagttgtctg cagccctgaa ttcagcgaag
    19801 cagagttacg accagtacac gacgaatatg acccgcagcc atgaatatgc tgaaatggca
    19861 tcccgtactg aaagcatgag tgggcagatg agtgaagact tgtcgcaaca gtttgcacag
    19921 tatgtaatga aacacgcgcc acaggatgcc gaagctattc tgacaaacac cagttcaccg
    19981 gagattgcag aacgtcgtcg ggcgatggcc tggtcttttg tgcaggaaca ggtacagcct
    20041 ggtgttgata atgcctggcg ggaatcccgt ggcgatattg gtaaaggaat ggagagcgta
    20101 ccttcgggtg gtggcagtca ggatattatt gctgaccatc aggaacatca ggccattatt
    20161 gagcaaagaa cgcaggacag taatatccgt aatgatgtaa aacatcaggt tgataatatg
    20221 gtcacagaat ataaagggaa tataggtgat actcagaaca gcatacgtgg cgaggaaaat
    20281 attgtaagag gacagtattc tgaactgcag aatcaccata aaacagaggc attatctcag
    20341 aataataaat ataatgaaga aaagtcggtt caggaaagaa tgcccggggc tgacagtcca
    20401 caggaactga tgaaaagggc aaaggagtat caggataagt ataagtaata atgctgtgtt
    20461 tgggagatga taatgagatg tttagctcat attgctctgg tgacggtgat acagttcatt
    20521 gcctgttatc tggctgattg ggggagtgcc gaaacagttt ttatcctgtg ctttattgta
    20581 ctatggcagg gattgtttat atggttgttt tctcaaatac gaaaaaaacg gcatgtgtcg
    20641 gatgagttta aattcagtaa aggcgtttgg tatataacga tgccagtatc ttccttactc
    20701 tctccgttat taagcctgat ggtttttatc attggtacac tatacgaatt aaggcgagtt
    20761 agtggttgca taagcataaa aaaatggggt caaaatcaac tcaaaaacca atatgatggg
    20821 agtgaaaagt tagacttcgg gggcattgaa cagcccccaa cgacctacta taacccttca
    20881 accggatacc ctatgcatgg aggctttgac tcagcgggaa atacttttgg gactcgttgg
    20941 caggattatt atgacaggca ataagaaaac tgtagtttgt aaaaaaggta atttgatgaa
    21001 aacaaaaaaa ttgatgatgg ttgcactggt cagttccact ctggcccttt cagggtgtgg
    21061 tgcgatgagc acagcaatca agaagcgtaa ccttgaggtg aagactcaga tgagtgagac
    21121 catctggctt gaacccgcca gcgaacgcac ggtatttctg cagatcaaaa acacgtctga
    21181 taaagacatg agtgggctgc agggcaaaat tgctgatgct gtgaaagcaa aaggatatca
    21241 ggtggtgact tctccggata aagcctacta ctggattcag gcgaatgtgc tgaaggccga
    21301 taagatggat ctgcgggagt ctcagggatg gctgaaccgt ggttatgaag gcgcagctgt
    21361 tggtgcagcg ttaggtgccg gtattaccgg atataactca aattctgccg gtgccacact
    21421 cggtgtgggc cttgctgccg gtctggtggg tatggctgca gatgcgatgg tggaagatgt
    21481 gaactatacc atgatcacgg atgtgcagat tgcagagcgt actaaggcaa cggtgacaac
    21541 ggataatgtt gccgccctgc gtcagggcac atccggtgcg aaaattcaga ccagtactga
    21601 aacaggtaac cagcataaat accagacccg tgtggtctca aatgcgaaca aggttaacct
    21661 gaaatttgaa gaggcgaagc ctgttctcga agaccagctg gccaaatcaa tcgcaaatat
    21721 tctctgatta tcttccggag gctggcctga ccggcctcct gactgacgcc tcgctttgct
    21781 cgttgtccaa ccccggaaag aaataaaata aattttattt ctccccggcg tttccgaaat
    21841 aaatatgaaa acactgagcg gttgtgcaaa ttctttgtgc gaccgtgaag tgttgccggc
    21901 gaagccggaa tttaacggtt attaatcttt tcatcattaa taatcagtcc gactgacttt
    21961 ttttcttcgg aatatcatca tgagttttaa cgcaaaggat atgacccagg gcggtcagat
    22021 tgcgtccatg cgtatccgca tgttcagcca gatcgccaat atcatgcttt actgcctgtt
    22081 tatttttttc tggatactcg ttggtctggt tttatgggta aaaataagct ggcagacgtt
    22141 tgtgaacggc tgtatttact ggtggtgtac cacgctggaa ggcatgcggg atttaatcaa
    22201 gtcccagcca gtatatgaga tccagtatta cggcaaaacc ttccggatga acgcggctca
    22261 ggtactgcat gataaataca tgatctggtg cggtgagcag ctatggtctg cattcgttct
    22321 ggcgtctgtc gtggccctgg ttatttgcct gatcaccttc tttgttgtct cctggattct
    22381 ggggcgtcag ggtaaacaac agagcgaaaa tgaagtcaca ggtggtcgtc agctgacaga
    22441 caatccgaaa gacgttgccc ggatgctgaa aaaagacggc aaggactccg atatccggat
    22501 tggcgacctg ccgattatcc gggattccga aatccagaac ttctgtctgc acggaacagt
    22561 gtcgacgggt aagtcagagg ttattcgacg tcttgccaac tatgcccgta aacgtggcga
    22621 catggtggtg atttatgacc gttcgtgtga gtttgttaaa agttactatg atccctccat
    22681 cgataagatc ctgaatccgc tggatgcccg ctgtgccgcc tgggatttgt ggaaggagtg
    22741 cctgacacag ccggattttg ataatgtcgc gaacacgctg atcccgatgg gaacgaaaga
    22801 ggacccgttc tggcagggat caggacgtac catttttgcg gaagcggcgt atctgatgcg
    22861 taatgacccc aaccgcagct acagcaaact ggtggacaca ctgctttcca tcaaaatcga
    22921 aaaactgcgt accttcctgc gtaattcacc ggcggccaac ctggtggaag agaaaattga
    22981 gaaaacggcg atttccatcc gtgctgtgct gaccaactac gtgaaagcca tccgttacct
    23041 gcaggggatt gagcataacg gtgaaccctt caccatacgt gactggatgc ggggtgtccg
    23101 ggaagatcag aaaaacggct ggctgtttat ctcgtcaaat gccgacactc atgcctccct
    23161 gaagccggtg gtttccatgt ggctgtccat tgccattcgt gggctgctgg caatgggaga
    23221 aaaccgtaac cgtcgtgtgt ggtttttctg tgatgagtta cccacgttac acaaactgcc
    23281 ggacctggtg gagatcctgc cggaagcccg taagttcgga ggctgttatg tgtttggtat
    23341 ccagtcctat gcccagctgg aagatatcta cggtgagaaa gcggcggcca cgctgtttga
    23401 tgtgctgaat acccgtgcct ttttccgctc cccgagccat cagattgcag agttcgcggc
    23461 cggtgagatt ggtgagaaag agcacctgaa agccagtctg cagtattcct acggtgctga
    23521 tcccgtgcgt gacgggatat caaccggtaa ggagatggag cgccagacgc tggtcagtta
    23581 ttccgacatt cagtctctgc cggatctgac ctgttatgtc actctgcccg gaccgtatcc
    23641 ggcagtaaaa ctctcactga aatatcaggc acgtccgaag gttgcccctg agtttattcc
    23701 gcgtgacatc aacccggaaa tggagaaccg tctgagtgcc gtacttgccg caagggaagc
    23761 agaaggtcgt cagatggcca gcctcttcga accggatgtg ccggaagttg tttccggaga
    23821 agacgtgact caggctgaac agccgcaaca gccgcaacag ccgcaacagc cgcaacagcc
    23881 gcaacagccg gtgtctcctg ccatcaacga taagaagtca gattcaggtg tgaatattcc
    23941 ggcagggggg atcgagcagg agctgaaaat gaaaccggaa gaagagatgg aacagcaact
    24001 gccacccggg atcagtgaat ccggtgaagt ggtggatatg gccgcttatg aggcatggca
    24061 acaggaaaat catccggaca tccagcagca gatgcagcgt cgtgaagagg tgaacattaa
    24121 tgtgcaccgg gagcgtgggg aggatgttga gccgggagat gatttctgat gatgagtatc
    24181 gcgcaggtca gatcggccgg aagtgccggt aactattaca ccgacaagga taattactat
    24241 gtactgggca gcatgggaga acgctgggcc ggcaaggggg ctgaacagct tgggctgcag
    24301 ggcagcgtcg ataaggatgt ttttacccgt cttctggagg gaaggctgcc ggacggagcg
    24361 gatctaagcc gcatgcagga tggcagtaat aagcatcgtc ccggctacga cctgaccttc
    24421 tccgccccca aaagtgtctc catgatggcc atgttaggtg gcgataagcg cctgattgat
    24481 gcacataacc aggccgtgga ctttgctgtt cgtcaggtgg aggcgctggc ctccacacgg
    24541 gtgatgacgg acggacagtc agaaacggtg ctgaccggta atctggtgat ggcactgttt
    24601 aaccacgaca ccagtcgcga tcaggaacca cagttacaca cgcatgcggt ggtggctaat
    24661 gtcacgcagc ataacggtga gtggaagaca ctgagcagtg acaaagtggg gaaaacgggg
    24721 ttcattgaga atgtgtacgc taatcagatt gcctttggca ggctctaccg ggaaaaactg
    24781 aaagagcagg ttgaggcgct gggctatgaa accgaagtgg tgggtaagca cggtatgtgg
    24841 gagatgccgg gcgtaccggt ggaggccttt tccggacgct cacaggctat ccgggaggcc
    24901 gtcggggaag acgcctcgct gaaatcccgg gatgtggcgg cactggatac gcgtaaatcc
    24961 aaacagcacg tcgatccgga aatcagaatg gctgaatgga tgcagacgct gaaggaaacc
    25021 gggttcgaca tccgggcata tcgtgacgcg gcggatcagc gtacggagat ccgcacgcag
    25081 gcgcccgggc cagcttcaca ggacgggccg gatgtgcagc aggcggtgac acaggcgatt
    25141 gccggattaa gtgaacgaaa agtgcagttc acgtacacgg acgtgctggc caggacggtc
    25201 ggcatactgc cgccggaaaa tggtgtgatt gaacgggcac gcgccggtat cgatgaggcc
    25261 atcagccgtg agcagcttat ccccctcgat cgtgagaagg ggctgttcac gtccgggatt
    25321 catgtgctcg atgagctgtc agtccgggca ctcagtcgtg acatcatgaa acagaaccgg
    25381 gtgaccgtac atccggagaa aagtgtcccc cggacggccg gttacagcga tgccgtcagt
    25441 gttctggcac aggatcgccc gtcgctggcc attgtgtccg ggcagggcgg tgcagccggg
    25501 cagcgtgagc gggtggctga actggtcatg atggcccggg aacaggggcg ggaggtgcag
    25561 attatcgctg ctgaccgtcg ctcgcagatg aacctgaagc aggatgaacg gctgtccggt
    25621 gaactgataa ccggacgtcg tcagctgctg gaaggcatgg ccttcacgcc gggcagtacc
    25681 gttatcgtcg accagggcga aaaactctcc ctgaaagaga cgttaaccct gctggatggt
    25741 gccgcacgtc ataacgtaca ggtcctgata accgacagcg ggcagcgaac cggtacaggc
    25801 agtgcgctga tggccatgaa ggatgccggg gtgaacacat atcgctggca gggcggagaa
    25861 cagcgaccgg ccaccatcat cagtgaaccg gaccgtaatg tccgctatgc ccggctggcc
    25921 ggagattttg cggccagcgt gaaagccgga gaagagagcg tggcacaggt cagcggggta
    25981 cgggaacagg ccatactgac acaggccatt cgcagtgagc tgaaaacgca gggcgtgctc
    26041 ggacaccctg aggtgaccat gactgcactg tcaccggtct ggctggacag ccggagccgt
    26101 tatctgcggg atatgtaccg accggggatg gtgatggagc agtggaaccc ggagacacgc
    26161 agtcatgacc gctatgtgat agaccgggtg acagcgcaga gtcacagcct gaccctgcgg
    26221 gatgcgcagg gcgaaacgca ggtggtgcgt atttcctccc tggacagcag ctggtcgctg
    26281 ttccggccgg aaaaaatgcc ggtggcagac ggcgagcgac tgagggtgac agggaaaatt
    26341 cccggactcc gcgtctccgg cggtgaccgc ctgcaggtgg catccgtcag tgaagatgcg
    26401 atgacggttg ttgtgccggg gcgggctgaa ccggcctccc tgcctgtgag cgattcaccg
    26461 ttcacggcac tgaagctgga gaacggctgg gtggaaacgc ccgggcattc cgtcagtgac
    26521 agtgcgacgg tgtttgcctc cgtcacacag atggcaatgg acaacgccac cctgaacggt
    26581 ctggcccgca gcggtcgtga tgtccggctg tattcctcac tggatgaaac ccgtactgcg
    26641 gaaaaacttg cccgccatcc ctcctttacg gtggtttctg agcagataaa ggcgcgggcc
    26701 ggtgagacat tgctggaaac cgctatcagt ctgcagaaag ccgggcttca cacgccggca
    26761 cagcaggcca ttcatctggc ccttcctgtg ctggaaagta aaaacctggc cttcagcatg
    26821 gtggacctgc tgacagaggc gaagtcgttt gctgcagaag gaaccggttt tactgaactg
    26881 ggaggggaaa tcaatgcgca gataaaacgc ggtgacttac tgtatgtgga tgtggcaaaa
    26941 ggctatggca caggcctgct ggtttcccgt gcgtcgtatg aggcagaaaa aagtattctt
    27001 cgccatattc tcgaaggtaa ggaggcggtc acgccgctga tggagagagt acccggcgaa
    27061 ctcatggaga cgttaacgtc gggacagcgt gccgccaccc gtatgatact ggaaacgtcc
    27121 gaccgtttca cggtggtaca gggttatgcc ggtgtgggta agaccacaca gttccgggcg
    27181 gtgatgtcag ccgtgaacat gctgccggcg agtgagcgtc cccgagttgt ggggctgggg
    27241 cccacgcacc gtgcggtcgg tgagatgcgc agcgccggcg tggatgcaca gacgctggcg
    27301 tcctttctgc atgacacgca gctgcagcag cgcagcggag aaacgccgga tttcagcaac
    27361 acgctgttcc tgctcgatga gagctcaatg gtgggcaata ccgacatggc acgggcatac
    27421 gccctgattg cggccggtgg cggtcgtgcc gtggccagtg gtgacacgga ccagctgcag
    27481 gccatcgcgc ccggtcagcc tttccgtctc cagcagacgc gcagtgctgc cgatgtggtc
    27541 atcatgaagg agattgtgcg tcagacgccg gaactgcggg aggcggtata cagcctgatt
    27601 aaccgggatg tggaaagggc actgtccggg cttgagagtg tgaaaccgtc tcaggttccc
    27661 cgtcaggagg gcgcatgggc accggagcac tccgtgacgg agttcagtca cagccaggaa
    27721 gcgaaactgg cagaagcgca gcagaaggcg atgctgaaag gtgagacttt tccggatgtc
    27781 cccatgacac tgtatgaagc cattgtccgc gactataccg gcaggacacc ggaagcacgg
    27841 gagcagacgc tgattgtcac gcacctgaat gaggaccggc gcgtactgaa cagcatgatt
    27901 catgatgcac gggaaaaggc cggtgagctg ggcaaagagc aggtcatggt gcctgtcctg
    27961 aacacagcga atatacgtga cggagaactg cgtcgtctct ccacctggga gaaaaacccg
    28021 gatgcccttg ccctggtgga taatgtgtat caccggattg ccggtatcag caaggatgac
    28081 gggctgataa ccctgcagga tgcggaaggt aacacgcggc tgatttcgcc ccgggaggcg
    28141 gtggctgaag gtgtcacact gtacacacca gacaaaatcc gggtgggaac cggcgaccgg
    28201 atgcgcttca cgaagagtga ccgggagcgc ggttatgtgg ccaacagcgt ctggacggtg
    28261 acagcggttt ccggtgacag tgtcacgctg tcagacggac agcagacccg ggtgattcgc
    28321 cccggccagg agcgggcaga gcaacatatt gacctggctt atgccatcac cgcccacggt
    28381 gcgcaggggg cgagtgaaac ctttgccatc gcgcttgaag gcacggaagg taaccggaaa
    28441 ctgatggccg gctttgagtc agcctacgtg gccctgtcgc gtatgaagca gcatgtgcag
    28501 gtgtacaccg ataaccgtca gggctggacg gatgccatta acaatgccgt acagaaagga
    28561 actgcccacg atgtgctgga gccgaaaccg gaccgggagg tcatgaatgc gcagcggctg
    28621 ttcagtacgg cacgggaact gcgggacgtg gcggcaggcc gtgccgttct ccgtcaggca
    28681 gggctggccg ggggagacag ccctgcacgg tttattgcac cgggacgtaa atatccgcag
    28741 ccgtatgtgg cactgccggc gtttgaccgt aacggcaagt cagctggtat ctggctgaac
    28801 ccactgacca cggatgacgg aaacgggctg cggggattca gtggtgaagg acgggtgaaa
    28861 ggcagcgggg atgcgcagtt cgtggccctg cagggcagcc gtaacggaga gagcctgctg
    28921 gctgataata tgcaggatgg cgtccggatt gcccgtgata atcctgacag cggtgtggtg
    28981 gtgagaattg ccggtgaagg tcgtccgtgg aatcccggtg ccatcaccgg tggccgcgtg
    29041 tggggggata tcccggataa cagcgtccag ccgggggccg gaaatggcga accggtcacg
    29101 gcagaggtac tggcacagcg gcaggctgaa gaggccatcc gccgtgaaac ggaacgccgc
    29161 gcagatgaaa ttgtcaggaa aatggcagag aacaaacctg acctgccgga cggcaaaaca
    29221 gagctggctg tcagggatat tgccggacag gagcgtgacc ggtctgccat atctgaacgg
    29281 gaaaccgcac tgccggagag tgtgctgcgt gaatcacaac gggagcggga agcggtccgg
    29341 gaggttgccc gggaaaatct gctgcaggag cgtctgcagc agatggagcg ggatatggtt
    29401 cgtgacctgc agaaagagaa aaccctgggc ggagactgat acaggaagac aaacactgat
    29461 gacaacggat aacacgaaca cgacacgtaa cgattcactg gctgcacgga ccgatacctg
    29521 gttgcagtcg tttctggtct ggtcacccgg acagcgggat atcatcaaaa cggtggcgct
    29581 ggtgctgatg gtactggacc acatcaatct gatattccag ctgaagcagg aatggatgtt
    29641 tctggccggg cgaggagcct ttccgctgtt tgccctggtg tgggggctga atctgtcccg
    29701 tcatgcgcat atccggcaac cggccattaa ccggctgtgg ggatggggaa ttattgccca
    29761 gttcgcatat tatctggccg gctttccctg gtatgagggg aatatcctgt ttgcctttgc
    29821 agtggcggca caggtgctga cgtggtgtga aacgcgcagt gggtggcgta cagcagcggc
    29881 cattcttctg atggccctgt gggggccttt gtccggcacc agttacggca ttgccgggct
    29941 gctgatgctg gcggtcagtt accgactgta ccgggcggaa gacagagcgg aacgtctggc
    30001 gctgcttgca tgcctgctgg ccgttattcc ggcacttaac cttgcctcca gtgatgcagc
    30061 ggcggtggcc ggtcttgtaa tgacggtgct gaccgttggt ctggtgtcgt gtgcaggaaa
    30121 atcattaccc cgtttctggc ccggggattt tttcccggta ttctacgcct gtcatctggc
    30181 tgtgctgggc gttctggcgt tgtgactggt atgatactga cgctgattct gattattaat
    30241 aaacaacata acacaggata tttatgaaaa ttacggatca caaactctct gaaggtattg
    30301 ccctgacatt tcgtgttcct gaaggaaata taaaacatcc tttgattatc ttatgccatg
    30361 ggttatgcgg tatcaggaat gttctgctcc cttgctttgc gaatgccttc acagaagcag
    30421 gattcgcaac gattactttt gattatcgag gattcggtga gagtgatgga gaacgagggc
    30481 gtctggttcc tgcaatgcag accgaagata ttatttcagt tatcaattgg gcagaaaagc
    30541 aggaatgtat tgataatcag cgtattgggt tatggggaac atcgctggga ggggggcatg
    30601 tcttcagtgc agctgcgcag gaccaacgcg taaaatgtat tgtcagccag ctggcttttg
    30661 ctgacggtga tgttctggtt accggagaga tgaatgaatc ggagagagcg tcttttctgt
    30721 ccacgctgaa taaaatggca gagaagaaga aaaacaccgg gaaagagatg tttgtggggg
    30781 ttacccgggt actgagtgat aatgaatcaa aggtattctt cgagaaggta aaaggtcagt
    30841 atcctgaaat ggatataaaa atacctttcc ttacggttat ggagacgttg caatataaac
    30901 cggcagagag cgcagccaaa gtacagtgcc cagttttgat tgttattgcc ggccaggata
    30961 gtgttaatcc accagaacag ggaaaagcct tatatgatgc tgttgcttct ggtactaagg
    31021 aattatacga ggaagctgac gcatgccatt atgatattta tgaaggcgcg ttttttgaac
    31081 gtgtggcggc agtacagacg caatggttta aaaaacatct ttaattgtcg gtattaatat
    31141 gtcagaggtc gttgaggtca gcttaatatc atagtgagta gtttcaacga cctggaggac
    31201 atattttcac aaaggtcagg gcaatcatga cagagcagaa acgaccggta ctgacactga
    31261 aacggaaaac ggaaggggaa acaccgaccc ggagccggaa aaccatcatc aatgtcacca
    31321 cgccaccaaa atggaaggtg aaaaagcaga aactggcgga gaaggctgcc cgggaagcag
    31381 agctgacagc aaaaaaagcg caggccagac aggcgctgtc catttatctg aacctgccct
    31441 cgctggatga ggccgtgaac accctgaaac cctggtggcc gggattattt gacggtgaca
    31501 caccccgact tctggcctgc ggtatccggg acgtgttact ggaagacgtg gcgcagcgga
    31561 atatcccgct ctcgcataaa aaactgcgca gggcgctgaa ggccatcacc cgttcagaaa
    31621 gctatctgtg tgccatgaaa gccggtgcct gccggtatga cacggaaggg tatgtgacgg
    31681 agcatatttc tcaggaggaa gaagtgtatg cggcagagcg tctggataaa atccgccgcc
    31741 agaaccggat aaaggcagaa cttcaggccg tgcttgatga acaataaaaa agcctccttc
    31801 acactaaagg aggcggaaaa ataaggcttc tctgaaacct tcttccatac ccggcagaag
    31861 aaggttataa aaatttataa cacattcacc ggcctggatt aaagggggaa ggaaaatgaa
    31921 cggaacaggg cgtgattcgc aaaactccgg ggtatggcgc taccggcagg tcggacgccg
    31981 ggctgtccgg ctggaagtca ggggggactg gccgggagcc gctgaggcat ggctacgggc
    32041 tgcgcagtct gctccccgga cagactggca gcagtttgcc cgaaaaaggg ctgaacactg
    32101 ccaccggcgc tgccggggaa gggtgtaacg aaaaagcagc cttcattact ccagggattc
    32161 agaaggctga ataatgcatg agatttgctt tttttaatat ccggtcggaa agtatgaccg
    32221 gacagttctg agtagcacag agttttttcc atattaagtc gtgattttat attttg
//
LOCUS       DAHHTG010000045        32207 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.045, whole genome shotgun sequence.
ACCESSION   DAHHTG010000045 DAHHTG010000000
VERSION     DAHHTG010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 32207)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32207)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32207
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.045"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            complement(27..1706)
                     /gene="bcsG"
                     /locus_tag="NBI74_002073"
                     /pseudo
     CDS             complement(27..1706)
                     /gene="bcsG"
                     /locus_tag="NBI74_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312445.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsG"
     gene            complement(1703..1894)
                     /gene="bcsF"
                     /locus_tag="NBI74_002074"
     CDS             complement(1703..1894)
                     /gene="bcsF"
                     /locus_tag="NBI74_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsF"
                     /protein_id="HCD1932416.1"
                     /translation="MMTISDIIEIIVVCALIFFPLGYLARHSLRRIRDTLRLFFAKPR
                     YVKPAGTLRRTEKARATKK"
     gene            complement(1891..3462)
                     /gene="bcsE"
                     /locus_tag="NBI74_002075"
     CDS             complement(1891..3462)
                     /gene="bcsE"
                     /locus_tag="NBI74_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsE"
                     /protein_id="HCD1932417.1"
                     /translation="MRDIVDPVFSIGISSLWDELRHMPAGGVWWFNVDRHEDAISLAN
                     QTIASQAETAHVAVISMDSDPAKIFQLDDSQGPEKITLFSMLNHEKGLYYLARDLQCS
                     IDPHNYLFILVCANNAWQNIPAERLRSWLDKMNKWSRLNHCSLLVINSGNNNDKQFSL
                     LLEEYRSLFGLASLRFQGDQHLLDIAFWCNEKGVSARQQLSVQQQNGCWTLVQHQEAE
                     IQPRSDEKRILSNVAVLEGAPPLSEHWQLFNNNEVLFNEARTAQAATVVFSLQQNAQI
                     EPLARSIHTLRRQRGSAMKILVRENTASLRATDERLLLACGANMVIPWNAPLSRCLTM
                     IESVQGQKFSRYVPEDITTLLSMTQPLKLRGFQKWDVFCNAVNNMMNNPLLPAHGKGV
                     LVALRPVPGIRVEQALTLCRPNRTGDIMTIGGNRLVLFLSFCRINDLDTALNHIFPLP
                     TGDIFSNRMVWFEDDQISAELVQMRLLAPEQWGMPLPLAQSSKPVINAEHDGRHWRRI
                     PEPMRLLDDAVERSS"
     gene            3735..3923
                     /locus_tag="NBI74_002076"
     CDS             3735..3923
                     /locus_tag="NBI74_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhjR family protein"
                     /protein_id="HCD1932418.1"
                     /translation="MNNNEPDTLPDPAIGYIFQNDILALKQAFSLPDIDYADISQREQ
                     LAAALKRWPLLAEFAQQK"
     gene            3935..4687
                     /gene="bcsQ"
                     /locus_tag="NBI74_002077"
     CDS             3935..4687
                     /gene="bcsQ"
                     /locus_tag="NBI74_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsQ"
                     /protein_id="HCD1932419.1"
                     /translation="MAVLGLQGVRGGVGTTTITAALAWSLQMLGENVLVVDASPDNLL
                     RLSFNVDFTHRQGWARAMLDGQDWRDAGLRYTSQLDLLPFGQLSIEEQENPQHWQTRL
                     SDICSGLQQLKASGRYQWILIDLPRDASQITHQLLSLCDHSLAIVNVDANCHIRLHQQ
                     ALPDGAHILINDFRIGSQVQDDIYQLWLQSQRRLLPMLIHRDEAMAECLAAKQPVGEY
                     RSDALAAEEILTLANWCLLNYSGLKTPVGSAS"
     gene            4684..7302
                     /gene="bcsA"
                     /locus_tag="NBI74_002078"
     CDS             4684..7302
                     /gene="bcsA"
                     /locus_tag="NBI74_002078"
                     /EC_number="2.4.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417990.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-forming cellulose synthase catalytic
                     subunit"
                     /protein_id="HCD1932420.1"
                     /translation="MSILTRWLLIPPVNARLIGRYRDYRRHGASAFSATLGCFWMILA
                     WIFIPLEHPRWQRIRAEHKNLYPHINASRPRPLDPVRYLIQTCWLLIGASRKETPKPR
                     RRAFSGLQNIRGRYHQWMNELPERVSHKTQHLDEKKELGHLSAGTRRLILGIIVTFSL
                     ILALICVTQPFNPLAQFIFLMLLWGVALIVRRMPGRFSALMLIVLSLTVSCRYIWWRY
                     TSTLNWDDPVSLVCGLILLFAETYAWIVLVLGYFQVVWPLNRQPVPLPKDMSLWPSVD
                     IFVPTYNEDLNVVKNTIYASLGIDWPKDKLNIWILDDGGREEFRQFAQNVGVKYIART
                     THEHAKAGNINNALKYAKGEFVSIFDCDHVPTRSFLQMTMGWFLKEKQLAMMQTPHHF
                     FSPDPFERNLGRFRKTPNEGTLFYGLVQDGNDMWDATFFCGSCAVIRRKPLDEIGGIA
                     VETVTEDAHTSLRLHRRGYTSAYMRIPQAAGLATESLSAHIGQRIRWARGMVQIFRLD
                     NPLTGKGLKFAQRLCYVNAMFHFLSGIPRLIFLTAPLAFLLFHAYIIYAPALMIALFV
                     LPHMIHASLTNSKIQGKYRHSFWSEIYETVLAWYIAPPTLVALINPHKGKFNVTAKGG
                     LVEEEYVDWVISRPYIFLVLLNLVGVAVGIWRYIYGPPTEMLTVVVSMVWVFYNLIIL
                     GGAVAVSVESKQVRRSHRVEMTMPAAIAREDGHLFSCTVQDFSDGGLGIKINGQAQIL
                     EGQKVNLLLKRGQQEYVFPAQVARVMGNEVGLKLMPLTTQQHIDFVQCTFARADTWAL
                     WQDSYPEDKPLESLLDILKLGFRGYRHLAEFAPSSVKGIFRVLTSLVSWVVSFIPRRP
                     ERSETAQPSDQALAQQ"
     gene            7313..9652
                     /gene="bcsB"
                     /locus_tag="NBI74_002079"
     CDS             7313..9652
                     /gene="bcsB"
                     /locus_tag="NBI74_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis cyclic di-GMP-binding
                     regulatory protein BcsB"
                     /protein_id="HCD1932421.1"
                     /translation="MKRKIFWICAVALGMSAFPSFMTQATPATQPLINAEPAVAAQAE
                     QNPQVGQVMPGVQGADAPVVAQNGPSRDVKLTFAQIAPPPGSMVLRGINPNGSIEFGM
                     RSDEVVTKAMLNLEYTPSPSLLPVQSQLKVYLNDELMGVLPVTKEQLGKKTLAQMPIN
                     PLFITDFNRVRLEFVGHYQDVCENPASTTLWLDVGRSSGLDLTYQTLNVKNDLSHFPV
                     PFFDPRDNRTNTLPMVFAGAPDVELQQASAIVASWFGSRSGWRGQNFPVLYNQLPDRN
                     AIVFATNDKRPDFLRDHPAVKAPVIEMINHPQNPYVKLLVVFGRDDKDLLQAAKGIAQ
                     GNILFRGESVVVNEVKPLLPRKPYDAPNWVRTDRPVTFGELKTYEEQLQSSGLEPAAI
                     NVSLNLPPDLYLMRSTGIDMDINYRYTMPPVKDSSRMDISLNNQFLQSFNLSSKQEAN
                     RLLLRIPVLQGLLDGKTDVSIPALKLGATNQLRFDFEYMNPMPGGSVDNCITFQPVQN
                     HVVIGDDSTIDFSKYYHFIPMPDLRAFANAGFPFSRMADLSQTITVMPKTPNEAQMET
                     LLNTVGFIGAQTGFPAINLTVTDDGSTIQGKDADIMIVGGIPDKLKDDKQIDLLVQAT
                     ESWVKTPMRQTPFPGIVPDESDRAAETQSTLTSSGAMAAVIGFQSPYNDQRSVIALLA
                     DSPRGYEMLNDAVNDSGKRATMFGSVAVIRESGINSLRVGDVYYVGHLPWFERLWYAL
                     ANHPILLAVLAAISVILLAWVLWRLLRIISRRRLNPDNE"
     gene            9659..10765
                     /gene="bcsZ"
                     /locus_tag="NBI74_002080"
     CDS             9659..10765
                     /gene="bcsZ"
                     /locus_tag="NBI74_002080"
                     /EC_number="3.2.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase"
                     /protein_id="HCD1932422.1"
                     /translation="MNVLRSGLVTMLLLAAFSVQAACTWPAWEQFKKDYISQEGRVID
                     PSDARKITTSEGQSYGMFFALAANDRAAFDNLLDWTQNNLAQGSLKENLPAWLWGKKE
                     NSKWEVLDSNSASDGDVWMAWSLLEAGRLWKEQRYTDIGSALLKRIAREEVVTVPGLG
                     SMLLPGKVGFAEDNSWRFNPSYLPPTLAQYFTRFGAPWTTLRETNQRLLLETAPKGFS
                     PDWVRYEKDKGWQLKAEKTLISSYDAIRVYMWVGMMPDSDPQKARMLNRFKPMATFTE
                     KNGYPPEKVDVATGKAQGKGPVGFSAAMLPFLQNRDAQAVQRQRVADNFPGSDAYYNY
                     VLTLFGQGWDQHRFRFSTKGELLPDWGQECANSH"
     gene            10747..14220
                     /gene="bcsC"
                     /locus_tag="NBI74_002081"
     CDS             10747..14220
                     /gene="bcsC"
                     /locus_tag="NBI74_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026226.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsC"
                     /protein_id="HCD1932423.1"
                     /translation="MRKFTLNIFTLSLGLAVMPMVEAAPTAQQQLLEQVRLGEATHRE
                     DLVQQSLYRLELIDPNNPDVIAARFRSLLRQGDIDGAQKQLDRLSQLAPSSNAYKSSR
                     TTMLLSTPDGRQALQQARLQATTGHAEEAVASYNKLFNGAPPEGDIAVEYWSTVAKIP
                     ARRGEAINQLKRINADTPGNTGLQNNLALLLFSSDRRDEGFAVLEQMAKSNAGREGAS
                     KIWYGQIKDMPVSDASVQALKKYLSIFSDGDSVAAAQSQLAEQQKQLADPAFRARAQG
                     LAAVDSGMAGKAIPELQQAVRANPKDSEALGALGQAYSQKGDRANAVANLEKALALDP
                     HNSNNDKWNSLLKVNRYWLAIQQGDAALKANNPDRAERLFQQARNVDNTDSYAVLGLG
                     DVAMARKDYPAAERYYQQTLRMDSGNTNAVRGLANIYRQQSPEKAEAYIASLSASQRR
                     SIDDIERSLQNDRLAQQAEALENQGKWAQAAALQRQRLALDPGSVWITYRLSQDLWQA
                     GQRSQADTLMRNLAQQKPNDPEQVYAYGLYLSGHDQDRAALAHINSLPRAQWNSNIQE
                     LVNRLQNDQVLETANRLRENGKEAEAEALLRQQPPSSRIDLTLADWAQQRRDYTAARA
                     AYQNVLTREPTNADAILGLTEVDIAAGDKAAARSQLAKLPATDNASLNTQRRVALAQA
                     QLGDTAAAQQTFNKLIPQAKSQPPSMESAMVLRDGAKFEAQAGDPMQALETYKDAMVA
                     SGVTTTRPQDNDTFTRLTRNDEKDDWLKRGVRSDAADLYRQQDLNVTLEHDYWGSSGT
                     GGYSDLKAHTTMLQVDAPYSDGRMFFRSDFVNMNVGSFSTNADGKWDDNWGTCTLQDC
                     SGNRSQSDSGASVAVGWRNDVWSWDIGTTPMGFNVVDVVGGISYSDDIGPLGYTVNAH
                     RRPISSSLLAFGGQKDSPSNTGKKWGGVRADGVGLSLSYDKGEANGVWASLSGDQLTG
                     KNVEDNWRVRWMTGYYYKVINQNNRRVTIGLNNMIWHYDKDLSGYSLGQGGYYSPQEY
                     LSFAIPVMWRERTENWSWELGASGSWSHSRTKTMPRYPLMNLIPTDWQEEAARQSNDG
                     GSSQGFGYTARALLERRVTSNWFVGTAIDIQQAKDYAPSHFLLYVRYSAAGWQGDMDL
                     PPQPLIPYADW"
     gene            14302..16290
                     /gene="hmsP"
                     /locus_tag="NBI74_002082"
     CDS             14302..16290
                     /gene="hmsP"
                     /locus_tag="NBI74_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator HmsP"
                     /protein_id="HCD1932424.1"
                     /translation="MRVSRSLTIKQMAMVAAVVLVFVFIFCTVLLFHLVQQNRYNTAT
                     QLESIARSVREPLSSAILKGDIPEAEAILASIKPAGVVSRADVVLPNQFQALRKSFIP
                     ERPVPVMVTRLFELPVQISLGVYSLERPANPQPIAYLVLQADSFRMYKFVMSTLSTLV
                     TIYLLLSLILTVAISWCINRLILHPLRNIARELNAIPPQDLVGHQLALPRLHQDDEIG
                     MLVRSYNLNQQLLQRHYEEQNENAMRFPVSDLPNKALLMEMLEQVVARKQTTALMIIT
                     CETLRDTAGVLKEAQREILLLTLVEKLKSVLSPRMILAQISGYDFAVIANGVQEPWHA
                     ITLGQQVLTIMSERLPIERIQLRPHCSIGVAMFYGDLTAEQLYSRAISAAFTARHKGK
                     NQIQFFDPQQMEAAQKRLTEESDILNALENHQFAIWLQPQVEMTSGKLVSAEVLLRIQ
                     QPDGSWDLPDGLIDRIECCGLMVTVGHWVLEESCRLLAAWQERGIMLPLSVNLSALQL
                     MHPNMVADMLELLTRYRIQPGTLILEVTESRRIDDPHAAVAILRPLRNAGVRVALDDF
                     GMGYAGLRQLQHMKSLPIDVLKIDKMFVEGLPEDSSMIAAIIMLAQSLNLQMIAEGVE
                     TEAQRDWLAKAGVGIAQGFLFARPLPIEIFEESYLEEK"
     gene            16473..17759
                     /gene="dctA"
                     /locus_tag="NBI74_002083"
     CDS             16473..17759
                     /gene="dctA"
                     /locus_tag="NBI74_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate transporter DctC"
                     /protein_id="HCD1932425.1"
                     /translation="MKTSLFKSLYFQVLTAIAIGILLGHFYPEIGEQMKPLGDGFVKL
                     IKMIIAPVIFCTVVTGIAGMESMKAVGRTGAVALLYFEIVSTIALIIGLIIVNVVQPG
                     AGMNVDPATLDAKAVAVYADQAKDQGIVAFIMDVIPASVIGAFASGNILQVLLFAVLF
                     GFALHRLGSKGQLIFNVIESFSQVIFGIINMIMRLAPIGAFGAMAFTIGKYGVGTLVQ
                     LGQLIICFYITCILFVVLVLGSIAKATGFSIFKFIRYIREELLIVLGTSSSESALPRM
                     LDKMEKLGCRKSVVGLVIPTGYSFNLDGTSIYLTMAAVFIAQATNSQMDIVHQITLLI
                     VLLLSSKGAAGVTGSGFIVLAATLSAVGHLPVAGLALILGIDRFMSEARALTNLVGNG
                     VATIVVAKWVKELDHKKLDDVLNNRAPDGKTHELSS"
     gene            17979..19475
                     /locus_tag="NBI74_002084"
     CDS             17979..19475
                     /locus_tag="NBI74_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HCD1932426.1"
                     /translation="MQGTKIRLLAGGLLMMATAGYVQADALQPDPAWQQGTLSNGLQW
                     QVLTTPQRPSDRVEIRLLVNTGSLAESTQQSGYSHAIPRIALTQSGGLDAAQARSLWQ
                     QGIDPKRPMPPVIVSYDTTLFNLSLPNNRNDLLKEALSYLANATGKLTITPETINHAL
                     QSQDMVATWPADTKEGWWRYRLKGSTLLGHDPADPLKQPVEAEKIKDFYQKWYTPDAM
                     TLLVVGNVDARSVVDQINKTFGELKGKRETPAPVPTLSPLRAEAVSIMTDAVRQDRLS
                     IMWDTPWQPIRESAALLRYWRADLAREALFWHVQQALSASNSKDIGLGFDCRVLYLRA
                     QCAINIESPNDKLNSNLNLVARELAKVRDKGLPEEEFNALVAQKKLELQKLFAAYARA
                     DTDILMGQRMRSLQNQVVDIAPEQYQKLRQDFLNSLTVEMLNQDLRQQLSNDMALILL
                     QPKGEPEFNMKALQAAWDQIMAPSTAAAATSVATDDVHPEVTDIPPAQ"
     gene            complement(19571..20500)
                     /gene="kdgK"
                     /locus_tag="NBI74_002085"
     CDS             complement(19571..20500)
                     /gene="kdgK"
                     /locus_tag="NBI74_002085"
                     /EC_number="2.7.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygluconokinase"
                     /protein_id="HCD1932427.1"
                     /translation="MSKKIAVIGECMIELSEKGADVKRGFGGDTLNTSVYIARQVDPA
                     ALTVHYVTALGTDSFSQQMLDAWRGENVDTSLTQRMENRLPGLYYIETDSTGERTFYY
                     WRNEAAAKFWLESEQSATICEELANFDYLYLSGISLAILSPTSREKLLSLLRECRANG
                     GKVIFDNNYRPRLWASKEETQQVYQQMLECTDIAFLTLDDEDALWGQQPVEDVIARTH
                     NAGVKEVVVKRGADSCLVSIAGEGLVDVPAVKLPKEKVIDTTAAGDSFSAGYLAVRLT
                     GGSAEDAAKRGHLTASTVIQYRGAIIPREAMPA"
     gene            20732..21499
                     /gene="pdeH"
                     /locus_tag="NBI74_002086"
     CDS             20732..21499
                     /gene="pdeH"
                     /locus_tag="NBI74_002086"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709303.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase"
                     /protein_id="HCD1932428.1"
                     /translation="MIRQVIQRISNPEASIESLQERRFWLQCERAYTWQPIYQTCGRL
                     MAVELLTVVTHPLNPSQRLPPDRYFTEITVSHRMEVVKEQIDLLAQKADFFVEHGLLA
                     SVNIDGPTLIALRQQPKILRQIEELPWLRFELVEHIRLPKDSTFASMCEFGPLWLDDF
                     GTGMANFSALSEVRYDYIKIARELFVMLRQSPEGRTLFSQLLHLMNRYCRGVIVEGVE
                     TPEEWRDVQNSPAFAAQGWFLSRPAPIETLNTAVLAL"
     gene            21569..23629
                     /locus_tag="NBI74_002087"
     CDS             21569..23629
                     /locus_tag="NBI74_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HCD1932429.1"
                     /translation="MSKAGKITAAISGAFLLLIVVAIILIATFDWNRLKPTINQKVSA
                     ELNRPFAIRGDLGVVWERQKQETGWRSWVPWPHVHAEDIILGNPPDIPEVTMVHLPRV
                     EATLAPLALLTKTVWLPWIKLEKPDARLIRLSEKNNNWTFNLANDDNKDANAKPSAWS
                     FRLDNILFDQGRIAIDDKVSKADLEIFVDPLGKPLSFSEVTGSKGKADKEKVGDYVFG
                     LKAQGRYNGEPLTGTGKIGGMLALRGEGTPFPVQADFRSGNTRVAFDGVVNDPMKMGG
                     VDLRLKFSGDSLGDLYELTGVLLPDTPPFETDGRLVAKIDTEKSSVFDYRGFNGRIGD
                     SDIHGSLIYTTGKPRPKLEGDVESRQLRLADLGPLIGVDSGKGAEKSKRSEQKKGEKS
                     VQPAGKVLPYDRFETDKWDVMDADVRFKGRRIEHGSSLPISDLSTHIILKNADLRLQP
                     LKFGIAGGSIAANIHLEGDKKPMQGRADIQARRLKLKELMPDVELMQKTLGEMNGDAE
                     LRGSGNSVAALLGNSNGNLKLLMNDGLVSRNLMEIVGLNVGNYIVGAIFGDDEVRVNC
                     AAANLDIANGVARPQIFAFDTENALINVTGTASFASEQLDLTIDPESKGIRIITLRSP
                     LYVRGTFKNPQAGVKAGPLIARGAVAAALATLVTPAAALLALISPSEGEANQCRTILS
                     QMKK"
     gene            complement(23808..25130)
                     /locus_tag="NBI74_002088"
     CDS             complement(23808..25130)
                     /locus_tag="NBI74_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MHS family MFS transporter"
                     /protein_id="HCD1932430.1"
                     /translation="MQATATTLDHEQEYTPINSRNKVLVASLIGTAIEFFDFYIYATA
                     AVIVFPHIFFPQGDPTAATLQSLATFAIAFVARPIGSAVFGHFGDRVGRKATLVASLL
                     TMGISTVVIGLLPGYATIGIFAPLLLALARFGQGLGLGGEWGGAALLATENAPPRKRA
                     LYGSFPQLGAPIGFFFANGTFLLLSWLLTDEQFMSWGWRVPFIFSAVLVIIGLYVRVS
                     LHESPVFEKVAKAKKQVKIPLGTLLTKHVRVTVLGTFIMLATYTLFYIMTVYSMTFST
                     AAAPVGLGLPRNEVLWMLMMAVIGFGVMVPVAGLLADAFGRRKSMVIITTLIILFALF
                     AFTPLLGSGNPILVFAFLLLGLSLMGLTFGPMGALLPELFPTEVRYTGASFSYNVSSI
                     LGASVAPYIAAWLQTNYGLGAVGLYLAAMAGLTLIALLLTHETRHQSL"
     gene            complement(25523..26536)
                     /gene="yhjD"
                     /locus_tag="NBI74_002089"
     CDS             complement(25523..26536)
                     /gene="yhjD"
                     /locus_tag="NBI74_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YhjD"
                     /protein_id="HCD1932431.1"
                     /translation="MTQENEIKRPTQDLEHEPIKQLDNSEKGGKVSQALETVTTTAEK
                     VQRQPVIAHLIRATERFNDRLGNQFGAAITYFSFLSMIPILMVSFAAGGFVLASHPML
                     LQDIFDKILQNISDPTLAATLKNTINTAVQQRTTVGLVGLAVALYSGINWMGNLREAI
                     RAQSRDVWERSPQDQEKFWVKYLRDFISLIGLLIALIVTLSITSVAGSAQQMIISALH
                     LNSIEWLKPTWRLIGVAISIFANYLLFFWIFWRLPRHRPRKKALIRGTFLAAIGFEVI
                     KIVMTYTLPSLMKSPSGAAFGSVLGLMAFFYFFARLTLFCAAWIATAEYKDDPRMPGK
                     TQP"
     gene            complement(26584..27483)
                     /locus_tag="NBI74_002090"
     CDS             complement(26584..27483)
                     /locus_tag="NBI74_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417978.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCD1932432.1"
                     /translation="MDKIHAMQLFIKVAELESFSRAADFFALPKGSVSRQIQALEHQL
                     GTQLLQRTTRRVKLTPEGMTYYQRAKDVLSNLSELDGLFQQDATSISGKLRIDIPSGI
                     AKSLLLPRLSEFLYLHPGIELELSSHDRPVDILHDGFDCVIRTGALPEDGVIARPLGK
                     LTMVNCASPHYLTRFGYPQSPDDLTSHAIVRYTPHLGVHPLGFEVASVNGVQWFKSGS
                     MLTVNSSENYLAAGLAGLGIIQIPRIAVREALRAGRLIEVLPGYRAEPLSLSLVYPQR
                     RELSRRVNLFMQWLAGVMKEYLD"
     gene            28003..28605
                     /locus_tag="NBI74_002091"
     CDS             28003..28605
                     /locus_tag="NBI74_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCD1932433.1"
                     /translation="MQIVMFDRQSIFIHGMKISLQHRIPGVSIQGASQADELWQKLES
                     YPEALVMLDGDQDGEFCYWLLQKTVVQFPEVKVLITATDCNKRWLQEVIHFNVLAIVP
                     RDSTVETFALAVNSAAMGMMFLPGDWRTTPEKDIKDLKSLSARQREILTMLAAGESNK
                     EIGRALNISTGTVKAHLESLYRRLEVKNRTQAAMMLNISS"
     gene            complement(28656..30305)
                     /locus_tag="NBI74_002092"
     CDS             complement(28656..30305)
                     /locus_tag="NBI74_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
                     /protein_id="HCD1932434.1"
                     /translation="MLNQKIQNPNPDELMIEVDLCYELDPYELKLDEMIEAEPEPEMI
                     EGLPASDALTPADRYLELFEHVQSAKIFPDSKTFPDCAPKMDPLDILIRYRKVRRHRD
                     FDLRKFVENHFWLPEVYSSEYVSDPQNSLKEHIDQLWPVLTREPQDHIPWSSLLALPQ
                     SYIVPGGRFSETYYWDSYFTMLGLAESGREDLLKCMADNFAWMIENYGHIPNGNRTYY
                     LSRSQPPVFALMVELFEEDGVRGARRYLDHLKMEYAFWMDGAESLIPNQAYRHVVRMP
                     DGSLLNRYWDDRDTPRDESWLEDVETAKHSGRPPNEVYRDLRAGAASGWDYSSRWLRD
                     TGRLASIRTTQFIPIDLNAFLFKLESAIANISALKGEKETEALFRQKASARRDAVNRY
                     LWDDENGIYRDYDWRREQLALFSAAAIVPLYVGMANHEQADRLANAVRSRLLTPGGIL
                     ASEYETGEQWDKPNGWAPLQWMAIQGFKMYGDDLLGDEIARNWLKTVNQFYLEQHKLI
                     EKYHIADGVPREGGGGEYPLQDGFGWTNGVVRRLIGLYGEP"
     gene            30710..32107
                     /gene="ccp"
                     /locus_tag="NBI74_002093"
     CDS             30710..32107
                     /gene="ccp"
                     /locus_tag="NBI74_002093"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HCD1932435.1"
                     /translation="MKMVSRITAIGLAGVAICYLGLSGYVWYHDNKRSKQADVQASAV
                     SENNKVLGFLREKGCDYCHTPSAELPAYYYIPGAKQLMDYDIKLGYKSFNLEAVRAAL
                     LANKPVSQSDLNKIEWVMQYETMPPTRYTALHWAGKVSDEERAEILAWIAKQRAEYYA
                     SNDTAPEHRNEPVQPIPQKLPTDAQKVALGFALYHDPRLSADSTISCAHCHALNAGGV
                     DGRKTSIGVGGAVGPINAPTVFNSVFNVEQFWDGRAATLQDQAGGPPLNPIEMASKSW
                     DEIIAKLEKDPQLKAQFLEVYPQGFSGENITDAIAEFEKTLITPDSPFDKWLRGDENA
                     LTAQQKKGYQLFKDNKCATCHGGIILGGRSFEPLGLKKDFNFGEITAADIGRMNVTKE
                     ERDKLRQKVPGLRNVALTAPYFHRGDVPTLDGAVKLMLRYQVGKELPQEDVDDIVAFL
                     HSLNGVYMPYMQDKQ"
BASE COUNT         7730 a         8051 c         8798 g         7628 t
ORIGIN      
        1 gggaaactct gcggcctttt tcgttcttat tgcgggtaag gcacccagtc gccgccgttc
       61 aggcgaacgt acggtttatt ctggtattga ataactactg catttgagtt ctcggagacc
      121 ggtgctgttt gtggtaaccc actggtgagt tttttccagt caacattgtc ttcggtgaaa
      181 atcttgccat caagaacgcg aaccaccaga tcggagatag ccaggaagct gctcggttgg
      241 tcgatgacaa tcggagcccc ctgatgtggt gccttcatgc cgaagaattt caccccaacg
      301 gggacgtctg tgatagacgg gctagggata tcacgtagac cagatacctg cattctgtcg
      361 cccttcagcg cgccgccgtg ttccggcacc actaccacca tcactttacg acccgatttc
      421 tccagttcag taaagaaggc gtccagttca tcaaagaatt tttgcgcccg cgctttgtaa
      481 tccgctgttt tgctgacgcc cggataatgg ttgccgtcat gcagtggaag cgtgttgtag
      541 aacgtggcac tacggctgtt tttatctttt tcggtaacgt ccagccaacg gttaagcacg
      601 gcggtatcgt cataaaccgg cgaaccatca aagcccagca aaataaccgg cagatttgtt
      661 tgatccatca attcgctctg catgccgcca ttttcgcgaa cttctttcaa aaaaccgccg
      721 aactggccgt tatgtcccat catcaggtgc tgggtaaagc ccagtttcga caggttatca
      781 aacagatagc agtcgttatt tgccggttga tacagattag tgtgcgaagt ctgcccgcag
      841 ctggcgcgca gtaaacggat cgccgccggg ccactgtagg aggtggcgga gttaaagttc
      901 ttgaactcaa tatcgaaatg cgaccacagt ggatgcgaca tcaatccggc ggcttcaata
      961 tccgaccagg aaagcgaaca gatattaatc accagtagtt caaatggctg agcatcagcg
     1021 ggcagcgaag acgggaagat cgatttacgt ttcgcctccg cgttatagaa attattcagc
     1081 caggcgttaa ggtttgctgt tgttggcggt gcagtttgtg cgggcatatc acccactatc
     1141 ggtgcgccac ccgtcgccgc aacggttgcc gctgcgttac cacccgtcgt tgttacagtg
     1201 gtcgttggtt gaccggctgg ccacaaggag aaacttggtc ccgccagggt aagtacgttc
     1261 agccatagca gaatggcaac cacaaaaacg gtaatgcgaa tccattgtga caggaataac
     1321 caggccacga ataaaacaaa aatggcccca atcatctgcc agttaataaa gcgtgtgaca
     1381 agatcgatta aataatcggt actgaacccc gccacctgcg aaccctggct cattatgctt
     1441 tccggaccag gcaactaggt gtcatgccag aacaaagcaa agccgatcgg cagggcaatc
     1501 cagtggcgca agcgatgcag gctatagtgc ggaatgggca tcagtagaaa cgcggcaaac
     1561 accaaattga ggagcggatg gaagttaaga tatcccgccc acaacaggcc gaacttaacc
     1621 agaaaataga agttccagcc ggaaaggccg cgccagtatt gccagaggga agaaggcatg
     1681 gcggtatttt gcgtaaattg agtcattttt tggttgccct ggctttttcc gtgcggcgta
     1741 acgtcccggc cggtttaaca taacgaggtt tagcaaagaa caaacgtaag gtgtcgcgaa
     1801 tgcgtcgcaa agagtgccgc gccagatagc ccagcgggaa aaatatcagt gcgcaaacga
     1861 caataatttc aatgatatcg ctgatggtca tcatgatgag cgctccacag catcatctaa
     1921 cagtcgcatt ggttctggta ttcgtcgcca gtggcgacca tcatgctcgg cattgatgac
     1981 cggtttagaa ctttgcgcta aaggcagcgg catgccccat tgttctgggg caagcaggcg
     2041 catctgcacc agctcggcac tgatttgatc atcttcaaac cagaccattc ggtttgagaa
     2101 aatgtcgcca gtaggcaatg ggaaaatatg attcaacgcg gtatccagat cgttaatccg
     2161 acagaatgag agaaacagca ccagccgatt accgccaatg gtcatgatat cgccggtacg
     2221 gttagggcga catagcgtca gggcttgctc aacgcggata cccggtaccg gacgtagggc
     2281 aaccagaacg cctttaccgt gggcaggtaa tagagggtta ttcatcatgt tgttgacggc
     2341 attacagaac acatcccact tctggaaacc acgcagtttg agcggctggg tcattgacag
     2401 caaggtagtg atatcttccg gcacatagcg actaaacttc tgcccttgca cgctttcgat
     2461 catcgtcaga cagcgggaga gtggcgcatt ccacgggata accatatttg caccgcaggc
     2521 caataataaa cgttcatcgg tggcgcgcag gctggcggta ttttcccgta cgaggatttt
     2581 catcgcacta ccgcgctggc gacgcagagt atgaatgctg cgggccagtg gctcgatttg
     2641 cgcattttgc tgtaaagaaa agaccaccgt cgccgcctga gcggtacggg cttcattgaa
     2701 caggacttcg ttattgttga acagttgcca gtgttccgat agcggcggcg caccttccag
     2761 tacagcaaca ttactcagaa tgcgtttttc gtcgctgcgt ggttggattt ccgcctcttg
     2821 gtgttgaact aatgtccagc aaccattttg ttgctgaacg ctaagttgct gacgggcgct
     2881 gacccctttt tcgttgcacc agaaagcaat atccagcaaa tgttggtcgc cctgaaaacg
     2941 caaactggca agaccaaaaa gtgaccgata ttcctcaagt aacaatgaaa attgtttatc
     3001 gttattattt ccggaattaa ttaccaaaag cgaacaatgg tttagccggc tccatttatt
     3061 cattttatcc aaccatgagc gaagccgctc ggcaggaatg ttttgccatg cattatttgc
     3121 gcaaacaaga ataaaaaggt aattatgggg atcaatagaa cactgcaaat cacgggccaa
     3181 atagtataga cctttttcat gatttagcat cgaaaataat gttatttttt ccggcccttg
     3241 agaatcatct aattgaaaga ttttcgctgg atcgctgtcc atgctaatga ccgcgacgtg
     3301 tgcggtttca gcctgggatg caattgtttg attcgccaga ctgatagcat cttcatggcg
     3361 atcgacgtta aaccaccaga cgccgcctgc tggcatatgt cgcagctcat cccataatga
     3421 tgagataccg atagagaata cagggtccac aatgtccctc atgtcaacat tttgtctgat
     3481 tatcagttta ctagcgaaag ctaagaaata aacctaccat tgaaattaaa acttatcaga
     3541 tttagcatgt gaatcaattt agcaggtcac aagacatgtg attttagtct tttcgttttt
     3601 aactttttct gttgcataac ggaaagtcaa aaagtgagca cattcccgtc tcgccgtaaa
     3661 caataaaccg acgaaatgct cacagttaga attgtctcat gaacggtacg gttatttcat
     3721 agggatcaag aaaaatgaat aacaatgaac cagatactct gcctgatccc gcgataggct
     3781 atatcttcca gaatgatatt ttggcgttaa agcaggcatt ttcactgcct gatattgatt
     3841 atgccgatat ttcccaacgc gaacagttgg ccgcggcatt aaaacgctgg ccgttgctgg
     3901 cagagtttgc gcaacaaaag taggggattg gtgaatggcc gtactgggat tgcagggggt
     3961 tcggggaggc gtggggacaa caaccatcac cgccgcatta gcctggtcat tacaaatgtt
     4021 gggagaaaat gtcctggtgg tcgatgccag cccggacaac ttgttgcgcc tgtcatttaa
     4081 cgttgatttt acccaccgtc agggttgggc cagagctatg ctggatggcc aggactggcg
     4141 tgacgccggg ttgcgctata cctcgcagct cgatttgctg ccttttggtc agttatcgat
     4201 tgaagaacaa gaaaatccac agcactggca aacccggctg agcgatattt gctccggctt
     4261 acagcaacta aaagccagcg ggcgttacca gtggatttta attgacttac cgcgtgatgc
     4321 ctcgcagata acccaccagc tgctgagttt gtgcgatcac tcgctggcaa tcgtcaatgt
     4381 ggatgccaac tgccatatcc gactgcatca gcaagcgctg ccggatggtg cacatatttt
     4441 gattaatgac ttccgtattg gcagccaggt tcaggacgat atttaccagc tttggttgca
     4501 aagccagcgc cgattgctgc cgatgctcat tcatcgtgat gaagcgatgg ctgaatgtct
     4561 ggcagctaag caaccagtcg gtgaatatcg cagtgatgcg ctggcggctg aagagatact
     4621 gacgctggcg aactggtgcc tgttgaatta ttccgggctg aaaacgccag tcgggagtgc
     4681 atcatgagta tcctgacccg gtggttgctt atcccgccgg tcaacgcacg gcttattggg
     4741 cgttatcgcg attatcgtcg tcacggcgcg tcggctttca gcgcgacgct cggctgtttc
     4801 tggatgatcc tggcctggat ttttattccg ctggagcacc cgcgctggca gcgtattcgc
     4861 gcagaacata aaaacctgta tccgcatatc aacgcctcgc gtccgcgtcc gctggacccg
     4921 gtccgttatc ttattcaaac atgctggtta ctgatcggtg catcgcgcaa agaaacgccg
     4981 aaaccgcgca ggcgggcatt ttcaggtcta caaaatattc gtggacgtta ccatcaatgg
     5041 atgaatgagc tgcctgagcg cgttagccat aaaacacagc atcttgatga gaaaaaagag
     5101 ctcggtcatt tgagtgccgg gacgcggcgg ttgatcctcg gtatcatcgt caccttctcg
     5161 ctgattctgg cgttaatctg cgttactcag ccgtttaacc cgctagcgca gtttatcttc
     5221 ctgatgctgc tgtggggggt agcgctgatc gtacggcgga tgccggggcg cttctcggcg
     5281 ctgatgctga ttgtgctgtc gctgaccgtt tcttgccgtt atatctggtg gcgttacacc
     5341 tcgacgttaa actgggacga tccggtcagc ctggtgtgcg ggcttattct gctcttcgct
     5401 gaaacgtacg cgtggattgt gctggtgctc ggctatttcc aggtcgtatg gccgctgaat
     5461 cgtcagccgg tgccattgcc gaaagatatg tcgctgtggc cgtcggtgga tatctttgtc
     5521 ccgacttaca acgaagatct caacgtggtg aaaaatacca tttacgcttc gctgggtatc
     5581 gactggccga aagacaagct gaacatctgg atcctcgatg acggcggcag ggaagagttt
     5641 cgccagtttg cgcaaaacgt gggggtgaaa tatatcgccc gtaccactca tgaacatgcg
     5701 aaagcgggca acatcaacaa tgcgctgaaa tatgccaaag gcgagttcgt gtcgatattc
     5761 gactgcgacc acgtaccaac gcgatcgttc ctgcaaatga ccatgggctg gttcctgaaa
     5821 gagaaacagc tggcgatgat gcagacgccg caccacttct tctcgccgga tccgtttgaa
     5881 cgcaacctgg ggcgttttcg taaaacgccg aacgaaggca cgctgtttta tggtctggtg
     5941 caggacggca atgatatgtg ggatgccact ttcttctgcg gttcatgtgc ggtgattcgc
     6001 cgtaagccgc tggatgagat tggcggtatt gctgtcgaaa cggtaaccga agatgcgcac
     6061 acttctctgc gtctgcaccg tcgtggctac acctcagcgt atatgcgtat tccgcaggca
     6121 gcggggctgg cgaccgaaag tctctcggcg catatcggtc agcgtattcg ctgggcgcgc
     6181 gggatggtgc aaatcttccg tctcgataac ccgctcaccg gtaaagggct gaagtttgct
     6241 cagcggctgt gctatgtcaa cgccatgttc cacttcttgt cgggcattcc acgactgatc
     6301 ttcctgactg cgccgctggc gttcctgctg tttcatgcct acatcatcta cgcgccagcg
     6361 ttgatgatcg ccctgttcgt gctaccgcat atgatccatg ccagcctgac taactcgaag
     6421 atccagggca aatatcgcca ctctttctgg agtgaaatct acgaaacggt gctggcgtgg
     6481 tatatcgcac caccgacgct ggtggcgctg attaacccgc acaaaggcaa atttaacgtc
     6541 accgccaaag gtggactggt ggaagaagag tacgtcgact gggtgatctc gcggccctac
     6601 atcttccttg ttctgctcaa cctggtgggc gtagcggttg ggatctggcg ctacatctat
     6661 ggtccgccaa ccgagatgct caccgtggtc gtcagtatgg tgtgggtatt ctacaacctg
     6721 attattctcg gcggcgcagt cgcagtatcg gtagaaagca aacaggtacg ccgatcgcac
     6781 cgcgtggaga tgacgatgcc cgcagcaatt gcccgcgaag atggtcacct cttctcgtgt
     6841 accgttcagg atttctccga cggtggtttg gggatcaaga tcaacggtca ggcgcagatt
     6901 ctggaagggc agaaagtgaa tctgttgctt aaacgcggtc agcaggaata cgtcttcccg
     6961 gctcaggtgg cgcgcgtgat gggtaatgaa gttgggctga aattaatgcc gctcactacc
     7021 cagcaacata tcgattttgt gcagtgtacg tttgcccgtg cggatacatg ggcgctctgg
     7081 caggacagct acccggaaga taagccgctg gaaagtctgc tggatattct gaagctcggc
     7141 ttccgtggct accgccatct ggcggaattt gcgccttctt cggtgaaggg catattccgt
     7201 gtgctgactt ctctggtttc ctgggttgta tcgtttattc cgcgccgccc ggagcggagc
     7261 gaaacggcac aaccatcgga tcaggctttg gctcaacaat gatgataacg cgatgaaaag
     7321 aaaaatattc tggatttgtg cagtggctct ggggatgagt gcgttcccct ctttcatgac
     7381 gcaggcgacg ccagcaacgc aaccactgat caatgctgag ccagctgtag ccgcccaggc
     7441 ggagcaaaat ccgcaggtgg ggcaagtgat gccgggcgtg cagggcgcag atgcgccagt
     7501 cgtggcgcag aacggtcctt cccgtgatgt gaagctgacc tttgcgcaaa ttgcgccgcc
     7561 gccgggcagc atggtgctac gtggcattaa cccgaacggc agcattgagt ttggtatgcg
     7621 cagcgatgaa gtggtgacga aggcgatgct caacctcgaa tacacgccat cgccatcatt
     7681 actgcctgtc cagtcgcagt taaaggttta tctcaatgat gaactgatgg gcgtgctgcc
     7741 agtgaccaaa gaacagttgg gtaaaaaaac gctggcacaa atgcccatta acccgctgtt
     7801 tattactgat ttcaaccgtg tgcggctgga gtttgtcggc cattatcagg acgtgtgcga
     7861 aaacccggcc agcaccacgc tttggctgga tgttgggcgg agcagtggac tggatctgac
     7921 ctatcagacc ctgaatgtga agaatgacct gtcacacttc ccggtgccat tctttgaccc
     7981 gcgtgataac cgcaccaaca ccttgccgat ggtctttgcg ggtgcgccgg atgttgagct
     8041 gcaacaagca tctgccattg tcgcctcgtg gtttggttcg cgttctggct ggcgtgggca
     8101 gaacttcccg gtgctctata accaactgcc ggatcgcaat gccattgtct ttgccaccaa
     8161 tgacaaacgc ccggacttcc tgcgcgatca tccggcggta aaagccccgg tgattgagat
     8221 gattaaccat ccgcagaatc cttacgtcaa actgctggtg gtgtttggtc gtgacgacaa
     8281 agacctgttg caggcagcga aaggtatcgc tcagggtaac attctgttcc gtggtgaaag
     8341 tgtggtagtg aatgaagtga aaccgctgct accgcgtaaa ccgtacgatg cgccgaactg
     8401 ggtgcgtacc gatcgtcctg tcacctttgg tgaactgaaa acctatgaag aacaattaca
     8461 atccagcggt cttgagccag cagcgattaa cgtttcgcta aacctgccgc cggatctcta
     8521 cctgatgcgc agtaccggca ttgatatgga tattaattac cgctacacca tgccgccggt
     8581 gaaagacagt tcgcggatgg atatcagcct gaataaccag ttcctgcaat ccttcaacct
     8641 gagcagcaaa caggaggcga accgcctgct gctgcgtatt ccggtattac aaggtttgct
     8701 ggatggcaaa acggatgtct ctattccggc gctgaaactg ggcgcgacca accagttgcg
     8761 gttcgacttt gagtatatga acccgatgcc gggcggttcg gtggataact gtattacctt
     8821 ccagccggtg cagaatcatg tggtgattgg tgacgactcc accatcgact tctcgaagta
     8881 ttaccacttc atcccgatgc cggatctacg cgcctttgct aacgcgggct tcccgttcag
     8941 ccggatggcg gatttgtcgc aaaccatcac cgtgatgccg aaaacgccaa acgaagcaca
     9001 gatggaaacc ttgctgaata ccgttggctt tatcggtgcg cagacgggct tcccggcgat
     9061 taacctgacg gtgaccgatg atggcagcac cattcagggc aaagatgccg acatcatgat
     9121 cgtcggtggt atcccggaca aactgaaaga cgataagcag atcgacctgt tggtgcaggc
     9181 gaccgaaagc tgggtgaaaa cgccgatgcg ccagaccccg ttccccggta ttgtgccgga
     9241 cgagagcgat cgcgcggcag aaacccagtc aacgctgacc tcttccggtg cgatggcggc
     9301 ggtgattggc ttccagtcgc cgtataacga ccagcgcagc gtgattgcgc tgctggcaga
     9361 tagcccacgc ggttatgaaa tgcttaacga tgcggtgaac gatagcggca aacgcgccac
     9421 catgttcggt tcggtcgcgg tgatccgcga gtccggtatc aacagcctac gtgttggcga
     9481 tgtttattac gtaggtcatc tgccgtggtt cgagcgcttg tggtatgcgc tggcaaacca
     9541 tccgattctg ctggcggtgc tggcggctat cagtgtgata ttgctggcgt gggtactgtg
     9601 gcgtctgctg cgtattatta gtcgtcgtcg tcttaacccg gataacgagt aattgaagat
     9661 gaatgtgttg cgtagtggac tcgtgacgat gctgctgctg gctgccttta gtgttcaggc
     9721 agcctgtacc tggcctgcct gggagcagtt taaaaaggat tacatcagtc aggaagggcg
     9781 cgtcatcgac cccagcgacg cgcgcaaaat caccacctcc gaagggcaaa gttacggcat
     9841 gttctttgcc ctggcggcta acgaccgtgc agctttcgat aatcttctcg actggacgca
     9901 gaacaatctc gctcagggtt ctttaaaaga aaatttgccc gcctggctgt ggggcaagaa
     9961 agagaacagt aagtgggaag tgctggacag caattcggcc tccgatggtg atgtctggat
    10021 ggcctggtcg ttgctggagg cggggcgttt gtggaaagag cagcgttata ccgacatcgg
    10081 cagcgcattg ctaaaacgta tcgcgcggga ggaagtggtg acggtgcctg ggctgggctc
    10141 catgttgtta ccgggcaaag tgggttttgc tgaggataac agctggcgtt ttaaccccag
    10201 ctacctgccg ccgacgctgg cgcagtattt cacccgcttt ggcgcgccgt ggaccacgct
    10261 gcgcgaaacc aatcaacgtt tattgctgga aaccgccccg aaaggctttt cgccagactg
    10321 ggtgcgctat gagaaagaca aaggctggca gctaaaagcc gaaaaaacgt tgatcagcag
    10381 ctacgacgct atccgcgttt acatgtgggt aggcatgatg cctgacagcg atccgcaaaa
    10441 agcgcggatg ctcaaccggt ttaaaccgat ggcgacattc actgagaaaa acggttatcc
    10501 gccggaaaaa gtggatgtgg ctacggggaa agcgcagggt aaaggaccgg tcggtttttc
    10561 tgccgccatg ctgccctttt tacaaaaccg cgatgcgcag gccgttcagc gccagcgcgt
    10621 ggccgataac tttcccggca gcgatgccta ttacaactat gtgctgaccc tgtttggaca
    10681 aggctgggat caacaccgtt tccgcttctc gacaaaaggt gagttattac ctgactgggg
    10741 ccaggaatgc gcaaattcac actaaacata ttcacgcttt ccctcggtct ggccgtcatg
    10801 ccgatggtcg aggcagcacc aaccgctcag caacagttgc tggagcaagt tcggttaggc
    10861 gaagcgaccc atcgtgaaga cctggtgcaa cagtcgttgt atcggctgga acttattgat
    10921 ccgaataacc cggatgtgat tgctgcccgt tttcgttctc tgttacgtca gggcgatatt
    10981 gatggcgcac aaaaacagct cgatcggctg tcgcagttag cgccgagttc aaatgcgtat
    11041 aaatcgtcgc ggactacgat gctgctttcc acgccggatg gtcgtcaggc actgcaacag
    11101 gcaagattgc aggcaactac cggtcatgcg gaagaagctg tggcgagtta caacaaactg
    11161 ttcaacggtg cgccgccgga aggtgacatt gctgtcgagt actggagtac ggtggcgaaa
    11221 attccggctc gccgtggcga agcgattaat cagctaaagc gcattaatgc ggatacgccg
    11281 ggcaacacgg gcctgcaaaa caatctggcg ctattgctgt ttagtagcga tcgccgtgac
    11341 gaaggctttg ccgtcctgga acaaatggcg aaatcgaacg ccgggcgcga aggggcctct
    11401 aaaatctggt acgggcagat taaagacatg cccgtcagcg atgccagcgt gcaggcactg
    11461 aaaaaatatc tctcgatctt tagcgatggc gatagcgtgg ctgctgcgca atcgcaactg
    11521 gcagaacagc aaaaacagct ggccgatcct gctttccgcg ctcgtgcgca aggtttagcg
    11581 gcggtggact ctggcatggc gggtaaagcc attcccgaac tacaacaggc ggtgcgggcg
    11641 aacccgaaag acagtgaggc tctgggggcg ctgggccagg cgtattccca gaaaggtgat
    11701 cgcgccaatg cagtggcgaa tctggaaaaa gccctcgcgc tggatccgca caacagcaat
    11761 aacgacaaat ggaacagtct gctgaaagta aaccgctact ggctggcgat ccagcagggc
    11821 gatgccgcgc tgaaagccaa taatcctgac cgggcagaac ggctgttcca gcaggcacgt
    11881 aatgtcgata acaccgacag ctatgcagtg cttgggctgg gcgatgtggc gatggcgcgc
    11941 aaagattatc ccgccgccga acgctattac cagcagacct tgcgtatgga cagcggcaac
    12001 actaacgccg tgcgcgggct ggcgaatatt taccgccagc agtcgccaga aaaagccgaa
    12061 gcgtatatcg catcgctctc tgccagccag cggcgcagca ttgatgatat cgaacgcagc
    12121 ctgcaaaacg accgtctggc acagcaggca gaggcactgg aaaaccaggg gaaatgggcg
    12181 caggcggcag cacttcagcg gcaacgactg gcgctggacc ccggcagtgt atggattact
    12241 taccgacttt cgcaggatct ctggcaagcc ggacaacgca gccaggccga tacgttaatg
    12301 cgcaatctgg cgcagcagaa gccgaacgac ccggagcagg tttacgctta cgggctgtat
    12361 ctctccggtc acgaccagga cagagcggcg ctggcgcata tcaacagcct gccgcgcgcg
    12421 cagtggaaca gcaatattca ggagctggtt aatcgactgc aaaacgatca ggtgctggaa
    12481 accgctaatc gcctgcgaga aaacggtaaa gaggcagaag cggaagcgct gctgcgccag
    12541 caaccgcctt cctcgcgtat tgacctcacg ctggctgact gggcgcagca gcgacgtgat
    12601 tacaccgccg cccgcgcggc ttatcagaac gttttgacgc gcgagcccac taacgccgat
    12661 gccattcttg gtctgacgga agtggatatt gctgccggtg acaaagcggc ggcacgtagc
    12721 cagctggcga aactgcctgc cactgataac gcctcgctga acacacagcg gcgcgtggcg
    12781 ctggcacagg cgcagcttgg cgataccgca gcggcgcagc agacatttaa taagttgatc
    12841 ccgcaggcaa aatctcagcc accgtcgatg gaaagcgcga tggtactgcg tgatggtgcg
    12901 aagtttgaag cgcaggcggg cgatccaatg caggcgctgg aaacctacaa agacgccatg
    12961 gttgcatccg gtgtgaccac gacgcgtccg caggataacg acacctttac ccggctgacc
    13021 cgtaacgacg agaaagatga ctggctgaaa cgcggcgtgc gcagcgatgc ggcggacctc
    13081 tatcgccagc aggatcttaa cgtcactctc gagcacgatt actggggttc gagcggcacc
    13141 ggtggttact ccgatctgaa agcgcacacc actatgttgc aggtggacgc gccctattct
    13201 gacgggcgga tgttctttcg cagtgatttc gtcaatatga acgtcggcag tttctccact
    13261 aatgccgatg gcaaatggga tgacaactgg ggcacctgta cattacagga ctgtagcggc
    13321 aaccgcagcc agtcggattc cggtgccagc gtggcggtcg gctggcgaaa tgacgtctgg
    13381 agctgggata tcggtaccac gccgatgggc ttcaacgtgg tggatgtggt cggcggcatc
    13441 agttacagcg atgatatcgg gccgctgggt tacaccgtta acgcccaccg tcggcctatc
    13501 tccagttctt tgctggcctt tggtgggcaa aaagactctc cgagcaatac cgggaaaaaa
    13561 tggggcggcg tgcgtgccga cggcgtgggg ctaagtctga gctacgataa aggtgaagca
    13621 aacggcgtct gggcatcgct tagcggtgat cagttaaccg gtaaaaatgt cgaagataac
    13681 tggcgcgtgc gctggatgac gggctattac tataaggtca tcaaccagaa caatcgccgc
    13741 gtcacaatcg gcctgaacaa catgatctgg cattacgaca aagacctgag tggctactca
    13801 ctcggtcagg gcggttacta cagtccgcag gaatacctgt cgtttgccat accggtgatg
    13861 tggcgggagc gcacggaaaa ctggtcgtgg gagctggggg cgtctggctc gtggtcgcat
    13921 tcacgcacca aaaccatgcc gcgttatccg ctgatgaatc tgatcccgac cgactggcag
    13981 gaagaagctg cgcggcaatc caacgatggc ggcagcagtc agggctttgg ctacacggcg
    14041 cgggcattac ttgaacgacg tgttacttcc aactggtttg tcggcacggc aattgatatc
    14101 cagcaggcaa aagattacgc acccagccat ttcctgctct acgtacgtta ttccgccgcc
    14161 ggatggcagg gtgacatgga tttaccaccg cagccgctga taccttacgc cgactggtaa
    14221 gttttcagat agcgcctctc ttaatgccgc tgcgatcggg tatactcggg cggcaatctg
    14281 ggatttccgg ggggagacaa tttgcgcgta agtcgctcgt taacaatcaa gcagatggca
    14341 atggtggcag ccgttgtcct ggtgttcgtt tttatttttt gcaccgtttt gctgttccat
    14401 ctggtccagc agaatcgcta taacacggct acgcaactgg aaagcattgc tcgctctgtc
    14461 cgcgaaccct tatcttctgc cattttgaaa ggcgatattc ccgaagcgga agctattctt
    14521 gccagcatta aaccggcagg cgtggtcagc cgtgccgatg tagtgctgcc caaccagttc
    14581 caggcgctgc gtaaaagttt tattccagag cgtccggtgc cggtaatggt tactcgcctg
    14641 tttgagctac cggtgcaaat ctcgctgggc gtttactcgc tggaacgtcc ggcaaacccg
    14701 cagccaattg cctatctggt gctacaggcg gattccttcc gcatgtataa gttcgtgatg
    14761 agcaccctct caacgttagt gaccatttac ttacttttgt cgttaatatt gactgtggcg
    14821 attagctggt gcattaaccg cctgattttg catccattac gcaatattgc tcgcgaactt
    14881 aacgccatcc caccccagga tcttgttggt caccaactgg cattaccgcg tctgcatcag
    14941 gacgatgaaa tcggtatgtt ggtgcgcagt tacaacctca accagcaatt gctgcagcgc
    15001 cattatgaag aacagaacga aaatgcgatg cgcttcccgg tgtcggattt gccgaacaaa
    15061 gccttgctga tggagatgct ggagcaggtg gtggcgcgta aacaaaccac cgcgctgatg
    15121 atcatcacct gtgaaaccct gcgtgatact gcgggtgtgc tgaaagaggc gcaacgagaa
    15181 attctgctgc tgacgctggt ggaaaaactc aaatcggtac tgtcgccacg catgatcctc
    15241 gcgcagatta gcggttatga ctttgctgtc attgccaacg gtgtacagga accgtggcac
    15301 gctatcacat taggtcagca agtgctcact atcatgagcg agcgcctgcc gattgaacgt
    15361 attcaactcc gtccgcactg tagcattggc gtggcgatgt tctacggcga tctcaccgcc
    15421 gaacagcttt acagtcgcgc tatttctgcg gcatttaccg ctcgccataa aggcaaaaat
    15481 cagattcagt tctttgatcc gcagcagatg gaagccgccc agaagcggtt gacggaagag
    15541 agcgatatcc ttaatgcact ggaaaatcat cagtttgcga tttggttaca gccacaggtc
    15601 gagatgacca gcggtaaact ggtcagtgcg gaagtgttac tgcgtatcca gcaaccggat
    15661 ggcagttggg acctgccgga tggcttaatc gatcgcattg agtgctgtgg gctgatggtt
    15721 accgtcggtc actgggtgct ggaagagtcc tgtcgattgc ttgctgcctg gcaagagcgc
    15781 ggcattatgc tgcccttgtc ggtaaacctc tcagcgctgc aactgatgca tccaaatatg
    15841 gtggcggata tgctggaact gttaacccgc tatcgcattc agccgggaac actgattctg
    15901 gaagtgacag aaagccgacg tattgacgat cctcatgctg cggtggcaat cctccgtccg
    15961 ctgcgcaatg ccggagttcg ggtggcgctg gatgatttcg gcatgggcta cgcagggctg
    16021 cgtcagctgc agcatatgaa atcgttgcca atcgacgtac tgaaaatcga caaaatgttt
    16081 gttgaaggtt tgccggaaga tagcagcatg attgctgcaa ttatcatgct ggcgcagagc
    16141 ctgaacctac aaatgattgc cgaaggcgtg gagactgaag cacaacgcga ctggctggca
    16201 aaagcgggcg ttggtattgc ccagggcttc ctttttgctc gcccactccc tattgaaatc
    16261 ttcgaagaga gttacctgga agaaaaatag ctaccccaaa ctgattacaa aactttaaaa
    16321 agtgctggtt tgtgcgagcc agctcaaact ttttaacctt tttgtttcaa ttatgatcca
    16381 ggtacatttc tgtgatgttg tctgggtgtt attttaaggc cgcaggtacc ccataacctt
    16441 acaagacctg tggttttact aaaggacacc ctatgaaaac ctctctgttt aaaagccttt
    16501 actttcaggt cctgacagcg atagccattg gtattctcct tggccatttc tatcctgaaa
    16561 taggcgagca aatgaaaccg cttggcgacg gcttcgttaa gctcattaag atgatcatcg
    16621 ctcctgtcat cttctgtacc gtcgtaacgg gcattgcggg catggaaagc atgaaggcgg
    16681 tcggtcgtac cggcgcagtc gcactgcttt actttgaaat tgtcagtacc atcgcgctga
    16741 ttattggtct tatcatcgtt aacgtcgtgc agcctggtgc cggaatgaac gtcgatccgg
    16801 caacgcttga tgcgaaagcg gtagcggttt acgccgatca ggcgaaagac cagggcattg
    16861 tcgccttcat tatggatgtc atcccggcga gcgtcattgg cgcatttgct agcggtaaca
    16921 ttctgcaggt actgctgttt gccgtactgt ttggttttgc gctccaccgt ctgggcagca
    16981 aaggccaact gatttttaac gtgatcgaaa gtttctcgca ggtcattttc ggcatcatca
    17041 atatgatcat gcgtctggca cctattggtg cgttcggggc aatggcgttt accatcggta
    17101 aatacggcgt cggcacactg gtgcaactgg gacagctgat tatctgtttc tacatcacct
    17161 gtatcctgtt tgtggtgctg gtattgggtt caatcgctaa agcgactggt ttcagtatct
    17221 tcaaatttat ccgttacatc cgtgaagaac tgctgattgt actggggact tcatcttccg
    17281 agtcggcgct gccgcgtatg ctcgacaaga tggagaaact cggctgccgc aaatcggtgg
    17341 tggggctggt catcccgaca ggctactcgt ttaatcttga tggcacatcg atatacctga
    17401 caatggcggc ggtgtttatc gcccaggcca ctaacagtca gatggatatc gtccaccaaa
    17461 tcacgctgtt aatcgtgttg ctgctttctt ctaaaggggc ggcaggggta acgggtagtg
    17521 gttttatcgt gctggcagcg acgctctctg cggtgggcca tttgccggta gcgggtctgg
    17581 cgctgatcct cggtatcgac cgctttatgt cagaagctcg tgcgctgact aacctggtcg
    17641 gtaacggcgt agcgaccatt gtcgttgcta agtgggtgaa agaactggac cacaaaaaac
    17701 tggacgatgt gctgaataat cgtgcgccgg atggcaaaac gcacgaatta tcctcttaat
    17761 ctcaccactt atgcccgcac tcccttcccc gagtgcgggt gtttgcgcat aattgacccc
    17821 tgtgcctgaa cttaccacct gggtgagttc aggtttattt cacttcttcc gtgacatttt
    17881 catgttcttg cggtctaaca cgaagtgttt ttaacgtcat ttagactgac cgacaacggg
    17941 cagtttttta ttaccaggat agttgatcag gggttcacat gcagggcaca aaaattcgac
    18001 ttttagcggg cggtttgctg atgatggcca ctgctggcta tgtgcaggca gatgcgctcc
    18061 agcctgatcc agcatggcaa caggggacgc tttccaacgg tttacagtgg caagtgctga
    18121 ctacacccca gcgtcccagc gatcgtgttg aaattcgcct gctggttaat accggttcgc
    18181 tcgccgaaag tacacaacag agcggttaca gtcacgccat ccctcgtatt gcgctaacgc
    18241 aaagcggtgg ccttgacgca gcgcaggcgc gttcattgtg gcagcagggg atcgacccta
    18301 aacgcccgat gccgccggta attgtctctt atgacaccac gctgtttaat ctgagtttgc
    18361 ccaataaccg taacgacttg ctgaaagagg cgctctctta tctggcaaat gccactggca
    18421 aactgactat cacgccagaa accatcaacc atgcgttgca aagtcaggac atggtggcaa
    18481 cctggcctgc cgatactaaa gagggctggt ggcgttatcg tctgaaagga tcaaccttgt
    18541 taggtcacga tcctgccgat ccgctgaaac aacccgttga agcggaaaag attaaagatt
    18601 tctatcagaa atggtacacc ccggatgcaa tgacgctgct ggtggtggga aacgtggatg
    18661 cgcgctcggt cgtcgaccaa atcaataaaa cgtttggcga actgaaaggc aaacgtgaaa
    18721 caccggctcc ggtgccgacg ctttctccgc tgcgtgcgga agcggtgagt attatgaccg
    18781 acgcggtgcg tcaggaccgg ttatctatca tgtgggatac gccgtggcag ccgattcgtg
    18841 aatcagccgc actgctgcgc tactggcgtg cggacctggc ccgtgaggcg ctgttctggc
    18901 atgttcagca agcgttaagc gccagtaaca gtaaagacat cggtcttgga tttgactgtc
    18961 gtgtgctgta tctgcgtgcg cagtgtgcca tcaacatcga atcaccaaac gacaagctga
    19021 acagcaacct taatctggtg gcgcgtgaac tggcgaaggt tcgcgataaa ggtctgccgg
    19081 aagaagagtt caatgcgtta gtggcgcaaa agaaactgga gctgcagaag ctgtttgccg
    19141 cctatgcgcg agctgatacc gatattctga tggggcagcg gatgcgttcg ttgcaaaatc
    19201 aggttgtcga tatcgcgccg gagcaatatc agaaactgcg tcaggatttc cttaacagcc
    19261 tgacggtgga gatgttaaat caggatctgc gtcagcagtt gtcgaatgat atggcgttaa
    19321 tactgctgca gccgaaaggc gagccggaat ttaacatgaa agcgttgcag gcggcctggg
    19381 atcaaatcat ggccccatcg actgcggctg ccgccacatc tgtcgccacg gatgacgtac
    19441 atcctgaagt gacggatatt ccacctgcac agtaatgcct gatggtgatg agttagcaca
    19501 accatcaggt atttatggat gagctggata ggggggtaac actctatcca gccttttgca
    19561 tatgctgctt ttacgctggc atcgcttcac gcgggataat cgcgccgcga tactgaataa
    19621 cggtacttgc ggtcaggtgc ccgcgtttcg ctgcgtcttc cgcactgccg cccgtcaaac
    19681 gtaccgccag ataaccggca ctgaaagagt cgccagctgc ggtggtatcg atcacttttt
    19741 ctttcggcag ttttaccgcc ggaacatcca ctaacccttc accagcaatg gacaccaggc
    19801 aagaatccgc cccgcgtttc accaccactt ctttcacgcc cgcgttatgg gtgcgcgcaa
    19861 tgacgtcttc caccggctgt tgaccccaca gcgcgtcttc atcgtccagc gtcaggaagg
    19921 cgatatccgt gcattccagc atttgttggt acacctgctg tgtctcttct ttgctggccc
    19981 acaggcgcgg acgatagtta ttgtcgaaaa tcacttttcc gccgttggcg cggcattcgc
    20041 gcagcaggga aagcagcttt tcgcggctgg tcgggcttaa gatcgccagg ctaatcccgc
    20101 tcaggtagag ataatcgaaa ttcgccagct cttcgcaaat cgtcgcggat tgctcactct
    20161 ccagccagaa tttggcggcg gcttcgttac gccagtagta gaacgtacgt tcgccggtgc
    20221 tgtcggtttc aatgtagtaa aggcccggca gacggttttc catccgttgg gtcagggaag
    20281 tatcaacgtt ctcgccgcgc caggcgtcca gcatctgctg gctaaaactg tccgttccca
    20341 gcgccgttac gtaatgaacg gttaatgccg caggatcgac ctgacgggcg atatagacgg
    20401 aagtgttcag ggtatcgccc ccgaaaccgc gcttaacgtc cgcgcctttc tcggaaagct
    20461 caatcatgca ttcgccaatc acggcaatct ttttggacat agttgtgaac ctgatctgta
    20521 aaaaattagc gttagtttgc gctgtggtgg tttgctggtc aaccatatta aaacagtgtt
    20581 ccattatttt tttgagccag aacgtattcc tgaaagattc cgttgtggag tggggaaatt
    20641 taccccggcc aggcggtaaa gttctgcctt tgcgcgccga taatctttgt cgagtccggg
    20701 cagcatcact tttaaacaca ggacatcttt gatgataagg caggttatcc agcgaataag
    20761 caaccctgaa gcaagcatcg agagcttgca ggaacggcgt ttttggttgc agtgtgagcg
    20821 tgcttacacc tggcagccga tctatcaaac atgcgggcgg ttaatggccg tggagctatt
    20881 aacggtggtc acgcatccct tgaacccttc gcaacgcctg ccgccggatc gctattttac
    20941 tgaaatcacc gttagccatc ggatggaggt tgtgaaagag cagattgatt tgctggcgca
    21001 aaaagccgac ttctttgtag agcacggcct gctggcatcg gtcaatattg atggccctac
    21061 gctcatcgcc ctgcgtcagc aaccaaaaat cctgcgccag attgaggagc ttccctggct
    21121 gcgtttcgaa ctggtggaac acatccgcct gccgaaagat tcaacctttg cctcgatgtg
    21181 tgaatttggc ccgctgtggc tggatgattt tggtaccggg atggcaaatt tctctgcact
    21241 aagtgaagtg cgttatgact acatcaaaat cgcgcgagaa ctgtttgtga tgctgcgtca
    21301 gtcaccggaa ggacgcacac tcttttctca gcttttacat ctaatgaatc gctattgtcg
    21361 cggggtgatt gtcgagggcg tagaaacacc agaagagtgg cgtgatgttc agaactcgcc
    21421 cgcattcgcc gcacaaggct ggtttctttc acgcccggca ccgatagaaa cgctgaatac
    21481 ggcggttctg gcgctataag ctgcctcatt ttccgcctgg ctggactatc tttaggactg
    21541 gcacagaaag aatatgagga agcgaacgat gagcaaggca ggcaaaataa ccgctgcgat
    21601 ttcaggggct ttcttgttgt tgattgtcgt ggcgatcatt ttgattgcaa cttttgactg
    21661 gaatcgactc aaaccgacca tcaaccagaa agtctctgcg gagttgaatc gtccgttcgc
    21721 tatccgtggc gatctgggcg tggtgtggga gcggcaaaaa caagaaactg gctggcgcag
    21781 ctgggtgccg tggccccatg tacacgcgga agacatcatt cttggcaatc caccggatat
    21841 tcccgaagtc acaatggtgc atttgccgcg agtagaagcg acgctggccc cgctggcgct
    21901 gctgaccaaa acggtctggt tgccgtggat caagctcgaa aagcccgacg cgcgcctgat
    21961 tcgcctctcc gaaaagaaca ataactggac gtttaatctc gccaacgatg acaacaaaga
    22021 cgcgaatgca aagccgtcgg cgtggtcgtt tcggctggat aatattctgt tcgatcaagg
    22081 gcggatcgcc attgatgaca aagtaagcaa agcggatctg gaaatttttg tcgatccgtt
    22141 aggcaagccg ctgtcgttca gcgaagttac cggatcgaaa ggtaaagcgg ataaagaaaa
    22201 ggtgggcgat tacgtttttg gcctgaaggc gcagggacgt tataacggtg aaccgctcac
    22261 tggtacggga aaaataggcg gtatgctggc gctgcgtggc gaaggaacgc cgtttccggt
    22321 acaggctgat ttccgctctg gtaacacccg tgttgctttt gatggcgttg tgaatgaccc
    22381 aatgaagatg gggggtgtcg atttacggct taaattttct ggcgattcgc tgggtgatct
    22441 ctatgaactg acgggcgttc tgctgcccga tacaccgccg tttgaaacgg atggtcggct
    22501 ggtagcgaaa atcgatactg aaaaatcgtc ggtctttgat tatcgcggtt ttaatgggcg
    22561 cattggcgat agcgatatcc acggttctct gatctacacc accggcaagc cacgaccaaa
    22621 actggaaggt gatgtcgagt cgcggcaatt gcggctggcg gacctgggac cgttgattgg
    22681 cgttgattcc gggaaagggg cagaaaagtc gaaacggtct gaacagaaga agggcgaaaa
    22741 aagcgttcag cctgcgggca aagtgctgcc ttatgaccgc ttcgaaaccg ataaatggga
    22801 cgttatggat gccgatgttc gcttcaaagg gcggcgcatt gagcatggca gcagcctgcc
    22861 gattagcgat ctttctactc atatcatcct caaaaatgct gacctgcgcc tgcaaccgct
    22921 gaaatttggc atagcgggcg gcagcattgc ggcgaatatt catctggaag gcgataaaaa
    22981 gccgatgcag gggcgggcag atattcaggc tcgtcgactg aaactgaaag aactgatgcc
    23041 cgatgtagaa ctgatgcaga agacgctggg ggaaatgaac ggtgacgcgg aactgcgcgg
    23101 tagcggtaac tcggtggcgg cgcttttagg caacagtaac ggcaacctga aactgttgat
    23161 gaatgacggg ctggtgagcc gcaacctgat ggagattgtt gggctgaatg tcggcaacta
    23221 cattgtcggt gcgatatttg gtgatgatga ggtgcgggtg aactgcgcgg cggcgaatct
    23281 ggatattgcc aacggcgtgg cgcgcccgca gatttttgct ttcgatactg agaacgcgtt
    23341 gattaacgtt accggtacgg caagttttgc ttcggaacag ctggatttga ctattgatcc
    23401 ggagagtaaa gggattcgga ttatcacatt gcgttcgccg ctgtatgtac gtgggacgtt
    23461 taaaaatccg caggctgggg tgaaagccgg gccgttgatt gctcgcggag ccgttgcggc
    23521 ggcactggca acgctggtaa caccggcggc ggcgttactg gcgctgatct caccttccga
    23581 aggggaggct aatcagtgca ggacaatttt gtcgcagatg aagaagtgat gataattgcc
    23641 ggatgtctga catccggcaa ttaaacagat ttagattgtt ggcaaagtgt gctttgttca
    23701 tgccggatgc ggcgtgaacg cctccggctt acaaatcctt gcgaattcaa tatattgtat
    23761 ggaaattctg gcctgataag cgtagcgcat caggcttttc actcttatta caacgactga
    23821 tgtcgcgtct catgggtcag cagcagggcg attaacgtca agccagccat cgccgccaga
    23881 tatagcccca ccgcacctaa cccgtagtta gtctgcaacc aggctgcgat atacggcgca
    23941 acggaagccc cgagaatcga cgatacgttg taagagaacg atgctccggt gtaacgcact
    24001 tctgtcggga acagctctgg taacagcgcg cccatcggac cgaaggtcag ccccatcaga
    24061 cttaacccca gcagcagaaa ggcaaaaacc agaatcgggt tgccagaacc gagcaaaggg
    24121 gtaaaggcga atagcgcgaa caggatgatc agcgtggtga tgatcaccat gcttttacgg
    24181 cgaccaaagg catcagccag taatccggcg actggcacca tcacgccaaa accaataact
    24241 gccatcatca gcatccacaa cacttcgtta cgcggcaggc caagcccaac tggcgcggcg
    24301 gcggtactaa aggtcataga gtagaccgtc atgatgtaaa acagcgtata ggttgccagc
    24361 ataatgaacg tacccagtac ggttacgcga acatgtttgg tcagcagcgt acccagcggg
    24421 atcttcacct gttttttcgc tttagcgact ttctcaaaca ccggcgactc atgcagcgac
    24481 acgcgaacat acaggccgat aatgaccagc accgccgaga agataaatgg cacacgccag
    24541 ccccagctca taaactgctc gtcggtcagt agccaggaaa gcagcaagaa agtgccgttg
    24601 gcaaagaaga agccaatcgg tgcgcccagc tgcgggaagg agccgtacaa tgcacgtttg
    24661 cgcggtgggg cgttttcagt cgccagcagc gccgcgccgc cccattcacc acctaagccc
    24721 agaccctgac caaatcgagc cagcgccagc agcagcgggg cgaaaatacc aatcgtggca
    24781 tagcctggca gcagaccaat caccacggtc gaaatcccca tcgttagcaa cgaggcgacc
    24841 agcgtcgctt tacgcccaac gcgatcgcca aaatgaccaa aaacggcaga gccaatgggg
    24901 cgcgcgacga aggcgatggc gaaggtggcg agcgactgta gcgttgctgc cgttggatcg
    24961 ccctgcggga agaagatatg cggaaacaca ataacggccg cagtggcgta aatgtaaaag
    25021 tcgaagaact caatggccgt gccaatgaga gaggcgacaa ggactttatt acgcgagttg
    25081 atcggcgtgt attcttgctc gtggtcgagt gttgtggctg ttgcttgcat aatcttttct
    25141 tattttttgg ctaacgaata gccatattac gcacagcaaa agtgatattt caatgtgtaa
    25201 caaatcgcgg aaatggtcaa aaatcaggca aaaagggaat aatgtttagg ccagtgattt
    25261 ttcttctatg aaatgcttca cataatttta taaatggtgg attaaactgg ttttaggtta
    25321 aataaaagtt accaactgga tttatatgct ggggttatga gttggaatga aattgtttgc
    25381 tcctgaatag atggctaaag aggctctggg aaagagtttc aagaggttat gtgcgcatta
    25441 tagccggatg cgatgctggc gcatcttatc cggcctacgg ggagccaggc cggataaatt
    25501 cgtacgccgc atccggccta ttttaaggct gtgttttccc cggcattcgc gggtcgtctt
    25561 tatattcggc ggtggcaatc cacgccgcgc aaaacagcgt caaacgggcg aagaagtaga
    25621 aaaatgccat cagccccagc acggaaccaa atgctgcgcc agagggtgat ttcatcaacg
    25681 atggcagggt gtaggtcatc acgattttaa tcacttcaaa accaatagca gccagaaatg
    25741 ttccgcggat cagggctttt ttgcgcggac ggtggcgcgg caaccgccag aagatccaga
    25801 agaaaagcag atagttggcg aagatggaaa tcgccacgcc aatcaaccgc catgtcggtt
    25861 tcagccactc aatgctgttc aggtgcaggg cgctaataat catttgctgc gcagaaccgg
    25921 caaccgaggt gatagaaagc gtcacaatca gcgcaatcaa caaaccaatc agcgaaataa
    25981 aatcacgcag atattttacc cagaacttct cctgatcttg cggcgagcgt tcccagacat
    26041 cgcgtgactg ggcgcgaatc gcttcacgca ggttacccat ccagttgatg ccggaataaa
    26101 gtgccactgc cagaccgaca agccctacag tcgtacgctg ctgaacggcg gtgttgatgg
    26161 tgtttttcaa tgtggcggct agcgtcggat cgctgatgtt ttgcagaatt ttgtcgaaga
    26221 tatcctgtag caacatcgga tgggaggcga gcacaaagcc ccctgcggca aacgacacca
    26281 tcaaaatggg gatcatcgac aaaaatgaga aataggtgat agccgcacca aactggttac
    26341 ccagccgatc gttaaagcgt tctgtcgcgc gaatcaggtg cgcaataacc ggttggcgtt
    26401 ggactttctc ggcagtggtg gtgacggttt ccagcgcctg gctaactttg ccgcctttct
    26461 cgctattatc caactgctta atcggctcgt gctccagatc ctgggtggga cgtttgatct
    26521 cgttttcctg cgtcatccgc tcttttgtcc ttctgtcgtg gtcattctta aaagtatagt
    26581 cggtcagtcc aggtactctt tcattacgcc agccagccac tgcataaaca ggtttacacg
    26641 ccgggaaagc tcccgacgct gcggataaac cagtgaaagg gacagcggct cggcacggta
    26701 gccaggcaac acttcaataa gccgcccggc acgcagggct tcgcgcacgg caatgcgcgg
    26761 gatctgaata atccccagac cggcaagacc ggcggcgaga tagttttcgc tactgtttac
    26821 cgtcaacatg ctgccagact taaaccactg gacgccatta acgctggcaa cctcaaaacc
    26881 taacggatgt acacccaggt gcggtgtata acgcactatt gcgtgtgaag tcagatcatc
    26941 ggggctttgc ggataaccaa agcgcgtcag atagtgcgga ctggcacagt tgaccatggt
    27001 cagtttaccg aggggacggg cgataacgcc atcttccggt aatgcgccag tgcgtatcac
    27061 gcaatcaaaa ccatcatgaa gaatatctac cggacggtca tggctactca gttccagctc
    27121 aattcccgga tgcagataga gaaattccga caagcgcggc agtaacaggc ttttcgcgat
    27181 tcctgacggg atgtcgatgc gtaatttacc gctgatactg gtggcatcct gttgaaacag
    27241 accgtccagt tcgctgagat tactcaacac atcttttgct cgttgataat aggtcatgcc
    27301 ttctggcgtg agtttgaccc gtcgcgtggt gcgctggaga agctgggtgc caagttgatg
    27361 ttccagtgcc tgtatctggc gcgaaacact tccctttggc aaagcaaaga aatccgccgc
    27421 gcgggaaaaa ctttccagct ccgcgacttt gatgaacaac tgcattgcgt gaattttatc
    27481 catttctttg accgattgtt gttcacaacg aaacagtagc gtgcatatcg cattgtttat
    27541 tgttttttct aacatctaat gccgggtttc acaatgttgt catgatgaat gtggcgattt
    27601 ctcagcagga aaagggagga atagctttat gagaaaggta aattccctta aacagagcgt
    27661 atttttcagc gattcccttt aaagtgaata gaagatgtat tggctatttg tgcttatttt
    27721 tatctcattg ccgtttgtta tttttgttta tggtcgcgga atttcatttt taattgcatg
    27781 cgtactaact accgatataa ggaaatcctc aaaaatgttt taaatagtgc cctcgacatg
    27841 taatttcagg cgtttagctg taactattta tatcggtaac tattttctac gtgtcatctc
    27901 tgtagaatgg ccaccgccaa atggcgaatt cttatttttt ctcgcgggta atacccttcc
    27961 actacttaag tctttttaac gtattcaggt ggagattcag ttatgcaaat agtcatgttt
    28021 gacaggcagt caatatttat tcatggaatg aaaatcagtt tacagcatcg tattccagga
    28081 gtgagtattc agggggccag tcaggcagac gagttatggc aaaagctgga aagttaccct
    28141 gaagccttag tgatgctcga tggcgatcag gatggtgagt tttgctactg gttgctgcaa
    28201 aaaactgtgg tgcaatttcc tgaggttaag gtgttaatta cggcgacgga ttgcaacaaa
    28261 cggtggttac aggaagttat tcattttaat gtgctggcca ttgtgcctcg tgattcaacc
    28321 gtcgagacat ttgcgttggc ggtaaatagt gcggcgatgg ggatgatgtt tctgcctgga
    28381 gactggcgaa cgacgccgga aaaggacatt aaagatctca aatcgttaag cgcccgccag
    28441 cgggagattt taaccatgtt agcggcgggc gaatcaaata aagagatcgg cagggcgctg
    28501 aatatcagta ccggaacggt aaaagcgcat ctggaatcac tgtatcgccg tctggaagtc
    28561 aaaaatcgca cccaggcggc aatgatgtta aatatctcct cctgaagagg ataattttac
    28621 agccagcagg aagttagcgg ctggctgtaa aaatattatg gttcgccgta caaaccaatt
    28681 aaacggcgta ccacaccgtt agtccagcca aacccatcct gcaacggata ctcgccaccg
    28741 ccgccttcgc gaggaacacc atcggcaata tggtatttct cgatcagttt gtgctgttcc
    28801 agatagaact gattcaccgt cttcagccag ttgcgcgcga tttcatcacc cagaaggtca
    28861 tcgccgtaca tcttaaatcc ctgaattgcc atccactgta acggtgccca gccgttgggt
    28921 ttatcccact gttcaccggt ttcgtactcg cttgccagaa tcccgccagg tgtcagtaac
    28981 cggctgcgca cggcgtttgc cagacgatcg gcctgttcat ggttcgccat accgacatag
    29041 agcggcacaa tggcggcagc ggaaaacagc gccagttgct cgcgtcgcca gtcgtaatcg
    29101 cggtagatac cgttttcatc atcccagagg taacggttta ccgcatcgcg acgggcactg
    29161 gccttctggc ggaacagtgc ttctgtctct ttctcgcctt tcagtgccga gatgttggcg
    29221 atggcgctct ccagtttaaa caggaaggca ttcagatcga tggggatgaa ctgggtggta
    29281 cgaatgctcg ccagacgacc agtatcacgc agccaacggg aagagtaatc ccaaccggag
    29341 gccgcccccg cacgtaaatc gcggtacacc tcgttaggcg ggcgaccaga atgtttcgcg
    29401 gtttcaacgt cctcaagcca ggattcgtca cgcggcgtgt cgcgatcgtc ccagtaacgg
    29461 ttgagcagcg agccgtccgg catccgcaca acatggcgat aggcctgatt agggatcaac
    29521 gattctgcac cgtccatcca gaaggcatat tccattttca ggtggtcgag atagcggcgc
    29581 gcgccgcgta caccatcttc ttcaaacaac tccaccatca gcgcaaaaac cggtggttgc
    29641 gagcggctca aataataggt gcggttgccg ttggggatgt ggccgtagtt ttcgatcatc
    29701 caggcgaagt tatcggccat gcatttcagc aaatcttccc gaccactttc cgccagcccc
    29761 agcatggtga aataggaatc ccaatagtag gtttcgctaa aacggccgcc cgggacaata
    29821 tatgactgcg gcagcgccag cagagaagac cacggaatgt gatcctgtgg ttcacgggtt
    29881 agcaccggcc acagctggtc gatatgctct ttcagggaat tttgcgggtc cgatacatac
    29941 tcgctggagt agacctccgg cagccagaag tggttttcaa caaacttgcg caagtcaaaa
    30001 tcacgatgac ggcgcacttt acggtagcgg attaaaatat ccagcgggtc cattttgggt
    30061 gcgcagtcgg gaaaggtttt actgtcgggg aaaattttcg ccgactgaac atgctcgaac
    30121 agttcgagat agcgatcggc aggcgtcagc gcatcagagg cgggcagtcc ttcaatcatc
    30181 tcgggttccg gttctgcctc gatcatctca tccagtttta attcatacgg gtccagctca
    30241 tagcagagat cgacctcgat catcagttcg tctggattag ggttttgaat tttctgattg
    30301 agcataagga tccgacctcc gaaattcgtc gctaattatc gggcttttct ttaagggtag
    30361 tcattcgctc cgcctggtgg gcgacaaagc gtgcggtgga tcacgtttat taacctgcca
    30421 aaaaatataa cgcactaata tcaatggaat ttataaaaat acggatttcg tgttgttttt
    30481 cattttcaga acattcaatc ctgctctcgc cgcaatcatg atatgccatt cctgatctgg
    30541 gtaataggca ctggctatct tattgatagt ttatattcat gtaattgatt gattcctatc
    30601 aaaaagcata tctccctgtt ttttatgatg gctttgtctc gcagagacgt ttgcagcatt
    30661 ctggaaaatt ttctgggtca caataactca ccaccgtgag ggaagaataa tgaaaatggt
    30721 ctcacgtatt accgcgatcg gcctggctgg cgtcgcgatt tgctatttag ggttatctgg
    30781 ttatgtgtgg taccacgata ataaacgcag taaacaggcc gatgttcagg catctgctgt
    30841 cagtgaaaat aataaggttt taggctttct ccgtgaaaaa ggatgcgact attgccatac
    30901 accttcggca gaattacccg cctattatta tattcccggc gcgaaacagt tgatggatta
    30961 cgacataaag cttggatata aatcttttaa cctcgaggcc gtgcgtgcgg ctctgctggc
    31021 taataaaccc gtttcgcaaa gcgatctgaa taagattgaa tgggtgatgc agtatgaaac
    31081 tatgccgcca acgcgttata ccgcgctaca ctgggcgggt aaggtgagtg atgaagagcg
    31141 ggcggaaata ctggcctgga ttgcaaaaca gcgcgcggaa tattacgcca gcaatgatac
    31201 tgctccggag catcgcaatg aaccggtgca gcccatcccg caaaaactgc ctaccgatgc
    31261 gcaaaaagtg gcgttgggtt ttgcgctgta tcacgatccc cgtttatcgg cggatagcac
    31321 catttcatgc gctcattgcc atgcgttgaa tgcggggggc gtcgatggca gaaaaacatc
    31381 gattggtgtt ggtggcgcag ttgggccgat taacgcgccg acggtattta actcagtatt
    31441 taacgttgag cagttctggg atggtcgtgc ggcaacattg caggatcagg ctggtggacc
    31501 gccgttgaac ccgattgaaa tggcgtcgaa atcctgggac gaaattattg ctaagctgga
    31561 aaaagatccg cagcttaaag cgcagttcct cgaagtctat ccgcaaggtt tcagtggcga
    31621 aaatattact gatgccattg ctgaatttga gaaaacatta attacgccgg attccccatt
    31681 tgataaatgg ttgcgtggag atgagaatgc tctgacggcg caacagaaaa aaggctatca
    31741 attatttaaa gataataaat gtgcaacttg tcatggtggt attattctcg gcggacgttc
    31801 ctttgaaccg ttggggctga aaaaagactt taactttggt gaaattacgg cggcggatat
    31861 tggtcgtatg aatgttacta aagaagagcg tgataaattg cgtcagaaag tacccggttt
    31921 acgtaacgtt gctttaacgg caccgtactt ccatcgcggt gacgtgccga cgctggacgg
    31981 ggcagtgaaa ctgatgctgc gctatcaggt aggcaaagag ctgccgcagg aggatgtgga
    32041 tgatatcgta gctttcctgc acagtctgaa cggggtttac atgccgtata tgcaggataa
    32101 acaataatta atttgatcgc ccgaacagga gtgtttgggc gatttttatt acgataataa
    32161 agtctgtttt taatattatc atattaaatg tttatattat aaaaggt
//
LOCUS       DAHHTG010000046        32205 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.046, whole genome shotgun sequence.
ACCESSION   DAHHTG010000046 DAHHTG010000000
VERSION     DAHHTG010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 32205)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32205)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32205
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.046"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            complement(463..552)
                     /locus_tag="NBI74_002094"
     tRNA            complement(463..552)
                     /locus_tag="NBI74_002094"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(516..518),aa:Ser,seq:cga)
     gene            646..1443
                     /gene="mtfA"
                     /locus_tag="NBI74_002095"
     CDS             646..1443
                     /gene="mtfA"
                     /locus_tag="NBI74_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DgsA anti-repressor MtfA"
                     /protein_id="HCD1932436.1"
                     /translation="MIKWPWKVQESAHQTALPWQEALSIPLLTCLTEQEQSKLVALAE
                     RFLQQKRLVPLQGFELNSLRSCRIALLFCLPVLELGLEWLDGFHEILIYPAPFVVDDE
                     WEDDIGLVHNQRIVQSGQSWQQGPIVLNWLDIQDSFDASGFNLIIHEVAHKLDTRNGD
                     RASGVPFISLREVAGWEHDLHAAMNNIQEEIELVGENAASIDAYAASDPAECFAVLSE
                     YFFSAPELFAPRFPSLWQRFCQFYQQDPLQRLHHANDTDSFSATNVH"
     gene            1544..1619
                     /locus_tag="NBI74_002096"
     tRNA            1544..1619
                     /locus_tag="NBI74_002096"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1577..1579,aa:Asn,seq:gtt)
     gene            1781..3043
                     /locus_tag="NBI74_002097"
     CDS             1781..3043
                     /locus_tag="NBI74_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCD1932437.1"
                     /translation="MSLTDAKIRTLKPSDKPFKVSDSHGLYLRVKPGGSRHWYLKYRI
                     SGKESRIALGAYPAISLSDARQQREGIRKILALNINPVQQRAAERGSRTPEKVFKNVA
                     LAWHKSNRKWSQNTADRLLASLNNHIFPVIGNLPVSELKPRHFIDLLKGIEEKDLLEV
                     ASRTRQHLSNIMRHAVHQELIDTNPAANLGGVTTPPVRRHYPALPLERLPELLERIGA
                     YHQGRELTRHAVLLMLHVFIRSSELRFARWSEIDFTNRVWTIPATREPIIGVRYSGRG
                     AKMRMPHIVPLSEQSIAILKQIKDITGNNELIFPGDHNPYKPMCENTVNKALRVMGYD
                     TKKDICGHGFRAMACSALMESGLWAKDAVERQMSHQERNTVRMAYIHKAEHLEARKTM
                     MQWWSDYLEACRESYAPPYTIGKNKFIP"
     gene            complement(3237..4541)
                     /gene="ybtS"
                     /locus_tag="NBI74_002098"
     CDS             complement(3237..4541)
                     /gene="ybtS"
                     /locus_tag="NBI74_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="yersiniabactin biosynthesis salicylate synthase
                     YbtS"
                     /protein_id="HCD1932438.1"
                     /translation="MKISEFLHLALPEEQWLPTISGVLRQFAEEECYVYERQPCWYLG
                     KGCQARLHINADGTQATFIDDAGEQKWAVDSIADCARRFMAHPQVKGRRVYGQVGFNF
                     AAHARGIAFNAGEWPLLTLTVPREELIFEKGNVTVYADSADGCRRLCEWVKEAGTTTQ
                     NAPLVVDTALNGEAYKQQVARAVAEIRRGEYVKVIVSRAIPLPSRIDMPATLLYGRQA
                     NTPVRSFMFRQEGREALGFSPELVMSVTGNKVVTEPLAGTRDRMGNPEHNKAKEAELL
                     HDSKEVLEHILSVKEAIAELEAVCQPGSVVVEDLMSVRQRGSVQHLGSGVSGQLAENK
                     DAWDAFTVLFPSITASGIPKNAALNAIMQIEKTPRELYSGAILLLDDTRFDAALVLRS
                     VFQDSQRCWIQAGAGIIAQSTPERELTETREKLASIAPYLMV"
     gene            complement(4569..5849)
                     /gene="ybtX"
                     /locus_tag="NBI74_002099"
     CDS             complement(4569..5849)
                     /gene="ybtX"
                     /locus_tag="NBI74_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="yersiniabactin-associated zinc MFS transporter
                     YbtX"
                     /protein_id="HCD1932439.1"
                     /translation="MSDVQSNVKPLTLTTGRVIFAIAGVYVTQSLVSALSMQSLPALV
                     RAAGGSLALAGATTLFMLPWVLKFIWAPWIERWRLPPGSQERRSRMLILRGQVALAAI
                     LTIAAAIGWFGREGGFPDTQIVALFVLFMVAGTVASTIDIASDGFCVDQLTRTGYGWG
                     NSVQVGGSYLGMMCGGGVFLMLSAASGWPVAMLMMAVLIMALSLPLWRITEPTRTATI
                     PHVPALGYALRRKQARLGLLLVLMLNSGMRFVLPLLAPLLLDHGLSMSALGALFSGGN
                     IAAGIAGTLAGGLLMKYTSPGRALLTAYGVQGIALLAVVMTLMMAPGHLLLQILQCLV
                     IVQSISLACALVCLYATLMSLSSPLQAGVDFTLFQCTDAAIAILAGVIGGVVAQHFGY
                     AACFLFAGAFTLLAAWVAYIRLHSARELMTSAID"
     gene            complement(5842..7644)
                     /gene="ybtQ"
                     /locus_tag="NBI74_002100"
     CDS             complement(5842..7644)
                     /gene="ybtQ"
                     /locus_tag="NBI74_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="yersiniabactin ABC transporter
                     ATP-binding/permease protein YbtQ"
                     /protein_id="HCD1932440.1"
                     /translation="MKDNNPADNLAWRVIWRQLISSVGSQARMLRRSMLALLLAAFMQ
                     GIAFACLYPIIDTLLRGDAPQLLNWAMAFSVAAIVTLVLRWYGLGFEYRGHLAQATHE
                     LRLRLGEQLRRVPLEKLQRGRAGEMNALLLGSVDENLNYVIAIANILLLTIVTPLTAS
                     LATLWIDWRLGLVMLLIFPLLVPFYYWRRPAMRRQMQTLGEAHQRLSGDIVEFAQGMM
                     VLRTCGSDADKSRALLAHFNALENLQTRTHRQGAGATMLIASVVELGLQVVVLSGIVW
                     VVTGTLNLAFLIAAVAMIMRFAEPMAMFISYTSVVELIASALQRIEQFMAIAPLPVAE
                     QSEMPERYDIRFDNVSYRYEEGDGHALNHVSLTFPAASMSALVGASGAGKTTVTKLLM
                     RYADPQQGQISIGGVDIRRLTPEQLNSLISVVFQDVWLFDDTLLANIRIARPQATRQE
                     VEEAARAAQCLEFISRLPQGWLTPMGEMGGQLSGGERQRISIARALLKNAPVVILDEP
                     TAALDIESELAVQKAIDNLVHNRTVIIIAHRLSTIAGAGNILVMEEGQVVEQGTHAQL
                     LSHHGRYQALWQAQMAARVWRDDGGAASGEWVHE"
     gene            complement(7631..9343)
                     /gene="ybtP"
                     /locus_tag="NBI74_002101"
     CDS             complement(7631..9343)
                     /gene="ybtP"
                     /locus_tag="NBI74_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="yersiniabactin ABC transporter
                     ATP-binding/permease protein YbtP"
                     /protein_id="HCD1932441.1"
                     /translation="MALAGLAALTSLGALLFLAWSLRDIRATPDAIPAWPLGGVIGCV
                     VLTFVLRLQAFNTSHYAAFHLENILRSRLARKALQLPPGVLQQMGSGSVAKVMLDDVK
                     SLHIFVADSTPLYARAIIMPLATIVILFWLDWRLAIATLGVLAFGSVVLVLARQRSED
                     MAQRYHKAREQVSAAVIEFVQAMPVVRTFDSGSTSFLRYQRALEEWVDVLKTWYRKAG
                     FSARFSFSILNPLPTLFVLIWSGYGLLHYGSFDFIAWVAVLLLGSGMAEAVMPMMMLN
                     NLVAQTRLSIQRIYQVLAMPELSLPQSDQQPQEASITFEQVSFHYPQARTGAALQEVS
                     FHVPAGQIVALVGPSGAGKSTVARLLLRYADPDKGHIRIGGVDLRDMQTDTLMKQLSF
                     VFQDNFLFADTIANNVRLGAPDTPLEAVIAAARVAQAHDFISALPEGYNTRVGERGVF
                     LSGGQRQRITIARALLQDRPILVLDEATAFADPENEAALIKALAAAMRGRTVIMVAHR
                     LSMVTQADVILLFSDGQLREMGNHTQLLAQGGLYQRLWQHYQQAQHWVPGGTQEEVME
                     NERQ"
     gene            9600..10559
                     /gene="ybtA"
                     /locus_tag="NBI74_002102"
     CDS             9600..10559
                     /gene="ybtA"
                     /locus_tag="NBI74_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="yersiniabactin transcriptional regulator YbtA"
                     /protein_id="HCD1932442.1"
                     /translation="MTESPQTQSEISIHQLVVGKPANDGNIPAQCELLRCSLQEGMDI
                     LLWRGHFARPETLQLHDDLGRINFSCILEGTSRFAIQGLRRHTDWELARNRHYITHTP
                     DCRGSASYCGRFESITLSFSPETLALWVPDISAVIKNKIDSHCCCQQHRCNAETHLTA
                     QALRHALMRMHGGFSHEQKPSTLWLQGQSLVMLSLVLDEHREDASCLSCHFNPMERQK
                     LLRAKDLLLADLTQAPGVAELARESGLSVLKIKRGFRVLFNNSVYGLFQAERMQEARR
                     RLANGNTSVMTVAADLGYANASHFSAAFQKQFGVTPSTFKRGM"
     gene            10750..16857
                     /gene="irp2"
                     /locus_tag="NBI74_002103"
     CDS             10750..16857
                     /gene="irp2"
                     /locus_tag="NBI74_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="yersiniabactin non-ribosomal peptide synthetase
                     HMWP2"
                     /protein_id="HCD1932443.1"
                     /translation="MISGAPSQDSLLPDNRHAADYQQLRERLIQELNLTPQQLHEESN
                     LIQAGLDSIRLMRWLHWFRKNGYRLTLRELYAAPTLAAWNQLMLSRSPENAEEETPPD
                     ESSWPNMTESTPFPLTPVQHAYLTGRMPGQTLGGVGCHLYQEFEGHCLTASQLEQAIT
                     TLLQRHPMLHIAFRPDGQQVWLPQPYWNGVTVHDLRHNDAESRQAYLDALRQRLSHRL
                     LRVEIGETFDFQLTLLPDNRHRLHVNIDLLIMDASSFTLFFDELNALLAGESLPAIDT
                     RYDFRSYLLHQQKINQPLRDDARAYWLAKASTLPPAPVLPLACEPATLREVRNTRRRM
                     IVPATRWHAFSNRAGEYGVTPTMALATCFSAVLARWGGLTRLLLNITLFDRQPLHPAV
                     GAMLADFTNILLLDTACDGDTVSNLARKNQLTFTEDWEHRHWSGVELLRELKRQQRYP
                     HGAPVVFTSNLGRSLYSSRAESPLGEPEWGISQTPQVWIDHLAFEHHGEVWLQWDSND
                     ALFPPALVETLFDAYCQLINQLCDDESAWQKPFADMMPASQRAIRERVNATGAPIPEG
                     LLHEGIFRIALQQPQALAVTDMRYQWNYHELTDYARRCAGRLIECGVQPGDNVAITMS
                     KGAGQLVAVLAVLLAGAVYVPVSLDQPAARREKIYADASVRLVLICQHDASAGSDDIP
                     VLAWQQAIEAEPIANPVVRAPTQPAYIIYTSGSTGTPKGVVISHRGALNTCCDINTRY
                     QVGPHDRVLALSALHFDLSVYDIFGVLRAGGALVMVMENQRRDPHAWCELIQRHQVTL
                     WNSVPALFDMLLTWCEGFADATPENLRAVMLSGDWIGLDLPARYRAFRPQGQFIAMGG
                     ATEASIWSNACEIHDVPAHWRSIPYGFPLTNQRYRVVDEQGRDCPDWVPGELWIGGIG
                     VAEGYFNDPLRSEQQFLTLPDERWYRTGDLGCYWPDGTIEFLGRRDKQVKVGGYRIEL
                     GEIESALSQLAGVKQATVLAIGEKEKTLAAYVVPQGEAFCVTDHRNPALPQAWHTLAG
                     TLPCCAISPEISAEQVADFLQHRLLKLKPGHTAGADPLPLMNSLAIQPRWQAVVERWL
                     AFLVTQRRLKPAAEGYQVCAGEEREDEHPHFSGHDLTLSQILRGARNELSLLNDAQWS
                     PESLAFNHPASAPYIQELATICQQLAQRLQRPVRLLEVGTRTGRAAESLLAQLNAGQI
                     EYVGLEQSQEMLLSARQRLAPWPGARLSLWNADTLAAHAHSADIIWLNNALHRLLPED
                     PGLLATLQQLAVPGALLYVMEFRQLTPSALLSTLLLTNGQPEALLHNSADWAALFSAA
                     AFNCQHGDEVAGLQRFLVQCPDRQVRRDPRQLQAALAGRLPGWMVPQRIVFLDALPLT
                     ANGKIDYQALKHRHTPEAENPAEADLPQGDIEKQVAALWQQLLSTGNVTRETDFFQQG
                     GDSLLATRLTGQLHQAGYEAQLSDLFNHPRLADFAATLRKTDVPVEQPFVHSPEDRYQ
                     PFALTDVQQAYLVGRQPGFALGGVGSHFFVEFEIADLDLTRLETVWNRLIARHDMLRA
                     VVRDGQQQVLEQTPRWVIPAHILHTPEEALQVREKLAHQVLNPEVWPVFDLQVGYVDG
                     MPARLWLCLDNLLLDGLSMQILLAELEHGYRYPQQLLPPLPVTFRDYLQQPSLQSPNP
                     DSLAWWQAQLDDIPPAPALPLRCLPQEVETPRFARLNGALDSTRWHRLKKRAADAHLT
                     PSAVLLSVWSTVLSAWSAQPEFTLNLTLFDRRPLHPQINQILGDFTSLMLLSWHPGES
                     WLHSAQSLQQRLSQNLNHRDVSAIRVMRQLAQRQNVPAVPMPVVFTSALGFEQDNFLA
                     RRNLLKPVWGISQTPQVWLDHQIYESEGELRFNWDFVAALFPAGQVERQFEQYCALLN
                     RMAEDESGWQLPLAALVPPVKHAGQCAERSPRVCPEHSQPHIAADESTVSLICDAFRE
                     VVGESVTPAENFFEAGATSLNLVQLHVLLQRHEFSTLTLLDLFTHPSPAALADYLAGV
                     ATVEKTKRPRPVRRRQRRI"
     gene            16945..26436
                     /gene="irp1"
                     /locus_tag="NBI74_002104"
     CDS             16945..26436
                     /gene="irp1"
                     /locus_tag="NBI74_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="yersiniabactin polyketide synthase HMWP1"
                     /protein_id="HCD1932444.1"
                     /translation="MDNLRFSSAPTADSIDASIVQHYPDCEPVAVIGYACHFPESPDG
                     ETFWQNLLEGRECSRRFTREELLAVGLDAAIIDDPHYVNIGTVLDNADCFDATLFGYS
                     RQEAESMDPQQRLFLQAVWHALEHAGYAPGAVPHKTGVFASSRMSTYPGREALNVTEV
                     AQVKGLQSLMGNDKDYIATRAAYKLNLHGPALSVQTACSSSLVAVHLACESLRAGESD
                     MAVAGGVALSFPQQAGYRYQPGMIFSPDGHCRPFDASAEGTWAGNGLGCVVLRRLRDA
                     LLSGDPIISVILSSAVNNDGNRKVGYTAPSVAGQQAVIEEALMLAAIDDRQVGYIETH
                     GTGTPLGDAIEIEALRNVYAPRPQDQRCALGSVKSNMGHLDTAAGIAGLLKTVLAVSR
                     GQIPPLLNFHTPNPALKLEESPFTIPVSAQAWQDEMRYAGVSSFGIGGTNCHMIVASL
                     PDALNARLPNTDSGRKSTALLLSAASDSALRRLATDYAGALRENADASSLAFTALHAR
                     RLDLPFRLAAPLNRETAEALSAWAGEKSGALVYSGHGASGKQVWLFTGQGSHWRTMGQ
                     TMYHHSTAFADTLDRCFSACSEMLTPSLREAMFNPDSAQLDNMAWAQPAIVAFEIAMA
                     AHWRAEGLKPDFAIGHSVGEFAAAVVCGHYTIEQVMPLVCRRGALMQQCASGAMVAVF
                     ADEDTLMPLARQFELDLAANNGTQHTVFSGPEARLAVFCATLSQHDINYRRLSVTGAA
                     HSALLEPILDRFQDACAGLHAEPGQIPIISTLTADVIDESTLNQADYWRRHMRQPVRF
                     IQSIQVAHQLGARVFLEMGPDAQLVACGQREYRDNAYWIASARRNKEASDVLNQALLQ
                     LYAAGVALPWADLLAGDGQRIAAPCYPFDTERYWKERVSPACEPADAALSAGLEVASR
                     AATALDLPRLEALKQCATRLHAIYVDQLVQRCTGYAIENGVDAMTIMHRGRLLPRYQQ
                     LLQRLLNNCVVDGDYRCTDGRYVRARPIEHQQRESLLTELAGYCEGFQAIPDTIARAG
                     DRLYEMMSGAEEPVAIIFPQSASDGVEVLYQEFSFGRYFNQIAAGVLRGIVQTRQPRQ
                     PLRILEVGGGTGGTTAWLLPELNGVPALEYHFTDISALFTRRAQQKFADYDFVKYSEL
                     DLEKEAQSQGFQAQSYDLIVAANVIHATRHIGRTLDNLRPLLKPGGRLLMREITQPMR
                     LFDFVFGPLVLPLQDLDAREGELFLTTAQWQQQCRHAGFSKVAWLPQDGSPNAGMSEH
                     IILATLPGQAVSAVTFTAPSEPVLGQALTDNGDYLADWSDCAGQPEQFNARWQEAWRL
                     LSQRHGDALPVEPPPVAAPEWLGKVRLSWQNEAFSRGQMRVEARHPAGEWLPLSPAAP
                     LPAPQTHYQWRWTPLNVASIDHPLTFSFSAGTLARSDELAQYGIIHDPHASSRLMIVE
                     ESEDTLALAEKVIAALTASAAGLIVVTRRAWRVEENEALSASHHALWALLRVAANEQP
                     ERLLAAIDLAENTPWETLHQGLSAVSLSQRWLAARGDTLWLPSLSPNTGCAAELPANV
                     FTGDSRWHLVTGAFGGLGRLAVNWLREKGARRIALLAPRVDESWLRDVEGGQTRVCRC
                     DVGDAGQLATVLDDLAANGGIAGAIHAAGVLADAPLQELDDHQLAAVFAVKAQAASQL
                     LQTLRNHDGRYLILYSSAAATLGAPGQSAHALACGYLDGLAQQFSTLDAPKTLSVAWG
                     AWGESGRAATPEMLATLASRGMGALSDAEGCWHLEQAVMRGAPWRLAMRVFTDKMPPL
                     QQALFNISATEKAATPVIPPADDNAFNGSLSDETAVMAWLKKRIAVQLRLSDPASLHP
                     NQDLLQLGMDSLLFLELSSDIQHYLGVRINAERAWQDLSPHGLTQLICSKPEATPAAS
                     QPEVLRHDADERYAPFPLTPIQHAYWLGRTHLIGYGGVACHVLFEWDKRHDEFDLAIL
                     EKAWNQLIARHDMLRMVVDADGQQRILATTPEYHIPRDDLRALSPEEQRIALEKRRHE
                     LSYRVLPADQWPLFELVVSEIDDCHYRLHMNLDLLQFDVQSFKVMMDDLAQVWRGETL
                     APLAITFRDYVMAEQARRQTSAWHDAWDYWQEKLPQLPLAPELPVVETPPETPHFTTF
                     KSTIGKTEWQAVKQRWQQQGVTPSAALLTLFAATLERWSRTTTFTLNLTFFNRQPIHP
                     QINQLIGDFTSVTLVDFNFSAPVTLQEQMQQTQQRLWQNMAHSEMNGVEVIRELGRLR
                     GSQRQPLMPVVFTSMLGMTLEGMTIDQAMSHLFGEPCYVFTQTPQVWLDHQVMESDGE
                     LMFSWYCMDNVLEPGAAEAMFNDYCAILQAVIAAPESLKTLASGIARHIPRRRWPLNA
                     QADYDLRDIEQATLEYPGIRQARAEITEQGALTLDIVMADDPSPSAAMPDEHELTQLA
                     LPLPEQAQLDELEATWRWLEARALQGIAATLNRHGLFTTPEIAHRFSAIVQALSAQAS
                     HQRLLRQWLQCLTEREWLIREGESWRCRIPLSEIPEPQEACPQSQWSQALAQYLETCI
                     ARHDALFSGQCSPLELLFNEQHRVTDALYRDNPASACLNRYTAQIAALCSAERILEVG
                     AGTAATTAPVLKATRNTRQSYHFTDVSAQFLNDARARFHDESQVSYALFDINQPLDFT
                     AHPEAGYDLIVAVNVLHDASHVVQTLRRLKLLLKAGGRLLIVEATERNSVFQLASVGF
                     IEGLSGYRDFRRRDEKPMLTRSAWQEVLVQAGFANELAWPAQESSPLRQHLLVARSPG
                     VNRPDKKAVSRYLQQRFGTGLPILQIRQREALFTPLHAPSDAPTEPAKPTPVAGGNPA
                     LEKQVAELWQSLLSRPVARHHDFFELGGDSLMATRMVAQLNRRGIARANLQDLFSHST
                     LSDFCAHLQAATSGEDNPIPLCQGDGEETLFVFHASDGDISAWLPLASALNRRVFGLQ
                     AKSPQRFATLDQMIDEYVGCIRRQQPHGPYVLAGWSYGAFLAAGAAQRLYAKGEQVRM
                     VLIDPVCRQDFCCENRAALLRLLAEGQTPLALPEHFDQQTPDSQLADFISLAKTAGMV
                     SQNLTLQAAETWLDNIAHLLRLLTEHTPGESVPVPCLMVYAAGRPAHWTPAETEWQGW
                     INNADDAVIEASHWQIMMEAPHVQACAQHITRWLCATSTQPENTL"
     gene            26433..27533
                     /gene="ybtU"
                     /locus_tag="NBI74_002105"
     CDS             26433..27533
                     /gene="ybtU"
                     /locus_tag="NBI74_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="yersiniabactin biosynthesis oxidoreductase YbtU"
                     /protein_id="HCD1932445.1"
                     /translation="MMPSASPKQRVLIVGAKFGEMYLNAFMQPPEGLELVGLLAQGSA
                     RSRELAHAFGIPLYTSPEQITRMPDIACIVVRSTVAGGTGTQLARHFLTRGVHVIQEH
                     PLHPDDISSLQTLAQEQGCCYWVNTFYPHTRAGRTWLRDAQQLRRCLAKTPQVVHATT
                     SRQLLYSTLDLLLLALGVDAAAVECDVVGSFSDFHCLRLFWPEGEACLLLQRYLDPDD
                     PDMHSLIMHRLLLGWPEGHLSLEASYGPVIWSSSLFVADHQENAHSLYRRPEILRDLP
                     GLTRSAAPLSWRDCCETVGPEGVSWLLHQLRSHLAGEHPPAACQSVHQIALSRLWQQI
                     LRKTGNAEIRRLTPPHHDRLAGFYNDDDKEAL"
     gene            27530..28333
                     /gene="ybtT"
                     /locus_tag="NBI74_002106"
     CDS             27530..28333
                     /gene="ybtT"
                     /locus_tag="NBI74_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="yersiniabactin biosynthesis thioesterase YbtT"
                     /protein_id="HCD1932446.1"
                     /translation="MTQSAMCIPLWPARNGNTAHLAMCPFAGGSSSAFRHWQAEQLAD
                     CALSLVTWPGRDRLRHLEPLRSITQLAALLANELEASVSPDTPLLLAGHSMGAQVAFE
                     TCRLLEQRGLAPQGLIISGCHAPHLHSERQLSHRDDADFIAELIDIGGCSPELRENQE
                     LMSLFLPLLRADFYATESYHYDSPDVCPPLRTPALLLCGSHDREASWQQVDAWRQWLS
                     HVTGPVVIDGDHFYPIQQARSFFTQIVRHFPHAFSAMTAWQKQPSTSER"
     gene            28337..29914
                     /gene="ybtE"
                     /locus_tag="NBI74_002107"
     CDS             28337..29914
                     /gene="ybtE"
                     /locus_tag="NBI74_002107"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="yersiniabactin biosynthesis salycil-AMP ligase
                     YbtE"
                     /protein_id="HCD1932447.1"
                     /translation="MNSSFESLIEQYPLPIAEQLRHWAARYASRIAVVDAKGSLTYSA
                     LDAQVDELAAGLSSLGLRSGEHVIVQLPNDNAFVTLLFALLRLGVIPVLAMPSQRALD
                     IDALIELAQPVAYVIHGENHAELARQMAHKHACLRHVLVAGETMSDDFTPLFSLHGER
                     QAWPQPDVSTTALLLLSGGTTGTPKLIPRRHADYSYNFSASAELCGISQQSVYLAVLP
                     VAHNFPLACPGILGTLACGGKVVLTDSASCDEVMPLIAQERVTHVALVPALAQLWVQA
                     REWEDSDLSSLRVIQAGGARLDPTLAEQVIATFDCTLQQVFGMAEGLLCFTRLDDPHA
                     TILHSQGRPLSPLDEIRIVDQDENDVAPGETGQLLTRGPYTISGYYRAPAHNTQAFTA
                     QGFYRTGDNVRLDEVGNLHVEGRIKEQINRAGEKIAAAEVESALLRLAEVQDCAVVAA
                     PDTLLGERICAFIIAQQVPTDYQQLRQQLTRMGLSAWKIPDQIEFLDHWPLTAVGKID
                     KKRLTALAVDRYRHSAQ"
     gene            30046..32067
                     /gene="fyuA"
                     /locus_tag="NBI74_002108"
     CDS             30046..32067
                     /gene="fyuA"
                     /locus_tag="NBI74_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore yersiniabactin receptor FyuA"
                     /protein_id="HCD1932448.1"
                     /translation="MKMTRLYPLALGGLLLPAIANAQTSQQDESTLVVTASKQSSRSA
                     SANNVSSTVVSAPELSDAGVTASDKLPRVLPGLNIENSGNMLFSTISLRGVSSAQDFY
                     NPAVTLYVDGVPQLSTNTIQALTDVQSVELLRGPQGTLYGKSAQGGIINIVTQQPDST
                     PRGYIEGGVSSRDSYRSKFNLSGPIQDGLLYGSVTLLRQVDDGDMINPATGSDDLGGT
                     RASIGNVKLRLAPDDQPWEMGFAASRECTRATQDAYVGWNDIKGRKLSISDGSPDPYM
                     RRCTDSQTLSGKYTTDDWVFNLISAWQQQHYSRTFPSGSLIVNMPQRWNQDVQELRAA
                     TLGDARTVDMVFGLYRQNTREKLNSAYDMPTMPYLSSTGYTTAETLAAYSDLTWHLTD
                     RFDIGGGVRFSHDKSSTQYHGSMLGNPFGDQGKSNDDQVLGQLSAGYMLTDDWRVYTR
                     VAQGYKPSGYNIVPTAGLDAKPFVAEKSINYELGTRYETADVTLQAATFYTHTKDMQL
                     YSGPVGMQTLSNAGKADATGVELEAKWRFAPGWSWDINGNVIRSEFTNDSELYHGNRV
                     PFVPRYGAGSSVNGVIDTRYGALMPRLAVNLVGPHYFDGDNQLRQGTYATLDSSLGWQ
                     ATERMNISVYVDNLFDRRYRTYGYMNGSSAVAQVNMGRTVGINTRIDFF"
BASE COUNT         6939 a         9658 c         8908 g         6700 t
ORIGIN      
        1 agcaagtcat ctgtcatggt aaagctaacc agaaatatag gttaattttg gtgtttaaac
       61 cttttttact tgttagtatg atacaaaggc tttcaaaaaa gctgggaaat tcaacaactc
      121 ttggaaatta tggactttgt cccgcggctt taatctgacg gccgcgttcc ttttttggct
      181 caattattga ccccttctct acgcaacttc agttcccgcc agcaactttg cggcagtttc
      241 ataggatcaa tgtctaaaag aataatggtg atcgataaga aaacgactga ataactgtag
      301 attttcgctc gaaaccttcc tgtcagctcc atagcgaatc aagtgctgaa tgtcacagta
      361 tcgaacagaa aacagtgacg atctaaccct tcaagaatat tctacgattg ttctgtttag
      421 gaaaagcaag gcgggaagtc gggagataag tcattgataa agtggcggag agagggggat
      481 ttgaaccccc ggtagagttg cccctactcc ggttttcgag accggtccgt tcagccgctc
      541 cggcatctct ccgttcagat ggttgccatg atgccaggaa atttggcatt ttaacagtcc
      601 ctgtccgtgc aattttgttc aagtgacgag tttgcgagca aaacgatgat taagtggccc
      661 tggaaagtac aagaatcagc acatcaaact gcccttccct ggcaggaagc actatcgatc
      721 ccccttttaa cgtgtctgac agaacaggaa caaagcaaat tggtcgctct tgccgaacgt
      781 tttttacagc aaaaacggct tgttccttta cagggctttg agctgaattc attaagaagc
      841 tgccggatag cacttctatt ttgcctgccc gttctggagt taggactgga atggctggat
      901 ggttttcatg aaatcttaat ttatcctgcg ccatttgtgg tcgatgatga atgggaagac
      961 gatatcggtc tggtgcataa ccaacgtatt gttcagtcag gtcagagctg gcagcaaggg
     1021 cctatcgttt tgaactggtt ggatatacaa gattcttttg atgcttctgg ttttaacctg
     1081 attattcatg aagtcgctca taagctggac acccgtaacg gcgatcgcgc cagcggagtt
     1141 ccctttattt cgttgcgtga ggttgctggc tgggaacacg atcttcatgc tgcaatgaac
     1201 aacattcagg aagaaatcga attagttggt gagaatgcgg cgagcattga tgcttatgct
     1261 gccagtgatc ctgctgaatg ttttgccgta ctttctgaat atttctttag cgccccagaa
     1321 ctttttgctc ctcgtttccc ttcattgtgg caacgtttct gtcaatttta tcaacaagat
     1381 cctttgcaga gactgcatca cgctaatgat acagactcgt tttcggcgac gaatgttcat
     1441 taattaacaa gtttgaaggt taattaacca attgaaatgg cttatgaaat ttagtgttga
     1501 cagacaaggt accgctaagt aatattcgcc ccgttcacac gattcctctg tagttcagtc
     1561 ggtagaacgg cggactgtta atccgtatgt cactggttcg agtccagtca gaggagccaa
     1621 ttttctgttt tcatgcatcc ttgcgaatct ttatctgatg ttgattcaac aggttagtgt
     1681 gaaaactctt ctcggtgcat tttgatttta ccctacgcat cgggaaaaat tggtggtcaa
     1741 atctggggtc aggttagttc gataatggag tgacccccat atatccctta ccgacgcaaa
     1801 aatccgcacc ctcaagcctt ctgataaacc ctttaaagtc tccgattctc acggtctgta
     1861 tctgcgggtc aagccgggtg gctcccgcca ctggtatctc aaataccgta ttagcggtaa
     1921 agaatcccgc attgcgctgg gtgcctatcc agccatctcc ctgtctgatg cgcgacagca
     1981 acgtgaaggt atccgtaaaa tactggcgct gaatatcaac ccggtacagc agcgggctgc
     2041 tgaacgtggc tcacgaacac cggagaaagt ttttaaaaac gtggcgctgg cgtggcataa
     2101 aagtaacagg aaatggtcgc agaacaccgc cgaccgtctg cttgccagcc tgaacaatca
     2161 catctttccg gtcatcggga acctacctgt atcagaactt aaaccccgtc atttcattga
     2221 cctgctgaaa gggatcgagg aaaaagatct gctggaggtt gcgtcccgca cacggcagca
     2281 cctgagtaac ataatgcgcc atgcggtcca tcaggagtta atcgatacga accctgcagc
     2341 aaaccttggc ggcgtgacca cacctcctgt cagacggcac tatcctgccc tgccgctgga
     2401 gcggctgcct gaactgcttg aacgtattgg ggcatatcat cagggccgtg aactgacccg
     2461 gcatgccgtt ctgctgatgc tgcatgtgtt cattcgctcc agtgaactgc gtttcgcccg
     2521 ctggtcagag attgatttca caaaccgagt ctggacgata cccgcgacgc gagaacccat
     2581 tattggcgtg cgttattccg gccgcggggc aaaaatgcga atgccgcata tcgtccccct
     2641 ctcagaacag tccatcgcca ttctgaaaca gattaaggat atcaccggta ataatgaact
     2701 gatcttcccc ggcgaccata acccgtataa gccaatgtgt gaaaacacgg tcaataaggc
     2761 actgcgggtg atgggttacg acacgaaaaa ggatatctgc ggtcacggct tccgggcaat
     2821 ggcatgcagt gcgctgatgg aatcgggttt atgggcaaag gacgcagtag aacgccagat
     2881 gagtcatcag gagcgcaata ccgtgcgcat ggcttatatt cataaggcag agcacctgga
     2941 agcccgcaaa acgatgatgc agtggtggtc ggattatctg gaagcatgcc gagaatctta
     3001 tgcaccgcct tatacaattg gtaaaaataa gtttatccca tagtatacta acgaatccca
     3061 cactctggca aattaccacc acctccacaa gaggtggttc tatatagact tttatcaact
     3121 tcatgacgaa aaacacctat tttatcccaa aagaagacaa tagagtcaca gaggaatcaa
     3181 agagcgactc aattgtcttt ggacagtctg gttgtgaggt tagaaaacag ttactcctac
     3241 accattaaat agggcgcaat gctcgctaat ttctcccggg tttctgtcag ttcgcgttcc
     3301 ggcgtagatt gcgcgatgat tcccgccccc gcctgtatcc agcagcgctg gctatcctga
     3361 aatacggaac gcagaactag cgccgcatca aagcgcgtat cgtccagcag caggattgcg
     3421 ccggaataaa gctctcgcgg cgtcttctca atttgcataa tcgcgttcag agcagcattt
     3481 ttagggatac ctgaggcggt aatcgacgga aacagcacgg taaacgcatc ccaggcatcc
     3541 ttgttttccg caagctgacc gctcacgcca gaccccagat gctgaacgct gccgcgctgg
     3601 cgaaccgaca ttaaatcttc aaccaccacg ctgcccggct ggcaaacggc ctccagttca
     3661 gcaatagctt ctttgacaga aaggatatgc tcaagcacct ctttactgtc gtgcagcagt
     3721 tctgcctctt tcgccttatt atgctccggg tttcccatgc gatcgcgggt gcccgcaagc
     3781 ggttcagtga ccactttatt gcccgtcact gacatcacca gttccgggct aaagcccagc
     3841 gcttcgcgtc cttcctgacg gaacataaac gagcgcacag gcgtgtttgc ctgccgcccg
     3901 tataacagcg tggcgggcat atcaatccgc gatggcaggg gaatggcgcg cgagacaatc
     3961 actttgacat actcgccacg gcggatctcc gcaacggcgc gcgcaacctg ttgtttatat
     4021 gcctcaccat tgagggcggt atccaccacc agtggtgcgt tctgcgtcgt tgtaccggcc
     4081 tcttttaccc actcgcacaa acgtcggcac ccgtcggcgg agtccgcata aacggtgaca
     4141 tttccctttt caaaaataag ttcttcacgg ggaacggtta acgtcagcag cggccactcc
     4201 ccggcgttaa aggcaatccc ccgcgcatgc gccgcaaagt tgaacccaac ctgtccatat
     4261 acccgacgtc ctttcacctg aggatgcgcc ataaaacgac gcgcgcagtc ggcaatggaa
     4321 tccaccgccc atttttgctc cccggcatca tcaataaatg tcgcctgcgt tccgtcggca
     4381 ttaatgtgca gccgtgcctg gcaccctttg cctaaatacc aacagggttg acgctcatag
     4441 acataacact cttcttctgc gaactggcgt aaaacgccag aaatcgtcgg tagccattgt
     4501 tcctctggta acgccagatg tagaaattca ctgattttca tcccttacct ctctgtgtta
     4561 ttcgcggatc aatcaattgc gcttgtcatc agttctcttg ccgaatgcag ccggatataa
     4621 gcaacccacg ccgccagcaa cgtgaatgcc ccggcaaaca ggaagcaggc tgcatagcca
     4681 aaatgttgag caacaacgcc accgataacg cctgccagaa tggcgattgc cgcatcagta
     4741 cattgaaaga gggtgaagtc gacaccggcc tgcaaaggcg atgaaagcga catcagcgtg
     4801 gcgtaaagac agaccagcgc gcaggctagc gaaatggact ggacaatgac cagacactgg
     4861 agaatctgca gcagcagatg acccggcgcc atcatgagcg tcatcaccac cgccagcagc
     4921 gcgatcccct ggacgccata agccgtcaac agcgctctgc cgggtgaggt gtatttcatc
     4981 agcaatccgc cggccagcgt tcctgctatg cccgctgcaa tattgccgcc gctgaacagc
     5041 gcgcccaatg cggacatgct caacccatga tccaacaaca gcggtgccag aagaggcagc
     5101 acaaaccgca tgcctgaatt cagcatcaat accagcagta agcccaggcg cgcctgcttc
     5161 ctccttagcg cataacctaa cgccggaaca tgcgggatag tcgctgttcg cgtcggctcc
     5221 gtaatgcgcc acagcgggag tgacagcgcc ataatcagca ccgccatcat cagcatggcg
     5281 acaggccagc cggatgccgc cgacagcatc aggaacaccc cgccgccgca catcattccc
     5341 agatagctgc cgccgacctg cacgctgttt ccccagccgt aacccgtgcg ggtcagttga
     5401 tcgacacaaa agccgtcgct ggcgatatca atggtggagg cgaccgtgcc tgccaccata
     5461 aacagaacaa ataacgcgac gatttgcgta tcggggaatc ccccttctcg cccaaaccag
     5521 ccaatcgctg cggcaatagt caggatcgcc gctagcgcga cctgaccacg caggattaac
     5581 atgcgggaac ggcgttcctg gctaccgggc ggaagccgcc agcgctcgat ccacggcgcc
     5641 caaataaact tcagcaccca gggcagcata aacagggttg tcgcaccggc aagcgccagc
     5701 gaaccgccag cagcgcgcac cagcgcgggt aaggactgca tagacagcgc cgataccaga
     5761 ctctgcgtca catagacgcc ggcaatagca aaaatcaccc gcccggtcgt caacgtcaga
     5821 ggtttcacat tcgactgaac atcactcatg cacccactct ccagacgcgg cccccccgtc
     5881 gtcgcgccac acgcgcgcgg ccatttgcgc ctgccacagc gcctgataac gtccatgatg
     5941 tgagagcaat tgcgcatgag taccctgctc aaccacctgt ccctcttcca tcaccagaat
     6001 gtttccggcc ccggcgatgg tggataaacg gtgagcgatg ataatcaccg tccggttgtg
     6061 aaccaggtta tcgatcgctt tttgcaccgc cagctcgctt tcaatatcca gcgcggcagt
     6121 cggttcatcg agaatgacga ccggcgcgtt tttcaataac gctctggcaa tggaaatccg
     6181 ctggcgctcg ccgcccgata gctggccgcc catctctccc attggcgtca gccagccttg
     6241 cggaagacgg gaaataaact caaggcactg cgccgcgcgg gcggcctctt ctacctcctg
     6301 ccgcgtcgcc tgtgggcgcg cgatacggat attcgccagc agcgtgtcat caaacagcca
     6361 gacatcctgg aaaacaacag aaattaggct attgagctgt tccggcgtca ggcggcgaat
     6421 atcgacgccg ccaatagaaa tctgcccttg ctgcggatcg gcatagcgca ttaacagttt
     6481 ggtgacggta gttttgcctg cgccggaggc acccaccagc gcgctcatac tggctgccgg
     6541 gaacgtcaaa gaaacatgat taagcgcgtg gccgtcgcct tcttcgtagc gatagctgac
     6601 gttgtcaaag cggatatcgt aacgttccgg catctcgctt tgctctgcga cgggaagcgg
     6661 tgctatcgcc ataaactgct caatccgttg cagggcgctg gcgatcagtt ccacaaccga
     6721 ggtgtagctg ataaacatcg ccatcggttc tgcgaagcgc ataatcatcg cgacggcggc
     6781 aatcaaaaag gcgaggttca gagtgcccgt cactacccag acgatcccgg ataacaccac
     6841 cacctgtagg cccaactcca cgacgctggc gatcagcatc gtcgcgccag cgccctgacg
     6901 gtgagtgcgg gtctgtaagt tttccagcgc attgaaatgc gccagcagcg cccggctttt
     6961 atcggcatcg ctgccgcagg tgcgcaatac catcatcccc tgagcaaatt caacgatatc
     7021 gccgctcaga cgctggtgcg cttcccccag cgtctgcatt tgtcgccgca tcgccgggcg
     7081 gcgccagtaa taaaacggca ccagcagagg gaagatcagc aacatcacca gccccagccg
     7141 ccagtctatc cacaatgtcg ccagcgacgc cgtcagcggc gtgacaatgg tgagcagcaa
     7201 aatattggct atcgcaataa cataattgag gttttcatcc acgctgccca gcagcaaggc
     7261 gttcatttca cccgccctgc cgcgctggag cttctccagc ggcacgcggc gtaactgctc
     7321 gccaagtcgc aggcgcaact catgggtagc ctgcgccaga tgaccacggt attcaaagcc
     7381 caggccatac cagcgtagca ccagcgtcac aattgcggcg acgctgaagg ccatagccca
     7441 gttaagaagt tgcggcgcgt ctccccgtaa cagcgtatca atgatcggat aaagacaggc
     7501 aaaggcgatc ccctgcatga atgccgccag caacagcgcc agcatactgc gtcgcagcat
     7561 cctggcctga ctgccaacgc tggagataag ctggcgccag atgacgcgcc aggccaggtt
     7621 atccgcagga ttattgtctt tcattttcca tcacctcttc ctgtgttcca cccggcaccc
     7681 aatgctgcgc ctgctgatag tgttgccaga gccgttgata caggccgccc tgcgccaaca
     7741 gttgcgtatg gttccccatt tccctgagct gtccgtcgga aaacagcaat atcacatcgg
     7801 cctgagtcac cattgagaga cgatgcgcga ccatgatgac cgtccggcca cgcatggccg
     7861 ccgcgagcgc cttgataagc gccgcttcgt tttccgggtc agcaaacgcc gtcgcctcat
     7921 cgagcaccag gatggggcga tcctgcaaaa gcgcccgggc gatagtaatg cgctgccgct
     7981 ggccgccgga gagaaatacc ccacgttccc cgactcgagt gttgtaacct tctggcagag
     8041 cgctaataaa atcatgggcc tgcgccactc tggccgccgc tataaccgcc tccagcggcg
     8101 tatccggcgc gcccagacga acgttattgg ctatcgtgtc ggcaaaaagg aagttgtcct
     8161 gaaacacaaa cgagagttgc ttcatcaggg tgtccgtctg catatcacgc agatccacgc
     8221 cgccaatacg gatatggcct ttgtccgggt cggcgtaacg cagcagcaag cgcgccacgg
     8281 tgctttttcc ggcgccgctt ggcccgacca gcgccacaat ttgcccggca ggcacatgaa
     8341 agctcacctc ctgcaacgcg gcgccagtac gcgcttgcgg ataatgaaag ctcacctgct
     8401 caaaggtaat gctcgcctct tgcggctgct ggtcagactg cggcagcgat aactccggca
     8461 tcgcgagaac ctgataaata cgctgaatgc ttaaacgcgt ttgcgcgacc aggttattga
     8521 gcatcatcat tggcattacg gcttcggcca ttccgctgcc cagcagtaaa acggccaccc
     8581 acgcgataaa atcgaaactg ccatagtgca acaggccgta tcccgaccag atcaggacaa
     8641 acagggtcgg gagaggattc agaatcgaga aggaaaaacg cgctgaaaaa ccggctttgc
     8701 gataccaggt tttgagcaca tcgacccact cttcaagggc gcgttgatag cgcaaaaaac
     8761 tggtgctgcc gctatcaaac gttctcacca cgggcatggc ctgcacgaac tcaataaccg
     8821 ccgcgctaac ctgctcgcgc gccttatgat aacgctgagc catatcttcc gaacgctggc
     8881 gggcgagcac gagaacaacc gatccaaacg ccagtacccc cagcgtcgcg attgccagcc
     8941 gccagtccag ccagaacaag ataacgattg tcgccagcgg catgatgatc gcgcgggcat
     9001 agagcggcgt gctgtcggcc acaaaaatat gtaacgactt cacgtcatcc agcatcactt
     9061 tcgccactga cccgctgccc atttgctgta acacgccagg cggaagctgc aatgctttac
     9121 gggctaacct gctgcgcaga atgttctcca gatgaaaagc cgcgtaatga gaggtgttga
     9181 acgcctgtaa gcgcagaaca aaagttaaga ccacgcagcc gatcacgccg cccagcggcc
     9241 aggcaggaat agcgtcaggc gtcgcgcgaa tgtcacgcag gctccacgcc agaaaaagga
     9301 gcgcccccag gctggttaat gccgccagcc ccgccagcgc catagctaaa ataaccttcc
     9361 tgcgaaccgg tgtaatcacc tgccaaagag gaaatcggga cagcgattca gtattggatg
     9421 attgagatga catgggagta actgaatttc ctgatgaatt tttacggacg ccaaaaacga
     9481 caattcaggg tggcgttctg agaattaatg tgaataataa ccattatcaa taactcatct
     9541 accccattcg gttctattta tacccgcatt ggtctaagcc acagggagat aaccaggtca
     9601 tgacggagtc accgcaaacg caatctgaaa tctctattca ccagttggtg gtcgggaaac
     9661 ctgcaaacga tggcaatatt cctgctcagt gtgaactgct gcgctgttcg ttgcaggaag
     9721 gtatggatat tttgctctgg aggggccatt ttgcgcggcc tgagacgttg cagctacacg
     9781 acgatctggg caggattaac ttctcgtgca tcctggaagg cacatcgcgc tttgcgattc
     9841 agggactccg gcgtcacacc gactgggagc ttgcgcgcaa tcgccattac attacccata
     9901 cgccagactg tcgcggcagc gcctcctatt gtggccgatt cgagagcatt accctctcct
     9961 tcagccctga aaccctcgcg ctgtgggtgc cggatattag cgcggtcatt aagaataaga
    10021 tcgactccca ctgctgttgc cagcagcacc gttgtaatgc tgaaacccac ttaacggctc
    10081 aggccttgcg tcacgcgctg atgcgcatgc acggcggctt cagtcatgaa caaaagccgt
    10141 cgacgctatg gttgcagggg caaagtctgg tcatgctcag tctggtgctg gatgaacatc
    10201 gcgaagacgc cagttgcctc tcctgccact tcaacccgat ggaacgccag aaactgctgc
    10261 gggcgaagga tctgctgctg gctgatctga cgcaagcgcc gggcgtcgcg gagctggcca
    10321 gagagtccgg tctgagcgtt ctcaaaatta agcgaggctt ccgcgttctg ttcaacaaca
    10381 gcgtctatgg cctgtttcag gctgaacgaa tgcaggaagc cagaagacgc ctggccaacg
    10441 gcaatacctc cgtcatgacg gtggcggccg atctgggcta tgccaacgcc agccatttct
    10501 ctgccgcctt ccagaaacag tttggcgtaa ctccttcgac ctttaaacgc gggatgtaac
    10561 cggggtcgcg cccccctaaa ctcctccctg acagaggccc ggatacccca tccgggcctc
    10621 tgttctttcc gcctgttacc aacccgaaac gggcataaat aaacccgttt cgggtagcat
    10681 ccaattgtta ataattatta ttctcatatg agtaatgctt ttcggtaaga cgtgccatca
    10741 ggaggaagaa tgatttctgg cgcaccatct caggattcgc tgttaccgga caaccgccac
    10801 gcggctgatt accaacaatt acgcgagcgg ctcatacagg aactgaattt aacgccgcag
    10861 cagttacatg aagagagcaa cctgatccag gccggcctgg attccataag attgatgaga
    10921 tggttacact ggtttcgtaa aaatggctac cgccttaccc ttcgcgagct gtatgccgcc
    10981 cccacgctgg cggcatggaa ccagttaatg ctcagccggt cgccggagaa cgcggaagaa
    11041 gaaacgccgc ccgacgaatc atcctggccg aacatgaccg aaagtacccc cttcccattg
    11101 acgccagtac agcacgccta cctgacgggc cgcatgccgg ggcagacgct tggcggcgtg
    11161 ggttgccacc tgtatcagga gtttgaaggc cattgtctga cggcgtcgca gctggagcag
    11221 gccatcacga ccttgctgca acgccaccca atgctgcata tcgcctttcg ccccgacggg
    11281 cagcaggtct ggctaccgca accttactgg aacggcgtca ccgttcatga tttacgccat
    11341 aacgacgctg aaagccgcca ggcctatctg gacgcactgc gccagcgcct gagccaccgt
    11401 cttttacgcg tggaaatcgg cgaaacgttt gattttcagc tgacgctctt gccggacaat
    11461 cgccaccgcc tccatgtcaa tattgacctg ctgattatgg atgcctccag ctttacgctt
    11521 ttcttcgatg agcttaacgc cctgctggcc ggagaatcgc tgccggctat cgacacccgc
    11581 tatgatttcc gctcgtattt gctgcaccag cagaagatca atcaaccact gagagacgac
    11641 gcgcgcgctt actggctggc gaaagcatcg acgcttcccc ccgcgcccgt cttgccgctg
    11701 gcctgcgaac ccgccacgct acgtgaagtc cgtaataccc gacgccgcat gattgtcccg
    11761 gcaacacgct ggcacgcctt tagcaaccgg gccggcgagt atggcgtgac gccgacaatg
    11821 gcactggcga cctgtttttc tgccgtgctg gctcgctggg gcggcctgac gcgtctgctg
    11881 cttaacatca ccttattcga ccgccagccg ctgcacccgg cggttggcgc gatgcttgcc
    11941 gacttcacca atattcttct gctggatacc gcctgcgatg gcgataccgt cagcaacctg
    12001 gcgcgtaaaa accagctcac gtttacggag gactgggagc atcgccactg gtccggcgtc
    12061 gaattactcc gtgaactcaa acgccagcag cgctaccccc acggcgcccc ggtggtattt
    12121 accagcaatc tggggcgttc cctctacagc agccgcgcag aatcgccgtt gggcgagccg
    12181 gaatggggca tctcgcaaac gccgcaggtc tggatagatc atctggcgtt cgagcatcac
    12241 ggcgaggtct ggctacaatg ggacagcaac gacgcgctgt tccctccggc gttagtcgaa
    12301 acattgttcg acgcctactg ccagttgatt aaccaactct gcgatgacga aagcgcctgg
    12361 caaaagccgt tcgcagatat gatgcccgcc agccagcgcg cgatacgcga acgggtcaac
    12421 gccaccggcg cccccattcc cgaaggcttg ctgcatgaag gcattttccg tatcgctctg
    12481 caacagccgc aggcgctggc ggtaacggac atgcgttatc agtggaatta tcatgagctg
    12541 acagactatg cccgccgttg cgcgggcagg ttaatcgagt gcggggttca gcccggcgat
    12601 aatgtggcta tcacgatgtc gaaaggcgca ggacaacttg ttgcggttct ggccgtcctg
    12661 ctggctgggg cggtttacgt tccggtttcg ctggatcagc ctgccgcacg gcgcgagaaa
    12721 atctacgctg acgccagcgt ccggctggtg ctcatttgtc agcacgacgc cagcgccggg
    12781 tcagacgata ttcccgtcct tgcctggcag caggccattg aggcggagcc gatcgccaac
    12841 ccggtagtac gcgcccccac gcaaccggcc tacattatct acacctccgg ctctaccggt
    12901 acgccgaaag gggtagtcat ttctcaccgg ggagcgctta acacctgttg cgatatcaat
    12961 acccgctatc aggttggccc gcatgacagg gtgctggccc tctccgccct acattttgat
    13021 ttatcggttt acgacatttt tggcgtactg cgcgcgggcg gcgcgctggt gatggtgatg
    13081 gaaaatcaac ggcgcgatcc tcacgcatgg tgtgagctga tccagcgcca tcaggtcacg
    13141 ctctggaaca gcgtcccggc gctgttcgat atgctgctga cctggtgtga aggtttcgcc
    13201 gacgccacgc cggaaaacct gcgcgcagtg atgctttccg gcgactggat cgggcttgac
    13261 ctccccgccc gttatcgggc cttccggcca caaggacaat ttatcgcgat gggcggtgcc
    13321 accgaggcgt ctatctggtc taacgcctgc gaaattcacg acgtccccgc ccactggcgc
    13381 tccatccctt acggttttcc gctaaccaac caacgctacc gggtggtgga tgaacagggc
    13441 cgggactgcc ctgactgggt gccgggtgaa ttatggattg gcggcattgg ggtcgcggaa
    13501 ggctatttca acgatcccct gcgtagcgag cagcaatttt tgacgctccc ggacgagcgc
    13561 tggtatcgca ccggcgatct cggctgctac tggccagatg gcacaatcga gttcctcggt
    13621 cgtcgcgaca agcaggtcaa agtcggagga tatcgcatcg agctgggcga aatcgaaagc
    13681 gcgctcagcc agctggcggg ggtgaaacaa gcaaccgttc tggcgatcgg cgaaaaagaa
    13741 aaaacgctgg cggcatacgt tgttcctcag ggcgaggctt tttgcgttac cgatcatcgg
    13801 aacccggcac tgccgcaggc gtggcacacg cttgcgggaa cgttgccctg ttgcgccatc
    13861 tcgccagaga tctccgcaga acaggtagcc gatttccttc agcatcgcct gctaaaactg
    13921 aagccgggtc acaccgctgg cgccgatcct ctccccctga tgaactcact cgctatccag
    13981 ccgcgctggc aggccgtggt ggaacgctgg ttagcatttc tggtgacaca acggcgactg
    14041 aagcccgctg ctgaaggtta tcaggtctgc gctggtgaag aacgcgagga tgagcacccg
    14101 cacttcagcg gacatgattt aacgttatcg caaattcttc gcggtgcccg taacgaactg
    14161 tcgttactga acgacgcgca gtggtcgccg gaaagcctgg cctttaacca tccggccagc
    14221 gccccgtata ttcaggaact ggcgacaatt tgccaacagc ttgcacagcg cttacagcgc
    14281 ccggtgcgcc tgcttgaggt gggaacccgc actggccgcg ccgcagaatc gctgttagca
    14341 cagctcaacg ccggacagat tgagtatgtc gggcttgagc agagccagga gatgctgctg
    14401 agcgcccggc agaggctcgc cccctggcct ggcgcccgtc tgtccctctg gaatgcagac
    14461 acgctggcgg cgcacgctca ctcggcggac attatctggc ttaataacgc cctgcatcgt
    14521 ctgctgccgg aagatcccgg gctccttgcg acattacaac agcttgccgt tcccggcgcg
    14581 ctgctctacg tgatggagtt tcgccagtta acgccgtccg ccctactcag cacgctcctg
    14641 ttaaccaatg ggcagccgga ggccttgctg cataacagcg ccgactgggc ggcattattt
    14701 agcgcggccg ccttcaactg tcagcatggc gatgaggtcg cggggttaca acgcttcctc
    14761 gtacaatgtc ctgacaggca ggtgcgccgc gatccccgtc aacttcaggc cgccctcgcc
    14821 gggcgtctgc cggggtggat ggtgccgcaa cggatcgtct tcctcgacgc cttaccgctg
    14881 acggctaacg ggaaaattga ctaccaggcg ctgaagcatc gtcatacccc tgaagcggaa
    14941 aacccggccg aagcggattt accccagggc gacattgaaa aacaggttgc cgccctctgg
    15001 cagcaactct tatcaactgg caatgtcacc agagaaaccg acttcttcca gcaaggcggc
    15061 gatagcctgc tggcgacccg tctgaccggg caacttcatc aggcaggtta tgaagcgcaa
    15121 ttaagcgacc tgtttaatca tccccggctg gcggattttg ccgccacgct gcggaaaacc
    15181 gacgtcccgg tcgaacaacc attcgtccac tcccctgaag atcgctacca gccctttgcg
    15241 cttaccgacg tgcagcaggc ttacctggtg gggcgtcagc cgggctttgc cctgggcggc
    15301 gtcggctcac atttctttgt tgaatttgaa attgccgatc tggatctcac ccggttggag
    15361 acggtctgga accgattaat cgcccgccac gatatgctac gcgccgtcgt gcgtgatgga
    15421 cagcaacagg tgctcgaaca gacgcctcgc tgggtaatac ccgcacacat cctccatacg
    15481 cctgaagagg cgttgcaggt gcgcgaaaaa ctggcccatc aggtactcaa ccccgaagtc
    15541 tggccggtat tcgatctcca ggtcggttac gttgacggga tgcccgcccg cctgtggctg
    15601 tgtctggata acctgttact tgacgggctg agcatgcaga ttctgttggc ggagctggag
    15661 cacggctacc gctacccgca acagctgctt ccgccgctgc ccgtcacctt cagggattat
    15721 ctgcaacaac cctcgctaca gtcgcccaat ccagattctc tggcatggtg gcaggcgcag
    15781 cttgatgata ttcctccggc gcctgcgttg ccgctgcgct gcttgcctca ggaggttgaa
    15841 acaccgcgct tcgcccgcct gaacggcgca ctggacagca cgcgctggca tcggctgaaa
    15901 aaacgggcgg ctgacgccca tctcaccccg tcggccgtac tgttgtcggt gtggtcaacg
    15961 gttctctctg catggagtgc acagcctgag ttcacgctta accttacgct tttcgacagg
    16021 cgaccgctgc acccgcaaat caaccagatt ctgggcgatt tcacctcgct gatgctgctg
    16081 agctggcatc ccggcgaaag ctggctgcac agcgcgcagt cactacagca gcggctgagc
    16141 cagaacctca accaccgcga tgtgtcagcc atccgcgtga tgcgtcaact ggcgcaacgg
    16201 caaaacgtgc ctgccgttcc gatgcccgtc gtctttacca gcgcactggg ctttgagcag
    16261 gataacttcc tcgcccggcg taatctgctc aaaccggtct ggggcatctc ccagacgccg
    16321 caggtctggc tcgatcacca gatttatgaa tccgaaggcg aactgcgctt taactgggat
    16381 tttgtcgccg cgctgtttcc tgccgggcag gtggagcgcc agtttgaaca gtattgcgca
    16441 ttgctaaacc gaatggccga ggatgaaagc ggctggcaac tgccgctcgc cgcgctggtg
    16501 cctcccgtta aacacgcagg gcaatgcgca gagcgctcac cgcgcgtatg ccctgagcac
    16561 tctcagccac acattgcggc ggacgagagc accgtcagcc tgatttgcga cgccttccgc
    16621 gaggtggttg gcgagtctgt cacgcccgca gaaaacttct ttgaggcggg cgcaacgtcg
    16681 ctgaatctgg tgcaactgca cgttttgtta caacgtcacg aattttccac cctgacgttg
    16741 cttgacctct tcacccaccc ttctcctgct gccctggccg attatctggc cggcgtcgcc
    16801 acggtggaga aaacaaaacg acctcgccct gttcgccgtc gtcagcggcg gatatagcgc
    16861 gaagcaaact gattttcccc ggaacgccat cgcgaacgca tggcgttcca ttgactttgt
    16921 gaatcttagg aaacgggacc gattatggat aacttgcgct tctcttctgc gccgacagca
    16981 gattccattg atgcatcgat cgttcaacac tacccggact gcgaacctgt cgcggttatc
    17041 ggctacgcct gccattttcc tgaatcgccg gatggcgaaa cgttctggca aaatctgctg
    17101 gaaggtcgtg aatgcagccg acgctttacg cgcgaagagc ttctggccgt cggtctggat
    17161 gccgccatca ttgacgatcc tcattatgtc aatatcggta cggtgttaga caacgccgac
    17221 tgcttcgacg ccaccctgtt tggctattcg cgacaggaag cggagtcgat ggacccgcag
    17281 cagcgcctgt ttttgcaggc ggtctggcat gcgctggaac atgccggtta tgcccccggc
    17341 gccgtccccc ataagaccgg cgttttcgcc tcttcccgga tgagtaccta ccccggtcgc
    17401 gaagcattga acgtgacaga agtcgcgcag gtaaaaggtc tgcaatctct gatgggcaat
    17461 gataaagact atattgccac ccgcgccgcg tacaaactca acctgcacgg cccggcgtta
    17521 tcggtacaga ccgcctgctc cagctcgctg gttgccgtgc atctggcctg tgaaagcctg
    17581 cgcgcaggcg aatccgatat ggcggttgcc ggcggcgtgg cgctctcttt cccccagcag
    17641 gcaggctacc gctaccagcc cggaatgatt ttctctcctg atggtcactg tcgtcccttt
    17701 gacgcctcgg ctgagggcac ctgggccggt aacggtctcg gctgcgtggt gctgcgtcgc
    17761 ctgagagacg cgctgctgtc aggcgatccg attatctcgg tgatcctctc cagcgcggtc
    17821 aacaacgacg gcaacagaaa ggtcggctat accgcccctt ccgtcgcagg gcaacaggca
    17881 gtcatcgaag aggcgttaat gctggcggcc atcgacgaca ggcaggtagg ttacattgaa
    17941 acccacggca ccggcacacc gctgggcgac gcgattgaaa ttgaagcgtt acgcaacgtc
    18001 tatgcgcctc gcccgcagga tcagcgctgt gcgctcggtt ccgtgaaaag taacatgggc
    18061 catctggata ccgcggcggg cattgccgga ctgctgaaaa ccgttctggc agtcagtcgc
    18121 gggcaaattc ctcccttact gaattttcac acccccaacc cggcgctgaa acttgaagag
    18181 agccccttta ccataccggt gtcggcacag gcatggcagg acgaaatgcg ctatgcgggc
    18241 gtctcctcct ttggtattgg cggcaccaac tgccatatga tcgtcgcctc gctgcccgac
    18301 gcgctcaacg cgcgcctccc caatacggat agcggcagaa aaagtaccgc gctgctgctc
    18361 agcgccgcca gcgacagcgc gttgcggcgg ctggcgacgg attatgccgg ggcgctgaga
    18421 gagaatgcgg atgccagctc tctggccttc acagccctgc acgcgcgccg tctcgatctc
    18481 cccttccgcc tggcggcgcc attaaaccgt gaaaccgccg aggcgctcag cgcctgggcc
    18541 ggtgagaaat cgggggcgct ggtttacagc ggccacggcg ccagcggcaa gcaggtgtgg
    18601 ctgtttaccg gccagggctc gcactggcgc actatgggtc aaacgatgta ccatcactca
    18661 acggcgtttg ccgacacgct ggatcgctgt ttttccgcct gtagcgaaat gctcacgccg
    18721 tcactgcgcg aagcgatgtt taaccccgat tcggcgcagc tggacaatat ggcctgggca
    18781 cagccggcga ttgtcgcgtt tgaaatcgcg atggcggcgc actggcgtgc tgaaggactg
    18841 aagccagact tcgccattgg gcattccgtc ggtgaatttg ccgctgccgt tgtctgcgga
    18901 cactatacga ttgaacaggt catgccactg gtttgtcggc gcggcgcgct aatgcagcag
    18961 tgcgcaagcg gcgcgatggt ggcggtattt gcagacgaag acacgctgat gccgctggct
    19021 cgccagtttg agctggatct cgccgccaac aacggtacgc aacatacggt attttccggg
    19081 ccggaagccc gtctcgcggt attttgcgcc acgctctcgc agcatgacat taactatcgt
    19141 cgcctgagcg taaccggtgc ggcgcactcc gctttactgg agccgatact cgatcggttc
    19201 caggacgcct gcgcgggact gcacgcggag ccggggcaaa taccgattat ttccacgctc
    19261 accgccgacg tcattgatga gtcaacgctc aaccaggcgg attactggcg ccgacacatg
    19321 cgccagccgg tgcgttttat ccagagtatt caggtggcgc atcagctcgg cgcccgcgtt
    19381 tttctggaga tggggcccga tgcccagttg gttgcttgcg ggcagcgcga ataccgcgat
    19441 aacgcatact ggatagccag cgcccggcgt aacaaagagg cgagcgatgt cctcaatcag
    19501 gccctgctcc agctttacgc tgccggcgtc gccctaccgt gggccgacct gctggcgggc
    19561 gatggacaac gtatcgctgc gccatgttat ccgtttgata ctgaacgtta ctggaaagag
    19621 cgcgtctccc cggcctgcga gcctgccgac gcagcgctgt ctgccgggct ggaggtggcg
    19681 agtcgcgccg cgacagcgct cgatctccct cgcctggaag cgcttaaaca gtgcgccacg
    19741 cgactgcacg ccatctacgt cgatcaactg gtacaacgct gtaccggcta tgccattgag
    19801 aacggcgtgg acgccatgac catcatgcac cgtggacgtc tgctgccccg ctaccagcag
    19861 ctactccagc gcctgctgaa taactgcgtg gtcgacggcg attaccgctg caccgacggg
    19921 cgatacgtcc gcgcccgccc cattgaacat caacagcggg aatcactgct gacggaactt
    19981 gccggttatt gtgaaggttt tcaggctatt cccgacacca tcgcccgtgc cggcgatcgg
    20041 ttatatgaaa tgatgagcgg cgcggaagaa ccggtggcga ttatcttccc gcaaagcgcc
    20101 tccgacggcg tggaagtgct gtatcaggaa ttcagctttg gccgctattt caaccaaatc
    20161 gccgccgggg tattacgcgg cattgtccag acgcgtcagc cccgccagcc gttgcgtatt
    20221 cttgaagttg gcggcggaac cggcggcacc accgcgtggc tgctgccgga actcaacggc
    20281 gttccggcac tggagtacca tttcaccgat atctcggcgc tgttcacccg tcgcgcccag
    20341 cagaaattcg ccgactacga ttttgtgaag tatagcgagc tggatctcga aaaagaggcg
    20401 cagtctcagg gtttccaggc acagtcttac gatcttatcg tggcagcgaa cgtgattcac
    20461 gccacccgcc atattggccg cacgctcgat aatctgcgcc ccctgctcaa gccgggcggg
    20521 cgcctgctga tgcgcgaaat cacccagcca atgcgtctgt ttgacttcgt tttcggcccg
    20581 ctggttcttc cgctacagga tctcgacgcc cgcgaaggtg agttattcct caccaccgct
    20641 cagtggcaac aacagtgccg ccacgccgga ttcagcaaag tggcgtggct accgcaggat
    20701 ggcagcccga acgccgggat gagcgaacat atcattctcg ccacgctgcc cggtcaggcg
    20761 gttagcgccg taacattcac cgcgccatca gaacccgtgt tggggcaggc gctgacggat
    20821 aacggtgatt atctcgccga ctggtctgat tgcgcaggtc agcccgaaca gtttaacgct
    20881 cgctggcagg aggcatggcg tctgctttca cagcgtcatg gcgacgctct gcctgtggaa
    20941 ccgccccccg tcgccgcccc ggagtggctg gggaaggttc gcttaagctg gcaaaacgaa
    21001 gccttttccc gcggtcagat gcgcgttgaa gcccgtcatc ctgctggcga gtggctgccg
    21061 ctatcgcccg ccgcgcctct tcctgcgccg caaacgcatt atcaatggcg ctggacgccc
    21121 ctcaacgtcg ccagcattga ccatccgctt acctttagct tcagcgccgg tacgcttgcg
    21181 cgcagcgacg agctggcgca atacggcatc attcacgatc cgcacgcctc ttcacgactg
    21241 atgattgttg aggagagcga ggatacgctg gccttagcgg agaaagtgat agcagcgctc
    21301 accgccagcg cagccggatt gattgtggtt actcgccgcg cgtggcgagt cgaggaaaat
    21361 gaagcactct ctgcatccca tcacgcgcta tgggccttgc ttcgcgtcgc ggccaacgaa
    21421 cagccggaac ggttgcttgc cgccatcgat ctcgccgaaa acaccccgtg ggaaacgctg
    21481 catcaagggt tgagcgcagt ctcactatca cagcgctggc tcgccgcacg gggtgacacc
    21541 ctttggcttc cttcactgtc gcccaatacg ggatgcgccg ctgaattacc ggcaaacgtg
    21601 tttaccggcg atagccgctg gcatctggtg accggagcgt ttggcggatt aggccgcctt
    21661 gccgtgaact ggctcagaga aaaaggggcg cgacgcatcg ccctgctggc gccgcgcgtg
    21721 gatgagtcat ggctacgcga cgtggagggc gggcagacgc gcgtctgccg ttgtgatgtg
    21781 ggcgatgccg ggcaactggc cacggttctt gacgatctgg cggccaacgg cggcattgcc
    21841 ggagcgattc atgccgctgg cgtattggct gacgcgccct tgcaggagct tgatgaccac
    21901 cagctggctg ccgttttcgc ggtaaaagcg caggcggcaa gccagctgtt gcaaaccctg
    21961 cgcaaccacg acggacgcta tcttattctc tactcttccg ctgccgccac cctcggcgcg
    22021 ccgggtcaga gcgcccatgc gctggcctgc ggctacctgg acgggctggc ccagcagttt
    22081 tccacccttg atgcgccgaa aacgctctct gtcgcctggg gcgcatgggg agaaagcggt
    22141 cgggcggcca cgccggaaat gctggcgacg ctcgccagcc gaggtatggg cgcgttaagc
    22201 gatgccgaag gctgctggca cctggaacag gcggtgatgc gcggcgcccc gtggcgactg
    22261 gcgatgcgcg tttttaccga caaaatgccc ccgttacaac aggctctgtt taacatcagc
    22321 gccacagaaa aagccgcaac gccggtcatt cctcctgctg atgacaacgc ctttaacggc
    22381 agcctgagcg atgaaacagc ggtgatggca tggctgaaaa agcggattgc ggttcagcta
    22441 aggctgagcg atccggcgtc actgcatcca aaccaggatc tgttgcaact cggcatggac
    22501 tcgctgctct tccttgaact cagtagcgat attcagcact acctgggtgt acgcatcaat
    22561 gcggaacggg cgtggcagga tctgtctcct catggactca cgcagcttat ctgttctaag
    22621 ccagaggcga cgcctgccgc ttcgcagccg gaagtgttgc ggcacgacgc cgacgagcgt
    22681 tatgcgccct tccctttgac gcccattcag cacgcctact ggctggggcg aacccacctc
    22741 attggctatg gcggcgtcgc ctgtcacgtc ctgtttgagt gggataaacg ccacgatgag
    22801 ttcgatctcg ccatactgga gaaagcatgg aaccagctca tcgcacgcca cgatatgttg
    22861 cgtatggtgg ttgatgccga cgggcagcag cgaatcctgg cgacaacgcc ggagtatcac
    22921 atcccgcgtg acgatctgcg cgcgctttcc ccggaagaac agcgcatcgc gctggaaaaa
    22981 cggcggcatg aactgagcta tcgcgttttg cctgccgacc agtggcctct ttttgagctg
    23041 gtggtcagcg aaatcgacga ttgccattac cgtctgcata tgaacctcga ccttttgcag
    23101 tttgatgtgc agagttttaa agtcatgatg gacgacctgg cgcaggtctg gcgcggtgaa
    23161 acgctggcac cgctcgctat taccttccgt gattatgtga tggctgaaca ggcgcgccga
    23221 cagacatcgg catggcacga tgcctgggat tactggcagg aaaaactgcc gcaactgccc
    23281 ttagcgccag agctgccggt ggttgagacg cccccggaaa cgccacactt caccaccttc
    23341 aaatcgacga tcggcaagac agaatggcag gccgtgaaac agcgctggca gcagcaaggc
    23401 gtcacaccgt ctgccgcgct gctcacgctg tttgccgcca cccttgagcg ctggagccgt
    23461 accacaacat ttacgctgaa cctgacgttc ttcaatcgcc agccgatcca tccgcaaatc
    23521 aaccagttga ttggtgattt tacctccgtc acgctggttg attttaactt ctcagcgccg
    23581 gtgacgttgc aagagcagat gcaacagacc caacagcgcc tctggcaaaa catggcgcac
    23641 agtgaaatga acggtgttga ggtgatccgt gagctgggcc gcctgcgcgg atcacaacgt
    23701 caaccgctga tgccggtagt gtttaccagt atgctgggga tgacgctgga aggcatgact
    23761 atcgatcagg cgatgagcca tctgttcggc gaaccctgct atgtattcac gcaaacgccg
    23821 caggtctggc tggatcatca ggtcatggag agcgacggcg agttgatgtt tagctggtac
    23881 tgcatggaca acgtgctgga acccggcgct gccgaggcga tgtttaatga ctattgcgcc
    23941 atcctgcaag ccgtcatcgc cgcccctgaa agcctgaaga ctctcgccag cggcatcgcc
    24001 aggcacattc cccgccgacg ctggccgctg aacgcgcagg cggactacga cctgcgggat
    24061 attgagcagg cgacgctcga ataccccggc atccggcagg ccagagcgga aataaccgaa
    24121 cagggcgcgt tgacgctgga tatcgtgatg gccgacgatc cttcgccatc agcggcgatg
    24181 cctgatgagc acgaacttac ccaactggcg ctgccgttgc ctgagcaggc gcagcttgat
    24241 gagctggagg cgacctggcg ctggctggag gcgcgtgcgc tacaggggat cgcggctacg
    24301 ctaaatcgtc acggcctgtt taccacgccg gagatcgccc atcgctttag cgcaatagta
    24361 caggcgctgt ccgcgcaagc gtctcaccag cgtctgctgc gccagtggct acagtgtctg
    24421 acggaaagag agtggttaat ccgcgaaggt gaaagctggc gctgccgcat tccgctcagc
    24481 gagattcctg agcctcagga agcgtgcccg caaagccaat ggagccaggc gctggcgcag
    24541 tatctggaaa cctgcatcgc ccggcacgac gccctcttct ccgggcagtg ttctccgctg
    24601 gaattgctgt tcaacgagca gcatcgcgtt accgacgcgc tgtatcgcga caaccccgcc
    24661 agcgcctgtc tgaatcgcta taccgcgcag attgccgcct tgtgcagcgc agaacggatt
    24721 ctggaggttg gcgccggaac cgcagccact accgcgccgg tgctgaaggc cacgcggaac
    24781 acgcgacagt cgtaccactt cacggacgtc tccgcgcagt tcctcaatga cgccagagcc
    24841 cgtttccatg atgaatcgca ggtgtcttat gccttgttcg acatcaacca gccgctggat
    24901 ttcaccgccc acccggaggc gggttacgac ctgatcgttg ccgtcaatgt gctccacgac
    24961 gccagccatg tcgtccagac gttgcgcaga ttaaaactgt tgctgaaagc cggcggacgt
    25021 ttgctgatcg ttgaagcgac ggagcgaaac agcgtattcc agctggcgag cgtgggcttt
    25081 attgagggat taagcggata ccgcgatttc cgccgccggg atgagaaacc gatgctcacc
    25141 cgctccgcat ggcaggaggt tctcgttcag gccgggtttg caaacgagct ggcgtggccc
    25201 gcgcaggaat cgtcgccgct gcgccagcat ctgctggtag cgcgttcgcc tggcgtaaat
    25261 cgcccggata aaaaagccgt gagccgctat ttacagcagc gctttggcac cggtctgccc
    25321 attttacaga tccggcaaag agaagcgtta tttacgccgc tgcatgcccc gtctgatgcg
    25381 ccgactgagc cagccaaacc cacgccagtt gccgggggga atccggcact ggaaaaacag
    25441 gtggctgaac tctggcaatc gctgctgtct cgccccgtgg caaggcatca cgactttttc
    25501 gaactgggcg gcgacagcct gatggcgaca aggatggtcg cgcagctaaa ccggagaggg
    25561 attgccaggg ctaaccttca ggatctgttc agccattcga cgctgagcga cttctgcgcc
    25621 catctacagg cggctacgtc aggagaggac aacccgatac ccctttgcca gggcgacggt
    25681 gaggaaaccc tgtttgtctt ccacgcttca gacggcgata tcagcgcctg gctgccgctc
    25741 gctagcgcgt tgaacaggcg cgttttcggc ctgcaagcaa aatcgccgca gcgctttgcc
    25801 acgctcgacc agatgatcga tgagtatgtc gggtgcatcc gtcgtcagca gcctcacggc
    25861 ccttatgtgc tggcgggttg gtcgtatggc gcgtttcttg cggcgggcgc cgcacaacgc
    25921 ctgtacgcca aaggcgagca ggttcggatg gtgttaatcg atcccgtgtg ccgacaggat
    25981 ttctgttgcg aaaaccgggc ggccctgctg cgcctgttag ccgaaggaca aacgcctctg
    26041 gcactgcccg aacatttcga ccagcagacg cccgacagcc agcttgccga ctttatcagc
    26101 ctcgctaaaa cggccggtat ggtgtcgcaa aacctgacgc tgcaagcggc agaaacgtgg
    26161 ctcgacaaca tcgcgcatct gctgcgttta ctgactgagc atacgccggg cgaaagcgtt
    26221 ccggtcccct gtctcatggt gtatgccgcc gggagacccg cgcactggac gccagcagaa
    26281 accgagtggc agggctggat aaacaacgcc gacgacgctg tgattgaagc cagccactgg
    26341 caaatcatga tggaagcccc ccacgttcag gcttgtgcgc aacacattac gcgctggctt
    26401 tgcgcaacct caacgcaacc ggagaacacg ttatgatgcc gtccgcctcc ccaaaacaac
    26461 gcgtactgat tgtgggcgcc aaatttggcg aaatgtacct gaatgccttt atgcagcccc
    26521 cggaggggct ggaactggtc ggcctgctgg cgcagggaag cgctcgttca agagagctgg
    26581 ctcatgcgtt tggcattccg ctgtatacct cgccggaaca gataaccagg atgccggata
    26641 tcgcctgtat tgtcgtgcgt tcgaccgtcg ccggaggaac cggtacgcag cttgccagac
    26701 actttctgac gcgcggcgta cacgtcattc aggagcatcc cctccacccg gatgacatca
    26761 gttccttaca aacgctggcg caggaacagg gctgctgcta ttgggtaaac acgttttatc
    26821 cgcatacgcg cgcgggacgc acatggcttc gcgatgcgca gcagctgcgt cgctgtctgg
    26881 ctaagacgcc gcaggtggtc cacgccacca ccagccgaca gttgctctac tccactctgg
    26941 atttgttgtt gctggctctg ggcgttgatg ccgccgccgt ggagtgcgac gtggttggca
    27001 gctttagcga ttttcactgt ctgagactct tctggccgga gggagaagcc tgcctgctgc
    27061 ttcagcgcta tctcgatcct gacgatccag atatgcatag ccttatcatg caccgcctgc
    27121 tgctgggctg gccggaaggt catctttcgc tggaggcgag ctacggcccc gtcatctggt
    27181 catccagcct gtttgtcgca gaccatcagg agaacgccca cagcctctac cgcagaccgg
    27241 agatcctgcg cgatctgccg ggtctgacgc gcagcgcggc gcccctgagc tggcgcgact
    27301 gctgcgaaac cgtcgggccg gagggcgtca gctggttgct acaccagttg cgcagccatc
    27361 tggcgggcga acatccacca gcggcctgcc aaagcgtaca tcagatcgcg ttatcacgtt
    27421 tatggcagca gattttacgt aagaccggca atgcggagat cagacgcctg acgccgccgc
    27481 accacgaccg gttagccggt ttctacaacg atgatgataa ggaggcgctg tgacgcaatc
    27541 tgcaatgtgc atcccgctgt ggcccgcccg gaacggcaat actgcgcatc tggccatgtg
    27601 ccctttcgct ggcggcagca gtagcgcgtt tcgccactgg caagctgagc aactggctga
    27661 ttgcgcgctt tctctggtga cctggccggg gcgcgatcgc cttcgccatc tggaaccgct
    27721 cagaagcatt acacaactgg cggcactgct ggcgaacgag ctggaagcat ccgtatcgcc
    27781 tgacacgccg cttttactcg ccgggcacag catgggggcg caggtggcgt ttgaaacctg
    27841 ccgacttctg gagcaacggg ggcttgcgcc acaaggactg attatttccg ggtgccatgc
    27901 cccgcatctg cattctgaac gccagctcag ccatcgcgat gatgccgact ttatcgctga
    27961 gctgatagac attggcggat gttctcctga actgcgggaa aaccaggaat taatgtcgct
    28021 gtttcttcct cttctgcgcg ctgattttta cgccaccgag agctatcact acgactcgcc
    28081 cgacgtctgt ccgccgctgc gcacgcctgc gctgttattg tgcggcagcc acgatcgcga
    28141 agcctcctgg cagcaggtcg atgcctggcg tcagtggctg agccacgtta caggcccggt
    28201 ggtgattgac ggcgatcact tctatcccat tcaacaagcc cggtcctttt ttacgcagat
    28261 tgtccgccat tttccccacg cattttctgc aatgaccgcg tggcaaaaac agcccagtac
    28321 ttcagaaagg tgacgcatga attcttcctt tgaatctctg attgaacagt atcccttacc
    28381 cattgccgaa cagttgcgcc actgggcggc ccgttatgcc tcgcgaattg ccgtcgttga
    28441 tgcaaagggg tcgttaacct acagcgcgct tgatgcacaa gttgacgaac ttgccgcagg
    28501 tctgtcatca ctgggtttgc gttcggggga gcatgtaatt gtgcagcttc ccaacgacaa
    28561 cgcgtttgtt accctgctgt tcgccttgtt aagactgggc gttatccccg tgctggcgat
    28621 gccctcgcaa cgggcgctgg atatcgacgc gctgattgag ctggcgcaac ccgtcgctta
    28681 cgttattcac ggggaaaacc acgcagagct ggcccgacag atggcgcaca aacacgcctg
    28741 cttgcgtcat gttctggtcg ctggagagac catgagcgac gattttacgc cgctcttctc
    28801 ccttcacggt gagcgacagg catggccgca gcctgatgtt tccaccaccg cgttgttgtt
    28861 gctctcaggc ggcacaaccg gcacgcccaa actcatcccg cgccgacatg ccgactatag
    28921 ctataacttc agcgcttctg ctgaactgtg cggcatcagc caacagagcg tgtatctcgc
    28981 cgtcctcccg gtggcgcata actttccgct ggcctgcccc ggtattctgg gaacgcttgc
    29041 ctgcggcgga aaagtggtgc tgaccgacag cgccagctgt gatgaggtga tgcctttaat
    29101 cgcgcaggaa agagtgactc acgtcgccct ggttccggcg ctggcgcaat tatgggtgca
    29161 ggccagggag tgggaagaca gcgacctttc gtcgctgcgc gtcattcagg caggcggcgc
    29221 ccggctcgac ccgacgcttg ctgagcaggt tatcgccacc tttgactgta ccctgcaaca
    29281 ggttttcggt atggcggaag gcctgctctg ttttacccgg ctggacgatc cgcatgccac
    29341 cattctccac agccaggggc gcccgttgtc ccctctggat gaaatccgca tcgttgatca
    29401 agacgagaac gacgtcgcgc cgggcgaaac cgggcaattg ttaacgcgcg gcccttatac
    29461 catttcgggc tattaccgcg cccctgccca caacacgcag gcctttaccg cgcaagggtt
    29521 ttaccgcaca ggcgacaatg tcaggctgga tgaggtgggg aacctgcacg ttgagggacg
    29581 cataaaagag cagatcaacc gcgccggaga aaaaatagcc gcggctgaag tggaatcggc
    29641 actgctgcgt ttagcggaag tgcaagattg cgcggtggtc gccgcgccgg acacgctgct
    29701 tggcgagcgg atttgcgcgt ttatcatcgc gcagcaggtg ccaactgact atcagcagtt
    29761 gcgtcaacaa ctgacccgta tggggctcag cgcgtggaaa attcctgacc aaatcgagtt
    29821 tctggaccac tggccgctca ccgccgtcgg caagatagac aaaaaacgcc tgacggctct
    29881 cgccgtcgac cgttatcgcc attctgccca ataagcgcaa accgacccga aacaggttga
    29941 aataaacccg tttcgggtag caccaccatt agaaatagtt atcattttca attcaccatt
    30001 gtcggtattt tttggcgttt cgccgtctta cagggactca caacaatgaa aatgacacgg
    30061 ctttatcctc tggccttggg gggattattg ctccccgcca ttgctaatgc ccagacttca
    30121 cagcaagacg aaagcacgct ggtggttacc gccagtaaac aatcttcccg ctcggcatca
    30181 gccaacaacg tctcgtctac tgttgtcagc gcgccggaat taagcgacgc cggcgtcacc
    30241 gccagcgaca aactccccag agtcttgccc gggctcaata ttgaaaatag cggcaacatg
    30301 cttttttcga cgatctcgct acgcggcgtc tcttcagcgc aggacttcta taaccccgcc
    30361 gtcaccctgt atgtcgatgg cgtccctcag ctttccacca acaccatcca ggcgcttacc
    30421 gatgtgcaaa gcgtggagtt gctgcgaggc ccacagggaa cgttatatgg caaaagcgct
    30481 cagggcggga tcatcaacat cgtcacccag cagccggaca gcacgccgcg cggctatatt
    30541 gaaggcggcg tcagtagccg cgacagttat cgaagtaagt tcaacctgag cggccccatt
    30601 caggatggcc tgctgtacgg cagcgtcacc ctgttacgcc aggttgatga cggcgacatg
    30661 attaaccccg cgacgggaag cgatgactta ggcggcaccc gcgccagcat agggaatgtg
    30721 aaactgcgtc tggcgccgga cgatcagccc tgggaaatgg gctttgccgc ctcacgcgaa
    30781 tgtacccgcg ccacccagga cgcctatgtg ggatggaatg atattaaggg ccgtaagctg
    30841 tcgatcagcg atggttcacc agacccgtac atgcggcgct gcactgacag ccagaccctg
    30901 agtgggaaat acaccaccga tgactgggtt ttcaacctga tcagcgcctg gcagcagcag
    30961 cattattcgc gcaccttccc ttccggttcg ttaatcgtca atatgcctca gcgctggaat
    31021 caggatgtgc aggagctgcg cgctgcaacc ctgggcgatg cgcgtaccgt tgatatggtg
    31081 tttgggctgt accggcagaa cacccgcgag aagttaaatt cagcctacga catgccgaca
    31141 atgccttatt taagcagtac cggctatacc accgctgaaa cgctggccgc atacagtgac
    31201 ctgacctggc atttaaccga tcgttttgat atcggcggcg gcgtgcgctt ctcgcatgat
    31261 aaatccagta cacaatatca cggcagcatg ctcggcaacc cgtttggcga ccagggtaag
    31321 agcaatgacg atcaggtgct cgggcagcta tccgcaggct atatgctgac cgatgactgg
    31381 agagtgtata cccgtgtagc ccagggatat aaaccttccg ggtacaacat cgtgcctact
    31441 gcgggtcttg atgccaaacc gttcgtcgcc gagaaatcca tcaactatga acttggcacc
    31501 cgctacgaaa ccgctgacgt cacgctgcaa gccgcgacgt tttataccca caccaaagac
    31561 atgcagcttt actctggccc ggtcgggatg cagacattaa gcaatgcggg taaagccgac
    31621 gccaccggcg ttgagcttga agcgaagtgg cggtttgcgc caggctggtc atgggatatc
    31681 aatggcaacg tgatccgttc cgaattcacc aatgacagtg agttgtatca cggtaaccgg
    31741 gtgccgttcg taccacgtta tggcgcggga agcagcgtga acggcgtgat tgatacgcgc
    31801 tatggcgcac tgatgccccg actggcggtt aatctggtcg ggccgcatta tttcgatggc
    31861 gacaaccagt tgcggcaagg cacctatgcc accctggaca gcagcctggg ctggcaggcg
    31921 actgaacgga tgaacatttc cgtctatgtc gataacctgt tcgaccgtcg ttaccgtacc
    31981 tatggctaca tgaacggcag cagcgccgtc gcgcaggtca atatgggtcg caccgtcggt
    32041 atcaatacgc gaattgattt cttctgatta ttgtaaaagg gataccgaaa aggtatccct
    32101 tttacaccac tagttaaaac cagtaactca gcagagtcgc aaaaaatatt aatccatagt
    32161 gattatttaa acaatgaaat tgcgattagg ataaatagat ttaaa
//
LOCUS       DAHHTG010000047        32105 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.047, whole genome shotgun sequence.
ACCESSION   DAHHTG010000047 DAHHTG010000000
VERSION     DAHHTG010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 32105)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32105)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32105
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.047"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            363..548
                     /locus_tag="NBI74_002109"
     CDS             363..548
                     /locus_tag="NBI74_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001308408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ash family protein"
                     /protein_id="HCD1932449.1"
                     /translation="MCRAFGYTSMAGRAGASQDAPVSCNAGKANPVRFRPHEISLSSG
                     GHNKPLQETPYGYNPHL"
     gene            526..996
                     /locus_tag="NBI74_002110"
     CDS             526..996
                     /locus_tag="NBI74_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000201185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1932450.1"
                     /translation="MATILISDCDLLDLLSAPFNATTDFTVLADYCARFAETLIETDD
                     PALRLALCGRLHAALALLQPTLHDPIPPHLIESLTVDTLPATPEHFISEPDSLCEYCL
                     ALLQLLLERPAEPKTEQTLTGLLCELVWNFTEGLKSPRWLRTADGVKFISEVAA"
     gene            1142..1345
                     /locus_tag="NBI74_002111"
                     /pseudo
     CDS             1142..1345
                     /locus_tag="NBI74_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011579066.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
     gene            complement(1360..1435)
                     /locus_tag="NBI74_002112"
     tRNA            complement(1360..1435)
                     /locus_tag="NBI74_002112"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1400..1402),aa:Thr,seq:cgt)
     gene            complement(1550..2803)
                     /gene="proA"
                     /locus_tag="NBI74_002113"
     CDS             complement(1550..2803)
                     /gene="proA"
                     /locus_tag="NBI74_002113"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HCD1932451.1"
                     /translation="MLEQMGIAAKQASYKLAQLSSREKNRVLEKIADELEAQSESILN
                     ANAQDVADARANGLSEAMLDRLTLTPARLKGIADDVRQVCNLADPVGQVIDGGVLDSG
                     LRLERRRVPLGVIGVIYEARPNVTVDVASLCLKTGNAVILRGGKETCRTNAATVAVIQ
                     DALNSCGLPAGAVQAIDNPDRALVSEMLRMDKYIDMLIPRGGAGLHKLCREQSTIPVI
                     TGGIGVCHIYVDESAEIAEALKVIVNAKTQRPSTCNTVETLLVNKNIADSFLPALSKQ
                     MAESGVTLHADTAALAQLQAGPAKVVAVKAEEYDDEFLSLDLNVKIVSDLDDAIAHIR
                     EHGTQHSDAILTRDMRNAQRFVNEVDSSAVYVNASTRFTDGGQFGLGAEVAVSTQKLH
                     ARGPMGLEALTTYKWIGIGDYTIRA"
     gene            complement(2815..3918)
                     /gene="proB"
                     /locus_tag="NBI74_002114"
     CDS             complement(2815..3918)
                     /gene="proB"
                     /locus_tag="NBI74_002114"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HCD1932452.1"
                     /translation="MSDSQTLVVKLGTSVLTGGSRRLNRAHIVELVRQCAQLHAAGHR
                     IVIVTSGAIAAGREHLGYPELPATIASKQLLAAVGQSRLIQLWEQLFSIYGIHVGQML
                     LTRADMEDRERFLNARDTLRALLDNNIVPVINENDAVATAEIKVGDNDNLSALAAILA
                     GADKLLLLTDQKGLYTADPRSNPQAELIKDVYGIDDALRAIAGDSVSGLGTGGMSTKL
                     QAADVACRAGIDTIIAAGSKPGVIGDVMEGISVGTLFHAQATPLENRKRWIFGAPPAG
                     EITVDEGATAAILERGSSLLPKGIKSVTGNFSRGEVIRICNLEGRDIAHGVSRYNSDA
                     LRRIAGHHSQEIDAILGYEYGPVAVHRDDMITR"
     gene            4207..5262
                     /gene="phoE"
                     /locus_tag="NBI74_002115"
     CDS             4207..5262
                     /gene="phoE"
                     /locus_tag="NBI74_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoporin PhoE"
                     /protein_id="HCD1932453.1"
                     /translation="MKKSTLALVVMGIVASVSVQAAEIYNKDGNKLDVYGKVKAMHYM
                     SDNDSKDGDQSYIRFGFKGETQINDQLTGYGRWEAEFAGNKAESDTAQQKTRLAFAGL
                     KYKDLGSFDYGRNLGALYDVEAWTDMFPEFGGDSSAQTDNFMTKRASGLATYRNTDFF
                     GVIDGLNLTLQYQGKNENRDVKKQNGDGFGTSLTYDFGGSDFAISGAYTNSDRTNEQN
                     LQSRGTGKRAEAWATGLKYDANNIYLATFYSETRKMTPISGGFANKTQNFEAVAQYQF
                     DFGLRPSLGYVLSKGKDIEGIGDEDLVNYVDVGATYYFNKNMSAFVDYKINQLDSDNK
                     LNINNDDIVAVGMTYQF"
     gene            complement(5301..5702)
                     /gene="crl"
                     /locus_tag="NBI74_002116"
     CDS             complement(5301..5702)
                     /gene="crl"
                     /locus_tag="NBI74_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HCD1932454.1"
                     /translation="MTLPSGHPKSRLVKKFTALGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWTSVQIKDAEVVERLEHTLRE
                     FHEKLRELLATLNLKLEPADDFRDEPVKLTA"
     gene            complement(5760..7004)
                     /gene="frsA"
                     /locus_tag="NBI74_002117"
     CDS             complement(5760..7004)
                     /gene="frsA"
                     /locus_tag="NBI74_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HCD1932455.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFSHGAQLPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILEVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACAEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     ALRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDEA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITDWIEKRLC"
     gene            complement(7096..7554)
                     /gene="gpt"
                     /locus_tag="NBI74_002118"
     CDS             complement(7096..7554)
                     /gene="gpt"
                     /locus_tag="NBI74_002118"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HCD1932456.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQNTWIEQPWDMGVVFVPPISGR"
     gene            7815..9272
                     /gene="pepD"
                     /locus_tag="NBI74_002119"
     CDS             7815..9272
                     /gene="pepD"
                     /locus_tag="NBI74_002119"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HCD1932457.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRKPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFNLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDAL
                     KSLVDTYQEILKNELAEKEKNLALLLDSVANDKAALTATSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPAK"
     gene            complement(9328..9942)
                     /locus_tag="NBI74_002120"
     CDS             complement(9328..9942)
                     /locus_tag="NBI74_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor H"
                     /protein_id="HCD1932458.1"
                     /translation="MILLQLSSAQGPEECCLAVKKALDRLIKEATRQDVAVTVLETET
                     GRYSDTLRSALISLDGDNAWALSESWCGTIQWICPSPYRPHHGRKNWFLGIGRFTADE
                     QEQSDAIRYETLRSSGPGGQHVNKTDSAVRATHLASGISVKVQSERSQHANKRLARLL
                     IAWKLEQQQQENSAALKSQRRMFHHQIERGNPRRTFTGMAFIEG"
     gene            complement(9939..11078)
                     /locus_tag="NBI74_002121"
     CDS             complement(9939..11078)
                     /locus_tag="NBI74_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000521550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
                     /protein_id="HCD1932459.1"
                     /translation="MGKYIRPLSDAVFTIASDDLWIESLAIQQLHTTANLPNMQRVVG
                     MPDLHPGRGYPIGAAFFSVGRFYPALVGNDIGCGMALWQTDILARKYNADKFEKRLSD
                     LDDVAEESWLEENLPSAFAQHPWHNSLGSIGGGNHFAELQQIDQIIDAELFARAGLDA
                     QHLQLLVHSGSRGLGQSILQRHIASFSHHGLPEGSDDALRYIAEHDDALAFARINRQL
                     IALRIMQQVKATGSPVLDVAHNFVSACQIGDQQGWLHRKGATPDDNGLVIIPGSRGDY
                     SWLVKPVANEKTLHSLAHGAGRKWGRTECKGRLAAKYTATQLSRTELGSRVICRDKQL
                     IFEEAPQAYKSAESVVQCLVLAGLIIPVARLRPVLTLKNSGGKKG"
     gene            complement(11268..11720)
                     /locus_tag="NBI74_002122"
     CDS             complement(11268..11720)
                     /locus_tag="NBI74_002122"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCD1932460.1"
                     /translation="MNNIQIRNYQPGDFQQLCAIFLRAVTMTASQHYSPQQIAAWAQI
                     DESRWKEKLAKSQVRVAVINAQPVGFITRIGHYIDMLFVEPEYTRRGVASALLKPLIK
                     SESELTVDASITAKPFFERYGFQTVKQQRVECRGTWFTNFYMRYKPQH"
     gene            complement(11717..12772)
                     /gene="dinB"
                     /locus_tag="NBI74_002123"
     CDS             complement(11717..12772)
                     /gene="dinB"
                     /locus_tag="NBI74_002123"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HCD1932461.1"
                     /translation="MRKIIHVDMDCFFAAVEMRDNPALRDIPIAIGGSRERRGVISTA
                     NYPARKFGVRSAMPTGMALKLCPHLTLLPGRFDAYKEASNHIREIFSRYTSRIEPLSL
                     DEAYLDVTDSVHCHGSATLIAQEIRQTIFNELQLTASAGVAPVKFLAKIASDMNKPNG
                     QFVITPAEVPTFLQTLPLAKIPGVGKVSAAKLEAMGLRTCGDVQKCDLVMLLKRFGKF
                     GRILWERSQGIDERDVNSERLRKSVGVERTMAEDIHHWSECEAIIERLYPELERRLAK
                     VKPDLLIARQGVKLKFDDFQQTTQEHVWPRLNKSDLIATARKTWDERRGGRGVRLVGL
                     HVTLLDPQMERQLVLGL"
     gene            complement(12843..13628)
                     /gene="lafU"
                     /locus_tag="NBI74_002124"
     CDS             complement(12843..13628)
                     /gene="lafU"
                     /locus_tag="NBI74_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative lateral flagellar export/assembly
                     protein LafU"
                     /protein_id="HCD1932462.1"
                     /translation="MTTIKLIVNSVSKSERESIIAALHGQSIFNGGGLSPLNKISPSH
                     PPKPATVAVPEETEKKARDVNEKTALLKKKSATELGELATSINTIARDAHMEANLEME
                     IVPQGLRVLIKDDQNRNMFERGSAKIMPFFKTLLVELAPVFDSLDNKIIITGHTDAMA
                     YKNNIYNNWNLSGDRALSARRVLEEAGMPEDKVMQVSAMADQMLLDAKNPQSAGNRRI
                     EIMVLTKSASDTLYQYFGQHGDKVVQPLVQKLDKQQVLSQRTR"
     gene            13600..15312
                     /locus_tag="NBI74_002125"
                     /pseudo
     CDS             13600..15312
                     /locus_tag="NBI74_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140833.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
     gene            complement(15532..16029)
                     /gene="rayT"
                     /locus_tag="NBI74_002126"
     CDS             complement(15532..16029)
                     /gene="rayT"
                     /locus_tag="NBI74_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="REP-associated tyrosine transposase RayT"
                     /protein_id="HCD1932463.1"
                     /translation="MSEYRRYYIKGGTWFFTVNLQNRRNQLLTTQFQTLRNAIINVKR
                     DRPFEINAWVVLPEHMHCIWTLPEGDDDFSSRWREIKKQFTHACGLKNIWQPRFWEHA
                     IRNTQDYRHHVDYIYINPVKHGWVKQVSDWPFSTFHRDVARGLYPIDWAEDVTDFSAG
                     ERIIL"
     gene            complement(16205..16954)
                     /locus_tag="NBI74_002127"
     CDS             complement(16205..16954)
                     /locus_tag="NBI74_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000093946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HCD1932464.1"
                     /translation="MSSFLHGRFLHPGVFSLCVLLPLLASATTSHISFSYAARQRMQN
                     RARLLKQYQTHLKKQASYIVEGNAKSRRALRQHNREQIKQHPEWFPAPLKANDRRWQA
                     LAENNHFLSSNHLHNITEVAIHRLEQQLGKPYVWGGTRPDKGFDCSGLVFYAYNKILE
                     AKLPRTANEMYHYRRATIVANNDLRRGDLLFFHIHSREIADHMGVYLGDGQFIESPRT
                     GETIRVSRLAEPFWQDHFLGARRILTEETIL"
     gene            17266..18006
                     /locus_tag="NBI74_002128"
     CDS             17266..18006
                     /locus_tag="NBI74_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein L,D-transpeptidase"
                     /protein_id="HCD1932465.1"
                     /translation="MRKIALILAMLLIPCVSFAGLLGSSSSTTPVSKEYKQQLMGSPV
                     YIQIFKEERTLDLYVKMGEQYQLLDSYKICKYSGGLGPKQRQGDFKSPEGFYSVQRNQ
                     LKPDSRYYKAINIGFPNAYDRAHGYEGKYLMIHGDCVSIGCYAMTNQGIDEIFQFVTG
                     ALVFGQPSVQVSIYPFRMTDANMKRHKYSNFKDFWEQLKPGYDYFEQTRKPPTVSVVN
                     GRYVVSKPLSHEVVQPQLASNYTLPEAK"
     gene            complement(17977..18744)
                     /locus_tag="NBI74_002129"
     CDS             complement(17977..18744)
                     /locus_tag="NBI74_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HCD1932466.1"
                     /translation="MCELLGMSANVPTDICFSFTGLVQRGGGTGPHKDGWGITFYEGK
                     GCRTFKDPQPSFNSPIAKLVQDYPIKSCSVVAHIRQANRGEVALENTHPFTRELWGRN
                     WTYAHNGQLTGYKSLETGNFRPVGETDSEKAFCWLLHKLTQRYPRTPGNMAAVFKYIA
                     SLADELRQKGVFNMLLSDGRYVMAYCSTNLHWITRRAPFGVATLLDQDVEIDFSSQTT
                     PNDVVTVIATQPLTGNETWQKIMPGEWRLFCLGERVV"
     gene            complement(18949..19527)
                     /gene="lpcA"
                     /locus_tag="NBI74_002130"
     CDS             complement(18949..19527)
                     /gene="lpcA"
                     /locus_tag="NBI74_002130"
                     /EC_number="5.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-sedoheptulose 7-phosphate isomerase"
                     /protein_id="HCD1932467.1"
                     /translation="MYQDLIRNELNEAAETLANFLKDDANIHAIQRAAVLLADSFKAG
                     GKVLSCGNGGSHCDAMHFAEELTGRYRENRPGYPAIAISDVSHISCVGNDFGFNDIFS
                     RYVEAVGREGDVLLGISTSGNSANVIKAIAAAREKGMKVITLTGKDGGKMAGTADIEI
                     RVPHFGYADRIQEIHIKVIHILIQLIEKEMVK"
     gene            19767..22211
                     /gene="fadE"
                     /locus_tag="NBI74_002131"
     CDS             19767..22211
                     /gene="fadE"
                     /locus_tag="NBI74_002131"
                     /EC_number="1.3.8.7"
                     /EC_number="1.3.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase FadE"
                     /protein_id="HCD1932468.1"
                     /translation="MMILSILATVVLLGALFYHRVSLFISSLILLAWTAALGVAGLWS
                     AWVLVPLAIILVPFNFAPMRKSMISAPVFRGFRKVMPPMSRTEKEAIDAGTTWWEGDL
                     FQGKPDWKKLHNYPQPRLTAEEQAFLDGPVEEACRMANDFQITHELADLPPELWAYLK
                     EHRFFAMIIKKEYGGLEFSAYAQSRVLQKLSGVSGILAITVGVPNSLGPGELLQHYGT
                     DEQKNHYLPRLARGQEIPCFALTSPEAGSDAGAIPDTGIVCMGEWQGQQVLGMRLTWN
                     KRYITLAPIATVLGLAFKLSDPEKLLGGAEDLGITCALIPTTTPGVEIGRRHFPLNVP
                     FQNGPTRGKDVFVPIDYIIGGPKMAGQGWRMLVECLSVGRGITLPSNSTGGVKSVALA
                     TGAYAHIRRQFKISIGKMEGIEEPLARIAGNAYVMDAAASLITYGIMLGEKPAVLSAI
                     VKYHCTHRGQQSIIDAMDITGGKGIMLGQSNFLARAYQGAPIAITVEGANILTRSMMI
                     FGQGAIRCHPYVLEEMEAAKNNDVNAFDKLLFKHIGHVGSNKVRSFWLGLTRGLTSST
                     PTGDATKRYYQHLNRLSANLALLSDVSMAVLGGSLKRRERISARLGDILSQLYLASAV
                     LKRYDDEGRNEADLPLVHWGVQDALYQAEQAMDDLLQNFPNRVVAGLLNVVIFPTGRH
                     YLAPSDKLDHKVAKILQVPNATRSRIGRGQYLTPSEHNPVGLLEEALVDVIAADPIHQ
                     RICKELGKNLPFTRLDELAHNALTKGLIDKDEAAILVKAEESRLRSINVDDFDPEELA
                     TKPVKLPEKVRKVEAA"
     gene            complement(22254..22700)
                     /gene="ivy"
                     /locus_tag="NBI74_002132"
     CDS             complement(22254..22700)
                     /gene="ivy"
                     /locus_tag="NBI74_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ivy family C-type lysozyme inhibitor"
                     /protein_id="HCD1932469.1"
                     /translation="MFKAITTVAALVIATSAMAQDDLTISSLAKGETTKAAFNQMVQG
                     HKLPAWVMKGGTYTPAQTVTLGDETYQVMSACKPHDCGSQRIAVMWSEKSNQMTGLFS
                     TIDEKTSQEKLTWLNVNDALSIDGKTVLFAALTGSLENHPDGFNFK"
     gene            22881..23651
                     /gene="yafV"
                     /locus_tag="NBI74_002133"
     CDS             22881..23651
                     /gene="yafV"
                     /locus_tag="NBI74_002133"
                     /EC_number="3.5.1.111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutaramate amidase"
                     /protein_id="HCD1932470.1"
                     /translation="MPGLKITLLQQPLVWMDGPANLRHFDRQLEGITGRDVIVLPEMF
                     TSGFAMEAAASSLAQDDVVNWMTAKAQQCNALIAGSVALQTESDSVNRFLLVEPGGTV
                     HFYDKRHLFRMADEHLHYKAGNERVIVEWRGWRILPLVCYDLRFPVWSRNLNDYDLAI
                     YVANWPAPRSLHWQALLTARAIENQAYVAGCNRVGSDGNGCHYRGDSRVINPQGEIIA
                     TADAHQATRIDAELSMMALREYREKFPAWQDADEFTFR"
     gene            complement(23750..23826)
                     /locus_tag="NBI74_002134"
     tRNA            complement(23750..23826)
                     /locus_tag="NBI74_002134"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(23790..23792),aa:Asp,seq:gtc)
     gene            complement(23959..24690)
                     /gene="dnaQ"
                     /locus_tag="NBI74_002135"
     CDS             complement(23959..24690)
                     /gene="dnaQ"
                     /locus_tag="NBI74_002135"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HCD1932471.1"
                     /translation="MSTAITRQIVLDTETTGMNQIGAHYEGHKIIEIGAVEVVNRRLT
                     GNNFHVYLKPDRLVDPEAFGVHGIADEFLLDKPTFAEVADEFMDYIRGAELVIHNAAF
                     DIGFMDYEFSLLKRDIPKTNTFCKVTDSLAVARKMFPGKRNSLDALCARYEIDNSKRT
                     LHGALLDAQILAEVYLAMTGGQTSMAFAMEGETQQQQGETTIQRIVRQASKLRVVFAT
                     DEELAAHEARLDLVQKKGGTCLWRA"
     gene            24755..25222
                     /gene="rnhA"
                     /locus_tag="NBI74_002136"
     CDS             24755..25222
                     /gene="rnhA"
                     /locus_tag="NBI74_002136"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HCD1932472.1"
                     /translation="MLKQVEIFTDGSCLGNPGPGGYGAILRYRGREKTFSAGYTRTTN
                     NRMELMAAIVALEALKEHCKVILSTDSQYVRQGITQWIHNWKKRGWKTADKKPVKNVD
                     LWQRLDAALGQHQIKWEWVKGHAGHPENERCDELARAAAMNPTLEDTGYQVEV"
     gene            complement(25219..25941)
                     /locus_tag="NBI74_002137"
     CDS             complement(25219..25941)
                     /locus_tag="NBI74_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414749.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCD1932473.1"
                     /translation="MKPARVPQTVVAPDCWGDLPWGELYRKALERQLNPWFTKMYGFH
                     LLKIGNLSAEINCEACAVSHQVNVSAQGMPVQVQADPLHLPFADKSVDVCLLAHTLPW
                     CTDPHRLLREADRVLIDDGWLVISGFNPISLMGLRKLVPVLRKTSPYNSRMFTLMRQL
                     DWLSLLNFEVLHASRFHVLPWNKHGGKLLNAHIPALGCLQLIVARKRTIPLTLNPMKQ
                     SKNKPRIRQAVGATRQCRKPQA"
     gene            25975..26730
                     /gene="gloB"
                     /locus_tag="NBI74_002138"
     CDS             25975..26730
                     /gene="gloB"
                     /locus_tag="NBI74_002138"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HCD1932474.1"
                     /translation="MNLNSIPAFDDNYIWVLNDEAGRCLIVDPGDAEPVLNAISANNW
                     QPEAIFLTHHHHDHVGGVKELVEKFPQIVVYGPQETQDKGTTQVVKDGETAFVLGHEF
                     SVIATPGHTLGHICYFSKPYLFCGDTLFSGGCGRLFEGTPSQMYQSLRKLSALPDDTL
                     VCCAHEYTLSNMKFALSILPHDLSINDYYRKVKELRAKNQITLPVILKNERQINVFLR
                     TEDIDLINVINEETLLQQPEERFAWLRSKKDRF"
     gene            26802..28160
                     /gene="mltD"
                     /locus_tag="NBI74_002139"
     CDS             26802..28160
                     /gene="mltD"
                     /locus_tag="NBI74_002139"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase D"
                     /protein_id="HCD1932475.1"
                     /translation="MKAKAILLASVLLVGCQSTGNVQQHAQSLSAAGQGEAAKFTSQA
                     RWMDDGTSIAPDGDLWAFIGDELKMGIPENDRIREQKQKYLRNKSYLHDVTLRAEPYM
                     YWIAGQVKKRNMPMELVLLPIVESAFDPHATSGANAAGIWQIIPSTGRNYGLKQTRNY
                     DARRDVVASTTAALNMMQRLNKMFDGDWLLTVAAYNSGEGRVMKAIKTNKARGKSTDF
                     WSLPLPQETKQYVPKMLALSDILKNSKRYGVRLPTTDESRALARVHLSSPVEMAKVAD
                     MAGISVSKLKTFNAGVKGSTLGASGPQYVMVPKKHADQLRESLASGEIAAVQSTLVAD
                     NTPLNSRVYTVRSGDTLSSIASRLGVSTKDLQQWNKLRGSKLKPGQSLTIGAGSSAQR
                     LANNSDSITYRVRKGDSLSSIAKRHGVNIKDVMRWNSDTANLQPGDKLTLFVKNNNMP
                     DS"
     gene            complement(28209..28979)
                     /locus_tag="NBI74_002140"
     CDS             complement(28209..28979)
                     /locus_tag="NBI74_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCD1932476.1"
                     /translation="MTTQSHHDHVEKQFSSQASEYLTSTVHASGQDLQRLAVRLADYP
                     DASVLDMGCGAGHASFVAAQNVSTVVAYDLSAHMLDVVAQAAEARQLKNITTRQGYVE
                     SLPFADNAFDIVISRYSAHHWHDVGAALREVNRVLKPGGLLIVMDVMSPGHPVRDIWL
                     QTVEALRDTSHIRNHASGEWLTLISEANLIVDNLITDKLPLEFSSWVARMRTPEALVD
                     AIRIYQQSASTEVKTYFALQNDGSFTSDIIMLEAHKAA"
     gene            complement(29057..29857)
                     /locus_tag="NBI74_002141"
     CDS             complement(29057..29857)
                     /locus_tag="NBI74_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HCD1932477.1"
                     /translation="MRKNTYAMRYVAGQPAERILPPGSFASIGQALPPGEPLSTEERI
                     RILVWNIYKQQRAEWLSVLKNYGKDAHLVLLQEAQTTPELVQFATANYLAADQVPAFV
                     LPQHPSGVMTLSAAHPVYCCPLREREPILRLAKSALVTVYPLPDTRLLMVVNIHAVNF
                     SLGVDVYSKQLLPIGDQIAHHSGPVIMAGDFNAWSRRRMNALYRFAREMSLRQVRFTD
                     DQRRRAFGRPLDFVFYRGLNVSEASVLVTRASDHNPLLVEFSPGKPDK"
     gene            30098..31012
                     /locus_tag="NBI74_002142"
     CDS             30098..31012
                     /locus_tag="NBI74_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCD1932478.1"
                     /translation="MKATSEELAIFVSVVESGSFSRAAEQLGQANSAVSRAVKKLEMK
                     LGVSLLNRTTRQLSLTEEGERYFRRVQSILQEMAAAESEIMETRNTPRGLLRIDAATP
                     VVLHFLMPLIKPFRERYPEVTLSLVSSETIINLIERKVDVAIRAGTLTDSSLRARPLF
                     NSYRKIIASPDYISRYGKPETIDDLKQHICLGFTEPASLNTWPIACSDGQLHEVKYGL
                     SSNSGETLKQLCLSGNGIACLSDYMIDKEIARGELVELIADKVLPVEMPFSAVYYSDR
                     AVSTRIRAFIDFLSEHVKTAPGGAVREA"
     gene            complement(31009..31812)
                     /gene="dkgB"
                     /locus_tag="NBI74_002143"
     CDS             complement(31009..31812)
                     /gene="dkgB"
                     /locus_tag="NBI74_002143"
                     /EC_number="1.1.1.346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgB"
                     /protein_id="HCD1932479.1"
                     /translation="MAIPAFGLGTFRLKDDVVISSVKTALELDYRAIDTAQIYDNEAA
                     VGQAIAESGVPRHELHITTKIWIENLSKDKLIPSLKESLQKLRTDYVDLTLIHWPSPN
                     DEVSVEEFMQALLEAKKQGLTREIGISNFTIPLMEKAIAAVGAENIATNQIELSPYLQ
                     NRKVVAWAKQHSIHITSYMTLAYGKALKDEVIARIAAKHNATPAQVILAWAMGEGYSV
                     IPSSTKRENLESNLKAQNLQLDAEDKKAIAALDCNDRLVSPEGLAPEWD"
     gene            complement(31975..32051)
                     /locus_tag="NBI74_002144"
     tRNA            complement(31975..32051)
                     /locus_tag="NBI74_002144"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(32015..32017),aa:Asp,seq:gtc)
BASE COUNT         7909 a         8252 c         8142 g         7802 t
ORIGIN      
        1 aatacgcata tcactaattg tgaaatcata tgccccactc ctgatgttgt ggcgttattc
       61 gagaataaaa tgatgccaat ttataataaa ctggcatcaa acactcaaga gaatagtcat
      121 ttaatccaac tccgcgactg gctcctccct ctacttatga acggacaggt cacggtcaaa
      181 taatccccgg cgtggttttg cgaggcgcat tccagatccg cattgcgccg cactcaccgc
      241 agcaaaaccg cttgccgttc ataaccgatc gcagtatctt tctcccgcgc ctgcaaaatc
      301 aggcgtcggg attagcctcc tgaaaaccgt tacaggcgac acttgacgca ccagcgtctt
      361 ttttgtgtcg tgcgttcggc tacacctcaa tggcgggccg ggcgggggcg tcgcaagacg
      421 cgccggtttc ctgtaacgcc ggtaaggcta accccgtccg gttccgcccc catgagatta
      481 gcctctctag tggcggacat aataaaccgt tacaggagac gccctatggc tacaatcctc
      541 atctctgact gcgatttact cgatctattg tccgctccct tcaatgccac cacggatttc
      601 accgtcctgg ccgactactg cgcccgcttc gccgaaaccc tgatagaaac cgacgatccg
      661 gcgctgcggc tggcgctctg cggacggctt cacgcggctc ttgcgctatt gcaacccacg
      721 ctgcacgacc ccatcccgcc gcatctgatt gaaagcctga ccgttgatac ccttcccgcc
      781 acaccagagc actttatttc cgaaccagat tccttgtgcg aatactgcct cgcgctgctc
      841 cagctactgc tggaacgtcc agcagagcca aaaacggaac aaaccctgac cgggcttctg
      901 tgtgagctgg tctggaattt cacggaagga ctcaaatccc cgcgctggct gcgtaccgcc
      961 gacggcgtaa aatttatcag tgaggtggcc gcatgatgca acaggactga cattccgccg
     1021 acattattgc ggtgctgaaa aagcggggaa cgcctctggc ggcacagaga aaaactcatt
     1081 gcggaagcgc tgtaaatatc gccagaaatc atctggtcct caaggtactt tgatagcaaa
     1141 ggcaagctgg ctggttcagt ccggagtctc tcttcttgca cttaaagaga tgggaggatg
     1201 ggaaactctt gaaatggttc gtcgttaggc tcacctttca gccaggcatt tcaccgggca
     1261 cgcgagcaaa atcgatgcga ttataagtcg caatggcaca aatacggcac aagaggagaa
     1321 cgtggtgtat ttaaatatgg ggtaactact tgattttaat ggtgccgata ataggagtcg
     1381 aacctacgac cttcgcatta cgaatgcgct gctctaccaa ctgagctata tcggccctga
     1441 aaggacatgt tcacgaacgt gaatcacggt ggacaaggtt aaaactaacc gggcgatgcg
     1501 tcaatggcct tgtgaatcaa atggctactt ttgcatcacc cggttttatt tacgcacgaa
     1561 tggtgtaatc accaatgccg atccacttgt aagtggtcag tgcttccagc cccattgggc
     1621 cgcgcgcgtg gagtttttgt gtgcttaccg ccacttccgc ccccagtcca aactggccgc
     1681 cgtcggtaaa acgcgtagag gcgttaacgt aaacagcgga ggaatccact tcgttaacaa
     1741 aacgctgggc gttgcgcata tcgcgggtca ggatcgcatc ggagtgttgc gtgccgtgtt
     1801 cacgaatatg ggcaatggcg tcatccagat cgctaacgat tttgacgttc aaatctaatg
     1861 acagaaactc atcgtcatac tcttcggctt taacagccac caccttcgcg gggcctgcct
     1921 gcaactgcgc cagcgcggct gtatcagcgt gtaacgtcac gccgctttcc gccatttgtt
     1981 tgcttaatgc gggcaggaag ctatcagcga tgtttttatt caccagcaac gtttctaccg
     2041 tattacacgt gctcggacgc tgagttttcg cgttgacaat tactttcagg gcttcagcga
     2101 tctctgcgct ttcatcaacg taaatatggc atacgcctat accgcctgtg atcaccggga
     2161 ttgtcgactg ctcgcggcac agtttatgca aaccagcccc gccgcgcggg atcagcatgt
     2221 cgatgtattt atccatacgc agcatttcac tgaccagcgc acggtcagga ttatcaatcg
     2281 cctgcacggc acccgccggt aagccacaag agttcagggc gtcctgaatc accgccaccg
     2341 ttgcagcgtt agtgcgacac gtttctttgc cgccgcgcag gatcactgcg ttacctgttt
     2401 tcaggcacag cgaagcgaca tcaaccgtca cgttcgggcg cgcttcataa atcacgccaa
     2461 taacccccag cggtacgcga cgacgctcaa gacgcaggcc gctgtccagt acgccgccat
     2521 cgattacctg ccccaccgga tcggcgaggt tgcacacctg gcgcacatca tcggcaatgc
     2581 ctttcagccg tgcgggcgtc agtgtcagac ggtcaagcat cgcttcgcta aggccattgg
     2641 cacgcgcgtc tgcaacatcc tgcgcgttag cgttgaggat actttcgctt tgtgcttcca
     2701 gttcatcggc gattttttcc agcacacgat ttttttcgcg gctggagagt tgcgctaatt
     2761 tatacgaggc ttgcttcgcg gcaatgccca tttgttccag catcggccag ctccttaacg
     2821 ggtaatcatg tcatcacggt gaacggcaac cgggccgtat tcatatccca gtattgcatc
     2881 aatttcttgc gagtggtgtc cggcaatacg gcgtaatgca tcgctgttgt aacgactgac
     2941 gccgtgggcg atatcgcgac cttcgaggtt gcaaatacgg atgacttcac cacgcgagaa
     3001 attgccagtc acgcttttaa tgcctttcgg caacagggag ctgccgcgtt ccagaatggc
     3061 ggcagttgcc ccttcatcta ccgtgatttc acccgcaggc ggcgcaccga aaatccagcg
     3121 tttacggttt tcaagcggag tcgcctgggc atggaacagc gtaccgacgg aaatgccttc
     3181 catcacatca ccaataacgc ccggcttgct gcccgcggca ataatggtgt cgatacccgc
     3241 acggcaagcc acgtcagcgg cctgcaattt ggtgctcata ccgccagttc cgaggcctga
     3301 aacgctatca ccggcaatcg cgcgcagtgc gtcatcaatg ccgtacacat ctttaatcag
     3361 ttctgcctgc ggattgctgc gcgggtcagc ggtatacaaa cctttttgat cggtcagcag
     3421 caacagttta tcggcacccg ccagaatcgc cgccagcgca gaaaggttat cgttatcacc
     3481 gaccttaatc tctgccgtag cgacagcatc gttctcatta attaccggaa cgatattgtt
     3541 atcgagcaac gcacgcaggg tatcgcgggc gttcaggaag cgttcacgat cttccatatc
     3601 agcacgggtc agcaacatct gcccgacatg aatgccgtaa attgaaaaca gctgttccca
     3661 cagttgaatc agtcgactct gccctaccgc cgccagcagc tgttttgaag cgatagtcgc
     3721 tggcaattcc gggtacccca ggtgctcacg tccggcggca atcgcacccg acgtcacaat
     3781 aacaatccga tgcccggcgg catgtaactg cgcgcactgg cgaacaagtt caacgatatg
     3841 ggcacggttc aggcggcgcg atccgcctgt tagcacactg gtgccgagtt ttaccaccag
     3901 cgtctggctg tcactcatga ttctctgcca ttcaatttta ggaaaaaatg atatcaaacg
     3961 aacgttttag caggactgtc gtcggttgcc aaccatctgc gagcaaagca tggcgttttg
     4021 ttgcgcggga tcagcaagcg tagcggcagt tgtttacgct tttattacag atttaataaa
     4081 ttaccacatt ttaagaatat tattaatctg taatatatct ttaacaatct caggttaaaa
     4141 actttcctgt tttcaacggg actctcccgc tgaatattcg cgcgttaatt aaaatcagga
     4201 atgaaaatga aaaagagcac tctggcatta gtggtgatgg gcattgtggc atctgtatcc
     4261 gtacaggccg cagaaatata taacaaagac ggtaataaac tggatgtcta tggcaaagtt
     4321 aaagccatgc attatatgag tgataatgac agtaaagatg gcgaccagag ttatatccgt
     4381 tttggtttta aaggcgaaac acaaattaac gatcaactga ctggttatgg tcgttgggaa
     4441 gctgagtttg ccggtaataa agcggagagt gatactgcac agcaaaaaac gcgtctcgct
     4501 tttgccgggt tgaaatataa agatttgggt tctttcgatt atggtcgtaa cctgggcgcg
     4561 ttgtatgacg tggaagcctg gaccgatatg ttcccggaat ttggtggcga ctcctcggcg
     4621 cagaccgaca actttatgac caaacgcgcc agcggtctgg cgacgtatcg gaacaccgac
     4681 ttcttcggcg ttatcgatgg cctgaactta accctgcaat atcaagggaa aaacgaaaac
     4741 cgcgacgtta aaaagcaaaa cggcgatggc ttcggcacgt cattgacata tgactttggt
     4801 ggcagcgatt tcgccatcag tggagcctat accaactctg atcgcaccaa cgagcagaac
     4861 ctgcaaagcc gtggcacagg caagcgtgca gaagcatggg caacaggtct gaaatacgat
     4921 gccaataata tttatctggc aactttctat tctgaaacac gtaaaatgac gccaatctct
     4981 ggcggctttg ccaataagac gcagaacttt gaagcggtcg ctcaatacca gtttgacttt
     5041 ggtctgcgtc catcgctggg ttatgtctta tcgaaaggga aagatattga gggcatcggt
     5101 gatgaagatc tggtcaatta tgtcgatgtt ggtgctacgt attatttcaa caaaaatatg
     5161 tcagcgtttg ttgattataa aatcaaccaa ctggatagcg ataacaaatt gaatattaat
     5221 aatgatgata ttgtcgcggt tggcatgacc tatcagtttt aatggatatt gccggatgtg
     5281 atgcatccgg cacatttcat tcacgccgtt aacttcaccg gttcatcacg aaaatcatct
     5341 gccggttcca gcttcagatt cagcgtcgcc agcagctcac gcagcttctc gtgaaattca
     5401 cgcagggtgt gctccagtcg ttcaaccact tcggcatctt ttatctgaac actcgtccag
     5461 tcgcctgctt tatcgaacag accaaactgg taactgtagg taaaacggga ttcctgcgct
     5521 tccagttcca tccaccagcc ccagaactca cgcacttccg gtgccggttt cacgttgacg
     5581 catacagcca gacaatcgaa aaagaatcga ttatcttcgc acttaccttc acgaatatac
     5641 gggcctagtg cggtaaattt tttgactaat ctgctcttcg ggtgtccact cggtaacgtc
     5701 attgcaatct cctgttgtga tgcaactgtt ttaccaaatt ggtaaaattt agcaaatttt
     5761 taacacaaac gtttttcgat ccagtctgtg atttcctgaa ggcctttgtc aaaattccga
     5821 tacaccgggt taaatgggat ctctaataat ttaccgtcag cagatgacga cgtgattaag
     5881 cgtgagtcct cttccgggct gaacggatcg ttcttccagt agcctgataa cattggcgtt
     5941 gggcagcgac gtcccagcaa cccttgcact tttaatgaat agcgattcag ctccacgcgc
     6001 aacgcttcgt cagaagcatc atgcatcccc aaacgactcg ccagaacgtc aagatacatt
     6061 tccggcacct gttgctggca cttaaaatca ctcaacaggg tatgaactac cggaccaaga
     6121 caggcaaccg ctttcagacg cggcgattca aggtatgcca gacgtacggc aacgttagcg
     6181 ccgaaacgga aaccaaaggc cgcgacgcga gtgtgatcca cccacggtac gttaggcaac
     6241 gcctttaaaa cgtgctgatg caacaggctg gagtcctggg tgagcttcca ttttgaagaa
     6301 aagcccaccg acggcatatc aatagtcagc atcgcaatgc cgcgcagcgc aaaataacgt
     6361 tcatacaggc tgtaatagtc tgtctgcatc gcatccagac caccacacat taatactgtc
     6421 gggaacgggc catcgccttt cggcatatgc aaaaagccgg tgatgggcgc accgccgggt
     6481 acggtaaact ccatctgccg catcgtgccc ggcagacgct gagcggcctc ttcataggcg
     6541 cggtttgaca aagcctgcgc ttgctcggcc aggtcatctc ctttcagatg aggataggca
     6601 gcaatgttgt acaacgtagc cgcatgcagc cagtgtcgac cgctgagttg cggatcttct
     6661 tccgcacagg ctttttgttg ccacaccatc gcctgggtag cccactcata aatccagttg
     6721 ccgccgcggt agccaatcac cgtatcgtat aaatcatcgt cggtacgttc ggcatcgctc
     6781 atcacaatac gcgcctggac ttcgaggatt tcacgggggt caatgccgcg ccagatccac
     6841 atcagacggt taatcatgcg ataccagtga gggatggttt taccatcaag ggccgactgc
     6901 acaggcagtt gtgcgccgtg actaaagcgg cggactagcg tcgaggtttc aggatgttta
     6961 aagcggggtt tgaacagggt ttcgctcagg tttgcctgtg tcatagatgc agcctccaga
     7021 atacttactg gaaactattg taacccgcct gaagttaaaa agaacaacgc ccggcagtgc
     7081 caggcgttga aaagattagc gaccggagat tggcgggacg aatacgacgc ccatatccca
     7141 cggctgttcg atccaggtat tttgcgggat atcaacaaca taatcatcaa ccagcggacg
     7201 accagccggt tttgcgaaga tggtgacaaa gtgcgctttt ggatacattt cacgaatcgc
     7261 aaccgcagta ccaccggtat ctaccaggtc atcaataacg atgaagcctt cgccatcgcc
     7321 ttctgcgcgt ttcagcactt taagctcgcg ctggttgtcg tgatcgtagc tggaaataca
     7381 aacggtatcg acatgacgaa tacccagttc acgcgccagt aacgcacccg gtaccagacc
     7441 gccacggctt acggcaataa tgcctttcca ttgttcagaa ggcatcagtc ggcttgcgag
     7501 tttacgtgca tggatctgca acatgtccca ggtgacgatg tatttttcgc tcatgtgaag
     7561 tgtcccagcc tgtttatcta cggcttaaaa agtgttcgag gggaaaatag gttgcgcgag
     7621 attatagaga tctggcgcac taaaaaccag tatttcacat gagtccgcgt ctttttacgc
     7681 actgcctctc cctgacgcgg gataaagtgg tattctcaaa catatctcgc aagcctgtct
     7741 tgtgttgaca acattttctg ctaaccctgt gacctgcaat actgttttgc gggtgatcga
     7801 caaggagact taacgtgtct gaactgtctc aattatctcc acagccgctg tgggatattt
     7861 ttgccaaaat ctgttctatt cctcacccgt cctatcatga agagcaactc gctgaataca
     7921 ttgttggttg ggcaaaagag aaaggtttcc atgtcgaacg cgatcaggta ggtaatatcc
     7981 tgattcgtaa acctgctacc gcaggtatgg aaaatcgtaa accggtcgtc ttgcaggccc
     8041 acctcgatat ggtgccgcaa aaaaataacg acaccgtgca tgacttcact aaagatccta
     8101 tccagcctta tattgatggc gaatgggtta aagcgcgcgg caccacgctg ggtgcggata
     8161 acggcattgg tatggcctcc gcactggcgg ttctggctga cgaaaacgtg gttcacggcc
     8221 cgctggaagt gctgctgacc atgactgaag aagccggtat ggacggtgcg ttcggcttac
     8281 agagcaactg gttgcaggct gatattctga ttaacaccga ctccgaagaa gaaggtgaaa
     8341 tctacatggg atgtgcgggg ggtatcgact tcacctccaa cctgcattta gatcgtgaag
     8401 cggttccagc tggttttgaa accttcaatt taaccttaaa aggtctgaaa ggcggtcact
     8461 ccggcggtga aatccacgtt ggcctgggta atgccaacaa actgctggtg cgcttcctgg
     8521 cgggtcatgc agaagaactg gacctgcgtc ttattgattt caacggcggc acactgcgta
     8581 acgccatccc gcgtgaagcc tttgcgacca ttgctgtcgc agctgataaa gtcgacgccc
     8641 tgaaatctct ggtggatacc tatcaggaga tcctgaaaaa cgaactggca gaaaaagaga
     8701 agaatctggc attgctgctg gactccgtag cgaacgacaa agctgcatta acggcgacat
     8761 ctcgcgatac ctttattcgt ctgctgaacg ccaccccgaa cggagttatc cgtaattccg
     8821 atgtagccaa aggcgtggtt gaaacctccc tgaacgtcgg tgtggtgacc atgaccgaca
     8881 ataacgtaga aattcactgc ctgatccgtt ctctgatcga cagcggtaaa gactacgtag
     8941 tgagcatgct ggattcgctg ggtaaactgg caggtgcgaa aaccgaagca aaaggcgcat
     9001 atcctggctg gcagccggac gctaactctc cggtaatgca tctggtacgt gaaacctatc
     9061 agcgtctgtt caacaagacg ccgaacatcc agattatcca cgcgggcctg gaatgtggtc
     9121 tgttcaaaaa accgtatccg gaaatggaca tggtgtctat cgggccaacc atcaccggtc
     9181 cacactcccc ggatgagcaa gttcatatcg aaagcgttgg ccattactgg acactgctga
     9241 ctgaactgct gaaagaaatt ccggcgaagt aattatttga tttgctgccg gatggcgttt
     9301 aacgccttcc ggcagtttca tccttcatta tccttcgata aaagccatcc ctgtgaacgt
     9361 tcgtcgcggg ttgccacgtt caatctggtg atggaacatt cgccgctgcg atttcagcgc
     9421 cgcgctattt tcctgttgct gttgttccag cttccaggca atcagtaatc gcgccagccg
     9481 tttgttagca tgctgactgc gctctgactg aaccttcacg ctaataccgg atgccagatg
     9541 cgtggcgcgt accgccgagt cggttttatt gacatgttga ccgcccggcc ccgacgaacg
     9601 cagcgtctca taacggattg catccgattg ttcctgctca tcagcggtaa aacgcccaat
     9661 gcccagaaac cagtttttgc gcccatgatg aggccgatac ggactcggac aaatccactg
     9721 aatagtgccg caccagcttt cgcttagagc ccatgcgtta tcgccatcca gagaaatcag
     9781 cgccgaacgc agtgtgtcag agtagcgacc cgtttctgtt tccagcaccg ttaccgcgac
     9841 gtcctgtcgg gtagcttctt taatcagcct gtccagtgct tttttcactg cgagacaaca
     9901 ttcttccggc ccctgagcag aggagagttg tagcaagatc atcctttttt ccctccactg
     9961 tttttgagcg tcagcaccgg gcgcagtcgc gcgacaggaa taattaaccc agccagcacc
    10021 agacattgca ccacgctttc agccgattta taggcttgtg gcgcttcttc aaagatgagt
    10081 tgtttatcgc gacaaattac ccgactgcca agttcagtcc gcgagagttg cgtcgccgtg
    10141 tatttcgctg ccagacgccc tttacactcg gtgcgccccc atttacgccc cgccccatgc
    10201 gccagcgagt ggagcgtttt ttcgttcgca acaggtttaa ccagccagga gtaatcgccg
    10261 cgtgaaccgg gaataatcac cagaccgttg tcatccggcg tggcgccttt acgatgcaac
    10321 cagccctgtt gatcgccgat ttggcacgcg ctaacaaagt tatgcgccac atccagaacc
    10381 ggacttccgg tggccttaac ttgttgcatt atgcgcaaag cgatcagctg gcgattaata
    10441 cgcgcaaacg ccagcgcatc gtcatgttcc gcaatatacc gtagcgcgtc gtcactgcct
    10501 tcaggcaaac catgatgcga aaacgaggca atatgccgct gtaaaataga ctgccccaat
    10561 cccctcgagc cgctatgaac cagtagttgc agatgctgcg catccagacc agcccgtgca
    10621 aacagttccg cgtcgataat ttgatcaatc tgttgcagtt ctgcgaagtg gttaccgcca
    10681 ccgatggagc caagcgagtt gtgccacgga tgctgtgcaa atgctgacgg caggttttcc
    10741 tccagccagc tttcttcagc aacgtcatcc agatctgata atcgcttttc aaacttatcg
    10801 gcgttgtatt tgcgagcgag aatatctgtt tgccatagcg ccatgccgca gccgatatca
    10861 ttgccgacca gtgccgggta aaaacgacca acagagaaga acgctgcgcc aatcgggtag
    10921 ccgcgtccgg gatgtaaatc tggcatcccc acaacgcgct gcatgttggg taaatttgcc
    10981 gtggtgtgta attgttggat cgctaaactc tcgatccaca ggtcatcaga tgcgatggta
    11041 aataccgcat cggataaggg acgaatatat ttgcccatgt accattccat ttatgaataa
    11101 aaatcaggaa atggcagacg aatccagggc aagaaaaaag aattaatcct ggaaaggtct
    11161 gcaaagagtc attgataaag gatttgtcat gatttacctc cttgcagttt gtggaataag
    11221 aaaacgcgca catactagac gggcgttatt ttcattgcaa gctggattta atgttgcggt
    11281 ttatatcgca tataaaaatt agtaaaccac gttccccggc attcaacgcg ctgctgctta
    11341 actgtctgaa aaccataacg ttcaaaaaag ggttttgcgg ttatgctggc gtccaccgta
    11401 agttcggatt cagacttaat caatggtttt aacaaagcgc tggcaactcc gcgtcgggtg
    11461 tattccggtt caacaaataa catatcgata taatgtccaa tgcgggtaat aaaaccaacc
    11521 ggttgtgcat taataactgc aacccgcact tgtgatttcg cgagtttctc cttccagcga
    11581 gattcgtcaa tctgcgccca ggcggcaatt tgctgtggag aatagtgctg actcgccgtc
    11641 atcgtaacag ctcgaaggaa aatagcgcat agttgctgaa aatcaccagg ctgatagttt
    11701 cttatttgta tgttattcat aatcccagca ccagttgtct ttccatttgc gggtcaagca
    11761 acgtcacatg cagccccacc agtcgcacac cgcgcccgcc gcggcgttca tcccaggttt
    11821 tacgcgcggt ggcgattaga tcagatttat tcagccgcgg ccagacgtgt tcctgggtgg
    11881 tttgctgaaa atcgtcgaac tttaatttca ccccctggcg ggcaatcagc aaatcaggtt
    11941 ttacctttgc caaacgacgc tccagttcag ggtaaagccg ctcgatgatc gcttcacatt
    12001 cagaccagtg atgaatatct tccgccatcg tacgttctac accgacggat tttcgcaacc
    12061 tttcgctgtt aacgtcgcgt tcgtcaatgc cctgactacg ctcccacaaa atgcggccaa
    12121 atttgccaaa gcgtttaagc agcatcacca gatcacactt ttgcacatca ccgcaggtcc
    12181 gcagccccat cgcttccagt tttgccgccg agactttgcc gacgccggga atttttgcca
    12241 gcggtaaggt ttgtaaaaat gtcggaactt ctgccggcgt aatcacaaac tggccgttgg
    12301 gtttattcat gtcggaggcg attttggcga gaaactttac tggtgccacg cccgcagacg
    12361 ccgtcagttg cagctcgttg aagatcgtct ggcggatttc ctgggcgatg agggtcgcag
    12421 aaccgtggca atggacgcta tcggtgacat cgagataggc ttcatccagt gataacggtt
    12481 caatgcgcga ggtatagcgc gagaaaattt cgcggatatg atttgaggct tctttgtagg
    12541 cgtcaaagcg ccctggaagc aaggtgagat gcgggcataa tttgagcgcc atccctgtcg
    12601 gcatagcgct acgtacgcca aatttacgcg cgggataatt ggcggtgctg atcacccccc
    12661 gtcgttcgcg gctgccgcca atagcaatag ggatatcgcg cagggcggga ttgtcgcgca
    12721 tctccaccgc tgcgaaaaag cagtccatat ccacatgaat gattttacgc attgctcacc
    12781 tctcaatact ggtaaagtat acagtgattt cagggtttga gaaatgcgta aagattcagc
    12841 atttaacgcg ttcgctgcga aagcacctgc tgcttatcca gcttttgcac cagcggctgc
    12901 accactttat ccccatgctg accaaagtat tgatacagcg tatcggacgc acttttggtc
    12961 agcaccataa tctcaatgcg ccggttgccc gcgctttgcg gatttttggc atccagcagc
    13021 atctggtccg ccattgcact tacctgcatc actttatctt ccggcattcc ggcctcttcc
    13081 agcacccgac gagctgaaag cgcgcgatcg cccgaaaggt tccagttgtt gtagatattg
    13141 tttttgtagg ccatcgcatc ggtatgcccg gtaataataa ttttgttatc gagcgagtcg
    13201 aacactggcg caagctccac cagcaaagtt ttaaagaacg gcataatctt cgcgctgccg
    13261 cgttcgaaca tattgcggtt ctggtcgtct ttaatcagca cgcgtaatcc ctggggaaca
    13321 atctccatct ccagattcgc ttccatatgc gcatcacggg cgatggtatt gatactggtt
    13381 gccagctcgc caagctcggt ggcggatttc ttcttcagca gcgccgtttt ctcgttgacg
    13441 tcgcgcgctt ttttctccgt ctcttccggc accgccacgg tggcaggttt tggcggatga
    13501 gacgggctga ttttatttaa cggcgacaac ccaccaccgt taaaaatcga ctgcccgtgc
    13561 agcgcggcga taatgctttc acgctcggat ttactgacgc tgttgacaat caactttatc
    13621 gtcgtcacca aaggggccga gcgtatttcc gaggtttctg cccgctttac cctggacgcg
    13681 atgcccggca aacagatggc aattgacgcc gatcttaatg ccgggttgat caaccaggca
    13741 caggcgcaga cccggcgcaa agatgttgcc agcgaggccg atttttacgg cgcgatggac
    13801 ggggcatcga agtttgtgcg cggggatgcc atcgccggga tgatgattct ggcgatcaac
    13861 ctgatcggcg gcgtctgtat tgggatcttc aaatacaacc tgagcgccga tgctgccttc
    13921 cagcaatatg tgttgatgac catcggcgac ggcctggtgg cgcagatccc ttccctgctg
    13981 ctctccaccg cggcggcgat tatcgtcacc cgcgtcagcg acaacggcga tatcgcccat
    14041 gacgtgcgta atcagttgct ggcaagcccg tcggttctct acaccgccac tggaattatg
    14101 ttcgtgctgg cggtggtgcc gggaatgccg catttcccgt tcctgatgtt cagcgccctg
    14161 cttggtttta ccggctggcg gatgagcaag cgcccgcaag cggcggaggc ggaagagaaa
    14221 agcctcgaaa cgctaacccg tacgatgaca gaaaccagcg agcaacaggt cagttgggaa
    14281 actattccgc tgatcgaacc tatcagttta agcctcggct acaaactggt ggcgctggtg
    14341 gataaagcgc agggtaatcc gctcacccag cggattcgcg gtgtacggca ggtgatatcc
    14401 gacggtaacg gagttctgct gccggagatc cgtattcggg aaaacttccg cctcaagccc
    14461 agccagtacg ccattttcat caacggcatt aaggctgatg aagcggatat tccggcggat
    14521 aaactgatgg ccctgccctc cagcgaaacc tacggcgaga ttgacggcgt actggggaat
    14581 gacccggcgt acgggatgcc ggtcacctgg attcaacccg cgcagaaagc gaaggcgctg
    14641 aatatggggt atcaggtgat cgacagcgcc agcgtgattg ccacccatgt gaataagatt
    14701 gtgcgcagct atattcctga cctctttaac tatgacgata ttacgcagct gcataaccgt
    14761 ttgtcgtcga tggcaccgcg actggcggaa gatttgagcg cggcactcaa ttacagccag
    14821 ttgctgaaag tgtaccgggc gctactgact gaaggcgttt ccctgcgcga tatcgtcacc
    14881 atcgccaccg tgctggtcgc cagtagtgcg gtgaccaaag atcatattct gctggcggca
    14941 gatgtgcgcc tggcgctgcg gcgcagcatt acccatccgt tcgttcgcaa gcaggagctg
    15001 acggtgtata cgctgaataa tgagctggaa aatctgctga ccaacgtggt gaatcaggcg
    15061 caacagggcg ggaaagtgat gcttgatagc gtgccagtgg acccgaatat gctcaaccag
    15121 ttccagagca cgatgcctca ggtgaaggag cagatgaaag cggcggggaa agatccggtg
    15181 ctgctggtgc cgccgcaact gcgccccttg ctggcgcgct acgcaaggtt gtttgcgccg
    15241 gggctgcatg tgctgtcgta taacgaagtg ccggatgagc tggagttgaa gataatgggg
    15301 gcgttgatgt aggcatccgg catcggtgca tattgccgga tgcggcgtga acgccttatc
    15361 cggcctacgg gtgagcacgg ttttgtcggc atgataagac gcgtagcggc gcatctggca
    15421 ccggtgcaca actgccggat gcggcgtgaa cgccttatcc ggcctacggg tgagcgcggt
    15481 tttgtcggca tgataagacg catctgcgtc gcatcaggca tcggcgcaca gctataaaat
    15541 gatacgctcc ccagcactaa aatccgttac gtcctccgcc caatcgatgg gatataaccc
    15601 tcgcgcgaca tcgcgatgaa acgttgagaa tggccaatca ctcacttgct ttacccaacc
    15661 atgttttact ggatttatat aaatataatc aacatgatgg cgatagtctt gggtattgcg
    15721 aatggcatgc tcccaaaaac gcggttgcca gatatttttc aatccacaag catgggtaaa
    15781 ttgcttttta atttcccgcc agcgcgagga aaaatcatca tcgccttcag gtaatgtcca
    15841 tatgcaatgc atatgctccg gcaaaactac ccaagcgtta atttcaaaag gtctgtctcg
    15901 cttaacgtta ataatagcgt tacggagcgt ctgaaactgg gtggtcaaaa gttggtttcg
    15961 acgattctgt aaatttaccg tgaaaaacca ggttcccccc ttgatgtaat aacgacgata
    16021 ttcagacata acgactcctt tttctcaacg gtaagaagaa aaggaaaagt cggcaatgat
    16081 gttgtgttaa atcgggaaag cggctcgcaa aaaatgcaca gatttatagg catgataaga
    16141 cgcgtagcgt cgcatcaggc accggtacac aaccgccgga tgcggcgtga acgccttatc
    16201 cggcctacaa aatcgtctct tccgtcaaaa tccgccgcgc gcccaaaaaa tgatcctgcc
    16261 agaaaggttc agctaaccga cttacccgaa tggtttcccc ggtgcgcggt gactcgataa
    16321 actgaccgtc tccaagatat acgcccatat ggtcggctat ctcacggctg tggatatgga
    16381 aaaacagcaa atctccccgg cgcaggtcgt tattcgccac aatcgttgcc cgacggtagt
    16441 ggtacatctc attggccgtg cgcgggagct tagcctcaag gatcttgttg taggcataaa
    16501 aaaccaaccc gctacagtcg aaacctttat caggccgcgt gcccccccag acataaggct
    16561 tgccaagttg ctgttccagg cggtgaatcg ccacttcggt gatgttatgc aggtggttgc
    16621 tgctgaggaa atgattgttt tccgccagcg cctgccagcg tctgtcgttc gccttgagcg
    16681 gcgcaggaaa ccattctgga tgctgtttta tctgctcccg attatgctgg cgcagcgccc
    16741 ttctgctttt agcattgcct tccacaatat agctggcctg ctttttcaga tgggtttggt
    16801 actgttttaa taaacgcgca cggttttgca tccgctggcg ggcggcgtag ctaaaagaga
    16861 tatgtgatgt ggtggcgctg gcgagtaaag ggagcaaaac gcataacgaa aacacgcctg
    16921 gatgcagaaa tctgccgtgc aagaacgaag acatgaagga cataaaccag ctgaatgaag
    16981 gtgagaattt taaaagaccg gaggagtaaa cgggattatt aattgcttat ttgcatatta
    17041 ctattgcggt ttcataatct taggaaataa gaaataaatc tacaatccat gcgcttttcg
    17101 gactcttgaa aactgcacct gtgctaaatt tgtaaaccct gcgccgacag gcttgcgtaa
    17161 aactcttttg gtgataatgt gagccttcca aatccagact attccgattt gggattcatt
    17221 ttgccgtcct ggcgtgtgta accgttttat caaggaataa gcagtatgcg taaaatcgca
    17281 ttaattcttg cgatgttgtt gatcccatgc gtttcgtttg ccggtttgct gggcagcagt
    17341 agctcgacca cgccagtgag caaagagtat aagcagcagt tgatgggatc ccctgtctac
    17401 atccagatct tcaaggaaga acgtacgctc gatctctacg tcaaaatggg cgagcaatat
    17461 caactgctcg acagctataa aatctgtaaa tattccggcg gcttagggcc aaaacagcgt
    17521 cagggcgatt tcaaaagccc ggaagggttt tatagcgtcc agcgtaatca gttaaaacca
    17581 gacagccgtt actacaaagc gattaatatt ggattcccaa acgcctatga ccgtgcgcat
    17641 ggttatgaag ggaaatacct gatgattcac ggcgattgtg tttccatcgg ctgctacgcg
    17701 atgaccaatc agggtattga tgagatcttc cagttcgtta ctggtgcgct ggtgtttggt
    17761 cagccgagcg tgcaagtgag tatttatccg ttccgcatga ccgacgccaa tatgaagcgc
    17821 cataaatatt ccaactttaa ggacttctgg gagcaactga agccgggcta cgactacttt
    17881 gagcaaaccc gtaagccacc gaccgtttct gtggtcaatg gccgttacgt ggtcagcaag
    17941 ccgttaagcc acgaagttgt gcagccacaa ctggcatcaa actacacgct ccccgaggca
    18001 aaataagcgc cattcacctg gcataatctt ttgccaggtt tcattgcccg tcagcggctg
    18061 tgtcgcaatc accgtgacca catcattcgg tgtggtctgc gagctgaagt cgatttccac
    18121 atcctgatcc agcaacgttg ccacgccaaa cggcgcgcgg cgggtgatcc agtgtaaatt
    18181 agtcgagcaa tacgccatta catagcgccc gtcagaaagc agcatgttga aaacgccctt
    18241 ctgccgcagt tcatccgcca gtgaggcgat atatttaaac actgccgcca tgttgcccgg
    18301 tgtgcgcggg taacgctgcg ttaatttatg caggagccag caaaaggctt tttcgctatc
    18361 ggtttcaccg accgggcgga agttgccggt ttccagtgat ttgtagcccg tcagttgtcc
    18421 attatgggca taagtccagt tacgccccca taactcgcgg gtaaacgggt gagtattttc
    18481 cagcgccacc tcgccccgat tagcctggcg aatatgagcc accaccgaac aggattttat
    18541 cgggtagtcc tggacaagtt tggcgatggg ggaattaaag ctgggttgcg gatctttaaa
    18601 tgtgcgacag cctttacctt cgtaaaaggt aatgccccag ccatctttat gtggcccggt
    18661 tccaccacca cgctgtacaa gtccggtgaa actaaagcag atatcggtag ggacgttggc
    18721 actcatcccg agcaattcgc acatgggtaa cctccacaac agcgggagcg catggccccc
    18781 ggcgaagatt gatttaagaa ttttcaggtc ggatgtggcg tgaacgcatt attcggccta
    18841 cggttcgggt gcagattgta ggcatgataa gacgcgtcag cgtcgcatca ggcatcagag
    18901 cacaaatgcc ggatgcggcg taaacgcctt atccggccta cgccagactt acttaaccat
    18961 ctctttttca attaactgga tcaggatatg gatcacttta atgtggatct cctgaatgcg
    19021 gtcggcataa ccaaagtgcg gtacgcgaat ttcgatatcc gccgtgccag ccattttgcc
    19081 gccgtcttta ccggtcaggg tgatcacttt catccccttc tcacgcgccg ccgcgatcgc
    19141 tttgatcacg tttgcagagt taccggaggt ggagatcccc agcagtacat cgccttcgcg
    19201 acctaccgct tcaacgtaac gggagaaaat atcattgaaa ccaaaatcgt taccgacgca
    19261 ggaaatatga ctaacgtcag aaatggcaat cgccgggtag cccggacggt tttcacggta
    19321 gcgaccggtc aactcttcgg caaagtgcat ggcgtcgcaa tgggagccgc cgttgccaca
    19381 ggaaagcact ttgccaccgg ctttaaagct gtctgctaac aggaccgccg cgcgctgaat
    19441 ggcgtgaata ttggcgtcat cttttaaaaa gttagccagc gtttccgccg cttcgttcag
    19501 ttcattacga ataagatcct ggtacatgag gatatccttc agcataaatg taatagacaa
    19561 aatgcagtgt accggatacc gccaaaagcg agaagtacgg gcaggtgcta tgaccaggac
    19621 tttttgacct gaagtgcgga taaaaacagc aacaatgtga gctttgttgt aattatattg
    19681 taaacgcatt gctaaatgtt tttacatcca ctacaaccat atcatcacaa gtggtcagac
    19741 ctcctacaag taaggggctt ttcgttatga tgattttgag tattctcgct acggttgtcc
    19801 tgctcggcgc gttgttctat caccgcgtga gcttatttat cagcagtctg attttgctcg
    19861 cctggacagc cgccctcggc gttgcgggtc tgtggtcggc gtgggtacta gtgcctctgg
    19921 ccattatcct cgtgccattt aactttgcgc ctatgcgtaa gtcgatgatt tccgcaccgg
    19981 tatttcgcgg tttccgtaag gtgatgccgc cgatgtcgcg cactgagaaa gaagcgattg
    20041 atgcgggcac cacctggtgg gagggcgact tgttccaggg caagccggac tggaaaaagc
    20101 tgcataacta tccgcagccg cgcctgaccg ctgaagagca agcgtttctc gacggtccgg
    20161 tagaagaagc ctgccggatg gcgaatgatt tccagattac tcatgagctg gcggacctgc
    20221 cgccggagtt gtgggcgtac cttaaagagc atcgtttctt cgcgatgatc atcaaaaaag
    20281 agtacggcgg gctggagttc tcggcttatg cccagtctcg tgtgctgcaa aaactctccg
    20341 gcgtgagcgg gatcctggca attaccgtcg gcgtgccgaa ctcattaggc ccgggcgagc
    20401 tgctgcaaca ttacggcact gacgagcaga aaaatcacta tctgccgcgt ctggcgcgtg
    20461 gtcaggagat cccttgcttt gcactgacca gccccgaagc gggttccgat gcgggcgcga
    20521 ttccggatac cgggattgtc tgcatgggcg aatggcaggg ccagcaggtg ctggggatgc
    20581 gtctgacctg gaacaaacgc tacattacgc tggcaccgat tgcgaccgtg ctgggtctgg
    20641 cgtttaaact ctccgacccg gagaaattac tcggcggtgc agaagattta ggtattacct
    20701 gtgcgctgat cccgaccacc acgccgggcg tggaaattgg tcgtcgccac ttcccgctga
    20761 acgtaccgtt ccagaacgga ccgacgcgcg gtaaagatgt gttcgtgccg atcgattaca
    20821 tcatcggcgg gccgaaaatg gccgggcaag gctggcggat gctggtggag tgcctctcgg
    20881 taggccgcgg catcaccctg ccttccaact caaccggcgg cgtgaaatcg gtagcgctgg
    20941 caacgggtgc gtatgcgcac attcgccgtc agttcaaaat ttctattggt aagatggaag
    21001 ggattgaaga gccgctggcg cgtattgccg ggaacgccta cgtgatggat gctgcggcat
    21061 cgctgattac ctacggcatt atgctcggtg aaaaacccgc cgtgctgtcg gctatcgtta
    21121 agtatcactg tacccaccgc gggcagcaat cgattatcga cgcaatggat atcaccgggg
    21181 gtaaaggcat tatgctcggg cagagcaact tcctggcccg tgcttaccag ggggcaccga
    21241 ttgccatcac cgttgaaggg gcaaatattc tgacccgtag catgatgatc ttcggtcagg
    21301 gggcgattcg ttgccatccg tacgtgctgg aagagatgga agcggcgaag aacaatgacg
    21361 taaacgcgtt cgataaactg ctgttcaaac atatcggtca cgtcggtagc aacaaagttc
    21421 gcagcttctg gctgggcctg acgcgcggtt taaccagcag cacgccaacc ggcgatgcca
    21481 ctaaacgcta ctatcagcac ctgaaccgcc tgagcgccaa cctcgccctg ctttctgatg
    21541 tctcgatggc ggtgctgggc ggcagcctga aacgtcgtga gcgcatctca gcccgtctgg
    21601 gggatatttt aagccagctt tacctcgcct ctgccgttct gaagcgttat gacgacgaag
    21661 gccgtaatga agccgacctg ccgctggtgc actggggcgt acaggatgcg ctgtatcagg
    21721 cagaacaggc gatggacgat ttactgcaaa acttcccgaa ccgcgtggtt gccgggctgc
    21781 tgaatgtggt gatcttcccg accggacgtc attatctggc accgtctgac aagctggatc
    21841 ataaggtggc gaagatttta caagtgccga atgccacccg ttcacgtatt ggtcgcggtc
    21901 agtacctgac tccaagcgag cataacccgg ttggtttact ggaagaggcg ttggtggatg
    21961 tgattgccgc cgacccgatt catcagcgga tctgtaaaga gctgggtaaa aatctgccat
    22021 ttacccgtct ggatgaactg gcgcacaacg cgctgacgaa ggggctgatt gataaagatg
    22081 aagccgctat tctggtgaaa gctgaagaaa gccgtctgcg tagtattaac gttgatgact
    22141 ttgatccaga agagctggcg acgaagccgg taaagttgcc ggagaaagtg cggaaagttg
    22201 aagccgcgta attgaataac ggagccgaaa ggctccgttt ttttatcggc taattattta
    22261 aaattaaagc catccggatg gttttccagg ctgccggtca acgccgcgaa cagcaccgtt
    22321 ttgccatcaa tcgaaagcgc atcattcaca ttcagccagg tgagtttctc ttgcgacgtt
    22381 ttctcatcaa tagtcgagaa cagccccgtc atctgattag atttctcgga ccacatcaca
    22441 gcgatacgtt gcgagccaca gtcatgaggt ttgcacgcgc tcatcacctg atacgtctca
    22501 tctcccaatg tcacggtttg tgcgggagta taagtaccgc ctttcatcac ccaggcaggc
    22561 agcttatgcc cttgcaccat ctgattaaac gccgctttgg tggtttcgcc ctttgcaaga
    22621 ctgctaatgg ttaaatcatc ctgcgccatt gcactggtgg cgatgaccag agcggcgact
    22681 gtcgttattg ccttaaacat cattcctccc gggcttatcc tgcccatgtg attaactccc
    22741 taaagcctgg cgataacacg ataatttgca aatcagatat ttccaatatc agcatttgtt
    22801 atcaatttat tctgcgctaa ggcaactgcc tgataaggtg ttagagttat aaaacccttc
    22861 gcataaagga gttgtacacc gtgcctggtt tgaagattac gcttttgcag caaccactgg
    22921 tgtggatgga tggtcctgcc aacctgcgcc attttgatcg tcaactggaa ggtattaccg
    22981 ggcgcgatgt gatcgttcta ccggagatgt ttaccagcgg ctttgccatg gaagcggcag
    23041 cttcgtcgct agcacaagat gacgtagtga actggatgac cgctaaagcg cagcagtgca
    23101 atgcgctgat tgcaggcagt gttgcattac aaacggagtc tgattcggtt aaccgctttt
    23161 tgctggttga gccgggcggc acggtacatt tttatgataa gcgtcatctg ttccgcatgg
    23221 cagatgagca tctgcattat aaagcaggca atgagcgagt gattgtggaa tggcgcggct
    23281 ggcgtatttt accgctggtg tgctacgact tacgttttcc ggtgtggtcg cgcaatctca
    23341 acgattatga cctcgccatc tacgttgcca actggcctgc accgcgctct ctgcactggc
    23401 aggcattact gacagcccgc gcgattgaga atcaggcgta tgtagcggga tgtaatcgcg
    23461 tcggcagcga tggcaacggc tgccattatc gcggtgacag ccgggtgatt aatccacaag
    23521 gcgagattat cgctactgcc gacgcgcatc aggcaacgcg cattgatgcg gagctatcga
    23581 tgatggcatt gcgggaatat agagaaaagt ttccggcatg gcaggatgcg gatgagttca
    23641 catttcgtta gagggtgaat atggctacga gataagtttc gtggctattt ttctctggaa
    23701 cttaaacgac gaaatcccga atcagtaatg attcgggctt tctgaatatt ggcggaacgg
    23761 acgggactcg aacccgcgac cccctgcgtg acaggcaggt attctaaccg actgaactac
    23821 cgctccgcgt tgtgttccgt tgggaacgag gcgaatatta cggattgcct cacccttcgt
    23881 caacggtttt tctcatcttt tgaatcgttt gctgcaaaaa tcgcccaagt cgctattttt
    23941 agcgcctttt acaggtattt atgcccgcca gaggcaggtt ccgcccttct tctgcaccag
    24001 atcaagacgg gcttcatgtg ctgcaagctc ttcatctgtc gcaaaaacaa cgcgtaactt
    24061 acttgcctga cgcacaatgc gctgaattgt tgtttcacct tgttgctgtt gtgtctctcc
    24121 ttccatcgca aaagccatcg acgtttgacc accggtcatc gccagataaa cttccgcaag
    24181 gatctgggca tcgagtaatg ccccatgcag cgttcgttta ctgttatcta tttcgtagcg
    24241 ggcacataac gcatcgaggc tgttgcgttt accgggaaac attttcctcg ccaccgcaag
    24301 gctatcggtg accttacaga aggtattggt cttcggaata tcgcgcttaa gcaacgaaaa
    24361 ctcgtagtcc ataaagccga tatcaaacgc tgcgttatgg atcaccaact ccgcgccgcg
    24421 gatatagtcc atgaactcat cggctacctc ggcaaacgtg ggcttatcga gcaaaaattc
    24481 atcggcaata ccatgtacgc caaaggcttc cggatccacc agccgatcgg gtttgagata
    24541 aacatggaag ttattgcccg tcaggcgacg gttcaccact tcaacggcac caatctcaat
    24601 gatcttgtgg ccttcatagt gcgcaccaat ctggttcata ccggtggttt cagtatcgag
    24661 aacgatctgg cgtgtaattg cagtgctcat agcggtcatt tatgtcagac ttgtcgtttt
    24721 acagttcgat tcaattacag gaagtctacc agagatgctt aaacaggtag aaattttcac
    24781 cgatggttcg tgtctgggta atcctggacc tgggggttac ggcgctattt tacgctatcg
    24841 cggacgcgag aaaaccttta gcgctggcta cacccgcacc accaacaacc gtatggagtt
    24901 gatggccgct attgtcgcgc tggaggcgtt aaaagaacat tgcaaagtca ttttgagtac
    24961 cgacagccag tatgtccgcc agggtatcac ccagtggatc cataactgga aaaaacgtgg
    25021 ctggaaaaca gcagacaaaa aaccagtaaa aaatgtcgat ctctggcaac gtcttgatgc
    25081 tgctctgggg cagcatcaaa tcaaatggga atgggttaaa ggccatgccg gacacccgga
    25141 aaacgaacgc tgtgatgaac tggctcgtgc tgcggcaatg aatcccacac tggaagatac
    25201 aggctatcaa gtcgaagttt aagcctgtgg tttacgacat tgccgggtgg caccaaccgc
    25261 ctggcgaatt cgcggtttat tcttgctctg tttcatcgga tttagcgtta aaggaatagt
    25321 ccgtttccgg gcaacaataa gttgtaagca accaagcgca ggaatatgcg cattcaatag
    25381 ttttcctccg tgtttgttcc acgggagaac gtggaaacgg ctggcgtgta gcacttcaaa
    25441 attcaacaaa gagagccagt ccagctgccg catcagagta aacatccggc tgttataggg
    25501 cgatgttttg cgcaataccg gcacaagttt gcgtaatccc attaaactga tgggattgaa
    25561 gccactaatc accagccaac catcatcaat cagtacccga tcggcttcac gcaataaacg
    25621 atgcggatca gtgcaccacg gcaatgtatg tgccagtaga caaacatcaa cggatttatc
    25681 ggcaaaagga agatgaagtg ggtccgcctg cacctggacg ggcattcctt gcgcagaaac
    25741 attcacttga tgagaaaccg cgcacgcttc gcaattgatt tctgcgctta aattgccaat
    25801 cttaagcaga tgaaaaccat acattttagt gaaccacggg ttgagctgac gctccagcgc
    25861 cttgcgataa agctctcccc agggcaaatc gccccagcaa tcaggagcca cgacagtttg
    25921 agggactctt gccggtttca tcacaacctt ccgtttcaca ctgagaggta atctatgaat
    25981 cttaacagta ttcccgcctt tgatgacaat tacatctggg ttttgaatga tgaagcaggt
    26041 cgctgtctga ttgtcgatcc cggggacgca gagccagtat taaacgccat ttccgccaat
    26101 aactggcaac cggaggccat atttctcacc caccatcacc acgatcacgt tggcggcgta
    26161 aaagaactgg tggaaaagtt tccgcaaatt gtggtgtatg gaccacaaga gacacaagat
    26221 aagggaacaa cacaggtagt caaagatggc gaaactgcct tcgttttggg gcatgaattt
    26281 agtgtaattg ccacaccggg tcacacttta ggacatatct gttacttcag taaaccttat
    26341 ctattttgcg gcgacacgct gttttctggt gggtgtggtc ggttgtttga agggacacca
    26401 tcacaaatgt atcaatcact tagaaagtta agtgcgttac ctgacgacac attggtatgt
    26461 tgtgctcatg aatatacctt atcaaatatg aagtttgctt tgagtattct tccgcacgat
    26521 ttgtccataa atgattatta tcgtaaagtt aaggagttac gggcaaaaaa tcaaataaca
    26581 ctacccgtaa ttctgaaaaa tgagcggcaa attaatgttt ttttaagaac ggaagatatt
    26641 gatttaatta atgtaattaa tgaagaaaca ttattgcaac aacctgaaga acgttttgca
    26701 tggttaaggt caaagaaaga taggttctga taaaactttc ttgtcatcgg ctccgttcgc
    26761 cgttatgatc ggtcgtcttt taagcaacta ttgacacaca catgaaggca aaagcgatat
    26821 tactcgcctc tgtcctgctc gtgggttgcc agagcaccgg caacgttcaa cagcacgcac
    26881 agagcctttc tgcagctggt caaggggaag cagcaaagtt tacaagtcag gcacgatgga
    26941 tggacgatgg gacgtctatc gcgccagatg gtgacttgtg ggctttcatt ggcgacgagc
    27001 taaagatggg aattccggaa aacgaccgga ttcgcgaaca gaaacagaaa tatttacgca
    27061 ataagagcta tctccacgat gtaactttac gggcagagcc gtatatgtac tggatagccg
    27121 ggcaagttaa aaaacgtaac atgcctatgg aactggtact actacccata gtggagagcg
    27181 cttttgatcc tcacgcaacg tctggcgcca atgccgcggg catctggcag atcattccga
    27241 gcacggggcg caattatggt ttgaaacaga cccgcaatta tgacgcgcgt cgcgatgttg
    27301 ttgcttcaac aactgccgcg ctgaacatga tgcagcgtct gaacaagatg tttgacggcg
    27361 actggcttct gaccgtagcg gcttataaca gcggcgaagg tcgggtcatg aaggcaatta
    27421 aaacgaacaa agcgcgtggg aaatccacgg acttctggtc gttaccgttg ccgcaggaaa
    27481 cgaagcagta cgtgcctaaa atgctggcat tgagtgatat tctcaaaaac agcaagcgtt
    27541 atggcgtacg tctgccaacg accgatgaaa gccgtgctct ggcacgtgtg cacctgagca
    27601 gcccggttga aatggcgaag gttgcagata tggcggggat ttccgtcagc aagctgaaga
    27661 cattcaacgc aggcgtgaaa ggctccacgc tgggcgcaag tggtccgcag tacgtgatgg
    27721 tgccaaagaa gcatgcagat caactgcgtg aatctctggc ttcaggcgaa attgctgctg
    27781 tacagtcgac gctggttgcc gacaatacgc cacttaacag ccgtgtttac accgtacgct
    27841 ctggcgacac gctttcaagt atcgcttcac gtctcggcgt aagcaccaaa gatttgcagc
    27901 agtggaacaa actgcgcgga tctaagctga agccaggcca aagtttgacg attggtgcag
    27961 gcagtagcgc acagcgactg gcaaacaaca gcgatagcat tacgtatcgt gtgcgcaaag
    28021 gcgattcgct ttcaagcatt gctaaacgcc acggcgtgaa catcaaagat gtaatgcgct
    28081 ggaacagcga cactgcgaat ctgcaaccag gcgataagct gacgttgttt gtgaaaaaca
    28141 acaacatgcc agattcctga caaaccagat aataaaaaag gcaccgattc ccccggtgcc
    28201 ttttttattt atgccgcttt atgtgcttct agcatgatga tatcactggt gaaagagcca
    28261 tcattctgca aggcaaaata cgttttcacc tctgtcgatg cgctctgttg gtaaatgcga
    28321 atagcgtcta ctaacgcttc tggcgtacgc attctcgcta cccatgaaga aaattccagc
    28381 ggtaacttat ctgtaattaa attatcaact atcagattgg cttcactgat taacgtcaac
    28441 cactcaccgc tggcgtggtt ccgtatgtga gatgtatcgc gtaatgcttc taccgtctgt
    28501 aaccagatgt cgcgcactgg gtgacccgga gacattacgt ccatcacaat cagcagacca
    28561 ccgggtttca atacccggtt cacttcacgc aatgctgcac caacatcatg ccaatgatgg
    28621 gcagaataac ggctgataac aatatcaaat gcgttatcgg caaatggcag gctttcgaca
    28681 taaccctggc gggtggtgat atttttcagt tgccgggctt cggcagcttg tgccacgaca
    28741 tccagcatat gggcagataa gtcatacgcc accaccgtgc tcacgttttg cgcagcgaca
    28801 aagctggcat gccctgctcc gcagcccata tcaagtacac ttgcatcagg ataatcagcc
    28861 agacgcaccg ccagacgctg taaatcttgt ccggatgcat gtacggtact ggtcagatat
    28921 tcgctggctt gagagctaaa ctgcttttct acgtggtcat ggtgggattg tgttgtcatt
    28981 gcgctgtcct tttgttattt gagtataaaa gggcagcgcc aaacgttggt ctgccctgtg
    29041 gcagacctga cataccttat ttatcaggct tgccgggact gaattcaacg agtagcggat
    29101 tgtgatcgga cgcgcgcgta accagtacag aagcttcgct gacgttcaga ccacggtaga
    29161 aaacaaaatc gagcgggcga ccaaacgccc gacggcgctg atcatcggta aaacgcacct
    29221 ggcgcagcga catttcccgc gcaaagcgat ataacgcgtt catccttcta cggctccagg
    29281 cattgaaatc tcccgccata atgaccgggc cactgtggtg agctatctga tcgccaatag
    29341 gaagtaactg cttactatag acatccacgc ccagactgaa gttgacggcg tgtatattaa
    29401 ccaccatcaa caggcgggtg tcaggtaacg gatagaccgt caccagtgcc gacttcgcca
    29461 gacgcaaaat gggttctcgt tcacgtaacg ggcagcaata cactggatgt gccgccgaaa
    29521 gggtcattac gccagaagga tgttgtggca gcacgaaagc gggtacctga tcggcggcaa
    29581 gatagttagc agtcgcaaac tgtactaact ctggcgttgt ctgcgcttcc tgcaataaca
    29641 ccagatgtgc atctttgccg tagttcttta ataccgacaa ccattcagcg cgttgctgtt
    29701 tgtatatgtt ccacaccagg atccgaatac gctcttcggt acttaacggt tctccaggtg
    29761 gtaatgcctg gccgatgctc gcaaaagacc ccggcggtaa gatcctttcc gcaggttgtc
    29821 cggcaacata gcgcatggca taggtgtttt ttcgcacttt tgactttgaa acctctatca
    29881 cgtcaaccct tccgcactcg ggagggttct actgttatag ggattttagc tcactctttc
    29941 aacgagcaat tgtatattgt tatgtaagca agtgcttaca ccggtcagga ttttgctcat
    30001 tattgagaac attaaatcgg atggcatatc tcgcatcctg atggtttaat gacaaataaa
    30061 ttcaaaatat ctcttctatt ttgcaacagg agcaaaaatg aaagccacgt cggaagaact
    30121 cgccattttt gtttcagtcg tcgaaagcgg cagctttagc cgggcagcgg aacaattagg
    30181 gcaagcaaac tcagcggtaa gccgggcggt gaaaaagctg gagatgaaac ttggcgttag
    30241 cctgcttaat cggaccacgc ggcaacttag cctgacggaa gaaggcgagc gttattttcg
    30301 tcgcgtacag tcaattttgc aggagatggc agcggcagaa tcagaaatta tggagacgcg
    30361 taatacaccg cgtggactgt tacggatcga tgccgcaact ccagtggtgc tgcactttct
    30421 gatgccgttg attaagcctt ttcgtgaacg ctatccggaa gtcactttat cgctagtctc
    30481 ctccgaaacg attattaatt tgatcgaaag aaaagtggat gtcgcgatac gcgccggtac
    30541 gttaacggat tccagcttac gtgccaggcc gctatttaac agttatcgaa aaattatcgc
    30601 ctcccccgat tatatttccc gctacgggaa gccagaaacg atcgacgatt taaagcaaca
    30661 tatttgcctg ggattcactg aacccgcttc cctcaatacc tggccgatag cctgtagcga
    30721 tggacaatta catgaggtta agtacggttt gtcatccaat agtggggaaa cactgaaaca
    30781 gctttgcctg agtggcaacg ggattgcgtg tttgtccgac tacatgatcg acaaagaaat
    30841 cgctcgcgga gaattggtgg agttaatagc agataaagtg ttgccagtag aaatgccatt
    30901 cagtgcagtc tattacagcg accgtgcggt aagtacgcgc atccgggctt ttatcgattt
    30961 ccttagcgag catgtaaaaa cagctcccgg aggagctgtc agagaggctt aatcccattc
    31021 aggagccaga ccttccgggc taaccaggcg gtcgttgcaa tccagtgcgg cgatcgcttt
    31081 tttatcttcg gcatcaagct gtaaattttg tgccttaaga ttactctcca ggttttcacg
    31141 tttagtagaa gaaggaatta ctgaataacc ttcccccata gcccacgcca gaatcacttg
    31201 agccggagtc gcattgtgtt tagctgcaat acgagcaata acctcatctt tcagtgcctt
    31261 gccatacgcc agcgtcatat aggaagtaat atggatactg tgctgtttag cccaagcgac
    31321 cactttacgg ttttgcagat aaggagagag ttcaatctgg ttagtagcga tgttttcagc
    31381 gccaacagca gcaatcgctt tttccatcaa tgggatcgta aagttggaaa taccgatctc
    31441 acgcgtcagc ccttgttttt tggcttccag cagcgcctgc ataaactctt caacagagac
    31501 ttcatcgttt ggtgacggcc agtggattag cgtcagatca acataatcgg tacgcaattt
    31561 ttgcaggctc tctttcagac tcgggatcaa tttgtctttg ctgagatttt caatccagat
    31621 tttagtggtg atgtggagtt catgacgtgg cacgccactt tctgcaatcg cctgacctac
    31681 tgcggcttcg ttatcataga tttgtgcggt atcaattgcg cgataatcaa gttcaagcgc
    31741 cgttttcaca gatgaaataa caacgtcgtc tttcagacgg aaagtgccta aaccaaatgc
    31801 agggatagcc ataatatgcc tcttttaatg ctattttcgt taactgaggt gattatgggc
    31861 ttcagactgt tgcggaaaaa tggcgtaaaa cgcagaggat ttttgcgatt ttggcaataa
    31921 tagaaataca aacgaaaaaa aacctcgagc gttaactcga ggcttcttag taagtggcgg
    31981 aacggacggg actcgaaccc gcgaccccct gcgtgacagg caggtattct aaccgactga
    32041 actaccgctc caccgaattc ttttacaacc accggtttta tgaccggctt actgcttaat
    32101 ttgat
//
LOCUS       DAHHTG010000048        31779 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.048, whole genome shotgun sequence.
ACCESSION   DAHHTG010000048 DAHHTG010000000
VERSION     DAHHTG010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 31779)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31779)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31779
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.048"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            <1..611
                     /gene="lpxT"
                     /locus_tag="NBI74_002145"
     CDS             <1..611
                     /gene="lpxT"
                     /locus_tag="NBI74_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Kdo(2)-lipid A phosphotransferase"
                     /protein_id="HCD1932480.1"
                     /translation="ADIFYFFNQKLVESKAFLWLVALTNNRAFDGCSLLAMGMLMLSF
                     WLKENAPGRRRIVIIGLVMLLTAVVLNQLGQALIPVKRASPTLTFTNINRVSELLSVP
                     TKDASRDSFPGDHGMMLLIFSAFMWRYFGKVAGLIALIIFVVFAFPRVMIGAHWFTDI
                     IVGSMTVILIGLPWVLLTPLSDRLITFFDKSLPGKNKHFQNK"
     gene            1023..1589
                     /gene="mepS"
                     /locus_tag="NBI74_002146"
     CDS             1023..1589
                     /gene="mepS"
                     /locus_tag="NBI74_002146"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional murein DD-endopeptidase/murein
                     LD-carboxypeptidase"
                     /protein_id="HCD1932481.1"
                     /translation="MVKSQPILRYILRGIPAIAVAVLLSACSANNTAKNMHPETRAVG
                     SETSSLQASQDEFENLVRNVDVKSRIMDQYADWKGVRYRLGGSTKKGIDCSGFVQRTF
                     REQFGLELPRSTYEQQEMGKSVSRSNLRTGDLVLFRAGSTGRHVGIYIGNNQFVHAST
                     SSGVIISSMNEPYWKKRYNEARRVLSRS"
     gene            1770..3326
                     /locus_tag="NBI74_002147"
     CDS             1770..3326
                     /locus_tag="NBI74_002147"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
                     /protein_id="HCD1932482.1"
                     /translation="MFIRAPNSGRKLLLTCIVAGVMIAILVSCLQFLVAWHKHEVKYD
                     TLITDVQKYLDTYFADLKSTTDRLQPLTLDTCQQANPELTARAAFSMNVRTFVLVKDK
                     KTFCSSATGEMDIPLNELIPALDINKNVDMAILPGTPMVPNKPAIVIWYRNPLLKNSG
                     VFAALNLNLTPSLFYSSRQEDYDGLALIIGNTALSTFSSRLMNVNELTDMPVRETKIA
                     GIPLTVRLYADDWTWNDVWYAFLLGGMSGTFVGLLCYYLMSVRMRPGREIMTAIKREQ
                     FYVVYQPVVDTQALRVTGLEVLLRWRHPVAGEIPPDAFINFAEAQKMIVPLTQHLFEL
                     IARDAAELEKVLPVGVKFGINIAPAHLHSESFKADIQKLLTSLPAHHFQIVLEITERD
                     MLKEREATQLFAWLHSVGVEIAIDDFGTGHSALIYLERFTLDYLKIDRGFINAIGTET
                     ITSPVLDAVLTLAKRLNMLTVAEGVETPEQARWLSERGVNFMQGYWISRPLPLDDFVR
                     WLKKPYTPQW"
     gene            3408..5222
                     /locus_tag="NBI74_002148"
     CDS             3408..5222
                     /locus_tag="NBI74_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311096.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCD1932483.1"
                     /translation="MIVRILLLFIALFTFGAQAQAIKESYAFAVLGEPRYAFNFNHFD
                     YVNPAAPKGGQITLSALGTFDNFNRYALRGNPGARTEQLYDTLFTTSDDEPGSYYPLI
                     AESARYADDYSWVEVAINPRARFHDGSPITARDVEFTFQKFMTEGVPQFRLVYKGTTV
                     KAIAPLTVRIELAKPGKEDMLSLFSLPVFPEKYWKDHKLSDPLATPPLASGPYRITSW
                     KMGQNIVYSRVKDYWAANLPVNRGRWNFDTIRYDYYLDDNVAFEAFKAGAFDLRMEND
                     AKNWATRYTGKNFDKKYIIKDEQKNESAQDTRWLAFNIQRPVFSDRRVREAITLAFDF
                     EWMNKALFYNTWSRTNSYFQNTEYAARNYPDAAELVLLAPMKKDLPPEVFTQIYQPPV
                     SKGDGYDRDNLLKADKLLNEAGWVLKGQQRVNATTGQPLSFELLLPASSNSQWVLPFQ
                     HSLQRLGINMDIRKVDNSQITNRMRSRDYDMMPRVWRAMPWPSSDLQISWSSEYINST
                     YNAPGVQSPVIDSLINQIIAAQGNKEKLLPLGRALDRVLTWNYYMLPMWYMAEDRLAW
                     WDKFSQPAVRPVYSLGIDTWWYDVNKATKLPSARQQGE"
     gene            5223..6317
                     /locus_tag="NBI74_002149"
     CDS             5223..6317
                     /locus_tag="NBI74_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter permease YejB"
                     /protein_id="HCD1932484.1"
                     /translation="MGAYLIRRLLLVIPTLWAIITINFFIVQIAPGGPVDQAIAAIEF
                     GNAGVLPGAGGEGVRASHAQTGVGNISDSNYRGGRGLDPEVIAEITHRYGFDKPIHER
                     YFKMLWDYIRFDFGDSLFRSASVLTLIKDSLPVSITLGLWSTLIIYLVSIPLGIRKAV
                     YNGSSFDVWSSAFIIIGYAIPAFLFAILLIVFFAGGSYFDLFPLRGLVSANFDSLPWY
                     QKITDYLWHITLPVLATVIGGFAALTMLTKNSFLDEVRKQYVVTARAKGVSEKNILWK
                     HVFRNAMLLVIAGFPATFISMFFTGSLLIEVMFSLNGLGLLGYEATVSRDYPVMFGTL
                     YIFTLIGLLLNIVSDISYTLVDPRIDFEGR"
     gene            6317..7342
                     /locus_tag="NBI74_002150"
     CDS             6317..7342
                     /locus_tag="NBI74_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCD1932485.1"
                     /translation="MSRLSPVNQARWARFRHNRRGYWSLWIFLLLFGLSLCSELIAND
                     KPLLVRYDGSWYFPLLKNYSESDFGGPLASQADYQDPWLKQRLENNGWVLWAPIRFGA
                     TSINFATDKPFPSPPSRQNWLGTDANGGDVLARILYGTRISVLFGLMLTLCSSVMGVL
                     AGALQGYYGGKVDLWGQRFIEVWSGMPTLFLIILLSSVVQPNFWWLLAITVLFGWMSL
                     VGVVRAEFLRTRNFDYIRAAQALGVSDRSIILRHMLPNAMVATLTFLPFILCSSITTL
                     TSLDFLGFGLPLGSPSLGELLLQGKNNLQAPWLGITAFLSVAILLSLLIFIGEAVRDA
                     FDPNKAV"
     gene            7344..8933
                     /gene="yejF"
                     /locus_tag="NBI74_002151"
     CDS             7344..8933
                     /gene="yejF"
                     /locus_tag="NBI74_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter ATP-binding protein
                     YejF"
                     /protein_id="HCD1932486.1"
                     /translation="MTQTLLAIENLSVGFRHQQTVRTVVNDVSLHIEAGETLALVGES
                     GSGKSVTALSILRLLPCPPVEYLSGDIRFHGESLLHASDQTLRGVRGNKIAMIFQEPM
                     VSLNPLHTLEKQLYEVLSLHRGMRREAARGEILNCLDRVGIRQAAKRLTDYPHQLSGG
                     ERQRVMIAMALLTRPELLIADEPTTALDVSVQAQILQLLRELQGELNMGMLFITHNLS
                     IVRKLAHRVAVMQNGRCVEKNNAATLFASPTHPYTQKLLNSEPSGDPVPLPEPASTLL
                     DVEQLQVAFPIRKGILKRIVDHNVVVKNISFTLRAGETLGLVGESGSGKSTTGLALLR
                     LINSHGSIVFDGQPLQNLNRRQLLPIRHRIQVVFQDPNSSLNPRLNVLQIIEEGLRVH
                     QPTLSAAQREQQVIAVMHEVGLDPETRHRYPAEFSGGQRQRIAIARALILKPSLIILD
                     EPTSSLDKTVQAQILTLLKSLQQKHQLAYLFISHDLHVVRALCHQVIVLRQGEVVEQG
                     PCARVFAAPQQEYTRQLLALS"
     gene            complement(8937..9281)
                     /locus_tag="NBI74_002152"
     CDS             complement(8937..9281)
                     /locus_tag="NBI74_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1932487.1"
                     /translation="MTSLQLSIVHRLPQNYRWSAGFAGSKVEPIPQNGPCGDNSLVAL
                     KLLSPDGDNAWSVMYKLSQALSDIEVPCSVLECEGEPCLFVNRQDEFAATCRLKNFGV
                     AIAEPFSNYNPF"
     gene            complement(9615..10805)
                     /gene="bcr"
                     /locus_tag="NBI74_002153"
     CDS             complement(9615..10805)
                     /gene="bcr"
                     /locus_tag="NBI74_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter Bcr"
                     /protein_id="HCD1932488.1"
                     /translation="MTTRQHSSFAIVFILGLLAMLMPLSIDMYLPALPVISAQFGVPA
                     GSTQMTLSTYILGFALGQLIYGPMADSFGRKPVVLGGTLVFAAAAVACALAQTIDQLI
                     VMRFFHGLAAAAASVVINALMRDIYPKEEFSRMMSFVMLVTTIAPLMAPIVGGWVLVW
                     LSWHYIFWILAVAAILASAMIFFLIKETLPPERRQPFHIRTTIGNFAALFRHKRVLSY
                     MLASGFSFAGMFSFLSAGPFVYIEINHVAPENFGYYFALNILFLFVMTIFNSRFVRRI
                     GALNMFRSGLWIQFIMAAWMVISALLGLGFWSLVVGVAAFVGCVSMVSSNAMAVILDE
                     FPHMAGTASSLAGTFRFGIGAIVGALLSLATFNSAWPMIWSIAFCATSSILFCLYASR
                     PKKR"
     gene            complement(10833..11528)
                     /gene="rsuA"
                     /locus_tag="NBI74_002154"
     CDS             complement(10833..11528)
                     /gene="rsuA"
                     /locus_tag="NBI74_002154"
                     /EC_number="5.4.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase RsuA"
                     /protein_id="HCD1932489.1"
                     /translation="MRLDKFIAQQLGVSRAIAGREIRGNRVTVDGEIVRNAAFKLLPE
                     HDVAYDGNPLAQQHGPRYFMLNKPQGYVCSTDDPDHPTVLYFLDEPVAWKLHAAGRLD
                     IDTTGLVLMTDDGQWSHRITSPRHHCEKTYLVTLESPVADDTAEQFAKGVQLHNEKDL
                     TKPAVLEVITPTQVRLTISEGRYHQVKRMFAAVGNHVVELHRERIGGITLDADLAPGE
                     YRPLTEEEIASVV"
     gene            11678..13438
                     /locus_tag="NBI74_002155"
     CDS             11678..13438
                     /locus_tag="NBI74_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HCD1932490.1"
                     /translation="MIFTLRPYQQEAVDATLNHFRRHKTPAVIVLPTGAGKSLVIAEL
                     ARLARGRVLVLAHVKELVAQNHAKYQALGLEADIFAAGLKRKESHGKVVFGSVQSVAR
                     NLDAFQGEFSLLIVDECHRIGDDEESQYQQILTHLTKVNPHLRLLGLTATPFRLGKGW
                     IYQFHYHGMVRGDEKALFRDCIYELPLRYMIKHGYLTPPERLDMPVVQYDFSRLQAQS
                     NGLFSEADLNRELKKQQRITPHIISQIMEFAATRKGVMIFAATVEHAKEIVGLLPAED
                     AALITGDTPGSERDVLIDDFKAQRFRYLVNVAVLTTGFDAPHVDLIAILRPTESVSLY
                     QQIVGRGLRLAPGKTDCLILDYAGNPHDLYAPEVGTPKGKSDNVPVQVFCPACGFANT
                     FWGKTTADGTLIEHFGRRCQGWFEDDDGHREQCDFRFRFKNCPQCNAENDIAARRCRE
                     CDTILVDPDDMLKAALRLKDALVLRCSGMSLQHGHDEKGEWLKITYYDEDGADVSERF
                     RLQTPAQRTAFEQLFIRPHTRTPGIPLRWITAADVLAQQALLRHPDFVVARMKGQYWQ
                     VREKVFDYEGRFRRAHELRG"
     gene            13563..13847
                     /gene="rplY"
                     /locus_tag="NBI74_002156"
     CDS             13563..13847
                     /gene="rplY"
                     /locus_tag="NBI74_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000494192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HCD1932491.1"
                     /translation="MFTINAEVRKEQGKGASRRLRAANKFPAIIYGGKEAPLAVELDH
                     DKVMNMQVKAEFYSEVLTIVVDGKEIKVKAQDVQRHPYKPKLLHIDFVRA"
     gene            complement(13986..14993)
                     /gene="yejK"
                     /locus_tag="NBI74_002157"
     CDS             complement(13986..14993)
                     /gene="yejK"
                     /locus_tag="NBI74_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein YejK"
                     /protein_id="HCD1932492.1"
                     /translation="MSLDINQIALHQLIKRDEQNLELVLRDSLLEPTETVVEMVAELH
                     RVYSAKNKAYGLFSEESELAQTLRLQRQGEEDFLAFSRAATGRLRDELAKYPFADGGF
                     VLFCHYRYLAVEYLLVAVLSNLSSMRVNENLDINPTHYLDINHADIVARIDLTEWETN
                     PESTRYLTFLKGRVGRKVADFFMDFLGASEGLNAKAQNRGLLQAVDDFTAEAQLDKAE
                     RQNVRQQVYSYCNEQLQAGEEIELESLSKELAGVSEVSFTEFAAEKGYELEESFPADR
                     STLRQLTKFAGSGGGLTINFDAMLLGERIFWDPATDTLTIKGTPPNLRDQLQRRTSGG
                     N"
     gene            15175..15402
                     /locus_tag="NBI74_002158"
     CDS             15175..15402
                     /locus_tag="NBI74_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YejL family protein"
                     /protein_id="HCD1932493.1"
                     /translation="MPQISRYSDEQVEQLLAELLNILEKHKAPTDLSLMVLGNMVTNL
                     INTSIAPAQRQAIANSFARALQSSINEDKAH"
     gene            15422..17182
                     /gene="pbgA"
                     /locus_tag="NBI74_002159"
     CDS             15422..17182
                     /gene="pbgA"
                     /locus_tag="NBI74_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin transport protein PbgA"
                     /protein_id="HCD1932494.1"
                     /translation="MVTHRQRYREKVSQMVSWGHWFALFNILLSLVIGSRYLFIADWP
                     TTLAGRIYSYVSIIGHFSFLVFATYLLILFPLTFIVGSQRLMRFLSVILATAGMTLLL
                     IDSEVFTRFHLHLNPIVWQLVINPDENEMARDWQLMFISVPVILLLELVFATWSWQKL
                     RSLTRRRRFARPLAAFLFIAFIASHVVYIWADANFYRPITMQRANLPLSYPMTARRFL
                     EKHGLLDAQEYQRRLIEQGNPDAVSVQYPLSELRYRDMGTGQNVLLITVDGLNYSRFE
                     KQMPALAGFAEQNISFTRHMSSGNTTDNGIFGLFYGISPSYMDGILSTRTPAALITAL
                     NQQGYQLGLFSSDGFTSPLYRQALLSDFSMPSVRTQSDEQTATQWINWLGRYAQEDNR
                     WFSWVSFNGTNIDDSNQQAFARKYSRAAGNVDDQINRVLNALRDSGKLDNTVVIITAG
                     RGIPLSEEEETFDWSHGHLQVPLVIHWPGTPAQRINALTDHTDLMTTLMQRLLHVSTP
                     ASEYSQGQDLFNPQRRHYWVTAADNDTLAITTPKKTLVLNNNGKYRTYNLRGERVKDE
                     KPQLSLLLQVLTDEKRFIAN"
     gene            17257..17333
                     /locus_tag="NBI74_002160"
     tRNA            17257..17333
                     /locus_tag="NBI74_002160"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:17291..17293,aa:Pro,seq:ggg)
     gene            complement(17443..18387)
                     /locus_tag="NBI74_002161"
                     /pseudo
     CDS             complement(17443..18387)
                     /locus_tag="NBI74_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247409.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
     gene            18707..19354
                     /gene="narP"
                     /locus_tag="NBI74_002162"
     CDS             18707..19354
                     /gene="narP"
                     /locus_tag="NBI74_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite response regulator protein NarP"
                     /protein_id="HCD1932495.1"
                     /translation="MPEATPFQVMIVDDHPLMRRGVRQLLELDSGFEVVAEAGDGASA
                     IDLANRLDIDVILLDLNMKGMSGLDTLNALRRDGVTAQIIILTVSDASSDVFALIDAG
                     ADGYLLKDSDPEVLLEAIRAGAKGSKVFSERVNQYLREREMFGAEEDPFSVLTERELD
                     VLHELAQGLSNKQIASVLNISEQTVKVHIRNLLRKLNVRSRVAATILFLQQRGAQ"
     gene            complement(19389..20441)
                     /gene="ccmH"
                     /locus_tag="NBI74_002163"
     CDS             complement(19389..20441)
                     /gene="ccmH"
                     /locus_tag="NBI74_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis thiol:disulfide
                     oxidoreductase CcmH"
                     /protein_id="HCD1932496.1"
                     /translation="MRFLLGVLMLMISGSALATIDVLQFKDEAQEQQFRQLTEELRCP
                     KCQNNSIADSNSMIATDLRQKVYELMQEGKSKKEIVDYMVARYGNFVTYDPPLTPLTV
                     LLWVLPVVAIGIGGWVIYARSRRRVRVVPEAFPEQSVPEGKRAGCVVYLPGIVVALIV
                     AGVSYYQTGNYQQVKIWQQATAQAPALLDRALDPKADPLNEEEMSRLALGMRTQLQKN
                     PGDIEGWIMLGRVGMALGNASIATDAYATAYRLDPKNSDAALGYAEALTRSSDPNDNR
                     LGGELLRQLVRTDHSNIRVLSMYAFNAFEQQRFGEAVAAWEMMLKLLPANDTRRAVIE
                     RSIAQAMQHLSPQESK"
     gene            complement(20438..20995)
                     /gene="dsbE"
                     /locus_tag="NBI74_002164"
     CDS             complement(20438..20995)
                     /gene="dsbE"
                     /locus_tag="NBI74_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbE"
                     /protein_id="HCD1932497.1"
                     /translation="MKRKVLLIPLIIFLAIAAALLWQLARNAEGDDPTNLESALIGKP
                     VPKFRLESLDNPGQFYQADVLTQGKPVLLNVWATWCPTCRAEHQYLNQLSAQGIRVVG
                     MNYKDDRQKAISWLKELGNPYALSLFDGDGMLGLDLGVYGAPETFLIDGNGIIRYRHA
                     GDLNPRVWEEEIKPLWEKYSKEAAQ"
     gene            complement(20992..22935)
                     /gene="ccmF"
                     /locus_tag="NBI74_002165"
     CDS             complement(20992..22935)
                     /gene="ccmF"
                     /locus_tag="NBI74_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000982423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis heme lyase CcmF"
                     /protein_id="HCD1932498.1"
                     /translation="MMPEIGNGLLCLALGIALLLSVYPLWGVARGDARMMASSRLFAW
                     LLFMSVAGAFLVLVNAFVVNDFTVTYVASNSNTQLPVWYRVAATWGAHEGSLLLWVLL
                     MSGWTFAVAIFSQRIPLDIVARVLAIMGMVSVGFLLFILFTSNPFSRTLPNFPIEGRD
                     LNPLLQDPGLIFHPPLLYMGYVGFSVAFAFAIASLLSRRLDSTYARFTRPWTLAAWIF
                     LTLGIVLGSAWAYYELGWGGWWFWDPVENASFMPWLVGTALMHSLAVTEQRASFKAWT
                     LLLAISAFSLCLLGTFLVRSGVLVSVHAFASDPARGMFILAFMVLVIGGSLLLFAARG
                     HKVRSRVNNALWSRESLLLANNVLLVAAMLVVLLGTLLPLVHKQLGLGSISIGEPFFN
                     TMFTWLMVPFALLLGVGPLVRWGRDRPRKIRNLLIIAFISTLVLSLLLPWLFESKVVA
                     MTVLGLAMACWIAVLAIAEAALRISRGTKTTFSYWGMVAAHLGLAVTIVGIAFSQNYS
                     VERDVRMKSGDSVDIHEYRFTFRDVKEVTGPNWRGGVATIGVTRDGKPETVLYAEKRY
                     YNTAGSMMTEAAIDGGITRDLYAALGEELENGAWAVRLYYKPFVRWIWAGGLMMALGG
                     LLCLFDPRYRKRVNPQKTAPEAA"
     gene            complement(22932..23411)
                     /gene="ccmE"
                     /locus_tag="NBI74_002166"
     CDS             complement(22932..23411)
                     /gene="ccmE"
                     /locus_tag="NBI74_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HCD1932499.1"
                     /translation="MNIRRKNRLWIACAVLAGLALTIGLVLYALRSNIDLFYTPGEIL
                     YGKRETQQMPEVGQRLRVGGMVMPGSVQRDPNSLKVTFTIYDAEGSVDVSYEGILPDL
                     FREGQGVVVQGELEKGNHILAKEVLAKHDENYTPPEVEKAMEANHRRPASVYKDPAS"
     gene            complement(23408..23617)
                     /gene="ccmD"
                     /locus_tag="NBI74_002167"
     CDS             complement(23408..23617)
                     /gene="ccmD"
                     /locus_tag="NBI74_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmD"
                     /protein_id="HCD1932500.1"
                     /translation="MTPAFASWNEFFAMGGYAFFVWLAVVMTVIPLVVLVVHSVMQHR
                     AILRGVAQQRAREARLRAAQQQEAA"
     gene            complement(23614..24351)
                     /gene="ccmC"
                     /locus_tag="NBI74_002168"
     CDS             complement(23614..24351)
                     /gene="ccmC"
                     /locus_tag="NBI74_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmC"
                     /protein_id="HCD1932501.1"
                     /translation="MWKTLHQLAIPPRLYQICGWFIPWLAIASVVVLTVGWIWGFGFA
                     PADYQQGNSYRIIYLHVPAAIWSMGIYASMAVAAFIGLVWQMKMANLAVAAMAPIGAV
                     FTFIALVTGSAWGKPMWGTWWVWDARLTSELVLLFLYVGVIALWHAFDDRRLAGRAAG
                     ILVLIGVVNLPIIHYSVEWWNTLHQGSTRMQQSIDPAMRSPLRWSIFGFLLLSATLTL
                     MRMRNLILLMEKRRPWVSELILKRGRK"
     gene            complement(24393..25055)
                     /gene="ccmB"
                     /locus_tag="NBI74_002169"
     CDS             complement(24393..25055)
                     /gene="ccmB"
                     /locus_tag="NBI74_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HCD1932502.1"
                     /translation="MMFWRIFRLELRVAFRHSAEIANPLWFFLIVITLFPLSIGPEPQ
                     LLARIAPGIIWVAALLSSLLALERLFRDDLQDGSLEQLMLLPLPLPAVVLAKVMAHWM
                     VTGLPLLILSPLVAMLLGMDVYGWQVMALTLLLGTPTLGFLGAPGVALTVGLKRGGVL
                     LSILVLPLTIPLLIFATAAMDAASMHLPVDGYLAILGALLAGTATLSPFATAAALRIS
                     IQ"
     gene            complement(25052..25669)
                     /gene="ccmA"
                     /locus_tag="NBI74_002170"
     CDS             complement(25052..25669)
                     /gene="ccmA"
                     /locus_tag="NBI74_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001300065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis heme-transporting ATPase
                     CcmA"
                     /protein_id="HCD1932503.1"
                     /translation="MLEARELLCERDERTLFSGLSFTLNAGEWVQITGSNGAGKTTLL
                     RLLTGLSRPDAGDVLWQGQPLHQVRDSYHQNLLWIGHQPGIKTRLTALENLHFYHRDG
                     DTAQCLEALAQAGLAGFEDIPVNQLSAGQQRRVALARLWLTRATLWILDEPFTAIDVN
                     GVDRLTQRMAQHTEQGGIVILTTHQPLNVAESKIRRISLTQTRAA"
     gene            complement(25688..26290)
                     /gene="napC"
                     /locus_tag="NBI74_002171"
     CDS             complement(25688..26290)
                     /gene="napC"
                     /locus_tag="NBI74_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type protein NapC"
                     /protein_id="HCD1932504.1"
                     /translation="MGNSDRKPGLIKRLWKWWRTPSRLALGTLLLIGFVGGIVFWGGF
                     NTGMEKANTEEFCISCHEMRNTVYQEYMDSVHYNNRSGVRATCPDCHVPHEFVPKMIR
                     KLKASKELYGKIFGVIDTPQKFEAHRLTMAQNEWRRMKDNNSQECRNCHNFEYMDTTA
                     QKSVAAKMHDQAVKDGQTCIDCHKGIAHKLPDMREVEPGF"
     gene            complement(26300..26749)
                     /gene="napB"
                     /locus_tag="NBI74_002172"
     CDS             complement(26300..26749)
                     /gene="napB"
                     /locus_tag="NBI74_002172"
                     /EC_number="1.9.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase cytochrome c-type subunit"
                     /protein_id="HCD1932505.1"
                     /translation="MKSHDLKKALCQWTAMLALVVSGAVWAANGVDFSQSPEVSGTQE
                     GAIRMPKEQDRMPLNYVNQPPMIPHSVEGYQVTTNTNRCLQCHGVESYRTTGAPRISP
                     THFMDSDGKVGAEVAPRRYFCLQCHVPQADTAPIVGNTFTPSKGYGK"
     gene            complement(26746..27609)
                     /gene="napH"
                     /locus_tag="NBI74_002173"
     CDS             complement(26746..27609)
                     /gene="napH"
                     /locus_tag="NBI74_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinol dehydrogenase ferredoxin subunit NapH"
                     /protein_id="HCD1932506.1"
                     /translation="MANRKRDAGREALEKKGWWRSHRWLVLRRLCQFFVLGMFLSGPW
                     FGVWILHGNYSSSLLFDTVPLTDPLMTLQSLASGHLPATVALTGAVIITVLYALAGKR
                     LFCSWVCPLNPITDLANWLRRRFDLNQSATIPRHIRYVLLVIILVGSALTGTLIWEWI
                     NPVSLMGRSLVMGFGSGALLILALFLFDLLVVEHGWCGHICPVGALYGVLGSKGVITV
                     AATDRQKCNRCMDCFHVCPEPHVLRAPVLDEQSPVQVTSRDCMTCGRCVDVCSEDVFT
                     ITTRWSSGAKS"
     gene            complement(27596..28291)
                     /gene="napG"
                     /locus_tag="NBI74_002174"
     CDS             complement(27596..28291)
                     /gene="napG"
                     /locus_tag="NBI74_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapG"
                     /protein_id="HCD1932507.1"
                     /translation="MSRSAKPQNGRRRFLRDVVRTAGGLAAVGVALGLQQQTARASGV
                     RLRPPGAINENAFASACVRCGQCVQACPYDTLKLATLASGLSAGTPYFVARDIPCEMC
                     EDIPCAKVCPSGALDREIESIDDARMGLAVLVDQENCLNFQGLRCDVCYRECPKIDEA
                     ITLELERNTRTGKHARFLPTVHSDACTGCGKCEKVCVLEQPAIKVLPLSLAKGELGHH
                     YRFGWLEGNNGKS"
     gene            complement(28298..30784)
                     /gene="napA"
                     /locus_tag="NBI74_002175"
     CDS             complement(28298..30784)
                     /gene="napA"
                     /locus_tag="NBI74_002175"
                     /EC_number="1.7.99.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="periplasmic nitrate reductase subunit alpha"
                     /protein_id="HCD1932508.1"
                     /translation="MKLSRRSFMKANAVAAAAAAAGLSVPGVARAVVGQQEAIKWDKA
                     PCRFCGTGCGVLVGTQQGRVVACQGDPDAPVNRGLNCIKGYFLPKIMYGKDRLTQPLL
                     RMKNGKYDKEGEFTPITWDQAFDVMEEKFKTALKEKGPESIGMFGSGQWTIWEGYAAS
                     KLFKAGFRSNNIDPNARHCMASAVVGFMRTFGMDEPMGCYDDIEQADAFVLWGANMAE
                     MHPILWSRITNRRLSNQNVTVAVLSTYQHRSFELADNGIIFTPQSDLVILNYIANYII
                     QNNAINQDFFSKHVNLRKGATDIGYGLRPTHPLEKAAKNPGSDASEPMSFEDYKAFVA
                     EYTLEKTAEMTGVPKDQLEQLAQLYADPNKKVISYWTMGFNQHTRGVWANNLVYNLHL
                     LTGKISQPGCGPFSLTGQPSACGTAREVGTFAHRLPADMVVTNEKHRDICEKKWNIPS
                     GTIPAKIGLHAVAQDRALKDGKLNVYWTMCTNNMQAGPNINEERMPGWRDPRNFIIVS
                     DPYPTVSALAADLILPTAMWVEKEGAYGNAERRTQFWRQQVQAPGEAKSDLWQLVQFS
                     RRFKTEEVWPEELLAKKPELRGKTLYEVLYATPEVSKFPVSELAEDQLNDESRELGFY
                     LQKGLFEEYAWFGRGHGHDLAPFDDYHKARGLRWPVVNGKETQWRYSEGNDPYVKAGE
                     GYKFYGKPDGKAVIFALPFEPAAEAPDEEYDLWLSTGRVLEHWHTGSMTRRVPELHRA
                     FPEAVLFIHPLDAKARDLRRGDKVKVVSRRGEVISIVETRGRNRPPQGLVYMPFFDAA
                     QLVNKLTLDATDPLSKETDFKKCAVKLEKV"
     gene            complement(30781..31044)
                     /gene="napD"
                     /locus_tag="NBI74_002176"
     CDS             complement(30781..31044)
                     /gene="napD"
                     /locus_tag="NBI74_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone NapD"
                     /protein_id="HCD1932509.1"
                     /translation="MHTNWQVCSLVVQAKSERISDISTQLNAFTGCEVAVSDAPSGQL
                     IVVVEAEDSETLIQTIESVRNVEGVLAVSLVYHQQEEQGEETP"
     gene            complement(31034..31528)
                     /gene="napF"
                     /locus_tag="NBI74_002177"
     CDS             complement(31034..31528)
                     /gene="napF"
                     /locus_tag="NBI74_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapF"
                     /protein_id="HCD1932510.1"
                     /translation="MKIDASRRGILTGRWRKASNGIRPPWSGDESHFLTHCTRCDACI
                     NACENNILQRGAGSYPSVNFKNNECSFCYACAQACPESLFSPRHTRAWDLQFTIGDAC
                     LAYQSVECRRCQDSCEPMAIIFRPTLSGIYQPQLNSQLCNGCGACAASCPVSAITAEY
                     LHAH"
BASE COUNT         7736 a         8661 c         7887 g         7495 t
ORIGIN      
        1 atgcggatat tttttatttc tttaatcaga aactggtcga aagtaaggcc tttttgtggc
       61 tggttgcatt gaccaacaat cgcgctttcg acggttgttc actgctggcg atgggaatgt
      121 tgatgctgag tttctggctg aaagaaaacg ccccaggcag acgacgtatc gtgattattg
      181 gtctggtcat gctattaact gcagtggtat taaaccagct gggtcaggca ttaattcctg
      241 taaaacgggc cagtccaacg ttgactttta ccaatattaa ccgcgtcagc gaactgctct
      301 ctgttcccac gaaagatgcc tcacgagata gctttcccgg cgatcacggt atgatgctgc
      361 ttattttttc ggcattcatg tggcgttatt tcggcaaagt tgctggcctt atcgccctta
      421 ttatttttgt ggtttttgcg tttcccagag taatgattgg cgcacactgg tttactgaca
      481 tcatcgtcgg ttccatgacc gtgatattga tcggtttgcc ctgggtgttg ctgacgccat
      541 taagtgatcg attaatcacc ttttttgaca aatcactacc tggaaaaaac aaacatttcc
      601 aaaacaaata actcacagta attaacatca tcaggtttat ttttatagtg aggataatcc
      661 tgatgatgcg cctcctgctt tcatccatcg agcgcaaaaa tcattctcta agtaaatgaa
      721 tggattgcat gcgtttcact caatcgtact ttaattgacc aatcccgctg attaactttc
      781 tgtatcactt tttcttataa aaaatcatgt aaaaccgctc gccaagaccg caccaatcgg
      841 gtaatctcga actcgttttg cctcggcggt agattatcct cacagcatat aattttgtgc
      901 gttagtccac agatttggcc ttaaggaatt gtttcaacat gcccaggtaa ttagtctcgt
      961 gtcgcttggc atttttttat aacgatattt gtcgttaagg acttcaaggg aaaacaaaca
     1021 acatggtcaa atctcaaccg attttgagat atatcttgcg cgggatcccc gcgattgcag
     1081 tagcggttct gctttctgca tgtagtgcaa ataacaccgc aaagaatatg catcctgaga
     1141 cacgtgcagt gggtagtgaa acatcatcac tgcaagcttc tcaggatgaa tttgaaaacc
     1201 tggttcgtaa tgtcgacgta aaatcgcgaa ttatggatca gtatgctgac tggaaaggcg
     1261 tacgctatcg tctgggcggc agcactaaaa aaggtatcga ttgttctggt ttcgtacagc
     1321 gtacattccg tgagcaattt ggcttagaac ttccgcgttc gacttatgaa cagcaggaaa
     1381 tgggtaaatc tgtttcccgc agtaatttgc gtacgggtga tttagttctg ttccgtgccg
     1441 gttcaacggg acgccatgtc ggtatttata tcggcaacaa ccagtttgtc catgcttcca
     1501 ccagcagtgg tgttattatt tccagcatga atgaaccata ctggaagaag cgttacaacg
     1561 aagcacgccg ggttctcagc cgcagctaat aaaccgtttg gatgcaatcc cttggctatc
     1621 ctgacgagtt aactgaaagc actgcttagg cagtgctttt ttgttttcat tcatcagaga
     1681 aaatgatgtt tccgcgtctt gatccaggct atagtccggt cattgttatc ttttaaatgt
     1741 tgtcgtaatt tcaggaaatt aacggaatca tgtttatacg cgctcccaat tctggacgta
     1801 agctcctgct tacctgcatt gttgcaggcg tgatgattgc gatactggtg agctgccttc
     1861 agtttttagt ggcctggcat aagcacgaag tcaaatacga cacactgatt accgacgtac
     1921 aaaagtatct cgatacctat tttgccgacc ttaaatccac tactgaccgg ctccagccgc
     1981 tgaccttaga tacctgccag caggctaacc ccgaactgac cgcccgcgcg gcgtttagca
     2041 tgaatgttcg tacgtttgtg ctggtaaaag ataaaaaaac attctgttca tctgcaactg
     2101 gtgagatgga cattccactc aatgaattga ttccggcgct cgacattaat aaaaacgtcg
     2161 atatggcgat cttacccggc acgccgatgg tgccgaacaa acccgcaatc gtcatctggt
     2221 atcgcaaccc tttgctgaaa aatagcggcg tgtttgccgc tctgaatctc aacctgacgc
     2281 cttcactctt ttatagttca cggcaggaag attacgatgg cctcgccctc attattggta
     2341 atactgcgct atctaccttt tcttcacgtt tgatgaacgt taatgaatta accgacatgc
     2401 cggtccgtga aactaaaatt gcgggcattc ctctgaccgt tcggctttat gcagatgact
     2461 ggacatggaa cgatgtgtgg tacgcatttt tactgggtgg catgagtgga actttcgttg
     2521 gacttctctg ctattacctg atgagtgtgc gtatgcgccc aggcagagaa atcatgaccg
     2581 ccatcaagcg cgaacaattt tacgtggtat atcaaccggt ggttgataca caagctttgc
     2641 gggtaacggg cctggaagta ctgctacgct ggcggcatcc tgtagcagga gaaatacccc
     2701 cggatgcctt cattaacttt gccgaagcgc aaaagatgat tgtgccactg actcagcacc
     2761 tgtttgagtt gattgcccgc gatgccgcag aattagaaaa agtactgccg gtaggcgtca
     2821 aatttggcat taacattgcg ccggcccact tgcacagcga aagctttaaa gcggatatcc
     2881 agaaactgct cacttccctg cccgcacacc atttccagat tgtgctggaa attaccgagc
     2941 gcgatatgct gaaagagcga gaagccacac aactcttcgc ctggctgcat tcggtcggcg
     3001 tagaaatcgc tattgatgac ttcggcaccg ggcacagcgc gcttatctat cttgagcgtt
     3061 ttacgctcga ttatctgaaa attgatcgtg gatttatcaa cgccatcggt acggaaacga
     3121 tcacttcacc cgtacttgac gcggtgctga cgctggcgaa acgtctcaat atgctgacag
     3181 ttgctgaagg ggtcgaaacg ccagaacagg cacgatggct aagcgaacgc ggcgttaatt
     3241 tcatgcaagg ctactggatt agtcgcccgt taccgctgga cgattttgtt cgctggctga
     3301 agaaaccgta tacgccgcag tggtaaggtg tgcttacgtc ccttattatt catagtgaaa
     3361 gcatgccgga ttgcggctaa tgatgagtaa aaggaaatcc gttgcagatg attgtgcgca
     3421 tactgctgct gtttatcgct ctgttcacct ttggtgcgca ggcgcaggct atcaaggaaa
     3481 gctatgcctt tgcagtactg ggcgaacccc ggtacgcatt taatttcaac cattttgatt
     3541 atgtgaaccc cgccgcgcca aaaggtgggc aaataacgtt gtctgccctc ggcaccttcg
     3601 ataatttcaa ccgctatgca ctgcgcggca atccgggcgc acgcaccgag cagctgtacg
     3661 acacgctatt tacgacttcc gatgacgaac caggcagtta ttacccgctg attgctgaaa
     3721 gcgcacgcta tgctgacgat tattcctggg tggaggtcgc tattaatcca cgcgcccgtt
     3781 ttcatgatgg ttcgcccatt actgcccgcg atgtagagtt tacttttcaa aaatttatga
     3841 ccgaaggcgt gccgcaattt cgtctggtct acaaaggcac caccgtcaaa gccattgcgc
     3901 cgttaaccgt gcgcattgag ttagctaaac ccggcaaaga agatatgctg agtctgtttt
     3961 cgctgccggt atttccagaa aagtactgga aagatcacaa acttagcgac ccgctcgcca
     4021 cgcctccgct tgccagtggt ccgtaccgca ttacgtcctg gaaaatgggg caaaatattg
     4081 tctattcccg cgtgaaagat tactgggcag caaacttacc ggtaaaccgt ggacgctgga
     4141 atttcgacac cattcgctac gattattacc tcgatgataa tgtcgccttt gaagcgttta
     4201 aagcaggagc ctttgatttg cgtatggaaa acgacgccaa aaactgggcc acgcgctata
     4261 ccggtaaaaa tttcgataaa aaatacatca tcaaagatga gcaaaagaac gaatcagccc
     4321 aggatacgcg ctggctggcg tttaatatcc aacgtccggt attcagcgat cgccgggtgc
     4381 gggaagcgat cactctcgcc tttgactttg aatggatgaa caaggcgttg ttttacaata
     4441 cctggagtcg cacgaacagt tattttcaga ataccgaata tgcggccaga aattaccccg
     4501 acgccgcgga actggtgctt ctggcaccaa tgaaaaaaga tctaccgcca gaagtcttca
     4561 cgcaaatcta ccagccgccg gtatccaaag gcgatggcta cgatcgtgac aacctgttaa
     4621 aagccgacaa acttctcaac gaagcaggct gggtgctgaa gggtcagcaa cgcgttaatg
     4681 ccacaacagg tcagccactc agctttgaat tattgcttcc cgcaagcagc aatagtcagt
     4741 gggtattgcc gttccagcac agcctgcaac ggctgggtat caacatggac attcgcaagg
     4801 tggataactc gcaaatcacc aaccgcatgc gcagtcgcga ctatgacatg atgccgcgcg
     4861 tatggcgggc gatgccgtgg cccagttccg atttacagat ttcctggtca tcggaatata
     4921 tcaattccac ttataatgcc cccggcgtgc aaagcccggt tatcgactcg ctgatcaacc
     4981 aaattattgc cgcgcaggga aataaagaaa aattactgcc gttgggacga gcactggatc
     5041 gcgtattaac gtggaattat tacatgctgc caatgtggta catggcggaa gaccgtctcg
     5101 cctggtggga taaattctcc caacccgctg tacgcccagt ttacagcctg ggtattgata
     5161 cctggtggta tgacgttaat aaagcgacga aactgccgtc agccagacaa cagggagagt
     5221 agatgggcgc ttatctgatc cgccgtctgt tgctggtgat cccaacgcta tgggcgatta
     5281 tcactatcaa ctttttcatc gtgcaaattg cgcctggcgg tccggtcgac caggccatcg
     5341 ccgccattga gtttggcaat gccggagtat tacccggcgc aggcggtgaa ggtgttcgcg
     5401 ccagccatgc gcaaacgggc gtcggcaata tcagcgacag taattaccgt ggcggacgcg
     5461 gattagatcc agaagtgatc gccgagatca ctcatcgcta cggtttcgat aagccgatcc
     5521 acgaacgtta cttcaaaatg ctctgggact acatccgttt tgattttggc gacagccttt
     5581 ttcgcagcgc ctcggtgctg acgctgatta aagacagtct gccggtctcc atcaccctcg
     5641 gattgtggag cacgctgatt atctatctgg tgtcgattcc gttaggcatt cgcaaagctg
     5701 tttataatgg gagcagtttt gacgtctgga gtagtgcatt tatcatcatc ggctacgcca
     5761 ttccggcctt tttgtttgcc atcctgctga ttgtcttctt cgcgggcggc agctatttcg
     5821 atctgttccc tctgcgcggc ctggtttccg ctaactttga ttcgctgccg tggtatcaga
     5881 aaatcaccga ttatctgtgg catatcacgc tgccggtgct ggcgacagtg attggcggct
     5941 ttgcggctct gaccatgctg acaaaaaact cattccttga tgaagtgcgt aagcaatacg
     6001 tggtgacggc acgtgcgaaa ggggtaagtg aaaaaaatat tctctggaaa catgtgttcc
     6061 gcaacgccat gctgctggtg attgccggtt ttccggcgac gtttatcagc atgtttttta
     6121 ccggctcgct gctgattgag gtgatgtttt cactcaatgg tcttggctta ctgggctacg
     6181 aagcgaccgt ctcgcgcgat tatcctgtaa tgtttggtac cttgtatatt ttcaccctga
     6241 ttggcctgct gctgaatatt gtcagtgata tcagctatac gctggtcgat ccgcgtatag
     6301 actttgaggg acgttaatgt cgcgactcag ccccgtcaat caggcccgtt gggcgcgttt
     6361 tcgccataac cgtcgcggct actggtcgtt atggattttc ctcctcttgt ttggtttgag
     6421 tttgtgttct gaacttatcg ccaacgataa accgttgctg gtgcgttatg acggcagttg
     6481 gtatttcccg ttgttgaaaa actacagcga aagcgatttt ggcggcccgc tggcaagtca
     6541 ggctgattat caggacccgt ggctgaaaca acgactggaa aataacggct gggtactgtg
     6601 ggcaccgatt cgctttggtg ctaccagtat caactttgct accgataagc ccttcccttc
     6661 tccaccctcc cggcaaaact ggctgggaac ggatgccaat ggcggcgatg tgctggcgcg
     6721 cattctctat ggcacgcgga tctcagttct gtttggcctg atgctgaccc tctgctccag
     6781 cgtgatgggt gtgctggcgg gggcgctaca aggctattac ggtggtaaag tcgatctctg
     6841 ggggcaacgt tttattgaag tatggtcggg gatgccgacg ctgtttttga ttattttact
     6901 ttccagcgtc gtacagccta acttctggtg gctgctggca attactgtct tgtttggctg
     6961 gatgagtctg gtcggcgtgg tgcgggcgga gtttttacgt acccgtaatt tcgactacat
     7021 ccgcgcggcg caggcgcttg gcgtcagcga tcgcagtatc atcctgcgtc atatgttgcc
     7081 aaatgccatg gtcgctaccc tcactttttt accgtttatt ttatgtagtt cgataaccac
     7141 cctgacctca ctcgatttcc tcggcttcgg tctgccgctc ggttcaccgt cactcggcga
     7201 actcctgtta caagggaaaa ataaccttca ggccccgtgg cttgggatca ccgccttctt
     7261 atcggtggcg atattgttgt ctttgctgat ctttattggt gaagccgtcc gcgacgcatt
     7321 tgatcctaat aaggcggtgt agcatgacgc aaactctgtt agcgattgaa aatttgtcgg
     7381 tgggttttcg ccatcagcaa actgttcgta ccgtagtgaa tgatgtgtca ctacacattg
     7441 aggctggcga aacgctggcg ctggtaggtg agtctggttc aggtaagagc gttaccgcgc
     7501 tgtcaatttt acgcctgctc ccttgcccgc cggttgaata tctctccggc gatattcgtt
     7561 ttcatggcga atcgctgctt catgccagcg atcaaacgtt gcgcggtgta cgcggtaata
     7621 agatcgccat gatttttcag gagccgatgg tgtcgttaaa tccattacat accctggaaa
     7681 aacagcttta tgaagtgctt tcactccacc gcgggatgcg tcgggaagcg gctcgtggcg
     7741 aaattcttaa ctgccttgat cgcgtcggta tccgccaggc ggcaaaacgg ctgacagatt
     7801 atccgcatca gctctccggc ggcgaacgcc agcgggtgat gattgcgatg gcgctgttaa
     7861 cgcgaccgga attattaatt gccgatgaac caaccaccgc gctggacgtc tctgtccagg
     7921 cgcagatttt acagctgttg cgcgaactgc aaggcgagct gaatatgggt atgctgttta
     7981 ttactcataa cctcagcatt gttagaaaac tggcccaccg cgtggcggta atgcaaaacg
     8041 gtcgctgtgt cgagaaaaat aacgccgcta cgctatttgc ctcacccact catccttaca
     8101 cacaaaagct actcaacagt gaaccatctg gcgacccggt gccattgcca gaacctgcct
     8161 caacgttgct ggatgttgaa caacttcagg ttgccttccc cattcgcaaa gggattttga
     8221 agcgcattgt ggatcataat gtggtggtga aaaacatcag ttttacgcta cgggcgggtg
     8281 aaacactggg tttagtgggc gagtccggtt ccgggaaaag cacgacggga ctggcgctgc
     8341 tgcgactgat taactctcat ggcagcatcg tctttgacgg tcagccactg caaaatttaa
     8401 atcgccgcca gctgttacct attcgtcatc gcattcaggt ggtatttcag gatccaaact
     8461 cctcactcaa cccacgactc aacgttttgc aaattattga ggaaggctta cgggttcacc
     8521 agccgacgct ttctgccgca caacgcgaac aacaagtgat agccgtgatg catgaagtgg
     8581 gattagatcc tgaaacacgc caccgttatc cggcggagtt ctctggtggt cagcggcaac
     8641 gtattgcgat tgccagggcg ttaattctta agccctcgct gatcattctt gatgaaccaa
     8701 catcatcact cgacaaaacg gtgcaggcgc aaatattgac gctattgaaa tcattgcaac
     8761 aaaagcatca actggcctat ttgtttatca gccacgattt gcacgttgtc cgcgcgttat
     8821 gtcatcaggt tatcgtactg cgacaagggg aagtagtgga acaaggaccg tgcgcgcgcg
     8881 tgtttgccgc accgcagcag gagtatacgc gtcagctact ggcgttgagc tgacgcttaa
     8941 aaaggattgt agtttgaaaa gggttcggca attgccacac caaaattttt caatcggcat
     9001 gttgcagcaa attcgtcctg gcgatttaca aacaggcacg gctcaccttc acactccagc
     9061 accgaacatg gaacttcgat gtcgcttaac gcctggctta gtttgtacat caccgaccat
     9121 gcattatcac catccgggct aagcaattta agcgccacca ggctgttgtc accgcacggt
     9181 ccattttgcg gaatcggttc aaccttcgaa cctgcgaaac cagcagacca gcgatagttc
     9241 tgcggcagtc gatggacgat tgagagttgt aatgaagtca cttattttcc ccggaagtac
     9301 attttacttc acaatttatt tacatcaatt ttaacacatc atcaacaatc cgtctttata
     9361 cagatcgtaa agattgatgc ctcgcctgcg tggcctatat ggctctgatt taaataatat
     9421 ctgcgggcgt ggtcacgttt gcggggctat atgtacccgt ttctgcgatc taactcaacc
     9481 tttttaacta caatgatgtg actttttaca taaattgatt ttacataaaa taaacatata
     9541 tcgggggaat gatagatttg tggttgacat gcatcaacaa aggaagcctt ttagcttcct
     9601 cgttgtgcaa tagatcaccg ttttttcggc cgactggcgt acagacagaa gagaatggag
     9661 ctggttgcac agaatgcaat tgaccaaatc atcggccatg cagagttaaa ggtcgcaaga
     9721 gaaagtaatg cgccaacaat tgcccctatg ccgaaacgga aggttcctgc cagcgaagat
     9781 gccgttcccg ccatatgggg aaactcatca agaatgaccg ccatcgcatt ggatgacacc
     9841 atcgacacgc agcccacaaa cgccgcaacg ccaaccacca gcgaccagaa tcccagcccc
     9901 agcagtgcac tgatgaccat ccacgctgcc ataataaact gtatccacaa ccccgagcgg
     9961 aacatattta acgcgccaat acggcggacg aagcggctgt taaagatggt catcacgaac
    10021 agaaaaagaa tgtttagcgc aaagtaataa ccaaagtttt ccggcgcgac gtggttgatt
    10081 tcaatgtaga caaacggtcc ggcgcttaag aatgagaaca tcccggcaaa gctgaaaccg
    10141 ctggccagca tataactcag gacacgctta tgacggaaca gcgccgcaaa gttaccaata
    10201 gtggtacgaa tgtgaaaagg ctggcgacgc tccggtggta aggtttcttt gatcagaaag
    10261 aagatcattg ccgaagccag aatcgccgcc acggcgagga tccagaagat gtaatgccag
    10321 ctcagccaca ccagcaccca gccgccaact atcggtgcca tcagcggtgc aatggtcgtc
    10381 accagcatga caaacgacat catccgtgag aactcttctt tcgggtagat atcacgcatc
    10441 aaagcgttga taaccacgct ggccgcagcc gcagccagcc cgtggaagaa acgcatcaca
    10501 atcagctgat cgatggtttg tgccaacgca cacgccaccg cggcggcggc aaacaccagc
    10561 gtaccgccaa gaaccaccgg cttacgcccg aagctgtctg ccatcggccc gtagattaac
    10621 tgccccaacg caaagcccag aatataagta ctgagggtca tctgcgtact gcccgccggt
    10681 acaccaaact gcgctgaaat taccggtagc gcaggcagat acatatcaat cgacagcggc
    10741 atcaacatgg ccagcaggcc aaggataaaa acaatagcaa acgacgaatg ctgtcgggtg
    10801 gtcacaacgg gctcctgaaa gtcattgaaa agttagacga cgctggcaat ttcttcttca
    10861 gttaacggac gatattcacc gggggctaaa tcagcatcca gcgtaatacc gccaatacgt
    10921 tcacgatgca gctcaaccac atggttaccc acggcggcga acatacgttt cacctgatga
    10981 taacgccctt cgctgatggt caaacgaacc tgcgttgggg taatcacttc cagcaccgcc
    11041 ggcttagtga gatctttttc gttatgcagc tgcacgcctt tagcaaattg ctctgccgta
    11101 tcgtcagcta cgggtgattc cagcgtcacc agataggtct tctcgcaatg atggcgcggg
    11161 gaagtaatgc ggtgcgacca ctgaccatca tctgtcatta gtaccagacc ggtggtatcg
    11221 atatccagac gccctgcagc gtgcagtttc cacgctaccg gttcatcaag aaaatagagc
    11281 accgttgggt gatccgggtc gtccgtggag caaacatagc cctgaggctt attgagcatg
    11341 aagtaacgtg gaccgtgttg ctgcgccagc gggttgccat cgtaagcgac atcatgttca
    11401 gggagcagtt tgaacgctgc attacggacg atttcgccat cgacggtgac acgattgccg
    11461 cggatttcac gcccggcaat agcacggcta acgccgagtt gctgtgcgat aaatttatca
    11521 agtcgcatgt gtgtgatttt gcctgtaaaa aacggaggtc gggtaatgac ccgaaaatct
    11581 gaactgttgt ctgcccagta tagcggtcta ataacttccc tcaagggaaa aagatccgtg
    11641 gcatactatt agcagaataa tctacctacg cgagaccatg atttttacac tccgcccata
    11701 tcagcaagaa gccgtggatg ccacgctcaa ccattttcgt cgtcataaaa cccctgccgt
    11761 aatcgtgctg cccaccggtg caggtaaaag cctggtgata gcggaactgg cgcggctggc
    11821 gcgtggtcgc gtgctggtgc tggcacacgt taaagaactg gtggcacaaa accatgcaaa
    11881 atatcaggcg ctggggctgg aagccgatat ttttgccgcc gggctaaagc gcaaagagag
    11941 ccacggtaaa gtggtatttg gcagcgtgca gtcggtcgcc cgtaatcttg atgcctttca
    12001 gggtgaattt tcgctgttga ttgtcgatga atgccaccgt attggtgacg atgaagagag
    12061 ccaatatcag caaatcctca ctcacctgac caaagtgaat ccccacttac gcctgctggg
    12121 gctgactgcc acgccttttc gactgggcaa aggctggatc taccagttcc attatcacgg
    12181 catggtacgc ggcgatgaga aagccctttt ccgtgactgc atttatgagc tgccgctgcg
    12241 ttatatgatt aaacacggct atctgacgcc gccagaacgg ctggatatgc cagtagtgca
    12301 atacgatttc agccgcttgc aggcacagag taacggactg ttcagcgaag ccgatctcaa
    12361 ccgcgagctg aaaaaacagc aacgcattac gccacacatc atcagccaaa tcatggagtt
    12421 tgctgcaacg cgcaaagggg tgatgatttt cgccgcgacg gtcgaacacg caaaagagat
    12481 agtgggatta ctgcctgccg aagatgcagc actgattact ggcgacacac ccggctctga
    12541 gcgcgatgtg ttaattgatg attttaaagc ccagcgtttt cgctatctgg tcaacgtcgc
    12601 ggtactgacc accggatttg acgccccgca cgtcgatctt atcgccattc tgcgccctac
    12661 cgaatcggtg agtctttacc aacaaattgt cgggcgcggt ctgcgcctgg cccccggcaa
    12721 gactgattgc ttaattcttg attatgcggg taaccctcac gatctctacg cgccggaagt
    12781 tggtacaccg aaaggcaaaa gtgacaacgt tccggtacag gttttctgcc ctgcctgcgg
    12841 ttttgccaac accttttggg ggaaaacgac cgccgacggg acattgattg aacactttgg
    12901 tcgtcgctgt cagggatggt ttgaagatga cgacggacat cgcgaacagt gtgacttccg
    12961 tttccgtttt aaaaattgcc cgcaatgtaa cgctgaaaat gatattgccg cccgccgttg
    13021 ccgcgagtgt gacaccattc tggttgaccc ggatgatatg ttaaaagcgg cgctacgact
    13081 gaaagacgcg ctggtattac gctgtagcgg catgtctttg cagcatgggc acgacgaaaa
    13141 aggcgaatgg ttgaaaatca cctattacga tgaagacggc gcggatgtga gtgagcgttt
    13201 ccgtctgcaa acgcccgccc agcgaactgc cttcgagcag ctttttatcc gcccgcatac
    13261 gcgcacaccg ggcatcccgc tgcgctggat caccgccgcc gatgtcctcg cccagcaagc
    13321 cttattgcga cacccggatt ttgtcgtcgc ccgcatgaaa ggccagtact ggcaagtgcg
    13381 tgaaaaagtg ttcgattacg aaggtcgttt tcgtcgggcg cacgaattac gcggttaatg
    13441 gtagttttga ttgatgtata aggcgattga gtatagaatc tcgcccgctt ttgcatacgc
    13501 aaagcagatc acttacctgt tgctgggtcg cctgtagcag gattaattta agagagaaag
    13561 aaatgtttac tatcaacgca gaagtacgta aagagcaggg taagggtgcg agccgccgcc
    13621 tgcgtgccgc taacaagttc ccggcaatca tctacggtgg caaagaagcg ccgctggctg
    13681 tcgaactgga tcacgacaaa gtcatgaaca tgcaagttaa agctgaattc tacagcgaag
    13741 ttctgaccat cgttgttgac ggtaaagaaa tcaaagttaa agctcaggac gtacagcgtc
    13801 acccgtacaa accgaagctg ctgcacatcg acttcgttcg cgcttaattg ctgaataagt
    13861 tgtaaaaaac cccgctccgg cggggttttt tgtctctgca aattatgcct gattacggta
    13921 ttgctatttt ttgcaggcca gataaggcgt tcacgccgca tctggcattc agaacacaaa
    13981 accgattaat tgccgccaga cgtccggcgc tgcaattgat cgcgcaagtt cggcggcgtg
    14041 cctttaatgg tcagggtatc ggttgctgga tcccagaaga tgcgttcacc taacagcatc
    14101 gcatcaaagt taatagtcaa gccaccgccg ctaccggcaa acttcgtcaa ctgacgcagt
    14161 gtgctacggt ctgccgggaa gctctcttcc agttcgtagc ctttttcagc cgcaaactcc
    14221 gtgaagctca cttcgctgac gcctgccagc tctttcgaca gcgattccag ttcaatctct
    14281 tcacctgcct gtagctgctc attacagtag ctataaacct gctggcgcac gttctgtcgc
    14341 tctgctttat ccagttgtgc ttcggcagtg aaatcatcga ccgcttgcaa cagcccacgg
    14401 ttttgcgctt tcgcgtttaa cccttcgctg gcaccgagga aatccataaa gaaatccgcc
    14461 actttgcgcc ctactcgccc tttcaggaaa gtgagatagc gggtcgactc tggattggtt
    14521 tcccactcgg ttaaatcgat acgcgcaaca atatccgcat gattgatatc aagataatgg
    14581 gttgggttga tgtcgagatt ttcgttaaca cgcatactgc tcaggttgct cagaactgcc
    14641 accagcagat actccaccgc cagatagcga tagtggcaaa acagcacgaa tccaccgtca
    14701 gcaaaaggat atttcgctaa ttcgtcacgc aggcgaccgg ttgccgcgcg gctaaatgcc
    14761 agaaagtcct cttcgccctg acgctgtaag cgcagcgtct gcgccagttc actctcttcg
    14821 ctaaacaagc cgtaagcttt atttttggcg ctatagaccc gatgcagttc tgccaccatc
    14881 tcaacgacgg tttccgtcgg ttccagcaat gaatcgcgca acaccagctc aaggttctgc
    14941 tcatcacgct tgataagctg gtgcagagca atctggttga tatccagact catgataaac
    15001 tctcctttaa gaccgggcgg tattcaacca ccgccggtta agcgacgcaa taaaagatgg
    15061 cggtcatttt acagaaggcg gcgtaacgag aatgatttaa cgcataaaaa agcagaaaaa
    15121 aaaccgttgc tacggtaata tgttgccctt tcatcaacta actgatttcg atttatgcca
    15181 caaatttccc gctacagtga tgaacaggtt gaacaattgc tcgcagagct gctcaacata
    15241 ctggaaaaac ataaggctcc gaccgacctt tccctgatgg tgttaggcaa catggtcact
    15301 aaccttatca acaccagcat tgccccggcc caacgccagg caattgccaa ctcttttgcc
    15361 cgcgccttac agtcctctat caacgaagac aaagcgcact aagggaaaca gataacaggt
    15421 tatggtaact catcgtcagc gctaccgtga aaaagtctcc cagatggtca gttgggggca
    15481 ctggtttgca ctgttcaata ttctgctttc gctcgtcatt ggcagccgtt acctgtttat
    15541 cgccgactgg ccgacaacgc ttgctggtcg catttattcc tacgtaagca ttatcggtca
    15601 tttcagcttc ctggtgttcg ccacctactt gctgatcctc ttcccgctaa cctttatcgt
    15661 cggctcccag aggctgatga ggtttttgtc cgtcattctg gcaacggcgg gaatgacgct
    15721 attactgatc gacagcgaag tctttactcg tttccatctc catcttaatc ccatcgtctg
    15781 gcagctggtt atcaacccag acgaaaatga gatggcgcgc gactggcagc tgatgttcat
    15841 cagcgtgccg gttattttat tgcttgaact ggtgtttgcg acgtggagct ggcaaaagct
    15901 gcgcagcctg acgcgtcgtc ggcgcttcgc gcgcccgctg gccgcattct tatttatcgc
    15961 ctttatcgcc tcgcatgtgg tttatatctg ggccgatgcc aacttctatc gcccgatcac
    16021 catgcagcgc gctaacctgc cgctttcgta cccgatgacg gcacgacgtt ttcttgagaa
    16081 gcatggcctg cttgatgcgc aagagtatca acgccgtctt attgagcaag gcaatccaga
    16141 cgccgtttcc gttcagtatc cgttaagcga actgcgctat cgcgatatgg gcaccgggca
    16201 gaatgtgctg ttgattactg tcgatggcct gaactactca cgcttcgaga agcagatgcc
    16261 tgcgctggca ggttttgctg agcaaaatat ttcgttcacg cgtcatatga gctccggcaa
    16321 cactacagac aacggcatct ttggcctgtt ttatggcatc tcgccaagct atatggacgg
    16381 cattttgtcg acccgtacgc ctgcggcgtt aattactgcg cttaatcagc aaggctatca
    16441 gctggggtta ttctcgtcag atggctttac cagcccgctg tatcgccagg cattgttgtc
    16501 agatttctcg atgccgagtg tacgcaccca atccgacgaa cagaccgcca cgcagtggat
    16561 taactggctg ggccgctacg cacaagaaga taaccgctgg ttctcgtggg tctctttcaa
    16621 tggcacaaac attgacgaca gcaatcagca ggcatttgca cggaaatata gccgggcagc
    16681 aggcaatgtc gatgaccaga tcaaccgcgt gctcaatgca ctgcgtgatt ctggcaaact
    16741 ggacaatacg gtggtgatta tcactgccgg gcggggtatt ccgctgagcg aagaggaaga
    16801 aacctttgac tggtcccacg gtcatctgca ggtgccatta gtgattcact ggccaggcac
    16861 gccggcgcag cgtattaatg cgctgactga tcataccgat ctgatgacga cgctgatgca
    16921 acgcctgcta catgtcagca cacctgccag cgaatattcg caaggtcagg atttgttcaa
    16981 ccctcaacgc cgccattact gggttaccgc cgcggataac gatacgctgg caattaccac
    17041 cccgaaaaag acgctggtgc tgaacaataa cggtaaatac cgcacttaca acttacgtgg
    17101 tgaaagagtg aaagatgaaa aaccacagtt aagtttgtta ttgcaagtac tgacagacga
    17161 gaagcgtttt atcgctaact gattaattat aaatcagtta gcaaaatatc ttacttgcaa
    17221 tcggtgtgga aaacggtagt attagcagcc acgagtcggc acgtagcgca gcctggtagc
    17281 gcaccgtcat ggggtgtcgg gggtcggagg ttcaaatcct ctcgtgccga ccaaaaatcc
    17341 caagaaaacc aacctattgc ggttggtttt tttatatctg catttaattc gataaacaga
    17401 cagcgacaca tcacggcctg tttatttctg ttatcagaac gtccagacta cacccgcctg
    17461 agttgtgctg ctttcttcac gggagaaacg gctgtcgata ccaagcgtta gccccgtcaa
    17521 tgtttgctca aagccagcgc ccgcatcagt ggtcacgttg ttgcggctgt taattgccga
    17581 actccacatc gccgtatcgt aattaacgcc ttttacgcta ccaagacgcc cacgcacggt
    17641 gtccagttct gcatcaaata ccagcggttg tgctgccgcc atattcagca catccgtggt
    17701 tgaaggggtc atttgtgcgc gattctctgc gagactccag ctatggttgc cattatccag
    17761 caaggtatat tcatacgtac tgatatcaac aacgcttccg gcattgccca acgtaaatgc
    17821 agcatcaccg ccgcccgttg ttaccagtgt aaggctatct cctgctgccg ggctggcacc
    17881 ggtgtccgtc acgaatattt tgaaatcacc tgttgcttta acctccagta gtcctccctt
    17941 ttctataacg gcaaagacgg caatcccatc tttttcaaca attaagcgcc cgcctttaat
    18001 cgtgggatca ttcgccgcgc cataaacctg tagcactcca ttattgatcg tagtctgatt
    18061 taccgcgcca cccatttgaa caatttgata accatcattt aatactgcgt attctgcggt
    18121 gccatttatt tcaatgtact gatatccccc gttaatttca gtattactac ttacaccaaa
    18181 ttcttatata tgctgttctc cgccaggatt gattttcgta ttattggcgg ccccataaac
    18241 cagctggata tctttttcca ggacaacacc atcaactgtc tcaccatact caacagttga
    18301 tgctaatgct atgggtgaat ataacgctgt agccaccaga acagatattg cggaacgaca
    18361 aagagaaaca gaaccagatt ggtgcattaa gctttcatcc tatgaaatta attgctgtta
    18421 aaagcattgg ggacagaaaa tacccatagc tccatacccg gagtcagttt taaaaaactg
    18481 tttaaaaaaa tgcacaagta ttgtgattga ttttttagtt gtttttcctg atgagaagct
    18541 gatgcaaaat tccgtcttta taatgaagat gatgccaaag cgaacgataa agttgtagtt
    18601 ttcactacat gtccatacat aaaatggggt aacattcacg cgcctggtag cgttaccaac
    18661 gctacgctca aacataatga ttctaataaa acctcaggag actactatgc ctgaagcaac
    18721 gccttttcag gtgatgattg tggatgatca tccacttatg cgacgcggtg ttcgtcagtt
    18781 actggagctt gattctggct ttgaagtggt cgccgaagcg ggcgacggcg caagcgcgat
    18841 cgatctggcg aatagactgg atatcgacgt gatcttgctg gatctcaata tgaaaggcat
    18901 gagtggcttg gataccctca atgccttgcg cagggatggc gttaccgcgc aaattattat
    18961 cctgaccgta tcagatgcct ccagcgatgt ctttgcactg atagacgcag gcgcagacgg
    19021 ttatctgttg aaagacagcg acccggaagt gttactggaa gcaattcgtg ccggagcgaa
    19081 aggcagcaaa gtctttagcg aacgcgtcaa tcagtactta cgtgaacgtg aaatgtttgg
    19141 cgcggaagaa gaccccttca gcgtgctgac agaacgcgag ctggatgttc tgcacgagct
    19201 ggcacagggg ctgtcaaata aacagattgc ctcggtgttg aatatttccg agcagacagt
    19261 aaaagtccat attcgcaatc tgctgcgtaa actcaacgtc cgctcacgcg tggcggccac
    19321 cattctgttc ctgcaacaac gcggggcaca ataaaaatag cccgatggca tcaccatcgg
    19381 gccacttttt atttactctc ctgcggcgac aaatgttgca tcgcctgcgc gatactacgt
    19441 tcgatcaccg cacggcgagt atcgttggca ggtaagagtt tcaacatcat ctcccacgcg
    19501 gcaacggctt cgccaaatcg ttgctgctca aaggcattaa atgcatacat gcttaacaca
    19561 cggatattgc tatggtccgt tctcaccaac tggcgcagca gttcgccgcc gaggcggttg
    19621 tcgttgggat cagatgaacg tgtcaacgct tcagcgtatc ccagtgcagc atcgctgttt
    19681 ttcggatcga ggcgatacgc agtggcgtat gcatcggtgg cgatactggc gttacccagc
    19741 gccatgccaa cgcggcccaa cataatccag ccttctatat ctcccggatt tttttgcagt
    19801 tgagtacgca tccccagcgc aaggcgcgac atctcttctt cgttgagcgg atcggctttc
    19861 ggatccagcg ccctgtccag taacgccgga gcctgtgccg tggcctgctg ccagattttc
    19921 acctgctgat aattgccagt ctggtagtag ctgacgccag ccacaattaa cgccaccaca
    19981 atacccggca gataaacaac acatccggca cgcttacctt ccggcacgct ttgttctgga
    20041 aacgcttccg gcaccacgcg tacccgacgc cgcgaacggg cgtatatgac ccaaccgcca
    20101 atgccaatag ccactactgg cagcacccac agcagcacgg tcagcggcgt taacggcgga
    20161 tcgtaagtga cgaagttgcc gtaacgcgcc accatataat cgacaatctc tttcttactt
    20221 ttgccttcct gcatcagttc atacactttc tggcgcaggt cggtggcaat catcgagttg
    20281 gaatcggcaa tgctgttgtt ctggcatttc gggcagcgca gttcttcagt gagctgacgg
    20341 aactgttgtt cctgtgcttc atctttaaac tgcaacacgt cgatggtcgc cagcgctgag
    20401 ccggagatca tcagcatcag cacgcccaat aaaaacctca ttgtgcggcc tccttactgt
    20461 atttctccca cagtggcttg atctcttctt cccagacgcg aggattgaga tcgcccgcat
    20521 ggcgatagcg aatgatgccg ttgccgtcaa taaggaacgt ttctggcgcg ccatagacac
    20581 cgagatccag ccctaacatg ccgtcgccat caaacaggct tagcgcgtaa ggattgccca
    20641 gctctttcag ccagctgatt gccttctggc gatcgtcttt atagttcatg ccgaccacgc
    20701 ggatgccctg cgcagaaagc tgattcagat attgatgttc cgcacggcag gtcggacacc
    20761 aggtcgccca gacgttaagc agcactggtt tgccctgagt cagcacatcc gcctgataaa
    20821 actgccccgg attgtccagt gattcgaggc gaaacttcgg cactggcttg ccaatgagcg
    20881 ccgattccag attggtcgga tcatcccctt cggcattacg cgccagctgc cacagcagcg
    20941 ccgcggcaat cgccaggaag ataatcaacg gaattaacaa taccttgcgc ttcatgcagc
    21001 ctccggcgca gttttctgcg ggttcacgcg cttacgatag cgaggatcaa acagacacag
    21061 cagtccgccc aacgccatca tcagcccgcc cgcccaaatc cagcgaacaa atggtttgta
    21121 gtaaagacgc acggcccacg cgccgttttc cagctcttca ccgagcgccg cgtacaggtc
    21181 acgcgtgatg ccaccgtcaa tcgccgcttc ggtcatcatc gacccggcag tgttgtaata
    21241 acgtttttct gcatacagca ctgtttccgg cttgccatct cgcgttacgc cgatagtcgc
    21301 cacgccaccg cgccagttcg gaccagtcac ttctttgaca tcacggaagg tgaagcgata
    21361 ttcatgaata tcgacgctat cgccggactt catgcgcaca tcacgctcaa cgctatagtt
    21421 ctgactaaaa gcaatgccaa caattgtcac tgccagcccc aggtgagctg ccaccatccc
    21481 ccaataactg aaggtggttt tcgtgccgcg tgaaatacgc agcgcagctt ccgcaattgc
    21541 cagcaccgca atccagcagg ccattgccag gccgagcacc gtcatcgcca caactttgct
    21601 ttcgaacagc cacggcaaca gcagcgacag caccagcgta gagatgaagg cgataatcaa
    21661 caaattgcgg attttacgtg ggcgatcgcg tccccagcgc accagagggc cgacaccaag
    21721 cagtagcgca aacggcacca tcagccaggt aaacatggtg ttgaagaacg gttcgccaat
    21781 cgaaatactg cccagtccca gttgcttgtg caccagcggc agcaacgtcc ccagcaacac
    21841 caccagcatc gcggcgacca gcaaaacgtt gttcgctaac agcaaagatt cccgcgacca
    21901 cagcgcattg tttacgcgtg agcgaacttt gtgtccacgc gcggcaaaca gcagcagcga
    21961 accgccaatc accagcacca taaaggcgag gataaacata ccgcgcgccg gatcagacgc
    22021 aaacgcgtgt accgatacca gcacgccgga acgcaccagg aaagtcccca gcagacacaa
    22081 cgagaaggca ctgattgcca gcagtaatgt ccacgctttg aagctggcgc gttgttcagt
    22141 gaccgccagt gagtgcatca gcgcagtccc caccagccac ggcataaacg aggcgttttc
    22201 taccggatcc cagaaccacc agccgcccca gccgagttcg taataggccc atgcggaacc
    22261 gagcacaatg ccgagcgtca ggaagatcca cgcagccagc gtccacggac gagtaaaacg
    22321 cgcataagtg ctgtccagac gcctactcaa caaagatgca atggcaaaag caaacgccac
    22381 cgagaaaccg acgtacccca tatagagcag aggcgggtgg aaaatcagcc ccggatcctg
    22441 caacagtggg ttaagatcgc gcccttcaat cgggaaattc ggcagcgtgc gtgagaacgg
    22501 gttagaggta aagagaatga acagcaaaaa gccgacactg accatcccca ttatcgccag
    22561 tacgcgggca acaatatcca gcggaatacg ctgactaaaa atcgctaccg caaaggtcca
    22621 gccgctcatc agtagcaccc acagcaggag cgagccttca tgcgcgcccc aggtagccgc
    22681 cacgcgatac cacaccggaa gctgggtatt ggagttgctg gcaacatagg tgacggtaaa
    22741 gtcgttgacc acgaaagcat tgaccagcac cagaaatgcg ccagccacag acataaacag
    22801 cagccaggca aacaagcggg aagacgccat catgcgcgcg tccccgcgcg ccacgcccca
    22861 tagcggatac acagacagca gcagcgcaat tcctagcgcc agacacagca gcccgttacc
    22921 aatttctggc atcatgatgc tgggtcctta taaacactcg ccgggcgacg gtggttagct
    22981 tccatcgctt tctcaacttc tggcggcgtg tagttttcat cgtgtttcgc cagcacttct
    23041 ttcgcgagga tatgattgcc tttttccagt tcgccctgca ccacaacgcc ctgcccttca
    23101 cggaacagat ccggcaaaat gccttcgtaa gagacatcta ctgaaccttc agcgtcgtaa
    23161 atggtgaaag ttacttttag cgaattggga tcgcgctgca cactccccgg catcaccatc
    23221 ccgccaacgc gcaggcgctg accgacttcc ggcatctgct gagtttcacg cttgccgtag
    23281 agaatctccc ccggcgtata aaagagatcg atattcgagc gcagcgcata tagcaccaga
    23341 ccgatagtca gcgccagccc cgccagcacg gcacaggcaa tccacaagcg gtttttacgg
    23401 cgaatattca tgcagcctcc tgctgttgcg cagcacgtaa acgcgcctca cgcgcccgct
    23461 gttgcgccac gccacgcaga attgcgcgat gttgcatcac cgagtgcacg accaaaacca
    23521 ccagcggaat aacggtcatc accaccgcca gccagacaaa aaaggcgtaa ccgcccattg
    23581 cgaaaaattc attccaggaa gcaaatgcag gggtcattta cggcctcttt tcagtatcag
    23641 ttcactcacc cacggacggc gtttttccat cagcaaaatc aaattacgca tccgcatcag
    23701 cgtcagcgtg gcagacagga gcaggaagcc aaaaatcgac cagcgcagcg gcgaacgcat
    23761 cgccggatcg atactttgct gcatccgcgt tgatccctga tgcagggtgt tccaccactc
    23821 cacggagtaa tgaataatcg gcagattcac cacgccaatc agcaccagga tacctgccgc
    23881 acggcccgcc agacgacggt cgtcgaaggc gtgccacagg gcaatgacac caacatacag
    23941 aaacagcagc accagttcag aagtcagacg tgcatcccat acccaccagg tgccccacat
    24001 cggttttccc catgcagagc cggtaaccag ggcaataaag gtaaacacgg caccaatggg
    24061 ggccatcgcc gccaccgcca ggttggccat tttcatctgc cagacaaggc caataaacgc
    24121 cgccaccgcc attgatgcat aaatgcccat cgaccagatc gccgcaggca catgcaggta
    24181 gataatgcgg tagctatttc cctgctgata atcagccgga gcaaagccga atccccagat
    24241 ccagccgacg gtaagcacga ccacactggc aattgccagc cacggtataa accagccaca
    24301 gatttgatac agccgtggtg ggatcgccag ttgatgcagt gttttccaca tagtttcgat
    24361 accagactcg aacaaaaatc agtaatccag cgttattgaa tgctgattcg taacgctgcc
    24421 gccgtcgcaa aaggacttaa tgtcgcggtg cctgccagca acgcgcctaa aattgccaga
    24481 tatccgtcaa cgggcaaatg catagaagcc gcatccatcg ctgcggtggc aaagatgagt
    24541 aatgggatag tcagcggtaa caccagtatg ctgagcagca caccaccgcg cttaagtccc
    24601 actgtcagcg ccacgcccgg cgcaccaaga aagccaagcg taggggttcc cagcagtagc
    24661 gttaacgcca tcacttgcca gccataaaca tccattccca gtagcattgc taccaggggc
    24721 gaaaggatga gtaacggcag accggttacc atccagtgcg ccatcacctt cgccagcaca
    24781 acggcgggca agggtaacgg taacaacatc aattgttcaa gactgccgtc ctgcaaatcg
    24841 tcacggaaca gtcgctcgag tgccagcaag gatgaaagca gcgcggcaac ccagataata
    24901 cccggcgcaa tacgcgccag cagttgcggc tccggaccga tactgagcgg aaaaagggta
    24961 atgacaatca ggaagaacca cagcgggttg gcgatttcgg cgctgtggcg aaacgctacg
    25021 cgcagctcaa gacggaaaat gcgccagaac atcatgcggc cctcgtttgc gtcagtgaaa
    25081 tgcggcgaat tttactttca gcaacgttga gcggctggtg ggtagtcaga atcacaatcc
    25141 ccccctgctc cgtatgctgc gccatacgct gggtcagacg atcgacgccg ttaacgtcaa
    25201 tcgcggtaaa aggctcgtcg agtatccata acgtggcacg ggtcagccac agacgcgcta
    25261 aagcgacgcg gcgttgttgc ccggcagaaa gttgatttac cggaatatct tcgaatccgg
    25321 caagcccggc ctgcgccagg gcttccagac attgtgcggt atcgccatcg cgatgataaa
    25381 agtgcagatt ttctaacgcc gtcagccggg ttttgatccc cggctgatgg cctatccata
    25441 acaggttttg atggtagctg tcgcgtacct gatgcaaggg ctgcccttgc cagagaacgt
    25501 cacccgcatc agggcgagac aaccccgtca gcaaacggag aagcgttgtc ttccccgcac
    25561 cgttgctacc ggtgatttgt acccactctc ctgcgttcag cgtaaatgac aagccactaa
    25621 ataaggttcg ttcatcccgc tcgcaaagta actctctggc ttcaagcata cccacgcaat
    25681 aaccctgtta aaaacctggc tcgacttcgc gcatatcggg cagcttgtgc gctatccctt
    25741 tatggcaatc aatacaggtt tgcccatctt tcaccgcctg gtcatgcatc ttcgcggcaa
    25801 ccgatttctg ggcggttgta tccatatact cgaagttgtg acaattacgg cactcctgcg
    25861 agttgttgtc cttcatgcgc cgccactcat tctgtgccat cgtcaggcga tgagcttcaa
    25921 atttctgcgg cgtgtcaata acgccaaaaa ttttaccata cagctcttta cttgctttga
    25981 gcttgcgtat catctttggc acaaactcgt gcggaacgtg acaatccgga caggtcgcac
    26041 ggacgccgct acggttgttg tagtgcacgg aatccatgta ttcctgatac accgtgttac
    26101 gcatttcgtg gcagctaatg cagaactctt cggtattggc tttttccatc ccggtgttaa
    26161 agccgcccca gaagacgatg ccaccgacaa aaccgatcaa cagcagcgtc cccagcgcca
    26221 gacggctggg ggtacgccac catttccaca ggcgcttaat cagaccaggc ttacggtcag
    26281 aatttcccat aataacctct tatttcccgt aaccttttga cggggtaaag gtattcccca
    26341 cgattggcgc ggtatcggcc tgcggtacgt gacattgcag acagaaataa cgacgcggag
    26401 ccacttccgc gcccactttg ccgtcgctgt ccataaagtg agtaggacta atgcgcggcg
    26461 cgccagtggt gcgataactt tcgacaccgt ggcactgcaa gcagcggttg gtattggtcg
    26521 ttacctgata accttcaacg ctatgcggga tcattggcgg ctgattcaca tagttcagcg
    26581 gcatccgatc ctgctctttt ggcatccgaa tggccccttc ctgcgtcccg gatacttccg
    26641 gcgactggct aaaatcgacg ccattagccg cccaaaccgc gccgcttacc accagggcca
    26701 gcatcgccgt ccattgacac agcgctttct tcaggtcatg gcttttcatg atttcgctcc
    26761 cgaactccat cgtgtagtta ttgtaaatac atcctcagaa cagacatcca cgcagcgacc
    26821 gcacgtcatg caatcgcggc tggtgacctg caccgggctt tgttcatcca gcaccggggc
    26881 acgtagcaca tgcggttccg ggcaaacatg aaaacaatcc atacagcggt tacatttctg
    26941 ccgatcggtg gccgcaacgg taatcacacc tttgctaccc agcacgccat acagtgcacc
    27001 aaccggacaa atgtgcccgc accagccgtg ttcaacgacc agtagatcaa ataaaaacag
    27061 cgcgagaata agcagcgcgc cgctgccgaa gcccattacc aggctacgcc ccatcaggga
    27121 aacggggttg atccattccc agatgagcgt gccagttaag gccgatccca ccagaatgat
    27181 caccaacagc acgtagcgaa tgtggcgagg gatcgtcgca gactgattga ggtcaaatcg
    27241 tctgcgtaac caatttgcta agtcggtaat cgggttcagc gggcagaccc agctgcaaaa
    27301 taatcgcttt cctgccaggg cgtagagcac ggtgataatc accgcaccag tcagcgctac
    27361 cgtggcaggc agatgaccgc tggcaagact ttgcagtgtc atcagcggat cggtcagtgg
    27421 aacggtgtcg aataacaggc tactgctgta gttgccgtgc aagatccaca caccaaacca
    27481 cggaccgctc aaaaacatcc ccagcacgaa gaactgacaa aggcgacgta acactaacca
    27541 acggtgactg cgccaccagc cttttttctc cagcgcctcg cgcccggcgt cacgtttacg
    27601 atttgccatt gttcccctcc agccagccga agcggtaatg gtgacctaac tcccctttcg
    27661 ccagtgacag cggtaacacc ttgattgccg gttgttccag cacgcacact ttttcgcact
    27721 taccgcagcc ggtacaggcg tcgctgtgaa ccgtcggcag aaagcgggcg tgcttaccgg
    27781 ttcgcgtgtt gcgctccagc tccagggtga tggcctcatc aattttcggg cattcgcgat
    27841 aacaaacatc gcagcgcagc ccctgaaagt tgagacagtt ttcctggtcc accagtaccg
    27901 ccagccccat ccgcgcgtcg tcgatcgatt cgatctcacg atccagcgca ccgctcgggc
    27961 acactttggc gcacggaatg tcctcacaca tttcgcaagg aatatcccgt gcgacaaaat
    28021 atggcgtgcc cgccgacaga ccagaggcca gcgtcgccag ttttaaggtg tcgtaagggc
    28081 aagcctgaac acactgaccg caacgcacac aggcactggc aaaggcgttc tcgtttatgg
    28141 ctccgggcgg gcgcaaccgc acaccagatg cgcgtgcggt ttgctgttgt aaccccagcg
    28201 ccacacccac ggcagccagc ccgcctgctg tgcgaacgac atcgcgcaga aagcggcggc
    28261 gaccattttg aggtttcgct gaccgggaca taatggctta caccttctcc agtttgaccg
    28321 cgcacttctt gaagtccgtc tctttcgaga gcggatcggt cgcatccagc gtcagtttgt
    28381 taaccagctg tgcggcgtcg aagaacggca tgtacaccag gccctgcggc ggacggttac
    28441 gaccgcgcgt ttcgacaatc gagatcactt cgccacggcg agaaaccact ttcactttgt
    28501 cgccacggcg cagatcgcgc gctttcgcat ccagcgggtg aataaacaga accgcttccg
    28561 ggaaggcgcg gtgcagttcc ggtacacggc gagtcatact gccggtatgc cagtgctcca
    28621 ggacgcgtcc ggtagagagc cacaggtcgt actcttcatc cggcgcttcc gccgcaggtt
    28681 cgaacggcag cgcgaagatc accgctttgc catccggttt accgtagaac ttgtagcctt
    28741 cgcccgcttt cacgtacggg tcgttacctt cgctgtaacg ccactgcgtt tctttaccgt
    28801 tcaccaccgg ccagcgcaga ccgcgtgctt tgtggtaatc atcgaacggc gcgagatcgt
    28861 gaccgtgacc gcgaccaaac catgcgtact cttcgaacaa ccctttttgc agatagaagc
    28921 ccagctcgcg agattcgtcg ttcagctgat cttccgccag ttcggatacc gggaatttgc
    28981 tcacttccgg agtggcatac agaacctcat acagcgtttt gccacgcagt tccggtttct
    29041 tagccagcag ctcttccggc cacacttctt cagttttgaa gcgacgggag aactgaacta
    29101 actgccagag atccgatttt gcttcacccg gtgcctgtac ctgctgacgc cagaactgag
    29161 tacggcgttc ggcattaccg taagccccct ctttctctac ccacattgcg gtcggcagga
    29221 tcaagtccgc tgccagcgca ctgaccgtcg gatatggatc ggaaacgatg ataaagttgc
    29281 gcggatcgcg ccagcccggc atacgctctt cattaatgtt cggtccggcc tgcatgttat
    29341 tggtacacat ggtccagtaa acattcagtt tgccgtcttt cagcgcacgg tcttgtgcca
    29401 ccgcgtgcag accgattttc gccggaatgg tgccactcgg gatattccac ttcttctcgc
    29461 aaatatcacg atgtttctcg ttagtcacca ccatgtccgc aggcagacgg tgagcaaagg
    29521 tgcccacttc acgcgcagta ccacacgcgg aaggctgccc ggtcagggag aacggaccgc
    29581 aacccggctg ggaaattttg ccggtcagca ggtgcaggtt gtagaccagg ttgttggccc
    29641 acacaccacg ggtatgctgg ttgaagccca tcgtccagta ggagatgact ttcttgttcg
    29701 gatcggcata cagctgcgcc agttgttcta actggtcttt cggcacgccg gtcatttcgg
    29761 cggttttttc cagcgtatat tcagcaacga aggctttgta atcttcaaag ctcatcggtt
    29821 cggaggcgtc agaacccgga ttcttcgctg ctttttccag cggatgggtc ggacgtaaac
    29881 cgtagccgat gtccgtcgcc cctttgcgca ggttaacgtg tttgctgaag aagtcctgat
    29941 ttatcgcatt gttttgaatg atatagttgg cgatatagtt caggatcacc aggtcagatt
    30001 gcggcgtaaa gatgatgccg ttatccgcca gctcgaagct acgatgctgg taggtagaaa
    30061 gcaccgccac ggtaacgttc tggttagaga gacgacggtt agtgatgcgc gaccagagga
    30121 tcgggtgcat ctccgccatg tttgcgcccc acagcacaaa cgcgtcagcc tgctcgatgt
    30181 cgtcatagca gcccatcggc tcatccatac caaaggtacg cataaagcca actactgccg
    30241 acgccataca gtgacgcgcg ttcgggtcga tgttgttcga acggaagccc gctttaaaca
    30301 gcttggacgc ggcataacct tcccagatag tccactgacc agaaccgaac ataccgatcg
    30361 attccggccc tttttctttc agggcggttt tgaatttctc ttccatcaca tcgaaggcct
    30421 gatcccaggt gattggggta aattcgcctt ctttatcata tttaccgttt ttcatacgca
    30481 gcagcggctg cgtcaaacgg tctttaccgt acatgatttt gggcaggaaa tagcccttaa
    30541 tgcagttcag gccacggtta accggtgcgt ccgggtcgcc ctgacaagcc accacacgcc
    30601 cctgctgcgt tccgaccaga acgccgcaac ccgtaccgca gaaacggcac ggcgctttat
    30661 cccatttaat ggcttcctgc tgaccaacaa cggcgcgggc aacgcccggc acgctgagac
    30721 cggcagccgc cgcagcggcc gcaacggcgt tagctttcat aaagctacga cgactgagtt
    30781 tcatggtgtt tcctcacctt gctcttcctg ctggtgataa accagcgaca ccgccagcac
    30841 gccctctacg ttgcgtactg actcaatggt ttggatcagc gtttcgctgt cttctgcttc
    30901 caccaccaca atcaactgac cgctcggcgc gtcgctgaca gcaacttcac agccggtaaa
    30961 ggcgttcagt tgggtgctga tgtctgaaat tcgttcgctt ttggcctgca cgaccaggct
    31021 gcaaacttgc cagttagtgt gcatggagat actccgcagt tatggctgat accggacagc
    31081 tggctgcaca tgctccacat ccgttgcaaa gttggctatt aagttgcggc tggtagatcc
    31141 cggagagcgt cgggcgaaag ataattgcca ttggctcaca gctatcctga cagcggcggc
    31201 attcaactga ctgatacgcc aggcacgcgt ccccaatggt gaactgcaaa tcccaggccc
    31261 tggtgtggcg cggagaaaat agcgattcgg ggcaagcctg cgcgcaggcg taacagaagc
    31321 tgcactcgtt atttttgaaa ttaacgctcg gatagctacc cgcgccgcgt tgcagaatgt
    31381 tgttttcaca agcattaata caggcgtcgc agcgggtgca atgggtcaga aaatgagatt
    31441 catcaccgga ccagggcgga cggataccgt tactggcttt gcgccagcga ccagtgagta
    31501 tgccccgacg ggatgcatca atcttcacat tgaccttcca tcattaacgc gctctgaaaa
    31561 ttgagagcga ccaaataaac cgcataatta ataagccatt tttatagccg ctaagatatt
    31621 aaaggatgtg tcaaagatgc ataccccgat cggggtaaaa cctgtagcag gatcaaaaga
    31681 gtggcgggaa gcgtggcaaa aacgggcttt tgctcacatt tcaaatggtt ataaacatat
    31741 ttatatagcg attaattcgc cagagatatt tctgctggt
//
LOCUS       DAHHTG010000049        31291 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.049, whole genome shotgun sequence.
ACCESSION   DAHHTG010000049 DAHHTG010000000
VERSION     DAHHTG010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 31291)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31291)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31291
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.049"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            150..3908
                     /gene="yfaL"
                     /locus_tag="NBI74_002178"
     CDS             150..3908
                     /gene="yfaL"
                     /locus_tag="NBI74_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA-I family autotransporter adhesin YfaL/EhaC"
                     /protein_id="HCD1932511.1"
                     /translation="MRIISLRKEYLSLLPSMIASLFSANGAAAVIDSCQGYDIKASCQ
                     ASRQSLSGNIQDWSIADGQWLIFSGMVNNASGGAVFLQHSAEFTISPQNETGMTLFAD
                     NSVSGEYNNGGAIFAKENSTINLANVIFDSNVAGGYGGAIYSAGTNDTGAADLRVTNA
                     VFHNNIANDGKGGAIYTINNDVYLSDDAFNNNQAYTSTSYSDGDGGAIDVTDNSTDKT
                     HLSGKTIINNTSFTNNYAEGYGGAIYTSSTTSPYLIDISVDDNYDQNNGVMIDENNSA
                     SGYDHSATAAAGGFMYIGHSVAEFNIAADKTLVIGNTSNDGAIDSLAGTGVIVKEGAG
                     ELVLNADNNAFTGEMSIQNGEVTLGRSDELMNVGDTHCQSDPQDCFGLMVGSTVHSEY
                     QAELNVGNTQQTFVHSLTGFANGILNIDAGGNVTVNQGGFSGSIQGEGQLTVAQDGSY
                     LLTGAQSMALTGDIVVEDNAVLSLAGNQADLRAMQSDPQSIVLNGGVLDLSDFTTWDG
                     DSSYNDGLQISGSGGTVIGSNDVVDISSGDDLHIGGSDASQNGVYVVINAGDQRVTLA
                     NNNGYLGNTQIASGTLEVSDNSQLGDTSYNRSVIFTDPQQHSEMDVTTDVDTRSATTG
                     QGRNIEMRADGEIHVEDGVDTQWGGLMADSTGQQLDSVSTLTKSGGGTLELTASGTAT
                     SAVRVEDGTLKGEAENIIPYVSSLWVGEDGVFETGQNQDIRSIDATSGGDIDITDGTV
                     LRLTQQDTNQALDASLFSGDGTLVNATDGVTLTGELNTNLETDSLTYLSDVTVNGNLT
                     NTSGAVSLQNGIAGDTLTVNGDYTGGGTLLLDSELNGDDSASDQLVLNGNTAGNTTVV
                     INPITGIGEPTSTGIKVVDFAADPTQFQNNAQFSLAGSGYVNMGAYDYTLVEDNNDWY
                     LRSQEVNPTPPPDPDPTPDPDPTPDPDPTPDPEPTPAYQPVLNAKVGGYFNNLRAANQ
                     AFVMERRDHAGGDGQTLNLRVIGGRYHYTAAGQLAQHEDTSTVQLSGNLFSGHWGDDG
                     EWMLGIVGGYSDNQGDSRSNMTGTRADNQNRGYAVGLTSSWYQHGNQKQGAWLDSWLQ
                     YAWFNNDVSEQDDGVDHYHSSGIIASLEAGYQWLPGRGVVIEPQAQVIYQGVQQDDFT
                     AANHARVSQSQGDDIQMRLGLHSEWRTAVGVTPTLDLNYYHDPHATEIEEDGSTISDD
                     AVKQRGEIKVGITGNISQRVSLRGSVAWQKGSDDFAQMAGFLSMTVKW"
     gene            complement(4048..4770)
                     /locus_tag="NBI74_002179"
     CDS             complement(4048..4770)
                     /locus_tag="NBI74_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-demethylubiquinone
                     3-O-methyltransferase/2-octaprenyl-6-hydroxy phenol
                     methylase"
                     /protein_id="HCD1932512.1"
                     /translation="MNAEKSPVNHNVDHEEIAKFEAVASRWWDLEGEFKPLHRINPLR
                     LGYIAERAGGLFGKKVLDVGCGGGILAESMAREGATVTGLDMGFEPLQVAKLHALESG
                     IQVDYVQETVEEHAAKHAGQYDVVTCMEMLEHVPDPQSVVRACAQLVKPGGDVFFSTL
                     NRNGKSWLMAVVGAEYILRMVPKGTHDVKKFIKPAELLGWVDQTSLKERHMTGLHYNP
                     ITNTFKLGPGVDVNYMLHTQNK"
     gene            4917..7544
                     /gene="gyrA"
                     /locus_tag="NBI74_002180"
     CDS             4917..7544
                     /gene="gyrA"
                     /locus_tag="NBI74_002180"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit A"
                     /protein_id="HCD1932513.1"
                     /translation="MSDLAREITPVNIEEELKSSYLDYAMSVIVGRALPDVRDGLKPV
                     HRRVLYAMNVLGNDWNKAYKKSARVVGDVIGKYHPHGDLAVYDTIVRMAQPFSLRYML
                     VDGQGNFGSIDGDSAAAMRYTEIRLAKIAHELMADLEKETVDFVDNYDGTEKIPDVMP
                     TKIPNLLVNGSSGIAVGMATNIPPHNLTEVINGCLAYIDDEDISIEGLMEHIPGPDFP
                     TAAIINGRRGIEEAYRTGRGKVYIRARAEVEVDAKTGRETIIVHEIPYQVNKARLIEK
                     IAELVKEKRVEGISALRDESDKDGMRIVIEVKRDAVGEVVLNNLYSQTQLQVSFGINM
                     VALHHGQPKIMNLKDIIAAFVRHRREVVTRRTIFELRKARDRAHILEALAVALANIDP
                     IIELIRHAPTPAEAKTALVANPWQLGNVAAMLERAGDDAARPEWLEPEFGVRDGLYYL
                     TEQQAQAILDLRLQKLTGLEHEKLLDEYKELLDQIAELLRILGSADRLMEVIREELEL
                     VREQFGDKRRTEITANSADINLEDLITQEDVVVTLSHQGYVKYQPLSEYEAQRRGGKG
                     KSAARIKEEDFIDRLLVANTHDHILCFSSRGRVYSMKVYQLPEATRGARGRPIVNLLP
                     LEQDERITAILPVTEFEEGVKVFMATANGTVKKTVLTEFNRLRTAGKVAIKLVDGDEL
                     IGVDLTSGEDEVMLFSAEGKVVRFKESSVRAMGCNTTGVRGIRLGEGDKVVSLIVPRG
                     DGAILTATQNGYGKRTAVAEYPTKSRATKGVISIKVTERNGLVVGAVQVDDCDQIMMI
                     TDAGTLVRTRVSEISIVGRNTQGVILIRTSEDENVVGLQRVAEPVDEEDLDTIDGSAA
                     EGDDEIAPEVDVDDEPEEE"
     gene            7693..9381
                     /locus_tag="NBI74_002181"
     CDS             7693..9381
                     /locus_tag="NBI74_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416733.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2138 domain-containing protein"
                     /protein_id="HCD1932514.1"
                     /translation="MSGEKKAKGWRFYGLVGFGAIALLSAGVWALQYSGSGPEKTLSP
                     LVVHNNLQIDLNEPDLFLDSDSLSQLPKDLLTIPFLHDVLSEDFVFYYQNHADRLGIE
                     GSIRRIVYEHDLTLKDKLFSSLLDQPAQAALWHDKQGHLSHYMVLIQRSGLSKLLEPL
                     LFAATSDSQLSKTEISSIKINSETIPVYQLRYNGNNVLMFATYQDKILAFSSSDMLFK
                     DDQQDTEATAIASDLLSGKKRWETRFGLEARTAEKTPVRQRIVVSARLLGFGYQRLMP
                     SFAGVRFEMGNDGWHSFLALNDESASVDASFDFTPVWNSMPAGASFCVAVPYSHGIAE
                     EMLSHISQENDKLNGALDGGAGLCWYEDSKLQTPLFVGQFDGTAEQAQLPGKLFTQNI
                     GAHESKAPEGVLPVSQTQQGEAQIWRREVSSRYGQYPKAQAAQPDQLMSDYFFRVSLA
                     MQNKTLLFSLDDTLVNNALQTLNKTRPAMVDVIPTDGIVPLYINPQGMAKLLRNETLT
                     SLPKNLEPVFYNAAQTLLMPKLDALSQQPRYVMKLAQMEPGVAWQWLPITWQPL"
     gene            9378..10001
                     /locus_tag="NBI74_002182"
     CDS             9378..10001
                     /locus_tag="NBI74_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311139.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1175 domain-containing protein"
                     /protein_id="HCD1932515.1"
                     /translation="MRHGLLALICWLYCVVAHSEMLNVEQSGLFRAWFVRIAQEQLRQ
                     GPSPRWYQQDCAGLVRFAVNEALKVHDSKWLKSNGLSNQYLPPEMTLTPEQRQLAQNW
                     NQGNGKTGPYVTAINLIQYNSQFIGQDINQALPGDMIFFDQGDAQHLMVWMGRYIIYH
                     TGSATKTDNGMRAVSLQQLMTWKDTRWIPNDSNPNFIGIYRLNFLAR"
     gene            10025..14539
                     /locus_tag="NBI74_002183"
     CDS             10025..14539
                     /locus_tag="NBI74_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000536590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HCD1932516.1"
                     /translation="MGTGLANAEDSLPSSNYVPPAGGTFFLLADSSFSSSEEAKVRLE
                     APGRDYRRYQMEEYGGVDVRLYRIPDPMAFLRQQKNLHRIVVQPQYLGDGLNNTLTWL
                     WDNWYGKSRRVMQRTFSSQSRQNVTQALPELQLGNAIVKPSRYVQNSQFSPLKKYPLV
                     EQFRYPLWQAKPVEPQQGVKLEGASSNFISPQPGNIYIPLGKQEPGLYLVEAMVGGYR
                     ATTVVFVSDTVALSKVSGNELLVWTAGKKQGEAKPGSEILWTDGLGVMTRGVTDGSGT
                     LQLQHISPERSYILGKDAEGGVFVSENFFYESEIYNTRLYIFTDRPLYRAGDRVDVKV
                     MGREFHDPLHSSPIVSAPAKLSVLDANGSLLQTVNVTLDARNGGQGSFRLPENAVAGG
                     YELRLAYRKQVYSSSFRVANYIKPHFEIGLALDKKEFKTGEAVSGKLQLLYPDGEPVK
                     DARVQLSLRAQQLSMVGNDLRYAGRFPVSMEGSETVSDDNGHVALNLPAADKPSRYLL
                     TVSASDGAAYRVTTTKEILIERGLAHYSLSTAAQYSNSGESVVFRYAALESSKQVPVT
                     YEWLRLEDRTSHSGELQSGGKSFTVNFATPGNYNLTLRDKDGLILAGLSHAVSGKDST
                     SHSGTVDIVADKTLYQPGETAKMLITFPEPIDEALLTLERDRVEQQSLLSHPANWLTL
                     QRLNDTQYEARVPVSNSFAPNITFSVLYTRNGQYSFQNAGIKVAVPQLDIRVKTDKTH
                     YQPGELVNVELTSSLKGKPVSAQLTVGVVDEMIYALQPEIAPNIGKFFYPLGRNNVRT
                     SSSLSFISYDQALSSEPVAPGATNRSERRVKMLERPRREEVDTAAWMPSLTTDKQGKA
                     YFTFLMPDSLTRWRITARGMNGDGLVGQGRAYLRSEKNLYMKWSMPTVYRMGDKPAAG
                     LFIFSQQDNDPVALVTKFAGAEMRQTLTLHKGANYISLTQNIQQSGLLSAELQQNGQV
                     QDSISTKLSFVDNRWPVEQQKNVMLGGGDNALMLPEQASNIRLQSSETPQEIFRNNLD
                     ALVDEPWGGVINTGSRLIPLSLAWRSLADHQSAAANDIRQMIQDNRLRLMQLAGPGAR
                     FTWWGEDGNGDAFLTAWAWYADWQASQALGVTQQPEYWQHMLDSYAEQADNMPLLHRA
                     LVLAWAQEMNLPCKTLLKGLDEAIARRGTKTEDFSEEDTSDINDSMILDTPESPLADA
                     VANVLTMTLLKKAQLKSTVMPQVQQYAWDKAANSNQPLAHTVVLLNSGGDATQAAAIL
                     SGLTAEQSTIERALAMNWLAKYMATMPPVVLPAPAGAWAKHKLTGGGEYWRWVGQGVP
                     DILSFGDELPPQNVQVRWREPAKTAQQSNIPVTVERQLYRLIPGEEEMSFTLQPVTSN
                     EIDSDALYLDEITLTSEQDAVLRYGQVEVPLPPGADVERTTWGISVNKPNAGKQQGQL
                     LEKARNEMGELAYMVPVKELTGTVTFRHLLRFSQKGQFVLPPARYVRSYAPAQQSVAP
                     GSEWIGMQVK"
     gene            14540..16189
                     /locus_tag="NBI74_002184"
     CDS             14540..16189
                     /locus_tag="NBI74_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaQ family protein"
                     /protein_id="HCD1932517.1"
                     /translation="MNWYRIVWLLALVTLPTLAEETPLQLALRGAQHDQLYQLSSSGV
                     TKVSVLPDTLTTPLGSLWKLYVYAWLEDTHQPEQPYQCRGNSPEEVYCCQAGESITRD
                     SALVRSCGLYFAPQRLHIGADMWGQYWQQRQAPAWLASLTTLKPETSVTVKSLLDSLA
                     TLPAQNKAQEVLLDVVLDEAKIGVASMLGSRVRVKTWSWFADDKQEIRQGGFAGWLTD
                     GTPLWVTGSGTSKTVLTRYATALNRVLPVPTQVASGQCVLVDLFARYPLKKVTEEKST
                     TAVKPGVLNGRYRVTFANGNHMTFVSHGETTLLTVKGKLKLQSHLDREEYVARVLDRE
                     AKSTPPEAAKAMTVAIRTFLQQNADREGDCLTIPDSSATQRVSASPATTGARTMTAWT
                     QDLIYAGDPVHYHGSRATEGTLSWRHATAQAGQGERYDQILAFAYPDNSLSRWGAPRS
                     TCQLLPKAKAWLAKKMPQWRRILQGETGYNEPDVFAVCRLVSGFPYTDRQQKRLFIRN
                     FFTLQDRLDLTHEYLHLAFDGYPTGLDENYIETLTRQLLMD"
     gene            16194..16970
                     /locus_tag="NBI74_002185"
     CDS             16194..16970
                     /locus_tag="NBI74_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaP family protein"
                     /protein_id="HCD1932518.1"
                     /translation="MRKIFLPLLLVALSPVAHSEGVQEVEIDAPLSGWHPAEGEDASF
                     SQTINYPASSVNMADDQNISAQIRGKIKNYAAAGKVQQGRLVVNGASMPQRIESDGSF
                     ARPYIFTEGSNSVQVISPDGQSRQKMQFYSTPGAGAIRARLRLVLSWDTDNTDLDLHV
                     VTPDGEHAWYGNTVLKNSGALDMDVTTGYGPEIFAMPAPVHGRYQVYINYFGGRSETE
                     LTTAQLTLITDEGSVNEKQETFIVPMRNAGELTLVKSFDW"
     gene            complement(17044..18228)
                     /gene="atoB"
                     /locus_tag="NBI74_002186"
     CDS             complement(17044..18228)
                     /gene="atoB"
                     /locus_tag="NBI74_002186"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA acetyltransferase"
                     /protein_id="HCD1932519.1"
                     /translation="MKNCVIVSAVRTAIGSFNGSLASTSAIDLGATVIKAAIERAKID
                     SLHVDEVIMGNVLQAGLGQNPARQALLKSGLAETVCGFTVNKVCGSGLKSVALAAQAI
                     QAGQAQSIVAGGMENMSLAPYLLDAKARSGYRLGDGQVYDVILRDGLMCATHGYHMGI
                     TAENVAKEYGITREMQDELALHSQRKAAAAIESGAFTAEIVPVNVVTRKKTFVFSQDE
                     FPKADSTAEALGALRPAFDKAGTVTAGNASGINDGAAALVIMEESAALAAGLNPLARI
                     KSYASGGVPPALMGMGPVPATQKALQLAGLQLADIDLIEANEAFAAQFLAVGKTLGFD
                     PEKVNVNGGAIALGHPIGASGARILVTLLHAMQARDKTLGLATLCIGGGQGIAMVIER
                     LN"
     gene            complement(18259..19581)
                     /locus_tag="NBI74_002187"
     CDS             complement(18259..19581)
                     /locus_tag="NBI74_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00366 family protein"
                     /protein_id="HCD1932520.1"
                     /translation="MIGRISRFMTRFVSRWLPDPLIFAMLLTLLTFVIALWLTPQTPI
                     SMVKMWGDGFWNLLAFGMQMALIIVTGHALASSAPVKSLLRTAASAAKTPVQGVMLVT
                     FFGSVACVINWGFGLVVGAMFAREVARRVPGSDYPLLIACAYIGFLTWGGGFSGSMPL
                     LAATPGNPVEHIAGLIPVGDTLFSGFNIFITVALIVVMPFITRMMMPKPSDVVSIDPK
                     LLMEEADFQKQLPKDAPPSERLEESRILTLIIGALGIAYLAMYFSEHGFNITINTVNL
                     MFMIAGLLLHKTPMAYMRAISAAARSTAGILVQFPFYAGIQLMMEHSGLGGLITEFFI
                     NVANKDTFPVMTFFSSALINFAVPSGGGHWVIQGPFVMPAAQALGADLGKSVMAIAYG
                     EQWMNMAQPFWALPALAIAGLGVRDIMGYCITALLFSGVIFVIGLTLF"
     gene            complement(19578..20228)
                     /locus_tag="NBI74_002188"
     CDS             complement(19578..20228)
                     /locus_tag="NBI74_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-CoA--3-ketoacid-CoA transferase"
                     /protein_id="HCD1932521.1"
                     /translation="MDAKQRIARRVAQELRDGDIVNLGIGLPTMVANYLPEGIHITLQ
                     SENGFLGLGPVTTAHPDLVNAGGQPCGILPGAAMFDSAMSFALIRGGHIDACVLGGLQ
                     VDEEANLANWVVPGKMVPGMGGAMDLVTGSRKVIIAMEHCAKDGSAKILRRCTMPLTA
                     QHAVHMLVTELAVFRFIDGKMWLTEIADGCDLATVRAKTEARFEVAADLNTQRGDL"
     gene            complement(20228..20890)
                     /gene="atoD"
                     /locus_tag="NBI74_002189"
     CDS             complement(20228..20890)
                     /gene="atoD"
                     /locus_tag="NBI74_002189"
                     /EC_number="2.8.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate CoA-transferase subunit alpha"
                     /protein_id="HCD1932522.1"
                     /translation="MKTKLMTLQDATGFFRDGMTIMVGGFMGIGTPSRLVEALLESGV
                     RDLTLIANDTAFVDTGIGPLIVNGRVRKVIASHIGTNPETGRRMISGEMDVVLVPQGT
                     LIEQIRCGGAGLGGFLTPTGVGTVVEEGKQTLTLDGKTWLLERPLRADLALIRAHRCD
                     TLGNLTYHLSARNFNPLIALAADITLVEPDELVETGELQPDHIVTPGAVIDHIIVSQE
                     SK"
     gene            complement(21086..22471)
                     /gene="atoC"
                     /locus_tag="NBI74_002190"
     CDS             complement(21086..22471)
                     /gene="atoC"
                     /locus_tag="NBI74_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetate metabolism transcriptional
                     regulator AtoC"
                     /protein_id="HCD1932523.1"
                     /translation="MTAINRILIVDDEDNVRRMLSTAFALQGFETHCANNGRTALHLF
                     ADIHPDVVLMDIRMPEMDGIKALKEMRSHETRTPVILMTAYAEVETAVEALRCGAFDY
                     VIKPFDLDELNLIVQRALQLQSMKKEIRHLHQALSTSWQWGHILTNSPAMMDICKDTA
                     KIALSQASVLISGESGTGKELIARAIHYNSRRAKGPFIKVNCAALPESLLESELFGHE
                     KGAFTGAQTLRQGLFERANEGTLLLDEIGEMPLVLQAKLLRILQEREFERIGGHQTIK
                     VDIRVIAATNRDLQAMVKEGTFREDLFYRLNVIHLILPPLRDRREDISLLANHFLQKF
                     SSENQRDIIDIDPMAMSLLTAWSWPGNIRELSNVIERAVVMNSGPIIFSEDLPPQIRQ
                     PVCNAGEAKTAPVGERNLKEEIKRVEKRIIMEVLEQQEGNRTRTALMLGISRRALMYK
                     LQEYGIDPADV"
     gene            complement(22468..24294)
                     /gene="atoS"
                     /locus_tag="NBI74_002191"
     CDS             complement(22468..24294)
                     /gene="atoS"
                     /locus_tag="NBI74_002191"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     AtoS"
                     /protein_id="HCD1932524.1"
                     /translation="MHYMKWIYPRRLRNQMILMAILMVIVPTLTIGYIVETEGRSAVL
                     SEKEKKLSAVVNLLNQALGNRYDLYIDLPREERIRALNAELAPITENITHAFPGIGAG
                     YYNKTLDAIITYAPSALYQNNVGVTIAADHPGREVMRTNTPLVYSGRQVRGDILNSMI
                     PIERNGEILGYIWANELTEDIRRQAWKMDVRIIIVLTAGLLISLLLIVLFSRRLSANI
                     DIITDGLSTLAQNIPTRLPQLPGEMGQISQSVNNLAQALRETRTLNDLIIENAADGVI
                     AIDRQGDVTTMNPAAEVITGYQRHELVGQPYSMLFDNTQFYSPVLDTLEHGTEHVALE
                     ISFPGRDRTIELSVTTSRIHNTHGEMIGALVIFSDLTARKETQRRMAQAERLATLGEL
                     MAGVAHEVRNPLTAIRGYVQILRQQTRDPIHQEYLSVVLKEIDSINKVIQQLLEFSRP
                     RHSQWQQVSLNALVEETLVLVQTAGVQARVDFISELDNELSPINADRELLKQVLLNIL
                     INAVQAISARGKIRIRTWQYSDSQQAISIEDNGSGIDLSLQKKIFDPFFTTKASGTGL
                     GLALSQRIINAHQGDIRVASLPGYGATFTLILPINPQGNQTV"
     gene            24461..27310
                     /gene="rcsC"
                     /locus_tag="NBI74_002192"
     CDS             24461..27310
                     /gene="rcsC"
                     /locus_tag="NBI74_002192"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RcsC"
                     /protein_id="HCD1932525.1"
                     /translation="MKYLASFRTTLKASRYMFRALALVLWLLIAFSSVFYIVNALHQR
                     ESEIRQEFNLSSDQAQRFIQRTSDVMKELKYIAENRLSAENGVLSPRGRETQTDVPAF
                     EPLFADSDCSAMSNTWRGSLESLAWFMRYWRDNFSAAYDLNRVFLIGSDNLCMANFGL
                     RDMPVERDTALKALHERINKYRNAPQDDSGSNLYWISEGPRPGVGYFYALTPVYLANR
                     LQALLGVEQTIRMENFFLPGTLPMGVTILDENGHTLISLTGPESKIKGDPRWMQERSW
                     FGYTEGFRELVLKKNLPPSSLSIVYSVPVDKVLERIRMLILNAILLNVLAGAALFTLA
                     RMYERRIFIPAESDALRLEEHEQFNRKIVASAPVGICILRTADGVNILSNELAHTYLN
                     MLTHEDRQRLTQIICGQQVNFVDVLTSNNTNLQISFVHSRYRNENVAICVLVDVSSRV
                     KMEESLQEMAQAAEQASQSKSMFLATVSHELRTPLYGIIGNLDLLQTKELPKGVDRLV
                     TAMNNSSSLLLKIISDILDFSKIESEQLKIEPREFSPREVMNHITANYLPLVVRKQLG
                     LYCFIEPDVPVALNGDPMRLQQVISNLLSNAIKFTDTGCIVLHVRADGDYLSIRVRDT
                     GVGIPAKEVVRLFDPFFQVGTGVQRNFQGTGLGLAICEKLISMMDGDISVDSEPGMGS
                     QFTVRIPLYGAQYPQKKGVEGLSGKRCWLAVRNASLCQFLETSLQRSGIVVTTYEGQE
                     PTPEDVLITDEVVNKKWQGRAVVTFCRRHIGIPLEKAPGEWVHSVAAPHELPALLARI
                     YLIEMESDDPANALPSTDKAVSDNDDMMILVVDDHPINRRLLADQLGSLGYQCKTAND
                     GVDALNVLNKNHIDIVLSDVNMPNMDGYRLTQRIRQLGLTLPVIGVTANALAEEKQRC
                     LESGMDSCLSKPVTLDVIKQTLTVYAERVRKSRES"
     gene            complement(27510..28160)
                     /gene="rcsB"
                     /locus_tag="NBI74_002193"
     CDS             complement(27510..28160)
                     /gene="rcsB"
                     /locus_tag="NBI74_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RcsB"
                     /protein_id="HCD1932526.1"
                     /translation="MNNMNVIIADDHPIVLFGIRKSLEQIEWVNVVGEFEDSTALINN
                     LPKLDAHVLITDLSMPGDKYGDGITLIKYIKRHFPSLSIIVLTMNNNPAILSAVLDLD
                     IEGIVLKQGAPTDLPKALAALQKGKKFTPESVSRLLEKISAGGYGDKRLSPKESEVLR
                     LFAEGFLVTEIAKKLNRSIKTISSQKKSAMMKLGVENDIALLNYLSSVTLSPADKD"
     gene            complement(28177..30849)
                     /gene="rcsD"
                     /locus_tag="NBI74_002194"
     CDS             complement(28177..30849)
                     /gene="rcsD"
                     /locus_tag="NBI74_002194"
                     /EC_number="2.7.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase RcsD"
                     /protein_id="HCD1932527.1"
                     /translation="MRQKETTATTRFSLLPGSITRFFLLLIIVLLVTMGVMVQSAVNA
                     WLKDKSYQIVDITHAIQKRVDTWRYVTWQIYDNIAATTSPSSGEGLQETRLKQDVYYL
                     EKPRRKTEALIFGSHDNSTLEMTQRMSTYLDTLWGAENVPWSMYYLNGQDNSLVLIST
                     LPLKDLTSGFKESTVSDIVDSRRAEMLQQANALDERESFSNMRRLAWQNGHYFTLRTT
                     FNQPGHLATVVAFDLPINDLIPPGMPLDSFRLEPDATATGNNENEKEGTDSVSIHFNS
                     TKIEISSALNSTDMRLVWQVPYGTLLLDTLQNILLPLLLNIGLLALALFGYTTFRHFS
                     SRSTESVPSTAVNNELRILRAINEEIVSLLPLGLLVHDQESNRTVISNKIADHLLPHL
                     NLQNITTMAEQHQGIIQATINNELYEIRMFRSQVAPRTQIFIIRDQDREVLVNKKLKQ
                     AQRLYEKNQQGRMTFMKNIGDALKEPAQSLAESAAKLNAPEGKQLANQADVLVRLVDE
                     IQLANMLADDSWKSETELFSVQDLIDEVVPSVLPAIKRKGLQLLINNHLKAHDMRRGD
                     RDALRRILLLLMQYAVTSTQLGKITLEVDQDESSEDRLTFRILDTGEGVSIHEMDNLH
                     FPFINQTQNDRYGKADPLAFWLSDQLARKLGGHLNIKTRDGLGTRYSVHIKMLAADPE
                     VEEEEERLLDDVCVMVDVTSAEIRNIVTRQLENWGATCITPDERLISQDYDIFLTDNP
                     SNLTASGLLLSDDESGVREIGPGQLCVNFNMSNAMQEAVLQLIEVQLAQEEVTESPLG
                     GDENAQLHASGYYALFVDTVPDDVKRLYTEAATSDFAALAQTAHRLKGVFAMLNLVPG
                     KQLCETLEHLIREKDVPGIEKYISDIDSYVKSLL"
BASE COUNT         7700 a         7633 c         8401 g         7557 t
ORIGIN      
        1 ttaatctgaa ttgtgatgca tctaaattaa tcatcaaata ataacaaagt ggttattaca
       61 tcgggggcag aatttacaaa agaaaaatac cctatatcgc ataatgcgat taaatacgcc
      121 atcttataga aaatgatggt tagatgaata tgcggattat ctctctacgc aaggagtatt
      181 tatctttact cccgtcaatg attgcatctc ttttctctgc taacggtgcc gcagcggtca
      241 ttgattcatg ccagggatat gatatcaaag cgagctgtca ggccagcagg caaagccttt
      301 cgggtaatat acaggactgg agcatcgcgg atggacaatg gttgattttt tcgggtatgg
      361 tcaataatgc cagcggtggc gcagtatttt tgcagcacag tgctgaattt acgatatcac
      421 cacaaaatga aacagggatg accctgtttg ctgataactc ggttagtggc gaatataata
      481 atggcggggc tatctttgct aaagaaaact caacgataaa tcttgcgaat gttattttcg
      541 acagtaatgt tgcaggaggc tatggcggag caatctattc tgccggtacc aatgataccg
      601 gtgccgccga tttacgagtc actaacgccg tatttcacaa taacatcgct aatgacggca
      661 aaggcggtgc aatttatacc atcaataatg acgtctattt aagtgatgat gcttttaata
      721 ataaccaggc atatacatca acaagttata gtgatgggga tggcggcgcc attgatgtta
      781 cggataatag tacggataag acacatcttt caggaaaaac gataattaat aatacctcct
      841 ttaccaataa ctatgcagaa ggttatggcg gagcaattta taccagcagc accacatccc
      901 cgtatcttat tgatatttct gttgatgata attatgacca aaacaatggt gtgatgattg
      961 atgaaaataa cagcgcctcg ggatatgacc attcggcgac ggcagcggcg gggggcttta
     1021 tgtatatcgg tcatagtgtg gctgaattta acattgccgc cgataaaacg ctggtaatag
     1081 gcaataccag caacgatggc gcgattgatt ctcttgccgg aacgggagtc atcgtgaaag
     1141 agggagcggg agagttagtc ctcaatgccg acaataatgc gtttaccggc gagatgagta
     1201 tccagaatgg cgaggtgacg ttggggcgca gcgatgagtt aatgaatgtc ggcgacacgc
     1261 actgtcagag cgatccgcag gattgctttg gtctgatggt cggcagcact gttcattctg
     1321 agtatcaggc agaactgaat gttggcaata cacaacaaac gtttgtgcac tcattaaccg
     1381 gttttgctaa tggcattttg aatatcgacg caggcggcaa tgttaccgta aaccagggcg
     1441 gtttttccgg ctcgattcag ggtgaaggac agttgacggt agcgcaagat ggcagctatc
     1501 tcctgacagg ggcgcagtcg atggcattaa ccggcgatat tgtggtggaa gataacgccg
     1561 tattgtcgct ggcggggaat caggccgatt tacgggcaat gcagagcgat ccgcaatcga
     1621 tcgtgctaaa tggcggagta ttggatctct ctgatttcac cacctgggat ggcgatagct
     1681 catacaatga cggtctgcaa atcagcggca gcggggggac agtgattggc agtaatgatg
     1741 tcgttgatat cagcagtggc gatgatttac atattggcgg cagtgatgcc agccagaatg
     1801 gcgtttatgt cgtcattaat gcgggggatc agcgcgtaac gctggcgaat aacaacggtt
     1861 atctcggtaa tacgcaaatc gcctccggta cgcttgaagt cagcgataat tcgcaattgg
     1921 gggacacaag ttacaaccgt tcagtgatct tcacagatcc ccaacaacac agtgaaatgg
     1981 atgtgacgac tgatgttgac actcgctcgg ctacaacagg tcagggcagg aatattgaaa
     2041 tgcgcgctga tggcgaaata cacgttgagg atggcgtaga tacgcaatgg ggcgggttga
     2101 tggcggatag taccgggcaa caactggata gcgtaagcac gttgactaaa agtggcggcg
     2161 ggacgctgga gttgaccgcc agcggcactg cgacgtctgc ggtacgtgtg gaagacggaa
     2221 cgctaaaagg tgaagcggag aatattattc cttatgtttc ttcactgtgg gtgggggaag
     2281 acggtgtttt tgaaaccggg caaaatcagg atattcgctc aatcgatgcc acctctggcg
     2341 gcgatatcga cataaccgat ggtactgtgt tgcgattgac gcagcaggat acgaaccagg
     2401 cgctggatgc ctcgctgttt agcggcgacg gtacgctggt gaatgccacc gatggcgtga
     2461 cgttgacagg tgagcttaat accaaccttg aaactgacag cctgacttat ctttccgacg
     2521 tgacggttaa tggcaatctg accaatacgt ccggtgctgt cagtctgcaa aatggcatcg
     2581 ctggcgacac gctgacggta aatggtgatt ataccggcgg cggtacgcta ctgctcgaca
     2641 gcgaattaaa cggcgatgac tcggcaagcg accagttggt gttgaacggt aatactgctg
     2701 gcaataccac ggttgtgatt aatcccatta cagggattgg tgagccgaca tctacaggta
     2761 ttaaagtggt tgatttcgca gctgatccca cgcagtttca aaacaatgcg cagttcagtc
     2821 tggcaggaag cggctacgtc aatatggggg cgtatgacta cacgctggtg gaagataaca
     2881 acgactggta tctgcgatca caagaagtca atccgacgcc gccgcctgat ccagacccga
     2941 ctccagatcc tgatcccacg ccggatcccg acccaacccc cgaccctgaa cctacgcctg
     3001 cttaccagcc ggtgttgaat gccaaagttg gtggatattt taataaccta cgtgcggcaa
     3061 atcaggcatt tgtgatggag cgacgcgatc acgcaggtgg cgatggtcag acgctgaatt
     3121 tacgcgttat cggcgggcgt tatcattaca cggcggcagg gcagttggct cagcatgaag
     3181 acacttctac ggtgcagctt agcggcaacc tgtttagcgg gcactggggt gatgatggcg
     3241 agtggatgct tgggattgtt ggtggctaca gcgataacca gggcgacagc cgctcgaata
     3301 tgaccggaac tcgcgccgat aaccagaacc gcggttatgc cgttgggctg acctcaagct
     3361 ggtatcagca cggtaatcag aagcaggggg cctggttgga tagctggctg caatatgcgt
     3421 ggtttaacaa tgatgtttcc gaacaagacg atggagtgga tcattaccac tcgtcgggga
     3481 ttatcgcctc gctggaagcc ggttatcagt ggttaccggg gcgtggtgtg gtgattgaac
     3541 cgcaggcgca ggtgatttat cagggcgtgc agcaggatga ttttaccgcc gctaaccatg
     3601 cgcgcgtgtc acaatcgcag ggtgatgata ttcagatgcg gttgggttta cacagcgaat
     3661 ggcgtactgc tgttggtgtc acgccaacgt tagatctgaa ttattatcac gatccccatg
     3721 cgacggaaat tgaagaggat ggcagcacta tcagtgacga tgcggtgaag caacggggtg
     3781 aaataaaagt gggaataacg ggcaatatca gtcagcgagt ttcgctgcgc ggcagcgtgg
     3841 cgtggcagaa agggagtgat gattttgccc agatggcagg gtttttgtcg atgacagtga
     3901 aatggtaatc gctgtctgac cggcactgtt gccggatgcg gcgtaaacgc cttatctggc
     3961 ctacggggag catttgtagg cctgataaga cgcggcaagc gtcgcatcag gcttggcgcg
     4021 gttgccggat gaatccaaac aacaacctca cttattctgc gtatgcagca tatagttcac
     4081 atccacgccg gggccgagct taaaggtgtt agtgataggg ttgtaatgca gcccggtcat
     4141 atgccgttct ttaagactgg tctgatccac ccagcccagc aattctgccg gtttaataaa
     4201 cttcttcaca tcatgtgtac ctttaggcac catgcgcaaa atatattcag caccaaccac
     4261 cgccatcagc catgacttgc cgttgcggtt aagcgtcgag aaaaagacat cgccgcccgg
     4321 tttcaccagt tgcgcacagg ctctgaccac tgactgagga tcggggacgt gctccagcat
     4381 ctccatacag gtcaccacgt catactgtcc ggcatgtttt gccgcgtgct cttccacggt
     4441 ttcctgcacg taatccacct gaatgccgct ttccagtgcg tgcagttttg ccacctgcaa
     4501 tggctcaaag cccatatcca gaccggtcac cgtcgcgccc tcccgcgcca tactctcggc
     4561 cagaatgccg ccgccacaac cgacatcgag cacctttttg ccaaataaac cgccagcacg
     4621 ctcggcaata tagcccagac gcagcgggtt aatgcggtgc agcggtttga actcaccttc
     4681 aagatcccac cagcgggagg cgacggcttc aaatttagcg atctcttcgt ggtctacgtt
     4741 gtggtttacc ggcgattttt cggcattcat tggcacttct actccgtaat tggcaagaca
     4801 aacgagtata tcaggcattg gatgtgaata aagcgtatag gtttacctca aactgcgcgg
     4861 ctgtgttata atttgcgacc tttgaatccg ggatacagta gagggatagc ggttagatga
     4921 gcgaccttgc gagagaaatt acaccggtca acattgagga agagctgaag agctcctatc
     4981 tggattatgc gatgtcggtc attgttggcc gtgcgctgcc agatgtccga gatggcctga
     5041 agccggtaca ccgtcgcgta ctttacgcca tgaacgtact aggcaatgac tggaacaaag
     5101 cctataaaaa atctgcccgt gtcgttggtg acgtaatcgg taaataccat ccccatggtg
     5161 acttggcggt ttatgacacg atcgtccgta tggcgcagcc attctcgctg cgttacatgc
     5221 tggtagacgg tcagggtaac ttcggttcca tcgacggcga ctctgcggcg gcaatgcgtt
     5281 atacggaaat ccgtctggcg aaaattgccc atgaactgat ggccgatctc gaaaaagaga
     5341 cggtcgattt cgttgataac tatgacggca cggaaaaaat tccggacgtc atgccaacca
     5401 aaattcctaa cctgctggtg aacggttctt ccggtatcgc cgtaggtatg gcaaccaaca
     5461 ttccgccgca caacctgacg gaagtcatca acggttgtct ggcgtatatc gatgatgaag
     5521 acatcagcat tgaagggctg atggaacaca tcccggggcc ggacttcccg acggcggcaa
     5581 tcattaacgg tcgtcgcggt attgaagaag cttaccgtac cggtcgcggc aaggtatata
     5641 tccgtgcccg tgccgaagta gaagttgacg ccaaaaccgg acgtgaaacc attatcgtcc
     5701 acgaaattcc gtatcaggtg aacaaagcgc gcctgatcga aaaaattgcg gaactggtaa
     5761 aagaaaaacg cgtggaaggc atcagcgcgc tgcgtgacga gtctgacaaa gacggtatgc
     5821 gcatcgtgat tgaagtcaaa cgcgatgcgg tcggtgaagt ggtgctcaac aacctctact
     5881 cccagaccca gttgcaggtt tctttcggta ttaacatggt ggcattgcac catggtcagc
     5941 cgaagatcat gaacctgaaa gacatcatcg cggcgtttgt tcgtcaccgc cgtgaagtgg
     6001 tgacccgtcg taccattttc gaactgcgta aagctcgcga tcgtgctcat attcttgaag
     6061 cattagccgt ggcgctggcg aacatcgacc cgatcatcga actgatccgt catgcgccga
     6121 cgcctgcaga agcgaaaact gcgctggttg ctaatccgtg gcagctgggc aacgttgctg
     6181 cgatgctgga acgtgcgggc gatgatgctg cgcgtccgga atggctggag ccagagttcg
     6241 gcgtgcgtga tggtctgtac tacctgaccg aacagcaagc tcaggcgatt ctggatctgc
     6301 gtttgcagaa actgaccggc cttgagcacg aaaaactgct cgacgaatac aaagagctgc
     6361 tggatcagat tgcggagctg ttgcgtattc tgggtagcgc cgatcgtctg atggaagtga
     6421 tccgtgaaga gctggagctg gttcgtgaac agttcggtga caaacgtcgt accgaaatca
     6481 ccgctaacag cgccgacatc aacctggaag atctgatcac acaggaagat gtggtggtca
     6541 cgctttctca ccagggttac gtgaagtatc agccgctttc tgaatacgaa gcgcagcgtc
     6601 gtggcgggaa aggtaaatct gccgcacgta ttaaagaaga agactttatt gaccgactgc
     6661 tggtggcgaa cacccacgac catattctgt gcttctccag ccgtggccgc gtctattcga
     6721 tgaaggtcta ccagttgcca gaagctactc gtggcgcacg tggtcgtccg atcgtcaacc
     6781 tgctgccgct ggaacaggac gaacgtatca ccgcgatcct gccggtgacc gagtttgaag
     6841 aaggcgtgaa agtcttcatg gcgaccgcta acggtactgt gaagaaaacc gtcctcaccg
     6901 agttcaaccg tctgcgtacc gccggtaaag tggcgatcaa actggttgac ggcgatgagc
     6961 tgatcggcgt tgacctgacc agcggcgaag acgaagtaat gctgttctcc gctgaaggta
     7021 aagtggtgcg ctttaaagag tcttctgtcc gtgcgatggg ctgcaacacc accggtgttc
     7081 gtggtattcg tttaggcgaa ggcgataaag tcgtctctct gatcgtgcct cgtggcgatg
     7141 gcgcaatcct caccgcaacg cagaacggtt atggtaaacg taccgcagtg gcggaatacc
     7201 caaccaagtc gcgtgcgacg aaaggggtta tctccatcaa ggttaccgaa cgtaacggtt
     7261 tagtggttgg cgcggtgcag gtagatgatt gcgaccagat catgatgatc accgacgccg
     7321 gtacgctggt acgtactcgc gtgtcggaaa tcagcatcgt aggccgtaac acccagggcg
     7381 taatccttat ccgtacttcg gaagatgaaa acgtggtggg tctgcaacgt gttgctgaac
     7441 cggttgacga ggaagatctg gataccatcg acggcagtgc cgcggaaggg gacgatgaaa
     7501 tcgctccgga agtggacgtt gacgacgagc cagaagaaga ataatttcat cgcttcatgc
     7561 caaaagggag ccatctccct tttttgaatt gaaaagtcca ggctgtaaag tctgggcttt
     7621 tgtcgtatta gggcgcggta aagtttggct gtgcccgtaa aaaatggctg gctatacaca
     7681 aggaatgtgg caatgagtgg tgaaaaaaag gcgaaaggct ggcggtttta tggccttgtt
     7741 ggttttgggg caatagcgct gctatccgct ggcgtatggg cattacaata ctctggcagt
     7801 gggccagaaa aaacgttgtc gccactggtg gtgcacaaca atctgcaaat cgatctcaat
     7861 gagccggacc tctttctcga cagcgactct ctgagccagc ttcccaaaga tctccttacc
     7921 attccgtttc tccacgatgt tctgagcgaa gatttcgttt tctattatca gaatcatgcc
     7981 gatcgtctgg gcattgaagg cagcattcgc cgcattgtct atgaacacga tctcacgctg
     8041 aaagataagc tcttttcgtc actcttagac cagcccgcgc aggcggcact gtggcacgat
     8101 aaacaaggcc atctttctca ttatatggtg ctgatccagc gaagtggttt aagcaagctg
     8161 ctggagccat tgctgtttgc cgctaccagc gacagccagt taagtaaaac ggaaatcagt
     8221 agcatcaaga taaatagtga aactattcct gtttatcagt tgcgctataa cggcaataac
     8281 gtcctgatgt ttgcgactta tcaggacaaa atattggctt tttccagttc ggatatgctg
     8341 tttaaagatg accaacagga taccgaagcc acggcgatcg caagtgattt gttgagtggt
     8401 aaaaaacgct gggaaacccg ctttggcctg gaagcgcgta ctgccgaaaa aacgccagta
     8461 cgccagcgca tcgtagtcag cgccaggttg ctggggtttg gctaccagcg attaatgcct
     8521 tcttttgctg gcgtacgctt cgaaatgggt aacgacggct ggcacagttt tctggcgtta
     8581 aacgatgaat ccgccagcgt agatgccagt ttcgatttta cgccggtatg gaacagtatg
     8641 cctgccggag ccagcttctg tgtggcggtg ccgtattcac acggtattgc cgaagagatg
     8701 ctttcgcaca tcagccagga aaacgacaag ctgaatgggg cgttagacgg tggcgcgggg
     8761 ctgtgctggt atgaagactc aaaattgcaa accccactgt ttgttggtca gtttgatggc
     8821 actgccgaac aggcgcaatt gccagggaaa ctgtttacgc aaaatattgg tgcgcacgaa
     8881 agcaaagcgc cagaaggtgt tttgccggta agccagactc agcagggcga agcgcaaatc
     8941 tggcgtcgcg aagtgagttc ccgatacggt cagtatccga aagcgcaggc ggcgcaaccc
     9001 gatcaattaa tgtcggatta ttttttccgc gtgtcgctgg cgatgcaaaa caaaacgctg
     9061 cttttctccc tcgatgacac gctggttaat aacgccctgc aaacactgaa taaaacccgc
     9121 ccggcaatgg tggatgtaat acccactgat ggcatcgttc cgctctatat caatccacaa
     9181 ggaatggcga aactgctgcg taacgaaacg ctgaccagtc tgccgaagaa tctcgaaccg
     9241 gttttttata acgccgcaca aactttatta atgccgaagc tggacgcttt atctcaacaa
     9301 ccgcgttatg tcatgaagct ggcccagatg gaacccggtg tcgcctggca gtggctaccc
     9361 ataacctggc aaccgctatg agacacgggc tgctggcgct gatttgctgg ctgtattgtg
     9421 ttgttgccca tagcgaaatg ctaaatgtcg aacaatccgg gctgtttcgc gcgtggtttg
     9481 tgcgcattgc acaggaacag ctccgccagg ggccaagccc acgctggtat cagcaggatt
     9541 gtgcgggcct ggtgcgattt gcggtgaacg aggcgctgaa agttcacgac agtaaatggc
     9601 taaaaagtaa cggtttatct aaccagtatt tgccgccaga gatgacgcta acacctgaac
     9661 agcgtcaact ggcgcaaaac tggaatcagg ggaatgggaa aaccggcccc tatgtgaccg
     9721 cgattaattt gattcagtac aacagccagt ttattggcca ggacataaac caggcactgc
     9781 ctggcgatat gatttttttc gatcagggcg atgcccagca cttaatggtc tggatggggc
     9841 gttacatcat ctaccacacc ggaagcgcca cgaaaactga caacggaatg cgcgcagtca
     9901 gtctgcaaca acttatgaca tggaaggaca cccgatggat acccaacgat tccaatccca
     9961 atttcattgg catttatcgt ttaaattttc tggcgcgata gcagcgtgct tgtccctgtc
    10021 tcttgtggga acagggcttg ctaatgctga ggattcgctt ccttccagta actatgtgcc
    10081 gcccgccggg ggaacattct ttttgctcgc tgacagcagt tttagcagca gtgaagaggc
    10141 gaaagtgcga ctggaagcgc cggggcgtga ttatcggcgc tatcagatgg aagagtacgg
    10201 cggcgtggac gttcgcctgt atcgtattcc tgacccgatg gcatttttgc gccagcagaa
    10261 aaacctgcat cgcattgtgg tgcaaccgca atatctgggc gacggactga acaatacgct
    10321 gacctggctg tgggataact ggtacggcaa atctcgccgc gtgatgcagc gtactttctc
    10381 ttctcagtca cggcagaatg tgactcaggc gttacccgaa ttacagctcg gtaatgccat
    10441 tgttaaacct tcccgttatg tacagaacag ccagttttca ccgctgaaaa aatatccact
    10501 tgtggaacag ttccgctatc cactatggca ggcaaaaccg gtcgagccgc agcaaggggt
    10561 aaaactggaa ggcgcatcca gcaatttcat ctcgccgcag ccgggcaaca tctatattcc
    10621 tctcggcaaa caagagccgg gactgtatct cgtcgaagcg atggtgggtg ggtatcgggc
    10681 gacgacggtg gtgtttgttt ccgataccgt ggcgcttagc aaagtgtcag gtaacgagct
    10741 tctggtgtgg accgcgggga aaaaacaggg tgaagcgaag cccggctcag agatcttgtg
    10801 gactgacggt cttggcgtga tgacccgcgg tgtgaccgat ggcagcggta ccttgcagtt
    10861 acaacatata tcgccagaac gttcatacat tctgggtaag gatgctgaag gcggcgtttt
    10921 tgtctccgag aactttttct acgaaagcga aatctacaac acccgcttgt atatctttac
    10981 cgatcgcccg ctatatcgcg caggcgatcg tgtcgatgtt aaagtgatgg gtcgcgagtt
    11041 ccacgatccg ttgcattcat cccccatcgt cagcgcaccg gcgaagcttt cggtgctgga
    11101 cgccaacggc agtctgttgc aaaccgtcaa tgtcacgctg gatgcgcgta atggcgggca
    11161 ggggagtttc cgcctgccag aaaatgccgt cgccggaggt tatgagttac gtcttgctta
    11221 ccgcaaacag gtctatagca gcagttttcg cgtggcaaac tacatcaaac cccattttga
    11281 gattggttta gctctcgaca aaaaagagtt caaaactggc gaagcggtca gcggcaaact
    11341 gcaactgctt tacccggatg gcgagccggt aaaagatgcc cgcgtgcagt taagtttgcg
    11401 cgctcagcaa ttatcaatgg tcggtaacga tttgcgttat gccggacgtt tcccggtgtc
    11461 gatggaaggc agtgaaacgg tttccgacga taatggtcat gtggcgttga atctccccgc
    11521 cgccgataaa ccgagccgtt atttgttaac cgtctccgcc agtgacggcg cggcgtatcg
    11581 agttaccacc accaaagaga tcctcattga acgtggcctg gcgcattact ctttaagtac
    11641 cgccgcacaa tacagtaata gcggcgagtc ggttgtgttc cgttatgccg cgctggaatc
    11701 ttcaaaacag gttcctgtta cgtatgaatg gttgcgtctc gaagaccgca cgagtcacag
    11761 cggagagcta cagtcaggcg gcaaatcctt taccgtcaat ttcgctacac ctggcaacta
    11821 caatctgaca ttacgcgata aagacggctt aattctcgcc gggttaagcc atgcagtcag
    11881 cggtaaggac agcacgtcgc atagtggtac ggtagatatc gtggcagata aaacactgta
    11941 tcagcctggc gaaaccgcga agatgctgat taccttcccg gagccaattg acgaagcatt
    12001 attgacgctg gaacgcgatc gcgtagaaca gcagtcgctg ctatcgcatc cggcaaactg
    12061 gctaacgcta caacgtttaa acgataccca gtatgaagcc cgcgtaccgg tgagcaattc
    12121 ctttgcgcct aacatcactt tttcggtgct gtatacccgt aacggtcagt acagttttca
    12181 gaacgccggg attaaagttg ccgttcccca gctggatatc cgggtgaaaa cggacaaaac
    12241 ccattaccag cctggtgaac tggtcaatgt cgaattaacc tcgtcgctga aaggtaaacc
    12301 tgtttctgcg cagttaacgg taggcgtggt cgatgaaatg atctacgcgc tgcaaccgga
    12361 aatcgcgccg aatatcggca aatttttcta tccgctgggg cgtaacaatg tgcgtaccag
    12421 ctccagtctg tcgtttatca gctacgatca ggcgctctcc agcgagccgg ttgcgcctgg
    12481 cgcgactaac cgcagcgagc ggcgagtaaa aatgctggaa cgtccacggc gtgaagaggt
    12541 ggataccgcg gcctggatgc cgtcactcac aaccgataaa caaggcaaag cgtatttcac
    12601 gttcctgatg cctgattcgt taacccgctg gcgcatcacc gcgcgtggga tgaacggcga
    12661 cgggctggtc gggcaggggc gtgcttattt gcgttcggaa aaaaatctct acatgaagtg
    12721 gagtatgcca acggtatatc gcatgggtga caaaccggcg gcaggactgt ttatcttcag
    12781 tcagcaggat aacgacccgg tggcgctggt gactaaattt gcaggtgctg agatgcgcca
    12841 gacgctgacg ctgcacaaag gggcgaatta tatttcgctg acgcagaata ttcagcaatc
    12901 tggcttgtta agtgcagaac tgcaacaaaa tgggcaagta caggacagca ttagcacaaa
    12961 actgtctttt gtggataaca ggtggcccgt tgaacaacag aaaaatgtca tgctcggcgg
    13021 tggcgataat gcgctgatgt tgcccgagca ggcgagcaat atccggctac aaagtagcga
    13081 aacgccgcag gagattttcc gtaacaatct tgatgcgtta gtcgatgaac cgtggggtgg
    13141 cgtgatcaac accggtagcc gtctgatccc gctcagtctc gcctggcgtt cgcttgccga
    13201 tcatcaaagt gccgccgcta acgacattcg tcagatgatt caggataacc gtctgcggct
    13261 gatgcaactg gcggggcccg gagcgcgctt tacctggtgg ggtgaagatg gcaatggcga
    13321 cgccttcctc acggcatggg catggtacgc cgactggcag gccagccagg cgctcggcgt
    13381 aacgcagcaa ccggaatact ggcaacatat gctcgacagt tatgccgagc aggcggacaa
    13441 catgccgtta ttgcatcggg cgctggtgct ggcgtgggca caggagatga atctgccgtg
    13501 caaaacgttg ttgaaagggt tggatgaagc tatcgcccgg cgcggaacta aaactgaaga
    13561 tttctctgag gaagacacca gcgatatcaa tgacagcatg atcctcgata cacctgaatc
    13621 tccactggca gatgcggtgg caaacgtctt aaccatgacg ttgctgaaaa aagcgcagtt
    13681 gaagtccact gtgatgccac aggttcagca atatgcgtgg gataaagcag caaacagcaa
    13741 ccaaccgctg gcgcacacgg ttgtgctgct caatagtgga ggcgacgcta cccaggcggc
    13801 cgctatttta agtggtttaa ccgctgagca atccaccatt gagcgcgcgt tggccatgaa
    13861 ctggctggcg aaatatatgg cgacgatgcc tccggttgtg ttacctgcgc ctgcgggcgc
    13921 atgggccaaa cataagttaa ccggaggagg cgaatactgg cgttgggttg gtcagggcgt
    13981 gccggacatt ctctcttttg gtgacgaatt accgccgcaa aacgtgcagg tccgctggcg
    14041 tgaaccggca aaaacggctc aacaaagtaa tattccggtg accgttgaac gccagttgta
    14101 tcggcttatc cccggtgaag aagagatgag ctttacctta cagcctgtca ccagcaatga
    14161 gattgacagc gatgcgctgt atctcgatga aatcacgctt accagcgagc aggatgccgt
    14221 tctgcgctat ggtcaggtgg aagtaccgct gccgccgggg gctgacgttg agcgtacaac
    14281 ctggggcatt tcggtcaata aaccgaacgc cggaaaacag caaggacaac tgctggaaaa
    14341 agcgcgcaat gaaatgggtg aactggccta tatggtgccg gtaaaagaac tgacgggaac
    14401 ggtcaccttc cgccatttgc tgcgtttctc gcaaaaaggg caattcgttc tgccgcctgc
    14461 ccgttatgtg cgttcctatg cacctgcaca gcaaagtgtt gcgccaggga gcgaatggat
    14521 cgggatgcag gtgaaataag tgaactggta cagaatcgtc tggctattgg cgttggtaac
    14581 tctgccaacg cttgcggagg agacgccgtt acagctggcg ctgcgtggtg cgcagcacga
    14641 tcaactgtat cagctgtcgt catcaggcgt gaccaaagtc tcggtattac ctgacacgct
    14701 caccacgcca ttagggagtt tgtggaaact gtatgtttac gcctggctgg aggataccca
    14761 tcaaccggag cagccatacc agtgtcgcgg caactccccg gaagaggttt attgttgtca
    14821 ggctggggag agcataacgc gcgattccgc gctggtacgt tcctgtggac tctattttgc
    14881 gccgcagcgg ttgcatattg gcgcagatat gtgggggcaa tactggcaac agcgtcaggc
    14941 acctgcgtgg ttagcgtctc ttactacact gaagccagaa acctcggtaa cggttaaatc
    15001 gctgcttgat tcattagcga cgttacctgc gcaaaacaaa gctcaggaag tcttactgga
    15061 tgtggtgctg gacgaggcga aaatcggtgt cgcatccatg ctcggtagtc gggtacgggt
    15121 gaaaacctgg agctggtttg ccgatgataa acaggaaatc cgtcagggtg gttttgctgg
    15181 ctggttaacc gatggtacac cgctgtgggt tactggcagt ggcaccagca aaacggtact
    15241 gacccgttat gcgacggcgc ttaatcgggt tttgccagtg ccgacgcaag ttgccagtgg
    15301 gcaatgtgtg ctggtggatc tgtttgctcg ttatccgctg aagaaagtta ctgaggaaaa
    15361 gagtacgacg gcagtcaaac caggcgtgct caatgggcgc tatcgtgtga cgtttgccaa
    15421 tggtaaccac atgacatttg tcagccacgg tgagacaacg ttactgacgg taaagggcaa
    15481 actgaaatta cagtcgcatc tcgatcgtga agagtacgtt gccagagttc tggatcgcga
    15541 ggcgaaaagt acgcctcctg aggcagcgaa agccatgacc gtggcgattc gcacgttttt
    15601 acagcaaaac gccgatcggg aaggcgattg cctgaccatc ccggacagca gcgctacgca
    15661 gcgtgtttct gcttcgccag ctaccacggg ggcgcgaacg atgaccgcct ggacgcagga
    15721 tctcatctat gcgggcgatc cggttcatta tcatggcagt cgcgctaccg aagggacgct
    15781 ttcctggcgg cacgctactg cccaggcggg gcagggagag cgttatgatc agatactcgc
    15841 gtttgcttat cccgacaaca gccttagccg ctggggagcg ccacgctcaa cctgccagtt
    15901 attacccaaa gcgaaagcct ggctggcgaa aaaaatgccg cagtggcggc gtatattaca
    15961 aggtgagacg ggatacaacg aaccagacgt gtttgcggtc tgtcgtttag tctcaggttt
    16021 cccctatacc gatcggcagc agaaacggct gtttattcgt aatttcttta cgcttcagga
    16081 tcggctggat ttaacccatg agtatctgca cctggccttt gatggttatc ccaccgggct
    16141 tgatgagaac tatatcgaaa cgctgacccg acaattatta atggactaat gctatgcgaa
    16201 agatttttct tccgttgtta ctggtggcgc tttcgcctgt cgctcacagt gaaggtgtgc
    16261 aggaagtcga gattgatgca ccgctctctg gctggcatcc ggcagagggc gaagatgcca
    16321 gtttttcaca aacgatcaat tacccggcgt cgtccgtcaa catggcagat gatcaaaata
    16381 tctcagcgca gatccgcggc aaaataaaga attatgctgc ggcgggtaaa gttcagcagg
    16441 gccggctggt ggtcaacggt gccagtatgc cgcagagaat tgaatcagat ggttcttttg
    16501 cacgccctta tattttcact gaaggcagca acagcgtgca ggtcatcagc ccagatgggc
    16561 aaagccgaca aaaaatgcag ttttactcaa cgccgggtgc tggggcgatt cgcgcgcgtt
    16621 tacggctggt tctctcctgg gatacggaca ataccgacct cgatcttcat gtcgttacgc
    16681 ctgacggtga acatgcctgg tacggtaaca cagtgctgaa aaacagtggc gcactggata
    16741 tggatgtcac gacagggtac ggacccgaga ttttcgccat gccagcgcca gttcacggcc
    16801 gttatcaggt gtatatcaac tacttcggcg ggcgcagtga aacggaatta actacggccc
    16861 aactgacgct gatcaccgat gaagggtcgg tcaatgagaa acaggaaact tttattgtac
    16921 cgatgcgtaa tgctggcgag ctgacgctgg tgaaaagttt tgactggtga ttaatggggt
    16981 aaccttcgcc gtcgatgttc aagaaaacgc ccgataactt tcgctatcgg gcgtttttat
    17041 tggttaattc aaccgctcaa tcaccatcgc aattccctgg ccgccaccaa tgcatagtgt
    17101 tgccagcccc agcgttttat cgcgtgcctg cattgcatgt aatagtgtga ccagaatacg
    17161 agcaccactg gcaccgatag gatgtccgag cgcgatggcc ccgccgttga cattcacttt
    17221 ctcaggatca aagcccaggg ttttcccaac ggcaaggaac tgtgcagcaa atgcttcatt
    17281 agcctcaatg agatcaatat ccgccagttg cagccccgcc agttgtaacg ctttttgcgt
    17341 agcaggtact ggccccatac ccatcaatgc ggggggcacg ccaccgctgg cataactttt
    17401 aatgcgtgcc aggggattca ggcctgctgc cagcgccgca gattcttcca taatcaccag
    17461 agcggcagca ccgtcgttaa tacctgacgc gttcccggcg gtgactgttc ctgctttatc
    17521 gaaggccggg cgcaatgcgc ctaacgcttc agccgtagaa tccgctttcg ggaattcgtc
    17581 ttgactgaag acgaaggttt tcttccgggt gacaacattt accgggacga tttcggctgt
    17641 aaaagcaccg gactcaattg cggctgccgc tttacgctgt gaatgtagcg ccagttcatc
    17701 ctgcatttca cgggtaattc cgtactcttt agccacgttt tcggcggtaa tccccatatg
    17761 ataaccatgg gtggcgcaca tcaggccatc gcgcaggatt acgtcataaa cctgtccgtc
    17821 tccaagacga taaccagagc gtgcttttgc atcgagtaag tagggcgcta aactcatatt
    17881 ttccataccc cccgccacaa tgctctgtgc ctgacctgcc tgaatcgcct gtgcagcaag
    17941 cgccacgctt ttcagacctg aaccgcacac tttgttgacc gtgaatccgc acaccgtttc
    18001 agctagtccg ctttttagca gcgcctgacg tgccggattt tgccccagtc cggcttgcaa
    18061 cacgttaccc ataatcactt catcaacgtg tagtgaatcg atttttgcac gttcaatggc
    18121 ggctttaatt actgtcgccc ccaggtcgat ggcgctggtg gaagcgagtg aaccgttaaa
    18181 actaccgata gcagtacgta ccgcactgac gatgacacaa tttttcattt tatattcctt
    18241 ctgtctgtag ggttgccgtc agaacagcgt taaaccaatg acgaaaatga cgccggagaa
    18301 gagcagggca gtgatgcaat agcccatgat gtcgcggaca ccgagtccgg cgattgccag
    18361 tgctggcagc gcccagaacg gttgtgccat gttcatccat tgctcgccgt aggcgatcgc
    18421 cattaccgat ttaccgagat cagcgcccag cgcctgggct gcgggcatca cgaacggccc
    18481 ctgaataacc cagtgcccgc cgccagacgg aacggcgaag ttaatcagtg cagaactaaa
    18541 aaaggtcatg accgggaagg tgtctttgtt cgcaacattg atgaagaatt cggtaatgag
    18601 tccgcccaga ccggagtgct ccatcatcag ttggatcccc gcgtagaagg ggaattgcac
    18661 cagaataccg gcggtactgc gtgctgccgc gctgatagca cgcatataag ccattggcgt
    18721 tttatgtagc agcagacccg caatcataaa catcaggttg acggtattga tggtgatgtt
    18781 gaagccatgt tcgctgaagt acatcgccag gtaagcgata ccgagtgcgc cgatgatcaa
    18841 cgtcagaatg cggctttctt ccagtcgctc ggacggtgga gcatctttcg gtagttgctt
    18901 ttgaaaatca gcctcttcca tgagtagttt cggatcgata ctcaccacgt cagacggttt
    18961 tggcatcatc atgcgggtga taaatggcat caccacaatc aacgccacag tgatgaaaat
    19021 attaaaacca ctgaacagag tatcgcccac cgggatcagc ccagcgatat gctcaaccgg
    19081 gttgcccggt gttgcagcca acagaggcat tgagccagag aagccgccac cccaggtgag
    19141 aaaaccaatg taggcgcagg caatgagcaa cggataatca gaaccgggta ctcgccgggc
    19201 gacttcacgg gcgaacattg caccgacaac caaaccaaat ccccagttga tgacacaagc
    19261 gactgaaccg aagaaagtaa ccagcatgac gccctgtacg ggcgtctttg cggcggaggc
    19321 ggcagtacgc agcaaacttt tcaccggagc ggagctggca agggcatgac cggtaacgat
    19381 gataagcgcc atctgcatac caaacgccag caagttccag aaaccgtcac cccacatttt
    19441 caccatgctg atcggcgttt gtggtgttaa ccaaagcgcg atcacgaatg ttagcaatgt
    19501 cagcaacatg gcaaagatca gtggatcggg aagccaccgg ctgacaaaac gcgtcataaa
    19561 acgcgatatg cgaccaatca taaatcaccc cgttgcgtat tcagatcggc agctacttca
    19621 aaccgagctt ctgttttggc acgcacggtg gctaaatcac acccgtcggc aatttcggtg
    19681 agccacattt tgccgtcaat aaaacgaaag acagccagtt cagtaaccag catatgcacc
    19741 gcatgttgcg cagtgagtgg catggtgcag cggcgcaaaa tttttgctga accatctttg
    19801 gcgcaatgtt ccatggcgat gatcactttg cgtgacccgg tcaccagatc catcgcgcca
    19861 cccataccgg gcaccatttt cccaggcact acccagttcg cgaggtttgc ttcttcgtct
    19921 acttgcaaac cgccgagcac gcaggcatca atatgaccgc cacggattag cgcaaatgac
    19981 atggcgctat caaacatggc tgcaccgggt aaaatgccgc acggttgtcc gccagcattc
    20041 accagatctg gatgcgctgt tgtgaccggg cctaaaccga ggaagccgtt ttccgattgc
    20101 agagtgatat gaataccctc cggtaaataa ttggcgacca ttgtgggtaa accgatccct
    20161 aagttaacga tgtcaccatc acgaagctct tgcgccacac ggcgcgcaat acgttgtttc
    20221 gcatccatta tttgctctcc tgtgaaacga tgatgtggtc gataacggca ccaggggtga
    20281 caatatggtc aggttgcagc tcgccggttt cgaccagttc atccggctct accagcgtga
    20341 tatcagccgc aagggctatc agggggttaa agttgcgggc gctaaggtga taggtcaggt
    20401 tgccaagtgt gtcgcaacga tgagcgcgaa ttagcgccag gtcggcgcgc agcgggcgtt
    20461 cgagcagcca ggttttaccg tcgagtgtca gtgtctgttt gccttcctct acgacggtgc
    20521 cgacaccagt tggggtgaga aaaccaccaa gtccagctcc gccacagcga atttgctcga
    20581 ttagcgtacc ttgcggcacc agaacgacgt ccatctcacc agatatcatg cgccgacctg
    20641 tttccgggtt ggtgccgata tgtgaagcaa tcactttgcg gactcgacca ttgacgatga
    20701 gcggaccgat gccggtatca acaaacgcgg tatcattggc tatcaatgtc aggtcgcgaa
    20761 caccagattc cagtaatgct tcaaccaggc gggatggagt gccaatcccc ataaatccgc
    20821 ccaccatgat ggtcatgccg tcacgaaaga agccggtggc gtcttgtaat gtcatcaatt
    20881 ttgttttcat tttttatccc tcttgcatac accgttgtgg gtacagtcag ggaatagcaa
    20941 ggggagtgcc agaatgagat tattgaatgt tgttatttaa ctaattgaat agaaaaggat
    21001 aaattagatg ggggttcata aggagaatga gcggctatgc agaaaattgc gcactgtgca
    21061 aatttctgca tagcaagttt tggcattata catccgccgg atcgataccg tattcctgga
    21121 gtttatacat caatgcacgg cgactgatgc ccagcattaa agcagtgcgg gttcggtttc
    21181 cttcttgttg ttccagcact tccataatga tgcgtttttc gacgcgttta atttcctctt
    21241 ttaaattacg ctcaccgaca ggggctgttt ttgcttcacc agcattacag actggctgac
    21301 gaatctgcgg cggaagatcc tcagaaaaaa tgatcgggcc tgaattcatc acgacggcgc
    21361 gttcaataac gttggaaagc tctcgaatat ttcccggcca tgaccaggcg gtaagcagtg
    21421 acattgccat cggatcgatg tcgataatat cgcgctgatt ctcactactg aatttttgca
    21481 aaaagtgatt agctaacagg gaaatatctt cccggcgatc gcgcagaggc ggcagtatta
    21541 aatgaataac gttaaggcga taaaagagat cttcacggaa ggtgccttct ttcaccattg
    21601 cctgcaagtc gcggttggtg gcagcaatga cgcggatatc aacttttatg gtctgatggc
    21661 cgccaatccg ttcaaattcc cgttcctgca gaatgcgtag taatttggct tgcagtacca
    21721 gcggcatttc gccaatttcg tcgaggagca gagtaccttc gttggcacgt tcaaataatc
    21781 cctgacgcaa ggtttgtgca ccagtaaatg cacctttttc atgaccaaac agttcacttt
    21841 cgagcaacga ttccggcagt gccgcgcagt tgactttaat gaacggcccc tttgcccgcc
    21901 gcgaattgta gtgaatcgct ctggcaatca actctttccc ggtgccgctt tcaccgctaa
    21961 tcaagacgct ggcctgagaa agggcaattt tggcggtgtc tttgcagatg tccatcatcg
    22021 ccgggctgtt ggtgagaatg tgcccccatt gccagctggt gctcagtgcc tggtgcagat
    22081 gacggatctc ttttttcatt gactggagtt gtaaagcgcg ctgaacgatt aaattcaact
    22141 catcgagatc aaacggtttg ataacatagt cgaaggctcc gcagcgtagt gcttcgacgg
    22201 cggtttccac ttcagcatag gccgtcatca gaataacggg tgtccgggtc tcatggctgc
    22261 gcatctcctt tagtgccttg atgccgtcca tctctggcat gcggatatcc atcaacacca
    22321 cgtcagggtg aatatcggca aacaggtgta atgctgtgcg cccgttgttc gcacaatgtg
    22381 tttcgaatcc ttgtagtgca aaagcggtgc tcagcatacg gcgaacatta tcttcatcat
    22441 ccacaataag gatgcgatta atagcagtca tacagtctga tttccctgcg ggttgatcgg
    22501 taaaataagc gtgaaggttg cgccgtagcc cggcaaactg gcgacgcgaa tatcaccctg
    22561 atgggcatta atgatgcgtt gacttaacgc cagaccaagc ccggttcctg aggctttggt
    22621 agtgaaaaag ggatcgaaga tctttttttg cagcgagaga tcaatgccac tgccgttgtc
    22681 ctctatcgaa atggcctgtt gcgagtcgct atactgccag gttcgaatgc gaattttccc
    22741 tcgtgcactg atagcctgga cggcattgat caggatattc agtagtacct gtttgagcag
    22801 ttcacgatcg gcgttaatcg ggcttaattc attatccagt tcgcttatga agtcgacccg
    22861 cgcttgtacg ccggcggttt gtaccagtac cagagtttct tcaaccaatg cattgaggct
    22921 gacttgttgc cattgactgt ggcgtgggcg tgaaaattcg agcaattgct gaataacttt
    22981 gttaattgaa tcgatttctt tgagtactac ggacagatat tcctgatgta ttgggtcacg
    23041 ggtttgttgg cgcaagatct gtacataacc acgaatagcc gttagcggat tacgtacttc
    23101 atgcgccacg ccagccatca gctcacccag tgtggcgagg cgttctgctt gcgccatgcg
    23161 gcgctgggtt tctttgcggg cagttaaatc agagaaaatc accaaagcac ctatcatttc
    23221 accgtgcgtg ttatgaatac gactggtcgt gacactgagt tcaatggtgc ggtcacggcc
    23281 tggaaaactg atctccagtg caacatgttc ggtgccatgt tccagcgtat ccagtacggg
    23341 actgtagaac tgagtattgt cgaacaacat ggagtaaggc tgccctacca gttcatgacg
    23401 ttgatagcca gtgatgactt ctgctgctgg gttcatggtg gttacatcac cctggcggtc
    23461 aatggcaatg acgccatcgg cagcgttttc aataatcaga tcgttaagtg ttcgcgtttc
    23521 acgcagtgcc tgggcgaggt tgttaacact ctgactgatt tgccccattt caccgggcaa
    23581 ttgtggtaat cgagtgggaa tattttgagc cagagtcgag aggccatcgg tgatgatatc
    23641 tatattggcg ctcaggcgac gggagaaaag gacaatcaac agcaggctta tcagcaaacc
    23701 ggcggtgagc acaatgataa tcctcacatc cattttccag gcctggcggc gaatatcttc
    23761 ggttaattca ttggcccaga tatagccgag gatttcacca ttacgctcaa tgggaatcat
    23821 tgaattcaaa atatcgccgc gcacctgcct gcctgaataa accaaagggg tatttgtacg
    23881 catgacttcg cgaccagggt gatctgcggc aatggtaacg cccacattat tctgatatag
    23941 cgctgaaggc gcgtaggtga ttatcgcatc cagcgttttg ttgtaataac cagcgccgat
    24001 gccagggaag gcgtgagtga tattttcggt aatgggggca agttctgcat ttaatgcgcg
    24061 gatacgctcc tcacgcggta agtcgatgta gagatcgtag cgattgccta gcgcctgatt
    24121 aagcaggttg accacggcag aaagtttttt ctctttttca gataagactg ctgaacgtcc
    24181 ttccgtttct acgatataac caatagtaag cgttgggaca atgaccatca ggattgccat
    24241 caggatcatt tgattgcgta agcggcgtgg ataaatccac ttcatataat gcatgctgaa
    24301 atccactagt cttgtccggt atatgacgat tatcagaggt taaggtgatg atttctcggc
    24361 ggtgtatcat attccagaga agagagaaca ttgcggtaac acgcttttac cgctacctta
    24421 accacactcc atcggtcacc tgaggcggag cttcgcccct ttgaaatacc ttgcttcttt
    24481 tcgtacaacc ctgaaagcct cgcgctacat gttcagagca ttggcgttag tgctctggct
    24541 gttgattgct ttttcatccg ttttttacat cgttaatgcg ttacatcagc gagaatcgga
    24601 aattcgtcag gaatttaatc tgagttccga tcaggctcag cgctttattc aacgcacctc
    24661 tgatgtgatg aaagagctga agtacatagc tgaaaatcgc ttatcggcag aaaacggtgt
    24721 gctttccccg cgtggacgag aaacgcagac ggatgtgcct gcgtttgaac cgctgtttgc
    24781 tgactctgat tgttccgcaa tgagtaacac ctggcgaggt tctctggagt cattggcgtg
    24841 gtttatgcgc tactggcgcg ataatttttc tgcggcttac gatctaaacc gggtattttt
    24901 aatcggcagc gataacctct gcatggccaa tttcggtctg cgtgatatgc cagtggaacg
    24961 cgataccgcg ctaaaagctt tgcatgaacg cattaataaa tatcgaaatg caccacaaga
    25021 tgatagtggc agtaacctct actggatcag cgaaggtccg cgccctggcg tcgggtattt
    25081 ttacgccttg acgccagttt atctggcgaa ccggttgcag gcgcttttgg gtgtcgagca
    25141 aaccatccgg atggagaact ttttcttacc tggtacgttg ccgatggggg ttaccattct
    25201 tgatgaaaat ggtcataccc ttatttcgct taccggacca gaaagcaaaa ttaagggcga
    25261 tcctcgctgg atgcaggaac gctcctggtt tggctatacg gaagggttcc gggaactggt
    25321 gctgaagaaa aatctgccac cctcatcgct aagcatcgtt tattcagtgc cagttgataa
    25381 ggtgctggaa cgcattcgca tgttgatcct taacgcaatt ttgctgaatg tgcttgccgg
    25441 agctgcattg tttactctcg cgcggatgta cgagcggcgt attttcattc cggcggaaag
    25501 cgacgccctg cgactggaag aacatgagca gtttaatcgc aagattgtcg cctccgcgcc
    25561 agtgggtatc tgcattttgc gtaccgctga tggcgtcaat attttaagta acgaactggc
    25621 gcatacttat ctcaatatgc ttacgcatga ggaccgccaa cggctgacac aaattatctg
    25681 tgggcagcag gtcaattttg ttgatgtcct gaccagcaac aataccaatc tgcaaatcag
    25741 cttcgtccat tcgcgctatc gtaatgaaaa cgtggccatt tgtgtgctgg tggatgtttc
    25801 ttcgcgcgtg aagatggaag agtcgttgca ggagatggca caagcagcgg aacaggcgag
    25861 ccagtcaaaa tcgatgttcc tggccaccgt cagtcatgag ctgcgaacgc cgctgtatgg
    25921 cattatcggt aacctggatc tgttgcaaac caaagagtta ccgaaaggcg tcgatcgtct
    25981 ggtgacggca atgaacaact cttccagcct gttgttgaaa attatcagcg atattctcga
    26041 tttctcgaaa attgaatcag aacagttgaa gatcgaaccg cgtgagtttt caccgcgtga
    26101 agtgatgaac cacatcaccg ccaactattt accgctggtg gtacgcaagc agttaggctt
    26161 gtactgcttt attgaaccgg atgtgccagt ggccttaaat ggcgacccga tgcgtttaca
    26221 gcaggtcatc tccaacctgt tgagtaacgc cataaaattc accgataccg gctgtatagt
    26281 tttgcatgtt cgcgctgatg gcgattatct ctctatccgt gttcgcgata ccggcgtggg
    26341 gataccggcg aaagaagtgg tgcgcttgtt tgatcccttc ttccaggtcg gaacgggcgt
    26401 acagcgtaac ttccagggga ctggtctggg tctggcgatt tgtgaaaaac tgatcagcat
    26461 gatggacggc gacatctcgg tagattcaga accgggaatg ggcagccagt ttaccgtgcg
    26521 tattccgtta tacggcgctc agtacccgca gaaaaaaggt gtggaagggt tgagtggtaa
    26581 acgctgctgg ctggcggtcc gcaatgcgtc gctctgtcag ttcctggaaa ccagtttgca
    26641 gcgcagcggc atcgtcgtta caacatacga agggcaggaa ccgactcccg aagatgtgtt
    26701 aattactgac gaggtagtga acaaaaaatg gcagggcaga gcggtagtga ccttctgtcg
    26761 tcgccatatt ggtattccgc tggagaaagc gccaggggag tgggtacata gtgtggctgc
    26821 tccgcatgag ctaccggcat tgttggcgcg tatttatttg atcgagatgg agagcgacga
    26881 tcctgctaac gctctgccgt cgacggacaa agcggtcagc gataatgacg atatgatgat
    26941 tctggtcgtg gatgatcatc cgattaaccg gcgtctgctg gcagatcagt tgggatcgtt
    27001 gggctatcaa tgtaaaaccg cgaatgatgg cgtcgatgcg cttaatgtac ttaacaagaa
    27061 tcatattgat atcgtgctta gcgacgtcaa tatgccaaat atggatggtt accgcttgac
    27121 gcaacgcatt cgtcagttgg gactgacgtt gccggtaatc ggagtaactg ctaatgcgtt
    27181 ggctgaagag aagcagcggt gtctggagtc cggtatggac agctgcctgt cgaagccggt
    27241 aacgctggat gtgataaaac agacgctgac ggtatatgcc gagagggtca ggaaatcgcg
    27301 ggaatcgtag gacggataag gtgtttatgc cgcatctggc attcagtgca aatgccagat
    27361 gcgacgctga cgcgtcttat ctggcctact ttaatgtaat ctgttgaaat aatgggaatc
    27421 gtaggacgga taaggcgttt acgccgcatc cggcaatccg tgcaaatgcc agataagaca
    27481 ctgacgcgtc ttatctggcc tacaggtgat tagtctttat ctgccggact caacgtcaca
    27541 gaagagagat aattcagcag agcaatatcg ttctcgacac ccagcttcat catcgcagat
    27601 ttcttctggc tactgatggt tttaatactg cggttcagct ttttagcgat ctcggtcacc
    27661 aggaagcctt ccgcaaacag gcgcagaact tcactctctt ttggcgagag acgcttgtca
    27721 ccgtaaccac cagcactgat tttttccaac aggcgagaaa cgctttccgg ggtaaatttc
    27781 ttccctttct gcagcgcggc gagagccttc ggcaaatcgg tcggtgcacc ttgtttcagc
    27841 acgatccctt cgatatccag atccaatacc gcactaagaa tcgccgggtt gttgttcatg
    27901 gtcagaacaa tgatcgacag gcttgggaaa tggcgcttga tgtacttgat taaggtaatg
    27961 ccatcaccgt acttatcgcc aggcatggag agatcggtaa tcaacacatg cgcatccagt
    28021 ttcggcagat tgttgatcag tgctgtagag tcttcaaatt cgccgacaac attcacccac
    28081 tcaatttgct caagtgattt gcgaataccg aacaagacta tcggatggtc atcggcaata
    28141 attacgttca tattgttcat gtaataggct accttgctac agcaagctct tgacataact
    28201 gtcaatgtcg ctgatgtatt tttctattcc tggaacatcc ttctcacgaa tcagatgttc
    28261 cagcgtttca cataactgct tgccgggtac cagatttagc atggcaaata cgcctttaag
    28321 acgatgagcc gtttgggcta acgcagcaaa gtcgctggtt gctgcttcag tatatagcct
    28381 cttaacatca tccggtactg tgtctacaaa gagcgcataa tagccgctgg catggagttg
    28441 cgcattttca tctccgccca gaggcgattc tgtcacctct tcctgcgcca gttgcacttc
    28501 aattaattgt aagaccgctt cctgcatagc gttgctcata ttgaaattga cgcacaattg
    28561 accaggccca atttcccgta cgccagactc atcatcgctt aaaagcaagc cagaggcagt
    28621 aagattagac ggattatccg ttaaaaagat atcataatct tgactaatta atctttcatc
    28681 gggtgtgata caggttgcac cccaattttc taactggcga gtgacaatat tccgaatttc
    28741 tgccgaagta acatccacca ttacgcagac atcatccagt aatcgctctt cttcctcttc
    28801 aacttccggg tcagctgcga gcattttgat atgcacagag tagcgtgtac caagcccatc
    28861 ccgcgttttg atgtttaaat gaccgcccag tttacgcgcc agttgatcgc tcagccagaa
    28921 tgccagcggg tccgccttgc catagcgatc gttttgggtc tggttgataa acgggaagtg
    28981 caaattatcc atttcatgaa tgcttacgcc ttctccagtg tccagaatgc ggaacgtcag
    29041 gcggtcttcg gaggactcat cctgatcaac ctcaagggtg atttttccta attgcgttga
    29101 ggtcacggca tattgcatca gtagcagcaa aatacgtcgt aaggcatcgc gatcgccgcg
    29161 gcgcatatcg tgtgctttca gatgattgtt aatcagcagt tgcagacctt tacgcttgat
    29221 ggcaggcaac actgaaggca ctacttcatc aattaaatcc tgcacggaga acagctccgt
    29281 ctcacttttc cagctatcgt ccgcaagcat gttcgctaac tgtatttcat cgaccagtcg
    29341 caccagcaca tcagcctgat tcgccagttg tttgccttcc ggggcgttga gtttagccgc
    29401 gctctccgcc agggactgtg cgggttcttt cagcgcatcg ccaatgtttt tcataaaggt
    29461 catccgcccc tgctggtttt tctcatacag acgctgcgcc tgcttgagtt tcttgtttac
    29521 cagcacttcg cgatcctgat cgcgaataat gaaaatttgt gtgcgcggcg caacctggct
    29581 gcggaacatg cggatctcat acagctcgtt attgatcgtc gcctgaataa tcccctgatg
    29641 ctgttccgcc atggtggtga tgttttgcag attcaaatgc ggcagcaaat gatcggcaat
    29701 tttgttactt atgactgtgc ggtttgattc ctgatcgtga accagcaggc cgagcggcag
    29761 cagtgagact atctcttcat taattgcccg taaaatgcgc aattcgttat tgaccgccgt
    29821 gctggggacg ctttctgtac tgcggctgga gaaatggcgg aatgtggtat agccaaataa
    29881 cgccagcgcc agcaaaccga tgttcagcag cagtggcagc agaatgtttt gcaacgtatc
    29941 cagcagtaag gtgccgtaag gaacctgcca gaccaggcgc atatcggtag agttgagtgc
    30001 cgaggagatt tcaatcttcg tactgttaaa gtggatactg acgctatccg tcccttcttt
    30061 ctcattttca ttgtttcccg ttgccgtcgc gtctggctca aggcggaaac tgtccagcgg
    30121 catacccggt gggatcaaat cattaatcgg cagatcaaaa gccacgaccg ttgccagatg
    30181 tcccggctgg ttgaacgtgg tacgcaaggt aaagtaatga ccgttctgcc aggccaggcg
    30241 gcgcatgtta gaaaaacttt cgcgttcatc gagggcgttg gcctgttgca acatctctgc
    30301 acgacgtgaa tcaacaatgt cgctgacggt cgattcttta aatccggagg tgagatcttt
    30361 gaggggcagg gttgagatca gcaccagact gttatcctga ccattcaggt aatacatcga
    30421 ccacggtaca ttttctgcgc cccacaatgt gtccagatag gtggacatcc gttgagtcat
    30481 ctcaagcgtt gagttgtcgt gagagccaaa gattaacgct tccgttttgc ggcgtggttt
    30541 ctccagatag tagacatcct gtttcaggcg cgtctcttgt aaaccttcgc cggaggaggg
    30601 ggaggtcgtc gcggcaatgt tgtcgtagat ctgccaggtc acgtaacgcc aggtatcgac
    30661 gcgcttttgg attgcgtggg taatgtcgac aatctggtaa cttttatctt tcagccaggc
    30721 gttaacggcg ctttgtacca ttacacccat cgtcaccagt aacacaatga tcaacagtaa
    30781 aaagaagcgg gtaatgctcc ccggtaggag tgaaaagcgg gtcgtggccg ttgtctcttt
    30841 ctgacgcatt cgtgtttatg acctgttaaa acttcgcgaa gtgatagggc agtataaagg
    30901 gtacagtgtg aaattccaga ctcttacctg attgatgatt ctctattctc cagtgatatt
    30961 tatcatccgg cattggggga aatagatgta caagcgccat tttgctgtac aaataaccta
    31021 caaaaagccc aacgaaatta attgcaaata aacgaagatt gctggaaatt atgctgatgt
    31081 taattatttg tgaaatagtt aacaagcgtt atagtttttc tgtggtagca cagaataatg
    31141 aaaagtgtgt aaagaagggt aaaaaaaacc ggatgcgagg catccggttg aaataggggt
    31201 aaacagacat tcagaaatga atgacggtaa taaataaagt taatgatgat agcgggagat
    31261 attctagttg cgagtgaagg ttttgttttg a
//
LOCUS       DAHHTG010000050        31254 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.050, whole genome shotgun sequence.
ACCESSION   DAHHTG010000050 DAHHTG010000000
VERSION     DAHHTG010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 31254)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31254)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31254
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.050"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            45..803
                     /locus_tag="NBI74_002195"
     CDS             45..803
                     /locus_tag="NBI74_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001524287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HCD1932528.1"
                     /translation="MFLWFMQPDIWLTLITLTFLEIILGVDNIIFLSLVVAKLPLSQQ
                     NMARKLGLSCAMIMRILLLISIAWLSHITQPLFTVLTLEISFRTLILLGGGLFLIYKA
                     IQEIREELSPAQEDEHGSGSRQLSLCGAIVQIMLLDIVFSLDSVITAVGLSQHIFIMI
                     AAVMIAVGVMMFAAKTIGDFVNATPSIKILALTFLLFVGVLLVADSLNIHIAKEYLYF
                     AIFFSLSVETLNIIRERKNKKVSTLIETHESHSH"
     gene            839..2008
                     /locus_tag="NBI74_002196"
     CDS             839..2008
                     /locus_tag="NBI74_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000109428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exo-alpha-sialidase"
                     /protein_id="HCD1932529.1"
                     /translation="MSVALIRDGVIRPSENDSHVLTAMLPSSCPQNHAANILPLPDGA
                     LMCVWFAGTQEGIADISVWGSRLPAGGMQWSDAAKLSHDDTRSEQNPVLFLAPDNVLW
                     LLWTAQISGNQDTAIVRYRKSDDLGQTWGEIATLLDKPGTFIRQPITVLDNGNWLLPV
                     FYCRTQPGEKWVGNDDISAVKISADGGHSWRDVEVPQSLGCVHMNITMLHDGTLVALF
                     RSRWADNIYISHSVDNGESWSVPQATELPNNNSSIQVTTLASGELALVYNAMSAAGAV
                     ERRASLYDEIDDGDDSRKEPTAVGRSAFWGAPRAPMTVAISADGGKSWSWRRNLDEGD
                     GYCMTNNSLEKLNREFSYPSIKQSPDGTLHIAYTWWRQAIKYVRISPEWVKGQAS"
     gene            2005..2475
                     /locus_tag="NBI74_002197"
     CDS             2005..2475
                     /locus_tag="NBI74_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015964430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HCD1932530.1"
                     /translation="MIIGNLNHLSLAGLPAWVRNILLRPECSLSALSTREDGRWQPEG
                     CRWFCSLGTSDTQPAELRHTEYHHLWADIQVVITGCEGINAGTRPIARENDEERKPDL
                     FIAPSPENSVAITLHAGDFAVFMPEEPHQALCAIGTATSIRKAVFKVPRDMLEA"
     gene            2489..3769
                     /locus_tag="NBI74_002198"
     CDS             2489..3769
                     /locus_tag="NBI74_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000177666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion permease"
                     /protein_id="HCD1932531.1"
                     /translation="MTMEMILALGILVLMIVLIMSDKMPFGAPPLLACLLLVVSGLST
                     VQQAFAGFVNPSVVMIAGFMVVMAALQKTRLISNVKSAMISLVNKGSYRSYGLLLVIV
                     MLGASLAGTGATGYYVLILSLVSTIPYSKKLPTSKLMMPLGFATNHPLLPINLALLFG
                     VTATVLETAGFHQAISMGRFALVNLIMSAAFLAWSLIAYRFLPDHPIADASEDALAAR
                     EETFNALPAWKEYCTIAAFAVSVIGMMLMNILGNIAFVIPGLAGAFVLMIDVLDFKEV
                     RDHMGAPVILMMAGVIGVADALAGTGFTAMVGDAVAGVLGSGVSPFVFIVAFALLTST
                     CATFTGSNMGSVYIFAPIAIAACTSLGLNPTAAAIAVVISGWNGGYMPIDGMPAMILG
                     MGKYKLPEFWVFSVPMYLIRILALCAGAVFIFPM"
     gene            complement(3858..5804)
                     /gene="nagE"
                     /locus_tag="NBI74_002199"
     CDS             complement(3858..5804)
                     /gene="nagE"
                     /locus_tag="NBI74_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetyl glucosamine transporter subunit
                     IIABC"
                     /protein_id="HCD1932532.1"
                     /translation="MNILGFFQRLGRALQLPIAVLPVAALLLRFGQPDLLNVAFIAQA
                     GGAIFDNLALIFAIGVASSWSKDSAGAAALAGAVGYFVLTKAMVTINPEINMGVLAGI
                     ITGLVGGAAYNRWSDIKLPDFLSFFGGKRFVPIATGFFCLVLAAIFGYVWPPVQHAIH
                     AGGEWIVSAGALGSGIFGFINRLLIPTGLHQVLNTIAWFQIGEFTNAAGTVFHGDINR
                     FYAGDGTAGMFMSGFFPIMMFGLPGAALAMYFAAPKERRPMVGGMLLSVAVTAFLTGV
                     TEPLEFLFMFLAPLLYLLHALLTGISLFVATLLGIHAGFSFSAGAIDYALMYNLPAAS
                     QNVWMLLVMGVVFFAIYFVVFSLVIRMFNLKTPGREDKEDEIVTEEANSNTEEGLNQL
                     ATNYIAAVGGTDNLKAIDACITRLRLTVVDSARVNDAMCKRLGASGVVKLNKQTIQVI
                     VGAKAESIGDAMKKVVARGPVAAASAEATPATAAPVAKPQAVPNAVSIAELVSPITGD
                     VVALDQIPDEAFASKAVGDGVAVKPTDKIVVSPAAGTIVKIFNTNHAFCLETEKGAEI
                     VVHMGIDTVALEGKGFKRLVEEGAQVSAGQPILEMDLDYLNANARSMISPVVCSNIDD
                     FSGLIIKAQGHVVAGQTPLYEIKK"
     gene            6137..6937
                     /gene="nagB"
                     /locus_tag="NBI74_002200"
     CDS             6137..6937
                     /gene="nagB"
                     /locus_tag="NBI74_002200"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008805656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HCD1932533.1"
                     /translation="MRLIPLTTAEQVGKWAARHIVNRINAFKPTADRPFVLGLPTGGT
                     PMTTYKALVEMHKAGQVSFKHVVTFNMDEYVGLPKEHPESYYSFMHRNFFDHVDIPAE
                     NINLLNGNAPDIDAECRQYEEKIRSYGKIHLFMGGVGNDGHIAFNEPASSLASRTRIK
                     TLTHDTRVANSRFFDNDVNQVPKYALTVGVGTLLDAEEVMILVLGSQKALALQAAVEG
                     CVNHMWTISCLQLHPKAIMVCDEPSTMELKVKTLRYFNELEAENIKGL"
     gene            6997..8145
                     /gene="nagA"
                     /locus_tag="NBI74_002201"
     CDS             6997..8145
                     /gene="nagA"
                     /locus_tag="NBI74_002201"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HCD1932534.1"
                     /translation="MYALTQGRIFTGHEFLDDHAVVIADGLIKSVCPVAELPPEIEQR
                     SLNGAILSPGFIDVQLNGCGGVQFNDTAEAVSVETLEIMQKANEKSGCTNYLPTLITT
                     SDELMKQGVRVMREYLAKHPNQALGLHLEGPWLNLVKKGTHNPNFVRKPDAALVDFLC
                     ENADVITKVTLAPEMVPAEVISKLANAGIVVSAGHSNATLKEAKAGFRAGITFATHLY
                     NAMPYITGREPGLAGAILDEADIYCGIIADGLHVDYANIRNAKRLKGDKLCLVTDATA
                     PAGANIEQFIFAGKTIYYRNGLCVDENGTLSGSSLTMIEGVRNLVEHCGIALDEVLRM
                     ATLYPARAIGVEKRLGTLAAGKVANLTAFTPDFKITRTIVNGNEVVTQ"
     gene            8154..9374
                     /gene="nagC"
                     /locus_tag="NBI74_002202"
                     /gene_synonym="nagR"
     CDS             8154..9374
                     /gene="nagC"
                     /locus_tag="NBI74_002202"
                     /gene_synonym="nagR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NagC"
                     /protein_id="HCD1932535.1"
                     /translation="MTPGGQAQIGNVDLVKQLNSAAVYRLIDQYGPISRIQIAEQSQL
                     APASVTKITRQLIERGLIKEVDQQASTGGRRAISIVTETRNFHAIGVRLGRHDATITL
                     FDLSSKVLAEEHYPLPERTQQTLEHALLNAIAQFIDSYQRKLRELIAISVILPGLVDP
                     DSGKIHYMPHIQVENWGLVEALEERFKVTCFVGHDIRSLALAEHYFGASQDCEDSILV
                     RVHRGTGAGIISNGRIFIGRNGNVGEIGHIQVEPLGERCHCGNFGCLETIAANAAIEQ
                     RVLNLLKQGYQSRVPLDDCTIKTICKAANKGDSLASEVIEYVGRHLGKTIAIAINLFN
                     PQKIVIAGEITEADKVLLPAIESCINTQALKAFRTNLPVVRSELDHRSAIGAFALVKR
                     AMLNGILLQHLLEN"
     gene            9422..10174
                     /gene="nagD"
                     /locus_tag="NBI74_002203"
     CDS             9422..10174
                     /gene="nagD"
                     /locus_tag="NBI74_002203"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide monophosphatase NagD"
                     /protein_id="HCD1932536.1"
                     /translation="MTIKNVICDIDGVLMHDNVAVPGAAEFLHGIMDKGLPLVLLTNY
                     PSQTGQDLANRFATAGVDVPDSVFYTSAMATADFLRRQEGKKAYVVGEGALIHELYKA
                     GFTITDVNPDFVIVGETRSYNWDMMHKAAYFVANGARFIATNPDTHGRGFYPACGALC
                     AGIEKISGRKPFYVGKPSPWIIRAALNKMQAHSEETVIVGDNLRTDILAGFQAGLETI
                     LVLSGVSSLDDIDSMPFRPSWIYPSVAEIDVI"
     gene            10430..12094
                     /gene="asnB"
                     /locus_tag="NBI74_002204"
     CDS             10430..12094
                     /gene="asnB"
                     /locus_tag="NBI74_002204"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001724523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase B"
                     /protein_id="HCD1932537.1"
                     /translation="MCSIFGVFDIKTDAVELRKKALELSRLMRHRGPDWSGIYASDNA
                     ILAHERLSIVDVNAGAQPLYNQQKTHVLAVNGEIYNHQALRAEYGDRYQFQTGSDCEV
                     ILALYQEKGPEFLDDLQGMFAFALYDSEKDAYLIGRDHLGIIPLYMGYDEHGQLYVAS
                     EMKALVPVCRTIKEFPAGSYLWSQDGEIRSYYHRDWFDYDAVKDNVTDKNELRQALED
                     SVKSHLMSDVPYGVLLSGGLDSSIISAITKKYAARRVEDQERSEAWWPQLHSFAVGLP
                     GSPDLKAAQEVANHLGTVHHEIHFTVQEGLDAIRDVIYHIETYDVTTIRASTPMYLMS
                     RKIKAMGIKMVLSGEGSDEVFGGYLYFHKAPNAKELHEETVRKLLALHMYDCARANKA
                     MSAWGVEARVPFLDKKFLDVAMRINPQDKMCGNGKMEKHILRECFEAYLPASVAWRQK
                     EQFSDGVGYSWIDTLKEVAAQQVSDQQLETARFRFPYNTPTSKEAYLYREIFEELFPL
                     PSAAECVPGGPSVACSSAKAIEWDEAFKKMDDPSGRAVGVHQSAYK"
     gene            complement(12136..12573)
                     /locus_tag="NBI74_002205"
     CDS             complement(12136..12573)
                     /locus_tag="NBI74_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000943401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1932538.1"
                     /translation="MLRFMTALMGALLLMQSAFADTVKPEIGKYVFGYRGQEGAVVWM
                     MRIGPKASNEALIQVSHVDNDIDGQIFRCKVKALQEGEKSYTAVIKGKSFELLRLKEG
                     NGRLHIPDEQATWLVAYSDELSNSDVANPEHFLTAYQNQQAPQ"
     gene            12906..12982
                     /locus_tag="NBI74_002206"
     tRNA            12906..12982
                     /locus_tag="NBI74_002206"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12940..12942,aa:Met,seq:cat)
     gene            12993..13077
                     /locus_tag="NBI74_002207"
     tRNA            12993..13077
                     /locus_tag="NBI74_002207"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:13027..13029,aa:Leu,seq:tag)
     gene            13101..13175
                     /locus_tag="NBI74_002208"
     tRNA            13101..13175
                     /locus_tag="NBI74_002208"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:13133..13135,aa:Gln,seq:ttg)
     gene            13210..13284
                     /locus_tag="NBI74_002209"
     tRNA            13210..13284
                     /locus_tag="NBI74_002209"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:13242..13244,aa:Gln,seq:ttg)
     gene            13300..13376
                     /locus_tag="NBI74_002210"
     tRNA            13300..13376
                     /locus_tag="NBI74_002210"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:13334..13336,aa:Met,seq:cat)
     gene            13425..13499
                     /locus_tag="NBI74_002211"
     tRNA            13425..13499
                     /locus_tag="NBI74_002211"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:13457..13459,aa:Gln,seq:ctg)
     gene            complement(13654..14829)
                     /gene="ubiF"
                     /locus_tag="NBI74_002212"
     CDS             complement(13654..14829)
                     /gene="ubiF"
                     /locus_tag="NBI74_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-3-methyl-6-methoxy-1,4-benzoquinol
                     hydroxylase"
                     /protein_id="HCD1932539.1"
                     /translation="MTNQPTEIAIVGGGMVGGALALGLAQHGFTVTVIEHAEPAPFVA
                     DSQPDVRISAISAASVSLLKGLGVWDAVQAMRCHPYRRLETWEWETAHVVFDAAELKL
                     PLLGYMVENTVLQQALWQALEAHPKVTLRVPTSLTALHRHNDLQELELKGGETILAKL
                     VIGADGANSQVRQMAGIGVHAWQYAQSCMLISVQCENDPGDSTWQQFTPDGPRAFLPL
                     FDNWASLVWYDSPARIRQLQNMNMAQLQAEIAKHFPSRLGYVTPLAAGAFPLIRRHAL
                     QYVQPGLALVGDAAHTIHPLAGQGVNLGYRDVDALIDVLVNARSYGEAWASYPVLKRY
                     QMRRMADNFMMQSGMDLFYAGFSNNLPPLRFVRNLGLMAAERAGVLKRKALKYALGL"
     gene            14975..16399
                     /gene="miaB"
                     /locus_tag="NBI74_002213"
     CDS             14975..16399
                     /gene="miaB"
                     /locus_tag="NBI74_002213"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HCD1932540.1"
                     /translation="MTKKLHIKTWGCQMNEYDSSKMADLLDATHGYQLTDVAEEADVL
                     LLNTCSIREKAQEKVFHQLGRWKLLKEKNPDLIIGVGGCVASQEGEHIRQRAHYVDII
                     FGPQTLHRLPEMINSVRGDRSPVVDISFPEIEKFDRLPEPRAEGPTAFVSIMEGCNKY
                     CTYCVVPYTRGEEVSRPSDDILFEIAQLAAQGVREVNLLGQNVNAWRGENYDGTTGTF
                     ADLLRLVAAIDGIDRIRFTTSHPIEFTDDIIEVYRDTPELVSFLHLPVQSGSDRILNL
                     MGRTHTALEYKAIIRKLRAARPDIQISSDFIVGFPGETTDDFEKTMKLIADVNFDMSY
                     SFIFSARPGTPAADMVDDVPEEEKKQRLYILQERINQQAMAWSRRMLGTTQRILVEGT
                     SRKSIMELSGRTENNRVVNFEGTPDMIGKFVDVEITDVYPNSLRGKVVRTEDEMGLRV
                     AETPESVIARTRKENDLGVGYYQP"
     gene            16552..17592
                     /locus_tag="NBI74_002214"
     CDS             16552..17592
                     /locus_tag="NBI74_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415193.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HCD1932541.1"
                     /translation="MNIDTREITLEPADNARLLSLCGPFDDNIKQLERRLGIEINRRD
                     NHFKLTGRPICVTAAADILRSLYVDTAPMRGQIQDIEPEQIHLAIKEARVLEQSAESV
                     PEYGKAVNIKTKRGVIKPRTPNQAQYIANILDHDITFGVGPAGTGKTYLAVAAAVDAL
                     ERQEIRRILLTRPAVEAGEKLGFLPGDLSQKVDPYLRPLYDALFEMLGFEKVEKLIER
                     NVIEVAPLAYMRGRTLNDAFIILDESQNTTIEQMKMFLTRIGFNSKAVITGDVTQIDL
                     PRNTKSGLRHAIEVLADVEEISFNFFHSEDVVRHPVVARIVNAYEAWEEAEQKRKAAL
                     AAERKREEQEQK"
     gene            17589..18056
                     /gene="ybeY"
                     /locus_tag="NBI74_002215"
     CDS             17589..18056
                     /gene="ybeY"
                     /locus_tag="NBI74_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014169133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HCD1932542.1"
                     /translation="MSQVILDLQLACEDNSGLPEESQFQTWLNAVIPQFQEESEVTIR
                     VVDTAESHSLNLTYRGKDKPTNVLSFPFEVPPGMEMSLLGDLVICRQVVEKEAQEQGK
                     PLEAHWAHMVVHGSLHLLGYDHIEDDEAEEMEAIETEIMLALGYEDPYIAEKE"
     gene            18146..19024
                     /gene="corC"
                     /locus_tag="NBI74_002216"
     CDS             18146..19024
                     /gene="corC"
                     /locus_tag="NBI74_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308723.1"
                     /note="CorC(YbeX) belongs to the Cyclin M Mg2+ Exporter
                     (CNNM) family, and was characterized as belonging to a set
                     of three proteins, at least one of which must be present
                     for CorA to function; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family magnesium/cobalt transport protein
                     CorC"
                     /protein_id="HCD1932543.1"
                     /translation="MSDDNSHSSDTISNKKGFFSLLLSQLFHGEPKNRDELLALIRDS
                     GQNDLIDEDTRDMLEGVMDIADQRVRDIMIPRSQMITLKRNQTLDECLDVIIESAHSR
                     FPVISEDKDHIEGILMAKDLLPFMRSDAEAFSMDKVLRQAVVVPESKRVDRMLKEFRS
                     QRYHMAIVIDEFGGVSGLVTIEDILELIVGEIEDEYDEEDDIDFRQLSRHTWTVRALA
                     SIEDFNEAFGTHFSDEEVDTIGGLVMQAFGHLPARGETIDIDGYQFKVAMADSRRIIQ
                     VHVKIPDDSPQPKLDE"
     gene            19049..20587
                     /gene="lnt"
                     /locus_tag="NBI74_002217"
     CDS             19049..20587
                     /gene="lnt"
                     /locus_tag="NBI74_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HCD1932544.1"
                     /translation="MAFASLIERQRIRLLLALLFGACGTLAFSPYDVWPAAIISLMGL
                     QALTFNRRPLQSAAIGFCWGFGLFGSGINWVYVSIATFGGMPGPVNIFLVVLLAAYLS
                     LYTGLFAGVLSRLWPKTTWLRVAIAAPALWQVTEFLRGWVLTGFPWLQFGYSQIDGPL
                     KGLAPIMGVEAINFLLMMVSGLLALALVKRNWRPLVVAVVLFALPFPLRYVQWFTPQP
                     EKTIQVSMVQGNIPQSLKWDEGQLLNTLKIYYNATAPLMGKSSLIIWPESAITDLEIN
                     QQPFLKALDGELRDKGSSLITGIVDARLNKQNRYDTYNTIITLGKGASYSYESADRYN
                     KNHLVPFGEFVPLESILRPLAPFFDLPMSSFSRGPYIQPPLSLNGIQLTAAICYEIIL
                     GEQVRDNFRPDTDYLLTISNDAWFGKSIGPWQHFQMARMRALELARPLLRSTNNGITA
                     VIGPQGEIQAMIPQFTREVLTTNVTPTTGLTPYARTGNWPLWVLTALFGFAAVLMSLR
                     ARKR"
     gene            complement(20714..22591)
                     /locus_tag="NBI74_002218"
     CDS             complement(20714..22591)
                     /locus_tag="NBI74_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001276742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HCD1932545.1"
                     /translation="MSASSVKPLNVQLPAITLILFALCVGIFCYLAQWMSYEEVDQSA
                     LIHLGANVASLTLSDESWRLLSSVFLHSSFSHLLMNMFALLVVGAVAERILGKWRLLI
                     IWLFSGVFGGLISACYALRESEQIVISVGASGAIMGIAGAAIATQLASGAGTHHKNQR
                     RVFPLLGMVALTLLYGTRQTGVDNACHIGGLIAGGALGWLSARLVGQNRLVTEGGIIV
                     AVTLLLTGTIWLVQQQIDESVLQVRQSLREAFYPQEIEQERRQKKQQLVEERNALRET
                     LSAPVSREQASGDLLAEIADIHDMAISRDANTLYAAIENTNSIVVFDLGQKKILHTFT
                     APIAKEKSVKHCGGCKDQGVRSLALSPDETLLYATSFEANALSVINVATGEIIQSITT
                     GAHPDSLILSRDGTKAWVMNRTSNSVSAIDLVAYQHVVDIPLEKYDGTGMSGKPGAWV
                     MALSPNEKTLLVPGAGRGNIVRINTITHQKEDFPAGDARGVVSAMGFRPKNGEIIFAD
                     SQGISRIRAEDQQASIMTQWCSRSVYSVEGISPDGQYLALVSYGLQGYVILLNINAGQ
                     IIGVYPASYVNHLRFSADGRKIFVMAKNGLIQLDRESSFDPQAIIRHPQYGDVACIPE
                     P"
     gene            23045..23953
                     /gene="gltI"
                     /locus_tag="NBI74_002219"
     CDS             23045..23953
                     /gene="gltI"
                     /locus_tag="NBI74_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter
                     substrate-binding protein GltI"
                     /protein_id="HCD1932546.1"
                     /translation="MQLRKPATAILALALSAGLAQADDAAPAAGNTLDKIAKNGVIVV
                     GHRESSVPFSYYDNQQKVVGYSQDYSNAIVEAVKKKLNKPDLQVKLIPITSQNRIPLL
                     QNGTFDFECGSTTNNAERQKQAAFSNTIFVVGTRLLTKKGGDIKDFADLKGKAVVVTS
                     GTTSEVLLNKLNEEQKMNMRIISAKDHGDSFRTLESGRAVAFMMDDALLAGERAKAKK
                     PDNWEIVGKPQSQEAYGCMLRKDDPQFKKLMDDTIAQVQTSGEAEKWFDKWFKNPIPP
                     KNLNMNFELSDEMKALFKEPNDKALN"
     gene            24123..24863
                     /gene="gltJ"
                     /locus_tag="NBI74_002220"
     CDS             24123..24863
                     /gene="gltJ"
                     /locus_tag="NBI74_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltJ"
                     /protein_id="HCD1932547.1"
                     /translation="MSIDWNWGIFLQQAPFGNTTYLGWIWSGFQVTIALSICAWIIAF
                     LVGSFFGILRTVPNRFLSGLGTLYVELFRNVPLIVQFFTWYLVIPELLPEKIGMWFKA
                     ELDPNIQFFLSSMLCLGLFTAARVCEQVRAAIQSLPRGQKNAALAMGLTLPQAYRYVL
                     LPNAYRVIVPPMTSEMMNLVKNSAIASTIGLVDMAAQAGKLLDYSAHAWESFTAITLA
                     YVLINAFIMLVMTLVERKVRLPGNMGGK"
     gene            24863..25537
                     /gene="gltK"
                     /locus_tag="NBI74_002221"
     CDS             24863..25537
                     /gene="gltK"
                     /locus_tag="NBI74_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltK"
                     /protein_id="HCD1932548.1"
                     /translation="MYEFDWSSIVPSLPYLLDGLVITLKITVTAVVIGILWGTMLAVM
                     RLSSFAPVAWFAKAYVNVFRSIPLVMVLLWFYLIVPGFLQNVLGLSPKNDIRLISAMV
                     AFSMFEAAYYSEIIRAGIQSISRGQSSAALALGMTHWQSMKLIILPQAFRAMVPLLLT
                     QGIVLFQDTSLVYVLSLADFFRTASTIGERDGTQVEMILFAGFVYFVISLSASLLVSY
                     LKRRTA"
     gene            25537..26262
                     /gene="gltL"
                     /locus_tag="NBI74_002222"
     CDS             25537..26262
                     /gene="gltL"
                     /locus_tag="NBI74_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020245804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter ATP binding
                     protein GltL"
                     /protein_id="HCD1932549.1"
                     /translation="MITLKNVSKWYGHFQVLTDCSTEVKKGEVVVVCGPSGSGKSTLI
                     KTVNGLEPVQQGEITVDGIVVNDKKTDLAKLRSRVGMVFQHFELFPHLSIIENLTLAQ
                     VKVLKRNKAPAREKALKLLERVGLSAHANKFPAQLSGGQQQRVAIARALCMDPIAMLF
                     DEPTSALDPEMINEVLDVMVELANEGMTMMVVTHEMGFARKVANRVIFMDEGKIIEDS
                     PKDAFFDNPKSDRAKDFLAKILH"
     gene            26380..27315
                     /gene="rihA"
                     /locus_tag="NBI74_002223"
     CDS             26380..27315
                     /gene="rihA"
                     /locus_tag="NBI74_002223"
                     /EC_number="3.2.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-specific ribonucleoside hydrolase
                     RihA"
                     /protein_id="HCD1932550.1"
                     /translation="MALPILLDCDPGHDDAIAIVLALASPELDVKAITSSAGNQTPEK
                     TLRNVLRMLTLLNRTDIPVASGAVKPLMRNLIIADNVHGESGLDGPALPEPAFAPQNC
                     TAVELMAKTLRESAEPVTIVSTGPQTNVALLLNSHPELHSKIARIVIMGGAMGLGNWT
                     PAAEFNIYVDPEAAEIVFQSGIPVVMAGLDVTHKAQIHVEDTERFRAIGNPVSTIVAE
                     LLDFFLEYHKDEKWGFVGAPLHDPCTIAWLLKPELFTTVERWVGVETQGKYTQGMTVV
                     DYYYLTGNKPNATVMVDVDRQGFVDLLADRLKFYA"
     gene            27399..29069
                     /gene="hscC"
                     /locus_tag="NBI74_002224"
     CDS             27399..29069
                     /gene="hscC"
                     /locus_tag="NBI74_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HscC"
                     /protein_id="HCD1932551.1"
                     /translation="MDNAELAIGIDLGTTNSLIAVWKDGAAQLIPNKFGEYLTPSIIS
                     MDENNHILVGKPAVSRRTSHPDKTAALFKRAMGSNTNWRLGSDTFNAPELSSLVLRSL
                     KEDAEEFLQRPIKDVVISVPAYFSDEQRKHTRLAAELAGLNAVRLINEPTAAAMAYGL
                     HTQQNTRSLVFDLGGGTFDVTVLEYATPVIEVNASAGDNFLGGEDFTHMLVDEVLKRA
                     EVAKTTLNDSELAALYACVEAAKCSNQSPMHIRWQYQDEMRKCEFYENELEDLWLPLL
                     NRLRVPIEQALRDARLKPSQIDSLVLVGGASQMPLVQRIAVRLFGKLPYQSYDPSTIV
                     ALGAAIQAACRLRNEDIEEVILTDICPYSLGVEVNRQGVSGIFSPIIERNTTVPVSRV
                     ETYSTMHPEQDSITVNVYQGENHKVKNNILVESFDVPLKKTGTYQSIDIRFSYDINGL
                     LEVDVLLEDGSVKSRVINHSPVTLSAQQIEESRTRLSALKIYPRDMLINRTFKAKLEE
                     LWARALGDEREEIGRVITDFDAALQSNDMARVDEVRRRASDYLAIEIP"
     gene            complement(29129..30580)
                     /gene="djlC"
                     /locus_tag="NBI74_002225"
     CDS             complement(29129..30580)
                     /gene="djlC"
                     /locus_tag="NBI74_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlC"
                     /protein_id="HCD1932552.1"
                     /translation="MKTCWQILEIESTTQIDIIRQAYLARLPLCHPETDPQGFKALRQ
                     AYEEALRLAVNPVEEADDEEKDAATEHEILRVFRALLDSENDRFQPSAWQKFIQQLNT
                     WNMEDVDQLRWPLCAIAIEARYLSLNCASLLAERLNWHSFNDCEGIDEEEREAFLEAI
                     QAGDCFDFLSLLEYPVALQNQTVEYYFSLERCCRYHPDYVTAFLAMEGPWFIPDDAKL
                     HRKLLRWYSSVQTGMAELIPVAQQWQAEEPESEDARYYQCAQRLYCGEGESLLADLCA
                     YRESYPSTQADNLLLQWSKRHCPDYFALLVMVIEARSMVDAQGKPLKYVPGESARTRL
                     LWAEILHSGKLSPLGQSFIESLFFKRKAWAWWKSRVGSETEQDSPLLDLYRVAEQVVL
                     EAFPKQEMLARLDARLERGEAHPLETIITRMLLTKVKLEPEDEDVDEPTPENHEEIND
                     EDEKPQSITSIIKISLTVLVIGYALGKIAMLFS"
     gene            complement(30577..>31254)
                     /locus_tag="NBI74_002226"
     CDS             complement(30577..>31254)
                     /locus_tag="NBI74_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HCD1932553.1"
                     /translation="ALSTPMEILNQGCKPAHDSPKMYTGIKEFDLSSSWGINNRDDLI
                     QTIYQMTDDGHANDLAGLYLTWHRSSPEEWKALIAGGSERGLIYTQFVAQTAMCCGEG
                     GIKAWDYVRMGFLSRVGVLNKWLTEDESLWLQSRVYIRAHHYYHSWMHYFSAYSLGRL
                     YWQSSQCEDNASLREALTLYKYDSAGSRMFEELAAGSDRFYATLPWQPLTVQPECPVT
                     LKDVSDL"
BASE COUNT         7657 a         8117 c         7866 g         7614 t
ORIGIN      
        1 cagcccgcta taaagtttag ataaagagaa taaaggatta ccccatgttt ttgtggttta
       61 tgcagccaga tatctggctc actttaatta ccctcacctt tctggaaatt attcttgggg
      121 tggataatat tatttttctt tcactggtgg tggcaaaact tccgttatca caacaaaata
      181 tggcgcgtaa actgggttta agctgcgcga tgattatgcg gatattattg ctgatatcca
      241 ttgcgtggct ttcacatatt acccagccgc tttttaccgt gctgacgctg gagatttcgt
      301 tccgcacgtt gattctgctc gggggcgggc tgttcctgat ttataaagcg atacaggaga
      361 tacgcgaaga gctgtcgccc gcgcaggaag acgaacatgg cagcggatcg cggcaactgt
      421 cgttgtgcgg tgccattgta cagataatgc tgctcgatat cgtgtttagc ctggattcgg
      481 tgatcaccgc cgtcggcctt tctcagcata tatttatcat gattgccgcc gtaatgattg
      541 ccgtcggtgt catgatgttt gcggcgaaaa caattggtga tttcgttaac gccacgccgt
      601 caataaaaat actggcgtta acctttttat tgtttgtcgg tgtattgctg gttgccgata
      661 gtctcaacat tcatattgcc aaagagtatc tctacttcgc gatatttttc tcattaagtg
      721 ttgagacact caatattatc cgtgaaagaa aaaataaaaa agtttcgaca ttgatcgaaa
      781 cgcatgaatc gcactcgcat taataaatct attaccaact ttaagatgga gtctaactat
      841 gtctgtagcc cttattcgtg acggcgtcat ccgtccatcc gaaaacgatt cgcatgtgct
      901 taccgccatg ctgccctcgt cctgcccgca aaaccacgcg gcgaatattc tgcctttgcc
      961 ggatggcgcg ttaatgtgcg tctggtttgc gggaacgcag gaaggtattg ccgatatttc
     1021 tgtctgggga tcgcgattgc ccgctggcgg aatgcagtgg agcgatgcgg ccaaactttc
     1081 ccacgatgac acgcgttcag agcaaaaccc ggtgctgttt ctcgcgccgg ataacgtgct
     1141 gtggctccta tggaccgcac aaatatccgg taatcaggac accgccatcg tccgctaccg
     1201 taagtcagac gacctcggac aaacctgggg cgagattgcc accctgcttg ataaacccgg
     1261 tacgttcatt cgacagccaa tcacggtgct tgataacggc aactggctgc tgcccgtctt
     1321 ttattgccgt acccaacccg gcgaaaaatg ggtcggcaat gatgatatca gcgcagtgaa
     1381 aatttccgct gacggtggtc actcctggcg cgatgtcgag gtgccgcaaa gtctcggctg
     1441 tgttcatatg aatatcacaa tgttgcacga cggtacgttg gttgctctct ttcgcagccg
     1501 ttgggcagac aacatctata tcagccattc agtggacaac ggcgagagtt ggtcggtgcc
     1561 gcaagcaacc gaattgccca acaacaattc gtctattcag gtaacgacgc ttgccagcgg
     1621 cgaactggcg ctggtgtaca acgcgatgag tgcggcaggc gcagtagaac gtcgcgcgtc
     1681 gctttatgac gaaatcgacg atggcgacga cagccgcaaa gaaccgacag ccgtcggacg
     1741 ctccgctttc tggggtgccc cacgcgcgcc gatgacggta gcgatttctg ccgatggtgg
     1801 gaaaagctgg tcgtggcggc gcaatctcga tgaaggcgac ggctactgca tgaccaacaa
     1861 ttccctggaa aagctaaatc gtgagttctc ctaccccagc atcaaacaaa gcccggacgg
     1921 tacgctacat atcgcttaca cctggtggcg tcaggccatc aagtacgtac gcatctcccc
     1981 cgagtgggtt aaggggcagg catcatgatt atcggtaatc tcaaccattt gtcgctggcg
     2041 gggcttcctg cctgggtgag aaatattctg ctgcgcccgg agtgttcgtt atcggcgtta
     2101 tccaccagag aggacggtcg ctggcaacct gaaggatgtc ggtggttctg ctcgctggga
     2161 acttcagaca cgcaacccgc cgaacttcgc catacggagt atcaccatct atgggccgat
     2221 atacaggtgg tgattactgg ttgtgagggc attaatgcgg gaacgcggcc catcgctcgc
     2281 gaaaacgatg aagaacgtaa gcccgatctg tttatcgccc catcccctga aaacagtgtc
     2341 gccatcaccc tacacgccgg tgatttcgcc gtctttatgc ctgaggaacc tcaccaggca
     2401 ctgtgcgcaa ttggcacggc tacttcaata cgcaaagcgg tctttaaagt accgcgcgac
     2461 atgctggaag cctaaaagga gagaaaaaat gacaatggaa atgatcctgg cattaggaat
     2521 actggtcctg atgatcgtgc tgatcatgtc ggacaaaatg ccttttggcg caccgccact
     2581 gcttgcgtgt ttattgctgg ttgtatcggg actttcgacc gttcaacagg cttttgccgg
     2641 attcgtcaat ccaagcgtgg tgatgattgc agggtttatg gtggtcatgg cggcgctaca
     2701 aaaaacccgg cttatcagca acgttaaatc tgcgatgata agcctggtca ataaaggcag
     2761 ttaccgcagc tatgggctgt tgttagtcat tgttatgctg ggtgccagtc ttgccggcac
     2821 cggagcgacc ggttattacg tgctaattct gtcgctggta tccaccattc cgtacagtaa
     2881 aaagctacca acatcgaaat taatgatgcc cctgggattt gccaccaacc acccgcttct
     2941 tcctatcaat ctcgcgcttc tctttggcgt tacggcgacc gtactcgaaa ccgcggggtt
     3001 tcatcaggcg atttcgatgg ggcgctttgc gctggtgaat ctcatcatgt cagcggcttt
     3061 cctcgcatgg agtcttattg cctaccgttt tcttccggat catcccattg cggatgcttc
     3121 cgaggatgcg ctggcggcac gcgaggaaac gtttaacgcc cttcctgcct ggaaagaata
     3181 ttgcactatt gccgcattcg ctgtcagcgt tatcggcatg atgctaatga acattctggg
     3241 aaatatcgcc ttcgttattc cgggtcttgc gggtgcgttt gttctgatga tcgatgtact
     3301 ggacttcaaa gaagttcgcg atcatatggg ggctccggtc attttaatga tggcgggggt
     3361 gatcggtgta gctgatgcac tggcgggcac cggctttacg gcgatggttg gcgatgccgt
     3421 tgcaggtgtg ctcggttctg gcgtctcacc gtttgtattt attgtcgcct ttgctttgct
     3481 taccagtacc tgcgccacct tcacgggttc gaatatgggg tcagtatata tttttgcccc
     3541 catcgccatt gccgcctgta ctagccttgg gcttaacccg accgctgccg cgattgcggt
     3601 ggtgatttct ggctggaatg gcggctatat gcctattgac ggaatgccag ccatgatcct
     3661 cggaatgggg aaatacaaac ttcccgaatt ctgggtgttc tctgtaccaa tgtatctcat
     3721 ccgcattctg gcgctttgtg caggcgctgt ttttatcttc ccaatgtaat ctcagacaaa
     3781 aaaagcggca gagaagatcc ctgccgcttt ttattgttgg atgcgacgct gccgcgtcgc
     3841 atcagacata aagcagatta ctttttgatt tcatacagcg gtgtttgacc cgccacaaca
     3901 tggccctgag ctttaatgat aaggccgctg aaatcatcga tattgctgca aaccaccggg
     3961 ctaatcatcg aacgggcgtt agcgttcagg taatccagat ccatttccag aatcggttgc
     4021 cctgcgctta cctgcgcgcc ctcttccacc agacgtttaa agcctttacc ttccagcgct
     4081 acggtgtcga tacccatatg gacgacgatc tccgcgcctt tttcggtttc cagacagaac
     4141 gcgtggttgg tgttgaagat tttcacgatt gttcctgcgg ctggtgatac gacgatttta
     4201 tctgtcggtt tcaccgccac accgtcaccc accgctttgc tggcgaatgc ttcgtcagga
     4261 atctgatcca gtgccacaac atccccggta atcggcgata ccagctccgc gatagatacc
     4321 gcgtttggta cagcctgcgg ttttgctaca ggagcggcag ttgccggagt tgcttcagct
     4381 gacgcagcgg ctaccggacc acgggcaacg actttcttca tcgcatcgcc gatggattct
     4441 gctttcgcgc caacaatcac ctgaatagtc tgtttgttca gttttactac cccagaagca
     4501 cccagacgtt tacacatcgc atcgttgacg cgagctgagt caaccacggt aaggcgcaga
     4561 cgagtaatac aggcgtcaat ggctttcagg ttgtcagtac cgccaaccgc agcaatatag
     4621 ttggttgcca gttgattgag accttcttca gtattgctgt tagcctcttc agtaacgatc
     4681 tcgtcttctt tatcttcacg acccggcgtt ttcaggttga acatgcggat aaccaaactg
     4741 aacaccacga agtagatagc gaagaagaca acccccatca ccagcagcat ccagacgttc
     4801 tggctggcgg ccggcaggtt atacatcaac gcgtagtcga tagcccccgc agagaaagag
     4861 aagccggcat ggatacccag caacgttgcc acaaacaggc tgataccggt cagcagtgcg
     4921 tgcaggaggt acagcagcgg tgcaaggaac atgaacagga attccagcgg ctcagtcaca
     4981 ccggtcagga acgcagtaac agcaacagaa agcagcatcc cgccaaccat cggacgacgc
     5041 tctttcggtg ctgcgaagta catcgccagc gccgcacccg gcaggccgaa catcataatc
     5101 gggaagaagc cggacatgaa catccccgcg gtgccgtcac cggcatagaa gcggttgata
     5161 tcaccgtgga aaaccgttcc cgccgcgttg gtgaattcac caatctggaa ccaggcgatg
     5221 gtgttcagca cctgatgcag accggttggg atcagcagac ggttgatgaa accaaagata
     5281 ccggaaccca gcgcgcccgc agaaacgatc cactcgccgc ctgcatggat agcgtgctgt
     5341 accggtggcc agacgtaacc aaaaatggcc gccagcacca gacagaagaa gccggtggct
     5401 atcggcacaa agcgtttgcc gccgaagaag ctcaggaagt ccggcagttt aatatcggac
     5461 caacggttat aggctgcgcc accaaccaga ccggtaatga tacccgccag tacacccatg
     5521 ttaatttctg ggttgatggt caccatcgct ttggttaaca caaagtaacc taccgcaccc
     5581 gccagtgccg ccgcacctgc gctgtctttc gaccagctgg atgccacgcc gatggcgaat
     5641 attaatgcga ggttatcaaa aatcgcaccg cccgcctggg caataaacgc aacgttaagt
     5701 aaatctggct gaccgaatcg cagcaacagc gccgccaccg gcagcaccgc gatagggagc
     5761 tgtaacgccc taccgagtcg ctggaaaaaa cctaaaatat tcatcttatt ccccctacga
     5821 gaaccctatt tggctcgttt aaagccgtat ttttattttg ctgcaaattg tactgccgat
     5881 gttctgtaat cagattgtta gatcatctgc tacagagtgt gtgaaaattt aattcgtatc
     5941 gcaaattaaa cgcgtgtctt ttgtgagttt tgtcaccaaa tatcgttatc atcactccct
     6001 tttactggct aaaccagaaa acttatttta tcattcaaaa aatcaggtcg gattgacgcc
     6061 tgtctgcgca aatccaggtt acgcttaaag atgcctaatc cgccaacggc ttacatttta
     6121 cttattgagg tgaataatga gactgatccc cctgactacc gctgaacagg tcggcaaatg
     6181 ggctgctcgc catatcgtca atcgtatcaa tgcgttcaaa ccgactgccg atcgtccgtt
     6241 tgtactgggc ctgccgactg gcggcacgcc gatgaccacc tataaagcgt tagtcgaaat
     6301 gcataaagca ggccaggtca gctttaagca cgttgtcacc ttcaacatgg acgaatatgt
     6361 cggtctgccg aaagagcatc cggaaagcta ctacagcttt atgcaccgta atttcttcga
     6421 tcacgttgat attccagcag aaaatatcaa ccttctcaac ggcaacgccc cggatatcga
     6481 cgccgaatgc cgccagtatg aagaaaaaat ccgttcttac ggaaaaattc atctgtttat
     6541 gggcggtgta ggtaacgacg gtcatattgc atttaacgaa ccggcgtctt ctctggcttc
     6601 tcgtactcgt atcaaaaccc tgactcatga cactcgcgtc gcgaactctc gtttctttga
     6661 taacgatgtt aatcaggtgc caaaatatgc cctgactgtc ggtgttggta cattgctgga
     6721 tgccgaagaa gtgatgattc tggtgctggg tagtcagaaa gcactggcac tgcaggccgc
     6781 cgttgaaggt tgcgtgaacc acatgtggac catcagttgt ctgcaactgc atccaaaagc
     6841 gatcatggtg tgcgatgaac cttccaccat ggagctgaaa gttaagactt taagatattt
     6901 caatgaatta gaagcagaaa atatcaaggg tctgtaattg ttatccctgc cctggctcct
     6961 tgctcagggc aatatttttt aaaatcgggg gtcagaatgt atgcattaac ccagggccgg
     7021 atctttaccg gccacgaatt tcttgatgac cacgcggttg ttatcgctga tggcctgatt
     7081 aaaagcgtct gtccggtagc ggaactgccg ccagagatcg aacaacgttc actgaacggg
     7141 gccattctct cccccggttt tatcgatgtg cagttaaacg gctgcggcgg cgtacagttt
     7201 aacgacaccg ctgaagcggt cagcgtggaa acgctggaaa tcatgcagaa agccaatgag
     7261 aaatcaggct gtactaacta tctgccgacg cttatcacca ccagcgatga gctgatgaaa
     7321 cagggcgtgc gcgttatgcg cgagtacctg gcaaaacatc cgaaccaggc gttaggtctg
     7381 catctggaag gtccgtggct gaatctggta aaaaaaggca cccataatcc gaattttgtg
     7441 cgtaagcctg atgccgcgct ggtcgatttc ctgtgtgaga acgccgacgt gattaccaaa
     7501 gtgactctgg caccggaaat ggttccagcg gaagtcatca gcaaactggc aaatgccggg
     7561 attgtggttt ctgctggtca ctccaacgcg acgttgaaag aagcgaaagc cggtttccgc
     7621 gcggggatta cctttgccac ccatctgtac aacgcgatgc cgtatattac cggtcgtgaa
     7681 ccgggcctgg cgggcgcgat cctcgacgaa gctgacattt attgcggtat tatcgctgat
     7741 ggcctgcatg ttgattacgc caacattcgt aacgctaaac gtctgaaagg cgacaaattg
     7801 tgtctggtta ccgatgccac cgcgccagca ggtgccaaca ttgaacagtt catttttgcg
     7861 ggtaaaacaa tatactaccg taacggactt tgtgtggatg agaacggtac gttaagcggt
     7921 tcatccttaa ccatgattga aggcgtgcgt aatctggtcg aacattgtgg tatcgcactg
     7981 gatgaagtgc tgcgtatggc gacgctctat ccggcacgtg cgattggcgt tgagaaacgt
     8041 ctcggcacgc tcgccgcagg taaagtagcc aacctgactg cattcacacc tgattttaaa
     8101 atcaccagga ccatcgttaa cggtaacgag gtcgtaactc aataagagaa agtatgacac
     8161 caggcggaca agctcagata ggtaatgttg atctcgtaaa acagcttaac agcgcggcgg
     8221 tttatcgcct gattgaccag tacgggccaa tctcgcggat tcagattgcc gagcaaagtc
     8281 agcttgcccc cgccagcgta accaaaatta cgcgtcagct tatcgaacgc gggctgatca
     8341 aagaagttga tcagcaggcc tccaccgggg gccgccgcgc tatctccatc gtcaccgaaa
     8401 cccgcaattt ccacgcaatc ggcgtacggc ttggtcgtca cgacgccacc atcaccttgt
     8461 ttgatctcag cagcaaagtg ctggcagaag aacattaccc gctgccggaa cgtacccagc
     8521 agacgctgga acatgccctg ctgaatgcca ttgctcagtt tattgatagc taccagcgca
     8581 agctacgcga gctgatcgcg atttcggtga tcctgccagg gcttgtagac ccggacagcg
     8641 gcaaaattca ttacatgccg catattcagg tagaaaactg ggggctggtt gaagcactgg
     8701 aagagcgttt taaagtgacc tgttttgtcg gccacgatat ccgcagtctg gcgctggcag
     8761 agcactactt cggtgcaagt caggattgcg aagactccat tctggtgcgt gtccatcgcg
     8821 gaaccggggc cgggattatc tctaacgggc gaatttttat cgggcgcaac ggcaacgtcg
     8881 gtgaaattgg ccatattcag gtcgaaccgc tgggtgaacg ctgccactgc ggcaactttg
     8941 gctgcctgga aactatcgcc gccaatgctg ccattgaaca acgggtgttg aatctgttaa
     9001 agcagggcta ccagagccgt gtgccactgg acgactgcac catcaaaact atctgcaaag
     9061 ccgcgaacaa aggcgatagc ctggcctcgg aagtgattga atatgtcggt cgtcatctgg
     9121 gtaaaaccat cgccattgct atcaacttat ttaatccgca aaaaattgtt attgctggag
     9181 aaatcaccga agccgataaa gtgctgctcc ctgctattga aagctgcatt aatacccagg
     9241 cgctgaaggc gtttcgtact aatctaccgg tggtacgttc tgagttggat caccgctcgg
     9301 caatcggcgc ttttgcgctg gtaaaacgcg ccatgctcaa cggtattttg ctccagcatt
     9361 tgctggaaaa ttaatgtgct tttatagtgg cgcttattgt tgtcaatatt ctgggtagtc
     9421 catgaccatt aaaaatgtaa tttgcgatat cgacggcgtg ctgatgcacg ataacgtcgc
     9481 cgtaccgggt gcagcggaat ttttgcacgg gattatggat aaaggcctgc cgctggtgtt
     9541 gctgaccaac tatccttcgc agaccgggca agatctggcg aatcgctttg ccactgccgg
     9601 tgtcgatgta ccggatagcg tgttttatac ctccgcaatg gcgaccgccg attttctgcg
     9661 tcgccaggaa ggcaagaaag cgtatgtggt gggcgaaggc gcactgattc atgaactgta
     9721 caaagccggt ttcactatta ccgatgtgaa ccctgatttt gtgattgttg gcgaaacgcg
     9781 ttcctacaac tgggacatga tgcataaagc agcctatttc gtcgctaacg gtgcacgctt
     9841 tatcgccacc aacccggaca cccacgggcg cggtttttat cccgcctgtg gcgcgttgtg
     9901 tgcagggatc gagaaaatct ccgggcgcaa accgttctat gttggaaagc cgagtccgtg
     9961 gatcatccgc gcggcattaa acaaaatgca ggcgcattcg gaagaaacgg tgattgtcgg
    10021 cgataacttg cgtaccgata ttctggcggg cttccaggcc ggtctggaga cgattctggt
    10081 gctttctggt gtttcgtcgc tcgacgatat cgacagtatg ccgttccgcc caagttggat
    10141 ttacccatcg gtcgctgaaa tcgacgttat ctgaatagca accacgcctc agggcgtggt
    10201 ttcttatttt tacccatcat taaaatcacc ccattcaata aaacccgtca aaaactatcg
    10261 atccatcaat aaatataccc aaaagccctc ccctttttca tcaatcagca atcgccatta
    10321 cgttttttat ttttccactg ccaaatcgct tgcgcaccgt gctgctttgc ggcatttttt
    10381 taacaagtaa acacaacacg cagtaaacac caggttaatg gagaaggtta tgtgttcaat
    10441 ttttggcgta ttcgatatca aaacagacgc agttgagctg cgtaagaaag cactcgagct
    10501 gtcacgcctg atgcgtcatc gtggcccgga ctggtccggt atttatgcca gcgataacgc
    10561 cattctcgcc cacgaacgtc tgtcaattgt tgacgttaac gcgggggcgc aacctctcta
    10621 caaccaacaa aaaactcatg tgctggcagt aaacggtgaa atctacaacc accaggcatt
    10681 gcgcgctgaa tatggcgatc gttaccagtt ccagaccggg tctgactgtg aagtgatcct
    10741 cgcgctgtat caggaaaaag ggccggaatt tctcgacgac ttgcagggca tgtttgcctt
    10801 tgccctgtac gacagcgaaa aagatgcata tctgattggt cgcgaccatc tggggatcat
    10861 cccactgtat atgggctatg acgaacacgg tcagctgtat gtggcctcag aaatgaaagc
    10921 cctcgtgcca gtttgccgca cgattaaaga gtttccggcg gggagctatt tgtggagtca
    10981 ggacggcgaa atccgttctt attatcatcg cgactggttc gactacgatg cggtgaaaga
    11041 taacgtgacc gacaaaaacg agctgcgtca ggcactggaa gattccgtta aaagccatct
    11101 gatgtctgat gtgccttacg gtgtgctgct ttctggtggt ctggattcct caattatttc
    11161 cgctatcacc aagaaatacg ccgcccgtcg cgtggaagat caggaacgct ctgaagcctg
    11221 gtggccgcag ttgcactcct ttgctgtagg tctgccgggt tcaccggatc tgaaagcagc
    11281 ccaggaagtg gcaaaccatc tgggcacggt gcatcacgaa attcacttca ctgtacagga
    11341 aggtctggat gccatccgcg acgtgattta ccacatcgaa acttatgatg tgaccactat
    11401 tcgcgcttca acaccgatgt atttaatgtc gcgtaagatc aaggcgatgg gcattaaaat
    11461 ggtgctgtcc ggtgaaggtt ctgacgaagt gtttggcggt tatctctact tccacaaagc
    11521 gccgaacgct aaagagttgc atgaagagac ggtgcgtaaa ctgctggccc tgcatatgta
    11581 tgactgcgcg cgtgccaaca aagcgatgtc agcctggggc gtggaagcac gcgttccgtt
    11641 cctcgacaaa aaattccttg atgtggcgat gcgcattaac ccacaggata aaatgtgcgg
    11701 taacggcaaa atggaaaaac acatcctgcg tgaatgtttt gaagcgtatc tgcctgcaag
    11761 cgtggcctgg cggcaaaaag agcagttctc cgatggcgtc ggttacagtt ggatcgacac
    11821 cctgaaagaa gtggcagcgc agcaggtttc tgatcaacag ctggaaactg cccgcttccg
    11881 cttcccgtac aacacgccga cctcaaaaga agcgtatctg taccgggaga tctttgaaga
    11941 attgttcccg cttccgagcg ccgctgagtg cgtgccgggc ggtccttccg tcgcttgttc
    12001 ttccgctaaa gcgattgagt gggatgaagc gttcaagaaa atggacgatc cgtctggtcg
    12061 cgcggttggc gttcaccagt cggcatataa ataagacgtg taatattgcc ccggagaaat
    12121 ccggggcaat ttcagttact ggggcgcttg ttgattctga tatgcagtaa ggaaatgttc
    12181 cgggtttgcc acatcgctat ttgacaactc atcactgtag gccaccaacc aggtcgcctg
    12241 ctcatccgga atatgcagtc tgccatttcc ctctttcagg cgtaataact caaaagattt
    12301 gcccttaatt acggcggtat aggatttctc accttcctgt aatgctttca ctttacagcg
    12361 aaatatctga ccatcgatgt cattatcaac atgcgatacc tgaatgaggg cttcattaga
    12421 agctttcggg cctatccgca tcatccacac cacagcgcct tcctgcccac gatagccaaa
    12481 aacatattta ccgatttccg gtttaaccgt atcggcgaac gcagattgca taagtaataa
    12541 cgcacccatc aacgccgtca taaaacgcag catagtattc cctcaataaa tcgcctgaag
    12601 tggtagcaga gatgcaggcg tagcgatccc tgccctgtcc tgttgcaacg ggccagtatt
    12661 tcatagatta atttcccacc actattgttg aattccgaat ttcatcctgt atgacaaaag
    12721 aatggctgta gggtggggaa aaaacacaaa gtaaacaata attgacgaat atagcgccat
    12781 gctgttcgca acctaaccaa acagtcactt tcgagcaatt ttccttgaaa aagaggttga
    12841 cgctgcaagg ctctatacgc ataatgcgcc ccgcaacgcc gataaggtat cgcgaaaaaa
    12901 aagatggcta cgtagctcag ttggttagag cacatcactc ataatgatgg ggtcacaggt
    12961 tcgaatcccg tcgtagccac catctttttt ttgcgggagt ggcgaaattg gtagacgcac
    13021 cagatttagg ttctggcgcc gcaaggtgtg cgagttcaag tctcgcctcc cgcaccattc
    13081 accagaaagc gttgtacgga tggggtatcg ccaagcggta aggcaccggt ttttgatacc
    13141 ggcattccct ggttcgaatc caggtacccc agccatattc ttcgagtaag cggttcaccg
    13201 cccggttatt ggggtatcgc caagcggtaa ggcaccggtt tttgataccg gcattccctg
    13261 gttcgaatcc aggtacccca gccatcgaag aaacaatctg gctacgtagc tcagttggtt
    13321 agagcacatc actcataatg atggggtcac aggttcgaat cccgtcgtag ccaccaaatt
    13381 ctgaatgtgt cgaatatgtt cggcaaattc aaaaaccaat ttgttggggt atcgccaagc
    13441 ggtaaggcac cggattctga ttccggcatt ccgaggttcg aatcctcgta ccccagccac
    13501 attaaaaaag ctcgcttcgg cgagcttttt gcttttctgc gtatattcaa tgccgaatac
    13561 gatgttgact cgtcttatcc ttacaaagtc ggatgcgacg ctgccgcgtc ttatccgacc
    13621 tacggttggc atgcatccag caatgttgtg aggctacaac cctaacgcat atttcagcgc
    13681 cttacgtttc aacacgccag cacgctccgc cgccatcaaa ccaagattac gcacaaaacg
    13741 cagcggtggc agattattgc tgaatccggc ataaaacaga tccataccac tttgcatcat
    13801 gaagttatcc gccatacgcc gcatctggta acgcttgagc acaggataac tggcccacgc
    13861 ttcgccgtag ctgcgggcgt tcaccagaac gtcaatcagg gcatcgacat cacgataacc
    13921 aagattcact ccctgccccg ccagcggatg gatggtatgc gcggcatcgc ccaccagagc
    13981 aagccctggt tgcacatact gcaacgcatg gcgacgtata agcggaaacg caccagcggc
    14041 aagcggtgtc acgtaaccca gacgcgacgg gaaatgcttc gcgatttccg cctgtagctg
    14101 cgccatattc atattctgca actggcgaat gcgcgccgga gagtcatacc acaccagcga
    14161 tgcccagtta tcaaacaacg gcagaaacgc acgcggtccg tccggggtaa attgctgcca
    14221 ggtgctgtcg ccgggatcgt tctcacactg tacgctaatc aacatacacg actgcgcata
    14281 ctgccaggca tgaacgccaa ttcccgccat ctgtcgcacc tgcgaatttg cgccgtcggc
    14341 accaatcacc agcttcgcga ggatcgtttc accgcccttc agttccagct cctgaagatc
    14401 attatggcga tgcagtgcag tcagcgaggt tggcacacgt aacgttactt tcggatgcgc
    14461 ttccagcgcc tgccacaaag cctgttgcag gacagtgttt tccaccatgt agccaagcaa
    14521 cggtagctta agttcagcgg cgtcaaacac gacatgcgcc gtttcccact cccacgtttc
    14581 cagtctgcgg taaggatggc aacgcatagc ctgtactgca tcccagaccc ctaacccttt
    14641 aagcaatgat accgaagccg cgctgatcgc cgagatccgc acatccggtt ggctgtcagc
    14701 gacaaacggc gcaggttctg cgtgctcgat caccgttacc gtaaacccgt gctgtgccag
    14761 ccccagcgcc agtgcgccgc cgaccattcc tccgccgaca atggcaattt ccgttggttg
    14821 atttgtcatg gtcaatcatc ctgttagcaa tatgctttaa gtttacagga tttttacccc
    14881 caagggctga taacgctcat actggtcaca acggcagcaa agcattacac tatgcgccct
    14941 gcattcctgg ctactatttc gcaagagcaa gtcgatgacc aaaaaactcc atattaaaac
    15001 ctggggctgt cagatgaacg agtacgattc atcgaagatg gccgatctgc tggatgccac
    15061 ccacggctat caactgaccg acgtggcgga agaagcggat gtgctgctgc tgaacacctg
    15121 ctcaatccgc gagaaggctc aggaaaaagt cttccatcag ttgggtcgct ggaaactgtt
    15181 aaaagagaag aatccagacc tgattatcgg cgtcggcggc tgcgtggcat cgcaagaagg
    15241 cgagcacatt cgccagcgcg cccactatgt cgatattatt tttgggccgc aaacgctgca
    15301 tcgtctaccg gagatgatca actccgtgcg cggcgaccgc agcccggttg tagatatcag
    15361 cttcccggaa atcgagaagt ttgaccgtct gccggaaccg cgcgccgaag ggccgaccgc
    15421 gtttgtctcc atcatggaag gctgcaataa atattgcacc tactgcgtgg tgccttacac
    15481 ccgtggtgaa gaggtaagcc gtccgtccga cgatattctg tttgagatcg cccagcttgc
    15541 ggctcagggc gtgcgtgaag tcaacctgct cggtcagaat gtgaatgcct ggcgtggtga
    15601 gaactacgac ggcaccaccg gaacgtttgc cgatctgctg cgtctggttg cggcgattga
    15661 cgggatcgat cgtattcgct ttaccaccag ccatccgatc gaattcaccg acgatatcat
    15721 cgaagtgtat cgcgacacgc cggagctggt gagcttcctg cacttgccgg tacagagcgg
    15781 ttccgatcgc attctgaacc tgatgggccg tactcacacg gcgctggagt acaaagcgat
    15841 catccgtaaa ctgcgtgcgg cgcgtccgga tattcagatc agttctgact tcatcgttgg
    15901 cttccctggc gaaaccaccg acgacttcga gaaaacgatg aagctgattg ccgacgtcaa
    15961 tttcgacatg agctacagct ttatcttctc cgctcgtcca ggaacgcctg ccgccgatat
    16021 ggttgatgat gttccggaag aagagaagaa gcagcgtctg tatattctgc aagagcgcat
    16081 taatcagcaa gcgatggcat ggagccgccg tatgctcggc accacccagc gtattctggt
    16141 agaaggtaca tcgcgtaaga gcatcatgga gctttccggt cgtactgaaa ataaccgcgt
    16201 ggttaacttc gaaggcacgc cggatatgat cggtaaattc gtcgatgtag aaattaccga
    16261 cgtctatcca aactccctgc gcggaaaagt ggtgcgtact gaagatgaaa tgggtctgcg
    16321 cgtcgcagaa acaccggaat cggtgattgc ccgtacccgc aaagaaaacg accttggcgt
    16381 gggttattat cagccgtaat tctcaggccc gccgttccgg tgggccttgt tttttctctc
    16441 tttatcccca tctttatcgc aatgcttgcg cctttttcct gtggcgtgaa taatttcctt
    16501 ataggccggt gaatgttcag cttccggcgg catacagata agaggaacgg tttgaacata
    16561 gacactcgcg aaatcaccct ggagccagca gacaatgcgc gtctgttgag cctgtgcggc
    16621 ccgtttgatg acaacatcaa acaactcgaa cgccgtctcg gcatcgaaat caatcgccgt
    16681 gataaccact ttaaactgac cggccgtccg atttgcgtca ccgctgcggc agacattctg
    16741 cgtagcctgt atgtcgatac tgccccgatg cgcggtcaga ttcaggatat cgaaccggaa
    16801 cagatccacc ttgcgattaa agaagcgcgg gtactggagc aaagcgcgga gagcgtgccg
    16861 gagtacggca aagcagtcaa tatcaaaacc aaacgcggcg taattaagcc gcgcacgcca
    16921 aaccaggcgc agtacatcgc caatattctc gaccatgaca ttaccttcgg cgttggcccg
    16981 gcgggtacgg gtaaaaccta cctggcagtg gctgcggcag ttgatgccct ggagcgtcag
    17041 gaaattcgcc gtattctgct gactcgtccg gcggtcgaag ccggtgagaa actgggcttc
    17101 ctgcctggcg atttaagcca gaaagtagac ccgtatctgc gcccgctgta cgacgcgctg
    17161 tttgaaatgc tgggctttga gaaagtcgag aaactgattg agcgcaacgt tattgaagtc
    17221 gcaccgctgg cctatatgcg tggtcgtacg ctgaacgacg cgtttatcat tcttgatgag
    17281 agccagaaca ccaccatcga acagatgaag atgttcctga cccgtatcgg ttttaactca
    17341 aaagcggtta tcaccggcga cgtcacacag atcgacctgc cgcgtaatac caagtctggt
    17401 ttgcgtcatg ccatcgaagt gctggctgat gtcgaagaga tcagctttaa cttcttccac
    17461 agcgaagacg tggttcgtca cccggtggtg gcgcgtatcg ttaacgctta tgaagcctgg
    17521 gaagaagccg aacaaaaacg taaagcggcg ctggcggcag aacgcaagcg cgaagaacag
    17581 gaacaaaaat gagtcaggtg atcctcgatt tacaactggc atgtgaagat aattccgggt
    17641 taccggaaga gagccagttt cagacatggc tgaatgcggt gatcccgcag tttcaggaag
    17701 aatcggaagt gactattcgc gtggtcgata ccgccgaaag ccacagtctg aatctgacct
    17761 atcgcggtaa ggataagccg accaacgtgc tctccttccc gtttgaagtg ccgcctggca
    17821 tggaaatgtc gctactgggc gatctggtta tctgccgtca ggtggttgag aaggaagctc
    17881 aggagcaagg caaaccactg gaggcgcact gggcgcatat ggtggtgcac ggcagtctgc
    17941 atttgttagg ttacgatcac atcgaagatg acgaagcaga agaaatggaa gccatcgaaa
    18001 cagagattat gcttgctctg ggctatgagg atccgtacat tgccgagaaa gaataatttg
    18061 ccaggcattt gactggcaga cgtccccgcc gttggtgatt aacgacggcg ttttgattaa
    18121 ctaacatgag aacccatacg acgccatgag cgacgacaat tcacacagta gtgacacgat
    18181 aagcaacaag aagggatttt tctccctgtt actcagccaa cttttccacg gtgaaccgaa
    18241 aaaccgtgac gaactgctgg cgctgatccg tgattccggg cagaacgacc ttatcgacga
    18301 agatacgcgc gatatgctcg aaggggtgat ggacatcgca gaccaacgcg ttcgcgacat
    18361 catgatcccc cgctcccaga tgattaccct gaaacgcaac cagacgctgg acgaatgcct
    18421 tgatgtcatc atcgagtccg cccactcacg tttcccggtg attagcgaag acaaagatca
    18481 cattgaaggg attctgatgg cgaaagactt gctgccgttt atgcgcagcg atgctgaagc
    18541 cttcagcatg gacaaagtgt tacgtcaggc ggttgtcgtt cctgaaagta agcgcgtaga
    18601 ccggatgctg aaagagtttc gctctcagcg ttaccacatg gcgatcgtca ttgacgaatt
    18661 cggtggggtt tccggtctgg tgaccattga agacatcctg gaactgattg ttggtgagat
    18721 tgaagacgag tatgacgaag aagatgatat cgacttccgt cagctgagtc gtcatacctg
    18781 gaccgtgcgc gcactggctt ctattgaaga cttcaacgaa gcgttcggca cccactttag
    18841 cgatgaagag gttgacacta tcggtggtct ggtgatgcag gcatttgggc atcttccggc
    18901 gcgtggcgaa accatcgaca tcgacggtta ccagttcaaa gtggcgatgg ccgacagtcg
    18961 gcgtattatt caggttcatg tcaaaatccc ggatgactca ccccagccga agctggatga
    19021 ataaaaccga aactggatag ataactacat ggcttttgcc tcattaattg aacgccagcg
    19081 cattcgcctg ctgctggcgt tattattcgg tgcctgcgga acgctggcct tctctcctta
    19141 cgacgtctgg cctgcggcga ttatttcgct gatggggctt caggcgttga cctttaaccg
    19201 ccgtccactc cagtctgccg ctattggctt ttgctgggga tttggcctct ttggcagcgg
    19261 tattaactgg gtctatgtca gcatcgcgac ctttggcgga atgcctggcc cggttaacat
    19321 cttcctggtg gtactgctgg cggcgtattt gtcgctgtat accggactgt ttgctggcgt
    19381 gctgtcgcgt ctgtggccga aaaccacctg gctgcgcgtg gcgattgccg cccctgctct
    19441 ctggcaagtg accgagtttc tgcgcggttg ggtactaaca ggcttcccgt ggttacagtt
    19501 cggctatagc cagattgatg gcccgttaaa agggctggca ccgataatgg gcgtggaagc
    19561 cattaacttc ctgctgatga tggttagtgg cctgctggca ctggcgttgg tcaaacgcaa
    19621 ctggcgtccg ctggtggtgg ccgtcgtgct gtttgccctt cccttcccgc tgcgttacgt
    19681 ccagtggttt accccgcaac cggagaaaac cattcaggtt tcgatggttc agggcaatat
    19741 tccgcaatcg ctgaaatggg acgaaggcca gcttcttaat acgctgaaga tttactacaa
    19801 cgcaacggca ccgctgatgg gcaaatcatc gctgattatc tggccggagt cggcgataac
    19861 cgatctggaa attaatcagc aaccgttcct caaagctctg gacggtgagt tgcgcgataa
    19921 aggtagctcg ctgatcaccg ggattgtcga cgcgcgtctc aataaacaga accgctacga
    19981 tacctacaac accatcatca cactgggtaa aggcgcatcg tacagctatg aatcagccga
    20041 tcgctataac aaaaaccatc tggtgccgtt tggcgagttt gtcccactgg agtcgattct
    20101 gcgtccatta gcaccgttct ttgatttgcc gatgtcgtca ttcagccgcg gaccatatat
    20161 tcagccaccg ctgtcgttga atggcattca acttactgcg gccatttgct acgaaatcat
    20221 tctcggcgag caagtgcgcg ataacttccg cccggatacc gactatctgc tgactatctc
    20281 caacgatgcg tggtttggta agtctattgg tccatggcag cacttccaga tggcgcgaat
    20341 gcgtgcgctg gagctggcgc gcccactgtt gcgcagcacc aacaacggca ttacggcggt
    20401 gattggcccg cagggtgaga ttcaggcgat gatcccgcag ttcacccgcg aggtgttgac
    20461 cactaacgtg acaccaacca ccggactcac accgtacgca cgaaccggca actggccgct
    20521 gtgggtgctg acggcgctgt ttggttttgc cgctgtactg atgagtctgc gtgctcgcaa
    20581 acgttaatgt gcctgatgcg cgacgcttat caggcctacg cgatttttgc aatatgttga
    20641 attttcgcga ttttgtagga taaagcgtac tttttcaaca cgctctgggg catgattatc
    20701 atgccctttt ttcttacggt tcagggatac aagccacatc gccatattgg ggatgccgaa
    20761 taattgcctg cggatcaaat gaactttctc tgtccagttg tattagcccg ttcttcgcca
    20821 taacaaatat ctttctacca tccgctgaaa aacgaagatg attcacgtag ctggcaggat
    20881 aaacgccaat aatctgcccg gcattaatat tgagcaggat gacataacct tgcaggccat
    20941 atgacaccaa cgctaaatac tgaccgtccg ggctaatacc ctcaacggaa taaacgctcc
    21001 tgctacacca ttgcgtcata atgctggctt gttgatcctc agctcttata cgtgaaatcc
    21061 cctggctatc ggcaaaaata atctcaccat tttttggtcg gaatcccatc gcagaaacga
    21121 caccacgcgc atcacctgcg ggaaagtctt ctttctgatg cgtgatggta ttgatccgca
    21181 cgatgttacc tctgcctgct cccggaacca gtaatgtttt ttcattcggg gaaagagcca
    21241 taacccaggc gcctggttta ccgctcatcc ccgtcccgtc gtatttctcc agcgggatat
    21301 ccaccacatg ctgataagcc accagatcaa tcgctgacac actattgctg gtgcgattca
    21361 tcacccaggc ttttgtgcca tcacgcgaga ggataaggct gtcaggatgt gcgccggtgg
    21421 taatcgactg aataatctcc cccgtcgcca cgttaatgac cgataacgca ttcgcttcaa
    21481 atgaagtcgc ataaagcaac gtttcatccg ggcttagcgc cagcgacctg acgccctgat
    21541 ctttacagcc accacaatgt ttgactgact tttctttcgc tatgggcgct gtaaaggtat
    21601 gcaggatttt cttttgtccg aggtcgaaaa caacaatgct gttcgtgttt tcaattgcgg
    21661 catacaacgt attagcatcc cgactgatcg ccatatcatg gatatcggca atctcagcga
    21721 gtaaatcacc actggcctgc tcacgactta ccggagcgga taatgtttcc ctgagggcgt
    21781 tgcgctcctc cactaactgc tgcttttttt gtcgtcgctc ttgttcaatc tcctgcggat
    21841 aaaacgcttc acgcaggctt tgcctgacct gtaaaactga ttcatcaatc tgctgctgca
    21901 caagccagat agtcccggtc agaagaagag tgaccgcaac aataatcccg ccttccgtaa
    21961 cgagccgatt ttgcccaact aaacgcgcac tcagccaacc caacgcgccc cccgcaatca
    22021 ggccgccaat gtggcaagcg ttatctactc ctgtttgccg ggtaccatac aacagtgtca
    22081 gcgccaccat acccaacaga ggaaatactc gccgctggtt tttatggtgt gtgcccgcac
    22141 ctgaagcaag ctgtgttgct atcgcagcgc ccgctattcc cataattgcc ccggatgccc
    22201 caacgctgat gactatctgc tcactttcgc gtaacgcata acaggcgctg atgagtccac
    22261 caaagacgcc ggagaataac cagataatca ggagtcgcca tttccccagt atccgttccg
    22321 ccactgcccc cactaccagg agtgcaaaca tattcatcag caaatgggaa aaactactgt
    22381 gcagaaaaac actgctcaat aagcgccagg attcatccga caacgtgaga gaagcaacgt
    22441 tagcgccgag atggatgagt gcggattgat cgacttcttc gtaactcatc cattgtgcga
    22501 ggtaacaaaa tatcccaaca cagagcgcaa aaaggatcag ggttattgcg ggtaattgaa
    22561 catttaacgg cttaaccgaa gatgcagaca taaggtgatt cttccttgaa aagactagta
    22621 aaaagcattt tcccgtgtgt agttatcatc ccggcaaacg tcccacaccg aaaagttaac
    22681 actgtccgaa atatgacttt ttcaaactga aattacaatc gtaattaatt cacccctaag
    22741 tattttttgg catgtaagtt gctttattta ctgtgtaagc aatgaaatca gccgcaacgc
    22801 aaccgggttg caccaccaca aatcagcggt agcaccaaaa aggtgcggtt taagaatttt
    22861 gcttcggaat ggtgcggcgc acctcgcaaa aataaacaat aaaacagcaa tatctttaca
    22921 tttatccaaa ctaaatgtta cctaacaaac acaacactgc acaataaagt cgcagatgat
    22981 aacaacacaa acactcacaa cgggtatcca tgcgttttta acgcagaaga taaaggagtt
    23041 ggatatgcaa ttacgtaaac ctgccacagc aatcctggcc ctggcgcttt ccgcaggact
    23101 ggcacaggca gatgacgccg ccccggcagc tggcaatacg ctggacaaaa tcgccaaaaa
    23161 cggcgtgatt gtcgtcggtc accgtgaatc ttcagtacct ttctcttatt acgacaatca
    23221 gcaaaaagtg gtgggctact cgcaggatta ctccaacgcc attgttgaag cagtgaaaaa
    23281 gaaactcaac aaaccagact tacaggtgaa attgattccg atcacctcac aaaaccgtat
    23341 tccactgctg caaaacggca ctttcgattt tgaatgcggt tctaccacca acaatgccga
    23401 acgccaaaaa caggcggctt tctctaacac tattttcgtg gtcggtacgc gcctgctgac
    23461 caaaaagggc ggcgatatca aagattttgc cgacctgaaa ggcaaagccg tagtcgtcac
    23521 ttccggcacc acctctgaag ttttgctcaa caaactgaat gaagagcaaa aaatgaatat
    23581 gcgcatcatc agcgccaaag atcacggtga ctctttccgt accctggaaa gcggtcgtgc
    23641 cgttgccttt atgatggatg atgctctgct ggccggtgaa cgtgcgaaag cgaagaaacc
    23701 agacaactgg gaaatcgtcg gcaagccgca gtctcaggaa gcctatggtt gcatgttgcg
    23761 taaagacgat ccgcagttca aaaagctgat ggatgatacc atcgctcagg tgcagacctc
    23821 cggtgaagcg gaaaaatggt ttgataagtg gttcaaaaat ccaattccgc cgaaaaacct
    23881 gaacatgaat ttcgaactgt cagacgaaat gaaagcactg ttcaaagaac cgaatgacaa
    23941 ggcactgaac taattacaag aaccaggggc ggaaattcca gccctctcga ttgttacgta
    24001 gcacggacag actatacgcc tggtggtcgt tccccatcgg gcctgaaaac cgcaatacgc
    24061 tgggtaacaa tcttcgaggg tagcagttaa cgctgctacc ctcttttttc tggagtagat
    24121 ttatgtctat agactggaac tggggtattt ttttacaaca agccccgttc ggcaacacca
    24181 cctatcttgg ttggatctgg agtggctttc aggtgacgat cgctttgtcg atctgcgcct
    24241 ggattatcgc tttcctcgtc ggttcatttt tcggcatttt acgtaccgtt ccaaaccgtt
    24301 ttctctccgg tctgggtacg ttgtatgtcg aactgttccg caacgtgccg ttgattgtgc
    24361 aattctttac ctggtaccta gtgatcccgg agctgctgcc agagaaaatc ggcatgtggt
    24421 ttaaggcaga gctggatccc aatattcagt ttttcctttc atccatgctc tgcctggggc
    24481 tgtttaccgc cgcccgtgtt tgcgaacagg tacgcgcagc tattcagtcg ctgccgcgtg
    24541 ggcaaaaaaa tgccgcgctg gcgatgggtc tgacgctacc gcaggcttac cgctacgtac
    24601 tgctgcctaa tgcttatcgc gttatcgtcc cgccgatgac ctcagagatg atgaacctgg
    24661 tgaaaaactc cgccatcgcc tcgactatcg gtctggtgga tatggcggcg caggcgggta
    24721 aattgctcga ttactcggcc cacgcctggg agtcgtttac cgccatcacg ctggcgtatg
    24781 tattgattaa cgcttttatc atgctggtga tgacgctggt tgaacgtaaa gttcgcctgc
    24841 ccggcaacat ggggggcaaa taatgtacga gtttgactgg agttccattg ttccttccct
    24901 gccatatctg ctcgacgggc tggtgatcac gctgaaaatc accgtcacgg cagtggtgat
    24961 tggtattttg tggggcacca tgctggcggt gatgcgcttg tccagctttg cgcccgtagc
    25021 gtggtttgcc aaagcctacg ttaacgtatt ccgctcgatt cctttagtta tggttttgct
    25081 gtggttttac ctgatcgtac cgggttttct gcaaaacgtg ctgggattat cacccaaaaa
    25141 tgatatccgt ttaatttcgg cgatggtggc gttttcgatg tttgaagcgg cctactattc
    25201 agagattatc cgtgctggta ttcaaagtat ttctcgtggt cagtcgagcg ccgcgctggc
    25261 gttgggaatg actcactggc agtcgatgaa actgattatt ctgccgcagg cgttccgcgc
    25321 gatggtgccg ctgttgctca ctcagggcat cgtactgttt caggatacct cactggtgta
    25381 tgtgttatct ctggccgatt tcttccgtac cgcctcaacc attggtgagc gtgatggtac
    25441 gcaggttgag atgatcctgt ttgccggatt tgtttatttc gttattagcc ttagcgcgtc
    25501 gttgttggtc agctacttga aaagaaggac agcataatga ttaccctgaa aaatgtttca
    25561 aaatggtatg gtcactttca ggtgctgacc gactgctcaa ccgaagtgaa aaaaggcgaa
    25621 gttgtggtgg tttgcggccc gtctggttcc ggcaaatcaa cgctgattaa aaccgtcaac
    25681 ggcctcgaac cggtgcagca aggtgaaatc accgtcgatg gcatcgtggt taacgacaag
    25741 aaaaccgatc tggcgaaact gcgttcccgc gtcgggatgg tgtttcagca tttcgagctg
    25801 ttcccccatc tgtcgattat cgaaaacctg accctggcgc aggtgaaagt gcttaaacgc
    25861 aataaagcgc cggctcgtga aaaagcattg aaattgctgg agcgcgtcgg gctttccgca
    25921 catgccaata agtttccggc gcagctttcc ggcggccagc agcagcgtgt ggcgatcgct
    25981 cgcgcattgt gcatggatcc tattgccatg ctgttcgacg aaccaacgtc ggcgctggat
    26041 ccggagatga tcaacgaagt gctggacgtg atggtcgaac tggcgaacga aggaatgacg
    26101 atgatggtgg tgacccacga aatgggcttt gcccgtaaag tggcgaatcg ggtgatcttt
    26161 atggacgagg gtaaaattat cgaagactcg ccgaaagacg ctttcttcga taatccgaaa
    26221 tcggaccgcg caaaagactt cctcgcaaaa atcctgcatt aatcactctg gcgcgcatac
    26281 tcaattgcgc gccatctcac gctttctcgt ttcgtccctc gtatctgtta catcccggcg
    26341 ttacccttgt cgcaaagaaa cacacaacaa ggagcaacaa tggcactgcc aattctgtta
    26401 gattgcgacc caggtcatga cgacgctatc gcaatagttc tcgccctcgc ctcaccagag
    26461 cttgatgtca aagcaattac gtcttccgcc ggaaaccaga caccagaaaa aaccttacgc
    26521 aatgttctgc gtatgctgac cttgcttaat cgcaccgata ttccggtagc aagcggtgcg
    26581 gtaaaaccgt taatgcgtaa tttgattatt gcggacaacg tgcacggcga aagcggcctg
    26641 gacggcccgg cattgccgga accggcattt gcaccgcaaa actgcacggc agtggagctg
    26701 atggcgaaaa cgctgcgtga aagtgcggaa cctgtcacca ttgtttctac cggaccgcaa
    26761 actaacgttg ccttgctgct caatagccac ccggaactgc atagcaaaat tgcccgtatc
    26821 gtgattatgg gcggcgcaat ggggcttggt aactggacgc ctgcggctga atttaatatt
    26881 tacgttgacc cggaagcggc agaaattgtc ttccagtcag gaattccggt ggtgatggcc
    26941 ggtctggatg ttactcacaa agcacaaatc cacgttgaag acactgagcg tttccgcgcg
    27001 attggcaacc ctgtttcaac cattgttgcc gaactgctgg atttcttcct cgaatatcac
    27061 aaagacgaaa aatggggctt tgtcggcgca ccactgcatg acccatgcac catcgcctgg
    27121 ctgttgaaac cggagttgtt taccactgtt gagcgctggg ttggcgtgga aacacaaggg
    27181 aaatataccc agggtatgac agttgttgat tattattatc tgactggcaa taaaccgaat
    27241 gccaccgtaa tggtcgatgt tgatcgtcag ggctttgttg atttactggc tgatcgtctg
    27301 aaattttacg cttaacatac gtcattgatt aagatcacca cgcattacct gggtggtctt
    27361 aatctggtta tctctttttc tttcaagata taaatgcaat ggataatgca gaacttgcca
    27421 ttggtatcga tctcggtact accaatagtt taattgccgt ctggaaagac ggtgccgcgc
    27481 aattaattcc aaataagttc ggtgaatatt taacaccatc cataattagc atggatgaaa
    27541 ataatcatat tttagtcgga aaaccggctg tatcacggcg gacttcgcat ccggataaaa
    27601 cggcagcgtt atttaaacgt gcaatgggca gtaataccaa ctggcggtta ggcagcgaca
    27661 catttaacgc gccagaactg tcctctttgg tattacgctc attaaaagaa gatgccgaag
    27721 aatttctgca acgtccgatt aaagatgtgg tgatctccgt tcctgcttat ttcagcgatg
    27781 aacaacgcaa gcatacccgt ttagcagcag agttagccgg gctaaatgca gtgcgcctga
    27841 ttaatgagcc aacggcagcc gcgatggcgt atggcctgca tacgcagcaa aatacccgct
    27901 cgctggtttt tgatctcggt ggcggcacgt tcgacgttac ggtgctcgag tacgccacgc
    27961 cggtgattga agttaacgcc tccgctggcg ataacttcct cggtggcgaa gattttaccc
    28021 atatgctggt cgatgaggtt ttaaaacgcg cggaagttgc caaaaccacg cttaacgata
    28081 gcgaactggc agccttgtac gcctgcgtgg aagcggcaaa atgtagcaat caatcgccaa
    28141 tgcacattcg ctggcagtac caggatgaaa tgcggaaatg cgaattttac gagaacgaac
    28201 tggaagattt gtggttgccg ctgctcaatc gcttgcgagt gccgattgaa caggcgttgc
    28261 gcgatgcacg tctgaagccg agtcaaatcg acagtctggt gctggttggc ggcgcgtcac
    28321 aaatgccgct ggtgcagcga atcgcagtgc gtctgtttgg caaattaccg tatcaaagtt
    28381 acgatccgag caccattgtc gcgctgggcg cagcaatcca ggccgcctgc cgcttacgca
    28441 atgaagatat tgaagaagtg atcctcactg atatttgccc ttactcgttg ggtgttgaag
    28501 ttaaccgtca gggcgtatcc ggcattttct cgccgattat tgaacgaaac accacagtac
    28561 cggtgtcgcg tgtagaaact tattcaacca tgcacccgga acaggattca attacggtta
    28621 acgtatatca gggagaaaac cacaaagtta aaaataacat tcttgtggaa tccttcgatg
    28681 tgccgttgaa gaaaaccggg acttatcagt cgattgatat tcgctttagt tatgatatca
    28741 acggcttgct tgaagttgat gtgcttctgg aagacggcag cgttaagtcc agagtgatta
    28801 atcacagtcc ggtaacattg agcgcgcagc agattgaaga gagtcggacg cggttatccg
    28861 cattgaaaat ttatccgcgc gatatgctca tcaatcgcac ctttaaagcc aaactggaag
    28921 agttgtgggc gcgggcgctg ggtgacgagc gagaagagat tggtcgggtg atcaccgatt
    28981 ttgatgcggc gttgcagtca aacgatatgg cccgcgtgga tgaagttcgg cggcgggcga
    29041 gcgattattt agccattgag atcccataaa cacggctacc ggatgcggcg taaacgctgc
    29101 atccggcatc ggcgcaaaaa acacatcgtc aactaaacaa catcgcgatt ttgccgagag
    29161 cataacctat caccagcacc gttaaactga ttttgataat gctggtaatg ctctgtggtt
    29221 tttcatcctc atcatttatt tcttcatggt tttcaggtgt tggctcatcg acatcttcat
    29281 cctccggctc gagcttcact ttcgtcaaca gcattcgggt gataatggtc tctaatggat
    29341 gtgcctctcc gcgttccagc cgcgcgtcca gacgggccag catctcttgt ttcggaaacg
    29401 cttcaagtac tacctgttcc gctacccgat acaaatccag taacggtgaa tcttgctctg
    29461 tctcgctacc gactctcgat ttccaccacg cccatgcttt gcgtttgaag aataacgact
    29521 caataaacga ttgacctaac ggcgataatt ttccgctatg taaaatctcc gcccataaca
    29581 gccgcgtccg ggcgctctca ccaggaacat atttcaacgg cttaccttgc gcatctacca
    29641 tgctccgcgc ttcgataacc atcactaata acgcgaaata atccgggcaa tgacgcttgc
    29701 tccactgcaa caacaaatta tcggcttgtg tagatgggta actttcccgg tacgcgcaga
    29761 gatcggcaag caggctttcc ccctcgccgc agtacaaccg ttgtgcgcac tggtaataac
    29821 gggcatcttc gctttctggt tcttccgctt gccactgttg agcgacagga atgagttccg
    29881 ccatacctgt ttgcaccgag ctgtaccagc gcaacagttt gcgatgcaac ttcgcatcat
    29941 caggaatgaa ccacggacct tccatcgcca aaaacgcagt gacatagtca ggatggtaac
    30001 ggcagcaacg ttccagcgag aagtaatact caaccgtctg gttctgcaac gcaacgggat
    30061 attccagaag gctaaggaaa tcgaaacaat caccagcctg aatggcctca agaaaagcct
    30121 ccctttcttc ctcatctatt ccttcgcagt cattaaacga atgccagttc aaacgctctg
    30181 ccagcaaaga agcacaattt aatgaaagat atcgcgcttc tatagcgatt gcacacagcg
    30241 gccagcgtaa ttgatcgaca tcctccatgt tccaggtatt taattgctga ataaatttct
    30301 gccaggcgga gggctgaaaa cgatcatttt ctgaatccag taatgccctg aatacacgta
    30361 gtatttcatg ctcagtggca gcatcttttt cttcatcatc tgcttcctcg acaggattta
    30421 ccgccagtcg cagcgcctct tcataggcct ggcgtaatgc tttaaaccct tgtggatcgg
    30481 tttcgggatg acataacggt aagcgagcaa gataagcctg gcggataatg tctatttgcg
    30541 tcgtgctttc aatttcgagg atttgccaac atgttttcat aggtcgctca catccttaag
    30601 cgttaccggg cattcaggct ggacagttaa tggctgccag ggaagcgtgg catagaaacg
    30661 gtcgcttccc gctgccagct cttcgaacat ccgactcccg gcgctatcgt atttgtacag
    30721 ggttagcgcc tcgcgtagcg aagcgttgtc ttcgcactga gaagattgcc agtagagacg
    30781 ccctaacgaa taggcagaaa aatagtgcat ccagctgtga tagtaatggt gtgctcgtat
    30841 atagacgcgc gattgcagcc ataaactctc gtcttccgtc aaccacttgt tgagcacacc
    30901 aacccgtgat aaaaaaccca tccgcacata atcccaggct ttgattcccc cttccccaca
    30961 acacatagcg gtttgggcaa cgaattgggt ataaatcaac cctctctccg agccgccagc
    31021 aattaatgct ttccactctt caggggaaga gcgatgccat gtcagatata agccagctaa
    31081 atcattggca tgaccatcat cggtcatctg ataaatagtc tgaattaaat catcacgatt
    31141 atttattccc caggatgatg agagatcgaa ttcttttatc ccggtataca tttttggcga
    31201 gtcatgcgcg ggcttgcaac cctgattaag tatttccatg ggcgtagaaa gcgc
//
LOCUS       DAHHTG010000051        30971 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.051, whole genome shotgun sequence.
ACCESSION   DAHHTG010000051 DAHHTG010000000
VERSION     DAHHTG010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 30971)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30971)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30971
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.051"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            complement(64..1260)
                     /locus_tag="NBI74_002227"
     CDS             complement(64..1260)
                     /locus_tag="NBI74_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001161853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular biosynthesis protein"
                     /protein_id="HCD1932554.1"
                     /translation="MQGNALTVLLSGKKYLLLQGPMGPFFSDVAEWLESLGRNAVNVV
                     FNGGDRFYCRHRHYLAYYQTPKEFPGWLRDLHRQYDFDTILCFGDCRPLHKEAKRWAK
                     SKGIRFLAFEEGYLRPQFITVEEGGVNAYSSLPRDPDFYRKLPDMPAPHVENLTPSTM
                     KRIGHAMWYYLIGWHYRHEFPRYRHHKSFSPWYEARCWVRAYWRKQLYKVTQRKVLPR
                     LMNELDQRYYLAVLQVYNDSQIRNHSNYNDVRDYINEVMYSFSRKAPKESYLVIKHHP
                     MDRGHRLYRPLIKRLSKEYGLGERVIYVHDLPMPELLRHAKAVVTINSTAGISALIHN
                     KPLKVMGNALYDIKGLTYQGHLHQFWQADFKPDMKLFKKFRGYLLVKTQVNGVYYGGR
                     CFTITL"
     gene            complement(1295..3322)
                     /locus_tag="NBI74_002228"
     CDS             complement(1295..3322)
                     /locus_tag="NBI74_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000579243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide biosynthesis protein"
                     /protein_id="HCD1932555.1"
                     /translation="MIGIFSSGIWRIPNLEKFLAQPCQKLSLLRPVPQEVDAIAVWGH
                     RPSAAKPVAIAKAAGKPVIRLEDGFVRSLDLGVNGAPPLSLVVDDCGIYYDASKPSAL
                     EKLVQDKAGNAALADQAREAMHTIVTEDLSKYNLAPAFVADESEHSDIVLVVDQTFND
                     MSVTYGNAGQNEFAAMLEAAIAENPQAAIWVKVHPDVLEGKKTGYFADLRATQRVRLI
                     AENVSPQSLLRHVSRVYVVTSQYGFEALLAGKPVTCFGQPWYAGWGLTDDRHPQSALL
                     SARRGSATLEELFAAAYLRYCRYIDPQTGAVSDLFTVLQWLQLQRRHLQQRNGYLWAP
                     GLTLWKSAILKPFLRTPTNRLSFSRRCTAASACVVWGVKGEQQWRAEAQRNSLPLWRM
                     EDGFLRSSGLGSDLLPPLSLVLDKRGIYYDATRPSDLEVLLNHSQLTLAQKMRAEKLR
                     QRLVESKLSKYNLGADFSLPAEAKDKKVILVPGQVEDDASIKTGTVSIKSNLELLRTV
                     RERNPHAYIIYKPHPDVLVGNRKGDIPAEMIAELADYQALDADIIQCIQRADEVHTMT
                     SLSGFEALLHGKLVHCYGLPFYAGWGLTVDEHRCPRRERELTLADLLYQALIVYPTYI
                     HPTRLQPITVEEAAEYLIQTPRKPMFITRKKAGRVIRYYRKLIMFCKVRFG"
     gene            complement(3319..4059)
                     /gene="kdsB"
                     /locus_tag="NBI74_002229"
     CDS             complement(3319..4059)
                     /gene="kdsB"
                     /locus_tag="NBI74_002229"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001578967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HCD1932556.1"
                     /translation="MSKAVIVIPARYGSSRLPGKPLLDIVGKPMIQHVYERALQVAGV
                     AEVWVATDDPRVEQAVQAFDGKAIMTRTDHESGTDRLVEVMHKVDADIYINLQGDEPM
                     IRPQDVETLLQGMRDDPALPVATLCHAISAEEATEPSTVKVVVNTRHDALYFSRSPIP
                     YPRNAEKARYLKHVGIYAYRRDVLQNYSQLPESMPEQAESLEQLRLMNAGINIRTFEV
                     AATGPGVDTPACLAKVRALMAQELAENA"
     gene            complement(4069..5745)
                     /locus_tag="NBI74_002230"
     CDS             complement(4069..5745)
                     /locus_tag="NBI74_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016159830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HCD1932557.1"
                     /translation="MKLFKSILLIAACHAAQASAAIDINADPKLTGAAPLTGILNGQQ
                     SDTQNMSGFDNTPPPTPPVVMSRMFGAQLFNGTSADSGATVGFNPDYILNPGDSIQVR
                     LWGAFTFDGALQVDPKGNIFLPNVGPVKVAGVSNSQLNALVTSKVKEVYQSNVNVYAS
                     LLQAQPVKVYVTGFVRNPGLYGGVTSDSLLNYLIKAGGVDPERGSYVDIVVKRGNRVR
                     SNVNLYDFLLNGKLGLSQFADGDTIIVGPRQHTFSVQGDVFNSYDFEFRESSIPVTEA
                     LSWARPKPGATHITIMRKQGLQKRSEYYPISSAPGRMLQNGDTLIVSTDRYAGTIQVR
                     VEGAHSGEHAMVLPYGSTMRAVLEKVRPNSMSQMNAVQLYRPSVAQRQKEMLNLSLQK
                     LEEASLSAQSSTKEEASLRMQEAQLISRFVAKARTVVPKGEVILNESNIDSVLLEDGD
                     VINIPEKTSLVMVHGEVLFPNAVSWQKGMTTEDYIEKCGGLTQKSGNARIIVIRQNGA
                     AVNAEDVDSLKPGDEIMVLPKYESKNIEVTRGISTILYQLAVGAKVILSL"
     gene            complement(5769..6917)
                     /locus_tag="NBI74_002231"
     CDS             complement(5769..6917)
                     /locus_tag="NBI74_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000905920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule polysaccharide export inner-membrane
                     protein KpsE"
                     /protein_id="HCD1932558.1"
                     /translation="MLIKVKSAVSWMRARLSAISLADIQKHLAKIIILAPMAVLLIYL
                     AIFSQPRYMSESKVAIKRSDDLNSGSLNFGLLLGASNPSSAEDALYLKEYINSPDMLA
                     ALDKQLNFREAFSHSGLDFLNHLSKDETAEGFLKYYKDRINVSYDDKTGLLNIQTQGF
                     SPEFALKFNQTVLKESERFINEMSHRIARDQLAFAETEMEKARQRLDASKAELLSYQD
                     NNNVLDPQAQAQAASTLVNTLMGQKIQMEADLRNLLTYLREDAPQVVSARNAIQSLQA
                     QIDEEKSKITAPQGDKLNRMAVDFEEIKSKVEFNTELYKLTLTSIEKTRVEAARKLKV
                     LSVISSPQLPQESSFPNIPYLIACWLLVCCLLFGTLKLLLAVIEDHRD"
     gene            complement(6989..7972)
                     /locus_tag="NBI74_002232"
     CDS             complement(6989..7972)
                     /locus_tag="NBI74_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001327875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HCD1932559.1"
                     /translation="MSERHLPDDQSSTIDPYLITSVRQTLAEQSAALQNLSKQLDSGQ
                     YQRVLNLIMNCKGHVILSGMGKSGHVGRKMSATLASTGTPSFFIHPAEAFHGDLGMIT
                     PYDLLILISASGETDEILKLVPSLKNFGNRIIAITNNGNSTLAKNADAVLELHMANET
                     CPNNLAPTTSTTLTMAIGDALAIAMIHQRKFMPNDFARYHPGGSLGRRLLTRVADVMQ
                     HDVPAVQLDASFKTVIQRITSGCQGMVMVEDAEGGLAGIITDGDLRRFMEKEDSLTSA
                     TAAQMMTREPLTLPEDTMIIEAEEKMQKHRVSTLLVTNKANKVTGLVRIFD"
     gene            complement(8598..9353)
                     /gene="istB"
                     /locus_tag="NBI74_002233"
                     /pseudo
     CDS             complement(8598..9353)
                     /gene="istB"
                     /locus_tag="NBI74_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001522280.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element ISEc10 family helper ATPase
                     IstB"
     gene            complement(9350..10849)
                     /gene="istA"
                     /locus_tag="NBI74_002234"
     CDS             complement(9350..10849)
                     /gene="istA"
                     /locus_tag="NBI74_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000174402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element ISEc10 family transposase"
                     /protein_id="HCD1932560.1"
                     /translation="MTLNTSQVSYYMTQRKKGITQHISAMKAGISVRSGRRIEKGEWA
                     KNSVRHWRTRKDPLEAVWDSMLVPLLKERPALTPTTLLEMLQDKYPGQYPNSLRRTMQ
                     RRVREWKLQYGAEQEVMFRQRHQPGLRGLSDFTELKGVVVTIAGKLLAHKLYHFRLEW
                     SHWSWMRVVLGGESFSALAEGLQEALGQLGGVPVEHKTDSLRAAWKQQGEDGRRELTE
                     RYAALCQHYGMQGVHNNAGRGHENGSVESAHGHLKRRICQALILRGSNDFSTIEEYQA
                     FITQQVMRHNRNNQDLVKEERLHLKPLPLRRSADYDELTVRVSRSSTINVKHVVYSVP
                     SRLVGQLLRVRLWDDRLSCYVGSSEVMSCPRVRPEKGKTRARRIDFRHVIDSLAKKPG
                     AFCHATLRNDILPDDEWRRLWRRLCNHLEPDMAGRLMVHALKLAAGYDDISVVAKGME
                     QMLNTPGNVDLHRLMRFLGIKEKALPVVNVIQHNLSSYEQLLRGKGGSQ"
     gene            complement(11196..11351)
                     /locus_tag="NBI74_002235"
     CDS             complement(11196..11351)
                     /locus_tag="NBI74_002235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1932561.1"
                     /translation="MAALVVTWFNPVKEAFYMHLPAPGKTKKVALVDCMRKLLTILNA
                     MLRKNEE"
     gene            complement(11783..12717)
                     /locus_tag="NBI74_002236"
                     /pseudo
     CDS             complement(11783..12717)
                     /locus_tag="NBI74_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536504.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
     gene            complement(12710..13105)
                     /locus_tag="NBI74_002237"
     CDS             complement(12710..13105)
                     /locus_tag="NBI74_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000208382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HCD1932562.1"
                     /translation="MTTKDRIQSRLNRSKRYVFTRDDFRDIAGYDQVGRALRTLVNEG
                     KLMKVGYGVYTKARRNIITGKIMPAAPGGSAAVITEALERLNIRYRLAGATECYNKGQ
                     STQIPVSLGIKTPSRFKRVLIVGKSKINA"
     gene            complement(13174..14019)
                     /locus_tag="NBI74_002238"
     CDS             complement(13174..14019)
                     /locus_tag="NBI74_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
                     /protein_id="HCD1932563.1"
                     /translation="MSDITASRPEVVNGHTEVICSTSIHHILAVRKSTLLQIDTLIRQ
                     LAEISVLTESIGGKTTRDWAMKQDFRCGCWLMEKPETAMKAITRNLDREIWRDLMQRS
                     RMLSLMDAQARDTWYRSLEYDNFPEISEANILSTFKQLHQNKDEVFERGVINVFRGLS
                     WNYKTNSPCKFGSKIIINNLVRWDRWRFHLITGQQTDRLADLERMLHLFSDKPIPDNR
                     ENITIRLDGHIQSVQGKERYEDEMFSIRYFKKGSAHITFRKPELVDRLNDIIARHYPN
                     VLADR"
     gene            complement(14104..14301)
                     /locus_tag="NBI74_002239"
     CDS             complement(14104..14301)
                     /locus_tag="NBI74_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF957 domain-containing protein"
                     /protein_id="HCD1932564.1"
                     /translation="MKSLTTETALDILIAWLQDNIDCESGIIFDNDEDKTDSAALLPC
                     IKQAREDIRTLRQQQLLQQNR"
     gene            complement(14318..14805)
                     /locus_tag="NBI74_002240"
                     /pseudo
     CDS             complement(14318..14805)
                     /locus_tag="NBI74_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392970.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(14802..15179)
                     /locus_tag="NBI74_002241"
     CDS             complement(14802..15179)
                     /locus_tag="NBI74_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin"
                     /protein_id="HCD1932565.1"
                     /translation="MKTLPDTHVRAASRCPSPVTIWQTLLTQLLDQHYGLTLNDTPFA
                     DERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGFSAGAPSQLINSIDILRARRATG
                     LMTRNNYKMVNNITQGKHPEAKR"
     gene            complement(15256..15636)
                     /locus_tag="NBI74_002242"
     CDS             complement(15256..15636)
                     /locus_tag="NBI74_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001545732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system YeeU family
                     antitoxin"
                     /protein_id="HCD1932566.1"
                     /translation="MSDTLPGTTLPDDNKDRPWWGLPCTVTPCFGARLVQEGNRLHYL
                     ADRAGIRGRFNNADAYHLDQAFPLLMKQLELMLTSGELNPRHQHTVTLYAKGLTCDAD
                     TLGSCGYVYLAVYPATETESNPPE"
     gene            complement(15686..16330)
                     /locus_tag="NBI74_002243"
     CDS             complement(15686..16330)
                     /locus_tag="NBI74_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000086751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin of toxin-antitoxin stability system"
                     /protein_id="HCD1932567.1"
                     /translation="MSRIITTTVYTLHELSSTAQEKARDWYRQHHADSNWYENVYEDF
                     REVCGIFGIDLRQRVFRLSNGRFMEEPCIWFSGFCGQGDGACFEGRWHWQPAAPRKIR
                     EYAPQNRELHRISDALQVVQKRNFWQLQTEIHHRGRDCHPYSMDITVTRNSPTGQAMT
                     TDAEAAVSEALRDLAFWLYRQLENEYDWLTSDAAVDEALLINEYTFTEAGLRAG"
     gene            complement(16349..16570)
                     /locus_tag="NBI74_002244"
     CDS             complement(16349..16570)
                     /locus_tag="NBI74_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 domain-containing protein"
                     /protein_id="HCD1932568.1"
                     /translation="MKIITRGEAMRIHRQHPASRLFPFCTGKYRWHGSAEAYTGREVQ
                     DIPGVLAVFAERRKDSFGPYVRLMSVTLN"
     gene            complement(16639..17115)
                     /locus_tag="NBI74_002245"
     CDS             complement(16639..17115)
                     /locus_tag="NBI74_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RadC family protein"
                     /protein_id="HCD1932569.1"
                     /translation="MQQLSFLPGEMTPGERSLIQRALKTLDRHLHEPGVAFTSTHAAR
                     EWLILNMAGLEREEFRVLYLNNQNQLIAGETLFTGTINRTEVHPREVIKRALYHNAAA
                     VVLAHNHPSGEVTPSKADRLITERLVQALALVDIRVPDHLIVGGNQVFSFAEHGLL"
     gene            complement(17131..17616)
                     /locus_tag="NBI74_002246"
     CDS             complement(17131..17616)
                     /locus_tag="NBI74_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HCD1932570.1"
                     /translation="MKTISHNSTTSSVSVTAASGNDQPQLVATLVPDEQRISFWPQHF
                     GLIPQWVTLEPRVFGWMDRLCEDYCGGIWNLYTLNNGGAFMAPEPDDDDDETWVLFNA
                     MNGNRAEMSPEAAGIAACLMTYSHHACRTECYAMTVHYYRLRDYALQHPECSAIMRII
                     D"
     gene            complement(17671..18489)
                     /locus_tag="NBI74_002247"
     CDS             complement(17671..18489)
                     /locus_tag="NBI74_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF945 domain-containing protein"
                     /protein_id="HCD1932571.1"
                     /translation="MRLASRFGYANQIRRDRPLTHEELMHYVPGIFGEDKHTSRSQNY
                     TYIPTITVLESLQREGFQPFFACQTRVRDPGRRGYTKHMLRLRRAGEINGEHVPEIIL
                     LNSHDGTSSYQMLPGYFRFVCQNGCVCGQSLGEVRVPHRGNVVDRVIEGAYEVVGVFD
                     RIEEKRDAMQSLILPPPARQALAQAALTYRYGDEHQPVTTADILTPRRREDYGKDLWS
                     AYQTIQENMLKGGISGRSAKGKRIHTRAIHSIDTDIKLNRALWVMAETLLESLR"
     gene            complement(18589..18822)
                     /locus_tag="NBI74_002248"
     CDS             complement(18589..18822)
                     /locus_tag="NBI74_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001119724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 family protein"
                     /protein_id="HCD1932572.1"
                     /translation="MPGYTEYVLAEGSFSYGQAVAVITAYRNVFIQDDPGMHFRRVIR
                     NAEGQRRWRCRNSEPDAGKVLNTRLASDGLLRQ"
     gene            complement(18901..19356)
                     /locus_tag="NBI74_002249"
     CDS             complement(18901..19356)
                     /locus_tag="NBI74_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1932573.1"
                     /translation="MIHLFKTCMITAFILGLTWSAPLRAQDQRYISIRNTDTIWLPGN
                     ICAYQFRLDNGGNDEGFGPLTITLQLKDKYGQTLVTRKMETDAFGDSNATRTTDAFLE
                     TECVENVATTEIIKATEESNGHRVSLPLSVFNPQDYHPLLITVSGKNVN"
     gene            complement(19432..21948)
                     /locus_tag="NBI74_002250"
     CDS             complement(19432..21948)
                     /locus_tag="NBI74_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1932574.1"
                     /translation="MLQIVGALILLIAGFAILRLLFRALISTASALAGLILLCLFGPA
                     LLAGYITERITRLFHIRWLAGVFLTIAGMIISFMWGLDGKHIALEAHTFDSVKFILTT
                     ALAGGLLAVPLQIKNIQQNGITPEDISKEINGYYCCFYTAFFLMACSACAPLIALQYD
                     ISPSLMWWGGLLYWLAALVTLLWAASQIQVLKKLTCAISQTLEEQPVLNSKSWLTSLQ
                     NDYSLPDSLTERIWLTLISQRISRGELREFELADGNWLLNNAWYERNMAGFNEQLKEN
                     LSFTPDELKTLFRNRLNLSPEANDDFLDRCLDGGDWYPFSEGRRFVSFHHVDELRICA
                     SCGLTEVHHAPENHKPDPEWYCSSLCRETETLCQEIYERPYNSFISDATANGLILMKL
                     PETWSTNEKMFASGGQGHGFAAERGNHIVDRVRLKNARILGDNNARNGADRLVSGTEI
                     QTKYCSTAARSVGAAFDGQNGQYRYMGNNGPMQLEVPRDQYAGAVETMRNKIREGKVP
                     GVTDPAEASRLIRRGHLTYTQARNITRFGTIESVTYDIAEGSVVSLAAGGISFALTAS
                     VFWLSTGDRDAALQTAAVQAGKTFTRTLAVYVTTQQLHRLSVVQGMLKHIDFSTASPT
                     VRLALQKGTGAGNISALNKVMKGTLVTSLALVAVTTGPDMIKMLRGRISGTQFIRNLA
                     VASSGVAGGAVGSVAGGILFSPLGPFGALTGRVVGGVLGGMIASAVSGKIAGALVEED
                     RVKILAMIQEQVTWLAGSFLLTGHEIENLNENLARVIDQNALEIIFAAGIQQRAATNM
                     LIKPLVVSIIRQRPVMEYDASHLGNMVNRLEEALPPELPA"
     gene            complement(22069..25188)
                     /gene="ag43"
                     /locus_tag="NBI74_002251"
     CDS             complement(22069..25188)
                     /gene="ag43"
                     /locus_tag="NBI74_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter adhesin Ag43"
                     /protein_id="HCD1932575.1"
                     /translation="MKRHLNTSYRLVWNHITGTLVVASELARSRGKRAGVAVALSLAA
                     VMSVPALAADIVVRPGETVNGGTLANHDNQIVFGTTNGMTISTGLEYGPDNEANTGGQ
                     WVQDGGTANKTTVTSGGLQRVNPGGSVSDTVISAGGGQSLQGRAVNTTLNGGEQWMHE
                     GAIATGTVINDKGWQVVKPGTVATDTVVNTGAEGGPDAENGDTGQFVRGDAVRTTINK
                     NGRQIVRTEGTTNTTVVYAGGDQTVHGHALDTTLNGGYQYVHNGGTASDTVVNSDGWQ
                     IVKNGGVAGNTTVNQKGRLQVDAGGTATNVTLKQGGALVTSTAATVTGINRLGAFSVV
                     EGKADNVVLENGGRLDVLTGHTATNTRVDDGGTLDVRNGGTATTVSMGNGGVLLADSG
                     AAVSGTRSDGKAFSIGGGQADALMLEKGSSFTLNAGDMATDTTVNGGLFTARGGTLAG
                     TTTLNNGAILTLSGKTVNNDTLTIREGDALLQGGALTGNGRVEKSGSGTLTVSNTTLT
                     QKAVNLNEGTLTLNNSTVTTDVIAQRGTALKLTGSTVLNGAIDPTNVTLTSGATWNIP
                     DNATVQSVVDDLSHAGQIHFTSARTGKFVPTTLKVKNLNGQNGTISLRVRPDMAQNNA
                     DRLVIDGGRATGKTILNLVNAGNSASGLATSGKGIQVVEAINGATTEEGAFIQGNKLQ
                     AGAFNYSLNRDSDESWYLRSENAYRAEVPLYASMLTQAMDYDRILAGSRSHQTGVSGE
                     NNSVRLSIQGGHLGHDNNGGIARGATPESSGSYGFVRLESDLLRTEVAGMSLTTGVYG
                     AAGHSSVDVKDDDGSRAGTVRDDAGSLGGYMNLTHTSSGLWADIVAQGTRHSMKASSG
                     NNDFRARGRGWLGSLETGLPFSITDNLMLEPRLQYTWQGLSLDDGKDNAGYVKFGHGS
                     AQHVRAGFRLGSHNDMTFGEGTSSRAPLRDSAKHSVRELPVNWWVQPSVIRTFSSRGD
                     MRVGTSTAGSGMTFSPSQNGTSLDLQAGLEARVRENITLGVQAGYAHSVSGSSAEGYN
                     GQATLNVTF"
     gene            complement(25516..26388)
                     /locus_tag="NBI74_002252"
     CDS             complement(25516..26388)
                     /locus_tag="NBI74_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase family protein"
                     /protein_id="HCD1932576.1"
                     /translation="MNPSDAIEAIEKPLSSLPYSLSRHILEHLRKLTRHEPVIGIMGK
                     SGAGKSSLCNALFQGEVTPVSDVHAGTREVRRFRLSGHGHSMVITDLPGVGESRDRDA
                     EYEALYRDILPELDLVLWLIKADDRALSVDEYFWRHILQCGHQQVLFVVTQADKTEPC
                     HEWDMAGIQPSPAQAQNIREKTEAVFRLFRPVHPVVAVSARTGWELDTLVSALMTALP
                     DHAASPLMTRLQDELRTESVRSQAREQFTGAVDRIFDTAESVCVASVVRTALRAVRDT
                     VVSVARAVWNWIFF"
     gene            27720..29210
                     /locus_tag="NBI74_002253"
     CDS             27720..29210
                     /locus_tag="NBI74_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001513421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3987 domain-containing protein"
                     /protein_id="HCD1932577.1"
                     /translation="MPYFQYPDEFPLSSLPPLIRDAVIEAQQITQAPLGLVAASALGA
                     VSLVCQNLIDVCRLNTLRGPVSLFLLTLAESGERKTAVDKLLMEPLYQQEMLLYSRHK
                     NELTTWKNKEELLKAQKKALLSKLNKELRKGADESETLRQLEALQKNRGEKPVRYKFI
                     FNDATTAAIKDQLCGQWRSVGIMSDEAGIIFDGYTLSELPFINKMWDGSVLSVDRKNE
                     PEQMIENARMTLSLMVQPGLFDRYMERKGSVARDSGFLARCLISKPATTQGKRFINGA
                     VTPGGSLTAFHERLMELARGSIEKSSKDERYCLHFSPEAQKIFIDHYNVLEQDLSPSG
                     PLSQFRGHVSKKTENIARIAALLQYFSQGEGKISADIMTSAVVISSWYTDEYKKLFAL
                     PDESELQLQDARELLDWLIEECRGECPPRVRKNYILQCGPGRFRNRKKLNVLLNILAS
                     QLRLSVVQEGKTTYVLLSEINNITLPELNACYSQKLIRWHEQSFGL"
     gene            complement(29331..29903)
                     /locus_tag="NBI74_002254"
     CDS             complement(29331..29903)
                     /locus_tag="NBI74_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1932578.1"
                     /translation="MQKLENRCELLLIQHQKWMTNVTRLIVAHGMGAPHLHGYHRLTL
                     AHFFLPEKGTIISVAPQGLYQVVNPGTPPFIPAIQEGLMTSIQTHEIMLLTHFNLGGV
                     LLSELHRLGENRLANRLNSLLRRFDDRDLYHTLIWLCWYDLMCAHSMQPWTEELKHKS
                     HAELENWAVARKREKRELELMIDEYLLYAC"
     gene            complement(29989..30195)
                     /locus_tag="NBI74_002255"
     CDS             complement(29989..30195)
                     /locus_tag="NBI74_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family transcriptional regulator"
                     /protein_id="HCD1932579.1"
                     /translation="MNTPVSLMDDQMVDMSFITQLTGLTDKWFYKLIKDGAFPAPIKL
                     GRSSRWLKSEVEAWLQARITQSRP"
BASE COUNT         7342 a         7873 c         7750 g         8006 t
ORIGIN      
        1 atgttttgtt aaaaattatt aatcttcaaa cttgacgtgt aaaaccaaaa aaatccttat
       61 ctttcataat gttatggtaa aacatctacc cccataataa accccattaa cctgcgtctt
      121 caccaataaa tacccacgaa acttcttaaa cagtttcata tctggtttaa aatcagcctg
      181 ccagaactgg tgcaaatgcc cttgatacgt caagcctttg atgtcgtaca gggcattgcc
      241 catcacttta agtggtttgt tatgaatcag cgcagagatc cccgccgtac tgttaatcgt
      301 caccaccgct tttgcgtggc gtaataattc cggcatcggg agatcgtgca cataaatgac
      361 tcgctcacct aagccatatt ccttacttag ccgcttaatt aatggtcgat agagtctgtg
      421 accacgatcc atcggatgat gtttgatcac caaataactt tctttcggcg ctttacgcga
      481 aaatgagtac atgacttcat taatatagtc acgtacatcg ttataattgc tgtggttacg
      541 aatctggcta tcgttatata cctgcaaaac ggcaagataa taacgttgat ccaactcgtt
      601 cattaacctc ggtaatacct tacgctgtgt taccttgtaa agttgcttgc gccagtacgc
      661 acgaacccag caacgagcct cataccaggg agaaaatgat ttgtgatggc gatagcgagg
      721 aaactcatga cggtaatgcc agcctatcag gtaataccac atcgcatggc caatacgttt
      781 cattgttgaa ggtgttaagt tctcaacatg cggcgcaggc atatctggta acttacgata
      841 aaaatccgga tcgcgcggta gcgatgaata tgcgttcact ccgccttctt caacagtaat
      901 aaattgcggg cgtaaatatc cttcttcaaa tgccaggaag cggatacctt tcgactttgc
      961 ccagcgtttt gcttctttat gcaatgggcg gcagtcgcca aagcagagga tagtgtcaaa
     1021 gtcatattgc cggtggagat ctcgcaacca tccgggaaac tctttcggtg tctggtagta
     1081 agccagataa tgtcgatggc ggcagtaaaa acgatccccg ccgttgaata caacattcac
     1141 agcgttacgg cctaatgact ctagccactc ggcgacatca ctgaaaaagg gtcccattgg
     1201 cccctgcaat agcagatatt ttttaccgga taataaaacg gttagtgcat taccttgcat
     1261 aaataatttg cgtaatagtc aataatgtaa ttgtttagcc aaatctgacc ttgcagaaca
     1321 taattaattt gcggtaatag cgtattactc gccccgcttt ttttctggta ataaacatcg
     1381 gcttgcgcgg agtctggatc aaatattccg ccgcctcttc aaccgtaata ggttgtagcc
     1441 gtgttgggtg gatataggtt ggataaacaa ttagcgcctg atatagcaga tccgctaacg
     1501 ttaattctcg ctcgcgacgc gggcagcgat gttcatcaac ggttaaaccc caaccggcat
     1561 agaagggcag gccgtaacaa tgtacgagct tgccatgtaa taatgcttca aaccccgaca
     1621 gcgacgtcat ggtatgcact tcatctgcgc gctgaatgca ttgaataata tctgcgtcca
     1681 gtgcctgata atcagcaagt tcagcaatca tttctgccgg aatatcgccc ttgcgattcc
     1741 ccaccagtac atccgggtgc ggtttataaa taatgtaggc gtgcggatta cgctcgcgta
     1801 ctgtgcgtaa taactcaagg ttgctcttaa tcgagacagt gcctgtttta atagaggcat
     1861 cgtcctctac ctgacccggc accaggataa cttttttatc tttggcttcg gctggtagag
     1921 agaaatcggc tcccaggttg tacttgctca gtttgctttc aaccagtcgc tggcgtaatt
     1981 tttcagctcg catcttctgc gccagcgtta gctggctatg attcagcagc acctccagat
     2041 cgctggggcg tgtggcgtca tagtaaatac cacgtttatc cagcaccagc gatagcggcg
     2101 gcagcaggtc agagccaagt ccggatgaac gcagaaaacc atcttccatt cgccataatg
     2161 gcagtgagtt tcgctgcgct tcggctcgcc attgctgctc cccctttaca ccccatacca
     2221 cgcaggcgct cgccgcagta cagcgacgtg aaaaactcag ccggtttgtt ggcgttcgta
     2281 agaagggttt caggatcgcc gacttccaca gcgttaagcc tggcgcccat aaatagccat
     2341 tacgctgttg cagatgtcga cgttgtaatt gcagccattg cagcacggta aacagatcgc
     2401 ttactgctcc cgtttgcgga tcgatatagc gacagtaacg caggtatgca gcggcaaaaa
     2461 gttcttccag cgtggcagaa ccgcgtcggg cagataacaa cgccgactgc ggatgacgat
     2521 cgtccgttaa gccccagcct gcataccagg gctgaccgaa gcatgtcacc ggttttcctg
     2581 ccagcaaggc ttcaaagccg tactgggagg tcacgacgta aaccctggaa acgtgtcgca
     2641 acagcgattg cggactgaca ttctccgcaa tcaaacgtac tcgttgcgtg gcgcgcagat
     2701 cggcgaaata acctgttttc tttccttcca gtacatctgg gtgcaccttc acccaaattg
     2761 ccgcttgagg attttccgca atcgcggcct ccagcatggc ggcaaactca ttctgacccg
     2821 cattaccata cgtcactgac atatcattaa atgtctgatc gacaaccaga acgatgtctg
     2881 aatgttctga ctcatcagcc acaaacgcag gcgccagatt atatttcgat aaatcttcgg
     2941 tcacgatggt gtgcattgct tctctggcct gatctgccaa tgccgcattt ccggctttat
     3001 cctgtaccag tttctccagc gccgaaggct tgctggcatc gtagtaaata ccgcaatcat
     3061 ccaccaccag agaaagcggc ggcgcaccat tgacaccaag atccagcgag cgcacaaatc
     3121 catcttccag acggatgacc ggttttcccg ctgctttggc gatggcgact ggtttcgccg
     3181 cgctggggcg atgtccccac acggcgatag catcaacttc ttgcggaaca gggcgcagca
     3241 gagaaagttt ctggcacggt tgcgccagaa atttctccag atttggaata cgccagatgc
     3301 cggacgagaa aatgccaatc atgcgttttc agccagttcc tgtgccatca gagcgcgtac
     3361 ttttgcaaga catgcagggg tgtcaacgcc cggaccggtt gcagcaacct caaacgtgcg
     3421 aatgttgatt cccgcgttca ttaaccgtag ctgctccagt gattccgcct gctccggcat
     3481 ggactccggt agctggctgt agttttgcag cacatcgcga cgataagcgt aaataccgac
     3541 gtgtttcaga tagcgtgctt tttcagcatt acgcggatac ggaatcggcg aacggctgaa
     3601 ataaagtgca tcatggcggg tattcaccac cacttttact gtgctgggct ccgtcgcttc
     3661 ttcggcagaa atcgcgtggc atagcgttgc caccggtaac gcgggatcgt cacgcattcc
     3721 ttgcagcagc gtttctacat cctgcgggcg aatcatgggt tcgtcgccct gcaggttaat
     3781 gtagatatct gcatcgactt tatgcatcac ttcgaccagc cgatcggtgc cggattcatg
     3841 atcggtacgc gtcataatgg ctttcccgtc aaacgcctgt acggcctgtt caacacgcgg
     3901 gtcgtcagtt gccacccaaa cttctgcgac gcccgccacc tgcaacgcac gctcgtaaac
     3961 atgctggatc atcggtttgc caacaatatc aagtagcggc ttacccggca ggcgcgatga
     4021 gccataacga gccggaatga caataactgc tttgctcatt tcaactcctt acaaagacag
     4081 aatcactttt gcacccaccg ccagctgata gaggatggtg gaaataccac gggtaacttc
     4141 aatgtttttc gattcatatt tcggcagaac cataatctca tcgcccggtt tgagtgaatc
     4201 cacatcttct gcgttgacgg ctgcaccgtt ctgacgaatg acgataattc tggcgttacc
     4261 cgatttctgc gtcaggccac cacatttctc gatgtaatcc tcggtggtca tacccttctg
     4321 ccagctcacc gcgttcggga acagcacttc gccatgaacc ataaccagcg atgttttctc
     4381 cggaatattg atgacgtcgc catcttcaag cagaacagaa tcaatattgg attcgttgag
     4441 gatcacttca cctttcggaa caacggtgcg tgctttcgcc acaaagcggc tgatcagttg
     4501 tgcttcctgc attcgcaggc tggcttcttc tttggtggag gactgggcag aaagtgatgc
     4561 ttcctccagc ttttgcagcg agagattcag catctctttc tgacgctgag ccactgatgg
     4621 gcgataaagc tgaactgcgt tcatctgcga catgctgttc gggcggactt tttccagaac
     4681 cgcacgcata gtggaaccat aaggcagtac catggcatgt tcaccggagt gtgcgccttc
     4741 aacccgcacc tgaatggtgc cggcatagcg gtcagtgctc acgattaagg tatcgccatt
     4801 ttgcaacata cggcctggcg cagaactgat cggatagtat tcgctgcgtt tttgcagccc
     4861 ctgtttacgc ataatcgtaa tgtgagtcgc gccaggctta gggcgcgccc agctcaacgc
     4921 ttccgttacg ggaatgctgc tttcgcggaa ctcaaagtcg tagctgttaa agacatcgcc
     4981 ctgaacgctg aaagtatgct gacgcggccc gacgatgatg gtgtcaccat cggcgaactg
     5041 cgaaagcccc agtttgccgt tcagcaggaa gtcgtacagg ttgacgttgg agcgcacgcg
     5101 gttaccgcgc ttgaccacaa tatcaacgta acttccgcgc tctggatcaa cgccgccagc
     5161 cttgatcaga taattgagta acgaatcaga cgtcacaccg ccatacagac caggattacg
     5221 cacaaatccg gtcacgtaca cttttactgg ctgcgcctgt aataaggagg cgtagacgtt
     5281 gacgttggac tggtatactt ccttcacttt ggatgtgacc agggcattta gctgactatt
     5341 actgacgcca gcaactttca ccggaccaac gttcggcagg aaaatattac ctttgggatc
     5401 aacctgtaac gcaccatcaa aggtgaacgc accccacaag cgaacctgaa tgctatcacc
     5461 cggattcaga atatagtcag ggttgaatcc taccgtcgca ccgctatccg cgctggtgcc
     5521 gttgaaaagt tgagcaccaa acatacggct cattaccacc ggcggtgtgg gcggcggggt
     5581 attgtcgaag ccgctcatgt tttgcgtatc cgactgttgc ccgttcagaa taccggtaag
     5641 cggcgcggct cctgtaagtt ttgggtcagc gttaatatca atggccgcgc tggcctgcgc
     5701 cgcgtgacag gcggcaatca gtaaaattga tttaaataat ttcatcacaa actcattcag
     5761 cgacagcgtt agtctcggtg atcttcaata acagccagca acagtttcag ggtgccgaac
     5821 agcaggcagc acaccagtaa ccagcaggcg atcaaataag ggatatttgg aaaagacgat
     5881 tcctgcggca actgtggcga actgatcact gacagcacct tgagcttacg agccgcttct
     5941 acacgggtct tttcaatgga ggtcagggtc agtttgtaca gctcggtgtt gaactctact
     6001 tttgatttga tttcttcaaa atccaccgcc atacggttta gcttgtcacc ctgcggcgct
     6061 gtgattttgc ttttttcttc gtcaatttgt gcctgcaatg actgaatcgc attacgcgca
     6121 ctcacaactt gcggggcgtc ctcacgcaga tacgtcagca agttccgcag gtccgcttcc
     6181 atctggatct tctggcccat cagcgtattc actaacgtgc tcgccgcctg tgcctgtgcc
     6241 tgtggatcca gaacgttgtt gttgtcctga taagagagca attccgcttt gctggcgtcc
     6301 agacgctggc gtgccttttc catctccgtt tctgcaaagg caagctggtc acgcgcgatg
     6361 cgatgtgaca tctcattgat aaagcgctct gactctttca gcacggtctg gttaaactta
     6421 agcgcaaact ccgggctaaa gccctgcgtc tgaatattca gtaatccggt tttatcgtca
     6481 tacgagacgt tgatacggtc cttgtagtac ttcaggaagc cttctgcggt ttcatccttg
     6541 ctaagatgat taagaaaatc gagcccgctg tggctaaacg cttcacgaaa atttagttgc
     6601 ttatccagcg ccgccagcat atccggcgag ttgatgtact ctttcagata caacgcatct
     6661 tctgcggaac tggggttaga ggcacccaga agcagaccaa aattcaggct gccgctgttt
     6721 aaatcatccg agcgtttaat ggcgactttc gactcgctca tatagcgagg ctggctgaag
     6781 atagccagat agatcagcag caccgccatc ggtgccagaa tgatgatttt cgccaggtgt
     6841 ttttggatat ctgccagtga gatggcagac agacgagcac gcatccagga tacggcagac
     6901 ttcactttta tcaacatgta acgattatca aagtttcagt ggggcgcaaa ctcaatgaga
     6961 gactaacctc tcgaaccccg ttgcttaatt agtcgaaaat gcgcacaagg ccagtgactt
     7021 tatttgcctt gttggtcacc aataacgttg agacgcggtg cttttgcatt ttttcttctg
     7081 cttcaatgat catggtgtct tccggtagcg tcagcggttc acgtgtcatc atctgcgcag
     7141 ccgtagcgga tgtcagagaa tcctcttttt ccataaaacg acgcaggtca ccgtcggtga
     7201 taatgcccgc tagcccacct tctgcgtctt ctaccatcac cattccctgg catccgctgg
     7261 tgatacgttg aatcacggtt ttaaatgacg catccagctg taccgcagga acatcatgct
     7321 gcatgacatc agcaacgcgg gtcagcaggc gacgacctaa tgaaccgccc gggtgatagc
     7381 gcgcaaaatc attcggcata aatttgcgtt ggtggatcat ggcaatcgcc agcgcatcgc
     7441 cgatcgccat cgtcagcgtg gtagacgttg ttggtgcaag attattcgga caggtttcat
     7501 tcgccatgtg gagttccaac acggcatcag catttttcgc cagcgtggaa tttccattat
     7561 tggtaatggc gataattcgg ttgccgaaat ttttcagtga aggaactagc ttgaggattt
     7621 catccgtttc accgctggca gaaataagga tcagaagatc gtaaggcgta atcatgccca
     7681 gatcgccatg gaaagcttct gcaggatgaa taaagaaact aggcgtaccc gtagaggcca
     7741 gcgtcgctga cattttacga ccgacatgcc ctgatttacc cattcccgaa agaataacgt
     7801 gccctttaca gttcattatc aaattaagga cacgctggta ctgcccggaa tccagttgtt
     7861 tagacaagtt ttgtaatgct gcgctttgtt ctgccagagt ctggcgaaca gaggtaatta
     7921 gatatggatc gatagtactg ctctggtcat caggtaaatg tctttcagac atatcagctc
     7981 ctttgcacgg aaataatgcc acagtcacaa caaacccgaa cccactataa tttatttatg
     8041 ctaaatcaat gggttgtaac aaaattttta atgaatataa aaccatggtc agtccatgct
     8101 tatatgcagg acttatcacc ttcttattaa gtcgcaatgt ctcatggagg tgcgaagacg
     8161 ctgcaatatt acaggcgaga aagggaacca aattttgccc acctatttaa cacactccta
     8221 cattatgaac aaggaattta ggacttttat catttattaa aatatttcct acttgactat
     8281 taatacaaaa acaatttatc aattgattat ttccttgtaa aaaaagaacg tatgactatg
     8341 ttattaataa ataaaacacg caaataatgt tgatgttgtt ttgtgattaa ttatttaagt
     8401 tcggtataat aaacaggttg acaatttcta tgtaaagaag aggaaaataa taaaatttta
     8461 gcggtgacta ttgaatgata tgatagttgt ggtgttgatt atttctggta atgaatgcgg
     8521 aattaaatct ggctattaga cgtcaactag ttttgccgac tggcgccaac tatactggcc
     8581 gccccttcag tgcgacatca agtcacgctt gttaccgcct tcgctgtctt tttcctgtag
     8641 ctttcccctt tcagttcgaa catatatccg tgatggatca gtcggtctgc tgccgccacc
     8701 gccatagtct catccacgaa gatgctgccc cacatgctga acggatggtt actggttatc
     8761 accaggctcc cacgttcata gcgatgcgct attaactcga acagcacacc cgtttcggcg
     8821 ctgtcgcgtt tgacatagcc aagatcatcc accactatca cccggtagcg atccagtttc
     8881 agtagcagct cattcagttt caactgagct ctggctttac gtagttcctg caacagctct
     8941 cctgcgctgt agaaccgggc ccggtagccc tggcctacta cgccatccac gatcgctgcc
     9001 gccagatggc ttttccccaa cccgctggcc ccgaacagca gaacgttttc tccagcatca
     9061 acccagtctg tcgtttcaca gagttgccgg aactgagctc cattcagttc cggcacctga
     9121 ctgaagtcgt attcgcttag cgttttggca actggcaacc gggcctcctt tttataacga
     9181 cgcagttttt cactctcgcg ccacaggagt tcttcattgc atagcgtcag aagatagcgc
     9241 gatggtgtcc agccttctgc cagtgctcgt ttttccagag cgtgccattc agatccaact
     9301 catgtcaggc gtagtttacg caggctgcgt tcaaggtgat ggatattgct cactgcgaac
     9361 ctcccttgcc acgcagtagt tgctcataac tgctcaggtt atgctgtatc acattgacta
     9421 ccggcaacgc cttttccttt atacccagga agcgcatcag ccggtgcaga tccacgtttc
     9481 ccggggtatt cagcatctgc tccatacctt ttgccacgac tgagatatcg tcgtatcctg
     9541 cagccagttt cagagcatgt accatcagcc tgcctgccat gtcgggttcc agatgattac
     9601 ataagcgacg ccacagcctc cgccattcat cgtctggcag gatgtcattt ctcagcgttg
     9661 catggcagaa cgcaccgggc ttttttgcca gactgtcgat cacatgtcgg aagtcgatac
     9721 gacgggcccg cgtcttccct ttttctggtc tgacacgtgg gcagctcatg acctcgctgc
     9781 tgccaacgta acagctcaga cgatcgtccc ataaccggac ccgtaacagt tgacctacaa
     9841 gccgggaagg tacgctgtag acgacgtgct tcacattgat ggtactgctg cggctaaccc
     9901 tcacagtcag ctcatcatag tcagcactgc gacgaagcgg cagcggtttc agatgaagac
     9961 gttcttcctt gaccagatcc tgattgttac ggttgtgccg cataacctgc tgagtgatga
    10021 aggcctgata ttcttctata gtgctgaagt cgttactgcc ccgcagtatc agcgcctgac
    10081 agatacgcct tttcagatgt ccgtgggcac tttcaaccga gccattttcg tggccccgac
    10141 cggcattatt gtgtacgccc tgcattccgt agtgctgaca gagagcagca taacgctcag
    10201 tcagctcgcg gcgtccatct tcgccctgtt gtttccatgc tgccctcagg ctgtccgttt
    10261 tatgttctac cggcactccg cccagttgtc cgagggcttc ctgcagacct tcagccagag
    10321 cagagaagct ctcaccaccc agcacaaccc gcatccagct ccagtggctc cattccagac
    10381 ggaagtgata caacttatgc gccaacaact taccggcgat ggtgacaact acacctttca
    10441 gttcagtaaa gtccgacaga cctcgcagac cgggctgatg tcgctggcgg aacatgacct
    10501 cctgctctgc accatactgt agcttccatt cgcggacccg ccgttgcatt gttcttcgaa
    10561 ggctgttggg gtactggccg ggatatttat cctgtagcat ctccagcaga gttgttggtg
    10621 tcagagccgg cctctctttc aacagaggaa caagcatgct gtcccacaca gcttccagag
    10681 gatctttgcg tgtgcgccag tgccgaacac tgttttttgc ccactctcct ttttcgatcc
    10741 gacgaccaga acggactgag ataccagcct tcatggccga gatatgctga gttatacctt
    10801 tcttacgctg agtcatatag taactgacct gagaagtatt gagcgtcacg ccatttcctg
    10861 ttggatcatg taaatgacac tcaggttaca aaaccggcca gaataatcgg cgccaggcgg
    10921 actgagttat tgtcgtctca tactggccgg attgtatttt tgctatatct atttttcagg
    10981 agattattat taaatagaaa aataacaatg gtgattatct ccggttgagc tggagtcacg
    11041 tatgccagcg agtgctttct tccgggcctt ccttcacttc agagaaagag cctatggaaa
    11101 agaggcaatc atcaggctct ttttgagtca tatgcgccga taatcaactg tcttgaacgt
    11161 aaaaattatg gagcaacatg atgctacgat tcatttcact cttcgttctt tctgagcatt
    11221 gcgttcagga tggtcagcaa tttacgcata cagtcaacca gcgccacttt tttggttttt
    11281 ccgggtgctg gcagatgcat gtaaaacgcc tctttcaccg gattaaacca ggttaccaca
    11341 agcgctgcca tgtgcagggc tgctcttgcc tccgtacgcg cactggcgtc gttcggccag
    11401 cagatatgtg tgggataaca gggatgttat gtgaactgaa ctgtgttttg ctagcggggg
    11461 atgtttattg atgattcggg agtgtttaag acctgatggc gttatgcacc caagtttatt
    11521 ttatataaag atccaaaagc aaaaacccgc cttgtgggcg ggttcttaag aattagtggt
    11581 gcccggaatc ggaatgaaac tgattgtata acaaattgtt ttatatagat tattttgtat
    11641 ttgttgtttt tgaggccacc aatgaggccc ccatgtgcaa ttcgaattct tttgacgcta
    11701 taatccctct tttttgtgct ttgctgtaat catttggtct ggttcgatat gctgaattgt
    11761 ggaatcgtac ttaacgcgtt tgttagctgg tcagaacact tagcgctgtt tgtctgaaac
    11821 tggaataggc ttcttcccag ttaatctgtg tattaccaaa taccatcggt ttaacaaatc
    11881 gttgaaagcg caatttgtgt acaggataat cccggagtgc atccagccct ttcttaagtt
    11941 catcaactgg tgagctacaa aattgtgggt actggttacc atagcgtgaa tatatatctg
    12001 aacaaaattt ttcaatacag gaacattcac acctttttcc ctgacaatgc agtagttgtc
    12061 gtaaatgtga cgtaccagaa actcatcatc ctgttgttcg ggattacgaa gatgtgctgc
    12121 tgttcttcgc atcatggaga tgagcttttc agcctgagtg gataaaatgg tggcacaggg
    12181 gaatgctgtt acaggtgtac ctttacccgt taactccgtg acaagggaat aaatatctct
    12241 gctttccgga tgttccagca gagtgctttc catcaactcc agcttaataa atggacgaag
    12301 acaggggact tgtgcaaatg tctggggata acgtaccgag atttcgttat aacgatactc
    12361 atcacgtgtt atacgggcgt tttcattatc caagctgaaa atgtcagaat tatgaataac
    12421 atccgtgatt atttgcacga tttccttgcg aatttttctt ctcttgtctc tgccattttg
    12481 cataaagttc tctgttggca cgagctttat atcaatatct tctgacattc tgtttagcga
    12541 gatacctgct tttgataatg cggtacctcc tgcaaacacg agagtatgtg tttctggttt
    12601 tatttctttt aataatctta aaagatcaac cacaaaataa tctttttcga cgatagcgac
    12661 agattcaata tccagtgcat cggcaacgtc aggaaataat gatgttaatt caggcattga
    12721 ttttactttt ccctactatt aacactcttt taaatcgaga aggtgttttt atccccagag
    12781 atacaggaat ttgtgtggac tgacctttat tgtagcattc cgttgctcct gccagacggt
    12841 atctgatatt caggcgctcc agggcctctg taattactgc agcagagccc ccaggcgctg
    12901 cgggcatgat tttcccagtg atgatgttac gacgggcttt ggtgtaaaca ccatagccca
    12961 ctttcatcaa cttaccttcg tttaccagtg tacgaagagc tctgccaacc tgatcatagc
    13021 cagcgatatc cctgaaatca tcccgggtga acacataacg ttttgaacgg ttcagtcggg
    13081 actgaatacg gtcttttgta gtcatgtttc ctccgaaaaa ttttcacaat gatagcaaaa
    13141 atatgacatg tttaatagcg gggcaaggcc acattacctg tctgctaata catttggata
    13201 gtgcctggca attatgtcat ttagcctgtc aaccagttct ggtttcctga acgtgatgtg
    13261 tgcggagccc ttcttaaagt atctgatgct gaacatttcg tcttcatagc gctctttacc
    13321 ctgaacggac tggatgtgac catccagacg aatagtgatg ttttcccggt tgtcgggaat
    13381 cggtttgtcg ctgaacagat gtagcattct ttccagatcg gcgagtcggt cagtctgttg
    13441 tccggtgata agatgaaatc tccaccggtc ccatctcacc agattgttga taatgatttt
    13501 acttccaaat ttacagggag agttggtttt ataattccag ctcagtcccc tgaagacgtt
    13561 gatcactccc cgctcaaaca cctcatcctt gttctggtgc agttgtttaa atgtgctcag
    13621 aatgttcgct tcgctgattt ccggaaaatt atcgtactcc agtgaccggt accatgtatc
    13681 acgggcctgc gcatccatta aggaaagcat cctggaacgt tgcatcaggt cacgccagat
    13741 ttcacggtca aggttgcggg tgatggcttt cattgccgtt tcaggtttct ccatgagcca
    13801 gcagccgcag cgaaaatcct gtttcatggc ccagtcccgg gtggttttac ctccaatgct
    13861 ttccgtcaga actgagattt cagccagttg ccggataagt gtgtcgattt gcagcagtgt
    13921 actttttcgt acagccagaa tgtggtggat actggttgag cagataactt ccgtatgccc
    13981 gttgaccact tccggacggg aggctgtgat atcagacata atcgatattc ctttttgcag
    14041 ataaatgaaa acgccagccc tgttcaggct ggcgtcagtg gaataaagtc tggtgagtaa
    14101 gactcacctg ttctgttgca gaagctgctg ttggcgcagg gtacggatat cttccctggc
    14161 ctgtttaatg cagggcagta gtgctgccga atccgtttta tcctcatcgt tgtcgaagat
    14221 aatccctgat tcgcagtcaa tattgtcctg cagccacgca atcagaatat ccagcgctgt
    14281 ttccgtggtt aatgatttca tgttgtgaat ttccgggtta ccagtcgaaa gtgggtaaat
    14341 ccggcaggca ttcagcgctg gcatccagat gaatgagact gacgccataa cgtcgggtaa
    14401 gtgtgacaac cagacggcgg aacgtttttg acagccccag gcgtttaagc cgcagaaccg
    14461 ggtatgacca cgcatcggta cgtaacagat atccgctacc tgtaaaatga atccattctg
    14521 attcgccggt gtcattcgtc tgatgcgaca gcgagtacag ccaggcgttg tctgtttccg
    14581 tgatatgcgc ggtactacag cgtataccgc tcagggcagc agaggggggg tgctgtctgt
    14641 tgaggtccgg tcagattcat caacaacacg aagggagtag ccattatcgc agaccacgtt
    14701 aatcagttcg gcaagctcaa caccatcgac gtcaacgaca attcgcccca tgttcagtgc
    14761 ctgtacgtta acgctgtcag cttccagcgt cagggccagt ttcatcgttt tgcctccgga
    14821 tgcttgccct gggtaatgtt atttaccatt ttgtaattgt tccgggtcat caggccggtc
    14881 gccctgcgag cccggagaat atcaatgctg ttgattaact gcgacggggc tcctgcgctg
    14941 aatcccggct ggtcggtacg taccagcgcg tatttttcaa cgagaaagtt caccgcgtca
    15001 cacagtgaaa tccctgcctc aatatgctgc tcaatcacac gttcatcagc gaacggtgtg
    15061 tcattcagcg tgaggccata gtgctggtcc agcagttggg tgagcagtgt ctgccagatg
    15121 gtgacgggag acgggcagcg cgatgccgcc cgtacgtgtg tgtcgggtaa tgttttcata
    15181 ctgaagattt tcctgatatg cagatataag aaatggaaaa gtggcgtgga gaaagcacca
    15241 ggccggagca gaagactatt ccggagggtt actttccgtt tcggtcgcag gataaacagc
    15301 cagataaacg taaccacagg acccgagggt gtcggcatcg caggtcagcc ctttcgcata
    15361 cagcgtgacg gtatgctgat ggcggggatt cagttcaccg ctggtgagca tgagttccag
    15421 ttgtttcatc agcagtggaa aagcctggtc cagatggtat gcatccgcat tgttgaaccg
    15481 gcccctgata ccggcgcggt ctgcaaggta atgcaaccgg ttaccctcct gcaccagacg
    15541 ggcgccgaaa cagggcgtca cggtgcaggg cagtccccac caggggcggt ctttgttgtc
    15601 gtcgggaagc gttgttccgg ggagtgtgtc cgacatgata aaatcctcac agaaaatcgg
    15661 tgaatgatag tttaacgatg acgggtcagc cggcgcgcag accggcttcg gtgaaggtat
    15721 attcgttgat gagaagggcc tcatccacag cggcatctga ggtcagccag tcgtactcgt
    15781 tttccagttg acggtacagc cagaaggcca ggtcgcgcag ggcttcgctg actgcggctt
    15841 ccgcatcggt tgtcattgcc tgaccagtcg gactgttccg ggtaaccgtg atatccatgc
    15901 tgtacggatg gcagtcgcgt ccccggtggt ggatttcagt ctgcagttgc cagaagttac
    15961 gtttctgcac cacctgcagg gcatccgaga tacgatgaag ctcacggttc tgcggggcat
    16021 attccctgat tttccggggt gccgctggct gccagtgcca gcgtccttca aaacaggctc
    16081 cgtccccctg gccgcagaat ccgctgaacc agatgcacgg ctcttccata aacctgccgt
    16141 tgctgagccg gaaaacgcgc tggcggaggt caattccgaa aatgccacag acctccctga
    16201 agtcttcata cacgttttca taccagttgc tgtcagcatg atgctgacgg taccagtcgc
    16261 gggctttttc ctgtgcggtg gaggatagct catgcagtgt atacacggtg gtggtgataa
    16321 ttctgctcat cagaatgccc ttactgactc agttcagagt gacgctcatc agccggacat
    16381 acggaccaaa actgtcctta cggcgttctg caaacacggc cagcacaccg ggaatatcct
    16441 gcacttcacg accggtatac gcttcagcac tgccgtgcca gcggtatttg ccggtacaga
    16501 acggaaaaag acgggatgcc ggatgctgac ggtgaatacg catggcttcg ccacgggtga
    16561 tgattttcat agtgggatac ctctgaagac agaagataaa agtgaaaaca ggtgtgatgt
    16621 ggttgtaacg gtgacgggtt aaagcagacc gtgttccgca aaggagaaaa cctggttgcc
    16681 accaactatc agatggtccg gtacccggat atccaccagg gccagtgcct gtaccagacg
    16741 ttccgtgata aggcggtctg ccttgctggg tgtgacttca ccggacgggt gattgtgtgc
    16801 cagcaccaca gcagcggcat tgtgatacag ggcgcgttta atcacttcac ggggatggac
    16861 ttccgtgcgg ttgatggtgc cggtgaagag ggtttcaccg gcaatcagct gattctggtt
    16921 attcagatac agcacccgga attcttcacg ctccagtccc gccatgttca gaatcagcca
    16981 ttcccgtgcc gcatgcgtgg aggtgaaggc cacgccgggt tcatgaagat ggcggtccag
    17041 ggttttcagg gcccgttgaa tgaggctgcg ctcgccgggc gtcatctctc cgggcagaaa
    17101 ggaaagctgt tgcattgtac ttctctccat tcagtcgatg atgcgcataa tggcgctgca
    17161 ttccggatgc tgcagggcgt aatcccgcag ccggtaataa tggaccgtca tggcataaca
    17221 ctccgtacga caggcatgat gactgtacgt catcagacag gcagcaatgc cggcagcttc
    17281 cggactcatt tcagcgcggt taccgttcat ggcattgaac agtacccatg tttcgtcatc
    17341 atcgtcatcc ggttcgggtg ccataaatgc cccgccgttg ttcagggtgt acagattcca
    17401 gataccaccg cagtagtctt cacacagacg gtccatccag ccgaagacac ggggctccag
    17461 ggtcacccac tgtggaatga ggccaaagtg ctgcggccag aagctgatgc gctgttcatc
    17521 agggacgagg gtggcaacca actgaggctg gtcattccct gatgcagcgg ttacggaaac
    17581 agaagatgtg gtggaattat gtgaaatggt tttcatgaaa tgactcctta aaggacaaat
    17641 aaatgcgtgg ttttcagggt aatggctgaa tcagcgcagg ctctccagca gggtttccgc
    17701 catcacccac aatgcgcggt tgagcttaat gtcagtgtca atgctgtgaa tggcacgggt
    17761 atggatacgt tttcctttag cgctgcgacc tgaaattccg cctttcagca tattctcctg
    17821 aatggtctga taagcactcc acaggtcctt accgtaatcc tcccggcgtc gtggcgtcag
    17881 aatgtcggcg gtggtgacgg gctgatgttc gtcaccataa cggtaagtca gtgccgcctg
    17941 tgccagcgcc tggcgtgccg gtggcggcag aatcagcgac tgcatggcat cacgcttttc
    18001 ctcaatccgg tcaaacacgc ccaccacctc gtaagcccct tcaatgactc tgtccaccac
    18061 atttccccgg tgcggaacac gcacttcccc cagagactga ccacagacgc atccgttctg
    18121 gcagacgaac ctgaagtaac ccggcagcat ctggtagctg gaggtaccgt catgagagtt
    18181 gagcagaata atttcaggga catgttctcc gtttatctct ccggcccgcc gcagacgcag
    18241 catgtgtttt gtgtatcccc ggcggcccgg gtcgcgcaca cgggtctggc aggcgaagaa
    18301 tggctgaaag ccttcccgct gcaggctttc cagtacggtg atggtgggga tgtacgtata
    18361 gttctggctt cgggacgtgt gtttatcttc cccgaaaatc cccggcacat agtgcatcag
    18421 ttcttcgtgt gtcagcggac ggtcacggcg tatctggttc gcataaccaa aacgactggc
    18481 tagtcgcata atttgctcct tatcggtggt taagatttac tggtgtaata aatgaaaaag
    18541 ccacgtctcc cggagaagac gcggcctgat gggtgaaatg aatgacgttt attgtctgag
    18601 aagaccgtca ctggcgagcc gcgtattaag cacctttccg gcatcgggtt cagaattccg
    18661 gcagcgccag cgtcgctgac cttccgcgtt gcggataacc cgacggaaat gcattcccgg
    18721 gtcatcctgg ataaacacat tgcggtaagc ggttatgaca gcaaccgcct gcccgtatga
    18781 gaaagatcct tcagccagga catactctgt gtaacccggc atatctgttt tctcctgata
    18841 aataaaaagc ctctgccata cgttgtggca gaggctgaag catgaaactg acttcaggga
    18901 tcagttaaca ttttttccgg aaacggtaat cagcagtgga tggtagtcct ggggattgaa
    18961 aaccgataac ggcagactga cacgatggcc gttactttct tcagttgctt taatgatttc
    19021 ggttgtggcg acattttcca cgcactccgt ttccagaaat gcgtctgtgg ttcgcgtggc
    19081 attactgtca ccaaaagcgt ccgtttccat ttttctggtc accagcgtct gaccatattt
    19141 gtctttgagt tgcagagtga tggtgagggg gccaaatcct tcatcgtttc cgccattatc
    19201 cagccggaac tggtaagcac aaatatttcc cgggagccat atcgtatctg tattgcgtat
    19261 actgatgtaa cgttgatcct gtgcccggag tggggcagac cacgttaacc ccagaatgaa
    19321 ggcggtaatc atgcaggttt tgaacaggtg aatcatggta tttacctctc tgagtcatga
    19381 cgatgacact gacaaatcag gtgataaagc gtaaaaggcg cagaatagcc gttatgccgg
    19441 taactccggg ggtaatgctt cttccagtcg gttaaccata ttgccgagat gggatgcatc
    19501 atattccatg acggggcgtt gcctgatgat actgaccacc agtggtttga ttaacatatt
    19561 ggtcgcggcc cgttgttgta taccggcggc gaaaatgatc tccagagcat tctgatcgat
    19621 aacacgggcc agattctcgt tcagattttc aatctcatgt ccggtcagca ggaaactgcc
    19681 ggcaagccat gtcacctgct cctgaatcat tgccagaatt ttgacgcgat cttcttcaac
    19741 cagcgctccg gcaatttttc ctgatacagc ggaggcaatc attcccccca gaacaccgcc
    19801 aaccacacgc cctgtcagtg caccaaatgg tcccagtgga ctgaacagta tcccgcccgc
    19861 cacagacccg acagctccac ctgccacacc ggaagatgcc acggcaagat tcctgatgaa
    19921 ctgcgtaccg gagatccgtc cccgcaacat tttgatcatg tcagggccgg ttgtgacagc
    19981 taccagtgcc agagatgtca ccagcgtacc cttcatcact ttgttcaggg cactgatatt
    20041 tcctgcaccg gtccccttct gaagagccag tcggacagtc gggctggcag tcgaaaaatc
    20101 aatgtgcttc agcataccct gaacaacact gagccggtgg agttgctggg ttgtgacgta
    20161 gacagccagt gtgcgggtga aggtttttcc tgcctggaca gcagctgtct gcagggcagc
    20221 atcgcgatcg ccggtgctga gccagaagac cgatgccgtc agggcaaaac tgatccctcc
    20281 ggccgccaga ctgacaaccg aaccctcggc aatatcataa gtgactgatt cgatggtccc
    20341 gaaccgggtg atattacggg cctgagtata agtcagatgt ccccgacgaa tcagccgtga
    20401 cgcttctgcg ggatcggtta caccgggtac tttaccttcg cggatcttat tcctcatggt
    20461 ttcaacagcg ccggcatact gatcacgggg gacttccagt tgcatggggc cattatttcc
    20521 catgtaacga tactgtccgt tttgtccgtc gaatgccgca ccgacgctac gggctgcagt
    20581 tgaacaatat ttcgtctgga tttctgttcc gctgaccagt ctgtctgctc catttctggc
    20641 attattatca ccgaggatcc gtgcgttttt cagacggact ctgtcgacaa tatggtttcc
    20701 ccgttcagcg gcaaacccat gcccctgccc tccggaagca aacattttct catttgtact
    20761 ccaggtttcc ggcagtttca tgagaatcag accattcgcc gttgcatcgg aaataaagct
    20821 gttgtaaggg cgttcataaa tttcctgaca cagtgtttct gtttcgcgac aaagagagga
    20881 gcagtaccat tccggatccg gcttatgatt ttccggggca tgatgtactt ctgtcagccc
    20941 gcaggaggca cagatacgaa gctcatccac gtggtggaat gatacaaaac ggcggccttc
    21001 tgaaaagggg taccagtcac cgccgtccag gcaacgatcg agaaaatcgt cattcgcttc
    21061 cggtgataaa ttcaggcggt tccggaagag cgttttcagt tcatcaggtg tgaatgacag
    21121 gttctctttc aactgttcgt taaaccctgc catgtttctt tcataccagg cattgttcag
    21181 taaccagttt ccgtctgcca gttcaaattc cctcagctct ccccgggaaa tccgttgaga
    21241 aatgagcgtc agccagatgc gctccgtcag tgagtcagga aggctgtaat cgttttgcaa
    21301 actggtcagc cacgatttac tgttgagcac cggttgttct tccagtgtct ggctgatggc
    21361 acaggtcagt tttttcagca cctggatctg gctggccgcc catagcagcg tcactaatgc
    21421 agccagccag tacaacaacc cgccccacca catcagtgag ggtgaaatat cgtactgtaa
    21481 cgcgatcaat ggtgcgcatg cagaacacgc cataaggaaa aaggcagtat aaaaacagca
    21541 gtaatacccg ttaatttcct tgctgatatc ttctggtgtg atcccgttct gctgaatgtt
    21601 tttgatctgc aggggaacag ccagcagacc accggcgaga gcagtggtca gaataaattt
    21661 tacagagtca aaggtgtgag cctccagcgc gatatgttta ccatcaagtc cccacatgaa
    21721 gctgatgatc attccggcaa tcgtcagaaa tacgcctgcc agccagcgaa tatggaacaa
    21781 ccgggttatg cgttcggtga tatagccagc cagtaaggcc gggccgaaca gacacagcaa
    21841 tatgagccct gccagcgcag aagccgtgct gattaatgct ctgaacaaaa ggcgaagaat
    21901 ggcaaatcct gcgatcagca ggatcagcgc gccgactatc tgtaacatag tcactccgtg
    21961 atgaatatca tgttattgtt aatgccagcg aatgtggcgt tgaagatccg ttgcgccggt
    22021 ccgggtcccg gtcatgatga ccgggaccac agagaggcga tggttctgtc agaaggtcac
    22081 attcagtgtg gcctgaccgt tatacccttc agcgctgctg ccgctgacgc tgtgggcata
    22141 accggcctga acgcccaggg tgatattttc ccggacacgg gcttccagtc cggcctgcag
    22201 gtccagtgat gtgccattct gtgagggaga gaacgtcatc ccgctgcctg cggtggaggt
    22261 ccccacacgc atatcacccc gggagctgaa ggtgcggata acggaaggct gtacccacca
    22321 gttcaccggc agttcacgca cactgtgttt tgcactgtca cgcagggggg cacgggatga
    22381 ggtgccttca ccaaaggtca tatcgttgtg gctgcccaga cggaaaccgg cacgcacatg
    22441 ttgtgcactg ccatgcccga acttcacata accggcgttg tccttaccgt catccagcga
    22501 aagcccctgc caggtgtact gcagccgtgg ctccagcatc aggttgtcag tgatactgaa
    22561 gggcagaccg gtttccagtg agcccagcca gccccggccc cgtgcgcgga agtcgttatt
    22621 gcccgatgac gctttcatac tgtggcgggt tccctgtgcc acaatgtcag cccacaggcc
    22681 ggaggaggtg tgtgtcaggt tcatatatcc gcccaggctg ccggcatcat cccggaccgt
    22741 gccggcgcgg gagccgtcat catccttaac atcaacggaa gaatggcctg cagcaccata
    22801 cactcctgtc gtcagtgaca taccggcgac ctctgttctg agcaggtcac tctccagacg
    22861 gacgaagcca tagctgccgc tgctttccgg cgtggctcca cgggcaatac cgccgttgtt
    22921 atcgtgaccg agatgaccgc cctgaatgct gagacggacg ctgttatttt caccgcttac
    22981 accggtctga tggctgcggg agcctgccag aatccggtca tagtccattg cctgtgtcag
    23041 catggaggca tacaggggga cttctgcacg ataagcattt tcactgcgca gataccagct
    23101 ctcatcactg tcccggttga gggagtagtt aaaggcaccg gcctgcagct tattcccctg
    23161 gataaaggcc ccttcctccg tggtggcacc gttaatggct tccaccacct gaataccctt
    23221 accgctggtc gccagccccg acgcactgtt gccggcgttc accaggttca ggatggtttt
    23281 tccggttgcc ctgccaccgt caatgaccag tctgtcagca ttgttctgcg ccatatccgg
    23341 gcgtacacgc aggctgatgg tgccattctg cccgttcagg tttttcactt tcagggttgt
    23401 cggaacgaac ttccctgtgc gggcggaggt gaaatgaatc tgtcctgcat ggctgaggtc
    23461 atccaccacc gactgaaccg tggcgttatc ggggatattc caggtggcac cggatgtcag
    23521 ggtgacattc gtggggtcaa tggcaccgtt cagcactgtg ctgccggtca gcttcagggc
    23581 cgtgccgcgc tgagcgatga catccgtggt gacggtactg ttgttcagcg tcagcgtgcc
    23641 ttcattcagg ttgacggctt tctgggtgag tgtggtgttg ctgacagtga gtgtgccact
    23701 tcctgatttt tccaccctgc cgttaccggt aagagcgcct ccctgcagga gtgcatcacc
    23761 ttcacggatg gtcagggtat cgttatttac cgtcttcccg gaaagggtaa gtatggcgcc
    23821 gttattcagc gtggtggtgc ccgccagtgt gccgcccctg gcggtgaaca gtccgccatt
    23881 taccgtggta tccgtggcca tatcaccggc gttcagcgtg aatgaactgc ctttttccag
    23941 catcagggca tccgcctgac cgcctccgat actgaatgcc tttccgtcgc tccgggtacc
    24001 actgacagcg gcaccggaat cggccagcag tacaccgcca tttcccatgg atacggtggt
    24061 ggcggtacca ccgttgcgga catccagcgt tccgccatca tccacgcggg tattagtggc
    24121 tgtgtgtccg gtcagcacat ccaggcgtcc gccattttcc agtacgacat tatcagcttt
    24181 accctccaca accgagaatg ctccaaggcg gtttatgccg gtaacggttg cagccgtact
    24241 ggtaaccagt gcgccgccct gcttcagggt gacattcgtg gctgtaccac cggcgtccac
    24301 ctgcagtctg cccttctgat taacggtggt attcccggcc acacccccgt ttttgacaat
    24361 ctgccagccg tcactgttca caacagtgtc agacgctgta ccgccgttgt gcacatactg
    24421 gtatccccca ttcagcgtgg tatccagtgc gtgaccatgt acagtctggt cgccgccggc
    24481 ataaaccaca gtggtatttg tcgttccttc agttctcaca atctggcgac cgtttttatt
    24541 gatggttgtg cgtacggcat ccccgcgaac aaactgcccg gtatcaccgt tttctgcatc
    24601 cggtccccct tccgccccgg tattaacaac ggtatccgtt gccactgtac cgggcttgac
    24661 gacctgccag cccttatcat taatgacggt tcctgtggct atcgccccct catgcatcca
    24721 ctgttcgcca ccattcagcg tggtgttcac tgcccgtccc tgaaggctct gtccgcctcc
    24781 ggcactgata accgtgtctg agacacttcc accggggttc actctctgaa gaccaccact
    24841 ggtgacagtc gttttgttgg ctgttccgcc atcctgtacc cattgcccgc cggtattggc
    24901 ctcgttatct ggcccatact ccagcccggt actgatggtc attccgttgg tcgtaccgaa
    24961 gacaatctgg ttgtcatgat ttgccagtgt tccgccgttc acggtttctc ccgggcgtac
    25021 aacgatgtca gcagccagtg ccgggactga catgacagca gcaagagaca gagcaaccgc
    25081 cacaccggcg cgttttcccc gtgagcgggc cagttcggaa gccaccacca gggtgcccgt
    25141 aatgtgattc cataccagcc tgtagctggt gttcagatgt cgtttcatca gcttttcctt
    25201 agattgaggg tgaataaaaa agccggtacc cacaaacgtg gataccggca ggtaaagggt
    25261 tacagtgccg gaggtccggc tgtgggagtt tctgaattgt ggttctgttg ttccctgaat
    25321 gtctttctgt acgtatctgt gcatgttact gacaaaggtt attattagct tatcgatcga
    25381 tataaacgat cgataaaata gatatcgatc gttttattct attattgagc tattgcacgg
    25441 acaagagaga cagtaacgaa aacagtgctt ttgtgagcag actcagagtc agggagggca
    25501 tcagtcacga caggttcaga agaaaatcca gttccatacc gcacgggcaa cagagaccac
    25561 cgtgtcacga acagcgcgca gggccgtacg gacaacagag gcaacacaga cgctctccgc
    25621 tgtgtcaaat atccggtcca ccgcaccggt aaactgttca cgggcctgag agcggacaga
    25681 ttccgtgcgc agctcgtcct gcagtcgggt catcagggga ctggcggcat ggtcgggaag
    25741 tgctgtcatg agcgcactga ccagcgtatc cagttcccag ccggtgcggg ccgatacggc
    25801 cacaaccgga tgtacgggcc ggaacagacg gaataccgcc tccgtttttt cgcgaatatt
    25861 ctgtgcctgt gcaggagaag gctgaatacc ggccatatcc cattcatggc agggctccgt
    25921 tttgtcggcc tgtgtcacca caaacagcac ctgctgatgt ccgcactgca ggatgtgtcg
    25981 ccagaaatac tcatccacag acagggcacg gtcatcggct ttaatcagcc acagtaccag
    26041 gtccagttct ggcagaatgt cacggtacag ggcttcatac tctgcatccc tgtcccggct
    26101 ctcgcccacc ccgggcaggt cagtgataac catgctgtga ccgtggccac tcagacggaa
    26161 gcgccgcact tcccgggtgc cagcgtgaac atcactgacc ggggtgacct ccccctgaaa
    26221 cagtgcatta cagagtgaag atttaccggc cccgctttta cccataatgc caatcacggg
    26281 ctcgtgacgg gtgagtttgc gcagatgttc caggatgtga cgggaaagcg agtaaggcag
    26341 ggaggagagc ggtttttcaa ttgcctcaat ggcatcagac ggattcatac atgtattccc
    26401 ggtgaaaaaa gacaaaaccc cgcacaccgg aaaggtggcg ggggatccac ggagataata
    26461 tctgtctgaa attgtttgga atcgatgggg atgttgagta ttaacatgaa ggagggcagg
    26521 agttatccgt tattgggtga tatccttcca tgaaaatacg cggttattgt taaacagcag
    26581 gctgattact ccgtaaagga gaaaaatatt catcttgcag cctgattatc aggtgtgttt
    26641 atgtttactg aggtgagtat ttttcagtat attgtcgggg cagtcagtgg gagatattaa
    26701 atttacgttt agagttatct tgcttctgct gtgtattttt ataagagtgc gcacgtaatg
    26761 atatctgttg ataccatgtt gttaagtgtt tcccttccgg gtcatgcgta cttgcaaaat
    26821 ggcatctgaa tcgatcacgg tttggccaaa ccgtgatcga atagagagga atacgcacgt
    26881 aatgattaaa tgcttttgtt acaccattct tccggcatga atatttatcc agttcccttt
    26941 tttatttgtg tagtgatact gtcggaatag cattcagtaa tgtgaagtgt cctgtaaaaa
    27001 aactttaata aatgtgtcat cctggtcagg aaatatctgc tgagatatta ttagttggtg
    27061 gtgtttttac tgagtaatag ctggctttta attcctgaac tgaaatactg tcgctcgtca
    27121 gggtttaatt aaaactggat gatgatgtga aaatgaaggc tggtttttgt gtttgtcgaa
    27181 gaagtgttat tttaatccat tctcatcatt ggctggcgat tatacagaag tatttgaaga
    27241 aaaaatgttg cattttatct gtgtcagcag ggaggaagta ttttattgat gtatcagtat
    27301 taaatggttt ttgttattta taaaaatgtc atcctgtcca tagaatattg tactcagcat
    27361 tcagtgcagg aactgtcatc ttctttattt tattcttctt caggagggca gttttctgaa
    27421 agcgttactc cggggaaact gtttatgttt attcccggca ggaaacccgt acctgcaata
    27481 ccctggatga tgctttttgc tggtacgttg ttgtgtgaaa attaatcaac acgtgtgttc
    27541 cggagagcgc ccgtagacgc acagattcat tctaacattc tttagcgaca aaataccgaa
    27601 tggttattat ttaatctgaa aatcgacaga aatttgtatc ctgtattgat tctgctgcta
    27661 tttttgctat tagtagcaat agttgcggta ctttatgttc tgtgtgttgt gaggtcatta
    27721 tgccatactt tcagtatcct gatgagtttc ccctgagctc gctgcctcca ctcattcgtg
    27781 atgccgttat tgaagctcag caaatcacgc aggctcccct ggggcttgtg gcagcatctg
    27841 ccctgggcgc tgtttcactt gtctgccaga atctgattga tgtctgcaga ctgaatacac
    27901 tgcgggggcc ggtttctctt ttcttactga cgcttgctga gtccggagaa cgaaaaaccg
    27961 cagtggacaa attattaatg gagcccctgt accagcagga gatgctgtta tattccagac
    28021 acaaaaatga actgacgaca tggaaaaata aggaagagct tttaaaggcg cagaaaaaag
    28081 cattgttgtc aaaactgaat aaagaactcc gcaaaggcgc ggatgagtca gaaacgctca
    28141 gacaactgga agcgcttcag aaaaacagag gggaaaaacc tgtaagatat aaattcattt
    28201 ttaatgatgc cacgacagca gcaattaaag atcagctttg tggtcagtgg cgttcagtgg
    28261 gtatcatgtc tgatgaagcc ggtataatat ttgatgggta taccctgtct gagctgcctt
    28321 ttattaacaa aatgtgggat ggctccgtat tgtcggtgga cagaaagaat gaacccgaac
    28381 agatgattga aaatgccaga atgacattat ccctcatggt acaacccggt ctttttgacc
    28441 gatatatgga gcgaaaaggt tctgttgcca gggacagtgg ttttcttgcc cgttgtctaa
    28501 tcagtaaacc tgccaccacg caggggaaac gctttattaa cggagctgtg accccgggag
    28561 gatcattaac ggcatttcat gaacgattaa tggaactggc cagaggcagt attgaaaaat
    28621 ccagtaagga tgaacgttat tgtctgcatt tttctcctga agcgcaaaaa atatttattg
    28681 atcactataa tgttctggag caggatttgt ctccttccgg acccttaagc cagtttcggg
    28741 ggcatgtttc aaaaaaaaca gaaaacattg cgagaattgc tgcgttattg cagtatttca
    28801 gtcagggtga agggaaaatc tctgccgata tcatgacgtc tgctgtcgta ataagttcgt
    28861 ggtacacaga tgaatataaa aaattatttg cgttacctga tgaatctgaa ctgcagctgc
    28921 aggacgcccg ggagctgctc gactggctca ttgaggagtg ccgcggagaa tgcccgcccc
    28981 gtgtcaggaa aaattatatt ctccagtgtg gcccgggccg gttccggaac aggaaaaagc
    29041 tgaacgtcct gctgaacatt cttgcatctc aactcaggct ctctgtcgtg caggaaggaa
    29101 aaaccaccta tgtcctgctt tccgaaataa acaatatcac gctgcctgaa ctgaatgcgt
    29161 gctacagcca gaaactgata agatggcatg agcaatcctt tggcttataa tgttccctgt
    29221 tgctgagacg gattattcag tggtggtatc gcagtatgcc cgtcaccccc gcttctgcat
    29281 gagggtgacg gaggaaaagt aagtgtgcgg aggcccggga gcgttatgaa tcaacaggcg
    29341 tataacaggt actcgtcaat catcagttcc agctcgcgct tttcccgttt gcgcgccaca
    29401 gcccagtttt caagctctgc atgtgattta tgctttaact cttcagtcca gggctgcatg
    29461 gagtgggcac acatcagatc ataccagcac agccatatca gcgtgtggta gagatcacga
    29521 tcgtcgaaac gccggagcag gctgttcagc ctgtttgcca gccggttttc acccagacga
    29581 tgtagctcac tgagtaaaac gccgcccaga ttaaaatgtg taagcagcat gatttcatgg
    29641 gtctgaatgc tggtcattaa cccttcctga atggcaggaa tgaacggtgg tgttcccggg
    29701 ttgacgacct gatagagtcc ctgtggtgcc actgagatga tggtgccttt ttctggcaga
    29761 aaaaagtggg ccagggtcag gcggtgataa ccatgaagat gcggagcccc cattccatga
    29821 gcgacgataa gcctcgtcac gtttgtcatc catttctgat gctggatgag cagcagctca
    29881 caccggtttt caagtttttg cataatattc tctcctttag cgcgcagcaa aatgcctccg
    29941 ggggccggat catgcctttg ctgcggttga acggataacg gtcaggaatt acggacggga
    30001 ctgtgtaata cgtgcctgca accaggcttc cacttcactt ttcagccatc gggagctacg
    30061 gcccagtttg atgggggcag gaaaggcacc atccttgatg agcttgtaaa accatttatc
    30121 ggtcaggccg gtcagctgag tgataaacga catgtcgacc atctggtcat ccatcagtga
    30181 aactggggta ttcatgaaaa tgttcctccg gatgttgtta atctcaaccg gcgttggtga
    30241 tgaaaacaac gcaccacctt ccccggcata acaaccgggg aaaaacgtaa aaaataatcg
    30301 ggaatgtgac cgtcaggaga taaacggggg gattgcggtg gctgagccgt accggtatgt
    30361 cgggtgattg ttgcgaaacg atgcttccgc acgcgcacgt gataaaggag ggaggacttt
    30421 cgtgagacaa ggggaaaact ggcgttctga taattgcaga gttgtttttg aaggcggttg
    30481 tggtgcatca gcccgtgacc tgtgggcagg cgacaggtat cactggtggg ataagactgt
    30541 ctgattggta ttataaatat ataagagagc tcaaacacat gagttcatat atgtgaaata
    30601 aatctgtgag tgtgatcagc agaccagtct acccagtagc aacagattgc gttgtactca
    30661 cagggttatc cctgataact ggcgtataac ctgcgtacat aaaacgtacc tgcaatgcac
    30721 ctgaacagag tgcattaagc atccgcctga gcggtaaacg tatccgatct ggtgagtgac
    30781 agtctgagag cattctcagt aatcgccccc ttgtatcctg gaaattggtt aagataacag
    30841 cgattagatt tggcaaggtg tatgcagcca gcagcttaac atctcagcta actggctggt
    30901 taccttcagg ttgtcgcaat ggcatcggac atccggtgtc ggataactaa acgtagcgtt
    30961 aaggcgatat t
//
LOCUS       DAHHTG010000052        30509 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.052, whole genome shotgun sequence.
ACCESSION   DAHHTG010000052 DAHHTG010000000
VERSION     DAHHTG010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 30509)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30509)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30509
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.052"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            <1..864
                     /gene="tuf"
                     /locus_tag="NBI74_002256"
     CDS             <1..864
                     /gene="tuf"
                     /locus_tag="NBI74_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HCD1932580.1"
                     /translation="ATDGPMPQTREHILLGRQVGVPYIIVFLNKCDMVDDEELLELVE
                     MEVRELLSQYDFPGDDTPIVRGSALKALEGDAEWEAKILELAGFLDSYIPEPERAIDK
                     PFLLPIEDVFSISGRGTVVTGRVERGIIKVGEEVEIVGIKETQKSTCTGVEMFRKLLD
                     EGRAGENVGVLLRGIKREEIERGQVLAKPGTIKPHTKFESEVYILSKDEGGRHTPFFK
                     GYRPQFYFRTTDVTGTIELPEGVEMVMPGDNIKMVVTLIHPIAMDDGLRFAIREGGRT
                     VGAGVVAKVLS"
     gene            1094..1477
                     /gene="secE"
                     /locus_tag="NBI74_002257"
     CDS             1094..1477
                     /gene="secE"
                     /locus_tag="NBI74_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007665874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HCD1932581.1"
                     /translation="MSANTEAQGSGRGLEAMKWVVVVALLLVAIVGNYLYRDIMLPLR
                     ALAVVILIAAAGGVALLTTKGKATVAFAREARTEVRKVIWPTRQETLHTTLIVAAVTA
                     VMSLILWGLDGILVRLVSFITGLRF"
     gene            1479..2024
                     /gene="nusG"
                     /locus_tag="NBI74_002258"
     CDS             1479..2024
                     /gene="nusG"
                     /locus_tag="NBI74_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005280494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HCD1932582.1"
                     /translation="MSEAPKKRWYVVQAFSGFEGRVATSLREHIKLHNMEELFGEVMV
                     PTEEVVEIRGGQRRKSERKFFPGYVLVQMVMNDASWHLVRSVPRVMGFIGGTSDRPAP
                     ISDKEVDAIMNRLQQVGDKPRPKTLFEPGEMVRVNDGPFADFNGVVEEVDYEKSRLKV
                     SVSIFGRATPVELDFSQVEKA"
     gene            2183..2611
                     /gene="rplK"
                     /locus_tag="NBI74_002259"
     CDS             2183..2611
                     /gene="rplK"
                     /locus_tag="NBI74_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006121495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HCD1932583.1"
                     /translation="MAKKVQAYVKLQVAAGMANPSPPVGPALGQQGVNIMEFCKAFNA
                     KTDSIEKGLPIPVVITVYADRSFTFVTKTPPAAVLLKKAAGIKSGSGKPNKDKVGKIS
                     RAQLQEIAQTKAADMTGADIEAMTRSIEGTARSMGLVVED"
     gene            2615..3319
                     /gene="rplA"
                     /locus_tag="NBI74_002260"
     CDS             2615..3319
                     /gene="rplA"
                     /locus_tag="NBI74_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HCD1932584.1"
                     /translation="MAKLTKRMRVIREKVDATKQYDINEAIALLKELATAKFVESVDV
                     AVNLGIDARKSDQNVRGATVLPHGTGRSVRVAVFTQGANAEAAKAAGAELVGMEDLAE
                     QIKKGEMNFDVVIASPDAMRVVGQLGQVLGPRGLMPNPKVGTVTPNVAEAVKNAKAGQ
                     IRYRNDKNGIIHTTIGKVDFDADKLKENLEALLVALKKAKPTQAKGVYIKKVSISTTM
                     GAGVAVDQAGLSASVN"
     gene            3611..4108
                     /gene="rplJ"
                     /locus_tag="NBI74_002261"
     CDS             3611..4108
                     /gene="rplJ"
                     /locus_tag="NBI74_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002438625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HCD1932585.1"
                     /translation="MALNLQDKQAIVAEVSEVAKGALSAVVADSRGVTVDKMTELRKA
                     GREAGVYMRVVRNTLLRRAVEGTPFECLKDAFVGPTLIAYSMEHPGAAARLFKEFAKA
                     NAKFEVKAAAFEGELIPASQIDRLATLPTYEEAIARLMATMKEASAGKLVRTLAAVRD
                     AKEAA"
     gene            4175..4540
                     /gene="rplL"
                     /locus_tag="NBI74_002262"
     CDS             4175..4540
                     /gene="rplL"
                     /locus_tag="NBI74_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HCD1932586.1"
                     /translation="MSITKDQIIEAVAAMSVMDVVELISAMEEKFGVSAAAAVAVAAG
                     PVEAAEEKTEFDVILKAAGANKVAVIKAVRGATGLGLKEAKDLVESAPAALKEGVSKD
                     DAEALKKALEEAGAEVEVK"
     gene            4860..8888
                     /gene="rpoB"
                     /locus_tag="NBI74_002263"
     CDS             4860..8888
                     /gene="rpoB"
                     /locus_tag="NBI74_002263"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004870488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HCD1932587.1"
                     /translation="MVYSYTEKKRIRKDFGKRPQVLDVPYLLSIQLDSFQKFIEQDPE
                     GQYGLEAAFRSVFPIQSYSGNSELQYVSYRLGEPVFDVQECQIRGVTYSAPLRVKLRL
                     VIYEREAPEGTVKDIKEQEVYMGEIPLMTDNGTFVINGTERVIVSQLHRSPGVFFDSD
                     KGKTHSSGKVLYNARIIPYRGSWLDFEFDPKDNLFVRIDRRRKLPATIILRALNYTTE
                     QILDLFFEKVIFEIRDNKLQMELVPERLRGETASFDIEANGKVYVEKGRRITARHIRQ
                     LEKDDVKLIEVPVEYIAGKVVAKDYIDESTGELICAANMELSLDLLAKLSQSGHKRIE
                     TLFTNDLDHGPYISETLRVDPTNDRLSALVEIYRMMRPGEPPTREAAESLFENLFFSE
                     DRYDLSAVGRMKFNRSLLREEIEGSGILSKDDIIDVMKKLIDIRNGKGEVDDIDHLGN
                     RRIRSVGEMAENQFRVGLVRVERAVKERLSLGDLDTLMPQDMINAKPISAAVKEFFGS
                     SQLSQFMDQNNPLSEITHKRRISALGPGGLTRERAGFEVRDVHPTHYGRVCPIETPEG
                     PNIGLINSLSVYAQTNEYGFLETPYRKVTDGVVTDEIHYLSAIEEGNYVIAQANSNLD
                     EEGHFVEDLVTCRSKGESSLFSRDQVDYMDVSTQQVVSVGASLIPFLEHDDANRALMG
                     ANMQRQAVPTLRADKPLVGTGMERAVAVDSGVTAVAKRGGVVQYVDASRIVIKVNEDE
                     MYPGEAGIDIYNLTKYTRSNQNTCINQMPCVSLGEPVERGDVLADGPSTDLGELALGQ
                     NMRVAFMPWNGYNFEDSILVSERVVQEDRFTTIHIQELACVSRDTKLGPEEITADIPN
                     VGEAALSKLDESGIVYIGAEVTGGDILVGKVTPKGETQLTPEEKLLRAIFGEKASDVK
                     DSSLRVPNGVSGTVIDVQVFTRDGVEKDKRALEIEEMQLKQAKKDLSEELQILEAGLF
                     SRIRAVLVAGGVEAEKLDKLPRDRWLELGLTDEEKQNQLEQLAEQYDELKHEFEKKLE
                     AKRRKITQGDDLAPGVLKIVKVYLAVKRRIQPGDKMAGRHGNKGVISKINPIEDMPYD
                     ENGTPVDIVLNPLGVPSRMNIGQILETHLGMAAKGIGDKINAMLKQQQEVAKLREFIQ
                     RAYDLGADVRQKVDLSTFSDEEVMRLAENLRKGMPIATPVFDGAKEAEIKELLKLGDL
                     PTSGQIRLYDGRTGEQFERPVTVGYMYMLKLNHLVDDKMHARSTGSYSLVTQQPLGGK
                     AQFGGQRFGEMEVWALEAYGAAYTLQEMLTVKSDDVNGRTKMYKNIVDGNHQMEPGMP
                     ESFNVLLKEIRSLGINIELEDE"
     gene            8965..13188
                     /gene="rpoC"
                     /locus_tag="NBI74_002264"
     CDS             8965..13188
                     /gene="rpoC"
                     /locus_tag="NBI74_002264"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HCD1932588.1"
                     /translation="MKDLLKFLKAQTKTEEFDAIKIALASPDMIRSWSFGEVKKPETI
                     NYRTFKPERDGLFCARIFGPVKDYECLCGKYKRLKHRGVICEKCGVEVTQTKVRRERM
                     GHIELASPTAHIWFLKSLPSRIGLLLDMPLRDIERVLYFESYVVIEGGMTNLERQQIL
                     TEEQYLDALEEFGDEFDAKMGAEAIQALLKSMDLEQECEQLREELNETNSETKRKKLT
                     KRIKLLEAFVQSGNKPEWMILTVLPVLPPDLRPLVPLDGGRFATSDLNDLYRRVINRN
                     NRLKRLLDLAAPDIIVRNEKRMLQEAVDALLDNGRRGRAITGSNKRPLKSLADMIKGK
                     QGRFRQNLLGKRVDYSGRSVITVGPYLRLHQCGLPKKMALELFKPFIYGKLELRGLAT
                     TIKAAKKMVEREEAVVWDILDEVIREHPVLLNRAPTLHRLGIQAFEPVLIEGKAIQLH
                     PLVCAAYNADFDGDQMAVHVPLTLEAQLEARALMMSTNNILSPANGEPIIVPSQDVVL
                     GLYYMTRDCVNAKGEGMVLTGPKEAERLYRSGLASLHARVKVRITEYEKDANGELVAK
                     TSLKDTTVGRAILWMIVPKGLPYTIVNQALGKKAISKMLNTCYRILGLKPTVIFADQI
                     MYTGFAYAARSGASVGIDDMVIPEKKHEIISEAEAEVAEIQEQFQSGLVTAGERYNKV
                     IDIWAAANDRVSKAMMDNLQTETVINRDGQEEKQVSFNSIYMMADSGARGSAAQIRQL
                     AGMRGLMAKPDGSIIETPITANFREGLNVLQYFISTHGARKGLADTALKTANSGYLTR
                     RLVDVAQDLVVTEDDCGTHEGIMMTPVIEGGDVKEPLRDRVLGRVTAEDVLKPGTADI
                     LVPRNTLLHEQWCDLLEENSVDAVKVRSVVSCDTDFGVCAHCYGRDLARGHIINKGEA
                     IGVIAAQSIGEPGTQLTMRTFHIGGAASRAAAESSIQVKNKGSIKLSNVKSVVNSSGK
                     LVITSRNTELKLIDEFGRTKESYKVPYGAVLAKGDGEQVAGGETVANWDPHTMPVITE
                     VSGFVRFTDMIDGQTITRQTDELTGLSSLVVLDSAERTAGGKDLRPALKIVDAQGNDV
                     LIPGTDMPAQYFLPGKAIVQLEDGVQISSGDTLARIPQESGGTKDITGGLPRVADLFE
                     ARRPKEPAILAEISGIVSFGKETKGKRRLVITPVDGSDPYEEMIPKWRQLNVFEGERV
                     ERGDVISDGPEAPHDILRLRGVHAVTRYIVNEVQDVYRLQGVKINDKHIEVIVRQMLR
                     KATIVNAGSSDFLEGEQVEYSRVKIANRELEANGKVGATYSRDLLGITKASLATESFI
                     SAASFQETTRVLTEAAVAGKRDELRGLKENVIVGRLIPAGTGYAYHQDRMRRRAAGEA
                     PAAPQVTAEDASASLAELLNAGLGGSDNE"
     gene            complement(13430..14563)
                     /gene="thiH"
                     /locus_tag="NBI74_002265"
     CDS             complement(13430..14563)
                     /gene="thiH"
                     /locus_tag="NBI74_002265"
                     /EC_number="4.1.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000847471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminoacetate synthase ThiH"
                     /protein_id="HCD1932589.1"
                     /translation="MKTFSDRWRQLDWDDIHLRINGKTAADVERALNASQLTRDDMMA
                     LLSPAASGYLEPLAQRAQRLTRQRFGNVVSFYVPLYLSNLCANDCTYCGFSMSNRIKR
                     KTLDEADIARESAAIREMGFEHLLLVTGEHQAKVGMDYFRRHLPALREQFSSLQMEVQ
                     PLAEAEYAELKQLGLDGVMVYQETYHEATYAHHHLKGKKQDFFWRLETPDRLGRAGID
                     KIGLGALIGLSDSWRVDCYMVAEHLLWLQQHYWQSRYSVSFPRLRPCTGGIEPASIMD
                     ERQLVQAICAFRLLAPEIELSLSTRESPWFRDRVIPLAINNVSAFSKTQPGGYADNHP
                     ELEQFSPHDDRRPEAVAAALTAQGLQPVWKDWDSYLGRPSQRP"
     gene            complement(14560..15330)
                     /gene="thiG"
                     /locus_tag="NBI74_002266"
     CDS             complement(14560..15330)
                     /gene="thiG"
                     /locus_tag="NBI74_002266"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HCD1932590.1"
                     /translation="MLHIADKTFDSHLFTGTGKFASSQLMVESIRASGSQLVTLAMKR
                     VDLRQHNDAILEPLIAAGVALLPNTSGAKTAEEAIFAAHLAREALGTNWLKLEIHPDA
                     RWLLPDPIETLKAAETLVQQGFVVLPYCGADPVLCKRLEEVGCAAVMPLGAPIGSNQG
                     LETRAMLEIIIQQATVPVVVDAGIGVPSHAAQALEMGADAVLVNTAIAVADDPVNMAK
                     AFRLAVEAGLLARQSGPGSRSHFAHATSPLTGFLEASA"
     gene            complement(15332..15532)
                     /gene="thiS"
                     /locus_tag="NBI74_002267"
     CDS             complement(15332..15532)
                     /gene="thiS"
                     /locus_tag="NBI74_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HCD1932591.1"
                     /translation="MQILFNDQPMQCVAELTVHELLEQLEQQQAGAALAINQQIVPRE
                     QWAQHIVQDGDQILLFQVIAGG"
     gene            complement(15516..16271)
                     /gene="thiF"
                     /locus_tag="NBI74_002268"
     CDS             complement(15516..16271)
                     /gene="thiF"
                     /locus_tag="NBI74_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418420.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole biosynthesis adenylyltransferase ThiF"
                     /protein_id="HCD1932592.1"
                     /translation="MNDRDFMRYSRQILLDDIALDGQQKLLDSQVLIIGLGGLGTPAA
                     LYLAGAGVGTLVLADDDDVHLSNLQRQILFTTEDIDRPKSQVSQQRLTQLNPDIRLMA
                     LQQRLTGETLKDAVAQADVVLDCTDNMATRQEINATCVALNTPLITASAVGFGGQLMV
                     LTPPWEQGCYRCLWPDNQEPERNCRTAGVVGPVVGVMGTLQALEAIKLLSGIETPAGE
                     LRLFDGKSSQWRSLTLRRASGCPVCGGCNADPV"
     gene            complement(16264..16899)
                     /gene="thiE"
                     /locus_tag="NBI74_002269"
     CDS             complement(16264..16899)
                     /gene="thiE"
                     /locus_tag="NBI74_002269"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HCD1932593.1"
                     /translation="MYQPEFPPVPFRLGLYPVVDSVQWIERLLDAGVRTLQLRIKDQR
                     DEEVEADVVAAIALGRRYNARLFINDYWRLAIKHQAYGVHLGQEDLQATDLNAIRAAG
                     LRLGVSTHDDMEIDVALAARPSYIALGHVFPTQTKQMPSAPQGLEQLARHVERLADYP
                     TVAIGGISLARAPAVIATGVGSIAVVSAITQAADWRLATAQLLEIAGVGDE"
     gene            complement(16899..18794)
                     /gene="thiC"
                     /locus_tag="NBI74_002270"
     CDS             complement(16899..18794)
                     /gene="thiC"
                     /locus_tag="NBI74_002270"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HCD1932594.1"
                     /translation="MSATKLTRREQRAQAQHFIDTLEGSAFPNSKRIYITGTHPGVRV
                     PMREIQLSPTLIGGSKEQPQYEENEAIPVYDTSGPYGDPQIAINVQQGLAKLRQPWID
                     ARGDTEELTVRSSDYTKARLADDGLDELRFSGVLTPKRAKAGRRVTQLHYARKGIITP
                     EMEFIAIRENMGRERIRSEVLRHQHPGMSFGARLPENITAEFVRDEVAAGRAIIPANI
                     NHPESEPMIIGRNFLVKVNANIGNSAVTSSIEEEVEKLVWSTRWGADTVMDLSTGRYI
                     HETREWILRNSPVPIGTVPIYQALEKVNGIAEDLTWEVFRDTLLEQAEQGVDYFTIHA
                     GVLLRYVPMTAKRLTGIVSRGGSIMAKWCLSHHQENFLYQHFREICEICAAYDVSLSL
                     GDGLRPGSIQDANDEAQFAELHTLGELTKIAWEYDVQVMIEGPGHVPMQMIRRNMTEE
                     LEHCHEAPFYTLGPLTTDIAPGYDHFTSGIGAAMIGWFGCAMLCYVTPKEHLGLPNKE
                     DVKQGLITYKIAAHAADLAKGHPGAQIRDNAMSKARFEFRWEDQFNLALDPFTARAYH
                     DETLPQESGKVAHFCSMCGPKFCSMKISQEVRDYAAAQTIEVGMADMSENFRARGGEI
                     YLRKEEA"
     regulatory      complement(18875..18975)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(19027..19503)
                     /gene="rsd"
                     /locus_tag="NBI74_002271"
     CDS             complement(19027..19503)
                     /gene="rsd"
                     /locus_tag="NBI74_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma D regulator"
                     /protein_id="HCD1932595.1"
                     /translation="MLNQLDNLTERVRGSNKLVDRWLHVRKHLLVAYYNLVGIKPGKE
                     SYMRLNEKALDDFCQSLVDYLSAGHFSIYERILHKLEGNGQLARAAKIWPQLEANTQQ
                     IMDDYDSSLETAIDHDNYLEFQQVLSDIGEALEARFVLEDKLILLVLDAARVKYPA"
     gene            19598..20371
                     /gene="nudC"
                     /locus_tag="NBI74_002272"
     CDS             19598..20371
                     /gene="nudC"
                     /locus_tag="NBI74_002272"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HCD1932596.1"
                     /translation="MDRIIEKLDHGWWVVSHEQKLWLPKGELPYGEAANFDLVGQRAL
                     QIGEWQGEPVWLVQQQRRHDMGSVRQVIDLDVGLFQLAGRGVQLAEFYRSHKYCGYCG
                     HEMYPSKTEWAMLCSHCRERYYPQIAPCIIVAIRRDDSILLAQHTRHRNGVHTVLAGF
                     VEVGETLEQAVAREVMEESGIKVKNLRYVTSQPWPFPQSLMTAFMAEYDSGEIVIDPK
                     ELLEANWYRYDDLPLLPPPGTVARRLIEDTVAMCRAEYE"
     gene            20411..21475
                     /gene="hemE"
                     /locus_tag="NBI74_002273"
     CDS             20411..21475
                     /gene="hemE"
                     /locus_tag="NBI74_002273"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HCD1932597.1"
                     /translation="MTELKNDRYLRALLRQPVDVTPVWMMRQAGRYLPEYKATRAQAG
                     DFMSLCKNAELACEVTLQPLRRYPLDAAILFSDILTVPDAMGLGLYFEAGEGPRFTSP
                     VTCKADVDKLPIPDPEDELGYVMNAVRTIRRELKGEVPLIGFSGSPWTLATYMVEGGS
                     SKAFTVIKKMMYADPQALHALLDKLAKSVTLYLNAQIKAGAQAVMIFDTWGGVLTGRD
                     YQQFSLYYMHKIVDGLLRENDGRRVPVTLFTKGGGQWLEAMAETGCDALGLDWTTDIA
                     DARRRVGNKVALQGNMDPSMLYAPPARIEEEVASILAGFGHGEGHVFNLGHGIHQDVP
                     PEHAGVFVEAVHRLSEQYHR"
     gene            21485..22156
                     /gene="nfi"
                     /locus_tag="NBI74_002274"
     CDS             21485..22156
                     /gene="nfi"
                     /locus_tag="NBI74_002274"
                     /EC_number="3.1.21.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease V"
                     /protein_id="HCD1932598.1"
                     /translation="MDLASLRAQQIELASSVIREDRLDKDPPDLIAGADVGFEQGGEV
                     TRAAMVLLKYPSLELVEYKVARIATTMPYIPGFLSFREYPALLAAWEMLSQKPDLVFV
                     DGHGISHPRRLGVASHFGLLVDVPTIGVAKKRLCGKFEPLSSEPGALAPLMDKGEQLA
                     WVWRSKARCNPLFIATGHRVSVDSALEWVQRCMKGYRLPEPTRWADAVASERPAFVRY
                     TANQP"
     gene            22199..22789
                     /locus_tag="NBI74_002275"
     CDS             22199..22789
                     /locus_tag="NBI74_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjaG family protein"
                     /protein_id="HCD1932599.1"
                     /translation="MLQNPIHLRLERLASWQHVTFMACLCERMYPNYAVFCQQTGFGD
                     GQIYRRILDLIWETLTVKDAKVNFDSQLEKFEEAIPSADDFDLYGVYPAIDACVALSE
                     LVHSRLSGETLEHAVEVSKTSITTVAMLEMTQAGREMSDEELKENPAVEQEWDIQWEI
                     FRLLAECEERDIELIKGLRADLREAGESNIGIIFQQ"
     gene            22976..23248
                     /gene="hupA"
                     /locus_tag="NBI74_002276"
     CDS             22976..23248
                     /gene="hupA"
                     /locus_tag="NBI74_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017460010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein HU-alpha"
                     /protein_id="HCD1932600.1"
                     /translation="MNKTQLIDVIAEKAELSKTQAKAALESTLAAITESLKEGDAVQL
                     VGFGTFKVNHRAERTGRNPQTGKEIKIAAANVPAFVSGKALKDAVK"
     gene            23261..23956
                     /locus_tag="NBI74_002277"
     CDS             23261..23956
                     /locus_tag="NBI74_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1481 domain-containing protein"
                     /protein_id="HCD1932601.1"
                     /translation="MNSFNEGVVSPLLSFWRRSLMLAGALFLTACSHNSSLPPFTASG
                     FAEDQGAVRIWRKDSGDNVHLLAVFSPWRSGDTTTREYRWQGDNLTLININVYSKPPV
                     NIRARFDDRGDLSFMQRESDGQKQQLSNDQIDLYRYRAAQIREISDALRQGRVVLRQG
                     RWHAMEQTVTTCEGQTIKPDLDSQAIAHIERRQSRSSVDVSVAWLEAPEGSQLLLVAN
                     SDFCRWQPNEKTF"
     gene            complement(23958..24377)
                     /gene="zraP"
                     /locus_tag="NBI74_002278"
     CDS             complement(23958..24377)
                     /gene="zraP"
                     /locus_tag="NBI74_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc resistance sensor/chaperone ZraP"
                     /protein_id="HCD1932602.1"
                     /translation="MKRNTKIALVMMALSAMAMGSTSAFAHGGHGMWQQNAAPLTSEQ
                     QTAWQKIHNDFYAQSSALQQQLVTKRYEYNALLAANPPDSSKINAVAKEMENLRQSLD
                     ELRVKRDIAMAEAGIPRGTGMGYGGCGGGGHMGMGHW"
     gene            24615..25991
                     /gene="zraS"
                     /locus_tag="NBI74_002279"
     CDS             24615..25991
                     /gene="zraS"
                     /locus_tag="NBI74_002279"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     ZraS"
                     /protein_id="HCD1932603.1"
                     /translation="MRFMQRSKDSLAKWLSAILPVVIVGLVGLFAVTVIRDYGRETAA
                     ARQTLLEKGSVLIRALESGSRVGMGMRMHHAQQQALLEEMAGQPGVRWFAVTDEQGTI
                     VMHSNSGMVGKQLYSPQEMQQLHPGDEEAWRRIDSADGEPVLEIYRQFQPMFAAGMHR
                     MRHMQQYAATPQAIFIAFDASNIVSAEDREQRNTLIILFALATVLLASVLSFFWYRRY
                     LRSGQLLQDEMKRKEKLVALGHLAAGVAHEIRNPLSSIKGLAKYFAERAPAGGEAHQL
                     AQVMAKEADRLNRVVSELLELVKPTHLALQAVELNTLISHSLQLVSQDANSREIQLRF
                     TANDTLPEIQADPDRLTQVLLNLYLNAIQAIGLHGVISVTASESGAGVKISVTDSGKG
                     IAADQLEAIFTPYFTTKAEGTGLGLAVVHNIVEQHGGTIQVASQEGKGATFTLWLPVN
                     ITRKDPQG"
     gene            25988..27313
                     /gene="zraR"
                     /locus_tag="NBI74_002280"
     CDS             25988..27313
                     /gene="zraR"
                     /locus_tag="NBI74_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent response regulator
                     transcription factor ZraR"
                     /protein_id="HCD1932604.1"
                     /translation="MTHDNIDILVVDDDISHCTILQALLRGWGYNVALANSGRQALEQ
                     VREQVFDLVLCDVRMAEMDGIATLKEIKALNPAIPVLIMTAYSSVETAVEALKTGALD
                     YLIKPLDFDNLQATLEKALAHTHSIDAETPAVSASQFGMVGKSPAMQHLLSEIALVAP
                     SEATVLIHGDSGTGKELVARAIHASSARSEKPLVTLNCAALNESLLESELFGHEKGAF
                     TGADKRREGRFVEADGGTLFLDEIGDISPMMQVRLLRAIQEREVQRVGSNQTISVDVR
                     LIAATHRDLAAEVNAGRFRQDLYYRLNVVAIEVPSLRQRREDIPLLAGHFLQRFAERN
                     RKAVKGFTPQAMDLLIHYDWPGNIRELENAVERAVVLLTGEYISERELPLAIASTPIP
                     LVQSQDIQPLVEVEKEVILAALEKTGGNKTEAARQLGITRKTLLAKLSR"
     gene            complement(27310..28599)
                     /gene="purD"
                     /locus_tag="NBI74_002281"
     CDS             complement(27310..28599)
                     /gene="purD"
                     /locus_tag="NBI74_002281"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HCD1932605.1"
                     /translation="MKVLVIGNGGREHALAWKAAQSPLVETVFVAPGNAGTALEPALQ
                     NVAIGVTDIPALLDFAQNEKVDLTIVGPEAPLVKGVVDTFRAAGLKIFGPTAGAAQLE
                     GSKAFTKDFLARHNIPTAEYQNFTEVEPALAYLREKGAPIVIKADGLAAGKGVIVAMT
                     LEEAEAAVHDMLAGNAFGDAGHRIVIEEFLDGEEASFIVMVDGEHVLPMATSQDHKRV
                     GDKDTGPNTGGMGAYSPAPVVTDEVHQRTMERIIWPTVKGMAAEGNTYTGFLYAGLMI
                     DKQGNPKVIEFNCRFGDPETQPIMLRMKSDLVELCLAACEGKLDEKTSEWDERASLGV
                     VMAAGGYPGDYRTGDVIHGLPLEEVADGKVFHAGTKLADDEQVVTSGGRVLCVTALGH
                     TVAEAQKRAYALMTDIHWDDCFCRKDIGWRAIEREQN"
     gene            complement(28611..30200)
                     /gene="purH"
                     /locus_tag="NBI74_002282"
     CDS             complement(28611..30200)
                     /gene="purH"
                     /locus_tag="NBI74_002282"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HCD1932606.1"
                     /translation="MQQRRPVRRALLSVSDKAGIVEFAQALSARGVELLSTGGTARLL
                     AEKCLPVTEVSDYTGFPEMMDGRVKTLHPKVHGGILGRRGQDDAIMEEHQIQPIDMVV
                     VNLYPFAQTVAREGCSLEDAVENIDIGGPTMVRSAAKNHKDVAIVVKSSDYDAIIKEM
                     DANEGSLTLATRFDLAIKAFEHTAAYDSMIANYFGSMVPAYHGESKEAAGRFPRTLNL
                     NFIKKQDMRYGENSHQQAAFYIEENVKEASVATATQVQGKALSYNNIADTDAALECVK
                     EFAEPACVIVKHANPCGVAIGNSILDAYDRAYKTDPTSAFGGIIAFNRELDAETAQAI
                     ISRQFVEVIIAPSASEEALKITAAKQNVRVLTCGQWGERIPGLDFKRVNGGLLVQDRD
                     LGMVGAEELRVVTKRQPTEQELRDALFCWKVAKFVKSNAIVYAKNNMTIGIGAGQMSR
                     VYSAKIAGIKAADEGLEVKGSSMASDAFFPFRDGIDAAAAAGVTCVIQPGGSIRDDEV
                     IAAADEHGIAMLFTDMRHFRH"
BASE COUNT         7140 a         7903 c         8420 g         7046 t
ORIGIN      
        1 gcgactgacg gcccgatgcc gcagactcgt gagcacatcc tgctgggtcg tcaggtaggc
       61 gttccgtaca tcatcgtgtt cctgaacaaa tgcgacatgg ttgatgacga agagctgctg
      121 gaactggttg aaatggaagt tcgtgaactt ctgtctcagt acgacttccc gggcgacgac
      181 actccgatcg ttcgtggttc tgctctgaaa gcgctggaag gcgacgcaga gtgggaagcg
      241 aaaatcctgg aactggctgg cttcctggat tcttacattc cggaaccaga gcgtgcgatt
      301 gacaagccgt tcctgctgcc gatcgaagac gtattctcca tctccggtcg tggtaccgtt
      361 gttaccggtc gtgtagaacg cggtatcatc aaagttggtg aagaagttga aatcgttggt
      421 atcaaagaga ctcagaagtc tacctgtact ggcgttgaaa tgttccgcaa actgctggac
      481 gaaggccgtg ctggtgagaa cgtaggtgtt ctgctgcgtg gtatcaaacg tgaagaaatc
      541 gaacgtggtc aggtactggc taagccgggc accatcaagc cgcacaccaa gttcgaatct
      601 gaagtgtaca ttctgtccaa agatgaaggc ggtcgtcata ctccgttctt caaaggctac
      661 cgtccgcagt tctacttccg tactactgac gtgactggta ccatcgaact gccggaaggt
      721 gtagagatgg taatgccggg cgacaacatc aaaatggttg ttaccctgat ccacccgatc
      781 gcgatggacg acggtctgcg tttcgcaatc cgtgaaggcg gccgtaccgt tggcgcgggc
      841 gttgtagcaa aagttctgag ctaattgccg ataacatttg acgcaatgcg cactaaaagg
      901 gcatcatttg atgccctttt tgcacgcttt cgtaccagaa cctggctcat cagtgatttt
      961 ctttgtcata atcattgctg agacaggctc tgttgagggc gtataatccg aaaagctaat
     1021 acgcgtttcg atttggtttg cctcgcgatc gcggggtgaa aatgtttgta gaatacttct
     1081 gacaggttgg tttatgagtg cgaataccga agctcaagga agcgggcgcg gcctggaagc
     1141 gatgaagtgg gtcgttgtgg tggcattgct cctggtagcg attgtcggca actaccttta
     1201 tcgcgacatt atgctgccgc tgcgtgcgct ggccgtagta attctgattg ctgcagcggg
     1261 tggtgtcgcg ctgttaacga caaaaggtaa agcaaccgtt gcttttgccc gtgaagcgcg
     1321 taccgaagtc cgtaaggtca tttggccgac tcgccaggaa acattgcaca ccacgctgat
     1381 tgtggctgcg gttaccgcag taatgtcact gatcctgtgg ggactggatg gtattctggt
     1441 tcgcctggta tcctttatca ctggcctgag gttctgagat gtctgaagct cctaaaaagc
     1501 gctggtacgt cgttcaggcg ttttccggtt ttgaaggccg cgtagcaacg tcgctgcgtg
     1561 agcatatcaa attacacaac atggaagagt tgtttggtga agtcatggta ccaaccgaag
     1621 aagtggttga aatccgtggc ggtcagcgtc gcaaaagcga acgtaaattc ttccctggct
     1681 acgtcctcgt tcagatggtg atgaacgacg cgagctggca cctggtgcgc agcgtaccgc
     1741 gtgtgatggg cttcatcggc ggtacttccg atcgtcctgc gccaatcagc gataaagaag
     1801 tcgatgcgat tatgaaccgc ctgcagcagg ttggtgataa gccgcgtccg aaaacgttgt
     1861 ttgaaccggg tgaaatggtc cgtgttaatg atggtccgtt cgctgacttc aacggcgttg
     1921 ttgaagaagt tgattacgag aaatctcgtc tgaaagtgtc tgtttctatc ttcggtcgtg
     1981 cgacccctgt agagctggac ttcagccagg ttgaaaaagc ttaacccagc gatcaaaaaa
     2041 gcggcgattt aatcgttgca caaggcgcga gattggaata caatttcgcg ccttttgttt
     2101 ttatgggcct tgcccgtaaa acgatttttt atatcacggg gagcctctca gaggcgttat
     2161 tacccaactt gaggaattta taatggctaa gaaagtacaa gcctatgtca agctgcaggt
     2221 tgcagctggt atggctaacc cgagtccgcc agtaggtccg gctctgggtc agcagggcgt
     2281 aaacatcatg gaattctgca aagcgttcaa cgcaaaaact gattccatcg aaaaaggtct
     2341 gccgattccg gtagtaatca ctgtttacgc tgaccgttct ttcactttcg ttaccaagac
     2401 tccgccggca gcagttctgc tgaaaaaagc ggctggtatc aagtctggtt ccggtaagcc
     2461 gaacaaagac aaagtgggta aaatttcccg cgctcaactg caggaaatcg cgcagaccaa
     2521 agctgccgac atgactggtg ccgacattga agcgatgact cgctccatcg aaggtactgc
     2581 acgttccatg ggcctggtag tggaggacta agaaatggct aaactgacca agcgcatgcg
     2641 tgttatccgc gagaaagttg atgcaaccaa acagtacgac atcaacgaag ctatcgctct
     2701 gctgaaagag ctggcgactg ctaaattcgt agaaagcgtg gacgtagctg ttaacctcgg
     2761 catcgacgct cgtaaatctg accagaacgt acgtggtgca actgtactgc cgcacggtac
     2821 tggccgttcc gttcgcgtag ccgtatttac ccagggtgca aatgctgaag ctgctaaagc
     2881 tgcaggtgct gaactggtag gtatggaaga tctggctgaa cagatcaaga aaggcgaaat
     2941 gaactttgac gttgttattg cttctccgga tgcaatgcgc gttgttggcc agctgggcca
     3001 ggttctgggt ccgcgcggcc tgatgccaaa cccgaaagtg ggtactgtaa cgccgaacgt
     3061 tgctgaagcg gttaaaaacg ctaaagctgg ccagattcgt taccgtaacg acaaaaacgg
     3121 catcatccac accaccatcg gtaaagtaga ctttgacgct gacaaactga aagaaaacct
     3181 ggaagctctg ctggttgcgc tgaaaaaagc aaaaccgact caggcgaaag gcgtgtacat
     3241 caagaaagtt agcatctcta ccaccatggg tgcaggtgtt gcagttgacc aggctggcct
     3301 gagcgcttct gtaaactaat gcctttacgt gggcggtgat tttgtctaca atcttacccc
     3361 cacgtacgct aacgaaagtt agcagctaaa aagatttgtt cgttggagcc tggcctatct
     3421 aggcctccgt cgaagaccgc aggagtttcg caagaaactt aatcccctgc gtagacggtg
     3481 acagaacgct aagattattc ttttatattc tggcttgttt ctgctcaccg taattaagac
     3541 gctctctccg tttggaggag tgaagtgagt tccagagatt ttctctggca aacatccagg
     3601 agcaaagcta atggctttaa atcttcaaga caaacaagcg attgttgctg aagtcagcga
     3661 agtagccaaa ggcgcgctgt ctgcagtagt tgcggattcc cgtggcgtaa ctgtagataa
     3721 aatgactgaa ctgcgtaaag caggtcgcga agctggcgta tacatgcgtg ttgttcgtaa
     3781 caccctgctg cgccgtgctg ttgaaggtac tccgttcgag tgcctgaaag acgcgtttgt
     3841 tggtccgacc ctgattgcat actctatgga acacccgggc gctgctgctc gtctgttcaa
     3901 agagttcgcg aaagcgaatg caaaatttga ggtcaaagcc gctgcctttg aaggtgagct
     3961 gatcccggcg tctcagatcg accgcctggc aactctgccg acctacgaag aagcaattgc
     4021 acgcctgatg gcaaccatga aagaagcttc ggctggcaaa ctggttcgta ctctggctgc
     4081 tgtacgcgat gcgaaagaag ctgcttaatc gcagttatct ttttaacgca ttcgcttacg
     4141 tataaactta ttctgatatt caggaacaat ttaaatgtct atcactaaag atcaaatcat
     4201 tgaagcagtt gcagctatgt ctgtaatgga cgttgtagaa ctgatctctg caatggaaga
     4261 aaaattcggt gtttccgctg ctgctgctgt agctgtagct gctggcccgg ttgaagctgc
     4321 tgaagaaaaa actgaattcg acgtaattct gaaagctgct ggcgctaaca aagttgctgt
     4381 tatcaaagca gtacgtggcg caactggcct gggtctgaaa gaagctaaag acctggttga
     4441 atctgcaccg gctgctctga aagaaggcgt gagcaaagac gacgcagaag cactgaaaaa
     4501 agctctggaa gaagctggcg ctgaagttga agttaaataa gccaaccctt ccggttgcag
     4561 cctgagaaat caggctgatg gctggtgact ttttagtcac cagccttttt gcgctgtaag
     4621 gcgccagtag cgtttcacac tgtttgacta ctgctgtgcc tttcaatgct tgtttctatc
     4681 gacgacttaa tatactgcga caggacgccc gttctgtgta aatcgcaatg aaatggttta
     4741 agcgtgatag caacaggcat tgcggaaagt gttccatttt ccggtcaaca aaatagtgtt
     4801 gcacaaactg tccgctcaat ggacagatgg gtcgacttgt cagcgagctg aggaacccta
     4861 tggtttactc ctataccgag aaaaaacgta ttcgtaagga ttttggtaaa cgtccacaag
     4921 ttctggatgt accttatctc ctttctatcc agcttgactc gtttcagaaa tttatcgagc
     4981 aagatcctga agggcagtat ggtctggaag ctgctttccg ttccgtattc ccgattcaga
     5041 gctacagcgg taattccgag ctgcaatacg tcagctaccg ccttggcgaa ccggtgtttg
     5101 acgtccagga atgtcaaatc cgtggcgtga cctattccgc accgctgcgc gttaaactgc
     5161 gtctggtgat ctatgagcgc gaagcgccgg aaggcaccgt aaaagacatt aaagaacaag
     5221 aagtctacat gggcgaaatt ccgctcatga cggacaacgg tacctttgtt atcaacggta
     5281 ctgagcgtgt tatcgtttcc cagctgcacc gtagtccggg cgtcttcttt gactccgaca
     5341 aaggtaaaac ccactcttcg ggtaaagtgc tgtataacgc gcgcatcatc ccttaccgtg
     5401 gttcctggct ggacttcgaa ttcgatccga aggacaacct gttcgtacgt atcgaccgtc
     5461 gccgtaaact gcctgcgacc atcattctgc gtgccctgaa ctacaccaca gagcagatcc
     5521 tcgacctgtt ctttgaaaaa gttatctttg aaatccgtga taacaagctg cagatggaac
     5581 tggtgccgga acgcctgcgt ggtgaaaccg catctttcga catcgaagct aacggtaaag
     5641 tgtacgtaga aaaaggccgc cgtattactg cgcgccacat tcgccagctg gaaaaagacg
     5701 acgtcaaact gatcgaagtc ccggttgagt acatcgcagg taaagtggtt gctaaagact
     5761 atattgatga gtctaccggc gagctgatct gcgcagcgaa catggagctg agcctggatc
     5821 tgctggctaa gctgagccag tctggtcaca agcgtatcga aacgctgttc accaacgatc
     5881 tggatcacgg cccatatatc tctgaaacct tacgtgtcga cccaactaac gaccgtctga
     5941 gcgcactggt agaaatctac cgtatgatgc gccctggcga gccgccgact cgtgaagcag
     6001 ctgaaagcct gtttgagaac ctgttcttct ccgaagaccg ctatgacctg tctgcggttg
     6061 gtcgtatgaa gttcaaccgt tctctgctgc gcgaagaaat cgaaggttcc ggtatcctga
     6121 gcaaagacga catcattgat gttatgaaaa agctcatcga tatccgtaac ggtaaaggcg
     6181 aagtcgatga tatcgaccac ctcggcaacc gtcgtatccg ttctgttggc gaaatggcgg
     6241 aaaaccagtt ccgcgttggc ctggtacgtg tagagcgtgc ggtgaaagag cgtctgtctc
     6301 tgggcgatct ggataccctg atgcctcagg atatgatcaa cgccaagccg atttccgcag
     6361 cagtgaaaga gttcttcggt tccagccagc tgtctcagtt tatggaccag aacaacccgc
     6421 tgtctgagat tacgcacaaa cgtcgtatct cggcactcgg cccaggcggt ctgacccgtg
     6481 aacgtgcagg cttcgaagtt cgagacgtac acccgactca ctacggtcgc gtatgtccaa
     6541 tcgaaacccc tgaaggtccg aacatcggtc tgatcaactc cctgtccgtg tacgcacaga
     6601 ctaacgaata cggcttcctt gagactccgt atcgtaaagt gaccgacggt gttgtaactg
     6661 acgaaattca ctatctgtct gctatcgaag aaggcaacta cgttatcgcc caggcgaact
     6721 ccaacctgga tgaagaaggc cacttcgtag aagacctggt aacttgccgt agcaaaggcg
     6781 aatccagctt gttcagccgc gaccaggttg actacatgga cgtatccacc cagcaggtgg
     6841 tatccgtcgg tgcgtccctg atcccgttcc tggaacacga tgacgccaac cgtgcattga
     6901 tgggtgcgaa catgcaacgt caggccgttc cgactctgcg tgctgataag ccgctggttg
     6961 gtacgggtat ggaacgtgct gttgccgttg actctggtgt aactgcggtt gctaaacgtg
     7021 gtggtgtcgt tcagtacgtg gatgcttccc gtatcgttat caaagttaac gaagacgaga
     7081 tgtatccggg tgaagcaggt atcgacatct acaacctgac caagtacacc cgttctaacc
     7141 agaacacctg tatcaaccag atgccgtgtg tgtctctggg tgaaccggta gaacgtggcg
     7201 acgtgctggc agacggtccg tccaccgacc tcggtgaact ggcgcttggt cagaacatgc
     7261 gcgtagcgtt catgccgtgg aatggttaca acttcgaaga ctccatcctc gtatccgagc
     7321 gtgttgttca ggaagaccgt ttcaccacca tccatattca ggaactggcg tgtgtgtccc
     7381 gtgataccaa gctggggccg gaagagatca ccgctgacat cccgaacgtg ggtgaagctg
     7441 cgctctccaa actggatgaa tccggtatcg tttacattgg tgcggaagtg accggtggcg
     7501 acattctggt tggtaaggta acgccgaaag gtgaaactca gctgacccca gaagaaaaac
     7561 tgctgcgtgc gatcttcggt gagaaagcgt ctgacgttaa agactcttct ctgcgcgtac
     7621 caaacggtgt atccggtacg gttatcgacg ttcaggtctt tactcgcgat ggcgtagaaa
     7681 aagataaacg tgcgctggaa atcgaagaaa tgcagctcaa acaggcgaag aaagacctgt
     7741 ctgaagaact gcagatcctc gaagcaggtc tgttcagccg tatccgtgct gtgctggtag
     7801 ccggtggcgt tgaagctgag aagctcgaca aactgccgcg cgatcgctgg ctggagctgg
     7861 gcctgaccga cgaagagaaa caaaatcagc tggaacagct ggctgagcag tatgacgaac
     7921 tgaaacacga gttcgagaag aaactcgaag cgaaacgccg caaaatcacc cagggcgacg
     7981 atctggcacc gggcgtgctg aagattgtta aggtatatct ggcggttaaa cgccgtatcc
     8041 agcctggtga caagatggca ggccgtcatg gtaacaaggg tgtaatttct aagatcaacc
     8101 cgatcgaaga tatgccttac gatgaaaacg gtactccggt agacatcgta ctgaacccgc
     8161 tgggcgtacc gtctcgtatg aacattggtc agatcctcga aacccacctg ggtatggctg
     8221 cgaaaggtat cggcgacaag atcaacgcca tgctgaaaca gcagcaagaa gtcgcgaaac
     8281 tgcgcgaatt catccagcgt gcgtacgatc tgggcgctga tgttcgtcag aaagttgacc
     8341 tgagtacctt cagcgatgaa gaagttatgc gtctggctga aaacctgcgc aaaggtatgc
     8401 caatcgcaac gccggtcttc gacggtgcga aagaagcaga aattaaagag ctgctgaaac
     8461 ttggcgacct gccgacttct ggtcagatcc gcctgtacga cggtcgcact ggtgaacagt
     8521 tcgaacgtcc ggtaaccgtt ggttacatgt acatgctgaa actgaaccac ctggtcgacg
     8581 acaagatgca cgcgcgttcc accggttctt acagcctggt tactcagcag ccgctgggtg
     8641 gtaaggcaca gttcggtggt cagcgtttcg gggagatgga agtgtgggcg ctggaagcat
     8701 acggcgcagc atacaccctg caggaaatgc tcaccgttaa gtctgatgac gtgaacggtc
     8761 gtactaagat gtataaaaac atcgtggacg gcaaccatca gatggagccg ggcatgccag
     8821 aatccttcaa cgtattgttg aaagagattc gttcgctggg tatcaacatc gaactggaag
     8881 acgagtaatt ctcgctcaaa caggtcactg ctgtcggggt aaaacccggc agcggattgt
     8941 gctaactccg acgggagcaa atccgtgaaa gatttattaa agtttctgaa agcgcagact
     9001 aaaaccgaag agtttgatgc gatcaaaatt gctctggctt cgccagacat gatccgttca
     9061 tggtctttcg gtgaagttaa aaagccggaa accatcaact accgtacgtt caaaccagaa
     9121 cgtgacggcc ttttctgcgc ccgtatcttt gggccggtaa aagattacga gtgcctgtgc
     9181 ggtaagtaca agcgcctgaa acaccgtggc gtcatctgtg agaagtgcgg cgttgaagtg
     9241 acccagacta aagtacgccg tgagcgtatg ggccacatcg aactggcttc cccgactgcg
     9301 cacatctggt tcctgaaatc gctgccgtcc cgtatcggcc tgctgctcga tatgccgctg
     9361 cgcgatatcg aacgcgtact gtacttcgaa tcctatgtgg ttatcgaagg tggtatgacc
     9421 aacctggagc gtcagcagat cctgactgaa gagcagtatc tggacgcgct ggaagagttc
     9481 ggtgacgaat tcgacgcgaa gatgggggcg gaagcaatcc aggccctgct gaagagcatg
     9541 gatctggagc aagagtgcga acagctgcgt gaagagctga acgaaaccaa ctccgaaacc
     9601 aagcgtaaaa agctgaccaa gcgtatcaaa ctgctggaag cgttcgttca gtctggtaac
     9661 aaaccagagt ggatgatcct gaccgttctg ccggtactgc cgccagatct gcgtccgctg
     9721 gttccgctgg atggtggtcg tttcgcgact tctgacctga acgatctgta tcgtcgcgtc
     9781 attaaccgta acaaccgtct gaaacgtctg ctggacctgg ctgcgccgga tatcatcgta
     9841 cgtaacgaaa aacgtatgct gcaggaagcg gtagacgccc tgctggataa cggtcgtcgc
     9901 ggtcgtgcaa tcaccggttc taacaagcgt cctctgaaat ctttggccga catgatcaaa
     9961 ggtaaacagg gtcgtttccg tcagaacctg ctcggtaagc gtgttgacta ctccggtcgt
    10021 tctgtaatca ccgtaggtcc atacctgcgt ctgcatcagt gcggtctgcc gaagaaaatg
    10081 gcgctggagc tgttcaaacc gttcatctac ggcaagctgg aactgcgtgg tcttgctacc
    10141 accatcaaag ctgcgaagaa aatggttgag cgcgaagaag ctgtcgtttg ggatatcctg
    10201 gacgaagtta tccgcgaaca cccggtactg ctgaaccgtg caccgactct gcaccgtctg
    10261 ggtatccagg catttgaacc ggtactgatc gaaggtaaag ctatccagct gcacccgctg
    10321 gtttgtgcgg catataacgc cgacttcgat ggtgaccaga tggccgttca cgtaccgctg
    10381 acgctggaag cccagctgga agcgcgtgcg ctgatgatgt ctaccaacaa catcctgtcc
    10441 ccggcgaacg gcgaaccaat catcgttccg tctcaggacg ttgtactggg tctgtactac
    10501 atgacccgcg actgtgttaa cgccaaaggc gaaggcatgg tgctgactgg cccgaaagaa
    10561 gcagaacgtc tgtatcgctc tggtctggct tctctgcatg cgcgcgttaa agtgcgtatc
    10621 accgagtatg aaaaagatgc taacggtgaa ttagtggcga aaaccagcct gaaagacacg
    10681 actgttggcc gtgccattct gtggatgatt gtaccgaaag gtctgcctta caccatcgtc
    10741 aaccaggcgc tgggtaaaaa agcaatctcc aaaatgctga acacctgcta ccgcattctc
    10801 ggtctgaaac cgaccgttat ttttgcggac cagatcatgt ataccggctt tgcatatgca
    10861 gcgcgttctg gtgcatctgt tggtatcgat gacatggtca tcccggagaa gaaacacgaa
    10921 atcatctccg aggcagaagc agaagttgct gaaattcagg agcagttcca gtctggtctg
    10981 gtaactgcgg gcgaacgcta caacaaagtt atcgatatct gggctgcggc gaacgatcgt
    11041 gtatccaaag cgatgatgga taacctgcaa actgaaaccg ttattaaccg tgacggtcag
    11101 gaagagaagc aggtttcctt caacagcatc tacatgatgg ccgactccgg tgcgcgtggt
    11161 tctgcggcac agattcgtca gcttgctggt atgcgtggtc tgatggcgaa gccggatggc
    11221 tccatcatcg aaacgccaat caccgcgaac ttccgtgaag gtctgaacgt actccagtac
    11281 ttcatctcca cccacggtgc tcgtaaaggt ctggcggata ccgcactgaa aacggcgaac
    11341 tccggttacc tgactcgtcg tctggttgac gtggcgcagg acctggtggt taccgaagac
    11401 gattgtggta cccatgaagg tatcatgatg actccggtta tcgagggtgg tgacgttaaa
    11461 gagccgctgc gcgatcgcgt attgggtcgt gtaactgctg aagacgttct gaagccgggt
    11521 actgctgata tcctcgttcc gcgcaacacg ctgctgcacg aacagtggtg tgacctgctg
    11581 gaagagaact ctgtcgacgc ggttaaagta cgttctgttg tatcttgtga caccgacttt
    11641 ggtgtatgtg cgcactgcta cggtcgtgac ctggcgcgtg gccacatcat caataaaggt
    11701 gaagcaatcg gtgttatcgc ggcacagtcc atcggtgaac cgggtacaca gctgaccatg
    11761 cgtacgttcc acatcggtgg tgcggcatct cgtgcggctg ctgaatccag catccaggtg
    11821 aaaaacaaag gtagcatcaa gctcagcaac gtgaagtcgg ttgtgaactc cagcggtaaa
    11881 ctggttatca cttcccgtaa caccgaactg aaactgatcg acgaattcgg tcgtaccaaa
    11941 gaaagctaca aagtacctta cggtgcggta ctggcgaaag gcgatggcga acaggttgct
    12001 ggcggcgaaa ccgttgcaaa ctgggacccg cacaccatgc cggttatcac cgaagtaagc
    12061 ggttttgtac gctttactga catgatcgac ggccagacca ttactcgtca gaccgacgaa
    12121 ttgaccggtc tgtcttcgct ggtggttctg gattccgcag aacgtaccgc aggtggtaaa
    12181 gatctgcgtc cggcactgaa aatcgttgat gctcagggta acgacgttct gatcccgggt
    12241 accgatatgc ctgcgcagta cttcctgccg ggtaaagcga ttgttcagct ggaagatggc
    12301 gtacagatca gctctggtga caccctggcg cgtattccgc aggaatccgg cggtaccaag
    12361 gacatcaccg gtggtctgcc gcgcgttgcg gacctgttcg aagcacgtcg tccgaaagag
    12421 ccggcaatcc tggctgaaat cagcggtatc gtttccttcg gtaaagaaac caaaggtaaa
    12481 cgtcgtctgg ttatcacccc ggtagacggt agcgatccgt acgaagagat gattccgaaa
    12541 tggcgtcagc tcaacgtgtt cgaaggtgaa cgtgtagaac gtggtgacgt aatttccgac
    12601 ggtccggaag cgccgcacga cattctgcgt ctgcgtggtg ttcatgctgt gactcgttac
    12661 atcgttaacg aagtacagga cgtataccgt ctgcagggcg ttaagattaa cgataaacac
    12721 atcgaagtta tcgttcgtca gatgctgcgt aaagctacca tcgttaacgc gggcagctcc
    12781 gacttcctgg aaggtgaaca ggttgaatac tctcgcgtca agatcgcaaa ccgcgaactg
    12841 gaagcgaacg gcaaagtggg tgcaacttac tcccgcgatc tgctgggtat taccaaagcg
    12901 tctctggcaa ccgagtcctt catctccgcg gcatcgttcc aggagaccac tcgcgtgctg
    12961 accgaagcag ccgttgcggg caaacgcgac gaactgcgcg gcctgaaaga gaacgttatc
    13021 gtgggtcgtc tgatcccggc aggtaccggt tacgcgtacc accaggatcg tatgcgtcgc
    13081 cgtgctgcgg gtgaagctcc ggctgcacca caggtgactg cagaagacgc atctgccagc
    13141 ctggcagaac tgttgaacgc aggtctgggc ggttctgata acgagtaatc gttaatccgc
    13201 aaataacgta aaaacccgct tcggcgggtt tttttatggg tgttattcag agggcaaagt
    13261 cttcacggga aatattaaga aaggcagggc gaaacgagga agaagctttt cgccctggac
    13321 aacattcctg ttgacggcac taccataaaa aaacacaacg ctttcaacaa ccttgcgttt
    13381 ttcatcttcc gtagcggctc ggaataaatt tacgttctta cattctgtct catggccttt
    13441 gcgatgggcg tcccagatag ctgtcccagt ctttccatac cggctgcaaa ccctgagcgg
    13501 ttaacgcggc agcaaccgct tccggtctgc gatcgtcgtg tggtgagaac tgttccagct
    13561 cggggtggtt gtcggcatag ccacctggct gcgttttcga aaaagcgctg acgttattaa
    13621 ttgccagcgg aattacgcga tcgcgaaacc acggtgattc ccgcgtggag agtgacagtt
    13681 caatctccgg tgcaagcagc cggaaagcgc agatggcttg cactaactgg cgttcatcca
    13741 taatcgacgc aggctcaatg ccgccagtac atggacgcag gcgtgggaag gagacagagt
    13801 agcggctttg ccagtaatgc tgttgcagcc atagcaaatg ttcggcaacc atatagcagt
    13861 caactcgcca gctgtcggaa aggccgatta gcgcgccgag gcctatctta tcaatccccg
    13921 cacgccctag ccgatccggc gtttccagcc gccagaagaa gtcctgtttt ttacctttca
    13981 gatgatgatg ggcataagtc gcctcatgat atgtctcctg ataaaccatt actccatcga
    14041 ggccaagctg ttttaactcg gcatattccg cctccgccag cggttgcact tccatctgta
    14101 gtgaagagaa ctgttcacgc agggcaggga gatgacgacg aaagtaatcc atccccactt
    14161 tcgcctgatg ttcaccagtg actaatagca gatgttcaaa gcccatctcc cgtatagcgg
    14221 cgctttccct ggcaatatcc gcttcatcca gcgttttgcg cttgatgcgg ttgctcatag
    14281 aaaatccgca ataagtacag tcgttagcgc aaagattgga aagataaagc gggacgtaga
    14341 agctgacgac gttgccaaat cgctgacgag tcagacgttg cgctcgctgg gccagtggtt
    14401 ccagatagcc actggcggcg ggtgatagca atgccatcat gtcgtcgcgg gtgagttgcg
    14461 aggcatttag cgcccgctct acgtcagcag ccgttttgcc gttgatacgc aggtggatgt
    14521 catcccagtc cagttgtcgc cagcgatcgc tgaaggtttt catgccgatg cctccagaaa
    14581 tccagtcagc gggctggtgg catgagcaaa atgactgcgg ctgcccggtc cggactgacg
    14641 tgccagcagg cctgcttcta ccgccagacg aaatgccttc gccatgttga cgggatcgtc
    14701 cgcgacggca atcgccgtat tcactaacac cgcgtcggcc cccatttcca gcgcctgcgc
    14761 ggcatggctg ggaacgccga tgccagcatc gacaaccacc ggaacggtgg cctgctggat
    14821 aataatctcc agcatggcgc gggtttccag tccctgattc gagccaatcg gcgcgccgag
    14881 cggcatcacc gctgcacagc cgacttcttc cagacgttta cacaataccg gatcggcccc
    14941 gcagtaaggc agcacgacaa atccctgttg taccagcgtt tcggcggctt tcagggtttc
    15001 gatgggatcg ggcaacagcc agcgggcgtc agggtgaatc tccaatttta gccagtttgt
    15061 gcctaacgct tcacgtgcca gatgggcggc gaaaatggct tcttccgcgg ttttcgcccc
    15121 ggaagtattc ggcagcaggg ccacacccgc cgcgataagc ggttcgagga tagcgtcgtt
    15181 gtgctggcgc aagtcgacac gtttcatcgc cagcgtcacc agttggctgc cggaagcgcg
    15241 aatagactcc accatcagtt gtgaagaggc gaattttccg gtgccagtaa acagatgtga
    15301 atcaaacgtt ttgtccgcaa tatgtaacat ctcaaccccc tgcaataact tgaaaaagca
    15361 ggatctggtc gccatcctgc acgatatgtt gcgcccattg ttcccgcggg acgatttgct
    15421 gattaatcgc cagtgccgca cctgcttgtt gttgttcgag ttgctccagc agttcgtgaa
    15481 cagttagttc ggcgacacac tgcatcggtt gatcgttaaa caggatctgc attgcatcct
    15541 ccacataccg ggcaaccgct ggcgcggcgc aacgtcagac tgcgccactg gctcgattta
    15601 ccgtcgaaca gtcggagttc tcccgcaggc gtctctatac cacttaataa cttaatcgct
    15661 tccagtgcct gtaaagtgcc cataaccccg accaccgggc caaccacgcc cgccgtgcgg
    15721 cagttgcgtt ctggctcctg gttatctggc cacaggcagc ggtaacatcc ctgctcccag
    15781 ggcggcgtca gtaccatcaa ctgaccgcca aatccgaccg cgctggcggt gataagcggc
    15841 gtgttgagtg ccacgcaggt ggcattaatc tcctggcgag tcgccatatt gtcagtacag
    15901 tcgagcacca catcggcttg tgcaaccgca tcttttaacg tctcacccgt taaccgttgt
    15961 tgtaatgcca tcagtcgaat atcgggattc aactgtgtca gtcgctgttg gctgacctgt
    16021 gatttcgggc gatcgatatc ttcagtggta aagaggattt gtcgttgcag attgcttaaa
    16081 tgcacatcgt cgtcatctgc cagtaccagc gtcccgacgc cagcccccgc caggtatagc
    16141 gcagcaggtg tacccagccc gccaagacca ataatcagca cctggctgtc gagcagtttt
    16201 tgctgcccgt ccagagcgat atcgtcgagc aggatttggc ggctataacg cataaagtca
    16261 cggtcattca tcgccaactc ctgcaatttc cagcagctgt gccgttgcca aacgccagtc
    16321 tgcggcttga gtaatggcgc tgacgacggc gatactgccg acacccgttg ctatcaccgc
    16381 aggcgcgcgc gccagactga taccgccaat cgccacggtg ggataatccg ccaggcgctc
    16441 aacatgccgt gccagctgtt ccagtccctg cggtgcagaa ggcatctgtt tggtttgtgt
    16501 cggaaacaca tgtcccagcg cgatataaga aggacgtgcc gccagcgcga cgtcgatctc
    16561 catatcgtca tgagtcgaaa cgcccagccg caggcccgct gcacggatgg cattgagatc
    16621 ggtggcttgc aaatcttcct gccccagatg gacgccatac gcctgatgct taatcgccag
    16681 ccgccagtaa tcgttgataa acaatcgcgc gttatagcgg cggcccagcg caattgccgc
    16741 caccacatcg gcttctacct cttcatcgcg ctgatctttg atgcgtagct ggagagtacg
    16801 tacgcctgca tccaacagac gttcgatcca ctgtacgctg tcaaccaccg ggtacagtcc
    16861 taaacgaaag ggtacaggag gaaactcagg ctgatacatc acgcttcctc cttacgcagg
    16921 tagatttctc cgcctctggc gcggaagttc tccgacatat ccgccattcc cacttcaatg
    16981 gtttgcgcgg cggcgtaatc acgcacttcc tggctgattt tcatcgagca gaatttcggt
    17041 ccacacatgg agcaaaaatg ggcgacttta ccggactctt gcggcagggt ttcatcgtga
    17101 taagcgcggg cagtaaacgg gtcgagggcc agattaaact ggtcttccca gcgaaattcg
    17161 aagcgggctt tcgacatggc gttatcgcga atttgcgcgc ccggatgccc tttcgccagg
    17221 tcggcggcgt gagcggcaat cttataggtg ataagcccct gtttaacatc ttctttattt
    17281 ggcaggccca gatgctcttt cggcgttacg tagcagagca tcgcgcagcc aaaccagcca
    17341 atcatcgccg cgccaatccc tgacgtgaag tggtcatagc ccggcgcgat atcggtagtt
    17401 agcggcccca gagtgtaaaa cggcgcttcg tggcagtgct ctaactcctc ggtcatattg
    17461 cggcgaatca tctgcatcgg cacgtggcct gggccttcaa tcatcacctg cacgtcatat
    17521 tcccaggcga ttttggtcag ttcgcccagc gtatgcagct cggcaaactg tgcttcatcg
    17581 ttggcgtcct gaatagaacc ggggcgcagg ccgtcgccca gcgacagcga aacgtcataa
    17641 gcagcacaga tttcacaaat ttcgcggaag tgttgataga ggaaattttc ctgatgatgg
    17701 gagaggcacc atttcgccat aatcgaaccg ccgcgagaga cgataccggt caggcgtttc
    17761 gcggtcatcg gcacgtagcg cagcagcacc cccgcatgga tagtgaagta atccacacct
    17821 tgctcggctt gttccagcag ggtgtcgcgg aagacttccc aggtaagatc ttcagcgatc
    17881 ccgttcacct tctccagtgc ctggtagatc ggcactgtac cgatcggcac cgggctgtta
    17941 cgcaaaatcc actcgcgggt ttcgtgaata tagcgaccag tggaaagatc catcacggta
    18001 tccgctcccc agcgcgtgga ccataccagt ttttccactt cttcttcgat ggaagaagtg
    18061 accgccgagt tgccgatatt ggcgttaact tttaccagga agttacggcc aataatcatc
    18121 ggctccgatt ccggatgatt aatgttggcc gggataatcg cacgtccggc agcaacttca
    18181 tcacggacaa attccgcagt gatattttcc ggcagacgtg cgccaaagct cattcccgga
    18241 tgctggtggc gtaaaacttc gctacgtatg cgctcgcgac ccatattctc gcgaatggcg
    18301 atgaattcca tttctggcgt gatgataccc ttgcgggcgt agtgcagttg ggtgacgcgg
    18361 cgtcctgctt tggcgcgttt tggcgttaat acgccgctaa aacgcagctc gtcaaggcca
    18421 tcatctgcca gccgcgcttt agtgtaatcg gaactgcgta cggtaagttc ttcggtatcg
    18481 ccgcgcgcat cgatccacgg ctggcgtagt tttgccagcc cttgctgcac gttaatggca
    18541 atctgcggat cgccatacgg gccggaggtg tcgtagaccg gaatcgcttc gttttcttcg
    18601 tactgcggct gttctttgct gccgccaatt agcgtcgggc taagctggat ctcacgcatc
    18661 ggcacgcgca cgccggggtg tgtgccagtg atgtaaatgc gttttgagtt gggaaaggcg
    18721 gagccttcca gggtgtcgat aaaatgttgg gcctgggcgc gctgttcgcg gcgggtcagt
    18781 tttgttgcag acatagctca ttccaaaaag ttaaggacgt ggcttgtcag acgacggatg
    18841 aagcaagaga cgatcgccgc aaaggcgatg cgatagcaga ttaactcttg ttcccttcgc
    18901 aggtattagc ctgatcaggt tccgcggatc ccgaataaac ggtctcagcc agttaaggca
    18961 ctccgacaag aaattagccc cacaaacggg gcgttgaatg taaattacgc gttaacagcg
    19021 cagaactcaa gcaggatatt tgacgcgggc ggcgtcaagc accagtagaa tcagcttatc
    19081 ttccagcaca aagcgcgctt ccagtgcctc gccgatgtca gataaaacct gttgaaactc
    19141 aaggtaatta tcatgatcga tggcggtttc caggctggaa tcgtagtcat ccataatctg
    19201 ttgagtattg gcttcgagtt gcggccaaat cttcgcggcg cgcgcgagtt gcccgttgcc
    19261 ttccagctta tgaagaatgc gctcataaat actgaaatgt ccggcagaca agtaatcgac
    19321 caggctctga caaaaatcat caagggcttt ttcgtttagc ctcatgtacg attctttgcc
    19381 aggcttaatg ccaaccagat tgtagtaagc cacgagcaga tgcttacgta catgtagcca
    19441 gcgatcaacc agtttgttac ttcctctgac gcgctccgtc aggttatcga gctggttaag
    19501 catgattgac tccgcaagtt tgtattcaaa aactgctctg tgagaaatgt aaaaaccatg
    19561 ttaaacatgc cagtgatgca aaggtagtgc aagagctatg gatcgtataa ttgaaaaatt
    19621 agatcacggc tggtgggtcg tcagccatga acaaaaatta tggttgccga agggagaatt
    19681 gccatatggc gaagcggcaa atttcgatct tgtgggtcag cgcgcactac aaatcggcga
    19741 atggcagggg gaacctgttt ggttagtaca acagcagcgg cgtcacgata tggggtcggt
    19801 acgtcaggtc attgatctcg atgttgggct gtttcaactg gccggacgtg gcgtgcaact
    19861 ggcggagttt taccgatcgc ataaatactg tggttactgc gggcatgaaa tgtatccaag
    19921 caaaaccgaa tgggcgatgc tgtgcagcca ttgccgtgag cgttactacc cgcaaatcgc
    19981 cccctgcatt attgttgcca tccgtcgcga tgattcgatc ctcctcgccc agcatacccg
    20041 ccatcgtaac ggtgtccata cagtacttgc cggattcgtc gaagtgggtg aaaccctcga
    20101 gcaggcagtc gcgcgggaag tgatggaaga gagcggaatt aaagttaaaa acttgcgtta
    20161 cgtgacctcc caaccgtggc cgtttcctca gtcgttaatg accgcgttta tggcggaata
    20221 tgacagcggc gagatcgtga tcgacccgaa agaattgctt gaggcgaact ggtatcgcta
    20281 tgacgatttg ccgttactcc cgccgcccgg caccgtagcg cgccgtctga tagaagatac
    20341 ggtggcgatg tgtcgggcag agtatgagtg atgatacact gaccgcctga cgcactaagg
    20401 aacagccaaa atgaccgaac ttaaaaacga tcgttatctg cgggcgctgc tgcgccagcc
    20461 cgttgatgtc actccagtat ggatgatgcg ccaggcgggt cgctatctac cggaatataa
    20521 agccacgcgc gcccaggcgg gcgattttat gtcgctgtgc aaaaatgccg agctggcgtg
    20581 cgaagtgact ttgcaaccgc tgcgtcgcta tccgctggat gcggcgatcc tcttttccga
    20641 tatcctcacc gtgccagacg cgatgggttt agggctctat tttgaagccg gagaaggtcc
    20701 gcgttttacc tcgccagtca cctgcaaagc cgatgtcgat aaactgccaa ttccggaccc
    20761 ggaagatgag ctgggttacg tgatgaacgc ggtgcgtacc attcgtcgcg aactgaaagg
    20821 cgaagtgccg ctgattggtt tttccggcag cccgtggacg ctggcgacct acatggtgga
    20881 aggcggcagc agcaaagcgt tcaccgtgat caaaaaaatg atgtatgccg atccacaggc
    20941 gctgcacgcg ctactcgata aactggcgaa aagcgtcact ttgtatctga atgcgcagat
    21001 taaagccggt gctcaggcag tgatgatttt cgacacctgg ggcggcgtgc ttaccgggcg
    21061 cgattatcaa cagttctcgc tctattacat gcataaaatt gttgatggtt tactgcgtga
    21121 aaacgacggt cgccgcgtac cggtcacgct gtttaccaaa ggtggcggac agtggctgga
    21181 agcgatggca gaaaccggtt gcgatgcgct gggcctcgac tggacaacgg acatcgccga
    21241 tgcgcgccgt cgcgtgggca ataaagtcgc gctgcagggt aatatggacc cgtcgatgct
    21301 gtatgcgccg cctgcccgca ttgaagaaga agtggcgtct atacttgccg gtttcggtca
    21361 cggcgaaggc catgtcttta accttggtca cggcattcat caggatgtgc cgccagaaca
    21421 tgctggcgtg tttgtggagg cagtgcatcg cttgtctgaa cagtatcacc gctaaggagc
    21481 gattatggat ctcgcgtcat tacgcgctca acaaattgaa ctggcttctt ctgtgatccg
    21541 cgaggatcga ctcgataaag atccgccgga tctgatcgcc ggagccgatg tcggatttga
    21601 gcagggcgga gaagtgacgc gagcggcgat ggtgctgctg aaatacccct cgcttgagct
    21661 ggtggagtat aaagtggccc gcatcgccac caccatgcct tacattccag gttttctctc
    21721 gttccgcgaa tatcctgcgc tgctggcagc gtgggagatg ctgtcgcaaa agccggattt
    21781 agtgtttgtc gatggtcatg ggatctcgca tcctcgccgt cttggcgtag ccagccattt
    21841 tggcttattg gtggatgtgc cgaccattgg cgtggcgaaa aaacggctct gcggtaaatt
    21901 cgaaccgctc tccagcgaac cgggcgcgct ggcccctctg atggataaag gcgagcagct
    21961 ggcctgggtc tggcgcagca aagcgcgctg taacccgttg tttatcgcta ccggccatcg
    22021 ggtcagcgtg gacagcgcgc tggagtgggt acaacgctgc atgaaaggct atcgtctgcc
    22081 ggagccaacg cgctgggcgg acgcggtggc ctcggaacgt ccggcgttcg tgcgctatac
    22141 agcaaatcag ccctaattca ggtaaactgc ggataatttc cgtatttgag aactcaacat
    22201 gttacaaaac ccaattcatc tgcgtctgga gcgcctggca agctggcagc atgtcacttt
    22261 tatggcctgc ttatgcgaac gcatgtaccc caattacgcc gtattctgtc agcaaaccgg
    22321 cttcggcgat gggcagattt accgccgcat tctcgatctc atttgggaaa cgctgaccgt
    22381 caaagatgca aaagtaaatt tcgacagcca actggagaaa tttgaagaag cgattccttc
    22441 agccgacgat ttcgatctgt acggcgttta tccggcaatc gatgcttgcg tggcgttaag
    22501 tgaactggtt cattcgcgtt taagtggcga aacgctcgaa cacgcggtgg aagtgagtaa
    22561 gacctccatc acgaccgttg cgatgctgga aatgactcag gctggtcgcg aaatgagcga
    22621 tgaagagctc aaagaaaacc cagctgtaga gcaagaatgg gacattcagt gggaaatatt
    22681 ccgactttta gctgagtgtg aagaacgcga tatcgagctg ataaaaggcc ttagggcaga
    22741 cctgcgtgag gcgggtgaga gcaatattgg tataattttt cagcaataag accagaaaac
    22801 gtgatttaac gcctgatttg tcgtacctgg agtcttccct ttcgcccccc gtctggtcta
    22861 catttggggg gcgaaaaaaa gtggctatcg gtgcgtgtat gcaggagagt gctattctgg
    22921 catttccgtc gcactcgatg cttagcaagc gataaacaca ttgtaaggat aacttatgaa
    22981 caagactcaa ctgattgatg taattgcaga gaaagcagaa ctgtccaaaa cccaggctaa
    23041 agctgctctg gagtccactc tggctgcaat tactgagtct ctgaaagaag gcgatgctgt
    23101 acaactggtt ggtttcggta ccttcaaagt gaaccaccgc gctgagcgta ctggccgcaa
    23161 cccgcagacc ggtaaagaaa tcaaaattgc cgcagctaac gtaccggcat ttgtttctgg
    23221 caaggcactg aaagacgcag ttaagtaaga ttgcgtggca gtgaacagtt ttaacgaagg
    23281 ggtggtttca ccccttttgt ctttctggcg tcgatcattg atgctggcag gtgcgctgtt
    23341 tctcactgcc tgtagtcaca actcttcact tcctcccttt accgccagtg gatttgctga
    23401 agaccagggc gcggtacgca tctggcgaaa agacagcggc gataatgtgc atcttcttgc
    23461 cgtgtttagc ccgtggcgca gtggcgatac gacgacgcga gagtatcgct ggcagggcga
    23521 taacctcacg cttatcaata tcaacgttta cagcaaaccg ccggtaaaca tccgggcgcg
    23581 ttttgacgat cgcggtgact tgagctttat gcaacgtgaa tccgacgggc aaaagcagca
    23641 gctttccaat gatcagatcg atttataccg ttatcgtgcc gcgcagatcc gcgagattag
    23701 cgatgcctta cgccagggga gagtcgtgct gcgccagggg cgctggcatg cgatggagca
    23761 gactgtgacc acctgcgaag ggcaaaccat taaacctgat ttagattcgc aggcgatagc
    23821 gcatatcgag cgccgccaga gccgttcttc tgttgatgtc agcgtggcat ggctggaagc
    23881 gcccgaaggt tcgcaattac tgttggtggc aaactctgat ttctgtcgct ggcaacccaa
    23941 cgagaaaacg ttctgattta ccagtggccc atacccatat gaccgccacc accgcagccg
    24001 ccgtaaccca ttccggtacc gcgcggaata cccgcttcag ccatcgcaat atctcgtttc
    24061 acccgtaact catctaacga ctgacgcaaa ttctccatct ctttggcgac cgcgttaatt
    24121 ttgctgctat ccggtgggtt cgcggctaac agggcattgt attcataacg ctttgtcacc
    24181 agttgctgtt gcagtgcgct gctttgagcg taaaagtcat tatggatttt ctgccacgct
    24241 gtttgttgtt cgctggtcaa aggcgcggca ttttgctgcc acataccgtg tccgccgtga
    24301 gcaaatgccg atgtcgatcc catcgccatt gctgaaagcg ccatcattac cagggcaatt
    24361 ttcgtgttcc gtttcatggt taatcctcca gtggttgtct tacttcgggt attgcatctt
    24421 tcgtgccaac ggcgaaacgc tgatatgacg ggtaatctgg catgataaac gagtaaaaat
    24481 gactcgcctg ttgtgggtag cgagtcattt ttactcattg aaacttaagc ctttgtgtta
    24541 cagcgcaggg taagcgctga taaaagatgg catgatttct gctgtcagaa agggatgggc
    24601 aggcaaagaa gaagatgcgt tttatgcaac gttctaaaga ctccttagct aaatggttaa
    24661 gcgcgatcct ccccgtggtc attgttgggc tggtggggct gtttgcggtg acagtgattc
    24721 gcgattatgg gcgcgagact gccgccgcca gacaaacgct gctggaaaaa ggcagtgtac
    24781 ttatccgtgc tctcgaatcc ggctcgcgcg tcggaatggg gatgcgcatg catcatgcgc
    24841 agcagcaggc cttactggaa gaaatggccg ggcagcctgg agtacgttgg tttgcggtca
    24901 cggatgaaca aggaacaatc gtgatgcata gcaactccgg catggtggga aaacagcttt
    24961 attccccgca ggaaatgcag cagttacatc cgggagatga agaagcgtgg cggcggatcg
    25021 atagcgcaga cggcgagcct gttctggaaa tttatcgcca gtttcaaccg atgtttgctg
    25081 ctggaatgca ccggatgcgc catatgcagc agtatgccgc gacaccacaa gcaattttta
    25141 tcgctttcga cgccagtaac attgtgagtg ccgaagatcg tgagcagaga aacaccctga
    25201 ttatcctctt cgccctggcg acggtcttgc tggcaagcgt gttgtcattc ttctggtatc
    25261 gccgatatct gcgctcgggc cagctgttgc aggatgaaat gaagcgcaaa gagaagctgg
    25321 tggcactggg gcatcttgcg gcaggcgttg cccacgaaat ccgtaaccca ctttcctcga
    25381 ttaaagggct ggcgaaatac tttgccgaac gcgcgccagc agggggagaa gcgcatcaat
    25441 tggcgcaggt gatggcgaaa gaagccgacc gtttaaaccg cgtggtaagc gagctgctgg
    25501 aactggttaa gccaacgcat ctggctttgc aggcggtgga gctcaacacg ctgattagcc
    25561 actcattaca gctggtaagc caggatgcaa acagccggga gatccagtta cgctttaccg
    25621 ccaacgacac attaccggaa attcaggccg atccggacag gctgactcag gtcctgttga
    25681 atctctatct caacgctatt caggcgattg gtctgcatgg cgtgattagc gtgacggcca
    25741 gcgaaagcgg cgcgggcgtg aaaatcagcg ttaccgacag cggtaaggga attgcggcgg
    25801 atcagcttga agccatcttc actccgtact tcaccaccaa agccgaaggc accggactgg
    25861 ggctggcggt cgtgcataat attgttgaac aacacggtgg tacaattcag gttgcaagcc
    25921 aggaggggaa aggcgcaacg ttcaccctct ggcttccggt caatattacg cgtaaggacc
    25981 cacaaggatg acgcacgata atatcgatat tctggtggtg gatgatgaca ttagccactg
    26041 cactattttg caggctttac tgcgcggctg gggctataac gtcgcgctgg cgaacagcgg
    26101 gcgacaggcg ctggagcagg tgcgggaaca ggttttcgat cttgtgcttt gcgatgtgcg
    26161 aatggcagag atggacggca tcgccacgct aaaagagatc aaagcgttaa atccggcgat
    26221 tccggtactg attatgaccg cgtactccag cgtcgagacg gcggtagagg cactgaaaac
    26281 cggggcgctg gattacctca tcaagccgct ggatttcgat aacctgcagg caacgctgga
    26341 aaaggcgctc gcgcatacgc acagtatcga cgctgaaacg cctgcggtgt ctgccagcca
    26401 gttcggtatg gtcggtaaaa gcccggcgat gcaacacctg ctcagtgaaa tcgccctcgt
    26461 cgcgccatcg gaagccacgg tgctgatcca cggcgattcc ggtaccggta aagagctggt
    26521 cgccagggcg attcacgcca gtagcgcacg tagcgaaaaa ccgctggtaa cgctcaactg
    26581 tgcggcactc aacgaatcct tgctggaatc tgaattgttc ggtcatgaaa aaggggcgtt
    26641 taccggagcc gacaaacggc gagaagggcg ctttgttgag gcggacggcg gcacgctgtt
    26701 tctcgatgag attggcgata tctcgccgat gatgcaggtt cgtctgctgc gtgcgattca
    26761 ggagcgcgaa gtgcaacgtg tcggcagcaa ccagactatc tcggttgatg tccggctgat
    26821 tgcggcgacc catcgcgatc ttgccgcaga ggtgaatgcc gggcgttttc gccaggatct
    26881 ctactatcgg ctgaacgtgg tggcgattga agtaccatcg ctgcggcaac ggcgggaaga
    26941 tattcctctg ctggctggcc attttcttca gcgctttgcc gaacgtaatc gcaaggcggt
    27001 aaaaggcttt acgccccagg cgatggatct gctgattcat tacgactggc cgggaaatat
    27061 tcgtgaactg gaaaacgcgg tggaacgggc ggtggtgctg ctgaccgggg aatatatttc
    27121 cgaacgcgag ctgccgctgg cgattgccag tacgccgatc ccgctggtac aaagtcagga
    27181 tattcagccg ctggtggaag tcgaaaaaga ggtgattctg gcggcgctgg agaaaacggg
    27241 cggcaacaaa accgaagccg cccgccagtt agggatcacg cgcaaaacgc tattggcaaa
    27301 actgtcgcgt tagttctgct cgcgttcgat agcgcgccag ccgatatctt tccggcagaa
    27361 gcagtcgtcc caatggatat cggtcattaa ggcataagcg cgtttctgcg cttctgccac
    27421 ggtatgaccc agcgcggtaa cgcacagtac gcgcccgccg ctggtcacta cctgctcgtc
    27481 atccgccagt ttcgtgcccg cgtggaacac tttgccgtct gccacttctt ccagcggcag
    27541 gccgtggatc acatcgccgg tgcggtaatc gcccggatat ccacccgcag ccatcaccac
    27601 gccgagagaa gcgcgttcat cccactcgga cgttttctcg tccagcttgc cttcacaggc
    27661 cgccaggcag agctcaacca gatcggactt catgcgcagc ataatcggct gggtttccgg
    27721 atcgccaaag cggcagttaa attcaataac ctttggatta ccctgtttgt cgatcatcag
    27781 gcccgcatag agaaaaccgg tgtaggtgtt gccttccgcc gccatgcctt tcacggttgg
    27841 ccagatgata cgttccatgg tgcgctgatg aacttcatcg gtcactaccg gcgcaggaga
    27901 gtaagccccc attccgccgg tgtttggccc ggtatcttta tcgcctacgc gtttgtgatc
    27961 ctggctggtg gccatcggta gcacatgctc gccgtccacc atcacgataa agctcgcttc
    28021 ttcaccgtcg aggaactctt cgataacaat acgatgaccc gcgtcgccaa acgcgttgcc
    28081 cgccagcata tcgtgaacag ctgcttcagc ttcttccagc gtcatcgcca cgataacgcc
    28141 tttcccggct gccagaccgt cagctttaat gacgattggc gcgcctttct cacgcagata
    28201 tgccagcgca ggttctacct cggtgaagtt ctggtattcc gctgtgggaa tgttatggcg
    28261 ggccagaaag tctttggtga atgctttaga accttccagt tgtgcagcac ctgcggttgg
    28321 gccgaagatt ttcagcccgg cggcgcggaa ggtatcgact acgcctttca ccagcggcgc
    28381 ttccgggccg acgatggtta gatctacctt ttcgttttgt gcgaaatcca gcagcgccgg
    28441 gatatcggtt acgccgatag cgacgttctg tagcgcgggt tccagcgcag tgcctgcatt
    28501 gcccggagca acaaaaacag tttcaaccag cggcgactga gccgctttcc aggccagcgc
    28561 gtgctcgcgc ccgccgttac cgatcactaa tactttcatc tgtcgctcca ttaatggcgg
    28621 aagtggcgca tgtcggtgaa gagcatcgca ataccgtgct cgtcggcggc ggcaattact
    28681 tcgtcatcac ggatagaacc gccaggctgg attacgcagg tcacgcccgc agcggctgcg
    28741 gcatcaatac cgtcgcggaa cgggaagaac gcgtcagaag ccattgacga acctttcact
    28801 tccaaacctt catcggcagc tttaataccg gcgattttcg cggagtacac gcggctcatc
    28861 tggcctgcgc caataccgat ggtcatattg tttttggcat agacgatagc gttggatttc
    28921 acgaacttcg ccactttcca gcagaacagc gcatcacgaa gttcctgttc agtcggctga
    28981 cgtttggtga cgacgcgcag ttcttccgca ccgaccatcc ccagatcgcg atcctgaacc
    29041 agtagaccgc cgttcacgcg tttgaaatca agacccggaa tacgttcgcc ccactgaccg
    29101 caggtcagaa cgcgtacgtt ctgcttggcg gcggtgattt tcagggcttc ttcgctggcg
    29161 gacggcgcaa taatcacttc gacgaactga cgagaaatga tggcctgcgc ggtttcagca
    29221 tccagttcgc ggttaaaggc aatgatgccg ccgaatgcgg aggttgggtc ggttttgtac
    29281 gcgcgatcgt aagcatcaag aatggaattg ccgatagcca cgccgcaagg gttggcgtgc
    29341 ttcacaatca cacatgccgg ctcggcgaac tctttcacgc actccagcgc cgcatcggta
    29401 tcggcgatgt tgttataaga gagagcttta ccctgaacct gggttgcggt agcaacggag
    29461 gcttctttca cattctcttc tatatagaag gcagcctgct ggtggctgtt ctcgccgtaa
    29521 cgcatatcct gcttcttaat gaagttcagg ttcagcgtgc gtgggaagcg accggcggct
    29581 tctttgcttt caccgtggta agccggaacc atgctgccga agtagttggc aatcatgctg
    29641 tcgtaggcgg cagtgtgttc gaaggctttg atggcgaggt cgaaacgggt tgcaagcgtc
    29701 agcgatcctt cgttggcatc catctcttta ataatggcgt cgtagtcgct gctcttcacc
    29761 acgattgcga catctttatg gttcttggcg gcggagcgca ccatcgttgg gccgccgata
    29821 tcgatgttct ctaccgcatc ttccagcgag cagccttcac gagcgacggt ctgggcgaac
    29881 ggatacaggt taacaaccac catatcgata ggctggatct gatgttcttc cataatggca
    29941 tcgtcctggc cgcgacggcc cagaatgcca ccatgtactt tcggatgcag ggtcttcacg
    30001 cgtccatcca tcatctccgg gaaaccggtg taatcggaaa cttcggttac cggcagacat
    30061 ttttctgcta acagacgggc agtgccccct gtagacagca gctccacacc gcgtgcggaa
    30121 agtgcctggg cgaattcgac gataccggct ttgtcagaaa cactgagcag agcgcggcgg
    30181 actggacgac gttgttgcat ggtaaatccc ctggatttga ctattacaga gagcgttagc
    30241 tgaatttttc gcgaaaaact cagctaacgc ccctaacggg gcatccttat ttttcgcccg
    30301 cattgtaacg aaaacgtttg cgcaacgctc gcgaattttt ctctttcaat ggtgatcaca
    30361 attttgacag tggctaccgt gggaaaaata cagaaattac attgatgatt gtggataact
    30421 ctgtgtgtaa aaaggtataa agcggggttt tgctggggaa tgcagcagtc agtcattttt
    30481 ctgcaatttt tctattgcgg cctgcggag
//
LOCUS       DAHHTG010000053        30253 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.053, whole genome shotgun sequence.
ACCESSION   DAHHTG010000053 DAHHTG010000000
VERSION     DAHHTG010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 30253)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30253)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30253
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.053"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            210..1793
                     /locus_tag="NBI74_002283"
     CDS             210..1793
                     /locus_tag="NBI74_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HCD1932607.1"
                     /translation="MEWIADPSIWAGLITLIVIELVLGIDNLVFIAILAEKLPPKQRD
                     RARVTGLLLAMLMRLLLLASISWLVTLTQPLFSFRSFTFSARDLIMLFGGFFLLFKAT
                     MELNERLEGKDSNNPTQRKGAKFWGVVTQIVVLDAIFSLDSVITAVGMVDHLLVMMAA
                     VVIAISLMLMASKPLTQFVNSHPTIVILCLSFLLMIGFSLVAEGFGFVIPKGYLYAAI
                     GFSVMIEALNQLAIFNRRRFLSANQTLRQRTTEAVMRLLSGQKEDAELDAETASMLVD
                     HGNQQIFNPQERRMIERVLNLNQRTVSSIMTSRHDIEHIDLNAPEDEIRQLLERNQHT
                     RLVVTDGDDAEDLLGVVHVIDLLQQSLRGEPLNLRVLIRQPLVFPETLPLLPALEQFR
                     NARTHFAFVVDEFGSVEGIVTLSDVTETIAGNLPNEVEEIDARHDIQKNADGSWTANG
                     HMPLEDLVQYVPLPLDEKREYHTIAGLLMEYLQRIPKPGEEVQVGNYLLKTLQVESHR
                     VQKVQIIPLRKDGEMEYEV"
     gene            complement(2245..4098)
                     /gene="asmA"
                     /locus_tag="NBI74_002284"
     CDS             complement(2245..4098)
                     /gene="asmA"
                     /locus_tag="NBI74_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane assembly protein AsmA"
                     /protein_id="HCD1932608.1"
                     /translation="MRRFLTTLMILLVVLVAGLSALVLLVNPNDFRDYMVKQVAARSG
                     YQLQLDGPLRWHVWPQLSILSGRMSLTAQGASQPLVRADNMRLDVALLPLLSHQLSVK
                     QVMLKGAVIQLTPQTEAVRSEDAPVAPRDNTLPDLSDDRGWSFDISSLKVADSVLVFQ
                     HEDDEQVTIRNIRLQMEQDPQHRGSFEFSGRVNRDQRDLTISLNGTVDASDYPHDLTA
                     AIEQINWQLQGADLPKQGIQGQGSFQVQWQESHKRLSFNQISLTANDSTLSGQAQVTL
                     TEKPEWQLRLQFPQLNLDNLIPLNETANGENGAAQQGQSQSTLPRPVISSRIDEPAYQ
                     GLQGFTADILLQASNVRWRGMNFTDVATQMTNKSGLLEITQLQGKLNGGQVSLPGTLD
                     ATSINPRINFQPRLENVEIGTILKAFNYPISLTGKMSLAGDFSGADIDADAFRHNWQG
                     QAHVEMTDTRMEGMNFQQMIQQAVERNGGDVKAAENFDNVTRLDRFTTDLTLKDGVVT
                     LNDMQGQSPVLALTGEGMLNLADQTCDTQFDIRVVGGWNGESKLIDFLKETPVPLRVY
                     GNWQQLNYSLQVDQLLRKHLQDEAKRRLNDWAERNKDSRNGKDVKKLLEKM"
     gene            complement(4120..4701)
                     /gene="dcd"
                     /locus_tag="NBI74_002285"
     CDS             complement(4120..4701)
                     /gene="dcd"
                     /locus_tag="NBI74_002285"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004942378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HCD1932609.1"
                     /translation="MRLCDRDIEAWLDEGRLSINPRPPVERINGATVDVRLGNKFRTF
                     RGHTAAFIDLSGPKDEVSAALDRVMSDEIVLDEGEAFYLHPGELALAVTLESVTLPAD
                     LVGWLDGRSSLARLGLMVHVTAHRIDPGWSGCIVLEFYNSGKLPLALRPGMLIGALSF
                     EPLSGPAARPYNRREDAKYRNQQGAVASRIDKD"
     gene            complement(4793..5434)
                     /gene="udk"
                     /locus_tag="NBI74_002286"
     CDS             complement(4793..5434)
                     /gene="udk"
                     /locus_tag="NBI74_002286"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HCD1932610.1"
                     /translation="MTDQSHQCVIIGIAGASASGKSLIASTLYRELREQVGDEHIGVI
                     PEDCYYKDQSHLSMEERVKTNYDHPSAMDHSLLLEHLQALKRGSAIDLPVYSYVEHTR
                     MKETVTVEPKKVIILEGILLLTDARLRDELNFSIFVDTPLDICLMRRIKRDVNERGRS
                     MDSVMAQYQKTVRPMFLQFIEPSKQYADIIVPRGGKNRIAIDILKAKISQFFE"
     gene            5752..9069
                     /locus_tag="NBI74_002287"
     CDS             5752..9069
                     /locus_tag="NBI74_002287"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HCD1932611.1"
                     /translation="MSKQSQHVLIALPHPLLHLVSLGLVSFIFTLFSLELSQFGTQLA
                     PLWFPTSIMMVAFYRHAGRMWPGIALSCSLGNIAASILLFSTSSLNMTWTTINIVEAV
                     VGAVLLRKLLPWYNPLQNLADWLRLALGSAIIPPLLGGVLVVLLTPGDDPLRAFLIWV
                     LSESIGALALVPLGLLFKPHYLLRHRNPRLLFESLLTLAITLTLSWLSMLYLPWPFTF
                     IIVLLMWSAVRLPRMEAFLIFLTTVMMVSLMMAADPSLLATPRTYLMSHMPWLPFLLI
                     LLPANIMTMVMYAFRAERKHISESETRFRNAMEYSAIGMALVGTEGQWLQSNKALCQF
                     LGYSQEELRGLTFQQLTWPEDLNKDLQQVEKLISGEINTYSMEKRYYNRNGDVVWALL
                     AVSLVRHTDGTPLYFIAQIEDINELKRTEQVNQQLMERITLANEAGGIGIWEWELKPN
                     IFSWDKRMFELYEIPPHIKPNWQVWYECVLPEDRQHAEKVIRDSLQSRSPFKLEFRIT
                     VKDGIRHIRALANRVLNKEGEVERLLGINMDMTEVKQLNEALFQEKERLHITLDSIGE
                     AVVCIDMAMKITFMNPVAEKMSGWTQEEALGVPLLTVLHITFGDNGPLMENIYSADTS
                     RSAIEQDVVLHCRSGGSYDVHYSITPLSTLDGSNIGSVLVIQDVTESRKMLRQLSYSA
                     SHDALTHLANRASFEKQLRILLQTVNSTHQRHALVFIDLDRFKAVNDSAGHAAGDALL
                     RELASLMLSMLRSSDVLARLGGDEFGLLLPDCNVESARFIATRIISAVNDYHFIWEGR
                     VHRVGASAGITLIDDNNHQAAEVMSQADIACYASKNGGRGRVTIYEPQQVAAHSERAV
                     MSLDEQWRMIKENQLMMIAHGVASPRIPQARNLWLISLKLWSCEGEIIDEQTFRRSFS
                     DPALSHALDRRVFHDFFQQAAKAIASKGLSIALPLSVAGLSSATLVNELIELLENSPL
                     PPRLLHLIIPADAILDHAESVQKLRLAGCRIVFSQVGRDLQIFNSLKANMADYLLLDG
                     ELCANVQGNLMDEMLITIIQGHAQRLGMKTIAGPVVLPLVMDTLSGIGVDLIYGDVIA
                     DAQPLDLLVNSSYFAIN"
     gene            complement(9179..10027)
                     /gene="alkA"
                     /locus_tag="NBI74_002288"
     CDS             complement(9179..10027)
                     /gene="alkA"
                     /locus_tag="NBI74_002288"
                     /EC_number="3.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase 2"
                     /protein_id="HCD1932612.1"
                     /translation="MYTLNWQPPYDWSWMLGFLAARAVNGVETVADSYYARSLAVGEY
                     RGVVTAIPDIARHTLHINLSAGLEPVAAECLAKMSRLFDLQCNPQIVNGALGKLGAGR
                     PGLRLPGSVDAFEQGVRAILGQLVSVAMAAKLTARVAQLYGERLDDFPDYVCFPTPHR
                     LAAADPQALKALGMPLKRAEALIHLANAALEGTLPMTIPGDVEQAMKTLQTFPGIGRW
                     TANYFALRGWQAKDVFLPDDYLIKQRFPGMTPAQIRRYAERWKPWRSYALLHIWYTEG
                     WQPDEA"
     gene            10161..11513
                     /gene="yegD"
                     /locus_tag="NBI74_002289"
     CDS             10161..11513
                     /gene="yegD"
                     /locus_tag="NBI74_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HCD1932613.1"
                     /translation="MFIGFDYGTANCSVAVMRDGKPHLLKMENDSTLLPSMLCAPTRE
                     AVSEWLYRHHDVPADDDETQALLRRAIRYNREEDIDVTAKSVQFGLSSLAQYIDDPEE
                     VWFVKSPKSFLGASGLKPQQVALFEDLVCAMMLHIRQQAQAQLPEAITQAVIGRPINF
                     QGLGGDEANTQAQGILERAAKRAGFKDVVFQYEPVAAGLDYEATLQEEKRVLVVDIGG
                     GTTDCSLLLMGPQWRSRLDREASLLGHSGCRIGGNDLDIALAFKNLMPLLGMGGETEK
                     GIALPILPWWNAVAINDVPAQSDFYSSANGRLLNDLVRDAREPEKVALLQKVWRQRLS
                     YRLVRSAEESKIALSSVAETRASLPFISDELATLISQQGLESALNQPLARILEQVQLA
                     LDNAQEKPDVIYLTGGSARSPLIKKALTEQLPGIPIAGGDDFGSVTAGLARWAEVVFR
                     "
     gene            complement(11526..13466)
                     /gene="yegI"
                     /locus_tag="NBI74_002290"
     CDS             complement(11526..13466)
                     /gene="yegI"
                     /locus_tag="NBI74_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310906.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YegI"
                     /protein_id="HCD1932614.1"
                     /translation="MKPTLYTATGECVTPGRELGKGGEGAVYDIEEFVESVAKIYHTP
                     PPALKQDKLAFMAATADAQLLNYVAWPQATLHGGRGGKVIGFMMPKVSGKEPIHMIYS
                     PAHRRQSYPHCAWDFLLYVARNIASSFATVHEHGHVVGDVNQNSFMVGRDSKVVMIDS
                     NSFQINANGTLHLCEVGVSHFTPPELQTMPSFVGFERTENHDNFGLALLIFHVLFGGR
                     HPYSGVPLISDAGNALETDIAHFRYAYASDNQRRGLKPPPRSIPLSMLPGDVEAMFQQ
                     AFTESGVATGRPTAKAWVAALDLLRQQLKKCTVSAMHVYPSHLTDCPWCALDNQGVIY
                     FIDLGEEVITTGGDFVLARVWAMVMASVAPPALQLPLPDHFQAAGRLLPSGLLRREYI
                     ILIEIALSALSLLLCGLQTEPRYIILVPVLAAIWIIGSLTSKAYKAEVQRRREALNRA
                     KMDYDHLVSQIQQLGGLEGFIAKRAMLEKMKDEILGLPEEEKRDLAALHDTARERQKQ
                     KFLEGFFIDVASIPGVGPARKAALRSFGIETAADVTRRSVKQVRGFGDHLTQAVIDWK
                     ASCERRFVFRPNEAVTPADRQAVMAKMAAKRHRLESALTVGATELQRFRLQAPARTMP
                     LMEPLRQAAEKLAQAQADLSRC"
     gene            complement(13463..14224)
                     /gene="pphC"
                     /locus_tag="NBI74_002291"
     CDS             complement(13463..14224)
                     /gene="pphC"
                     /locus_tag="NBI74_002291"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase PphC"
                     /protein_id="HCD1932615.1"
                     /translation="MSWRLVYASAVGTSHISADLPCQDACQMQVAWLNDQQPLLSVFV
                     ADGAGSASQGGEGAMLAVNEAMAYMSQKVQGGELGLNDVLATDIVLTIRQRLFAEAEA
                     KELAVRDFACTFLGLISSANGTLIMQIGDGGVVVDLGHGLQLPLTPMVGEYANMTHFI
                     TDEDAVSRLDTFTSTERAHKVAAFTDGIQRLVLNMLDNSPHMPFFTPFFNGLASATQE
                     QLDLLPELLKQFLSSPAVNERTDDDKTLALALWLP"
     gene            complement(14221..14880)
                     /locus_tag="NBI74_002292"
     CDS             complement(14221..14880)
                     /locus_tag="NBI74_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HCD1932616.1"
                     /translation="MSEQITFATSDFASNPEPRCPCILLLDVSGSMNGRPINELNAGL
                     VTFRDELLADSLALKRVELGIVTFGPVHVEQPFTSAANFFPPILFAQGDTPMGAAITK
                     ALDMVEERKREYRANGISYYRPWIFLITDGAPTDEWQAAANKVFQGEEDKKFAFFTIG
                     VQGADMKTLAQISVRQPLSLQGLQFRELFSWLSSSLRSVSRSTPGTEVVLEAPKGWTS
                     V"
     gene            complement(15102..15161)
                     /gene="ibsA"
                     /locus_tag="NBI74_002293"
     CDS             complement(15102..15161)
                     /gene="ibsA"
                     /locus_tag="NBI74_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsA"
                     /protein_id="HCD1932617.1"
                     /translation="MMKHVIILVILLVISFQAY"
     gene            complement(15434..15490)
                     /locus_tag="NBI74_002294"
     CDS             complement(15434..15490)
                     /locus_tag="NBI74_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HCD1932618.1"
                     /translation="MMKLLIIVVLLVISWPAY"
     gene            complement(15762..15818)
                     /locus_tag="NBI74_002295"
     CDS             complement(15762..15818)
                     /locus_tag="NBI74_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HCD1932619.1"
                     /translation="MMKWIIIVVLLVISFPAY"
     gene            complement(16090..16146)
                     /locus_tag="NBI74_002296"
     CDS             complement(16090..16146)
                     /locus_tag="NBI74_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HCD1932620.1"
                     /translation="MMKILIIVVLLVISYPAY"
     gene            16425..17672
                     /gene="mdtA"
                     /locus_tag="NBI74_002297"
     CDS             16425..17672
                     /gene="mdtA"
                     /locus_tag="NBI74_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter subunit MdtA"
                     /protein_id="HCD1932621.1"
                     /translation="MKGSYKSRWVIVIVVVIAAIAAFWFWQGRNDSQSAAPGATKQAQ
                     QSPAGGRRGMRAGPLAPVQAATAVEQAVPRYLTGLGTITAANTVTVRSRVDGQLMALH
                     FQEGQQVKAGDLLAEIDPSQFKVALAQAQGQLAKDKATLANARRDLARYQQLAKTNLV
                     SRQELDAQQALVSETEGTIKADEASVASAQLQLDWSRITAPVDGRVGLKQVDVGNQIS
                     SGDTTGIVVITQTHPIDLVFTLPESDIATVVQAQKAGKPLVVEAWDRTNSKKLSEGTL
                     LSLDNQIDATTGTIKVKARFNNQDDALFPNQFVNARMLVDTEQNAVVIPTAALQMGNE
                     GHFVWVLNSENKVSKHLVTPGIQDSQKVVIRAGISAGDRVVTDGIDRLTEGAKVEVVE
                     AQSATTPEDKATSREYAKKGARS"
     gene            17672..20794
                     /gene="mdtB"
                     /locus_tag="NBI74_002298"
     CDS             17672..20794
                     /gene="mdtB"
                     /locus_tag="NBI74_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtB"
                     /protein_id="HCD1932622.1"
                     /translation="MQVLPPSSTGGPSRLFIMRPVATTLLMVAILLAGIIGYRALPVS
                     ALPEVDYPTIQVVTLYPGASPDVMTSAVTAPLERQFGQMSGLKQMSSQSSGGASVITL
                     QFQLTLPLDVAEQEVQAAINAATNLLPSDLPNPPVYSKVNPADPPIMTLAVTSTAMPM
                     TQVEDMVETRVAQKISQISGVGLVTLSGGQRPAVRVKLNAQAIAALGLTSESVRTAIT
                     GANVNSAKGSLDGPSRAVTLSANDQMQSAEEYRQLIIAYQNGAPIRLGDVATVEQGAE
                     NSWLGAWANKEQAIVMNVQRQPGANIISTADSIRQMLPQLTESLPKSVKVTVLSDRTT
                     NIRASVDDTQFELMMAIALVVMIIYLFLRNIPATIIPGVAVPLSLIGTFAVMVFLDFS
                     INNLTLMALTIATGFVVDDAIVVIENISRYIEKGEKPLAAALKGAGEIGFTIISLTFS
                     LIAVLIPLLFMGDIVGRLFREFAITLAVAILISAVVSLTLTPMMCARMLSQESLRKQN
                     RFSRASEKMFDRIIAAYGRGLAKVLNHPWLTLSVALSTLLLSVLLWVFIPKGFFPVQD
                     NGIIQGTLQAPQSSSFANMAQRQRQVADVILQDPAVQSLTSFVGVDGTNPSLNSARLQ
                     INLKPLDERDDRVQKVIARLQTAVDKVPGVDLFLQPTQDLTIDTQVSRTQYQFTLQAT
                     SLDALSTWVPQLMEKLQQLPQLSDVSSDWQDKGLVAYVNVDRDSASRLGISMADVDNA
                     LYNAFGQRLISTIYTQANQYRVVLEHNTENTPGLAALDTIRLTSSDGGVVPLSSIAKI
                     EQRFAPLSINHLDQFPVTTISFNVPDNYSLGDAVQAIMDTEKTLNLPVDITTQFQGST
                     LAFQSALGSTVWLIVAAVVAMYIVLGILYESFIHPITILSTLPTAGVGALLALMIAGS
                     ELDVIAIIGIILLIGIVKKNAIMMIDFALAAEREQGMSPREAIYQACLLRFRPILMTT
                     LAALLGALPLMLSTGVGAELRRPLGIGMVGGLIVSQVLTLFTTPVIYLLFDRLALWTK
                     SRFARHEEEA"
     gene            20795..23872
                     /gene="mdtC"
                     /locus_tag="NBI74_002299"
     CDS             20795..23872
                     /gene="mdtC"
                     /locus_tag="NBI74_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtC"
                     /protein_id="HCD1932623.1"
                     /translation="MKFFALFIYRPVATILLSVAITLCGILGFRMLPVAPLPQVDFPV
                     IMVSASLPGASPETMASSVATPLERSLGRIAGVSEMTSSSSLGSTRIILQFDFDRDIN
                     GAARDVQAAINAAQSLLPSGMPSRPTYRKANPSDAPIMILTLTSDTYSQGELYDFAST
                     QLAPTISQIDGVGDVDVGGSSLPAVRVGLNPQALFNQGVSLDDVRTAISNANVRKPQG
                     ALEDGTHRWQIQTNDELKTAAEYQPLIIHYNNGGAVRLGDVATVTDSVQDVRNAGMTN
                     AKPAILLMIRKLPEANIIQTVDSIRARLPELQSTIPAAIDLQIAQDRSPTIRASLEEV
                     EQTLVISVALVILVVFLFLRSGRATIIPAVAVPVSLIGTFAAMYLCGFSLNNLSLMAL
                     TIATGFVVDDAIVVLENIARHLEAGMKPLQAALQGTREVGFTVLSMSLSLVAVFLPLL
                     LMGGLPGRLLREFAVTLSVAIGISLLVSLTLTPMMCGWMLKASKPREQKRLRGFGRML
                     VALQQGYGKSLKWVLNHTRLVGVVLLGTIALNIWLYISIPKTFFPEQDTGVLMGGIQA
                     DQSISFQAMRGKLQDFMKIIRDDPAVDNVTGFTGGSRVNSGMMFITLKPRGERSETAQ
                     QIIDRLRKKLAKEPGANLFLMAVQDIRVGGRQANASYQYTLLSDDLAALREWEPKIRK
                     KLATLPELADVNSDQEDNGAEMNLIYDRDTMARLGIDVQAANSLLNNAFGQRQISTIY
                     QPMNQYKVVMEVDPRYTQDISALEKMFVINNEGKAIPLSYFAKWQPANAPLSVNHQGL
                     SAASTISFNLPTGKSLSDASAAIDRAMTQLGVPSTVRGSFAGTAQVFQETMNSQVILI
                     IAAIATVYIVLGILYESYVHPLTILSTLPSAGVGALLALELFNAPFSLIALIGIMLLI
                     GIVKKNAIMMVDFALEAQRHGNLTPQEAIFQACLLRFRPIMMTTLAALFGALPLVLSG
                     GDGSELRQPLGITIVGGLVMSQLLTLYTTPVVYLFFDRLRMRFSRKPKQAVTE"
     gene            23873..25288
                     /locus_tag="NBI74_002300"
     CDS             23873..25288
                     /locus_tag="NBI74_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCD1932624.1"
                     /translation="MTDLPDSTRWQLWIVAFGFFMQSLDTTIVNTALPSMAQSLGESP
                     LHMHMVIVSYVLTVAVMLPASGWLADKVGVRNIFFTAIVLFTLGSLFCALSGTLNELL
                     LARALQGVGGAMMVPVGRLTVMKIVPREQYMAAMTFVTLPGQVGPLLGPALGGLLVEY
                     ASWHWIFLINIPVGIIGAIATLMLMPNYTMQTRRFDLSGFLLLAVGMAVLTLALDGSK
                     GTGLSPLAIAGLVAVGVVALVLYLLHAQNNNRALFSLKLFRTRTFSLGLAGSFAGRIG
                     SGMLPFMTPVFLQIGLGFSPFHAGLMMIPMVLGSMGMKRIVVQVVNRFGYRRVLVATT
                     LGLSLVTLLFMTTALLGWYYVLPFVLFLQGMVNSTRFSSMNTLTLKDLPDNLASSGNS
                     LLSMIMQLSMSIGVTIAGLLLGLFGSQHVSVDSGTTQTVFMYTWLSMASIIALPAFIF
                     ARVPNDTHQNVAISRRKRSAQ"
     gene            25285..26688
                     /gene="baeS"
                     /locus_tag="NBI74_002301"
     CDS             25285..26688
                     /gene="baeS"
                     /locus_tag="NBI74_002301"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     BaeS"
                     /protein_id="HCD1932625.1"
                     /translation="MKFWRPGITGKLFLAIFATCIVLLITMHWAVRISFERGFIDYIK
                     HGNEQRLQMLGDALGEQYAQHGNWRFLRNNDRFVFQILRSLEHDNNEDKPGPGMPPHG
                     WRTQFWVVDQNNKVLVGPRAPVPPDGTRRPIMVNGAEVGAVIASPVERLTRNTDINFD
                     RQQRQTSWLIVALSTLLAALATFPLARGLLAPVKRLVDGTHKLAAGDFTTRVAPTSED
                     ELGRLAEDFNQLASTLEKNQQMRRDFMADISHELRTPLAVLRGELEAIQDGVRKFTPE
                     TLASLQAEVGTLTKLVDDLHQLSMSDEGALAYQKAPIDLIPLLEVAGGAFRERFASRG
                     LKLQFSLPDSITVFGDRDRLMQLFNNLLENSLRYTDSGGSLKISAEQHDKTVRLTFAD
                     SAPGVSDDQLQKLFERFYRTEGSRNRASGGSGLGLAICLNIVEAHNGRIIAAHSPFGG
                     LSITVELPLERDLQREV"
     gene            26685..27407
                     /gene="baeR"
                     /locus_tag="NBI74_002302"
     CDS             26685..27407
                     /gene="baeR"
                     /locus_tag="NBI74_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BaeR"
                     /protein_id="HCD1932626.1"
                     /translation="MTELPIDENTPRILIVEDEPKLGQLLIDYLRAASYAPTLISHGD
                     QVLPYVRQTPPDLILLDLMLPGTDGLTLCREIRRFSDIPIVMVTAKIEEIDRLLGLEI
                     GADDYICKPYSPREVVARVKTILRRCKPQRELQQQDAESPLIIDEGRFQASWRGKMLD
                     LTPAEFRLLKTLSHEPGKVFSREQLLNHLYDDYRVVTDRTIDSHIKNLRRKLESLDAE
                     QSFIRAVYGVGYRWEADACRIV"
     gene            27587..27919
                     /locus_tag="NBI74_002303"
     CDS             27587..27919
                     /locus_tag="NBI74_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
                     /protein_id="HCD1932627.1"
                     /translation="MAGWFELSKSSDNQFRFVLKAGNGETILTSELYTSKASAEKGIA
                     SVRSNSPQEERYEKKTASNGKFYFNLKAANHQIIGSSQMYATAQSRETGIASVKANGT
                     SQTVKDNT"
     gene            28066..29427
                     /gene="yegQ"
                     /locus_tag="NBI74_002304"
     CDS             28066..29427
                     /gene="yegQ"
                     /locus_tag="NBI74_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-hydroxyuridine modification protein YegQ"
                     /protein_id="HCD1932628.1"
                     /translation="MFKPELLSPAGTLKNMRYAFAYGADAVYAGQPRYSLRVRNNEFN
                     HENLQLGINEAHALGKKFYVVVNIAPHNAKLKTFIRDLKPVVEMGPDALIMSDPGLIM
                     LVREHFPEMPIHLSVQANAVNWATVKFWQQMGLTRVILSRELSLEEIEEIRNQVPDME
                     IEIFVHGALCMAYSGRCLLSGYINKRDPNQGTCTNACRWEYNVQEGKEDDVGNIVHKY
                     EPIPVQNVEPTLGIGAPTDKVFMIEEAQRPGEYMTAFEDEHGTYIMNSKDLRAIAHVE
                     RLTKMGVHSLKIEGRTKSFYYCARTAQVYRKAIDDAAAGKPFDTSLLETLEGLAHRGY
                     TEGFLRRHTHDDYQNYEYGYSVSDRQQFVGEFTGERKGDLAAVAVKNKFSVGDSLELM
                     TPQGNINFTLEHMENAKGEAMPVAPGDGYTVWLPVPQDLELNYALLMRNFSGETTRNP
                     HGK"
     gene            complement(29927..30244)
                     /locus_tag="NBI74_002305"
     CDS             complement(29927..30244)
                     /locus_tag="NBI74_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310918.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1932629.1"
                     /translation="MKKIAAISLISIFLMSGCAVHNDETSIGKFGLAYKSNIQRKLDN
                     QYYTEAEASLARGRISGAENIVKNDAAHFCVTQGKKMQIVKLKTEGAGLHGVARLTFK
                     CGE"
BASE COUNT         6996 a         8077 c         7946 g         7234 t
ORIGIN      
        1 ttaggttgac aatatttaat atggcgtctc cacatgcgat atttcttaaa tgacgtttta
       61 ttattaccac ttataattcg gggataatgt gaggttataa ccctcaaata atatgagata
      121 aatagtgctg caacattgca tttttgcccc gatttatcca tgatcgaatt gtgacatttg
      181 tcatacaacg aataggtttt gtacttacta tggaatggat tgccgatccg tctatctggg
      241 ctggattaat cacgctgatt gtgatcgaac tggtcctcgg cattgataac ctggtcttta
      301 ttgccatcct cgccgaaaaa ctaccgccta agcagcgcga ccgcgcacgg gttaccgggc
      361 tcctgctggc gatgttaatg cgcctgttac tgctggcgtc aatctcctgg ctggtcaccc
      421 tgactcaacc gctgttcagc ttccgctcgt ttacctttag cgcccgcgat ttaatcatgc
      481 tgtttggtgg tttcttcctg ctgttcaaag ccacgatgga gttgaacgaa cggctggaag
      541 ggaaagacag caataacccc acacaacgca aaggtgcgaa gttctggggg gtggtgacac
      601 aaattgtggt gctggacgcc atcttctcac tcgactcggt gattaccgcc gtcgggatgg
      661 tcgaccattt actggtcatg atggccgccg tggttatcgc tatcagcctg atgttgatgg
      721 ccagcaagcc gttaacccag tttgttaaca gtcacccgac gatcgttatt ctctgcttaa
      781 gcttcctgtt gatgattggc tttagcctgg tggcggaagg tttcggcttc gtcattccga
      841 aaggctacct gtacgctgcc attggtttct cggtgatgat cgaggcgctc aatcagttgg
      901 ctatctttaa ccggcgacgt tttctttccg ctaaccagac actgcgccag cggactaccg
      961 aagcggtaat gcgcctgctt agcgggcaaa aagaagatgc ggaactggac gccgaaaccg
     1021 cgtccatgct ggtggatcat ggtaaccagc agatctttaa tccgcaggaa cggcggatga
     1081 ttgagcgggt acttaatctt aaccagcgta ccgtcagcag cattatgacg tcgcgccacg
     1141 atattgagca tatcgatctc aacgcgccgg aagatgagat ccgccagtta ctggagcgaa
     1201 atcagcatac gcggcttgtc gttactgatg gcgatgacgc agaagatttg ctcggtgttg
     1261 ttcacgttat cgacctgtta caacagtcac tgcgcggcga accgctcaac ctgcgggtgt
     1321 tgattcgcca gccgctggtg ttcccggaaa ccttgccgtt gttacctgcc ctggagcagt
     1381 tccgtaatgc ccgcacacac tttgcttttg tggtggatga gtttggctcg gtggaaggga
     1441 ttgtgacatt aagtgacgtc actgaaacca ttgccggtaa cttaccgaac gaagtggaag
     1501 agatcgacgc ccgccatgat attcagaaga atgcggacgg ttcctggacg gcgaatggtc
     1561 atatgccgct ggaagatctg gtgcaatatg tgccgttgcc gctggatgaa aaacgtgaat
     1621 atcacaccat tgccgggcta ttgatggaat atttgcaacg tattccaaaa cctggcgaag
     1681 aagttcaggt ggggaattat ttgcttaaaa cgttgcaggt ggaaagccat cgcgtgcaga
     1741 aggtgcagat tattccgctg cgtaaggatg gcgagatgga gtacgaggtg tgatggtatc
     1801 ggccatttta cccaatgctg cgtaacgcct tataacttca gcaatgaagc gttgtctgtg
     1861 gtacatggac atcacagcgg gggtgtgggt ccggctgtaa atcgccgaat cgtttcctgt
     1921 aggccggggc agcccacatg gatgtgggct gagggcgcgt tttacaggga tgttacctcg
     1981 cgcccgtacc cgatagccga aaggaataag atgagggaac cgcgccagcg gcgatttacc
     2041 gccgggagcc cgggttgcca gggtggtggc gattgagcca ccctggcacg ttcaccggct
     2101 tgagcgttcc atagtagcga ggaacatcaa gtgaacggaa cagccttcga tcttacatat
     2161 tcagaatata atggtgccgc cacgaacgcc tgatgcgacg ctttacgcgt cttatcaggc
     2221 ctacaggaca gctcacctgg taacctacat cttctccagc aacttcttca catctttgcc
     2281 attgcgggaa tctttattcc gctcggccca gtcgttaaga cggcgttttg cttcatcctg
     2341 tagatgttta cgcagtaact gatccacttg caggctgtaa ttaagttgct gccagttgcc
     2401 ataaacccgc agcggtactg gcgtttcttt gaggaaatca atcagtttgc tttccccgtt
     2461 ccagccaccc acgacccgaa tatcaaactg ggtgtcgcag gtttgatctg ccagattcaa
     2521 catgccttcc cctgtcagcg ccagcacagg cgattgacct tgcatgtcgt ttaacgtcac
     2581 gacgccatcc ttcaacgtca aatcggtggt aaagcggtca agacgcgtca cgttatcgaa
     2641 gttttcagcg gccttcacat caccaccatt acgttctacc gcttgctgaa tcatctgctg
     2701 gaagttcatc ccttccatgc gtgtgtcggt catttcgaca tgtgcctgtc cttgccagtt
     2761 gtggcggaat gcgtcggcat ctatgtcagc accggagaaa tcaccagcca gtgacatctt
     2821 tccggtcaac gaaatcggat agttaaacgc cttcagaatg gtgccaatct caacgttttc
     2881 cagccgtggc tggaagttta tccgcggatt tattgatgtc gcgtccaacg tgcccggcag
     2941 tgaaacctgt ccaccgttaa gtttgccctg cagttgagta atttccagca aacccgactt
     3001 gttggtcatt tgcgtggcaa catctgtaaa attcattccg cgccagcgca cgttactggc
     3061 ctgcaacaaa atatcagccg taaagccttg cagtccctga taggccggtt catcaatacg
     3121 cgaagaaatg accgggcgcg gcaacgttga ttggctctgc ccttgctgcg cggcaccgtt
     3181 ctcaccgttc gctgtttcat taagggggat gaggttgtca agattcagtt gcgggaattg
     3241 cagtctcagc tgccattccg gtttctctgt caacgtgacc tgtgcttgcc cgctcagcgt
     3301 actgtcattg gcggtcaaac taatttggtt aaatgaaagg cgtttatgtg actcctgcca
     3361 ctggacctgg aaactcccct gcccctgaat accttgtttt ggtaaatcgg caccctgcaa
     3421 ctgccagtta atttgttcaa tagccgccgt taaatcatgc ggataatcag aagcatctac
     3481 tgtaccgtta agggatatcg tgagatcgcg ctgatcgcga ttaacccgcc cggagaactc
     3541 aaatgagcca cgatgttggg gatcttgttc catttgcagg cggatattgc ggattgtcac
     3601 ctgctcgtca tcttcatgct ggaacaccag aacgctgtcc gccaccttaa gactggatat
     3661 atcaaacgac catccgcgat cgtctgacag atccggcaag gtattgtcgc gcggtgcaac
     3721 cggagcgtct tcactgcgca ccgcttccgt ctgcggcgtc agttggatca ctgccccttt
     3781 tagcatcacc tgcttaacgc tcagttgatg actcagtagt ggtaaaagcg ccacgtccag
     3841 acgcatgttg tcggcgcgaa ccagtggctg gcttgcgccc tgggcggtga gagacattcg
     3901 cccggagagg atactaagct gcggccagac gtgccaacgc agtggcccgt cgagctgcaa
     3961 ttgataaccg ctacgtgcag caacttgctt gaccatatag tcgcggaaat cattcggatt
     4021 caccagcaac actaacgcag ataacccggc caccagcacg accaggagta tcatcagcgt
     4081 cgtcagaaat cgtctcatgg tatcctcaat gggcctgaat tagtctttat cgattcggct
     4141 ggctaccgcg ccctgctggt tgcgatattt cgcatcttca cggcggttgt aaggtcgcgc
     4201 cgccgggccg gagagcggct caaagctcag cgcaccaatt aacatgcccg gacgcagcgc
     4261 cagcggcagc ttaccggagt tgtaaaactc cagcacaatg cagccagacc agcccggatc
     4321 gatacggtgc gcggtgacgt gcaccatcag ccccagacgc gccagtgaag aacgcccgtc
     4381 cagccagccc accagatcgg ctggcagcgt caccgactcc agcgtcaccg ccagcgccag
     4441 ctctcctggg tgaagataaa acgcctcgcc ctcgtcgagc acgatttcat cgctcatcac
     4501 gcggtcaagc gcggcgctca cttcatcttt aggaccgctc agatcgataa acgctgccgt
     4561 gtgaccacgg aaggtacgaa atttattgcc caggcgcaca tccaccgtcg cgccgttaat
     4621 acgctccact ggcggacgtg ggttgatcga caaacggcct tcatcaagcc aggcttcaat
     4681 atctcggtca cacagacgca tggcattttc tcctttcgag catcactccc ttaacgccaa
     4741 ttacgtcaag ggcataccag gttatcactg aacggtacac aatttatcaa gcttattcaa
     4801 agaactgact tattttcgct ttcaatatat cgatcgcgat gcggtttttc ccgccgcgcg
     4861 gcacgataat gtccgcatat tgtttagaag gctcaatgaa ttgcaggaac atcggacgca
     4921 cggttttttg atattgcgcc atcactgaat ccattgaacg cccacgctcg ttaacgtcac
     4981 gcttgatgcg gcgcatcaga cagatatcca gcggggtatc aacgaaaatg gagaagttaa
     5041 gttcgtcacg taaacgcgca tccgtcagca acaaaatgcc ttcgagaatg atgaccttct
     5101 tcggctcaac cgtcaccgtt tctttcatac gcgtatgttc aacatagctg taaaccggca
     5161 ggtcaattgc cgagccgcgt ttcaacgctt gtaaatgctc aagcagcagg ctgtgatcca
     5221 tcgcgctggg atggtcgtag ttggtcttaa cacgttcttc catcgacaga tggctttgat
     5281 ctttgtaata gcagtcttcg ggaattacgc cgatgtgttc atcaccgact tgctcacgta
     5341 attcacgata aagggtactg gcaataagac tcttgccgga agccgatgcg ccagcgatac
     5401 cgataatgac gcactgatga gactgatcag tcatatattt agcgacctga ttaacctgga
     5461 tgttaggaag ggggcgacga agcgccaaac gcggcaatta tagggatttc atccgcctga
     5521 taccagtcga atagcgttgc cgcgcgctca gagttaattg ttgacgaata attcccggtg
     5581 gcaaattacg ttgatcagtt ttatacaagg taaaaaatgt tatacgcagt tgcgcaaatt
     5641 atctgccttt acgtcacttt atgagcaatt cgcatataaa atgtaaaact tttgtactag
     5701 catgaacaca gaaacgaata ctgacgacct ggccttgcgg ataaagcggt aatgagcaaa
     5761 caatcacagc atgtattaat tgccctgccc cacccgctgc ttcacctggt cagtttaggt
     5821 ttagtctcgt ttatcttcac ccttttctca cttgagcttt cgcagtttgg cacccaactc
     5881 gccccactgt ggttcccgac gtccatcatg atggtggcgt tttatcgcca tgccgggcgc
     5941 atgtggccgg gaattgcgct gagctgctcg ctgggaaata tcgccgcatc catcctgctt
     6001 ttttccacca gctcgctgaa catgacctgg acgaccatca atattgttga agccgtggtc
     6061 ggggcagtgc tgctacgtaa attgctgccg tggtataacc ccttgcaaaa tctggctgac
     6121 tggctgcgtc tggcactcgg cagcgccatt attccgcctc tgttgggggg tgttctggtt
     6181 gtcctgctga cgcccggaga cgatcctctc agggcatttt tgatatgggt actgtcagaa
     6241 tccatcggcg ctctggcact ggtgccgctg ggattgttat ttaaaccaca ctatctgctg
     6301 cgccatcgca acccaaggtt gctttttgag tcgctgctca cgttagctat cacgctgacg
     6361 ttaagctggc tttcgatgct gtatctgccg tggcctttta ctttcattat tgtgctgttg
     6421 atgtggagcg ccgtgcgcct gccacgaatg gaagcctttt tgatcttcct taccacagtg
     6481 atgatggtat cgctgatgat ggccgcggat ccctctctgc ttgctacgcc gcgtacgtac
     6541 ctgatgagtc atatgccgtg gctaccgttt ttgctgatcc tgctgcccgc caacatcatg
     6601 accatggtga tgtatgcctt tcgtgcggaa cgcaaacaca tttccgaaag cgaaacccgc
     6661 tttcggaacg cgatggaata ttccgctatc ggcatggcat tagtgggcac cgagggacaa
     6721 tggctgcaat ccaacaaagc gctctgccag tttctcggtt acagtcagga agaactgcgc
     6781 ggactcacct ttcagcaact gacctggccg gaggatctca ataaagatct gcaacaggtt
     6841 gaaaagctga taagcggtga aataaacacc tattcaatgg aaaaacgcta ctacaaccgc
     6901 aatggcgatg ttgtctgggc gttgcttgcc gtctcactgg tgcgccacac ggatggcacg
     6961 ccgctctatt ttatcgctca gattgaagac attaacgagc taaaacgcac cgaacaggtg
     7021 aaccagcaac tgatggagcg catcacgctg gccaacgaag cgggcgggat tggcatctgg
     7081 gagtgggagc taaagccgaa tatttttagc tgggataagc ggatgttcga gctgtatgaa
     7141 attcctccgc atatcaaacc gaactggcag gtgtggtacg agtgcgtgct gccggaagat
     7201 cgccagcacg ccgaaaaagt gattcgcgat tcattgcaat cgcgctctcc ctttaaactg
     7261 gaatttcgca ttaccgtgaa agacggcatt cgccatatcc gcgccctcgc taaccgggta
     7321 ctgaataaag aaggcgaagt cgaacgcctg ctcggcatta atatggatat gaccgaggtt
     7381 aaacagctta acgaggcatt gtttcaggaa aaagagcgcc tgcacattac gctggattcc
     7441 atcggtgaag ccgtggtctg tattgatatg gcgatgaaaa ttacctttat gaatccggtg
     7501 gcggagaaga tgagcggctg gacgcaggaa gaagcgttag gtgttccgct cctgacggtg
     7561 ttgcatatta cttttggcga caacggacca ttaatggaga acatttacag tgccgacacc
     7621 tcacgttccg cgattgaaca agatgtggtg ttgcactgcc ggagcggcgg cagctacgac
     7681 gtgcattaca gtattacgcc gttaagtact ctggacggca gcaatattgg ttcggttctg
     7741 gtgattcagg acgtcaccga atcgcgcaaa atgctgcgcc agctgagcta cagcgcctcc
     7801 catgatgcac tgacgcatct cgccaatcgc gccagttttg agaagcaact gcgtatcctg
     7861 ctgcaaacgg taaacagtac gcaccagcga catgccctgg tgtttatcga tcttgatcgc
     7921 tttaaagcgg tgaatgacag cgccgggcac gccgctggcg acgctttact gcgcgaactg
     7981 gcgtcattga tgctgagtat gctgcgctcc agcgacgtgc tggcgcgact cggtggcgat
     8041 gaatttggtc tgctgctgcc agattgtaat gtcgaaagtg cgcgttttat cgctacacgc
     8101 attatcagcg ccgtgaatga ctatcacttt atctgggaag gacgtgtaca tcgggtaggt
     8161 gccagtgcag ggattacctt gattgatgac aacaatcatc aggcggctga agtgatgtcg
     8221 caggctgata tcgcctgtta tgcctccaaa aatggtggac ggggccgggt gacgatttac
     8281 gaaccgcagc aagttgccgc acatagcgaa cgggctgtga tgtcgcttga tgaacagtgg
     8341 cggatgatta aagagaatca gttgatgatg atcgcccacg gtgtcgcttc gccgcggatc
     8401 ccgcaagcgc gtaatttgtg gctgatttca cttaagctct ggagttgcga aggcgagatt
     8461 attgatgaac aaacatttcg tcgtagcttc agcgatccgg cacttagcca tgctcttgac
     8521 cgacgggtat tccacgattt tttccagcag gccgcaaaag cgattgccag taaaggctta
     8581 agcatcgccc tccccctttc cgttgccggt ttgagtagcg ccacgctggt gaatgaacta
     8641 attgagctgc tggaaaatag ccctctacca ccacggttat tacatctgat tattccggca
     8701 gacgcgattt tagatcacgc agaaagcgtg caaaaactgc ggctggcggg atgtcggatc
     8761 gtattcagtc aggtgggccg cgatctgcaa atcttcaact cgctgaaagc gaatatggca
     8821 gattacctgc tacttgatgg tgagttatgc gccaacgtgc agggaaattt gatggatgag
     8881 atgctgatta cgatcattca ggggcacgct cagcggctcg ggatgaagac catcgccggg
     8941 ccagtcgttt tacccttagt gatggatacg ctttctggca tcggcgtcga tctgatttat
     9001 ggcgatgtga ttgccgatgc ccaaccgctg gatttgctgg taaatagcag ttatttcgcg
     9061 attaactaag ggagcgtttg cccattgcct gatgcgacgc taacgcgtct tatcaggcct
     9121 acaaatcgct cattccccag gccggataag gcgctcgcac cgcatccggc aaccaacgtc
     9181 atgcttcgtc tggttgccag ccttccgtat accagatatg caacagcgca taagaacgcc
     9241 agggcttcca gcgttcggca taacggcgga tttgtgccgg tgtcattccc ggaaatcgct
     9301 gtttaatcag ataatcatcc ggcaggaaaa catctttcgc ctgccagcca cgtaaggcga
     9361 aataattcgc cgtccagcgc ccgatacccg gaaaagtttg cagcgttttc atcgcttgtt
     9421 ctacgtcgcc cggtattgtc attggtaagg tgccctccag cgccgcattt gccagatgaa
     9481 tcagtgcctc tgcccgtttc aacggcatac ctaacgcttt taatgcctgc gggtcggctg
     9541 cagccagccg atgaggcgtc gggaagcaga cataatcagg aaaatcatcc agcctttcgc
     9601 cataaagctg tgccactctg gcggtcaatt ttgccgccat cgccacgctc accagttggc
     9661 ctaaaatcgc ccgcacgccc tgctcaaaag catcaacact gccgggtaaa cgcaatccgg
     9721 gccgccccgc gcctaacttt cccaacgcac cgttaacaat ctgcgggtta cattgcagat
     9781 caaacaggcg gctcattttc gccagacact ctgcggcaac aggttctaaa cctgcactta
     9841 aatttatgtg cagagtatgg cgggctatat ccggaatagc agtcaccacg ccgcgatatt
     9901 cgcccaccgc cagactacgg gcataataac tgtccgcgac cgtttccaca ccgttcaccg
     9961 cacgggcggc gagaaatccc aacatccacg accagtcata cggcggctgc cagttcaggg
    10021 tatacatcgc atctccttgt taatccgctt tcagcataaa cgttattcag acgttacgct
    10081 ttgctttcat attccggttg tcgcgacggc aacatttcgc taaagtcacg ccccttcttc
    10141 accggcatgg ggattatttc gtgtttattg gttttgatta cggtacagca aactgttcag
    10201 tggcggtcat gcgcgacggt aagccgcact tgctaaaaat ggaaaacgac agcacgctgc
    10261 tgccttcaat gctttgcgcg ccaacgcgtg aagcggtaag cgaatggctg taccgccatc
    10321 atgacgttcc ggcagacgac gatgaaacgc aggcgctgtt acgtcgggcg attcgttata
    10381 accgcgaaga agatatcgat gttacggcga aaagcgtgca gttcggtctt tcctcactgg
    10441 cacagtacat tgatgatccg gaagaagtgt ggtttgtgaa atcaccaaaa tcgttcctcg
    10501 gtgccagcgg cttaaaacca cagcaggtag cgctgtttga ggatctggtc tgcgcaatga
    10561 tgttgcacat tcgccaacag gcgcaggcgc agctgccgga ggcaattact caggcggtga
    10621 ttggtcgccc gatcaacttc caggggctgg gcggtgatga agcaaacacc caggcgcaag
    10681 ggattctgga acgcgcggcg aagcgtgccg ggtttaagga tgtggtattc cagtacgagc
    10741 cggtcgcggc tgggctggat tacgaagcca ccttgcagga agaaaaacga gtgctggtag
    10801 tggatatcgg cggtggtacg actgactgtt cattgctgct gatggggccg caatggcgtt
    10861 cgcgtctcga tcgtgaagcc agcctcctgg gtcatagtgg ttgccgtatt ggtggtaacg
    10921 atctggatat cgcactggcg tttaagaacc tgatgccgtt actgggcatg gggggcgaaa
    10981 ccgaaaaagg catcgccctg ccgattctgc cgtggtggaa tgcggttgcc atcaacgacg
    11041 tacctgcgca aagtgatttc tacagtagtg ccaacggtcg tctgcttaac gatctggtac
    11101 gcgatgcccg cgaaccggaa aaagtggccc tgttacagaa agtctggcgt cagcgtttaa
    11161 gctatcgcct ggtacgtagc gcagaagaga gcaaaattgc gctttcaagc gtagcggaaa
    11221 cccgcgcctc actgccgttt atcagcgatg aactggctac gctgattagc cagcaagggc
    11281 tggaaagcgc cctcaaccag ccactggcgc ggattctgga acaggtgcaa ctggcgctgg
    11341 ataacgccca ggaaaaaccg gatgttatct atctgaccgg cggtagcgcc cgttcaccgc
    11401 tgattaaaaa agcgctgaca gaacagttgc cgggcattcc gattgcaggt ggcgatgact
    11461 ttggctccgt caccgccggg ctggcacgct gggcggaagt ggtgtttcgt taattgaaaa
    11521 ctggctcagc aacggcttaa atcagcctgc gcctgagcca gtttttctgc cgcctgacgt
    11581 aacggttcca tcaacggcat ggtccgtgct ggagcttgaa ggcggaatcg ctgcaactct
    11641 gtcgcaccga cagtcagcgc cgattccagc cgatgtcgtt tggcggccat ttttgccatt
    11701 accgcttgtc tgtctgcagg cgttaccgct tcgttcggcc tgaaaacaaa acggcgttcg
    11761 caactcgctt tccagtcgat aaccgcctgg gtcagatgat caccaaaacc cctaacttgc
    11821 ttaacgctcc gtcgggtaac gtccgctgct gtttcaatac caaaggaccg taacgccgct
    11881 ttacgcgcag ggccaacgcc gggaatagag gcaacatcaa taaaaaatcc ctccagaaac
    11941 ttctgcttct gccgttccct tgcggtgtcg tgaagtgctg ccagatcgcg cttttcttct
    12001 tccggtaacc caagaatttc gtccttcatt ttttcgagca tcgcccgttt ggcgataaaa
    12061 ccttccagcc cgcccaactg ttggatctgg ctgactaaat ggtcatagtc catttttgcg
    12121 cgattcaaag cctctcttcg tcgctggact tctgctttgt acgctttgct tgtcaggctg
    12181 ccaataatcc agatagccgc cagcacggga accaaaatga tataacgcgg ttctgtctga
    12241 aggccgcaaa gcagcagcga taacgctgac agtgcgatct caatcagaat gatgtattcg
    12301 cgccgtaaca ggcctgaagg aagcagcctg ccagcagcct ggaaatgatc gggtaatggc
    12361 agttgcaatg ctggcggtgc tactgatgcc atcaccatcg cccagactct cgccagcaca
    12421 aaatcaccgc cggtggtaat gacctcttcg ccgagatcaa taaaatagat aacgccttga
    12481 ttatccagcg cacaccacgg gcagtcagtc aaatgagagg ggtaaacatg cattgccgaa
    12541 acggtacatt tctttaactg ttggcgtagt aaatcaagtg ccgctaccca cgcttttgcc
    12601 gtcggacgcc cagttgccac gccactttcc gtgaatgcct gctgaaacat ggcttcaaca
    12661 tcgcccggta acatcgacag cggaatcgat cgtggcggcg gttttaaacc acgtcgctga
    12721 ttatctgacg cgtaggcata acggaaatgg gcaatatccg tctccagcgc attacccgca
    12781 tcagagataa gcggtacacc ggaataagga tgccgcccac caaacaagac gtgaaatatc
    12841 agcaacgcaa ggccaaaatt atcgtgattt tctgtgcgtt caaagccgac aaatgatggc
    12901 atggtttgca gctctggcgg cgtaaaatgc gacacgccga cttcgcataa atgcagtgta
    12961 ccattggcat taatctgaaa ggagttgcta tcaatcatca ccactttgct gtcgcgacct
    13021 accataaagc tgttctggtt tacgtcaccc acgacgtgcc cgtgctcgtg aaccgtagca
    13081 aaagatgaag caatattgcg cgcaacatag agtagaaaat cccacgcaca atgagggtaa
    13141 ctctggcgac gatgtgccgg gctatagatc atatgaatcg gttctttacc agaaactttt
    13201 ggcatcataa aaccgataac ttttccgcct cgtccaccgt gaagcgttgc ctgcggccag
    13261 gcgacataat tcaacaactg cgcgtcagct gtcgcagcca taaaggcaag tttgtcctgt
    13321 tttaaagcgg gaggcggcgt gtgataaatc ttggcgacgc tctcgacaaa ctcctcgata
    13381 tcataaaccg caccttcgcc gcctttgccc agttcacggc ctggcgtaac gcactcacca
    13441 gtagcagtat ataaagtggg tttcatggta accacagcgc cagtgccaac gttttatcat
    13501 catccgtacg ctcgtttacc gccgggctgg acaaaaactg ctttaacaat tcaggtaata
    13561 aatcgagttg ttcctgcgtt gctgacgcca gtccattgaa aaacggggta aagaaaggca
    13621 tatgcggaga attatccagc atatttaaca ccagtcgctg aataccatcc gtaaatgctg
    13681 caactttgtg cgcacgctca gtgctggtga aagtatccag tctggaaacg gcatcttcat
    13741 cagtaatgaa gtgcgtcata ttggcatatt cccccaccat cggcgtgagc ggcagttgca
    13801 aaccgtgacc caaatctacc accacgccgc catcgccaat ctgcataatc aaggtgccat
    13861 tagctgatga aatcaatccc aaaaacgtgc aggcaaaatc gcgaaccgct aattctttgg
    13921 cttccgcctc ggcaaaaagt cgctgacgaa tggtcagcac aatgtccgtt gcgaggacat
    13981 cattaagccc caattccccg ccctgcactt tttgcgacat ataagccatc gcttcattga
    14041 cggcgagcat cgcaccttcg cctccctgcg aggcgctacc tgcgccatca gcgacaaata
    14101 ccgacaataa tggctgctgg tcatttagcc aggcaacctg catctggcag gcatcctggc
    14161 aaggcaagtc cgcactgatg tgcgacgttc caacggctga tgcatagacc agtcgccagc
    14221 tcacactgac gtccagcctt ttggcgcttc cagcacaact tccgttcccg gcgtggagcg
    14281 ggaaaccgaa cgcagcgaac tggataacca gctaaacagt tcacggaatt gtaatccctg
    14341 cagagacaaa ggctgacgga cgctgatttg ggccagcgtc ttcatatccg ccccctgaac
    14401 accgatggta aagaaggcaa acttcttatc ttcttccccc tgaaacactt tgttggcggc
    14461 agcctgccac tcatcggttg gtgcgccatc ggtgatcagg aaaatccacg gacgataata
    14521 ggaaatacca ttggcacgat attcacgttt ccgctcctca accatatcca gggctttggt
    14581 aatggcagcg cccattggtg tatcgccctg ggcaaacagg atgggcggga aaaaattagc
    14641 ggcgctggta aagggttgtt ccacatgcac cgggccgaac gtcacaatgc cgagttccac
    14701 tctttttaag gccagcgaat cggcaagcag ctcatcgcga aaggtaacca atccggcgtt
    14761 aagttcatta atgggtctac cgttcataga accagaaaca tccagcagca aaatgcaggg
    14821 gcaacgtggt tctgggttac tggcaaaatc gctggtggca aacgtgattt gttcgctcat
    14881 aagaaatcct ttcttagaga attattgagg tccggtatca taccgattgc aataatgaaa
    14941 taagagcaaa cgaagaaact ttcagaatta ggcccggcgg cgcagaaaat tttgctattc
    15001 gcctcgcaag aacggagagc cgtcgattga cactaagggc ggagtgacat aatttcagga
    15061 gtgagggtta gggagaggtt tcccccctcc ccctggtgtt cttagtaagc ctggaagcta
    15121 atcactaaga gtatcaccag tatgatgacg tgcttcatca taaccctttc cttattaaaa
    15181 gccctcttct ccgggagagg gtttcccgtt tcagcgtccc gctgaaatcg tcggcttacc
    15241 tcctttcgcc ctgaatgcag tctatcgcta acgcgtgaac aaaatagttt cctctgtgat
    15301 tattgcgtaa cgactcgcaa agaatatttg ctggcaggat cgcagactac aaagcctgcg
    15361 gattgacaat cttatcgtga aggcataatt tcaggagtga gggtagagcg gggtttcccc
    15421 cgccctggta gtcttagtaa gctggccagc taataactaa gagcaccacg atgatgagta
    15481 gcttcatcat gaccctttcc ttatttaagg ccccttcctc gggaggggct ttcccgtttc
    15541 agcgtcccgc tgaaatcggc ggcctacctc ctttcgccat gcaagcagtc tatcgctaac
    15601 ttgcggataa aatagtttca catgtgatta ttgcgtaacg tctcgcaaag aatatttgct
    15661 ggcaggatcg cagactacaa agcctgcgga ttgacaatct gattgtgaag gcatactttc
    15721 aggagtgagg gtagagcggg gtttcccccg ccctggtagt cttagtaagc cgggaagctt
    15781 atgactaaga gcaccacgat gatgatccac ttcatcatga ccctttcctt atttaaggcc
    15841 ccttcctcgg gaggggcttt cccgtttcag cgtcccgctg aaatcttcgg cttacctcct
    15901 ttcgccctgt atgcagtcta tcgctgactt gcggataaaa tagtttcaag tgtgattatt
    15961 gcgtaacgac tcgcaaagaa tatttgctgg caggatcgca gactgcaaag tctgcggatt
    16021 gacaattgga ccgtgaaggc atactttcag gagtgagggt agagcggggt ttcccccgcc
    16081 ctggtagtct tagtatgctg ggtagctaat cactaagagc accacgatga tgagtatctt
    16141 catcatgacc ctttccttat ttaaggcccc ttcctcggga ggggctttcc cgtttcagcg
    16201 tcccgctgaa atcgtcggct tatctccttt cgccatgcaa gcagtctatc gctaacgcgt
    16261 agataaaata gtttcatgtg ttattactgg atgcgtgctc gcaaaagtgc ccgtcattca
    16321 gacgattccc gacagtgttt cataattcct ccatttttct cccttattgg ctggctacac
    16381 tagtatcatt ccgcgaaacg tttcaggaag agaaactctt aacgatgaaa ggcagttata
    16441 aatcccgttg ggtaatcgta atcgtggtgg ttatcgccgc catcgccgca ttctggttct
    16501 ggcaaggccg caatgactcc cagagtgcag ccccaggggc gacgaaacaa gcgcagcaat
    16561 cgccagcggg tggtcgccgt ggtatgcgtg ccggcccatt agctccggtt caggcggcga
    16621 ccgcagtaga acaggctgtt ccgcgttacc tcaccgggct tggcaccatt accgccgcta
    16681 atactgttac ggtgcgcagc cgcgtggacg gtcaactgat ggcgttacat ttccaggaag
    16741 gccagcaggt caaagcaggc gatttactgg cagaaattga cccaagccag ttcaaagttg
    16801 cattagcaca agcccagggc caactggcaa aagataaagc cacgcttgcc aacgcccgcc
    16861 gtgacctggc gcgttatcaa caactggcaa aaaccaatct cgtatctcgt caggaactgg
    16921 atgcccaaca ggcgctggtc agtgaaaccg aaggcaccat taaggctgat gaagcaagcg
    16981 tcgccagcgc gcaattgcaa ctcgactgga gtcgcattac cgcaccagtc gatggtcgcg
    17041 ttggtcttaa gcaagttgat gttggtaacc aaatctccag tggcgacacc accgggattg
    17101 tggtgatcac ccaaacacat cctatcgatc tggtctttac tctgccggaa agcgatatcg
    17161 ccaccgttgt acaggcacaa aaagccggaa aaccgctggt ggtagaagcc tgggatcgca
    17221 ccaactcgaa gaagttaagt gaaggcacgc tgttaagtct cgataaccaa atcgatgcca
    17281 ctaccggtac gattaaagtg aaagcacgct ttaataatca ggatgatgcg ctgtttccca
    17341 atcagtttgt taacgcgcgc atgttagtcg acaccgaaca aaacgccgtg gtgatcccca
    17401 ccgccgctct gcaaatgggc aacgaaggcc attttgtctg ggtactgaat agcgaaaaca
    17461 aagtcagcaa acatctggtg acaccgggca ttcaggacag tcagaaagtg gtgatccgcg
    17521 caggtatttc tgcgggcgac cgcgtagtga cggacggaat tgatcgcctg accgaagggg
    17581 cgaaagtgga agtcgtggaa gcccagagcg ccactactcc ggaagataaa gccaccagcc
    17641 gcgaatacgc gaaaaaagga gcgcgctcct gatgcaggtg ttacccccga gcagcacagg
    17701 cggcccgtca cgcctgttta ttatgcgtcc tgtcgccacc acgctgctga tggtggcgat
    17761 cttactcgcc gggattatcg gttatcgcgc cctgcccgtt tcggcgctgc cggaagtgga
    17821 ctatccaacc attcaggtgg tcacgcttta tccaggtgca agcccggatg tcatgacctc
    17881 tgccgttacc gcgccgctcg aacgccagtt cgggcagatg tctggcctga aacagatgtc
    17941 gtcgcaaagt tccggcggtg cgtcagttat cactttacag ttccagctaa cattgccgct
    18001 ggatgtcgcc gagcaggaag tgcaggccgc gattaacgct gcgaccaacc tgctaccgag
    18061 cgatctgcct aacccgccgg tttacagcaa agtgaacccg gcagatccac cgatcatgac
    18121 gctcgctgtc acctcaaccg ccatgccgat gacgcaggtg gaagatatgg tggaaacccg
    18181 cgtcgcgcag aaaatctcgc agatttccgg cgtagggctg gtgacacttt ccggcgggca
    18241 gcgtccggcg gtacgcgtaa aactcaacgc tcaggcaatt gccgctctcg gcctgaccag
    18301 cgaaagtgtg cgcaccgcca ttactggcgc gaacgttaac tcagcgaaag ggagccttga
    18361 cggcccttct cgcgcggtca cgctgtcagc gaatgaccag atgcaatccg ccgaagagta
    18421 tcgccagcta atcatcgcct accagaacgg cgcaccaatt cgtcttggcg atgtcgcaac
    18481 cgtagagcaa ggagcagaaa acagctggct cggcgcgtgg gcgaacaaag aacaggccat
    18541 tgtgatgaat gttcagcgcc agcccggtgc taacattatc tccaccgccg acagcatccg
    18601 gcagatgcta ccacagctca ccgagagtct gccgaaatcg gtgaaggtga cggtactttc
    18661 tgaccgtacc accaatatcc gcgcatcggt cgacgatacc cagtttgagc tgatgatggc
    18721 tatcgcgctg gtagtcatga ttatctacct gtttttgcgc aatattccgg cgaccatcat
    18781 tcccggtgtt gctgtaccgc tgtcgttaat tggcaccttt gcggtcatgg tgtttctcga
    18841 tttttcaatc aataacctga cgttgatggc gttaactatc gccaccggat tcgtggtcga
    18901 tgacgccatc gtggtgatcg aaaacatttc ccgctatatc gaaaaaggcg aaaaaccgct
    18961 ggcagccgcg ctcaaaggcg cgggtgaaat cggctttacc attatctcgc tgaccttctc
    19021 gctgattgcg gtattgatcc ccctgctgtt tatgggcgat atcgtcgggc gactgttccg
    19081 cgaatttgct attaccctgg cagtcgcgat tttgatctca gcagtggtgt cgctgaccct
    19141 gacaccgatg atgtgcgcgc ggatgctcag ccaggagtcg ctgcgtaaac agaaccgctt
    19201 ctcccgtgcc tcggagaaaa tgttcgacag gataatcgcc gcctatggtc gtggactggc
    19261 gaaagtgctg aatcatccgt ggctgacctt aagcgtagcg ctcagcacgc tgctgcttag
    19321 cgtgctgctg tgggtgttca ttccgaaagg tttcttcccg gtacaggaca acggcataat
    19381 tcagggcact ttgcaggcac cgcaatccag ctcctttgcc aatatggccc agcggcaacg
    19441 ccaggtcgcg gacgtgattt tgcaggatcc ggcagtgcaa agtttgacct catttgttgg
    19501 cgttgatggc actaacccgt cgctgaacag tgcgcgttta cagatcaatc tcaaaccact
    19561 ggatgaacga gatgaccggg tgcaaaaagt catcgcccgt ctgcaaacgg cggtggataa
    19621 agtgccgggc gtagatctct tcctgcaacc aacgcaggac ctgactattg atactcaggt
    19681 cagccgcacc cagtaccagt ttaccttgca ggccacgtca ctggatgcgc tcagtacctg
    19741 ggtgccacag ttgatggaaa aactccagca gctgccacag ctttctgatg tctccagcga
    19801 ctggcaggac aaagggctgg tggcgtatgt caatgtcgac agggacagcg ccagccgtct
    19861 ggggatcagc atggcggatg tcgataacgc cctgtacaac gcgtttggtc agcgactgat
    19921 ttccactatt tatactcagg ctaaccagta tcgcgtggtg ctggaacaca acaccgaaaa
    19981 taccccaggc ctcgcggcgc tggataccat tcgcctgacc agcagcgacg gtggcgtggt
    20041 gccgttaagc tcaattgcca aaattgagca gcgttttgcg ccgctttcca tcaaccatct
    20101 ggatcagttc ccggtaacga ctatctcctt taacgtgccg gataactatt cgctgggcga
    20161 tgcggtgcag gcgattatgg acacagagaa gacgctgaat ctgccggtgg atatcaccac
    20221 gcagttccag ggcagcaccc tcgccttcca gtcggcgctg ggcagcactg tctggctgat
    20281 tgtcgcggcg gtggtggcga tgtatatcgt gctcggcatt ctgtacgaga gctttattca
    20341 cccgatcacc attctctcga cgctacccac cgcaggggtc ggcgcgctgc tggcgttaat
    20401 gattgccggt agcgaactgg atgtgattgc cattatcggc attattttgc tgatcggtat
    20461 cgtgaagaag aacgccatca tgatgatcga cttcgcgctg gctgctgagc gcgagcaagg
    20521 catgtcgccg cgcgaagcta tttaccaggc ctgtctgttg cgttttcgtc cgatcctgat
    20581 gaccactctg gcggctctgc ttggtgcgct gccgctgatg ttgagtaccg gggtcggcgc
    20641 ggaactgcgt cgtccgttag gtatcggcat ggtcggcggt ctgattgtca gccaggtgct
    20701 gacgctgttt accacgccgg tgatttatct gttgttcgac cgtctggcat tgtggaccaa
    20761 aagccgtttt gcccgtcatg aagaggaggc gtaagtgaag ttttttgccc tcttcattta
    20821 ccgcccggtg gcgacgattt tactgtcggt cgccattacc ctgtgcggca tactgggttt
    20881 ccgtatgctg ccagtcgccc cgctgccgca ggtcgatttt ccggtgatca tggtcagcgc
    20941 ctcgctgccc ggtgcgtcac cagaaacaat ggcgtcttcc gttgccacgc cgctggagcg
    21001 ctcacttggg cgcattgccg gagtcagcga aatgacctcc agcagttcgc tcggcagcac
    21061 gcgcattatt ttgcagtttg attttgaccg ggatatcaac ggcgcagcgc gcgatgtgca
    21121 ggcggcgatc aacgccgcgc aaagtctgct acccagcggg atgcccagcc gtccgaccta
    21181 tcgcaaagcg aacccatcgg atgcgccaat tatgatcctc acgctgacat ccgatactta
    21241 ttcgcagggt gaactgtacg atttcgcctc gacacagctg gctccgacga tttcgcaaat
    21301 cgacggtgtc ggtgatgtcg atgttggcgg cagctcgctg cccgccgtac gcgtcgggct
    21361 gaatccgcag gcgctgttta atcagggcgt ctcgctggac gacgtacgca ccgccatcag
    21421 caatgccaac gtgcgtaaac cgcagggggc gctggaagat ggtactcacc gctggcagat
    21481 ccagactaac gatgaactga aaaccgccgc tgaatatcag ccgctgatta ttcattacaa
    21541 caacggcgga gcggttcgtc tgggcgatgt ggcgacggtg accgactcag tacaggatgt
    21601 gcgcaacgcc gggatgacca acgccaaacc ggctattttg ctgatgatcc gcaagctgcc
    21661 ggaagccaat attatccaga cggtagacag catccgcgcc cgtctgccgg aattacaatc
    21721 caccatcccg gcagcaattg atctgcaaat tgcccaagat cgctccccta ctatccgcgc
    21781 gtcgctggaa gaggttgagc agacgctggt aatctcagtt gcactggtga ttctggtggt
    21841 gtttttgttc ctgcgttcgg gtcgcgccac cattattccc gccgttgcgg tgccggtttc
    21901 gctgattggt acgtttgcgg cgatgtacct gtgtggtttc agtctcaata atctttcgtt
    21961 aatggcgctc actatcgcca ctggctttgt ggtggatgac gccatcgtgg tgctggaaaa
    22021 cattgcccgt catctggaag cggggatgaa accgctacag gccgcgctac aaggtactcg
    22081 cgaagtcggc tttacggtgc tgtcgatgag tctgtcactg gtggcggtgt tcctgccgct
    22141 gctgttgatg ggcggattgc cgggtcgcct gttacgcgag tttgccgtga cgctttctgt
    22201 cgccattggt atttcgctgc tggtttccct gacgttaacg ccgatgatgt gcggctggat
    22261 gctaaaagcc agcaagccgc gcgagcaaaa gcgactgcgt ggctttggtc gcatgttggt
    22321 agccctgcaa caaggctatg gcaagtcgct gaaatgggtg ctcaatcata cccgtctggt
    22381 gggcgtggtg ctgcttggca ccattgcgct gaatatctgg ctgtatatct cgatcccgaa
    22441 aaccttcttc ccggagcagg acaccggcgt actgatgggt gggatacagg ctgaccagag
    22501 tatttcgttt caggcgatgc gcggtaagtt gcaggacttc atgaaaatta tccgtgacga
    22561 tccggcggtg gataatgtca ccggctttac cggcggttca cgggtgaaca gtggaatgat
    22621 gtttatcacc cttaaaccac gcggcgagcg gagcgaaacg gcccaacaga ttatcgatcg
    22681 cttgcgtaaa aaactggcta aagaaccggg agcgaatctg ttcctgatgg cggtacagga
    22741 tattcgcgtt ggcggacgtc aggctaacgc cagttaccag tacacgttgt tatccgacga
    22801 cctggcggca ctgcgcgaat gggagccgaa aatccgcaaa aaactggcga cattgccaga
    22861 gctggctgat gttaactccg atcaggaaga taatggcgcg gagatgaacc ttatttacga
    22921 tcgcgacacc atggcgcgat tgggaattga cgtgcaagcc gccaacagcc tgttaaataa
    22981 cgccttcggt cagcggcaaa tctcgaccat ttaccagccg atgaaccagt acaaagtggt
    23041 gatggaagtg gatccgcgct atacccagga catcagtgca ctggaaaaaa tgttcgttat
    23101 caataacgaa ggaaaagcga tcccgctgtc gtatttcgct aaatggcaac cggcgaacgc
    23161 tccactgtcg gtgaatcatc agggattatc ggcggcctcg accatttcgt tcaacctgcc
    23221 gaccgggaaa tcgctctcgg acgccagtgc ggcgatcgat cgcgcaatga cccagcttgg
    23281 tgtgccttcg acggtgcgcg gcagttttgc cggaacagcg caggtgttcc aggagacgat
    23341 gaactcacag gtgatcctga ttatcgccgc catcgccacg gtatatatcg tactgggtat
    23401 cctttacgag agttatgtgc atccactgac gattctctcc accctacctt cggcgggcgt
    23461 cggagcgctg ttggcgctgg agctgttcaa tgccccgttc agcctaattg cgctgatagg
    23521 gatcatgtta ttaatcggca tcgtgaagaa aaacgccatt atgatggtcg attttgcgct
    23581 tgaagcccaa cggcacggta acctgacgcc gcaggaagct attttccagg cctgtctgct
    23641 gcgttttcgc ccgattatga tgactaccct ggcggcgctg tttggtgcgc tgccgctggt
    23701 attgtcgggc ggtgacggct cggagttgcg gcaaccattg gggatcacca ttgtcggcgg
    23761 tctggtcatg agccagcttc tgacgctgta taccacgccg gtggtgtatc tctttttcga
    23821 ccgtctgcgg atgcgttttt cgcgtaaacc taaacaagcg gtaaccgagt aaatgacaga
    23881 tcttcccgac agcacccgct ggcaattgtg gattgtggct ttcggcttct ttatgcagtc
    23941 gctggacacc accatcgtta acaccgcgct tccctcaatg gcgcaaagcc tcggggaaag
    24001 tccgttgcat atgcacatgg tcattgtctc ttatgtactg accgtggcgg tgatgctgcc
    24061 cgccagcggc tggctggcgg acaaagtcgg cgtgcgcaat attttcttta ccgccatcgt
    24121 gctgtttact ctcggttcac tgttttgcgc gctttccggc acgctgaacg aactgttgct
    24181 ggcacgcgcg ttacagggcg ttggcggcgc gatgatggtg ccggtcggca ggttgacggt
    24241 gatgaaaatc gtaccgcgcg agcaatatat ggcggcgatg acctttgtca cgctgcccgg
    24301 tcaggtcggg ccgctgcttg gcccggcgct cggcggtctg ctggtggagt acgcatcgtg
    24361 gcactggatc tttttgatca acattccggt gggaattatt ggcgcgatcg ccacactgat
    24421 gttaatgccg aactacacca tgcagacgcg gcgctttgat ctctccggct ttctgttgct
    24481 ggcggttggc atggcggtat taaccctggc gctggacggc agtaaaggta caggtttatc
    24541 gccgctggcg attgctggcc tggtcgcagt tggcgtggta gcactggtgc tttatctgct
    24601 gcacgcccaa aataacaacc gcgccctgtt cagtctgaaa ctgttccgca ctcgtacctt
    24661 ttcgctgggc ctggcgggga gctttgccgg acgtattggc agtggcatgt tgccctttat
    24721 gacaccggtt ttcctgcaaa ttggcctcgg tttctcgccg tttcatgccg gactgatgat
    24781 gatcccgatg gtactcggca gcatgggaat gaagcgaatt gtggtacagg tagtgaatcg
    24841 ctttggttat cgtcgggtac tggtggcgac cacgctgggc ctgtcgctgg tcaccctgtt
    24901 gtttatgact accgctctgc tgggctggta ctacgttttg ccgttcgtcc tgtttttaca
    24961 aggaatggtc aactcgacac gtttctcctc catgaacacc ctgacgctga aagatctccc
    25021 ggacaacctg gcgagcagcg gcaacagtct gctgtcgatg attatgcaat tgtcgatgag
    25081 tattggcgtc accatcgccg ggctgttgct gggacttttt ggttcgcagc atgtcagcgt
    25141 cgacagcggc accacacaaa ccgtctttat gtacacctgg cttagcatgg cgtctatcat
    25201 cgcccttccg gcgttcatct ttgccagagt gccgaacgat acgcatcaaa atgtggctat
    25261 ttcacggcga aaaaggagcg cgcaatgaag ttctggcgac ccggtattac cggcaaactg
    25321 tttctggcga ttttcgccac ctgcattgtc ttattgatca cgatgcactg ggcggtacgt
    25381 atcagttttg agcgcggctt tatcgattac atcaagcatg gtaatgaaca acggctgcaa
    25441 atgctcggcg atgcgcttgg tgagcagtac gcccagcacg ggaactggcg cttcctgcgt
    25501 aataacgatc gctttgtatt tcagatccta cgttcactgg agcatgataa caacgaagat
    25561 aagcccggcc ccggtatgcc gccacacggc tggcgcacac aattttgggt ggttgatcaa
    25621 aacaacaaag tgctggttgg cccgcgagca ccagttccac ccgacggcac acggcggccc
    25681 attatggtca atggtgcgga agttggcgcg gtgatcgcct cccctgttga acgactgacc
    25741 cgtaatactg atatcaattt tgacagacaa cagcggcaaa ccagttggct gattgtggct
    25801 ttatctacct tattagcggc gctggcgaca ttcccactgg cgcgcggttt gctggctccg
    25861 gtaaaacgac tggtggatgg cacgcacaaa ctggcagcgg gtgatttcac tactcgcgta
    25921 gcccccacca gcgaagatga gttgggcaga ctggcagagg atttcaatca actcgccagc
    25981 acactggaga aaaaccagca gatgcgccgc gattttatgg ccgatatttc tcatgaactg
    26041 cgcacgcctt tagcagtact gcgcggcgaa ctggaagcca ttcaggatgg tgtgcgtaaa
    26101 ttcacgccgg agacgctggc ttctttacag gcagaagtcg gtacactgac aaaactggtt
    26161 gacgatctcc atcaattgtc tatgtctgat gaaggcgctc tcgcctatca aaaagcaccg
    26221 atagatttga tcccactgct ggaagtggcg ggaggcgcat ttcgcgaacg attcgccagc
    26281 cgtggcctga aattgcaatt ttccctgcca gacagtatta ccgtatttgg cgatcgcgat
    26341 cgtttaatgc agttgttcaa taacttactg gaaaacagcc tgcgctacac tgacagcggc
    26401 ggcagcctga aaatctctgc cgagcagcac gacaaaacgg tgcgcctgac ctttgccgac
    26461 agtgcgccgg gtgtcagtga cgatcagcta caaaaattgt ttgaacgttt ttatcgcacc
    26521 gaaggttccc gcaaccgcgc cagcggtggt tccgggctgg ggctggcgat ttgcctgaac
    26581 attgttgaag cacataacgg ccgcattatt gccgcccatt cgccttttgg cgggcttagc
    26641 attacagtag agttaccgct ggaacgggat ttacagagag aagtatgacc gagttaccaa
    26701 tcgacgaaaa cacaccgcgt attttgatcg tggaagatga accgaagctg gggcagttgc
    26761 tcattgatta tctgcgtgct gcgagctatg cgccaacgtt gattagccac ggcgatcagg
    26821 tactgccata tgtgcgccag acaccaccgg atctgatcct gttggatctg atgctccctg
    26881 gcaccgatgg cctgacgctg tgccgggaaa ttcgtcgttt ttctgacatt ccgatcgtga
    26941 tggtgacggc aaaaatcgaa gagatcgacc gcctgctggg gttggagatt ggcgcagatg
    27001 actatatctg caagccgtac agcccacggg aagtggtagc gcgcgtcaaa accattttgc
    27061 gccgttgcaa accgcagcgt gagttgcagc aacaggatgc tgaaagcccg ttgattatcg
    27121 acgaaggtcg ttttcaggct tcctggcgcg gtaaaatgct tgacctgacg cctgcagaat
    27181 ttcgactgct gaaaacgctc tctcatgaac caggaaaagt gttctcccgc gagcagttgc
    27241 tgaaccatct ttatgacgac taccgcgtgg taaccgaccg taccatcgac agccacatta
    27301 aaaacctgcg ccgcaagctg gaatctctcg acgccgaaca gtcatttatc cgcgccgttt
    27361 acggcgtcgg ttaccgctgg gaagccgacg cctgccgcat cgtttaacat ttttacctgc
    27421 cggatctggc cttcagacca gatccggcaa cattatccca cgcatagtca gcaaactgac
    27481 atgatctctc cgccctggca ggcaccagaa aatcgctcat actttaatcg gcaaacagtc
    27541 cttttagatg ctgttttcga tacgcaattt caatcaagga gtcattatgg ctggttggtt
    27601 tgaactcagt aagagcagtg ataatcaatt ccggtttgtg ctaaaagcgg gcaatggtga
    27661 gactatcctc accagcgagc tgtatacctc aaaagcctct gcggaaaagg gcatcgcgtc
    27721 ggtacgtagc aacagcccgc aagaagaacg ctatgagaaa aaaacggcaa gcaacggcaa
    27781 attctatttc aatctgaaag ccgccaatca tcaaattatc ggctccagcc agatgtacgc
    27841 caccgcgcaa tctcgtgaaa ccggaattgc ctccgttaaa gcaaatggca caagccagac
    27901 ggtgaaagac aatacgtaat tacgatgccg ggcgtaggtt gttggcgtcc ggcgctttac
    27961 cagttacctc cacagcgcta caatgcccgc ccttaaagtg ggggcactcc cctgaccgct
    28021 tcatcaggtg aagcggatct gacctgtcat cagaacgaga gaattatgtt taaaccggaa
    28081 ctcctttccc cggcgggaac gctgaaaaat atgcgttacg ctttcgctta tggcgcagat
    28141 gctgtttatg cgggccagcc gcgttactcc ctgcgtgtgc gcaacaacga attcaaccac
    28201 gaaaatcttc aactcggcat taacgaagcc cacgcgctgg ggaaaaagtt ttatgtcgtg
    28261 gtcaacattg caccgcacaa cgccaagctg aaaaccttta tccgtgacct gaaaccggtg
    28321 gtggaaatgg ggccggatgc gctgattatg tccgatccag ggctgattat gctggtgcgt
    28381 gagcacttcc ctgaaatgcc gatccatctc tcggtacagg ctaacgccgt gaactgggcg
    28441 acggtgaaat tctggcagca aatgggcctg acccgcgtga tcctctctcg cgaactgtca
    28501 ctggaagaga ttgaagagat ccgcaatcag gtgccggata tggaaatcga aatcttcgtt
    28561 cacggcgcgc tgtgcatggc ctactccggt cgctgcctgc tctctggcta tatcaacaag
    28621 cgcgacccga accagggcac ctgcaccaac gcctgccgct gggagtacaa cgtccaggaa
    28681 gggaaagaag atgacgttgg caacatcgta cacaagtacg agccgattcc ggtgcaaaat
    28741 gttgagccga cgctgggtat cggcgcgcca accgacaaag tgtttatgat cgaagaagcc
    28801 cagcgtccgg gcgagtatat gaccgcgttt gaagatgagc acggcactta catcatgaac
    28861 tcgaaagatc tgcgcgccat cgcccatgtt gaacgcctga ccaaaatggg cgtgcattcg
    28921 ctgaaaatcg aaggccgtac caaatctttc tactattgcg cacgcaccgc gcaggtttac
    28981 cgtaaagcta tcgatgacgc cgctgcgggc aaaccgttcg ataccagcct gctggaaact
    29041 ctggaaggtc tggcgcatcg tggctatacc gaaggtttcc tgcgtcgtca tactcacgac
    29101 gattatcaga actacgaata cggttattca gtttctgacc gccagcagtt tgttggtgag
    29161 tttaccggtg agcgcaaggg ggacctcgcg gcggtagcgg tgaaaaataa attctccgtt
    29221 ggcgacagcc ttgagctaat gacgccgcaa ggcaacatta actttaccct tgagcacatg
    29281 gaaaacgcca aaggtgaagc tatgccggtc gcaccaggcg atggttatac tgtgtggctc
    29341 ccggtgccgc aggatcttga gctaaattac gcgctgctga tgcgtaattt ctccggggaa
    29401 accacgcgta acccccacgg taagtgatta atttcgatta tttttcccgg atggaaaatt
    29461 cttagaaacc gatcacatac agctgcattt attaaggtta tcatccgttt cgctgaaaaa
    29521 cataacccat aaaatgctag ctgtaccagg aaccacctcc ttagcctgtg taatctccct
    29581 tacacaggct tattttttat gcataagccc tatccctggt caccgtcttc cattgaccac
    29641 atcgatagaa tctcccttaa tagcacgatg cctttcactt atcgacatcg tgctcgcaca
    29701 ggttccggtt acgcacagcc agaacgcgca tgtttgacgc ttaccaaaaa atgaacctaa
    29761 agcattggaa tatttttgac atcattttct gatggctgca taaaataaaa attctgcttt
    29821 agtttcatct atctgtttgt cattattact cacattcaat aatggtgttg aagaatatcc
    29881 catcacagat aaaataaata tgtgcatggt agtcttgaat ctattctcac tctccacatt
    29941 tgaatgtcag acgagcaacg ccatgtaatc ctgcaccttc tgtcttcaat ttaactatct
    30001 gcattttttt gccctgagta acacagaaat gggctgcatc attttttact atattttctg
    30061 caccagatat tctgcccctg gctaaagaag cttcggcttc ggtgtagtat tggttatcga
    30121 gtttacgctg aatattactt ttatatgcaa gaccaaattt accgatactt gtctcatcat
    30181 tatgtacagc acacccagac ataagaaaaa tactaattaa tgatatagca gctatctttt
    30241 tcatctcacc ttc
//
LOCUS       DAHHTG010000054        28815 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.054, whole genome shotgun sequence.
ACCESSION   DAHHTG010000054 DAHHTG010000000
VERSION     DAHHTG010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28815)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28815)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28815
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.054"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            10..939
                     /gene="aaeR"
                     /locus_tag="NBI74_002306"
     CDS             10..939
                     /gene="aaeR"
                     /locus_tag="NBI74_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AaeR"
                     /protein_id="HCD1932630.1"
                     /translation="MERLKRMSVFAKVVEFGSFTAAARQLQMSVSSISQTVSKLEDEL
                     QVKLLNRSTRSIGLTEAGRIYYQGCRRMLHEVQDVHEQLYAFNNTPIGTLRIGCSSTM
                     AQNVLAGLTAKMLKEYPGLSVNLVTGIPAPDLIADGLDVVIRVGALQDSSLFSRRLGA
                     MPMVVCAAKSYLTQYGIPEKPADLSSHSWLEYSVRPDNEFELIAPEGISTRLIPQGRF
                     VTNDPMTLVRWLTAGAGIAYVPLMWVINEINRGELEILLPRYQSDPRPVYALYTEKDK
                     LPLKVQVVINSLTDYFVEVGKLFQEMHGRGKEK"
     gene            complement(1061..2506)
                     /gene="tldD"
                     /locus_tag="NBI74_002307"
     CDS             complement(1061..2506)
                     /gene="tldD"
                     /locus_tag="NBI74_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HCD1932631.1"
                     /translation="MSLNLVSEQLLAANGLKHQDLFAILGQLAERRLDYGDLYFQSSY
                     HESWVLEDRIIKDGSYNIDQGVGVRAISGEKTGFAYADQISLLALEQSAQAARTIVRD
                     SGDGKVQTLGAVEHSPLYTSVDPLQSMSREEKLDILRRVDKVAREADKRVQEVTASLS
                     GVYELILVAATDGTLAADVRPLVRLSVSVLVEEDGKRERGASGGGGRFGYEFFLADLD
                     GEVRADAWAKEAVRMALVNLSAVAAPAGTMPVVLGAGWPGVLLHEAVGHGLEGDFNRR
                     GTSVFSGQVGELVASELCTVVDDGTMVDRRGSVAIDDEGTPGQYNVLIENGILKGYMQ
                     DKLNARLMGMTPTGNGRRESYAHLPMPRMTNTYMLPGKSTPQEIIESVEYGIYAPNFG
                     GGQVDITSGKFVFSTSEAYLIENGKVTKPVKGATLIGSGIETMQQISMVGNDLKLDNG
                     VGVCGKEGQSLPVGVGQPTLKVNNLTVGGTA"
     gene            complement(2662..6462)
                     /gene="yhdP"
                     /locus_tag="NBI74_002308"
     CDS             complement(2662..6462)
                     /gene="yhdP"
                     /locus_tag="NBI74_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA2 domain-containing protein YhdP"
                     /protein_id="HCD1932632.1"
                     /translation="MRRLPGILLLTGAALVVIAALLVSGLRIALPHLDAWRPEILNKI
                     ESATGMPVEASQLSASWQNFGPTLEAHDIRAELKDGGEFSVKRVTLALDVWQSLLHMR
                     WQFRDLTFWQLRFRTNTSITSGGGNDSLEASHISDLFLRQFDHFDLRDSEVSFLTPSG
                     QRAELAIPQLTWLNDPRRHRAEGLVSLSSLTGQHGVMQVRMDLRDDEGLLSNGRVWLQ
                     ADDIDLKPWLGKWMQDNIALETAQFSLEGWMTIDKGDVTGGDVWLKQGGASWLGEKET
                     HTLSVDNLTAHITRENPGWQFSIPDTRITMDGKPWPSGALTLAWIPEQDVGGKDNKRS
                     DELRIRASNLELAGLEGVRPLVAKLSPALGDVWRSTQPSGKINTLALDIPLQAADKTR
                     FQASWSDLAWKQWKLLPGAEHFSGTLSGSVENGLLTASMKQAKMPYETVFRAPLEIAD
                     GQATISWLNNDKGFQLDGRNIDVKAKAVHARGGFRYLQPANDEPWLGILAGISTDDGS
                     QAWRYFPENLMGKDLVDYLSGAIQGGEADNATLVYGGNPQLFPYKHNEGQFEVLVPLR
                     NAKFAFQPDWPALTNLDIELDFINDGLWMKTDGVNLGGVRASNLTAVIPDYSKEKLLI
                     DADIKGPGKAVGPYFDETPLKDSLGATLQELQLDGDVNARLHLDIPLNGELVTAKGEV
                     TLRNNSLFIKPLDSTLKNLSGKFSFINGDLQSEPLTASWFNQPLNVDFSTKEGAKAYQ
                     VAVNLNGNWQPAKTGVLPAAVNEALSGSVAWDGKVGIELPYHAGATYNVELNGDLKNV
                     SSHLPSPLAKPAGEPLSVNVKVDGNLNSFELTGQAGADNYFNSRWLLGQKLTLDRAIW
                     AADSKTLPPLPEQSGVELNMPPMNGAEWLALFQKGAAESVGGAASFPQHITLRTPMLS
                     LGNQQWNNLSIVSQPTANGTLVEAQGREINATLAMRNNAPWLANIKYLYYNPSVAKTR
                     GDSTQSSPFPTTERISFRGWPDAQIRCAECWFWGQKFGRIDSDITISGDTLTLTNGLI
                     DTGFARLTADGEWVNNPGNERTSLKGKLRGQKIDAAAEFFGVTTPIRQSSFNVDYDLH
                     WRKAPWQPDEATLNGIIHTQLGKGEITEINTGHAGQLLRLLSVDALMRKLRFDFRDTF
                     GEGFYFDSIRSTAWIKDGVMHTDDTLVDGLEADIAMKGSVNLVRRDLNMEAVVAPEIS
                     ATVGVAAAFAVNPIVGAAVFAASKVLGPLWSKVSILRYHISGPLDDPQINEVLRQPRK
                     EKAQ"
     gene            complement(6530..7999)
                     /gene="rng"
                     /locus_tag="NBI74_002309"
     CDS             complement(6530..7999)
                     /gene="rng"
                     /locus_tag="NBI74_002309"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease G"
                     /protein_id="HCD1932633.1"
                     /translation="MTAELLVNVTPSETRVAYIDGGILQEIHIEREARRGIVGNIYKG
                     RVSRVLPGMQAAFVDIGLDKAAFLHASDIMPHTECVAGEEQKQFTVRDISELVRQGQD
                     LMVQVVKDPLGTKGARLTTDITLPSRYLVFMPGASHVGVSQRIESESERERLKKVVAE
                     YCDEQGGFIIRTAAEGVGEAELASDAAYLKRVWTKVMERKKRPQTRYQLYGELALAQR
                     VLRDFADAELDRIRVDSRLTYEALLEFTSEYIPEMTSKLEHYTGRQPIFDLFDVENEI
                     QRALERKVELKSGGYLIIDQTEAMTTVDINTGAFVGHRNLDDTIFNTNIEATQAIARQ
                     LRLRNLGGIIIIDFIDMNNEDHRRRVLHSLEQALSKDRVKTSVNGFSALGLVEMTRKR
                     TRESIEHVLCNECPTCHGRGTVKTVETVCYEIMREIVRVHHAYDSDRFLVYASPAVAE
                     ALKGEESHSLAEVEIFVGKQVKVQIEPLYNQEQFDVVMM"
     gene            complement(7989..8582)
                     /gene="yhdE"
                     /locus_tag="NBI74_002310"
     CDS             complement(7989..8582)
                     /gene="yhdE"
                     /locus_tag="NBI74_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase YhdE"
                     /protein_id="HCD1932634.1"
                     /translation="MTSLYLASGSPRRQELLAQLGVTFERIVTGIEEQRQPQESAQQY
                     VVRLAREKAQAGVAQTAQDLPVLGADTIVILNGEVLEKPRDAEHAAQMLRKLSGQTHQ
                     VMTAVALADSQHILDCLVVTDVTFRTLTDEDIAGYVASGEPLDKAGAYGIQGLGGCFV
                     RKINGSFHAVVGLPLVETYELLSNFNALREKRDKHDG"
     gene            complement(8591..9079)
                     /gene="mreD"
                     /locus_tag="NBI74_002311"
     CDS             complement(8591..9079)
                     /gene="mreD"
                     /locus_tag="NBI74_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001487551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HCD1932635.1"
                     /translation="MASYRSQGRWVIWLSFLIALLLQIMPWPDNLIVFRPNWVLLILL
                     YWILALPHRVNVGTGFVMGAILDLISGSTLGVRVLAMSIIAYLVALKYQLFRNLALWQ
                     QALVVMLLSLVVDIIVFWAEFLVINVSFRPEVFWSSVVNGVLWPWIFLLMRKVRQQFA
                     VQ"
     gene            complement(9079..10182)
                     /gene="mreC"
                     /locus_tag="NBI74_002312"
     CDS             complement(9079..10182)
                     /gene="mreC"
                     /locus_tag="NBI74_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HCD1932636.1"
                     /translation="MKPIFSRGPSLQIRLILAVLVALGIIIADSRLGTFSQIRTYMDT
                     AVSPFYFVSNAPRELLDGVSQTLASRDQLELENRALRQELLLKNSELLMLGQYKQENA
                     RLRELLGSPLRQDEQKMVTQVISTVNDPYSDQVVIDKGSVNGVYEGQPVISDKGVVGQ
                     VVAVAKLTSRVLLICDATHALPIQVLRNDIRVIAAGNGCTDDLQLEHLPANTDIRVGD
                     VLVTSGLGGRFPEGYPVAVVSSVKLDTQRAYTVIQARPTAGLQRLRYLLLLWGADRNG
                     ANPMTPEEVHRVANERLMQMMPQVLPSPDAMGPKLPEPATGITQPTPQQPATGNAVTA
                     PAAPTQPAANRSPQRATPPQSGAQPPARAPGGQ"
     gene            complement(10248..11291)
                     /gene="mreB"
                     /locus_tag="NBI74_002313"
     CDS             complement(10248..11291)
                     /gene="mreB"
                     /locus_tag="NBI74_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000913396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreB"
                     /protein_id="HCD1932637.1"
                     /translation="MLKKFRGMFSNDLSIDLGTANTLIYVKGQGIVLNEPSVVAIRQD
                     RAGSPKSVAAVGHDAKQMLGRTPGNIAAIRPMKDGVIADFFVTEKMLQHFIKQVHSNS
                     FMRPSPRVLVCVPVGATQVERRAIRESAQGAGAREVFLIEEPMAAAIGAGLPVSEATG
                     SMVVDIGGGTTEVAVISLNGVVYSSSVRIGGDRFDEAIINYVRRNYGSLIGEATAERI
                     KHEIGSAYPGDEVREIEVRGRNLAEGVPRGFTLNSNEILEALQEPLTGIVSAVMVALE
                     QCPPELASDISERGMVLTGGGALLRNLDRLLMEETGIPVVVAEDPLTCVARGGGKALE
                     MIDMHGGDLFSEE"
     gene            complement(11596..13536)
                     /gene="csrD"
                     /locus_tag="NBI74_002314"
     CDS             complement(11596..13536)
                     /gene="csrD"
                     /locus_tag="NBI74_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase E specificity factor CsrD"
                     /protein_id="HCD1932638.1"
                     /translation="MRLTTKFSAFVTLLTGLTIFVTLLGCSLSFYNAIQYKFSHRVQA
                     VATAIDTHLLSNDFSTLRPQITELMMSADIVRVDLLHGDKQVYTLARNGSYRPVGTND
                     LFRELSVPLIKHPGMSLRLVYQDPMGNYFHSLMTTAPLTGAIGFIILMLFLAVRWLQR
                     QLAGQELLETRATRILNGERGSNVLGTIYEWPPRTSSALDTLLCEIQNAREQHSRLDT
                     LIRSYAAQDMKTGLNNRLFFDNQLATLLEDQEKVGTHGIVMMIRLPDFNMLSDTWGHS
                     LVEEQFFSLTNLLSTFMMRYPGALLARYHRSDFAALLPHRTLKEAESIASQLIKAVDT
                     LPNNKMLDRDDMIHIGICAWRSGQDTEQVMEHAESATRNAGLQGGNSWAIYDDSLPEK
                     GRGNVRWRTLIEQMLSRGGPRLYQKPAVTREGRVHHRELMCRIFDGNEEVSSAEYMPM
                     VLQFGLSEEYDRLLISRLIPLLRYWPEENLAIQVTVESLIRPRFQRWLRDTLMQCEKS
                     QRRRIIIELAEADVGQHISRLQPVIRLVNALGVRVAVNQAGLTLVSTSWIKELNVELL
                     KLHPGLVRNIEKRTENQLLVQSLVEACSGTSTQVYATGVRSRSEWQTLIQRGVTGGQG
                     DFFASSQPLDTNVKKYSQRYSV"
     gene            13688..14662
                     /locus_tag="NBI74_002315"
     CDS             13688..14662
                     /locus_tag="NBI74_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HCD1932639.1"
                     /translation="MQALLLEQQDGKTLASVQTLDESRLPEGDVTVDVHWSSLNYKDA
                     LAITGKGKIIRNFPMIPGIDFAGTVRTSEDPRFHAGQEVLLTGWGVGENHWGGLAEQA
                     RVKGDWLVAMPQGLDARKAMIIGTAGFTAMLCVMALEDAGVRPQDGEIVVTGASGGVG
                     STAVALLHKLGYQVVAVSGRESTHEYLKSLGASRILPRDEFAESRPLEKQVWAGAIDT
                     VGDKVLAKVLAQMNYGGCVAACGLAGGFTLPTTVMPFILRNVRLQGVDSVMTPPARRA
                     QAWQRLVTDLPESFYTQAAKEISLAEAPKFAEAIINNQIQGRTLVKVN"
     gene            15639..16109
                     /gene="accB"
                     /locus_tag="NBI74_002316"
                     /gene_synonym="fabE"
     CDS             15639..16109
                     /gene="accB"
                     /locus_tag="NBI74_002316"
                     /gene_synonym="fabE"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HCD1932640.1"
                     /translation="MDIRKIKKLIELVEESGISELEISEGEESVRISRAAPAASFPVM
                     QQAYAAPMMQQPAQSNAAAPATVPSMEAPAAAEISGHIVRSPMVGTFYRTPSPDAKAF
                     IEVGQKVNVGDTLCIVEAMKMMNQIEADKSGTVKAILVESGQPVEFDEPLVVIE"
     gene            16120..17469
                     /gene="accC"
                     /locus_tag="NBI74_002317"
     CDS             16120..17469
                     /gene="accC"
                     /locus_tag="NBI74_002317"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009483995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HCD1932641.1"
                     /translation="MLDKIVIANRGEIALRILRACKELGIKTVAVHSSADRDLKHVLL
                     ADETVCIGPAPSVKSYLNIPAIISAAEITGAVAIHPGYGFLSENANFAEQVERSGFIF
                     IGPKAETIRLMGDKVSAIAAMKKAGVPCVPGSDGPLGDDMDKNRAIAKRIGYPVIIKA
                     SGGGGGRGMRVVRSDAELAQSISMTRAEAKAAFSNDMVYMEKYLENPRHVEIQVLADG
                     QGNAIYLAERDCSMQRRHQKVVEEAPAPGITPELRRYIGERCAKACVDIGYRGAGTFE
                     FLFENGEFYFIEMNTRIQVEHPVTEMITGVDLIKEQLRIAAGQPLSIKQEEVHVRGHA
                     VECRINAEDPNTFLPSPGKITRFHAPGGFGVRWESHIYAGYTVPPYYDSMIGKLICYG
                     ENRDVAIARMKNALQELIIDGIKTNVDLQIRIMNDENFQHGGTNIHYLEKKLGLQEK"
     gene            complement(17561..18607)
                     /locus_tag="NBI74_002318"
     CDS             complement(17561..18607)
                     /locus_tag="NBI74_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000787050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HCD1932642.1"
                     /translation="MKNEIWSWIGDLSQVAGIKHYELRSGRAKGTEAFDVRTGAGLAF
                     TVVKDRALDIAWASYKDTALSFITPNGVVAPAFFESQGNGFLRSFYAGLLTTCGLSYI
                     GTPCEDEGETLGLHGRLAATPAEEVGYRTERTDDSIEFVINGKVRETRLFGENLTLER
                     TIRCRYGENVMRIEDKVTNHGFTRQPLQILYHFNYGWPLLSPQAEILLSAKSITPRTP
                     HAAEGLTSHLEICTPQPGFDEQVYYLTLNSDSQGMSKVALVNAELGWGIYEKFDTMQL
                     PNFIQWKNLGAGEYVMGLEVSNSFPDGRDKERAQGRLPFIEPGETKKYCFELGIVDGD
                     AEISALKAEIAGYR"
     gene            complement(18604..19566)
                     /locus_tag="NBI74_002319"
     CDS             complement(18604..19566)
                     /locus_tag="NBI74_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000048618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HCD1932643.1"
                     /translation="MSKVFTIGEILVEIMASKIGQPFDQPGIWNGPYPSGAPAIFIDQ
                     VTRLGVPCGIISCVGNDGFGDINIHRLAADGVDIRGISVLPLEATGSAFVTYHNSGDR
                     DFIFNIKNAACGKLSAQHVDENILKDCTHFHIMGSSLFSFHMVDAVKKAVTIVKANGG
                     VISFDPNIRKEMLDIPEMRDALHFVLELTDIYMPSEGEVLLLSPHSTPERAIAGFLEE
                     GVKEVIVKRGNQGASYYSANEQFHVESYPVEEVDPTGAGDCFGGAWIACRQLGFDAHR
                     ALQYANACGALAVTRRGPMEGTSRLMEIETFIQRHDMSIREAAQ"
     gene            complement(19588..20577)
                     /locus_tag="NBI74_002320"
     CDS             complement(19588..20577)
                     /locus_tag="NBI74_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001525780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCD1932644.1"
                     /translation="MTEKTLASPKPSGIPIANNFKKVFRQYGGILSGMVVLIILFSFI
                     NDSFFTANNITNIILQVSIIAITAYGMTYVLLLGDIDLSVGSTIALIGTFAALGASWG
                     IPFVLLVPLSIIAALALGMINGGLTAIAGIPSFIVTVATMGIFRGIAYIVTDGMPIMI
                     KDDAFLALGNGEFLYIPIPIWILIILLLINHFILTKTTFGRKIYITGGNKEAAIYSGI
                     NVTRLKIKVFMITAVLAGISGMILASRLYSGQPNSALSYELDAIAAAVLGGTSLNGGY
                     GTVVGTVIGALTIGVINNGMNLMNVPYFYQMVVKGLVILVAVYFDVRNKRKRS"
     gene            complement(20578..22077)
                     /locus_tag="NBI74_002321"
     CDS             complement(20578..22077)
                     /locus_tag="NBI74_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020245594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HCD1932645.1"
                     /translation="MTDVILDVSHIAKTFGHVQALKDITLSLRKGRVHTLLGENGAGK
                     STLMKILAGVYPPTQGTITLRGETITINNPQHSRQLGIAIIFQELSLSNNMTVAENIY
                     ANNEPRRFGIINDKKMLADCQNLLAELGIPLDPLEMVGNMSMAHRQLVEIAKALSYAA
                     DVVIMDEPTSSLSDNEAEILFNIIEKLKQRGCAVIYISHRMDEIMRISDDISVIRDGE
                     YIATHEKKNSDIQHLIAQMVGREMKNIWPARLGEKPDENVPAKLEVKNLSHPSLFKEV
                     SFAVRPGEVLGFFGLVGAGRSDVMKALFGLVSYHGTVLIDGKEVRIANPKQAIDHGIA
                     FVTENRKEEGLVLMHDVNMNTHHVAFQYNASRMGLINHRQEEAKTLQSIARMNTKVSS
                     VHQAVGALSGGNQQKIVLSKWLEKTPRILLLDEPTRGVDVGAKFEIYNVIRQLAAAGT
                     AIILVSSELPEVMALSDRLVVMRNKTIADIYSCENLTQTQVMTAATGVR"
     gene            complement(22138..23028)
                     /locus_tag="NBI74_002322"
     CDS             complement(22138..23028)
                     /locus_tag="NBI74_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001341904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HCD1932646.1"
                     /translation="MKNIKLLGSAMLVSLALFSQSSLAKEYKIGASLLTQQHPFYIDL
                     ANAMKEEAKKDNVNLSVSIANQDLNKQLSDVEDFITKKVDAIIISPVDSKGVQAAIIK
                     ADKAGIPVITVDVAAEGVPVVSHVATDNYAGGVEAGKLMGKLLSGKGTVAIISYPALQ
                     SVVARVDGFKKGLSDTPDIKIVAEQPGITRAEALTTAQNIMQANANLNGLFGFGDDAA
                     LAAVIAAKSAHNDNIKIIGFDGMKEARDAVDSEKTFAAVIRQYPDQMGAKAIDAAVDH
                     LNGKPVEKMIPVQPGVYTGK"
     gene            complement(23064..23918)
                     /locus_tag="NBI74_002323"
     CDS             complement(23064..23918)
                     /locus_tag="NBI74_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000275530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose bisphosphate family class II aldolase"
                     /protein_id="HCD1932647.1"
                     /translation="MYIISSKNMLKKAQSEGYAVPAFNIHNLETLQVVVESAAQLRSP
                     VMLAGTPGTYRYGGVGSLISLVQSLAREYNLPLVLHLDHHEESDDIFNKVRAGIRSVM
                     IDGSHLPFEDNIARVQEVTTFCHRFDVSVEAELGRLGGQEDDLRVDEKDSAYTSPAAA
                     TEFVQRTQIDSLAVAIGTAHGLYAHEPRLDFVRLEQIRQRVEIPLVLHGASGLSEQDV
                     QHCIRRGICKVNVATELKIAFADALKGYFYNNPAANDPRHYMQPAKAAMKEVVTRIIG
                     VCGSEGKI"
     gene            24259..25089
                     /locus_tag="NBI74_002324"
     CDS             24259..25089
                     /locus_tag="NBI74_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000843961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCD1932648.1"
                     /translation="MKSVNVPRKSSNLLKRRLKIAEIVAAQGEIKVDDLSAQLGVSGV
                     TIRGDLSYLEQQGYLKRSFGGAIATPLSSEPEAIQPIAPLSLAQQMEIARHCARLIRD
                     RDTLFLGHGTICRKIIPLLSEVKKLRLITNDPEHALLANQFIDGEIILAGSEMLRPDT
                     ALAGKQLEQALQHFTITHSILEISHLDADGTLNINVPRLAAAWQLCFDHAQNKTVIVA
                     ADPAPSTAAIGHLHQAENVIVSRSINEQYQQQLQSADFVILYTSNECFTWSNRERLQE
                     "
     gene            25121..26056
                     /locus_tag="NBI74_002325"
     CDS             25121..26056
                     /locus_tag="NBI74_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001446002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HCD1932649.1"
                     /translation="MGELLAEFLSRNPHQKFTQPGEFIGPFPSGAPAIFAAQVAKLSH
                     RAIFFGCVGNDDFARLIIERLRHEGVITDGIHVMNNAVTGTAFVSYQNPQLRDFVFNI
                     PNSACGLFTAEHIDKDLLKQCNHLHIVGSSLFSFRMIDVMRKAITTIKSAGGTVSFDP
                     NIRKEMLSIPEMAQALDYLIEYTDIFIPSESELPFFARHKNLSEEQIVSDLLHGGVKH
                     VAIKRAQRGASYYKLKNGTLHAQHVAGHDIEIIDPTGAGDCFGATFITLFLSGFPAHK
                     ALQYANASGALAVMRQGPMEGISSLADIEDFLQQH"
     gene            26149..26391
                     /locus_tag="NBI74_002326"
     CDS             26149..26391
                     /locus_tag="NBI74_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhdT family protein"
                     /protein_id="HCD1932650.1"
                     /translation="MDTRFVQAHKEARWALGLTLLYLAVWLVAAYLPGVAPGFTGFPR
                     WFEMACILTPLLFIGLCWAMVRFIYRDIPLEDDDAA"
     gene            26381..27832
                     /gene="panF"
                     /locus_tag="NBI74_002327"
     CDS             26381..27832
                     /gene="panF"
                     /locus_tag="NBI74_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/pantothenate symporter"
                     /protein_id="HCD1932651.1"
                     /translation="MQLEVILPLVAYLVVVFGISVYAMRKRSTGTFLNEYFLGSRSMG
                     GIVLAMTLTATYISASSFIGGPGAAYKYGLGWVLLAMIQLPAVWLSLGILGKKFAILA
                     RRYNAVTLNDMLFARYQSRLLVWLASLSLLVAFVGAMTVQFIGGARLLETAAGIPYET
                     GLLIFGISIALYTAFGGFRASVLNDTMQGLVMLIGTVVLLIGVVHAAGGLSNAVDTLQ
                     TIDPQLVTPQGADDILSPAFMTSFWVLVCFGVIGLPHTAVRCISYKDSKAVHRGIIIG
                     TIVVAILMFGMHLAGALGRAVIPDLTVPDLVIPTLMVKVLPPFAAGIFLAAPMAAIMS
                     TINAQLLQSSATIIKDLYLNIRPDQMQNETRLKRMSAVITLVLGALLLLAAWKPPEMI
                     IWLNLLAFGGLEAVFLWPLVLGLYWERANAKGALSAMIVGGVLYAVLAKLNIQYLGFH
                     PIVPSLLLSLLAFLVGNRFGTSVPQATVLTTDK"
     gene            27844..28725
                     /gene="prmA"
                     /locus_tag="NBI74_002328"
     CDS             27844..28725
                     /gene="prmA"
                     /locus_tag="NBI74_002328"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HCD1932652.1"
                     /translation="MPWIQLKLNTTGANAEDLSDALMEAGAVSITFQDTHDTPVFEPL
                     PGETRLWGDTDVIGLFDAETDMNDVVAILENHPLLGAGFAHKIEQLEDKDWEREWMDN
                     FHPMRFGERLWICPSWRDVPDENAVNVMLDPGLAFGTGTHPTTSLCLQWLDSLDLTGK
                     TVIDFGCGSGILAIAALKLGAAKAIGIDIDPQAIQASRDNAERNGVSDRLELYLPKDQ
                     PEEMKADVVVANILAGPLRELAPLISVLPVSGGLLGLSGILASQAESVCEAYADSFAL
                     DPVVEKEEWCRITGRKN"
BASE COUNT         7079 a         7654 c         6968 g         7114 t
ORIGIN      
        1 gagtgaataa tggaacgact aaaacgcatg tcggtgtttg ccaaagtagt tgaatttggc
       61 tcttttaccg ccgccgccag acagctacag atgagcgttt cgtccatcag tcagacggtg
      121 tcaaaactgg aagatgagtt gcaggtaaag ctgttaaacc gtagcacacg cagcattggc
      181 ctgaccgaag ccggtagaat ttactaccag ggctgccgtc ggatgctcca tgaagtgcag
      241 gatgttcatg agcaactgta tgccttcaat aacaccccca tcgggacgct gcgcattggc
      301 tgttcttcaa ctatggcaca aaatgttctc gccgggctga cagcaaaaat gctgaaagaa
      361 tacccaggtt tgagcgtcaa tctggttacc ggaattcctg cccccgacct gattgccgac
      421 ggtctggatg tggtgatccg cgtcggcgcg ttgcaagatt ccagcctgtt ttcccgccgt
      481 ctgggcgcga tgccaatggt ggtgtgcgcc gcaaaaagct atctcacaca atacggcata
      541 ccggaaaaac ccgccgattt aagtagtcac tcatggcttg aatacagcgt gcggcccgac
      601 aatgaatttg aactgatcgc accggaaggg atctcgactc gcctgatccc acaaggaaga
      661 tttgtgacta atgatccgat gacgctggtg cgctggctga cggcgggtgc cgggatcgcc
      721 tacgtgccgc tgatgtgggt gatcaacgag atcaatcgtg gggagctgga gatcctgctg
      781 ccacgttacc agtcagatcc acgcccggtt tatgcgttat ataccgaaaa agataagctg
      841 ccgctgaagg tacaggtcgt gatcaactcg ctgacggatt attttgttga ggtcggtaaa
      901 ttgtttcagg agatgcacgg gcgcgggaaa gagaagtaat taatattgta ctgttacgtt
      961 gtacaaacct gtgccgacgg gttcccctaa ccccagaggg gcgaggggac cgttcgtgca
     1021 cggagaagga ttaattatcc ttctgaaaat actgaaatta ttacgcagta ccgccaacag
     1081 tcaggttatt aactttcagc gttggctggc ccacgccaac cggcaaactt tgcccttctt
     1141 taccgcagac acccacgccg ttatccagtt tcaggtcgtt gccaaccatc gaaatctgct
     1201 gcatggtttc gataccggaa ccaatcaacg ttgcgccttt caccggctta gttactttac
     1261 cgttttcaat cagatacgct tctgaggtgg agaaaacgaa tttgccggag gtgatatcca
     1321 cctgaccgcc accaaagttc ggtgcataga taccgtactc aacggattca ataatttcct
     1381 gcggggtcga tttacccggc agcatatagg tgttggtcat acgcggcatg ggcagatggg
     1441 cgtaggattc acggcgaccg ttgccagtcg gcgtcatccc catcaaacgc gcgttgagtt
     1501 tatcctgcat gtagcctttc agaatgccgt tctcaatcag cacgttgtac tggcctggcg
     1561 taccttcgtc atcaatcgcc accgaaccac ggcgatcgac catcgtgcca tcatcaacca
     1621 cggtacacag ttctgaagcc accaactccc cgacctgtcc actaaatact gaagtgccac
     1681 ggcggttgaa gtcgccttcc agaccgtgac ccaccgcttc atgcaacagc acgcccggcc
     1741 aacctgcgcc aagtactacc ggcatggtgc ccgctggtgc agcaacggca gaaagattga
     1801 ccagcgccat acgcactgct tcttttgccc acgcatccgc acggacttcg ccgtcgagat
     1861 cggcaaggaa gaactcataa ccaaaacgac cgccgccacc actggcaccg cgttcgcgtt
     1921 tgccatcttc ttcgaccaaa acgctcacgg aaagacgcac cagcggacgg acatccgccg
     1981 ctagtgtgcc gtcggtggcc gcaaccagaa ttaattcata gacaccactg aggctggcag
     2041 tcacttcctg tacgcgcttg tccgcttcgc gggcaacctt atcgacgcga cgcaggatat
     2101 ccaacttctc ttcacggctc atgctttgca gcggatctac cgaggtatat aacgggctat
     2161 gctctaccgc gcccagcgtc tgtactttgc catcaccact atcacggacg atggtgcgcg
     2221 ccgcttgtgc actctgttcc agcgccagca ggctgatttg gtcagcataa gcaaatccgg
     2281 ttttttcacc actgattgca cgcacaccaa cgccctgatc gatgttgtaa gaaccatctt
     2341 taataatacg gtcttctaaa acccaggatt cgtgatagct cgactgaaaa tagagatcgc
     2401 cataatcaag gcgacgttcg gccagttgac cgaggatcgc gaacaagtcc tgatgtttca
     2461 ggccgttcgc cgctagcaat tgttcactta ccaggttcag actcatcgtt tttgctactc
     2521 gttagttact gcagtagagg attttttacg gctgccggat gcggcgtgaa cgccctgtcc
     2581 ggcctacggt tatgttctgg ctcgttggcc tttggcaacg attatcatat gagattgggg
     2641 caattacgcg ccctcgtcaa atcattgcgc tttttcttta cgcggttgtc gcaacacttc
     2701 gttgatttgc ggatcgtcca gcggacccga aatgtgatag cgcaaaatgg agactttgct
     2761 ccacagcggt cccagcactt tactggcggc aaacactgcc gcgccaacaa tggggttaac
     2821 cgcaaaggcc gcagccacac ccaccgtcgc agaaatctct ggtgcgacaa ccgcttccat
     2881 attcaggtcg cgacgtacca gatttaccga ccctttcatg gcgatgtccg cttccaggcc
     2941 atccaccagc gtgtcgtcgg tgtgcataac gccgtcttta atccacgcgg tgctgcgaat
     3001 ggagtcaaaa tagaaccctt cgccaaaagt gtctctgaaa tcaaaacgca gcttacgcat
     3061 cagggcatct acgctcaata agcgcagcaa ttgcccggca tgtccggtat tgatttcggt
     3121 aatttcgcct ttacccagtt gagtatgaat gatgccattc aacgtcgcct catctggctg
     3181 ccagggtgct ttgcgccagt gtaaatcgta atccacatta aatgacgact ggcgtatggg
     3241 cgtcgtgaca ccaaaaaatt ctgcggcggc atcaattttc tgcccgcgca gttttccttt
     3301 cagcgaggtt cgttcattcc ccggattgtt aacccattca ccatcggcag tgagccgcgc
     3361 aaaaccagta tcaatcagtc cattagtcag cgttaacgta tcgccagaaa tggtgatatc
     3421 actgtcaatg cgaccgaatt tttgccccca gaaccagcac tctgcgcatc gtatttgtgc
     3481 gtctggccag ccacggaagc taatgcgttc cgttgtcggg aaaggtgatg actgtgttga
     3541 atcaccacga gttttcgcca cgcttgggtt gtaataaaga tatttgatat tcgccagcca
     3601 cggcgcgtta ttacgcatcg ccagcgtggc gttgatttca cgcccttgcg cctcaaccag
     3661 ggtgccattt gccgtcggtt gcgaaacaat actcaggtta ttccattgct gatttcccag
     3721 cgacaacata ggcgtacgta acgttatgtg ttgtgggaaa ctcgctgcac caccgacact
     3781 ctccgccgcg cctttctgga acagggccag ccactcggca ccattcatcg gcggcatatt
     3841 gagttcaacg ccactttgtt ccggcaacgg cgggagcgtt ttactgtctg ccgcccaaat
     3901 agcacgatcg agcgtcagct tttgaccgag caaccagcgg ctattgaaat agttatccgc
     3961 accagcctgt ccggttaatt caaagctgtt gagattgcca tcaaccttaa cgtttaccga
     4021 tagtggttca cccgcaggtt tggctaacgg tgaaggtaag tgactgctca cattcttcaa
     4081 atcgccgttc agctctacgt tatacgtcgc gccagcatga taaggcagct caatgcccac
     4141 tttaccatcc cacgccacgc tgccacttaa tgcttcgttc accgctgcag gcagaacgcc
     4201 ggttttcgct ggttgccagt taccgttgag attcaccgcg acctggtagg cttttgcccc
     4261 ttctttggtg gaaaaatcca cgttcaacgg ctgattaaac cagcttgctg tcagtggttc
     4321 actttgcaga tcgccattga taaagctgaa tttaccgctc aaatttttca gggtgctgtc
     4381 gagtggtttg ataaacagac tgttattacg cagcgtcact tcacctttcg cggttaccaa
     4441 ctcgccgttc agcgggatat caagatgtaa gcgagcattc acatcgccgt cgagctggag
     4501 ttcttgcagg gtcgcaccca gggaatcttt cagcggtgtc tcatcaaagt aagggccaac
     4561 ggctttaccc ggacctttaa tgtcagcgtc aatcagcagt ttttcttttg agtagtcagg
     4621 gatcactgcg gtgagattac tcgcgcgcac gccgcccaga ttaacgccat cggttttcat
     4681 ccataaaccg tcgttaataa agtccagttc aatatcaagg ttagttaatg caggccagtc
     4741 cggctggaag gcaaacttcg cgttgcgcag cggcaccagc acttcaaact gaccttcgtt
     4801 gtgtttatag gggaagagtt gcggattgcc accataaacc agcgtcgcgt tatccgcttc
     4861 accgccctga atcgcgccac ttaagtaatc aaccaggtct ttacccatca agttttccgg
     4921 gaaatagcgc caggcttgtg aaccatcatc ggtactgatg ccagccagaa tacccagcca
     4981 gggttcatcg ttagcaggtt gcaggtaacg aaaaccgccg cgcgcatgga cggctttggc
     5041 tttaacgtca atattacgcc catccagctg gaaacctttg tcattgttca gccagcttat
     5101 cgttgcctgg ccgtcggcga tttccagtgg cgcacggaat accgtttcgt aaggcatctt
     5161 tgcctgcttc atcgacgcag taagcaaacc attttctacg ctgccggaaa gcgtcccgga
     5221 gaagtgttcc gcacccggta ataatttcca ttgcttccag gccagatcgc tccacgatgc
     5281 ctgaaaacgg gtcttgtctg ccgcctgaag cgggatatcc agcgccagag tgttaatctt
     5341 gccgctcggt tgtgtggagc gccaaacatc acccagtgca ggtgaaagtt tcgcgaccag
     5401 cgggcgtacg ccctccagcc ctgccagctc cagattactg gcacgaatcc ggagttcgtc
     5461 actgcgttta ttgtctttgc cgccaacgtc ctgttccggt atccaggcca gcgtcaatgc
     5521 tccgctcggc cagggtttgc cgtccatcgt gatccgtgta tcgggaatag agaactgcca
     5581 tcccggattt tcacgcgtaa tatgcgcagt cagattatcc accgacagcg tatgcgtttc
     5641 cttctcgccc aaccagctgg caccgccctg tttcagccag acgtcaccgc cggttacatc
     5701 gcctttgtcg atcgtcatcc agccttcaag ggagaactgt gccgtttcca gcgcaatatt
     5761 gtcctgcatc catttaccga gccacggctt caggtcgatg tcatccgcct ggagccagac
     5821 gcgaccattg cttaacaacc cctcatcatc gcgcaaatcc atacgcacct gcatcacgcc
     5881 gtgctgtccg gtaaggctgg agaggcttac caggccttcc gcacggtgtc gacgtggatc
     5941 gttcagccag gtgagctgtg ggatcgccag ctcggcgcgc tgaccggatg gcgtcaggaa
     6001 actgacttca ctgtcgcgga gatcgaaatg gtcaaattga cgaagaaaca gatcgctgat
     6061 gtgactggct tccaggctat cattaccacc gccgctggtg atagaagtgt tggtgcgaaa
     6121 gcgcagctgc cagaaagtga ggtcgcgaaa ctgccagcgc atatgtaaca ggctctgcca
     6181 gacatccagc gccagggtaa cgcgtttaac cgaaaattcg ccgccatctt ttagttctgc
     6241 acggatgtcg tgcgcttcaa gcgtcggacc aaaattctgc cagctggctg agagctgact
     6301 ggcttctacc ggcatgccag tcgcggattc tattttgttg aggatttccg gacgccaggc
     6361 gtcaagatgc ggtaaagcaa tacgcaggcc gctaaccagc agggcagcga tcacaacgag
     6421 cgcggctcca gtaagcagta aaatccccgg caatcgcctc acccgtcact ccttgtctgc
     6481 taaaaatgtg actcaaaaac cctttgccgg atggcggccc agcatctgtt tacatcatta
     6541 cgacgtcaaa ctgctcctgg ttatagagcg gttcaatttg tactttaacc tgtttgccaa
     6601 cgaaaatttc cacttccgcc agcgagtgtg actcttcgcc tttcaaggct tcggctactg
     6661 ccggagaagc atagaccagg aaacggtcgg agtcgtaagc atggtggaca cgaacaatct
     6721 cgcgcatgat ttcatagcat accgtttcca cggttttcac cgttccgcga ccgtggcagg
     6781 ttgggcattc gttacacagt acgtgctcaa tgctttcgcg ggtgcgttta cgcgtcatct
     6841 ccaccagccc cagcgccgaa aaaccattaa cgctggtttt cacccgatct ttgctcaacg
     6901 cctgctccag cgagtgcagc acgcggcgac ggtgatcttc attattcata tcgatgaaat
     6961 caataatgat aatcccgccc agattacgca accgtaactg gcgagcgata gcctgcgtcg
     7021 cttcaatatt ggtattgaaa atggtgtcgt ccagattgcg atgaccgaca aacgccccgg
     7081 tattgatgtc cacggtggtc atcgcttcgg tctggtcgat aatcagataa ccaccggatt
     7141 tcagttctac tttgcgttcc agcgctcgct ggatttcgtt ttcgacatca aagagatcga
     7201 aaatcggctg gcgtccggtg taatgctcca gcttgctggt catctcggga atgtactctg
     7261 aggtaaactc cagcaatgct tcgtaagtca ggcgtgagtc aacgcgaatg cggtcgagtt
     7321 cggcgtcggc gaaatcacgc agaacacgct gcgccagcgc cagttcgccg tacagctgat
     7381 aacgggtctg cgggcgtttt ttacgctcca taactttggt ccagacgcgt ttcagataag
     7441 cagcatcgga ggccagttcg gcctcgccaa ccccttccgc tgcagtacgg atgataaacc
     7501 cgccctgctc gtcgcaatac tctgcgacca cttttttcag gcgttcacgt tctgattcgc
     7561 tttcaattcg ttgggatacc ccaacgtgag aagcccctgg cataaacacc agatagcgag
     7621 aaggcagcgt gatatcggtg gtcaggcgcg cacccttagt gccaagcgga tctttcacca
     7681 cctgcaccat cagatcttgc ccctgacgga ccagttccga gatgtcgcgc accgtgaatt
     7741 gcttttgttc ttcacccgcc acacattcgg tgtgcggcat gatgtcagat gcatgaagaa
     7801 acgcggcttt atccagccca atatctacaa aagccgcctg catacccgga agtacacgac
     7861 ttacacgacc cttgtagata ttgcctacta ttccgcgtcg cgcctcacgt tcaatatgaa
     7921 tttcctgcag aataccgcca tcaatatacg ccactcgagt ttccgaaggc gttacgttta
     7981 ccaacaattc agccgtcatg tttatccctt ttctcacgca gtgcgttaaa attacttaat
     8041 aattcatacg tttcaaccag cggtaagccg actacggcgt gaaagctgcc atttatcttc
     8101 ctgacaaaac agccacccag cccctgaata ccgtatgcac ctgctttatc taacggttcg
     8161 ccgctggcga catagcccgc gatgtcttcg tctgttaacg ttctgaaagt cacatcggtg
     8221 accaccaggc aatcgagaat gtgctggctg tcggccaacg ccactgccgt catcacctga
     8281 tgggtctgac ccgataattt gcgcaacatc tgcgccgcat gctctgcgtc gcgcggtttc
     8341 tcgagcactt ctccattcag gataacgata gtatccgcac ccagcaccgg gagatcctgc
     8401 gccgtttgcg cgacacccgc ctgtgctttc tcgcgcgcca gacgcacaac atactgctgc
     8461 gcgctctcct gcggctgacg ctgctcctca atgcccgtaa caatacgttc aaaggtcacg
     8521 ccaagttgcg caagtaactc ctgacgacgc ggagaaccgg aagctaaata cagagaagtc
     8581 atagaaacct ttattgcact gcaaactgct gacggacttt acgcatcagc aagaaaatcc
     8641 acggccagag aacgccattg actacactac tccagaacac ttccggtctg aaagagacgt
     8701 taatcactaa aaactctgcc cagaaaacaa taatatccac caccagcgaa agcaacatga
     8761 cgaccagcgc ctgctgccat aatgcgaggt tgcggaaaag ctggtatttc agcgccacca
     8821 ggtaagcaat gatgctcatc gccaatacgc gtacgccaag cgtcgagccg ctgatcagat
     8881 ccagtatggc acccatcaca aaacctgtgc ccacatttac gcgatggggc aaggccagga
     8941 tccaatacaa caagatgagt aacacccagt ttggccggaa aacaatcagg ttgtccggcc
     9001 atggcatgat ttgcagcaac agcgcaatga ggaaagagag ccagattacc cagcgtccct
     9061 ggctacgata gctcgccact attgtcctcc cggcgcacgc gcaggcggtt gagcaccact
     9121 ttgcggcggc gtagcccttt gtggagagcg attagcagcg ggctgtgtgg gcgcagcagg
     9181 cgcagtaact gcatttcctg tcgccggttg ctgcggagtc ggctgagtga tccccgttgc
     9241 cggttcaggt aactttggcc ccatcgcgtc tggcgaaggc aatacctgcg gcatcatctg
     9301 catcagacgt tcattagcaa cacgatgcac ctcttccggc gtcatcgggt tagcgccgtt
     9361 acgatccgcg ccccacagca gcagcagata acgcaaacgt tgcagtcctg cagtcggacg
     9421 cgcctgaatc acagtataag cgcgctgggt atcgagtttt acggaagaga caaccgcgac
     9481 cggatagcct tccgggaaac gaccgcccag accggaagtc accagcacat caccaacacg
     9541 aatatccgta ttcgctggca gatgctcaag ctgcaaatca tccgtacaac cgttaccggc
     9601 tgcaattacg cggatatcgt tgcgcagcac ctggattggc agcgcgtggg tcgcatcaca
     9661 aatcagcagc acgcggctgg tcagtttagc gacggccacc acctgaccaa caacaccttt
     9721 gtcgctgata accggctggc cttcataaac gccattaacg ctacctttat cgataacaac
     9781 ttgatcgcta taaggatcgt taaccgtgga gataacctga gtcaccattt tctgctcatc
     9841 ctgacgcagc ggggaaccca gcagctcgcg cagacgcgcg ttctcctgtt tgtattgtcc
     9901 aagcatcagc agttcactgt ttttcagcaa cagttcctga cgtaacgccc ggttttcaag
     9961 ttctagttgg tcacgcgagg ccagcgtctg cgatacgcca tccagcaatt cacgaggagc
    10021 attggaaaca aagtagaaag gactgacggc ggtatccata taagtacgga tttgactgaa
    10081 cgtccccagg cggctgtcgg caataataat gccgagcgcc accagcaccg ccagaataag
    10141 gcgaatctgt agcgacgggc cacggctaaa aattggcttc ataagttatg cgtattctcg
    10201 tatcagacca ggcagggtaa acagacactt cccctgcctg catccgatta ctcttcgctg
    10261 aacaggtcgc cgccgtgcat gtcgatcatt tccagcgctt tgccgccacc gcgcgccaca
    10321 caggtcagcg ggtcttcagc aacaacgact ggaatgccgg tttcttccat taacaaacgg
    10381 tcaaggttac gcagcagtgc gccaccaccg gtgagcacca tgccgcgctc ggagatgtcg
    10441 gaagccagtt ccggcgggca ctgttccagt gcaaccatta ccgcgctcac aataccggtc
    10501 agcggttcct gcagtgcttc gaggatttcg ttggagttca gggtaaaacc gcgtggaaca
    10561 ccttctgcaa ggttacggcc acgaacttcg atttcacgga cttcatcgcc cgggtaagcc
    10621 gaaccgattt cgtgcttgat acgttctgcg gtggcttcac cgatcagaga accgtaatta
    10681 cgacgtacat agttgatgat agcttcgtcg aaacggtcac caccaatgcg cacggaagag
    10741 gagtaaacca cgccgttcaa ggagataaca gcaacttcag tagtaccacc gccgatatca
    10801 accaccatag aaccggtcgc ttcagaaacc ggcaggccag caccaattgc ggcagccatc
    10861 ggttcttcaa tcaggaagac ttcacgggca ccagcgccct gcgcggattc acgaattgcg
    10921 cggcgttcaa cctgggtcgc gccaaccggc acacaaacca gaacgcgcgg gcttggacgc
    10981 ataaagctgt tgctgtgcac ttgtttgatg aagtgctgga gcattttttc agtcacgaag
    11041 aagtcggcga taacgccgtc tttcattggg cgaatggcag caatattgcc cggcgtacgg
    11101 cccagcatct gcttcgcgtc atgacctact gcagctacgc ttttcggtga accggcacga
    11161 tcctgacgaa tggccaccac ggaaggctca ttcaatacga tgccttgtcc ttttacataa
    11221 atgagggtat tcgcagtacc caggtcaatg gacaagtcat tggaaaacat gccacgaaat
    11281 tttttcaaca tactaaggga taatcctgaa agctggggcg gaaaagaaaa tccgcttact
    11341 ttaccaacca cacgcagcag cgacaaggcg caaaaatcat ctgctacggt gaaaattagt
    11401 gcagttcgtt tcctttgtta caaatctctg cctgagtcca gaaaggctta atgcatcagc
    11461 agcattcctc gcctgtttgc aaccgcgtaa ggtcattcat ctgcatatgt gctgcaacaa
    11521 tctggcgagc agacaagcac actcccatga gacgcagcgc gcgttattct acgtgaaaac
    11581 ggattaaacg gcaggttaaa ccgagtatct ttgtgaatat tttttcacgt tagtatcaag
    11641 tggctgtgag gacgcgaaaa aatccccttg cccacctgta acaccgcgct gaatcagggt
    11701 ctgccactcg cttcgcgaac gcacgccggt ggcgtaaacc tgggtgctgg tcccggagca
    11761 ggcttccacc aggctttgaa ccagcagctg gttctccgtt cgcttctcaa tgtttctgac
    11821 cagccccgga tggagcttga gtaactcaac attaagttct ttgatccaac tggtgcttac
    11881 cagcgtcaaa ccagcctggt tgacggctac ccgtacccct aaagcattca ctaaacgaat
    11941 aacaggttgt aagcgactga tatgttgacc aacatcggcc tctgcaagtt caataattat
    12001 gcgtcttcgt tgcgattttt cacattgcat taacgtatcg cgcagccaac gctgaaaacg
    12061 cgggcgaatc agcgactcaa cggtaacctg aatcgccaga ttttcctctg gccagtaacg
    12121 caatagtgga ataagacggc tgattagcaa gcggtcatac tcttccgata aaccaaactg
    12181 caagaccatc ggcatatact ctgccgagct aacctcttca ttaccatcga agatgcggca
    12241 catgagttca cgatgatgaa cccggccttc gcgagtaacc gccggttttt gataaaggcg
    12301 cgggccgccg cgactgagca tttgctcgat aagcgtacgc cagcgaacat taccgcgtcc
    12361 tttttcaggc aacgagtcat cgtaaatagc ccagctattg ccgccctgca atcccgcatt
    12421 acgcgtggca gactctgcat gttccattac ctgctcggta tcctgaccac tacgccaggc
    12481 acagatacca atgtggatca tatcgtcgcg atcgagcatt ttattgttcg gcaaggtatc
    12541 gacggctttg attaactgac tggcgatgct ctctgcctct tttagcgtcc ggtgcggtaa
    12601 cagcgcagca aaatcactgc gatggtaacg cgccagcagt gcgccagggt agcgcatcat
    12661 aaatgtcgat agcagattcg tcagagagaa gaattgttct tcaaccaggc tgtgccccca
    12721 ggtatcactc aacatattga aatccggcag acgaatcatc atcacgatcc cgtgggtacc
    12781 tactttctct tgatcttcca gtaacgttgc taactgatta tcgaaaaaga gtcggttatt
    12841 gaggccggtt ttcatgtcct gggcggcata agagcggatc agcgtatcaa gacggctgtg
    12901 ttgttcgcgt gcgttctgaa tttcacaaag cagcgtatcc agcgcactgc tggttctggg
    12961 cggccattca tagatggttc ccaacacatt agagccacgc tcaccgttta agatacgagt
    13021 agcccgggtt tccagcaatt cttgcccggc aagttgccgt tgtaaccagc gtaccgccag
    13081 gaagagcata agaatgataa agccaatcgc ccccgtgagc ggcgcggtgg tcattaaaga
    13141 atggaaatag ttgcccatcg gatcctgata aaccagacgc agcgacatcc ccggatgctt
    13201 tatcaacgga acgctcagtt cacgaaacag atcgttggtg ccaaccggac gataactacc
    13261 atttctggcc agggtataaa cctgcttatc accatggagc aggtctacac gaacgatatc
    13321 tgccgacatc attaattcgg taatttgtgg ccttaatgtg ctgaagtcat tcgacagtag
    13381 gtgggtatca atcgccgtcg ccaccgcctg aacacgatga ctaaacttat actgaatggc
    13441 gttgtagaaa cttagcgaac agcccagcaa agtcacaaaa attgttaacc cggtgagcag
    13501 cgtgacaaag gccgaaaatt tcgtcgttaa tctcatcctt gtgttaactc cgatagtgag
    13561 gaagcgggca tactagcaaa tcagatttat ctcgcaattt attgcgcttc atcggctttg
    13621 cttttccatt agcgagtata gtcttcagaa attattttcc aatccatcat gcacatgagg
    13681 accacttatg caggcgttac ttttagaaca gcaagacggc aaaactctcg catcagtaca
    13741 gactctggac gaaagtcgcc tgccggaagg cgatgtcacg gttgatgttc actggtcgag
    13801 cctgaactac aaagatgcgc tggcgattac cggtaaggga aaaatcatcc gtaattttcc
    13861 gatgattcct gggatcgatt ttgccggaac tgtacgcacc agcgaagatc cgcgttttca
    13921 tgccggtcag gaggtgttac tcactggctg gggcgttggt gaaaaccact ggggtgggct
    13981 ggcggaacag gcgcgagtga aaggtgactg gctggttgcc atgccgcaag ggctggacgc
    14041 gcgtaaagca atgattatcg gtactgccgg ttttaccgcc atgctgtgtg tgatggcgct
    14101 ggaagatgcc ggtgttcgcc cgcaggatgg ggagattgtc gtgacgggtg ccagtggtgg
    14161 cgtcggcagt accgccgtgg cgctgctgca taagttgggt tatcaggtcg ttgctgtttc
    14221 cggtcgcgaa agtacccatg aatatctgaa aagtttaggt gccagccgta ttcttcctcg
    14281 cgatgagttt gccgaatccc gtcctctgga aaaacaagtc tgggctgggg caattgacac
    14341 cgttggcgac aaagtgctgg caaaagtgct ggcgcaaatg aattacggcg gctgcgtggc
    14401 ggcctgtggt ctggcgggtg gttttactct gccaaccacg gtcatgccat ttattctgcg
    14461 taatgtccgt ttgcaagggg tggattcagt aatgacgcca ccagcacgcc gtgcacaagc
    14521 ctggcagcga ctggtcaccg atttaccgga atcattctat acccaggcgg caaaagagat
    14581 atctctggca gaggcaccga agtttgccga ggccatcatt aataaccaga tccagggtcg
    14641 cacgctggtg aaggttaact aaccatttag cagggaataa taagagaggg aactcatttt
    14701 gaaatcatta ttcaatcagt taacgggaaa agcggttagc cggacagctt tcgtcgaaca
    14761 tcttggttat gaagttgtac aacatcatcc aaactggaaa gtcatgattt cgactgacca
    14821 taaattgatg cgcattgatg ctccaccaaa cagccattat tgatacgcct ccgtcgcctg
    14881 ttaggtttat ggtgctttgc cttggcgacg ctacgcttaa tctgttactg atttctggta
    14941 ccatgggggg aataatctga ttttatttga ttacaaatta accacttaaa cctattgaat
    15001 gcggagcatt gtcagtcggt taattttgcg tgctttagca ttcacatctt tccggacgat
    15061 gcagtgaaaa ttgggtaatc cccagcaacc gctgcgtaat gtcgtctatc ttgtcgcgat
    15121 cctggcatcc ctacattatt tgtggtctgt gaagattatc tcattgcagc ccccatcttc
    15181 gcagggctgg ctttacagcg tttcacctta cgttataaga agttccgtcg atgatggcgc
    15241 taatttcgtg aattgtgcgg cttgttgcaa attacacggt gttgaaggtt atttacatgt
    15301 tagctgttga ttatcttccc tgataagacc agtatttagc tgccaattgc tacgaaatcg
    15361 ttataatgtg cgacctcgtc ctccctgacg cagtttttgc actgcggaaa aggtgacatt
    15421 ggcgcaacga aggtatattt tgttttttgc cggaggatag cagcagatcg ctgcacaatg
    15481 tccgtcaagt ctaacattga cactctgggg caaaatagac cggcgtcccg gcctgctgga
    15541 atttatcgct atgcatacag ctgtcggggc atacgcttta cagacggcgg tgaaacgcct
    15601 gtcacaatca cactaaacaa agagtacgga acccactcat ggatattcgt aagattaaaa
    15661 aactgatcga gctggttgaa gaatcaggca tctccgaact ggaaatttct gaaggtgaag
    15721 agtcagtacg cattagccgt gcagctcctg ccgcaagttt ccctgtgatg caacaagctt
    15781 acgctgcacc tatgatgcag cagccagctc aatctaacgc agccgctccg gcgaccgttc
    15841 cttccatgga agcgccagca gcagcggaaa tcagtggtca catcgtacgt tccccgatgg
    15901 ttggtacttt ctaccgcacc ccaagcccgg acgcaaaagc gttcatcgaa gtgggtcaga
    15961 aagtaaacgt gggcgatacc ctgtgcatcg ttgaagccat gaaaatgatg aaccagatcg
    16021 aagcggacaa atccggtacc gtgaaagcaa ttctggtcga aagtggacaa ccggtagaat
    16081 ttgacgagcc gctggtcgtc atcgagtaac gaggcgaaca tgctggataa aattgttatt
    16141 gccaaccgcg gcgagattgc attgcgtatt cttcgtgcct gtaaagaact gggcatcaag
    16201 actgtcgctg tgcactccag tgcggatcgc gatctaaaac acgtattact ggcagatgaa
    16261 acggtctgta tcggccctgc tccgtcagta aaaagttatc tgaacatccc ggcaatcatc
    16321 agcgccgctg aaatcaccgg cgcagtagca atccatccgg gttacggctt cctctccgag
    16381 aacgccaatt ttgccgagca ggttgaacgc tccggcttta tcttcatcgg cccgaaagca
    16441 gaaaccattc gcctgatggg cgacaaagta tccgcaatcg cggcgatgaa aaaagcaggc
    16501 gttccttgcg taccgggttc tgacggcccg ctgggcgacg atatggataa aaaccgcgcc
    16561 attgctaaac gcattggtta tccggtgatt atcaaagcct ccggcggcgg cggtggtcgc
    16621 ggtatgcgcg tagtgcgcag cgacgctgaa ctggcacaat ccatctccat gacccgtgcg
    16681 gaagcgaaag cagctttcag caacgatatg gtttacatgg agaaatacct ggaaaatcct
    16741 cgccacgtcg agattcaggt actggctgac ggtcagggca acgctatcta tctggcggaa
    16801 cgtgactgct ccatgcagcg ccgccaccag aaagtggtcg aagaagcacc ggctccgggc
    16861 attaccccgg aactgcgtcg ctacatcggc gaacgttgcg ctaaagcgtg tgttgatatc
    16921 ggctatcgcg gtgcaggtac tttcgagttc ctgttcgaaa acggcgagtt ctatttcatc
    16981 gaaatgaaca cccgtattca ggtagaacac ccggttactg aaatgatcac cggcgttgac
    17041 ctgatcaaag aacagctgcg tatcgctgcc ggtcaaccgc tgtcgatcaa gcaagaagaa
    17101 gttcacgttc gcggccatgc ggtggaatgc cgtatcaacg ccgaagatcc gaacaccttc
    17161 ctgccaagtc cgggcaaaat cacccgtttc cacgcacctg gtggttttgg cgtacgttgg
    17221 gaatctcata tctacgcggg ctacaccgta ccgccgtact atgactcaat gatcggtaag
    17281 ctgatttgct acggtgaaaa ccgtgacgtg gcgattgctc gcatgaagaa tgcgctgcag
    17341 gagctgatca tcgacggtat caaaaccaac gttgatctgc agatccgcat catgaatgac
    17401 gagaacttcc agcatggtgg cactaacatc cactatctgg agaaaaaact cggtcttcag
    17461 gaaaaataat ttgtgacgcg cggtgttttc atcgcgctta gcaaagaacg taatcaacgc
    17521 cgggcctgtt aatgctaccc ggcgttttta ttgccgttaa ctaacggtag cctgcaattt
    17581 ccgctttcaa cgcgctaatt tcagcatcgc catcaacaat tcccagctca aagcagtatt
    17641 tctttgtttc ccctggctca ataaagggca gacgcccctg cgctcgttct ttatcacgac
    17701 cgtcaggaaa actattactg acctccagtc ccataacata ttcacccgcg cctaaatttt
    17761 tccactggat aaagttgggt agctgcatgg tgtcgaattt ctcgtaaatt ccccacccca
    17821 gttcggcgtt caccagcgca actttactca tcccctgact gtcgctattc aacgtgagat
    17881 agtaaacctg ctcgtcaaaa ccgggctgtg gggtacaaat ctccagatga ctggttaacc
    17941 cttccgccgc atgcggagtt cgtggcgtta tgcttttagc cgacagcaag atttctgcct
    18001 gtggcgataa taaaggccag ccgtaattaa agtgataaag aatttgcagc ggctggcggg
    18061 tgaaaccatg attagtcacc ttgtcttcaa tgcgcattac attttcgcca taacggcagc
    18121 gaatggtcct ttccagcgtc agattttcac cgaacaatcg tgtttcccgt actttgccat
    18181 tgatgacaaa ttcaatgctg tcgtcggtcc gttctgtgcg ataccccacc tcttctgctg
    18241 gagtcgcagc caaacgcccg tgtaacccca gcgtttctcc ttcatcctca cagggtgtgc
    18301 caatgtagct aagtccgcag gtagtcagta accccgcgta aaagctacgt aaaaacccgt
    18361 tgccctgaga ctcaaaaaaa gcgggggcga caacaccgtt gggggtgata aaagagagcg
    18421 cagtgtcctt gtagctggcc catgcaatat ccagcgcgcg atcttttacc accgtaaatg
    18481 ccagtcccgc accagtacgg acatcaaacg cttctgtacc ctttgctctg ccactacgta
    18541 actcatagtg tttaattccg gcaacctgcg agagatcgcc aatccacgac caaatttcgt
    18601 ttttcattgt gctgcttccc gaatggacat gtcatggcgc tggatgaacg tttcgatttc
    18661 cataaggcga gacgttccct ccattgggcc acgtcgggtt accgccagtg caccgcaggc
    18721 gttggcatat tgtaatgccc gatgggcgtc aaaccctagc tggcggcagg caatccacgc
    18781 gccaccaaag cagtctcctg caccggtcgg gtcaacttct tccaccggat agctctcaac
    18841 atgaaactgc tcattcgcgg agtagtaact ggctccttga ttgccgcgct tcacaatcac
    18901 ctctttaaca ccttcttcaa gaaaaccagc gattgcacgc tccggcgtcg agtgtggcga
    18961 caagagcaac acttccccct cactgggcat atagatatcc gttaactcaa gaacaaagtg
    19021 gagggcatcg cgcatttctg gaatatcaag catttcttta cgaatattcg ggtcgaaaga
    19081 aataacgcca ccatttgctt taacgatggt tactgctttt tttactgcgt ctaccatatg
    19141 gaatgaaaac agggatgaac ccataatatg aaaatgggta caatccttca gaatattttc
    19201 atcaacatgc tgtgccgata atttaccgca agcggcattc ttgatattaa aaataaaatc
    19261 gcgatcgcca gagttatgat aagtaacaaa ggcactaccg gtggcttcca gaggtaagac
    19321 tgaaatcccc ctgatgtcca caccgtcagc agccagacga tgtatattaa tatcaccaaa
    19381 gccatcattc cccacgcagc taatgatgcc gcacggaacg ccaagacgtg tcacctggtc
    19441 gataaaaata gcgggcgcac cactgggata agggccgttc cagatacctg gttgatcaaa
    19501 tggctggccg attttactcg ccataatttc aaccagaatt tctccaattg tgaagacctt
    19561 actcatatga ttaattcctt gaaatgatta gctgcgtttt cttttatttc ttacgtcaaa
    19621 ataaacggct acaagaatga ctaatccttt aaccaccatc tggtaaaaat aaggaacatt
    19681 cattaagttc atgccattat taataacgcc gatggttaat gcgccgatca ccgtccccac
    19741 cacggttcca tagccgccgt tcaggctggt gcctcccagt acagctgcgg caatagcatc
    19801 cagttcgtag ctcaacgcgg aattcggttg cccggaatag aggcgtgagg caaggatcat
    19861 cccgctgata cctgccagca cggctgtaat cataaagact ttaattttca agcgggtgac
    19921 atttatccct gaataaattg ccgcttcttt attaccacca gtgatataaa tttttcgtcc
    19981 aaaggttgtt ttagtaagaa tgaagtggtt tatcagcaat aagatgatta atatccatat
    20041 aggaataggt atatagagaa attctccatt ccccagcgcc agaaaggcat catctttaat
    20101 catgatgggc ataccatcgg tcactatata agcgatacca cggaagatcc ccatagtagc
    20161 aacggtgaca ataaacgaag ggatccccgc tatagccgtc aagccaccat ttatcatccc
    20221 cagagccagt gctgcaataa tggataatgg gaccaataaa acaaatggaa ttccccatga
    20281 agccccaagc gcagcaaatg tgccaatcaa tgcaatggtt gaaccgaccg ataaatcaat
    20341 atcgccaagc aaaagcacat aagtcattcc ataggcggta attgcaatta tcgaaacctg
    20401 aagaataata ttagtaatgt tattggcggt aaaaaaactg tcattaataa aactgaacag
    20461 aatgattaac accaccattc cactgaggat accaccatat tgcctgaaga cttttttaaa
    20521 attatttgca atgggaattc ccgaaggttt cggactggca agagttttct ctgtcattta
    20581 acgtactcct gttgcagcgg tcattacctg tgtctgcgtt aaattttcac atgaataaat
    20641 atcggcaata gttttattac gcattaccac cagacgatcg ctaagtgcca tgacttctgg
    20701 cagttcagag gacaccagaa taattgctgt ccctgccgca gccagttgac gtatcacgtt
    20761 atagatttca aattttgcgc cgacatccac tccgcgcgtt ggctcatcga gcaataaaat
    20821 acgcggagtt ttctccagcc acttcgataa cacgattttc tgttgattac cgccgctcag
    20881 tgctcccact gcctgatgca cagaactcac tttagtgttc attctggcaa ttgattgcag
    20941 cgtcttcgcc tcttcttgtc ggtgattgat gagtcccatc cgcgaagcgt tatattgaaa
    21001 cgccacatgg tgcgtgttca tattgacgtc gtgcatcagc actaaccctt cttccttgcg
    21061 gttttcggtc acaaaagcaa tgccatgatc aatagcctgt ttgggattag cgatcctgac
    21121 ttccttacca tcaatcaaca cagtcccgtg atacgaaacg aggccaaata gcgccttcat
    21181 cacatcggaa cgcccggccc ccaccaggcc aaaaaatccg aggacttccc ccggacgcac
    21241 agcgaaactg acctctttaa aaagcgaagg atgggaaaga ttttttactt ccagttttgc
    21301 aggaacattt tcatccggtt tttccccaag acgtgctggc caaatatttt tcatttcccg
    21361 accaaccatt tgggcaatca agtgctggat atcactgttt ttcttttcat gcgtagcgat
    21421 atactcaccg tcgcggatca ccgaaatatc atctgaaatc cgcataattt catccatgcg
    21481 atgagagata taaataaccg cacagcctcg ctgctttaat ttttcaataa tgttaaacag
    21541 gatctccgct tcattatcac ttagcgaaga tgtcggttca tccataatca cgacatccgc
    21601 tgcatagctt aaggctttag caatttctac cagttgccta tgtgccatcg acatattgcc
    21661 gaccatttcc agagggtcta gtgggatgcc cagttccgcg aggaggttct ggcaatctgc
    21721 gagcattttt ttatcgttaa tgatgccaaa acgtcgcggt tcattattgg cataaatatt
    21781 ttccgccacg gtcatattat tactcaggct cagttcctga aaaataatcg caatccctaa
    21841 ctggcgagaa tgctgcggat tattaatggt gatggtttca ccacgtagtg ttatcgttcc
    21901 ctgggttggc ggataaacac cggccagtat tttcattaat gtcgatttac cggcaccatt
    21961 ctcgcctaat aaagtatgca cccgcccttt acgtaaagag agtgtaatat cttttaatgc
    22021 ctgaacatga ccaaatgtct tcgcaatatg cgaaacatca agaataacat ccgtcataat
    22081 gacctcggtg tcacaacaaa acgccaataa cgttttgttg tgaggtgaag atatttacta
    22141 tttacctgta taaacgccgg gttgtaccgg gatcattttt tctaccggtt taccattgag
    22201 gtgatcaaca gcggcatcaa tggctttagc acccatttga tctggatatt gccgaatcac
    22261 ggccgcaaac gttttttcac tgtcaacggc atcacgcgct tctttcatgc cgtcaaaacc
    22321 aatgattttg atgttgtcgt tatgcgcaga ttttgctgcg ataaccgctg ccagcgccgc
    22381 atcatcacca aagccaaaca gaccattcag gttagcattt gcctgcataa tgttttgcgc
    22441 agtcgttaac gcttcagctc gcgtaatacc aggctgttca gcaacaattt taatatccgg
    22501 tgtatcgctt aaccctttct taaaaccatc aacgcgcgca accaccgatt gtagtgccgg
    22561 ataagaaata atggctacag tacctttacc actcaaaagt ttacccatta acttgcctgc
    22621 ttcaacgcct cccgcatagt tatcggtcgc aacatgcgat acgaccggaa cgccttccgc
    22681 agcgacgtca acggtgataa cgggaatacc ggccttatcg gctttaatta tcgctgcctg
    22741 gacgcctttc gaatcgaccg gagagataat aatggcatcc acttttttag taataaaatc
    22801 ctcaacatca gaaagttgtt tgtttaaatc ctgattggca atagagacac ttaaattgac
    22861 attgtctttt tttgcctctt ctttcatggc attcgcaaga tcaatataga aaggatgttg
    22921 ttgtgttaat aatgacgcac caattttata ctcttttgcc aacgaacttt gcgagaacaa
    22981 tgccaggctc accaacatag ctgaaccaag taatttaata tttttcataa acaatcctca
    23041 ttatttttgt agggtacaac gtcttaaatt tttccttcac taccacaaac gccaataata
    23101 cgagttacga cttctttcat ggcggccttt gcgggctgca tatagtgacg tggatcattt
    23161 gccgcaggat tgttgtagaa atatcctttt aacgcatcgg caaacgcaat tttcaattct
    23221 gtcgcgacat tcactttaca aataccgcga cgaatacagt gctgaacgtc ctgttccgat
    23281 aagcccgaag caccatgcaa caccagcgga atctccactc gctgacgaat ctgttccagc
    23341 cgcacaaaat ccagtcgtgg ttcatgcgca tataaaccat gtgctgtgcc gattgctacc
    23401 gccagggagt caatttgggt acgttgaaca aactctgtag ctgcggctgg cgaggtataa
    23461 gcggaatctt tttcatccac ccgcagatcg tcctcctggc cgccaagacg ccctagctcg
    23521 gcctcaacgc taacatcaaa gcgatggcaa aaggtggtaa cttcttgcac tcgcgcaatg
    23581 ttgtcttcaa atggcaaatg ggagccatca atcatgacag aacggatccc tgcgcgaacc
    23641 ttgttaaaaa tgtcatcact ctcttcatga tggtcgagat gcaggactaa cggcaggttg
    23701 tactcgcgag ccagcgattg tactaacgaa attaaacttc ccacgccacc ataacgatat
    23761 gtaccgggtg ttcccgccag cataacggga gagcgaagtt gtgcagcgct ctcaacaact
    23821 acctgtaaag tttcaaggtt atgaatgttg aaagcaggga cggcatatcc ctctgactgg
    23881 gcttttttca gcatgttttt gcttgaaata atgtacatcg cagctcctgt tatttgtcgt
    23941 tcgccttaca aaatgaagaa agagaatctt cactttcatt ttgaaagcaa actagcacag
    24001 caaaagcagc aaaaagcgtg atgcatgtcg aacatcatca aaataactta caaaaaataa
    24061 cttaatttat ttttcacttt gcaagtgaac ccaatgaaaa ccctgcataa atatgaatat
    24121 attcatttat ttttcaatgt attaagatat tttagctgca attttgaccc atgattttct
    24181 tttcgaattg tcgatatgat tgaaggataa ctttcaaaga aaaatacaag gggctatagt
    24241 gttacgcatt aaaggccaat gaagagtgtt aacgtgccga gaaaatcgtc taacttactg
    24301 aaacgtagat taaaaattgc agaaattgtt gccgcccagg gtgaaatcaa agttgatgac
    24361 ttatctgcgc aacttggtgt ctctggcgtg acgatccgcg gcgatctcag ttatctggag
    24421 caacagggat atttaaagcg atcatttggc ggtgctattg cgacaccact ctcctctgaa
    24481 ccagaagcga tacaaccgat cgcgccattg tctctggcac aacaaatgga aatagcacga
    24541 cattgcgcac ggttaattcg cgatcgtgac acgctatttc ttggacatgg gacaatctgc
    24601 cgtaagatca ttccattgct cagcgaagtg aaaaaactgc gtctgatcac caacgatccc
    24661 gaacatgcgc tgctggcaaa tcagtttatt gatggcgaaa taatactggc tggcagtgaa
    24721 atgctgcgcc ccgacaccgc actggccgga aaacagcttg aacaggcact tcagcatttc
    24781 accattacac atagcattct ggaaatcagc catctggatg ctgatggtac tttaaatatc
    24841 aacgttccca gactcgccgc tgcctggcag ctctgtttcg atcatgccca gaataagacg
    24901 gttatcgttg ccgccgatcc tgcgccatca acggctgcta tcgggcattt gcatcaggca
    24961 gaaaatgtga tcgtcagccg cagcattaac gaacaatatc agcaacaatt gcagtcggct
    25021 gatttcgtta tcctttatac cagtaatgag tgctttacct ggtccaatcg cgaaaggctt
    25081 caggaatgag aggggttttg tgacaataac tatctgcgct atgggcgaat tgctggccga
    25141 gtttttgtcc cgcaacccac atcaaaaatt cactcagcct ggggagttta tcgggccatt
    25201 tcccagcggt gcgccagcaa tttttgctgc tcaggtggca aaactgtccc atcgggccat
    25261 cttctttgga tgtgttggta atgatgattt tgcccgactc attatagagc gtctccgtca
    25321 tgaaggtgtc attaccgatg ggatccatgt tatgaacaat gccgtcacag gtacggcgtt
    25381 cgtgagttat caaaatcccc agctgcggga tttcgtcttt aatatcccta acagcgcctg
    25441 cggtttgttt actgccgagc acattgataa ggatctgctt aaacagtgta accatctgca
    25501 tattgtgggc tcatcgttgt tctcatttcg catgatcgat gtcatgcgta aagcaataac
    25561 gacgatcaaa tcggctggcg gcaccgtttc tttcgatccc aatattcgca aagagatgct
    25621 gagcattcct gaaatggcgc aggctctcga ttatttgatt gaatatacgg atatttttat
    25681 ccccagcgaa agcgaactcc ctttcttcgc gcgtcacaaa aatctgtcag aggaacagat
    25741 tgttagcgat cttctccacg gcggcgtaaa acatgtggcg ataaaacgcg cccagcgtgg
    25801 ggctagctat tacaagctta aaaacggtac attacacgcc cagcatgttg caggtcacga
    25861 tatcgaaatt atcgatccaa cgggtgcagg cgactgcttt ggcgcaacgt ttatcactct
    25921 tttcttatcc ggtttcccgg cacacaaggc gctgcaatat gcaaatgcca gcggcgcgct
    25981 cgccgtaatg cggcaaggtc cgatggaagg gatatcctca ctggcagaca ttgaagactt
    26041 tttgcagcag cactgaacgg caattttctg tcagattatt ttccctgcct gaagacttgc
    26101 cgtagaattg cccgtttctc gtatcccccc atgaggagga ccccttccgt ggacactcgt
    26161 tttgttcagg cccataaaga ggcgcgctgg gcgctggggc tgaccctttt gtatctggcg
    26221 gtatggttag tagccgctta cttacctggc gttgcccccg gttttaccgg ttttccgcgc
    26281 tggtttgaga tggcctgcat cctgacgccg ctgctgttta ttggactgtg ctgggcgatg
    26341 gtgagattta tctatcgcga tatcccactg gaggatgacg atgcagcttg aagtaattct
    26401 accgctggtc gcctatctgg tggtggtgtt cggtatctct gtttatgcga tgcgtaaacg
    26461 gagcactggc accttcctta atgagtattt cctcggcagc cgctctatgg gcggtattgt
    26521 gctggcgatg acgctcaccg cgacctatat cagtgccagt tcgtttatcg gcgggcctgg
    26581 agctgcttat aaatacggcc tgggctgggt attgctggcg atgattcagc ttcctgcagt
    26641 ctggctttcg ctcggtattc tcggcaagaa gttcgcaatt cttgcgcgcc gctacaatgc
    26701 ggtgacgctg aacgatatgc tgtttgcccg ctaccagagt cgtcttctgg tgtggctggc
    26761 gagtttgagt ttgctggttg cgttcgttgg tgcgatgacc gtgcagttta tcggcggtgc
    26821 gcgcctgctg gaaaccgcgg cgggtattcc ttatgaaacc gggctgctga ttttcggtat
    26881 cagcattgcg ttgtataccg cctttggcgg ctttcgcgcc agcgtgctga acgacaccat
    26941 gcaagggctg gtgatgctga ttggcaccgt tgtgctgctt attggcgtgg tacatgccgc
    27001 aggtggttta agtaatgcgg tagacacgct gcaaaccatc gatccgcaac tggttacgcc
    27061 acaaggcgct gacgatattc tgtcgcctgc ctttatgacg tcgttctggg tactggtgtg
    27121 ctttggcgtt ataggcctgc cgcatactgc ggtgcgctgt atctcttata aagacagcaa
    27181 agcagtacat cgggggatca tcatcggtac gattgtagtc gcaatcctga tgttcggtat
    27241 gcatctggcc ggagcgttag gtcgggcggt gatccccgat ctcaccgtac cggatctggt
    27301 gatcccgacg ttaatggtaa aagtgctgcc accgtttgcc gccgggatct tcctggctgc
    27361 accgatggct gcgatcatgt cgacaattaa cgcccaactg ctgcaaagtt ccgctacgat
    27421 cattaaagat ctctatctga atatccgtcc ggatcaaatg caaaacgaga cgcgtctgaa
    27481 gcggatgtcg gcggtgatca cgttggttct cggtgcgttg ctgctgcttg ccgcctggaa
    27541 gccgccagaa atgatcatct ggctgaattt gctggccttc ggtgggctgg aagccgtttt
    27601 cctgtggccg ctggtgctgg gtctttactg ggaacgcgcc aacgccaaag gcgcgctaag
    27661 tgcgatgatc gttggcggcg tgctgtatgc cgtactcgcg aagctgaata ttcagtacct
    27721 gggcttccac cctatcgtgc cctcgttact actaagtttg ctggctttcc tggtcggaaa
    27781 ccgtttcggt acatccgtcc cgcaagctac cgttttgact actgataaat aaagagtttt
    27841 gccatgcctt ggatccaact gaaactgaac accaccggcg caaacgcgga agatcttagc
    27901 gatgcgctga tggaagcggg tgccgtttct atcacttttc aggataccca cgatacgcca
    27961 gtatttgagc cgctgccggg cgaaacgcgc ctgtggggcg acaccgatgt gattggtctg
    28021 ttcgacgctg aaaccgatat gaacgacgtg gtggcgattc tggaaaacca tccgctgctc
    28081 ggcgcgggct tcgcgcataa aatcgaacaa ctggaagata aagactggga gcgcgaatgg
    28141 atggataatt tccacccgat gcgctttggt gaacgactgt ggatctgccc aagctggcgt
    28201 gatgtgccgg acgaaaacgc cgtcaacgtg atgttagacc cagggctggc gtttggtacg
    28261 ggtacccatc caactacctc tctgtgcctg caatggctcg acagcctcga tttaaccggt
    28321 aaaacagtca tcgactttgg ctgcggttcc ggcattctgg ctatcgcggc gctgaaactg
    28381 ggtgcagcaa aagccattgg tattgatatc gatccgcagg cgatccaggc cagccgcgat
    28441 aacgccgaac gtaatggcgt ttctgatcgt ctggaactct acttaccgaa agatcagcca
    28501 gaagaaatga aagccgacgt ggtggtcgct aacatccttg caggcccatt acgtgaactg
    28561 gcaccgttaa tcagcgtcct gccagtttca ggcggtttgc tgggcctttc cggtattctg
    28621 gcaagccagg cagagagcgt ttgcgaagct tatgccgaca gcttcgctct ggacccggtc
    28681 gtggaaaaag aagagtggtg ccgcattacc ggtcgtaaga attaaccttc gcatcgccgt
    28741 atggcgacgc agagcgaagt gcgagcgatg tcacaaaagg cacgttaatt tgccgattat
    28801 ttacgcaaat ttgcg
//
LOCUS       DAHHTG010000055        28764 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.055, whole genome shotgun sequence.
ACCESSION   DAHHTG010000055 DAHHTG010000000
VERSION     DAHHTG010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28764)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28764)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28764
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.055"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            complement(<1..1839)
                     /gene="selB"
                     /locus_tag="NBI74_002329"
     CDS             complement(<1..1839)
                     /gene="selB"
                     /locus_tag="NBI74_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HCD1932653.1"
                     /translation="MIIATAGHVDHGKTTLLQAITGVNADRLPEEKKRGMTIDLGYAY
                     WPQPDGRVPGFIDVPGHEKFLSNMLAGVGGIDHALLVVACDDGVMAQTREHLAILQLT
                     GNPMLTVALTKADRVDEARVDEVERQVKEVLREYGFAEAKLFITAATEGRGIDALREH
                     LLQLPEREHASQHSFRLAIDRAFTVKGAGLVVTGTALSGEVKVGDSLWLTGVNKPMRV
                     RALHAQNQPTETAHAGQRIALNIAGDAEKEQINRGDWLLADVPPEPFTRVIVELQTHT
                     PLTQWQPLHIHHAASHVTGRVSLLEDNLAELVFDTPLWLADNDRLVLRDISARNTLAG
                     ARVVMLNPPRRGKRKPEYLQWLASLARAQSDADALSVHLERGAVNLADFAWARQLNGE
                     GMRELLQQPGYIQAGYSLLNAPVAARWQRKILDTLATYHEQHRDEPGPGRERLRRMAL
                     PMEDEALVLLLIEKMRESGDIHSHHGWLHLPDHKAGFSEELQAIWQKAEPLFGDEPWW
                     VRDLAKETGTDEQAMRLTLRQAAQQGIITAIVKDRYYRNDRIVEFANMIRDLDQECGS
                     TCAADFRDRLGVGRKLAIQILEYFDRIGFTRRRGNDHLLRDALLFPE"
     gene            complement(1836..3227)
                     /gene="selA"
                     /locus_tag="NBI74_002330"
     CDS             complement(1836..3227)
                     /gene="selA"
                     /locus_tag="NBI74_002330"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HCD1932654.1"
                     /translation="MTTETRSLYSQLPAIDRLLRDSSFLSLRDTYGHTRVVELLRQML
                     DEAREVIRDSQTLPAWCENWAQEVDARLTKEAQSALRPVINLTGTVLHTNLGRALQAE
                     AAVEAVTKAMRSPVTLEYDLDDAGRGHRDRALAQLLCRITGAEDACIVNNNAAAVLLM
                     LAATASGKEVVVSRGELVEIGGAFRIPDVMRQAGCTLHEVGTTNRTHANDYRQAVNEN
                     TALLMKVHTSNYSIQGFTKAIDEAELVALGKELDVPVVTDLGSGSLVDLSQYGLPKEP
                     MPQELIAAGVSLVSFSGDKLLGGPQAGIIVGKKEMIARLQSHPLKRALRADKMTLAAL
                     EATLRLYLHPEALSEKLPTLRLLTRSAEVIQIQAQRLQALLAAHYGAEFAVQVMPCLS
                     QIGSGSLPVDRLPSAALTFTPHDGRGSHLESLAARWRELPVPVIGRIYDGRLWLDLRC
                     LEDEQRFLEMLLK"
     gene            complement(3325..3933)
                     /locus_tag="NBI74_002331"
     CDS             complement(3325..3933)
                     /locus_tag="NBI74_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HCD1932655.1"
                     /translation="MKLVGSYTSPFVRKLSILLLEKGITFEFINELPYNADNGVAQFN
                     PLGKVPVLVTEEGECWFDSPIIAEYIELMNVAPAMLPRDPLESLRVRKIEALADGIMD
                     AGLVSVREQARPAAQQSEDELLRQREKINRSLDVLEGYLVDGTLKTDTVNLATIAIAC
                     AVGYLNFRRVAPGWCVDRPHLVKLVENLFSRESFARTEPPKA"
     gene            complement(4004..5140)
                     /locus_tag="NBI74_002332"
     CDS             complement(4004..5140)
                     /locus_tag="NBI74_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HCD1932656.1"
                     /translation="MDLLIVLTYVALAWAVFKIFRIPVNQWTLATAALGGVFLVSGLI
                     LLMNYNHPYTFTAQKAVIAIPITPQVTGIVTEVTDKNNQLIQKGEVLFKLDPVRYQAR
                     VDRLQADLMTATHNIKTLRAQLTEAQANTTQVSAERDRLFKNYQRYLKGSQAAVNPFS
                     ERDIDDARQNFLAQDALVKGSVAEQAQIQSQLDSMVNGEQSQIVSLRAQLTEAKYNLE
                     QTVIRAPSNGYVTQVLIRPGTYAAALPLRPVMVFIPEQKRQIVAQFRQNSLLRLKPGD
                     DAEVVFNALPGQVFHGKLTSILPVVPGGSYQAQGVLQSLTVVPGTDGVLGTIELDPNA
                     DIDALPDGIYAQVAVYSDHFSHVSVMRKVLLRMTSWMHYLYLDH"
     gene            complement(5143..5505)
                     /locus_tag="NBI74_002333"
     CDS             complement(5143..5505)
                     /locus_tag="NBI74_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HCD1932657.1"
                     /translation="MFLNYFALGVLIFVFLVIFYGIIAIHDIPYLIAKKRNHPHADAI
                     HTAGWVSLFTLHVIWPFLWIWATLYQPERGWGMQSHVASQEKATDPEIAALSDRISRL
                     EHQLAAEKKTDYSTFPEI"
     gene            6042..7955
                     /gene="mtlA"
                     /locus_tag="NBI74_002334"
     CDS             6042..7955
                     /gene="mtlA"
                     /locus_tag="NBI74_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HCD1932658.1"
                     /translation="MSSDIKIKVQSFGRFLSNMVMPNIGAFIAWGIITALFIPTGWLP
                     NETLAKLVGPMITYLLPLLIGYTGGKLVGGERGGVVGAITTMGVIVGADMPMFLGSMI
                     AGPLGGWCIKHFDRWVDGKIKSGFEMLVNNFSAGIIGMILAILAFLGIGPIVEALSKM
                     LAAGVNFMVVHDMLPLASIFVEPAKILFLNNAINHGIFSPLGIQQSHELGKSIFFLIE
                     ANPGPGMGVLLAYMFFGRGSAKQSAGGAAIIHFLGGIHEIYFPYVLMNPRLILAVILG
                     GMTGVFTLTILGGGLVSPASPGSILAVLAMTPKGAYFANIAGVCAAMAVSFVVSAILL
                     KTSKVKEEDDIEAAARRVQEMKAESKGASPLSAGDVTNDLSHVRKIIVACDAGMGSSA
                     MGAGVLRKKIQDAGLSQISVTNSAINNLPPDVDLVITHRDLTERAMRQVPQAQHISLT
                     NFLDSGLYTSLTERLVAAQRHTENEVKVKDSLKDSFDDSSANLFKLGAENIFLGRKAA
                     TKEEAIRFAGEQLVKGGYVEPEYVQAMLDREKLTPTYLGESIAVPHGTVEAKDRVLKT
                     GVVFCQYPEGVRFGEEEDDIARLVIGIAARNNEHIQVITSLTNALDDESVIERLAHTT
                     SVDEVLELLAGRK"
     gene            8275..9423
                     /gene="mtlD"
                     /locus_tag="NBI74_002335"
     CDS             8275..9423
                     /gene="mtlD"
                     /locus_tag="NBI74_002335"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HCD1932659.1"
                     /translation="MKALHFGAGNIGRGFIGKLLADAGIQLTFADVNQVVLDALNARH
                     SYQVHVVGETEQVDTVSGVDAVSSIGDDVVDLIAQVDLVTTAVGPVVLERIAPAIAKG
                     LVKRKEQGNESPLNIIACENMVRGTTQLKGHVMNALPEDAKAWVEEHVGFVDSAVDRI
                     VPPSASATNDPLEVTVETFSEWIVDKTQFKGTLPNIPGMELTDNLMAFVERKLFTLNT
                     GHAITAYLGKLAGHQTIRDAILDEKIRAVVKGAMEESGAVLIKRYGFDADKHAAYIQK
                     ILGRFENPYLKDDVERVGRQPLRKLSAGDRLIKPLLGTLEYGLPHKNLIEGIAAAMHF
                     RSEDDPQAQELAALIADKGPQAALAQISGLDANSEVVSEAVTAYKAMQ"
     gene            9423..10010
                     /gene="mtlR"
                     /locus_tag="NBI74_002336"
     CDS             9423..10010
                     /gene="mtlR"
                     /locus_tag="NBI74_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol operon repressor MtlR"
                     /protein_id="HCD1932660.1"
                     /translation="MVDQAQDTLRPNNRLSDMQATMEQTQAFENRVLERLNAGKTVRS
                     FLITAVELLTEAVNLLVLQVFRKDDYAVKYAVEPLLDGDGPLGDLSVRLKLIYGLGVI
                     NRQEYEDAELLMALREELNHDGNEYAFTDDEILGPFGELHCVAALPPPPQFEPADSSL
                     YAMQIQRYQQAVRSTMVLSLTELISKISLKKAFQK"
     gene            complement(10021..10230)
                     /locus_tag="NBI74_002337"
     CDS             complement(10021..10230)
                     /locus_tag="NBI74_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1932661.1"
                     /translation="MGKLGENVPLLIDKAVDFMASSQAFREYLKKLPPRNAIPSGIPD
                     ESVPLYLQRLEYYRQLYRPKQVEEK"
     gene            10514..10876
                     /locus_tag="NBI74_002338"
     CDS             10514..10876
                     /locus_tag="NBI74_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibL family ribosome-associated protein"
                     /protein_id="HCD1932662.1"
                     /translation="MKEVEKNEIKRLSDRLDAIRHQQADLSLVEAADKYAELEKEKAT
                     LEAEIARLREVHSQKLSKEAQKLMKMPFQRAITKKEQADMGKLKKSVRGLVVVHPMTA
                     LGREMGLQEMTGFSKTAF"
     gene            11420..12100
                     /locus_tag="NBI74_002339"
     CDS             11420..12100
                     /locus_tag="NBI74_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HCD1932663.1"
                     /translation="MSMKTLLNVLTIFSLGTLTGCDYIEKASLIDDLAKQQELQKSKI
                     EELEKQQEQYKNKAELFEKQQNNITNSTRILASIVKTVKEKQDEFVFTEFNPLQTQYF
                     ILNNGSVGLAGRVLAIDAVENGSVIRISLVNLLSVPVSNIGFHATWGNEKPTDAKALA
                     KWQQLLFNTTLNSTLQLMPGQWQDINLTLKGVSPNNLKYLKLSINMANLQFDTVQPAE
                     TRQRKNKK"
     gene            12144..16487
                     /locus_tag="NBI74_002340"
     CDS             12144..16487
                     /locus_tag="NBI74_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YadA-like family protein"
                     /protein_id="HCD1932664.1"
                     /translation="MNKIFKVIWNPATGSYNVASETAKSRGKKSGRSKLLISALVAGG
                     LLSSFGALADNYEGQPVDYGNGSAGDGWVAIGKNSKANTFVNGSGASTALGYDAIAEG
                     EYSSAIGSRTHAIGGASMAFGVSAQAIGDRSVALGASSVATGGRSMALGRYAKTNGFT
                     SLAIGDTALADGEKTIALGNTAQAYEIMSIALGDHANASKEYSMALGASSVASAANAI
                     AVGRDSAANGTDSLALGRLSLASAANAIAMGAESEAAENATAIGFNADAIGKSSLALG
                     DNASAGETNSIAIGQGSEASKLDSIALGSNSRSAGENAIALGNNSNAGGKNSLAFGFN
                     TTANGDNAVAIGANSSAGADNTVSVGSSSLKRKIVNMGNGDINNVSSDAINGSQLYAI
                     SKSVSDRLGGYHDDPDNVINSDGTLKAPTYYLQSGQYNNVGEALQSIDNNTLHWDSKS
                     NKYSASHTVFNANGSVKSTSAKNIITDVADGTISATSSDAVNGSQLYNLKQDALLWDG
                     TAFSAKHGTSNTNSKITNVADGAVSASSKDAVNGSQLYDLKQDALLWDGTAFSAKHGT
                     SNTDSKITNVLAGTVSSTSTDAINGTQLQSTASTITSYLGGGAAMSNDGVFTGPQYNI
                     GGKNYTNVGAALTALDNDALLWDATANSNTGAFSAAHGTLKTASKITNVANGDILANS
                     YDAINGSQLYSLADSFTSYLGGGADINNAGVLIAPTYTIGSKTYNNVGDALNAINSSF
                     STSISDALLWDSTANKFSAKHGGTNSIITNVADGTISSTSSDAVNGSQLYDTSKYIAD
                     ALGGDAEVNADGTMTAPTYSIANEDYHSVGEALDALDDNALLWDEDAGAYNASHDGNA
                     SKITNVAAGDLSTTSTDAVNGSQLNATNILVTQNSQMINQLAGNTSETYIEENGAGIN
                     YVRTNDNGLAFNDASASGIGATAVGYNSVAEGDSSVAIGQGSSSSVDTGIALGSSSVS
                     SRVIVKGSRNTSVTENGVVIGYDTTDGELLGALSIGDDGKYRQIINVADGSEAHDAVT
                     VRQLQNAIGAVATTPTKYFHANSTAEDSLAVGEDSLAMGAKTIVNGNAGIGIGLNTLV
                     LADAINGIAIGSNARANHADSIAMGNGSQTTRGAQTNYTAYNMDAPQNSVGEFSVGSE
                     DGQRQITNVAAGSADTDAVNVGQLKVTDAQVSQNTQSITNLNTQVTNLDTRVTNIENG
                     IGDIVTTGSTKYFKTNTDGADANAQGKDSVAIGSGSIAAADNSVALGTGSVADEENTI
                     SVGSSTNQRRITNVAAGVNATDAVNVSQLKSSEAGGVRYDTKADGSIDYSNITLGGGN
                     GGTTRISNVSAGVNNNDAVNYAQLKQSVQETKQYTDQRMVEMDNKLSKTESKLSGGIA
                     SAMAMTGLPQAYTPGASMASIGGGTYNGESAVALGVSMVSANGRWVYKLQGSTNSQGE
                     YSAALGAGIQW"
     gene            16905..18560
                     /gene="lldP"
                     /locus_tag="NBI74_002341"
     CDS             16905..18560
                     /gene="lldP"
                     /locus_tag="NBI74_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HCD1932665.1"
                     /translation="MNLWQQNYDPAGNIWLSSLIASLPILFFFFALIKLKLKGYVAAS
                     WTVAIALAVALLFYKMPVANALASVVYGFFYGLWPIAWIIIAAVFVYKISVKTGQFDI
                     IRSSILSITPDQRLQMLIVGFCFGAFLEGAAGFGAPVAITAALLVGLGFKPLYAAGLC
                     LIVNTAPVAFGAMGIPILVAGQVTGIDSFEIGQMVGRQLPFMTIIVLFWIMAIMDGWR
                     GIKETWPAVVVAGGSFAIAQYLSSNFIGPELPDIISSLVSLLCLTLFLKRWQPVRVFR
                     FGDLGASQVDMTLAHTGYTAGQVLRAWTPFLFLTATVTLWSIPPFKALFTSGGALYEW
                     VINIPVPYLDKLVARMPPVVSEATAYAAVFKFDWFSATGTAILFAALLSIVWLKMKPS
                     DAISTFGSTLKELALPIYSIGMVLAFAFISNYSGLSSTLALALAHTGHAFTFFSPFLG
                     WLGVFLTGSDTSSNALFAALQATAAQQIGVSDLLLVAANTTGGVTGKMISPQSIAIAC
                     AAVGLVGKESDLFRFTVKHSLIFTCMVGVITTLQAYVLTWMIP"
     gene            18560..19336
                     /gene="lldR"
                     /locus_tag="NBI74_002342"
     CDS             18560..19336
                     /gene="lldR"
                     /locus_tag="NBI74_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LldR"
                     /protein_id="HCD1932666.1"
                     /translation="MIVLPRRLSDEVADRVRALIDEKNLEAGMKLPAERQLAMQLGVS
                     RNSLREALAKLVSEGVLLSRRGGGTFIRWRHDTWSEQNIVQPLKTLMADDPDYSFDIL
                     EARYAIEASTAWHAAMRATPGEKEKIQLCFEATLSEDPDLASQADVRFHLAIAEASHN
                     IVLLQTMRGFFDVLQSSVKHSRQRMYLVPPVFSQLTEQHQAVIDAIFVGDADGARKAM
                     MAHLSFVHTTMKRFDEDQARHARITRLPGDHNEHSREKNA"
     gene            19333..20523
                     /gene="lldD"
                     /locus_tag="NBI74_002343"
     CDS             19333..20523
                     /gene="lldD"
                     /locus_tag="NBI74_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent L-lactate dehydrogenase"
                     /protein_id="HCD1932667.1"
                     /translation="MIISAASDYRAAAQRILPPFLFHYMDGGAYSEYTLRRNVEDLSE
                     VALRQRILKNMSDLSLETTLFNEKLSMPVALGPVGLCGMYARRGEVQAAKAADAHGIP
                     FTLSTVSVCPIEEVAPAIKRPMWFQLYVLRDRGFMRNALERAKAAGCSTLVFTVDMPT
                     PGARYRDAHSGMSGPNAAMRRYLQAVTHPQWAWDVGLNGRPHDLGNISAYLGKPTGLE
                     DYIGWLGNNFDPSISWKDLEWIRDFWDGPMVIKGILDPEDARDAVRFGADGIVVSNHG
                     GRQLDGVLSSARALPAIADAVKGDIAILADSGIRNGLDVVRMIALGADTVLLGRAFLY
                     ALATAGQAGVANLLNLIEKEMKVAMTLTGAKSISEITQDSLVQGLGKELPAALAPMAK
                     GNAA"
     gene            20571..21044
                     /gene="trmL"
                     /locus_tag="NBI74_002344"
     CDS             20571..21044
                     /gene="trmL"
                     /locus_tag="NBI74_002344"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HCD1932668.1"
                     /translation="MLNIVLYEPEIPPNTGNIIRLCANTGFRLHIIEPMGFAWDDKRL
                     RRAGLDYHEFTAVTRHHDYRAFLEAENPQRLFALTTKGTPAHSAVSYQDGDYLMFGPE
                     TRGLPASILDALPAEQKIRIPMVPDSRSMNLSNAVSVVVYEAWRQLGYPGAVLRD"
     gene            complement(21097..21918)
                     /gene="cysE"
                     /locus_tag="NBI74_002345"
     CDS             complement(21097..21918)
                     /gene="cysE"
                     /locus_tag="NBI74_002345"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HCD1932669.1"
                     /translation="MSCEELEIVWNNIKAEARTLADCEPMLASFYHATLLKHENLGSA
                     LSYMLANKLSSPIMPAIAIREVVEEAYAADPEMIASAACDIQAVRTRDPAVDKYSTPL
                     LYLKGFHALQAYRIGHWLWNQGRRALAIFLQNQVSVTFQVDIHPAAKIGRGIMLDHAT
                     GIVVGETAVIENDVSILQSVTLGGTGKSGGDRHPKIREGVMIGAGAKILGNIEVGRGA
                     KIGAGSVVLQPVPPHTTAAGVPARIVGKPDSDKPSMDMDQHFNGINHTFEYGDGI"
     gene            complement(21998..23017)
                     /gene="gpsA"
                     /locus_tag="NBI74_002346"
     CDS             complement(21998..23017)
                     /gene="gpsA"
                     /locus_tag="NBI74_002346"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HCD1932670.1"
                     /translation="MNQRNASMTVIGAGSYGTALAITLARNGHEVVLWGHDPEHIATL
                     ERDRCNAAFLPDVPFPDTLHLESDLATALAASRNILVVVPSHVFGEVLRQIKPLMRPD
                     ARLVWATKGLEAETGRLLQDVAREALGDQIPLAVISGPTFAKELAAGLPTAISLASTD
                     QTFADDLQQLLHCGKSFRVYSNPDFIGVQLGGAVKNVIAIGAGMSDGIGFGANARTAL
                     ITRGLAEMSRLGAALGADPATFMGMAGLGDLVLTCTDNQSRNRRFGMMLGQGMDVQSA
                     QEKIGQVVEGYRNTKEVRELAHRFGVEMPITEEIYQVLYCGKNAREAALTLLGRARKD
                     ERSSH"
     gene            complement(23017..23484)
                     /gene="secB"
                     /locus_tag="NBI74_002347"
     CDS             complement(23017..23484)
                     /gene="secB"
                     /locus_tag="NBI74_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015369180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HCD1932671.1"
                     /translation="MSEQNNTEMTFQIQRIYTKDISFEAPNAPHVFQKDWQPEVKLDL
                     DTASTQLADDVYEVVLRVTVTASLGEETAFLCEVQQGGIFSIAGIEGTQMAHCLGAYC
                     PNILFPYARECITSMVSRGTFPQLNLAPVNFDALFMNYLQQQAGEGTEEHQDA"
     gene            complement(23548..23799)
                     /gene="grxC"
                     /locus_tag="NBI74_002348"
     CDS             complement(23548..23799)
                     /gene="grxC"
                     /locus_tag="NBI74_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HCD1932672.1"
                     /translation="MANVEIYTKETCPYCHRAKALLSSKGVSFQELPIDGNAAKREEM
                     IKRSGRTTVPQIFIDAQHIGGCDDLYALDARGGLDPLLK"
     gene            complement(23940..24371)
                     /locus_tag="NBI74_002349"
     CDS             complement(23940..24371)
                     /locus_tag="NBI74_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HCD1932673.1"
                     /translation="MQEIMQFVGRHPILSIAWIALLVAVLVTTFKSLTSKVKVITRGE
                     ATRLINKEDAVVVDLRQRDDFRKGHIAGSINLLPSEIKANNVGELEKHKDKPVIVVDG
                     SGMQCQEPANALTKAGFAQVFVLKEGVAGWAGENLPLVRGK"
     gene            24615..26159
                     /gene="gpmM"
                     /locus_tag="NBI74_002350"
                     /gene_synonym="pgmI"
     CDS             24615..26159
                     /gene="gpmM"
                     /locus_tag="NBI74_002350"
                     /gene_synonym="pgmI"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HCD1932674.1"
                     /translation="MSVSKKPMVLVILDGYGYREEQQDNAIFSAKTPVMDALWANRPH
                     TLIDASGLEVGLPDRQMGNSEVGHVNLGAGRIVYQDLTRLDVEIKDRTFFANPVLTGA
                     VDKAKNAGKAVHIMGLLSAGGVHSHEDHIMAMVELAAERGAEKIYLHAFLDGRDTPPR
                     SAESSLKKFEEKFAALGKGRVASIIGRYYAMDRDNRWDRVEKAYDLLTLAQGEFQADT
                     AVAGLQAAYARDENDEFVKATVIRAEGQPDAAMEDGDALIFMNFRADRAREITRAFVN
                     ADFDGFARKKVVNVDFVMLTEYAADIKTAVAYPPASLVNTFGEWMAKNDKTQLRISET
                     EKYAHVTFFFNGGVEESFKGEDRILINSPKVATYDLQPEMSSAELTEKLVAAIKSGKY
                     DTIICNYPNGDMVGHTGVMEAAVKAVEALDHCVEEVAKAVESVGGQLLITADHGNAEQ
                     MRDPATGQAHTAHTNLPVPLIYVGDKNVKAVEGGKLSDIAPTMLSLMGMEIPQEMTGK
                     PLFIVE"
     gene            26169..27452
                     /gene="envC"
                     /locus_tag="NBI74_002351"
     CDS             26169..27452
                     /gene="envC"
                     /locus_tag="NBI74_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator EnvC"
                     /protein_id="HCD1932675.1"
                     /translation="MRGKAINTMTRAVKPRRFAIRPIIYASVLSAGVLLCAFSAHADE
                     RDQLKSIQADIAAKERAVRQKQQQRASLLAQLKKQEEAISEATRKLRETQNTLNQLNK
                     QIDEMNASIAKLEQQKAAQERSLAAQLDAAFRQGEHTGIQLILSGEESQRGQRLQAYF
                     GYLNQARQETIAQLKQTREEVAMQRAELEEKQSEQQTLLYEQRAQQAKLTQALSERKK
                     TLAGLESSIQQGQQQLSELRANESRLRNSIARAEAAAKARAEREAREAQAVRDRQKEA
                     TRKGTTYKPTESEKSLMSRTGGLGAPRGQAFWPVRGPTLHRYGEQLQGELRWKGMVIG
                     ASEGTEVKAIADGRVILADWLQGYGLVVVVEHGKGDMSLYGYNQSALVSVGSQVRAGQ
                     PIALVGSSGGQGRPSLYFEIRRQGQAVNPQPWLGR"
     gene            27456..28415
                     /locus_tag="NBI74_002352"
     CDS             27456..28415
                     /locus_tag="NBI74_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent polysaccharide deacetylase family
                     protein"
                     /protein_id="HCD1932676.1"
                     /translation="MFPFRRNVLAFAALLALSSPVIAGKLAIVIDDFGYRPHNENQVL
                     AMPSAISVAVLPDSPHAREMATKAHNSGHEVLIHLPMAPLSKQPLEKNTLRPEMSSDE
                     IERIIRSAVNNVPYAVGINNHMGSKMTSNLFGMQKVMQALERYNLYFLDSVTIGNTQA
                     MRAAQGTGVKVIKRKVFLDDSQNEADIRVQFNRAIDLARRNGSTIAIGHPHPSTVRVL
                     QQLVYNLPPDITLVKASSLLNEPQVDTSTPPKNTVPDTPRNPFRGVKLCKPKKPLEPV
                     YANRFFEVLSESISQSTLIVYFQHQWQGWGKQPEAAKLNASAN"
     gene            complement(28402..>28764)
                     /gene="waaH"
                     /locus_tag="NBI74_002353"
     CDS             complement(28402..>28764)
                     /gene="waaH"
                     /locus_tag="NBI74_002353"
                     /EC_number="2.4.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucuronate:LPS(HepIII) glycosyltransferase"
                     /protein_id="HCD1932677.1"
                     /translation="LHRQGNKNLNYQRHYIKITRLLEKLNRNYADKITIYPEFHQQIT
                     YEALRVCHAVRKEPDILTRQRMIAEIFTSGMYKRLITNVRSVKVGYQALLWSFRLWQW
                     RDKTRSHHRITRSAFNLR"
BASE COUNT         7078 a         7558 c         7443 g         6685 t
ORIGIN      
        1 ttccggaaat aataatgcgt cgcgcaataa atgatcattt ccacgacgac gcgtaaagcc
       61 aatgcggtca aaatattcca gaatctgaat tgccagcttg cggcctacgc ctaagcgatc
      121 gcggaaatcc gccgcgcagg ttgaaccaca ttcctgatcg agatcgcgga tcatattggc
      181 aaattcgaca atccgatcgt tacggtaata acgatctttg acgatcgcag taattatccc
      241 ctgttgggcc gcctggcgta gcgtcaggcg cattgcctgc tcgtcggttc ccgtctcttt
      301 tgccaggtca cgcacccacc atggctcgtc accaaacagc ggctctgctt tttgccagat
      361 ggcctgcagc tcttcgctga agcccgcttt atgatccggc agatgcagcc agccgtgatg
      421 gctgtggatg tcgccgcttt cgcgcatctt ttcaatcagc aacagtacca gcgcttcatc
      481 ttccattggc aacgccatac gtcgcagacg ttcgcgccca ggacccggtt catcgcgatg
      541 ttgctcatga taagtcgcta atgtgtcgag aattttccgc tgccagcggg cggcaactgg
      601 cgcattcaac aagctgtaac cagcctgaat atagccaggc tgttgcagca gttcgcgcat
      661 cccttcgccg ttgagctgcc gtgcccaggc gaaatccgca aggttaaccg cgccgcgttc
      721 cagatgaaca gataacgcat cggcatcgct ctgcgcccgt gcaagagacg ccagccattg
      781 cagatattcc ggcttacgct taccgcgacg cggcgggtta agcatcacca cacgcgcccc
      841 cgccagcgtg ttgcgggccg agatatcgcg cagcactaag cgatcgttat ccgccagcca
      901 cagcggcgtg tcgaacacca gctcggcaag gttatcttcc agcagcgaaa cgcgtcccgt
      961 aacgtggctg gcggcgtggt gaatatgcag cggctgccac tgggtcagcg gtgtatgggt
     1021 ttgaagctcg acgatcaccc gtgtgaacgg ctccggcggc acatcggcaa gcagccagtc
     1081 gccacggtta atctgctctt tttccgcatc acccgcgatg ttaagcgcga tacgctgccc
     1141 ggcatgggca gtttctgttg gctgattttg cgcatgcagc gcacgcacgc gcatcggttt
     1201 atttacacca gtcagccaga gtgaatcgcc tactttcact tccccgctta acgccgtacc
     1261 ggtgacgacc agcccggcac cttttacggt aaatgcgcgg tcgatcgcga ggcggaaact
     1321 atgttggctg gcgtgttcac gttctggtaa ctgaagcaga tgctcgcgca gggcatcaat
     1381 tccccgacct tcggttgctg cggtgataaa cagttttgcc tcagcaaaac cgtattcccg
     1441 cagcacctcc tttacctggc gttcaacctc atcaacacgc gcttcgtcca cgcgatcggc
     1501 tttggtcagc gccactgtca gcatcgggtt accggtcagc tgcaaaatcg ccagatgctc
     1561 acgggtctgt gccatcacac cgtcatcgca cgccaccacc aacagcgcgt gatcgatacc
     1621 accaacgccc gccagcatgt tggaaagaaa cttttcatga ccgggtacgt cgataaaacc
     1681 aggcacgcga ccatccggct gcggccagta ggcatagccg agatcgatgg tcatgccgcg
     1741 ctttttttct tccggcagac ggtcggcatt tacgcccgta atcgcctgca acaaggttgt
     1801 tttgccgtgg tcaacgtgtc cggcagtcgc aataatcatt tcaacaacat ctccaaaaac
     1861 cgttgctcat cttcaaggca gcgtaaatcc agccacaatc gtccgtcata aatacgacca
     1921 atcaccggca ctggcaattc acgccagcgg gcggctaatg actcaaggtg gctaccgcgt
     1981 ccatcatggg gggtaaacgt taatgccgcg ctcggcaggc gatcaaccgg cagcgaacca
     2041 ctgccgatct gcgaaagaca tggcataacc tgtaccgcaa actccgcgcc gtaatgtgcg
     2101 gcaaggagag cctgtaaacg ttgtgcctgg atttgaatga cctctgcgct gcgggtaagc
     2161 aggcgcaggg tcggtaattt ttcactcaga gcttcagggt gtaaataaag acgcaacgtg
     2221 gcttccagcg ccgcgagggt cattttatcc gcgcgtaatg cacgcttcag cgggtggctt
     2281 tgcaggcggg cgatcatctc ttttttacca acaataattc ctgcctgcgg cccgcctaac
     2341 agcttgtcgc cggagaagct caccagactg acgcccgccg caatcaactc ctgcggcatc
     2401 ggctctttcg gcaaaccgta ctggctaaga tcgaccagcg agccactgcc taaatcagtc
     2461 accactggaa catccagttc tttgccgagc gccaccagtt ccgcttcatc tatcgctttg
     2521 gtgaacccct gaatgctgta gttactggta tgtactttca tcaacagtgc ggtattttca
     2581 ttcaccgcct gacgataatc attcgcgtgc gtgcggttgg tggtccctac ttcgtgtagg
     2641 gtgcatcctg cctgacgcat cacatcggga atacgaaacg cgccgccaat ctccaccagt
     2701 tcgccgcgag aaaccaccac ctctttcccg ctggcagtgg ccgccaacat caataacacc
     2761 gccgccgcat tgttattaac gatacaggca tcttccgccc ccgtaatacg gcacagcagc
     2821 tgcgccagcg cccgatcgcg atgtccgcgt ccggcgtcgt ccagatcgta ctcgagggtc
     2881 actggcgaac gcatagcctt cgtaacggct tccaccgcgg cttccgcctg taaagctcgc
     2941 ccaaggttgg tatgcagcac ggttcccgtc aggttgatca ccggacgcag cgcgctttgc
     3001 gcctctttcg tcaaccgggc atcgacttct tgcgcccagt tttcacacca cgcgggcagc
     3061 gtctggctgt cacgaatcac ttctcgcgct tcgtcaagca tctgacgcag caattccacc
     3121 acgcgggtgt gaccataagt atcacgcaaa gaaaggaagg agctatcgcg caataagcga
     3181 tcaatagccg gaagttgact atagagggaa cgcgtttcgg ttgtcatagg aaacctggct
     3241 gatcaaggcc ctctcacacg gagaagggcg tttaacataa ctacggattg taacgtgaga
     3301 tgggtcagga ggacatatcg cgcatcaagc ctttggcggt tcggtgcgag caaaactttc
     3361 gcggctaaac aggttttcga ccagtttgac taagtgtggg cggtcgacac accagcctgg
     3421 cgcaacgcgg cggaaattga gatatccgac ggcacaggca atggcgatag tcgccagatt
     3481 gaccgtatcg gttttgagcg tgccatcgac cagatatcct tccagcacat ccagactgcg
     3541 gttgattttc tcacgctgac gtaacaattc atcttcagac tgctgcgccg ccggacgcgc
     3601 ctgttcacgc accgatacca gcccggcatc cataatgcca tccgccagcg cctcaatttt
     3661 gcgcacccgc aacgactcca gcggatcacg cggcaacatc gccggagcaa cattcattaa
     3721 ttcaatatat tcagcgatga tcggcgaatc aaaccagcat tcgccctctt cggtcaccag
     3781 caccggcact tttcctaacg ggttaaattg cgccacgccg ttgtccgcgt tatagggcag
     3841 ttcattaata aattcgaacg ttatgccctt ttctaacaac agaatagaaa gtttgcgtac
     3901 aaacgggctg gtgtagctac cgacgagttt catgccgagt cctttgtgcg aggaaaaata
     3961 tcagtatggc tcaggctcgc gcaaaacaca ggtgatacaa gtctcaatga tccaaataaa
     4021 gataatgcat ccaactggtc attcttagca gcactttccg catcaccgaa acatgactga
     4081 aatggtcgga gtaaactgcc acctgggcgt agatgccgtc gggtaaggca tcgatatcag
     4141 cgttagggtc cagttcaatg gttcccagca caccgtccgt gccgggcacg accgttaatg
     4201 attgcaatac cccttgcgcc tgatacgaac cgcctggtac gacaggtaaa atactggtca
     4261 gtttgccgtg gaacacctgc ccaggtagcg cgttaaacac cacttccgca tcatcgccag
     4321 gtttcagacg taacagcgag ttttgccgaa attgggcgac aatttgccgt ttttgctcgg
     4381 ggatgaagac catcaccgga cgcagcggca aggcagcggc gtatgtacct gggcggatca
     4441 gtacctgagt gacgtagcca ttgctcggtg cgcgaatgac ggtctgctca aggttatatt
     4501 ttgcttcagt aagttgcgct cttaagctca caatctgcga ttgctcgccg ttaaccatac
     4561 tgtcgagctg gctctggatc tgcgcctgct ccgccaccga gcctttcacc agcgcatcct
     4621 gcgcgaggaa attttgccgc gcatcgtcga tgtcacgttc cgagaacgga ttcaccgccg
     4681 cctggctgcc tttcagataa cgttgataat ttttaaacag acggtcgcgc tccgctgaaa
     4741 cctgggtggt gttggcctgc gcttcagtga gttgcgcgcg cagcgtcttt atattatgcg
     4801 tcgccgtcat caggtctgcc tgaagtctgt caactcgcgc ctggtaacga accgggtcga
     4861 gcttaaaaag cacctcgccc ttctgaataa gctgattatt tttgtcggtg acttcagtaa
     4921 caattcccgt cacctgtggc gtgatgggga tcgctatcac tgccttttgc gcggtaaaag
     4981 tgtaagggtg gttgtagttc atcaacaaaa tcaaaccact caccagaaac acgcctccca
     5041 gcgccgccgt cgccagcgtc cactgattca ccggaatgcg gaagatttta aagaccgccc
     5101 acgccagcgc cacgtaagtt aaaacaatca atagatccat aattagatct ccggaaacgt
     5161 ggaatagtca gtctttttct cggcggcgag ttggtgctcc agccgggaaa ttcggtcaga
     5221 aagtgcggcg atttccgggt cagtcgcttt ctcttgcgac gcaacatgtg actgcatgcc
     5281 ccagccgcgc tccggttgat agagcgtcgc ccagatccac aaaaacggcc agataacatg
     5341 cagagtaaac aggctcaccc agcccgccgt atgaatagcg tcggcatggg gatggttgcg
     5401 ctttttggca atcagataag ggatgtcatg tatcgcgatg attccataaa aaatcaccag
     5461 aaagacgaag atcagcactc ccaacgcgaa atagtttaga aacatatctg cctcggattc
     5521 acgtttatca gtgttgtttt ttggctggca gccagaaggg agtcaggctg atattttgac
     5581 aataatccgg gttcgcgatt ctcgccataa caccaaagaa taattttaag aggtgatgag
     5641 ttgcttagtt acataacgat ttcatgacga aggcataaca tgctgtagat cacatcaggt
     5701 gaacgccgta agaaaatatc ttgtgattca gatcacaaag attcaacaaa ccatcaaaac
     5761 aaaaatgtga cattactcac atttaaatgc catttttagc gaaaatcgcc gccctgttgc
     5821 ttttttacac aagcattttg tgatcagcat cacgtcaatt acctctctac cccctacatt
     5881 tatgtgactg atatcacaca aaaggccgcc gactggacag ttaaccgatt cagtgccaga
     5941 tttcgcagta tctacaaggt ccggctacct ctgccgccac attaacaaaa aacctcgggc
     6001 ttccagcctg cgcgacagca aacataagaa ggggtgtttt tatgtcatcc gatattaaga
     6061 tcaaagtgca aagctttggt cgtttcctca gcaacatggt gatgccaaat atcggcgcgt
     6121 ttatcgcgtg gggtatcatc accgcgttat ttattccaac agggtggtta ccgaacgaaa
     6181 cgctggcgaa gctggtcggg ccgatgatca cttatctcct gccgctgctg atcggttata
     6241 ccggtggtaa gctggttggc ggcgaacgtg gcggcgtagt cggtgccatt accaccatgg
     6301 gcgttatcgt cggcgcagac atgccgatgt tcctcggctc tatgattgca ggtccgctgg
     6361 gcggctggtg cattaagcac ttcgaccgct gggtagacgg taagatcaaa tccggttttg
     6421 agatgctggt gaataacttc tccgcaggca tcatcgggat gatcctcgct attctggcat
     6481 tcctcggcat tggcccgatt gttgaagccc tgtccaaaat gctggcggcg ggcgttaact
     6541 tcatggttgt ccatgacatg ctgccgctgg cgtctatctt tgttgaaccg gcgaaaatcc
     6601 tgttcctcaa caacgccatt aaccacggta tcttctcgcc gctgggtatt cagcagtccc
     6661 atgaactggg taaatccatc ttcttcctga ttgaagctaa cccaggtcca ggtatgggcg
     6721 tgctgctggc gtacatgttc tttggtcgtg gtagcgctaa acagtctgcg ggcggtgcgg
     6781 caatcatcca cttcctgggg ggtatccacg aaatctactt cccgtatgtg ctgatgaatc
     6841 cgcgtctgat tctggccgtt atcctcggcg gtatgactgg cgtattcacg ctgactatcc
     6901 tgggcggtgg gctggtttct ccggcatctc cgggttctat ccttgctgta ctggcgatga
     6961 cgccaaaagg tgcttacttc gctaacatcg cgggtgtgtg tgcggcgatg gcagtttcct
     7021 tcgttgtctc tgccattctg ctgaaaacca gcaaagtgaa agaagaagat gatattgaag
     7081 ctgctgcacg tcgcgttcag gaaatgaaag ctgagtctaa aggcgcatct ccgctgtctg
     7141 cgggcgatgt gactaacgac ctgagccacg tccgtaaaat catcgttgcc tgtgatgccg
     7201 gtatgggttc cagtgcgatg ggtgcaggcg tgctgcgtaa gaaaattcag gatgcaggtc
     7261 tgtcgcagat ttcggtcact aacagcgcga tcaacaacct gccgccagat gtggacctgg
     7321 tcatcactca ccgtgacctg accgaacgcg ctatgcgcca ggttccgcag gcacagcata
     7381 tttcgctgac caacttcctc gacagcggcc tgtacaccag cctgaccgaa cgtctggttg
     7441 ctgcccagcg ccatactgaa aacgaagtga aagtgaaaga cagtctgaaa gacagctttg
     7501 acgattccag tgctaacctg ttcaaactgg gcgcggagaa catcttcctc ggtcgcaaag
     7561 cggccaccaa agaagaagcg attcgttttg ctggcgagca gctggtgaaa ggcggttacg
     7621 ttgagccgga atacgttcag gcgatgttgg atcgtgaaaa actgaccccg acttatctgg
     7681 gtgagtctat cgcggtgcca cacggtacgg ttgaagcgaa agatcgcgtg ctgaaaacgg
     7741 gcgtggtgtt ctgccagtac ccggaaggcg tgcgcttcgg tgaagaagaa gatgacattg
     7801 cccgtctggt gattggtatt gctgcccgta acaacgagca cattcaggtg atcaccagcc
     7861 tgaccaacgc actggatgat gagtcagtta tcgagcgtct ggcgcacacc accagtgtgg
     7921 atgaagtgct ggaactgctg gcaggtcgta agtaatccat acccaccctc tccgcatgaa
     7981 gaaggtgggg ctaattgctt gatgcgcttc gcttatcagg cctacaacat aaagcacaat
     8041 ttgttttgtc ggtcggataa ggtgtttacg ccgcatccgg cagtcggtgc acaatgcctg
     8101 atgcgacgct gacgcgtctt atcagaccta caggtcagtg ccaaacacgt aggccggata
     8161 aggcggttta cgccgcatcc ggcaaaaagc ccctctcctc acggagaggg tttgggtgag
     8221 ggaaaagcct caccccagcc ctctcgggta aaaacattga tgaaggttaa tactatgaaa
     8281 gcattacatt ttggcgcagg taatatcggt cgtggcttta tcggtaaact gctggcagac
     8341 gcaggtatcc aactgacgtt tgccgatgta aatcaggtgg tgcttgatgc cctgaatgcc
     8401 cgtcatagct atcaggttca tgtggttggt gaaaccgagc aggtagatac cgtttctggc
     8461 gtcgatgccg tcagcagcat tggtgatgat gtcgttgatc tgattgctca ggttgattta
     8521 gtcactaccg ccgttggccc ggtcgtgctg gaacgtattg ctccggccat cgccaaaggg
     8581 ctggtgaaac gtaaagaaca aggtaatgaa tctccgctga acatcatcgc ctgtgaaaac
     8641 atggtccgcg gcaccacgca gctgaaaggc catgtgatga acgccctgcc cgaagacgcc
     8701 aaagcgtggg tagaagaaca cgttggcttt gttgattccg ccgttgaccg catcgtaccg
     8761 ccttcggctt cggcaactaa cgatccgctg gaagtgacgg tagaaacctt cagcgaatgg
     8821 attgtcgata aaacccagtt caaaggcacg ctgccaaaca tccctggcat ggaattaacc
     8881 gacaatctga tggcatttgt cgaacgtaaa ctcttcaccc tgaatacagg tcatgctata
     8941 accgcgtacc tcggaaaact ggcgggtcat cagaccattc gtgacgcaat tctcgacgag
     9001 aaaatccgcg cggtggtaaa aggggcaatg gaagaaagtg gtgcagtatt gatcaagcgc
     9061 tacggctttg acgctgacaa gcatgcggca tacatccaga aaatccttgg tcgtttcgag
     9121 aacccgtacc tgaaagatga tgtagaacgc gtaggccgtc agccgctgcg taaactgagt
     9181 gctggcgacc gtctgatcaa gccactgctc ggaaccctgg aatatggtct gccacataaa
     9241 aacctgattg aaggtattgc cgctgcaatg cacttccgca gtgaagatga tccgcaggct
     9301 caggaactgg cagcgctgat tgccgacaaa ggcccacaag ccgcgctggc acagatttcc
     9361 ggtcttgatg ccaacagcga ggttgtatcc gaggcggtaa ccgcttataa agcaatgcaa
     9421 taatggtgga ccaggcgcag gataccctgc gcccgaataa cagattgtca gatatgcagg
     9481 caacaatgga acaaacccag gcctttgaaa accgtgtgct tgagcgtctg aatgctggca
     9541 aaaccgtgcg aagctttctg atcaccgccg tcgagctttt gaccgaggcg gtaaatcttc
     9601 tggtgcttca ggtattccgc aaagacgatt acgcggtgaa gtatgctgta gaaccgttac
     9661 tcgacggcga tggtccgctg ggcgatcttt ctgtgcgttt aaaactcatt tacgggttgg
     9721 gcgtcattaa ccgccaggaa tacgaagatg cggaactgct gatggcattg cgtgaagagc
     9781 taaatcacga cggcaacgag tacgctttta ccgacgacga aatccttgga ccctttggtg
     9841 aactgcattg cgtggcggcg ttaccaccgc cgccacagtt tgaacctgca gactccagtt
     9901 tgtatgcaat gcaaattcag cgttaccaac aggctgtacg atcaacaatg gtcctttcac
     9961 tgacagagct gatttccaaa atcagcttaa aaaaagcctt tcaaaagtaa gtactttcgc
    10021 ttacttctcc tctacctgtt tcggacgata aagctgacga taatactcca gacgttgtag
    10081 atataacggc acgctttcat caggtattcc ggacggaatc gcgttacggg gaggtagttt
    10141 tttcagatac tcccggaacg cctggctcga tgccatgaaa tctacggctt tatcgataag
    10201 aagcggaacg ttttcaccta atttgcccat gatgatatct ccgtattacc ccgcgccggg
    10261 aatgcgcggc cgccaatttt agtttagatc ccggtataaa tgacattaag gaaaacgtgc
    10321 tcaatcctca ataatcacct gcttctgttg actatctttt ttctttaaat ttcttaacta
    10381 atcaatgtat tgggtgattt tttaacgcat taatggttac aatctggaac tattacaatg
    10441 ccatcagttg tgaacaaagc acctggtcgc gcatactaga ggctataaat ttatctctat
    10501 cagaagtcat cacatgaaag aagtcgaaaa aaacgaaatc aaacgtctca gcgatcgtct
    10561 ggacgccatc cgtcaccagc aggccgatct atcgctggtt gaagccgcag acaaatatgc
    10621 cgaactggaa aaagagaaag ccacgctgga agcagaaatt gctcgcctgc gcgaagttca
    10681 cagccagaaa ctcagcaaag aagcgcagaa actgatgaag atgccgttcc agcgtgcaat
    10741 caccaaaaaa gagcaggctg atatgggcaa actgaaaaaa agtgttcgcg gactggtggt
    10801 tgtgcaccca atgaccgcgc tgggccgtga aatgggcctg caagagatga cagggttctc
    10861 aaagactgcg ttttaagttc ctttctcgtc gctgtaacta ctgccactca cgatttactc
    10921 gctcctgctg gcacatgcag gagcctgtgc cactcattaa atacgatgtc tttcttaccg
    10981 taaactgttt cagtttcccc tcgagtttga ggtatttttg caggctaaaa tctgaaaatt
    11041 tgttgcgcaa caacaagtga tatataaatc ataaaaataa ataatcgaat tatgatgcat
    11101 gattatttat ctttccccat aaacacagca aatttcatca caacacatca tttatatgaa
    11161 tataaaatct ccctgcgctc cccagcctaa gacactaaac atttgacatt gattgatacc
    11221 gctcattaaa attcgcgcga ctcataacat tcgctacaaa ctgaaatcag ttgtcattta
    11281 ataaaacgtc cgtaatacca attatttcaa taaatctctt ataagtttta ttgcgaatac
    11341 atatataaaa aacattcatc agaagaatga atatggcgta attatcaaca tattggggtt
    11401 atgaaaagga attcatccta tgagcatgaa aacactttta aacgtgttaa caatattttc
    11461 actaggtaca ttaacaggct gtgattatat tgaaaaagcg agtctgatcg atgatcttgc
    11521 caaacagcaa gagttgcaaa aaagtaaaat agaggagctt gagaaacaac aagaacaata
    11581 taaaaataaa gcagaactgt ttgaaaaaca acaaaataat atcacaaaca gcaccagaat
    11641 acttgccagc attgtaaaga cagtcaaaga aaaacaggac gaatttgtct tcaccgaatt
    11701 taacccgcta caaacccaat acttcatttt aaataacggc tcggtcggtt tggcaggccg
    11761 ggtgctggct attgatgcgg ttgaaaatgg cagtgtcatt cgtatttcgc tggttaactt
    11821 attaagcgtt cccgtatcaa atattggctt tcatgcgaca tgggggaatg agaaacccac
    11881 cgatgccaaa gctctggcga aatggcaaca attactattc aacaccaccc tgaattccac
    11941 tctgcaatta atgcctggtc aatggcaaga cattaatctg acattaaaag gcgtctcgcc
    12001 taataacctg aagtatctga agttgtccat caatatggct aatcttcagt tcgacactgt
    12061 tcaacctgct gaaacccggc agcggaaaaa caaaaaataa atgcagcttc tgcgcttata
    12121 tcaaggaata gagagcatca ataatgaaca aaatatttaa agttatctgg aatccggcga
    12181 caggcagtta caacgtcgcc agcgaaacgg cgaagagccg tggcaaaaag agcgggcgca
    12241 gtaagctgtt aatttctgcc ttggttgcag gtgggctgtt atcgtcattt ggggcactgg
    12301 ctgataacta cgaggggcag ccagtggatt atggcaatgg ctcagcaggt gacggctggg
    12361 ttgctatcgg taaaaattca aaagcaaata cctttgtcaa tggctctggt gcgagcactg
    12421 cgctaggtta tgacgcgata gctgaaggtg agtacagctc agctataggc tcacgaactc
    12481 atgcgattgg tggtgcatca atggcctttg gggttagtgc acaagcaata ggtgacagaa
    12541 gtgtcgcgct gggtgcatct tcagtagcaa ctggcggtcg ttcgatggct cttggtcgtt
    12601 acgcaaagac gaatggtttt acatctcttg ctattgggga caccgccctt gccgatggtg
    12661 aaaaaactat tgcgttagga aatacggctc aagcttacga aattatgagc atcgccctcg
    12721 gtgatcatgc taatgcctca aaagaatatt caatggcgct gggagcaagt agcgttgcat
    12781 ctgcggctaa cgccattgcc gtaggccgcg acagtgctgc taacggcaca gacagccttg
    12841 ccttaggcag actatctctc gccagcgcag caaacgcgat tgctatgggt gcagagagcg
    12901 aagctgctga aaatgcgacg gcgattggct ttaacgcgga tgcgatcggg aagtccagcc
    12961 tggcgctggg agacaacgcc agcgctggtg aaaccaactc catcgctata ggtcaaggca
    13021 gcgaggcaag caaacttgat tcaattgcgc tgggaagtaa cagcaggtca gcgggtgaga
    13081 acgccattgc actgggcaac aacagtaatg cggggggcaa aaacagcctt gctttcggtt
    13141 ttaacaccac cgcaaatggt gataacgcag tcgccattgg tgcaaattct tccgcaggtg
    13201 ctgacaatac cgtgtctgta ggtagctcta gtttaaagcg caaaattgtc aatatgggta
    13261 atggtgacat caataatgtc agctccgatg ccatcaatgg atcacaactt tatgccatca
    13321 gtaagtctgt atccgacagg cttggaggat atcatgatga tcctgataat gttataaact
    13381 ctgacgggac gcttaaagct cccacatatt atctacaaag tggccaatac aataacgtgg
    13441 gagaggctct ccagagcatc gacaataaca cgttgcactg ggacagcaaa agtaataaat
    13501 acagtgccag ccatactgtc tttaatgcaa atggctcagt taaaagcacc tcggcgaaaa
    13561 acatcattac cgacgtcgct gatggcacga tttcagccac cagttccgac gccgttaacg
    13621 gttcgcagtt gtataatctg aaacaagatg ccttgctatg ggatggcact gcgtttagcg
    13681 ccaaacatgg caccagcaat actaacagca aaatcaccaa cgttgcggat ggtgctgttt
    13741 cagcctccag taaagatgcc gttaacggtt cgcagttgta tgatctgaaa caagatgccc
    13801 tgctatggga tggcactgcg tttagcgcca aacatggcac cagcaatact gacagcaaaa
    13861 tcaccaacgt actggcaggc acagtatctt ccaccagcac cgatgccatt aatggtacac
    13921 aacttcaaag cactgcaagc accattacgt cctatttggg cggcggcgcg gcaatgagta
    13981 atgacggcgt atttaccggt cctcaatata atattggtgg taagaattac accaatgttg
    14041 gcgcagctct taccgctctg gacaatgacg ccctgttgtg ggatgcaacc gctaattcaa
    14101 atactggcgc attcagtgct gcacacggaa cactcaaaac cgccagcaag attaccaacg
    14161 ttgcaaatgg tgacatatta gccaacagtt atgatgctat taatggctca caactttata
    14221 gtttagctga ttcatttacg tcataccttg gcggtggtgc tgatatcaac aatgcgggtg
    14281 ttctcatagc cccgacctat actatcggtt cgaaaacgta caacaacgta ggtgatgccc
    14341 ttaacgcgat taactcatca ttcagcacct caatcagtga tgccctgctg tgggattcta
    14401 cagcgaacaa attcagcgcc aaacacggtg gtaccaatag cattattact aacgttgcag
    14461 acggtacaat ttcctctacc agcagcgatg ctgtaaatgg ctcgcaactc tatgacacga
    14521 gcaagtacat tgctgatgcc ctgggtggtg acgcagaagt taacgctgac ggcaccatga
    14581 ctgcaccgac ttactctatc gctaatgaag attaccacag cgtaggtgaa gccctggatg
    14641 cgctcgatga taacgcgctg ctgtgggatg aagacgcagg tgcctacaac gccagccatg
    14701 atggcaatgc cagcaaaatt accaacgttg cggcaggtga tctctccaca accagtaccg
    14761 atgctgttaa cggttcccag ttaaacgcaa ccaatattct ggttacgcaa aatagccaaa
    14821 tgattaacca gcttgccggt aacaccagcg aaacctacat cgaggaaaac ggtgcgggta
    14881 ttaactatgt acgtaccaac gacaacggct tagcgttcaa cgatgccagc gcctcaggta
    14941 ttggtgctac ggctgtgggt tataactctg ttgccgaagg tgatagcagc gtggccattg
    15001 gtcaaggtag cagcagctcc gttgataccg gcattgcctt gggtagcagc tccgtttcca
    15061 gtcgcgtgat tgttaaaggt tctcgtaaca ctagtgtaac ggaaaatggc gtagttatcg
    15121 gttatgacac cactgacggt gagttgctcg gtgcattgtc aattggtgat gacggtaaat
    15181 accgtcaaat catcaacgtc gccgatggtt ccgaagccca tgacgccgtt acggttcgcc
    15241 agctgcaaaa tgccattggt gcagtcgcta ccacgccgac taaatacttc cacgctaact
    15301 caacggctga agactcactg gcagtcggtg aagactcgct ggcaatgggc gcgaaaacca
    15361 tcgttaatgg taatgcgggt attggtatcg gcctgaacac gctggttctg gctgatgcga
    15421 tcaacggtat tgctatcggt tctaacgcac gcgcaaatca tgccgacagc attgcaatgg
    15481 gtaatggttc tcagactacc cgtggtgcgc agaccaacta cactgcctac aacatggacg
    15541 cgccgcagaa ctccgtgggt gagttctctg tcggcagtga agacggtcaa cgtcagatca
    15601 ccaacgtcgc agcggggtcg gcggataccg atgcggtcaa cgtgggtcaa ttgaaagtaa
    15661 cggacgcgca ggtttcccag aatacccaga gcattactaa cctgaacact caggtcacta
    15721 atctggatac tcgcgtgacc aatatcgaaa acggcattgg cgatatcgta accaccggta
    15781 gcactaagta cttcaagacc aacaccgatg gcgcagatgc caacgcgcag ggtaaagaca
    15841 gtgttgcgat tggttctggt tccattgctg ccgctgacaa cagcgtcgcg ctgggcacgg
    15901 gttccgtagc agacgaagaa aatacgatct ctgtgggttc ttctaccaac cagcgccgta
    15961 tcaccaacgt tgctgccggt gttaatgcca ccgatgcggt taacgtttcg caactgaagt
    16021 cttctgaagc aggcggcgtt cgctacgaca ccaaagctga tggctctatc gactacagca
    16081 acatcactct cggtggtggt aacggcggta cgactcgcat cagcaacgtc tccgctggcg
    16141 tcaacaacaa cgacgcagtg aactatgcgc aactgaagca aagcgtgcag gaaacgaagc
    16201 aatacaccga tcagcgcatg gttgagatgg ataacaaact gtccaaaacc gaaagcaagt
    16261 tgagcggtgg tatcgcttct gcaatggcga tgaccggtct gccgcaggct tacacgccgg
    16321 gtgccagcat ggcctctatt ggtggcggta cttacaacgg tgaatcggct gttgctttag
    16381 gtgtgtcgat ggtgagcgcc aatggtcgtt gggtctacaa attacaaggt agtaccaata
    16441 gccagggtga atactccgcc gcactcggtg ccggtattca gtggtaatga tccattaacg
    16501 aatgggtaaa cgccacattg cctgatgcgc tacgcttatc aggcctacgg ggtgattcag
    16561 cacattcctg taggtcggat aaggcgttca cgccgcatcc ggcaagataa ggcactccgg
    16621 gtcaacaatc taagggcaat tctctgatga ggattgccct tttctttacc agacatctcc
    16681 caccacaaga attggcccta ccaattcttc gcttatctga cctctggttc acaattcccc
    16741 aattaaaact cacatcaatg ttgccaacac gtaacattta gttaaccatt cattgtcatt
    16801 acccctacac aacacaattg gcagtgccac ttttacacaa cgtgtgacaa agagatgggc
    16861 aacagactca ttacacggcg tgtgtggact gcaggagacc tgcaatgaat ctctggcaac
    16921 aaaactacga tcccgccggg aatatctggc tttccagcct gatagcatcg cttcccatcc
    16981 tgtttttctt ctttgcgctg attaagctca aactgaaagg atacgtcgcc gcctcatgga
    17041 cggtggcaat cgcccttgcc gtggctttgc tgttctataa aatgccggtc gctaacgcgc
    17101 tggcctcggt ggtttatggc ttcttctacg ggttgtggcc catcgcgtgg atcattattg
    17161 cagcggtgtt cgtctataag atctcggtga aaaccgggca gtttgacatc atccgctcgt
    17221 ctattctttc gataacccct gaccagcgcc tgcaaatgct gatcgtcggt ttctgtttcg
    17281 gcgctttcct tgaaggagcc gcaggctttg gcgcaccggt agcaattacc gccgcattgc
    17341 tggtcggcct gggctttaaa ccgctgtacg ccgccgggct gtgcctgatt gttaacaccg
    17401 cgccagtggc atttggtgcg atgggtattc cgattctggt cgccgggcag gtaacaggca
    17461 tcgacagctt tgagattggt cagatggtgg ggcgtcagct gccgtttatg accattatcg
    17521 tgctgttctg gatcatggcg attatggacg gctggcgcgg tatcaaagaa acctggcctg
    17581 cggtcgtggt tgcgggcggg tcgtttgcca tcgctcaata cctcagctct aactttattg
    17641 ggccggaact gccagacatt atctcttcgc tggtatcgct gctctgtctg acactgttcc
    17701 tcaaacgctg gcaacccgtg cgcgtattcc gcttcggtga cttaggggcg tcacaggttg
    17761 atatgacgct ggctcacaca ggttacacgg caggtcaggt gctgcgcgcc tggacaccgt
    17821 tcctgttcct gaccgccacc gtaacactgt ggagtatccc gccgtttaaa gccctgttca
    17881 cttcaggcgg tgcgctgtat gagtgggtga tcaacattcc ggtgccgtac ctcgataaac
    17941 tggttgcccg tatgccgcca gtggtcagcg aagcgacagc gtatgccgcc gtgtttaagt
    18001 tcgactggtt ctctgccacc ggtaccgcca ttctgtttgc tgccctgctc tcgattgtct
    18061 ggctgaagat gaaaccatct gacgctatca gcaccttcgg cagcacgctg aaagaactgg
    18121 ctctgcctat ctactccatc ggcatggtgc tggcgttcgc ctttatctcg aactattccg
    18181 gtttgtcatc aacactggcg ctggcactgg cgcacaccgg tcatgcattc accttcttct
    18241 cgccgttcct cggctggctt ggggtattcc tgaccgggtc ggatacgtca tctaatgctt
    18301 tgtttgccgc tttgcaggct accgccgcac aacaaattgg cgtttctgac ctgttgctgg
    18361 ttgctgccaa caccaccggt ggtgtcaccg gcaaaatgat ctctccgcaa tctatcgcta
    18421 tcgcctgtgc ggcggtaggc ctggtgggca aagagtctga tttgttccgc tttactgtca
    18481 aacacagcct gatcttcacc tgtatggtgg gcgtgatcac cacgcttcag gcctatgtct
    18541 taacgtggat gattccttaa tgattgtttt acccagacgc ctgtcagacg aggttgccga
    18601 tcgtgtgcgg gcgctgattg atgaaaaaaa cctggaagcg ggcatgaagt tgcccgctga
    18661 acgtcaactg gcgatgcaac tcggcgtgtc gagaaattca ctgcgcgagg cgctggcgaa
    18721 actggtaagc gaaggtgtgc tgctcagtcg acgtggcggc gggacgttta ttcgctggcg
    18781 tcatgacaca tggtcggagc aaaacatcgt ccagccgctg aaaacgctga tggccgatga
    18841 tccggattac agtttcgata ttctggaagc ccgctacgcc attgaagcca gcaccgcatg
    18901 gcatgcagca atgcgcgcca cacctggcga aaaagaaaag attcagcttt gctttgaagc
    18961 aacgctaagt gaagacccgg atctcgcctc acaagcggac gttcgttttc atctggcgat
    19021 tgccgaagct tcacataaca ttgtgttgct gcaaaccatg cgcggtttct tcgatgtcct
    19081 gcaatcctct gtgaagcata gccgccagcg gatgtacctg gtacccccgg ttttttctca
    19141 gctgaccgaa caacatcagg cggtcattga cgccattttt gtcggtgatg ctgacggggc
    19201 gcgtaaagca atgatggcgc accttagctt tgttcacacc accatgaaac gattcgatga
    19261 agatcaggct cgccatgcac gaattacccg cctgcctggt gaccataatg agcattcgag
    19321 ggagaaaaac gcatgattat ttccgcagcc agcgattatc gcgccgcagc gcaacgcatt
    19381 ctgccgccgt tcctgtttca ctatatggat gggggggcat attctgaata caccctacgc
    19441 cgcaacgtgg aagatttgtc agaagtggcg ctgcgccagc gtattctgaa aaacatgtct
    19501 gacttaagcc tggaaacgac gctgtttaat gagaaattgt cgatgccggt ggcgctaggt
    19561 ccggtaggtt tgtgtggcat gtatgcgcga cgcggcgaag ttcaggctgc caaagccgcg
    19621 gatgcgcatg gcattccgtt tactctctcg acggtttccg tttgcccgat tgaagaagtc
    19681 gctccagcta tcaaacgtcc gatgtggttc cagctttatg tgctgcgcga tcgcggcttt
    19741 atgcgtaacg cactggagcg agcaaaagcc gcgggttgtt cgacgctggt tttcaccgtg
    19801 gatatgccga caccgggagc gcgttatcgt gatgcgcatt ctgggatgag cggcccgaac
    19861 gcggcaatgc gccgctattt gcaggcggtg acgcatccgc aatgggcgtg ggatgtgggc
    19921 ctgaacggtc gtccgcatga tttaggtaat atctcggctt acctcggcaa accgaccgga
    19981 ctggaagatt acatcggctg gctggggaat aacttcgatc cgtccatctc atggaaagac
    20041 cttgagtgga tccgcgattt ctgggatggc ccgatggtga tcaaagggat cctcgaccca
    20101 gaagatgcgc gcgatgcagt acgttttggt gctgatggaa ttgtggtttc taaccacggt
    20161 ggccgccagc tggacggtgt gctctcttct gcccgtgcac tgcctgctat tgcagatgcc
    20221 gtgaaaggtg atatcgccat tctggcagat agcggaatac gtaacgggct tgatgtcgtg
    20281 cgtatgattg cgctcggtgc cgacaccgta ctgctgggtc gtgctttcct gtatgcgctg
    20341 gcaacagcgg gccaggcggg tgtagctaac ctgctaaatc tgatcgaaaa agagatgaaa
    20401 gtggcaatga cgctgactgg cgcgaaatcg attagcgaaa ttacgcaaga ttcgctggtg
    20461 caggggctgg gtaaagagtt gccggcggca ttggctccga tggcgaaagg gaatgcagct
    20521 taagggttag acgaatatct gctatcctgc ccccgcacta agggggcagt atgctaaaca
    20581 tcgtacttta cgaaccagaa attccgccaa atactggcaa catcatccgt ctttgcgcta
    20641 ataccggctt tcgtctgcat atcatcgaac cgatgggatt tgcctgggac gataagcgcc
    20701 tgcgccgcgc ggggctggac tatcacgagt ttaccgccgt tacgcgtcat catgactatc
    20761 gcgcgtttct cgaagcagaa aatccccagc gtctgtttgc cctcaccacg aaaggtacgc
    20821 ctgctcacag cgccgtaagc tatcaggatg gcgattatct gatgttcggc ccggaaacac
    20881 gcggcctgcc agcgagcatt cttgatgccc tgcccgctga acaaaaaatt cgtattccaa
    20941 tggtgccgga cagccgcagc atgaacctgt ccaatgcggt gtcggtcgtg gtgtatgaag
    21001 cctggcggca gttggggtat ccgggagcgg tattgagaga ttagttactg taggccggat
    21061 agatgattac atcgcatccg gcacgatcac aggacatcag atcccatcac catactcaaa
    21121 cgtatggtta atgccgttga aatgctgatc catatccatt gacggtttat cgctatccgg
    21181 cttgccgaca atgcgtgccg gaacgccagc ggcagtagtg tgcggcggca ccggttgcag
    21241 caccacggaa cctgcgccaa tcttcgcgcc gcgcccaact tcaatattgc cgagaatttt
    21301 cgcgcccgcg ccaatcatca caccttcacg aattttcggg tgacggtcgc caccagattt
    21361 acccgtaccg ccaagcgtca cagattgcag aatcgatacg tcgttttcaa tcaccgccgt
    21421 ttcaccaacg acgatgcctg tcgcgtggtc gagcatgata ccgcgaccaa tttttgctgc
    21481 cgggtgaata tcaacctgga acgtcacaga aacctggttt tgcagaaaaa tagccagtgc
    21541 gcgacgcccc tgattccaca accagtgacc gatgcgatac gcctgcaaag catgaaaacc
    21601 cttcaggtat agcaacgggg ttgagtattt atccaccgcc gggtcacggg tacgcaccgc
    21661 ctgaatatca caggccgcag aggcgatcat ttccgggtcg gcggcgtagg cttcttccac
    21721 cacttcacgg atagcaatag ccggcataat gggcgatgac agcttgttcg ccagcatgta
    21781 gctcagtgca ctgccaaggt tttcgtgctt gagtagcgtc gcgtggtaaa aactggccag
    21841 cattggctca cagtccgcca gcgttctggc ttcagcttta atattgttcc agacaatttc
    21901 cagttcttca cacgacattg cttactccac acgatgagat aatgaccgac ccgttctgcg
    21961 cgggccgggt catagtggta acaaaggttc cctgggttta gtggctgctg cgctcgtcct
    22021 tgcgtgcacg acctagtaaa gtcaatgctg cctcgcgcgc gttttttccg caatataata
    22081 cttgataaat ttcctcggtt attggcattt caacgccgaa gcgatgcgcc agttcgcgga
    22141 cttctttcgt attgcggtag ccttccacca cttgaccaat tttctcctgc gcgctttgta
    22201 catccatgcc ctgaccgagc atcatgccaa aacggcggtt acgcgactgg ttgtcggtac
    22261 aggtaagcac cagatcgccc agccccgcca tgcccataaa ggtggcagga tcggcaccca
    22321 gcgccgcgcc aagacgcgac atctcagcca gcccacgagt gatcagcgcc gtacgcgcat
    22381 tcgcaccaaa accgataccg tcggacatcc ccgcgccaat ggcaatgacg tttttcaccg
    22441 cgccgccaag ctgcacaccg atgaaatccg ggttgctgta aacgcggaaa cttttgccac
    22501 agtgcagcaa ttgctggagg tcatcggcaa aggtctggtc cgtcgaggcc agcgaaatgg
    22561 ccgtcggtaa acctgccgcc agttctttcg caaacgttgg gccagagata accgccagcg
    22621 gaatttgatc gcctaaggcc tcacgcgcca cgtcctgtaa cagacgtccg gtttctgctt
    22681 ccagcccttt ggttgcccac accagacgcg catcaggacg catcagcggt ttaatctggc
    22741 gcagcacttc accaaagaca tggctgggta cgacgacgag aatattacgg ctggctgcca
    22801 gcgcagtggc gagatcgctt tcaagatgga gcgtatcggg aaaaggcaca tcggggagaa
    22861 acgcggcgtt acagcggtcg cgttcaagcg ttgcgatatg ttcagggtca tggccccaga
    22921 ggacaacctc gtggccattt cttgccaggg tgatggcaag agcggtgccg tacgagccgg
    22981 caccgatcac agtcattgaa gcattacgtt ggttcatcag gcatcctgat gttcttcagt
    23041 accttcgcca gcctgctgct gcaaatagtt catgaacagc gcatcgaagt taaccggcgc
    23101 aaggttcagt tgcgggaatg taccgcggga taccatgctg gtgatgcact cacgagcata
    23161 cgggaacaga atgttcgggc agtatgcgcc caggcaatgt gccatctggg tgccttcgat
    23221 acccgcgatg gagaaaatac cgccctgctg aacttcacac aggaacgcgg tttcttcgcc
    23281 cagggaggcc gttacggtaa cacgcagtac cacttcgtaa acgtcatctg ccagttgagt
    23341 agatgccgta tccagatcaa gtttaacttc tggttgccaa tctttctgga aaacgtgcgg
    23401 cgcgttcggc gcttcgaaag aaatatcctt ggtataaata cgttggatct ggaaagtcat
    23461 ttcggtgttg ttttgttctg acatgtgtag aaaaccctta agtgttgtcc ttaaaatact
    23521 gcgcaatgta atgccaacgt tcacacgtta tttcagcagg ggatccagtc caccacgtgc
    23581 atccaatgca tacaagtcat cacagccacc aatgtgctgt gcgtcaataa aaatctgtgg
    23641 aaccgtggtg cgaccgctgc gtttgatcat ctcttcacgc ttagcggcat tgccatcgat
    23701 cggcaactcc tgaaaactca cgcccttgct gcttagcagt gcttttgcac gatggcaata
    23761 cgggcaggtt tctttggtat agatttcaac attggccata aattagctcc tgttttttac
    23821 cctgaaattt catgttgcag ggcggcagca aaatggagtg tccccagaag cttatatcaa
    23881 taagtcgctg ggggcctaat tgcagctaac ggcctgcatc atgaaagacg acaggtaaat
    23941 tatttgccgc gcaccaaagg caagttttcg ccagcccagc cagcgacgcc ttctttcagt
    24001 acgaatactt gtgcaaaacc agctttcgtc agcgcgtttg caggctcctg gcactgcatg
    24061 ccagaaccat ctaccacgat aaccggtttg tctttgtgct tctcaagctc accaacattg
    24121 ttggctttga tttcgctcgg caacaggttg atagaacctg cgatatggcc tttacggaag
    24181 tcatcacgct gacgtaaatc cacaaccaca gcgtcttctt tgttgatcag acgcgtagct
    24241 tcaccacgag taatcacctt cactttcgag gtcaggctct taaacgtagt cacaagaacc
    24301 gccaccagta acgcgatcca ggcgatactc agtatgggat gacggccaac aaattgcata
    24361 atttcttgca tggggggtaa caactcccga cgtagtgatt aaaaaaccag gaaaggagta
    24421 tacctgcgca gtgcggcaaa tacagccagc gcgttaactg gaatgcaatt ttgcggggcg
    24481 ctacgaaaaa aaagcgtaaa tctggcatct gcttgccgcg agcggtcgta tattttgatc
    24541 ttcagaggct attttatcga ttcagctgta gtaaaattac gcaaattttg actcttgagt
    24601 atgaggttgt cgcaatgtcg gtttctaaaa aacctatggt actggtgatt ctggatggct
    24661 atggctatcg cgaagaacag caggataacg ccatttttag tgctaaaacc ccggtaatgg
    24721 atgcactgtg ggccaatcgt ccgcataccc tgatcgacgc ttccggtctg gaagtcggtc
    24781 tgcctgaccg tcagatgggt aactccgaag taggccacgt taacctgggt gcaggccgca
    24841 tcgtgtatca ggacctgact cgtctggacg ttgaaatcaa agatcgcact ttctttgcta
    24901 atccggtgtt gactggtgcg gtagataaag cgaaaaacgc aggcaaagca gtacacatta
    24961 tgggtctgct ctccgcaggt ggtgtacaca gccacgaaga tcacatcatg gcgatggtag
    25021 aactggcagc tgaacgcggc gcagaaaaaa tctacctaca cgcattcctt gacggtcgcg
    25081 acactccgcc gcgcagtgct gaatcctcgc tgaaaaaatt cgaagagaaa tttgccgcgc
    25141 tgggtaaagg ccgcgtagcg tccatcattg gtcgttacta cgcgatggac cgcgataacc
    25201 gttgggatcg cgttgaaaaa gcttatgacc tgctgactct ggcgcaaggt gagttccagg
    25261 ccgataccgc cgttgccggt ttgcaggctg cttatgctcg cgacgaaaac gacgagttcg
    25321 tgaaagcgac cgttattcgt gcagaaggtc agccagatgc ggcaatggaa gacggcgatg
    25381 cgctgatttt catgaacttc cgtgctgacc gcgcacgtga aatcactcgt gcattcgtaa
    25441 atgccgattt tgacggcttc gcgcgtaaga aagtggttaa cgtcgatttc gtgatgctga
    25501 ccgaatacgc tgccgacatc aaaaccgcag tggcttaccc acccgcttcc ctggttaaca
    25561 ccttcggcga gtggatggcg aaaaacgaca aaactcagct gcgtatttcc gaaaccgaaa
    25621 aatatgccca cgttactttc ttcttcaacg gtggcgtaga agagtcgttc aaaggcgaag
    25681 atcgcattct gatcaactca ccgaaagtgg ctacctacga tctgcaaccg gaaatgagct
    25741 ccgcagagct gaccgaaaaa ctggttgcgg caatcaagag cggcaaatac gacaccatta
    25801 tctgtaacta tccgaacggc gacatggtag gtcacaccgg ggtgatggaa gcggcggtta
    25861 aagcggttga agcgctggat cactgcgtgg aagaagtcgc gaaagcggtt gagtccgtgg
    25921 gtggacaact gctgatcacc gctgaccacg gtaacgctga acagatgcgc gatccggcaa
    25981 cgggtcaggc acacacagca cacaccaacc tgccagttcc gctgatttac gttggtgata
    26041 agaacgtgaa agcggttgaa ggcggcaaac tttctgacat cgcgccgacc atgttgtcgc
    26101 tgatgggtat ggaaatcccg caagagatga ctggtaagcc gctgttcatc gtggaataat
    26161 ccctccccat gaggggaaag gcgattaata ccatgacacg ggccgtgaaa ccgcgcaggt
    26221 ttgcaatcag gcccatcatc tacgccagcg ttctgagcgc tggcgtattg ttgtgcgcct
    26281 tttccgccca cgcggatgag cgtgaccaac tcaaatccat tcaggctgac atcgccgcga
    26341 aagagcgcgc ggtacgccaa aagcaacaac aacgcgcaag cctgctcgca caattgaaaa
    26401 agcaggaaga agcgatctct gaagccaccc gtaaactgcg cgaaacgcaa aacacgctta
    26461 atcaactgaa taaacagatt gatgagatga acgcgtcgat tgccaaactg gagcagcaaa
    26521 aagccgccca ggagcgcagc ctcgccgcac aactggatgc cgcgtttcgt cagggtgaac
    26581 ataccggtat tcagctgatt ctcagcggtg aagaaagcca gcgtgggcag cggttacagg
    26641 cttatttcgg ctatctcaac caggcgcgac aagaaaccat tgctcagttg aaacaaacgc
    26701 gtgaagaagt cgctatgcag cgtgccgaac tggaagagaa acaaagcgag caacaaacgc
    26761 ttttatatga gcagcgcgcc caacaggcga agctgaccca ggcgttgagc gagcgtaaaa
    26821 agacgctggc agggctggag tcttccatcc agcaaggtca gcaacagttg agcgagctgc
    26881 gcgccaacga atcccgcctg cgtaacagca ttgcccgtgc ggaagccgcg gcgaaagcgc
    26941 gtgctgaacg tgaagcgcgc gaagcccagg cggttcgcga ccgccagaaa gaagcgacgc
    27001 gcaaaggcac cacctacaag ccgaccgaaa gcgaaaaatc gctgatgtcc cgtaccggtg
    27061 gtctgggcgc accgcgcggt caggcattct ggccggttcg cgggccaacg ctgcatcgct
    27121 atggcgagca gctacagggt gaattacgct ggaaagggat ggtgattggt gcctctgaag
    27181 gtactgaagt taaagcgatt gccgacggcc gggtgattct ggctgactgg ctgcaaggct
    27241 acggtctggt ggtggtggtt gagcatggta aaggcgacat gagtctttac ggctataatc
    27301 agagcgcact ggtgagcgtt ggttcgcagg ttcgcgcggg ccagccaatt gcactggtgg
    27361 gcagcagtgg cggtcagggt cggccttcac tctatttcga aattcgccgc cagggtcagg
    27421 cggtcaatcc acagccgtgg ttgggaagat aagttttgtt tccatttcgt cgtaacgttc
    27481 ttgcatttgc cgctctgttg gcgctctcct cccccgtaat tgctggcaaa cttgccatcg
    27541 tcattgatga ttttgggtat cgcccgcaca acgaaaacca ggtgctggcg atgccttccg
    27601 ctatctccgt cgctgtatta cccgattcac cgcacgccag agagatggcg accaaagcac
    27661 ataacagcgg tcacgaagtg ttgattcatc taccgatggc accactgagt aaacagccgc
    27721 tggagaaaaa tacgctacgc ccggagatga gcagcgacga aattgagcgc attattcgta
    27781 gtgcggtcaa taacgtgccc tatgccgtgg ggatcaacaa ccacatgggc agcaagatga
    27841 cctctaacct gtttggtatg cagaaagtga tgcaggcgct ggagcgttac aatctttact
    27901 ttctcgacag cgtaaccatc ggtaataccc aggcgatgcg cgccgcgcaa ggcactggcg
    27961 tgaaggtgat caaacggaag gtgttcctcg acgattcgca gaacgaagcg gacatccgtg
    28021 tgcaattcaa tcgcgcaatt gacctagcgc gtcgcaacgg ttcaaccatt gccattgggc
    28081 atcctcaccc ttcaacggta cgcgtgctac aacagctggt ttataacctg ccgccagaca
    28141 ttacgctggt gaaagccagc agcttgctga atgaaccgca ggttgatacg tctacaccgc
    28201 cgaaaaacac tgtgcccgac acaccgcgta atccgttccg tggcgtgaag ctgtgcaaac
    28261 cgaagaagcc gctggaacct gtttatgcta atcgcttctt tgaagtatta agcgaaagca
    28321 tcagccagag cacgctgatc gtttacttcc agcatcagtg gcaaggctgg ggcaaacagc
    28381 ccgaagcggc aaaacttaac gctagcgcaa attaaaggcg ctacgcgtaa tgcgatggtg
    28441 cgaccgcgtt ttgtcgcgcc attgccataa gcggaaagac cacagtaacg cctggtaacc
    28501 caccttcacg ctgcggacat tggtaatcag gcgcttatac ataccggaag taaatatctc
    28561 ggcaatcatc cgttggcggg taagaatatc cggctctttg cgcaccgcat ggcaaacacg
    28621 caatgcttcg taagttattt gctgatgaaa ttccggataa atcgtaattt tgtcagcata
    28681 atttcgattt aatttctcca gcaggcgggt aatcttaata tagtgacgtt gataattaag
    28741 gtttttattc ccttgtctat gcaa
//
LOCUS       DAHHTG010000056        28356 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.056, whole genome shotgun sequence.
ACCESSION   DAHHTG010000056 DAHHTG010000000
VERSION     DAHHTG010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28356)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28356)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28356
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.056"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            complement(<1..299)
                     /gene="tuf"
                     /locus_tag="NBI74_002354"
     CDS             complement(<1..299)
                     /gene="tuf"
                     /locus_tag="NBI74_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HCD1932678.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVEYDTPTRHYAHVDCPGHADYVKNMITGAAQM"
     gene            complement(370..2484)
                     /gene="fusA"
                     /locus_tag="NBI74_002355"
     CDS             complement(370..2484)
                     /gene="fusA"
                     /locus_tag="NBI74_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020455498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HCD1932679.1"
                     /translation="MARTTPIARYRNIGISAHIDAGKTTTTERILFYTGVNHKIGEVH
                     DGAATMDWMEQEQERGITITSAATTAFWSGMAKQYEPHRINIIDTPGHVDFTIEVERS
                     MRVLDGAVMVYCAVGGVQPQSETVWRQANKYKVPRIAFVNKMDRMGANFLKVVNQIKT
                     RLGANPVPLQLAIGAEEHFTGVVDLVKMKAINWNDADQGVTFEYEDIPADMVELANEW
                     HQNLIESAAEASEELMEKYLGGEELTEAEIKGALRQRVLNNEIILVTCGSAFKNKGVQ
                     AMLDAVIDYLPSPVDVPAINGILDDGKDTPAERHASDDEPFSALAFKIATDPFVGNLT
                     FFRVYSGVVNSGDTVLNSVKAARERFGRIVQMHANKREEIKEVRAGDIAAAIGLKDVT
                     TGDTLCDPDAPIILERMEFPEPVISIAVEPKTKADQEKMGLALGRLAKEDPSFRVWTD
                     EESNQTIIAGMGELHLDIIVDRMKREFNVEANVGKPQVAYRETIRQKVTDVEGKHAKQ
                     SGGRGQYGHVVIDMYPLEPGSNPKGYEFINDIKGGVIPGEYIPAVDKGIQEQLKAGPL
                     AGYPVVDMGIRLHFGSYHDVDSSELAFKLAASIAFKEGFKKAKPVLLEPIMKVEVETP
                     EENTGDVIGDLSRRRGMLKGQESEVTGVKIHAEVPLSEMFGYATQLRSLTKGRASYTM
                     EFLKYDEAPSNVAQAVIEARGK"
     gene            complement(2581..3051)
                     /gene="rpsG"
                     /locus_tag="NBI74_002356"
     CDS             complement(2581..3051)
                     /gene="rpsG"
                     /locus_tag="NBI74_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015873274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HCD1932680.1"
                     /translation="MPRRRVIGQRKILPDPKFGSELLAKFVNILMVDGKKSTAESIVY
                     SALETLAQRSGKSELEAFEVALENVRPTVEVKSRRVGGSTYQVPVEVRPVRRNALAMR
                     WIVEAARKRGDKSMALRLANELSDAAENKGTAVKKREDVHRMAEANKAFAHYRW"
     gene            complement(3148..3522)
                     /gene="rpsL"
                     /locus_tag="NBI74_002357"
     CDS             complement(3148..3522)
                     /gene="rpsL"
                     /locus_tag="NBI74_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005306278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HCD1932681.1"
                     /translation="MATVNQLVRKPRARKVAKSNVPALEACPQKRGVCTRVYTTTPKK
                     PNSALRKVCRVRLTNGFEVTSYIGGEGHNLQEHSVILIRGGRVKDLPGVRYHTVRGAL
                     DCSGVKDRKQARSKYGVKRPKA"
     gene            complement(3648..3935)
                     /gene="tusB"
                     /locus_tag="NBI74_002358"
     CDS             complement(3648..3935)
                     /gene="tusB"
                     /locus_tag="NBI74_002358"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusB"
                     /protein_id="HCD1932682.1"
                     /translation="MLHTLHRSPWLTDFAALLRLLSEGDELLLLQDGVTAAVDGNRYL
                     ESLRNAPIKVYALNEDLIARGLTGRISNDIIPIDYTDFVRLTVKHSSQMAW"
     gene            complement(3942..4301)
                     /gene="tusC"
                     /locus_tag="NBI74_002359"
     CDS             complement(3942..4301)
                     /gene="tusC"
                     /locus_tag="NBI74_002359"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusC"
                     /protein_id="HCD1932683.1"
                     /translation="MKRIAFVFSTAPHGTTAGREGLDALLATSALTDDLAVFFIADGV
                     FQLLPGQKPDAVLARDYIATFKLLGLYDIEQCWICAASLRERGLDPQTPFVVEATPLE
                     ADALRRELANYDVILRF"
     gene            complement(4301..4687)
                     /gene="tusD"
                     /locus_tag="NBI74_002360"
     CDS             complement(4301..4687)
                     /gene="tusD"
                     /locus_tag="NBI74_002360"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusD"
                     /protein_id="HCD1932684.1"
                     /translation="MRFAIVVTGPAYGTQQASSAFQFAQALIAEGHELSSVFFYREGV
                     YNANQLTSPASDEFDLVRGWQQLNAQHGVALNICVAAALRRGVVDETEAGRLGLASSN
                     LQPGFTLSGLGALAEASLTCDRVVQF"
     gene            complement(4687..5409)
                     /locus_tag="NBI74_002361"
     CDS             complement(4687..5409)
                     /locus_tag="NBI74_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCD1932685.1"
                     /translation="MSRSLLTNETSELDLLDQRPFDQTDFDILKSYEAVVDGLAMLIG
                     SHCEIVLHSLQDLKCSAIRIANGEHTGRKIGSPITDLALRMLHDMTGADSSVSKCYFT
                     RAKSGVLMKSLTIAIRNREQRVIGLLCINMNLDVPFSQIMSTFVPPETPDVGSSVNFA
                     SSVEDLVTQTLEFTIEEVNADRNVSNNAKNRQIVLNLYEKGIFDIKDAINQVADRLNI
                     SKHTVYLYIRQFKSGDFQGQDK"
     gene            complement(5576..6388)
                     /gene="fkpA"
                     /locus_tag="NBI74_002362"
     CDS             complement(5576..6388)
                     /gene="fkpA"
                     /locus_tag="NBI74_002362"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HCD1932686.1"
                     /translation="MKSLFKVTLLATTMAVALHAPITFAAEAAKPATTADSKAAFKND
                     DQKSAYALGASLGRYMENSLKEQEKLGIKLDKDQLIAGVQDAFADKSKLSDQEIEQTL
                     QAFEARVKSSAQAKMEKDAADNEAKGKEYREKFAKEKGVKTSSTGLVYQVVEAGKGEA
                     PKDSDTVVVNYKGTLIDGKEFDNSYTRGEPLSFRLDGVIPGWTEGLKNIKKGGKIKLV
                     IPPELAYGKAGVPGIPPNSTLVFDVELLDVKPAPKADAKPEADAKAADSAKK"
     gene            6609..6827
                     /locus_tag="NBI74_002363"
     CDS             6609..6827
                     /locus_tag="NBI74_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein SlyX"
                     /protein_id="HCD1932687.1"
                     /translation="MQDLSLEARLAELESRLAFQEITIEELNVTVTAHEMEMAKLRDH
                     LRLLTEKLKASQPSNIASQAEETPPPHY"
     gene            complement(6876..7466)
                     /gene="slyD"
                     /locus_tag="NBI74_002364"
     CDS             complement(6876..7466)
                     /gene="slyD"
                     /locus_tag="NBI74_002364"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HCD1932688.1"
                     /translation="MKVAKDLVVSLAYQVRTEDGVLVDESPVSAPLDYLHGHGSLISG
                     LETALEGHEVGDKFDVAVGANDAYGQYDENLVQRVPKDVFMGVDELQVGMRFLAETDQ
                     GPVPVEITAVEDDHVVVDGNHMLAGQNLKFNVEVVAIREATEEELAHGHVHGAHDHHH
                     DHDHDGCCGGHGHDHGHEHGGEGCCGGKGNGGCGCH"
     gene            complement(7561..7761)
                     /locus_tag="NBI74_002365"
     CDS             complement(7561..7761)
                     /locus_tag="NBI74_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheV family putative metal-binding protein"
                     /protein_id="HCD1932689.1"
                     /translation="MAIRKRFIAGAKCPACQAQDSMAMWRENNIDIVECVKCGHQMRE
                     ADKEARDHVRKDEQVIGIFHPD"
     gene            complement(7771..9576)
                     /gene="kefB"
                     /locus_tag="NBI74_002366"
     CDS             complement(7771..9576)
                     /gene="kefB"
                     /locus_tag="NBI74_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefB"
                     /protein_id="HCD1932690.1"
                     /translation="MEGSDFLLAGVLFLFAAVAAVPLASRLGIGAVLGYLLAGIAIGP
                     WGLGFISDVDEILHFSELGVVFLMFIIGLELNPSKLWQLRRSIFGVGAAQVLLSAALL
                     AGLLMLTDFAWQAAVVGGIGLAMSSTAMALQLMREKGMNRSESGQLGFSVLLFQDLAV
                     IPALALVPLLAGSADEHFDWMKIGMKVLAFVGMLIGGRYLLRPVFRFIAASGVREVFT
                     AATLLLVLGSALFMDALGLSMALGTFIAGVLLAESEYRHELETAIDPFKGLLLGLFFI
                     SVGMSLNLGVLYTHLLWVVISVVVLVAVKILVLYLLARLYGVRSSERMQFAGVLSQGG
                     EFAFVLFSSASSQRLFQGDQMALLLVTVTLSMMTTPLLMKLVDKWLSRQFNGPEEEDE
                     KPWVNDDKPQVIVVGFGRFGQVIGRLLMANKMRITVLERDISAVNLMRKYGYKVYYGD
                     ATQVDLLRSAGAEAAESIVITCNEPEDTMKLVEICQQHFPHLHILARARGRVEAHELL
                     QAGVTQFSRETFSSALELGRKTLVTLGMHPHQAQRAQLHFRRLDMRMLRELIPMHADT
                     VQISRAREARRELEEIFQREMQQERRQLDGWDEFE"
     gene            complement(9576..10127)
                     /gene="kefG"
                     /locus_tag="NBI74_002367"
     CDS             complement(9576..10127)
                     /gene="kefG"
                     /locus_tag="NBI74_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312229.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     ancillary protein KefG"
                     /protein_id="HCD1932691.1"
                     /translation="MSQPAKVLLLYAHPESQDSVANRVLLKPATQLSNVTVHDLYAHY
                     PDFFIDIPREQALLREHEVIVFQHPLYTYSCPALLKEWLDRVLSRGFASGPGGNQLAG
                     KYWRSVITTGEPESAYRYDALNRYPMSDVLRPFELAAGMCRMHWLSPIIIYWARRQSA
                     QELASHARAYGDWLANPLSPGGR"
     gene            10258..12171
                     /locus_tag="NBI74_002368"
     CDS             10258..12171
                     /locus_tag="NBI74_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCD1932692.1"
                     /translation="MIVFSSLQIRRGVRVLLDNATATINPGQKVGLVGKNGCGKSTLL
                     ALLKNEISADGGSYTFPGSWQLAWVNQETPALPQAALEYVIDGDREYRQLEAQLHDAN
                     ERNDGQAIATIHGKLDAIDAWSIRSRAASLLHGLGFSNEQLERPVSDFSGGWRMRLNL
                     AQALICRSDLLLLDEPTNHLDLDAVIWLEKWLKSYQGTLILISHDRDFLDPIVDKIIH
                     IEQQSMFEYTGNYSSFEVQRATRLAQQQAMYESQQERVAHLQSYIDRFRAKATKAKQA
                     QSRIKMLERMELIAPAHVDNPFRFSFRAPESLPNPLLKMEKVSAGYGDRIILDSIKLN
                     LVPGSRIGLLGRNGAGKSTLIKLLAGELAPVSGEIGLAKGIKLGYFAQHQLEYLRADE
                     SPIQHLARLAPQELEQKLRDYLGGFGFQGDKVTEETRRFSGGEKARLVLALIVWQRPN
                     LLLLDEPTNHLDLDMRQALTEALIEFEGALVVVSHDRHLLRSTTDDLYLVHDRKVEPF
                     DGDLEDYQQWLSDVQKQENQADEAPKENANSAQARKDQKRREAELRAQTQPLRKEIAR
                     LEKEMEKLNAQLAQAEEKLGDSELYDQSRKAELTACLQQQASAKSGLEECEMAWLEAQ
                     EQLEQMLLEGQSN"
     gene            12171..13193
                     /locus_tag="NBI74_002369"
     CDS             12171..13193
                     /locus_tag="NBI74_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HCD1932693.1"
                     /translation="MAQITTTDANEFSSSAEFTPMRGFSNCHLQTMLPRLFRRKVKFT
                     PHWHRLELPDGDFVDLAWSEDPAQARHKPRLVVFHGLEGSLNSPYAHGLVEAAQKRGW
                     LGVVMHFRGCSGEPNRMHRIYHSGETEDASWFLRWLQREFGHAPTAAVGYSLGGNMLA
                     CLLAKEGNDLPIDAAVIVSAPFMLEACSYHMEKGFSRIYQRYLLNLLKANAARKLAAY
                     PGTLPINLAQLKSVRRIREFDDLITARIHGYADAIDYYRQCSAMPMLNRIAKPTLIIH
                     AKDDPFMDHQVFPKPESLPPQVEYQLTEHGGHVGFIGGTLLHPQMWLESRIPDWLTTY
                     LEAKSC"
     gene            13187..13405
                     /locus_tag="NBI74_002370"
     CDS             13187..13405
                     /locus_tag="NBI74_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheU family protein"
                     /protein_id="HCD1932694.1"
                     /translation="MLIPWQDLSPETLENLIESFVLREGTDYGEHERTLEQKVADVKR
                     QLQCGEAVLVWSELHETVNIMPRSQFRE"
     gene            13459..14328
                     /locus_tag="NBI74_002371"
     CDS             13459..14328
                     /locus_tag="NBI74_002371"
                     /EC_number="2.7.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribulokinase"
                     /protein_id="HCD1932695.1"
                     /translation="MSAKHPVIAVTGSSGAGTTTTSLAFRKIFAQLNLHAAEVEGDSF
                     HRYTRPEMDMAIRKARDAGRHISYFGPEANDFGLLEQTFIEYGQSGKGKSRKYLHTYD
                     EAVPWNQVPGTFTPWQSLPEPTDVLFYEGLHGGVVTPQHNVAQHVDLLVGVVPIVNLE
                     WIQKLIRDTSERGHSREAVMDSVVRSMEDYINYITPQFSRTHLNFQRVPTVDTSNPFA
                     AKGIPSLDESFVVIHFRNLEGIDFPWLLAMLQGSFISHINTLVVPGGKMGLAMELIML
                     PLVQRLMEGKKIE"
     gene            complement(14383..14787)
                     /locus_tag="NBI74_002372"
     CDS             complement(14383..14787)
                     /locus_tag="NBI74_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HCD1932696.1"
                     /translation="MQARVKWVEGLTFLGESASGHQILMDGNSGDKAPSPMEMVLMAA
                     GGCSAIDVVSILQKGRQDVVDCEVKLTSERREEAPRLFTHINLHFIVTGRDLKDAAVA
                     RAVDLSAEKYCSVALMLEKAVNITHSYEVVAA"
     gene            15089..15721
                     /gene="crp"
                     /locus_tag="NBI74_002373"
     CDS             15089..15721
                     /gene="crp"
                     /locus_tag="NBI74_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cAMP-activated global transcriptional regulator
                     CRP"
                     /protein_id="HCD1932697.1"
                     /translation="MVLGKPQTDPTLEWFLSHCHIHKYPSKSTLIHQGEKAETLYYIV
                     KGSVAVLIKDEEGKEMILSYLNQGDFIGELGLFEEGQERSAWVRAKTACEVAEISYKK
                     FRQLIQVNPDILMRLSAQMARRLQVTSEKVGNLAFLDVTGRIAQTLLNLAKQPDAMTH
                     PDGMQIKITRQEIGQIVGCSRETVGRILKMLEDQNLISAHGKTIVVYGTR"
     gene            15772..17862
                     /locus_tag="NBI74_002374"
     CDS             15772..17862
                     /locus_tag="NBI74_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312236.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HCD1932698.1"
                     /translation="MWRRLIYHPDINYALRQTLVLCLPVAVGLMLGELRFGLLFSLVP
                     ACCNIAGLDTPHKRFFKRLIIGASLFATCSLLTQVLLAKDVPLPFLLTGLTLVLGVTA
                     ELGPLHAKLLPASLLAAIFTLSLAGYMPVWEPLLIYALGTLWYGLFNWFWFWIWREQP
                     LRESLSLLYRELADYCEAKYSLLTQHTDPEKALPPLLVRQQKAVDLITQCYQQMHMLS
                     AQNNTDYKRMLRIFQEALDLQEHISVSLHQPEEVQKLVERSHAEEVIRWNAQTVAARL
                     RVLADDILYHRLPTRFTMEKQIGALEKIARQHPDNPVGQFCYWHFSRIARVLRTQKPL
                     YARDLLADKQRRMPLLPALKSYLSLKSPALRNAGRLSVMLSVASLMGTALHLPKSYWI
                     LMTVLLVTQNGYGATRLRIVNRSVGTVVGLIIAGVALHFKIPEGYTLTLMLITTLASY
                     LILRKNYGWATVGFTITAVYTLQLLWLNGEQYILPRLIDTIIGCLIAFGGTVWLWPQW
                     QSGLLRKNAHDALEAYQDAIRLILSEDPQPTPLAWQRMRVNQAHNTLYNSLNQAMQEP
                     AFNSHYLADMKLWVTHSQFIVEHINAMTTLAREHRALPPELAQEYLQSCEIAIQRCQQ
                     RLEYDEPGSSGDANIMDAPEMQPHEGAAGTLEQHLQRVIGHLNTMHTISSMAWRQRPH
                     HGIWLSRKLRDSKA"
     gene            complement(17929..19149)
                     /gene="argD/dapC"
                     /locus_tag="NBI74_002375"
     CDS             complement(17929..19149)
                     /gene="argD/dapC"
                     /locus_tag="NBI74_002375"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     acetylornithine/succinyldiaminopimelate transaminase"
                     /protein_id="HCD1932699.1"
                     /translation="MAIEQTAITRATFDEVILPIYAPAEFIPVKGQGSRIWDQQGKEY
                     VDFAGGIAVTALGHCHPALVNALKTQGETLWHISNVFTNEPALRLGRKLIEATFAERV
                     VFMNSGTEANETAFKLARHYACVRHSPFKTKIIAFHNAFHGRSLFTVSVGGQPKYSDG
                     FGPKPADIIHVPFNDLHAVKAVMDDHTCAVVVEPIQGEGGVTAATPEFLQGLRELCDQ
                     HQALLVFDEVQCGMGRTGDLFAYMHYGVTPDILTSAKALGGGFPISAMLTTAEIASAF
                     HPGSHGSTYGGNPLACAVAGAAFDIINTPEVLEGIQAKRQRFVDHLQKIDQQYDVFSD
                     IRGMGLLIGAELKPQYKGRARDFLYAGAEEGVMVLNAGPDVMRFAPSLVVEDADIDEG
                     MHRFAHAVAKVVGA"
     gene            complement(19235..19798)
                     /gene="pabA"
                     /locus_tag="NBI74_002376"
     CDS             complement(19235..19798)
                     /gene="pabA"
                     /locus_tag="NBI74_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 2"
                     /protein_id="HCD1932700.1"
                     /translation="MILLIDNYDSFTWNLYQYFCELGADVLVKRNDALTLADIDALKP
                     QKIVISPGPCTPDEAGISLDVIRHYAGRLPILGVCLGHQAMAQAFGGKVVRAAKVMHG
                     KTSPITHNGVGVFKGLANPLTVTRYHSLVVEPDSLPECFEVTAWSETREIMGIRHRQW
                     DLEGVQFHPESILSEQGHQLLANFLHR"
     gene            complement(19830..20432)
                     /locus_tag="NBI74_002377"
     CDS             complement(19830..20432)
                     /locus_tag="NBI74_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative adenosine monophosphate-protein
                     transferase Fic"
                     /protein_id="HCD1932701.1"
                     /translation="MSDKFGEGRDPYLYPGLDIMRNKLNIRQQQRLEQAAYEMTALRA
                     ATIELGPLVRGLPHLRTIHRQLYQDIFDWAGQLREVDIYQGDTPFCHFAYLEKEGNAL
                     MQDLEEEKYLTSLEKDKFVERLAHYYCEINVLHPFRVGSGLAQRIFFEQLAIHAGYQL
                     SWQGIEKEAWNQANQNGAMGDLTALQMIFSKVVSEVGESE"
     gene            complement(20422..20589)
                     /locus_tag="NBI74_002378"
     CDS             complement(20422..20589)
                     /locus_tag="NBI74_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfG family protein"
                     /protein_id="HCD1932702.1"
                     /translation="MKKLTDKQKSRLWELQRNRNFQASRRLEGVEMPLVTLTAAEALA
                     RLEALRRHYER"
     gene            complement(20694..21266)
                     /gene="ppiA"
                     /locus_tag="NBI74_002379"
                     /gene_synonym="rotA"
     CDS             complement(20694..21266)
                     /gene="ppiA"
                     /locus_tag="NBI74_002379"
                     /gene_synonym="rotA"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase A"
                     /protein_id="HCD1932703.1"
                     /translation="MFKSTLAAMAAVFALSALSPAAMAAKGDPHVLLTTSAGNIELEL
                     DKQKAPVSVQNFVDYVNSGFYNNTTFHRVIPGFMIQGGGFTEQMQQKKPNPPIKNEAD
                     NGLRNTRGTIAMARTADKDSATSQFFINVADNAFLDHGQRDFGYAVFGKVVKGMDVAD
                     KISQVPTHDVGPYQNVPSKPVVILSAKVLP"
     gene            21537..22718
                     /gene="tsgA"
                     /locus_tag="NBI74_002380"
     CDS             21537..22718
                     /gene="tsgA"
                     /locus_tag="NBI74_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter TsgA"
                     /protein_id="HCD1932704.1"
                     /translation="MTNSNRIKLTWISFLSYALTGALVIVTGMVMGNIADYFNLPVSS
                     MSNTFTFLNAGILISIFLNAWLMEIVPLKTQLRFGFLLMVLAVAGLMFSHSLALFSAA
                     MFILGVVSGITMSIGTFLVTQMYEGRQRGSRLLFTDSFFSMAGMIFPMIAAFLLARSI
                     EWYWVYACIGLVYVAIFILTFGCEFPALGKHAPKTDAPVEKEKWGIGVLFLSVAALCY
                     ILGQLGFISWVPEYAKGLGMSLNDAGTLVSNFWMSYMVGMWAFSFILRFFDLQRILTV
                     LAGLAAILMYVFNTGTPAHMAWSILALGFFSSAIYTTIITLGSQQTKVPSPKLVNFVL
                     TCGTIGTMLTFVVTGPIVEHSGPQAALLTANGLYAVVFVMCFLLGFVSRHRQHNTLTS
                     H"
     gene            22980..25523
                     /gene="nirB"
                     /locus_tag="NBI74_002381"
     CDS             22980..25523
                     /gene="nirB"
                     /locus_tag="NBI74_002381"
                     /EC_number="1.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-nitrite reductase large subunit"
                     /protein_id="HCD1932705.1"
                     /translation="MSKVRLAIIGNGMVGHRFIEDLLDKSDAANFDITVFCEEPRIAY
                     DRVHLSSYFSHHTAEELSLVREGFYEKHGIKVLVGERAITINRQEKVIHSSAGRTVFY
                     DKLIMATGSYPWIPPIKGSDTQDCFVYRTIEDLNAIESCARRSKRGAVVGGGLLGLEA
                     AGALKNLGIETHVIEFAPMLMAEQLDQMGGEQLRRKIESMGVRVHTSKNTLEIVQEGV
                     EARKTMRFADGSELEVDFIVFSTGIRPRDKLATQCGLDVAPRGGIVINDSCQTSDPDI
                     YAIGECASWNNRVFGLVAPGYKMAQVAVDHILGSENAFEGADLSAKLKLLGVDVGGIG
                     DAHGRTPGARSYVYLDESKEIYKRLIVSEDNKTLLGAVLVGDTSDYGNLLQLVLNAIE
                     LPENPDSLILPAHSGSGKPSIGVDKLPDSAQICSCFDVTKGDLIAAINKGCHTVAALK
                     AETKAGTGCGGCIPLVTQVLNAELAKQGIEVNNNLCEHFAYSRQELFHLIRVEGIKTF
                     EELLAKHGKGYGCEVCKPTVGSLLASCWNEYILKPEHTPLQDSNDNFLANIQKDGTYS
                     VIPRSPGGEITPEGLMAVGRIAREFNLYTKITGSQRLAMFGAQKDDLPEIWRQLIEAG
                     FETGHAYAKALRMAKTCVGSTWCRYGVGDSVGLGVELENRYKGIRTPHKMKFGVSGCT
                     RECSEAQGKDVGIIATEKGWNLYVCGNGGMKPRHADLLAADIDRETLIKYLDRFMMFY
                     IRTADKLTRTAPWLENLEGGIDYLKAVIIDDKLGLNAHLEEEMARLREAVVCEWTETV
                     NTPSAQTRFKHFINSDKRDPNVQMVPEREQHRPATPYERIPVTLVEDNA"
     gene            25520..25846
                     /gene="nirD"
                     /locus_tag="NBI74_002382"
     CDS             25520..25846
                     /gene="nirD"
                     /locus_tag="NBI74_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase small subunit NirD"
                     /protein_id="HCD1932706.1"
                     /translation="MSQWKDICKIDDILPETGVCALLGDEQVAIFRPYHSDQVFAISN
                     IDPFFESSVLSRGLIAEHQGELWVASPLKKQRFRLSDGLCMEDEQFSVKHYDARVKDG
                     VVQLRG"
     gene            25973..26779
                     /gene="nirC"
                     /locus_tag="NBI74_002383"
     CDS             25973..26779
                     /gene="nirC"
                     /locus_tag="NBI74_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite transporter NirC"
                     /protein_id="HCD1932707.1"
                     /translation="MFTDTITKCAANAARIARLSANNPLGFWVSSAMAGAYVGLGIIL
                     IFTLGNLLDPSVRPLVMGATFGIALTLVIIAGSELFTGHTMFLTFGVKAGTISHGQMW
                     AILPQTWLGNLVGSVFVAMLYSWGGGSLLPVDTSIVHSVALAKTTAPAMVLFFKGALC
                     NWLVCLAIWMALRTEGAAKFIAIWWCLLAFIASGYEHSIANMTLFALSWFGNHSEAYT
                     LAGIGHNLLWVTLGNTLSGAVFMGLGYWYATPKANRPVADKFNQTETAAG"
     gene            26798..28171
                     /gene="cobA"
                     /locus_tag="NBI74_002384"
     CDS             26798..28171
                     /gene="cobA"
                     /locus_tag="NBI74_002384"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HCD1932708.1"
                     /translation="MDHLPIFCQLRDRDCLIVGGGDVAERKARLLLDAGARLTVNALA
                     FIPQFTAWADAGMLTLVEGPFDESLLDTCWLAIAATDDDALNQRVSEAAEARRIFCNV
                     VDAPKAASFIMPSIIDRSPLMVAVSSGGTSPVLARLLREKLESLLPLHLGQVAKYAGQ
                     LRGRVKQQFATMGERRRFWEKLFVNDRLAQSLANNDQKAITETTEQLINEPLDHRGEV
                     VLVGAGPGDAGLLTLKGLQQIQQADVVVYDRLVSDDIMNLVRRDADRVFVGKRAGYHC
                     VPQEEINQILLREAQKGKRVVRLKGGDPFIFGRGGEELETLCNAGIPFSVVPGITAAS
                     GCSAYSGIPLTHRDYAQSVRLITGHLKTGGELDWENLAAEKQTLVFYMGLNQAATIQQ
                     KLIEYGMPGEMPVAIVENGTAVTQRVIDGTLTQLGELAQQMNSPSLIIIGRVVGLRDK
                     LNWFSNH"
BASE COUNT         6861 a         7584 c         7157 g         6754 t
ORIGIN      
        1 tccatctgag cagcaccggt gatcatgttt ttaacatagt cggcgtgccc cgggcagtct
       61 acgtgtgcgt agtgacgggt cggggtgtcg tattcaacgt gagaagtgtt gatggtgata
      121 ccacgagcct tttcttccgg cgcgttatcg atctggtcga atgcacgagc agcaccgccg
      181 taggttttag ccagtacggt ggtgattgca gcggtcagag tagttttacc gtggtcaacg
      241 tggccgatag taccaacgtt aacgtgcggt tttgtacgtt caaatttttc tttagacacg
      301 gctatattcc ttactatagt gctctcccct tcaggagaga gcacgggact ttggtattaa
      361 cccttaggct tatttaccac gggcttcaat tacggcctga gcaacgttac tcggcgcttc
      421 atcatacttc aggaattcca tagtgtatga tgcgcgacct ttggtcagag aacgcagctg
      481 agttgcatat ccgaacattt cagacagcgg tacttcagcg tggatcttaa cgccagtaac
      541 ttcagattcc tgacctttga gcataccacg acgacggctc aagtcaccga taacgtcacc
      601 ggtgttctct tcaggagttt ctacttcaac cttcatgatc ggctcaagca gaactggttt
      661 cgctttctta aagccttctt taaaggcgat agaagcagcc agtttaaacg ccagttcaga
      721 ggagtcaacg tcatggtaag aaccgaagtg cagacgaata cccatgtcta ctaccgggta
      781 gcctgccagc ggacctgctt tcagctgttc ctggatacct ttatcaacgg ccgggatgta
      841 ttcgccaggg attacaccac ctttaatgtc gttgatgaac tcgtagcctt tcgggtttga
      901 acccggctcc agcgggtaca tgtcgataac aacatgacca tactgaccac gaccaccaga
      961 ctgtttcgcg tgtttacctt caacatcggt aactttctgg cggatagttt cacggtaagc
     1021 aacctgcggt ttacctacgt tcgcttcaac gttgaattca cgcttcatac ggtcaacgat
     1081 gatgtcgagg tgcagttcgc ccatacccgc gatgatggtc tggttagatt cttcgtcagt
     1141 ccatacacgg aaagacgggt cttctttagc cagacggccc agagccagac ccattttttc
     1201 ctggtcagct ttggttttcg gttcaactgc gatggagatt accggctcag ggaattccat
     1261 acgttccaga atgatcggcg catccgggtc acacagggtg tcaccagtgg ttacgtcttt
     1321 cagaccgatt gcagctgcga tgtcgcccgc gcgaacttct ttgatctctt cacgtttgtt
     1381 agcgtgcatc tgaacgatac gaccgaaacg ctcacgtgca gctttcacgg agttcagtac
     1441 ggtatcacca gagttaacca caccggagta cacacggaag aaggtcaggt taccaacaaa
     1501 cgggtcggta gcgattttga acgccagtgc agagaacggc tcgtcatcac ttgcgtgacg
     1561 ctcagccgga gtgtctttac cgtcgtccag gataccgttg atcgcaggta cgtcaaccgg
     1621 ggatggcagg taatcaatta ccgcatccag catcgcctga acacctttgt tcttgaacgc
     1681 agaaccacag gttaccagga tgatttcgtt gttcagaacg cgctgacgca gagcaccttt
     1741 gatttctgct tcagtcagtt cttcaccacc cagatatttt tccatcagct cttcagaagc
     1801 ttcagctgcg gattcgatca ggttctggtg ccattcgtta gccagttcaa ccatgtctgc
     1861 cgggatatct tcgtattcga aggttacgcc ctggtcagcg tcgttccagt tgatagcttt
     1921 cattttcacc aggtcaacaa caccggtgaa atgttcttca gcaccaatcg ccagctgcag
     1981 cggaaccggg ttcgcgccca gacgggtttt gatctggtta acaactttca ggaagttcgc
     2041 acccatgcgg tccattttgt taacgaacgc aatacgcgga actttatatt tgtttgcctg
     2101 acgccatacg gtttcagact gcggctgaac accaccaact gcgcagtaaa ccattaccgc
     2161 accatcgaga acacgcatgg aacgttctac ttcgattgtg aagtcaacgt gccccggggt
     2221 gtcgatgatg ttgatgcgat gcggctcata ctgcttagcc ataccagacc agaatgcagt
     2281 agtcgcagcg gaagtgatgg taataccacg ttcctgctcc tgctccatcc agtccatggt
     2341 tgcagcgccg tcatgaactt caccgatttt atggtttaca ccggtgtaga acagaatacg
     2401 ttcggtagta gtggttttac cggcgtcgat gtgcgcactg ataccgatgt tacggtagcg
     2461 tgcgatgggt gttgtacgag ccatttgttt cctcgtttat cttttaggcg ttcaatttaa
     2521 gtagcccaaa gcgggctgct tactggaagc gcccgcctgg tgactaaaac tccgaaggga
     2581 ttaccaacgg tagtgtgcga acgccttgtt ggcttcggcc atacggtgaa cgtcttcacg
     2641 tttcttaact gcagtacctt tgttttctgc agcatcagaa agttcgttcg ccaggcgcag
     2701 agccatggat ttatcaccgc gtttacgagc agcttcaacg atccaacgca ttgccagagc
     2761 attacgacga accggacgga cttcaactgg tacctgataa gtagaaccac caacgcggcg
     2821 agacttaact tctacagtcg ggcgcacgtt ttcgagagct acttcgaatg cttccagttc
     2881 agatttacca gagcgctgag ccagggtctc cagcgcgctg tatacgatag attcagcagt
     2941 agatttttta ccatctacca tcaggatatt tacaaattta gccagcagtt ctgatccgaa
     3001 cttcggatcc ggcagaattt tacgctgacc aatgacgcga cgacgtggca tggaaatact
     3061 ccgttgttaa ttcaggattg tccaaaactc tacgagttta gtttgacatt taagttaaaa
     3121 cgtttggcct tacttaacgg agaaccatta agccttagga cgcttcacgc catacttgga
     3181 acgagcctgc ttacggtctt taacaccgga gcagtcaagc gcaccacgta cggtgtggta
     3241 acgaacaccc gggaggtctt taacacgacc gccacggatc aggatcacgg agtgctcctg
     3301 caagttgtga ccttcaccac cgatgtagga agtcacttcg aaaccgttag tcagacgaac
     3361 acggcatact ttacgcagcg cggagttcgg ttttttagga gtggtagtat atacacgagt
     3421 acatacgcca cgtttttgcg ggcatgcttc cagcgcaggc acgttgcttt tcgcaacttt
     3481 gcgagcacgt ggtttgcgta ccagctggtt aactgttgcc attaaatagc tcctggtttt
     3541 agcttttgct tcgtaaacac gtaataaaac gtcctcacac aatatgagga cgccgaattt
     3601 tagggcgatg ccgaaaaggt gtcaagaaat atacaacgat cccgccatca ccaggccatc
     3661 tggctggagt gcttaaccgt aagtctgacg aaatcagtat agtcaatggg aatgatgtcg
     3721 ttcgaaattc gaccagtcaa accgcgggca ataaggtctt cgttcagagc atagacctta
     3781 atgggggcat tacgcagact ttcaaggtag cggttgccgt caactgcggc agttacgcca
     3841 tcttgcaata atagcagttc gtcaccttca ctgagcagac gcagcagcgc agcaaagtcc
     3901 gtcagccagg gtgaacgatg taatgtgtgc agcataacgc ctcaaaacct caaaataaca
     3961 tcgtagttgg cgagttcgcg gcgtaaggca tctgcttcga gcggcgtggc ttcgaccaca
     4021 aagggtgtct gcggatctaa cccacgttcg cgcagtgaag ccgcgcaaat ccagcactgt
     4081 tcaatatcgt acagacccaa caatttaaaa gtggcaatgt aatcacgcgc cagcactgca
     4141 tcgggctttt gtcctggcag cagctgaaaa acgccatcag caataaagaa gacggccaga
     4201 tcgtcagtta atgcggaagt tgccagtaaa gcatctaaac cttcccggcc tgcagttgta
     4261 ccatgaggcg cagtagaaaa aacaaacgca attcgtttca tcagaactgt accaccctgt
     4321 cacaggtcag cgaggcttcc gccagcgcgc caagtccgct taaggtaaat cccggctgaa
     4381 ggtttgacga agcaagcccc agtcttccgg cctccgtttc atcaacaacg ccacggcgta
     4441 atgctgccgc tacgcagata ttcagcgcca caccatgttg cgcattcaac tgttgccagc
     4501 cgcgcacaag gtcaaattcg tcgcttgccg gtgaggtcaa ttggttagcg ttatagaccc
     4561 cttcccgata gaaaaaaacg ctgcttaact catggccttc tgctatcagc gcctgcgcaa
     4621 actgaaaagc actactcgcc tgttgcgtac cgtatgctgg cccggtcacc acgatggcaa
     4681 aacgcattac ttatcttgcc cctggaaatc accgctcttg aactggcgga tgtagagata
     4741 gacagtgtgt ttggagatgt tcaggcggtc agcaacctgg ttgatcgcgt ctttaatatc
     4801 gaagatccct ttctcgtaga gatttagcac gatctgacga tttttggcgt tattagaaac
     4861 attgcgatcg gcattcactt cttcgatggt gaactccagc gtttgggtaa ccagatcttc
     4921 aacagaagag gcaaagttga cgcttgaacc gacatccgga gtttccggcg gcacaaaagt
     4981 gctcataatc tgcgagaagg gaacatcaag attcatattg atgcacagca gaccaattac
     5041 acgctgttcg cggttacgaa tcgcgatagt cagggacttc attaatacgc cgcttttggc
     5101 gcgagtaaag tagcatttag aaacgctgct atccgctccc gtcatatcgt gcagcatacg
     5161 tagcgccagg tcagtaattg gcgaaccaat cttccggcct gtatgttcac cgttagcaat
     5221 gcgaatggct gaacatttta gatcctgcaa agagtgcaaa acgatttcac agtgggagcc
     5281 aataagcatc gctaacccgt ccaccaccgc ttcgtaggat ttcagaatat caaaatcggt
     5341 ctggtcaaaa ggacgttgat ccagtaaatc caactcactg gtttcgttgg ttaaaagcga
     5401 cctggacatg aaaaaaaaca ctccttttca ggagcctgtc gttaactttt cagggcaggc
     5461 tcattaatga tgcgggtaac taaattaata cagcggaggt tccgctttcc agcactaatt
     5521 atatccggcc tgtaataaaa aaaccgccgc ctggtcaggc ggcggttctt aatgcttatt
     5581 ttttagcaga gtctgcggct ttcgcatcag cttccggctt tgcatcagcc ttcggcgctg
     5641 gtttcacatc cagcagctct acgtcaaaca ccagggtaga attcggtggg atccccggaa
     5701 cacccgcttt gccgtaagcc agttctggtg gaataaccag tttgatctta ccgcctttct
     5761 tgatgttctt cagaccttct gtccaacccg gaataacacc gtccagacgg aaagagagcg
     5821 gttcaccacg ggtgtaagag ttgtcgaact ctttaccgtc gatcagcgta cctttgtagt
     5881 tcactacaac agtatcgctg tctttcggtg cttcgccttt accggcttct actacctgat
     5941 aaaccagacc agtggaagag gttttcacac ctttctcttt ggcaaatttc tcgcggtact
     6001 ctttaccttt tgcttcgtta tcagccgcgt ctttttccat cttcgcctga gcagaagact
     6061 tcacgcgagc ttcgaatgct tgcagagtct gttcgatctc ttggtcggaa agtttgctct
     6121 tatcagcaaa tgcatcctga acaccagcga tcagctgatc tttatccagt ttgatgccca
     6181 gtttttcttg ttctttaaga gagttttcca tgtaacgacc cagcgaagca cccagtgcat
     6241 aagctgattt ctgatcgtca tttttgaacg ctgctttgct gtcagcagtt gtagcaggtt
     6301 ttgcagcttc agcagcaaaa gtgattggtg catgcagggc aacggccatt gtggtcgcca
     6361 gcagcgttac tttaaacagt gatttcatcc atatctccag gatcggggca tctcacccca
     6421 gggttaacta ttatcagaag ggtactataa agcgttgtcg aacaaatcta catacagaca
     6481 cgccctatta tcatctattt tcagactctt tttgtttaaa ttagtttcga tgaccgcgaa
     6541 atgagtgctg tctcgggcag caaagttaag tagaatccgc ggcgaccatt cgacaaaaga
     6601 ggtgaatcat gcaggattta tcattggaag cacgcctggc agagctggag agccgactgg
     6661 cttttcagga aatcaccatt gaagaactga acgtcacggt gaccgctcat gaaatggaga
     6721 tggcgaaact gcgcgatcat ctgcgtctgc tgaccgagaa gttaaaagcc agccagccgt
     6781 cgaacatcgc gtcgcaggct gaagaaacgc ctccgccaca ttattgaggc gtaaaaaaag
     6841 cgggaattcc ccgctttttt gtcacctttt cggtattagt ggcaaccgca accgccgttg
     6901 cctttaccgc cacagcagcc ttcgccaccg tgttcatgac cgtgatcgtg gccatgacca
     6961 ccgcagcaac cgtcgtggtc gtgatcgtgg tggtgatcgt gcgcgccgtg aacgtgacca
     7021 tgagccagtt cttcttcggt cgcttcgcga atcgccacaa cttcaacgtt gaatttcagg
     7081 ttctgaccag ccagcatgtg gttaccatca accactacgt gatcgtcttc aaccgcagtg
     7141 atttcaaccg gtaccggacc ctggtcggtt tcagccagga aacgcatacc tacctgcagt
     7201 tcatcaacgc ccataaatac gtctttagga acgcgctgca ccaggttttc gtcgtactga
     7261 ccgtaagcgt cattcgcgcc aacagcgaca tcaaatttgt cgccaacttc atgaccttcc
     7321 agcgccgttt ccaggccaga gatcagggaa ccgtgaccat gcaggtagtc cagcggcgca
     7381 ctcaccggag actcatcaac caacacaccg tcttctgtac gtacctgata ggccaggctg
     7441 accaccaggt cttttgctac tttcatgata tctcctgagc atgggaagaa tagtggcgca
     7501 gattgtagcg gaattctgca gccgtgtact cactagctta aaaaaactcg gcgcatatcg
     7561 ctagtccgga tgaaaaatcc cgatcacttg ctcatctttg cgaacgtgat cgcgggcttc
     7621 tttgtctgct tctcgcatct gatgtccaca cttaacacat tcaacgatat caatattatt
     7681 ttcgcgccac atcgccattg aatcctgagc ctgacaggcc gggcattttg cgcccgcaat
     7741 aaaacgtttt cggattgcca tctttaccct ctactcaaat tcatcccagc cgtccagctg
     7801 gcgtcgttct tgttgcattt cacgctggaa aatctcttcc agttcgcgtc gggcttccct
     7861 ggcgcgagaa atttgtacgg tatcagcatg catcgggatt aactcccgca gcattcgcat
     7921 atccaggcgg cgaaaatgca gttgcgcgcg ctgcgcctga tgcggatgca tgccaagcgt
     7981 gaccagcgtc ttgcgcccca gctctaacgc actggagaat gtttcacggg aaaactgcgt
     8041 cacccctgcc tgtaataact catgcgcttc cacacgtccg cgcgctcgcg caagaatatg
     8101 caaatgcgga aagtgctgct ggcatatttc caccagcttc atagtgtctt ccggctcgtt
     8161 acaggtaatg acgatagact cagcggcctc tgcacccgca gaacgtaaaa gatctacctg
     8221 cgtggcgtcg ccgtaataaa ctttgtagcc atatttgcgc atcaggttaa cggcactgat
     8281 atcccgctcc agtacagtaa tgcgcatttt atttgccatc agcaaacgac caatcacctg
     8341 accaaaacgc ccaaagccca cgacaataac ctgcggttta tcatcgttga cccatggttt
     8401 ttcgtcttct tcttccggtc cgttaaactg gcgggatagc catttatcta ccagcttcat
     8461 cagcagcggc gtggtcatca tggaaagcgt caccgtcacc agcaacagcg ccatctggtc
     8521 gccctggaat aagcgttgtg aagaagcgga agaaaagagg acaaaggcaa actcaccgcc
     8581 ctgactcaac acgccagcaa actgcatccg ctctgagcta cgcacgccat acaatcgcgc
     8641 cagcagatac agcacgagaa ttttcaccgc caccagcaca accacactta tcactaccca
     8701 caacagatgg gtataaagca ccccgaggtt gagtgacatg ccgacagaga taaagaacaa
     8761 accgagcagc aagcctttga agggatcgat agccgtttcc agttcatggc gatattcact
     8821 ttccgccagt agcacacccg caataaacgt accgagcgcc atcgacaacc ccagcgcatc
     8881 cataaacaat gcggaaccca acaccagcag cagcgtcgcg gcggtgaaca cttcccgcac
     8941 accagaagct gcaataaaac ggaataccgg acgcagtaaa tagcgcccgc caatcagcat
     9001 gccgacaaac gccagcacct tcatgccgat cttcatccag tcgaaatgtt cgtctgccga
     9061 acccgccaac aacggcacta acgccagtgc cgggatcacc gccagatcct gaaacagcag
     9121 taccgagaaa cctaactggc cggattcgct gcgattcatc cctttttcac gcatcaactg
     9181 caatgccatt gcggttgaag acatcgcaag gccaatgcca ccgaccaccg ccgcctgcca
     9241 ggcgaaatcc gtcagcatca ataatcccgc cagcaacgcc gcgcttaaca gcacctgtgc
     9301 cgcgcctacg ccaaaaatcg aacgccgcag ttgccaaagc ttggagggat tcaactcaag
     9361 gccgatgata aacatcagga ataccacgcc gagttccgaa aagtggagga tctcatcgac
     9421 gtcgctaata aaccccagcc cccacgggcc aatcgcaatc cccgccagca aatatcccaa
     9481 cactgcacca atacccagcc gcgatgccag cggcaccgca gccaccgccg cgaagagaaa
     9541 cagcactcct gcgagtaaaa aatcagaacc ttccatcagc ggcctcctgg agacaacgga
     9601 tttgccagcc agtcaccgta ggctctggcg tggctcgcca gctcctgtgc gctttgccgt
     9661 ctcgcccagt aaataatgat ggggcttaac caatgcatcc ggcacatgcc cgccgccagt
     9721 tcaaaggggc gcagaacatc gctcatcgga tagcgattca acgcgtcata acggtaagca
     9781 ctttccggct ccccggtggt aatcacgcta cgccagtact ttcccgccag ttggtttccc
     9841 cccggtccgc tggcaaaacc acggcttaat acccggtcca gccactcttt cagtagcgcc
     9901 gggcagctat aggtataaag aggatgctga aagacaatca cctcgtgctc gcgcagtaat
     9961 gcctgctcac gagggatatc aataaaaaag tcgggatagt gcgcgtaaag gtcgtgcacg
    10021 gtaacattgc tgagctgcgt ggccggttta agcagaaccc ggtttgccac cgagtcctga
    10081 gattccggat gggcatacag cagcaaaact ttcgctggct gagacatcat ccccctcccg
    10141 gtatggtttt tgtgtatagt cgctgttttg ggctaccatt gcgcccggtg cggcagctcg
    10201 cccatacatt acattatcat aatgataagt taacatagtc tgaacatacg gcgccttatg
    10261 attgtttttt cctcgttaca aattcgtcgc ggcgtgcgcg tcctgctgga taatgccacc
    10321 gccaccatca accctgggca gaaagtcggc ctggtgggta aaaacggctg tggtaaatct
    10381 accctgctgg cattgctgaa aaatgaaatc agcgccgacg gcggcagcta cacctttccg
    10441 ggaagctggc aactggcgtg ggtgaatcag gaaacaccgg cgttaccgca agcggcgctg
    10501 gaatatgtca ttgacggcga ccgtgaatat cgtcaactgg aagcgcagct acacgacgcc
    10561 aacgaacgta acgacgggca agccattgca accattcatg gcaagctgga tgctattgac
    10621 gcatggagta ttcgctcccg tgccgccagc ctgctgcacg gcctcggttt cagcaatgaa
    10681 caactggagc gcccggtaag tgatttctcc ggtggctggc gtatgcgtct taaccttgcc
    10741 caggcgctga tttgccgttc agacttgctg ctgctcgacg aaccgactaa ccacctcgat
    10801 ctcgatgctg ttatctggct ggaaaaatgg ctgaagagct atcagggcac gctgatcctg
    10861 atctctcacg accgcgactt cctcgatccg atcgttgata aaattattca tatcgaacaa
    10921 caaagcatgt tcgagtacac cggcaactac agttcgtttg aagtacagcg cgccacccgt
    10981 ctggcgcagc aacaagcgat gtacgaaagc cagcaggaac gcgtagcgca tctgcaaagt
    11041 tatatcgacc gtttccgtgc caaagccacc aaagcgaagc aggcccagag ccgtattaag
    11101 atgctggagc gtatggagct gattgccccg gcgcacgtcg acaacccgtt ccgctttagc
    11161 ttccgcgcgc cggaaagcct gcctaatccg ttattaaaga tggaaaaagt cagcgcaggc
    11221 tatggtgatc gcattattct cgactcgatt aaactgaatc tggtccccgg ctcgcgcatt
    11281 ggtctgctag gccgcaacgg cgcgggtaaa tcgacattaa tcaaactgtt agccggtgaa
    11341 cttgcgccag tcagcggtga aattggtctg gcgaaaggga tcaagctcgg ctacttcgcc
    11401 cagcatcaac ttgaatacct gcgcgccgac gaatcgccta ttcaacatct ggcacgttta
    11461 gcgccgcagg agctggaaca aaaactgcgt gattacctcg gcggctttgg tttccagggc
    11521 gataaagtaa ccgaagaaac gcgccgcttt tccggtgggg aaaaagcccg cctggtgctg
    11581 gcattaattg tctggcagcg tccgaatctg ctgctgctcg acgaaccgac taaccacctt
    11641 gacctcgaca tgcgtcaggc actcactgaa gcattaatcg agttcgaagg cgcgctggtt
    11701 gtcgtctcgc acgaccgtca tttgctgcgt tccaccactg acgatctcta cctggttcac
    11761 gatcgtaaag tcgaaccgtt cgacggcgat ctggaagatt atcaacagtg gttgagcgac
    11821 gtacaaaagc aggaaaacca ggccgacgaa gcgccaaaag agaacgcgaa cagcgcccag
    11881 gcacgtaaag atcagaagcg tcgggaagcg gagctgcgtg cgcaaaccca gccactgcgt
    11941 aaagagattg cccgtctgga aaaagagatg gagaagctga acgcgcaact ggcgcaggcg
    12001 gaagagaaac tcggcgacag cgaactgtat gaccagagcc gtaaagcgga gttgaccgcc
    12061 tgcctgcaac agcaagccag cgccaaatcc ggcctggaag agtgcgaaat ggcgtggctg
    12121 gaagcccagg agcagcttga gcagatgctg ctggaaggcc aaagcaactg atggcgcaga
    12181 taacgacgac cgatgccaat gaatttagca gcagtgctga attcacccct atgcgcggct
    12241 ttagcaattg tcatctgcaa accatgctgc cgcgtctgtt tcgtcgcaag gtgaaattca
    12301 ccccgcactg gcatcgactg gagttgcccg acggcgattt tgtcgatctc gcatggagtg
    12361 aagatcctgc acaggcgcgg cataaaccgc gtttagtggt atttcacggg ctggaaggca
    12421 gtctcaatag cccttacgct cacggtctgg ttgaggcggc gcaaaagcgc ggctggctgg
    12481 gcgtggtgat gcattttcgc ggatgcagcg gtgaaccaaa ccgtatgcac cgcatttacc
    12541 attcgggcga aaccgaagac gccagttggt ttttacgctg gctgcaacgc gagtttggtc
    12601 atgcgccaac ggctgccgtc ggctattcgc tcggcggtaa tatgctggcc tgtttgctgg
    12661 caaaagaagg taatgatctc ccgattgatg cggcggtgat tgtctctgcg ccgtttatgc
    12721 tggaggcctg tagttaccat atggaaaagg gcttttcccg catttatcag cgttacttgc
    12781 tgaacctgtt aaaagccaat gccgcgcgca agctggcagc ctaccccgga acactgccga
    12841 ttaatctcgc gcagttaaaa tcggtacgtc gcatccgtga atttgacgat ctcatcaccg
    12901 ccagaattca cggctacgct gacgctatcg actattatcg tcagtgtagc gccatgccga
    12961 tgctcaatcg gatagccaaa ccgacactga ttattcacgc caaagacgat ccgtttatgg
    13021 atcatcaggt gttcccgaaa ccggaaagtc tccccccgca ggtggagtat cagctgactg
    13081 aacatggcgg tcatgttggc tttattggcg gtacattact tcatccgcaa atgtggctgg
    13141 agtcacgcat tcctgactgg ttaacaacgt atctggaggc gaaatcatgt tgattccgtg
    13201 gcaagacctc tcccccgaaa cgctggaaaa tttaattgaa agctttgtgt tacgtgaagg
    13261 caccgattat ggtgaacatg agcgtacact tgaacagaaa gtcgccgacg tcaaacgcca
    13321 gctacagtgc ggagaagcgg tgctggtatg gtcggagctg cacgaaacgg tcaatatcat
    13381 gccgcgcagt cagtttcgcg aataacctgc aaccgtcggc ggacgagata ataatgatag
    13441 ctaagtcatg gagttactat gtctgccaaa catccggtca ttgcggtaac aggatccagc
    13501 ggcgcgggga ccaccaccac cagcctcgcg tttcgtaaaa tattcgcgca gttaaatctg
    13561 catgcagccg aggtggaagg cgacagtttt caccgttata cccgcccgga aatggacatg
    13621 gcgatccgca aagcgcgcga cgccgggcgg catatcagct acttcggccc cgaagcgaac
    13681 gacttcggcc ttctggaaca aaccttcatt gaatacggtc agagtggcaa agggaaatct
    13741 cgcaaatatc tgcataccta cgatgaagcc gtaccgtgga atcaggtacc ggggacattc
    13801 accccctggc aatctttacc ggaacccact gatgtactgt tttatgaagg tttacacggc
    13861 ggcgtagtca cgccacagca taacgttgcg cagcatgtgg acttactggt cggcgtggtg
    13921 cctatcgtta accttgagtg gattcaaaaa ctgatccgcg acaccagcga gcgcgggcac
    13981 tcacgagaag cagtgatgga ctcggtagtg cgttcaatgg aagactatat caactacatc
    14041 acaccgcagt tttcccgcac ccatcttaac ttccagcgcg ttcccaccgt cgacacctca
    14101 aacccgttcg cggcaaaagg tatcccgtca ctggatgaaa gctttgtggt gatccatttt
    14161 cgtaatctgg aagggatcga tttcccctgg ctgctggcga tgttgcaagg ctcattcatt
    14221 tcccacatca atacgttagt ggtgcctggc ggcaaaatgg gtctggcaat ggaattaatt
    14281 atgctgccgc tggtgcaacg attgatggaa ggaaagaaaa tcgagtaacg ctgctattac
    14341 gccggataaa atactatccg gcttcacaac gggatagtta aatcacgcgg caaccacttc
    14401 atacgagtga gtaatattca ccgctttttc cagcatcagc gccactgagc aatatttctc
    14461 ggcagaaaga tcaaccgcac gcgcaaccgc tgcgtctttc agatcgcgac cggtgacgat
    14521 aaaatgtaga ttaatgtgcg taaacaggcg tggtgcctct tcgcggcgct cagaggtcaa
    14581 ttttacttca caatcgacca catcctgacg ccctttttgc aggatagaaa ccacatcgat
    14641 ggcactgcaa ccacccgccg ccatcaacac catttccatc gggcttggtg ctttatcgcc
    14701 tgagttgccg tccattaaaa tctggtgacc agaggcggat tcgcccagaa aagttaaccc
    14761 ttcgacccac ttcactcgcg cttgcatttt tgctactcca ctgcgtcaat tttcctgaca
    14821 gagtacgcgt actaaccaaa tcgcgcaacg gaaggcgacc tgggtcatgc tgaagcgaga
    14881 caccaggaga cacaaagcga aagctatgct aaaacagtca ggatgctaca gtaatatatt
    14941 gatgtactgc atgtatgcaa aggacgccac attaccgtgc agtacagttg atagcccctt
    15001 cccaggtagc gggaagcata tttcggcaat ccagagacag cggcgttatc tggctctgga
    15061 gaaagcttat aacagaggat aaccgcgcat ggtgcttggc aaaccgcaaa cagacccgac
    15121 tctcgaatgg ttcttgtctc attgccacat tcataagtac ccatccaaga gcacgcttat
    15181 tcaccagggt gaaaaagcgg aaacgctgta ctacatcgtt aaaggctctg tggcagtgct
    15241 gatcaaagac gaagagggta aagaaatgat cctctcctat ctgaatcagg gtgattttat
    15301 tggcgaactg ggcctgtttg aagagggcca ggaacgtagc gcatgggtac gtgcgaaaac
    15361 cgcctgtgaa gtggctgaaa tttcgtacaa aaaatttcgc caattgattc aggtaaaccc
    15421 ggacattctg atgcgtctgt ctgcacagat ggcgcgtcgt ctgcaagtca cttcagagaa
    15481 agtgggcaac ctggcgttcc tcgacgtgac gggccgcatt gcacagactc tgctgaatct
    15541 ggcaaaacaa ccggatgcta tgactcaccc ggacggtatg caaatcaaaa ttacccgtca
    15601 ggaaatcggt cagattgtcg gctgttctcg tgaaaccgtg ggacgcattc tgaagatgct
    15661 ggaagatcag aacctgatct ccgcacacgg taaaaccatc gtcgtttacg gcactcgtta
    15721 atcccgtcag agtggcgcgt tacctggtag cgcgccattt tgtttccccc gatgtggcgc
    15781 agactgattt atcaccccga tatcaactat gcacttcgac aaacgctggt gctatgtttg
    15841 cccgtggccg ttgggttaat gcttggcgaa ttacgattcg gcctgctctt ctccctcgtt
    15901 cctgcctgtt gcaatattgc gggccttgat acgcctcata aacgtttttt caaacgctta
    15961 atcattggtg cgtcgctgtt tgccacctgt agcttgctga cacaggtgct actggcaaaa
    16021 gatgttcccc tgcccttttt gctgaccgga ttaacgctgg tacttggcgt cactgctgag
    16081 ctggggccat tgcacgcaaa attgcttccc gcatcgctgc tcgccgccat ttttaccctc
    16141 agtctggcgg gatacatgcc ggtctgggaa ccgttgctca tctatgcgct gggcactttg
    16201 tggtacggat tgtttaactg gttttggttc tggatctggc gcgaacaacc gctacgcgag
    16261 tcactaagtc tgctgtaccg tgaactggca gattactgtg aagccaaata cagcctgctt
    16321 acccagcaca ccgaccctga aaaagcgctg ccgccgctgc tggtgcgcca gcaaaaagcg
    16381 gtcgatctaa ttacccagtg ctatcagcaa atgcatatgc tttccgcgca aaataatacc
    16441 gattacaagc ggatgctgcg tattttccag gaggcgctgg atttgcagga acatatttcg
    16501 gtcagtttgc atcagccgga agaggtgcaa aagctggtcg agcgtagcca tgcggaagaa
    16561 gttatccgct ggaatgcgca aaccgtcgcc gctcgcctgc gcgtgctggc tgatgacatt
    16621 ctttaccatc gcctgccaac gcgttttacg atggaaaagc aaattggcgc actggaaaaa
    16681 atcgcccgcc aacacccgga taatccggtt gggcaattct gctactggca tttcagccgc
    16741 attgctcgtg tgctgcgcac ccaaaaaccg ctctatgccc gtgacttact ggccgataaa
    16801 cagcggcgaa tgccattact tccggcgctg aaaagttatc tgtcactaaa gtctccagcg
    16861 ctacgcaatg ccggacgact cagtgtgatg ttaagcgttg ccagcctgat gggcaccgcg
    16921 ctgcatctgc cgaagtcgta ctggatcctg atgacagtat tgctggtgac gcaaaatggc
    16981 tatggcgcaa cccgtctgag gattgtaaat cgctccgtag gaaccgtggt cgggttaatc
    17041 attgcaggcg tggcgctaca ctttaaaatt cccgaaggtt acaccctgac cttgatgctg
    17101 attaccaccc tcgccagcta cctgatattg cgcaaaaact acggctgggc gacggtcggt
    17161 tttactatta ccgcagtgta taccctgcaa ctgttgtggc tgaacggtga gcagtacatc
    17221 cttccgcgtc ttatcgatac cattattggt tgtttaattg ctttcggcgg tactgtctgg
    17281 ctgtggccgc agtggcagag cgggttattg cgtaagaacg cccatgacgc tttagaagcc
    17341 tatcaggacg cgattcgctt gattctgagc gaggacccgc aacctactcc actggcctgg
    17401 cagcgaatgc gggtaaatca ggcgcataat actctgtata actcattgaa tcaggcgatg
    17461 caggaaccgg cgtttaacag ccattatctg gcagatatga aactgtgggt aacgcacagc
    17521 cagtttattg ttgagcatat taatgccatg accacgctgg cgcgggaaca ccgggcattg
    17581 ccaccggaac tggcacaaga gtatttacag tcttgtgaaa tcgccattca gcgttgtcag
    17641 cagcgactgg agtatgacga accgggtagt tctggcgatg ccaatatcat ggatgcgccg
    17701 gagatgcagc cgcacgaagg cgcggcaggt acgctggagc agcatttaca acgggttatt
    17761 ggccatctga acaccatgca caccatttcg tcgatggcat ggcggcagcg accgcatcac
    17821 gggatttggc tgagtcgcaa gttgcgggat tcgaaggcgt aatacaggct aaaccatcgg
    17881 atgaggtgtt cacgccgcat ccgacatctt ttgcctgatg cgtcttattt acgccccaac
    17941 caccttcgcc accgcgtggg cgaaacgatg catcccttca tcgatatccg catcttccac
    18001 caccagcgac ggcgcaaaac gcatcacatc aggcccggca ttcagcacca ttacgccttc
    18061 ctctgcaccc gcatacagga aatcacgcgc ccgacctttg tactgtggtt tcagctctgc
    18121 gccaatcaac agccccatac cgcgaatatc gctaaacaca tcgtactgct gatcgatctt
    18181 ctgcaaatgg tcaacaaaac gctggcgttt cgcctgaata ccttccagca cttcaggggt
    18241 attgatgata tcaaacgccg cgcccgctac tgcacaggcc agaggattac cgccgtaggt
    18301 ggaaccgtga gaaccaggat gaaacgcgga agcaatttcc gccgtggtca gcatggcgct
    18361 aatcgggaag ccgccgccta acgctttcgc agaggtcaga atatccggcg tcacgccgta
    18421 gtgcatgtaa gcaaacaaat cgccggtccg ccccatcccg cactgtactt catcaaacac
    18481 caataatgcc tgatgttgat cgcacagctc gcgcaagccc tgcaaaaact ctggcgtcgc
    18541 tgccgtcaca ccgccctcac cctggatcgg ctcaaccacc accgcacagg tgtgatcatc
    18601 catcaccgct ttcactgcgt ggagatcgtt aaagggaacg tggatgatgt ctgccggttt
    18661 tggcccaaag ccatcggaat attttggttg cccgcccacc gagacggtaa acagcgagcg
    18721 accatgaaaa gcgttatgga aggcgataat tttggttttg aacgggctat gacgcacaca
    18781 ggcgtaatgg cgtgccagtt taaaggcggt ttcgttagct tccgtgccgg agttcataaa
    18841 caccacgcgt tcggcaaacg ttgcctcaat cagtttacga ccaagacgca gcgccggttc
    18901 attggtgaaa acgttactga tatgccacag agtttcgccc tgggttttta acgcgttcac
    18961 cagcgcagga tggcaatggc ccaacgccgt aactgcaatg ccacccgcga aatcgacata
    19021 ctccttgcct tgctgatccc agattcggct gccctgacct tttaccggaa taaactctgc
    19081 cggagcataa atcggcagga tcacttcatc gaaagtcgcg cgtgtaattg ctgtttgttc
    19141 aattgccatc tcatgatcac cctgttacgc ataaaccaat gtgaaattat aatcacaaaa
    19201 tatgcataaa aaatcactta atagcaatca gaaatcagcg atgcaggaaa ttagccagca
    19261 gttgatgtcc ttgttcgcta agaatacttt ctggatggaa ttgcacacct tccagatccc
    19321 actggcgatg gcgaatcccc ataatctcgc gggtttcgct ccaggccgtc acttcaaagc
    19381 actccggtaa tgagtcaggt tccaccacca gcgaatggta gcgtgtcacg gtgagtggat
    19441 ttgccagccc cttaaatacg cccacaccat tatgtgtaat cggcgaggtt ttgccgtgca
    19501 tgacctttgc ggcgcgcaca actttgccgc caaatgcctg tgccattgcc tggtgaccga
    19561 ggcagacgcc aagaatcggc aaacgcccgg catagtgacg aataacgtca agggagatcc
    19621 cggcttcatc tggcgtacag gggccgggtg agatgacaat tttttgtggt ttaagggcgt
    19681 cgatatccgc caacgtcaac gcatcgttgc gcttaaccaa cacatccgcc cccagttcac
    19741 aaaagtactg gtagaggttc caggtaaaag aatcgtagtt atcaataagc aggatcatgg
    19801 cggctccggt acgaaagaac cgcgctattt tactcagatt ccccgacttc gcttaccact
    19861 ttgctaaata tcatctgcag cgcggtgaga tcccccattg ccccattctg atttgcctga
    19921 ttccaggcct ctttttcgat accctgccag ctcagttgat atccggcatg aatcgccagt
    19981 tgctcgaaga agatccgctg tgccaaacca cttcccaccc ggaagggatg cagcacgttg
    20041 atttcacaat agtaatgcgc caaccgctcg acgaatttat ctttctcaag gctagtcaga
    20101 tacttctctt cctccaaatc ctgcatcagg gcattgccct ctttttcgag ataggcaaag
    20161 tggcagaacg gcgtatctcc ctgataaata tcaacttcac gcagttgccc tgcccagtcg
    20221 aaaatatcct gatacagctg gcgatggata gtgcgcaaat gcggtaaacc gcgcaccagc
    20281 ggaccaagct caatggtcgc agcacgcagc gccgtcattt cgtaagcggc ctgttccagc
    20341 cgctgctgct ggcggatgtt gagcttgtta cgcatgatat cgagacccgg gtagagatat
    20401 ggatcgcgcc cttcgccgaa tttatcgctc atagtgcctc ctcagcgctt caaggcgcgc
    20461 taaagcctct gcggcagtaa gagtgactaa aggcatctcg acgccttcaa gacggcgact
    20521 ggcctggaaa ttacgattac gctgaagctc ccagagacgg gacttttgct tatcggtgag
    20581 tttcttcact tgatgcctcc ctgaacgtgt ccatttgcca caagtataag cggcaaatcc
    20641 gggttacgcc gggagagcag aagcgcgggc aagattgccc gcgcgagaaa tcattacggc
    20701 aggactttag cggaaaggat aactaccggt tttgacggca cattctggta cggaccaacg
    20761 tcatgagtcg gcacctggga aatcttatcg gcaacgtcca tgcctttcac cactttacca
    20821 aataccgcgt aaccgaaatc acgctgaccg tggtcaagga aggcgttatc ggcaacgttg
    20881 ataaagaact ggctggtggc gctgtctttg tcagcggtac gcgccatcgc gatggtgcca
    20941 cgcgtgttgc gcaggccgtt atcagcttca tttttgattg gcgggtttgg ttttttctgc
    21001 tgcatctgct cggtgaaacc gccgccctga atcataaagc caggaatgac gcggtgaaag
    21061 gtcgtgttgt tataaaaacc actgttcaca taatcgacaa agttttgcac agacactggc
    21121 gctttttgtt tatccagctc cagttcgatg ttaccagctg aggttgtcaa caatacgtgc
    21181 gggtcccctt tcgctgccat tgctgcggga gaaagagcag aaagagcgaa aacagcagcc
    21241 atcgccgcca gggtcgattt gaacatgaga tttccttaac aaacagagca gaaaaaaagc
    21301 aagtgaagag attctaaata gccttcaaga acaaggccat ccctttacct aattttacgt
    21361 atctgaaata tatgtaacct gaggataact ttttattccg tgatcaaaat cacctcttaa
    21421 aatgcaattt agcaaccgat tgcaataaaa catttaatca gatcacaaaa tcgcctaaaa
    21481 tcgcccgctc gcggcatagc ccgctatgcc attttatttt tgaacgcagg ccaatcatga
    21541 ctaacagcaa tcgcatcaag ctcacatgga ttagctttct ttcctacgca ctgaccggtg
    21601 cgttggttat tgtcaccggg atggtgatgg gaaatatcgc cgattatttc aatctgcctg
    21661 tttccagcat gagtaacacc ttcaccttcc tcaatgccgg cattttaatc tctatcttcc
    21721 tcaacgcctg gctgatggaa atcgtcccgt tgaaaacgca gttacgtttt ggctttctcc
    21781 tgatggtgct ggcggttgcc ggtttgatgt tcagccacag cctggcgctg ttctcggcgg
    21841 cgatgttcat tctcggggtg gtcagcggca tcaccatgtc gattggtaca ttcctggtaa
    21901 cacaaatgta tgaaggacgt cagcgcggtt cacgcctgtt atttaccgac tccttcttca
    21961 gtatggccgg gatgattttc ccaatgatcg ccgcatttct gctggcgcgt agcattgagt
    22021 ggtactgggt ttatgcctgc atcgggctgg tgtatgtcgc tatttttatt ctgaccttcg
    22081 gctgtgagtt cccggcgctg ggcaaacatg cgcccaaaac ggatgctccg gtagagaaag
    22141 aaaagtgggg gatcggcgta ctgtttctct ctgttgcggc gctgtgctac atcctcggtc
    22201 agttaggttt tatctcctgg gtgccggaat atgccaaagg cctgggcatg agcctgaacg
    22261 acgcaggcac gctggtaagt aacttctgga tgtcgtacat ggtcggcatg tgggcattca
    22321 gctttattct tcgcttcttt gatttgcaac gtatcctgac cgtactggct ggtctggctg
    22381 cgattctgat gtacgtcttt aacaccggaa caccggcaca tatggcgtgg tcaattctcg
    22441 ccctgggctt cttctccagc gcgatctata ccaccatcat caccctgggt tcacagcaga
    22501 ccaaagtacc gtcgccaaaa ctggttaact ttgtcctgac ctgcgggacc atcggtacta
    22561 tgttgacctt tgtggttacc ggcccgattg ttgaacatag tggtccgcag gcggcgctgc
    22621 tgacggcaaa cggtctgtac gctgtcgtct ttgtgatgtg cttcctgtta ggtttcgtca
    22681 gccgtcaccg tcagcataac accctgacct ctcattaatt gttcatgccg ggcggcacta
    22741 tcgtcgtccg gccttttcct ctcttaccct gctacgtgca tctatttcta taaacccgtt
    22801 caatttgtct gttttttgca caaacatgaa atatcagaca attccgtgac ttaagaaaat
    22861 ttatacaaat cagcaatata ccccttaagg agtatataaa ggtgaatttg atttacatca
    22921 ataagcgggg ttgctgaatc gttaaggtag gcggtaatag aaaagaaatc gaggcaaaaa
    22981 tgagcaaagt cagactcgca attatcggta acggtatggt cggccatcgc tttatcgaag
    23041 atcttcttga taaatctgat gcggccaact ttgatattac cgttttctgt gaagaaccgc
    23101 gcatcgctta tgaccgcgta cacctctcgt cttacttctc tcaccacacc gccgaagagc
    23161 tgtcgctggt gcgcgaaggc ttctacgaga aacacggcat caaagttctg gtcggcgaac
    23221 gcgctatcac catcaaccgt caggagaagg tgattcactc cagcgccgga cgtaccgttt
    23281 tttatgacaa gctgatcatg gcgaccggtt cctacccgtg gatcccgcca atcaaaggtt
    23341 ctgatactca ggactgcttt gtctatcgca ctattgaaga cctcaacgcc attgaatcct
    23401 gcgcccgtcg cagcaaacgc ggtgccgttg ttggtggcgg cctgttaggt ctggaagccg
    23461 ctggcgcgct gaaaaactta ggcattgaaa cccacgttat cgaatttgcc cctatgctga
    23521 tggcggaaca gcttgatcag atgggcggcg agcagctgcg tcgcaaaatc gaaagtatgg
    23581 gcgtgcgcgt tcacaccagc aaaaacactc ttgagattgt gcaggaaggt gttgaagcgc
    23641 gtaaaaccat gcgttttgcc gacggcagcg aactggaagt cgactttatc gtcttctcta
    23701 ccggtatccg tccgcgcgat aagctggcaa cccagtgtgg tctggacgtt gctccgcgtg
    23761 ggggtatcgt cataaatgat tcctgccaga cttccgatcc ggatatctac gccatcggtg
    23821 aatgcgcaag ctggaacaac cgtgtatttg gtctggtagc accaggctac aaaatggcgc
    23881 aggtcgccgt tgaccatatt cttggtagcg aaaacgcctt tgaaggtgca gaccttagcg
    23941 ccaagctgaa actgctgggc gtagacgtag gcggtattgg tgatgcgcac ggtcgcacgc
    24001 ctggcgcacg tagctacgtt tacctcgacg aaagcaaaga gatctacaaa cgcctgattg
    24061 tcagcgaaga caacaaaacc ctgctcggtg cggtactggt gggcgatacc agcgactacg
    24121 gcaacctgct gcaactggtg ctgaacgcta tcgaactgcc ggaaaacccg gattctctga
    24181 ttctgccggc gcactcgggt agcggtaagc cgtctatcgg tgttgataaa ctgccggaca
    24241 gcgcgcaaat ctgctcctgc ttcgacgtca ccaaaggcga cctgattgct gctatcaaca
    24301 aaggctgcca caccgttgcg gcgctgaaag ccgaaaccaa agccggtacg ggctgcggcg
    24361 gttgtattcc gctggtcact caggtactga acgcggaact ggcgaaacag ggtatcgaag
    24421 ttaacaacaa cctgtgtgaa cactttgctt attcgcgtca ggaactgttc catttgatcc
    24481 gcgttgaagg cattaaaacc ttcgaagaac tgctggcgaa acacggcaaa ggttacggtt
    24541 gtgaagtttg taaaccgacc gtcggttcgc tgctggcctc ctgctggaac gaatacattc
    24601 tgaagccgga acatactccg ctgcaggatt ctaacgacaa cttcctcgct aacatccaaa
    24661 aagacggcac ctactcggtg atcccgcgtt ctccgggtgg tgaaatcacc ccggaagggc
    24721 tgatggcagt gggtcgtatc gcgcgtgaat ttaatctcta caccaaaatc accggctccc
    24781 agcgcctggc gatgtttggc gcacagaaag acgatctgcc ggagatctgg cgtcagctga
    24841 ttgaagcagg tttcgaaacc ggtcatgcct atgcgaaagc actgcgtatg gcgaaaacct
    24901 gcgtgggtag cacctggtgc cgctatggcg ttggcgacag cgtcggcctc ggcgtggaac
    24961 tggaaaatcg ctacaaaggc atccgtacgc cgcacaaaat gaagttcggt gtctccggct
    25021 gtacccgtga atgttcagaa gctcagggta aagacgtggg tattatcgcc actgaaaaag
    25081 gctggaacct gtatgtttgc ggtaacggcg gcatgaaacc gcgtcatgcg gatctgctgg
    25141 cagcggatat cgatcgcgaa acgctgatta aatatcttga ccgcttcatg atgttctaca
    25201 tccgtactgc cgacaaactg acgcgtaccg cgccgtggtt agaaaacctc gaaggtggca
    25261 tcgattacct gaaagcggtg atcattgacg acaaactggg gctgaacgca catctggaag
    25321 aagagatggc gcgcctgcgt gaagcggtag tgtgtgagtg gactgaaacg gtcaataccc
    25381 cgtctgcgca gactcgcttc aaacacttca tcaacagcga caagcgtgac ccgaacgtgc
    25441 agatggtgcc agagcgcgaa cagcaccgtc cggcaacgcc gtatgaacgt atcccggtaa
    25501 ctctggtgga ggacaacgca tgagccagtg gaaagacatc tgcaaaatcg atgacatcct
    25561 gcctgaaacc ggcgtctgcg cgctgttagg tgacgaacaa gtagcaattt tccgcccgta
    25621 tcacagcgac caggtgtttg cgatcagcaa catcgatccg ttcttcgagt ccagcgtgct
    25681 gtcacgcggg ctgattgcgg aacaccaggg cgagctgtgg gtcgccagcc cgctgaaaaa
    25741 gcagcgtttt cgcttaagcg acggcttgtg catggaagac gaacagtttt ccgtcaagca
    25801 ttacgacgcg cgagtgaaag acggcgtggt gcagctgcgc ggttaatatt ttaacgggag
    25861 gcgcaatgcc tccccctttt tgcatggtcc tgtaataacc ttcggtatat tgcaggacat
    25921 tttttaaact ttttgtttta ttttttgttt ttatttttta aaggataatc aaatgtttac
    25981 agacactatt actaagtgtg cggctaacgc tgcgcgcatt gcacgcctgt cggcaaataa
    26041 cccgctcggc ttttgggtca gctccgccat ggcgggcgcg tatgtgggtc ttgggatcat
    26101 cctgattttc acgctcggta atttgctcga cccgtccgta cgtcctctgg tgatgggtgc
    26161 gacctttggt atcgccttaa cgctggtgat tatcgccggt tctgaactgt tcaccggaca
    26221 caccatgttc ctcacctttg gagtgaaagc gggcactatc agccacgggc aaatgtgggc
    26281 aatcctgccg caaacctggc tgggtaacct ggtcggttcc gtctttgttg ccatgctcta
    26341 tagctggggt ggcggtagcc tgctgccggt cgataccagc atcgttcact ccgtcgcgct
    26401 ggctaaaacc actgcaccgg caatggtact gttcttcaaa ggtgcattgt gtaactggct
    26461 ggtttgcctg gcaatctgga tggcgctgcg cactgaaggg gcggcgaaat ttatcgctat
    26521 ctggtggtgt ttgctggcat ttatcgcgtc cggctacgag cactccatcg ctaacatgac
    26581 gctgttcgcc ctctcctggt tcggcaacca cagcgaagcc tacacgctgg cgggtattgg
    26641 tcataacctg ctgtgggtga cgctgggtaa tactttatcg ggtgccgtat tcatgggatt
    26701 gggttattgg tatgctacgc cgaaagcgaa tcgtccggtt gcggacaaat ttaatcaaac
    26761 tgaaacggct gccggttaat tattaagggg tttttacgtg gatcatttgc ctatattttg
    26821 ccaattacgc gatcgcgact gtctgattgt cggcggtggt gatgtcgcgg aacgcaaagc
    26881 aaggttgctg ttagacgcag gcgctcgctt aacggtgaat gcattagcgt ttattccaca
    26941 gttcaccgca tgggcagatg caggcatgtt aaccctcgtc gaagggccat ttgatgaaag
    27001 ccttctcgac acctgctggc tggcgattgc agcgacggat gatgacgcgc ttaaccagcg
    27061 cgtcagcgaa gccgctgaag ctcgtcgcat cttctgtaac gtggtcgatg cgccgaaagc
    27121 cgccagcttt attatgccgt cgattattga ccgctcgccg ctcatggtag cggtctcctc
    27181 tggcggcacc tctccggttc tggcgcgcct gttgcgcgaa aaactcgaat cactgctgcc
    27241 gttacatctg ggccaggtag cgaaatacgc cgggcaatta cgcgggcgag tgaaacaaca
    27301 gttcgccacg atgggtgagc gtcgccgttt ctgggagaaa ttgttcgtta atgaccgcct
    27361 ggcgcagtcg ctggccaaca acgatcagaa agccattact gaaaccaccg aacagttaat
    27421 caatgaaccg ctcgatcatc gcggtgaagt ggtgctggtt ggtgcaggtc cgggcgatgc
    27481 cgggctgctg acactgaaag gtctgcaaca aattcagcag gcagatgtgg tggtctacga
    27541 ccgtctggtt tctgacgata ttatgaatct ggtacgccgc gatgctgatc gcgttttcgt
    27601 cggcaaacgc gcgggatacc actgcgtacc gcaggaagag attaaccaga tcctgctgcg
    27661 ggaagcgcaa aaaggcaaac gcgtggtgcg gctgaaaggc ggcgatccgt ttatttttgg
    27721 ccgtggtggc gaagagctgg aaacactgtg caacgcgggt attccattct cggtggttcc
    27781 gggtattacc gcagcctctg gttgctctgc ctattcgggt attccgctca cgcatcgcga
    27841 ttatgcccag agcgtacgct taattactgg acacttaaaa accggtggcg agctggactg
    27901 ggaaaacctg gcggcagaaa aacagacgct ggtgttttat atggggctga atcaggctgc
    27961 aactattcag caaaagctga ttgaatacgg tatgcctggt gaaatgccgg tagcaattgt
    28021 cgaaaacgga acggcagtca cgcagcgcgt gattgacggt acgctcacgc agctgggcga
    28081 actggcgcag caaatgaaca gtccatcgct aattattatt ggtcgggttg ttggcctgcg
    28141 cgataaattg aactggttct ctaaccatta atttaaccag ggccagagaa gtctggcctt
    28201 tttcgtttac aactctcttt atatattcag cagataaaaa tataacccct taataaatat
    28261 tgtctgctaa tgggttatat tcaccgttac agcaacttac aaaattaagc caatcgctca
    28321 ctgacgctaa ttgaatattc ctgtatcgtt cttaat
//
LOCUS       DAHHTG010000057        28298 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.057, whole genome shotgun sequence.
ACCESSION   DAHHTG010000057 DAHHTG010000000
VERSION     DAHHTG010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28298)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28298)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28298
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.057"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            345..1484
                     /gene="wza"
                     /locus_tag="NBI74_002385"
     CDS             345..1484
                     /gene="wza"
                     /locus_tag="NBI74_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein Wza"
                     /protein_id="HCD1932709.1"
                     /translation="MMKSKMKLMPLLVSVTLISGCTVLPGSNMSTMGKDVIKQQDADF
                     DLDKMVNVYPLTPRLIDQLRPRPNVARPNMTLESEIANYQYRVGPGDVLNVTVWDHPE
                     LTTPAGQYRSSSDTGNWVQPDGTMFYPYIGKVHVVGKTLAEIRSDITGRLATYIADPQ
                     VDVNIAAFRSQKAYISGQVNKSGQQAITNVPLTILDAINAAGGLTDTADWRNVVLTHN
                     GREERISLQALMQNGDLNQNRLLYPGDILYVPRNDDLKVFVMGEVKKQSTLKMDFSGM
                     TLTEALGNAEGIDMTTSNASGIFVIRPLKGEGGRNGKIANIYQLDMSDATSLVMATEF
                     RLQPYDVVYVTTAPVSRWNRLINQLLPTISGVRYMTDTASDIHNW"
     gene            1490..1933
                     /gene="wzb"
                     /locus_tag="NBI74_002386"
     CDS             1490..1933
                     /gene="wzb"
                     /locus_tag="NBI74_002386"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase Wzb"
                     /protein_id="HCD1932710.1"
                     /translation="MFNNILVVCVGNICRSPTAERLLQRYHPELKVESAGLGALVGKG
                     ADPTAISVAAEHQLSLEGHCARQISRRLCRNYDLILTMEKRHIERLCEMAPEMRGKVM
                     LFGHWDNECEIPDPYRKSRETFTAVYALLERSARQWAQALNAEQV"
     gene            1936..4098
                     /gene="wzc"
                     /locus_tag="NBI74_002387"
     CDS             1936..4098
                     /gene="wzc"
                     /locus_tag="NBI74_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase Wzc"
                     /protein_id="HCD1932711.1"
                     /translation="MTEKVKQHAAPVTGSDEIDIGRLVGTVIEARWWVIGITAVFALC
                     AVVYTFFATPIYSADALVQIEQSSGNSLVQDIGSALANKPPASDAEIQLIRSRLVLGK
                     TVDDLDLDIAVSKNTFPIFGAGWERLMGRQNETVKVTTFNRPKEMADQVFTLNVLDNK
                     NYTLSSDGGFSARGQAGQMLKKEGVTLMVEAIHASPGSEFTVTKYSTLGMINQLQNSL
                     TVTENGKDAGVLSLTYTGEDREQIRDILNSIARNYQEQNIERKSAEASKSLAFLAQQL
                     PEVRSRLDVAENKLNAFRQDKDSVDLPLEAKAVLDSMVNIDAQLNELTFKEAEISKLY
                     TKVHPAYRTLLEKRQALEDEKAKLNGRVTAMPKTQQEIVRLTRDVESGQQVYMQLLNK
                     EQELKITEASTVGDVRIVDPAITQPGVLKPKKGLIILGAIILGLMLSIVGVLLRSLFN
                     RGIESPQVLEEHGISVYASIPLSEWQKARDSVKTIKGIKRYKQSQLLAVGNPTDLAIE
                     AIRSLRTSLHFAMMQAQNNVLMMTGVSPSIGKTFVCANLAAVISQTNKRVLLIDCDMR
                     KGYTHELLGTNNVNGLSEILIGQGDITTAAKPTSIAKFDLIPRGQVPPNPSELLMSER
                     FAELVKWASKNYDLVLIDTPPILAVTDAAIVGRHVGTTLMVARYAVNTLKEVETSLSR
                     FEQNGIPVKGVILNSIFRRASAYQDYGYYEYEYKSDAK"
     gene            4191..5030
                     /gene="wcaA"
                     /locus_tag="NBI74_002388"
     CDS             4191..5030
                     /gene="wcaA"
                     /locus_tag="NBI74_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaA"
                     /protein_id="HCD1932712.1"
                     /translation="MKDNPLISIYMPTWNRQQLAIRAIKSVLRQDYNNWEMIIVDDCS
                     TSWEQLQQYVTALNDPRITYIHNDINSGACAVRNQAIMLAQGEYITGIDDDDEWTPNR
                     LSVFLAHKQQLVTHAFLYANDYVCQGEVYSQPASLPLYPKSPYSRRLFYKRNIIGNQV
                     FTWAWRFKECLFDTELKAAQDYDIFLRMVVEYGEPWKVEEATQILHINHGEMQITSSP
                     KKFSGYFHFYRKHKDKFDRASKKYQLFTLYQIRNKRMTWRTLLTLLSVRNGKRLADGI
                     RGR"
     gene            5033..5521
                     /gene="wcaB"
                     /locus_tag="NBI74_002389"
     CDS             5033..5521
                     /gene="wcaB"
                     /locus_tag="NBI74_002389"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaB"
                     /protein_id="HCD1932713.1"
                     /translation="MLEDLRANSWSLRPCCMVLAYRAAHFCSVWRKKNVLNNLWAAPL
                     LVLYRIITECFFGYEIQAAATIGRRFTIHHGYAVVINKNVVAGDDFTIRHGVTIGNRG
                     ADNMACPHIGNGVELGANVIILGDITLGNNVTVGAGSVVLDSVPDNALVVGEKARVKV
                     IK"
     gene            5518..6735
                     /gene="wcaC"
                     /locus_tag="NBI74_002390"
     CDS             5518..6735
                     /gene="wcaC"
                     /locus_tag="NBI74_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaC"
                     /protein_id="HCD1932714.1"
                     /translation="MNILQFNVRLAEGGAAGVALDLHQRALQHGLASHFVYGYGKGGK
                     ESVSHQNYPQVIKHTPRMTAMANIALFRLFNRDLFGNFNELYRTITRTPGPVVLHFHV
                     LHSYWLNLKSVVRFCEKVKNHKPDITLVWTLHDHWSVTGRCAFTDGCEGWKTGCQKCP
                     TLNNYPPVKIDRAHQLVAGKRQLFREMLALGCQFISPSQHVADAFNSLYGPGRCRIIN
                     NGIDMATEAILADLPPVRETQGKPKIAVVAHDLRYDGKTNQQLVREMMALGDKIELHT
                     FGKFSPFTAGNVVNHGFETDKRKLMSALNQMDALVFSSRVDNYPLILCEALSIGVPVI
                     ATHSDAAREVLQKSGGKTVSEEDVLQLVQLSKPEIAQAIFGTTLAEFSQRSRAAYSGQ
                     QMLEEYVNFYQNL"
     gene            6710..7927
                     /gene="wcaD"
                     /locus_tag="NBI74_002391"
     CDS             6710..7927
                     /gene="wcaD"
                     /locus_tag="NBI74_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative colanic acid polymerase WcaD"
                     /protein_id="HCD1932715.1"
                     /translation="MSTSIRICSYLLLPLIYLLVNVKIAQLGESFPITIVTFLPVLLL
                     LFLERISVKKLMIALGIGAGLTAFNYLFGQSLDASKYVTSTMLFVYIVIIIGMVWSIR
                     FKTISPHNHRKILRFFYLVVGLVVVLAAVEMAQIILTGGSSIMESISKYLIYSNSYVL
                     NFIKFGGKRTTALYFEPAFFALALISIWLSIKQFGIKTPKTDAMILAGIILSGSFSGV
                     MTFILFYLLEWAFQYLNKEAIKKKLPLALISLAVFLVGVVIAFPYISTRLGDLGTEGS
                     SSYYRIVGPLVMVGYSLTHIDGVVRFGSLYEYVASFGIFNGADVGKTIDNGLYLLIIY
                     FSWFAVFLSLWYMGKVIKMMINAFGDNRNFRVQLYLFTPVSLFFTGSIFSPEYAFLIV
                     CPFILRKALNITR"
     gene            7938..8684
                     /gene="wcaE"
                     /locus_tag="NBI74_002392"
     CDS             7938..8684
                     /gene="wcaE"
                     /locus_tag="NBI74_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaE"
                     /protein_id="HCD1932716.1"
                     /translation="MLLSIITVAFRNLAGIVKTHASLAHLAQADDISFEWIVVDGGSN
                     DGTREYLENLNGIYNLRFVSEPDNGIYDAMNKGIEMAQGKFALFLNSGDIFHQDAAYF
                     VRKLKVQKDNVMITGDALLDFGDGHKIKRSAKPGWYIYHSLPASHQAIFFPVSGLKKW
                     RYDLEYKVSSDYALAAKMYKAGYAFKKLNGLVSEFSMGGVSTTNNMELCADAKKVQRQ
                     ILHVPGFWAELSWHLRQRTTSKTKALYNKS"
     gene            8700..9248
                     /gene="wcaF"
                     /locus_tag="NBI74_002393"
     CDS             8700..9248
                     /gene="wcaF"
                     /locus_tag="NBI74_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaF"
                     /protein_id="HCD1932717.1"
                     /translation="MQDLSGFSVPKGFRGGNAIKVQLWWAVQATIFAWSPQVLYRWRA
                     FLLRLFGAKIGKNVVIRPSVKITYPWKLTLGDYAWVGDDVNLYTLGEITIGAHSVISQ
                     KSYLCTGSHDHASQHFTINATPIVIGEKCWLATDVFVAPGVTIGDGTVVGARSSVFKS
                     LPANVVCRGNPAVVIRKRVETE"
     gene            9275..10396
                     /gene="gmd"
                     /locus_tag="NBI74_002394"
     CDS             9275..10396
                     /gene="gmd"
                     /locus_tag="NBI74_002394"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HCD1932718.1"
                     /translation="MSKVALITGVTGQDGSYLAEFLLEKGYEVHGIKRRASSFNTERV
                     DHIYQDPHTCNPKFHLHYGDLSDTSNLTRILREVQPDEVYNLGAMSHVAVSFESPEYT
                     ADVDAMGTLRLLEAIRFLGLEKKTRFYQASTSELYGLVQEIPQKETTPFYPRSPYAVA
                     KLYAYWITVNYRESYGMYACNGILFNHESPRRGETFVTRKITRAIANIAQGLESCLYL
                     GNMDSLRDWGHAKDYVKMQWMMLQQEQPEDFVIATGVQYSVRQFVEMAAAQLGIKLRF
                     EGTGVEEKGIVVSVTGHDAPGVKPGDVIIAVDPRYFRPAEVETLLGDPTKAHEKLGWK
                     PEITLREMVSEMVANDLEAAKKHSLLKSHGYDVAIALES"
     gene            10399..11364
                     /gene="fcl"
                     /locus_tag="NBI74_002395"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
     CDS             10399..11364
                     /gene="fcl"
                     /locus_tag="NBI74_002395"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
                     /EC_number="1.1.1.271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HCD1932719.1"
                     /translation="MSKQRIFIAGHRGMVGSAIRRQLEQRGDVELVLRTRDELNLLDS
                     RAVHDFFASESIDQVYLAAAKVGGIVANNTYPADFIYQNMMIESNIIHAAHQNDVNKL
                     LFLGSSCIYPKLAKQPMAESELLQGTLEPTNEPYAIAKIAGIKLCESYNRQYGRDYRS
                     VMPTNLYGPHDNFHPSNSHVIPALLRRFHEATAQNAPDVVVWGSGTPMREFLHVDDMA
                     AASIHVMELAHEVWQENTQPMLSHINVGTGVDCTIRELAQTIAKVVGYKGRVVFDASK
                     PDGTPRKLLDVTRLHQLGWYHEISLEAGLASTYQWFLENQDRFRG"
     gene            11367..11846
                     /gene="gmm"
                     /locus_tag="NBI74_002396"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
     CDS             11367..11846
                     /gene="gmm"
                     /locus_tag="NBI74_002396"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose mannosyl hydrolase"
                     /protein_id="HCD1932720.1"
                     /translation="MFLRQEDFATVVRSTPLVSLDFIVENSRGEFLLGKRTNRPAQGY
                     WFVPGGRVQKDETLEAAFERLTMAELGLRLPITAGQFYGVWQHFYDDNFSGTEFTTHY
                     VVLGFRIRVAEDELLLPDEQHDDYRWLTPDALLASDNVHANSRAYFLSEKRAGVPGL"
     gene            11843..13057
                     /gene="wcaI"
                     /locus_tag="NBI74_002397"
     CDS             11843..13057
                     /gene="wcaI"
                     /locus_tag="NBI74_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis fucosyltransferase
                     WcaI"
                     /protein_id="HCD1932721.1"
                     /translation="MKILVYGINYSPELTGIGKYTGEMVEWLAAQGHEVRVITAPPYY
                     PQWQVGENYSAWRYKREEGAATVWRCPLYVPKQPSTLKRLLHLGSFAVSSFFPLMAQR
                     RWKPDRIIGVVPTLFCTPGMRLLAKLSGARTVLHIQDYEVDAMLGLGLAGKGKVAQLA
                     TAFERSGLHNVDNVSTISRSMMNKAIEKGVAAENVIFFPNWSEIARFQHVADADVDAL
                     RNQLGLPDNKKIILYSGNIGEKQGLENVIEAADRLRDEPLIFAIVGQGGGKARLEKMA
                     QQRGLRNMQFFPLQSYDALPALLKMGDCHLVVQKRGAADAVLPSKLTNILAVGGNAVI
                     TAEAHTELGQLCETFPGIAVCVEPESVEALVAGIRQALLLPKHNTVAREYAERTLDKE
                     NVLRQFINDIRG"
     gene            13060..14496
                     /gene="cpsB"
                     /locus_tag="NBI74_002398"
     CDS             13060..14496
                     /gene="cpsB"
                     /locus_tag="NBI74_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanyltransferase"
                     /protein_id="HCD1932722.1"
                     /translation="MAQSKLYPVVMAGGSGSRLWPLSRVLYPKQFLCLKGDLTMLQTT
                     ICRLNGVECESPVVICNEQHRFIVAEQLRQLNKLTENIILEPAGRNTAPAIALAALAA
                     KRHSPENDPLMLVLAADHVIADEDAFRAAVRNAMPYAEAGKLVTFGIVPDLPETGYGY
                     IRRGEVSAGEQDAAAFEVAQFVEKPNLETAQAYVASGEYYWNSGMFLFRAGRYLEELK
                     KYRPDILDACEKAMSDVDPDLDFIRVDEEAFLACPEESVDYAVMERTADAVVVPMDAG
                     WSDVGSWSSLWEICAHTAEGNVCHGDVINHKTENSYVYAESGLVTTVGVKDLVVVQTK
                     DAVLIADRNAVQDVKKVVEQIKADGRHEHRVHREVYRPWGKYDSIDAGDRYQVKRITV
                     KPGEGLSVQMHHHRAEHWVVVAGTAKVTIDGDIKLLGENESIYIPLGATHCLENPGKI
                     PLDLIEVRSGSYLEEDDVVRFADRYGRV"
     gene            14689..16059
                     /gene="cpsG"
                     /locus_tag="NBI74_002399"
                     /gene_synonym="manB"
     CDS             14689..16059
                     /gene="cpsG"
                     /locus_tag="NBI74_002399"
                     /gene_synonym="manB"
                     /EC_number="5.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis phosphomannomutase
                     CpsG"
                     /protein_id="HCD1932723.1"
                     /translation="MKKLTCFKAYDIRGKLGEELNEDIAWRIGRAYGEFLKPKTIVLG
                     GDVRLTSETLKLALAKGLQDAGVDVLDIGMSGTEEIYFATFHLGVDGGIEVTASHNPM
                     DYNGMKLVREGARPISGDTGLRDVQRLAEANDFPPVDETKRGRYQQINLRDAYVDHLF
                     GYINFKNLTPLKLVINSGNGAAGPVVDAIEARFKALGAPVELIKVHNTPDGNFPNGIP
                     NPLLPECRDDTRNAVIKHGADMGIAFDGDFDRCFLFDEKGQFIEGYYIVGLLAEAFLE
                     KNPGAKIIHDPRLSWNTVDVVTAAGGTPVMSKTGHAFIKERMRKEDAIYGGEMSAHHY
                     FRDFAYCDSGMIPWLLVAELVCLKGKTLGELVRDRMAAFPASGEINSKLAQPVEAINR
                     VEQHFSREALAVDRTDGISMTFADWRFNLRSSNTEPVVRLNVESRGDVPLMEARTRTL
                     LTLLNE"
     gene            16114..17508
                     /gene="wcaJ"
                     /locus_tag="NBI74_002400"
     CDS             16114..17508
                     /gene="wcaJ"
                     /locus_tag="NBI74_002400"
                     /EC_number="2.7.8.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate glucose
                     phosphotransferase"
                     /protein_id="HCD1932724.1"
                     /translation="MTNLKKRERAKTNASLISMVQRFSDITIMFAGLWLVCEVSGLSF
                     LYMHLLVALITLVVFQMLGGITDFYRSWRGVRAATEFALLLQNWTLSVIFSAGLVAFN
                     NDFDTQLKIWLAWYGLTSIGLVVCRSCIRIGAGWLRNHGYNKRMVAVAGDLAAGQMLM
                     ESFRNQPWLGFEVVGVYHDPKPGGVSNDWAGNLQQLVEDAKAGKIHNVYIAMQMCDGA
                     RVKKLVHQLADTTCSVLLIPDVFTFNILHSRLEEMNGVPVVPLYDTPLSGVNRLIKRA
                     EDIVLATLILLLISPVLCCIALAVKLSSPGPVIFRQTRYGMDGKPIKVWKFRSMKVME
                     NDKVVTQATQNDPRVTKVGNFLRRTSLDELPQFINVLTGGMSIVGPRPHAVAHNEQYR
                     QLIEGYMLRHKVKPGITGWAQINGWRGETDTLEKMEKRVEFDLEYIREWSVWFDIKIV
                     FLTVFKGFVNKAAY"
     gene            17510..18988
                     /gene="wzxC"
                     /locus_tag="NBI74_002401"
     CDS             17510..18988
                     /gene="wzxC"
                     /locus_tag="NBI74_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid undecaprenyl disphosphate flippase
                     WzxC"
                     /protein_id="HCD1932725.1"
                     /translation="MSLREKTISGAKWSAIATVIIIGLGLVQMTVLARIIDNHQFGLL
                     TVSLVIIALADTLSDFGIANSIIQRKEISHLELTTLYWLNVGLGLVVCVAVFLLSDLI
                     GDVLNNPDLAPLIKTLSLAFVVIPHGQQFRALMQKELEFNKIGMIETSAVLAGFTFTV
                     VSAHFWPLAMTAILGYLVNSAVRTLLFGYFGRKIYRPGLHFSLASVAPNLRFGAWLTA
                     DSIINYLNTNLSTLVLARILGAGVAGGYNLAYNVAVVPPMKLNPIITRVLFPAFAKIQ
                     DDTEKLRVNFYKLLSVVGIINFPALLGLMVVSNNFVPLVFGEKWNSIIPVLQLLCVVG
                     LLRSVGNPIGSLLMAKARVDISFKFNVFKTFLFIPAIVMGGQMAGAIGVTLGFLLVQI
                     INTILSYFVMIKPVLGSSYRQYILSLWLPFYLSLPTLVVSYALGIVLKGQLALGMLLA
                     VQIAAGVLAFVVMIVLSRHSLVVEVKRQFCRSEKMKMLLRAG"
     gene            19552..20832
                     /gene="wcaK"
                     /locus_tag="NBI74_002402"
     CDS             19552..20832
                     /gene="wcaK"
                     /locus_tag="NBI74_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis pyruvyl transferase
                     WcaK"
                     /protein_id="HCD1932726.1"
                     /translation="MKLLILGNHTCGNRGDSAILRGLLDAINILNPHAEVDVMSRYPV
                     SSSWLLNRPVMGDPLFLQMKQHNSAAGVVGRVKKVLRRRYQHQVLLSRVTDTGKLRNI
                     AIAQGFTDFVRLLSGYDAIIQVGGSFFVDLYGVPQFEHALCTFMAKKPLFMIGHSVGP
                     FQDEQFNQLAKYVFGRCDALILRESVSLDLMKRSNITTAKVEHGVDTAWLVDHHTEDF
                     TASYAVQHWLDVAAQQKAVAITLRELAPFDKRLGTTQQAYEKAFAGVVNRILDEGYQV
                     IALSTCTGIDSYNKDDRMVALNLRQHISDPARYHVVMDELNDLEMGKILGACELTVGT
                     RLHSAIISMNFATPAIAINYEHKSAGIMQQLGLPEMAIDIRHLLDGSLQAMVADTLGQ
                     LPALNTRLNEAVSRERQTGMQMVKSVLERIGEAK"
     gene            20829..22049
                     /gene="wcaL"
                     /locus_tag="NBI74_002403"
     CDS             20829..22049
                     /gene="wcaL"
                     /locus_tag="NBI74_002403"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaL"
                     /protein_id="HCD1932727.1"
                     /translation="MKVGFFLLKFPLSSETFVLNQITAFIDMGFEVEIVALQKGDTQN
                     THAAWTKYNLAARTRWLQDEPTGKVAKLRHRASQTLRGIHRKNTWQALNLKRYGAESR
                     NLILSAICGQVATPFHADVFIAHFGPAGVTAAKLRELGVIRGKIATIFHGIDISSREV
                     LNHYTPEYQQLFRRGDLMLPISDLWAGRLQKMGCPREKIAVSRMGVDMTRFSPRPVKA
                     PATPLEIISVARLTEKKGLHVAIEACRQLKEQGVAFRYRILGIGPWERRLRTLIEQYQ
                     LEDVVEMPGFKPSHEVKEMLDDADVFLLPSVTGADGDMEGIPVALMEAMAVGIPVVST
                     LHSGIPELVEADKSGWLVPENDARALAQRLAAFSQLDTDELAPVVKRAREKVEHDFNQ
                     QVINRELASLLQAL"
     gene            22060..23454
                     /gene="wcaM"
                     /locus_tag="NBI74_002404"
     CDS             22060..23454
                     /gene="wcaM"
                     /locus_tag="NBI74_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis protein WcaM"
                     /protein_id="HCD1932728.1"
                     /translation="MPFKKISRRTFLTASSVLAFLHTPFARALPARPSVNINDYNPHD
                     WIASFKQAFSEGQTVVVPAGLVCDNINTGIFIPPGKTLHILGSLRGNGRGRFVLQDGS
                     QVTGEEGGSMHNITLDVRGSDCTIKGLAMSGFGPVTQIYIGGKNKRVMRNLTIDNLTV
                     SHANYAILRQGFHNQIIGANITNCKFSDLQGDAIEWNVAINDRDILISDHVIERINCT
                     NGKINWGIGIGLAGSTYDNNYPENQAVKNFVVANITGSDCRQLIHVENGKHFVIRNIK
                     ARNITPDFSKKAGIDNATVAIYGCDNFVIDNIEMINSAGMLIGYGVIKGKYLSIPQNF
                     RVNDIQLDNTHLAYKLRGIQISAGNAVSFVALTNIEVKRASLELHNKPQHLFMRNIKV
                     MQESSVGPALSMNFDMRKDVRGVFMAKKETLLSLANVHAVNERGQSSVDIDRINHHIV
                     NVEKINFRLPERRE"
     gene            23629..24522
                     /gene="galF"
                     /locus_tag="NBI74_002405"
     CDS             23629..24522
                     /gene="galF"
                     /locus_tag="NBI74_002405"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalF"
                     /protein_id="HCD1932729.1"
                     /translation="MTNLKAVIPVAGLGMHMLPATKAIPKEMLPIVDKPMIQYIVDEI
                     VAAGIKEILLVTHASKNAVENHFDTSYELESLLEQRVKRQLLAEVQSICPPGVTIMNV
                     RQGEPLGLGHSILCARPAIGDNPFVVVLPDVVIDDASADPLRYNLAAMIARFNETGRS
                     QVLAKRMPGDLSEYSVIQTKEPLDREGKVSRIVEFIEKPDQPQTLDSDIMAVGRYVLS
                     ADIWPELERTQPGAWGRIQLTDAIAELAKKQSVDAMLMTGDSYDCGKKMGYMQAFVKY
                     GLRNLKEGAKFRKGIEKLLSE"
     gene            24895..25980
                     /gene="rfbB"
                     /locus_tag="NBI74_002406"
     CDS             24895..25980
                     /gene="rfbB"
                     /locus_tag="NBI74_002406"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HCD1932730.1"
                     /translation="MKILVTGGAGFIGSAVVRHIINNTQDSVVNVDKLTYAGNLESLA
                     EISDSERYSFEHADICDAEAMARIFAQHQPDAVMHLAAESHVDRSITGPAAFIETNIV
                     GTYVLLEAARNYWSGLDDEKKKNFRFHHISTDEVYGDLPHPDEVNSNETLQLFTETTA
                     YAPSSPYSASKASSDHLVRAWKRTYGLPTIVTNCSNNYGPYHFPEKLIPLVILNALEG
                     KALPIYGKGDQIRDWLYVEDHARALYTVVTEGKAGETYNIGGHNEKKNIDVVFTICDL
                     LDEIVPKEKSYREQITYVADRPGHDRRYAIDADKISRELGWKPQETFESGIRKTVEWY
                     LANTNWVENVKSGAYQSWIEQNYEGRQ"
     gene            25980..26879
                     /gene="rfbD"
                     /locus_tag="NBI74_002407"
     CDS             25980..26879
                     /gene="rfbD"
                     /locus_tag="NBI74_002407"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HCD1932731.1"
                     /translation="MNILLFGKTGQVGWELQRALAPLGNLIALDVHSTDYCGDFSNPE
                     GVAETVKKIRPDVIVNAAAHTAVDKAESEPEFAQLLNATSVEAIAKAANEVGAWVIHY
                     STDYVFPGTGEIPWQETDATAPLNVYGETKLAGEKALQKHCAKHIIFRTSWVYAGKGN
                     NFAKTMLRLAKEREELAVINDQFGAPTGAELLADCTAHAIRVAVDKPEVAGLYHLVAG
                     GTTTWHDYAALVFEEARKAGINLALNKLNSVPTTAYPTPARRPHNSRLNTEKFQQNFA
                     LVLPDWQVGVKRMLNELFTTTAI"
     gene            26937..27818
                     /gene="rfbA"
                     /locus_tag="NBI74_002408"
     CDS             26937..27818
                     /gene="rfbA"
                     /locus_tag="NBI74_002408"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HCD1932732.1"
                     /translation="MKMRKGIILAGGSGTRLYPVTMAVSKQLLPIYDKPMIYYPLSTL
                     MLAGIRDILIISTPQDTPRFQQLLGDGSQWGLNLQYKVQPSPDGLAQAFIIGEEFIGG
                     DDCALVLGDNIFYGHDLPKLMEAAVNKESGATVFAYHVNDPERYGVVEFDKNGTAISL
                     EEKPLEPKSNYAVTGLYFYDNDVVQMAKNLKPSARGELEITDINRIYLEQGRLSVAMM
                     GRGYAWLDTGTHQSLIEASNFIATIEERQGLKVSCPEEIAFRKGFIDVEQVRKLAVPL
                     IKNNYGQYLYKMTKDSN"
     gene            27818..>28298
                     /gene="rfbC"
                     /locus_tag="NBI74_002409"
     CDS             27818..>28298
                     /gene="rfbC"
                     /locus_tag="NBI74_002409"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HCD1932733.1"
                     /translation="MNVIRTEIEDVLILEPRVFGDDRGFFYESFNQSAFEHILGYPVS
                     FVQDNHSRSSKNVLRGLHFQRGEYAQDKLVRCTHGAVFDVAVDIRPNSVSFGKWVGVL
                     LSADNKQQLWIPKGFAHGFLVLSDIAEFQYKTTNYYHPESDCGICWNDERIAIDWPQT
                     "
BASE COUNT         7025 a         6870 c         7652 g         6751 t
ORIGIN      
        1 ctcctgcatt attggaagcc aatattcaat taccataatg ccaccttata ttcggaaatt
       61 aatttccgca ataacccttt ctcatcaacg actgtatgca gagagggttt gaaaaattaa
      121 gctaaatcag tgtattggta gctaaaaagc caggggcggt agcgtgtctg ggtgcctgaa
      181 agaccagtct gaattattct gtcaatagcc tgcggatgaa atcaattttt ttaggactga
      241 tgccagttaa atttttattc aattaactgc aaggtaaatg tgcatggcga cattgctatt
      301 aatagtgcac aggataatta ctctgccaaa gtgataaata aacaatgatg aaatccaaaa
      361 tgaaattgat gccattattg gtgtcagtaa ccttgataag cggttgcaca gtacttccgg
      421 gcagcaatat gtcgacgatg gggaaagacg tcatcaaaca gcaggacgct gatttcgacc
      481 tcgacaaaat ggtgaatgtt tatccgttaa ccccgcgcct gattgatcaa ttacgcccac
      541 gacccaacgt agcgcgcccc aatatgacgc tggaaagtga gatcgcgaat taccagtatc
      601 gcgtcgggcc gggggacgtt cttaatgtca ccgtctggga tcacccggaa ctcaccacgc
      661 cagccggtca gtaccgcagc tccagcgaca ccggcaactg ggtacagcct gacggcacca
      721 tgttctaccc gtatatcggc aaggttcacg tcgtggggaa aacgctcgcc gaaatccgca
      781 gtgatattac cgggcgctta gcaacgtata tcgctgaccc gcaggtggac gttaatatcg
      841 ccgccttccg ctcgcaaaaa gcctatatct ccggtcaggt gaataaatcc ggtcaacagg
      901 ccattaccaa cgtgcctctg accattctcg acgctatcaa cgccgcaggt ggcctgaccg
      961 acaccgctga ctggcgcaac gtggtgctaa cacacaatgg tcgtgaagaa cgcatttctt
     1021 tgcaggcgct gatgcaaaac ggcgacctca atcagaaccg actgctttac cccggcgata
     1081 ttctttacgt gccacgtaat gatgatctga aagtgtttgt gatgggtgaa gtgaagaaac
     1141 agagcaccct gaaaatggac tttagcggca tgaccctgac tgaagccctg ggcaatgctg
     1201 aaggcatcga catgaccacc tccaacgcca gcggcatctt tgtcattcgt ccgctgaaag
     1261 gcgagggcgg gcgtaacggc aagattgcca atatctacca gctggatatg tccgatgcca
     1321 cgtcgctggt gatggcgaca gaattccgcc tgcagcctta tgacgtggtg tatgtcacca
     1381 ccgccccggt ttcccgctgg aaccgtctga tcaatcagtt gctgccaact attagcggtg
     1441 tccgttacat gacggataca gccagcgaca ttcataactg gtaatcttca tgtttaacaa
     1501 catcttagtt gtctgtgtcg gcaatatttg ccgttccccg acggcggaac gcttactgca
     1561 acgttatcac ccggagctaa aagtggagtc cgctggactc ggcgcgctgg tcggtaaggg
     1621 cgctgatcct accgccatca gcgtcgccgc agaacatcaa ctgtctctgg aaggtcattg
     1681 cgcacggcaa atcagccgcc gtctgtgtcg caactacgac ctgattctga ccatggaaaa
     1741 gcgccatatc gaacgcttat gcgagatggc acccgagatg cgcggcaaag tgatgctgtt
     1801 tggtcactgg gataacgaat gtgaaatccc cgatccatat cgcaaaagcc gggaaacgtt
     1861 tacagcggtg tacgcattac ttgaacggtc tgcccgccag tgggcgcagg cattgaacgc
     1921 agagcaggta taagaatgac agaaaaagta aaacaacatg ccgctccggt aacgggcagt
     1981 gatgaaatcg atattggtcg cctggtcggc accgtcattg aagcgcgctg gtgggtgatt
     2041 ggcatcaccg ccgtatttgc cctctgtgcc gtggtttaca ccttctttgc tacgccgatt
     2101 tatagcgccg acgcactggt acaaatcgag caaagcagcg gcaattcgtt agtgcaggat
     2161 atcggatcgg cattagccaa caaaccgcct gcatcggacg ccgaaatcca gttgattcgt
     2221 tcgcgcctgg tgcttggtaa aacggtggac gatctcgacc tcgatattgc ggtgagcaaa
     2281 aacacgttcc cgattttcgg tgcgggctgg gagcgcctga tgggacgcca gaacgagacg
     2341 gtgaaagtga ccacctttaa ccgaccgaag gagatggcgg atcaggtgtt tacgcttaat
     2401 gtgctggaca acaaaaacta caccctgagc agcgatggcg gctttagtgc ccgtgggcaa
     2461 gcgggccaga tgctgaaaaa agaaggtgtc acgctgatgg ttgaagccat tcacgccagc
     2521 ccgggcagtg agtttaccgt caccaaatac tccacgctgg ggatgatcaa ccaactgcaa
     2581 aacagcctga cggtaacgga gaacggcaaa gacgcaggtg ttctgagcct gacttacacc
     2641 ggtgaagatc gcgaacagat ccgcgacatt cttaacagca tcgcccgtaa ctatcaggag
     2701 caaaatattg agcgcaaatc ggcggaagcg tcgaaaagcc tcgctttcct cgcgcaacag
     2761 ttaccggaag tacgtagccg ccttgatgtt gccgaaaaca aactgaatgc cttccgtcag
     2821 gataaagatt cagttgatct gccgctggaa gcgaaagcgg tgctcgattc gatggtgaac
     2881 atcgacgcgc agttgaacga actgaccttt aaagaggcgg aaatctccaa gctgtacacc
     2941 aaagttcacc cggcgtaccg cacgctgctg gagaaacggc aggcgctgga agacgaaaaa
     3001 gccaaactta acggtcgcgt aacggcgatg ccgaaaaccc agcaggaaat tgtgcgtctg
     3061 acccgcgatg tcgagtctgg tcagcaggtc tatatgcagc tgctgaataa agagcaggag
     3121 ctgaaaatta ccgaagccag caccgtcggc gatgtgcgca ttgttgaccc ggcaatcacc
     3181 cagccaggtg tactgaaacc gaagaaaggg ctgattatcc ttggggcgat tatcctcggc
     3241 ctgatgctct ctatcgtggg tgtgctgctg cgctcgttgt ttaatcgcgg cattgaaagc
     3301 ccgcaagtgc tggaagaaca cggtatcagc gtctatgcca gcatcccgct gtcggagtgg
     3361 cagaaagcgc gcgatagcgt caaaaccatc aaagggatta aacgctataa acagagccag
     3421 ctactggcgg tggggaatcc aaccgatctg gcgattgaag ccatccgtag tctgcgtacc
     3481 agtctgcact tcgcgatgat gcaggcacag aacaatgtgt tgatgatgac cggggttagc
     3541 ccgtcaatcg gtaaaacctt tgtctgcgcc aacctggcgg cggtgatcag ccagaccaat
     3601 aaacgggtgt tgttgatcga ctgcgatatg cgcaaaggct acacccacga gctgttgggc
     3661 actaataacg ttaatggcct gtcggaaatt ctgattggac agggcgatat tactaccgcg
     3721 gctaaaccga cctctattgc caaatttgac ctgatcccgc gcggtcaggt gccgccaaat
     3781 ccttcagaac tgttaatgag cgaacgcttt gccgaactgg tgaagtgggc gagtaaaaac
     3841 tacgacctgg tgttgattga tacgccgccg attctggcag tgaccgatgc ggcaattgtt
     3901 ggtcgtcatg tcggaaccac attgatggtg gcgcgttatg cggtcaacac attgaaagaa
     3961 gtggaaacca gtctgagccg ctttgagcaa aacggtattc cggtgaaagg ggtgattctg
     4021 aactccatct tccgccgcgc cagcgcgtat caggattatg gctattacga atacgaatat
     4081 aagtcggatg cgaaataacg acgcctgcat tcgcaccgcc ccgtaggccg gataaggcgt
     4141 tcacgccgca tccggcaagc aaaccagctc ataagccggg agaacaacct atgaaagaca
     4201 atccgctgat ttcaatctat atgccgacct ggaaccgcca acaactggcg attcgggcga
     4261 taaaatcggt cctgcgccag gactacaaca actgggagat gattatcgtg gatgattgct
     4321 ccacctcctg ggaacaacta caacagtacg tcaccgccct caacgatccg cgcatcactt
     4381 acattcacaa cgacattaac agcggagcgt gcgcggtacg taaccaggcg attatgctgg
     4441 cgcaagggga atacatcacc gggatcgatg acgatgatga atggacaccc aaccgcctga
     4501 gcgtcttcct cgcccataaa cagcaactgg tcacacacgc ctttttgtac gctaacgact
     4561 acgtttgcca gggcgaagtt tattcccaac cggcaagttt gccgctgtat cccaaatcgc
     4621 cgtattcacg ccgcctgttt tacaaacgca atatcattgg caatcaggtc tttacctggg
     4681 cgtggcgttt caaagagtgc ttgttcgata ccgaactgaa agcggcacag gattacgaca
     4741 tcttcctgcg gatggtggtg gagtacggcg aaccgtggaa agtagaagag gcgacgcaga
     4801 tcctgcatat caatcacggc gaaatgcaga tcacctcgtc accgaaaaaa ttctccggat
     4861 acttccactt ttaccgcaag cacaaagaca aattcgaccg tgccagcaaa aaatatcagc
     4921 tgtttaccct ctaccagatc cgcaataaac gcatgacctg gcgcactttg ctgacgctgc
     4981 tgtcggtgcg taacggcaaa cgtctggctg atggtattcg ggggcgctaa agatgctgga
     5041 agatctgcgc gccaacagct ggagtttacg cccgtgctgc atggttcttg cctatcgcgc
     5101 cgctcatttt tgttcggtgt ggcgcaaaaa gaacgtcctc aacaatctgt gggcggcccc
     5161 gctgctggtg ctgtatcgca ttatcaccga atgctttttc ggttatgaaa tccaggccgc
     5221 cgcgaccata ggccgacgct ttactatcca tcacggttac gccgtggtga tcaataaaaa
     5281 cgtggtcgcg ggggatgatt tcaccattcg tcacggcgtc actatcggca atcgtggtgc
     5341 cgataacatg gcatgtccac acattggcaa cggcgtcgaa ctcggtgcca acgtcattat
     5401 tcttggtgat atcacgcttg ggaacaacgt caccgtgggc gcgggcagcg tggtgctcga
     5461 ctcggtcccg gacaacgcgc tggtggtggg agaaaaagcg cgagtgaagg taattaaatg
     5521 aatattctgc aatttaatgt gcgcctggcg gaaggcgggg cggctggagt ggcgttagat
     5581 ctccaccagc gtgcgctgca acatgggctg gcgtcacatt ttgtctacgg ctacggcaaa
     5641 ggcggcaaag agagcgtcag ccatcagaac tatccgcagg tcatcaaaca tacgccgcgg
     5701 atgaccgcga tggcgaacat tgcgttgttt cgtctgttta atcgcgatct gttcggcaat
     5761 ttcaatgagt tatatcgcac cattactcgc acaccgggtc cggtggtcct gcattttcat
     5821 gtgctgcaca gctactggtt gaatcttaag agcgtggtgc gcttttgcga aaaagtgaaa
     5881 aaccataaac cggatatcac actggtctgg acgctgcacg atcactggag cgttaccgga
     5941 cgctgcgcct ttaccgacgg ttgcgaaggc tggaaaacgg gctgccagaa atgcccgacc
     6001 ttaaataatt atccgccggt gaagattgat cgcgcacacc aactggtggc gggcaaacgg
     6061 cagttattcc gcgagatgct ggcattgggc tgtcagttta tttcccccag ccagcatgtg
     6121 gcagacgctt tcaatagtct gtacggtcca gggcgttgcc ggattatcaa taacggtatt
     6181 gatatggcaa ccgaagcgat tctggcggac ttgcctccgg tgcgcgaaac tcagggcaag
     6241 ccgaaaatcg cagtggtggc gcatgatctg cgttacgacg gcaaaactaa ccagcaactg
     6301 gtgcgcgaga tgatggcgct gggtgacaaa atcgaactgc atactttcgg taagttctca
     6361 ccgttcaccg ctggtaatgt ggtcaatcac ggctttgaaa ccgacaaacg taaactgatg
     6421 agcgcgctca atcagatgga tgcgctggta ttcagttctc gcgtcgataa ctacccgctg
     6481 attttgtgtg aggcgttatc gattggtgtg ccggtgatcg ccacccatag cgatgcggcg
     6541 cgggaagtgc tgcaaaaatc cggcggtaaa accgtcagcg aagaagatgt gctgcaactg
     6601 gtgcagttaa gcaaaccgga aatcgcacag gcgatatttg gtaccacgct ggctgagttc
     6661 agccagcgca gccgcgccgc ctacagtgga caacagatgc tggaggagta tgtcaacttc
     6721 tatcagaatc tgtagctacc tgctgctgcc gctgatctac ctgctggtta acgtcaaaat
     6781 cgcccagctt ggcgaaagtt tccccatcac catcgtcact tttttacctg tcttgttact
     6841 gctgttttta gagcgcatca gcgttaaaaa attgatgatt gccttaggga ttggcgcggg
     6901 actcacggcg tttaattacc tgtttggtca gtcgttggat gccagtaaat acgtcacttc
     6961 aaccatgcta tttgtctata ttgtgatcat tattggcatg gtgtggagta ttcgctttaa
     7021 aacgatttcg ccacacaacc atcgcaagat attacgtttc ttttatctgg tggtcgggct
     7081 ggtggtggtg ctggcggcgg tggagatggc gcaaattatc ctcaccggcg gtagcagtat
     7141 tatggagtcg atttcgaaat atctgattta cagcaacagc tatgtgctga atttcattaa
     7201 attcggcggc aagcgcacga cagcacttta tttcgaaccg gcatttttcg ctctggcatt
     7261 aatctcaatt tggctcagca tcaaacagtt tggtatcaaa acgcctaaaa cagatgctat
     7321 gattctcgca gggataatat tatccggttc attttcaggg gttatgacct ttatcctttt
     7381 ttatttgctg gagtgggcat ttcaatatct gaataaagag gcgattaaga aaaagttacc
     7441 gttagcattg atttctctgg ctgtattcct ggttggtgtg gtaatcgcgt tcccttatat
     7501 ttccacccgt ctgggcgatt taggtacaga aggatcgtca tcgtattatc gtattgtcgg
     7561 tccgctggtg atggtcggtt attctttaac ccatattgac ggtgtagtca gatttggctc
     7621 actttatgaa tatgtcgcat cattcggaat atttaacggt gcggatgtcg gaaaaaccat
     7681 agacaatggt ttgtatctgc tgattattta tttttcctgg ttcgcagtgt ttttatcgct
     7741 gtggtacatg ggaaaagtga taaaaatgat gatcaacgct tttggtgata accgcaattt
     7801 tcgcgtgcaa ttatatcttt ttactccggt atcgctgttt tttaccggtt cgatatttag
     7861 cccggaatat gcgtttttaa tcgtctgtcc gtttattttg cgaaaagcgt taaatattac
     7921 gaggtaagaa taagaacatg ttgcttagca taatcactgt cgcgtttcgt aacctcgcag
     7981 ggatagtcaa aacacacgcc tcgctggcgc atctggcgca ggcggacgat atcagcttcg
     8041 aatggattgt tgttgatggc ggttccaacg acggcacccg tgagtatctg gaaaatctca
     8101 atggtatcta taacctacgc tttgtcagcg aaccagataa cggtatctac gacgccatga
     8161 acaaaggtat tgaaatggcg caaggcaagt tcgcgttgtt tctcaactcg ggcgatattt
     8221 ttcatcagga tgccgcatat tttgtccgta agttaaaagt gcaaaaagat aacgtgatga
     8281 tcaccggcga tgcgctgctg gattttggcg acgggcataa aattaaacgt agcgccaaac
     8341 cgggttggta tatttaccac agcctgcccg ccagtcatca ggcgatattt ttcccggtgt
     8401 ccggcttgaa gaaatggcgt tatgacctgg agtataaagt ttcttccgat tacgcgctgg
     8461 cggccaaaat gtataaagcc ggttatgcat ttaaaaaact caatggcctg gtgtctgaat
     8521 tttctatggg tggggtatct accaccaata atatggaatt gtgtgctgac gccaaaaaag
     8581 tccaacggca aatattgcac gtgcctggct tttgggctga attatcctgg catttacgcc
     8641 aacgtacgac ctcaaagacg aaagccttat ataacaaaag ctgaatataa gggaaaacga
     8701 tgcaggattt aagtggattc tcggtgccga aagggttccg gggtggcaac gctattaaag
     8761 tgcaattgtg gtgggcagta caggcaacaa tatttgcctg gtcgccacaa gtattgtatc
     8821 gctggcgggc atttttatta cgtttattcg gcgcaaaaat aggaaaaaac gtagtcattc
     8881 gtccgtcagt aaaaattacc tatccgtgga aattaacgtt aggtgattac gcgtgggtcg
     8941 gcgatgacgt caatttatat accctcggtg aaataaccat tggcgcacat tcggtgatat
     9001 cgcaaaaaag ttatttatgc actggtagcc acgaccatgc aagtcaacat ttcaccatta
     9061 acgccacgcc tattgtgatt ggcgagaaat gctggctggc aaccgatgtc tttgttgccc
     9121 ctggcgtcac gatcggcgac ggcaccgtcg tgggtgcgcg aagcagtgtt tttaaatcgc
     9181 ttccggcaaa tgtggtttgt cgggggaacc ccgcagtggt gatacgcaaa cgcgttgaaa
     9241 ctgaataaat tcaaaaaata cagaggaata agacatgtca aaagtcgctc tcatcaccgg
     9301 tgtaaccgga caagacggtt cttacctggc agagtttctg ctggaaaaag gttacgaggt
     9361 gcatggtatt aagcgtcgtg catcgtcatt caacaccgag cgcgtggatc atatttatca
     9421 ggatccgcac acctgcaacc cgaaattcca tctgcattat ggcgacctga gtgatacctc
     9481 caacctgacg cgcattttgc gtgaagtgca gccggatgaa gtgtacaacc tgggcgcaat
     9541 gagccacgtt gcggtttctt ttgagtcacc ggaatatacc gcagacgtcg atgcgatggg
     9601 tacgctgcgc ctgctggagg cgatccgctt cctcggtctg gaaaagaaaa cccgtttcta
     9661 tcaggcttcc acttctgaac tgtacggtct ggtgcaggaa attccgcaga aagagaccac
     9721 gccgttctac ccacgctctc cgtatgcggt cgccaaactg tacgcctact ggatcaccgt
     9781 taactaccgc gaatcctacg gcatgtacgc ctgtaacggt attctcttca accatgaatc
     9841 cccgcgccgt ggcgagactt tcgttacccg caaaatcacc cgcgcaatcg ccaacatcgc
     9901 ccaggggctg gagtcgtgcc tgtatctcgg caatatggat tccctgcgtg actggggcca
     9961 tgccaaagac tacgtaaaaa tgcagtggat gatgctgcaa caggaacaac cggaagattt
    10021 cgttattgcc accggcgttc agtactccgt gcgtcagttc gtggaaatgg cggcggcaca
    10081 gctgggtatc aaactgcgct ttgaaggcac gggtgttgaa gagaagggca ttgtggtttc
    10141 cgtcaccggg catgacgcgc cgggcgttaa accgggtgat gtgattatcg ccgttgaccc
    10201 gcgctacttc cgtccggctg aagttgaaac gctgctcggt gacccgacca aagcgcacga
    10261 aaaactgggc tggaaaccgg aaatcaccct cagagagatg gtgtctgaaa tggtggctaa
    10321 tgaccttgaa gcggcgaaaa aacactctct gctgaaatct cacggctacg acgtggcgat
    10381 cgcgctggag tcataagcat gagtaaacaa cgcattttta tcgctggcca tcgcgggatg
    10441 gtcggttccg ccatcaggcg gcagctcgaa cagcgcggtg atgtggagct ggtattacgc
    10501 acccgcgatg aactgaacct gctggacagc cgcgcggtgc atgatttctt tgccagcgaa
    10561 agcattgacc aggtctatct ggcggcggcg aaagtgggcg gtattgttgc caacaacacg
    10621 tatccggcag atttcatcta ccagaacatg atgattgaga gcaacatcat tcacgccgcg
    10681 catcagaacg acgtgaacaa actgctgttt ctcggatcgt cctgcatcta cccgaaactg
    10741 gcaaaacagc cgatggcaga aagcgagttg ttgcagggca cgctggagcc gaccaacgag
    10801 ccttatgcca ttgccaaaat cgccgggatc aaactgtgtg aatcttacaa ccgccagtac
    10861 ggacgggatt accgctcagt catgccaacc aacctgtacg ggccacacga caacttccac
    10921 ccgagtaatt cgcatgtgat cccagcattg ctgcgtcgct tccacgaggc gacggcacag
    10981 aatgcgccgg acgtggtggt gtggggcagc ggtacgccga tgcgcgaatt tctgcacgtt
    11041 gatgatatgg cagcggcgag cattcatgtg atggagctgg cgcacgaagt ctggcaggag
    11101 aacacccagc cgatgctgtc gcacattaac gtcggcacgg gcgttgactg cactatccgt
    11161 gagctggcgc aaaccatcgc caaagtggtg ggttacaaag gtcgggtggt ttttgatgcc
    11221 agtaaaccgg atggtacgcc gcgcaaactg ctggatgtga cgcggttgca tcagcttggc
    11281 tggtatcacg aaatctcact ggaagcgggg cttgccagca cttaccagtg gttccttgag
    11341 aatcaagacc gctttcgggg gtaatgatgt ttttacgtca ggaagacttt gccacggtag
    11401 tgcgctccac accgcttgtc tctctcgact ttattgttga gaacagtcgc ggcgagtttc
    11461 tgcttggcaa aagaaccaac cgcccggcgc agggttactg gtttgtgccg ggagggcgcg
    11521 tgcagaaaga cgaaacgctg gaagccgcat ttgagcggct gacgatggcg gaactggggc
    11581 tgcgtctgcc gataacagca ggccagtttt acggtgtctg gcagcacttt tatgacgata
    11641 acttctccgg cacggaattc accactcact atgtggtgct cggttttcgc atcagagttg
    11701 cggaagatga gctgttactg ccggatgagc agcatgacga ttaccgctgg ctgacgccgg
    11761 acgcgttgct cgccagtgac aatgttcacg ctaacagccg cgcctatttt ctctctgaga
    11821 agcgtgccgg agtacccgga ttatgaaaat actggtctac ggcattaact actcgccgga
    11881 gttaaccggc atcggcaagt acaccggcga gatggtggaa tggctggcag cacaaggtca
    11941 tgaggtgcgg gtcattaccg cgccgcctta ctacccgcag tggcaggtgg gcgagaacta
    12001 ttccgcctgg cggtacaaac gggaagaggg ggccgccacg gtgtggcgct gcccgctgta
    12061 cgtgccaaaa cagccgagca ccctgaaacg cctgttgcat ctcggcagtt ttgccgtcag
    12121 cagttttttc ccgttgatgg cgcaacgccg ctggaagccg gatcgcatta tcggcgtggt
    12181 gccaacgctg ttttgcacgc cgggaatgcg cctgctggcg aagctctctg gtgcgcgtac
    12241 cgtgctgcat attcaggatt acgaagtgga cgccatgctg gggctgggcc ttgccggaaa
    12301 aggcaaagtg gcacagctgg caacggcgtt cgaacgtagc ggactgcata acgtcgataa
    12361 cgtctccacg atttcgcgct cgatgatgaa taaagccatc gaaaaaggcg tggcggcgga
    12421 aaacgtcatc ttcttcccca attggtcgga aatcgcccgt tttcaacatg ttgcagacgc
    12481 cgatgttgac gcccttcgta accagcttgg cctgccggat aacaaaaaaa tcattcttta
    12541 ctccggcaat attggtgaaa agcagggact ggaaaacgtt attgaagctg ccgatcgcct
    12601 gcgcgatgaa ccgctgattt ttgccattgt cgggcagggc ggcggcaaag cgcggctgga
    12661 aaaaatggcg cagcagcgtg gactgcgcaa catgcaattt tttccgctgc aatcgtatga
    12721 cgctttaccc gcactgctga agatggggga ttgccatctg gtggtgcaaa aacgcggcgc
    12781 ggcagatgcc gtattgccgt cgaaactgac caatattctg gcggtaggcg gtaacgcggt
    12841 gattaccgca gaagcccaca cagaactggg gcagctttgc gaaacctttc cgggcattgc
    12901 ggtttgcgtg gaaccggaat cggtcgaggc gctggtggcg gggattcgtc aggcgctcct
    12961 gctgcccaaa cacaacacgg tggcacgcga atatgccgaa cgcacgctcg ataaagagaa
    13021 cgtgttacgt caatttataa atgatattcg gggataatta tggcgcagtc gaaactctat
    13081 ccagttgtga tggcaggtgg ctccggtagc cgcttatggc cgctttcccg cgtactttac
    13141 cccaagcagt ttttatgcct gaaaggcgat ctcaccatgc tgcaaaccac catctgccgc
    13201 ctgaacggcg tggagtgcga aagcccggtg gtgatttgca atgagcagca ccgctttatt
    13261 gtcgcggaac agctgcgtca actaaataaa cttaccgaga acattattct cgaaccggcg
    13321 gggcgtaaca ccgcacctgc cattgcgctg gcggcgctgg cggcaaaacg tcatagcccg
    13381 gagaacgacc cattaatgct ggtgctggcg gcggatcatg tgattgccga tgaagacgcg
    13441 ttccgtgccg ccgtgcgtaa tgccatgccg tatgccgaag cgggcaagct ggtgaccttc
    13501 ggcattgtgc cggatctacc tgaaaccgga tatggctata ttcgtcgcgg tgaagtgtcg
    13561 gcgggtgagc aggatgcggc ggcctttgaa gtggcgcagt ttgtcgaaaa accgaatctg
    13621 gaaaccgcgc aggcctatgt ggcaagtggc gaatattact ggaacagcgg tatgttcctg
    13681 ttccgtgccg gacgctatct cgaagaactg aaaaaatatc gcccggatat tctcgacgcc
    13741 tgcgaaaagg cgatgagcga cgtcgatccg gatctcgatt ttattcgcgt ggatgaagaa
    13801 gcgtttctcg cctgcccgga agagtcggtg gattacgcgg tcatggaacg cacggcagat
    13861 gccgttgtgg tgccaatgga tgcgggctgg agcgatgtcg gttcctggtc ttcattatgg
    13921 gagatctgcg ctcacaccgc cgagggcaac gtttgccatg gcgatgtgat taatcacaaa
    13981 actgaaaaca gctatgtgta tgccgaatct ggcctggtca ccaccgtcgg ggtgaaagat
    14041 ttggtggtag tgcagaccaa agatgcagtg ctgattgccg accgtaacgc ggtgcaggat
    14101 gtgaaaaaag tggtcgagca gatcaaagct gatggtcgcc atgagcatcg ggtgcatcgc
    14161 gaagtgtatc gtccgtgggg caaatatgac tctatcgacg cgggcgaccg ctaccaggtg
    14221 aaacgcatca ccgtgaagcc gggcgaaggg ttgtcggtac agatgcatca tcaccgcgcg
    14281 gaacactggg tggttgtcgc gggaacggca aaagtcacta tcgacggtga tatcaaactg
    14341 cttggtgaaa acgagtccat ttatattccg ctgggggcga cgcattgcct ggaaaacccg
    14401 gggaaaattc cgctcgattt aattgaagtg cgctccggct cttatctcga agaggatgat
    14461 gtggtgcgct tcgcggatcg ctacggacgg gtgtaaacgt cgcatcaggc gctgtttgtc
    14521 ggatgcggtg tgaacgcctt atccgaccta cggttcgggt ttgtaggcct gataagacgc
    14581 ggcagcgtcg catcaggcaa tgaatgcgaa accgcgatgt aaataacgac aaaaacaaaa
    14641 ttggccgttt cggtcagggc caactattgc ctgaaaaagg gtaacgatat gaaaaaatta
    14701 acctgcttta aagcctatga tattcgcggg aaattaggcg aagaactgaa tgaagatatc
    14761 gcctggcgca ttggtcgcgc ctatggcgaa tttctcaaac cgaaaaccat tgtgttaggc
    14821 ggtgatgtcc gcctcaccag cgaaacctta aaactggcgc tggcgaaagg tttacaggat
    14881 gcgggcgtcg acgtgctgga tatcggcatg tccggcaccg aagaaatcta tttcgccacg
    14941 ttccatctcg gcgtggatgg cggcattgaa gttaccgcca gccataaccc gatggattac
    15001 aacggcatga agctggtgcg cgaaggggct cgcccgatca gcggcgatac cggactgcgc
    15061 gatgtccagc gtctggcaga agccaacgac tttcctcccg tcgatgaaac caaacgcggt
    15121 cgctatcagc aaatcaatct gcgtgacgct tacgttgatc acctgttcgg ttatatcaat
    15181 ttcaaaaacc ttacgccgct caagctggtg atcaactccg ggaacggcgc agcgggtccg
    15241 gtggtggacg ccattgaagc ccgctttaaa gccctcggcg caccggtgga gttaatcaaa
    15301 gtgcataaca cgccggacgg caatttcccc aacggtattc ctaacccgtt gctgccggaa
    15361 tgtcgcgacg acacccgcaa tgcggtcatc aaacacggcg cggatatggg cattgccttt
    15421 gatggcgatt ttgaccgctg tttcctgttt gacgaaaaag ggcagtttat tgagggctac
    15481 tacattgtcg gcctgctggc agaagcgttc ctcgaaaaaa atcccggcgc gaagatcatc
    15541 cacgatccac gtctctcctg gaacaccgtt gatgtggtga ctgccgcagg cggcaccccg
    15601 gtaatgtcga aaaccggaca cgcctttatt aaagaacgta tgcgcaagga agacgccatc
    15661 tacggtggcg aaatgagcgc ccaccattac ttccgtgatt tcgcttactg cgacagcggc
    15721 atgatcccgt ggctgctggt cgccgaactg gtgtgcctga aaggaaaaac gctgggcgaa
    15781 ctggtgcgcg accggatggc ggcattcccg gcaagcggtg agatcaacag caaactggcg
    15841 caacccgttg aggcgattaa ccgcgtggaa cagcacttta gccgtgaggc gctggcggtg
    15901 gatcgcaccg atggcatcag catgaccttt gccgactggc gctttaatct gcgctcctcc
    15961 aacaccgaac cggtggtgcg gttgaatgtg gaatcacgcg gtgatgtgcc gctgatggaa
    16021 gcgcgaacgc gaactctgct gacgttgctg aacgagtaat gtcggatctt cccttaccct
    16081 actgcgggta aggggctaaa aacaggaaca acgatgacaa atctaaaaaa gcgcgagcga
    16141 gcgaaaacca atgcatcgtt aatctctatg gtgcaacgct tttcagatat caccatcatg
    16201 tttgccgggc tatggctggt ttgcgaagtc agcggactgt cattcctcta catgcacctg
    16261 ttggtggcac tgattacgct ggtggtgttc cagatgctgg gcggcatcac cgatttttat
    16321 cgctcatggc gcggtgttcg ggcagcgaca gaatttgctc tgttgctaca aaactggacg
    16381 ttaagcgtga ttttcagcgc cggactggtg gcgttcaata atgatttcga cacgcaactg
    16441 aaaatctggc tggcgtggta tgggctgacc agcatcggac tggtggtttg ccgttcatgt
    16501 attcgtattg gggcgggctg gctgcgtaat catggctata acaagcgtat ggtcgcggtg
    16561 gcgggggatt tagccgccgg acaaatgctg atggagagct tccgtaatca gccgtggtta
    16621 gggtttgaag tggtgggcgt ttaccacgac ccgaaaccgg gcggcgtttc taacgactgg
    16681 gcgggtaatc tgcaacagct ggtcgaggat gcgaaagcgg gcaagattca taacgtctat
    16741 atcgcgatgc agatgtgcga cggcgcgcga gtgaaaaaac tggtccatca attggcggac
    16801 accacctgtt cggtgctgct gatccccgat gtctttacct tcaacattct ccattcacgt
    16861 ctcgaagaga tgaacggcgt tccggtggtg ccgctttacg atacgccgct ttccggggtt
    16921 aaccgcctga taaaacgtgc ggaagacatt gtgctggcga cgcttattct gctgctgatc
    16981 tccccggtgc tgtgctgtat tgcgctggcg gtgaaactga gttcaccagg gccggttatt
    17041 ttccgccaga ctcgctacgg catggatggc aagccgatca aagtgtggaa gttccgttcc
    17101 atgaaagtga tggagaacga caaagtggtg actcaggcga cgcagaacga tccgcgcgtc
    17161 actaaagtgg ggaactttct gcgccgcacc tcgctggatg aactgccgca gtttatcaat
    17221 gtgctgaccg gtgggatgtc gattgtcggt ccacgtccgc acgcagtggc gcataacgaa
    17281 cagtatcgcc agctcattga aggctacatg ctgcgccata aggtgaaacc gggcattacc
    17341 ggctgggcgc agattaatgg ctggcgcggc gaaaccgaca cgctggagaa aatggaaaaa
    17401 cgcgtcgagt tcgaccttga gtacatccgc gaatggagcg tctggttcga tatcaaaatc
    17461 gttttcctga cggtattcaa aggcttcgtt aacaaagcgg catattgata tgagcttacg
    17521 tgaaaaaacc atcagcggcg cgaagtggtc ggcgattgct acggtgatca tcatcggcct
    17581 cgggctggtg cagatgaccg tgctggcgcg gattatcgac aaccaccagt tcggtttgct
    17641 taccgtgtcg ctggtgatca tcgcgctggc agatacgctt tctgacttcg gtatcgctaa
    17701 ctcgattatt cagcgaaaag aaatcagcca cctggaactc accacgctgt actggctgaa
    17761 cgtcgggctg gggcttgtgg tgtgcgtggc ggtgtttttg ttgagtgatc tcatcggcga
    17821 cgtgctgaac aacccggacc tggcaccgtt gattaaaaca ttgtcgctgg cgtttgtggt
    17881 gatcccccac gggcaacagt tccgcgcgtt gatgcagaag gaactggagt tcaacaaaat
    17941 cggcatgatc gaaaccagcg cggtgctggc gggcttcact tttacggtgg ttagcgccca
    18001 tttctggccg ctggcgatga ccgcgatcct cggttatctg gtcaatagtg cggtaagaac
    18061 gctgctgttt ggctactttg gccgcaaaat ttatcgcccc ggtctgcatt tctcgctggc
    18121 gtcggtggca ccgaacttac gctttggtgc ctggctgacg gcggacagca tcatcaacta
    18181 tctcaatacc aacctttcaa cgctggtgct ggcgcgtatt ctcggcgcgg gcgtggcagg
    18241 gggatacaac ctggcgtaca acgtggccgt tgtgccaccg atgaagctga acccaatcat
    18301 cacccgcgtg ttgtttccgg cattcgccaa aattcaggac gacaccgaaa agctgcgcgt
    18361 taacttctac aagctgctgt cggtagtggg gattatcaac tttccggctc tgctggggct
    18421 gatggtggtg tcgaataact ttgtaccgct ggtctttggt gagaagtgga acagcattat
    18481 tccggtgctg caattgctgt gtgtggtggg tctgctgcgc tccgtaggta acccgattgg
    18541 ttcgctgctg atggcgaaag cgcgggtcga tatcagcttt aaattcaacg tattcaaaac
    18601 atttctgttt attccggcga ttgttatggg tgggcagatg gcgggcgcaa tcggcgtcac
    18661 gctcggtttc ctgctggtgc agattatcaa caccattctg agttacttcg tgatgattaa
    18721 accggttctt ggttccagtt atcgccagta catcctgagt ttatggctgc cgttttatct
    18781 ctcgctgccg acgctggtgg tcagttatgc gctgggcatt gtgctgaaag ggcaactggc
    18841 gctggggatg ctgctggcgg tgcaaatagc cgcgggcgtg ctggcgtttg tggtgatgat
    18901 tgtgctgtcg cgccattcgc tggtggtgga agtgaagcgt cagttttgtc gcagcgaaaa
    18961 aatgaaaatg cttttacggg cggggtgagt tgttgttttc ggttccgaca acggttgccg
    19021 gatgcggcgt gaacgcctta tccgtcctgg ggggctgggc ttggatcttg taggtctgat
    19081 aagacgcgtc agcgtcgcat caggcactga atgccggatg cggcgtgaac gccttatccg
    19141 tcctacggtt ctgggcttgg atcttgtagg cctgataaga cgcgccagcg tcgcatcagg
    19201 cactgaatgc cggatgcggc gtgaacgcct tatccgtcct ggggggctgg gcttggatct
    19261 tgtaggcctg ataagacgcg ttagcgtcgc atcaggcact gaatgccgga tgcggcgtaa
    19321 aacgccttat ccgtcctacg gttctgtgct cggatcttgt aggcctgata agacgcgtca
    19381 gcgtcgcatc aggcactgaa tgccggatgc ggcgtgaacg ccttatccgg cctacggttc
    19441 tgtgctcggg acttgtaggt ctgataagac gcgccagcgt cgcatcaggc aaaaaacggc
    19501 aacgaacaca acaaacacag cctcacctgg catcaaattt atgaggaaaa aatgaaatta
    19561 cttattctgg gcaaccacac ttgcggcaat cgtggcgata gcgccatcct gcgtggctta
    19621 cttgatgcca tcaatatcct caatccacat gccgaagtgg acgtgatgag ccgctatccg
    19681 gtcagttctt cctggctgct caaccgcccg gtaatgggcg atccgctgtt cctgcaaatg
    19741 aaacaacaca acagcgcagc gggcgtggtt gggcgcgtta aaaaagtcct ccgtcgccgc
    19801 taccagcacc aggtattgct ctcacgcgtc accgacactg gcaagctgcg taatatcgcc
    19861 atcgcccagg gattcaccga cttcgtgcgc ctgctgtcag gttacgacgc cattattcag
    19921 gtcggcggat cgttttttgt cgatctctac ggtgtgccgc agtttgaaca cgcgctttgc
    19981 actttcatgg cgaaaaagcc gctgtttatg attggtcaca gcgtcggccc gttccaggac
    20041 gagcaattta accaactggc gaaatacgta ttcggtcgct gcgacgcgct gatcctgcgc
    20101 gaatcggtca gccttgatct gatgaaacgc agcaatatca ccactgcaaa agttgaacat
    20161 ggcgtcgata ccgcgtggct ggtcgatcac cacacagaag acttcaccgc cagctatgct
    20221 gttcaacact ggctggatgt tgccgcacaa cagaaagcgg tggcaattac cctgcgcgaa
    20281 ctggcaccgt tcgacaaacg tctcggcacc actcaacaag cgtatgaaaa agcctttgcc
    20341 ggggtggtca atcgcattct cgacgaaggg tatcaggtga ttgcgctctc cacctgtacg
    20401 ggcatcgaca gctataacaa agacgaccgc atggtggcgc tcaatcttcg ccagcacatc
    20461 agcgatcctg cccgttacca cgtggtgatg gatgaactca acgatctgga aatgggcaaa
    20521 atcctcggtg cctgtgaact caccgtcggt acgcgcctgc actccgccat tatctcgatg
    20581 aattttgcta ctccggcaat tgccatcaac tacgaacata aatccgccgg gattatgcaa
    20641 caactggggc taccagagat ggcaattgat atccgtcatt tattagacgg cagcctgcaa
    20701 gcgatggttg cggatacctt aggccagctt ccggcactga acacgcgact taacgaagcc
    20761 gtcagtcgcg aacgtcagac gggaatgcag atggtgaaat ctgtgcttga acgcatcggg
    20821 gaggcgaaat gaaggtcggc ttctttttac tgaaatttcc actgtcgtcg gaaaccttcg
    20881 tcctcaatca aattaccgcg tttattgata tgggctttga ggtggagatt gtcgcgctgc
    20941 aaaaaggcga cacccagaac acccacgcgg catggacgaa atacaacctt gccgccagaa
    21001 cccgctggtt acaggacgaa cctacgggca aagtggcgaa actgcgccac cgcgccagcc
    21061 agaccttacg cggcattcat cgtaaaaata cctggcaggc gctcaacctc aaacgctatg
    21121 gtgctgagtc gcggaacctg attttgtctg ccatttgtgg tcaggtcgca acaccgtttc
    21181 atgctgatgt gtttatcgca cattttggcc ctgcgggggt aaccgcagca aaactacggg
    21241 aactgggtgt cattcgcggc aaaatcgcca ctatcttcca cggtattgat atctccagtc
    21301 gggaagtgct caaccattac actcccgaat atcaacaact gtttcgccgt ggcgacctga
    21361 tgttaccgat tagcgatctg tgggccggaa ggctgcaaaa aatgggctgc ccgagggaaa
    21421 aaattgccgt atcgcgcatg ggtgtagaca tgacgcgctt tagcccgcgt ccggtgaaag
    21481 cgcctgcaac gccgctggaa atcatttccg tcgcacgctt aaccgagaaa aaaggcctgc
    21541 atgtggcgat tgaagcctgc cgtcagttga aagagcaggg cgtggcattt cgctatcgca
    21601 tcctcggcat tggcccgtgg gaacgacgcc tgcgcacgct catcgaacaa tatcaactgg
    21661 aagatgtggt agagatgccg ggctttaaac caagccacga agtgaaagag atgctcgatg
    21721 acgcggatgt cttcctgttg ccatcggtta caggtgcgga tggcgatatg gaaggtattc
    21781 cggtggcgct aatggaagcg atggcggtcg gcattccggt ggtttctact ctgcatagcg
    21841 gaataccgga actggtggag gccgataaat ccggctggct ggtgcctgag aacgatgctc
    21901 gcgcactggc gcaacgactg gcggcgttta gccaactgga caccgacgaa ttggctccgg
    21961 tcgtcaaacg tgcgcgcgaa aaagtcgaac acgattttaa ccagcaggtg attaatcgag
    22021 aactcgccag cttgctgcaa gctttataga ggagaacgta tgccatttaa aaaaatctcc
    22081 cgacgcacct tcctgacggc aagctcggtg cttgccttcc tccatacccc tttcgctcgc
    22141 gcgcttcccg cccgaccaag cgttaacatt aacgactaca acccacacga ctggattgcc
    22201 tcatttaaac aagcttttag tgaagggcaa acggtcgtcg tgcctgccgg attggtttgt
    22261 gacaatatca acaccggcat cttcattcct cctggcaaaa cgttacacat ccttggaagc
    22321 ctgcgcggca acggcagagg gcgatttgtc ttacaggacg gcagccaggt gacaggggag
    22381 gagggcggca gtatgcataa catcaccctg gatgtgcgcg gctctgactg caccatcaaa
    22441 ggtctggcga tgagcggctt tggcccggta acgcagattt atatcggcgg caaaaacaaa
    22501 cgggtcatgc gcaacctgac catcgataac ctcaccgtta gccacgctaa ctacgccatc
    22561 ttacgccagg gatttcataa ccagattatc ggtgccaata tcaccaattg taagttcagc
    22621 gacttacagg gcgacgccat tgaatggaat gtagcaatca atgatcgcga cattttgata
    22681 tccgaccatg tcatcgagcg catcaactgt actaacggca aaatcaactg gggtattggc
    22741 ataggccttg cgggaagtac ctacgataat aattatccgg aaaaccaggc agtgaaaaac
    22801 tttgtcgtgg cgaatatcac gggatcagat tgtcggcaac tgatccatgt tgaaaatggt
    22861 aaacattttg ttatccgtaa tatcaaagct cgtaatatca cgccggattt cagtaagaaa
    22921 gcaggtattg ataacgccac agttgctatt tatggttgtg acaactttgt gattgataat
    22981 attgaaatga ttaatagtgc cgggatgtta attggctatg gggtaattaa aggcaaatat
    23041 ctctcgatac cgcagaattt ccgagtgaat gatattcaac tggataatac tcatcttgct
    23101 tataaattgc gcggcatcca aatctccgcc gggaacgccg tctcctttgt ggcactgact
    23161 aacattgagg tgaagcgtgc gtcgctggag ttacacaaca aaccgcaaca tctttttatg
    23221 cgaaatatca aagtgatgca ggaatcctct gttggacccg cattgagcat gaacttcgac
    23281 atgcgcaaag acgttcgcgg cgtgtttatg gcgaaaaaag aaacactgct gtctcttgca
    23341 aatgttcatg cggtgaatga aagagggcaa agctccgtcg atatcgacag gataaatcac
    23401 catattgtta atgtggaaaa gattaacttt agattgccgg aacggagaga gtagatttgc
    23461 gaccattcct ggaaaaatgg agccatactt aggaacaatg ctactgcaat ccacaacgaa
    23521 gcggcgtaac atcacaagta attcagtaat caattcaggg taattgatgc tggcgaaaaa
    23581 aatcgaacaa gctataattc agcaaccatt ttacaggtgg atgaaataat gacgaattta
    23641 aaagcagtta ttcctgtagc gggtcttggg atgcatatgt tgcctgccac taaggcgatt
    23701 cccaaagaga tgctaccgat cgtcgacaag ccaatgattc agtacatcgt tgacgagatt
    23761 gtggctgcag ggatcaaaga aatcctcctg gtaactcacg cgtccaagaa cgcggtcgaa
    23821 aaccacttcg acacctctta tgaattagaa tctctccttg aacagcgcgt gaagcgtcaa
    23881 ctgctggcgg aagtacagtc catttgcccg ccgggcgtga caattatgaa cgtgcgtcag
    23941 ggcgaacctt taggtttggg ccattccatt ttgtgtgcgc gacctgccat tggtgacaac
    24001 ccatttgtcg tggtactgcc agacgttgtg atcgacgatg ccagcgccga cccgctacgt
    24061 tacaaccttg ctgccatgat tgcgcgcttc aacgaaacgg gccgcagcca ggtgctggca
    24121 aaacgtatgc cgggtgacct ctctgaatac tccgtcattc agaccaaaga accgctggat
    24181 cgcgaaggta aagtcagccg cattgttgaa tttatcgaaa aaccggatca gccgcagacg
    24241 ctggactcag acatcatggc cgttggtcgc tatgtgcttt ctgcagatat ttggccggaa
    24301 cttgaacgca ctcagcctgg tgcatggggg cgtattcagc tgactgatgc catcgctgaa
    24361 ctggcgaaaa aacagtccgt tgatgccatg ctgatgacag gtgacagcta cgactgcggt
    24421 aaaaaaatgg ggtatatgca ggcgtttgtg aagtatgggc tgcgcaacct gaaagaaggg
    24481 gcgaagttcc gtaaagggat tgagaagctg ttaagcgagt aacaaattcc tgactggaag
    24541 tgtcggtcag taagtaatta aaaacggcag tgaagattca tagcataagt tttgctcatt
    24601 gaatctcact gccgttgtct tttttataag tacaataaaa acagcatgtt agcttgcaat
    24661 catagttcaa atttttccag gattttcctt gtttctacag ctgtttggta agacaattag
    24721 cgtttgaatt tttcggattt ggcacgagta ggttacgctc gtcacatcgt ggaaatgtac
    24781 gcagtgctct ggtagctgtt aagccagggg cggtagcgtg cattaatgca tctattaatc
    24841 aaatctggag cagtctattt cacagcatgc tctctggcta tatggaataa aaaagtgaag
    24901 atacttgtta ctggtggcgc aggatttatt ggttctgctg ttgttcgtca cataataaat
    24961 aatacgcaag atagtgttgt taatgtcgat aaattaacat acgccggaaa cctggaatca
    25021 ctcgctgaaa tttctgattc tgaacgttat tcatttgagc atgcagatat ctgcgatgcc
    25081 gaagcgatgg ctcgtatttt cgcacagcac cagccagacg cggtgatgca cctggcagca
    25141 gagagccacg ttgaccgctc aataactggc cctgcggcat ttattgaaac caatattgtg
    25201 ggtacttatg ttcttttaga agcggcgcgc aattattggt ctggtctgga tgatgaaaag
    25261 aaaaaaaact tccgttttca tcatatttct actgatgagg tgtatggtga cttaccccat
    25321 ccggatgaag taaatagcaa tgaaacgttg cagctattta cggaaacgac agcatacgcg
    25381 ccaagtagtc catattctgc ttctaaagct tccagcgatc atttggttcg cgcatggaaa
    25441 cgtacttatg gtttaccgac cattgtgact aattgctcga acaactatgg tccttatcat
    25501 ttcccggaaa agcttattcc actggttatt cttaatgcac tggaaggtaa ggcattacct
    25561 atttatggca aaggagatca gatccgcgac tggttgtatg tagaggatca tgctcgagcg
    25621 ttatataccg tcgtaaccga aggtaaagcg ggcgaaactt ataacattgg tggacacaac
    25681 gaaaagaaaa acatcgacgt agtgttcact atttgtgatt tgttggatga gatagtcccg
    25741 aaagagaaat cttatcgtga gcaaattacc tatgttgctg atcgcccagg gcatgatcgc
    25801 cgttatgcaa ttgatgccga taaaattagc cgcgaattgg gctggaaacc gcaagaaacg
    25861 tttgagagcg gtattcgtaa aacggtggaa tggtatctgg ctaatacaaa ttgggttgag
    25921 aatgtgaaaa gcggtgctta tcagtcgtgg attgaacaga actatgaggg ccgccagtaa
    25981 tgaatatcct cctattcggc aaaacagggc aggtgggttg ggaactgcag cgtgctctgg
    26041 caccgctggg taatctgatt gctcttgatg ttcactccac tgattattgt ggtgatttta
    26101 gtaatcccga aggtgtagct gaaactgtca aaaaaattcg ccctgatgtt attgttaatg
    26161 cggctgctca cactgcagta gacaaagcgg aatcagaacc ggaatttgca caattactta
    26221 acgcgacaag tgtcgaagcg attgcaaaag cagccaatga agtcggtgct tgggttattc
    26281 actactctac tgactacgta tttccgggaa ccggtgaaat accgtggcag gagacggatg
    26341 ctacagcacc tttgaatgtt tatggtgaaa ctaaactagc cggagaaaaa gcgttacaga
    26401 aacattgcgc gaagcatatt attttccgta ccagctgggt atatgcaggt aaaggaaata
    26461 acttcgccaa aacaatgttg cgtctggcaa aagagcgcga agaactggct gtgataaacg
    26521 atcagtttgg cgcaccaaca ggtgccgaat tgctggctga ttgcaccgct catgccattc
    26581 gcgtagcagt agacaaacca gaagttgctg gtttgtacca tctggttgct ggcggtacaa
    26641 caacctggca cgattatgcc gctctggtat ttgaagaagc gcgcaaagca gggattaacc
    26701 ttgctcttaa caaacttaac tccgtgccaa caacggccta tcccacacca gcccgtcgac
    26761 cccataactc tcgcctcaat acagaaaagt ttcagcagaa ctttgcgctt gtcttgcctg
    26821 actggcaggt gggtgtgaaa cgaatgctca acgaattatt tacgactaca gcaatttaat
    26881 agtttttgca tcttgttcgt gatggtggag caagatgaat taaaaggaat gatgaaatga
    26941 aaatgcgtaa aggtattatt ttagcgggtg gttctggtac acgtctttat cctgtgacta
    27001 tggctgtcag taaacagcta ttacctattt atgataaacc gatgatctat tacccgctct
    27061 ctacactgat gttggcgggt attcgcgata ttttgattat cagtacacct caggatactc
    27121 ctcgttttca acaattgctg ggtgacggta gccagtgggg cctgaatctt cagtacaaag
    27181 tgcaacctag cccagatggc ctcgcgcagg catttatcat cggtgaagag tttattggtg
    27241 gtgatgattg tgctttggtt cttggtgata atatctttta cggtcacgat ctgccgaagc
    27301 taatggaggc cgctgttaac aaagaaagtg gtgcaacggt atttgcctat cacgttaatg
    27361 atccagaacg ctatggtgtc gttgagtttg ataaaaacgg tacggcaatc agtctggaag
    27421 aaaaaccgtt agaaccaaag agtaattacg ccgttacagg tctgtacttc tatgataacg
    27481 acgtggttca gatggcgaaa aacttgaagc cgtctgcacg tggtgagtta gaaattacag
    27541 atattaaccg tatttatctt gagcagggac gtctgtctgt cgcgatgatg gggcgtggct
    27601 acgcgtggct ggacacgggg actcatcaga gtctgataga agcaagtaat tttattgcga
    27661 caattgaaga gcgccaggga ttgaaggttt cctgtcctga agagattgca tttcgtaaag
    27721 gttttattga tgttgagcaa gtaagaaaat tagctgtacc actaataaag aataattatg
    27781 ggcagtatct ttataaaatg acgaaggatt caaattaatg aatgtgatta gaactgaaat
    27841 tgaagatgtg ctaattctgg agccaagagt atttggtgat gatagaggtt tcttttatga
    27901 gagctttaat caatcagcat ttgaacatat tctaggctat ccggtcagct ttgttcaaga
    27961 caatcactca cgttcatcaa aaaatgtact cagaggcctt cactttcaac gcggcgagta
    28021 cgcacaagat aaacttgtac gctgcactca tggagcagtt tttgatgttg ctgttgatat
    28081 tcgacccaat tcggtatcct ttggtaaatg ggttggtgtt ctgctttcag ctgataataa
    28141 gcagcagttg tggataccaa aagggtttgc tcatggcttt ttggttctgt ctgatatcgc
    28201 tgaatttcaa tataaaacta caaactatta tcatcctgaa agcgattgtg gaatatgttg
    28261 gaatgatgaa cgcattgcaa ttgattggcc ccaaacat
//
LOCUS       DAHHTG010000058        27563 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.058, whole genome shotgun sequence.
ACCESSION   DAHHTG010000058 DAHHTG010000000
VERSION     DAHHTG010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 27563)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27563)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27563
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.058"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            72..1415
                     /locus_tag="NBI74_002410"
     CDS             72..1415
                     /locus_tag="NBI74_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCD1932734.1"
                     /translation="MANSITADEIREQFSQAMSAMYQQEVPQYGTLLELVADVNLAVL
                     ENNPQLHEKMVNADELARLNVERHGAIRVGTAQELATLRRMFAIMGMYPVSYYDLSQA
                     GVPVHSTAFRPIDAASLARNPFRVFTSLLRLELIENELLRQKAAEILRQRDIFTPRCR
                     QLLQEYEQRGGFNETQAQEFVQEALETFRWHQSATVDEKTYRALHNEHRLIADVVCFP
                     GCHINHLTPRTLDIDRVQSMMPECGIEPKILIEGPPRREVPILLRQTSFKALEETVLF
                     AGQKQGTHTARFGEIEQRGVALTPKGRQLYDDLLRNAGTGQDNLTHQMHLQETFRTFP
                     DSEFLMRQQGLGWFRYRLTPSGEAHRQAIHPGDDPQPLIERGWVVAQPITYEDFLPVS
                     AAGIFQSNLGNETQACNHGDASREAFEQALGCPVLDEFQLYQEAEERSKRRCGLL"
     gene            1640..3295
                     /gene="opgD"
                     /locus_tag="NBI74_002411"
                     /gene_synonym="mdoD"
     CDS             1640..3295
                     /gene="opgD"
                     /locus_tag="NBI74_002411"
                     /gene_synonym="mdoD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucan biosynthesis protein OpgD"
                     /protein_id="HCD1932735.1"
                     /translation="MDRRRFIKGSMAMAAVCGTSGIASLFSQAAFAADSDIADGQTQR
                     FDFSILQSMAHDLAQTAWRGAPRPLPDTLATMTPQAYNSIQYDAEKSLWHNVENRQLD
                     AQFFHMGMGFRRRVRMFSVDPATHLAREIHFRPELFKYNDAGVDTKQLEGQSDLGFAG
                     FRVFKAPELARRDVVSFLGASYFRAVDDTYQYGLSARGLAIDTYTDSKEEFPDFTAFW
                     FDTVKPGATTFTVYALLDSASITGAYKFTIHCEKNQVIMDVENHLYARKDIKQLGIAP
                     MTSMFSCGTNERRMCDTIHPQIHDSDRLSMWRGNGEWICRPLNNPQKLQFNAYTDNNP
                     KGFGLLQLDRDFSHYQDIMGWYNKRPSLWVEPRNKWGKGTIGLMEIPTTGETLDNIVC
                     FWQPEKAVKAGDEFAFQYRLYWSAQPPVHCPLARVMATRTGMGGFPEGWAPGEHYPEK
                     WARRFAVDFVGGDLKAAAPKGIEPVITLSSGEAKQIEILYIEPIDGYRIQFDWYPTSD
                     STDPVDMRMYLRCQGDAISETWLYQYFPPAPDKRQYVDDRVMS"
     gene            3435..3659
                     /locus_tag="NBI74_002412"
     CDS             3435..3659
                     /locus_tag="NBI74_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415943.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HCD1932736.1"
                     /translation="MFPEYRDLISRLKNENPRFMSLFDKHNKLDHEIARKEGSDGRGY
                     NAEVVRMKKQKLQLKDEMLKILQQVSVKEV"
     gene            3722..4261
                     /gene="rimL"
                     /locus_tag="NBI74_002413"
     CDS             3722..4261
                     /gene="rimL"
                     /locus_tag="NBI74_002413"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12-serine
                     acetyltransferase"
                     /protein_id="HCD1932737.1"
                     /translation="MTETIKVSESLELHAVAESHVTPLYQLICKNKTWLQQSLNWPQF
                     VQSEEDTRKTVQGNVMLHQRGYAKMFMIFKEDELIGVISFNRIEPLNKTAEIGYWLDE
                     PHQGQGIISQALQALIHHYAQSGELRRFVIKCRVDNPQSNQVALRNGFILEGCLKQAE
                     FLNDAYDDVNLYARIIDSQ"
     gene            complement(4253..5233)
                     /locus_tag="NBI74_002414"
     CDS             complement(4253..5233)
                     /locus_tag="NBI74_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LbetaH domain-containing protein"
                     /protein_id="HCD1932738.1"
                     /translation="MRKYRLSEEQRAFSYQEDGTKKSVLLRQIIAMSDFNDVIAGSAG
                     GWIDRETVLAQEGNCWIYDQNAIAFGGTVISGNTRITGTSVLWGEVYATDNVWIDNSE
                     ISQGAYISDSVTISDSLVCGQCRIFGHALIDQHSMIVAAQGLTPDHQLLLQIYDRAKV
                     SASRIVHQAQIYGDAVVRYAFIEHRAEVFDFASIEGNEENNVWLCDCAKVYGHAQVKA
                     GIEEDAIPTIHYSSQVAEYAIVEGNCVLKHHVLIGGNAVVRGGPILLDEHVVIQGESR
                     ITGAVIIENHVELTDHAVVEAFDGDTIHVRGPKVINGEERITRTLLAGLL"
     gene            5357..6349
                     /gene="tehA"
                     /locus_tag="NBI74_002415"
     CDS             5357..6349
                     /gene="tehA"
                     /locus_tag="NBI74_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate transporter/tellurite-resistance
                     protein TehA"
                     /protein_id="HCD1932739.1"
                     /translation="MQSDKVLNLPAGYFGIVLGTIGMGFAWRYASQVWQVSHWLGDGL
                     VILAMIIWGLLTGAFITRLIRFPHSVLAEVRHPVLSSFVSLFPATTMLVAIGFVPWFR
                     PLAVCLFSFGVVVQLAYAAWQTAGLWRGSHPEEATTPGLYLPTVANNFISAMACGALG
                     YTDAGLVFLGAGVFSWLSLEPVILQRLRSSGELPTALRTSLGIQLAPALVACSAWLSV
                     NGGEGDTLAKMLFGYGLLQLLFMLRLMPWYLSQPFNASFWSFSFGVSALATTGLHLGS
                     GSDNGFFHTLAVPLFIFTNFIIAILLIRTFALLMQGKLLVRTERAVLMKAEDKE"
     gene            6346..6939
                     /gene="tehB"
                     /locus_tag="NBI74_002416"
     CDS             6346..6939
                     /gene="tehB"
                     /locus_tag="NBI74_002416"
                     /EC_number="2.1.1.265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance methyltransferase TehB"
                     /protein_id="HCD1932740.1"
                     /translation="MIIRDENYFTDKYELTRTHSEVLEAVKVVKPGKTLDLGCGNGRN
                     SLYLAANGYDVDAWDKNAMSIANVERIKSIENLDNLHTRVVDLNNLTFDGQYDFILST
                     VVLMFLEAKTIPGLIANMQRCAKPGGYNLIVAAMDTADYPCTVGFPFAFKEGELRRYY
                     EGWEMVKYNEDVGELHRTDANGNRIKLRFATMLARKK"
     gene            7242..7910
                     /locus_tag="NBI74_002417"
     CDS             7242..7910
                     /locus_tag="NBI74_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3313 domain-containing protein"
                     /protein_id="HCD1932741.1"
                     /translation="MRTTSFAKVAALCGLLALSGCASKITQPDKYSGFLNNYSDLKET
                     TSATGKPVLRWVDPSFDQSKYDSIVWNPITYYPVPKPSTQVGQKVLDKILNYTNTEMK
                     EAIAQRKPLVTTAGPRSLIFRGAITGVDTSKEGLQFYEVVPVALVVAGTQMATGHRTM
                     DTRLYFEGELIDAATNKPVIKVVRQGEGKDLNNESTPMAFENIKQVIDDMATDATMFD
                     VNKK"
     gene            complement(7945..9156)
                     /gene="ydcO"
                     /locus_tag="NBI74_002418"
     CDS             complement(7945..9156)
                     /gene="ydcO"
                     /locus_tag="NBI74_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001748359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BenE family transporter YdcO"
                     /protein_id="HCD1932742.1"
                     /translation="MLRYLYVITHEVTMRLFSIPPPTLLAGFLAVLIGYASSAAIIWQ
                     AAIVAGATTAQISGWMTALGLAMGVSTLTLTLWYRVPVLTAWSTPGAALLVTGLQELT
                     LNEAIGVFIVTNALIVLCGITGLFARLMRIIPHSLAAAMLAGILLRFGLQAFASLDGQ
                     FTLCGSMLLAWLAAKAVAPRYAVIAAMIIGIVIVTAQGDVVTTDIVFKPVLPTYITPD
                     FSFAHSLSVALPLFLVTMASQNAPGIAAMKAAGYSAPVSPLIVFTGLLALFFSPFGVY
                     SVGIAAITAAICQSPEAHPDKDQRWLAAAVAGIFYLIAGLFGSAITGMMAALPVSWIQ
                     MLAGLALLSTIGGSLYQALHNERERDAAVVAFLVTASGLTLFGIGSAFWGLIAGGVCY
                     VVLNLIANRNR"
     gene            9209..9745
                     /gene="sutR"
                     /locus_tag="NBI74_002419"
     CDS             9209..9745
                     /gene="sutR"
                     /locus_tag="NBI74_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SutR"
                     /protein_id="HCD1932743.1"
                     /translation="MENLARFLSTTLKQLRQQRGWSLSRLAEATGVSKAMLGQIERNE
                     SSPTVATLWKIATGLNVPFSTFISPPQSATPSVYDPQRQAMVITSLFPYDPQLCFEHF
                     SIQMAPGAISESMPHEKGVIEHVVVIDGQLDLCVDGEWQSLNCGEGVRFAADVTHVYR
                     NGGEQTVHFHSLIHYPRS"
     gene            9818..11779
                     /gene="rlhA"
                     /locus_tag="NBI74_002420"
     CDS             9818..11779
                     /gene="rlhA"
                     /locus_tag="NBI74_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA 5-hydroxycytidine C2501 synthase"
                     /protein_id="HCD1932744.1"
                     /translation="MTVSSHRLELLSPARDAAIAREAILHGADAVYIGGPGFGARHNA
                     SNSLKDIAELVPFAHRYGAKIFVTLNTILHDDELEPAQRLITDLYQTGVDALIVQDMG
                     ILELDIPPIELHASTQCDIRTVEKAKFLSDVGFTQIVLARELNLEQIRAIHQATNATI
                     EFFIHGALCVAYSGQCYISHAQTGRSANRGDCSQACRLPYTLKDDQGRVVSYEKHLLS
                     MKDNDQTANLGALIDAGVRSFKIEGRYKDMSYVKNITAHYRQMLDAIIEERGDLARAS
                     SGRTEHFFVPSTEKTFHRGSTDYFVNARKGDIGAFDSPKFIGLPVGEVLKVAKDHIDV
                     AVTEPLANGDGLNVMIKREVVGFRANTVEKTGENQYRVWPNEMPADLHKIRPHHPLNR
                     NLDHNWHQALTKTSSERRVAVDIELGGWQEQLILTLTSEEGVSVTHTLDGQFDEANNA
                     EKAMNNLKDGLTKLGQTIYYARNVQINLPGALFVPNSLLNQFRREAADMLDAARLASY
                     QRGSRKPVADPAPVYPQTHLSFLANVYNQKAREFYHRYGVQLIDAAYEAHEEKGEVPV
                     MITKHCLRFAFNLCPKQAKGNIKSWKATPMQLVNGDEVLTLKFDCRPCEMHVIGKIKN
                     HILKMPLPGSVVASVSPDDLLKTLPKRKG"
     gene            complement(11871..12101)
                     /locus_tag="NBI74_002421"
     CDS             complement(11871..12101)
                     /locus_tag="NBI74_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2554 family protein"
                     /protein_id="HCD1932745.1"
                     /translation="MFSKALSVVLLTCALFSGQLMAGHKGHEFMWVKNVDHQLRHEAD
                     SDELRAVAEESAEGLREHFYWQKSRKPEAGQR"
     gene            12280..12516
                     /gene="hicB"
                     /locus_tag="NBI74_002422"
                     /pseudo
     CDS             12280..12516
                     /gene="hicB"
                     /locus_tag="NBI74_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415955.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HicB"
     gene            12595..14001
                     /locus_tag="NBI74_002423"
     CDS             12595..14001
                     /locus_tag="NBI74_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HCD1932746.1"
                     /translation="MKKYQQLAEQLREQIASGIWQPGDRLPSLHDQVALSGMSFMTVS
                     HAYQLLESQGYIIARPQSGYYVAPQAIKMPKATVIPVTRDEAVDINTYIFDMLQASRD
                     PSVVPFASAFPDPRLFPLQQLNRSLAQVSKTATAMSVIENLPPGNAELRQAIARRYAL
                     QGITISPDEIVITAGALEALNLSLQAVTEPGDWVIVENPCFYGALQALERLRLKALSV
                     ATDIKEGIDLQALELALQEYPVKACWLMTNSQNPLGFTLTPQKKVRLVALLNQYNVTL
                     IEDDVYSELYFGREKPLPAKAWDRHDGVLHCSSFSKCLVPGFRIGWVAAGKHARKIQR
                     LQLMSTLSTSSPMQLALVDYLSTRRYDAHLRRLRRQLAERKQRAWQALLRYLPAEVKI
                     HHNDSGYFLWLELPEPLDAGELSLVALTHHISIAPGNMFSTGENWSRFFRFNTAWQWG
                     EREEQAVKQLGKLIQERL"
     gene            14246..15391
                     /locus_tag="NBI74_002424"
     CDS             14246..15391
                     /locus_tag="NBI74_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCD1932747.1"
                     /translation="MSKTFARSSLCALSMTIMTAHAAEPPTNLENPEGRLDIIAWPGY
                     IERGQTDKQYDWVTQFEKETGCAVNVKTAATSDEMVSLMAKGGYDLVTASGDASLRLI
                     MGKRVQPINTALIPNWKTLDPRVVKGDWFNVSGKVYGTPYQWGPNLLMYNTKTFPTPP
                     DSWQVVFVEQNLPDGKSNKGRVQAYDGPIYIADAALFVKATQPQLGISDPYQLTEEQY
                     QAVLKVLRDQHSLIHRYWHDTTVQMSDFKNEGVVASSAWPYQANALKAEGQPVATVFP
                     KEGVTGWADTTMLHSEAKHPVCAYKWMNWSLTPKVQGDVAAWFGSLPVVPQGCKASPL
                     LGEKGCETNGFNYFDKIAFWKTPVAEGGKFVPYSRWTQDYIAIMGGR"
     gene            15409..16422
                     /locus_tag="NBI74_002425"
     CDS             15409..16422
                     /locus_tag="NBI74_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCD1932748.1"
                     /translation="MTYAVEFDNVSRLYGDVRAVDGVSIAIKDGEFFSMLGPSGSGKT
                     TCLRLIAGFEQLSGGAISIFGTPASNLPPWERDVNTVFQDYALFPHMSILDNVAYGLM
                     VKGVNKKQRHAMAQEALEKVALGFVQQRKPSQLSGGQRQRVAIARALVNEPRVLLLDE
                     PLGALDLKLREQMQLELKKLQQSLGITFIFVTHDQGEALSMSDRVAVFNNGRIEQVDS
                     PRDLYMRPRTPFVAGFVGTSNVFDPLMAEKLCGMTGSFALRPEHIRLNTHGEMQANGT
                     IQAVQYQGAATRFELKLNGGEKLLVSQANMTGEELPATLTPGQQVMVSWSRDVMVALV
                     EER"
     gene            16423..17364
                     /locus_tag="NBI74_002426"
     CDS             16423..17364
                     /locus_tag="NBI74_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCD1932749.1"
                     /translation="MAMNVLQSPSRPGLGKVSGFFWRNPGLGLFLLLLGPLMWFGIVY
                     FGSLLTLLWQGFYTFDDFTMSVTPELTLANIRALFNPANYDIIVRTLTMAVAVTIASA
                     ILAFPMAWYMARYTSGKMKAFFYIAVMLPMWASYIVKAYAWTLLLAKDGVAQWFLQHL
                     GLEPLLTAFLTLPAVGGNTLSTSGLGRFLVFLYIWLPFMILPVQAALERLPPSLLQAS
                     ADLGARPRQTFRYVVLPLAIPGIAAGSIFTFSLTLGDFIVPQLVGPPGYFIGNMVYSQ
                     QGAIGNMPMAAAFTLVPIVLIALYLAFVKRLGAFDAL"
     gene            17354..18148
                     /locus_tag="NBI74_002427"
     CDS             17354..18148
                     /locus_tag="NBI74_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCD1932750.1"
                     /translation="MHSERAPFFLKLAAWGGVVFLHFPILIIAAYAFNTEDAAFSFPP
                     QGLTLRWFSVAAQRSDILDAVTLSLKVAALATLIALVLGTLAAAALWRRDFFGKNAIS
                     LLLLLPIALPGIVTGLALLTAFKTINLEPGFFTIVVGHATFCVVVVFNNVIARFRRTS
                     WSLVEASMDLGANGWQTFRYVVLPNLSSALLAGGMLAFALSFDEIIVTTFTAGHERTL
                     PLWLLNQLGRPRDVPVTNVVALLVMLVTTLPILGAWWLTREGDNGQ"
     gene            18170..19594
                     /gene="patD"
                     /locus_tag="NBI74_002428"
     CDS             18170..19594
                     /gene="patD"
                     /locus_tag="NBI74_002428"
                     /EC_number="1.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminobutyraldehyde dehydrogenase"
                     /protein_id="HCD1932751.1"
                     /translation="MQHKLLINGELVSGEGEKQPVYNPATGDVLLEIAEASAEQVNAA
                     VRAADAAFAEWGQTTPKARAECLLKLADVIEENGQVFAELESRNCGKPLHSAFNDEIP
                     AIVDVFRFFAGAARCLNGLAAGEYLEGHTSMIRRDPLGVVASIAPWNYPLMMAAWKLA
                     PALAAGNCVVLKPSEITPLTALKLAELAKDIFPAGVINVLFGRGKTVGDPLTGHPKVR
                     MVSLTGSIATGEHIISHTAPSIKRTHMELGGKAPVIVFDDADIEAVVEGVRTFGYYNA
                     GQDCTAACRIYAQKGIYDTLVEKLGAAVATLKSGSPDDESTELGPLSSLAHLERVSKA
                     VEEAKATGHIKVITGGEKRKGNGYYYAPTLLAGALQDDAIVQKEVFGPVVSVTLFDSE
                     EQVVNWANDSQYGLASSVWTKDVGRAHRVSARLQYGCTWVNTHFMLVSEMPHGGQKLS
                     GYGKDMSLYGLEDYTVVRHVMVKH"
     gene            complement(19691..19786)
                     /gene="yncL"
                     /locus_tag="NBI74_002429"
     CDS             complement(19691..19786)
                     /gene="yncL"
                     /locus_tag="NBI74_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response membrane protein YncL"
                     /protein_id="HCD1932752.1"
                     /translation="MNVSSRTVVLINVFAAVGLFTLISMRFGWFI"
     gene            19982..20155
                     /gene="ortT"
                     /locus_tag="NBI74_002430"
     CDS             19982..20155
                     /gene="ortT"
                     /locus_tag="NBI74_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orphan toxin OrtT"
                     /protein_id="HCD1932753.1"
                     /translation="MSLYQHMLVFYAVMAAIAFLITWFLSHDKKRIRLLSAFLVGATW
                     PMSFPVALLFSLF"
     gene            20241..20474
                     /locus_tag="NBI74_002431"
     CDS             20241..20474
                     /locus_tag="NBI74_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcY family protein"
                     /protein_id="HCD1932754.1"
                     /translation="MSHLDEVIARVDAAIEESVIAHMNELLIALSDDAELSREDRYTQ
                     QQRLRTAIAHHGRKHKEDMEARHEQLTKGGTIL"
     gene            complement(20475..20924)
                     /locus_tag="NBI74_002432"
     CDS             complement(20475..20924)
                     /locus_tag="NBI74_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HCD1932755.1"
                     /translation="MNQSLTLAFLIAAGIGLVVQNTLMVRITQTSSTILIAMLLNSLV
                     GIVLFVSILWFKQGMAGFGELVSSVRWWTLIPGLLGSFFVFASISGYQNVGAATTIAV
                     LVASQLIGGLVLDIFRSHGVPLRALIGPICGAILLVVGAWLVARRSF"
     gene            complement(20921..21439)
                     /gene="mddA"
                     /locus_tag="NBI74_002433"
     CDS             complement(20921..21439)
                     /gene="mddA"
                     /locus_tag="NBI74_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine sulfoximine/L-methionine sulfone
                     acetyltransferase"
                     /protein_id="HCD1932756.1"
                     /translation="MSIRFARKADCAAIAEIYNHAVLYTAAIWNDQTVDADNRIAWFE
                     ARTIAGYPVLVSEEDGVVTGYASFGDWRSFDGFRHTVEHSVYVHPDHQGKGLGRKLLS
                     RLIDEARDCGKHVMVAGIESQNQASLHLHQSLGFVVTAQMPQVGTKFGRWLDLTFMQL
                     QLDERTEPDAIG"
     gene            21688..21843
                     /locus_tag="NBI74_002434"
                     /pseudo
     CDS             21688..21843
                     /locus_tag="NBI74_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310081.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
     gene            complement(21920..24022)
                     /locus_tag="NBI74_002435"
     CDS             complement(21920..24022)
                     /locus_tag="NBI74_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HCD1932757.1"
                     /translation="MKIFSVRQTVLPALLVLSPVVFAADEQTMIVSAAPQVVSELDTP
                     AAVSVVDGEEMRLATPRINLSESLTGVPGLQVQNRQNYAQDLQLSIRGFGSRSTYGIR
                     GIRLYVDGIPATMPDGQGQTSNIDLSSVQNVEVLRGPFSALYGNASGDVMNVTTQTGQ
                     QPPTIEASSYYGSFGSWRYGLKATGATGDGTQPGDVDYTVATTRFTTHGYRDHSGAQK
                     NLANAKLGVRIDDASKLSLIFNSVDIKADDPGGLTEAEWKANPQQAPRAEQYDMRKTI
                     KQTQAGLRYERSLSAQDDMSVMMYAGERETTQYQSIPMAPQLNPSHAGGVITLQRHYQ
                     GIDSRWTHRGELGVPVTFTTGLNYENMSENRKGYNNFRLNRGVPEYGQKGELRRDERN
                     LMWNVDPYLQTQWQLTDKLSLDAGVRYSSVWFDSNDHYVTPGNGDDSGDASYHKWLPA
                     GSLKYAMTDAWNVYLAAGRGFETPTINELSYRADGQSGMNFGLKPSTNDTIEIGSKTR
                     IGDGLLSLALFQTDTDDEIVVDSSSGGRTTYKNAGKTRRQGAELAWDQRFAGDFRVKA
                     SWTWLDATYRSNVCNEQDCNGNRMPGIARNMGFASIGYIPEDGWYAGTEARYMGDIMA
                     DDENTAKAPSYTLVGLFTGYKYNYHNLTVDLFGRVDNLFDKEYVGSVIVNESNGRYYE
                     PAPGRNYGVGINIAWRFE"
     gene            24264..25325
                     /locus_tag="NBI74_002436"
     CDS             24264..25325
                     /locus_tag="NBI74_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HCD1932758.1"
                     /translation="MHLRHLFSSRLRGSLLLGSLLVASSFSTQAAEEMLRKAVGKGAY
                     EMAYSQQENALWIATSQSRKLDKGGVVYRLDPVTLEVTQAIHNDLKPFGATINNTTQT
                     LWFGNTVNSAVTAIDAKTGEVKGRLVLDDRKRTEEVRPLQPRELVADDTTNTVYISGI
                     GKESVIWVVDGENIKLKTAIQNTGKMSTGLALDSKGKRLYTTNADGELITIDTTDNKI
                     LSRKKLLDDGKEHFFINISLDTTNQRAFITDSKAAEVLVVDTRNGNILAKVAAPESLA
                     VLFNPARNEAYVTHRQAGKVSVIDAKSYKVVKTFDTPTHPNSLALSADGKTLYVSVKQ
                     KSTKQQEATQPDDVIRIAL"
     gene            complement(25438..26937)
                     /gene="ansP"
                     /locus_tag="NBI74_002437"
     CDS             complement(25438..26937)
                     /gene="ansP"
                     /locus_tag="NBI74_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparagine permease"
                     /protein_id="HCD1932759.1"
                     /translation="MSKHNTDTSDQHAAKRRWLNAHEEGYHKAMGNRQVQMIAIGGAI
                     GTGLFLGAGARLQMAGPALALVYLICGLFSFFILRALGELVLHRPSSGSFVSYAREFL
                     GEKAAYVAGWMYFINWAMTGIVDITAVALYMHYWGAFGGVPQWVFALAALTIVGTMNM
                     IGVKWFAEMEFWFALIKVLAIVTFLVVGTVFLGSGQPLDGNTTGFHLITDNGGFFPHG
                     LLPALVLIQGVVFAFASIEMVGTAAGECKDPQTMVPKAINSVIWRIGLFYVGSVVLLV
                     MLLPWSAYQAGQSPFVTFFSKLGVPYIGSIMNIVVLTAALSSLNSGLYCTGRILRSMA
                     MGGSAPSFMAKMSRQHVPYAGILATLVVYVVGVFLNYLVPSRVFEIVLNFASLGIIAS
                     WAFIIVCQMRLRKAIKEGKAADVSFKLPGAPFTSWLTLLFLLSVLVLMAFDYPNGTYT
                     IAALPIIGILLVIGWFGVRKRVAEIHSTAPVVEEDEEKQDIVFKPETAS"
BASE COUNT         6993 a         6763 c         7001 g         6806 t
ORIGIN      
        1 tgtaaattct tccagccgga gtgaatgcgg ttacctacac tccagattac tgaccactgg
       61 aggcagacac tatggcgaac agcatcacgg cggatgagat tcgggaacag ttttcgcagg
      121 caatgtcagc catgtaccag caagaagttc cgcagtacgg cacgctgctg gaactggtag
      181 ctgatgtaaa tctggcggtg ctggaaaaca atcctcaact gcacgaaaaa atggtaaatg
      241 cagacgaact ggcgcgactg aatgttgaac gtcatggggc gattcgcgtt gggactgcac
      301 aagagcttgc tactcttcgg cggatgtttg ccattatggg gatgtacccg gtgagctatt
      361 acgatctctc gcaggcaggg gtgccggtac attcgacagc atttcggccc attgatgctg
      421 cttctctggc gcgtaatccc ttccgcgttt ttacctcatt actccgcctt gagcttatcg
      481 agaacgaatt attacgccag aaagcggcgg agattctgcg tcagcgcgat atcttcaccc
      541 cacgttgtcg acaactgtta caggaatatg agcagcgggg cggttttaac gaaacacagg
      601 cacaggagtt tgtgcaggaa gccctggaaa cgtttcgctg gcaccagtca gcaacggtag
      661 atgaaaaaac ctatcgcgcc ttgcacaacg aacatcggtt gattgctgat gtggtctgtt
      721 ttcctggatg ccatatcaac cacctgacgc cacgcacgct ggatattgac cgggtgcagt
      781 cgatgatgcc tgaatgcgga attgaaccca aaattctgat cgaagggccg ccgcgccgcg
      841 aggtgccgat tttactacgc cagaccagct ttaaagcact ggaagagacg gtgttgtttg
      901 cggggcagaa acagggcaca cataccgcgc gctttggtga aattgagcag cgtggtgtgg
      961 cattaacgcc gaaagggcga caactgtatg atgatcttct gcggaacgct ggaaccgggc
     1021 aggataatct cactcaccaa atgcatttac aggaaacctt ccgcactttt cctgacagtg
     1081 agtttttaat gcgtcagcaa gggctgggat ggttccggta ccgtctgacg ccttcgggtg
     1141 aggcgcatcg tcaggcgatt catcccggag acgatccaca gcccttaata gaacgtggtt
     1201 gggtagtggc gcaacccatt acctatgaag atttcttgcc cgttagcgcg gcggggatct
     1261 tccagtcaaa tctgggtaat gaaacgcagg catgcaatca cggtgatgcc agtcgcgaag
     1321 catttgagca ggcgttgggg tgtccggttt tggatgagtt ccagctttat caggaagcgg
     1381 aagaacgcag taaacgtcgc tgtggtttgc tttaaattct gaccatccgc ctttgcaaaa
     1441 atttgcctga ttttacaaac gaatcaggct catcccatcg acataaaaaa aatgccaatt
     1501 tatgcatatt ctctgatttc aacaattgga ttattaataa atattgtcta gagtgagcgg
     1561 tcataaataa gcactttctt gccgctgaaa atgaccagcg cgggaccatt cacaacacca
     1621 gaaggactca ctttcaggta tggatcgtag acgatttatt aaaggttcaa tggctatggc
     1681 cgccgtgtgc ggtaccagcg gcattgcttc tcttttttct caggcggcat tcgcggccga
     1741 ttctgatatt gccgacgggc aaacccagcg ttttgacttc tccattctac agtcaatggc
     1801 gcacgactta gcgcaaacag catggcgtgg tgcaccacgt ccgttacctg atactctggc
     1861 gacaatgacg ccgcaggctt ataacagtat tcaatacgac gccgaaaaat cgctctggca
     1921 taacgttgag aaccgtcaac tggacgctca gtttttccat atgggaatgg gattccgtcg
     1981 ccgcgttcgt atgttttctg tagatccagc aacacatctg gcgcgtgaaa ttcactttcg
     2041 cccggagttg ttcaaataca acgatgcggg tgtggatacc aaacaattag aagggcaaag
     2101 cgatctcggt tttgccggtt ttcgcgtgtt taaagccccc gaactggcgc gccgtgatgt
     2161 agtatcattc ctcggcgcga gttatttccg cgccgttgat gacacatatc aatacggttt
     2221 gtcggcccgc ggcctggcga tcgacactta caccgacagc aaagaagagt tccccgactt
     2281 taccgccttc tggtttgata cggtaaaacc aggggcaacc acctttaccg tttatgcgtt
     2341 gctcgatagc gccagcatta ctggtgccta taagttcact atccattgtg agaaaaatca
     2401 ggtgattatg gatgtggaaa atcacctgta tgcgcgcaaa gacattaaac agctgggcat
     2461 tgcaccgatg accagtatgt tcagctgcgg tactaatgaa cgtcggatgt gcgacaccat
     2521 tcatccgcaa atccatgact ctgatcgtct gtccatgtgg cggggcaacg gcgagtggat
     2581 ttgccgtccg ctgaacaatc cgcaaaaatt gcagttcaat gcttacaccg acaacaaccc
     2641 gaaagggttt ggtttattgc aactggatcg tgatttctcc cattatcagg acattatggg
     2701 ctggtataac aaacgcccaa gtctgtgggt ggaaccgcgt aacaagtggg gtaagggcac
     2761 catcggcctg atggaaatcc caactacggg ggaaacgctg gataacattg tttgcttctg
     2821 gcagccagaa aaagctgtaa aagcgggtga tgagtttgca ttccagtatc gtctgtactg
     2881 gagtgcgcaa ccgcctgttc attgcccatt agcgcgcgtt atggcgacgc gtaccggcat
     2941 gggcggtttc ccggaaggtt gggcgccagg tgaacactat cccgaaaaat gggcgcgacg
     3001 ttttgccgtc gatttcgttg gtggcgatct gaaagctgcc gcgccaaaag gcattgagcc
     3061 ggtgattacg ctttccagtg gggaagcgaa gcaaatcgaa attctctata ttgaacccat
     3121 tgatggttat cgtattcagt ttgactggta tccgacttcg gactccactg atccggtcga
     3181 tatgcggatg tatctgcgtt gtcagggcga cgctatcagt gaaacatggt tatatcagta
     3241 tttcccgcca gcgccggata aacgtcagta tgttgatgac cgcgtgatga gttaatcgtt
     3301 ttttcttcgg catcttcttc gggaggtgcc gtctggttaa acacgatccc gctcgcattt
     3361 tttcctaagt taaatgagta atctgctggt gtgtatttca gatacacctt gtcagccact
     3421 aacagggagt gcgtatgttt ccagaatacc gagatttaat atcccgtctg aaaaacgaaa
     3481 atcctcgctt tatgtccttg ttcgataaac acaataaact tgatcatgaa attgccagaa
     3541 aggaaggttc cgacggtcga gggtacaatg cggaagtggt ccgcatgaaa aaacaaaagc
     3601 tacagttaaa agatgagatg ctcaaaatcc tgcagcaggt gagcgtcaaa gaggtgtaaa
     3661 ctttcctaag ccgccagcca ggcggctttt ttaacagctg catggattga ctggagataa
     3721 gatgactgaa acgataaaag taagcgaatc acttgaatta catgctgttg cggaaagtca
     3781 tgtcacacct ctttatcagt taatctgtaa aaataaaacc tggttacagc agtcgctaaa
     3841 ctggccgcag tttgttcaaa gtgaagagga cacgcgaaaa acggtgcagg gtaatgtgat
     3901 gttgcatcaa cgcggctatg ccaaaatgtt catgattttc aaagaagatg aacttatcgg
     3961 cgttatctct tttaatcgta ttgaaccact gaataaaacc gctgaaatcg gctactggct
     4021 ggacgaacct catcaggggc aggggatcat ttctcaggcg ctgcaggcat tgattcatca
     4081 ttacgctcag tctggtgaac ttagacgctt cgtgatcaaa tgtcgggtgg acaatccgca
     4141 aagcaaccag gtagcgttgc gcaatggttt tatccttgaa ggttgcctga aacaggctga
     4201 gtttctgaat gatgcctatg atgatgtgaa cttatacgcg cgtattatcg attcacaata
     4261 accctgccag cagcgttcgc gtaatgcgct cctcgccgtt aatgactttt ggaccacgga
     4321 catgaatcgt gtcgccatca aaggcttcaa cgacggcgtg gtcagttaac tccacatggt
     4381 tttcaattat caccgcacct gtaatacggc tctcaccttg tattacaacg tgctcgtcga
     4441 gcagaatcgg tccgccacgt accacggcat ttccgccaat caagacatga tgttttaaca
     4501 cacaattacc ttccacaatg gcatattccg ccacctgtga actgtaatga atcgtcggaa
     4561 tggcatcttc ttctatgcca gctttcacct gcgcgtgacc gtaaacttta gcgcaatcgc
     4621 atagccagac gttgttctct tcattacctt caatactggc aaaatcaaaa acttcggcgc
     4681 gatgttcaat aaaagcatac cggacgacgg catcgccata aatttgtgcc tggtggacaa
     4741 tacgcgaggc gctaaccttt gcgcgatcat aaatttgtag caggagttga tggtcgggcg
     4801 ttaagccttg tgcggcgacg atcatagaat gttgatcaat taaggcgtga ccaaatattc
     4861 tacattgacc gcaaactaat gagtcactaa tggtgacact gtcacttata tatgcaccct
     4921 ggctgatttc actattatcg atccagacat tatccgttgc gtaaacctct ccccacagca
     4981 cgctggtgcc ggtgatacgc gtattcccgg aaatcaccgt tccgccgaac gcaatagcat
     5041 tctggtcgta aatccagcaa tttccttcct gtgctaacac cgtttcgcga tcgatccagc
     5101 cgccagcgct tcctgctatc acatcgttaa aatcgctcat ggcgataatt tgccgtaata
     5161 acacactttt tttagtgcca tcttcctgat aactaaaggc tcgctgttct tcacttagac
     5221 gatatttacg catagcgttt tcccacaggt gacttactat aaccgtagca aattctgcgg
     5281 ctctggctat gctcgagaaa attccataaa atgcatttca aatatacttt ataaattaaa
     5341 caaaatgagt aagaagatgc agagcgataa agtgctcaat ttgccggcag gctactttgg
     5401 tattgtgttg gggacgatag ggatgggatt tgcctggcgc tatgccagcc aggtttggca
     5461 ggtcagccac tggttggggg atgggctggt gattctggcg atgatcatct ggggattatt
     5521 gactggcgca tttattaccc gactcatacg ctttccgcat agcgtgctgg cagaagtgcg
     5581 ccatccagtg ctgagcagtt ttgtgagttt gtttccggca acgacgatgc tggtggcgat
     5641 tggttttgtt ccgtggtttc gcccactggc ggtgtgcctg ttcagttttg gtgtcgtggt
     5701 tcagttggct tatgccgcct ggcaaactgc gggattatgg cgtggatctc accctgaaga
     5761 agctaccacg cctggactgt atctgccgac agttgccaac aactttatca gcgcaatggc
     5821 ctgtggtgcg ttgggctaca ccgacgccgg tctggtgttt ttaggcgcag gcgttttctc
     5881 atggctaagc ctggaaccgg tgatcttgca gcgtctgcgc agttcgggag aattacccac
     5941 ggcactgagg acatcactcg gcattcagct cgctcctgcg ctggtggcct gtagtgcctg
     6001 gctgagcgtc aacggcggcg agggtgacac gctggcgaaa atgcttttcg gttatggact
     6061 gctgcaactg ctgtttatgc tacgtctgat gccatggtat ctctcccagc catttaatgc
     6121 ttcattctgg agtttctcgt tcggcgtatc tgcactggca accaccggtt tgcatctggg
     6181 gagtggcagc gataatggat ttttccatac gctggcggtg ccgctgttta tctttaccaa
     6241 ttttattatt gcaatactgc tcatccgtac ttttgcgctt ctgatgcagg gaaaattgtt
     6301 agtcagaacc gagcgcgccg ttttaatgaa agcagaggac aaagaatgat cattcgtgat
     6361 gaaaactatt ttactgataa atatgaatta acccgcacac actctgaagt gctggaagcg
     6421 gtgaaagtgg ttaaaccggg taaaacgctg gatctgggct gtggcaatgg tcgtaacagt
     6481 ctttacctgg cagccaatgg ttatgatgtt gacgcatggg ataaaaatgc catgagtatc
     6541 gccaacgtcg agcgcattaa gtccattgaa aatctggata atttacacac ccgggtcgtt
     6601 gatctgaata acctcacatt tgatggacag tacgatttta ttctttcgac tgtggtgctg
     6661 atgttccttg aggctaaaac catcccaggg ctgattgcca atatgcaacg ttgcgctaaa
     6721 cctggcggtt acaacctgat tgtggcggcg atggataccg ctgattatcc atgtaccgtc
     6781 ggcttcccgt ttgccttcaa agagggggaa ttacgtcgat attacgaagg ctgggagatg
     6841 gtgaaataca atgaagacgt cggcgagctg caccgcaccg acgccaacgg taatcgtatt
     6901 aaactgcgtt tcgccacgat gctggcacgt aaaaaatgat ccggtaagaa caaagcgcgt
     6961 gaaaattccc cacgctgaga tggtttactg ttcttctttt cggtaagcat attttttatc
     7021 gaagggatgt gaaattaatc acagtagtcg aagtttttta gccgcttaac gtactgaaat
     7081 ttaactactg atgattgact tagccccttt ttcggcattg actatgtcgt ctgaaaaggg
     7141 gctaaaaaat ttattttcac caacactttt tttgccacaa cacgaagcgg cgctttttgc
     7201 tataacttaa aaagtaatat aatcatctca ggaaactatt catgcgtacc acatcatttg
     7261 cgaaagttgc agctttatgc ggcttgttgg ctctgtctgg ttgtgcctct aaaatcaccc
     7321 agccagataa atattctggt tttttaaaca attactctga tttaaaagaa acaacctcgg
     7381 caacaggtaa acctgtttta cgttgggtag acccgagttt tgatcaaagc aaatatgaca
     7441 gcatcgtctg gaacccaatc acttattatc cggtaccgaa accttctacc caggtagggc
     7501 agaaagttct ggataaaatt ttgaactata ccaacaccga aatgaaagaa gcgatagcgc
     7561 agcgtaaacc actggttacc accgctgggc cgcgtagtct gattttccgt ggggccatta
     7621 ccggtgtaga taccagcaaa gaagggctgc aattctatga agtggttcct gttgcattag
     7681 tggttgcggg gacgcaaatg gctacaggcc accgtaccat ggatactcgc ttgtattttg
     7741 aaggtgagct gattgatgca gcgactaata aaccggttat caaagtcgtt cgtcagggcg
     7801 aaggtaaaga cctgaataac gaaagtacgc caatggcttt cgaaaatatt aaacaagtta
     7861 ttgatgacat ggcgaccgat gccaccatgt ttgacgttaa caaaaagtag ttccagacgc
     7921 gccatcgtta gatggcgctt tttattatcg gtttctgtta gcgattaaat tcaacaccac
     7981 gtaacaaacg cctccggcaa ttaatcccca aaacgcagaa ccaatcccga acagcgtcaa
     8041 tccacttgcc gttaccagaa atgccaccac cgccgcgtcg cgctcacgct cattatgcag
     8101 cgcctgatac aaactgccgc caatggtact caacagcgca agacctgcca gcatctggat
     8161 ccaacttacg ggcagggcag ccatcatccc ggtaatggca ctaccaaaca gacctgcgat
     8221 caaataaaaa atgccggcaa cggcagcggc cagccaacgt tgatctttat ccggatgcgc
     8281 ttccgggctt tggcaaatag ccgcggtgat tgccgcaata ccgacggaat aaacgccgaa
     8341 aggggaaaaa aacagtgcca gcaatccagt aaacacaatc aacggcgaaa caggagccga
     8401 atacccggct gctttcattg ctgcgatacc cggtgcgttt tgcgatgcca tcgtcaccag
     8461 aaaaaggggg agtgcaacgc tcagactgtg agcaaacgaa aaatcagggg taatataagt
     8521 ggggagaacg ggtttaaaga caatatcagt tgtaacaacg tcaccttgcg cggtaacgat
     8581 gacgatccca ataatcatcg cggcaattac cgcatagcgc ggcgcaacgg ccttggctgc
     8641 cagccatgcc agcaacatac tgccacacaa cgtaaattga ccatccagac tggcaaacgc
     8701 ctgtaaacca aaacgtaata aaatcccggc aagcattgcc gccgcaagcg agtgcggaat
     8761 aatgcgcatc agacgagcaa agagtcccgt tatgccgcag aggactatta acgcgttggt
     8821 gacaataaaa acgccgatgg cttcgttaag tgttagttcc tgcaatccgg tgaccaacaa
     8881 agccgcgcca ggcgttgacc atgcggtgag aacaggtacg cgataccata atgtcagagt
     8941 cagcgtactg acgcccattg ccagccccag cgccgtcatc cagccagaga tttgtgcagt
     9001 ggtggctccg gcgacaatcg ctgcttgcca gattattgcc gctgaactgg cgtagccaat
     9061 taataccgcc agaaaccccg ccagtagcgt gggtggagga atagaaaaca gacgcatagt
     9121 cacctcgtgc gttataacgt acaggtaccg tagcatttgt ccgttataac gcacaagtga
     9181 taaacttccg ttttgccgga ggagtcgcat ggaaaatctc gctcgttttt tatccaccac
     9241 acttaaacaa ctacgtcagc agcgcggctg gagtctttcg cgactggcag aagcgacggg
     9301 cgtttctaaa gcgatgttag ggcagattga gcgtaatgaa tccagcccga cggtcgcgac
     9361 attatggaaa attgccaccg ggttgaacgt gccattttca acatttattt ctccgccgca
     9421 gtccgctacg ccttcagttt atgatccaca acggcaggcg atggtgatca cttcgctgtt
     9481 tccttatgat ccacaactct gctttgaaca cttctcgatt cagatggcac ccggtgcaat
     9541 aagcgaatcg atgccccatg aaaagggggt aatcgaacat gtcgtcgtca ttgatggaca
     9601 acttgatctg tgtgttgatg gtgaatggca gagccttaat tgtggggaag gcgttcgatt
     9661 tgctgcagac gtcacgcatg tctatcgtaa tggtggggag caaaccgtac attttcattc
     9721 cctcatccat tacccgcgca gttaagcggg aaaactattt cgcaacgtcg cgcttgtggc
     9781 taaaatagcc gccatttttc agctactgga taagaaagtg accgtatctt ctcatcgtct
     9841 tgaactgtta agcccggcac gcgatgccgc cattgcccgc gaagctattt tgcacggtgc
     9901 cgatgctgtt tatatcggcg gccctggttt tggtgcccgt cataatgcca gtaatagcct
     9961 gaaagatatt gccgagttgg tgccgtttgc ccatcgttat ggtgcaaaaa ttttcgtcac
    10021 gcttaacacc attttgcatg atgatgagct ggaacccgcg caacggctga ttactgacct
    10081 ctaccagacc ggtgtcgatg cgctgattgt tcaggatatg gggattctgg aacttgatat
    10141 tccgccgatt gaactgcacg ccagtacgca gtgcgatatt cgtacagtag aaaaagcgaa
    10201 gttcctctct gatgttggct tcacgcagat tgtgctggcg cgagaactga atcttgagca
    10261 gatccgcgcg attcaccagg ccacgaacgc gaccattgaa ttctttattc atggcgcatt
    10321 gtgcgtggcc tattcgggtc agtgctacat ttctcatgcg caaacagggc gtagcgccaa
    10381 ccgtggcgat tgctcgcagg cgtgccgttt gccatacaca ttgaaagacg atcaggggcg
    10441 ggtggtttcc tatgaaaaac atctgctgtc gatgaaagat aacgatcaga ctgccaacct
    10501 cggcgcgctg attgatgctg gcgtacgctc cttcaagatt gaagggcgtt acaaagatat
    10561 gagctacgtg aagaatatca ccgcccatta tcgccagatg ctggatgcca ttattgaaga
    10621 acgtggcgat ctggcgcgcg cttcatcagg tcgtactgaa catttctttg ttccatcgac
    10681 ggaaaagact ttccaccgtg gtagcacaga ttattttgtg aatgcccgta aaggcgatat
    10741 tggcgcgttc gattcgccga aatttatcgg cctgccggtg ggcgaagtat tgaaagtagc
    10801 gaaagatcat attgatgttg ccgttaccga gccactggca aatggcgatg gcctgaacgt
    10861 gatgattaaa cgtgaagtcg tcggttttcg tgccaatacg gtagagaaaa ccggagaaaa
    10921 tcagtaccgc gtctggccaa atgaaatgcc agcagatttg cacaaaattc gcccgcatca
    10981 cccactaaac cgtaatcttg atcataactg gcaccaggca ctgacaaaaa cctccagcga
    11041 acgtcgggtg gcggtagaca ttgaactggg cggctggcag gaacaactga ttctgaccct
    11101 gaccagtgaa gagggcgtca gcgtcacgca tacgctggac gggcagttcg acgaagccaa
    11161 taacgccgaa aaagcaatga acaatctgaa ggatggtctg acaaaactgg ggcaaaccat
    11221 ctattacgcc cgcaatgtgc aaattaattt gccgggggcg ctgtttgtac caaacagtct
    11281 gttaaaccag ttccgccgtg aagccgccga tatgcttgat gctgcgcgtc ttgccagtta
    11341 ccagcgcggc agccgtaaac cggttgctga tcctgcgccg gtttatccgc aaacgcatct
    11401 gagtttcctc gcgaacgtat acaaccagaa agcgcgtgaa ttttatcatc gctatggtgt
    11461 gcaactgatt gacgcggcgt atgaggcaca tgaagagaag ggcgaagtcc cggtgatgat
    11521 caccaagcat tgtctgcgct ttgccttcaa tctgtgcccg aaacaggcga aaggcaatat
    11581 caaaagctgg aaggcgacgc caatgcaact ggttaacggc gatgaagtat taacgctaaa
    11641 gtttgattgc cgcccatgcg agatgcacgt cattggcaaa atcaaaaatc acatactgaa
    11701 aatgccgtta ccgggaagcg tagtggcatc cgtaagtccg gatgatctgc tgaaaacatt
    11761 gccgaagcga aaagggtaaa acgccagttt tcgggttact caccactcat caaatctgca
    11821 tgatattgcc tgccgggtaa ggcgtcatgc cgcatccggc aacaaatgac tcaacgctgt
    11881 cccgcttctg gtttgcgcga tttttgccag taaaaatgct cgcgcaaacc ttccgccgac
    11941 tcttccgcca cagcacgcaa ttcatcgctg tccgcttcat gacgcagctg atgatccaca
    12001 ttctttaccc acataaattc atgtcctttg tgccctgcca tgagttgtcc tgagaacagt
    12061 gcacacgtta ataagacaac cgataacgcc ttcgaaaaca tcccgccacc tttttctaac
    12121 cttttgccgc tatgatgccg atcgtttctt gaggttatta ttcagttttg caaattagag
    12181 caaagaaatt ctggaatctt ccttcctgat tttgcattga attctgccgt tgcggcgatt
    12241 tagtgctatt ttcgagcaca ttacacacgg aggtaaactt cgccattaaa tagtcacgat
    12301 cactttattg aagtgccttt gagcgtcgcc tctaatgtat tgctgttaaa tgctttttta
    12361 cagtcagaaa tcactcagca agagttagcc aggcgaattg gcaaacataa acaggagatt
    12421 actcgcctat ttaatttgca ccatgcgaca aaaatcgacg ccgtccagct cgcggctaag
    12481 gcgcttggca aagagttatc gctggtgatg gtttaattac agttaacgaa aagttgtcat
    12541 ttttaacaac tgatatagac tgccgaatca tctgcacata attacgattc gataatgaaa
    12601 aaataccagc agcttgcaga acaattacgc gagcagattg cgtcgggtat ctggcaaccc
    12661 ggcgatcgtt tgccttcgtt gcatgaccag gtggcgcttt ctggcatgag ctttatgact
    12721 gtcagccatg cctatcagtt gctcgaaagt cagggatata ttatcgcgcg accgcaatcg
    12781 ggttattacg ttgcgccaca ggcaataaaa atgccgaaag cgacagtcat tccagtcaca
    12841 cgagatgaag cagtcgatat caacacctat atttttgata tgttgcaggc cagtcgcgat
    12901 ccgtcggtag ttccgtttgc ctcggccttt cccgacccgc gacttttccc cctccaacaa
    12961 ctaaaccgct cgctggcgca ggtaagcaaa accgccacgg cgatgagcgt gattgaaaac
    13021 ttaccgccag gaaacgcaga actgcgtcag gctattgctc gtcgctatgc cttacagggc
    13081 atcaccattt ctcctgatga aattgtcatt accgccgggg cgttagaggc attaaacctc
    13141 agtttgcaag cggtaactga accgggcgat tgggtgatag tagagaatcc ttgtttctac
    13201 ggcgcgttgc aggcgctgga gcggttacgg ctgaaggcgt tatcggtagc gacggatatt
    13261 aaagaaggga tcgatcttca ggcgctggaa ctggcgttgc aggagtatcc ggtgaaagcg
    13321 tgctggctga tgactaatag ccagaaccca cttggattta ccttaacgcc gcagaaaaaa
    13381 gtgcggctgg tagcgttgct caatcagtac aacgtaacgc tgattgaaga tgacgtttat
    13441 agcgaacttt attttggacg ggaaaaaccg ctgcctgcga aagcgtggga tcgccacgat
    13501 ggtgttttgc attgctcttc gttttcgaaa tgtctggtgc ctggttttcg tattggttgg
    13561 gtcgccgccg gaaaacatgc acgtaaaatt caacgcttgc agttgatgag tacgctttcc
    13621 accagctcac cgatgcagct tgcgctggtg gattaccttt ctactcgccg atacgatgct
    13681 catcttcgtc gcctgcgtcg ccagcttgcg gaacgtaaac aacgcgcctg gcaggctttg
    13741 ctgcgttatc tgcctgcgga agtcaaaatt catcacaatg acagcggtta ttttctctgg
    13801 ctggagctcc ccgagccgtt agatgcagga gaattaagcc tggtggcact gacgcatcat
    13861 atcagtattg cgccgggtaa tatgttttct accggtgaaa actggtcacg tttttttcgt
    13921 tttaataccg cgtggcagtg gggagaacgt gaagaacagg cggtaaaaca attaggcaaa
    13981 cttattcaag aacggctgta atagcgttta atttaattcc tctcaggttg ggtaatatga
    14041 atttcgaata gcagtcatat atcctaactc cttgtctata ctccagaaga taaccttaca
    14101 gacggcataa tgcgcggtag ctcacaacct gattaaattt tctcagggcc gaaggtgtgc
    14161 ctgcaagccg ccgtctatgg ttaaacaagg agatattttt acggcacggc ggctgaacaa
    14221 ttaattacga caggagtaag accttatgag caagacattt gcccgcagca gcctgtgtgc
    14281 gctcagcatg acaataatga ccgctcacgc cgccgaaccg cctaccaatt tagagaatcc
    14341 ggaagggcga ctggatatta tcgcctggcc gggatacatc gaacgcggac aaactgataa
    14401 acagtacgac tgggtaacgc aattcgaaaa agagacaggc tgcgcggtga atgttaaaac
    14461 cgccgcgact tccgatgaaa tggtcagtct gatggccaaa gggggttacg atctggttac
    14521 ggcatccggc gatgcctcgc tgcgtttgat tatgggcaaa cgcgtgcagc caattaatac
    14581 cgccttgatt cccaactgga aaacgctcga tccgcgcgtg gttaaaggcg actggtttaa
    14641 cgttagtggc aaagtttatg gcacacctta ccaatggggt ccgaacctgt tgatgtacaa
    14701 cactaaaacc ttcccgacgc cgccggatag ctggcaagtg gtttttgttg agcagaatct
    14761 gccggacggc aagagcaata aaggccgcgt tcaggcttat gatggcccta tctacattgc
    14821 ggacgctgcg ttgttcgtta aagccactca gccgcagttg ggcatcagtg atccgtatca
    14881 actcaccgaa gaacagtacc aggcggtgct gaaagtgctg cgcgatcaac atagtttgat
    14941 ccatcgctac tggcatgaca ctaccgtgca aatgagcgat ttcaaaaacg agggtgtggt
    15001 tgcttccagt gcatggccct atcaggccaa cgccctgaaa gccgaaggcc agcctgtcgc
    15061 taccgttttc ccgaaggagg gcgttaccgg ttgggctgac accaccatgc tacatagcga
    15121 agcgaaacat ccggtttgcg cctacaaatg gatgaactgg tcattaaccc caaaagtgca
    15181 gggcgatgtg gcggcctggt ttggctcgtt accagtagtg ccgcaagggt gtaaagccag
    15241 tccgttatta ggcgagaaag gttgtgaaac aaacggtttt aactatttcg ataaaatcgc
    15301 cttctggaaa acgcctgtag cagaaggggg caagtttgtt ccctacagtc gctggacgca
    15361 ggattacatt gccattatgg gcggtcgcta acttcgctgg ggtgttttat gacgtacgca
    15421 gtggagtttg acaacgtctc acggttgtac ggtgacgtgc gcgcagtaga tggcgtcagt
    15481 attgcgataa aagatggtga gttcttctct atgctggggc cgtccggctc cggcaaaacc
    15541 acttgcctgc gcctgattgc tggattcgaa cagctttccg gcggggctat ctctattttt
    15601 ggtacgcccg ccagcaatct gccaccgtgg gagcgggacg tgaatactgt cttccaggac
    15661 tacgcgctat ttccgcatat gtcgattctt gacaacgtag cctacgggct gatggtcaaa
    15721 ggcgtgaata aaaagcagcg gcatgcaatg gcgcaagagg cgcttgagaa agtggcgttg
    15781 gggtttgtac agcaacgtaa accgtcacaa ctttctggtg gtcagcgcca gcgggttgct
    15841 atcgccagag cactggtgaa cgaaccgcgt gtgttgttgc tggatgaacc gctcggcgcg
    15901 ctggatctca aattgcgaga gcaaatgcag cttgagctga aaaagctgca acagtctctc
    15961 ggtatcactt ttatcttcgt tacccacgat cagggcgaag cgttatcgat gtccgatcgg
    16021 gtggcggttt tcaataatgg acgcattgaa caggtcgatt ctccgcgcga tctctatatg
    16081 cgcccgcgca cgccgtttgt tgcgggattc gtcgggacat ccaacgtttt tgatccgcta
    16141 atggccgaga aactttgtgg catgacggga agcttcgcac tacgaccgga acatatccgc
    16201 ctcaacaccc atggcgaaat gcaggctaat ggcacgatcc aggcggtgca atatcagggc
    16261 gcagcgaccc gttttgaact gaaattgaac ggcggtgaaa aactgctggt gagtcaggcc
    16321 aatatgacag gcgaagagct acctgccacg ctcacgcccg gacaacaggt gatggtttcc
    16381 tggtcacgtg atgtgatggt ggcgctggtt gaggagaggt gaatggcgat gaacgtattg
    16441 caatcacctt cacgtccagg tctgggtaag gtgtccggtt tcttctggcg taatccgggg
    16501 ctggggctgt ttttgctgct gcttggcccg ctaatgtggt ttggcattgt ctatttcggc
    16561 tcgctgctga cgctgttatg gcagggcttt tatacctttg acgatttcac catgtcggta
    16621 acgccggaac tgacgctggc gaatatccgc gcgctgttta atccggcgaa ttacgacatc
    16681 attgtccgca cgctgaccat ggctgtggca gtcactatcg ccagcgccat tctggctttt
    16741 ccaatggcgt ggtatatggc gcgctatacc agcgggaaaa tgaaagcgtt tttttatatc
    16801 gcggtaatgt tgccgatgtg ggcgagctac attgttaaag cctatgcctg gacgttattg
    16861 ctggcaaaag atggcgtggc gcagtggttt ttacaacatc tggggctgga accactgctg
    16921 actgcgttcc tcacgctacc ggcggtggga ggtaatacgc tgtcaacttc cgggctgggg
    16981 cgctttctgg tgtttctcta tatctggttg ccgttcatga tcctcccggt gcaggcggcg
    17041 cttgagcgtt tgccgccgtc attgttgcag gcgtcggctg atcttggcgc acgtccacga
    17101 caaacctttc gctatgtggt gctgccgctg gcaatcccgg gtattgccgc tggctctatc
    17161 tttaccttct cactcacgct gggcgatttt attgtcccgc agctggttgg ccctccagga
    17221 tattttatcg gcaatatggt ttattcccag cagggggcga ttggcaatat gccgatggcg
    17281 gcggcattca ccctggtgcc gattgttctc atcgcacttt acctggcgtt cgtgaaacgt
    17341 ctgggagcgt tcgatgcact ctgaacgcgc accgtttttc ctcaaactgg cggcctgggg
    17401 cggcgtggtt ttcctacatt ttcccatcct gattatcgct gcctatgcgt ttaacactga
    17461 agatgcggcg tttagttttc caccgcaagg cctgacgctg cgctggttta gcgtggcagc
    17521 acagcgtagt gatatccttg atgccgtgac actgtcactt aaagtggcgg cgctggcgac
    17581 attaatcgcg ctggtgttag ggacgctggc agctgccgcg ctgtggcgac gagacttttt
    17641 cggcaaaaac gccatttcgc tgttactgtt gctgcccatt gcgctgccgg gcattgtcac
    17701 tggtctggcg ctattaaccg cctttaaaac catcaatctg gagccaggat ttttcaccat
    17761 cgtggtcggt catgcgactt tttgcgtagt ggtggtgttt aacaatgtca tcgcccgttt
    17821 tcgccgcacc tcgtggagtc tggttgaggc gtcaatggat cttggggcca atggctggca
    17881 aaccttccgc tacgtagtgt tgccgaatct cagttcggcg ttactggctg gaggaatgct
    17941 ggcgtttgcc ttgtcgttcg atgaaatcat cgtgacgact tttacggcag gtcatgaacg
    18001 aacgttaccg ttgtggttgc tcaatcagct tgggcgaccg cgtgatgtac cggtaaccaa
    18061 cgtggtggca ctgctggtta tgttggtaac aaccttgccg atcctggggg cctggtggct
    18121 aacccgcgaa ggcgacaatg gtcaataacc actgatacag gaatatgcta tgcaacataa
    18181 gttactgatt aacggagaac tggttagcgg cgaaggggaa aaacagcctg tctataatcc
    18241 ggcaacgggg gacgttttac tggagattgc cgaggcatcc gcagagcagg tcaatgctgc
    18301 tgtgcgcgcg gcagatgcag catttgccga atgggggcaa accacgccaa aagcgcgtgc
    18361 ggaatgcctg ctgaaactgg ctgatgttat cgaagaaaat ggtcaggttt ttgccgaact
    18421 ggagtcccgt aattgtggca aaccgctgca tagtgcgttc aatgatgaaa ttccggcgat
    18481 tgtcgatgtc tttcgctttt tcgccggtgc ggcgcgctgc ctgaatggtc tggcggcagg
    18541 tgaatatctt gaaggtcata cttcgatgat ccgtcgcgat ccgttggggg tcgtggcttc
    18601 tatcgcaccg tggaattatc cgctgatgat ggctgcgtgg aaactggctc cggcgctggc
    18661 ggcagggaac tgcgtagtgc ttaaaccatc agaaattacc ccgctgaccg cgttgaagtt
    18721 ggcagagctg gcgaaagata tcttcccggc tggcgtgatt aacgtgctgt ttggcagagg
    18781 caaaacggtg ggcgatccgc tgaccggaca tcccaaagtg cggatggtgt cgctgacggg
    18841 ttctatcgcc actggggaac acatcatcag ccataccgcg ccgtccatta agcgtactca
    18901 tatggaactt ggtggcaaag cgccagtgat tgtttttgat gatgcggata ttgaagctgt
    18961 ggtcgaaggt gtacgtactt ttggctatta caatgccgga caggattgta ctgcggcttg
    19021 tcggatctac gcgcaaaaag gcatttacga tacgctggtg gaaaaactcg gtgctgcggt
    19081 ggcaacgtta aaatcgggtt cgccggatga cgagtctacg gagcttggac ctttaagctc
    19141 gctggcgcat ctcgaacgcg tcagcaaggc ggtagaagag gcgaaagcga cagggcacat
    19201 caaagtgatc actggcggtg aaaagcgcaa gggtaatggc tattactatg cgccgacgct
    19261 gctggctggc gcattacagg atgatgccat cgtgcaaaaa gaggtatttg gtccggtagt
    19321 gagtgttacg ctcttcgaca gcgaagaaca ggtggtgaac tgggcgaatg acagtcagta
    19381 cggacttgca tcttcggtat ggacgaaaga tgtgggcagg gcgcatcgcg tcagcgcgcg
    19441 gctgcaatat ggttgtacct gggtcaatac ccatttcatg ctggtaagtg aaatgccgca
    19501 cggtgggcag aaactttctg gttacggcaa ggatatgtca ctttatgggc tggaggatta
    19561 caccgtcgtc cgccacgtca tggttaaaca ttaaggataa tattgcagat cgtaagagta
    19621 tgagatgatc ttgcgtactg tcagagcact gatttctctg acagtacgtg ctgcgtgcaa
    19681 aatcactaca tcaaataaac cagccaaatc tcatagagat aagagtaaac aaaccaacag
    19741 cagcaaagac atttatcagt actacagttc tactggaaac attcattttt gccacctttt
    19801 atcattgccc ctatgtgtat agtacgggtt aagaaaatcc gcaataatac tcattctgaa
    19861 gatggataag ggcaagttgc tgtttgatga ttttttctca aactggtgaa aaaaacttga
    19921 tgcacgtcaa aaaatgacgc atatttgcgc gcgttttatt catctggctg gacgcccgta
    19981 catgtctctc tatcaacaca tgcttgtttt ctatgcggtt atggcagcaa tcgcatttct
    20041 tatcacctgg tttctttctc atgataagaa acgcatccgt ctcttaagcg cctttctggt
    20101 gggggcaaca tggccgatga gtttcccggt ggcgctgttg ttttctctgt tttaaatctt
    20161 atgcactggc ttgacggcac cacgcagact tatatcattt ggatgaatcg ataaatttca
    20221 caagtggcta aggagaaagt atgtcgcatc tggatgaagt catcgcgcgc gtggatgccg
    20281 cgattgaaga gagcgtcatt gcccatatga acgaattatt aatcgccctg agcgatgacg
    20341 cggagttaag tcgggaagat cgctacaccc agcagcagcg tttgcgcacc gctattgctc
    20401 atcacggtcg caaacataaa gaagatatgg aagcgcgcca cgaacagtta accaaaggcg
    20461 gtaccatcct ctgattaaaa tgagcgtctg gccaccagcc aggcgccaac cacaagtaaa
    20521 attgcgccac aaattgggcc gatcaacgcc cgaagtggta cgccatggct gcggaagata
    20581 tccagcacca gaccgccaat cagctggctg gcgaccagta ctgcaatagt tgtcgccgct
    20641 cccacattct gatatccgct gatactggcg aagacaaaaa acgatcccag caagccaggt
    20701 ataagtgtcc accagcgcac gctggacacc agttcgccaa accccgccat gccttgttta
    20761 aaccataaaa tagaaacaaa gagcacaatc cccaccagtg agttcagcaa catggcgatg
    20821 agaatggtag aggaggtctg ggtgatgcgc accatcagcg tgttctggac taccagacca
    20881 atcccggctg caatcaaaaa ggcaagggta agtgactgat tcatccaatc gcgtccggtt
    20941 cagtgcgctc gtcgagttgc aactgcataa atgtcagatc cagccagcgc ccaaatttag
    21001 tgcctacctg cggcatttgc gcggtgacga caaatcccag cgactggtgc agatgcagcg
    21061 aggcctgatt ttgcgattcg atcccggcga ccatgacatg cttcccgcaa tcccgcgctt
    21121 catcaattaa tcggcttaac aatttacgcc ccagaccttt cccctgatga tcgggatgga
    21181 cataaaccga atgttccacg gtatggcgaa aaccatcaaa actacgccag tcgccaaacg
    21241 aggcatatcc cgtcactacg ccatcttctt cactcaccag caccggataa cctgctatgg
    21301 tccgcgcttc aaaccaggca atgcggttat ccgcatccac cgtttggtca ttccagatag
    21361 ccgccgtata caacacggcg tggttataaa tttccgcaat tgcagcacag tcggctttgc
    21421 gggcaaaacg gatggacatg tctggcctcg ataaaataat gatgatgatg ttatcaatac
    21481 tatcgaaaac agcccgaagc aacggattcc tctatgaaat tattgcgtgt tgcagagggg
    21541 atgtgacggc tgcaaacaaa accaatcctg tgagcctgct actattgatg gattcatgcc
    21601 aaaagcacag gagagcatta tggggcaaca aacgcagcgt aaccgatgtt gggttctgtc
    21661 ctcgcgtccg catggcgcgc cggttcctta cgtctggttt cggtaaatgc gctctttgtc
    21721 gcaccagcgc aagcatttac agttccggaa gtgggaaaac gtcaattaaa tgaaatcaat
    21781 cggatccgct acgaactgga gttaatggca gttgctctgg ctgtcgaaaa cctcactatg
    21841 caaaaaaacg ttgctatgtt gtgccagcaa tacagccgat aaataaaata cgcccggcaa
    21901 ataccgggct taaagataat tactcaaatc gccacgcaat atttatgccg acaccataat
    21961 ttcgtccggg cgcaggttcg taatatcgcc cgtttgactc attgacaatg acagaaccaa
    22021 cgtattcttt atcgaataaa ttatcgacac gaccaaataa atccacggtt aaattgtggt
    22081 aattgtattt atacccggtg aataagccga cgagtgtata agaaggcgct ttggccgtat
    22141 tttcatcatc tgccataata tcgcccatat aacgcgcttc cgtgcctgca taccaaccat
    22201 cttccggtat ataacctatc gacgcaaagc ccatattacg ggcgatccct ggcatccggt
    22261 taccgttaca atcctgttca ttgcagacat tgctgcgata ggtcgcatca agccaggtcc
    22321 aggacgcttt tacgcgaaaa tctcccgcga aacgttgatc ccatgccagt tcagcgcctt
    22381 gacgacgggt ctttccggca tttttgtaag tcgtacgccc accgctactg ctatcgacaa
    22441 caatttcatc atcggtgtcg gtctggaaca atgcgagact aagcagtcca tcaccaatac
    22501 gcgttttact gccgatctca attgtatcgt tggtggaggg ttttaaacca aagttcatac
    22561 cgctttgtcc atcagcgcga taagacagtt cattaatcgt cggcgtttca aaaccgcgcc
    22621 cggctgccag atagacattc caggcatcgg tcattgcata ttttaacgat ccggcaggta
    22681 gccatttgtg ataactggca tcaccgctgt catcgccgtt acccggagta acgtaatgat
    22741 cgttggaatc aaaccacacg gagctgtagc gcacgccagc atccagcgaa agtttgtcgg
    22801 tcagttgcca ctgggtttgt aaatagggat cgacgttcca catcaaattg cgttcgtcgc
    22861 ggcgcaactc acctttttgc ccgtattccg gcacgccgcg attcaggcgg aagttattgt
    22921 aacccttgcg gttttcactc atgttttcgt agttcaggcc ggttgtgaac gtgactggaa
    22981 cgcccagttc accacggtgt gtccagcggc tgtctatccc ctggtaatgg cgttgcaggg
    23041 taatcacgcc gcccgcatgt gacgggttaa gttgcggggc catggggatt gactggtact
    23101 gggtcgtttc tcgctctccg gcatacatca tcacactcat atcatcttgt gcgctcaggc
    23161 tacgctcata gcgcaaccca gcctgagttt gcttgatggt ttttcgcatg tcgtactgtt
    23221 ctgcacgagg cgcttgttgt ggattcgcct tccattctgc ttcggttagc ccacctgggt
    23281 catctgcttt gatatccaca ctattgaaaa tcaggcttaa tttgctggcg tcatcaatgc
    23341 gtacgcccag tttggcattg gctaaatttt tctgtgcgcc actatggtca cgatagccgt
    23401 gggtcgtaaa acgtgtggtt gcgacggtgt aatcgacatc gccgggttgt gtgccgtcgc
    23461 ccgttgcgcc cgttgctttc agcccatagc gccagctgcc aaaactgccg tagtaactac
    23521 tggcttcaat ggttggtggc tgttgtccgg tctgggtggt gacattcatt acatcgccag
    23581 acgcgttgcc atacagagca gagaaggggc cacgcagcac ttccacattt tgcacactgc
    23641 ttaaatcgat gttagatgtc tgcccttgtc cgtcgggcat ggtagcggga ataccgtcca
    23701 catacaggcg aataccgcga attccgtaag tggagcggga gccaaatccg cgaatcgaca
    23761 gctgtaaatc ttgcgcatag ttctgtcggt tttgtacctg taaaccaggc actccggtca
    23821 gtgattcaga caagttaatg cgcggtgttg ccaggcgcat ctcctcgcca tccaccacgc
    23881 tcacggctgc tggggtatcc agttctgaaa ccacctgcgg tgcggcactg acaatcatgg
    23941 tctgttcatc agcggcaaaa acaacggggg aaaggacaag cagtgcgggc aaaacggtct
    24001 gtcggacgga aaaaatcttc atgaaaaaag ccaggttaag aatgggaaaa caccgtcatg
    24061 gtaataaaat tgtaaattta tggaaaacaa aacggcacaa tacgttaagt aattgagaaa
    24121 attgtagtca taacggtaag aaatgctcca catttgagaa aataatgatt accattccca
    24181 tttataacaa gggcgtaacg atgattacgc ttagcgaagc attgtgaagc agcaaaaata
    24241 tcggttcatc aaagggagtc gtcatgcatt tacgtcatct gttttcatcg cgcctgcgtg
    24301 gttcattact gttaggttca ttgctcgttg cttcatcatt cagtacgcag gccgcagaag
    24361 aaatgctgcg taaagcggta ggtaaaggtg cctacgaaat ggcttatagc cagcaagaaa
    24421 acgcgctgtg gattgccact tcgcaaagcc gcaaactgga taaaggcggc gtggtttatc
    24481 gtcttgatcc ggttactctg gaagtgacgc aggcgatcca taacgatctc aagccgtttg
    24541 gtgccaccat caataacacg actcagacgt tgtggtttgg taacaccgta aatagtgcgg
    24601 tcacggcgat agatgccaaa acgggcgaag tgaaaggacg tctggtgctg gatgatcgta
    24661 agcgcacgga agaggtacgc ccgttgcaac cacgtgagct ggtagctgac gataccacga
    24721 acaccgttta catcagtggt attggtaaag agagcgtgat ttgggtcgtt gatggcgaga
    24781 atatcaaact gaaaaccgcc atccagaaca ccggtaaaat gagtaccggt ctggcgctgg
    24841 atagcaaagg caaacgtctt tacaccacta acgctgacgg cgaattgatt accatcgaca
    24901 ccaccgacaa taaaatcctc agccgtaaaa agctgctgga tgacggcaaa gagcacttct
    24961 ttatcaacat cagccttgat accaccaatc agcgtgcatt tatcaccgat tctaaagcgg
    25021 cagaagtgtt agtggtcgat acccgtaatg gcaatattct ggcgaaggtt gcggcaccgg
    25081 aatcactggc tgtgctgttt aacccagcgc gtaatgaagc ctacgtgacg catcgtcagg
    25141 caggtaaagt cagtgtgatt gacgcgaaaa gctataaagt ggtgaaaacg ttcgatacgc
    25201 cgactcatcc gaacagtctg gcgctgtctg ccgatggcaa aacgctgtat gtcagtgtga
    25261 aacaaaaatc cactaaacag caggaagcta cccagccgga cgatgtgatt cgtattgcgc
    25321 tgtaatagat atggcgaggg tgaaaacgga aagaattgcc tgacttttat cacgccgcat
    25381 ccggcacctt cgtcagtagt ctgaatttgc ccggcacgta gccgggcaat tttgcaatta
    25441 actcgccgtt tcaggcttaa acacaatgtc ctgtttttct tcatcttcct cgacgactgg
    25501 cgcagtgctg tgaatttcag caacgcgttt gcgcacacca aaccagccta taaccagcag
    25561 aataccgata atcggcagcg ccgcgatggt ataagtcccg ttcgggtaat cgaacgccat
    25621 cagcaccagg acactcagta aaaacagtaa tgtcagccag gaagtgaagg gcgcgccagg
    25681 cagtttaaaa ctgacatccg ctgctttgcc ttctttaatc gctttacgca ggcgcatctg
    25741 gcaaacgatg ataaacgccc atgaagcgat gattcccagc gacgcgaagt tcaacacaat
    25801 ctcaaatacg cgtgacggca ccagatagtt gaggaatacg ccgacgacat acacaaccag
    25861 tgtcgccaga atcccggcat acggcacatg ctgacgactc attttcgcca taaaactcgg
    25921 tgcggaaccg cccatcgcca tcgagcgcag aatacgcccg gtgcagtaca gacctgaatt
    25981 caggctggag agggcagcgg tcagcaccac aatgttcata atgctgccta tatatggcac
    26041 acccagttta gagaaaaacg tcacgaacgg actttgcccc gcctgatatg cgctccacgg
    26101 caataacata accagcaaca ccacggagcc gacgtaaaac agaccaatac gccaaatcac
    26161 actgttaatc gctttaggta ccatggtctg cggatcttta cattctccgg ccgctgtacc
    26221 caccatttca atggaggcaa aagcaaacac tacgccctga atcaacacca gcgcaggcag
    26281 caaaccgtgg gggaagaagc cgccattatc ggtgattaaa tgaaagccag tggtgttgcc
    26341 atccagcggc tgaccgctac cgaggaacac tgtacccacg accaaaaagg ttacaatggc
    26401 gagcacttta ataagcgcaa accagaactc catctccgca aaccatttca caccgatcat
    26461 attcatggtg ccaacgatgg tcagtgcagc gagcgcaaag acccactgcg gcacgccgcc
    26521 aaacgcgccc cagtaatgca tatacagagc gacggcggta atatcaacaa tccccgtcat
    26581 cgcccagttg atgaagtaca tccagccagc aacataagcg gccttctcac ccaaaaactc
    26641 acgggcataa gagacaaaac tgccactgga agggcggtgt aacaccagct cacccaatgc
    26701 acgcaaaata aaaaacgaaa acaggccgca gatcaaatag accagtgcca gtgcgggccc
    26761 cgccatttgc agtcgcgccc ccgcgcctaa aaacaaaccc gtaccaatcg cgccgccaat
    26821 ggcgatcatc tgtacctggc gattgcccat cgctttgtga tacccctctt cgtgggcatt
    26881 aagccagcgg cgtttcgcgg cgtgttgatc tgaagtgtcg gtgttgtgtt tactcattgc
    26941 tctccctgat tgctttaatg aaaaagtcat ataagttgcc atgaacaatg tttattcatc
    27001 ctggcgatga caagattcac cattattctt tatgggtata actgtcggcg cagaatccta
    27061 ccttttatca tgtaacgatg caaaataatc ccgctataaa tatgtgggga taaagtcggc
    27121 tggcgacaaa atagcaacct gatgaaatta tgacggaaac aaccactatt ggtttgatga
    27181 tacctgactg cagtcctact cttgcgccac tcgttcgtcg ctctgaacgc cagtggttgc
    27241 agtggacact ggcgtggttg gctcaatgaa tagtgagggg ctagcacatg atgggtttca
    27301 ggggtaatac gccaaatacc aatacaattg ctgattaaat gagccaattg ccgacattac
    27361 agaaaaggct aaaaaatgta agaggcatgt agggtgataa acgcgctatt gcccaacagg
    27421 agtttctgga attagtcctg ttccatgggc attacaactc ttaaaacatt acttcaatat
    27481 aattaatgtg tatatcattc tcaatattaa tttatgaagt gaatttaata tattccgaat
    27541 tcttaattta cattactaat gta
//
LOCUS       DAHHTG010000059        27458 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.059, whole genome shotgun sequence.
ACCESSION   DAHHTG010000059 DAHHTG010000000
VERSION     DAHHTG010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 27458)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27458)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27458
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.059"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            complement(43..1326)
                     /locus_tag="NBI74_002438"
     CDS             complement(43..1326)
                     /locus_tag="NBI74_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaH/YhbH family protein"
                     /protein_id="HCD1932760.1"
                     /translation="MTWFIDRRLNGKNKSMVNRQRFLRRYKAQIKQSISEAINKRSVT
                     DVDSGESVSIPTEDISEPMFHQGRGGLRHRVHPGNDHFVQNDRIERPQGGGGGSGSGQ
                     GQASQDGEGQDEFVFQISKDEYLDLLFEDLALPNLKQNQQRQLTEYKTHRAGYTANGV
                     PANISVVRSLQNSLARRTAMTAGKRRELHALEENLAIISNSEPAQLLEEERLRKEIAE
                     LRAKIERVPFIDTFDLRYKNYEKRPDPSSQAVMFCLMDVSGSMDQSTKDMAKRFYILL
                     YLFLSRTYKNVEVVYIRHHTQAKEVDEHEFFYSQETGGTIVSSALKLMDEVVKERYNP
                     AQWNIYAAQASDGDNWADDSPLCHEILAKKILPVVRYYSYIEITRRAHQTLWREYEHL
                     QSTFDNFAMQHIRDQDDIYPVFRELFHKQNATAKD"
     gene            complement(1439..3373)
                     /gene="yeaG"
                     /locus_tag="NBI74_002439"
     CDS             complement(1439..3373)
                     /gene="yeaG"
                     /locus_tag="NBI74_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YeaG"
                     /protein_id="HCD1932761.1"
                     /translation="MNIFDHYRQRYEAAKDEEFTLQEFLTTCRQDRSAYANAAERLLM
                     AIGEPVMVDTAQEPRLSRLFSNRVIARYPAFEEFYGMEDAIEQIVSYLKHAAQGLEEK
                     KQILYLLGPVGGGKSSLAERLKSLMQLVPIYVLSANGERSPVNDHPFCLFNPQEDAQI
                     LEKEYGIPRRYLGTIMSPWAAKRLHEFGGDITKFRVVKVWPSILQQIAIAKTEPGDEN
                     NQDISALVGKVDIRKLEHYAQNDPDAYGYSGALCRANQGIMEFVEMFKAPIKVLHPLL
                     TATQEGNYNGTEGISALPFNGIILAHSNESEWVTFRNNKNNEAFLDRVYIVKVPYCLR
                     ISEEIKIYEKLLNHSELTHAPCAPGTLETLSRFSILSRLKEPENSSIYSKMRVYDGES
                     LKDTDPKAKSYQEYRDYAGVDEGMNGLSTRFAFKILSRVFNFDHVEVAANPVHLFYVL
                     EQQIEREQFPQEQAERYLEFLKGYLIPKYAEFIGKEIQTAYLESYSEYGQNIFDRYVT
                     YADFWIQDQEYRDPDTGQLFDRESLNAELEKIEKPAGISNPKDFRNEIVNFVLRARAN
                     NSGRNPNWTSYEKLRTVIEKKMFSNTEELLPVISFNAKTSTDEQKKHDDFVDRMMEKG
                     YTRKQVRLLCEWYLRVRKSS"
     gene            3809..4555
                     /gene="mipA"
                     /locus_tag="NBI74_002440"
     CDS             3809..4555
                     /gene="mipA"
                     /locus_tag="NBI74_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="scaffolding protein MipA"
                     /protein_id="HCD1932762.1"
                     /translation="MTKLKLLALGVLIATSAGVAHAEGKFSLGAGVGVVEHPYKDYDT
                     DVYPVPVINYEGDNFWFRGLGGGYYLWNDATDKLSITAYWSPLYFKAKDSGDHQMRHL
                     DDRKSTMMAGLSYAHFTQYGYLRTTLAGDTLDNSNGIVWDMAWLYRYTNGGLTVTPGI
                     GVQWNSENQNEYYYGVSRKESARSGLRGYNPNDSWSPYLELSASYNFLGDWSVYGTAR
                     YTRLSDEVTDSPMVDKSWTGLISTGITYKF"
     gene            4645..5499
                     /gene="yeaE"
                     /locus_tag="NBI74_002441"
     CDS             4645..5499
                     /gene="yeaE"
                     /locus_tag="NBI74_002441"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal reductase YeaE"
                     /protein_id="HCD1932763.1"
                     /translation="MQQKMIQFSGDVSLPAIGQGTWYMGEDASQRKTEVAALRAGIEL
                     GLTLIDTAEMYADGGAEKVVGEALIGLRENVFLVSKVYPWNAGGQKAINACEASLRRL
                     NTDYLDLYLLHWSGSFALEETVAAMEKLIAQGKIRRWGVSNLDYADMQELWQLPGGNQ
                     CATNQVLYHLGSRGIEYDLLPWCQKQQMPVMAYSPLAQAGRLRNGLLKNAVVNEIAHA
                     HNISAAQVLLAWVISHQGVMAIPKAATVAHVQQNAAVLEVELSSAELVMLDKAYPAPK
                     GKTALDMV"
     gene            complement(5550..6434)
                     /locus_tag="NBI74_002442"
     CDS             complement(5550..6434)
                     /locus_tag="NBI74_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-hexose-6-phosphate mutarotase"
                     /protein_id="HCD1932764.1"
                     /translation="MIKKIFALPVIEQISPVLSRRKLDELDLIVVDHPQVKASFALQG
                     AHLLSWKPAGEEEVLWLSNNTPFKNGVAIRGGIPVCWPWFGPAAQQGLPAHGFARNLP
                     WTLKSHREDADGVALTFELTQSEETKKFWPHDFTLLAHFHVGKTCEIDLEAHGEFETT
                     SALHTYFNVGDIAKVSVSGLGDRFIDKVNDAKEDVLTDGIQTFPDRTDRVYLNPQDCS
                     VINDEALNRIIAVGHQHHLNVVGWNPGPALSVSMGDMPDDGYKTFVCVETAYASETQK
                     VTKEKPAHLAQSIRVAKR"
     gene            complement(6518..7513)
                     /gene="gapA"
                     /locus_tag="NBI74_002443"
     CDS             complement(6518..7513)
                     /gene="gapA"
                     /locus_tag="NBI74_002443"
                     /EC_number="1.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006322611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HCD1932765.1"
                     /translation="MTIKVGINGFGRIGRIVFRAAQKRSDIEIVAINDLLDADYMAYM
                     LKYDSTHGRFDGTVEVKDGHLIVNGKKIRVTAERDPANLKWDEVGVDVVAEATGLFLT
                     DETARKHITAGAKKVVMTGPSKDNTPMFVKGANFDKYAGQDIVSNASCTTNCLAPLAK
                     VINDNFGIIEGLMTTVHATTATQKTVDGPSHKDWRGGRGASQNIIPSSTGAAKAVGKV
                     LPELNGKLTGMAFRVPTPNVSVVDLTVRLEKAATYEQIKAAVKAAAEGEMKGVLGYTE
                     DDVVSTDFNGEVCTSVFDAKAGIALNDNFVKLVSWYDNETGYSNKVLDLIAHISK"
     gene            7855..8268
                     /gene="msrB"
                     /locus_tag="NBI74_002444"
     CDS             7855..8268
                     /gene="msrB"
                     /locus_tag="NBI74_002444"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HCD1932766.1"
                     /translation="MANKPSAEELKKNLSEMQFYVTQNHGTEPPFTGRLLHNKRDGVY
                     HCLICDAPLFHSQTKYDSGCGWPSFYEPVSEESIRYIKDLSHGMQRIEIRCGNCDAHL
                     GHVFPDGPQPTGERYCVNSASLRFTDGENGEEING"
     gene            8310..8582
                     /locus_tag="NBI74_002445"
     CDS             8310..8582
                     /locus_tag="NBI74_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaC family protein"
                     /protein_id="HCD1932767.1"
                     /translation="MNLDDIINSMTPEVYQRLSTAVELGKWPDGVTLTEEQKENCLQL
                     VMLWQARHNTEAQHMTIDTNGQMVMKSKQQLKEDFGISAKPIAMFK"
     gene            8952..10028
                     /locus_tag="NBI74_002446"
     CDS             8952..10028
                     /locus_tag="NBI74_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HCD1932768.1"
                     /translation="MKALARFGKAFGGYKMIDVPQPICGPEDVVIEIKAAAICGADMK
                     HYNVDSGSDEFNSIRGHEFAGCIAQVGEKVKDWKVGQRVVSDNSGHVCGVCPACEQGD
                     FLCCTEKVNLGLDNNTWGGGFSKYCLVPGEILKIHRHALWEIPDGVDYEDAAVLDPIC
                     NAYKSIAQQSKFLPGQDVVVIGTGPLGLFSVQMARIMGAVNIVVVGLQEDVTVRFPVA
                     KELGATAVVNGSTEDVVARCQQICGKDNLGLVIECSGANIALKQAIEMLRPNGEVVRV
                     GMGFKPLDFSINDITAWNKSIIGHMAYDSTSWRNAIRLLASGAIKVKPMITHRIGLSQ
                     WREGFDAMVDKTAIKVIMTYDFDE"
     gene            10055..11434
                     /locus_tag="NBI74_002447"
     CDS             10055..11434
                     /locus_tag="NBI74_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCD1932769.1"
                     /translation="MEQITKPNCGARLDRLPDCRWHSSMFAIVAFGLLVCWSNAVGGL
                     ILAQLKALGWTDNSTTATFSAITTAGMFLGALVGGIIGDKTGRRNAFILYEAIHIASM
                     VVGAFSPNMDFLIACRFVMGVGLGALLVTLFAGFTEYMPGRNRGTWSSRVSFIGNWSY
                     PLCSLIAMGLTPLISAEWNWRVQLLIPAILSLIATALAWRYFPESPRWLESRGRYQEA
                     EKVMRSIEEGVIRQTGKPLPPVVIADDGKAPQAVPYSALLTGVLLKRVILGSCVLIAM
                     NVVQYTLINWLPTIFMTQGINLKDSIVLNTMSMFGAPFGIFIAMLVMDKIPRKTMGVG
                     LLILIAVLGYIYSLQTSMLLITLIGFFLITFVYMYVCYASAVYVPEIWPTEAKLRGSG
                     LANAVGRISGIAAPYAVAVLLSSYGVTGVFILLGAVSIIVAIAIATIGIETKGVSVES
                     LSIDAVANK"
     gene            11451..12494
                     /locus_tag="NBI74_002448"
     CDS             11451..12494
                     /locus_tag="NBI74_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HCD1932770.1"
                     /translation="MKNSKAMLQVPGTMKIISAEIPVPKEDEVLIKVEYVGICGSDVH
                     GFESGPFIPPKDPNQEIGLGHECAGTVVAVGSRVRKFKPGDRVNIEPGVPCGHCRYCL
                     EGKYNICPDVDFMATQPNYRGALTHYLCHPESFTYKLPDNMDTMEGALVEPAAVGMHA
                     AMLADVKPGKKIIILGAGCIGLMTLQACKCLGATEIAVVDVLEKRLTMAEQLGATVVI
                     NGAKEDTIARCQQFTEDMGADIVFETAGSAVTVKQAPYLVMRGGKIMIVGTVPGDSAI
                     NFLKINREVTIQTVFRYANRYPVTIEAISSGRFDVKSMVTHIYDYRDVQQAFEESVNN
                     KRDIIKGVIKISD"
     gene            12515..13351
                     /locus_tag="NBI74_002449"
     CDS             12515..13351
                     /locus_tag="NBI74_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HCD1932771.1"
                     /translation="MLADIRYWENDATNKHYAIAHFNVWNAEMLMGVIDAAEEAKSPV
                     IISFGSGFVGNTSFEDFSHMMVSMAQKATVPVITHWDHGRSMEIIHNAWTHGMNSLMR
                     DASAFDFEENIRLTKEAVDFFHPLGIPVEAELGHVGNETVYEEALAGYHYTDPDQAAE
                     FVERTGCDSLAVAIGNQHGVYTSEPQLNFEVVKRVRDAVSVPLVLHGASGISDADIKT
                     AISLGIAKINIHTELCQAAMVAVKENQDQPFLHLEREVRKAVKERALDKIKLFGSDGK
                     AE"
     gene            13356..14303
                     /locus_tag="NBI74_002450"
     CDS             13356..14303
                     /locus_tag="NBI74_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416286.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HCD1932772.1"
                     /translation="MDNLDVICIGAAIVDIPLQPVSKNIFDVDSYPLERIAMTTGGDA
                     INEATIISRLGHRTALMSRIGKDAAGQFILDHCRKENIDIQSLKQDVSIDTSINVGLV
                     TEDGERTFVTNRNGSLWKLNIDDVDFARFSQAKLLSLASIFNSPLLDGRALTEIFTQA
                     KARQMIICADMIKPRLNETLNDICEALSYVDYLFPNFAEAKLLTGKETLDEIADCFLA
                     CGVKTVVIKTGKDGCFIKRGDMTMKVPAVAGITAIDTIGAGDNFASGFIAALLEGKNL
                     RECARFANATAAISVLSIGATTGVKNRKLVEQLLEEYEG"
     gene            14313..15293
                     /gene="ydjG"
                     /locus_tag="NBI74_002451"
     CDS             14313..15293
                     /gene="ydjG"
                     /locus_tag="NBI74_002451"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent methylglyoxal reductase"
                     /protein_id="HCD1932773.1"
                     /translation="MKKIPLGTADITLSRMGLGTWAIGGGPAWNGDLDRQICIDTILE
                     AHRCGINLIDTAPGYNFGNSEVIVGQALKKLPREQVVVETKCGIVWERKGSLFNKVGD
                     RQLYKNLSPKSIREEVEASLQRLGIDYIDIYMTHWQSVPPFFTPIAETVAVLNALKAE
                     GKIRAIGAANVDADHIREYLQHGELDIIQAKYSILDRAMENELLPLCRDNGIVVQVYS
                     PLEQGLLTGTITRDYVPGGARANKVWFQRENMLKVIDMLEQWQPLCARYQCTIPTLAL
                     AWILKQSDLISILSGATAPEQVRENVAALNINLSDADATLMREMAEALER"
     gene            15430..16188
                     /locus_tag="NBI74_002452"
     CDS             15430..16188
                     /locus_tag="NBI74_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCD1932774.1"
                     /translation="MAAKDRIQAIKQMVANDKKVTVSNLSGIFQVTEETIRRDLEKLE
                     DEGFLTRTYGGAVLNTAMLTENIHFYKRASSFYEEKQLIARKALPFIDNKTTMAADSS
                     STVMELLKLLQDRSDLTLLTNSAEAIHVLAQSEIKVVSTGGELNKNTLSLQGRITKEI
                     ISRYHVDIMIMSCKGLDINSGALDSNEAEAEIKKTMIRQATEVALLVDHSKFDRKAFV
                     QLADFSHINYIITDKSPGAEWIAFCKDNNIQLVW"
     gene            16306..17664
                     /locus_tag="NBI74_002453"
     CDS             16306..17664
                     /locus_tag="NBI74_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCD1932775.1"
                     /translation="MEQYDQIGARLDRLPLARFHYRIFGIISFSLLLTGFLSYSGNVV
                     LAKLVSNGWSNNFLNAAFTSALMFGYFIGSLTGGFIGDYFGRRRAFRINLLIVGIAAT
                     GAAFVPDMYWLIFFRFLMGTGMGALIMVGYASFTEFIPATVRGKWSARLSFVGNWSPM
                     LSAAIGVVVIAFFSWRIMFLLGGIGILLAWFLSGKYFIESPRWLAGKGRIAGAESQLR
                     EVEQQIEREKSIRLPPLTLNQSNSKVKVIKGTFWLLFKGEMLRRTLVAITVLIAMNIS
                     LYTITVWMPTIFVNSGIDVDKSILMTAVIMIGAPVGIFIAALIIDHFPRRLFGSALLI
                     IIAVLGYIYSIQTTEWAILIYGLVMIFFLYMYVCFASAVYIPELWPTHLRLRGSGFVN
                     AVGRIVAVFTPYGVAALLTHYGSITVFMVLGVMLVLCALVLSIFGIETRKVSLEEISE
                     VN"
     gene            complement(17757..18398)
                     /gene="pncA"
                     /locus_tag="NBI74_002454"
     CDS             complement(17757..18398)
                     /gene="pncA"
                     /locus_tag="NBI74_002454"
                     /EC_number="3.5.1.-"
                     /EC_number="3.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001587135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nicotinamidase/pyrazinamidase"
                     /protein_id="HCD1932776.1"
                     /translation="MPPRALLLVDLQNDFCAGGALAVPEGDSTVDVANRLIDWCQSRG
                     EAVIASQDWHPANHGSFASQHGVEPYTPGQLDGLPQTFWPDHCVQNSEGAQLHPLLKQ
                     KAIAAVFHKGENPLVDSYSAFFDNGRRQKTALDDWLRAHVINELIVMGLATDYCVKFT
                     VLDALQLGYKVNVITDGCRGVNIQPQDSAHAFMEMSAAGATLYTLADWEETQE"
     gene            complement(18409..19425)
                     /gene="ansA"
                     /locus_tag="NBI74_002455"
     CDS             complement(18409..19425)
                     /gene="ansA"
                     /locus_tag="NBI74_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HCD1932777.1"
                     /translation="MQKKSIYVAYTGGTIGMQRSEQGYIPVSGHLQRQLALMPEFHRP
                     EMPDFTIHEYTPLMDSSDMTPEDWQHIAEDIKAHYDDYDGFVILHGTDTMAYTASALS
                     FMLENLSKPVIVTGSQIPLAELRSDGQINLLNALYVAANYPINEVTLFFNNRLYRGNR
                     TTKAHADGFDAFASPNLPPLLEAGIHIRRLNTPPAPHGEGELIVHPITPQPIGVVTIY
                     PGISADVVRNFLRQPVKALILRSYGVGNAPQNKAFLQELQEASDRGIVVVNLTQCMSG
                     KVNMGGYATGNALAHAGVIGGADMTVEATLTKLHYLLSQELDTETIRKAMSQNLRGEL
                     TPDD"
     gene            complement(19557..20267)
                     /locus_tag="NBI74_002456"
     CDS             complement(19557..20267)
                     /locus_tag="NBI74_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310500.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HCD1932778.1"
                     /translation="MNKAPSLIAAIVLGLGISACGYFVGDGVKHLKTNNRYVNVRGLS
                     EKEVRADTAELTIAINFKGNIPGELFPKLEDAQKKIVAELNAQGINEKEITLGQWTSK
                     RTDSFYLKDDPTMPRYNADGSVTIKTHNVAAVEKVVAKLNELQVATEGAIAESKVAYR
                     FNGIGALRAEMIAAATKDARNAALQFATDSGSQVGSISDASQGVFQIFASGSDEDDPT
                     AINKTVRVVTTVTYALQD"
     gene            complement(20383..22239)
                     /gene="sppA"
                     /locus_tag="NBI74_002457"
     CDS             complement(20383..22239)
                     /gene="sppA"
                     /locus_tag="NBI74_002457"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HCD1932779.1"
                     /translation="MRTLWRFIAGFFKWTWRLLNFVREMVLNLFFIFLVLVGVGIWMQ
                     VSGGDSKETASRGALLLDISGVIVDKPDSSQRFSKLSRQLLGASSDRLQENSLFDIVN
                     TIRQAKDDRNITGIVMDLKNFAGGDQPSMQYIGKALKEFRDSGKPVYAVGENYSQGQY
                     YLASFANKIWLSPQGVVDLHGFATNGLYYKSLLDKLKVSTHVFRVGTYKSAVEPFIRD
                     DMSPAAREADSRWIGELWQNYLNTVAANRQIPAQQVFPGAQGLLEGLTKTGGDTAKYA
                     LENKLVDALASSAEIEKTLTKEFGWSKTDKNYRAISYYDYALKTPADTGDSIGVVFAN
                     GAIMDGEETQGNVGGDTTAAQIRDARLDPKVKAIVLRVNSPGGSVTASEVIRAELAAA
                     RAAGKPVVVSMGGMAASGGYWISTPANYIVANPSTLTGSIGIFGVITTVENSLDSIGV
                     HTDGVSTSPLADVSITRALPPEVQQMMQLSIENGYKRFITLVADARHSTPEQIDKIAQ
                     GHVWTGQDAKANGLVDSLGDFDDAVAKAAELAKVKQWHLEYYVNEPTFFDKVMDNMSG
                     SVRAMLPDAFQAMLPAPLASVASTVKSESDKLAAFNDPQNRYAFCLTCANVR"
     gene            22400..22951
                     /locus_tag="NBI74_002458"
     CDS             22400..22951
                     /locus_tag="NBI74_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H nitroreductase"
                     /protein_id="HCD1932780.1"
                     /translation="MDALELLINRRSASRLAEPAPTGEQLQNILRAGMRAPDHKSMQP
                     WHFFVIEGEGRERFSAVLEQGAIAAGSDNKAIDKARNAPFRAPLIITVVAKCEENHKV
                     PRWEQEMSAGCAVMAMQMAAVAQGFGGIWRSGALTESPVVREAFGCREQDKIVGFLYL
                     GTPQLKASTSINVPDPTSFVTYF"
     gene            23068..24111
                     /gene="selD"
                     /locus_tag="NBI74_002459"
     CDS             23068..24111
                     /gene="selD"
                     /locus_tag="NBI74_002459"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HCD1932781.1"
                     /translation="MSENSIRLTQYSHGAGCGCKISPKVLETILHSEQAKFVDPNLLV
                     GNETRDDAAVYDLGNGTSVISTTDFFMPIVDNPFDFGRIAATNAISDIFAMGGKPIMA
                     IAILGWPINKLSPEIAREVTEGGRYACRQAGIALAGGHSIDAPEPIFGLAVTGIVPTE
                     RVKKNSTAQAGCKLFLTKPLGIGVLTTAEKKSLLKPEHQGLATEVMCRMNIAGASFAN
                     IEGVKAMTDVTGFGLLGHLSEMCQGAGVQARVDYDAIPKLPGVEEYIKLGAVPGGTER
                     NFASYGHLMGEMPREVRDLLCDPQTSGGLLLAVMPEAENEVKATAAEFGIELTAIGEL
                     VPARGGRAMVEIR"
     gene            24116..26071
                     /gene="topB"
                     /locus_tag="NBI74_002460"
     CDS             24116..26071
                     /gene="topB"
                     /locus_tag="NBI74_002460"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016234421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HCD1932782.1"
                     /translation="MRLFIAEKPSLARAIADVLPKPHRKGDGFIECGNGQVVTWCIGH
                     LLEQAQPDAYDSRYARWNLADLPIVPEKWQLQPRPSVTKQLNVIKRFLHEASEIVHAG
                     DPDREGQLLVDEVLDYLQLAPEKRQQVQRCLINDLNPQAVERAIDRLRSNSEFVPLCV
                     SALARARADWLYGINMTRAYTILGRNAGYQGVLSVGRVQTPVLGLVVRRDEEIENFVA
                     KDFFEVKAHIVTPADERFTAIWQPSEACEPYQDEEGRLLHRPLAEHVVNRISGQPAIV
                     TSYNDKRESESAPLPFSLSALQIEAAKRFGLSAQNVLDICQKLYETYKLITYPRSDCR
                     YLPEEHFAGRHAVMNAISVHAPDLLPQPVVDPDIRNRCWDDKKVDAHHAIIPTARSSA
                     INLTENEAKVYNLIARQYLMQFCPDAVFRKCVIELDIAKGKFVAKARFLAEAGWRTLL
                     GSKERDEENDGTPLPVVAKGDELLCEKGEVVERQTQPPRHFTDATLLSAMTGIARFVQ
                     DKDLKKILRATDGLGTEATRAGIIELLFKRGFLTKKGRYIHSTDAGKALFHSLPEMAT
                     RPDMTAHWESVLTQISEKQCRYQDFMQPLVGTLYQLIDQAKRTSVRQFRGIMAPGGGE
                     GKKKDSPRKRAPKKSPPSEEAGNGVII"
     gene            complement(26148..>27458)
                     /gene="gdhA"
                     /locus_tag="NBI74_002461"
     CDS             complement(26148..>27458)
                     /gene="gdhA"
                     /locus_tag="NBI74_002461"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HCD1932783.1"
                     /translation="NHVQKRDPNQTEFAQAVREVMTTLWPFLEQNPKYRQMSLLERLV
                     EPERVIQFRVVWVDDRNQVQVNRAWRVQFSSAIGPYKGGMRFHPSVNLSILKFLGFEQ
                     TFKNALTTLPMGGGKGGSDFDPKGKSEGEVMRFCQALMTELYRHLGADTDVPAGDIGV
                     GGREVGFMAGMMKKLSNNTACVFTGKGLSFGGSLIRPEATGYGLVYFTEAMLKRHGMG
                     FEGMRVSVSGSGNVAQYAIEKAMEFGARVITASDSSGTVVDESGFTKEKLARLIEIKA
                     SRDGRVADYAKEFGLVYLEGQQPWSVPVDIALPCATQNELDVDAAHQLIANGVKAVAE
                     GANMPTTIEATELFQQAGVLFAPGKAANAGGVATSGLEMAQNAARLGWKAEKVDARLH
                     HIMLDIHHACVEHGGEGEQTNYVQGANIAGFVKVADAMLSQGVI"
BASE COUNT         6874 a         6473 c         7059 g         7052 t
ORIGIN      
        1 gagctaaaaa tcagccaggc gataatgacc cggctgacag ttttagtctt tagctgttgc
       61 attttgttta tgaaacagtt cacggaacac cggataaata tcatcctggt cgcggatatg
      121 ctgcatcgca aagttgtcga aagtagattg cagatgctca tattctcgcc acagtgtctg
      181 atgcgcgcga cgtgtaattt cgatatagct gtaataacga acaacgggta atattttttt
      241 cgccagaatt tcatggcaaa gcggagagtc atcggcccag ttatcgccgt ctgatgcttg
      301 ggctgcgtaa atattccact gtgccgggtt ataacgctct ttcactacct catccatcag
      361 ttttagggcg ctggaaacaa tggtgccgcc agtttcctgc gagtagaaaa actcatgttc
      421 atcgacttct ttcgcctggg tatgatggcg gatgtatacg acttccacgt tcttatacgt
      481 tctgctgagg aacagataca gcagaatata aaaacgttta gccatatctt tagtggactg
      541 atccattgaa ccggaaacgt ccatcaggca aaacatcact gcctggctgg agggatcggg
      601 ccgtttttcg tagttcttgt aacgtaaatc gaaggtgtca atgaaaggga cgcgttcaat
      661 tttggcacgt aattctgcaa tttctttgcg cagacgttcc tcttccagca gttgcgcagg
      721 ttcactgttg ctgatgatgg ccaaattctc ttccagcgca tgaagttccc gccgcttgcc
      781 cgccgtcata gctgtgcgcc gcgccagtga gttctgcaat gaacgtacaa cgctgatatt
      841 ggccggaacg ccgttagcgg tataacccgc ccgatgcgtt ttatattcgg tcagctggcg
      901 ttgttggttt tgtttcagat tcggtaaggc caaatcttca aagagcagat caagatactc
      961 atctttcgaa atctgaaaga caaattcatc ctgaccttca ccatcctggc tggcctggcc
     1021 ctgaccactg ccggaacctc cgccaccacc ctggggacgt tcaattcggt cgttctggac
     1081 gaaatggtca ttgcccggat gcacacggtg gcgcaggccc ccacgcccct gatgaaacat
     1141 cggttcgcta atatcttccg tgggaatgga tacggactcg ccgctgtcga cgtcagtcac
     1201 cgaacgctta ttaatggcct cggagatcga ctgtttaatt tgcgctttat aacggcgtaa
     1261 gaaacgctgg cgattcacca tgcttttatt tttgccgttc agacgccggt caataaacca
     1321 ggtcatatgc cccccgtact acatttgcca acggtatgct acgcttgtgt tgccgcttct
     1381 ctggcccacg gttcgctgtc gttgtaggcc cggtagcatg tgtaccgggc cagggttgtt
     1441 aagacgattt acgtacgcgc aaataccatt cgcacagtaa acgcacctgt ttacgggtgt
     1501 agcctttttc catcatacgg tcgacaaagt cgtcgtgttt cttctgctcg tcggttgacg
     1561 ttttggcgtt aaacgaaata accggcaaca gctcttcggt attggagaac attttcttct
     1621 cgataaccgt gcgcagtttt tcatagctgg tccagttcgg attgcgtccg ctgttattcg
     1681 ctctggctcg cagtacgaag ttaacaatct cgttgcggaa atcttttgga ttactgatcc
     1741 ccgccggttt ctcgattttc tccagctcgg cgttaagaga ctcgcggtca aacagctgcc
     1801 cggtatccgg atcgcgatac tcctgatcct gaatccagaa atccgcgtag gtaacataac
     1861 ggtcgaaaat gttttgccca tattctgaat aggattcaag gtaggccgtc tggatctctt
     1921 tgccgataaa ctcggcatat ttcgggatca gataaccttt caggaactcc agatagcgtt
     1981 cagcctgctc ttgcgggaac tgctcgcgct cgatctgctg ttccaggacg tagaacagat
     2041 ggaccgggtt tgccgccact tctacatgat cgaagttgaa cacgcgggag aggatcttaa
     2101 acgcaaaacg cgtcgacaga ccgttcatcc cttcatcgac accggcgtag tcacgatatt
     2161 cctgatacga cttggctttg ggatcagtgt ctttcagact ttcaccatca taaacgcgca
     2221 tctttgaata aatgctggag ttttctggct ctttcagacg cgaaagaata gaaaaacgtg
     2281 acagcgtttc cagcgtgcca ggggcgcagg gggcgtgagt caattcactg tgattaagca
     2341 atttctcgta gattttgatc tcttcggaaa tgcgcaagca atacggcacc ttcacgatgt
     2401 aaacacggtc gaggaaggct tcgttgtttt tgttattacg gaaagtgacc cattcggatt
     2461 cgttcgagtg ggcgagaata atcccgttga acggcagggc ggagatacct tccgtcccgt
     2521 tgtagttacc ttcctgggtg gcggttaaca agggatgcag cacttttatc ggtgctttaa
     2581 acatctcgac aaattccatg atcccctgat tggcgcggca cagcgcaccg gaatagccgt
     2641 aggcgtccgg gtcattctgc gcgtagtgtt cgagtttacg aatatcgact ttccctacca
     2701 gggcggagat gtcctggttg ttctcatctc cgggttccgt tttggcgatg gcaatttgtt
     2761 gcagaattga cggccagacc ttcactaccc ggaacttagt gatatcgcca ccaaattcat
     2821 gcaggcgctt tgccgcccac ggcgacatga tggtgccgag ataacggcga ggaatgccgt
     2881 actctttttc cagaatctgc gcatcttcct gcggattgaa aagacagaac ggatgatcgt
     2941 tgaccgggct acgctcaccg ttggcgctca atacataaat cggtacgagc tgcattaatg
     3001 atttcagtcg ctcagcaagt gatgatttac ccccacccac aggccccagc agatacagaa
     3061 tttgtttctt ctcttccagc ccctgagccg cgtgtttcag ataagaaaca atctgttcaa
     3121 tcgcgtcttc catgccgtaa aactcttcaa acgccggata acgtgcaatg acccggttag
     3181 aaaagagtcg agaaagtctg ggttcctggg ctgtatcgac catgacaggc tcaccaatag
     3241 ccatcaatag ccgctcagcc gcgttggcat aagcactgcg atcttgccga caagtggtaa
     3301 gaaactcctg cagtgtgaac tcttcgtcct tggcagcttc atatcgctgg cgatagtgat
     3361 cgaatatatt catggtatgc cgtcctttcg ttttttagca caggttaaga gccgttcata
     3421 tgaatagtag aggctcccgg aagaggtaaa ctgaacgacg tctcttgcta aaacacagca
     3481 actctcatgc cattttattg acgatgagaa caacgtgccc ggtgatacac cttcaaaatt
     3541 aagcgtagat ggcaaatgga aaacttgcct gcacggcttt cttaatttca atggcatatc
     3601 aacaactcat ccataaattt tgcataatta atgtaaataa tagggtcgcc ggtaacgcta
     3661 aattcatttg gctgtaagcg tggtgtcatc tgcgtcagga aaattaaaca gttactataa
     3721 aaaatgaaaa cgtaaaaagg ttgggtttcg atgtattgac gggtaaactt tgtcgcccgc
     3781 taaacatttg tttaaggaat gattaattgt gaccaaactc aaacttctgg cacttggagt
     3841 gcttatcgca acgtctgcag gcgtagcgca cgctgaaggt aaattttccc tgggcgcagg
     3901 cgtaggtgtc gttgaacacc catataaaga ttacgatacc gatgtttacc cagtaccggt
     3961 aatcaactat gaaggcgata acttctggtt ccgtggctta ggtggtggtt actacctgtg
     4021 gaatgacgcg acggacaaac tttcaattac cgcttactgg tcgccgcttt acttcaaagc
     4081 taaagacagt ggcgatcacc aaatgcgtca cctcgatgac cgtaagagca ccatgatggc
     4141 tggtctgtct tatgctcact ttacccagta cggttacctg cgtaccaccc tggctggcga
     4201 taccctggat aacagcaacg gcatcgtctg ggatatggcc tggttgtatc gttacaccaa
     4261 cggtggcctg accgtgactc cgggtattgg tgtgcagtgg aacagcgaaa accagaacga
     4321 atactattat ggcgtatcgc gcaaagagtc cgctcgcagc ggtctgcgtg gctataaccc
     4381 gaacgacagc tggagccctt acctggagct gagcgccagc tacaacttcc tcggcgactg
     4441 gagtgtttac ggtaccgcgc gctacacccg tctgtctgat gaagttactg acagcccaat
     4501 ggtggataaa tcctggactg gcctgatttc taccgggatc acctacaaat tctgataatg
     4561 catctttcca gggcattttg tgcataaaaa cagggcgtaa tgccctgttt tgcattacgg
     4621 tggtgcaata cctgggagga aacaatgcaa caaaaaatga ttcaatttag tggcgatgtc
     4681 tcactgccag ccatagggca gggaacatgg tatatgggcg aagatgccag tcagcgtaaa
     4741 acagaagttg ctgcactacg cgcgggcatt gaactcggtt taaccctcat tgataccgcc
     4801 gaaatgtatg ccgatggcgg tgccgaaaaa gtggttgggg aagcattaat cggtctgcgt
     4861 gagaacgtct ttctcgtctc taaagtctat ccgtggaatg ctggcgggca aaaagcgata
     4921 aatgcatgcg aagccagttt acgccgtctc aatactgatt atctcgatct ctacttatta
     4981 cactggtctg gcagtttcgc tctcgaagag actgtcgcag cgatggaaaa attgatcgcc
     5041 cagggaaaaa tccgccgctg gggcgtttct aaccttgatt atgccgatat gcaggaactc
     5101 tggcagctgc cggggggaaa tcagtgtgcc actaatcagg tgctttacca tctcggttca
     5161 cgagggattg agtacgatct acttccctgg tgccagaaac agcagatgcc ggtgatggct
     5221 tacagtccat tagcccaggc cgggcggttg cgcaatggac tgttaaaaaa tgcggtagtc
     5281 aacgaaattg cacatgctca caatatcagt gcggcacaag tattgttggc gtgggtaatc
     5341 agccatcagg gtgtgatggc gattccaaaa gcggcaactg tcgcacatgt ccaacaaaat
     5401 gcggctgtgc ttgaggttga actttcgtca gcggaattag ttatgctgga taaagcgtat
     5461 ccggcaccaa aaggaaaaac tgcgctggat atggtgtgat gttgattcgt gccggatgca
     5521 tcgcacccgg cacacaatat taacgcaaat taacgtttag caacgcgaat ggattgcgcc
     5581 agatgtgcag gtttctcttt ggtgactttt tgcgtttctg aagcgtaagc cgtttctaca
     5641 caaacaaagg tcttgtagcc atcatccggc atatcgccca tgctaactga aagcgccggt
     5701 cctgggttcc agcctacaac gttcagatga tgctggtggc ctacggcgat aatacgattc
     5761 agcgcttcgt cattaattac gctgcaatct tgcggattca gatacacgcg atcggtacgg
     5821 tcagggaagg tctgaatacc gtcggtcagt acatcttctt tcgcgtcatt tactttatca
     5881 atgaagcgat cgcctagtcc actgacgctt accttagcga tatcacccac gttaaagtag
     5941 gtatgcagag cagaggtagt ttcaaattcg ccgtgtgctt caagatcgat ttcacaggtt
     6001 ttacccacgt ggaaatgcgc taacagcgta aagtcgtgcg gccagaattt tttcgtctct
     6061 tcgctttgtg tcaattcaaa agtcagcgct acaccatcag catcttcacg atgtgatttc
     6121 agcgtccacg gcaggttgcg ggcaaaaccg tgcgcaggca gaccttgttg tgcagccgga
     6181 ccaaaccacg gccagcaaac cggtatgcca ccgcgaatgg cgacgccatt tttgaacggt
     6241 gtgttgttac tcaaccacag aacttcttct tcacccgcag gtttccacga gagaaggtgt
     6301 gcgccctgta atgcaaaaga ggcttttacc tggggatgat cgaccacaat gaggtccagt
     6361 tcatccagtt tacgacggga gaggacaggg gagatttgtt cgatgaccgg aagggcaaaa
     6421 attttcttaa tcatgacgca gtcctttaac ttcattttat caggtaaaaa aaagagcgac
     6481 cgaagtcgct ctttttagat cacagtgtca tctcaactta tttggagatg tgagcgatca
     6541 ggtccagaac tttgttggag taaccggttt cgttgtcgta ccaggatacc agtttcacga
     6601 agttgtcgtt cagagcgata cccgctttag catcgaacac ggaagtgcaa acttcgccgt
     6661 tgaaatcggt agatactacg tcatcttcgg tgtagcccag aacgcctttc atttcgcctt
     6721 cagcagcagc tttaacggca gctttgatct gctcgtaagt tgcagctttt tccagacgaa
     6781 cggtcaggtc aactacggat acgttcgggg tcggaacgcg gaacgccata ccagtcagtt
     6841 tgccattcag ttctggcagt actttaccta cagctttagc agcaccggta gaagacggga
     6901 tgatgttctg ggaagcgccg cggccgccgc gccagtcttt gtgagacggg ccatcaacgg
     6961 ttttctgagt agcggtagta gcgtgaacgg tggtcatcag accttcgatg atgccgaagt
     7021 tatcgttgat aactttagcc agcggagcca ggcagttggt ggtgcaggat gcgttggaaa
     7081 cgatgtcctg gccagcatat ttgtcgaagt tagcgccttt aacgaacatc ggagtgttgt
     7141 ctttagacgg accagtcata accactttct tcgcaccagc ggtgatgtgt ttacgagcag
     7201 tttcgtcagt caggaacaga ccagttgctt cagcgacaac gtcaacacca acttcgtccc
     7261 atttcaggtt agccggatca cgttcagcgg taacacggat ttttttaccg ttaacgatca
     7321 gatgaccgtc tttcacttca acggtaccgt cgaaacggcc gtgagtggag tcatatttca
     7381 gcatgtatgc catgtaatca gcgtctaaca ggtcgttgat tgcaacgatc tcgatgtcag
     7441 aacgtttctg agcagcacgg aaaacaatgc gaccgatacg gccaaaaccg ttgataccta
     7501 ctttgatagt catatattcc accagctatt tgttagtgaa taaaaggttg cctgtaaaat
     7561 tacaaaaacc ttacgcagcg tcaagcggaa tcgtgtcaat cattgcgaca aatcaatcct
     7621 gtgcctaagc attacgcgac tgactcgcct cactcttcct ttgggcttga gaccacatgg
     7681 ggtcggcgcc ccgaatttta aagggcaatt acgataaaaa tgtgattttc atcacgattt
     7741 aggtgcagcc atttcgtcga gatcaagttt aggacaaaat tttgccagtt ggtgttaatg
     7801 ttttgttaga atcagtcacg taatgtgagc acgattaaag tgagatgtga gcaaatggct
     7861 aataaacctt cggcagaaga actgaaaaaa aatttgtccg agatgcagtt ctacgtgacg
     7921 cagaatcatg ggacagaacc gccatttacg ggtcgtttac tgcataacaa gcgtgacggc
     7981 gtatatcact gtttgatctg cgatgccccg ctgtttcatt cccaaaccaa gtatgattcc
     8041 ggctgtggct ggcccagttt ctacgaaccg gtgagtgaag aatctattcg ttatatcaaa
     8101 gacttgtcac atggaatgca gcgcatagaa attcgttgcg gtaactgcga tgcccatctg
     8161 ggccatgtct ttcctgacgg gccgcagcca acgggcgaac gttattgtgt taactctgct
     8221 tctttacgct tcactgatgg cgaaaacggc gaagaaatca acggttgaaa aaacgattca
     8281 gcaacttatt ccacaggagc ggaaaaatta tgaatcttga tgacattatc aacagcatga
     8341 cgcctgaggt ataccagcgt ttgtcgaccg ccgttgaact ggggaaatgg cctgatggcg
     8401 ttacgttgac cgaggaacaa aaagagaact gcctgcaact ggtgatgctg tggcaagccc
     8461 gccataatac cgaagcacag catatgacaa ttgacacaaa tggtcagatg gtcatgaaga
     8521 gcaaacagca gttaaaagaa gattttggta tctcagcaaa gcctattgcc atgtttaagt
     8581 aagtggttta tccccttttt gccgcggtta taaaggggat agccaacaat gaccccacga
     8641 ctcatctcaa ttatctcgcc ccgacctggc taagtcccag tccgatctct tgcagtcgcg
     8701 ttcacacctg aatgacttct cctgaactta agtctcaggc ctatttggcc gggaatccct
     8761 ctcgaatttg ctctttttgc gtcccgcctt ccatcaacct gtaacaaccc cagccatttt
     8821 ctgcgtaatt gtgatctaag ttgtaaaacg cagatcatta tctgtgtttt acagatttaa
     8881 tgttgacctc aaaaatcatg tgggtaagta tgatgaccat ctaagcaaaa caaaacacag
     8941 aggatgacgc aatgaaagca ctggctcggt ttggcaaggc ttttggtggc tacaagatga
     9001 ttgatgtccc acaacccatt tgtggcccgg aagatgtcgt gattgaaatt aaagccgcgg
     9061 caatctgcgg cgcagacatg aagcactaca atgtcgatag cggttcggat gagtttaact
     9121 ctatccgcgg ccatgagttc gcaggttgta ttgcgcaggt tggtgaaaaa gtcaaagact
     9181 ggaaagtggg gcaacgcgtc gtgtcggata acagcggtca cgtttgcggc gtttgcccgg
     9241 cctgtgaaca aggtgatttt ctgtgttgta cagaaaaagt aaaccttggt ctggataaca
     9301 atacctgggg cggtggtttt tccaaatatt gtcttgttcc tggtgaaatt ctcaaaattc
     9361 atcgtcatgc gttgtgggaa atccctgatg gtgttgatta tgaggacgca gccgtacttg
     9421 accctatctg taatgcctat aaatccatcg cgcagcaatc gaaattcctt ccaggtcagg
     9481 atgtggtcgt catcggcact ggcccactcg ggctgttctc cgtacaaatg gcgcggatta
     9541 tgggggcggt aaatatcgtc gtcgttggtc tgcaagaaga tgtaacggtc cgcttcccgg
     9601 ttgcaaaaga actgggtgcg acggcagtag taaatggttc taccgaagat gtggtggcac
     9661 gctgccagca aatttgtggc aaagacaatc tggggctggt gattgaatgc tccggtgcca
     9721 atatcgcatt gaaacaagcc atcgaaatgc tccgcccgaa tggggaagta gtacgcgttg
     9781 gaatgggctt caaacctctt gatttctcga ttaatgacat taccgcctgg aacaaaagca
     9841 tcattgggca tatggcctat gactccactt catggcgtaa cgctatcagg ctattagcca
     9901 gcggcgctat caaagtcaaa ccgatgatca cgcatcgtat cggcctgtcg caatggcgcg
     9961 aagggtttga tgcgatggtc gataaaactg caatcaaagt gatcatgact tacgactttg
    10021 atgaataact actctcctga aggaacgtta tcgcatggaa cagataacaa aaccgaattg
    10081 tggtgcacgg ctggatcgct taccggattg ccgctggcat tcgtcgatgt ttgccatcgt
    10141 cgcgtttggc ctgcttgtct gctggagtaa tgccgttggt ggcttgatcc tcgcgcagct
    10201 gaaagcgttg ggctggacag ataattccac cactgccaca ttctcagcaa tcacgaccgc
    10261 cggaatgttt ctcggtgcgc tggtgggcgg catcatcggt gacaaaaccg gtcgcagaaa
    10321 tgcgtttatc ctctatgagg ccattcatat tgcctcgatg gttgtcggtg ctttctcacc
    10381 gaatatggat ttccttattg cctgccgttt tgtgatgggc gttgggctgg gagctttact
    10441 ggttacactg tttgctggtt tcaccgaata tatgcccgga agaaatcgcg gaacgtggtc
    10501 cagtcgggtt tcctttattg gtaactggtc atatccgctc tgttcattga ttgcgatggg
    10561 actcacgccg ctgattagtg cagagtggaa ctggcgggta caactgctta tccctgcaat
    10621 attgtcactt atcgctacgg cgctggcctg gcgctacttt cctgaatccc cgcgctggct
    10681 ggaatcgcgc ggacgttatc aggaagccga gaaagtcatg cggagtatag aagaaggcgt
    10741 catacgccag acgggtaaac ctttgccgcc tgtggttatt gctgatgacg gtaaagcacc
    10801 acaagcggtg ccgtattcag ccttactgac aggagtatta ctgaaacgcg tgatattagg
    10861 ctcttgtgtg ctgattgcca tgaacgttgt gcagtacaca ctaattaact ggttgccaac
    10921 gatattcatg acccagggaa ttaatttaaa agactcgatt gttttaaata ccatgagtat
    10981 gtttggcgcg ccatttggta tttttattgc catgctggtg atggataaaa ttccgcgtaa
    11041 aacaatgggt gtggggctat taatcctgat tgcggtgctc ggatatatct attcactgca
    11101 aaccagtatg ttgctcataa cgctgattgg tttcttcctg attactttcg tctatatgta
    11161 cgtttgctat gcctcggcag tgtatgtccc tgagatctgg ccgacagagg ccaaactccg
    11221 tggctccggt ctggcgaatg cggtagggcg aatcagtggt attgccgcac cttatgccgt
    11281 tgcagtgctg ctcagtagtt atggcgtaac gggagtcttt attcttctgg gggctgtttc
    11341 aattattgtc gcaattgcta tcgccaccat tggaattgaa accaaaggtg tctccgttga
    11401 aagtttaagt attgatgcag tagccaataa ataatactgg tggattcaaa atgaaaaatt
    11461 caaaagcaat gttgcaggtg ccgggcacaa tgaaaattat ttcagcagaa atacctgtac
    11521 ctaaagaaga tgaagttttg attaaagtgg aatatgtcgg tatttgtggt tcagatgtac
    11581 atggttttga atcaggcccg tttattcctc ctaaagaccc aaatcaagaa attggcctgg
    11641 gtcatgaatg cgccgggacg gttgtggctg tgggaagccg cgtgcgcaaa tttaaaccgg
    11701 gggatcgggt aaatatcgaa cctggcgttc cttgcggtca ctgtcgttac tgtctggaag
    11761 gcaaatataa catctgcccg gacgttgatt ttatggcgac acaacccaac taccgcggcg
    11821 cattaacgca ctatctgtgt catccggaga gctttactta caaactgcca gacaatatgg
    11881 acacgatgga aggggcgctg gtggagcctg ccgcagtcgg gatgcatgcc gcgatgctgg
    11941 cagatgttaa accgggtaag aagataatta ttctgggagc gggttgtatt ggtttgatga
    12001 cgttgcaagc gtgcaaatgc ctgggagcaa cggaaattgc cgtcgttgat gtgctggaaa
    12061 aacgtctgac aatggcggaa cagctcggcg cgacagtggt tattaacggc gcaaaagaag
    12121 acactattgc acgctgtcag caatttaccg aagacatggg cgcagatatt gtttttgaaa
    12181 cagcgggttc tgcggtcacc gttaaacagg caccttatct ggtcatgcgc ggcggtaaaa
    12241 ttatgattgt tggtactgta cccggcgatt cggcaatcaa tttcctcaaa atcaatcgcg
    12301 aagtcactat ccagacggta ttccgctatg ccaatcgtta tccggtcacg attgaagcta
    12361 tttcttcagg gcgattcgat gtgaaatcga tggtgacgca tatttacgat tatcgggatg
    12421 tacaacaggc atttgaagag tcagttaaca acaaacgcga cattattaaa ggcgttatta
    12481 aaattagcga ttaattaata cgggagaaca gaacatgctc gcagatatca gatattggga
    12541 aaacgatgcc acaaataaac attatgcaat tgcccatttt aacgtatgga atgcagaaat
    12601 gttgatgggc gttatcgacg cagccgaaga agcgaaatca ccggttatta tttcttttgg
    12661 ttcaggtttt gtcggcaaca cctcatttga agatttctct cacatgatgg tatcaatggc
    12721 acaaaaagca acggtgccgg taataactca ttgggatcat ggtcggagta tggagattat
    12781 tcataacgcc tggactcatg gtatgaattc attaatgcgt gatgcttctg catttgattt
    12841 cgaagaaaat attcgtttaa ccaaagaggc tgttgatttc ttccatccgc tgggtattcc
    12901 ggtagaggcg gaattagggc atgtcggtaa tgaaaccgtt tatgaggaag cgctggcggg
    12961 ctatcactat accgatcctg accaggcggc tgaatttgtt gaacgtacgg gctgtgactc
    13021 attggctgtc gccatcggca accagcatgg ggtttatacg tcagagccac aattgaactt
    13081 tgaggtcgtc aaacgcgtac gcgatgctgt ttctgttccg ctggttttgc atggcgcatc
    13141 ggggatcagt gatgccgaca ttaaaactgc aatttcgttg ggtattgcga aaatcaacat
    13201 tcatacggag ctctgtcagg ccgcaatggt agcagtgaaa gaaaaccagg atcagccttt
    13261 cctgcattta gaacgtgaag tgcgtaaagc tgtaaaagaa cgggcattgg ataaaattaa
    13321 actgttcggt tctgatggca aagcggaata ataatatgga taatctcgac gttatttgta
    13381 taggtgccgc tattgttgat attccattgc aaccggtcag taaaaatatc tttgatgtgg
    13441 attcttatcc tcttgaaaga atcgcaatga ccaccggggg tgatgcaata aatgaagcaa
    13501 caattatttc tcgtctgggc catcgcacag cattaatgag tcgtattggt aaagatgccg
    13561 cagggcaatt tattctcgac cattgccgca aagagaatat tgatattcaa agcctgaagc
    13621 aggacgtcag catagatacc tctattaacg ttggactggt gacggaggat ggcgagcgga
    13681 cctttgtcac aaaccgtaac ggcagtctgt ggaaattgaa tattgacgat gttgattttg
    13741 cgcggttttc tcaggcgaaa ttattatcac tggccagtat tttcaacagt cctctattgg
    13801 atggtagagc gctaacagaa atttttacgc aagctaaagc ccggcagatg attatctgtg
    13861 ccgatatgat caaaccgcgg ttgaatgaaa cgctgaatga tatttgcgaa gcattgagtt
    13921 atgtcgatta tctgtttcct aattttgccg aggcaaaatt actcaccggg aaagagacac
    13981 tggatgaaat tgctgactgc tttcttgcgt gcggcgtaaa aacggtggtg attaaaacgg
    14041 gtaaagacgg ctgctttatc aagcgtggtg acatgacgat gaaggtgccg gcggtcgcag
    14101 gaataaccgc tatcgacacc attggtgcgg gcgataactt tgcttcaggt tttattgcag
    14161 cactgttaga aggcaaaaat ctgcgagaat gcgcacgctt tgccaatgca acggcggcta
    14221 tctcggttct aagcatcggc gccaccaccg gtgtaaaaaa cagaaagctg gtcgaacaat
    14281 tgctggaaga atacgaagga taatgaaggc aaatgaaaaa gataccttta ggcacagcgg
    14341 atataacgct ttcgcgaatg gggttgggga catgggctat tggcggcggt cctgcatgga
    14401 atggcgatct cgatcgacaa atatgcattg atacgattct tgaagcccat cgttgcggca
    14461 ttaacctgat tgatactgcg ccaggatata actttggcaa tagtgaagtt atcgtcggtc
    14521 aggcgttaaa aaaactgccc cgtgaacagg ttgtagtaga aaccaaatgc ggcattgtct
    14581 gggaacgaaa agggagttta ttcaacaaag ttggcgatcg gcagttgtat aaaaaccttt
    14641 ctccgaaatc tatccgcgaa gaggtagaag ccagcttgca acgtctgggt attgattaca
    14701 tcgatatcta catgacgcac tggcagtcgg tgccgccatt ttttacgccg atagctgaaa
    14761 ctgtcgcagt gcttaatgcg ttaaaggccg aagggaaaat tcgcgctata ggcgctgcta
    14821 acgtcgatgc tgaccatatc cgcgagtatc tgcaacatgg tgaactggat attattcagg
    14881 cgaaatacag tatcctcgac cgggcaatgg aaaacgaact gctgccgcta tgtcgtgata
    14941 atggcattgt ggtacaggtc tactctccgc tagagcaggg attgttgacc gggaccatca
    15001 cgcgtgatta cgttccgggc ggcgctcggg caaataaagt ctggttccag cgtgaaaaca
    15061 tgctgaaagt gattgatatg cttgaacagt ggcagccact ctgtgctcgt tatcagtgca
    15121 caattcccac tctggcactg gcgtggatat taaaacagag tgatttaatc tccattctta
    15181 gtggggctac tgcgccggaa caggtacgtg aaaatgtcgc ggcactgaat atcaacttat
    15241 cggatgcaga cgcaacattg atgagggaaa tggcagaggc cctggagcgt taaatactca
    15301 acacgattca cctctgtttc tgtgaaaact gtgatgaaaa tctgtgattc aaacaggtta
    15361 ttttgaaagt aaacatcggt tatgcgataa ttgcgctaat gtgatgtaaa aagatacagg
    15421 ggtgtaatcg tggcggcaaa agacaggatt caggcaatta agcaaatggt tgccaacgat
    15481 aaaaaggtga cagtctcaaa tttgagtggg atttttcagg taaccgaaga aaccattcgc
    15541 cgcgatcttg agaagttgga agatgaaggc tttttgacca gaacctatgg tggtgctgtt
    15601 ttaaatacag cgatgttgac ggagaatatc catttttata agcgcgcttc atcgttttat
    15661 gaagagaagc agcttattgc acgcaaggca ctacccttta tcgacaataa aaccaccatg
    15721 gcagccgatt ccagtagtac cgtaatggaa ttgctcaaat tgttacagga ccgtagtgac
    15781 ctgacgttgc taaccaactc ggcggaagcg attcatgttc tggctcagtc agaaattaaa
    15841 gtcgtttcaa cgggtgggga actaaacaaa aatacacttt cactgcaagg aagaattact
    15901 aaagagatca tcagccgcta ccatgtcgat atcatgataa tgagttgtaa aggtcttgat
    15961 attaacagcg gcgcgctgga ctctaatgaa gcggaagctg aaatcaaaaa gacaatgatc
    16021 cgtcaggcga cagaagttgc gttattggtt gatcactcta agtttgatcg caaagctttt
    16081 gtccagttag ctgactttag tcatattaat tacataataa ctgataaatc accgggtgca
    16141 gagtggatag cattttgcaa agacaataat atccagctgg tgtggtaatt taagcacaca
    16201 ttgtcaatat tatttgttcc tgcgcctgga aataaaatga tgataaagat gcggcaagtg
    16261 aatttgccgc aattgctacc ttttttagca aatccgaggc acccaatgga acaatatgat
    16321 caaattggcg caagactgga ccgcttgcct ttggcccggt ttcattatcg tatatttggt
    16381 ataataagct ttagtctgtt attaacgggg tttttgagtt actccggtaa tgtcgtctta
    16441 gcaaagctgg taagcaatgg atggtcaaat aatttcctca atgccgcctt tacctcggca
    16501 ttaatgtttg gttatttcat cggctctctt accggtgggt ttattggtga ctactttggg
    16561 cggcgcaggg cgtttcgcat aaatcttctc atcgtcggta ttgcggcaac aggggccgct
    16621 tttgtccctg atatgtactg gctcatcttc tttcgcttcc tgatgggaac aggaatgggg
    16681 gcgctgatta tggttggcta tgcctcattt acggagttta tccccgcgac ggtgcgtgga
    16741 aaatggtccg cgcggctctc atttgttgga aactggtcgc ccatgctgtc tgcggcgata
    16801 ggcgtggtgg ttatcgcttt ttttagttgg cggataatgt ttctgctggg cggtattggc
    16861 atactgttag cctggtttct ctcaggtaaa tactttattg agtcgccacg atggttggca
    16921 gggaaagggc gaatcgcagg tgcagaaagc caacttcgtg aagtagagca gcaaattgaa
    16981 agagagaaga gtattcgttt acccccgctt actttgaacc agagcaacag caaggttaag
    17041 gtaatcaagg gtaccttctg gctcctgttt aaaggggaaa tgttacgacg tacattagtc
    17101 gcgattactg ttttaattgc aatgaacatt tcgctttata ccatcaccgt atggatgccg
    17161 accatatttg ttaactccgg cattgatgtc gataaatcaa tattaatgac cgctgttatt
    17221 atgattggcg ctccggtagg gatatttatt gcggcattaa ttattgatca tttccctcgt
    17281 cgattatttg gttccgcctt acttattatt attgccgtgt taggctatat ctattcaatt
    17341 cagacaacag agtgggcgat tttaatctat ggcctggtga tgatcttctt tttatacatg
    17401 tatgtttgct tcgcgtcggc ggtttatatc ccggaacttt ggccaacgca tttacgcctg
    17461 cgtggttcgg gtttcgttaa tgccgtagga cggatcgtcg cagtcttcac gccctatggc
    17521 gttgcggcat tattaacaca ttatgggtcg atcacggtgt ttatggtgct tggtgtcatg
    17581 ttagtgctct gtgcgctggt tctctccatt tttggcatcg aaacgcggaa ggtgtcgctg
    17641 gaagagattt ctgaggtgaa ttaaatcttt cgttgatggc gtcaattacc agatgtatca
    17701 ctgatgtgtt ttatctggcc tacaatgcca gtacagaatt gtaggccagc gtaaaattac
    17761 tcctgcgttt cttcccagtc tgccagcgta tatagcgttg ccccagctgc tgacatttcc
    17821 ataaacgcgt gcgcactgtc ctgtggctgg atatttacgc cacgacaacc atcggtaatc
    17881 acgttcacct tatagcctaa ctgtaaagcg tccagcacgg taaacttaac gcaatagtca
    17941 gtagccagac ccataacgat caattcattg attacatgag cgcgcaacca gtcatcgagc
    18001 gcagttttct gccgacgacc gttatcaaaa aaggcactgt aactgtcaac taaaggattt
    18061 tcgcctttat ggaacaccgc tgcgatcgct ttttgcttca gtaacggatg taattgtgcg
    18121 ccttcactgt tctgcacaca gtgatcgggc cagaaggttt gtggcaaacc gtcgagttgg
    18181 cctggcgtat aaggctctac accgtgctga ctggcaaaac tgccgtgatt cgccggatgc
    18241 cagtcctgac tggcgataac tgcttcaccg cgcgactggc accagtcaat caggcggtta
    18301 gcgacatcca ccgtactgtc accttccggc acggcgagcg cgccaccagc acagaaatca
    18361 ttttgtaaat cgaccagtaa cagggcgcga gggggcattg cagtctcctt aatcatccgg
    18421 cgtcagttcg ccacgcaggt tttggctcat ggccttgcga atggtttcag tatcaagttc
    18481 ctggctaagc aggtaatgca gtttggttag cgtggcttct acagtcatat ctgcaccgcc
    18541 aataacgccg gcatgggcga gggcgttacc ggtggcataa ccacccatgt tcactttacc
    18601 ggacatacat tgtgtcaggt tgaccaccac aataccgcga tcgctggctt cttgtaattc
    18661 ctgcaggaag gctttgtttt gtggcgcatt acccacgcca tatgagcgca gaatcaatgc
    18721 tttcacaggt tggcgcagaa aattgcgcac gacgtcagca gaaatccccg gataaatcgt
    18781 cactacgcca attggttgcg gcgtgattgg atgaacgatc aattcccctt cgccgtgcgg
    18841 ggcgggtggc gtattcaaac gacgaatatg gatacctgct tccagtaacg gaggaaggtt
    18901 tggagaggca aacgcatcaa aaccatcggc atgggctttg gtggttcggt tgccacgata
    18961 cagtcgatta ttaaaaaaga gagttacttc gttgatagga taattcgccg caacgtacaa
    19021 cgcattcagc agattaattt gtccgtcaga gcgtaactca gccagcggga tttgtgaccc
    19081 tgtcacaatg accggtttac tgagattctc gagcatgaac gacagtgcag aggcggtata
    19141 cgccatcgtg tctgtgccgt gcagaatgac aaaaccatca tagtcgtcat agtgcgcttt
    19201 aatatcttca gcaatatgct gccagtcttc tggcgtcata tcagaagaat ccatcaatgg
    19261 cgtatattca tgaatggtga aatctggcat ctccgggcga tggaattccg gcatcagcgc
    19321 cagttggcgc tgtagatgac ctgacaccgg aatataaccc tgctcggaac gctgcatccc
    19381 gatggtcccg cccgtgtagg cgacgtaaat tgatttcttt tgcatgatat tgatatgaat
    19441 gatatcgaaa gagcaaaagt atatacgtga ggggcagcga gcgcacctgg atactctgtg
    19501 gatatgagaa aagtccggca gaacggaggg attagtgtcc cgccggagaa aagtggttaa
    19561 tcctgcaacg cataggttac ggtggtgact acgcgtaccg ttttattaat cgccgtagga
    19621 tcatcttcat cactgccact ggcgaagatc tggaacacgc cttgtgaggc atcgctaatt
    19681 gaccccacct ggctacctga gtccgttgca aattgtaatg ctgcgttgcg tgcatctttg
    19741 gtggcggcgg caatcatctc agcgcgcagt gcgccaatac cattaaagcg ataggcgact
    19801 ttgctctcag cgatagcgcc ctccgtagcg acttgcagtt cgtttaattt cgcgacgact
    19861 ttttcgacag cagcaacatt atgggttttt atcgtcactg acccgtctgc gttatagcgt
    19921 ggcattgtgg gatcatcttt cagataaaag gaatctgttc gcttgcttgt ccattggccc
    19981 agggttatct ctttctcgtt aatcccctga gcattgagtt cggcaacaat ttttttctgc
    20041 gcgtcttcca gtttcgggaa cagttcgccg ggaatattac ccttaaagtt aatcgcaatt
    20101 gttaattctg ccgtatctgc gcgaacttct ttttcagaaa gcccacgcac atttacataa
    20161 cggttattgg tttttaaatg tttaacacca tcgccaacaa aataaccaca ggcgctaatc
    20221 cccagtccga ggacgatagc ggcaattaaa gagggggcct tattcactga tgactccata
    20281 tcagatgcta aatgagcaag ggaatgtagt ggagagaggg agtgaaagac agtccgattc
    20341 aggagtgtgg ccgacagcat atcggccaca tttttgcagg acttaacgca cgttggcgca
    20401 ggtcaggcaa aacgcataac ggttttgtgg gtcgttaaac gcggccagct tgtcgctttc
    20461 acttttaacg gtagaggcta ccgaggccag cggtgcaggt aacatggcct ggaacgcatc
    20521 tggcaacatt gcccggacag aaccagacat gttgtccatc actttgtcga agaaggtcgg
    20581 ttcattaacg tagtattcca gatgccactg tttcactttt gccagctctg ctgctttggc
    20641 gaccgcatca tcgaaatccc cgagactatc gaccagcccg ttagcttttg catcctgacc
    20701 ggtccagacg tggccctggg cgattttatc aatctgctcc ggcgttgaat gacgcgcatc
    20761 agcaaccagc gtgataaagc gtttatagcc attctcaatg cttaactgca tcatctgctg
    20821 cacttccggc ggcagtgccc tggtgataga aacatccgcc agcggtgaag ttgagacacc
    20881 atcagtatga acaccaatcg aatccagact attttctacg gtggtgatca cgccgaagat
    20941 accgatagaa ccggtcaggg tgctggggtt agccacaatg taattagctg gtgtggaaat
    21001 ccagtaacca ccagatgccg ccatgccacc catcgataca accacaggct tacccgctgc
    21061 ccgggctgct gccagttcag cgcgaatcac ttcagacgcg gtaacgctgc cgccgggact
    21121 attaacacgc agaacaatcg ctttcacttt cgggtcaagg cgagcgtcgc ggatttgtgc
    21181 cgcagtggta tcaccgccaa cattcccctg agtttcctcg ccatccataa ttgcgccatt
    21241 agcgaagacg acaccgatac tgtcaccggt atctgccggc gttttcaatg cgtaatcgta
    21301 ataactgatg gcgcgataat ttttatcagt tttactccag ccgaactctt tggtcagagt
    21361 tttttcgatt tcggcactcg atgccagtgc atcgaccagc ttgttttcca gcgcatattt
    21421 cgcggtatcg ccaccggttt tggttaaacc ctcaagcaac ccttgcgcgc cagggaatac
    21481 ctgctgagca gggatctggc ggttagcggc aacagtattc agatagtttt gccacagctc
    21541 accaatccag cggctgtcag cttcgcgggc tgccggcgac atatcatcac gaataaacgg
    21601 ttcaacggca gatttatacg tacccacgcg gaacacatgg gtggaaactt tcagcttatc
    21661 cagtaacgat ttgtagtaca gaccgttggt ggcaaagccg tgcagatcaa ccacgccttg
    21721 cggagacagc caaattttat tggcgaaact ggcgagataa tattgcccct ggctgtagtt
    21781 ctcgccaacg gcataaaccg gtttcccgct gtcacgaaac tctttcagag ctttgccgat
    21841 gtactgcata gacggctggt cgccgcctgc gaagtttttc agatccatca caataccggt
    21901 gatattgcgg tcgtccttcg cctggcgaat ggtgttgacg atatcaaaca gtgagttttc
    21961 ctgaagacga tcggaactgg caccaagcag ctggcggctt aatttactaa accgctgaga
    22021 actgtcgggt ttatcgacga tcacaccaga aatgtccagc agcagtgcgc cacgactggc
    22081 cgtttctttc gaatcaccac cactgacctg catccagatc cccacaccaa ccagcacgag
    22141 aaaaataaag aacaggttaa gtaccatttc acggacgaaa ttcagcagac gccacgtcca
    22201 tttaaaaaat ccggcaataa atcgccaaag ggttcgcatg tattctccca acttaaggtc
    22261 acacctgttt caggcgcaat agatgtggtt atcgtaatga cccaaccggc acttgtcagc
    22321 aggaattgcg gactgggctg taacaaaatc cactctcgtg ttaattttgt gaataaatat
    22381 catgacagga gttaatcaaa tggatgcact cgaactattg atcaatcgcc gtagcgcctc
    22441 ccgcctggct gaacccgcgc caacgggtga acaactgcaa aacatcctgc gtgcgggtat
    22501 gcgtgcgccg gaccataagt ccatgcaacc gtggcatttc tttgtgattg aaggggaagg
    22561 gcgcgagcgt ttcagcgccg tactggagca gggggcgatt gctgccggta gtgataacaa
    22621 agcaatcgac aaagcgcgta atgcgccgtt ccgcgcaccg ctcatcataa cggtggtggc
    22681 gaaatgtgaa gaaaatcata aagtcccgcg ctgggaacag gaaatgtctg cgggatgcgc
    22741 ggtcatggcg atgcaaatgg cagcagttgc ccaggggttt ggcggcatct ggcgcagtgg
    22801 tgcattaact gaaagtccgg tagtgcgtga agcattcggt tgccgtgagc aggataaaat
    22861 tgtcggtttt ctctacctcg gtacgccgca gttgaaagca tctacgtcga taaacgtccc
    22921 ggacccgacg tcgtttgtga cttatttctg ataattgtcg cgaaactgtc tggattgtga
    22981 gcaaaggcgc aggaattcag acactctcac ttatcacttc atggaatgag ggttaccata
    23041 cccggattgc attgacagga gatgtccatg agcgagaact cgattcgttt gacccaatac
    23101 agccacggcg ctggttgcgg ctgtaaaatt tccccaaaag tgttggaaac catcctgcac
    23161 agtgagcagg cgaagtttgt tgatccgaat ttgcttgtgg gtaatgaaac ccgcgacgat
    23221 gcggcggtgt acgatctggg caatggcacc agcgttatca gtaccaccga cttctttatg
    23281 ccgatcgttg ataatccttt cgattttggc cgcattgcgg cgactaacgc catcagcgat
    23341 atctttgcta tgggcggcaa accgattatg gcgattgcga tcctcggctg gccgattaac
    23401 aaactttccc cggaaattgc ccgcgaagtg accgaaggtg gacgctatgc atgccgtcag
    23461 gcgggtattg cgctggctgg cggtcactcc atcgatgcgc cggagccgat ttttggtctg
    23521 gcggtaacgg ggatcgtacc gaccgagcgg gtgaagaaaa acagtaccgc acaagccgga
    23581 tgcaaactgt tcctgacgaa accgctgggg attggcgttc ttaccacggc tgagaaaaaa
    23641 tcactgctga aaccagaaca tcagggactg gcgacggaag tgatgtgccg gatgaacatc
    23701 gcaggcgcgt cctttgccaa catcgaaggc gtaaaagcga tgaccgacgt tacgggtttt
    23761 ggtttactgg ggcatttaag cgaaatgtgt cagggcgcag gtgtgcaggc gcgcgtcgac
    23821 tatgacgcga tcccgaaatt gccgggggtt gaagagtaca ttaagttggg cgcagtgcct
    23881 ggcggcaccg aacgtaactt tgccagctac ggtcatctga tgggtgaaat gccgcgtgaa
    23941 gtgcgcgatc tgctgtgcga tccgcaaact tctggcggtt tgctgctggc ggtcatgccg
    24001 gaagcagaaa atgaggtcaa agctacagcc gccgagtttg gcattgaact gacggcgatt
    24061 ggcgaactgg tgccagcgcg cggcggtcgt gccatggttg agatccgtta attcaatgcg
    24121 gttgtttatt gccgaaaaac cgagtctggc gcgcgccatt gctgatgtcc tgcctaaacc
    24181 gcaccggaaa ggcgatggct ttatcgagtg cggtaatggt caggtggtga cctggtgtat
    24241 cggtcacctg cttgagcagg cgcagccaga cgcctacgac agccgctatg cgcgctggaa
    24301 tcttgcggat ttgccaattg tcccggaaaa gtggcaatta cagccccgac cctccgtgac
    24361 taaacaactt aatgtcatca aacgcttcct gcatgaagcc agcgaaatcg ttcacgccgg
    24421 agacccggat cgcgaagggc aactgctggt ggatgaagtg ctggactacc tgcaactggc
    24481 accggaaaag cgccagcagg tacagcgttg tttgataaac gacttgaacc cgcaggcggt
    24541 tgagcgggcg atcgatcgtc ttcgctccaa tagcgagttt gtaccgctgt gcgtttctgc
    24601 actggcgcgg gcgcgggctg actggctgta cggcattaat atgacccgtg cgtataccat
    24661 tctcggccgc aatgccggtt atcagggtgt gctttccgtg ggacgcgtgc agacgcccgt
    24721 actcggactg gtggtgcgcc gcgacgaaga gattgaaaac ttcgtggcga aagacttctt
    24781 tgaagtcaaa gcacatatcg tgacacctgc cgatgagcgg tttaccgcta tctggcaacc
    24841 gagcgaagcg tgtgaaccgt accaggatga agaagggcgc ttgttacatc gtccactggc
    24901 ggagcatgtg gttaaccgta ttagtggtca accagctatt gtcaccagct ataacgataa
    24961 acgggaatca gaatccgcgc cgctgccttt ttcgctttcg gcgttgcaga ttgaagcggc
    25021 taaacgtttt ggtctgagcg cgcagaacgt gcttgatatc tgccagaagc tgtacgaaac
    25081 gtacaagcta atcacttatc cgcgttccga ttgccgctat ttgccagaag aacattttgc
    25141 cggacgccac gcggtgatga atgctatcag cgttcatgca ccggatctgt tgccgcagcc
    25201 agtggtagat ccagatatac gcaaccgctg ttgggatgac aaaaaggtcg atgcgcacca
    25261 cgccatcatt ccgaccgcac ggagttctgc gatcaacctg acggagaacg aagcgaaggt
    25321 ctataacctg attgcccgtc agtacttgat gcagttctgc ccggatgcgg tgttccgcaa
    25381 gtgtgttatc gaactggaca ttgccaaagg caaatttgtc gctaaagccc gttttcttgc
    25441 tgaagcaggc tggcgcacgc tgttaggcag caaagagcgc gatgaagaaa acgacggcac
    25501 gccgctgcct gtggtggcga aaggcgatga attgttgtgc gaaaaaggcg aagtggtaga
    25561 gcggcaaacc cagccgccgc gccattttac cgatgcaaca ctgctttcgg cgatgaccgg
    25621 gatcgcgcgc tttgtgcagg acaaagatct gaaaaagatc cttcgtgcga ccgatggtct
    25681 ggggacagaa gcaacgcgtg ccgggattat tgaactgttg ttcaagcggg gtttcctgac
    25741 caaaaaaggg cgctatatcc actccaccga cgccggaaaa gcgctattcc attcgctgcc
    25801 agaaatggcg acgcgaccgg acatgaccgc gcactgggaa tcggtgctga cgcaaatcag
    25861 cgaaaagcag tgtcgctatc aggactttat gcagccgctg gtggggacat tgtaccagtt
    25921 gattgatcag gcaaagcgca cgtcggtaag gcagtttcgg ggaataatgg ctcccggcgg
    25981 tggagaaggg aagaaaaagg attcgccacg caagagagcg ccgaaaaaaa gcccgccatc
    26041 agaagaggcg ggcaatggag tgataatata aaggttcttt caaccatctg atactgaaga
    26101 tttttatggc cggataaggc gtttgcgctg tatccggcac aattgtctta aatcacgccc
    26161 tggcttaaca tcgcatccgc aaccttcaca aaaccggcaa tgttcgcgcc ctgcacgtag
    26221 ttggtttgct caccttcacc accatgctca acacaggcat ggtggatatc cagcatgatg
    26281 tgatgcaaac gtgcgtcaac tttctcggct ttccagccca ggcgcgcagc gttttgcgcc
    26341 atttccaggc ccgatgttgc tacaccgcca gcattagccg ctttacccgg tgcaaacagt
    26401 acgcctgcct gctggaacag ttcagtcgct tcgatggtgg tcggcatatt tgccccttcg
    26461 gcgacggctt taacgccatt ggcgataagc tgatgcgcgg cgtcaacatc cagttcgttc
    26521 tgggtggcgc aaggcagggc gatatcaacc ggtacagacc acggttgttg gccttcgaga
    26581 tagaccagac caaattcttt ggcgtaatct gccactcgac catcgcggct ggctttgatt
    26641 tcgataaggc gcgccagttt ctctttcgtg aatccgcttt catcaactac agtgccgctg
    26701 gagtctgacg cagtgatcac tcgagcacca aattccatcg ctttttcgat agcgtactgg
    26761 gcgacgttgc cggagccaga aacggaaacg cgcatccctt caaaacccat accgtggcgt
    26821 tttagcattg cttctgtgaa ataaaccaga ccgtagccgg tagcttccgg gcgaataaga
    26881 ctgccgccaa atgaaaggcc cttaccggtg aagacgcagg cggtattgtt ggagagcttt
    26941 ttcatcatcc ccgccataaa gccgacttca cgaccaccaa cgccgatatc acctgccgga
    27001 acgtcagtat ccgcgcccag gtggcgatac agttcagtca tcagcgcctg gcagaaacgc
    27061 ataacttcgc cttcgctttt ccctttcgga tcaaagtcgc tgccgccttt accaccaccc
    27121 atcggcagag tagtcagggc atttttaaag gtttgctcga agccgaggaa tttgagaatg
    27181 gaaaggttta ctgaagggtg gaagcgcata ccgcctttgt acggaccgat ggcggagctg
    27241 aattgcacac gccatgcacg gttgacctgc acctggttgc gatcatcaac ccatactacg
    27301 cgaaattgga tcacgcgctc cggttcaacc agacgctcca gtaatgacat ctgacgatat
    27361 ttgggatttt gttcaagaaa aggccagagt gtggtcatta cttcacgaac ggcttgcgcg
    27421 aactcggttt gattcgggtc gcgcttttgg acatggtt
//
LOCUS       DAHHTG010000060        26722 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.060, whole genome shotgun sequence.
ACCESSION   DAHHTG010000060 DAHHTG010000000
VERSION     DAHHTG010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26722)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26722)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26722
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.060"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            62..793
                     /gene="artJ"
                     /locus_tag="NBI74_002462"
     CDS             62..793
                     /gene="artJ"
                     /locus_tag="NBI74_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein ArtJ"
                     /protein_id="HCD1932784.1"
                     /translation="MKKLVLAALLASFTFGASAAEKINFGVSATYPPFESIGANNEIV
                     GFDIDLAKALCKQMQAECTFTNHAFDSLIPSLKFRKYDAVISGMDITPERSKQVSFTT
                     PYYENSAVVIAKKDTYKTFADLKGKRIGMENGTTHQKYIQDQHPEVKTVSYDSYQNAF
                     IDLKNGRIDGVFGDTAVVNEWLKTNPQLGVATEKVTDPQYFGTGLGIAVRPDNKALLE
                     KLNNALAAIKADGTYQKINDQWFPQ"
     gene            complement(822..1949)
                     /gene="rlmC"
                     /locus_tag="NBI74_002463"
     CDS             complement(822..1949)
                     /gene="rlmC"
                     /locus_tag="NBI74_002463"
                     /EC_number="2.1.1.189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(747)-C(5))-methyltransferase
                     RlmC"
                     /protein_id="HCD1932785.1"
                     /translation="MQCALYDAGRCRSCQWITQPIPEQLSAKTADLKNLLADFPVEEW
                     CAPVSGPEQGFRNKAKMVVSGSVEKPLLGMLHRDGTPEDLCDCPLYPASFAPVFAALK
                     PFIARAGLTPYNVARKRGELKYILLTESQSDGGMMLRFVLRSDTKLAQLRKALPWLQE
                     QLPQLKVITVNIQPVHMAIMEGETEIYLTEQQALAERFNDVPLWIRPQSFFQTNPAVA
                     SQLYATARDWVRQLPVKHMWDLFCGVGGFGLHCATPDMQLTGIEIAPEAIACAKQSAA
                     ELGLTRLQFQALDSTQFATAQGEVPELVLVNPPRRGIGKPLCDYLSTMAPRFIIYSSC
                     NAQTMAKDIRELPGYRIERVQLFDMFPHTAHYEVLTLLVKQ"
     gene            complement(1990..2478)
                     /locus_tag="NBI74_002464"
     CDS             complement(1990..2478)
                     /locus_tag="NBI74_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjO family protein"
                     /protein_id="HCD1932786.1"
                     /translation="MEDETLGFFKKTSSSHARLNVPALVQVAALAIIMIRGLDVLMIF
                     NTLGVRGIGEFIHRSVQTWSLTLVFLSSLVLVFIEIWCAFSLVKGRRWARWLYLLTQI
                     TAASYLWAASLGYGYPELFSIPGESKREIFHSLMLQKLPDMLILMLLFVPSTSRRFFQ
                     LQ"
     gene            complement(2538..3383)
                     /gene="potI"
                     /locus_tag="NBI74_002465"
     CDS             complement(2538..3383)
                     /gene="potI"
                     /locus_tag="NBI74_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotI"
                     /protein_id="HCD1932787.1"
                     /translation="MNNLPVVRSPWRIVILLLGFTFLYAPMLMLVIYSFNSSKLVTVW
                     AGWSTRWYGELLRDDAMMSAVALSLTIAACAATAAAILGTIAAVVLVRFGRFRGSNGF
                     AFMITAPLVMPDVITGLSLLLLFVALAHAIGWPADRGMLTIWLAHVTFCTAYVAVVIS
                     SRLRELDRSIEEAAMDLGATPLKVFFVITLPMIMPAIISGWLLAFTLSLDDLVIASFV
                     SGPGATTLPMLVFSSVRMGVNPEINALATLILGAVGIVGFIAWYLMARAEKQRIRDIQ
                     RARRG"
     gene            complement(3380..4324)
                     /gene="potH"
                     /locus_tag="NBI74_002466"
     CDS             complement(3380..4324)
                     /gene="potH"
                     /locus_tag="NBI74_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotH"
                     /protein_id="HCD1932788.1"
                     /translation="MSTLEPAAQPKPFKLWLSQLQMKHGRKLVIALPYIWLILLFLLP
                     FLIVFKISLAEMARAIPPYTELMEWADGQLSITLNLGNFLQLTDDPLYFDAYLQSLQV
                     AAISTICCLLIGYPLAWAVAHSKPSTRNILLLLVILPSWTSFLIRVYAWMGILKNNGV
                     LNNFLLWLGIIDQPLTILHTNFAVYIGIVYAYVPFMVLPIYTALIRIDYSLVEAALDL
                     GARPLKTFFTVIVPLTKGGIIAGSMLVFIPAVGEFVIPELLGGPDSIMIGRVLWQEFF
                     NNRDWPVASAVAIVMLLLLIMPIMWFHKHQQKSEGEHG"
     gene            complement(4334..5467)
                     /gene="potG"
                     /locus_tag="NBI74_002467"
     CDS             complement(4334..5467)
                     /gene="potG"
                     /locus_tag="NBI74_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter ATP-binding subunit
                     PotG"
                     /protein_id="HCD1932789.1"
                     /translation="MNDAIPRPQAKTRKALTPLLEIRNLTKSYDGQHAVDDVSLTIYK
                     GEIFALLGASGCGKSTLLRMLAGFEQPSAGQIMLDGVDLSQVPPYLRPINMMFQSYAL
                     FPHMTVEQNIAFGLKQDKLPKAEIASRVNEMLGLVHMQEFAKRKPHQLSGGQRQRVAL
                     ARSLAKRPKLLLLDEPMGALDKKLRDRMQLEVVDILERVGVTCVMVTHDQEEAMTMAG
                     RIAIMNRGKFVQIGEPEEIYEHPTTRYSAEFIGSVNVFEGVLKERQEDGLVLYSPGLV
                     HPLKVDADASVVDNVPVHVALRPEKIMLCEEPPANGCNFAVGEVIHIAYLGDLSVYHV
                     RLKSGQMISAQLQNAHRHRKGLPTWGDEVRLCWEVDSCVVLTV"
     gene            complement(5562..6674)
                     /gene="potF"
                     /locus_tag="NBI74_002468"
     CDS             complement(5562..6674)
                     /gene="potF"
                     /locus_tag="NBI74_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotF"
                     /protein_id="HCD1932790.1"
                     /translation="MTALNKKWLSGLVAGALMAVSVSTLAAEQKTLHIYNWSDYIAPD
                     TVANFEKETGIKVVYDVFDSNEVLEGKLMAGSTGFDLVVPSASFLERQLTAGVFQPLD
                     KSKLPEWKNLDPELLKLVAKHDPDNKFAMPYMWATTGIGYNVDKVKAVLGENAPVDSW
                     DLILKPENLEKLKSCGVSFLDAPEEVFATVLNYLGKDPNSTKADDYTGPATDLLLKLR
                     PNIRYFHSSQYINDLANGDICVAIGWAGDVWQASNRAKEAKNGVNVSFSIPKEGAMAF
                     FDVFAMPADAKNKDEAYQFLNYLLRPDVVAHISDHVFYANANKAATPLVSAEVRDNPG
                     IYPPADVRAKLFTLKVQDPKIDRVRTRAWTKVKSGK"
     gene            complement(7024..7500)
                     /locus_tag="NBI74_002469"
     CDS             complement(7024..7500)
                     /locus_tag="NBI74_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HCD1932791.1"
                     /translation="MTSLVVPGLDTLRQWLDDLGMSFFECDNCQALHLPHMQNFDGVF
                     DAKIDLIDNTILFSAMAEVRPSAVLPLAADLSAINASSLTVKAFLDMQDDNLPKLVVC
                     QSLSVMQGVTYEQFAWFVRQSEEQISMVILEANAHQLLLPTDDEGQNNVTENYFLH"
     gene            complement(7588..8490)
                     /gene="rimK"
                     /locus_tag="NBI74_002470"
     CDS             complement(7588..8490)
                     /gene="rimK"
                     /locus_tag="NBI74_002470"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6--L-glutamate ligase"
                     /protein_id="HCD1932792.1"
                     /translation="MKIAILSRDGTLYSCKRLREAAIQRGHLVEILDPLSCYMNINPA
                     ASSIHYKGRKLPHFDAVIPRIGTAITFYGTAALRQFEMLGSYPLNESVAIARARDKLR
                     SMQLLARQGIDLPVTGIAHSPDDTSDLIDMVGGAPLVVKLVEGTQGIGVVLAETRQAA
                     ESVIDAFRGLNAHILVQEYIKEAQGCDIRCLVVGDEVVAAIERRAKEGDFRSNLHRGG
                     AASVASITPQEREIAIKAARTMALDVAGVDILRANRGPLVMEVNASPGLEGIEKTTGI
                     DIAGKMIRWIERHATTEYCLKTGG"
     gene            complement(8551..9273)
                     /gene="nfsA"
                     /locus_tag="NBI74_002471"
     CDS             complement(8551..9273)
                     /gene="nfsA"
                     /locus_tag="NBI74_002471"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase NfsA"
                     /protein_id="HCD1932793.1"
                     /translation="MTPTIELICGHRSIRHFTDEPISEAQREAIINSARATSSSSFLQ
                     CSSIIRITDKALREELVTLTGGQKHVAQAAEFWVFCADFNRHLQICPDAQLGLAEQLL
                     LGVVDTAMMAQNALTAAESLGLGGVYIGGLRNNIEAVTELLKLPQHVLPLFGLCLGWP
                     ADNPDLKPRLPASILVHENSYQPLDKDALAQYDEQLAEYYLTRGSNNRRDTWSDHIRR
                     TIIKESRPFILDYLHKQGWATR"
     gene            complement(9257..9544)
                     /locus_tag="NBI74_002472"
     CDS             complement(9257..9544)
                     /locus_tag="NBI74_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1418 family protein"
                     /protein_id="HCD1932794.1"
                     /translation="MRAIGKLPKSVLILEFIGMMLLAVALLSVSDSLSLPEPFSRPEV
                     QILMIFLGVLLMLPAAVVVILQVAKRLAPQLMNHPPQYSRSEREKDNDANH"
     gene            9704..9961
                     /gene="grxA"
                     /locus_tag="NBI74_002473"
     CDS             9704..9961
                     /gene="grxA"
                     /locus_tag="NBI74_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 1"
                     /protein_id="HCD1932795.1"
                     /translation="MQTVIFGRPGCPYCVRAKDLAEKLSNERDDFQYQYVDIRAEGIT
                     KEDLQQKAGKPVETVPQIFVDQQHIGGYTDFAAWVKENLDA"
     gene            complement(9991..10368)
                     /locus_tag="NBI74_002474"
     CDS             complement(9991..10368)
                     /locus_tag="NBI74_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YbjM"
                     /protein_id="HCD1932796.1"
                     /translation="MKHKQRWAGAICCFVLFIVVCLFLATHMKGAFRAAGHPEIGLLF
                     FILPGAVASFFSHRREVLKPLFGAMLAAPCSMLIMRLFFSPTRSFWQELAWLLSAVFW
                     CALGALCFLFISSLFKPQHRKNQ"
     gene            10638..12323
                     /locus_tag="NBI74_002475"
     CDS             10638..12323
                     /locus_tag="NBI74_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate:alanine antiporter"
                     /protein_id="HCD1932797.1"
                     /translation="MNINVAELLNGNYILLLFVVLALGLCLGKLRLGSIQLGNSIGVL
                     VVSLLLGQQHFSINTDALNLGFMLFIFCVGVEAGPNFFSIFFRDGKNYLMLALVMVGS
                     ALVIALGLGKLFGWDIGLTAGMLAGSMTSTPVLVGAGDTLRHSGMESRQLSLALDNLS
                     LGYALTYLIGLVSLIVGARYLPKLQHQDLQTSAQQIARERGLDTDANRKVYLPVIRAY
                     RVGPELVAWTDGKNLRELGIYRQTGCYIERIRRNGILANPDGDAVLQMGDEIALVGYP
                     DAHARLDPSFRNGKEVFDRDLLDMRIVTEEVVVKNHNAVGKRLAQLKLTDHGCFLNRV
                     IRSQIEMPIDDNVVLNKGDVLQVSGDARRVKTIADRIGFISIHSQVTDLLAFCAFFVI
                     GLMIGMITFQFSTFSFGMGNAAGLLFAGIMLGFMRANHPTFGYIPQGALSMVKEFGLM
                     VFMAGVGLSAGSGINNGLGAIGGQMLIAGLIVSLVPVVICFLFGAYVLRMNRALLFGA
                     MMGARTCAPAMEIISDTARSNIPALGYAGTYAIANVLLTLAGTIIVMVWPGLG"
     gene            complement(12499..13035)
                     /gene="rcdA"
                     /locus_tag="NBI74_002476"
     CDS             complement(12499..13035)
                     /gene="rcdA"
                     /locus_tag="NBI74_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RcdA"
                     /protein_id="HCD1932798.1"
                     /translation="MRRANDPQRREKIIQATLEAVKLYGIHAVTHRKIATLAGVPLGS
                     MTYYFSGIDELLLEAFSRFTEIMSRQYQAFFSDVSDAPGACQAITDMIYSSQVATPDN
                     MELMYQLYALASRKPLLKTVMQNWMQRSQQTLEQWFEPGTARALDAFIEGMTLHFVTD
                     RKPLSREEILRMVERIAG"
     gene            13119..14327
                     /locus_tag="NBI74_002477"
     CDS             13119..14327
                     /locus_tag="NBI74_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCD1932799.1"
                     /translation="MTVNSSRNALKRRTWALFMFFFLPGLLMASWATRTPAIRDILSV
                     SIAEMGGVLFGLSIGSMSGILCSAWLVKRFGTRNVILVTMSCALIGMMILSLALWLTS
                     PLLFAVGLGVFGASFGSAEVAINVEGAAVEREMNKTVLPMMHGFYSLGTLAGAGVGMA
                     LTAFGVPATVHILLAALVGIAPIYIAIQAIPDGTGKNAADGTQHGEKGVPFYRDIQLL
                     LIGVVVLAMAFAEGSANDWLPLLMVDGHGFSPTSGSLIYAGFTLGMTVGRFTGGWFID
                     RYSRVAVVRASALMGALGIGLIIFVDSSWVAGVSVVLWGLGASLGFPLTISAASDTGP
                     DAPTRVSVVATTGYLAFLVGPPLLGYLGEHYGLRSAMLVVLALVILAAIVAKAVAKPD
                     TKTQTAMENS"
     gene            14327..15142
                     /gene="ybjI"
                     /locus_tag="NBI74_002478"
     CDS             14327..15142
                     /gene="ybjI"
                     /locus_tag="NBI74_002478"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase"
                     /protein_id="HCD1932800.1"
                     /translation="MSIKLIAVDMDGTFLSDQKTYNRERFMAQYQQMKAQGIRFVVAS
                     GNQYYQLISFFPEIANEIAFVAENGGWVVSEGKDVFNGELSKDAFATVVEHLLTRPEV
                     EIIACGKNSAYTLKKYDDAMKTVAEMYYHRLEYVDNFDNLEDIFFKFGLNLSDELIPQ
                     VQKALHEAIGDIMVPVHTGNGSIDLIIPGVHKANGLRQLQKLWGIDDSEVVVFGDGGN
                     DIEMLRQAGFSFAMENAGSAVVAAAKYRAGSNNREGVLDVIDKVLKHEAPFKQ"
     gene            15228..15512
                     /locus_tag="NBI74_002479"
     CDS             15228..15512
                     /locus_tag="NBI74_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1932801.1"
                     /translation="MIMKNCLLLGALLMGFTGVAMAQSVTVDVPSGYKVVVVPDSVSV
                     PQAVSVATVPQTVYVAPAPAPAYRPHPYVRHLASVGEGMVIEHQIDDHHH"
     gene            complement(15553..16785)
                     /gene="mdfA"
                     /locus_tag="NBI74_002480"
     CDS             complement(15553..16785)
                     /gene="mdfA"
                     /locus_tag="NBI74_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdfA"
                     /protein_id="HCD1932802.1"
                     /translation="MQNKLASGARLGRQALLFPLCLVLYEFSTYIGNDMIQPGMLAVV
                     EQYQAGIDWVPTSMTAYLAGGMFLQWLLGPLSDRIGRRPVMLAGVVWFIVTCLAILLA
                     QNIEQFTLLRFLQGISLCFIGAVGYAAIQESFEEAVCIKITALMANVALIAPLLGPLV
                     GTAWIHVLPWEGMFVLFAALAAISFFGLQRAMPETATRIGEKLSLKELGRDYKLVLKN
                     GRFVAGALALGFVSLPLLAWIAQSPIIIITGEQLSSYEYGLLQVPIFGALIAGNLLLA
                     RLTSRRTVRSLIIMGGWPIMIGLLVAAAATVISSHAYLWMTAGLSIYAFGIGLANAGL
                     VRLTLFASDMSKGTVSAAMGMLQMLIFTVGIEISKHAWLNGGNGLFNLFNLVNGILWL
                     SLMVIFLKDKQMGNSHEG"
     gene            17070..17666
                     /gene="ybjG"
                     /locus_tag="NBI74_002481"
     CDS             17070..17666
                     /gene="ybjG"
                     /locus_tag="NBI74_002481"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HCD1932803.1"
                     /translation="MLENLNLSLFSLINATPDSAPWMISLAIFIAKDLITVVPLLAAV
                     LWLWGLTAQRQLVIKIAIALAVSLFVSWTMGHLFPHDRPFVENIGYNFLHHAADDSFP
                     SDHGTVIFTFALAFLCWHRLWSGSLLMVLAVVIAWSRVYLGVHWPLDMLGGLLAGMIG
                     CLSAQIIWQAMGHKLYQRLQSWYRFCFALPIRKGWVRD"
     gene            17724..18482
                     /gene="deoR"
                     /locus_tag="NBI74_002482"
     CDS             17724..18482
                     /gene="deoR"
                     /locus_tag="NBI74_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor DeoR"
                     /protein_id="HCD1932804.1"
                     /translation="METRREERIGQLLQELKRSDKLHLKDAAALLGVSEMTIRRDLNN
                     HSAPVVLLGGYIVLEPRSASHYLLSDQKSRLVEEKRRAAKLAATLVEPDQTLFFDCGT
                     TTPWIIEAIDNEIPFTAVCYSLNTFLALKEKPHCRAFLCGGEFHASNAIFKPIDFQQT
                     LNNFCPDIAFYSAAGVHVSKGATCFNLEELPVKHWAMSMAQKHVLVVDHSKFGKVRPA
                     RMGDLKRFDIVVSDCCPEDEYVKYAQTQRIKLMY"
     gene            complement(18529..19731)
                     /gene="dacC"
                     /locus_tag="NBI74_002483"
     CDS             complement(18529..19731)
                     /gene="dacC"
                     /locus_tag="NBI74_002483"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HCD1932805.1"
                     /translation="MTQYSSLLRGLAAGSAFLFLFAPTAFAAEQTVEAPSVDARAWIL
                     MDYASGKVLAEGNADEKLDPASLTKIMTSYVVGQALKADKIKLTDMVTVGKDAWATGN
                     PALRGSSVMFLKPGDQVSVADLNKGVIIQSGNDACIALADYVAGSQESFIGLMNGYAK
                     KLGLTNTTFQTVHGLDAPGQFSTARDMALLGKALIHDVPEEYAIHKEKEFTFNKIRQP
                     NRNRLLWSSNLNVDGMKTGTTAGAGYNLVASATQGDMRLISVVLGAKTDRIRFNESEK
                     LLTWGFRFFETVTPIKPDATFVTQRVWFGDKSEVNLGAGEAGSVTIPRGQLKNLKASY
                     TLTEPQLTAPLKKGQVVGTIDFQLNGKSIEQRPLIVMENVEEGGFFGRMWDFVMMKFH
                     QWFGSWFS"
     gene            19978..20604
                     /gene="gstB"
                     /locus_tag="NBI74_002484"
     CDS             19978..20604
                     /gene="gstB"
                     /locus_tag="NBI74_002484"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308945.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase GstB"
                     /protein_id="HCD1932806.1"
                     /translation="MITLWGRNNSTNVKKVLLTLEELELPYEQILAGREFGINHDADF
                     LAMNPNGLVPLLRDDESDLILWESNAIVRYLAAQYGQKRLWIDSPARRAEAEKWMDWA
                     NQTLSNAHRGILMGLVRTPPEERDQAAIDASCKECDALFALLDAELAKVKWFSGDEFG
                     VGDIAIAPFIYNLFNVGLTWTPRPNLQRWYQQLTERPAVRKVVVIPVS"
     gene            complement(20601..21716)
                     /gene="yliI"
                     /locus_tag="NBI74_002485"
     CDS             complement(20601..21716)
                     /gene="yliI"
                     /locus_tag="NBI74_002485"
                     /EC_number="1.1.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose sugar dehydrogenase YliI"
                     /protein_id="HCD1932807.1"
                     /translation="MHRQSFFLVPLICLSSALWAAPATVNVEVLQDKLDHPWALAFLP
                     DNHGMLITLRGGELRHWQAGKGLSAPLSGVPDVWAHGQGGLLDVVLAPDFAQSRRIWL
                     SYSEVGDDGKAGTAVGYGRLSDDLSKVTDFSTVFRQMPKLSTGNHFGGRLVFDGKGYL
                     FIALGENNQRPTAQDLDKLQGKLVRLTDLGEIPDDNPFVKESGVRAEIWSYGIRNPQG
                     MAMNPWSNALWLNKHGPRGGDEINIPQKGKNYGWPLATWGINYSGFKIPEAKGEIVAG
                     TEQPVFYWKDSPAVSGMAFYNSDKFPQWQQKLFIGALKDKDVIVMSVNGDKVTEDGRI
                     LTDRGQRIRDVRTGPDGYLYVLTDESSGELLKVSPRN"
     gene            complement(21827..22210)
                     /gene="bssR"
                     /locus_tag="NBI74_002486"
     CDS             complement(21827..22210)
                     /gene="bssR"
                     /locus_tag="NBI74_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssR"
                     /protein_id="HCD1932808.1"
                     /translation="MFVDRQRIDLLNRLIDARVDLAAYVQLRKAKGYMSVSESNHLRD
                     NFFKLNRELHDKSLRLNLHLDQEEWSALHHAEEALATAAVCLMSGHHDCPTVITVNAD
                     KLENCLMSLTLSIQSLQKHAMLEKA"
     gene            22423..23748
                     /gene="rimO"
                     /locus_tag="NBI74_002487"
     CDS             22423..23748
                     /gene="rimO"
                     /locus_tag="NBI74_002487"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HCD1932809.1"
                     /translation="MSKVTPQPKIGFVSLGCPKNLVDSERILTELRTEGYDVVPSYDD
                     ADMVIVNTCGFIDSAVQESLEAIGEALNENGKVIVTGCLGAKEDQIREVHPKVLEITG
                     PHSYEQVLEHVHHYVPKPKHNPFLSLVPEQGVKLTPRHYAYLKISEGCNHRCTFCIIP
                     SMRGDLVSRPIGEVLSEAKRLVDAGVKEILVISQDTSAYGVDVKHRTGFHNGEPVKTS
                     MVSLCEQLSKLGIWTRLHYVYPYPHVDDVIPLMAEGKILPYLDIPLQHASPRILKLMK
                     RPGSVDRQLARIKQWREICPELTLRSTFIVGFPGETEEDFQMLLDFLKEARLDRVGCF
                     KYSPVEGADANALPDQVPEEVKEERWNRFMQLQQQISAERLQEKVGREILVIIDEVDE
                     EGAIGRSMADAPEIDGAVYLNGETNVKPGDILRVKVEHADEYDLWGSRV"
     gene            complement(23795..25123)
                     /locus_tag="NBI74_002488"
     CDS             complement(23795..25123)
                     /locus_tag="NBI74_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HCD1932810.1"
                     /translation="MSRINKFVLTVSLLIFIMISAVACGIYTQMVKERVYGLKQSVID
                     TAFAVANIAEYRRSVAIDLINTLNPTEEQLLVGLRTAYADSVSPSYLYDVGPYLISSD
                     ECIQVKEFEKNYCADIMQVVKYRHVKNTGFISFDGKTFVYYLYPVTHNRSLIFLLGLE
                     RFSLLSKSLAMDSENLMFSLFKNGKSVTGDEYNAKNAIFTVSEAMEHFSYLPTGLYVF
                     AYKKDVYFQVCTLIIFFAALVAVISGASCLYLVRRVINRGIVEKEAIINNHFERVLDG
                     GLFFSAADVKKLYSMYNSAFLDDLTKAMGRKSFDEDLKALPEKGGYLCLFDVDKFKNI
                     NDTFGHLLGDEVLMKVVKILKSQIPVDKGKVYRFGGDEFAVIYTGGTLEELLSILKEI
                     VHFQVGSINLSTSIGVAHSNECTTVERLKMLADERLYKSKKNGRAQISWQ"
     gene            complement(25131..26570)
                     /locus_tag="NBI74_002489"
                     /pseudo
     CDS             complement(25131..26570)
                     /locus_tag="NBI74_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001435936.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
BASE COUNT         6835 a         6925 c         6384 g         6578 t
ORIGIN      
        1 ttgttatccc atgccgcaga cacggccaaa aatcataaga ttgacagacg ggagttccat
       61 catgaaaaag ttagttcttg ccgctttact tgcttctttt actttcggtg cttctgccgc
      121 agagaaaatc aattttggcg tttcagccac ctatccaccc tttgaatcta taggtgctaa
      181 taatgagatt gtcggctttg atatcgacct ggcaaaagcc ttgtgcaaac aaatgcaggc
      241 agaatgtact tttactaatc acgctttcga cagcctgatc ccgtcactga aattcagaaa
      301 atatgacgcc gtaatctccg gaatggatat cacgccggag cgtagcaaac aggtatcgtt
      361 taccacgccc tactacgaaa actcagccgt cgtgattgcc aaaaaagata cctacaaaac
      421 gtttgccgat ctgaaaggca aacgtattgg gatggaaaac ggcaccacgc accagaaata
      481 tattcaggat cagcacccgg aagtgaaaac tgtctcctat gacagttatc agaatgcctt
      541 tatcgatctg aaaaatggcc gtattgatgg cgtatttggt gacaccgcag tggtaaacga
      601 atggctgaaa accaatccgc aactgggcgt tgctactgaa aaagtgaccg atccgcaata
      661 cttcggcacc ggcctgggta tcgctgtacg tccggataac aaagctctgc tggaaaaact
      721 gaataacgcg ctggcagcaa ttaaagctga cggtacatat cagaaaatca atgaccagtg
      781 gttcccacag taagattcag aaagccgcac ctgcggcttt tttattgctt caccagtagc
      841 gtcagcactt catagtgcgc ggtgtgcggg aacatatcaa aaagctgtac ccgttcgatg
      901 cgataaccag gcagttcgcg gatatctttc gccatagttt gggcgttaca gctggagtag
      961 ataataaaac gcggtgccat cgttgagaga taatcacaca gcggtttacc gatgccgcgg
     1021 cgcggcgggt taaccagcac cagctccggc acttccccct gagcggtggc aaactgcgta
     1081 gagtccagcg cctgaaattg caaacgcgtt aagcccagtt cagcggctga ctgcttcgca
     1141 caggcaatgg cctctggtgc aatttcgatc ccggttaact gcatgtcagg cgtcgcgcag
     1201 tgtaaaccaa agccacccac accgcagaac agatcccaca tatgtttaac cggcagctgt
     1261 cgtacccagt cgcgcgcggt ggcgtacagc tggctggcga ccgccggatt agtctggaag
     1321 aaactttgcg gacggatcca cagcggtacg tcattaaaac gctctgccag tgcctgttgt
     1381 tcggtcaggt agatctccgt ctccccttcc ataatcgcca tatgtaccgg ctgaatattg
     1441 acggtaataa ctttcagctg cggtaattgt tcctgtaacc acggcagcgc cttacgcaat
     1501 tgcgccagtt tggtatcaga acgcagtaca aaacgcagca tcatgccacc atcgctctgg
     1561 ctttcagtca gcagaatgta tttcagttca ccacgtttac gcgccacgtt gtagggcgtt
     1621 aaccccgcac gggcgataaa cggttttagc gccgcaaaaa cgggcgcaaa tgaggctgga
     1681 taaagcgggc agtcacaaag gtcttccggt gtaccatctc gatgcagcat accgagcagt
     1741 ggtttttcaa cgctaccact caccaccatt ttggctttat tacgaaaccc ttgttccggg
     1801 cctgacaccg gcgcacacca ttcctcaacc ggaaaatcgg cgagcagatt tttaagatcg
     1861 gcagttttag cggagagttg ctctggaatc ggctgcgtta tccactgaca ggaacgacag
     1921 cgacccgcgt cgtaaagtgc gcactgcata cattgacctt cacatcatca ggggcgacga
     1981 ttatacacat tattgcaact ggaagaaccg ccgactggtc gagggaacga acagcagcat
     2041 caggatgagc atatccggca gcttctgcag catcaggcta tggaagattt cacgttttga
     2101 ttcaccggga atgctgaaca gctccggata accatacccc agcgaagccg cccacaagta
     2161 acttgcggct gtgatttgtg tcaacagata cagccagcgc gcccagcgac gccctttcac
     2221 cagtgaaaac gcacaccaga tctcaatgaa aaccagcacc agactgctca aaaagaccag
     2281 cgttaaactc caggtttgta cgctgcgatg aatgaactcg ccaataccgc gcacgcccag
     2341 cgtattgaaa atcatcagca cgtcgaggcc acggatcata ataatggcga gcgccgccac
     2401 ctgcaccagc gcaggcacat tcaggcgagc atgagatgaa gatgttttct taaaaaatcc
     2461 caacgtttcg tcttccatga aaacaatgcc gcgacatgcg cggcattatg tagccaggtt
     2521 ggcaaatttt agtgtcttca gccacgtctt gcacgctgga tgtcgcgtat ccgctgtttt
     2581 tctgcgcgcg ccatcagata ccaggcgata aatccgacaa ttccgaccgc gccgaggatc
     2641 aatgttgcca gggcgttgat ttccggattc acccccatcc gcacgctgga aaagaccagc
     2701 atcggtaatg tggtggctcc cggcccggaa acaaagctgg cgatcaccag atcatcaagc
     2761 gacaaagtaa aagccagtaa ccagccagaa atgatcgcgg gcatgatcat cggtagcgta
     2821 atgacaaaaa acactttcag cggcgtcgca ccgagatcca tcgcggcttc ttctatcgag
     2881 cgatccagtt cccgcagacg cgacgaaata acgactgcca cataagccgt acagaaagtg
     2941 acatgcgcca gccagatggt gagcataccg cggtccgcag gccagccaat agcatgagca
     3001 agcgcgacga ataacaacaa cagcgacaaa cccgtgatga catctggcat taccagcggt
     3061 gcggtgatca tgaaggcaaa accatttgat ccgcgaaacc tgccaaagcg caccagcacg
     3121 accgccgcaa tagtcccgag gatcgccgcc gccgtcgccg cacaggccgc aattgtcaga
     3181 cttaaagcaa ccgcactcat catcgcgtca tcgcgcaata attcgccata ccagcgcgtt
     3241 gaccagccgg cccacaccgt cactagcttc gagctgttaa acgaatagat aacaagcatc
     3301 agcattggcg cgtagagaaa ggtgaagccc aacagcaaaa tcacaatccg ccagggcgaa
     3361 cgaaccaccg gtaaattatt catccgtgtt ctccctcgct tttttgctga tgtttgtgga
     3421 accacattat cggcataatt agcagcagca acatgacgat cgctaccgct gaggccaccg
     3481 gccagtcgcg gttgttaaag aactcctgcc acagcacgcg cccgatcatg atgctgtccg
     3541 ggccaccgag cagttccggg atcacaaact cgcccacagc cgggataaac accagcatcg
     3601 atccggcaat aatcccacct ttagtcagcg gcacgatcac agtaaagaac gttttcagcg
     3661 gtcgtgcacc gagatccagt gccgcttcta ccagcgaata atcaatacga atcaacgcgg
     3721 tgtaaatcgg cagcaccata aacggcacgt aggcgtaaac aatgccaata taaaccgcaa
     3781 agttggtatg cagaatggtc agcggttgat cgataatccc cagccacagc agaaaattat
     3841 tcagcacacc gttgtttttt aatattccca tccaggcgta gacacgaatc agaaacgagg
     3901 tccacgacgg caggatcacc agtagtaata agatattacg ggtcgaaggc ttactgtgcg
     3961 ccaccgccca cgccagcgga tagccgatca gtaaacagca aatagtcgaa atcgccgcca
     4021 cctgtaacga ctggagataa gcatcaaaat agagcggatc gtcggtcagt tgcagaaaat
     4081 taccgagatt aagagtgata gaaagttgcc cgtcagccca ctccatcaat tcggtataag
     4141 gtggaatagc gcgcgccatc tccgccaggc ttattttaaa gacgatcaga aatggcagca
     4201 gaaacagcaa gatcaaccag atatacggca acgcaataac cagtttgcgc ccatgtttca
     4261 tttgcagctg cgacaaccac agcttaaagg gttttggctg ggcagcaggt tcaagtgtac
     4321 tcatctttgc tccttaaacc gtcagcacca cacagctgtc cacttcccag cacaaacgca
     4381 cttcgtcgcc ccaggtcggt aaccctttac gatgacgatg ggcgttttgt agctgggcgc
     4441 taatcatctg cccacttttc agacgaacgt gatacaccga aagatcgccg agataggcaa
     4501 tgtgtatcac ctcccccacc gcgaagttac aaccattggc gggcggttcc tcgcaaagca
     4561 tgattttttc cgggcgaagc gccacatgca ccggcacgtt atcgaccact gaagcatccg
     4621 catcgacttt cagtggatgc accagccccg gcgaataaag caccagacca tcttcctgac
     4681 gctctttgag tacaccttca aagacattaa ccgaaccgat aaattcagcg ctgtagcgag
     4741 tggtcggatg ctcgtagatc tcttccggct cgccaatctg gacaaatttc ccacgattca
     4801 taatggcgat gcgccccgcc atagtcatcg cctcttcctg atcgtgagta accattacgc
     4861 aggtcacacc gacgcgctcc agaatatcca ccacttcaag ctgcatccgg tcacgcagct
     4921 ttttatccag cgcgcccatc ggctcatcga gcagtaatag tttcgggcgc ttggcaaggc
     4981 ttctggccag ggccacacgc tgacgctgac caccagaaag ctgatgcggt ttgcgtttgg
     5041 cgaactcctg catatgcacc agcccaagca tctcattgac ccggctggca atttccgctt
     5101 tcggtagttt gtcctgtttc aggccaaaag cgatgttctg ttccacggtc atatgcggaa
     5161 acagcgcgta agactgaaac atcatattga tggggcgcag gtaaggcggc acctgtgaca
     5221 aatcgacgcc atcgagcata atctgcccgg ccgaaggttg ttcaaaacct gccagcatac
     5281 gcagcagcgt ggacttgcca cagccggatg cgcccagcag cgcgaagatt tcacctttgt
     5341 agatggtcag gctgacatca tccaccgcat gttgaccatc gtaggatttg gtcaggttac
     5401 ggatttctaa tagcggcgtc agcgccttac gggttttcgc ctgcgggcga gggatagcgt
     5461 cattcacggc tgctctccgg cattgaatgt gtgcaagcgc accatgatgg tgcgaattgc
     5521 tggcgggtgt ggtgcgcccc tccggcatct acggctgcgg attattttcc gctcttcact
     5581 ttggtccagg cgcgggtgcg cacacggtcg attttcgggt cctgcacttt cagagtgaac
     5641 agcttcgcac gaacatccgc aggcggataa atacccgggt tatcacggac ttccgcactc
     5701 accagcggcg tggctgcttt attggcgtta gcatagaaca catggtcaga gatatgtgcc
     5761 accacatcag gacgcagcag gtaattcagg aactgatagg cttcgtcttt gtttttggca
     5821 tccgcaggca tggcgaatac atcaaagaac gccatcgccc cttcttttgg aatcgagaac
     5881 gagacattca cgccattctt cgcttccttc gcgcggtttg acgcctgcca gacatcgccc
     5941 gcccagccaa tagcgacaca gatgtcacca ttcgccagat cattgatgta ttgggatgaa
     6001 tggaaataac gaatgttcgg gcgcagcttt aacagcagat cggtagccgg tccggtgtaa
     6061 tcatccgctt tagtgctgtt aggatctttg ccgagataat tcagcacggt agcaaacact
     6121 tcttctggcg catccaggaa agagacaccg cagcttttca gtttttccag attttcaggt
     6181 ttgaggatca agtcccagct atcgacgggc gcgttttcgc ccagcaccgc tttaacttta
     6241 tcgacgttat agccaatccc ggtcgtcgcc cacatatagg gcatagcaaa tttattgtcg
     6301 ggatcgtgtt tggcgaccaa cttcagcagc tccggatcga gattcttcca ctccggcaat
     6361 ttgcttttat ccagcggctg gaaaactccc gctgtcagct ggcgctcaag aaagcttgct
     6421 gacggcacca ccagatcaaa gccggtgctc cccgccatta atttgccttc cagtacttcg
     6481 ttagaatcga aaacatcata gacgacttta ataccggttt ctttttcaaa gttggccacc
     6541 gtgtccgggg cgatataatc agaccagtta taaatgtgga gtgttttttg ttcagccgcg
     6601 agcgtgctga cagaaacggc catcagagca cccgcaacca gacccgatag ccatttttta
     6661 tttaaggcgg tcatatctct ttccttctga aaattcgtta acaaacgaac aaaagttgtc
     6721 acactgaaag atacgcaata aacgtgcata tttttatcgg cgcggtgtaa gaaaagagaa
     6781 ctctctcccg gcctgcactg ctcactatat aaaagcatcg caagaataac tttagccagg
     6841 gtttgagact atagctcgga ttaaaaaacg gtatttaaca aatttttatg cgtatttgct
     6901 ctatgtgata aagccaaatc ggcacaagag aagtgaatta atctttaata aaaggttaaa
     6961 atcagcggat aattttgcgg cacgcagtca ctgcggcagc gactgcgtgc tcgaaagaag
     7021 ttatcagtgg aggaaatagt tttcggtaac gttattttgc ccttcatcgt cagtcggtaa
     7081 cagcagttga tgggcattag cttcaagaat gaccatcgaa atctgctctt cgctctgacg
     7141 cacgaaccat gcaaactgct cataggttac gccctgcata acggataaag actggcaaac
     7201 caccagcttt ggcagattat catcctgcat atcaagaaat gctttcacgg tcagcgaact
     7261 ggcattgatg gctgataaat ccgccgccag cggcaatacg gctgaaggtc ggacttccgc
     7321 catggcagaa aacaggatcg tgttatcgat cagatcgatt ttagcatcaa agacaccgtc
     7381 gaaattctgc atatggggca gatgcagagc ctgacagtta tcacattcaa aaaaactcat
     7441 ccccaggtca tcgagccatt gacgcaacgt atccagacca ggaacgacca gcgatgtcat
     7501 aattttgtac aacctcttcc gataaaaaga ccggcacagc ttacgcaaaa agcgcaggca
     7561 aaaccatgat cagtaatgtg attgcgacta accacccgtt ttcaggcaat attctgtcgt
     7621 agcgtggcgt tcgatccagc ggatcatttt acccgcgatg tcgataccgg tggttttttc
     7681 tattccttcc agccctggcg acgcattcac ttccatcacc aacggcccgc gattagcacg
     7741 cagaatatcc acaccagcaa cgtccagcgc catcgttcgc gcggctttta tcgcgatttc
     7801 acgctcctgt ggtgtgatac tggcgacgct tgccgcgccg ccacgatgca aattggaacg
     7861 aaaatcgccc tctttcgccc gccgttcaat cgcagcgact acttcatcgc caacaaccag
     7921 acagcggata tcgcaccctt gcgcctcttt gatatattcc tgcaccagaa tatgcgcgtt
     7981 cagaccgcgg aaagcgtcaa tcacgctttc cgccgcctga cgcgtctccg ccagcacgac
     8041 gccaattccc tgcgtgcctt caaccagctt gaccaccagc ggcgcaccac cgaccatgtc
     8101 gattaaatcg ctggtatcat ccggcgaatg cgcaatgccc gtgacaggca ggtcgatgcc
     8161 ctgacgcgcc agcagttgca tggaacgcaa tttgtcacgc gcccgggcaa tggcgaccga
     8221 ctcattgagc ggatagctcc ccagcatctc gaactggcgc agtgccgccg tcccataaaa
     8281 ggtaatggcg gtgccaatac gcgggatcac tgcgtcaaaa tggggtaact tgcggccttt
     8341 gtagtgaata gaagacgccg caggatttat gttcatatag caagaaagcg gatcaagaat
     8401 ttcaaccagg tgaccgcgct gtatagcggc ttcacgcagc cgcttacacg aatagagcgt
     8461 tccatcccgg gacaatatgg caattttcac cctgcacctc tctgtcagac ctggtcaaaa
     8521 gcccgcgtat catacatcga cgtggcagtt ttagcgcgtc gcccaaccct gtttgtgcaa
     8581 ataatccaga ataaatgggc ggctttcttt aatgattgtt cggcggatat gatcgctcca
     8641 ggtatcccgg cgattattgc tgccacgggt gaggtaatat tccgccagtt gctcgtcata
     8701 ctgcgccagt gcgtctttat ccagcggttg atagctgttt tcatgcacca aaatggaggc
     8761 cggtaaacgc ggcttaagat ccggattatc cgcaggccag ccaaggcaca gcccaaacag
     8821 cggcagaaca tgctgcggta atttcagcag ttccgtcacc gcttcaatat tattgcgcag
     8881 gccgccgata tataccccgc ccaatcccag cgattccgct gcggttaatg cattctgcgc
     8941 catcattgcc gtatcaacga caccgagcaa cagttgttcc gccaggccga gctgagcatc
     9001 cggacagatc tgtaaatggc ggttaaagtc ggcacagaac acccaaaact ctgccgcttg
     9061 cgctacgtgt ttttgcccgc cggtcagcgt caccagttct tcacgtaacg ctttgtcggt
     9121 aatgcggata atgctactgc actgcaaaaa actggaactg gacgtcgcac gggcgctgtt
     9181 aataatcgcc tcacgctgcg cttcggaaat gggttcatca gtgaaatggc gaatggagcg
     9241 atggccacaa ataagttcaa tggttggcgt cattatcttt ttctctttct gaacgtgaat
     9301 attgcggtgg atggttcatc agctgtgggg caagacgttt tgccacctga agaataacca
     9361 ccaccgcagc gggaagcatg agcaaaacac cgagaaaaat catcagaatc tgcacttctg
     9421 gccgagaaaa tggctcaggt agcgacaggg agtcgcttac cgacagcagc gccaccgcca
     9481 gcaacatcat tccgataaat tccagtatca acacgctttt aggcaattta ccgatcgcgc
     9541 gcatacgctt ccctctgcaa agtgagcctt cagtctaaaa cttttcactg tattgtgttt
     9601 aacagttata gcttttagca attaatgcaa caggttaaac ctactttcag cgaatacatt
     9661 ttagcgtgat cattacaggc ataaatctat gaggagagaa ataatgcaaa ccgttatttt
     9721 tggtcgtccg ggttgccctt actgtgtgcg tgcaaaagat ctggctgaga aattgagcaa
     9781 tgaacgcgat gatttccagt atcagtatgt agatattcgt gcggaaggga tcactaaaga
     9841 agatctacaa caaaaggcag gtaaacccgt agaaaccgtg ccgcagattt ttgtcgatca
     9901 gcaacatatc ggcggctata ccgattttgc tgcatgggtg aaagagaatc tggacgcctg
     9961 atcgtctgac aagccctcgc attgagggct ttattgattt tttctgtgct gtggtttaaa
    10021 caaactactg ataaataaga aacacaatgc ccccagcgca caccagaaca ccgcacttaa
    10081 taaccatgcc agctcttgcc agaatgagcg cgtcggtgaa aaaaacagcc gcataatgag
    10141 catcgagcag ggtgccgcca gcattgcgcc aaacagaggt ttcaggactt ctctacgatg
    10201 tgaaaagaag ctggcaactg caccaggaag aatgaaaaat agcaaaccga tttcaggatg
    10261 cccggcagcc cgaaaagcgc ctttcatgtg cgtcgccaga aaaagacaca ccacaatgaa
    10321 gaggacaaaa cagcagattg cccccgccca acgttgttta tgtttcactc gttcctcctg
    10381 acactgcgtc tatcgaacac atttttcgcc agtgtggcgt tcagtaagat aaagccgctt
    10441 cgcattccat gctaatatag gccaacgcaa ttcatatagc cgttgatacc taatgtgatt
    10501 acactagtaa aatatattgt tactttacta tcgtttaggt gcgctgaatg aatctgcgcc
    10561 ctgaattctg gtaaaaaaca ttatcgtaaa ttaccatttc tttcaacagc ttactagtaa
    10621 acaagaagtt agcctccgtg aatataaacg tcgccgaatt gttaaatggg aattacattc
    10681 tgttattatt tgtggtcctc gcgcttgggc tatgtctcgg gaaattacga cttggttcga
    10741 tccaactggg taattccatt ggcgttttag tcgtatcgct gttattaggc caacaacatt
    10801 tcagcattaa caccgacgcg cttaatcttg gctttatgct gtttattttc tgcgtcgggg
    10861 tcgaagccgg accgaacttt ttttccattt tttttcgcga tgggaaaaat tacctaatgt
    10921 tagcactggt gatggttggc agtgcgctgg tgatcgcctt agggttaggt aagctgtttg
    10981 gctgggatat tggcctgacg gccggtatgt tagctggctc tatgacgtcg acaccggttc
    11041 tggtcggtgc tggcgataca ctgcgtcatt ccggcatgga aagcaggcag ctctcactgg
    11101 cactggataa tctgagcctc gggtatgcct taacctattt aatcggtctg gtgagtttga
    11161 ttgttggtgc gcgttacttg ccgaaattgc agcatcagga cttacagacc agcgcccagc
    11221 aaatcgcccg cgaacgtggc ctggacactg atgccaaccg taaggtttat ttaccggtga
    11281 tccgcgccta ccgcgtcggc ccggagctgg tggcctggac cgacggcaaa aatctgcgtg
    11341 aactgggtat ttatcgacaa actggctgct acattgaacg tattcgacgt aacgggattc
    11401 tggcaaatcc agacggtgat gcggtgctac aaatgggcga tgaaatagcg ttggtaggct
    11461 atcccgacgc ccacgcccga ctcgatccca gcttccgtaa cggcaaagaa gttttcgatc
    11521 gtgaccttct cgacatgcgt atcgtcactg aagaagtggt cgttaaaaac cataacgccg
    11581 ttggcaaacg tctcgcacaa ctgaagttga ccgatcacgg ttgcttcctt aaccgcgtca
    11641 ttcgtagcca gattgagatg ccgatagatg acaacgtcgt gcttaacaaa ggtgacgttt
    11701 tacaagtcag cggcgatgcc cgtcgcgtaa aaaccatcgc cgatcgcatc ggctttatct
    11761 cgattcatag ccaggtcact gacttgctgg cattctgcgc cttctttgtt attgggctga
    11821 tgatcgggat gatcaccttc cagttcagca cattcagttt cggcatggga aacgctgccg
    11881 ggttgttatt cgccggaatt atgctgggat ttatgcgcgc taaccacccg accttcggtt
    11941 acattccgca gggtgcatta agcatggtga aagagttcgg cttaatggtg tttatggcag
    12001 gcgttggtct gagcgccggt agcggtatta ataacggcct gggcgcgatt ggcggtcaga
    12061 tgttgattgc cggattgatt gtcagtcttg tgccagtggt tatctgtttc ttgttcggtg
    12121 cttatgtatt gcgaatgaac cgcgcactgt tgttcggcgc aatgatgggc gcacgcacct
    12181 gcgcgccggc aatggagatc atcagtgata cagctcgcag taacatcccg gcgctgggct
    12241 atgcgggcac ctatgcaatc gccaacgtcc tgctgacgct ggcagggaca atcatcgtca
    12301 tggtatggcc aggattagga taaaactgaa gttgccctga aaatgaaatt ttttttgcac
    12361 aaccgcagaa cttttccgca gggcatcagt cttaattagt gccactgctt ttctttgatg
    12421 tccccatttt gtggagccca tcaaccccgc catttcggtt caaggttgat gggttttttg
    12481 ttgcctgaaa tttatctact acccagcaat cctctcaacc atcctcaaaa tctcctcgcg
    12541 tgatagcggc ttacggtcgg tgacaaaatg cagcgtcatc ccctcaataa acgcatcaag
    12601 cgcgcgggcg gttccgggtt caaaccattg ttcgagcgtt tgttgactgc gctgcatcca
    12661 gttttgcatt accgttttta atagcggttt tcggctagcc agcgcgtaga gctggtacat
    12721 cagctccata ttatccggcg ttgcaacctg tgagctgtag atcatatcgg tgatagcctg
    12781 gcatgcgcct ggagcatcac taacatcgct aaaaaatgcc tgatattgcc gggacatgat
    12841 ctcagtaaaa cggctgaacg cctccagcaa caactcatca attcctgaaa aatagtaggt
    12901 catcgatccc aacggcaccc cggcaagggt agcaattttg cggtgtgtaa cagcatgtat
    12961 tccgtaaagt ttcaccgcct ccagcgtggc ctggataatt ttttctcgcc gttgcggatc
    13021 gttagcgcga cgcatgtcat ttcctctctc tgcaattgtg tacaaatgta cacagtcttg
    13081 ctaatgttgt cttccctctt cttattttga cgtggtctat gaccgtaaat tcttcacgta
    13141 atgcattgaa acgccgaacc tgggcgctgt ttatgttctt ctttttgcca ggcctgttaa
    13201 tggcgtcctg ggcaacccgt acgcctgcta tccgcgatat tctctctgtc tcgatcgctg
    13261 aaatgggcgg tgttctcttt ggtctgtcga tcggttcgat gagcggtatt ctctgctcag
    13321 cgtggttagt gaaacgcttt gggacgcgta atgtcatcct tgtcacgatg tcctgcgcat
    13381 tgattgggat gatgatacta agtctggcac tctggctgac atcgcccttg ctctttgccg
    13441 ttggtctcgg cgtctttggg gcaagttttg gttctgcgga agtggcgata aacgttgaag
    13501 gtgccgccgt tgagagagaa atgaataaga cggttttacc gatgatgcac ggtttttata
    13561 gcctgggcac gctggcaggc gctggtgtcg ggatggcact gacggccttt ggcgttccgg
    13621 caacggtgca cattttattg gcggcgctgg taggcatcgc gcctatttat atcgccattc
    13681 aggcaatccc tgacggtacg ggcaaaaatg ctgccgatgg cacccagcat ggtgaaaaag
    13741 gcgtaccttt ttatcgcgat atccagttgc tgctgattgg tgttgtggtg ctggcgatgg
    13801 cctttgccga aggttctgcc aacgactggt tacccttatt aatggttgac ggtcacggtt
    13861 ttagccctac ttccggctca ctgatttatg ccggttttac cctggggatg actgttggac
    13921 gcttcaccgg cggttggttc atcgaccgtt acagtcgcgt tgccgtggtt cgggccagtg
    13981 cactaatggg ggcgttgggt attgggctga ttatttttgt cgatagctcc tgggtcgcag
    14041 gagtgtctgt tgtactttgg ggactgggtg cctcgctggg cttcccgctg accatttctg
    14101 ccgccagcga taccggcccc gatgcaccaa cccgcgtcag tgtggtagca acgaccggtt
    14161 atctggcttt cctcgtcggg ccgccgctgc tgggctatct cggcgaacat tatggattac
    14221 gtagtgcaat gctggttgta ctggcgctgg ttattctcgc ggctattgtc gcgaaagccg
    14281 tcgccaaacc cgataccaaa acgcagacgg cgatggagaa tagttgatga gcattaaatt
    14341 aattgcggta gacatggatg gtactttctt aagcgatcaa aaaacctata accgtgagcg
    14401 gtttatggct cagtatcagc aaatgaaagc acaaggcatt cgctttgtgg tcgccagcgg
    14461 gaatcaatat tatcagttga tctctttctt ccctgaaatt gctaatgaaa tagcctttgt
    14521 ggctgaaaac ggcggctggg tagtgagcga aggcaaagat gtttttaatg gcgagctgtc
    14581 gaaggatgcg tttgctactg tcgtggaaca tttgctgacg cgcccggaag tggaaattat
    14641 tgcctgcgga aaaaatagcg cctataccct caaaaaatat gacgatgcca tgaaaacggt
    14701 ggcggaaatg tattatcacc gtctggaata cgtcgataac tttgacaact tagaagatat
    14761 cttctttaag tttggtctga atctttccga tgaactgatc ccacaagtgc aaaaagcatt
    14821 acatgaggcc atcggcgata tcatggtacc ggtccatacc ggcaacggca gcatcgatct
    14881 gattatcccc ggtgtacata aagccaatgg ccttcgccaa ctgcagaaat tatggggaat
    14941 agacgacagc gaagtggtgg tctttggcga tggcggtaac gatattgaga tgctgcgtca
    15001 ggcaggcttt agttttgcaa tggaaaatgc cggcagcgcg gtcgtcgcag cggcaaaata
    15061 ccgggcaggc tccaataacc gtgaaggcgt actggatgtg atcgataaag tgcttaaaca
    15121 cgaagcgcca tttaagcaat aaaaaaggca gcacgcccgg caacacaata cctgaatgaa
    15181 ggctgcataa ttcgcggaaa ttaacccgct gcattatttc aggcgaaatg attatgaaaa
    15241 attgtctact gttgggcgcg cttttaatgg gctttactgg tgtggcgatg gcgcaaagtg
    15301 tcaccgtcga tgtgccgagt ggatacaaag tggttgtggt tcctgattca gtcagcgttc
    15361 cacaagcggt cagcgtcgcg actgtgccgc aaaccgtcta tgtggctcct gcgccagctc
    15421 cggcttaccg cccgcatcct tatgttcgcc atcttgccag cgtaggtgaa gggatggtta
    15481 tcgaacacca gattgacgac catcaccatt aattcgagaa tgcctgatcg cacaaagcaa
    15541 tcaggcattt ttttaccctt cgtgagaatt tcccatctgt ttatctttta aaaagataac
    15601 catcagcgac agccacaaaa tgccgttgac aaggttgaag agattaaaca gtccgttgcc
    15661 cccgttcagc caggcatgtt tgctgatttc aataccgacg gtaaagatca gcatttgcag
    15721 catccccatc gccgcagaaa ccgtaccttt actcatatcg ctggcaaaca gggttaatcg
    15781 caccagtccc gcattcgcca gaccaatacc gaaagcataa atacttaacc cggcggtcat
    15841 ccataaatac gcgtgcgatg agataaccgt tgccgcagca gcgaccaaca gaccaatcat
    15901 aatcggccag ccacccataa taatcagcga acgtacggtg cggcgcgagg tcagacgcgc
    15961 taacagcaag ttacccgcaa ttaacgcccc gaaaataggc acttgcagca agccatattc
    16021 atagctgctc aactgctcac cggtaataat gataatcggc gactgggcga tccacgccaa
    16081 caacggcagg ctaacgaatc ccagcgccag cgcccccgcc acaaagcggc cgttcttcag
    16141 caccaactta tagtcacgac cgagttcttt cagcgacagt ttctcgccta tacgcgtggc
    16201 ggtttcaggc atagctcgtt gcagaccgaa aaaggagatc gctgccaatg cggcaaacaa
    16261 gacaaacatc ccttcccagg gcagcacatg gatccacgcc gtgcccacca gcggaccaag
    16321 tagtggagca atcagcgcca cgttcgccat tagcgcggtg atcttgatac aaacggcctc
    16381 ttcgaaggat tcctgaattg cggcgtatcc cacagcgcca atgaaacaga ggcttatgcc
    16441 ctgcaagaag cgcaacaggg tgaattgttc aatgttttgc gccagtaata ttgccagaca
    16501 ggtgacgata aaccacacca ctcccgccaa catcaccgga cggcggccaa tacgatccga
    16561 caacggcccc aggagccatt gtaaaaacat cccgcccgcc agatacgcag tcatcgaagt
    16621 aggaacccaa tcaatgcccg cctgatattg ttccaccacg gccaacatac cgggttgaat
    16681 catatcgttg ccgatatagg ttgaaaattc gtaaagcacc agacagagag ggaaaagtaa
    16741 cgcctgacgt ccaagcctgg caccggaagc taatttattt tgcatgcaat ttcttcgcca
    16801 ataataatcg cgcaaagttt aataaaagcg cagctaacga gaaagcgaat tttgtagctg
    16861 aaaccacggt taagcacatt cttacattat tgcgagtata gctacgcttt ctttaagttt
    16921 tatttaacct ttgcccgtta caatcaccta ccgtaaacag gccgcttgag ggaaataaga
    16981 cgatgccgct ttacccagtt taacctgcac tttattctca acgacttgcc tgtattggct
    17041 cccttttaat cactttgcgt cgggaagtta tgctggaaaa tttgaatctc tctctattct
    17101 ctcttattaa cgcaacgcca gactctgcgc cgtggatgat ctcgttggcg atttttattg
    17161 ctaaagattt gattaccgtg gtgccgttgc tggccgcagt actttggttg tgggggctta
    17221 cagcacaacg gcaactggtg ataaaaatcg ctatcgcgct ggcggtcagc ctgtttgtgt
    17281 cctggacgat gggacatctt tttccgcacg accgaccctt tgtcgaaaat atcggctata
    17341 acttcctgca tcatgcggcg gatgactcat tcccgagcga tcacggtacg gtgattttca
    17401 cctttgcact ggcattttta tgctggcatc gcctgtggtc cggctcactt ttaatggtgc
    17461 tggccgtcgt cattgcctgg tcgcgcgttt atcttggcgt ccactggccg ctggatatgc
    17521 tcggtggatt gctggcaggt atgattggct gccttagcgc ccagattatc tggcaagcga
    17581 tggggcataa actctatcaa cgtctgcaat cgtggtatcg cttctgtttt gcattaccga
    17641 tccgcaaagg ctgggtgcgt gactgatctc tgctaaaaag tgtagtattg agcggctcgc
    17701 ttcaataact attcagaggg attatggaaa cacgtcgcga agagcgtatc gggcagctgc
    17761 tgcaagaatt aaaacgcagc gataagttac atcttaaaga cgccgccgcc ctgcttgggg
    17821 tttcggagat gacgattcgt cgcgatctga acaaccacag tgcgcccgtc gttttgctcg
    17881 gcggctatat tgttctggaa ccacgcagcg ccagccatta cctgttaagc gatcaaaaat
    17941 cccgcctggt ggaagaaaaa cgccgggcgg caaaactggc tgcgacgctg gtagaacccg
    18001 atcagaccct cttttttgac tgtggcacca ccacgccgtg gattattgaa gcaattgata
    18061 atgaaatccc ttttaccgcc gtttgttatt cgctaaatac ttttctggcg ctgaaagaga
    18121 aaccccattg ccgcgcgttt ctttgcggtg gtgaatttca cgccagcaac gccattttca
    18181 aacccatcga ttttcagcaa acgctgaata atttttgccc ggatatcgct ttttattccg
    18241 ctgcgggcgt gcatgtcagt aaaggcgcta cctgctttaa tcttgaagag ttgccggtaa
    18301 aacactgggc catgtcgatg gcgcaaaagc atgtgctggt tgtcgaccac agtaaatttg
    18361 gcaaggtgcg tccggcgcgc atgggtgacc tgaaacgctt tgatattgtt gtgagcgatt
    18421 gttgcccgga agatgagtat gtgaagtacg cgcagacgca gcgtatcaag ttgatgtatt
    18481 aatgacgtat aaccggatga cgtttcgcgc catccggtta tcagaagatt aagagaacca
    18541 actgccgaac cactgatgga atttcatcat cacgaaatcc cacatccgac caaagaatcc
    18601 gccctcttcc acattttcca tcacgatcag cggacgctgc tcaatggatt taccgttaag
    18661 ctggaaatca atggtgccga caacctgacc ttttttcagc ggtgcggtaa gctgcggttc
    18721 cgttaacgta taactcgcct tcaggttttt cagctgccca cgcggtatgg tcactgagcc
    18781 cgcttcgcct gccccgagat tcacttcgct cttatcacca aaccagacgc gctgagtcac
    18841 aaatgtggca tcaggtttaa ttggcgtcac ggtttcaaag aagcggaaac cccaggtcaa
    18901 taatttctca gactcattaa aacggatacg gtcagttttc gcccccagca ctacggagat
    18961 taaacgcata tcgccctggg tagccgaagc aaccagatta tatcccgcgc ctgccgtggt
    19021 tcctgtcttc atgccatcaa cattcagatt gctgctccac agcagacgat tacggttagg
    19081 ctgacgaatt ttgttgaagg tgaactcttt ctctttatga atggcgtact cttccggcac
    19141 atcgtggatc aatgctttac ccagcaatgc catatcgcgc gcggtactga actgccccgg
    19201 cgcatccagg ccgtgcaccg tctggaaggt agtgttggtc agacccagtt ttttggcata
    19261 accattcatc aaaccaataa atgactcctg gctcccggca acgtaatcag ccagcgcaat
    19321 acaggcgtca ttaccggact ggataatcac acctttgttc aagtctgcca ccgaaacctg
    19381 atcgcccggt ttgaggaaca ttaccgatga accacgcagt gccggatttc ccgtcgccca
    19441 ggcatcttta ccgaccgtca ccatatcggt gagtttaatt ttatcggcct taagcgcctg
    19501 cccaaccaca tagctggtca tgattttagt caggctcgcg ggatccagtt tctcatccgc
    19561 attgccttct gccagcactt taccgctggc gtaatccatt aaaatccatg cacgggcatc
    19621 cacgctcggc gcttcaacgg tttgttccgc cgcgaatgcc gttggggcaa aaaggaataa
    19681 aaatgcagaa cccgctgcaa gaccacgaag gagagaggag tattgcgtca taaatgccac
    19741 ccgagtatcc attcaaaaaa aatacgttat atcgccgtat gataagttcg gtgattaata
    19801 acgcacacac aaacttaaag aaaccgtaag ttggtaaagt ttttaaagtt tatgcaatat
    19861 cctgccgctt cgctgcttct ggtgtcattt ttttgccagc tgttgcttaa aagttaactt
    19921 tacttcacca tcggagtcct gttcagcctg tggctgcctg tcggaaaagg ggtgagtatg
    19981 attacgctgt ggggtcggaa taattcaacc aacgtaaaaa aagtattgct gacgctcgaa
    20041 gaactggaac taccttatga gcaaattctc gcgggccgtg agtttgggat aaatcacgat
    20101 gctgattttc tggcgatgaa ccctaacggg ctggtgccgt tgttgcgtga cgacgaaagt
    20161 gatctcattc tttgggaatc aaacgccatt gtccgctatc tggcagcaca gtacgggcaa
    20221 aaacgcctgt ggatcgactc accggcacgt cgtgcggaag cagaaaaatg gatggactgg
    20281 gcaaaccaga cgctcagtaa tgctcatcgc gggatcctga tgggattagt cagaacacca
    20341 ccggaagagc gcgatcaggc cgccattgat gccagttgca aagagtgcga cgccctgttt
    20401 gccctgctcg atgcggaact ggcaaaagta aaatggttct caggcgacga gtttggtgtg
    20461 ggcgatatcg ctatcgcacc gttcatctac aatttgttta acgttggcct gacctggaca
    20521 ccgcgtccaa atctgcaacg ctggtatcag caactcactg aacgccccgc agtgcgcaaa
    20581 gtggtggtga ttcccgttag ctaattgcgt gggctaactt taagtaattc cccactggac
    20641 tcgtcggtga gaacgtataa ataaccgtcg ggtccagtgc gaacatcacg aattcgctgc
    20701 cctctgtccg ttaaaatacg gccatcttct gtcactttgt cgccgttgac gctcatcaca
    20761 atgacatctt tatctttcag cgcgccaata aataattttt gctgccactg ggggaattta
    20821 tcgctgttat agaaggccat gccgctcacg gcgggcgaat ctttccagta aaaaacaggt
    20881 tgctcggtcc cggcgacgat ctcccctttc gcctccggta tcttaaagcc tgaatagttg
    20941 attccccagg ttgccagcgg ccagccgtag tttttgcctt tttgcgggat attaatttca
    21001 tcgccaccgc gcgggccatg tttattcagc catagtgcat tactccacgg attcatcgcc
    21061 attccttgcg gattacgaat gccataagac cagatctcgg cgcgcacacc ggattccttt
    21121 acaaaaggat tatcatccgg gatttcgccc aggtcggtca gacgcaccag tttgccctgt
    21181 aatttatcca gatcctgcgc cgtcgggcgc tgattgtttt cgcccagagc aataaaaaga
    21241 taacctttac cgtcgaatac cagccgcccg ccaaaatggt tgccggtaga cagttttggc
    21301 atctggcgaa agacggtgct gaagtcggtc acttttgaga gatcatcact taagcggcca
    21361 taacccacag cagttccggc tttgccatca tcgccaactt cggaataact taaccagata
    21421 cggcgcgact gagcaaaatc gggcgctaaa accacgtcca gcaggccgcc ctgcccgtgc
    21481 gcccaaacgt ccggcactcc ggaaagcggc gcagataatc cttttcctgc ttgccagtga
    21541 cgcaactcgc cgccgcgcag agtgattaac ataccgtgat tatcgggtaa aaaggccagc
    21601 gcccagggat ggtcgagttt gtcttgcagt acttcgacat ttaccgttgc aggagctgcc
    21661 cagagagcgg aagaaagaca aataaggggc acaaggaaaa aggattgtcg atgcataacg
    21721 cctccggatt gactctggct taaagcgtag tcagtggagg agataacaaa ttcattttta
    21781 caaaaactta aacatgaagg gggagacgct ttctccccct tagttttcag gccttctcaa
    21841 gcatggcgtg cttctgcagg ctctggatac tcagcgttaa gctcatcaga caattttcaa
    21901 gcttatcggc gttgacggta ataacagtcg ggcaatcatg gtgcccactc atcaaacata
    21961 ctgcggctgt cgctaatgct tcttcagcat gatgaagagc actccactct tcctgatcca
    22021 gatgaagatt caaccgcagc gatttatcgt gcagttcgcg attcagttta aaaaagttat
    22081 ctcgtagatg attgctttcg ctgacggaca tgtatccttt tgccttcctc agttgcacgt
    22141 atgcggcgag gtcaacgcgt gcgtcgatca accggttcag cagatcgatt cgctgtctgt
    22201 caacgaacat acttcgatcc tcctctttcc agccttgttg caactaaata gtatggctgt
    22261 tttttgagca aaatgctgtc caggatcaat tcttcacgcc gaattaacaa aatttacatt
    22321 tccttcaact ccgccagcga atgagattgc gatacaaatt ttatccagaa ctctgtagaa
    22381 tccctgccct gacctttcct gtaactgaat attttttaag ctatgagcaa agtaactccc
    22441 cagccgaaaa tcggctttgt ttcccttggc tgtccgaaaa accttgtcga ttcagagcgt
    22501 attctcaccg aactacgcac tgaaggttat gacgtggtac cgagctatga tgatgcggac
    22561 atggtgatcg tcaacacctg cggctttatt gacagcgcgg tacaagaatc actggaagcc
    22621 attggtgaag cgttgaatga aaacggcaag gtaattgtga ccggttgtct gggggcaaaa
    22681 gaagatcaga tccgcgaagt ccacccgaaa gtgctggaaa tcaccgggcc tcatagctat
    22741 gagcaggttc tggagcacgt tcatcactac gtgccaaaac cgaaacacaa cccattcctg
    22801 agcctggtgc cagaacaagg tgtgaaactg acgccgcgtc attatgccta tctgaaaatt
    22861 tctgaaggct gtaatcaccg ctgcaccttc tgcattattc cgtctatgcg cggcgatctg
    22921 gtgagccgtc cgattggcga agtgttaagt gaagcgaaac gtctggttga tgcgggcgtt
    22981 aaagaaattc tggtgatctc gcaggatact tccgcgtatg gcgtcgatgt taaacatcgt
    23041 actggcttcc acaacggcga gccggtaaaa accagcatgg tcagcctgtg cgaacagtta
    23101 tcgaaactgg ggatctggac acgtctgcac tacgtttacc cttatccgca tgtggacgac
    23161 gtcatcccac tgatggcaga gggcaaaatc ctgccgtatc tggacattcc gttgcagcac
    23221 gccagcccgc gcattctcaa actgatgaag cgtccgggtt ctgtagatcg ccaactggcg
    23281 cgcatcaaac agtggcgcga aatctgcccg gaactgaccc tacgctcaac ctttattgtt
    23341 ggcttccctg gcgagacgga agaagatttc cagatgctac tcgacttcct gaaagaagca
    23401 cgtctggatc gcgttggctg ctttaaatac agcccggttg aaggtgcaga cgccaatgcc
    23461 ctgcctgacc aggttccgga agaagtgaaa gaagaacgct ggaaccgttt catgcaactg
    23521 cagcagcaga tttccgccga gcgcctgcaa gagaaagtgg gtcgtgaaat tctggtgatt
    23581 atcgacgaag tggacgaaga aggcgcgatt ggtcgcagca tggcagatgc accggaaatc
    23641 gacggcgcgg tttatctcaa tggtgaaacc aacgttaagc cgggtgatat cctgcgagtg
    23701 aaagtcgagc acgccgatga gtacgatttg tggggtagcc gggtttaatt aattcaggcc
    23761 gggcaaaatc atttgtccgg cctgcgaata atgattactg ccagctaatc tgcgccctgc
    23821 cgtttttctt actcttatac agccgctcat ccgccagcat tttcaagcgt tcgacggtag
    23881 tacattcatt tgaatgcgct acaccgatac tggtgcttaa attaatgctt cccacctgga
    23941 aatgaacgat ttcttttaga atcgatagca actcttccag cgtgccgcca gtataaatca
    24001 ccgcaaattc gtcacctccg aagcggtaga ctttaccttt atctaccggg atctgtgatt
    24061 taaggatttt caccactttc atcaacactt catcgcccag caaatgaccg aaggtgtcgt
    24121 taatgttttt gaatttatcg acgtcaaaca ggcacaaata accgcctttt tccggcagcg
    24181 cttttagatc ttcgtcaaag gattttctgc ccatcgcttt agtcaggtcg tccaggaatg
    24241 ccgagttata catactgtag agttttttga catcggcagc cgaaaagaaa agcccgccat
    24301 ccagtacgcg ttcaaaatgg ttattaataa tggcttcttt ctccacaata ccacgattaa
    24361 tcactctgcg taccagatag aggcaactgg cacccgatat cactgccacc aatgcggcaa
    24421 agaaaataat caatgtacat acctgaaaat aaacgtcttt tttataagca aatacgtaca
    24481 atccggtcgg caaataggag aagtgctcca ttgcttccga aacggtgaag atggcgtttt
    24541 tagcgttata ttcatcaccg gtcaccgatt taccgttctt gaacagggag aacatcaggt
    24601 tctcgctatc catcgccagc gattttgaca gtaaagaaaa acgttccaga ccaagcaaaa
    24661 atatcagact acgattgtga gttaccggat agaggtaata gacgaaggtt ttaccatcaa
    24721 aagagataaa ccctgtattt ttgacatgtc gatacttaac aacctgcata atatctgcac
    24781 aataattttt ctcaaactcc tttacctgaa tacattcgtc actggaaatc agataaggac
    24841 cgacatcgta caaataagag ggtgaaaccg agtcggcgta agctgtgcgt aagccaacca
    24901 acagttgttc ctccgtagga tttagcgtgt tgatcagatc aattgccaca ctccgccgat
    24961 attcagcaat atttgccacc gcaaaagcag tatcaataac ggactgtttc aggccataca
    25021 cccgttcctt taccatttga gtgtagatcc cgcaggcaac tgctgaaatc atgataaaaa
    25081 tcagcagact gactgtaagt acgaatttat tgattctgga catacgtaaa ctactctttt
    25141 actaattttc cacttttatc ccaggcggag aatggcatgg gtttctggta cagatacccc
    25201 tgagcctgaa cacatcctaa tttgattaat tcacgcgcaa cgtcttcgct ctctacgcct
    25261 tccgcaatca ccgggatccg gttatagcgt gccagatcga aaataatgcg cacaattttt
    25321 cccgcagtac cgaaaatatc gccggtaaat tgcgaatcca cctttatact gtctggatta
    25381 atttcataaa aacgtttcag gtttgacaaa ccggtgccaa agtcatcaat cgatacccgt
    25441 accccttgtt tacgcagttg ttcaatattg agatagaaat gttcattaat tcgctcgatc
    25501 acggtttcag tgatctcaac acaaaggctg ttcgtcttca ggccattttc tttcatgatg
    25561 gtagtaaaac gctcatgaaa gtgtggttcg tttaactgcc agggcgaaac attaatatgc
    25621 agtaagaaat catccttcag cgacgcacgc tgactaaaat gtctgaattc acccatcgcg
    25681 gttttaataa tttgatagcc cagctcattg ataaacccga tatcttcagc aatggtaatg
    25741 aacttcagcg gtgaaataat cccccgctcc gcagacaccc aacggcacaa cgcctccgcg
    25801 cccaaaatgg tgcgggtgtt aatatcaaca atcggttgat agaccaggaa tagttcgcca
    25861 atttccattg catggcgaat attacgcgca atccagatat tctcttctat ttcgttgagt
    25921 ttcgcatcat cacagaaaat aaactctgag aacatatggt tgcggatact ggaaatcgcc
    25981 agtttcagct tttctgcgta actccaggcg ttttccccct gcaaaggttc acaaatagca
    26041 gacttatgga tgcgcagatt gcggtagcca aaactgctgt caaaatcgtt taccgtcgct
    26101 atcttacggg taaacgtctg tacattttcc cggcacagga gtcaatagag atcggcatta
    26161 aatgtcacga tatcgcagca ttgcgcatat ttctcggcaa ttcgctgaca caatttcctg
    26221 acgattttat tgcgttcatc aacaccaaac accgcttcca cagcttccag accacacagt
    26281 ttgatgatcc ccacagccag atactgatta ttgttggact caataatcgc catattttta
    26341 ataaaaccgc tttcctgatc gtaagagatc agcgagagca atttatttgc ccgttcaatt
    26401 tcagcatctt tcatttccgc gatcgaattt ttgatgctga taatctcttt cgcatcgcct
    26461 tcacgaatct tcagggcctc gatttgatcc gtagagtcag gcaaaaacgc cagcgtattg
    26521 accaaatctg tcatttcaga aaattgccga cggaaaactc gagtggtcat taaataaacc
    26581 accagcaata cgataataat gaaagcaaac ggcagataaa aaagtttatc tgatgcgttc
    26641 aataaattac caccaggata ataaaaatag atttcgaata atgcgttgtt tggcaccgct
    26701 gatgtttttt caatatattc cg
//
LOCUS       DAHHTG010000061        26715 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.061, whole genome shotgun sequence.
ACCESSION   DAHHTG010000061 DAHHTG010000000
VERSION     DAHHTG010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26715)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26715)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26715
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.061"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            356..736
                     /locus_tag="NBI74_002490"
     CDS             356..736
                     /locus_tag="NBI74_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001178761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1932811.1"
                     /translation="MQNELKLPNLPEINEDESIRQILNYSTQNNLLFVQSEHDGKIHI
                     LSFTVGLDGKANPKAMDCYVENQGIFSEDKIIALKYAKPTENEMNIISVEAKIVAELR
                     YEYALNLLGRLGVSKSRVGLDFIN"
     gene            981..1853
                     /locus_tag="NBI74_002491"
     CDS             981..1853
                     /locus_tag="NBI74_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009652363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HCD1932812.1"
                     /translation="MTSLETLQSAISNVSVWRQGDVCAPHKPLLLLYVLSQYKAGHPR
                     LFNYGLEIHEPLTRLLKEFGPKRRTDYPNMPFWRLRTDGFWEIANAEGCKPRRGNTQP
                     TKKELIDNQVAGGFDETAYQQLLAHPEVIDQLAQQILMDRFPESIQRILANQLGLDFI
                     DRSKSRDPRFRDIVLRAYHSRCAFCGYDLRLDGALVGIEAAHIHWKTYGGPCVVNNGL
                     ALCSLHHDAFDMGAFGLDENLTIRISGGVSRSPVVDNLFWQRNGQQLHLPHDKSLWPT
                     EQYVGWHRKQIFKA"
     gene            2221..2514
                     /locus_tag="NBI74_002492"
     CDS             2221..2514
                     /locus_tag="NBI74_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001143292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1932813.1"
                     /translation="MPTSSYPEAKSLSLSVSTEAWEKVVSFPPDPSQEDDRLENLIVA
                     TMLTFKSAGPDRKSINFGLYCLPSDGSSNVPLMTPLRLTLEDNHLLVTALHTS"
     gene            2600..3511
                     /locus_tag="NBI74_002493"
     CDS             2600..3511
                     /locus_tag="NBI74_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001748766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1738 domain-containing protein"
                     /protein_id="HCD1932814.1"
                     /translation="MNRSNDLYQKVTDEIIAALEKGVLPWVRPWREGEPVVPMNALSG
                     RFYHGINIPLLWNSAERQGYENDRWLTFTQIRNAGGNIHKGERSTLAVFYLPQQREVV
                     DSNGNTVLDADGNPKVMSYAVVREFRLFNIQQCEGLPEAFSQPVVMVDDPIAAAEQVT
                     RQSAVMITHRRQNRAYYSPGRDCIIMPHPEQFTSREDYYGTLLHELTHATGHASRLNR
                     DGITAGKHTFGDPTYSFEELVAEMGAAFLCAHVGIQSKLQHDSYIASWLKVLQQDKKA
                     IFRASGLARNACEYLLERAQQPLALSA"
     gene            3814..5433
                     /locus_tag="NBI74_002494"
     CDS             3814..5433
                     /locus_tag="NBI74_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003830239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase/relaxase domain-containing protein"
                     /protein_id="HCD1932815.1"
                     /translation="MRIWLTRKKQNTTPSTGEPDVTEGWLHPESAQTLLAETTRQQLI
                     QFIRQSTALPASLFERFWLIPIHRFAELAQLFPASENHHHSHAGGLLDHSLEAACYAA
                     RLRQSYLFPPDAAPEDQAAQSERWTTVIIYAALLHDLGKLITDIEVENFSGKRWFPWH
                     GPLTQPYRFRYLPQRDYLRHPAAAVLLLTQLIPPESLDWLAADTAALSTLLHCLNGQY
                     QYAGLAGELVQKADRASVAFNLGGDPVKAIHRPQTSLPQQIITSLRDLLAHEFRLNNP
                     NGGSDGWLTEEALWLVSKNAADAVRGWLLCQGLDAVPQHNMRLFDEMQAHQLLIPCED
                     KAIWHCRIEASGGWSSELTLLRFSPSLIWEDPQQRPATFIGTVTPIAEIRNETEAVIA
                     TNIEVEQPSPLPEQNETALSGESFWQWLVRNVQAQHLEVNEPNARIHTVAGSVFLVTP
                     GIFKLWLSTCGQNVPEEYWREVQKKFQNLNLHRKQKNGLNIWHCAVVGPRRCSRLKGY
                     LLDDPTPLFGDDVPFNNPHLLLSEGEDTHDL"
     gene            5423..6349
                     /locus_tag="NBI74_002495"
     CDS             5423..6349
                     /locus_tag="NBI74_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000142418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCD1932816.1"
                     /translation="MTFEQLLEEYFFARLLRPDTQSCYCTAVNQFTHWRNVLPAEVTP
                     HMVLEWRHYLLNVRCIKPVSWNHYMRHMRALYNFAIEQGLLEQFINPFQKTSLRESRK
                     KKKTLTREQILASRKVLNQFIEREKMQRGYRSPLYPAWFWLTVVETFNYTAIRLNQLI
                     HLRVRDIDLVHDTLFIQIEGSKSHDEHIVSIASRLRPYLEHLLEEAKTKGIRADDQLF
                     NINRFSRRTLRQGKPMTENQVSYFFAKLSDACHSRFPSHRYRHTVATELMQKPEQNLY
                     VTQKLLGHRDIKVTLSYIEHNVEMLRSCVERD"
     gene            complement(6499..6583)
                     /locus_tag="NBI74_002496"
     tRNA            complement(6499..6583)
                     /locus_tag="NBI74_002496"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6547..6549),aa:Leu,seq:caa)
     gene            6779..7798
                     /gene="ahr"
                     /locus_tag="NBI74_002497"
     CDS             6779..7798
                     /gene="ahr"
                     /locus_tag="NBI74_002497"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent aldehyde reductase Ahr"
                     /protein_id="HCD1932817.1"
                     /translation="MSMIKSYAAKEAGGELEVYEYDPGELKPQDIEVQVDYCGICHSD
                     LSMIDNEWGFSQYPLVAGHEVIGRVVALGSAAQDKGLQVGQRVGIGWTARSCGHCDAC
                     ISGNQINCEQGAVPTIMNRGGFAEKLRADWQWVIPLPENIDIESAGPLLCGGITVFKP
                     LLMHHITATSRVGVIGIGGLGHIAIKLLHAMGCEVTAFSSNPAKEQEVLAMGADKVVN
                     SRDPQALKTLAGQFDLIINTVNVSLDWQPYFEALTYGGNFHTVGAVLTPLSVPAFTLI
                     AGDRSVSGSATGTPYELRKLMRFAARSKVAPTTELFPMSKINDAIQHVRDGKARYRVV
                     LKADF"
     gene            complement(7802..8365)
                     /gene="idnK"
                     /locus_tag="NBI74_002498"
     CDS             complement(7802..8365)
                     /gene="idnK"
                     /locus_tag="NBI74_002498"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HCD1932818.1"
                     /translation="MAGESFILMGVSGSGKTLIGSKVAALLSAKFIDGDDLHPAKNID
                     KMSQGIPLSDEDRLPWLERLNDASYSLYKKNETGFIVCSSLKKQYRDILRKGSPHVHF
                     LWLDGDYETILARMQRRAGHFMPVALLKSQFEALERPQEDEQDIVRIDINHDIANVTE
                     QCRQAVLAIRQNRICAKEGSASDQRCE"
     gene            8582..9613
                     /gene="idnD"
                     /locus_tag="NBI74_002499"
     CDS             8582..9613
                     /gene="idnD"
                     /locus_tag="NBI74_002499"
                     /EC_number="1.1.1.264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-idonate 5-dehydrogenase"
                     /protein_id="HCD1932819.1"
                     /translation="MQVKTQSCVVAGKKTVAVTEQTIDWNNNGTLVQITRGGICGSDL
                     HYYQEGKVGNFMIKAPMVLGHEVIGKVIHSDSSELHEGQTVAINPSKPCGHCKYCIEH
                     NENQCTDMRFFGSAMYFPHVDGGFTRYKMVETSQCVPYPAKTDEKVMAFAEPLAVAIH
                     AAHQAGELQGKRVFISGVGPIGCLIVSAVKTLGAAEIVCADVSPRSLSLGKEMGADVL
                     VNPQNDDMDHWKAEKGYFDVSFEVSGHPSSVNTCLEVTRARGVMVQVGMGGAMAEFPM
                     MTLIGKEISLKGSFRFTSEFNTAVSWLANGVINPLPLLSAEYPFTDLEEALRFAGDKT
                     QAAKVQLVF"
     gene            9637..10401
                     /gene="idnO"
                     /locus_tag="NBI74_002500"
     CDS             9637..10401
                     /gene="idnO"
                     /locus_tag="NBI74_002500"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HCD1932820.1"
                     /translation="MNDLFSLAGKNILITGSAQGIGFLLATGLGKYGAQIIINDITAE
                     RAELAVEKLHQEGIQAVAAPFNVTHKHEIDAAVEHIEKDIGPIDVLVNNAGIQRRHPF
                     TEFPEQEWNDVIAVNQTAVFLVSQAVTRHMVERKAGKVINICSMQSELGRDTITPYAA
                     SKGAVKMLTRGMCVELARHNIQVNGIAPGYFKTEMTKALVEDEAFTAWLCKRTPAARW
                     GDPQELIGAAVFLSSKASDFVNGHLLFVDGGMLVAV"
     gene            10466..11785
                     /gene="idnT"
                     /locus_tag="NBI74_002501"
     CDS             10466..11785
                     /gene="idnT"
                     /locus_tag="NBI74_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gnt-II system L-idonate transporter"
                     /protein_id="HCD1932821.1"
                     /translation="MPLIIIAAGVALLLILMIGFKVNGFIALVLVAAVVGFAEGMDAQ
                     AVLHSIQNGIGSTLGGLAMILGFGAMLGKLISDTGAAQRIATTLIATFGKKRVQWALV
                     ITGLVVGLAMFFEVGFVLLLPLVFTIVASSGLPLLYVGVPMVAALSVTHCFLPPHPGP
                     TAIATIFEANLGTTLLYGFIITIPTVIVAGPLFSKLLTRFEKAPPEGLFNPHLFSEEE
                     MPSFWNSIFAAVIPVILMAIAAVCEITLPKTNTVRLFFEFVGNPAVALFIAIVIAIFT
                     LGRRNGRTIEQIMDIIGDSIGAIAMIVFIIAGGGAFKQVLVDSGVGHYISHLMTGTTL
                     SPLLMCWTVAALLRIALGSATVAAITTAGVVLPIINVTHADPALMVLATGAGSVIASH
                     VNDPGFWLFKGYFNLTVGETLRTWTVMETLISIMGLLGVLAINAVLH"
     gene            11852..12850
                     /gene="idnR"
                     /locus_tag="NBI74_002502"
                     /gene_synonym="gntH"
                     /gene_synonym="yjgS"
     CDS             11852..12850
                     /gene="idnR"
                     /locus_tag="NBI74_002502"
                     /gene_synonym="gntH"
                     /gene_synonym="yjgS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator IdnR"
                     /protein_id="HCD1932822.1"
                     /translation="MRNHRISLQDIATLAGVTKMTVSRYIRSPKKVAKETGERIAKIM
                     EEINYIPNRAPGMLLNAQSYTLGILIPSFQNQLFADILAGIESVTSEHNYQTLIANYN
                     YDRDSEEESVINLLSYNIDGIILSEKYHTIRTVKFLRSATIPVVELMDVQGERLDMEV
                     GFDNRQAAFDMVCTMLEKRVRHKILYLGSKDDTRDEQRYQGYCDAMMLHNLSPLRMNP
                     RAISSIHLGMQLMRDALSANPDLDGVFCTNDDIAMGALLLCRERNLAVPEQISIAGFH
                     GLEIGRQMIPSLASVITPRFDIGRMAAQMLLSKIKNNDHNHNTVDLGYQIYHGNTL"
     gene            12928..14430
                     /locus_tag="NBI74_002503"
                     /pseudo
     CDS             12928..14430
                     /locus_tag="NBI74_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313267.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF853 domain-containing protein"
     gene            complement(14591..15673)
                     /gene="lptG"
                     /locus_tag="NBI74_002504"
     CDS             complement(14591..15673)
                     /gene="lptG"
                     /locus_tag="NBI74_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313266.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HCD1932823.1"
                     /translation="MQPFGVLDRYIGKTIFTTIMMTLFMLVSLSGIIKFVDQLKKAGQ
                     GSYDALGAGMYTLLSVPKDVQIFFPMAALLGALLGLGMLAQRSELVVMQASGFTRMQV
                     ALSVMKTAIPLVLLTMAIGEWVAPQGEQMARNYRAQAMYGGSLLSTQQGLWAKDGNNF
                     VYIERVKGDEELGGISIYAFNENRRLQSVRYAATAKFDPEHKVWRLSQVDESDLTNSK
                     QITGSQTVSGTWKTNLTPDKLGVVALDPDALSISGLHNYVKYLKSSGQDAGRYQLNMW
                     SKIFQPLSVAVMMLMALSFIFGPLRSVPMGVRVVTGISFGFVFYVLDQIFGPLTLVYG
                     IPPIIGALLPSASFFLISLWLLMRKS"
     gene            complement(15673..16773)
                     /gene="lptF"
                     /locus_tag="NBI74_002505"
     CDS             complement(15673..16773)
                     /gene="lptF"
                     /locus_tag="NBI74_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HCD1932824.1"
                     /translation="MIIIRYLVREALKSQLAILFILLLIFFCQKLVRILGAAVDGDIP
                     ANLVLSLLGLGVPEMAQLILPLSLFLGLLMTLGKLYTESEITVMHACGLSKAVLVKAA
                     MILAVFTAIVAAVNVMWAGPWSSRHQDEVLAEAKANPGMAALAQGQFQQATNGSSVLF
                     IESVDGSDFKDVFLAQIRPKGNARPSVVVADSGHLTQLRDGSQVVTLNQGTRFEGTAL
                     LRDFRITDFQDYQAIIGHQAVALDPNDTDQMDMRTLWNTDTDRARAELNWRITLVFTV
                     FMMALMVVPLSVVNPRQGRVLSMLPAMLLYLLFFLIQTSLKSNGGKGKLDPTLWMWTV
                     NLIYLALAIVLNLWDTVPVRRLRASFSRKGAV"
     gene            17040..18551
                     /gene="pepA"
                     /locus_tag="NBI74_002506"
     CDS             17040..18551
                     /gene="pepA"
                     /locus_tag="NBI74_002506"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HCD1932825.1"
                     /translation="MEFSVKSGSPEKQRSACIVVGVFEPRRLSPIAEQLDKISDGYIS
                     ALLRRGELEGKPGQTLLLHHVPNVLSERILLIGCGKERELDERQYKQVIQKTINTLND
                     TGSMEAVCFLTELHVKGRNNYWKVRQAVETAKETLYSFDQLKTNKSEPRRPLRKMVFN
                     VPTRRELTSGERAIQHGLAIAAGIKAAKDLGNMPPNICNAAYLASQARQLADSYSKNV
                     ITRVIGEQQMKELGMHSYLAVGQGSQNESLMSVIEYKGNASEDARPIVLVGKGLTFDS
                     GGISIKPSEGMDEMKYDMCGAAAVYGVMRMVAELQLPINVIGVLAGCENMPGGRAYRP
                     GDVLTTMSGQTVEVLNTDAEGRLVLCDVLTYVERFEPEAVIDVATLTGACVIALGHHI
                     TGLMANHNPLAHELIAASEQSGDRAWRLPLGDEYQEQLESNFADMANIGGRPGGAITA
                     GCFLSRFTRKYNWAHLDIAGTAWRSGKAKGATGRPVALLAQFLLNRAGFNGEE"
     gene            18905..19348
                     /gene="holC"
                     /locus_tag="NBI74_002507"
     CDS             18905..19348
                     /gene="holC"
                     /locus_tag="NBI74_002507"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004098129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HCD1932826.1"
                     /translation="MKNATFYLLDNDTTVDGLSAVEQLVCEIAAERWRSGKRVLIACE
                     DEKQAYRLDEALWARPAESFVPHNLAGEGPRGGAPVEIAWPQKRSSSPRDILISLRTS
                     FADFATAFTEVVDFVPYEDSLKQLARERYKAYRVAGFNLNTATWK"
     gene            19348..22203
                     /gene="valS"
                     /locus_tag="NBI74_002508"
     CDS             19348..22203
                     /gene="valS"
                     /locus_tag="NBI74_002508"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000416368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HCD1932827.1"
                     /translation="MEKTYNPQDIEQPLYEHWEKQGYFKPNGDESQESFCIMIPPPNV
                     TGSLHMGHAFQQTIMDTMIRYQRMQGKNTLWQVGTDHAGIATQMVVERKIAAEEGKTR
                     HDYGREAFIDKIWEWKAESGGTITRQMRRLGNSVDWERERFTMDEGLSNAVKEVFVRL
                     YKEDLIYRGKRLVNWDPKLRTAISDLEVENRESKGSMWHIRYPLADGAKTADGKDYLV
                     VATTRPETLLGDTGVAVNPEDPRYKDLIGKYVILPLVNRRIPIVGDEHADMEKGTGCV
                     KITPAHDFNDYEVGKRHALPMINILTFDGDIRESAQVFDTKGNESDVYSSEIPAEFQK
                     LERFAARKAVVAAVDALGLLEEIKPHDLTVPYGDRGGVVIEPMLTDQWYVRADVLAKP
                     AVEAVENGDIQFVPKQYENMYFSWMRDIQDWCISRQLWWGHRIPAWYDEAGNVYVGRN
                     EDEVRKENNLGADVALRQDEDVLDTWFSSALWTFSTLGWPENTDALRQFHPTSVMVSG
                     FDIIFFWIARMIMMTMHFIKDENGKPQVPFHTVYMTGLIRDDEGQKMSKSKGNVIDPL
                     DMVDGISLPELLEKRTGNMMQPQLADKIRKRTEKQFPNGIEPHGTDALRFTLAALAST
                     GRDINWDMKRLEGYRNFCNKLWNASRFVLMNTEGQDCGFNGGEMTLSLADRWILAEFN
                     QTIKAYREALDSFRFDIAAGILYEFTWNQFCDWYLELTKPVMNGGTEAELRGTRHTLV
                     TVLEGLLRLAHPIIPFITETIWQRVKVLCGITADTIMLQPFPQYDASQVDEAALADTE
                     WLKQAIVAVRNIRAEMNIAPGKPLELLLRGCSADAERRVNENRGFLQTLARLESITVL
                     PADDKGPVSVTKIVDGAELLIPMAGLINKEDELARLAKEVAKIEGEISRIENKLANEG
                     FVARAPEAVIAKEREKLEGYAEAKAKLIEQQAVIAAL"
     gene            complement(22250..23437)
                     /locus_tag="NBI74_002509"
     CDS             complement(22250..23437)
                     /locus_tag="NBI74_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001622551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF898 family protein"
                     /protein_id="HCD1932828.1"
                     /translation="MNNVNIGKDNSRHSFVFTGKGGEYFLICLVNFLLTIITLGIYGP
                     WALIKCRRYIYQHVTLKGQPFSYKGTGGAIFVSMLLIVVVYLLSISCFAGQHFALGLF
                     LFALLICGIPCMAVKSLQYQANMTSLNDIRFGFNCSMMRAWWVMLGLPVLLALVFWFA
                     LYLIAQVTTSIGGLFFNLVALSLLSAIGLGVVHGITYSKWMPLLGNNATFGIHKFSIQ
                     VNVKECIKGCMLAILTMVPFIIVIGIMIAPLFQQLMMMTMLGRSDAGSEFVLQYYPQI
                     MASYFLYFVAILVFASYLYVTLRSLFLNNLTLANGAIRFHSSVTAIGMLLRMLALLMG
                     ASITCGLAYPWLKMWMVSWIANNTHVQGDLDSLELTNDDKPQDSGSLMWISRGIMPYV
                     PFI"
     gene            23630..24133
                     /locus_tag="NBI74_002510"
     CDS             23630..24133
                     /locus_tag="NBI74_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCD1932829.1"
                     /translation="MNVVASPALRLRKLTVADNPAIAHVIRQVSAEYGLTADKGYTVA
                     DPNLDELYQVYSQPGHAYWVVEYEGEVVGGGGIAPLTGSESDICELQKMYFLPAIRGK
                     GLAKKLALMAMEQAREMGFKRCYLETTAFLKEAIALYEHLGFEHIDYALGCTGHVDCE
                     VRMLRKL"
     gene            complement(24310..24726)
                     /gene="rraB"
                     /locus_tag="NBI74_002511"
     CDS             complement(24310..24726)
                     /gene="rraB"
                     /locus_tag="NBI74_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E inhibitor RraB"
                     /protein_id="HCD1932830.1"
                     /translation="MANPEQLEEQREETRLIIEELLEDGSDPDALYTIEHHLSADDLE
                     TLEKAAVEAFKLGYEVTDPEELEVEDGDIVICCDILSECALNADLIDAQVEQLMTLAE
                     KFDVEYDGWGTYFEDPNGEDGDDEDFVDEDDDGIRH"
     gene            24888..25892
                     /gene="argF"
                     /locus_tag="NBI74_002512"
     CDS             24888..25892
                     /gene="argF"
                     /locus_tag="NBI74_002512"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HCD1932831.1"
                     /translation="MSGFYHKHFLKLLDFTPAELNSLLQLAAKLKADKKSGKEEARLT
                     GKNIALIFEKDSTRTRCSFEVAAYDQGARVTYLGPSGSQIGHKESIKDTARVLGRMYD
                     GIQYRGYGQEIVETLAEYAGVPVWNGLTNEFHPTQLLADLLTMQEHLPGKAFNEMTLV
                     YAGDARNNMGNSMLEAASLTGLDLRLVAPQACWPEAALVAECSALAQKHGGKITLTED
                     IASGVKGADFIYTDVWVSMGEPKEKWAERIALLRDYQVNSKMMALTGNSQVKFLHCLP
                     AFHDEQTTLGKKMAAEFGLYGGMEVTDEVFESPASIVFDQAENRMHTIKAVMVATLAK
                     "
     gene            complement(25937..26389)
                     /locus_tag="NBI74_002513"
     CDS             complement(25937..26389)
                     /locus_tag="NBI74_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HCD1932832.1"
                     /translation="MIVGNIHHLQSWLPEELREAIEYIKSHVSDETAKGKHAIDGDRL
                     FYLISEDTTEPGELRRAEYHARYLDIQIVLKGQEGMTFSTQPAGVPETDWLADKDIAF
                     IGQGIDEKTVILNEGDFVVFYPGEVHKPLCAVGAPAQVRKAVVKLLKA"
BASE COUNT         6791 a         6761 c         6719 g         6444 t
ORIGIN      
        1 taaattgtgg gaagaattag catacgaaat aaaacagagt cttgatgatt ttacaattaa
       61 tcttgtccgc gacttaaaga aaattaactc acctttaaac tatttcatag agaaatctac
      121 acttgatgct gaacttgaac aaattaaaca tgcaaattgt ttcagttgtt caaaaaaaat
      181 aactgctcat catttggtta cggggcatgt tttggagttc aataataatc actggttgtg
      241 tctaactcct atgtgtgacc ttgttcctgg tcagaaaaac ggaaatagtt tactccctgt
      301 tacgctcgtg aaaatgtatg atgcgaaagt tgctttaaat aatacacgta aaaatatgca
      361 aaacgagctt aaactaccca atttgccaga aatcaacgaa gatgaatcaa ttagacaaat
      421 actaaattat tccacacaga ataatctatt gttcgttcag tctgaacatg acgggaaaat
      481 acatattctt agtttcaccg ttggactcga tggcaaggca aatcctaaag caatggattg
      541 ctatgtggaa aatcaaggta ttttctctga agataaaata atagcactaa aatatgccaa
      601 gcccactgaa aatgaaatga acataatatc cgtagaagca aaaatagttg ctgaattacg
      661 ctacgaatat gctttgaatt tattaggtag actcggtgta tcaaaatctc gagtcggatt
      721 agattttatc aactaaggtg cgttagcacg cacctagtct gacaggtacc agttgtttat
      781 ataggtatct gtcagactac atcctcttta ggtttctctc gcccagataa ttttttccat
      841 caagtgacat tttcattgat gtctaactct cagacattaa agtgtctaac ttccttatta
      901 atgtcacaag caacaattga atttcaccgc ttttgcgagc atgatcgcaa taatatcagc
      961 ccgttacccg gttaattcct atgacatcac tcgaaacact gcaatcggct atctctaacg
     1021 tctctgtatg gcgtcagggt gatgtatgcg cgccgcataa accgttgctg ctgctgtatg
     1081 tgttgtcaca gtacaaagca ggccacccgc gcctgtttaa ctacggccta gagatccacg
     1141 aaccactcac tcgcctgcta aaagagtttg gccccaagcg acgcactgac tatcccaata
     1201 tgcctttctg gcgactcaga actgacggct tctgggaaat tgctaatgcg gaaggctgca
     1261 aaccccgtag aggcaacacc cagccgacaa agaaagagct gattgataat caggtagcgg
     1321 ggggttttga tgaaacagct taccagcaac tgcttgcaca ccctgaagta attgaccaac
     1381 tggcccagca gatcctgatg gatcgtttcc ccgagagtat tcagcggatc ctcgccaacc
     1441 aactgggtct ggattttatc gaccgttcaa agagccgcga tccgcgtttc agggatatcg
     1501 tgcttcgggc ttaccattcg cgatgtgctt tctgcggtta cgatctacga ctcgatggtg
     1561 cgctggttgg tattgaagcc gcccatattc actggaaaac ctatggcggg ccgtgtgtgg
     1621 taaacaacgg tctggcgcta tgttcgctgc accacgatgc ttttgatatg ggcgcattcg
     1681 ggctggatga aaaccttacc atccgcatct ccggcggcgt cagccgtagc ccggtggtgg
     1741 ataacctgtt ctggcaacgg aacggccagc agttacacct tcctcacgac aaatcgctgt
     1801 ggcccactga acaatacgtc ggctggcatc gtaaacagat cttcaaagcc tgagaccgtg
     1861 agcttcgcag gtatcatcga ttgcccaaac tgctttatcc cctacaacgg ataaattgct
     1921 tttaacccct atagcggata aatccagcac accagtgttg gacttcagaa taacgaatcc
     1981 aaactctagc cctgagacac caggctcttg attattattg ataccgtatt aatctgtacg
     2041 aagtttgacc cgcaaccaga gccttcaggt tgccacccta cccggtgagg ctcgcctttt
     2101 cgtccgctcc gtatcatcct cgcggacagc gcttagcgcc tgatacatcc tctaccccac
     2161 catgcaggaa tccctccctg ccgggcggtg tttctctgct ttcaccattg gagaaacacg
     2221 atgccaactt catcttaccc tgaagcaaaa tccttatcgc tgtctgtctc aactgaggcc
     2281 tgggaaaagg tagttagttt tccacccgat ccctctcagg aagacgaccg tctcgaaaac
     2341 ctgattgtcg cgaccatgct gacattcaaa agcgcaggcc cggaccgtaa gagtatcaat
     2401 ttcggtctgt actgtttgcc ctctgacggc agcagcaatg taccgctgat gacgccgctg
     2461 cgcttaacgc ttgaagataa ccacctgttg gttactgccc ttcacaccag ctgattacct
     2521 cagcatcctt tactcctcgt gccgggcgac ccccgaccgg gagcgctgta cccggcttct
     2581 ttatcatgag gttacgctta tgaacagaag caacgatctt taccagaaag tcacagatga
     2641 aattatcgcc gcgctggaga aaggcgtgct cccctgggtt cgcccgtggc gggaagggga
     2701 accggtggtg ccgatgaatg ccctgtccgg gcggttttat cacggcatca atattcccct
     2761 gctctggaac agcgcagaac gacagggata tgaaaacgat cgctggctga ccttcactca
     2821 aattcgcaac gcgggcggta acatccacaa aggcgaaaga tctacactgg ccgtgtttta
     2881 tctgccccag cagcgcgaag ttgtggacag caacggaaat acggttcttg atgctgacgg
     2941 caaccctaaa gtgatgtctt acgctgtagt acgcgagttc cggctgttca acattcaaca
     3001 atgtgaaggg ctgccggagg cgttttcaca gcctgtcgtg atggtcgatg atcctatcgc
     3061 cgccgctgag caggttaccc ggcaaagtgc ggtgatgatc actcaccgac gccagaaccg
     3121 ggcgtattac tcgccggggc gcgactgcat catcatgccg catcctgagc agttcacttc
     3181 gcgtgaggat tattacggca cgctgctgca tgaactgacg cacgctaccg gacacgcttc
     3241 acgattgaac cgggacggca tcactgccgg aaagcatacc ttcggcgatc cgacgtacag
     3301 ttttgaagag ctggtcgccg aaatgggagc ggctttcctg tgtgctcacg ttggcatcca
     3361 gtcgaagcta cagcatgaca gttacatcgc atcgtggctg aaggtgttgc agcaagacaa
     3421 aaaagcgatt ttccgcgcca gcggtttagc gcgtaatgcc tgtgagtatc tgcttgaacg
     3481 ggcgcagcag ccgttagcgc tgagcgcatg accttcatat caacggcgga gcatcctgct
     3541 ctgccgtttt tctccactcc aataaatttg cgcaacccaa taaacagttt tgccccaaat
     3601 ctgacggcac caaaaggcaa aatccaaagg gttaagggga agagagtgaa gggggaacgg
     3661 gcttacccga aaaggggctt cccttcgtct ttcgagttgc aggtgcgttg gctttcctcg
     3721 ctcaccccag ttacttactt atgtaagctc caggggattc actgcgtcac cgccttcctg
     3781 cacctcaaaa tccattggga ataaagggcc attatgcgga tatggctgac gcgcaaaaag
     3841 caaaacacca caccatccac tggcgaacca gacgtcacgg agggatggct gcatcctgaa
     3901 tccgcgcaaa cgctactagc tgaaactacc cgccaacagc tgatccagtt tattcgccag
     3961 agcaccgccc ttcccgcctc actgtttgaa cgcttctggc tgataccaat ccaccgcttc
     4021 gcagaactgg cgcagttgtt cccagcctcg gaaaaccacc accattccca cgcaggtggc
     4081 ctgctcgatc acagcctcga agccgcctgc tatgccgcac ggctgcggca gagttatctt
     4141 tttccgcctg atgcagcgcc ggaagatcag gccgcgcagt ccgaacgctg gaccaccgtt
     4201 atcatctacg ccgcgctgct gcacgatctg ggtaaactca tcaccgatat tgaagtggaa
     4261 aattttagcg ggaaacgctg gtttccatgg catggcccgc tgacccaacc ctatcgattt
     4321 cgctatctgc cgcaacgtga ttacctgcgc catccggctg cggcagttct cctgcttacc
     4381 caactgatcc ctccggaaag cctcgactgg ctcgccgccg acaccgctgc attatccacc
     4441 ctactccact gcctgaacgg acagtatcag tatgccgggc tggcgggtga actggtgcag
     4501 aaagccgacc gggcgtcggt ggcatttaat ctcggcggcg atccggtcaa ggcgatccat
     4561 cgtccgcaaa cctcgctgcc gcagcaaatt atcaccagtt tgcgcgacct gctggcgcat
     4621 gagtttcggc tcaacaaccc gaatggaggc tctgacggct ggctgacgga agaggcgctg
     4681 tggctggtca gcaagaacgc tgccgatgct gtgcgcggct ggttgctgtg ccaggggctt
     4741 gatgctgtac cgcagcataa catgcggctg ttcgacgaga tgcaggcaca ccagttactc
     4801 atcccctgtg aggacaaagc catctggcat tgccggattg aggccagcgg cggttggagc
     4861 agtgaactca cgctgttacg cttttcccct tcgctgattt gggaagatcc acaacagcgg
     4921 cccgctacct tcattggaac cgtcacaccg atagcggaaa tccgaaatga gacagaagcg
     4981 gtgattgcaa cgaacattga agtagaacaa ccatcccctc tacccgaaca aaacgaaacc
     5041 gcactatccg gcgaatcctt ctggcagtgg ctggtgcgta acgttcaggc tcaacatctg
     5101 gaagtcaatg aacctaacgc ccgaatccat accgtcgccg ggtcggtgtt tctggtgacg
     5161 cccggtattt tcaaactctg gcttagcacc tgcggtcaga atgtcccaga agaatactgg
     5221 cgggaagtac agaagaagtt ccagaacctg aatcttcacc gaaaacaaaa aaacggcctc
     5281 aacatctggc actgcgccgt tgtcggccca cgccgttgtt cacgtctgaa gggatatctg
     5341 cttgacgatc ccacgccgct ttttggagat gacgttccct ttaataaccc tcatctcttg
     5401 ctcagtgaag gagaagacac tcatgacctt tgaacagctc ctggaagagt atttttttgc
     5461 ccgtctgctg cgccctgata cccagagctg ctattgcacg gcggtaaatc agtttaccca
     5521 ctggcgcaac gtcctacccg ccgaagtcac accgcatatg gtactggagt ggcgccatta
     5581 cctgctaaat gtgcgctgta tcaagccggt gagctggaat cactatatgc gccatatgcg
     5641 agccttgtat aacttcgcca ttgaacaggg gctactggag cagttcatca atccattcca
     5701 gaaaacatcg ctgcgggaat ctcgtaagaa aaagaaaacc ctgacccgcg agcaaatcct
     5761 cgcatcccgc aaagtgctga accagtttat cgaacgggaa aaaatgcagc gtggctatcg
     5821 ttcgccttta tatccggcat ggttttggct gaccgtagtc gaaaccttca actacaccgc
     5881 tatccggctg aatcagctta ttcacctgcg ggtcagggat atcgatctgg tccatgacac
     5941 gctgtttatt cagatcgaag gcagtaaaag ccatgatgaa catattgttt ccatcgcctc
     6001 ccgactgcgg ccctatctgg agcatctgct tgaagaagcg aaaacaaagg gaatacgggc
     6061 tgacgatcag ttgttcaaca tcaatcgttt cagtcggcgg acgctacgcc agggtaaacc
     6121 gatgacagaa aatcaggtga gttatttctt tgccaaactc agcgatgcct gccacagccg
     6181 ttttccatca catcgctacc gtcataccgt tgccacagaa ttgatgcaga aaccggagca
     6241 aaatttgtat gtcacgcaga aactactcgg acaccgcgat attaaggtga cgctcagcta
     6301 tattgaacac aatgtcgaga tgctcagaag ttgcgtggag agagattgac aaaacaggga
     6361 gtgagtgaaa agatccggcc cgaggcttta taaacaaaaa aagccggaga tgcggccatt
     6421 ttgtagttgc cgtcaacgtc cagcttcatt gtacaacgta ttgattgttg taggttttac
     6481 gattatcgca atcgtttttg gtgccgaagg ccggactcga accggcacgt atttctacgg
     6541 ttgattttga atcaactgcg tctaccgatt tcgccacttc ggcactgaag aggtatgcgg
     6601 aaaacgttgt gaattatacc tgtcacgcac caccatgcaa gcgccagctt gctcgcacct
     6661 cgctaagtgc tgaaattttc agcattgcat accgcgatgt gtaacctttg tcacactcca
     6721 ggcaccccgc cctgccatgc tctacacttc ccagacaaca ccagagaagg accaaaaaat
     6781 gtcgatgata aaaagctatg ccgcaaaaga agcgggcggc gaactggaag tttatgagta
     6841 cgatcccggt gagctgaagc cacaagatat tgaagtgcag gtggattact gcgggatctg
     6901 ccattccgat ctgtcgatga tcgataacga atggggattt tcacagtatc cgctggttgc
     6961 cgggcatgag gtgattgggc gcgtggtggc actcgggagc gccgcgcagg ataaaggttt
     7021 gcaggtcggt cagcgtgtcg ggattggctg gacggcgcgt agctgtggtc actgcgacgc
     7081 ctgtattagc ggtaatcaga tcaactgcga gcaaggtgcg gtgccgacga ttatgaatcg
     7141 cggtggcttt gccgagaagt tgcgtgcgga ctggcaatgg gtgattccac tgccagaaaa
     7201 tattgatatc gagtccgccg ggccgctgtt gtgcggcggt atcacggtct ttaaaccact
     7261 gttgatgcac catatcactg ctaccagccg cgttggggta attggtattg gcgggctggg
     7321 gcatatcgct ataaaacttc tgcacgcaat gggatgcgag gtgacagcct ttagttcaaa
     7381 tccggcgaaa gagcaggaag tactggcgat gggtgccgat aaagtggtga atagccgcga
     7441 tccgcaggca ctgaaaactc tggcggggca gtttgatctc attatcaata ctgtgaacgt
     7501 cagcctcgac tggcagcctt attttgaggc gctgacgtat ggcggtaatt tccatacggt
     7561 cggtgcggtt ctcacgccgc tgtctgttcc ggcctttacg ttaattgcgg gcgatcgcag
     7621 tgtctctggc tctgctactg gcacgcctta tgagttgcgt aagctgatgc gttttgccgc
     7681 ccgcagcaag gttgcgccga ctaccgaatt gttcccgatg tcgaaaatta acgacgccat
     7741 ccagcatgtg cgcgacggta aggcgcgtta ccgcgtggtg ttgaaagccg atttttgaaa
     7801 atcattcgca gcgctgatct gaggcgctgc cctctttcgc acatattctg ttttgtcgta
     7861 tcgccagcac agcctgccga cattgttcag tgacattggc aatatcatgg ttgatatcaa
     7921 tgcgcacaat atcctgttca tcttcttgtg gacgctccag tgcctcaaac tgacttttta
     7981 gtaacgctac cggcataaaa tgcccagccc gacgctgcat tcgcgcgaga atagtttcat
     8041 agtcgccatc taaccagagg aaatgaacat gggggctacc cttgcgtaaa atatcacgat
     8101 actgtttttt taatgatgaa cagacaataa atcctgtttc attcttttta taaagactgt
     8161 atgaagcatc atttaagcgt tccagccagg gaagtcgatc ttcatcagat aatggaatac
     8221 cctgcgacat tttatctata tttttggctg gatgaagatc gtcaccatca ataaatttag
     8281 cagataataa cgcggcaacc ttgctaccaa ttaatgtttt accactccct gaaacgccca
     8341 tcaaaataaa gctttcaccc gccatttaaa atctcctgaa tcatcttacc tgctgcgata
     8401 ctacgcctgg tggcataata atttcacgtt tttttatttc tgctgataag aattacaagg
     8461 cacatcacgt tatgcgtaac atagtaatgt aacaattttc tgacgtgatc ttcatcacaa
     8521 ataataacaa ttaaaaccgc ttaaatgctt ccgaggtgtc tacctgacca gtgaggaatt
     8581 tatgcaagtg aaaacacagt cctgcgttgt tgcgggcaag aaaactgttg ccgttaccga
     8641 gcagacgata gattggaata ataatggaac attagtacaa ataacccgag gtggaatttg
     8701 cggttccgat ttacattatt atcaggaagg aaaagtaggt aatttcatga taaaggcacc
     8761 gatggtgtta ggtcatgaag ttatcggtaa agttattcat agcgactcat cagaattaca
     8821 tgaagggcaa acggtagcca ttaatccgtc taaaccgtgc ggtcactgca aatactgcat
     8881 tgaacataac gagaatcagt gtacagatat gcgttttttt ggcagtgcca tgtatttccc
     8941 tcatgttgat ggtggtttta cccgttataa aatggtcgaa acgtcgcaat gtgtccctta
     9001 tccggccaaa actgacgaaa aggttatggc ttttgccgaa cctttagccg tcgcgattca
     9061 tgccgcacat caggccggcg agttacaggg caagcgagta tttatttctg gtgttgggcc
     9121 cattggctgc ctgattgtca gtgctgtgaa aacactgggg gccgcggaaa ttgtctgtgc
     9181 cgatgtgagt ccccgttccc tttcgctggg taaagagatg ggggcggatg tgctcgtaaa
     9241 cccacaaaac gacgacatgg atcactggaa agcggaaaaa ggctatttcg atgtcagctt
     9301 tgaagtgtcc ggtcatcctt catcagtgaa tacctgtctg gaggtcactc gtgcacgcgg
     9361 cgtaatggtg caggtaggta tgggaggcgc gatggcagaa ttcccaatga tgacattgat
     9421 tggtaaggag atttcactca aaggctcttt ccgttttacc agcgaattta ataccgcagt
     9481 gtcatggctg gcgaatggcg ttatcaatcc actgccttta ctgagtgctg aatatccctt
     9541 cactgacctg gaagaggcgc tacgtttcgc cggtgataaa acccaggcag caaaagtcca
     9601 gcttgttttc taagagataa ataaaggaat aatacaatga acgatctatt ttcactggca
     9661 ggaaaaaata tcttgattac cggttcagca cagggcattg gctttttact ggcaaccggc
     9721 ctgggtaaat atggcgcaca aataattatt aatgatatta ctgccgaacg cgcagaactt
     9781 gctgtagaaa aactccacca ggagggtatt caggccgttg ccgcaccttt taatgttact
     9841 cataaacatg aaattgatgc cgccgttgaa catatcgaaa aggacatcgg ccccattgat
     9901 gtgctggtga ataacgccgg tatccagcgc cgtcatcctt ttactgagtt ccctgaacaa
     9961 gagtggaatg atgtgatcgc agtaaaccag accgccgtgt tcctggtatc gcaagcagta
    10021 actcgtcaca tggttgaacg caaggcaggt aaagttatta atatttgctc gatgcaaagc
    10081 gaactgggac gtgacaccat caccccttat gccgcatcga aaggggcggt aaaaatgctc
    10141 acccgcggca tgtgcgtcga gctggcgcgc cacaatattc aggtcaacgg tattgcgccg
    10201 ggctatttca aaacagaaat gactaaagca ctggttgagg acgaagcctt caccgcctgg
    10261 ttgtgcaaac ggacccccgc cgcacgctgg ggagatccgc aggaactgat tggtgctgcg
    10321 gtgttccttt cttcaaaagc ctctgatttc gtaaacggcc acctgttgtt tgttgatggc
    10381 ggcatgttag tggctgttta acacctacta ccctacgtgc agcgcgaaag cgctgctttt
    10441 ctggcactaa taagagataa agactatgcc attaatcatt attgcggcag gcgtcgcgct
    10501 gcttcttatc ctgatgatcg gctttaaagt taacggcttt attgccctcg ttctggtagc
    10561 tgccgtcgtc ggatttgccg aagggatgga tgcacaggcc gtcctgcact ctatacaaaa
    10621 tggtatcggc agcacgctcg gtgggctggc aatgatcctc ggtttcgggg ccatgttagg
    10681 caagctgatt tctgatacgg gtgcggcaca acgtatcgcc actacgctga ttgctacttt
    10741 tggtaaaaaa cgcgtgcaat gggcgctagt gatcactggt ctggttgtgg gcctcgccat
    10801 gttttttgaa gtgggttttg tcctgctgtt gccgttggta tttaccatcg tagcatcatc
    10861 aggattaccc ctgttgtatg ttggcgtacc aatggtagca gcgctctctg taacccactg
    10921 ttttctgccg ccacatccag ggcctactgc catcgcgact atctttgagg ctaatctcgg
    10981 aacgacttta ctgtatggat ttatcattac cattccgaca gttattgtcg caggaccgct
    11041 gttttctaaa ctgctaactc gctttgagaa agcaccaccg gaaggcttat ttaatcctca
    11101 tctgtttagc gaagaggaga tgccctcctt ctggaacagt attttcgctg ccgtgatccc
    11161 ggtcatcctg atggctatcg ccgccgtttg tgaaattacg ttaccgaaaa ctaacaccgt
    11221 gcgcctcttc tttgaatttg tcggtaaccc tgccgttgcg ctgtttattg ccattgttat
    11281 tgcgattttc acactgggcc gacgtaatgg acgcaccatc gagcaaatca tggatatcat
    11341 tggggattct ataggcgcta tcgcgatgat tgtgtttatt atcgctggcg gcggcgcgtt
    11401 taagcaggta ttagtagata gcggtgtcgg gcactatatt tcacacttaa tgaccggaac
    11461 tacgctttcg ccgttattga tgtgctggac tgttgcggcg ctgttgcgta tcgctctggg
    11521 ctctgccacc gtcgcggcca ttaccaccgc gggtgtggtg ttgccgatta tcaacgttac
    11581 ccatgccgat cccgctttaa tggtactggc aaccggtgcg ggcagcgtga tcgcgtcaca
    11641 cgtaaacgac cctggcttct ggctatttaa agggtatttt aatctgacgg ttggtgaaac
    11701 gttgcgtacc tggacggtga tggaaaccct tatttctatt atgggtttgc tgggcgtgtt
    11761 agccattaac gccgtattgc actgaaagaa taaaatattg tgatgtagtt tcgggggaag
    11821 attgaagatt cccccgacgt tgggagaagt tatgcgcaat cacagaattt ctttacagga
    11881 tatcgctacg ctggctggcg taacaaaaat gaccgtgagt cgttatatcc gctcgccgaa
    11941 aaaggtggca aaggaaacag gcgagcgcat cgccaaaatt atggaagaga tcaattatat
    12001 ccctaatcgc gctcccggca tgttgttgaa tgcacaaagt tataccctcg gtatcttgat
    12061 tccgtcattt caaaatcaac tcttcgctga cattctcgcg ggaatagaat ccgtcacctc
    12121 tgagcataat tatcagaccc ttattgctaa ttacaattac gatcgcgatt ccgaagaaga
    12181 gtcggtgatt aacctgctct cttataacat tgacgggatt atactttcgg aaaaatatca
    12241 caccatcagg acggtgaaat ttctgcgttc agccaccatt ccggtcgtgg aattgatgga
    12301 tgtacaggga gaacggctgg atatggaggt cggttttgat aatcgtcagg ccgcttttga
    12361 catggtgtgt accatgctgg agaagcgagt caggcacaaa attctgtatc tcggttccaa
    12421 agatgacacc cgcgatgaac agcgttatca ggggtattgc gatgcgatga tgctgcataa
    12481 tctttcccca ttacgcatga atccacgcgc catctcatcc attcatttag gaatgcagtt
    12541 aatgcgtgat gcccttagcg caaacccgga tttagacggt gtattttgca ccaatgacga
    12601 tattgcgatg ggtgcgcttc tgctgtgccg cgagcgaaac ctcgccgtgc cagaacaaat
    12661 ttccattgcg ggttttcatg ggctggagat tggcaggcaa atgatcccaa gcctcgccag
    12721 cgtaattact ccccgttttg atattggtcg gatggccgcc caaatgttgc tgagcaaaat
    12781 taaaaataac gatcacaacc acaacactgt tgacctcggg tatcagattt atcacggcaa
    12841 cacgctttag gttattttct cttcattaca cgtattctct ttgccggacg ttgcctatac
    12901 tccaggcagg aaactggagg atatctcatg agcgaacccc tgttaattgc ccgcacgccg
    12961 gacacagaac tatttttact gccgggaatg gctaaccgtc acgggctgat tactggcgca
    13021 acggggacgg gtaaaactgt cacgctgcaa aaactggcgg agtctctatc ggaaatcggt
    13081 gttccggtgt ttatggccga tgtgaaaggc gatctgaccg gcatcgcgca ggcaggaacg
    13141 gcgtcggaaa aactgctcgc aaggcttaaa aatatcggcg taaatgactg acaaccgcat
    13201 gctaatccgg tggtggtgtg ggatatcttt ggcgagaagg gccatccggt gcgggcgacg
    13261 gtttcggatc tggggccgct gttgctggcg cggctgttga atctcaacga tgtgcaatct
    13321 ggcgtgctga atatcatctt ccgcattgct gatgatcagg ggctgttact gctcgacttt
    13381 aaagatttgc gggcgattac ccagtacatc ggcgataacg ccaaatcttt ccagaatcag
    13441 tacggaaata tcagtagcgc atcggttggt gccatccagc gcgggctgtt gtcgctggaa
    13501 cagcaaggcg cagcacactt ctttggtgag ccgatgctgg atatcaaaga ctggatgcgc
    13561 accgatacca acggtaaagg cgttatcaat atcctcagcg ccgagaagct ttatcagatg
    13621 ccgaaactgt acgccgccag cctgttgtgg atgctttcag agttgtatga acaattgccg
    13681 gaagcgggcg atctggagaa accaaaactg gtgtttttct tcgacgaagc gcatctgctg
    13741 tttaacgatg caccgcaggt actgctggat aagattgagc aggtgataag gcttattcgc
    13801 tcaaaaggcg tgggcgtctg gttcgtttcg caaaacccgt cagatatccc ggataacgtg
    13861 ctcgggcagc tcggtaatcg cgttcaacac gctttgcgtg cttttacgcc taaagatcag
    13921 aaagcagtaa aagctgcggc gcaaaccatg cgccccaacc ctgcatttga tacggaaaag
    13981 gcgattcagg aactaggaac cggcgaagcg ttaatctcgt ttctcgatgc gaaaggaagc
    14041 ccttcagtgg tggaacgggc gatggtgatt gccccctgtt cacgaatggg gccggtgacg
    14101 gaagatgagc gcaatggctt gattaaccac tccccggtgt atggcaagta cgaggatgac
    14161 gtggaccggg aatccgccta tgagatgttg caaaaaggct ttcaggccag tatcgagcag
    14221 caaaataatc ctcccgcgaa agggaaagag gtggcggtgg atgacggtat tctcggtgga
    14281 ttgaaggata ttttgtttgg cactactgga ccacgcggcg ggaagaaaga tggtgtggtg
    14341 caaacaatgg cgaaaagcgc cgctcgccag gtgacgaatc agattgtacg cgggatgttg
    14401 gggagtttgc tgggggggag aagaaggtaa gtcaggtgcg atttgtcttt gcctgatgcg
    14461 acgctggcgc gtcttatcat gcctacacca ctgcaatata ttggattttc ataatttttc
    14521 aggaatgaac gaagcacact ttgctaacaa taataaaggg agctttcgct ccctttattc
    14581 gttcattcgg ttacgatttt ctcattaaca gccacaggct gattaagaag aagctggcgc
    14641 ttggcaacag tgcgccgata atcggcggga tgccgtaaac caacgtcagc gggccgaaga
    14701 tctggtccag tacgtagaag acaaaaccga agctgatacc ggtgaccaca cgcacgccca
    14761 tcggtacgct acgcagtggg ccaaagatga acgacagcgc catcagcatc atcaccgcca
    14821 cggatagcgg ctggaagatt ttgctccaca tattgagctg ataacgtccg gcatcctgac
    14881 cgctcgactt cagatacttc acgtagttgt gcaaaccgct gattgagagt gcatccgggt
    14941 ccagcgccac cacgcccagt ttgtctggcg tgaggttggt tttccaggtg ccgctcaccg
    15001 tctgcgaacc ggtaatctgt ttcgaattgg tcagatcaga ttcatccacc tgcgacagac
    15061 gccagacttt atgttccggg tcaaacttcg cggtagcggc atagcgtacg gattgcagac
    15121 gacgattctc gttaaaggca taaatgctga tgccacctaa ctcttcgtca cctttaaccc
    15181 gctcaatgta gacgaagttg ttgccatctt tcgcccataa gccttgctgg gtagagagca
    15241 acgagccgcc gtacatcgcc tgcgcacggt agttacgcgc catctgctcg ccctgcggcg
    15301 cgacccattc accaatcgcc atcgtcagta agaccagcgg aatggcggtt ttcatcaccg
    15361 acagcgccac ctgcatacgg gtaaaaccag aagcctgcat caccaccagt tcgctgcgct
    15421 gcgccagcat cccaagacca agcaacgccc caagcagagc cgccatcggg aagaagattt
    15481 gcacatcttt cggcacgctc agcaaggtat acattcctgc gcctaacgcg tcgtaactcc
    15541 cctgcccggc ttttttcagc tgatcgacaa acttgataat gcccgacagc gacaccagca
    15601 tgaacagcgt catcatgatg gtggtgaaaa tagttttacc gatatagcgg tcaagtacgc
    15661 caaaaggttg catcacaccg ctcctttacg cgaaaaactg gcgcgcaggc ggcggaccgg
    15721 cacggtgtcc caaaggttga gaacaatcgc caaagccaga taaatcaggt taacggtcca
    15781 catccacagc gtcgggtcca gcttaccttt accgccgttc gatttcaggg aggtctggat
    15841 caggaagaaa agtagataca gcagcatggc tggcagcatc gacagtacgc gtccctgacg
    15901 cgggttaacc acgctcagcg gtacgaccat aagtgccatc ataaacacgg tgaataccaa
    15961 cgtgatacgc cagttcagtt ctgcgcgagc acgatcggta tcagtgttcc acaatgtacg
    16021 catgtccatc tggtcggtat cgttcgggtc gagcgccacc gcctggtgac caatgatcgc
    16081 ctgataatcc tggaagtccg taatgcggaa atcacgtaac agtgcagtgc cttcgaagcg
    16141 cgttccctgg ttgagagtga cgacctggga gccgtcgcgc agctgggtta aatgtccgga
    16201 atcggccacc accacagaag gacgagcatt accttttggt cgaatttgcg cgaggaacac
    16261 atctttgaaa tcgctgccgt caacgctttc gatgaacagc accgagctgc cattagtcgc
    16321 ttgctggaat tgcccttgcg ccagcgccgc catgccaggg ttcgctttcg cttctgctaa
    16381 cacttcatcc tgatgacgcg atgaccacgg tcccgcccac atcacgttaa ccgccgcgac
    16441 gattgccgtg aataccgcaa ggatcattgc cgctttcacc agaaccgctt tgctcaggcc
    16501 gcaggcatgc attaccgtaa tttcactttc ggtatacagt ttgcccagcg tcatcagcag
    16561 cccgaggaac aggcttaatg gcaggataag ctgcgccatt tccggcacgc ccaacccgag
    16621 aagggagagc accagattcg ccggaatatc gccgtcaacc gctgcgccga ggatcctcac
    16681 taacttttga cagaagaaga tcaaaagcaa gatgaagagt atcgccagct ggcttttgag
    16741 cgcctcccgc accagatatc ttatgattat cactttaaat acgcccgtaa aaactcgtct
    16801 tttgcaggat tttagcttgt ttcatggctt aaacgtcatt tattctcttg agtcgtcgaa
    16861 atcgtcgcta agataattat actcaacgga ttcacctctc agattttgtt ctgacgtgcc
    16921 aatgccgtaa taacgttaag attaacacga agtcatcgca acagcggaca tgagttacga
    16981 aagcttgcaa ttctatctgt agccaccgcc gttgtcttta agattcagga gcgtagtgca
    17041 tggagtttag tgtaaaaagc ggtagcccgg agaaacagcg gagtgcctgc atcgtcgtgg
    17101 gcgtcttcga accacgtcgc ctttctccga ttgcagaaca gctcgataaa atcagcgatg
    17161 ggtacatcag cgccctgcta cgtcggggcg aactggaagg aaaaccgggg cagacattgt
    17221 tgctgcacca tgttccgaat gtactttccg agcgaattct ccttattggt tgcggcaaag
    17281 aacgtgagct ggatgagcgt cagtacaaac aagtcattca gaaaaccatt aatacgctga
    17341 atgatactgg ctcaatggaa gcggtctgct ttctgactga actgcacgtt aaaggccgta
    17401 acaactactg gaaagtgcgt caggctgtcg agacggcaaa agagacgctc tacagtttcg
    17461 atcagctgaa aacgaacaag agcgaaccgc gtcgtccgct gcgtaagatg gtgttcaacg
    17521 tgccgacccg ccgtgaactg accagcggtg agcgcgcgat ccagcacggt ctggcgattg
    17581 ccgccgggat taaagcagca aaagatctcg gcaatatgcc gccgaatatc tgtaacgccg
    17641 cttacctcgc ttcacaagcg cgccagctgg ctgacagcta cagcaagaat gtcatcaccc
    17701 gcgttatcgg cgaacagcag atgaaagagc tggggatgca ttcctatctg gcggtcggtc
    17761 agggttcgca aaacgaatcg ctgatgtcgg tgattgagta caaaggcaac gcgtcggaag
    17821 atgcacgccc aatcgtgctg gtgggtaaag gtttaacctt cgactccggc ggtatctcga
    17881 tcaagccttc agaaggcatg gatgagatga agtacgatat gtgcggtgcg gcagcggttt
    17941 acggcgtgat gcggatggtc gcggagctac aactgccgat taacgttatc ggcgtgttgg
    18001 caggctgcga aaacatgcct ggcggacgag cctatcgtcc gggcgatgtg ttaaccacca
    18061 tgtccggtca aaccgttgaa gtgctgaaca ccgacgctga aggccgcctg gtactgtgcg
    18121 acgtgttaac ttacgttgag cgttttgagc cggaagcggt gattgacgtg gcgacgctga
    18181 ccggtgcctg cgtgatcgcg ctgggtcatc atattactgg tctgatggcg aaccataatc
    18241 cgctggccca tgaactgatt gccgcgtctg aacaatccgg tgaccgcgca tggcgcttac
    18301 cgctgggtga cgagtatcag gaacaactgg agtccaattt tgccgatatg gcgaacattg
    18361 gcggtcgtcc tggtggggcg attaccgcag gttgcttcct gtcacgcttt acccgtaagt
    18421 acaactgggc gcacctggat atcgccggta ccgcctggcg ttctggtaaa gcaaaaggcg
    18481 ccaccggtcg tccggtagcg ttgctggcac agttcctgtt aaaccgcgct gggtttaacg
    18541 gcgaagagta attgcgtcag gcaaggctgt tattgccgga tgcggcgtga acgccttatc
    18601 cgacctacac agcactgaac tcgtaggcct gataagacac aacagcgtcg catcaggcaa
    18661 tgctgttatt gtcggatgcg gcatgaacgc cttatccgac ctacacagca ccgaactcgt
    18721 aagcctgata agacgcggca gcgtcgcatc aggcaatgct gttattgtcg gatgcggcgt
    18781 gagcgcctta tccgacctac acacagcact gaactcgtag gcctgataag acacaacagc
    18841 gtcgcatcag gcgctgcggt gtatacctga tgcgtattta aatccaccac aagaagcccc
    18901 atttatgaaa aacgcgacgt tctaccttct ggacaatgac accaccgtcg atggcttaag
    18961 cgccgttgag caactggtgt gtgaaattgc cgcagaacgt tggcgcagcg gtaagcgcgt
    19021 gctcatcgcc tgtgaagatg aaaagcaggc ttaccggctg gatgaagccc tgtgggcgcg
    19081 tccggcagaa agctttgttc cgcataattt agcgggagaa ggaccgcgcg gcggtgcgcc
    19141 ggtggagatc gcctggccgc aaaaacgcag cagcagcccg cgcgatatat tgattagtct
    19201 gcgaacaagc tttgcagatt ttgccaccgc tttcacagaa gtggtagact tcgttcctta
    19261 tgaagattct ctgaaacaac tggcgcgcga acgctataaa gcctaccgcg tggctggttt
    19321 caacctgaat acggcaacct ggaaataatg gaaaagacat ataacccaca agatatcgaa
    19381 cagccgcttt acgagcactg ggaaaagcag ggctacttta agcctaatgg cgatgaaagc
    19441 caggaaagtt tctgcatcat gatcccgccg ccgaacgtca ccggcagttt gcatatgggt
    19501 cacgccttcc agcaaaccat catggatacc atgatccgct atcagcgcat gcagggcaaa
    19561 aacaccctgt ggcaggtcgg tactgaccac gccgggatcg ctacccagat ggtcgttgag
    19621 cgcaagattg ccgcagaaga aggtaaaacc cgtcacgact acggccgcga agctttcatc
    19681 gacaaaattt gggaatggaa agcggaatcc ggcggcacca ttacccgtca gatgcgccgt
    19741 ctcggcaact ccgtggactg ggagcgtgaa cgcttcacga tggacgaagg cctgtccaat
    19801 gcggtgaaag aagttttcgt tcgtctgtat aaagaagacc tgatttaccg tggcaaacgc
    19861 ctggtaaact gggacccgaa actgcgcacc gctatctctg acctggaagt ggaaaaccgc
    19921 gaatcgaaag gttcgatgtg gcacatccgc tatccgctgg ctgacggtgc gaaaaccgca
    19981 gacggtaaag attacctggt ggttgcgact acccgtccgg aaaccctgct gggcgatacc
    20041 ggtgtagccg ttaacccgga agatccgcgt tacaaagacc tgattggcaa atatgtcatt
    20101 ctaccgctgg ttaaccgtcg tattccgatc gttggcgacg aacacgccga catggaaaaa
    20161 ggcaccggct gcgtgaagat caccccggcg cacgacttta acgactatga agtcggtaaa
    20221 cgtcacgccc tgccgatgat caacatcctg acctttgacg gcgatatccg tgaaagcgcc
    20281 caggtgttcg ataccaaagg taacgaatct gacgtttatt ccagcgaaat cccggcagag
    20341 ttccagaaac tggagcgttt tgctgcacgt aaagcagtcg ttgctgcggt tgacgcgctc
    20401 ggcctgctgg aagaaattaa accgcacgac ctgaccgttc cttatggcga ccgtggtggc
    20461 gtggttatcg aaccgatgct gaccgaccag tggtacgtac gtgccgacgt gctggcgaaa
    20521 ccggcagttg aagcggttga gaatggcgac attcagttcg taccgaagca gtacgaaaac
    20581 atgtatttct cctggatgcg cgatattcag gactggtgta tctctcgtca gctatggtgg
    20641 ggtcaccgta tcccggcatg gtatgacgaa gcgggtaatg tttatgttgg tcgcaacgaa
    20701 gacgaagtgc gtaaagaaaa taacctcggt gctgatgttg cgctgcgtca ggatgaagac
    20761 gttctcgaca cctggttctc ctccgcgctg tggaccttct ccacgctcgg ctggccggaa
    20821 aacaccgacg cactgcgcca gttccaccca accagcgtga tggtatctgg ttttgacatc
    20881 atcttcttct ggattgcccg catgatcatg atgaccatgc acttcatcaa agatgaaaat
    20941 ggcaaaccgc aggtgccgtt ccacaccgtt tacatgaccg gcctgattcg tgatgacgaa
    21001 ggccagaaga tgtccaaatc caaaggtaac gtgattgacc cactggatat ggttgacggt
    21061 atttcgctgc cggaactgct ggaaaaacgt accggcaata tgatgcagcc gcagctggcg
    21121 gacaaaatcc gtaagcgcac cgagaagcag ttcccgaacg gcattgagcc gcacggtact
    21181 gacgcgctgc gcttcaccct ggcggcgctg gcttctaccg gtcgtgacat caactgggat
    21241 atgaagcgtc tggaaggcta ccgtaacttc tgtaacaagc tgtggaacgc cagccgtttt
    21301 gtgctgatga acacggaagg tcaggattgc ggcttcaacg gcggtgaaat gacgctgtcg
    21361 ctggcggatc gctggattct ggcggagttc aaccagacca tcaaagcgta ccgcgaagcg
    21421 ctggacagct tccgcttcga tatcgccgca ggcattctgt atgagttcac ctggaaccag
    21481 ttctgcgact ggtatctgga gctgaccaag ccggtaatga acggtggaac agaagccgaa
    21541 ctgcgcggta ctcgccatac gctggtgact gtactggaag gtctgctgcg cctcgcgcat
    21601 ccgatcattc cgttcatcac cgaaaccatc tggcagcgtg tgaaagtact ttgcggtatc
    21661 actgccgaca ccatcatgct gcagccgttc ccgcagtacg atgcgtctca ggttgatgaa
    21721 gccgcactgg ccgacaccga gtggctgaaa caggcgatcg ttgctgtacg taacatccgt
    21781 gcagaaatga acatcgcacc gggtaaaccg ctggagctgc tgctgcgtgg ttgcagcgcg
    21841 gatgcagaac gtcgcgtaaa tgaaaaccgt ggcttcctgc aaacgctggc gcgtctggaa
    21901 agcatcaccg tgctgcctgc tgatgataaa ggtccggttt ccgttacaaa gatcgtcgac
    21961 ggcgcagaac tgctgatccc gatggcaggc ctcatcaaca aagaagatga gctggcacgt
    22021 ctggcgaaag aagtggcgaa gatcgaaggc gaaatcagcc gtattgagaa caagctggcg
    22081 aacgaaggct ttgtcgcccg cgcaccggaa gcggtcatcg cgaaagagcg tgagaagctg
    22141 gaaggctacg cagaagcgaa agcgaagctg attgaacagc aggctgttat cgccgcgctg
    22201 taatactcat agtgaaaacg aaggccggag catgctccgg cctttttatt tagataaacg
    22261 gaacatacgg cataataccg cgagatatcc acattaacga accgctatcc tgcggtttgt
    22321 catcattggt taactccagc gaatcaaggt cgccctggac atgggtattg ttagcaatcc
    22381 agctaaccat ccacattttc agccagggat aagccagacc acaggtaatt gaggccccca
    22441 ttaataacgc caacatgcgc agaagcatac cgatcgcggt aacagatgaa tgaaaacgaa
    22501 tagcgccatt tgccagtgtc agattattca ggaacaaact gcgtaacgtg acatagagat
    22561 aactggcaaa cacaaggatc gcgacaaaat acaggaaata agaagccata atctgcgggt
    22621 aatattgtaa tacaaactca ctgccagcat cagacctacc caacatggtc atcatcatta
    22681 actgctgaaa tagtggtgcg atcataatac caatcacaat gatgaaagga accatggtta
    22741 atattgcgag catgcaacct ttaatgcatt cttttacgtt tacctgaata gaaaacttat
    22801 gaatgccaaa agttgcatta ttacccagca atggcatcca tttactatag gttataccat
    22861 ggacaacacc tagcccgatg gcagaaagca aacttaatgc gactaagtta aagaataacc
    22921 ctcctatcga tgtcgtcact tgtgcaataa gatacagtgc gaaccaaaaa actaacgcca
    22981 acagaacagg cagtcctagc ataacccacc atgcacgcat cattgagcag ttaaagccaa
    23041 agcgaatgtc attcagtgag gtcatgttcg cctgatactg caggctcttc actgccatac
    23101 aaggaatacc gcagatcaag agagcaaaca ggaataaacc gagcgcaaag tgttgacctg
    23161 caaaacaaga aatactgaga agataaacca ccacaataag taacatgcta acaaaaatgg
    23221 caccacctgt tcccttataa ctaaatggtt gccccttgag tgtcacatgt tgataaatat
    23281 aacgacggca ttttatcaat gcccacggtc catatattcc cagagtaata attgtcagta
    23341 aaaaattcac aagacaaata agaaaatact caccaccttt gcctgtgaaa acaaacgagt
    23401 gccgagaatt atctttcccg atgtttacat tattcatgaa ctatatccct aaaataaatt
    23461 ccattcatac cgataataat tccagttaat tgatactggt tcgcctagaa tacgctttat
    23521 ctcagagaga tatgcttaat attttctgat ttatcttctt cttgcaccac ctaagcgagg
    23581 gtggtattaa agtgagctat aaatcataca tttgaaattg agctatgcta tgaatgttgt
    23641 cgcttctccc gcactccgtt tgcgcaaact taccgtcgcc gataatcctg ctatcgccca
    23701 cgtcattcgt caggtatccg ccgaatacgg tcttaccgct gataaaggct acaccgtcgc
    23761 cgatccgaac cttgacgagc tgtatcaagt gtatagtcag cctggccacg catattgggt
    23821 ggttgagtac gagggtgaag tggtcggcgg cggtgggatt gcgccattaa ccgggagtga
    23881 gtcagacatt tgcgaactgc aaaagatgta ttttcttccg gctatccgcg gcaaagggct
    23941 ggcaaaaaaa ctggccttaa tggcaatgga gcaggcgcga gaaatgggtt tcaaacgctg
    24001 ctatctggaa acgaccgctt ttttaaagga agccattgcg ctttatgagc atttgggctt
    24061 tgagcatatc gactatgcgc ttggctgcac gggtcatgtc gattgtgaag tacggatgct
    24121 acgcaaactt tgaaaaatgc ccgcgagctt agactcgcgg gcattcgaac attaaccacg
    24181 tgctttttga gtatgctgga tgtgacgtaa atgccttatc cgggctacaa attcaccagg
    24241 ttgcaggaac tacctgggcc tgatatgcgt agcgcatcag gtaacgcagc ctttgtcgtc
    24301 aacgcttaat tagtgacgaa taccgtcatc gtcttcatcg acaaaatctt catcgtcacc
    24361 gtcttcgccg ttaggatctt caaagtaagt gccccaaccg tcgtactcaa cgtcaaattt
    24421 ctccgccagc gtcatcagtt gttcaacctg ggcatcgatc agatcggcat tcaacgcaca
    24481 ctcgctgagg atgtcgcagc aaatcacgat gtcaccatct tcaacttcca gctcttctgg
    24541 atcggtcact tcgtaaccga gtttaaacgc ttcaactgct gctttttcca gggtttccag
    24601 atcgtctgcg gaaagatggt gttcgatggt gtacagcgcg tccggatcgc tgccatcttc
    24661 cagtaattct tcaataatca aacgtgtttc ttcacgctgt tcttccagtt gttccgggtt
    24721 tgccatggct cattcctctt aatgtcctgc cgatactctt attgtcacac accactgaca
    24781 ttgcctccac ctttgtgaca aagatttatg ctttagactt gcaaatgaat aatcatccat
    24841 ataaattgaa ttttaattca ttgaggcatt agccacagga gggatctatg tccgggtttt
    24901 atcataagca tttcctgaaa ttactcgatt tcacgccagc tgaactcaac agcttgttgc
    24961 agttagccgc gaagctgaaa gccgataaga aaagcggtaa agaagaagcc agactcactg
    25021 gcaaaaacat cgcgctcatc ttcgaaaaag actcgacccg tacccgatgc tctttcgaag
    25081 ttgccgcata tgaccagggt gctcgcgtta cttatctcgg cccaagcggc agccagattg
    25141 gtcacaaaga gtcgattaaa gacactgccc gcgtgcttgg tcgcatgtat gacggtattc
    25201 agtatcgcgg ctatggtcag gagattgtcg aaacactggc ggaatacgct ggcgtgccag
    25261 tatggaacgg cctgaccaat gagttccacc ccacgcagct gctggcggat ctcctcacca
    25321 tgcaggagca tttgcccggc aaagcattca acgaaatgac gctggtctat gcaggtgacg
    25381 cgcgtaacaa catgggcaat tcgatgctcg aagctgcgtc gcttaccggt ctggatttgc
    25441 gtctggtcgc gccacaggca tgctggccgg aagctgcgct ggtcgccgaa tgcagtgctc
    25501 tggcacaaaa acacgggggt aaaatcacct taacggaaga tatcgcctct ggggttaaag
    25561 gcgcagactt tatctatacc gatgtctggg tatcgatggg tgagcccaaa gagaaatggg
    25621 cggagcgtat tgccctgctg cgtgactatc aggtgaatag caaaatgatg gccctgaccg
    25681 gtaattcgca ggttaagttc ctccactgtc tgcctgcatt tcatgatgag caaaccacgc
    25741 tgggtaaaaa aatggccgca gaatttggtc tgtatggtgg aatggaagtc accgatgaag
    25801 tctttgaatc tccggcaagt attgtctttg accaggcaga aaaccggatg cacaccataa
    25861 aagctgttat ggtcgctaca ttagcaaaat aacgttttgc cgggcggcgg caccggtcgc
    25921 ccccggcagt gatcacttac gcttttaata gcttaactac tgctttccgt acctgagcgg
    25981 gggctcccac cgcacaaagc ggtttgtgaa cttcgccagg atagaaaacg acaaaatccc
    26041 cttcattaag aatcactgtt ttttcatcga tcccctgccc gataaaagca atatctttat
    26101 ctgccagcca gtcagtctct ggtacgcctg ctggttgcgt gctaaatgtc atcccttcct
    26161 gcccttttaa tacaatctgg atatcgagat aacgagcatg atactcagca cgacgcagtt
    26221 cacctggttc agtcgtatcc tcagaaatca gataaaacag acggtcgccg tcaatagcgt
    26281 gcttaccttt tgctgtctca tcgctgacat gtgatttgat gtactcaatt gcttcgcgaa
    26341 gttcttccgg cagccaggat tgtaagtgat ggatgttgcc aacaatcata atatcttcct
    26401 ttattaaaac aatgtttcac ttctttcctt atacgaaaaa aagccacctc tcaccataga
    26461 aaaatcatca ggttttgccc ttgcgcatgt ttattgctgg agatgttgta ctgatgttaa
    26521 gcaggagtaa ttttgtatgc agttttgctg cataaatctt ataacttagc tatgtatttt
    26581 ggtcctaata tcttgttatt gcatatgtta aaagaaaact ctcagtcaga tcacagttca
    26641 ttttcttagc ttttaatgcc cgtgctgtca tagttatccc gtgaaaatag tgctgtgaaa
    26701 aataatcacc ttatc
//
LOCUS       DAHHTG010000062        26671 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.062, whole genome shotgun sequence.
ACCESSION   DAHHTG010000062 DAHHTG010000000
VERSION     DAHHTG010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26671)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26671)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26671
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.062"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            36..800
                     /gene="ycaL"
                     /locus_tag="NBI74_002514"
     CDS             36..800
                     /gene="ycaL"
                     /locus_tag="NBI74_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase YcaL"
                     /protein_id="HCD1932833.1"
                     /translation="MKNTKLLLAIATSAALLTGCQNTHGINTDLAISSGLNAYKAATL
                     SDADAKAIANQGCVEMDSGNKVASKSSKYGKRLAKIAKALGNNINGTPVNYKVYMTSD
                     VNAWAMANGCVRVYSGLMDMMNDNEIEGVLGHELGHVALGHSLAEMKASYAIVAARDA
                     ISATSGVASQLSRSQLGDIAEGAINAKYSRDKESEADDFSFDLLKKRGISTQGLVGSF
                     EKLASLDGGHTQSMFDSHPPSTERAQHIRDRIASGK"
     gene            973..1656
                     /gene="cmk"
                     /locus_tag="NBI74_002515"
     CDS             973..1656
                     /gene="cmk"
                     /locus_tag="NBI74_002515"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HCD1932834.1"
                     /translation="MTAIAPVITIDGPSGAGKGTLCKAMAEALQWHLLDSGAIYRVLA
                     LAALHHHVDVASEDALVPLASHLDVRFVSTNGNLEVILEGEDVSGEIRTQEVANAASQ
                     VAAFPRVREALLRRQRAFRELPGLIADGRDMGTVVFPDAPVKIFLDASSEERAHRRML
                     QLQEKGFSVNFERLLAEIKERDDRDRNRAVAPLVPAADALVLDSTTLSIEQVIEKALQ
                     YARQKLALA"
     gene            1767..3440
                     /gene="rpsA"
                     /locus_tag="NBI74_002516"
     CDS             1767..3440
                     /gene="rpsA"
                     /locus_tag="NBI74_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HCD1932835.1"
                     /translation="MTESFAQLFEESLKEIETRPGSIVRGVVVAIDKDVVLVDAGLKS
                     ESAIPAEQFKNAQGELEIQVGDEVDVALDAVEDGFGETLLSREKAKRHEAWITLEKAY
                     EDAETVTGVINGKVKGGFTVELNGIRAFLPGSLVDVRPVRDTLHLEGKELEFKVIKLD
                     QKRNNVVVSRRAVIESENSAERDQLLENLQEGMEVKGIVKNLTDYGAFVDLGGVDGLL
                     HITDMAWKRVKHPSEIVNVGDEITVKVLKFDRERTRVSLGLKQLGEDPWVAIAKRYPE
                     GTKLTGRVTNLTDYGCFVEIEEGVEGLVHVSEMDWTNKNIHPSKVVNVGDVVEVMVLD
                     IDEERRRISLGLKQCKANPWQQFAETHNKGDRVEGKIKSITDFGIFIGLDGGIDGLVH
                     LSDISWNVAGEEAVREYKKGDEIAAVVLQVDAERERISLGVKQLAEDPFNNWVALNKK
                     GAIVTGKVTAVDAKGATVELADGVEGYLRASEASRDRVEDATLVLSVGDEVEAKFTGV
                     DRKNRAISLSVRAKDEADEKDAIATVNKQEDANFSNNAMAEAFKAAKGE"
     gene            3600..3884
                     /gene="ihfB"
                     /locus_tag="NBI74_002517"
     CDS             3600..3884
                     /gene="ihfB"
                     /locus_tag="NBI74_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HCD1932836.1"
                     /translation="MTKSELIERLATQQSHIPAKTVEDAVKEMLEHMASTLAQGERIE
                     IRGFGSFSLHYRAPRTGRNPKTGDKVELEGKYVPHFKPGKELRDRANIYG"
     gene            4090..6354
                     /locus_tag="NBI74_002518"
     CDS             4090..6354
                     /locus_tag="NBI74_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309023.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC family protein"
                     /protein_id="HCD1932837.1"
                     /translation="MKITTVGVCIICGIFPLLILPQLPGTVTLAFLTLFACVLAFIPV
                     KTVRYIALTLLFFVWGILAAKQILWAGETLTGATQDAIVEIIATDGMTTHYGQITHLQ
                     GRRIFPAPGLVLYGEYLPQAVCAGQVWSMKLKVRAVHGQLNDGGFDSQRYAIAQHQPL
                     TGRFLQASVIEPNCSLRAQYLASLQTTLQPYMWNAVILGLGMGERLSVPKEIKNIMRD
                     TGTAHLMAISGLHIAFAALLAAGLIRGGQVFLPGCWLHWQMPLIGGICCAAFYAWLTG
                     MQPPALRTVVALAIWGMLKLSGRQWSGWDVWICCLAAILLMDPVAILSQSLWLSAAAV
                     AALIFWYQWFPCPEWQLPPVLRAVVSLIHLQLGITLLLMPVQIVIFHGISLTSFIANL
                     FAIPLVTFITVPLILAAMVVHLSGPFILEQGLWFLADRSLALLFWGLKSLPEGWINIA
                     ERWQWLTFSPWFLLVVWRLNAWRTLPAMCVAVGLLMCLPLWQKPRPDEWQVYMLDVGQ
                     GLAMVIARNGKAILYDTGLAWPEGDSGQQLIIPWLHWHNLEPEGVILSHEHLDHRGGL
                     DSILHTWPMLWIRSPLNWEHHQPCVRGEAWQWQGLRFSAHWPLQGSNDKGNNHSCVVK
                     IDDGTNSILLTGDIEAPAEQKMLSRYWQQMQATLLQVPHHGSNTSSSLPLIQRVNGKV
                     ALASASRYNAWRLPSSKVKYRYHQQGYKWLDTPHQGQITVNFSAQGWWISSLREQILP
                     RWYHQWFGVPVDNG"
     gene            6391..8139
                     /gene="msbA"
                     /locus_tag="NBI74_002519"
     CDS             6391..8139
                     /gene="msbA"
                     /locus_tag="NBI74_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A ABC transporter ATP-binding
                     protein/permease MsbA"
                     /protein_id="HCD1932838.1"
                     /translation="MHNDKDLSTWQTFRRLWPTIAPFKAGLIVAGVALILNAASDTFM
                     LSLLKPLLDDGFGKTDRSVLMWMPLVVIGLMILRGITSYISSYCISWVSGKVVMTMRR
                     RLFGHMMGMPVSFFDKQSTGTLLSRITYDSEQVASSSSGALITVVREGASIIGLFIMM
                     FYYSWQLSIILIVLAPIVSIAIRVVSKRFRNISKNMQNTMGQVTTSAEQMLKGHKEVL
                     IFGGQEVETKRFDKVSNRMRLQGMKMVSASSISDPIIQLIASLALAFVLYAASFPSVM
                     DSLTAGTITVVFSSMIALMRPLKSLTNVNAQFQRGMAACQTLFTILDSEQEKDEGKRV
                     IERATGDVEFRNVTFTYPGRDVPALRNINLKIPAGKTVALVGRSGSGKSTIASLITRF
                     YDIDEGEILMDGHDLREYTLASLRNQVALVSQNVHLFNDTVANNIAYARTEQYSREQI
                     EEAARMAYAMDFINKMDNGLDTVIGENGVLLSGGQRQRIAIARALLRDSPILILDEAT
                     SALDTESERAIQAALDELQKNRTSLVIAHRLSTIEKADEIVVVEDGVIVERGTHNDLL
                     EHRGVYAQLHKMQFGQ"
     gene            8136..9122
                     /gene="lpxK"
                     /locus_tag="NBI74_002520"
     CDS             8136..9122
                     /gene="lpxK"
                     /locus_tag="NBI74_002520"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HCD1932839.1"
                     /translation="MIEKIWSGESPLWRLLLPLSWLYGLVSGAIRLCYKLKLKRAWRA
                     PVPVVVVGNLTAGGNGKTPVVVWLVEQLQQRGIRVGVVSRGYGGKAESYPLLLSADTT
                     TAQAGDEPVLIYQRTGAPVAVSPVRSDAVKAILAQHPDVQIIVTDDGLQHYRLARDVE
                     IVVIDGVRRFGNGWWLPAGPMRERAGRLKLVDAVIVNGGVPRSGEIPMHLLPGQAVNL
                     RTGTRCDVAQLEHVVAIAGIGHPPRFFATLKMCGVQPEKCVPLADHQFLNHADVSALV
                     SAGQTLVMTEKDAVKCRAFAEENWWYLPVDAQLSGDEPAKLLAQLTSLASGN"
     gene            9159..10391
                     /locus_tag="NBI74_002521"
     CDS             9159..10391
                     /locus_tag="NBI74_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcaQ family DNA glycosylase"
                     /protein_id="HCD1932840.1"
                     /translation="MSLPHLSLADARNLHLAAQGLLNKPHRRASLEDIPATISRMSLL
                     QIDTINIVARSPYLVLFSRLGNYPAQWLDESLARGELMEYWAHEACFMPRSDFRLIRH
                     RMLAPEKMGWKYKDAWMQEHAAEIALLIQHIHDRGPVRSADFEHPRKGASGWWEWKPH
                     KRHLEGLFTAGKVMVIERRNFQRVYDLTHRVMPDWDDERDLVSQAEAEIIMLDNSARS
                     LGIFREQWLADYYRLKRPALAAWREARAEQQQIIAVHVEKLGNLWLHADLLPLLERAL
                     AGKLTATHSAVLSPFDPVVWDRKRAEQLFDFSYRLECYTPAPKRQYGYFVLPLLHRGQ
                     LVGRMDSKMHRQKGILEVISLWLQEGIKPTTTLQKGLRQAITDFANWQQATRVTLGRC
                     SQGLFTDCRAGWEIDPVA"
     gene            10443..10625
                     /gene="ycaR"
                     /locus_tag="NBI74_002522"
     CDS             10443..10625
                     /gene="ycaR"
                     /locus_tag="NBI74_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YcaR"
                     /protein_id="HCD1932841.1"
                     /translation="MDHRLLEIIACPVCNGKLWYNQEKQELICKLDNLAFPLRDGIPV
                     LLETEARVLTADEGKS"
     gene            10622..11368
                     /gene="kdsB"
                     /locus_tag="NBI74_002523"
     CDS             10622..11368
                     /gene="kdsB"
                     /locus_tag="NBI74_002523"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HCD1932842.1"
                     /translation="MSFVVIIPARYASTRLPGKPLVDINGKPMIVHVLERARESGAER
                     IIVATDHEDVARAVEAAGGEVCMTRADHQSGTERLAEVVEKCAFSDDTVIVNVQGDEP
                     MIPATIIRQVADNLAQRQVGMATLAVPIHNAEEAFNPNAVKVVLDAEGYALYFSRATI
                     PWDRDRFAKDLETVGDNFLRHLGIYGYRAGFIRRYVTWQPSPLEHIEMLEQLRVLWYG
                     EKIHVAVAQEVPGTGVDTPEDLERVRAEMR"
     gene            11522..12415
                     /locus_tag="NBI74_002524"
     CDS             11522..12415
                     /locus_tag="NBI74_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbJ family phosphotransferase"
                     /protein_id="HCD1932843.1"
                     /translation="MEQLRAELSHLLGEKLSRIECVNEKADTALWALYDSQGNPMPLM
                     ARSFSTPGKARQLAWKTTMLARSGTVRMPTIYGVMTHEEHPGPDVLLLERMRGVSVEA
                     PARTPERWEQLKDQIVEALLAWHRQDSRGCVGAVDNTQENFWPSWYRQHVEVLWTTLN
                     QFNNTGLSMQDKRILFRTRECLPALFEGFNDNCVLIHGNFCLRSMLKDSRSDQLLAMV
                     GPGLMLWAPREYELFRLMDNSLAEDLLWSYLQRAPVAESFIWRRWLYVLWDEVAQLVN
                     TGRFNRRNFDLASKSLLPWLA"
     gene            complement(12392..13171)
                     /gene="elyC"
                     /locus_tag="NBI74_002525"
     CDS             complement(12392..13171)
                     /gene="elyC"
                     /locus_tag="NBI74_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope biogenesis factor ElyC"
                     /protein_id="HCD1932844.1"
                     /translation="MLFTLKKVIGNMLLPLPLMLLIIGAGLALLWFSRFQKTGKIFIS
                     IGWLALLLLSLQPVADRLLRPIESTYPTWNNSQKVDYIVVLGGGYTWNPQWAPSSNLI
                     NNSLPRLNEGIRLWRENPGSKLIFTGGVAKTNTVSTAEVGARVAQSLGVPREQIITLD
                     LPKDTEEEAAAVKQAIGDAPFLLVTSASHLPRAMIFFQQEGLNPLPAPANQLAIDSPL
                     NPWERAIPSPVWLMHSDRVGYETLGRIWQWLKGSSGEPRQE"
     gene            13307..14092
                     /gene="cmoM"
                     /locus_tag="NBI74_002526"
     CDS             13307..14092
                     /gene="cmoM"
                     /locus_tag="NBI74_002526"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine 5-oxyacetic acid(34)
                     methyltransferase CmoM"
                     /protein_id="HCD1932845.1"
                     /translation="MQDRNFDDIAEKFSRNIYGTTKGQLRQAILWQDLDRVLAEIGPQ
                     KLRVLDAGGGEGQTAIKMAERGHQVILCDLSAQMIDRAKQAAEAKGVSDNMQFIHCAA
                     QDVASHLETPVDLILFHAVLEWVADPRSVLQTLWSVLRPGGVLSLMFYNAHGLLMHNM
                     VAGNFDYVQAGMPKKKKRTLSPDYPRDPAQVYLWLEEAGWQILGKTGVRVFHDYLREK
                     HQQRDCYEALLELETRYCRQEPYITLGRYIHVTARKPQSKDKV"
     gene            14089..15411
                     /gene="mukF"
                     /locus_tag="NBI74_002527"
     CDS             14089..15411
                     /gene="mukF"
                     /locus_tag="NBI74_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukF"
                     /protein_id="HCD1932846.1"
                     /translation="MSEFSQTVPELVAWARKNDFSISLPVDRLSFLLAVATLNGERLD
                     GEMSEGELVDAFRHVSDAFEQTSETIGVRANNAINDMVRQRLLNRFTSEQAEGNAIYR
                     LTPLGIGITDYYIRQREFSTLRLSMQLSIVAGELKRAADAAEEGGDEFHWHRNVYAPL
                     KYSVAEIFDSIDLTQRLMDEQQQQVKDDIAQLLNKDWRAAISSCELLLSETSGTLREL
                     QDTLEAAGDKLQANLLRIQDATMTHDDLHFVDRLVFDLQSKLDRIISWGQQSIDLWIG
                     YDRHVHKFIRTAIDMDKNRVFAQRLRQSVQTYFDEPWALTYANADRLLDMRDEEMVLR
                     DEEVTGELPEDLEYEEFNEIREQLAAIIEEQLAVYKTRQVPLDLGLVVREYLSQYPRA
                     RHFDVARIVIDQAVRLGVAQADFTGLPAKWQPINDYGAKVQAHVIDKY"
     gene            15392..16096
                     /gene="mukE"
                     /locus_tag="NBI74_002528"
     CDS             15392..16096
                     /gene="mukE"
                     /locus_tag="NBI74_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukE"
                     /protein_id="HCD1932847.1"
                     /translation="MSSTNIEQVMPVKLAQALANPLFPALDSALRSGRHIGLDELDNH
                     AFLMDFQEYLEEFYARYNVELIRAPEGFFYLRPRSTTLIPRSVLSELDMMVGKILCYL
                     YLSPERLANEGIFTQQELYDELLTLADEAKLLKLVNNRSTGSDVDRQKLQEKVRSSLN
                     RLRRLGMVWFMGHDSSKFRITESVFRFGADVRAGDDPREAQRRLIRDGEAMPIENHLQ
                     LNDETEESQPDSGEEE"
     gene            16096..20556
                     /gene="mukB"
                     /locus_tag="NBI74_002529"
     CDS             16096..20556
                     /gene="mukB"
                     /locus_tag="NBI74_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukB"
                     /protein_id="HCD1932848.1"
                     /translation="MIERGKFRSLTLINWNGFFARTFDLDELVTTLSGGNGAGKSTTM
                     AAFVTALIPDLTLLHFRNTTEAGATSGSRDKGLHGKLKAGVCYSMLDTINSRHQRVVV
                     GVRLQQVAGRDRKVDIKPFAIQGLPMSVQPTQLVTETLNERQARVLPLNELKDKLEAM
                     EGVQFKQFNSITDYHSLMFDLGIIARRLRSASDRSKFYRLIEASLYGGISSAITRSLR
                     DYLLPENSGVRKAFQDMEAALRENRMTLEAIRVTQSDRDLFKHLISEATNYVAADYMR
                     HANERRVHLDKALEFRRELHTSRQQLAAEQYKHVDMARELAEHNGAEGDLEADYQAAS
                     DHLNLVQTALRQQEKIERYEADLDELQIRLEEQNEVVAEAIERQEENEARAEAAELEV
                     DELKSQLADYQQALDVQQTRAIQYNQAIAALNRAKELCHLPDLTADSAAEWLETFQAK
                     ELEATEKMLSLEQKMSMAQTAHSQFEQAYQLVVAINGPLARNEAWDVARELLREGVDQ
                     RHLAEQVQPLRMRLSELEQRLREQQEAERLLADFCKRQGKNFDIDELEALHQELEARI
                     ASLSDSVSNAREERMALRQEQEQLQSRIQSLMQRAPVWLAAQNSLNQLSEQCGEEFTS
                     SQDVTEFLQQLLEREREAIVERDEVGARKNAVDEEIERLSQPGGSEDQRLNALAERFG
                     GVLLSEIYDDVSLEDAPYFSALYGPSRHAIVVPDLSQVTEHLEGLTDCPEDLYLIEGD
                     PQSFDDSVFSVDELEKAVVVKIADRQWRYSRFPEVPLFGRAARESRIESLHAEREVLS
                     ERFATLSFDVQKTQRLHQAFSRFIGSHLAVAFESDPEAEIRQLNSRRVELERALSNHE
                     NDNQQQRIQFEQAKEGVTALNRILPRLNLLADDSLADRVDEIRERLDEAQEAARFVQQ
                     FGNQLAKLEPIVSVLQSDPEQFEQLKEDYAYSQQMQRDARQQAFALTEVVQRRAHFSY
                     SDSAEMLSGNSDLNEKLRERLEQAEAERTRAREALRGHAAQLNQYNQVLASLKSSYDT
                     KKELLNDLQRELQDIGVRADSGAEERARIRRDELHAQLSNNRSRRNQLEKALTFCEAE
                     MDNLTRKLRKLERDYFEMREQVVTAKAGWCAVMRMVKDNGVERRLHRRELAYLSADDL
                     RSMSDKALGALRLAVADNEHLRDVLRMSEDPKRPERKIQFFVAVYQHLRERIRQDIIR
                     TDDPVEAIEQMEIELSRLTEELTSREQKLAISSRSVANIIRKTIQREQNRIRMLNQGL
                     QNVSFGQVNSVRLNVNVRETHAMLLDVLSEQHEQHQDLFNSNRLTFSEALAKLYQRLN
                     PQIDMGQRTPQTIGEELLDYRNYLEMEVEVNRGSDGWLRAESGALSTGEAIGTGMSIL
                     VMVVQSWEDESRRLRGKDISPCRLLFLDEAARLDARSIATLFELCERLQMQLIIAAPE
                     NISPEKGTTYKLVRKVFQNTEHVHVVGLRGFAPQLPETLPGSDEAPSQAS"
     gene            20817..22664
                     /gene="ldtD"
                     /locus_tag="NBI74_002530"
     CDS             20817..22664
                     /gene="ldtD"
                     /locus_tag="NBI74_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HCD1932849.1"
                     /translation="MLLNMMCGRRLSAISLCLAVTFAPLFNAQADEPEVIPGDSPVAV
                     SEQGEALPQAQATAIMAGIQPLPEGAAEKARTQIESQLPAGYKPVYLNQLQLLYAARD
                     MQPMWENRDAVKAFQQQLAEVAIAGFQPQFNKWVELLTDPGVNGMARDVVLSDAMMGY
                     LHFIANIPVKGTRWLYSSKPYALATPPLSVINQWQLALDKGQLPTFVAGLAPQHPQYA
                     AMHESLLALLSDTKPWPQLTGKATLRPGQWSNDVPALREILQRTGMLDGGPKITLPGD
                     DTPTDAVVSPSAVTVETAETKLMDKQTTSRSKPAPAVRAAYDNELVEAVKRFQAWQGL
                     GADGAIGPATRDWLNVTPAQRAGVLALNIQRLRLLPTELSTGIMVNIPAYSLVYYQNG
                     NQVLDSRVIVGRPDRKTPMMSSALNNVVVNPPWNVPPTLARKDILPKVRNDPGYLESH
                     GYTVMRGWNSREAIDPWQVDWSTITASNLPFRFQQAPGPRNSLGRYKFNMPSSEAIYL
                     HDTPNHNLFKRDTRALSSGCVRVNKASDLANMLLQDAGWNDKRISDALKQGDTRYVNI
                     RQSIPVNLYYLTAFVGADGRTQYRTDIYNYDLPARSSSQIVSKAEQLIR"
     gene            22845..23393
                     /locus_tag="NBI74_002531"
     CDS             22845..23393
                     /locus_tag="NBI74_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbK family protein"
                     /protein_id="HCD1932850.1"
                     /translation="MDKFDANRRKLLALGGVALGAAILPTPAFATLSTPRPRILTLNN
                     LHTGESIKAEFFDGRGYIQEELAKLNHFFRDYRANKIKSIDPGLFDQLYRLQGLLGTR
                     KPVQLISGYRSIDTNNELRARSRGVAKKSYHTKGQAMDFHIEGIALSNIRKAALSMRA
                     GGVGYYPRSNFVHIDTGPARHW"
     gene            23420..24067
                     /gene="gloC"
                     /locus_tag="NBI74_002532"
     CDS             23420..24067
                     /gene="gloC"
                     /locus_tag="NBI74_002532"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003035823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase GloC"
                     /protein_id="HCD1932851.1"
                     /translation="MNYRIIPVTAFSQNCSLIWCEQTRLAALVDPGGDAEKIKQEVDA
                     SGLTLMQILLTHGHLDHVGAAAELAQHYGVPVFGPEKEDEFWLQGLPAQSRMFGLEEC
                     QPLTPDRWLNEGDTISIGNVTLQVLHCPGHTPGHVVFFNEQARLLISGDVIFKGGVGR
                     SDFPRGDHNQLISSIKDKLLPLGDDVTFIPGHGPLSTLGYERLHNPFLQDEMPVW"
     gene            complement(24117..25307)
                     /gene="aspC"
                     /locus_tag="NBI74_002533"
     CDS             complement(24117..25307)
                     /gene="aspC"
                     /locus_tag="NBI74_002533"
                     /EC_number="2.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009653304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate transaminase"
                     /protein_id="HCD1932852.1"
                     /translation="MFENITAAPADPILGLADLFRADERPGKINLGIGVYKDETGKTP
                     VLTSVKKAEQYLLENETTKNYLGIDGIPEFGRCTQELLFGKGSALINDKRARTAQTPG
                     GTGALRVAADFLAKNTSVKRVWVSNPSWPNHKSVFNSAGLEVREYAYYDAENHTLDFD
                     ALINSLNEAQAGDVVLFHGCCHNPTGIDPTLEQWQTLAQLSVEKGWLPLFDFAYQGFA
                     RGLEEDAEGLRAFAALHKELIVASSYSKNFGLYNERVGACTLVAADSETVERAFSQMK
                     AAIRANYSNPPAHGASVVATILSNDALRAIWEQELTDMRQRIQRMRQLFVNTLQEKGA
                     NRDFSFIIKQNGMFSFSGLTKEQVLRLREEFGVYAVASGRVNVAGMTPDNMAPLCEAI
                     VAVL"
     gene            complement(25492..26580)
                     /gene="ompF"
                     /locus_tag="NBI74_002534"
     CDS             complement(25492..26580)
                     /gene="ompF"
                     /locus_tag="NBI74_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpF"
                     /protein_id="HCD1932853.1"
                     /translation="MMKRNILAVIVPALLVAGTANAAEIYNKDGNKVDLYGKAVGLHY
                     FSKGNGEDSYGGNGDMTYARLGFKGETQINSDLTGYGQWEYNFQGNNSEGADAQTGNK
                     TRLAFAGLKYADVGSFDYGRNYGVVYDALGYTDMLPEFGGDTAYSDDFFVGRVGGVAT
                     YRNSNFFGLVDGLNFAVQYLGKNERDTARRSNGDGVGGSISYEYEGFGIVGAYGAADR
                     TNLQEESPLGKGKKAEQWATGLKYDANNIYLAANYGETRNATPITNKFTNTSGFANKT
                     QDVLLVAQYQFDFGLRPSIAYTKSKAKDVDGIGDVDLVNYFEVGATYYFNKNMSTYVD
                     YIINQIDSDNKLGVGSDDTVAVGIVYQF"
BASE COUNT         6470 a         6521 c         7235 g         6445 t
ORIGIN      
        1 aatgtttata tataattaat caacggatga ttcacatgaa gaatactaaa ttattgctgg
       61 cgattgcgac ctctgcagcc ttactgacag gatgtcagaa tacgcacggt attaataccg
      121 atttggctat cagctcaggt ctgaatgctt ataaagccgc aacattaagc gatgctgatg
      181 ctaaagcaat cgccaatcag ggctgtgtcg aaatggacag cggcaataaa gtggcaagca
      241 aatccagcaa gtacgggaaa cgtctggcaa aaatcgccaa agcattgggt aacaatatta
      301 atggcacgcc agtcaactat aaggtttata tgaccagcga cgtcaacgca tgggcgatgg
      361 cgaacggttg tgttcgtgtc tacagtggcc tgatggacat gatgaacgac aacgaaattg
      421 aaggcgttct gggccatgaa ctgggccacg tcgcgttggg tcactcgctg gctgaaatga
      481 aagcttctta tgcgatcgtg gccgcacgcg atgccatttc agctaccagc ggtgtggctt
      541 cccagctttc ccgctcacaa ttgggtgata tcgcagaagg cgctatcaat gctaaatact
      601 cccgtgataa agagtcagaa gcagatgatt tctcctttga tctgttgaag aaacgtggca
      661 tcagtaccca ggggctggtt ggtagctttg aaaaactcgc tagcctggat ggcggtcaca
      721 cccagtccat gtttgattct cacccaccat caacagagcg tgcgcaacac atccgtgatc
      781 gtatcgcctc tggtaagtaa atcattgtca tctttcgggc tggtcttctg ccagcccgct
      841 ataattgcgc aataaatccc catctgaata cagacaaaac tggtttttgc acacaacgtt
      901 aacgatttgt ggcgtcggcg cgtataatgc gcgcggttat gttaacggta cgcctgtttt
      961 aaggagataa agatgacggc aattgccccg gttattacca ttgatggccc aagcggtgca
     1021 gggaaaggca ccttgtgtaa ggctatggcg gaagcgttgc aatggcatct gctggattcg
     1081 ggtgcaattt atcgcgtact ggcactagcg gcattacatc accatgttga tgttgcatcg
     1141 gaagatgcgc tggtgccgct ggcatcccat ctggatgtac gttttgtgtc aaccaacggc
     1201 aatctggaag tgatcctcga aggggaagat gtcagcggcg aaattcgtac tcaggaggtg
     1261 gcgaatgctg cttcacaagt cgcggcattc ccacgcgttc gtgaagcatt attgcgtcgc
     1321 caacgcgcgt ttcgcgaatt accaggcctg attgccgatg ggcgcgacat gggaacggtg
     1381 gtattccctg atgcgccagt gaaaattttc cttgacgcct cctcggaaga acgtgcgcat
     1441 cgccgcatgc tacagttgca ggagaagggc tttagtgtta actttgagcg ccttttggcc
     1501 gagatcaaag aacgtgacga ccgcgatcgt aatcgagcgg tagcgccact ggttccggca
     1561 gccgatgctt tagtgttgga ttccaccacc ttaagcattg agcaagtgat tgaaaaagcg
     1621 ctacaatacg cgcgccagaa attggctctc gcataagcga ccgaatttgc agtacccccg
     1681 ttgcaatgga atgacagcgg gtatgttaaa caaccccatc cggcatggag ccaggtggac
     1741 gttaaatata aacctgaaga ttaaacatga ctgaatcttt tgctcaactc tttgaagagt
     1801 ccttaaaaga aatcgaaacc cgcccgggtt ctatcgttcg tggcgttgtt gttgctatcg
     1861 acaaagacgt agtactggtt gacgctggtc tgaaatctga gtccgccatc ccggctgagc
     1921 agttcaaaaa cgcccagggc gagctggaaa tccaggtagg tgacgaagtt gacgttgctc
     1981 tggacgcagt agaagacggc ttcggtgaaa ctctgctgtc ccgtgagaaa gctaaacgtc
     2041 acgaagcctg gatcacgctg gaaaaagctt acgaagatgc tgaaactgtt accggtgtta
     2101 tcaacggcaa agttaagggc ggcttcactg ttgagctgaa cggtattcgt gcgttcctgc
     2161 caggttctct ggtagatgtt cgtccggtgc gtgacactct gcacctggaa ggcaaagagc
     2221 ttgaattcaa agtaatcaag ctggatcaga agcgcaacaa cgttgttgtt tctcgtcgtg
     2281 ccgttatcga atccgaaaac agcgcagagc gcgatcagct gctggaaaac ctgcaggaag
     2341 gcatggaagt taaaggtatc gttaagaacc tcactgacta cggtgcattc gttgatctgg
     2401 gcggcgttga cggcctgctg cacatcactg acatggcctg gaaacgcgtt aagcatccga
     2461 gcgaaatcgt caacgtgggc gacgaaatca ctgttaaagt gctgaagttc gaccgcgaac
     2521 gtacccgtgt atccctgggc ctgaaacagc tgggcgaaga tccgtgggta gctatcgcta
     2581 aacgttatcc ggaaggtacc aaactgactg gtcgcgtgac caacctgacc gactacggct
     2641 gcttcgttga aatcgaagaa ggcgttgaag gcctggtaca cgtttccgaa atggattgga
     2701 ccaacaaaaa catccacccg tccaaagttg ttaacgttgg cgatgtagtg gaagttatgg
     2761 ttctggatat cgacgaagaa cgtcgtcgta tctccctggg tctgaaacag tgcaaagcta
     2821 acccgtggca gcagttcgcg gaaacccaca acaagggcga ccgtgttgaa ggtaaaatca
     2881 agtctatcac tgacttcggt atcttcatcg gcctggacgg cggcatcgac ggcctggttc
     2941 acctgtctga catctcctgg aacgttgcag gcgaagaagc agttcgtgaa tacaaaaaag
     3001 gcgacgaaat cgctgcagtt gttctgcagg ttgacgcaga acgtgaacgt atctccctgg
     3061 gcgttaaaca gctcgcagaa gatccgttca acaactgggt tgctctgaac aagaaaggcg
     3121 ctatcgtaac cggtaaagta actgcagttg acgctaaagg cgcaaccgta gaactggctg
     3181 acggcgttga aggttacctg cgtgcttctg aagcatcccg tgaccgcgtt gaagacgcta
     3241 ccctggttct gagcgttggc gacgaagttg aagctaaatt caccggcgtt gatcgtaaaa
     3301 accgcgcaat cagcctgtct gttcgtgcga aagacgaagc tgacgagaaa gatgcaatcg
     3361 caactgttaa caaacaggaa gatgcaaact tctccaacaa cgcaatggct gaagctttca
     3421 aagcagctaa aggcgagtaa ttctctgact cttcgggatt tttattccga agtttgttga
     3481 gtttacttga cagattgcag gtttcgtcct gtaatcaagc actaagggcg gctacggccg
     3541 cccttaatca atgtagcaac agcagccgct taatttgcct ttaaggaacc ggaggaatca
     3601 tgaccaagtc agaattgata gaaagacttg ccacccagca atcgcacatt cccgccaaga
     3661 cggttgaaga tgcagtaaaa gagatgctgg agcatatggc ctcgactctt gcgcagggcg
     3721 agcgtattga aatccgcggt ttcggcagtt tctctttgca ctaccgcgca ccacgtaccg
     3781 gacgtaatcc gaagactggc gataaagtag aactggaagg aaaatacgtt cctcacttta
     3841 aaccaggtaa agaactgcgc gatcgcgcca atatttacgg ttaagttttt actcaaactt
     3901 gaacgcgaga aaagcacctg tcgggtgctt ttttcatttc tctaatctgg aactggaagc
     3961 tgcctcgcag agttttgagc agttttcacc ctatcgttaa attcttctga acgtgcctcg
     4021 gggaacgcaa aactcccacc agacaaccgt tcaacaaagt tgcacacttt ctataaacag
     4081 ggagggtgca tgaaaataac gacagtcggc gtatgcataa tttgcggaat ttttccgttg
     4141 ctgattttgc cccaattgcc agggacagta acccttgcgt ttctgactct cttcgcctgt
     4201 gtactggcat ttatccctgt taaaaccgtc cgttatatcg cgctgacgtt gctgtttttc
     4261 gtttggggca tattagcagc aaagcaaatt ttgtgggcag gagaaacctt aactggcgcg
     4321 acgcaggatg caattgttga gatcattgct actgacggca tgaccactca ttacggtcaa
     4381 attactcatc tacaaggtcg acgtatattc cctgcgccag gcctcgtact gtatggcgaa
     4441 tatcttccgc aagcggtttg tgccggacaa gtatggtcaa tgaaactcaa agttcgtgca
     4501 gttcatggtc aacttaatga tggcggcttt gatagccagc gttatgccat tgcccagcat
     4561 caaccgctca ccggccgttt tctgcaggca agtgtcattg aaccgaattg tagcctgcgt
     4621 gcacagtatc tggcgtcact acaaacaacg ctgcaaccct atatgtggaa cgcggttatt
     4681 cttggattag gtatggggga acggttatcc gtccccaaag aaatcaaaaa tatcatgcgt
     4741 gatactggaa cggcgcattt aatggcgata tcaggattgc acatcgcttt tgcggcgttg
     4801 ctggctgcag gactcattcg cggtgggcaa gtttttctgc ctgggtgctg gctccactgg
     4861 caaatgccat taattggtgg aatctgctgt gctgcttttt atgcctggct tacgggaatg
     4921 caacctcctg cattgcgtac cgtggtggcg cttgccattt ggggaatgct taagttaagt
     4981 ggacgacaat ggagtggctg ggatgtatgg atatgttgtc tggcggcaat tttgctgatg
     5041 gatcctgttg ccattctctc gcaaagttta tggctttctg ccgctgcggt cgcggcactg
     5101 attttttggt atcagtggtt tccctgtccg gagtggcagc tgccgccggt attgcgcgcg
     5161 gttgtttccc tcatccatct gcaactggga atcaccctcc tgcttatgcc cgtgcaaatt
     5221 gtcatatttc atggcattag tctgacctcg tttattgcaa atctttttgc aattcccctg
     5281 gtgacattta tcacggttcc gttgattctc gccgcgatgg ttgtgcattt aagcgggccg
     5341 tttattctgg aacaagggtt atggtttctt gccgaccggt ctttggcttt acttttctgg
     5401 gggttaaaga gtttgccgga agggtggatc aacattgctg aacgttggca atggctaaca
     5461 ttttccccat ggttcttact ggtggtatgg cgattaaacg cctggcgaac gttgccagca
     5521 atgtgtgtgg ctgtaggctt gctgatgtgc ttgccgctgt ggcaaaaacc tcgacctgac
     5581 gagtggcaag tgtacatgct tgatgtcggg caggggctgg caatggtcat tgcgcgaaac
     5641 ggcaaagcga ttctctatga cacgggactg gcctggcccg aaggggatag tgggcaacaa
     5701 ctgattatcc cctggctcca ctggcataat cttgaaccgg aaggcgttat tttgagtcat
     5761 gaacatctgg atcaccgggg agggctggac tcaatattgc acacatggcc gatgttatgg
     5821 atccgaagtc cgttaaactg ggaacatcat cagccctgtg tgcgtggcga agcgtggcaa
     5881 tggcaaggat tgcgtttcag cgcgcactgg cctttacaag gtagcaacga taaaggaaat
     5941 aaccattcct gtgtggttaa gattgatgac gggacgaata gcattcttct aaccggtgat
     6001 attgaagccc ccgctgaaca aaagatgcta agccgttact ggcagcaaat gcaggcaaca
     6061 ttacttcagg tacctcacca tggcagtaat acctcatcat cgttgccatt aattcagcga
     6121 gtgaatggaa aagtggcact cgcatcggca tcgcgctata acgcatggcg actgccctca
     6181 agcaaagtta aatatcgcta tcatcaacag ggttataagt ggcttgatac tccacatcag
     6241 gggcaaataa cggtcaattt ttcagcgcaa ggctggtgga ttagcagcct cagagagcaa
     6301 attttacctc gttggtatca tcagtggttt ggcgtgccag tggataacgg gtagaatatg
     6361 cggctatttc aacaaatgct ggttttttga atgcataacg acaaagatct ctctacgtgg
     6421 cagacgttcc gccgactgtg gccaaccatt gcgcctttta aagcgggtct gatcgtggcg
     6481 ggcgtagcgt taatcctcaa cgcagccagc gataccttca tgttatcgct ccttaagcca
     6541 cttcttgatg atggctttgg taaaacagat cgctccgtgc tgatgtggat gccgctggtg
     6601 gtgatcgggc tgatgatttt acgtggtatc accagctata tctccagcta ctgtatctcc
     6661 tgggtatcag gaaaggtggt aatgactatg cgtcgccgcc tgtttggtca catgatggga
     6721 atgccggttt cattctttga caaacagtca acgggtacgc tgttgtcacg tatcacctac
     6781 gattccgaac aggtcgcttc ttcttcttcc ggcgcactga ttactgttgt gcgtgaaggt
     6841 gcgtcgatca tcggcctgtt catcatgatg ttctattaca gttggcaact gtcgatcatt
     6901 ttgattgtgc tggcaccgat tgtttcgatt gcgattcgcg ttgtatcgaa gcgttttcgc
     6961 aacatcagta aaaacatgca gaacaccatg gggcaggtga ccaccagcgc cgaacaaatg
     7021 ctgaaaggcc ataaagaagt attgattttc ggtggtcagg aagtggaaac gaaacgcttc
     7081 gataaagtca gcaaccgaat gcgtcttcag gggatgaaaa tggtttcagc ctcttccatc
     7141 tctgatccga tcattcagct gatcgcctct ttggcgctgg cgtttgttct gtatgcggca
     7201 agcttcccaa gtgtcatgga tagcctgact gccggtacga ttaccgttgt tttctcttca
     7261 atgattgcac ttatgcgtcc gctgaaatcg ctgaccaacg ttaacgccca gttccagcgc
     7321 ggtatggcgg cttgtcagac gctgtttacc attctggaca gtgagcagga gaaagacgaa
     7381 ggtaagcgcg tgatcgagcg tgcgactggc gacgtggagt ttcgcaatgt cacctttact
     7441 tatccgggac gtgatgtgcc tgcattgcgt aacatcaacc tgaaaattcc ggcagggaag
     7501 acggttgctc tggttggccg ctctggttca ggtaaatcaa ccatcgccag cctaatcaca
     7561 cgtttttacg atattgatga aggcgaaatc ctgatggatg gtcacgatct gcgcgagtat
     7621 accctggcgt cgttacgtaa ccaggttgct ctggtgtcgc agaatgtcca tctgtttaac
     7681 gatacggttg ccaacaacat tgcttacgca aggactgaac agtacagccg tgagcaaatt
     7741 gaagaagctg cgcgtatggc ctacgccatg gacttcatca ataagatgga taacggtctc
     7801 gatacagtca ttggtgaaaa cggcgtgttg ctctctggcg gtcagcgtca gcgtattgct
     7861 atcgctcgag ccttgttgcg tgatagcccg atactgattc tggacgaagc tacctcggcg
     7921 ttggataccg aatccgaacg tgcgattcag gcagcactgg atgagttgca gaaaaaccgt
     7981 acctctctgg tgattgccca ccgcttgtct accattgaaa aggcagacga aatcgtggtt
     8041 gtcgaggatg gtgtcattgt ggaacgcggt acgcataacg atttgcttga gcaccgtggc
     8101 gtttacgcgc aacttcacaa aatgcagttt ggccaatgat cgaaaaaatc tggtctggtg
     8161 agtccccttt atggcggcta ttgctgccac tctcctggtt gtatggcctg gtgagtggcg
     8221 cgatccgtct ttgctataaa ctaaaactga agcgcgcctg gcgtgccccc gtaccggttg
     8281 tcgtggttgg aaatctcact gcaggcggca acggaaaaac cccggtcgtt gtctggctgg
     8341 tggaacagct gcaacagcgc ggtattcgcg tgggggtcgt atcgcgggga tatggtggta
     8401 aggctgaatc ttatccgctg ttattgtcgg cagataccac cacagcacag gcgggtgatg
     8461 agcctgtgtt gatttatcaa cgcactggcg cgcctgttgc ggtttctccc gtgcgttctg
     8521 acgcggtaaa agccattctg gcgcaacacc ctgatgtgca gatcatcgta actgacgatg
     8581 gtttacagca ttatcgtctg gcgcgtgatg tggaaattgt cgttattgat ggtgtgcgtc
     8641 gctttggcaa tggctggtgg ttgccggcgg ggccaatgcg tgagcgagca gggcgcttaa
     8701 agttggttga tgcggtaatc gtcaacggcg gtgtcccccg cagcggtgaa atccccatgc
     8761 atctgctgcc gggtcaggcg gtgaatttac gtaccggtac acgttgtgac gttgctcagc
     8821 ttgaacatgt ggtggcgatt gcggggattg ggcatccgcc gcgctttttt gccacgctga
     8881 agatgtgtgg cgtacaaccc gaaaaatgtg taccgctggc cgatcatcag tttttgaatc
     8941 atgcggatgt cagcgcgttg gtaagcgccg gacaaacgct ggtaatgact gaaaaagatg
     9001 cggtgaaatg ccgggccttt gcagaagaaa attggtggta tttgcctgta gacgcacagc
     9061 tttcaggtga tgaaccagcg aaactgcttg cgcaactaac ttcgctggct tctggcaact
     9121 agttacgccg cggcagcgtt cgattgatgg agtcatgaat gtctctgccg cacctctccc
     9181 ttgctgatgc gcgtaatctt catctcgctg cacaaggcct gttaaacaaa ccccaccgtc
     9241 gagcgtcgtt ggaggatatt ccggcaacga tctcccgcat gtccttgctg caaatcgata
     9301 ccatcaatat tgttgcccgt agcccatatc tggtgctttt cagtcgtctg ggaaattatc
     9361 ctgcccagtg gctggatgag tctctggcgc gtggcgaatt aatggaatac tgggcgcatg
     9421 aagcctgctt tatgccgcgt agcgattttc gtcttattcg ccaccgcatg ctggcacctg
     9481 aaaaaatggg ctggaaatat aaagacgcct ggatgcagga acatgcggcg gaaattgcac
     9541 tgttaattca gcatattcat gatagggggc cggtacgttc agccgatttt gagcatcctc
     9601 gtaaaggtgc aagcggctgg tgggagtgga agccgcataa acgacatctg gaaggtttat
     9661 ttactgccgg aaaggtgatg gtgattgaac ggcgcaactt ccagcgcgtt tatgatttaa
     9721 cccaccgtgt catgcctgac tgggatgatg agcgcgatct cgtttcgcaa gcagaagcag
     9781 aaatcatcat gctggataac agtgcgcgta gcctgggaat atttcgcgaa cagtggctgg
     9841 cagattacta ccggctaaaa cgtccggcac tggcggcgtg gcgtgaagcg agggctgaac
     9901 agcagcaaat cattgctgtg catgttgaaa aattgggcaa tctttggctg catgctgatt
     9961 tgctgccgct actcgagcgt gcgctcgccg gaaagctcac tgcaacgcac agcgcggtac
    10021 tttcgccttt tgatcctgtt gtctgggatc gcaaacgcgc ggagcagctt tttgatttta
    10081 gctaccggct ggagtgctat accccagcgc cgaaacgcca gtatggctat tttgttctgc
    10141 cgttattaca tcgtgggcaa ttagttgggc gaatggattc caaaatgcat cgccagaaag
    10201 gcatccttga agttatctct ctgtggttac aggaaggcat taaaccaacg acaacgctgc
    10261 aaaaagggtt acgtcaggcg attactgatt tcgctaactg gcagcaggca acgcgggtga
    10321 cattaggacg ctgctcgcaa ggcctcttta ctgattgccg cgccggctgg gaaatagacc
    10381 ccgtcgcata aatgattatg ataagattta tgcattcata acccgatcca tctggaggaa
    10441 ctatggatca tcgtctgctt gaaatcattg cctgcccggt ttgcaacgga aaactttggt
    10501 ataaccagga aaaacaagag cttatttgca aactggacaa cctggctttc ccgctgcgcg
    10561 atggcattcc agtgttgctg gaaacggaag cccgcgtgct gactgctgat gagggtaaat
    10621 catgagtttt gtggtcatta ttcccgcgcg ttacgcgtcc acgcgtctgc ccggtaaacc
    10681 attggttgat attaacggca aacccatgat tgttcatgtt cttgaacgcg cgcgcgaatc
    10741 aggtgccgag cgcatcatcg tggcaaccga tcatgaggat gttgcccgcg ctgttgaagc
    10801 cgctggcggc gaagtatgta tgacgcgcgc cgatcatcag tcaggtacag aacgtttggc
    10861 ggaagttgtc gaaaaatgcg cattcagcga cgacacggtg atcgttaatg tgcagggtga
    10921 tgaaccgatg atccctgcga caatcattcg tcaggttgct gataacctcg ctcagcgtca
    10981 ggtgggtatg gcgactctgg cggtgccaat ccacaatgcg gaagaagcgt ttaacccgaa
    11041 tgcggtgaaa gtggttctcg atgccgaagg gtacgcactg tacttctctc gcgccaccat
    11101 tccgtgggat cgtgatcgtt ttgcaaaaga ccttgaaacc gttggcgata acttcctgcg
    11161 tcatcttggt atttatggct accgagcagg ctttatccgt cgttatgtta cctggcagcc
    11221 aagtccgtta gaacatatcg aaatgttaga gcagcttcgt gttctgtggt atggcgaaaa
    11281 aatccacgtt gctgtcgccc aggaagttcc tggcacaggt gtggataccc ctgaagatct
    11341 cgagcgcgtt cgcgctgaaa tgcgctaatt tcacttcacg acactccagc caattttggg
    11401 aggagtgtcg taccgttacg attttcctca atttttcttt tcaacaattg atctcattca
    11461 ggtgacatct tttatattgg cgctcattat gaaagcagta gcttttatga gggtaatctg
    11521 aatggaacag ctgcgtgccg aattaagcca tttactgggc gaaaaactca gtcgtattga
    11581 gtgcgtcaat gaaaaagcgg atacggcgtt gtgggctttg tatgacagcc agggaaaccc
    11641 aatgccgtta atggcaagaa gctttagtac gcccggaaaa gcccgacaac tggcatggaa
    11701 aaccaccatg ctggcaagaa gtgggactgt ccgtatgccg actatttatg gtgtgatgac
    11761 gcatgaagaa caccccggcc ccgatgtcct gctactggag cgaatgcgtg gtgtttcggt
    11821 ggaggcacca gcccgaacac cagaacgctg ggaacaactc aaagaccaaa tcgttgaagc
    11881 cttactggcc tggcaccgtc aggacagtcg tggttgcgtc ggcgcggtcg acaatactca
    11941 ggaaaatttc tggccctcat ggtaccggca acatgttgaa gtgctatgga ccacgctcaa
    12001 tcagttcaat aacaccggtc tatcgatgca ggataagcgg atcctgtttc gcactcgcga
    12061 atgtctcccg gcattatttg aaggctttaa cgacaattgt gtactgattc acggtaactt
    12121 ctgtttacgc agcatgctga aagattcgcg cagcgatcag ttactggcga tggtcggacc
    12181 gggactaatg ctttgggcac cgcgagaata cgaactgttc cgactaatgg ataattctct
    12241 ggcggaagat ttgctctgga gttatctgca acgcgcgcca gtggcggagt cgttcatctg
    12301 gcggcgttgg ttgtatgtgt tatgggatga agttgcgcaa ctggtcaaca ccggacgatt
    12361 taatcggcgt aacttcgatc tggcatcaaa atcactcttg ccgtggctcg cctgacgaac
    12421 ctttcagcca ctgccagatc cggccaagcg tttcatagcc aacgcggtcg ctatgcatca
    12481 accaaaccgg agaagggatt gcccgttccc acggattaag cggcgagtcg atggccaact
    12541 gattcgccgg ggctggtagc ggatttaacc cttcctgctg aaaaaagatc atcgcccgcg
    12601 gcagatgtga tgcggaggtc accagcagga aaggtgcgtc accaatcgcc tgtttcactg
    12661 ctgcagcttc ttcttcggta tcttttggca aatccagggt gataatttgc tcgcgcggca
    12721 cgcccagcga ttgcgcaact ctggcaccga cttcggctgt gctcaccgta ttggttttag
    12781 ctacgcctcc cgtgaagatc agttttgatc ccggattttc gcgccataag cgaatacctt
    12841 cattcaggcg tggcagactg ttattgatta aattagagct cggcgcccac tgcgggttcc
    12901 aggtataacc acctccgagc accacgatat agtccacttt ttgcgaatta ttccatgtcg
    12961 gataggtgct ttcgatggga cgcagcaggc ggtcggctac cggttgcaaa ctcaataaca
    13021 agagcgccag ccacccaatg ctaataaaaa tcttcccggt tttttgaaaa cggctaaacc
    13081 acagcagcgc caggccagcg ccaataatca acaacattag cggaagtggc agcaacatat
    13141 tgccaatcac ttttttcagt gtaaaaagca tcctttctgg ttcctttttt aaccatatag
    13201 caagggatcg ccagcgatat tacaccagac gggttcattc tcgccgtggc tgtgacaaaa
    13261 tagcgtcttt atcagaaaac gtcttagcca accggtggag aaagcaatgc aggatcgcaa
    13321 ttttgatgat attgcggaaa agttttcccg taacatttac ggcaccacca aagggcagct
    13381 tcgacaggct attctgtggc aggatctcga tcgcgtgctg gcagaaatag gcccgcaaaa
    13441 actgcgtgtg ctggatgctg gcggtggaga agggcagacc gcaatcaaaa tggccgagcg
    13501 cgggcatcag gtcattttat gcgatctttc tgcgcagatg atcgaccgcg caaaacaggc
    13561 ggcagaagca aaaggtgtga gcgacaacat gcaatttata cattgcgccg ctcaggatgt
    13621 tgcttcgcat ttggaaacgc ccgttgatct gatattgttc catgcggtgc tcgagtgggt
    13681 ggccgatccc cgcagcgtat tacagaccct gtggtcagtg ttgcgtccag gcggcgtgtt
    13741 gtcgttaatg ttctacaatg cgcatggttt gttgatgcat aacatggtcg ccgggaattt
    13801 tgattacgtg caggcgggaa tgccgaaaaa gaaaaaacgg acgctttcgc cagattatcc
    13861 acgcgacccg gcgcaggttt atctgtggct ggaagaagct ggttggcaaa ttctgggtaa
    13921 gacaggcgtt cgcgtgtttc atgattatct gcgtgagaag caccagcagc gcgactgcta
    13981 tgaagcatta cttgaattag aaacgcgtta ttgccgtcag gaaccgtata ttaccctggg
    14041 gcgttatatt catgtcaccg cgcgcaaacc gcagagcaag gataaagtat gagtgaattt
    14101 tcccagacag tccccgaact ggttgcctgg gccagaaaaa atgacttctc catctcgctg
    14161 ccggtagacc gactctcttt tctgctggcg gttgccacgc tgaacggcga gcgtctggat
    14221 ggtgagatga gtgaaggcga gctggtggat gcattccgcc atgtgagtga tgcgtttgag
    14281 caaaccagcg aaaccatcgg cgtgcgcgcc aacaacgcga tcaacgacat ggtgcgtcaa
    14341 cgtctgctga accgctttac cagcgagcag gcggaaggga acgcaattta ccgtctgacg
    14401 ccgctcggca tcggcatcac tgactattac attcgtcagc gcgagttttc tacgctgcgc
    14461 ctttctatgc aattgtcgat tgtggcgggt gagctcaaac gcgcagcaga tgccgccgaa
    14521 gagggcggtg atgaatttca ctggcaccgc aatgtttatg cgccactgaa atattcggta
    14581 gcagaaattt tcgacagtat cgacctgacg caacgtctga tggatgaaca gcagcagcag
    14641 gtgaaggatg atatcgccca gttgttgaac aaagactggc gggcggcgat ttccagctgt
    14701 gaattgttgc tttcagaaac ttccggaacg ctgcgtgaat tgcaggatac gctggaagcg
    14761 gcaggcgata aattgcaggc caacctgtta cgcattcagg atgcgacgat gacccatgac
    14821 gacctgcatt ttgtcgatcg tctggtgttc gatcttcaga gcaaactcga ccgtattatc
    14881 agttggggcc agcaatccat cgacttgtgg attggctacg accgccacgt acacaaattt
    14941 attcgtactg cgatcgatat ggataaaaac cgcgtctttg ctcagcggtt acgtcagtcg
    15001 gtacaaacct attttgatga gccgtgggcg ctaacttatg ccaatgccga tcgtctgctg
    15061 gatatgcgtg acgaagagat ggtactgcgc gatgaagaag tgactgggga acttcctgag
    15121 gatctggagt acgaagagtt taacgagatc cgcgaacagc tggcggcgat catcgaagaa
    15181 caacttgccg tgtacaaaac cagacaagtg ccgttggatc ttggtctggt ggtacgcgaa
    15241 tatctgtcac agtatccgcg tgcacgtcac tttgacgttg cgcgcattgt tattgatcag
    15301 gcggtacgtc ttggcgtagc gcaagcagat ttcaccggac tgccagcgaa atggcagccg
    15361 attaatgatt acggagccaa ggtacaggcg catgtcatcg acaaatattg aacaagtgat
    15421 gccggttaag ctggcgcagg cgctggcgaa tccgttattt ccggcgctgg atagcgcctt
    15481 acgttcaggc cgccatattg gcctcgacga actggataat catgcattcc tgatggattt
    15541 tcaggaatat ctggaagagt tttacgcgcg ttataacgtt gagcttattc gtgcaccgga
    15601 agggttcttc tatctacgcc cacgttccac cacgctgatc cctcgttccg tcttgtcgga
    15661 actggatatg atggtcggga aaatcctctg ttatctctat ctcagcccgg aacggctggc
    15721 gaatgagggg atttttactc aacaggaact gtacgacgaa ctgctcaccc tggccgatga
    15781 agcgaaactg ctgaaactgg tgaacaaccg ttcaaccggt tcagacgttg accgtcagaa
    15841 gttgcaggaa aaagtacgtt cttcgctcaa ccgtctgcgt cgtttaggca tggtgtggtt
    15901 tatgggccac gacagcagca agttccgcat taccgaatcg gtgttccgct tcggcgccga
    15961 tgtgcgtgct ggcgacgatc cccgtgaagc acagcgtcgc ctgattcgtg atggtgaagc
    16021 aatgccgatt gaaaatcatc tgcaactcaa cgatgaaacc gaagagagtc agccagatag
    16081 cggagaggaa gaataatgat tgaacgcggt aaatttcgct cactgacgct gattaactgg
    16141 aacggctttt ttgcccgaac ttttgacctt gacgagctgg tcacgacgct ttctggcggt
    16201 aacggggcgg gtaaatccac caccatggcg gcgttcgtta cggcgcttat ccccgatctg
    16261 accctgctgc atttccgtaa cactacggaa gccggggcca ccagcggttc gcgcgataaa
    16321 ggtctgcacg gtaagctgaa agcgggtgtc tgttattcga tgctcgacac cattaactcg
    16381 cgtcaccagc gcgtggtggt cggtgtgcgt ctgcaacagg ttgccggacg cgatcgtaaa
    16441 gtggatatca agccgtttgc cattcaggga ctgccgatgt cggtgcagcc gacacaactg
    16501 gtgaccgaaa ctctgaacga acgccaggcg cgcgtgctgc cgcttaacga gctgaaagac
    16561 aagctcgagg cgatggaagg cgtgcagttt aaacagttca actccattac cgattaccac
    16621 tcgctgatgt tcgatctggg catcatcgcg cgtcgtctgc gctcagcgtc tgaccgtagt
    16681 aaattctatc gtctgatcga agcctcgctg tatggcggga tctccagcgc cattactcgt
    16741 tctctgcgcg actacctgtt gccggaaaac agtggggtgc ggaaagcgtt ccaggacatg
    16801 gaagcggcgc tgcgtgaaaa ccgcatgacg ctggaagcga ttcgtgtcac ccagtcggac
    16861 cgtgacctgt ttaagcatct gatcagcgaa gccaccaact acgtggcggc ggactacatg
    16921 cgtcacgcca acgagcgccg tgttcatctc gataaggccc tggagtttcg tcgcgagcta
    16981 catacttcgc gtcagcaact ggcggctgag cagtacaaac acgtcgacat ggcgcgagag
    17041 ctggctgagc acaacggtgc cgaaggcgat ctggaagcgg attatcaggc ggccagcgat
    17101 cacctgaacc tggtgcaaac cgcactgcgt cagcaggaga agatcgaacg ctacgaagcg
    17161 gatctcgatg agctgcagat ccgtctggaa gagcaaaacg aagtggtggc agaagccatc
    17221 gaacgccagg aagagaacga ggctcgtgcg gaagctgccg aactggaagt ggacgagctg
    17281 aaaagccagc ttgctgacta ccagcaggcg ctggacgtcc agcaaacgcg cgcgatccag
    17341 tataaccagg cgattgctgc gcttaatcgt gccaaagaac tgtgccatct gccggactta
    17401 accgccgaca gcgctgccga atggctggaa accttccagg cgaaagagct ggaagcgacc
    17461 gaaaaaatgc tctctcttga gcagaaaatg agcatggcgc aaaccgcgca cagccagttt
    17521 gagcaggctt atcagctggt ggtggcaatc aacggcccgc tggcgcgtaa cgaggcgtgg
    17581 gatgtcgctc gcgaactatt gcgcgaaggg gtcgatcagc gtcacctggc agagcaggtt
    17641 cagccgttgc ggatgcgctt aagcgaactg gaacagcgtc tgcgtgagca gcaagaagct
    17701 gagcgtctgc tggcagattt ctgcaaacgt cagggcaaga attttgatat tgatgaactg
    17761 gaagctctgc atcaggagct ggaagcacgc attgcctctc tttccgatag cgtctctaac
    17821 gcccgagaag agcggatggc actgcgccag gagcaggaac agctgcagtc tcgcattcag
    17881 agtttgatgc agcgtgcgcc ggtttggctg gcagcgcaaa acagtctcaa ccagttgagc
    17941 gaacagtgcg gcgaagagtt cacctccagc caggatgtca ccgaatttct gcaacagttg
    18001 ctggagcgtg agcgcgaggc aattgttgaa cgcgatgaag taggcgcgcg caaaaacgcc
    18061 gtcgatgaag agatcgaacg tttaagccag ccaggtggtt ctgaagatca gcgtctgaac
    18121 gcgctggcgg agcgttttgg tggtgtgctg ctgtcagaaa tttatgacga cgttagcctg
    18181 gaagatgcgc cgtacttctc agcactgtat ggcccgtcac gccacgccat cgtggtgcca
    18241 gatctgtcac aggtaaccga acacctggaa ggcttgaccg attgcccgga agatctctat
    18301 ttgatcgaag gggatccgca gtcattcgat gacagcgtgt tcagcgttga tgagctggaa
    18361 aaagcggtgg tggtgaaaat cgccgatcgt cagtggcgtt attcacgttt cccggaagtg
    18421 ccgctgtttg gtcgtgctgc gcgtgaaagc cgtattgaaa gcctccatgc cgagcgggaa
    18481 gtgctttccg aacgcttcgc cacgctttcc tttgatgtac agaaaaccca gcgtctgcat
    18541 caggcgttca gccgctttat cggcagtcat ctggcggtgg cgtttgagtc tgacccggaa
    18601 gcagaaatcc gtcaactgaa cagccgtcgc gtcgaactgg aacgggcgtt gagtaatcat
    18661 gaaaatgata accagcagca gcgtattcag tttgagcagg cgaaagaggg cgttacggcg
    18721 ctgaaccgca ttctgccgcg tctcaacctg ttggctgatg acagcctggc ggatcgcgtc
    18781 gatgaaatcc gcgaacgtct ggatgaagcc caggaagccg cgcgttttgt tcagcagttt
    18841 ggcaatcaac tggcgaaact ggagccgatc gtttcggtat tgcagagcga cccggaacag
    18901 ttcgaacaat taaaagaaga ttacgcgtac tctcagcaga tgcagcgcga tgcccgtcag
    18961 caggcgtttg ccctgacgga agtggtgcag cgtcgtgcgc actttagcta ctctgactcg
    19021 gcagaaatgc ttagcggtaa cagcgatctc aacgaaaaac tgcgtgaacg tctggaacag
    19081 gcggaagcgg agcgtacccg cgctcgtgaa gcgttgcgcg gacacgcagc gcagttgaat
    19141 cagtacaacc aggtgctggc ttcgctgaaa agttcgtatg acaccaaaaa agagctactt
    19201 aacgatctgc aacgtgaatt gcaggatatc ggcgtgcgtg ctgatagcgg ggcagaagag
    19261 cgggcacgta ttcgccgtga cgagttgcac gcgcaactga gcaataaccg ttcacgacgc
    19321 aatcaactgg aaaaagcgct taccttctgc gaagcggaga tggacaacct gacccgtaaa
    19381 ctgcgcaagc tggagcggga ttactttgag atgcgcgagc aggtagtgac cgccaaagcg
    19441 ggctggtgtg cggtgatgcg catggtgaaa gataatggcg ttgagcgtcg cttacaccgt
    19501 cgtgagctgg cttatctctc cgctgatgat ttgcgctcca tgtcggataa ggcgttaggt
    19561 gcgctgcgtc tggcggtggc ggataacgaa catctgcgcg acgtgctgcg gatgtcggaa
    19621 gatccaaaac gtccggaacg taaaattcag ttcttcgtgg cggtttatca gcatctgcgt
    19681 gaacgtattc gtcaggatat tattcgtacc gatgatccgg tggaagctat cgaacagatg
    19741 gagatcgagc ttagccgtct gaccgaagaa ttaacctctc gtgaacagaa actggcgatc
    19801 agttcccgca gcgtggcgaa catcattcgc aaaaccattc agcgcgagca gaaccgtatc
    19861 cgtatgctca accaggggtt gcagaacgta tcgtttggcc aggtgaacag cgtacgtctg
    19921 aacgtgaacg tacgtgaaac gcacgccatg ctgctggatg tgctctctga acagcacgag
    19981 cagcatcagg atctgtttaa cagcaaccgc ctgaccttct cagaagcgct ggcgaaactg
    20041 tatcaacgtc ttaacccgca gattgatatg gggcagcgca cgccgcagac catcggtgaa
    20101 gaactgctgg attaccgcaa ctatctggaa atggaagttg aggttaaccg tggttccgat
    20161 ggctggttgc gtgcagagtc tggtgcattg tcgaccggtg aggcgattgg taccggtatg
    20221 tcgattctgg tgatggtggt acaaagctgg gaagatgaat ctcgccgcct gcgcggtaaa
    20281 gatatctctc cttgccgcct gctgttcctc gatgaagcag cgcgactgga cgcccgttcc
    20341 atcgccacgc tgtttgaatt gtgtgaacgt ttgcaaatgc agctcattat cgcagcgccg
    20401 gaaaatatca gcccggagaa aggcaccacc tataaactgg tacgtaaagt cttccagaat
    20461 accgaacacg ttcacgtcgt cggcctgcga ggatttgcgc ctcaactccc tgaaacgctt
    20521 ccaggaagtg acgaagcgcc ttctcaggcg agttaaaatt aaggcggcag caatgccgcc
    20581 ttttcttttt ccgaaaactc cgtttctgca ctaaaaaagt ggcacattac ggcgcgcttt
    20641 tctttaaact tctttacatt aggttatgta aaaacgtatc ggcgtttata tactgaagat
    20701 aagcctgatg agtaacaggc ttgctcgtca tactttcgtg agtattggcg ttgtacaggc
    20761 aagtcgtaaa ataacagcct ggctattcag agtatgataa aaacaggggg caagggatgt
    20821 tgcttaatat gatgtgtggt cgtcggctgt cggcaatcag tttgtgcctg gccgtaacat
    20881 tcgctccact gttcaatgcg caggccgatg agcctgaagt aatccctggc gacagcccgg
    20941 tggctgtcag tgaacagggc gaggcactgc cgcaggcgca agccacggca ataatggcgg
    21001 ggatccagcc attgcctgaa ggtgcggctg aaaaagcccg cacgcaaatc gaatctcaat
    21061 tacccgcagg ttataagccg gtttatctta accagcttca attgttgtat gccgcacgcg
    21121 atatgcaacc catgtgggaa aaccgtgatg ctgttaaagc cttccagcaa cagctggcag
    21181 aggtggcgat tgccggtttc cagccgcagt ttaataaatg ggtagagtta ctgaccgatc
    21241 ctggtgttaa cgggatggca cgcgacgtgg tgctctctga tgcgatgatg ggctatctcc
    21301 atttcattgc aaatattccg gtcaaaggca ctcgctggct atatagcagt aaaccttatg
    21361 cgcttgcaac gccgccgctt tcggtgatta accaatggca gctggcgctg gataaaggtc
    21421 aattgccgac gtttgttgca ggactggcac cgcagcatcc gcaatatgcg gcgatgcatg
    21481 aatcgttact ggccttactc agtgacacca aaccgtggcc tcaactgacc ggtaaagcaa
    21541 cgttgcgccc aggacagtgg agtaacgacg taccggcgtt gcgcgaaata ttgcaacgta
    21601 caggcatgtt ggacggggga ccgaaaatta cgctacctgg cgatgacacg ccaactgacg
    21661 cggtagtcag cccatcggct gttactgttg aaacagcaga aactaagctg atggacaaac
    21721 aaactacgtc tcgtagtaaa cctgcgcctg ccgttcgcgc cgcctacgat aatgaactgg
    21781 tggaagccgt taaacgtttt caggcatggc aaggattggg agcagatggt gctattggcc
    21841 cggcaacgcg tgactggtta aacgtaacgc ccgcccagcg tgctggcgtg ttggcactca
    21901 acatccagcg attgcgcttg ctgccaacag agctttctac cgggatcatg gttaacattc
    21961 cagcctattc gctggtctac tatcagaacg gcaatcaggt gctggattcg cgagtcattg
    22021 tcggtcgccc cgatcgcaaa acgccgatga tgagcagtgc acttaacaac gtagtggtaa
    22081 acccgccgtg gaacgtacca ccaactctgg cacgcaaaga tattctgccg aaagtgcgca
    22141 acgatccggg atatctcgaa agccatggct atacggtgat gcgcggctgg aacagcagag
    22201 aagcgattga cccatggcag gttgactggt ctacaatcac ggcctcgaat ttaccgttcc
    22261 gtttccagca ggctccaggc ccacggaact cgctggggcg ctataaattc aatatgccga
    22321 gttcagaggc catttatttg catgacacgc cgaaccacaa tctgttcaaa cgtgatacac
    22381 gcgcattgag ctcaggctgt gtacgagtga ataaagcttc cgatctggcg aatatgctgt
    22441 tgcaggatgc aggatggaat gacaaacgta tttctgatgc gctgaagcag ggcgatacgc
    22501 gttacgtcaa tattcggcag tcgattccgg tgaatctcta ctatctgacg gcctttgttg
    22561 gtgctgatgg tcgtacccag tatcgtacag atatttacaa ttatgatctg cctgcgcgat
    22621 ccagctcgca aattgtatcg aaagcggaac aattaatcag gtaaatgaag cagttcgggg
    22681 aaatcagttg tcgtaataat tagcatgatc gggggcgatt ctctgcagcc cccgtcactg
    22741 ctggggttga gtcatcttga cgtcttcttt acgggcggtt aaggtgcctc ttgtgcgcca
    22801 gaagtgcata taaacgataa cattgacctg tagacttgat tatcatggac aaattcgacg
    22861 ctaatcgccg caaactgctg gcgcttggtg gcgttgcact cggtgccgcc atcctgccga
    22921 ccccggcgtt tgcaacactc tctaccccac gcccgcgcat tttgacactc aataatcttc
    22981 ataccggaga gtcaatcaaa gcggagtttt tcgatggcag aggctatatt caggaagaat
    23041 tggcaaaact taaccatttt ttccgcgatt accgcgcgaa caaaataaag tccatcgacc
    23101 caggattatt cgaccagttg tatcgcctgc aagggttgtt aggcacccgc aaaccggtgc
    23161 aactcatttc cggttatcgt tccattgata ccaacaatga actacgcgcc cgcagccgtg
    23221 gagtagcgaa gaaaagctat cacaccaaag gccaggcgat ggatttccac attgaaggta
    23281 tcgcgttaag caatattcgc aaagccgcgt tatctatgcg cgcaggtggt gtaggatatt
    23341 acccacgtag taactttgtg catattgata ccgggccagc acggcactgg tagtaatcgc
    23401 ttaacgaaac aggggcagta tgaactatcg tattattccg gtcaccgcat tctcccagaa
    23461 ctgttcattg atctggtgtg aacaaacccg tctggccgca ctggtcgatc ctggcggcga
    23521 tgcggaaaaa atcaaacagg aagttgatgc cagcggcctg acactgatgc agatcctgct
    23581 gactcatggt catctggacc acgttggcgc agcggcggaa ctggcgcaac attacggcgt
    23641 gccggttttt ggcccggaaa aagaagatga gttctggctg caaggcttgc ctgcgcaaag
    23701 tcgtatgttt ggtctggaag agtgccagcc gctgacgcca gatcgttggc tgaacgaagg
    23761 cgataccatc agcataggga atgtgacttt acaggtgtta cattgccccg ggcatacgcc
    23821 gggtcatgtg gtgtttttta atgagcaggc aaggctgctc atttctggcg atgttatttt
    23881 caaaggcgga gtagggcgca gtgatttccc gcgtggcgat cacaatcaac tgatttcttc
    23941 aatcaaagat aaattgctgc cactggggga tgacgtgaca tttattccgg gtcacggacc
    24001 attatccact ctcggttatg agcgcctgca taatcccttc ttgcaagacg aaatgcctgt
    24061 ctggtaaagg cgcataaaaa agcccgcttt caagcgggct tcatcggttt taacgcttac
    24121 agcactgcca caatcgcttc gcacagcggt gccatgttat ctggtgtcat cccggccacg
    24181 tttacgcgac cagaagcaac agcatatacg ccaaactctt cgcgcagacg cagcacttgt
    24241 tcttttgtca ggccactgaa ggagaacatg ccgttctgtt tgatgataaa gctgaagtcg
    24301 cgatttgcgc ctttttcctg cagcgtattg acgaacaact gacgcatacg ctgaatacgc
    24361 tggcgcatat cagtcagttc ttgttcccaa atcgcacgta acgcatcgtt gctcaggatg
    24421 gtggcaacaa cagaagcgcc gtgtgctggt gggttagagt agttagcacg aatcgccgct
    24481 ttcatttggc tgaatgcacg ttcaacagtt tcactgtcag cagcaaccag agtacaagcg
    24541 ccaacacgct cgttgtacag gccaaagttt ttagagtagg aactggcaac aatcagctcc
    24601 ttatgcagag ccgcgaaagc gcgcagtcct tcagcatctt cttccagacc ccgagcaaaa
    24661 ccctggtaag cgaagtcaaa cagcggtaac cagcctttct caacggagag ttgtgccagt
    24721 gtctgccatt gttccagcgt agggtcgatg ccggttgggt tatggcagca gccatggaac
    24781 agcactacgt cgccagcctg ggcttcgttc aggctgttaa tcagtgcatc gaagtcaaga
    24841 gtgtgatttt ccgcatcata ataagcgtat tcacgaactt ccagacctgc agagttaaag
    24901 acgctcttat ggttcggcca gcttgggttg ctcacccaca cacgcttaac gctggtattt
    24961 tttgccagga aatcggcagc cacgcgtagt gcgccagtgc cacccggagt ctgtgccgtg
    25021 cgagcacgtt tgtcattgat cagggcgcta cctttaccaa acagcagttc ctgagtgcag
    25081 cgaccaaatt cagggatgcc gtcaatgccg aggtaatttt tggtggtttc attttcgagc
    25141 agatactgtt cagccttttt cacgctggtc agtaccgggg ttttacccgt ctcatcttta
    25201 tagacaccaa tcccgaggtt aattttgccg ggacgttcat cggcacgaaa cagatcggcc
    25261 aggcccagaa tcgggtcggc aggagcggcg gtaatgttct caaacatgac gaggttccat
    25321 tatggttaca gaagggaagt ccgctatcag ggtaacggga gatttacaaa attccaacta
    25381 ttactgatga aaacgcaggc tgtttttgca agacgtgaga ttgctctgga aggcataaaa
    25441 aaaacaggac caaagtcctg ttttttcggc atttaacaaa gaggtgtgct attagaactg
    25501 gtaaacgata cccacagcaa cggtatcgtc ggaacctacg cccaacttgt tgtcagaatc
    25561 gatctggttg atgatgtagt caacataggt ggacatgttt ttgttgaagt agtaggttgc
    25621 gcccacttca aagtagttca ccagatcaac atcaccgata ccgtctacgt ctttcgcttt
    25681 agatttagtg taagcgatgg acggacgcag accgaaatcg aactggtatt gcgcaactaa
    25741 cagaacgtct tgcgttttgt tggcgaagcc gctggtgttt gtaaatttgt tagtgatcgg
    25801 cgtagcgtta cgggtttcac cgtagttcgc tgccaggtag atgttgttcg cgtcatactt
    25861 cagaccagta gcccactgtt cagctttttt gcctttgcca aggggagact cttcctgcag
    25921 gttggtacgg tcagcggcac cataagcacc aacgatacca aagccttcgt attcgtagct
    25981 gatagaaccg ccaacaccgt cgccgttaga gcggcgtgca gtgtcacgct cgtttttacc
    26041 caggtactga acagcaaagt tcagaccatc aaccagacca aagaagttgg agttacgata
    26101 ggtagcaacg ccgccaacac gaccaacgaa gaagtcatcg ctgtatgcag tatcaccacc
    26161 aaattctggc agcatatcgg tgtaacccag tgcatcataa accacaccgt agttacggcc
    26221 gtaatcgaaa gaaccaacgt cagcgtattt aagacccgcg aatgccagac gcgttttgtt
    26281 accagtttga gcgtcagcgc cttcagagtt gttaccctgg aagttatatt cccactgacc
    26341 ataaccggtc agatcggaat tgatttgagt ttccccttta aaaccaagac gggcataggt
    26401 catgtcgcca ttgccaccgt aactgtcttc accgttaccc ttggagaaat aatgcagacc
    26461 gacagcttta ccgtacagat ctactttgtt gccatctttg ttatagattt ctgcagcgtt
    26521 tgcagtacct gctactaaca gagcagggac gatcactgcc agaatattgc gcttcatcat
    26581 tatttattac cctcatggtt ttttttatga cacctgccac tgccgtcaat aagttctgtc
    26641 aataaaaatt tacggaacta ttgatgagag t
//
LOCUS       DAHHTG010000063        25968 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.063, whole genome shotgun sequence.
ACCESSION   DAHHTG010000063 DAHHTG010000000
VERSION     DAHHTG010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 25968)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25968)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25968
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.063"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            33..1238
                     /gene="yedE"
                     /locus_tag="NBI74_002535"
     CDS             33..1238
                     /gene="yedE"
                     /locus_tag="NBI74_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium metabolism membrane protein YedE/FdhT"
                     /protein_id="HCD1932854.1"
                     /translation="MSWQQFKHAWLIKFWAPIPAVIAAGILSTYYFGITGTFWAVTGE
                     FTRWGGQLLQLFGVHAEEWGYFKIIHLEGSPLTRIDGMMILGMFGGCFAAALWANNVK
                     LRMPRSRIRIMQAIIGGIIAGFGARLAMGCNLAAFFTGIPQFSLHAWFFAIATAIGSW
                     FGARFTLLPIFRIPVKMQKVSAASPLTQKPDQARRRFRLGMLVFFGMLGWALLTAMNQ
                     PKLGLAMLFGVGFGLLIERAQICFTSAFRDMWITGRTHMAKAIIIGMAVSAIGIFSYV
                     QLGVEPKIMWAGPNAVIGGLLFGFGIVLAGGCETGWMYRAVEGQVHYWWVGLGNVIGS
                     TILAYYWDDFAPALATDWDKINLLKTYGPMGGLLVTYLLLFAALMLIIGWEKRFFRRA
                     APQIAKEIA"
     gene            1235..1468
                     /gene="yedF"
                     /locus_tag="NBI74_002536"
     CDS             1235..1468
                     /gene="yedF"
                     /locus_tag="NBI74_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase-like selenium metabolism
                     protein YedF"
                     /protein_id="HCD1932855.1"
                     /translation="MKNIVPDYRLDMVGEPCPYPAVATLEAMLQLKKGEILEVVSDCP
                     QSINNIPLDARNHGYTVLDIQQDGPTIRYLIQK"
     gene            1577..2074
                     /locus_tag="NBI74_002537"
                     /pseudo
     CDS             1577..2074
                     /locus_tag="NBI74_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000334561.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase family
                     protein"
     gene            complement(1956..2285)
                     /locus_tag="NBI74_002538"
     CDS             complement(1956..2285)
                     /locus_tag="NBI74_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001324015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCD1932856.1"
                     /translation="MKRLQAFKFQLRPGGQQEREMRRFAGACRFVFNRALALQNENHE
                     AGNKYIPYGKMASWLVGWKNATETQWLKDSPSQPLQQSLKDLERAYKNFFRLRHRAQT
                     VCYLSRL"
     gene            2293..2832
                     /gene="tnpA"
                     /locus_tag="NBI74_002539"
     CDS             2293..2832
                     /gene="tnpA"
                     /locus_tag="NBI74_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001407245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HCD1932857.1"
                     /translation="MIFWSVKKETDIRRGRHCVFLKQVHLVFVTKYRRQIFDHDATEK
                     LRTYFSNVCADFEAERVEMDGGPDHVHLLINYPPKLAISSLVNSLKGVSSRLLRRDRP
                     DIAVRYYDKGVLWSPGYFVSSCILTYQLDLITTISVPNRISNVISIPLIIPSTSCNFP
                     TLELHIVQTPDAKKSHVHF"
     gene            complement(2989..3786)
                     /locus_tag="NBI74_002540"
     CDS             complement(2989..3786)
                     /locus_tag="NBI74_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCD1932858.1"
                     /translation="MLAKDKTNLKIEEIRMHKHHEIHRVKPLMPALCRIRQGKKVINW
                     ETHSLTVDNNQIILFPCGYEFYIANYPEAGLYLAEMLYYPIDLIEKFQKFYAITDQIR
                     NTTGFCLPQNPELIYCWEQLKTSISRGFSTQIQEHLAMGVLLSLGAHHVNCLLLSDSK
                     QSLISRCYNLMLSEPGTKWTANKVARYLYISVSTLHRRLASEGVSFQSILDDVRLNNA
                     LSAIQTTVKPISEIARENGYKCPSRFTERFHNRFKITPRELRKASRE"
     gene            complement(3796..4347)
                     /gene="ybcL"
                     /locus_tag="NBI74_002541"
     CDS             complement(3796..4347)
                     /gene="ybcL"
                     /locus_tag="NBI74_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Raf kinase inhibitor-like protein YbcL"
                     /protein_id="HCD1932859.1"
                     /translation="MKKLIVSSVLAFITFSAQAAAFQVTSNEIKTGEQLTTSHVFSGF
                     GCEGGNTSPSLTWSGAPEGTKSFAVTVYDPDAPTGSGWWHWTVANIPATVTYLPADAG
                     RRDGTKLPTGAVQGRNDFGYAGFGGACPPKGDKPHHYQFKVWALKTDKIPVDSNSSGA
                     LVGYMLNANKIATAEITPVYEIK"
     gene            complement(4516..4848)
                     /gene="emrE"
                     /locus_tag="NBI74_002542"
     CDS             complement(4516..4848)
                     /gene="emrE"
                     /locus_tag="NBI74_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMR family multidrug efflux protein EmrE"
                     /protein_id="HCD1932860.1"
                     /translation="MNPYIYLGGAILAEVIGTTLMKFSEGFTRLWPSVGTIICYCASF
                     WLLAQTLAYIPTGIAYAIWSGVGIVLISLLSWGFFGQRLDLPAIIGMMLICAGVLVIN
                     LLSRSAPH"
     gene            complement(5192..5506)
                     /gene="fliE"
                     /locus_tag="NBI74_002543"
     CDS             complement(5192..5506)
                     /gene="fliE"
                     /locus_tag="NBI74_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HCD1932861.1"
                     /translation="MSAIQGIEGVISQLQATAMSARAQESLPQPTISFAGQLHAALDR
                     ISDTQTAARTQAEKFTLGEPGVALNDVMTDMQKASVSMQMGIQVRNKLVAAYQEVMSM
                     QV"
     gene            5721..7379
                     /gene="fliF"
                     /locus_tag="NBI74_002544"
     CDS             5721..7379
                     /gene="fliF"
                     /locus_tag="NBI74_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar M-ring protein FliF"
                     /protein_id="HCD1932862.1"
                     /translation="MNATAAQTKSLEWLNRLRANPKIPLIVAGSAAVAVMVALILWAK
                     APDYRTLFSNLSDQDGGAIVSQLTQMNIPYRFSEASGAIEVPADKVHELRLRLAQQGL
                     PKGGAVGFELLDQEKFGISQFSEQVNYQRALEGELSRTIETIGPVKGARVHLAMPKPS
                     LFVREQKSPSASVTVNLLPGRALDEGQISAIVHLVSSAVAGLPPGNVTLVDQGGHLLT
                     QSNTSGRDLNDAQLKYASDVEGRIQRRIEAILSPIVGNGNIHAQVTAQLDFASKEQTE
                     EQYRPNGDESHAALRSRQLNESEQSGSGYPGGVPGALSNQPAPANNAPISTPPANQNN
                     RQQQASTTSNSGPRSTQRNETSNYEVDRTIRHTKMNVGDVQRLSVAVVVNYKTLPDGK
                     PLPLSNEQMKQIEALTREAMGFSEKRGDSLNVVNSPFNSSDESGGALPFWQQQAFIDQ
                     LLAAGRWLLVLLVAWLLWRKAVRPQLTRRAEAVKTVQQQALAREEVEDAVEVRLSKDE
                     QLQQRRANQRLGAEVMSQRIREMSDNDPRVVALVIRQWINNDHE"
     gene            7372..8367
                     /gene="fliG"
                     /locus_tag="NBI74_002545"
     CDS             7372..8367
                     /gene="fliG"
                     /locus_tag="NBI74_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HCD1932863.1"
                     /translation="MSNLTGTDKSVILLMTIGEDRAAEVFKHLSQREVQTLSAAMANV
                     TQISNKQLTDVLAEFEQEAEQFAALNINANDYLRSVLVKALGEERAASLLEDILETRD
                     TASGIETLNFMEPQSAADLIRDEHPQIIATILVHLKRAQAADILALFDERLRHDVMLR
                     IATFGGVQPAALAELTEVLNGLLDGQNLKRSKMGGVRTAAEIINLMKTQQEEAVITAV
                     REFDGELAQKIIDEMFLFENLVDVDDRSIQRLLQEVDSESLLIALKGAEQPLREKFLR
                     NMSQRAADILRDDLANRGPVRLSQVENEQKAILLIVRRLAETGEMVIGSGEDTYV"
     gene            8360..9046
                     /gene="fliH"
                     /locus_tag="NBI74_002546"
     CDS             8360..9046
                     /gene="fliH"
                     /locus_tag="NBI74_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707825.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliH"
                     /protein_id="HCD1932864.1"
                     /translation="MSDNLPWETWTPDDLAPPPAEFVPMVESEETIIEEAEPSLEQQL
                     AQLQMQAHEQGYQAGIAEGRQQGHEQGYQEGLAQGLEQGLAEAKAQQAPIHARMQQLV
                     SEFQTTLDALDSVIASRLMQMALEAARQVIGQTPTVDNSALIKQIQQLLQQEPLFSGK
                     PQLRVHPDDLQRVDDMLSATLSLHGWRLRGDPTLHPGGCKVSADEGDLDASVATRWQE
                     LCRLAAPGVV"
     gene            9046..10419
                     /gene="fliI"
                     /locus_tag="NBI74_002547"
     CDS             9046..10419
                     /gene="fliI"
                     /locus_tag="NBI74_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellum-specific ATP synthase FliI"
                     /protein_id="HCD1932865.1"
                     /translation="MTTRLTRWLTTLDNFEAKMAQLPAVRRYGRLTRATGLVLEATGL
                     QLPLGATCVIERQNGSETHEVESEVVGFNGQRLFLMPLEEVEGVLPGARVYAKNISAE
                     GLQSGKQLPLGPALLGRVLDGSGKPLDGLPSPDTTETGALITPPFNPLQRTPIEHVLD
                     TGVRPINALLTVGRGQRMGLFAGSGVGKSVLLGMMARYTRADVIVVGLIGERGREVKD
                     FIENILGAEGRARSVVIAAPADVSPLLRMQGAAYATRIAEDFRDRGQHVLLIMDSLTR
                     YAMAQREIALAIGEPPATKGYPPSVFAKLPALVERAGNGISGGGSITAFYTVLTEGDD
                     QQDPIADSARAILDGHIVLSRRLAEAGHYPAIDIEASISRAMTALISEQHYARVRTFK
                     QLLSSFQRNRDLVSVGAYAKGSDPMLDKAIALWPQLEGYLQQGIFERADWEASLQGLE
                     RIFPTVS"
     gene            10438..10881
                     /gene="fliJ"
                     /locus_tag="NBI74_002548"
     CDS             10438..10881
                     /gene="fliJ"
                     /locus_tag="NBI74_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis chaperone FliJ"
                     /protein_id="HCD1932866.1"
                     /translation="MAEHGALATLKDLAEKEVEDAARLLGEMRRGCQQAEEQLKMLID
                     YQNEYRNNLNSDMSAGMTSNRWINYQQFIQTLEKAITQHRQQLNQWTQKVDIALNSWR
                     EKKQRLQAWQTLQERQSTAALLAENRLDQKKMDEFAQRAAMRKPE"
     gene            10878..12005
                     /gene="fliK"
                     /locus_tag="NBI74_002549"
     CDS             10878..12005
                     /gene="fliK"
                     /locus_tag="NBI74_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook length control protein FliK"
                     /protein_id="HCD1932867.1"
                     /translation="MIRLAPLITADVDTTTLPGGKASDAAQDFLALLSEALAGETTTD
                     KAAPQLLVATDKPTTKGEPLVSDIVSDAQQADLLIPVDETLPVINDEQSTSTPLTTAQ
                     TMTLAAVADNNTAKDEKADDLNEDVTASLSALFAMLPGFDNTPKVTDAPSTVLPTEKP
                     TLFTKLTSAQLTTAQPDDAPGTPAQPLTPLVAEAQSKAEVISTPSPVTAAASPLITPH
                     QTQPLPTVAAPVLSAPLGSHEWQQSLSQHISLFTRQGQQSAELRLHPQDLGEVQISLK
                     VDDNQAQIQMVSPHQHVRAALEAALPVLRTQLAESGIQLGQSNISGESFSGQQQAASQ
                     QQQSQRTANHEPLTGEDDDTLPVPVSLQGRVTGNSGVDIFA"
     gene            12110..12574
                     /gene="fliL"
                     /locus_tag="NBI74_002550"
     CDS             12110..12574
                     /gene="fliL"
                     /locus_tag="NBI74_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated protein FliL"
                     /protein_id="HCD1932868.1"
                     /translation="MTDYAISKKSKRSLWIPILVFITLAACASAGYSYWHSHQVAADD
                     KAQQRVVPSPVFYALDTFTVNLGDADRVLYIGITLRLKDEATRSRLSEYLPEVRSRLL
                     LLFSRQDAAVLATEEGKKNLIAEIKTTLSTPLVAGQPKQDVTDVLYTAFILR"
     gene            12579..13583
                     /gene="fliM"
                     /locus_tag="NBI74_002551"
     CDS             12579..13583
                     /gene="fliM"
                     /locus_tag="NBI74_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HCD1932869.1"
                     /translation="MGDSILSQAEIDALLNGDSEVKDEPTASVSGESDIRPYDPNTQR
                     RVVRERLQALEIINERFARHFRMGLFNLLRRSPDITVGAIRIQPYHEFARNLPVPTNL
                     NLIHLKPLRGTGLVVFSPSLVFIAVDNLFGGDGRFPTKVEGREFTHTEQRVINRMLKL
                     ALEGYSDAWKAINPLEVEYVRSEMQVKFTNITTSPNDIVVNTPFHVEIGNLTGEFNIC
                     LPFSMIEPLRELLVNPPLENSRNEDQNWRDNLVRQVQHSQLELVANFADISLRLSQIL
                     KLKPGDVLPIEKPDRIIAHVDGVPVLTSQYGTLNGQYALRIEHLINPILNSLNEEQPK
                     "
     gene            13580..13993
                     /gene="fliN"
                     /locus_tag="NBI74_002552"
     CDS             13580..13993
                     /gene="fliN"
                     /locus_tag="NBI74_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HCD1932870.1"
                     /translation="MSDMNNPADDNNGAMDDMWAEALSEQKSTSGKSAADAMFQQFGG
                     GDVSGTLQDIDLIMDIPVKLTVELGRTRMTIKELLRLTQGSVVALDGLAGEPLDILIN
                     GYLIAQGEVVVVADKYGVRITDIITPSERMRRLSR"
     gene            13996..14361
                     /gene="fliO"
                     /locus_tag="NBI74_002553"
     CDS             13996..14361
                     /gene="fliO"
                     /locus_tag="NBI74_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FliO"
                     /protein_id="HCD1932871.1"
                     /translation="MNNHATVQSSAPVSAAPLLQVSGALIAIIALILAAAWLVKRLGF
                     APKRTGVNGLKISASASLGARERVVVVDVEDARLVLGVTAGQINLLHKLPPSAPTEEI
                     PQTDFQSVMKNLLKRGGRS"
     gene            14361..15098
                     /gene="fliP"
                     /locus_tag="NBI74_002554"
     CDS             14361..15098
                     /gene="fliP"
                     /locus_tag="NBI74_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310714.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HCD1932872.1"
                     /translation="MRRLFSVAPVLLWLITPLAFAQLPGITSQPLPGGGQSWSLPVQT
                     LVFITSLTFIPAILLMMTSFTRIIIVFGLLRNALGTPSAPPNQVLLGLALFLTFFIMS
                     PVIDKIYVDAYQPFSEEKISMQEALEKGAQPLREFMLRQTREADLGLFARLANTGPLQ
                     GPEAVPMRILLPAYVTSELKTAFQIGFTIFIPFLIIDLVIASVLMALGMMMVPPATIA
                     LPFKLMLFVLVDGWQLLVGSLAQSFYS"
     gene            15108..15377
                     /gene="fliQ"
                     /locus_tag="NBI74_002555"
     CDS             15108..15377
                     /gene="fliQ"
                     /locus_tag="NBI74_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013365797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HCD1932873.1"
                     /translation="MTPESVMMMGTEAMKVALALAAPLLLVALVTGLIISILQAATQI
                     NEMTLSFIPKIIAVFIAIIIAGPWMLNLLLDYVRTLFTNLPYIIG"
     gene            15386..16171
                     /gene="fliR"
                     /locus_tag="NBI74_002556"
     CDS             15386..16171
                     /gene="fliR"
                     /locus_tag="NBI74_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FliR"
                     /protein_id="HCD1932874.1"
                     /translation="MMQVTSDQWLSWLSLYFWPLLRVLALISTAPILSERSVPKRVKL
                     GLAMMITFAIAPSLPANDVPVFSFFALWLAVQQILIGIALGFTMQFAFAAVRTAGEII
                     GLQMGLSFATFVDPGSHLNMPVLARIMDMLALLLFLTFNGHLWLISLLVDTFHTLPIG
                     SEPLNSNAFLALTKAGSLIFLNGLMLALPLITLLLTLNLALGLLNRMAPQLSIFVIGF
                     PLTLTVGISLMAALMPLIAPFCEHLFSEIFNLLADIISELPLI"
     gene            16461..17084
                     /gene="rcsA"
                     /locus_tag="NBI74_002557"
     CDS             16461..17084
                     /gene="rcsA"
                     /locus_tag="NBI74_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RcsA"
                     /protein_id="HCD1932875.1"
                     /translation="MSTIIMDLCSYTRLGLTGYLLSRGVKKREINDIETVDDLAIACD
                     LQRPSVVFINEDCFIHDASNSQRIKHIINQHPNTLFIVFMAIANVHFDEYLLVRKNLL
                     ISSKSIKPESLDDILGDILKKETTITSFLNMPTLSLSRTESSMLRMWMAGQGTIQISD
                     QMNIKAKTVSSHKGNIKRKIKTHNKQVIYHVVRLTDNVTNGIFVNMR"
     gene            complement(17128..17316)
                     /gene="dsrB"
                     /locus_tag="NBI74_002558"
     CDS             complement(17128..17316)
                     /gene="dsrB"
                     /locus_tag="NBI74_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein DsrB"
                     /protein_id="HCD1932876.1"
                     /translation="MKVNDRVTVKTDGGPRRPGVVLAVEEFSEGTMYLVSLEDYPLGI
                     WFFNEAGHQDGIFVEKAE"
     gene            17479..17706
                     /gene="yodD"
                     /locus_tag="NBI74_002559"
     CDS             17479..17706
                     /gene="yodD"
                     /locus_tag="NBI74_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416463.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid resistance protein YodD"
                     /protein_id="HCD1932877.1"
                     /translation="MKTAKEYSDTAKREVSVDVDALLAAINEISESEVHRSQNDSEHV
                     SVDGREYHTWRELADAFELDIHDFSVSEVNR"
     gene            18004..18819
                     /locus_tag="NBI74_002560"
     CDS             18004..18819
                     /locus_tag="NBI74_002560"
                     /EC_number="3.1.3.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-3-phosphoglycerate phosphatase-related
                     protein"
                     /protein_id="HCD1932878.1"
                     /translation="MFSIQQPLLVFSDLDGTLLDSHSYDWQPAAPWLSRLHEANIPVI
                     LCSSKTSAEMLYLQKMLGLQGLPLIAENGAVIQLAEQWQDIDGFPRIISGISHGEICQ
                     VLNTLREKEHFKFTTFDDVDDATIAEWTGLSRSQAALTQLHEASVTLIWRDSDEHMAQ
                     FIARLNELGLQFMQGARFWHVLDASAGKDQAANWIIATYQQLSGRRPTTLGLGDGPND
                     APLLEVMDYAVIVKGLNREGVHLHDEDPARVWRTQREGPEGWREGLDHFFPAR"
     gene            complement(18816..20510)
                     /gene="yedQ"
                     /locus_tag="NBI74_002561"
     CDS             complement(18816..20510)
                     /gene="yedQ"
                     /locus_tag="NBI74_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis regulator YedQ"
                     /protein_id="HCD1932879.1"
                     /translation="MQHETKMENQSWLKKLARRLGPGHVVNLCFIVVLLFSTLLTWRE
                     VVVLEDAYISSQRNHLENVANALDKHLQYNVDKLIFLRNGMREALVAPLDFISLRNAV
                     TEFEQHRDEHAWQIELNRRRTLPVNGVSDALVSEGNLLSRENESLDNEITAALEVGYL
                     LRLAHNSSSMVEQAMYVSRAGFYVSTQPTLFTRNVPTRYYGYVTQPWFIGHSQRENRH
                     RAVRWFTSQPEHASNTEPQVTVSVPVDSNNYWYGVLGMSIPVRTMQQFLRNAIDKNLD
                     GEYQLYDSKLRFLTSSNPDHPTGNIFDPRELALLAQAMEHDTRGGIRMDSRYVSWERL
                     DHFDGVLVRVHTLSEGVRGDFGSISIALTLLWALFTSMLLLSWYVIRRMVSNMYVLQS
                     SLQWQAWHDTLTRLYNRGALFEKACPLAKLCQTHQHPFSVIQVDLDHFKAINDRFGHQ
                     VGDRVLSHAAGLISSSLRAQDVAGRVGGEEFCVILPGANLTQAAEVAERIRLKLNEKE
                     MLIAKSTTIRISASLGVSSSEETGDYDFEQLQSLADRRLYLAKQAGRNRVFASDNA"
     gene            complement(20681..20863)
                     /locus_tag="NBI74_002562"
     CDS             complement(20681..20863)
                     /locus_tag="NBI74_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2158 domain-containing protein"
                     /protein_id="HCD1932880.1"
                     /translation="MSFMVSEEVTVKEGGPRMIVTGYSSGMVECRWYDGYGVKREAFH
                     ETDLVPGEGSRSAEEV"
     gene            complement(20942..21859)
                     /locus_tag="NBI74_002563"
     CDS             complement(20942..21859)
                     /locus_tag="NBI74_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 domain-containing protein"
                     /protein_id="HCD1932881.1"
                     /translation="MLLAGSSLLTLLDDIATLLDDISVMGKLAAKKTAGVLGDDLSLN
                     AQQVSGVRANRELPVVWGVAKGSLINKVILVPLALIISAFIPWAITPLLMIGGAFLCF
                     EGVEKVLHMLEARKHKEDPAQSQQRLEKLAAQDPLKFEKDKIKGAIRTDFILSAEIVA
                     ITLGIVAEAPLLNQVLVLSGIALVVTVGVYGLVGVIVKIDDLGYWLAEKSSALMQALG
                     KGLLIIAPWLMKALSIVGTLAMFLVGGGIVVHGIAPLHHAIEHFAGQQSAVVAMILPT
                     VLNLILGFIIGGIVVLGVKAVAKMRGQAH"
     gene            22032..22952
                     /gene="yedA"
                     /locus_tag="NBI74_002564"
     CDS             22032..22952
                     /gene="yedA"
                     /locus_tag="NBI74_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="drug/metabolite exporter YedA"
                     /protein_id="HCD1932882.1"
                     /translation="MRFRQLLPLFGALFALYIIWGSTYFVIRIGVESWPPLMMAGVRF
                     LAAGILLLAFLLLRGHKLPPLRPLLNAALIGLLLLAVGNGMVTVAEHQNVPSGIAAVV
                     VATVPLFTLCFSRLFGIKTRKLEWVGIAIGLAGIIMLNSGGNLSGNPWGAILILIGSI
                     SWAFGSVYGSRITLPVGMMAGAIEMLAAGVVLMIASMIAGEKLTALPSLSGFLAVGYL
                     ALFGSIIAINAYMYLIRNVSPALATSYAYVNPVVAVLLGTGLGGETLSKIEWLALGVI
                     VFAVVLVTLGKYLFPAKPVVAPVIQDASSE"
     gene            complement(22941..23411)
                     /gene="vsr"
                     /locus_tag="NBI74_002565"
     CDS             complement(22941..23411)
                     /gene="vsr"
                     /locus_tag="NBI74_002565"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VSPR family DNA mismatch endonuclease"
                     /protein_id="HCD1932883.1"
                     /translation="MVDVHDKATRSKNMRAIATRDTAIEKRLASLLTGQGLAFRVQDA
                     SLPGRPDFVVDEYRCVIFTHGCFWHHHHCYLFKVPATRTEFWLEKIGKNVERDRRDIS
                     RLQELGWRVLIVWECALRGREKLTDAALTERLEEWICGEGASAQIDTQGIHLLA"
     gene            complement(23392..24810)
                     /gene="dcm"
                     /locus_tag="NBI74_002566"
     CDS             complement(23392..24810)
                     /gene="dcm"
                     /locus_tag="NBI74_002566"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-cytosine methyltransferase"
                     /protein_id="HCD1932884.1"
                     /translation="MQENISVTDSYSTGNAAQAMLEKLLQIYDVKTLVAQLNGVGENH
                     WSAAILKRALANDSAWHRLSEKEFAHLQTLLPKPPEHHPHYAFRFIDLFAGIGGIRRG
                     FESIGGQCVFTSEWNKHAVRTYKANHYCDPATHHFNEDIRDITLSHQEGVSDEAAAEH
                     IRQHIPEHDVLLAGFPCQPFSLAGVSKKNSLGRAHGFACDTQGTLFFDVVRIIDARRP
                     AMFVLENVKNLKSHDKGKTFRIIMQTLDELGYDVADAEDNGPDDPKIIDGKHFLPQHR
                     ERIVLVGFRRDLNLKADFTLRDISECFPAQRVTLAQLLDPMVEAKYILTPVLWKYLYR
                     YAKKHQARGNGFGYGMVYPNNPQSVTRTLSARYYKDGAEILIDRGWDMATGEKDFDDP
                     LNQQHRPRRLTPRECARLMGFEAPGEAKFRIPVSDTQAYRQFGNSVVVLVFAAVAKLL
                     EPKIKQAVALRQQEAQHGRRSR"
     gene            complement(24877..25572)
                     /locus_tag="NBI74_002567"
     CDS             complement(24877..25572)
                     /locus_tag="NBI74_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HCD1932885.1"
                     /translation="MELQHWQAQFENWLKNHHQHQDAAHDLCHFRRVWATAQKLAADD
                     DVDMLVILTACYFHDIVSLAKNHPQRQRSSILAAEETRRLLREEFAQFPAEKIEAVCH
                     AIAAHSFSAQIAPLTTEAKIVQDADRLEALGAIGLARVFAVSGALGLALFDGEDPFAQ
                     HRPLDDKRYALDHFQTKLLKLPQTMQTARGKQLAQHNAQFLVEFMAKLGAELAGENEG
                     IDHKVIDAFSPAG"
     gene            complement(25612..25914)
                     /locus_tag="NBI74_002568"
     CDS             complement(25612..25914)
                     /locus_tag="NBI74_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001718444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HCD1932886.1"
                     /translation="MEYGSTKMEERLSRSPGGKLALWAFYTWCGYFVWAMARYIWVMS
                     RIPDAPVSGFESDLGSTAGKWLGALVGFLFMALVGALLGGIAWYTRPRPARCRRYE"
BASE COUNT         6347 a         6740 c         6554 g         6327 t
ORIGIN      
        1 tttcaaacct tctgagttca gaggctacat tcatgtcatg gcagcaattc aaacacgcct
       61 ggttgattaa attctgggcg cccatccccg cggtcatcgc ggcgggtatt ctctccactt
      121 actattttgg catcactggc accttttggg cagtcacggg cgaatttacc cgttggggcg
      181 gtcagctcct gcagttgttc ggcgtccatg ctgaagagtg gggttatttt aaaattatcc
      241 atctggaagg atcgccatta acccgcatcg acggaatgat gatcctcggt atgtttggcg
      301 gctgttttgc cgcagcgctg tgggccaaca atgtcaaact gcgcatgccg cgcagccgta
      361 tccgcattat gcaggccatc attggcggta ttatcgccgg ttttggcgca cgtctggcaa
      421 tgggctgtaa cctggcggcg ttctttaccg gaattccaca gttctcgctg cacgcctggt
      481 tctttgccat cgccactgcc attggttcat ggtttggcgc gcgctttacc cttctgccca
      541 tcttccgtat tcccgtgaaa atgcagaaag tttctgctgc ctcaccgttg acgcaaaaac
      601 cggatcaggc gcggcgtcgt tttcgtctcg ggatgctggt ctttttcggc atgctgggct
      661 gggcgctgct gacggcgatg aaccagccca aactggggct ggcaatgctg tttggcgtcg
      721 gctttggttt actgattgaa cgtgcgcaaa tctgctttac ttcggcgttc cgcgatatgt
      781 ggatcaccgg acgtacccat atggcgaaag caatcattat cggtatggcg gtaagtgcca
      841 tcgggatctt cagttacgta cagttaggcg ttgaacccaa aatcatgtgg gcgggaccaa
      901 acgcggtaat tggtggttta ctgtttggtt ttggcatcgt gctggcaggc ggctgcgaaa
      961 ccggctggat gtaccgcgcg gtagaaggcc aggtgcacta ctggtgggtc ggtctgggca
     1021 acgtgatcgg atcaacgatt ctggcgtatt attgggatga tttcgctccg gcgctggcca
     1081 ccgactggga caaaatcaac ctgctgaaaa cctatggccc gatgggcggc ctgctggtga
     1141 catatttgct gttgtttgca gctctaatgt tgattatcgg ctgggaaaaa cgcttcttcc
     1201 gccgtgcggc accgcagatt gctaaggaga tcgcatgaaa aatatcgttc ctgattaccg
     1261 tcttgatatg gtgggtgaac cctgtcctta tccggcagtc gcaacccttg aggcgatgct
     1321 gcagttaaaa aaaggggaga tcctggaagt ggtgagcgac tgtccgcagt cgatcaataa
     1381 tattccactg gatgcgcgta atcacggcta tacggtgctg gatattcagc aagacgggcc
     1441 gaccattcgt tatttaattc agaagtaatc cctcattccc gctgtggccc acagcgggaa
     1501 catcgttacc tctctatcac atagatttta cccgccctct ctcctattct tttcagtgtg
     1561 acctcaggag gtttctatgt gtggacgctt tgcctaatcc caaacgcgtg aagattacct
     1621 tgcgcttctc gcggaagata ttgaacgcga tattccctac gatcccgaac ctattggcag
     1681 atacaacgtc gcgccgggaa ccaaagtcct tctgctcagt gaacgtgatg aacaccttca
     1741 tctggatccg gttttctggg gatatgctcc cggatggtgg gataaaccgc cgctggttaa
     1801 cgcccgcgta gaaactgccg caaccagtcg catgtttaaa ccgctctggc aacatggtcg
     1861 ggcaatctgc tttgccgatg gttggtttga gtggaaaaaa gaaggcgaca aaaaacagcc
     1921 ttattttatc tatcgcgctg atggacaacc tgtttttata gccgcgatag gtagcacacc
     1981 gtttgagcgc ggtgacgaag ccggaagaag tttttgtaag cccgctcaag gtctttcagt
     2041 gactgttgca atggctgtga gggcgaatct ttaagccatt gcgtttcagt ggcatttttc
     2101 cacccaacca accaggaagc cattttcccg taagggatgt atttattacc ggcctcatga
     2161 ttctcattct gaagcgccag agcacgattg aaaacgaaac gacatgcgcc tgcgaagcgc
     2221 ctcatttcac gctcctgttg accaccgggt cttaactgga atttaaatgc ctgtagtcgt
     2281 ttcacaggtc aaattatatt ttggtctgtg aaaaaagaaa ccgatatccg acgcggaagg
     2341 cattgtgttt tcctgaagca ggttcacctg gtctttgtca ctaaataccg acgtcagatt
     2401 tttgaccatg acgcgacaga aaaactacgc acttactttt caaatgtatg tgctgatttt
     2461 gaagctgaac gggttgaaat ggatggcgga ccagatcacg tccatttgtt aattaactat
     2521 cctcccaaac tggcgatatc cagtctggta aacagcctca aaggcgtatc gagtaggtta
     2581 ctgcgacgag atcgaccaga tattgcagtc aggtattacg acaaaggcgt tttgtggagt
     2641 cctggctatt ttgtcagtag ctgcatctta acgtatcaac tagatttaat aacaacgata
     2701 tccgtaccta accggatatc taatgtcata agcatccctt taattatccc ctcaacctct
     2761 tgtaactttc ccactcttga gctccacata gtccaaacgc cggatgcaaa aaaatcacac
     2821 gtgcatttct gaaattgtga tggaggaaga tagtatcaat tatatgtgct tagaaacgtc
     2881 cccgattagc gcagaagtca ccggattgtt cagactccaa taggttgagt atgccgtatt
     2941 tattttccaa aataaaaatc gatgctgttg ctccttcata aaatactttt actctctgga
     3001 cgcttttctt agctctcttg gtgttatctt aaaacgatta tggaaccttt cagtaaaacg
     3061 agaaggacac ttgtaaccat tttccctggc aatctcgctg atgggtttta ctgtcgtttg
     3121 tatagcagac aacgcattat ttaacctcac gtcgtccaat atactttgaa aacttactcc
     3181 ctcgcttgcc agacggcgat gcaatgtgga aacagaaatg tagagatatc tcgctacctt
     3241 gtttgctgtc cattttgttc caggttcgga tagcattagg ttataacaac gacttattaa
     3301 tgattgttta ctatctgata aaagtaaaca attaacatga tgtgctccta atgaaagtag
     3361 aacgcccatt gctaagtgtt cctgaatttg cgttgagaag cctcgggaaa tagatgtttt
     3421 tagttgctcc caacaatata ttaactcggg gttctgaggt aagcagaaac ctgtcgtgtt
     3481 acgaatttga tcagttatcg cataaaattt ttgaaacttt tcaattagat caatgggata
     3541 ataaagcatt tctgcaagat aaagacctgc ttctggataa ttcgcaatat aaaattcata
     3601 tccacaggga aataatatta tctgattatt atcaacagtt agactatgcg tctcccaatt
     3661 gataactttt tttccctgac gaatacgaca taaagctggc ataagaggct taaccctgtg
     3721 aatctcatga tgtttatgca tccgtatttc ttcaatcttt aggttagtct tatcttttgc
     3781 cagcatactc ttaccctact ttatctcata aactggtgtt atctcagcgg ttgcaatttt
     3841 attggcatta agcatataac caactaacgc tccgctggaa ttagaatcca caggaatctt
     3901 atcagttttt agagcccata ctttaaactg gtaatgatgg ggtttatctc ctttgggagg
     3961 acatgctcca ccaaatcctg cataaccaaa atcatttcgg ccttgaacag caccagtcgg
     4021 aagttttgta ccatcacgtc ttcctgcatc agcgggcaaa tatgttacag ttgctggaat
     4081 attagcaaca gtccagtgcc accatccact gcctgtagga gcatctggat catacacagt
     4141 tacggcaaag cttttggtgc cttcaggagc accagaccag gttaatgagg gcgatgtatt
     4201 accaccttca cacccaaatc cagaaaatac atgagacgtt gtaagttgct ctcctgtttt
     4261 tatttcatta ctagtgacct gaaatgctgc ggcctgcgca gaaaatgtta tgaatgccaa
     4321 tacacttgaa acgataagtt ttttcataaa aacctcattg ttgtgaccta tcgttatttt
     4381 atttgatgtg ccttgatctc attatgcata aaggcgcaat gatcgcgcaa atgcaatcat
     4441 aattataaag gtgccccgaa cattagccat aatatgcaca aatcagattc ctgttatctc
     4501 aaggataatt attttttaat gcggtgcact tcgtgacaac aagttaatga ctaacacacc
     4561 ggcacaaatc aacatcatgc ctatgatagc tggcaggtcc agtcgttgac caaaaaatcc
     4621 ccaggaaagc aaactaatca gaacgatacc gactcctgac cagatagcat aagcaatccc
     4681 tgtaggtata taagccagcg tctgagctaa taaccagaat gatgcacaat agcaaataat
     4741 tgtgccaaca gagggccata accgtgtaaa cccctctgaa aacttcatta aggttgtacc
     4801 aatgacctct gcaagtattg cgccaccaag ataaatataa gggttcatag catattcttt
     4861 cctgttcata ctggagagaa ttgtactaca gtttgaacac aactcaccgt tttcatcctg
     4921 gtgtatgtgt atccaggggg ctctggcgtg gcaagagata aaggcaggaa caataatgat
     4981 cagaaggaac cccaggctaa cgaatgagtt cactcttcct gggagcaaag cgaagacaaa
     5041 acatgttatc gcgtttacgg atcgtggatg gcagaaaact ttcaggatca ggtatccatt
     5101 ttaaaccaga aattgagtga gtttgcccca tccatgcccc acgctgtggg atcggatata
     5161 ataaacaagc atagtcaaca ggttacaaaa cctacacctg catggacatc acttcctgat
     5221 acgccgccac cagcttatta cgcacctgaa tccccatttg catagaaact gaggcttttt
     5281 gcatatcggt catcacatcg tttagcgcca cgccgggctc accgagggtg aatttttctg
     5341 cctgcgtgcg ggcggctgtt tgtgtatcgc ttatgcgatc gagcgcggcg tgcagctgcc
     5401 cggcaaaact aattgtcggt tgcggcagtg attcctgcgc acgcgcactc atcgccgtag
     5461 cctgtaactg gctgataacc ccttcaatcc cctgtatcgc tgacattttc gtctcccgga
     5521 taatttctga tagcaaagcc taccagtaag tcaataagac aaaggcgcta aatagcaaca
     5581 aaaaaacggg tttattggcg gatagaaaaa aacgaaagca caaataatgg gagcgtcaat
     5641 ttttcgagtt tgctgacccg ggagtgagtc ttgttccact ttgccaataa cgccgtccat
     5701 aatcagccac gaggtgcgcg atgaatgcga ctgcagccca gacaaaatct cttgagtggc
     5761 ttaatcgcct gcgtgcgaat ccgaaaattc cattgattgt tgccggttcc gcagcagtgg
     5821 cggtcatggt cgcactgatc ctgtgggcga aagcccccga ctaccgcaca ttattcagca
     5881 atctttccga tcaggatggt ggcgcaattg tcagccaact gacgcaaatg aatattcctt
     5941 accgcttcag cgaagccagc ggcgctattg aggttccggc agataaagtt cacgaactgc
     6001 gcctgcgcct ggcgcaacaa ggtttgccca aaggcggcgc ggtcggtttc gaactgctcg
     6061 atcaggaaaa gtttggtatc agtcagttca gcgaacaggt gaattatcag cgggcgctgg
     6121 aaggcgagct ttctcgtacc atcgaaacta tcggcccggt aaaaggggcg cgcgtacatc
     6181 tggcaatgcc gaaaccgtct ttattcgtcc gtgaacaaaa atccccttct gcatcggtga
     6241 cggtaaatct gttacccggc cgcgcactcg atgaagggca aattagcgct attgtacatc
     6301 tggtttccag cgcggttgct ggtctgccgc caggaaacgt cacgctggtg gatcagggcg
     6361 ggcatctgtt aacccagtcc aataccagcg ggcgcgatct taatgacgct cagttgaaat
     6421 atgccagcga tgtcgaaggc cgtattcagc ggcgtattga agcgatcctg tcgcctattg
     6481 ttggtaacgg taatattcac gcccaggtca cggcgcaact ggacttcgcc agtaaagaac
     6541 aaacggaaga acagtatcgc cctaacggtg atgaatctca tgcggcgctt cgttcacgcc
     6601 agcttaatga gagcgagcaa agcggttccg gttatccggg cggcgtaccg ggggcgttgt
     6661 cgaatcaacc ggcacctgcg aataacgcgc caatcagcac gcctccggca aatcaaaata
     6721 accgccagca gcaggcgagc accaccagca atagtgggcc gcgtagcaca cagcggaatg
     6781 aaaccagtaa ctacgaagtc gatcgcacca ttcgtcatac caaaatgaac gtgggcgatg
     6841 tgcaacgtct gtcagtcgcg gtagtggtga attacaaaac cttgccagac ggtaaaccat
     6901 tgcctctcag caacgaacag atgaagcaaa ttgaagctct gacccgcgag gcgatgggct
     6961 tttctgaaaa acgcggtgat tcgctcaatg ttgttaactc gccgttcaat agcagtgacg
     7021 aaagcggcgg agcactgccg ttctggcaac agcaagcgtt tatcgatcag ttgctggctg
     7081 ccggtcgctg gttgctggta ctgctggtgg cgtggttgct gtggcggaaa gcggtacgcc
     7141 cgcagctaac acgtcgcgcc gaggcggtga agactgtgca gcaacaggcg ctggcccgcg
     7201 aggaagtgga agatgcggtg gaagtccgtc tgagcaaaga cgaacaactc caacaacggc
     7261 gcgctaacca acgactgggg gcagaagtca tgagccagcg tatccgtgaa atgtctgata
     7321 acgatccgcg cgtggtggcg ctggtcattc gccagtggat aaataacgat catgagtaac
     7381 ctgacaggca ccgataaaag cgtcatcctg ctgatgacca ttggcgaaga ccgggcggca
     7441 gaggtgttca agcacctctc ccagcgcgaa gtgcaaaccc tgagcgctgc aatggcgaac
     7501 gtcacgcaga tctccaacaa gcagctaacc gatgtgctgg cggagtttga gcaagaagct
     7561 gaacagtttg ccgcactgaa tatcaacgcc aacgattatc tgcgttcggt attggtcaaa
     7621 gctctgggtg aagaacgtgc cgccagcctg ctggaagata ttctcgaaac ccgcgatacc
     7681 gccagcggta ttgaaacgct caactttatg gagccgcaga gcgccgccga tctgattcgc
     7741 gatgagcatc cgcaaattat cgccaccatt ctggtgcatc tgaagcgcgc ccaggccgcc
     7801 gatattctgg cgttgttcga tgaacgcctg cgccacgacg tgatgttgcg tatcgccacc
     7861 tttggcggcg tgcagccagc cgcgctggcg gaactgaccg aagtactgaa tggcttgctc
     7921 gacggtcaga atctcaagcg cagcaaaatg ggcggcgtga gaacggcagc cgaaattatc
     7981 aacctgatga aaacgcagca ggaagaagcc gttattaccg ccgtgcgtga attcgacggc
     8041 gagctggcgc agaaaatcat cgacgagatg ttcctgttcg agaatctggt ggatgtcgat
     8101 gatcgcagca ttcagcgtct gttgcaggaa gtggattccg aatcgctgtt gatcgcgctg
     8161 aaaggggccg agcagccact gcgcgagaaa ttcctgcgca atatgtcgca gcgtgccgcc
     8221 gatattctgc gcgacgatct cgccaaccgt ggtccggtgc gtctgtcgca ggtggaaaac
     8281 gaacagaaag cgattctgct gattgtgcgc cgtcttgccg aaactggcga gatggtgatt
     8341 ggcagcggcg aggataccta tgtctgataa tctgccgtgg gaaacctgga cgccggacga
     8401 tctcgcgcca ccaccggcag agtttgtgcc aatggtcgag tcggaagaaa ccattattga
     8461 agaggccgaa cccagccttg agcagcaact ggcgcaactg caaatgcagg cccacgagca
     8521 gggttatcag gcagggattg ccgaaggtcg ccagcaaggt catgaacagg gctatcagga
     8581 aggactggcc caggggctgg aacaaggtct ggcagaggcg aaggctcaac aagcgccaat
     8641 tcatgcccgg atgcagcaac tggtcagcga atttcaaact acccttgatg cgcttgatag
     8701 tgtgattgct tcgcgcctga tgcagatggc gctggaggcg gcacgtcagg tgatcggtca
     8761 gacgccaacg gtggataact cggcactgat caaacagatc caacagttgt tgcagcaaga
     8821 accgttattc agcggtaaac cacagctgcg cgtacacccg gatgatctgc aacgtgttga
     8881 tgatatgctc agcgcaactt taagtttgca tggctggcgc ttgcggggcg atcccaccct
     8941 ccatcctggc ggttgcaaag tctccgccga tgaaggcgat ctcgacgcca gtgtcgccac
     9001 tcgctggcaa gaactctgcc gtctggcagc accaggagtg gtgtaatgac cacgcgcctg
     9061 actcgctggc taaccacgct ggataacttt gaagccaaaa tggcgcagtt gcctgcggta
     9121 cgtcgctacg ggcgattaac ccgcgctacc gggctggtgc tggaagccac cggattacaa
     9181 ttgccgctcg gcgcaacctg tgtcattgag cgccagaacg gcagcgaaac gcacgaagta
     9241 gaaagcgaag tcgtcggctt taacggtcaa cggctgtttt taatgccgct ggaggaagtg
     9301 gaaggtgtcc tgcccggcgc gcgtgtatat gccaaaaaca tttcggcaga agggctgcaa
     9361 agcggcaagc agttgccgct cggtccggcg ttattaggtc gtgttctgga cggcagcggt
     9421 aaaccgctcg atggcctccc ctcccccgat acgacggaaa ccggtgcgct gattaccccg
     9481 ccatttaacc cattgcaacg tacaccgatt gaacatgtgc tggacaccgg cgtgcgccca
     9541 atcaatgccc tgcttaccgt cgggcgcggg cagcgtatgg ggctgtttgc cgggtccggc
     9601 gttggtaaaa gtgtgctgct ggggatgatg gcccgttaca cccgcgccga tgtcattgtc
     9661 gtgggtttga ttggtgaacg agggcgcgaa gtaaaagatt ttattgagaa tattctcggt
     9721 gccgaaggac gtgcacgctc cgtggtgatt gccgctccgg cggatgtttc tccgctcctg
     9781 cgaatgcaag gtgccgccta tgccacgcgc attgccgaag attttcgcga tcgtggtcag
     9841 catgtgttgc tgattatgga ctccctcacc cgctacgcga tggcccagcg tgaaattgcg
     9901 ctggcgattg gcgaaccacc tgctactaaa ggttatccac cgtcggtgtt tgccaaatta
     9961 ccggcactgg tcgagcgtgc cggtaatggc attagcggcg gcggctcgat taccgcgttt
    10021 tataccgtgc tcaccgaagg cgatgaccag caggacccca ttgctgactc cgcgcgggca
    10081 atccttgacg gtcacattgt gctgtctcgc cgactggcgg aagccgggca ctatccggct
    10141 atcgatattg aagcgtcgat cagccgcgca atgacggcgt tgatcagtga gcaacattac
    10201 gcgcgagtgc gcaccttcaa acagctgttg tcgagttttc agcgtaaccg cgatctggtt
    10261 agcgtcggcg cgtatgccaa aggcagcgat ccgatgctcg acaaagccat cgccctgtgg
    10321 ccgcagctgg agggctattt gcaacaaggc atttttgaac gcgcggactg ggaagcgtct
    10381 ctccaggggc tggagcgtat tttcccgaca gtgtcataac ccaggagata acggcagatg
    10441 gcggaacatg gtgcgctggc gaccctgaaa gatctggcag aaaaagaggt agaggatgcc
    10501 gctcgcctgc tgggtgaaat gcgtcgcgga tgtcagcagg cggaagaaca gctcaaaatg
    10561 ctgatcgatt atcagaatga atatcgcaat aacctcaata gcgatatgag tgccgggatg
    10621 accagcaacc gctggatcaa ctatcagcag tttatccaga cgctggaaaa agccattact
    10681 cagcatcgcc agcaacttaa tcagtggacg cagaaagttg acattgccct gaacagctgg
    10741 cgagaaaaga aacaacgttt gcaggcctgg cagacactgc aggaacggca atccactgcg
    10801 gcactgcttg cagaaaaccg cctcgatcag aaaaagatgg atgagttcgc ccagcgcgcc
    10861 gccatgagga aacctgaatg attcgcttag cgcccttgat taccgccgac gttgacacca
    10921 ccacattgcc tggcggcaaa gccagcgatg ctgcacaaga ttttctcgcg ttgttgagcg
    10981 aagcattagc aggcgagaca acaaccgaca aagcggcccc ccagttgctg gtggcaacag
    11041 ataagcccac gacaaaaggc gaaccgctgg tcagcgatat tgtttccgat gcgcaacaag
    11101 cggatttact gatccctgtg gatgaaacac ttcctgtcat caacgacgaa caatccacat
    11161 caacaccgtt aaccaccgct cagacgatga cgttggctgc ggtggctgac aacaatacgg
    11221 caaaagacga aaaagcggat gatcttaatg aagacgtcac cgcaagtctg agcgcccttt
    11281 ttgcgatgtt gccgggtttt gacaatacgc ccaaagtgac tgatgcaccg tcaaccgtgt
    11341 taccgacaga gaaaccaacg cttttcacaa aactgacttc tgcgcaactc acaacagcac
    11401 agcctgatga cgcccccggc acaccagctc agccattaac accgctggta gcagaagccc
    11461 agagtaaagc ggaagtcatc agcacacctt cgccggtgac tgctgccgcc agcccgctaa
    11521 tcactccaca ccagacacag ccactgccca ccgtcgccgc gcctgttttg agtgcaccgc
    11581 tgggttctca cgaatggcaa caatcattaa gccagcatat ttcgctgttt acccgccagg
    11641 ggcaacaaag tgcagagttg cgtctgcacc cgcaggattt aggtgaagtg caaatttccc
    11701 tcaaagtgga tgataaccag gcgcaaatcc agatggtttc accgcatcag catgtacgcg
    11761 ccgccctgga agcagcgctg ccggtactgc gtacgcagct ggccgaaagt ggcattcagt
    11821 tagggcaaag caacatcagt ggcgaaagct ttagtggtca gcagcaggcc gcttcccagc
    11881 aacagcaaag ccaacgcaca gcaaaccatg aacctctgac gggggaagac gacgatacgc
    11941 ttccggttcc cgtctcttta caagggcgtg taacaggcaa cagcggcgtt gatattttcg
    12001 cctaacgtca gaggtagcac cgtaatccgc gtcttttccc cgctttgttg cgctcaagac
    12061 gcaggataat tagccgataa gcagtagcga cacaggaaga ccgcaacaca tgactgatta
    12121 cgcgataagc aagaaaagca agcgatcgct ttggatcccg attctggtat tcattaccct
    12181 cgcggcctgt gccagcgcag gttacagcta ctggcattcg catcaggttg ccgctgacga
    12241 caaagcgcag caacgcgtcg tgccctcccc ggtcttctat gcgctggata cctttacggt
    12301 caatttgggc gatgcggatc gcgtgcttta tatcggcata accctgcgac tgaaagatga
    12361 agcaacccgc tcgcggctga gtgagtattt gccggaagtc cgtagtcgct tgctgttact
    12421 gttttcacgt caggatgctg ccgtactggc gacagaagaa ggcaagaaaa acctgattgc
    12481 cgagattaaa accacacttt ccaccccgct tgttgccggg caaccgaaac aggatgtcac
    12541 cgacgtgctg tataccgctt ttattctgcg ataacgacat gggcgatagt attctttctc
    12601 aagctgaaat tgatgcgctg ttgaatggcg acagcgaagt caaagacgaa ccgacagcca
    12661 gtgttagcgg cgaaagtgac attcgtccgt acgatccgaa tacccaacga cgggttgtgc
    12721 gcgaacgttt gcaggcgctg gaaatcatta atgagcgctt tgcccgccat tttcgtatgg
    12781 ggctgttcaa cctgctgcgc cgtagcccgg atattaccgt cggggccatc cgcattcagc
    12841 cgtaccatga atttgcccgc aacctgccgg tgccgaccaa cctgaacctt attcatctga
    12901 aaccgctgcg cggcacaggg ctggtggtgt tctcgccgag tctggtgttt atcgccgtgg
    12961 ataacctgtt tggcggcgat ggacgcttcc cgaccaaagt ggaaggtcgc gagtttactc
    13021 ataccgaaca gcgcgtcatc aaccgcatgt taaagctggc gcttgaaggc tatagcgacg
    13081 cctggaaagc gattaatccg ctggaagttg agtacgtgcg ttcggaaatg caggtgaaat
    13141 ttaccaatat caccacctcg ccgaacgaca ttgtggttaa cacgccgttc catgtggaga
    13201 ttggcaacct gaccggcgaa tttaatatct gcctgccatt cagcatgatc gagccgctac
    13261 gggaattgtt ggttaacccg ccgctggaaa actcgcgtaa tgaagatcag aactggcgcg
    13321 ataacctggt gcgccaggtg cagcattcac agctggaact ggtggccaac tttgccgata
    13381 tctcgctacg cctgtcgcag attttaaaac tgaaacccgg cgacgtcctg ccgatagaaa
    13441 aacccgatcg catcatcgcc catgttgacg gcgtcccggt tctgaccagt cagtacggca
    13501 ctctcaacgg tcagtatgcg ttacggatag aacatttgat taacccgatt ttaaattctc
    13561 tgaacgagga acagcccaaa tgagtgacat gaataatccg gccgatgaca acaacggcgc
    13621 aatggacgat atgtgggctg aagcgttgag cgagcaaaaa tcaaccagcg gaaaaagcgc
    13681 tgccgacgca atgttccagc aatttggcgg cggagatgtc agcggaacgt tgcaggatat
    13741 cgacctgatt atggatattc cggtcaagct gaccgtcgag ctgggccgta cgcggatgac
    13801 catcaaagag ctgttgcgtc tgacgcaagg atcagtcgtg gcgctggacg gtctggcggg
    13861 cgaaccgctg gatattctga tcaacggtta tttaatcgcc cagggcgaag tggtggttgt
    13921 tgccgataaa tatggcgtgc ggatcaccga tatcattacc ccgtccgagc ggatgcgccg
    13981 cctgagccgt tagtgatgaa taaccatgct actgtgcaat cttccgcgcc ggtttctgct
    14041 gcgccactgc tgcaggtgag cggcgcactc atcgccatta ttgccctgat cctcgctgcc
    14101 gcctggctgg taaaacggct gggatttgcc cctaaacgca ctggcgttaa cggtctgaaa
    14161 attagcgcca gtgcttcgct gggcgcgcgt gaacgggtcg tggtggtcga tgtggaagat
    14221 gcacggctgg tgctcggcgt taccgcaggg caaatcaatc tgctacataa acttccccct
    14281 tcagcaccaa cggaagagat accgcagacc gattttcagt cggtcatgaa aaatttgctt
    14341 aagcgtggcg ggagatcctg atgcgtcgtt tgttttctgt cgcaccggtc cttctctggc
    14401 tgattacgcc cctcgccttc gcgcaactgc cgggtatcac cagccagccg ctacctggtg
    14461 gtggacaaag ctggtcgctc ccggtgcaga cgctggtgtt catcacctcg ttgacgttta
    14521 ttccggcaat tttactgatg atgaccagtt tcacccgcat cattattgtt tttggtttat
    14581 tgcgtaacgc gctgggaacg ccctccgcgc cacctaacca ggtattgctg gggctggcac
    14641 tgtttttgac cttttttatt atgtcgccag tgatcgacaa aatttatgtc gatgcgtacc
    14701 agccattcag cgaagagaaa atctcaatgc aggaggcgct ggaaaaaggg gcgcagccgc
    14761 tgcgtgagtt tatgctgcgt cagacccgcg aggcggattt agggttgttt gccagactgg
    14821 cgaataccgg cccgttgcag ggacctgaag ctgtgccgat gcgcattttg ctcccggcct
    14881 acgtgaccag cgagttgaaa accgcatttc agataggctt cacgattttc atcccttttt
    14941 tgattatcga cctggtgatt gccagcgtgt tgatggcatt ggggatgatg atggttcccc
    15001 ctgccaccat tgctctgccc tttaaactga tgctgtttgt actggtggat ggctggcaat
    15061 tgctggtcgg ttcgctggcg cagagctttt acagctagag aggcaaaatg acacctgaat
    15121 cggtcatgat gatgggaact gaagcgatga aagtcgcgct ggcactggca gccccgctgt
    15181 tgctagtggc gctggtcacg ggccttatca tcagtatttt gcaggcagcc acgcagatta
    15241 acgaaatgac gctgtcgttt attccgaaaa tcatcgccgt atttatcgcc attattattg
    15301 ccggaccgtg gatgctcaat ctgttgctgg attacgtccg caccttgttc actaacctgc
    15361 cgtatatcat cgggtagccc gaacaatgat gcaggtgaca agcgatcagt ggctttcctg
    15421 gttaagcctg tacttctggc cgttactgcg cgtgctggcg ctcatttcta ccgcgccaat
    15481 tctgagcgaa cgcagcgtgc cgaaacgggt aaaactgggt ctggcaatga tgatcacgtt
    15541 cgccattgcc ccatcattac ctgccaacga tgttcctgtt ttttcgttct ttgctctgtg
    15601 gctggccgtg cagcagatcc tgatcggcat tgcgcttggt tttaccatgc aatttgcctt
    15661 tgccgctgtg cgaaccgctg gcgaaattat cggtctgcaa atggggctgt catttgcgac
    15721 atttgtcgat cctggcagcc atctcaatat gcccgtttta gcgcgcatca tggatatgct
    15781 ggcattactg ctgttcctga catttaacgg tcatttatgg ttgatttcac tgctggtcga
    15841 tacctttcac accctgccga ttggtagcga accgttgaac agcaatgcgt ttctggcact
    15901 caccaaagca gggagtttga ttttccttaa cgggctgatg ctggcgttac cgctcattac
    15961 tctgttgctg accctgaatc tggcattagg tttactgaat cgtatggccc cgcaattatc
    16021 catttttgtt attggatttc cattaactct gactgtcggc atctctttaa tggcggcatt
    16081 aatgccgtta attgcgcctt tttgcgaaca tttattcagt gaaattttta atttgctggc
    16141 tgatattatt agtgaattac cattaatata attccgtaac gtttatcatg ctaccctaag
    16201 gattatccga aaaataatat ctgcgaacat cttccaggat actcctgtag cgaaatattt
    16261 gttttaagct ccctcacata tcgcaacatt tactttactt taagacaatt ccaggcaaat
    16321 tatacaacac tttacgggat agtaagtccg cctgaaaaat tgcgagagtg gcgcattagg
    16381 tgacccatgt tgttccgttt agtcatgatg aaatattcag gtaaggggaa ttatcgttac
    16441 gcattgagtg agggtatgcc atgtcaacga ttattatgga tttatgtagt tacacccgac
    16501 taggtttaac cgggtatctg ttgagtagag gggttaaaaa aagagaaatc aacgacattg
    16561 aaaccgttga tgaccttgcc atagcttgtg atttacagcg cccttcagtg gtgtttatta
    16621 atgaggactg tttcatccac gatgcttcta acagtcagcg catcaagcac atcattaatc
    16681 aacatcccaa tacgttattt atcgttttta tggcaattgc caatgttcat tttgatgagt
    16741 atctattggt cagaaaaaat ttattgatca gttctaaatc gattaaaccg gaatctctcg
    16801 acgatatcct tggcgatatt ctgaaaaaag agacaacgat aacctcgttt ttaaatatgc
    16861 cgacgttatc attgagccga accgaatcga gtatgttgcg aatgtggatg gcaggtcagg
    16921 gaaccattca aatctccgac caaatgaata tcaaagccaa gaccgtttca tcgcataaag
    16981 gtaatattaa acgtaagatc aaaacgcata ataaacaggt tatctaccat gtcgtccgac
    17041 tgacggataa tgtgactaat ggtatttttg tcaacatgcg ctaacacatt ctgactggtg
    17101 gtttcccacc agtcaggctg aataagatta ctctgctttc tcaacaaaga taccgtcctg
    17161 atgccctgct tcattaaaga accagatgcc gagcgggtag tcttccagcg aaaccaggta
    17221 cattgtgcct tcactaaact cctcaactgc cagtaccacg ccaggacgac gcggaccgcc
    17281 atccgttttg actgttaccc gatcattcac cttcatcgtt ttcctcctgt ggctttgtgc
    17341 cagtgtagaa caatttcgtt tttctggcag cgccaggcgc gcgcgagtgc tgattttctc
    17401 gacggtctat acttaagaga tgccaggcgg acttaacaac tggcggcaac aacagagtaa
    17461 cggttgcgag gaaagatgat gaaaaccgca aaagagtaca gcgataccgc aaaacgtgag
    17521 gtcagcgtcg atgtcgatgc tctgctggcg gcgatcaatg aaattagcga aagcgaagtt
    17581 catcgcagcc agaacgattc tgaacacgtt agcgtcgatg gacgtgaata tcatacatgg
    17641 cgtgaattgg cggatgcctt cgaactggat attcatgact tcagcgtctc tgaagtgaat
    17701 cgttgaatgc acaataaaaa aatcccgacc ctgagggggt cgggatgaaa cttgcttaag
    17761 caagaagcac ttaaaaaatt cgttacacca ggaaatctga tgtgttcatc accttatctg
    17821 caattttttt cgctgacaag aaaatattcg cgctatgaat gattaacgct acttatgaat
    17881 agaaatgtga cgcgcgtcac cccttggtgg ctctggtgtc aacgggatcg ctcccgaaaa
    17941 aatcatcttt gattatttgt gctgttttga gaatcatcct ggtaagggga gtactgcgcg
    18001 accatgtttt caattcaaca accactactg gtttttagcg atcttgatgg caccctgctg
    18061 gacagtcata gttatgactg gcaaccggca gccccctggc tcagccgttt acacgaagcc
    18121 aatattcccg tcattctctg tagcagtaaa acttcagcgg aaatgctgta cttgcaaaaa
    18181 atgttggggc tacaaggctt accgctgatt gcagagaatg gcgcagtgat ccagcttgct
    18241 gaacaatggc aggatataga cggttttcca cgcatcatct caggtattag ccatggcgaa
    18301 atctgccagg ttttaaatac gctacgcgag aaagaacatt ttaaattcac gacttttgat
    18361 gatgtcgacg atgcaaccat tgccgaatgg acgggattaa gccgtagcca ggcggcgctg
    18421 acgcagctgc atgaggcgtc ggtaacgcta atctggcgcg acagtgacga gcatatggca
    18481 caatttatcg ctcgtctgaa cgaactgggc ttgcagttta tgcagggtgc gcgcttctgg
    18541 cacgtactgg atgcctctgc cggaaaagat caggctgcca actggattat cgcgacctat
    18601 caacaattgt caggcagacg cccaaccaca cttggcctgg gcgatgggcc aaacgatgcg
    18661 cctttactgg aggtaatgga ttacgcggtg attgtgaaag ggctaaaccg tgaaggggtc
    18721 catctgcatg atgaggatcc ggcccgcgtc tggcgaacgc agcgtgaagg accggaaggc
    18781 tggcgtgaag ggctggacca ttttttcccc gcccgttaag cgttatcgct cgcgaatacc
    18841 cgattgcgcc cggcctgttt tgcgaggtaa agccgacggt cggccaatga ctggagttgt
    18901 tcaaaatcat aatcaccagt ttcctcgctg ctgcttaccc ccagcgaggc actgatgcgt
    18961 atcgttgtac tcttggcgat cagcatctct ttttcattta acttcaggcg aatacgttct
    19021 gcgacctccg cagcctgcgt cagattcgcg cctggcagaa tcacacaaaa ctcctcacca
    19081 ccgacccgcc cggcaacgtc ctgcgcacgt aaggaactgc taattaatcc ggcagcatga
    19141 gaaagaacac ggtcgcccac ctgatgacca aagcggtcat taatcgcttt aaaatggtca
    19201 agatcgacct gaatgacaga aaaaggatgt tggtgcgtct gacacaattt agcgagcgga
    19261 caggcttttt cgaacaatgc gccacggtta tataaacgcg ttaaggtgtc gtgccacgcc
    19321 tgccactgca acgagctttg cagaacatac atgttgctga ccatccggcg aatcacatac
    19381 caggagagga gtaacatgct ggtaaagagc gcccacagca gggttaatgc aatgctgata
    19441 ctgccgaaat cgccgcgcac gccttcgctt agcgtatgga cgcgcaccag cacaccgtcg
    19501 aaatggtcca gacgttccca gctaacatag cgactgtcca tacgaatgcc gccccgcgtg
    19561 tcatgttcca ttgcctgcgc cagcaaggcc agttcacgag gatcaaaaat attccctgtt
    19621 ggatggtcag gattggaaga ggtcaaaaat ctcagcttac tgtcatagag ctgatactca
    19681 ccatcgaggt ttttatcgat ggcgtttctt aaaaattgct gcatagtacg cacgggaata
    19741 ctcatcccca gcacgccata ccagtagtta ttactgtcta ccggaacact gacggtaacc
    19801 tgcggttcag tattgctggc gtgttccggt tgcgaagtga accagcgtac cgcgcggtga
    19861 cgattttctc gttgcgaatg gccgataaac cagggttggg tgacatagcc gtaataacgc
    19921 gttggtacat tgcgcgtaaa caaggtcggc tgcgtcgaaa cgtaaaatcc agcacgcgag
    19981 acatacatcg cctgttcaac catcgacgag gagttgtgcg ccagtcgcag caagtaacca
    20041 acttccagtg cagcggtaat ttcattgtcg aggctttcat tttcgcgaga caggagattc
    20101 ccctcgctga ctaatgcatc cgatacgcca ttgactggca gggtgcgccg tcggttgagt
    20161 tcgatttgcc aggcgtgctc gtcgcgatgc tgttcgaact cggttacggc attacgcaga
    20221 gagatgaaat ccagtggcgc cacgagagct tcgcgcatgc cattacgcaa aaagatcagt
    20281 ttgtcgacgt tatactgcaa atgcttatcg agcgcgttgg ctacgttttc cagatgatta
    20341 cgctggctgg agatataggc atcttccagc accaccactt cacgccaggt gagcaaggta
    20401 gaaaaaagca ataccacaat aaagcagaga ttaacgacat gaccaggccc caggcggcgt
    20461 gcgagttttt tcaaccagct ctggttttcc atttttgtct cgtgctgcac cctgacgact
    20521 cccaacctgc ttttttatga ttctggcata acccggcgat agcgtaccgc aaaacagcga
    20581 taattttata atggtggcat gataagacgc gactatcatt cattgcaggc attgggacac
    20641 caggccggat gtgatccggc ctggtgttaa tgataccctt tcaaacttct tccgcagaac
    20701 gactcccctc ccccggaaca agatcggttt catgaaaagc ttcccgcttg accccgtaac
    20761 cgtcatacca acgacactca accataccgc tggagtatcc agtgacaatc atccgcgggc
    20821 cgccctcttt aaccgtaact tcctcactaa ccataaagct catactcgcc tccttttttc
    20881 gagtgaaact tgttcacctt agttgaagat ggcgaatttt gcataatagc cagtgcgagt
    20941 attaatgtgc ctgaccgcgc attttcgcta ctgctttcac tcccagcacc acgatgccgc
    21001 cgatgataaa tccaagaatc agatttaaaa cagtcggtaa tatcatcgcc accactgcac
    21061 tttgctgccc ggcgaaatgt tcaatggcgt gatgcagcgg cgcaatacca tgtaccacaa
    21121 tcccgccgcc gacgaggaac atcgccagcg tgccgacaat cgataatgct ttcatcagcc
    21181 agggcgcgat aatcaataac cctttaccta atgcctgcat cagcgcgctg gatttttccg
    21241 ccagccaata ccccaggtca tcaatcttaa cgataacccc taccagaccg tagacgccca
    21301 cggtcactac cagcgcgatg cctgaaagca ccagcacctg attaagcaac ggcgcttctg
    21361 ccacaatccc cagcgtgatg gcgacgattt ccgcagacaa aataaaatcg gtacgaatcg
    21421 cccctttaat tttgtccttt tcaaacttca gcggatcctg cgccgccagc ttctccagac
    21481 gctgctggct ctgcgctgga tcttctttat gtttacgcgc ctccagcata tgcagcactt
    21541 tctctacccc ttcaaagcag agaaacgcgc caccaatcat caacagtggc gtaatcgccc
    21601 acgggataaa cgcactgatg atcagcgcca gcggcaccag aatcacttta ttgatcagcg
    21661 atcctttcgc cacgccccag accacgggaa gttcccggtt ggcccgcacg cctgaaactt
    21721 gttgcgcatt gagcgataaa tcatccccta atacaccggc ggttttcttc gccgccagtt
    21781 tgcccatcac ggagatatcg tccagcagtg tggcgatatc atcgagcaac gtcagtaaac
    21841 tactcccggc caaaagcatt ccctctcatt tttattgttg aagggtgtaa gtatgaagca
    21901 aaaacggcag ctctgatact tgcttcacag gtcaacattt atttaacaat caagtgcaat
    21961 tatatctctc gccagcagaa tgattttcac gtttactatg aaccaccttt ttatttccgc
    22021 cgtgagggat tatgcgtttc cgccagttgt taccgctttt tggcgcgctg tttgcgttgt
    22081 atatcatttg gggctcaacc tattttgtca ttcggattgg cgtggaaagc tggccgccgt
    22141 taatgatggc gggcgttcga ttcctggcag ccggtatttt attgctggca tttttgctac
    22201 tgcgcggaca caaacttccc ccgctacgtc cgctgctcaa tgccgcgctg attggcctgt
    22261 tattgctggc tgtcggtaat ggcatggtga cggttgccga acatcaaaat gttccttccg
    22321 gcatcgccgc cgtagtggtt gcaaccgtgc ccctctttac cctgtgcttc agccgcctgt
    22381 ttggcattaa aacgcgcaaa ctggaatggg tgggtatcgc cattgggctt gccggaatca
    22441 tcatgctcaa tagcggtgga aatttaagcg gcaatccgtg gggcgcgatt ctgattttaa
    22501 tcggctcgat tagctgggcg tttggctcag tttatggctc gcgcattacc ttacctgtag
    22561 ggatgatggc gggtgcgatt gagatgctgg cggcaggcgt ggtgttaatg atcgcgtcga
    22621 tgattgcggg tgaaaaactg acggcgctcc cttccctgtc aggcttcctt gcggtcggct
    22681 atctggcgct gtttggttcg attatcgcca ttaacgctta tatgtattta atccgtaatg
    22741 tcagtccggc tctcgccacc agctacgcct acgttaaccc ggtggtcgcg gtcttgctgg
    22801 gtacgggact gggtggcgaa acgctgtcga aaattgaatg gctggcgctc ggcgtaattg
    22861 ttttcgcggt ggtactggtc acgttgggaa aatatctctt cccggccaaa cccgttgtcg
    22921 cgccagttat tcaggatgca tcaagcgagt aaatgaatcc cctgcgtgtc gatctgcgcg
    22981 ctggcacctt cgccgcagat ccactcttcc agacgctcgg taagcgccgc atccgtcagc
    23041 ttctcgcgcc cacgtaacgc acactcccag acaatcagta cgcgccagcc gagctcctgc
    23101 aagcgactga tatcgcggcg atcgcgttca acatttttac ctatcttctc cagccagaac
    23161 tcagttcgag tcgcaggcac tttaaacaga tagcagtgat gatgatgcca gaagcagccg
    23221 tgggtaaata tcacgcagcg atattcatca acgacaaaat ccgggcgtcc gggcagactg
    23281 gcgtcctgaa cgcgaaacgc aaggccctgc ccggttaaca aactggcgag gcgcttctca
    23341 atcgccgtat cacgcgtggc aatcgcgcgc atatttttgc tgcgggtggc cttatcgtga
    23401 acgtcgacca tgttgtgcct cttgctgacg caacgccacc gcctgtttga tttttggctc
    23461 aagcagtttt gccaccgcgg caaagaccag cacgaccacc gagttaccga actggcgata
    23521 ggcctgagtg tccgaaaccg gaatgcggaa tttcgcttct cccggcgctt caaaacccat
    23581 taagcgcgcg cattcacgag gcgttaaccg acgtggacga tgttgctgat tcagcggatc
    23641 gtcaaagtct ttctcacccg tggccatatc ccaaccgcga tcgattaaaa tttccgcgcc
    23701 atctttgtaa taacgcgcag acagcgtacg ggtgacgctt tgcggattgt tcggataaac
    23761 cattccataa ccgaagccgt taccgcgcgc ctgatgtttt ttcgcatatc gatagaggta
    23821 cttccacagc accggcgtca ggatatattt tgcctcaacc atcgggtcca gcagctgcgc
    23881 cagcgtcact cgctgtgcag ggaaacattc gctgatatca cgcagagtaa aatcggcttt
    23941 cagattcaga tcgcgacgaa aacccaccag cacgatgcgt tcacggtgct gcggcaggaa
    24001 atgtttgccg tcgatgattt tcggatcgtc cggcccgtta tcttctgcat cagccacgtc
    24061 atagcccagt tcgtccagcg tctgcatgat gatgcggaac gtttttccct tatcgtgact
    24121 cttcaggttt ttgacatttt cgagcacaaa catcgccgga cgacgcgcgt cgataatgcg
    24181 caccacatca aagaacagcg tgccctgggt atcgcaggca aaaccgtgcg cccgcccgag
    24241 cgagttcttt ttcgatacgc cagccagcga aaatggctga caagggaaac cggccagtaa
    24301 aacatcgtgt tcaggaatgt gttgacgaat atgttccgcc gccgcctcat cactcacgcc
    24361 ttcttgatgg ctgagggtga tgtcgcggat atcttcatta aaatgatgcg tcgccgggtc
    24421 gcaataatgg ttggctttat aagtgcgtac cgcatgtttg ttccattcgc tggtaaacac
    24481 gcactgtcca ccaatcgatt caaaaccgcg acggatgccg ccaattccgg caaatagatc
    24541 gataaagcga aacgcataat gcggatgatg ttccggtggt ttgggtaaca acgtttgcag
    24601 atgggcaaac tctttctcac ttaaacggtg ccatgccgag tcattcgcca gcgcacgttt
    24661 taaaattgcc gcgctccagt gattctcacc cacaccatta agctgcgcca ccagcgtttt
    24721 aacatcataa atttgcagca gtttctccag cattgcctgt gcggcattcc cggtgctgta
    24781 tgaatcggtt actgatatat tttcctgcat agatttaacc ggccatctga gatggccgac
    24841 aggttaacat aattactgcc gtttagggcc acgcgctcag ccagcgggtg aaaacgcatc
    24901 tatcaccttg tgatcgatac cttcattctc ccccgccagt tcggcaccga gcttcgccat
    24961 aaactcgact aaaaattgcg cgttgtgctg tgccagctgc ttgcctcttg cggtttgcat
    25021 ggtttgcggt aatttcagta actttgtctg gaaatgatcc agcgcgtagc gtttatcatc
    25081 aagcgggcga tgctgtgcga acggatcttc gccatcaaac agcgccaagc ccaatgcccc
    25141 tgaaacggca aatacgcgcg ccaggccaat cgcccccagg gcttccagcc gatccgcatc
    25201 ctggacgatt ttagcttccg ttgttaaggg ggcgatttgc gcgctgaaac tgtgagcggc
    25261 aatggcatga caaacggcct caattttctc cgccggaaat tgcgcgaact cttcacggag
    25321 caggcgacgc gtctcttctg ccgccaggat tgaagaacgc tgccgttgcg gatgattttt
    25381 cgccaggctc acgatatcgt gaaaataaca ggcggttaaa atcaccagca tatcgacatc
    25441 gtcatctgcc gccagtttct gtgccgtagc ccagacacgg cgaaaatgac acaggtcatg
    25501 cgccgcgtcc tgatgttgat gatggttttt caaccagttc tcgaactgtg cctgccagtg
    25561 ttgtaactcc atacgcgctc cgttgccaaa gagtcgcaac cttagcagtt tttattcata
    25621 gcgtctgcaa cgtgcgggac gaggtcgggt gtaccatgca atgccgccta acaaggcacc
    25681 aaccagggcc ataaacagaa atccgaccaa tgctcctaac cacttccccg cggtagatcc
    25741 taaatcactt tcaaatcccg acacgggggc atcggggatc cggctcatca cccagatgta
    25801 tcgcgccatt gcccagacga aatagccaca ccatgtatag aaagcccaca aagccaattt
    25861 gccgccaggg ctgcgagaga gtctctcttc catctttgtc gaaccgtatt ccattttgag
    25921 ataacccgga aaattcagac ttagaactat tatgtgataa aaatcaca
//
LOCUS       DAHHTG010000064        25947 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.064, whole genome shotgun sequence.
ACCESSION   DAHHTG010000064 DAHHTG010000000
VERSION     DAHHTG010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 25947)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25947)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25947
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.064"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            complement(1..730)
                     /locus_tag="NBI74_002569"
                     /pseudo
     CDS             complement(1..730)
                     /locus_tag="NBI74_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000378101.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase"
     gene            808..1107
                     /locus_tag="NBI74_002570"
     CDS             808..1107
                     /locus_tag="NBI74_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001332034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCD1932887.1"
                     /translation="MLGVLTLARDIQLAIVNKMTQVLIGDIYLRQQCDVFWLGYTISP
                     AYTRQGYAIEAITATIDWIKENGFSLIKAGVNPENTLSKKLLIRIGFNFSSIEDG"
     gene            1484..1741
                     /locus_tag="NBI74_002571"
     CDS             1484..1741
                     /locus_tag="NBI74_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjhX family toxin"
                     /protein_id="HCD1932888.1"
                     /translation="MNLSRQEQRTLHVLAKGGRIAHVRDSSGRVTSVECYSREGLLLT
                     DCTLAVFKKLKTKKLIKSVNGQPYRINTTGLNNVRAQLDNR"
     gene            1753..2304
                     /locus_tag="NBI74_002572"
     CDS             1753..2304
                     /locus_tag="NBI74_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014837489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HCD1932889.1"
                     /translation="MTAHHFTFQITDESDASDIREVETRAFGFSKEADLTASLLEDES
                     ARPALSLLARHEGKAVGHILFTRATFKGEMDSPLMHILAPLAVIPEYQGMGVGGRLIR
                     TGIEHLRLMGCQTVFVLGHATYYPRHGFEPCAGDKGYPAPYPIPEEHKACWMMQALTA
                     QPMNVTGHIQCAQALMKPEHWRE"
     gene            2356..3102
                     /locus_tag="NBI74_002573"
     CDS             2356..3102
                     /locus_tag="NBI74_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCD1932890.1"
                     /translation="MDIPRIFTISESEHRIHNPFTEEKYATLGRVLRMKPETRILDLG
                     SGSGEMLCTWARDHGITGTGIDMSSLFTAQARRRAEELGVSERVHFIHNDAAGYVANE
                     KCDVAACVGATWIAGGFAGTVELLAQSLKPGGIMLIGEPYWRQLPATDEIAQACGVSS
                     TTDFLTLPGLVSAFDDLGYDVVEMVLADQEGWDRYEAAKWLTMRRWLEANPDDDFAAE
                     VRAELNISPKRHVTYAREYFGWGVFALIAR"
     gene            3230..3490
                     /locus_tag="NBI74_002574"
     CDS             3230..3490
                     /locus_tag="NBI74_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000084876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1932891.1"
                     /translation="MSQHEQFCQACGMPMSAPDAHSASDQYCAYCSDSNGNLKPWEEA
                     VSGLASFLDSWQKVGPEEAHKRAKRYLTAMPAWAHKAESDLS"
     gene            3528..3644
                     /locus_tag="NBI74_002575"
                     /pseudo
     CDS             3528..3644
                     /locus_tag="NBI74_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227376.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
     gene            3889..5010
                     /locus_tag="NBI74_002576"
     CDS             3889..5010
                     /locus_tag="NBI74_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418725.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HCD1932892.1"
                     /translation="MSFSVQETLFSLLQHNAISGHENAVADVMLCEFRRQAKEVWRDR
                     LGNVVARYGSDKPDALRLMIFAHMDEVGFMVRKIEPSGFLRFERVGGPAQVTMAGSIV
                     TLTGDKGPVMGCIGIKSYHFAKGDERTQSPSVDKLWIDIGAKDKDDAIRMGIQVGTPV
                     TLYNPPQLLANDLVCSKALDDRLGCTALLGVADAISTTELDIAVYLVASVQEEFNIRG
                     IVPVLRRVKPDLAIGIDITPSCDTPDLHDYSEVRINQGVGITCLNYHGRGTLAGLITP
                     PRLIRMLEQTALEHNIPVQREVAPGVITETGYIQVEQDGIPCASLSIPCRYTHSPAEV
                     ASLRDLTDCIRLLTALAGMSAAHFPVEPDSGTTQEAHPL"
     gene            5007..5285
                     /locus_tag="NBI74_002577"
     CDS             5007..5285
                     /locus_tag="NBI74_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HCD1932893.1"
                     /translation="MKKILVACGTGMSTSTMIAHKLQEFLTEQGISATTAQCCLNEIP
                     LNCNGMDLIVTSMRTNSDYGIPTLNGAALLTGINDDALKQQIKALLTQ"
     gene            5297..6610
                     /locus_tag="NBI74_002578"
     CDS             5297..6610
                     /locus_tag="NBI74_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HCD1932894.1"
                     /translation="MFDYILSLGGTVFVPIIMIVIGLIFRIPWLQAIKAGVTVGIGFV
                     GMGLVIVMAIDSLSPPIKVMIERFGLALHVFDVGAGPASGVGYATAIGAMIIPVIFLL
                     NVAMLVTRLTKTMNVDIYNYWHYAITGTVVQLMTGSLIYGVLGAICHAALSLKMADWT
                     AKRVQNIVGLEGISIPQGYGSSSVPLFVLLDAIYEKIPFMKGRNIDAQEIQKRYGMVG
                     DPVIIGVVLGLIFGLAAGEGFKGCASLMITVAAIMVLFPRMIRLIVEGLMPISDGARK
                     FFQKYFKGREVYIGLDTAVTLGHPTTIAVGLLLIPIMLILASILPGNKVLPLADLPVA
                     PFFICMATVIHRGDLVRTLISGVIVMITVLLIATQFAPYFTEMALKGGFSFAGESAQI
                     SALSVGNMFGWSISELMSLGIIGVVVAVGIVASVVLFLRKRELSE"
     gene            6623..7429
                     /gene="sgcQ"
                     /locus_tag="NBI74_002579"
     CDS             6623..7429
                     /gene="sgcQ"
                     /locus_tag="NBI74_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BtpA family protein SgcQ"
                     /protein_id="HCD1932895.1"
                     /translation="MSWLKEVIGTEKAVIAMCHLRALPGDPSFDAQLGMNWVIDKAWD
                     DLMALQNGGVDAVMFSNEFSLPYLTKVRPETTAAMARIIGQLMSDIRIPFGVNVLWDP
                     VASFDLAMATGAKFIREIFTGAYASDFGVWDTNVGETIRHQHRIGAGEVKTLFNIVPE
                     AAVYLGNRDICSIAKSTVFNNHPDALCVSGLTAGTRTDSALLKRVKETVPDTVVLANT
                     GVCLENVEEQLSIADGCVTATTFKKDGVFANFVDQARVSQFMEKVHHIRR"
     gene            7560..7991
                     /locus_tag="NBI74_002580"
     CDS             7560..7991
                     /locus_tag="NBI74_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HCD1932896.1"
                     /translation="MINDIKWVQAQREATDWRQAVEIATRPLVAYGAAQPCYVNGIIE
                     NTLNWGPYYLIAPGIALPHARPEQGANYNQVSITTLRTPVAFGNEECDPVWLLLCVSA
                     TDANAHILTIQRISQFIDSPQRLAAVGNASTDDALFALVSG"
     gene            8003..8635
                     /locus_tag="NBI74_002581"
     CDS             8003..8635
                     /locus_tag="NBI74_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3 epimerase family protein"
                     /protein_id="HCD1932897.1"
                     /translation="MILHPSLASANPLHYGRELTALDNLDFGSLHLDIEDSSFINNIT
                     FGMKTVQAVARQTPHPLSFHFMVARPQRWFNALAEIRPAWIFVHAETLDYPSETLTEI
                     RHTGARAGLVFNPATPIDAWRYLASELDGVMVMTSEPDGQGQRFIPSMCEKIQKVRTA
                     FPQTECWADGGITLAAAQQLAAAGAQHMVIGRALFSSSDYRATLAQFATL"
     gene            8652..9434
                     /locus_tag="NBI74_002582"
     CDS             8652..9434
                     /locus_tag="NBI74_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCD1932898.1"
                     /translation="MSQQRPDRIKQMLHYLWQHRHLSTQQAMELFGYAEATVRRDFQY
                     IANQYPGMIRGHGCLDFDDSTDDKEYVFDVKRTLQSVAKREIAALARTMIKDGDCFFL
                     DSGSTCLELAKCLADARVKVICNDIKIANELGCFPHVESYIIGGLIRPGYFSVGESLA
                     LEMINAFSVERAFISCDALSLETGITNATMFEVGVKTRIIQRSREVILMADHSKFDAV
                     EPHAVATLSCIKTIISDSGLPETIAQRYQRAGCQLFLPHSIK"
     gene            9737..10525
                     /locus_tag="NBI74_002583"
     CDS             9737..10525
                     /locus_tag="NBI74_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HCD1932899.1"
                     /translation="MVRKGCNSLVRAEKILTHIAWVGMASYMELLNKFQYPKSSLLNL
                     LNVMVECGFLIKNKNGYYSLGIKNYELGCQALHRQNIFEVTKRPMQELSLKSGLVCHL
                     GAMESISAIYLDKIESPDSVPTSKSWIGKKLELHITALGKALLAWKTREELDYFLEAL
                     TLTPHTRNTFTDKKLFLEELQKTRLRGWAIDNEESTYGAVCLSMPVFNMYNRVNYAIS
                     LSGDPVVYSGNKIDSYLELLRKCAEQISYGLGYRNENEHLRKGN"
     gene            10530..11435
                     /locus_tag="NBI74_002584"
     CDS             10530..11435
                     /locus_tag="NBI74_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HCD1932900.1"
                     /translation="MKKFSGIIPPVSSTFHRDGTLDKKAMREVADFLINKGVDGLFYL
                     GTGGEFSQMNTAQRMALAEEAVTIVDGRVPVLIGVGSPSTDEAVKLAQHAQAYGADGI
                     VAINPYYWKVAPRNLDDYYQQIARSVTLPVILYNFPDLTGQDLTPETVTRLALQNENI
                     VGIKDTIDSVGHLRTMINTVKSVRPSFSVFCGYDDHLLNTMLLGGDGAITASANFAPE
                     LSVGIYRAWREGDLATAATLNKKLLQLPAIYALETPFVSLIKYSMQCVGLPVETYCLP
                     PILEASEEAKDKVHVLLTAQGILPV"
     gene            11446..13413
                     /gene="yjhG"
                     /locus_tag="NBI74_002585"
     CDS             11446..13413
                     /gene="yjhG"
                     /locus_tag="NBI74_002585"
                     /EC_number="4.2.1.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylonate dehydratase YjhG"
                     /protein_id="HCD1932901.1"
                     /translation="MSVRNIFADESHDIYTVRTHADGPDGELPLTAEMLINRPSGDLF
                     GMTMNAGMGWSPDELDRDGILLLSTLGGLRGADGKPVALALHQGHYELDIQIKAAAEV
                     IKANHALPYAVYVSDPCDGRTQGTTGMFDSLPYRNDASMVMRRLIRSLPDAKAVIGVA
                     SCDKGLPATMMALAAQHNIATVLVPGGATLPAKDGEDNGKVQTIGARFANGELSLQDA
                     RRAGCKACASSGGGCQFLGTAGTSQVVAEGLGLAIPHSALAPSGEPVWREIARASARA
                     ALNLSQKGITTREILTDKAIENAMTVHAAFGGSTNLLLHIPAIAHQAGCHIPTVDDWI
                     RINKRVPRLVSVLPNGPVYHPTVNAFMAGGVPEVMLHLRSLGLLHEDVMTVTGSTLKE
                     NLDWWEHSERRQRFKQLLLDQEQINADEVIMSPQQAKARGLTSTITFPVGNIAPEGSV
                     IKSTAIDPSMIDEQGIYYHKGVAKVYLSEKSAIYDIKHDKIKAGDILVIIGVGPSGTG
                     MEETYQVTSALKHLSYGKHVSLITDARFSGVSTGACIGHVGPEALAGGPIGKLRTGDV
                     IEIKIDCRELHGEVNFLGTRSDEQLPSQEEATAILNARPSHQDLLPDPELPDDTQLWA
                     MLQAVSGGTWTGCIYDVNKIGAALRDFMNKN"
     gene            13520..14869
                     /locus_tag="NBI74_002586"
     CDS             13520..14869
                     /locus_tag="NBI74_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
                     /protein_id="HCD1932902.1"
                     /translation="MPLIIVVAGIALLLLLTIKIKLNTFVSLIIVSIAVAIASGMDLS
                     KVVTSVESGLGGTLGHIGLIFGFGVMLGRLLADAGGAQRIALTMLNYFGKNKLDWAVV
                     CSAFIVGIALFFEVGLILLVPILFAIAREAKISPMFMCVPMLSGLLVAHGFLPPHPGP
                     TVIAREYGADVGLVLIYGIIVGIPTFILCGPVLNKFCQRIIPDAFKKEGNIASLGATR
                     RFSESEMPGFGISFLTAMLPVILMAVVTIIQMTHAKSAADSGLFYNVILFLGNSTIAM
                     LISLLFAIYTMGLGRGKTIPDLMDSCGKAIAGIAGLLLIIGGGGAFKQVLIDSGVGQY
                     ISTLVSGMDINPILMAWGVAAFLRICLGSATVAAISTAGLVIPLLAVHPNTNLALITL
                     ATGAGSCICSHVNDASFWMIKDFFGLTTKETLLSWTLMSTLLSISGLIFILLASLVL"
     gene            15216..16202
                     /locus_tag="NBI74_002587"
     CDS             15216..16202
                     /locus_tag="NBI74_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418715.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HCD1932903.1"
                     /translation="MDRDQTNSSLFNDDPVLHATWLYYQEGKSQTEVAAIMGVSRVTV
                     VKYLQTARENGLVHINLDVNVFGSIDAALQIRDKFNLQRVIIVPDGEHAGKRDDTKLM
                     RTRLSRAGGMYLNQVIENGDVLGVAWGRTIHQMSKTMTPKSCKNVTVIQMLGSMPSQP
                     DLTIIESSSQIAYKLSGRVASLHVPAVVSSARLAMELQAEPIIRSNFDVLTRCTKAFF
                     VVGNALDENPLIRVGVLNKKEMQTYRDLGAVGVICGRFYDKEGMPVVADVDQRILGIS
                     LAQLRQIERKIFLAGGERGYDATLGALLGGYVTDLIVDEGTAEFLLACELPH"
     gene            complement(16243..17247)
                     /locus_tag="NBI74_002588"
     CDS             complement(16243..17247)
                     /locus_tag="NBI74_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001622580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug DMT transporter permease"
                     /protein_id="HCD1932904.1"
                     /translation="MFIVESYAVAIIMCFITMICWGSWANTTKLVSNKKWEFPLFYWD
                     YSIGLLLCSLLFAFTLGSMGEAGRSFIPDIQQASSSSLMSAILAGIIFNISNILLVAS
                     INLAGMAVAFPVGVGLALALGVITTYIGNPQGDPLILFLGVACVVIAIIFTAIAYGRV
                     TQEADKSRRNKGLITAILAGIIMGWFFRFLADSMSDNFSQPASGLMTPYSALVLFAVG
                     LFLSNFVLNTLVMKKPISGEPVNGKMYFSGSLRDHVCGWLGGMIWCVGLAFSLIASGQ
                     AGYAISYGLGQGATMIAVIWGVFIWREFASAPAGTNKLLLTMFISYIVGIVLIIAANQ
                     "
     gene            complement(17351..17992)
                     /locus_tag="NBI74_002589"
     CDS             complement(17351..17992)
                     /locus_tag="NBI74_002589"
                     /EC_number="4.1.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000439687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculose-phosphate aldolase"
                     /protein_id="HCD1932905.1"
                     /translation="MERIKLAEKIISTCREMNASGLNQGTSGNVSARYTGGMLITPSG
                     IAYSKMTPDMIVFVDDKGIPEAGKIPSSEWLFHLACYKARPELNAVIHTHAVNSTAVA
                     IHNHSIPAIHYMVAVSGTDHIPCIPYYTFGSPELADGVSKGIRESKSLLMQHHGMLAM
                     DVTLEKTLWLAGETETLADLYIKCGGLHHDVPVLSEAEMTIVLEKFKTYGLKA"
     gene            complement(18023..19138)
                     /gene="mtnA"
                     /locus_tag="NBI74_002590"
     CDS             complement(18023..19138)
                     /gene="mtnA"
                     /locus_tag="NBI74_002590"
                     /EC_number="5.3.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001022014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5-thioribose-1-phosphate isomerase"
                     /protein_id="HCD1932906.1"
                     /translation="MNIKGKHYRTVWVSGDGKAVEIIDQTKLPFKFEVVALTSAEMAA
                     TAIQEMWVRGAPLIGVVAAYGIALGMNHDASDMGLQRYYDLLIKTRPTAINLKWALDR
                     MIDTLKDLCVSERKDVAWALAAEIAEEDVALCEQIGLHGTEVIREIAQKKPAGSVVNI
                     LTHCNAGWLATVDWGTALSPIYKAHENGIPVHVWVDETRPRNQGGLTAFELGSHGIPH
                     TLIADNAGGHLMQHGDVDLCIVGTDRTTARGDVCNKIGTYLKALAAHDNHVPFYVALP
                     SPTIDWTIEDGKSIPIEQRDGKEQSHVYGINPQGELSWVNTAPEGTRCGNYAFDVTPA
                     RYITGFITERGVCAASKSALADMFADLKSKALQGEQH"
     gene            complement(19148..20407)
                     /locus_tag="NBI74_002591"
     CDS             complement(19148..20407)
                     /locus_tag="NBI74_002591"
                     /EC_number="2.7.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000132329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5-thioribose kinase"
                     /protein_id="HCD1932907.1"
                     /translation="MTDSIPSGYKPLTCDTLPGYLSSRLTPSCEPGGLPEEWKVSEVG
                     DGNLNMVFIVEGTHKTIIVKQALPWLRAGGEGWPLSLSRAGFEYNVLCQEAKYAGHTL
                     IPQVYFYDPEMALFAMEYLTPHVILRKELINGKKFPKLAEDIGRFLAQTLFNTSDIGM
                     SAEQKKALTAEFALNHELCKITEDLIFTEPYYNAERNNWTSPELDDAVHKAWADVEMI
                     QVAMRYKYKFMTEAQALLHGDLHSGSIMVTDTDTKVIDPEFGFMGPMAFDIGNYIGNL
                     LLAYFSRPGWDANEQRRADYQEWLLQQIVQTWSVFTREFRQLWDNKTQGDAWPTEMYQ
                     QNRAALEDAQDQFFATLLEDSLVNAGMEMNRRIIGFAGVAELKQIENTELRAGCERRA
                     LTMARDLIVNARQFKNMDSVIQSAKVK"
     gene            21207..21461
                     /locus_tag="NBI74_002592"
     CDS             21207..21461
                     /locus_tag="NBI74_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000788354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HCD1932908.1"
                     /translation="MKNGIDVSVIIPAYNAAETSVSLVTKILSEPHLAIELIIVDDGS
                     TDETGNVLRQIHDDRLILIEQANQGVYAARNAALALHQGE"
     gene            22565..24640
                     /locus_tag="NBI74_002593"
     CDS             22565..24640
                     /locus_tag="NBI74_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000177022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCD1932909.1"
                     /translation="MDLVDSVEAGKLTISELIDALAKDKNYSASKWDQRYREFTTLLQ
                     QTSTFSEPETDGLVKRLWYERDNGIASIRQGVPSLAEYQQSLPLLRELTERIRQQPDE
                     ETYQYVGNALQQAKENGLLKRMYRSLRNRVFAAFSPENYTSTVDENAFSKAAEFLNQH
                     FHLGLALTGNWLQKNYELKQAIHAQSPDTDPYYVNMAIWHLYELLRERDNEQKQEKIA
                     STTFITSNEPIENKIILHSPTNVIFFGPPGTGKTFRLQQKMKEYTSHAVPADRDAWLD
                     SRLESLNWMQVITLVLLDLGKRAKVRQIIEHMWFQRKALLNGRNGNLSNTAWAALQSY
                     TVPESLTVDYKNRREPAVFNKTDNSEWLLVDSQLEQVEDLVELYAELKRGPKSAEAIQ
                     RFAVVTFHQSYGYEEFIEGIRARSDESGNISYPIEPGIFMRLSQRANADPGHRYAIFI
                     DEINRGNISKIFGELISLIEVDKRAGMPNAMSLQLAYSGDHFSVPANVDIIGAMNTAD
                     RSLALMDTALRRRFDFVEMMPDLSLLSGAKVKGIELESLLEKLNSRIEALYDREHTLG
                     HAFFMPVKNALDAGDEEAAFKQLKIAFQKKIIPLLQEYFFDDWNKIRLVLADNQKQDD
                     NLQFVIEKTDDLDTLFGNNHGLRHHDQQSTAYELKDFDQEIWNIPQAYRSIYQPQQTP
                     LDEQAVNHG"
     gene            24633..25919
                     /locus_tag="NBI74_002594"
     CDS             24633..25919
                     /locus_tag="NBI74_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000504875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="McrC family protein"
                     /protein_id="HCD1932910.1"
                     /translation="MGEVISVFEYDLLGSDKAASVGAKLVPPLVFNYLEALSLASNQG
                     SQFLKLTSRSGFKLLQVQNYAGMLSTPHGFQLEILPKVGKNLTAANARQTLLTMLSHL
                     PGFRHIETQQATLQAQRMPLLEIFIHQFLHSVSQLLKQGLRSDYVSEQGNLAFMKGKL
                     MLSAQLRHNAVNRHKFCVDYDDYMPDCAANRLLHSALDKLLSLKLSSENQRWLYELRF
                     AFDGIPLSRDIERDINNLRLERGMAHYNEPMAWAQLILRGMSPSALQGNTKAISLLFP
                     MEAVFESFVAQTLPYELPSHLKVIPQAATYSLVKHGSNDCFKLRPDLLIQSRRPVQTK
                     LVMDTKWKLINSNSQKTTQYGLAQADFYQMFAYGQKYLNGIGEMYLIYPAHDQFNQPI
                     QQHFAFSKTLKLWVVPYQITANRGERMMWVKDVSSI"
BASE COUNT         6683 a         6390 c         6312 g         6562 t
ORIGIN      
        1 ttaaatcctg tcgtttctga aactcaaatt gcaacgcatt attatctgaa gaaaagagat
       61 attttgccaa taaccctcgc ggcgaaaatg cgggctgcat ctcgataagg tccatatcgc
      121 caaataccag aaaactatct ttagcgcgtg agacagcaac attgaggata ctgctgttgc
      181 tatcaagaaa cctgccatct tcatgttttg agtagacagg agaaaagaga acaatcgccc
      241 tttccgcccc ctgcaaggag tgcacagtcc ccactgtcag tgaaccctgc tcgtctttgc
      301 cattaatttc cagcttacgc aatgacgttt tgatagcatt aacctgcgcc gaaaatggcg
      361 tcacaacacc aacaactttg tacagtggtt ccccgtaatg gcgctctata tcatccttat
      421 gtgccaccag ccatgctgct attgtttcag cttcaagagg gttataacgg cttcctccat
      481 ttggcttcat gcctctacca tcaatatgta aatatcccat tgcggggaaa atcgtccctt
      541 tttccatccc tcttttaggc tgcaacttac cgtgatagca aagtgagtta cagtagccga
      601 tgatgttatt gaaacagcga cgatgctcat acagatacat tccgcgctcg aacttgggat
      661 catactggta gtgagagttg aactgggcta cctgcattac gctgccagat gctgcacttt
      721 ttccagaccc atttattatc gaatacggtg accgcctgag cggccacctt taatcgtggg
      781 cagttacaca gaattattta cagggccttg ctgggagttc ttacacttgc tcgcgatatt
      841 caattggcca ttgtcaacaa gatgacgcag gttttgattg gtgatatcta tttacgtcag
      901 caatgcgatg tgttttggct cggctatacc atcagtcccg cttatactcg ccagggatat
      961 gcaattgaag caattaccgc cacgattgac tggataaaag agaacggttt ttcactcatc
     1021 aaagccggtg tgaatccgga aaataccctt tcaaaaaaat tacttatcag gattggcttc
     1081 aatttttcga gcatcgaaga tgggtgagca tatttatgtt ctggattaaa caaaaggaga
     1141 ataatctccc gaagggaaaa gccgatgagg ccatttcagg cgtacttcaa catgcattcg
     1201 tcagtgtcat ctgccttttc cagtaaatgg gttggcattc aatttccaaa gtacatcaca
     1261 ctttttctac tgaaacatat ccctcctata actacgtgtt aatattcgct ccgtaaatac
     1321 ctttcaactc aacccgaggc tttgatgctg gagaatgtaa tcatccgata atcagcttac
     1381 cgaccttttc aggccggact gattatcaat gcgccgaaat cgaatgcgga caccgcggtg
     1441 tgtttgcacg ttttgcacat gatgattaaa caggttcttc agtatgaatt tatcccgtca
     1501 ggaacaacgt acgttacacg ttctcgctaa aggtggacgt attgcgcacg tccgcgattc
     1561 ttcaggccgc gtcacttccg ttgaatgcta cagccgcgaa gggctgttgc tgaccgactg
     1621 cacgctcgcc gtcttcaaaa aactcaaaac caaaaaactt atcaagtccg tcaatggtca
     1681 gccctatcga atcaacacta ccgggctgaa taacgttcgc gctcagctcg ataatcgcta
     1741 aggagagttt cgatgacagc acaccacttc acttttcaaa tcacagatga aagtgatgcc
     1801 agtgatatcc gggaagtcga aacccgcgca tttggcttca gcaaagaagc cgatttaacg
     1861 gcctctttac ttgaggatga aagcgcgcgc cctgcgttat cgctgctggc ccgacatgaa
     1921 ggcaaagccg tcgggcatat tttatttacc cgggcaacct tcaaagggga aatggattca
     1981 ccgctgatgc atatcctcgc tcccctggca gtcataccgg aataccaggg gatgggcgtg
     2041 ggaggacggt tgatacgcac tggcatcgag cacctgaggt tgatgggctg ccagacggtt
     2101 tttgttctcg gccacgccac ctattacccc cgacatgggt tcgaaccctg tgccggagac
     2161 aaaggctatc cggcacctta tcccatcccg gaggagcaca aagcctgctg gatgatgcaa
     2221 gcactcaccg ctcagcccat gaacgtgacc ggccacatcc agtgcgcgca ggccctgatg
     2281 aaaccggagc actggcgtga ataacaatct tgttgtgccc tttttaatgc ttaacccaat
     2341 aacaggaatt aactaatgga tattccacgt atttttacca tcagtgaaag cgaacaccgc
     2401 atccacaacc cgttcaccga agagaagtac gccacgctgg gtcgcgtgct gcgcatgaaa
     2461 ccagagaccc gaattctgga cctcggcagc ggctcgggcg agatgctctg cacctgggcc
     2521 cgggatcatg gcattacggg gactggaatc gacatgagtt cgctcttcac cgcgcaggcc
     2581 agacgccgcg cggaagaact cggcgtcagc gaacgcgtac attttattca taatgatgcg
     2641 gcgggttacg tcgccaatga aaaatgtgac gtagcagcct gcgttggcgc gacctggatt
     2701 gccggaggct ttgccgggac agtcgaactg ctggcgcaga gccttaaacc gggcgggatc
     2761 atgctcatcg gtgaacctta ctggcgtcag cttccggcaa cggacgagat agcccaggcc
     2821 tgtggcgtca gctcgacgac cgattttctg acgctaccag ggcttgtcag cgcgtttgac
     2881 gacctcggct acgacgtggt ggaaatggta ctggcggacc aggaaggctg ggaccgatat
     2941 gaggccgcga aatggctgac catgcgccgc tggctggagg ccaatcctga cgatgacttc
     3001 gccgccgaag tcagggccga gctaaacatc tcgccgaaac gccacgtaac gtacgcgcgg
     3061 gaatactttg gctggggcgt gttcgcactc atcgctaggt aacaacaggt tcccggtatt
     3121 tctgaccgga gataccgggg gcttagcctt tgctttaatc tgatgcatca cctgcacaat
     3181 acatattcag tatgtttatt aatcaagatg tcaccataag gagcatcata tgtcacaaca
     3241 tgaacagttc tgtcaggctt gcggaatgcc gatgtcggca ccagatgcac acagtgccag
     3301 tgatcaatat tgtgcgtact gcagcgacag caacggcaat ctcaaaccct gggaagaagc
     3361 ggtatcaggt ttagccagct tccttgattc ctggcaaaaa gtcggcccgg aggaagcaca
     3421 taagcgtgca aaacgttatc tgacggcaat gcccgcctgg gctcataaag cggagagtga
     3481 tttgagctga ttatctatac tccctctgaa tctattcagg agatccgatg ttttcttaca
     3541 ttatgctggg aaccaacgac ctgccacgcg ccattacatt ctaagatccg ctgatggaga
     3601 tgctggggta tccacgtgca ggacgtaatg aagagggagc gtcctgcaac aaagtggtgc
     3661 gataacagtt accgcgtttt attcattctc attatttcaa ccataatcac tggtctctct
     3721 agcccgtaac tttcacaaac gttgagcgct tttattccac tcggctcaca taatttttta
     3781 tttgttcagc tttgcgcgtt ttcacgtgaa cgttctcaca attattcgac cagtctgcgc
     3841 ctataaaacg ctcaacgagt gattgtttta cggtctacgg agatagctat gtcattttct
     3901 gtgcaggaaa ccctgttctc gcttttgcag cacaatgcga tttcaggaca cgaaaacgct
     3961 gtcgctgacg tcatgctgtg cgaattcagg cgtcaggcaa aagaggtctg gcgagacagg
     4021 cttgggaatg tcgtcgcgcg ctacggtagt gataaacccg atgcgctgcg actgatgatt
     4081 tttgcgcata tggatgaagt cggttttatg gtgcgcaaaa tagagccttc tggattttta
     4141 cgctttgaac gcgtaggcgg tcctgcgcag gtcactatgg ctggatccat cgtcaccctc
     4201 accggggaca aagggccagt catggggtgt atcggcatta agtcctacca ctttgccaaa
     4261 ggcgacgagc gcacgcagtc accttctgtc gacaaactgt ggattgatat tggtgccaaa
     4321 gacaaagacg acgctatacg aatgggcatt caggtcggta cgcctgtcac tctgtataac
     4381 ccgccgcaac tcctggcaaa cgatctggtg tgcagtaaag cactagacga tcgtctgggc
     4441 tgtactgccc tgctcggtgt agcggatgct atcagtacta cggagcttga tatcgccgtt
     4501 tatctggtgg cttcggtaca ggaagaattt aatatccgcg gcattgttcc cgtattacgc
     4561 cgtgtaaaac ctgacctggc gattggtatt gatatcactc catcgtgtga cacccctgat
     4621 ttacacgatt attccgaggt caggattaat cagggcgttg ggatcacctg cctgaactac
     4681 catggtcggg gaacgctggc cggattaatc acgcctcctc gcctgatacg gatgttggaa
     4741 cagacggctc ttgaacacaa cattccggtg cagcgagaag tggctcccgg cgtgataaca
     4801 gaaaccggtt atatccaggt tgagcaggat ggtattccct gcgccagtct ctctattcct
     4861 tgtcgctata cccattctcc ggcggaggtt gccagcctgc gtgatttgac tgattgcatc
     4921 cgcctattga ccgctctggc aggtatgtca gcagcacatt tccccgttga gcctgattca
     4981 ggcactacac aagaggcaca tccattatga aaaagatcct tgtggcatgc ggtaccggca
     5041 tgtcgacttc aaccatgatt gcacacaagc tgcaagagtt cctgactgaa cagggcattt
     5101 cagccactac cgcccaatgc tgtctgaatg agatccctct taactgtaac ggtatggacc
     5161 ttattgtaac ctccatgcga accaacagtg attacggcat ccctacgctt aatggcgccg
     5221 ccctgctcac agggatcaac gacgacgcgt taaaacaaca aatcaaggct ctgttaactc
     5281 aataaaagga ggcattatgt ttgattacat cctgtctctc ggcggtaccg ttttcgtccc
     5341 tatcatcatg atcgtgattg gccttatctt ccgcatcccc tggctgcagg cgattaaggc
     5401 aggggtaacg gttgggattg gttttgtcgg tatgggactg gtgatcgtca tggcgatcga
     5461 cagcctcagt ccccctatca aagtgatgat tgagcgtttc ggcctggccc tgcatgtctt
     5521 tgacgttggc gcaggccctg cctccggcgt tggttatgcg acagccattg gcgccatgat
     5581 tatcccagtt atttttcttc tcaatgtcgc catgctggtc acccgtctga ccaaaacgat
     5641 gaacgtcgat atttacaatt actggcacta cgccattacg ggtacggtgg tccagcttat
     5701 gaccggtagc ctgatttatg gtgtgctggg ggcaatctgc catgcggcgc tatcgttaaa
     5761 aatggccgac tggacggcaa aacgggtaca gaacatcgtc gggctggaag gtatttcgat
     5821 accgcaaggc tacggttcca gttcagtacc gcttttcgtt ctacttgatg ctatctatga
     5881 aaaaatccct ttcatgaagg gacgcaatat cgatgctcag gagatccaga aacgctacgg
     5941 catggtcggc gatccggtga ttatcggcgt agtgctcggt ctgatttttg gcctggcggc
     6001 tggtgaaggc tttaaaggtt gcgccagtct gatgattacc gttgcggcaa tcatggtgct
     6061 gttcccgcgc atgatccgcc tgattgttga gggcctgatg cccatctccg acggtgcaag
     6121 aaagttcttc cagaaatatt tcaaaggacg tgaagtgtat atcggtctgg atactgctgt
     6181 gacgctgggg catccaacca ccattgctgt cggtctgcta ctgataccca ttatgttaat
     6241 tctggccagc attttaccgg gcaacaaagt gttacccctc gcagacctgc cggtagcacc
     6301 gttctttatc tgtatggcta cggttatcca ccgcggagac ctggtcagga cgttgataag
     6361 cggggtcatc gtgatgatta ccgtcctgct gatcgccacc cagttcgcgc cttacttcac
     6421 tgaaatggcg ctcaaaggcg gtttcagttt tgccggtgaa agtgcgcaaa tctcagcgtt
     6481 gtccgtgggc aatatgttcg gttggtcgat ttctgaattg atgtcgcttg ggattattgg
     6541 cgttgtcgtg gcggtcggta ttgtcgccag cgtcgtgctg ttcttacgta aacgtgaatt
     6601 atcggaataa ggagtacttt caatgagttg gctgaaagag gttattggga cggaaaaagc
     6661 ggtcatcgcc atgtgccatc tacgtgcatt gcccggcgac ccgagttttg atgcgcagtt
     6721 ggggatgaac tgggttatcg acaaagcctg ggacgatctg atggcgctcc aaaatggtgg
     6781 ggtggatgca gtcatgttct ccaacgagtt tagtcttccc tatctcacga aggtgcgtcc
     6841 ggaaaccact gcggcaatgg cacgaatcat cgggcaatta atgagcgata tccgcatccc
     6901 gtttggcgtg aatgttctgt gggatccggt cgcctcattc gacctggcga tggctaccgg
     6961 cgctaagttt atccgcgaga tatttaccgg tgcctatgcc agcgactttg gcgtctggga
     7021 cactaacgtc ggtgagacca tccgtcatca acaccggatt ggtgcaggcg aagtgaaaac
     7081 cttgttcaat atcgtcccag aggccgccgt gtatctgggc aatcgcgata tctgttctat
     7141 tgctaaatcg acagtattta acaaccatcc tgatgcgctc tgtgtttctg gcctgaccgc
     7201 cgggacgcgt acagacagcg cgctgttaaa acgggtcaaa gaaacggtgc ctgacaccgt
     7261 cgtgttggcc aacacgggcg tctgcctgga aaacgtggag gaacaactca gtatcgccga
     7321 tggctgtgtg accgcaacca cctttaaaaa ggacggcgtt tttgccaatt tcgttgacca
     7381 ggcgcgagtc agtcagttta tggagaaagt gcatcatata cgccgataag atagggataa
     7441 gcagagcgct tatccccggc aaagagcaat aacccttctg cgatccctgc cgccttcgtc
     7501 aaaagcatcg acggggatcg ctaccagatt aacccgaatc ggcatggagt caacgacgga
     7561 tgattaacga tattaaatgg gtacaggcac aacgtgaagc caccgactgg cgtcaggccg
     7621 tggaaatcgc cacccgcccg ctggtagcct atggtgccgc gcagccatgc tacgttaatg
     7681 ggatcatcga aaataccctt aactgggggc cttattatct gatagcccca ggaatcgcct
     7741 tgcctcacgc ccgaccagag caaggcgcaa actataatca ggtcagtatc accacgctac
     7801 gcaccccggt cgcctttggt aacgaggagt gcgaccccgt ctggctgctt ctctgcgtca
     7861 gcgccacaga tgccaacgca catattttga ccattcagcg catcagccag tttattgatt
     7921 cgccacaacg cttagcggca gtgggaaatg ccagcacaga cgatgcactg tttgcgctgg
     7981 tatcaggtta acggaggcgc ttatgatcct gcacccttct ctcgccagcg caaatcctct
     8041 gcactatggt cgtgagctga cggcattgga caatctggat tttgggagcc tgcatctgga
     8101 tattgaggac agcagcttca ttaacaacat tacgtttggg atgaaaacgg tgcaggccgt
     8161 cgccagacag acgcctcatc ccttgtcctt tcatttcatg gttgcccggc cacagcgctg
     8221 gtttaacgcg ctggctgaaa tcagacccgc gtggatcttc gttcatgcag aaacgctgga
     8281 ctatccttcg gaaacgttaa ctgagatccg ccataccggc gcacgagccg gactggtatt
     8341 taatccggca accccgatcg acgcctggcg atatctggcg tctgagctgg acggagtgat
     8401 ggtgatgacc agtgagcccg atgggcaggg acagcggttt attccctcca tgtgcgagaa
     8461 aatccaaaaa gtacgcacgg cttttcctca gaccgagtgc tgggccgacg gtggaattac
     8521 gctggcagct gcacagcaac tggcagcagc cggggcgcaa catatggtga ttgggcgcgc
     8581 cctgttctct tcatctgact atcgcgcgac cttagctcaa ttcgccaccc tgtaagagga
     8641 gattgtttct gatgagccaa cagagacctg accgtatcaa acagatgctg cactacctgt
     8701 ggcaacatcg tcacctctcc acacagcagg ccatggagct ttttggctat gcggaagcga
     8761 ctgtccgtcg tgatttccag tatattgcca accagtatcc aggcatgatc cgcggtcacg
     8821 gttgcctgga cttcgacgac agtacagacg acaaagagta cgtcttcgac gtgaaacgta
     8881 cattgcaatc tgtggcgaaa agagaaatag ctgcccttgc ccgaacaatg atcaaagatg
     8941 gcgactgctt ttttctcgac tccggctcaa cctgcctcga actggcgaaa tgcctggccg
     9001 atgcgcgggt aaaggtcatc tgcaacgaca ttaaaatcgc caatgaactg ggctgttttc
     9061 cccatgtgga aagttacatt atcggaggac tgattcgccc aggttatttt tcggtaggag
     9121 agagtctggc gctggagatg atcaatgcct tttccgtaga acgtgcgttt atctcctgcg
     9181 atgcgctgtc gctggagacg gggatcacca atgccaccat gtttgaggtc ggggtgaaaa
     9241 cacggattat tcaacgctcg cgtgaagtta ttctcatggc cgatcactca aaatttgatg
     9301 ccgttgagcc gcatgctgta gcaacactat catgtattaa aacgatcatc agtgacagcg
     9361 ggttacctga gactattgca cagcgttatc agcgagccgg atgtcagttg tttttacctc
     9421 attcaataaa gtgataagta agttcgttcg agagggattt caagcaaaaa taatcaatgg
     9481 cacccaatag aaaatattgg cgatgcgctc gaacgaataa agaagcccta tgcgcaatcc
     9541 acacgctacg atgttgcaac aacacgccat ctacttttta ttctcattca ctaaatgtgg
     9601 ttgttctgga ttcattattc aaagtgtgta caagatcaca tttaatcact tcattacagt
     9661 tcagcatgct gaacaaagca tattttccac tatgtaatgc cgataccatt tattccatga
     9721 gcaagaagga gccattatgg ttcgcaaagg gtgtaattca ttagtccgtg ctgaaaaaat
     9781 attaacccac attgcatggg ttggtatggc aagttatatg gagttattaa ataaattcca
     9841 gtaccccaaa agcagcctgc ttaatttatt gaatgtaatg gtggagtgtg gtttcttaat
     9901 taaaaacaaa aacggctact attcactggg aataaaaaac tacgaattgg gttgtcaggc
     9961 gttacaccgt caaaatatat ttgaggtgac taagcggcca atgcaggagt tgtcgctgaa
    10021 aagtggactg gtttgtcatc ttggggcgat ggagagcatt tcggctatct atctcgacaa
    10081 gatcgaaagt ccggactctg taccgacgag caaaagctgg attggcaaaa aacttgagtt
    10141 gcatattacc gcgttgggta aggcattatt agcctggaag acacgagaag aactggatta
    10201 ttttttagaa gcactcacgt taactccaca tacgcgcaat acatttaccg ataaaaaatt
    10261 gtttctggaa gaattacaaa aaacacgcct tcggggatgg gccatagaca acgaagaatc
    10321 aacctatggg gcggtatgtt taagtatgcc ggtattcaat atgtataaca gagttaacta
    10381 tgcaatctca ctttctggcg atccggtagt ttattcagga aataagatag acagctatct
    10441 ggaattgctc cggaaatgcg ctgagcaaat atcatatggg ctgggataca gaaacgaaaa
    10501 tgagcattta cgaaaaggaa actgaggtaa tgaaaaaatt cagcggcatt attccaccgg
    10561 tatccagcac gtttcatcgt gacggaaccc ttgataaaaa ggcaatgcgc gaagttgccg
    10621 acttcctgat taataaaggg gtcgacgggc tgttttatct gggtaccggt ggtgaattta
    10681 gccaaatgaa tacagcccag cgcatggcgc tcgccgaaga agctgtaacc attgtcgacg
    10741 ggcgagtgcc ggtattgatt ggcgtcggtt ccccttccac tgacgaagcg gtcaaactgg
    10801 cgcagcatgc gcaagcctac ggcgctgatg gtatcgtcgc catcaacccc tactactgga
    10861 aagtcgcacc acgaaatctt gacgactatt accagcagat cgcccgtagc gtcaccctac
    10921 cggtgatcct gtacaacttt ccggatctga cgggtcagga cttaaccccg gaaaccgtga
    10981 cgcgtctggc tctgcaaaac gagaatatcg ttggcatcaa agacaccatc gacagcgttg
    11041 gtcacttgcg tacgatgatc aacacagtta agtcggtacg cccgtcgttt tcggtattct
    11101 gcggttacga tgatcatttg ctgaatacga tgctgctggg cggcgacggt gcgataaccg
    11161 ccagcgctaa ctttgctccg gaactctccg tcggcatcta ccgcgcctgg cgtgaaggcg
    11221 atctggcgac cgctgcgacg ctgaataaaa aactactaca actgcccgct atttacgccc
    11281 tcgaaacacc gtttgtctca ctgatcaaat acagcatgca gtgtgtcggg ctgcctgtag
    11341 agacatattg cttaccaccg attcttgaag catcagaaga agcaaaagat aaagtccacg
    11401 tgctgcttac cgcgcagggc attttaccag tctgaggaga aactcatgtc tgttcgcaat
    11461 atttttgctg acgagagcca cgatatttac accgtcagaa cgcacgccga tggcccggac
    11521 ggcgaactcc cattaaccgc agagatgctt atcaaccgcc cgagcgggga tctgttcggt
    11581 atgaccatga atgccggaat gggttggtct ccggacgagc tggatcggga cggtatttta
    11641 ctgctcagta cactcggtgg cttacgcggc gcagacggta aacccgtggc gctggcgctg
    11701 caccaggggc attacgaact ggacatccag ataaaagcgg cggccgaggt tattaaagcc
    11761 aaccatgccc tgccctatgc cgtgtacgtc tccgatcctt gtgacgggcg tactcagggt
    11821 acaacgggga tgtttgattc gctaccatac cgaaatgacg catcgatggt aatgcgccgc
    11881 cttattcgct ctctgcccga cgcgaaagca gttattggtg tggcgagttg cgataagggg
    11941 cttccggcca ccatgatggc actcgccgcg cagcacaaca tcgcaaccgt gctggtcccc
    12001 ggcggcgcga cgctgcccgc aaaggatgga gaagacaacg gcaaggtgca aaccattggc
    12061 gcacgcttcg ccaatggcga attatctcta caggacgcac gccgtgcggg ctgtaaagcc
    12121 tgtgcctctt ccggcggcgg ctgtcaattt ttgggcactg ccgggacatc tcaggtggtg
    12181 gccgaaggat tggggctggc aatcccacat tcagcccttg ccccttccgg tgagcctgtg
    12241 tggcgggaga tcgccagagc ttccgcgcga gctgcgctga acctgagtca aaaaggcatc
    12301 accacccggg aaattctcac cgataaagcg atagagaatg cgatgacggt ccatgccgcg
    12361 ttcggtggtt caacaaacct gctgttacac atcccggcaa ttgctcacca ggcaggttgc
    12421 catatcccga ccgttgatga ctggatccgc atcaacaagc gcgtgccccg actggtgagc
    12481 gtactgccta atggcccggt ttatcatcca acggtcaatg cctttatggc aggtggtgtg
    12541 ccggaagtca tgttgcatct gcgcagcctc ggattgttgc atgaagacgt tatgacggtt
    12601 accggcagca cgctgaaaga aaacctcgac tggtgggagc actccgaacg gcgtcagcgg
    12661 ttcaagcaac tcctgctcga tcaggaacaa atcaacgctg acgaagtgat catgtctccg
    12721 cagcaagcaa aagcgcgcgg attaacctca actatcacct tcccggtggg taatattgcg
    12781 ccagaaggtt cggtgatcaa atccaccgcc attgacccct cgatgattga tgagcaaggt
    12841 atctattacc ataaaggtgt ggcgaaggtt tatctgtccg agaaaagtgc gatttacgat
    12901 atcaaacatg acaagatcaa ggcgggcgat attctggtca ttattggcgt tggaccttca
    12961 ggtacaggga tggaagaaac ctaccaggtt accagtgccc tgaagcatct gtcatacggt
    13021 aagcatgttt cgttaatcac cgatgcacgt ttctcgggcg tttctactgg cgcgtgcatc
    13081 ggccatgtgg ggccagaagc gctggccgga ggccccatcg gtaaattacg caccggggat
    13141 gtaattgaaa ttaaaattga ttgtcgcgag cttcacggcg aagtcaattt cctcggaacc
    13201 cgtagcgatg aacaattacc ttcacaggag gaggcaactg caatattaaa tgccagaccc
    13261 agccatcagg atttacttcc cgatcctgaa ttgccagatg atacccagct atgggcaatg
    13321 cttcaggccg tgagtggcgg gacatggacc ggttgtattt atgatgtaaa caaaattggc
    13381 gcggctttgc gcgattttat gaataaaaac tgaaagcata aataaaaaca tccagacacc
    13441 tgaattatcc gactctgcat cattcgttat ttgctcagtc ggataatgat gggtcgctac
    13501 cctgttatga gaggacgtta tgccactaat tatcgttgtg gcagggattg ctttactcct
    13561 gcttttaacc ataaaaatta agcttaatac gtttgtttcg ttaattattg tctcgattgc
    13621 tgtcgccatc gccagtggta tggatttgag taaagtcgtc acctcagtcg aatccgggct
    13681 tggcggtacg ctgggccata ttggtttgat atttggcttc ggtgttatgc tcggtcgctt
    13741 gctggccgat gcgggtggtg cgcagcgaat agcgctgacc atgctgaatt atttcgggaa
    13801 aaataaactt gactgggcag tggtctgttc ggcgtttatt gtcggtatcg cacttttttt
    13861 cgaagtcggt ttaattcttc tggtacctat tttattcgct attgcccgtg aagcaaaaat
    13921 atcaccgatg tttatgtgtg taccgatgct ttctggtttg ctggtcgcac acggtttttt
    13981 gccgccgcac cccggcccga ctgttatcgc cagggaatat ggcgctgatg taggattggt
    14041 actgatatac ggtatcattg tcggcattcc aacctttatt ctttgcggcc cggtattgaa
    14101 taaattctgc cagcgcatta ttccggacgc ctttaaaaaa gaggggaata tagcctctct
    14161 cggcgcgacc cgccgattca gcgaaagcga aatgccaggc tttgggatca gttttctcac
    14221 ggcaatgctg ccggtgatcc tgatggcggt tgtcaccatc atccagatga ctcatgccaa
    14281 aagtgccgcg gattctggct tattttacaa cgtgatactg ttcttaggta attcgacgat
    14341 cgccatgctg atttcactgc tgttcgcgat ctataccatg ggcctggggc gcgggaaaac
    14401 aatccctgac ctgatggatt cttgcggaaa agctatcgcg ggtattgccg gtctgctgct
    14461 gattatcggt ggtggtggtg cattcaagca agttctcatc gactctggcg ttggtcagta
    14521 tatttccacc ctggtttcgg gtatggatat taacccgatc ttgatggcct ggggcgtggc
    14581 cgcattcctg cgtatttgtc tgggttcagc caccgtcgcg gcgatctcta ccgccggtct
    14641 ggtcatccca ttgttggcag tacatcccaa taccaacctg gccttgatta cgctggctac
    14701 cggtgccgga tcctgtattt gttctcacgt taacgatgcc agtttctgga tgattaaaga
    14761 cttttttggc ctgaccacca aagaaacgct gttatcctgg acgttgatgt ccacactctt
    14821 atccatcagt gggctgattt ttattctgct cgccagctta gttttataat cgggttcttg
    14881 ccagtgagcc aacctgttgt tcactgtccc ccataattta gtgagtgacg gcaaccgtct
    14941 gcacggtgac gaatcgctgg cgatcaaaaa ggggcgatat tgaaccgtcg attatgcttg
    15001 atgggtgtac tctcagtaat actgtaaagc cggagcatgt ctccgacttt acagtatggg
    15061 ggaacagcag gcgatcgctc aaatggtatg ctggtttatt tcatgtaaaa gaataaaata
    15121 tctgtataaa atcatgccac tggcaatctg catcaactct ccgggtaaac agggttaaga
    15181 tgagcatttc cccggtttaa tacaggtata caaaagtgga tagagatcaa acaaacagca
    15241 gcctgttcaa tgatgatccc gtacttcatg caacatggct gtactatcag gaaggcaaaa
    15301 gccagactga agttgcagct atcatggggg tgtcccgcgt cacggttgtc aaatacctgc
    15361 aaacagcccg ggaaaatgga ctggttcata tcaacctgga tgtgaatgtc tttggctcta
    15421 tcgacgctgc tttgcaaatt cgcgataaat tcaatctcca gagagtgatc atcgtgccgg
    15481 atggcgaaca tgccggaaaa cgtgatgata caaagctgat gcggactcgc ctttcacgtg
    15541 ccggtggcat gtatctgaat caggtgattg agaatggtga tgtgctgggg gttgcctggg
    15601 gaagaacaat ccatcagatg agcaaaacca tgacgccgaa gtcatgtaaa aatgtcaccg
    15661 ttatacagat gctcggttcc atgccatctc agccagacct gacgatcatt gaatcctcat
    15721 cgcaaatcgc ctacaagctt tcaggccgtg tcgcctcgct gcacgtgccc gccgtggtat
    15781 caagcgccag actggccatg gagttacagg cagagcctat tattcgatcg aattttgacg
    15841 ttctgactcg ctgtacgaaa gccttttttg tcgtgggtaa cgcgcttgat gaaaatcccc
    15901 tgattcgggt gggcgtgctg aataagaagg aaatgcagac gtatcgggat ttaggcgcag
    15961 taggtgtcat ttgtggtcgc ttctatgaca aggaagggat gcccgtcgtg gctgatgttg
    16021 accagcgtat tctgggaatc agtctggctc agctaaggca gattgaacgg aagatcttcc
    16081 ttgctggtgg ggaaagaggc tatgacgcaa cgttgggtgc cctgctggga ggatatgtca
    16141 cggacctgat tgttgatgaa ggaacggcag agtttttgct ggcgtgtgaa ttaccacact
    16201 aaaagaatga acaactgccc catgtggata tggggcagga ttttattgat tagcggcgat
    16261 aataagaaca atacccacaa tgtatgaaat aaacatggtc agcagcagtt tattggttcc
    16321 ggctggcgca ctggcaaatt ctctccagat gaagactccc cagatcacgg caatcattgt
    16381 ggccccctgt cccagaccgt aggaaatggc ataccccgcc tgaccagacg caatcagact
    16441 gaatgccaga ccgacacacc aaatcatacc gcccagccag ccgcaaacat gatcccgtaa
    16501 gctcccggaa aaatacattt tcccgttgac cggttctcct gaaataggtt ttttcatgac
    16561 cagcgtgtta aggacaaagt tcgacaagaa cagccccacg gcaaacagaa caagtgctga
    16621 gtaaggcgtc atcagaccgc tggcaggctg gctgaaattg tcagacatgg agtccgccaa
    16681 gaagcggaaa aaccatccca tgatgatacc cgccagaatg gcagtaatga gacctttatt
    16741 acggcgagat ttatccgcct cctgcgtgac acgaccatag gctatcgcgg taaaaataat
    16801 ggcgatcaca acgcaggcga caccgaggaa cagaatgagc ggatcgccct gcggattgcc
    16861 gatataggtg gtgatcacac ccagagccag tgccagcccc acacccacag gaaaagctac
    16921 cgccataccg gcaagattaa tagaagcgac cagaagaata ttggaaatat tgaagataat
    16981 ccctgccagt attgccgaca tcagactact gctgctcgcc tgctgaatat caggtataaa
    17041 actgcgaccg gcttcgccca tagaacccag cgtgaaggca aataacaaag aacacagcaa
    17101 taatccaatt gaataatccc aatagaatag agggaactcc cattttttat tgctgacaag
    17161 tttagttgta ttcgcccatg aaccccagca aatcatggta ataaaacaca ttattatggc
    17221 gacagcatac gattccacga taaacataaa taactccttg tggtagttat aatcaatcgt
    17281 tgtggacaga gggaatagca gccattctgc gaatggctgt catatgttat taattaaaat
    17341 aaagctgtaa ttacgctttc aaaccatacg ttttgaattt ttcaaggacg atggtcattt
    17401 cggcttcaga cagcacggga acatcgtgat gtaatccgcc acatttgatg tataaatcag
    17461 ccagcgtctc ggtttcaccc gccagccaca acgtcttttc cagcgtgacg tccatggcca
    17521 gcataccgtg gtgctgcatc agcaaggact tactttctct gattccttta gatacaccgt
    17581 cggccagttc aggactgcca aaggtataat agggaataca gggaatatga tccgtccccg
    17641 ataccgccac catataatga atggccggaa ttgaatgatt atgtattgcc accgccgtgg
    17701 aattcaccgc atgcgtatgt atgacagcat tcaattctgg tctggcttta taacacgcca
    17761 gatgaaataa ccattcactt gatggtattt tacccgcttc aggtattccc ttatcgtcta
    17821 caaagacaat catgtccggc gtcatctttg aataagcgat cccactcggc gtaatcaaca
    17881 tgccaccggt atacctggca ctcacattac ctgatgttcc ttgattaaga cctgacgcat
    17941 tcatttcccg acaagtggaa atgatttttt ctgctaactt aatccgttcc attcctgatc
    18001 cttaatagca atggccgccg ttttaatgct gttccccctg gagtgcttta ctcttcaggt
    18061 cagcaaacat atccgccagc gctgacttac tggcagcaca gactccccgc tcagtaatga
    18121 agccggtaat gtatcgggca ggcgtgacgt cgaaagcgta gttcccacaa cgggttcctt
    18181 caggggcggt attgacccag ctcaattctc cctgagggtt tatcccatag acatgggact
    18241 gctcttttcc gtcacgttgc tcaatcggaa tgctttttcc gtcctcgata gtccagtcaa
    18301 tagtgggaga aggtaacgcg acgtagaagg gaacatggtt atcatgggcc gccagcgcct
    18361 taagataggt accaatttta ttacagacat cccctctggc cgtggttcga tcggtcccaa
    18421 caatgcagag atcaacgtca ccgtgttgca tcaggtggcc acccgcgttg tcggcgatca
    18481 gggtgtgagg aataccgtgt gagcccagtt caaatgccgt gagtccgccc tgattacgtg
    18541 gccgcgtttc atccacccag acgtgaacag ggattccgtt ttcatgcgct ttgtagatgg
    18601 gagaaagcgc ggttccccag tctacagtcg ccagccagcc tgcgttacaa tgtgtcagga
    18661 tattgaccac actcccggcc ggttttttct gggctatttc gcggatgact tctgtaccat
    18721 gtaatccaat ttgttcacac aacgcgacat cttcctcggc aatttctgcc gccagcgccc
    18781 aggccacatc cttgcgctct gatacacaga gatccttcag tgtgtctatc atcctgtcga
    18841 gggcccattt gagattaatt gccgttggtc gggttttgat aagaaggtca tagtaacgct
    18901 gcagacccat gtcactggca tcgtgattca ttcccagcgc gatgccatag gctgcaacga
    18961 caccaataag cggagcgcca cgaacccaca tctcctgaat agccgtagcg gccatttccg
    19021 cagaggtaag cgccaccacc tcgaacttaa aaggcaactt agtctgatcg atgatttcta
    19081 ccgcttttcc atccccggaa acccagaccg tacgatagtg tttaccttta atattcataa
    19141 tgacctctta cttaaccttc gcagactgga tgacggaatc catattttta aactggcggg
    19201 cattgacgat aagatcgcgc gccatggtca atgcacgtcg ttcacatcct gcgcgaagct
    19261 ctgtattttc aatctgtttc agctcggcaa cgccagcaaa accaatgatc cggcgattca
    19321 tttccatgcc ggcattcacc agggaatctt caagcagcgt ggcaaaaaac tgatcctgtg
    19381 cgtcctcaag agcggccctg ttctgttgat acatttctgt cggccacgcg tcgccctgtg
    19441 ttttgttgtc ccagagctgg cggaactccc gggtgaaaac ggaccaggtt tggacaatct
    19501 gctgaagcag ccattcctga tagtcagcgc gacgttgctc attcgcatcc cacccagggc
    19561 gtgagaagta cgccagcagg aggttgccaa tatagttgcc gatatcaaac gccattggcc
    19621 ccatgaaacc gaactccgga tcaatcactt tggtatccgt gtcggtcacc atgattgagc
    19681 ctgaatgaag gtcgccatgt aacaatgcct gcgcttctgt cataaatttg tacttataac
    19741 gcatggcaac ctggatcatc tctacatcag cccaggcctt atggacggca tcgtccagct
    19801 caggagaagt ccagttattc cgttcagcgt tgtaataggg ctctgtgaag atcagatctt
    19861 ccgtaatttt gcacagctca tgattcaacg cgaactcggc agtaagcgct tttttctgtt
    19921 ctgctgacat gccaatgtca gacgtattga agagagtctg tgctaaaaat ctgccgatat
    19981 cttcagctag tttagggaat tttttaccgt taatcagttc cttacgcaga atcacgtgag
    20041 gagtcagata ctccatggca aacagcgcca tttccgggtc gtaaaaatag acctgcggaa
    20101 tcagtgtgtg acccgcgtac ttggcttcct gacataagac gttgtactca aagcccgcac
    20161 ggctcagaga taaaggccat ccttcgcccc ctgcacgaag ccagggcaga gcttgtttta
    20221 caatgatggt tttatgtgtc ccctcaacga taaacaccat gttcaggttt ccgtccccca
    20281 cttctgaaac tttccactct tcaggtaacc ctcccggttc gcatgaaggg gtcagtctgg
    20341 acgagagata acccggcagc gtgtcacatg tcagcggctt gtaacctgat ggaattgaat
    20401 ccgtcataac agttctcctt gtctacacag aatgataatc aacttgtcat gtgacaacta
    20461 tacattatca aatgtaaaat tatttgtgac acatgtagat tttttgaact gtataatatt
    20521 aaaaatgatg tcgtatggag tcatgatctt tacaggaatc agcagaggaa aacatctatt
    20581 attaccatgt aattcatgtt gttatgtcgc aacaatagta ctgcacgggg aattattacg
    20641 ggcaaactta ggcgaaagga agtactgaag tgacagaata aatcacactt tttgtacata
    20701 tgtacttttc tattgtcttt tgttgtggaa acaaatagtg tttgtcatgc cgatgctaat
    20761 agacagatag attcgagggc caacagcccc atcatcatga tggagcaaca gaggtgagtc
    20821 taacgtttcc gcacttctta tcgatcggca ctaacgatca atatcgataa actgatctat
    20881 caaaacgatt atctttcttg caacctgcct ggttatcctt ctcttacatt gctcgcagaa
    20941 aagccctttt attacggtta gttatctagc tgagacttgc tacgcccatt ttccatgtac
    21001 cccaaatcac gaggtcacaa aggcgcggaa agatgaagag atgtgcacgt tgcaacaaaa
    21061 aaagaagcgt ccttgaacag aggctctcac tcgataaaga attctgcgac gagtgcttat
    21121 tcgatatcga tgaggaaatc agggaaagaa ggattatcac gatcagacgc ctcaaagatg
    21181 acaacgaaag aaaatcgtag ctcaatatga aaaatggaat cgatgtcagc gtcattatcc
    21241 ctgcttacaa cgccgctgaa accagcgtta gtttggttac taaaatcctc agtgaacccc
    21301 acttggctat tgaacttatc attgtcgatg acggttctac tgatgaaact ggcaacgttc
    21361 ttcgtcaaat ccacgatgat cggctcattc tcattgagca ggccaatcaa ggtgtctatg
    21421 ccgcccgaaa tgctgctcta gccttgcacc aaggagaata ggttatattc cttgatgcgg
    21481 acgacgatat agcagatggg ttcatagaaa aaagatggca ggcagttagt acgtcgcaag
    21541 ccgacgtcat gatctttaat ggctggcatt cctgtgctga ccaacatcga agagcggttc
    21601 acaaaaagca gccctatggt acgcctctgt caggttatta atggattaga cactgtgtta
    21661 cacaccgcga atggccgcat tatctgtggc tgcagatggt caggccatcc tatgtcaggc
    21721 agcattcact gcgcttccag tccggcagga gccataaaga cattttttaa acggttcatc
    21781 ttgcagcgag caatggttga ttctgtacat tggatataaa ggactatatc tatatcaaca
    21841 ataaggcctc tatcacacat cggccggatt actacgttgt tcgtgctata aactatgttg
    21901 aagtgatcgc gaaaatcatc caagtagcgg aacaagaaca aaacaaaaaa taaaggtatt
    21961 tttgtaccgg catgcgctgg tagaggcacg tcattttttg ggactctacc gcaatcgcgt
    22021 tggggataaa tcgggcatga aggactgctt caaagcgaac atattcgcag cccatttatt
    22081 cagaggtatc agcagtttta gcgatgtgtt tttctttatt aagctgctgg gcaagctatt
    22141 ctgataagtt gtgcgttgct acacgacacc agcgcacagc ttaatttcac gctttctgac
    22201 taaattgttc aggccttttc tataggtgca aacacaactc cccgtacttt cctcttccaa
    22261 ctcaaaatca tcgcaccttg ctgacccaag aagggagcat accctgcact agtatccgca
    22321 cagctaatac tctattgata ccaacaacgc ttcagcacaa caaagtaact cgataatcac
    22381 aactcaaagt gaagatgcca aaaactcgtt tgagagcaat aaattcaata acatgaaaaa
    22441 aatgtatttt gcattgaaaa tcttacttat tgcgcttggc tacaattgca tgcttggtaa
    22501 tatagattat aaaacaccag actagctgcc tggtaagtaa tgtaatagca gaggaaatat
    22561 cacgatggat cttgtagata gtgtcgaagc aggtaagctg acgatcagtg aattgattga
    22621 tgccctggcg aaagataaaa attactcagc ttccaagtgg gatcagcgat accgtgaatt
    22681 tacgaccttg ctacaacaaa cctcaacttt ttctgaacct gaaacggatg gtttagtcaa
    22741 gaggctctgg tatgaacgcg ataacggtat tgccagtatt cgccagggcg ttccatcctt
    22801 agcagaatat cagcaaagcc tgccactgct tagagaacta actgaacgaa ttcggcaaca
    22861 gccggatgaa gaaacttacc aatatgttgg caatgcacta caacaagcta aagaaaacgg
    22921 acttctcaag cgtatgtata ggagtttgag aaatcgcgtc tttgccgcgt tctcgccaga
    22981 aaactacacc agtactgtgg atgagaatgc ttttagtaaa gcagcagaat tcctaaatca
    23041 gcacttccat ctcggtctgg cactgaccgg aaattggtta cagaaaaact atgaattgaa
    23101 acaagccata cacgcccaat ctcctgacac agatccttat tacgtgaata tggccatctg
    23161 gcatctctat gaattgctcc gtgaacgcga taatgaacaa aagcaggaga aaatagcgag
    23221 cactacattc ataaccagca atgagcccat cgagaacaag atcatcctac attcaccaac
    23281 taacgtgatc ttctttggcc cccctggcac tggcaaaacc ttcaggttgc agcaaaaaat
    23341 gaaagagtac acttctcatg ctgttccagc tgatcgtgat gcctggctgg attctcgcct
    23401 tgaatcgttg aattggatgc aggttataac gctggtgctg ctcgatcttg ggaaacgagc
    23461 caaagttcgc caaattattg aacatatgtg gtttcaacgt aaggcattat taaacggtcg
    23521 taatggcaac ctatcgaata cagcctgggc agctttgcaa tcctatacag ttcccgagtc
    23581 gttaaccgtt gattataaga atcggcgtga gcctgccgta tttaacaaaa cagataacag
    23641 cgaatggttg ctagttgatt cacagctcga gcaagtggag gatttggtag agctctacgc
    23701 cgaacttaaa cgaggcccta aatctgccga agccatccag cgttttgcgg tggttacgtt
    23761 ccaccaatct tacggctatg aagaatttat tgaaggtata cgcgctcgct ctgacgagag
    23821 tggcaatatc tcttatccca ttgagccggg tatctttatg cgcctttccc aacgtgccaa
    23881 tgccgatcca ggacatcgct acgccatttt cattgatgag atcaatcgcg gtaacatatc
    23941 caagatcttt ggtgaactaa tctcactcat tgaagtagac aagcgtgcag gcatgcccaa
    24001 tgcaatgagc ctacaactgg cttatagcgg tgatcacttc agcgtacccg ccaatgtcga
    24061 tatcatcgga gccatgaata cagcggaccg ttctttagct ctgatggaca cggctttgcg
    24121 ccgtcgcttt gactttgtcg aaatgatgcc cgatctctct ttactgagtg gagctaaggt
    24181 gaaaggcata gagctcgagt cgttgttaga gaaactcaat agccgcatcg aggctcttta
    24241 cgatcgtgaa catacgctgg ggcatgcgtt ctttatgccg gtaaaaaatg cactcgatgc
    24301 cggtgatgaa gaagctgcgt ttaaacaatt gaagatcgca ttccagaaaa agatcattcc
    24361 gcttttacag gaatactttt tcgatgactg gaacaagatc cggttggtgc tggcagacaa
    24421 tcaaaagcaa gacgacaacc tgcaattcgt gattgagaaa accgacgatc tcgatacgct
    24481 ttttggtaac aaccatggtt tacgacacca tgatcagcaa tcaacagctt acgaactcaa
    24541 agatttcgat caagagatct ggaatattcc acaggcttat cgttcaattt atcagcccca
    24601 gcagactccc cttgatgagc aggctgtaaa tcatgggtga agttatctcc gtttttgaat
    24661 atgacctatt ggggagtgac aaagcggctt cagttggcgc aaaactggtg cctccactgg
    24721 tctttaatta tttagaagcg cttagcttgg caagtaatca agggagccag ttcctcaaac
    24781 tcacctctcg ctcaggcttc aaattgcttc aggttcaaaa ctacgcgggg atgctttcaa
    24841 cacctcacgg tttccagctt gagatactgc ccaaggttgg taaaaacctc acagcggcta
    24901 atgcacgtca aacattactg acgatgctaa gccatttgcc tgggtttcga catattgaaa
    24961 ctcagcaagc cactcttcag gctcagcgta tgcctttgct tgagattttt attcaccagt
    25021 ttttgcatag cgttagccaa ttacttaaac aaggtttacg ctccgactat gtgagcgagc
    25081 aaggtaatct ggcttttatg aagggcaagc tgatgctttc tgcacagctg cgacataacg
    25141 cggtgaatcg ccataagttt tgtgtcgatt atgatgacta catgcctgat tgtgcagcca
    25201 atcggctctt acactccgcg ctagataagc ttcttagtct aaagctgtca tcagagaatc
    25261 aacgctggct ttacgaactg cgctttgcgt ttgacggtat tccacttagc cgggatattg
    25321 aacgtgatat aaacaactta cgcctcgagc gtggtatggc tcattacaac gagccaatgg
    25381 cgtgggcgca attgatcctg agaggaatga gtccgagtgc tttgcaggga aacaccaaag
    25441 cgatatcact tttgttccct atggaagcgg tatttgaatc ctttgtggca cagaccctac
    25501 cctacgaatt accttctcat ctcaaagtaa taccacaagc ggctacttat tctcttgtga
    25561 agcacgggtc taacgattgc tttaaacttc ggccggattt gttgattcag tcacgtcgac
    25621 cggtacaaac caaattggtg atggacacaa aatggaagct aataaatagc aactcccaaa
    25681 aaacaacaca gtatgggcta gcgcaagccg acttctatca aatgtttgcc tacggtcaaa
    25741 aatacctaaa tgggattggc gaaatgtact taatctatcc tgcgcatgat caatttaacc
    25801 aaccgataca gcagcatttt gctttctcta aaacattaaa actatgggtt gttccgtacc
    25861 agataacagc aaaccgcggg gagaggatga tgtgggtgaa ggatgtgtcc agtatataaa
    25921 tttctagcca agctggcgat gaaccag
//
LOCUS       DAHHTG010000065        25214 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.065, whole genome shotgun sequence.
ACCESSION   DAHHTG010000065 DAHHTG010000000
VERSION     DAHHTG010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 25214)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25214)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25214
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.065"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            314..1612
                     /gene="kgtP"
                     /locus_tag="NBI74_002595"
     CDS             314..1612
                     /gene="kgtP"
                     /locus_tag="NBI74_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate permease"
                     /protein_id="HCD1932911.1"
                     /translation="MAESTVTADSKLTSSDTRRRIWAIVGASSGNLVEWFDFYVYSFC
                     SLYFAHIFFPSGNTTTQLLQTAGVFAAGFLMRPIGGWLFGRIADKHGRKKSMLLSVCM
                     MCFGSLVIACLPGYETIGTWAPALLLLARLFQGLSVGGEYGTSATYMSEVAVEGRKGF
                     YASFQYVTLIGGQLLALLVVVVLQHTMEDAVLREWGWRIPFALGAVLAVVALRLRRQL
                     DETSQQETRALKEAGSLKGLWRNRRAFIMVLGFTAAGSLCFYTFTTYMQKYLVNTAGM
                     HANVASGIMTAALFVFMLIQPIIGALSDKIGRRTSMLCFGSLATIFTVPILSALQNVS
                     SPYAAFGLVMCALLIVSFYTSISGILKAEMFPAQVRALGVGLSYAFANAIFGGSAEYV
                     ALSLKSIGMETAFFWYVTLMAVVAFLVSLTLHRKGKGMRL"
     gene            complement(1609..1932)
                     /locus_tag="NBI74_002596"
     CDS             complement(1609..1932)
                     /locus_tag="NBI74_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiM family lipoprotein"
                     /protein_id="HCD1932912.1"
                     /translation="MRILFVCSLLLLSGCSHMANDNWSGQDKAQHFIASAMLSAAGNE
                     YSQHQGMSRDRSAMFGLMFSVSLGASKELWDSRPEGSGWSWKDFAWDIAGASTGYTVW
                     QLTRH"
     gene            complement(1978..3333)
                     /gene="pssA"
                     /locus_tag="NBI74_002597"
     CDS             complement(1978..3333)
                     /gene="pssA"
                     /locus_tag="NBI74_002597"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HCD1932913.1"
                     /translation="MLSKFKRNKHQQHLAQLPKISQSVDDVDFFYAPADFRETLLEKI
                     ASAKQRICIVALYLEQDDGGKGILNALYEAKRQRPELDVRVLVDWHRAQRGRIGAAAS
                     NTNADWYCRMAQENPGVNVPVYGVPINTREALGVLHFKGFIIDDSVLYSGASLNDVYL
                     HQHDKYRYDRYHLIRNRKMSDIMFEWVTQNIMNGRGVNRLDDVNRPKSPEIKNDIRLF
                     RQELRDAAYHFQGDADNDQLSVTPLVGLGKSSLLNKTIFHLMPCAEQKLTICTPYFNL
                     PAILVRNIIQLLREGKKVEIIVGDKTANDFYIPEDEPFKIIGALPYLYEINLRRFLSR
                     LQYYVNTDQLVVRLWKDDDNTYHLKGMWVDDKWILITGNNLNPRAWRLDLENAILIHD
                     PQLELAPQREKELDLIREHTTIVKHYRDLQSIADYPVKVRKLIRRLRRIRIDRLISRI
                     L"
     gene            complement(3447..6107)
                     /gene="pat"
                     /locus_tag="NBI74_002598"
     CDS             complement(3447..6107)
                     /gene="pat"
                     /locus_tag="NBI74_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein lysine acetyltransferase"
                     /protein_id="HCD1932914.1"
                     /translation="MSQRGLEALLRPKSIAVIGASMKPNRAGYLMMRNLLAGGFNGPV
                     LPVTPAWKAVLGVLAWPDIANLPFTPDLAVLCTNASRNLALLEELGEKGCKTCIILSA
                     PASQHEDLRACALRHNMRLLGPNSLGLLAPWQGLNASFSPVPIKRGKLAFISQSAAVS
                     NTILDWAQQREMGFSYFIALGDSLDIDVDELLDYLARDSKTSAILLYLEQLSDARRFV
                     SAARSASRNKPILVIKSGRSPAAQRLLNTTAGMDPAWDAAIQRAGLLRVQDTHELFSA
                     VETLSHMRPLRGDRLMIISNGAAPAALALDALWSRNGKLATLSEETCQKLRDALPGHV
                     AISNPLDLRDDASSEHYVKTLDILLHSQDFDALMVIHSPSAAAPATESAQVLIEAVKH
                     HPRSKYVSLLTNWCGEHSSQEARRLFSEAGLPTYRTPEGTITAFMHMVEYRRNQKQLR
                     ETPALPSNLTSNTAEAHLLLQQAIAEGATSLDTHEVQPILQAYGMNTLPTWIASDSTE
                     AVHIAEQIGYPVALKLRSPDIPHKSEVQGVMLYLRTANEVQQAANAIFDRVKMAWPQA
                     RVHGLLVQSMANRAGAQELRVVVEHDPVFGPLIMLGEGGVEWRPEDQAVVALPPLNMN
                     LARYLVIQGIKSKKIRARSALRPLDVAGLSQLLVQVSNLIVDCPEIQRLDIHPLLASG
                     SEFTALDVTLDIAPFEGDNESRLAVRPYPHQLEEWVELKNGERCLFRPILPEDEPQLQ
                     QFISRVTKEDLYYRYFSEINEFTHEDLANMTQIDYDREMAFVAVRRIDQTEEILGVTR
                     AISDPDNIDAEFAVLVRSDLKGLGLGRRLMEKLITYTRDHGLQRLNGITMPNNRGMVA
                     LARKLGFNVDIQLEEGIVGLTLSLAQREES"
     gene            complement(6139..6837)
                     /gene="tapT"
                     /locus_tag="NBI74_002599"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
     CDS             complement(6139..6837)
                     /gene="tapT"
                     /locus_tag="NBI74_002599"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
                     /EC_number="2.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-uridine aminocarboxypropyltransferase"
                     /protein_id="HCD1932915.1"
                     /translation="MTENAVLQLRAERIARATRPFLARGNRVRRCQRCLLPEKLCLCS
                     TITPAQAKSHFCLLMFDTEPMKPSNTGRLIADILPDTVAFQWSRTEPSQDLLDLVQNP
                     DYQPMVVFPASYADEQREVIFTPPADKPPLFIMLDGTWPEARKMFRKSPYLDNLPVIS
                     VDLSRLSAYRLREAQAEGQYCTAEVAIALLDMAGDTRAATGLGEHFTRFKTRYLAGKT
                     QHPGSITAEQLESV"
     gene            complement(6906..7325)
                     /gene="trxC"
                     /locus_tag="NBI74_002600"
     CDS             complement(6906..7325)
                     /gene="trxC"
                     /locus_tag="NBI74_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxC"
                     /protein_id="HCD1932916.1"
                     /translation="MNTVCTHCQAINRIPDDRIEDAAKCGRCGHDLFDGEVINATGET
                     LDKLLKDDLPVVIDFWAPWCGPCRNFAPIFEDVAQERSGKVRFVKVNTEAERELSSRF
                     GIRSIPTIMIFKNGQVVDMLNGAVPKAPFDSWLNESL"
     gene            7532..8569
                     /locus_tag="NBI74_002601"
     CDS             7532..8569
                     /locus_tag="NBI74_002601"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HCD1932917.1"
                     /translation="MNDEMKGKSGKVKVMYVRSDDDSDKRTHNPRTGKGGGRPGKSRA
                     DGGRRPARDDKQSQHRDRKWEDSPWRTVSRAPGDETPEKADHGGISGKSFIDPEVLRR
                     QRAEETRVYGENACQALFQSRPEAIVRAWFIQSVTPRFKEALRWMAANRKAYHVVDEA
                     ELTKASGTEHHGGVCFLIKKRNGTTVQQWVSQAGAQDCVLALENESNPHNLGGMMRSC
                     AHFGVKGVVVQDAALLESGAAIRTAEGGAEHVQPITGDNIVNVLDDFRQAGYTVVTTS
                     SEQGKPLFKTSLPAKMVLVLGQEYEGLPDAARDPNDLRVKIDGTGNVAGLNISVATGV
                     LLGEWWRQNKA"
     gene            complement(8617..9306)
                     /gene="ung"
                     /locus_tag="NBI74_002602"
     CDS             complement(8617..9306)
                     /gene="ung"
                     /locus_tag="NBI74_002602"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HCD1932918.1"
                     /translation="MANELTWHDVLAEEKQQPYFLNTLQTVASERQSGVTIYPPQKDV
                     FNAFRFTELGDVKVVILGQDPYHGPGQAHGLAFSVRPGIATPPSLLNMYKELENTIPG
                     FTRPNHGYLESWARQGVLLLNTVLTVRAGQAHSHASLGWETFTDKVISLINQHREGVV
                     FLLWGSHAQKKGAIIDKQRHHVLKAPHPSPLSAHRGFFGCNHFVLANQWLEQRGETPI
                     DWMPVLPAESE"
     gene            9611..9994
                     /gene="grcA"
                     /locus_tag="NBI74_002603"
     CDS             9611..9994
                     /gene="grcA"
                     /locus_tag="NBI74_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007728348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autonomous glycyl radical cofactor GrcA"
                     /protein_id="HCD1932919.1"
                     /translation="MITGIQITKAANDDLLNSFWLLDSEKGEARCIVAKAGFAEDEVV
                     AVSKLGDIEYREVPVEVKPEVRVEGGQHLNVNVLRRETLEDAVKHPEKYPQLTIRVSG
                     YAVRFNSLTPEQQRDVIARTFTESL"
     gene            complement(10050..10637)
                     /gene="eamB"
                     /locus_tag="NBI74_002604"
     CDS             complement(10050..10637)
                     /gene="eamB"
                     /locus_tag="NBI74_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/O-acetylserine transporter"
                     /protein_id="HCD1932920.1"
                     /translation="MTPTLLSAFWTYTLITAMTPGPNNILALSSATSHGFRQSTRVLA
                     GMSLGFLIVMLLCAGISFSLAVIDPAAVHLLSWAGAAYIVWLAWKIATSPTKEDGLQA
                     KPISFWASFALQFVNVKIILYGVTALSTFVLPQTQALSWVVGVSVLLAMIGTFGNVCW
                     ALAGHLFQRLFRQYGRQLNIVLALLLVYCAVRIFY"
     gene            10740..11621
                     /locus_tag="NBI74_002605"
     CDS             10740..11621
                     /locus_tag="NBI74_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCD1932921.1"
                     /translation="MDLRRFITLKTVVEEGSFLRASQKLCCTQSTVTFHIQQLEQEFS
                     VQLFEKIGRRMCLTREGKKLLPHIYELTRVMDTLREAAKKESDPDGELRVISGETLLS
                     YRMPQVLQRFRQRAPKVRLSLQSLNCYVIRDALLNDEADVGVFYRVGNDDALNRRELG
                     EQPLVLVASPQIADVDFTEPGRHNACSFIINEPQCVFRQIFESTLRQRRITVENTIEL
                     ISIESIKRCVAANIGVSYLPRFAVVKELECGELIELPFGEQSQTITAMCAHHAGKAVS
                     PAMHTFIQCVEECFLPG"
     gene            complement(11654..12988)
                     /gene="srmB"
                     /locus_tag="NBI74_002606"
     CDS             complement(11654..12988)
                     /gene="srmB"
                     /locus_tag="NBI74_002606"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase SrmB"
                     /protein_id="HCD1932922.1"
                     /translation="MTVTTFSELELDESLLEALQDKGFTRPTAIQAAAIPPALDGRDV
                     LGSAPTGTGKTAAYLLPALQHLLDFPRKKSGPPRILILTPTRELAMQVADHARELAKH
                     THLDIATITGGVAYMNHAEVFSENQDIVVATTGRLLQYIKEENFDCRAVETLILDEAD
                     RMLDMGFAQDIEHIAGETRWRKQTLLFSATLEGDAIQDFAERLLEDPVEVSANPSTRE
                     RKKIHQWYYRADDLEHKTALLVHLLKQPEATRSIVFVRKRERVHELANWLREAGINNC
                     YLEGEMVQGKRNEAIKRLTEGRVNVLVATDVAARGIDIPDVSHVFNFDMPRSGDTYLH
                     RIGRTARAGRKGTAISLVEAHDHLLLGKVGRYIEEPIKARVIDELRPKTRAPSEKQTG
                     KPSKKVLAKRAEKKKAKEKEKPRVKKRHRDTKNIGKRRKPSGTGVPPQTTEE"
     gene            13120..13857
                     /gene="trmN"
                     /locus_tag="NBI74_002607"
     CDS             13120..13857
                     /gene="trmN"
                     /locus_tag="NBI74_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(1)(Val)
                     (adenine(37)-N(6))-methyltransferase TrmN"
                     /protein_id="HCD1932923.1"
                     /translation="MSQSTSVFRRNGFTFKQFFVAHDRCAMKVGTDGILLGAWAPVAG
                     VKRCLDIGAGSGLLALMLAQRTDDSVMIDAVELESEAAAQAQENINQSPWAERINVHT
                     ADILQWITQQTVRFDLIISNPPYYQQGVECATPQREQARYTTTLDHPSLLTCAAECIT
                     EEGFFCVVLPEQIGNGFTELALSMGWHLRLRTDVAENEARLPHRVLLAFSPQAGECFS
                     DRLVIRGPDQNYSEAYTALTQAFYLFM"
     gene            complement(13842..15464)
                     /gene="nadB"
                     /locus_tag="NBI74_002608"
     CDS             complement(13842..15464)
                     /gene="nadB"
                     /locus_tag="NBI74_002608"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HCD1932924.1"
                     /translation="MNTLPEHSCDVLIIGSGAAGLSLALRLADQHQVIVLSKGPVTEG
                     STFYAQGGIAAVFDETDSIDSHVEDTLIAGAGICDRHAVEFVASNARSCVQWLIDQGV
                     LFDTHIQPNGEESYHLTREGGHSHRRILHAADATGREVETTLVGKAQNHPNIRVLERS
                     NAVDLIVSDKIGLPGTRRVVGAWVWNRNKETVETCHAKAVVLATGGASKVYQYTTNPD
                     ISSGDGIAMAWRAGCRVANLEFNQFHPTALYHPQARNFLLTEALRGEGAYLKRPDGTR
                     FMPDFDERGELAPRDIVARAIDHEMKRLGADCMFLDISHKPADFIRQHFPMIYEKLLG
                     LGIDLTKEPVPIVPAAHYTCGGVMVDDHGRTDVEGLYAIGEVSYTGLHGANRMASNSL
                     LECLVYGWSAAEDISRRIPYAHGVSTLPPWDESRVENPDERVVIQHNWHELRLFMWDY
                     VGIVRTTKRLERALRRITMLQQEIDEYYAHFRVSNNLLELRNLVQVAELIVRCAMMRK
                     ESRGLHFTLDYPELLTHSGPSILSAGNHYINR"
     gene            15720..15875
                     /gene="rseD"
                     /locus_tag="NBI74_002609"
     CDS             15720..15875
                     /gene="rseD"
                     /locus_tag="NBI74_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rpoE leader peptide RseD"
                     /protein_id="HCD1932925.1"
                     /translation="MIRLQHDKQKQMRYGTLQKRDTLTLCLLKLQLMEWRFDSAWKFG
                     LGRLYLG"
     gene            15872..16447
                     /gene="rpoE"
                     /locus_tag="NBI74_002610"
     CDS             15872..16447
                     /gene="rpoE"
                     /locus_tag="NBI74_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005070454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoE"
                     /protein_id="HCD1932926.1"
                     /translation="MSEQLTDQVLVERVQKGDQKAFNLLVVRYQHKVASLVSRYVPSG
                     DVPDVVQEAFIKAYRALDSFRGDSAFYTWLYRIAVNTAKNYLVAQGRRPPSSDVDAIE
                     AENFESGGALKEISNPENLMLSEELRQIVFRTIESLPEDLRMAITLRELDGLSYEEIA
                     AIMDCPVGTVRSRIFRAREAIDNKVQPLIRR"
     gene            16480..17130
                     /gene="rseA"
                     /locus_tag="NBI74_002611"
     CDS             16480..17130
                     /gene="rseA"
                     /locus_tag="NBI74_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-E factor RseA"
                     /protein_id="HCD1932927.1"
                     /translation="MQKEQLSALMDGETLDSELLNELAHNPEMQKTWESYHLIRDSMR
                     GDTPEVLHFDISSRVMAAIEEEPVRQPATLIPEAQPAPHQWQKMPFWQKVRPWAAQLT
                     QMGVAACVSLAVIVGVQHYNGQSETSQQPETPVFNTLPMMGKASPVSLGVPSEATANN
                     GQQQQVQEQRRRINAMLQDYELQRRLHSEQLQFEQAQTQQAAVQVPGIQTLGTQSQ"
     gene            17130..18086
                     /gene="rseB"
                     /locus_tag="NBI74_002612"
     CDS             17130..18086
                     /gene="rseB"
                     /locus_tag="NBI74_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E factor regulatory protein RseB"
                     /protein_id="HCD1932928.1"
                     /translation="MKQLWFAMSLVTGSLLFSANALATPASGALLQQMNLASQSLNYE
                     LSFISINKQGVESLRYRHARLDNRPLAQLLQMDGPRREVVQRGNEISYFEPGLEPFTL
                     NGDYIVDSLPSLIYTDFKRLSPYYDFISVGRTRIADRLCEVIRVVARDGTRYSYIVWM
                     DTESKLPMRVDLLDRDGETLEQFRVIAFNVNQDIGSSMQTLAKANLPPLLSVPVGEKA
                     KFSWTPTWLPQGFSEVSSSRRPLPTMDNMPIESRLYSDGLFSFSVNVNRATPSSTDQM
                     LRTGRRTVSTSVRDNAEITIVGELPPQTAKRIAENIKFGTAQ"
     gene            18083..18562
                     /gene="rseC"
                     /locus_tag="NBI74_002613"
     CDS             18083..18562
                     /gene="rseC"
                     /locus_tag="NBI74_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR-reducing system protein RseC"
                     /protein_id="HCD1932929.1"
                     /translation="MIKEWATVVSWENGQALVSCDVKASCSSCASRAGCGSRVLNKLG
                     PQTTHTIVVPCDEPLVPGQKVELGIAEGSLLSSALLVYMSPLVGLFLIASLFQLLFAS
                     DVAALCGAILGGIGGFLIARGYSRKFAARAEWQPIILSVALPPGLVRFETSSEDASQ"
     gene            18760..20559
                     /gene="lepA"
                     /locus_tag="NBI74_002614"
     CDS             18760..20559
                     /gene="lepA"
                     /locus_tag="NBI74_002614"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HCD1932930.1"
                     /translation="MKNIRNFSIIAHIDHGKSTLSDRIIQICGGLSDREMEAQVLDSM
                     DLERERGITIKAQSVTLDYKASDGETYQLNFIDTPGHVDFSYEVSRSLAACEGALLVV
                     DAGQGVEAQTLANCYTAMEMDLEVVPVLNKIDLPAADPERVAEEIEDIVGIDATDAVR
                     CSAKTGVGVQDVLERLVRDIPPPEGDPEGPLQALIIDSWFDNYLGVVSLIRIKNGTLR
                     KGDKVKVMSTGQTYNADRLGIFTPKQIDRTELKCGEVGWLVCAIKDIHGAPVGDTLTL
                     ARNPAEKALPGFKKVKPQVYAGLFPVSSDDYEAFRDALGKLSLNDASLFYEPESSSAL
                     GFGFRCGFLGLLHMEIIQERLEREYDLDLITTAPTVVYEVETTSREVIYVDSPSKLPA
                     VNNIYELREPIAECHMLLPQAYLGNVITLCVEKRGVQTNMVYHGNQVALTYEIPMAEV
                     VLDFFDRLKSTSRGYASLDYNFKRFQASDMVRVDVLINGERVDALALITHRGNSQNRG
                     RELVEKMKDLIPRQQFDIAIQAAIGTHIIARSTVKQLRKNVLAKCYGGDISRKKKLLQ
                     KQKEGKKRMKQIGNVELPQEAFLAILHVGKDNK"
     gene            20575..21549
                     /gene="lepB"
                     /locus_tag="NBI74_002615"
     CDS             20575..21549
                     /gene="lepB"
                     /locus_tag="NBI74_002615"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HCD1932931.1"
                     /translation="MANMFALILVIATLVTGILWCVDKFFFAPKRRERQAAAQAAAGD
                     SLDKATLKKVAPKPGWLETGASVFPVLAIVLIVRSFIYEPFQIPSGSMMPTLLIGDFI
                     LVEKFAYGIKDPIYQKTLIETGHPKRGDIVVFKYPEDPKLDYIKRAVGLPGDKVTYDP
                     VSKELTIQPGCSSGQACENALPVTYSNVEPSDFVQTFSRRNGGEATSGFFEVPKNETK
                     ENGIRLSERKETLGDVTHRILTVPIAQDQVGMYYQQPGQQLATWIVPPGQYFMMGDNR
                     DNSADSRYWGFVPEANLVGRATAIWMSFDKQEGEWPTGVRLSRIGGIH"
     gene            21821..22501
                     /gene="rnc"
                     /locus_tag="NBI74_002616"
     CDS             21821..22501
                     /gene="rnc"
                     /locus_tag="NBI74_002616"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004248457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HCD1932932.1"
                     /translation="MNPIVINRLQRKLGYTFNHQELLQQALTHRSASSKHNERLEFLG
                     DSILSYVIANALYHRFPRVDEGDMSRMRATLVRGNTLAELAREFELGECLRLGPGELK
                     SGGFRRESILADTVEALIGGVFLDSDIQTVEKLILNWYQTRLDEISPGDKQKDPKTRL
                     QEYLQGRHLPLPTYLVVQVRGEAHDQEFTIHCQVSGLSEPVVGTGSSRRKAEQAAAEQ
                     ALKKLELE"
     gene            22498..23403
                     /gene="era"
                     /locus_tag="NBI74_002617"
     CDS             22498..23403
                     /gene="era"
                     /locus_tag="NBI74_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HCD1932933.1"
                     /translation="MSIDKSYCGFIAIVGRPNVGKSTLLNKLLGQKISITSRKAQTTR
                     HRIVGIHTEGAYQAIYVDTPGLHMEEKRAINRLMNKAASSSIGDVELVIFVVEGTRWT
                     PDDEMVLNKLRDGKAPVILAVNKVDNVQEKADLLPHLQFLASQMNFLDIVPISAETGL
                     NVDTIAAIVRKHLPEATHHFPEDYITDRSQRFMASEIIREKLMRFLGAELPYSVTVEI
                     ERFVSNERGGYDINGLILVEREGQKKMVIGNKGAKIKTIGIEARKDMQEMFEAPVHLE
                     LWVKVKSGWADDERALRSLGYVDDL"
     gene            23415..24143
                     /gene="recO"
                     /locus_tag="NBI74_002618"
     CDS             23415..24143
                     /gene="recO"
                     /locus_tag="NBI74_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HCD1932934.1"
                     /translation="MEGWQRAFVLHSRPWSETSLMLDVFTEESGRVRLVAKGARSKRS
                     TLKGALQPFTPLLLRFGGRGEVKTLRSAEAVSLALPLSGITLYSGLYINELLSRVLEY
                     ETRFSELFFDYLHCIQSLAGVTGTPEPALRRFELALLGHLGYGVNFTHCAGSGEPVDD
                     TMTYRYREEKGFIASVVIDNKTFTGRQLKALNAREFPDADTLRAAKRFTRMALKPYLG
                     GKPLKSRELFRQFMPKRTVKTHYE"
     gene            24155..24886
                     /gene="pdxJ"
                     /locus_tag="NBI74_002619"
     CDS             24155..24886
                     /gene="pdxJ"
                     /locus_tag="NBI74_002619"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HCD1932935.1"
                     /translation="MAELLLGVNIDHIATLRNARGTAYPDPVQAAFIAEQAGADGITV
                     HLREDRRHITDRDVCILRQTLDTRMNLEMAVTEEMLAIAVETKPHFCCLVPEKRQEVT
                     TEGGLDVAGQREKMRDACKRLADAGIQVSLFIDADEEQIKAAAEVGAPFIEIHTGCYA
                     DAKTDAEQAQELARIAKAATFATSLGLKVNAGHGLTYHNVKAIAAIPEMHELNIGHAI
                     IGRAVMTGLKDAVAEMKRLMLEARG"
     gene            24886..>25214
                     /locus_tag="NBI74_002620"
     CDS             24886..>25214
                     /locus_tag="NBI74_002620"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HCD1932936.1"
                     /translation="MAILGLGTDIVEIARIEAVIARSGERLARRVLSDNEWEIWKTHH
                     QPVRFLAKRFAVKEAAAKAFGTGIRNGLAFNQFEVFNDELGKPRLRLWGEALKLAEKL
                     GVVNMHVT"
BASE COUNT         6177 a         6286 c         6670 g         6081 t
ORIGIN      
        1 aaaggcccag tcggaagact gggccttttt gcatatgtgc ggaataaatg ccggatgcga
       61 cgctggcgcg tcttatactc ccacataagc cagattcaac agcgaatacg tcttccccaa
      121 attgcccact tccatacttc ctccttacca gaaatctatc cttaagctcc ttaataacca
      181 ttttcctgct aactaaattc atggttaagg ttgtataatg atgcgcaaca aatgtataac
      241 attttcttta ccaaaaaaaa ataaacaaaa gcgaccgaca aaagcatcgg attacggcag
      301 gagacataat ggcatggctg aaagtactgt aacggcagac agcaaactga caagtagtga
      361 tactcgtcgc cgcatttggg caattgttgg ggcctcttca ggtaatctgg tcgagtggtt
      421 cgatttctat gtctactcgt tctgttcact ctacttcgcc cacatcttct tcccttccgg
      481 gaacacgacg actcaactac tgcaaacagc aggtgttttt gctgcaggat ttctgatgcg
      541 cccaataggc ggttggctgt ttggccgcat agccgataaa catggtcgca aaaaatcgat
      601 gctgttatcg gtgtgtatga tgtgtttcgg ctcactggtt atcgcctgtt tgccaggcta
      661 cgaaacaatc ggtacctggg ctccggcatt attgcttctc gcgcgtttat tccagggatt
      721 atccgttggc ggagaatatg gcaccagcgc cacctatatg agtgaagttg ccgttgaagg
      781 gcgcaaaggt ttttacgcat catttcagta tgtgacgttg atcggcgggc aactgctagc
      841 tctactggtt gtcgtggttt tacaacacac catggaagac gctgtactca gagagtgggg
      901 atggcgtatt cctttcgcgt taggagctgt gttagctgtt gtggcgttgc ggttacgtcg
      961 tcagttagat gaaacttcac aacaagaaac acgcgcttta aaagaagctg gatctctgaa
     1021 aggattatgg cgcaatcgcc gtgcattcat catggttctc ggttttaccg ctgcgggctc
     1081 cctttgtttc tataccttca ccacttatat gcagaagtat ctggtaaata ctgcgggaat
     1141 gcatgccaac gtggcgagtg gcattatgac tgccgcattg tttgtattca tgcttattca
     1201 accaattatt ggcgcgctgt cggataagat tggtcgccgt acctcaatgt tatgtttcgg
     1261 ttctctggca accattttta ccgttcctat tctctcagca ttgcagaacg tttcctcgcc
     1321 ttatgccgct tttggtctgg tgatgtgcgc cctgctgata gtgagttttt atacttcaat
     1381 cagtggaata ctgaaggctg agatgttccc ggcacaggtt cgcgcattag gcgttggtct
     1441 gtcatatgcg ttcgccaatg ctatatttgg tgggtcggcg gagtacgtag cgttgtcgct
     1501 gaaatcaata ggaatggaaa cagccttctt ctggtatgtg accttgatgg ccgtggtggc
     1561 gtttctggtt tcactaacat tgcaccgtaa aggaaaaggg atgcggcttt agtgacgggt
     1621 cagttgccag acggtatagc cggtgcttgc accggcgata tcccaggcaa aatccttcca
     1681 gctccagccg ctcccctcag ggcggctatc ccaaagctct tttgacgccc ccagactgac
     1741 agagaacatc aatccaaaca tggcactgcg atcccggctc atcccctgat gctgtgaata
     1801 ttcatttccg gcggcggaaa gcatcgccga ggcgataaag tgttgagctt tatcctgccc
     1861 gctccagtta tcgttagcca tatggctgca tccagaaagt aacaatagcg aacagacaaa
     1921 aagaatacgc aaaacgtggc ctccaaaaaa caaaccccgt acaggacggg gttgtgatta
     1981 caggatgcgg ctaattaatc ggtcgatgcg gatacggcgc aaacggcgga tgagtttacg
     2041 aaccttcacc ggataatcgg caatactttg cagatcgcgg tagtgcttaa cgatggtggt
     2101 atgctcgcgg atcagatcca gttctttctc tcgctgtggc gcaagctcca gttgcggatc
     2161 gtggatcaaa atggcgtttt ccagatccag acgccaggcg cgcgggttca ggttattacc
     2221 ggtgatcaaa atccacttat catcaaccca catccctttc aggtgatagg tgttgtcgtc
     2281 atctttccat aaccgaacta ctaactggtc agtgttgacg taatactgta aacgactcag
     2341 gaaacgacgc agattgatct catagagata aggcaatgcg ccaattatct tgaaaggttc
     2401 atcttccgga atgtagaagt cattcgcggt tttatcacca acaataattt cgaccttttt
     2461 cccttcgcgc agcaactgga taatattgcg cacaaggatt gctggtaggt tgaagtaagg
     2521 tgtgcagatg gttagtttct gctcggcaca aggcataaga tggaaaatag tcttgttcaa
     2581 cagactcgat ttccccagcc ccactagcgg cgttacggaa agctgatcgt tgtcggcatc
     2641 gccctggaaa tgataagcgg catcacgcag ctcctggcgg aacaggcgaa tgtcgttctt
     2701 gatttccggg ctttttggcc gattaacatc atccagacga ttaacgccgc ggccattcat
     2761 aatattctgt gtaacccatt caaacataat gtctgacatc ttacggttac ggatcagatg
     2821 ataacggtcg tagcgatatt tatcgtgctg atgcaggtaa acatcgttca ggctggcacc
     2881 gctataaagt acgctatcgt cgatgataaa gcctttaaag tgcagaacac caagggcttc
     2941 acgggtattg attggaacgc cataaaccgg aacatttacg cccggatttt cctgcgccat
     3001 gcggcagtac cagtcagcgt tagtgttaga tgccgcagcg ccaatgcgtc cacgttgtgc
     3061 acgatgccag tcgaccagca cccgcacatc cagttccgga cgctgccttt tagcctcata
     3121 caacgcgttc agaatgcctt tgccaccgtc atcctgttcg agatacaggg caacaatgca
     3181 aatgcgctgc ttcgcgctgg ctattttttc cagcaacgtc tcccggaagt cggcgggagc
     3241 gtaaaagaaa tcgacatcat caactgattg agaaatcttg ggtagttggg caaggtgttg
     3301 ttgatgttta ttacgcttaa attttgacaa catcacagtg catttcttct ctgttcattg
     3361 aagggtcctc tgtgcaatgc agacgacata agcgggcaat aataacacca gtcccgatta
     3421 agtggtcaac atttccagta ccttactcat gattcctcgc gctgggcaag acttagcgta
     3481 agcccaacga tcccctcttc gagctggata tcaacgttaa acccgagctt gcgggccagc
     3541 gccaccatgc cacgattgtt tggcatcgta ataccattca gacgttgtag tccgtgatct
     3601 cgcgtatagg taatcaactt ttccattaag cgtcgaccta agcctaaccc tttaagatcc
     3661 gagcgaacca gtacggcaaa ttcggcatcg atgttatcag ggtcggagat cgcacgtgtg
     3721 acgccgagga tctcttccgt ttgatcaatt cgtcgtaccg ctacaaatgc catttcccga
     3781 tcgtagtcga tctgcgtcat gttggctaaa tcttcatggg taaattcgtt gatctcgcta
     3841 aagtagcggt aataaagatc ttctttggtg actcgcgaaa tgaattgctg aagctgtggc
     3901 tcatcttctg gcaaaatcgg gcggaacaag cagcgttcac cgtttttcaa ttctacccat
     3961 tcttccagct gatgcggata agggcgcaca gccagtcggc tctcgttatc accttcaaac
     4021 ggcgcgatat ccagcgtgac atccagcgcg gtaaattcac tgccagaagc cagcaaagga
     4081 tgaatatcca gacgctgaat ttccgggcaa tcgacaatca agttggaaac ctgcaccaga
     4141 agctggctca agcctgcaac atccaatggg cgtagcgcac tgcgcgcacg aatcttttta
     4201 cttttgatcc cctgaataac cagatagcgg gccaggttca tgttcagcgg cggcagtgcg
     4261 acgacggctt gatcttcagg acgccactcc acaccgcctt cacccagcat gatcaacggc
     4321 ccgaaaaccg gatcgtgctc aaccacaacc cgcaactcct gagcgccagc acggttagcc
     4381 atactttgca ccaacaggcc gtggacccgc gcctgcggcc aggccatttt tacgcgatcg
     4441 aaaatagcgt ttgccgcttg ctggacttca ttggctgtac gtaggtagag catgacgccc
     4501 tgaacttccg atttatgtgg aatatccggc gagcgcaatt tcagtgccac cggatagcca
     4561 atctgttcgg caatatgtac cgcttcggtg ctatcactgg caatccaggt agggagagta
     4621 ttcataccat acgcttgcag gatgggctga acttcatggg tatcgagcga cgtagcccct
     4681 tcggcaatcg cctgttgcaa cagaagatgc gcttctgcgg tattggaagt cagattgctg
     4741 ggcaacgccg gcgtttcgcg tagttgcttc tgattacgcc ggtactcgac catatgcata
     4801 aaagcagtga tggttccttc cggagtacgg taggtcggca gcccggcttc gctgaataaa
     4861 cgtcgtgcct cttgcgagga gtgctcgccg caccagttcg tcagcaaaga gacatatttg
     4921 ctgcggggat gatgctttac cgcttcaatt aatacttgcg cgctttctgt tgcgggagca
     4981 gcggcgctgg gcgaatgaat aaccatcagc gcatcaaaat cctggctgtg gagcagaatg
     5041 tccagcgttt tgacataatg ctcgctgctg gcgtcatcgc gcagatcgag cgggttagag
     5101 attgccacat gccctggcag tgcatcgcgc agtttctggc aggtttcttc gcttagcgtt
     5161 gccagcttgc cattgcgtga ccataaggca tccagcgcca gcgcggcagg cgcagcaccg
     5221 ttgctgataa tcatcaaccg gtcgccacgc agcgggcgca tatggctaag ggtttccacc
     5281 gccgaaaaca gttcgtgggt gtcctgtacc cgcaacaaac cggcacgctg aatagctgca
     5341 tcccacgccg gatccattcc tgccgtggtg ttcaacagac gttgagccgc cgggctacgt
     5401 ccgcttttaa tcaccaaaat cggtttattg cgcgaggcac tacgggctgc cgaaacaaag
     5461 cgtcgcgcgt cgcttaactg ttcgagatag agcaggatgg cgctggtttt actgtcgcgc
     5521 gccagatagt cgagcaattc atcaacgtcg atatccaggc tgtcgccgag cgcaataaag
     5581 taggaaaagc ccatctcacg ctgttgcgcc cagtcgagga tggtgttgga gacagcagcc
     5641 gattgcgaaa taaacgccag cttgccgcgt ttaatcggca caggcgaaaa gctggcattc
     5701 agaccttgcc agggagccag taagcccaga ctgtttggtc ctagcaggcg catgttatgg
     5761 cgcagggcgc aggcgcggag atcttcgtgt tgcgatgccg gggcggaaag aataatgcag
     5821 gttttacagc ctttctcgcc gagctcttcc agaagagcaa gattacggct ggcattggta
     5881 cataaaaccg caaggtcggg tgtaaagggc aagttggcaa tatccggcca ggccaacaca
     5941 cccaacaccg ctttccaggc tggcgtcacc gggagtaccg gtccgttaaa gcctcccgcc
     6001 agcaggttac gcatcatcag gtaacctgcg cgattgggtt tcatcgatgc gccaattacc
     6061 gcaatcgatt ttggtcgcag tagtgcttcc agtcctcgct gactcatacc ggtctcccgc
     6121 agaagtgacc gaatgatttt aaacgctttc taactgttct gctgtgatgc ttcccggatg
     6181 ttgcgttttt cctgccagat agcgtgtttt aaagcgggta aaatgctcgc ctaaccctgt
     6241 cgccgccctg gtatcgccgg ccatatctaa cagtgcgatg gctacctcgg cagtacaata
     6301 ttggccttca gcctgggctt cacgcaggcg ataggcagaa agccgagaaa gatcgacgga
     6361 aatgacggga agattatcca gatacggact tttacgaaac atcttgcgag cttctggcca
     6421 ggtaccatcg agcatgataa atagcggagg cttatcggca ggtggtgtga agatcacttc
     6481 ccgttgctca tcagcatacg aagcgggaaa gaccaccatt ggctgatagt ctgggttttg
     6541 caccagatcc agcaaatctt gcgagggttc ggtacgcgac cattgaaacg caacggtatc
     6601 aggcaaaata tcagcaatca gacgcccggt attactgggc ttcattggct cggtgtcgaa
     6661 catcagcaaa cagaagtgac tttttgcttg tgctggggta attgtcgaac agagacataa
     6721 tttctctggc aaaagacagc gttggcagcg acgaacgcga ttaccgcggg caagaaaagg
     6781 acgtgttgcg cgcgcaatac gctcggcgcg taactggaga acagcgtttt cggtcataag
     6841 agagcgtcga aaaaacgcca ttgtcgcaga ggagaaaacg gggcacaaga tgcgccccgg
     6901 taagattaaa gagattcgtt cagccagctg tcgaacggcg ctttcggtac tgcgccatta
     6961 agcatgtcga caacctgacc gtttttgaaa atcatgatcg tcggtatact acgaattcca
     7021 aagcgactgc tcaattcacg ttcagcttcg gtattcactt tcacaaagcg cactttaccg
     7081 ctacgctctt gcgcgacatc ttcaaaaatt ggtgcgaaat tacggcaggg gccgcaccac
     7141 ggtgcccaga agtcgatcac cacaggtaga tcatctttca gcaatttgtc gagcgtttca
     7201 ccggtcgcat taatcacctc tccgtcaaac aagtcgtgac cgcagcgtcc gcattttgcc
     7261 gcatcttcga tccgatcgtc gggaatgcga ttgatggcct gacaatgggt acaaacggta
     7321 ttcataacta acctcgggat gagtaaggag acttatcggc aacgacgccg gtatgtttct
     7381 aatatgttac atattatcgg ggacattgtt ccagacaaca atctgtttta ttcaatgggt
     7441 atgatagaca caggctttgg aacgaaatca tggcgaaccg cgcgcacttc gggtaatctg
     7501 cgcgcttcgc gcagcgctgg tggagaaaag catgaacgat gaaatgaaag gtaaaagcgg
     7561 caaggtcaaa gtgatgtatg tccgcagtga tgatgattct gataaacgta cccacaaccc
     7621 gcgtaccggg aaagggggcg ggcgtccagg aaaatctcgt gctgacggtg gccgtcgccc
     7681 cgcccgcgat gacaaacaga gtcagcaccg tgaccgcaag tgggaagatt cgccgtggcg
     7741 cacggtttcc cgcgcgccgg gtgatgagac gccggaaaag gccgatcacg gtggcatcag
     7801 tggtaaaagt tttattgatc cggaagtttt gcgtcgtcag cgtgcggaag aaacccgcgt
     7861 ctacggcgaa aacgcctgtc aggcactgtt ccagagccgt ccggaagcga ttgttcgcgc
     7921 ctggtttatc cagagcgtaa cgccacgttt taaagaagcc ttgcgctgga tggcggcaaa
     7981 ccgcaaagcg taccatgtgg tggatgaagc ggaactgaca aaagcgtcag gcacggaaca
     8041 tcacggcggc gtttgcttcc tgatcaaaaa gcgtaacggt acaaccgtgc agcagtgggt
     8101 aagccaggca ggcgcgcagg attgtgtact ggccctggaa aacgaatcta acccgcataa
     8161 cctgggtggc atgatgcgta gctgtgcgca cttcggcgtg aaaggtgttg tggtgcagga
     8221 tgcagcactg ctggagtctg gggcggctat ccgtaccgca gaaggcggcg cagagcacgt
     8281 tcagccgatt actggcgaca acattgttaa cgtgctggat gatttccgtc aggcgggtta
     8341 caccgtagtg acaacttcca gcgagcaggg taaaccgctg ttcaaaacca gtctgccagc
     8401 gaaaatggta ctggtgctgg gtcaggaata tgaagggtta ccggatgccg cacgcgatcc
     8461 gaacgatctg cgcgtgaaga ttgatggtac tggcaacgtt gccgggctga atatctctgt
     8521 cgcaaccggc gttcttcttg gcgaatggtg gcgtcagaat aaagcctgat aaatcagccg
     8581 ggtggcaact ctgccatccg gcattttccc gcaaatttac tcactctctg ccggtaatac
     8641 tggcatccag tcaatcggcg tctcgccacg ttgttccagc cactgatttg ccagcacaaa
     8701 atggttgcag ccaaagaatc cacgatgcgc cgaaagcggc gacggatgcg gtgctttcag
     8761 tacatgatgg cgttgcttat ctataatcgc ccctttcttt tgcgcatgtg atccccacaa
     8821 caaaaacacc acgccttcgc gatgctgatt tatgaggctg atgactttat cagtaaacgt
     8881 ctcccagccg aggctggcgt gggaatgcgc ctgaccagcg cgaaccgtca agacggtatt
     8941 gagcagcaga acaccctgac gcgcccagct ttcaagataa ccatgattag ggcgggtgaa
     9001 accgggaata gtattttcca gctctttata catattcagc agtgacggtg gtgtcgcaat
     9061 gccggggcga acggaaaatg ccagaccatg cgcctgtcct ggtccgtgat aaggatcctg
     9121 accgagaatc accactttaa cgtcacccaa ctccgtaaag cggaacgcgt taaagacatc
     9181 tttttgtggt ggatagatag tgacgccgga ctgccgctcg ctggcgacgg tctgaagggt
     9241 attaagaaaa tagggttgct gcttctcttc agccagcaca tcatgccagg ttaattcgtt
     9301 agccatctcg ctctcctgcg aatctttaat ccgcctagct taactgcttc tttctacaga
     9361 acaaaatcat gctcatttta tagacaaaaa gacatgcaaa aaaatgtaaa ttggtaaaat
     9421 ttcacatctg cataagttga tgtaaaacaa taaaatccat ttgtgaccac acttgatatg
     9481 tggtttttat tgatttaaat caaagaatca agggtgtttg aggagtatat atacactcaa
     9541 gcaacaatgg ttttaccaat tggccgcgac aggctgaaca aatcaaataa ttttgccggg
     9601 gaggcatcac atgattacag gtatccagat tactaaagcc gctaacgacg atctgctgaa
     9661 ctctttctgg ttgctggaca gcgaaaaagg cgaagcgcgt tgcatcgttg cgaaagcagg
     9721 ttttgcagaa gacgaagtgg ttgcagtaag caaactgggt gacattgaat accgtgaagt
     9781 tccagtagaa gtgaaaccag aagttcgcgt tgaaggtggt caacacctga acgttaacgt
     9841 tctgcgtcgt gaaactctgg aagatgcagt taagcatccg gaaaaatatc cgcagctgac
     9901 catccgtgta tccggttatg cagttcgctt taactctctg actccggaac agcagcgcga
     9961 cgttatcgct cgtaccttta ctgaaagcct gtaaggctta agtagcaagt tactaagcgg
    10021 aagagggcga cctcttccgc tttttttcgt taatagaaaa tgcgtaccgc gcaatagacc
    10081 agcaacaggg caagcacgat atttaactgg cgaccatact ggcgaaacaa tcgctgaaac
    10141 agatgccccg ccagcgccca gcacacattg ccaaacgtcc caatcatcgc cagcaaaacg
    10201 ctgacgccaa ctacccagct tagcgcctgt gtttgcggca gaacaaacgt cgacagtgcc
    10261 gtaacaccgt acaaaatgat tttgacgttc acaaactgca aagcaaagct ggcccaaaag
    10321 ctgattggtt ttgcctgaag tccgtcttcc tttgttgggc tggtggcgat tttccacgcc
    10381 agccagacaa tatatgccgc ccccgcccaa ctcaaaaggt gtaccgctgc cgggtcaatc
    10441 actgccagtg aaaatgaaat gcccgcacac agtaacatca caatcaaaaa tcccagactc
    10501 atccctgcca gcacacgggt actttgacga aatccatgcg acgtagcaga gctaagggcg
    10561 agaatattgt tcggtcctgg cgtcatggcg gtaatcaggg tgtaagtcca aaaagcactt
    10621 aaaagggtcg gtgtcacttg ttatctcctc tgttttggta gacagtaccc gacacggaac
    10681 atttaaaaaa atgaatgttt ttacattcta aattcgatat tttcgatagg tttggggtta
    10741 tggatctgcg ccgttttatt acgcttaaaa ccgtggtgga agagggttcc tttttgcgag
    10801 cttcgcaaaa attgtgctgt acacaatcga cggtgacttt tcatattcag cagcttgagc
    10861 aggagttctc ggtacaattg tttgagaaaa ttggtcgacg aatgtgcctt acccgcgaag
    10921 gaaaaaagct gctgccgcac atctatgaac ttacccgagt gatggatacg cttcgtgagg
    10981 ctgctaaaaa agagtcggat ccggacgggg aactacgcgt tatttcaggc gaaacgttgc
    11041 tctcttatcg aatgccgcag gtgttgcagc gttttcggca acgcgctcca aaagtgcgtt
    11101 tatcgttgca gtcgctgaac tgttacgtga tccgtgatgc gctactgaat gatgaggccg
    11161 atgtcggggt tttttatcgc gtagggaatg atgatgctct gaaccgacga gagttgggtg
    11221 aacaaccgct ggtgctggtg gcttcaccgc aaattgcaga tgtcgatttt actgagccgg
    11281 gaagacataa cgcctgtagc tttattatca acgaaccaca atgtgtcttc cggcagatat
    11341 ttgagagcac gctgcgccag cggcggatca cggtggaaaa caccattgag cttataagta
    11401 tcgaaagcat caagcgttgt gtcgcggcaa atatcggagt cagttacctg ccgcgttttg
    11461 cggtagtaaa agagttggag tgcggagaat taattgaatt gccttttggc gaacagtcgc
    11521 agaccattac ggcaatgtgc gctcaccatg ccggaaaagc ggttagcccg gcgatgcaca
    11581 cttttattca gtgtgttgaa gagtgttttt tgccgggata aaaacaatgc cgggcttaaa
    11641 cccggcattg agattactct tctgtcgttt gcggtggcac gcccgttccg cttggtttac
    11701 ggcgcttacc aatattttta gtgtcgcgat ggcgtttttt cacccgcggc ttctctttct
    11761 ctttagcttt tttcttctcg gcacgtttag ccaacacttt cttcgatggc ttgccggtct
    11821 gcttctcgct tggcgcacgc gttttcggac gtaactcatc aattacgcga gctttaattg
    11881 gctcttcaat ataacggcca actttgccca gcagcagatg gtcatgggct tccaccagcg
    11941 aaatcgcggt gcctttacga ccggcgcgcg cggtacgtcc gatacggtgc aaataagtat
    12001 cgccactgcg cggcatatcg aagttaaaga cgtggctgac gtcaggaatg tcgataccgc
    12061 gcgcggcaac atcggtagca accagtacgt ttacgcggcc ttcggtcaag cgtttaatcg
    12121 cttcgttacg cttgccttgt accatctcac cttcgagata gcagttgttg atgcctgctt
    12181 cgcgcagcca gtttgccagc tcatgcacac gctcacgctt acgcacaaac acaattgagc
    12241 gggtcgcttc cggctgtttt aacagatgca ccagcaacgc ggttttatgc tcaagatcat
    12301 cggcgcggta ataccactga tgaatttttt tgcgctcacg ggtggacgga ttggcagaaa
    12361 cttccaccgg atcttccaac agacgctcgg caaagtcctg aatcgcatcg ccttccagcg
    12421 ttgccgaaaa gagcagggtc tgtttacgcc agcgcgtttc gccagcaata tgttcgatat
    12481 cctgggcgaa gcccatatcc agcatacggt ctgcttcgtc gaggatcagc gtttcaaccg
    12541 cgcggcaatc gaagttctct tcttttatgt attgcagcag acgtccggtc gtggcgacca
    12601 caatgtcctg gttttcgctg aacacttctg cgtggttcat ataggccacg ccgccggtga
    12661 tggtggcaat atccagatgc gtatgtttcg ccagttcgcg ggcatgatcg gccacctgca
    12721 tcgccagctc gcgagttggg gtgaggatca aaatacgcgg cggaccggat ttcttacgtg
    12781 ggaaatcgag caggtgctgc aacgctggca gcagatacgc cgccgtttta ccggtgcctg
    12841 tcggcgcaga accgagtaca tcacggccat cgagcgcagg cggaatggcg gcagcctgaa
    12901 tggcggtcgg gcgagtgaaa cctttatcct ggagggcttc cagcaggctt tcgtcgagtt
    12961 caagttcgga aaaagtcgtt acagtcatgt tctacctctg tgtggggcgc tgattataga
    13021 cgttacggct gcaatcttca tctgtttgta tggatatcgc ttctcgggta ttgttctcac
    13081 ccggtgctat cgccgttttt cctgcaaggt tttattttca tgtcacagtc tacatccgtg
    13141 tttcgtcgta atggatttac ttttaaacag ttttttgttg ctcacgatcg ctgtgcgatg
    13201 aaagtgggaa cggatggcat tttgctgggc gcatgggcac cggtagctgg ggtaaaacgt
    13261 tgccttgata tcggcgcggg tagcgggttg ctggcattaa tgctggcgca gcgaacagat
    13321 gacagcgtga tgattgatgc agttgaactg gaaagtgaag ctgccgcgca agcgcaggaa
    13381 aatatcaacc agtccccgtg ggcagagcgg attaatgtcc atacggcgga tattctgcag
    13441 tggatcacac agcagacagt acgcttcgat ttaatcatca gcaacccacc ttactatcag
    13501 cagggagtag agtgcgcgac acctcaacgg gaacaggctc gctataccac tacgcttgat
    13561 cacccatcgt tgctgacttg tgcggcggag tgcattaccg aagagggatt tttctgcgtg
    13621 gtgctgccag agcagattgg taatggtttt actgaactgg cattaagtat gggctggcat
    13681 ttacgtttgc ggacggatgt ggcggaaaac gaagcgcgac tgccgcatcg ggtgctgctg
    13741 gcattctccc cgcaggcggg agagtgcttt agcgatcgct tagtcattcg tgggccagac
    13801 cagaactatt ccgaagcgta tacggcgctg acccaggctt tttatctgtt tatgtaatga
    13861 ttgccggcag aaaggatcga tggaccggaa tgcgtgagca gttccggata atccagcgtg
    13921 aaatgcaggc cacggctctc cttgcgcatc atcgcacagc gaacaatcaa ctcggcaacc
    13981 tgtaccagat tacgcagctc cagcaaatta tttgagacgc ggaaatgggc gtaatattcg
    14041 tctatttctt gttggagcat ggttatccgc cgcagggcgc gttccaggcg cttcgttgtg
    14101 cgaacaatgc caacgtaatc ccacataaac agacgtagct cgtgccagtt atgctgaatt
    14161 actacccgtt cgtcagggtt ctcaacgcgg ctttcatccc acggcggtaa cgtactgacg
    14221 ccgtgggcat aaggtatacg tctgctgata tcttctgccg ccgaccagcc atagaccaga
    14281 cactccagca atgaattcga ggccatgcgg ttagcgccgt gtaagccggt ataactcact
    14341 tcgccaatgg catacaaacc ctcgacgtcc gtacgcccat gatcatcaac cattacacca
    14401 ccgcaggtat aatgtgcagc aggcacaatc ggcacgggtt ctttcgtgag atctatcccc
    14461 agcccgagca atttttcata aatcatcggg aaatgctggc gaataaaatc ggcgggctta
    14521 tggctgatgt cgaggaacat acaatctgcg ccgaggcgtt tcatttcatg gtcaatggcg
    14581 cgggcgacaa tatcgcgcgg ggccagttcg ccgcgctcat caaaatcggg cataaaacgc
    14641 gtgccatccg ggcgcttgag ataagcgcct tcgccgcgca gtgcttccgt taacaggaaa
    14701 ttgcgtgcct gtgggtgata cagcgcggta gggtggaact gattaaattc gagattggca
    14761 acccggcagc ctgcgcgcca cgccatagca atgccatcgc cagaagaaat atccggattg
    14821 gtggtgtact gataaacttt tgaagcaccg ccggttgcca gtaccactgc ttttgcgtgg
    14881 caggtttcaa ccgtttcttt atttcggttc catacccacg cgccaacaac ccgtcgcgtg
    14941 cccggcaggc caattttgtc agaaacaatc agatcaaccg cgttgctgcg ctccagcacg
    15001 cgaatattcg gatggttctg cgccttgccc accagcgtgg tttctacttc tctaccggtg
    15061 gcgtcggcgg catgaagaat acgacggtga ctatgtccac cttcacgagt cagatggtag
    15121 ctttcttcgc cattcggctg aatgtgggta tcaaacaaca ccccctggtc gattagccat
    15181 tgcacacagg atcgtgcatt actggcgaca aattcaactg catggcgatc gcaaatacca
    15241 gccccggcaa tcaatgtgtc ttccacatgc gagtcaatgc tgtcagtttc atcaaacacg
    15301 gcggcaatac cgccctgggc ataaaatgtt gaaccttccg ttaccgggcc tttacttaga
    15361 acgatgacct gatgctggtc agccaggcgt agcgccagtg aaagtccagc tgcgccgcta
    15421 ccgataatca acacgtcaca tgaatgttca gggagagtat tcattttctt tgtttaattt
    15481 actaaacact gtttggtcag cataacatca tgttgtgcaa ataaacacct gctattttaa
    15541 tatttgttac agtgactaaa cacgctggct cagggcggcg agaaaaagag aagttactgg
    15601 ctggtggagg attaggtggt gaaataaaaa ggccgttggg ttactcttca ggcagttaaa
    15661 tgggcatttc tacacagata atgcgatgtt cagattctgt agacttataa tgatagataa
    15721 tgatccgtct acagcatgac aaacaaaaac agatgcgtta cggaacttta caaaaacgag
    15781 acactctaac cctttgcttg ctcaaattgc agctaatgga gtggcgtttc gatagcgcgt
    15841 ggaaatttgg tttggggaga ctttacctcg gatgagcgag cagttaacgg accaggtcct
    15901 ggttgaacgg gtccagaagg gagatcagaa agcctttaac ttactggtag tgcgctatca
    15961 gcataaagtg gcgagtctgg tttcccgcta tgtgccgtcg ggtgatgttc ccgatgtggt
    16021 acaagaagct tttattaaag cctatcgtgc gctggattcg ttccggggag atagcgcttt
    16081 ttatacatgg ctgtatcgga ttgctgtaaa tacagcgaaa aattacctgg ttgctcaggg
    16141 gcgtcgtcca ccttccagtg atgtggatgc cattgaagct gaaaacttcg aaagtggcgg
    16201 cgcgttgaaa gaaatttcga accctgagaa cttaatgttg tcagaagaac tgagacagat
    16261 agttttccga actattgagt ccctcccgga agatttacgc atggcaataa ccttgcggga
    16321 gctggatggc ctgagctatg aagagatagc cgctatcatg gattgtccgg tgggtacggt
    16381 gcgttcacgt atcttccgag cgagggaagc tattgataac aaagttcaac cgcttatcag
    16441 gcgttgacga tagcgggata ctggataagg gtattaggca tgcagaaaga acaactttcc
    16501 gctttaatgg atggcgaaac gctggatagt gagctgctta acgaactggc tcataaccca
    16561 gaaatgcaga aaacctggga aagctatcac ttaatccgtg actcaatgcg gggtgatact
    16621 cccgaggtgc tccatttcga tatctcttca cgcgtgatgg ccgccattga agaagagcca
    16681 gtacgtcaac cggcgacatt gatcccggaa gcccagcctg cgccgcatca atggcagaaa
    16741 atgccattct ggcagaaagt acgtccgtgg gcggcacagc ttacccaaat gggcgtagca
    16801 gcatgcgtat cgcttgcagt tatcgttggc gtccagcact ataatgggca atctgaaacg
    16861 tcccagcagc ccgaaacgcc agtatttaat acactgccga tgatgggtaa agccagcccg
    16921 gttagtctgg gagtaccttc tgaagcgacc gcaaataatg gtcaacagca gcaggtacag
    16981 gagcagcgtc gtcgcattaa tgcaatgttg caggattacg aactgcaacg ccgactccac
    17041 tctgaacagc ttcagtttga gcaggcacaa actcagcaag ccgctgtaca ggtgccagga
    17101 attcaaactt taggaacgca atcgcagtaa tgaagcaact ttggtttgcc atgtcattag
    17161 tgacaggtag cctgttattc tctgctaacg ccttggccac tcccgcgtcc ggggcgttat
    17221 tacagcagat gaacctggcc agtcagtcac tgaattacga gctgtcattt atcagcatca
    17281 ataaacaggg tgttgagtct ctgcgttatc gacatgcacg cctcgataac cgtcctcttg
    17341 cacaattgtt gcaaatggat ggcccgcgcc gggaagtggt acagcgcggc aatgaaatca
    17401 gctattttga accgggactt gaaccgttca cgcttaatgg cgattacatt gttgattctc
    17461 tgccatcgct catctatacc gatttcaaac gcctttctcc ttactacgac tttatctctg
    17521 tcgggcgcac gcgtattgct gatcgtcttt gcgaagtcat tcgtgtggtt gcccgagatg
    17581 gtacacgcta cagctacatc gtgtggatgg acaccgaatc gaaattaccg atgcgggttg
    17641 atctccttga tcgcgatggt gaaacgctgg aacaatttcg cgtgattgct tttaacgtca
    17701 atcaggatat cggcagcagt atgcagacgc tggcgaaggc aaatttgccg ccgttgcttt
    17761 ctgttcctgt tggtgaaaaa gctaaattca gctggacgcc aacctggctg ccacagggtt
    17821 ttagcgaagt ttccagtagt cgacgtccgc taccgacgat ggacaacatg cctatcgaat
    17881 cacgtctcta ttccgacgga ttattcagct tctcggtaaa cgttaaccgc gctacgccat
    17941 cgagcaccga tcagatgttg cgcaccggac gcagaaccgt cagtacaagc gtacgtgata
    18001 acgccgaaat caccattgtt ggtgaactgc cgccgcaaac ggcgaaacgc attgccgaga
    18061 atattaagtt cgggacagcg caatgatcaa agagtgggct accgtcgtct cctgggaaaa
    18121 cgggcaggcg ctggtcagtt gtgatgttaa agcctcatgc agcagctgcg cttcacgcgc
    18181 cggttgcggt agccgtgtgt tgaataaact tggcccgcaa accacgcata ccattgtcgt
    18241 accctgtgat gagccgctag tgccggggca aaaagtggaa ttggggatcg ccgaaggcag
    18301 ccttcttagc tccgcattac tggtttatat gtcgccgctg gtgggattat tcctcatcgc
    18361 ttcgctattt cagctactct ttgcttctga cgtcgcagca ttatgtggtg cgatcctcgg
    18421 cggtattggt gggttcctga ttgcgcgcgg ctattcgcgt aagtttgctg cccgggcaga
    18481 atggcaaccg atcatcttaa gcgtggcatt gccgcctggc cttgtgcgat ttgaaacatc
    18541 ttcggaagac gcgagccagt gatttcactg gctttgttgc attttgcata acaaatgcga
    18601 ggacgtttcc atataccaca atccatacgg tatgaacatt tcctcgcctc aatgttgtag
    18661 tgtagaatgc ggcgtttcta ttaatacaga cgttaagctc agaacagcga cttctaaaag
    18721 cctggttaac cgggcattaa ggcacaataa tcatacttta tgaagaatat acgtaacttt
    18781 tcgatcatag ctcacattga ccacggtaaa tcgacgctgt ctgaccgtat tatccagatc
    18841 tgcggtggcc tgtctgaccg tgaaatggag gcgcaggttc tcgattccat ggatcttgag
    18901 cgtgagcgtg gcattaccat caaagcgcaa agcgtgacgc tggactacaa agcgtctgac
    18961 ggcgaaacct atcagcttaa ctttatcgac accccgggcc acgtagactt ctcctatgaa
    19021 gtttctcgtt cgctggctgc ctgtgaaggt gcattgctgg tggtcgacgc cgggcagggc
    19081 gtagaagcgc aaaccctggc aaactgctac accgccatgg aaatggatct cgaagtggtg
    19141 ccggtactga acaagattga cctgccggca gccgatcctg aacgcgtggc ggaagaaatt
    19201 gaagatatcg tcggcatcga cgccaccgac gcggtgcgct gttcagcgaa aaccggcgtt
    19261 ggcgtgcaag acgttctcga acgcttggtg cgcgacattc cgccgccgga aggcgatccg
    19321 gaaggcccat tgcaggcgct gattatcgac tcctggttcg acaactacct gggcgttgtt
    19381 tcacttatcc gtattaaaaa cggcaccctg cgtaagggcg acaaagtgaa agtcatgagc
    19441 accgggcaga cctataacgc cgatcgtctg ggcatcttca cgccgaagca gattgaccgc
    19501 actgaactga aatgtggcga agtaggctgg ctggtatgtg cgattaaaga tatccacggc
    19561 gctccagtag gcgatacctt aacactggcg cgtaatccgg cagaaaaggc gctgcctggc
    19621 tttaagaaag tcaaacctca ggtatatgct ggtctgttcc cggtaagttc cgacgactat
    19681 gaagccttcc gtgacgcgct gggtaaactc agcctgaacg atgcctcact gttctatgag
    19741 ccggaaagct ccagcgcgtt gggctttggt ttccgctgcg gcttccttgg cctgctgcac
    19801 atggagatca tccaggaacg tctggaacgt gaatacgatc tggatctgat caccactgcg
    19861 ccgactgtag tgtatgaagt tgaaaccacg tcaagagaag ttatctacgt cgacagccca
    19921 tccaagctgc cagcggtaaa taacatttac gaactgcgcg agccgattgc agagtgtcac
    19981 atgctgctgc cgcaggcata tctcggcaac gttattacgc tgtgcgtaga aaaacgcggc
    20041 gtgcagacca atatggttta ccacggtaat caggtggcgc tgacgtacga gatcccgatg
    20101 gcggaagtgg tgctcgactt cttcgatcgc ctgaaatcta cctcgcgtgg ttatgcgtct
    20161 ctggattaca acttcaaacg cttccaggcg tccgacatgg tacgtgtaga cgtattaatc
    20221 aacggtgaac gtgttgatgc attggcgctg atcacccacc gtggtaattc gcaaaaccgc
    20281 ggtcgcgagc tggtggagaa gatgaaagat ctgatcccac gccagcagtt tgatatcgcc
    20341 attcaggcag cgattggtac gcacatcatt gcgcgctcca ccgtgaaaca gctgcgtaaa
    20401 aacgtactgg ctaaatgtta tggcggcgat atcagccgta agaaaaagct gctgcagaag
    20461 cagaaagaag gtaagaaacg catgaagcag atcggtaacg tcgagctgcc gcaggaagcg
    20521 ttcctcgcca ttctgcacgt cggcaaagac aacaaataac ccttaggagt tggcatggcg
    20581 aatatgtttg ccctgattct ggtgattgcc acactggtga cgggcatttt atggtgcgtg
    20641 gataaattct ttttcgcacc taaacggcgg gaacgtcagg cagcggcgca ggcggctgcc
    20701 ggtgactcac tggataaagc aacgctgaaa aaggttgcgc cgaagcctgg ctggctggaa
    20761 accggtgctt ctgtttttcc ggtactggct atcgtattga ttgtgcgttc gtttatttat
    20821 gaaccgttcc agatcccgtc aggttcgatg atgccgactc tgttaatcgg tgattttatt
    20881 ctggtagaga agtttgctta tggcattaaa gatcctatct accagaaaac gctgatcgaa
    20941 accggtcatc cgaaacgcgg cgatatcgtg gtctttaaat acccggaaga tccaaagctt
    21001 gattacatca agcgcgcggt gggtttaccg ggcgataaag tcacttatga tccggtctca
    21061 aaagagctga cgattcaacc gggatgcagt tccggccagg cgtgtgaaaa cgcgctaccg
    21121 gtcacttact ccaacgtgga accgagcgat ttcgttcaga ccttctcacg ccgtaatggt
    21181 ggggaagcga ccagcggatt ctttgaagtg ccgaaaaacg aaaccaaaga aaatggaatt
    21241 cgtctttccg agcgtaaaga gacactgggt gatgtgacgc accgtattct gacagtgccg
    21301 attgcgcagg accaggtggg gatgtattac cagcagccag ggcaacaact ggcaacctgg
    21361 attgttccgc cgggacaata cttcatgatg ggcgacaacc gcgacaacag cgcggacagt
    21421 cgttactggg gctttgtgcc tgaagcgaat ctggtcggtc gggcaacggc tatctggatg
    21481 agcttcgata agcaagaagg cgaatggccg acaggtgtgc gcttaagtcg cattggcggt
    21541 atccattaat agccatcttc gttcacgttg tcgccgttat ggcgacaacg tgaattattt
    21601 atgagataaa tctcccgtgg ctaacgacat cccccgtcgt tgtgtataga atattccccc
    21661 gaagtttaag gttggcacct ccaggttgcc acggcacacg aaacagcgtt ggtccccata
    21721 taccggtaaa ctgaaactgc agtgaagcag ttagccgagc catgtatatc aggtctgttt
    21781 cgtgtgctga attgttgacg catttattta ttggtatcgc atgaacccca tcgtaattaa
    21841 tcggcttcaa cggaagctgg gctacacttt taatcatcag gaactgttgc agcaggcatt
    21901 aactcatcgt agtgccagca gtaaacataa cgagcgtctg gaatttttag gtgactctat
    21961 tctgagctac gttatcgcca atgcgcttta tcaccgtttc cctcgtgtgg atgaaggcga
    22021 tatgagccgg atgcgcgcca cgctggtccg tggtaatacg ctggctgaac tggcgcgcga
    22081 atttgagtta ggtgagtgct tacgtttagg gccaggtgaa cttaaaagcg gtggatttcg
    22141 tcgtgagtca attctcgccg acaccgtcga agcattaatt ggtggcgtat tcctcgacag
    22201 tgatattcaa accgtcgaga aattaatcct caactggtat caaactcgtc tggacgaaat
    22261 tagcccaggc gataaacaaa aagatccgaa aacgcgtctg caggaatatt tgcagggtcg
    22321 ccatctgccg ctgccaactt atctggtagt ccaggtacgt ggcgaagcgc acgatcagga
    22381 atttactatc cactgccagg tcagcggcct gagtgaaccg gtggttggca caggttcaag
    22441 ccgtcgtaag gctgagcagg ctgccgccga acaggcgttg aaaaaactgg agctggaatg
    22501 agcatcgata aaagttactg cggatttatt gctatcgtcg gacgtccgaa cgttggcaaa
    22561 tccacattgt tgaacaaact gctggggcag aagatctcca ttacctcccg caaggcgcaa
    22621 acaactcgtc accgcattgt ggggatccat actgaaggcg cgtatcaggc gatctacgtt
    22681 gataccccag gcctgcatat ggaagaaaaa cgcgccatta accgtctgat gaacaaagcg
    22741 gcgagcagct ccattggcga tgttgagctg gtgatttttg tcgtagaagg cactcgctgg
    22801 acgccggacg atgaaatggt gctcaacaaa ctgcgcgatg gcaaagcgcc ggtaatcctc
    22861 gcggtgaaca aagtggataa tgtgcaggaa aaagccgatc tgctgccaca cttacagttc
    22921 ctggcaagcc agatgaactt cctcgatatc gtgccgatct ctgccgaaac cgggctgaat
    22981 gtcgacacta ttgcagcaat cgtgcgtaag catctgcctg aagcgaccca tcacttcccg
    23041 gaagattaca tcaccgatcg ctcacagcgt tttatggcgt ctgaaattat ccgcgaaaaa
    23101 ctgatgcgtt ttctcggcgc tgaactgccg tactccgtga ccgtggagat cgaacgtttc
    23161 gtctctaacg aacgcggcgg ttatgacatc aacggtttga ttctcgttga gcgtgaaggg
    23221 cagaagaaga tggtcattgg caacaaaggg gccaagatca aaaccatcgg gattgaagcg
    23281 cgtaaagaca tgcaggaaat gttcgaagcg cctgttcacc ttgagctgtg ggtaaaagtg
    23341 aaatccggtt gggccgacga cgaacgcgca ctgcgcagtc tcggttatgt tgacgatctt
    23401 taagagtaac tccgatggaa ggctggcagc gcgcatttgt cctgcatagt cgcccgtgga
    23461 gcgaaaccag cctgatgctg gacgtcttca cggaggaatc gggtcgcgtg cgtctggttg
    23521 ccaaaggcgc acgctctaaa cgctctactc tgaaaggtgc attacagcct ttcacccctc
    23581 tcttgctacg ttttggcggg cgtggcgaag tcaaaacgct gcgcagtgct gaagctgtct
    23641 cgctggcgct gccattaagc ggtatcacgc tttacagcgg tctgtacatc aacgaacttc
    23701 tctcccgcgt actggaatac gagacgcgct tctctgaact ctttttcgat tacttgcact
    23761 gcattcagtc tcttgccggg gtcactggta cgccagaacc cgcgttgcgc cgctttgaac
    23821 tggcactgct cgggcatctg ggctatggcg tcaattttac ccattgtgcg ggtagcggcg
    23881 agccggtaga tgacaccatg acgtatcgtt atcgcgaaga aaaagggttt atcgcaagcg
    23941 tcgttatcga caataaaacg ttcaccggaa ggcagttaaa agcgttaaac gcgcgggaat
    24001 ttcctgacgc agacacactg cgcgccgcga aacgctttac ccgcatggcg cttaagccgt
    24061 atcttggcgg taaaccatta aagagcaggg aactgttccg gcagtttatg cctaagcgaa
    24121 cggtgaaaac acattatgaa tgatgaggat tgtcatggct gaattactgt taggcgtcaa
    24181 cattgaccat attgcaacac tgcgtaacgc gcgcggtact gcttacccgg atccggtgca
    24241 ggcggcgttt attgctgagc aggcgggagc ggacggcatt accgtgcatt tacgtgaaga
    24301 ccgccgccac attaccgacc gcgatgtgtg catcctgcgc cagacgctgg atacccgcat
    24361 gaatctggag atggcggtga ccgaagagat gctggcgatc gccgttgaga cgaagccaca
    24421 tttttgctgt ctggtaccgg aaaagcgtca ggaagtaaca accgaaggcg gcctggacgt
    24481 cgcagggcag cgtgaaaaaa tgcgcgatgc ctgcaaacgt ctggcagatg ccgggatcca
    24541 ggtttctctg tttatcgacg ccgatgaaga gcagatcaaa gctgcggcag aggttggcgc
    24601 accgtttatc gagatccaca ccggttgcta tgctgatgcc aaaactgacg ccgaacaggc
    24661 gcaagagttg gcgcgtatcg ccaaagccgc gacctttgcc actagtctcg gtctgaaagt
    24721 taacgccgga cacggtctga cctatcacaa cgtgaaagcc attgccgcca tccctgagat
    24781 gcatgaactg aatatcggtc atgccattat tggtcgtgca gtgatgaccg gactgaaaga
    24841 tgcggtggca gaaatgaagc gtctgatgct ggaagcgcgt ggctaatggc aatattaggt
    24901 ttaggtacgg atatcgtaga gatcgctcgc atagaagcgg tgatcgcccg atccggtgag
    24961 cgcctggcac gccgcgtatt aagcgataac gaatgggaaa tctggaaaac gcaccaccag
    25021 ccagtgcgtt ttctggcgaa gcgttttgct gtgaaagaag cggcggcaaa agcatttggc
    25081 acagggatcc gcaatggtct ggcgtttaat cagtttgaag tattcaatga tgagctcggc
    25141 aaaccacggc tacggctatg gggtgaggca ttaaaactgg cggaaaagct cggcgttgta
    25201 aatatgcatg taac
//
LOCUS       DAHHTG010000066        24816 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.066, whole genome shotgun sequence.
ACCESSION   DAHHTG010000066 DAHHTG010000000
VERSION     DAHHTG010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 24816)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24816)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24816
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.066"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            445..1641
                     /locus_tag="NBI74_002621"
     CDS             445..1641
                     /locus_tag="NBI74_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110-like element ISEc20 family transposase"
                     /protein_id="HCD1932937.1"
                     /translation="MTESSDYESVQVFIGVDVGKDTHHAVAINRSGKRLFDKALPNDE
                     NKLRSLISDLKQHGQILLVVDQPATIGALPVAVARSEGVLVGYLPGLAMRRIADLHAG
                     EAKTDARDAAIIAEAARTLPHALRTLKLADEQIAELSMLCGFDDDLAAQTTQASNRIR
                     GLLTQIHPALERVLGPRLDHPAVLDLLQRYPSPEKLASLGEKKLAAQLCKLAPRLGKR
                     LAADIAQALAEQTVVVPGTNAAAVVLPRLALQLITLRKQRDEVALAVEQRVLAHPLYP
                     VLTSMPGVGVRTAARLLTEVACRAFASVAHLAAYAGLAPVTRRSGSSIRGEHPSRRGN
                     KALKRALFLSAFAALRDPLSRAYYTRKMSQGKRHNQALIALARRRCDVLFAMMHDETF
                     YTPQAS"
     gene            1837..2043
                     /locus_tag="NBI74_002622"
     CDS             1837..2043
                     /locus_tag="NBI74_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020234041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family phage regulatory protein"
                     /protein_id="HCD1932938.1"
                     /translation="MATPVSLMDDQMVDMAFITQLTGLTDKWFYKLIKVGGFPAPIKM
                     GRSSRWLKSEVEAWLQARIAQSRP"
     gene            2137..2739
                     /locus_tag="NBI74_002623"
     CDS             2137..2739
                     /locus_tag="NBI74_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000813428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1932939.1"
                     /translation="MKQQYQTRYELLHENYQKWLTGFTRHAVSWGVCHPNIYYFHNLT
                     PGWVSFNGEKPEIAIVPQSLHRLIYGPDKRSSPSLDDDLVVNLCTSEHLLVHHPMLEG
                     ILLSECERLKQHSLANKLISLFRQFGGTELRLKLVWLCWLDLMTGNSLDDWTKNLKHK
                     SEKDLEQWIIARQGQSEPLTNLMDQYVLMAYRTSVDAAHS"
     gene            3213..3428
                     /locus_tag="NBI74_002624"
     CDS             3213..3428
                     /locus_tag="NBI74_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001331140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1932940.1"
                     /translation="MAWNTDVSADGKPHSLWKQGRALASQPESVTKTNIAENRIHRSG
                     VNARRQKPLSAQPPSDLNKKHTHNRLT"
     gene            3570..3698
                     /locus_tag="NBI74_002625"
                     /pseudo
     CDS             3570..3698
                     /locus_tag="NBI74_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000952428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
     gene            complement(3765..4548)
                     /gene="istB"
                     /locus_tag="NBI74_002626"
                     /pseudo
     CDS             complement(3765..4548)
                     /gene="istB"
                     /locus_tag="NBI74_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858162.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element IS100kyp family helper ATPase
                     IstB"
     gene            complement(4545..5567)
                     /gene="istA"
                     /locus_tag="NBI74_002627"
     CDS             complement(4545..5567)
                     /gene="istA"
                     /locus_tag="NBI74_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HCD1932941.1"
                     /translation="MVTFETVMEIKILHKQGMSSRAIARELGISRNTVKRYLQAKSEP
                     PKYTPRPAVASLLDEYRDYIRQRIADAHPYKIPATVIAREIRDQGYRGGMTILRGFIR
                     SLSVPQEQEPAVRFETEPGRQMQVDWGTMRNGRSPLHVFVAVLGYSRMLYIEFTDNMR
                     YDTLETCHRNAFRFFGGVPREVLYDNMKTVVLQRDAYQTGQHRFHPSLWQFGKEMGFS
                     PRLCRPFRAQTKGKVERMVQYTRNSFYIPLMTRLRPMGITVDVETANRHGLRWLHDVA
                     NQRKHETIQARPCDRWLEEQQSMLALPPEKKEYDVHPGENLVNFDKPPLHHPLSIYDS
                     FCRGVA"
     gene            5656..5766
                     /locus_tag="NBI74_002628"
                     /pseudo
     CDS             5656..5766
                     /locus_tag="NBI74_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000952428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
     gene            5810..5965
                     /locus_tag="NBI74_002629"
                     /pseudo
     CDS             5810..5965
                     /locus_tag="NBI74_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000544830.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
     gene            7065..8237
                     /locus_tag="NBI74_002630"
     CDS             7065..8237
                     /locus_tag="NBI74_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000263646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1932942.1"
                     /translation="MWEEQVRCIVGKELDDICTSCPYIDAIKRHKQQLGAIEEYTQWL
                     KKEPRASYFFLFRLYTRIHNTFFPKKQQLPFTPGGTHCPEPDVTLRDLTLSPGYHSDY
                     APQPIPEMDSSAVVPPTNENTSPPEDTPDNTPAGGNTGQAEKTRNSGLTPIPEKRSGM
                     PPEHLRFATGFPPQPKIAGPKGKPMRTVHPDKIYREIIWFCSGYLRKSGPEATRTIIN
                     SIFCEWASIFNDYSSPFSWVDSRDSEQCDWLWNAMQVRCVGTPLNPLTPEQKYWFACA
                     TFDNWEGWNEQQVQFLLESNPRRNRAKFTQASFQAPRIQHKAILLDELKSAREQQKRR
                     DERADGSVPLKLSGKIHKQLESIARSRGVLPKKLLNEMIEQAYQDFVANEQHKTLS"
     gene            complement(8768..9025)
                     /locus_tag="NBI74_002631"
     CDS             complement(8768..9025)
                     /locus_tag="NBI74_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001163787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1932943.1"
                     /translation="MQHIDRLNVIKALVLLEDEQIVRFNIAANDNASQIHMLVDGLGV
                     GLTHEPVAAGMISHRTACHRSRQHDRPAHSYFYYLSAYQVA"
     gene            complement(9079..9846)
                     /locus_tag="NBI74_002632"
     CDS             complement(9079..9846)
                     /locus_tag="NBI74_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001608034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCD1932944.1"
                     /translation="MSGLTINALCAGYGKRRIIEHLSISTLPRGEVTVLLGPNGCGKS
                     TLLRALAGLNRASGEAWLNEENLLSLPFARRAEKVVFLPQSLPQGVHLQVLESVVVAQ
                     RASGSGQNQAQAIALLEELGIAHLAMNYLDSLSGGQKQLVGLAQSLIRRPALLLLDEP
                     LSALDLNYQFHVMDVVSRETRRRNMVTLVVLHDINIALRHAAQVIMLKEGKLIDSGDP
                     QTVIHAESLAQVYGVRGRVERCAQGRSMVIVDGAIEK"
     gene            complement(9843..10901)
                     /locus_tag="NBI74_002633"
     CDS             complement(9843..10901)
                     /locus_tag="NBI74_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001546670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HCD1932945.1"
                     /translation="MTVLESTLSVENRYRQLFRQRLVILAVIFIAIIASLLLDFTLGP
                     SGLPLHSLIKTLMHPSGATNGMRVIVWDIRLPYALMALLVGMALGLAGAEMQTILNNS
                     LASPFTLGVSSAAAFGAALAIVLGIGIPGVPESGFIPANAFLFALLSALLLDSLTRWT
                     RVPTSGVVLFGIALVFTFNALVSIMQFVADEDTLQGLVFWTMGSLARASWEKLAVLAA
                     AMAIVLPWSLRRAWQLTALRLGEERAMSFGIDVRRLRLGSLLRISLLAALSVAFVGPI
                     GFIGLVAPHISRLLLGEDHRFYLPGSILIGGLVLSLASVASKNIIPGVILPVGIVTSL
                     VGVPFFLSIVMRHRGSMS"
     gene            complement(10920..11909)
                     /locus_tag="NBI74_002634"
     CDS             complement(10920..11909)
                     /locus_tag="NBI74_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000778018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCD1932946.1"
                     /translation="MKLSRLFPLLALLLAASLHAENTHKEVRIASPWPAQNTIIAMLG
                     YGDSIVGTSMIAKRIPLFRQSLPRIEKVAAVSVNSGHEINPEQIIALGVDMLFVPQNM
                     VVPQQALLKQAGVQVLAFEANSLRALTQRVQQTAAVLGPDAQQKALAYQRYFDRNVAL
                     VTGRLKDLPASQRVSLYHSMGNPLTTTGRPSLNQDWIDLAGGKNIAENWFGEHQQNRS
                     GEVALEKIVTANPAVIIAMNKRDADAILSSPQWASVDAVIHHRVYVNPKGMFWWCRET
                     SEEALQFLWLAKTLYPARFADVDIRKETREFYRQFFGLTLSDAQMSDVLNPPR"
     gene            complement(11920..14085)
                     /locus_tag="NBI74_002635"
     CDS             complement(11920..14085)
                     /locus_tag="NBI74_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000856948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HCD1932947.1"
                     /translation="MKTQITFAALLPALASFIPLHAHASSTSEDEMIVTGNTAADTTD
                     SAAGAGFKTNDIDVGPLGTKSWIETPYSSTTVTKEMIENQQAQSVSEMLKYSPSTQMQ
                     ARGGMDVGRPQSRGMQGSVVANSRLDGLNIVSTTAFPVEMLERMDVLNSLTGALYGPA
                     SPAGQFNFVAKRPTEETLRKVTLGYQSRSAFTGHADLGGHFDENKRFGYRVNLLDQEG
                     EGNVDDSTLRRKLVSVALDWNIQPGTQLQLDASHYEFIQKGYVGSFNYGPNVKLPSAP
                     NPKDKNLALSTAGNDLTTDTISTRLIHYFNDDWSMNAGVGWQQADRAMRSVSSKILNN
                     QGDISRSMKDSTAAGRFRVLSNTAGLNGHIDTGSIGHDLSLSTTGYVWSLYSAKGTGS
                     SYSWGTTNMYHPDAIDEQGDGKIRTGGPRYRSSVNTQQSVTLGDTVTFTPQWSAMFYL
                     SQSWLQTKNYDKHGNQTNQVDENGLSPNAALMYKITPNTMAYVSYADSLEQGGTAPTD
                     ESVKNAGQTLNPYRSKQYEVGLKSDIGEMNLGAALFRLERPFAYLDTDNVYKEQGNQV
                     NNGLELTAAGNVWQGLNIYSGVTFLDPKLKDTANASTSNKQVVGVPKVQANLLAEYSL
                     PSIPEWVYSANVHYTGKRAANDTNTSYASSYTTWDLGTRYTTKVSNVPTTFRVVVNNV
                     FDKHYWASIFPSGTDGDNGSPSAFIGGGREVRASVTFDF"
     gene            14514..14948
                     /locus_tag="NBI74_002636"
     CDS             14514..14948
                     /locus_tag="NBI74_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1932948.1"
                     /translation="MNPSDAIEAIEKPLSSLPYPISRHILEHLRKLTRHEPVPGIMGK
                     SGAGKSSLCNALFQGEVTPVSALMTVLPGHAAIHLMTRLQDELRTESVRTQTREQFTG
                     AVDRIFDTAESVCIASVARTVLRAVRDSVVSVARAVWNWIFF"
     gene            15166..17550
                     /locus_tag="NBI74_002637"
     CDS             15166..17550
                     /locus_tag="NBI74_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dynamin family protein"
                     /protein_id="HCD1932949.1"
                     /translation="MHEKNIALLCDEADRLLQLNINLLRQMVEEPDVLSDSKNENRLL
                     FDKQKALKRIEELEGEQIKTARREMVLAVVGTMKAGKSTTINAIVGQEILPNRNRPMT
                     SVPTLIRHVPGKTEPVLHLEHIQPVRNLLITLQEKLATPAGQQVAQTLQQTGDTRELL
                     DILTDDGWLKNEYHGEEEIFTGLASLNDLVRLAAAMGTEFPFDEYAEVQKLPVIDVEF
                     SHLVGMDACQGTLTLLDTPGPNEAGQPQMEVMMRDQLQKASAVLAVMDYTQMNSKADE
                     DVRKELNAIADVSAGRLFVLVNKFDEKDRNGDGADAVRQKVPAMLNSDVLPASRVYPG
                     SSRQAYLANRALHELRKNGTLPVDEAWVDDFVREAFGRMKKDYVCKDSELATEGATDL
                     WECSLIDQLITEVILSSHSRAAALAVDSAAAKLMQNAENISEYLSLRHQGLMQSIQSL
                     QAHITSLLEDIREIAGCQEQVTADVRMAMEEIDARTRELLTGVCTSLEEELNDYFRSG
                     KRKEQQMLEEENSAQPRERNAFAFFHDIFGTGNQHDRMRDFDPDSPEIKFSDRREALE
                     LMTQIESTVTSLHREAEAQFRPELEKIVSGIETGFRGTALYATENIAGRINTRLEDEG
                     FTVKISFPAVSQLQTRLAVKTNLSALMEERTETVTRRRRQSGLWGKICGAFGTSDWGW
                     ETYKEDVSRSVININTVRKEVMSLTRAYFGELQASIEQDINQPVRQEIDAFFCAFREK
                     VEQLRNTLIQSAEDHKRDQQAQERLTRRLQALNERVPELITDSKALREELETML"
     gene            17547..18452
                     /locus_tag="NBI74_002638"
     CDS             17547..18452
                     /locus_tag="NBI74_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein"
                     /protein_id="HCD1932950.1"
                     /translation="MTSPFIQQIADNRVCQVLTCLPEKFVVDFANGIDVAQEHIRTAG
                     ERTFFRRLKEGLTGEGAARQNAINASVAQGLEASLRWLTELTTSLATTNYAITRVNDR
                     VSSLVSDTARLAHYSADTREQLLILADQVHHKLNHLEEKLHRVDQVQRAQLHLEQIFS
                     WWSAGRYASFSPAGRCYVALEELRWGAFGDVIRQGETGQVNQLLDILRHKALTQMAQE
                     SGGSATVRLNTLDWLGGQGREQADNEWHDAINWLGDWCSEEQHPVIWSTTQAAEHLPV
                     RMPRLCSAERLSESMVDEIFQKGAA"
     gene            18449..19519
                     /locus_tag="NBI74_002639"
     CDS             18449..19519
                     /locus_tag="NBI74_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HCD1932951.1"
                     /translation="MSTEMKTGLVLSGGGAVGAYQAGVVKALAECGTQISMVSGASIG
                     AFNGAIIAASPDLSEAAVRLEALWDHLGNNQVLSVNRLVYFSLLKKLFQAMNLCQIPG
                     RAGALLTTLLRHISILNGFDNLMAQPLLSDEPLTALLDHYLDTDALADGLPLYVSLYP
                     TEGGMQDIIDCIRAELGAGTTKNAVFQHIQSLPRGQQKEALLASAALPLLFRPREVQG
                     TMFGDGGMGGWRNMQGNTPVTPLVDAGCNMVIVTHLSDGSLWDRQAFPDTTILEIRPR
                     KRLKYAGDGDNSGGLLSFTLAHTDAWRQQGYEDTMLTMEHIRKPLAARQVLSRSETVL
                     QKSLEITEEADLALRNAMARIK"
     gene            19655..20332
                     /locus_tag="NBI74_002640"
     CDS             19655..20332
                     /locus_tag="NBI74_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1932952.1"
                     /translation="MQLPVKLLMSLISLVSVIARAGKYKNYIRDEIKYWRYTSYKGGE
                     FPEGFTDEKFSSAIYNGRIFTMKRLHTLMLFLAVLFTGFNVEAASVKQALSCDPNARA
                     EQPGACPTTYELYEGDAAYKAALDKALKPVGLSGMFGKGGYMDGPGGNVTPVTINGTV
                     WLQGDGCKANTCGWDFIVTLYNPKTHEVVGYRYFGLDDPAYLVWFGEIGVHEFAYLVK
                     NYVAAVN"
     gene            20348..20758
                     /locus_tag="NBI74_002641"
     CDS             20348..20758
                     /locus_tag="NBI74_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000846703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1932953.1"
                     /translation="MKTFIKTLLVAVTILFSVFATAKQVKLPNNIKYVNTTEAFSCTE
                     IDGMNCQTKNPFNYKDNSYVFVLERGGAWCYDYTVSVLNLKTGKAQMLEYKDNQLCSG
                     SNKPFFEIKNGVPTVGVIDTSGKPVVVALDKLKT"
     gene            20979..21800
                     /locus_tag="NBI74_002642"
     CDS             20979..21800
                     /locus_tag="NBI74_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF945 domain-containing protein"
                     /protein_id="HCD1932954.1"
                     /translation="MRLASRFGYTANQIRRDRPLTHEELMHHVPGIFGEEKHTSRSQN
                     YTYIPTITVLESLQREGFQPFFACQTRVRDPGRRGYTKHMLRLRRAGEINGEHVPEII
                     LLNSHDGTSSYQMLPGYFRFVCQNGCVCGQSLGEVRVPHRGDVVEKVIEGAYEVVGVF
                     DRIEEKRDAMQSLVLPPPARQALAQAALTYRYGDEHQPVTTADILTPRRREDYGKDLW
                     SAYQTIQENMLKGGISGRSAKGKRIHTRAIHSIDTDIKLNRALWVMAETMLESLR"
     gene            22063..22536
                     /locus_tag="NBI74_002643"
     CDS             22063..22536
                     /locus_tag="NBI74_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708769.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HCD1932955.1"
                     /translation="MKTLSQNTTSSACAPETGLQQLVATIVPDEQRISFWPQHFGLIP
                     QWVTLEPRVFGWMDRLCEDYCGGIWNLYTLNNGGAFMAPEPDDDDDETWVLFNAMNGN
                     RAEMSPEAAGIAACLMAYSHHACRTENYAMTVHYYRLRDYALQHPECSAIMRIID"
     gene            22552..23028
                     /locus_tag="NBI74_002644"
     CDS             22552..23028
                     /locus_tag="NBI74_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RadC family protein"
                     /protein_id="HCD1932956.1"
                     /translation="MQQLSFLPGEMTPGERSLIQRALKTLDRHLHEPGVAFTSTRAAR
                     EWLILNMAGLEREEFRVLYLNNQNQLIAGETLFTGTINRTEVHPREVIKRALYHNAAA
                     VVLAHNHPSGEVTPSKADRLITERLVQALGLVDIRVPDHLIVGGNQVFSFAEHGLL"
     gene            23091..23312
                     /locus_tag="NBI74_002645"
     CDS             23091..23312
                     /locus_tag="NBI74_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 domain-containing protein"
                     /protein_id="HCD1932957.1"
                     /translation="MKIITRGEAMRIHQQHPTSRLFPFCTGKYRWHGSAEAYTGREVQ
                     DIPGVLAVFAERRKDSFGPYVRLMSVTLN"
     gene            23392..23760
                     /gene="cbtA"
                     /locus_tag="NBI74_002646"
     CDS             23392..23760
                     /gene="cbtA"
                     /locus_tag="NBI74_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system toxin CbtA"
                     /protein_id="HCD1932958.1"
                     /translation="MSDTLPGTTLPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYL
                     ADRASIRGLFSDADAYHPDQAFPLLMKQLELMLTSGELNPRHQHTVTLYAKGLTCKAD
                     TLSSCGYVYLAVYPTPEMKN"
     gene            23849..24223
                     /gene="cbtA"
                     /locus_tag="NBI74_002647"
     CDS             23849..24223
                     /gene="cbtA"
                     /locus_tag="NBI74_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system toxin CbtA"
                     /protein_id="HCD1932959.1"
                     /translation="MKTLPVLPGQAASSRPSPVEIWQILLSRLLDQHYGLTLNDTPFA
                     DERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGFSACTRSQLINSIDILRARRATG
                     LMTRDNYRTVNNITLGKYPEAK"
     gene            24220..24414
                     /locus_tag="NBI74_002648"
     CDS             24220..24414
                     /locus_tag="NBI74_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310834.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1932960.1"
                     /translation="MMTLEADSVNVQALDMGHIVVDIDGVNITELINKAAENGYSLRV
                     VDGRDSTETPATYASPHQLL"
     gene            24427..24540
                     /locus_tag="NBI74_002649"
     CDS             24427..24540
                     /locus_tag="NBI74_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001161660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF957 domain-containing protein"
                     /protein_id="HCD1932961.1"
                     /translation="MQGIVPLTTETALLSCIKQAREDVLTLRHLQLLHQNR"
BASE COUNT         6183 a         6566 c         6387 g         5680 t
ORIGIN      
        1 gttactctct gatgacttct tatatttata ataccaatca gacaacctta tcccatcggt
       61 gatatcagtc tcctgctcgc caggaacggg ctgactcact gtaaccacct ccgaaaacaa
      121 ctctgcaatt atcagaacgc ctgcttctct ccctgtcctc acgaaaacta tcccctcttt
      181 atcgcgcgtg cgtgcggaag catcttttcg cacccccgtt actttattcg taccccttat
      241 aatggggtgt tagccagcca gacccggcat gattactgcc cccagtcgtc catgatccgg
      301 ggggtgatgt caccgggtct ggtggggcgc tggtaaccgc taataggggt caggtcaggc
      361 acttttgccg ggaccgtctg taacgtggat gccggtacct gctccccgtg gttatctggt
      421 taacccatat acaagggaga cagaatgacc gaatccagcg attacgaatc cgtccaggtc
      481 tttatcggcg ttgatgtcgg taaagatacg catcacgctg tagccattaa tcgttcaggt
      541 aaacgcctgt tcgataaagc attacccaac gacgaaaaca aactcaggtc gctaatatct
      601 gacctgaaac aacatggtca gatactgctg gttgttgatc agccagctac catcggtgcg
      661 ttacctgtcg ccgttgcccg ctcagaagga gtccttgtcg gatacctccc tggactggcc
      721 atgcgccgca tagccgactt acacgccggt gaagctaaaa ctgatgctcg tgacgctgcc
      781 atcattgccg aagctgcccg taccctgcct cacgcgctac gcacgctgaa actggctgac
      841 gagcaaatcg ccgaactctc catgctctgc ggcttcgatg atgatcttgc cgcacagaca
      901 acgcaggcca gcaaccgtat ccgcggcctt ctgacccaga tacatccggc actggagcgc
      961 gttctcggtc cgagacttga tcacccggcg gtactcgatc ttctccagcg atatccctca
     1021 ccagaaaaac tcgcttcgct gggtgagaag aagctggcag cccagctctg caaacttgcg
     1081 cctcgtctgg gtaaacgcct tgcagcagac atagctcagg cactggccga acaaaccgtc
     1141 gtcgttcccg gcacgaatgc cgctgccgta gtactgccac gtctggcact ccagctcatc
     1201 acgctgcgta agcaaagaga cgaggtggcg cttgcggtag aacagcgagt tcttgctcac
     1261 cctctttacc cggtcctgac cagtatgccc ggagtcggtg tcaggaccgc agccagactc
     1321 ctcaccgagg tcgcctgccg cgccttcgcc tctgtcgcac atctcgctgc ttatgctggc
     1381 cttgcgccgg taactcggcg atccggctcg tcaatacgcg gtgagcatcc ctcgcgacgg
     1441 ggtaataaag ctctcaaacg ggcgttgttc ctgtcggcct tcgccgcgct cagggatccg
     1501 ctctccaggg cttactacac ccgcaaaatg agtcagggaa aacgacacaa tcaggcgctt
     1561 atcgccctgg cgagacgacg ctgcgacgtt ctgttcgcca tgatgcacga cgagactttt
     1621 tataccccgc aggcgtcata acatgcttga caacttaata ggggcacccc ccgggattcc
     1681 gctacggctc tgccatcgca atccccccgt ttatctccgg acagccagat tcccgattat
     1741 tttttacgtt tctccccggt tgttatgccg gtgaaggtgg cgcgctgttt tcatgaccac
     1801 accggctggt ttaacaacat ccggaggaac attctcatgg ctaccccagt ttcgctgatg
     1861 gatgaccaga tggtcgacat ggcgtttatc actcaactga ccggcttaac cgataagtgg
     1921 ttttacaaac tcatcaaagt tgggggcttt cctgccccca tcaaaatggg gcgcagctcc
     1981 cgctggctga aaagtgaagt ggaagcctgg ctgcaggcgc gtattgcaca gtcccgtccg
     2041 taatttttaa ccactaaccc ttcgcccaaa gcagacgcaa tctccggcct gccggcggcg
     2101 ctctgctgcc tgttatcccc cgtgaggaat atgaaaatga aacaacagta tcagacccgt
     2161 tacgagctgc tccatgaaaa ctaccagaaa tggctgaccg gcttcacccg gcacgccgta
     2221 tcatggggcg tgtgtcatcc gaatatctac tatttccata atctgacgcc cgggtgggtg
     2281 tcattcaatg gcgaaaaacc ggagattgcc atcgttcccc agagtctgca ccggctgatt
     2341 tatggtccgg acaaacggag ctccccgtcc ctggatgatg atctggtggt gaatttatgc
     2401 accagcgaac acctgctggt tcatcatccg atgctggaag ggatcctgct gtctgaatgt
     2461 gagcgcctga agcaacattc tctggcaaac aaactgatca gcctcttccg tcagtttggc
     2521 ggcacggagt tacgtctcaa actggtctgg ctctgctggc ttgacttaat gaccggaaac
     2581 agccttgacg actggacgaa aaacctgaaa cacaaatcag aaaaagatct ggagcaatgg
     2641 atcattgcac gtcagggtca gagtgaacca ctgacaaatc tgatggatca gtacgtgctt
     2701 atggcatacc gtacatcggt tgatgccgct cacagttgat gtctgataac cacagggctg
     2761 gcagcagaaa accagccctg tggtaaaata cagtaatagc ggcgtacatc aggcacgccg
     2821 ctcactcgtc cacagatcat atacgtcatt atactgcgcg ataacggagg gcttcagcac
     2881 ccggtcattt ctgagcgggt atgaatacca ttgatgatgc gaagcagcca cgggcagaaa
     2941 cgttcttcac aaccaggtgc ctccatcttc actgcaggct gaatgtgccg gcttgttatc
     3001 caccagaaat ggtatgctcc ggtacatgca taccccggag actataaacg cactgaatca
     3061 tagcctccca cctcagcagg aggcagtcag ctttcaggcg gaaaaacgtt gcagttaaag
     3121 atcgtgagaa tcgctgtttc gttgcatagc aacccgaaca gttgtagttg aaacatttta
     3181 cctgcatcag cacatggtca ggcgccgtct gtatggcgtg gaatactgat gtttcggctg
     3241 atggcaagcc tcattctctg tggaaacagg ggcgcgcatt agcttctcaa ccagagagtg
     3301 tcacaaagac aaatattgct gaaaatcgca ttcatcgttc aggcgtgaat gcccggagac
     3361 aaaagccgct gtccgcacag cccccctctg atctgaacaa aaagcataca cacaacaggc
     3421 tcacctgacc ccgaaaaacc ataacttcag caataccagt gacaccagca cgttacctca
     3481 gtgccacatc ttcagaccgc gatcaatctg aaatgtgact atcggggcaa tatcccagtt
     3541 ttgtggtaca cgataaccag aaaacatccc ctgaaccgcc agccagaaag accggccaca
     3601 aaagggttaa gctgaaatcg tttatggatt acatcgacat atgcctggca gagaatgtcc
     3661 ggaatgacga ggttatcttt gcggagatta aggcaatgtg tcaacgacgg atgaaaagtg
     3721 atccacttat atctccacca acggcccaat attgatccac cgttttactc aggattagct
     3781 tctgctataa ccccggcttt tcgtttctgt ctgagtcgat agctttctcc tttgatttga
     3841 acgacatgtg agtggtgtaa gatacggtcc agcatcgctg aggtcagtgc tgcatcaccg
     3901 gcgaacgttt gatcccactg cccgaacggc agattggatg tcaggatcat tgcgctcttt
     3961 tcgtaacgtt tagcaatgac ctggaagaac agttttgctt cttcctgact gaacggcaga
     4021 tagcctattt catcaatgat gagcaggcgg ggggccatta ctccacgctg aagcgtcgtt
     4081 ttataacggc cctgacgttg tgccgtagat aactgaagta acagatctgc tgctgttgtg
     4141 aagcgaactt tgatacctgc acggacggct tcatagccca tcgctattgc cagatgggtt
     4201 ttccccacac ctgatggccc cagtaatacg atattttcat tacgttctat gaagctgagt
     4261 gagcgtaacg actggagttg cttctgcggt gctccggtgg cgaatgtgaa gtcatactct
     4321 tcgaacgttt tcaccgccgg gaaggctgcc attcgggtat acatcgcctg tttacgttga
     4381 tgacgtgcca gtttttcttc atgaagcatg atgctccagg aagtccatat aactccattc
     4441 ctggtctact gcctgttgtg acagcgcagg cgctgcgctt ataaggcttt ccagttgcaa
     4501 ctgcccggcg agcgccatca gtcgttgatg ttgcagttcc atcatcacgc cactcctctg
     4561 cagaatgagt cgtagatgga gagtggatga tgcagggggg gtttgtcgaa gttcaccaga
     4621 ttttcaccag gatgcacgtc atactctttt ttctccggag gcagtgccag catggactgc
     4681 tgctcttcga gccagcgatc gcagggacgg gcctggattg tttcatgctt tcgttggtta
     4741 gcgacatcgt gcagccagcg cagaccgtgg cggttggctg tttcaacatc gacagtgatc
     4801 cccatcgggc gcaggcgagt cattagtggg atgtaaaaac tgttacgggt gtactgcacc
     4861 atccgttcca ccttaccttt agtctgtgcc ctgaaggggc gacacagtcg gggagagaag
     4921 cccatctcct tgccgaactg ccacagcgaa ggatggaacc ggtgctgacc ggtctgatat
     4981 gcgtcacgtt gcagaaccac agttttcata ttgtcataca acacttcgcg cggcacacca
     5041 ccaaagaagc ggaacgcatt acgatggcag gtttccagcg tgtcataacg catattgtca
     5101 gtgaattcga tgtacaacat tcggctgtat ccgagaacag caacgaacac gtgaagcggt
     5161 gagcggccat tacgcatagt gccccagtca acctgcatct gtcgtccggg ttcagtttcg
     5221 aaccgaacgg caggctcctg ctcctgagga accgagagag aacgaatgaa tcccctgaga
     5281 atggtcattc cgccacgata tccctggtct ctgatctcgc gagcgattac cgttgccggg
     5341 attttgtaag gatgagcatc ggcgatgcgt tgacgaatat aatcccggta ttcatccagg
     5401 agtgaagcaa cagcaggtcg cggcgtatat tttggcggct cagattttgc ctgcaaataa
     5461 cgtttaaccg tattgcggga gatccccagt tctctggcaa tcgcccggct actcattccc
     5521 tgcttgtgca ggattttaat ttccataact gtctcaaaag tgaccataag ctctcctgaa
     5581 tcaggagagc agattacccc ctggatctga tttcaggcgt tgggtgtgga tcactattgc
     5641 accgttcgtg acacaatggg ccatacgggc cgacgttcca tgccgcgtta ctacattcag
     5701 cccggacgta aaatgcgccc gtcaaaaaga acggttcgct tcgaaactca gccaggatac
     5761 cacctctccg ccacacccgg gaagaagttg atatggtggt tgccggacaa cagagctcct
     5821 ggattgactg ctgtatcact caaccacgct gaatatcaaa ggagagcgtt accggttaaa
     5881 agagaaacgt aaagctgtgg tgatgaccca aaacgcaacg cctgtcagtg aggatgaaat
     5941 ggtgaaaagc ggacagcatc agtaacggaa gtatcttagc gggtacgaaa atggcaggat
     6001 cgtgacattg acagaatatc tcctgatcat cctgaatgac tgcagttgct gtggaaaagt
     6061 aagctgtctc cacccacggc acgacttaac aggcattccg taactccccc agacttacta
     6121 tgacgcatta tcggtgcact taaaaccgca gaccgcattg cgggtgttac tgcatgacag
     6181 tctgaccgta ttgtgagatg tcggcaggaa ccgatgtccg cgcagggggg aacttcctgc
     6241 agtacctgac gcttcaaata gtattaaccg tagggcgttg ttggttccgg atcgcggaaa
     6301 tgggagcaca ggtctgctac ggggtgtacc tctgacagta aatccggctc gctcatctga
     6361 cagcctctct cccggaaaag accaaaaatg agatcgtctc ctcaacagca gagaagaggt
     6421 cttgacagac aggctacatt gcatacgatt tatgaagaaa aagacagcaa atatggaggt
     6481 tatagcggag aacaccctac gaaattccta tgaaaaacga ttgaaaaaaa tatcaaattc
     6541 gattcgtttt tatatgcttt ttgggaacgt ggcgatgacg aactgttgca tgtgcagagg
     6601 agaatgcacc cggaagcacc agaatcatcc agaaaggcag aacgattacg catcaaacca
     6661 ggagacagca atatcaaggt aatacactga tttaaaatga atttacagca atacgtgaaa
     6721 acatttttta cttaaaggag acagatgcgc tgaagagagt gcaggtattt ttatataaaa
     6781 atgagaagat tacgggatat atatccggca cactatgacc ggagaatacc agtcaggctt
     6841 aaaaagacaa taaatacagt gagttaaata cacagagaaa acgaaaacgt agtatcagag
     6901 ggtacatctt acaacagaga ctgaatttcc ggtttttcat aaccggcgca ttccggaaag
     6961 tgcaggcggc ccggagctct tctgacgcca gtacctgcca gattgttctg tacattctgt
     7021 acatcatgta cggaggtttt tgtttgcttt cttaaaatct gcaaatgtgg gaagagcagg
     7081 tgaggtgtat tgtgggaaag gaactggatg atatctgcac cagctgtccg tatatcgatg
     7141 ctataaaacg gcacaaacaa cagctcggtg ccatagagga atatacacag tggttaaaaa
     7201 aagaaccacg ggcatcttat ttttttctgt tccggctgta tacccgcatt cacaatacat
     7261 ttttccccaa aaagcaacaa ctcccgttca ctcccggtgg cactcactgc ccggaacctg
     7321 atgtcaccct tcgggactta acgctttccc cgggctacca cagtgattat gcccctcaac
     7381 ccattccgga gatggacagt tctgctgttg ttcccccaac taatgaaaac acctcgcctc
     7441 cggaagatac tcccgacaac actccggccg gaggcaatac cggacaagcg gaaaagacca
     7501 gaaactcagg actcacaccc attcctgaaa aacgttcagg aatgcccccg gaacatctgc
     7561 ggtttgccac agggtttcca ccgcaaccga agattgccgg tcccaaagga aagccgatgc
     7621 gcaccgtgca tccggataaa atctaccgtg aaattatatg gttctgctcc ggttacctgc
     7681 ggaaatccgg accagaggct acgaggacga tcatcaactc aatattctgt gaatgggcca
     7741 gcatcttcaa tgattactcc tccccctttt cgtgggtgga cagcagggac agtgaacagt
     7801 gtgactggtt atggaacgcc atgcaggtcc ggtgtgtggg aaccccgctg aatcccctta
     7861 ccccggagca gaaatactgg ttcgcctgcg ccacgtttga taactgggaa ggctggaatg
     7921 agcaacaggt acagttttta ctggaaagta atcccagacg gaacagagcg aagtttacgc
     7981 aggcctcctt ccaggccccc aggattcagc ataaagcgat tcttcttgat gagctgaaaa
     8041 gtgcccggga gcaacaaaaa aggcgtgatg aacgtgctga tggttccgtc ccgctgaaac
     8101 tgtccggaaa aatccacaaa cagcttgaaa gtattgcccg aagtcgtggt gtcctcccaa
     8161 aaaaactgct gaatgaaatg attgagcagg cgtaccagga ctttgtggca aatgagcaac
     8221 acaagacact gagttaagga ccgcagggcc tccggttacc gattctgcag catacacaac
     8281 gaatgtgtcc ggctcttacc tgtgggggcc gcactaaagt tacgcattga gtatgagtgg
     8341 gtaatcccgg atgagaatat cccggttttt acactattca ctgaaggtgc gacatatcat
     8401 tcagtctctc tgaattcttc atttcagtgg ccccggctac agtcgtttaa tctcctccac
     8461 gaaccggggc cgcaacttgt tgtgtcaata ccaaattgtt ggtcattact gtcttgtgtc
     8521 actggcgctg acaagtgaac tgaaatccgc accttaatat tctgactggc tgcgtacatt
     8581 catggcatga agcggccgga gcggctgaac tgaaggtccg ttgtgagcga acagcggaca
     8641 ttcactacac atggttcaaa catgaaccgg agctttattg tctagctgtc agtgtactgc
     8701 gatttacccg ctcggctgtc cagaaaatcg gtctgaaatc cagtcattaa agggccttag
     8761 cctgacatta agcaacttgg taggcggata gataatagaa ataggaatgc gcaggtctgt
     8821 catgttgcct agaacggtga caagctgtcc ggtgcgaaat cattccggca gcgacaggct
     8881 catgtgtcag cccgacgccc agaccgtcaa cgagcatgtg gatctgcgag gcgttatcgt
     8941 tcgcagcgat gttaaaacga acaatctgct catcttctag aagcacaagt gctttaatga
     9001 cattaaggcg gtctatatgt tgcattacag acttcctatt gatctttacc tgaggctgaa
     9061 gcaaccacgt taatccgact atttttcgat tgcaccatcc actatcacca tcgatcttcc
     9121 ctgagcacaa cgttcaactc tcccgcgtac gccgtatacc tgcgcaaggc tctctgcatg
     9181 gatcaccgtt tgcgggtcgc cgctgtcgat aagtttcccc tctttcagca tgatgacctg
     9241 ggcggcatgc cgcagtgcga tattgatatc gtgtaagacg accagcgtaa ccatattgcg
     9301 tcttcgcgtt tcgcgcgata caacatccat cacatggaac tgataattga gatccagcgc
     9361 gctcagtggt tcatccagca ataataatgc ggggcggcga ataagcgact gtgcaagccc
     9421 caccagctgc ttctgaccgc cggacaggct atcgaggtag ttcattgcca gatgtgcgat
     9481 gcctagctct tcgagcagcg ctatagcctg cgcctgattt tgcccggagc cggaggcgcg
     9541 ctgggcgaca accaccgact ccagcacctg taaatgcacg ccctgcggca gggactgcgg
     9601 caggaacaca actttttcag cccgacgagc aaacggcagc gataagagat tctcttcatt
     9661 cagccaggct tcgccactgg cgcgatttag cccggctagg gcgcgcagta gcgttgattt
     9721 accgcagcca ttaggcccca gcagtacagt gacttcgccg cgcggcagcg tagaaatcga
     9781 cagatgctca ataatccgcc gtttgccgta accggcgcac agcgcattaa tcgtaagccc
     9841 gctcatgaca tactccctcg atgacgcatt acaatgctca gaaagaacgg aacgcctacc
     9901 agcgaggtaa caattcctac cggtaaaatg acaccgggaa tgatgttctt cgacgctacc
     9961 gaggccagcg atagcaccag cccaccgata aggatgcttc cgggcaaata aaagcgatga
    10021 tcttctccca gcaacaggcg ggaaatatgc ggtgcaacca ggccaataaa gccaattggc
    10081 cccacaaacg caaccgaaag ggccgccagc aggctgatgc gcagcaggga acccaggcgc
    10141 aaacggcgaa catcaatgcc aaaactcatc gcccgctctt cgccgaggcg tagcgcggta
    10201 agctgccagg cacgacgcag cgaccagggt aaaacaatcg ccatcgccgc cgccagcacc
    10261 gccagtttct cccatgatgc gcgagccagg ctgcccatcg tccagaacac caggccctgc
    10321 aaagtatctt catcggcgac aaactgcata atggacacca gcgcattgaa ggtaaacacc
    10381 agcgcaatcc caaacagcac aacgcccgac gtcggcacgc gcgtccagcg ggtcagacta
    10441 tcgagcagca gggcggacag gagcgcaaaa agaaaagcgt ttgccggtat aaacccgctt
    10501 tcgggaacgc cgggaatacc gatgcccaga acaatcgcca gcgctgcgcc aaaggccgcc
    10561 gccgacgaaa cgccaagcgt aaacgggctg gcgagggagt tattcagaat agtttgcatc
    10621 tctgccccgg ccaaacccag cgccattcct accaggagcg ccatcagcgc atagggcaaa
    10681 cggatgtccc aaacgataac ccgcatgccg ttagtcgccc cggacggatg catcaaagtc
    10741 tttatcagcg aatgtaaagg gagccctgag gggccgaggg tgaaatcgag cagcagcgaa
    10801 gcaatgatag cgataaaaat aaccgccagt attaccagac gctggcgaaa aagctggcga
    10861 tagcggttct cgaccgacag cgtactttcc aatacagtca tggtttttca cagcctccgt
    10921 tagcgcggcg gattgagcac atcgctcatc tgcgcatcgc ttaacgtcag gccaaagaac
    10981 tgccgataaa actcacgcgt ctctttgcgt atatccacat cagcaaatcg cgcaggataa
    11041 agcgttttcg ccagccacaa aaattgcagc gcctcttcgc tggtttcacg gcaccaccag
    11101 aacattcctt tcggattaac gtaaacgcga tgatgaatga ccgcgtccac gcttgcccac
    11161 tgcggagaac tcagaatggc atccgcgtcg cgcttgttca tcgcgatgat aaccgccgga
    11221 ttggcagtga caattttctc cagcgcgact tcacccgaac ggttttgttg atgctcgcca
    11281 aaccaatttt cagcgatatt tttgccgccg gccaggtcaa tccagtcctg gtttagcgag
    11341 ggtctaccgg tggtggtcag cggattgccc atactgtgat agagcgacac gcgctgtgac
    11401 gcaggaagat ctttcaggcg cccggtgacc agggccacgt tgcgatcgaa gtagcgttga
    11461 taggcgagcg ctttttgctg cgcatcgggc cccagcaccg ctgccgtttg ctgtacgcgc
    11521 tgcgttaacg cccgcagtga attcgcctca aacgccagca cctgcacccc agcctgtttc
    11581 agcaacgcct gttgaggcac taccatattc tgcggtacaa aaagcatatc gacccccagc
    11641 gcgataatct gttcgggatt gatttcatgc ccgctgttta cgctaactgc tgcgactttc
    11701 tctatgcgcg gcaggctctg gcgaaacagg gggatccttt ttgcaatcat cgaggttccg
    11761 acaatgctat cgccgtaccc cagcatggca ataatggtat tttgcgccgg ccacggtgac
    11821 gcgatacgca cctccttgtg cgtattttct gcgtgcaggc tggccgccag cagcagcgcc
    11881 agcagcggaa ataaacggga gagtttcatc atttatccat cagaaatcga aggtgacgga
    11941 tgcacgcact tcgcggccgc cgccgataaa cgcacttggg gaaccgttat cgccatcggt
    12001 acccgatggg aagatagaag cccagtaatg tttatcaaac acgttgttta ccaccacgcg
    12061 gaaagtggtt gggacgttgc tcactttcgt ggtgtaacgc gttcccaaat cccatgtggt
    12121 atagctgctg gcgtaagagg tgttggtatc gttcgccgcg cgtttgcccg tataatggac
    12181 gttagcgctg taaacccatt ccggtatgga cggcaaactg tattccgcca acagattggc
    12241 ctgcactttc ggcacgccga caacctgttt attgctggtt gaggcattcg ccgtatcttt
    12301 cagtttcggg tcgaggaagg tcacgccgct gtaaatattc agcccctgcc acacattccc
    12361 ggcagcggtt aactcaaggc cgttgttaac ctggttaccc tgctctttat acacgttatc
    12421 cgtatcaaga taggcaaacg gacgttccag tcggaacagc gcggcgccta gattcatctc
    12481 gccgatgtcc gattttagcc ccacttcata ctgcttgctg cgatacgggt ttagcgtttg
    12541 accggcattt tttacgctct catccgtcgg tgcggtaccg ccctgctcca gcgaatcggc
    12601 atagctaacg taggccattg tgttaggggt aattttatac atcagcgcgg cgttcggact
    12661 taaaccattt tcatcaacct ggttcgtttg attaccgtgc ttatcgtagt ttttagtctg
    12721 cagccagctc tggctgagat agaacattgc cgaccactgc ggcgtaaatg tcaccgtatc
    12781 gccgagcgta acgctctgct gagtatttac actggagcgg tatcgcggcc cgccagtgcg
    12841 gattttgcca tcgccctgct catctatcgc atccgggtga tacatatttg ttgtacccca
    12901 gctatagctg gaacctgttc ctttggcact ataaagcgac cagacatatc ccgtggtaga
    12961 aagtgacaga tcgtggccga tagagccggt atcaatatga ccattcagcc cggcggtgtt
    13021 gctcaggacg cgaaaacgtc cggcagcggt ggaatccttc atcgaacgag agatatcgcc
    13081 ctgattgttg agtattttac tggaaacact acgcatcgcg cggtcagcct gctgccagcc
    13141 cacgccagcg ttcatggacc agtcgtcgtt aaagtaatgg atcaggcgag tgctgatggt
    13201 atcggtagtg aggtcgttgc ccgcagtgct gagcgccaga tttttgtcct tcggattcgg
    13261 cgcagacggc agtttgacgt tcggcccata gttaaagcta ccgacatagc ctttctggat
    13321 aaattcgtaa tggctggcgt cgagctgtag ctgagtgccc ggctgaatat tccagtcgag
    13381 cgcaacggaa acgagtttgc gacgcagcgt gctgtcatcc acattgcctt ccccttcctg
    13441 atcaagcagg ttcacgcgat agccaaaccg tttgttttca tcaaaatggc cacccagatc
    13501 ggcatggccg gtaaacgcac tgcggctttg atagcccagc gtcactttac gcagcgtctc
    13561 ttcggttggg cgcttcgcca cgaaattaaa ctgccctgct gggctcgccg ggccgtacag
    13621 cgcgccggtc aaactgttaa gcacatccat gcgctcaagc atttccaccg gaaacgcggt
    13681 tgttgaaacg atattcagcc cgtccagacg gctgttggcc accacgctac cctgcatccc
    13741 ccgactttgc ggacgcccga catccattcc accgcgcgcc tgcatttgcg tactgggaga
    13801 gtatttcagc atctcgctga cgctttgcgc ctgctgattt tcaatcatct ctttagtaac
    13861 agtggtgctg gaatatggtg tttcgatcca ggatttcgtt cccagcgggc cgacatctat
    13921 atcgttcgtt ttgaaaccgg caccggcggc agaatcggtg gtgtcggcgg cggtgttgcc
    13981 cgtgacaatc atttcatctt cagaagtaga cgaggcatga gcatgaagcg gtatgaaaga
    14041 cgctaatgct ggcaaaagcg cagcgaaagt tatttgagtt ttcatgaatc actgatcgtt
    14101 atgtaaatat ttatattacg atcggtgcat aggcaaaatt atgatccaga tcacacgtga
    14161 atgtaagtta tttacaaatt acgctaacct tctgtctccg ctctttgcag cataaaagta
    14221 tggcggcgcg ttgttgaaag caggagcggc cgggttgtca gaaagctggt ttggacgcaa
    14281 gtaatatata acaactctga ttaccgcata ctgtcaggac agtatctact tgagcgtccg
    14341 cctttggcac aaagcggact gtcagattag attcagacct gtactcatta gctgatacaa
    14401 atcagccaca tatacctgca ttcaaaataa tttcagaaag atattatctc cgtgaatccc
    14461 ccgccacctt tccggtgcac ggggttttgt cttttttcac tgggaataca tgtatgaatc
    14521 cgtctgatgc cattgaggca attgaaaaac cgctctcctc cctgccttac ccgatttccc
    14581 gtcacatcct ggaacatctg cgcaaactca cccgtcacga acccgtgcct ggcattatgg
    14641 gtaaaagcgg ggccggtaaa tcctcactct gtaatgcact gtttcagggg gaggtcaccc
    14701 cggtcagtgc actcatgaca gtgcttcccg gacatgccgc catccatctg atgacacgcc
    14761 tgcaggacga actgcgcacg gagtctgtcc gcactcagac ccgtgaacag tttaccggtg
    14821 cggtggaccg gatatttgac acggcggaga gtgtctgcat tgcctctgtt gcacgcacgg
    14881 ttctgcgcgc cgtccgtgac tcggtggtct ctgttgcccg cgcggtatgg aactggattt
    14941 tcttctgaac ccggtggtga tgtaccacgc tgttgttaca gatttttaac tccatgaaaa
    15001 cactatgcaa acgcagccat acccggggtg tccccttgcg ttgtgtttct caagttaata
    15061 gaatacatcg atcgttacta ttatatcgat cgttataaac gatcgtttgt agtaaatgac
    15121 ctcctccgtg gttgtcatga aaatgcgtct caggaaggtt aaataatgca cgaaaagaac
    15181 atcgccctgc tttgtgatga agccgaccga cttttgcaac tgaacattaa tctgctccgg
    15241 caaatggttg aggagccaga tgtgttatct gacagtaaga acgaaaacag actgcttttt
    15301 gataaacaga aagcactgaa aagaattgag gagctggagg gcgaacaaat caaaaccgcc
    15361 cgcagggaga tggtgctggc tgttgtcggc acgatgaaag caggcaaatc aaccaccatc
    15421 aacgccattg tggggcagga aattctgcct aaccgtaacc gccccatgac ctctgtaccg
    15481 acgctcatcc gccacgttcc cggaaaaact gagccggttc tccatctgga acatattcag
    15541 cctgtccgca atttattaat cacactgcag gaaaaactcg ccaccccggc aggacagcag
    15601 gtcgcacaga ccctgcagca aaccggggat acccgcgaac tgctggatat tctgacggat
    15661 gatggctggc tcaaaaatga ataccacggg gaggaggaaa tctttaccgg actggcatcg
    15721 ttaaacgatc tggttcgtct tgctgcggca atggggactg aatttccttt tgatgaatac
    15781 gcagaagtgc agaaactgcc ggtgatcgac gtggaattca gccatctggt ggggatggat
    15841 gcatgccagg gaacactcac actgctggat acccccggcc ctaatgaggc cggacaaccg
    15901 cagatggaag tgatgatgcg ggatcaactg cagaaagcct ctgcggttct ggctgtgatg
    15961 gattacaccc agatgaactc aaaagcggat gaagacgtcc gtaaagagct taatgccatt
    16021 gctgacgtat cagccggccg cctgtttgta ctggtcaata aatttgatga gaaagaccgc
    16081 aatggcgatg gggcagatgc cgtacgccag aaagttccgg caatgctgaa cagcgatgtg
    16141 ctgcccgcct cccgcgttta tcccggatcc tcacgccagg catacctggc taaccgtgcg
    16201 cttcatgagt tacggaaaaa cggaaccctt cctgttgatg aagcctgggt cgatgatttt
    16261 gtcagggaag ccttcggtcg catgaaaaaa gattacgtct gtaaagacag cgaactggca
    16321 actgaagggg cgacagacct gtgggaatgc tcacttatcg atcaactgat aacggaagtc
    16381 atactgagtt cacactccag agctgcggca ctggcggttg attctgccgc cgcaaaactg
    16441 atgcagaatg cagaaaatat cagtgaatac ctgtcgttac gccatcaggg gctgatgcaa
    16501 agtattcagt cactgcaggc gcatatcacc agcctgcttg aggatatccg ggaaatagca
    16561 ggctgtcagg agcaggtgac cgctgatgtc agaatggcca tggaggagat cgatgccaga
    16621 acccgggaat tactgacggg ggtctgcacc tcactggaag aggagctgaa tgactatttc
    16681 agaagcggta aacgcaaaga acagcaaatg ctggaggaag aaaattcagc gcagccccgg
    16741 gagagaaacg cattcgcttt ctttcacgat attttcggaa ccggaaatca gcatgacagg
    16801 atgcgggact ttgatcccga cagccccgaa ataaaattca gtgatcgcag ggaagccctt
    16861 gaactgatga cgcaaatcga atcgaccgtg accagcctgc accgtgaggc tgaagcacag
    16921 ttccggcctg agctggagaa aatcgtcagc gggattgaaa caggttttcg tggcacggcc
    16981 ctgtacgcca cagaaaacat tgccggtcgc atcaataccc gcctggagga tgagggcttt
    17041 accgtaaaaa tcagttttcc ggcagtcagt cagttacaaa cccggctcgc ggtaaaaaca
    17101 aatctgagtg cgcttatgga ggaaagaacg gagaccgtca cccgtcgccg tcggcagagt
    17161 ggtttatggg gaaagatttg tggagcgttt ggcaccagtg actggggctg ggaaacctat
    17221 aaagaggatg tgagccgcag tgttatcaat atcaacaccg tcaggaagga agttatgtca
    17281 ctgacccggg catatttcgg ggagctgcag gcatccattg agcaggatat taaccagccc
    17341 gtccgccagg aaatcgatgc ctttttctgc gcattcaggg agaaagttga acaactgcgt
    17401 aacacgctga ttcagagcgc tgaagatcat aaacgcgatc agcaggcaca ggaacggctt
    17461 accaggcgac ttcaggcatt aaatgaaagg gttcctgagc tcattactga cagtaaggcg
    17521 ctgagggaag agctggagac aatgctgtga cttcaccatt tattcaacaa attgctgata
    17581 accgggtatg tcaggtactc acctgtcttc ctgaaaaatt tgtggttgat ttcgcaaacg
    17641 gaatcgatgt tgcacaggag catatccgca cggccggaga gcgcacgttc ttccggcgtt
    17701 taaaagaggg actgaccggc gaaggcgctg cacggcagaa tgccatcaat gcttcggttg
    17761 cacagggcct tgaggcgtcc ctgcgctggc tgacggagct gacaacgtca cttgccacca
    17821 caaactacgc gattacccgg gtaaacgaca gggtcagttc actggtcagt gatactgcca
    17881 ggctggcgca ttattccgca gatacacggg agcagttact catcctggcc gatcaggttc
    17941 accataaact gaatcatctt gaagaaaaac tccaccgtgt tgaccaggtt cagcgggcgc
    18001 agttacatct tgagcagata ttctcatggt ggagcgccgg gcgatatgcg tcattttccc
    18061 ctgccggacg ttgttatgtg gcgcttgaag agcttcgctg gggcgcgttt ggtgatgtga
    18121 tacgtcaggg cgaaacaggc caggttaacc agctactgga tattctcaga cataaagcat
    18181 taacgcagat ggcacaggag agtggcggta gtgcaaccgt gcgtctgaac actctggact
    18241 ggcttggtgg tcagggccgg gagcaggcag ataacgagtg gcatgacgcg attaactggc
    18301 tgggagactg gtgcagtgaa gagcagcatc cggtgatctg gtcaaccacg caggctgcag
    18361 aacacttacc ggttcgcatg cctcgcctct gctctgcaga acgcctctct gaaagtatgg
    18421 tcgatgaaat atttcagaag ggggctgcat gagtacagaa atgaaaacgg ggctggtgct
    18481 gtccggtggc ggggcggtgg gcgcttatca ggcgggagtg gttaaggcac tggcagagtg
    18541 tggtacacag atcagcatgg tttcaggggc cagcattggc gcattcaatg gtgccattat
    18601 cgcggcctct cccgatctgt cagaagctgc cgtacgcctg gaggcgctct gggatcatct
    18661 ggggaataat caggtgctgt cggtaaaccg attggtttac ttttcattgc tgaaaaaatt
    18721 gttccaggca atgaacctct gccagatccc cggacgtgca ggagcactgc ttacgacgct
    18781 tcttcgccat atatcgatac tcaacgggtt tgacaatctg atggctcagc cgttgttgtc
    18841 agatgagccc ctgacagcgc tgttggatca ttatcttgat actgatgctc tggcagacgg
    18901 gctaccgctg tatgtgtcgc tgtaccccac agaagggggc atgcaggata ttattgactg
    18961 cattcgtgct gaactgggtg ccggaaccac gaaaaacgcc gtttttcagc atatccagag
    19021 cctgccccgc ggacagcaga aagaggctct gcttgcgtca gccgcgctgc ccctgctgtt
    19081 ccgtccccgt gaggttcagg ggacaatgtt cggtgatggt ggtatgggag gatggcgaaa
    19141 tatgcaggga aatacccctg tgacgcctct ggtcgatgcc ggatgcaata tggtgattgt
    19201 gacgcatctg agtgacggtt ctttatggga tcgccaggct tttccggaca ccacaatcct
    19261 tgagatccgt ccccggaaaa ggctgaaata tgcaggtgat ggtgacaaca gcggcggtct
    19321 gctcagtttc acgttggcac ataccgacgc ctggcgtcag cagggctatg aagacacgat
    19381 gctgacgatg gagcatatcc ggaaaccgct ggcagcacgt caggtactgt cccggtcaga
    19441 aacggtgttg cagaaaagtc tggagataac ggaagaggca gatttggcac tgagaaacgc
    19501 gatggcccgg attaaataaa gacgccccaa agaaacgcca ccgcctgtgg tggtgttttt
    19561 cctggctggt gttattgaaa atattttcag atatatatta cctgagagaa agttttaccc
    19621 ctcacctgtc aattcactca ctggaaattt aattatgcaa ttacctgtaa agttattaat
    19681 gagccttata tctctggtca gcgttattgc acgtgccggg aaatataaaa attacatccg
    19741 ggatgaaata aaatactggc gatatacatc atacaagggg ggggaatttc cggaaggttt
    19801 cactgatgag aaattttcca gcgccattta caacggaaga atatttacaa tgaaacgttt
    19861 acataccctg atgttatttc tggcggttct gtttactggc tttaacgtgg aagcagcgag
    19921 cgtgaaacaa gcgctcagct gcgacccaaa cgcccgggct gaacaacctg gagcgtgtcc
    19981 aacaacgtac gagttgtacg aaggtgacgc tgcctacaaa gctgcgcttg acaaagcatt
    20041 aaaaccggtc ggactgagcg gcatgttcgg taaaggcggg tatatggatg gccctggcgg
    20101 aaacgtaacg ccagtaacca ttaacggtac agtctggctc cagggcgacg gttgcaaagc
    20161 caatacctgc ggctgggact ttatcgtaac actctataac ccaaaaaccc atgaagtcgt
    20221 tggctaccgc tactttggtt tagatgaccc ggcctacctg gtttggttcg gcgaaattgg
    20281 cgtgcatgaa ttcgcgtatc tggtgaaaaa ctacgtagct gcggttaact aatttaagga
    20341 gtctgtcatg aaaactttta tcaaaacttt actcgttgct gtaactattc tgttctctgt
    20401 cttcgctacg gcgaaacaag taaaactgcc aaacaacatc aaatacgtta atactacaga
    20461 ggcgttttcc tgtactgaga ttgacggtat gaattgccag acgaagaatc cgttcaacta
    20521 taaagataac agctatgttt tcgtgcttga acgtggtggt gcctggtgtt acgactacac
    20581 tgtctcggta cttaacctga aaaccgggaa agcacagatg ctcgaataca aagacaacca
    20641 gctgtgctca ggtagcaaca aaccgttctt cgaaatcaaa aatggcgtac cgacggtagg
    20701 agtcatcgac acatccggaa aacctgtcgt tgtggctctg gacaaactta aaacctgagg
    20761 agtaaaccat gcgtttctct gcacattatt aatatcaaag gtaaatacag ccccagccat
    20821 tgttgccgga aagccgctta atgcgtggct cgccagtgaa aggcttctca gacaataaac
    20881 ctcattcatt tcacccgtca ggccgcgtct tctccgggag acgtggcttt tttcatttat
    20941 tacaccagta aatcttaacc accgataagg agcaaagtat gcgattagca agtcgttttg
    21001 gttatactgc aaaccagata cgtcgtgacc gtccactgac acatgaagag ctgatgcacc
    21061 atgtgccggg gattttcggg gaagagaaac acacgtcccg aagccagaac tatacgtaca
    21121 tccccaccat caccgtactg gaaagcctgc agcgggaagg ctttcagcca ttcttcgcct
    21181 gccagacccg tgtgcgcgac ccgggccgcc ggggatacac caaacacatg ctgcgtctgc
    21241 ggcgggccgg agagataaac ggagagcatg tccctgaaat tattctgctc aactctcatg
    21301 acggtacctc cagctaccag atgctgccgg gttacttcag gttcgtctgc cagaacggct
    21361 gcgtctgtgg tcagtctctg ggggaagtgc gtgttccaca ccggggagac gtagtggaga
    21421 aagttattga aggggcttac gaggtggtgg gcgtgtttga ccggatagag gagaagcgtg
    21481 atgccatgca gtcgctggtc ctgccgccac cggcacgcca ggcgctggca caggcggcac
    21541 tgacttaccg ttatggtgac gaacatcagc ccgtcaccac cgccgacatt ctgacgccac
    21601 gacgccggga ggattacggt aaggacctgt ggagtgctta tcagaccatc caggagaata
    21661 tgctgaaagg cgggatttcc ggtcgcagtg caaaaggaaa acgtatccat acccgggcca
    21721 ttcacagcat tgataccgac attaagctca accgtgcatt gtgggtgatg gcagaaacga
    21781 tgctggagag cctgcgctga tgcaatctgt ccctgaaggc gtgattcagg caccagaatc
    21841 acccgacgcc ccggagagct cagtctgtgc agactgagta caggcaatgg catctggcca
    21901 tccgtctgtg accggaacgg ttacgcgtac ggctgtccgg aatttcagcc gaatatcatt
    21961 catccctgac attaccgggt ctcacggtca gtgatgaacg tgatgttgtc agcccccggc
    22021 ctttactccc tctcatttat tcacattaca aggatttcat acatgaaaac attgtctcag
    22081 aacaccacct cttcggcctg tgcaccggag actggcctgc aacaactggt tgccaccata
    22141 gtccctgatg aacagcgcat cagcttctgg ccgcagcatt ttggcctcat tccacagtgg
    22201 gtgaccctgg agccccgggt cttcggctgg atggaccgtc tgtgcgaaga ctactgcggt
    22261 ggtatctgga atctgtacac cctgaacaac ggcggggcat ttatggcccc cgaaccggat
    22321 gacgatgatg acgaaacatg ggtactgttc aatgccatga acggtaaccg cgctgaaatg
    22381 agcccggaag ccgccggcat tgccgcctgt ctgatggcgt acagccatca cgcctgtcgt
    22441 acagagaatt atgccatgac ggttcattat taccggctgc gggattatgc cctgcagcat
    22501 ccggaatgca gcgccattat gcgcatcatc gactgaatgg aggcagggac aatgcaacag
    22561 ctttcctttc tgcccggaga gatgacgccc ggcgagcgca gcctcattca gcgggccctg
    22621 aaaactctgg accgccatct tcatgaaccc ggcgtggcct tcacctccac ccgtgcggca
    22681 cgggaatggc tgattctgaa catggcggga ctggagcgtg aagagttccg ggtgctgtat
    22741 ctgaacaacc agaatcagct gattgccggt gaaaccctct tcaccggcac catcaaccgc
    22801 acggaagtcc atccccggga agtgattaaa cgcgccctgt atcacaatgc cgctgccgtg
    22861 gtgctggcgc acaatcaccc gtccggtgaa gtcacaccca gtaaggcaga ccggcttatc
    22921 accgaacgtc tggtacaggc actgggcctg gtggatatcc gggtgccgga ccatctgata
    22981 gtcggtggca accaggtttt ctcctttgcc gaacatggtc tgctttaacc cgtcacaacc
    23041 acatcacacc tgttttcact tttatcttct gtcttcagag gaatcccatt atgaaaatta
    23101 tcacccgtgg tgaagccatg cgtattcacc aacaacatcc gacatcccgt ctttttccgt
    23161 tctgtaccgg taagtaccgc tggcacggca gtgctgaagc gtataccggt cgtgaagtgc
    23221 aggatattcc cggcgtgctg gctgtgtttg ccgaacgccg taaggacagt tttggcccgt
    23281 atgtccggct gatgagcgtc actctgaact gagtcagtaa gggcattctg atgagcagaa
    23341 ttatcgccac taccggacca ttcttagccg attttctgta aggattttat cgtgtcagac
    23401 acactccccg ggacaacact tcccgacgac aatcacgacc gcccctggtg ggggctgccc
    23461 tgcaccgtga cgccctgttt cggggcacgt ctggtgcagg agggtaaccg gttgcattac
    23521 cttgccgacc gcgccagtat cagaggcctg ttcagcgatg cagatgcgta ccacccggac
    23581 caggcctttc cgctgctgat gaaacaactg gaactcatgc tcaccagcgg tgaactgaat
    23641 ccccgccatc agcataccgt cacgctgtat gcaaaagggc tgacctgcaa agccgatacc
    23701 ctcagcagtt gtggttacgt ttatctggct gtttatccga cgcccgaaat gaaaaattaa
    23761 ctctccagaa tagccttctg ctacggcctg gtgttttcac cacgccactt ttccattttt
    23821 atatctgcat atcaggaaaa tcttcagtat gaaaacatta cctgtattac ccgggcaggc
    23881 ggccagttct cgcccgtctc ctgttgaaat ctggcagata ctgctgtccc gactactgga
    23941 ccagcactat ggcctcacac tgaatgacac accttttgcc gatgaacgtg tgattgagca
    24001 gcatattgag gcaggcattt cactgtgtga tgcagtgaac tttctcgttg aaaaatacgc
    24061 gctggtgcgt accgaccagc cgggattcag cgcctgtacc cgctctcagt taataaacag
    24121 catcgatatc ctccgggctc gcagggcgac cggcctgatg acccgcgaca attacagaac
    24181 ggtaaataac attaccctgg gtaagtatcc ggaggcgaaa tgatgacact ggaagccgac
    24241 agcgttaacg tacaggcgct ggatatgggg cacattgtcg ttgacattga tggtgttaat
    24301 atcactgaac tgattaataa ggccgctgaa aacggttatt cactccgcgt ggtggatggc
    24361 cgtgactcta ccgaaacacc ggcaacttat gccagccctc accagttgct gtaagacaat
    24421 gcagtgatac agggtatagt gccattaacc acagaaacag cactgttgtc ctgtattaag
    24481 caggcaagag aagatgtcct taccctgcgc catctgcagc ttctgcacca gaaccggtga
    24541 atctcactca acaggcttca tttcccctga cgccagcctg aatacagctg gcgttttcat
    24601 ttataaacag aaaggaaaac cgataataat ggaatggacg ccccgaccat gaagcaacgg
    24661 taggatatta ctgataaacc ttccagtgga ggtgcgttat ggccgtttta acgattagca
    24721 ttgacctgac aaaaaatgaa ttccaaatcc acggtctggg taggaacaga aaaatctaag
    24781 ctcagaaaac ggattaaagt aagcgtcaac ggagca
//
LOCUS       DAHHTG010000067        24700 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.067, whole genome shotgun sequence.
ACCESSION   DAHHTG010000067 DAHHTG010000000
VERSION     DAHHTG010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 24700)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24700)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24700
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.067"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            complement(<1..804)
                     /locus_tag="NBI74_002650"
     CDS             complement(<1..804)
                     /locus_tag="NBI74_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001556376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HCD1932962.1"
                     /translation="MTGKLRFEVNDNQGCFIFPETWFGSLLDEFEELIDAYDADEISE
                     TSYINKLRRLARQENDFIDVHAHLAYVFLEQNAPRKALNAALKGLAIGNRLIPEGFSG
                     RIIWIHPDNRPFLRALYAAILANAHLQRHQDAIMLIEKILDYNPEDNHGARWLLGPEL
                     LRTGAHEQARHILQEHADEFSPYWYELGLLHFLNGELVKAATAFRRGFAANTYIAEIL
                     CGNLHPFPLAVWHNFSGGPDTAEDYYATYHPLWGQYPEALLFVNWLYNHS"
     gene            complement(1060..2325)
                     /locus_tag="NBI74_002651"
     CDS             complement(1060..2325)
                     /locus_tag="NBI74_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
                     /protein_id="HCD1932963.1"
                     /translation="MALTDAKIRAAKPTDKAYKLTDGAGMFLLVHPNGSRYWRLRYRI
                     LGKEKTLALGVYPEVSLSEARTKRDEARKLISEGVDPCEQKRAKKVVPDLQLSFEHIA
                     RRWHASNKQWAQSHSDKVLKSLETHVFPFIGNRDITTLSTPDLLIPVRAAEVKQIYEI
                     ASRLQQRISAVMRYAVQSGIIRYNPALDMAGALTTVKRQHRPALDLSRLPELLSRIDG
                     YKGQPVTRLAVMLNLLVFIRSSELRYARWSEIDIDNAMWTIPAEREPLPGVKFSYRGS
                     KMRTPHLVPLSQQAVAILAELQTWAGENGLIFTGAHDPRKPISENTVNKALRVMGYDT
                     TKEVCGHGFRAMACSALIESGLWSRDAVERQMSHQERNGVRAAYIHKAEHLEERRLML
                     QWWADFLDANREECISPFEYAKVNNPLKR"
     gene            complement(2523..2598)
                     /locus_tag="NBI74_002652"
     tRNA            complement(2523..2598)
                     /locus_tag="NBI74_002652"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2563..2565),aa:Phe,seq:gaa)
     gene            complement(2704..3411)
                     /locus_tag="NBI74_002653"
     CDS             complement(2704..3411)
                     /locus_tag="NBI74_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HCD1932964.1"
                     /translation="MVIGPFINASAVLLGGVLGALLSQRLPERIRVSMTSIFGLASLG
                     IGILLVVKCANLPAMVLATLLGALIGEICLLEKGVNTAVTKAQNLFRHSRKKPAHESF
                     IQNYVAIIVLFCASGTGIFGAMNEGMTGDPSILIAKSFLDFFTAMIFACSLGIAVSVI
                     SIPLLIIQLTLAWAAALILPLTTPSMMADFSAVGGLLLLATGLRICGIKMFPVVNMLP
                     ALLLAMPLSAAWTAWFA"
     gene            3804..5939
                     /gene="speC"
                     /locus_tag="NBI74_002654"
     CDS             3804..5939
                     /gene="speC"
                     /locus_tag="NBI74_002654"
                     /EC_number="4.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase"
                     /protein_id="HCD1932965.1"
                     /translation="MKSMNIAASSELVSRLSTHRRVVALADTDFTDVAAVVITAADSR
                     SGILALLKRTGFHLPVFLYSEHAVELPAGVTAVINGNEQQWLELESAACQYEENLLPP
                     FYDTLTQYVEMGNSTFACPGHQHGAFFKKHPAGRHFYDFFGENIFRADMCNADVKLGD
                     LLIHEGSAKDAQKFAAKVFHADKTYFVLNGTSAANKVVTNALLTRGDLVLFDRNNHKS
                     NHHGALIQAGATPVYLEASRNPFGFIGGIDAHCFNEEYLRQQIRDVAPEKADLPRPFR
                     LAIIQLGTYDGTVYNARQVIDTVGHLCDYILFDSAWVGYEQFIPMMADSSPLLLELNE
                     NDPGIFVTQSVHKQQAGFSQTSQIHKKDNHIRGQARFCPHKRLNNAFMLHASTSPFYP
                     LFAALDVNAKIHEGESGRRLWAECVELGIESRKAILARCKLFRPFIPPVVDGKLWQDY
                     PTSVLASDRRFFSFEPGANWHGFEGYAADQYFVDPCKLLLTTPGIDAETGEYSDFGVP
                     ATILAHYLRENGIVPEKCDLNSILFLLTPAESHEKLAQLVAMLAQFEQHIEDDSPLAE
                     VLPSVYNKYPVRYRDYTLRQLCQEMHDLYVSFDVKDLQKAMFRQQSFPSVVMNPQDAH
                     SAYIRGEVELVRIRDAEGRIAAEGALPYPPGVLCVVPGEVWGGAVQRYFLALEEGVNL
                     LPGFSPELQGVYSETDADGMKRLYGYVLK"
     gene            complement(5988..7244)
                     /gene="nupG"
                     /locus_tag="NBI74_002655"
     CDS             complement(5988..7244)
                     /gene="nupG"
                     /locus_tag="NBI74_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupG"
                     /protein_id="HCD1932966.1"
                     /translation="MNLKLQLKILSFLQFCLWGSWLTTLGSYMFVTLKFDGASIGAVY
                     SSLGIAAVFMPALLGIVADKWLSAKWVYAICHTIGAITLFMAAEVTTPEAMFLVILIN
                     SFAYMPTLGLINTISYYRLQNAGMDIVTDFPPIRIWGTIGFIMAMWVVSLSGFELSHM
                     QLYIGAALSAILVLFTLTLPHIPVAKQQANQSWTTLLGLDAFALFKNKRMAIFFIFSM
                     LLGAELQITNMFGNTFLHSFDKDPMFASSFIVQHASIIMSISQISETLFILTIPFFLS
                     RYGIKNVMMISIVAWILRFALFAYGDPTPFGTVLLVLSMIVYGCAFDFFNISGSVFVE
                     KEVSPAIRASAQGMFLMMTNGFGCILGGIVSGKVVEMYTQNGTTDWQTVWLIFAGYSV
                     VLAFAFMAMFKYKHVRVPTGTQTVSH"
     gene            complement(7446..8525)
                     /gene="mltC"
                     /locus_tag="NBI74_002656"
     CDS             complement(7446..8525)
                     /gene="mltC"
                     /locus_tag="NBI74_002656"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltC"
                     /protein_id="HCD1932967.1"
                     /translation="MKKYLALALIAPLLISCSTTKKGDTYNEAWVKDTNGFDILMGQF
                     AHNIENIWGFKEVVIAGPKDYVKYTDQYQTRSHINFDDGTITIETIAGTEPAAHLRRA
                     IIKTLLMGDDPSSVDLYSDVDDITISKEPFLYGQVVDNTGQPIRWEGRASNFADYLLK
                     NRLKSRSNGLRIIYSVTINMVPNHLDKRAHKYLGMVRQASRKYGVDESLILAIMQTES
                     SFNPYAVSRSDALGLMQVVQHTAGKDVFRSQGKSGTPSRSFLFDPASNIDIGTAYLAM
                     LNNVYLGGIDNPTSRRYAVITAYNGGAGSVLRVFSNDKIQAANIINTMTPGDVYQTLT
                     TRHPSAESRRYLYKVNTAQKSYRRR"
     gene            complement(8589..8864)
                     /locus_tag="NBI74_002657"
     CDS             complement(8589..8864)
                     /locus_tag="NBI74_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HCD1932968.1"
                     /translation="MSRTIFCTFLQREAEGQDFQLYPGELGKRIYNEISKEAWAQWQH
                     KQTMLINEKKLNMMNAEHRKLLEQEMVNFLFEGKEVHIEGYTPEDKK"
     gene            complement(8892..9944)
                     /gene="mutY"
                     /locus_tag="NBI74_002658"
     CDS             complement(8892..9944)
                     /gene="mutY"
                     /locus_tag="NBI74_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HCD1932969.1"
                     /translation="MQASQFSAQVLDWYDKYGRKTLPWQIDKTPYKVWLSEVMLQQTQ
                     VATVIPYFERFMARFPTVTDLANAPLDEVLHLWTGLGYYARARNLHKAAQQVATLHSG
                     KFPETFEEVAALPGVGRSTAGAILSLSLGKHFPILDGNVKRVLARCYAVSGWPGKKEV
                     ENKLWSLSEQVTPAVGVERFNQAMMDLGAMICTRSKPKCSFCPLQNGCIATANNSWSL
                     YPGKKPKQTLPERTGYFLLLQHEDEVLLAQRPPSGLWGGLYCFPQFADEESLRQWLAQ
                     RQIVADNLTQLTAFRHTFSHFHLDIVPMWLPVSSFTGCMDEGNALWYNLAQPPSVGLA
                     APVERLLQQLRTGAPV"
     gene            10105..10824
                     /gene="trmB"
                     /locus_tag="NBI74_002659"
     CDS             10105..10824
                     /gene="trmB"
                     /locus_tag="NBI74_002659"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HCD1932970.1"
                     /translation="MKNDVISPEFDENGRPLRRIRSFVRRQGRLTKGQEHALENYWPV
                     MGVEFSEDMLDFPALFGREAPVTLEIGFGMGASLVAMAKDRPEQDFLGIEVHSPGVGA
                     CLASAHEEGLSNLRVMCHDAVEVLHKMIPDNSLRMVQLFFPDPWHKARHNKRRIVQVP
                     FAELVKSKLQLGGVFHMATDWEPYAEHMLEVMSSIDGYKNLSESNDYVPRPASRPVTK
                     FEQRGHRLGHGVWDLMFERVK"
     gene            10824..11150
                     /locus_tag="NBI74_002660"
     CDS             10824..11150
                     /locus_tag="NBI74_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggL family protein"
                     /protein_id="HCD1932971.1"
                     /translation="MAKNRSRRLRKKMHIDEFQELGFSVAWRFPEGTSEEQIDKTVDD
                     FINEVIEPNKLAFDGSGYLAWEGLICMQEIGKCTEEHQAIVRKWLEERKLDEVRTSEL
                     FDVWWD"
     gene            11334..12053
                     /locus_tag="NBI74_002661"
     CDS             11334..12053
                     /locus_tag="NBI74_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2884 domain-containing protein"
                     /protein_id="HCD1932972.1"
                     /translation="MMRKMLLAAALSVTAMTAHADYQCSVTPRDDVIVSPQTVQVKGE
                     NGNLVITPDGNVMYNGKQYSLNAAQREQAKDYQAELRSTLPWIDEGAKSRVEKARIAL
                     DKIIVQEMGESSKMRSRLTKLDAQLKEQMNRIIETRSDGLTFHYKAIDQVRAEGQQLV
                     NQAMGGILQDSINEMGAKAVLKSGGNPLQNVLGSLGGLQSSIQTEWKKQEKDFQQFGK
                     DVCSRVVTLEDSRKALVGNLK"
     gene            12229..13275
                     /gene="ansB"
                     /locus_tag="NBI74_002662"
     CDS             12229..13275
                     /gene="ansB"
                     /locus_tag="NBI74_002662"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparaginase 2"
                     /protein_id="HCD1932973.1"
                     /translation="MEFFKKTALAALVMGFSGAALALPNITILATGGTIAGGGDSATK
                     SNYTAGKVGVENLVNAVPQLKDIANVKGEQVVNIGSQDMNDNVWLTLAKKINTDCDKT
                     DGFVITHGTDTMEETAYFLDLTVKCDKPVVMVGAMRPSTSMSADGPFNLYNAVVTAAD
                     KASANRGVLVVMNDTVLDGRDVTKTNTTDVATFKSVNYGPLGYIHNGKIDYQRTPARK
                     HTSDTPFDVSKLNELPKVGIVYNYANASDLPAKALVDAGYDGIVSAGVGNGNLYKSVF
                     DTLATAAKNGTAVVRSSRVPTGATTQDAEVDDAKYGFVASGTLNPQKARVLLQLALTQ
                     TKDPQQIQQIFNQY"
     gene            13392..14399
                     /locus_tag="NBI74_002663"
     CDS             13392..14399
                     /locus_tag="NBI74_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1202 family protein"
                     /protein_id="HCD1932974.1"
                     /translation="MKKQWIVGTALLMLMTDNVWADGEPPTENILKDQFKKQYHGILK
                     LDAITLKNLDAKGNQATWSAEGDVSSSDDLYTWVGQLADYELLEQTWTKDKPVKFSAM
                     LTSKGTPASGWSVNFYSFQAAASDRGRVVDDIKTNNKYLIVNSEDFNYRFSQLESALN
                     NQKNSIPALEKEVKALDKQMVAAQKAADAYWGKDANGKQMTREDAFKKIHQQRDEFNK
                     QNDSEAFAVKYDKEVYQPAIAACHKQSEECYEVPIQQKRDFDINEQRRQTFLQSQKLS
                     RKLQDDWITLEKGQYPLTMKVSEINSKKVVILMKIDDINQANERWKKDTEQLRRNGVI
                     K"
     gene            complement(14468..15604)
                     /gene="hemW"
                     /locus_tag="NBI74_002664"
     CDS             complement(14468..15604)
                     /gene="hemW"
                     /locus_tag="NBI74_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HCD1932975.1"
                     /translation="MVKLPPLSLYIHIPWCVQKCPYCDFNSHALKGEVPHDDYVQHLL
                     NDLDNDVAYAQSREVKTIFIGGGTPSLLSGPAMQTLLDGVRARLPLAADAEITMEANP
                     GTVEADRFVDYQRAGVNRISIGVQSFSEEKLKRLGRIHGPQEAKRAAKLASGLGLRSF
                     NLDLMHGLPDQSLEEALGDLRQAIELNPPHLSWYQLTIEPNTLFGSRPPVLPDDDALW
                     DIFEQGHQLLTAAGYQQYETSAYAKPGYQCQHNLNYWRFGDYIGIGCGAHGKVTFLDG
                     RILRTTKTRHPRGFMQGRYLESQRDVDAADKPFEFFMNRFRLLEAAPRVEFSQYTGLS
                     EEVIRPQLEEAIAQGYLTECADYWQITEHGKLFLNSLLELFLAE"
     gene            complement(15597..16190)
                     /locus_tag="NBI74_002665"
     CDS             complement(15597..16190)
                     /locus_tag="NBI74_002665"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XTP/dITP diphosphatase"
                     /protein_id="HCD1932976.1"
                     /translation="MQKVVLATGNAGKVRELASLLSDFGLDIVAQTDLGVDSAEETGL
                     TFIENAILKARHAAKVTGLPAIADDSGLAVDVLGGAPGIYSARYSGEDATDQKNLQKL
                     LETLKDVPDDQRQARFHCVLVYLRHAEDPTPLVCHGSWPGVITREPAGTGGFGYDPIF
                     FVPSEGKTAAELTREEKSAISHRGQALKLLLDALRNG"
     gene            complement(16198..16488)
                     /locus_tag="NBI74_002666"
     CDS             complement(16198..16488)
                     /locus_tag="NBI74_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggU family protein"
                     /protein_id="HCD1932977.1"
                     /translation="MSAVTVNDDGLVLRLYIQPKASRDSIVGLHGDEVKVAITAPPVD
                     GQANSHLVKFLGKQFRVAKSQVVIEKGELGRHKQIKIINPQQIPPEIAALIN"
     gene            complement(16485..17051)
                     /gene="yggT"
                     /locus_tag="NBI74_002667"
     CDS             complement(16485..17051)
                     /gene="yggT"
                     /locus_tag="NBI74_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotic shock tolerance protein YggT"
                     /protein_id="HCD1932978.1"
                     /translation="MNTLTFLLSTVIELYTMVLLLRIWMQWAHCDFYNPFSQFVVKVT
                     QPIIGPLRRVIPAMGPIDSASLLVAYILSFIKAIVLFKVVTFLPIIWIAGLLILLKTI
                     GLLIFWVLLVMAIMSWVSQGRSPIEYVLIQLADPLLRPIRRLLPAMGGIDFSPMILVL
                     LLYVINMGVAEVLQATGNMLLPGLWMAL"
     gene            complement(17069..17773)
                     /gene="yggS"
                     /locus_tag="NBI74_002668"
     CDS             complement(17069..17773)
                     /gene="yggS"
                     /locus_tag="NBI74_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate homeostasis protein"
                     /protein_id="HCD1932979.1"
                     /translation="MNDIAHNLAQVRDKISAAATRCGRSPEEITLLAVSKTKPASAIA
                     EAIDAGQRQFGENYVQEGVDKIRHFQELGVTGLEWHFIGPLQSNKSRLVAEHFDWCHT
                     IDRLRIATRLNDQRPAELPPLNVLIQINISDENSKSGIQLAELDELAAAVAELPRLRL
                     RGLMAIPAPESEYVRQFEVARQMAVAFAGLKTRYPHIDTLSLGMSDDMEAAIAAGSTM
                     VRIGTAIFGARDYSKK"
     gene            17791..18771
                     /locus_tag="NBI74_002669"
     CDS             17791..18771
                     /locus_tag="NBI74_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HCD1932980.1"
                     /translation="MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGRMEAAPFDAPDV
                     EELLREWLDDDQRAILLENGQLDFAVSLAENQRLRGSAFAQRQGISLALRLLPSHCPQ
                     LEQLGAPPVLPELLKSENGLILVTGATGSGKSTTLAAMVGYLNQHADAHILTLEDPVE
                     YLYASQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETG
                     HLVLATLHTRGAAQAVERLVDSFPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVAL
                     FELLVNTPAVGNLIREGKTHQLLHVIQTGQQVGMLTFQQSYQQRVGEGRL"
     gene            complement(18950..19366)
                     /gene="ruvX"
                     /locus_tag="NBI74_002670"
     CDS             complement(18950..19366)
                     /gene="ruvX"
                     /locus_tag="NBI74_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HCD1932981.1"
                     /translation="MSGTLLAFDFGTKSIGVAVGQRITGTARPLPAIKAQDGTPDWNL
                     IERLLKEWQPDEIIVGLPLNMDGTEQPLTARARKFANRIHGRFGVEVKLHDERLSTVE
                     ARSGLFEQGGYRALNKGKVDSASAVIILESYFEQGY"
     gene            complement(19366..19929)
                     /locus_tag="NBI74_002671"
     CDS             complement(19366..19929)
                     /locus_tag="NBI74_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HCD1932982.1"
                     /translation="MNLQHHFLIAMPALQDPIFRRSVVYICEHNTNGAMGIIVNKPLE
                     NLKIEGILEKLKITPEPRDESIRLDKPVMLGGPLAEDRGFILHTPPSNFASSIRISDN
                     TVMTTSRDVLETLGTDKQPSDILVALGYASWEKGQLEQEILDNAWLTAPADLNILFKT
                     PIADRWREAAKLIGVDILTMPGVAGHA"
     gene            complement(20038..20988)
                     /gene="gshB"
                     /locus_tag="NBI74_002672"
     CDS             complement(20038..20988)
                     /gene="gshB"
                     /locus_tag="NBI74_002672"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HCD1932983.1"
                     /translation="MIKLGIVMDPIANINIKKDSSFAMLLEAQRRGYELHYMEMADLY
                     LINGEARARTRTLSVEQNYDKWYEFTGEQDLPLADLDVILMRKDPPFDTEFIYATYIL
                     ERAEEKGTLIVNKPQSLRDCNEKLFTAWFSDLTPETLVTRNKAQLKAFWEKHSDIILK
                     PLDGMGGASIFRVKEGDPNLGVIAETLTEHGTRYCMAQNYLPAIKDGDKRVLVVDGEP
                     VPYCLARIPQGGETRGNLAAGGRGEPRPLTESDWKIARQIGPTLKEKGLIFVGLDIIG
                     DRLTEINVTSPTCIREIEAEFPVSITGMLMDAIEARLQQQ"
     gene            complement(21001..21732)
                     /gene="rsmE"
                     /locus_tag="NBI74_002673"
     CDS             complement(21001..21732)
                     /gene="rsmE"
                     /locus_tag="NBI74_002673"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014226833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HCD1932984.1"
                     /translation="MRIPRIYHPEPLTSHSHIALCEDAANHIGRVLRMGPGQVLQLFD
                     GSNQVFDAEITSASKKSVEVKVLEGQIDDRESPLHIHLGQVMSRGEKMEFTIQKSIEL
                     GVSLITPLFSERCGVKLDSERLNKKLQQWQKIAIAACEQCGRNRVPEIRPVMDLEAWC
                     AEQDEGLKLNLHPRASNSINTLPLPVERVRLLIGPEGGLSADEIAMTARYQFTDILLG
                     PRVLRTETTALTAITALQVRFGDLG"
     gene            complement(21812..22519)
                     /gene="endA"
                     /locus_tag="NBI74_002674"
     CDS             complement(21812..22519)
                     /gene="endA"
                     /locus_tag="NBI74_002674"
                     /EC_number="3.1.21.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease I"
                     /protein_id="HCD1932985.1"
                     /translation="MYRYLSIAAVVLSAAFSGPALAEGINSFSQAKAAAVKVHADAPG
                     TFYCGCKINWQGKKGVVDLQSCGYQVRKNENRASRVEWEHVVPAWQFGHQRQCWQDGG
                     RKNCAKDPVYRKMESDMHNLQPSVGEVNGDRGNFMYSQWNGGEGQYGQCAMKVDFKEK
                     VAEPPARARGAIARTYFYMRDQYNLTLSRQQTQLFNAWDKMYPVTDWECERDERIAKV
                     QGNHNPYVQRACQARKS"
     gene            complement(22614..23111)
                     /locus_tag="NBI74_002675"
     CDS             complement(22614..23111)
                     /locus_tag="NBI74_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HCD1932986.1"
                     /translation="MKTSRLPIAIQQAVMRRLREKLAQANLKLGRNYPEPKLSYTQRG
                     TSAGTAWLESYEIRLNPVLLLENSEAFIEEVVPHELAHLLVWKHFGRVAPHGKEWKWM
                     MESVLGVPARRTHQFELQSVRRNTFPYRCKCQEHQLTVRRHNRVVRGEAVYRCVHCGE
                     QLVAK"
     gene            complement(23188..24582)
                     /gene="galP"
                     /locus_tag="NBI74_002676"
     CDS             complement(23188..24582)
                     /gene="galP"
                     /locus_tag="NBI74_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/proton symporter"
                     /protein_id="HCD1932987.1"
                     /translation="MPDAKKQGRSNKAMTFFVCFLAALAGLLFGLDIGVIAGALPFIA
                     DEFQITSHTQEWVVSSMMFGAAVGAVGSGWLSFKLGRKKSLMIGAILFVAGSLFSAAA
                     PNVEVLILSRVLLGLAVGVASYTAPLYLSEIAPEKIRGSMISMYQLMITIGILGAYLS
                     DTAFSYTGAWRWMLGVIIIPAILLLIGVFFLPDSPRWFAAKRRFVDAERVLLRLRDTS
                     AEAKRELDEIRESLQVKQSGWALFKENSNFRRAVFLGVLLQVMQQFTGMNVIMYYAPK
                     IFELAGYTNTTEQMWGTVIVGLTNVLATFIAIGLVDRWGRKPTLTLSFLVMAAGMGVL
                     GTMMHIGIHSPSAQYFAIAMLLMFIVGFAMSAGPLIWVLCSEIQPLKGRDFGITCSTA
                     TNWIANMIVGATFLTMLNTLGNANTFWVYAALNVLFILLTLWLVPETKHVSLEHIERN
                     LMKGRKLREIGAHD"
BASE COUNT         6098 a         6204 c         6468 g         5930 t
ORIGIN      
        1 ggaatgatta tagagccagt ttacaaacag caatgcttcc ggatattgcc cccataacgg
       61 gtggtatgtg gcataataat cttcggcagt atccggaccg cctgaaaagt tatgccatac
      121 agcaagtgga aatggatgca gatttccgca cagtatttct gcgatgtaag tatttgccgc
      181 aaagccgcga cgaaatgcag ttgcagcttt taccagttcc ccattaagaa aatgcagaag
      241 gccgagttca taccagtaag gtgaaaattc atctgcatgt tcctgcagta tatggcgtgc
      301 ctgttcatga gcgccagtgc gtaacagttc cggtcccagt agccagcgtg caccatgatt
      361 atcttcggga ttgtaatcca gtattttttc aatgagcatg atggcatcct gatgacgctg
      421 taaatgtgca tttgccagga ttgccgcata gagggcacga agaaaaggtc ggttgtcagg
      481 atgtatccag atgattctgc cactgaaacc ttcagggata agacgattgc caattgccag
      541 ccctttaagg gcagcattca gcgccttacg cggggcattc tgctccagaa atacgtatgc
      601 caagtgagcg tgtacatcaa taaagtcatt ctcctgtcgg gcaaggcgcc tgagtttgtt
      661 gatgtaactt gtttcgctga tttcatccgc atcgtatgca tcaatcagtt cttcaaactc
      721 atccagcaac gagccaaacc aggtttccgg aaatatgaaa cagccctggt tatcgttcac
      781 ttcaaagcgt aatttgccag tcatattctg aacctgtaaa aaaggatgga ccataacctg
      841 caggctataa aaattgtgat gcctggcatc ggtgtccttt tattgtccgg gattaacgtt
      901 gcccatgata atacagtgaa ttctgttctg tggtaagacg ttgagttatt gaatgagaac
      961 ctcagcctgg ctgatggttc cgccggcata aaaaagaaat tgattgaggc gggtgctctt
     1021 ccccttaaga ggaagagcat gtgtgtaaat ggcgttcagt caccttttca gtggattgtt
     1081 aacttttgca tattcaaacg gactgataca ctcctccctg tttgcatcca gaaaatctgc
     1141 ccaccattgc agcataagcc gacgttcttc cagatgttct gctttatgaa tatacgcggc
     1201 acgtactccg ttgcgctcct gatgactcat ctgacgttca accgcatctc ttgaccataa
     1261 gccggattca atcaatgcac tgcacgccat cgcccggaat ccatggccac agacttcctt
     1321 ggttgtgtca taccccatca ctctgagggc cttatttacg gtattttcac tgattggttt
     1381 acgtgggtcg tgtgctcccg taaagatcag accattttca cctgcccatg tctgaagttc
     1441 tgccagtatg gctacagcct gttggctgag tggcacaaga tgtggtgttc gcatcttgga
     1501 gccccggtat gaaaatttta cgcctggcag aggttcgcgt tcggctggaa tagtccacat
     1561 ggcattgtca atatcaattt cagaccagcg ggcatatctg agttcactgg aacgaataaa
     1621 aaccagtaaa ttcagcataa ctgccagtcg ggtgacaggc tggcctttat aaccgtcaat
     1681 acgcgacaga agttcaggca ggcgtgaaag atccagagca gggcgatgct ggcgttttac
     1741 cgtggtcaat gcaccagcca tatccagagc cgggttatat ctgatgatgc cagactgtac
     1801 ggcataacgc attacagcag atattctttg ctgcagacga ctggcgattt cataaatttg
     1861 tttaacttct gcagcacgaa cagggataag cagatccggg gtactgagtg ttgtgatatc
     1921 ccggttgccg ataaagggga aaacgtgagt ctcgaggctt ttgagtactt tatcgctgtg
     1981 tgattgtgcc cattgtttat tactggcatg ccagcgtcgt gcaatatgtt caaaagagag
     2041 ctgtaaatca gggactactt ttttagctct tttctgttcg caagggtcaa ccccctccga
     2101 aatcagtttt cgggcctcat cccgttttgt acgagcttcg gagagagaaa cttccggata
     2161 cacaccaagt gccagggttt tctccttacc cagaatacga taacggagac gccagtaacg
     2221 ggaaccatta ggatgtacca gcaggaacat gccagccccg tcagtgagtt tataagcctt
     2281 gtcagtgggc tttgcagccc ggatttttgc gtcagtcagt gccatagttc agccttctga
     2341 atgcgttaaa gagggtacaa attctgtcga actggggtat acccgcaatt atacccacac
     2401 aatggggttg atgtgggggg aatctggttg agttcggtag aattgatttg gaggcagaac
     2461 gcttaaattg tggcgttctg aaacgaaaaa cggacctccg tggaggtccg ttgatatgaa
     2521 cttggtgccc ggactcggaa tcgaaccaag gacacgggga ttttcaatcc cctgctctac
     2581 cgactgagct atccgggcaa cggggcgcat taaacctgat tcgcctcgtc tcgtcaatca
     2641 aatttcttca atttactgca gactgcacaa tctatcatca ctttgccgct aatgcacgca
     2701 ttgtcaggca aaccaggcgg tccaggcggc ggaaaggggc attgccagaa ggagtgctgg
     2761 aagcatgttg accaccggga acattttaat gccacagatg cgtaatccgg ttgccagcag
     2821 caataaaccg cctacggcgc tgaagtctgc catcatcgac ggtgtggtca gcggtaatat
     2881 cagcgcggca gcccacgcca gcgttaactg gatgatcagt aatgggatac taatcaccga
     2941 taccgcaata cccagcgagc aggcgaagat catcgccgta aagaaatcaa gaaatgactt
     3001 ggcgattaaa atactcggat cgccggtcat cccttcgttc atcgccccga agatcccggt
     3061 gccgctggcg caaaacagga caataatcgc gacataattc tgaataaaag attcatgcgc
     3121 tggcttctta cgtgagtggc gaaacagatt ttgtgctttg gtgaccgcgg tattgacgcc
     3181 tttttccagc agacaaattt cgccgattaa tgcaccgagt aaggttgcta aaaccatcgc
     3241 cggaaggttg gcgcatttca ccaccagtaa aataccaatc cccagcgatg ccagaccaaa
     3301 aattgatgtc atggagacgc ggatacgttc cggtaagcgt tggctgagca gtgcgccgag
     3361 aacgccaccc agtaagactg cactagcgtt gataaaaggg ccgatgacca caagagttcc
     3421 tgttaactgt attgtttgat tttcttatta tgaacttttt gccttgttga tggcttgtct
     3481 ggtcgcaaag tcctggcttg cacgctttag cgaaaggtgc catgattgcg cgaattttct
     3541 cctctctgta cggagtttgc ccgatgcacg ccacctcctt acattctctc gcttatcgcc
     3601 gtttcgcgcg aaactcctcc ctttttctgc tcatgcggtg aagttaacgc acgctcactg
     3661 caggacaaca gtaaaatcag agcgtttctg cttttactga tgtctggcgg tcggagctgg
     3721 tgaccagttt gacccatatc tcgtggggca gggtttttca ccttgtccgg tattcttact
     3781 tccccgaaac gggttttgcg cttatgaaat caatgaatat tgccgccagt agtgaactgg
     3841 tatcccgact ttctactcat cgccgcgtgg tggcgttggc agatactgat tttacggacg
     3901 tcgcggcagt cgtcattacc gctgcggata gtcgcagtgg cattcttgcg ttgcttaagc
     3961 gcaccggttt tcatctaccg gtgtttttgt attccgaaca tgctgttgaa ttacctgcgg
     4021 gcgttacggc ggtaatcaac ggcaacgagc agcagtggct ggagctggaa tccgcagcct
     4081 gtcagtatga agagaatttg ctgccaccgt tttatgacac gctgacgcag tacgttgaga
     4141 tgggtaatag cacctttgct tgccctggac atcaacatgg tgcgttcttt aaaaagcatc
     4201 ctgccggacg ccatttttac gatttctttg gcgagaacat ctttcgcgcc gatatgtgta
     4261 acgctgacgt aaaattgggc gatctgctta ttcatgaagg atcggcgaaa gatgcgcaga
     4321 aatttgcagc caaagtcttt catgccgata aaacctattt tgtgctgaac ggcacatcgg
     4381 cagcgaataa agtggtgacg aatgcgctgt taacgcgtgg cgatctggtg ctcttcgacc
     4441 gtaacaacca taagtcgaat catcatggcg cgttgattca ggcgggggcg acaccggtct
     4501 atctggaagc ttcacgtaac ccgtttggtt tcattggcgg tattgatgcg cactgtttta
     4561 atgaagagta tctgcgccag caaattcgcg acgttgcgcc agaaaaagcc gacctgccgc
     4621 gcccgtttcg cctggcgatt attcagctgg gaacctatga cggcactgtc tataacgccc
     4681 gtcaggtgat cgataccgtt gggcatctgt gtgattacat tctgtttgat tccgcgtggg
     4741 tcggttacga acagtttatc ccgatgatgg cggatagctc gccgctgctg ttagaactta
     4801 acgaaaacga tccggggatc tttgtgactc agtcggtgca caaacagcag gcgggattct
     4861 cacagacgtc gcagatccat aaaaaagata accatatccg tggacaggcg cgtttttgcc
     4921 cgcataagcg gttgaataat gcctttatgc tccatgcttc taccagcccg ttctatccgc
     4981 tgttcgccgc cctggacgtt aacgccaaaa ttcatgaagg ggagagtggg cgtcggctgt
     5041 gggctgagtg cgttgagttg ggtattgagt cgcgcaaggc tattcttgcg cgctgtaagc
     5101 tgttccgccc gtttatcccg cccgttgttg atggcaaatt gtggcaggat tatccgacgt
     5161 cagtgttagc cagcgaccgc cgttttttca gttttgagcc gggggcgaat tggcacggct
     5221 ttgaaggata tgccgcggat cagtattttg ttgatccgtg caagctgtta ctcaccacgc
     5281 cgggtatcga tgccgaaacc ggcgaatata gcgactttgg cgttccggcg acgattctgg
     5341 cgcactacct gcgtgagaac ggcattgtgc cggagaagtg cgatctcaac tccattctgt
     5401 tcttattaac tccggcggaa agccacgaga agctggcaca actggtggcg atgctggcgc
     5461 agtttgaaca gcatattgag gatgattcgc cgctggctga ggtgttgccg agcgtttata
     5521 acaaatatcc ggtgcgctat cgcgactaca ccctgcgcca gttgtgtcag gagatgcacg
     5581 atttgtatgt cagtttcgac gtcaaagacc tacaaaaagc gatgttccgc cagcagagtt
     5641 tcccgtcagt ggtgatgaat ccccaggatg cgcatagcgc ttatattcgc ggtgaagtgg
     5701 agttggtgcg gattcgtgat gccgaagggc gaattgcggc agaaggggcg ttgccttatc
     5761 cacctggcgt gctttgcgtg gtacccgggg aagtctgggg tggggcggtt caacgttatt
     5821 tccttgcgct ggaagaaggc gtgaatttgt tgccgggatt ttcgccggag ttgcaaggtg
     5881 tctatagcga aaccgatgcg gatggcatga aacggttgta cggttatgtg ttgaagtaag
     5941 aataaaaaaa cgggtcacct tctggcgacc cgtttttctt tgcgtaatta gtggctaacc
     6001 gtctgtgtac ctgtcgggac acgaacgtgt ttatatttga acatcgccat gaacgcgaag
     6061 gccagcacca cggagtaacc tgcgaaaatc agccatacgg tctgccagtc ggtagtgccg
     6121 ttttgggtgt acatctcaac cactttaccg ctcacgatgc cgccgaggat acagccgaag
     6181 ccgttggtca tcatcaggaa catcccctgc gcgctggcgc gaattgccgg gctaacttct
     6241 ttttcgacaa acaccgaacc agagatgttg aagaagtcga atgcgcaacc gtaaacgatc
     6301 atcgacagta ccagcagtac agtaccgaac ggagtcgggt cgccgtaagc aaacagagca
     6361 aaacgcagga tccacgccac aatactgatc atcattacgt tcttaatacc gtagcggctt
     6421 aagaagaacg ggatggtcag aatgaacagg gtttcagaga tctgcgaaat cgacatgatg
     6481 attgacgcat gctgcacgat gaagctgctg gcaaacatcg gatctttgtc aaagctgtgc
     6541 aggaaggtat taccgaacat gttggtaatc tgcagttccg cgcccagcag cattgagaag
     6601 atgaagaaga ttgccatacg cttgttttta aacagcgcga atgcatcgag gcccagcagg
     6661 gttgtccagc tctgattcgc ttgctgttta gcaaccggaa tatgcggcag agtcagggta
     6721 aacagaacca gaatggcgga aagcgctgcg ccaatataca gctgcatgtg gcttaattcg
     6781 aagccggaca ggctcaccac ccacattgcc atgataaagc cgatggtgcc ccagatacgg
     6841 attggcggga agtcagtaac gatatccatc ccggcatttt gcaggcgata gtaagagatg
     6901 gtgttgatta acccaagcgt tggcatataa gcaaacgagt taatcaatat cacaaggaac
     6961 atcgcctccg gcgtagtgac ctctgccgcc atgaacagcg tgatagcgcc aatggtgtgg
     7021 caaatggcat agacccattt cgcacttaac catttgtcgg ccacaatccc cagcagcgca
     7081 ggcataaaga ccgcggcgat acccagtgag ctataaactg cgccgataga agcaccgtca
     7141 aacttcaggg taacaaacat ataggagccg agggtcgtca gccaacttcc ccacagacag
     7201 aactgcagaa aagagaggat tttcagctgc agcttaagat tcatgttaat ttcctcacat
     7261 cgtgatgcgg atgagtgttt gaaaaagcac atttgaccgt gcgagatgat gggattctat
     7321 caacttcgcg gactggtttt ttgttacctg tggcaaataa ttgcaaacat tcccaatttg
     7381 caaaacgaaa ttgtgatgtg gataacattt ttgcccctga gcatcgtcag gggcggttag
     7441 tggaattatc ggcggcggta ggatttttgc gcggtattca ctttataaag ataacggcga
     7501 gattccgcag agggatggcg ggtcgtcagc gtttgataaa catcgcccgg cgtcatggtg
     7561 ttaataatat tggccgcctg aatcttgtca ttcgaaaaga ctcgcagcac gctgcctgca
     7621 ccgccgttat aggcggtgat gacggcataa cgccgcgacg ttgggttatc aattccgccg
     7681 agataaacat tgttcagcat cgccagatac gcggtgccga tatcaatatt gctggcagga
     7741 tcaaacaaga aactgcggct cggcgtgccg gatttcccct gcgagcggaa cacatctttc
     7801 ccggcagtat gttgtaccac ctgcattaat cccagcgcat cggaacggct gaccgcatac
     7861 gggttaaagg atgactcggt ctgcataatc gccagaatca gcgactcatc aacgccatat
     7921 ttccgtgacg cctggcggac catgccgaga tatttgtgcg cacgtttatc aaggtggttt
     7981 ggcaccatgt taatggtgac gctatagatg attcgtagcc cgttgctacg gcttttcaga
     8041 cggtttttca gcagataatc cgcgaagttg ctcgcgcgac cttcccagcg aatcggctgc
     8101 ccggtgttgt ccaccacctg accgtaaagg aaaggttctt tcgaaatcgt aatgtcatca
     8161 acgtcggaat agagatcgac cgaactcgga tcgtcaccca tcagcaacgt tttgataatt
     8221 gcccggcgca gatgcgcggc aggttctgtc ccggcgatgg tttcgatagt aatcgtaccg
     8281 tcatcgaagt tgatgtggct gcgggtctga tattgatcgg tgtatttcac gtagtcctta
     8341 ggaccggcga tcaccacctc tttgaatccc cagatgttct caatattgtg ggcaaattgc
     8401 cccatcagaa tatcaaaacc gttggtgtct ttgacccagg cttcgttata ggtatcgcct
     8461 tttttggtcg tcgaacagga gatgagcaac ggcgcaatca aagccagcgc gagatatttt
     8521 ttcatcattc cgggtgcgtg ttgtgtttgt tttatgcaag ttgccggagg cgtgctccgg
     8581 cactgttttt attttttatc ttccggcgta tagccctcga tatgcacctc tttaccctcg
     8641 aacaggaagt tgaccatctc ctgctccagc agcttgcggt gctcggcatt catcatgttg
     8701 agtttctttt cattaatcag catggtttgt ttgtgctgcc actgcgccca ggcttctttg
     8761 gagatctcgt tatagatgcg ttttcccagc tcgccggggt acagctgaaa atcctgacct
     8821 tctgcttcac gttgcaggaa agtacaaaaa atcgttctgc tcataaatca tcctctttat
     8881 cgactcacgc gctaaaccgg cgcgccagtg cgtaactgct gtaacaaacg ctccacggga
     8941 gccgccaggc caactgacgg cggttgcgct aagttatacc agagcgcatt gccttcatcc
     9001 atgcagccgg tgaatgacga cacgggaagc cacataggca caatatctaa gtggaaatgg
     9061 ctgaaggtat ggcgaaacgc ggtcagctgc gtcaggttat cagcaacaat ctgccgttgc
     9121 gccagccatt gccgcaaact ttcttcgtcg gcaaactgcg ggaaacagta taaaccgccc
     9181 cacaatccgc tcggcggacg ctgcgccagc aatacttcat cttcgtgctg taacagcaga
     9241 aagtagccag tacgctccgg cagcgtctgt ttcggttttt tgcccggata aagcgaccag
     9301 ctattgttag cggtggcaat acatccgttt tgtagcggac agaacgaaca tttcggcttc
     9361 gagcgcgtgc aaatcatcgc gcccaaatcc atcatcgcct gattaaaccg ttccacgcca
     9421 accgcgggcg tcacctgctc gcttaaactc cacagtttat tctcgacctc ttttttccca
     9481 ggccagccgc ttacagcata gcagcgcgcc agcacccgtt tgacgttacc gtcgagaatc
     9541 ggaaagtgct tacccagaga aagcgagaga atcgcgcctg cggtagaacg cccgacgcct
     9601 ggtaacgccg cgacttcttc aaaggtttcc gggaatttac cgctgtgtaa ggtggccact
     9661 tgttgtgccg ctttatgcag attgcgcgcg cgggcgtaat agccaagccc ggtccacaag
     9721 tggagaactt catccagcgg tgcattggcg agatcggtca ccgtcgggaa gcgcgccata
     9781 aagcgttcaa aataggggat aacagtcgca acctgagttt gttgcaacat cacttctgag
     9841 agccatactt tgtagggcgt cttgtcaatt tgccagggca gcgttttccg cccgtattta
     9901 tcgtaccagt ccagaacctg ggctgaaaat tgcgacgctt gcatggtcac cgaattcact
     9961 gttgttgggg gcaagattgc agcacagcga cggcagggtg taaaccggaa ctttccgcag
    10021 ccacggcctt atcatgcttg catccggcaa ctaactttgg ataatgcccg ttttcagaac
    10081 actttcacaa gcgactaaat ctttatgaaa aacgacgtca tctcaccgga atttgatgaa
    10141 aacggccgcc cactgcgccg tatccgtagt tttgtgcgcc gccaggggcg actgacaaaa
    10201 ggccaggaac atgcgctgga aaactactgg ccggtgatgg gcgttgagtt cagcgaagat
    10261 atgctggatt tccccgcgct ttttggccga gaagcgccgg tgacgcttga gattggtttt
    10321 ggcatggggg cgtcgctggt ggcaatggct aaagatcgcc ctgagcagga cttcctcggc
    10381 attgaagtgc attcaccggg cgttggtgcg tgcctggctt ctgcgcatga agagggctta
    10441 agcaacctgc gcgtgatgtg ccacgatgcg gttgaagtgc tgcataaaat gattcctgac
    10501 aattcattgc gcatggtgca gctctttttt cctgacccgt ggcacaaagc gcgccataat
    10561 aaacgccgta tcgttcaggt gccgtttgcc gaactggtaa aaagcaaact gcagctgggg
    10621 ggcgtattcc atatggcgac cgactgggaa ccttatgcgg aacatatgct tgaagtgatg
    10681 tcttcgattg acggttataa aaacctgtca gagagcaatg attacgtgcc gcgtccggca
    10741 tcacgtccgg tgacgaaatt tgaacaacgt ggtcatcgtc ttggtcacgg agtatgggac
    10801 ttaatgttcg agagggtgaa ataatggcaa agaaccgtag ccgtcgtctg cgtaaaaaaa
    10861 tgcacatcga cgaattccag gaattaggat tttcggtggc atggcgattc ccggaaggta
    10921 catcggaaga acagattgat aaaaccgttg atgattttat taacgaggtt atcgaaccga
    10981 acaaactggc ctttgacggc agcggttatc tggcctggga aggtctgatc tgcatgcagg
    11041 aaatcggcaa atgcaccgaa gaacatcagg cgattgtgcg taagtggctg gaagagcgca
    11101 aactggatga ggtacgcacc agcgaacttt tcgacgtttg gtgggactaa gaaagcatac
    11161 gggcgatgac aaatgcaaaa ctgtctgatg cgctacgctt atcaggcctg gaaagatgca
    11221 caatcgagta ggcctgataa ggcgtttacg ccgcatccgg catggatgac atgtactttg
    11281 ttatcaatct ggggccagca aatgctggcc tgatttgttc ttgagggaag actatgatgc
    11341 gcaaaatgct gctggcggca gcactttcag tgacggcgat gaccgctcac gccgactacc
    11401 agtgcagcgt cacgccgcgt gacgatgtga ttgtcagccc gcaaaccgtg caggtgaagg
    11461 gcgaaaacgg caatctggtg atcacgccag acggcaacgt gatgtataac ggtaagcaat
    11521 attccctgaa cgccgcccag cgcgagcagg cgaaggatta tcaggctgaa ctgcgtagca
    11581 cgctgccgtg gattgatgaa ggcgcgaaaa gccgcgtcga gaaagcccgt attgcgctgg
    11641 ataaaattat cgttcaggag atgggcgaaa gcagcaaaat gcgcagccgt ctgaccaaac
    11701 ttgatgcgca gctgaaagag cagatgaacc gcattatcga aacgcgcagc gatggcctga
    11761 cgttccacta taaagccatt gatcaggttc gcgccgaagg ccagcaatta gttaatcagg
    11821 caatgggcgg aattttacag gacagcatta atgaaatggg cgcgaaagcg gtgctgaaaa
    11881 gcggcggtaa cccattacag aatgtgctgg gaagcctggg cggtctgcaa tcctcaatcc
    11941 aaaccgaatg gaaaaagcag gaaaaagatt tccaacagtt tggcaaagat gtttgtagcc
    12001 gcgttgtgac tctggaagat agtcgcaaag ccctggtcgg gaatttaaaa taatcctcta
    12061 ttttaagacg gcataatact tttttatgcc gtttaattct tcgttttgtt acctgcctct
    12121 aactttgtag atctccaaaa tatattcacg ttgtaaattg tttaacgtca aatttcccat
    12181 acaaagctaa gggataatgc gtagcgttca cgtaactgga ggaatgaaat ggagtttttc
    12241 aaaaagacgg cacttgccgc actggttatg ggttttagtg gtgcagcatt ggcattaccc
    12301 aatatcacca ttttagcaac cggcgggacc attgccggtg gtggtgactc cgcaaccaaa
    12361 tctaactaca cagcgggtaa agttggcgta gaaaatctgg ttaatgcggt gccgcaactg
    12421 aaggacattg cgaacgttaa aggcgagcag gtagtgaata tcggctccca ggacatgaac
    12481 gataatgtct ggctgacact ggcgaaaaaa attaacaccg actgcgataa aaccgacggc
    12541 ttcgtcatta cccacggtac cgacacgatg gaagaaaccg cttacttcct cgacctgacg
    12601 gtgaaatgcg acaaaccggt ggtgatggtc ggcgcaatgc gcccgtccac gtccatgagc
    12661 gcagacggtc cattcaacct gtataacgcg gtagtgaccg cagctgataa agcctccgct
    12721 aatcgtggcg tgctggttgt gatgaacgac accgtactgg acggtcgcga tgtcaccaaa
    12781 accaacacca ccgacgtagc gaccttcaag tctgttaact acggtcctct gggatacatt
    12841 cacaacggta agattgacta ccaacgtacc ccggcacgta agcacaccag cgatacgcca
    12901 ttcgatgtct ctaagctgaa tgagctgccg aaagtcggca tcgtttataa ctacgctaac
    12961 gcatccgatc ttccggctaa agcactggta gatgcgggct atgatggcat cgttagcgct
    13021 ggtgtgggta atggtaacct gtataaatct gtgttcgaca cgctggcgac cgccgcgaaa
    13081 aacggcactg cagtcgtgcg ttcttcccgc gtaccgacgg gcgctaccac tcaggatgcc
    13141 gaagtggatg atgcgaaata cggcttcgtc gcctctggca cgctgaaccc gcaaaaagcg
    13201 cgcgtcctgc tgcagctggc tctgacgcaa accaaagatc cgcagcagat ccagcagatc
    13261 ttcaatcagt actaatcacc tcgccccggt atcgtgccgg ggctttttca cttcagactt
    13321 acgtccattg ccaattttca tcaccctaat gataatcacc agaataaatt attccgcgcg
    13381 agggttttcg ggtgaaaaag caatggattg ttggcacggc gctgcttatg ttgatgactg
    13441 ataatgtctg ggcagatggc gaaccgccaa ctgaaaatat cttaaaagat caattcaaaa
    13501 agcagtatca cggcattctc aagcttgatg ctatcacctt aaaaaatctt gatgctaagg
    13561 gtaatcaggc cacctggtca gcggaaggcg atgtctcttc cagtgacgat ctctatacct
    13621 gggtcggtca actggcagat tacgaactgc tcgaacagac ctggacgaaa gataaaccgg
    13681 taaaattttc ggcgatgtta accagtaaag gaacgccagc gtctggctgg tcggtgaact
    13741 tttactcttt tcaggcggca gccagcgatc gtgggcgggt ggtcgacgat atcaaaacga
    13801 ataataaata tctgatcgtg aatagcgaag atttcaatta tcgctttagt cagcttgaat
    13861 cagcgttgaa taaccagaag aactcgattc ctgcgctgga aaaggaagtg aaagcgcttg
    13921 ataagcaaat ggtggcggcg cagaaagcag cggatgcata ttggggaaaa gatgcaaacg
    13981 gtaagcaaat gacccgcgaa gacgccttta aaaaaattca ccaacagcgt gatgagttta
    14041 ataaacagaa cgatagcgag gcgttcgcgg ttaaatatga caaagaggtt tatcaaccgg
    14101 cgattgcggc atgtcataaa cagagtgaag agtgttatga agtgccgatt cagcagaagc
    14161 gagatttcga tataaacgaa caacggcgac agacttttct gcaatcacaa aaacttagcc
    14221 gtaaattgca ggatgactgg ataacgctcg aaaaagggca atatccgtta acgatgaaag
    14281 tttcagaaat aaacagtaaa aaagtcgtga ttttgatgaa aattgacgat atcaaccagg
    14341 ctaatgaacg ctggaaaaaa gataccgaac aattgcggcg caatggggtg attaagtaat
    14401 ttttgcgatg acgcatgagt tgttgccgga tgctgcgtac acgcacgccg catccggcaa
    14461 tacaaattta ctccgccaga aaaagctcca gcaacgaatt taaaaacaac ttcccatgtt
    14521 ccgttatctg ccagtaatct gcacattctg tgagataacc ctgagcgata gcctcttcta
    14581 actgtgggcg aataacttct tcagaaaggc cagtatactg gctaaactcc acgcgcggcg
    14641 cggcttccag cagacggaag cgattcataa agaactcaaa cggtttatct gcagcatcga
    14701 catcacgctg gctttccaga tatcttccct gcataaaacc acgcggatga cgcgttttgg
    14761 tggtacgcag aatgcgacca tccaggaagg tgactttgcc gtgcgcgccg cagccaatac
    14821 cgatgtagtc gccaaatcgc cagtagttga gattgtgctg gcactgataa cctggtttgg
    14881 cgtaagcgga tgtttcatac tgctgatagc ccgctgcggt taataactga tgcccctgtt
    14941 cgaatatatc ccacaacgca tcgtcgtccg gcagcaccgg tgggcgagag ccaaacagcg
    15001 tattaggttc gatggtcagt tgataccagg aaagatgcgg aggattcagt tcaatagcct
    15061 ggcgcagatc gcctagcgcc tcttccagtg attgatccgg cagcccatgc atcaaatcga
    15121 ggttaaagct acgtaaccct aaaccgctcg ccagcttcgc agctcgtttc gcttcttgcg
    15181 ggccatgaat gcgcccaagt cgtttcagct tttcttcgct aaaactctgc acaccaatag
    15241 agatgcggtt cacaccagca cgctgataat cgacaaagcg atcggcttct accgtaccag
    15301 ggttcgcttc catagtaatt tctgcatccg ctgccagcgg caaacgcgca cgcacgccgt
    15361 ccagcagcgt ttgcatcgcc gggccggaaa gcaggctcgg cgtaccaccg ccaataaaaa
    15421 ttgtctttac ttcacggctc tgagcgtaag ccacatcgtt gtccagatcg ttaagcagat
    15481 gctgaacata atcgtcgtgc ggcacttctc ctttcaacgc gtgagagttg aaatcgcagt
    15541 acgggcattt ctgcacgcac cacgggatgt gaatgtagag actcagcggc ggtaatttaa
    15601 ccattacgta aagcgtccag cagcagtttc aatgcctgac cacggtggga aatggcgctc
    15661 ttttcttcgc gggtcagttc ggcagcggtt ttcccttcgg aaggtacgaa gaagattgga
    15721 tcataaccaa agccaccagt gcccgctggt tcacgagtaa tcacgcccgg ccagctaccg
    15781 tggcacacca gcggagtggg atcttccgcg tgacgcagat ataccagcac gcagtggaaa
    15841 cgcgcctgac gttggtcgtc cggtacgtct ttcagtgttt ccagcaattt ttgcagattc
    15901 ttttgatcgg tcgcgtcttc accggaataa cgcgcggagt aaatgcccgg cgctccgcca
    15961 agcacatcta ccgccagacc agagtcgtcg gcaattgccg gtaaaccggt cacttttgcc
    16021 gcatggcgcg ctttcagaat cgcattttcg ataaaggtca ggccggtttc ttcagcggaa
    16081 tctacgccga ggtctgtttg ggccacgata tcaagaccga agtcgctaag cagcgacgcc
    16141 agctcacgca ctttaccggc attgccggtt gcgaggacaa ctttttgcat aggataccta
    16201 attaattaac gccgcgattt ctggcgggat ttgttgcgga ttaatgattt taatttgttt
    16261 gtggcggcca agttcgcctt tttcaatcac cacctggctt ttggcaaccc ggaattgctt
    16321 accgagaaac ttcaccagat gactgttggc ctgaccgtca accggcggcg cggtaatggc
    16381 gactttaact tcgtcgccat gtaaaccgac aatagaatca cggctggctt tcggctgaat
    16441 atagagccgt aaaaccagac cgtcatcatt aactgttacg gcactcataa cgccatccac
    16501 agccccggca gcagcatgtt tccggttgcc tgtaatactt ctgcgacacc catattgatg
    16561 acatacagca gcagaacgag gatcatcggc gagaaatcaa tcccacccat tgccggtagc
    16621 aggcggcgaa tcgggcgcag cagcggatcg gccagctgaa tcagcacgta ttcaatcggg
    16681 ctacgcccct ggcttaccca gctcataatc gccatcacca gcaggaccca gaaaatcagc
    16741 aggccgatgg ttttcagcag aatcaataaa ccggcaatcc agatgattgg caggaaggtc
    16801 accactttaa acagcacgat ggctttgata aaactgagaa tgtacgcaac cagcagtgag
    16861 gcgctgtcaa ttggccccat tgccggaata acgcggcgca gtggcccgat aattggctgc
    16921 gttaccttca ctacaaactg tgagaagggg ttgtaaaaat cacaatgagc ccactgcatc
    16981 cagatgcgta ataacagcac catggtatac agctcaatga ccgttgaaag caggaaagtc
    17041 aacgtattca tggcgttcct ttaattcctt attttttaga gtaatcacgc gcaccaaaaa
    17101 ttgcagtgcc gatacgaacc atcgtgctac ctgccgcaat ggcggcttcc atatcgtccg
    17161 acattccaag agagagcgtg tcgatatgcg ggtagcgcgt tttcagtccg gcaaatgcta
    17221 cagccatttg gcgtgcaact tcaaactgcc ttacatattc tgactcaggc gcagggattg
    17281 ccatcagccc gcgcagacgt aaacgcggta gttcagcgac cgccgctgcc agctcgtcca
    17341 gttcagccag ttgaatcccg gacttactgt tttcatcact aatgttaatt tgaatcagaa
    17401 cgttaagagg gggaagttct gccgggcgct ggtcattgag gcgggtagcg atgcgcaaac
    17461 ggtcgatggt atgacaccag tcgaaatgct ctgccaccag gcggctttta ttagactgca
    17521 acgggccaat aaaatgccat tctaatcctg ttacgcccag ttcctgaaag tggcgaattt
    17581 tatctacccc ttcctgaacg tagttttcac caaattgacg ctgcccggca tcaatggctt
    17641 ctgcgatggc gctcgcaggt tttgttttac tgactgcaag cagcgtaatt tcttctggag
    17701 aacggccgca acgcgttgca gccgctgaga ttttgtcccg gacctgtgcc aggttatgcg
    17761 caatatcgtt cattttccga ggatcttagt atgaatatgg aagaaattgt ggcccttagt
    17821 gtaaagcata acgtctcgga tctacacctg tgcagcgcct ggcccgcacg atggcgcatt
    17881 cgcggcagaa tggaagctgc gccgtttgac gcgccggacg tcgaagagct actgcgggag
    17941 tggctggatg acgatcagcg ggcaatattg ctggagaatg gtcagctgga ttttgctgtg
    18001 tcgctggcgg aaaaccagcg gttgcgtggc agtgcgttcg cgcaacggca aggtatttct
    18061 ctggcattac ggttgttacc ttcgcactgt ccacagctcg aacagcttgg tgcgccaccg
    18121 gtattgccgg aattactcaa gagcgagaat ggcctgattc tggtgacggg ggcgacgggg
    18181 agcggcaaat ctaccacgct ggcggcgatg gttggctatc tcaatcaaca tgccgatgcg
    18241 catattctga cgctggaaga tcctgttgaa tatctctatg ccagccagcg atgtttgatc
    18301 cagcagcggg aaattggttt gcactgtatg acgttcgcat cgggattgcg ggccgcattg
    18361 cgggaagatc ccgatgtgat tttgctcgga gagctgcgtg acagcgagac aatccgtctg
    18421 gcgctgacgg cagcagaaac cggacacctg gtgctggcaa ctttacatac gcgtggtgcg
    18481 gcgcaggcag ttgagcggct ggtggattca tttccggcgc aggaaaaaga tcccgtgcgt
    18541 aatcaactgg caggtagttt acgggcggtg ttgtcacaaa agctggaagt ggataaacag
    18601 gaaggacgcg tggcgctgtt tgaattgctg gttaacacac ccgcggtggg gaatttgatt
    18661 cgcgaaggga aaacccacca gttactgcat gttattcaaa ccgggcagca ggtggggatg
    18721 ttaacgtttc agcagagtta tcagcagcgg gtgggggaag gacgtttgtg acgttgccat
    18781 gcccgatgcg caagcttatc gggcatacaa aggcaataaa tgtctgatgc gacgctaaca
    18841 cgtcttatca gacctacaat cgctgccaca tgtaggccgg ataggcgttc acgccgcatc
    18901 cggcaagatg gcgggatggt acgactatcc gccaggcttt taaatcgcct taatatccct
    18961 gctcgaaata gctttcgaga ataataaccg cagaggcaga gtcgacttta cctttgttga
    19021 gcgcccgata gccgccctgt tcaaacagac cggagcgcgc ttctacggtg ctaagacgct
    19081 cgtcatggag ctttacttca acaccgaaac gaccatgaat acggttggca aatttacgcg
    19141 ctctggcagt caacggctgt tctgtgccgt ccatattcag cggcaaaccg acgatgattt
    19201 cgtccggctg ccactctttc agtaaacgtt cgatgagatt ccagtccggc gtaccgtcct
    19261 gcgctttaat tgcaggcaaa gggcgagcgg tgccggtaat gcgttggccg accgctacgc
    19321 caatgctttt ggtgccgaag tcgaaggcga gtaaggttcc actcatcagg catgccccgc
    19381 cacaccaggc atggtgagaa tatccacacc aatcagtttt gccgcctcgc gccagcggtc
    19441 ggcaatcggc gttttgaaca gaatattcag gtctgccgga gcggttagcc atgcgttgtc
    19501 gagaatctct tgttcaagtt ggcctttctc ccaggaggca tagcccagag ccaccaatat
    19561 gtcagacggt tgtttatcgg tgccgagcgt ttccagcaca tcgcgggaag tggtcatcac
    19621 cgtgttgtct gaaatgcgaa tgctggaagc aaaattggag ggcggagtat gcaaaataaa
    19681 cccgcgatct tcagccagtg ggccgccgag cataaccggt ttatccagac ggattgattc
    19741 atcacgcggc tccggcgtga tcttcagctt ttccagaatc ccttcaattt tgagattttc
    19801 cagcggcttg ttgacgatga tccccattgc accattggta ttatgttcgc aaatgtagac
    19861 cacggaacga cggaaaatcg gatcctggag agcaggcatg gcaataagaa agtgatgctg
    19921 taaattcatt gtcagaggtt ctgtttcctg gttcaaaaaa gcaacagcgc ccagtatgcg
    19981 tggaaagaag ggcgctgtca ctcagagtct caacgagatc cttctcgcta aggtgggtta
    20041 ctgctgctgt aaacgtgctt cgatggcatc cattaacatt ccggtgatcg acaccggaaa
    20101 ctctgcttca atctcacgaa tacaggttgg gctggtgacg ttaatttcag tcagacggtc
    20161 gccaatgata tccagaccaa caaaaatcag ccctttttct ttcagcgtcg gcccgatctg
    20221 acgggcgatt ttccagtcac tttccgtcag cggacgaggt tcaccgcgac caccggcagc
    20281 cagattgcca cgggtttcgc ccccctgcgg aatacgcgcc aggcagtacg gaaccggctc
    20341 gccatccacc accagcacgc gtttgtcgcc atctttaatg gctggcaggt aattttgcgc
    20401 catgcagtag cgagtgccat gctcagtcag ggtttcggca atcacgccga ggtttggatc
    20461 gccttctttc acgcggaaaa tcgacgcgcc gcccataccg tccagcggct taagaatgat
    20521 gtcgctgtgt ttctcccaga acgctttcag ctgtgcttta ttgcgcgtaa ccagcgtttc
    20581 tggcgttaag tcagagaacc aggcggtaaa cagtttctcg ttacagtcgc gcaggctctg
    20641 cggcttgtta acgatcagcg tccctttctc ttcggcacgt tccagaatat aggtcgcgta
    20701 gataaactcg gtatcaaacg gcgggtcttt acgcatcagg atcacatcga gatcggccag
    20761 cggcagatcc tgctcacccg tgaactcgta ccatttatcg tagttctgtt cgacgctcaa
    20821 agtacgggta cgagcgcgag cctcaccatt gatcaaatag agatcggcca tctccatata
    20881 gtgaagttcg taaccacgac gctgtgcttc cagcaacata gcgaaactgg aatctttctt
    20941 gatgttgatg tttgcgatgg ggtccatcac gatgccgagc ttgatcatta ttcttctccg
    21001 ttagcccaaa tcgccaaaac gcacttgtag cgcggtaatg gcggtgagcg cagttgtctc
    21061 agtacgcaaa acgcgaggtc ccaacaggat atcagtaaat tgatagcggg cagtcatggc
    21121 aatttcatct gccgataaac cgccttccgg gccaatcagc aggcggacgc gttcgaccgg
    21181 taacggcaac gtattgatgc tgttactggc gcgcgggtga agattcagtt tcagtccttc
    21241 atcctgctct gcacaccagg cttccagatc catcactgga cggatttccg gcacccggtt
    21301 acggccacac tgctcacagg cagcaattgc aatcttctgc cactgctgga gcttcttgtt
    21361 cagacgttca ctatccagtt taacgccgca gcgctcagaa aaaagtggcg taatgaggct
    21421 tacaccgagt tcgatcgatt tctggatagt aaattccatt ttttcaccac gcgacatcac
    21481 ctgaccgagg tgaatatgca gcggagattc gcgatcgtcg atctggcctt ccagcacctt
    21541 cacttccacg ctttttttgc tggcgctggt aatttcggcg tcaaagacct ggttgctgcc
    21601 gtcaaacaat tgcagcacct gtcccggccc catacgcagt acgcgcccga tatggttggc
    21661 ggcatcttcg caaagcgcga tgtgagaatg gctggtcagt ggttcaggat gataaatgcg
    21721 ggggatacgc atagttaaaa atccgcgtcg tctccccacg cggttgtacg cgtggggtag
    21781 gggttaacaa aaagaatccc gctagtgtag gttagctctt tcgcgcctgg caagcgcgtt
    21841 gcacatacgg gttatgattg ccctgcacct tcgcgatgcg ttcatcacgc tcgcactccc
    21901 agtcggtaac tggatacatc ttgtcccatg cgttgaacag ctgcgtttgc tgacgagaga
    21961 gtgtcaggtt gtattggtca cgcatataga agtaggtgcg cgcaatggca ccgcgtgcac
    22021 gcgctggtgg ttcggcaact ttttctttga aatcgacctt catggcgcat tgaccgtact
    22081 ggccttcacc gccattccac tggctgtaca taaagttgcc gcgatcgcca ttcacctcac
    22141 cgactgacgg ctgcaggtta tgcatatcgc tttccatctt gcgatagacc ggatctttgg
    22201 cgcaattttt acgtccaccg tcctgccagc actggcgctg gtgaccgaat tgccaggcag
    22261 gaacgacgtg ttcccactct acgcggctgg cacggttttc attttttcgc acctgatagc
    22321 cgcacgattg cagatcaaca acgccttttt tgccctgcca gttaatttta catccgcaat
    22381 aaaacgtacc gggcgcgtca gcgtggactt ttaccgccgc ggctttcgct tgagaaaaac
    22441 tattgatacc ttcggccaat gccgggccgg aaaatgctgc gctcagtacc accgcagcaa
    22501 tagacaaata acggtacatc acttactccg tgttaaaacg agccagcaac gtagcgaaag
    22561 ttgttttggt atgcaatcag ccagatcagg aaagttcctg atagttcagg tggttatttc
    22621 gcaaccagct gttcgccgca gtgaacacag cgatagacgg cttcgccacg aactacgcga
    22681 ttatgacggc gtacggtaag ctgatgctcc tggcacttgc agcggtaggg gaaggtgttg
    22741 cggcgcacgg attgcagttc gaactgatgc gtacggcggg cgggaacgcc cagcacgctt
    22801 tccatcatcc acttccactc tttgccatgt ggcgctacgc ggccgaaatg tttccatacc
    22861 agcaaatgtg ccagttcgtg cggcaccact tcttcaataa aggcttcact gttttccaac
    22921 agcaaaacgg gattgaggcg aatttcatag ctttccagcc aggctgttcc ggcagaggtt
    22981 ccgcgctggg tgtaagagag ttttggctcc gggtagttac gccctagctt caggttggcc
    23041 tgggcgagtt tttcccgcag gcgacgcata acggcctgtt ggatggcgat agggagacgg
    23101 gatgttttca tagcggcaga ggatagagcg aagaaaagat gactgcaaga ggtggcttcc
    23161 tccacgacgg gaggaagcct gaggagatta atcgtgagcg ccgatttcgc gcagtttacg
    23221 acctttcatc agattacgtt caatatgttc cagcgaaaca tgtttggttt ccggtaccag
    23281 ccacaatgtc agcaggataa acagtacgtt cagagcagca tacacccaga aggtgttggc
    23341 gttacccagc gtgttgagca tggtcaggaa cgttgcgcca acgatcatgt tggcaatcca
    23401 gttggtggca gtggagcagg tgatgccaaa atcgcggcct ttcagcggct gaatttcgga
    23461 gcacagtacc caaatcagcg gaccagcact catggcaaaa ccgacaataa acatcagcag
    23521 catggcgatg gcgaaatact gcgccgacgg agagtggata ccgatatgca tcattgtacc
    23581 gagtacgccc ataccagcag ccatcaccag gaagctcagc gttagcgttg gtttacgtcc
    23641 ccagcggtca acaaggccga ttgcgataaa ggtggcaagt acgttggtca ggccgacaat
    23701 cactgtcccc cacatttgct cggtggtgtt ggtataaccc gccagttcga agattttcgg
    23761 cgcgtaatac atgatgacgt tcatcccggt gaattgctgc attacctgca atagtacgcc
    23821 aaggaacacc gcgcggcgga agttgctgtt ctctttaaat agcgcccagc cactctgttt
    23881 aacctgcaaa ctttcacgga tttcatccag ttcgcgtttc gcttccgcgc tggtgtcacg
    23941 caggcgtagc agcacgcgtt cggcatcaac aaaacggcgt ttggcggcaa accaacgtgg
    24001 gctgtctggc aggaagaaga caccaatcag cagcaaaatt gccgggatga taatcacacc
    24061 cagcatccag cgccatgcac cggtgtagct gaaggcggta tcagaaagat aagcgccgag
    24121 gatcccgata gtgatcatca actgatacat cgagatcata ctgccgcgaa ttttttccgg
    24181 cgcaatttca gagaggtaca gcggtgcggt ataagaggcc acacccaccg ccagccccag
    24241 cagaacgcgg gaaagaatca gtacttcaac gtttggcgca gccgcagaga acagtgaacc
    24301 ggcaacaaac aggattgcgc cgatcatcag gctctttttg cgcccgagtt taaaggagag
    24361 ccagccgctg cccaccgcac cgactgccgc accgaacatc atggagctta cgacccattc
    24421 ttgcgtgtgc gaagtaatct ggaattcatc tgcaataaac ggcagtgcgc cagcaattac
    24481 accgatatcc aggccaaaga gtaatcccgc cagagcggca aggaagcaga cgaaaaacgt
    24541 cattgccttg tttgaccgcc cctgtttttt agcgtcaggc atgatgccct ccaatatggt
    24601 tattttttat tgtgaattaa gataggtgag tacgacgtaa aaagatgtga agcaaatcac
    24661 atcagtgtaa tcgcttacac taacatttat cttagttatg
//
LOCUS       DAHHTG010000068        23862 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.068, whole genome shotgun sequence.
ACCESSION   DAHHTG010000068 DAHHTG010000000
VERSION     DAHHTG010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 23862)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23862)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23862
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.068"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            19..783
                     /gene="nfeF"
                     /locus_tag="NBI74_002677"
     CDS             19..783
                     /gene="nfeF"
                     /locus_tag="NBI74_002677"
                     /EC_number="1.16.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent ferric chelate reductase"
                     /protein_id="HCD1932988.1"
                     /translation="MNNTPRYPQRVRNDLRFRELTVLRVERISAGFQRIVLGGEALDG
                     FTSRGFDDHSKLFFPQPDAHFVPPTVTEEGIVWPEGSRPPSRDYTPLYDELRHELAID
                     FFIHDGGVASGWAMQAKPGDKLTVAGPRGSLVVPEDYAYQLYVCDESGMPALRRRLET
                     LSKLAVKPQVSALVSVRDNACQDYLAHLDGFNIEWLAHDEQAVDARLAQMQIPADDYF
                     IWITGEGKVVKNLSRRFETEQYDPQRVRAAAYWHAK"
     gene            complement(837..912)
                     /locus_tag="NBI74_002678"
     tRNA            complement(837..912)
                     /locus_tag="NBI74_002678"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(877..879),aa:Ile,seq:cat)
     gene            1037..1543
                     /gene="mug"
                     /locus_tag="NBI74_002679"
     CDS             1037..1543
                     /gene="mug"
                     /locus_tag="NBI74_002679"
                     /EC_number="3.2.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G/U mismatch-specific DNA glycosylase"
                     /protein_id="HCD1932989.1"
                     /translation="MVEDILAPGLRVVFCGINPGLSSAGTGFPFAHPANRFWKVIYQA
                     GFTDRQLKPQEAQHLLDYRCGVTKLVDRPTVQANEVSKQELHAGGRKLIEKIEDYQPQ
                     ALAILGKQAYEQGFSQRGAQWGKQTLTIGSTQIWVLPNPSGLSRVSLEKLVEAYRELD
                     QALVVRGR"
     gene            complement(1621..3462)
                     /gene="rpoD"
                     /locus_tag="NBI74_002680"
     CDS             complement(1621..3462)
                     /gene="rpoD"
                     /locus_tag="NBI74_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HCD1932990.1"
                     /translation="MEQNPQSQLKLLVTRGKEQGYLTYAEVNDHLPEDIVDSDQIEDI
                     IQMINDMGIQVMEEAPDADDLMLAENTADEDAAEAAAQVLSSVESEIGRTTDPVRMYM
                     REMGTVELLTREGEIDIAKRIEDGINQVQCSVAEYPEAITYLLEQYDRVEAEEARLSD
                     LITGFVDPNAEEDLAPTATHVGSELSQEDLDDDEDEDEEDGDDDTADDDNSIDPELAR
                     EKFAELRAQYIVTRDTIKAKGRSHAAAQEEILKLSEVFKQFRLVPKQFDYLVNSMRVM
                     MDRVRTQERLIMKLCVEQCKMPKKNFITLFTGNETSDTWFNAAIAMNKPWSEKLHDVS
                     EEVHRALQKLQQIEEETGLTIEQVKDINRRMSIGEAKARRAKKEMVEANLRLVISIAK
                     KYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAITRSIADQARTI
                     RIPVHMIETINKLNRISRQMLQEMGREPTPEELAERMLMPEDKIRKVLKIAKEPISME
                     TPIGDDEDSHLGDFIEDTTLELPLDSATTESLRAATHDVLAGLTAREAKVLRMRFGID
                     MNTDHTLEEVGKQFDVTRERIRQIEAKALRKLRHPSRSEVLRSFLDD"
     gene            complement(3657..5402)
                     /gene="dnaG"
                     /locus_tag="NBI74_002681"
     CDS             complement(3657..5402)
                     /gene="dnaG"
                     /locus_tag="NBI74_002681"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HCD1932991.1"
                     /translation="MAGRIPRVFINDLLARTDIVDLIDARVKLKKQGKNFHACCPFHN
                     EKTPSFTVNGEKQFYHCFGCGAHGNAIDFLMNYDKLEFVETVEELAAMHNLEVPFEAG
                     SGPSQIERHQRQTLYQLMDGLNTFYQQSLQQPVAMSARQYLEKRGLSHEVIARFAIGF
                     APPGWDNVLKRFGGNPENRQSLIDAGMLVTNDQGRSYDRFRERVMFPIRDKRGRVIGF
                     GGRVLGNDTPKYLNSPETDIFHKGRQLYGLYEAQQDNAEPNRLLVVEGYMDVVALAQY
                     GINYAVASLGTSTTADHIQLLFRATNNVICCYDGDRAGRDAAWRALETALPYMTDGRQ
                     LRFMFLPDGEDPDTLVRKEGKEAFEARMEQAMPLSAFLFNSLMPQVDLSTPDGRARLS
                     TLALPLISQVPGETLRIYLRQELGNKLGILDDSQLERLMPKAAESGVSRPVPQLKRTT
                     MRILIGLLVQNPELATLVPPLENLDENKLPGLGLFRELVNTCLSQPGLTTGQLLEHYR
                     GTNNAATLEKLSMWDDIADKNIAEQTFTDSLNHMFDSLLELRQEELIARERTHGLSNE
                     ERLELWTLNQELAKK"
     gene            complement(5513..5728)
                     /gene="rpsU"
                     /locus_tag="NBI74_002682"
     CDS             complement(5513..5728)
                     /gene="rpsU"
                     /locus_tag="NBI74_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HCD1932992.1"
                     /translation="MPVIKVRENEPFDVALRRFKRSCEKAGVLAEVRRREFYEKPTTE
                     RKRAKASAVKRHAKKLARENARRTRLY"
     gene            5966..6979
                     /gene="tsaD"
                     /locus_tag="NBI74_002683"
     CDS             5966..6979
                     /gene="tsaD"
                     /locus_tag="NBI74_002683"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001264383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HCD1932993.1"
                     /translation="MRVLGIETSCDETGIAIYDDEKGLLANQLYSQVKLHADYGGVVP
                     ELASRDHVRKTVPLIQEALKESGLTAKDIDAVAYTAGPGLVGALLVGATVGRSLAFAW
                     DVPAIPVHHMEGHLLAPMLEDNPPEFPFVALLVSGGHTQLISVTGIGQYELLGESIDD
                     AAGEAFDKTAKLLGLDYPGGPLLSKMAAQGTAGRFVFPRPMTDRPGLDFSFSGLKTFA
                     ANTIRDNGTDDQTRADIARAFEDAVVDTLMIKCKRALDQTGFKRLVMAGGVSANRTLR
                     AKLAEMMKKRRGEVFYARPEFCTDNGAMIAYAGMVRFKAGATADLGVSVRPRWPLAEL
                     PAA"
     gene            complement(7022..8485)
                     /gene="ttdT"
                     /locus_tag="NBI74_002684"
     CDS             complement(7022..8485)
                     /gene="ttdT"
                     /locus_tag="NBI74_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-tartrate/succinate antiporter"
                     /protein_id="HCD1932994.1"
                     /translation="MKPSTEWWRYLAPLAVIAIIALLPLPAGLESHTWLYFAVFTGVI
                     VGLILEPVPGAVVAMVGISIIAILSPWLLFSPEQLAQPGFKFTAKSLSWAVSGFSNSV
                     IWLIFAAFMFGTGYEKTGLGRRIALILVKKMGHRTLFLGYAVMFSELILAPVTPSNSA
                     RGAGIIYPIIRNLPPLYQSQPNDSSSRSIGSYIMWMGIVADCVTSAIFLTAMAPNLLL
                     IGLMKSASHAMLSWGDWFLGMLPLSILLVLLVPWLAYVLYPPVLKSGDQVPRWAETEL
                     QAMGPLCSREKRMLGLMVGALVLWIFGGDYIDAAMVGYSVVALMLLLRIISWDDIVSN
                     KAAWNVFFWLASLITLATGLNNTGFISWFGKLLAGSLSGYSPTIVMVALIVVFYLLRY
                     FFASATAYTSALAPMMIAAALAMPEIPLPVFCLMVGAAIGLGSILTPYATGPSPIYYG
                     SGYLPTADYWRLGAIFGLIFLVLLVITGLLWMPVVLL"
     gene            complement(8533..9138)
                     /gene="ttdB"
                     /locus_tag="NBI74_002685"
     CDS             complement(8533..9138)
                     /gene="ttdB"
                     /locus_tag="NBI74_002685"
                     /EC_number="4.2.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit beta"
                     /protein_id="HCD1932995.1"
                     /translation="MKKILTTPIKAEDLQDIRVGDVIYLTGTLVTCRDVCHRRLIELK
                     RPIPYDLNGKAIFHAGPIVRKNGDKWEMVSVGPTTSMRMESFEREFIEQTGVKLVVGK
                     GGMGPLTEEGCQKFKALHVIFPAGCAVLAATQVEEIEEVHWTELGMPESLWVCRVKEF
                     GPLIVSIDTHGNNLIAENKKLFAERRDPIVEEICEHVHYIK"
     gene            complement(9135..10046)
                     /gene="ttdA"
                     /locus_tag="NBI74_002686"
     CDS             complement(9135..10046)
                     /gene="ttdA"
                     /locus_tag="NBI74_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit alpha"
                     /protein_id="HCD1932996.1"
                     /translation="MMSESNKQQAVNKLTEIVANFTAMISTRMPDDVVDKLKQLKDAE
                     TSSMGKIIYHTMFDNMQKAIDLNRPACQDTGEIMFFVKVGSRFPLLGELQSILKQAVE
                     EATVKAPLRHNAVEIFDEVNTGKNTGSGVPWVTWDIVPDGDDAEIEVYMAGGGCTLPG
                     RSKVLMPSEGYEGVVKFVFENISTLAVNACPPVLVGVGIATSVETAAVLSRKAILRPI
                     GSRHPNPKAAELELRLEEGLNRLGIGPQGLTGNSSVMGVHIESAARHPSTIGVAVSTG
                     CWAHRRGTLLVHADLTFENLSHTRSAL"
     gene            10253..11185
                     /gene="ttdR"
                     /locus_tag="NBI74_002687"
                     /gene_synonym="dan"
     CDS             10253..11185
                     /gene="ttdR"
                     /locus_tag="NBI74_002687"
                     /gene_synonym="dan"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator TtdR"
                     /protein_id="HCD1932997.1"
                     /translation="MLNSWPLAKDLQVLVEIVHSGSFSAAAATLGQTPAFVTKRIQIL
                     ENTLATTLLNRSARGVALTESGQRCYEHALEILTQYQRLVDDVTQIKTRPEGMIRIGC
                     SFGFGRSHIAPAITELMRNYPELQVHFELFDRQIDLVQDNIDLDIRINDAIPDYYIAH
                     LLTKNKRILCAAPEYLQKYPQPQSLQELSRHDCLVTKERDMTHGIWELGNGQEKKSVK
                     VSGHLSSNSGEIVLQWALEGKGIMLRSEWDVLPFLESGKLVRVLPEYAQSANIWAVYR
                     EPLYRSMKLRVCVEFLAAWCQQRLGKPDEGYQVM"
     gene            complement(11198..11815)
                     /gene="plsY"
                     /locus_tag="NBI74_002688"
     CDS             complement(11198..11815)
                     /gene="plsY"
                     /locus_tag="NBI74_002688"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HCD1932998.1"
                     /translation="MSAIAPGMILIAYLCGSISSAILVCRLCGLPDPRTSGSGNPGAT
                     NVLRIGGKGAAVAVLIFDVLKGMLPVWGAYELGVSPFWLGLIAIAACLGHIWPVFFGF
                     KGGKGVATAFGAIAPIGWDLTGVMAGTWLLTVLLSGYSSLGAIVSALIAPFYVWWFKP
                     QFTFPVSMLSCLILLRHHDNIQRLWRRQETKIWTKFKRKREKDPE"
     gene            11920..12288
                     /gene="folB"
                     /locus_tag="NBI74_002689"
     CDS             11920..12288
                     /gene="folB"
                     /locus_tag="NBI74_002689"
                     /EC_number="4.1.2.25"
                     /EC_number="5.1.99.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional dihydroneopterin
                     aldolase/7,8-dihydroneopterin epimerase"
                     /protein_id="HCD1932999.1"
                     /translation="MDIVFIEQLSVITTIGVYDWEQTIEQKLVFDIEMAWDNRKAAKS
                     DDVADCLSYADIAETVVSHVEGARFALVERVAEEVAELLLARFNSPWVRIKLSKPGAV
                     ARAANVGVIIERGNNLKENN"
     gene            12379..13200
                     /gene="bacA"
                     /locus_tag="NBI74_002690"
     CDS             12379..13200
                     /gene="bacA"
                     /locus_tag="NBI74_002690"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HCD1933000.1"
                     /translation="MSDMHSLLIAAILGVVEGLTEFLPVSSTGHMIIVGHLLGFEGDT
                     AKTFEVVIQLGSILAVVVMFWRRLFGLIGIHFGRPLQHEGENKGRLTLIHILLGMIPA
                     VVLGLLFHDTIKSLFNPINVMYALVVGGLLLIAAECLKPKEPRAPGLDDMTYRQAFMI
                     GCFQCLALWPGFSRSGATISGGMLMGVSRYAASEFSFLLAVPMMMGATALDLYKSWGF
                     LTTGDIPMFAVGFITAFVVALIAIKTFLQLIKRISFIPFAIYRFIVAAAVYVVFF"
     gene            complement(13280..14518)
                     /gene="cca"
                     /locus_tag="NBI74_002691"
     CDS             complement(13280..14518)
                     /gene="cca"
                     /locus_tag="NBI74_002691"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused tRNA nucleotidyltransferase/2',3'-cyclic
                     phosphodiesterase/2' nucleotidase/phosphatase Cca"
                     /protein_id="HCD1933001.1"
                     /translation="MKIYLVGGAVRDALLGLPVKDRDWVVVGSTPQEMLDAGYQQVGR
                     DFPVFLHPQTHEEYALARTERKSGSGYTGFTCYAAPDVSLEDDLKRRDLTINALAQDD
                     NGEIIDPYNGLGDLQNRLLRHVSPAFGEDPLRVLRVARFAARYAHLSFRIADETLALM
                     REMTHAGELEHLTPERVWKETESALTTRNPQVFFQVLRDCGALRVLFPEIDALFGVPA
                     PARWHPEIDTGIHTLMTLSMAAMLSPQVDVRFATLCHDLGKGLTPPELWPRHHGHGPA
                     GVKLVEQLCQRLRVPNEIRDLARLVAEFHDLIHTFPMLNPKTIVKLFDSIDAWRKPQR
                     VEQLALTSEADVRGRTGFESADYPQGRWLREAWEVAQSVPTKAVVEAGFKGVEIREEL
                     TRRRIAAVASWKEQRCPKPD"
     gene            complement(14582..15202)
                     /locus_tag="NBI74_002692"
     CDS             complement(14582..15202)
                     /locus_tag="NBI74_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HCD1933002.1"
                     /translation="MPKLRLIGLTLLALSATAVSHAEETRYVSDELNTWVRSGPGDHY
                     RLVGTVNAGEEVTLLQTDANTNYAQVKDSSGRTAWIPLKQLSTEPSLRSRVPDLENQV
                     KTLTDKLTNIDNTWNQRTAEMQQKVAQSDSVINRLKEENQKLKNELIVAQKKVDAASV
                     QLDDKQRTIIMQWFMYGGGVLGLGLLLGLVLPHLIPSRKRKDRWMN"
     gene            15444..16745
                     /gene="ygiF"
                     /locus_tag="NBI74_002693"
     CDS             15444..16745
                     /gene="ygiF"
                     /locus_tag="NBI74_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic triphosphatase"
                     /protein_id="HCD1933003.1"
                     /translation="MAQEIELKFIVNHSAVEALRDHLNTLGGEHHDPVQLLNIYYETP
                     DNWLRGHDMGLRIRGENGRYEMTMKVAGRVTGGLHQRPEYNVALSEPTLDLAQLPTEV
                     WPNGELPTDLASRVQPLFSTDFYREKWLVEVDGSRIEIALDQGEVKAGEFAEPICELE
                     LELLSGDMRAVLKLANQLVSQTGLRQGSLSKAARGYHLAQGNPAREIKPTTILHVAAK
                     ADVEQGLEAALELALAQWQYHEELWVRGNDAAKEQVLAAIGLVRHTLMLFGGIVPRKA
                     STHLRDLLTQCEATIASAVSAVTAVYSTETAMAKLALTEWLVSKAWQPFLDAKAQSKM
                     SDSFKRFADIHLSRHAAELKSVFCQPLGDRYRDQLPRLTRDIDSILLLAGYYDPVVAQ
                     DWLENWQGLRHAIATGQRIEIEHFRNEANNQEPFWLHSGKR"
     gene            16768..19608
                     /gene="glnE"
                     /locus_tag="NBI74_002694"
     CDS             16768..19608
                     /gene="glnE"
                     /locus_tag="NBI74_002694"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HCD1933004.1"
                     /translation="MKPLSSPLQQYWQTVVERLPEPLAEESLSAQAKSVLTFSDFVQD
                     SVIAHPEWLTELESQPPQADEWQHYAAWLQEALSNVSDEAGLMRELRLFRRRIMVRIA
                     WAQTLALVTEESILQQLSHLAETLIVAARDWLYDACCREWGTPCNAQGEAQPLLILGM
                     GKLGGGELNFSSDIDLIFAWPEHGCTQGGRRELDNAQFFTRMGQRLIKVLDQPTQDGF
                     VYRVDMRLRPFGESGPLVLSFAALEDYYQEQGRDWERYAMVKARIMGDSDGVYANELR
                     ALLRPFVFRRYIDFSVIQSLRNMKGMIAREVRRRGLTDNIKLGAGGIREIEFIVQVFQ
                     LIRGGREPSLQSRSLLPTLSAIAALHLLSENDAEQLRVAYLFLRRLENLLQSINDEQT
                     QTLPSDELNRARLAWAMDFADWPQLTGVLTAHMANVRRVFNELIGDDESETQEESLSE
                     QWRELWQDALQEDDTTPVLAHLSEDDRKQVLMLIADFRKELDKRTIGPRGRQVLDHLM
                     PHLLSDVCAREDAAVTLSRITALLVGIVTRTTYLELLSEFPAALKHLISLCAASPMIA
                     SQLARYPLLLDELLDPNTLYQPTATDAYRDELRQYLLRVPEDDEEQQLEALRQFKQAQ
                     LLRIAAADIAGTLPVMKVSDHLTWLAEAMIDAVVQQAWVQMVARYGKPNHLNEREGRG
                     FAVVGYGKLGGWELGYSSDLDLIFLHDCPMDAMTDGEREIDGRQFYLRLAQRIMHLFS
                     TRTSSGILYEVDARLRPSGAAGMLVTSAEAFADYQKNEAWTWEHQALVRARVVYGDPQ
                     LTAHFDAVRREIMTLPREGKTLQTEVREMREKMRAHLGNKHRDRFDIKADEGGITDIE
                     FITQYLVLRYAHEKPKLTRWSDNVRILELLAQNDIMEEQEAMALTRAYTTLRDELHHL
                     ALQELPGHVSEDCFTAERELVRASWQKWLVEE"
     gene            19656..21089
                     /gene="hldE"
                     /locus_tag="NBI74_002695"
     CDS             19656..21089
                     /gene="hldE"
                     /locus_tag="NBI74_002695"
                     /EC_number="2.7.1.167"
                     /EC_number="2.7.7.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase/D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase HldE"
                     /protein_id="HCD1933005.1"
                     /translation="MKVTLPEFERAGVMVVGDVMLDRYWYGPTSRISPEAPVPVVKVN
                     TIEERPGGAANVAMNIASLGANARLVGLTGIDDAARALSKSLADVNVKCDFVSVPTHP
                     TITKLRVLSRNQQLIRLDFEEGFEGVDPQPLHERINQALSSIGALVLSDYAKGALASV
                     QQMIQLARKAGVPVLIDPKGTDFERYRGATLLTPNLSEFEAVVGKCKTEEEIVERGMK
                     LIADYELSALLVTRSEQGMSLLQPGKAPLHMPTQAQEVYDVTGAGDTVIGVLAATLAA
                     GNSLEEACFFANAAAGVVVGKLGTSTVSPIELENAVRGRADTGFGVMTEEELKLAVAA
                     ARKRGEKVVMTNGVFDILHAGHVSYLANARKLGDRLIVAVNSDASTKRLKGDSRPVNP
                     LEQRMIVLGALEAVDWVVSFEEDTPQRLIAGILPDLLVKGGDYKPEEIAGSKEVWANG
                     GEVLVLNFEDGCSTTNIIKKIQLDKKG"
     gene            complement(21209..21268)
                     /gene="ibsE"
                     /locus_tag="NBI74_002696"
     CDS             complement(21209..21268)
                     /gene="ibsE"
                     /locus_tag="NBI74_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsE"
                     /protein_id="HCD1933006.1"
                     /translation="MMKFVIILVVLLLLSFPTY"
     gene            complement(21508..23169)
                     /locus_tag="NBI74_002697"
     CDS             complement(21508..23169)
                     /locus_tag="NBI74_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HCD1933007.1"
                     /translation="MDDIVNSVPSWMFTAIIAVCILFIIGIIFARLYRRASAEQAFVR
                     TGLSGQKVVMSGGAIVMPIFHEIIPINMNTLKLEVSRSTIDSLITKDRMRVDVVVAFF
                     VRVKPSVEGIATAAQTLGQRTLSPEDLRMLVEDKFVDALRATAAQMTMHELQDTRENF
                     VQGVQNTVAEDLSKNGLELESVSLTNFNQTSKEHFNPNNAFDAEGLTKLTQETERRRR
                     ERNEVEQDVEVAVREKNRDALSRKLEIEQQEAFMTLEQEQQVKTRTAEQNAKIAAFEA
                     ERRREAEQTRILAERQIQETEIDREQAVRSRKVEAEREVRIKEIEQQQVTEIANQTKS
                     IAIAAKSEQQSQAEARANLALAEAVSAQQNVETTRQTAEADRAKQVALIAAAQDAETK
                     AVELTVRAKAEKEAAEMQAAAIVELAEATRKKGLAEAEAQRALNDAINVLSDEQTSLK
                     FKLALLQALPAVIEKSVEPMKSIDGIKIIQVDGLNRGGATGDANTGNVGGGNLAEQAL
                     SAALSYRTQAPLIDSLLNEIGVSGGSLAALTSSLSSTTPVEEKAE"
     gene            complement(23196..23825)
                     /locus_tag="NBI74_002698"
     CDS             complement(23196..23825)
                     /locus_tag="NBI74_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1449 family protein"
                     /protein_id="HCD1933008.1"
                     /translation="MILFADYNTPYLFAISFVLLIGLLEIFALICGHMLSGALDAHLD
                     HYDSITTGHISQALHYLNIGRLPALVVLCLLAGFFGLIGILLQHACVTLWQSPLPNLF
                     VVPVSLLFTIIAVHYTGKVVAPWIPRDHSSAITEEEYVGSMALITGHQATSGNPCEGK
                     LTDQFGQIHYLLLEPEEGKTFTKGDKVLIICRLSATRYLAENNPWPQIL"
BASE COUNT         5512 a         6059 c         6538 g         5753 t
ORIGIN      
        1 atcacgaagg cgataacaat gaataacacc ccccgctacc cacagcgcgt tcgcaatgat
       61 ctgcgcttcc gtgaactgac tgtgctacgc gttgagcgca tcagcgccgg ttttcagcgc
      121 attgtcctcg gcggcgaggc gctggacggt tttacatcgc gcggctttga cgatcacagc
      181 aaactcttct ttcctcaacc tgacgctcac tttgtgccgc caacggtaac ggaagagggc
      241 atcgtctggc cggaaggatc acgcccaccg tcgcgtgact atacgccgct gtatgacgaa
      301 ctacgccatg aactggcgat tgattttttt attcacgacg gtggggtcgc cagcggctgg
      361 gcgatgcagg cgaaaccggg cgataaactt acggtggcag gtccgcgcgg ttcgctggtg
      421 gtgccggaag attacgcgta tcagctgtat gtctgcgatg aatccggaat gcccgcactg
      481 cgccgccgcc tggaaacgtt gagcaaactt gctgttaaac cgcaagttag tgcgctggtt
      541 agcgtgcggg ataacgcctg tcaggattat ctcgcgcacc ttgatggttt taatatcgaa
      601 tggctggcac atgatgagca ggcggtagat gcgcgtctgg cgcagatgca aatccctgcc
      661 gatgattact tcatctggat aaccggcgaa ggcaaagtcg ttaagaattt aagccgccgc
      721 tttgaaacgg aacagtatga cccacagcgg gtacgtgcgg cggcttactg gcacgcaaag
      781 taacggatat ataaaaagaa aaaggctgac gatttctcgt cagcctttgc tatgtctggt
      841 ggcccctgct ggacttgaac cagcgaccaa gcgattatga gtcgcctgct ctaaccactg
      901 agctaagggg ccatggtagc ggattataaa gtaactccgt gtcgcaatcc agccattacc
      961 gcgcgcctgc tgtttttata aacaatgtat tttcaatccg ttatactttt cctggtgatg
     1021 ttagaaagga gtaaatatgg ttgaggatat tttggctcca gggttacggg tcgtgttttg
     1081 cggtatcaac cctggtcttt catccgccgg gactggtttt ccctttgctc atccggcaaa
     1141 tcgcttctgg aaggtgatat atcaggccgg gtttaccgac cgtcagttga agccgcagga
     1201 ggcacagcat ctgctggatt atcgttgtgg cgtcaccaaa ctggtagacc gtccaacggt
     1261 gcaagccaat gaagtttcaa agcaggagct acacgcaggc gggcgtaagc tgattgaaaa
     1321 aattgaagat tatcagccgc aggcgttggc gattctgggc aaacaagcat atgaacaggg
     1381 attcagccag cgcggtgcac agtgggggaa acaaacgctc accattggtt cgacgcagat
     1441 ttgggtgctg ccaaatccca gcggtttaag tcgcgtttca ctggagaaac tggttgaagc
     1501 gtatcgcgag ctggaccagg cgctggtggt gcgtgggcga taaaaacgcc accgaaaagg
     1561 tggcgtttgt gcggagggac tagtgccggg tgcggcgtaa cacctgatcc ggcctaccga
     1621 ttaatcgtcc aggaagctac gcagcacttc agaacggctc gggtgacgca gtttgcgcag
     1681 cgccttcgct tcgatctgac ggatacgttc gcgggtaacg tcgaactgtt tacccacttc
     1741 ttccagcgtg tggtcggtgt tcatatcgat accgaaacgc atacgcagaa ctttcgcttc
     1801 acgcgcggtc aggccagcca gcacgtcgtg cgttgccgca cgcaggcttt cggtggtcgc
     1861 agaatccagc ggcagctcga gggtggtatc ctcgatgaaa tcccccagat gcgaatcttc
     1921 atcatcaccg atcggcgttt ccatggagat tggctctttg gcgatcttca gcactttgcg
     1981 gatcttgtct tccggcatca gcatgcgttc agccagttct tccggcgttg gttcacggcc
     2041 catctcttgc agcatctggc gagaaatacg gttgagcttg ttgatagtct caatcatatg
     2101 caccggaata cggatggtgc gcgcctgatc cgcgatagag cgggtgatcg cctgacggat
     2161 ccaccaggta gcgtaggtgg agaacttgta accacggcgg tattcgaatt tatcaaccgc
     2221 tttcatcagg ccgatgttac cttcctgaat caggtcaagg aactgcaagc cacggttggt
     2281 gtatttcttg gcgatagaaa taaccagacg taagttcgct tcaaccatct ctttcttcgc
     2341 acggcgggct ttcgcttcac cgatggacat acgacggttg atatctttaa cctgctcgat
     2401 ggtcaggccg gtttcttctt caatctgctg cagtttctgc agggcgcgat gcacttcttc
     2461 agagacatca tgcagttttt ccgaccacgg cttgttcatc gcaattgccg cgttgaacca
     2521 ggtatcgctg gtttcgttgc cggtaaacag ggtaatgaag tttttcttcg gcattttgca
     2581 ctgctcaacg cagagcttca tgatcagacg ttcttgcgta cgaacgcggt ccatcatgac
     2641 gcgcatgctg ttgaccaggt agtcaaactg cttcggcacc aggcggaact gtttgaatac
     2701 ttcagacagt ttcaggatct cttcctgagc ggcagcgtga ctgcggcctt tcgctttgat
     2761 ggtgtcacgc gttacaatgt actgagcgcg cagttccgca aatttttcgc gagccagttc
     2821 cgggtcgatg ctgttgtcat catcggcggt atcgtcgtca ccatcttctt cgtcttcatc
     2881 ttcgtcatcg tccagatctt cctgggaaag ctcagaaccg acgtgagtgg cggtaggtgc
     2941 cagatcttct tctgcgttcg ggtcaacaaa gccggtgatc agatcggaca gacgcgcttc
     3001 ttctgcttca acacgatcgt actgttccag cagataggtg atcgcttccg gatattcagc
     3061 aacggagcat tgaacctggt tgatcccgtc ttcaatacgc ttagcgatgt caatttcgcc
     3121 ttcgcgggtc aacagttcaa cggtgcccat ttcacgcatg tacatgcgta ccgggtcagt
     3181 cgtgcgcccg atttcagatt ccacgctgga aagcacctgc gcggcggctt cggcagcatc
     3241 ttcgtccgcg gtgttttcag ccagcatcag atcatcggca tccggtgctt cttccatcac
     3301 ctgaatgccc atgtcgttga tcatttggat gatgtcttcg atctgatctg aatcgacgat
     3361 atcttccggc agatggtcat tgacctcggc ataggtcaga tagccttgct ccttaccacg
     3421 ggtgacaaga agtttcagct gtgactgcgg gttttgctcc ataagacggt atccacactt
     3481 atttcatgag gttggtgttg ggcgattgac ccgatgtcct tcagccgtta acgctgaaat
     3541 ccaacggttg tttaccgcca acgataatta cgagggcgta cttatatatt tgccgctgcc
     3601 tctcagtgcg gctgtcgggg gcttcccgat cgctcttcgg cacttaagcc gttaaatcac
     3661 tttttcgcca gctcctggtt taatgtccag agctccaggc gttcttcgtt gcttaaacca
     3721 tgcgtgcgct cacgagcgat taactcttcc tggcgcagtt caagcagcga atcaaacata
     3781 tggttgagtg agtcggtgaa ggtttgctca gcaatattct tatctgctat atcgtcccac
     3841 atcgacagtt tttcaagggt ggcagcatta tttgtaccac gatagtgctc taaaagttgc
     3901 ccggtggtca gacctggctg ggagagacaa gtgttgacca gttctctgaa taagccaagt
     3961 ccagggagct tattttcatc cagattctca agcggcggga ccaacgtcgc taattctgga
     4021 ttttgcacca gcaaccctat aagtatacgc atggtcgtgc gttttagctg cggaacaggg
     4081 cgagaaacgc cgctctctgc cgcttttggc attaatcgtt caagctggct gtcatcaagt
     4141 atgcctaatt tgttgcctaa ttcctgacga agatatattc gcagcgtttc gcccggcact
     4201 tgcgatatca gtggcagtgc cagcgtactc aaacgtgcgc gcccgtcagg ggtactcaga
     4261 tcaacttgcg gcatcagact gttaaacaga aatgcggaga gtggcatcgc ctgctccatc
     4321 cgcgcttcaa acgcttcttt accttctttt cgtaccagcg tgtcagggtc ttcgccatcc
     4381 ggcaaaaaca taaagcgtag ctgacgaccg tctgtcatgt aaggcaacgc cgtttccagc
     4441 gctcgccagg cggcatcgcg gcctgcacgg tcgccgtcat aacagcaaat gacattgttg
     4501 gtcgcgcgga acaacagttg tatgtgatcg gcggtggttg acgtacctaa cgacgcaacg
     4561 gcgtaattaa tgccgtattg cgccagcgcc accacgtcca tatagccttc gaccacaagc
     4621 agacgattgg gttcagcgtt atcctgctgc gcttcataaa gaccgtaaag ctggcggcct
     4681 ttatggaaaa tgtccgtttc cggcgagttc aggtatttgg gggtatcgtt gcccagcacg
     4741 cgtccgccaa aaccaatcac ccgaccgcgt ttatcacgaa tggggaacat cacccgctcg
     4801 cggaaacgat cgtaactgcg cccctgatcg ttagtgacca acatgcccgc atcaatcaat
     4861 gactggcgat tttctggatt gccgccaaac cgcttcagga cgttgtccca gccggggggc
     4921 gcaaaaccaa tcgcaaagcg agcgataacc tcgtggctta atccgcgttt ttccagatac
     4981 tggcgcgcag acatggcaac aggttgttgt aaagattgtt ggtaaaacgt attcagaccg
     5041 tccatcaact gataaagcgt ttgcctctga tggcgctcga tctggctggg gccgctgcct
     5101 gcttcaaatg gcacttcaag attgtgcatt gctgccagct cttcgaccgt ttcgacgaac
     5161 tcgagcttgt cgtagttcat caggaagtcg atcgcgttgc cgtgcgcgcc acatccaaag
     5221 cagtggtaaa actgtttctc accgttaacg gtgaatgacg gggttttctc gttgtggaat
     5281 ggacaacacg cgtggaaatt cttgccctgc tttttcagct tcacacgggc atcgatcaga
     5341 tcgacgatgt cagtgcgtgc cagcagatca ttaatgaata cgcgtgggat tcgtccagcc
     5401 ataggccccg atttttagca attcataaac gaaaataagc cgcgcattcc tttcggaagc
     5461 acggccttac aactacaact cggtctgatc ggagagcaac gctctcgggg aattagtaca
     5521 gacgagtgcg gcgtgcgttt tcgcgagcca gtttcttcgc gtgacgtttc actgcagaag
     5581 ctttagcgcg cttacgttcg gtagtcggtt tttcatagaa ctcacgacga cgaacttccg
     5641 ccagaacacc tgctttttcg caggaacgct tgaagcgacg cagagctacg tcgaacggct
     5701 cgttttcacg tactttaatt accggcatgt gcctctcacc tttgattaat tcggtttgcc
     5761 gctggcatca acgccagctt atttcaaaat ggtgcggaat tttactgcaa ttgctgctgc
     5821 tttgtaaagc accgcggcct tttttgaaag ggacttttat aagggtgagg agtatacacg
     5881 aggctttctc caggggcgaa caaagtttta catcaacccg cattggtcct acactgcgcg
     5941 gtaataaagc gaggtaaaac aagtcatgcg tgtactgggt attgaaactt cctgcgatga
     6001 aaccggcatc gccatttacg acgatgaaaa aggtttgtta gccaaccaat tgtatagtca
     6061 ggtgaaattg cacgctgact acggcggcgt cgtgcctgaa ctggcctccc gcgatcacgt
     6121 gcgtaaaacc gtaccgttga tccaggaggc gctaaaggag tctggtttaa cggcaaaaga
     6181 cattgatgct gtggcctata ccgcaggccc aggattagtc ggcgcactgc tggttggcgc
     6241 gaccgtgggg cgttctctgg cgtttgcctg ggacgttccg gcaatcccgg tacatcatat
     6301 ggaaggacat ctgttagcgc cgatgctgga agataaccca ccggaatttc cgtttgtcgc
     6361 gctgctggtt tccggcggtc atacgcagtt aatcagcgtg actggcattg gtcagtacga
     6421 gctgctcggc gagtctatcg atgatgctgc tggtgaagcg tttgataaaa ccgcgaagct
     6481 gctggggctg gattatcctg gcggaccgtt actgtctaaa atggcggctc agggtactgc
     6541 cgggcgcttt gttttcccgc gtccgatgac cgaccgtccg gggctggatt tcagcttttc
     6601 tggtctgaaa accttcgcgg caaataccat acgtgacaac ggcactgacg atcagacgcg
     6661 tgccgatatc gcccgcgcct ttgaagatgc agtggtcgat acgttgatga ttaagtgtaa
     6721 gcgagcactg gatcagacgg gctttaagcg actggtcatg gcaggcggcg tgagtgctaa
     6781 ccgcacgttg cgggcgaaac tggcggaaat gatgaaaaaa cgccgtggcg aagtgttcta
     6841 cgcgcgtccg gaattttgta cggataacgg tgcgatgatc gcctatgccg gaatggtgcg
     6901 gtttaaagca ggcgcgacgg cggatctcgg cgtgagtgtg cgtccgcgct ggccactggc
     6961 agagttaccg gccgcgtaaa actattgggt gccggagagc aatttccggc accgtcctca
     7021 cttaaagcaa caccacgggc atccacagta agccggtaat caccagcaat acgaggaata
     7081 tcagcccaaa aatcgccccc agtcgccagt aatccgccgt tggcagataa ccactgccgt
     7141 agtaaatcgg actgggtccg gtggcgtatg gtgtaagaat gctccccaga ccaattgccg
     7201 caccaaccat caggcagaac accggcagcg ggatttccgg catcgccagc gcggcggcaa
     7261 tcatcatcgg tgcgagagcg gaggtatacg ctgtggcgct ggcaaaaaag tagcgcagca
     7321 gataaaagac cacaatcaac gccaccatca ctatcgttgg cgaataaccg cttaagctgc
     7381 ctgctaacag tttgccaaac cagctaataa aaccggtgtt gttgagtccg gtagcgaggg
     7441 tgataagcga ggccagccag aagaaaacgt tccacgccgc tttattactg acaatgtcgt
     7501 cccaactgat aatgcgcaga agcagcatca gcgccaccac gctgtaaccg accatcgcgg
     7561 catcgatata atcaccgcca aaaatccaca acaccagtgc gcctaccatc agccccagca
     7621 tccgtttttc acgcgaacag agcgggccca ttgcctgcag ttccgtctct gcccagcgcg
     7681 gcacctgatc gccagacttc agtaccggcg ggtacagcac gtaagccagc cagggaacca
     7741 gcaaaaccag taaaatgctg agcggcaaca tcccgaggaa ccagtcgccc cagcttagca
     7801 tggcgtgaga tgcgcttttc atcagtccaa ttaacagcaa atttggagcc atcgccgtca
     7861 ggaaaatggc gctggtcacg cagtcggcaa caatccccat ccacatgatg tacgagccaa
     7921 tgctgcgcga actgctgtcg tttggttgtg attgatagag cggtggcagg ttacggatga
     7981 tgggatagat aatccccgca ccacgcgccg agttggacgg tgttacaggc gcgagtatca
     8041 gctcggagaa catcactgca tagccgagaa acagcgtgcg atgccccatt tttttcacca
     8101 gaatcagcgc gatgcgacgt ccaagcccgg ttttttcata gcccgtacca aacataaagg
     8161 cggcgaaaat aagccagata actgaattag aaaaaccaga aaccgcccag gagagggatt
     8221 tggcggtgaa cttaaaacct ggctgggcga gctgctccgg gctaaacagc agccacggcg
     8281 aaagaatggc gatgatggat atacccacca tcgccaccac ggcacccggc acgggttcga
     8341 ggatcagtcc aacgatcacg ccagtaaaaa cggcaaagta gagccaggta tgactctcca
     8401 gccctgcggg aagcggaagt agagcaataa tggcgatgac cgccagcggc gcaaggtatc
     8461 gccaccattc agtggaaggt ttcatcgtgt tatgcctgcg aaaaaggaga ggggcgagcc
     8521 tctccgtgag ggttatttga tgtagtggac gtgctcgcag atctcttcca cgatgggatc
     8581 gcggcgttcg gcgaacagct ttttattttc ggctatcagg ttgttgccgt gggtatctat
     8641 agagacaatc agcgggccaa actctttgac ccggcagacc cacagcgact ctggcatccc
     8701 gagttctgtc cagtgcactt cttcaatctc ttccacttga gttgccgcca gtaccgcaca
     8761 gccagccggg aaaatcacat gcagcgcctt gaatttctgg cagccttctt cggtcagcgg
     8821 ccccattccg cctttgccga ccaccagttt cacgccggtc tgctcgataa attcacgttc
     8881 aaaactttcc atacgcatac tggttgtcgg gccgacggat accatctccc atttgtcgcc
     8941 gtttttgcgc acgatggggc cagcgtggaa aatcgctttg ccgttgagat cgtaagggat
     9001 cggacgtttc agttcgatca agcgacggtg acaaacgtca cggcaggtca ccagcgtacc
     9061 ggtcaggtag atcacatcgc cgacgcgaat atcttgcaga tcttcagctt tgatcggggt
     9121 tgtcaggatc tttttcataa cgcgctccgg gtgtgagaca gattttcaaa ggtgagatcg
     9181 gcatgaacca gcagtgtgcc gcgacgatgc gcccagcagc cggtagaaac agcaacgccg
     9241 atggttgacg gatggcgagc ggcagattcg atatgcacgc ccatcactga actgttgcca
     9301 gtcagccctt gtggaccaat ccccaaacgg ttgagtcctt cttccaggcg tagctccagc
     9361 tctgccgctt tcggattggg gtggcggctg ccaatcgggc gcaaaatggc ttttcgcgag
     9421 agcactgcgg cggtttccac cgaggtagcg atgccaacgc ccaccagtac cggcggacag
     9481 gcgtttacgg cgagggtgga gatattttcg aagacgaatt tcactacgcc ttcgtagcct
     9541 tctgacggca ttaacacttt cgatcggcca ggtagcgtgc agccgccgcc tgccatgtaa
     9601 acttcgattt ccgcatcgtc accgtcgggg acgatatccc aggtgaccca cggtacgccg
     9661 ctgccggtat ttttgccggt gtttacttcg tcaaaaattt ctaccgcatt gtgacgcagt
     9721 ggcgctttga cggttgcctc ttccacggct tgtttgagta tgctttgcag ctcgccaagc
     9781 agtgggaaac gggaaccgac cttaacaaaa aacatgattt cgccggtgtc ctgacaggca
     9841 ggacgattca ggtcgatcgc tttttgcatg ttatcgaaca tcgtgtggta gatgattttt
     9901 cccatcgacg atgtttcggc atcctttagc tgttttagtt tatccaccac gtcatcaggc
     9961 attcgggtag aaatcatggc ggtaaagtta gcgacaatct ccgtcaactt attcactgcc
    10021 tgttgcttat tactttcgct catcatggca actccagttt atgtatttgt ctggaaaaat
    10081 tcaccgccac aaactaccac taaaaatgtg gttttatatt ttttgccacc gtgaatatgt
    10141 aattaacagt tcgtgaatca acgcgaaaat aagaacgaat attgcgccag agcagggaat
    10201 ggtaatgttt tgcgaaatac actgcgttta tcaaaactgc ggatatttaa caatgctaaa
    10261 tagctggcct ttagccaaag acttgcaggt actggtggaa atcgtccata gcgggagttt
    10321 tagtgctgca gcggcgacgc ttgggcaaac gcctgcattt gtcaccaagc ggatccaaat
    10381 ccttgaaaat acgctggcaa caacgctgct gaaccgctcc gctcgcggcg tggcgctgac
    10441 tgaaagtggc caacgttgtt acgaacatgc gctggaaatc ctcacccaat atcagcgact
    10501 ggtcgatgac gtcacgcaaa tcaaaacgcg cccggagggg atgatccgca ttggttgcag
    10561 ttttggtttt gggcgcagcc atattgcgcc agctattacc gaactgatgc gcaattatcc
    10621 tgagttacag gtgcattttg aattgttcga tcgacaaatt gatttagtgc aggataatat
    10681 tgatctggat attcgtatta atgacgcaat tcctgattat tatattgcgc atcttttgac
    10741 aaaaaataaa agaatattat gcgcagcacc tgagtatctg caaaaatatc cgcagccaca
    10801 atccttacag gaattaagtc gtcatgactg cctggtgacc aaagagcgcg atatgaccca
    10861 cggaatatgg gagttgggga acggtcagga gaaaaaatcg gtgaaggtaa gcggacatct
    10921 ttcctccaat agcggcgaga ttgtgttgca atgggcgctg gagggcaaag ggattatgct
    10981 gcgttccgag tgggatgtgt tgccgtttct ggagagtggc aaactggtgc gagtattgcc
    11041 ggagtatgca caaagcgcca atatctgggc tgtttaccgg gagccgctct atcgcagcat
    11101 gaaattacgt gtctgtgtgg aatttctggc ggcatggtgc cagcaacggc tgggcaagcc
    11161 cgacgaaggc tatcaggtca tgtagatcca ccagaaatca ctcgggatcc ttttcgcgct
    11221 ttcttttgaa tttcgtccag atttttgtct cctggcgacg ccacagacgc tggatgttgt
    11281 catgatgacg cagcaggatc aggcaagaga gcatcgaaac cgggaaggtg aattgtggct
    11341 taaaccacca gacataaaac ggagcaatca gtgcactgac aatcgctccc agcgacgagt
    11401 aaccactcaa tagcacggtc agtaaccagg ttcccgccat tactccagtg agatcccagc
    11461 caattggtgc gatggcacca aaagcggtag caacgccttt tcctccttta aaaccgaaga
    11521 aaacgggcca gatgtgtcca agacaggcgg cgatggcaat taagcctagc cagaaggggc
    11581 tgacacctaa ttcatacgcg ccccagacgg gcaacattcc tttcagaacg tcgaaaatca
    11641 gtactgctac ggctgctccc ttgccaccga tacgtaacac attggttgcg cctggattgc
    11701 cggagccgct ggttcgcgga tcgggtaacc cacacaagcg gcaaaccaga atggcactgg
    11761 aaatggagcc gcagaggtac gcgatgagga tcattccagg cgcgattgca ctcataagct
    11821 gttccgtttt gaaaattcgt gttcaacgat gaatctgtgg ataatacgca catttcgccg
    11881 gaagtggtat ccggttagcc aaaaagcagg caggacgtga tggatattgt atttatagag
    11941 caactttcgg taatcaccac tattggtgtt tacgactggg aacagaccat cgaacagaag
    12001 ttagtgttcg atatcgaaat ggcgtgggat aaccgtaaag cggcgaaaag tgatgatgtg
    12061 gcggattgcc tcagttacgc tgacattgca gaaacggtgg tcagccacgt cgagggggcg
    12121 cgttttgcgc tggtggaacg cgtggctgaa gaggtggcgg aactgctgtt agcacgcttc
    12181 aactcgccgt gggtgcgtat caaactcagc aagccaggcg cagtggcgcg ggcggcgaat
    12241 gttggcgtaa tcattgagcg tggcaataat ctgaaagaaa ataattaatt ttacagctgt
    12301 taaaccaaac gattataacc tggtcatact gcagtagttc ggacaagcgt tacattttta
    12361 taatttaggg gtttattgat gagcgatatg cactcgctgc tgatagcggc aatattgggt
    12421 gtggtcgaag gattgacaga atttctgccg gtatccagca cgggccatat gattattgtc
    12481 ggtcacttgt tggggtttga gggcgacacg gcgaaaacct ttgaagtcgt gatccagtta
    12541 ggatcaattc tggcggtagt agtgatgttc tggcggcgtc tgtttggcct gattggcatc
    12601 cactttggcc gcccgttgca gcacgaagga gaaaacaaag gtcgtttaac gctgatccac
    12661 attttgctgg ggatgattcc ggcggtggta ctggggctgt tgttccacga cacgattaaa
    12721 tcattgttta acccgataaa tgtcatgtat gcactggtcg ttggcggtct gttgctgatt
    12781 gctgccgaat gcctgaagcc gaaagagccg cgtgcgccgg gtcttgatga tatgacctat
    12841 cgtcaggcgt ttatgattgg ctgtttccag tgtctggcgc tgtggccggg tttctcccgt
    12901 tccggggcga ccatttcagg tgggatgctg atgggggtga gccgttacgc tgcttccgag
    12961 ttctcgttcc tgctggcggt gccgatgatg atgggcgcaa cggcgctcga tctctacaaa
    13021 agctggggct tcctgactac cggcgatatc ccgatgtttg ccgttgggtt tatcaccgct
    13081 tttgtggtgg cgctgatagc gattaaaacc ttcctgcaat tgattaagcg catttcgttt
    13141 atcccgttcg ccatttatcg ctttattgtg gcggctgcgg tgtacgtcgt gttcttttga
    13201 ttgagagata gtgtcggata tgacgcttgt gcgtcttatc cgacctaagg gatttctgca
    13261 atatactgaa tttaaaaagt taatcaggct ttgggcaacg ttgttccttc cagctggcta
    13321 ccgccgcaat ccgccgtcgg gtcagctcct cacgaatctc cacaccttta aatcctgctt
    13381 caacgacggc ttttgtcggc actgactgcg ccacttccca ggcttcgcgc aaccagcggc
    13441 cttgcgggta gtccgctgat tcaaaaccgg ttctgccgcg cacgtcagcc tcgctggtca
    13501 gcgccagttg ctcgacacgc tgcggtttac gccaggcgtc aatggaatca aacaatttaa
    13561 caatggtttt cgggttcagc attggaaagg tgtggatgag atcgtgaaac tcagctacca
    13621 gtctggctaa atcgcgaatt tcatttggca cgcgcagacg ctggcatagt tgttccacta
    13681 acttcacacc cgccgggcca tgaccatgat gacgtggcca aagttctggc ggcgtcagtc
    13741 ctttaccgag atcgtggcat aacgttgcga aacgaacatc gacctgcgga ctcagcatcg
    13801 ccgccattga gagcgtcatt aaggtatgaa tacccgtgtc gatttccggg tgccacctgg
    13861 caggggccgg aacgccaaac agggcgtcaa tttccgggaa taaaacgcgc agtgcgccgc
    13921 aatcgcgcag tacctggaag aacacctggg gattgcgggt ggtgagggcg ctttccgtct
    13981 ctttccatac ccgttcaggc gtcaggtgtt ccagttcacc cgcatgggtc atctcgcgca
    14041 tcagcgccag agtttcatcg gcaatacgga aactgaggtg ggcataacgc gcagcaaaac
    14101 gcgctacgcg caacacgcgt aacggatctt cgccaaaagc gggggaaaca tggcgcaaca
    14161 gacgattttg cagatcgccc agaccgttgt acgggtcgat aatctcgccg ttatcgtcct
    14221 gggccagcgc attaatggtc agatcgcgac gcttaagatc atcttccagc gagacatccg
    14281 gtgcggcata gcaggtaaaa ccggtgtaac cggaaccaga tttccgttcg gtacgtgcca
    14341 gtgcatactc ttcatgcgtt tgcggatgca gaaacacagg aaaatcgcgg cctacctgct
    14401 ggtagcccgc gtcgagcatc tcctgtggcg tactgccgac caccacccaa tctctgtctt
    14461 tgaccggtag ccctaacaat gcatcccgaa cagcaccacc gaccagataa atcttcacgc
    14521 catcatccct tcgtcaaata cacaattcct aataataaga cagtgcaacg aagaaggcga
    14581 tttagttcat ccagcgatct ttgcgtttgc ggcttgggat caggtgcggc agtaccagac
    14641 caagcagcaa gccaagcccc agcacgccac cgccatacat aaaccactgc atgatgatgg
    14701 tgcgctgttt gtcatccaac tgtacgctgg cggcatcgac ctttttctgc gcgacaatca
    14761 gctcgttttt cagtttctga ttttcttctt ttaacctgtt gatcacgctg tcactctgcg
    14821 ccactttttg ctgcatttct gcggtgcgct gattccaggt gttatcgata ttggtgagtt
    14881 tatcggtcag agttttgacc tgattttcca gatctggcac acgggagcgc aggcttggtt
    14941 cagtgctaag ttgtttcaac gggatccagg cagtacggcc agagctgtct ttcacctggg
    15001 cataattggt gttggcgtca gtttgtaata aggtcacttc ctcgccggcg ttaaccgtgc
    15061 ccacgaggcg ataatgatct cccggaccgc tacggaccca ggtattcagt tcgtcggaaa
    15121 catagcgcgt ttcttcagcg tgtgagacgg cagtcgcgct aagtgcgagt aaagttaatc
    15181 cgatcaggcg taattttggc atcaggctgt cgttattgtc atggaaagtg gaacgatagt
    15241 agtggcatca gtgtcgctac gcaaagcatt cgacatcaat cggaatcctc tgtgtcaggt
    15301 cttgccactg cttcaaactt ttacgcccgt caaattgccc gttgcatggc aatttggcgc
    15361 aaaatactat ctactgacaa aaaagatcgc attaagttcg ccgttcatcg tcacttatgt
    15421 gacataacca gtaagtaaag gccatggctc aggaaatcga attaaagttt attgttaatc
    15481 acagtgccgt tgaggcgctg cgtgaccatc tcaatacgct gggcggcgag caccatgacc
    15541 ccgtgcagtt gctgaatatt tactacgaaa cgccggataa ttggctgcgt gggcacgaca
    15601 tgggcttgcg tattcgtggc gaaaacggtc gctatgagat gaccatgaaa gttgcaggaa
    15661 gagtgacagg cggcttacac cagcgcccgg aatataacgt ggcgttgagc gaaccgacgc
    15721 tcgacctggc gcagttaccg acggaagtct ggccgaacgg cgaattgcct accgatctcg
    15781 cttcccgcgt gcagccgctg tttagcaccg atttttatcg cgaaaaatgg ctagtagagg
    15841 tcgatggcag ccgaattgaa atcgctctcg atcagggtga ggtgaaagcg ggtgaatttg
    15901 ctgaacctat ctgtgagctg gaactggaac tgcttagcgg cgacatgcgt gcggtgctga
    15961 aactggcgaa ccaactggta tcgcaaaccg gattacgcca gggcagcctg agcaaagcag
    16021 cgcgtggcta tcatctggcg cagggcaatc cggcgcgtga aatcaaaccg accaccattt
    16081 tgcatgttgc ggcaaaagct gatgtggaac aggggctgga agcggcgctc gagctggcgt
    16141 tagcgcaatg gcagtatcat gaagaactgt gggtacgcgg caacgatgcg gcgaaagagc
    16201 aggtgctggc agccattggc ctggttcgtc atacactgat gctgttcggt ggcattgtgc
    16261 cgcgtaaagc gagcactcac ttacgtgatc tgctgactca atgcgaggcg accattgctt
    16321 ctgcggtttc tgccgtaacg gcggtctact ctaccgaaac ggcaatggcg aagctggcgt
    16381 tgaccgaatg gctggtaagc aaagcctggc agccgttttt agatgccaaa gcgcagagta
    16441 aaatgagcga ttccttcaaa cgctttgccg atatccatct ttcccgccat gccgctgaac
    16501 tgaaaagcgt tttctgccag ccgttaggcg atcgctaccg tgaccaattg ccacgcctga
    16561 cgcgtgatat tgactcaata ctgttgctgg cgggttacta cgatcctgtc gtcgcgcaag
    16621 actggctgga gaactggcag gggctgcgtc acgctattgc gaccgggcaa cgcattgaaa
    16681 ttgaacattt ccgtaatgag gcaaacaatc aggaaccgtt ctggttgcac agcggaaaac
    16741 gttaatcagg caaggataaa atcgcttatg aagccgctct cttcaccgtt acagcagtac
    16801 tggcagaccg ttgttgagcg gctgccagag cctttagccg aggaatcact tagcgcacag
    16861 gcgaagtcag tacttacttt cagtgatttt gtgcaggaca gcgtgattgc gcatccagag
    16921 tggctgacgg aactggaaag ccaaccgccg caggccgacg aatggcagca ttacgctgca
    16981 tggttgcagg aggcgctcag taatgtgagt gacgaggctg ggttaatgcg cgagctgcgg
    17041 ctattccggc ggcgcattat ggtgcgcatc gcctgggcgc aaacgctggc actggttact
    17101 gaagagagca tattgcagca gctcagccat ctggcggaga cgctgattgt tgcggcgcgt
    17161 gactggctgt atgacgcctg ctgccgcgaa tggggaacgc cgtgcaatgc gcagggcgaa
    17221 gcgcaaccgc tgctgatttt aggcatgggt aagctgggcg gcggggaatt gaatttctcc
    17281 tcagatatcg atctgatttt tgcctggccg gaacatggtt gtacccaggg cggacgccgg
    17341 gaactggata acgcgcagtt ttttacccgc atggggcagc ggctgattaa agtactggat
    17401 caaccaacgc aggatggctt cgtctatcgc gtggatatgc ggctgcgtcc gtttggggaa
    17461 agtggcccgc tggtgctgag ctttgcggcg ctggaagatt attaccagga gcaggggcgc
    17521 gactgggagc gttacgcgat ggtcaaggcg cggattatgg gggatagcga cggcgtctat
    17581 gctaacgagt tgcgtgcgtt gctgcgcccg tttgttttcc gtcgttacat cgatttcagc
    17641 gtgattcagt cgctgcgcaa catgaaagga atgattgccc gtgaagtgcg tcgacgcggt
    17701 ttgaccgaca acatcaaact tggcgcaggc ggcattcgcg aaattgaatt tatcgtccag
    17761 gtgttccagc tcattcgcgg cgggcgcgaa ccgtcgctgc aatcgcgctc tttactgcca
    17821 acgctcagcg ccattgccgc gctgcatctg ctttctgaaa acgatgctga acaattgcga
    17881 gtggcgtatc tgttcctgcg gcgtctggaa aacctgctgc aaagcattaa cgacgaacaa
    17941 acccagacgc ttccttctga tgagcttaac cgtgcgcggc tggcgtgggc gatggacttt
    18001 gctgactggc cgcaattgac cggggtgctg accgcacata tggccaatgt gcgccgggtg
    18061 tttaatgaat tgattggcga cgacgaaagt gaaactcagg aagagtcgct gtcggaacag
    18121 tggcgtgagc tgtggcagga tgcgttgcag gaagatgaca ccacgccagt gctggcgcat
    18181 cttagcgagg atgatcgcaa acaggtgcta atgctgattg ctgatttccg caaagagctg
    18241 gataagcgca ccatcgggcc gcgaggacgt caggtgctcg accatctgat gccacatctg
    18301 ctaagtgatg tctgtgcgcg tgaagacgct gctgtcacgc tgtcgcgcat taccgccttg
    18361 ctggtgggga ttgttactcg caccacttat cttgagctgc ttagtgaatt ccctgcggcg
    18421 cttaagcatt tgatttctct gtgtgctgca tcgccgatga ttgccagcca gctagcgcgt
    18481 tatccattat tgctggatga attgctcgat ccaaataccc tctaccaacc aacggcgacg
    18541 gatgcctacc gtgatgaact gcgccagtat ttgctgcgcg tgccggaaga tgacgaagaa
    18601 caacagcttg aggcgctgcg tcagttcaaa caggcgcagc tgttacgcat tgcggcagcg
    18661 gatatcgccg gtacgctacc ggtaatgaaa gtgagcgatc acttaacctg gctggcagaa
    18721 gccatgattg atgccgtcgt tcagcaggcg tgggttcaaa tggttgcccg ctacggtaag
    18781 ccaaatcacc tgaacgaacg cgaagggcgt ggttttgcgg tggtcggcta cggcaagctg
    18841 ggtggctggg agttaggcta cagttccgat cttgacctga tcttcctcca tgattgccca
    18901 atggatgcga tgactgacgg cgagcgggaa atcgacgggc gacagtttta tctgcgtctg
    18961 gcgcaacgca ttatgcatct gttcagtacg cgaacctctt ccggcatttt gtatgaagtg
    19021 gatgcacgac tgcgtccgtc cggggcggcg ggaatgctgg tgacatccgc agaagcattt
    19081 gctgattatc agaaaaacga ggcctggacg tgggagcatc aggcgctggt gcgtgcgcgt
    19141 gtagtgtacg gcgatccgca gctcaccgcg cactttgacg cagtacgccg ggagattatg
    19201 acgctgccgc gcgaaggcaa aaccctgcaa acagaagtgc gggaaatgcg cgagaaaatg
    19261 cgtgcccatc tcggcaataa acatcgcgat cgctttgata tcaaagccga tgaaggcgga
    19321 attaccgata tcgaatttat tactcaatat ctggtgttgc gctacgctca cgaaaaaccg
    19381 aagttaacgc gctggtcaga caacgtgcgc attctggaac tactggcgca aaacgacatt
    19441 atggaagagc aggaagcgat ggcgctgacc cgtgcttaca cgacgctgcg cgatgaactt
    19501 catcatctgg cattacagga attgccgggc catgtgtcgg aggattgctt caccgcagag
    19561 cgtgaactgg tgcgggcaag ctggcagaag tggctggtgg aagaatgaag tatggtatta
    19621 tcgcgcgcaa attttgaatc tctcaggaga caggaatgaa agtaacgctg ccagagtttg
    19681 aacgtgcagg agtgatggtg gttggtgatg tgatgctgga tcgttactgg tacggtccca
    19741 ccagccgtat ctcgccggaa gcgccggtac ccgtggttaa agtgaatacc atcgaagaac
    19801 gtccgggcgg cgcggctaac gtggcgatga atatcgcttc tctcggtgct aatgcacgcc
    19861 tggtcgggtt gacgggcatt gacgatgcgg cgcgcgcgct cagtaaatct ctggccgacg
    19921 tcaacgttaa atgcgacttt gtttctgtac cgacgcatcc gactatcacc aaattacggg
    19981 tactttcccg taaccaacag ctgatccgtc tggatttcga agaaggtttc gaaggggttg
    20041 atccgcagcc gctgcacgag cggattaatc aggcactgag ttcgattggc gcgctggtgc
    20101 tttctgacta cgccaaaggt gcgctggcaa gcgtacagca gatgatccaa ctggcgcgta
    20161 aagcgggtgt tccggtgctg attgatccaa aaggtaccga ttttgagcgc taccgcggcg
    20221 ctacgctgtt aacgccgaat ctctcggaat ttgaagctgt tgtcggtaaa tgtaagaccg
    20281 aagaagagat tgttgagcgc ggcatgaaac tgattgccga ttacgaactc tcggctctgt
    20341 tagtgacccg ttccgaacag ggtatgtcgc tgctgcaacc gggtaaagcg ccgctgcata
    20401 tgccaaccca ggcgcaggaa gtgtatgacg ttaccggtgc gggcgacacg gtgattggcg
    20461 tcctggcggc aacgctggca gcgggtaatt cgctggaaga agcctgcttc tttgccaatg
    20521 cggcggctgg tgtggtggtc ggcaaactgg ggacatccac ggtttcgccg atcgagctgg
    20581 aaaacgcagt acgtggacgt gcagataccg gctttggtgt gatgaccgaa gaggaactga
    20641 agcttgctgt agcggcagcg cgtaaacgcg gtgaaaaagt ggtgatgacc aacggtgtct
    20701 ttgacatcct gcacgccggg cacgtctctt atctggcaaa tgcccgtaaa ctgggtgacc
    20761 gtttgattgt cgctgtgaac agcgatgcct ccaccaaacg gctgaaaggg gattcccgcc
    20821 ccgttaaccc gctcgaacag cgtatgattg tgctgggcgc actggaagcg gtcgactggg
    20881 tggtgtcgtt tgaagaggac acaccgcagc gcttgatcgc cgggatcttg ccagatctgc
    20941 tggtgaaagg cggcgactat aaaccagaag agattgccgg gagtaaagaa gtctgggcca
    21001 acggtggcga agtgctggtg ctcaactttg aagacggttg ctcgacgacc aacatcatca
    21061 agaagatcca actggataaa aaaggctaac tggaaagcgg ttcacagatc tcgcttcgta
    21121 gcaggcaggt ctattgacgc tgttgttttt tcaacctaaa gtaaaggaac aagggtaagg
    21181 gaggatttct cccccctctg atgagttgtt agtaagtcgg gaaacttaac agtaacaaca
    21241 caaccagtat gatgacgaac ttcatcataa ccctttcctt ctataaggcc ccttcctcgg
    21301 gaggggcttt cccgtttcag ccccttgctg actccccgat tgttgatgag cgccggaccc
    21361 cgcgcaacgc tttcccagag gcagttttgc cttccccgag cagtcacgca aaatgctgca
    21421 acagcctgat catttgcacc ttttagcgga acgcagatat aaaaaatccc cggctttttg
    21481 ggccagggat tattcataga agagggttta ctctgctttt tcttcgacgg gagttgttga
    21541 gcttaaggat gaagtcaatg ccgccagtga gccgcctgaa acgccaattt cattgagcaa
    21601 ggagtcaatc agcggtgcct gtgtgcggta agagagtgcg gctgataacg cttgttctgc
    21661 caggtttccg ccccccacat tacccgtgtt tgcatcaccc gtagcgccgc cacgattcag
    21721 accatcgacc tgaataattt tgatgccgtc gatagacttc ataggctcaa cggatttctc
    21781 aattaccgca ggcagcgcct gcaataatgc cagtttgaat ttaaggctgg tttgttcatc
    21841 agaaagtacg ttgatagcat cgttcagcgc acgttgtgct tctgcttccg ccagaccctt
    21901 tttacgtgta gcttcggcta actcaacgat agccgccgcc tgcatttctg cggcttcttt
    21961 ttctgctttc gcccgcacgg tcagttcaac cgctttggtt tctgcatcct gcgctgcggc
    22021 gatcaacgcc acttgtttag cacgatcggc ttcggcagtc tggcgagtgg tttctacgtt
    22081 ttgttgggcg cttactgctt ctgcgagtgc aaggttagca cgcgcttctg cctgggactg
    22141 ttgttccgat ttggcggcaa tagcgatcga tttcgtctgg ttagcgattt cggtgacctg
    22201 ctgctgttcg atctctttaa tgcgaacttc acgttcggcc tcaacctttc ttgagcggac
    22261 ggcctgttcg cggtcgattt ccgtttcctg aatctgtcgt tcagccagaa ttcgcgtctg
    22321 ctctgcttca cgacgacgtt cagcttcaaa agccgcaatt ttcgcattct gctcagcggt
    22381 acgggtttta acctgctgct cctgctcaag cgtcataaac gcttcttgtt gttcaatctc
    22441 caacttgcgc gaaagtgcat cacggttttt ctcacgcact gcgacttcca catcctgttc
    22501 aacttcgtta cgttcgcgac gacggcgctc cgtctcctga gtcagtttgg ttaaaccttc
    22561 ggcgtcaaag gcattgttcg gattgaaatg ttctttcgag gtctggttaa agttggtaag
    22621 tgaaacgctt tccagttcca ggccattttt cgacagatct tcagctacgg tattttgtac
    22681 tccctgcacg aagttctcgc gggtatcctg taactcatgc atggtcattt gtgccgctgt
    22741 tgcacggagg gcatcgacaa atttatcttc caccagcata cgtaagtctt caggcgacag
    22801 ggtgcgttgc cccagcgtct gggcggcggt ggcaatccct tctactgaag gttttacccg
    22861 cacaaagaaa gcaaccacga catcgacgcg catacgatct ttcgtaatca ggctatcaat
    22921 ggttgagcgg ctgacttcca gcttcagggt attcatattg atgggaatta tttcatgaaa
    22981 gatcggcatc acgattgcgc caccgctcat caccactttt tgcccactta aaccagtacg
    23041 aacaaatgct tgctctgccg aagcgcgacg atagagcctg gcgaaaataa ttccaataat
    23101 aaacagaatg catacggcga taatcgcggt aaacatccag gagggcacag aattaacgat
    23161 atcatccatt attaaatttc cttttgatta ataaatcaca atatctgggg ccagggatta
    23221 ttttccgcca aatatcgcgt tgcagaaagt cggcaaataa ttaacacttt gtctccttta
    23281 gtgaaagttt ttccttcttc aggctccagt agcaaataat gtatttggcc aaattgatcg
    23341 gtgagttttc cttcgcaggg gttacctgaa gtggcttgat ggccggtaat taatgccatg
    23401 ctaccaacat attcttcttc tgtaatagct gaactatggt cgcgtggaat ccagggtgca
    23461 acgactttac cggtataatg cactgcaata attgtaaaca gtaaactgac aggaaccacg
    23521 aacaggttgg ggagtggtga ttgccagagc gtaacgcagg catgttgcaa caggatgcca
    23581 ataagaccaa aaaaaccagc gagtaagcag aggacgacga gagctggcaa tcttccaata
    23641 ttgagataat gaagtgcctg gctaatatga cctgtggtta tggaatcata atggtcaaga
    23701 tgtgcatcaa gagcaccgga gagcatgtga ccgcagataa gtgcaaaaat ttctaaaagg
    23761 ccgatcaaga gtacaaagga aatagcgaaa agataagggg tgttatagtc ggcgaataaa
    23821 atcatatgtc actccaatgt ccatttagtg acgcaatatt ta
//
LOCUS       DAHHTG010000069        22640 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.069, whole genome shotgun sequence.
ACCESSION   DAHHTG010000069 DAHHTG010000000
VERSION     DAHHTG010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22640)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22640)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22640
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.069"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            102..1187
                     /gene="mltB"
                     /locus_tag="NBI74_002699"
     CDS             102..1187
                     /gene="mltB"
                     /locus_tag="NBI74_002699"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase B"
                     /protein_id="HCD1933009.1"
                     /translation="MFKRRYVTLLPLFVLLAACSSKPKPTETETTTGTPSGGFLLEPQ
                     HNVMQMGGDFANNPNAQQFIDKMVNKHGFDRQQLQEILSQAKRLDSVLRLMDNQAPTT
                     SVKPPSGPNGAWLRYRKKFITPDNVQNGVVFWNQYEDALNRAWQVYGVPPEIIVGIIG
                     VETRWGRVMGKTRILDALATLSFNYPRRAEYFSGELETFLLMARDEQDDPLNLKGSFA
                     GAMGYGQFMPSSYKQYAVDFSGDGHINLWDPVDAIGSVANYFKAHGWVKGEQVAVMAN
                     GQAPGLPNGFKTRYSISQLAAAGLTPQQSLGNHQQASLLRLDVGTGYQYWYGLPNFYT
                     ITRYNHSTHYAMAVWQLGQAVALARVQ"
     gene            complement(1226..1465)
                     /locus_tag="NBI74_002700"
     CDS             complement(1226..1465)
                     /locus_tag="NBI74_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016249109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1933010.1"
                     /translation="MAIKKTDNTPQNVLKQTTQMRMWVIIGGISALISVICKVLSSGY
                     LEPFDFGQAALGVVCLGYAISLSKKIKQLRHSNKQ"
     gene            1647..2144
                     /gene="pncC"
                     /locus_tag="NBI74_002701"
     CDS             1647..2144
                     /gene="pncC"
                     /locus_tag="NBI74_002701"
                     /EC_number="3.5.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide-nucleotide amidase"
                     /protein_id="HCD1933011.1"
                     /translation="MTESELMQLSEQVGLALKARGATVTTAESCTGGWVAKVITDIAG
                     SSAWFERGFVTYSNEAKAQMIGVREETLAQHGAVSEPVVVEMAIGALKAARADYAVSI
                     SGIAGPDGGSEEKPVGTVWFAFATARGEGITRRECFSGDRDAVRRQATAYALQTLWQQ
                     FLQNT"
     gene            2224..3285
                     /gene="recA"
                     /locus_tag="NBI74_002702"
     CDS             2224..3285
                     /gene="recA"
                     /locus_tag="NBI74_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HCD1933012.1"
                     /translation="MAIDENKQKALAAALGQIEKQFGKGSIMRLGEDRSMDVETISTG
                     SLSLDIALGAGGLPMGRIVEIYGPESSGKTTLTLQVIAAAQREGKTCAFIDAEHALDP
                     IYARKLGVDIDNLLCSQPDTGEQALEICDALARSGAVDVIVVDSVAALTPKAEIEGEI
                     GDSHMGLAARMMSQAMRKLAGNLKQSNTLLIFINQIRMKIGVMFGNPETTTGGNALKF
                     YASVRLDIRRIGAVKEGENVVGSETRVKVVKNKIAAPFKQAEFQILYGEGINFYGELV
                     DLGVKEKLIEKAGAWYSYKGEKIGQGKANATAWLKDNPETAKEIEKKVRELLLSNPNS
                     TPDFSVDDSEGVAETNEDF"
     gene            3354..3854
                     /gene="recX"
                     /locus_tag="NBI74_002703"
     CDS             3354..3854
                     /gene="recX"
                     /locus_tag="NBI74_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HCD1933013.1"
                     /translation="MTESTSRRPAYARLLDRAVRILAVRDHSEQELRRKLAAPIMGKN
                     GPEEIDATAEDYERVIAWCHEHGYLDDSRFVARFIASRSRKGYGPARICQELNQKGIS
                     REATEKAMRECDIDWCALARDQATRKYGEPLPTVFSEKVKIQRFLLYRGYLMEDIQDI
                     WRNFAD"
     gene            3983..6613
                     /gene="alaS"
                     /locus_tag="NBI74_002704"
     CDS             3983..6613
                     /gene="alaS"
                     /locus_tag="NBI74_002704"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HCD1933014.1"
                     /translation="MSKSTAEIRQAFLDFFHSKGHQVVASSSLVPHNDPTLLFTNAGM
                     NQFKDVFLGLDKRNYSRATTSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDY
                     FKHDAIQFAWELLTSEKWFALPKERLWVTVYESDDEAYEIWEKEVGIPRERIIRIGDN
                     KGAPYASDNFWQMGDTGPCGPCTEIFYDHGDHIWGGPPGSPEEDGDRYIEIWNIVFMQ
                     FNRQADGTMEPLPKPSVDTGMGLERIAAVLQHVNSNYDIDLFRTLIQAVAKVTGATDL
                     SNKSLRVIADHIRSCAFLIADGVMPSNENRGYVLRRIIRRAVRHGNMLGAKETFFYKL
                     VGPLIDVMGSAGEDLKRQQAQVEQVLKTEEEQFARTLERGLALLDEELAKLSGDTLDG
                     ETAFRLYDTYGFPVDLTADVCRERNIKVDEAGFDAAMEEQRRRAREASGFGADYNAMI
                     RVDSASEFKGYDHLELNGKVTALFVDGKSVDAINAGQEAVVVLDQTPFYAESGGQVGD
                     KGELKGANFSFAVEDTQKYGQAIGHIGKLAVGSLKVGDAVQADVDEARRARIRLNHSA
                     THLMHAALRQVLGTHVSQKGSLVNDKVLRFDFSHNEAMKPEEIRAVEDLVNAQIRRNL
                     PIETNIMDLEAAKAKGAMALFGEKYDERVRVLSMGDFSTELCGGTHASRTGDIGLFRI
                     ISESGTAAGVRRIEAVTGEGAIATVHADSDRLSEVAHLLKGDSNNLADKVRSVLERTR
                     QLEKELQQLKEQAAAQESANLSSKAIDVNGVKLLVSELSGVEPKMLRTMVDDLKNQLG
                     STIIVLATVAEGKVSLIAGVSKDVTDRVKAGELIGMVAQQVGGKGGGRPDMAQAGGTD
                     AAALPAALASVKGWVSAKLQ"
     gene            6848..7033
                     /gene="csrA"
                     /locus_tag="NBI74_002705"
     CDS             6848..7033
                     /gene="csrA"
                     /locus_tag="NBI74_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HCD1933015.1"
                     /translation="MLILTRRVGETLMIGDEVTVTVLGVKGNQVRIGVNAPKEVSVHR
                     EEIYQRIQAEKSQQSSY"
     gene            7349..7441
                     /locus_tag="NBI74_002706"
     tRNA            7349..7441
                     /locus_tag="NBI74_002706"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7383..7385,aa:Ser,seq:gct)
     gene            7445..7521
                     /locus_tag="NBI74_002707"
     tRNA            7445..7521
                     /locus_tag="NBI74_002707"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7479..7481,aa:Arg,seq:acg)
     gene            7585..7661
                     /locus_tag="NBI74_002708"
     tRNA            7585..7661
                     /locus_tag="NBI74_002708"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7619..7621,aa:Arg,seq:acg)
     gene            7942..8508
                     /gene="yqaB"
                     /locus_tag="NBI74_002709"
     CDS             7942..8508
                     /gene="yqaB"
                     /locus_tag="NBI74_002709"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-1-phosphate/6-phosphogluconate
                     phosphatase"
                     /protein_id="HCD1933016.1"
                     /translation="MYERYAGLIFDMDGTILDTEPTHRKAWREVLGHYGLQYDVQAMI
                     ALNGSPTWRIAQAIIELNQADLDPHALAREKTEAVRSMLLDSVEPLPLVEVVKSWHGR
                     RPMAVGTGSESAIAEALLAHLGLRRYFDAVVAADHVKHHKPAPDTFLLCAQRMGVQPT
                     QCVVFEDADFGIQAARAAGMDAVDVRLL"
     gene            8505..8933
                     /locus_tag="NBI74_002710"
     CDS             8505..8933
                     /locus_tag="NBI74_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCD1933017.1"
                     /translation="MSEALSLFSLFASSFLSATLLPGNSEVVLVAMLLSGVSHPWVLV
                     LTATMGNSLGGLTNVILGRFFPLRKTSRWQEKATGWLKRYGAVTLLLSWMPVVGDLLC
                     LLAGWMRISWGPVIFFLCLGKALRYVAVAAATVQGMMWWH"
     gene            9006..10562
                     /gene="gshA"
                     /locus_tag="NBI74_002711"
     CDS             9006..10562
                     /gene="gshA"
                     /locus_tag="NBI74_002711"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HCD1933018.1"
                     /translation="MIPDVSQALAWLEKHPQALKGIQRGLERETLRVNADGTLATTGH
                     PEALGSALTHKWITTDFAEALLEFITPVDGDIEHMLTFMRDLHRYTARNMGDERMWPL
                     SMPCYIAEGQDIELAQYGTSNTGRFKTLYREGLKNRYGALMQTISGVHYNFSLPMAFW
                     QAKCGDISGADAKEKISAGYFRVIRNYYRFGWVIPYLFGASPAICSSFLQGKPTSLPF
                     EKTECGMYYLPYATSLRLSDLGYTNKSQSNLGITFNDLYEYVAGLKQAIKTPSEEYAK
                     IGIEKDGKRLQINSNVLQIENELYAPIRPKRVTRSGESPSDALLRGGIEYIEVRSLDI
                     NPFSPIGVDEQQVRFLDLFMVWCALADAPEMSSSELACTRVNWNRVILEGRKPGLTLG
                     IGCETAQFPLPQVGKDLFRDLKRVAQTLDSINGGEAYQKVCDELVACFDNPDLTFSAR
                     ILRSMIDTGIGGTGKAFAEAYRNLLREEPLEILREEDFVAEREASERRQQEMEAADTE
                     PFAVWLEKHA"
     gene            10712..11227
                     /gene="luxS"
                     /locus_tag="NBI74_002712"
     CDS             10712..11227
                     /gene="luxS"
                     /locus_tag="NBI74_002712"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008458247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HCD1933019.1"
                     /translation="MPLLDSFTVDHTRMEAPAVRVAKTMNTPHGDAITVFDLRFCVPN
                     KEVMPERGIHTLEHLFAGFMRNHLNGNGVEIIDISPMGCRTGFYMSLIGTPDEQRVAD
                     AWKAAMEDVLKVQDQNQIPELNVYQCGTYQMHSLQEAQDIARNILERDVRINSNEELA
                     LPKEKLQELHI"
     gene            complement(11278..12390)
                     /locus_tag="NBI74_002713"
     CDS             complement(11278..12390)
                     /locus_tag="NBI74_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001625743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCD1933020.1"
                     /translation="MIWQLTDDKRWSALRQRFSWVEEMHHTPQDPEHHGEGDVGVHTE
                     MVLNALITLPEFQQLPAQQQEVLWAAALLHDVEKRSTTVQENGRIQSPGHARRGELTA
                     RQILWRDIPTPFVLREQIVALVRLHGLPLWLLERPEPERLLLTAAMRIDTRLLALLAR
                     ADLLGRQSPDQQSMLERIDLFELFCHEQQCWGKMRPFVSDSARWHYLTHEQSSPDFVP
                     WEAEPFEVILLCGLPGMGKDRYISEQCQGMDVISLDDMRRRINASPDDKTATGRIVQQ
                     AKEEARVFLRQKKPFIWNATNITRQLRSQLISLFTAYGARVKIVYLEVPWAQWNQQNA
                     RREYAVPEAVVMRMASRLEVPQPDEAHSVEYRMTDR"
     gene            complement(12387..13094)
                     /locus_tag="NBI74_002714"
     CDS             complement(12387..13094)
                     /locus_tag="NBI74_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001613653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase family protein"
                     /protein_id="HCD1933021.1"
                     /translation="MNTQRKYGRTWHYPFSPGTTSDDRINTDYWQDLQTITQLVHTEK
                     LDGENNCLNRYGVFARSHATPTQSAWTYKIRQRWQLLKNDLGNLELFGENLYAVHSIE
                     YRALEQDFYLFAVRCQDMWLSWEEVQFYAALFDFPCVPEISGPQPGNDEKSWQRDFLA
                     LTNARGTFDPWDTQTCQPCTLEGIVSRNHDAFSVADFSHNVFKYVRKNHVKTTVHWKR
                     HWQRARMAHEFVYGEQS"
     gene            complement(13352..14890)
                     /gene="emrB"
                     /locus_tag="NBI74_002715"
     CDS             complement(13352..14890)
                     /gene="emrB"
                     /locus_tag="NBI74_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrB"
                     /protein_id="HCD1933022.1"
                     /translation="MQQQKPLEGAQLVIMTIALSLATFMQVLDSTIANVAIPTIAGNL
                     GSSLSQGTWVITSFGVANAISIPLTGWLAKRVGEVKLFLWSTIAFAIASWACGVSSSL
                     NMLIFFRVIQGIVAGPLIPLSQSLLLNNYPPAKRSIALALWSMTVIVAPICGPILGGY
                     ISDNYHWGWIFFINVPIGVAVVLMTLQTLRGRETRTERRRIDAVGLALLVIGIGSLQI
                     MLDRGKELDWFSSQEIIILTVVAVVAICFLIVWELTDDNPIVDLSLFKSRNFTIGCLC
                     ISLAYMLYFGAIVLLPQLLQEVYGYTATWAGLASAPVGIIPVILSPIIGRFAHKLDMR
                     RLVTFSFIMYAVCFYWRAYTFEPGMDFGASAWPQFIQGFAVACFFMPLTTITLSGLPP
                     ERLAAASSLSNFTRTLAGSIGTSITTTMWTNRESLHHAQLTESVNPFNPNAQAMYSQL
                     EGLGMTQQQASGWIAQQITNQGLIISANEIFWMSAGIFLVLLGLVWFAKPPFGAGGGG
                     GGAH"
     gene            complement(14907..16079)
                     /gene="emrA"
                     /locus_tag="NBI74_002716"
     CDS             complement(14907..16079)
                     /gene="emrA"
                     /locus_tag="NBI74_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrA"
                     /protein_id="HCD1933023.1"
                     /translation="MSANAETQTPQQPVKKSGKRKRLLLLLTLLFIIIAVAIGIYWFL
                     VLRHFEETDDAYVAGNQIQIMSQVSGSVTKVWADNTDFVKEGDVLVTLDPTDARQAFE
                     KAKTALASSVRQTHQLMINSKQLQANIEVQKIALAKAQSDYNRRVPLGNANLIGREEL
                     QHARDAVTSAQAQLDVAIQQYNANQAMILGTKLEDQPAVQQAATEVRNAWLALERTRI
                     VSPMTGYVSRRAVQPGAQISPTTPLMAVVPATNMWVDANFKETQIANMRIGQPVTITT
                     DIYGDDVKYTGKVVGLDMGTGSAFSLLPAQNATGNWIKVVQRLPVRIELDQKQLEQYP
                     LRIGLSTLVSVNTTNRDGQVLANKVRSTPVAVSTAREISLAPVNKLIDDIVKANAG"
     gene            complement(16206..16736)
                     /gene="emrR"
                     /locus_tag="NBI74_002717"
     CDS             complement(16206..16736)
                     /gene="emrR"
                     /locus_tag="NBI74_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter EmrAB
                     transcriptional repressor EmrR"
                     /protein_id="HCD1933024.1"
                     /translation="MDSSFTPIEQMLKFRASRHEDFPYQEILLTRLCMHMQSKLLENR
                     NKMLKAQGINETLFMALITLESQENHSIQPSELSCALGSSRTNATRIADELEKRGWIE
                     RRESDNDRRCLHLQLTEKGHEFLREVLPPQHNCLHQLWSALSTTEKDQLEQITRKLLS
                     RLDQMEQGGVVLEAMS"
     gene            complement(16827..17162)
                     /gene="ygaH"
                     /locus_tag="NBI74_002718"
     CDS             complement(16827..17162)
                     /gene="ygaH"
                     /locus_tag="NBI74_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine transporter subunit YgaH"
                     /protein_id="HCD1933025.1"
                     /translation="MSYEVLLLGLLVGAANYCFRYLPLRLRVGNARPTKRGAVGILLD
                     TIGIASICALLVVSTAPEVMHDTRRFVPTLVGFAVLGASFYKTRSIIIPTLLSALAYG
                     LAWKVMAII"
     gene            complement(17152..17889)
                     /gene="ygaZ"
                     /locus_tag="NBI74_002719"
     CDS             complement(17152..17889)
                     /gene="ygaZ"
                     /locus_tag="NBI74_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine exporter subunit YgaZ"
                     /protein_id="HCD1933026.1"
                     /translation="MESPTPQPAPGSATFMEGCKDSLPIVISYIPVAFAFGLNATRLG
                     FSPLESVFFSCIIYAGASQFVITAMLAAGSSLWVAALTVMAMDVRHVLYGPSLRSRII
                     QRLQKSKTALWAFGLTDEVFAAATAKLVRNNRRWSENWMIGIAFSSWSSWVFGTVIGA
                     FSGSGLLQGYPAVEAALGFMLPALFMSFLLASFQRKQSLCVTAALVGALAGVTLFSIP
                     VAILAGIVCGCLTALIQAFWQGAPDEL"
     gene            complement(18013..19197)
                     /locus_tag="NBI74_002720"
     CDS             complement(18013..19197)
                     /locus_tag="NBI74_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCD1933027.1"
                     /translation="MTKPNHELSPALIVLMSIATGLAVASNYYAQPLLDTIARNFSLS
                     ASSAGFIVTAAQLGYAAGLLFLVPLGDMFERRRLIVSMTLLAAGGMLITASSQSLAMM
                     ILGTALTGLFSVVAQILVPLAATLASPDKRGKVVGTIMSGLLLGILLARTVAGLLANL
                     GGWRTVFWVASVLMALMALALWRGLPQMKSETHLNYPQLLGSVFSMFISDKILRTRAL
                     LGCLTFANFSILWTSMAFLLAAPPFNYSDGVIGLFGLAGAAGALGARPAGGFADKGKS
                     HHTTTFGLLLLLLSWLAIWFGHTSVLALIIGILVLDLTVQGVHITNQTVIYRIHPDAR
                     NRLTAGYMTSYFIGGAAGSLISASAWQHGSWAGVCLAGATIALVNLLVWWRGFHRQEA
                     AN"
     gene            complement(19389..20381)
                     /gene="proX"
                     /locus_tag="NBI74_002721"
     CDS             complement(19389..20381)
                     /gene="proX"
                     /locus_tag="NBI74_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     substrate-binding protein ProX"
                     /protein_id="HCD1933028.1"
                     /translation="MRHSVLFATAFATLISTQTFAADLPGKGITVNPVQSTITEETFQ
                     TLLVSRALEKLGYTVNKPSEVDYNVGYTSLASGDATFTAVNWTPLHDNMYEAAGGDKK
                     FYREGVFVNGAAQGYLIDKKTADQYKITNIAQLKDPKIAKLFDTNGDGKADLTGCNPG
                     WGCEGAINHQLAAYGLTNTVTHNQGNYAAMMADTISRYKEGKPVFYYTWTPYWVSNEL
                     KPGKDVVWLQVPFSALPGDKNANTKLPNGANYGFPVSTMHIVANKAWAEKNPAAAKLF
                     AIMQLPVADINAQNAIMHDGKASEDDIQGHVDGWIKAHQQQFDGWVNEALAAQK"
     gene            complement(20438..21502)
                     /gene="proW"
                     /locus_tag="NBI74_002722"
     CDS             complement(20438..21502)
                     /gene="proW"
                     /locus_tag="NBI74_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     permease ProW"
                     /protein_id="HCD1933029.1"
                     /translation="MADQNNPWDTTPAADSAAQSADAWGTPAAAPTDGGGADWLTSTP
                     APNVEHFNILDPFHKTLIPLDSWVTEGIDWVVTHFRPVFQGVRVPVDYILNGFQQLLL
                     GMPAPVAIIVFALIAWQISGVGMGVATLVSLIAIGAIGAWSQAMVTLALVLTALLFCI
                     VIGLPLGIWLARSPRAAKIIRPLLDAMQTTPAFVYLVPIVMLFGIGNVPGVVVTIIFA
                     LPPIIRLTILGINQVPADLIEASRSFGASPSQMLFKVQLPLAMPTIMAGVNQTLMLAL
                     SMVVIASMIAVGGLGQMVLRGIGRLDMGLATVGGVGIVILAIILDRLTQAVGRDSRSR
                     GNRRWYTTGPVGLLTRPFIK"
     gene            complement(21495..>22640)
                     /gene="proV"
                     /locus_tag="NBI74_002723"
     CDS             complement(21495..>22640)
                     /gene="proV"
                     /locus_tag="NBI74_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein ProV"
                     /protein_id="HCD1933030.1"
                     /translation="RAFKYIEQGLSKEQILEKTGLSLGVKDASLAIEEGEIFVIMGLS
                     GSGKSTMVRLLNRLIEPTRGQVLIDGVDIAKISDVELREVRRKKIAMVFQSFALMPHM
                     TVLDNTAFGMELAGINAEERREKALDALRQVGLENYAHSYPDELSGGMRQRVGLARAL
                     AINPDILLMDEAFSALDPLIRTEMQDELVKLQAKHQRTIVFISHDLDEAMRIGDRIAI
                     MQNGEVVQVGTPDEILNNPANDYVRTFFRGVDISQVFSAKDIARRTPNGLIRKTPGFG
                     PRSALKLLQDEDREYGYVIERGNKFVGAVSIDSLKAALTQQQGLDAALIDAPLAVDAQ
                     TPLSELLSHVGQAPCAVPVVDEDQQYVGIISKGMLLRALDREGVNNG"
BASE COUNT         5543 a         5693 c         6124 g         5280 t
ORIGIN      
        1 gttatgtaaa gtccgtatcg ggcagtgact accgcttcct tgtgcggggc gggatgcttt
       61 accatacttg cccctggttg aatctgttaa atggacccct catgttcaag cgtcgttatg
      121 taacattgct tccccttttt gtgttgcttg ccgcctgtag cagcaagcca aaacctactg
      181 agactgaaac gaccaccgga acgccgtctg gcggcttcct gcttgagccg cagcacaatg
      241 tgatgcagat gggcggtgac ttcgccaata acccgaatgc ccagcagttc atcgacaaaa
      301 tggtgaacaa acacggtttc gatcgtcagc agttgcagga aattctctcc caggcgaaac
      361 gtctggattc ggtactgcgg ctgatggata accaggcacc aaccacatcg gtgaaaccgc
      421 catcaggtcc gaacggcgca tggctgcgtt atcgcaaaaa atttattacg ccggacaacg
      481 tgcagaacgg tgtggttttc tggaatcagt atgaagatgc gttgaatcgc gcgtggcagg
      541 tgtatggtgt accgccggaa attatcgtcg ggattatcgg cgttgaaacc cgctgggggc
      601 gcgtgatggg gaaaactcgc attctcgatg cgctggcaac gctgtcattt aattaccctc
      661 gccgcgcgga atatttttca ggtgagctgg aaaccttcct gctgatggcg cgcgacgagc
      721 aggacgatcc gctcaatctg aaaggttcct ttgcaggtgc gatgggttac ggtcagttta
      781 tgccatcgtc ttacaaacaa tatgcggtgg atttcagcgg tgacgggcat atcaacctgt
      841 gggatccggt tgatgccatc ggtagtgtag cgaactattt caaagcgcac ggctgggtga
      901 aaggcgagca ggtggcggtg atggcaaacg gtcaggcgcc aggcttaccg aatggcttca
      961 aaaccaggta cagcatttca caacttgccg cagctggact aacgccgcag cagtcgctgg
     1021 ggaatcatca gcaggccagc ttgctgcgtc tggatgttgg cactggttac cagtactggt
     1081 atggtctgcc gaacttctac actattaccc gttataacca cagcactcat tacgcaatgg
     1141 cggtctggca gttaggacag gccgtggcgc tggcgcgcgt gcagtaacta tttgcaagag
     1201 ggaggttaaa cttccctctg ttatcttact gtttattcga atggcgtaat tgcttgattt
     1261 ttttcgacag cgatatcgca tatcccaggc agacaacgcc taacgcagcc tgaccgaagt
     1321 caaaaggttc aagatagcca gaagataaaa ctttacatat cacggaaatc agtgcgctga
     1381 tgccgccaat gatgacccac atacgcattt gtgtggtttg tttgagcacg ttttgtgggg
     1441 tgttatccgt tttctttatt gccattgcta ttctctccct gtgaatacat atcaatgtga
     1501 acgcggcatg gtagcgcttt ttctctaccg actggccgca acagtctttt ttcggaacgc
     1561 gactcgtaaa atgaatcgct cgtccccatc ttttgcggga aagtgctatc ttgtccggca
     1621 taaatttttg actgacagag gttgtgatga ctgaaagtga actgatgcag ttaagcgaac
     1681 aggttgggct ggcgctgaaa gcccgtggcg caaccgtaac aactgctgag tcttgtaccg
     1741 gcggttgggt agcgaaagtg attaccgata ttgccggcag ttccgcctgg tttgaacgcg
     1801 gatttgtcac ctacagtaac gaagcaaaag cgcaaatgat cggtgtacgc gaagagacgc
     1861 tggcgcagca tggcgcggtg agcgaacctg tcgtggtgga aatggcgata ggcgcactga
     1921 aagcggctcg tgctgattac gccgtgtcta ttagtggtat cgccgggccg gatggcggca
     1981 gtgaagagaa gcctgtcggc accgtctggt ttgcttttgc cactgcccgt ggtgaaggca
     2041 ttacccggcg ggaatgcttc agcggtgacc gtgatgcggt gcgtcgtcag gctactgcgt
     2101 atgcattgca gaccttgtgg caacaatttc tacaaaatac ttgatactgt atgagcatac
     2161 agtataattg cttcaacaga acatattgac tatccggtat tacccggcat gacaggagta
     2221 aaaatggcta tcgacgaaaa caaacagaaa gcgttggcgg cagcactggg ccagattgag
     2281 aaacaatttg gtaaaggctc catcatgcgc ctgggtgaag accgttccat ggatgtggaa
     2341 accatctcta ccggttcgct ttcactggat atcgcgcttg gggcaggtgg tctgccgatg
     2401 ggccgtatcg tcgaaatcta cggaccagaa tcttccggta aaaccacgct gacgttgcag
     2461 gtgatcgccg cagcgcagcg cgaaggtaaa acctgtgcgt ttatcgatgc tgaacacgcg
     2521 ctggacccaa tctacgcacg taaactgggc gtcgatatcg acaacctgct gtgctcccag
     2581 ccggacactg gcgagcaggc actggaaatc tgtgacgccc tggcacgttc tggtgcagta
     2641 gacgttatcg tcgttgactc cgtggcggca ctgacgccga aagcggaaat cgaaggtgaa
     2701 atcggcgact ctcacatggg ccttgcggca cgtatgatga gccaggcgat gcgtaagctg
     2761 gcgggtaacc tgaagcagtc caacacgctg ctgatcttca tcaaccagat ccgtatgaaa
     2821 attggtgtga tgttcggtaa cccggaaacc actaccggtg gtaacgcgct gaaattctac
     2881 gcctctgttc gtctcgacat ccgtcgtatc ggcgcggtga aagagggcga aaacgtggtg
     2941 ggtagcgaaa cccgcgtgaa agtggtgaag aacaaaatcg ctgcgccgtt taaacaggct
     3001 gaattccaga tcctctacgg cgaaggtatc aacttctacg gcgagctggt tgacctgggc
     3061 gtgaaagaga agcttatcga gaaagcaggc gcgtggtaca gctacaaagg tgagaagatc
     3121 ggtcagggta aagcgaatgc gactgcctgg ctgaaagata acccagaaac cgcgaaagag
     3181 atcgagaaga aagtacgtga gttgctgctg agcaacccga actcaacgcc ggatttctct
     3241 gtagatgaca gcgaaggcgt agcagaaact aacgaagatt tttaatcgtc ttgtttgata
     3301 cacaagggtc gcatctgcgg cccttttgct tttttaagtt gtaaggatat gccatgacag
     3361 aatcaacatc ccgtcgcccg gcatatgctc gcctgttgga tcgtgcggta cgcattctgg
     3421 cggtgcgcga tcacagtgag caagaactgc gacgtaaact cgcggcaccg attatgggca
     3481 aaaatggccc ggaagagatt gatgccacgg cagaagatta cgagcgcgtt attgcctggt
     3541 gccatgaaca tggctatctt gatgacagcc gatttgttgc gcgctttatc gccagccgta
     3601 gccgcaaagg ttatggacct gcgcgtattt gtcaggaact gaatcagaaa ggtatttccc
     3661 gcgaagcgac agaaaaagcg atgcgtgaat gtgacatcga ctggtgcgca ctggcgcgcg
     3721 atcaggcgac gcgaaaatat ggcgaacctt tgccaactgt cttttcagaa aaagttaaga
     3781 tccagcgttt tctgctctat cgtggctatc tgatggaaga tatccaggat atttggcgaa
     3841 attttgccga ctgaacgcat acgggatttt acttcccagt caagaaaact tatcttattc
     3901 ccacttttca gttaccagcc gggcggttaa cgacacgcaa cggctggtag cgatatttcg
     3961 ttagcttgat ttcaggataa ttatgagcaa gagcaccgct gagatccgtc aggcgtttct
     4021 cgactttttc catagtaagg gacatcaggt agttgccagc agctccctgg taccccataa
     4081 cgacccaact ctgttgttta ccaacgccgg gatgaaccag ttcaaggatg tgttccttgg
     4141 gctcgacaag cgtaattatt cccgcgctac cacttcccaa cgctgcgtgc gtgcgggtgg
     4201 taaacacaac gatctggaaa acgtcggtta caccgcgcgt caccatacct tcttcgaaat
     4261 gctgggcaac ttcagcttcg gcgactattt caaacacgat gccattcagt ttgcatggga
     4321 actgctgacc agcgaaaaat ggtttgccct gccgaaagag cgtctgtggg ttaccgtcta
     4381 tgaaagcgac gacgaagcct acgaaatctg ggaaaaagaa gtagggatcc cgcgcgaacg
     4441 tattattcgc atcggcgata acaaaggtgc gccatacgca tctgacaact tctggcagat
     4501 gggtgacact ggtccgtgcg gcccgtgcac cgaaatcttc tacgatcacg gcgaccacat
     4561 ttggggcggc cctccgggaa gtccggaaga agacggcgac cgctacattg agatctggaa
     4621 catcgtcttc atgcagttca accgccaggc cgatggcacg atggaaccgc tgccgaaacc
     4681 gtctgtagat accggtatgg gtctggaacg tattgctgcg gtgctgcaac acgttaactc
     4741 taactatgac atcgacctgt tccgcacgtt gatccaggcg gtagcgaaag tcactggcgc
     4801 gaccgatctg agcaataaat cgctgcgcgt aatcgctgac cacattcgtt cttgtgcgtt
     4861 cctgatcgcg gatggcgtaa tgccgtccaa tgaaaaccgt ggttatgtac tgcgtcgtat
     4921 cattcgtcgc gcagtgcgtc acggtaatat gctcggcgcg aaagaaacct tcttctacaa
     4981 actggttggt ccgctaatcg acgttatggg ctctgcgggt gaagacctga aacgccagca
     5041 ggcgcaggtt gagcaggtgc tgaagactga agaagagcag tttgcccgta ctctggagcg
     5101 cggtctggcg ctgctggatg aagagctggc aaaactttct ggtgatacgc tggatggtga
     5161 aactgctttc cgtctgtacg acacctacgg cttcccggtt gacctgacgg ctgacgtttg
     5221 ccgtgagcgc aacatcaaag ttgacgaagc tggttttgac gcagcaatgg aagagcagcg
     5281 tcgtcgtgcg cgcgaagcca gcggctttgg tgcagattac aacgcaatga tccgtgttga
     5341 cagtgcatct gaatttaaag gctatgacca tctggaactg aacggcaaag tgactgcgct
     5401 gtttgttgat ggtaaatcgg ttgatgccat caatgcaggc caggaagctg tggtcgtgct
     5461 ggatcaaacg ccattctatg cggaatccgg cggtcaggtt ggtgataaag gcgaactgaa
     5521 aggcgctaac ttctccttcg cggtggaaga tacgcagaaa tacggccagg cgattggtca
     5581 catcggtaaa cttgctgtgg gttctctgaa agtgggcgac gctgtgcagg ctgatgttga
     5641 tgaggctcgt cgcgcccgta ttcgtctgaa tcactccgca acgcacctga tgcacgctgc
     5701 gctgcgccag gttctgggta ctcatgtatc gcagaaaggt tcactggtta acgacaaagt
     5761 gctgcgcttc gacttctcac acaacgaagc gatgaaacca gaagagattc gtgcggtcga
     5821 agacctggtg aacgcgcaga ttcgccgtaa cttgccgatc gaaaccaaca tcatggatct
     5881 cgaagcggcg aaagcgaaag gtgcgatggc gctgtttggc gagaagtatg atgagcgcgt
     5941 acgcgtgctg agcatgggtg atttctccac cgagttgtgt ggcggtactc acgccagccg
     6001 cactggtgat attggtctgt tccgcatcat ctctgaatcg ggtactgctg caggcgttcg
     6061 tcgtatcgaa gcggtaaccg gagaaggcgc tatcgccacc gttcatgcag acagcgatcg
     6121 cttaagcgaa gtcgcgcatc tgctgaaagg cgatagcaat aatctggctg ataaagtgcg
     6181 ttcagtactg gaacgtacgc gtcagctgga aaaagagtta caacagctta aagaacaagc
     6241 tgccgcacag gagagcgcaa atctttccag taaggcaatt gatgttaatg gtgttaagct
     6301 gttggttagc gagcttagcg gtgttgagcc gaaaatgttg cgtaccatgg ttgacgattt
     6361 aaaaaatcag ctggggtcga caattatcgt gctggcaacg gtagccgaag gtaaggtttc
     6421 tctgattgca ggcgtatcta aggacgtcac agatcgtgtg aaagcagggg aactgattgg
     6481 tatggtcgct cagcaggtgg gcggcaaggg tggtggacgt cctgacatgg cgcaagccgg
     6541 tggtacggat gctgcggcct tacctgcagc gttagccagt gtgaaaggct gggtcagcgc
     6601 gaaattgcaa taatataagc gtcaggcaat gccgtggact cgcttcacgg cattcgcatt
     6661 aacgctatcg acaacgataa agtcaggttg aagttgtgta tatcggctaa acttaggttt
     6721 aacagaatgt aatgccatga ctgcttagat gtaatgtgtt tgtcattgct tactttttgg
     6781 cgttatatga tggataatgc cgggatacag agagacccga ctcttttaat ctttcaagga
     6841 gcaaagaatg ctgattctga ctcgtcgagt tggtgagacc ctcatgattg gggatgaggt
     6901 caccgtgaca gttttagggg taaagggcaa ccaggtacgt attggcgtaa atgccccgaa
     6961 ggaagtttct gttcaccgtg aagagatcta ccagcgtatc caggctgaaa aatcccagca
     7021 gtccagttac taatctttcc gcgtctcatc tttatcggtg agacgcaccc tcaagatttc
     7081 tctttcactc cattgtcttt tcgctttact cccgttcatt caacttaagt ctccattttt
     7141 ttgcattact gctatctgtc agacctccgt ttttctgttg ataaaacact ctttttgacg
     7201 tttttacaga ctaattgagc gtgaagtgtg caaacgataa aagtgtggga aaaattgttt
     7261 gacttataag tctcagaaag taatatgtgc gccacgcagc gacgatgagc gataaacaag
     7321 ttcttcgaag cactcgtaag aggcgtgtgg tgaggtggcc gagaggctga aggcgctccc
     7381 ctgctaaggg agtatgcggt caaaagctgc atccggggtt cgaatccccg cctcaccgcc
     7441 atttgcatcc gtagctcagc tggatagagt actcggctac gaaccgagcg gtcggaggtt
     7501 cgaatcctcc cggatgcacc atctcttact tgatatggct ttagtagcga tatcaatatc
     7561 agcagtaaaa taaattttcc cgatgcatcc gtagctcagc tggatagagt actcggctac
     7621 gaaccgagcg gtcggaggtt cgaatcctcc cggatgcacc atattctccg taaccttcag
     7681 caatgaaggt atcgaagagg tagcggtatt aaccgcaggc accagggagg ataacgttgc
     7741 gccagcagcg gcccgaaggg cgaacgccag tgagtcatcc tcccggatgc accatattat
     7801 ccaaaaaatt tccttcaaaa atatctcatt tctaaataac tactttatcc atttcctgaa
     7861 ttttcttctc cttctccatc agcgacaaaa tcaagcaatt acgctaaagt agcgtcatat
     7921 ttttcgactt gcgagaagac catgtacgag cgttatgcag gtttaatttt tgatatggat
     7981 ggcaccatcc tggataccga gcctacgcac cgtaaagcgt ggcgcgaagt attagggcac
     8041 tacggtcttc agtacgatgt tcaggcgatg attgcgctta atggctcgcc cacctggcgt
     8101 attgctcagg cgattattga gctgaatcag gccgatctcg acccgcatgc gttagcgcgt
     8161 gaaaaaacag aagcagtaag aagtatgctg ctggatagtg tcgagccgct tcctcttgtt
     8221 gaagtggtga aaagctggca tggtcgtcgc ccaatggctg taggaacggg gagtgaaagc
     8281 gccatcgctg aggcattgct ggcgcacctg ggattacgcc gttattttga cgccgtcgtc
     8341 gctgccgatc acgtcaaaca ccataaaccc gcgccagaca catttttgtt gtgcgcgcag
     8401 cgtatgggcg tgcaaccgac gcagtgtgtg gtctttgaag atgccgattt cggtattcag
     8461 gcggcccgtg cagcaggcat ggacgccgtg gatgttcgct tgctgtgagt gaagcgttat
     8521 cgcttttctc gttgtttgcc agtagttttc tcagcgctac actattaccc ggcaactccg
     8581 aagtcgtgct ggtggcaatg ttgctttccg gagtgagtca tccctgggtt ttagttttaa
     8641 cagcaacaat gggtaatagc cttggagggt taactaacgt tatccttggg cgtttctttc
     8701 cattgcgtaa aacatcgcgc tggcaagaga aagctaccgg ctggctgaaa cgctatggtg
     8761 cagtcacgct attattaagc tggatgcccg tggttggcga tttactgtgt ctgttagcgg
     8821 gatggatgcg catctcgtgg ggaccggtaa tctttttttt gtgccttggt aaagcgttac
     8881 gctatgttgc agttgcagca gctaccgttc agggcatgat gtggtggcac taattgtagg
     8941 cctgcacata tggtcaccat tacagttatg ctaattaaaa cgattttgac aggcgggagg
     9001 tcaatttgat cccggacgta tcacaggcgc tggcctggct ggaaaaacat cctcaggcgt
     9061 taaaggggat acagcgtggg ctggagcgcg aaactttgcg tgttaatgct gatggcacac
     9121 tggcaacaac aggtcatcct gaagcattag gttctgcact gacccataaa tggattacta
     9181 ccgattttgc ggaagcattg ctggaattta ttacaccagt ggatggtgat attgaacata
     9241 tgctgacctt tatgcgcgat ctgcatcgct atacggcgcg caatatgggc gatgagcgga
     9301 tgtggccgtt aagtatgcca tgctacatcg cagaaggtca ggacatcgaa ctggcacagt
     9361 acggcacttc taacaccggt cgctttaaaa cgctgtaccg tgaagggctg aaaaatcgct
     9421 acggcgcgct gatgcaaacc atttccggcg tgcactataa tttctctttg ccaatggcat
     9481 tctggcaggc gaagtgcggt gatatctcgg gcgctgatgc caaagagaaa atttctgcgg
     9541 gttatttccg cgttatccgt aactactatc gcttcggttg ggtcattcct tatctgtttg
     9601 gtgcgtctcc agcgatttgt tcttctttct tgcaaggaaa accaacgtcg ctgccgtttg
     9661 agaaaaccga gtgcggtatg tattacctgc cgtatgcgac ctctcttcgt ttgagcgatc
     9721 tcggctatac caataaatcg caaagcaatc ttggtatcac cttcaatgat ctctacgagt
     9781 acgtggcggg ccttaaacag gcaatcaaaa cgccatcgga agagtacgcg aagattggta
     9841 ttgagaaaga cggtaagcgg ctgcaaatca acagcaatgt gctgcagatt gaaaacgaac
     9901 tgtacgcgcc gattcgtcca aaacgcgtta cccgcagtgg tgagtcgcct tctgatgcgc
     9961 tgttacgtgg cggcattgaa tatattgaag tccgctcgct ggacatcaac ccgttctcgc
    10021 cgattggtgt agatgaacaa caggtgcgat tcctcgacct gtttatggtc tggtgtgcgc
    10081 tggctgacgc accggaaatg agcagtagcg aacttgcctg tacacgcgtt aactggaacc
    10141 gggtgatcct cgaaggtcgc aaaccgggcc tgacgctggg tatcggctgc gaaaccgcgc
    10201 agttcccgtt accgcaggtg ggtaaagatc tgttccgcga tctgaaacgc gtcgcgcaaa
    10261 cgctggatag cattaacggc ggcgaagcgt atcagaaagt gtgtgatgaa ctggtcgcct
    10321 gcttcgataa tcccgatctg actttctctg cccgtatctt aaggtctatg attgatactg
    10381 gtattggcgg aacaggcaaa gcgtttgctg aagcgtaccg taatctgctg cgtgaagagc
    10441 cgctggaaat tctgcgcgaa gaggattttg tcgccgagcg cgaggcgtct gaacgccgtc
    10501 agcaggaaat ggaagccgct gataccgaac cgtttgcggt gtggctggaa aagcacgcct
    10561 gacagaaaag aaaaaggcca ctcgtgagtg gccaaaattt catctctgaa ttcagggatg
    10621 atgataacaa atgcgcgtct ttcatatact cagactcgcc tgggaacaaa gagttcagaa
    10681 aattttaaaa aaaattaccg gaggtggcta aatgccgttg ttagatagct tcacagtcga
    10741 tcatacccgg atggaagcgc ctgcagttcg ggtggcgaaa acaatgaaca ccccgcatgg
    10801 cgacgcaatc accgtgttcg atctgcgctt ctgcgtgccg aacaaagaag tgatgccaga
    10861 aagagggatc cataccctgg agcacctgtt tgctggtttt atgcgtaacc atcttaacgg
    10921 taatggcgta gagattatcg atatctcgcc aatgggctgc cgcaccggtt tttatatgag
    10981 tctgattggt acgccagatg agcagcgtgt cgctgatgcc tggaaagcgg caatggaaga
    11041 cgtgctgaaa gtgcaggatc agaatcagat cccggaactg aacgtctacc agtgtggcac
    11101 ttaccagatg cactcgttgc aggaagcgca ggatattgcg cgtaacattc tggaacgtga
    11161 cgtgcgcatc aacagcaacg aagaactggc gctgccgaaa gagaagttgc aggaactgca
    11221 catttagtca gtttaagcag aagaggccgg gctggtgaaa acaccgcccg gcttttgtta
    11281 cctgtcagtc atccgatatt ccacgctatg tgcttcatca ggttgaggaa cttccagcct
    11341 tgaggccatc cgcatcacga ccgcttcagg aactgcatac tcgcgtctgg cattttgctg
    11401 gttccattgc gcccatggca cttccagata aacgattttt actcgtgcgc cgtaagccgt
    11461 aaacagactg atgagctggc tgcgcagctg gcgggtaatg ttcgttgcat tccagatgaa
    11521 cggctttttc tggcgtaaaa aaacgcgcgc ctcttctttt gcctgttgca caatacgccc
    11581 ggtggcggtt ttgtcgtccg gactggcatt gattcgccgc cgcatatcat caaggctgat
    11641 aacgtccatt ccctggcact gttcactgat atagcgatct ttgcccatac ccggtaagcc
    11701 acacagcaaa ataacctcaa aaggttcagc ctcccaggga acaaaatcag gagaactttg
    11761 ctcatgagtc aggtagtgcc agcgtgcgga atcagaaacg aacgggcgca tttttcccca
    11821 gcattgttgc tcatggcaaa acagctcgaa cagatcgatg cgttccagca ttgattgctg
    11881 atccgggctt tggcgaccaa gcagatcggc acgagcaagc aaagcgagca gacgcgtgtc
    11941 gatgcgcatt gcagccgtga gcaacaaacg ctccggttcc ggacgttcca gtaaccatag
    12001 cggtaaccca tgcagacgca ccagtgcaac aatttgctcg cgcagcacga acggcgttgg
    12061 gatatcgcgc cataaaattt gtcgagccgt cagttcgccc cgacgagcat gaccaggaga
    12121 ctgaatacgt ccgttttcct gcacggtagt gctgcgcttt tcaacatcat gcaataacgc
    12181 cgccgcccat aaaacttcct gctgttgagc ggggagttgc tggaattctg gcagagtaat
    12241 gagggcgtta agcaccattt cggtatgtac gccgacgtcg ccttcaccat gatgctcagg
    12301 gtcttgtggc gtgtgatgca tctcctcaac ccagctaaaa cgctgacgta gcgccgacca
    12361 gcgtttatcg tcggtaagtt gccaaatcat gactgttctc cataaacgaa ctcatgggcc
    12421 atccgcgccc gttgccagtg gcgtttccag tgcacagtgg ttttgacatg atttttgcgc
    12481 acatacttga agacgttatg cgaaaagtca gcgacagaaa acgcatcatg atttcggctg
    12541 acaatgcctt ccagcgtaca gggttgacag gtttgcgtat cccagggatc gaatgtgccg
    12601 cgcgcattgg tcagcgccag aaaatcacgt tgccagcttt tttcatcatt ccccggctgc
    12661 gggccggaga tttcgggaac gcaggggaaa tcaaacagag cggcgtaaaa ctgcacctct
    12721 tcccagctca gccacatatc ctggcagcga acggcaaaca ggtaaaagtc ctgttccagc
    12781 gcgcggtatt caatggaatg aacggcgtag agattttcac caaacaactc caggttgccg
    12841 agatcgtttt tcagcagttg ccagcgttgg cgaattttat aggtccatgc cgactgcgta
    12901 ggcgtggcat gggagcgggc aaacacgccg tagcggttaa ggcagttatt ttcgccatcg
    12961 agtttttctg tgtgcaccaa ttgcgtgatg gtctgtaaat cctgccagta atcggtgtta
    13021 atgcggtcat cgctggtggt gcctggagag aaggggtaat gccaggttct cccgtattta
    13081 cgttgggtat tcataataat ccattatttt gtgtgtaata aatgtacgcc cgcgagggtg
    13141 cgagttttgg ggttaaaaga aaaatgtgag tatcacccgc gaaagcgagg gcgaaggaaa
    13201 aagagaatta acaacctgag cgaatgctca gaaccggcta atggtgaatg gaagagtttg
    13261 gggcggcgaa aagatacacc gaatgagtgt ataaaacaac cgtttgcaga atgagcaaat
    13321 aaaaagggag ccgcagctcc ctttggcaac cttagtgcgc accgcctccg ccgccacctg
    13381 cgccaaatgg cggtttagca aaccacacca gccccagcag gacgaggaat atcccggctg
    13441 acatccagaa gatctcattg gcggaaataa tcagcccctg attggttatc tgctgggcaa
    13501 tccagcctga tgcctgctgt tgcgtcatac caagcccttc cagttgactg tacatcgcct
    13561 gagcattcgg gttgaacggg tttaccgact cagtcaactg tgcatggtgc agcgactcgc
    13621 ggttggtcca catggtcgtg gttatcgacg tgccgataga ccccgccagc gttcgcgtaa
    13681 agttggagag gctcgatgcc gccgccagtc gttccggtgg caaaccggac agcgtaatgg
    13741 tggtcagcgg cataaagaag caggccaccg caaaaccctg gataaactgt ggccaggccg
    13801 acgcgccaaa atccataccc ggttcaaagg tataggcacg ccagtagaag cagacggcat
    13861 acataataaa gctgaaggtt accagtcggc gcatatccag tttatgcgcg aagcggccga
    13921 taatcggcga caggatcacc ggaataatcc ctaccggcgc agaggccagc cctgcccacg
    13981 tcgccgtgta accgtagacc tcctgcaaca actgcggcag cagaacaatc gcgccgaagt
    14041 agagcatata cgcgaggctg atacacaagc agccgatggt gaagttgcgc gacttaaaca
    14101 acgacagatc gactatcggg ttatcgtcgg tcagctccca gacaatcagg aagcagatag
    14161 ccaccaccgc caccacggtg aggatgataa tttcctgtga tgagaaccag tccagctctt
    14221 taccgcggtc gagcataatc tgcaggctgc cgataccaat aaccagcagt gccagcccca
    14281 cggcatcaat ccgccgccgt tcggtgcggg tttcacgtcc gcgcagagtt tgcagtgtca
    14341 tcaacaccac cgccacgcca atcggcacgt tgatgaagaa tatccagccc cagtggtaat
    14401 tatcactgat ataaccgccg aggatcgggc cgcaaattgg cgcaacaatc accgtcatcg
    14461 accacaacgc cagcgcgatc gagcgtttgg ctggcgggta gttattcagc aatagacttt
    14521 gcgaaagcgg aattaacggc ccggcgacaa tcccctgaat cacgcggaag aagatcagca
    14581 tattcaggct gctggagaca ccacacgccc acgacgcaat agcaaaggcg atggtggacc
    14641 agaggaacag tttcacttcc ccgacgcgct ttgccagcca gccggtaagc gggatcgaga
    14701 tggcattcgc caccccgaaa gaagtgatta cccacgttcc ctggctgagc gatgagccca
    14761 gattcccggc gatagtgggg atcgccacgt tagcaatggt ggagtccagc acctgcatga
    14821 atgtcgccag tgacagcgca atcgtcataa tgaccagttg tgcgccttcc agcggttttt
    14881 gctgttgcat cacacgcacc tctggattag ccagcgttag cttttacgat atcgtcgatc
    14941 agtttattga caggtgccag gctgatttca cgcgcggtgc ttaccgcgac cggtgtggaa
    15001 cgtactttgt ttgccagtac ctgaccgtca cggttagtgg tattgacact caccagcgtg
    15061 gataaaccga tacgcagcgg gtattgctcc agctgtttct ggtccagttc aatacgcaca
    15121 ggcagacgct gaacgacttt gatccagtta ccggtggcgt tttgcgccgg aagcagtgag
    15181 aacgcgctac ctgtgcccat atccagacca accactttac cggtgtattt cacatcatcg
    15241 ccgtaaatat cggtggtgat ggtgaccggt tgaccgatac gcatattggc aatctgcgtc
    15301 tctttaaagt tggcatccac ccacatattg gtggctggaa cgaccgccat cagcggcgtc
    15361 gttgggctaa tttgcgcccc aggctgtacc gcgcggcggg agacataacc ggtcatcgga
    15421 ctgacaatac gggtacgctc cagcgccagc caggcgttac gtacttcggt ggcagcctgt
    15481 tgtacggcag gctgatcttc cagtttcgtc cccagaatca tcgcctgatt ggcattgtat
    15541 tgttgaatag cgacgtccag ttgcgcctgg gcactggtga cggcgtcgcg ggcgtgttgc
    15601 agctcttcgc gaccaatcag gttggcattg cccagcggca cacggcggtt gtagtcgctt
    15661 tgtgctttcg cgagggcgat tttttgcacc tcaatattcg cctgcaactg tttgctgtta
    15721 atcatcagct ggtgggtttg gcgaacgctg gaagccagtg cagttttggc tttttcaaac
    15781 gcctggcgag catctgtcgg gtcgagagtg accagcacgt cgccttcttt tacaaaatcg
    15841 gtgttatcgg cccagacttt cgtcacgctg ccagacacct gagacataat ttgaatctga
    15901 ttccctgcca cgtatgcgtc atcggtttct tcgaaatgac gcagtaccaa aaaccaataa
    15961 atccctatcg ctacggcaat aattataaag agcaaggtga gaaggaggag cagacgctta
    16021 cgtttgccgc tcttctttac cggttgctgc ggggtttgag tctccgcatt tgcgctcata
    16081 ttgttctcca cgatcttctt aactcatcgg ctgagccgac ctgcttgcca aaaaggccag
    16141 catgttgcga tgctggccgg ttattttttc ttttataaat ctggattttt gagcgagatg
    16201 acgcgttagc tcatcgcttc gagaaccaca ccgccctgtt ccatctggtc gagacgggag
    16261 agcaatttgc gggtgatttg ctcgagctgg tctttttctg ttgtgctgag cgcggaccag
    16321 agttgatgca ggcagttatg ctgcggcggt aaaacctcgc gcaaaaactc gtgacctttt
    16381 tccgttaatt gcagatgcag gcagcggcga tcgttatcgc tttcacgacg ttcgatccaa
    16441 ccgcgttttt ccagttcatc ggcaatacgc gtcgcgttgg tacgggatga tccaagagca
    16501 caactcaatt cagaaggctg aatactgtgg ttttcctgag actccagcgt aatcaacgcc
    16561 ataaacaacg tctcgttaat cccctgagcc ttcagcattt tattgcggtt ttccagcagc
    16621 ttgctttgca tatgcatgca aagacgggtc agaaggatct cctgataagg aaaatcttcg
    16681 tggcggctgg cgcgaaattt tagcatttgt tcaatgggcg taaacgaact atccatttgg
    16741 gtatgacctc attaatttca gccgatatag taacgacagt gacaaataaa gtaaatgtat
    16801 tgttttagaa aaatgattct tgtggattat ataatcgcca tcactttcca ggcgagccca
    16861 taggccagcg cactaagcag tgttgggatg ataatgctgc gtgttttata gaaactggca
    16921 cccagtaccg cgaagccgac cagtgtgggc acgaaacggc gtgtatcgtg catcacttct
    16981 ggtgcggtag agacaaccag cagagcgcat atcgaggcga tgccaatggt gtcgagcaaa
    17041 atacctaccg cgccacgttt ggttgggcgg gcattaccca cacgcaggcg cagcggcaga
    17101 tagcggaagc aataattcgc cgcgccaacc agtaacccaa gcagcagaac ctcatagctc
    17161 atcgggcgct ccttgccaga atgcctggat taacgcagtg aggcagccac agacaatgcc
    17221 tgccagaatg gcgacgggaa tagaaaatag cgttacgcct gcaagggcac caactaacgc
    17281 tgcggtaacg caaagagatt gtttgcgctg gaaagaggcg agcaggaaac tcataaagag
    17341 tgccggaagc ataaaaccta atgctgcttc aacggcggga taaccttgca gcaagccgct
    17401 gccggagaat gcccctatta ccgtaccaaa tacccacgat gaccatgaac tgaaggcaat
    17461 gccgatcatc cagttttcgc tccagcggcg attattgcgt accagttttg cggttgcggc
    17521 ggcaaaaacc tcatccgtca ggccaaacgc ccacagggcg gttttcgatt tttgcagacg
    17581 ctgaataata cggctacgca gtgacgggcc atacaaaaca tggcgaacat ccattgccat
    17641 gacggtcagt gcagcaaccc acaaactact cccggctgcc agcatcgcgg taatgacgaa
    17701 ctggctcgcg cctgcataaa tgatgcagga gaaaaaaacg ctttcgagag gagagaatcc
    17761 cagacgggtc gcattcagac cgaacgcaaa ggccaccgga atataactaa taacaatcgg
    17821 taaactgtct ttgcatcctt ccatgaaggt cgccgaacca ggagcaggct gtggagtagg
    17881 gctttccata gagtgtacgc ttaacgattg ttaatattat aaatagactg aatgaatatc
    17941 ttaaccttat cagactgatg ggcttcttaa cacccttata agtgtaaagc cacgaaaacg
    18001 gttgctgatt gcttaatttg cggcttcctg acgatgaaaa cctcgccacc agaccagtaa
    18061 gttaaccagg gcaatcgtcg cgccagccag acacacgcca gcccaactgc catgttgcca
    18121 ggctgaggct gaaattagcg aaccagcggc accgccaata aagtagctgg tcatgtaacc
    18181 tgcggtcagg cgattacgcg catcagggtg tattcgataa attaccgtct ggttagtgat
    18241 atgcacgccc tgcacggtga ggtccagcac caggatgccg ataatcagcg ccagtacgga
    18301 agtgtgacca aaccagattg ccagccatga aagtaacagc agaagcagac cgaaagttgt
    18361 tgtgtggtgc gatttgccct tgtcggcaaa accgcccgcc ggacgagcgc ccaacgctcc
    18421 ggcagctccc gcaagtccaa acagaccaat tacaccatcg ctgtagttaa aaggtggagc
    18481 ggcaagcaaa aaggccattg aggtccagag aatgctgaag ttggcaaagg tcaggcagcc
    18541 cagcaacgcg cgggtgcgca ggatcttatc gctgataaac atgctgaaaa cggaaccaag
    18601 caactgtggg tagttgaggt gggtttctga tttcatttgt ggcagaccac gccataatgc
    18661 cagcgccatc agtgccatta acaccgaggc aacccaaaag acggtgcgcc agccgccgag
    18721 gtttgccagc aatccggcaa cggtccgtgc gagtaagatc cccaatagca ggccgctcat
    18781 aatggtgcca accactttgc cgcgtttgtc cggtgaggcc agcgtcgctg ccagtggaac
    18841 cagaatttgt gccacgaccg agaataaacc ggttaatgcc gtaccgagga tcatcatcgc
    18901 cagtgactga ctgctggcag tgattaacat accgcccgct gccagtaagg tcatcgagac
    18961 aatcaggcgg cggcgttcaa acatatcacc gaggggaaca agaaacagca gacctgcggc
    19021 atagcccaac tgcgcggcgg taacaataaa gcccgccgaa ctggcggaaa gggaaaagtt
    19081 acgcgcgatg gtgtcgagca atggctgggc gtaatagtta ctggctaccg ccagaccggt
    19141 ggcgatagac atcagcacga tcagcgccgg gctaagctca tgattaggtt tagtcattat
    19201 ttttgttcat caggtgatga ttaagctgga aatcaataat agcggatgtt gatgaatatg
    19261 ggatgaaaga tagattgttg aaatgtgcgg tgtggtttgt aggtatgata agacgcgcca
    19321 gcgtcgcatc aggcattgtg tacagagcgg cggatgaggc aaaaacgcct tatccgcccg
    19381 aataaaaatt acttctgcgc tgccagcgct tcattcaccc agccatcgaa ctgctgctgg
    19441 tgggctttga tccaaccatc aacgtggccc tgaatatcgt cttctgaggc tttgccgtca
    19501 tgcatgatgg cgttctgggc gttaatatct gccactggta actgcataat ggcaaacagt
    19561 ttcgctgctg ccgggttttt ctcggcccag gctttgttgg caacgatatg catggtactg
    19621 accgggaagc cgtaattcgc gccattcggc agtttggtat tggcattttt atcgcccggc
    19681 agtgcggaga acggcacctg caaccagacc acatctttcc ccggtttcag ttcgttactc
    19741 acccagtacg gcgtccaggt gtagtaaaac accggcttac cctctttgta acgactgatg
    19801 gtgtcagcca tcatcgctgc gtagttcccc tgattatgcg tcacggtatt ggtcagtcca
    19861 tacgcggcaa gctggtggtt gatcgcacct tcgcagcccc agcctgggtt acagccggtt
    19921 aaatccgctt taccgtcgcc gttggtatcg aacagtttgg cgatcttcgg atctttaagt
    19981 tgcgcgatgt tggtaatttt atactggtcg gcggttttct tatcgatcag gtaaccttgt
    20041 gccgcgccgt taacaaatac cccttcacga taaaatttct tatcgccacc ggcagcttcg
    20101 tacatgttgt catgcagtgg cgtccagttc acggcggtga aggttgcatc gccggaagca
    20161 agcgaggtgt agccaacgtt gtaatccact tcgctgggtt tattgacggt ataacctaat
    20221 ttctccagcg cgcggctgac cagcagcgtc tggaaggttt cttcggtgat ggtgctttga
    20281 actggattaa ctgtaatgcc tttgcccggc aggtcggcag caaaagtttg tgtagagata
    20341 agcgtggcaa acgctgtcgc aaaaagtacg ctatgtcgca ttgttattcc tttttttggc
    20401 agggtgatat gcccggtgtt accgggcaag tgcagagtta cttaatgaat gggcgggtca
    20461 gcagaccgac agggccagtg gtgtaccagc gacggttgcc gcgactacgt gagtcgcgcc
    20521 caacggcctg cgtcaggcga tcgaggataa tggcgaggat cacaatcccg acgccgccaa
    20581 cggtggcaag ccccatatcc agacgaccga taccgcgaag taccatctga cccagcccgc
    20641 cgacggcaat catcgaagcg atgaccacca tagaaagggc cagcatcagc gtctggttaa
    20701 cgcccgccat aatggttggc atcgccagcg gtaactgaac tttgaacagc atctggctcg
    20761 ggctggcacc gaatgagcgc gaggcttcaa tcagatccgc cggaacctgg ttaattccca
    20821 gaatcgtcag acggataatc ggcggcagtg caaagatgat cgtcaccacc acgcccggca
    20881 cgttgccgat accaaacagc atgacgattg gcaccagata aacaaacgcg ggcgtggtct
    20941 gcatggcatc aagcagtgga cgaataattt tcgccgctcg cggacttctc gccagccaga
    21001 tccccaacgg caaacctatg acgatacaga acagcagggc ggttaacacc agcgccaggg
    21061 taaccattgc ctgtgaccag gcaccgattg cgccgatggc aatcagcgaa accagcgtcg
    21121 ccacgcccat tccgaccccg gaaatctgcc aggcgatgag agcgaaaacg ataatcgcca
    21181 ccggcgcggg catacccagc agcaattgct ggaaaccgtt gaggatgtaa tcaaccggaa
    21241 cgcgcacgcc ctgaaagacg ggacgaaaat gggtaacgac ccagtcgatc ccttcagtga
    21301 cccaactgtc gagcgggatc agagttttat ggaacggatc gagaatatta aaatgctcga
    21361 cgtttggcgc aggcgtactg gtcagccagt cagcaccacc gccgtcagtc ggtgcagcag
    21421 ccggtgtacc ccaggcgtct gcggattgtg cagcactgtc cgccgctggc gtggtatccc
    21481 acggattatt ttgatcagcc attatttacc ccctcacgat ctaaagcgcg cagcagcatt
    21541 cctttcgaaa tgatgccgac atactgttgg tcctcgtcga ccacgggcac cgcacagggt
    21601 gcctgtccga catgagagag caactcgcta agaggcgttt gtgcatcgac cgctaacggc
    21661 gcatcaatca gcgccgcatc aagaccttgc tgctgcgtta acgcggcttt aagcgaatcg
    21721 atagacacag cgccgacaaa cttattaccg cgttcgatga cgtagccata ttcgcgatct
    21781 tcatcctgca ataatttcag tgccgaacgc gggccgaagc cgggggtttt acgaattaag
    21841 ccgttcggtg tacgacgagc aatatctttc gcactgaaca cctggctaat atcgacgcca
    21901 cggaagaagg tacgaacata atcattcgcc ggattattaa gaatttcatc cggtgtacca
    21961 acctgcacca cttcaccatt ttgcataatg gcaattcggt caccaatacg catagcttca
    22021 tcgagatcgt gggaaataaa gacaatggtg cgctgatgtt tcgcctgtaa ttttaccagc
    22081 tcatcctgca tctcggtgcg aattaatgga tcgagtgccg agaaggcttc gtccattaat
    22141 aatatatccg gattaatcgc taacgcgcgg gctaatccca cacgttgacg catcccgcca
    22201 gagagttcat ccgggtagct gtgggcataa ttttccagcc cgacctgacg cagtgcatca
    22261 agggcttttt cccggcgttc ttcggcatta attccggcca gttccatacc gaacgcagta
    22321 ttgtccagca cggtcatatg cggcattaag gcaaaggact ggaagaccat cgcaatcttt
    22381 tttctgcgca cctcacggag ttcgacgtcg gatattttgg caatatccac accatcaatc
    22441 agcacttgcc cgcgggtggg ttcaatcagg cgattgagaa ggcgtaccat tgtggattta
    22501 cccgaaccgg ataatcccat gatgacaaat atctcgcctt cttcaatggc cagactggcg
    22561 tcttttacgc caagcgatag cccagttttt tccagaattt gttcttttga aagtccttgt
    22621 tcgatatatt tgaaagctcg
//
LOCUS       DAHHTG010000070        22598 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.070, whole genome shotgun sequence.
ACCESSION   DAHHTG010000070 DAHHTG010000000
VERSION     DAHHTG010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22598)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22598)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22598
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.070"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            358..1257
                     /gene="yegS"
                     /locus_tag="NBI74_002724"
     CDS             358..1257
                     /gene="yegS"
                     /locus_tag="NBI74_002724"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid kinase YegS"
                     /protein_id="HCD1933031.1"
                     /translation="MAEFPASLLILNGKSTDNLPLREAIMLLREEGMTIHVRITWEKG
                     DAARFVEEARKLGVATVIAGGGDGTINEVSTALIQCEGDDIPALGILPLGTANDFATS
                     VGIPEALDKALKLAIAGNAIAIDMAQVNKQTCFINMATGGFGTRITTETPEKLKAALG
                     GVSYIIHGLMRMDTLQPDRCEIRGENFHWQGDALVIGIGNGRQAGGGQQLCPNALIND
                     GLLQLRIFTGDEIIPTLVSTLKSDEDNPNIIEGASSWFDIQAPHEITFNLDGEPLSGQ
                     NFHIEILPAALRCRLPPDCPLLR"
     gene            complement(1339..2112)
                     /locus_tag="NBI74_002725"
     CDS             complement(1339..2112)
                     /locus_tag="NBI74_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCD1933032.1"
                     /translation="MNSFERRNKIIQLVNEQGTVLVQDLAGVFAASEATIRADLRFLE
                     QKGVVTRFHGGAAKIMSGNSETETQEVGFKERFQLASAPKNRIAQAAVKMIHEGMTVI
                     LDSGSTTMLIAEGLMTAKNITVITNSLPAAFALSENKDITLVVCGGTVRHKTRSMHGS
                     IAERSLQDINADLMFVGADGIDAVNGITTFNEGYSVSGAMVTAANKVIAVLDSSKFNR
                     RGFNQVLPIEKIDIIITDDAVSEVDKLALQKTRVKLITV"
     gene            complement(2218..3258)
                     /gene="gatD"
                     /locus_tag="NBI74_002726"
     CDS             complement(2218..3258)
                     /gene="gatD"
                     /locus_tag="NBI74_002726"
                     /EC_number="1.1.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HCD1933033.1"
                     /translation="MKSVVNDTDGIVRVAESVIPEIKHQDEVRVKIASSGLCGSDLPR
                     IFKNGAHYYPITLGHEFSGYIDAVGSGVDDLHPGDAVACVPLLPCFTCPECLKGFYSQ
                     CAKYDFIGSRRDGGFAEYIVVKRNNVFALPADMPIEDGAFIEPITVGLHAFHLAQGCE
                     NKNVIIIGAGTIGLLAIQCAVALGAKSVTAIDISSEKLALAKSFGAMQTFNSREMSAP
                     QIQGVLRELRFNQLILETAGVPQTVELAVEIAGPHAQLALVGTLHQDLHLTSATFGKI
                     LRKELTVIGSWMNYSSPWPGQEWETASRLLTERKLSLEPLIAHRGSFESFAQAVRDIA
                     RNAMPGKVLLIP"
     gene            complement(3306..4661)
                     /locus_tag="NBI74_002727"
     CDS             complement(3306..4661)
                     /locus_tag="NBI74_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HCD1933034.1"
                     /translation="MFSEVMRYILDLGPTVMLPIVIIIFSKILGMKAGDCFKAGLHIG
                     IGFVGIGLVIGLMLDSIGPAAKAMAENFDLNLHVVDVGWPGSSPMTWASQIALVAIPI
                     AILVNVAMLLTRMTRVVNVDIWNIWHMTFTGALLHLATGSWMIGMAGVVIHAAFVYKL
                     GDWFARDTRDFFELEGIAIPHGTSAYMGPIAVLVDAIIEKIPGVNRIKFSADDIQRKF
                     GPFGEPVTVGFVMGLIIGILAGYDVKGVLQLAVKTAAVMLLMPRVIKPIMDGLTPIAK
                     QARSRLQAKFGGQEFLIGLDPALLLGHTAVVSASLIFIPLTILIAVCVPGNQVLPFGD
                     LATIGFFVAMAVAVHRGNLFRTLISGVIIMSITLWIATQTIGLHTQLAANAGALKAGG
                     MVASMDQGGSPITWLLIQVFSPQNIPGFIVIGAIYLTGIFMTWRRARGFIKQEKAVLA
                     E"
     gene            complement(4665..4949)
                     /gene="gatB"
                     /locus_tag="NBI74_002728"
     CDS             complement(4665..4949)
                     /gene="gatB"
                     /locus_tag="NBI74_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIB"
                     /protein_id="HCD1933035.1"
                     /translation="MKRKIIVACGGAVATSTMAAEEIKELCQSHNIPVELIQCRVNEI
                     ETYMDGVHLICTTAKVERSFGDIPLVHGMPFVSGVGIEALQNKILTILQG"
     gene            complement(4980..5432)
                     /gene="gatA"
                     /locus_tag="NBI74_002729"
     CDS             complement(4980..5432)
                     /gene="gatA"
                     /locus_tag="NBI74_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIA"
                     /protein_id="HCD1933036.1"
                     /translation="MTNLFVRGGISFVDRSEVLTHIGNEMLAKGVVHDTWPQALIARE
                     AEFPTGIMLEQHAIAIPHCEAIHAKSSAIYLLRPTNKVLFQQADDDNDVAVSLVIALI
                     VENPQQQLKLLRCLFGKLQQPDIVETLITLPEPQLKEYFTKYVLDSDE"
     gene            complement(5442..6704)
                     /gene="gatZ"
                     /locus_tag="NBI74_002730"
     CDS             complement(5442..6704)
                     /gene="gatZ"
                     /locus_tag="NBI74_002730"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatZ"
                     /protein_id="HCD1933037.1"
                     /translation="MKTLIARHKAGEHIGICSVCSAHPLVIEAALAFDRNSTRKVLIE
                     ATSNQVNQFGGYTGMTPADFREFVFAIADKVGFARERIILGGDHLGPNCWQQENADAA
                     MEKSVELVKAYVRAGFSKIHLDASMSCADDSIPLAPETVAERAAVLCLAAESVATDCQ
                     REQLNYVIGTEVPVPGGEASAIQSVHITQVEDAANTLRTHQKAFIARGLAEALTRVIA
                     IVVQPGVEFDHSNIIHYQAQEAQALAQWIEKTKMVYEAHSTDYQTQAAYRELVRDHFA
                     ILKVGPALTFALREAIFALAQIEQELIAPENSSGCLAVIEEVMLDEPQYWKKYYRTGF
                     NDSLLDIRYSLSDRIRYYWPHSRIKNSVETMMVNLEGVDIPLGMISQYLPKQFERIQS
                     GELSAIPHQLIMDKIYDVLRAYRYGCAE"
     gene            complement(6733..7587)
                     /gene="gatY"
                     /locus_tag="NBI74_002731"
     CDS             complement(6733..7587)
                     /gene="gatY"
                     /locus_tag="NBI74_002731"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416599.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatY"
                     /protein_id="HCD1933038.1"
                     /translation="MYVVSTKQMLNNAQRGGYAVPAFNIHNLETMQVVVETAASMHAP
                     VIIAGTPGTFTHAGTENLMALVSAMAKQYHHPLAIHLDHHTKFDDIAQKVRSGVRSVM
                     IDASHLPFAQNISRVKEVVDFCHRFDVSVEAELGQLGGQEDDVQVNEADALYTNPVQA
                     REFAEATGIDSLAVAIGTAHGMYARAPALDFSRLENIRQWVNLPLVLHGASGLSTKDI
                     QQTIKLGICKINVATELKNAFSQALKNYLTEHPEATDPRDYLQSAKSAMRDVVSKVIA
                     DCGCEGRA"
     gene            complement(7814..8866)
                     /gene="fbaB"
                     /locus_tag="NBI74_002732"
     CDS             complement(7814..8866)
                     /gene="fbaB"
                     /locus_tag="NBI74_002732"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HCD1933039.1"
                     /translation="MTDIAQLLGKDADNLLQHRCMTIPSDQLYLPGHDYVDRVMIDNN
                     RPPAVLRNMQTLYNTGRLAGTGYLSILPVDQGVEHSAGASFAANPLYFDPKNIVELAI
                     EAGCNCVASTYGVLASVSRRYAHRIPFLVKLNHNETLSYPNTYDQTLYASVEQAFNMG
                     AVAVGATIYFGSEESRRQIEEISAAFERAHELGMVTVLWAYLRNSAFKKDGVDYHVSA
                     DLTGQANHLAATIGADIVKQKMAENNGGYKAINYGYTDDRVYSKLTSENPIDLVRYQL
                     ANCYMGRAGLINSGGAAGGETDLSDAVRTAVINKRAGGMGLILGRKAFKKSMADGVKL
                     INAVQDVYLDSKITIA"
     gene            9123..10400
                     /locus_tag="NBI74_002733"
     CDS             9123..10400
                     /locus_tag="NBI74_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCD1933040.1"
                     /translation="MKTTAKLSFMMFVEWFIWGAWFVPLWLWLSKSGFSAGEIGWSYA
                     CTAIAAILSPILVGSITDRFFSAQKVLAVLMFAGAVLMYFAAQQTTFAGFFPLLLAYS
                     LTYMPTIALTNSIAFANVPDVERDFPRIRVMGTIGWIASGLACGFLPQMLGYADISPT
                     NIPLLITAGSSALLGVFAFFLPDTPPKSTGKMDIKVMLGLDALILLRDKNFLVFFFCS
                     FLFAMPLAFYYIFANGYLTEVGMKNATGWMTLGQFSEIFFMLALPFFTKRFGIKKVLL
                     LGLVTAAIRYGFFIYGSADEYFTYALLFLGILLHGVSYDFYYVTAYIYVDKKAPVHMR
                     TAAQGLITLCCQGFGSLLGYRLGGVMMEKMFAFQEPVNGLTFNWAGMWTFGAVMIAII
                     AVLFMIFFRESDNEITAIKVDDRDIALTQGEVK"
     gene            10397..11401
                     /locus_tag="NBI74_002734"
     CDS             10397..11401
                     /locus_tag="NBI74_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HCD1933041.1"
                     /translation="MKTERILGALYGQALGDAMGMPSELWPRSRVKAHFGWIDRFLPG
                     PKENNAACYFNRAEFTDDTSMALCLADALLEREGKIDPDLIGRNILGWALRFDAFNKN
                     VLGPTSKIALNAIRDGKPVAELENNGVTNGAAMRVSPLGCLLPARDVDSFIDDVALAS
                     SPTHKSDLAVAGAVVIAWAISRAIDGESWSAIVDSLPSIARHAQQKRITTFSASLAAR
                     LEIALKIVRNADGTESASEQLYQVVGAGTSTIESVPCAIALVELAQTDPNRCAVLCAN
                     LGGDTDTIGAMATAICGALHGVNAIDPALKAELDAVNQLDFNRYATALAKYRQQREAI
                     "
     gene            11398..12363
                     /locus_tag="NBI74_002735"
     CDS             11398..12363
                     /locus_tag="NBI74_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HCD1933042.1"
                     /translation="MSGARLHTLLPELTTRQPVMVVGAAVIDVIADAYALPWRGCDIE
                     LKQQSVNVGGCALNIAVALKRLGIEAGNALPLGQGVWAEIIRNRMAKEGLISLIDNAE
                     GDNGWCLALVEPDGERTFMSFSGVENQWNREWLARLTVAPGSLLYFSGYQLASPCGEL
                     LVEWLEELQDVTPFIDFGPRIGDIPDALLARIMACRPLVSLNRQEAEIAAERFALSAE
                     ITTLGKQWQEKFAAPLIVRLDKEGAWYFSNDASGCIPAFPTQVVDTIGAGDSHAGGVL
                     AGLASGLPLADAVLLGNAVASWVVGHRGGDCAPTREELLLAHKNV"
     gene            complement(12337..13083)
                     /locus_tag="NBI74_002736"
     CDS             complement(12337..13083)
                     /locus_tag="NBI74_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCD1933043.1"
                     /translation="MEQAHTQLIAQLNERILAADNTPLYIKFAETVKNAVRSGVLEHG
                     NILPGERDLSQLTGVSRITVRKAMQSLEEEGVVTRSRGYGTQINNIFEYSLKEARGFS
                     QQVVLRGKKPDTLWVNKRVVKCPEDVAQQLAVEAGSDVFLLKRIRYVDEEAVSIEESW
                     VPAHLIHDVDAIGISLYDYFRSQHIYPQRTRSRVSARMPDAEFQSHIQLDSKIPVLVI
                     KQVALDQQQRPIEYSISHCRSDLYVFVCEE"
     gene            complement(13135..13953)
                     /locus_tag="NBI74_002737"
     CDS             complement(13135..13953)
                     /locus_tag="NBI74_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
                     /protein_id="HCD1933044.1"
                     /translation="MQLRITSRKKFTVLLCALGLISIVAIYPRQTVNFFYSTAIQIKD
                     YIHFYGYRPVKSFAIRIPASYTIHGIDVSRWQERIDWQRVAKMRDNGIRLQFAFIKAT
                     EGEKLVDPYFSRNWQLSRENGLLRGAYHYFSPSVSASVQARLFLQTVDFSQGDFPAVL
                     DVEERGKLSAKELRKRVSQWLKMVEKRTGKKPIIYSGAVFYHTNLAGYFNEYPWWVAH
                     YYQRRPDNDGMAWRFWQHSDRGQVDGINGPVDFNVFNGTEEELQGFVDGIKETP"
     gene            complement(14018..14818)
                     /gene="thiD"
                     /locus_tag="NBI74_002738"
     CDS             complement(14018..14818)
                     /gene="thiD"
                     /locus_tag="NBI74_002738"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HCD1933045.1"
                     /translation="MKRINALTIAGTDPSGGAGIQADLKTFSALGAYGCSVITALVAQ
                     NTRGVQSVYRIEPDFVAAQLDSVFSDVRIDTTKIGMLAETDIVEAVAERLQRYQIQNV
                     VLDTVMLAKSGDPLLSPSAVATLRSRLLPQVSLITPNLPEAAALLDAPHARTEQEMLE
                     QGRSLLAMGCGAVLMKGGHLDDEQSPDWLFTREGEQRFTAQRIMTKNTHGTGCTLSAA
                     LAALRPRHTNWADTVQEAKSWLSSALAQADTLEVGHGIGPVHHFHAWW"
     gene            complement(14815..15603)
                     /gene="thiM"
                     /locus_tag="NBI74_002739"
     CDS             complement(14815..15603)
                     /gene="thiM"
                     /locus_tag="NBI74_002739"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HCD1933046.1"
                     /translation="MQVDLLSSAQSAHALHLFHQHSPLVHCMTNDVVQTFTANTLLAL
                     GASPAMVIETEEASQFAAIASALLINVGTLTRPRAQAMSAAVEQATRSQTPWTLDPVA
                     VGALDYRRRFCLELLSHKPTAIRGNASEIMASAGVANGGRGVDTTDAAANAIPAAQTL
                     ARETGAIVVVTGEVDYVTDGHRIIGIHGGDPLMTKVVGTGCALSAVVAACCALPGDTL
                     ENIASACHWMKQAGERAVARSEGPGSFVPHFLDALWQLTQEVQA"
     regulatory      complement(15638..15734)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(15826..16098)
                     /gene="rcnR"
                     /locus_tag="NBI74_002740"
     CDS             complement(15826..16098)
                     /gene="rcnR"
                     /locus_tag="NBI74_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II)-binding transcriptional repressor
                     RcnR"
                     /protein_id="HCD1933047.1"
                     /translation="MSHTIRDKQKLKARASKIQGQVVALKKMLDEPHECAAVLQQIAA
                     IRGAVNGLMREVIKGHLTEHIVHQGDELKREEDLDVVLKVLDSYIK"
     gene            16219..17043
                     /gene="rcnA"
                     /locus_tag="NBI74_002741"
     CDS             16219..17043
                     /gene="rcnA"
                     /locus_tag="NBI74_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel/cobalt efflux protein RcnA"
                     /protein_id="HCD1933048.1"
                     /translation="MTEFTTLLQQGNAWFFIPSAILLGALHGLEPGHSKTMMAAFIIA
                     IKGTIKQAVMLGLAATISHTAVVWLIAFGGMVISKRFTAQSAEPWLQLISAVIIISTA
                     FWMFWRTWRGERNWLENMHEHDHEHHHHDHEDHHDHGHHHHHEHGEYQDAHARAHAND
                     IKRRFDGREVTNWQILLFGLTGGLIPCPAAITVLLICIQLKALTLGTTLVVSFSLGLA
                     LTLVTVGVGAAISVQQVAKRWSGFNTLAKRAPYFSSLLIGLVGVYMGVHGFMGIMR"
     gene            17262..17600
                     /gene="rcnB"
                     /locus_tag="NBI74_002742"
     CDS             17262..17600
                     /gene="rcnB"
                     /locus_tag="NBI74_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416610.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II) efflux transporter accessory
                     subunit RcnB"
                     /protein_id="HCD1933049.1"
                     /translation="MTIKNKMLLGALLLVTSAAWAAPATAGSTNTSGISKYELSSFIA
                     DFKHFKPGDTVPEMYRTDEYNIKQWQLRNLPAPDAGTHWTYMGGAYVLISDTDGKIIK
                     AYDGEIFYHR"
     gene            complement(17682..18716)
                     /locus_tag="NBI74_002743"
     CDS             complement(17682..18716)
                     /locus_tag="NBI74_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative fimbrial-like adhesin protein"
                     /protein_id="HCD1933050.1"
                     /translation="MEIRIMLFILMMMVMPVSYAACYSELSVQHNLVVQGDFALTQTQ
                     MATYEHNFNDSSCVSTNTITPMSPSDIIVGLYNDTIKLNLHFEWTNKNNITLSNNQTS
                     FTSGYSVTVTPAASNAKVNVSAGGGGSVMINGVATLSSASSSTRGSAAVQFLLCLLGG
                     KSWDACVNSYRNALAQNAGVYSFNLTLSYNPITTTCKPDDLLITLESIPVSQLPATGN
                     KTTINSKKGDIILRCKNLLGQQNQTSRKMQVYLSSSDLLTNSNTILKGAEDNGVGFIL
                     ESNGTPVTLLNITNSSKGYTNLKEIAAKSKLTDTTVSIPITASYYVYDTNKVKSGALE
                     ATALINVKYD"
     gene            complement(18732..21212)
                     /locus_tag="NBI74_002744"
     CDS             complement(18732..21212)
                     /locus_tag="NBI74_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HCD1933051.1"
                     /translation="MLRMTPLASAIVALLIGIEAYAAEETFDTHFMIGGMKDQQVANI
                     RLDDNQPLPGQYDIDIYVNKQWRGKYEIIVKDNPQETCLSREMIKRLGINTDNFASGK
                     QCLTFKQLIQGGSYTWDIGVFRLDFSVPQAWVEELEIGYVPPENWERGINAFYTSYYM
                     SQYYSDYKASGNSKSTYVRFNSGLNLLGWQLHSDASFSKTNNNPGVWKSNTLYLERGF
                     AQLLGTLRVGDMYTSSDIFDSVRFSGVRLFRDMQMLPNSKQNFTPRVQGIAQSNALVT
                     IEQNGFVVYQKEVPPGPFAITDLQLAGGGADLDVSVKEADGSVTTYLVPYAAVPNMLQ
                     PGVSKYDFAAGRSHIEGASKQSDFVQAGYQYGFNNLLTLYGGSMVANNYYAFTLGTGW
                     NTRFGAISVDATKSHSKQDNGDVFDGQSYQIAYNKFVSQTSTRFGLAAWRYSSRDYRT
                     FNDHVWANNKDNYRRDENDIYDIADYYQNDFGRKNSFSANMSQSLPEGWGSVSLSTLW
                     RDYWGRSGSSKDYQLSYSNNLRRISYTLAASHAYDENHHEEKRFNIFISIPFDWGDDV
                     TTPRRQIYMSNSTTFDDQGVASNNTGLSGTVGSRDQFNYGVNLSYQYQGNETTAGANL
                     TWNAPVATVNGSYSQSSAYRQAGASVSGGIVAWSGGVNLANRLSETFAVMNAPGIKDA
                     YVNGQKYRTTNRNGVVVYDGMTPYRENYLMLDVSQSDSEAELRGNRKIAAPYRGAVVL
                     VNFDTDQRKPWFIKALRADGQPLTFGYEVNDIHGHNIGVVGQGSQLFIRTNEVPPSVN
                     VAIDKQQGLSCTITFGKEIDESKNYICQ"
     gene            complement(21228..21902)
                     /locus_tag="NBI74_002745"
     CDS             complement(21228..21902)
                     /locus_tag="NBI74_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HCD1933052.1"
                     /translation="MKGLLALLISSMVLPAHAGIVIYGTRIIYPAEHKEVMVQLMNQG
                     NRSSLMQAWIDDGDTSLPPEKIQVPFMLTPPVAKIGANSGQQVKIKIMPNKLPTNKES
                     IFYLNVLDIPPNSPEQEGKNALKFAMQNRIKLFYRPAGIAPVNKATFKKLLVNRSGNG
                     LVIKNDSANWVTISDVKANNVKVNYETIMIAPLESQSVNVKSNNANNWHLTIIDDHGN
                     YISDKI"
     gene            complement(21983..22525)
                     /locus_tag="NBI74_002746"
     CDS             complement(21983..22525)
                     /locus_tag="NBI74_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCD1933053.1"
                     /translation="MKRSIIAAAVFSSFFMSAGVFAADVDTGTLTIKGNIAESPCKFE
                     AGGDSVSINMPTVPTTVFEGKAKYSTYDDAVGVTSSMLKISCPKEVAGVKLSLITNDK
                     ITGNDKAIASSNDTVGYYLYLGDNSDVLDVSAPFNIESYKIADGQYAIPFKAKYLKLT
                     DNSVNSGDVLSSLVMRVAQD"
BASE COUNT         5726 a         5735 c         5181 g         5956 t
ORIGIN      
        1 catgattatt ataattgaat aggattataa taaatgttct gtacaacatt tcctacataa
       61 gtaggaatta cagacataga ggcccttccg ggtaacccca tgggggactt taatatattt
      121 tgaatagtat attgaagatg ccactgttta gttgaatatt aggtatattc tcttttttaa
      181 aatttatcag tggctaccgt taatattcgc tgtccccatt gtaaggtcct ggtggtaagc
      241 actgaatccc catacctgaa ctgacttttt atcctccgac tttcatcctg ttctgactcc
      301 accttttctt ttctgctcta cactatctac agaccaatca taaaggcaca tacgatcatg
      361 gcagaatttc ccgccagctt actgattctt aatggcaaaa gtactgacaa tctacccttg
      421 cgcgaagcaa ttatgctgtt gcgtgaggaa ggaatgacga tccatgtgcg gatcacctgg
      481 gagaaaggcg atgctgcacg atttgtagag gaggcccgga agttaggcgt cgcaacagtg
      541 attgccggtg gtggcgatgg caccattaat gaagtttcta cggcattgat tcaatgtgag
      601 ggggatgaca tacccgcgct gggaatttta ccattaggaa ctgccaatga ttttgccact
      661 agtgtaggga ttcctgaggc actggataag gcgctgaaac tggcaattgc cggtaacgcc
      721 attgcgatag atatggcgca ggtcaacaaa caaacctgtt ttattaatat ggcgacaggc
      781 gggtttggga cgcgtattac cacagaaacg ccggaaaaat taaaagccgc gctgggtggc
      841 gtctcttaca tcattcatgg cttaatgcgc atggacactc tgcaaccgga ccgttgtgaa
      901 atccgcggtg aaaactttca ctggcaaggt gacgccctgg tcattggtat tggtaacggg
      961 cgtcaggccg gtggcggtca acaattgtgc ccgaacgcat taattaacga tggcttgctg
     1021 caactgcgca tttttaccgg cgatgaaatt attccgactc tcgtatcaac cttaaaatct
     1081 gacgaagata acccgaatat tatcgaaggt gcttcgtcgt ggtttgatat acaagcccca
     1141 cacgaaatca cttttaatct tgatggcgaa ccgttgagtg ggcaaaactt ccatattgaa
     1201 atacttccgg cggcgttgcg ttgtcgatta ccaccagatt gtccattgtt gcgttaatca
     1261 aactattctc tgttgaacca gagagaatcc gtaatgccgg atacggtgta aacaccttat
     1321 ccggcataca gaacaatact acaccgtaat taattttacc cgtgtcttct gcaatgccag
     1381 tttatccacc tcagacacag catcatcggt aataataatg tcaatttttt cgattggcag
     1441 tacctgatta aaaccgcgac gattaaattt cgatgaatca agaacggcaa tcactttatt
     1501 cgccgctgtc accatcgcac cgctaacgga ataaccttca ttaaaggtcg taataccatt
     1561 aaccgcatca ataccatcag caccgacaaa cattaaatcg gcattaatat cctgcaggga
     1621 acgttcagca atagatccat gcatcgagcg cgttttatgg cggaccgtgc caccgcagac
     1681 gaccagagta atgtctttat tttcggaaag ggcaaacgcc gccgggagac tgttggtaat
     1741 caccgtgata tttttggcgg tcattaatcc ttcggcgata agcattgtgg tacttccgct
     1801 gtcgagaata acggtcatcc cttcgtggat cattttgact gccgcctgcg ctattctgtt
     1861 tttcggcgcg ctggcgagct gaaatcgctc tttaaacccg acttcctggg tctcggtttc
     1921 gctattacct gacattattt tcgccgcacc gccatgaaag cgcgtaacca cgcctttttg
     1981 ttcgagaaag cgcaaatcgg cacggattgt cgcttccgag gcagcaaata ctcccgccag
     2041 atcctgaaca agcacggttc cctgttcatt cactaattgg atgatcttat tccttcgctc
     2101 gaatgaattc atcgtcattt acccatattc aattgtggct agtgtaaacg aaggggaacc
     2161 attggtgaat gatctgtttt gcacaatacc gggccagcat cacgctggcc cgcggtttca
     2221 gggaatgagc aacactttgc ccggcatagc attacgagcg atgtcacgca ccgcctgggc
     2281 aaagctttca aagcttccac ggtgagcgat taatggctcc aggcttaact tacgttctgt
     2341 cagcaaccgg ctcgccgttt cccactcctg ccccggccaa gggctggagt agttcatcca
     2401 actgccgata accgtcagct ctttacgcaa tattttgcca aacgttgcag atgttaaatg
     2461 cagatcctga tgcaacgtgc ccaccagcgc cagttgggca tgaggaccgg caatctctac
     2521 cgccagttcg acggtttgcg ggacgccagc cgtctcgagg ataagctgat taaagcgcag
     2581 ctcgcgtaaa acgccctgta tttgcggcgc gctcatttca cggctgttaa atgtttgcat
     2641 cgcaccgaaa gattttgcca gtgccagttt ttctgagcta atgtcgatag ccgtcacact
     2701 ctttgctccc agcgcgacag cgcactgaat tgccagcagg ccaatggttc cggcaccaat
     2761 aataataacg tttttattct cacatccttg cgctaaatgg aacgcatgca ggccaacggt
     2821 aatcggttca ataaaagcgc catcctcaat aggcatatcc gcaggtagtg caaagacatt
     2881 gtttcgctta acgacaatat attcggcaaa tccaccatca cgccgtgagc caataaaatc
     2941 atattttgcg cactgggaat aaaacccttt cagacactct ggacaagtaa aacagggtaa
     3001 taacggcaca caggcaaccg catcgccggg gtgtaaatca tcaacaccgg atcccaccgc
     3061 atcaatatag ccactaaatt catggcctaa cgttattgga taataatgtg caccattttt
     3121 aaatatcctg ggtaaatcgg aaccacataa gcccgagctg gcaattttta cccgcacctc
     3181 atcctgatgt ttaatttcag gaatgacgct ttctgctacg cgcacgatac catcagtatc
     3241 attcaccact gatttcataa aaactcctga ttgttaaggg ggataacctc cccctcaggt
     3301 aaaaattatt ctgcgagaac ggctttctct tgtttaataa aaccacgcgc tctacgccag
     3361 gtcatgaaaa taccggtaag ataaattgcg ccgataacaa tgaaaccggg aatattttgc
     3421 ggggagaaaa cctgaatcag taaccaggta atgggagaac cgccctgatc cattgaagcc
     3481 accatacccc cggcttttaa cgcgcccgca ttagccgcca gttgggtgtg caaaccaata
     3541 gtttgcgtcg cgatccacag ggtgatgctc ataatgatga cacccgagat taaggtgcgg
     3601 aacagatttc cacgatgcac cgcgaccgcc atcgccacaa agaagccgat ggtggcaagg
     3661 tcgccaaacg gcagcacctg attacccggc acacaaacag caattaaaat ggtgagtgga
     3721 ataaaaatca ggcttgccga taccaccgcc gtatgtccca gcagcaacgc cggatcaagg
     3781 ccaatcagga actcctgacc gccgaacttc gcctgtaaac gactacgggc ctgcttagcg
     3841 atgggcgtta aaccatccat gatgggttta atcacccgtg gcatcagcag cataactgcc
     3901 gccgttttta ccgccagctg caatacacct ttgacatcgt aacccgcgag gatgccgata
     3961 atcagcccca tcacaaaacc cacggtgacc ggttcgccaa aagggccaaa tttgcgctga
     4021 atatcatcgg cgctaaattt aattcggtta acgcctggga ttttctcgat gatagcatcg
     4081 accagcacag caattggccc catatacgcc gacgtaccgt gcggaatagc aatgccttcc
     4141 agctcaaaga aatcacgggt atcgcgggca aaccagtcgc cgagcttata aacaaacgcc
     4201 gcgtgaatta ccacacccgc catccctatc atccatgaac cggttgccag atgcagcaac
     4261 gcgccggtga aggtcatatg ccagatattc cagatatcaa catttaccac ccgcgtcata
     4321 cgggtcagta acatcgccac gttaaccaga atcgcaatcg gaatcgccac cagcgcaatt
     4381 tgcgacgccc aggtcatggg tgaagagccc ggccagccga catcgaccac atgcagattc
     4441 aggtcgaaat tttccgccat cgctttcgcc gccgggccaa tggaatccag cattaagcca
     4501 atcacaaggc caatgccaac aaagccaatc ccgatatgca gacccgcttt aaagcaatcg
     4561 cctgccttca tgcctaatat tttagaaaaa ataatgatga caatcggcag catcaccgta
     4621 gggccgaggt cgagaatata acgcatgact tctgaaaaca taggtcaccc ctgtaagata
     4681 gtcagaattt tattttgtaa tgcttcgata ccgacaccag aaacaaaagg catgccgtga
     4741 actaacggaa tatcgccaaa actgcgttca actttggcgg tagtacatat caaatgcacg
     4801 ccatccatat aggtttctat ttcattaacc cgacactgga ttaattcaac aggaatatta
     4861 tgactctgac acaactcttt aatttcttcc gccgccatcg tagaggtcgc taccgcgcct
     4921 ccgcaagcga caataatttt gcgtttcata aatagtcctt tttattgtta cagagggatt
     4981 tattcgtctg aatctaaaac atactttgtg aagtattcct ttaactgggg ttcaggaaga
     5041 gtgattagtg tctcgacgat atcgggctgt tgtaacttgc caaataaaca gcgtaaaagt
     5101 ttcaattgct gctgcggatt ttccacaatc aacgcaataa ccaacgatac cgccacgtcg
     5161 ttatcatcat ccgcttgctg aaaaagaact ttatttgttg gccttaacag ataaatggct
     5221 gacgacttag catgaatcgc ctcacaatgc ggtattgcaa tggcgtgctg ctcaagcatt
     5281 atcccggtag ggaattctgc ttctctggca attaatgcct gtggccaggt atcatgaacc
     5341 acacctttcg cgagcatctc attaccgata tgggttaaaa cttcgctacg atcgacaaaa
     5401 gaaattccgc cacgaacaaa caggttagtc atataccgtc cttattccgc acagccgtag
     5461 cgataggcgc gcaaaacatc ataaatttta tccataataa gctgatgcgg tattgctgat
     5521 aattccccgg actgaatgcg ttcaaattgt ttcggaagat actgactaat catgcccagt
     5581 gggatgtcta cgccttccag attcaccatc atcgtttcga cgctattttt aatccggcta
     5641 tgcggccagt aataacgaat acgatccgac aggctgtaac gaatatccag taatgaatcg
     5701 ttaaaacccg tgcgataata tttcttccag tactgcggtt cgtcgagcat cacttcttca
     5761 attaccgcca ggcaaccact gctattttcg ggggcgataa gttcctgctc aatttgcgcc
     5821 agcgcaaata tcgcctcgcg taacgcaaag gttaatgccg gaccgacttt caaaattgca
     5881 aaatgatcgc gaactaattc ccgataggcc gcctgagtct ggtaatcggt ggaatgcgct
     5941 tcataaacca ttttggtttt ttctatccat tgcgccaacg cctgagcttc ttgcgcctga
     6001 taatgaataa tattgctatg gtcaaattcc acaccaggct gcaccacgat ggcaatcacg
     6061 cgtgttaacg cctctgccag accgcgggca ataaacgctt tttgatgcgt gcgtaaagta
     6121 ttggcggcat cttcaacctg ggtgatgtgt actgactgaa tggcgctggc ctcaccgccc
     6181 ggaaccggaa cttcagtgcc aatgacatag ttcagttgct cacgctggca atcagtcgcc
     6241 acactttccg ccgccagaca aagcacagcg gcacgttcag caaccgtttc tggcgctaac
     6301 ggtatggaat cgtcagcgca ggacattgac gcatcaagat gaattttact gaagccggca
     6361 cgaacatatg cctttaccag ctcgacggat ttttccatcg ccgcatccgc attttcttgc
     6421 tgccagcagt ttggtcccag atggtcgccg ccgagaataa tacgctcacg agcaaatccg
     6481 actttatcgg caatagcaaa aacaaattcg cgaaagtctg ccggtgtcat tccggtataa
     6541 ccgccaaatt gattgacctg gtttgacgtt gcttcaatca gcactttacg cgtgctgttg
     6601 cgatcaaatg ccagcgccgc ttcgataacc aacggatgtg cagaacagac tgaacatatg
     6661 ccaatatgtt caccagcttt atgccgggca attaacgttt tcatgttttt tccttgttaa
     6721 atggcaggtg cgttatgccc tgccctcgca gccacaatcg gcaatcactt tgctcaccac
     6781 atcgcgcatt gcagatttag ccgactgcaa ataatcacgg ggatcggtcg cttcggggtg
     6841 ctcggtcagg taatttttta acgcctgcga gaaggcattt ttcagctccg ttgcaacgtt
     6901 gattttgcat atccccagtt tgatggtttg ctgaatatcc ttagtcgata atcctgacgc
     6961 gccatgcagc accagcggta aattcaccca ctggcgaatg ttctccagtc gagaaaaatc
     7021 aagcgccggt gctctggcat acataccgtg agccgtgccg atggcgaccg ccagggaatc
     7081 aatcccggtt gcctcggcaa attcacgcgc ctgaacgggg ttggtgtaca acgcatctgc
     7141 ttcattgact tgcacatcat cttcctggcc gccaagttgc cccagctccg cttcgacgct
     7201 gacatcaaag cgatggcaaa aatccaccac ctctttgacc cgtgaaatat tttgcgcaaa
     7261 aggcaaatgc gaggcgtcaa tcatgactga gcgcacgcca gaacgaacct tctgagcgat
     7321 atcgtcaaat tttgtgtgat ggtcgagatg aatcgccagt ggatggtgat attgcttcgc
     7381 catcgcgctg accagcgcca tcagattttc tgtaccggca tgagtaaatg tgccgggcgt
     7441 tccggcgatg atgaccggcg catgcatgct ggcagcggtt tccaccacca cttgcatcgt
     7501 ttcgagattg tgaatattga atgccggaac cgcataaccg ccgcgctgcg cgttgttcag
     7561 catctgcttt gtcgatacca cgtacatttt cacatcctgt catttgtttt cgatttcaaa
     7621 atataatgaa attatttgtt ttcaaatgtc gagataacga tcacaaaaac gacaatgtga
     7681 aaattaatca gaatgacagg caaaaaaaac ggcctcccgg gggaagccgt aacgaagtgc
     7741 gcgtgttttt atgccggatg caacgtaaac gccttttccg tcctacgggg tggttctgtt
     7801 cgtatgaggc gcatcaggcg atagtaattt tactatcgag ataaacgtcc tgtacggcgt
     7861 taatcagttt cacgccgtca gccatcgatt tcttgaacgc cttacgtcca agaatcagcc
     7921 ccattccgcc tgcgcgtttg ttgataaccg cagtacgcac tgcatcgctg aggtcagttt
     7981 caccgcctgc agcaccgccg gagtttatca acccggcccg gcccatatag cagttagcta
     8041 actgataacg caccaggtca atcgggtttt cactggtcag tttgctgtag actcgatcgt
     8101 cggtataacc gtaattaatc gctttatagc cgccgttatt ttccgccatt ttttgtttga
     8161 cgatatctgc acctatggtt gccgccagat ggttcgcctg accggtcagg tcggcggaaa
     8221 catggtaatc aacgccatct ttcttaaagg cggagttacg caaataggcc cacagcactg
     8281 tcaccatgcc cagctcgtgc gcacgttcaa aagccgcaga aatttcttca atctggcgac
     8341 gtgactcttc cgagccaaaa tagatagtcg caccaaccgc caccgcgccc atgttgaacg
     8401 cctgctccac gctggcatac agcgtttgat cgtaggtatt cgggtaactt agcgtctcgt
     8461 tgtgattaag tttgacgagg aatggaatgc gatgcgcgta acgccgcgat accgatgcca
     8521 gcacgccgta agttgacgcc acacagttac agcccgcttc gatcgccagt tcaacaatgt
     8581 ttttcgggtc aaagtagagc gggttagcgg caaatgaagc tccggcagag tgctcaacgc
     8641 cctggtcaac cggcagaata gaaagatatc ctgtgccagc cagacgcccg gtgttgtaca
     8701 acgtctgcat attacgtaac accgctggcg ggcgattatt gtcaatcatc acgcggtcta
     8761 cgtagtcatg tccggggaga taaagctggt cagaaggaat agtcatacaa cggtgctgta
     8821 aaaggttgtc ggcgtctttg ccaagcaact gcgcaatatc tgtcattact atgctcccgt
     8881 aaattccgat tggatatcgg ctatggattg tcctggcccg ccttttgcgg gcaatcataa
     8941 tcctggtcgt tacgggcgag tttttccatc ttttggatgt tttttcagcg ttttctgttg
     9001 gctcgattca tcagaaaaaa tgttaccgcg gtcaagtttt caccgcaaag gtatttaaaa
     9061 ggtattacta agtggtattg tcatcgcgta ccttacatta cctgtcatga aggaattaaa
     9121 agatgaaaac aacagcaaag ctgtcgttca tgatgtttgt tgaatggttt atctggggcg
     9181 cgtggtttgt gccattgtgg ttgtggttaa gtaaaagcgg ttttagtgcc ggagaaattg
     9241 gctggtcgta tgcctgtacc gccattgcgg cgatcctgtc gccaattctg gttggctcca
     9301 tcactgaccg ctttttctcg gcgcagaaag tgctggcggt attgatgttc gctggcgcgg
     9361 tactgatgta tttcgctgcg caacagacca cttttgccgg gttcttcccg ttactgctgg
     9421 cctactcgct aacctatatg ccgaccattg cgctgactaa cagtatcgct tttgccaacg
     9481 tgccggatgt ggagcgtgat ttcccacgca ttcgtgtgat gggcactatc ggctggattg
     9541 cctctggtct ggcatgtgga ttcttgccgc aaatgctggg ttatgccgat atctcaccga
     9601 ctaacatccc gctactgatt accgccggaa gttctgctct gctcggtgtg tttgcgtttt
     9661 tcctgcccga cacgccgcca aaaagcaccg gcaaaatgga tattaaagtc atgctcggcc
     9721 tggatgcgct gatcctgctg cgcgataaaa acttcctcgt ctttttcttc tgttcattcc
     9781 tgtttgcgat gccactggcg ttctattaca tctttgccaa cggttatctg accgaagttg
     9841 gcatgaaaaa cgccactggc tggatgacgc tcggccagtt ctctgaaatc ttctttatgc
     9901 tggcattgcc gtttttcact aaacgctttg gtatcaaaaa ggtattgttg cttggtctgg
     9961 tcaccgctgc gatccgctat ggcttcttta tttacggtag tgcggatgaa tatttcacct
    10021 acgcgttact gttcctcggt attttgctgc acggcgtaag ttacgatttt tactacgtta
    10081 ccgcttacat ctatgtcgat aaaaaagctc ccgtgcatat gcgtaccgcc gcgcaggggc
    10141 tgatcacgct ctgctgccag ggcttcggca gtttgctcgg ctatcgtctt ggcggtgtga
    10201 tgatggaaaa gatgttcgct tttcaggaac cggtaaacgg actgactttc aactgggccg
    10261 ggatgtggac tttcggcgcg gtgatgattg ccattatcgc cgtgctgttc atgatttttt
    10321 tccgcgaatc cgacaacgaa attacggcta tcaaggtcga tgatcgcgat attgcgttga
    10381 cacaagggga agttaaatga aaacagaacg tattctcggt gctctttatg ggcaggcgtt
    10441 aggggatgcg atggggatgc cctccgagct ttggccgcgc agtcgcgtca aagcacactt
    10501 tggctggatt gaccgttttc tgcctgggcc aaaggagaat aacgcggcct gttattttaa
    10561 ccgcgccgaa ttcaccgacg atacctcgat ggcgctgtgt ctggcggatg cgttactgga
    10621 acgtgaaggc aagatcgatc cggatctgat tgggcgtaat attctcggct gggcgctgcg
    10681 tttcgacgcc tttaacaaaa acgtactagg tccgacctcg aagattgcgc ttaacgccat
    10741 tcgcgacggt aaacccgttg ctgaactgga aaataacggc gtgaccaacg gcgcagcgat
    10801 gcgcgtctcg ccattaggtt gtttgcttcc ggcgcgcgat gttgattcct ttattgatga
    10861 tgtggcgctg gcctccagcc cgacccataa atccgatctg gcggttgcgg gggcggtagt
    10921 catcgcatgg gcgatttctc gtgccattga cggagaaagc tggtcagcga ttgttgattc
    10981 actgccttca attgcgcgac atgctcaaca aaaacgcatc actaccttca gcgcctcact
    11041 ggcagcacgt ctggagattg cgctgaaaat tgtgcgcaat gccgatggca ccgaatccgc
    11101 cagcgaacag ctttaccagg tcgttggcgc aggtaccagt actattgagt ccgttccgtg
    11161 cgccattgcg ctggttgaac tggcacaaac cgacccgaac cgttgcgccg tcctgtgcgc
    11221 taaccttggc ggcgacacag acaccatcgg tgctatggcg acggcaattt gcggcgcgtt
    11281 gcatggcgtt aacgctatcg atcctgcgtt aaaggcggaa ctggatgcag taaatcagct
    11341 tgatttcaac cgctatgcca cagcgctggc gaaatatcgt caacaacggg aggcgatatg
    11401 agcggcgctc gattacacac gctgctgcct gaattaacca cgcgtcagcc agtgatggtc
    11461 gtcggcgcgg cggtcattga tgtgatcgcc gacgcttacg ctctgccctg gcgtgggtgc
    11521 gatatcgaac tgaaacagca gagcgttaac gttggcggct gcgcactgaa tattgccgtg
    11581 gcgttaaagc gcctcggcat cgaagcgggt aatgccctgc cgctcggtca gggcgtgtgg
    11641 gcggagatta ttcgcaaccg gatggcgaaa gaggggttaa tcagcctgat cgataacgct
    11701 gaaggcgata acggctggtg tctggcgctg gttgagccgg atggcgaacg cacttttatg
    11761 tcattcagtg gtgtggaaaa tcagtggaat cgcgaatggc tggcgcgatt aaccgttgcg
    11821 cctggcagcc tgctctattt ttccggttat caactggcct cgccctgcgg cgaattgtta
    11881 gtggaatggc tggaagagct gcaagacgtg acgccattta tcgattttgg cccacgtatt
    11941 ggcgatatcc cggatgcatt actggcgcgg atcatggcct gtcgaccttt agtgtcactc
    12001 aatcgtcaag aggctgagat tgccgccgaa cgttttgctt tatccgcaga gataacaaca
    12061 cttggcaagc aatggcagga gaaatttgcc gcgccgttga tcgttcgtct cgataaagag
    12121 ggcgcatggt atttcagcaa cgacgcttct ggctgcattc cggcatttcc aacgcaagtt
    12181 gtagacacca ttggggcggg cgacagtcat gccggcggcg tacttgccgg gctggcctct
    12241 ggtctgccac tggcggatgc cgtattactg ggcaatgcag tggcgtcgtg ggttgtcggg
    12301 catcggggcg gtgattgtgc gccaacgcgc gaggaactac tcctcgcaca caaaaacgta
    12361 tagatcgctg cgacagtggc tgatgctgta ctcaataggc cgttgctgtt ggtcaagcgc
    12421 cacttgcttg atcaccagca ccggtatttt gctatctaac tgaatatgtg actgaaactc
    12481 ggcatccggc atccgggcgc taacgcggga acgcgtacgt tgtgggtaaa tgtgctggct
    12541 gcggaagtag tcataaagag aaatcccgat ggcatcaaca tcatgaatta aatgtgccgg
    12601 aacccacgat tcctcaatcg ataccgcctc ttcatcgaca tagcgaatac gcttaagcaa
    12661 aaaaacatca cttcctgcct cgaccgccag ctgctgcgcg acgtcttcgg gacatttcac
    12721 gacacgcttg ttaacccata gcgtatcggg ctttttcccg cgcaacacca cctgctgaga
    12781 aaaacctcgc gcttctttca acgagtattc gaagatgttg ttgatctgcg tgccgtaacc
    12841 acgcgaacgc gtcaccacgc cctcttcttc cagcgactgc atcgccttgc gcaccgtaat
    12901 gcgcgaaacg ccggttaact gacttaaatc acgctcaccg ggcaaaatat tgccatgctc
    12961 cagcaccccg ctgcgcacgg cattttttac cgtttcggca aacttaatat aaagcggcgt
    13021 gttatccgcc gctaaaatac gctcgttcag ttgggcaatc agctgggtat gcgcttgttc
    13081 catttgtctt tttcctgccg gggaatctgg tgggagtata tcactgcttc ttatttaagg
    13141 cgtttcttta atcccatcaa cgaatccctg caactcctcc tctgtgccat taaacacatt
    13201 aaaatccacc ggaccattaa tgccatcgac ctgtccacgg tcggaatgct gccagaagcg
    13261 ccaggccatg ccgtcattgt ccgggcgacg ttgataatag tgagccaccc accacggata
    13321 ttcattgaaa tagcccgcca gattggtgtg ataaaaaacg gctcctgagt aaataatcgg
    13381 cttttttccc gtacgttttt cgaccatttt tagccactga cttacccgct tgcgtaattc
    13441 ttttgccgat aattttcctc gctcttctac gtccagtacc gcagggaaat cgccttgtga
    13501 gaaatccacc gtttgcagaa ataatcgcgc ctgaactgaa gcggatactg acggggagaa
    13561 ataatgatac gccccgcgca gcaggccatt ttcccggctt agttgccagt tacgcgaaaa
    13621 ataggggtcc accagctttt cgccttccgt cgccttaata aaagcaaact gtaagcggat
    13681 accgttgtcg cgcatttttg ccacccgctg ccagtcgatc cgctcctgcc agcgcgaaac
    13741 atctattccg tgaatggtgt aactggcagg aatgcgaata gcgaaagatt taaccgggcg
    13801 ataaccgtag aagtggatgt agtctttaat ctgaattgct gtcgaataga aaaaattcac
    13861 cgtctgacgc ggataaattg caacgatgga aattagccca agagcgcata ataaaacggt
    13921 gaattttttt cgactggtga ttctcaactg cattggtttc atccctgaat gtcagcacca
    13981 aaggctgaca ataaccaaag cagctatagt acggtgctca ccaccaggcg tggaagtggt
    14041 gaaccggacc aataccgtga ccaacttcca gcgtgtcggc ctgggctaat gccgatgaaa
    14101 gccagctttt tgcctcctgt acggtgtcag cccagtttgt atggcgcggg cgcagtgcag
    14161 ccaacgccgc agagagcgta caaccagtgc cgtgagtgtt tttggtcata atgcgctgtg
    14221 cggtaaaccg ttgttcaccc tcgcgggtaa acagccagtc cgggctttgc tcatcatcca
    14281 gatgaccacc tttcattagc actgcgccac agcccatcgc caacagcgat cgcccttgtt
    14341 ccagcatttc ctgttcggtg cgcgcgtgtg gcgcgtcgag caaggcggct gcttcgggca
    14401 agtttggcgt tattaatgaa acctgtggca ataatcgact gcgtagcgtg gcaactgccg
    14461 aaggtgaaag cagcgggtcg ccgctttttg ccagcataac ggtgtcgagt accacgtttt
    14521 ggatctgata acgttgcaac cgttctgcca ccgcttcaac aatatcggtt tccgccaaca
    14581 taccgatttt agtggtgtcg attcgcacat cgctgaacac cgaatcgagc tgggcggcga
    14641 caaaatcagg ctcaatgcga tacaccgact gtacaccacg ggtattttgc gccaccagtg
    14701 cagtaataac tgagcaacca taagcgccaa gtgccgagaa ggttttaaga tcggcctgaa
    14761 tccccgcacc accactcgga tcagtaccgg caatcgtcag agcgttaatt cgtttcatgc
    14821 ctgcacctcc tgcgtcaatt gccagagcgc atcaaggaaa tgtggaacaa aactgcctgg
    14881 cccctcgctt ctggcgactg cgcgttctcc ggcttgtttc atccagtgac aggcagatgc
    14941 gatattttcc agcgtatcgc ctggtagcgc acaacaggca gcgacaaccg ccgatagtgc
    15001 acagccagtt cctaccactt tggtcattaa cggatcgcca ccgtgaatgc caatgatacg
    15061 atgtccatcg gtaacataat ccacctcgcc agtgaccacg acgattgcgc cagtttcccg
    15121 tgccagtgtt tgtgcagcag gtatcgcgtt agctgcggca tcagtggtat ccactccccg
    15181 tccaccatta gcaacgccag ctgatgccat gatttccgaa gcattaccac gtatggcggt
    15241 tggcttatgg gacagaagtt ccagacaaaa acggcggcga taatcgagcg caccaaccgc
    15301 aaccggatca agcgtccagg gcgtttgtga tcgtgttgct tgctcaacgg cagcactcat
    15361 cgcttgcgct cgtggccgcg tcagcgtgcc aacgttaatc aacaaggcac tggcgatagc
    15421 cgcaaactga ctggcctctt cggtttcgat aaccatcgct ggcgatgcgc cgagcgccag
    15481 caaggtattg gcagtaaaag tttgcaccac atcgttggtc atgcagtgca caagagggga
    15541 atgttggtga aaaaggtgta acgcgtgcgc agattgcgct gaactcagca ggtcgacttg
    15601 catagtttgc tcctgccata acgtgaagaa gcaatgacct ggtggtccgt gacttcccta
    15661 cgctggcatt atccagatca ggtgatacgg gtatttctca gccttcacaa agaagggcac
    15721 cccgagtcgt ttggttgcga taataaatcg cagaggagga tggtaatgtc cagcgcacgc
    15781 gttgtaaacg gggaaaaacg gcggtattac aataacgttg tgaaattatt tgatataaga
    15841 atccagcacc ttcagaacga catccagatc ttcttcacgt tttagctcat ccccctggtg
    15901 aacgatgtgt tccgtcagat ggcctttaat cacttcccgc atcagaccgt ttaccgcgcc
    15961 acggatagca gcaatctgtt gtaaaactgc agcgcattca tgcggttcgt cgagcatttt
    16021 cttgagcgcc acgacctggc cctgaatctt actggcacgc gctttcagtt tctgtttatc
    16081 acggattgta tgagacatgg caacacctgg ttaacaagaa tatgaaaaat catagcacta
    16141 ttaatctact ggggggtagt atcaggtact gggggggagt agaatcagat tgccgaatta
    16201 atactaagaa ttattatcat gaccgaattt acaacacttc ttcagcaagg aaacgcctgg
    16261 ttcttcatcc ccagcgccat cttacttggt gcgcttcatg gtctggaacc ggggcactca
    16321 aaaacgatga tggcggcgtt tatcatcgcc atcaaaggca ccattaaaca ggcggtgatg
    16381 ctcggactgg cagcaactat ttcgcatacc gcagtggtct ggttaattgc ctttggcggg
    16441 atggtgatca gcaaacgctt tactgctcaa tcagcagaac cgtggctcca gttgatttcc
    16501 gcagtgatca ttattagcac cgcgttctgg atgttctggc gtacctggcg cggcgaacgc
    16561 aactggctgg agaatatgca cgagcatgat catgagcatc atcaccacga tcacgaagat
    16621 caccacgacc atggacatca tcaccatcac gaacatggcg agtatcagga tgcccatgca
    16681 cgagcccatg ccaatgacat taaacgacgc tttgatggta gagaggtcac caactggcaa
    16741 attttgctat ttggcttaac cggtggcctt atcccctgcc cggcagcaat taccgtgctg
    16801 ttgatttgca ttcagttgaa agccctgaca ctgggcacaa cactggtcgt cagtttcagc
    16861 ctcggcctgg cgttaacgct tgtcaccgta ggcgttggcg cagcaatcag cgttcagcag
    16921 gtcgcaaaac gctggagcgg atttaacact ctcgctaaac gcgctcccta tttttccagt
    16981 ctgttgattg gcttagttgg tgtgtatatg ggcgtacatg gcttcatggg cataatgcga
    17041 taactcaatc ctttaggctt gcatgttatc ttcagcccag gatgattctt aaatcagcta
    17101 tttccgctga ctctctgctc gctaaggatg cttttagggc atcctttcat ttacactttt
    17161 tacgaaatca tgggatcact aacaaaatat cgcttgtcag ttatattgta tggcaggaaa
    17221 gatatgcgac tgatattaca gatccccaaa gtggagagtt tatgaccatt aaaaataaga
    17281 tgttgctggg tgcgcttttg ctggttacca gtgccgcctg ggccgcacca gccaccgcgg
    17341 gttcgaccaa tacctcggga atttctaagt atgagttaag cagtttcatt gctgacttta
    17401 agcatttcaa accaggtgac accgtcccag agatgtaccg caccgatgag tacaacatta
    17461 agcagtggca gttacgtaac ctgcccgcgc ctgatgccgg gacgcactgg acctatatgg
    17521 gtggcgcgta cgtgttgatc agcgacaccg acggtaaaat cattaaagcc tacgacggtg
    17581 agatttttta tcatcgctaa aaaaaagccc cctcggagag aggggggaat gcagacacct
    17641 taatattttt attattagcc acttgctcgt cttgcttgtt attagtcgta tttcacgttg
    17701 attaatgcgg ttgcctccag tgcgccagat ttaactttgt ttgtatcgta gacgtagtaa
    17761 ctggccgtta tcggaattga aaccgttgta tctgtaagtt ttgactttgc cgcaatttct
    17821 tttaaatttg tatatccttt actgctgtta gtgatattta aaagtgtgac tggcgtacca
    17881 ttactttcaa gaataaatcc tacgccatta tcttccgcac ctttcaggat cgtgttgctg
    17941 ttggttaaca agtcagaact tgataaatac acctgcattt tccgtgatgt ttgattttgt
    18001 tgacctaata aatttttaca acgcagaata atatcccctt ttttactatt tattgttgtt
    18061 ttgttacctg tggctggtaa ttgtgaaacg ggaatactct ctaacgtaat taataaatcg
    18121 tccggtttgc aggttgtggt tatcgggttg tatgacaatg tcagattaaa ggaatagaca
    18181 cctgcatttt gtgccaatgc atttctgtag ctatttacac atgcatccca tgacttacca
    18241 cctaataaac acagtagaaa ttgtactgcg gcactcccac gtgtcgatga tgaagcactg
    18301 gataatgtcg caactccatt aatcatcact gaaccaccgc cccccgcaga aacattcact
    18361 tttgcattac tggccgcagg tgtcaccgtt accgaataac cactggtgaa actggtctga
    18421 ttatttgaca atgtaatgtt gtttttattt gtccattcaa aatgtaaatt taatttaatg
    18481 gtatcgttat aaagtccaac aataatatcc gacgggctca taggggtgat agtatttgta
    18541 ctcacgcatg acgaatcatt aaaattatgc tcatatgtcg ccatttgtgt ttgagtaagt
    18601 gcaaaatccc cctgaacaac caaattgtgc tgaacagata actcactata acatgccgca
    18661 tagctcacag gcataaccat catcattaat ataaatagca ttatgcgaat ttccatagaa
    18721 ccacctgact attactggca aatataattt ttactttcat caatctcttt accgaaggtg
    18781 attgtgcatg aaagcccttg ttgcttatca attgccacat taaccgatgg cggtacttca
    18841 ttggtgcgaa taaataactg gcttccctgg ccgacaacac caatattatg accatggata
    18901 tcattgactt cataaccaaa cgttaatggt tgcccatccg ctcttaatgc ttttataaac
    18961 catggcttgc gctgatcggt atcaaaatta accagtacaa ccgcgccgcg ataaggggcg
    19021 gcaattttcc ggttgccacg taattctgct tcgctatcgc tttgtgacac atccaacatc
    19081 aggtaatttt cccgataagg tgtcattccg tcgtatacca ccactccatt acggtttgtt
    19141 gtgcgatatt tttgcccatt gacataagca tctttaattc ctggcgcatt catcacagca
    19201 aacgtttcag aaagacggtt tgccagatta acgccacccg accaggcgac aatgccccct
    19261 gaaacactgg ctccagcctg tcgataagca ctcgactgac tataactgcc attcactgtc
    19321 gcaaccggcg cgttccaggt taaattcgcc ccagctgtcg tttcatttcc ctgatactga
    19381 taactcaggt tgaccccata gttaaactga tcccggcttc caacggttcc tgataatccc
    19441 gtattatttg aggcaacccc ctgatcatca aacgtcgttg agttagacat atatatttgc
    19501 cgacgaggcg tcgtaacgtc atcaccccaa tcaaagggaa tcgatataaa aatattaaaa
    19561 cgtttctctt catgatgatt ctcgtcataa gcatggcttg ccgcgagggt atagcttatc
    19621 cttcgcaggt tgttggaata actcaactga taatccttac tgctgccgct acgcccccag
    19681 taatctcgcc ataacgtact taaggacaca gaaccccagc cttctggcaa tgactgactc
    19741 atattggcag aaaagctatt tttacggcca aaatcgttct ggtaataatc ggcaatgtca
    19801 tagatatcgt tttcatcacg gcgataatta tctttattgt ttgcccatac gtgatcatta
    19861 aatgtccgat aatcacgcga cgaataacgc caggccgcca gaccaaaacg tgtcgacgtt
    19921 tggctcacaa atttgttgta ggcaatttga taactttgcc cgtcaaacac atcgccgttg
    19981 tcttgtttac tatgcgactt tgtggcatcg acggaaatgg caccaaagcg tgtattccag
    20041 ccagtcccca aagtgaacgc gtaataatta ttcgcgacca tcgagccacc atacagcgtt
    20101 aataagttat taaaaccata ctgataaccc gcctggacaa aatcactttg tttgctcgcc
    20161 ccttcaatat ggctacgacc cgccgcaaaa tcatatttcg acacgccggg ttgcagcata
    20221 tttggtaccg ctgcataagg caccagatag gtggttaccg aaccgtccgc ctctttcacg
    20281 ctaacatcaa gatccgctcc accaccggcc aactgcaaat ctgtaatcgc aaacgggcct
    20341 ggaggaacct ctttctgata aaccacaaaa ccattctgtt caatagttac cagcgcgtta
    20401 ctctgagcaa tcccctgcac tcgtggcgta aaattttgtt tcgagttagg caacatctgc
    20461 atatcacgaa acaaccgcac accgctgaag cgaacagaat caaaaatatc gcttgatgtg
    20521 tacatatcac ccacacgaag cgtgccgaga agttgggcaa atccacgttc cagatacagg
    20581 gtattgctct tccacacccc tggattgtta tttgttttac tgaaactggc atcagaatgc
    20641 agttgccacc ccaataaatt taacccgctg ttaaaacgta catatgtact cttgctatta
    20701 ccggacgctt tataatcgct gtaatactga ctcatataat aagaggtata aaacgcatta
    20761 ataccccgct cccagttttc aggtggtaca tagccaattt ccagttcttc cacccaggct
    20821 tgcgggacac tgaaatcgag acgaaaaact ccgatatccc aggtatagct cccgccctga
    20881 ataagttgct taaatgttaa acattgctta ccgctggcaa agttatcggt attaatgcct
    20941 aaccgcttga tcatttccct tgataaacat gtttcttgcg ggttgtcttt aacaatgatc
    21001 tcatatttcc cacgccattg cttattgaca taaatatcga tgtcatactg ccccggcaag
    21061 ggttgattat catcaagacg aatatttgca acctgctggt ctttcattcc acctatcatg
    21121 aaatgggtat caaaggtttc ttcagctgca taagcttcaa tgccgatcaa taacgctacg
    21181 attgctgatg caagtggggt cattctcaac ataggcagct cctgcaatta aattttgtca
    21241 ctaatatagt tgccatggtc atcgataatg gtcagatgcc agttatttgc attattactt
    21301 ttgacattaa cactctgact ttctaagggg gcaatcataa tagtttcata attgactttg
    21361 acattattag ctttgacatc cgaaatcgtc acccaattag ctgagtcatt ttttatcacc
    21421 aatccattgc cactgcgatt taccagtaac tttttaaagg tcgctttatt taccggagca
    21481 atacccgctg gccggtaaaa caacttaatt ctgttttgca tcgcaaactt cagtgcattc
    21541 ttaccttctt gttctggact atttggtgga atatccagaa cattcagata aaaaatactc
    21601 tctttattag tgggtagttt attcggcata attttaattt ttacttgctg cccggaattt
    21661 gcccctattt ttgccactgg tggcgttaac atgaaaggaa cctgaatttt ttctggtggt
    21721 aatgacgtat cgccatcatc aatccacgcc tgcatcagcg aagaacgatt tccctggttc
    21781 atcagctgca ccatcacttc tttgtgctct gccgggtaaa taatacgcgt cccgtagata
    21841 acaataccgg catgtgcagg aaggaccata gaagaaatga gtaaagctaa taatcctttc
    21901 atcttaatgc ctcttaaatt aaaaggccgc catggggata gcggccatgt taattacctg
    21961 aagaatcttg caattttatt tattaatcct gcgccacacg cataaccagg gaagataaca
    22021 catcacctga attcactgag ttatctgtca gtttcaggta ttttgcttta aacggaatgg
    22081 catattgacc gtccgctatt ttataactct cgatgttgaa aggtgcagaa acatccagga
    22141 catcgctgtt atcacctaaa tagagataat aacccacggt atcgttgcta ctggctatcg
    22201 ccttatcgtt tccggttatt ttatcgttgg taatcaacga gagttttaca ccagcaactt
    22261 ctttcggaca gctaattttt aacatgctgc tggttacacc gactgcatca tcataggtcg
    22321 agtatttagc tttaccttca aagacagtgg ttggtacagt tggcatatta atacttactg
    22381 aatcgccacc cgcttcgaat ttacacggag attctgcaat attcccttta atggttaatg
    22441 ttccggtatc aacgtctgcg gcaaataccc cagcgctcat aaaaaaagaa gaaaaaacag
    22501 cggcagcaat aattgaacgt ttcataatat gctccttaat aaaatctatt tttggttgtt
    22561 aagagtctgt atctctttga aacaaatttc tcacacca
//
LOCUS       DAHHTG010000071        22480 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.071, whole genome shotgun sequence.
ACCESSION   DAHHTG010000071 DAHHTG010000000
VERSION     DAHHTG010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22480)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22480)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22480
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.071"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            complement(353..1264)
                     /gene="gsiD"
                     /locus_tag="NBI74_002747"
     CDS             complement(353..1264)
                     /gene="gsiD"
                     /locus_tag="NBI74_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiD"
                     /protein_id="HCD1933054.1"
                     /translation="MRLFNWRRQAVLNAMPLVKPDQVRTPWHEFWRRFRRQHMAMTAA
                     LFVILLIVVAIFARWIAPYDAENYFDYDNLNNGPSLQHWFGVDSLGRDIFSRVLVGAQ
                     ISLAAGVFAVFIGAAIGTLLGLLAGYYEGWWDRLIMRICDVLFAFPGILLAIAVVAVL
                     GSGIANVIIAVAIFSIPAFARLVRGNTLVLKQQTFIESARSIGASDMTILLRHILPGT
                     VSSIVVFFTMRIGTSIISAASLSFLGLGAQPPTPEWGAMLNEARADMVIAPHVAVFPA
                     LAIFLTVLAFNLLGDGLRDALDPKIKG"
     gene            complement(1267..2187)
                     /gene="gsiC"
                     /locus_tag="NBI74_002748"
     CDS             complement(1267..2187)
                     /gene="gsiC"
                     /locus_tag="NBI74_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiC"
                     /protein_id="HCD1933055.1"
                     /translation="MLNYVIKRLLGLIPTLFIVSVLVFLFVHMLPGDPARLIAGPEAD
                     AQVIELVRQQLGLDQPLYHQFWHYISNAVQGDFGLSMVSRRPVADEIASRFMPTLWLT
                     ITSMVWAVIFGMAAGIIAAVWRNRWPDRLSMTIAVSGISFPAFALGMLLIQVFSVELG
                     WLPTVGADSWQHYILPSLTLGAAVAAVMARFTRASFVDVLSEDYMRTARAKGVSETWV
                     VLKHGLRNAMIPVVTMMGLQFGFLLGGSIVVEKVFNWPGLGRLLVDSVEMRDYPVIQA
                     EILLFSLEFILINLVVDVLYAAINPAIRYK"
     gene            complement(2205..3743)
                     /gene="gsiB"
                     /locus_tag="NBI74_002749"
     CDS             complement(2205..3743)
                     /gene="gsiB"
                     /locus_tag="NBI74_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter substrate-binding
                     protein GsiB"
                     /protein_id="HCD1933056.1"
                     /translation="MARAVHRSGLVALGIATALMASCAFAAKEVVVAVGSNFTTLDPY
                     DANDTLSQAVAKSFYQGLFGLDKEMKLKNVLAESYTVSDDGLTYTVKLREGIKFQDGT
                     DFNAAAVKANLDRASDPANHLKRYNLYKNIAKTEAIDPTTVKITLKQPFSAFINILAH
                     PATAMISPAALEKYGKEIGFHPVGTGPYELDTWNQTDFVKVKKFAGYWQPGLPKLDSI
                     TWRPVADNNTRAAMLQTGEAQFAFPIPYEQAALLEKNKNIELMASPSIMQRYISMNVT
                     QKPFDNPKVREALNYAINRPALVKVAFAGYATPATGVVPPSIAYAQSYKPWPYDPAKA
                     RELLKEAGYPNGFSTTLWSSHNHSTAQKVLQFTQQQLAQVGIKAQVTAMDAGQRAAEV
                     EGKGQKESGVRMFYTGWSASTGEADWALSPLFASQNWPPTLFNTAFYSNKQVDDFLAQ
                     ALKTNDPAEKTRLYKAAQDIIWQESPWIPLVVEKLVSAHSKNLTGFWIMPDTGFSFED
                     ADLQ"
     gene            complement(3763..5634)
                     /gene="gsiA"
                     /locus_tag="NBI74_002750"
     CDS             complement(3763..5634)
                     /gene="gsiA"
                     /locus_tag="NBI74_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter ATP-binding protein
                     GsiA"
                     /protein_id="HCD1933057.1"
                     /translation="MPHSDELDAGDVLAVENLNIAFMQEQQKIAAVRNLSFSLQRGET
                     LAIVGESGSGKSVTALALMRLLEQAGGLVQCDKMLLQRRSREVIELSEQSAAQMRHVR
                     GADMAMIFQEPMTSLNPVFTVGEQIAESIRLHQNASREEAMVEAKRMLDQVRIPEAQT
                     ILSRYPHQLSGGMRQRVMIAMALSCRPAVLIADEPTTALDVTIQAQILQLIKVLQKEM
                     SMGVIFITHDMGVVAEIADRVLVMYQGEAVETGSVEQIFHAPQHPYTRALLAAVPQLG
                     AMKGLDYPRRFPLISLEHPAKQESSIEQKTVVDGEPVLRVRNLVTRFPLRSGLLNRVT
                     REVHAVEKVSFDLWPGETLSLVGESGSGKSTTGRALLRLVESQGGEIIFNGQRIDTLS
                     PGKLQALRRDIQFIFQDPYASLDPRQTIGDSILEPLRVHGLLPGKEAAARVAWLLERV
                     GLLPEHAWRYPHEFSGGQRQRICIARALALNPKVIIADEAVSALDVSIRGQIINLLLD
                     LQRDFGIAYLFISHDMAVVERISHRVAVMYLGQIVEIGPRRAVFENPQHPYTRKLLAA
                     VPVAEPSRQRPQRVLLSDDLPSNIHLRGEEVAAVSLQCVGPGHYVAQPQSEYAFMRR"
     gene            complement(5621..6586)
                     /gene="iaaA"
                     /locus_tag="NBI74_002751"
     CDS             complement(5621..6586)
                     /gene="iaaA"
                     /locus_tag="NBI74_002751"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HCD1933058.1"
                     /translation="MGKAVIAIHGGAGAISRAQMSLQQELRYIEALSAIVETGQKMLV
                     AGESALDVVTEAVRLLEECPLFNAGIGAVFTRDETHELDACVMDGNTLKAGAVAGVSH
                     LRNPVLAARLVMEQSPHVMMIGEGAENFAFAHGMERVSPEIFSTPLRYEQLMAAREEG
                     ATVLDHSGAPLDEKQKMGTVGAVALDLDGNLAAATSTGGMTNKLPGRVGDSPLVGAGC
                     YANNASVAVSCTGTGEVFIRALAAYDIAALMDYGGLSLAEACERVVMEKLPALGGSGG
                     LIAIDHEGNVALPFNTEGMYRAWGYAGDTPTTGIYREKGDTVATQ"
     gene            6790..8025
                     /gene="moeA"
                     /locus_tag="NBI74_002752"
     CDS             6790..8025
                     /gene="moeA"
                     /locus_tag="NBI74_002752"
                     /EC_number="2.10.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HCD1933059.1"
                     /translation="MEFITGLMSLDTALNEMLSRVTPLTAQETLPLVQCFGRILASDV
                     VSPLDVPGFDNSAMDGYAVRLADIASGQPLPVAGKSFAGQPYHGEWPAGTCIRIMTGA
                     PVPEGCEAVVMQEQTEQTDNGVRFTAEARSGQNIRRRGEDISAGAVVFPAGTRLTTAE
                     LPVIASLGIAEVPVIRKVRVALFSTGDELQLPGQPLGDGQIYDTNRLAVHLMLEQLGC
                     EVINLGIIRDDPHALRAAFIEADSQADVVISSGGVSVGEADYTKTILEELGEIAFWKL
                     AIKPGKPFAFGKLSNSWFCGLPGNPVSATLTFYQLVQPLLAKLSGNTASGLPARQRVR
                     TASPLKKSPGRLDFQRGVLQRNADGELEVTTTGHQGSHIFSSFSLGNCFIVLERDRGN
                     VEVGEWVEVEPFNALFGGL"
     gene            8025..8774
                     /gene="moeB"
                     /locus_tag="NBI74_002753"
     CDS             8025..8774
                     /gene="moeB"
                     /locus_tag="NBI74_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HCD1933060.1"
                     /translation="MAELSDQEMLRYNRQIILRGFDFDGQEALKDSRVLVVGLGGLGC
                     AASQYLASAGVGNLTLLDFDTVSLSNLQRQTLHSDATVGQPKVESARDALTRINPHIA
                     ITPVNALLDDAELAAMIAKHDLVLDCTDNVAVRNQLNAGCFAAKVPLVSGAAIRMEGQ
                     ITVFTYQDGEPCYRCLSRLFGENALTCVEAGVMAPLIGVIGSLQAMEAIKLLAGYGKP
                     ASGKIVMYDAMTCQFREMKLMRNPGCEVCGQ"
     gene            complement(8850..9512)
                     /gene="fsa"
                     /locus_tag="NBI74_002754"
     CDS             complement(8850..9512)
                     /gene="fsa"
                     /locus_tag="NBI74_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415346.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HCD1933061.1"
                     /translation="MELYLDTSDVVAVKALSRIFPLAGVTTNPSIIAAGKKPLEVVLP
                     QLHEAMGGQGRLFAQVMATTAEGMVNDARKLRSIIADIVVKVPVTAEGLAAIKMLKAE
                     GIPTLGTAVYGAAQGLLSALAGAEYVAPYVNRIDAQGGSGIQTVTDLHQLLKMHAPQA
                     KVLAASFKTPRQALDCLLAGCESITLPLDVAQQMISYPAVDAAVAKFEQDWQGAFGRT
                     SI"
     gene            9643..10542
                     /locus_tag="NBI74_002755"
     CDS             9643..10542
                     /locus_tag="NBI74_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HCD1933062.1"
                     /translation="MIFNIQRYSTHDGPGIRTVVFLKGCSLGCHWCQNPESRARTQDL
                     LYDPRLCLEGCELCTKTAPEVIELALNGLLIHREKLTPEHFTALTDCCPTQALTVCGE
                     VKSVEEIMATVLRDKPFYDRSGGGLTLSGGEPFMQPEMATALLQASHEAGIHTAVETC
                     LHVPWKYIAPSLSYIDLFLADLKHVADAPFKQWTDGNAARVLDNLKKLAAAGKKIIIR
                     VPLIQGFNADETSVKAITDFAADELHVSEIHFLPYHTLGINKYHLLNLPYDAPEKPLD
                     APELLDFAQQYACQKGLTATLRG"
     gene            10548..12980
                     /locus_tag="NBI74_002756"
     CDS             10548..12980
                     /locus_tag="NBI74_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HCD1933063.1"
                     /translation="MTTLKLDTLSDRIKAHKNALVHIVKPPVCTERAQHYTEMYQQHL
                     DKPIPVRRALALAHHLANRTIWIKHDELIIGNQASKVRAAPIFPEYTVSWIEKEIDDL
                     ADRPGAGFAVSEENKRVLHEVCPWWRGQTVQDRCYGMFTDEQKGLLATGIIKAEGNMT
                     SGDAHLAVNFPLLLEKGLDGLREKVAERRSRINLTVLEDLHGEQFLKAIDIVLVAVSE
                     HIERFAALAREMAATETRESRRDELLTIAENCDLIAHQPPQTFWQALQLCYFIQLILQ
                     IESNGHSVSFGRMDQYLYPYYRRDVELNQTLDREHAIEMLHSCWLKLLEVNKIRSGSH
                     SKASAGSPLYQNVTIGGQNLVDGQPMDAVNPLSYAILESCGRLRSTQPNLSVRYHAGM
                     SNDFLDACVQVIRCGFGMPAFNNDEIVIPEFIKLGIEPQDAYDYAAIGCIETAVGGKW
                     GYRCTGMSFINFARVMLAALEGGRDATSGKVFLPQEKALSAGNFNNFDEVMDAWDTQI
                     RYYTRKSIEIEYVVDTMLEENVHDILCSALVDDCIERAKSIKQGGAKYDWVSGLQVGI
                     ANLGNSLAAVKKLVFEQGAIGQQQLAAALADDFDGLTHEQLRQRLINGAPKYGNDDDT
                     VDTLLARAYQTYIDELKQYHNPRYGRGPVGGNYYAGTSSISANVPFGAQTMATPDGRK
                     AHTPLAEGASPASGTDHLGPTAVIGSVGKLPTAAILGGVLLNQKLNPATLENESDKQK
                     LMILLRTFFEVHKGWHIQYNIVSRETLLEAKKHPDQYRDLVVRVAGYSAFFTALSPDA
                     QDDIIARTEHML"
     gene            13126..13941
                     /gene="ybiV"
                     /locus_tag="NBI74_002757"
     CDS             13126..13941
                     /gene="ybiV"
                     /locus_tag="NBI74_002757"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000114249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase YbiV"
                     /protein_id="HCD1933064.1"
                     /translation="MSVKVIVTDMDGTFLNDAKTYNQPRFMAQYQELKKRGIEFVVAS
                     GNQYYQLISFFPELKDEISFVAENGALVYEHGKQLFHGELTRHESRIVIGELLKDKQL
                     NFVACGLQSAYVSENAPEAFVALMAKHYHRLKPVKDYQEIDDVLFKFSLNLPDEQIPL
                     VIDKLHIALDGIMKPVTSGFGFIDLIIPGLHKANGISRLLKRWDLSPQNVVAIGDSGN
                     DAEMLKMARYSFAMSNAAENIKQIARYATDDNNHEGALNVIQAVLENKPPFNL"
     gene            complement(14141..15733)
                     /locus_tag="NBI74_002758"
     CDS             complement(14141..15733)
                     /locus_tag="NBI74_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATPase"
                     /protein_id="HCD1933065.1"
                     /translation="MLVSSNVTMQFGSKPLFENISVKFGGGNRYGLIGANGSGKSTFM
                     KILGGDLEPTLGNVSLDPNERIGKLRQDQFAFEEFTVLDTVIMGHKELWEVKQERDRI
                     YALPEMSEEDGYKVADLEVKYGEMDGYSAEARAGELLLGVGIPVEQHYGPMSEVAPGW
                     KLRVLLAQALFADPDILLLDEPTNNLDIDTIRWLEQVLNERDSTMIIISHDRHFLNMV
                     CTHMADLDYGELRVYPGNYDEYMTAATQARERLLADNAKKKAQIAELQSFVSRFSANA
                     SKSRQATSRARQIDKIKLEEVKASSRQNPFIRFEQDKKLFRNALEVEGLTKGFDNGPL
                     FKNLNLLLEVGEKLAVLGTNGVGKSTLLKTLVGDLQPDSGTVKWSENARIGYYAQDHE
                     YEFENDLTVFEWMSQWKQEGDDEQAVRSILGRLLFSQDDIKKPAKVLSGGEKGRMLFG
                     KLMMQKPNILIMDEPTNHLDMESIESLNMALELYQGTLIFVSHDREFVSSLATRILEI
                     TPERVIDFSGNYEDYLRSKGIE"
     gene            15952..16872
                     /gene="ldtB"
                     /locus_tag="NBI74_002759"
     CDS             15952..16872
                     /gene="ldtB"
                     /locus_tag="NBI74_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HCD1933066.1"
                     /translation="MNMKLKTLFAAAFAVVGFCSTASAVTYPLPTDGSRLVGQNQVIT
                     IPEGNTQPLEYFAAEYQMGLSNMMEANPGVDTFLPKGGTVLNIPQQLILPDTVHEGIV
                     INSAEMRLYYYPKGTNTVIVLPIGIGQLGKDTPINWTTKVERKKAGPTWTPTAKMHAE
                     YRAAGEPLPAVVPAGPDNPMGLYALYIGRLYAIHGTNANFGIGLRVSHGCVRLRNEDI
                     KFLFEKVPVGTRVQFIDEPVKATTEPDGSRYIEVHNPLSTTEAQFEGQEIVPITLTKS
                     VQTVTGQPDVDQVVLDEAIKNRSGMPVRLN"
     gene            complement(16930..18048)
                     /locus_tag="NBI74_002760"
     CDS             complement(16930..18048)
                     /locus_tag="NBI74_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000056422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion transporter"
                     /protein_id="HCD1933067.1"
                     /translation="MSLPFLRTLQGDRFFQLLILVGIGLSFFVSFAPKSWPAAIDWHT
                     IITLSGLMLLTKGVELSGYFDVLGRKMVRRFATERRLAMFMVLAAALLSTFLTNDVAL
                     FIVVPLTITLKRLCEIPVNRLIIFEALAVNAGSLLTPIGNPQNILIWGRSGLSFAGFI
                     AQMAPLAGAMMLTLLLLCWCCFPGKALQYHTGGQTPEWKPRLVWSCLGLYIVFLTALE
                     LKQELWGLVIVAAGFALLARHVVLSVDWTLLLVFMAMFIDVHLLTQLPVLQGVLSNVS
                     HLSEPGLWLTAIGLSQVISNVPSTILLLNYVPPSLLLAWAVNVGGFGLLPGSLANLIA
                     LRMANDRRIWWRFHLYSIPMLLWAALVGYVLLVMIPAG"
     gene            complement(18045..18512)
                     /gene="mntR"
                     /locus_tag="NBI74_002761"
     CDS             complement(18045..18512)
                     /gene="mntR"
                     /locus_tag="NBI74_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-binding transcriptional regulator
                     MntR"
                     /protein_id="HCD1933068.1"
                     /translation="MSRRAGTPTAKKVTQLVNVEEHVEGFRQVREAHRRELIDDYVEL
                     ISDLIREVGEARQVDMAARLGVSQPTVAKMLKRLATMGLIEMIPWRGVFLTAEGEKLA
                     QESRERHQIVENFLLVLGVSPEIARRDAEGMEHHVSEETLDAFRLFTQKHGAK"
     gene            18698..18826
                     /gene="mntS"
                     /locus_tag="NBI74_002762"
     CDS             18698..18826
                     /gene="mntS"
                     /locus_tag="NBI74_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganase accumulation protein MntS"
                     /protein_id="HCD1933069.1"
                     /translation="MNEFKRCMRVFSHSPFKVRLMLLSMLCDMVNNKPQQDKPSDK"
     gene            19098..20681
                     /gene="opgE"
                     /locus_tag="NBI74_002763"
     CDS             19098..20681
                     /gene="opgE"
                     /locus_tag="NBI74_002763"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HCD1933070.1"
                     /translation="MNLTLKESLVTRSRVFSPWTAFYFLQSLLINLGLGYPFSLLYTA
                     AFTAILLLLWRTLPRVQKVLVGISSLVAACYFPFAQAYGAPNFNTLLALHSTNMEEST
                     EILTIFPWYSYLVGIFIFALGVIAIRRKKESEKARWNTFDSLCLVFSVATFFVAPVQN
                     LAWGGVFKLKDTGYPVFRFAKDVIVNNNEVIEEQERMAKLSGMKDTWTVTAVKPKYQT
                     YVVVIGESARRDALGAFGGHWDNTPFASSVNGLIFADYIAASGSTQKSLGLTLNRVVD
                     GKPQFQDNFVTLANRAGFQTWWFSNQGQIGEYDTAIASIAKRADEVYFLKEGNFEADK
                     NTKDEALLDMTAQVLAQEHSQPQLIVLHLMGSHPQACDRTQGKYETFVQSKETSCYLY
                     TMTQTDDLLRKLYDQLRNSGSSFSLVYFSDHGLAFKERGKDVQYLAHDDKYQQNFQVP
                     FMVISSDDKAHRVIKARRSANDFLGFFSQWTGIKAKEINIKYPFISEKKAGPIYITNF
                     QLQKVDYNHLGTDIFDPKP"
     gene            complement(20730..21245)
                     /gene="ompX"
                     /locus_tag="NBI74_002764"
     CDS             complement(20730..21245)
                     /gene="ompX"
                     /locus_tag="NBI74_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpX"
                     /protein_id="HCD1933071.1"
                     /translation="MKKIACLSALAAVLAFTAGTSVAATSTVTGGYAQSDAQGQMNKM
                     GGFNLKYRYEEDNSPLGVIGSFTYTEKSRTASSGDYNKNQYYGITAGPAYRINDWASI
                     YGVVGVGYGKFQTTEYPTYKHDTSDYGFSYGAGLQFNPMENVALDFSYEQSRIRSVDV
                     GTWIAGVGYRF"
     gene            complement(21298..21363)
                     /locus_tag="NBI74_002765"
     CDS             complement(21298..21363)
                     /locus_tag="NBI74_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1933072.1"
                     /translation="METFCYMKWPVRHHKSRRVSH"
     gene            21598..>22480
                     /gene="rhtA"
                     /locus_tag="NBI74_002766"
     CDS             21598..>22480
                     /gene="rhtA"
                     /locus_tag="NBI74_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/homoserine exporter RhtA"
                     /protein_id="HCD1933073.1"
                     /translation="MPGSLRKMPVWLPIVILLVAMASIQGGASLAKSLFPLVGAPGVT
                     ALRLALGTLILIAFFKPWRLRFAKEQRLPLLFYGVSLGGMNYLFYLSIQTVPLGIAVA
                     LEFTGPLAVALFSSRRPVDFVWVVLAVLGLWFLLPLGQDVSHVDLTGCALALGAGACW
                     AIYILSGQRAGAEHGPATVAIGSLIAALIFVPIGALQAGEALWHWSVIPLGLAVAILS
                     TALPYSLEMIALTRLPTRTFGTLMSMEPALAAVSGMIFLGETLTPIQLLALGAIIAAS
                     MGSTLTVRKESKIKELDI"
BASE COUNT         5529 a         6147 c         5525 g         5279 t
ORIGIN      
        1 ttaaagaaat agccaacgtt tttctgaata agttcaaagc ggctatcggc acgttttacc
       61 gcgcgttccg ataccagttg atagttaatg atgaaaaaaa gaccaataaa tgacaatgct
      121 gccaataaaa ataaaattat cagccttatc tttatctttt cgtataaact cagcatgtta
      181 atacatttaa aaaaataact gtctgaacgc tgtgccgctg caaaatagtc ccttgctgcg
      241 gctttttaac caaattaccc gattctacaa aatcagcaga aagttacaac acttgcttag
      301 ggcaataatt tcctgtgagg tgattaccct ttcaagcaat attcaaacgt aactatcctt
      361 taattttcgg atccagcgca tcgcgtaaac catcgcccaa caaattgaac gccagtacgg
      421 tcagaaaaat agccagggcc ggaaatacag cgacatgcgg cgcgataacc atatccgctc
      481 gtgcctcatt gagcattgca ccccactctg gtgtcggcgg ctgcgcaccg aggccgagaa
      541 atgacaggct ggcggcagag ataatcgagg tgccaatgcg catggtgaaa aacaccacga
      601 tagaagagac ggtcccaggc aagatatgac gcaacaaaat ggtcatatcg ctggcaccaa
      661 tactgcgtgc tgactcgata aaggtttgct gtttcaacac cagcgtgttg ccgcgcacca
      721 gccgggcaaa cgcggggatg gaaaaaatgg cgactgcgat aatcacgtta gcaatgccgc
      781 ttcccaacac cgcaacaaca gcgatcgcca gtaaaatacc cgggaaggca aacagcacat
      841 cgcaaatgcg catgatcagc cgatcccacc agccttcata atatccagcc agcaagccca
      901 gcaacgtccc gatcgccgca ccgataaaca cggcaaacac gcccgccgcc agcgagattt
      961 gcgcaccaac caggacacgg ctgaaaatgt cgcgccccag tgaatcgaca ccaaaccagt
     1021 gctgcaaaga aggtccgtta ttcagattgt cataatcaaa ataattttcg gcgtcatagg
     1081 gagcgatcca gcgtgcaaaa atggccacca caatcaataa aataacgaac aatgcggcgg
     1141 tcatcgccat atgctgacgg cgaaatcgtc gccagaattc atgccacggt gtacgtacct
     1201 ggtcaggttt gaccagtggc atggcgttta acaccgcctg acgtcgccag ttaaatagtc
     1261 gcatccttac ttgtacctga tagccgggtt aatggcggcg taaagcacat ccaccactaa
     1321 gttgataaga ataaattcca gcgagaaaag cagaatttcc gcctgaatca ctgggtaatc
     1381 gcgcatttct acggagtcaa cgagtaagcg tccaagtccg ggccagttga aaactttctc
     1441 caccacgatg gaaccaccga gcaagaagcc aaactgtaag cccatcatgg tcactaccgg
     1501 gatcatcgcg ttgcgtagcc cgtgtttgag gacaacccag gtttcgctca cccctttcgc
     1561 cctcgcggta cgcatataat cttcgcttaa aacgtcgaca aacgacgcgc gggtaaagcg
     1621 cgccatcacg gcggccactg ccgcgccgag cgtcagggag ggtaaaatgt agtgctgcca
     1681 actgtctgct cccacggtag gcagccagcc cagttcaacg gagaatacct gaattaaaag
     1741 catccccaga gcaaatgccg gaaacgagat ccccgacacc gcaatggtca tactcaatcg
     1801 atccggccaa cggttacgcc agacggcggc gataattccc gccgccatac caaatatcac
     1861 cgcccagacc atactggtta tggtcagcca gagcgttggc ataaagcggc tggcaatctc
     1921 atcggcaacc ggacgacgcg acaccatcga caggccaaaa tccccctgca cagcattgct
     1981 gatatagtgc cagaactggt gatacagcgg ctgatccaac cccaactgct gacgcaccag
     2041 ttctataacc tgcgcatcag cttcgggccc ggcaatcaat cgcgccggat cgccgggcag
     2101 catatggaca aataaaaaca ccagcaccga gacgataaac agcgtcggaa tcaaccccag
     2161 taagcgtttg ataacgtaat taagcattcc actccctgcg ttgcttattg caaatccgcg
     2221 tcttcaaagc tgaagccggt gtctggcatg atccaaaaac cggtcaggtt tttactgtgt
     2281 gccgacacca gtttttctac caccagcggg atccacggcg actcttgcca gatgatatcc
     2341 tgcgccgcct tatacaagcg ggtcttttcc gccggatcat tagttttcag tgcctgagcc
     2401 aggaagtcat ccacctgttt attgctgtaa aacgcggtat taaacagcgt tggtggccag
     2461 ttctgcgagg caaacagcgg cgatagagcc cagtcggctt cgccggttga agccgaccag
     2521 ccagtgtaga acatccgcac gccgctctct ttttgccctt taccttcaac ttctgccgcc
     2581 cgttgtccgg catccatcgc agtcacctgg gctttaatcc cgacctgcgc taactgctgc
     2641 tgggtaaatt gcagcacttt ctgcgcagtg ctgtggttat gtgacgacca cagcgtggta
     2701 ctgaaaccgt tgggatatcc cgcctctttc agtaattcgc gcgctttcgc cggatcgtaa
     2761 ggccacggtt tataactttg cgcgtaggcg atactcggcg gtaccacacc agtagctggc
     2821 gttgcatagc ccgcgaaggc cactttcacc agcgccgggc ggttaatggc gtaattcagc
     2881 gcctcacgga ccttcgggtt atcgaacggc ttttgcgtca cgttcatact gatataacgc
     2941 tgcataattg acggactggc catcaactcg atatttttgt ttttctccag cagtgcggcc
     3001 tgctcgtagg gaatggggaa agcaaactgc gcttcaccgg tttgcagcat tgccgcgcgg
     3061 gtgttgttat ccgccaccgg acgccaggtt atgctgtcca gtttgggcaa tcctggctgc
     3121 cagtaacccg cgaatttttt caccttcaca aaatcggtct gattccaggt atccagttca
     3181 tacggcccgg ttcccaccgg atgaaaacca atctccttgc catatttttc cagcgctgcc
     3241 ggtgatatca tcgcggtcgc cggatgggca agaatattaa taaacgctga gaatggctgt
     3301 ttgagggtaa tctttaccgt tgtcggatcg atcgcttccg ttttggcgat attcttatac
     3361 aggttatagc gtttaagatg attcgccgga tcgctggccc gatccagatt cgctttcacc
     3421 gccgctgcgt tgaaatcggt gccatcctgg aatttaatgc cttcccgcaa tttcacggtg
     3481 taagtaaggc cgtcatcgga aacggtgtaa ctctccgcca gcacgttttt cagtttcatc
     3541 tctttatcca gaccgaacag cccctggtaa aacgatttcg ctacggcctg agataacgtg
     3601 tcatttgcgt catacggatc gagcgtggtg aagttcgatc ccaccgccac caccacctct
     3661 ttggcagcga atgcacaaga tgccatcaac gctgtcgcaa tgcccagcgc cactaaccca
     3721 ctacggtgta cagctcttgc cattttattc tccgcctgaa tgttatctac gcatgaatgc
     3781 gtattctgat tgtggttgtg cgacgtaatg ccccggcccg acgcattgca acgagacggc
     3841 tgcaacctct tcgccacgca gatgaatatt gctgggaaga tcgtccgaca gcagtacacg
     3901 ctgcggtcgt tgtcgggacg gttcagcgac cggaactgcc gccagtaatt tacgcgtata
     3961 aggatgctgc ggattttcaa agaccgcgcg ccgtggacca atttcaacaa tttgcccgag
     4021 atacatcacc gccacacgat gactaatccg ctcaaccacg gccatatcgt gggagataaa
     4081 cagatacgca atgccgaaat cacgctggag atcgagcaac aagttgataa tctgcccgcg
     4141 aatagaaaca tccagcgccg agacggcttc gtcggcaatg atcacttttg gattcaatgc
     4201 caacgcgcga gcaatgcaga tgcgctggcg ctgaccgccg gaaaactcat gagggtaacg
     4261 ccaggcatgt tccggtaaca gacccacgcg ctccagcaac cacgcaacgc gtgcagccgc
     4321 ttctttacct ggcaataaac cgtgtacgcg cagcggttcg agaatcgaat caccgatggt
     4381 ctgacgtggg tccagcgaag cgtaagggtc ctgaaaaata aactgaatat cgcggcgcaa
     4441 tgcctgaagt ttaccgggtg acaaggtatc gattcgctga ccgttaaaga taatttcgcc
     4501 gccctgcgat tcgaccaggc gcagcaacgc ccgcccggtg gtggatttac cgctgccaga
     4561 ctcgcccacc agcgataacg tttcgccagg ccagagatca aaactgactt tctcaacggc
     4621 atgcacttcc cgcgttacgc gattcaacaa accgctgcgc aaagggaaac gggtgaccag
     4681 attacgtacc cgtaaaacag gttcgccatc caccaccgtt ttctgctcga tggatgactc
     4741 ctgtttcgct ggatgttcaa gcgatatcaa tgggaaacgt cggggataat ctaacccttt
     4801 catcgcacca agttgcggaa cagcagctaa cagcgcacgg gtataaggat gttgcggtgc
     4861 atgaaaaatc tgttcgacgc tacccgtttc caccgcctcg ccctgataca tcaccagtac
     4921 ccgatcggca atctctgcca ccacgcccat atcgtgagtg ataaagataa cgcccatcga
     4981 catctctttt tgcaatactt tgattaattg caggatctgc gcctgaatag tgacatccag
     5041 cgcggtggtt ggctcatcgg caatcagcac cgccgggcgg catgacagcg ccatcgcaat
     5101 catcactcgc tggcgcatcc cgccagagag ttgatgcgga taacgtgaaa gaatggtttg
     5161 tgcctccgga atgcgtacct gatccagcat ccgcttcgcc tcgaccatcg cttcttcacg
     5221 actggcgttc tgatgcagac gaatagattc agcaatctgt tcacccacag taaataccgg
     5281 gttcagcgat gtcatcggct cctgaaatat catcgccata tccgccccgc gcacatggcg
     5341 catttgtgca gcactctgct cgctaagttc aattacttcg cgactgcgcc gctgcaacag
     5401 cattttatcg cactgcacta aaccgcccgc ctgttccaac aggcgcatca atgccagcgc
     5461 agtcactgac ttaccggagc cggattcgcc aacaattgcc agcgtctcac cgcgttgcag
     5521 gctaaaagag agattgcgga ccgcagctat tttctgctgc tcctgcataa aggcaatatt
     5581 cagattttca accgccagca catcaccggc atcaagttca tcactgtgtg gcaacggtgt
     5641 cccctttttc acggtagata ccggtggttg gcgtatcgcc tgcgtagccc caggcgcgat
     5701 acattccttc ggtgttaaac ggtagcgcga cattcccttc atggtcgata gcgattaagc
     5761 caccgctacc gccaagcgca gggagttttt ccattactac ccgctcgcag gcttccgcga
     5821 gacttaatcc gccgtaatcc attaacgcgg cgatatcata tgccgccagc gcgcggatga
     5881 agacttcgcc cgtaccggta caggaaaccg ccacactggc gttattggca tagcatccgg
     5941 cccccactaa gggactatcg ccaactcgtc cgggtaattt attggtcatt ccgcccgtgg
     6001 acgtggctgc cgccagattg ccgtctaaat ccaacgccac ggcccctacg gtgcccattt
     6061 tctgtttttc atccagtggc gcaccgctat ggtcgaggac tgttgcccct tcctcgcgcg
     6121 ctgccattag ttgttcataa cgcaaaggtg tggagaaaat ctccggcgag acgcgctcca
     6181 tgccatgagc aaacgcaaaa ttttctgccc cttcgccaat catcatcaca tgcgggcttt
     6241 gctccatcac cagccgggcg gcaagaaccg gattacgcag atgactaacg cccgccaccg
     6301 caccggcttt cagggtgtta ccatccatca cacaggcgtc cagttcatgg gtttcatcac
     6361 gcgtaaagac agcgccaatt ccggcgttaa acagtggaca ctcttccagc agacgcaccg
     6421 cttccgtcac cacatccagc gcactttcgc ccgctaccag cattttctgc ccggtttcaa
     6481 caatggcaga caacgcctcg atgtagcgta attcctgttg cagactcatc tgcgcgcggc
     6541 taattgcacc tgcgccacca tgaattgcaa tgactgcttt gcccatagta ttcgtccatt
     6601 aagagtggct cgccactttt gctataaata tcagaatttt tatgaatgac tcattcaatt
     6661 tttgaatata gaaagatgga tatggcatgt aaaggcagga gccagcgcta cgcccgaggt
     6721 ggcatacagt ctgtggcgtt tttctgacat aataggcaaa ttcgattttg cctccgcagg
     6781 agtgttttca tggaatttat caccggattg atgtcgctcg acaccgcgct taatgagatg
     6841 ctttctcgcg ttaccccact gaccgcccag gaaacgctgc cactggttca gtgttttggc
     6901 cgtattctgg cgagcgatgt cgtttcgcca ctggatgtcc cggggtttga taactccgct
     6961 atggacggct acgcggtgcg tttagccgat attgcctccg ggcaaccgct gcccgttgct
     7021 ggtaaatcct ttgccggtca gccgtaccat ggtgaatggc ctgcgggtac ctgcattcgt
     7081 attatgaccg gtgcgccggt gccggaaggc tgcgaagcgg tggtgatgca ggagcagact
     7141 gaacaaacgg acaatggcgt gcgttttact gctgaagcgc gtagcgggca aaatattcgc
     7201 cgtcgcggtg aagatatctc tgcaggtgcg gttgttttcc cggcggggac tcgcctgact
     7261 accgctgaac tgccagtgat tgcttcgcta gggattgccg aagttccggt gattcgtaaa
     7321 gtgcgtgtag cgcttttttc taccggtgat gaactccagt tgcccggtca gccgctgggc
     7381 gacggccaaa tctacgatac caaccgtctc gccgtacacc tgatgttaga acagttggga
     7441 tgcgaggtaa tcaacttagg gattatccgc gacgatcccc atgccctgcg cgccgcattt
     7501 attgaagccg acagccaggc ggatgtggtg atcagttccg gcggtgtttc agtgggtgaa
     7561 gcggattaca ccaaaacgat tcttgaagag ctgggggaga tcgccttctg gaaactggcg
     7621 attaaaccgg gtaaaccgtt cgcgttcggt aaactttcta atagttggtt ctgcggcctg
     7681 ccgggcaacc cggtttcagc gacgctgacc ttctatcaac tggtacagcc tttgctggca
     7741 aaactaagcg gcaataccgc cagcggcctg cccgcgcgcc agcgtgtacg cacggcatcg
     7801 cctctgaaga aatcgccagg acgccttgat ttccagcgcg gcgtgctgca acgcaacgcc
     7861 gatggcgaac tggaagtgac gaccaccgga catcagggtt cacatatatt tagctccttt
     7921 agcctcggca actgctttat cgtgctggaa cgcgatcgcg gcaatgtgga agtgggcgaa
     7981 tgggtggaag tagaaccgtt taacgcgttg ttcggaggcc tgtaatggcg gaactcagcg
     8041 atcaggagat gctgcgctac aaccggcaaa tcattctgcg cggttttgat tttgacggtc
     8101 aggaagcatt gaaagattcc cgcgtgctgg tcgtcggcct tggtggactc ggctgcgcag
     8161 cttcgcagta tctggcaagc gccggtgtcg gcaacctgac gctgctcgac ttcgacacgg
     8221 tttcgctctc caatctgcaa cgccagacgc tgcacagtga tgccacggtc gggcaaccaa
     8281 aggtggaatc cgcccgtgac gccctgacgc ggatcaaccc acatatcgcg attacgccag
     8341 tcaatgcgct gcttgatgac gctgaacttg ctgcgatgat tgcaaaacac gatctggtgc
     8401 tcgactgtac ggataacgtt gcagtacgta atcaactgaa cgcaggctgt tttgccgcga
     8461 aggtgccgct ggtttccggc gcggcaattc gtatggaagg gcaaatcacg gtcttcactt
     8521 atcaggacgg cgaaccgtgc tatcgctgcc ttagccgttt gtttggcgag aacgcgttaa
     8581 cctgcgtaga agcaggcgtg atggcaccgt taatcggcgt aattggttcg ttgcaggcaa
     8641 tggaagcgat caaactgctg gcaggttatg gcaaaccagc cagcgggaaa atcgtgatgt
     8701 acgatgcgat gacctgtcag tttcgcgaaa tgaaactgat gcgcaatccg gggtgtgagg
     8761 tgtgcgggca gtaactgcca cctccaggcc agataagacg cggcactccg ccacgtcatc
     8821 agcaatttat gaggatgcag aacgtccggt taaatcgacg ttctgccaaa cgctccctgc
     8881 cagtcctgct caaacttcgc cacagcggca tcaaccgccg gatagctaat catctgttgt
     8941 gccacatcca gtggcagagt aattgattca caccctgcca gtaagcagtc cagcgcctga
     9001 cgcggggttt tgaaactcgc ggctaacact ttcgcctgcg gcgcatgcat tttcaataac
     9061 tggtgtaagt cggtcacagt ctgaataccg ctaccgccct gagcatcaat acgattaacg
     9121 taaggcgcaa catattccgc acctgccagc gccgacagta gcccttgtgc tgcgccatat
     9181 accgcggttc ccagcgtcgg aatcccttcc gctttcaaca tcttaatagc tgccagcccc
     9241 tcagcggtca ccggaacttt caccacgata tccgcaataa tagaacgcaa tttacgtgcg
     9301 tcattcacca tcccttcagc agtggtagcc attacctggg caaacagacg cccctgaccg
     9361 cccatcgctt cgtgaagttg cggaagcaca acctccagcg gttttttacc cgctgcgata
     9421 atgcttgggt tagtggttac acccgccagc ggaaaaatac gtgacagcgc cttcaccgca
     9481 acaacgtctg aagtatccag atacagttcc ataaccatcc tcaaaatgta ctttttatct
     9541 gaatgtagac tatcacggga aaactctctg aacagttgac gtacatcaat ccaactttca
     9601 ttcgaaagca ttttaatgtt tgaacgaaac agagagacaa ctatgatttt caatattcaa
     9661 cgctactcaa cccatgatgg ccccggtatc cgcacggtcg tatttcttaa aggctgttcg
     9721 ctgggctgcc actggtgtca gaacccggaa agccgcgccc gcacgcagga tctgctgtat
     9781 gacccacgac tctgtctgga aggctgtgag ctgtgcacta agaccgcacc ggaagtgatt
     9841 gagctcgcgt tgaatggttt gcttattcat cgggaaaagt taaccccgga gcattttacg
     9901 gcgctaaccg actgctgccc aacacaggcg ttaaccgtgt gtggtgaagt gaaaagcgtt
     9961 gaggagatca tggcaaccgt tttgcgcgat aaaccgtttt acgatcgcag cggcggcggc
    10021 ttaacacttt ctggcggaga accctttatg cagccggaaa tggcgacagc gctactgcaa
    10081 gccagccacg aagccggtat tcatactgcg gtagaaacct gtctgcatgt gccgtggaaa
    10141 tatatcgccc cttctctgtc ctatattgat ctgtttcttg ccgatttaaa acacgttgcc
    10201 gacgcgccgt ttaaacagtg gaccgacggt aacgccgcca gggtgctgga taatctgaaa
    10261 aaactcgccg cagcgggcaa aaaaatcatt atccgcgtgc cactgattca aggctttaat
    10321 gccgacgaaa cttctgtgaa agccattacc gattttgccg ccgacgagct gcacgttagc
    10381 gaaattcatt tcctgcccta ccacacgctg ggcatcaaca aatatcactt acttaatctg
    10441 ccctatgacg ccccggaaaa accgcttgat gcgccagaac tgctcgactt tgcccagcag
    10501 tatgcctgcc agaaagggtt aaccgcgacc ttacgaggat aacaaccatg accacactga
    10561 aactggacac gctcagcgat cgcattaaag cgcacaaaaa tgcgctggtg catattgtga
    10621 aaccgccggt ctgtaccgag cgcgcgcagc actataccga gatgtatcaa caacatctcg
    10681 ataagccgat cccggtacgt cgcgcgctgg cactggcgca tcacctggcg aatcgcacca
    10741 tctggatcaa gcacgatgag ttgatcattg gcaaccaggc aagcaaagtt cgcgccgcgc
    10801 cgatcttccc ggaatatacc gtctcctgga tcgaaaaaga gattgatgat ctggcagatc
    10861 gtcccggtgc tggttttgcg gtgagcgaag agaacaaacg cgttctgcat gaagtgtgcc
    10921 cgtggtggcg cggtcagacc gtacaggatc gctgctacgg catgtttacc gatgagcaaa
    10981 aaggtctgct ggcgaccggc atcattaaag cggagggcaa tatgacctcc ggcgatgcgc
    11041 acctggcggt caatttcccg ctactgctgg aaaaaggtct tgatggtctg cgtgagaaag
    11101 tggcggaacg tcgctcgcgc atcaatctga cggttctgga agatctgcac ggcgagcaat
    11161 tcctgaaagc gattgatatc gtgctggtgg cagtcagtga acacattgaa cgtttcgctg
    11221 ccctggcgcg tgaaatggcc gcgaccgaaa cccgcgaaag ccgtcgcgat gaactgctga
    11281 cgatagcaga aaactgcgat cttatcgccc accagccgcc gcagactttc tggcaggcgc
    11341 tgcaactgtg ttacttcatc cagttgattt tgcagatcga atctaacggt cactcggtat
    11401 cgtttggtcg tatggaccag tatctctatc catactatcg ccgtgacgtt gaactgaacc
    11461 agacactgga tcgcgaacac gccatcgaga tgctacatag ctgctggttg aaattgctgg
    11521 aagtgaacaa aatccgctcc ggctcacact caaaagcctc tgcgggaagt ccgctgtatc
    11581 agaacgtcac tattggcggg caaaatctgg ttgatggtca accgatggac gcggtaaatc
    11641 cactctctta tgcgatcctc gaatcttgcg gtcgcctgcg ttcgactcag cctaacctca
    11701 gcgtgcgtta ccatgcgggt atgagtaacg atttcctcga cgcctgtgtt caggtgatcc
    11761 gctgcggctt cgggatgccg gcgttcaaca acgacgaaat cgtcatcccg gaatttatta
    11821 aactcggtat tgaaccgcag gacgcttacg actatgcagc gattggttgt atcgaaaccg
    11881 ccgtcggtgg caaatggggc tatcgctgta ccggcatgag ctttatcaac ttcgcccgcg
    11941 tgatgctggc ggcgctggaa ggcggtcgtg atgccaccag cggcaaagtg ttcctgccac
    12001 aagaaaaagc gttgtcagcg ggtaacttca acaacttcga tgaagtaatg gacgcatggg
    12061 atacgcaaat ccgttactac acgcgtaaat caatcgaaat cgaatatgtc gtcgacacca
    12121 tgctggaaga gaacgtacac gatattctct gctcggcgct ggtcgatgac tgtattgagc
    12181 gagcgaaaag tatcaagcaa ggcggcgcga aatatgactg ggtttctggc ctgcaggtcg
    12241 gcattgctaa cctcggcaac agcctggcgg cagtgaagaa actggtattt gaacagggcg
    12301 cgattggtca gcaacagctg gctgccgcac tggcggatga cttcgacggt ctgactcacg
    12361 agcagctgcg tcagcggctg attaacggtg cgccgaagta cggcaacgac gatgatactg
    12421 tcgatacgct gctggctcgc gcttatcaga cctatatcga cgaactaaaa cagtaccata
    12481 acccgcgcta tggtcgtggt ccggttggcg gcaattatta cgcgggtacg tcatctatct
    12541 ccgctaacgt accctttggc gcgcagacga tggcaacgcc ggacggacgt aaagcgcata
    12601 ccccgctggc agaaggcgct agtccggcct ctggtactga ccatctcggc cctactgcgg
    12661 tcattggctc agtgggtaaa ctgcctacgg ccgcgattct cggcggcgtg ctgctcaacc
    12721 agaaactgaa tccagcaacg ctggagaacg aatctgacaa gcagaaactg atgatcctgc
    12781 tgcgcacctt cttcgaggtg cataaaggct ggcatattca gtacaacatc gtttcccgcg
    12841 aaacgctgct ggaagcgaaa aaacatccgg atcagtaccg cgatctggta gtgcgtgtcg
    12901 cgggctattc cgccttcttc accgcgctct ctccagacgc tcaggacgat atcatcgccc
    12961 gtactgaaca tatgctgtaa tctcctcagc ccggcggcgt cgtcgccggg ccaaatcatt
    13021 cacatcatca attccgctct cactttcatt cgaaatataa tttgtgctct gcgtcacatt
    13081 gttattacac tgttacggtt tcattttcga gccaggagca cacctatgag cgtaaaagtt
    13141 atcgtcacag acatggacgg tacttttctt aacgacgcca aaacgtacaa ccaaccacgt
    13201 tttatggcgc aatatcagga actgaaaaag cgcggcattg agttcgttgt cgccagcggt
    13261 aatcagtatt accagcttat ttcgttcttt cctgagctaa aggatgagat ctcttttgtc
    13321 gcggaaaacg gcgcactggt ttacgaacat ggcaagcagt tgttccacgg cgaactgacc
    13381 cgacatgaat cgcggattgt tattggcgag ttgctaaaag ataagcaact caattttgtc
    13441 gcctgcggtc tgcaaagtgc atatgtcagc gaaaatgccc ccgaagcatt cgtcgcactg
    13501 atggcaaaac actaccatcg cctgaaacct gtaaaagatt atcaggagat tgacgacgta
    13561 ctgttcaagt tttcgctcaa cctgccggat gaacaaatcc cgttagtgat cgacaaactg
    13621 cacatagcgc tggatggcat tatgaaaccc gtcaccagtg gttttggctt tatcgacttg
    13681 attattcccg gtctacataa agcaaacggt atttcgcggt tactgaaacg ctgggatctg
    13741 tcaccgcaaa atgtggtagc gattggcgac agcggtaacg atgcggagat gctgaaaatg
    13801 gcgcgttatt cctttgcgat gagcaatgct gcggaaaaca ttaaacaaat cgcccgttac
    13861 gctaccgatg ataataatca tgaaggcgcg ctgaatgtga ttcaggcggt gctggaaaat
    13921 aaaccgccgt ttaatctttg attatcacca attcagttta ttgagaaagt gcatgtcatt
    13981 ataccggata cggcgtgacc gccttatttg gcatacaaaa tcatgaaaat tcaatatatt
    14041 gcgatacact accaggcctg ataagcgtag cgcaccaggc aatcttgcgt ttgtcatcag
    14101 tttcagtagc cctgaccata tcagggctac tgataataac ttactcgatc cctttactac
    14161 gcaggtaatc ttcgtaatta ccgctaaagt cgatcacgcg ttccggggtg atttccagaa
    14221 tacgggtcgc cagggagctt acaaactcac ggtcgtgaga aacaaagatc agcgtgccct
    14281 gatacagttc cagcgccatg ttcagcgact caatggattc catatccagg tggttggtcg
    14341 gttcgtccat gatcagaatg ttcggcttct gcatcattaa cttaccaaac agcatccgcc
    14401 ctttttcccc accggaaagc actttagctg gctttttgat gtcgtcctgg ctgaacagca
    14461 aacgaccgag aatactgcgc accgcctgct cgtcatcgcc ttcctgcttc cactgactca
    14521 tccattcgaa cacggtcaga tcattttcaa actcatattc gtggtcctga gcgtagtaac
    14581 caatgcgcgc gttttcagac cattttacgg tgccgctgtc cggttgcaga tcgcccacca
    14641 gcgttttcag cagcgttgat ttaccgacgc cgttggtacc caataccgcc agtttttcac
    14701 ccacttccag cagcaggttg agatttttaa acagtggacc gttatcaaac cctttagtca
    14761 gaccttccac ttccagcgcg ttacggaaca gtttcttatc ctgttcaaaa cggatgaacg
    14821 ggttctgacg gctggaggct ttcacctctt ccagtttgat tttatcaatc tggcgcgcgc
    14881 gcgaagttgc ctggcgagat ttcgaggcgt tggcgctaaa gcggctaacg aaagattgca
    14941 actcagcaat ctgcgctttc ttcttggcgt tatcagccag cagacgttca cgcgcctgag
    15001 tcgccgccgt catgtactca tcgtagttgc ccggataaac gcgcagctcg ccgtaatcca
    15061 gatccgccat gtgggtacaa accatgttaa ggaagtgacg gtcgtgcgag atgatgatca
    15121 tggtgctgtc acgctcgttc agcacctgtt ccagccagcg aatggtgtcg atatcgaggt
    15181 tgttggtcgg ttcgtcgagc aggagaatgt ctggatcagc aaacagcgcc tgagccagca
    15241 gcacacgcag cttccagcca ggagcaactt cactcatcgg gccgtagtgc tgttccactg
    15301 gaattcccac gccaagcaac agttcaccag cgcgagcttc cgcagagtaa ccgtccattt
    15361 cgccgtattt cacttccaga tcggccactt tatagccgtc ttcttcactc atttccggca
    15421 aagcatagat gcggtcacgc tcctgcttca cttcccacaa ctctttatgc cccatgatca
    15481 ccgtatccag cacagtgaac tcttcaaagg caaactgatc ctgacgcagc ttaccaatgc
    15541 gctcgttggg atcgagggaa acgttaccca acgtcggctc taagtcgccg ccgaggatct
    15601 tcataaaggt ggatttacca ctaccgttcg cgccaatcag gccgtaacgg ttgccgccgc
    15661 cgaatttgac ggaaatgttt tcaaacaacg gcttactgcc gaactgcatg gtgacgttac
    15721 tggaaactaa cacaggcgta tcctgaaaag agatatgaca aaccgcgcat tatgccataa
    15781 gtctgaaaga agatcctgct cctcgcttaa ggtctacact caagccaaag gcaaaaaatg
    15841 tgatttcgta cacatctgat ttcactgtga gctggaatga acttataatg cgcttccagt
    15901 actctaatat tctcaaccca atggcctgcc aggcacaaaa tctcgcttaa catgaatatg
    15961 aaattgaaaa cattattcgc agcggccttc gctgttgtcg gcttttgcag taccgcctct
    16021 gcggtaactt atcctctgcc aaccgacggg agtcgcctgg ttggtcagaa tcaggtgatc
    16081 accattcctg aaggtaacac tcagccgctg gagtattttg ccgccgagta ccagatggga
    16141 ctttccaata tgatggaagc gaacccgggt gtggatacct tcctgccgaa aggcggtact
    16201 gtactgaaca ttccgcagca gctgatcctg ccggataccg ttcatgaagg catcgtcatt
    16261 aacagtgcag agatgcgtct gtattactat ccgaaaggga ccaacaccgt tatcgtgctg
    16321 ccgatcggca ttggtcagtt aggcaaagac acgcctatca actggaccac caaagttgag
    16381 cgtaaaaaag caggcccgac ctggacgccg accgctaaaa tgcacgcaga gtaccgcgct
    16441 gcgggcgaac cgcttccggc ggtcgttccg gctggtccgg ataacccgat ggggctgtat
    16501 gcactctaca tcggtcgcct gtacgctatc catggcacca acgccaactt cggtatcggc
    16561 ctgcgtgtaa gtcatggttg tgtgcgtctg cgtaacgaag acatcaaatt cctgttcgag
    16621 aaagtaccgg tcggtacccg cgtacagttt attgatgagc cggtaaaagc gaccaccgag
    16681 ccagacggca gccgttatat tgaagtccat aacccgctgt ctaccaccga agcccagttt
    16741 gaaggtcagg aaattgtgcc aattaccctg acgaagagcg tgcagacagt gaccggtcag
    16801 ccagatgttg accaggttgt tctggatgaa gcgatcaaaa atcgttccgg gatgccggtt
    16861 cgtctgaatt aatcttcaaa acgtaaagca aaggcggact cataatccgc ctttttaatt
    16921 tgacagacct tatcccgccg ggatcataac caacaaaaca tatcccacca gcgccgccca
    16981 caacagcatc ggtatcgaat aaagatgaaa acgccaccag atacggcgat cgttcgccat
    17041 ccgtaacgca atcaaatttg ccagcgatcc gggtaataaa ccaaagccac ctacgtttac
    17101 cgcccatgcc agtaataaag acggtggcac ataattgagc agcaaaatgg tactcggcac
    17161 gttactgatc acctgcgata aaccgatcgc cgttaaccat aatccgggtt cggataaatg
    17221 actcacgtta cttaacaccc cctgcaaaac tggcagttgg gttagtaaat ggacgtcgat
    17281 aaacatcgcc ataaacacca gcagcagcgt ccagtccaca ctgagcacca cgtgacgcgc
    17341 cagcagcgca aagcccgccg ccacaatcac cagtccccac agctcttgtt ttaactccag
    17401 cgccgtcaga aagacgatat acagccccag acaactccac accagccgcg gtttccactc
    17461 cggcgtttgc cctcccgtat ggtactgcag cgcctttcca gggaaacaac accagcacaa
    17521 cagcaggagt gtcagcatca ttgcgccagc cagcggtgcc atttgggcaa taaatccggc
    17581 aaacgaaaga ccagaacgtc cccagataag aatattttgc gggttgccaa ttggcgtcag
    17641 tagcgaacca gcgttgactg ccagcgcctc aaaaataatc agccgattaa ccgggatctc
    17701 acacagcctt tttagcgtga tagtcagcgg aacaactata aacagtgcga catcgttggt
    17761 cagaaaggta gaaagcagcg ccgccgccag taccataaac atcgccagcc gacgctccgt
    17821 agcaaaacgg cgcaccattt tgcgccccag cacatcaaaa taaccgctta actcgacacc
    17881 tttggtcagc agcatcaggc cgcttaaggt gatgatggtg tgccagtcga tagcagcagg
    17941 ccaggatttc ggtgcaaagg aaacgaaaaa acttaatccg atgccaacaa gaattaataa
    18001 ctgaaaaaaa cgatcgcctt gcagcgtgcg taaaaaaggc aggctcattt ggcaccgtgt
    18061 ttctgggtaa acaaacgaaa ggcatccagc gtctcttcac taacatggtg ctccatacct
    18121 tccgcgtcgc gacgggcgat ttccggactg acgcccaaca ccaacaagaa attttcgact
    18181 atctgatgtc gctcgcggct ttcctgcgcc agcttctctc cttctgccgt taaaaacacg
    18241 ccacgccagg ggatcatttc aatcagcccc atggttgcca gtcgcttaag cattttagcc
    18301 accgtcggtt gcgaaacccc cagacgagca gccatgtcta cctgacgagc ttcccccact
    18361 tccctgatca agtcagaaat cagctcaaca taatcatcaa taagctcgcg ccgatgcgcc
    18421 tctctgacct ggcggaaccc ttcaacgtgc tcttccacgt tcactaactg cgtcactttt
    18481 tttgctgttg gcgtacctgc gcgacgactc attgtgcttc ctcattatgg tgacgcatac
    18541 atagcgttct catttcgtgc gcacattgta aaccagagtt gcgaaggtac aaaaaattaa
    18601 cgttttagca atagctatat aatatagcct gtgctatatc tgtatgtaat gcaatcatcc
    18661 ctcaaggatc gatgggatta gcaagtcagg aggtcttatg aatgagttca agaggtgtat
    18721 gcgcgtgttt agtcattctc cctttaaagt acggttaatg ctgctctcta tgttgtgcga
    18781 tatggtcaac aacaaaccgc agcaggataa accttccgat aaatagcggc gtcgcggtac
    18841 gccgcttcac tcctgctttt atgcaggcac aacgcgtttt ggtctgaaaa accccacttt
    18901 ttgtcggatt tgcaatcccc ttcgcaaaag atttgttcgt cggtagttga cctgaacagc
    18961 agctcgctct atcttcttgc agccctgcgt atattgcggc tcgcggatgc ggaccccttt
    19021 cccctcttca cgcactcttg caggtattga cccttgacgc cagggtaagc acatggcgtt
    19081 tgttacgata gtggcatatg aatttaaccc tcaaagaatc gcttgttacc cgtagccggg
    19141 tatttagccc gtggactgcg ttctactttt tacagtcgct attaattaac ctcggcttag
    19201 gttacccctt cagtttgctc tacaccgctg cgtttacggc tattttgctt ttgctatggc
    19261 gaacattgcc tcgcgtacaa aaagttctgg tcggtatcag ttcgctggtg gcggcttgtt
    19321 atttcccttt tgctcaggcc tacggcgcgc ctaacttcaa tacattgctg gcattgcact
    19381 ccaccaatat ggaagagtcg accgaaatcc tgacgatttt tccgtggtac agctacctgg
    19441 tcggcatatt tatttttgcg ctcggcgtaa tagcaatcag gcgaaaaaaa gagagtgaaa
    19501 aagcgcgctg gaataccttc gacagcctgt gcctggtatt cagtgtggcg acattttttg
    19561 ttgctcccgt gcaaaacctg gcctggggcg gcgtatttaa actgaaagat actggctatc
    19621 cggtatttcg ttttgctaag gatgtcatcg tcaataataa cgaggtgatt gaagagcaag
    19681 aacggatggc aaaactttcc ggaatgaaag atacctggac ggtcactgcc gttaagccga
    19741 agtatcagac ctatgtggtg gtgatcggag aaagcgcgcg tcgcgatgcc ctcggtgcct
    19801 ttggcggtca ctgggacaat accccatttg ccagcagcgt taacggtttg atatttgctg
    19861 actacattgc cgccagtggc tccacgcaga aatcgcttgg cttaacgctc aatcgcgttg
    19921 tcgatggcaa accacagttt caggataact ttgtcaccct ggcaaatcgc gcgggcttcc
    19981 agacctggtg gttttccaac cagggccaaa tcggcgaata cgataccgcc atcgccagca
    20041 tcgccaaacg agcagatgaa gtgtacttcc tgaaagaagg taattttgaa gcagataaaa
    20101 acacgaaaga cgaagcgtta ctggatatga ccgctcaagt gctggcgcaa gagcactcgc
    20161 aaccgcagct gattgttcta catctgatgg gctcgcatcc gcaggcctgc gaccggacac
    20221 aaggcaaata cgaaaccttt gtgcaatcga aagaaacgtc gtgctatctc tataccatga
    20281 cgcaaacaga cgatttactg cgcaagctgt acgatcagtt acgcaacagc ggcagcagct
    20341 tctcgctggt ttacttttct gaccacggtc tggcctttaa agagcgcggc aaagacgtgc
    20401 aataccttgc tcatgatgat aagtatcagc aaaatttcca ggtgcctttt atggtcattt
    20461 ccagcgacga taaagcgcat cgggtgatta aagcccgccg ctcggccaat gacttcttag
    20521 gctttttctc gcagtggacg ggaattaaag cgaaggaaat aaatatcaaa tacccgttta
    20581 tatctgagaa gaaagccggg ccgatataca tcaccaactt ccagttacag aaggtggatt
    20641 acaaccatct cggaaccgat attttcgacc cgaaacctta aaacaaaaaa atccgccccg
    20701 agaggcggat ttttatatca ccgaagtgat tagaagcggt aaccaacacc ggcaatccag
    20761 gtgcctacgt caacgctacg aatacggctc tgctcgtaag agaagtccag agcaacgttt
    20821 tccatcgggt tgaactgcag accagcaccg taggagaaac cgtagtcgct ggtgtcgtgt
    20881 ttgtaggtcg ggtattcagt ggtctggaat ttaccataac ccacacccac tacaccgtag
    20941 atgcttgccc agtcgttaat gcggtaagcc ggaccagcag tgatgccgta gtactggttt
    21001 ttgttgtagt caccagagct tgcagtacgg cttttctcgg tgtaagtgaa agaaccgatc
    21061 acacccagcg ggctgttgtc ttcttcatag cggtatttca ggttgaaacc gcccattttg
    21121 ttcatttggc cctgagcgtc gctctgtgcg taaccgccag ttacagtaga agtcgcagct
    21181 acggaagtac ctgcggtgaa agccagaact gcggccagtg ctgaaagaca tgcaattttt
    21241 ttcataacca cctcaaatgt gattcaaata agtcctaagt tttaaatata tcaaaaatta
    21301 atgggaaact cttcgcgatt tatgatgtct aacgggccat ttcatgtaac agaacgtttc
    21361 catacaccgc tatccatcta aatttaaatc actttttcag agaactgcgt aagtattacg
    21421 catattttcc ctgtcattca tccagattat tcctaatcac cagactaatg attccatcaa
    21481 tcctggcgca ttttagtcaa aacgggggaa aattttttca acaaatgctc aaccagcatt
    21541 gggtatatcc agtacactcc acgctttact taggtttaga tatttgtggg agaaaggatg
    21601 cctggttcat tacgtaaaat gccggtctgg ttaccaatag tcatattgct cgttgccatg
    21661 gcgtctattc agggtggagc ctcgttagct aagtcacttt ttcctctggt gggcgcaccg
    21721 ggtgtcactg cgctgcgtct ggcattaggc acgctgatcc tcatcgcgtt ctttaagcca
    21781 tggcgactgc gctttgccaa agagcaacga ttaccgctgt tgttttacgg cgtttcgctg
    21841 ggtggaatga attatctttt ttatctttct attcagacgg taccgctggg tattgcggtg
    21901 gcgctggagt tcaccggacc actggcggtg gcactgttct cttctcgtcg cccggtagat
    21961 ttcgtctggg ttgtgctggc ggttcttggt ctgtggttcc tgctaccgct ggggcaagac
    22021 gtttcccatg tcgatttaac cggctgtgcg ctggcactgg gggccggggc ttgttgggct
    22081 atttacattt taagtgggca acgcgcagga gcggaacatg gccctgcgac ggtggcaatt
    22141 ggttcgttga ttgcagcgtt aattttcgtc ccaattggag cgcttcaggc tggtgaagca
    22201 ctctggcact ggtcggttat tccattgggg ctggctgtcg ctattctctc gaccgctctg
    22261 ccttattcgc tggaaatgat tgccctcacc cgtttgccaa cacgcacatt tggtacgcta
    22321 atgagcatgg aaccggctct ggctgccgtt tccgggatga ttttcctcgg agaaacactg
    22381 acccccatac agctgctggc gctcggcgct atcatcgccg cttcaatggg ttctacgctg
    22441 acagtacgca aagagagcaa aataaaagaa ttagacataa
//
LOCUS       DAHHTG010000072        22464 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.072, whole genome shotgun sequence.
ACCESSION   DAHHTG010000072 DAHHTG010000000
VERSION     DAHHTG010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22464)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22464)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22464
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.072"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            <1..880
                     /gene="xapR"
                     /locus_tag="NBI74_002767"
     CDS             <1..880
                     /gene="xapR"
                     /locus_tag="NBI74_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator XapR"
                     /protein_id="HCD1933074.1"
                     /translation="RVYRTDLKLLRYFLAVAEELHFGRAAARLNMSQPPLSIHIKELE
                     NQLGTQLFIRHSRSVVLTHAGKILMEESRRLLVNANNVLARVEQIGRGEAGRIELGVV
                     GTAMWGRMRPVMRRFLRENPNVEVLFREKMPAMQMALLERRELDAGIWRMATEPPTGF
                     TSLRLHESAFLVAMPEEHHLSSFSTVPLEALRDEYFVTMPPVYTDWDFLQRVCQQVGF
                     SPVVIREVNEPQTVLAMVSMGIGITLIADSYAQMNWPGVIFRPLKERIPADLYIVYET
                     QQVTPALVKLLAALAQ"
     gene            complement(991..1066)
                     /locus_tag="NBI74_002768"
     tRNA            complement(991..1066)
                     /locus_tag="NBI74_002768"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1031..1033),aa:Lys,seq:ttt)
     gene            complement(1071..1146)
                     /locus_tag="NBI74_002769"
     tRNA            complement(1071..1146)
                     /locus_tag="NBI74_002769"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1111..1113),aa:Val,seq:tac)
     gene            complement(1193..1268)
                     /locus_tag="NBI74_002770"
     tRNA            complement(1193..1268)
                     /locus_tag="NBI74_002770"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1233..1235),aa:Val,seq:tac)
     gene            complement(1313..1388)
                     /locus_tag="NBI74_002771"
     tRNA            complement(1313..1388)
                     /locus_tag="NBI74_002771"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1353..1355),aa:Val,seq:tac)
     gene            1647..3062
                     /gene="gltX"
                     /locus_tag="NBI74_002772"
     CDS             1647..3062
                     /gene="gltX"
                     /locus_tag="NBI74_002772"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HCD1933075.1"
                     /translation="MKIKTRFAPSPTGYLHVGGARTALYSWLFARNHGGEFVLRIEDT
                     DLERSTPEAIEAIMDGMNWLSLEWDEGPYYQTKRFDRYNAVIDQMLEEGTAYKCYCSK
                     ERLEALREEQMAKGEKPRYDGRCRHSHEHHADDEPCVVRFANPQEGSVVFDDQIRGPI
                     EFSNQELDDLIIRRTDGSPTYNFCVVVDDWDMEITHVIRGEDHINNTPRQINILKALK
                     APVPVYAHVSMINGDDGKKLSKRHGAVSVMQYRDDGYLPEALLNYLVRLGWSHGDQEI
                     FTREEMIKYFTLNAVSKSASAFNTDKLLWLNHHYINALPPEYVATHLQWHIEQENIDT
                     RNGPQLADLVKLLGERCKTLKEMAQSCRYFYEDFAEFDADAAKKHLRPVARQPLEVVR
                     DKLTAITDWTAENVHHAIQATADELEVGMGKVGMPLRVAVTGAGQSPALDVTVHAIGK
                     TRSIERINKALAFIAERENQQ"
     gene            complement(3113..3505)
                     /locus_tag="NBI74_002773"
     CDS             complement(3113..3505)
                     /locus_tag="NBI74_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416898.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding transcriptional regulator"
                     /protein_id="HCD1933076.1"
                     /translation="MKRLRNKMTTEELAECLGVAKQTVNRWIREKGWKTEKFPGVKGG
                     RARLILVDTQVCEFIQNTPAFHNTPMLLEAEEPLAEYAPGIRTPAYRQIISAIDNMTH
                     SEQEKVAQFLSREGIRNFLARLDIDESA"
     gene            complement(3507..3866)
                     /locus_tag="NBI74_002774"
     CDS             complement(3507..3866)
                     /locus_tag="NBI74_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding transcriptional regulator"
                     /protein_id="HCD1933077.1"
                     /translation="MFKERMTPDELARLTGYSRQTINKWVRKEGWTTSPKPGVQGGKA
                     RLVHVNEQVREYIRNAERPEGQGEAPALSGDAPLEVLLVTLAKEMTPVEQKQFTSLLL
                     REGIIGLLQRLGIRDSK"
     gene            4087..4162
                     /locus_tag="NBI74_002775"
     tRNA            4087..4162
                     /locus_tag="NBI74_002775"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4120..4122,aa:Ala,seq:ggc)
     gene            4202..4277
                     /locus_tag="NBI74_002776"
     tRNA            4202..4277
                     /locus_tag="NBI74_002776"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4235..4237,aa:Ala,seq:ggc)
     gene            4484..6673
                     /locus_tag="NBI74_002777"
     CDS             4484..6673
                     /locus_tag="NBI74_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing phosphodiesterase"
                     /protein_id="HCD1933078.1"
                     /translation="MFVEHNLIKNIKIFTLAFTLTVVLIQLSRLISPLAVIHSNYIFL
                     AWMPLCVMLSILFIFGWRGVVPILCGMFCTNLWNLHLTFLQTAVMIGSQAFAVLCACA
                     ILRWQLGTRWRYGLTSRYVWQRLFWLGLVAPIGIKCSMYLVGNFFDFPLKISTFFGDA
                     DAIFTVVDLLSLFTAVLIYNMLFYYLTRMIVSPHFAQILWRRDIAPSLSKEKRAFTLS
                     WLAALSVLLLLMCTPYENDFIAGYLVPVFFIIFTLGVGKLRYPFLNLTWAVSTLCLLN
                     YNQNFLQGVLTEYSLAFILAVLISFSVCLLYMVRIYHRSEWLNRRWHLQALTDPLTLL
                     PNFRALEQAPEQEAGKSFCCLRIDNLEFMSRHYGLMMRVHCIRSIYRTLLPLMQENEK
                     LYQLPGSELLLVLSGPETEGRLQHMVNILNSRQIHWNNTGLDMGYGAAWGHFDGNQET
                     LQPLLGQLSWLAEQSCAHHHVLALDSREEMVSGQTTKQVLLLNTIRTALDQGDLLLYA
                     QPIRNKEGEGYDEILARLKYDGGIMTPDKFLPLIAQFNLSARFDLQVLESLLKWLATH
                     PCDKKGPRFSVNLMPLTLLQKNIAGRIIRLFKRYYISPQAVILEITEEQAFSNAESSM
                     YNIEQLHKFGFRIAIDDFGTGYANYERLKRLQADIIKIDGVFVKDIVTNTLDAMIVRS
                     ITDLAKAKSLSVVAEFVETQQQQALLHKLGVQYLQGYLIGRPQPLAD"
     gene            complement(6723..7925)
                     /gene="nupC"
                     /locus_tag="NBI74_002778"
     CDS             complement(6723..7925)
                     /gene="nupC"
                     /locus_tag="NBI74_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupC"
                     /protein_id="HCD1933079.1"
                     /translation="MDRVLHFVLALAVVAILALLVSSDRKKIRIRYVIQLLVIEVLLA
                     WFFLNSDVGLGFVKGFSEMFEKLLGFANEGTNFVFGSMNDQGLAFFFLKVLCPIVFIS
                     ALIGILQHIRVLPVIIRAIGFLLSKVNGMGKLESFNAVSSLILGQSENFIAYKDILGK
                     ISRNRMYTMAATAMSTVSMSIVGAYMTMLEPKYVVAALVLNMFSTFIVLSLINPYRVD
                     ASEENIQMSNLHEGQSFFEMLGEYILAGFKVAIIVAAMLIGFIALIAALNALFATVTG
                     WFGYSISFQGILGYIFYPIAWVMGVPSSEALQVGSIMATKLVSNEFVAMMDLQKIAST
                     LSPRAEGIISVFLVSFANFSSIGIIAGAVKGLNEEQGNVVSRFGLKLVYGSTLVSVLS
                     ASIAALVL"
     gene            8261..9499
                     /locus_tag="NBI74_002779"
     CDS             8261..9499
                     /locus_tag="NBI74_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HCD1933080.1"
                     /translation="MTNYRVESSSGRAARKMRLALMGPAFIAAIGYIDPGNFATNIQA
                     GASFGYQLLWVVVWANLMAMLIQILSAKLGIATGKNLAEQIRDHYPRPVVWFYWVQAE
                     IIAMATDLAEFIGAAIGFKLILGVSLLQGAVLTGIATFLILMLQRRGQKPLEKVIGGL
                     LLFVAAAYIVELIFSQPNLAQLGKGMVIPSLPTSEAVFLAAGVLGATIMPHVIYLHSS
                     LTQHLHGGSRQQRYSATKWDVAIAMTIAGFVNLAMMATAAAAFHFSGHTGVADLDEAY
                     LTLQPLLSHAAATVFGLSLVAAGLSSTVVGTLAGQVVMQGFIRFHIPLWVRRTVTMLP
                     SFIVILMGLDPTRILVMSQVLLSFGIALALVPLLIFTSDSKLMGDLVNSKRVKQTGWV
                     IVVLVVALNIWLLVGTALGL"
     gene            complement(9640..9966)
                     /locus_tag="NBI74_002780"
     CDS             complement(9640..9966)
                     /locus_tag="NBI74_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein"
                     /protein_id="HCD1933081.1"
                     /translation="MFRSLFLAAALMAFTPLAANAGEITLLPSIKLQIGDRDHYGNYW
                     DGGHWRDRDYWHRNYEWRKNRWWRHDNGYHRGWDKRKAYERGYREGWRDRDDHRGKGR
                     GHGHRH"
     gene            complement(10081..11337)
                     /locus_tag="NBI74_002781"
     CDS             complement(10081..11337)
                     /locus_tag="NBI74_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ion channel protein"
                     /protein_id="HCD1933082.1"
                     /translation="MLHPRARTMLLLSLPAVAIGIASSLILIVVMKIASVLQNLLWQR
                     LPGTLGIAQDSPLWIIGVLTLTGIAVGLVIRFSQGHAGPDPACEPLIGAPVPPSALPG
                     LIVALILGLAGGVSLGPEHPIMTVNIALAVAIGARLLPRVNRMEWTILASAGTIGALF
                     GTPVAAALIFSQTLNGSNEVPLWDRLFAPLMAAAAGALTTGLFFHPHFSLPIAHYGQM
                     EMTDILSGAIVAAIAIAAGMVAVWCLPRLHAMMHQMKNPVFVLGIGGFILGILGVIGG
                     PVSLFKGLDEMQQMVANQAFSTSDYFLLAVIKLAALVVAAASGFRGGRIFPAVFVGVA
                     LGLMLHEHVPAVPAAITVSCAILGIVLVVTRDGWLSLFMAAVVVPNTTLLPLLCIVML
                     PAWLLLAGKPMMMVNRPKQQPPHDNV"
     gene            11541..12506
                     /gene="glk"
                     /locus_tag="NBI74_002782"
     CDS             11541..12506
                     /gene="glk"
                     /locus_tag="NBI74_002782"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HCD1933083.1"
                     /translation="MTKYALVGDVGGTNARLALCDIASGEISQAKTYSGLDYPSLEAV
                     IRVYLEEHKVEVKDGCIAIACPITGDWVAMTNHTWAFSIAEMKKNLGFSHLEIINDFT
                     AVSMAIPMLKKEHLIQFGGAEPVEGKPIAVYGAGTGLGVAHLVHVDKRWVSLPGEGGH
                     VDFAPNSEEEGIILEILRAEIGHVSAERVLSGPGLVNLYRAIVKADNRLPENLKPKDI
                     TERALADSCTDCRRALSLFCVIMGRFGGNLALNLGTFGGVFIAGGIVPRFLEFFKASG
                     FRAAFEDKGRFKEYVHDIPVYLIVHDNPGLLGSGAHLRQTLGHIL"
     gene            12725..13051
                     /locus_tag="NBI74_002783"
     CDS             12725..13051
                     /locus_tag="NBI74_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-like phosphotransferase enzyme IIB
                     component 1"
                     /protein_id="HCD1933084.1"
                     /translation="MSKKLIALCACPMGLAHTFMAAQALEEAAVEAGYEVKIETQGAD
                     GIQNRLTAQDIAEATIIIHSVAVTPEDNERFESRDVYEITLQDAIKNAAGIIKEIEEM
                     IASEQQ"
     gene            13073..14320
                     /locus_tag="NBI74_002784"
     CDS             13073..14320
                     /locus_tag="NBI74_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HCD1933085.1"
                     /translation="MAIKKRSATVVHGASGAAAAVKNPQASKSSFWGELPQHVMSGIS
                     RMVPTLIMGGVILAFSQLIAYSWLKIPADIGIMDALNSGKFSGFDLSLLKFAWLSQSF
                     GGVLFGFAIPMFAAFVANSIGGKLAFPAGFIGGLMSTQPTQLLNFDPSTMQWATSSPV
                     PSTFIGALIISIVAGYLVKWMNQKIQLPDFLLAFKTTFLLPILSAIFVMLAMYYVITP
                     FGGWINGGIRTVLTAAGEKGALMYAMGIAAATAIDLGGPINKAAGFVAFSFTTDHVLP
                     VTARSIAIVIPPIGLGLATIIDRRLTGKRLFNAQLYPQGKTAMFLAFMGISEGAIPFA
                     LESPITAIPSYMVGAIVGSTAAVWLGAVQWFPESAIWAWPLVTNLGVYMAGIALGAVI
                     TALMVVFLRLMMFRKGKLLIDSL"
     gene            14335..15420
                     /gene="ypdF"
                     /locus_tag="NBI74_002785"
     CDS             14335..15420
                     /gene="ypdF"
                     /locus_tag="NBI74_002785"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HCD1933086.1"
                     /translation="MTLLASLRDWLKAQQLDAVLLSSRQNKQPHLGISTGSGYVVISR
                     ESAHILVDSRYYADVEARTQGYQLHLLDAMHTLATIVRQIIADEQLQTLGFEGQQVSW
                     ETAHRWQSELNAKLVSATPDVLRQIKTPEEVEKIRLACGIADRGAEHIRRFIQAGMSE
                     CEIAAELEWFMRQQGAEKASFDTIVASGWRGALPHGKASDKIVAAGEFVTLDFGALYQ
                     GYCSDMTRTLLVNGEGVSAESHPLFNVYQIVLQAQLAAISVIRPGVRCQQVDDAARRV
                     ITEAGYGKFFGHNTGHAIGIEVHEEPRFSPRDTTTLQPGMLLTVEPGIYLPGQGGVRI
                     EDVVLVTPQGAEVLYAVPKTVLLTGEA"
     gene            15420..16457
                     /gene="ypdE"
                     /locus_tag="NBI74_002786"
     CDS             15420..16457
                     /gene="ypdE"
                     /locus_tag="NBI74_002786"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HCD1933087.1"
                     /translation="MDLSLLKALSEADAIASSEQEVRQILREEADRLQKEVRFDGLGS
                     VLIRLNESTGPKVMICAHMDEVGFMVRSISREGAIDVLPVGNVRMAARQLQSVRITTR
                     EECKIPGLLDSDRQGNDVSAMRVDIGARSCDEVMQAGIRPGDRVTFDTTFQVLPHQRV
                     MGKAFDDRLGCYLLVTLLRELHDAELPAEVWLVASSSEEVGLRGGQTATRAVSPDVAI
                     VLDTACWAKNFDYGAANHRQIGNGPMLVLSDKSLIAPPKLTAWIETVAAEIGVPLQSD
                     MFSNGGTDGGAVHLTGTGVPTVVMGPATRHGHCAASIADCRDILQMEQLLSALIQRLT
                     RETVVQLTDFR"
     gene            16482..18977
                     /gene="ptsP"
                     /locus_tag="NBI74_002787"
     CDS             16482..18977
                     /gene="ptsP"
                     /locus_tag="NBI74_002787"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HCD1933088.1"
                     /translation="MLTIQFLCPLPNGLHARPAWELKEQCSQWQSEITFINHRQNAKA
                     DAKSSLALIGTGTLFNDSCSLNISGSDEEQARRVLEEYIQVRFIDSDSVQPTLAELTA
                     HPLPRSLSRLNPDLLYGNVLASGVGVGTLTLLQSDSLDSYRVIPASAQDSTLLEHSLA
                     TLAEQLNQQLRERDGESKTILSAHLSLIQDDEFAGNIRHLMAEQHQGLGAAIISNMEQ
                     VCAKLSASASDYLRERVSDIRDISEQLLHITWPELKPRNNLVLEKLTILVAEDLTPSQ
                     FLSLDLKNLAGMILEKTGRTSHTLILARASAIPVLSGLPLDAIARYAGQPAVLDAQCG
                     VLAINPNDAVSGYYQVAQTLADKRQKQQAQAAAQLAYSRDNKRIDIAANIGTALEAPG
                     AFANGAEGVGLFRTEMLYMDRDSAPDEQEQFEAYQQVLLAAGDKPIIFRTMDIGGDKS
                     IPYLNIPQEENPFLGYRAVRIYPEFAGLFRTQLRAILRAASFGNAQLMIPMVHSLDQI
                     LWVKGEIQKAIVELKRDGLRHAETITLGIMVEVPSVCYIIDHFCDEVDFFSIGSNDMT
                     QYLYAVDRNNPRVSPLYNPITPSFLRMLQQIVTAAHQRGKWVGICGELGGESRYLPLL
                     LGLGLDELSMSSPRIPAVKSQLRQLDSEACRELARQACECRSAQEIEALLTAFAPEED
                     VRPLLALENIFVDQSFSNKEQAIQFLCGNLGVNGRTEHPFELEEDVWQREEIVTTGVG
                     FGVAIPHTKSQWIRHSSISIARLVKPVDWQSEMGEVELVIMLTLGANEGMNHVKVFSQ
                     LARKLVNKNFRQSLFAAQDAQSILTLLETELTF"
     gene            complement(18980..19837)
                     /locus_tag="NBI74_002788"
     CDS             complement(18980..19837)
                     /locus_tag="NBI74_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCD1933089.1"
                     /translation="MKAPGLPADQQFFADLFSGLVLNPQLLGRVWFASQPTSLPVGSL
                     CIDFPRLDIVLRGEYGNLLEAKQQRMVEGEMLFIPARAANLPVNNKPVLLLSLVFAPT
                     WLGLSFYDSRTTSLLHPARQIQLPSLQRGEGEAMLTALTHLSRSPLEQNIIQPLVLSL
                     LHLCRNVVNMPPGNSQPRGDFLYHSICNWIQDNYAQLLTRESVAQFFNITPNHLSKLF
                     AQHGTMRFIEYVRWVRMAKARMILQKYHLSIHEVAQRCGFPDSDYFCRVFRRQFGLTP
                     GEYSARFQG"
     gene            complement(19850..20584)
                     /gene="ypdB"
                     /locus_tag="NBI74_002789"
     CDS             complement(19850..20584)
                     /gene="ypdB"
                     /locus_tag="NBI74_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator YpdB"
                     /protein_id="HCD1933090.1"
                     /translation="MKVIIVEDEFLAQQELSWLIKEHSQMEIVGTFDDGLDVLKFLQH
                     NRVDAIFLDINIPSLDGVLLAQNISQFAHKPFIVFITAWKEHAVEAFELEAFDYILKP
                     YQESRITGMLQKLEAAWQQQQTSSTPAATVTRENDTINLVKDERIIVTPINDIYYAEA
                     HEKMTFVYTRRESYVMPMNITEFCSKLPPSHFFRCHRSFCVNLNKIREIEPWFNNTYI
                     LRLKDLDFEVPVSRSKVKEFRQLMHL"
     gene            complement(20599..22296)
                     /gene="ypdA"
                     /locus_tag="NBI74_002790"
     CDS             complement(20599..22296)
                     /gene="ypdA"
                     /locus_tag="NBI74_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     YpdA"
                     /protein_id="HCD1933091.1"
                     /translation="MHEIFNMLLAVFDRAALMLICLFFLIRIRLFRELLHKSAHSPKE
                     LLAVTAIFSLFALFSTWSGVPVEGSLVNVRIIAVMSGGILFGPWVGIITGVIAGIHRY
                     LIDIGGVTAIPCFITSILAGCISGWINLKIPKAQRWRVGILGGMLCETLTMILVIVWA
                     PTTALGIDIVSKIGIPMILGSVCIGFIVLLVQSVEGEKEASAARQAKLALDIANKTLP
                     LFRHVNSESLRKVCEIIRDDIHADAVAMTNTDHVLAYVGVGEHNYQNGDDFISPTTRQ
                     AMNYGKIIIKNNDEAHRTPEIHSMLVIPLWEKGVVTGTLKIYYCHAHQITSSLQEMAV
                     GLSQIISTQLEVSRAEQLREMANKAELRALQSKINPHFLFNALNAISSSIRLNPDTAR
                     QLIFNLSRYLRYNIELKDDEQIDIKKELYQIKDYIAIEQARFGDKLTVIYDIDEEVNC
                     CIPSLLIQPLVENAIVHGIQPCKGKGVVTISVAECGNRVRIAVRDTGHGIDPKVIERV
                     EANEMPGNKIGLLNVHHRVKLLYGEGLHIRRLEPGTEIAFYIPNQRTPVASQATLLL"
BASE COUNT         5352 a         5546 c         5998 g         5568 t
ORIGIN      
        1 acgcgtatat agaacagatc ttaagttgct ccgttatttt cttgccgtag cggaagagtt
       61 gcattttggt cgcgcagcag cgcgtttaaa tatgtctcag cctccgctca gtattcatat
      121 taaagagctg gaaaatcaac tcggcacgca gctttttatt cgccattcgc gcagcgtcgt
      181 actgacacac gcgggcaaaa tcttgatgga agaatcgcgt cgattgctgg tgaatgcaaa
      241 taatgtattg gctcgggttg aacaaatagg tcggggagaa gcagggcgga ttgaactcgg
      301 ggttgtggga acggcaatgt ggggacggat gcgcccggtt atgcggcgat tccttagaga
      361 aaatcctaac gttgaagttc tgtttcgcga aaagatgccc gcgatgcaaa tggccttgct
      421 ggaacgccgc gaacttgatg ccgggatctg gcgaatggcg acagaaccac cgactggttt
      481 taccagctta cggttgcatg aatcggcgtt tctggtggcg atgcctgaag agcatcatct
      541 ctcatcattt tccaccgtcc cgctggaagc gctacgtgac gagtatttcg ttacaatgcc
      601 gcccgtgtac actgactggg attttttgca gcgagtttgc cagcaggtgg gattttcacc
      661 ggttgttatt cgcgaagtta atgaaccgca aacggtactc gccatggtca gtatgggtat
      721 tggtatcaca ttgatagcgg acagctacgc gcagatgaac tggcccggtg tcatattccg
      781 tccgctcaaa gaacgcatcc cggcagactt atatattgtt tatgaaacgc agcaggtgac
      841 gcccgcgctg gttaaactgc tggcggcgtt ggcgcagtag atgagaggca cgggaactgc
      901 cggacatagt tggtctgata agcgaagcgc atcaggcaat ttcgcaaatt gcagatagca
      961 aaaaaacgcc tttagggcgc ttttttacat tggtgggtcg tgcaggattc gaacctgcga
     1021 ccaattgatt aaaagtcaac tgctctacca actgagctaa cgacccgaag tggtgggtga
     1081 tgacgggatc gaaccgccga ccccctcctt gtaagggagg tgctctccca gctgagctaa
     1141 tcacccactt cggtacttca cattgttaca agaaatctag ctggcgagaa agtggtgggt
     1201 gatgacggga tcgaaccgcc gaccccctcc ttgtaaggga ggtgctctcc cagctgagct
     1261 aatcacccac ttctcaattt cttgctacac ggcggagact acataaagta gttggtgggt
     1321 gatgacggga tcgaaccgcc gaccccctcc ttgtaaggga ggtgctctcc cagctgagct
     1381 aatcaccccc gctgtgtgga gtcgcattat agggagagtt caaaatgagt caacgcattt
     1441 tctaaagaaa ttgttcgttc gtcgtaaatt taagcaagat gatcgcaaaa cagaccgtgt
     1501 tgcgcaattt gtcaacgaaa acaataatgc gtaaggttgc aacccgaact acattgagga
     1561 atcaggcggg agtgatagaa tatcgcccac tcaatttttc caggatttgc cggttgtcgg
     1621 catctttcta aacgtaaggc catttcatga aaatcaaaac tcgcttcgcg ccaagcccaa
     1681 caggctatct gcacgttggc ggcgcgcgta ctgctcttta ctcctggctt tttgcacgta
     1741 accacggcgg tgagttcgtg ctgcgtattg aagacaccga tcttgagcgt tccacgccgg
     1801 aagctatcga agccattatg gatggcatga actggctgag cctggagtgg gatgaaggtc
     1861 cgtactacca gaccaaacgt tttgatcgct acaacgcggt gatcgatcag atgctggaag
     1921 agggcacagc ttataaatgc tattgctcta aagagcgcct ggaagcgctg cgcgaagagc
     1981 aaatggcgaa aggtgagaag ccgcgttatg acggtcgctg ccgccacagt catgagcatc
     2041 acgctgatga tgagccgtgt gtcgtacgtt ttgctaaccc gcaggaaggt tctgttgttt
     2101 ttgacgatca gatccgtggt ccgatcgagt tcagcaacca ggagctggat gatctgatta
     2161 tccgccgtac cgatggttcc ccaacctata acttctgtgt ggttgtcgat gactgggata
     2221 tggaaatcac ccacgttatc cgtggcgaag accatatcaa caacacaccg cgccagatta
     2281 acattcttaa ggctctgaaa gcgccggtac cggtttatgc gcacgtttct atgatcaacg
     2341 gcgatgacgg taaaaaactg tccaaacgtc acggggcggt cagcgtaatg cagtatcgtg
     2401 atgatggtta tctgccagaa gcactgctga actatctggt gcgtctgggc tggtcccacg
     2461 gcgatcagga aatcttcact cgtgaagaga tgattaaata cttcactctg aatgccgtca
     2521 gcaaatctgc cagtgcgttc aacaccgaca agctgctgtg gctgaaccat cactacatta
     2581 acgcgttgcc gccggagtat gttgctactc acttacagtg gcacattgag caggaaaata
     2641 tcgatacccg taacggcccg cagctggctg atctggtgaa actgttgggc gaacgctgca
     2701 agacgctgaa agagatggca cagagctgcc gttatttcta cgaagatttt gctgagttcg
     2761 atgccgacgc cgcgaaaaaa catctgcgtc cggtagcgcg ccagccgctg gaagtggttc
     2821 gtgacaaact gactgcgatt actgactgga ccgctgaaaa cgttcatcac gctattcagg
     2881 cgacggcgga tgagctggaa gtgggtatgg gtaaagttgg tatgccgctg cgtgtcgccg
     2941 taaccggtgc ggggcagtct ccggcgctgg atgttaccgt tcacgcaatc ggtaagaccc
     3001 gcagtatcga gcgtatcaac aaagcgctgg cttttattgc tgaacgcgaa aatcagcagt
     3061 aattagcgca taaaagataa acggcaggag agatacccct gccgtttttt atttatgctg
     3121 attcatctat gtcgagacga gcaagaaaat tacgaattcc ttcgcgtgac aaaaattgcg
     3181 caactttttc ttgctcactg tgagtcatgt tgtctattgc gctgatgatt tgccgataag
     3241 caggagtgcg aatacccggt gcgtattcgg caaggggttc ttcggcttcc agaagcatcg
     3301 gtgtgttatg gaaggctggc gtgttctgaa taaactcgca aacttgcgta tcgaccagaa
     3361 taagtctggc acgaccgcct ttcacgccgg gaaatttttc cgttttccag cctttttctc
     3421 tgatccaacg gttaacagtt tgtttagcca caccgaggca ttcagccagt tcttcggtgg
     3481 tcattttatt gcgtaatctt ttcatattat ttgctatcgc gaatccctaa gcgttgtaac
     3541 aatccgataa tcccttcccg cagaagcaag gatgtaaact gtttttgttc aactggcgtc
     3601 atctctttcg ccagtgtcac cagcaacact tcaagcggcg catcaccaga aagggctggc
     3661 gcttctccct gaccttctgg acgttcagca ttgcgaatat attcacgaac ctgttcattg
     3721 acgtgaacca gtcgagcctt gccaccctgg acgcctggtt ttggtgacgt tgtccagcct
     3781 tccttgcgta cccatttatt aatggtctgg cggctatagc cggtcagtct ggcaagttca
     3841 tctggcgtca tcctctcctt gaacataaca attccctgaa tgtatgtggg tctattagta
     3901 gttcatttta taacaccact ttacaggaaa ccgtgacatg aataactact gaatgcgagc
     3961 ggttttccaa aattgttcat ttgcctgatt tttcagcgat tgaaactaca cgtggcaatt
     4021 tgccgttgac acattcgggc ggaattcata tgatgccgcc cgtcaactcg acaagcttta
     4081 cgtgatgggg ctatagctca gctgggagag cgcttgcatg gcatgcaaga ggtcagcggt
     4141 tcgatcccgc ttagctccac caaattttgc acccagcaaa tttggtacgt aaacgactcg
     4201 tggggctata gctcagctgg gagagcgctt gcatggcatg caagaggtca gcggttcgat
     4261 cccgcttagc tccaccaaat ttcaaaccct cgctgcaaag cgggggtttt ttgtctctgc
     4321 cttttgccgc ttttgtaata cagtctacat cctggcagtg ccgtgtggtg aatgcatcac
     4381 tgactgcaaa aacagcaaca ggctggcccc tgtttgcttc gcgatgcgaa taaacttatt
     4441 atttgtgtgc ctgaaaaccc cgatcagtga gagtagtgta ctcatgtttg tggagcataa
     4501 cctgataaaa aatatcaaga tattcacact agcgtttacg ctcaccgtgg tacttattca
     4561 gctatcccgt ttaatttcgc cacttgctgt tatccattcc aattatatct ttctggcgtg
     4621 gatgccactg tgcgtaatgc tgtcaatctt gtttatcttt ggctggcgtg gtgtcgttcc
     4681 cattttatgt gggatgtttt gcaccaatct gtggaacctt catctcactt ttttacagac
     4741 cgccgtcatg atcggcagcc aggcgtttgc agtcctgtgt gcctgtgcaa tattacgctg
     4801 gcaattgggg acgcgttggc gttatggatt gaccagccga tacgtctggc aacgtctgtt
     4861 ctggcttggt ttggtggcgc cgattggcat caaatgcagc atgtatcttg tgggaaattt
     4921 ctttgatttt ccgctaaaga tatctacctt tttcggcgat gcggatgcca ttttcacggt
     4981 cgttgatttg ctaagccttt tcaccgcagt gctgatttac aacatgcttt tttactatct
     5041 cacccgcatg attgtaagtc cccactttgc acagatattg tggcgtaggg atatcgctcc
     5101 gtcgttgagc aaagagaaac gcgcatttac cttaagctgg ctggcagctc ttagcgtgct
     5161 gctacttctg atgtgcacac cgtatgaaaa tgactttatt gccggttact tggtacctgt
     5221 tttctttatc atctttaccc tcggtgtcgg taagcttcgc tatccgtttt taaatctcac
     5281 ctgggctgtt tcaacgcttt gcctgctgaa ttacaaccag aactttttgc aaggggtact
     5341 aaccgaatat tcgctggcat ttattctcgc agtactgatt tcctttagtg tttgtctgct
     5401 ctatatggtg cgtatttatc atcgtagtga atggcttaac cgccgctggc atttgcaggc
     5461 gctgaccgat ccgttaacgc tcctacccaa ctttcgtgcg ttggaacaag caccggagca
     5521 agaggcgggc aagagttttt gctgcctgcg cattgataat cttgagttta tgagtcgtca
     5581 ttacggctta atgatgcgcg ttcactgtat ccgctcaatt taccgtacgt tgctgccgtt
     5641 gatgcaggaa aacgaaaagt tgtatcaatt gccgggtagt gaactgctgt tagtgctgag
     5701 cgggccggaa acggaagggc gactccagca tatggttaac atcctgaata gtcggcaaat
     5761 tcactggaac aataccgggc tggatatggg ctatggtgct gcctgggggc attttgatgg
     5821 aaatcaggaa accctgcaac ccttgttggg gcagttaagc tggctggcgg agcaatcctg
     5881 cgcacatcat catgtgctgg cgctggatag cagagaggag atggtttccg ggcaaaccac
     5941 taaacaggtg ctattgctga ataccattcg cacggcgtta gatcagggtg atttgctgct
     6001 ctacgcccag ccaattcgca acaaagaggg tgaaggttat gatgagatcc tcgcgcgact
     6061 gaagtatgac ggcggcatta tgaccccgga taagtttctg ccccttattg ctcagtttaa
     6121 ccttagcgcg cgttttgatt tgcaagtgct ggaatccttg ttgaagtggc tggcaacaca
     6181 cccttgcgac aaaaaaggtc cacgattttc agtcaattta atgccgctca cgctgctgca
     6241 aaagaatatt gccgggcgga ttattcgtct gtttaagcgt tattacatct ccccgcaggc
     6301 ggtcattctt gagatcaccg aggagcaggc gttttctaac gcagaaagca gcatgtacaa
     6361 catcgagcag ctgcataagt ttggtttccg gattgcgatt gatgactttg gcaccggata
     6421 tgccaactac gaacggttaa agcgtttgca ggctgatatc atcaaaattg atggcgtctt
     6481 tgtgaaagat atcgtcacga acacgctgga tgcgatgatt gtgagatcaa ttaccgatct
     6541 ggcgaaagcg aagtcattga gtgtggtcgc ggagtttgtc gagacgcaac agcagcaggc
     6601 gctattgcat aagctcgggg tgcaatatct gcaagggtat ttgattggtc gcccgcagcc
     6661 attagccgat taacaggcgt aaaaaagccg gggaattatc cccggctttt ttatgtatgg
     6721 tcttacagca ccagtgctgc gattgacgca gacagcacac tcaccagggt agagccgtaa
     6781 accagtttca gaccgaagcg agaaaccacg ttaccttgct cttcattcag gcctttaacc
     6841 gcacccgcga taatcccgat tgaagagaag ttagcgaagg aaaccaggaa cacagagatg
     6901 atgccttccg cacgcggaga gagcgtggaa gcaattttct gcagatccat catcgcaacg
     6961 aactcgttgg aaaccagttt ggtcgccatg atactgccca cttgcagtgc ttcactggaa
     7021 ggaacaccca tcacccatgc aatcggatag aagatgtaac ccaggatgcc ctggaaggag
     7081 atgctgtagc caaaccagcc agtcacggtg gcaaacagtg cgttcagcgc ggcgatcagg
     7141 gcgataaagc cgatcagcat cgccgcaacg ataatggcaa ccttgaaacc tgccagaatg
     7201 tattcaccca gcatttcgaa gaagctctga ccttcgtgca ggttggacat ctgaatattt
     7261 tcttcactgg catcaacacg gtaagggttg atcagcgaca gcacgataaa ggtgctgaac
     7321 atgttcagta ccagcgccgc aacgacgtat ttcggctcca gcatggtcat gtatgcacca
     7381 acgatggaca tcgacacggt ggacatcgcc gttgctgcca tggtgtacat acgattgcgg
     7441 gagattttgc cgaggatatc tttataggca ataaagtttt cagactgacc cagaatcagg
     7501 gagctgacgg cgttaaagga ttccagtttg cccatgccgt tgactttgga gagcaggaaa
     7561 ccaattgcgc ggataatcac cggcaacacg cgaatgtgct ggagaatacc gatcagtgca
     7621 gagataaaga cgattgggca cagcactttc aggaagaaga atgccaggcc ttgatcattc
     7681 atgctaccaa agacgaagtt agtcccttcg ttggcaaaac cgagcagttt ttcgaacatt
     7741 tcggagaagc ctttcacgaa gcccagacca acgtcggagt tcaggaagaa ccacgccagt
     7801 aacacttcga taacaagcag ttgaataaca taacggatac gaattttttt gcggtcgctg
     7861 cttaccagca gtgcgagaat cgcaacaacg gcaagtgcca gtacaaaatg aaggacgcgg
     7921 tccatatttg ctccaaatat gaggcaggtt aaatttccgt gcacattcta tgtaacatgg
     7981 ttaaggaaaa cgagatctga cacacactat aatgacgtcc tgtgacaagc ttcaaaaata
     8041 gtgatctgtc atacatttct gctatgttgt gtatggaagc ggaaggttat gtaaatgtgc
     8101 taatattaaa cataaccata acattcgagt catctatctt ttcgtagtca taatcctggt
     8161 ctatcagaga aatcatcaca acccatttaa atgaatttga taatcattct catttaacgt
     8221 agcatgaaac atagcaaagg ctatgtttta gaggcacaag atgacgaact atcgcgttga
     8281 gagtagcagc ggacgggcgg cgcgcaagat gaggctcgca ttaatgggac ctgcgttcat
     8341 tgcggcgatt ggttatatcg atcccggtaa ctttgcgacc aatattcagg cgggtgccag
     8401 cttcggctat cagctactgt gggttgtcgt ttgggctaac ctgatggcga tgctgattca
     8461 aatcctctct gccaaactag ggattgccac cggtaaaaat ctggcggagc agattcgcga
     8521 tcactatccg cgtcccgtag tgtggttcta ttgggttcag gcagaaatta ttgcgatggc
     8581 aaccgacctg gcggaattta ttggtgcggc gatcggtttt aaactcattc ttggtgtttc
     8641 gttgttgcag ggcgcggtgc tgacggggat cgcgactttc cttattttaa tgctgcaacg
     8701 tcgcgggcaa aaaccgctgg agaaagtgat tggcgggtta ctgttgtttg ttgccgctgc
     8761 ttacattgtc gagttgattt tctcccagcc taacctggcg cagctgggta aaggaatggt
     8821 gatcccgagt ttacctactt cggaggcggt cttcctggcc gcgggcgtgt taggggcgac
     8881 gattatgccg catgtgattt atttgcactc ttcgctcact cagcatttac atggcggttc
     8941 gcgtcaacaa cgttattccg ccaccaaatg ggatgtggct atcgccatga cgattgccgg
     9001 ttttgttaat ctggcgatga tggctacagc tgcggcggcg ttccactttt ctggtcatac
     9061 tggtgttgcc gatcttgatg aggcttatct gacgctgcaa ccgctgttaa gccatgctgc
     9121 ggcaacggtc tttggattaa gcctggttgc tgccgggttg tcctcaacgg tggtggggac
     9181 gctggcgggg caggtagtga tgcagggctt cattcgcttt catatcccgc tgtgggtgcg
     9241 tcgtactgtc accatgctgc cgtcatttat tgtcattctg atgggattag atccgacacg
     9301 gattctggtt atgagtcagg tgctgttaag ttttggtatc gccctggcgc tggttccact
     9361 gttgattttc accagtgaca gcaagttgat gggcgatctg gtgaacagca aacgcgtaaa
     9421 acagacaggc tgggtgattg tggtgctggt agtggcgctg aatatctggt tactggtagg
     9481 aacggcactg ggattgtagt tgaatgagcg tcgcatttgg caccattggc ggatgcggcg
     9541 taaatgcttt atccgcccta cgcgttaaag agctggtttg taggcctgat aagacgcgct
     9601 agcgtcgcat caggcattgt gctccattgg cgaatgtgtt taatggcggt gtccatgtcc
     9661 gcggcctttt ccacgatgat cgtcacgatc gcgccagcct tcacgatagc cacgctcata
     9721 cgctttacgc ttatcccagc cacgatggta gccattatca tgacgccacc agcggttttt
     9781 acgccattca taattgcggt gccagtagtc acggtcgcgc cagtgaccac cgtcccagta
     9841 attaccgtaa tgatcgcgat cgccaatttg taatttgatt gatggcagta gggtgatttc
     9901 acctgcgttt gccgcaagcg gggtaaatgc catcagggcg gccgccagaa acagtgacct
     9961 gaacattgtt attctccttc acgatcgaag ccgtcagcgg cctgttaacg caatattacg
    10021 gggaggtaaa gcgccgtatc atcgccataa ctcttaaatc ataaaggaga gcattttttg
    10081 ctaaacgtta tcgtggggtg gctgttgctt cggacgattg accatcatca tcggcttacc
    10141 tgctaataac agccatgccg ggagcatgac gatacagagc agcggtagca atgtggtatt
    10201 gggtacaacg actgccgcca taaaaagact taaccagcca tcacgtgtta ccaccagcac
    10261 gatgccgaga atagcgcagg aaacggttat tgccgctgga acggcgggta cgtgctcatg
    10321 cagcatcaac cctaatgcca cgccgacaaa cactgccggg aagattcgcc caccgcgaaa
    10381 gccactggcg gcagcaacga ccagggcggc aagttttatc accgccagca aaaagtaatc
    10441 gctggtgctg aaagcctgat ttgccaccat ctgctgcatc tcatccagcc ctttaaacag
    10501 cgaaaccggt ccaccaataa cccccagaat accgagaata aatccgccaa tacccagcac
    10561 gaacaccgga tttttcattt gatgcatcat cgcgtgcaac cgtggtaagc accatacggc
    10621 gaccatccct gctgcgatgg caatcgccgc gacaattgca ccgctgagaa tatcggtcat
    10681 ttccatctgt ccgtaatgcg caatgggcag tgaaaaatga ggatggaaaa ataatccggt
    10741 agtaagtgca ccagctgctg ccgccattaa cggcgcaaag agacgatccc atagcggaac
    10801 ttcattactg ccatttaagg tttgcgaaaa tatcaatgcc gccgcaacag gtgtgccaaa
    10861 cagcgcgccg atggttccgg cagaggctaa aatagtccac tccattcggt tgacgcgcgg
    10921 taacagacga gcgccaatgg cgacggcaag ggcgatattg acggtcatga tcggatgttc
    10981 cggccccagg ctgacgccgc cagcaagacc gagaattaat gctacgataa gtcctggtag
    11041 cgcagagggc ggaaccggtg cgccgatcag cggttcacag gcggggtctg gtccggcatg
    11101 accctggctg aaacggataa ccaaccccac cgcaataccc gttagcgtta atacaccgat
    11161 gatccagaga ggtgaatcct gggctatccc cagagttccc ggcagtcgtt gccagagcaa
    11221 attctgtaat accgaggcga ttttcatcac cacaatcaga ataagactgg acgcaatccc
    11281 aattgccacg gcggggagcg ataataacaa catggttctg gctcgcggat ggagcatgtt
    11341 ctattcctta tgcggggtca gatacttagt ttgcccagct tgcaaaaagg catcgctgca
    11401 attggtgctg aaacgataaa gtaattgtgt gacccagatc gatatttaca gggagcctgc
    11461 ctttccggcg ttgttgttat gcccccaggt atttacagtg tgagaaagaa ttattttgac
    11521 tttagcggag cagttgaaga atgacaaagt atgcattagt cggtgatgtg ggcggcacca
    11581 acgcacgtct tgctctgtgt gatattgcca gtggtgaaat ctcgcaggct aagacctatt
    11641 cagggcttga ttaccccagc ctcgaagcgg tcattcgcgt ttatcttgaa gaacataagg
    11701 tcgaggtgaa agacggctgt attgccatcg cttgcccaat taccggtgac tgggtggcaa
    11761 tgaccaacca tacctgggcg ttctcaattg ccgaaatgaa aaagaatctc ggttttagcc
    11821 atctggaaat tattaacgat tttactgctg tgtcgatggc gatcccgatg ctgaaaaaag
    11881 agcatctgat tcagtttggt ggcgcagaac cggtagaagg taagcctatt gcggtttacg
    11941 gtgccggaac agggcttggg gttgcgcatc tggtccatgt cgataagcgt tgggtaagct
    12001 tgccaggaga aggcggtcac gttgattttg cgccgaatag tgaagaagag ggcattatcc
    12061 tcgaaatatt gcgtgcggaa attggtcatg tttcggcgga acgcgtgctt tctggccctg
    12121 ggctggtgaa tttgtatcgc gcaattgtta aagccgacaa ccgcctgcca gaaaatctca
    12181 agccaaaaga tattaccgaa cgcgcgctgg ctgacagctg caccgattgc cgccgcgcgt
    12241 tgtcgctgtt ttgcgtcatt atgggccgtt ttggcggcaa tctggcgctc aatctcggga
    12301 catttggcgg cgtgtttatt gcgggtggta tcgtgccgcg ctttcttgag ttcttcaaag
    12361 cctctggttt ccgcgccgca tttgaagata aagggcgctt taaggaatat gtccatgata
    12421 ttccggtata tctcatcgtc catgacaatc cgggtcttct cggttccggt gcacatttac
    12481 gccagacctt aggtcacatt ctctaaatcc ttcctgttat atcgggaggt aactctcccg
    12541 ataatctttt aaatcataca gttaattaaa tttttctttg tgtcccctca caaggccgac
    12601 ctgcgtcaca cttccgtaca gcgggattaa ttctccagta aatgcattat ttgtctggta
    12661 acggcgattt gttttgcacg ttcataattt cactcgtcaa ctatcactga acgaggaatt
    12721 aacgatgagt aagaaactga ttgccttatg tgcctgccca atgggcctgg ctcacacttt
    12781 tatggccgct caggcgctgg aagaagcggc ggtagaagcc ggttatgaag tgaaaattga
    12841 aacccagggc gcggacggta tccagaatcg cctgacggcg caggatatcg ccgaagcgac
    12901 catcatcatc cactccgtgg cagttacccc ggaagataac gagcgtttcg aatcacgcga
    12961 cgtttatgaa atcactttgc aggacgcaat taaaaacgct gcgggaatca tcaaagaaat
    13021 cgaagagatg attgcctctg aacaacagta atattcgaca gggataattg ttatggccat
    13081 taaaaaacgc agtgcaaccg ttgtgcatgg cgcatccggt gcggcagcgg cagttaagaa
    13141 tccgcaggcc tctaaatcca gcttctgggg tgaactcccg cagcatgtga tgtcagggat
    13201 ttcacgcatg gtgccgacct taattatggg cggtgtgatc ctcgctttca gtcagctgat
    13261 tgcttatagc tggcttaaaa ttcctgctga tataggcatc atggatgctc ttaatagcgg
    13321 gaaattctcc ggtttcgacc tctccttact taaatttgcc tggctgtcgc agtccttcgg
    13381 cggcgtgttg tttggttttg caattccgat gtttgctgct tttgtggcga actctatcgg
    13441 tggcaaactg gcgttcccgg ctggttttat cggcggcttg atgtctaccc agccgacgca
    13501 actgctgaac ttcgatccca gcactatgca atgggcgacc tcttcgccgg tgccgtccac
    13561 cttcattggt gcgctgatta tttctatcgt tgccggttac cttgtgaaat ggatgaacca
    13621 gaaaatccag ttaccggatt tcctgttagc gttcaaaacc acatttttgc taccgattct
    13681 ttccgccatt tttgtcatgc tggcgatgta ctacgtcatc accccctttg gtggctggat
    13741 caacggcggt atccgtaccg tgctgaccgc tgcaggtgag aagggcgcac tgatgtacgc
    13801 aatggggatc gccgcggcga cagcaatcga ccttggtggc ccgatcaaca aagcggcagg
    13861 tttcgttgcc ttcagcttta ccactgacca cgtattaccg gtcactgcac gttctatcgc
    13921 tatcgttatt ccgccgattg gtctgggtct ggcgaccatt attgaccgtc gtttaaccgg
    13981 caaacgcttg ttcaacgccc agctttatcc gcagggtaaa accgccatgt tcctggcgtt
    14041 tatggggatc agtgagggtg cgattccgtt tgcactggaa agccctatca ccgccattcc
    14101 gtcgtatatg gtcggcgcga ttgtcggctc aaccgccgct gtctggctgg gcgcagtgca
    14161 atggttcccg gagtccgcta tctgggcatg gccgctggtc actaacctcg gcgtctacat
    14221 ggcggggatc gcgctggggg cagtcataac tgcgctgatg gtggtgttcc tgcgtctgat
    14281 gatgttccgt aaaggcaaat tgttaatcga tagcctgtaa gaaggacaat cctgatgaca
    14341 ttactcgctt cgctgcgcga ctggcttaag gcgcaacaac tggatgcggt gcttctctcc
    14401 tcacggcaga acaaacagcc gcatctgggg atctccaccg gatcaggtta tgtggtgatt
    14461 agccgtgaaa gtgcgcacat tctggtggat tcgcgctatt acgcggatgt agaagcccgc
    14521 acgcaaggtt accagctgca tttgctcgac gcgatgcaca cgcttgccac catcgtcaga
    14581 caaatcattg ccgatgagca gttgcaaacg ctcggttttg aaggccagca ggtgagttgg
    14641 gaaaccgcgc accgctggca gtccgaactc aatgcgaaac tggtgagcgc cacgccggat
    14701 gtgctgcggc aaatcaaaac gccagaagag gtggagaaaa tccgccttgc ctgcgggatt
    14761 gccgatcgtg gcgcagagca tattcgccgc tttattcagg cgggaatgag cgaatgcgag
    14821 atagccgctg aactggagtg gtttatgcgc cagcagggcg cagaaaaagc ctcttttgac
    14881 accattgtcg ccagcggctg gcgtggggcg ctgccgcacg gcaaagccag cgacaagatt
    14941 gttgcagcgg gcgagtttgt cactctcgat ttcggtgcgc tgtatcaggg ctactgctct
    15001 gatatgacgc gcaccttgct ggtgaatggc gaaggggtga gcgccgaatc tcatccgctg
    15061 tttaacgtct atcagattgt cctgcaggca cagctcgcgg caatctctgt aattcgcccc
    15121 ggcgtgcgct gccagcaggt tgacgatgcc gcgcgccgcg tgattaccga ggccggttat
    15181 ggcaagtttt tcggccataa caccggtcat gctatcggta ttgaagtcca tgaagaaccg
    15241 cgtttttcac cgcgggacac cacgacgcta cagccaggta tgttactgac cgtggagcca
    15301 gggatttatt tgccagggca agggggcgtg cgcatcgaag atgtcgtgct ggtcaccccg
    15361 caaggcgcag aagtgctcta cgccgtgccg aaaacagtgt tgctcacggg agaggcataa
    15421 tggatttatc gctcttaaaa gcgttgagcg aggcagatgc gatcgcctcc tcagaacagg
    15481 aagtacggca gatcctgcgg gaagaagcgg atcgcctgca aaaagaagtg cgatttgacg
    15541 gcctgggatc ggtgctgatc cgactcaatg agtcgacagg tccgaaggtg atgatctgtg
    15601 cgcatatgga cgaagtggga tttatggtgc gcagcatctc ccgcgaaggg gcgatcgatg
    15661 tgttgccggt tggcaatgta cgcatggctg cccgccagct tcagtcggtg cgtatcacta
    15721 cccgtgaaga gtgcaaaatt ccaggcctgc ttgacagcga ccggcagggg aatgacgtca
    15781 gcgccatgcg cgtggacatt ggcgcgcgct cctgtgacga agtgatgcag gcaggaattc
    15841 gtccaggcga tcgcgtcacc ttcgatacca cttttcaggt tctccctcac cagcgggtga
    15901 tggggaaagc ctttgatgac cgcctcggtt gctatctgct ggtgacgtta ctgcgtgagc
    15961 tgcacgacgc cgaactgcct gcggaagtgt ggctggtggc aagttccagc gaagaggtgg
    16021 gattacgcgg cgggcaaact gccacccgcg cggtgtcgcc ggatgtcgcc attgtgcttg
    16081 atactgcctg ctgggcgaaa aactttgatt atggcgcagc taaccatcgc cagattggta
    16141 acgggccgat gctggtgtta agcgacaagt cactgattgc gccgccaaaa cttaccgcct
    16201 ggatcgaaac cgtggcggca gaaattggcg taccgttgca gtcagatatg ttcagcaatg
    16261 gcggcacgga cggtggggcg gtgcacttaa ccggcactgg cgtacccaca gtggtgatgg
    16321 ggcctgcaac ccgccacgga cattgcgccg catcgattgc tgactgccgt gacattttgc
    16381 agatggagca acttttatct gcccttattc aacgtcttac gcgtgagacg gttgttcaac
    16441 tgacggattt cagatgatct cctgattaac ccggagcggt tatgttaacg attcaatttc
    16501 tctgtccttt gcctaacggt ctacatgctc gtccggcgtg ggaacttaaa gagcagtgca
    16561 gccagtggca aagcgaaatc acttttatta accatcgcca gaacgcaaag gccgatgcga
    16621 aaagctcgct ggcgctgatt ggcactggca ctttatttaa tgacagttgc agcctgaaca
    16681 ttagcggtag cgatgaagag caggcgcggc gagtgctgga agagtatatc caggtgcgct
    16741 ttatcgacag cgatagcgtc cagccgacgc tggcagaact gacggcgcat ccgctgccgc
    16801 gttcattaag ccgcctgaac ccggatttac tgtacggcaa cgtgctggca agcggcgttg
    16861 gcgtgggtac actgaccctg ttacagagcg acagcctcga cagttatcgg gtgatccctg
    16921 cgagtgcgca agattccacc ctactggagc acagtctggc aacgcttgcc gagcaactga
    16981 accagcaatt gcgtgagcgt gacggcgaaa gcaaaactat cctcagcgcc catttgtcgc
    17041 tgattcagga tgatgaattt gcaggcaata tccgtcacct gatggcagaa cagcatcagg
    17101 ggctgggggc ggcgatcatc agcaatatgg agcaggtttg tgccaaactt tctgcttctg
    17161 ccagcgatta tctgcgcgag cgggtcagcg acattcgcga tatcagcgaa cagttgctgc
    17221 atatcacctg gccggaacta aaaccgcgca ataatctggt gcttgaaaaa ctgaccattc
    17281 tggtggctga agatttaacc ccaagccagt ttttgagcct cgatttaaaa aatcttgcgg
    17341 gcatgattct ggagaaaacc gggcgcacct cgcataccct gatcctggcc cgcgcttcgg
    17401 cgatcccggt actgagcgga ctgccgctgg atgcgattgc ccgttatgcc gggcaacctg
    17461 cagtgcttga cgcccagtgc ggcgtgctgg cgattaaccc gaatgacgcg gtgagcggtt
    17521 attatcaggt cgcgcagacg ctggcggata aacgccaaaa acaacaggcg caagctgccg
    17581 cgcagctggc ctattcccgt gataacaaac gtattgatat tgcggcgaat attggcaccg
    17641 ctctggaagc cccaggcgcg tttgctaacg gcgcggaagg cgttgggctg tttcgtaccg
    17701 aaatgctcta tatggatcgc gacagcgcgc cggacgagca ggaacagttt gaagcgtacc
    17761 agcaggtgct attggcggcg ggcgacaagc cgattatctt ccgcacgatg gacatcggtg
    17821 gcgataaaag cattccttac ctcaacattc cccaggaaga gaacccgttc ctcggctatc
    17881 gcgcggtacg tatttacccg gaatttgctg gcctgttccg cactcaattg cgggcgattt
    17941 tgcgcgctgc cagtttcggc aacgcccagt tgatgatccc aatggtccac agtctcgatc
    18001 agatcttatg ggtgaagggt gagatccaaa aagcgatcgt tgagcttaag cgcgatggcc
    18061 tgcgtcatgc agagacgatt acgctgggta tcatggtgga agtgccgtcg gtgtgctaca
    18121 tcatcgacca cttctgcgat gaggtcgatt tcttcagtat cggttctaac gatatgaccc
    18181 agtatctgta tgcagtcgat cgtaataacc cgcgcgtatc gccgctgtat aacccgatta
    18241 cgccatcgtt cctgcgcatg ttgcagcaga ttgttaccgc tgcgcatcag cgcggcaaat
    18301 gggtaggtat ttgcggtgaa ctgggcggtg aaagtcgtta tctgccgcta ctgctagggc
    18361 tggggctgga tgagctaagc atgagtagcc cgcgtattcc ggcagtgaaa agccagcttc
    18421 gtcaactgga tagcgaggcg tgtcgggaac tggcgcgtca ggcgtgcgag tgccgcagcg
    18481 cgcaggaaat tgaagcgtta cttaccgcgt ttgcgccgga agaagacgtt cgcccactgc
    18541 tggcactaga gaatatcttt gttgatcagt cctttagtaa taaagagcag gcgatccagt
    18601 tcctgtgcgg taacctcggt gttaacgggc gtactgaaca tccgttcgag ctggaagaag
    18661 atgtctggca gcgggaagag attgttacta ctggcgttgg ttttggcgtg gcgatcccgc
    18721 acaccaaatc acagtggatc cgtcattcca gtatcagcat tgcccggttg gtgaaaccgg
    18781 ttgactggca gtcagaaatg ggcgaagtcg aactggtgat catgctgacg ctgggcgcta
    18841 acgaagggat gaatcatgtg aaagtcttct cgcagctggc gcgtaaactg gtgaataaaa
    18901 acttccgcca gtcgctgttt gccgcgcaag atgcacaaag tattctgacg ctgctggaaa
    18961 cggaattaac cttctgacgt tagccctgaa aacgtgcgct atactctccc ggcgtcagac
    19021 caaactgacg ccggaaaacg cgacaaaaat agtcgctatc cggaaacccg caacgctgcg
    19081 ccacttcatg aatcgacaga tgatattttt gcaaaatcat cctcgctttt gccattcgta
    19141 cccagcgaac atactcgata aaacgcattg ttccatgctg agcaaacagt tttgacagat
    19201 gatttggcgt gatattaaaa aactgcgcca cgctctcgcg ggtgagcagc tgggcataat
    19261 tatcctgaat ccagttacaa atgctgtgat agagaaaatc gccgcgcggt tgcgaattgc
    19321 ccggcggcat attcaccacg ttgcggcaaa gatgcagcaa acttaacacc agtggctgaa
    19381 tgatattctg ctccagcggc gaacggctaa gatgggtgag ggcggtaagc atcgcttcac
    19441 cttcaccgcg ttgcaggctg ggaagctgga tctggcgagc aggatgtaac aacgacgtgg
    19501 tgcggctatc gtaaaacgat aacccaagcc aggtcggtgc gaacaccagg cttaacagca
    19561 gcaccggttt gttgttgacc ggtaaattag ccgcgcgtgc tggaataaac agcatttctc
    19621 cttccaccat acgttgctgc tttgcttcca gcagattgcc gtattcgccg cgtagcacga
    19681 tatccagacg gggaaaatca atacataaac tgcccactgg caatgaggta ggctggctgg
    19741 caaaccagac gcgccccagt agttgcgggt taagcaccag gccgctgaac agatcggcga
    19801 aaaattgttg atcggcgggc aagcctggtg ctttcatgaa agttcccgat taaagatgca
    19861 ttaactggcg gaattcttta actttgctgc ggctgaccgg cacttcaaaa tccagatctt
    19921 tcagccgcag aatataggtg ttattaaacc acggttcgat ctcgcggatt ttgttcagat
    19981 tgacacaaaa tgagcgatgg cagcggaaaa aatgcgacgg cggcagcttg ctgcaaaact
    20041 cggtaatgtt catcggcatt acgtaggatt cacgccgcgt atagacaaag gtcattttct
    20101 catgcgcttc ggcgtaatag atatcgttaa tgggcgtgac gatgattcgc tcatctttca
    20161 ccagattaat ggtgtcattc tcacgcgtta ccgtcgcggc aggcgtactg ctggtctgct
    20221 gttgttgcca ggctgcttcc agtttttgca gcatcccggt aatgcgtgac tcctgatacg
    20281 gtttgagaat gtagtcaaac gcctccagtt caaacgcttc taccgcatgt tctttccacg
    20341 cggtgataaa cacaataaac ggtttatggg cgaactggct gatgttttgt gccagcaaca
    20401 caccatccag cgacggaata ttgatatcca gaaaaatggc gtcgacgcgg ttatgctgca
    20461 aaaacttcaa cacgtccaga ccgtcgtcaa aggtgccgac aatctccatc tggctgtgct
    20521 ctttaattag ccagctcagt tcctgttgtg ccaggaattc gtcttcaaca atgatgactt
    20581 tcacaatatc actccggctc aaagcaataa ggtagcctgt gaggcgactg gggtgcgttg
    20641 gttaggaatg taaaacgcaa tttccgttcc tggttccagg cggcggatat gcagcccctc
    20701 gccatacaat aacttcacgc gatgatggac attcagcagg ccaattttat tgcccggcat
    20761 ttcgttcgct tcgacccgct caatcacctt tggatcgatg ccgtgcccgg tatctcgcac
    20821 cgcaatgcgt acccgattac cgcactcagc aacgctgatg gtgacaacgc ctttaccttt
    20881 gcaaggctgg ataccgtgga caatggcgtt ttccaccaac ggctggatca gcaggctggg
    20941 aatgcagcaa ttcacctctt catcaatatc atagataacc gtcagcttgt cgccaaagcg
    21001 ggcctgctca atggcaatat agtctttaat ttgatacagc tcttttttga tatcgatttg
    21061 ctcatcgtct tttaattcaa tgttatagcg cagatagcgc gacaaattaa agatcagctg
    21121 gcgagcagta tccggattca gacggattga cgatgaaata gcgttcaaag cgttaaacag
    21181 gaaatgggga ttaattttgc tttgcagggc gcgaagctct gccttatttg ccatttcacg
    21241 tagctgctcg gcgcgtgaca cctcaagttg cgtggagatg atttgtgaca gaccgaccgc
    21301 catttcctgt aatgacgagg ttatctgatg cgcgtggcag tagtaaattt tcagcgttcc
    21361 ggtcacaacg cctttctccc acaatgggat caccagcatg gaatgaatct ctggtgtgcg
    21421 gtgggcttca tcattgtttt taatgatgat tttcccgtaa ttcatcgcct gacgggtagt
    21481 cgggctaatg aagtcatcgc cattctgata gttatgttca cccacgccaa cgtaggccag
    21541 cacatgatcg gtattagtca tcgccaccgc atcggcgtga atgtcatcgc ggataatttc
    21601 gcagaccttg cgtaatgact cgctattgac atggcgaaac agcggcagcg ttttgttggc
    21661 gatatccagc gccagcttgg cctgccgcgc ggcgctggcc tctttttctc cctcaacact
    21721 ttgtaccagc aacacaataa agccgataca gacgctgccg aggatcattg gaatcccgat
    21781 tttagagacg atatcgatcc ccaatgcggt agttggtgcc cagacaatca ccagaatcat
    21841 ggtcaacgtt tcacacaaca tgccgccgag aataccgacg cgccagcgct gtgctttggg
    21901 gattttcagg ttgatccagc ccgatataca acccgccaga atgctggtga taaagcaggg
    21961 gatcgccgtc acgccgccga tgtcaattaa ataacggtga atacccgcaa tcacgccggt
    22021 aatgatgccc acccacgggc caaacagaat cccgcctgac atcaccgcga taatacgtac
    22081 gttcaccagc gagccttcta cgggaacgcc ggaccaggtg ctgaacaggg cgaacagcga
    22141 gaaaatggcg gtaacggcga gcaactcttt tggggagtgc gccgacttgt gcaacagttc
    22201 gcgaaacagg cggatacgga tgagaaagaa caggcagata agcattaacg ctgcccgatc
    22261 gaagaccgcc agcagcatgt tgaatatttc gtgcacgggt gactctgaat aaggattaaa
    22321 cctctatgat aggtaacctg aaggctgatg accagcaggc cttttttgag gaaaaaccac
    22381 aattttaaaa ggggattgaa agataaatgg tgtattagca atgcatctct tgataatgca
    22441 catcttgttg gtaaatttat tgaa
//
LOCUS       DAHHTG010000073        22419 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.073, whole genome shotgun sequence.
ACCESSION   DAHHTG010000073 DAHHTG010000000
VERSION     DAHHTG010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22419)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22419)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22419
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.073"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            complement(<1..211)
                     /gene="cspA"
                     /locus_tag="NBI74_002791"
     CDS             complement(<1..211)
                     /gene="cspA"
                     /locus_tag="NBI74_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone/antiterminator CspA"
                     /protein_id="HCD1933092.1"
                     /translation="MSGKMTGIVKWFNADKGFGFITPDDGSKDVFVHFSAIQNDGYKS
                     LDEGQKVSFTIESGAKGPAAGNVTSL"
     gene            complement(492..782)
                     /locus_tag="NBI74_002792"
     CDS             complement(492..782)
                     /locus_tag="NBI74_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator"
                     /protein_id="HCD1933093.1"
                     /translation="MEYKDPMHELLSSLEQIVFKDETQKITLTHRTTSCTEIEQLRKG
                     TGLKIDDFARVLGVSVAMVKEWESRRVKPSSAELKLMRLIQANPALSKQLME"
     gene            1216..1926
                     /locus_tag="NBI74_002793"
     CDS             1216..1926
                     /locus_tag="NBI74_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3053 domain-containing protein"
                     /protein_id="HCD1933094.1"
                     /translation="MATGKSCSRWFAPLAALLMVVSLSGCFDKEGDQRKAFIDFLQNT
                     VMRSGERLPTLTADQKKQFGPFVSDYAILYGYSQQVNQAMDSGLRPVVDSVNAIRVPQ
                     DYVTQSGPLREMNGSLGVLAQQLQNAKLQADAAHSALKQSDDLKPVFDQAFTKVVTTP
                     ADALQPLIPAAQTFTQQLVMVGDYIAQQGTQVSFVANGIQFPTSQQASEYNKLIAPLP
                     AQHQAFNQAWTTAVTATQ"
     gene            complement(1976..2950)
                     /gene="ghrB"
                     /locus_tag="NBI74_002794"
     CDS             complement(1976..2950)
                     /gene="ghrB"
                     /locus_tag="NBI74_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrB"
                     /protein_id="HCD1933095.1"
                     /translation="MKPSVILYKALPDDLLQRLQEHFTVHQVANLSPQTVEQNAAIFA
                     EAEGLLGSNENVDAALLEKMPKLRATSTISVGYDNFDVDALTARKILLMHTPTVLTET
                     VADTLMALVLSTARRVVEVAERVKAGEWTASIGPDWYGTDVHHKTLGIVGMGRIGMAL
                     AQRAHFGFNMPILYNARRHHKEAEERFNARYCDLDTLLQESDFVCLILPLTDETHHLF
                     GAEQFAKMKSSAIFINAGRGPVVDENALIAALQKGEIHAAGLDVFEQEPLSVDSPLLS
                     MANVVALPHIGSATHETRYGMAACAVDNLINALQGKVEKNCVNPHVAD"
     gene            complement(3054..3713)
                     /locus_tag="NBI74_002795"
     CDS             complement(3054..3713)
                     /locus_tag="NBI74_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family lipoprotein"
                     /protein_id="HCD1933096.1"
                     /translation="MKKRVYLIAAVVSGALAVSGCTTNPYTGEREAGKSAIGAGLGSL
                     VGAGIGALSSSKKDRGKGALIGAAAGAALGGGVGYYMDVQEAKLRDKMRGTGVSVTRS
                     GDNIILNMPNNVTFDSSSATLKPAGANTLTGVAMVLKEYPKTAVNVIGYTDSTGSHDL
                     NMRLSQQRADSVASALITQGVDASRIRTQGLGPANPIASNSTAEGKAQNRRVEITLSP
                     L"
     gene            3866..6199
                     /gene="bisC"
                     /locus_tag="NBI74_002796"
     CDS             3866..6199
                     /gene="bisC"
                     /locus_tag="NBI74_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418007.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin sulfoxide reductase"
                     /protein_id="HCD1933097.1"
                     /translation="MANSSSRYSVLTAAHWGPMLVETDGETVFSSRGALATGMENSLQ
                     SAVRDQVHSNTRVRFPMVRKGFLASPENPQGIRGQDEFVRVSWDEALELIHHQHKRIR
                     EAYGPASIFAGSYGWRSNGVLHKASTLLQRYMALAGGYTGHLGDYSTGAAQAIMPYVV
                     GGSEVYQQQTSWPLVLEHSDVVVLWSANPLNTLKIAWNASDEQGLSYFSALRDSGKKL
                     ICIDPMRSETVDFFGDKMEWVAPHMGTDVALMLGIAHTLVENGWHDEAFLARCTTGYA
                     VFASYLLGESDGIAKNAEWAAEICGVGAAKIRELAAIFHQNTTMLMAGWGMQRQQFGE
                     QKHWMIVTLAAMLGQIGTPGGGFGLSYHFANGGNPTRRAAVLSSMQGSLPGGTDAVDK
                     IPVARIVEALENPGGAYQHNGMDRHFPDIRFIWWAGGANFTHHQDTNRLIRAWQKPEL
                     VVISECFWTAAAKHADIVLPATTSFERNDLTMTGDYSNQHLVPMKQVVPPRDEARNDF
                     DVFAELSERWEKGGYARFTEGKSELQWLETFYNVARQRGASQQVELPPFAEFWQANQL
                     IEMPENPDSERFIRFADFRRDPQAHPLKTASGKIEIFSQRIADYAYPDCPGHPMWLAP
                     DEWQGNAEPEQLQVLSAHPAHRLHSQLNYSSLRELYAVANREPVTIHPDDAQTRGIQD
                     GDTVRLWNSRGQILAGAVISEGIKPGVICIHEGAWPDLDLTADGICKNGAVNVLTKDL
                     PSSRLGNGCAGNTALAWLEKYNGPELTVTAFEPPASS"
     gene            complement(6168..6608)
                     /locus_tag="NBI74_002797"
     CDS             complement(6168..6608)
                     /locus_tag="NBI74_002797"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HCD1933098.1"
                     /translation="MIREAQRSELPALLELWLESTTWGHPFIKSSYWRDCIPLVRDAY
                     LANAQNWVWEEDGKLLGFVSIMEGRFLAAMFVAPKAVRRGIGKALMQYVQQRHPHLML
                     EVYQKNQPAIDFYHAQGFHIVDCAWQDETQLPTWIMSWPVVQTL"
     gene            complement(6605..7168)
                     /gene="tag"
                     /locus_tag="NBI74_002798"
     CDS             complement(6605..7168)
                     /gene="tag"
                     /locus_tag="NBI74_002798"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HCD1933099.1"
                     /translation="MERCGWVSQDPLYIAYHDNEWGVPETDSKKLFEMICLEGQQAGL
                     SWITVLKKRENYRACFHQFDPVKVAAMQEEDVERLVQDAGIIRHRGKIQAIIGNARAY
                     LQMEQNGEPFADFVWSFVNHQPQVTQATTLSEIPTSTPASDALSKALKKRGFKFVGTT
                     ICYSFMQACGLVNDHVVGCCCHPGNKP"
     gene            7326..8024
                     /locus_tag="NBI74_002799"
     CDS             7326..8024
                     /locus_tag="NBI74_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312460.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HCD1933100.1"
                     /translation="MIIKKSGGRWQLSLLASVVISAFFLNTAYAWQQEYIVDTQPGHS
                     TERYTWDSDHQPDYNDILSQRIQSSQRALGLEVNLAEETPVDVTSSMSMGWNFPLYEQ
                     VTTGPVAALHYDGTTTSMYNEFGDSTTTLADPLWHASVSTLGWRVDSRLGDLRPWAQI
                     SYNQQFGENIWKAQSGLSRMTATNQNGNWLDVTVGADMLLNQNIAAYAALSQAENTTN
                     NSDYLYTMGVSARF"
     gene            8253..9461
                     /locus_tag="NBI74_002800"
     CDS             8253..9461
                     /locus_tag="NBI74_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCD1933101.1"
                     /translation="MTPSNYQRTRWLTLIGTIITQFALGSVYTWSLFNGALSAKLGAP
                     VSQVAFSFGLLSLGLAISSSVAGKLQERFGVKRVTMASGILLGLGFFLTAHSNNLMML
                     WLSAGVLVGLADGAGYLLTLSNCVKWFPERKGLISAFAIGSYGLGSLGFKFIDTHLLE
                     TVGLEKTFVIWGAIVLVMIVFGATLMKDAPKQEVKTSNGVVEKDYTLAESMRKPQYWM
                     LAVMFLTACMSGLYVIGVAKDIAQSLAHLDAISAANAVTVISIANLSGRLVLGILSDK
                     IARIRVITIGQVIALVGMAALLFAPLNAVTFFAAIACVAFNFGGTITVFPSLVSEFFG
                     LNNLAKNYGVIYLGFGIGSICGSIIASLFGGFYVTFYVIFALLILSLALSTTIRQPEQ
                     KMLREAHGSL"
     gene            9785..11476
                     /gene="eptB"
                     /locus_tag="NBI74_002801"
     CDS             9785..11476
                     /gene="eptB"
                     /locus_tag="NBI74_002801"
                     /EC_number="2.7.8.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418002.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid A phosphoethanolamine
                     7''-transferase"
                     /protein_id="HCD1933102.1"
                     /translation="MRYIKSITQQKLSFLLAIYIGLFMNGAVFYRRFGSYAHDFTVWK
                     GISAVVELAATVLVTFFLLRLLSLFGRRSWRILASLVVLFSAGASYYMTFLNVVIGYG
                     IIASVMTTDIDLSKEVVGLNFILWLIAVSALPLILIWNNRCRYTLLRQLRTPGQRIRS
                     LAVVVLAGIMVWAPIRLLDIQQKKVERATGVDLPSYGGVVANSYLPSNWLSALGLYAW
                     ARVDESSDNNSLLNPAKKFTYQAPQNVDDTYVVFIIGETTRWDHMGIFGYERNTTPKL
                     AQEKNLAAFRGYSCDTATKLSLRCMFVRQGGAEDNPQRTLKEQNIFAVLKQLGFSSDL
                     YAMQSEMWFYSNTMADNIAYREQIGAEPRNRGKPVDDMLLVDEMQQSLGRNPDGKHLI
                     ILHTKGSHFNYTQRYPRSFAQWKPECIGVDSGCTKAQMINSYDNSVTYVDHFISSVID
                     QVRDKKAIVFYAADHGESINEREHLHGTPRELAPPEQFRVPMMVWMSDKYLENPVNAQ
                     AFAQLKKEADMKVPRRHVELYDTIMGCLGYTSPDGGINENNNWCHIPQAREAAAN"
     gene            11568..11644
                     /locus_tag="NBI74_002802"
     tRNA            11568..11644
                     /locus_tag="NBI74_002802"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11602..11604,aa:Pro,seq:cgg)
     gene            12388..13995
                     /gene="dppA"
                     /locus_tag="NBI74_002803"
     CDS             12388..13995
                     /gene="dppA"
                     /locus_tag="NBI74_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter substrate-binding
                     protein DppA"
                     /protein_id="HCD1933103.1"
                     /translation="MRISLKKSGMLKLGLSLVAMTVAASVQAKTLVYCSEGSPEGFNP
                     QLFTSGTTYDASSVPLYNRLVEFKIGTTEVIPGLAEKWEVSEDGKTYTFHLRKGVKWH
                     DNKEFKPTRELNADDVVFSFDRQKNAQNPYHKVSGGSYEYFEGMGLPELISEVKKVDD
                     NTVQFVLTRPEAPFLADLAMDFASILSKEYADAMMKAGTPEKLDLNPIGTGPFQLQQY
                     QKDSRIRYKAFDGYWGTKPKIDTLVFSITPDASVRYAKLQKNECQVMPYPNPADIARM
                     KQDKSINLMEMPGLNVGYLSYNVQKKPLDDVKVRQALTYAVNKDAIIKAVYQGAGVSA
                     KNLIPPTMWGYNDDVQDYTYDPEKAKALLKEAGLEKGFSIDLWAMPVQRPYNPNARRM
                     AEMIQADWAKVGVQAKIVTYEWGEYLKRAKDGEHQTVMMGWTGDNGDPDNFFATLFSC
                     AASEQGSNYSKWCYKPFEDLIQPARATDDHNKRVELYKQAQVVMHDQAPALIIAHSTV
                     FEPVRKEVKGYVVDPLGKHHFENVSIE"
     gene            14302..15321
                     /gene="dppB"
                     /locus_tag="NBI74_002804"
     CDS             14302..15321
                     /gene="dppB"
                     /locus_tag="NBI74_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppB"
                     /protein_id="HCD1933104.1"
                     /translation="MLQFILRRLGLVIPTFIGITLLTFAFVHMIPGDPVMIMAGERGI
                     SPERHAQLLAELGLDKPMWQQYLHYIWGVMHGDLGISMKSRIPVWEEFVPRFQATLEL
                     GVCAMIFATAVGIPVGVLAAVKRGSIFDHTAVGLALTGYSMPIFWWGMMLIMLVSVHW
                     NLTPVSGRVSDMVFLDDSNPLTGFMLIDTAIWGEDGNFIDAVAHMILPAIVLGTIPLA
                     VIVRMTRSSMLEVLGEDYIRTARAKGLTRMRVIIVHALRNAMLPVVTVIGLQVGTLLA
                     GAILTETIFSWPGLGRWLIDALQRRDYPVVQGGVLLVATMIILVNLLVDLLYGVVNPR
                     IRHKK"
     gene            15331..16233
                     /gene="dppC"
                     /locus_tag="NBI74_002805"
     CDS             15331..16233
                     /gene="dppC"
                     /locus_tag="NBI74_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010426221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppC"
                     /protein_id="HCD1933105.1"
                     /translation="MSQVTENKVISAPVPMTPLQEFWHYFKRNKGAVVGLVYVVIVLF
                     IAIFANWIAPYNPAEQFRDALLAPPAWQEGGSMAHLLGTDDVGRDVLSRLMYGARLSL
                     LVGCLVVVLSLIMGVILGLIAGYFGGLVDNIIMRVVDIMLALPSLLLALVLVAIFGPS
                     IGNAALALTFVALPHYVRLTRAAVLVEVNRDYVTASRVAGAGAMRQMFINIFPNCLAP
                     LIVQASLGFSNAILDMAALGFLGMGAQPPTPEWGTMLSDVLQFAQSAWWVVTFPGLAI
                     LLTVLAFNLMGDGLRDALDPKLKQ"
     gene            16244..17227
                     /gene="dppD"
                     /locus_tag="NBI74_002806"
     CDS             16244..17227
                     /gene="dppD"
                     /locus_tag="NBI74_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HCD1933106.1"
                     /translation="MALLNVDKLSVHFGDESAPFRAVDRISYSVKQGEVVGIVGESGS
                     GKSVSSLAIMGLIDYPGRVMAEKLEFNGQDLQRISEKERRNLVGAEVAMIFQDPMTSL
                     NPCYTVGFQIMEAIKVHQGGNKSTRRQRAIDLLNLVGIPDPASRLDVYPHQLSGGMSQ
                     RVMIAMAIACRPKLLIADEPTTALDVTIQAQIIELLLELQQKENMALVLITHDLALVA
                     EAAHKIIVMYAGQVVETGDAHAIFHAPRHPYTQALLRALPEFAQDKERLASLPGVVPG
                     KYDRPNGCLLNPRCPYATDKCRAEEPALNMLADGRQSKCHYPLDDAGRPTL"
     gene            17224..18237
                     /gene="dppF"
                     /locus_tag="NBI74_002807"
     CDS             17224..18237
                     /gene="dppF"
                     /locus_tag="NBI74_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP binding subunit
                     DppF"
                     /protein_id="HCD1933107.1"
                     /translation="MSTHEATPQQPLLQAIDLKKHYPVKKGMFAPERLVKALDGVSFN
                     LERGKTLAVVGESGCGKSTLGRLLTMIETPTSGELYYQGQDLLKHDPQAQKLRRQKIQ
                     IVFQNPYGSLNPRKKVGQILEEPLLINTSLSKVQRREKALSMMAKVGLKTEHYDRYPH
                     MFSGGQRQRIAIARGLMLDPDVVIADEPVSALDVSVRAQVLNLMMDLQQELGLSYVFI
                     SHDLSVVEHIADEVMVMYLGRCVEKGTKDQIFNNPRHPYTQALLSATPRLNPDDRRER
                     IKLTGELPSPLNPPPGCAFNARCRRRFGPCTQLQPQLKDYGGQLVACFAVDQDENPQK
                     PAV"
     gene            18462..18785
                     /locus_tag="NBI74_002808"
     CDS             18462..18785
                     /locus_tag="NBI74_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000563102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCD1933108.1"
                     /translation="MICSGPQNLAQELKAIGDQLQELQKKLIQGPNISQPRPLLTIET
                     PRHLGEQLNARRKELGIDLYTLELQTGISTSTLKRLFKDPEQVKFGSVFAVANVLGVK
                     LCIGE"
     gene            18770..19081
                     /locus_tag="NBI74_002809"
     CDS             18770..19081
                     /locus_tag="NBI74_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HipA N-terminal domain-containing protein"
                     /protein_id="HCD1933109.1"
                     /translation="MHRRVKVLLYGQVVGELSQNDSGFLFQYAPDYHGPAISISLPVA
                     QRQFPSEMLHPYFASLAPEGWLRQRYSQIQHRDENDLLGMLIDNGKNLLGAIQILPWE
                     E"
     gene            19081..20088
                     /locus_tag="NBI74_002810"
     CDS             19081..20088
                     /locus_tag="NBI74_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001262465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HipA domain-containing protein"
                     /protein_id="HCD1933110.1"
                     /translation="MANCRILLTPLNERDEQRGYSTQGLKRLSGTAKLTPRLGFTRTQ
                     FVQELPRQQKGMSISGYQPKLQLVLDEGEFRVVDHQGNFILKPSPADFPGLAENEHAT
                     MTLMSRLGFDVPVHGLLSFAPQSEEELEYAFVIRRYDRDNKGLPVHQEQLDGAMQIMD
                     KYGKTGNDNEQYVSYETLARFLVAHVNDNIAFKIDLFRRIVYAWLLGNNDMHLRNFGL
                     VYSDGLTPALAPVYDFVSVAPYPEYFHSNYLALPLLTREEGGRELAPGFHSDYGEYIG
                     QDFLLLGESMGLAPRLLEKLFQDIRKENAIVMETYEQSFMTQDHIQAVLQCYRHRLGL
                     L"
     gene            complement(20105..21376)
                     /locus_tag="NBI74_002811"
     CDS             complement(20105..21376)
                     /locus_tag="NBI74_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HCD1933111.1"
                     /translation="MQHNTLPKHDQKLPFTRYDFGWVLLCIGMAIGAGTVLMPVQIGL
                     KGIWVFITAAIIAYPATWVVQDIYLKTLSESDSCNDYTDIISHYLGKNWGIFLGVIYF
                     LMIIHGIFIYSLSVVFDSASYLKTFGLTDADLSQSLFYKVAIFAVLVAIASGGERLLF
                     KISGPMVVVKVGIIVVFGFAMIPHWNFANITAFPQASDFFRDVLLTIPFCFFSAVFIQ
                     VLNPMNIAYRKREADKVLATRLALRTHRISYVTLIAVILFFAFSFTFSISHEEAVSAF
                     EQNISALALAAQVIPGHIIHITSTVLNIFAVLTAFFGIYLGFHEAIKGIILNLLSRII
                     DTRKINSRVLTLAICTFIVMTLTIWVSFRVSVLVFFQLGSPLYGIVSCLIPFFLISKV
                     SQLKKLRGLKTWLILFYGILLCFSPLLKLIE"
     gene            21852..21959
                     /locus_tag="NBI74_002812"
     CDS             21852..21959
                     /locus_tag="NBI74_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HCD1933112.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
     gene            22335..>22419
                     /locus_tag="NBI74_002813"
     CDS             22335..>22419
                     /locus_tag="NBI74_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HCD1933113.1"
                     /translation="MTLAELGMAFWHDLAVPVITGILASMIV"
BASE COUNT         5387 a         5472 c         5905 g         5655 t
ORIGIN      
        1 acaggctggt tacgttacca gctgccgggc ctttagcgcc gctttcgatg gtgaaggaca
       61 ctttctgacc ttcgtccaga gatttgtaac catcgttctg gatagcagag aagtgtacga
      121 acacgtcttt agagccatcg tcaggagtga tgaagccgaa gcctttgtca gcgttgaacc
      181 attttacgat accagtcatt ttaccggaca tagtgtatta cctttaataa ttaattgtgc
      241 ctttcggcga tatggcgtgc tttacagatt ttgaagcgtt aaaggaatgt gcactacgag
      301 gggtatcaac gataactctt gaagggactt gccttactac actgctttaa tggtctgtac
      361 gtcaaaccgt tgatgtgcat taagccacgc attggtgggt gatgcaacaa ttatttttca
      421 tatttatgat taatcggttg gaaaacggtc ctgttatcag gaccgtaaac agaaataaag
      481 tggataaaag cctattccat caactgctta cttaatgccg ggttggcttg aatcaaacgc
      541 atcaatttta gttcggcact tgaaggcttc acgcgtctgg attcccattc ctttaccatg
      601 gcgactgata cgcccaaaac ccgggcgaaa tcatcgattt ttaatcctgt cccttttcgt
      661 aactgctcaa tttcggtaca ggacgttgtt ctgtgcgtca gggtaatttt ctgcgtttca
      721 tctttaaaaa caatctgttc caggctgctc aacagctcat gcattggatc tttatattcc
      781 attgaaaact cctcaaatca cactgcggga tcgtgaattg catcgaagct cattaagaat
      841 agtcgggaaa aacagacaca gcggggtgta gtcagggcat actctgacta tgattcgatt
      901 cagccagtca tttagcgtga cttatagcca cctaacgatc tgataaatgc cgatatatct
      961 cttaccactc ccggtttgta aagatttcga aaatctaacg ttctgcgaag gcgtaaatct
     1021 ctggctacaa aaaaggcaac ggcaatatca ttttttaaca atgaaacaac aaattaacct
     1081 tgtttcattt tctccatgat tttaccagga agattatgta gaaagggtat cctgcaagcc
     1141 tgtcctggac tatccttgtc acgtcagaca cgcgtgtgtc gttgtgcgct ttttttgggt
     1201 gaaaggagta agaaaatggc gacaggaaag tcctgctctc gctggtttgc gcctcttgcg
     1261 gcgttattaa tggtagttag cctgagtggg tgttttgata aagaaggcga tcagcgtaaa
     1321 gcgtttatcg acttcctgca gaatacagtg atgcgtagcg gtgaacgtct accaaccctg
     1381 actgccgatc agaaaaaaca gtttggtcct tttgtctctg attacgcgat tctgtatggt
     1441 tattctcagc aggtgaatca ggcgatggat tccggtctgc gcccggttgt agacagcgtt
     1501 aacgcaattc gcgtaccgca ggattacgtt acgcaaagcg gtccgctgcg tgaaatgaac
     1561 ggttcgctgg gtgtgctggc gcaacagctg caaaatgcga agttgcaggc cgatgctgcg
     1621 cactctgcgt taaaacagag tgatgacctg aaaccggtct ttgatcaggc attcaccaaa
     1681 gtggtaacga cgccagctga tgcattgcaa ccgttgattc cggcggcaca aactttcacg
     1741 caacaactgg tgatggttgg agactacatt gctcagcaag gtactcaggt gagctttgtc
     1801 gcaaatggca tccagttccc gacttcacag caggcaagtg aatataacaa actgattgcg
     1861 ccattaccgg cacaacatca ggcgtttaat caggcctgga ctacagcagt tactgcaacc
     1921 caataaagag taaaagcccg agcgattatc gggctttact cctcgcagtc gcggcttagt
     1981 ccgcgacgtg cggattcaca cagttcttct caacctttcc ttgtaacgca ttaatcaaat
     2041 tatccacggc acaggcggcc atgccataac gcgtctcatg ggtggcagat ccaatatgcg
     2101 gtaatgcgac gacgttggcc attgagagca acggcgaatc tacggacagt ggctcttgtt
     2161 cgaagacatc cagcccggcg gcgtgaattt cccctttctg caatgcagca atcagtgcat
     2221 tttcatcaac caccgggcca cgtccggcat taatgaaaat ggcggaggat ttcattttgg
     2281 cgaattgttc tgcgccaaac agatgatgcg tctcatccgt taacggcagg atcaggcaaa
     2341 cgaaatctga ctcttgtaac agagtatcca aatcgcagta gcgggcgttg aagcgttctt
     2401 ctgcttcttt atggtggcga cgcgcgttat agaggatggg catgttgaag ccaaagtgcg
     2461 cacgttgtgc cagcgccatg ccgatccgtc ccatcccgac aatgcccagt gttttatggt
     2521 gaacgtcagt gccgtaccag tccgggccta tactcgcggt ccattcgcct gcttttaccc
     2581 gttctgctac ctccacaacc cgacgagcgg tagacaacac cagcgccatc agcgtatcgg
     2641 cgacggtttc tgttaatacg gttggtgtgt gcatcagcag aatttttcgg gcggtaagcg
     2701 catcgacatc aaaattgtca tagccgacgg agatcgttga tgtggcacgc agtttcggca
     2761 ttttttccag caatgcggca tcaacattct catttgaacc cagcaagcct tcagcttcgg
     2821 caaaaattgc tgcattttgt tcgacggttt gtgggctgag gtttgctacc tggtgaacgg
     2881 tgaaatgttc ttgcaggcgt tgcagtaaat catcaggtaa ggctttgtag aggataacgg
     2941 acggcttcat gcttctctcc attcactgat aaggcgatca ccttagcctg aaatatgatg
     3001 aaggaaaact gaagaatgcc aggtgatttc tgcatcacct gacatgaaag ggattacagt
     3061 gggcttaagg taatttctac acggcggttt tgcgccttac cttctgcggt gctgttgctg
     3121 gcgattgggt tagccgggcc aaggccctga gtacggatgc ggctggcgtc cacgccctgg
     3181 gtgatcaacg cgctggcaac ggaatccgca cgctgctggg agagacgcat gttcaggtcg
     3241 tgactccccg tgctgtcggt ataaccaatc acgttaaccg ccgttttcgg atactctttc
     3301 agtaccattg ccacgccggt cagggtgtta gcgcccgccg gtttcagggt tgcgctgctg
     3361 ctgtcgaagg tcacattgtt cggcatattg aggataatgt tatccccgct gcgggttacg
     3421 ctaacaccag tgccgcgcat tttgtcacgc agcttcgctt cctgcacatc catgtaataa
     3481 ccaacgccgc cgcccagagc tgcgcctgct gctgcgccaa tgagcgcgcc tttaccgcga
     3541 tctttcttcg aagaagagag cgcaccaata cccgcgccaa cgagagagcc cagacctgcg
     3601 ccgatagcag atttacctgc ttcgcgttcg ccggtgtaag ggttagttgt acagccagat
     3661 accgccagag cgccactcac tacggcggca ataagataaa cacgtttctt cattgttaat
     3721 ccttaataac ctttttattc tttgccacgg gttccgtggc gggagattat gccgcgtgag
     3781 catgaagatt attcctggga atactcggaa atttgtaagt aatatttaac tgctcaatac
     3841 atctaacctt tcaggagtct tcggtttggc caactcatcc tcacgatatt ccgttctgac
     3901 tgccgcccac tgggggccta tgctggttga aaccgacggc gaaaccgtgt ttagctcgcg
     3961 tggcgcgtta gccacaggaa tggaaaactc cttgcagagc gcggttcgcg accaggttca
     4021 cagcaatacg cgggtgcgat ttccaatggt gcgcaaaggc tttcttgcgt caccggaaaa
     4081 tccgcaaggc attcgtgggc aggatgaatt tgttcgcgtg agttgggatg aggcgctgga
     4141 gcttattcac catcaacata aacgcattcg tgaggcttat ggtccggcat cgatttttgc
     4201 tggttcctac ggctggcgtt caaacggcgt gctgcataag gcctcgacat tattacaacg
     4261 ctatatggcg ttggcaggcg gttatactgg gcatctgggg gattattcga ccggcgcggc
     4321 acaggcgatc atgccgtatg tcgtgggtgg cagcgaagtt tatcaacagc aaaccagttg
     4381 gccgctggtg ctggaacata gcgatgtcgt ggtgctgtgg agtgctaacc cactcaatac
     4441 gctgaaaatt gcgtggaatg catccgatga gcaggggctt tcttactttt ccgcactgcg
     4501 tgacagcggg aaaaagctga tctgcattga tccaatgcga tcggaaaccg tcgatttctt
     4561 tggcgataaa atggagtggg tggcaccgca catgggcacc gatgttgcgt tgatgctggg
     4621 gatcgcccat acgctggtgg aaaatggttg gcacgacgaa gcgtttctgg cgcgttgcac
     4681 cacaggttat gctgtcttcg cctcttattt gctgggcgag agtgacggaa tagcgaaaaa
     4741 cgccgaatgg gcggcagaga tttgtggtgt tggcgcagcg aaaatccgcg agctggcggc
     4801 tattttccac caaaatacaa ccatgctgat ggcaggctgg ggaatgcagc gccaacagtt
     4861 tggtgagcaa aaacactgga tgatcgtcac gctggcggca atgttggggc aaatcggcac
     4921 acccggcggc ggttttggtc tttcttacca ttttgccaat ggtggtaacc ccacgcgccg
     4981 cgctgcggtg ctctcttcca tgcagggcag cttgccgggt ggcaccgatg cggtagataa
     5041 aatccctgtc gcccgcattg ttgaagcact ggaaaaccct ggtggcgcat atcaacataa
     5101 cggtatggac cgacatttcc cggatattcg ttttatctgg tgggcgggtg gtgccaactt
     5161 tactcatcat caggatacca atcgcctgat ccgtgcctgg caaaaaccgg agctggtggt
     5221 gatctccgaa tgcttctgga cggctgcggc gaaacacgcg gatatcgttc tgcctgcgac
     5281 cacctctttt gagcgtaatg atctcaccat gaccggtgat tacagtaatc agcatctggt
     5341 gccgatgaag caagtggtgc cgccacgcga tgaagcgcgt aatgattttg atgtttttgc
     5401 cgagttgagt gaacgttggg agaagggcgg ttatgcacgt tttacggaag gaaaaagtga
     5461 gctgcaatgg ctggaaacgt tttataacgt tgcccggcag cgcggggcaa gccagcaggt
     5521 tgaattgccg ccatttgctg agttctggca agccaaccag ttaattgaga tgccggaaaa
     5581 cccggacagt gagcggttta ttcgcttcgc cgattttcgc cgcgatccgc aggcgcatcc
     5641 attaaaaacc gccagcggta agattgaaat cttctcgcag cgtattgccg attacgctta
     5701 tccggattgc ccggggcatc caatgtggct ggcgccggac gaatggcagg gtaatgccga
     5761 accggaacag ttgcaggtac tttctgctca tccggcgcat cgtctgcaca gccagctgaa
     5821 ttacagttct ctgcgcgaat tgtacgcggt ggcaaatcgt gagcctgtca ccattcatcc
     5881 tgacgatgcc cagacgcgcg gcatacaaga tggcgatact gttcggctgt ggaactcacg
     5941 cgggcaaatt cttgccggcg cggtcattag cgagggaatt aaacctggcg tgatttgtat
     6001 tcacgaaggc gcatggccgg atttggattt aaccgctgac ggcatttgta aaaacggcgc
     6061 agtgaacgtg ctgaccaaag atctccccag ctcgcggctg gggaatggct gtgcgggtaa
     6121 tacggcgctg gcatggctgg aaaaatacaa cggtccggaa ctgacagtta cagcgtttga
     6181 accaccggcc agctcataat ccaggtgggt agttgggttt catcctgcca tgcgcaatcg
     6241 acaatgtgaa aaccctgcgc atggtagaaa tctatcgccg gttgattttt ttgataaacc
     6301 tccagcatca gatggggatg gcgctgctgc acatactgca tcagcgcctt accaataccg
     6361 cgcctgacgg ccttcggtgc gacaaacatt gctgccagaa atcggccttc cataatgctg
     6421 acaaaaccga gaagcttacc gtcttcttcc cagacccagt tttgcgcgtt ggcaagatac
     6481 gcatcccgca ccagcggaat gcagtcacgc cagtaactcg attttataaa gggatgcccc
     6541 caggttgtac tttccagcca cagttcgagg agcgcgggga gttctgaacg ttgcgcttcc
     6601 cgaatcatgg tttatttccc ggatggcaac agcagccaac cacatgatca ttcaccagcc
     6661 cacatgcctg cataaaggag taacagattg tggtaccgac aaacttaaaa ccacgttttt
     6721 ttagtgcctt agatagggcg tcggaggcgg gtgtagatgt ggggatttcg ctcaacgttg
     6781 tggcttgtgt cacctgtggt tgatgattga caaacgacca gacaaagtcg gcaaacggtt
     6841 cgccgttctg ttccatttgc aggtacgccc gcgcattacc aataattgcc tgaattttcc
     6901 cacgatggcg gataatcccg gcgtcctgta ccagtctttc gacatcctct tcctgcattg
     6961 cggcaacttt caccggatcg aactgatgaa agcatgcgcg atagttttcg cgttttttga
     7021 ggacggtgat ccacgataac ccagcctgct gcccttccag gcagatcatt tcgaacagtt
     7081 ttttactgtc agtttcaggc acgccccatt cattatcatg gtaggcaata taaagcgggt
     7141 cctgactcac ccagccgcaa cgttccatac tttccctcgc tatgtacccg atcgtaaaaa
     7201 tttcagccgc ttttcttgac gcatcggtga aaaagtcaat atttaataca gggctactat
     7261 gcccgatatc cttcatacaa tgacaataat attgccgaat tcggctcttc cctggagtcg
     7321 tgttaatgat cataaaaaaa agcggtggtc gctggcagct aagcctgctt gcgagcgtgg
     7381 taatcagtgc cttttttctc aacacagctt acgcctggca acaagaatat atcgttgata
     7441 cgcaacccgg acattccaca gagcgttaca cctgggatag cgatcatcaa cctgattaca
     7501 acgatatttt gtcgcaacgt attcaaagta gccaaagggc gctgggactg gaagtcaatc
     7561 tggcggaaga aacccctgtg gatgtgacca gcagtatgag tatgggctgg aattttcctt
     7621 tatatgagca ggttacaacc ggcccggtcg cggcattaca ttacgatggc acaaccacct
     7681 cgatgtataa cgagtttggc gacagtacta ccacgctggc cgatccgtta tggcatgcca
     7741 gcgtgagtac cttaggctgg cgtgttgact cccggcttgg cgatctccga ccctgggcgc
     7801 aaatcagcta taaccagcaa tttggcgaga atatctggaa ggcgcaatca ggcctgagcc
     7861 ggatgacggc gacaaaccag aacggcaact ggctggatgt caccgtaggc gctgatatgt
     7921 tgctcaatca aaatattgct gcctatgctg cgctatctca ggcagaaaat accactaaca
     7981 atagcgacta tctgtatacg atgggggtta gtgccagatt ttaacgttat agatacaatt
     8041 gaaacccata aataacaact aatgtggcga tagtggggac taacttacta aataataaat
     8101 ttggcgaata attgtcgagt cattcattcc tgaactaatg catttcattc cgttctgatg
     8161 gcatttcatg ccgtttttcc ccaggcataa agcgcacttc gttatggttg tcagcagaga
     8221 tttttccttt ttattactgc aggaatactg ccatgacacc ttcaaattat cagcgtactc
     8281 gctggctgac actcatcggt actatcatta cccagtttgc tctggggtcg gtttatacct
     8341 ggagtctgtt taacggtgcg ctttctgcca agctgggggc tcctgtaagc caggttgctt
     8401 tctctttcgg cttgttaagt ctggggctgg caatttcgtc ttctgttgcg ggcaaattgc
     8461 aggaacgttt tggtgttaaa cgcgtcacta tggcttccgg cattttgctg ggattaggtt
     8521 tcttcctgac agcacattcc aacaacctga tgatgctgtg gttaagcgcc ggtgtgttgg
     8581 ttggtctggc ggatggtgcg ggttacctgt tgacgctctc taactgcgtg aagtggtttc
     8641 cggagcgtaa gggacttatc tctgcttttg ctatcggttc ttatggtctg ggcagcctgg
     8701 gttttaaatt tatcgacacg cacctgctcg aaacggtggg tctggaaaaa acctttgtta
     8761 tttggggcgc gattgtactg gtgatgattg tctttggcgc aacgttaatg aaagatgcgc
     8821 cgaagcagga agtgaaaacc agcaatggtg tggtggagaa ggactacacc ctggcagagt
     8881 cgatgcgtaa accgcagtac tggatgttag cggttatgtt cctgactgcg tgcatgagtg
     8941 gtctgtatgt gattggtgta gcgaaagata tcgctcaaag tctggcgcat cttgatgcaa
     9001 tttccgcagc caatgctgtg acggttattt ccatcgccaa cctttctggt cgtctggtgc
     9061 tgggcattct gtctgacaaa atcgcccgta tccgtgttat caccattggt caggtgattg
     9121 cgctggtggg tatggcggcc ctgctgtttg caccattgaa tgcagtgacg ttctttgcag
     9181 cgattgcctg tgtggcattt aactttggtg gcacgattac ggtgttcccg tcactggtca
     9241 gtgagttctt cggcctcaat aacctggcga aaaactacgg cgtcatttac ctcggtttcg
     9301 gtatcggcag catttgtggt tcgattatcg cttcactgtt tggcggattc tatgtgactt
     9361 tctacgtgat tttcgccctg ctgattctgt cactggcgct ttctacgacc attcgccagc
     9421 cagagcagaa aatgttgcgt gaggcgcatg gctcccttta atgctcagcc gtgagctact
     9481 gggcttcagc ttcagtgtgc atcaaacgca tcaacgaaat gtataaacaa taacgcgtaa
     9541 aatttgggcc agttaactgg ccctttttac tgctttttgt aacgctaaag tctcttttca
     9601 aacttgcatt tttgtaaatt tgtgcttcgt gcacactctt tccccacact ttttcccttt
     9661 gctgtggtct acttattcgc gcgtgtagat tttacttatc tgactacctc cgcacttttc
     9721 ccctgcctgg cctgaaaagc cactaagcag ggtgttatca cctgtttgtc cagggtttgt
     9781 ttgcatgaga tacatcaaat cgattacaca gcagaagctg agctttttgc ttgcaatcta
     9841 tattggcctt tttatgaatg gcgcggtttt ttaccgccgc tttggcagct atgcgcacga
     9901 ttttaccgtc tggaaaggca tttctgctgt tgttgaactg gccgccaccg tactggtgac
     9961 cttcttttta ctgcgtctgc tttcgctgtt tggccgccgc agctggcgta ttctggcatc
    10021 gctggtggtg ctcttttctg caggtgccag ctattacatg accttcctta atgtagtcat
    10081 tggttatggc atcatcgcat ccgtcatgac caccgatatc gacctgtcaa aagaagttgt
    10141 tggtctgaac tttattctct ggttaatcgc cgtaagcgcg ttgcctctta tccttatctg
    10201 gaataaccgc tgtcgttaca ccttgctccg acaactgcga accccagggc aacgtattcg
    10261 cagcctggcg gtcgtcgtac tggcaggtat tatggtttgg gcaccgattc gtttgctgga
    10321 tatccagcag aagaaagtgg agagggcgac aggtgttgat ttgccgagtt atggcggcgt
    10381 cgtcgcgaac tcttatctgc catcaaactg gctttcggca ttaggtcttt atgcctgggc
    10441 gcgggtcgat gaatcttccg ataacaattc tctccttaac cctgcgaaga agtttacgta
    10501 ccaggctcca caaaacgttg atgatacata tgtcgttttc attattggtg aaaccacgcg
    10561 ctgggatcat atgggcattt tcggctatga acgcaatacc acaccgaaac tggctcagga
    10621 gaaaaatctg gcggcgttcc gtggttactc ctgtgatact gcaaccaaac tctcactgcg
    10681 ttgcatgttt gtacgtcagg gtggagcaga agataatccg cagcgcacat taaaagaaca
    10741 gaacattttc gcggtattga agcagttagg attcagttct gatctctacg ctatgcaaag
    10801 cgaaatgtgg ttctacagca atacgatggc ggacaacatt gcttatcgtg agcagattgg
    10861 tgcggagcca cgtaaccgtg gcaaaccggt agatgatatg ttgctggtag acgaaatgca
    10921 gcaatcgctg gggcgcaacc cggatggtaa gcatctgatc attctgcata ccaaaggctc
    10981 acactttaac tacacccagc gttacccgcg cagctttgcg cagtggaagc cggaatgtat
    11041 tggtgttgat agcggctgta ccaaagcgca gatgatcaac tcatacgaca actcggtgac
    11101 ctatgtggat cactttattt ccagcgtgat tgatcaggtt cgcgataaga aagcgattgt
    11161 gttctatgca gctgaccacg gtgagtcaat taatgaacgc gagcacctgc acggcacgcc
    11221 gcgtgaactg gcaccgccgg aacagttccg tgtgccgatg atggtctgga tgtcggataa
    11281 atatctggaa aatccggtca atgcgcaggc atttgcgcag ctgaaaaaag aagccgacat
    11341 gaaagtgcca cgccgtcatg tagagctgta cgacaccatc atgggttgtc ttggctatac
    11401 ttcaccggat ggtggaatta acgaaaacaa caactggtgc cacatcccgc aggcaagaga
    11461 ggcggcggct aactaagagg cctgctgact ttctcgccga tcaaaaggca ttttgctatt
    11521 aagggattga cgagggcgta tctgcgcagt aagatgcgcc ccgcattcgg tgattggcgc
    11581 agcctggtag cgcacttcgt tcgggacgaa ggggtcggag gttcgaatcc tctatcaccg
    11641 accaaattcg aaaagcctgc tcaacgagca ggcttttttg catctgtaac tcatgaggaa
    11701 gagaaccccc gggggcgggg aggttcgact cgacgtaggc ctgataagac gcaccagcgt
    11761 cgcatcaggc aaatgtaccg gcccattgtc ggatgcggcg cgaccgcctt atccgatcta
    11821 cacaccacac atttccctgt caaatttgcg ccccatatct cactatccat taacggattt
    11881 gtgacagaaa ccattgtctt tttttatatt tgcttaaatc tttttttgcg ttacttatgg
    11941 ctggcgctgg cattttccgc tgtgcgtttt agcgttcatg gtattaatcg ctcagatatc
    12001 cacctttacc tcagattttt ttcattaaat gcataaaact taatcaccaa atgttgctaa
    12061 atattaagct gattgttctg gttgtgttgg cgtagcacaa atttctctgc tgcaaatagc
    12121 tgtttgaagt ttttttaatc tttgtttgtg gattctcacc gttggtgtaa atcccggttg
    12181 gctgtattga cgttttcaca ttctgttgac agattgtagg tcacgagggg cattttatgg
    12241 aggatccgca ctgttacact gatgttaatt agtacggcat ccccacctca taacgttgac
    12301 ccgaccgggc aaaaaacaaa aaaggtcagg cagcgacaac ccactgcaaa gggttaaaac
    12361 aacaaacatc acaattggag cagaataatg cgtatttcct tgaaaaagtc agggatgctg
    12421 aagcttggtc tcagcctggt ggctatgacc gtcgcagcaa gtgttcaggc taaaactctg
    12481 gtttattgct cagaaggatc tccggaaggg tttaacccgc agctgtttac ctccggcacc
    12541 acctatgacg cctcttccgt accgctttat aaccgtctgg ttgaatttaa aatcggcacc
    12601 actgaagtca tcccgggcct cgctgaaaag tgggaagtca gtgaagacgg taaaacctat
    12661 accttccatc tgcgtaaagg tgtgaagtgg cacgacaata aagaattcaa accgacgcgc
    12721 gaactgaatg ccgatgatgt agtgttctcg ttcgatcgtc agaaaaacgc gcaaaacccg
    12781 taccataaag tctctggcgg cagctacgaa tacttcgaag gcatgggctt gccagagctg
    12841 atcagcgaag tgaaaaaggt ggacgataac accgttcagt ttgtgctgac tcgcccggaa
    12901 gcgccgttcc tcgctgacct ggcaatggac ttcgcctcta ttctgtcaaa agaatatgct
    12961 gacgcgatga tgaaagccgg tacaccggaa aaactggacc tcaacccaat cggtaccggc
    13021 ccgttccagt tacagcagta ccagaaagat tcccgtattc gctataaagc gtttgatggc
    13081 tactggggca ccaaaccgaa gatcgatacg ctggtcttct ctattactcc tgacgcttcc
    13141 gtgcgttacg cgaaattgca gaagaacgaa tgccaggtga tgccgtaccc gaacccggca
    13201 gatatcgccc gcatgaagca ggataaatcc atcaacctga tggaaatgcc ggggctgaac
    13261 gtcggttatc tctcgtataa cgtgcagaaa aaaccactgg atgacgtgaa agttcgccag
    13321 gctctgacct acgcggtgaa caaagacgcc atcatcaaag cggtttatca gggcgcgggc
    13381 gtatcagcga aaaacctgat cccgccaacc atgtggggct ataacgacga cgttcaggac
    13441 tacacttacg atcctgaaaa agcgaaagcc ttgctgaaag aagcgggtct ggaaaaaggt
    13501 ttctccatcg acctgtgggc aatgccggta caacgtccgt ataacccgaa tgctcgccgc
    13561 atggcggaga tgattcaggc agactgggcg aaagtgggcg tgcaggccaa aatcgtcacc
    13621 tacgaatggg gtgagtacct caagcgtgcg aaagatggcg agcatcagac ggtaatgatg
    13681 ggctggactg gcgataacgg ggatccggat aacttcttcg ccaccctgtt cagctgcgcc
    13741 gcctctgaac aaggctccaa ctactcaaaa tggtgctaca aaccgtttga agatctgatt
    13801 caaccggcgc gtgctaccga cgaccacaat aaacgtgttg aactgtacaa acaagcacag
    13861 gtcgtgatgc acgatcaggc tccggcactg atcatcgctc actccaccgt gtttgaaccg
    13921 gtacgcaaag aagttaaagg ctatgtggtt gatccattag gcaaacatca cttcgaaaat
    13981 gtctctatcg aataattaaa gccatacaag actgatggca aaggcaaaac tgcctgatgc
    14041 gctccgctta tcaggcctac gaaaactctg caatgtattg agtttgcacg attttgtagg
    14101 tcggataagg cgtttgcgcc gcatccggca taaacaaagc gctctttgtc aacaatctgc
    14161 atacccggtg gcgctgtgcc tctgcgtgca gcgccatccg gcagcaatac ttcattccct
    14221 gtcccacaag ggcgggaatt gatttgtgag caatacagac acgcagttcc aggctgcggg
    14281 tcactataga gaatccgggt tatgttgcag tttattctcc gacgtttggg actcgtcatc
    14341 cccacgttta tcggtattac ccttctcaca tttgcctttg tccacatgat cccgggcgat
    14401 ccggtgatga tcatggcggg cgaacgtggg atctccccag agcgtcacgc gcaattgctg
    14461 gctgaactcg gcttagataa accgatgtgg cagcagtatc tccattacat ttggggcgta
    14521 atgcacggtg atttaggcat ttccatgaaa agccgaattc cggtatggga agagttcgtg
    14581 ccgcgctttc aggccacgct ggaacttggc gtctgcgcga tgatttttgc gaccgcagtc
    14641 gggattccgg ttggtgtgct ggctgcggtt aaacgtggtt ccattttcga tcacacagcg
    14701 gttggcctgg cgctgaccgg ctactcgatg ccaattttct ggtggggcat gatgttgatc
    14761 atgctggttt cggtgcactg gaacctgacg cctgtctccg gtcgcgtgag cgacatggtg
    14821 ttcctcgatg attccaatcc gttaaccggt tttatgctga tcgacaccgc catctggggt
    14881 gaagacggta actttatcga tgccgtcgcc catatgatcc tgcccgccat tgtgctgggt
    14941 actattccgc tggcggtcat tgtgcgtatg acgcgctcct cgatgctgga agtgctgggc
    15001 gaagattata tccgcaccgc gcgtgccaaa ggtcttaccc ggatgcgggt gattatcgtc
    15061 catgcgctgc gtaacgcgat gctgccggtg gtgaccgtta tcggcctgca ggtgggaaca
    15121 ttgctggcgg gggcgattct gactgaaacc atcttctcgt ggccgggtct ggggcgctgg
    15181 ttgattgacg cactgcaacg ccgcgattat ccggtagtgc agggcggcgt attgctggtg
    15241 gcgacgatga ttatcctcgt caacctgctg gtcgatctgc tgtacggcgt ggtgaacccg
    15301 cgtattcgtc ataagaaata agggggcatc atgtcacagg ttactgaaaa taaagtgatt
    15361 agcgcaccgg tgccgatgac cccgttacag gagttctggc actattttaa acgcaacaaa
    15421 ggcgcggttg tcgggctggt ttacgtcgtc atcgtgctgt ttatcgcgat ctttgccaac
    15481 tggattgcac cctataaccc agcggaacag ttccgcgatg cactacttgc cccgcctgcc
    15541 tggcaggaag gcggcagcat ggcgcacttg ctgggcactg atgacgtggg gcgtgatgtg
    15601 ctgtcgcggc tgatgtacgg cgcgcgcttg tcgctgctgg ttggctgtct ggtagtggtg
    15661 ttatcgctga ttatgggcgt tattctcggc ctgatcgctg gttactttgg cggtctggtc
    15721 gataacatca tcatgcgcgt ggtcgatatc atgctggcgc tgccaagtct actgctggca
    15781 ctggtgctgg tggcgatttt cggcccgtcg attggtaacg ccgcgctggc gctaaccttc
    15841 gttgcgctgc cgcattatgt gcgcttaacc cgcgccgccg tgctggtgga agttaaccgc
    15901 gattacgtca ccgcgtctcg cgtggcgggt gccggggcaa tgcgccagat gtttattaac
    15961 atcttcccga actgccttgc gccgctgatt gttcaggcgt cgctcggttt ctctaacgcc
    16021 attctcgata tggctgctct tggtttcctc ggcatgggcg cgcagccgcc aacgccggag
    16081 tggggcacca tgctctctga cgtgttgcag ttcgcgcaaa gcgcctggtg ggtcgtgacc
    16141 ttcccgggtc tggcgatcct gctgacggtg ctggcattta acctgatggg tgacggtctg
    16201 cgtgacgcgc tcgatcccaa actgaagcag taagaggttc gagatggcgt tattaaatgt
    16261 agataaatta tcggtgcatt tcggcgacga aagcgcaccg ttccgcgccg tagaccgcat
    16321 cagctacagc gtaaaacagg gtgaagtggt cgggattgtg ggtgagtccg gctctggtaa
    16381 atcggtcagt tcgctggcga ttatggggct gattgattat ccgggccgcg taatggcgga
    16441 aaagctggag tttaacggtc aggatttgca gcgtatctca gaaaaagagc gccgcaacct
    16501 ggtgggtgcc gaagtggcaa tgatcttcca ggacccgatg accagcctta acccgtgcta
    16561 caccgtgggt ttccagatta tggaagcgat taaggtgcat caaggcggca acaaaagtac
    16621 ccgccgtcag cgagcgattg acctgctgaa tctggtcggt attcccgatc cggcatcgcg
    16681 tctggatgtt tacccgcatc agctttccgg cggcatgagc cagcgcgtga tgatcgccat
    16741 ggcgattgcc tgtcggccaa aactgctgat tgccgatgaa ccaaccactg cgctggacgt
    16801 gaccattcag gcgcaaatta tcgaactgct gctggagcta cagcagaaag agaacatggc
    16861 gctggtgtta attacccatg acctggcgct ggtggcggaa gcggcacata aaattatcgt
    16921 gatgtatgca ggccaggtag tagaaacggg cgatgcgcac gctatcttcc atgcgccgcg
    16981 tcacccgtat actcaggcat tgctgcgtgc gctgccggaa tttgctcagg acaaagaacg
    17041 tctggcgtcg ttgccaggtg tcgttcccgg caagtacgac cgcccgaacg gctgcctgct
    17101 taacccgcgc tgcccctacg ccactgacaa atgccgtgct gaagaaccgg cgctgaatat
    17161 gctcgctgac gggcgtcagt ccaaatgcca ttacccactt gatgatgccg ggaggccgac
    17221 actatgagta cgcatgaggc caccccgcaa caaccgctgt tgcaggctat cgacctgaaa
    17281 aaacattatc cggtgaagaa aggcatgttc gcgccggaac gtctggtgaa ggcgctggat
    17341 ggcgtttcgt ttaaccttga acgtggcaaa acgctggcag tagtaggtga atctggctgc
    17401 ggtaaatcga ccctaggtcg gttgctgacg atgattgaaa cgcccaccag cggcgaactg
    17461 tattaccagg ggcaggatct gctcaagcac gatccgcagg cgcagaagct acgtcggcag
    17521 aaaatccaga ttgtcttcca gaacccttac ggttcgctga atccgcgtaa aaaagttggg
    17581 cagattctcg aagagccgct gctgatcaac accagcttaa gcaaagtaca gcgtcgggaa
    17641 aaagctctgt cgatgatggc gaaagtcggc ctgaaaaccg agcattatga ccgctatccg
    17701 catatgttct ccggcggtca gcgtcagcgt atcgccattg cccgaggtct gatgctcgac
    17761 ccggatgtgg tgattgccga tgagccggtt tctgcgctgg acgtctcagt gcgtgcgcag
    17821 gtgctgaatc tgatgatgga tttgcagcag gaactggggc tatcttatgt ctttatctcg
    17881 cacgatctgt cggtggtgga gcacattgct gatgaagtga tggtgatgta cctgggccgc
    17941 tgcgtggaga aggggacgaa ggaccaaatc ttcaataacc cgcgccaccc gtacactcag
    18001 gcgctgctct ccgcgacacc acgcctgaac ccggacgatc gtcgcgaacg catcaaactt
    18061 accggtgaac tgccaagccc gctgaatcca ccgccgggct gcgccttcaa cgcccgctgt
    18121 cgtcgacgct tcggcccctg cacccagttg cagccgcagc taaaagacta cggcggtcaa
    18181 ctggtagctt gttttgccgt tgatcaggat gaaaatccgc agaagccagc tgtataactc
    18241 tcccgatgag atcagctttc atgctgatct catgtcaaat atgacctttt tatgctttag
    18301 ctatcagtta tatgtctttt ttgacctttt tctttgctaa tgggttatat atgagctaaa
    18361 ttataaccat taatgaaaaa tggttcattt gtgatactta acgaatttat ctgaccataa
    18421 atgatcttgt ttttgtcttt tatcaatcga aaggttcttt aatgatctgc tcaggaccac
    18481 aaaatctggc tcaagagttg aaagcgatcg gcgaccagct ccaggagtta cagaagaaac
    18541 ttattcaggg gccgaatatt agtcaaccac gccccctcct gaccattgag actccccgtc
    18601 atttggggga acaactgaac gcacgtcgca aagaattggg cattgattta tacacacttg
    18661 aactacaaac ggggatctca acgtccacgt taaagcgact ttttaaggat cctgaacagg
    18721 ttaaattcgg cagtgtgttc gcggtggcga atgttcttgg agtgaaacta tgcatcggcg
    18781 agtgaaagtg ttgctctatg gtcaggtagt gggtgaactc tctcagaacg acagtggttt
    18841 tttgttccag tatgccccgg attaccacgg accggcaatc tcaatcagcc tgcccgtcgc
    18901 acagcgtcag tttcccagtg agatgttgca tccttatttc gcctcactgg cgccagaagg
    18961 atggctgcgt caacgctaca gccagataca gcatcgtgac gaaaacgatc tgctgggaat
    19021 gcttattgat aacggcaaga atctgctggg ggccatccag attttacctt gggaggaata
    19081 atggcgaatt gtcgtattct gttaacgccg ttgaatgaac gtgacgaaca gcgcggatat
    19141 tcgacacagg ggttgaagcg actgagcggc actgcaaaat tgacccccag gctggggttt
    19201 acgcgaactc agtttgtgca ggaacttccg cgtcaacaaa aagggatgag tatttccggt
    19261 taccagccca aattgcaatt agtgctggat gaaggagagt tccgcgtcgt agatcatcag
    19321 ggaaatttta tccttaagcc ctctccagcc gactttcccg gtttggccga aaatgaacac
    19381 gcaacaatga cattgatgtc tcgtttggga tttgacgtac ctgtgcatgg cctgttgagt
    19441 tttgcaccac aatcagagga agagcttgaa tacgcatttg ttatccggcg ctatgaccgg
    19501 gataacaagg ggctgccagt gcatcaggaa cagcttgatg gcgcgatgca gattatggat
    19561 aaatatggca aaacggggaa tgataacgaa caatatgtca gctatgaaac gctggcccga
    19621 tttttagttg cccatgttaa cgacaacatc gcatttaaaa tcgatctctt tcgtcgcatt
    19681 gtttacgcct ggttgctggg taataacgac atgcatttac gaaactttgg cttagtgtac
    19741 tccgatggtt tgacgccagc gttagcgccg gtgtatgact ttgtttctgt cgctccctat
    19801 ccggaatatt ttcactcaaa ctatctggcg ctgccgttat tgacgcgaga agaaggcggt
    19861 cgggagctgg ctcctggatt tcacagtgat tatggcgagt atatcgggca ggactttttg
    19921 ctgttgggtg aatcaatggg gttagcgccc agattgctgg aaaaactctt tcaggatata
    19981 cgtaaagaaa atgccattgt tatggaaacg tatgagcagt cctttatgac tcaggatcat
    20041 atccaggctg tgctgcaatg ctatcgacat cgtctggggc tgctgtgagc agcccccgtt
    20101 acttttattc aatcagcttc aatagcggcg aaaagcacag caaaatgcca taaaacagga
    20161 taagccatgt ttttagcccg cgtagttttt tcagttgtga aactttagag atcaggaaaa
    20221 acggaatgag gcacgacaca atgccatata acgggcttcc caactgaaag aacaccagta
    20281 ccgatacacg aaacgaaacc caaatcgtca acgtcatgac gataaaagtg cagatcgcca
    20341 gagtcagcac gcgtgagtta attttcctgg tatcaataat tcggcttaac agattgagaa
    20401 taatgccttt aatggcctcg tggaaaccga gataaatgcc aaagaatgca gtcagtacgg
    20461 caaagatatt cagcaccgta gaggtgatat gaatgatatg cccagggatc acctgcgcgg
    20521 ccagtgccag tgccgagata ttttgttcaa aggcagaaac cgcctcttca tggctaattg
    20581 agaaggtaaa cgaaaaggca aaaaacagga tcaccgcgat gagcgtgaca taactaattc
    20641 ggtgggtacg cagcgcgagc cgggttgcca gtactttatc cgcttcccgt ttacgatagg
    20701 caatattcat tgggttaagt acctgaataa ataccgcgga aaagaagcaa aatggaatag
    20761 taagcaggac atcgcggaaa aagtcggagg cttgcgggaa ggcggttata ttggcgaaat
    20821 tccagtgcgg gatcatcgca aaaccgaaca cgacaataat ccctactttg accaccacca
    20881 ttggtccgga aatcttaaac agtaatcgtt caccaccaga cgcaatcgcc accagtacgg
    20941 cgaaaatggc gactttataa aatagagatt gagaaagatc ggcatcggtt aaaccgaagg
    21001 ttttcaggta cgaggcgctg tcgaaaacca cggagagaga gtagataaaa atcccgtgga
    21061 taatcatcaa aaagtagata accccgagga aaattcccca gttcttcccc aggtaatgac
    21121 tgataatatc ggtgtagtca ttacaggaat cgctttcaga aagggttttt aaataaatgt
    21181 cctgtactac ccaggtggca ggataagcaa tgatcgctgc ggtaataaat acccaaattc
    21241 ccttcaagcc aatttgtact ggcatcagca cggttccggc accaatcgcc atgcctatgc
    21301 ataataaaac ccagccgaag tcgtagcgtg taaacggcaa tttctgatcg tgtttcggta
    21361 gtgtgttgtg ctgcataaag tcctgtccag aaagaagtcg gcctcgcaaa aatgaataaa
    21421 cccagtttct cttgcgcaac aggtggagaa gtcgaatacg cagtatgtaa aaaatgaggc
    21481 aacagattca ctggggaggt cgcgaggagg cgcaattcta caaaattgcg catttcacta
    21541 ctagtctgga gtcagcccgg atatgagctg caactattgc aaataaagaa attaagctgg
    21601 aaggcatcac gcaatgagga agattgccgg gtgctgacgg caaaaagcaa tcgtaaaatc
    21661 agcggcaagc cggaagggtt ccggtgaggc gcaatgttgc gggggcttta tccctggtgg
    21721 cattggttgc tggagagaga aaacccccgc acgttgcagg tatgcacctg acaacatcac
    21781 gggggccatt cttgcatcta gaccattcaa gaatagccgc gaaacgttgt cattacaaca
    21841 caggcggcta tatgacgctc gcagagctgg gcatggcctt ctggcatgat ttagcggctc
    21901 cggtcattgc tggcattctt gccagtatga tcgtgaactg gctgaacaag cggaagtaac
    21961 gtgtcatgcg ggcgtcaggc tgccgtaatg gcaatttgcg cccggaccag gccgcagagg
    22021 tgaaactctg cggccttttt cgtaagaaaa atgcaacggt tgcaagcgga taatattctg
    22081 cggaatgcgc agcacagagt gaagaagtga cggattccgg aacagaaaaa cagtcgtaga
    22141 atcagcgtca agccggaagg gttccggtga ggcgcaatgt tgcgggggct taatccctgg
    22201 tggcattggt tgctggagag agaaaacccc cgcacgttgc aggtatgcac ctgacaacat
    22261 cacgggggct aatcttgaac ctgaacaact caagaatagc cgcgaaacgt tgtcattaca
    22321 acacaggcgg ctatatgacg ctcgcagagc tgggcatggc cttctggcat gatttagcgg
    22381 ttccggtcat tactggcatt cttgccagta tgatcgtga
//
LOCUS       DAHHTG010000074        21637 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.074, whole genome shotgun sequence.
ACCESSION   DAHHTG010000074 DAHHTG010000000
VERSION     DAHHTG010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21637)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21637)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21637
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.074"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            complement(240..1133)
                     /locus_tag="NBI74_002814"
     CDS             complement(240..1133)
                     /locus_tag="NBI74_002814"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HCD1933114.1"
                     /translation="MKTLVVALGGNALLQRGEALTAKNQYRNIASAVPALARLARSYR
                     LAIVHGNGPQVGLLALQNLAWKEVEPYPLDVLVAESQGMIGYMLAQGLSAQPQMPPVT
                     TVLTRIEVSPDDPAFLQPEKFIGPVYQPEEQEALEAAYGWQMKRDGKYLRRVVASPQP
                     RKILDSEAIELLLKEGHVVICSGGGGVPVTEDGAGSEAVIDKDLAAALLAEQINADGL
                     VILTDADAVYENWGTPQQRAIRHATPDELAPFAKADGSMGPKVTAVSGYVRSRGKPAW
                     IGALSRIEETLAGEAGTCISL"
     gene            complement(1130..1945)
                     /locus_tag="NBI74_002815"
     CDS             complement(1130..1945)
                     /locus_tag="NBI74_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
                     /protein_id="HCD1933115.1"
                     /translation="MTIIHPLLASRSAPNYRQSWRLAGVWRRAINLMTESGELLTLHR
                     QGSGFGPGGWVLRRAQFDALCGGLCGNERPQVVAQGIRLGRFTVKQPQRYCLLRITPP
                     AHPQPLAVAWMQRAEETGLFGPLALAASDPLPAELRQFRHCFQAALNGVKTDWRHWLG
                     KGPGLTPSHDDTLSGMLLAAWYYGALDARSGRPFFAFSDNLQLVTTAVSVSYLRYAAQ
                     GYFASPLLHFVHALSCPKRTAAAIDSLLALGHTSGADTLLGFWLGQQLLQGKP"
     gene            complement(1956..3215)
                     /locus_tag="NBI74_002816"
     CDS             complement(1956..3215)
                     /locus_tag="NBI74_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009029994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HCD1933116.1"
                     /translation="MFTSVAQANAAVIEQIRRARPHWLDVQPASSLISELNKGKTLLH
                     AGPPMRWQEMTGPMKGACVGACLFEGWAKDEAQALAILEQGEVNFIPCHHVNAVGPMG
                     GITSASMPMLVVENVTDGNRAYCNLNEGIGKVMRFGAYGEDVLTRHRWMRDVLMPVLS
                     AALGRMERGIDLTAMMAQGITMGDEFHQRNIASSALLMRTLAPQIARLDHDKQHIAEV
                     MDFLSVTDQFFLNLAMAYCKAAMDAGAMIRAGSIVTAMTRNGNMFGIRVSGLGERWFT
                     APVNTPQGLFFTGFSQDQANPDMGDSAITETFGIGGAAMIAAPGVTRFVGAGGMEAAR
                     AVSEEMAEIYLERNMQLQIPGWDFQGACLGLDIRRVVETGITPLINTGIAHKEAGIGQ
                     IGAGTVRAPLACFEQALEALAESMGIG"
     gene            complement(3225..4892)
                     /locus_tag="NBI74_002817"
     CDS             complement(3225..4892)
                     /locus_tag="NBI74_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase FdrA"
                     /protein_id="HCD1933117.1"
                     /translation="MIHAFIKKGCFQDSVSLMIISRKLSESENVDDVSVMMGTPANKA
                     LLDTTGFWHDDFNNATPNDICVAIRSEAADAGIAQAVMQQLEEALKQLAQGSGSSQAL
                     TQVRRWDSASQKLPDANLALISVAGEYAAELANQALDRNLNVMMFSDNVTLEDEIQLK
                     TRAREKGLLVMGPDCGTSMIAATPLAFANVMPEGNIGVIGASGTGIQELCSQIALAGE
                     GITHAIGLGGRDLSREVGGISALTALEMLSVDEKSEVLAFVSKPPAEAVRLKIVNAMK
                     ATGKPTVALFLGYTPAVARDENVWFASSLDEAARLACLLSRVTARRNAIAPVSSGFIC
                     GLYTGGTLAAEAAGLLAGHLGVEADDTHQHGMMLDADGHQILDLGDDFYTVGRPHPMI
                     DPTLRNLLIADLGAKPQVRVLLLDVVIGFGATADPAASLVSSWQKACAARSDNQPLYA
                     IATVTGTERDPQCRSQQIATLEDAGIAVVSSLPEATLLAAALIHPLSPATQQHTPSLL
                     ENVAVINIGLRSFALELQSASKPVVHYQWSPVAGGNKKLARLLERLQ"
     gene            5209..6258
                     /gene="allD"
                     /locus_tag="NBI74_002818"
     CDS             5209..6258
                     /gene="allD"
                     /locus_tag="NBI74_002818"
                     /EC_number="1.1.1.154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ureidoglycolate dehydrogenase"
                     /protein_id="HCD1933118.1"
                     /translation="MKISRETLHQLIENKLCQAGLKREHAATVAEVLVYADARGIHSH
                     GAVRVEYYAERISKGGTNREPEFRLEETGPCSAILHADNAAGQVAAKMGMEHAIKTAQ
                     QKGVAVVGISRMGHSGAISYFVQQAARAGLIGISLCQSDPMVVPFGGAEIYYGTNPLA
                     FAAPGEGDEILTFDMATTVQAWGKVLDARSRNMSIPDTWAVDKNGAPTTDPFAVHALL
                     PAAGPKGYGLMMMIDVLSGVLLGLPFGRQVSSMYDDLHAGRNLGQLHIVINPNFFSSS
                     ELFRQHLSQTMRELNTITPAPGFNQVYYPGQDQDIKQRKAAVEGIEIVDDIYQYLISD
                     ALYNTSYETKNPFAQ"
     gene            6280..7515
                     /gene="allC"
                     /locus_tag="NBI74_002819"
     CDS             6280..7515
                     /gene="allC"
                     /locus_tag="NBI74_002819"
                     /EC_number="3.5.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="allantoate deiminase"
                     /protein_id="HCD1933119.1"
                     /translation="MITHFRQAIEETLPWLSSFGADPAGGMTRLLYSPEWLETQQQFK
                     KRMAASGLETRFDEVGNLYGRLNGTEYPQEVVLSGSHIDTVVNGGNLDGQFGALAAWL
                     AIDWLKTQYGAPLRTVEVVAMAEEEGSRFPYVFWGSKNIFGLANPDDVRNICDAKGNS
                     FVDAMKACGFTLPNAPLTPRQDIKAFVELHIEQGCVLESNGQSIGVVNAIVGQRRYTV
                     TLNGESNHAGTTPMGYRRDTVYAFSRICHQSVEKAKKMGDPLVLTFGKVEPRPNTVNV
                     VPGKTTFTIDCRHTDAAVLRDFTQQLENDMRAICDEMDIGIDIDLWMDEEPVPMNKEL
                     VATLTELCESEKLNYRVMHSGAGHDAQIFAPRVPTCMIFIPSINGISHNPAERTNITD
                     LAEGVKTLALMLYQLAWQK"
     gene            7526..8311
                     /gene="allE"
                     /locus_tag="NBI74_002820"
     CDS             7526..8311
                     /gene="allE"
                     /locus_tag="NBI74_002820"
                     /EC_number="3.5.3.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(S)-ureidoglycine aminohydrolase"
                     /protein_id="HCD1933120.1"
                     /translation="MGYLNNVTGYRDDLLANRAIVKHGNFALLTPDGLVKNIIPGFVN
                     CDATILSTPKLGASFVDYLVTLHQNGGNQQGFGGEGIETFLYVISGNITAKAEGKTFA
                     LSEGGYLYCPPGSLMTFVNAQAEDSQIFLYKRRYIPVEGHAPWLVSGNASELERIHYE
                     GMDDVILLDFLPKELGFDMNMHILSFAPGASHGYIETHVQEHGAYILSGQGVYNLDNN
                     WIPVKKGDYIFMGAYSLQAGYGVGRGEAFSYIYSKDCNRDVEI"
     gene            complement(8439..9584)
                     /gene="glxK"
                     /locus_tag="NBI74_002821"
     CDS             complement(8439..9584)
                     /gene="glxK"
                     /locus_tag="NBI74_002821"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate 3-kinase"
                     /protein_id="HCD1933121.1"
                     /translation="MKIVIAPDSFKESLSAEKCCQAIKAGFSTIFPDAHYICLPIADG
                     GEGTVEAMVAATDGNIVKLEVCGPMGEKVNAFYGITGDGKTAVIEMAAASGLMLVVPE
                     KRNPLLASSFGTGELILHALDNGIRHIILGIGGSATVDGGMGMAQALGVRFLDADGQP
                     LAANGGNLARVASIEMNECDPRLANCHIEVACDVDNPLVGARGAAAVFGPQKGATPEM
                     VEELEQGLQNYARVLQQLTEINVCQMAGGGAAGGMGIAAAVFLNADIKPGIEIVLNAV
                     NLAQAVQGAALVITGEGRIDSQTAGGKAPLGVASVAKQFNVPVIGIAGVLGDGVEVVH
                     QYGIDAVFSILPRLAPLAEVLASGETNLFNSARNIACAIKIGQGIKN"
     gene            complement(9606..10907)
                     /locus_tag="NBI74_002822"
     CDS             complement(9606..10907)
                     /locus_tag="NBI74_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil/xanthine transporter"
                     /protein_id="HCD1933122.1"
                     /translation="MFNFAVGRESLLSGFQWFFFIFCNTVVVPPTLLSAFQLPQSSLL
                     TLTQYAFLATALACFAQAFCGHRRAIMEGPGGLWWGTILTITLGEASRGTPINDIATS
                     LAVGIALSGVLTMLIGFSGLGHRLARLFTPSVMVLFMLMLGAQLTTIFFKGMLGLPFG
                     IAAPNFKIQLPPFALSVAVMCLVLAMIIFLPQRFARYGLLVGTITGWLLWYFCFPSSH
                     SLSGELHWQWFPLGSGGALSPGIILTAVITGLVNISNTYGAIRGTDVFYPQQGAGNTR
                     YRRSFVATGFMTLITVPLAVIPFSPFVSSIGLLTQTGDYSRRSFIYGSVICLLVALVP
                     ALTRLFCSIPLPVSSAVMLVSYLPLLFSALVFSQQITFTARNIYRLALPLFVGIFLMA
                     LPPVYLQDLPLTLRPLLSNGLLVGILLAVLMDNLIPWERIE"
     gene            complement(10963..12324)
                     /gene="allB"
                     /locus_tag="NBI74_002823"
     CDS             complement(10963..12324)
                     /gene="allB"
                     /locus_tag="NBI74_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="allantoinase AllB"
                     /protein_id="HCD1933123.1"
                     /translation="MSFDLIIKNGTVILENEARVVDIAVKDGKIAAIGQDLGDAKDVM
                     DASGLVVSPGMVDAHTHISEPGRSHWEGYETGTRAAAKGGITTMIEMPLNQLPATVDR
                     ASIELKFDAAKGKLTIDAAQLGGLVSYNIDRLHELDEVGVVGFKCFVATCGDRGIDND
                     FRDVNDWQFFKGAQKLGELGQPVLVHCENALICDALGEEAKREGRVTAHDYVASRPVF
                     TEVEAIRRVLYLAKVAGCRLHVCHVSSPEGVEEVTRARQEGQDVTCESCPHYFVLDTD
                     QFEEIGTLAKCSPPIRDLENQKGMWEKLFNGEIDCLVSDHSPCPPEMKAGNIMKAWGG
                     IAGLQSCMDVMFDEAVQKRGMSLPMFGKLMATNAADIFGLQQKGRIAPGKDADFVFIQ
                     PNSSYVLTNDDLEYRHKVSPYVGRTIGARITKTILRGDVIYDIEQGFPVAPKGQFILK
                     HQQ"
     gene            complement(12384..13838)
                     /locus_tag="NBI74_002824"
     CDS             complement(12384..13838)
                     /locus_tag="NBI74_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative allantoin permease"
                     /protein_id="HCD1933124.1"
                     /translation="MEHQRKLFQQRGYSEDLLPKTQSQRTWKTFNYFTLWMGSVHNVP
                     NYVMVGGFFILGLSTFSIMLAIILSAFFIAAVMVLNGAAGSKYGVPFAMILRASYGVR
                     GALFPGLLRGGIAAIMWFGLQCYAGSLACLILIGKIWPGFLTLGGDFTLLGLSLPGLI
                     TFLLFWLVNVGIGFGGGKVLNKFTAILNPCIYIVFGGMAIWAISLVGIGPIFDYIPGG
                     IQKAENSGFLFLVVINAVVAVWAAPAVSASDFTQNAHSFREQALGQTLGLVVAYILFA
                     VAGVCIIAGASIHYGADTWNVLDIVQRWDSLFASFFAVLVILMTTISTNATGNIIPAG
                     YQIAAIAPTKLTYKNGVLIASIISLLICPWKLMENQDSIYLFLDIIGGMLGPVIGVMM
                     AHYFVVMRGQINLDELYTAPGDYKYYDNGFNLTAFSVTLVAVILSLGGKFIPFMEPLS
                     RVSWFVGVIVAFAAYALLKKRTTAEKTGEQKTIG"
     gene            complement(14008..14886)
                     /gene="glxR"
                     /locus_tag="NBI74_002825"
                     /pseudo
     CDS             complement(14008..14886)
                     /gene="glxR"
                     /locus_tag="NBI74_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415042.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
     gene            complement(14986..15762)
                     /gene="hyi"
                     /locus_tag="NBI74_002826"
     CDS             complement(14986..15762)
                     /gene="hyi"
                     /locus_tag="NBI74_002826"
                     /EC_number="5.3.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxypyruvate isomerase"
                     /protein_id="HCD1933125.1"
                     /translation="MLRFSANLSMLFGEYDFLARFEKAAQCGFRGVEFMFPYDYDIEE
                     LKQVLASNKLEHTLHNLPAGDWAAGERGIACIPGREEEFRDGVAAAIRYARALGNKKI
                     NCLVGKTPTGFSSEQIHTTLVENLRYAANMLMKEDILLLIEPINHFDIPGFHLTGTRQ
                     ALKLIDDVGCCNLKIQYDIYHMQRMEGELTNTMTQWADKIGHLQIADNPHRGEPGTGE
                     INYEYLFTVIENSDYNGWVGCEYKPQTTTEAGLRWMDPYR"
     gene            complement(15775..17556)
                     /gene="gcl"
                     /locus_tag="NBI74_002827"
     CDS             complement(15775..17556)
                     /gene="gcl"
                     /locus_tag="NBI74_002827"
                     /EC_number="4.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate carboligase"
                     /protein_id="HCD1933126.1"
                     /translation="MAKMRAVDAAMYVLEKEGITTAFGVPGAAINPFYSAMRKHGGIR
                     HILARHVEGASHMAEGYTRATAGNIGVCLGTSGPAGTDMITALYSASADSIPILCITG
                     QAPRARLHKEDFQAVDIEAIAKPVSKMAVTVREAALVPRVLQQAFHLMRSGRPGPVLV
                     DLPFDVQVAEIEFDPDMYEPLPVYKPAASRMQIEKAVEMLIQAERPVIVAGGGVINAD
                     AAVLLQQFAELTSIPVIPTLMGWGCIPDDHELMAGMVGLQTAHRYGNATLLASDMVFG
                     IGNRFANRHTGSVEKYTEGRKIVHIDIEPTQIGRVLCPDLGIVSDAKAALTLLVEVAQ
                     EMQKAGRLPCRKEWVADCQQRKRTLLRKTHFDNVPVKPQRVYEEMNKAFGRDVCYVTT
                     IGLSQIAAAQMLHVFKDRHWINCGQAGPLGWTIPAALGVCAADPERNVVAISGDFDFQ
                     FLIEELAVGAQFNIPYIHVLVNNAYLGLIRQSQRAFDMDYCVQLAFENINSSEVNGYG
                     VDHVKVAEGLGCKAIRVFKPEDIAPAFEQAKILMAQYRVPVVVEVILERVTNISMGSE
                     LDNVMEFEDIADNAADAPTETCFMHYE"
     gene            complement(17646..18461)
                     /gene="allR"
                     /locus_tag="NBI74_002828"
     CDS             complement(17646..18461)
                     /gene="allR"
                     /locus_tag="NBI74_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000141283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor AllR"
                     /protein_id="HCD1933127.1"
                     /translation="MTEVRRRGRPGQAEPVAQKGAQALERGIAILQYLEKSGGSSSVS
                     DISLNLDLPLSTTFRLLKVLQAADFVYQDNQLGWWHIGLGVFNVGAAYIHNRDVLSVA
                     GPFMRRLMLLSGETVNVAIRNGNEAVLIGQLECKSMVRMCAPLGSRLPLHASGAGKAL
                     LYPLAEEELMSIILQTGLQQFTPTTLVDMPTLLKDLEQARELGYTVDKEEHVVGLNCI
                     ASAIYDDVGSVVAAISISGPSSRLTEDRFVSQGELVRDTARDISTALGLKAHL"
     gene            complement(18539..19021)
                     /gene="allA"
                     /locus_tag="NBI74_002829"
     CDS             complement(18539..19021)
                     /gene="allA"
                     /locus_tag="NBI74_002829"
                     /EC_number="4.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ureidoglycolate lyase"
                     /protein_id="HCD1933128.1"
                     /translation="MKLQVLPLSQEAFSAYGDVIETQKRDFFHINNGLVERYHDLALV
                     EILEQDRTLISINRAQPANLPLTIHELERHPLGTQAFIPMKGEVFVVVVALGDDKPDL
                     STLRAFITNGEQGVNYHRNVWHHPLFAWQRVTDFLTIDRGGSDNCDVESIPEQELCFA
                     "
     gene            19251..20177
                     /gene="allS"
                     /locus_tag="NBI74_002830"
     CDS             19251..20177
                     /gene="allS"
                     /locus_tag="NBI74_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AllS"
                     /protein_id="HCD1933129.1"
                     /translation="MFDPETLRTFIAVAETGSFSKAAERLCKTTATISYRIKLLEENT
                     GVALFFRTTRSVTLTAAGEHLLCQARDWLSWLESMPSELQQVNDGVERQVNIVINNLL
                     YNPQAVARLLAWLNERYPFTQFHISRQIYMGVWDSLLYEGFSLAIGVTGTEALANTFS
                     LDPLGSVQWRFVMAADHPLANVEEPLTEAQLRRFPAVNIEDSARTLTKRVAWRLPGQK
                     EIIVPDMETKIAAHLAGVGIGFLPKSLCQSMLDNQQLVSRVIPTMRPPSPLSLAWRKF
                     GSGKAVEDIVTLFTQRRPEISGFLEIFGNPRS"
     gene            20246..21352
                     /gene="mnmH"
                     /locus_tag="NBI74_002831"
     CDS             20246..21352
                     /gene="mnmH"
                     /locus_tag="NBI74_002831"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001157950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HCD1933130.1"
                     /translation="MQERHTEQDYRALLIADTPIIDVRAPIEFEQGAMPAAINLPLMN
                     NDERAAVGICYKQQGSDAALALGHKLVAGEIRQQRMDAWRAACLQNPHGILCCARGGQ
                     RSHIVQSWLHDAGIDYPLVEGGYKALRQTAIQATIELAQKPIVLIGGCTGSGKTLLVQ
                     QQPNGVDLEGLARHRGSAFGRTLQPQLSQASFENMLAAEMLKTDAHQDLRLWVLEDES
                     RMIGSNHLPECLRERMTQATIAVVEDPFEIRLERLNEEYFLRMHHDFTHAYGDEQGWQ
                     EYCEYLHHGLSAIKRRLGLQRYNELAARLDAALTTQLTTGSTDGHLAWLVPLLEEYYD
                     PMYRYQLEKKAEKVVFRGEWAEVAEWVKTYACGY"
     gene            complement(21404..>21637)
                     /locus_tag="NBI74_002832"
     CDS             complement(21404..>21637)
                     /locus_tag="NBI74_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001606949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2931 family protein"
                     /protein_id="HCD1933131.1"
                     /translation="YLRYLLIGLAPEGKIGVWLEKPDKPNIRLTDKQILIETVSGEKM
                     EMCNGRSAYKHGYSYPESTKNFIKDKKYPYGNW"
BASE COUNT         5465 a         5909 c         5238 g         5025 t
ORIGIN      
        1 cgcatcaggc atggtgtgcc gattgccgga tgcggcgtga acgccttatc cggcctaccg
       61 ttccgacacc gactgtaggc ctgataagac gcgccagcat cgcatcaggc atggtgtgcc
      121 gattgccgga tgcggcgtga acgccttatc cggcctaccg ttccgacacc gcctgtagac
      181 ctgataagac gcgccagcat cgcatcaggc tagatgtgcc aattgcctgg tgcccatcac
      241 tacagcgaaa tacaagtccc cgcttcgccc gccagcgtct cttcaatccg cgataacgcc
      301 ccaatccacg cgggtttacc acggcttctg acatagccac tcaccgccgt taccttcggc
      361 cccatcgaac catcggcttt ggcaaatggc gctaactcat ccggtgtggc atggcgaatg
      421 gcacgttgct gcggcgttcc ccagttttca tataccgcat cagcatcggt gaggatcacc
      481 agtccatcgg cattaatctg ctcggcgagc aacgcagcgg cgagatcttt atcaatcacc
      541 gcttcactcc ctgccccgtc ttccgtcaca ggcacaccgc cgccgccact gcaaatcacc
      601 acatgcccct ctttgagcaa caactcgatg gcttcgctgt cgagaatttt acgcggttgc
      661 ggagacgcca ccacccggcg taaatattta ccatcacgtt tcatctgcca gccgtaagcc
      721 gcttccagtg cctcttgttc ttctggctga taaaccggac caataaattt ctctggctgt
      781 aaaaacgccg gatcatcagg cgaaacctca atgcgcgtca gcaccgtcgt gacaggcggc
      841 atctgcggct gtgcgctcaa gccctgcgcc agcatatagc caatcatccc ctggctttcc
      901 gccaccagta catccagcgg atacggttct acctctttcc acgccagatt ctgtaacgcc
      961 agcagcccca cctgcggccc gttgccgtga acaatcgcca gccgataaga acgggccagg
     1021 cgcgccagcg cgggtacagc actggcaata ttgcgatatt gattttttgc cgtcagcgcc
     1081 tcaccgcgct ggagtaaggc gttgccccca agagccacaa ccagtgtttt catggtttcc
     1141 cttgtaataa ttgttggcca agccagaacc ccagcagcgt atcggcccct gacgtatgcc
     1201 ccagcgccag cagcgaatcg atcgccgctg cggtacgttt cgggcaactc agagcatgaa
     1261 caaagtgcag gagtggcgag gcgaaatatc cttgcgcggc ataacgtaaa taactgacgc
     1321 tcaccgcagt ggtaacgagt tgaagattgt cggaaaaggc aaaaaacggg cgaccggagc
     1381 gcgcatctaa agccccataa taccaggccg ccagcagcat tccgctcagc gtgtcatcat
     1441 gacttggcgt taatccgggg cctttaccca gccagtgccg ccagtcggtc ttaacgccat
     1501 tgagcgcggc ctgaaaacag tgacgaaact ggcgtaattc agcaggcagc ggatcgcttg
     1561 ccgccaatgc cagtggcccg aaaagcccgg tttcctccgc gcgttgcatc catgcaacgg
     1621 caagcggttg aggatgcgca ggcggcgtaa tacgtagcaa acaataacgc tgtggctgtt
     1681 taaccgtgaa acgcccgagg cgaatccctt gtgccacaac ctgtggtcgt tcattgccgc
     1741 ataatccacc gcataacgca tcgaattgcg cgcggcgaag cacccatccg ccggggccga
     1801 aaccactccc ctgacgatgc agcgttaaca gttcgccgct ttccgtcatc aggttaatcg
     1861 cccgccgcca cactcccgct aaccgccagg actggcgata attcggtgcg ctacggctgg
     1921 caagtagagg atggatgatc gtcattgtgc gttcctcaac caatccccat gctttcagcc
     1981 agtgcttcca gcgcctgttc aaagcacgcc agcggtgccc gcacggtgcc tgcgccaatc
     2041 tgcccgatcc ccgcctcttt atgggcgata ccggtattga tgagtggcgt aatgccggtt
     2101 tctaccacgc gacgaatgtc cagccccagg cacgccccct ggaaatccca gccggggatc
     2161 tgcaattgca tattgcgttc aaggtaaatt tccgccatct cttcagatac cgctctggcc
     2221 gcttccatgc cacccgcacc gacaaagcgc gttacgccag gcgctgcgat cattgccgct
     2281 ccgccaatac caaaggtttc ggtaatcgcg ctgtcgccca tatccgggtt cgcctgatcc
     2341 tgcgagaagc cggtgaaaaa cagaccttgg ggagtgttta caggcgcagt aaaccagcgt
     2401 tcacccagcc cgcttacccg aatcccgaac atattgccgt tgcgggtcat agccgtaacg
     2461 atgctgcctg cgcggatcat cgcgcctgca tccatcgccg ccttgcagta ggccatcgcg
     2521 agattgagga agaactgatc ggtcacgctg aggaaatcca tcacttccgc aatgtgctgt
     2581 ttatcatgat cgaggcgagc aatttgtggg gccagcgtac gcattaacag tgctgaggaa
     2641 gcaatattgc gttgatggaa ctcatcgccc atggtaatgc cctgcgccat cattgccgtg
     2701 agatcgatac cgcgctccat gcgccccagc gccgcgctta atactggcat taacacatcg
     2761 cgcatccagc gatggcgagt caggacatct tcgccgtaag cgccaaaacg catcactttg
     2821 ccgatacctt cgttgaggtt gcagtacgcc cggttgccgt cggtcacgtt ctcgaccacc
     2881 agcatcggca tactggcaga agtaatgcca cccattggcc cgacggcatt cacatggtga
     2941 caaggaatga agttcacttc cccctgctcc agtattgcca gcgcctgcgc ttcatctttc
     3001 gcccagcctt cgaacagaca tgcgcccacg cacgcccctt tcatgggtcc ggtcatctcc
     3061 tgccagcgca ttggcggccc ggcgtgaagc agtgttttgc ccttgtttag ttcgctgata
     3121 agtgaagaag ccggttgcac atccagccag tgtggacgag cgcgacgaat ttgttcaata
     3181 accgcagcat tggcttgcgc cactgatgta aacatgggaa cctcttattg caaacgttct
     3241 aataaacgag ccagtttttt attgccgccg gcgactggcg accattggta atgcacaacc
     3301 ggtttgctgg cgctttgtag ctccagcgca aagctgcgta atccgatgtt aatcacggcg
     3361 acgttttcca gtaatgacgg tgtgtgttgc tgtgtggcag gcgagagcgg atgaattaac
     3421 gccgccgcca gcaaggtggc ttccggtagc gaactcacta ccgcgatccc cgcatcttcc
     3481 agcgtggcaa tttgctgcga gcgacattgc ggatcacgtt cagttcctgt caccgtggca
     3541 atggcataca gtggttgatt gtctgaacgc gcggcacagg ctttttgcca tgagctaacc
     3601 agcgaggcgg caggatcggc ggtcgcaccg aagccaatca cgacatcaag cagcaacacg
     3661 cgcacttgcg gtttagcgcc gagatcggca attaacaggt tgcgtaaggt cgggtcgatc
     3721 atcggatggg gacgcccgac ggtgtaaaaa tcatcgccca ggtcgaggat ctgatgtccg
     3781 tcggcgtcca gcatcatgcc atgttgatgg gtatcgtcgg cttccacgcc aaggtgtccg
     3841 gcaagtaatc ccgccgcttc agcagccagc gtaccgccgg tatacaaacc gcaaataaat
     3901 ccgctgctga caggcgctat cgcgttacgt cgcgcagtga cacgtgaaag cagacaagcc
     3961 agacgagcgg cctcatccag cgaggaggca aaccagacat tctcgtcgcg ggccaccgcc
     4021 ggggtataac ctaaaaacag cgccaccgtc ggtttgccag ttgctttcat ggcattaaca
     4081 attttcagac gcacagcttc ggcaggtggt tttgaaacaa atgccagcac ttcgcttttc
     4141 tcgtctacac tgagcatttc cagcgccgtt agcgcactga tgccgcccac ttcacggctg
     4201 agatcgcgcc cgccaagacc aatcgcgtga gtaattccct cccctgccag cgcaatctgt
     4261 gagcacagct cctgaatccc ggtaccggaa gcgcctatga cgccaatgtt gccttccggc
     4321 atcacattag caaaagccag cggcgtggcg gcaatcatcg acgtaccgca gtctggcccc
     4381 atcaccagca agcctttttc ccgcgcgcgg gttttaagtt ggatttcatc ttccagcgtg
     4441 acgttatcgg agaacatcat cacgttgagg ttgcgatcca gcgcctggtt cgccagctcc
     4501 gccgcatact caccagccac tgaaatcagc gccagattgg catcgggtaa tttctgactg
     4561 gcactgtccc agcgacgcac ctgcgtcaac gcctggctgc tgcctgaacc ctgcgccagt
     4621 tgttttagcg cctcttcaag ctgctgcata accgcctgcg cgatccccgc atccgccgct
     4681 tcgctacgaa ttgccacgca aatatcgttc ggcgtggcgt tattaaaatc gtcatgccag
     4741 aaacctgtag tatctaataa cgctttattg gcgggcgtac ccatcattac ggaaacatca
     4801 tcaacatttt ctgattcgct gagttttcgt gaaataatca ttaaactgac cgaatcctga
     4861 aaacaccctt ttttaataaa ggcgtggatc ataacaactc cttaaaaatt gcacccatgg
     4921 caattgcacg agcagtgtta atgtggcgat tacggtatcg atgacaacga gtaaataagg
     4981 tgatgaatat cacacgggga ttattaacaa aagaattcaa ataaccgcca gactattacg
     5041 cgttattttt taataagcat ggttgtttta aaaaccaggt taaaagcacg aagattatca
     5101 aataaattct aactatattt ttttgcctgt ctggatcaca taatccagat attttccctg
     5161 tatgttaatt gcagtcatgc ttcacaccgt cgttaaaaag gaagacagat gaaaatcagt
     5221 cgggaaacac tccaccagct aatcgagaat aaactctgcc aggctgggtt aaaacgtgag
     5281 cacgctgcaa ccgtggctga agtattggtt tacgccgatg ccagagggat ccactctcat
     5341 ggcgcggtgc gcgttgaata ctacgcggaa cgcatttcaa aaggcggcac caaccgcgaa
     5401 ccggagtttc gtcttgagga aaccggaccg tgctcggcaa ttttacatgc cgacaatgcc
     5461 gccggacagg tcgcggcgaa aatgggtatg gaacatgcca tcaaaaccgc ccagcaaaaa
     5521 ggcgttgcgg tggtcggtat cagccggatg ggtcacagcg gcgcaatctc ttattttgtg
     5581 cagcaggctg cccgcgccgg attgattggc atttcgctgt gccagtccga tccaatggtg
     5641 gtgccgtttg gcggcgcgga aatttactac ggcactaacc cactggcctt tgccgcgccg
     5701 ggagaaggcg atgagatcct taccttcgat atggcgacta ccgtacaggc atggggaaaa
     5761 gtactcgacg cccgctcgcg caatatgtct atcccggata cctgggcagt cgataaaaac
     5821 ggtgcaccaa caaccgatcc gttcgcggta catgctctgc tccccgccgc tgggccaaaa
     5881 gggtatggcc tgatgatgat gattgacgtc ctctcaggcg tcttactcgg cttaccgttc
     5941 gggcgacagg ttagttcgat gtatgacgat ttacacgccg ggcgtaattt ggggcaatta
     6001 catatagtta ttaacccgaa ctttttctcc tccagcgaat tattccgtca acatcttagc
     6061 cagaccatgc gcgaattaaa taccattacc cccgcgcccg gttttaatca ggtttattat
     6121 cccggacagg atcaggatat taaacaacgc aaagccgccg tcgaaggcat cgaaattgtt
     6181 gatgatattt accagtattt aatttccgac gcgctttata acacgtcata cgaaacgaaa
     6241 aatccctttg cgcaataatt attgagacag gactttctta tgattacaca tttccgtcaa
     6301 gctatagaag aaacgctgcc ctggctttcc tcttttggcg ctgacccagc gggtgggatg
     6361 acccgtttac tttattcgcc ggaatggctg gaaacccagc agcagtttaa aaaaagaatg
     6421 gcagcaagcg ggctggaaac acgtttcgat gaagtgggga atttatacgg tcgcctgaat
     6481 ggcaccgaat atccacagga agtggttctg agcggttcgc atatcgatac cgtggttaac
     6541 ggcggtaacc tcgacgggca attcggtgcg ctggcggcgt ggctggcaat tgactggctg
     6601 aaaacgcaat acggcgcgcc gttacgtacg gtcgaagtgg tggcgatggc agaagaagaa
     6661 ggcagccgct tcccgtatgt tttctggggc agtaaaaata tttttgggct ggcgaatcct
     6721 gacgacgtgc ggaatatctg tgatgccaaa ggaaatagtt ttgtcgatgc gatgaaggct
     6781 tgcggattta ctctgccgaa cgccccacta actccgcgtc aggatattaa agcctttgtc
     6841 gaactgcata tcgaacaggg ctgtgtgctg gaaagtaatg ggcagtcaat tggcgtggtg
     6901 aatgcaattg tcgggcaacg tcgctatacg gtgacgctga atggcgaatc aaaccatgca
     6961 ggcaccacgc cgatgggtta tcgtcgtgat acggtttacg ctttcagtcg catttgccat
     7021 cagtctgtcg aaaaagcgaa aaagatgggc gacccgctgg tgctgacctt tggcaaggtg
     7081 gagccgcgtc cgaatacggt gaatgtggta ccgggtaaaa ccacgttcac cattgattgt
     7141 cgtcataccg acgctgccgt gctgcgcgat ttcacccagc agttagaaaa cgacatgcgg
     7201 gcgatttgcg atgaaatgga tattggtatt gatatcgatt tatggatgga cgaagaaccc
     7261 gtgccgatga ataaggagct ggtcgccacc ctgacagaat tgtgtgaaag cgaaaaactg
     7321 aattaccggg tgatgcacag tggtgccggg cacgacgcgc aaattttcgc gcctcgcgtg
     7381 ccgacctgca tgatttttat ccccagcatc aacgggatca gccataaccc ggcggaacgc
     7441 accaatatta ccgaccttgc cgaaggggtc aaaacgttgg cactcatgct ttatcaactt
     7501 gcctggcaga aataaggagt tataaatggg atatttaaat aacgtcaccg gttaccgcga
     7561 tgatttactg gctaaccgtg cgattgttaa acacggtaat ttcgcactgt taacaccaga
     7621 cggtctggta aaaaatatta ttccgggctt tgtaaattgt gacgcgacaa tcctctccac
     7681 gccaaagctg ggtgcctctt ttgttgatta tctggtcaca ctgcatcaaa acggcggcaa
     7741 ccaacagggc ttcggtggcg aaggcattga aacgttcctg tatgtgatct ctggaaatat
     7801 cactgccaaa gccgaaggga aaacatttgc cttaagcgaa ggtggctatc tttattgccc
     7861 gccaggctcc ttaatgacgt ttgttaacgc ccaggccgaa gacagccaaa tctttttata
     7921 taagcgtcgc tatattccgg tagaaggtca tgcgccgtgg ctggtttctg gcaatgccag
     7981 cgaactggaa cgcattcatt atgaaggcat ggacgatgtt attctgctgg attttctgcc
     8041 caaagagtta ggttttgata tgaacatgca tatcctctct tttgcaccag gtgccagcca
     8101 cggttatatc gaaacccacg ttcaggaaca cggtgcctat attctttccg gtcagggggt
     8161 ttataacctc gacaataact ggatcccggt gaaaaaaggc gattacatct ttatgggcgc
     8221 ttattcttta caggctggtt atggcgtagg gcgtggtgaa gcgttcagct atatttactc
     8281 gaaagattgc aaccgcgacg tagagattta agtcatacca ctcgcctgat tatttttagc
     8341 tatgttggat tttgccacgg tttattccgt ggcttttttt gtagtcaatt caggtaaaaa
     8401 agatgcgata taaatcgcat ctgctttaaa ggtaatagtc agtttttaat tccctgacct
     8461 attttaatgg cgcaggcaat atttcgcgcg ctgttgaaga gattggtttc accgctggcg
     8521 agcacttcgg ctaaaggtgc cagacgtggc aaaatgctga ataccgcgtc aatgccgtac
     8581 tggtgcacca cttccacgcc atcacccaat acgccagcaa tcccaatcac cgggacatta
     8641 aactgcttcg ccaccgacgc cacgcccagc ggcgctttac cgcctgccgt ttgcgagtcg
     8701 atgcgccctt ccccggtgat caccagtgct gcaccctgca ccgcctgcgc aagattgacc
     8761 gcattcaaca caatctcaat acccggttta atatccgcat tgaggaatac cgccgccgca
     8821 atgcccatac cgcccgccgc gccgccaccc gccatctggc agacgttgat ttcagttagc
     8881 tgttgtaaaa cacgggcgta attttgcagc ccctgttcca gctcttcgac catctccggc
     8941 gttgcccctt tttgcgggcc aaacaccgcc gccgcgccgc gtgcccctac cagcgggtta
     9001 tcaacgtcac atgctacttc aatatgacag ttcgccagac gcggatcgca ttcattcatc
     9061 tcaatacttg ccacgcgcgc taaattgccg ccgttagccg ccagcggctg accgtcggca
     9121 tcaaggaaac gcacaccgag cgcctgcgcc atgcccatac cgccgtcgac cgtcgcactg
     9181 ccgccaatgc cgagaataat atggcgaatg ccattatcca gcgcatggag aattaactcc
     9241 cccgtaccaa aactggaggc cagcaacgga ttacgctttt caggcacgac cagcatcagg
     9301 ccacttgctg ctgccatctc aatcaccgcc gttttcccgt cgccggtaat gccataaaaa
     9361 gcattcactt tttcgcccat cggcccgcag acttcaagct tcacgatgtt accgtccgtc
     9421 gcggcgacca tcgcctccac cgtcccttcg ccgccatccg ctatcggcaa acagatatag
     9481 tgcgcatcgg gaaatatggt cgaaaacccg gctttaattg cctgacaaca tttttctgca
     9541 cttaagctct ctttaaaaga gtctggcgca atgacaatct tcattatcaa tcctttttca
     9601 acaaattatt cgatgcgttc ccacggaata agattatcca taagaacagc cagtaaaatt
     9661 ccgaccagta agccgttact gagcagagga cgaagcgtta atggaaggtc ttgcagatac
     9721 acaggcggta atgccattaa aaatatgccg acaaataacg gcaatgcgag tcgataaata
     9781 ttgcgagcgg taaacgttat ttgctggcta aacaccagcg cggaaaagag taaaggcaga
     9841 taagaaacca gcatgaccgc actactcact ggtaaaggga tactgcaaaa cagtcgcgtg
     9901 agtgccggaa ccagtgccac cagcaggcaa ataacgctgc cataaataaa cgaacgccgc
     9961 gagtaatcgc ctgtctgggt taataaacca atggatgaaa caaacggtga aaatggaatt
    10021 actgccagcg gcacggttat cagtgtcata aatccggtcg ccacaaagct gcgacgataa
    10081 cgcgtattcc ccgcgccctg ctgcggataa aaaacatccg tgccccgaat cgcaccgtag
    10141 gtattgctga tatttaccag acctgtaatc accgccgtca gaataatccc cggcgacaaa
    10201 gcaccgccac tgccgagcgg gaaccactgc cagtgcaact caccggagag cgaatgcgaa
    10261 gaaggaaagc aaaagtacca caacaaccag ccagttatcg tgccgaccag caggccgtaa
    10321 cgggcaaaac gttgtggcag gaaaataatc atcgccagta ccaggcacat caccgccacc
    10381 gagagcgcga acggcggtaa ttgaatttta aaattcgggg cggctatgcc aaacggcagc
    10441 ccgagcatac ctttgaaaaa gatagtggtc agttgcgcac ccagcatcaa cataaacaag
    10501 accatcaccg acggcgtaaa taaccgtgcc aggcgatggc ctaatccgct aaaaccaatc
    10561 aacatcgtca gcacgccgga gagcgcaatc cccaccgcca gactggtagc gatatcgttg
    10621 atcggtgtcc cgcgcgatgc ttcgccaagg gtgatagtca ggatggttcc ccaccacagg
    10681 ccgcccggcc cttccataat agcgcgacga tgaccgcaga acgcctgagc gaagcaggcc
    10741 agtgcggtag caagaaaagc atattgcgtg agcgtaagca ggctactttg cggcaactga
    10801 aaagcagaaa gtagcgtagg aggaaccaca accgtgttgc aaaaaataaa gaaaaaccac
    10861 tggaatcctg ataacaggct ttcgcggccc actgcaaaat tgaacataaa cacaccttaa
    10921 ccggagaatg cccgccacaa ggacgggcat tgcagggcca gattactgct gatgtttaag
    10981 gataaattga cctttcggcg caacagggaa gccctgttcg atgtcgtaaa tcacatcacc
    11041 acgtaagatg gttttcgtga tacgcgcgcc aatagtacgg ccaacatacg ggctgacttt
    11101 gtggcgatat tccagatcgt cattggtaag aacatagctg ctattcggct gaatgaagac
    11161 gaagtcggca tcttttcctg gggcgatacg gcctttttgc tgcagaccga aaatatctgc
    11221 tgcgttagtc gccattaatt tgccgaacat tggcagagac attccgcgtt tctgtaccgc
    11281 ttcatcgaac atcacgtcca tgcagctttg cagaccagcg ataccgcccc acgctttcat
    11341 gatgttaccg gctttcattt ccggcgggca tggagagtgg tcggaaacca ggcagtctat
    11401 ttcaccgtta aacagttttt cccacatgcc tttctggttt tccagatcgc ggatcggcgg
    11461 tgaacacttc gccagagtac cgatttcttc gaactgatcg gtatccagca caaagtaatg
    11521 cgggcaggat tcacaagtaa catcctgacc ttcctgacgt gcacgagtca cttcctcaac
    11581 accttccggg ctgctgacgt ggcaaacgtg cagacggcaa ccagcgactt tcgccaggta
    11641 cagtacgcgg cgaatcgctt ccacttcggt aaataccgga cgcgaagcca catagtcatg
    11701 ggcagttacg cgaccttcac gcttcgcttc ttcgcccagt gcgtcacaga tcagcgcgtt
    11761 ttcgcagtgc accagcactg gctggcccag ttcgcccagc ttctgcgcgc ctttgaagaa
    11821 ttgccagtca ttgacgtcac ggaagtcgtt gtcgataccg cgatcgccac aggtcgcaac
    11881 gaagcatttg aaaccgacaa cacccacttc atccaactca tgcagacggt caatgttgta
    11941 agacaccagg ccaccgagtt gtgccgcatc gatagtcagc tttcctttag cggcatcgaa
    12001 cttcagttca attgaggcgc ggtcaaccgt tgcaggcagc tggttgagcg gcatttcgat
    12061 catggtggtg ataccacctt ttgctgctgc gcgagtaccg gtttcataac cttcccagtg
    12121 gctgcgaccc ggttcagaaa tatgggtgtg cgcatcaacc atgcccggcg aaaccaccag
    12181 accagacgca tccataacgt cttttgcatc gcccagatcc tggccgatag cagcaatttt
    12241 tccgtcttta acggcgatat ctacaacgcg agcttcgttt tctaaaataa cggtgccgtt
    12301 tttaatgatt aaatcaaaag acataacaaa ctccttgtaa aatacagatt ggtttttaac
    12361 tcaatgttca atatcgtgat taattaacct atggtttttt gttctcctgt tttttctgct
    12421 gttgtacgtt tctttaataa ggcgtaggcc gcaaaggcga cgatgacgcc gacaaaccat
    12481 gaaacacgtg ataacggttc cataaaggga ataaacttac cgccaagaga taaaataacg
    12541 gccaccagag ttactgaaaa cgcggtgagg ttaaaaccgt tatcgtaata tttataatcg
    12601 ccaggtgcgg tgtacagttc atcaagatta atttgcccac gcatcaccac aaaataatgc
    12661 gccatcatca caccaattac cggaccaagc attccgccga taatatcgag gaacagataa
    12721 atactgtcct gattttccat taatttccac ggacagatca gcaggctgat aatactggca
    12781 atcagtacgc cgtttttata ggtcagtttc gtcggtgcaa tggcggcaat ctgataacca
    12841 gccggaataa tattaccggt ggcgttagta gagatagtcg tcatcagaat aaccagtacc
    12901 gcaaagaacg aggcgaacag gctgtcccaa cgctgaacaa tatccagcac gttccaggtg
    12961 tcagcgccgt aatgaatact ggctccggca ataatacata ccccggcgac cgcaaacaga
    13021 atataggcca caactaaacc cagcgtttgc cccagcgcct gctcacgaaa cgagtgggcg
    13081 ttttgcgtaa agtcggatgc gctcaccgcc ggtgccgccc agaccgcaac caccgcgttt
    13141 atcaccacca gaaacaggaa gccactgttt tctgctttct gaataccgcc cggaatgtag
    13201 tcaaagattg gaccgatccc gaccagcgaa atcgcccaaa tcgccatacc gccgaaaacg
    13261 atatagatgc acgggttaag aatggcagtg aatttattta aaacttttcc gccgccaaaa
    13321 ccaataccaa cgttaaccag ccagaagagt aagaaagtaa ttaatcccgg tagagaaagg
    13381 cctaacagcg tgaaatcgcc accgagagtc aaaaatcccg gccagatttt gccaatcaga
    13441 atcaagcagg ccagtgaccc cgcgtaacat tgcaggccaa accacataat ggcagcaata
    13501 ccgcctctta ataatccggg aaacagtgca ccacgtacgc cgtaagaagc acgcaggatc
    13561 atggcaaacg gtacgccgta tttactgccc gcagcaccgt ttaataccat taccgcggca
    13621 atgaaaaagg cactgaggat aattgccagc ataatactaa aggtagacaa gccgagaata
    13681 aaaaagccgc caaccatcac ataattggga acgttatgaa ccgaacccat ccataaggta
    13741 aaatagttaa atgttttcca ggtccgctga ctttgcgttt tcggcagtag atcttcgcta
    13801 tagccgcgtt gctggaatag ttttctctga tgttccataa atccctctta atttcacgta
    13861 tcgaataacg ttgttttaat ctagctatga ggaacaaata aaataaaaat attattttta
    13921 atactgaagc aacatctttg gctgctttct ttttttaata ttgctggccg attcattttc
    13981 tgatatcggc catttttatt gcgggtatca ggccagttta tggttagcca ttaattccag
    14041 cgcctgcact aatgcagagt gatccaactg gctgccaccg tttgccgcac aggtattaaa
    14101 caattcctgg caggtcgcgg tgtttggcag gttcagcgcc agtgctttcg cactttgcag
    14161 tgccaggttg agatctttct ggtgcagcgc aattttgaaa cctggattaa aggtgcgttt
    14221 aatcatgcgc tcaccgtgaa cttccagaat acgtgaggaa gcaaagccgc ccatcagcgc
    14281 ctagcgcaca cgtaccgggt ccgcaccggc ttttgaagca aacaacaagg cttcagaaac
    14341 cgcttcaata ttgagcgcca cgataatctg atttgccact ttgcaggttt gaccgtcgcc
    14401 gttaccgccc acgagagtga tatttttacc gagcagttca aacagcggtt ttacacgttc
    14461 aaatactgct tcatcaccgc caaccataat cgataacgtc ccttcacgcg cgccgatttc
    14521 accgccggag actggcgcat cgagataatc gccgcccagt tcattcacct gacgagcgaa
    14581 acgtttagtt tcaatcgggg aaatggagct catatcaaca atggttttgc ccttcagcga
    14641 ggctttggta caaccatttt caccaaacag aacgtcttca acctgtggcg tatccggcac
    14701 cataataaaa atgatgtccg atgcatctgt tacctggcgc gcagtttcaa cactaacggc
    14761 gcccagtgac agtaattcat cagcaaccgg accaatggtt gtgacatgca gttgatgacc
    14821 ggcacgcgcc agattaatgg ccatcggtgt acccataatg cctaagccaa taaatcccag
    14881 tttcataatt aacctctttt aaatttcgct tttcctgaat tcagacaaca cgatcccctg
    14941 ccaacttagc ggcctaaaaa agcattgtct gaatagcgtt acgttttaac ggtacgggtc
    15001 catccagcgt aaaccggctt ccgtggtggt ttggggttta tattcacagc caacccaacc
    15061 gttgtagtca gaattttcaa ttaccgtaaa gagatattcg taattaattt ctccggttcc
    15121 cggttcgccg cgatgcggat tatcagcaat ttgcaggtga ccaattttat cggcccactg
    15181 agtcatggta ttggttaatt caccttccat ccgctgcatg tgataaatgt catactgaat
    15241 tttcaaattg cagcaaccaa catcgtcaat cagtttcagt gcctgccggg ttccggtgag
    15301 atgaaaacca ggaatatcaa aatggttaat aggttcaatc agtaataaaa tatcttcttt
    15361 catcagcata ttcgcagcat aacgcaggtt ttctacaagc gttgtgtgaa tctgttcact
    15421 gctgaaacca gtcggtgttt taccgaccag acagttaatt tttttattac ccagcgcacg
    15481 agcataacga atcgctgctg ctacgccatc ccgaaactct tcttcacggc caggaataca
    15541 ggcaataccc cgctcgcccg ccgcccagtc acctgccggt aaattgtgca gcgtatgttc
    15601 aagtttatta ctcgccagca cctgttttaa ttcttcaatg tcgtagtcat aagggaacat
    15661 aaattcaacg ccgcgaaaac cacactgcgc agctttctca aaacgggcga gaaaatcata
    15721 ttcaccaaat aacatcgata aattagcaga gaaacgtaac attatttatc tcccttattc
    15781 atagtgcatg aaacaggttt cagtcggtgc atccgctgcg ttatcggcga tatcttcaaa
    15841 ttccatgacg ttatccagtt cactgcccat cgaaatatta gtcacacgct cgagaataac
    15901 ttccacgact accggtaccc gatattgcgc cattaagatt ttcgcctgtt caaaggctgg
    15961 tgcaatatct tccggtttga aaacgcgaat tgctttacaa cctaaacctt ccgctacttt
    16021 tacgtggtcg acgccataac cgttcacttc gctggagtta atattttcga acgcgagctg
    16081 tacgcagtaa tccatatcaa aggcacgttg cgactggcga atcagaccca gataggcgtt
    16141 attgaccagt acatggatgt acggaatgtt gaactgtgcg ccaacggcta attcttcaat
    16201 caggaactgg aagtcgaagt caccggaaat cgccaccaca ttgcgctctg gatcagcggc
    16261 acaaaccccc agcgccgccg gaatcgtcca gcccagcggg cctgcctgcc cacagttgat
    16321 ccagtggcgg tctttaaaga catgcagcat ttgtgccgca gcgatttgtg acaggccaat
    16381 ggtggtgaca taacaaacat cgcggccaaa ggctttgttc atctcttcat acacgcgctg
    16441 cggtttcacc ggcacgttgt cgaagtgggt tttgcgcagc aaagtgcgtt tgcgctgctg
    16501 gcagtcggcg acccactctt tacgacacgg cagacgaccg gctttttgca tctcctgcgc
    16561 cacttcaacc agcagcgtca gcgccgcttt agcatcagag acaatgccaa gatccggaca
    16621 cagcacgcgg ccaatttgcg tcggctcaat atcgatatga acgattttgc gcccttcggt
    16681 gtatttctct accgaaccgg tatgacggtt agcaaaacgg ttaccgatac caaacaccat
    16741 gtcggacgcc agcaacgttg cattaccgta acgatgcgcg gtttgcaggc ccaccatccc
    16801 ggccatcagt tcatgatcgt ccgggataca gccccagccc atcagcgttg ggatcaccgg
    16861 aatgctggtc agttcagcaa actgttgtaa cagtacagct gcgtcagcat taattacccc
    16921 gcctccggca acaatcaccg gacgttcggc ctggattaac atttctacag ctttttcgat
    16981 ctgcatacgg ctggcagcag gtttgtagac cggcagcggt tcgtacatat caggatcaaa
    17041 ctcgatttcc gcaacctgta cgtcgaacgg taaatccacc agtaccggac ccggtcgacc
    17101 agaacgcatc aggtgaaacg cctgttgcag cacacgaggc accagcgccg cttcacgaac
    17161 tgtaaccgcc attttgctga ccggtttagc aattgcttca atatcaacgg cctggaaatc
    17221 ttctttatgc agacgggcgc gcggagcctg gccggtaata cacagaatag gaatggaatc
    17281 agcagatgcg gaatagagcg cggtgatcat gtccgtgccc gcaggaccgg aagtccccag
    17341 acatacgccg atattccctg ccgttgcgcg ggtataacct tccgccatgt gcgaagcacc
    17401 ttccacatga cgcgccagaa tgtgacgaat accgccgtgc ttacgcatcg ctgagtagaa
    17461 cggattgatt gcagctcccg gaacgccgaa ggcggtggtg ataccttctt tctccagcac
    17521 atacattgcc gcgtcaacgg ctctcatttt tgccatttta ttcctacctc tattaattgg
    17581 aaaaattttc caactttcaa attttgtcaa aaaaacccgc tccgcagagc gggatacgac
    17641 agacactata gatgtgcttt cagtcccaac gccgtgctga tatcgcgggc ggtgtctctg
    17701 accagctcac cctgactgac aaaacgatct tctgtcagtc ttgatgaagg cccggagatg
    17761 gagatagcgg caacaacact acctacatca tcgtaaattg ctgaagctat gcaattcagg
    17821 ccgacaacat gctcttcttt atctacggta tagcccagtt cacgcgcttg ttccaggtcc
    17881 ttcagcaagg tgggcatatc cacaagtgtc gttggcgtaa actgctgcaa accggtttgc
    17941 agaatgatgc tcatcaactc ttcttccgcc agcggataaa gcagcgcctt gcccgctccg
    18001 gaagcatgca gcggcagacg actgcccagc ggcgcacaca tcctgaccat cgatttacac
    18061 tctaactgac caattaatac cgcttcattg ccgttacgga tcgcgacatt gaccgtttcg
    18121 ccggaaagta acatcaggcg gcgcataaac ggcccggcga cggagaggac atcgcggtta
    18181 tggatgtacg ctgcaccgac gttaaagaca cctaatccta tatgccacca gcctaactga
    18241 ttgtcctgat agacaaaatc cgctgcctgt aaaaccttca gcaagcgaaa ggtcgtggag
    18301 agcggcaaat ccagattgag agagatatcg ctaaccgacg aacttccccc acttttttcc
    18361 agatattgca gaatcgcaat tccccgctct aacgcctgtg cgcccttctg tgctacaggc
    18421 tccgcctgtc ctggcctgcc gcgccgtcta acttccgtca tacctacgtt aactccgacc
    18481 tacaaatgag cataaacaat gaacaattcg tttatcttat gcaagtcggt tgcaggcgtc
    18541 acgcaaaaca gagttcctgt tcaggaatac tttcaacatc acagttgtca ctgccgccgc
    18601 gatcgatggt cagaaaatcg gtgacgcgct gccaggcgaa aagtgggtga tgccagacgt
    18661 tacgatggta attcactccc tgttcgccat tggtaataaa cgcccgcagg gttgacaggt
    18721 ctggtttgtc gtcacctaac gccaccacca ccacaaacac ttcacctttc atcgggataa
    18781 acgcctgtgt acccagcgga tgacgttcga gttcgtgaat ggtcagcggc agattcgccg
    18841 gttgcgcgcg gttaatgctg ataagcgtac ggtcttgctc aagaatctca accagcgcca
    18901 aatcgtggta acgctccacc agtccattgt taatatggaa aaaatcccgt ttctgcgttt
    18961 cgattacgtc gccataagca ctaaaggctt cctgacttaa cggtaatacc tgaagtttca
    19021 tctttttgtc cttgtaacat aattccgttc aaccaccgcc gaccggtcag cttggtttca
    19081 ataacaggca atctaaaacc ttaagagaac aacatcaaca caaaagttgg aatatttttc
    19141 caaaactaga tttagtccac aaattgaaca gggttatcat gtaaaaatac tgataaatca
    19201 gaggataaga aaatcacgca acttgcggat ttaaaagggg atacctgaag atgttcgatc
    19261 cagaaacctt gcggactttc attgcggttg ctgaaacagg tagtttttca aaagcggcag
    19321 aacgattatg taaaaccacg gcgacgatca gttatcgcat taaacttctg gaagagaata
    19381 ccggagtggc gctgtttttc cgcacgactc gcagcgtgac gttgacggcg gctggcgagc
    19441 atctactttg tcaggccaga gactggctga gctggctgga aagtatgccc agcgagttac
    19501 aacaggtgaa tgatggcgtg gaacgccagg tgaatatcgt catcaacaac ctgctctata
    19561 acccccaggc cgttgccagg ctgctggcgt ggctaaatga acgttacccg tttacccaat
    19621 ttcacatctc ccgacaaatc tatatgggcg tctgggactc gctattgtac gaaggttttt
    19681 cactggctat cggcgtcacg ggaaccgagg cgctggcaaa tacctttagt cttgatccct
    19741 taggatcggt gcaatggcgc tttgtcatgg cggcggatca tccgctggcg aacgttgaag
    19801 agccgctaac agaagcacag ttgcggcgct ttccggcggt caatattgaa gacagcgccc
    19861 gcaccttaac caaacgcgtc gcctggcgat tgccggggca aaaagagatt attgttcccg
    19921 atatggaaac gaaaatcgcc gcccatctgg cgggcgttgg cattggtttt ttgccaaaat
    19981 cactttgcca gtcaatgctc gataatcaac aactggtcag ccgggtaatc ccaacgatgc
    20041 gccctccttc gccattgagt ctggcatggc gcaaatttgg cagcggcaaa gcggtagaag
    20101 atattgtgac cttgtttacc cagcgcaggc cggaaatcag cggattttta gaaattttcg
    20161 gcaacccacg cagttaaaaa tcaagtttcc ttgcgcgttg gcgcgtaaaa tagcgcgcaa
    20221 gctgatttga ggttcacttt ttactatgca agagagacac acggaacagg actatcgtgc
    20281 cctgctgatt gctgatacgc ccattattga tgttcgcgcc cccatcgagt ttgagcaagg
    20341 cgcaatgccc gccgctatca acctgccgtt aatgaataac gatgaacgcg ccgccgttgg
    20401 catctgctat aaacagcaag ggtcagacgc ggcgctggca ctggggcata aactggtggc
    20461 gggtgaaatt cgtcagcagc gcatggacgc ctggcgggca gcgtgcctgc aaaatccgca
    20521 cggtattctc tgctgcgccc gtggcggtca gcgctcacat attgtgcaaa gctggttgca
    20581 tgacgcgggg attgattatc cgctggtgga aggcggttat aaggcactgc gccagaccgc
    20641 gattcaggcg actattgaac tggcacaaaa accgatagtg ctgattggcg gttgtaccgg
    20701 cagcggtaaa acgctgttag tgcagcaaca gccgaacggt gttgatctgg aagggttggc
    20761 gcgtcatcgc ggttcggcgt ttggtcgcac cttacaacca caacttagcc aggcgagttt
    20821 tgaaaacatg ctggctgccg aaatgctaaa aaccgacgcc catcaggatt tgcgcctgtg
    20881 ggtgctggaa gatgaaagcc ggatgatcgg ttcgaatcac ctgccggaat gtctgcgcga
    20941 acgaatgact caggcgacga ttgcggtggt agaagatccg tttgagatcc gtcttgaacg
    21001 cctgaacgaa gaatatttct tgcgtatgca tcatgatttt acccacgctt atggcgacga
    21061 acagggctgg caggagtatt gcgaatacct gcatcacggg ctttcggcga ttaaacgtcg
    21121 gctgggacta caacgctata acgaactggc tgcaaggctg gatgcggcgc tgacaacgca
    21181 actcaccacc ggcagcaccg acggtcatct ggcctggctg gtgccgttac ttgaagaata
    21241 ttacgacccg atgtatcgct atcagcttga gaaaaaagcg gaaaaagttg tctttcgcgg
    21301 tgagtgggcg gaagtggcgg aatgggttaa gacttatgcc tgtggttatt aacccacaaa
    21361 aatgcttttc cgttcctctt taataaattg tgcaactggt taactaccag ttgccatagg
    21421 gatatttttt atctttgata aagttttttg tactttccgg ataagaatac ccatgtttat
    21481 aggcacttcg gccgttacac atttccattt tttcacctga aaccgtttct atcagaatct
    21541 gcttatccgt caggcgaata ttgggcttgt caggtttctc cagccacact cctattttcc
    21601 cttccggagc cagcccgatt aacagatacc gcagata
//
LOCUS       DAHHTG010000075        21360 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.075, whole genome shotgun sequence.
ACCESSION   DAHHTG010000075 DAHHTG010000000
VERSION     DAHHTG010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21360)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21360)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21360
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.075"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            309..521
                     /gene="hha"
                     /locus_tag="NBI74_002833"
     CDS             309..521
                     /gene="hha"
                     /locus_tag="NBI74_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000453334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin expression modulator Hha"
                     /protein_id="HCD1933132.1"
                     /translation="MEVKTKEDWLYQFRRCSSRETLEKVISHTRYKLTPAELETFNSA
                     VDHRLAELTMNKLYDRVPASVWKYVI"
     gene            1265..1834
                     /locus_tag="NBI74_002834"
     CDS             1265..1834
                     /locus_tag="NBI74_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus Gp2 family protein"
                     /protein_id="HCD1933133.1"
                     /translation="MTHEYNPFWQQRIRETVLRALDVHPRLTALRVDLRLPDVPAATD
                     AAVISRFINALKARINAYQKRKRREGKRVHPTTLHYAWAREFGELKGKKHYHLLLLVN
                     RDTWCRAGDYRAPRSLAGMIKQAWCSALGVDAGPYATLAHFPDTSAVWLARNDDTGFQ
                     QVLERADYLAKEHTKAHCTGERNFGCSRS"
     gene            2094..2495
                     /locus_tag="NBI74_002835"
     CDS             2094..2495
                     /locus_tag="NBI74_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004020115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1933134.1"
                     /translation="MYAKSFIALDGNGRLTGARTAQAAPYANYTCHLCGSALRYHPQY
                     DTELPWFEHTDDRLTEHGQQCPYVRPERREIQLIKRLQQFVPDALPVVRKASWHCRQC
                     HHDYYGEQYCTHCQTGGFSIPRTTQEEICEF"
     gene            2483..2917
                     /locus_tag="NBI74_002836"
     CDS             2483..2917
                     /locus_tag="NBI74_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001556209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HCD1933135.1"
                     /translation="MRILNCYMANDSKGHFVTAKEAAKHNRQDVLCCVSCGCPLTLQR
                     GNDGQPPWFEHDQMTVAEKILLRCTWLDPAEKEARRLHLQGMTVPDYTVKVRKWFCVM
                     CDEDYEGEKCCPRCGTGVYSRAWGRQEVPSEDARADNPLQRL"
     gene            3272..3652
                     /locus_tag="NBI74_002837"
     CDS             3272..3652
                     /locus_tag="NBI74_002837"
                     /inference="COORDINATES: protein motif:HMM:NF013677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence hypothetical
                     protein"
                     /protein_id="HCD1933136.1"
                     /translation="MQKNVTPGRRKGCPNYPPEFKQLLVAASCEPGISISKLALENGI
                     NANLLFKWRQQWREGKLLLPSSESPQLLPVTLDAAAEQPESLAEDPETLSISCEVTFR
                     HGTLRFNGNVSEKLLTLLIQELKR"
     gene            3649..3996
                     /gene="tnpB"
                     /locus_tag="NBI74_002838"
     CDS             3649..3996
                     /gene="tnpB"
                     /locus_tag="NBI74_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019104956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HCD1933137.1"
                     /translation="MIPLPSGTKIWLVAGITDMRNSFNGLAAKVQTALKDDPMSGHIF
                     IFRGRSGSQVKLLWSTGDGLCLLTKRLERGRFAWPSARDGKVFLTQAQLAMLLEGIDW
                     RQPKRLLTSLTML"
     gene            4036..5430
                     /locus_tag="NBI74_002839"
     CDS             4036..5430
                     /locus_tag="NBI74_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HCD1933138.1"
                     /translation="MRAYERHLSDDIFLLKQRLAEQEALIHALQEKLSNREREIDHLQ
                     AQLDKLRRMNFGSRSEKVSRRIAQMEADLNRLQKESDTLTGRVYDPAVQRPLRQTRTR
                     KPFPESLPRDEKRLLPAAPCCPNCGGSLSYLGEDTAEQLELMRSAFRVIRTVREKHAC
                     TQCDAIVQAPAPSRPIERGIAGPGLLARVLTSKYAEHTPLYCQSEIYGRQGVELSRSL
                     LSGWVDACCRLLSPLEEVLHGYVMTDGKLHADDTPVQVLLPGNKKTKTGRLWAYVRDD
                     RNAGSALAPAVWFAYSPDRKGIHPQTHLACFSGVLQADAYAGFNELYRNGGITEAACW
                     AHARRKIHDVHVRIPSALTEEALEQIGQLYAIEADIRGMPAEQRLAERQRKTKPLLKS
                     LESWLREKMKTLFFGSGHGGERGALLYSLIGTCKLNDVDPESYLRHVLGVIADWPVNR
                     VSELLPWRIALPAE"
     gene            complement(5669..7027)
                     /locus_tag="NBI74_002840"
     CDS             complement(5669..7027)
                     /locus_tag="NBI74_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000823236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase-like activity of phytase family
                     protein"
                     /protein_id="HCD1933139.1"
                     /translation="MKRKIIPVLIGCTLSFSALAAQPTAERYVVSFPEGTHVNYAGAF
                     ASAFPNGLPVGIGSGLLFTGKQGDALTFATITDRGPNADSPKEGKNETKIFVTPDFAP
                     LLMTIRVQNGKAEAIDPRPLHDDKGAINGLPLASDVIGSTNEVAFSDTLHRLKGDNRG
                     LDTEGITPDGKGGYWLCDEYGPFLINIDSKGKIQAIHGPQAAEGEKAIAGGLPNILKW
                     RQANRGFEGLTRMPDGRIIVAVQSTLDIDAKSKKKALFTRLVSFDPASGKTAMYGYPI
                     DSAAYSKNSDAKIGDIVALDNQHILLIEQGRDKNNRMRNLIYEVDLNKASDLSGFDKP
                     GEYPEFDDEKTLSQRGITLAQKTQVVDLRSLGWQQEKAEGLALIDSKTLAVANDNDFG
                     VKVAMQHPVEGKTFKDYRVNAEGKLTLDDKQVETTLRVKPLEKPESDSELWIVTLPEA
                     LK"
     gene            7309..7440
                     /locus_tag="NBI74_002841"
                     /pseudo
     CDS             7309..7440
                     /locus_tag="NBI74_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            7964..8190
                     /locus_tag="NBI74_002842"
                     /pseudo
     CDS             7964..8190
                     /locus_tag="NBI74_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229544.1"
                     /note="with IlvB catalyzes the formation of 2-acetolactate
                     from pyruvate, the small subunit is required for full
                     activity and valine sensitivity; E.coli produces 3
                     isoenzymes of acetolactate synthase which differ in
                     specificity to substrates, valine sensitivity and affinity
                     for cofactors; also known as acetolactate synthase small
                     subunit; frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
     gene            complement(8192..8974)
                     /locus_tag="NBI74_002843"
     CDS             complement(8192..8974)
                     /locus_tag="NBI74_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HCD1933140.1"
                     /translation="METKQKERIRRLMELLKKTDRIHLKDAARMLEVSVMTIRRDLHQ
                     EDEPLPLTLLGGYIVMVNKPAPSMPVIHDVPKNHRDDLPIAILAAGMVNENDLIFFDN
                     GQEIPLVISMIPDAITFTGICYSHRVFVALNEKPNVTAILCGGTYRARSDAFYDASNS
                     SPLDSLNPRKIFISASGVHNHFGVSWFNPEDLATKRKAMNRGLRKILLARHALFDEVA
                     SASLAPISAFDVLISDRPLPADYVTHCQNGSVKIITPDSEDE"
     gene            9280..10200
                     /gene="rbsK"
                     /locus_tag="NBI74_002844"
     CDS             9280..10200
                     /gene="rbsK"
                     /locus_tag="NBI74_002844"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HCD1933141.1"
                     /translation="MDIAVIGSNMVDLITYTNQMPKEGETLEAPAFKIGCGGKGANQA
                     VAAAKLNSKVLMLTKVGDDIFADNTIRNLESWGINTTYVEKVPCTSSGVAPIFVNANS
                     SNSILIIKGANKFLSPEDIDRAAEDLKKCKLIVLQLEVQLETVYHAIEFGKKNGIEVL
                     LNPAPALRELDMSYACKCDFFIPNETELEILTGMSVDTYDHIRLAARSLVDKGLNNII
                     VTMSEKGALWMTRDQEVHVPAFKVNAVDTSGAGDAFIGCFSHYYVQSGDVEAALKKAA
                     LFAAFSVTGKGTQSSYPSIEQFNEFLTLNE"
     gene            10228..11544
                     /gene="fucP"
                     /locus_tag="NBI74_002845"
     CDS             10228..11544
                     /gene="fucP"
                     /locus_tag="NBI74_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose:H+ symporter permease"
                     /protein_id="HCD1933142.1"
                     /translation="MNDKNIIQMPDGYLNKTPLFQFILLSCLFPLWGCAAALNDILIT
                     QFKSVFSLSNFASALVQSAFYGGYFLIAIPASLVIKKTSYKVAILIGLTLYIGGCTLF
                     FPASHMATYTMFLAAIFAIAIGLSFLETAANTYSSMIGPKAYATLRLNISQTFYPIGA
                     ASGILLGKYLVFSEGESLEKQMSGMNAEQIHNFKVLMLENTLEPYKYMIMILVVVMVL
                     FLLTRFPTCKVAQTSHYKRPSAMDTLRYLARNPRFRRGIVAQFLYVGMQVAVWSFTIR
                     LALELGDINERDASNFMVYSFACFFIGKFIANILMTRFNPEKVLILYSVIGALFLAYV
                     ALAPSFSAVYVAVLVSVLFGPCWATIYAGTLDTVDNEHTEMAGAVIVMAIVGAAVVPA
                     IQGYIADMFHSLQLSFLVSMLCFVYVGVYFWRESKVRTALAEVTAS"
     gene            11556..12569
                     /locus_tag="NBI74_002846"
     CDS             11556..12569
                     /locus_tag="NBI74_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020245959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HCD1933143.1"
                     /translation="MSTRINLWRALFGEKPRILLENSDFTVTSFRYDSGVEGLKIANS
                     RGHLIILPWMGQMIWDAQFDGHGLTMCNMFRQPKPATEVIETYGCFAFHSGLLANGCP
                     SAEDTHLLHGEMACAAMDEAWLELDGDMLRLNGRYEYVMGFGHHYLAQPTVVLHKSST
                     LFDIKMAVTNLASVDMPLQYMCHMNYAYIPNATFSQNIPDEILRLRESVPSHVNPTAQ
                     WLAFNQRIMQGEASLSTLSQPEFYDPEIVFFADKLDAYTDQPEFRMISPDGTTFVTRF
                     YSAELNYVTRWILYNGEQQVAAFALPATCRPEGYLAAQRNGTLIQVAPQQTRTFTVTT
                     GIE"
     gene            12991..13245
                     /locus_tag="NBI74_002847"
     CDS             12991..13245
                     /locus_tag="NBI74_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001373091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCD1933144.1"
                     /translation="MEQKTLSAEPRRSFSNEFKLQMVTLALQPRGSVARIAREHDIND
                     NLLFKWLRLSLIKGRISRRLPLTNSSGIGVELLPVEMTLG"
     gene            complement(13492..14748)
                     /locus_tag="NBI74_002848"
     CDS             complement(13492..14748)
                     /locus_tag="NBI74_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011815933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HCD1933145.1"
                     /translation="MDFFRFLMSDVLSEPAVLVGLIALIGLIAQKKPVTECIKGTVKT
                     IMGFVILGAGAGLVVSSLGDFANIFQHAFGIQGVVPNNEAIVSVAQKSFGKEMAMIMF
                     FAMVINIMIARFTPWKFIFLTGHHTLFMSMMVAVILATAGMTGITLIAVGSLVVGVAM
                     VFFPAIAHPYMKKVTGSDDVAIGHFSTLSYVLAGFIGSKFGNKEHSTEDMNVPKSLLF
                     LRDTPVAISFTMSIIFLVTCLFAGADAVKELSGGKNWFMFSIMQSITFAAGVYIILQG
                     VRMVIAEIVPAFKGISDKLVPNARPALDCPVVFPYAPNAVLVGFLSSFAAGLIGMFTL
                     YLLNMIVIIPGVVPHFFVGAAAGVFGNATGGRRGAILGAFAQGLLITFLPVFLLPVLG
                     DIGFANTTFSDADFGALGILLGIIVR"
     gene            complement(14761..15048)
                     /locus_tag="NBI74_002849"
     CDS             complement(14761..15048)
                     /locus_tag="NBI74_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004717867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HCD1933146.1"
                     /translation="MKITVVCGNGLGTSLMMEMSIKSILKDLSVSADVDHVDLGSAKG
                     TPSDIYVGTKDIAEQLVAQSVAGKIVALENMIDKKAMRERLSVVLNELGAL"
     gene            complement(15064..15507)
                     /locus_tag="NBI74_002850"
     CDS             complement(15064..15507)
                     /locus_tag="NBI74_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011192680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HCD1933147.1"
                     /translation="MLKNLLNTEVVQVVEQAKDWREAVAISCRPLIENGSIEPRYVDA
                     IYRSHDTIGPYYVVGPGIAMPHARPEEGANKLSLALTLIPSGVNFDADENDPVKLLIV
                     LAATDSTSHIEAISQLAKLFDNEKDIQAILMAKTTQDILSVIARY"
     gene            15778..16809
                     /locus_tag="NBI74_002851"
     CDS             15778..16809
                     /locus_tag="NBI74_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000416152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCD1933148.1"
                     /translation="MEKTRKRRGTGRVTLQEVANFAGVGTMTVSRALRTPEQVSDKLR
                     EKIEQAVEELGYIPNRTAGALASGHSDTVAVLVPSLTDKASSRFMQSLQQVLNKNEFQ
                     LLLGCHEYNQLKEAEILMTLLQGNPAALVIFGSQLADKTHQILEKTNIPTINVVGSPF
                     SPAQITIETAFFEASHKLTEHLLEQGYKNIGFIGAHMDNRLQRQQLNGWHKAMLSHYQ
                     NSDLVITMPDAASLQLGRYALNEILLRQPELDAVICSHEEIALGIMFECQRRLLKIPE
                     NIAVACLDGSDNCDQTHPTLTSIRIDYKKMGIETGKLLIGLLNNNHDESEESRIVQFN
                     YQIELRQST"
     gene            16937..17491
                     /locus_tag="NBI74_002852"
                     /pseudo
     CDS             16937..17491
                     /locus_tag="NBI74_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907654.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(17510..17999)
                     /locus_tag="NBI74_002853"
                     /pseudo
     CDS             complement(17510..17999)
                     /locus_tag="NBI74_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011787828.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            complement(18779..18943)
                     /locus_tag="NBI74_002854"
     CDS             complement(18779..18943)
                     /locus_tag="NBI74_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001215044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl-diphosphate delta-isomerase"
                     /protein_id="HCD1933149.1"
                     /translation="MRGTIDALDELFATLSYDKPRVIALHFKISEGYTLLMLITTFTS
                     CLILCKNYGW"
     gene            complement(19055..19744)
                     /locus_tag="NBI74_002855"
     CDS             complement(19055..19744)
                     /locus_tag="NBI74_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003825705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HCD1933150.1"
                     /translation="MKTVLDSLKGKLIVSCQALENEPLHSPFIMSRMALAAAQGGAAG
                     IRANSVADISAIKEIVSLPMIGIIKRDYPGSEVFITATLREVDELMTVEPEIIALDAT
                     ARPRPGGQTLEELVTQIRARYPSVLLMADIATVEEAVTAERLGFDCVGTTLYGYTAET
                     ARHVLAENDCGFLREVLAAVSVPVVAEGNVDTPERAARCLTLGAHTVVVGGAITRPQQ
                     ITTRFIAAIAS"
     gene            complement(19744..21300)
                     /locus_tag="NBI74_002856"
     CDS             complement(19744..21300)
                     /locus_tag="NBI74_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001817032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HCD1933151.1"
                     /translation="MMQIFSGASSGAWFEKAQRFGKSFMLPIAVLPAAGLLLGIGGAL
                     SNPNTLTAYPFLNVDWLQAIFTIMTSAGSIVFANLSVLFAVGVAVGLAKSDKGTAGLA
                     ALLAFLVMNATINALLTLNGTLAHKNPGSVGQGMTLGIQTLETGVFGGVVIGLVTCTL
                     HSRFNKVSLPQFLGFFSGSRFVPIISSLAAIVVGAVMTVVWPHFQKLIFGLGGLVDAT
                     GYLGTLLYGFILRMLGPLGLHHIFYLPFWTTALGGSEIVNGQLVEGTQRIFFAQLADP
                     NTQQFYAGTARFMSGRFITMMFGLLGACLAMYHTAKPENRKVVAGLLLSAALTSFLTG
                     ITEPVEFSFLFVAPVLYVIHAFFDGLAFMVAHILHITIGQTFSGGLIDFLLFGVLQGE
                     SKTNWMYVPIVGIPWFFLYYFTFRYLINRFGWLTPGRENVTLVESGQPQSERAAAVIA
                     GLGGKENLEEVDCCATRLRVTVKESSKVDEAALKVTGARGVIIRGNGVQVIYGPHVTI
                     IKNEVEEILS"
BASE COUNT         5520 a         5265 c         5225 g         5350 t
ORIGIN      
        1 ataaacactg tgagtgcgat cagtgggcat gagaacgcag tagcagcaga ttgcgttgta
       61 cccacagggc tatctctgat agctggcgta taacctgcgt atataaaacg tacctgcaac
      121 ccacgtgaac agagtgcatt aagcatccgt ctgagcggta ggcgtatccg acctggtgag
      181 tgacagtctg agagaccggt taagtggtgg cccgggagta atgagtttgg ggagggtacc
      241 agaatgccag tctggtgcgg tatgctgaca gtatcacgtt aattgtagca taaagtgatg
      301 agaggctaat ggaagttaaa accaaagagg actggctgta tcagttccgt cgttgttcat
      361 cccgggagac actggaaaaa gtgatttccc acacgcgtta taaacttacc cctgcggagc
      421 tggaaacctt caactctgcg gtcgatcacc gactggcaga actgacaatg aacaagcttt
      481 acgatcgtgt tcccgcttca gtctggaaat atgtcatctg agcgttcggt aatcacaacg
      541 gcatcgcgcc aggtgttatc cgctttgtga ttcttattgg ctcttctcag gttattaaaa
      601 gctgaatatg ttcctgttgt cgggaactag gataataccg ggaacgttaa gctgtataac
      661 gacaacaaag aattcgaaat caaaagggaa tggtgtccgg aggtgtgggt gatcatgggc
      721 tgattttcgg gtattaacgg cctgtatctg ctgaggtttt ctcgtattta ccggggatcc
      781 ggtgcgttac tgtctggcac agcaaacgca gaggcggcgg ggcgttctgc cccgttgatt
      841 ccggataatc cctcccctgt aacctactgc aacagtacaa atacactgac gcctgaaacg
      901 acagccagaa tcagcacagg cagtaacgtc agtagaaaga gcagacgatc ggtatttcgg
      961 taatagcctg aggaagctgt tgacgcgcag ctcgggcagc attccgtatt atgagtaatt
     1021 gcgttactac aatccctgca caccgttttg cgtatcacca taacgccctc ctcacagttt
     1081 tatccgtaac cggttttttg cattaaaaaa acaacgctga aaattcaacc gtcagtccgg
     1141 agacgacagt cggggtatca ctgagtgcct gagacattgc ccggttggag atcacttcag
     1201 ggactctgca cattttttac agcgtggtta ttccgtatga acgaaaccat acggagattt
     1261 aaccatgaca cacgagtaca accccttctg gcaacaacgt atccgtgaga cggtgctgcg
     1321 cgcactggat gttcatcccc gcctgacggc attgcgggtt gacctgcgtc tcccggatgt
     1381 accggcagca acggacgcag ctgtgatatc ccgcttcatc aatgccctga aagcccgaat
     1441 caacgcttac cagaagcgta agcgccgtga aggtaaacgc gtacatccca cgaccctgca
     1501 ttacgcctgg gcccgggaat tcggggagct taaaggcaaa aaacactatc acctcctgct
     1561 gctggtcaac cgggatacct ggtgtcgtgc cggcgattac cgcgctccga gatcactggc
     1621 cgggatgatt aaacaggcct ggtgcagtgc cctgggggtg gatgccgggc cttatgccac
     1681 gttggcgcat tttcctgaca cgtcggcggt gtggctggcg cgtaatgatg acaccggctt
     1741 tcagcaagtg ctggaacgtg ctgactatct ggcgaaggag cataccaaag ctcactgcac
     1801 cggtgagcgc aattttggct gtagccggag ttgagcacga ctggcgcgcc aatatccggc
     1861 aaaccaaaag tatgggttgt tgtctggcgg caacgatgta tccactcgcc cactgactgt
     1921 tttttacgtc agtgttccat tgtgtgacaa ccacagtgct gtaacagcaa ccagcctgct
     1981 ttacccaaac tccagtccag attttaactg aaccgccatt cacaccctga ccacgctgcc
     2041 cgtaccggga cggctgttgc ctgcgtgtcg tctggcggct gaggtatatg actatgtacg
     2101 caaaatcctt tatcgctctt gatggcaacg gacgtctgac gggcgcccgt actgcacagg
     2161 ccgcacctta tgctaactac acctgccact tgtgtggcag tgcactcaga taccatccgc
     2221 aatacgacac tgaacttccc tggtttgaac acactgacga caggctgaca gagcacggtc
     2281 aacagtgccc ttatgtcagg ccggagcgca gagaaataca gttgattaaa cgtctgcagc
     2341 aattcgtacc ggatgcctta cccgtggtgc gtaaagccag ctggcactgc agacaatgtc
     2401 accacgatta ttatggggag cagtactgca cacactgcca gaccggagga ttcagtattc
     2461 cccggacaac tcaggaggaa atatgcgaat tctgaactgt tatatggcga atgacagcaa
     2521 aggccatttt gttacggcga aagaagctgc gaagcacaac cgacaggacg ttttgtgctg
     2581 tgtgtcctgt ggatgcccgt taacacttca gcggggcaat gacggacaac caccgtggtt
     2641 tgaacatgac cagatgactg tcgctgaaaa aatcctgctg cgatgtacct ggcttgaccc
     2701 ggcagagaaa gaggcccgtc gtttgcatct gcagggcatg acggttccgg attatacggt
     2761 gaaggtgaga aagtggtttt gtgtgatgtg tgacgaagat tatgaggggg aaaagtgctg
     2821 cccacgctgc ggtaccgggg tatacagcag ggcgtggggg cggcaggagg tgccgtcgga
     2881 agatgccagg gctgataatc cgttacagag gctgtaatgg ttgcctccgg agcagtttgc
     2941 ggtgatgcct ttcccgggaa tggattgcca gtgcggggac tgtggagctt aattccggtg
     3001 ttggtggcct ttccgttgat tttatgccaa cagccccctg tgactgacag gctgcctcgt
     3061 cattccattc gtatctccag taacaggggg ttgtattgta ttttattgtg ccggttcagg
     3121 tgtgcgtttc ccggcgcgtc tgcaccggct taaccaaatt caacagggat aaaatagtgg
     3181 taagcgtaca gcctgaaccg tctggtcaga atctgacgaa ttagacaaag tggtgtccac
     3241 caaataagta gtgggaacca aagtgtcaga tatgcagaaa aatgtgactc ccggcaggcg
     3301 aaaaggctgc cctaattatc ctcccgaatt taaacagctg ctcgttgctg cctcctgtga
     3361 acccgggata tccatctcaa aacttgctct tgaaaatggc attaacgcca atctgttgtt
     3421 caaatggcga caacaatggc gcgagggaaa gctgctatta ccttcttcag agagccccca
     3481 gctacttcct gtgactctcg atgcagctgc cgaacagcca gaatcgctcg cagaggaccc
     3541 ggaaaccctc agtatcagct gtgaggtaac gttccggcac gggacgctcc gcttcaatgg
     3601 caatgtcagc gaaaagctcc tgactctgct gatacaggaa ctgaagcgat gatcccgtta
     3661 ccttccggga ccaaaatttg gctggttgcc ggtatcaccg atatgagaaa tagcttcaac
     3721 ggcctggctg cgaaagtaca aacggcgctg aaagacgatc ccatgtccgg ccatattttc
     3781 attttccggg gccgcagcgg cagtcaggtt aaactgctgt ggtccaccgg tgacggactg
     3841 tgcctcctga ccaaacggct ggagcgtggg cgctttgcct ggccgtcagc ccgtgatggc
     3901 aaagtgttcc ttacgcaggc gcagctggcg atgctgctgg aaggtatcga ctggcgacag
     3961 cccaagcggc tgctgacctc cctgaccatg ctgtaaatct ctttatcctg gttgtcacag
     4021 aataagcccg gtaaaatacg ggcttatgaa cgacatcttt ctgacgacat cttcctgctg
     4081 aaacagcgcc tggccgaaca ggaagcgctg atccacgccc tgcaggaaaa gctgagcaac
     4141 cgggagcgcg aaatagacca tctgcaggcg cagctggata aactccgccg gatgaacttc
     4201 ggcagtcgct ccgaaaaagt ctcccgccgt atcgcacaaa tggaagccga tctgaaccgg
     4261 cttcagaaag agagcgatac gctgactggt agggtgtatg acccggcagt acagcgtccg
     4321 ttgcgtcaga cccgcacccg taagccgttc cctgaatcac taccccgtga cgaaaagcga
     4381 ctgttgcctg cggcgccgtg ctgcccgaac tgcggcggtt cactgagcta tctgggcgag
     4441 gataccgccg aacagctgga gttgatgcgt agcgccttcc gggttatccg gacggtacgg
     4501 gaaaaacatg cctgtactca gtgcgatgcc atcgtgcagg cacctgcacc ttcgcggccc
     4561 atcgagcggg gtatcgccgg accggggctg ctggcccgcg tgctgacctc gaagtatgca
     4621 gagcacaccc cgctgtattg ccagtcagaa atatacggcc ggcaaggtgt ggagctgagc
     4681 cgttcactgc tgtcgggctg ggtggatgca tgctgccggc tgctgtctcc gctggaagag
     4741 gtgcttcatg gctatgtcat gactgacggc aaactccatg ccgatgatac cccggtccag
     4801 gtactgctgc cgggtaataa gaagacgaag accgggcggt tgtgggcgta tgttcgtgat
     4861 gaccgcaatg ccgggtcagc gttggcacct gcagtgtggt tcgcttacag cccggacaga
     4921 aaaggcatcc atccgcagac tcatcttgct tgcttcagcg gtgtgctgca agcggatgcg
     4981 tacgccgggt tcaacgagct gtatcgcaat ggtgggataa cggaagctgc ctgctgggct
     5041 catgcccgcc gaaagatcca cgatgtgcac gtccgcatcc cgtcagcact gacggaagaa
     5101 gccctagagc agatcggtca gttgtacgcc atagaggcgg atataagggg aatgccggca
     5161 gagcagcggc ttgctgaacg tcagcgaaaa acgaaaccgc tgttgaaatc cctggaaagc
     5221 tggttgcgtg aaaagatgaa gaccctgttc ttcggctctg gccatggtgg tgagcgggga
     5281 gcgctactgt acagcctgat cgggacgtgc aaactgaatg acgtggatcc agaaagctac
     5341 cttcgccatg tgcttggcgt catagcagac tggccggtca accgggtcag cgaactgctt
     5401 ccgtggcgca tagcactgcc agctgaataa cacatccccg tcaatacggc cctcgctgta
     5461 cgcttacttt tgttcggact gcttatggcc tttttccact ggtggcgcag gaaagttaac
     5521 cggctgccac catgggatga cgtgtaaacc gtgcaggcat gttttcacgt ggtgacgctt
     5581 tcattggggg tctacgccgg aaccccaata ttgttcacat acatgaattt gaaaaactaa
     5641 gcgatactgc tgtccgtacc ttataaactt atttcaacgc ttccggcagc gtcacaatcc
     5701 acagttcact gtcagactcc ggtttctcca gtggcttcac tctgagggtc gtttcaacct
     5761 gcttgtcgtc aagcgtcagc tttccttccg cgttcacccg ataatccttg aatgtcttgc
     5821 cctcgaccgg atgttgcatc gccaccttca cgccaaagtc gttatcgttc gccaccgcca
     5881 gcgttttgct gtcgatcagc gccaaccctt cagccttctc ctgctgccag cccaaggagc
     5941 gcagatcaac aacctgcgtt ttctgcgcaa gggtaatgcc gcgctgtgac agggttttct
     6001 catcatcaaa ctccggatac tcacccggct tgtcgaagcc tgacaggtcg ctcgccttat
     6061 tgagatcgac ctcgtaaatc aggttacgca ttctgttgtt tttatctctt ccctgttcga
     6121 tcagcaggat gtgctggtta tcgagcgcca cgatgtctcc gattttagcg tcgctgtttt
     6181 tgctgtaggc cgcgctgtcg ataggataac cgtacatcgc ggtttttccg cttgccggat
     6241 cgaagctcac cagacgcgta aacagcgcct ttttcttgct cttagcgtcg atatccagcg
     6301 tactttgcac ggcgacaata atacgcccgt ccggcatacg ggtgaggcct tcaaagccgc
     6361 ggttcgcctg acgccatttg aggatatttg gcaaaccgcc cgcaatcgct ttttcccctt
     6421 ccgccgcctg cggaccgtgg attgcctgga ttttcccttt actgtcgatg ttaatcagga
     6481 acgggccata ctcatcgcac agccagtaac ctcctttacc atccggagtg atcccttccg
     6541 tatccagccc gcgattatcc cccttcagtc tatgtaacgt gtcgctgaag gccacttcat
     6601 tggtggagcc aatcacgtcg cttgccagcg gcagaccgtt gatcgcgcct ttatcgtcat
     6661 gcagaggacg gggatcgatg gcctccgctt tgccgttttg tacacgaatc gtcatcagca
     6721 gcggcgcgaa atctggcgta acgaaaattt tagtttcgtt tttcccctct ttcggtgaat
     6781 ccgcgttcgg accgcgatcg gtaatggttg cgaatgtcag ggcatcgccc tgtttaccgg
     6841 tgaacagcag gccagaacct atcccaaccg gaaggccgtt cgggaatgca ctggcaaatg
     6901 cgccggcgta gttcacgtgc gtgccttctg gaaaactgac gacatagcgc tcagccgtag
     6961 gttgtgccgc cagggcagag aaagagagcg tacaaccgat tagcacggga ataatttttc
     7021 ttttcatatt gttagcatcc ataaagtgaa gatcacacta caccataaag aagaaacgag
     7081 tcaggaaaat gacatcgaat ggcaacatgc gcgggcctga ggccaaagaa atgtaaaaca
     7141 ctgttgttat ttgtaacaac ggaaacattt acggaaatta tttgtctttc ttcgtgatca
     7201 aaaatgctgt gtcttgttgg attggagtga tgagggctgc gtttaggaaa cgccctatat
     7261 tgtacggttg cattatcgcg gtaggggtaa tgcacgtgtc agtatgatga gctgaccaca
     7321 ctcacgtggg tggactggta taacaatcga cgattgctgg aaaggcaggg ccatatccca
     7381 ccggcagaag cagaaaaagc ttattatgct tccatcggaa acgatgatct ggcagcctga
     7441 gttcacagat aaaacactct ccaggaaacc cggggcggtt caattcgtaa ctggtagagt
     7501 tactacaaca agcatgggag gctctgactc tgacagacat tgtactgtgg aatgttgtta
     7561 caggttgggg cggcaaccgt gccaggagac catgagccgt tcacggcatt aaaggccaca
     7621 cttcagtcgt ttacattccc tatggtggca ggcatcttcc attaaggcgt cagagcgtta
     7681 ccaacgttat gttgtcatgg ctcatctttc atagtcgatg cttatctggt ggctgaagct
     7741 agccgcagat aaagagtgtg aagttgatac catggagtgg agtgacgtgc aggcaggaga
     7801 ttcgctctat gcagggttgt gagatgaatt caaaccggct ggcagcgcca gtgatattcg
     7861 aagattcgtc cggttgttac ccagtatgca ttaaaaaccc agacattatg gacaatataa
     7921 cggcttctac aacaaggaag tgcctgttta ccatccgggt gtaatgactc acctttgtgg
     7981 gcttttgccc gtcgcgctct taacgttgaa gacattctct gcctgccgat tcaggactgc
     8041 gacaaaagcc atatctggct actggtcaag gatgaccagc gtctggagta ggtgataagc
     8101 caaatagata agctggaata tgtggtgaaa gtgcaacgta atcagtccaa cccgacgatg
     8161 tttaacaaga ttgtggtgtt ttttcagtaa gtcattcgtc ttctgaatca ggtgtaatga
     8221 tctttacaga accattctgg cagtgcgtaa cataatctgc cggtaacgga cgatcgctaa
     8281 tcagaacgtc aaatgcagag atcggtgcga ggctggcaga ggccacttca tcgaacaacg
     8341 cgtggcgggc gagcaaaatt ttccgtagtc cacggttcat cgctttacgc ttagtggcaa
     8401 gatcttcagg gttaaaccag ctgacgccaa agtgattatg cacaccgctg gcggaaataa
     8461 atatttttcg cggattgaga gagtctaatg gcgaagagtt actggcatcg taaaaagcat
     8521 cacttctggc acgatacgta ccaccacaaa gtattgctgt tacattaggc ttttcattca
     8581 acgcaacaaa gacgcgatgt gagtaacaga tgccggtgaa ggtgattgca tccgggatca
     8641 tgcttataac gagtggtatc tcctggccat tatcaaagaa gatcagatca ttttcattaa
     8701 ccattccggc agccagaatt gcaataggta agtcatcacg atgatttttt ggaacgtcat
     8761 ggattactgg catggatggc gcgggtttat tcaccattac aatatagcca cccagtaggg
     8821 tcagtggcag aggttcatct tcctgatgga gatcgcgacg aatagtcatt acagaaactt
     8881 ccagcattcg cgctgcgtct ttcaaatgga ttctgtcggt tttcttaagc agttccatca
     8941 aacgtcggat acgctctttt tgcttcgttt ccatcaactc tctctctgta agaatgttat
     9001 aaaaataaca ttttggcgtt agtaaacatt gttttcatta ccatactata cgaccgcccc
     9061 tttgaaatga aaagggtata ggctgctgag ttttgggcac taatcttgtt aggttgctta
     9121 tgattagata gtgttggcct gagtgatgag actggaagtc tattaaataa acatcgggtt
     9181 gatgtgctaa tcgcgatctc agtcacaaat gatgcaaaag taacatgcta atgttattta
     9241 aataccattg ttatgcgaaa gtttataaga ggtagtgaaa tggacattgc agttattggt
     9301 tccaatatgg tggatctcat tacttacact aatcagatgc ctaaagaagg agaaactctg
     9361 gaagcacctg ccttcaaaat cggatgtggt gggaaaggtg ctaatcaagc ggtggcggcg
     9421 gctaaattga attcaaaagt tctgatgctg actaaagtag gtgatgatat ttttgcggat
     9481 aatactattc gtaatcttga atcatgggga attaatacca cttatgttga gaaggtcccc
     9541 tgtaccagca gtggtgttgc accgatcttt gttaatgcca actcgagcaa tagtatatta
     9601 ataattaagg gagcgaataa atttctttcc ccggaagata tcgatcgagc tgcagaagat
     9661 ctgaaaaaat gcaaattgat cgttttacaa ctggaagttc aactggaaac agtttaccac
     9721 gcgattgagt ttggtaagaa aaatggtatt gaagtgttat taaatccggc tccggcattg
     9781 cgtgagttag atatgtctta tgcctgcaaa tgtgatttct ttatcccgaa tgaaacagaa
     9841 ctggaaatat taactggtat gtcggttgat acttatgatc atattcgttt ggccgcacgt
     9901 tcgttagttg ataaagggct gaacaatatt attgttacca tgagcgaaaa aggcgcatta
     9961 tggatgaccc gcgatcagga agtccatgtt cctgcattca aagtaaatgc tgttgatact
    10021 agtggcgctg gtgacgcatt tattggttgc ttttcccatt attatgttca aagcggtgat
    10081 gttgaagccg ctttgaaaaa agccgctctc tttgccgctt tcagcgtcac agggaaaggt
    10141 acgcaatcat cttatccgag tattgaacaa tttaatgaat ttttaacctt aaacgaataa
    10201 tcatcctgca cagttaaagg tagcactatg aacgataaaa acatcattca gatgcctgat
    10261 gggtatctga ataagactcc cttgttccag tttattttgt tatcgtgttt attcccgcta
    10321 tggggatgcg cagctgcatt aaatgacata ttaatcactc aattcaaaag tgtattttcg
    10381 ctaagtaact ttgcttcagc attggtgcag agcgcatttt atggaggtta ttttttaatt
    10441 gcaataccag catcccttgt gattaaaaag accagttata aggtggcaat tttaattggc
    10501 ctgacgctgt atattggtgg ttgtacactt tttttccctg cctcgcatat ggcaacctac
    10561 actatgttcc ttgcagctat ttttgcgatt gcgattggcc tgagtttcct tgaaactgca
    10621 gcgaacacat acagttcaat gatcggtcca aaagcctatg ccacattgcg gctcaatatc
    10681 agccagacct tttatccgat tggtgctgcg tctggtattt tattgggcaa atatctggtt
    10741 ttctccgagg gggaaagcct tgagaagcag atgtccggaa tgaacgccga gcaaattcac
    10801 aactttaagg tgctgatgtt agagaacacc cttgaaccgt ataagtatat gattatgatt
    10861 ctggttgtgg tcatggtgtt gttcctttta acccgcttcc caacctgtaa agtggcacag
    10921 acaagtcact ataaacgccc gtcagcaatg gatacgctgc gctatctggc tagaaatccg
    10981 cgctttcgtc gcggtattgt ggcgcagttc ctttatgtgg gtatgcaggt cgcagtatgg
    11041 tcatttacca tccgtctggc gctggagttg ggcgatatca acgaacgtga tgcttcaaac
    11101 ttcatggtct atagctttgc ctgcttcttt attggcaagt ttatagccaa catccttatg
    11161 acgcgtttta atccggaaaa agtattgatt ctctattccg ttataggggc gctgttcctg
    11221 gcatatgttg cactggctcc aagtttcagc gcggtctatg tcgccgtcct ggtaagtgtt
    11281 ttgtttggcc cttgctgggc gacgatctat gcaggaacac tggatacagt tgataacgaa
    11341 catacagaaa tggcaggagc agtcattgtt atggctattg tcggtgccgc ggtggttccg
    11401 gcaattcagg gatatattgc agatatgttc cactcactac agctttcatt cctggtttct
    11461 atgctctgct ttgtctatgt cggagtctac ttctggcgtg aaagcaaagt acgtaccgca
    11521 ctggctgaag tcacagcgtc ctgaggagaa ccgttatgag tacgcgtatt aatttgtggc
    11581 gggcgctttt tggcgagaaa cctcgtatct tgctggaaaa tagtgatttt actgtcacgt
    11641 cttttcgcta tgacagcggt gtcgaggggc ttaaaattgc aaacagtcgt ggacatttga
    11701 ttattcttcc ctggatgggg cagatgattt gggatgcgca atttgatgga cacggcctca
    11761 ccatgtgcaa tatgtttcgc cagcctaaac cggcgacaga ggtgatagag acctatggct
    11821 gttttgcttt tcattcaggt ttgctggcta atggctgccc atctgcggaa gatactcatc
    11881 tgctacacgg tgaaatggcc tgtgccgcga tggatgaagc atggctggaa ttagacggag
    11941 atatgttacg ccttaacggg cgttacgagt atgtaatggg tttcggtcat cattatctgg
    12001 ctcaaccaac agtggtcctt cacaaatcct ctacactgtt cgatattaag atggccgtaa
    12061 ccaatctggc ttctgtagat atgccactac aatatatgtg ccacatgaat tatgcatata
    12121 taccaaatgc tacattcagc cagaatatcc cggacgaaat attaagattg cgtgaatcag
    12181 tgccttcgca tgttaatccc accgctcagt ggctggcgtt taatcaacgg attatgcagg
    12241 gagaagcctc tctgtcgacg ctgagtcaac cggaatttta tgatccagaa attgtcttct
    12301 ttgcagacaa actggacgct tatacagatc agccagagtt tcgcatgatc tctcccgatg
    12361 gcactacgtt tgttactcgt ttctacagtg ctgaactaaa ttatgtgaca cgctggatcc
    12421 tttataacgg agagcaacag gtagccgcct ttgcattacc tgcaacctgt cggcctgagg
    12481 ggtatcttgc cgcgcaacgt aatggcacgc tgattcaggt tgcaccgcag caaacgcgca
    12541 cttttacagt caccacagga attgaataac cactcaaggc ctgaacagcg gcgcgcttat
    12601 cgttaaggta agcgcgcttt tttacccgcc aggacaagac tatgatagcg ttgcccttat
    12661 agacgatcac attatcgtcc actccaaatt tgcgcaacta ctgtagagtg gtgtactcca
    12721 cagcttcttt gaggatctca acttccattg ttttcttacc gagcaagcac tgcaactctt
    12781 ttatttattt gtagtggtca acaaaaactg gtcacaattt cagttttttt ccagtatcta
    12841 ttttccgatt cgttcggcgg taatccaccg ttatattcat gaggccggag tgcactataa
    12901 aagtgtatgc gtaaaccgac tgctgtatgt agccattaag ccagtattgg taacataggt
    12961 acccaccttt tcaactagtg gacacctgtt atggaacaaa aaacattatc agctgaaccc
    13021 cgcaggtcat tttcaaatga gtttaaactt caaatggtta cactggcttt acaaccaagg
    13081 ggttctgttg cccgtattgc ccgggagcat gatatcaatg ataatcttct gttcaaatgg
    13141 ctcaggctct cgctgatcaa agggcgtata tcacggcgtc ttccgttaac gaactcttct
    13201 ggcattggcg ttgaattatt acctgtggag atgacgctgg gatgagcggc cttaccccgt
    13261 tttttacgcg aggtaattat gaagatcaat gactagccgt cgaaccgtgt gcatgaactg
    13321 ctgcactgaa acttctcagc tgtaaaataa tccttacgct acgtacttct cggagcgctt
    13381 acgattaatc ggatgcagtc ctgactggaa gaaagaaaaa tggagaaaat aagaggaatt
    13441 aatatcctcc acgttcctga aatcctggag gatattaatg tcatcaaaat attagcgaac
    13501 gataatccct aacaaaatac ccagtgcgcc aaagtcagca tcactgaagg tggtgttagc
    13561 aaaaccaata tcacccagca ccggcaataa gaatactggc aggaaggtaa taagcaagcc
    13621 ttgagcgaag gcacccaaaa tcgcaccacg acgtccaccc gttgcattac cgaatacccc
    13681 tgcagctgca cctacgaaga agtgcggcac cacaccagga ataatcacga tcatgttcag
    13741 cagatacaac gtgaacatac cgattaaacc tgcagcaaag ctactcagga aaccaaccag
    13801 tacagcgtta ggtgcgtaag ggaagacgac cgggcaatcg agagcaggtc tggcattcgg
    13861 aaccagcttg tctgaaatac ctttgaatgc cgggacaatc tccgcaatca ccatgcgcac
    13921 accctgcaga atgatgtaca cgccagctgc gaaggtaatg gattgcatga tagagaacat
    13981 gaaccagttt ttaccaccgc taagctcttt caccgcatct gcacccgcaa acaggcacgt
    14041 caccaggaaa attatgctca tggtaaaaga gatagcaacc ggcgtgtcgc gcaagaacag
    14101 cagacttttc ggcacattca tgtcttcagt cgagtgctct ttattaccaa acttgctgcc
    14161 gatgaaaccg gccaatacat aagataatgt tgagaagtgg ccgatggcaa cgtcatcaga
    14221 accggtcact ttcttcatgt acggatgcgc aatagccggg aaaaagacca ttgccacgcc
    14281 aacaaccaaa gagccaacgg ctataagcgt aatgccggtc atgcctgctg ttgccagaat
    14341 taccgctacc atcatcgaca taaacaacgt gtgatgaccg gtcaggaaga taaatttcca
    14401 cggtgtgaaa cgggcaatca taatgttgat aaccatcgca aagaacataa tcatcgccat
    14461 ttctttcccg aagcttttct gcgctacgga aacaatggct tcattgttcg gcactacacc
    14521 ctgaatacca aaggcatgct ggaaaatgtt tgcgaagtca cccagtgacg acacaactaa
    14581 acccgcccct gcgcctaaaa tcacaaaccc cataatggtc ttgacggtac ctttaatgca
    14641 ttcggttacc ggttttttct gtgcaatcag gccaatcagg gcgattaaac cgaccagcac
    14701 tgcgggttcg gaaagtacat cgctcatcaa aaaacgaaag aaatccatat cgcccccagc
    14761 ttacagtgcg cctaattcgt ttaataccac ggataaacgt tctctcatgg ctttcttatc
    14821 aatcatattt tccagtgcga cgattttgcc tgcgacagac tgcgcgacta actgctcggc
    14881 aatgtctttg gtgccgacat agatatcact cggcgtccct ttcgcggagc ccaggtcgac
    14941 gtgatcaaca tcagcgctga cggacaaatc cttcaaaatg cttttaatgc tcatttccat
    15001 catcaggctg gtgcctaagc cgttaccgca aaccactgta attttcataa atgttcctct
    15061 ggattaatag cgcgcaataa cagacaaaat gtcctgcgtc gttttggcca tcaaaatggc
    15121 ctgtatgtct ttttcgttat cgaataactt ggcgagttga gaaatcgctt caatgtggct
    15181 ggtactgtcg gttgcagcca gcacaatcag taatttcact ggatcgtttt catcggcatc
    15241 aaaattcaca cctgaaggga tcagggtcag tgctaatgac agcttgttag ctccctcttc
    15301 cgggcgcgca tgaggcatcg caataccagg gccaaccaca tagtacggac cgatagtgtc
    15361 gtgcgagcga tagatcgcat caacataacg cggttcgata gaaccgtttt caatcaacgg
    15421 ccggcatgaa atcgccacgg cttcccgcca gtctttggcc tgttccacga cctgaacgac
    15481 ctctgtattt aatagatttt ttaacatgct actgataact cccgatgata attaacagga
    15541 gagtagatat tttacgcatt aattaatgtg atgattgtcg catttgatag cgctatctga
    15601 taacgctacc aatctttgtg atagcgctat catttctctg ccgccatgct ttggctatat
    15661 tcggttcctg catatacaat gcaacaggat atttgctacc gtgggcaaaa actggaaaga
    15721 gtaatggtga aataacagta atgaaaacag ctggttgtaa ttatccatgg agtcgaaatg
    15781 gaaaaaacac gtaagcgccg tggtaccgga cgggtgacgc tgcaggaggt cgctaacttt
    15841 gcaggtgtcg gaaccatgac cgtttcccgt gcgctgagaa caccggaaca ggtatctgac
    15901 aagctgaggg aaaagataga acaggccgta gaagagttgg gttatattcc caacagaact
    15961 gctggcgccc ttgcatctgg tcacagtgat acagtcgccg tactggttcc ttccctgaca
    16021 gataaagcca gctcccgctt tatgcagtcg ttgcagcagg tgcttaataa aaatgaattc
    16081 cagctactgc ttgggtgtca tgagtataat cagctcaaag aagctgaaat tctgatgacg
    16141 cttcttcaag gcaacccggc ggcgttagtt atttttggtt ctcagttggc tgataaaact
    16201 caccagatac tcgagaaaac aaatatacct accattaacg ttgtcggttc ccctttcagt
    16261 ccggctcaaa ttaccatcga aacagcattt tttgaagcat cacataaact gaccgaacat
    16321 ctgctggaac agggttataa gaatatcgga tttatcggtg cgcatatgga caaccgcctg
    16381 caacgtcagc aactgaacgg ctggcataaa gcaatgttga gccattacca gaactctgat
    16441 cttgttatca ccatgccgga tgccgcaagt ctgcaattgg ggcgttacgc attaaacgag
    16501 atattactgc gacaaccaga gctggatgct gtgatttgca gccacgaaga gattgcgctg
    16561 ggtatcatgt ttgagtgcca gcggcgtttg ttaaaaattc ctgaaaatat cgctgtagct
    16621 tgtcttgatg gttcagacaa ttgcgatcaa acacacccaa ccttgacatc catccgtatt
    16681 gactacaaaa aaatggggat agagacgggt aagttactga ttgggctatt gaataataat
    16741 catgatgaat cagaagagtc gcgtatcgtg caattcaact accagataga attacgtcag
    16801 agtacatgag cctaaaaggg cactattaca aagttagcta ttgtggtagg gatagccgtt
    16861 tccggctatt ccccgcgcag atccggatgt atgctgctaa cgcatccatt cctacttcag
    16921 gtatttggcg tagaagcgga tgccaaaaac ttgccgcaaa accgcaatat cagcctgcag
    16981 agtcgctctc cagattatcc attcctatat tatctctgca ccggatgaat tacgcgaacc
    17041 actcagaaat atgacacgta tgcaacttat caggactccg ggatcctgga agcctagtgc
    17101 cagtgaatat cgtaatgtta ccaacgttta tcgcattgca ttgaaatcac ttgcctgacg
    17161 ctatttcgag ttacatgatg aaatcgccga ccttgatgtc atgattgcgg caattgtcga
    17221 tgaactggca cctgaactga ttaaacgtaa tgctgttgga tacgaaagcg cttcgcagtt
    17281 gctcatcact gccggagata atccccaacg gctaagaata gaatcaggtt ttgcggtgct
    17341 gtgtggtgtc aactcggtta ccgtatcttc taaaaaaatg aaccgttacc gacttaatcg
    17401 gggtggggag cgtgctgcaa atagtgcact tcacatcatc gctatcggac gtttacgaac
    17461 tgacgataaa acaaaggaat atgtcgctaa atagctgcgc ctaataccgc tacacttttg
    17521 ccagaccatg tctcccgccg gggaacggac tggcagtttc cataaagtgt tggacttttt
    17581 tcagcaactg ccacatctcc ggcactgatg attgcgcgtt atcgtttcat gaagcgcctg
    17641 ccatagccgt tcaaaatgat ttacccatgg cgagtaaact tgctgataaa tcaccctgaa
    17701 cttcggattt tccttcagcc aacgctgcgt ttcccggctt ttgtgaataa tgtaattatc
    17761 cacgatgagg ggcatcgttt ttgcccgccg gtacacagac ttcagttgct tcaacagact
    17821 gataaataag gctgaacttt gcggttactg cccacgtagc agactttgcc cgttcagcta
    17881 tgtaacgcgc cagcaagata atatttttca ttctgccccg gtgtcatcac ccgcttttgc
    17941 tgtccgcgct attgccagtc agcaccggtt ttgggatgga tagccaggtt aacagcgtcc
    18001 cgataacgtc ttgattctta taagggcgtt aactaatttc gttacatcat atacatgatg
    18061 cttgtgtgcg aagtaacgat agacaccaag tagatgctta aaggaagagg ctgacataaa
    18121 actcaaggat atgaactttg cctgcatata ggctacacca tggacgtaat agcgttaaaa
    18181 acgctactct tactaaacag attatacatc agatcgcctc gcagattacc tctatattcc
    18241 cagaagctcc gcccactgcc tgactcagga gtttctcgat aacgactatc cgctctatat
    18301 gccggataca cgcttgtatc acgtgggcct ggcgctgcaa aaaatggcgg ctaactatat
    18361 tgaaagcatc gatcaggccc gcgatttgcg gctattgatc tcatttctga gcaaacggtt
    18421 gggaaaacca gctacgcggc gattctggac gacaggcagg tggtttattt ggcaaaagcg
    18481 ttctcctatg atgaactctc tcattaatgc ggcatatcgg atcggcaata atctcccagc
    18541 cactgtaccg ccgcttgtaa agtgctgctg agccaatatc actttctgat aggcttctga
    18601 agcgtcatgg gcattcttac gcaataaccc gttataccac tgtgaccaca accagacggt
    18661 accgccgaag caattaaaca accaataatg gtgttgataa gacacggaag gatgtactgc
    18721 tcgccattca gccataacag ttgcagggta tatacagcag taatggtaaa accaaccttc
    18781 accagccgta gtttttgcac aatatcaggc agctggtgaa ggtggtaatc agcatcaaca
    18841 gggtataacc ttcggaaatt ttaaagtgca gcgcaatcac ccgcggtttg tcgtagctca
    18901 gcgtggcgaa tagttcgtcc agcgcgtcaa tagtccccct caccttgcag ggtctggaaa
    18961 atctagctaa tcgggtactg cataacactc tcaatatggg gataagcgat aattatcgct
    19021 tatccccacc aaagtatcat accccatcgg tgctttacga ggcgatcgcc gcgataaagc
    19081 gtgtcgttat ctgctgcgga cgagtgattg cccccccaac caccacggta tgagcgccta
    19141 atgtcaggca acgtgcggca cgttccgggg tatcgacgtt cccttccgcc acaaccggaa
    19201 cgctgaccgc tgccaggacc tcacgtaaga atccacagtc attttccgcc agcacatgcc
    19261 tggcagtttc tgcggtatag ccgtagagcg tagtgcccac acagtcaaaa cccagacgtt
    19321 cagctgtcac cgcctcttct actgttgcta tatctgccat cagaagtacg gacgggtagc
    19381 gagcgcggat ctgcgtgacc agttcttcca gcgtctgacc accagggcgc gggcgcgcag
    19441 tagcatccag ggcaataatt tccggctcaa cggtcatcaa ctcatccact tcccggagag
    19501 tggccgtgat aaacacctca ctgccagggt aatcgcgttt gataatccct atcatcggaa
    19561 gggagacaat ctctttgata gcactaatat ccgccacgct gttggcacgg atacctgcag
    19621 caccaccttg tgctgccgcc agcgccatac gtgacataat aaacggacta tgtagaggct
    19681 cattttccag agcctgacag gagacgatta atttaccttt cagggaatcc agtacagttt
    19741 tcattacgat aaaatctctt cgacttcgtt tttaatgatg gtgacatgag ggccataaat
    19801 cacctgaacg ccgttgccgc gaataatcac gccacgcgct ccggtcactt tcagcgcagc
    19861 ttcatctacc ttactgctct ctttaaccgt cacgcgcagg cgcgtggcgc aacagtccac
    19921 ctcttccaga ttctctttgc ctcccagccc ggcaatgact gccgctgcgc gttcactctg
    19981 aggctggccg gattcaacca gggttacgtt ctcacgaccc ggcgtcagcc agccaaagcg
    20041 gttaatcaaa tagcggaaag tgaagtagta aaggaagaac cacggtattc ccactatggg
    20101 gacatacatc cagtttgttt tagattcccc ctgcaacacg ccgaagagaa ggaaatcgat
    20161 aagcccgccc gaaaaggttt gcccaatagt gatatgtaaa atatgggcga ccataaacgc
    20221 cagaccatcg aagaaggcgt gaattacgta taatacgggc gcgacaaaca ggaaggagaa
    20281 ctcaacaggc tcagtgattc ccgtcaggaa agaggtcaag gctgccgaca aaagtagtcc
    20341 ggcgacaacc tttctattct ccggctttgc cgtatggtac attgccagac atgcgccaag
    20401 caggccaaac atcatggtga taaagcgccc ggacataaag cgagcggtac ccgcgtagaa
    20461 ctgctgcgta ttcggatcgg caagttgggc aaagaaaatc cgctgcgtac cttcaaccaa
    20521 ctggccgttg acgatttcac tgccgcccag cgccgtcgtc cagaacggca aataaaagat
    20581 atggtgtaaa cccagtggac caagcatacg caggatgaag ccgtacaaca gcgtccccag
    20641 atagcccgtc gcatccacca ggccgcccag tccaaaaatg agtttctgga aatgcggcca
    20701 caccaccgtc ataaccgcac ccactactat cgcggcaagg gagctgataa tgggtacaaa
    20761 acgagaacca ctaaaaaaac cgagaaactg aggtagtgag actttattga aacggctatg
    20821 taacgtgcag gtgacgagac caatcaccac cccaccaaac acaccagttt ccagtgtctg
    20881 aataccaagc gtcatcccct ggccaactga accggggttc ttgtgcgcca acgttccatt
    20941 tagcgttaat aaggcgttga tggtagcatt catcaccaga aacgctagca atgccgcaag
    21001 cccggcagtg cctttatccg atttcgccag ccccacggcg acgccgaccg caaagagtac
    21061 cgacaggttg gcaaacacaa tagaacctgc gctggtcatg attgtgaaga ttgcctgcag
    21121 ccagtcgaca ttcaagaatg ggtaggccgt caacgtattg ggattcgaca gagccccacc
    21181 aatccccaac aacaaccccg ctgcgggcaa taccgcaatc ggcagcatga acgatttgcc
    21241 gaaccgctgc gctttttcaa accatgcgcc tgacgaagcg ccgctaaata tttgcatcat
    21301 tttttcatct ccccgatcat tgataaaaat ttttaccata actaatttta aaaatgaaaa
//
LOCUS       DAHHTG010000076        20943 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.076, whole genome shotgun sequence.
ACCESSION   DAHHTG010000076 DAHHTG010000000
VERSION     DAHHTG010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20943)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20943)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20943
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.076"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            complement(<1..248)
                     /gene="chbB"
                     /locus_tag="NBI74_002857"
     CDS             complement(<1..248)
                     /gene="chbB"
                     /locus_tag="NBI74_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIB"
                     /protein_id="HCD1933152.1"
                     /translation="MEKKHIYLFCSAGMSTSLLVSKMRAQAEKYEVPVIIEAFPETLA
                     GEKGQNADVVFLGPQIAYMLPEIQRLLPNKPVEVIDSLL"
     gene            complement(547..885)
                     /gene="osmE"
                     /locus_tag="NBI74_002858"
     CDS             complement(547..885)
                     /gene="osmE"
                     /locus_tag="NBI74_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmE"
                     /protein_id="HCD1933153.1"
                     /translation="MNKNMAGILSAAAVLTMLAGCTAYDRTKDQFVQPVVKDVKKGMS
                     RAQVAQIAGKPSSEVSMIHARGTCQTYILGQRDGKAETYFVALDDTGHVINSGYQTCA
                     EYDTDPQATK"
     gene            1087..1914
                     /gene="nadE"
                     /locus_tag="NBI74_002859"
     CDS             1087..1914
                     /gene="nadE"
                     /locus_tag="NBI74_002859"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HCD1933154.1"
                     /translation="MTLQQQIIKALGAKPQINAEEEIRRSIDFLKSYLQTYPFIKSLV
                     LGISGGQDSTLAGKLCQMAINELRQETGNESLQFIAIRLPYGVQADEQDCQDAIAFIQ
                     PDRVLTVNIKGAVLASEQALREAGIELSDFVRGNEKARERMKAQYSIAGMTSGVVVGT
                     DHAAEAITGFFTKYGDGGTDINPLYRLNKRQGKQLLAALGCPEHLYKKAPTADLEDDR
                     PSLPDEVALGVTYDNIDDYLEGKNVPEQVARTIENWYLKTEHKRRPPITVFDDFWKK"
     gene            2144..3031
                     /gene="cho"
                     /locus_tag="NBI74_002860"
     CDS             2144..3031
                     /gene="cho"
                     /locus_tag="NBI74_002860"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease Cho"
                     /protein_id="HCD1933155.1"
                     /translation="MVRRLTSPRLEFEAAAIYEYPEHLRSFLNDLPTRPGVYLFHGES
                     DTMPLYIGKSVNIRSRVLSHLRTPDEAAMLRQSRRISWICTAGEIGALLLEARLIKEQ
                     QPLFNKRLRRNRQLCALQLNEKRVDVVYAKEVDFSRASNLFGLFANRRAALQALQTIA
                     DEQKLCYGLLGLEPLSRGRACFRSALKRCAGACCGKESHEEHALRLRQALERLRVVCW
                     PWQGAVALKEQHPEMTQYHIIQNWLWLGAVNSLKEATTLIRAPAGFDHDGYKILCKPL
                     LSGNYEITELDPVNDQQAS"
     gene            complement(2991..3566)
                     /gene="ves"
                     /locus_tag="NBI74_002861"
     CDS             complement(2991..3566)
                     /gene="ves"
                     /locus_tag="NBI74_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310475.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="environmental stress-induced protein Ves"
                     /protein_id="HCD1933156.1"
                     /translation="MEYFDMRKMSVNLWRNAAGETREICTFPPAKRDFYWRASITSIA
                     ANGEFSLFPGMERIVTLLEGGEMFLESADRFNHTLKPLQPFSFAADLVVKAKLTAGQM
                     SMDFNIMTRLDVCKAKVRIAERTFTTFGSRGGVVFVINGAWQLGDKLLTTDQGACWFD
                     GRHTLRLLQPQGKLLFSEINWLAGHSPDQVQ"
     gene            complement(3769..4254)
                     /gene="spy"
                     /locus_tag="NBI74_002862"
     CDS             complement(3769..4254)
                     /gene="spy"
                     /locus_tag="NBI74_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-independent periplasmic protein-refolding
                     chaperone"
                     /protein_id="HCD1933157.1"
                     /translation="MRKLTALFVASTLALGAANLAHAADTTTAAPADAKPMMHHKGKF
                     GPHEDMMFKDLNLTDAQKQQIREIMKGQRDQMKRPPLEERRAMHDIIASDTFDKAKAE
                     AQIAKMEEQRKANMLAHMETQNKIYNILTPEQKKQFNANFEKRLTERPAAKGKMPATA
                     E"
     gene            complement(4584..5552)
                     /gene="astE"
                     /locus_tag="NBI74_002863"
     CDS             complement(4584..5552)
                     /gene="astE"
                     /locus_tag="NBI74_002863"
                     /EC_number="3.5.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase"
                     /protein_id="HCD1933158.1"
                     /translation="MDNFLALTLTGKKPVITEREINGVRWRWLGDGVLELTPLTPPQG
                     ALVISAGIHGNETAPVEMLDALLGAISHGEIPLRWRLLVILGNPPALKQGKRYCHSDM
                     NRMFGGRWQLFAESGETCRARELEQCLEDFYDQGKESVRWHLDLHTAIRGSLHPRFGV
                     LPQRDIPWDEKFLTWLGAAGLEALVFHQEPGGTFTHFSARHFGALACTLELGKALPFG
                     QNDLRQFAVTASAIAALLSGESVGIVRTPPLRYRVVSQITRHSPSFEMHMANDTLNFM
                     PFEKGTLLAQDGEERFTVTHDVEYVLFPNPLVALGLRAGLMLEKIS"
     gene            complement(5545..6888)
                     /gene="astB"
                     /locus_tag="NBI74_002864"
     CDS             complement(5545..6888)
                     /gene="astB"
                     /locus_tag="NBI74_002864"
                     /EC_number="3.5.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-succinylarginine dihydrolase"
                     /protein_id="HCD1933159.1"
                     /translation="MNAWEVNFDGLVGLTHHYAGLSFGNEASTRHRFQVSNPRLAAKQ
                     GLLKMKKLADAGFPQAVIPPHERPFIPVLRQLGFSGSDEQVLEKVARQAPHWLSSVSS
                     ASPMWVANAATIAPSADTLDGKVHLTVANLNNKFHRSLEAPVTESLLKAIFNDEKKFS
                     VHSALPQVALLGDEGAANHNRLGGHYGEPGMQLFVYGREEGNDTRPSRYPARQTREAS
                     EAVARLNQVNPQQVIFAQQNPDVIDQGVFHNDVIAVSNRQVLFCHQQAFARQSQLLAN
                     LRARVSGFMAIEVPTAQVSVSDAVSTYLFNSQLLSRDDGSMMLVLPQECREHAGVWCY
                     LNELLAADNPISSLKVFDLRESMANGGGPACLRLRVVLTEEERRAVNPAVMMNDTLFN
                     ALNDWVDRYYRDRLTAADLADPQLLREGREALDTLTQLLDLGSVYPFQREGGGNG"
     gene            complement(6885..8363)
                     /gene="astD"
                     /locus_tag="NBI74_002865"
     CDS             complement(6885..8363)
                     /gene="astD"
                     /locus_tag="NBI74_002865"
                     /EC_number="1.2.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate-semialdehyde dehydrogenase"
                     /protein_id="HCD1933160.1"
                     /translation="MTLWINGDWVTGQGALRVKRNPVSGGVLWQGNDADAAQVEQACR
                     AARAAFPRWARLSFSDRQVRVERFAGLLESNKAELTAIIARETGKPRWEAATEVTAMI
                     NKIAISIKAYHVRTGEQRSEMPDGAASLRHRPHGVLAVFGPYNFPGHLPNGHIVPALL
                     AGNTIIFKPSELTPWSGEAVMRLWQQAGLLPGVLNLVQGGRETGQALSALEDLDGLLF
                     TGSANTGYQLHRQLSGQPEKILALEMGGNNPLIIDEVADIDAAVHLTIQSAFVTAGQR
                     CTCARRLLLKSGTQGDAFLARLVAVSQRLTPGTWDDEPQPFIGGLISEQAAQQVVTAW
                     QELEAMGGRPLLAPRLLQAGTSLLTPGIIEMTGVTGVPDEEVFGPLLRVWRYDNFDEA
                     IRMANNTRFGLSCGLVSPEREKFDQLLLEARAGIVNWNKPLTGAASTAPFGGIGASGN
                     HRPSAWYAADYCAWPMASLESDSLTLPATLNPGLDFSDEVVR"
     gene            complement(8360..9394)
                     /gene="astA"
                     /locus_tag="NBI74_002866"
     CDS             complement(8360..9394)
                     /gene="astA"
                     /locus_tag="NBI74_002866"
                     /EC_number="2.3.1.109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine N-succinyltransferase"
                     /protein_id="HCD1933161.1"
                     /translation="MMVIRPVERSDVSALMQLASKTGGGLTSLPANEATLSVRIERAI
                     KTWQGELPKSEQGYVFVLEDSESGTVAGICAIEVAVGLNDPWYNYRVGTLVHASKELN
                     VYNALPTLFLSNDHTGSSELCTLFLDPEWRKEGNGYLLSKSRFMFMAAFRDKFNDKVV
                     AEMRGVIDEHGYSPFWQSLGKRFFSMDFSRADFLCGTGQKAFIAELMPKHPIYTHFLS
                     QEAQDVIGQVHPQTAPARAVLEKEGFRYRNYIDIFDGGPTLECDIDRVRAIRKSRLVE
                     VAEGQPAQGDFPACLVANENYHHFRVVLVRTDPATERLILTAAQLDALKCHAGDRVRL
                     VRLCAEEKTA"
     gene            complement(9391..10611)
                     /gene="astC"
                     /locus_tag="NBI74_002867"
     CDS             complement(9391..10611)
                     /gene="astC"
                     /locus_tag="NBI74_002867"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.81"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylornithine/acetylornithine transaminase"
                     /protein_id="HCD1933162.1"
                     /translation="MSQPITRENFDEWMIPVYAPAPFIPVRGEGSRLWEQQGKEYIDF
                     AGGIAVNALGHAHPELREALNEQASKFWHTGNGYTNEPVLRLAKKLIDATFADRVFFC
                     NSGAEANEAALKLARKFAHDRYGSHKSGIVAFKNAFHGRTLFTVSAGGQPAYSQDFAP
                     LPPDIRHAAYNDINSASALIDDATCAVIVEPIQGEGGVVPASNAFLQGLRELCDRHNA
                     LLIFDEVQTGVGRTGELYAYMHYGVTPDLLTTAKALGGGFPVGALLATEECASVMTVG
                     THGTTYGGNPLASAVAGKVLDLINTPEMLNGVKQRHDWFVERLNSINHHYSLFSEVRG
                     LGLLIGCVLNADYAGQAKQLSQEAAKAGVMVLIAGGNVVRFAPALNVSEEEVTTGLER
                     FAAACEHFVSRGSS"
     gene            11057..11863
                     /gene="xthA"
                     /locus_tag="NBI74_002868"
     CDS             11057..11863
                     /gene="xthA"
                     /locus_tag="NBI74_002868"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HCD1933163.1"
                     /translation="MKFVSFNINGLRARPHQLEAIVEKHQPDVIGLQETKVHDDMFPL
                     EEVAKLGYNVFYHGQKGHYGVALLTKETPISVRRGFPGDDEEAQRRIIMAEIPSPLGN
                     ITVINGYFPQGESRDHPIKFPAKAQFYQNLQNYLETELKRENPVLIMGDMNISPGDLD
                     IGIGEENRKRWLRTGKCSFLPEEREWMERLMSWGLVDTFRHANPQTADRFSWFDYRSK
                     GFDDNRGLRIDLLLASQPLAECCVETGIDYEIRSMEKPSDHAPVWATFRR"
     gene            12030..12740
                     /locus_tag="NBI74_002869"
     CDS             12030..12740
                     /locus_tag="NBI74_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HCD1933164.1"
                     /translation="MNAERKFLFACLIFALVIYAIHAFGLFDLLTDLPHLQTLIRQSG
                     LFGYSLYILLFIIATLFLLPGSILVIAGGIVFGPLLGTLLSLIAATLASSCSFLLARW
                     LGRDLLLKYVGHSHTFQAIEKGIARNGIDFLILTRLIPLFPYNIQNYAYGLTTIAFWP
                     YTLISALTTLPGIVIYTVMASDLANEGITLRFILQLCLAGLALFILVQLAKLYARHKH
                     VDLSASRRSPLSHPKNEG"
     gene            12745..13422
                     /gene="ydjY"
                     /locus_tag="NBI74_002870"
     CDS             12745..13422
                     /gene="ydjY"
                     /locus_tag="NBI74_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdjY"
                     /protein_id="HCD1933165.1"
                     /translation="MLQHYSVSWKKGLAALCLLAVAGLSGCDQKENAAAKVEYDGLSN
                     SQPLRVDANNHTVTMLVQINGRFLTDDTRHGIVFKDGSNGHKSLFMGYATPKAFYEAL
                     KEAGGTPGENMTMDNKETTHVTGSKLDISVNWQGAAKAYSFDEVIVDSNGKKLDMRFG
                     GNLTAAEEKKTGCLVCLDSCPVGIVSNATYTYGAVEKRGEVKFKGNASVLPADNTLAT
                     VTFKITE"
     gene            13436..14143
                     /locus_tag="NBI74_002871"
     CDS             13436..14143
                     /locus_tag="NBI74_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HCD1933166.1"
                     /translation="MMKMQSRKIWYYRITLIILLFAVLLAWALLPGVHEFINRSVAAF
                     AAVDQQGIERFIQSYGALAAVVSFLLMILQAIAAPLPAFLITFANASLFGAFWGGLLS
                     WTSSMAGAALCFFIARVMGREVVEKLTGKTVLDSMDGFFTRYGKHTILVCRLLPFVPF
                     DPISYAAGLTSIRFRSFFIATGLGQLPATIVYSWAGSMLTGGTFWFVTGLFILFALTV
                     VIFMAKKIWLERQKRNA"
     gene            14143..14691
                     /locus_tag="NBI74_002872"
     CDS             14143..14691
                     /locus_tag="NBI74_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HCD1933167.1"
                     /translation="MGLPPLSKIPFILRPQAWLHRRHYGEVLSPIRWWGRIPFIFYLV
                     SMFVGWLERKRSPLDPVVRSLVSARIAQMCLCEFCVDITSMKVAERTGSTDKLLAVAD
                     WRQSPLFSDEERLALEYAEAASVTPPTVDDALRARLATHFDAQALTELTALIGLQNLS
                     ARFNSAMDIPAQGLCRIPEKRS"
     gene            14701..15867
                     /locus_tag="NBI74_002873"
     CDS             14701..15867
                     /locus_tag="NBI74_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCD1933168.1"
                     /translation="MRHCGWLLGLLSLFSLATHASDWQEIKNEAKGQTVWFNAWGGDT
                     AINRYLDWVSGEMKTHYAINLKIVRLADAADAVKRIQTEVAAGRKTGGSVDLLWVNGE
                     NFRTLKEANLLQTGWAETLPNWRYVDTQLPVREDFSVPTEGAESPWGGAQLTFIARRD
                     VTLQPPQTPQALLEFAKANPGTVTYPRPPDFTGTAFLEQLLIMLTPDPTALKEAPDDA
                     TFARVTAPLWQYLDALHPYLWREGKDFPPSPARMDALLKANTLRLSLTFNPAHAQQKI
                     ASGDLPASSYSFGFREGMIGNVHFVTIPANANASAAAKVVANFLLSPDAQLRKADPAV
                     WGDPSVLDPQKLPDGQRETLQSRMPQDLPPVLAEPHAGWVNALEQEWLHRYGTH"
     gene            15840..17375
                     /locus_tag="NBI74_002874"
     CDS             15840..17375
                     /locus_tag="NBI74_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCD1933169.1"
                     /translation="MATPLRYALIFLLWAMIAVIYAPLIPAALTLISPALSLTHWQAL
                     FADPQLPQALLATLVSTTIAAVGALLIALLVIVALWPGPKWQRMCARLPWLLAIPHVA
                     FATSALLLFADGGLLYDYFPYFTPPMDKLGIGLGLTLAVKESAFLLWILAAVLSEKRL
                     LQQLIVLDSLGYSRWQCLNWLLLPSVASALAMAMLAIVAWSLSVVDVAIILGPGNPPT
                     LAVISWQWLTQGDADQQTKGALASLLLMLLLAAYVLLGYLLWRGWRRTIPRVDGVRKP
                     ATPLLPSNTLASFLPLTGVLCVVLLAILADQSTINSQALINSLTMGLVAAFIALLLLL
                     LWLEWGPQRRQLWLWLPILLPALPLVAGQYTLALWLNLDGSWTAVVWGHLLWVMPWML
                     FILQPAWQRIDSRLILIAQTLGWSRAKIFFYVKCPLMLRPALIAFAVGFSVSIAQYMP
                     TLWLGAGRFPTLTTEAVALSSGGSNGILAAQALWQLLLPLIIFALTALVAKWVGYVRQ
                     GLR"
     gene            17375..18028
                     /locus_tag="NBI74_002875"
     CDS             17375..18028
                     /locus_tag="NBI74_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707358.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCD1933170.1"
                     /translation="MLCVKNVSLRLPESRLLTNVNFTVDKGDIVTLMGPSGCGKSTLF
                     SWMIGALAGQFSCTGELWLNEQRIDMLPTAQRQIGILFQDALLFDQFSVGQNLLLALP
                     ATLKGNARRNAVNDALERSGLDGAFHQDPATLSGGQRARVALLRALLAQPKALLLDEP
                     FSRLDVALRDNFRHWVFSEIRAMAIPVVQVTHDLQDVPPDSPVLDMAQWSENYNKLR"
     gene            18095..19402
                     /gene="ynjE"
                     /locus_tag="NBI74_002876"
     CDS             18095..19402
                     /gene="ynjE"
                     /locus_tag="NBI74_002876"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310490.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YnjE"
                     /protein_id="HCD1933171.1"
                     /translation="MKRVSQMTALAMALGLACASSWAAELAKPLTLDQLQQQNGKAID
                     TRPSAFYNGWPQTLNGPSGHEPAALNLSASWLDKMSTEQLNEWIKQHNLKADAPVALY
                     GNDKDVDAVKTRLQKAGFTHISILSDALSEPSRLQKLPHFEQLVYPQWLHDLQQGKEV
                     TAKPAGDWKVIEAAWGAPKFYLISHIPGADYIDTNEVESEPLWNKVSDEQLKAMLAKH
                     GIRHDTTVILYGRDVYAAARVAQIMLYAGVKDVRLLDGGWQTWSDAGLPVERGTPPKV
                     KAEPDFGVKIPAQPQLMLDMEQARGLLHRQDASLVSIRSWPEFIGTTSGYSYIKPKGE
                     IAGARWGHAGSDSTHMEDFHNPDGTMRSADDITAMWKAWNIKPDQQVSFYCGTGWRAS
                     ETFMYARAMGWNNVSVYDGGWYEWSSDPKNPVATGERGPDSSK"
     gene            complement(19411..20031)
                     /locus_tag="NBI74_002877"
     CDS             complement(19411..20031)
                     /locus_tag="NBI74_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HCD1933172.1"
                     /translation="MLDRHLHPRIKPLLHQCVRVLDKPGITPDGLTLVGFAIGVLALP
                     FLALGWYLAALVAILLNRLLDGLDGALARRRGLTDAGGFLDISLDFLFYALVPFGFIL
                     AAPEQNALAGGWLLFAFIGTGSSFLAFAALAAKHQIDNPGYAHKSFYYLGGLTEGSET
                     ILLFVLSCLFPACFPWFAWVFGALCWMTTFTRVWSGYLTLKSLQRQ"
     gene            20118..20525
                     /gene="nudG"
                     /locus_tag="NBI74_002878"
     CDS             20118..20525
                     /gene="nudG"
                     /locus_tag="NBI74_002878"
                     /EC_number="3.6.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP pyrophosphohydrolase"
                     /protein_id="HCD1933173.1"
                     /translation="MKMIEVVAAIIERDGKILLAQRPAQSDQAGLWEFAGGKIEPDES
                     QRQALVRELNEELGIEATVGDYVASHQREVSGRIIHLHAWHVPDFHGTLQAHEHQALV
                     WCSPEEALRYPLAPADIPLLEAFMASRAARPAD"
     gene            complement(20491..20763)
                     /locus_tag="NBI74_002879"
     CDS             complement(20491..20763)
                     /locus_tag="NBI74_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnjH family protein"
                     /protein_id="HCD1933174.1"
                     /translation="MSRALIAVVLAFPLITLANPHYHPDVEVNVPPEVFSSGGQSAQP
                     CTQCCVYQDQNYSEGAVIKAEGILLQCQRDDKTLSTNPLVWRRVKP"
BASE COUNT         4974 a         5593 c         5345 g         5031 t
ORIGIN      
        1 agcagcgagt caattacttc aaccggtttg ttgggtaaca aacgctggat ttcgggcaac
       61 atataagcaa tctgcggccc taagaacacg acatcggcat tctgaccttt ttcaccagcc
      121 agtgtttccg gaaatgcttc aataatgacc ggaacttcat atttttctgc ctgtgcgcgc
      181 atttttgata ccagtaaaga ggtagacatg cccgcagaac aaaacagata aatgtgtttc
      241 ttttccataa aactgccctc atcgacgatt atctgtcagc cagacactcc gcaagcctta
      301 acctgcttcc atgctctggg taacttgcga aaccaacatg actttttttg tggctgaaag
      361 gagtatacgg tataggcctg agcaatagta tttctttgac ctgataaatt gtctctcctt
      421 gacctgtggt tatgaccctc ttcacatttc gggcaaataa ttcgcgaaga taattaagaa
      481 cacaggccaa aaaaaaccgg cgcaatggcc ggtttccgtt gttactcaag ctttcaggcg
      541 aagtgattac ttcgtagcct gcggatcagt gtcgtattca gcacaggtct gataaccgga
      601 gttgatgaca tgtccggtat catctaacgc gacaaagtag gtttctgctt taccatcacg
      661 ttgacccagg atgtaggtct ggcaagtacc acgagcatgg atcatgctca cttcagacga
      721 aggtttaccc gcaatttgtg caacctgcgc ccggctcatg ccttttttga cgtctttcac
      781 cacaggctgt acaaactggt ctttggtacg atcataagcc gtacaacccg ccagcatggt
      841 taataccgcc gctgcactca gaattcctgc catattcttg ttcatattcc gtcctcttgt
      901 ttatcagcgt gttagataag cctggaatac attgggcgct ttttcaagcc cgtgaacgaa
      961 acggctccgc tttcagagga ttcctgtatg acgttttaac caccattcag cccgctgtcg
     1021 cttgtcgttt cagtagcaac gggttagctt taaggaagtt ttgtcttttc tgtctggagg
     1081 ggttcaatga cattgcaaca acaaataata aaggcgctgg gcgcaaaacc gcagattaat
     1141 gctgaagagg aaattcgtcg tagtatcgat tttctgaaaa gctacctgca aacttatccg
     1201 ttcattaaat cactggtgct cgggatcagc ggcggtcagg actccacgct tgccggtaag
     1261 ctgtgccaga tggcgattaa tgagctgcgc caggaaaccg gcaacgaatc actgcaattt
     1321 attgccatac gcctgcccta tggtgtccag gctgacgaac aagattgcca ggatgccatt
     1381 gcctttattc aaccggatcg cgtattaacc gttaatatca agggcgcggt actcgccagc
     1441 gaacaggcgc tgcgcgaagc gggcattgaa ctgagcgatt ttgtccgtgg caatgaaaaa
     1501 gcgcgtgagc ggatgaaagc acaatatagc attgcgggta tgaccagtgg tgtcgtggtg
     1561 ggcaccgatc atgcagcaga agctattacc ggattcttca ctaaatatgg tgacggcggt
     1621 acggacatta atccgctgta tcgtctcaac aaacgtcagg gtaaacagtt actggcggca
     1681 ttaggttgcc cggaacacct ttataagaaa gcgccaacgg ccgatcttga agatgatcgc
     1741 ccttctctac cagatgaagt ggcactcggc gtgacctatg acaatatcga cgactatctg
     1801 gaagggaaaa acgtacctga acaggtcgcc agaacaatag agaactggta tctgaaaacc
     1861 gaacataaac gccgtccgcc aattaccgtt ttcgatgatt tctggaaaaa gtaataattt
     1921 gcacctattg gattgtgcga aaaagagtaa tttgttcacg ccggatgcgg cgtgaacgcc
     1981 ttactcgacc tacaaaacta tgcaaattca atacattgca ggagtcgaat aggcctgaca
     2041 ggcgtagcac gtcagacggt gtaacctttg tcatcaaccc gcctcttttt taatcgctcc
     2101 ccgcctgtta cactggatag ataaccagca ttcggagtca acagtggtac ggcgtttaac
     2161 ttctccgcgg ctcgaatttg aagctgcggc aatttatgaa tatcccgaac atttacgttc
     2221 attccttaat gacttaccca cccgaccagg ggtgtatctg tttcatggcg aaagtgacac
     2281 catgccgctc tatatcggca aaagcgttaa tatccgcagt cgggtgcttt ctcatttacg
     2341 caccccggat gaagccgcca tgctgcgaca atcccgacgc atcagctgga tatgtaccgc
     2401 cggtgaaatc ggcgctctac tccttgaagc gcgattaatc aaagagcagc agccgctgtt
     2461 taataaacgc ttgcgccgca atcgccagct ctgcgccctg caattaaatg aaaagcgtgt
     2521 tgatgtggtg tatgccaaag aggtggattt ttcacgagcc tccaacctgt ttggcctgtt
     2581 tgccaatagg cgcgcagctt tgcaagcatt gcagaccatc gccgatgaac aaaaactttg
     2641 ttatggcctg ttgggactgg aaccgttaag ccgcggtcgt gcatgttttc gttctgcatt
     2701 aaaacgttgc gccggggcgt gttgcggtaa agagagccat gaggaacatg cgctacgctt
     2761 gcgccaggct ctggagcgtt tgcgggtggt gtgttggcct tggcaaggcg cggtggcatt
     2821 gaaagaacag cacccggaaa tgactcaata tcatattatt caaaactggc tgtggctggg
     2881 ggcggttaat tcgctgaaag aagcgacaac gttaattcgg gcacccgccg ggtttgatca
     2941 cgacggttat aaaattcttt gtaagccgct gctttccggt aactatgaaa ttactgaact
     3001 tgatccggtg aatgaccagc aagccagttg atttcactga acaacagttt accttgcggt
     3061 tgcaaaagcc gcagggtgtg cctgccatcg aaccagcacg cgccctggtc ggtagtgagc
     3121 agtttgtcgc ccaattgcca cgccccgttt attacaaaca ccaccccgcc gcgtgaacca
     3181 aaggtggtaa acgtacgttc ggcaattctg actttcgcct tacagacatc caggcgtgtc
     3241 atgatgttga aatccatcga catctgccct gccgtcagtt tcgcttttac caccagatcc
     3301 gctgcaaagg aaaaaggttg taacggtttt aaggtatggt taaagcggtc tgcgctttca
     3361 aggaacatct cgccgccttc cagcaacgtc actatccttt ccataccggg aaacaaagaa
     3421 aactccccat tcgccgcgat ggatgtaatg ctggcacgcc agtaaaaatc acgttttgcc
     3481 ggtgggaaag tgcaaatttc gcgcgtttca ccggcagcgt ttcgccacag attcaccgac
     3541 attttacgca tatcaaagta ttccataccc gctcccggcc cgttcttttc attgttatcg
     3601 ttcaggtaac cgtggatggt tatcggcaac tcgggtgatt tacgttagtg gcgatcaggc
     3661 tttttttgca tctgtttgag aagagaaaag aaaaccgccg atcctgtcca ccgcattact
     3721 gcaaggtagt ggacaagacc ggcggtctta cgttttttgg ctgaaagatt attcagcagt
     3781 tgcaggcatt ttaccttttg ccgctggacg ttctgtcaga cgcttctcaa aattagcatt
     3841 aaattgcttt ttctgttccg gcgtcaggat gttgtaaatc ttgttctggg tttccatgtg
     3901 cgccagcatg ttagctttgc gctgttcttc catttttgcg atctgcgctt cagcttttgc
     3961 tttatcgaag gtatcgctgg caatgatgtc atgcattgcg cggcgttctt ccagtggcgg
     4021 acgtttcatc tggtcacgct ggcctttcat gatttcgcgg atctgctgtt tctgcgcgtc
     4081 ggtcaggttc aggtctttga acatcatgtc ctcatgcgga ccgaacttgc ctttatggtg
     4141 catcatcggc ttcgcgtcag ccggtgctgc agtagtggtg tctgcggcat gggccaggtt
     4201 agccgcgcca agagccaggg tagaggcaac aaacagtgca gttaatttac gcatattcta
     4261 tatccttcct ttcagttatt tattacggct ttcttaagta gcgtgccgtg ttgacgagat
     4321 taactttact gactttagcg tcaattaatc agagcaacgg taaaacaatg aaagtgtaaa
     4381 aaacactttt gcgccaatta tggagaaaaa aagaaaattt gatggagagt gatgagagaa
     4441 tattacaaca cgatgatttt gcagagatta tgaagaacta taccggatga ctggtgataa
     4501 ataaagcaaa taaccaggat taatctgtat taatttataa gaaagcaact taatacccgc
     4561 agaatgattt ctgcgggtaa gtattagctt attttttcga gcattaatcc cgcacgtaat
     4621 cccaacgcta ccaacggatt agggaataac acatactcta catcatgggt tacggtaaaa
     4681 cgttcctccc cgtcctgcgc cagcaatgtt cctttctcaa acggcataaa attcagcgtg
     4741 tcatttgcca tatgcatttc gaaggacggc gagtggcgag taatttgcga aaccacccga
     4801 taacggagcg gcggtgttct cacgataccg acactctcac cagatagcag cgcagcaatt
     4861 gcgctggcag ttactgcaaa ctggcgaaga tcgttttgtc caaagggcaa cgctttgccg
     4921 agttccagcg tacaggccag cgcgccaaaa tgtctggcgc tgaaatgggt aaacgtacca
     4981 ccaggctcct gatggaacac cagtgcctcc agacccgccg cacccagcca cgtcagaaat
     5041 ttctcgtccc agggaatgtc gcgttgcggt aatacaccga accgcggatg caaggagcca
     5101 cgaattgcgg tatggagatc aaggtgccag cgcacagatt ctttgccctg gtcataaaaa
     5161 tcttccaggc actgttccag ttcgcgcgcc cgacaggttt ccccgctttc agcaaatagc
     5221 tgccaacgac cgccaaacat tcggttcata tcgctatggc aataacgttt cccttgcttc
     5281 agcgcaggag gattcccgag gatcaccagc aaccgccaac gtaacgggat ctcgccgtga
     5341 gatatcgcgc caagcaacgc atccagcatt tccacaggtg ccgtctcatt accgtgtatt
     5401 cccgctgaaa tcaccagtgc gccttgcggt ggcgttaatg gcgtcagttc cagcacgcca
     5461 tcgcccagcc agcgccagcg aacgccgttg atttctcgct cggtgataac cggtttttta
     5521 cccgttaagg tcagagcaag aaaattatcc atttccccct ccctcgcgct ggaatggata
     5581 aaccgaacca agatccaata attgagtcag tgtatccagc gcttcccgtc cttcgcgcag
     5641 caattgtgga tcggcaagat ccgccgcagt aagacgatcg cggtagtaac gatccaccca
     5701 gtcattgagc gcattaaaca gcgtatcgtt catcatcacc gccggattca ccgcccggcg
     5761 ttcttcttct gtcaacacca cgcgcagccg caggcacgcc gggccaccgc cattcgccat
     5821 gctttcacgt aaatcaaaaa cttttagtga gcttattggg ttatcggcag caagcaattc
     5881 attgagataa caccatactc cagcatgttc ccgacattcc tgaggcagca ccaacatcat
     5941 ggaaccatca tcgcggctca gcagttggct gttaaacaga taagtagaca ccgcatctga
     6001 cacggaaacc tgcgccgtgg gcacctctat tgccataaaa ccactgaccc gtgcacgcag
     6061 atttgccagt aactgtgact ggcgagcgaa cgcctgttga tggcaaaaca gcacctggcg
     6121 gttactcacg gcaatcacgt cattatgaaa aacgccctgg tcgataacgt ccgggttttg
     6181 ctgggcgaaa atcacctgtt ggggattcac ctgattcaac cttgccaccg cctcgctggc
     6241 ttcacgggtc tgccgcgccg gataacggga aggccgggta tcattgccct cttctcgccc
     6301 gtagacaaaa agttgcatac ccggttcacc gtaatgaccg ccgagacgat tgtggtttgc
     6361 cgccccctca tcaccgagca aggctacctg cggcaacgcc gaatggacgc taaatttctt
     6421 ttcgtcgtta aaaatcgctt ttaacagcga ttcggtgacg ggggcttcca gcgaacggtg
     6481 aaatttattg ttcaggttag caacagtgag atgcactttg ccatccagcg tatcggcgga
     6541 tggcgcgatc gttgccgcat tggctaccca cattggcgaa gcggaactga cgctggaaag
     6601 ccagtgcggt gcctggcgtg caactttttc cagtacctgc tcatcgctac cactgaatcc
     6661 caactgacgc agcaccggaa taaacggacg ctcgtgcggc gggatcacgg cctgggggaa
     6721 tcccgcatcg gcaagttttt tcattttcag taagccctgt tttgccgcca gtcgcgggtt
     6781 agacacctga aaacggtgac gggtagaggc ttcattacca aacgacaggc ccgcgtaatg
     6841 gtgcgtcagc cctaccagcc cgtcgaaatt gacttcccag gcgttcatcg caccacctca
     6901 tcggaaaaat ccagcccggg gttaagcgtt gcgggcaacg ttaacgagtc cgactccaga
     6961 cttgccatcg gccaggcaca ataatctgcg gcataccagg cgctggggcg atgattgccg
     7021 gaagcgccaa tgccgccgaa tggcgcggta ctggcagcac cggtaagcgg tttgttccag
     7081 ttaacaatcc ccgcccgtgc ctccagcaac agttgatcga atttttcccg ctcgggggaa
     7141 accagaccgc aagagaggcc gaagcgagtg ttattcgcca ttcgaatcgc ttcatcgaaa
     7201 ttgtcataac gccagacgcg caataacggc ccaaacactt cttcatccgg cacgccagta
     7261 acgcctgtca tttcaatgat ccccggcgtc agcaacgatg tccctgcttg taataagcgc
     7321 ggcgcaagca ggggccgtcc gcccatcgcc tccagttcct gccaggcagt aaccacctgc
     7381 tgtgcggcct gctcagaaat cagcccgcca ataaacggct gcggttcgtc atcccaagtg
     7441 cccggcgtta accgctggct gacggcaacc aggcgagcaa gaaatgcatc gccctgcgtt
     7501 ccacttttca gcaataaacg gcgggcgcag gtgcagcgtt gcccggcagt gacaaacgcc
     7561 gactgaatgg tcagatggac agcggcatcg atatccgcca cctcatcgat aattagcggg
     7621 ttattaccgc ccatctcaag ggcgagaatt ttctccggct gaccggaaag ctggcgatgc
     7681 agctggtagc cggtattggc gctgccggta aacagcaagc cgtcgagatc ctccagcgca
     7741 ctcagcgcct gacccgtttc acgcccgccc tgcaccaggt tcagcacgcc cggcagcaag
     7801 ccagcctgct gccataaacg cattaccgct tcgccactcc acggtgtcag ttcgctgggt
     7861 ttaaaaataa tggtgttacc tgccagcaat gccggaacaa tatgtccgtt cggcagatga
     7921 ccagggaaat tatacggtcc aaacactgcc agtacgccgt gcggacgatg gcgcaagctc
     7981 gccgcgccgt ccggcatttc gctacgctgc tcaccggtac gaacgtgata cgccttaatt
     8041 gatatcgcga ttttattgat catcgccgtc acttcggttg ccgcttccca gcgcggcttg
     8101 ccggtttctc tggcaataat cgcggttaat tcagctttat tgctttccag caatccggca
     8161 aagcgttcaa ctcttacctg acgatcgcta aatgagagcc tcgcccagcg cggaaacgcc
     8221 gctcgggcag cccgacaagc ctgctcgacc tgagcagcat cggcatcatt gccctgccat
     8281 aacaccccgc ccgataccgg attacgcttc acacgcaatg cgccctggcc cgttacccag
     8341 tcaccgttaa tccataaagt catgctgttt tctcctctgc gcacaggcgc accagacgaa
     8401 cgcgatcccc tgcgtggcat ttgagggcat ccagttgtgc ggcggttaaa atcaaacgct
     8461 cggtcgctgg atcggtacgc accagcacca cgcggaaatg gtgataattt tcattggcga
     8521 ccaggcaggc cggaaaatcg ccctgcgcag gctgcccttc ggccacttcc accagccgac
     8581 ttttacggat ggcgcgcacg cggtcgatat cacactcaag tgtcggcccg ccgtcaaaga
     8641 tgtcgatata gttacggtag cgaaaacctt ctttctccag caccgcacgg gcaggtgcgg
     8701 tttgcggatg tacctgaccg atgacgtcct gtgcttcctg ggataaaaag tgcgtataga
     8761 tcggatgttt cggcatcagt tctgcaataa atgccttttg cccggtgccg cagagaaaat
     8821 cagcgcggct aaaatccatc gaaaagaagc gtttaccgag gctttgccag aacggtgaat
     8881 agccgtgttc gtcaatcacc ccgcgcattt cggcgaccac tttgtcatta aacttgtcgc
     8941 gaaaagccgc cataaacata aagcgcgatt tcgacagcaa atagccgttg ccctctttgc
     9001 gccactccgg gtcgagaaac agcgtgcaca gctcgctgct gccggtgtga tcgttactga
     9061 gaaacagcgt cggcaatgcg ttatagacat tcagctcttt cgaagcgtga accagcgtgc
     9121 cgacgcgata gttgtaccag ggatcgttca gcccaaccgc cacctcaatg gcgcaaatcc
     9181 ccgccacagt gcctgactcg ctatcttcca gtacaaacac ataaccctgc tcacttttgg
     9241 gcagttcgcc ttgccaggtt ttgattgccc tttcaatacg caccgaaagc gtggcttcat
     9301 tggcgggaag cgacgtcagg ccgccgcccg ttttgctggc aagctgcatc agcgccgaga
     9361 catctgagcg ctcaacggga cgaatgacca tcatgatgaa cctcggctaa caaagtgttc
     9421 gcaagcagct gcaaagcgct ccagtccggt cgtcacctct tcttcgctga cattgagcgc
     9481 tggcgcaaaa cgcaccacgt tgccacccgc aatcagcacc atcacgcctg ctttcgccgc
     9541 ttcctgagag agctgtttcg cttgcccggc gtaatcggca ttcagtacac agccaatcaa
     9601 aagacctaag ccgcgaactt cactgaacaa actgtagtgg tgattaatgg aatttagacg
     9661 ctcaacaaac cagtcgtgac gctgtttaac cccgttaagc atctctggcg tgttgatgag
     9721 atccagcact ttgcctgcca ccgccgaggc cagcggattg ccgccatagg tggtgccgtg
     9781 agtgccaacg gtcatcacgc tggcgcactc ttcggttgcc aacaacgcac caaccgggaa
     9841 accgccgccc agcgctttgg cggtagttaa cagatcaggc gtcacgccgt agtgcatata
     9901 ggcatacagt tccccggtgc gcccgacacc ggtttgtact tcatcaaaga taagcaacgc
     9961 attgtggcgg tcacacaatt cacgcagacc ttgtaaaaat gcgttgctgg ctggcaccac
    10021 accgccttcc ccctggatgg gttcgacaat caccgcacag gtggcgtcgt caatcagcgc
    10081 actggcagag ttaatatcgt tatatgcagc atgacgaata tccggcggca gtggcgcaaa
    10141 atcctgtgaa taggctggct gcccacccgc actgacagta aacagcgtgc gaccatgaaa
    10201 cgcatttttg aacgccacga tgccgctctt atggctgccg tagcggtcgt gagcgaattt
    10261 acgcgccagt tttagcgccg cttcgttggc ttccgcaccg gagttacaaa agaagacgcg
    10321 atcggcaaac gtggcgtcga tcaatttttt cgccagtcgc agtaccggct cgttggtgta
    10381 accgttgccg gtatgccaga acttactcgc ctgttcgttc agcgcctcac gcagttccgg
    10441 atgcgcatgg cccagcgcgt tcaccgcaat gccacccgcg aagtcgatat actctttccc
    10501 ctgctgctcc cacaagcgcg aaccttcgcc acgtaccggt ataaacggtg ccggagcgta
    10561 aacaggtatc atccattcat caaagttttc acgcgtaatt ggctgagaca tagcgacctc
    10621 tacagtaaat aattagttat ttatatgtta ataataagta atgtttgcgc tgtaaatgta
    10681 gattgcaggg ttcgtgccag ccagtgataa aagtgcataa acggcggagg ctaactggaa
    10741 atcaagcagt tataaccaca tcatatgcat ttaaagtgca tataaagtga atacgtttgc
    10801 gatgtgggtg aataaaaaga ataaaaaacg caatgttatg cagaagtaaa atataattct
    10861 ggaattgtga tcattgacga aatttactgg aaattactgc gccattctga cgcaacgcgc
    10921 accaaaagcg ggcatttttt gcgccatcgt tgacatcatt aacaaccatc gatcaaatca
    10981 cttaacaaca ggcggtaagc aaagcgaaat tctgctacca tccacgcact ctttatctga
    11041 ataaatggca gcgactatga aatttgtctc ttttaatatc aacggcctgc gcgccagacc
    11101 tcaccagctt gaagccatcg tcgaaaagca ccaaccggat gtgattggcc tgcaggagac
    11161 aaaagttcat gacgatatgt ttccgctcga agaggtggcg aagctcggct acaacgtgtt
    11221 ttatcacggg cagaaaggcc attatggcgt ggcgctgctg accaaagaga cgccgatttc
    11281 cgtgcgtcgc ggctttcccg gtgacgacga agaggcgcaa cggcggatta ttatggcgga
    11341 aatcccctcg ccgctgggca atatcaccgt gatcaacggt tacttcccgc agggtgaaag
    11401 ccgcgaccat ccgataaaat tcccggcaaa ggcgcagttt tatcagaatc tgcaaaacta
    11461 cctggaaacc gaactcaagc gtgaaaatcc ggtgctgatt atgggcgata tgaatatcag
    11521 tcctggcgac ctggatattg gcattggcga agagaaccgt aagcgctggc tgcgtaccgg
    11581 aaaatgttct ttccttccgg aggaacgcga atggatggag aggctgatga gctgggggtt
    11641 ggtcgatacc ttccgccatg cgaatccgca aacagcagat cgtttctcat ggtttgatta
    11701 ccgctcaaaa ggttttgacg ataatcgcgg tctgcgcatc gacctgctgc tcgccagcca
    11761 accgctggca gaatgttgcg tagaaaccgg catcgactat gaaattcgca gcatggaaaa
    11821 accgtccgat cacgcccccg tctgggcgac cttccgccgc taatttagct gatctcctgg
    11881 ctcaaactgg gtcaggagaa ttaaccttga gaaaaatcaa caaactgtca gtaatgattt
    11941 gttgcccgcc gtcctttgtt ataccgtcac tgcgttttta gttgtctgac cacttctcta
    12001 ttatcaagtt tgatatagga aactccacga tgaacgctga gcgtaaattt ctttttgcct
    12061 gtcttatttt tgcgctggtc atttacgcta tccacgcttt cggtttattc gatctgctca
    12121 ccgatttacc ccacttacag acactcattc gccagagcgg attgttcggc tatagcctct
    12181 atattctgtt attcatcatt gccaccctct ttctgttacc aggaagcata ctggtgatcg
    12241 cgggaggaat agtttttggc ccgctcttag ggacactact atcattaatt gccgccacgc
    12301 tggcctcctc gtgctcattc cttctggcgc gctggctggg acgtgattta ctgctgaaat
    12361 acgttggcca tagccatacc tttcaggcca ttgaaaaagg cattgcgcgt aacggtattg
    12421 attttcttat tctgacccgc ttaatcccgt tgtttcctta caatattcaa aattacgctt
    12481 acggattaac cacaatcgcc ttctggcctt atacccttat ttcggcactc acgaccctac
    12541 ccggtattgt tatttatacc gtgatggcaa gcgatctcgc caatgaaggc attacgctgc
    12601 gctttatttt acaactctgt ctggcgggcc tggcactgtt tattctcgtc cagctcgcaa
    12661 aactctacgc ccgacacaaa catgtggatc tgtctgcttc tcgccgcagc ccactttctc
    12721 acccaaaaaa tgaaggatag aacgatgttg caacattatt cagtgtcatg gaaaaaagga
    12781 ctggctgcac tctgcttact ggctgttgct gggcttagcg gctgcgatca aaaagagaat
    12841 gcggctgcaa aagtggaata cgacggactt tcgaacagcc aaccgctgcg tgtcgatgcc
    12901 aataaccata cggtaaccat gctggtgcaa attaatggtc gtttcctcac cgacgacact
    12961 cgtcacggta ttgtgtttaa agatggctcc aacggacata aatcgctgtt tatgggttat
    13021 gcgaccccga aagcatttta tgaagccctg aaagaggcag gtggtacgcc gggcgaaaac
    13081 atgacaatgg ataataaaga aacgactcat gtcacaggca gcaaactgga tatttcggtc
    13141 aactggcaag gggcggcaaa agcgtattcc ttcgatgaag tgattgttga cagtaatggc
    13201 aagaaactgg acatgcgctt tggcggtaat ttaacggcag cagaagagaa gaaaacgggt
    13261 tgcctggtgt gtctggatag ctgcccggtc ggcatcgtca gcaatgcaac atacacttat
    13321 ggtgcggtgg aaaaacgcgg cgaagtgaaa ttcaaaggca atgcctcagt tctcccggcg
    13381 gataacacgc tggcaacggt tacctttaaa atcaccgaat aaagcaggat aaaggatgat
    13441 gaagatgcaa tcgcgtaaaa tctggtacta ccgtataacc ctcatcatcc tgttgttcgc
    13501 tgtgctgctg gcatgggcgc tgcttcccgg cgtccatgag tttatcaatc gcagcgttgc
    13561 ggcgtttgcc gccgtggacc aacagggtat agaacgcttt attcagtctt acggcgcact
    13621 agcggcagtt gtctcgttct tgttgatgat tttacaggcc attgctgcac cgctacctgc
    13681 gtttttgatc acctttgcca atgcgtcgct gtttggcgcg ttctggggcg gcttactgtc
    13741 gtggaccagt tcgatggccg gcgcggcgct gtgctttttt atcgccagag tgatgggccg
    13801 cgaagtggtg gaaaaattaa ccggcaaaac cgtacttgac agtatggacg gctttttcac
    13861 tcgctacggc aaacacacca tcctggtatg tcggttattg ccttttgtcc ctttcgatcc
    13921 aatcagctat gctgccggtt tgacttcaat acgttttcgc tcgtttttta tcgccaccgg
    13981 gcttggtcaa ttaccggcga ctattgttta ttcctgggcg ggcagcatgt taacaggcgg
    14041 tactttctgg tttgtcaccg gactgtttat tctgtttgcc ctgaccgtgg tgatttttat
    14101 ggcgaagaaa atatggcttg agcgccagaa gaggaatgcc tgatgggttt accgccgctt
    14161 agcaaaattc ctttcatttt acgtccgcag gcgtggctgc atcgtcgcca ttacggcgag
    14221 gtgctaagcc cgattcgctg gtgggggcgg atcccgttta tcttttatct ggtgtcgatg
    14281 tttgtcggct ggctggagcg caaacgctca ccgctcgatc cggtagtacg atcgcttgtc
    14341 agcgcgcgca ttgcgcaaat gtgcctgtgt gagttttgcg tggatatcac cagtatgaaa
    14401 gtcgccgagc gtaccggcag caccgataaa ctgctggcag tggctgactg gcggcaaagc
    14461 ccgctcttta gcgatgaaga acggctggcg ctggagtacg ccgaagccgc aagcgtaacg
    14521 ccgccaacgg tcgatgatgc tctgcgcgcc cgactggcta cgcattttga cgctcaggcg
    14581 ctcaccgaac tgacggcatt gataggcctg caaaatctgt cagcccgttt taattctgcc
    14641 atggacattc ccgctcaggg gctgtgccgt attcctgaaa aacgttctta aggagagaag
    14701 atgcgccatt gtgggtggtt gctgggattg ttatcgctgt tttctttggc aacacatgcc
    14761 agtgactggc aggaaataaa aaatgaggcc aaaggacaaa ccgtctggtt taacgcctgg
    14821 ggcggcgata ccgcaattaa ccgctatctc gactgggtta gcggcgagat gaaaacccat
    14881 tacgctataa acctgaagat tgttcgtctc gcggatgccg cagacgcggt gaaacgcatt
    14941 cagaccgaag ttgctgccgg acgtaaaacg ggcggttcgg tggatctgct ctgggtgaac
    15001 ggcgaaaact tccgcacctt aaaagaggcc aatttattac aaaccggctg ggcggagact
    15061 ctgcccaact ggcgctatgt cgacacacag cttccggtgc gggaagattt ttcagtgcct
    15121 acagaagggg ctgaatcgcc ctggggcggc gcacaactga cgtttatcgc ccgccgcgat
    15181 gttacgctac agccaccaca aacgccgcaa gccttactgg agtttgctaa agccaatccc
    15241 ggcacggtta cctatccgcg cccaccggac tttaccggca cggcgtttct tgaacagttg
    15301 ctgattatgc taacgccaga tcccacagca ttaaaagaag cgccggacga tgccactttc
    15361 gcccgcgtca ctgcgccctt gtggcaatat cttgatgcac tgcatccgta tttgtggcgc
    15421 gaaggaaagg atttcccgcc ttcgcccgcg cggatggatg ctctgctgaa agctaacaca
    15481 ttgcgcctgt cgctgacctt taaccccgcg catgcgcagc aaaaaatcgc cagcggcgat
    15541 ttgcctgcaa gcagttacag ttttggtttt cgcgagggga tgattggcaa cgtccatttc
    15601 gtcaccattc ctgccaacgc gaatgccagt gctgcggcga aggtagttgc caatttcctg
    15661 ctctcacccg atgcgcaact gcgtaaagca gatcctgctg tctggggcga tccttctgtt
    15721 ctcgacccgc aaaaactgcc tgacgggcag cgcgaaacat tgcaatcgag aatgccgcag
    15781 gatctgccgc cggtactggc tgaaccgcac gcaggttggg taaatgcgct ggaacaagaa
    15841 tggctacacc gttacggtac gcattaatct ttttgctgtg ggcgatgata gcggtgattt
    15901 atgcaccgct gatcccggca gctctcacgc tgatatcgcc tgctttgtcg ttgacgcact
    15961 ggcaggcgtt atttgccgat ccacagttac cgcaggcatt actggcgacg ctggtgtcga
    16021 caaccattgc ggcggtcggg gcgttgttga ttgccctgct ggtgattgtg gcgttgtggc
    16081 cagggccgaa atggcagcgt atgtgcgccc gtctgccgtg gctgctcgcc attccccatg
    16141 tggcttttgc caccagcgcc cttctgctct ttgctgacgg agggctgctt tatgactatt
    16201 tcccgtattt cactccgcca atggacaaat tgggtattgg gctgggcctc accctggcgg
    16261 tgaaagaaag cgcatttctg ctgtggatct tagcggcagt attgagcgaa aaacggctgt
    16321 tgcagcaact cattgtgctg gattcgctgg gctacagccg ctggcaatgc ctgaactggc
    16381 tgctgttgcc ctccgtcgcg tctgcgttag caatggcgat gctggcgatt gtcgcctggt
    16441 cgctgtcggt cgtggatgtg gcgattattc ttgggccagg taatcccccg acgctggcgg
    16501 taattagctg gcagtggtta actcagggcg acgccgatca acaaacgaaa ggcgcactcg
    16561 ccagcctgct gctgatgctg ttactcgccg cctacgtttt gctgggctat ctgctatggc
    16621 gtggctggcg gcgtactatt ccccgcgtag atggcgttcg taagcctgcc acgcctttat
    16681 tgccaagcaa tacgctggcg agttttttac ccttaaccgg tgtgctgtgt gtggttctgc
    16741 tggcgatcct cgcggatcag tcgacgatca atagtcaagc gctcatcaac agcctgacaa
    16801 tggggctggt ggcggcattc atcgctttgc tcttgttact gctgtggctg gaatgggggc
    16861 cacagcgtcg ccagttgtgg ctatggttgc ccattttatt acccgctctg ccgctggtgg
    16921 cgggccagta cacgctggcg ctatggctga acctggatgg aagctggacg gcggtggtct
    16981 gggggcatct gctgtgggta atgccgtgga tgctgtttat cctgcaaccc gcctggcagc
    17041 gcattgattc acggttaatt ttgattgcgc aaacactggg ctggtcgcgg gccaaaatct
    17101 tcttttacgt aaaatgccca ctcatgttgc gccctgcgct gattgccttc gcggtgggat
    17161 tttcagtcag tattgcgcag tatatgccga cgctgtggct gggcgcgggg cgttttccga
    17221 cgctcaccac tgaagcggtg gcattaagca gcggcggcag caacggtatt ctcgccgccc
    17281 aggctttatg gcaactgctg ttaccgctta ttatttttgc cctgaccgcg ttagtcgcaa
    17341 aatgggtagg ttatgtcaga caaggactcc gctaatgctc tgcgtgaaaa atgtttcgct
    17401 acgtttacca gaaagccgct tgctgacaaa cgttaacttt acagtagata aaggtgacat
    17461 tgtcacgtta atgggaccat ccggctgcgg gaaatccact ctgttttcgt ggatgattgg
    17521 tgcgctggcc ggacagtttt cttgtacagg ggagctatgg cttaatgagc aacggattga
    17581 catgctaccc accgcacagc gtcagattgg cattcttttc caggatgcac tgttatttga
    17641 ccagttcagc gtcgggcaaa atttactgct ggcgctaccg gcgacactta aggggaatgc
    17701 ccgacgtaat gccgtgaatg atgcacttga gcgttcaggc cttgacggag cgttccatca
    17761 ggatcctgcc actttgtctg gcggtcagcg agcgcgcgtt gctctgcttc gcgcccttct
    17821 cgctcagcca aaagcgttgc tactggatga gccattcagc cgtcttgatg tggccctgcg
    17881 cgataatttt cgccattggg tgttcagcga aattcgcgcc atggcgatcc ctgttgtgca
    17941 ggtgacgcac gatcttcagg atgttcctcc tgatagcccc gttctggata tggcgcagtg
    18001 gtcagaaaat tacaacaaac tgcgataacg caaagttttt cccgatgcgt cagttcagaa
    18061 tggcgctctc aaaactacaa tgtcgggatt ttcgatgaaa cgtgtttctc aaatgaccgc
    18121 gctggcaatg gctttagggc tggcttgcgc ttcttcgtgg gccgctgaac tggcgaagcc
    18181 tcttacactt gaccagcttc aacaacaaaa tggcaaagcg atagatactc gccccagcgc
    18241 gttttataac ggctggccac aaaccttaaa tggcccttcc ggtcatgaac ctgccgcctt
    18301 aaacctctct gctagctggc tcgacaaaat gagcaccgaa cagctcaacg agtggatcaa
    18361 gcaacataac ctgaaagccg atgccccggt ggcactgtac ggtaatgaca aagatgtcga
    18421 cgccgtcaaa acgcgactgc aaaaagcagg ttttacgcat atctccatcc tgagtgacgc
    18481 gctaagcgaa ccttcccgtc tgcaaaaact gccgcacttt gaacagctgg tatatccgca
    18541 gtggctgcat gacctgcaac aaggtaaaga ggttacggcg aaacctgccg gtgactggaa
    18601 agtcattgaa gcggcctggg gcgctccaaa attttacctt atcagccata ttcccggcgc
    18661 tgactacatc gacaccaacg aagtggaaag cgaaccgctg tggaacaaag tttctgatga
    18721 acaactgaaa gcgatgctgg caaaacacgg cattcgccat gacaccacgg tcattctgta
    18781 cgggcgtgac gtatacgctg cagcgcgtgt ggcgcaaatt atgctttatg ccggcgtgaa
    18841 agatgtgcgc ctgctggacg gaggctggca aacctggtcc gacgcgggtc tgcccgttga
    18901 gcgcggtacg ccaccgaaag tgaaagcgga accggatttc ggcgtgaaga tcccggcaca
    18961 accgcaactg atgcttgata tggaacaagc gcgtggactg ctgcatcgcc aggatgcatc
    19021 gctggtgagc attcgttcgt ggccagaatt tatcggtacg accagcggtt acagctatat
    19081 taaaccaaaa ggtgaaatag ccggagcacg ttggggacac gcaggtagcg actcgacgca
    19141 tatggaagat ttccataacc cggatggcac catgcgcagc gccgatgata taaccgctat
    19201 gtggaaagca tggaatatca aaccagatca gcaagtttca ttctactgcg gcactggctg
    19261 gcgcgcgtca gaaaccttta tgtacgcacg cgcaatgggc tggaataacg tctccgttta
    19321 cgacggtggc tggtacgaat ggagcagcga tccaaaaaat ccggtagcaa ccggtgaacg
    19381 cggcccggac agcagtaaat aacattgcaa ttactgacgc tggagcgact tcagcgtcag
    19441 atacccactc cacacccgcg taaaggtcgt catccagcac aacgcaccaa atacccacgc
    19501 aaaccacgga aaacaggccg gaaagaggca actcagaaca aacagcaaaa tcgtctcgct
    19561 gccttcggtt aagccgccca gataataaaa cgatttatgc gcataaccag gattatcaat
    19621 ctgatgtttc gccgccagcg cagcaaaagc gagaaaactg ctgcccgtgc cgataaacgc
    19681 aaacagcagc cagccgcctg ccagggcgtt ttgctccggt gcggcgagga taaacccaaa
    19741 aggcaccagc gcgtaaaaaa gaaaatcgag agaaatatcg agaaagccgc ccgcgtcggt
    19801 aagccctctg cgccgcgcca gcgcaccgtc cagaccatca agcagcctgt tcaacaaaat
    19861 ggcgactagt gccgccagat accagcccag cgccagaaaa ggcagtgcca gcacgccgat
    19921 ggcaaatccc actaatgtta aaccatctgg cgtaatgccc ggtttatcaa gaacccgcac
    19981 gcactgatgc aacaacggtt taatccgggg atgaagatgg cggtctagca caggcactcc
    20041 ttaaatataa agcctttctg attgagcaac agtgcggata ttatggcatt tttcgcttat
    20101 ctgcccgtgt gtaatttatg aaaatgattg aagttgttgc cgccatcatc gaacgtgatg
    20161 gcaaaatttt actcgcgcaa cgccctgccc agagcgatca ggcgggatta tgggagtttg
    20221 ccggtggtaa aatcgagccg gatgaaagcc agcggcaggc gctggtgcgt gagttaaacg
    20281 aagaactggg catcgaagca actgttggtg actatgttgc cagccatcag cgagaagttt
    20341 cggggcggat tatccatctt catgcctggc acgtacccga cttccacggg acgttacagg
    20401 cacatgaaca tcaggcgctg gtctggtgct cacctgaaga ggcgctgcga tatccgctgg
    20461 cccctgctga cattccatta ttagaggcgt ttatggcttc acgcgccgcc agaccagcgg
    20521 attagtgctt agggttttgt catcccgttg gcattgcagc agtattcctt cggctttaat
    20581 tactgcccct tcagaataat tttgatcctg ataaacgcag cactgagtac agggctgcgc
    20641 tgactgcccg cctgaactga acacttctgg cggtacgttt acctccacgt ccggatgata
    20701 atgcgggtta gccagtgtaa ttaatggaaa tgctaatact acggcgatca atgctcgact
    20761 cacagggaac tccttaacgt tattgtctct tctacagata acggtagccg ggcggcaaaa
    20821 ctttagcgtc tgagttatcg catttggtta tgagattact ctcgttatta atttgctttc
    20881 ctgggtcatt tttttcttgc ttaccgtcac attcttgatg gtatagtcga aaactgcaaa
    20941 agc
//
LOCUS       DAHHTG010000077        20269 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.077, whole genome shotgun sequence.
ACCESSION   DAHHTG010000077 DAHHTG010000000
VERSION     DAHHTG010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20269)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20269)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20269
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.077"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            85..1554
                     /gene="gspA"
                     /locus_tag="NBI74_002880"
     CDS             85..1554
                     /gene="gspA"
                     /locus_tag="NBI74_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspA"
                     /protein_id="HCD1933175.1"
                     /translation="MSTRREVILSWLREKHQTWRLCYLLGEAGSGKTRLAQQLQKDKH
                     RRVITLSLVVSWQGKAAWIVTDDNAAEQGCRDSAWTRDEMAGQLLHALHRTDSRCPLI
                     IIENAHLNHRRILDDLQRAISLIPDGQFLLIGRPDRKVERDFKKQGIELVSTGRLTEH
                     ELKASILEGQNIDQPDLLLTARVLKRIALLCRGDRRKLALAGETISLLQQAEQTCVFT
                     AKQWRMIYRVLGDNRPRKMQLAVVMSGTIIALTCGWLLLPSFTAPLPVPAWLIPVTPV
                     VKQDMTKDIAHVVMRDSEALSVLYGVWGYEVPADSAWCDQAVRAGLVCKSGNASLQTL
                     VDQNLPWIASLKVGDKKLPVVVVRVGDATVDVLVGQQTWTLTHKWFELVWTGDYLLLW
                     KMSPEGESTITRDSSEEEILWLETMLNRALHISTESSAEWRPLLVEKIKQFQKSHHLK
                     TDGVVGFSTLVHLWQVAGESAYLYRDEANISPETTVKGK"
     gene            1556..1975
                     /gene="gspB"
                     /locus_tag="NBI74_002881"
     CDS             1556..1975
                     /gene="gspB"
                     /locus_tag="NBI74_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative general secretion pathway protein GspB"
                     /protein_id="HCD1933176.1"
                     /translation="MFEFYIAAREQKETGHPGIFSRQKHSTIIYVICLLLICLWFAGM
                     VLVGGYARQLWALWIVKAEVTVDAETPAFKQSTQHYFFKKQPLPVVESVEEEDDPGVA
                     VENAPSSSEDEDNTVEESDEKAGLRERVKNALNELER"
     gene            2213..2524
                     /gene="rpsJ"
                     /locus_tag="NBI74_002882"
     CDS             2213..2524
                     /gene="rpsJ"
                     /locus_tag="NBI74_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014268624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HCD1933177.1"
                     /translation="MQNQRIRIRLKAFDHRLIDQATAEIVETAKRTGAQVRGPIPLPT
                     RKERFTVLISPHVNKDARDQYEIRTHLRLVDIVEPTEKTVDALMRLDLAAGVDVQISL
                     G"
     gene            2557..3186
                     /gene="rplC"
                     /locus_tag="NBI74_002883"
     CDS             2557..3186
                     /gene="rplC"
                     /locus_tag="NBI74_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000579825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HCD1933178.1"
                     /translation="MIGLVGKKVGMTRIFTEDGVSIPVTVIEVEANRVTQVKDLANDG
                     YRAIQVTTGAKKANRVTKPEAGHFAKAGVEAGRGLWEFRLAEGEEFTVGQSISVELFA
                     DVKKVDVTGTSKGKGFAGTVKRWNFRTQDATHGNSLSHRVPGSIGQNQTPGKVFKGKK
                     MAGQMGNERVTVQSLDVVRVDAERNLLLVKGAVPGATGSDLIVKPAVKA"
     gene            3197..3802
                     /gene="rplD"
                     /locus_tag="NBI74_002884"
     CDS             3197..3802
                     /gene="rplD"
                     /locus_tag="NBI74_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010304939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HCD1933179.1"
                     /translation="MELVLKDAQSALTVSETTFGRDFNEALVHQVVVAYAAGARQGTR
                     AQKTRAEVTGSGKKPWRQKGTDRARSGSIKSPIWRSGGVTFAARPQDHSQKVNKKMYR
                     GALKSILSELVRQDRLIVVEKFSVEAPKTKLLAQKLKDMALEDVLIITGELDENLFLA
                     ARNLHKVDVRDATGIDPVSLIAFDKVVMTADAVKQVEEMLA"
     gene            3799..4101
                     /gene="rplW"
                     /locus_tag="NBI74_002885"
     CDS             3799..4101
                     /gene="rplW"
                     /locus_tag="NBI74_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007891652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HCD1933180.1"
                     /translation="MIREERLLKVLRAPHVSEKASTAMEKSNTIVLKVAKDATKAEIK
                     AAVQKLFEVEVEVVNTLVVKGKVKRHGQRIGRRSDWKKAYVTLKEGQNLDFVGGAE"
     gene            4119..4940
                     /gene="rplB"
                     /locus_tag="NBI74_002886"
     CDS             4119..4940
                     /gene="rplB"
                     /locus_tag="NBI74_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HCD1933181.1"
                     /translation="MAVVKCKPTSPGRRHVVKVVNPELHKGKPFAPLLEKNSKSGGRN
                     NNGRITTRHIGGGHKQAYRIVDFKRNKDGIPAVVERLEYDPNRSANIALVLYKDGERR
                     YILAPKGLKAGDQIQSGVDAAIKPGNTLPMRNIPVGSTVHNVEMKPGKGGQLARSAGT
                     YVQIVARDGAYVTLRLRSGEMRKVEADCRATLGEVGNAEHMLRVLGKAGAARWRGVRP
                     TVRGTAMNPVDHPHGGGEGRNFGKHPVTPWGVQTKGKKTRSNKRTDKFIVRRRSK"
     gene            4957..5235
                     /gene="rpsS"
                     /locus_tag="NBI74_002887"
     CDS             4957..5235
                     /gene="rpsS"
                     /locus_tag="NBI74_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HCD1933182.1"
                     /translation="MPRSLKKGPFIDLHLLKKVEKAVESGDKKPLRTWSRRSTIFPNM
                     IGLTIAVHNGRQHVPVFVTDEMVGHKLGEFAPTRTYRGHAADKKAKKK"
     gene            5250..5582
                     /gene="rplV"
                     /locus_tag="NBI74_002888"
     CDS             5250..5582
                     /gene="rplV"
                     /locus_tag="NBI74_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HCD1933183.1"
                     /translation="METIAKHRHARSSAQKVRLVADLIRGKKVSQALDILTYTNKKAA
                     VLVKKVLESAIANAEHNDGADIDDLKVTKIFVDEGPSMKRIMPRAKGRADRILKRTSH
                     ITVVVSDR"
     gene            5600..6301
                     /gene="rpsC"
                     /locus_tag="NBI74_002889"
     CDS             5600..6301
                     /gene="rpsC"
                     /locus_tag="NBI74_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HCD1933184.1"
                     /translation="MGQKVHPNGIRLGIVKPWNSTWFANTKEFADNLDSDFKVRQYLT
                     KELAKASVSRIVIERPAKSIRVTIHTARPGIVIGKKGEDVEKLRKVVADIAGVPAQIN
                     IAEVRKPELDAKLVADSITSQLERRVMFRRAMKRAVQNAMRLGAKGIKVEVSGRLGGA
                     EIARTEWYREGRVPLHTLRADIDYNTSEAHTTYGVIGVKVWIFKGEILGGMAAVEQPE
                     KPAAQPKKQQRKGRK"
     gene            6314..6724
                     /gene="rplP"
                     /locus_tag="NBI74_002890"
     CDS             6314..6724
                     /gene="rplP"
                     /locus_tag="NBI74_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017805229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HCD1933185.1"
                     /translation="MLQPKRTKFRKMHKGRNRGLAQGTDVSFGSFGLKAVGRGRLTAR
                     QIEAARRAMTRAVKRQGKIWIRVFPDKPITEKPLAVRMGKGKGNVEYWVALIQPGKVL
                     YEMDGVPEELAREAFKLAAAKLPIKTTFVTKTVM"
     gene            6724..6915
                     /gene="rpmC"
                     /locus_tag="NBI74_002891"
     CDS             6724..6915
                     /gene="rpmC"
                     /locus_tag="NBI74_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000644743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HCD1933186.1"
                     /translation="MKAKELREKSVEELNTELLNLLREQFNLRMQAASGQLQQSHLLK
                     QVRRDVARVKTLLNEKAGA"
     gene            6915..7169
                     /gene="rpsQ"
                     /locus_tag="NBI74_002892"
     CDS             6915..7169
                     /gene="rpsQ"
                     /locus_tag="NBI74_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006817275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HCD1933187.1"
                     /translation="MTDKIRTLQGRVVSDKMEKSIVVAIERFVKHPIYGKFIKRTTKL
                     HVHDENNECGIGDVVEIRECRPLSKTKSWTLVRVVEKAVL"
     gene            7334..7705
                     /gene="rplN"
                     /locus_tag="NBI74_002893"
     CDS             7334..7705
                     /gene="rplN"
                     /locus_tag="NBI74_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015817037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HCD1933188.1"
                     /translation="MIQEQTMLNVADNSGARRVMCIKVLGGSHRRYAGVGDIIKITIK
                     EAIPRGKVKKGDVLKAVVVRTKKGVRRPDGSVIRFDGNACVLLNNNSEQPIGTRIFGP
                     VTRELRSEKFMKIISLAPEVL"
     gene            7716..8030
                     /gene="rplX"
                     /locus_tag="NBI74_002894"
     CDS             7716..8030
                     /gene="rplX"
                     /locus_tag="NBI74_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000729185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HCD1933189.1"
                     /translation="MAAKIRRDDEVIVLTGKDKGKRGKVKNVLSSGKVIVEGINLVKK
                     HQKPVPALNQPGGIVEKEAAIQVSNVAIFNATTGKADRVGFRFEDGKKVRFFKSNSET
                     IK"
     gene            8045..8584
                     /gene="rplE"
                     /locus_tag="NBI74_002895"
     CDS             8045..8584
                     /gene="rplE"
                     /locus_tag="NBI74_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HCD1933190.1"
                     /translation="MAKLHDYYKDEVVKKLMTEFNYNSVMQVPRVEKITLNMGVGEAI
                     ADKKLLDNAAADLAAISGQKPLITKARKSVAGFKIRQGYPIGCKVTLRGERMWEFFER
                     LITIAVPRIRDFRGLSAKSFDGRGNYSMGVREQIIFPEIDYDKVDRVRGLDITITTTA
                     KSDEEGRALLAAFDFPFRK"
     gene            8599..8904
                     /gene="rpsN"
                     /locus_tag="NBI74_002896"
     CDS             8599..8904
                     /gene="rpsN"
                     /locus_tag="NBI74_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HCD1933191.1"
                     /translation="MAKQSMKAREVKRVALADKYFAKRAELKAIISDVNASDEDRWNA
                     VLKLQTLPRDSSPSRQRNRCRQTGRPHGFLRKFGLSRIKVREAAMRGEIPGLKKASW"
     gene            8938..9330
                     /gene="rpsH"
                     /locus_tag="NBI74_002897"
     CDS             8938..9330
                     /gene="rpsH"
                     /locus_tag="NBI74_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HCD1933192.1"
                     /translation="MSMQDPIADMLTRIRNGQAANKAAVTMPSSKLKVAIANVLKEEG
                     FIEDFKVEGDTKPELELTLKYFQGKAVVESIQRVSRPGLRIYKRKDELPKVMAGLGIA
                     VVSTSKGVMTDRAARQAGLGGEIICYVA"
     gene            9343..9876
                     /gene="rplF"
                     /locus_tag="NBI74_002898"
     CDS             9343..9876
                     /gene="rplF"
                     /locus_tag="NBI74_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003031129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HCD1933193.1"
                     /translation="MSRVAKAPVVVPAGVDVKINGQVITIKGKNGELTRTLNDAVEVK
                     HADNTLTFGPRDGYADGWAQAGTARALLNSMVIGVTEGFTKKLQLVGVGYRAAVKGNV
                     INLSLGFSHPVDHQLPAGITAECPTQTEIVLKGADKQVIGQVAADLRAYRRPEPYKGK
                     GVRYADEVVRTKEAKKK"
     gene            9886..10239
                     /gene="rplR"
                     /locus_tag="NBI74_002899"
     CDS             9886..10239
                     /gene="rplR"
                     /locus_tag="NBI74_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HCD1933194.1"
                     /translation="MDKKSARIRRATRARRKLQELGATRLVVHRTPRHIYAQVIAPNG
                     SEVLVAASTVEKAIAEQLKYTGNKDAAAAVGKAVAERALEKGIKDVSFDRSGFQYHGR
                     VQALADAAREAGLQF"
     gene            10254..10757
                     /gene="rpsE"
                     /locus_tag="NBI74_002900"
     CDS             10254..10757
                     /gene="rpsE"
                     /locus_tag="NBI74_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004106339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HCD1933195.1"
                     /translation="MAHIEKQAGELQEKLIAVNRVSKTVKGGRIFSFTALTVVGDGNG
                     RVGFGYGKAREVPAAIQKAMEKARRNMINVALNNGTLQHPVKGVHTGSRVFMQPASEG
                     TGIIAGGAMRAVLEVAGVHNVLAKAYGSTNPINVVRATIDGLENMNSPEMVAAKRGKS
                     VEEILGK"
     gene            10761..10940
                     /gene="rpmD"
                     /locus_tag="NBI74_002901"
     CDS             10761..10940
                     /gene="rpmD"
                     /locus_tag="NBI74_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012147215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HCD1933196.1"
                     /translation="MAKTIKITQTRSAIGRLPKHKATLLGLGLRRIGHTVEREDTPAI
                     RGMINAVSFMVKVEE"
     gene            10944..11378
                     /gene="rplO"
                     /locus_tag="NBI74_002902"
     CDS             10944..11378
                     /gene="rplO"
                     /locus_tag="NBI74_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011095501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HCD1933197.1"
                     /translation="MRLNTLSPAEGSKKAGKRLGRGIGSGLGKTGGRGHKGQKSRSGG
                     GVRRGFEGGQMPLYRRLPKFGFTSRKAAITAEVRLSDLAKVEGGVVDLNTLKAANIIG
                     IQIEFAKVILAGEVTTPVTVRGLRVTKGARAAIEAAGGKIEE"
     gene            11386..12717
                     /gene="secY"
                     /locus_tag="NBI74_002903"
     CDS             11386..12717
                     /gene="secY"
                     /locus_tag="NBI74_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004868351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HCD1933198.1"
                     /translation="MAKQPGLDFQSAKGGLGELKRRLLFVIGALIVFRIGSFIPIPGI
                     DAAVLAKLLEQQRGTIIEMFNMFSGGALSRASIFALGIMPYISASIIIQLLTVVHPTL
                     AEIKKEGESGRRKISQYTRYGTLVLAIFQSIGIATGLPNMPGMQGLVINPGFAFYFTA
                     VVSLVTGTMFLMWLGEQITERGIGNGISIIIFAGIVAGLPPAIAHTIEQARQGDLHFL
                     VLLLVAVLVFAVTFFVVFVERGQRRIVVNYAKRQQGRRVYAAQSTHLPLKVNMAGVIP
                     AIFASSIILFPATIASWFGGGTGWNWLTTISLYLQPGQPLYVLLYASAIIFFCFFYTA
                     LVFNPRETADNLKKSGAFVPGIRPGEQTAKYIDKVMTRLTLVGALYITFICLIPEFMR
                     DAMKVPFYFGGTSLLIVVVVIMDFMAQVQTLMMSSQYESALKKANLKGYGR"
     gene            12749..12865
                     /gene="rpmJ"
                     /locus_tag="NBI74_002904"
     CDS             12749..12865
                     /gene="rpmJ"
                     /locus_tag="NBI74_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020322760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HCD1933199.1"
                     /translation="MKVRASVKKLCRNCKIVKRDGVIRVICSAEPKHKQRQG"
     gene            13012..13368
                     /gene="rpsM"
                     /locus_tag="NBI74_002905"
     CDS             13012..13368
                     /gene="rpsM"
                     /locus_tag="NBI74_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019935869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HCD1933200.1"
                     /translation="MARIAGINIPDHKHAVIALTSIYGVGKTRSKAILAAAGIAEDVK
                     ISELSEGQIDTLRDEVAKFVVEGDLRREISMSIKRLMDLGCYRGLRHRRGLPVRGQRT
                     KTNARTRKGPRKPIKK"
     gene            13385..13774
                     /gene="rpsK"
                     /locus_tag="NBI74_002906"
     CDS             13385..13774
                     /gene="rpsK"
                     /locus_tag="NBI74_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HCD1933201.1"
                     /translation="MAKAPIRARKRVRKQVSDGVAHIHASFNNTIVTITDRQGNALGW
                     ATAGGSGFRGSRKSTPFAAQVAAERCADVVKEYGIKNLEVMVKGPGPGRESTIRALNA
                     AGFRITNITDVTPIPHNGCRPPKKRRV"
     gene            13808..14428
                     /gene="rpsD"
                     /locus_tag="NBI74_002907"
     CDS             13808..14428
                     /gene="rpsD"
                     /locus_tag="NBI74_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004410410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HCD1933202.1"
                     /translation="MARYLGPKLKLSRREGTDLFLKSGVRAIDTKCKIEQAPGQHGAR
                     KPRLSDYGVQLREKQKVRRIYGVLERQFRNYYKEAARLKGNTGENLLALLEGRLDNVV
                     YRMGFGATRAEARQLVSHKAIMVNGRVVNIASYQVSPNDVVSIREKAKKQSRVKAALE
                     LAEQREKPTWLEVDAGKMEGTFKRKPERSDLSADINEHLIVELYSK"
     gene            14454..15443
                     /gene="rpoA"
                     /locus_tag="NBI74_002908"
     CDS             14454..15443
                     /gene="rpoA"
                     /locus_tag="NBI74_002908"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HCD1933203.1"
                     /translation="MQGSVTEFLKPRLVDIEQVSSTHAKVTLEPLERGFGHTLGNALR
                     RILLSSMPGCAVTEVEIDGVLHEYSTKEGVQEDILEILLNLKGLAVRVQGKDEVILTL
                     NKSGIGPVTAADITHDGDVEIVKPQHVICHLTDENASISMRIKVQRGRGYVPASTRIH
                     SEEDERPIGRLLVDACYSPVERIAYNVEAARVEQRTDLDKLVIEMETNGTIDPEEAIR
                     RAATILAEQLEAFVDLRDVRQPEVKEEKPEFDPILLRPVDDLELTVRSANCLKAEAIH
                     YIGDLVQRTEVELLKTPNLGKKSLTEIKDVLASRGLSLGMRLENWPPASIADE"
     gene            15484..15867
                     /gene="rplQ"
                     /locus_tag="NBI74_002909"
     CDS             15484..15867
                     /gene="rplQ"
                     /locus_tag="NBI74_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HCD1933204.1"
                     /translation="MRHRKSGRQLNRNSSHRQAMFRNMAGSLVRHEIIKTTLPKAKEL
                     RRVVEPLITLAKTDSVANRRLAFARTRDNEIVAKLFNELGPRFASRAGGYTRILKCGF
                     RAGDNAPMAYIELVDRSEKAEAAAE"
     gene            15974..16342
                     /locus_tag="NBI74_002910"
     CDS             15974..16342
                     /locus_tag="NBI74_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1992 domain-containing protein"
                     /protein_id="HCD1933205.1"
                     /translation="MWLLDQWAERHIAEAQAKGEFDNLVGSGEPLILDDDSHVPPELR
                     AGYRLLKNAGCLPPELEHRREAIQLLDILKGIRHDDPQYQEVSRRLSLLELKLRQAGL
                     STDFLRGDYADKLLNKINDN"
     gene            16353..16778
                     /gene="zntR"
                     /locus_tag="NBI74_002911"
     CDS             16353..16778
                     /gene="zntR"
                     /locus_tag="NBI74_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(2+)-responsive transcriptional regulator"
                     /protein_id="HCD1933206.1"
                     /translation="MYRIGELAKMAEVTPDTIRYYEKQQMMEHEVRTEGGFRLYTESD
                     LQRLKFIRHARQLGFSLESIRELLSIRIDPEHHTCQESKGIVQERLQEVEARIAELQS
                     MQRSLQRLNDTCCGTAHSSVYCSILEALEQGASGVKSGC"
     gene            16834..17052
                     /gene="arfA"
                     /locus_tag="NBI74_002912"
     CDS             16834..17052
                     /gene="arfA"
                     /locus_tag="NBI74_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome-rescue factor ArfA"
                     /protein_id="HCD1933207.1"
                     /translation="MSRYQHTKGQIKDNAIEALLHDPLFRQRVEKNKKGKGSYMRKGK
                     HGNRGNWEASGKKVNHFFTTGLLLSDAC"
     gene            complement(17049..17462)
                     /gene="mscL"
                     /locus_tag="NBI74_002913"
     CDS             complement(17049..17462)
                     /gene="mscL"
                     /locus_tag="NBI74_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large-conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HCD1933208.1"
                     /translation="MSIIKEFREFAMRGNVVDLAVGVIIGAAFGKIVSSLVADIIMPP
                     LGLLIGGIDFKQFAVTLREAQGDIPAVVMHYGVFIQNVFDFLIVAFAIFMAIKLINKL
                     NRKKEEPAAATPAPTKEEVLLTEIRDLLKEQNNRS"
     gene            complement(17592..18968)
                     /gene="trkA"
                     /locus_tag="NBI74_002914"
     CDS             complement(17592..18968)
                     /gene="trkA"
                     /locus_tag="NBI74_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HCD1933209.1"
                     /translation="MKIIILGAGQVGGTLAENLVGENNDITVVDTNGERLRTLQDKFD
                     LRVVQGHGSHPRVLREAGADDADMLVAVTSSDETNMVACQVAYSLFNTPNRIARIRSP
                     DYVRDADKLFHSDAVPIDHLIAPEQLVIDNIYRLIEYPGALQVVNFAEGKVSLAVVKA
                     YYGGPLIGNALSTMREHMPHIDTRVAAIFRHDRPIRPQGSTIVEAGDEVFFIAASQHI
                     RAVMSELQRLEKPYKRIMLVGGGNIGAGLARRLEKDYSVKLIERNQQRAAELAEKLQN
                     TIVFFGDASDQELLAEEHIDQVDLFIAVTNDDEANIMSAMLAKRMGAKKVMVLIQRRA
                     YVDLVQGSVIDIAISPQQATISALLSHVRKADIVGVSSLRRGVAEAIEAVAHGDESTS
                     RVVGRVIDEIKLPPGTIIGAVVRGNDVMIANDNLRIEQGDHVIMFLTDKKFITDVERL
                     FQPSPFFL"
     gene            complement(18990..>20269)
                     /gene="rsmB"
                     /locus_tag="NBI74_002915"
     CDS             complement(18990..>20269)
                     /gene="rsmB"
                     /locus_tag="NBI74_002915"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HCD1933210.1"
                     /translation="RNLRSMAAQAIEQVVEQGQSLSNILPPLQQKVSDKDKALLQELC
                     FGVLRTLSQLDWLINKLMARPMTGKQRTVHYLIMVGLYQLLYTRIPPHAALAETVEGA
                     VAIKRPQLKGLINGVLRQFQRQQDELLAEFNASDARYLHPSWLLKRLQKAYPEQWQSI
                     VEANNQRPPMWLRVNRTHHSRDSWLALLDEAGMKGFPHADYPDAVQLETPAPVHALPG
                     FEEGWVTVQDASAQGCMTWLAPQNGEHILDLCAAPGGKTTHILEVAPEAQVLAVDIDE
                     QRLSRVYDNLKRLGMKATVKQGDGRNPSQWCGEQQFDRILLDAPCSATGVIRRHPDIK
                     WLRRDRDIPELAQLQSEILDAIWPHLKSGGTLVYATCSVLPEENSLQIKAFLQRTADA
                     ELCETGTPEQPGKQNLPGAEEGDGFFYAKLIKK"
BASE COUNT         5215 a         4762 c         5334 g         4958 t
ORIGIN      
        1 tatattagta atatgtattt aattaataaa tacatacatt atttttatga ttaaggataa
       61 tcaataatta aaacaggaag ttctatgtct acgagaagag aagtcattct ttcctggttg
      121 cgtgagaagc atcagacctg gcgtctgtgc tatttgttgg gtgaggctgg aagtggaaaa
      181 acccggctgg cgcagcaact acaaaaagat aaacatcgcc gtgtgattac tttaagcctc
      241 gttgtttcct ggcaaggtaa ggccgcatgg atcgttaccg acgataatgc ggctgaacag
      301 ggctgccgtg acagcgcctg gacgcgagat gagatggcgg gtcaattact gcatgcactt
      361 catcgaactg atagtcgctg ccctcttatt attattgaaa atgctcacct gaatcaccgc
      421 cgcatacttg atgatctcca acgcgcgata tctcttattc ctgacggtca atttttgctg
      481 atcggcagac ccgatcgaaa agtcgaacgt gattttaaaa aacagggcat tgaacttgtc
      541 tcgacaggtc gtctgacgga gcacgagctt aaggcaagca tccttgaagg tcagaatatt
      601 gaccagcccg atctcctttt aaccgccaga gttctgaaac ggatagcttt attatgtcgg
      661 ggagatcgca gaaagctggc gcttgccggc gaaacaatta gcttattaca gcaagccgag
      721 cagacctgcg ttttcactgc aaaacaatgg cgaatgattt accgcgtatt aggtgataac
      781 cgcccccgga aaatgcagct tgccgtggtg atgagtggaa ccattatcgc acttacctgt
      841 ggttggctac tgctgccctc tttcacagct ccgttacccg ttcctgcgtg gctgataccc
      901 gtaacgccag tagttaaaca agatatgact aaggatattg cccatgtggt gatgcgcgat
      961 agtgaggcgc taagcgtgtt atacggcgta tgggggtacg aggtcccagc cgattcggcc
     1021 tggtgcgatc aggcagtaag agcggggctg gtatgtaaaa gtgggaatgc ttctttacaa
     1081 acattggtag atcagaattt gccctggatc gcatcgctta aagtcgggga caaaaaattg
     1141 cctgttgtgg tcgttcgcgt cggtgatgct accgttgatg tactcgtagg gcagcaaacc
     1201 tggacgctga cacataaatg gtttgagttg gtatggacgg gtgattatct tctgttgtgg
     1261 aaaatgtcgc cggaaggaga gagtaccatt acgcgtgata gcagcgagga agagattctc
     1321 tggctggaaa cgatgttaaa ccgcgcattg catatttcga cagaatcttc ggcagagtgg
     1381 cgtcccttgt tggtagaaaa aataaagcaa tttcaaaaaa gccatcactt gaaaactgac
     1441 ggtgttgttg gcttttcaac gttagtgcat ctctggcagg tggcagggga gagtgcctac
     1501 ttatatcggg atgaagcaaa tatttccccg gaaacaacag tgaaggggaa atgacatgtt
     1561 tgaattctat atcgccgcgc gtgagcagaa agaaacaggt caccctggta tcttctcccg
     1621 acaaaaacac agcaccataa tatatgtcat ttgtttattg ctgatttgcc tgtggtttgc
     1681 aggaatggtg ttggttggtg ggtatgcccg gcagctatgg gcactttgga tagtaaaagc
     1741 agaagtcact gtagatgctg aaacgcccgc ttttaaacaa tcaacacaac actatttttt
     1801 caaaaaacag ccactccccg tcgtcgaatc cgtcgaggag gaagatgacc caggagtcgc
     1861 ggttgagaat gccccctctt cttctgaaga cgaagacaat actgtagaag aaagcgacga
     1921 gaaagccggt ttaagagagc gtgtcaaaaa tgcgctgaac gaacttgaga gataacccaa
     1981 aggctgttta ctttactagc aatacgcttg cgttcggtgg ttaagtatgt ataatgcgcg
     2041 ggcttgtcgt agttgacagc aggttcaatc tgaacctcag ttagccgaat ttggctacct
     2101 aacaatgctc ccaatcgggg agctacgtaa gaacggttac actctcccat caatcgtaat
     2161 gggtctgagg agtaatcatt ttcgtttata aaataattgg agctctggtc tcatgcagaa
     2221 ccaaagaatc cgtatccgcc tgaaagcgtt tgatcatcgt ctgatcgatc aagcaaccgc
     2281 ggaaatcgtc gagactgcta agcgcactgg tgcgcaggtc cgtggtccga tcccgctgcc
     2341 gacacgcaaa gagcgcttca ctgttctgat ctccccgcac gtcaacaaag acgcgcgcga
     2401 tcagtacgaa atccgtactc acttgcgtct ggttgacatc gttgagccaa ccgagaaaac
     2461 cgttgatgct ctgatgcgtc tggatctggc tgccggtgta gacgtgcaga tcagcctggg
     2521 ttaatcaggt cattgagcga ttgagaggtt gaaacaatga ttggtttagt cggtaaaaaa
     2581 gtgggtatga cccgtatctt cacagaagac ggcgtttcta tcccagtaac cgtaatcgaa
     2641 gttgaagcaa accgcgttac tcaggttaaa gacctggcta acgatggcta ccgtgctatt
     2701 caggtgacca ccggtgctaa aaaagctaac cgtgtgacca agcctgaagc tggccacttc
     2761 gctaaagctg gcgtagaagc tggccgtggt ctgtgggaat tccgcctggc tgaaggcgaa
     2821 gagttcactg taggtcagag cattagcgtt gaactgtttg ctgacgttaa aaaagttgac
     2881 gtaactggca cctctaaagg taaaggtttc gcaggtaccg ttaagcgctg gaacttccgt
     2941 acccaggacg ctactcacgg taactccttg tctcaccgcg ttccgggttc tatcggtcag
     3001 aaccagactc cgggcaaagt gttcaaaggc aagaaaatgg caggtcagat gggtaacgaa
     3061 cgtgtaaccg ttcagagcct tgacgtagta cgcgttgacg ctgagcgcaa cctgctgctg
     3121 gttaaaggcg ctgtcccggg tgcaaccggt agcgacctga tcgttaaacc agctgtgaag
     3181 gcgtaaggag atagcaatgg aattagtatt gaaagacgcg cagagcgcgc tgactgtttc
     3241 cgaaactacc ttcggtcgtg atttcaacga agcgctggtt caccaggttg ttgttgctta
     3301 tgcagctggt gctcgtcagg gtactcgtgc tcagaagact cgtgctgaag taactggttc
     3361 cggtaaaaaa ccgtggcgcc agaaaggcac cgaccgtgcg cgttctggtt ctatcaagag
     3421 cccgatctgg cgttctggtg gcgtgacctt tgctgctcgt ccgcaggacc acagtcaaaa
     3481 agttaacaag aagatgtacc gcggcgcgct gaaaagcatc ctgtccgaac tggtacgtca
     3541 ggatcgtctg atcgttgtcg agaagttctc tgtagaagcg ccgaaaacta agctgctggc
     3601 acagaaactg aaagacatgg ctctggaaga tgtgctgatc atcaccggtg agctggacga
     3661 gaacctgttc ctggctgcgc gcaacctgca caaggttgac gtacgcgatg caactggtat
     3721 cgacccggtt agcctgatcg ccttcgacaa agtcgtaatg actgctgatg ctgttaagca
     3781 agttgaggag atgctggcat gattcgtgaa gaacgtctgc tgaaggtgct gcgtgcaccg
     3841 cacgtttctg aaaaagcgtc tactgcgatg gaaaaatcca acaccatcgt actcaaagtt
     3901 gctaaagacg cgaccaaagc agaaatcaaa gctgctgtgc agaaactgtt tgaagtcgaa
     3961 gtcgaagtcg ttaacaccct ggtagttaaa gggaaagtta aacgtcacgg acagcgtatc
     4021 ggtcgtcgta gcgactggaa aaaagcttac gtcaccctga aagaaggcca gaatctggac
     4081 ttcgttggcg gcgctgagta agtcggagga gtaatacaat ggcagttgtt aaatgtaaac
     4141 cgacatctcc gggtcgtcgc cacgtagtta aagtggttaa ccctgagctg cacaagggca
     4201 aaccttttgc tccgttgctg gaaaaaaaca gcaaatccgg tggtcgtaac aacaatggcc
     4261 gtatcaccac tcgtcatatc ggtggtggcc acaagcaggc ttaccgtatt gttgacttca
     4321 aacgcaacaa agacggtatc ccggcagttg ttgaacgtct tgagtacgat ccgaaccgtt
     4381 ccgcgaacat cgcgctggtt ctgtacaaag acggtgaacg ccgttacatc ctggccccta
     4441 aaggcctgaa agctggcgac cagattcagt ctggcgttga tgctgcaatc aaaccaggta
     4501 acaccctgcc gatgcgcaac atcccggttg gttctactgt tcataacgta gaaatgaaac
     4561 caggtaaagg cggtcagctg gcacgttccg ctggtactta cgttcagatc gttgctcgtg
     4621 atggtgctta tgtcaccctg cgtctgcgtt ctggtgaaat gcgtaaagta gaagcagact
     4681 gccgtgcaac tctgggcgaa gttggcaatg ctgagcatat gctgcgcgtt ctgggtaaag
     4741 caggtgctgc acgctggcgt ggtgttcgtc cgaccgttcg cggtaccgcg atgaacccgg
     4801 tagaccaccc acatggtggt ggtgaaggtc gtaactttgg taagcacccg gtaactccgt
     4861 ggggcgttca gaccaaaggt aagaagaccc gcagcaacaa gcgtactgat aaattcatcg
     4921 tacgtcgccg tagcaaataa ttttagagga taagccatgc cacgttctct caagaaaggt
     4981 ccttttattg acctgcactt gctgaagaag gtagagaaag cggtggaaag cggagacaag
     5041 aagcccctgc gcacttggtc ccgtcgttca acgatctttc ctaacatgat cggtttgacc
     5101 atcgctgtcc ataatggtcg tcagcacgtt ccggtatttg taaccgacga aatggtcggt
     5161 cacaaactgg gtgaattcgc accgactcgt acttatcgcg gccacgctgc tgataaaaaa
     5221 gcgaagaaga aataaggtag gaggaagaga tggaaactat cgctaaacat cgccatgctc
     5281 gttcttctgc tcagaaggtt cgccttgttg ctgacctgat tcgcggtaag aaagtgtcgc
     5341 aggctctgga tattttgacc tacaccaaca agaaagcggc tgtactggtc aagaaagttc
     5401 tggaatctgc cattgctaac gctgaacaca acgatggcgc tgacattgac gatctgaaag
     5461 ttacgaaaat tttcgtagac gaaggcccga gcatgaagcg cattatgccg cgtgcaaaag
     5521 gtcgtgcaga tcgcatcctg aagcgcacca gccacatcac tgtggttgtg tccgatcgct
     5581 gagactctgg agactagcaa tgggtcagaa agtacatcct aatggtattc gcctgggtat
     5641 tgtaaaacca tggaactcta cctggtttgc gaacaccaaa gaattcgctg acaacctgga
     5701 cagcgatttt aaagtacgtc agtacctgac taaggaactg gctaaagcgt ccgtatctcg
     5761 tatcgttatc gagcgtccgg ctaagagcat ccgtgtaacc attcacactg ctcgcccggg
     5821 tatcgttatc ggtaaaaaag gtgaagacgt agaaaaactg cgtaaggtcg tagcggacat
     5881 cgctggcgtt cctgcacaga tcaacatcgc cgaagttcgt aagcctgaac tggacgcaaa
     5941 actggttgct gacagcatca cttctcagct ggaacgtcgc gttatgttcc gtcgtgctat
     6001 gaagcgtgct gtacagaacg caatgcgtct gggcgctaaa ggtattaaag ttgaagttag
     6061 cggccgtctg ggcggcgcgg aaatcgcacg taccgaatgg taccgcgaag gtcgcgtacc
     6121 gctgcacact ctgcgtgctg acatcgacta caacacctct gaagcgcaca ccacttacgg
     6181 tgtaatcggc gttaaagtgt ggatcttcaa aggcgagatc ctgggtggta tggctgctgt
     6241 tgaacaaccg gaaaaaccgg ctgctcagcc taaaaagcag cagcgtaaag gccgtaaata
     6301 aggagcgtcg ctgatgttac aaccaaagcg tacaaaattc cgtaaaatgc acaaaggccg
     6361 taaccgcggt ctggcgcagg gtacggatgt tagcttcggc agcttcggtc tgaaagctgt
     6421 tggccgtggt cgtctgactg cacgtcagat cgaagcagca cgtcgtgcta tgacccgtgc
     6481 agttaagcgt caaggtaaga tctggatccg tgtgttcccg gacaaaccga tcactgaaaa
     6541 gccgctggca gtgcgtatgg gtaaaggtaa aggtaacgtg gagtattggg ttgccttgat
     6601 tcagccgggt aaagtcctgt atgaaatgga cggtgttccg gaagagctgg cccgtgaagc
     6661 attcaagctg gcagcagcga aactgccgat taaaaccacc tttgtaacta agacggtgat
     6721 gtaatgaaag caaaagagct gcgtgagaag agcgttgaag agctgaacac cgagctgctg
     6781 aacctgctgc gtgagcagtt caacctgcgt atgcaggctg caagtggcca gctgcaacag
     6841 tctcacctgt tgaagcaagt gcgtcgcgat gtcgcacgcg ttaagacttt actgaacgag
     6901 aaggcgggtg cgtaatgacc gataaaatcc gtactctgca aggtcgcgtt gttagcgaca
     6961 aaatggagaa atccattgtt gttgctatcg aacgttttgt gaaacacccg atctacggta
     7021 aattcatcaa gcgtacgacc aaactgcacg tacatgacga gaacaacgaa tgcggtatcg
     7081 gtgacgtggt tgaaatccgc gaatgccgtc cgctgtccaa gactaaatcc tggacgctgg
     7141 ttcgcgttgt agagaaagcg gttctgtaat acagtacact ctctcgatac gaataaacgg
     7201 ctcagaaatg agccgtttat tttttctacc catatccttg aagcggtgtt ataatgccgc
     7261 gccctcgata tggggatttt taacgacctg attttcgggt ctcagtagta gttgacatta
     7321 gcggagcact aaaatgatcc aagaacagac tatgctgaac gtcgccgaca actccggtgc
     7381 acgtcgcgta atgtgtatca aggttctggg tggctcgcac cgtcgctacg caggcgtagg
     7441 cgatatcatc aagatcacca tcaaagaagc aattccgcgt ggtaaggtca aaaaaggtga
     7501 tgtgctgaag gcggtagtgg tgcgcaccaa gaagggtgtt cgtcgcccgg acggttctgt
     7561 cattcgcttc gatggtaatg cttgtgttct tctgaacaac aacagcgagc agcctatcgg
     7621 tacgcgtatt tttgggccgg taactcgtga gcttcgtagt gagaagttca tgaaaattat
     7681 ctctctggca ccagaagtac tctaaggagc gaatcatggc agcgaaaatc cgtcgtgatg
     7741 acgaagttat cgtgttaacc ggtaaagata aaggtaaacg cggtaaagtt aagaatgtcc
     7801 tgtcttccgg caaggtcatt gttgaaggta tcaatctggt taagaaacat cagaagccgg
     7861 ttccggccct gaaccaaccg ggtggcatcg ttgaaaaaga agccgctatt caggtttcca
     7921 acgtagcaat cttcaatgcg acaaccggca aggctgaccg tgtaggcttt agattcgaag
     7981 acggtaaaaa agtccgtttc ttcaagtcta acagcgaaac tatcaagtaa tttggagtag
     8041 tacgatggcg aaactgcatg attactacaa agacgaagta gttaaaaaac tcatgactga
     8101 gtttaactac aattctgtca tgcaagtccc tcgggtcgag aagatcaccc tgaacatggg
     8161 tgttggtgaa gcgatcgctg acaaaaaact gctggataac gcagcagcag acctggcagc
     8221 aatctccggt caaaaaccgc tgatcaccaa agcacgcaaa tctgttgcag gcttcaaaat
     8281 ccgtcagggc tatccgatcg gctgtaaagt aactctgcgt ggcgaacgca tgtgggagtt
     8341 ctttgagcgc ctgatcacta ttgctgtacc tcgtatccgt gacttccgtg gcctgtccgc
     8401 taagtctttc gacggtcgtg gtaactacag catgggtgtc cgtgagcaga tcatcttccc
     8461 agaaatcgac tacgataaag tcgaccgcgt tcgtggtttg gatattacca ttaccactac
     8521 tgcgaaatct gacgaagaag gccgcgctct gctggctgcc tttgacttcc cgttccgcaa
     8581 gtaaggtagg gttactaaat ggctaagcaa tcaatgaaag cacgcgaagt aaaacgcgta
     8641 gctttagctg ataaatactt cgcgaaacgc gctgaactga aagcgatcat ctctgatgtg
     8701 aacgcttccg acgaagatcg ttggaacgct gttctcaagc tgcagactct gccgcgtgat
     8761 tccagcccgt ctcgtcagcg taaccgctgc cgtcaaacag gtcgtccgca tggtttcctg
     8821 cggaagttcg ggttgagccg tattaaggtc cgtgaagccg ctatgcgcgg tgaaatcccg
     8881 ggtctgaaaa aggctagctg gtaattgtca ccaattgaat cacgggagta aagacagatg
     8941 agcatgcaag atccgatcgc ggatatgctg acccgtatcc gtaacggtca ggccgcgaac
     9001 aaagctgcgg tcaccatgcc ttcctccaag ctgaaagtgg caatcgccaa cgtgctgaag
     9061 gaagaaggtt ttattgaaga ttttaaagtt gaaggcgaca ccaagcctga actggaactt
     9121 actctgaagt atttccaggg caaagctgtt gtagaaagca ttcagcgtgt cagccgccca
     9181 ggtctgcgca tctataaacg taaagatgag ctgccgaaag ttatggcggg tctgggtatc
     9241 gcagttgttt ctacctctaa aggtgttatg actgatcgtg cagcgcgcca ggctggtctt
     9301 ggtggcgaaa ttatctgcta cgtagcctaa tcggaggaaa aaatgtctcg tgttgctaaa
     9361 gcaccggtcg ttgttcctgc cggcgttgac gtaaaaatca acggtcaggt tattacgatc
     9421 aaaggtaaaa acggcgagct gactcgtact ctcaacgatg ctgttgaagt taaacatgca
     9481 gataataccc tgaccttcgg tccgcgtgat ggttacgcag acggttgggc acaggctggt
     9541 accgcgcgtg ccctgctgaa ctcaatggtt atcggtgtta ccgaaggctt cactaagaag
     9601 ctgcagctgg ttggtgtagg ttaccgtgca gcggttaaag gcaatgtgat taacctgtct
     9661 ctgggtttct ctcatcctgt tgaccatcag ctgcctgcgg gtatcactgc tgaatgtccg
     9721 actcagactg aaatcgtgct gaaaggcgct gataagcagg tgatcggcca ggttgcagcg
     9781 gatctgcgcg cctaccgtcg tcctgagcct tacaaaggca agggtgttcg ttacgccgac
     9841 gaagtcgtgc gtaccaaaga ggctaagaag aagtaaggta acactatgga taagaaatct
     9901 gctcgtatcc gtcgtgcgac ccgcgcacgc cgcaagctcc aggagctggg cgcaactcgc
     9961 ctggtggtac atcgtacccc gcgtcacatt tacgcacagg taattgcacc gaacggttct
    10021 gaagttctgg tagctgcttc tactgtagaa aaagctatcg ctgaacaact gaagtacacc
    10081 ggtaacaaag acgcggctgc agctgtgggt aaagctgtcg ctgaacgcgc tctggaaaaa
    10141 ggcatcaaag atgtatcctt tgaccgttcc gggttccaat atcatggtcg tgtccaggca
    10201 ctggcagatg ctgcccgtga agctggcctt cagttctaag gtagaggtgt aagatggctc
    10261 acatcgaaaa acaagctggc gaactgcagg aaaagctgat cgcggtaaac cgcgtatcta
    10321 aaaccgttaa aggtggtcgt attttctcct tcacagctct gactgtagtt ggcgatggta
    10381 acggtcgcgt tggttttggt tacggtaaag cgcgtgaagt tccagcagcg atccagaaag
    10441 cgatggaaaa agcccgtcgc aatatgatta acgtcgcgct gaataacggc actctgcaac
    10501 accctgttaa aggtgttcac acgggttctc gcgtattcat gcagccggct tccgagggta
    10561 ccggtatcat cgccggtggt gcaatgcgcg ccgttctgga agtcgctggg gttcataacg
    10621 ttctggctaa agcgtatggt tccaccaacc cgattaacgt ggttcgtgca actatcgatg
    10681 gcctggaaaa tatgaattct ccagaaatgg tcgctgccaa gcgtggtaaa tccgttgaag
    10741 aaattctggg gaaataaacc atggcaaaga ctattaaaat tactcaaacc cgcagtgcaa
    10801 tcggtcgtct gccgaaacac aaggcaacgc tgcttggcct gggtctgcgt cgtattggtc
    10861 acaccgtaga gcgcgaggat actcctgcta ttcgcggtat gatcaacgcg gtttccttca
    10921 tggttaaagt tgaggagtaa gagatgcgtt taaatactct gtctccggcc gaaggctcca
    10981 aaaaggcggg taaacgcctg ggtcgtggta tcggttctgg cctcggtaaa accggtggtc
    11041 gtggtcacaa aggtcagaaa tctcgttctg gcggtggcgt acgtcgcggt ttcgagggtg
    11101 gtcagatgcc tctgtaccgt cgtctgccga aattcggctt cacttctcgt aaagcagcga
    11161 ttacagccga agttcgtctg tctgacctgg ctaaagtaga aggcggtgtt gtagacctga
    11221 acacgctgaa agcggctaac attatcggta tccagatcga gttcgcgaaa gtgatcctgg
    11281 ctggcgaagt aacgactccg gtaactgttc gtggcctgcg tgttactaaa ggcgctcgtg
    11341 ctgctatcga agctgctggc ggtaaaatcg aggaataagt agcagatggc taaacaaccg
    11401 ggattagatt ttcaaagtgc caaaggtggc ttaggcgagc tgaaacgcag actgctgttt
    11461 gttatcggtg cgctgattgt gttccgtatt ggctctttta ttccgatccc tggtattgat
    11521 gccgctgtac ttgccaaact gcttgagcaa cagcgaggca ccatcattga gatgtttaac
    11581 atgttctctg gtggtgctct cagccgtgct tctatctttg ctctggggat catgccgtat
    11641 atttcggcgt cgatcattat ccagctactg acggtggttc atccaacgtt ggcagaaatt
    11701 aagaaagaag gggagtctgg tcgtcgtaag atcagccagt acacccgcta cggtactctg
    11761 gtgctggcaa tattccagtc gatcggtatt gctactggtc tgccgaatat gcctggtatg
    11821 caaggcctgg tgattaaccc gggctttgca ttctacttca ccgctgttgt aagtctggtc
    11881 acaggaacca tgttcctgat gtggttgggc gaacagatca ctgaacgagg tatcggcaac
    11941 ggtatttcaa tcattatctt cgccggtatt gtcgcgggac tcccgccagc cattgcccat
    12001 actatcgagc aagcgcgtca aggcgacctg cacttcctcg tgttgctgtt ggttgcagta
    12061 ttagtatttg cagtgacgtt ctttgttgta tttgttgagc gtggtcaacg ccgcattgtg
    12121 gtaaactacg cgaaacgtca gcaaggtcgt cgtgtctatg ctgcacagag cacacattta
    12181 ccgctgaaag tgaatatggc gggggtaatc ccggcaatct tcgcttccag tattattctg
    12241 ttcccggcga ccatcgcgtc atggttcggg ggcggtactg gttggaactg gctgacaaca
    12301 atttcgctgt atttgcagcc tgggcaaccg ctttatgtgt tactctatgc gtctgcaatc
    12361 atcttcttct gtttcttcta cacggcgttg gttttcaacc cgcgtgaaac agcagataac
    12421 ctgaagaagt ccggtgcatt tgtaccagga attcgtccgg gagagcaaac ggcgaagtat
    12481 atcgataaag taatgacccg cctgaccttg gttggtgcgc tgtacattac ctttatctgc
    12541 ctgatcccgg agttcatgcg tgatgcaatg aaagtaccgt tctacttcgg tgggacctca
    12601 ctgcttatcg ttgttgtcgt gattatggac tttatggctc aagtgcaaac tctgatgatg
    12661 tccagtcagt atgagtctgc attgaagaag gcgaacctga aaggctacgg ccgataattg
    12721 gtcgcccgag aagttacgga gagtaaaaat gaaagttcgt gcttccgtca agaaattatg
    12781 ccgtaactgc aaaatcgtta agcgtgatgg tgtcatccgt gtgatttgca gtgccgagcc
    12841 gaagcataaa cagcgccaag gctgattttt tcgcatattt ttcttgcaaa gttgggttga
    12901 gctggctaga ttagccagcc aatcttttgt atgtctgtgc gtttccattt gagtatcctg
    12961 aaaacgggct tttcagcatg gaacgtacat attaaatagt aggagtgcat agtggcccgt
    13021 atagcaggca ttaacattcc tgatcataag catgccgtaa tcgcattaac ttcgatttat
    13081 ggcgtcggca agacccgttc taaagccatc ctggctgcag cgggtatcgc tgaagatgtt
    13141 aagatcagtg agctgtctga aggacaaatc gacacgctgc gtgacgaagt tgccaaattt
    13201 gtcgttgaag gtgatctgcg ccgtgaaatc agcatgagca tcaagcgcct gatggatctt
    13261 ggttgctatc gcggtttgcg tcatcgtcgt ggtctcccgg ttcgcggtca gcgtaccaag
    13321 accaacgcac gtacccgtaa gggtccgcgc aaaccgatca agaaataatc ggggtgattg
    13381 aataatggca aaggcaccaa ttcgtgcacg taaacgtgta agaaaacaag tctctgacgg
    13441 cgtggctcat atccatgctt ctttcaacaa caccatcgtg actatcactg atcgtcaggg
    13501 taacgcgttg ggttgggcaa cagccggtgg ttccggtttc cgtggttctc gcaaatccac
    13561 tccgtttgca gcacaggttg cagcagagcg ttgcgctgac gtcgtgaaag aatacggcat
    13621 caagaatctg gaagttatgg ttaaaggtcc gggtccaggc cgcgaatcta ctattcgtgc
    13681 tctgaacgcc gcaggtttcc gcatcactaa cattactgat gtgactccga tccctcataa
    13741 cggttgtcgt ccgccgaaaa aacgtcgcgt ataacgcctc gttttccagg tttgttggag
    13801 aaagaaaatg gcaagatatt tgggtcctaa gctcaagctg agccgtcgtg agggcaccga
    13861 cttattcctt aagtctggcg ttcgcgcgat cgataccaag tgtaaaattg aacaagctcc
    13921 tggccagcac ggtgcgcgta aaccgcgtct gtctgactat ggtgtgcagt tgcgtgaaaa
    13981 gcaaaaagtt cgccgtatct atggtgtgct ggagcgtcag ttccgtaact actacaaaga
    14041 agcagcacgt ctgaaaggca acaccggtga aaacctgttg gctctgctgg aaggtcgtct
    14101 ggacaacgtt gtataccgta tgggcttcgg tgccactcgt gcagaagcac gtcagctggt
    14161 tagccataaa gcaattatgg taaacggtcg tgttgttaac atcgcttctt atcaggttag
    14221 tccgaatgac gttgtaagca ttcgtgagaa agcgaagaag cagtctcgcg tgaaagccgc
    14281 tctggagctg gctgagcagc gtgaaaagcc aacctggctg gaagttgatg ctggcaagat
    14341 ggaaggtacg tttaagcgta agccggagcg ttctgatctg tctgcggaca ttaacgaaca
    14401 cctgatcgtc gagctttact ccaagtaaag cttagtacca aagagaggac acaatgcagg
    14461 gttctgtgac agagtttcta aaaccgcgcc tggttgatat cgagcaagtg agttcgacgc
    14521 acgccaaggt gacccttgag cctttagagc gtggctttgg ccatactctg ggtaacgcac
    14581 tgcgccgtat tctgctctca tcgatgccgg gttgcgcggt gaccgaggtt gagattgatg
    14641 gtgtactaca tgagtacagc accaaagaag gcgttcagga agatatcctg gaaatcctgc
    14701 tcaacctgaa agggctggcg gtgagagttc agggcaaaga tgaagttatt cttaccttga
    14761 ataaatctgg cattggccct gtgactgcag ccgatatcac ccacgacggt gatgtcgaaa
    14821 tcgtcaagcc gcagcacgtg atctgccacc tgaccgatga gaacgcgtct attagcatgc
    14881 gtatcaaagt tcagcgcggt cgtggttatg tgccggcttc tacccgaatt cattcggaag
    14941 aagatgagcg cccaatcggc cgtctgctgg tcgacgcatg ctacagccct gtggagcgta
    15001 ttgcctacaa tgttgaagca gcgcgtgtag aacagcgtac cgacctggac aagctggtca
    15061 tcgaaatgga aaccaacggc acaatcgatc ctgaagaggc gattcgtcgt gcggcaacca
    15121 ttctggctga acaactggaa gctttcgttg acttacgtga tgtacgtcag cctgaagtga
    15181 aagaagagaa accagagttc gatccgatcc tgctgcgccc tgttgacgat ctggaattga
    15241 ctgtccgctc tgctaactgc cttaaagcag aagctatcca ctatatcggt gatctggtac
    15301 agcgtaccga ggttgagctc cttaaaacgc ctaaccttgg taaaaaatct cttactgaga
    15361 ttaaagacgt gctggcttcc cgtggactgt ctctgggcat gcgcctggaa aactggccac
    15421 cggcaagcat cgctgacgag taaccggatc acaggttaag gttttactga gaaggataag
    15481 gtcatgcgcc atcgtaagag tggtcgtcaa ctgaaccgca acagcagcca tcgccaggct
    15541 atgttccgca atatggcagg ttcactggtt cgtcatgaaa tcatcaagac gactctgcct
    15601 aaagcgaaag agctgcgccg cgtagttgag ccgctgatta ctcttgccaa gactgatagc
    15661 gttgctaatc gtcgtctggc attcgcccgt actcgtgata acgagatcgt ggcaaaactg
    15721 tttaacgaac tgggcccgcg tttcgcgagc cgtgccggtg gttacactcg tattctgaag
    15781 tgtggcttcc gtgcaggcga caatgcgccg atggcttaca tcgagctggt tgatcgttca
    15841 gagaaagcag aagctgctgc agagtaatct gaagcaacgt aaaaaaaccc gccccggcgg
    15901 gtttttttat acccgtagca tccccactta tctacaatag ctgtactctt tttgttcatc
    15961 ccctggagta tttatgtggt tacttgacca gtgggcagag cgtcatatag cagaagcgca
    16021 agcgaaaggt gagtttgata acctggtagg tagcggcgaa ccattgatac tggatgatga
    16081 ttctcatgtg ccaccggaat tacgtgcggg gtatcgcttg ctgaagaatg ctggttgctt
    16141 accgccagaa cttgagcata ggagggaagc aatacagctt ctggatattc tcaaaggtat
    16201 ccgtcatgat gatccgcaat atcaagaggt tagtcgtcga ttgtcattac tggaattgaa
    16261 gctgcgacaa gctggattga gtaccgattt tttacgcggc gattatgctg acaagttgtt
    16321 gaacaaaatc aacgataact agtggagtat gtatgtatcg cattggtgag ctggcaaaaa
    16381 tggcggaagt aacacccgac acgattcgtt attacgaaaa gcagcagatg atggagcatg
    16441 aagtacgcac cgaaggtggg tttcgcctgt ataccgaaag cgatctccag cgattgaaat
    16501 ttatccgcca tgccagacaa ctaggtttca gtctggagtc gatccgcgag ttgctgtcga
    16561 tccgtatcga tcctgaacac catacctgtc aggagtcaaa aggcattgtg caggaaagat
    16621 tgcaggaagt cgaagctcgt atagccgagt tgcagagtat gcagcgttcc ttgcaacgcc
    16681 ttaatgatac ctgttgtggg accgcccata gcagtgttta ttgctcgatt cttgaagctc
    16741 ttgaacaagg ggcgagtggc gttaagagtg gctgttgatt ttttgcactg gcaggattac
    16801 actcgcgccg ttaaataacc aattggagtt tttatgagcc gatatcagca tactaaaggg
    16861 cagataaagg ataatgcgat agaagcatta ctacatgatc ccttattccg acagcgagta
    16921 gagaaaaata agaaagggaa aggcagttac atgcgaaaag gaaaacatgg caatcggggg
    16981 aactgggagg ccagtggcaa gaaagtaaat cactttttta ccactggcct tctgctttca
    17041 gacgcttgtt aagagcggtt attctgctct ttcagcaaat cacgaatttc tgtcagtaat
    17101 acttcttctt tagttggtgc aggtgttgct gctgctggtt cttctttttt ccgattcagt
    17161 ttgttgatta gcttaatcgc cataaagatg gcaaaggcca ctatcagaaa atcaaagacg
    17221 ttttgaatga agacaccgta atgcatcaca acagcaggga tatccccttg ggcctcgcgt
    17281 agcgtgacag caaactgttt aaagtcgatc ccgccaatta ataagcccag aggtggcatg
    17341 atgatatcgg caaccagtga agatacaatc ttcccgaatg ccgctccgat aatgacaccc
    17401 actgccaaat ccaccacgtt cccgcgcatc gcaaattcgc gaaattcttt aataatgctc
    17461 atgttattct ccctatgaag ccgacaacca taagtctaac aaatgttaag ccattttcct
    17521 gccaggaatg gatttaatta atccttaata atcaaaggga aataataagg cgtcattaga
    17581 cgccttatta attacaagaa gaaaggactt ggctggaaga gtctttcgac gtcggtaata
    17641 aactttttat ctgtgaggaa cataattacg tgatcgcctt gctcaatgcg cagattgtca
    17701 ttggcaatca tcacatcgtt tccacgtacc accgctccaa taatcgttcc tggcggtagc
    17761 ttgatttcgt caatgactct gccgacaacg cgtgaggtgc tttcatcacc gtgagcaacg
    17821 gcttcaatag cttctgctac gccgcggcgt aatgaggaaa caccaacaat atctgctttt
    17881 cgcacatggc taagcaacgc agaaatagtt gcttgttgtg gtgaaatcgc aatatcgatc
    17941 acgctcccct gaaccagatc cacataagcg cgacgttgga tcaataccat cacctttttc
    18001 gcacccatac gtttggcaag catggcggac atgatattgg cctcgtcatc gttggtgacg
    18061 gcaataaaca gatcaacttg atcgatatgt tcttcggcca gcagttcttg atccgacgca
    18121 tcaccaaaaa agacgatcgt attctgcaac ttttccgcca gttcggcagc gcgctgctga
    18181 ttacgttcga tgagtttaac gctgtaatct ttttccagac gacgcgccag ccctgcaccg
    18241 atattaccgc caccaaccag catgatccgc ttatacggtt tttccagtcg ctgtaattca
    18301 ctcatcaccg cacggatatg ctgtgaagcg gcaataaaga acacttcatc accagcttca
    18361 acaatggtcg aaccttgcgg acgaatgggg cgatcgtggc ggaaaattgc tgccacacga
    18421 gtatcgatat gtggcatatg ttcgcgcatg gtcgaaagtg cattaccaat cagcgggccg
    18481 ccataatagg ctttaaccac agccaggctg actttaccct cagcgaagtt caccacctgc
    18541 aatgcgccgg gatactcaat cagtcggtaa atattatcga taaccaactg ctctggtgcg
    18601 atcagatgat caatcggtac agcatctgaa tgaaatagtt tatcggcatc gcgcacgtag
    18661 tctggtgagc ggatacgagc gatgcgatta ggggtgttga aaagtgagta ggctacctgg
    18721 caggcaacca tattggtttc atctgaactg gttacagcaa ccagcatatc ggcgtcgtcg
    18781 gcacctgcct cccgcaatac gcgtggatga gagccatgcc cctgcacgac ccgcaggtca
    18841 aatttatcct gtaaggtccg cagacgctca ccgttggtat cgacaacagt aatatcgttg
    18901 ttctcgccaa ccaggttttc cgccagtgtg ccgccaacct ggccggcacc cagaatgata
    18961 attttcatca gtcgcgaccc gttatctcat cactttttga ttagcttagc gtaaaagaag
    19021 ccgtcgccct cttcggcacc gggcaaattt tgtttacccg gttgctctgg tgttcctgtt
    19081 tcacaaagtt cggcatcagc ggtacgttgc aaaaaggctt taatctgcag gctattttct
    19141 tccggtaaca ccgaacaggt ggcatagacc agggttccac cggattttaa atgtggccaa
    19201 atggcatcga gtatttcaga ctgcaattgc gcgagttccg ggatatcgcg atcacgacgt
    19261 aaccatttaa tatccggatg gcgacgaatc acccccgttg ctgaacaagg cgcatctaat
    19321 aaaatgcgat caaactgttg ctcgccacac cattgggaag ggttgcggcc atcaccttgt
    19381 ttcacggtcg ccttcatacc aaggcgtttt aaattgtcgt aaacgcgaga gaggcgctgc
    19441 tcatcaatat cgaccgccag aacttgcgcc tctggagcaa cctcaagaat atgcgttgtt
    19501 ttaccgcctg gggcggcgca aagatccaga atgtgttcac cattttgtgg agcaagccag
    19561 gtcatgcaac cttgtgctga agcatcctga acggtaaccc atccttcttc aaaaccgggt
    19621 agcgcatgaa caggtgcagg tgtttccagc tgtacagcat cagggtaatc cgcatgcggg
    19681 aaacctttca ttcctgcttc atccagcaat gcaagccagc tgtcgcggga atgatgcgta
    19741 cgattaacgc gcagccacat tggcggacgc tggttattgg cttcgacgat ggattgccac
    19801 tgctcgggat acgccttttg cagacgcttc agcaaccagg aaggatgcag ataacgtgca
    19861 tcactggcat taaactcggc taataactca tcttgctgac gctggaactg acgtaataca
    19921 ccgttaatca atcccttaag ttgcgggcgc ttaattgcaa ctgcgccttc aaccgtttca
    19981 gccagcgcgg catgaggtgg aatgcgggta taaagcagtt gatacaaacc aaccataatc
    20041 aggtaatgaa cagtacgctg tttgcctgtc atcggacggg ccattagctt attaatcagc
    20101 cagtctaact gtgagagcgt acgcagtacg ccaaagcata actcctggag aagagcttta
    20161 tctttatcgg aaactttttg ctggagcggt ggcagaatgt tgctcagtga ttgcccttgc
    20221 tcaacgactt gttcaatggc ctgggccgcc atgctacgta aattacgtt
//
LOCUS       DAHHTG010000078        19825 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.078, whole genome shotgun sequence.
ACCESSION   DAHHTG010000078 DAHHTG010000000
VERSION     DAHHTG010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19825)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19825)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19825
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.078"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            complement(17..163)
                     /locus_tag="NBI74_002916"
                     /pseudo
     CDS             complement(17..163)
                     /locus_tag="NBI74_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080164.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            217..399
                     /locus_tag="NBI74_002917"
     CDS             217..399
                     /locus_tag="NBI74_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein"
                     /protein_id="HCD1933211.1"
                     /translation="MNHPVIGVVTKADLASMEQISLVKSWLREAGAHNVLVTSAVNNN
                     GVTELFALLHTEEGCC"
     gene            complement(500..829)
                     /locus_tag="NBI74_002918"
     CDS             complement(500..829)
                     /locus_tag="NBI74_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF496 family protein"
                     /protein_id="HCD1933212.1"
                     /translation="METTKPSFQDVLEFVRLFRRKNKLQREIQDVEKKIRDNQKRVLL
                     LDNLSDYIKPGMSVEAIQGIIASMKGDYEDRVDDYIIKNAELSKERRDISKKLKAMGE
                     MKNGEAK"
     gene            complement(1001..2059)
                     /locus_tag="NBI74_002919"
     CDS             complement(1001..2059)
                     /locus_tag="NBI74_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HCD1933213.1"
                     /translation="MRADKSLSPFEIRVYRHYRIVHGTRVALAFLLTFLIIRLFTIPE
                     STWPLVTMVVIMGPISFWGNVVPRAFERIGGTVLGSILGLIALQLELISLPLMLVWCA
                     AAMFLCGWLALGKKPYQGLLIGVTLAIVVGSPTGEIDTALWRSGDVILGSLLAMLFTG
                     IWPQRAFIHWRIQLAKSLTEYNRVYQSAFSPNLLERPRLESHLQKLLTDAVKMRGLIA
                     PASKETRIPKSIYEGIQTINRNLVCMLELQINAYWATRPSHFVLLNAQKLRDTQHMMQ
                     QILLSLVHALYEGNPQPVFSNTEKLNDAVEELRQLLDNHHDLKVVETPIYGYVWLNME
                     TAHQLELLSNLICRALRK"
     gene            complement(2257..2730)
                     /gene="sbmC"
                     /locus_tag="NBI74_002920"
     CDS             complement(2257..2730)
                     /gene="sbmC"
                     /locus_tag="NBI74_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor SbmC"
                     /protein_id="HCD1933214.1"
                     /translation="MNYEIKQEDKRTVAGFHLVGPWEQTVKKGFEQLMMWIDSKNIVP
                     KEWVAVYYDNPDETPAEKLRCDTVVTVPNNFTLPENSEGVILTEISGGQYAVAVARVV
                     GDDFAKPWYQFFNSLLQDSAYEMLPKPCFEVYLNNGAEDGYWDIEMYVAVQPKHH"
     gene            complement(2849..4015)
                     /gene="dacD"
                     /locus_tag="NBI74_002921"
     CDS             complement(2849..4015)
                     /gene="dacD"
                     /locus_tag="NBI74_002921"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase DacD"
                     /protein_id="HCD1933215.1"
                     /translation="MKRRLIIAASLFVFNLSSGFAAENIPFSPQPPEIHAGSWVLMDY
                     TTGQILTAGNEHQQRNPASLTKLMTGYVVDRAIDSHRITPDDIVTVGRDAWAKDNPVF
                     VGSSLMFLKEGDRVSVRDLSRGLIVDSGNDACVALADYIAGGQRQFVEMMNNYAEKLH
                     LKDTHFETVHGLDAPGQHSSAYDLAVLSRAIIHGEPEFYHMYSEKSLTWNGITQQNRN
                     GLLWDKTMNVDGLKTGHTSGAGFNLIASAVDGQRRLIAVVMGADSAKGREEEARKLLR
                     WGQQNFTTVQILHRGKKVGTERIWYGDKENIALGTEQEFWMVLPKAEIPHIKAKYTLD
                     GKELTAPISAHQRVGEIELYDRDKQVAHWPLVTLESVGEGSMFSRLSDYFHHKA"
     gene            4224..5651
                     /gene="sbcB"
                     /locus_tag="NBI74_002922"
     CDS             4224..5651
                     /gene="sbcB"
                     /locus_tag="NBI74_002922"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease I"
                     /protein_id="HCD1933216.1"
                     /translation="MMNDGKQQSTFLFHDYETFGTHPALDRPAQFAAIRTDNEFNVIG
                     EPEVFYCKPADDYLPQPGAVLITGITPQEARAKGENEATFAARIHSLFTVPKTCILGY
                     NNVRFDDEVTRNVFYRNFYDPYAWSWQHDNSRWDLLDVMRACYALRPEGINWPENDDG
                     LPSFRLEHLTKANGIEHSNAHDAMADVYATIAMAKLVKTRQPRLFDYLFTHRNKHKLM
                     ALIDVPQMKPLVHVSGMFGAWRGNTSWVAPLAWHPENRNAVIMVDLAGDISPLLELDS
                     DTLRERLYTAKADLGDNAAVPVKLVHINKCPVLAQANTLRPEDADRLGINRQHCLDNL
                     KILRENPQVREKVVAIFAEAEPFTPSENVDAQLYNGFFSDADRAAMKIVLETEPRNLP
                     ALDITFVDKRIEKLLFNYRARNFPGTLDYAEQQRWLEHRRQVFTPEFLQGYADELQML
                     AQQYADDKEKVALLKALWQYAEEIV"
     gene            complement(5725..7083)
                     /gene="plaP"
                     /locus_tag="NBI74_002923"
     CDS             complement(5725..7083)
                     /gene="plaP"
                     /locus_tag="NBI74_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416518.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PlaP"
                     /protein_id="HCD1933217.1"
                     /translation="MSHNVTPNTSRVELRKTLTLVPVVMMGLAYMQPMTLFDTFGIVS
                     GLTDGHVPTAYAFALIAILFTALSYGKLVRRYPSAGSAYTYAQKSISPTVGFMVGWSS
                     LLDYLFAPMINILLAKIYFEALVPSIPSWMFVVALVAFMTAFNLRSLKSVANFNTVIV
                     VLQIVLIAVILGMVVYGVFEGEGAGTLASTRPFWSGDAHVIPMITGATILCFSFTGFD
                     GISNLSEETKDAERVIPRAIFLTALIGGMIFIFATYFLQLYFPDISRFKDPDASQPEI
                     MLYVAGKAFQVGALIFSTITVLASGMAAHAGVARLMYVMGRDGVFPKSFFGYVHPKWR
                     TPAMNIILVGAIALLAINFDLVMATALINFGALVAFTFVNLSVISQFWIREKRNKTLK
                     DHFQYLFLPMCGALTVGALWVNLEESSMVLGLIWAAIGLIYLACVTKSFRNPVPQYED
                     VA"
     gene            complement(7073..7135)
                     /gene="yoeI"
                     /locus_tag="NBI74_002924"
     CDS             complement(7073..7135)
                     /gene="yoeI"
                     /locus_tag="NBI74_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YoeI"
                     /protein_id="HCD1933218.1"
                     /translation="MGQFFAYATVITVKENDHVA"
     gene            complement(7350..8279)
                     /locus_tag="NBI74_002925"
     CDS             complement(7350..8279)
                     /locus_tag="NBI74_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCD1933219.1"
                     /translation="MKPLLDVLMILDALEKEGSFAAASAKLYKTPSALSYTVHKLESD
                     LNIQLLDRSGHRAKFTRTGKMLLEKGREVLHTVRELEKQAIKLHEGWENELVIGVDDT
                     FPFSLLAPLIEAFYQHHSVTRLKFINGVLGGSWDALTQGRADIIVGAMHEPPSSSEFG
                     FSRLGDLEQVFAVAPHHPLAQEEEPLNRRIIKRYRAIVVGDTAQAGASTASQLLDEQE
                     AITVFDFKTKLELQISGLGCGYLPRYLAQRFLDSGALIEKKVVAQTLFEPVWIGWNEQ
                     TAGLASGWWRDEILANSAIAGVYAKSDDGKSAI"
     gene            complement(8325..9149)
                     /locus_tag="NBI74_002926"
     CDS             complement(8325..9149)
                     /locus_tag="NBI74_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCD1933220.1"
                     /translation="MKKVAIVGLGWLGMPLAMSLSARGWQVTGSKTTQDGVEAARMSG
                     IDSYLLRMEPELVCDSDDLDALMDADALVITLPARRSGPGDEFYLQAVQELVDSALAH
                     RIPRIIFTSSTSVYGDAQGTVKETTPRNPVTNSGRVLEELEDWLHNLPGTSVDILRLA
                     GLVGPGRHPGRFFAGKTAPDGEHGVNLVHLEDVIGAITLLLQAPKGGHIYNICAPAHP
                     ARNVFYPQMARLLGLEPPQFRNSLDSGKGKIIDGSRICNELGFEYQYPDPLVMPLE"
     gene            complement(9232..9486)
                     /gene="yoeB"
                     /locus_tag="NBI74_002927"
     CDS             complement(9232..9486)
                     /gene="yoeB"
                     /locus_tag="NBI74_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YoeB"
                     /protein_id="HCD1933221.1"
                     /translation="MKLIWSEESWDDYLYWQETDKRIVKKINEIIKDTRRTPFEGKGK
                     PEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAACRYHY"
     gene            complement(9483..9734)
                     /gene="yefM"
                     /locus_tag="NBI74_002928"
     CDS             complement(9483..9734)
                     /gene="yefM"
                     /locus_tag="NBI74_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoeB-YefM toxin-antitoxin system antitoxin YefM"
                     /protein_id="HCD1933222.1"
                     /translation="MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEE
                     YNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKDIIE"
     gene            10017..10067
                     /gene="hisL"
                     /locus_tag="NBI74_002929"
     CDS             10017..10067
                     /gene="hisL"
                     /locus_tag="NBI74_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="his operon leader peptide"
                     /protein_id="HCD1933223.1"
                     /translation="MTRVQFKHHHHHHHPD"
     misc_feature    10043..10166
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00514"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="His leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00514"
     gene            10213..11112
                     /gene="hisG"
                     /locus_tag="NBI74_002930"
     CDS             10213..11112
                     /gene="hisG"
                     /locus_tag="NBI74_002930"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HCD1933224.1"
                     /translation="MTDNTRLRIAMQKSGRLSDDSRELLARCGIKINLHTQRLIAMAE
                     NMPIDILRVRDDDIPGLVMDGVVDLGIIGENVLEEELLNRRAQGEDPRYFTLRRLDFG
                     GCRLSLATPVDEAWDGPLSLNGKRIATSYPHLLKRYLDQKGISFKSCLLNGSVEVAPR
                     AGLADAICDLVSTGATLEANGLREVEVIYRSKACLIQRDGEMEESKQQLIDKLLTRIQ
                     GVIQARESKYIMMHAPTERLDEVIALLPGAERPTILPLAGDQQRVAMHMVSSETLFWE
                     TMEKLKALGASSILVLPIEKMME"
     gene            11118..12422
                     /gene="hisD"
                     /locus_tag="NBI74_002931"
     CDS             11118..12422
                     /gene="hisD"
                     /locus_tag="NBI74_002931"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HCD1933225.1"
                     /translation="MSFNTIIDWNSCTAEQQRQLLMRPAISASESITRTVNDILDNVK
                     TRGDEALREYSAKFDKTTVTALKVSADEIAAASERLSDELKQAMAVAVKNIETFHTAQ
                     KLPPVDVETQPGVRCQQVTRPVASVGLYIPGGSAPLFSTVLMLATPARIAGCKKVVLC
                     SPPPIADEILYAAQLCGVQDVFNVGGAQAIAALTFGTESVPKVDKIFGPGNAFVTEAK
                     RQVSQRLDGAAIDMPAGPSEVLVIADSGATPDFVASDLLSQAEHGPDSQVILLTPDAD
                     MARRVAEAVERQLAELPRAETARQALNASRLIVTKDLAQCVEISNQYGPEHLIIQTRN
                     ARELVDGITSAGSVFLGDWSPESAGDYASGTNHVLPTYGYTATCSSLGLADFQKRMTV
                     QELSKEGFSVLASTIETLAAAERLTAHKNAVTLRVNALKEQA"
     gene            12419..13489
                     /gene="hisC"
                     /locus_tag="NBI74_002932"
     CDS             12419..13489
                     /gene="hisC"
                     /locus_tag="NBI74_002932"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HCD1933226.1"
                     /translation="MSTVTITDLARENVRNLTPYQSARRLGGNGDVWLNANEYPTAVE
                     FQLTQQTLNRYPECQPKAVIENYAQYAGVKPEQVLVSRGADEGIELLIRAFCEPGKDA
                     ILYCPPTYGMYSVSAETIGVECRTVPTLDNWQLDLQGISDKLDGVKVVYVCSPNNPTG
                     QLINPQDFRTLLELTRGKAIVVADEAYIEFCPQASLAGWLAEYPHLAILRTLSKAFAL
                     AGLRCGFTLANEEVINLLMKVIAPYPLSTPVADIAAQALSPQGIVAMRERVAQIIAER
                     EYLIAALKEIPCVEQVFDSETNYILARFKASSAVFKSLWDQGIILRDQNKQPSLSGCL
                     RITVGTREESQRVIDALRAEQV"
     gene            13489..14556
                     /gene="hisB"
                     /locus_tag="NBI74_002933"
     CDS             13489..14556
                     /gene="hisB"
                     /locus_tag="NBI74_002933"
                     /EC_number="3.1.3.15"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416526.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     histidinol-phosphatase/imidazoleglycerol-phosphate
                     dehydratase HisB"
                     /protein_id="HCD1933227.1"
                     /translation="MSQKYLFIDRDGTLISEPPSDFQVDRFDKLAFEPGVIPELLKLQ
                     KAGYKLVMITNQDGLGTQSFPQADFDGPHNLMMQIFTSQGVQFDEVLICPHLPADECD
                     CRKPKVKLVERYLAEQAMDRANSYVIGDRATDIQLAENMGITGLRYDRETLNWPMIGE
                     QLTRRDRYAHVVRNTKETQIDVQVWLDREGGSKINTGVGFFDHMLDQIATHGGFRMEI
                     NVKGDLYIDDHHTVEDTGLALGEALKIALGDKRGICRFGFVLPMDECLARCALDISGR
                     PHLEYKAEFTYQRVGDLSTEMIEHFFRSLSYTMGVTLHLKTKGKNDHHRVESLFKAFG
                     RTLRQAIRVEGDTLPSSKGVL"
     gene            14556..15146
                     /gene="hisH"
                     /locus_tag="NBI74_002934"
     CDS             14556..15146
                     /gene="hisH"
                     /locus_tag="NBI74_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HCD1933228.1"
                     /translation="MNVVILDTGCANLNSVKSAIARHGYEPKVSRDPDVVLLADKLFL
                     PGVGTAQAAMDQVRERELFDLIKACTQPVLGICLGMQLLGRRSEESNGVDLLGIIDED
                     VPKMTDFGLPLPHMGWNRVYPQAGNRLFQGIEDGAYFYFVHSYAMPVNPWTIAQCNYG
                     EPFTAAVQKDNFYGVQFHPERSGAAGAKLLKNFLEM"
     gene            15146..15883
                     /gene="hisA"
                     /locus_tag="NBI74_002935"
     CDS             15146..15883
                     /gene="hisA"
                     /locus_tag="NBI74_002935"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HCD1933229.1"
                     /translation="MIIPALDLIDGTVVRLHQGDYGKQRDYGNDPLPRLQDYAAQGAE
                     VLHLVDLTGAKDPAKRQIPLIKTLVAGVNVPVQVGGGVRSEEDVAALLEAGVARVVVG
                     STAVKSPDVVKGWFERFGADALVLALDVRIDEQGNKQVAVSGWQENSGVSLEQLVETY
                     LPVGLKHVLCTDISRDGTLAGSNVSLYEEVCARYPQVAFQSSGGIGDINDVAALRGTG
                     VRGVIVGRALLEGKFTVKEAIACWQNA"
     gene            15865..16641
                     /gene="hisF"
                     /locus_tag="NBI74_002936"
     CDS             15865..16641
                     /gene="hisF"
                     /locus_tag="NBI74_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HCD1933230.1"
                     /translation="MLAKRIIPCLDVRDGQVVKGVQFRNHEIIGDIVPLAKRYAEEGA
                     DELVFYDITASSDGRVVDKSWVSRVAEVIDIPFCVAGGIKSLEDAAKILSFGADKISI
                     NSPALADPTLITRLADRFGVQCIVVGIDTWYDAETGKYHVNQYTGDESRTRVTQWETL
                     DWVEEVQKRGAGEIVLNMMNQDGVRNGYDLKQLKKVREVCHVPLIASGGAGTMEHFLE
                     AFRDADVDGALAASVFHKQIINIGELKAYLATQGVEIRIC"
     gene            16635..17246
                     /locus_tag="NBI74_002937"
     CDS             16635..17246
                     /locus_tag="NBI74_002937"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HCD1933231.1"
                     /translation="MLTEQQRRELDWEKTDGLMPVIVQHAVSGEVLMLGYMTPEALDR
                     TIESGKVTFFSRTKQRLWTKGETSGNFLNVVSIAPDCDNDTLLVLANPIGPTCHKGTS
                     SCFGDTAHQWLFLYQLEQLLAERKSADPETSYTAKLYASGTKRIAQKVGEEGVETALA
                     ATVHDRFELTNEASDLMYHLLVLLQDQDLDLTTVIENLRKRHQ"
     gene            complement(17432..18409)
                     /gene="wzzB"
                     /locus_tag="NBI74_002938"
     CDS             complement(17432..18409)
                     /gene="wzzB"
                     /locus_tag="NBI74_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310855.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen chain length determinant protein
                     WzzB"
                     /protein_id="HCD1933232.1"
                     /translation="MRVENNNVSGQNHDPEQIDLIDLLVQLWRGKMTIIISVIVAIAL
                     AIGYLAVAKEKWTSTAIITQPDVGQIAGYNNAMNVIYGQAAPKVSDLQETLIGRFSSA
                     FSALAETLDNQEEPEKLTIEPSVKNQQLPLTVSYVGQTAEGAQMKLAQYIQQVDDKVN
                     QELEKDLKDNIALGRKNLQDSLRTQEVVAQEQKDLRIRQIQEALQYANQAQVTKPQIQ
                     QTQDVTQDTMFLLGSEALESMIKHEATRPLVFSSNYYQTRQNLLDIDNLDVDKLDIHA
                     YRYVMKPTLPIRRDSPKKAITLILAVLLGGMVGAGIVLGRNALRNYNAK"
     gene            complement(18555..19721)
                     /gene="ugd"
                     /locus_tag="NBI74_002939"
     CDS             complement(18555..19721)
                     /gene="ugd"
                     /locus_tag="NBI74_002939"
                     /EC_number="1.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 6-dehydrogenase"
                     /protein_id="HCD1933233.1"
                     /translation="MKITISGTGYVGLSNGLLIAQNHEVVALDILPSRVAMLNDRISP
                     IVDKEIQQFLQSDKIHFNATLDKNEAYRDADYVIIATPTDYDPKTNYFNTSSVESVIK
                     DVVEINPYAVMVIKSTVPVGFTAAMHKKYRTENIIFSPEFLREGKALYDNLHPSRIVI
                     GERSERAERFAALLQEGAIKQNIPMLFTDSTEAEAIKLFANTYLAMRVAYFNELDSYA
                     ESLGLNSRQIIEGVCLDPRIGNHYNNPSFGYGGYCLPKDTKQLLANYQSVPNNLISAI
                     VDANRTRKDFIADAILSRKPQVVGIYRLIMKSGSDNFRASSIQGIMKRIKAKGVEVII
                     YEPVMKEDSFFNSRLERDLATFKQQADVIISNRMAEELKDVADKVYTRDLFGSD"
BASE COUNT         5045 a         5084 c         4935 g         4761 t
ORIGIN      
        1 agcaccgtat tgacgcttat ttattggtga gaactacgtt ccatggcagg agttcgtcaa
       61 cacggttgga gggccattcc ggcagtatgc tcagaatatg gcgcagatac gcttccggat
      121 cgataccgtt cagacggcag gtgcctgctt ttccggcaac aagcaggaat ctcagcgctg
      181 aagcaggaat tagaggtaaa aacgccctac cgggccatga atcacccggt cattggagta
      241 gtaaccaaag cagatttagc cagcatggaa caaatatctt tggtgaaaag ttggttacgg
      301 gaagctggag cgcacaacgt gttagtaacc agtgcagtta ataacaatgg agttacagaa
      361 ctttttgcct tgctgcatac agaagaaggc tgttgttaat ttcgattggt tctcagatga
      421 gaatgatgac ggtgatacac agataagaat caatgagcaa tgagggttgc cgggcaaccc
      481 tcattgaata aaacgggaat tacttcgctt cgccgttttt catttcgccc atagctttca
      541 gctttttgga gatatcgcgg cgttctttgg agagctcggc atttttgatg atgtaatcgt
      601 caacgcgatc ttcatagtca cctttcatgc tggcgatgat gccctggatt gcttcaacgc
      661 tcatccctgg cttgatgtaa tcgctcaggt tgtccagcaa caggacgcgc ttctggttgt
      721 cacggatctt tttctcaacg tcctgaattt cacgttgcag tttgttctta cgacggaaca
      781 gacgaacaaa ttccagtacg tcctggaatg aaggcttggt agtttccatt tttatacccc
      841 tgataatgtg agagtcggat tcgtttaagc aaccgctatt cgttagggcc aacattactc
      901 atagctgccg cagatgacaa tgcttttatc ccttttcact atcataccct ttatccttgc
      961 tgaatcgaag cagcagcaag atgattctga agttcaggaa ttatttgcgc aaggcccggc
     1021 aaatcagatt cgatagcaac tcaagctgat gtgccgtttc catgttcagc cacacataac
     1081 catagattgg tgtttccacg actttcaggt catggtggtt atcgagcaac tgacgcagct
     1141 cttccacagc atcgttcaat ttttccgtat tggaaaaaac cggctgcgga ttaccttcgt
     1201 acagcgcatg aacaaggctc agcagtattt gctgcatcat gtgctgggta tcacgaagtt
     1261 tttgcgcgtt caataacacg aaatggctgg ggcgcgtggc ccagtatgca ttgatttgca
     1321 actccagcat acaaaccaga ttgcgattaa tggtctggat accttcatat atcgatttgg
     1381 gaatacgggt ttctttgctg gcgggcgcaa tcagcccacg cattttcacg gcatcggtca
     1441 ggagtttttg tagatggctt tccagacgtg ggcgttcgag taagttcggt gagaatgcag
     1501 attgatagac ccgattatac tcggtcagac ttttcgccag ttgaatgcgc cagtggatga
     1561 acgcccgttg tggccagata ccggtaaaca acattgccag taaagagccg aggatcacat
     1621 cgccgcttcg ccataacgcc gtatcaattt cacctgtcgg ggaacccaca acaattgcca
     1681 gcgtcacccc aatcaataat ccttgatacg gtttcttgcc cagcgccagc caaccgcaaa
     1741 gaaacatcgc cgccgcgcac cagactaaca tcagcggtaa cgaaattaac tccagttgca
     1801 gagcgataag acctaaaatc gaccccaaca ccgtaccgcc aatacgttcg aaggcacgag
     1861 ggacaacgtt cccccagaac gagattggcc ccataatcac caccatggtg accagcggcc
     1921 aggtgctttc cgggatagta aacaggcgga taatgagaaa agtgagcagg aatgccagcg
     1981 cgacccgagt accatgcaca atgcggtaat ggcggtatac ccggatttca aacgggctta
     2041 atgacttatc ggcacgcacg cagaaactcc gtaatgaaga ttaaaagcgc tcataacaca
     2101 tctgtcggcc agaaggcgcg cctctgagtt atccgacgga cataccggga acagtgtaat
     2161 ttcccggcag gagcgaaaaa tttgaaaggc gcatcttact ctttccgctt tcaaaaaaag
     2221 agtggtcttc gcgttaacac accgccctga gatgaattag tgatgttttg gctgcaccgc
     2281 aacatacatt tcgatatccc agtacccatc ttccgcgcca ttgttcaagt aaacctcgaa
     2341 gcagggcttt ggtaacattt cataagcact gtcctgcaag aggctattaa agaactgata
     2401 ccagggttta gcaaaatcat caccgactac acgagccacc gccaccgcat actgaccacc
     2461 tgaaatttct gtcagaatga cgccctcact gttttcgggg agcgtaaagt tattcggtac
     2521 cgtcacgacg gtgtcgcagc gtaatttttc ggcgggtgtt tcatctggat tgtcgtaata
     2581 gacagcaacc cactccttcg gcacaatatt tttgctatct atccacatca tcaactgctc
     2641 aaaacctttc tttaccgtct gctcccacgg gccaacaaga tggaaacctg caacggtacg
     2701 tttatcttcc tgcttaatct cgtagttcat gacgcctcca ctaatactgt ttttatatac
     2761 agtatagttg caatattaaa accacaagga atgagtgttg attatgcgag cagactcgca
     2821 ctcctgccag tctgctgcaa aagaaaggtc aggccttatg gtggaaataa tcactcaggc
     2881 gggaaaacat gctgccttcc ccgacagatt ccagggtaac cagcggccag tgcgccacct
     2941 gtttatcacg gtcgtaaagt tcaatttccc ctacccgctg atgggcgcta attggcgcgg
     3001 tgagttcttt accatcaagg gtatatttgg ctttgatatg tggaatttcg gctttcggta
     3061 gcaccatcca gaactcttgt tccgttccca gggcgatatt ttctttatcg ccataccaga
     3121 tgcgttccgt accgaccttt ttcccccggt gcaaaatttg caccgtagta aagttttgtt
     3181 gcccccaacg cagtaatttt cttgcctctt cctcacgacc ttttgcactg tcagccccca
     3241 ttaccactgc aatgagacga cgctgcccat ctacagccga agcaatgaga ttaaacccag
     3301 caccagaagt atgacccgtt ttcaggccgt caacattcat ggttttatcc cacaataacc
     3361 cgttacggtt ttgctgggtg ataccgttcc aggtgaggct tttctcactg tacatatgat
     3421 aaaactcggg ctcgccgtgg atgatagcgc gagaaagcac ggctaaatca taagccgagc
     3481 tatgctggcc aggtgcatcc agaccatgca ctgtttcaaa atgcgtatcc ttgagatgca
     3541 gcttctcggc atagttgttc atcatttcaa caaactgccg ttgcccaccg gcaatatagt
     3601 cagccagagc aacacaagcg tcatttccgg aatccacaat taaaccacgg cttaaatcac
     3661 gtaccgatac gcgatcgccc tctttcaaaa acatcagtga agaaccgaca aacaccggat
     3721 tatctttcgc ccacgcatca cgccccacgg tgacaatatc gtctggcgta atgcgatgac
     3781 tatcgatagc gcgatccacg acatagcccg tcatcagctt tgtcaggctg gcgggattgc
     3841 gctgttgatg ctcattaccc gcggtgagaa tctgaccggt ggtgtaatcc atcaataccc
     3901 aggacccggc atgaatctct ggaggctgag gtgaaaaagg aatgttttcc gccgcaaaac
     3961 cagacgataa attaaaaacg aacaaagaag cagcaataat aagacggcgt ttcaacagca
     4021 aaccctcagg tgtttcaaat agctgttctt tttacggaaa tacttatgaa ctggctggaa
     4081 taaagtgcaa gaaaatgtga ctactatctc atttttatct gacatgatct gttgccactc
     4141 gctgccaaat tgtcgcgcta aagctgatta gcacggtgat atttgatact ctggcagaca
     4201 gcagaaataa cggatttaac ctaatgatga atgacggtaa gcaacaatct acctttttgt
     4261 ttcacgatta cgaaaccttt ggaacgcacc ccgcgttaga tcgccctgca cagttcgcag
     4321 ccattcgcac tgataacgaa ttcaatgtca tcggcgaacc cgaagtcttt tactgcaagc
     4381 ccgcggatga ctatttaccc cagcctggag cagtattaat taccggtatt accccgcagg
     4441 aagcacgggc gaaaggagaa aacgaagcca cgtttgccgc ccgtattcac tcgcttttta
     4501 ccgtaccgaa gacctgtatt ctgggctaca acaatgtgcg tttcgacgac gaagtcacac
     4561 gcaacgtttt ttatcgtaat ttctacgatc cttacgcctg gagctggcag catgataact
     4621 cgcgctggga tttactggat gttatgcgtg cctgttatgc cctacgcccg gaaggaataa
     4681 actggcctga aaatgatgac ggtctaccga gctttcgcct tgagcattta accaaagcga
     4741 atggtattga acatagcaac gcccacgatg cgatggctga tgtgtacgcc actattgcga
     4801 tggcgaaact ggtaaaaacg cgtcagccac gcctgtttga ttatctcttt acccatcgta
     4861 ataaacacaa actgatggcg ttgattgatg ttccgcagat gaaacccctg gttcacgttt
     4921 ccggaatgtt tggggcatgg cgcggcaata ccagctgggt ggcaccgctg gcgtggcatc
     4981 ctgaaaatcg caatgccgta attatggtgg atttggcagg agacatttcg ccattactgg
     5041 agctggatag cgacacattg cgcgagcgtt tatataccgc aaaagccgat cttggcgata
     5101 acgccgccgt tccggttaag ctggtgcata tcaataaatg tccggtactg gcccaggcga
     5161 atacgctacg cccggaagat gccgaccgac tgggaattaa tcgtcagcat tgcctcgata
     5221 acctaaaaat tctgcgtgaa aatccgcaag tgcgtgaaaa agtggtggcg atattcgcgg
     5281 aagccgaacc gtttacgcct tcagagaacg tggatgcaca gctttataat ggctttttca
     5341 gtgacgccga tcgtgcagca atgaaaattg tgctggaaac cgagccgcgt aatttaccgg
     5401 cactggatat cacttttgtt gataaacgaa ttgaaaaact gttgttcaat tatcgagcac
     5461 gcaacttccc ggggacgctg gattatgccg agcagcaacg ctggctggag caccgccgcc
     5521 aggtcttcac gccagagttt ttgcagggct acgctgatga attgcagatg ctggcacaac
     5581 agtatgccga tgacaaagag aaagtggcgc tgttaaaagc gctttggcag tacgcggaag
     5641 agatcgtctg aaacaaaaaa gccggagcga caaactccgg ctttttgaga tttaccggat
     5701 gattatgtca tccggtttgt aagattacgc tacgtcttcg tactgcggaa ccgggttacg
     5761 gaaactcttg gttacgcaag ccaggtaaat cagaccgata gcagcccaga tcagacccag
     5821 aaccattgag ctttcttcca ggttaaccca cagcgcacca acggtcagcg caccacacat
     5881 cggcaggaac agatactgga agtgatcttt cagcgttttg ttacgcttct cacggatcca
     5941 gaactgcgag atgaccgaca ggttaacgaa ggtgaacgcc accaacgcac caaagttaat
     6001 cagcgccgta gccattacca ggtcgaagtt gattgccagc agggcaatcg cgccaaccag
     6061 aatgatgttc atcgccggag tacgccattt cgggtgtaca taaccgaaga agcttttcgg
     6121 gaacacgccg tcacgaccca ttacgtacat cagacgcgct acgcctgcgt gcgccgccat
     6181 accggacgcc agtacggtaa tggtggagaa gatcagcgcg ccaacctgga acgctttacc
     6241 cgcaacgtac agcatgattt caggctgtga cgcatccgga tctttaaaac gagagatatc
     6301 cgggaagtac agctgcagga agtaagttgc aaagatgaag atcatgccgc cgatcagcgc
     6361 ggtcaggaaa atcgcacgcg ggatcacgcg ctctgcatct ttggtttctt ccgacaggtt
     6421 gctgatgccg tcaaaaccgg taaaggagaa gcacaggatt gtcgccccgg taatcatcgg
     6481 gataacatgc gcatcgccag accagaacgg acgagtgctc gccagcgtac cagcgccttc
     6541 accttcaaat acgccataaa ccaccatgcc cagaatcacc gcgatcagca ctatctgcaa
     6601 cacgacgatt acagtgttga agttcgctac ggatttcaga ctacgcaggt taaaggcggt
     6661 cataaaggcc accagcgcca ccacaaacat ccacgatgga atggaaggca ccagagcttc
     6721 aaaatagatt ttcgccagca gaatgttgat catcggcgcg aacagatagt cgagcagaga
     6781 agaccaaccc accataaagc cgacagtcgg actaatggat ttctgggcgt aagtatatgc
     6841 agagccagca gaaggatagc ggcgaaccag cttcccgtag ctcagagccg taaacaggat
     6901 cgcaatcaat gcgaacgcat aggctgtcgg cacatgacca tccgtgaggc ctgaaacgat
     6961 accaaatgta tcaaacagcg tcatcggctg catataggct agacccatca ttacaaccgg
     7021 aactaacgta agcgttttac gcaattccac gcgagaggtg tttggagtaa cgttatgcga
     7081 catggtcatt ctcctttacg gtgataaccg tcgcgtaagc aaaaaattgc cccatttttt
     7141 tggattcctc agcgacaaca actgtcgatt tttagtaaat atctatccgg tacgaagccc
     7201 ggcctcttgg tatgaatgat tggtttgaag caaaaaataa ccgacgctaa ttaaacgtcg
     7261 gtttttagtc attttttgac agcggcgcat tgtgcctaaa tgggggggaa atgacaagag
     7321 aatgagaggc ttgtcataat aatttttctt taaatggctg attttccgtc atcagatttt
     7381 gcataaacac cggcgatcgc actatttgct aaaatttcat ctcgccacca gccgctggca
     7441 agccctgcgg tctgttcatt ccagccaatc cagacaggtt caaagagagt ttgggcgacc
     7501 actttcttct cgattaacgc gccactatcg agaaaacgtt gcgccagata acggggtaaa
     7561 tagccgcagc caaggccgct aatttgcagc tccagcttgg ttttaaaatc gaaaacggta
     7621 atggcttctt gttcgtcgag aagttgtgag gccgtagaag cgcctgcctg cgcagtgtcc
     7681 cccaccacaa tcgcccgata tcgtttaatg atacgacggt ttaacggttc ttcttcctgg
     7741 gctaacggat gatgaggcgc gaccgcaaag acttgttcaa gatcgcccaa ccgcgagaaa
     7801 ccaaactcac tggaagatgg tggctcatgc atcgcgccaa cgataatatc ggctcgcccc
     7861 tgagtcaggg catcccagga accaccgagt acgccattga taaacttcag gcgcgtcacg
     7921 ctatgatgtt gataaaaagc ttcaatgaga ggcgcaagca gagaaaaagg aaacgtgtcg
     7981 tccacgccaa tcaccagctc gttttcccag ccttcatgca gtttgatcgc ctgcttttcc
     8041 agttctcgca cggtatgcag aacttcccgc cctttctcta ataacatttt tccggtacgg
     8101 gtgaatttag cgcggtgtcc gctacgatca agcaattgaa tattaaggtc gctttccagc
     8161 ttgtgaacgg tgtaactcag agcggacggc gtcttgtaga gtttggctga cgccgccgca
     8221 aaactgcctt ctttttctaa ggcatcgagg atcatcaaca catccagcag tggtttcata
     8281 cacgccccct tgcagtgcat gcggcgtcct gctggcgtga tcgtttactc cagcggcatt
     8341 accagcggat cgggatactg gtattcaaat cccagttcat tacaaatccg actgccatca
     8401 ataatcttac ctttgccgct gtccagacta tttctgaact gcggaggttc cagccccaat
     8461 aaacgggcca tctgcggata gaaaacatta cgcgcagggt gagctggcgc acatatatta
     8521 tagatgtgtc cgcctttagg tgcctgtaac aacagagtga tagcgccaat aacatcttct
     8581 aaatggacta aattaacacc atgttcacca tcaggggcgg tttttccggc aaagaagcgt
     8641 ccgggatgac gtcccggtcc gaccaggccc gcaagacgca gaatatcgac cgaagtaccg
     8701 ggtaaattgt gcagccagtc ttcgagttct tctaatactc gtccactgtt ggtcaccgga
     8761 ttacgcgggg tggtttcttt caccgtgcct tgcgcgtcgc catagacaga tgtcgagctg
     8821 gtaaaaataa tgcggggaat acgatgggcc agcgcgctat ccactaactc ttgtaccgct
     8881 tgtaaataga actcatcgcc ggggccgcta cgacgtgccg gaagcgtaat gaccagcgca
     8941 tcggcatcca taagggcatc cagatcgtca gaatcgcaaa ctaactcagg ctccatgcga
     9001 agcagatagc tatcaatgcc actcattcgg gccgcttcga caccatcttg tgtggtttta
     9061 ctcccggtga cttgccagcc tcgcgctgaa agtgacatcg ccagcggcat gcctaaccac
     9121 cctaacccga caattgcgac ctttttcatc cgttatctcc tgatacacct tagatctata
     9181 aggctacgct agcgtatcaa aactgacaat tcattctatg aatgaatctg ttcaataatg
     9241 ataacgacat gctgcaatga gcagtgaatc gtcggtaacc gcgtatacca gacggtgctc
     9301 ctctgtaatg cgtcgggacc agaagcctga caaattatgt ttcaggggtt ctggcttccc
     9361 cttaccttca aatggtgttc tgcgtgtatc tttgataatt tcattgatct ttttaacaat
     9421 tcgcttatct gtttcctgcc agtacagata atcatcccat gattcctcag accagattag
     9481 tttcactcaa taatgtcctt ttccgttcct ttgcctgatt tcaggctatc gattgagtcc
     9541 atcaatctcc gggcgttagc gggggaacgc agtagataag ccgtctcttc cagcgagttg
     9601 tattcttcga gtgacatcag aacacaagcc tctccattct gacgagtaat gaggatcggg
     9661 gcatgatctt caacggcttt catcattgtt gccgacaaat tctgacgcgc ttcgctgtag
     9721 ctaattgtac gcatgtaaat ctcctctttt gtacagctca ttgtacaatg atgggcgtta
     9781 attaactatt tattaattag tttgtagatc aagatattgt cagcgagacg aaaaatcaga
     9841 ctttgctctt tttggtgctg tcaagttaga ggacagtcct cttagccccc tcctttcccc
     9901 gctcattcat taaacaaatc catcgccata aaatatataa aaaacccctt gctttctaac
     9961 gtgaaagtgg tttaggttaa aagacatcag ttgaataaac attcacagag atttttatga
    10021 cacgcgttca atttaaacac caccatcatc accatcatcc tgactagtct ttcaggcgat
    10081 gtgtgctgga agacattcag atcttccagt ggtgcatgaa cgcatgagaa agcccccgga
    10141 agatcatctt ccgggggctt ttttattgcg cagttgataa cggttcagac aggtttaaag
    10201 aggaataaga aaatgacaga caacactcgt ttacgcatag ctatgcagaa atccggccgt
    10261 ttaagtgatg actcacgcga attactggcg cgctgtggca ttaaaatcaa tcttcacacc
    10321 cagcgcctga tcgcgatggc agaaaacatg ccgattgata ttctgcgcgt gcgtgacgac
    10381 gacattcccg gtctggtgat ggacggcgtg gtagaccttg ggattatcgg cgaaaacgtg
    10441 ctggaagaag agctgcttaa ccgccgcgcc cagggtgaag atccgcgcta ctttaccctg
    10501 cgtcgtctgg atttcggcgg ctgccgcctt tcgctggcaa cgccggttga tgaagcctgg
    10561 gacggtccgc tctccttaaa cggtaaacgt atcgccacct cttatcctca cctgctcaag
    10621 cgttatctcg accagaaagg tatctctttt aaatcctgct tactgaacgg ttctgtcgaa
    10681 gttgcgccgc gcgccggact ggcggatgcg atttgcgatc tggtttccac cggtgccacg
    10741 ctggaagcta acggcctgcg cgaagtcgaa gttatctacc gctcgaaagc ctgcctgatc
    10801 cagcgcgatg gcgaaatgga agaatccaaa cagcaactga tcgacaaact gctgacccgt
    10861 attcagggtg tgatccaggc gcgcgaatca aaatacatca tgatgcacgc accgaccgaa
    10921 cgcctggatg aagtcatcgc cctgctgcca ggtgccgaac gcccaaccat tctgccgctg
    10981 gcgggtgacc aacagcgcgt agcgatgcac atggtgagta gcgaaaccct gttctgggaa
    11041 acgatggaaa aactgaaagc gttgggtgcc agttcaatcc tggtcctgcc gattgagaag
    11101 atgatggagt gatcgccatg agctttaaca caatcattga ctggaatagc tgtactgcgg
    11161 agcaacaacg ccagctgtta atgcgcccgg cgatctccgc ttctgaaagc attacccgca
    11221 ccgttaacga tattctcgat aacgtgaaaa cgcgtggcga tgaagccctg cgtgaataca
    11281 gcgcgaagtt tgataaaacc acggttaccg cgctgaaggt gtctgctgat gagatcgccg
    11341 ccgccagcga acgcctgagc gacgagctaa aacaggcgat ggcggtggca gtaaagaata
    11401 ttgaaacctt ccacactgcg caaaaactgc cgccggtaga tgtagaaacg cagccaggcg
    11461 tgcgttgcca gcaggttacg cgcccggtgg cttcagtggg tttgtatatt cctggcggct
    11521 ccgccccgct cttctcaacg gtattaatgc tggcaactcc ggcgcgtatt gcgggctgta
    11581 aaaaagtggt gctgtgctca ccgccgccga ttgccgatga gatcctttac gcggcgcagc
    11641 tgtgcggcgt gcaggacgtg tttaacgtcg gcggcgcaca ggccattgcc gcgctgacgt
    11701 ttggtacgga atctgtgccg aaagtggaca aaatcttcgg gccgggtaac gcctttgtca
    11761 ccgaagcaaa acgccaggta agccagcgtc tggacggtgc ggcgatcgat atgcccgcag
    11821 gcccgtcgga agtgctggtg attgctgaca gcggcgctac gccggatttc gtggcttctg
    11881 atttgctttc tcaggctgaa cacggccctg actcacaggt aattttactg acccccgacg
    11941 ctgatatggc gcgtcgcgtt gccgaggccg tcgaacgcca actggcagaa ctgccgcgtg
    12001 ccgaaaccgc ccgccaggca ctgaacgcca gccgcctgat cgtgactaaa gatttagcgc
    12061 agtgcgtaga gatttccaac cagtacggcc cggaacacct gatcattcag acccgcaacg
    12121 cccgcgaact ggtcgatggc atcaccagcg ccggttcggt atttcttggt gactggtcac
    12181 cggaatcggc aggtgactat gcctccggca ctaaccacgt tctgccgact tacggttaca
    12241 ccgccacctg ttccagcctc gggctggcgg atttccagaa acgcatgacc gtgcaggaac
    12301 tgtcgaaaga aggtttttcc gtgctggcgt cgaccattga aacactcgcc gccgccgagc
    12361 gcctgaccgc ccacaaaaat gccgttactt tgcgtgttaa cgcccttaag gagcaagcat
    12421 gagcaccgtg actattaccg atttagcgcg tgaaaacgtc cgcaacctga cgccgtatca
    12481 gtcggcgcgt cgtctgggcg gtaacggcga cgtctggctg aacgccaacg aataccccac
    12541 tgccgtggag tttcagctta ctcagcaaac gctcaaccgc tacccggaat gccagccgaa
    12601 agcggtgatt gaaaattacg cgcaatatgc aggcgtaaaa ccggagcagg tgctggtcag
    12661 ccgtggtgcg gacgaaggta ttgaactgct gattcgcgct ttttgcgaac cgggtaaaga
    12721 cgccatcctc tactgcccgc caacgtacgg catgtacagc gtcagcgccg aaacgattgg
    12781 cgtcgagtgc cgcacagtgc cgacgctgga caactggcaa ctggacttac agggcatttc
    12841 cgacaagctg gacggcgtaa aagtggttta tgtttgcagc cccaataacc cgaccgggca
    12901 actgatcaat ccgcaggatt ttcgcaccct gctggagtta acccgcggta aggcgattgt
    12961 ggttgccgat gaagcctata tcgagttttg cccgcaggca tcgctggctg gctggctggc
    13021 ggaatatccg cacctggcta ttttacgcac actgtcgaaa gcttttgctc tggcggggct
    13081 tcgttgcgga tttacgctgg caaacgaaga agtcatcaac ctgctgatga aagtgatcgc
    13141 cccctacccg ctctcgacgc cggttgccga cattgcggcc caggcgttaa gcccacaggg
    13201 aatcgtcgcc atgcgcgaac gggtagcgca aattattgca gaacgcgaat acctgattgc
    13261 cgcactgaaa gagatcccct gcgtagagca ggttttcgac tctgaaacca actacattct
    13321 ggcgcgcttt aaagcctcca gtgcggtgtt taaatctttg tgggatcagg gcattatctt
    13381 acgtgatcag aataaacaac cctctttaag cggctgcctg cgaattaccg tcggaacccg
    13441 tgaagaaagc cagcgcgtca ttgacgcctt acgtgcggag caagtttgat gagtcagaag
    13501 tatcttttta tcgatcgcga tggaaccctg attagcgaac cgccgagtga ttttcaggtg
    13561 gaccgttttg ataaactcgc ctttgaaccg ggcgtgatcc cggaactgct gaagctgcaa
    13621 aaagcgggct acaagctggt gatgatcact aatcaggatg gtcttggaac acaaagtttc
    13681 ccacaggcgg atttcgatgg cccgcacaac ctgatgatgc agatcttcac ctcgcaaggc
    13741 gtacagtttg atgaagtgct gatttgtccg cacctgcccg ccgatgagtg cgactgccgt
    13801 aagccgaaag taaaactggt ggaacgttat ctggctgagc aagcgatgga tcgcgctaac
    13861 agttatgtga ttggcgatcg cgcgaccgac attcaactgg cggaaaacat gggcattact
    13921 ggtttacgct acgaccgcga aaccctgaac tggccaatga ttggcgagca actcaccaga
    13981 cgtgaccgtt acgctcacgt agtgcgtaat accaaagaga cgcagattga cgttcaggtg
    14041 tggctggatc gtgaaggtgg cagcaagatt aacaccggcg ttggcttctt tgatcatatg
    14101 ctggatcaga tcgctaccca cggcggtttc cgcatggaaa tcaacgtcaa aggcgacctc
    14161 tatatcgacg atcaccacac cgtcgaagat accggcctgg cgctgggcga agcgctaaaa
    14221 atcgccctcg gagacaaacg cggtatttgc cgctttggtt ttgtgctacc gatggacgaa
    14281 tgccttgccc gctgcgcgct ggatatctct ggtcgcccgc acctggaata taaagccgag
    14341 tttacctacc agcgcgtggg cgatctcagc accgaaatga tcgagcactt cttccgttcg
    14401 ctctcataca ccatgggcgt gacgctacac ctgaaaacca aaggtaaaaa cgatcatcac
    14461 cgtgtagaga gtctgttcaa agcctttggt cgcaccctgc gccaggccat ccgcgtggaa
    14521 ggcgataccc tgccctcgtc gaaaggagtg ctgtaatgaa cgtggtgatc cttgataccg
    14581 gctgcgccaa cctgaactcg gtgaagtctg ccattgcgcg tcacggttat gaacccaaag
    14641 tcagccgtga cccggacgtc gtgttgctgg ccgataaact gtttttaccc ggcgttggca
    14701 ctgcgcaagc ggcgatggat caggtacgtg agcgcgagct gtttgatctc atcaaagcct
    14761 gtacccaacc ggtgctgggc atctgcttag ggatgcaact gctggggcgg cgcagcgaag
    14821 agagcaacgg cgtcgacttg ctgggcatca tcgacgaaga cgtgccgaaa atgaccgact
    14881 ttggtctgcc actgccacat atgggctgga atcgcgttta cccgcaggca ggcaaccgcc
    14941 tgtttcaggg gattgaagac ggcgcgtact tttactttgt tcacagctac gcaatgccgg
    15001 tcaatccgtg gaccattgcc cagtgtaatt acggcgaacc gttcaccgcg gcggtacaaa
    15061 aagataactt ctacggcgtg cagttccatc cggagcgttc tggtgccgct ggcgctaagt
    15121 tgctgaaaaa cttcctggag atgtgatgat tattccggca ttagatttaa tcgacggcac
    15181 cgtggtgcgt ctccatcagg gcgattacgg caaacaacgc gattacggta acgacccgct
    15241 gccgcgctta caggattacg ccgcacaagg tgccgaagtg ttgcatctgg tggatctgac
    15301 cggggccaaa gatccggcta agcgtcaaat cccgctgatt aaaaccctgg tcgcgggcgt
    15361 taacgtcccg gtgcaggttg gcggcggtgt gcgtagtgaa gaagacgtgg cggcgttact
    15421 ggaagctggc gttgcgcgcg tggtggtcgg ttccaccgca gtgaaatcac cggacgtagt
    15481 gaaaggctgg tttgaacgtt tcggtgccga tgcgctggtg ctggcactgg atgtccgtat
    15541 tgacgagcaa ggcaacaagc aagtggcagt cagcggctgg caagagaact cgggcgtttc
    15601 actggaacaa ctggtggaaa cctatctgcc cgtcggcctg aaacatgtgt tgtgtaccga
    15661 tatctcgcgc gacggcacgc tggcgggatc taacgtctct ttatatgaag aagtgtgtgc
    15721 cagatatccg caagtggcgt ttcagtcctc cggcggcatt ggcgacatta acgatgttgc
    15781 tgccctacgt ggcactggcg tgcgcggcgt aatagttggt cgtgcattac tggaaggtaa
    15841 attcaccgtg aaggaggcca tcgcatgctg gcaaaacgca taatcccgtg tctcgacgtt
    15901 cgtgatggtc aggtggtgaa aggcgtacag tttcgcaacc atgaaatcat tggcgatatc
    15961 gtgccactgg caaaacgcta cgccgaagaa ggtgcagatg aactggtgtt ctacgatatc
    16021 accgcttcca gcgatggccg tgtggtagat aaaagctggg tatctcgcgt ggcggaagtg
    16081 atcgacattc cgttttgtgt ggcgggtggg attaagtctc tggaagatgc cgcgaaaatt
    16141 ctttcctttg gcgcggataa aatttccatc aactctcctg cgctggcgga cccgacgtta
    16201 attactcgcc tggcggatcg ctttggcgtg cagtgtattg tggtcggtat tgatacctgg
    16261 tacgacgccg aaaccggtaa atatcatgtg aatcaatata ccggcgatga aagccgcacc
    16321 cgcgtcactc aatgggaaac gctcgactgg gtagaggagg tgcaaaaacg cggcgccgga
    16381 gaaatcgtcc tcaacatgat gaatcaggac ggcgtgcgta acggttacga cctcaagcag
    16441 ctgaaaaaag tgcgtgaagt ttgccacgtc ccgctgattg cctccggtgg cgcgggcacc
    16501 atggaacact tcctggaagc cttccgcgat gccgacgttg acggtgcgct ggcagcttcc
    16561 gtattccaca aacaaataat caatattggc gaattaaaag cgtacctggc aacacagggc
    16621 gtggagatca ggatatgtta acagaacaac aacgtcgcga actggactgg gaaaaaaccg
    16681 acggtctgat gccggtgatt gtgcaacacg cggtatccgg cgaagtgtta atgctgggct
    16741 acatgacccc ggaagcctta gacagaacca tcgaaagcgg taaagttacc ttcttctcgc
    16801 gcactaaaca gcgactgtgg accaaaggcg aaacctcggg caatttcctc aatgtggtga
    16861 gcattgcgcc ggactgcgac aacgacacgt tactggtgct ggcgaatccc atcgggccaa
    16921 cctgccacaa aggcaccagc agctgcttcg gcgacaccgc tcaccagtgg ctgttcctgt
    16981 atcaactgga acaactgctc gcggaacgta aatccgccga cccggaaacc tcttacactg
    17041 ccaaactgta tgccagcggc accaaacgca ttgcgcagaa agtgggcgaa gaaggcgtgg
    17101 aaaccgcact ggcagcaacg gtacatgacc gctttgaact gaccaacgag gcgtctgatt
    17161 tgatgtatca cctgctggtg ctgttgcagg atcaggatct ggatttaacg acggtaattg
    17221 agaacctgcg taaacggcat cagtgagttg cgtgctgagc aggtgtgatg ttgttgtcac
    17281 acccggagcc attgcccgat gcgcaagctt atcgtgccta cacattatct caacacacgt
    17341 aggccggata agatgcgcca gcatcgcatc cggcaaaaaa aacgggcatg gtgtcaccac
    17401 cctgcccgtt tctcttaaat gcacaataat attatttcgc gttgtaatta cgcagcgcgt
    17461 tacgcccaag cacaatcccc gcgccaacca tgccgcccag cagcacagcc agaatcaagg
    17521 taattgcttt cttcgggcta tcgcgacgaa taggtaacgt cggtttcatt acatagcggt
    17581 aagcatgaat atcaagttta tcaacgtcaa gattgtcaat atccagcagg ttttgacgag
    17641 tctgatagta gtttgatgag aacaccaacg gacgggtcgc ttcgtgctta atcatcgact
    17701 ccagcgcttc gctccccaaa agaaacatag tatcctgggt tacgtcctgt gtctgttgaa
    17761 tctgcggctt tgtcacctgc gcctgattcg catactgcaa cgcttcctga atctgacgga
    17821 tacgcagatc tttctgctcc tgcgcgacca cttcctgggt tcttaaagag tcctgcaagt
    17881 ttttccgtcc cagagcaatg ttgtccttga gatccttttc tagctcttga ttcactttat
    17941 catcaacttg ctgaatgtat tgggccaact tcatttgtgc gccctctgca gtttgcccaa
    18001 cataagaaac agtcaatggt aattgctggt tcttaacaga aggttcgatg gtaagttttt
    18061 ctggctcttc ctgattatcc agcgtttctg ctaatgcaga gaaggcagaa ctgaagcgac
    18121 caattaacgt ctcctgcaaa tccgatactt tcggtgcagc ctgaccatag ataacattca
    18181 tggcattgtt atagccagca atttgcccca cgtcgggctg agtgataatt gctgttgacg
    18241 tccatttctc cttcgctact gccaaatatc caatagccag ggcaatagcc acaatgacgg
    18301 aaatgatgat tgtcatcttg ccacgccaca actgcactag taaatcaatc aaatcaatct
    18361 gttccgggtc atggttttgc ccagaaacat tattattttc tactctcatc attaccctaa
    18421 ctgacggaaa tttccaagtc cgcatagttt agcgataatc ctcgtgaatg ctataggata
    18481 agtgataaaa ataatgaatt gttgtgtacg taacaaaaga caatcagggc gtaaatagcc
    18541 ctgataacaa gatgttagtc gctgccaaag agatcgcggg tgtatacctt atctgccaca
    18601 tccttaagct cttctgccat tcggttagag ataatgacgt cggcttgttg tttgaaggtg
    18661 gcgagatcac gttccaggcg agagttgaag aatgagtctt ctttcatcac tggctcgtag
    18721 atgatcactt caacaccttt cgccttgata cgtttcataa tcccctgaat agaagacgca
    18781 cggaagttat ctgaaccgct cttcataatc agacgataaa tacccaccac ttgcggcttg
    18841 cgtgacaaaa tggcatcggc aataaaatct ttacgcgtgc ggttagcatc gacaattgcc
    18901 gagatcaggt tattcggcac agactggtag ttcgccagta actgcttggt atctttcggc
    18961 agacaataac caccataacc aaacgacgga ttgttgtaat ggttgccaat acgtgggtcg
    19021 agacaaacgc cttcgattat ttgacgggaa ttcagaccta aactttctgc atagctatcc
    19081 agttcgttaa agtacgccac gcgcatcgcc aggtaggtgt ttgcaaaaag tttaatcgct
    19141 tctgcttcag tggagtcggt aaacagcatc gggatatttt gcttaatcgc gccttcctgt
    19201 aacagagcag cgaaacgttc tgcgcgttct gaacgctcac cgatgacaat acgtgaagga
    19261 tggagattat cgtaaagggc tttaccctca cggagaaatt ccggggagaa tataatattt
    19321 tcagtgcgat atttcttatg catcgctgcg gtaaaaccaa cgggaaccgt tgatttgatg
    19381 accataaccg cataaggatt tatctcaact acgtctttaa ttactgattc tacactggat
    19441 gtattgaaat aattagtttt aggatcatag tcggttggag tggcgatgat gacataatca
    19501 gcatcccggt aggcttcatt tttatctaat gtggcattaa agtgtatttt atctgattgc
    19561 aaaaactgct gaatttcctt atcaacaata ggagatatcc gatcattcag catagcaacg
    19621 cgtgacggta aaatatctaa tgccacaacc tcatgatttt gtgcgattag aagcccgttt
    19681 gacaagccta catagccagt accggaaatg gtgattttca tttcatgctc tcagaattaa
    19741 cttaactgtg aatcatgatg tttttagcat cctgataaga gctaaaagtt ttaacgctac
    19801 gttgttcgat ggtcaacgca acgaa
//
LOCUS       DAHHTG010000079        19784 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.079, whole genome shotgun sequence.
ACCESSION   DAHHTG010000079 DAHHTG010000000
VERSION     DAHHTG010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19784)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19784)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19784
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.079"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            184..2574
                     /gene="gcd"
                     /locus_tag="NBI74_002940"
     CDS             184..2574
                     /gene="gcd"
                     /locus_tag="NBI74_002940"
                     /EC_number="1.1.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinoprotein glucose dehydrogenase"
                     /protein_id="HCD1933234.1"
                     /translation="MAINNTGSRRLLVTLTALFAALCGLYLLIGGGWLVAIGGSWYYP
                     IAGLVMLGVAWMLWRSKRAALWLYAALLLGTMIWGVWEVGFDFWALTPRSDILVFFGI
                     WLILPFVWRRLVIPASGAVAALVVALLISGGILTWAGFNDPQEISGTLSADATPAEAI
                     SPVADQDWPAYGRNQEGQRFSPLKQINADNVHKLKEAWVFRTGDVKQPNDPGEITNEV
                     TPIKVGDTLYLCTAHQRLFALDAASGKEKWHYDPELKTNESFQHVTCRGVSYHEAKAE
                     TASPEVMADCPRRIILPVNDGRLIAINAENGKLCETFANKGVLNLQSNMPDTKPGLYE
                     PTSPPIITDKTIVMAGSVTDNFSTRETSGVIRGFDVNTGELLWAFDPGAKDPNAIPSD
                     EHTFTFNSPNSWAPAAYDAKLDLVYLPMGVTTPDIWGGNRTPEQERYASSILALNATT
                     GKLAWSYQTVHHDLWDMDLPAQPTLADITVNGQKVPVIYAPAKTGNIFVLDRRNGELV
                     VPAPEKPVPQGAAKGDYVTPTQPFSELSFRPTKDLSGADMWGATMFDQLVCRVMFHQM
                     RYEGIFTPPSEQGTLVFPGNLGMFEWGGISVDPNREVAIANPMALPFVSKLIPRGPGN
                     PMEQPKDAKGTGTESGIQPQYGVPYGVTLNPFLSPFGLPCKQPAWGYISALDLKTNEV
                     VWKKRIGTPQDSMPFPMPVPVPFNMGMPMLGGPISTAGNVLFIAATADNYLRAYNMSN
                     GEKLWQGRLPAGGQATPMTYEVNGKQYVVISAGGHGSFGTKMGDYIVAYALPDDVK"
     gene            complement(2621..4171)
                     /gene="cueO"
                     /locus_tag="NBI74_002941"
     CDS             complement(2621..4171)
                     /gene="cueO"
                     /locus_tag="NBI74_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase CueO"
                     /protein_id="HCD1933235.1"
                     /translation="MQRRDFLKYSVALGVASALPLWSRAVFAAERPTLPIPDLLTTDA
                     RNRIQLTIGAGQSTFGGKTATTWGYNGNLLGPAVKLQRGKAVTVDIYNQLTEETTLHW
                     HGLELPGEVDGGPQGIIPPGGKRSVTLNVDQPAATCWFHPHQHGKTGRQVAMGLAGLV
                     VIEDDEILKLMLPKQWGIDDVPVIVQDKKFNADGQIDYQLDVMTAAVGWFGDTLLTNG
                     AIYPQHAAPRGWLRLRLLNGCNARSLNFATSDNRPLYVIASDGGLLPEPVKVNELPVL
                     MGERFEVLVEVNDNKPFDLVTLPVSQMGMAITPFDKPHPVMRIQPIAISASGALPDTL
                     SSLPALPSLEGLTVRKLQLSMDPMLDMMGMQMLMEKYGDQAMAGMDHSQMMGHIGHGN
                     MNHMNHSGKFDFHHANKINGQAFDMNKPMFAAAKGQYERWVISGVGDMMLHPFHIHGT
                     QFRILSENGKPPATHRAGWKDTVKVEGNVSEVLVKFNHDAPKEHAYMAHCHLLEHEDT
                     GMMLGFTV"
     gene            4337..4684
                     /locus_tag="NBI74_002942"
     CDS             4337..4684
                     /locus_tag="NBI74_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacC family pilotin-like protein"
                     /protein_id="HCD1933236.1"
                     /translation="MKTFFRTVLFGSLMAVCANSYALSESEAEDMADLTAVFVFLKND
                     CGYQNLPNGQIRRALVFFAQQNQWDLSNYDTFDMKALGEDSYRDLSGIGIPVAKKCKA
                     LARDSLSLLAYVK"
     gene            4790..5656
                     /gene="speE"
                     /locus_tag="NBI74_002943"
     CDS             4790..5656
                     /gene="speE"
                     /locus_tag="NBI74_002943"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HCD1933237.1"
                     /translation="MAEKKQWHETLHDQFGQYFAVDNVLYHEKTDHQDLIIFENAAFG
                     RVMALDGVVQTTERDEFIYHEMMTHVPLLAHGHAKHVLIIGGGDGAMLREVTRHKNVE
                     SITMVEIDAGVVSFCRQYLPNHNAGSYDDPRFKLVIDDGVNFVNQTSQTFDVIISDCT
                     DPIGPGESLFTSAFYEGCKRCLNPGGIFVAQNGVCFLQQEEAIDSHRKLSHYFSDVGF
                     YQAAIPTYYGGIMTFAWATDNDALRHLSTEIIQARFLASGLKCRYYNPAVHTAAFALP
                     QYLQDALASQPS"
     gene            5672..6466
                     /gene="speD"
                     /locus_tag="NBI74_002944"
     CDS             5672..6466
                     /gene="speD"
                     /locus_tag="NBI74_002944"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HCD1933238.1"
                     /translation="MKKLKLHGFNNLTKSLSFCIYDICYAKTTEERDGYIAYIDELYN
                     ANRLTEILSETCSIIGANILNIARQDYEPQGASVTILVSEEPVDPKLIDKTEHPGPLP
                     ETVVAHLDKSHICVHTYPESHPEGGLCTFRADIEVSTCGVISPLKALNYLIHQLESDI
                     VTIDYRVRGFTRDINGMKHFIDHEINSIQNFMSEDMKALYDMVDVNVYQENIFHTKML
                     LKEFDLKHYMFHTKPEDLTDSERQEITAALWKEMREIYYGRNMPAV"
     gene            complement(6504..6866)
                     /locus_tag="NBI74_002945"
     CDS             complement(6504..6866)
                     /locus_tag="NBI74_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacL family protein"
                     /protein_id="HCD1933239.1"
                     /translation="MDYEFLRDITGVVKVRMSMGHEVVGHWFNEEVKENLALLDEVEQ
                     AAHALKGSERSWQRAGHEYTLWMDGEEVMVRANQLEFAGDEMEEGMNYYDEESLSLCG
                     VEDFLQVVAAYRNFVQQK"
     gene            complement(7041..9638)
                     /gene="acnB"
                     /locus_tag="NBI74_002946"
     CDS             complement(7041..9638)
                     /gene="acnB"
                     /locus_tag="NBI74_002946"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019080489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aconitate hydratase
                     2/2-methylisocitrate dehydratase"
                     /protein_id="HCD1933240.1"
                     /translation="MLEEYRKHVAERAAEGIAPKPLDANQMAALVELLKNPPAGEEEF
                     LLDLLTNRVPPGVDEAAYVKAGFLAAVAKGEAKSPLLTPEKAIELLGTMQGGYNIHPL
                     IDALDDAKLAPIAAKALSHTLLMFDNFYDVEEKAKAGNEYAKQVMQSWADAEWFLNRP
                     ALAEKLTVTVFKVTGETNTDDLSPAPDAWSRPDIPLHALAMLKNAREGIEPDQPGVVG
                     PIKQIEALQQKGFPLAYVGDVVGTGSSRKSATNSVLWFMGDDIPHVPNKRGGGLCLGG
                     KIAPIFFNTMEDAGALPIEVDVSNLNMGDVIDVYPYKGEVRNHETGELLATFELKTDV
                     LIDEVRAGGRIPLIIGRGLTTKAREALGLPHSDVFRQAKDVAESDRGFSLAQKMVGRA
                     CGVKGIRPGAYCEPKMTSVGSQDTTGPMTRDELKDLACLGFSADLVMQSFCHTAAYPK
                     PVDVNTHHTLPDFIMNRGGVSLRPGDGVIHSWLNRMLLPDTVGTGGDSHTRFPIGISF
                     PAGSGLVAFAAATGVMPLDMPESVLVRFKGKMQPGITLRDLVHAIPLYAIKQGLLTVE
                     KKGKKNIFSGRILEIEGLPDLKVEQAFELTDASAERSAAGCTIKLNKEPIIEYLNSNI
                     VLLKWMIAEGYGDRRTLERRIQGMEKWLANPELLEADADAEYAAVIDIDLADIKEPIL
                     CAPNDPDDARPLSAVQGEKIDEVFIGSCMTNIGHFRAAGKLLDAHKGQLPTRLWVAPP
                     TRMDAAQLTEEGYYSVFGKSGARIEIPGCSLCMGNQARVADGATVVSTSTRNFPNRLG
                     TGANVFLASAELAAVAALIGKLPTPEEYQTYVAQVDKTAVDTYRYLNFDQLSQYTEKA
                     DGVIFQTAV"
     gene            9993..11756
                     /locus_tag="NBI74_002947"
     CDS             9993..11756
                     /locus_tag="NBI74_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3300 domain-containing protein"
                     /protein_id="HCD1933241.1"
                     /translation="MKMTLPFKPHVLALICSAGLCAASAGLYIKSRTVEAPVEAQSTQ
                     QTAPDISAVTLPATVSVPPVTPAVVKSAFSTAQIDQWVAPVALYPDSLLSQVLMASTY
                     PANVAQAVQWSHDNPLKQGDAAIQAVSDQPWDASVKSLVAFPQLMALMGENPQWVQNL
                     GDAFLAQPQDVMDSVQRLRQLAQQTGSLKSSTEQKIITTTKKVVPVNQPANAPATQSN
                     TVSTSSPVVAEPAPTVITIEPANPDVVYIPNYNPNVVYGSWANTAYPPVYLPPPAGEP
                     FVDSFVRGFGYSMGVATTYALFSSIDWDDDDHDHDHHHHDDDDYHHHDGGHRDGNDWQ
                     HNGDNINIDVNNFNRITGEHLTDKNMAWRHNPNYRNGVTYHDQDMAKRFHQTDVNGGM
                     SATQLPALSRDSQRQAAASQFQQRTHAAPVITRDTQRQAAAQRFNEAEHYGNYDDFRE
                     FSRHQPLTQQQKDAARQRYQSASPEQRQAAREKMQTSPQNQQQRDAARQRIQSASPEQ
                     RQVFKEKVQQRPLNQEQRDNARQRVQSASPEQRPFFREKVQESRPQRLNDSNRTARLN
                     NEQRSAVRERLSERGARRLER"
     gene            complement(11827..13251)
                     /gene="lpdA"
                     /locus_tag="NBI74_002948"
     CDS             complement(11827..13251)
                     /gene="lpdA"
                     /locus_tag="NBI74_002948"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HCD1933242.1"
                     /translation="MSTEIKTQVVVLGAGPAGYSAAFRCADLGLETVIVERYNTLGGV
                     CLNVGCIPSKALLHVAKVIEEAKALAEHGIVFGEPKTDIDKIRTWKEKVINQLTGGLA
                     GMAKGRKVKVVNGLGKFTGANTLEVEGENGKTVINFDNAIIAAGSRPIQLPFIPHEDP
                     RIWDSTDALELKEVPERLLVMGGGIIGLEMGTVYHALGSQIDVVEMFDQVIPAADKDI
                     VKVFTKRISKKFNLMLETKVTAVEAKEDGIYVTMEGKKAPAEPQRYDAVLVAIGRVPN
                     GKNLDAGKAGVEVDDRGFIRVDKQLRTNVPHIFAIGDIVGQPMLAHKGVHEGHVAAEV
                     IAGKKHYFDPKVIPSIAYTEPEVAWVGLTEKEAKEKGISYETATFPWAASGRAIASDC
                     ADGMTKLIFDKESHRVIGGAIVGTNGGELLGEIGLAIEMGCDAEDIALTIHAHPTLHE
                     SVGLAAEVFEGSITDLPNPKAKKK"
     gene            complement(13459..15351)
                     /gene="aceF"
                     /locus_tag="NBI74_002949"
     CDS             complement(13459..15351)
                     /gene="aceF"
                     /locus_tag="NBI74_002949"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex
                     dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HCD1933243.1"
                     /translation="MAIEIKVPDIGADEVEITEILVKVGDKVEAEQSLITVEGDKASM
                     EVPSPQAGIVKEIKVSVGDKTQTGALIMIFDSADGAADAAPAQAEEKKEAAPAAAPAA
                     AAAKDVNVPDIGSDEVEVTEILVKVGDKVEAEQSLITVEGDKASMEVPAPFAGTVKEI
                     KVNVGDKVSTGSLIMVFEVAGEAGAAAPAAKQEAAPAAASAPAAGVKEVNVPDIGGDE
                     VEVTEVMVKVGDKVAAEQSLITVEGDKASMEVPAPFAGVVKELKVNVGDKVKTGSLIM
                     IFEVEGAAPAAAPAKQEAAAPAPAAKAEAPAAAPAAKAEGKSEFAENDAYVHATPLIR
                     RLAREFGVNLAKVKGTGRKGRILREDVQAYVKEAIKRAEAAPAATGGGIPGMLPWPKV
                     DFSKFGEIEEVELGRIQKISGANLSRNWVMIPHVTHFDKTDITELEAFRKQQNEEAAK
                     RKLDVKITPVVFIMKAVAAALEQMPRFNSSLSEDGQRLTLKKYINIGVAVDTPNGLVV
                     PVFKDVNKKGIIELSRELMTISKKARDGKLTAGEMQGGCFTISSIGGLGTTHFAPIVN
                     APEVAILGVSKSAMEPVWNGKEFVPRLMLPISLSFDHRVIDGADGARFITIINNTLSD
                     IRRLVM"
     gene            complement(15366..18029)
                     /gene="aceE"
                     /locus_tag="NBI74_002950"
     CDS             complement(15366..18029)
                     /gene="aceE"
                     /locus_tag="NBI74_002950"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008814576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HCD1933244.1"
                     /translation="MSERFPNDVDPIETRDWLQAIESVIREEGVERAQYLIDQLLAEA
                     RKGGVNVAAGTGISNYINTIPVEEQPEYPGNLELERRIRSAIRWNAIMTVLRASKKDL
                     ELGGHMASFQSSATIYDVCFNHFFRARNEQDGGDLVYFQGHISPGVYARAFLEGRLTQ
                     EQLDNFRQEVHGNGLSSYPHPKLMPEFWQFPTVSMGLGPIGAIYQAKFLKYLEHRGLK
                     DTSKQTVYAFLGDGEMDEPESKGAITIATREKLDNLVFVINCNLQRLDGPVTGNGKII
                     NELEGIFEGAGWNVIKVMWGSRWDELLRKDTSGKLIQLMNETVDGDYQTFKSKDGAYV
                     REHFFGKYPETAALVADWTDEQIWALNRGGHDPKKIYAAFKKAQETKGKATVILAHTI
                     KGYGMGDAAEGKNIAHQVKKMNMDGVRHIRDRFNVPVSDADIEKLPYITFPEGSEEHT
                     YLHAQRQKLHGYLPSRQPNFTEKLELPSLQDFGALLEEQSKEISTTIAFVRALNVMLK
                     NKSIKDRLVPIIADEARTFGMEGLFRQIGIYSPNGQQYTPQDREQVAYYKEDEKGQIL
                     QEGINELGAGCSWLAAATSYSTNNLPMIPFYIYYSMFGFQRIGDLCWAAGDQQARGFL
                     IGGTSGRTTLNGEGLQHEDGHSHIQSLTIPNCISYDPAYAYEVAVIMHDGLERMYGEK
                     QENVYYYITTLNENYHMPAMPEGAEEGIRKGIYKLETIEGSKGKVQLLGSGSILRHVR
                     EAAEILAKDYGVGSDVYSVTSFTELARDGQDCERWNMLHPLETPRVPYIAQVMNDAPA
                     VASTDYMKLFAEQVRTYVPADDYRVLGTDGFGRSDSRENLRHHFEVDASYVVVAALGE
                     LAKRGEIDKKVVADAIAKFNIDADKVNPRLA"
     gene            complement(18190..18954)
                     /gene="pdhR"
                     /locus_tag="NBI74_002951"
     CDS             complement(18190..18954)
                     /gene="pdhR"
                     /locus_tag="NBI74_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex transcriptional
                     repressor PdhR"
                     /protein_id="HCD1933245.1"
                     /translation="MAYSKIRQPKLSDVIEQQLEFLILEGTLRPGEKLPPERELAKQF
                     DVSRPSLREAIQRLEAKGLLLRRQGGGTFVQSSLWQSFSDPLVELLSDHPESQYDLLE
                     TRHALEGIAAYYAALRSTDEDKERIRELHHAIELAQQSGDLDAESNAVLQYQIAVTEA
                     AHNVVLLHLLRCMEPMLAQNVRQNFELLYSRREMLPLVSSHRTRIFEAIMAGKPEEAR
                     EASHRHLAFIEEILLDRSREESRRERSLRRLEQRKN"
     gene            complement(19410..19700)
                     /locus_tag="NBI74_002952"
     CDS             complement(19410..19700)
                     /locus_tag="NBI74_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000282231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HCD1933246.1"
                     /translation="MYNFKDKIEDYTEREFIELLGEFTNPTGDNAQLKGEELDKYWDD
                     LEEHLTRITQHPLMSDLIYYPAKKGDDEPENILKIVKEWRRSQGLPLFKDSE"
BASE COUNT         4607 a         5411 c         4904 g         4862 t
ORIGIN      
        1 aacaaatcac ttcgcacggg caggttttgg caccgcaaca aagcgcaaga cacgcagtat
       61 accagtaaaa ctattcattc gcggaatctg ttaataaagc tcgaattgtg atgacgatca
      121 cacatgttaa aacctataat taattgctac taaaatatta atgaattgaa atggtgtctc
      181 tttatggcaa ttaacaatac cggctcgcgg cgattactcg tcacgctaac agcccttttt
      241 gcagcgcttt gcgggctgta tctactcatt ggcggaggct ggctggtcgc gattggcggc
      301 tcctggtact accctatcgc tggccttgtg atgctcggcg tcgcctggat gctgtggcgc
      361 agtaaacgcg ccgcgctttg gctgtacgca gccctgctgc tcggcactat gatttggggc
      421 gtctgggaag ttggtttcga cttctgggcg ctgactccgc gcagcgacat tctggtcttc
      481 ttcggcatct ggttgatcct gccgtttgtc tggcgtcgcc tggtcattcc tgccagcggc
      541 gcagttgccg cactggtggt ggcgctgttg attagcggtg gtatcctgac ctgggccgga
      601 tttaacgatc cgcaggaaat cagcggcacc ttaagcgccg atgccacacc tgccgaagcc
      661 atctcccccg ttgccgatca ggactggcct gcctatggtc gcaatcagga aggccaacgc
      721 ttttcaccac tgaaacaaat taacgctgat aacgtccaca agctgaaaga agcctgggtg
      781 ttccgtactg gcgatgtgaa gcagccgaac gatccaggtg aaatcaccaa tgaagtgacg
      841 ccaattaaag tgggcgacac cctttacctg tgtaccgcgc accagcgcct gtttgcgctg
      901 gatgccgcca gcggcaaaga gaaatggcat tacgatcctg agctgaaaac caacgagtcc
      961 ttccagcatg taacctgtcg tggcgtctct tatcatgaag ccaaagcaga aactgcctcg
     1021 ccagaagtga tggcggattg cccgcgtcgt atcattcttc cggtcaatga tggtcgtctg
     1081 attgcaatta acgctgaaaa cggcaagctg tgcgaaacct tcgccaataa aggcgtgctc
     1141 aatctgcaaa gcaatatgcc ggacaccaaa ccgggtctgt atgaaccgac ttcgccgccg
     1201 attatcaccg ataaaaccat cgtgatggcc ggttcagtca ccgacaactt ctcaacccgc
     1261 gaaacgtctg gcgtgatccg tggttttgat gtcaacaccg gggagctgct gtgggctttt
     1321 gatccgggcg cgaaagaccc gaatgcgatc ccgtctgacg aacacacctt tacctttaac
     1381 tcaccaaact cgtgggcacc agcggcctat gacgcgaagc tggatctggt ctatctgccg
     1441 atgggcgtca ccacgccaga tatctggggc ggtaatcgca caccggaaca ggaacgttat
     1501 gccagctcga ttctggcgct gaatgccact accgggaaac tggcgtggag ctaccagacc
     1561 gttcaccacg acctgtggga tatggatctt ccggcacagc cgacgctggc ggacatcacc
     1621 gttaatggtc agaaagtgcc ggttatttac gctccggcga aaaccggcaa catttttgtg
     1681 ctcgatcgtc gtaatggtga actggtggtt ccggccccgg aaaaaccggt tccccaaggt
     1741 gctgccaaag gcgattacgt tacccctact caacccttct cggaactgag cttccgtccg
     1801 acgaaagatt tgagcggtgc ggatatgtgg ggtgccacca tgtttgacca gttggtgtgc
     1861 cgcgtgatgt tccaccagat gcgctatgaa ggcattttta cgccgccatc tgaacagggt
     1921 acactggtct tcccgggtaa tctggggatg ttcgaatggg gcgggatttc cgttgatccg
     1981 aatcgtgaag tggcgattgc caacccaatg gcactgccgt ttgtttcgaa actgattcca
     2041 cgcggtcctg gcaacccgat ggaacagccg aaagatgcca aaggcacggg tacggaatcc
     2101 ggcatccagc cgcagtacgg tgtaccgtat ggcgtcacgc tcaacccgtt cctctcaccg
     2161 tttggtctgc catgtaaaca gccagcatgg ggctatatct cggcattgga tctgaaaacc
     2221 aatgaagtgg tgtggaagaa acgtattggt acgccgcagg acagtatgcc attcccgatg
     2281 cctgtaccgg tgccgttcaa tatgggtatg ccgatgcttg gcgggccaat ctccacggcg
     2341 ggtaacgtgc tgtttatcgc cgctacggca gataactacc tgcgcgctta caacatgagc
     2401 aacggtgaaa aactgtggca gggtcgttta ccagcgggtg gtcaggctac gccaatgacc
     2461 tatgaagtga atggcaagca gtatgtggtg atctccgcag gcggtcacgg ttcatttggt
     2521 acgaagatgg gcgactatat tgtggcttat gcgctgccgg atgatgtgaa gtaaccataa
     2581 tgcccggaga gatccgggca tatttccgaa tacggtcttt ttataccgta aaccctaaca
     2641 tcatccccgt atcttcatgc tccagcagat ggcagtgcgc catataagca tgttctttcg
     2701 gtgcatcgtg attaaacttc accagcactt cgctgacatt accttctacc ttaacggtat
     2761 ctttccagcc tgcgcgatgc gtcgctggcg gtttgccatt ttctgacaag atacggaact
     2821 gcgtaccgtg gatatggaac ggatgcagca tcatgtcgcc cacgccagag ataacccaac
     2881 gttcgtactg ccctttcgcc gccgcaaaca tcggcttatt catatcaaac gcctgaccgt
     2941 tgattttgtt ggcatggtgg aaatcgaact tcccgctgtg gttcatatga ttcatattgc
     3001 cgtgccctat atggcccatc atctggctgt gatccatccc ggccatggcc tgatcgccat
     3061 atttctccat cagcatctgc atccccatca tatcgagcat tgggtccata gaaagttgca
     3121 gcttgcgtac cgtcagccct tccagcgaag gtaacgcagg caggctactt aatgtgtctg
     3181 gcaaagcacc agaggcacta atggcaatcg gctgaatccg cattaccgga tgcggcttgt
     3241 caaacggcgt aatcgccatc cccatctggc tgaccggcag cgtcaccagg tcaaagggtt
     3301 tgttgtcgtt aacctccacc agcacttcaa aacgctcgcc catcagcact ggcagctcgt
     3361 tcaccttcac cggttcaggt agcagaccgc cgtcgctggc aatcacataa agcgggcgat
     3421 tgtcgctggt ggcgaaattg agcgagcggg cattacagcc attgagcaaa cgcaggcgca
     3481 gccagccacg cggggcagcg tgttgcgggt agattgcacc gttggtcagc aacgtatcgc
     3541 caaaccaacc cacagcggcg gtcatcacat ccagttgata atcaatctgc ccgtctgcgt
     3601 taaatttttt atcctgaaca atcaccggga catcatcaat accccactgt tttggcagca
     3661 ttaatttcag gatctcgtca tcttcaatca ccaccaaacc agccagcccc atcgccacct
     3721 gacgtccggt cttgccatgt tgatgtggat ggaaccagca ggtagcggca ggttgatcaa
     3781 cgttcaacgt caccgagcgc ttgccacctg gcggaataat tccctgcggg ccgccgtcca
     3841 cttcacccgg tagttccagc ccgtgccagt gcaacgtcgt ctcttccgtc agttggttgt
     3901 agatatcaac cgttaccgct ttgccgcgct gtaatttcac cgccggcccc agcagattgc
     3961 cgttatagcc ccaggtagtt gcggttttcc cgccaaaggt ggactgacct gcgccaatag
     4021 ttaactgaat gcgattacgg gcatcggtcg tgagcaaatc agggattggt aacgttgggc
     4081 gttccgccgc aaatactgcg cggctccaca gcggcaaggc tgaagccaca cccagcgcga
     4141 cggaatattt caagaaatca cgacgttgca tagttatttc cttattctta agcaggcgaa
     4201 acaaaatcaa acgttgagca tagtccttcc ccttacggga aggtcaagcc gcagacacaa
     4261 tttaatcgac aataatggtc gtcgcctcgc tcgcagtgtg ctaacgttta tcttctttaa
     4321 gcccctggta gaagccatga agacgttttt cagaacagtg ttattcggca gcctgatggc
     4381 cgtttgcgca aacagttacg cgctcagcga gtctgaagcc gaagatatgg ccgatttaac
     4441 ggcagttttt gtttttctga agaacgattg tggttaccag aacttaccta acgggcaaat
     4501 tcgtcgcgcg ctggtctttt tcgcccagca aaaccagtgg gatctcagta attacgacac
     4561 cttcgacatg aaagccctcg gtgaagacag ctaccgcgat ctcagtggca ttggcattcc
     4621 cgtcgctaaa aagtgcaaag ccctggcccg cgattcctta agcctgcttg cctacgtcaa
     4681 ataatccctg ataccttttt gtagaaatat tgaccgtgca tccgcggtca atgttagcta
     4741 ttatgttgcg ccattttttt acgggtgtta acaaaggagg tatcaaccca tggccgaaaa
     4801 aaaacagtgg catgaaacgc tacacgacca gtttgggcag tactttgcgg tagataacgt
     4861 tctgtatcat gaaaagaccg atcaccagga tctgatcatt tttgagaacg ccgcatttgg
     4921 tcgcgtaatg gcgctggatg gcgtagtaca aaccaccgag cgcgacgagt ttatctatca
     4981 tgagatgatg acccatgttc cgctactggc ccacggtcac gcgaaacatg tgctgattat
     5041 cggcggcggc gacggtgcca tgctgcgtga agtgactcga cataaaaacg ttgagtcaat
     5101 cacgatggtg gaaatcgatg cgggtgttgt gtcgttctgc cgtcagtatc ttcccaacca
     5161 taacgccggt agttacgacg atccacgctt taaactggtg atcgacgatg gtgtcaattt
     5221 cgttaatcaa accagccaga cctttgatgt cattatctcc gactgcaccg atcctatcgg
     5281 tcccggcgaa agtctgttca cttcggcatt ttatgaaggc tgcaaacgtt gcctgaatcc
     5341 tggcggtatt ttcgttgcgc agaacggcgt ctgcttttta cagcaggaag aggccatcga
     5401 cagtcatcgc aaactcagcc attacttcag cgatgttggc ttttatcagg cggcgatccc
     5461 gacctattac ggcggcatca tgacctttgc atgggcgaca gataacgacg ccttacgcca
     5521 tctctcaacc gaaattattc aggcgcgttt tctcgcttct ggcctgaaat gccgttatta
     5581 caatccggca gtccatacgg cagcttttgc cttaccccag tatctgcaag atgcactggc
     5641 ttcacagccg tcctaaggag aagataagaa attgaaaaaa ctgaaactgc atggctttaa
     5701 taatctgacc aaaagtctga gtttttgtat ttacgatatc tgctacgcca aaactaccga
     5761 agagcgcgac ggttatattg cttatatcga tgaactctat aatgccaacc gtctgaccga
     5821 aatcctgtca gaaacctgtt ccattatcgg ggccaatatt cttaacatcg cccgccagga
     5881 ttacgaacca caaggtgcca gcgtcactat tctggtgagt gaagaaccgg tcgacccgaa
     5941 acttatcgac aaaacagaac accccggccc actgccagaa acggtcgttg cccatctcga
     6001 taaaagtcat atttgcgtac atacctaccc ggaaagtcat cctgaaggcg gtttatgtac
     6061 cttccgcgcc gatattgaag tctctacctg cggcgtgatt tctccgctga aggcgctgaa
     6121 ttacctgatc caccagcttg agtccgatat tgtgactatt gattatcgcg tgcgcggttt
     6181 tacccgcgac attaacggta tgaagcactt tatcgaccat gagattaatt cgattcagaa
     6241 ctttatgtcc gaagatatga aggcgctgta tgacatggtg gatgtgaacg tctatcagga
     6301 gaatatcttc cataccaaga tgttgcttaa agagttcgac cttaagcact acatgttcca
     6361 caccaaaccg gaagatttaa ccgacagcga gcgccaggaa attaccgctg cgctgtggaa
     6421 agaaatgcgc gagatttatt acgggcgcaa tatgcccgct gtttaacgtc tcaggcggag
     6481 cacggaggct ccgcctgatt tacttacttc tgctgcacga aattacggta agccgccacc
     6541 acctgaagaa aatcctcaac gccgcacagc gacaagctct cttcatcgta gtagttcatc
     6601 ccctcttcca tttcatcgcc agcgaattcc agttgattgg cgcgaaccat cacctcttca
     6661 ccgtccatcc acagagtgta ttcatgccct gcccgttgcc aggatcgttc actacctttc
     6721 aatgcgtgcg ccgcttgttc cacttcatca agcaaggcca ggttttcttt cacctcttca
     6781 ttaaaccagt gcccgaccac ttcatgcccc atggacatgc gcacctttac cactccggta
     6841 atatcgcgca gaaattcgta atccatagtg tgttcctctg ctcccggcaa tgcgccgaaa
     6901 tcgcattgcg ccattgatgt aattatcgca gccttaccgg agaaaaacag cggaacggcg
     6961 atggtttaga aataaaaaat gcccgggaat ctcattcacc gggcattgtg tcgtttatgc
     7021 gcagcgcgtg cgcttactta ttaaaccgca gtctggaaaa tcaccccgtc cgctttttcg
     7081 gtgtactgag aaagctggtc gaagttcaga taacggtaag tatcaacggc tgttttatct
     7141 acctgcgcca cgtaggtctg gtactcttcc ggcgtcggca gtttgccaat cagcgccgca
     7201 acagcagcca gttccgcaga agccaggaag acattcgcgc cagtacccaa acggttcggg
     7261 aagttacggg tagaggtgga aaccaccgtc gcaccgtctg ctacacgcgc ctggttaccc
     7321 atacacaggg aacagccagg gatctcgata cgagcaccac tcttaccgaa gacgctgtag
     7381 tagccttctt cggtcagctg cgcagcgtcc atacgcgttg gcggtgccac ccacaggcgg
     7441 gtcggcaact gacctttgtg cgcatccagc agtttaccag cagcacggaa gtgaccgatg
     7501 ttagtcatgc aagaaccgat aaatacttcg tcgatcttct cgccctgtac cgcagacagc
     7561 ggacgtgcgt cgtccgggtc attcggtgca cagaggattg gctctttgat atccgccaga
     7621 tcgatgtcga tcactgccgc gtattccgca tctgcatcgg cttccagcag ctcaggattc
     7681 gccagccatt tttccatgcc ctgaatacga cgctccaggg tacgacgatc gccgtaacct
     7741 tccgcgatca tccacttcag caggacgatg ttagagttca ggtattcaat gatcggttct
     7801 ttgttcagct tgatggtaca accggcagca gaacgctcgg cagatgcatc ggtcagctca
     7861 aatgcctgct caactttcag atccggcaga ccttcaattt ccagaatacg gccagagaag
     7921 atgtttttct tacctttctt ctcaacggtc agcagacctt gtttgattgc atacagcgga
     7981 atggcgtgta ccagatcgcg cagggtgata cccggctgca ttttgccttt gaagcgtacc
     8041 agaacggatt ccggcatatc cagcggcatt acgcctgttg cggctgcaaa tgccaccaga
     8101 ccagaacccg ccgggaaaga gataccgatc gggaaacggg tatgggagtc accaccggta
     8161 ccgacggtat ccggcagcag catacggttc agccaggagt gaatgacgcc gtcccccgga
     8221 cgcagcgaca caccgccacg gttcataatg aagtccggca gcgtgtggtg cgtgttcacg
     8281 tcaactggct tcggatacgc cgcggtgtgg cagaaagact gcatcaccag gtcagccgag
     8341 aagcccaggc acgccaggtc tttcagttca tcacgggtca tcgggccggt ggtgtcctgg
     8401 gaacctacag aagtcatttt cggttcacag tacgcacccg gacgaatgcc tttcacgcca
     8461 caggcacggc ctaccatttt ctgcgccagc gagaagccgc gatcgctctc agcgacatct
     8521 ttcgcctgac ggaacacatc actatgcggc aggccaagtg cttcacgcgc tttggtggtc
     8581 aggccacgcc cgataatcag cggaatacgg ccgccagcac gcacttcatc aatcagcacg
     8641 tcggttttca gttcgaaggt tgccagcagt tcgccggttt cgtggttacg cacttcacct
     8701 ttgtacgggt aaacgtcaat tacgtcgccc atgttcaggt tagagacgtc gacttcgatt
     8761 ggcagtgcac ccgcgtcttc catcgtgtta aagaagattg gtgcaatttt accgccgagg
     8821 cacaaaccac cgccgcgttt gttcggcaca tgtggaatat catcgcccat aaaccacaga
     8881 acggagttcg tggcggattt acgcgatgac cccgtaccca caacgtcacc gacgtacgcc
     8941 agcgggaaac ctttctgttg cagagcttca atctgtttga tcgggccaac aacaccaggc
     9001 tgatctggct caataccttc acgggcgttt ttcagcatcg ccagcgcgtg cagtgggata
     9061 tccgggcgtg accacgcatc cggtgccgga gaaaggtcat cggtgttggt ttcgccggtc
     9121 actttgaaga cggtaacggt cagtttttca gccagcgccg ggcgattcag gaaccattcg
     9181 gcatccgccc aggactgcat tacctgcttc gcatattcgt tacctgcttt ggctttctct
     9241 tctacgtcat agaagttatc gaacatcagc agtgtgtgag aaagtgcttt ggcagcgatc
     9301 ggtgccagtt tggcatcatc cagcgcgtcg atcagcggat gaatgttgta accaccctgc
     9361 atagtgccca gcagttcaat ggctttttcc ggagtcagca gtggggattt ggcttcacct
     9421 ttggcgacag cagccaggaa gcctgctttg acataggcgg cttcatcgac gccaggggga
     9481 acacggttgg ttaacagatc taacaggaat tcttcttcgc ccgcgggcgg gtttttcagc
     9541 agctctacaa gcgcggccat ttggtttgca tccaggggtt tgggcgcaat cccctcagcg
     9601 gcacgctcag ctacgtgctt acggtattct tctagcacga cggttctcct cgctctcatt
     9661 gtcatagtgc ggcaggcgat tctcttcacg ctcctgtgag acagcagttt gtagggtaaa
     9721 tgcccagtac cgcaacgggc agaatagcag gattttgacg atgtgttaat ctgtttacaa
     9781 aaaagcaaca taaaaaagtg gctgaatcgt taaggatggt ctagagatta tttccctccg
     9841 taaaaacttc gttaattcgc atggtgataa tcacctttct caacgaatcc caagattagt
     9901 caaaatttaa actacggcct ctttatactc ggattcacag cacctgcggg tggcagttcg
     9961 ccgaccattg cgatttcctt gagatccgaa ttatgaaaat gactttgccg tttaaacccc
    10021 atgtgctggc gctaatttgc agtgccgggc tctgtgccgc ctctgccggg ctatatataa
    10081 aaagccgcac agtggaagcg cctgtggaag cgcaatcgac acaacagact gcgcctgaca
    10141 tctccgcagt tacgcttcca gcaacggttt ccgtgcctcc tgtaacacct gccgtcgtca
    10201 aatccgcatt cagcactgct caaatagatc aatgggttgc acctgtcgcg ctataccccg
    10261 actctctgct ttcacaagtg ttaatggcat caacctatcc ggcaaacgtt gctcaagcag
    10321 tgcaatggtc gcacgataat ccacttaaac aaggcgatgc tgctattcag gcggtatctg
    10381 accagccctg ggacgccagc gtcaaatcac tggtggcctt tccacagttg atggcattga
    10441 tgggcgaaaa cccgcaatgg gtgcaaaacc tgggcgatgc ttttctggcg cagccgcagg
    10501 acgtgatgga ctcggtacag cgattgcggc aactggcgca acaaaccggc tcgctgaagt
    10561 catcaactga acaaaagatc atcacgacaa cgaaaaaagt cgtaccggta aatcagcccg
    10621 ccaacgcccc tgccacacaa tcaaataccg tttctacgtc cagccccgtc gtcgcagaac
    10681 ctgcaccgac cgtaataacc attgagccag ccaatccgga tgtggtttat attccgaact
    10741 acaacccaaa cgtggtttac ggcagttggg ccaataccgc ttatccacca gtttatctgc
    10801 cgccgccagc cggagaaccg tttgttgaca gctttgtgcg cggattcggc tatagcatgg
    10861 gcgttgctac cacatacgca ctattcagca gcatcgactg ggacgacgac gatcatgacc
    10921 atgaccatca tcatcatgac gatgatgatt atcatcacca cgatggcggt catcgtgacg
    10981 gtaatgactg gcaacacaac ggcgacaaca tcaatatcga cgtcaacaat ttcaaccgta
    11041 tcaccggtga gcatcttact gataagaata tggcatggcg gcacaatcca aactaccgta
    11101 atggtgtgac ctatcatgat caggatatgg caaagcggtt tcatcaaacc gatgtcaacg
    11161 gcggaatgag cgccacgcag ttacctgctc tatcgcgcga cagccagcgt caggcggcag
    11221 caagtcagtt tcagcaacga acacacgccg cccccgtcat tacgcgagat acccaacgtc
    11281 aggcagcggc acagcggttt aatgaagcgg aacactatgg caattatgac gacttccgcg
    11341 agttcagccg ccaccaacca ctgacccagc agcaaaagga cgccgctcgc cagcgttatc
    11401 agtctgcctc acctgagcag cgccaggcgg cccgtgagaa aatgcagact agcccgcaga
    11461 atcaacagca aagagatgca gcacgtcagc gtattcagtc cgcgtcgcct gagcagcgcc
    11521 aggtgtttaa ggaaaaagta cagcagcgcc cactgaacca agagcaacgt gataacgccc
    11581 gccagcgtgt tcaatcggca tcacctgaac aacgtccgtt ttttcgggag aaagttcagg
    11641 agagccgccc gcaacgtcta aacgacagta accgtactgc caggctgaat aacgagcagc
    11701 ggtcagcagt acgcgaacgt ctctctgaac gtggggcaag aagactggaa aggtaaattg
    11761 cagacgtaaa aaaagcggcg tggttagccg cttttttaat tgccggatgt tccggcaaac
    11821 gaaaaattac ttcttcttcg ctttcgggtt cggcaggtcg gtaatgctac cttcgaacac
    11881 ttctgccgcc aggcccacag actcgtgcag agtcgggtgc gcatggatgg tcagtgcgat
    11941 gtcttcagcg tcacaaccca tttcgattgc caagccgatt tcacccagca actcgccgcc
    12001 gttagtgccg acaatcgcac caccgatcac acggtgagat tctttgtcga aaatcagctt
    12061 ggtcataccg tctgcgcagt cggaagcgat agcacgacca gaagcagccc acgggaaggt
    12121 ggcggtttcg tagctgatgc ctttctcttt cgcttctttc tcagtcagac ctacccatgc
    12181 aacttctggt tcggtatagg cgatggacgg gataactttc ggatcgaagt aatgtttttt
    12241 accggcgata acttcagcgg caacgtgacc ttcgtgaaca cctttgtgtg ccagcattgg
    12301 ctgaccgacg atatcgccga tagcaaagat gtgcggtacg ttggtacgca gctgtttgtc
    12361 aacgcggatg aaaccacggt cgtcaacttc cacgccagct ttgcctgcgt cgaggttttt
    12421 accgttcggc acacgaccaa tcgctaccag cacggcgtcg taacgctgcg gttcagcggg
    12481 tgcttttttg ccttccatcg tcacataaat gccgtcttct ttcgcttcaa cggcggtaac
    12541 tttggtttcc agcatcaggt tgaatttctt gctgatacgc ttggtaaaga ctttaacgat
    12601 gtctttatca gctgccggga tgacctggtc gaacatttca accacgtcaa tctgtgaacc
    12661 cagcgcgtgg tatacggtgc ccatttccag accgatgata ccgccaccca ttaccagcag
    12721 gcgttctggt acttctttca gttccagcgc gtcagtggag tcccagatac gcggatcttc
    12781 atgcggaata aacggcagtt gaatcgggcg agaacccgct gcaatgatcg cgttgtcgaa
    12841 gttgatcacg gttttaccgt tctcaccttc aacttccagg gtgttagccc cggtgaattt
    12901 acccagaccg ttgaccactt tgactttgcg gcctttcgcc ataccagcca gaccaccggt
    12961 cagctgattg atcactttct ctttccaggt acgaatcttg tcgatatcgg ttttcggttc
    13021 gccgaagacg ataccgtgtt cagccagcgc tttggcttct tcgataactt ttgctacgtg
    13081 cagcagtgct ttagaaggga tacagccgac gttcaggcaa acaccgccaa gggtgttgta
    13141 acgttctacg attacggttt ccagacctaa atcagcgcaa cggaaggcag cggagtaacc
    13201 tgcggggcct gccccaagta ccacgacctg agttttgatt tcagtactca tcatgacctc
    13261 tatatattta tctccggcgg gtctgacgtt ttttttatga cgcccattat ccaccgggtc
    13321 gttctatccg tcggtatttt acaaaattgt taacaatttt taaacaacaa acggcaaccg
    13381 atttgtctat tcgctaataa cctcaataca ttcatgagat taccagaaaa aagccggccg
    13441 ttgggccggc tcttttactt acatcaccag acggcgaatg tcagacagcg tgttgttaat
    13501 gatggtaatg aaacgggcac catcagcacc gtcgatcacg cggtggtcga aggagagaga
    13561 aatcggcagc atcagacgcg gcacgaactc tttaccattc cacaccggct ccatcgcgga
    13621 cttggaaacg ccgaggatag ccacttccgg cgcattcaca atcggcgcga agtgggtagt
    13681 acccaggccg ccgatgctcg agatggtgaa gcaaccgccc tgcatttcgc ccgcagtcag
    13741 cttaccgtca cgcgctttct tagaaatagt catcagctcg cgagacagct cgatgatgcc
    13801 tttcttgttg acgtctttga ataccgggac aaccagaccg ttcggggtat ccaccgccac
    13861 accgatgttg atgtatttct tcagggtcag acgctgaccg tcttccgaca gcgaactgtt
    13921 gaagcgaggc atctgctcaa gagctgcagc aacggctttc atgatgaaga caaccggggt
    13981 gatcttcaca tccagcttac gtttcgctgc ttcttcgttc tgctgtttac ggaacgcttc
    14041 cagctcagtg atatcggttt tgtcgaagtg agtaacgtgc gggatcatca cccagttacg
    14101 gctcaggtta gcaccagaga ttttctgaat gcggcccagt tccacttctt cgatttcacc
    14161 aaacttgctg aagtccactt taggccacgg cagcatgcct gggataccgc cgccagttgc
    14221 cgccggagct gcttctgcac gtttgattgc ttctttcacg taagcctgaa cgtcttcgcg
    14281 caggatacga cctttacggc cagtgccctt cactttcgcc aggttaacgc caaactcgcg
    14341 tgccagacgg cggatcagcg gagtcgcgtg aacgtaagcg tcgttttcag caaattcaga
    14401 tttgccttcg gctttcgcag ccggtgctgc tgccggggct tcagcttttg ctgccggagc
    14461 cggcgctgcc gcttcctgtt tcgcaggagc tgccgcaggc gctgcgcctt caacttcgaa
    14521 gatcataatc agcgaaccag ttttcacttt atcgccaacg ttgactttca gttccttcac
    14581 gacgcctgca aacggagccg gaacttccat agaagcttta tcgccttcta cggtgatcag
    14641 tgactgttca gcggcaactt tgtcgcccac tttcaccatc acttcggtca cttcaacttc
    14701 gtcaccgccg atatccggaa cgttaacttc tttcacgcca gctgctggtg cagaggccgc
    14761 tgccggagcc gcttcctgtt tagccgccgg agctgccgcg ccagcttcac ccgcgacttc
    14821 gaagaccata atcagcgagc cggtagacac tttgtcaccc acgttcactt tgatctcttt
    14881 cacggtacct gcaaacggag ccggaacttc catagaagcc ttgtcgcctt ctacggtgat
    14941 cagcgactgt tcagcttcaa ctttgtcgcc cactttgacc aggatttcgg tcacttcaac
    15001 ttcgtcgctg ccgatatccg gaacgttaac gtcttttgcc gctgcagccg ctggtgctgc
    15061 tgccggagcg gcttctttct tctcttctgc ctgagcaggt gcagcgtctg ctgcaccgtc
    15121 ggcggaatcg aaaatcataa tcagtgcgcc ggtctgggtt ttatcgccaa cagagacttt
    15181 gatctcttta acgatacccg cctgcggaga tggtacttcc atagaggctt tgtcgccttc
    15241 tacggtgatc agcgactgtt cggcttcaac tttgtcgccc actttgacca ggatctcggt
    15301 gatttcaact tcatcagccc cgatgtccgg tactttgatt tcgatagcca ttattctttt
    15361 acctcttacg ccagacgcgg gttaacttta tctgcatcga tgttgaattt ggcgattgcg
    15421 tcagcaacca ctttcttatc gatttcgcca cgtttagcca gttcgcccag cgccgcaacc
    15481 acgacataag aagcatcaac ttcaaagtgg tgacgcaggt tctcacggct gtcggaacga
    15541 ccgaagccat cagtacccag tacgcggtag tcgtcagccg gtacgtaagt acggacctgc
    15601 tcagcgaaca gtttcatata gtcggtagat gccactgccg gagcgtcgtt catcacctga
    15661 gcgatatacg gaacgcgcgg agtttccagc gggtgcagca tgttccagcg ttcacaatcc
    15721 tgaccatcac gcgccagttc ggtgaaggaa gtcacgctat aaacgtcaga acctacgccg
    15781 taatctttcg ccaggatctc tgccgcttca cggacgtgac gcaggataga accggagccc
    15841 agcagctgaa ctttaccttt gctaccttca atggtttcga gtttgtagat acctttacgg
    15901 ataccttcct cagcaccttc cggcattgcc ggcatgtggt agttttcgtt cagcgtagtg
    15961 atgtagtagt aaacgttctc ttgtttttca ccgtacatac gctccagacc gtcatgcatg
    16021 atgacagcaa cttcgtaagc gtaagccggg tcgtaagaga tacagttcgg gatagtcagc
    16081 gactgaatat ggctgtgacc atcttcgtgc tgcagacctt caccgttcag ggtggtacga
    16141 ccggaagtac cgccgatcag gaagccacgc gcttgctggt cgccagccgc ccagcacaga
    16201 tcgccgatac gctggaaacc gaacatcgag taatagatgt agaacgggat catcggcaga
    16261 ttgttggtgc tgtaagaggt cgccgctgcc agccaggaac aacctgcgcc cagctcgttg
    16321 atcccttcct gcagaatctg acctttctcg tcttctttat agtaagcaac ctgctcgcgg
    16381 tcctgcgggg tgtactgctg accgttcggg ctgtaaatac caatctgacg gaacagacct
    16441 tccataccga aagtacgcgc ttcgtcggcg atgatcggta ccagacgatc tttgatcgac
    16501 ttgttcttca gcatcacgtt cagagcacga acgaaagcga tagtggtaga gatctctttg
    16561 ctctgctctt ccagcagcgc gccgaaatct tgcaggctcg gcagctccag cttctcggtg
    16621 aagttcggct ggcggcttgg cagataaccg tgcagtttct gacgttgagc gtgcagatag
    16681 gtatgctctt cagaaccttc cgggaaggtg atgtacggca gtttttcgat atctgcatca
    16741 gacaccggca cattgaaacg gtcgcggatg tgacgcacgc cgtccatgtt cattttctta
    16801 acctggtgcg cgatgttttt accttcagcc gcgtcgccca tgccgtaacc tttaatggta
    16861 tgagcaagga ttaccgtcgc tttgcctttg gtttcctgcg ctttcttgaa tgcagcgtag
    16921 attttcttcg gatcgtgacc accacggttc agtgcccaga tctgctcgtc agtccagtct
    16981 gcaaccagtg ctgcggtttc aggatattta ccgaagaagt gttcgcgaac atacgcacca
    17041 tctttcgatt tgaaggtctg gtaatcgccg tcaacggttt cgttcatcag ctggatcagt
    17101 ttaccgctgg tatctttacg cagcagttcg tcccaacggc taccccacat cactttgatc
    17161 acgttccagc cagcaccttc gaagatgcct tccagttcgt tgatgatctt gccgttaccg
    17221 gtgaccgggc cgtcaagacg ctgcaggtta cagttgataa cgaagaccag attatccagt
    17281 ttttcacggg tagcgatggt gatcgcacct ttggattccg gttcgtccat ttcaccgtcg
    17341 ccgaggaacg cgtaaacggt ttgtttagag gtatctttca ggccacggtg ttccagatat
    17401 ttcaggaatt tagcctggta aatagcacca atcggaccca gccccataga tacggtcggg
    17461 aactgccaga attccggcat cagtttcggg tgcggatagg aagagaggcc attgccgtga
    17521 acttcctgac ggaagttatc cagctgctcc tgagtcagac gaccttccag gaaagcacga
    17581 gcgtaaacgc ctggggagat gtggccctgg aagtaaacca ggtcgccgcc atcctgctcg
    17641 ttgcgtgcac ggaagaagtg gttaaagcac acatcataaa tggttgcgga agactggaag
    17701 gacgccatgt ggccgcccag ttcgaggtct tttttcgacg cacgcagcac cgtcatgatg
    17761 gcgttccagc ggatagctga acgaatacgg cgttccagtt ccagattacc cggatactcc
    17821 ggttgttctt caacggggat ggtgttgatg tagttgctga taccggtgcc tgcagctacg
    17881 ttgactccgc ctttgcgggc ttcagcaagc agttggtcga tcagatactg agcacgctca
    17941 acaccttctt cacggatgac cgattcgatc gcctggagcc agtcgcgagt ttcgatcgga
    18001 tccacgtcat ttgggaaacg ttctgacatg ggttattcct tatctatcta ataacgttga
    18061 gttttctgga acctgtccca ttgaactctc gccggaaagc tcaataagac aggttctacg
    18121 tttagttgcc gcgcttttat atgcgcttga tttacaacat cttctggata atttttacca
    18181 gaaaaatcac taattctttc gttgctccag acgacgcaga gaacgctcac ggcggctctc
    18241 ttcacggctt ctgtcgagca aaatttcttc gataaaggcc agatggcgat gcgatgcttc
    18301 gcgcgcttct tccggcttac cggccataat cgcttcaaat atgcgggtgc ggtgactact
    18361 caccagcggc agcatctcgc gacgcgaata gagcaattcg aagttctggc ggacattctg
    18421 agccaacatc ggctccatac accttagcag atgaagcaga accacattgt gggccgcttc
    18481 ggtgacggca atctgatact ggagtacggc atttgattcc gcgtccagat cgccagactg
    18541 ctgcgccagc tctatggcat ggtggagttc acggatgcgt tccttgtctt catcggtact
    18601 acgcagcgcg gcgtaataag cggcgatacc ttccagggcg tgtcgtgttt cgagcaagtc
    18661 atactgtgat tcaggatggt cggagagcag ctccaccagc ggatcgctga agctttgcca
    18721 gaggctgctc tggacaaaag tgccgccacc ctggcgacga agcaacaagc ccttcgcttc
    18781 gaggcgttga atcgcctcac gcaaagaggg acgggagacg tcgaactgtt ttgccagttc
    18841 gcgttccggt gggagttttt cgcccgggcg gagagtgcct tcgaggatca aaaactccag
    18901 ttgctgctca atcacatcgg agagttttgg ttggcggatt ttgctgtagg ccatgagttc
    18961 ctgtcttaag ccacttgccg aagtcaattg gtcttaccaa tttcatgtct gtgacgctaa
    19021 agtaacaaag tattcacctt atgtccatac aggttttgat tgaaatcatg aaactgtgca
    19081 cattttaaca acttgacata tataacgttt caaagttgta actatgcaca aatgttaaat
    19141 ttacccatca aggggtgacc ttaacagaat tgaaacgaaa aaatatttcc tctgaaccga
    19201 ttcaccaagc aacaaaacgc ataacaattc aattatgtga attaatatgt aaaatgaatg
    19261 ttcagggaag gagagaaagg tagtatttac ctatgttttc tttttacatt gcttattatc
    19321 actgcaatcg ccaccataac cttctcgtag cgaaaatcat cacttaacca gaacgtgatg
    19381 attttcaggt gacagattct gtttagagtt cattctgaat ctttgaacaa aggcaatcct
    19441 tgcgaacgcc gccattcttt gacgattttc aggatgtttt caggttcatc atctcccttc
    19501 tttgcaggat agtagataag atcgctcatc aacggatgtt gtgtaattct ggtaagatgt
    19561 tcttctagat catcccaata tttgtcaagc tcttcccctt ttaattgagc gttatccccg
    19621 gttggatttg taaactcgcc aagcaactca atgaattccc gctctgtgta atcttctatt
    19681 ttatctttaa aattatacat ttaactatct cctgtaataa atttcatcat gcaaatgagg
    19741 tgtgccctcc ttaaattatc aatatcgtaa attccgccac tata
//
LOCUS       DAHHTG010000080        19501 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.080, whole genome shotgun sequence.
ACCESSION   DAHHTG010000080 DAHHTG010000000
VERSION     DAHHTG010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19501)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19501)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19501
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.080"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            complement(327..1997)
                     /gene="betA"
                     /locus_tag="NBI74_002953"
     CDS             complement(327..1997)
                     /gene="betA"
                     /locus_tag="NBI74_002953"
                     /EC_number="1.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline dehydrogenase"
                     /protein_id="HCD1933247.1"
                     /translation="MQFDYIIIGAGSAGNVLATRLTEDPNTTVLLLEAGGPDYRFDFR
                     TQMPAALAFPLQGKRYNWAYETEPEPFMNNRRMECGRGKGLGGSSLINGMCYIRGNAL
                     DLDNWAQEPGLENWSYLDCLPYYRKAETRDVGENDYHGGDGPVSVITSKPGVNPLFEA
                     MIEAGMQAGYPRTDDLNGYQQEGFGPMDRTVTPHGRRASTARGYLDQAKSRPNLTIRT
                     HAMTDHIIFDGKRAVGVEWLEGDSTIPTRAAANKEVLLCAGAIASPQILQRSGVGNAE
                     LLAEFDIPLVHELPGVGENLQDHLEMYLQYECKEPVSLYPALQWWNQPRIGAEWLFGG
                     TGVGASNHFEAGGFIRSREEFAWPNIQYHFLPVAINYNGSNAVKEHGFQCHVGSMRSP
                     SRGHVRIKSRDPHQHPGILFNYMSHEQDWQEFRDAIRITREIMHQPALDQYRGREISP
                     GVECQTDEQLDEFVRNHAETAFHPCGTCKMGYDEMAVVDGEGRVHGLEGLRVVDASIM
                     PQIITGNLNATTIMIGEKIADMIRGKEALPRSTAGYFVANGMPVRAKK"
     gene            complement(2011..3483)
                     /gene="betB"
                     /locus_tag="NBI74_002954"
     CDS             complement(2011..3483)
                     /gene="betB"
                     /locus_tag="NBI74_002954"
                     /EC_number="1.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="betaine-aldehyde dehydrogenase"
                     /protein_id="HCD1933248.1"
                     /translation="MSRMAEQQLYIHGGYTSATSGRTFETINPANGNVLATVQAAGRE
                     DVDRAVKSAQQGQKIWAAMTAMERSRILRRAVDILRERNDELAKLETLDTGKAYSETS
                     TVDIVTGADVLEYYAGLIPALEGSQIPLRETSFVYTRREPLGVVAGIGAWNYPIQIAL
                     WKSAPALAAGNAMIFKPSEVTPLTALKLAEIYSEAGLPDGVFNVLPGVGAETGQYLTD
                     HPGIAKVSFTGGVASGKKVMANSAASSLKEVTMELGGKSPLIVFDDADLDLAADIAMM
                     ANFFSSGQVCTNGTRVFVPTKCKAAFEQKVLARVERIRAGDVFDPQTNFGPLVSFPHR
                     DNVLRYIAKGKEEGARVLCGGNVLKGDSFDNGAWVAPTVFTDCSDDMTIVREEIFGPV
                     MSILIYESEDEVIRRANDTDYGLAAGIVTADLNRAHRVIHQLEAGICWINTWGESPAE
                     MPVGGYKHSGIGRENGVMTLQSYTQVKSIQVEMAKFQSIF"
     gene            complement(3497..4084)
                     /gene="betI"
                     /locus_tag="NBI74_002955"
     CDS             complement(3497..4084)
                     /gene="betI"
                     /locus_tag="NBI74_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308386.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator BetI"
                     /protein_id="HCD1933249.1"
                     /translation="MPKLGMQSIRRRQLIDATLEAINEVGMHDATIAQIARHAGVSTG
                     IISHYFRDKNGLLEATMRDITSQLRDAVLNRLHALPQGSAELRLQAIVGGNFDETQVS
                     SAAMKAWLAFWASSMHQPMLYRLQQVSSRRLLSNLVSEFRRELPRQQAQEAGYGLAAL
                     IDGLWLRAALSGKPLDKSLAHSLTRHFITQHLPTD"
     gene            4213..6246
                     /gene="betT"
                     /locus_tag="NBI74_002956"
     CDS             4213..6246
                     /gene="betT"
                     /locus_tag="NBI74_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline BCCT transporter BetT"
                     /protein_id="HCD1933250.1"
                     /translation="MTDLSHSREKDKINPVVFYTSAGLILLFSLTTILFRDFSALWIG
                     RTLDWVSKTFGWYYLLAATLYIVFVVCIACSRFGSVKLGPEQSKPEFSLLSWAAMLFA
                     AGIGIDLMFFSVAEPVTQYMQPPEGAGQTIEAARQAMVWTLFHYGLTGWSMYALMGMA
                     LGYFSYRYNLPLTIRSALYPIFGKRINGPIGHSVDIAAVIGTIFGIATTLGIGVVQLN
                     YGLSVLFDIPDSMAAKAALIALSVIIATISVTSGVDKGIRVLSELNVALALGLILFVL
                     FMGDTSFLLNALVLNVGDYVNRFMGMTLNSFAFDRPVEWMNNWTLFFWAWWVAWSPFV
                     GLFLARISRGRTIRQFVLGTLIIPFTFTLLWLSVFGNSALYEIIHGGAAFAEEAMVHP
                     ERGFYSLLAQYPAFTFSASVATITGLLFYVTSADSGALVLGNFTSQLKDINSDAPGWL
                     RVFWSVAIGLLTLGMLMTNGISALQNTTVIMGLPFSFVIFFVMAGLYKSLKVEDYRRE
                     SANRDTAPRPLGLQDRLSWKKRLSRLMNYPGTRYTKQMMETVCYPAMEEVAQELRLRG
                     AYVELKNLPPEEGQQLGHLDLLVHMGEEQNFVYQIWPQQYSVPGFTYRARSGKSTYYR
                     LETFLLEGSQGNDLMDYSKEQVITDILDQYERHLNFIHLHREAPGHSVMFPDA"
     gene            7124..8212
                     /gene="pdeL"
                     /locus_tag="NBI74_002957"
     CDS             7124..8212
                     /gene="pdeL"
                     /locus_tag="NBI74_002957"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator/c-di-GMP
                     phosphodiesterase PdeL"
                     /protein_id="HCD1933251.1"
                     /translation="MNSCDFRVFLQEFGTTVHLSLPGSVSEKERLLLKLLMQGMSVTE
                     ISQYRNRSAKTISHQKKQLFEKLGIQSDITFWRDIFFQYNPEIISATGNNSHKYINDN
                     HYHHIVTPEAISLALENHEFKPWIQPVFCAQTGVLTGCEVLVRWEHPQTGMIPPDQFI
                     PLAESSGLIVIMTRQLMKQTADILMPVKHLLPDNFHIGINVSAGCFLAAGFEKECLNL
                     VKKLGNDKIKLVLELTERNPIPVTPEARAIFDSLHQHNITFALDDFGTGYATYRYLQA
                     FPVDFIKIDKSFVQMASVDEISGHIVDNIVELARKPGLSIVAEGVETQEQADLMIGKG
                     VHFLQGYLYSPPVPGNKFISEWVMKAGG"
     gene            complement(8254..9186)
                     /locus_tag="NBI74_002958"
     CDS             complement(8254..9186)
                     /locus_tag="NBI74_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCD1933252.1"
                     /translation="MNSIFTEENLLAFTTAARFGSFSKAAEELGLTTSAISYTIKRME
                     TGLDVVLFTRSTRSIELTESGRYFFRKATDLLNDFHAIKRSIDTISQGIEARVRICIN
                     LLLYTPKHTARLLQVLKKQFPTCQITVTTEVYNGVWDAIINNQANIAIGAPDTLLDGG
                     GIDYTEIGAIRWAFAIAPDHPLAFVPEPIAESQLRLYPNIMVEDTAHTINKKVGWLLH
                     GQESILVPDFNTKCQCQILGEGIGFLPDYMVREAMAQSLLVTRQIHNPRQDSRMLLAT
                     QHSATGQVTQWIKKQFAPNGILTGIYQDLLHREN"
     gene            complement(9278..9775)
                     /locus_tag="NBI74_002959"
     CDS             complement(9278..9775)
                     /locus_tag="NBI74_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HCD1933253.1"
                     /translation="MNGLTATGVTVGICAGLWQLVSSHVGRSQGWELLGTIGFVAFCS
                     FYAAGGGKSGFIRSLAVNYSGMVWAFFAALAAGWLASVSGISGFWASVITTVPFSAGV
                     VWQGRFWLLSFIPGGFLGMTLFFASGMNWTVTLLGFLAGNCVGIISEYGGQKLSEATT
                     KRDGY"
     gene            10033..10638
                     /locus_tag="NBI74_002960"
     CDS             10033..10638
                     /locus_tag="NBI74_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HCD1933254.1"
                     /translation="MTIKNLPADYLLAAQQGDIDKVKTCLALGVDINTCDRQGKTAIT
                     LASLYQQYACVQALIDAGANINKQDLTCLNPFLISCLNDDLTLLRIILPAKPDLNCVT
                     RFGGVGLTPACEKGHLSIVKELLANTEINVNQTNHVGWTPLLEAIVLNDGGIKQQAIV
                     QLLLEHGASPHLTDKYGKTPLKLARERGFEEIAQLLIAAGA"
     gene            10678..11541
                     /locus_tag="NBI74_002961"
     CDS             10678..11541
                     /locus_tag="NBI74_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
                     /protein_id="HCD1933255.1"
                     /translation="MWALTADADFLAQRGQGQVEQVFARAVNIALPARQQLLTLLCEE
                     YDNAPNSCRLALTHFNGLFRHDDKVQFDDQGITIGQHHHIEMSHCQRWLSPTLQMTAV
                     NFHLIAWQQWYDIIHQHLGENETLFNYRGDNPFYQALNKELHIKRRAVIQAVNDKQNI
                     ASAVASMMGLGIGLTPSADDYLTGLVLILFISGHPAEKYKEEFYLGLQRGRNNTTLLS
                     AITLEAALQQRCRENIHRFIHNIIYDIPGNATQAIEKIKHIGSSSGCDMLYGMADGCA
                     LSQTYGGNYVS"
     gene            11531..13078
                     /gene="fdrA"
                     /locus_tag="NBI74_002962"
     CDS             11531..13078
                     /gene="fdrA"
                     /locus_tag="NBI74_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase FdrA"
                     /protein_id="HCD1933256.1"
                     /translation="MSVKIVIKPNTYFDSVSLMSISTRANKLDGVEQAFVAMATEMNK
                     GVLKNLGLLTPELEQAKNGDLMIVINGKSGVDNEQLLAEIEELFNTKAQSGSHEARYG
                     TIASAKKHIPESNLAVISVNGLFAAREARQALQNDLNVMLFSDNVSVEDELALKQLAH
                     EKGLLMMGPDCGTAIINGAALCFGNAVRRGNIGIVGASGTGSQELSVRIHEFGGGVSQ
                     LIGTGGRDLSEKIGGLMMLDAISMLENDPQTEIIVLISKPPAPAVARKVLERARACRK
                     PVVACFLGRGETPVDEQGLQFARGSKEAALKAVMLSGVKQEHLDLHTLDQPLIADVRA
                     RLQPQQKYIRGLFCGGTLCDETLFAVMEKHGDVYSNIQPDPEFRLQDINRSIKHTFLD
                     FGDDDFTNGKPHPMIDPTNRISRLIEEARDPEVAVIVMDFVLGFGSHEDPVGSTIEAI
                     KEAKAIAAAEGRELIILAYVLGTDLDTPSLEQQSQMLLDAGVILASSSTNTGLLAREF
                     ICKGEEA"
     gene            13078..14496
                     /locus_tag="NBI74_002963"
     CDS             13078..14496
                     /locus_tag="NBI74_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HCD1933257.1"
                     /translation="MSQSLFSQPLNVINVGIAMFSDDLKKQHVEVTQLDWTPPGQGNM
                     QVVQALDNIADSPLADKITAANQQALERIIQSHPVLIGFDQAINVVPGMTAKTILHAG
                     PPVTWEKMCGAMKGAVTGALVFEGLAKDLDEAAELAASGEITFSPCHEHDCVGSMAGV
                     TSASMFMHIVKNKTYGNIAYTNMSEQMAKILRMGANDQSVIDRLNWMRDVQGPMLRDA
                     MKIIGEIDLRLMLAQALHMGDECHNRNNAGTTLLIQALTPGIIQAGYSVEQQREVFEF
                     VASSDYFSGPTWMAMCKAAMDAAHGIEYSTVVTTMARNGVEFGLRVSGLPGQWFTGPA
                     QQVIGPMFAGYKPEDSGLDIGDSAITETYGIGGFAMATAPAIVALVGGTVEEAIDFSR
                     QMREITLGENPNVTIPLLGFMGVPSAIDITRVGSSGILPVINTAIAHKDAGVGMIGAG
                     IVHPPFACFEKAILGWCERYGV"
     gene            14649..15599
                     /locus_tag="NBI74_002964"
     CDS             14649..15599
                     /locus_tag="NBI74_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase family protein"
                     /protein_id="HCD1933258.1"
                     /translation="MKELVVVAIGGNSIIKDNASQSIEHQAEAVKAVADTVLEMLASD
                     YDIVLTHGNGPQVGLDLRRAEIAHEREGLPLTPLANCVADTQGGIGYLIQQALNNRLA
                     RHGEKKAVTVVTQVEVDKNDPGFAHPTKPIGAFFSESQRDELQKANPDWRFVEDAGRG
                     YRRVVASPEPKRIVEAPAIKALIQQGFVVIGAGGGGIPVVRTEAGDYQSVDAVIDKDL
                     STALLAREIHADILVITTGVEKVCIHFGKPQQQALDRVDIATMTRYMQEGHFPPGSML
                     PKIIASLTFLEQGGKEVIITTPECLPAALRGETGTHIIKT"
     gene            15609..16991
                     /locus_tag="NBI74_002965"
     CDS             15609..16991
                     /locus_tag="NBI74_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HCD1933259.1"
                     /translation="MKENNSRREFLSQSGKMVTAAALFGTSVPLAHAGVAGTLNCEAN
                     NTMKITDPHYYLDNVLLETGFDYENGVAVQTRTARQTVEIQDGKIVALRENKQHPDAT
                     LPHYDAGGKLMLPTTRDMHIHLDKTFYGGPWRSLNRPAGTTIQDMIKLEQKMLPELQP
                     YTQERAEKLIDLLQSKGTTIARSHCNIEPVSGLKNLQNLQAVLARRQTGFECEIVAFP
                     QHGLLLSKSESLMREAMQAGAHYVGGLDPTSVDGAMEKSLDTMFQIALDYDKGVDIHL
                     HETTPAGVAAINYMVETVEKTPQLKGKLTISHAFALATLNEQQVDKLAHRMVAQQISI
                     ASTVPIGTLHMPLKQLHDKGVKVMTGTDSVIDHWSPYGLGDMLEKANLYAQLYIRPNE
                     QNLSRSLFLATGDVLPLNEKGERVWPKAQDDASFVLVDASCSAEAVARISPRTATFHK
                     GQLVWGSVAG"
     gene            17368..18417
                     /gene="yahK"
                     /locus_tag="NBI74_002966"
     CDS             17368..18417
                     /gene="yahK"
                     /locus_tag="NBI74_002966"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent aldehyde reductase YahK"
                     /protein_id="HCD1933260.1"
                     /translation="MKIKAVGAYSAKQPLEPMDITRREPGPHDVKIEIAYCGVCHSDL
                     HQVRSEWAGTVYPCVPGHEIVGRVVAVGDQVEKHAPGDLVGVGCIVDSCKHCEECEDG
                     LENYCDHMTGTYNSPTPDEPGHTLGGYSQQIVVHERYVLRIRHPQEQLAAVAPLLCAG
                     ITTYSPLRHWQAGPGKKVGVVGIGGLGHMGIKLAHAMGAHVVAFTTSESKREAAKALG
                     ADEVVNSRNADEMVAHVKSFDFILNTVAAPHNLDDFTTLLKRDGTITLVGAPATPHKS
                     PEVFNLIMKRRAIAGSMIGGIPETQEMLDFCAEHGIVADIEMIRADQINEAYERMLRG
                     DVKYRFVIDNRTLTD"
     gene            19039..19283
                     /locus_tag="NBI74_002967"
                     /pseudo
     CDS             19039..19283
                     /locus_tag="NBI74_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414861.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(19300..>19501)
                     /locus_tag="NBI74_002968"
     CDS             complement(19300..>19501)
                     /locus_tag="NBI74_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HCD1933261.1"
                     /translation="PTWARLMAWAGIVLTSIIWRVFLSQAFSLPAVRRAYGRMQRVAS
                     RVIGAIIGVFALRLIYEGVTQR"
BASE COUNT         4694 a         4899 c         5220 g         4688 t
ORIGIN      
        1 tccatgtggt tttggtctga cttatttttg tactcgttac aagaaccctt ttcgtatatg
       61 ctaaccgtga gcgggaatga acaggtaacc atagccagga acattagcgg cgtaattctt
      121 aagctgagtg gtatttcatc tgtagtttat ctgtgtatgc atagcacatt catcagccag
      181 attgagcaag tcagcttaga atttgctggc gagattgtca cgatgaagca tgagagttac
      241 ctcagtgttt tatataagga ttcggtcctc atagtagaac ggtaactctc aatctgatag
      301 gttcctgagt cagttcacat cacgactcat tttttcgctc tcaccggcat cccatttgcc
      361 acaaaatatc ccgccgtgct cctcggcaac gcttccttcc cacgaatcat atccgctatt
      421 ttctcgccaa tcataatcgt cgtggcgttc aaattgccgg tgataatttg cggcataatt
      481 gacgcatcca ccacgcgcag gccttccagc ccgtgtacgc ggccttcgcc gtcaaccaca
      541 gccatctcgt cataacccat tttgcaggta ccgcacggat gaaacgcggt ttcggcgtgg
      601 ttacgcacga actcatcgag ctgctcatcc gtctggcatt cgacaccagg gctgatttcg
      661 cggccacggt attgatccag cgcgggttga tgcatgatct cgcgtgtgat gcgaattgcg
      721 tcgcggaact cctgccagtc ctgctcgtgc gacatgtagt taaacagaat tcccggatgc
      781 tggtgcgggt cgcgggattt aatccgcaca tgcccacggc ttggcgagcg catcgatccg
      841 acatggcatt ggaagccgtg ctctttcact gcgttcgagc cgttatagtt aatcgccact
      901 ggcaggaaat ggtactgaat attcggccac gcaaattcct cacggctgcg aataaatccg
      961 cctgcttcaa agtggttgct ggcaccaacg ccggtgccgc caaacagcca ctccgcaccg
     1021 attctcggct gattccacca ctgcagggca gggtagaggg aaaccggttc tttgcactcg
     1081 tattgcagat acatctccag gtgatcctga agattttcgc cgacgccggg taattcatgc
     1141 accagcggaa tatcaaactc cgccagcagt tcagcgttgc cgacgccgga gcgttgcagg
     1201 atctgcggtg aagcaatcgc gcctgcacat aacagcactt ctttattcgc cgccgcgcgg
     1261 gtcggaatgg tgctgtcgcc ttccagccac tcgacgccca ccgcgcgttt gccgtcaaaa
     1321 atgatgtgat cggtcatggc gtgagtacga atggtcaggt tagggcgcga tttggcctga
     1381 tcgagatagc cacgcgcggt gctggcgcga cggccatgcg gcgtgacggt gcgatccatc
     1441 gggccaaaac cttcttgctg atagccgttg agatcgtccg tgcgtgggta gcctgcctgc
     1501 atgcccgctt caatcatcgc ttcaaacagc ggattgacgc cgggtttgga ggtgatgacg
     1561 ctcaccgggc catcgccacc gtggtaatcg ttctcgccca catcgcgagt ctcggccttg
     1621 cggtagtagg gcaggcagtc gagataactc cagttctcca gaccaggttc ttgtgcccag
     1681 ttatcgagat ccagcgcatt gccacggatg tagcacatac cgttgatcag cgatgaaccg
     1741 cccagacctt tgccgcgtcc gcactccatg cggcggttgt tcataaacgg ttcgggttcc
     1801 gtctcatagg cccagttgta gcgtttgccc tgtagcggga acgccagcgc ggcgggcatc
     1861 tgggtgcgga agtcaaagcg atagtccggg ccgcctgctt caagcagcag cacggttgtg
     1921 ttcggatctt cagtcagacg ggtagcgaga acgttgccgg ctgagccggc accaataatg
     1981 atgtagtcaa attgcaaata aacctcctgg ttagaatatg gactggaatt tagccatctc
     2041 aacctggatg gacttcacct gggtgtaact ctggagcgtc atcacgccgt tctcgcgacc
     2101 aatgccggag tgtttgtagc cgccaacggg catctctgcc ggggattcgc cccaggtgtt
     2161 gatccagcaa atacccgctt ccagctgatg aatgacgcga tgcgcgcggt tcaggtccgc
     2221 cgtcacgata cccgccgcca gaccgtagtc ggtatcgtta gcgcggcgaa tgacttcgtc
     2281 ttccgactcg tagatcagaa tggacatcac cggcccgaaa atctcttctc gcacaatggt
     2341 catatcgtcg ctgcaatcgg tgaacacggt cggtgcaacc catgcgccgt tatcgaagct
     2401 atcccccttc agtacattgc cgccgcacag tacgcgcgcg ccttcctctt tgcctttggc
     2461 gatatagcgc agcacgttat cgcgatgcgg gaagctgacc agcgggccga agttagtttg
     2521 cggatcgaaa acgtcgcccg cgcgaatgcg ctcaacgcgc gccagaacct tttgttcaaa
     2581 tgcggctttg catttcgtcg gaacgaagac gcgagtgccg ttggtacaca cctgaccgga
     2641 gctgaagaaa ttcgccatca tggcgatatc ggcggcgaga tcgagatccg catcgtcgaa
     2701 aacgatcagc ggtgatttac cgcccagctc catggtcact tctttcaggg aagaggccgc
     2761 cgagttagcc atcacttttt tgccgctggc gacaccgccg gtaaatgaca ctttggcaat
     2821 gcccggatga tcggtcagat attgcccggt ctctgcgccc acgcccggca acacgttaaa
     2881 tacaccgtcc ggcaggcccg cttcgctgta aatttcagcc agctttaacg cggtaagcgg
     2941 ggtgacttcg ctcggtttga agatcatcgc gttacctgcc gccagcgcgg gggcggattt
     3001 ccacaaagca atctggatcg ggtagttcca tgcgccaatc cccgccacca cacccagcgg
     3061 ttcgcggcgg gtataaacaa atgacgtctc gcgcaacggg atctggctgc cttccagcgc
     3121 cgggatcagc ccggcatagt actccagcac gtccgcaccg gtaacgatat cgacggttga
     3181 ggtttccgaa tatgcttttc cggtgtcgag ggtttccagt tttgcgagtt cgtcattgcg
     3241 ttcacgcaga atatcaacgg cccgacgcag aatacgcgag cgttccatag cggtcatcgc
     3301 cgcccagatt ttttgccctt gttgggcact cttcacggcg cgatcgacat cctcacgccc
     3361 ggcggcctgc acggtcgcca gcacgttacc gttggccggg ttaatggtct cgaaggtgcg
     3421 accgctggtg gcggaggtat aaccaccatg tatataaagc tgctgttctg ccattcggga
     3481 catcacgtct cctcggttaa tcggtgggta aatgctgagt aataaagtga cgggtcagcg
     3541 agtgtgcgag agatttatcc agcggtttgc cgctcagtgc cgcgcgcagc cataatccgt
     3601 caattagcgc ggcaaggccg tagcccgctt cctgtgcctg ttggcgcggt aactcgcggc
     3661 gaaattcgct caccagattc gacaataagc ggcggctact gacctgctgc aagcgataga
     3721 gcatcggctg atgcatactg ctggcccaga aggccagcca ggctttcatc gccgcactgc
     3781 tcacctgggt ttcatcgaag tttccgccaa caatcgcctg taatcgcagc tctgcactgc
     3841 cctgcggaag tgcatgtaat cgattcaaaa ccgcgtcacg cagctgactg gtgatatcgc
     3901 gcatggttgc ttccagcaga ccatttttgt ccctgaaata gtggctgatg atccccgtag
     3961 aaacgcctgc atggcgggcg atctgcgcga tcgttgcatc gtgcatgccc acttcattta
     4021 ttgcttccag cgtggcgtcg atcagttgtc tgcgccggat cgactgcatt cccaatttgg
     4081 gcattttcgc cactccattc atcagcgttg tttatctatt aaagcggtta ttgattggac
     4141 gttcaatata aaatgtgtct taattgttac taatttgatt ttaaatggta acaataacag
     4201 tggggatact ggatgacaga cctttcacac agcagggaaa aggacaaaat caatccggtg
     4261 gttttttaca cttccgccgg actgattttg ttgttttccc tgacaacgat cctgtttcgc
     4321 gacttctcgg ccctgtggat tggccgcacg ctggactggg tttctaaaac cttcggttgg
     4381 tactatctgc tggcggcaac gctctatatt gtctttgtgg tctgtatcgc ttgttcgcgt
     4441 tttggttcgg tgaagctcgg gccagaacaa tcaaaaccgg aattcagttt gctgagttgg
     4501 gcggcgatgc tgtttgctgc cgggatcggt atcgacctga tgttcttctc cgtagccgaa
     4561 ccggtaacgc agtatatgca gccgccggaa ggcgcgggac agacgattga ggccgcgcgt
     4621 caggcgatgg tctggacgct gtttcactac ggcttaaccg gctggtcgat gtatgcgctg
     4681 atgggcatgg cgctcggata ctttagctat cgttataatt tgccgctcac tatccgctcg
     4741 gcgctgtacc cgatcttcgg taaacggatt aacgggccga taggtcactc agtggatatt
     4801 gctgcggtga tcggcaccat cttcggtatt gccactacgc tcggtatcgg tgtggtgcag
     4861 cttaactatg gcttgagcgt gctgtttgat attcccgatt cgatggcggc gaaagcggca
     4921 ctgatcgcct tgtcggtgat catcgccact atctcggtga catccggtgt cgataagggc
     4981 attcgcgtgt tatcggaact caacgttgcg ctggcgctgg gattgattct gttcgtgttg
     5041 tttatgggcg acacctcgtt cctgcttaat gcactggtgc tgaacgttgg cgactatgtg
     5101 aatcgcttta tgggcatgac gctcaatagt tttgccttcg accgtccggt tgagtggatg
     5161 aataactgga cgctcttctt ctgggcatgg tgggtggcat ggtcgccgtt tgtcggcttg
     5221 ttcctggcgc gtatctcgcg tgggcgtacc attcgccagt tcgtgctggg cacgttgatt
     5281 attccgttta ccttcacgct gttatggctc tcggtattcg gcaatagcgc gctgtatgaa
     5341 atcatccacg gcggcgcggc atttgccgag gaagcgatgg tccatccgga gcgcggcttc
     5401 tacagcctgc tggcgcagta tccggcgttt acctttagcg cctccgtcgc caccattact
     5461 ggcctgctat tttatgtgac ctcggcggac tctggtgcgc tggtgctggg gaatttcacc
     5521 tcgcagctta aagatatcaa cagcgacgcc cccggctggc tgcgtgtctt ctggtcggtg
     5581 gcgattggcc tgctgacgct aggcatgctg atgaccaacg ggatatccgc gctgcaaaac
     5641 accacggtga ttatgggcct gccgttcagc tttgtgatct tcttcgtgat ggcggggttg
     5701 tataaatctc tgaaggtaga agattaccgc cgtgaaagtg ccaaccgcga taccgcgccg
     5761 cgaccgctgg ggcttcagga tcgcctgagc tggaaaaaac gtctctcgcg cctgatgaat
     5821 tatccgggca cgcgttacac taaacagatg atggagacgg tctgttatcc ggcaatggaa
     5881 gaagtggcgc aggaactgcg gttgcgcggc gcgtacgtgg agctaaaaaa cctgccgccg
     5941 gaagagggac aacagttggg gcatctggat ttgttggtgc atatgggcga agagcaaaac
     6001 tttgtctatc agatttggcc acagcaatat tcggtgccgg gctttaccta ccgcgcacgt
     6061 agcgggaaat cgacctacta ccggctggaa accttcctgt tagaaggcag ccagggcaac
     6121 gacctgatgg actacagcaa agagcaggtg atcaccgata ttctcgacca gtacgagcgg
     6181 caccttaact ttatccatct ccatcgtgaa gcgccgggcc atagcgtgat gttcccggac
     6241 gcgtgattgt tattgcataa aaccgcgcca tgtctgcata tggcgcggtt tctcttacct
     6301 cttgatacgt tatatctgtg tacagttaag cccttagtat cgattgatga ttaccaggca
     6361 acagataata aaaaagaaaa gaactattga agcccaaaac ctacgcttgg actgttgtga
     6421 atgttcaata agcttagtct tatttaatgt aaatattggt gctaatttga aatgacgctt
     6481 tattcatggg aaacgcgtag cgcaaatcaa ctaaaatcaa tgtaaccgtt tgttgtttaa
     6541 ggtttaattg tctgtgtctg atattttatt gattgtttta aatattgttt ttattgtcat
     6601 tgttataatg ttggctatct attgctgaat ggattcagtc ttaaaaagca ggttgttaag
     6661 ggataggcat agagtaatga cgtgtatgca taaccaacat ctttactcat tatgtcattg
     6721 aatgttgacg ctatgtgttt atgaggttga ggtattttca gttgatctgg attgttaaat
     6781 tcatataatg cgcctttact catgaatgga cgtcaatacg tagtgggaaa ttataaatat
     6841 cgaaatagtc caactacttc tttattacca aaaatgagta tctgaatttt aatattgcat
     6901 tcttgcgtga ttatctcctg agtttgactt gtgattacct ttttaaggta tttagcgtaa
     6961 ctgtttttga gcgagcatca gaggtaaaga taatcttctt gatagtgatg tgggatgtta
     7021 tacgtatggc atcgctaatg tttatggtta ccccttatgt gtgctcagga atccacaggt
     7081 aatcactcat actgaacagc gataaaagat aaaaggtgtg ttcatgaatt catgtgattt
     7141 tcgtgttttt ctgcaagagt tcggtacaac ggttcatttg tcattgcctg gtagcgtatc
     7201 cgagaaagaa cgactgctac tcaagctgct gatgcaggga atgtctgtaa cagaaatatc
     7261 acagtacaga aatcgcagtg caaagaccat ttcacatcaa aagaaacagc tctttgagaa
     7321 actggggatt cagagcgata ttactttctg gcgggatatt ttctttcagt acaatccgga
     7381 gatcatttcc gccacgggta ataatagtca caaatatatt aatgataatc actatcacca
     7441 tatcgtcacg cctgaagcca tcagtctggc gttggaaaac cacgagttta aaccgtggat
     7501 ccaaccggtt ttctgcgcgc agactggggt actgacgggc tgtgaagtgc ttgtccgctg
     7561 ggaacatcca caaacgggaa tgatcccacc ggatcagttt attcctctgg cggagtcatc
     7621 cggtcttatt gtcataatga ctcgccagct gatgaaacag actgcggata ttctgatgcc
     7681 ggtaaaacat ttgctgccgg acaatttcca tattggtatc aacgtctcgg cgggctgttt
     7741 tttggctgcg ggatttgaaa aagagtgtct gaacctggtt aagaaattag gtaacgataa
     7801 aatcaagctg gttcttgagt tgacggaacg taatcctatt ccggtaacgc cagaagccag
     7861 agcgatattt gacagccttc atcagcacaa tattaccttt gcgctggatg actttggtac
     7921 gggttatgcg acctatcgtt acttgcaggc gttcccggtc gattttatta aaattgacaa
     7981 gtcgtttgtg caaatggcga gtgtcgacga aatctccggt catattgtgg acaatattgt
     8041 cgaactggcg cgtaagcctg gtttgagtat cgtggcggaa ggggtagaaa cccaggagca
     8101 ggcggattta atgatcggta aaggcgttca ctttttacag ggctatttgt actctccgcc
     8161 agtaccgggt aataaattta tctctgaatg ggtaatgaaa gcaggtggtt gatgtaaacc
     8221 gctattcaca gcgcatcggg aggttggcag cgattaattc tcccgatgca gtaaatcctg
     8281 atagatcccc gttaatatgc catttggcgc aaattgtttt ttaatccact gcgtcacctg
     8341 accggtggca gagtgctggg ttgcgagtaa catgcgtgaa tcctggcgcg ggttatggat
     8401 ttgtcgggta accagcaagg attgcgccat cgcctcacgg accatgtaat ccggcaaaaa
     8461 accaattcct tcacctaaaa tctggcactg acatttggtg ttgaaatcgg gcaccagaat
     8521 cgattcctgc ccatgcagca accagcctac ttttttatta atcgtatgcg cggtgtcctc
     8581 caccatgata ttagggtaga gacgcagctg gctttcggcg atcggctccg gcacaaaggc
     8641 taatggatga tccggagcga tggcaaaagc ccagcgaatc gcgccaatct ccgtgtaatc
     8701 aatgccgccg ccgtccagca gcgtgtcggg cgcgccgatg gcgatatttg cctgattatt
     8761 aataatcgca tcccagacgc cgttatacac ttcggtcgtc acggtaatct ggcaggtagg
     8821 aaactgcttt ttcagcacct gtaataagcg cgcggtgtgt ttgggcgtat aaagcagcag
     8881 attaatacaa atgcgcacac gcgcttctat gccctgcgaa atagtatcga tactgcgttt
     8941 aatggcgtga aaatcattca gcaggtcggt agctttgcgg aaaaaataac gcccggactc
     9001 cgttaactca atgctgcggg tactgcgagt gaacagcacc acatccagcc ccgtctccat
     9061 acgtttaatg gtgtagctaa tggcggaagt ggttaaaccc aactcttctg cggctttact
     9121 gaagctgcca aaacgtgcag cggtagtaaa tgccaacaga ttctcttcgg taaaaattga
     9181 gttcattaaa ccattcccgc cattatctaa tatgaatttc tccagccatg actgtagagg
     9241 tatcggttaa agatagataa tcatttttga ataactttta atacccgtcg cgtttagtcg
     9301 tcgcttcgct taatttttgt ccgccgtatt ctgaaattat ccccacgcag ttacccgcca
     9361 gaaaacccag taatgtcacc gtccagttca tcccgctggc aaaaaatagc gtcatgccga
     9421 gaaagccacc tggaataaaa gacagtaacc agaaacgtcc ttgccagacc actcccgccg
     9481 agaaaggcac ggtagtaatc acgctcgccc agaacccgga aatcccactc accgaggcca
     9541 gccaaccggc agccagcgcg gcgaaaaacg cccacaccat cccggaataa ttaaccgcca
     9601 ggcttctgat aaaccccgat ttaccgccgc ctgcggcata aaaactgcaa aaggcgacga
     9661 agccaatggt tcctaataat tcccatccct gagagcgccc gacatgtgaa gacactaact
     9721 gccaaagccc tgcacaaata ccgacggtga cacccgttgc cgtgagtccg ttcatcttgt
     9781 tcccctctgg ttagacatgg acgaaagcta ctcttacatg tgcagactat agagaaataa
     9841 aacctgattg agtgaaaggg tatcgggtca aagaaacaaa tattgcacta ccgcacacgg
     9901 cgaaaagatt gttgaacatc attcaacaaa aaggcgaaat acggcgaatt aaatttaagc
     9961 gggttctttt atcagattat cgttgctatt ctcaattcat cgaaaacagc cacaaaaacc
    10021 aggggaatgt gaatgactat aaaaaatcta cctgccgact atttattggc tgcgcaacag
    10081 ggggatatcg ataaagtaaa aacctgcctt gcgctgggtg tcgatattaa tacctgcgat
    10141 cgtcagggga aaacggcaat tacgctggca agtttatatc agcaatatgc ttgcgttcag
    10201 gcattaattg acgccggagc gaatattaat aaacaagacc tgacatgttt aaatcctttt
    10261 ttaattagct gtctgaacga tgatttaacg ctactacgaa ttattttacc ggctaaaccc
    10321 gatcttaatt gcgtaacccg ttttggcggt gtcggcctga cgcctgcctg tgaaaaaggc
    10381 catttaagta ttgtaaaaga gcttttggca aatacggaga ttaacgttaa ccagactaac
    10441 catgttggct ggacaccgtt gctggaagcg attgtgctta atgatggtgg gattaaacag
    10501 caggcgattg tccagttatt gctggaacac ggtgccagcc cgcatctgac cgataaatat
    10561 ggcaaaacgc cgctgaaact ggcgcgggaa cggggctttg aagagattgc gcagttgctg
    10621 atcgccgcag gtgcataaac cgggaggctt gttatcaaca caccagaaaa acggtgtgtg
    10681 tgggcgctaa ctgcggatgc tgattttctg gcgcagcggg ggcaaggaca ggttgaacag
    10741 gtctttgcca gagcggtaaa tatcgcactc ccggctcgcc agcagttgct gacgctgctt
    10801 tgtgaagagt acgacaatgc gcctaacagt tgtcggttgg cactcactca ctttaatggt
    10861 ctgttccggc atgatgataa ggttcagttt gacgatcaag gcattacgat tggtcaacat
    10921 catcatatag agatgagtca ttgccagcgt tggctgtccc caaccttgca aatgaccgct
    10981 gtgaattttc accttatcgc ctggcaacag tggtacgaca ttattcatca gcacctgggg
    11041 gaaaatgaaa ccctgtttaa ttatcgcggc gataatccgt tttatcaggc gttaaataaa
    11101 gaactacata ttaaacgacg ggcagttatt caggccgtaa acgataaaca aaatatcgcc
    11161 tcagcagtcg ccagtatgat ggggttaggg attggcctta cgccatcagc cgatgattat
    11221 ctgaccggac tggtacttat tttatttatt tccggacatc cggcggaaaa atacaaagag
    11281 gaattttatc tcggcctgca acgcggcaga aataatacca cgttattaag tgccatcacg
    11341 ctggaagccg cattacaaca acgctgccgg gaaaatattc atcgttttat tcacaacatt
    11401 atttatgaca tccctgggaa cgcaactcag gcaatagaaa aaattaaaca tattggctcc
    11461 agttccggct gcgacatgct gtatggcatg gccgatggtt gtgcgctgag tcaaacctac
    11521 ggagggaatt atgtcagtta aaatagtcat taaaccgaat acctattttg attctgtctc
    11581 gctgatgtct atctccacgc gtgcaaataa actcgacggc gtcgagcagg catttgtggc
    11641 gatggcgacc gaaatgaaca aaggcgtgtt gaagaattta ggactgctga cgccggagct
    11701 ggagcaggcg aaaaacggcg acctgatgat tgtcatcaat ggtaaatcgg gtgtggacaa
    11761 cgagcagttg ctggcggaga ttgaagaact gttcaacacc aaagcgcaaa gcggctcgca
    11821 cgaggcgcgt tacggcacta ttgccagcgc caaaaagcat atcccggaaa gtaacctggc
    11881 agtgatttcg gtcaacggtc tgtttgccgc tcgcgaagcg cgtcaggcgc tgcaaaatga
    11941 cctcaacgtg atgctgtttt ccgataatgt ctcagttgaa gatgaactgg cgctcaaaca
    12001 gttggcccat gaaaaagggc tgctgatgat ggggccagac tgcggcacgg cgattatcaa
    12061 cggcgcggcg ctctgctttg gtaacgccgt gcgtcgcggc aacatcggta ttgttggcgc
    12121 atctggcacc ggcagccagg aattgagcgt ccgtatccat gaatttggtg gcggcgtttc
    12181 acaactgatc ggcaccggcg ggcgcgacct gagcgagaaa atcggcggac tgatgatgct
    12241 cgatgccatc agcatgctgg aaaacgatcc gcaaactgaa atcattgtgc ttatctccaa
    12301 accgcctgcg cctgcggtgg cccgcaaagt gctggaacgc gcacgcgcct gccgcaagcc
    12361 agtggtggcc tgcttcctcg gtcgtggcga aacgccagtg gatgagcagg ggctacagtt
    12421 tgctcgcggc agcaaagagg cggcattgaa agcggtgatg ctctccggcg tgaaacaaga
    12481 acatctcgat ctgcatacgc ttgaccagcc gttgattgcg gatgtgcgcg cgcgtctgca
    12541 accgcagcag aaatacattc gtggtctgtt ctgcggcggt acgctgtgtg acgaaaccct
    12601 gttcgcggtg atggaaaaac atggcgatgt ctacagcaat atccagcccg atcctgaatt
    12661 ccgcctgcaa gatatcaacc gcagcatcaa acacaccttc ctcgactttg gcgatgatga
    12721 cttcaccaac ggcaagccgc atccaatgat tgacccgacc aaccgtatca gccgcttgat
    12781 cgaagaagcg cgcgatccag aagtggcggt gatcgtgatg gattttgtgc tcggatttgg
    12841 atcgcatgaa gatccggtcg gttccaccat cgaggcgatc aaagaagcga aagcgatcgc
    12901 cgctgccgaa ggacgcgagt tgatcattct cgcctatgtg ctgggcaccg atctcgatac
    12961 gccatcatta gaacaacaaa gccagatgct gcttgatgcc ggagtgattc tggcgagcag
    13021 cagcaccaat accggattgc tggcgcgtga atttatctgc aaaggggagg aagcctgatg
    13081 agccagtcac tgtttagcca accgttgaac gttattaacg tcggtatcgc catgtttagc
    13141 gatgatctga aaaagcagca tgtagaagtg acccaactcg actggacgcc gccggggcag
    13201 ggcaatatgc aggtggtgca ggcactggac aacattgccg attcgccgct ggcggacaaa
    13261 atcaccgccg ctaaccagca ggcgctggag cgcattatcc agtcgcatcc agtgctgatt
    13321 ggttttgatc aggcgattaa cgtggtgccg ggcatgacgg cgaaaaccat tcttcacgcc
    13381 gggccgccgg tcacctggga aaaaatgtgc ggcgcgatga aaggcgcggt caccggagcg
    13441 ctggtgtttg aaggactggc gaaagatctc gacgaggcgg ctgagctggc ggcttccggg
    13501 gagatcacct tctcgccgtg ccacgagcac gactgtgtgg gatcgatggc gggcgtcacc
    13561 tcggcctcga tgtttatgca catcgtgaaa aacaaaacct atggcaacat cgcttatacc
    13621 aacatgagcg agcagatggc gaagattttg cgtatgggcg ctaacgacca gagcgtgatc
    13681 gaccgcctca actggatgcg cgatgtgcag gggccaatgc tgcgtgacgc gatgaaaatt
    13741 atcggcgaga tcgatctgcg cttaatgctg gcgcaggcgc tgcatatggg cgatgaatgc
    13801 cataaccgca ataacgccgg gacgacgctg ctgattcagg cgctgacgcc ggggattatt
    13861 caggcgggtt attccgttga gcaacagcgc gaagtgtttg agtttgtcgc cagcagcgat
    13921 tacttctccg gcccaacgtg gatggcgatg tgtaaagcgg cgatggatgc ggcgcacggc
    13981 attgaataca gcaccgtggt gaccaccatg gcgcgtaacg gcgtcgagtt cggcctgcgg
    14041 gtcagcggcc tgccggggca atggtttacc ggcccggcgc agcaggtcat cggcccgatg
    14101 tttgccggtt ataagccgga agattcgggg ctggatatcg gcgacagcgc catcaccgaa
    14161 acctacggta tcggcggatt tgcgatggcg accgcgcccg cgatcgttgc gctggtgggc
    14221 ggcacggtgg aagaggctat cgatttctcc cgtcagatgc gtgaaatcac ccttggcgaa
    14281 aaccccaacg tcaccattcc gctgctcggt tttatgggcg tgccgtcggc aatcgacatc
    14341 acccgcgtgg gtagcagcgg cattctgccg gtgatcaaca ccgccattgc ccataaagat
    14401 gcgggcgtcg gcatgattgg cgcgggcatt gtgcatccac ccttcgcctg cttcgagaaa
    14461 gccattcttg gctggtgcga acgttacggc gtctgacctg caaagtacgc gttgtctctg
    14521 ccggatgcgg cgtgaacgcc ttatccggcc tacgagtggc acgagaattg taggcctgat
    14581 aagcgtagcg catcaggcag tctggcgttg gtcataaccc catcaccctc tgtcgcggac
    14641 ataacaacat gaaagaactt gtggtcgttg ccattggtgg caacagcatt atcaaagata
    14701 acgccagcca gtcgattgag catcaggcgg aggcggtgaa agccgtggcc gatacggtgc
    14761 tggaaatgct ggcttccgat tacgacattg tgctgaccca cggcaacggg ccgcaggtcg
    14821 ggctggattt acgccgtgcg gagattgccc acgagcgcga agggctaccc ttaacgccgc
    14881 tggcgaactg tgtagcggat acgcaaggcg gcatcggcta tctgatccag caggcgctga
    14941 acaaccgact ggcgcgtcac ggcgagaaaa aagccgtcac cgtggtgact caggtggaag
    15001 tggataaaaa cgatccgggt tttgcccatc ccaccaagcc catcggcgca ttctttagtg
    15061 aaagccagcg tgacgaacta caaaaggcaa accctgactg gcgttttgtt gaagatgccg
    15121 gacggggcta tcgccgcgtg gtcgcctcgc cggaaccgaa acgtattgtc gaagcacctg
    15181 ccattaaggc gctgatccaa caaggctttg tggtgattgg cgcgggcggc ggtggaattc
    15241 ctgtggtgcg caccgaagcg ggagattacc aaagcgtgga cgcggttatc gacaaagatc
    15301 tctccaccgc gctactggcc cgcgaaattc acgccgacat tctggtgatc accaccggcg
    15361 tcgaaaaagt ctgtatccac tttggcaagc cgcagcaaca ggcgctcgat cgcgtagata
    15421 ttgccaccat gacccgctat atgcaggaag ggcatttccc acctggcagc atgttgccaa
    15481 aaatcatcgc cagcctgacg ttcctggaac agggcggcaa agaagtgatt atcaccacgc
    15541 cggaatgcct gcctgcggcg ctacgcggcg aaacgggcac ccatattatt aaaacctaag
    15601 gacgtaagat gaaagaaaac aatagccgcc gtgaatttct gagccagagc ggtaagatgg
    15661 tcaccgccgc cgcgctgttt ggtacctcag tgccgctcgc ccatgcgggg gtagctggca
    15721 ccctaaactg cgaagcgaac aacaccatga aaatcactga cccgcattac tatctcgata
    15781 acgtgctgct ggaaaccggt tttgactacg aaaatggcgt ggcggtacag acccgcacgg
    15841 cgcgccagac cgtggagatt caggacggta aaattgttgc cctgcgcgag aacaagcagc
    15901 atccggacgc cacgctgccg cactatgacg ctggcggtaa gctgatgctg cccaccaccc
    15961 gcgatatgca tattcatctc gacaaaacct tctacggcgg gccgtggcgc tcgctcaatc
    16021 gtccggcagg caccactatc caggacatga tcaaactcga gcagaaaatg ctgccggaac
    16081 tgcaaccgta cacgcaggaa cgggcggaaa aactgatcga tttattgcag tcgaaaggca
    16141 ccactattgc ccgcagccac tgcaatatcg aaccggtttc cggcctgaaa aatctgcaaa
    16201 atttgcaggc ggtgctggcg cgacgtcaga cgggcttcga gtgtgagatc gtcgccttcc
    16261 cgcagcacgg tttgctgctg tcgaaatcgg aatccttaat gcgcgaagcg atgcaggcgg
    16321 gggcgcatta cgtcggcggg ctggacccga ccagtgttga tggcgcgatg gaaaaatccc
    16381 tcgacaccat gttccagatt gcgctggact atgacaaagg cgtcgatatt cacctgcacg
    16441 aaaccacgcc ggcaggcgtg gcagccatca attatatggt tgaaacggtg gagaaaacgc
    16501 cgcaactgaa gggcaagctg accatcagcc acgcctttgc gctggcaacg ctcaacgaac
    16561 aacaggtaga taaactggcg caccggatgg tggcgcagca aatttctatc gcctcgacgg
    16621 tgccgattgg cacgttgcat atgccgctca aacagctaca cgacaaaggc gtaaaagtga
    16681 tgaccggcac cgacagcgtt atcgaccact ggtcacccta cggcctgggc gacatgctgg
    16741 aaaaagccaa tctgtacgcg cagctctata ttcgccctaa cgaacagaat ttatcccgct
    16801 cgctgttttt agccaccggt gatgtattgc cgctaaatga aaaaggcgag cgcgtgtggc
    16861 caaaagcgca ggatgacgcc agttttgtgc tggtggacgc ctcctgttcc gccgaggcgg
    16921 tggcgcgtat ttcgccgaga acggcgacgt tccataaagg gcaactggtg tgggggagtg
    16981 tggcaggttg atacgaactt gatatgtctg caacggtgaa cagtaagaga tttaagcccc
    17041 agggagtggg gcggcctgag tatgagtgtg tcggcagaaa tggacattat ctttgaagaa
    17101 tatgccctta ttgacgttgt gtagataact atttgtctac gtgaaaatat catcagtttt
    17161 gccgattttt gtcttttacg gatagcaaat atcacactta taggcgctcg ccttatgggg
    17221 aacgttgctg ctctgtttca ttcaggctcg cggcatgtta gggctggatc gggtggttta
    17281 aatattggct atattcaatg gacgcgtttt gccgcgatga catatcaggc atcgtcaaat
    17341 acacatagct aatcaggagt aaacacaatg aagatcaaag ctgttggtgc atattccgct
    17401 aaacaaccgc ttgaaccgat ggatatcacc cggcgtgaac cgggaccgca tgatgtcaaa
    17461 atcgaaatcg cttactgtgg cgtctgccat tccgatctcc accaggtccg ttcggagtgg
    17521 gcggggacgg tttacccctg cgtgccgggt catgaaattg tggggcgcgt ggtagccgtt
    17581 ggtgatcagg tagaaaaaca tgcgccgggc gatctggtcg gtgtcggctg cattgtcgac
    17641 agttgtaaac attgcgaaga gtgtgaagac gggctggaaa actactgtga tcacatgacc
    17701 ggaacctata actcgccgac accggatgaa ccgggccata ctctgggcgg ctactcacaa
    17761 cagatcgtcg ttcatgagcg atatgttctg cgtattcgtc acccgcaaga gcagctggcg
    17821 gcggtggctc ctttattgtg tgcaggaatc accacgtact cgccgctacg ccactggcag
    17881 gccgggccgg gtaaaaaagt gggcgtggtc ggcatcggcg gtctgggaca tatggggatt
    17941 aaactggccc acgcgatggg ggcgcatgtg gtggcattta ccacttctga gtcaaaacgc
    18001 gaagcggcaa aagccctggg ggccgatgaa gttgttaact cacgcaatgc cgatgagatg
    18061 gtggctcatg tgaagagttt cgatttcatt ttgaatacag tagctgcgcc acataatctc
    18121 gacgatttta ccaccttgct gaagcgcgat ggcaccataa cgctggttgg tgcgcctgcg
    18181 acaccgcata aatcgccgga agttttcaac ctgatcatga aacgccgcgc gatagctggt
    18241 tctatgattg gcggcattcc agaaactcag gagatgctcg atttttgcgc cgaacatggc
    18301 atcgtggctg atatagagat gattcgggcc gatcaaatta atgaagccta tgagcgaatg
    18361 ctgcgtggtg atgtgaaata tcgttttgtt atcgataatc gcacactaac agactgaaaa
    18421 aattaataaa tatcctgtgg tttaacatat taacttcgct ctccttaact ttttagttaa
    18481 ggagagcgaa ataatatcaa agtagtggta aacctatagc gtaaattcag attgttaaat
    18541 taactctact actttacacg ttagttatga gcgatttctg atagtgtctg gtttaatcag
    18601 agctttatta tctgcgacgt ttatttttat ttaagagagc cacctgcaag ttgttattcc
    18661 accagagagt gaatcgcaaa gcaggtcatc atttcatctg tatatctgta tgccaaatgg
    18721 cattccccct cctttttcgc cgatattttg ttgtgatcga cgaagtttcg cttaaaaaag
    18781 caccagcagt ggtttcgcag ctatatggtg tataaaaaat gttctcatac agatgttttg
    18841 tgaatgtgtg ggttgacatt catatgaaaa aaatcataat tccatcatgt ttgtgtgggg
    18901 gcttttctgt atcttacgca tcgcactcaa gcctgacaga aaatatgctg taaggctcat
    18961 atcaaaaacc gccattagct catcaggaag agcagacgac aaccaggatt gttgtatggt
    19021 acggggttcg aggcctcgat ggcggtccaa cttttcaaaa ctcttctgaa tcagatcccc
    19081 ttattatcat ccctgcaaag tggaacattg ccattgtttg gctattcagg acgggtcaga
    19141 ccaatgaaaa agcccacaca ggggagagta ggctgaaatg ggaagctaaa gactcaagta
    19201 aacttatcgg aaataaggac cacgcattac gggggctatc atcgccgatg gttgggattc
    19261 ggcaaatcag gttgattacg tagagagtaa attatctgct caccgctgcg tcaccccttc
    19321 gtaaatcagg cgtagcgcga atacaccaat aattgcacca ataacccgac tggcaacgcg
    19381 ttgcatacgc ccataagcac gacgcacagc gggcaaagaa aacgcctggc ttagaaagac
    19441 tcgccagata attgatgtga gcacaatccc cgcccaggcc attaatcgtg cccatgttgg
    19501 t
//
LOCUS       DAHHTG010000081        19270 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.081, whole genome shotgun sequence.
ACCESSION   DAHHTG010000081 DAHHTG010000000
VERSION     DAHHTG010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19270)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19270)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19270
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.081"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            <1..901
                     /locus_tag="NBI74_002969"
     CDS             <1..901
                     /locus_tag="NBI74_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCD1933262.1"
                     /translation="ANLYDLKKFDLNLLVIFECIYQHLSISKAAESLYITPSAVSQSL
                     QRLRAQFNDPLFIRSGKGIAPTTTGLNLHHHLEKNLRGLEQTINIVNKSELKKNFIIY
                     GPQLISCTNNNMLIRCLRQDTSIDIECHDILLSAENAEELLVQRKTDLAITLQPVISR
                     SVICMPLHTIRNSLICSNKHPRITDTSNYEQIIAEEFTLLISKSAGIDEIQMDIDERF
                     INRKVSFRSSSLLTIINSISVTDLLGIVPTELYDLHRDFLKLKEIKLEQPLPAVKLYI
                     SYNKASLNNLVFSRFIDRLNDSF"
     gene            1048..2268
                     /locus_tag="NBI74_002970"
     CDS             1048..2268
                     /locus_tag="NBI74_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HCD1933263.1"
                     /translation="MSIYKIPLPLNILEAAKERITWTLNTLPRICVSFSGGKDSGLML
                     HLTAEIARQMGKKICVLFIDWEAQFSCTINYVQSLREFYTDVIEEFYWVALPLTTQNS
                     LSQYQPEWQCWEPDVEWVRQPPQDAITDPDFFSFYQPGMTFEQFVREFAEWFSQKRPA
                     AMMIGIRADESYNRFVAIASLNKQRFADDKPWTTAAPGGHSWYIYPIYDWKVADIWTW
                     YANHQQLCNPLYNIMYQAGVPLRHMRICEPFGPEQRQGLWLYHVIEPDRWAAMCARVS
                     GVKSGGIYAGHDNHFYGHRKILKPEHLDWQEYALLLLNSMPEKTAEHYRNKIAIYLHW
                     YQKKSITVPQTQQGDIGAKDIPSWRRICKVLLNNDYWCRALSFSPTKAKNYQRYNERI
                     KGKRKEWGILCNND"
     gene            2241..2870
                     /locus_tag="NBI74_002971"
     CDS             2241..2870
                     /locus_tag="NBI74_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB-like nuclease domain-containing protein"
                     /protein_id="HCD1933264.1"
                     /translation="MGDTMQQRLTQDLTQFLASLPEDDRINAINEIRMAIHQVSPFRE
                     EPVDCVLWVKNSQLMPNDYNPNNVAPPEKKLLKKSIEIDGFTQPIVVTHTDKNALEIV
                     DGFHRHEIGKGSSSLKLRLKGYLPVTCLEGTRNQRIAATIRHNRARGRHQITAMSEIV
                     RELSQLGWDDNKIGKELGMDSDEVLRLKQINGLQELFADRQFSRAWTVK"
     gene            complement(2871..4031)
                     /gene="ybdL"
                     /locus_tag="NBI74_002972"
     CDS             complement(2871..4031)
                     /gene="ybdL"
                     /locus_tag="NBI74_002972"
                     /EC_number="2.6.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine-oxo-acid transaminase"
                     /protein_id="HCD1933265.1"
                     /translation="MTNNPLIPQSKLPQLGTTIFTQMSALAQQHQAINLSQGFPDFDG
                     PRYLQERLAYHVAQGANQYAPMTGVQALREAIAQKTERLYGYQPDVDSNITVTAGATE
                     ALYAAITALVRNGDEVICFDPSYDSYAPAIALSGGIVKRIALQPPHFRVDWQEFAALL
                     SERTRLVILNTPHNPSATVWQQTDFAALWQAIAGHEIFVISDEVYEHINFSQQGHASV
                     LAHPQLRERAVAVSSFGKTYHMTGWKVGYCVAPAPISAEIRKVHQYLTFSVNTPAQLA
                     LADMLRAEPEHYLALPDFYRQKRDILVNALNESRLEILPCEGTYFLLVDYSAVSTMDD
                     VEFCQWLTREHGVAAIPLSVFCADPFPHKLIRLCFAKKESTLLAAAERLRQL"
     gene            4140..5228
                     /gene="hcxA"
                     /locus_tag="NBI74_002973"
     CDS             4140..5228
                     /gene="hcxA"
                     /locus_tag="NBI74_002973"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcxA"
                     /protein_id="HCD1933266.1"
                     /translation="MPHNPIRVVVGPANYFSHPGSFNHLHDFFTDEQLSRAVWIYGER
                     AIAAAQTKLPPAFELPGVKHILFRGHCSESDVQQLAAESGDDRSVVIGVGGGALLDTA
                     KALARRLGLPFVAVPTIAATCAAWTPLSVWYNDAGQALHYEIFDDANFMVLLEPEIIL
                     NAPQEYLLAGIGDTLAKWYEAVVLAPQPETLPLTVRLGINNAQAIRDVLLNSTEQALA
                     DQQNQQLTQSFCDVVDAIIAGGGMVGGLGDRFTRVAAAHAVHNGLTVLPQTEKFLHGT
                     KVAYGILVQSALLGQDDVLAQLTEAYQRFHLPTTLAELEVDINNQVEIDKMIAHTLRP
                     VESIHYLPVTLTPDTLRAAFEKVESFKA"
     gene            complement(5238..5435)
                     /locus_tag="NBI74_002974"
     CDS             complement(5238..5435)
                     /locus_tag="NBI74_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HCD1933267.1"
                     /translation="MFDSLAKAGKYLGQAAKLMIGMPDYDNYVEHMRVNHPDQTPMTY
                     EEFFRERQDARYGGKGGARCC"
     gene            complement(5548..7653)
                     /gene="cstA"
                     /locus_tag="NBI74_002975"
     CDS             complement(5548..7653)
                     /gene="cstA"
                     /locus_tag="NBI74_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation protein CstA"
                     /protein_id="HCD1933268.1"
                     /translation="MNKSGKYLVWTVLAVMGAFALGYIALNRGEQINALWIVVASVCI
                     YLIAYRFYGLYIAKNVLAVDPTRMTPAVRHNDGLDYVPTDKKVLFGHHFAAIAGAGPL
                     VGPVLAAQMGYLPGMIWLLAGVVLAGAVQDFMVLFVSTRRDGRSLGELVKEEMGPTAG
                     VIALVACFMIMVIILAVLAMIVVKALTHSPWGTYTVAFTIPLALFMGIYLRYLRPGRI
                     GEVSVIGLVFLIFAIISGGWVAESPTWAPYFDFTGVQLTWMLVGYGFVAAVLPVWLLL
                     APRDYLSTFLKIGTIVGLAVGILIMRPTLTMPALTKFVDGTGPVWTGNLFPFLFITIA
                     CGAVSGFHALISSGTTPKMLANEGQACFIGYGGMLMESFVAIMALVSACIIDPGVYFA
                     MNSPMAVLAPAGTADVVASAAQVVSSWGFAITPDTLNQIASEVGEQSIISRAGGAPTL
                     AVGMAYILHGALGGMMDVAFWYHFAILFEALFILTAVDAGTRAARFMLQDLLGVVSPG
                     LKRTDSLPANLLATALCVLAWGYFLHQGEVDPLGGINTLWPLFGIANQMLAGMALMLC
                     AVVLFKMKRQRYAWVALVPTAWLLICTLTAGWQKAFSPDAKVGFLAIANKFQAMIDSG
                     NIPSQYTESQLAQLVFNNRLDAGLTIFFMVVVVVLALFSIKTALAALKEPKPTAKETP
                     YEPMPENVEEIVAQAKGAH"
     gene            complement(7834..8247)
                     /gene="entH"
                     /locus_tag="NBI74_002976"
     CDS             complement(7834..8247)
                     /gene="entH"
                     /locus_tag="NBI74_002976"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proofreading thioesterase EntH"
                     /protein_id="HCD1933269.1"
                     /translation="MIWKRHLTLDELNATSDNTMVAHLGIVYTRLGDDVLEAEMPVDT
                     RTHQPFGLLHGGASAALAETLGSMAGFMMTRDGQCVVGTELNATHHRPVSEGKVRGVC
                     QPLHLGRQNQSWEIVVFDEQGRRCCTCRLGTAVLG"
     gene            complement(8250..8996)
                     /gene="entA"
                     /locus_tag="NBI74_002977"
     CDS             complement(8250..8996)
                     /gene="entA"
                     /locus_tag="NBI74_002977"
                     /EC_number="1.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase
                     EntA"
                     /protein_id="HCD1933270.1"
                     /translation="MDFSGKNVWVTGAGKGIGYATALAFVEAGAKVTGFDQAFSQEQY
                     PFATEVMDVADAAQVAQVCQRLLAETERLDVLVNAAGILRMGATDQLSKEDWQQTFAV
                     NVGGAFNLFQQTMNQFRRQRGGAIVTVASDAAHTPRIGMSAYGASKAALKSLALSVGL
                     ELAGSGVRCNVVSPGSTDTDMQRTLWVSDDAEEQRIRGFGEQFKLGIPLGKIACPQEI
                     ANTILFLASDLASHITLQDIVVDGGSTLGA"
     gene            complement(8996..9853)
                     /gene="entB"
                     /locus_tag="NBI74_002978"
     CDS             complement(8996..9853)
                     /gene="entB"
                     /locus_tag="NBI74_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin biosynthesis bifunctional
                     isochorismatase/aryl carrier protein EntB"
                     /protein_id="HCD1933271.1"
                     /translation="MAIPKLQAYALPESHDIPQNKVDWAFEPQRAALLIHDMQDYFVS
                     FWGENCPMMEQVIANIAALRDYCKQHNIPVYYTAQPKEQSDEDRALLNDMWGPGLTRS
                     PEQQKVVDRLTPDADDTVLVKWRYSAFHRSPLEQMLKESGRNQLIITGVYAHIGCMTT
                     ATDAFMRDIKPFMVADALADFSRDEHLMSLKYVAGRSGRVVMTEELLPAPIPASKAAL
                     REVILPLLDESDEPFDDDNLIDYGLDSVRMMALAARWRKVHGDIDFVMLAKNPTIDAW
                     WKLLSREVK"
     gene            complement(9867..11477)
                     /gene="entE"
                     /locus_tag="NBI74_002979"
     CDS             complement(9867..11477)
                     /gene="entE"
                     /locus_tag="NBI74_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2,3-dihydroxybenzoyl)adenylate synthase EntE"
                     /protein_id="HCD1933272.1"
                     /translation="MSIPFTRWPEEFARRYREKGYWQDLPLTDILTRHATSDSIAVID
                     GEWQLSYRELNQAADNLACSLRRQGIKPGETALVQLGNVAELYITFFALLKLGVAPVL
                     ALFSHQRSELNAYASQIEPALLIADRQHALFSGDDFLNTFVAEHSSIRVVQLLNDSGE
                     HNLQDAINHPAEDFTATPSPADEVAYFQLSGGTTGTPKLIPRTHNDYYYSVRRSVEIC
                     QFTQQTRYLCAIPAAHNYAMSSPGSLGVFLAGGTVVLAADPSATLCFPLIEKHQVNVT
                     ALVPPAVSLWLQALAEGESRAQLASLKLLQVGGARLSATLAARIPAEIGCQLQQVFGM
                     AEGLVNYTRLDDSAEKIIHTQGYPMCPDDEVWVADAEGNPLPQGEVGRLMTRGPYTFR
                     GYYKSPQHNASAFDANGFYCSGDLISIDPEGYITVQGREKDQINRGGEKIAAEEIENL
                     LLRHPAVIYAALVSMEDELMGEKSCAYLVVKEPLRAVQVRRFLREQGIAEFKLPDRVE
                     CVDSLPLTAVGKVDKKQLRQWLASRTSA"
     gene            complement(11487..12662)
                     /gene="entC"
                     /locus_tag="NBI74_002980"
     CDS             complement(11487..12662)
                     /gene="entC"
                     /locus_tag="NBI74_002980"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase EntC"
                     /protein_id="HCD1933273.1"
                     /translation="MDTSLAEEVQQTMATLAPNRFFFMSPYRSFTTSGCFARFDEPAV
                     NGDSPDSPFQQKLAALFADAKAQGIKNPVMVGAIPFDPRQPSSLYIPESWQSFSRQEK
                     QASARRFTRSQSLNVVERQAIPEQTTFEQMVARAAALTATPQVDKVVLSRLIDITTDA
                     AIDSGVLLERLIAQNPVSYNFHVPLADGGVLLGASPELLLRKDGERFSSIPLAGSARR
                     QPDEVLDREAGNRLLASEKDRHEHELVTQAMKEVLRKRSSELHVPSSPQLITTPTLWH
                     LATPFEGKANSQENALTLACLLHPTPALSGFPHQAATQVIAELEPFDRELFGGIVGWC
                     DSEGNGEWVVTIRCAKLRENQVRLFAGAGIVPASSPLGEWRETGVKLSTMLNVFGLH"
     gene            12851..13807
                     /gene="fepB"
                     /locus_tag="NBI74_002981"
     CDS             12851..13807
                     /gene="fepB"
                     /locus_tag="NBI74_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe2+-enterobactin ABC transporter
                     substrate-binding protein"
                     /protein_id="HCD1933274.1"
                     /translation="MRLAPLYRNALLLTGLLLSGIAAVQAADWPRQITDSRGTHTLES
                     QPQRIVSTSVTLTGSLLAIDAPVIASGATTPNNRVADDQGFLRQWSKVAKERKLQRLY
                     IGEPSAEAVAAQMPDLILISATGGDSALALYDQLSTIAPTLIINYDDKSWQSLLTQLG
                     EITGHEKQAAERIAQFDKQLAAAKEQIKLPPQPVTAIVYTAAAHSANLWTPESAQGQM
                     LEQLGFTLAKLPAGLNASQSQGKRHDIIQLGGENLAAGLNGESLFLFAGDQKDADAIY
                     ANPLLAHLPAVQNKQVYALGTETFRLDYYSAMQVLDRLKALF"
     gene            complement(13811..15061)
                     /gene="entS"
                     /locus_tag="NBI74_002982"
     CDS             complement(13811..15061)
                     /gene="entS"
                     /locus_tag="NBI74_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin transporter EntS"
                     /protein_id="HCD1933275.1"
                     /translation="MNKQSWLLNLSLLKTHPAFRAVFLARFISIVSLGLLGVAVPVQI
                     QIMTHSTWQVGLSVTLTGGAMFVGLMVGGVLADRYERKKVILLARGTCGIGFIGLCLN
                     ALLPEPSLLAIYLLGLWDGFFASLGVTALLAATPALVGRENLMQAGAITMLTVRLGSV
                     ISPMIGGLLLATGGVAWNYGLAAAGTFITLLPLLSLPALPPPPQPREHPLKSLLAGFR
                     FLLASPLVGGIALLGGLLTMASAVRVLYPALADNWQMSAAQIGFLYAAIPLGAAIGAL
                     TSGKLAHSVRPGLLMLLSTLGAFLAIGLFGLMPMWILGVVCLALFGWLSAVSSLLQYT
                     MLQTQTPEAMLGRINGLWTAQNVTGDAIGAALLGGLGAMMTPVASASASGFGLLIIGV
                     LLLLVLVELRRFRQTPPQVTASDS"
     gene            15172..16176
                     /gene="fepD"
                     /locus_tag="NBI74_002983"
     CDS             15172..16176
                     /gene="fepD"
                     /locus_tag="NBI74_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-siderophore ABC transporter permease"
                     /protein_id="HCD1933276.1"
                     /translation="MSGSVAVTRAIAVPGLLLLLIIATALSLLIGAKSLPASVVLEAL
                     SGTCQSADCTIVLDARLPRTLAGLLAGGALGLAGALMQTLTRNPLADPGLLGVNAGAS
                     FAIVLGAALFGYSSAQEQLMMAFAGALVASLIVAFTGSQGGGQLSPVRLTLAGVALAA
                     VLEGLTSGIALLNPDVYDQLRFWQAGSLDIRNLHTLKVVLIPVLTAGATALLLSRALN
                     SLSLGSDTATALGSRVARTQLIGLLAITVLCGSATAVVGPIAFIGLMMPHMARWLVGA
                     DHRWSLPVTLLATPALLLFADIIGRVIVPGELRVSVVSAFIGAPVLIFLVRRKTRGGA
                     "
     gene            16173..17165
                     /gene="fepG"
                     /locus_tag="NBI74_002984"
     CDS             16173..17165
                     /gene="fepG"
                     /locus_tag="NBI74_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter permease"
                     /protein_id="HCD1933277.1"
                     /translation="MIYVSRRLIITCLLLLIACVMAGVWGLRSGAVTLETSQVFAALM
                     GDAPRSMTMVVTEWRLPRVLMALLIGAALGVSGAIFQSLMRNPLGSPDVMGFNTGAWS
                     GVLVAMVLFGQDLTAIALAAMAGGIVTSLLVWLLAWRNGIDTFRLIIIGIGVRAMLVA
                     FNTWLLLKASLETALTAGLWNAGSLNGLTWAKTSPSAPIIILMLIAAALLVRRMRLLE
                     MGDDTACALGVSVERSRLLMMLVAVVLTAAATALAGPISFIALVAPHIARRISGTARW
                     GLTQAALCGALLLLAADLCAQQLFMPYQLPVGVVTVSLGGIYLIVLLIQESRKK"
     gene            17162..17977
                     /gene="fepC"
                     /locus_tag="NBI74_002985"
     CDS             17162..17977
                     /gene="fepC"
                     /locus_tag="NBI74_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001601847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter ATP-binding
                     protein"
                     /protein_id="HCD1933278.1"
                     /translation="MTESVARLRGEQLTLGYGKYTVAENLTVEIPDGHFTAIIGPNGC
                     GKSTLLRTLSRLMTPAHGHVWLDGEHIQHYASKEVARRIGLLAQNATTPGDITVQELV
                     ARGRYPHQPLFTRWRKEDEDAVTKAMQATGITHLADQSVDTLSGGQRQRAWIAMVLAQ
                     ETAIMLLDEPTTWLDISHQIDLLELLSELNREKGYTLAAVLHDLNQACRYASHLIALR
                     EGKIVAQGAPKEIVTAELIERIYGLRCMIIDDPVAGTPLVVPLGRTAPSTAKI"
     gene            complement(17974..19107)
                     /gene="fepE"
                     /locus_tag="NBI74_002986"
     CDS             complement(17974..19107)
                     /gene="fepE"
                     /locus_tag="NBI74_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen length regulator"
                     /protein_id="HCD1933279.1"
                     /translation="MSSLNIKQGSEAHFPEYPLASPSNNEIDLLNLIEVLWRAKKTVM
                     AVIFAFACTGLLISFILPQKWTSAAVVTPPEPVQWQELEKTFTKLRVLDLDIKIDRTE
                     VFNLFIKKFQSVSLLEEYLRSSPYVMDQLKEAKIDELDLHRAIVALSEKMKAVDDNAS
                     KKKDEPSLYTSWTLSFTAPTSEEAQTVLSGYIDYISALVVKESIENVRNKLEIKTQFE
                     KEKLAQDRIKTKNQLDANIQRLNYSLDIANAAGIKKPVYSNGQAVKDDPDFSISLGAD
                     GIGRKLEIEKAVTDVAELNGELRNRQYLVEQLTKANVDDVKFIPFKYQLSPSLPVKKD
                     GPGKAIIVILSALIGGMVACGGVLLRHAMASRKQDAMMADHLV"
BASE COUNT         4680 a         5379 c         4809 g         4402 t
ORIGIN      
        1 ggctaatctt tatgatctaa aaaaatttga cctcaattta ctggttatat ttgaatgtat
       61 ttatcaacat ctcagcatta gtaaagcggc agaatcatta tatataacac cctctgctgt
      121 tagtcagtcg ttacaacgtt tacgtgcgca atttaatgac ccgttattca ttcgctcagg
      181 gaaaggtatc gcgccgacta caacagggtt aaacctgcat caccatcttg agaaaaatct
      241 cagaggcctt gaacaaacga tcaatatcgt taataagtct gaactaaaga aaaattttat
      301 tatctacggt cctcagctta tttcttgtac taataacaac atgctaatcc gctgtcttag
      361 acaagatact tcaatcgata tagaatgcca tgacatactg ctttcagctg aaaatgccga
      421 agaactgttg gttcaacgca aaactgacct ggcaattacg ctgcaaccag taattagtcg
      481 ctctgtcatc tgcatgccgt tacacactat tcgcaattcc cttatatgta gcaacaaaca
      541 tcctcgtatt actgatacca gtaactatga acaaattatc gccgaagaat tcaccctgtt
      601 gatatctaaa tcagcaggga ttgacgaaat tcaaatggac atagatgaac gttttataaa
      661 ccgtaaagtt tcatttcgca gttcttcttt gttgactata attaatagca tttctgttac
      721 tgacttatta ggcattgtgc ccaccgaact gtacgattta catcgtgact ttttgaagtt
      781 aaaagaaata aaactggaac aacctcttcc tgcggttaaa ctttatattt cctataacaa
      841 ggcttcgttg aacaaccttg tcttctctcg attcatagat cgtttaaatg atagcttctg
      901 attcattacc actgacaaat aatatattac gcatcaatta attcattgtg tgaatataaa
      961 ataatgtgct ctatcttgct tcgtttatcc tataattagc ggattcatct cacgagtcac
     1021 cccgtaacgt tcagagagcg tttttagatg tctatttata aaatcccgct tccactgaat
     1081 attctggaag ctgcaaaaga acgtattacc tggacactta ataccctacc ccgtatctgt
     1141 gtttcttttt cgggtggtaa agattccgga ttaatgctgc atctgacagc tgaaattgcc
     1201 cgacaaatgg gcaaaaaaat ctgcgttttg tttatcgact gggaagcgca gttctcatgc
     1261 actattaact atgttcagtc attacgtgag ttttacaccg atgtcatcga agagttttac
     1321 tgggttgcgc tcccgcttac gacgcaaaat tccctttctc aataccaacc cgaatggcag
     1381 tgctgggaac ctgatgtcga atgggtgcgt cagcccccgc aagatgcgat aaccgatcct
     1441 gactttttct ccttttacca gcctggcatg accttcgaac aatttgtgcg tgagtttgct
     1501 gaatggtttt cacaaaaacg tccggcggcg atgatgatcg gcatccgtgc agatgagtcc
     1561 tacaaccgtt ttgtcgccat cgccagttta aataaacaac gttttgccga tgataagccc
     1621 tggaccaccg ccgcaccagg cggtcatagc tggtacattt accccatcta cgactggaaa
     1681 gtggctgata tctggacctg gtatgctaac catcaacagc tctgtaaccc actgtataat
     1741 ataatgtatc aggcaggcgt ccctctgcgt catatgcgaa tttgcgaacc ttttggcccg
     1801 gagcaacggc aaggattatg gctctatcac gttatcgaac cggatcgctg ggctgctatg
     1861 tgcgcacgag tcagcggggt aaaaagtggc ggcatttacg ccggacatga taatcatttt
     1921 tatggtcatc ggaaaatcct caagccagaa catttagact ggcaagaata tgcattattg
     1981 ctgctcaata gcatgccgga aaaaacagcg gagcattacc gcaataaaat cgccatttat
     2041 ttgcactggt atcagaaaaa aagcatcacg gttccacaaa ctcagcaagg ggacattggg
     2101 gcgaaagata tcccctcctg gcggcgaata tgcaaagttt tactcaataa cgattactgg
     2161 tgtcgggcat tatcatttag ccctacgaaa gcgaagaact atcagcgtta taacgaacgg
     2221 ataaaaggaa aacgtaagga atgggggata ctatgcaaca acgattaacg caggatttaa
     2281 cccaatttct cgccagcttg ccggaagatg atcgcatcaa cgcgattaat gaaatccgca
     2341 tggcgatcca tcaggttagc ccctttcgtg aagagccagt ggattgcgtt ctgtgggtta
     2401 aaaacagcca acttatgcct aatgattaca acccaaacaa cgtggcacca cccgaaaaaa
     2461 aactgctgaa aaaatccatt gaaatagatg gttttaccca acccattgtg gtcacgcata
     2521 cggataaaaa tgccctggaa atcgtggatg gttttcaccg ccatgaaatt ggtaaaggct
     2581 catcatcgtt aaaattacgc ttaaaaggtt atttgccggt gacctgtctg gaaggtacgc
     2641 gtaatcaacg catcgccgcg actattcgtc ataatcgcgc tcgtggacgt caccaaatca
     2701 ctgccatgtc agaaatcgtc cgagaactca gccagttggg atgggacgat aataaaattg
     2761 gcaaagagct gggaatggac agcgacgaag tgttacgcct gaagcaaatt aacggcctgc
     2821 aagagttgtt tgccgaccgt caattttccc gcgcctggac ggttaaataa ctacagctgg
     2881 cgcaggcgtt ctgctgctgc cagcaacgtc gattccttct tggcaaaaca gagacgaatc
     2941 agtttatgtg ggaagggatc ggcgcaaaac accgacagcg gaatcgccgc tacgccgtgc
     3001 tcccgcgtca gccactggca aaactcaaca tcatccatcg tagatacggc gctgtaatcc
     3061 accagcaaaa agtatgtacc ttcgcacggt aaaatctcca gccgactttc atttaaggca
     3121 ttcaccagaa tatcgcgctt ctggcgataa aagtccggta acgcaagata atgctcaggt
     3181 tctgcacgta gcatatcggc aagcgccagt tgtgccgggg tattcaccga aaaggtcaga
     3241 tactgatgca ctttgcggat ctcggcgctg atgggcgcag gcgcaacgca ataacccact
     3301 ttccagccgg tcatatgata ggtcttgcca aacgatgaca ccgccaccgc tcgctcacgc
     3361 aattgcggat gcgccagcac gctggcatgg ccctgttgtg aaaaattgat gtgctcgtag
     3421 acttcatcgc taatgacaaa aatctcgtgc ccggcgatcg cctgccacag agcggcgaaa
     3481 tcagtctgct gccagacagt tgcactgggg ttatgcgggg tgttgaggat caccagtcgg
     3541 gtgcgctcgc ttaacaatgc ggcaaattcc tgccagtcaa cgcgaaaatg cggcggttgc
     3601 agtgcaatac gtttcactat tccccccgaa agtgcgatgg cgggggcgta actgtcatag
     3661 ctgggatcaa aacaaatcac ttcgtcgcca ttgcgcacca atgcggttat cgccgcgtat
     3721 aacgcctccg tcgcccctgc cgtcacggtg atattgctgt cgacatccgg ttgatagcca
     3781 tacagacgtt ccgttttctg agcaatcgcc tcgcgcaagg cctgcacgcc ggtcatgggt
     3841 gcgtattggt ttgccccctg ggcaacgtgg tacgccagcc gctcctgtaa atagcgcgga
     3901 ccatcaaaat caggaaagcc ttgcgaaagg ttaatcgcct ggtgttgctg cgccagcgcg
     3961 ctcatctggg tgaaaatagt ggtgccaagt tgtggaagtt tgctttgtgg aatcagaggg
     4021 ttatttgtca tttttattgt acctgacgaa gttgcgtgtt gaaggcacta taacacgatg
     4081 ctagtatttg gcaatcaaga cgtttagatg tctaaataaa acaataagga caacacaaca
     4141 tgcctcacaa tcctatccgc gtggtcgtcg gcccggctaa ctacttttca catcccggaa
     4201 gtttcaatca cctgcacgat tttttcactg atgaacaact ttctcgcgcg gtgtggatct
     4261 acggcgaacg cgccattgct gcggcacaaa ccaaactgcc gcctgcgttt gaactgccag
     4321 gggtaaagca tattttgttt cgtggtcatt gcagcgaaag cgacgtgcaa caactggcgg
     4381 cggagtccgg tgacgatcgc agcgtggtga ttggcgtcgg cggcggcgca ctactcgaca
     4441 ccgcgaaagc cctcgcccgc cgtctcggtc tgccatttgt tgccgttccg acgatcgccg
     4501 ccacctgcgc cgcctggaca ccgctctccg tctggtataa cgatgccgga caagcgctgc
     4561 attatgagat tttcgacgac gccaatttta tggtgctgct ggagccagag attatcctca
     4621 atgcgccaca agaatatcta ctggcgggga tcggtgacac gctggcgaaa tggtatgaag
     4681 cggtggtgct ggctccgcaa ccagaaacgt tgccgctaac cgtgcggctg ggaatcaata
     4741 atgcgcaggc cattcgcgac gtcttgttaa acagtaccga acaggcgctt gccgatcagc
     4801 aaaatcagca attaacgcaa tcattttgcg atgtggtgga tgccattatt gctggtggtg
     4861 ggatggtcgg tggtctgggc gatcgtttta cgcgtgtggc ggcggcccat gccgtgcata
     4921 acggtctgac cgttttgccg caaaccgaga agtttctcca cggcaccaaa gtcgcttacg
     4981 gaattctggt gcaaagcgcc ttgctgggtc aggatgatgt gctggcgcaa ttaacagagg
     5041 cgtatcagcg ttttcatctg ccgactacgc tggcggagct ggaagtggat atcaataatc
     5101 aggtggagat cgacaaaatg attgcccaca ccctacgtcc ggtggaatcc attcattacc
     5161 tgccagtcac gctgacacca gataccttgc gtgcagcgtt cgaaaaagtg gaatcgttta
     5221 aagcctgacg tacaaattta gcagcagcgc gcgccgcctt ttccaccata gcgcgcgtcc
     5281 tgccgctcac ggaaaaactc ctcgtaggtc atcggcgttt gatcgggatg gttaacccgc
     5341 atatgttcga catagttgtc gtaatcaggc ataccaatca tcagtttcgc cgcctgacct
     5401 aaatattttc cggcttttgc cagtgaatca aacatctaaa cctccggtaa tacaaatgcc
     5461 ctcctgaatt aagagggctt aattgctatc aacacgctcg ggctgagagt aaggggaacg
     5521 ttcagcacat cccccctcac tctgacttta gtgcgcgcct ttcgcctgcg ccacgatctc
     5581 ctcgacattt tccggcatcg gctcatatgg cgtttctttc gccgttggct tcggctcttt
     5641 caatgccgcc agtgccgtct taatcgagaa cagcgccaga accaccacga ccaccataaa
     5701 gaagatggtt aacccggcat ccagacggtt gttgaacacc agttgcgcca gttgtgactc
     5761 agtatactgc gatggaatat tgccgctgtc gatcattgcc tggaacttgt tagcaatcgc
     5821 caggaatccg actttcgcat ccgggctaaa cgctttctgc cagcctgcgg tcagggtaca
     5881 aatcagcagc caggccgttg gtaccagcgc cacccaggcg taacgttgac gtttcatttt
     5941 gaacagcacc acagcacaaa gcatcagcgc catccctgcc agcatctggt tggcaatacc
     6001 aaacagcggc cacaaggtgt taataccgcc cagcggatcg acctcgccct gatgcaggaa
     6061 gtagccccac gccagcacgc acagcgccgt tgccagcagg ttagcaggca gtgaatcggt
     6121 ccgtttcagg cccggagaca ccacgcccag cagatcctgc aacataaagc gcgcagcacg
     6181 cgtacccgca tccaccgccg tcagaataaa cagtgcttca aacagaatgg cgaagtgata
     6241 ccagaacgcc acatccatca tgccgcccag cgcaccgtgc agaatgtagg ccatccccac
     6301 cgccagggtc ggcgcaccgc ccgcacggga aatgatcgac tgttcaccca cttcgctggc
     6361 aatctggttt agcgtatccg gggtaatcgc aaagccccag ctactcacca cctgcgcggc
     6421 agaagcaacc acatccgccg tccctgccgg agccagcacc gccatcgggc tgttcatggc
     6481 gaagtacaca cccggatcga tgatacaggc ggaaaccagt gccataatcg ccacaaagga
     6541 ttccattaac atcccgccgt agccgataaa gcacgcctgc ccttcgttcg ccagcatctt
     6601 cggcgtggtg ccggaagaga tcagcgcatg gaagccagac accgcgccac aggcgatggt
     6661 gataaacagg aacgggaaca ggttaccggt ccataccggg ccagtgccat cgacaaattt
     6721 agttagcgca ggcatggtca gcgtcgggcg cataattaaa atgcccaccg ccagaccgac
     6781 gatagtcccg attttcagga aggttgagag gtagtcacgc ggagccagca gcaaccacac
     6841 cggcagcacc gccgccacaa aaccgtagcc caccagcatc caggtcagct gcacgccggt
     6901 aaagtcaaag tacggtgccc aggtcgggct ttctgccacc cagccgccag agataatggc
     6961 gaaaatcagg aacaccagac caattaccga cacttcgcca atacgccccg gacgcaaata
     7021 gcgcaggtag atccccataa acagcgccag cggaatggtg aacgcaacgg tatacgttcc
     7081 ccacgggcta tgagtcaggg ctttcaccac aatcatcgcc agcacggcaa ggataatgac
     7141 catgatcata aagcaggcca ccagcgctat caccccggcg gttggtccca tctcttcttt
     7201 gaccagctca cccagcgagc gaccgtcacg gcgcgtagaa acaaacagca ccatgaaatc
     7261 ctgcaccgca ccggcgagaa ccaccccggc gagcagccag atcatccctg gcaggtaacc
     7321 catttgcgcc gccagtaccg gcccaaccaa cggacctgct ccggcaatgg ccgcaaaatg
     7381 gtgaccgaac agcacttttt tgtccgtcgg cacatagtcc agcccgtcgt tatggcgcac
     7441 cgctggcgtc atacgcgtcg ggtcaaccgc cagcacattt ttggcgatat acaggccata
     7501 aaaacggtag gcgattagat agatacagac cgacgccacc acaatccaca gcgcgttgat
     7561 ctgttcccca cgatttaaag caatgtatcc cagagcaaat gctcccatta cagcgagcac
     7621 tgtccagacg aggtatttcc ctgatttgtt catagttgtt atccgtgtgc gtgtccatag
     7681 agatgttaca ttttgtttct ataacatctt tgtacaattt aacaacacag caaccatgta
     7741 aatcggaatt gaaaccaata tttaacttaa caatgttaac tcgatcactc cgttaaccga
     7801 gttttcctgc aatctcattt aattctgtcg ggctcatccc aaaactgccg tacccagccg
     7861 acaggtgcag cagcgccgcc cctgttcatc gaaaacgacg atttcccagc tctgattttg
     7921 tcgtccaaga tgcagcggct ggcagacacc gcgcaccttt ccctcagaca ccgggcgatg
     7981 atgtgtggcg ttaagttccg tgcccaccac acactgtccg tcgcgggtca tcataaatcc
     8041 ggccatcgat cccagcgttt ccgccagcgc cgccgacgcg ccgccatgca gcaggccaaa
     8101 cggctgatga gtacgggtat caaccggcat ttcggcttcg agtacatcat cgcccagacg
     8161 ggtatacaca attcccagat gcgccaccat tgtgttatcg ctggtggcgt tcagctcgtc
     8221 gagcgttaaa tgacgtttcc agatcatgct tatgccccca gcgttgagcc gccatcgacc
     8281 acaatatcct gtagggtaat atggctggcg aggtcagagg cgaggaacaa aatcgtgttg
     8341 gcgatctctt gtggacaggc gattttccct agcggaatgc cgagtttaaa ctgctcgcca
     8401 aagccgcgaa tacgctgctc ttcggcgtca tcgctcaccc acagcgtgcg ttgcatatcg
     8461 gtgtcggtgg agcccggcga aaccacatta cagcgcacgc cgctacccgc cagttccagc
     8521 ccgacgctca acgccaggct tttcagcgcc gctttcgacg cgccataagc actcatacca
     8581 atacgcggcg tgtgtgcggc gtcggacgca acagtaacaa tcgccccgcc ccgctgacgg
     8641 cgaaactggt tcatggtctg ctgaaacagg ttaaacgcac cgccgacgtt gaccgcaaaa
     8701 gtctgctgcc agtcctcttt actgagctga tcggtcgcgc ccatgcgcaa aattcccgcc
     8761 gcattgacca gcacgtccag tcgctccgtt tcagctaaca gtcgctgaca tacctgcgcc
     8821 acctgcgcag catcggcaac atccattact tcggtcgcaa aaggatattg ctcctgacta
     8881 aacgcttgat caaaacctgt aactttcgcc cccgcctcaa caaatgccag cgccgtggcg
     8941 tagcctatac ctttccccgc gccagttacc cagacatttt taccgctgaa atccattatt
     9001 tcacctcgcg ggagagtagc ttccaccagg cgtcgatggt cgggtttttc gccagcatga
     9061 caaagtcgat atcgccatgc actttgcgcc agcgcgccgc cagcgccatc atgcgcaccg
     9121 aatccagacc gtagtcgatc aggttgtcgt catcgaaggg ttcatcggac tcgtccagca
     9181 acggcaggat cacctcacgc agcgccgctt tgctggcggg gataggtgct ggcagcaatt
     9241 cttcggtcat caccacccga ccagaacgtc cggccacata tttcagcgac atcaaatgct
     9301 cgtcacggct gaaatcggcc agcgcatccg ccaccataaa cggtttaata tcgcgcataa
     9361 atgcgtccgt tgcggtggtc atacagccaa tgtgggcata taccccggta ataatcagct
     9421 ggttacgtcc actctctttg agcatttgct ccagcggaga acgatgaaat gcgctgtagc
     9481 gccatttcac cagcaccgtg tcgtcggcat ctggcgtcag gcgatccacc accttttgct
     9541 gttccggcga acgggtcagg ccaggccccc acatatcatt caacagcgcc cgatcttcat
     9601 cgctctgctc tttcggctgg gcggtgtaat aaaccgggat attgtgctgt ttgcagtagt
     9661 cgcgtagtgc cgcaatattc gcgatcacct gctccatcat cgggcagttc tcaccccaga
     9721 agctgacaaa atagtcctgc atatcatgga ttaacaacgc ggcacgttgc ggttcaaagg
     9781 cccagtcgac tttattctgc ggaatatcgt gagactccgg cagtgcgtaa gcctgtaatt
     9841 ttggaatagc catcgtgttc tctccttcag gctgatgtgc gtgacgccag ccactgacgt
     9901 aattgttttt tatcgacttt cccgaccgcc gtcagcggaa gtgaatctac acactccacg
     9961 cgatccggta atttaaattc ggcaataccc tgttcacgca ggaaacgacg cacctgcacc
    10021 gcgcgcagcg gctcttttac caccagataa gcacagcttt tttcgcccat cagctcatct
    10081 tccatgctca ccagtgcggc gtagatcacc gccggatggc gcagcagcag gttttcgatc
    10141 tcttcggcag cgatcttctc gccgccacgg ttgatctgat ctttctcgcg cccctgcaca
    10201 gtgatgtaac cctctggatc aatagagatc agatcgccgg aacagtaaaa accgttagca
    10261 tcaaaggcgc tggcattgtg ctgcgggctt ttgtaatagc cacggaaggt gtacggcccg
    10321 cgcgtcatca ggcgtccaac ttccccttgc ggcagtggat ttccttcggc atcggcaacc
    10381 catacttcat cgtccgggca cattgggtaa ccctgggtat ggataatttt ctccgcacta
    10441 tcatcaagac gggtgtagtt caccagccct tccgccatgc caaacacctg ctgcaactga
    10501 cagccaatct cagcgggaat acgcgccgca agggtggcag aaagacgggc gccgccgacc
    10561 tgtaacagtt tcagcgaggc aagctgcgcc cggctttcgc cttcagccag tgcctgcaac
    10621 cacaggctga ctgctggcgg caccagcgcg gtgacgttca cctgatgttt ttcaatcaat
    10681 gggaagcaaa gcgtggcgct ggggtcggca gccagaacga cagtgccgcc agcgagaaat
    10741 acccccaacg atcccggtga actcatggcg tagttatgag ccgccgggat cgcgcacagg
    10801 tagcgcgtct gttgtgtgaa ctgacaaatc tcgacgctac gacgcacgct gtagtagtag
    10861 tcgttatgag tgcgcgggat cagtttcgga gtgccggtgg tgccgccgga aagctggaaa
    10921 taggccactt catcagcagg agatggcgtg gcagtaaaat cctcagccgg atggttaatc
    10981 gcatcctgca agttatgctc accgctgtcg ttgagcagct gcaccacgcg aatggaagaa
    11041 tgctctgcaa caaatgtgtt gaggaaatca tccccgctaa atagcgcatg ttggcgatcg
    11101 gcaatcagta acgccggttc aatctggctg gcataggcgt tcagttcact acgctgatgg
    11161 ctgaacaacg ccagcaccgg cgcaacgccc agtttcagca gcgcgaaaaa ggtaatgtaa
    11221 agttcagcga cgttacccag ttgtaccagc gcggtttcac caggtttaat gccctgacgg
    11281 cgcaaactac aggcgaggtt atccgccgcc tgattcagct cccggtaact caactgccac
    11341 tcgccgtcga tcaccgcgat gctgtcactc gtagcgtggc gagtcagaat gtcggtcagt
    11401 ggcaaatcct gccagtagcc tttttcccga tagcgacggg caaactcttc cggccagcgg
    11461 gtgaatggaa tgctcatcct cgctccttaa tgcaatccaa aaacgttcaa catggtagaa
    11521 agtttgacgc ccgtttcgcg ccactcaccc aacggtgagg acgcaggcac aatccccgct
    11581 ccggcaaaca gacgcacctg attttcccgc agcttcgcgc agcggatggt caccacccat
    11641 tcaccgttac cttcgctgtc acaccaaccc acaatgccgc caaacagttc gcggtcgaac
    11701 ggctccagtt cagcaataac ctgagtcgcg gcctgatgcg ggaaaccgct cagcgcgggg
    11761 gtcggatgca gcagacaagc caaagtcagt gcgttttctt gcgaattcgc tttaccttca
    11821 aagggagttg cgagatgcca cagcgtcggc gtggtaatca attgtggaga ggagggaacg
    11881 tgtaactcac tactgcgttt gcgcagtacc tctttcatcg cctgagtcac cagttcatgt
    11941 tcatggcgat ctttttctga cgccagcaga cgattacccg cttcgcgatc gagcacttca
    12001 tccggctgac gacgcgcgga accggctaac ggaatggagc taaaacgctc gccgtcttta
    12061 cgtagcagca gttccgggct ggcccccagc agaacgccac catcagccaa cggaacatgg
    12121 aagttgtaac taaccgggtt ttgcgcaatc aaccgttcca gcaatacgcc actatcaatg
    12181 gcggcgtcag tggtgatatc aatcaaccgt gacaacacca ctttgtcgac ctgcggcgtg
    12241 gcggtaagtg cggcagcacg ggcaaccatc tgttcaaacg tggtttgttc cggaattgcc
    12301 tgacgttcca ccacgttcag cgactggctg cgagtgaaac ggcgtgcgga agcttgtttt
    12361 tcctgacggg agaacgactg ccaggattcg ggtatataca gcgacgaagg ctgacgtgga
    12421 tcgaagggaa tcgccccgac catcaccgga tttttgatgc cctgcgcttt ggcatcggca
    12481 aacagcgcgg cgagtttttg ctggaaggga ctgtcgggcg aatccccgtt cacagccggt
    12541 tcatcgaagc gggcgaaaca tcctgacgtc gtaaaactgc ggtacggcga cataaagaaa
    12601 aagcgattgg gcgcaagtgt tgccatggtc tgctgtactt cctcagccag tgacgtatcc
    12661 atatcatcct ccacaaaatg ataaaggctt taataatgat tatcatttat attttcggtc
    12721 gctaacctaa aagcaaacgc gcactatgtc aactcttgag gtaacgcaat ctgcaactcg
    12781 gggttgcatc tggttgtggt tacaatgaaa atgagaagca ttaatttcat taataacagg
    12841 aagctgactt gtgagactcg ccccgctcta ccgcaacgcc cttctattaa caggactttt
    12901 gctttcagga atagccgcag ttcaggccgc cgactggccg cgtcagatta ctgacagccg
    12961 tggcactcat accctggaaa gccagccgca gcgtatagtt tccaccagcg tcaccctgac
    13021 cggctcactg ctggcgattg atgctccggt gatcgccagc ggcgcgacca cgccaaataa
    13081 ccgcgtcgcg gatgaccagg gctttttacg ccagtggagc aaggtggcga aagaacgcaa
    13141 actgcaacgg ctctatatcg gcgaaccgag tgcagaagcc gttgccgcgc aaatgccgga
    13201 tctgatttta attagcgcaa ccggcggcga ttcggcgctg gcgctgtacg atcagctttc
    13261 tactatcgcc ccaacattaa tcatcaatta cgacgacaaa agctggcagt cgctgttaac
    13321 gcagcttggc gaaattaccg ggcatgagaa acaagcggca gagcggattg cacagtttga
    13381 taagcaactg gcggcggcga aagagcaaat caaattaccg ccgcagccgg tcactgccat
    13441 cgtctatacc gccgctgcac acagtgccaa tctctggacg ccggaatcag cacaagggca
    13501 gatgctggaa caactcggct ttacgctggc gaagttgccc gcagggttaa acgccagcca
    13561 aagccagggc aaacgccatg acatcattca gcttggtgga gagaatctgg ctgcagggtt
    13621 aaatggcgag tcactattcc tgttcgccgg tgatcagaaa gacgccgatg ctatttatgc
    13681 taatccgctg ctcgcgcacc tgcctgcagt acaaaacaag caggtttatg cgctgggaac
    13741 cgagacgttc cgcctggatt actacagcgc catgcaagtg ctggatagac ttaaggcgct
    13801 gttttaagca ttaactgtcg gacgctgtca cctgcggcgg cgtctggcga aaacgccgca
    13861 actccaccag caccagcaac aacaacacgc cgataatcaa caaaccaaaa ccgctcgcac
    13921 ttgcggaagc aaccggtgtc atcatcgctc ccaaaccgcc cagcagcgcc gcgcctatgg
    13981 catcgcccgt cacgttttgc gccgtccaca aaccgttaat ccgccctaac atcgcttccg
    14041 gggtttgcgt ttgcagcatt gtatattgca gcaacgaact aaccgcactc agccagccga
    14101 acagtgccag acaaaccacg cctaaaatcc acatcggcat caggccaaac agaccaatgg
    14161 cgaggaacgc ccccaatgtg gagagcaaca tcaacagacc tggccgtacg ctatgcgcca
    14221 gttttccact ggttaacgcg ccaatagccg caccgagcgg gattgccgca tagagaaagc
    14281 caatttgcgc cgccgacatc tgccagttgt cagccagcgc cggatacagg acccgcaccg
    14341 cgctcgccat cgtcaataaa ccccccagca acgcaatccc tcccaccagc gggctggcga
    14401 gtagaaaacg aaatcctgcc agtaatgatt tcaacggatg ctcacgcggc tgcggcggtg
    14461 gtggcaacgc cggaaggctt aacaacggta gcaaggtaat aaatgtgccc gccgccgcca
    14521 gcccgtagtt ccaggctacg cctccggtcg ccagcaataa accgccaatc atgggcgaaa
    14581 tcaccgatcc cagacgcaca gtcaacatgg tgatcgcccc ggcctgcatt aggttttcac
    14641 gccctaccag tgcaggtgtc gccgccagta gcgccgtaac gccaagtgat gcgaaaaaac
    14701 catcccataa accaagtaaa tagattgcca gcaaagacgg ctccggcagc agcgcattca
    14761 ggcacagccc aatgaagcca atgccacagg taccgcgcgc cagcaaaatc acttttttac
    14821 gctcatagcg atccgccagc acaccgccga ccatcaggcc aacaaacatc gcgccgccgg
    14881 tcagcgtcac cgaaagcccc acctgccagg tagaatgcgt cattatctgg atctgcaccg
    14941 gcaccgcgac gccgagcaaa cccagagaaa caattgagat aaaacgagcg aggaatactg
    15001 cgcgaaacgc cgggtgcgtt ttcaacaggc tgaggttaag cagccaggat tgtttattca
    15061 ttacaatgcc ttgccatcaa atattttaaa tacctattct taggctgcac atgctaacat
    15121 atccaaataa gatcgataac gataattaat ttcattatca tggaagttcg tatgtctggt
    15181 tctgttgccg tgacacgcgc tattgccgtg cccggattgc tgttattact gattatcgcg
    15241 acggcattaa gcctgctcat tggagcaaaa tcactccccg cttccgtagt gctggaagcc
    15301 ctctccggca cctgccagag cgccgactgc accatcgtgc tcgacgcacg actgccgcgc
    15361 acccttgccg gtttactggc aggcggtgcg cttggccttg ccggggcgtt aatgcaaacc
    15421 ctcacccgaa acccacttgc cgaccccggc ttacttggcg tgaatgccgg agccagcttt
    15481 gccattgtgc tgggtgcggc gctgtttggt tactcttccg cgcaggaaca actgatgatg
    15541 gccttcgccg gcgcgctggt ggcgtcattg attgttgcct ttaccggcag ccagggcggc
    15601 gggcagttaa gtccggtgcg tttaaccctg gcgggcgtgg cgctggcggc ggtgctggaa
    15661 gggctgacca gcggcatcgc cctgcttaat cctgacgtct acgatcaact gcgtttctgg
    15721 caagccggtt cgctggatat tcgcaatcta cataccttaa aagtggtgct gatcccggtg
    15781 cttaccgccg gagcaaccgc gctattactg agccgcgcgc tgaacagttt aagccttggc
    15841 agtgacaccg cgacggcgct gggcagtcgc gtagcgcgca cacagttgat tggtctgctg
    15901 gcaattaccg tgctttgtgg tagtgcgacg gcggtagttg gcccgattgc ctttattggc
    15961 ctgatgatgc cgcacatggc gcgctggctg gtaggtgccg atcatcgctg gtcgctgccc
    16021 gtcacgctac tcgctacccc tgccctgctg ctgtttgccg atatcattgg gcgggtgatt
    16081 gttcccggcg aactgcgcgt ttctgtagtc agtgccttta ttggtgcacc agtgctgatc
    16141 ttcctcgtac gacgtaaaac gcgaggtggc gcatgattta cgtctctcgc cgattaatta
    16201 tcacctgtct actgttgcta atagcttgtg taatggcagg cgtgtgggga ttacgcagcg
    16261 gtgccgtcac gctggaaacc tcgcaggtat tcgccgcgct gatgggcgat gcgccgcgca
    16321 gtatgacgat ggtggtcacc gaatggcgtt taccacgcgt gctgatggcg ctgttgattg
    16381 gcgcggcgct gggcgtcagc ggcgcgattt ttcagtcgct gatgcgtaac ccgctcggca
    16441 gccctgacgt aatgggcttt aacaccgggg cgtggagcgg cgtactggtg gcgatggtgc
    16501 tgtttggtca ggacctgacg gctatcgccc tggcagcaat ggcggggggt attgtcacct
    16561 cgctgctggt ctggctgctc gcctggcgta acggtatcga cacctttcgg ttgattatta
    16621 tcggtatcgg cgttcgcgcc atgctggtgg cctttaatac ctggctgttg ctgaaagcgt
    16681 ctttagaaac ggcgctaaca gcgggtttgt ggaatgccgg atcgctcaac ggcctgacgt
    16741 gggcaaaaac ctcgccttcc gcacccatca ttatattaat gttaattgcc gctgccttgc
    16801 tggtgcggcg aatgcgcttg ctggaaatgg gcgatgatac cgcgtgtgcg ctgggcgtca
    16861 gcgtcgaacg ttcgcggctg ttaatgatgc tggtcgccgt agtgcttacc gcagctgcca
    16921 ctgccctggc ggggccaatt tcctttattg ctttagtcgc gccacacatt gcccgccgca
    16981 tcagtggcac cgctcgctgg gggctgaccc aggcggcgct gtgcggggca cttttactgc
    17041 tggcggccga tctctgcgcc caacaactgt ttatgccgta tcaacttccg gttggcgtcg
    17101 ttaccgtcag cctcggcggt atttacctta tcgtcttgtt aattcaggag tctcgcaaaa
    17161 aatgaccgaa tcagtagccc gtttgcgtgg cgaacagtta accctcggat atggcaaata
    17221 taccgttgcg gaaaatctga ctgtagaaat acctgatggt cacttcacgg caattatcgg
    17281 gccaaatggc tgcggtaaat ccacgttact gcgtacctta agccgcctga tgacgcctgc
    17341 tcacgggcat gtctggctgg atggcgagca cattcaacat tacgccagta aagaggttgc
    17401 acgccggatt ggtctgttgg cgcaaaatgc taccacaccg ggcgatatca ccgtgcagga
    17461 gctggtggcg cgtgggcgtt acccgcatca accgctgttt acccgctggc gcaaagaaga
    17521 tgaagacgcg gtaacaaaag cgatgcaggc cacgggaata actcatctgg cggatcaaag
    17581 cgtggacacc ctttctggcg gacaacgcca gcgagcgtgg atcgcgatgg tgctggccca
    17641 ggaaacggca attatgctgc tcgacgaacc cacgacctgg ctggatatca gtcatcagat
    17701 tgatctactg gaactgttaa gcgaactgaa ccgcgagaaa ggctataccc tggcggcggt
    17761 gctgcacgat cttaatcagg cctgtcgtta cgccagccat ttgattgcat tgcgggaagg
    17821 gaaaattgtt gctcagggag cgccgaaaga gattgtcact gctgaactga tcgagcgcat
    17881 ttatggtctg cgctgcatga taattgacga tccggttgcc ggaacgccgc tcgtggtgcc
    17941 gctcggacga acggcacctt caaccgcaaa gatttaaact aagtggtctg ccatcatggc
    18001 atcctgtttt ctggatgcca tcgcatgacg caacagcaca ccaccacaag ccaccatccc
    18061 gccgatcaac gcggaaagga tcacaataat agccttaccc ggaccgtctt ttttcaccgg
    18121 caacgatggg cttaattgat atttaaacgg aatgaattta acgtcatcaa catttgcttt
    18181 tgttaattgc tcaacaagat attgacgatt acgtaattca ccgttcaatt ccgcaacgtc
    18241 agtaaccgct ttttcaattt ccagtttacg accaatacca tcagcgccga gagaaataga
    18301 gaaatcggga tcatctttaa cggcctgacc attactgtat acgggctttt taattcctgc
    18361 cgcgttggca atgtcgagtg aataattgag gcgctgaatg tttgcatcaa gttgattttt
    18421 cgttttaatg cgatcctgag ccagtttttc tttttcaaac tgggttttga tctccagttt
    18481 attacggacg ttttctatcg actctttcac caccaacgca gagatataat cgatataccc
    18541 gctcaaaacg gtctgcgcct cttcactggt tggcgcggta aaacttagcg tccaggaggt
    18601 atacagtgac ggttcatctt ttttcttact ggcattgtca tcaaccgctt tcattttttc
    18661 gctcaatgcg acgatagcgc gatgcaaatc cagttcgtcg attttcgctt cttttaattg
    18721 atccatcaca taaggtgatg agcgcaggta ctcttctagt aagctaacag actgaaattt
    18781 cttgataaac aggttaaata cttctgtacg atcaattttg atatccagat ccagcacacg
    18841 aagcttagtg aatgttttct ccagctcttg ccactgaaca ggttctggag gcgtgacaac
    18901 cgccgcgctg gtccattttt gcggcaggat gaaagagatc agcaatcctg tgcaggcaaa
    18961 cgcaaaaatg accgccatga ccgttttttt tgcccgccat aaaacttcga ttagattaag
    19021 taaatcaatt tcattattac tgggcgacgc cagaggatac tcgggaaaat gagcttcact
    19081 tccctgttta atattcagtg atgacatggg gtataacctg aaaatgagtc caataagagt
    19141 aaatctccac ggcccccact ctaccagttg gtgagattta tccgaagctg aagtttgtaa
    19201 attactccca ctgaatgcaa cattcactct atttcagaga aatacccacc aaatttataa
    19261 attaaacaaa
//
LOCUS       DAHHTG010000082        19248 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.082, whole genome shotgun sequence.
ACCESSION   DAHHTG010000082 DAHHTG010000000
VERSION     DAHHTG010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19248)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19248)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19248
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.082"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            complement(92..3538)
                     /gene="mfd"
                     /locus_tag="NBI74_002987"
     CDS             complement(92..3538)
                     /gene="mfd"
                     /locus_tag="NBI74_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HCD1933280.1"
                     /translation="MPEQYRYTLPVKAGEQRLLGELTGAACATLVAEIAERHAGPVVL
                     IAPDMQNALRLHDEISQFTDQMVMNLADWETLPYDSFSPHQDIISSRLSTLYQLPSMQ
                     RGVLIVPVNTLMQRVCPHSFLHGHALVMKKGQRLSRDALRTQLDSAGYRHVDQVMEHG
                     EYATRGALLDLFPMGSELPYRLDFFDDEIDSLRVFDVDSQRTLEEVEAINLLPAHEFP
                     TDKAAIELFRSQWRDTFEVKRDPEHIYQQVSKGTLPAGIEYWQPLFFSEPLPPLFSYF
                     PANTLLVNTGDLENSAERFQADTLARFENRGIDPMRPLLPPQSLWLRVDELFSELKNW
                     PRVQLKTEHLPTKAANANLGFQKLPDLAIQAQQKAPLDALRKFLESFDGPVVFSVESE
                     GRREALGELLARIKIAPQRIMRLDEASDRGRYLMIGAAEHGFVDTVRNLALICESDLL
                     GERVARRRQDSRRAINPDTLIRNLAELHIGQPVVHLEHGVGRYAGMTTLEAGGITGEY
                     LMLTYANDAKLYVPVSSLHLISRYAGGAEENAPLHKLGGDAWSRARQKAAEKVRDVAA
                     ELLDIYAQRAAKEGFAFKHDREQYQLFCDSFPFETTPDQAQAINAVLSDMCQPLAMDR
                     LVCGDVGFGKTEVAMRAAFMAVDNHKQVAVLVPTTLLAQQHYDNFRDRFANWPVRIEM
                     ISRFRSAKEQTQILAEVAEGKIDILIGTHKLLQSDVKFKDLGLLIVDEEHRFGVRHKE
                     RIKAMRANVDILTLTATPIPRTLNMAMSGMRDLSIIATPPARRLAVKTFVREYDSLVV
                     REAILREILRGGQVYYLYNDVENIQKAAERLAELVPEARIAIGHGQMRERELERVMND
                     FHHQRFNVLVCTTIIETGIDIPTANTIIIERADHFGLAQLHQLRGRVGRSHHQAYAWL
                     LTPHPKAMTTDAQKRLEAIASLEDLGAGFALATHDLEIRGAGELLGEEQSGSMETIGF
                     SLYMELLENAVDALKAGREPSLEDLTSQQTEVELRMPSLLPDDFIPDVNTRLSFYKRI
                     ASAKTENELEEIKVELIDRFGLLPDPARTLLDIARLRQQAQKLGIRKLEGNEKGGVIE
                     FAEKNHVNPAWLIGLLQKQPQHYRLDGPTRLKFIQDLSERKTRIEWVRQFMRELEENA
                     IA"
     gene            complement(3665..4738)
                     /locus_tag="NBI74_002988"
     CDS             complement(3665..4738)
                     /locus_tag="NBI74_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HCD1933281.1"
                     /translation="MKQKELWINQIKGLCICLVVIYHSVITFYPHLTTFQHPLSEVLS
                     KCWIYFNLYLAPFRMPVFFFISGYLIRRYIDSVPWGNCLDKRIWNIFWVLALWGVVQW
                     LALSALNQWLAPERDLSNASNAAYADSTGEFLHGMITASTSLWYLYALIVYFVICKIF
                     NPLALPLFVLFVLMSVAVNFVPTPWWGMNSVIRNLPYYSLGAWFGATLMTCVKAVPLR
                     RHLLMASLLTVLAVGAWLFNISLLLSLVSIVVIMKLFYQYEQRFGMRSSSLLNVIGSN
                     TIAIYTTHRILVEIFSLSLIPQMNAVRWSPQVELTLLLIYPFVSLLICTVAGLLVRKL
                     SQRAFSDLLFSPPSLPATVSYSR"
     gene            4999..6198
                     /gene="lolC"
                     /locus_tag="NBI74_002989"
     CDS             4999..6198
                     /gene="lolC"
                     /locus_tag="NBI74_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004976588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolC"
                     /protein_id="HCD1933282.1"
                     /translation="MYQPVALFIGLRYMRGRAADRFGRFVSWLSTIGITLGVMALVTV
                     LSVMNGFERELQNNILGLMPQAILSSEHGSLNPQQLPETAVKLDGVNRVAPITTGDVV
                     LQSARSVAVGVMLGIDPAQKDPLTPYLVNVKQTDLEPGKYNVILGEQLASQLGVNRGD
                     QIRVMVPSASQFTPMGRIPSQRLFNVIGTFAANSEVDGYEMLVNIEDASRLMRYPAGN
                     ITGWRLWLDEPLKVDSLSQQKLPEGSKWQDWRDRKGELFQAVRMEKNMMGLLLSLIVA
                     VAAFNIITSLGLMVMEKQGEVAILQTQGLTPRQIMMVFMVQGASAGIIGAILGAALGA
                     LLASQLNNLMPIIGVLLDGAALPVAIEPLQVIVIALVAMAIALLSTLYPSWRAAATQP
                     AEALRYE"
     gene            6191..6892
                     /gene="lolD"
                     /locus_tag="NBI74_002990"
     CDS             6191..6892
                     /gene="lolD"
                     /locus_tag="NBI74_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309522.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter
                     ATP-binding protein LolD"
                     /protein_id="HCD1933283.1"
                     /translation="MNKILLQCDNLCKRYQEGSVQTDVLHNVSFSVGEGEMMAIVGSS
                     GSGKSTLLHLLGGLDTPTSGDVIFNGQPMSKLSSAAKAELRNQKLGFIYQFHHLLPDF
                     TALENVAMPLLIGKKKPAEINSRALEMLKAVGLEHRANHRPSELSGGERQRVAIARAL
                     VNNPRLVLADEPTGNLDARNADSIFQLLGELNRLQGTAFLVVTHDLQLAKRMSRQLEM
                     RDGRLTAELSLMGAE"
     gene            6892..8136
                     /gene="lolE"
                     /locus_tag="NBI74_002991"
     CDS             6892..8136
                     /gene="lolE"
                     /locus_tag="NBI74_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolE"
                     /protein_id="HCD1933284.1"
                     /translation="MAMPLSLLIGLRFSRGRRRGGMVSLISIISTIGIALGVAVLIVG
                     LSAMNGFERELNNRILAVVPHGEIEAVDQPWTNWQEALDNVQKVPGIAAAAPYINFTG
                     LVESGANLRAIQVKGVNPQQEQRLSALPSFVQDDAWSNFKAGEQQIIIGKGVADALKV
                     KQGDWVSIMIPNSNPEHKLMQPKRVRLHVAGILQLSGQLDHSFAMIPLADAQQYLDMG
                     SSVSGIALKMTDVFNANKLVRDAGEVTNSYVYIKSWIGTYGYMYRDIQMIRAIMYLAM
                     VLVIGVACFNIVSTLVMAVKDKSGDIAVLRTLGAKDGLIRAIFVWYGLLAGLFGSLCG
                     VIIGVVVSLQLTPIIEWIEKLIGHQFLSSDIYFIDFLPSELHWLDVFYVLVTALLLSL
                     LASWYPARRASNIDPARVLSGQ"
     gene            8165..9076
                     /gene="nagK"
                     /locus_tag="NBI74_002992"
     CDS             8165..9076
                     /gene="nagK"
                     /locus_tag="NBI74_002992"
                     /EC_number="2.7.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine kinase"
                     /protein_id="HCD1933285.1"
                     /translation="MYYGFDIGGTKIALGVFDSGRQLQWEKRVPTPRDSYDAFLDAVC
                     ELVAEADQRFGCKGSVGIGIPGMPETEDGTLYAANVPAASGKPLRADLSARLDRDVRL
                     DNDANCFALSEAWDDEFTQYPLVMGLILGTGVGGGLIFNGKPITGKSYITGEFGHMRL
                     PVDALTMMGLDFPLRRCGCGQHGCIENYLSGRGFAWLYQHYYHQPLQAPEIIALYDQG
                     DEQARAHVERYLDLLAVCLGNILTIVDPDLVVIGGGLSNFPAITTQLAERLPRHLLPV
                     ARVPRIERARHGDAGGMRGAAFLHLTD"
     gene            9092..9913
                     /gene="cobB"
                     /locus_tag="NBI74_002993"
     CDS             9092..9913
                     /gene="cobB"
                     /locus_tag="NBI74_002993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HCD1933286.1"
                     /translation="MLSRRGHRLSRFRKNKRRLRERLRQRIFFRDKVVPEAMEKPRVL
                     VLTGAGISAESGIRTFRAADGLWEEHRVEDVATPEGFDRDPELVQTFYNARRRQLQQP
                     EIQPNAAHLALAKLQDALGDRFLLVTQNIDNLHERAGNTNVIHMHGELLKVRCSQSGQ
                     VLDWTGDVTPEDKCHCCQFPAPLRPHVVWFGEMPLGMDEIYMALSMADIFIAIGTSGH
                     VYPAAGFVHEAKLHGAHTVELNLEPSQVGNEFAEKYYGPASQVVPEFVEKLLKGL"
     gene            complement(10051..10839)
                     /locus_tag="NBI74_002994"
     CDS             complement(10051..10839)
                     /locus_tag="NBI74_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HCD1933287.1"
                     /translation="MKKIIILLSLLILLPLTATSKPLIPIMKTLFTDVTGTVPDAEEI
                     AHKAELFRQQTGVAPFIVVLPDINNEASLRQNGKAMLAHAASSMSNVKGSVLLLFTTR
                     EPRLIMITNGQVESGLDDKHLGLLVENHTLAYLHADLWYQGINNALAVLQAQILKQPT
                     PPLTYYPHPGQQHENDPPGSTTTLGLFAWAVAFIVFAAFFNYTTRLYYALKFAVAMAV
                     ANMGYQALCLYIDNSFAITRISPLWAGLIGVCTFIAALLWTSKR"
     gene            complement(10836..11297)
                     /locus_tag="NBI74_002995"
     CDS             complement(10836..11297)
                     /locus_tag="NBI74_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3592 domain-containing protein"
                     /protein_id="HCD1933288.1"
                     /translation="MSQDSRVFFRIFFGIGLVLILISVVIFYNQFTYSKDAIHTEGVI
                     VDTVWHSSHSHRTGKNGSWYPVVAFRPTPDYTLIFNSSIGSDFYEGSEGDKVNVYYSP
                     GHPEKAEINNPWVNFFKWGFIGIMGVIFISVGLLISMPSSKKTRRKRKSRP"
     gene            complement(11355..12401)
                     /gene="potD"
                     /locus_tag="NBI74_002996"
     CDS             complement(11355..12401)
                     /gene="potD"
                     /locus_tag="NBI74_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotD"
                     /protein_id="HCD1933289.1"
                     /translation="MKKWSRHLLAAGALALGMSAAHADDNNTLYFYNWTEYVPPGLLE
                     QFTKETGIKVIYSTYESNETMYAKLKTYKDGAYDLVVPSTYYVDKMRKEGMIQKIDKS
                     KLSNFSNLDPDMLNKPFDPNNDYSIPYIWGATAIGVNGDAVDPKSVTSWADLWKPEYK
                     GSLLLTDDAREVFQMALRKLGYSGNTTDPKEIEAAYNELKKLMPNVAAFNSDNPANPY
                     MEGEVNLGMIWNGSAFVARQAGTPIDVVWPKEGGIFWMDSLAIPANAKNKEGALKLIN
                     FLLRPDVAKQVAETIGYPTPNLAARKLLSPEVANDKTLYPDAETIKNGEWQNDVGAAS
                     SIYEEYYQKLKAGR"
     gene            complement(12398..13192)
                     /gene="potC"
                     /locus_tag="NBI74_002997"
     CDS             complement(12398..13192)
                     /gene="potC"
                     /locus_tag="NBI74_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotC"
                     /protein_id="HCD1933290.1"
                     /translation="MIGRLLRGGFMTAIYAYLYIPIIILIVNSFNSSRFGINWQGFTT
                     KWYSLLMNNDSLLQAAQHSLTMAVFSATFATLIGSLTAVALYRYRFRGKPFVSGMLFV
                     VMMSPDIVMAISLLVLFMLLGIQLGFWSLLFSHITFCLPFVVVTVYSRLKGFDVRMLE
                     AAKDLGASEFTILRKIILPLAMPAVAAGWVLSFTLSMDDVVVSSFVTGPSYEILPLKI
                     YSMVKVGVSPEVNALATILLVLSLVMVIASQLIARDKTKGNTGDVK"
     gene            complement(13359..14477)
                     /locus_tag="NBI74_002998"
     CDS             complement(13359..14477)
                     /locus_tag="NBI74_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase Arm DNA-binding
                     domain-containing protein"
                     /protein_id="HCD1933291.1"
                     /translation="MSPRPRKNSTDVAGLYEKFDRRTGRVYYQYKNPVTGKFHGLGTD
                     KGKAEKIASTANQRIAAAEAEYFMRKIDESPSATKRRGIRLKAWVDRYLKIQDTRLKN
                     GDIAATTHKEKTRMAAYLVSRLGNHPLKELEVRDFALILDEWLDKDMVSTARVNRGLW
                     VDIYKEAQHAGEVPPGWNPPEATRKPIPKVTRARLTMEDWQKIYNATPEKHFIRNAML
                     LAIVTGQRRDDICHMRFSDVWNEHLHITQGKTGMRLALPLTLRCDAIGITLKEVIDGC
                     RDRILSPYLIHSRHQKQPKPMSKDNLSDYFAKARDLAGIIPPAGKTPPTFHEQRSLSE
                     RLYRAQGIDTKTLLGHKVQATTDRYNDTRGQEWVKLVI"
     gene            complement(14446..14715)
                     /locus_tag="NBI74_002999"
     CDS             complement(14446..14715)
                     /locus_tag="NBI74_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HCD1933292.1"
                     /translation="MSEQYLITLDEWKPKRFSLPITNTTLVKYGKLGYIVPRPQKIRG
                     RWLIDRRAVFVGPGETGIAPEIHTGDDDALKEILTHVTEATKKQH"
     gene            complement(14777..17233)
                     /locus_tag="NBI74_003000"
     CDS             complement(14777..17233)
                     /locus_tag="NBI74_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exoribonuclease"
                     /protein_id="HCD1933293.1"
                     /translation="MSTDKEEIALYYEAKNDKIRKRLGIKGGFYWRTAKKLSVAISRG
                     VVAMDDAGFDKEDFKKPVRVHLPVVNDLPPEGVFDTEFCNRYEKGGEDGITMVLIAPS
                     PSVQDKPASTDNTNVNGENMAEIEENMLLPVSGQILPVRWLAQHGSEKPITHVSRDEL
                     RTLHNAQDEKLPAVTALAISNKAAQLEPLEIRDLHKLVRDTDKVFPNPGNSDLGLMTA
                     FFEAYLGADYTDRGLLTKEWMKGNRVSRITRTASGANAGGGNKTDRNPNLVHTFDTLD
                     VEIAAATLPMDFNIYEIPGSVYRRAKEVVRKKESPFKEWSAALRAIPGILDYSRAAIF
                     ALIRSAHPEFYHYPGRLQGYINAYLTETDHENPTEETLAAARHTPEKDILEEVNRELA
                     AERETEEEKNNEEKSQPSDAMADEQATTEAMEPNTTEHRQDTQSLDTQAQIDPVNQVK
                     VTADEVNKIMQAANISQPDADKLLAASRGEFVAGISDPNDPKWVKGMETRDSVNQNQQ
                     ETEQNGQKAEQNSPNALQNEPETKQPEPVVQQEPEKICTACGQTGGGNCPDCGAVMGD
                     ATYQETFNEENQVEVQENDPEEMEGTEHPHKENPGGNQHHDSDSETGEATDHSVKVNG
                     HHKGTSTSGTCDHLMIDLETMGKNPDAPIISIGAIFFDPQTGDMGPEFSKTIDLETAG
                     GVIDRDTIKWWLKQSREAQSAIMTDEIPLDDALLQLREFIDENSGEFFVQVWGNGANF
                     DNTILRRSYERQGIPCPWRYYNDRDVRTIVELGKAIDFDARTAIPFEGERHNALDDAR
                     YQAKYVSVIWQKLIPSQADF"
     gene            complement(17311..17514)
                     /locus_tag="NBI74_003001"
     CDS             complement(17311..17514)
                     /locus_tag="NBI74_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001532593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1482 family protein"
                     /protein_id="HCD1933294.1"
                     /translation="MNTLFVLILTVHLNTGESLDAITGMYNSMKECMAAAAEQKIPGN
                     CYPVDKVIHMDNNEIPAGLKTAP"
     gene            complement(17511..17699)
                     /locus_tag="NBI74_003002"
     CDS             complement(17511..17699)
                     /locus_tag="NBI74_003002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibitor"
                     /protein_id="HCD1933295.1"
                     /translation="METSLPNVNTSDGCFNIGVLLSNRDFTEDAINMRKYEPYLLNDN
                     SILSRIALLKLGIFGEWR"
     gene            complement(17710..18564)
                     /locus_tag="NBI74_003003"
     CDS             complement(17710..18564)
                     /locus_tag="NBI74_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HCD1933296.1"
                     /translation="MLNVAIENQNGWNYSAPAPHKTGAGRGNPEFNQAHNRAQAVFLC
                     VKHSHIQIMVGRAGQLQGWPDSWLTGISTPVRLTTLMVVENLGGELLILNQEAAIMAT
                     IPTLSHPDVTIENGRAVTTSVAVAEFFRKMHKDVLRKIETLECSEDFNERNFTPVTYT
                     DAKGEKRPMYQITKNGFVFLVMGFTGKKAATFKEAYIAEFDRMEEELRQNNAPSPDKM
                     IHGDGRTLVIRLDEHGNIKFTETVPDGAMVCTLDTFRFYLEKQGWTLVNRSAIKNMTV
                     EQLLKIHC"
BASE COUNT         4523 a         4789 c         4886 g         5050 t
ORIGIN      
        1 tatggagggt gacagtgtcg gataatgcag ggaagtgcaa agaatttgta aatattgcag
       61 atgggggcgc agaaacgccc ccgatttacc attaagcgat cgcgttctct tccagttcac
      121 gcataaactg gcgtacccat tcgatacgcg ttttccgctc gctcaaatcc tgaataaact
      181 tcagacgcgt cggaccatca aggcggtaat gctgcggctg tttttgcagc aaaccaatca
      241 accaggccgg attaacgtga ttcttctcgg caaattcgat cacgccgcct ttctcattac
      301 cttccagctt cctgatcccc agtttctgcg cttgctggcg cagacgggca atatccagca
      361 gggtacgcgc cggatccggc agcaggccga agcgatcgat aagctcgact ttgatctctt
      421 ccagttcgtt ttccgttttg gcgctggcaa tacgtttgta gaacgacaga cgcgtgttca
      481 cgtcagggat gaaatcatct ggcaatagcg acggcatccg cagctcgact tctgtttgct
      541 ggctggtgag atcttccagc gacggctcgc gtccggcttt cagcgcatcg acggcgtttt
      601 ccagcaactc catatacagc gagaaaccga tggtttccat tgagccgctt tgttcttcgc
      661 caagcagttc acccgcgccg cggatctcca gatcgtgcgt tgccagcgca aaacctgcac
      721 cgagatcttc cagcgaggca atcgcttcaa gacgtttttg cgcatcggta gtcatcgctt
      781 ttggatgcgg cgtcagcagc catgcatatg cctggtggtg cgaacgcccg acgcgaccgc
      841 gcaactggtg caactgtgcc agaccgaagt gatccgcgcg ttcaatgata atggtgttgg
      901 cggtaggaat gtcgatcccg gtttcgataa tggtggtaca aaccagcacg ttgaaacgtt
      961 gatgatggaa atcattcatc acccgttcca gttcgcgctc acgcatttgc ccgtgaccga
     1021 tagcaatccg cgcttcaggt accagttctg ccagccgttc ggcggctttc tggatgtttt
     1081 ccacatcatt gtagagataa taaacctgtc ctccgcgcaa aatttcacgc aggatcgcct
     1141 cccggaccac cagactgtca tactcacgga caaaggtttt aactgccaga cgacgggcgg
     1201 gcggcgtggc gataatcgac agatcgcgca ttccgctcat tgccatattc agcgtacgtg
     1261 ggatcggcgt tgcggtaagc gtcagaatat ccacgttcgc gcgcatcgct ttaatgcgct
     1321 ctttatgacg caccccgaag cggtgttctt catcgacaat cagcaggcct aaatctttaa
     1381 acttgacgtc actttgcagc agtttgtgcg taccgatcag aatatcaatt ttcccttccg
     1441 ccacttccgc aaggatttgc gtctgctctt tggcgctgcg gaaacgggag atcatttcga
     1501 tacgtaccgg ccagttggcg aaacggtcgc ggaagttgtc gtaatgctgc tgcgcgagaa
     1561 gggtggtagg caccagcacc gccacctgtt tgtggttatc taccgccatg aaagcggcgc
     1621 gcatcgccac ttctgtttta ccaaagccaa catcgccgca caccagacga tccattgcca
     1681 gcggctgaca catgtcacta agcaccgcat taatggcctg cgcctgatcc ggcgtggttt
     1741 caaacgggaa gctgtcgcag aacaactgat actgctcgcg atcgtgttta aacgcgaagc
     1801 cctctttggc ggcgcgttgc gcgtagatat ccagcaattc cgccgccaca tcacgcactt
     1861 tttccgccgc tttctgccgc gcgcgtgacc aggcatcgcc accaagttta tgcagtggtg
     1921 cgttttcttc tgcgccaccc gcgtaacggc taatcagatg cagtgacgac accggaacat
     1981 acagtttggc gtcgttggca taggtgagca tcaaatactc gccagtaatg cctccagctt
     2041 cgagcgtggt cattccggca taacgaccga cgccgtgctc cagatggacc accggctgac
     2101 caatatgcag ctccgcaagg ttacggatca gtgtatcggg gttgatggcg cggcgagaat
     2161 cctgacgacg acgggcaacg cgttcaccga gcagatcgct ttcgcaaatc agcgccagat
     2221 tacgtaccgt atcgacaaaa ccatgctctg cagcgccaat catcagataa cgcccacggt
     2281 cgctggcttc atcaagacgc ataatgcgtt gcggagcaat tttaattcgc gcgagcagtt
     2341 cacccaacgc ttcacggcgg ccttcacttt ctaccgagaa caccaccgga ccgtcgaaag
     2401 actcgaggaa cttacgcagc gcatccagcg gcgctttttg ttgtgcctga atggccaggt
     2461 ctggcagttt ctggaaacct aaattggcat tcgctgcttt tgtcggtaaa tgttcagttt
     2521 ttagctgcac acggggccag tttttcagct ctgagaagag ctcatccacc cgcagccaga
     2581 gcgattgtgg tggcaacagt gggcgcattg ggtcgatgcc gcgattctca aaacgcgcga
     2641 gcgtgtcagc ctggaaacgt tcggcactgt tttccagatc gccagtattc accaacagcg
     2701 tattggcagg gaaataactg aacagtggcg gcagcggttc gctgaagaat agcggctgcc
     2761 agtactcgat cccggcaggt aatgtgcctt tactcacttg ctgataaata tgttccgggt
     2821 cgcgcttcac ttcgaaggta tcacgccact ggctgcggaa cagttcaatt gccgctttat
     2881 cggtcggaaa ttcgtgcgcg ggcaacagat tgatcgcttc tacttcctcc agcgtgcgct
     2941 ggctatcgac gtcaaacacc cgcaggctgt cgatttcatc atcaaagaaa tcaagacgat
     3001 aaggcagctc actccccatc gggaagagat ccaacaacgc gccgcgcgtg gcgtattcgc
     3061 cgtgctccat cacctggtca acatggcgat aaccggcgct gtccagttgg gttcgtaatg
     3121 catctcgtga caggcgctga ccttttttca tcaccagcgc atgaccgtgg agaaagctgt
     3181 gcgggcaaac gcgctgcata agcgtattca ccggaacaat cagtacgcca cgctgcatcg
     3241 atggtaactg gtaaagggtg gaaagacgcg aggagataat gtcctgatga ggcgaaaaac
     3301 tgtcgtaagg gagggtttcc cagtccgcca gattcatcac catctgatcg gtgaactggc
     3361 tgatttcatc atgcaaacgc agagcatttt gcatatctgg tgcaatgagt accaccggac
     3421 cggcgtgacg ttcggcaatt tctgctacca gcgtcgcgca ggcagcgccg gttaactcgc
     3481 ccagcagacg ctgctcaccc gctttgacgg gcagcgtata acgatattgt tcaggcataa
     3541 cggttgtcag attctcgtta ggatatgcct caacatatgg ggcatatctc tgataagcaa
     3601 tgctgttcat tatccgtaat cgacatggtt tagcaaattg aatcgccccg gctggggcga
     3661 tggcttagcg ggagtaactg acggtagcgg gcagagaagg cggggagaac aacagatcgc
     3721 tgaatgcgcg ctgtgaaagt tttcttacca gcaagcccgc aacagtacaa atcaacaaac
     3781 tgacaaaggg gtaaatcagc aggagcgtca gttcgacttg cggcgaccag cgtaccgcgt
     3841 tcatttgcgg aatcaggctt aagctgaata tttctaccag aatgcgatgg gtggtataga
     3901 tagcaatggt gttggagcca atcacattca gcaggctgga ggaacgcata ccgaaacgct
     3961 gctcgtactg ataaaacagc ttcatgatta ccacaatcga taccagcgac aacagcagcg
     4021 agatattaaa caaccaggca ccgaccgcca gaacggtcag caaagaagcc atcagcagat
     4081 ggcggcgcaa cggtaccgct ttaacacagg tcattaatgt tgcgccaaac catgcgccaa
     4141 ggctgtaata aggcaaattg cggatcacac tgttcattcc ccaccacggt gtgggtacga
     4201 aattaacagc cacactcatc agtacaaaca gaacaaataa cggcagggcc agggggttaa
     4261 aaattttaca tatcacgaaa tagacaatta acgcatacag ataccacaag ctggtgctgg
     4321 cggtgatcat cccgtgcagg aactcaccgg tggaatcggc ataagcggca ttggaggcat
     4381 tgcttaaatc gcgctcaggt gccagccact ggttcagcgc acttaacgcc agccactgca
     4441 ccacgcccca aagcgccagc acccagaaga tgttccagat gcgtttatcg agacaatttc
     4501 cccacggtac gctgtcgata tagcggcgaa tcaaatagcc agagataaag aaaaaaaccg
     4561 gcatacgaaa gggtgcaagg taaagattga aatagatcca gcatttgctc aggacttccg
     4621 ataacggatg ctgaaaagtg gtcagatgcg gataaaaggt aatgaccgag tgataaatca
     4681 ccaccagaca aatacataac cctttgatct ggttaatcca tagctctttt tgtttcatcg
     4741 ggacaacacg ctctttttat ttaacatgcg gttgatcctg cggggcgagt cgcgtggtgt
     4801 aatgcgtatc tgtctgtatt tggttatttt tcagataaag atcaggccac atggccgatg
     4861 ataaaatgag gcactttagc cgattgattt gctggtactt ttcagattag ccctgacgat
     4921 cacttacagt tcagacgttt acccatcctg ctttcgctta tatactcgtg tctttgctac
     4981 agcaaccaga cggatttcat gtaccaacct gtcgctctat ttattggcct gcgttacatg
     5041 cgtgggcgtg cagcggatcg cttcggtcgt ttcgtctcct ggctttctac catcggcatt
     5101 accctcgggg tgatggcgct ggtcacagta ttgtcagtga tgaacggctt tgagcgcgag
     5161 ctgcaaaaca acatccttgg cctgatgcca caggcaattc tctcttctga gcatggctct
     5221 cttaacccgc agcaactccc ggaaacggca gtcaaactgg acggcgttaa tcgcgtcgca
     5281 cctattacta ccggtgatgt ggtactgcaa agcgcgcgca gcgtggcggt cggggtgatg
     5341 ctgggtatcg acccggcgca aaaagatcca ctaacgccgt atctggtcaa tgtgaaacaa
     5401 actgacctcg agccggggaa atataatgtc atcctcggtg aacagcttgc ctcacagttg
     5461 ggcgtcaatc gcggtgatca aatccgcgtg atggtgccat ctgctagcca gttcacgccg
     5521 atggggcgta ttccaagtca gcgcctgttc aatgtgattg gcactttcgc cgccaacagt
     5581 gaagtcgatg gctatgaaat gctggtgaat attgaggatg cctcgcgtct gatgcgttat
     5641 ccggcaggca atattaccgg ctggcgtttg tggctggatg agccgctgaa agtcgactca
     5701 ttaagtcagc aaaaactgcc tgaaggcagc aaatggcagg actggcgtga ccgtaaaggc
     5761 gagctgttcc aggccgtacg catggaaaaa aatatgatgg gcttgctgct gagcctgatt
     5821 gtcgccgttg cggcgtttaa cattattacc tcgctgggac tgatggtgat ggagaagcag
     5881 ggcgaagtgg ccattcttca gactcagggg ctgactccgc ggcaaatcat gatggtcttt
     5941 atggtgcaag gggccagcgc cgggattatc ggtgcgatcc tcggagcggc gcttggcgca
     6001 ctgcttgcca gccagttgaa taatctgatg ccgataatcg gcgtcctgct tgatggcgcg
     6061 gcgctgccgg tcgctatcga gcctttacag gtcattgtta ttgcgctggt ggcgatggct
     6121 atcgcgctgc tgtctacgct ttacccttcc tggcgcgctg ccgccactca acccgctgag
     6181 gctttacgtt atgaataaga tcctgttgca atgcgacaac ctgtgcaaac gctatcagga
     6241 aggcagtgtg caaaccgatg tactgcacaa tgtcagtttc agcgtgggcg aaggtgaaat
     6301 gatggcgatc gtcggcagct ctggttccgg taaaagcacc ttgctgcacc tgttaggcgg
     6361 gctggatacg ccaacctccg gcgatgtgat ctttaacggt caaccgatga gcaaactgtc
     6421 ttcggcggcg aaagcagaac tgcgcaacca gaagctggga tttatttatc agtttcacca
     6481 cctgctgccg gattttaccg ccctggaaaa cgtggctatg ccgctgttga ttggcaagaa
     6541 aaagcccgct gaaatcaaca gccgtgcgct ggagatgtta aaagcggtgg ggctggagca
     6601 tcgtgcgaat caccgcccat ctgaactttc tggcggcgaa cgccagcgtg tggcgattgc
     6661 ccgtgcgctg gtcaataacc cgcggctggt actggcggat gaacctactg gtaacctcga
     6721 tgcacgtaac gccgacagca tcttccagtt gcttggggaa ttgaatcgct tgcagggcac
     6781 cgccttcctg gtggttactc acgacctgca actggcgaaa cgtatgagcc gccaactaga
     6841 aatgcgtgat ggacgtctga cggcggaact gagcctgatg ggggcggagt aatggcgatg
     6901 cctttatcgt tattgattgg cctgcgtttt agccgcggac ggcgacgcgg cggcatggtg
     6961 tcgttgatct ccatcatttc taccattggc attgccctcg gcgtggcggt attgatcgtc
     7021 ggcttaagcg cgatgaacgg ctttgaacgc gaactgaata accgcattct ggcggtagta
     7081 ccgcacggtg aaattgaggc ggtagatcag ccgtggacca actggcagga agcactggat
     7141 aacgtgcaga aagtgccggg cattgccgcc gctgcgccgt atatcaattt caccgggctg
     7201 gtggaaagtg gcgcgaatct gcgcgcaatc caggtcaagg gcgttaaccc gcaacaggaa
     7261 cagcgtttga gcgcattacc ctcgtttgtc caggacgatg cgtggagcaa ttttaaagca
     7321 ggcgaacagc aaattatcat cggcaaaggc gtggcggatg cgctgaaagt gaagcagggg
     7381 gattgggtgt cgattatgat cccaaattcg aatcccgagc ataaactgat gcagccaaaa
     7441 cgtgtgcgtt tgcacgttgc cggtattttg cagttgagtg gtcaactcga tcacagtttt
     7501 gccatgatcc cgctggccga tgctcagcaa tatcttgata tgggttccag cgtgtcaggt
     7561 attgccctta aaatgacgga tgttttcaac gccaataagc tggtacgcga tgcgggtgaa
     7621 gtgaccaaca gctatgttta tattaaaagc tggattggta cttacggcta tatgtatcgc
     7681 gatattcaga tgatccgcgc cattatgtat ctggcgatgg tactggtgat tggtgtggcc
     7741 tgtttcaaca tcgtctccac cttagtgatg gcggtgaaag acaagagtgg cgatatcgca
     7801 gtattaagaa cgttgggtgc gaaagatggt ttaatccgcg ccatctttgt ctggtatgga
     7861 ttgctggcag ggctgttcgg cagcctgtgt ggtgtgatta tcggcgtagt tgtttcactg
     7921 caacttaccc cgattattga gtggattgaa aagctgatcg gccatcagtt cctctccagc
     7981 gatatctatt ttattgactt cctgccatcg gaattgcact ggctggacgt cttctacgta
     8041 ctggtcacag cattgttgct gagtcttttg gcaagttggt atccggcgcg gcgcgccagt
     8101 aatattgacc ctgcgcgagt ccttagcggc cagtaaaggc agtacattaa aacaaggagc
     8161 ggcaatgtat tacgggtttg atattggtgg aacaaaaatt gcgctaggcg tgtttgatag
     8221 cggtcggcag ttgcagtggg aaaagcgggt gccgacaccg cgtgacagct atgacgcatt
     8281 tttagatgca gtgtgcgagc tggtagccga agctgaccaa cgttttggct gtaaaggttc
     8341 tgtcggcatt ggtattccgg gtatgccgga aacagaagat ggtacgctgt atgccgccaa
     8401 tgtccctgct gccagcggta aaccgctgcg tgccgacctg agcgcacgtc ttgatcgcga
     8461 tgtacgcctt gataacgatg ccaactgttt tgccctttct gaagcatggg atgatgaatt
     8521 tactcaatat ccactggtga tggggttgat tctcggcacc ggcgttggcg gcgggctgat
     8581 tttcaacggt aaaccaatta ccggtaaaag ctatattacc ggcgagtttg gccatatgcg
     8641 tctgccggtt gatgcgttaa ccatgatggg gctggatttc ccgttacgcc gctgcggctg
     8701 tggtcagcat ggctgcattg aaaattatct gtctggtcgc ggttttgcgt ggctgtatca
     8761 acactattat catcaaccgt tgcaggctcc tgagatcatt gcgctttatg atcaaggcga
     8821 tgagcaggca agggcgcacg ttgagcgtta tctggattta ttagcggttt gtctgggaaa
     8881 tatcctgacc attgttgacc ctgacctggt cgtcattggt ggtggcttat cgaatttccc
     8941 ggcaatcaca acgcaactgg cggaaaggtt gcctcgtcat ctcttacctg tagctcgtgt
     9001 tccgcgcatt gaacgcgcgc gccacggtga tgcgggagga atgcgtggtg cggccttcct
     9061 acatctaacc gattaatcaa cagaggttgc tatgctgtcg cgtcggggtc atcggttaag
     9121 tcgttttcgt aaaaataaac gccgcctgcg cgagcgtttg cgtcagcgta tttttttcag
     9181 agataaagtg gtgccggaag caatggaaaa accaagagta ctcgtactga caggggcagg
     9241 gatttctgcg gaatcaggta ttcgtacctt tcgcgccgca gatggcctgt gggaagaaca
     9301 tcgggttgaa gatgtggcaa cgccggaagg ttttgatcgc gatcctgaac tggtgcaaac
     9361 gttttataac gcccgtcgtc gacagttgca gcagccagaa attcagccta acgctgcaca
     9421 tcttgcgctg gcgaaactgc aagatgctct cggcgatcgc tttttgctgg tgacgcaaaa
     9481 tatcgataac ctgcatgaac gcgcaggcaa taccaatgtg attcatatgc atggggaatt
     9541 gctgaaagta cgttgttcgc aaagtggcca ggtcctcgac tggaccggag acgttacgcc
     9601 agaagataaa tgccattgtt gccagttccc ggcacccttg cgcccgcacg tagtgtggtt
     9661 tggcgaaatg ccactcggca tggatgaaat ttatatggcg ttgtcgatgg ccgatatttt
     9721 cattgccatt ggtacttcgg ggcatgttta tccggcggct gggtttgttc acgaagcgaa
     9781 actgcatggc gcgcacaccg tggaactgaa tcttgaaccg agtcaggttg gtaatgaatt
     9841 tgccgagaaa tattacggtc cggcaagcca ggtggtgcca gagtttgttg aaaagttgct
     9901 gaagggatta taaagcggga aaaattgcct gatgcgctac gcttgtcagg cctgtgaagg
     9961 ggcgcaatta attgaatttg cgggttttgt gggccggata aggcgttcgc gccgcatccg
    10021 gcataataca cgcgaacttt actgcctctt ttaccgttta ctcgtccata gtaacgcggc
    10081 aataaacgta cagacaccaa tcagccctgc ccagagtggc gatattctgg taatcgcaaa
    10141 gctgttatcg atatacaaac ataaagcctg ataacccata ttggcaacag ccatcgccac
    10201 tgcaaatttt aacgcataat aaaggcgcgt tgtgtagtta aaaaatgcag caaagacgat
    10261 gaatgcaact gcccaggcaa aaaggcctaa tgtcgtggta ctgccgggtg gatcattttc
    10321 atgctgttgt ccgggatgcg gataatatgt cagtggtggc gtcggttgtt ttaatatctg
    10381 tgcttgtaaa acagccaatg cattattgat accctgatac cagaggtccg catgtaaata
    10441 agccagcgtg tgattttcta ccagaaggcc gagatgttta tcgtccagac cgctttcaac
    10501 ctggccgttg gttatcataa ttaaccgtgg ctcgcgggta gtaaagagta acagaacact
    10561 tccttttaca ttactcattg aagatgccgc atgcgccagc atcgctttgc cattttgcct
    10621 gagactggct tcattattaa tgtcgggtaa aaccacaata aacggtgcaa ctccggtttg
    10681 ttggcgaaaa agctcggctt tatgcgcgat ctcttcagca tctggtacag taccggtaac
    10741 atccgtaaaa agtgtcttca ttattggaat taagggctta ctggttgccg tcagaggcag
    10801 gagtatcagt aaactgagca atattatgat ttttttcatg ggcgggactt ccgtttcctg
    10861 cgtgtctttt tagaggaggg catactgatt aataaaccga cggagataaa aataacgccc
    10921 ataatgccga taaaccccca tttaaaaaaa ttaacccacg ggttatttat ctcagccttt
    10981 tcaggatgtc ctggagaata ataaacatta actttatcgc cttcgctgcc ttcataaaaa
    11041 tcgctgccaa tactcgaatt gaaaattagc gtgtaatctg gcgtcggacg aaaggcaacg
    11101 acgggatacc acgaaccatt tttacccgtg cgatgagaat gactggagtg ccagacggta
    11161 tcaacaatga cgccttcagt atgaatcgcg tctttgctat aggtgaactg gttataaaag
    11221 ataacaacag aaatgaggat cagtaccaga ccgataccga agaatatacg aaagaaaact
    11281 ctactgtcct ggctcattag ccacatcctt gctaaataaa aaacgggcgg taatatcacc
    11341 gcccgcttgc tgaattaacg tcctgctttc agcttctgat aatactcttc ataaatgctg
    11401 ctggctgcgc caacgtcatt ctgccattca ccatttttaa tggtttcagc atccgggtag
    11461 agcgttttat cgttcgccac ttccggactt aacagcttac gcgccgcaag gtttggcgtt
    11521 ggataaccga tagtttcagc aacctgtttt gccacatccg ggcgcagcag gaagttgatc
    11581 aatttcagcg cgccttcttt gtttttggca tttgccggga tcgccaggct gtccatccag
    11641 aaaataccgc cttctttcgg ccacaccaca tcaattggcg tacccgcctg gcgtgcaacg
    11701 aaagcagaac cgttccagat catgccgagg ttaacttcgc cttccatata cgggttcgcc
    11761 gggttatcgg agttaaacgc cgcgacgttt ggcatcagct ttttcagctc gttgtatgca
    11821 gcttcaatct ctttcggatc ggtggtgtta ccggagtagc ccagcttacg cagcgccatc
    11881 tggaacactt cacgggcatc gtcggtcagc agcaggctgc ctttgtactc aggcttccac
    11941 agatcggccc agctggtgac agatttcgga tccaccgcat caccgttaac gccaatcgcc
    12001 gtcgcacccc agatatacgg aatggaatag tcgttattcg ggtcaaacgg cttgttgagc
    12061 atgtctggat cgaggttgct gaaattgctt aacttcgact tgtcgatctt ctggatcatc
    12121 ccttctttac gcattttatc gacgtaatag gttgaaggaa ccaccaggtc ataggcaccg
    12181 tctttgtacg ttttcagctt cgcgtacatg gtttcgttcg actcgtaagt cgaatagata
    12241 accttaatac cggtttcttt ggtgaactgt tcaagcagtc ctggcggcac gtactcggtc
    12301 cagttgtaga aatacagcgt gttgttgtca tcggcgtgag cggcgctcat gcccagtgcc
    12361 agagcacccg ccgcgagcag gtggcgtgac cattttttca tttaacgtcc cctgtgttac
    12421 ctttcgtttt atcacgagca ataagctggc tggcaattac catcaccagc gacagcacca
    12481 gtaatatggt tgccagcgcg ttaacttccg gcgatacgcc gactttgacc atcgaataaa
    12541 tttttaacgg cagaatttca taactcggcc cggtgacaaa cgaagaaacc accacgtcgt
    12601 ccatcgacag ggtaaagctt aacacccagc ccgccgctac cgctggcatt gccagtggca
    12661 gaatgatttt tcgcagaatg gtaaattcgc tggcaccgag atctttcgcc gcttccagca
    12721 ttcgcacgtc aaaacctttc aggcgcgaat acactgtcac taccacaaat ggcaggcaga
    12781 aggtgatatg cgagaacagc agcgaccaga agccaagctg aatacccagc agcataaaca
    12841 gcaccagcag cgaaatcgcc attacgatat ctggtgacat catcaccaca aacagcattc
    12901 cgctaacgaa cggcttacca cgaaagcgat aacggtacag cgcaactgcc gtcagtgaac
    12961 cgataagcgt agcaaacgtc gccgaaaaca ccgccattgt aagtgaatgc tgcgctgcct
    13021 gtaacaggct gtcgttgttc atcagcaggc tataccattt ggtggtaaaa ccctgccagt
    13081 tgatgccaaa gcgcgagctg ttaaaggagt tcacaatcaa aataatgatt gggatataca
    13141 ggtacgcgta gatagcggtc ataaaaccgc cgcgaagcag tcgaccgatc attcgagttc
    13201 caccttctta ttcataaggt ggagcacttt caggattttg aatttattca attttttcct
    13261 ttctccaaaa tctctccaaa acgtttcccc aaaactaggc cacgcatttt acaacacttt
    13321 tttcctgaga tggtgacgga tattccatat ggtgtttgtc aaataaccaa cttaacccat
    13381 tcctgacctc gagtatcgtt atagcgatcg gtggttgcct ggactttatg tcctagtaat
    13441 gtttttgtat cgataccctg tgcacggtac agccgttctg atagagagcg ttgttcatga
    13501 aatgttggcg gagtttttcc tgctggtgga attatcccag ccagatcccg tgctttggca
    13561 aagtagtcgc tcaggttgtc tttactcatc ggcttcggtt gtttctggtg ccgactatgg
    13621 attagatatg gacttaatat tctgtctcgg cacccatcaa taacttcttt taacgttatc
    13681 ccaatggcat cacagcgtag tgtaagcggt aacgccagac gcattccggt ttttccctgg
    13741 gtgatatgca agtgttcgtt ccacacatct gaaaaacgca tgtggcaaat gtcatcacgg
    13801 cgctgaccag taacaatcgc aagaagcatt gcgttacgga taaagtgttt ttcaggcgtt
    13861 gcattgtaaa ttttttgcca gtcttccatg gtgagcctgg ctctggttac tttagggatc
    13921 ggtttacggg tagcctccgg aggattccat ccaggaggaa cttcccctgc atgctgtgct
    13981 tctttataaa tatcaaccca taatccacga tttactctcg ctgtgctgac catgtcttta
    14041 tccagccact catccagtat taatgcaaag tctcttactt ccagttcttt caatgggtgg
    14101 tttcccagac gggaaaccag gtatgcagcc attcgagttt tttctttgtg agttgtagct
    14161 gcaatatctc catttttcag tcgcgtgtcc tgtattttca gatatcgatc aacccatgcc
    14221 tttaatctga taccccgacg ttttgttgct gacggacttt catcaatttt gcgcatgaaa
    14281 tattcagctt ctgctgcagc tattcgctga ttggctgtgg aagcgatttt ttctgcctta
    14341 cctttgtctg ttccgagtcc gtgaaatttt ccagtcacag gatttttata ctggtagtaa
    14401 actctgccag ttctgcgatc aaacttttcg taaagaccgg ctacgtcagt gctgtttttt
    14461 cgtggcctcg gtgacatgag ttaaaatctc cttcagtgca tcatcatcgc cagtatgaat
    14521 ttccggcgca attcccgttt caccaggccc aacaaatact gctcggcgat ctatcagcca
    14581 acgcccacga attttttgtg gtcttggaac gatgtatcct agttttccgt atttcaccag
    14641 ggtagtgttt gttattggga gactgaaccg ttttggtttc cactcgtcga gcgttatcag
    14701 gtactgttcg ctcatggcta tcactccgga acgcgccagt tgcagaatat caacgacaac
    14761 tggcgacggt tgaacattaa aaatcagcct gactcgggat cagtttttgc cagataactg
    14821 aaacgtattt tgcctggtaa cgggcgtcat caagtgcatt atggcgctca ccttcgaatg
    14881 gaatagccgt tctggcatcg aagtctatgg ctttccccag ctcaacgatt gtgcgtacat
    14941 cgcgatcgtt gtagtaacgc cacgggcagg ggatcccctg ccgttcgtat gaacggcgca
    15001 aaatcgtgtt gtcgaagttg gctccatttc cccagacctg aacaaaaaat tcgccggagt
    15061 tttcgtcgat aaattcccgc aattgtaaca gtgcatcatc taacgggatt tcatcggtca
    15121 taatggcaga ttgcgcttcg cgtgattgct taagccacca tttaatggtg tcccgatcaa
    15181 tgactccgcc agcagtttcc agatcgatag tcttactaaa ttccggtccc atatctccgg
    15241 tttgcggatc gaaaaatatt gcacctattg agataatcgg ggcatctgga ttttttccca
    15301 tggtttcaag gtcgatcatt agatggtcac acgtcccgct ggtggatgtg cctttatgat
    15361 gaccgttcac cttaactgag tgatctgtcg cctcgccagt ttcactatcg ctatcgtgat
    15421 gctgattgcc gccagggttc tccttgtgtg gatgttcagt gccttccatt tcctccggat
    15481 cattttcctg aacttcaacc tgattctctt cattgaatgt ttcctggtat gttgcgtcgc
    15541 ccatcaccgc gccacaatca gggcagttgc cgccgccggt ctgaccgcag gcggtgcaga
    15601 tcttttccgg ttcctgttgc actactggtt caggttgttt cgtttctggc tcgttttgta
    15661 acgcatttgg gctgttttgt tccgctttct ggccgttctg ttccgtttct tgctggttct
    15721 ggttcacaga atcgcgggtt tccatcccct tgacccattt cggatcattc gggtcgctaa
    15781 tccctgcaac aaattctccg cgagaggccg caagcaactt atcggcgtca ggctggctga
    15841 tattggctgc ctgcataatt ttgtttactt cgtcagcggt aacttttacc tgattaaccg
    15901 gatctatctg tgcctgagta tccagcgatt gcgtgtcctg gcgatgttca gttgtattcg
    15961 gctccattgc ttcagtcgtt gcctgttcat ctgccattgc gtcagacggt tgtgattttt
    16021 cctcattatt tttttcttct tctgtttcgc gttcagcagc cagttcgcgg ttaacttctt
    16081 ccaggatatc tttttccggt gtatgtcgtg cagcagcgag agtttcctcg gtagggttct
    16141 cgtgatcagt ttccgtcaaa taggcgttga tatacccctg aaggcgtccc gggtagtgat
    16201 aaaattcagg gtgtgcgctt cggataagtg caaaaatagc ggcgcgggaa taatccagga
    16261 tacccgggat tgcgcgaagt gctgcggacc attctttgaa cggactttct ttcttccgga
    16321 cgacttcttt tgcgcgacga taaacgctgc ccggaatttc ataaatatta aaatccatcg
    16381 gaagtgtggc cgctgcaatc tccacatcca gcgtatcgaa ggtgtgtact aaattcggat
    16441 tgcgatcggt tttgttccct ccaccggcat tagcaccgga agccgtgcgg gtgatgcgtg
    16501 aaacacgatt tcctttcatc cactcttttg tcagcagacc gcgatcggtg tagtcagcgc
    16561 ccaggtatgc ttcgaaaaaa gcagtcatca gtcccaggtc tgaattacca ggattaggga
    16621 aaactttgtc cgtgtcacga accagtttgt ggagatcgcg aatttccagc ggttcgagtt
    16681 gcgctgcttt atttgagatg gccagcgcgg taacggcggg aagtttttca tcctgtgcgt
    16741 tatgtaatgt gcgcagttcg tcccgcgaaa cgtgtgtgat cggtttttcg ctgccgtgct
    16801 gtgccagcca tcgaacaggc agaatctgac ctgaaaccgg caggagcatg ttctcctcaa
    16861 tctcagccat gttttcgccg ttgacgttgg tattgtcagt gctggctggt ttatcctgaa
    16921 cagagggcga gggcgcgata agtaccattg tgataccatc ttcaccgccc ttttcgtatc
    16981 ggttgcagaa ttcggtatca aacacgcctt ccggaggaag gtcattcaca acgggtaaat
    17041 ggacgcgaac aggtttttta aaatcctctt tgtcaaatcc ggcatcgtcc attgcgacaa
    17101 caccgcgtga tattgcaacc gataattttt ttgctgtgcg ccagtaaaaa ccgcctttaa
    17161 ttccaagacg ttttctgatt ttgtcatttt tggcttcgta atatagtgca atttcttctt
    17221 tatcagtgct cattgataaa cctcattaca gatttaaggg tgaacaaatc cctgccattg
    17281 ctggcatata aaaatgaaac cggatattaa ttacggtgct gttttaagcc ccgccgggat
    17341 ttcgttattg tccatgtgaa taactttatc gaccggataa cagttgccgg gaattttctg
    17401 ttccgctgcg gcagccatgc attctttcat tgagttgtac atgccggtga ttgcatcaag
    17461 cgactcacca gtattgagat gtacagtcag aatgagtacg aataatgtgt tcatcgccac
    17521 tctccgaaaa taccgagttt aagaagggca attctggaga gtatggaatt gtcattcagc
    17581 aggtagggtt catattttct catattgatt gcatcctcgg tgaaatccct gttactgagc
    17641 agaacaccaa tattaaagca cccgtcagac gtattaacgt ttggtagtga cgtttccatt
    17701 atcgcgtcct caacaatgaa tttttagcaa ttgctccaca gtcatatttt taattgcgct
    17761 ccggtttaca agagtccatc cttgtttctc cagataaaac cggaaggtat ccagggtgca
    17821 gaccattgcg ccgtcaggaa cggtttcagt gaatttgata ttgccgtgtt cgtcgagacg
    17881 gataaccagg gtgcgtccgt ccccgtgaat cattttgtcg ggagacgggg cgttattctg
    17941 gcgcagttct tcctccatgc gatcgaactc agcgatgtag gcttctttga aagttgcggc
    18001 ttttttacct gtgaacccca tcaccaggaa aacgaagccg tttttggtga tttggtacat
    18061 ggggcgtttt tcgcctttgg cgtcggtgta ggtaacgggc gtaaaattgc gctcgttaaa
    18121 atcttcagag cattctagcg tttcaatttt gcgcagaacg tccttgtgca ttttgcggaa
    18181 gaactctgca accgcaacag acgtagtgac agcgcgacca ttttcgatgg ttacgtcagg
    18241 gtgagaaagg gtagggatag tagccataat ggcagcctct tggttgagta ttaataactc
    18301 accaccaagg ttttccacga ccataagggt ggtgaggcgt acaggggtgg aaataccggt
    18361 caaccaagaa tccggccagc cttgcagctg ccctgcacgc cccaccataa tctgaatgtg
    18421 gctgtgcttg acgcataaaa aaaccgcctg agcgcggtta tgcgcttgat tgaattccgg
    18481 gtttccacgc ccggcacccg ttttatgagg tgcaggtgca ctataattcc acccgttctg
    18541 gttttcaata gctacattca acattttctc ttacctttca tcaccgaagt gaactttgtt
    18601 gatgcggtgc ctggtgcctc caggtgacgt taaccagtta acaattaacg ccggatacag
    18661 agaatccacc cataacactg tttttggttt taactgttcc gcgtgcgctt agccgcattc
    18721 accgcatcac aaaattcact ttaaaaaggg tggcagagca gtcacggagt agaactgatg
    18781 ccaccaaaga ctacacatgg gtattgtggc ggggctgtca cttaagcgta tggtcaacct
    18841 gacaacccgg tgcattttct ggagcaatgg aggaaacccc agccatactt accgccgcgc
    18901 catttcgcgg attgccacaa ccggaagcgc acgatcgaat taaatttaac gacgacctat
    18961 acagagagac taacttcgcc gggcgctttc gtgttatgcc ctgacttttc agggatatat
    19021 cctttttagt aaactgtcag tgccggattc ttatccgtgt ccggcgcacg cactctacct
    19081 cacctgtgaa taaattaatg attaattgat attttgttgt ttgattcaac tttcccatcg
    19141 gatgtgtgat gctttaaatc acaggaatta atactgctcg ctgtaaaatg attttcaagg
    19201 ggagctattc gaatcccttt ctttttcatt aacaagccaa atccttta
//
LOCUS       DAHHTG010000083        19024 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.083, whole genome shotgun sequence.
ACCESSION   DAHHTG010000083 DAHHTG010000000
VERSION     DAHHTG010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19024)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19024)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19024
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.083"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            complement(<1..92)
                     /locus_tag="NBI74_003004"
     rRNA            complement(<1..92)
                     /locus_tag="NBI74_003004"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
     gene            573..1265
                     /locus_tag="NBI74_003005"
     CDS             573..1265
                     /locus_tag="NBI74_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HCD1933297.1"
                     /translation="MPLSAQQLAAQKNLSYVLAEKLAQRILKGEYEPGTILPGEIELG
                     EQFGVSRTAVREAVKTLTAKGMVLPRPRIGTRVMPQSNWNFLDQELLTWWMTEENFHQ
                     VIDHFLVMRICLEPQACLLAATVGTAEQKAHLNTLMAEMAALKENFRRERWIEVDMAW
                     HEHIYEMSANPFLTSFASLFHSVYHTYFTSITSDTVIKLDLHQAIVDAIIQSDGDAAF
                     KACQALLRSPDK"
     gene            1288..2715
                     /gene="mdtD"
                     /locus_tag="NBI74_003006"
     CDS             1288..2715
                     /gene="mdtD"
                     /locus_tag="NBI74_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter subunit MdtD"
                     /protein_id="HCD1933298.1"
                     /translation="MSDKKKRSMAGLPWIAAMAFFMQALDATILNTALPAIAHSLNRS
                     PLAMQSAIISYTLTVAMLIPVSGWLADRFGTRRIFTLAVSLFTLGSLACALSNSLPQL
                     VVFRVIQGIGGAMMMPVARLALLRAYPRNELLPVLNFVAMPGLVGPILGPVLGGVLVT
                     WATWHWIFLINIPIGIAGLLYARKHMPNFTTARRRFDTTGFLLFGLSLVLFSSGIELF
                     GEKIVASWIALTVIVTSIWLLLLYILHARRTPNPLISLDLFKTRTFSIGIVGNIATRL
                     GTGCVPFLMPLMLQVGFGYQAFIAGCMMAPTALGSIIAKSMVTQVLRRLGYRHTLVGI
                     TVIIGLMIAQFSLQSPAMAIWMLILPLFILGMAMSTQFTAMNTITLADLTDDNASSGN
                     SVLAVTQQLSISLGVAVSAAVLRVYEGMEGTTTVEQFHYTFITMGIITVASAAMFMLL
                     KTTDGNNLIKRQRKSKPNHVPSESE"
     gene            complement(2681..3673)
                     /gene="rbsR"
                     /locus_tag="NBI74_003007"
     CDS             complement(2681..3673)
                     /gene="rbsR"
                     /locus_tag="NBI74_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose operon transcriptional repressor RbsR"
                     /protein_id="HCD1933299.1"
                     /translation="MATMKDVARLAGVSTSTVSHVINKDRFVSEAITAKVEAAIKELN
                     YAPSALARSLKLNQTHTIGMLITASTNPFYSELVRGVERSCFERGYSLVLCNTEGDEQ
                     RMNRNLETLMQKRVDGLLLLCTETHQPSREIMQRYPTVPTVMMDWAPFDGDSDLIQDN
                     SLLGGDLATQYLIDKGHTRIACITGPLDKTPARLRLEGYRAAMKRAGLNIPDGYEVTG
                     DFEFNGGFDAMRQLLSHPLRPQAVFTGNDAMAVGVYQALYQAELQVPQDIAVIGYDDI
                     ELASFMTPPLTTIHQPKDELGELAIDVLIHRITQPTLQQQRLQLTPILMERGSA"
     gene            complement(3677..4606)
                     /gene="rbsK"
                     /locus_tag="NBI74_003008"
     CDS             complement(3677..4606)
                     /gene="rbsK"
                     /locus_tag="NBI74_003008"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HCD1933300.1"
                     /translation="MQNAGSLVVLGSINADHILNLQSFPTPGETVTGNHYQVAFGGKG
                     ANQAVAAGRSGANIAFIACTGDDSIGESVRQQLSTDNIDISPVSVIKGESTGVALIFV
                     NGEGENVIGIHAGANAALSPALVEAQRERIANASALLMQLESPLESVMAAAKIAHQNK
                     TIVALNPAPARELPDELLALVDIITPNETEAEKLTGIRVENDEDAAKAAQVLHEKGIR
                     TVLITLGSRGVWASVNGEGQRVPGFRVPAVDTIAAGDTFNGALITALLEEKPLPEAIR
                     FAHAAAAIAVTRKGAQPSVPWREEIDAFLDKQR"
     gene            complement(4732..5622)
                     /gene="rbsB"
                     /locus_tag="NBI74_003009"
     CDS             complement(4732..5622)
                     /gene="rbsB"
                     /locus_tag="NBI74_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter substrate-binding protein
                     RbsB"
                     /protein_id="HCD1933301.1"
                     /translation="MNMKKLATLVSAVALSATVSANAMAKDTIALVVSTLNNPFFVSL
                     KDGAQKEADKLGYNLVVLDSQNNPAKELANVQDLTVRGTKILLINPTDSDAVGNAVKM
                     ANQANIPVITLDRQATKGEVVSHIASDNVLGGKIAGDYIAKKAGEGAKVIELQGIAGT
                     SAARERGEGFQQAVAAHKFNVLASQPADFDRTKGLNVMQNLLTAHPDVQAVFAQNDEM
                     ALGALRALQTAGKSDVMVVGFDGTPDGEKAVNDGKLAATIAQLPDQIGAKGVETADKV
                     LKGEKVQAKYPVDLKLVVKQ"
     gene            complement(5647..6612)
                     /gene="rbsC"
                     /locus_tag="NBI74_003010"
     CDS             complement(5647..6612)
                     /gene="rbsC"
                     /locus_tag="NBI74_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002882531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter permease"
                     /protein_id="HCD1933302.1"
                     /translation="MTTQTVSGRRYFTKAWLMEQKSLIALLVLIAIVSTLSPNFFTIN
                     NLFNILQQTSVNAIMAVGMTLVILTSGIDLSVGSLLALTGAVAASIVGIEVNALVAVA
                     AALALGAAIGAVTGVIVAKGRVQAFIATLVMMLLLRGVTMVYTNGSPVNTGFTENADL
                     FGWFGIGRPLGVPTPVWIMGIVFLAAWYMLHHTRLGRYIYALGGNEAATRLSGINVNK
                     IKIIVYSLCGLLASLAGIIEVARLSSAQPTAGTGYELDAIAAVVLGGTSLAGGKGRIV
                     GTLIGALILGFLNNGLNLLGVSSYYQMIVKAVVILLAVLVDNKKQ"
     gene            complement(6617..8122)
                     /gene="rbsA"
                     /locus_tag="NBI74_003011"
     CDS             complement(6617..8122)
                     /gene="rbsA"
                     /locus_tag="NBI74_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter ATP-binding protein RbsA"
                     /protein_id="HCD1933303.1"
                     /translation="MEALLQLKGIDKAFPGVKALSGAALNVYPGRVMALVGENGAGKS
                     TMMKVLTGIYTRDAGTLLWLGKETTFTGPKSSQEAGIGIIHQELNLIPQLTIAENIFL
                     GREFVNRFGKIDWKTMYAEADKLLAKLNLRFKSDKLVGDLSIGDQQMVEIAKVLSFES
                     KVIIMDEPTDALTDTETESLFRVIRELKSQGRGIVYISHRMKEIFEICDDVTVFRDGQ
                     FIAEREVASLTEDSLIEMMVGRKLEDQYPHLDKAPGDIRLKVDNLCGPGVNDVSFTLR
                     KGEILGVSGLMGAGRTELMKVLYGALPRTSGYVTLDGHEVVTRSPQDGLANGIVYISE
                     DRKRDGLVLGMSVKENMSLTALRYFSRAGGSLKHADEQQAVSDFIRLFNVKTPSMEQA
                     IGLLSGGNQQKVAIARGLMTRPKVLILDEPTRGVDVGAKKEIYQLINQFKADGLSIIL
                     VSSEMPEVLGMSDRIIVMHEGHLSGEFTREQATQEVLMAAAVGKLNRVNQE"
     gene            complement(8130..8549)
                     /gene="rbsD"
                     /locus_tag="NBI74_003012"
     CDS             complement(8130..8549)
                     /gene="rbsD"
                     /locus_tag="NBI74_003012"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709562.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HCD1933304.1"
                     /translation="MKKGTVLNSDISSVISRLGHTDTLVVCDAGLPIPKSTTRIDMAL
                     TQGVPSFMQVLGVVTNEMQVEAVIIAEEIKQHNPQLHETLLTHLEQLQQHQGNTIEIR
                     YTTHEQFKQQTAESQAVIRSGECSPYANIILCAGVTF"
     gene            complement(8716..10584)
                     /gene="kup"
                     /locus_tag="NBI74_003013"
     CDS             complement(8716..10584)
                     /gene="kup"
                     /locus_tag="NBI74_003013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity potassium transporter Kup"
                     /protein_id="HCD1933305.1"
                     /translation="MSTDNKQSLPAITLAAIGVVYGDIGTSPLYTLRECLSGQFGFGV
                     ERDAVFGFLSLIFWLLIFVVSIKYLTFVMRADNAGEGGILTLMSLAGRNTSARTTSML
                     VIMGLIGGSFFYGEVVITPAISVMSAIEGLEIVAPQLDTWIVPLSIIVLTLLFMIQKH
                     GTAMVGKLFAPIMLTWFLILAGLGLRSIIANPEVLHALNPMWAVHFFLEYKTVSFIAL
                     GAVVLSITGVEALYADMGHFGKFPIRLAWFTVVLPSLTLNYFGQGALLLKNPEAIKNP
                     FFLLAPDWALIPLLIIAALATVIASQAVISGVFSLTRQAVRLGYLSPMRIIHTSEMES
                     GQIYIPFVNWMLYVAVVIVIVSFEHSSNLAAAYGIAVTGTMVLTSILSTTVARQNWHW
                     NKYFVALILIAFLCVDIPLFTANLDKLLSGGWLPLSLGTVMFIVMTTWKSERFRLLRR
                     MHEHGNSLEAMIASLEKSPPVRVPGTAVYMSRAINVIPFALMHNLKHNKVLHERVILL
                     TLRTEDAPYVHNVRRVQIEQLSPTFWRVVASYGWRETPNVEEVFHRCGLEGLSCRMME
                     TSFFMSHESLILGKRPWYLRLRGKLYLLLQRNALRAPDQFEIPPNRVIELGTQVEI"
     gene            10807..12303
                     /gene="ravA"
                     /locus_tag="NBI74_003014"
     CDS             10807..12303
                     /gene="ravA"
                     /locus_tag="NBI74_003014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312715.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA"
                     /protein_id="HCD1933306.1"
                     /translation="MAHPHLLAERISRLSSSLEKGLYERSHAIRLCLLAALSGESVFL
                     LGPPGIAKSLIARRLKFAFQNARAFEYLMTRFSTPEEVFGPLSIQALKDEGRYERLTS
                     GYLPEAEIVFLDEIWKAGPAILNTLLTAINERQFRNGALVEKIPMRLLVAASNELPEA
                     DSSLEALYDRMLIRLWLDKVQDKANFRSMLTSQQDENDNPVPASLQITDEEYERWQKE
                     IGEITLPDHVFELIFMLRQQLDKLPDAPYVSDRRWKKAIRLLQASAFFSGRSAVAPVD
                     LILLKDCLWYDAQSLNLIQQQIDVLMTGHAWQQQGMLTRLGAIVQRHLQLQQQQSDKT
                     ALTVIRLGGIFSRRQQYQLPVNVTASTLTLLLQKPLKLHDMEVVHISFERSALEQWLS
                     KGGEIRGKLNGIGFAQKLNLEVDSAQHLVVRDVSLQGSTLALPGSSAEGLPSEIKQQL
                     EELESDWHKQHVLFSEQQKCLFIPGDWLGRIEASLQDVGAQIRQAQQC"
     gene            12297..13748
                     /gene="viaA"
                     /locus_tag="NBI74_003015"
     CDS             12297..13748
                     /gene="viaA"
                     /locus_tag="NBI74_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA stimulator ViaA"
                     /protein_id="HCD1933307.1"
                     /translation="MLTLDTLNVMLAVSEEGLIEEMIIALLASPQLAVFFEKFPRLKA
                     AITDDVPRWREALRSRLKDARVPPELTEEVMCYQQSQLLSTPQFIVQLPQILDLLHRL
                     NSPWAEQARQLVDANSTITSALHTLFLQRWRLSLIVQATTLNQQLLEEEREQLLSEVQ
                     ERMTLSGQLEPILADNNTAAGRLWDMSAGQLKRSDYQLIVKYGEFLNEQPELKRLAEQ
                     LGRSREAKSIPRNDAQMETFRTMVREPATVPEQVDGLQQSDDILRLLPPELATLGITE
                     LEYEFYRRLVEKQLLTYRLHGESWREKMIERPVVHKDYDEQPRGPFIVCVDTSGSMGG
                     FNEQCAKAFCLALMRIALAENRRCYIMLFSTEIVRYELSGPQGIEQAIRFLSQQFRGG
                     TDLASCFRAIMERLQSREWFDADAVVISDFIAQRLPDDVTSKVKELQRVHQHRFHAVA
                     MSAHGKPGIMRIFDHIWRFDTGMRSRLLRRWRR"
     gene            complement(13753..14745)
                     /gene="asnA"
                     /locus_tag="NBI74_003016"
     CDS             complement(13753..14745)
                     /gene="asnA"
                     /locus_tag="NBI74_003016"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HCD1933308.1"
                     /translation="MKTAYIAKQRQISFVKSHFSRQLEERLGLIEVQAPILSRVGDGT
                     QDNLSGCEKAVQVKVKALPDAQFEVVHSLAKWKRQTLGQHDFSAGEGLYTHMKALRPD
                     EDRLSPLHSVYVDQWDWERVMGDGERQFSTLKSTVEAIWAGIKATEAAVSEEFGLAPF
                     LPDQIHFVHSQELLSRYPDLDAKGRERAIAKDLGAVFLVGIGGKLSDGHRHDVRAPDY
                     DDWSTPSELGHAGLNGDILVWNPVLEDAFELSSMGIRVDADTLKHQLALTGDEDRLEL
                     EWHQALLRGEMPQTIGGGIGQSRLTMLLLQLPHIGQVQCGVWPAAVRESVPSLL"
     gene            14897..15355
                     /gene="asnC"
                     /locus_tag="NBI74_003017"
     CDS             14897..15355
                     /gene="asnC"
                     /locus_tag="NBI74_003017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator AsnC"
                     /protein_id="HCD1933309.1"
                     /translation="MENYLIDNLDRGILEALMGNARTAYAELAKQFGVSPGTIHVRVE
                     KMKQAGIITGARIDVSPKQLGYDVGCFIGIILKSAKDYPSALAKLESLDEVTEAYYTT
                     GHYSIFIKVMCRSIDALQHVLINKIQTIDEIQSTETLIVLQNPIMRTIKP"
     gene            15445..15888
                     /gene="mioC"
                     /locus_tag="NBI74_003018"
     CDS             15445..15888
                     /gene="mioC"
                     /locus_tag="NBI74_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein MioC"
                     /protein_id="HCD1933310.1"
                     /translation="MADITLISGSTLGGAEYVAEHLAEKLEEAGFTTETLHGPLLEDL
                     SASGIWLVISSTHGAGDIPDNLSPFYEALQEQKPDLSAVRFGAIGIGSREYDTFCGAI
                     DKLEAELKNSGAKQTGETLKINILDHDIPEDPAEEWLGSWINLLK"
     gene            16267..18156
                     /gene="mnmG"
                     /locus_tag="NBI74_003019"
     CDS             16267..18156
                     /gene="mnmG"
                     /locus_tag="NBI74_003019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HCD1933311.1"
                     /translation="MFYPDPFDVIIIGGGHAGTEAAMAAARMGQQTLLLTHNIDTLGQ
                     MSCNPAIGGIGKGHLVKEVDALGGLMAKAIDQAGIQFRILNASKGPAVRATRAQADRV
                     LYRQAVRTALENQPNLMIFQQAVEDLIVENDRVVGAVTQMGLKFRAKAVVLTVGTFLD
                     GKIHIGLDNYSGGRAGDPPSIPLSRRLRELPLRVGRLKTGTPPRIDARTIDFSVLAQQ
                     HGDNPMPVFSFMGNASQHPQQVPCYITHTNEKTHDVIRSNLDRSPMYAGVIEGVGPRY
                     CPSIEDKVMRFADRNQHQIFLEPEGLTSNEIYPNGISTSLPFDVQMQIVRSMQGMENA
                     KIVRPGYAIEYDFFDPRDLKPTLESKFIQGLFFAGQINGTTGYEEAAAQGLLAGLNAA
                     RLSADKEGWAPARSQAYLGVLVDDLCTLGTKEPYRMFTSRAEYRLMLREDNADLRLTE
                     IGRELGLVDDERWARFNEKLENIERERQRLKSTWVTPSAEAAAEVNAHLTAPLSREAS
                     GEDLLRRPEMTYEKLTTLTPFAPALTDEQAAEQVEIQVKYEGYIARQQDEIEKQLRNE
                     NTLLPATLDYRQVSGLSNEVIAKLNDHKPASIGQASRISGVTPAAISILLVWLKKQGM
                     LRRSA"
     gene            18220..18843
                     /gene="rsmG"
                     /locus_tag="NBI74_003020"
     CDS             18220..18843
                     /gene="rsmG"
                     /locus_tag="NBI74_003020"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HCD1933312.1"
                     /translation="MLNKLSLLLKDAGISLTDHQKNQLIAYVNMLHKWNKAYNLTSVR
                     DPNEMLVRHILDSIVVAPYLQGERFIDVGTGPGLPGIPLSIVRPEAHFTLLDSLGKRV
                     RFLRQVQHELKLENIEPVQSRVEEFPSEPPFDGVISRAFASLNDMVSWCHHLPGEQGR
                     FYALKGQMPEDEIALLPEEYQVESVVKLQVPALDGERHLVVIKANKI"
BASE COUNT         4761 a         4939 c         4702 g         4622 t
ORIGIN      
        1 aaacagcaag ctgtttcctg ttaccgttcg acttgcatgt gttaggcctg ccgccagcgt
       61 tcaatctgag ccatgatcaa actcttcaat ttaaaagttt gatgctcaaa gaattaaact
      121 tcgtaatgaa ttacgtgttc actcttgaga cttggtattc atttttcgtc ttgcgacgtc
      181 aagaatccgt atcttcgagt gcccacacag attgtctgat aaattgttaa agagcagtgc
      241 cgcttcgttt tttcttagcg gcgcggggtg tgcataatac gctctcccgc tacagagtca
      301 agcatttatt tttgctttct ctgccggaat tctcaggaga atcccgctga cccggcggcg
      361 tgtttgccgt tgttccgtgt cagtggtggc gcattatagg gagttattcc ggcctgacaa
      421 gaggaaattt aaaataattt tctgaccgcg cattttttca gcaaaagccc cgttaatgtg
      481 ccataaaaca agcaatttgc cgtttttgca accgtaatca cacttcctgg tggcatacta
      541 aggaggaata aaaagaaaaa ggaagattag taatgccttt aagcgcacaa cagttagctg
      601 cacagaaaaa cctttcgtat gttctggctg aaaagctggc gcagcggatc ttaaaaggtg
      661 aatatgaacc cggcaccatt ttgcctggtg agattgagct gggcgagcaa tttggagtga
      721 gtcgtacagc ggtacgcgaa gcggtcaaaa cgttaacggc aaaagggatg gttttaccgc
      781 gaccgcgaat tggtacccga gtcatgccac aatcgaactg gaattttctt gatcaggaat
      841 tgcttacctg gtggatgaca gaagagaact ttcatcaggt catcgatcac tttctggtta
      901 tgcgtatctg tctggagccg caagcttgcc tactggcagc aacggttggc accgcggaac
      961 agaaggcgca tctcaatacg ttaatggccg aaatggcggc attaaaagag aactttcgcc
     1021 gtgaacgctg gatcgaagtc gatatggcct ggcatgagca tatctatgaa atgagcgcca
     1081 atccgttttt gacctcattc gcctcgctat tccattcggt ttatcacact tacttcacgt
     1141 caattaccag cgacacagtg ataaagctgg atctgcacca ggcgattgtc gatgcgatta
     1201 tccaaagcga tggcgacgcg gcatttaaag cttgtcaggc gctgctacgc tcaccagata
     1261 agtgataacc cgataacagg actttgaatg agcgataaaa agaagcgcag tatggcgggt
     1321 ttgccgtgga tcgcggcgat ggccttcttc atgcaggcac ttgatgccac tattctgaat
     1381 accgccttac ccgcaatcgc tcatagcctt aatcgttctc ctctcgcgat gcaatcagcc
     1441 atcatcagtt atacgctgac ggtggcgatg cttattccgg taagcggatg gctagccgat
     1501 cgcttcggta cgcgtcgcat ttttaccctt gccgtgagtc tgttcacgtt gggttctctg
     1561 gcctgcgcac tttccaactc gctaccacag ctggttgtct tccgggttat tcaggggata
     1621 ggcggcgcaa tgatgatgcc tgttgctcgg ctggccttac tgcgcgctta tcctcgtaat
     1681 gaacttcttc ctgtattgaa ttttgtcgcc atgccgggtc tggtggggcc aattttaggc
     1741 cccgttcttg gcggcgtgct ggtcacctgg gcaacctggc actggatatt tttaatcaat
     1801 atccccatag gtattgcggg ccttctttac gcgcgcaaac atatgcccaa tttcaccacc
     1861 gcacgacgca gattcgatac cactggcttt ttgctgtttg gcctcagcct tgttctcttc
     1921 tcaagcggaa tagagctatt cggggaaaag attgtcgcca gctggattgc cttgacggta
     1981 attgtcacca gcatctggtt actgcttctc tatattctcc atgcgcgacg cacgccaaac
     2041 ccattaattt cattagattt atttaaaacc cgcactttct cgatcggtat cgtaggcaat
     2101 attgcaaccc gtctggggac cggctgtgta ccgttcctta tgccattgat gttacaggta
     2161 ggatttgggt atcaggcgtt tattgctggc tgtatgatgg caccgacagc gttaggttcc
     2221 attattgcaa aatcgatggt tacccaagtc ttacgtcgtc tgggctatcg ccatacgtta
     2281 gtggggatca cggtgattat tgggctaatg atcgctcagt tctctttgca atcaccagca
     2341 atggcgatat ggatgctgat cttgccgttg tttatattag ggatggctat gtcgacgcaa
     2401 tttaccgcga tgaataccat cacacttgcc gatctgaccg atgacaacgc cagcagcggt
     2461 aacagtgttc tggcggtcac gcagcaactg tctatcagtt taggcgttgc tgtaagtgcg
     2521 gccgtccttc gcgtttacga agggatggaa ggcacaacga cagtcgaaca attccactat
     2581 acgtttatca cgatgggcat tattactgtt gcttcagcag caatgttcat gctactgaaa
     2641 acaaccgatg gtaataattt gatcaaaaga cagcgtaaat ctaagccgaa ccacgttcca
     2701 tcagaatcgg agtaagttgt aatcgttgtt gctgtagggt cggctgggtt atccgatgga
     2761 tgagtacatc aatcgccagc tcccccagtt catctttcgg ttggtgtata gttgttaatg
     2821 gtggcgtcat aaagcttgcc agttcgatat cgtcatagcc aatcaccgcg atatcctgcg
     2881 gaacctgtaa ctctgcctga tataacgcct ggtaaacgcc aacagccata gcgtcatttc
     2941 cggtaaagac ggcctgagga cgcagcggat gtgataacag ttggcgcata gcgtcaaacc
     3001 cgccgttaaa ttcaaaatca ccagtgactt catagccatc aggaatgttg agacccgcac
     3061 gtttcatcgc cgcccgataa ccttccaacc gcaggcgcgc cggagtttta tccagcggac
     3121 cggtaataca ggcaatacgg gtatgacctt tatcgatcag atattgcgtt gctaagtctc
     3181 cgcccagcaa cgagttatcc tgaataagat cgctgtcgcc atcgaacgga gcccagtcca
     3241 tcatcacggt aggcactgtc ggataacgtt gcatgatttc acgcgaaggc tgatgcgttt
     3301 cggtgcacag taacagcaag ccatcaacgc gtttttgcat cagcgtttcc agattgcgat
     3361 tcatccgctg ttcatcgcct tcggtattgc aaaggacaag actataaccg cgttcgaagc
     3421 agctgcgttc aacgccacgc accagttctg aatagaaagg attggtactg gcagtgatca
     3481 acatgccaat ggtatgtgtt tgattgagtt tgaggctacg cgccagagct gatggcgcgt
     3541 aattgagttc tttaatcgcc gcttcaactt tggctgtaat cgcttcactg acgaagcgat
     3601 ctttattgat aacgtgagaa actgttgagg tagaaacgcc cgccaggcgg gcaacatctt
     3661 tcattgtagc caagcgtcac ctctgcttgt ctaaaaatgc gtcgatctct tcacgccacg
     3721 gtacggaagg ttgtgcgcct ttacgtgtta cggcaatcgc agcggctgca tgggcaaaac
     3781 gaatcgcctc tggcaatggt ttttcttcca gcaacgccgt gattaacgcg ccgttaaagg
     3841 tatctccggc agcaatggta tcgacagccg gcacccggaa tccaggaacg cgctgacctt
     3901 caccattcac gctagcccat acaccacgac ttcctaaagt aatcagtaca gtacggatac
     3961 ctttttcatg cagtacctgc gccgccttcg ctgcatcttc atcattttca acacgaatac
     4021 cggtgagctt ttctgcttcc gtttcgtttg gcgtaataat gtccaccagc gccagcagtt
     4081 cgtcaggaag ttcgcgagcc ggagccgggt taagcgcaac gatagtctta ttttgatggg
     4141 cgattttcgc cgctgccatc acactttcga gtggtgattc cagctgcatt aataatgctg
     4201 acgcgttggc aatacgctca cgttgcgctt ccaccagcgc cggggaaagg gcagcattag
     4261 cgccggcatg aataccgatg acattctcac cttcgccatt aacaaaaatc agcgccacac
     4321 ctgttgattc gcctttgatc acgctgaccg gagaaatatc gatgttatcg gtggagagct
     4381 gctggcgaac gctctcgcca atgctgtcat cgccagtgca ggcaataaac gcgatatttg
     4441 caccgctacg tccagcagcc accgcctgat tcgcgccttt gccgccaaat gcaacctgat
     4501 agtggttacc ggttacggtt tcgcctggag taggaaaaga ttgaagatta agaatgtggt
     4561 cagcattaat gctgccaaga acaacgaggc tgcctgcgtt ttgcatattc gggatgtcca
     4621 taaaatgcgc caccgtatta gggtggcgcg tgccctgctt ttctttatat tacacgttca
     4681 tctgtcgatg acgtatttat gtcaccatca ggtcatacaa cctgattaaa actactgctt
     4741 aacaaccagt ttcagatcaa ccggatactt agcctgaact ttctctcctt tcagcacttt
     4801 atctgcggtt tcgacgcctt tcgcgccaat ctgatcgggt agctgagcga tagtcgctgc
     4861 tagtttgcca tcattcaccg ctttttcgcc atccggtgta ccgtcaaatc cgacgaccat
     4921 cacatccgat ttaccggcag tttgcagtgc gcgcagcgcg ccaagcgcca tttcatcatt
     4981 ctgcgcgaat acagcctgaa catccggatg agcggtcaac aggttctgca ttacgttcaa
     5041 gcctttggtg cgatcaaaat ctgcaggctg gctggcaaga acattaaact tgtgagcagc
     5101 aacggcctgc tggaaacctt cgccacgttc acgggctgcg gatgtaccgg caatgccttg
     5161 cagctcgata actttggcac cttcacccgc tttcttcgcg atgtaatcac cggcgatttt
     5221 gccgcccagt acgttatcag atgcaatgtg gctcaccact tcacctttcg ttgcctggcg
     5281 gtcaagagtg ataaccggga tgttcgcctg gttagccatc ttcacagcat tacctactgc
     5341 gtcggagtcg gtcgggttaa tcagcagaat tttggtgccg cggacggtta agtcctgcac
     5401 gttcgccagc tctttcgccg ggttgttctg ggagtccagc accaccaggt tatagccaag
     5461 tttatccgcc tctttctgcg cgccatcttt cagcgataca aagaacgggt tgttaagcgt
     5521 agagaccacc agcgcgatgg tgtcttttgc catcgcattc gcactgacgg tggcgcttag
     5581 cgcaacagcg gaaaccaggg tagccagttt tttcatgttc atattcaaga tgtcctgtag
     5641 tcgttattac tgctttttgt tgtctaccag caccgccagc aaaatcacca ccgctttgac
     5701 gatcatctgg taataggagg aaacacctaa caaattcaat ccattattaa ggaagccaag
     5761 aattaatgcg ccgatcaacg tcccaacaat gcgacctttt ccgcccgcca gactcgtacc
     5821 gcccagaacc accgccgcaa tagcatccag ctcatagcca gtccccgccg tgggttgtgc
     5881 ggaggagaga cgcgccactt caatgatccc ggccagcgat gccagcagac cacaaagaga
     5941 atagacgatg attttgattt tattgacgtt gataccagaa agacgcgttg ccgcttcgtt
     6001 gccgcccagc gcgtagatgt aacgccccag acgcgtgtga tgcagcatgt accaggccgc
     6061 gaggaagaca atccccatga tccagactgg cgtcggtacg cccagcggac gaccaatacc
     6121 aaaccagcca aacagatcgg cgttctcagt aaagccggta ttcactgggc taccattggt
     6181 ataaaccatg gtcacgccgc gcagtaaaag catcataacc agcgtagcga taaacgcctg
     6241 gacgcgacct ttcgctacaa tcaccccagt taccgcacca attgcggcac ctaacgcgag
     6301 agcggcagcg acagccacca gcgcattgac ttcaatgccg acgatagatg cagcaactgc
     6361 gccggtcagc gccaacagag aacctaccga caagtcgata cccgacgtca ggatcaccag
     6421 cgtcatcccg accgccataa tggcgttcac cgaggtttgc tgaagaatat tgaataagtt
     6481 attgatggtg aaaaagttcg ggcttaacgt cgagacaatc gcgatcagca ccagcagagc
     6541 gataagcgat ttctgctcca tcagccacgc tttcgtgaaa taacggcgac cagagacagt
     6601 ctgggttgtc atttttttac tcctgattca cgcgattaag cttgcccaca gcggcagcca
     6661 ttaacacttc ctgggtggcc tgctcacgag taaattcccc gctgagatgc ccttcatgca
     6721 tgacgatgat gcgatcgctc atgcctaata cttctggcat ctccgatgac accagaatga
     6781 tgctcaagcc atcggctttg aactggttaa tcagttgata gatctctttt ttcgcgccga
     6841 catctacgcc acgggtaggc tcatcgagga tcaacacttt agggcgtgtc atcagaccac
     6901 gggcaatcgc tactttttgc tgattgccac cggaaagcag accaattgcc tgttccatcg
     6961 atggcgtttt cacattaaac agacgaatga aatcactcac agcctgctgt tcatcggcat
     7021 gcttcaaact gccgccagcg cggctgaagt agcgcagcgc ggtcagcgac atgttctctt
     7081 ttactgacat gcccagcact aaaccgtcac gtttacggtc ttcggagata tacacaatgc
     7141 cgtttgccag gccatcctgc ggcgaacggg taacgacttc atgcccatcc agggtgacgt
     7201 aaccgcttgt gcgcggcagt gcgccgtaga gcactttcat cagttcggta cgcccagcgc
     7261 ccatcaaacc agagacgcca agaatttcgc ctttgcgtaa agtaaaagag acatcgttaa
     7321 cgccaggtcc gcagagatta tcgactttca ggcggatatc tcccggcgct ttgtccaggt
     7381 gcggatattg atcttccagc ttgcggccca ccatcatctc aatcagagaa tcttcggtca
     7441 gtgatgccac ttcacgctca gcaataaatt gtccgtcacg aaaaacggta acgtcatcgc
     7501 aaatctcgaa gatttctttc atgcggtggg agatatagac aataccgcgg ccttgcgatt
     7561 tcagctcgcg gatgacgcgg aacaaggatt cggtttcggt atcggtcagc gcatcggtcg
     7621 gttcatccat aatgatgact ttcgactcaa agctcagcac tttggcgatt tcaaccattt
     7681 gctggtcacc gatggaaaga tcacccacca gtttgtcgct tttaaagcgc aggttaagtt
     7741 tagccagcaa tttatccgct tcggcataca tggttttcca gtcaattttg ccaaagcgat
     7801 taacaaactc acgaccgagg aaaatgtttt cggcaatggt taactgcggg atcaggttca
     7861 gttcctggtg gataatccca atcccggctt cctgggaaga tttcggcccg gtaaatgtcg
     7921 tttctttccc caaccataaa agcgtaccgg catcgcgagt atagatacca gtaagcactt
     7981 tcatcatggt ggatttaccc gcgccgtttt cgcccaccag cgccatcacg cggcccggat
     8041 agacatttaa cgctgcgccc gagagggctt ttacgcccgg aaaggcttta tcgatgcctt
     8101 taagctgaag taatgcttcc atgacggcct cagaacgtca cgccagcaca gagaatgata
     8161 ttcgcatacg gagaacattc tccgctgcga attaccgcct gactttctgc ggtttgttgt
     8221 ttgaattgtt catgcgtggt gtaacgaatt tcaatggtgt ttccctggtg ttgttgcagc
     8281 tgctcaaggt gagtgagcaa cgtttcgtgg agttgcggat tatgttgttt gatctcttcc
     8341 gcgataatga cagcctcgac ctgcatttca tttgtgacga cgcccagcac ctgcataaaa
     8401 gaaggtacac cctgggttaa tgccatatcg atacgcgttg tacttttggg gatgggtaaa
     8461 ccagcatcac acaccaccag cgtatcggta tgtcccagtc gggagatcac cgatgaaata
     8521 tcagaattaa gaacggtgcc ttttttcatt tttttctcca tcagcgaaac gtttcgctga
     8581 cccgcagttt aatcctgact gagttgggaa aaccatcgtg acgttacgga aatgtgatca
     8641 acctcgaaac gttttacatg gtgattaacc atgaaaacaa aaacgccccc ttatggagaa
     8701 aggaggcgtc tggcgttaga tttcgacctg agtacccagt tcgataactc tgtttggcgg
     8761 gatttcaaat tgatccggtg cccgcagcgc attacgttgc agcagcaagt acagcttgcc
     8821 gcgcagacgc aaataccacg ggcgtttgcc gaggatcaac gactcatgcg acataaagaa
     8881 ggaggtttcc atcatccggc agcttaatcc ttccagaccg cagcggtgga aaacttcttc
     8941 cacgtttggc gtttctcgcc aaccataact tgccaccacg cgccagaaag tgggcgacag
     9001 ttgttcaatc tgtacccgac ggacgttatg gacatatggc gcgtcttcgg tgcgcagagt
     9061 taacagaatc acccgctcat gcaatacctt gttatgttta aggttatgca tcagcgcaaa
     9121 gggaatgacg ttgattgcac gcgacatata caccgcggtc ccaggcacgc gaacgggcgg
     9181 tgatttctcc agcgaagcaa tcatcgcttc cagagagtta ccatgttcat gcatccgccg
     9241 cagcaagcgg aaacgctcgc tcttccaggt ggtcatcacg ataaacatca ccgtaccgag
     9301 gctcaatggc aaccagccgc cggagagcag tttatcgagg ttagcggtga acaagggaat
     9361 atcgacacaa aggaaagcaa tcaggatcag cgcaacaaaa tacttattcc agtgccagtt
     9421 ctgacgtgcc acggtagtcg agagaataga cgtcagcacc atggttccgg tcaccgcaat
     9481 cccgtacgcc gccgccaggt tgctggagtg ctcaaagctg acaatcacaa tcacgaccgc
     9541 gacatagagc atccagttca caaagggaat atagatttgc cctgactcca tttcggaggt
     9601 gtgaataatg cgcatcggcg acaaatatcc cagacgtacc gcctgacgcg tcaatgagaa
     9661 gacgccagag ataaccgcct gcgaggcaat taccgtcgcc agtgcggcga tgatcagcag
     9721 cgggatcagc gcccagtccg gtgccaacag gaagaacggg ttcttaatcg cttccggatt
     9781 ctttaacaac agcgctccct ggccgaagta attaagggtt aaggaaggca atacgacggt
     9841 gaaccacgcc agacgaatag ggaacttacc aaagtgcccc atatcagcat acagcgcctc
     9901 gacccccgta atcgacagca ccactgcccc taatgcaata aaagaaaccg ttttgtattc
     9961 aaggaagaaa tgcaccgccc acatcggatt cagtgcatgc agcacttccg ggttagcaat
    10021 aatgctacgt aaccccagcc ctgccagaat caaaaaccag gtcagcatga tcggcgcaaa
    10081 cagcttaccg accatagctg tgccatgttt ttgaatcatg aataataatg tgagaacgat
    10141 aattgagagg ggaactatcc aggtatccag ctgcggggcg acgatttcca gaccttcaat
    10201 ggcggacatc accgaaatag cgggagtaat gacgacttca ccatagaaaa agctgccgcc
    10261 gattagcccc ataatcacca gcattgatgt ggttcgcgcc gacgtattac gcccggcaag
    10321 cgacatcaac gtcaggatcc ccccttcacc ggcgttatct gcccgcatca cgaaggtgag
    10381 atatttaatg gaaaccacaa agattagcag ccagaagatc agcgataaaa agccaaacac
    10441 ggcatcgcgt tcaacgccaa aaccaaactg gccggacaaa cattcacgaa gtgtatataa
    10501 cgggctggta ccaatatcgc cgtagacaac tccaatcgcc gcgagggtaa tcgcgggcaa
    10561 tgattgctta ttatcagtgc tcatagacta gtctttcgtt gaaatatgaa atgtgtgctt
    10621 agtcccttgg cccacaaaaa agcgcacagt atgcacgatt aacggcaaaa tcgtactcct
    10681 aaatgcgacc acattaacct ggctcaaaga aaaatacccc tcctttgaga ctattttagc
    10741 caccagcaag aaacgtctat actcgcaatt tacgcagaac ttttgatgaa aggacgccac
    10801 ttcattatgg ctcaccctca tttattagcg gaaagaattt cccgcctgag cagttcgttg
    10861 gaaaaggggc tttatgaacg tagccacgcc atccgcttgt gtttattagc ggcattaagt
    10921 ggtgaaagtg tgttcctcct tggcccgcca ggtattgcca aaagtttgat cgcccggcgc
    10981 ttaaaattcg cctttcagaa tgcccgcgcg tttgaatatc tgatgacccg cttctccacg
    11041 ccggaagaag tttttggtcc cctttctatt caggcgctaa aagatgaagg gcgctatgaa
    11101 cgtttaacca gcggttacct gccggaagct gaaatcgtct ttctggatga gatctggaaa
    11161 gcgggtccgg caattcttaa taccttgctc accgccatta acgagcgcca gttccgcaac
    11221 ggcgcactcg tagaaaaaat cccgatgcgt ctgctggtgg cggcctccaa cgagctgccg
    11281 gaagcagaca gcagtctgga agcgttatat gaccgcatgc tgattcgtct gtggttagat
    11341 aaagtgcagg ataaagcgaa tttccgctcc atgctgacca gtcaacagga tgaaaatgac
    11401 aatccggttc ctgcctcctt gcagatcaca gatgaagaat atgaacgctg gcagaaagag
    11461 attggtgaaa ttacgctgcc cgatcatgta tttgagctga tttttatgct gcgccagcaa
    11521 ctggataaat taccggatgc gccttatgtc tcggatcgtc gctggaaaaa agcgatccgc
    11581 ttattgcagg ccagcgcctt ttttagcggt cgcagtgctg ttgccccggt tgatctcatt
    11641 ttgctgaaag attgcctgtg gtatgacgcg caaagcctga atttgataca acaacaaatt
    11701 gatgtattga tgaccggtca cgcctggcaa cagcaaggga tgttgacccg cctgggcgcg
    11761 attgtgcaac gtcacctgca acttcagcag caacaaagcg ataaaacagc cttaacggta
    11821 attcgtctgg gcggcatttt tagccgtcgt cagcagtatc aactccctgt taacgttact
    11881 gcttccactc tgactctgct gctgcaaaaa ccgttaaaac tgcatgatat ggaagtggtt
    11941 catatctcct ttgagcgtag cgcgctggaa cagtggctga gtaaaggtgg tgagattcgc
    12001 ggcaaactga acggtatcgg ctttgcccag aaactgaatc tggaagtcga tagcgcccaa
    12061 catcttgttg tacgcgatgt gagtttacaa ggcagtacgc tagcacttcc cggttcatcg
    12121 gctgaaggtc tgcccagcga aataaaacaa caactggaag agcttgaaag cgactggcat
    12181 aagcaacacg ttttattcag cgaacagcaa aaatgtctgt ttatccctgg cgactggtta
    12241 ggtcgcattg aagccagcct acaggatgtc ggcgcacaga ttcgccaggc acaacaatgc
    12301 taacgctgga tacgcttaat gtgatgctgg ccgtcagcga agagggattg atcgaagaga
    12361 tgatcatcgc gctgctggcc tcaccgcagc tggcagtctt ctttgaaaaa ttccctcgac
    12421 tgaaggcggc aatcactgat gatgttcccc gctggcgtga ggcgctgcgc agtcggctga
    12481 aagatgcccg agtcccgccg gaactcaccg aagaggtgat gtgctatcag caaagccagc
    12541 tcctctccac gccgcagttt attgtgcagc taccacaaat cctggactta ctgcatcgtc
    12601 tgaattcccc atgggcagaa caagcccgac agttggttga tgctaacagt acgatcactt
    12661 cagcgttaca cacgcttttt ctccagcgct ggcgtttaag cctgatcgtg caagcaacga
    12721 cgttaaatca gcagctatta gaagaagaac gcgaacaact gttgagtgaa gttcaggaac
    12781 gcatgacgct gagcgggcaa cttgaaccga ttctcgcaga taacaatacc gcagctggtc
    12841 gtctgtggga tatgagcgcc ggtcagctta aacgtagcga ctatcagttg attgtgaaat
    12901 acggtgaatt tcttaacgaa cagccggaac tgaaacgtct ggcagaacag ctggggcgtt
    12961 cccgggaagc caaatcaata ccgcgcaacg atgcgcagat ggaaaccttc cgcaccatgg
    13021 tgcgcgaacc ggcgacagtt cctgagcagg tagatggtct gcagcaaagc gatgatattt
    13081 tacgtctctt gccgccagaa ctggcgacac tagggataac ggaactggag tatgagtttt
    13141 accgtcggct ggtggaaaaa cagttgctta cctatcgcct gcacggtgag tcgtggcgtg
    13201 aaaaaatgat cgaacgcccg gtggtgcata aagattacga cgaacagccg cgcggaccgt
    13261 ttattgtctg cgtggatact tccggctcaa tgggcggctt taatgaacag tgtgcgaaag
    13321 cgttctgcct ggccttgatg cgcatagccc tcgctgaaaa ccggcgctgc tatattatgc
    13381 tattttccac cgagatcgtc cgttatgagc tttcagggcc acaaggtatt gagcaagcga
    13441 tccgcttttt aagtcagcag tttcgtggcg gcaccgatct tgccagttgt tttcgcgcca
    13501 ttatggaacg cttgcaaagc agggaatggt ttgatgccga tgcggtggtg atttctgatt
    13561 ttatcgccca gcggttgcct gacgacgtga cgagtaaagt gaaagagttg cagcgggtac
    13621 atcagcatcg ctttcatgcc gtggcgatgt cggcacacgg caaacccggc atcatgcgca
    13681 ttttcgatca tatctggcgc tttgataccg ggatgcgaag ccgcctgctc agacgctggc
    13741 ggcgataaat tattacagca gagaagggac gctctcgcga acagctgccg gccatacacc
    13801 acactgaacc tggccgatat gcggcagttg cagcagcagc atggtcaaac gagactgccc
    13861 gataccgccg ccgatggtct gcggcatttc accgcgcagc agcgcctgat gccactccag
    13921 ctccaggcga tcttcgtcac cagtcagcgc cagctgatgc ttcagcgtgt cggcatccac
    13981 gcggatcccc atggaagaaa gctcaaacgc atcttccagt accgggttcc acaccagaat
    14041 atcgccgttc agacccgcat gacccagctc tgacggggtg ctccagtcat cataatccgg
    14101 tgcgcgcacg tcgtgacgat gaccatcact caacttgccg ccaatcccga cgaggaatac
    14161 cgcgccaaga tctttcgcaa tcgcccgctc acgccctttg gcatcgagat ccggataacg
    14221 agacagtaac tcctggctgt gtacgaagtg gatctgatcc ggcaggaacg gtgccaggcc
    14281 aaactcttcg ctaaccgcag cttcggttgc tttaattccc gcccagatcg cctctaccgt
    14341 gcttttcaga gtcgagaatt gacgctcgcc gtcgcccatt acgcgttccc agtcccactg
    14401 gtctacatag accgagtgca acggggaaag acggtcttca tcggggcgaa gggctttcat
    14461 gtgcgtgtac agcccttcgc ccgcgctgaa gtcgtgttgc cctaaggtct gacgtttcca
    14521 cttcgccagt gaatgaacca cttcgaactg ggcatcaggc agagctttca cttttacctg
    14581 caccgctttt tcacagcccg acaagttatc ctgcgtgcca tcccccacac ggctaagaat
    14641 cggcgcctgg acttcgatca gccccagacg ttcttccagt tgacgagaaa agtgagattt
    14701 cacgaagcta atttgacgtt gtttggcaat gtaagcggtt ttcatttttg atactcctgc
    14761 gtcctgttgc ttatgattaa gcaacaaaaa ccgacacaca tgcaataatc attcaataaa
    14821 aagcctcgcg tacttttgat tcattaaaaa agaaggctaa aatagaatga atcatcaatc
    14881 cacataagaa aatcctatgg aaaattatct gatcgacaat ctggaccgag gcatcctgga
    14941 agcattaatg ggcaatgcgc gcaccgctta cgccgaactg gcgaaacaat ttggcgtcag
    15001 tccggggacg attcacgttc gagtagagaa aatgaagcag gcggggatca ttaccggggc
    15061 gcgtattgat gtcagcccga agcagcttgg ttatgacgta ggctgcttta tcggcattat
    15121 attaaagagc gccaaagact atccttccgc gctggcaaag ctggaaagcc tcgatgaagt
    15181 cactgaagcc tattacacaa ccggccacta cagcatcttt ataaaagtga tgtgccgttc
    15241 gatcgatgct ctccagcatg tacttatcaa caagatccaa acaattgatg aaattcagtc
    15301 caccgagaca ttgatcgtcc tgcagaaccc gatcatgcgt accatcaagc cctgatcggc
    15361 ttttttaatc ccatactttt ccacaggtag atcccaacgc gttcacagcg tacaatacgc
    15421 cactcttaat aaaggtggcg gtttatggca gatatcactc ttatcagcgg cagcaccctc
    15481 ggcggtgccg aatatgtagc agaacacctg gctgaaaagc tggaagaggc gggttttacc
    15541 accgaaacgc tgcacggtcc gctgttggaa gatttatctg cctcggggat ctggctggtt
    15601 atcagctcaa cccacggtgc cggtgatatt ccggacaacc tttctccttt ctatgaagca
    15661 ttgcaggaac agaagcccga tctttctgcg gtccgctttg gcgcaatcgg tattggtagt
    15721 cgtgaatatg acaccttttg tggggctatc gataaactcg aggcagaact caaaaattcc
    15781 ggtgcaaaac agacaggcga aacactgaag atcaacattc ttgatcacga cattccggaa
    15841 gatccggcag aagaatggct gggatcgtgg attaatttac tcaaataagt atacagatcg
    15901 tgcgatctac tgtggataac tctgtcagga agcttggatc aaacggtagt tatccaaaga
    15961 acaactgttg ttcagttttt gagttgtgta taacccctca ttctgatccc agcttatacg
    16021 gtccaggatc accgatcatt cacagttaat gatcctttcc aggttgttga tcttaaaagc
    16081 cggatccttg ttatccacag ggcagtgcga tcctaataag agatcacaat agaacagatc
    16141 tctaaataaa tagatcttct ttttaatacc caggatccca ggtctttctc aagccgacaa
    16201 agttgagtag aatccacggc ccgggcttca atccattttc ataccgcgtt atgcgaggca
    16261 atcaccatgt tttatccgga tccttttgac gtcatcatca ttggcggggg tcatgcaggc
    16321 accgaggccg cgatggccgc ggcgcgtatg ggtcaacaga ctttgctttt gacacacaat
    16381 atcgacactc tggggcagat gagctgcaac ccggcgatcg gcggtattgg gaagggacat
    16441 ctggtaaaag aagtggatgc actcggcggt ctgatggcga aagcgatcga tcaggcgggt
    16501 atccagttta ggatactaaa cgcaagcaag ggaccggcgg ttcgcgctac ccgagctcag
    16561 gcagatcgtg tgctctaccg tcaggcggta cgtacggcgc tggagaacca accgaacctg
    16621 atgatcttcc agcaggcggt tgaagatctt attgtcgaaa acgatcgcgt ggtcggtgcc
    16681 gttacccaaa tgggactgaa gttccgtgcc aaagctgtcg tgctcaccgt tgggacgttc
    16741 ctcgacggta aaattcatat cggtctggat aattacagcg gtggccgtgc tggtgatccg
    16801 ccgtccattc cgctttctcg ccgtttgcgt gaactgccgc tgcgcgttgg tcgtctgaaa
    16861 accgggacac caccgcgtat tgatgctcga accatcgact ttagcgtact ggcgcaacag
    16921 catggcgata acccaatgcc ggtattctcg tttatgggca atgcgtccca gcatccccag
    16981 caggtgccgt gttatatcac tcataccaac gagaaaaccc atgatgtgat ccgcagtaac
    17041 ctcgatcgta gcccaatgta cgcaggggtg atcgaaggtg tcggcccacg ctactgcccg
    17101 tcgatcgaag acaaagtcat gcgctttgcc gacagaaatc agcatcagat cttccttgaa
    17161 ccggaagggc tgacctctaa cgaaatttat ccgaacggta tctccaccag cctgccgttc
    17221 gatgtgcaga tgcaaatcgt ccgctccatg caggggatgg aaaacgcgaa gatcgtgcgt
    17281 ccgggttatg ccattgagta tgacttcttc gatccacgcg acctgaaacc gacgctggag
    17341 agcaagttta tccaggggct gttctttgct ggtcagatta acggcactac cggttacgaa
    17401 gaagccgctg cgcaaggttt gctggctggt cttaacgctg cccgtctgtc tgctgacaaa
    17461 gaaggttggg ctccagcgcg ttctcaggca tatctcggcg tactggttga tgacctgtgc
    17521 actttaggaa ccaaagaacc gtatcgtatg tttacctcgc gcgcagaata tcgtctgatg
    17581 ctgcgcgaag ataatgcgga tctgcgtttg actgaaatcg gtcgtgaact gggcctggtg
    17641 gatgacgaac gttgggcgcg ctttaacgag aaacttgaga atatcgagcg tgagcgtcag
    17701 cgtctgaaat cgacctgggt aaccccgtcg gcggaagctg cagccgaagt gaatgctcac
    17761 ctgactgcgc cgctttcccg tgaagccagt ggtgaagatc tgctgcgtcg tcctgaaatg
    17821 acttatgaaa aattaaccac gctgacgccg tttgcccctg cgttgacaga cgaacaggcg
    17881 gcggaacagg ttgagattca ggttaaatac gaaggttata tcgcgcgcca gcaagatgag
    17941 atcgaaaagc agctgcgtaa cgagaatacc ctgctaccag cgacgctgga ttaccgccag
    18001 gtatccggtc tttctaacga agtgatcgcc aaacttaacg atcacaaacc ggcctctatt
    18061 ggccaggctt cgcgtatttc tggtgtcacg cctgcggcta tctccattct gctggtgtgg
    18121 ctgaaaaaac agggtatgct gcgtcgtagc gcataacgca ttaaaaatgc ctggtaagca
    18181 cccgcttacc aggcaacgca tcaagaacag gtaatcaccg tgctcaacaa actctcctta
    18241 ctgctgaaag acgcaggtat ttcgcttacc gatcaccaga aaaaccagct tattgcctac
    18301 gtgaatatgc tgcataaatg gaataaagcg tacaacctga cttcggttcg cgatcctaat
    18361 gagatgctgg tacgccatat tctcgatagc attgtggtgg caccgtatct gcaaggtgaa
    18421 cggtttatcg atgtcggcac cggaccggga ctgcctggca ttccactctc tatcgtgcgt
    18481 cctgaagccc atttcacgtt gttggatagc cttggtaaac gcgtgcgttt ccttcgtcag
    18541 gtgcaacatg agcttaaact ggagaatatt gagccagtac agagcagggt agaagagttt
    18601 ccgtcagagc cgccatttga tggcgtaatt agccgcgctt ttgcctctct gaacgatatg
    18661 gtgagctggt gccaccatct tcctggtgag caaggccgtt tctacgcgct gaaagggcaa
    18721 atgccggaag atgaaattgc tttgttgccc gaagaatatc aggtcgaatc agtggttaaa
    18781 cttcaggttc cagccctgga tggcgaacgt catctggtgg tgattaaagc aaataaaatt
    18841 taatttttat caaaaaaatc ataaaaaatt gaccggttag actgttaaca acaaccaggt
    18901 tttctactga tataactggt tacatttaac gccacgttca ctcttttgta tcaacaagat
    18961 aacgtggctt tttttggtaa gcagaaaata agtcataagt gaaaatatca gtctgctaaa
    19021 aatc
//
LOCUS       DAHHTG010000084        18919 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.084, whole genome shotgun sequence.
ACCESSION   DAHHTG010000084 DAHHTG010000000
VERSION     DAHHTG010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18919)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18919)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18919
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.084"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            310..1353
                     /gene="nadA"
                     /locus_tag="NBI74_003021"
     CDS             310..1353
                     /gene="nadA"
                     /locus_tag="NBI74_003021"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HCD1933313.1"
                     /translation="MSVMFDPDTAIYPFPPKPTPLSIDEKANYREKIKRLLKERNAVM
                     VAHYYTDPEIQQLAEETGGCISDSLEMARFGAKHPASTLLVAGVRFMGETAKILSPEK
                     TILMPTLQAECSLDLGCPVEEFNAFCDAHPDRTVVVYANTSAAVKARADWVVTSSIAV
                     ELIDHLDSLGEKIIWAPDKHLGRYVQKQTGADILCWQGACIVHDEFKTQALTRLQEEY
                     PDAAILVHPESPQAIVEMADAVGSTSQLIAAAKTLQHQRLIVATDRGIFYKMQQAVPD
                     KELLEAPTAGEGATCRSCAHCPWMAMNGLQAIAEALELEGSNHEVYVDERLRERALVP
                     LNRMLDFAATLRG"
     gene            1391..2110
                     /gene="pnuC"
                     /locus_tag="NBI74_003022"
     CDS             1391..2110
                     /gene="pnuC"
                     /locus_tag="NBI74_003022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HCD1933314.1"
                     /translation="MDFFSVQNILVHIPIGAGGYDLSWIEAVGTIAGLLCIGLASLEK
                     ISNYFFGLINVTLFGIIFFQIQLYASLLLQVFFFAANIYGWYAWSRQTSQNEAELKIR
                     WLPLPKALSWLAVCVVSIGLMTVFINPVFAFLTRVAVMIMQALGLQVAMPELQPDAFP
                     FWDSCMMVLSIVAMILMTRKYVENWLLWVIINVISVVIFALQGVYAMSLEYIILTFIA
                     LNGSRMWIKSARERGSRALSH"
     gene            complement(2107..3048)
                     /gene="zitB"
                     /locus_tag="NBI74_003023"
     CDS             complement(2107..3048)
                     /gene="zitB"
                     /locus_tag="NBI74_003023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family zinc transporter ZitB"
                     /protein_id="HCD1933315.1"
                     /translation="MAHSHSHTSSHLPEDNNARRLLYAFGVTAGFMLVEVIGGFLSGS
                     LALLADAGHMLTDTAALLFALLAVQFSRRPPTIRHTFGWLRLTTLAAFVNAIALVVIT
                     ILIVWEAIERFRTPRPVEGGMMMAIAVAGLLANILSFWLLHHGSEEKNLNVRAAALHV
                     LGDLLGSVGAIIAALIIIWTGWTPADPILSILVSLLVLRSAWRLLKDSVNELLEGAPV
                     SLDIAELKRRLCREIPEVRNVHHVHVWMVGEKPVMTLHVQVIPPHDHDALLDQIQHYL
                     MDHYQIEHATIQMEYQPCHGPDCHLNRGVSGHSHHHH"
     gene            complement(3162..3542)
                     /locus_tag="NBI74_003024"
     CDS             complement(3162..3542)
                     /locus_tag="NBI74_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgS-like family protein"
                     /protein_id="HCD1933316.1"
                     /translation="MKMTKLATLFLTATLSLASGAALAADSGAQTNNGQANAAADAGQ
                     VAPDARENVAPNNVDNNGVNTGSGGTMLHPDGSSMNNDGMTKDEEHKNTMCKDGRCPD
                     INKKVQTGDGINNDVDTKTDGTTQ"
     gene            3858..4910
                     /gene="aroG"
                     /locus_tag="NBI74_003025"
     CDS             3858..4910
                     /gene="aroG"
                     /locus_tag="NBI74_003025"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroG"
                     /protein_id="HCD1933317.1"
                     /translation="MNYQNDDLRIKEIKELLPPVALLEKFPATENAANTVAHARKAIH
                     KILKGNDDRLLVVIGPCSIHDPVAAKEYATRLLALREELKDELEIVMRVYFEKPRTTV
                     GWKGLINDPHMDNSFQINDGLRIARKLLLDINDSGLPAAGEFLDMITPQYLADLMSWG
                     AIGARTTESQVHRELASGLSCPVGFKNGTDGTIKVAIDAINAAGAPHCFLSVTKWGHS
                     AIVNTSGNGDCHIILRGGKEPNYSAKHVAEVKEGLNKAGLPAQVMIDFSHANSSKQFK
                     KQMDVCADVCQQIAGGEKAIIGVMVESHLVEGNQSLESGEPLAYGKSITDACIGWDDT
                     DALLRQLANAVKARRG"
     gene            complement(5077..5829)
                     /gene="gpmA"
                     /locus_tag="NBI74_003026"
     CDS             complement(5077..5829)
                     /gene="gpmA"
                     /locus_tag="NBI74_003026"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000301556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HCD1933318.1"
                     /translation="MAVTKLVLVRHGESQWNKENRFTGWYDVDLSEKGVSEAKAAGKL
                     LKEEGYSFDFAYTSVLKRAIHTLWNVLDELDQAWLPVEKSWKLNERHYGALQGLNKAE
                     TAEKYGDEQVKQWRRGFAVTPPELTKDDERYPGHDPRYAKLSEKELPLTESLALTIDR
                     VIPYWNETILPRMKSGERVIIAAHGNSLRALVKYLDNMSEEEILELNIPTGVPLVYEF
                     DENFKPLKRYYLGNADEIAAKAAAVANQGKAK"
     gene            complement(6032..7072)
                     /gene="galM"
                     /locus_tag="NBI74_003027"
     CDS             complement(6032..7072)
                     /gene="galM"
                     /locus_tag="NBI74_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-epimerase"
                     /protein_id="HCD1933319.1"
                     /translation="MLNETPALAPDGQPYRLLTLRNNAGMVVTLMDWGATLLSARIPL
                     SDGSVREALLGCASPECYQDQAAFLGASIGRYANRIANSRYTFDGETVTLSPSQGVNQ
                     LHGGPEGFDKRRWQIVNQNDRQVLFALSSDDGDQGFPGNLGATVQYRLTDDNRISITY
                     RATVDKPCPVNMTNHVYFNLDGEQSDVRNHKLQILAEEYLPVDEGGIPHDGLKSVAGT
                     SFDFRNAKIIASEFLADDDQRKVKGYDHAFLLQAKGDGKKVAAHVWSADEKLQLKVYT
                     TAPALQFYSGNFLGGTPSRGTEPYADWQGLALESEFLPDSPNHPEWPQPDCFLRPGEE
                     YSSLTEYQFIAQ"
     gene            complement(7066..8214)
                     /gene="galK"
                     /locus_tag="NBI74_003028"
     CDS             complement(7066..8214)
                     /gene="galK"
                     /locus_tag="NBI74_003028"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001713229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HCD1933320.1"
                     /translation="MSLKEKTQSLFANAFGYPATHTIQAPGRVNLIGEHTDYNDGFVL
                     PCAIDYQTVISCAPRDDRKVRVMAADYENQLDEFSLDAPIVAHENYQWANYVRGVVKH
                     LQLRNNSFGGVDMVISGNVPQGAGLSSSASLEVAVGTVLQQLYHLPLDGAQIALNGQE
                     AENQFVGCNCGIMDQLISALGKKDHALLIDCRSLGTKAVSMPKGVAVVIINSNFKRTL
                     VGSEYNTRREQCETGARFFQQPALRDVTIEEFNAVAHELDPIVAKRVRHILTENARTV
                     EAASALEQGDLKRMGELMAESHASMRDDFEITVPQIDTLVEIVKAVIGDKGGVRMTGG
                     GFGGCIVALIPEELVPAVQQAVAEQYEAKTGIKETFYVCKPSQGAGQC"
     gene            complement(8218..9264)
                     /gene="galT"
                     /locus_tag="NBI74_003029"
     CDS             complement(8218..9264)
                     /gene="galT"
                     /locus_tag="NBI74_003029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HCD1933321.1"
                     /translation="MTQFNPVDHPHRRYNPLTGQWILVSPHRAKRPWQGAQETPAKQV
                     LPAHDPDCFLCAGNVRVTGDKNPDYTGTYVFTNDFAALMSDTPDAPESNDPLMRCQSA
                     RGTSRVICFSPDHSKTLPELSVAALTEIVKTWQEQTAELGKTYPWVQVFENKGAAMGC
                     SNPHPHGQIWANSFLPNEAEREDRLQKEYFAGQKSPMLVDYVQRELADGSRTVVETEH
                     WLAVVPYWAAWPFETLLLPKAHVLRITDLTDAQRSDLALALKKLTSRYDNLFQCSFPY
                     SMGWHGAPFNGEENQHWQLHAHFYPPLLRSATVRKFMVGYEMLAETQRDLTAEQAAER
                     LRAVSDIHFRESGV"
     gene            complement(9274..10290)
                     /gene="galE"
                     /locus_tag="NBI74_003030"
     CDS             complement(9274..10290)
                     /gene="galE"
                     /locus_tag="NBI74_003030"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308814.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HCD1933322.1"
                     /translation="MRVLVTGGSGYIGSHTCVQLLQNGHDVIILDNLCNSKRSVLPVI
                     ERLGGKHPTFVEGDIRNEALMTEILHDHAIDTVIHFAGLKAVGESVQKPLEYYDNNVN
                     GTLRLISAMRAANVKNFIFSSSATVYGDQPKIPYVESFPTGTPQSPYGKSKLMVEQIL
                     TDLQKAQPDWSIALLRYFNPVGAHPSGDMGEDPQGIPNNLMPYIAQVAVGRRDSLAIF
                     GNDYPTEDGTGVRDYIHVMDLADGHVVAMEKLANKPGVHIYNLGAGVGSSVLDVVNAF
                     SKACGKPVNYHFAPRRAGDLPAYWADASKADRELNWRVTRTLDEMAQDTWHWQSRHPQ
                     GYPD"
     gene            complement(10553..12025)
                     /gene="modF"
                     /locus_tag="NBI74_003031"
     CDS             complement(10553..12025)
                     /gene="modF"
                     /locus_tag="NBI74_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter ATP-binding protein
                     ModF"
                     /protein_id="HCD1933323.1"
                     /translation="MSSLQILQGTFRLGDTKTLQLPRLTLNAGDSWAFVGSNGSGKSA
                     LARALSGELPLLKGERQSQFSHITRLSFEQLQKLVSDEWQRNNTDMLSPGEDDTGRTT
                     AEIIQDEVKDTSRCMQLAQQFGITALLDRRFKYLSTGETRKTLLCQALMSEPDLLILD
                     EPFDGLDVASRQHLAELLASLHQSGITLVLVLNRFDEIPEFVQFAGVLADCTLAETGA
                     KEELLQQALVAQLAHSELLEGVQMPEPDEPSARHALPANEPRIVLNNGVVSYNERPIL
                     NNLSWQVNPGEHWQIVGPNGAGKSTLLSLITGDHPQGYSNDLTLFGRRRGSGETIWDI
                     KKHIGYVSSSLHLDYRVSTTVRNVILSGYFDSIGIYQAVSDRQQKLVQQWLDILGIDK
                     RTADAPFHSLSWGQQRLALIVRALVKHPTLLILDEPLQGLDPLNRQLIRRFVDVLISE
                     GETQLLFVSHHAEDAPACITHRLEFVPDGGLYRYVLTKIN"
     gene            complement(12093..12881)
                     /gene="modE"
                     /locus_tag="NBI74_003032"
     CDS             complement(12093..12881)
                     /gene="modE"
                     /locus_tag="NBI74_003032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum-dependent transcriptional regulator"
                     /protein_id="HCD1933324.1"
                     /translation="MQAEILLTLKLQQKLFADPRRISLLKHIALSGSISQGAKDAGIS
                     YKSAWDAINEMNQLSEHILVERATGGKGGGGAVLTRYGQRLIQLYDLLAQIQQKAFDV
                     LSDDDALPLNSLLAAISRFSLQTSARNQWFGTITARDHDDVQQHVDVLLADGKTRLKV
                     AITAQSGARLGLDEGKEVLILLKAPWVGITQDEAVAQNADNQLPGIISHIERGAEQCE
                     VLMALPDGQTLCATVPVNEATSLQQGQNVTAYFNADSVIIATLC"
     gene            13010..13159
                     /gene="acrZ"
                     /locus_tag="NBI74_003033"
     CDS             13010..13159
                     /gene="acrZ"
                     /locus_tag="NBI74_003033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump accessory protein AcrZ"
                     /protein_id="HCD1933325.1"
                     /translation="MLELLKSLVFAVIMVPVVMAIILGLIYGLGEVFNIFSGVGKKDQ
                     PGQNH"
     gene            13326..14099
                     /gene="modA"
                     /locus_tag="NBI74_003034"
     CDS             13326..14099
                     /gene="modA"
                     /locus_tag="NBI74_003034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HCD1933326.1"
                     /translation="MARKWLNLFTGAALSFAVAGNALADEGKITVFAAASLTNAMQDI
                     ATQYKKEKGVDVVSSFASSSTLARQIEAGAPADLFISADQKWMDYAVDKKAIDTASRQ
                     TLLGNSLVVVAPKASEQKDFTIDSKTNWTSLLNGGRLAVGDPEHVPAGIYAKEALQKL
                     GAWDTLSPKLAPAEDVRGALALVERNEAPLGIVYGSDAVASKGVKVVATFPEDSHKKV
                     EYPVAVVEGHNNATVKAFYDYLKGPQAAEIFKRYGFTTK"
     gene            14099..14788
                     /gene="modB"
                     /locus_tag="NBI74_003035"
     CDS             14099..14788
                     /gene="modB"
                     /locus_tag="NBI74_003035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HCD1933327.1"
                     /translation="MILTDPEWQAVLLSLKVSSLAVLFSLPFGIFFAWLLVRCTFPGK
                     ALLDSVLHLPLVLPPVVVGYLLLVSMGRRGFIGERLYDWFGLSFAFSWRGAVLAAAVM
                     SFPLMVRAIRLALEGVDIKLEQAARTLGAGRWRVFFTITLPLTLPGIIVGTVLAFARS
                     LGEFGATITFVSNIPGETRTIPSAMYTLIQTPGGESGAARLCIISIALAMISLLISEW
                     LARISRERAGR"
     gene            14791..15849
                     /gene="modC"
                     /locus_tag="NBI74_003036"
     CDS             14791..15849
                     /gene="modC"
                     /locus_tag="NBI74_003036"
                     /EC_number="7.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum ABC transporter ATP-binding protein
                     ModC"
                     /protein_id="HCD1933328.1"
                     /translation="MLELNFSQTLGNHCLTINETLPANGITAIFGVSGAGKTSLINAI
                     SGLTRPQKGRIVLNGRVLNDADKGICLTPEKRRVGYVFQDARLFPHYKVRGNLRYGMA
                     KSMVNQFDKLVALLGIEPLLDRLPGSLSGGEKQRVAIGRALLTAPELLLLDEPLASLD
                     IPRKRELLPYLQRLTREINIPMLYVSHSLDEILHLADRVMVLENGQVKAFGALEEVWG
                     SSVMNPWLPKEQQSSILKVTVLEHHPHYAMTALALGDQHLWVNKLDEPLQAALRIRIQ
                     ASDVSLVLQPPQQTSIRNVLRAKVVNSYDDNGQVEVELEVGCKTLWARISPWARDELA
                     IKPGLWLYAQIKSVSITA"
     gene            complement(15850..16668)
                     /gene="ybhA"
                     /locus_tag="NBI74_003037"
     CDS             complement(15850..16668)
                     /gene="ybhA"
                     /locus_tag="NBI74_003037"
                     /EC_number="3.1.3.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyridoxal
                     phosphate/fructose-1,6-bisphosphate phosphatase"
                     /protein_id="HCD1933329.1"
                     /translation="MTTRVIALDLDGTLLTPKKTLLPSSIEALARAREAGYRLIIVTG
                     RHHVAIHPFYQALALDTPAICCNGTYLYDYHAKTVLEADPMPVNKALQLIEMLNEHHI
                     HGLMYVDDAMVYEHPTGHVIRTSNWAQTLPPEQRPTFTQVASLAETAQQVNAVWKFAL
                     THDDLPQLQHFGKHVEHELGLECEWSWHDQVDIARGGNSKGKRLTKWVEAQGWSMENV
                     VAFGDNFNDISMLEAAGTGVAMGNADDAVKARANIVIGDNTTDSIAQFIYSHLI"
     gene            16823..17818
                     /gene="pgl"
                     /locus_tag="NBI74_003038"
     CDS             16823..17818
                     /gene="pgl"
                     /locus_tag="NBI74_003038"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HCD1933330.1"
                     /translation="MKQTVYIASPESQQIHVWNLNHEGALTLTQVVDVPGQVQPMVVS
                     PDKRYLYVGVRPEFRVLAYRIAPDDGALTFAAESALPGSPTHISTDHQGQFVFVGSYN
                     AGNVSVTRLEDGLPVGVVDVVEGLDGCHSANISPDNRTLWVPALKQDRICLFTVSDDG
                     HLVAQDPAEVTTVEGAGPRHMVFHPNEQYAYCVNELNSSVDVWELKDPHGNIECVQTL
                     DMMPENFSDTRWAADIHITPDGRHLYACDRTASLITVFSVSEDGSVLSKEGFQPTETQ
                     PRGFNVDHSGKYLIAAGQKSHHISVYEIVGEQGLLHEKGRYAVGQGPMWVVVNAH"
     gene            complement(17859..18812)
                     /locus_tag="NBI74_003039"
     CDS             complement(17859..18812)
                     /locus_tag="NBI74_003039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308823.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCD1933331.1"
                     /translation="MKHELSSMKAFVILAESSSFNNAAKLLNITQPALTRRIKKMEED
                     LHIQLFERTTRKVTLTKAGKRLLPEARELIKKFDETLFSIRDMNAYHRGMVTLACIPT
                     AVFYFLPLAIGKFNELYPNIKVRILEQGTNNCMESVLCNESDFGINMNNVTNSSIDFT
                     PLVNEPFVLACRRDHPLAKKQLVEWQELVGYKMIGVRSSSGNRLLIEQQLADKPWKLD
                     WFYEVRHLSTSLGLVEAGLGISALPGLAMPHAPYSSIIGIPLVEPVIRRTLGIIRRKD
                     AVLSPAAERFFALLINLWTDDKDNLWTNIVERQRHALQEIG"
BASE COUNT         4503 a         4703 c         4945 g         4768 t
ORIGIN      
        1 ggtcgcaggt tcgaatcctg cacgacccac caatgcaaaa aagcgcccta aaggcgtttt
       61 tttgctattc aggcatctct caatttcaat tcttgaccct gatgacatac tccgcgtttg
      121 ctgcgtaaac aactctacgt cggaggaata agacgcaaag tgtggcattc ggcatctcat
      181 acgtaattcg agtgactttt ccccaccatt cgactatctt gtttagtata taaaacaaat
      241 tacgccgata atagcgaata tcacgcttaa gtcggtttta acgttaagcc tgtagaacga
      301 gatggtaaga tgagcgtgat gtttgatcca gacacggcga tttatccttt ccccccgaag
      361 ccgacgccgt taagcattga tgaaaaagcg aattaccgcg agaagataaa acgtctacta
      421 aaagaacgta atgcggtgat ggttgcccac tactataccg atcccgaaat tcaacaactg
      481 gcagaagaaa ccggtggctg tatttctgat tctctggaaa tggcgcgctt cggtgcaaag
      541 catcccgctt ctacgttgct agtcgctggg gtgagattta tgggagaaac cgccaaaatt
      601 ctcagtccgg aaaaaacaat tctgatgccg acacttcagg ctgaatgttc actggatctc
      661 ggctgccctg ttgaagaatt taacgcattt tgcgatgccc atcccgatcg tactgtcgtc
      721 gtctacgcca acacttctgc tgcggtaaaa gcgcgcgcag attgggtggt aacttcaagc
      781 attgccgtcg aacttattga tcatctggat agtttgggtg aaaaaatcat ctgggcacct
      841 gacaaacatc tggggcgtta cgtgcaaaaa cagacgggcg cagacatttt atgctggcag
      901 ggtgcctgca ttgtgcatga tgagtttaag actcaggcgt taacccgctt gcaagaagaa
      961 tacccggatg ctgccatact ggtgcatcca gaatcaccgc aagctattgt agagatggcg
     1021 gatgcggtcg gttccaccag tcaactgatc gctgctgcga aaacattgca acatcagcgg
     1081 cttatcgtgg caaccgatcg gggcattttc tacaaaatgc agcaggcggt gccagataaa
     1141 gagttactgg aagcaccaac cgcgggtgag ggcgcaacct gtcgcagctg cgcgcattgt
     1201 ccgtggatgg ccatgaatgg ccttcaggcc atcgcagagg cattagaact ggaaggaagc
     1261 aatcacgagg tttatgttga tgaaaggctg cgagagaggg cgctggtgcc gctcaatcgt
     1321 atgctggatt ttgcggctac actacgtgga taattgataa taaggcgtca cattacgctt
     1381 tgggggaacg atggattttt ttagtgtgca gaatatcctg gtacatatac caataggggc
     1441 aggcggttat gatctctcat ggatcgaagc ggtaggcacg atcgccgggt tgctgtgtat
     1501 tggccttgcc agtctggaga agatcagtaa ctacttcttt ggcctgatca acgtcacctt
     1561 gtttggcatt attttctttc agattcagct gtatgccagc ctgttattac aggtgttttt
     1621 ctttgccgcg aatatttacg gctggtatgc gtggtcgcga caaaccagtc agaacgaggc
     1681 ggagttgaaa attcgctggt tgccattgcc gaaggcgcta agctggctgg cggtttgcgt
     1741 tgtttcgatt ggtctaatga cggtatttat caatcctgtg tttgcatttt tgacccgcgt
     1801 ggcagtcatg atcatgcaag cattaggatt acaggttgcg atgcctgaac tacagccgga
     1861 cgctttcccg ttctgggatt catgcatgat ggtgttatct atcgtggcaa tgattctgat
     1921 gacacgtaag tatgtggaaa actggctgtt gtgggtgatt attaacgtga ttagcgtcgt
     1981 tatttttgca cttcagggcg tttacgccat gtctctggag tacatcatcc tgacctttat
     2041 tgccctcaac ggcagccgga tgtggatcaa aagcgcacgc gaaagaggtt cacgtgcgct
     2101 gtcccattaa tgatgatgat gtgaatggcc agacacccct ctattcaggt ggcagtctgg
     2161 cccatgacaa ggttgatatt ccatctgaat ggtggcgtgc tcaatctgat agtgatccat
     2221 caggtagtgt tggatctgat ccaacaaggc atcgtgatcg tgtggcggga tcacctgcac
     2281 atgcagcgtc atcaccggct tctcgcctac catccataca tgcacatggt gcacattgcg
     2341 gacttccggg atttcccggc acaagcgacg cttcagttca gcgatatcca gcgataccgg
     2401 tgcaccttca agtaattcat tcacactatc tttcaacagt cgccacgcgc tacgcagaac
     2461 cagaagcgac accagtattg agagaatggg gtcagcaggt gtccagccgg tccagataat
     2521 aatcaacgcg gcaataatag ccccaaccga acccagcagg tccccgagca catgcagtgc
     2581 cgctgctcgc acgttgaggt ttttctcttc gctgccgtga tgaagtaacc agaaagaaag
     2641 tatatttgcc agcagcccag ccacggcaat tgccatcatc atgccgccct cgaccggacg
     2701 cggagtgcgg aagcgttcta tcgcctccca gacaatcaaa atagtaatca ccaccagggc
     2761 gatggcattc acaaacgccg cgagcgtggt caatcttagc caaccgaaag tgtggcgaat
     2821 ggtgggagga cgacgggaaa attgcactgc gagtagggcg aaaagcaggg cggcagtatc
     2881 ggtcaacata tgacccgcat cggccagcaa tgccagagaa ccggaaagaa aaccaccaat
     2941 gacttctacg agcataaacc cggcagtcac gccgaaagca tacaacaagc gacgagcatt
     3001 attatcttct ggcaggtgtg aggaagtgtg tgagtgtgag tgcgccatga cgagtcatcc
     3061 cttaattatt ctcacttaac tttatgacat catactgctt ttagaaggga aaaattaaaa
     3121 gggagagact acgctctccc attattggct atttcgcagg gttactgcgt ggtaccgtcg
     3181 gttttggtat cgacatcatt gttgatgcca tcaccggttt ggactttttt attgatatcc
     3241 gggcagcgac catctttgca catggtgttc ttgtgctctt catctttggt catgccgtca
     3301 ttgttcattg aagaaccatc cggatgcagc attgtgccgc cagaaccggt atttaccccg
     3361 ttattgtcga cgttatttgg cgcgacattt tcacgggcat caggggctac ctggcccgca
     3421 tcagcggcgg cgtttgcctg gccgttatta gtttgcgctc cgctatcggc agccagtgcg
     3481 gcaccgctgg caaggcttag agtggcagtc aaaaatagtg tggccagttt tgtcattttc
     3541 ataggatgct cctgttatgg tcgttatgtc ggataacctc ttccaacagt gcatttgcag
     3601 gtgaatataa ggcaatggtt taagatttca gccaggcaaa gatacccagc agagaatctt
     3661 gaaataatta acaaacaaag gagttacagt tagaaattgt aggagagatc tcgtttttcg
     3721 cgacaatctg gcgtttttct tgctaattcc aggattaatc cgttcatagt gtaaaacccc
     3781 gtttacacat tctgacggaa gatatagatt ggaagtattg cattcactaa gataagtatg
     3841 gcaacactgg aacagacatg aattatcaga acgacgattt acgcatcaaa gaaatcaaag
     3901 agttacttcc tcctgtcgca ttgctggaaa aattccccgc tactgaaaat gccgcgaata
     3961 cggtcgccca tgcccgaaaa gcgatccata agatcctgaa aggtaatgat gatcgcctgt
     4021 tggtggtgat tggcccatgc tcaattcatg atcctgtcgc ggctaaagag tatgccactc
     4081 gcttgctggc gctgcgtgaa gagctgaaag atgagctgga aatcgtaatg cgcgtctatt
     4141 ttgaaaagcc gcgtaccacg gtgggctgga aagggctgat taacgatccg catatggata
     4201 acagcttcca gatcaacgac ggtctgcgta ttgcccgtaa attgttgctt gatattaacg
     4261 acagcggtct gccagcggcg ggtgagtttc tcgatatgat caccccacaa tatctcgctg
     4321 acctgatgag ctggggcgca attggcgcac gtactacgga atcgcaggtg caccgcgaac
     4381 tggcttcagg gctttcttgc ccggtaggct tcaaaaatgg cactgatggt acgattaaag
     4441 tggctatcga tgccattaat gccgccggtg cgccgcactg cttcctttca gtgaccaaat
     4501 ggggtcattc ggcgattgtg aataccagcg gtaacggcga ttgccatatc attctgcgcg
     4561 gcggtaaaga gcctaactac agcgcgaagc acgttgctga agtgaaagaa gggctgaaca
     4621 aagcaggcct gccagcacag gtgatgatcg atttcagcca tgctaactcg tctaaacaat
     4681 tcaaaaagca gatggatgtt tgtgctgacg tttgccagca gattgccggt ggcgaaaagg
     4741 ccattattgg cgtgatggtg gaaagccatc tggtggaagg caatcagagc ctcgagagcg
     4801 gggaaccgct ggcctacggt aagagcatca ccgatgcctg cattggctgg gatgataccg
     4861 atgctctgtt acgtcaactg gcgaatgcag taaaagcgcg tcgcgggtaa agtttaattg
     4921 tcggatgcgc tgtcagagtg gcgtatccga ttaatcaccg taggcctgat aaaacgcgca
     4981 gcgtcgcatc aggcaatgcg ctccattggt agcaacgaaa aataaaaaag ccgactcatt
     5041 ttgcagtcgg ctttctcatt tttaagcgaa tgacgtttac ttcgctttac cctggttagc
     5101 aaccgccgct gctttcgccg cgatctcgtc agcattaccc agataatagc gtttcagcgg
     5161 tttgaagttc tcgtcaaact cataaaccag cggcacgccg gtcgggatat tcagctcaag
     5221 aatctcttct tcgctcatgt tatcgagata tttcaccagc gcacgtaacg agttaccgtg
     5281 tgcggcgatg atgacgcgct caccgctctt catacgcggc agaatggttt cattccagta
     5341 agggatcacg cggtcaatgg tcagcgccag gctttccgtc agtggcagct ctttctcgct
     5401 cagtttcgcg taacgcggat cgtgacccgg ataacgctca tcatctttag tcagttccgg
     5461 cggagtcacc gcaaaaccac gacgccactg tttcacctgc tcgtcgccat acttttcagc
     5521 ggtttccgct ttgttcagac cctgcaatgc accgtagtga cgttcgttca gtttccagga
     5581 tttctcaacg ggcagccatg cctgatccag ttcgtccagc acattccaca gggtatgaat
     5641 agcgcgtttc agcacagagg tgtaagcaaa gtcaaagctg taaccttcct ctttcagcag
     5701 cttacctgct gcttttgctt cgcttacgcc tttctcagac agatccacgt cgtaccaacc
     5761 ggtgaaacgg ttttctttgt tccactgact ttcgccatga cgaaccagaa ccagcttagt
     5821 tacagccata tacttactcc tcaaatcatc ttttaatgat aataattctc attatattgc
     5881 cgtgacgaag caacagcaat gcttacgcat aaccatagcg aaaatagtgt cgcagtgtaa
     5941 ggttgttgtg aatattgagt tgcaaatatg tcggtgtttg ctggtgattt gaacaatatg
     6001 aggataaagc cctcatgacg agggcttaac attactgagc aataaactga tattccgtca
     6061 ggctggaata ctcttcgcca ggacgcagga agcagtccgg ttgcggccat tcagggtggt
     6121 tcgggctgtc cggcagaaat tcgctttcca gagccagccc ttgccagtca gcgtaaggtt
     6181 cggttccccg cgacggtgtg ccgccgagga agttgccgga gtagaattgc agagccggag
     6241 cggtggtgta gaccttcagc tgcaattttt catctgctga ccagacatgc gccgccactt
     6301 tcttgccatc gcctttggcc tgtaacaaga acgcgtgatc gtaacctttc actttgcgct
     6361 gatcgtcgtc ggcaagaaac tcactggcga tgattttggc gttgcggaaa tcaaaagagg
     6421 ttccggcgac agatttcagg ccgtcgtgcg gaatgccgcc ttcatcaacc ggcagatatt
     6481 cttccgccag aatctgcaac ttatgattgc gcacgtcaga ctgctcgccg tcaagattga
     6541 aatagacgtg attagtcata ttcaccgggc aaggtttatc aactgtggcg cgataagtaa
     6601 tggagatacg gttatcgtcg gtcagacgat attgcaccgt cgcgccgaga ttacccggga
     6661 agccctgatc gccatcatct gaactcaggg caaacagcac ctgacgatcg ttctggttca
     6721 caatctgcca gcgacgtttg tcgaaccctt ccgggccgcc atgcaattgg ttgacgccct
     6781 gacttggtga aagcgtcacg gtttcaccgt caaaggtata acggctattg gcgatgcggt
     6841 tggcataacg accaatagag gcccccagaa atgcggcctg atcctgatag cattccgggc
     6901 tggcgcagcc aagcagcgcc tcgcggacgc tgccatcgga aagcggaata cgggcggaaa
     6961 gtaaagtcgc accccagtcc atcagcgtga ctaccatccc tgcgttgtta cgcaaagtta
     7021 acagtcggta cggctgacca tcgggtgcca gtgcgggagt ttcgttcagc actgtcctgc
     7081 tccttgtgat ggtttacaaa cgtaaaaagt ctctttaata cctgtttttg cttcatattg
     7141 ttcagcgaca gcttgctgta cggcaggcac cagttcttcc gggatcaacg cgacgataca
     7201 gccaccaaat ccgccgccgg tcatgcgtac gccacctttg tcgccaatca cagctttgac
     7261 gatttctacc agagtgtcaa tttgcggtac ggtgatttcg aaatcatcgc gcatagaggc
     7321 atgagactcc gccatcaact cgcccatacg tttcaggtcg ccttgctcca gcgcgctggc
     7381 agcttcaacg gtgcgggcgt tttcagtcag gatgtgacgc acgcgtttcg cgacgatcgg
     7441 gtccagttca tgtgcaacag cgttgaactc ttcaatggtg acatcgcgca gagctggctg
     7501 ctggaagaaa cgcgcaccgg tttcgcactg ttcacgacgg gtgttgtatt cgctgccaac
     7561 cagggtacgt ttgaagttac tgttgatgat gacgacagcc acgcctttcg gcatggaaac
     7621 agctttggtc cccagtgagc ggcaatcgat cagcaaggca tgatctttct tgccgagcgc
     7681 ggaaatcagt tgatccatga ttccacagtt acagcctaca aactggtttt ctgcttcctg
     7741 accgttaagc gcgatttgtg cgccgtccag cggcagatga taaagctgct gcaatacggt
     7801 tccgaccgcg acttccagtg aagcggaaga acttaaccct gcaccctgcg gcacattgcc
     7861 gctgatcacc atgtccacac cgccgaagct gttgttacgc agttgtagat gtttcaccac
     7921 gccacgaacg tagttcgccc attgatagtt ttcatgcgcg acaatgggcg catcgaggga
     7981 aaactcgtcg agctgatttt cataatcggc tgccattacg cgaactttac ggtcatcgcg
     8041 tggcgcacag ctgatcacgg tttgataatc aatcgcgcag ggcagaacga aaccgtcgtt
     8101 gtagtcggtg tgttcaccaa tcaaattcac gcggccaggc gcctgaatgg tgtgagtggc
     8161 agggtagcca aatgcgttgg caaacagaga ttgtgttttt tctttcagac tcatttctta
     8221 cactccggat tcgcgaaaat ggatatcgct gactgcgcgc aaacgctctg cggcctgttc
     8281 tgcggtcagg tctcgctggg tttctgccag catttcataa ccaaccataa atttacgtac
     8341 ggtggcagag cgcaacagag gcggataaaa gtgcgcgtgc agctgccagt gttgattctc
     8401 ttcgccatta aatggcgcgc cgtgccagcc catagagtag gggaaggagc actggaagag
     8461 gttgtcataa cgactggtca gctttttcaa cgccagagcc aaatcgctgc gctgggcgtc
     8521 ggtcaaatcg gtgatccgta aaacgtgggc tttgggcagc agtagcgttt cgaacggcca
     8581 ggcagcccag taaggcacga cggctaacca gtgttcggtt tcgacaacgg tacggctacc
     8641 gtctgccagc tcgcgctgaa cataatccac cagcattggt gatttctgcc cggcgaaata
     8701 ttctttttgc agacggtctt cgcgctcagc ttcgttaggc aggaagctat ttgcccaaat
     8761 ctgtccgtgc ggatgcgggt tagagcagcc catcgccgcg cctttgtttt caaagacctg
     8821 cacccacggg tatgttttcc ccagttctgc ggtttgctcc tgccaggttt tgacgatttc
     8881 cgtcaatgcc gcaacgctca gttctggcag cgttttactg tgatccggtg aaaagcagat
     8941 cacccggctg gtaccgcgcg cgctctggca acgcatcaaa ggatcgttgc tttctggcgc
     9001 atctggcgtg tcagacatca aggccgcaaa gtcattagtg aaaacgtaag tcccggtgta
     9061 atcggggttt ttatcgcctg tcacccgcac attacctgcg cagaggaagc aatctggatc
     9121 gtgcgcaggt aacacctgtt tggctggcgt ttcctgcgcc ccctgccagg ggcgcttagc
     9181 gcggtgcggt gaaaccagaa tccattgccc ggtgagcggg ttgtagcggc gatgtggatg
     9241 atcaacggga ttaaattgcg tcatggtcgt tccttaatcg ggatatccct gtggatggcg
     9301 tgactgccag tgccaggtgt cctgcgccat ttcatcgagt gtgcgcgtta cgcgccagtt
     9361 cagttcacgg tcggctttgc tggcgtccgc ccagtaggcc ggaaggtcgc ccgcgcgacg
     9421 tggtgcaaaa tgatagttaa ccggtttgcc gcaggcttta ctgaaggcat taactacgtc
     9481 cagcacgctg ctgcctacgc ctgcgccgag gttgtagatg tgtacgcctg gcttgttcgc
     9541 cagtttttcc atcgccacga cgtgaccatc tgccagatcc attacgtgga tgtaatcgcg
     9601 cacgccagtg ccgtcttcgg tcggataatc gttaccaaaa atcgccagtg agtcgcgacg
     9661 gcctacagca acctgggcaa tgtacggcat caggttattc ggaatgcctt gcggatcttc
     9721 gcccatatca cccgacggat gcgcgccaac cgggttgaag tagcgcagca gggcaatgct
     9781 ccagtccggt tgggcttttt gcagatcggt aaggatctgt tccaccatca gcttgctttt
     9841 gccgtaagga ctttgcggtg tgccggtcgg gaagctttca acatatggaa ttttgggctg
     9901 atcgccataa acggtggcgg aggagctaaa gataaagttt ttgacgttag cggcgcgcat
     9961 ggcgctaatc aggcgcagag taccgttgac attgttgtcg taatattcaa gcggtttttg
    10021 taccgattcg ccaacggctt tcagcccggc gaagtggatc acggtgtcga tagcgtgatc
    10081 gtgcaggatc tcggtcatca acgcttcgtt acggatatcg ccttcaacaa acgttggatg
    10141 tttgccgcct aaacgctcga taacaggcag tacgctgcgc ttactgttac agaggttatc
    10201 aagaatgatg acatcatgac cgttttgcag taattgcaca caggtatgac ttccaatgta
    10261 accgctacca ccggtaacca gaactctcat aattcgctcc attaggctta tggtatgaaa
    10321 taaccatagc ataacaaaga tgcgaaaagt gtgacatgga ataaattagt ggaatcgttt
    10381 acacaggaat ttagccgttt tttatgcgcg attaagtgat tataaaacag cggctttatg
    10441 aatgattgcg ctttttatct gaaaaaaaag acgcggtttc atgcctggat gcgtcaaacc
    10501 gttggccgga taacacgttt acgccgcctc cggcacggtc agcattaccg actcaattta
    10561 tttttgtcag cacatagcga tagagtccac cgtccggcac gaactcaaga cgatgggtaa
    10621 tacaggcagg cgcatcttca gcgtggtgcg aaacaaacaa caattgcgtt tcaccttcgc
    10681 taatcagcac atcaacaaaa cggcggataa gctggcgatt gagcggatca agcccctgta
    10741 gtggttcatc gagaataagc aacgtcggat gtttcaccag tgcgcggaca atcagcgcca
    10801 gacgctgctg tccccaggaa agactatgga aaggagcgtc agccgtgcgt ttatcaatgc
    10861 cgagaatatc cagccactgc tgcaccagtt tttgctggcg atccgaaacg gcctgataaa
    10921 tgccaatcga atcaaaatag ccagaaagaa tcacattacg cacagtagtg ctgacccggt
    10981 aatccagatg caaactactg ctgacgtaac cgatatgctt tttgatatcc cagatggttt
    11041 cgccgctgcc gcgacgtcgt ccgaaaagcg tcaaatcgtt gctgtaacct tgcggatgat
    11101 cgccagtaat caggcttaat aacgtcgatt ttcccgcgcc atttggcccg acaatttgcc
    11161 agtgttcgcc tggattcacc tgccagctaa ggttattaag gatggggcgt tcgttataag
    11221 aaaccacgcc attgttcagc acaatgcgcg gttcgttggc gggtaaggcg tgacgtgctg
    11281 aaggttcatc cggctccggc atttgcacac cttcaagcag ttcgctatgc gccagttgcg
    11341 caacgagtgc ttgttggagc agttcttctt tagcgccagt ttccgctaac gtgcaatccg
    11401 ccagcacgcc agcaaactgg acaaattccg ggatctcatc gaagcgattg agcaccagta
    11461 ccagagtaat accggactga tgtaacgagg cgagtagctc agccagatgc tgacgtgagg
    11521 caacatccag gccatcaaac ggttcatcaa ggatcaacaa gtcaggctcc gacatcagcg
    11581 cctgacacag cagggttttt cgcgtctcgc cagtggaaag gtatttaaag cgtcggtcga
    11641 ggagggcggt aataccgaac tgctgcgcca gttgcatgca acgcgaagta tcctttactt
    11701 catcctgaat gatctcagcc gtggtacgcc cggtgtcatc ttcgccagga ctaagcatat
    11761 cggtgttatt ccgctgccat tcgtcgctga cgagcttttg caattgctcg aaggagagac
    11821 gagtgatgtg ggaaaactgg ctttgccgtt cacctttcaa aagcggaagt tcccccgaca
    11881 gcgcgcgggc cagcgccgat ttcccgcttc cattcgaacc gacaaacgcc caactatcac
    11941 ccgcgtttaa cgttagccga ggcaattgca gcgtttttgt gtcgccaaga cgaaacgtgc
    12001 cttgcaaaat ttgcaacgat gacattttat atcccatttt gtgcagcgat tactgacagg
    12061 gatacgtgtt tcataacaaa ttgtcaacac gcttagcaca gcgtggcgat aatcacgctg
    12121 tcggcattaa agtaggccgt gacattctgc ccttgctgaa gagaagtcgc ttcatttacc
    12181 ggcactgtgg cgcacagtgt ttgcccgtcg ggtagcgcca ttaatacttc gcactgctct
    12241 gcgccgcgct caatatgact aataataccc ggtaattggt tgtcagcgtt ttgcgcgacc
    12301 gcctcgtcct gagtaatacc tacccacggc gcttttagca atatcaacac ttctttgcct
    12361 tcatccagcc ccagacgcgc gccgctttgt gcggtaattg cgactttcag gcgtgttttt
    12421 ccatcagcca gtaagacatc aacatgctgt tgaacgtcat catgatcgcg agcggtgatg
    12481 gtaccgaacc actggttacg ggcgctggtt tgcagtgaaa aacgtgagat cgcggccagc
    12541 aggctgttca gcggcagggc gtcatcgtca cttaacacat caaaggcttt ttgctggatt
    12601 tgcgccagta agtcatagag ttgaatcagt cgctgaccat agcgggtcag tactgcgccg
    12661 cctccacctt taccgcctgt tgcgcgctca accagaatat gctcacttaa ctgattcatc
    12721 tcgttaatgg catcccaggc gcttttatag ctgataccgg catctttcgc tccctggcta
    12781 atggaaccgg aaagcgcaat gtgttttagt agcgaaatgc ggcgcgggtc ggcgaataat
    12841 ttttgttgga gcttaagggt gagaaggatt tcggcctgca taacaatgtc ctggcaaaag
    12901 tcttattgtg acggaaaacg aacgccacgc aaagctgacc gcacaaaagg ggagtgcttt
    12961 tctgtgctta gcggttagaa tagtctcatg actatatctg gagttgacca tgttagagtt
    13021 attaaaaagt ctggtattcg ccgtaatcat ggtacctgtc gtgatggcca tcatcctggg
    13081 tctgatttac ggtcttggtg aagtattcaa catcttttct ggtgttggta aaaaagacca
    13141 gcccggacaa aatcattgat tccctgaatg cccgcttagt cgggcatttt ctttttctca
    13201 acttcctgct ttttctgccg atattttttc tcatctacct cacaaaggtt agcaataact
    13261 gctgggaaaa ttccgaactt gccgttatat tgacgcctac ataacgttag attaaggggt
    13321 taccaatggc tcgtaaatgg ttgaacttgt ttaccggggc ggcactctct ttcgctgttg
    13381 ctggcaatgc actggcagat gaagggaaaa tcacggtgtt cgccgccgca tcactgacta
    13441 acgcaatgca ggacattgct acgcagtata aaaaagagaa aggcgtggat gtggtttctt
    13501 ctttcgcttc gtcatctact ttggcccgtc agattgaagc gggtgcgcct gcggatctgt
    13561 ttatttctgc cgatcagaaa tggatggatt atgcggttga taaaaaagcg atcgatacgg
    13621 cttcgcgcca gacactgctc ggcaatagcc tggtcgttgt agcaccgaaa gccagcgagc
    13681 agaaagattt caccatcgac agcaaaacca actggacttc gctgctgaat ggcggtcgcc
    13741 tcgcggttgg tgatccggaa catgtccctg ctggcattta tgcgaaagaa gcactgcaaa
    13801 aactgggcgc atgggatacg ctttccccga aactggctcc ggcggaagat gttcgtggcg
    13861 cgctggcgtt ggtcgaacgc aatgaagcgc cgctgggtat tgtttacggt tctgacgcag
    13921 ttgccagcaa aggggtaaaa gtggttgcca ccttcccgga agattcacat aaaaaagtgg
    13981 aatatccggt tgctgttgtg gaagggcata acaatgcgac agtgaaagca ttttatgatt
    14041 atctgaaggg gccgcaggct gcggaaatct ttaaacgtta cggatttaca accaagtaat
    14101 gatactgacc gatccagaat ggcaggcagt tttattaagc ctgaaagttt cttccctggc
    14161 tgtgctgttt agcctgccgt ttgggatctt ttttgcctgg ttactggtac gttgcacgtt
    14221 tccgggcaaa gctctgctcg acagcgtact gcatctaccg ctggtgttac cgcccgtggt
    14281 cgtcggttac ctattattag tttcgatggg acggcgcgga tttatcggtg aacgtctgta
    14341 tgactggttt ggtctttctt tcgcgtttag ctggcgtggt gcggttctcg ctgccgctgt
    14401 catgtctttt ccgctgatgg tgcgggcgat tcgtctggcg ctggaaggtg ttgatatcaa
    14461 actggagcag gccgcaagaa cactgggggc cgggcgctgg cgcgttttct ttactatcac
    14521 gttaccgctg accttaccgg gaattattgt tggtacggta ctggcattcg ctcgttcgct
    14581 cggtgaattt ggcgcaacca tcacctttgt gtcgaacatt cctggtgaaa cgcggaccat
    14641 tccttctgcc atgtataccc tgatccagac ccctggcggg gaaagtggcg cggcgagact
    14701 gtgtattatt tctattgcgc tggcgatgat ctccctgttg atttcagaat ggctggccag
    14761 aatcagccgt gagcgggcgg ggcgctaatc atgctggaac tgaatttttc ccagacgttg
    14821 ggcaaccatt gcctgacgat taatgaaacg ctgcctgcca atggcatcac cgctattttt
    14881 ggtgtctctg gtgccggaaa aacctcgctg attaacgcca tcagtgggct gacacgtccg
    14941 caaaaagggc ggattgtcct caatggtcgg gtactaaatg acgccgataa aggtatctgc
    15001 ctgacgccag aaaagcgtcg cgtaggctat gtgtttcagg atgcgcggct gtttccgcat
    15061 tataaagtgc gtggcaattt gcgctacggc atggcgaaaa gcatggtcaa tcagttcgat
    15121 aagctggtgg cgcttttagg cattgaaccg ttgcttgatc gtttaccagg cagcctgtcc
    15181 ggaggcgaaa aacagcgcgt ggcgattggt cgggctttgc tgacagcacc ggaattgctg
    15241 ttgctggatg aaccgctggc atcactggat attccgcgta aacgcgaact gttgccttat
    15301 ctgcaacgtc tgacgcggga aatcaacatt ccaatgttgt atgtcagcca ttcgctggat
    15361 gagatcctcc atctggcaga cagagtgatg gtactggaaa acggtcaggt gaaagccttt
    15421 ggcgcgctgg aagaagtctg gggtagtagc gtgatgaatc cgtggctacc gaaagagcaa
    15481 caaagtagca ttctgaaagt gacagtactt gagcatcatc cgcattacgc gatgaccgcg
    15541 ctggcgctgg gcgatcagca tttgtgggtc aataagctgg acgaaccgct gcaagctgcg
    15601 ctacgcatcc gcattcaggc ttccgatgtc tcactggttt tacaaccgcc gcagcaaacc
    15661 agcattcgta acgtattgcg ggcaaaagtt gttaatagtt atgacgacaa cggccaggtg
    15721 gaagtggagc tggaagtcgg ctgtaaaacg ctgtgggcgc gtatcagccc gtgggccagg
    15781 gatgaactgg cgatcaaacc tggcctgtgg ctgtatgcgc aaattaaaag tgtgtcgata
    15841 accgcctgat taaatcaggt ggctataaat gaactgggca atgctgtcgg tggtgttatc
    15901 accaatcaca atgttggcgc gcgcttttac tgcgtcatcg gcgttgccca tcgccacgcc
    15961 tgtaccagcg gcttccagca tactaatatc attgaagtta tcgccgaacg ccacgacgtt
    16021 ttccatcgac cagccttgcg cctcaaccca tttcgtcaaa cgtttacctt tgctgttgcc
    16081 gccgcgtgca atatcaacct gatcgtgcca ggaccattca cactccagtc ccagttcatg
    16141 ttcgacatgc ttaccaaaat gctgcaattg cggcaggtca tcgtgcgtca gggcgaactt
    16201 ccatacggcg ttaacttgtt gcgccgtttc agccagagaa gcgacttgtg tgaaagtcgg
    16261 acgctgttcc ggcggcaggg tttgcgccca gttagacgtg cgaatgacat gtccggtcgg
    16321 gtgctcatag accatcgcat catccacata catcagaccg tgaatgtggt gttcattcag
    16381 catctcaatg agttgcaggg ctttattaac gggcattggg tccgcttcca gcacggtttt
    16441 tgcatgataa tcatacaaat aggtgccatt acagcaaata gcaggtgtat ccagcgccag
    16501 cgcctgataa aaaggatgaa tagcgacgtg atggcgacct gtgacgatga ttaatcgata
    16561 gcctgcttcg cgagcgcggg ccagggcttc tatcgatgat ggaagcaggg tctttttcgg
    16621 ggtcaataag gtgccgtcta agtcgagagc aatcacgcgt gtggtcatgg cgtattccag
    16681 attaaggtta agaattttcg tctgcgcgaa tggtacaccg ataccactat cggacaaaat
    16741 tctgcgtttt aattcagcat tcaccgccaa aagcgactaa ttttagctgt tacagtcagt
    16801 tgctaaatgc aaaggagcat tcatgaagca aacagtttat atcgccagcc ctgagagcca
    16861 gcaaattcac gtctggaacc tgaatcatga aggcgcactg acgctgacac aggttgtcga
    16921 tgtgccgggg caggtgcagc cgatggtggt cagcccggac aaacgttacc tctacgttgg
    16981 tgtccgtccg gagtttcgtg ttctggcgta tcgtattgca ccggatgatg gcgcgctgac
    17041 ctttgctgca gagtctgcgc tgccgggtag cccgacgcat atttccaccg atcaccaggg
    17101 gcagtttgtc tttgtcggtt cttacaatgc gggtaacgtg agcgtaacgc gtctggaaga
    17161 tggcctgcca gtgggcgtcg tcgatgtggt cgaggggctg gacggttgcc attccgccaa
    17221 tatctcaccg gacaaccgta cgttgtgggt tccggcatta aagcaggatc gcatttgcct
    17281 gtttacggtc agcgatgatg gtcatctggt ggcgcaggac cctgcggaag tgactaccgt
    17341 tgaaggggcc ggcccgcgtc atatggtatt ccatccaaac gaacaatatg cgtattgcgt
    17401 caatgagtta aacagctcag tggatgtctg ggaactgaaa gatccgcacg gcaatatcga
    17461 atgtgtccag acgctggata tgatgccgga aaacttctcc gacacccgtt gggcggctga
    17521 tattcatatc accccggatg gtcgtcattt atacgcctgc gaccgtaccg ccagtctgat
    17581 taccgttttc agcgtttcgg aagatggcag cgtgttgagt aaagaaggct tccagccaac
    17641 ggaaacccag ccgcgcggct tcaatgttga tcacagcggc aagtatctga ttgccgccgg
    17701 gcaaaaatct caccacatct cggtatacga aattgttggc gagcaggggc tactgcatga
    17761 aaaaggccgc tatgcggttg ggcaggggcc aatgtgggta gtggtcaacg cgcactaacc
    17821 gctgatttaa ccggcgcagt ctctcctgcg ccggtgtatt aacctatctc ctgtaacgca
    17881 tgtctctggc gttcgacgat attggtccac aaattgtctt tatcgtcagt ccacaaatta
    17941 atcagcaagg caaaaaagcg ttcagctgcc ggagaaagca cggcatcttt gcggcgaata
    18001 atccccaatg tccgacgtat aacgggttcc accagtggga taccaataat cgaagaatag
    18061 ggcgcatggg gcattgccag accaggaagc gccgaaatcc ccagtcccgc ctccaccagc
    18121 cctaatgacg tcgaaagatg acgcacttcg taaaaccagt ccagcttcca gggcttgtcg
    18181 gctagctgtt gttctatcaa cagtcggttg ccgctggagg agcgtacgcc aatcattttg
    18241 taacccacta actcctgcca ttctacaagc tgctttttgg ccaatggatg gtcacgccga
    18301 caggccagca cgaacggctc gttgaccagt ggggtaaaat caatggatga atttgtgacg
    18361 ttgttcatgt ttatgccaaa gtctgattcg ttacacagca ccgattccat gcaattattc
    18421 gtgccttgtt ccagaatccg cactttaata ttgggataca gctcattaaa tttaccgatt
    18481 gccaatggta aaaaataaaa taccgcggtc ggaatacacg ctaacgtcac catacctcga
    18541 tgataagcat tcatgtcgcg aatactaaaa agcgtttcat cgaatttttt tattaattcc
    18601 cgcgcctccg ggagcaacct ttttcctgct tttgttaacg taaccttgcg agttgtacgc
    18661 tcaaatagtt gaatgtgtaa atcttcctcc atctttttta ttcttcgcgt taatgcaggt
    18721 tgcgtaatat tgagtaattt agcagcattg ttaaatgaac tggactctgc cagtatgaca
    18781 aatgccttca tacttgataa ttcatgcttc attattactc cggaaaatgg aagcgacgat
    18841 tttgggtgac tggcagttaa aaattttaac tgcatttagc caacttaaat taatgaaaaa
    18901 atgttattaa tcgttgagc
//
LOCUS       DAHHTG010000085        18736 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.085, whole genome shotgun sequence.
ACCESSION   DAHHTG010000085 DAHHTG010000000
VERSION     DAHHTG010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18736)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18736)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18736
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.085"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            118..324
                     /locus_tag="NBI74_003040"
     CDS             118..324
                     /locus_tag="NBI74_003040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbfA family protein"
                     /protein_id="HCD1933332.1"
                     /translation="MELYKEYPAWLIFLRRTCAVAAGVLALPFMLFWKDRARFYSYLH
                     RVWSKTSDKPVWMDQAEKATGDFY"
     gene            425..934
                     /locus_tag="NBI74_003041"
     CDS             425..934
                     /locus_tag="NBI74_003041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgA family protein"
                     /protein_id="HCD1933333.1"
                     /translation="MNQQLFDDSTLIRIFALHELHNLKDRGLTRGALLDYHSRYKLVF
                     LAHSQPEYRKLGPFVADIHRWQSLDDYYNQYRQRVIVLLSHPANRRDHTNVLMHVQGY
                     FRPHIDSTERQQLAALIDSYRRGEQSLLAPLMRIKHYMALYPDAWLSGQRYFELWPRV
                     INLRHAGVL"
     gene            931..2349
                     /gene="phrB"
                     /locus_tag="NBI74_003042"
     CDS             931..2349
                     /gene="phrB"
                     /locus_tag="NBI74_003042"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HCD1933334.1"
                     /translation="MTTHLVWFRQDLRLHDNLALAAACRNSSARVLALYIATPRQWAA
                     HNVSPRQAELINTQLNALQNALAEKGIPLLFREVDDFAASVEIVKQVCAENRVTHLFY
                     NYQYEVNERARDVEVERALRNVVCEGFDDSVILPPGAVMTGNHEMYKVFTPFKNAWLK
                     RLREGMPECVAAPKVRSSGAIDPAPSITLNYPRQSFDTAHFPVEEKAAIAQLRQFCQN
                     GAGEYEQQRDFPAVEGTSRLSASLATGGLSPRQCLHRLLAEQPQALDGGAGSVWLSEL
                     IWREFYRHLMTYYPSLCKHRPFIAWTDRVQWQSNPAHLKAWQEGKTGYPIVDAAMRQL
                     NSTGWMHNRLRMITASFLVKDLLIDWREGERYFMSQLIDGDLAANNGGWQWAASTGTD
                     AAPYFRIFNPTTQGEKFDREGEFIRQWLPELRDVPGKAVHEPWKWAEKTGVTLDYPQP
                     IVDHKEARLRTLAAYEEARKGA"
     gene            complement(2391..3872)
                     /gene="dtpD"
                     /locus_tag="NBI74_003043"
     CDS             complement(2391..3872)
                     /gene="dtpD"
                     /locus_tag="NBI74_003043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide permease DtpD"
                     /protein_id="HCD1933335.1"
                     /translation="MNKHASQPRAIYYVVALQIWEYFSFYGMRALLILYLTNQLKYSD
                     NHAYELFSAYCSLVYVTPILGGFLADKVLGNRMAVMLGALLMAIGHVVLGASEIHPSF
                     LYLSLAIIVCGYGLFKSNVSCLLGELYEPTDPRRDGGFSLMYAAGNVGSIIAPIACGF
                     AQEEYSWAMGFGLAAVGMIAGLVIFLCGNRHFTHTRGVNKKVLRATNFLLPNWGWLLV
                     LLVATPALITVLFWKEWSVYALIVATIIGLGVLAKIYRKAENQKQRKELGLIVTLTFF
                     SMLFWAFAQQGGSSISLYIDRFVNRDMFGYTVPTAMFQSINAFAVMLCGVFLAWVVKE
                     SVAGNRTVRIWGKFALGLGLMSAGFCILTLSARWSAMYGHSSLPLMVLGLAVMGFAEL
                     FIDPVAMSQITRIEIPGVTGVLTGIYMLLSGAIANYLAGVIADQTSQASFDASGAINY
                     SINAYIEVFDQITWGALACVGLVLMIWLYQALKFRNRALALES"
     gene            4143..4886
                     /gene="ybgI"
                     /locus_tag="NBI74_003044"
     CDS             4143..4886
                     /gene="ybgI"
                     /locus_tag="NBI74_003044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000798886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radiation resistance protein YbgI"
                     /protein_id="HCD1933336.1"
                     /translation="MKNTELEQLINEKLNSAAISDYAPNGLQVEGKEMVQKIVTGVTA
                     SQALLDEAVRLGADAVIVHHGYFWKGESPVIRGMKRNRLKTLLANDINLYGWHLPLDA
                     HPELGNNAQLAALLGITVMGEIEPLVPWGELTMPVPGLELASWIEARLGRKPLWCGDT
                     GPEVVQRVAWCTGGGQSFIDSAARFGVDAFITGEVSEQTIHSAREQGLHFYAAGHHAT
                     ERGGIRALSEWLNENTDLDVTFIDIPNPA"
     gene            4909..5565
                     /gene="pxpB"
                     /locus_tag="NBI74_003045"
     CDS             4909..5565
                     /gene="pxpB"
                     /locus_tag="NBI74_003045"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HCD1933337.1"
                     /translation="MQRARCYLIGETAVVLELEPPVTLASQKRIWRLAQRLVDMPNVV
                     EAIPGMNNITVILRNPESLALDAIERLQRWWEESEALEPESRFIEIPVVYGGAAGPDL
                     AVVAAHCGLSEKQVVELHSSVEYVVWFLGFQPGFPYLGSLPEQLHTPRRAEPRLHVPA
                     GSVGIGGPQTGVYPLATPGGWQLIGHTSLSLFDPACDEPILLRPGDSVRFVPQKEGVC
                     "
     gene            5559..6491
                     /gene="pxpC"
                     /locus_tag="NBI74_003046"
     CDS             5559..6491
                     /gene="pxpC"
                     /locus_tag="NBI74_003046"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpC"
                     /protein_id="HCD1933338.1"
                     /translation="MLKIIRAGMYTTVQDGGRHGFRQSGISHCGALDMPALRIANLLV
                     GNDANAPALEITLGQLTVEFETDGWFALTGAGCEARLDDNAVWTGWRLPMKAGQRLTL
                     KRPQHGMRSYLAVAGGIDVPPVMGSCSTDLKVGIGGLEGRLLRDGDRLPIGKAKRDFM
                     EAQGVKQLLWGNRIRALPGPEYHEFDRASQDAFWRSPWQLSPQSNRMGYRLQGQILKR
                     TTDRELLSHGLLPGVVQVPHNGQPIVLMNDAQTTGGYPRIACIIEADMYHLAQIPLGQ
                     PIHFVQCSLEEALKARQDQQRYFEQLAWRLHNEN"
     gene            6481..7215
                     /gene="pxpA"
                     /locus_tag="NBI74_003047"
     CDS             6481..7215
                     /gene="pxpA"
                     /locus_tag="NBI74_003047"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HCD1933339.1"
                     /translation="MKIDLNADLGEGCASDAELLTLVSSANIACGFHAGDAQTMQACV
                     REAIKNGVAIGAHPSFPDRENFGRSAMQLPPETIFAQTLYQIGALAAITRAQGGVMCH
                     VKPHGMLYNQAAKEAQLADAIARAVYACDPALILVGLAGSELIRAGERYGLVTREEVF
                     ADRGYQADGSLVPRSQPGALIENEEQALAQTLEMVQHGRVKSITGEWAMVTAQTVCLH
                     GDGEHALAFARRLRATFAEKGIVVAA"
     gene            7251..8042
                     /gene="nei"
                     /locus_tag="NBI74_003048"
     CDS             7251..8042
                     /gene="nei"
                     /locus_tag="NBI74_003048"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease VIII"
                     /protein_id="HCD1933340.1"
                     /translation="MPEGPEIRRAADNLEAAIKGKPLTDVWFAFPQLKSYQSRLIGQH
                     VTHVETRGKALLTHFSNDLTLYSHNQLYGVWRVVDTGEEPQTTRVLRVKLQTADKTIL
                     LYSASDIEMLTPEQLTTHPFLQRVGPDVLDPNLTPEVVKERLLSPRFRNRQFAGLLLD
                     QAFLAGLGNYLRVEILWQVGLTGNHKAKDLNAPQLDALAHALLDIPRLSYATRGQVDE
                     NKYHGALFRFKVFHRDGEPCERCGGIIEKTTLSSRPFYWCPGCQH"
     gene            complement(8039..9085)
                     /locus_tag="NBI74_003049"
     CDS             complement(8039..9085)
                     /locus_tag="NBI74_003049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415243.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HCD1933341.1"
                     /translation="MPVLQWGMLCVLSLLLSIGFLALHLPAALLLGPMIAGIIFSMHG
                     VTLQLPRSAFLAAQAILGCMIAQNLTGSILTTLAVNWPIVLAILLVTLLSSAIVGWLL
                     VRYSSLPGNTGAWGSSPGGAAAMVAMAQDYGADIRLVAFMQYLRVLFVAGAAVLVTRM
                     MLGDNAEAVNQQIVWFPPVSINLLLTILLAVVAGTAGCLLRLPSGTMLIPMLAGAVLQ
                     SGQLITIELPEWLLAMAYMAIGWRIGLGFDKQILLRALRPLPQILLSIFALLAICAGM
                     AWGLTRFMHIDFMTAYLATSPGGLDTVAVIAAGSNADMALIMAMQTLRLFSILLTGPA
                     IARFISTYAPKRSA"
     gene            complement(9265..9978)
                     /locus_tag="NBI74_003050"
     CDS             complement(9265..9978)
                     /locus_tag="NBI74_003050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HCD1933342.1"
                     /translation="MSESALLTSVTLSSQVKNHILNLIAEQKLTPGMPVPSEVQLIQK
                     LGVSRGIVREAYRSLAALGILETHSGKVPRIKKFDDEVLRVVFGFALATEQVTTEQIL
                     QVRRWLELGVVEAAARNGEEEDFANLRAEMAKIREQFNDVESFITHDIRFHQLLAVAS
                     KNPLFMMLLQALHQQLRQSIQEGLNAQSDQKEYGNHIITLHQKICDAVCARDVETATQ
                     AMKSHFDSPVSALINNSSS"
     gene            10242..10928
                     /locus_tag="NBI74_003051"
     CDS             10242..10928
                     /locus_tag="NBI74_003051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000265972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RraA family protein"
                     /protein_id="HCD1933343.1"
                     /translation="MWKSDNELFALMKTRLFTAVVGDILDTLGYMHQFVSPAIKPLDV
                     NTVVAGRAMPVLESDVYETKSTSGKTAIGEKPFGIMFEALDALKENDVYITTGSSLRY
                     ALWGGLMSTRAMHLKAAGAVLDGYARDSAEILELKFPTFCHGTYAQDQGPRGKVIDFG
                     VPIEFHGVRVNPGDIVFGDRDGLLIIPQVVEKEVITQALEKVATESEVRKAISDGMST
                     VQAFETFGVM"
     gene            10941..11954
                     /locus_tag="NBI74_003052"
     CDS             10941..11954
                     /locus_tag="NBI74_003052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000646083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HCD1933344.1"
                     /translation="MKALTIEQGSQARFVDIPEPVCGKGEVLINVERVGLCGSDLNTW
                     RGLNPLVNYPCIPGHEIGGTVLATGDMAGAIKPGDRVVVLPCTECGVCSSCLSGRPNA
                     CKNNQTLGVQRQGGMAQQLTMPAHKVIVCNALSVDELALVEPLAVGLHAARRAQINVG
                     EWVAVIGCGVIGLGAIAAASAMGARVIAIDIDDRKRNIALECGAQRFLNSQQCDVAAE
                     LVLLNDGRGPEVVIEAVGNQQTIEQATGLVAFSGRVVYVGYAKQPVSYQSAQFLMKEL
                     DIRGSRNATVADFRAVLALMESGRYPVSNLVSAHYPLEQADHAFSDWASDPGAYTKIL
                     IDL"
     gene            11966..12721
                     /locus_tag="NBI74_003053"
     CDS             11966..12721
                     /locus_tag="NBI74_003053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000991076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCD1933345.1"
                     /translation="MNAFSLHNKTALITGAASGLGLAMAKCMIVSGAKVIIADLNGEL
                     AQATAGTLGSQASWIQMDVGDTAAAQEKVDVLISEHGAIDILVNNAGNHCKKPIEEMS
                     VSDFESVLDVHVVGAFALTKALVPHMKQRGKGCVLFTASMTSFLGQPLVTGYSAAKSA
                     YLGMIRGMTTELAVHGIRVNGIAPGWIDTPMLRKAIEGDDERRNKILGRTPMKKFGKP
                     EDIGWAAVYLASDAAAFVSGQVLVVDGGALIGF"
     gene            12736..13047
                     /locus_tag="NBI74_003054"
     CDS             12736..13047
                     /locus_tag="NBI74_003054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002432396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HCD1933346.1"
                     /translation="MRKRALKLHAKDNCVVALNDIASGDIVWWDEGEMVACSSVTLGH
                     KLACEALNNGDSILKYGAIIGHATRDIAMGEHVHSHNLASNAIAIFHHDDAGYQSEEK
                     A"
     gene            13047..14243
                     /locus_tag="NBI74_003055"
     CDS             13047..14243
                     /locus_tag="NBI74_003055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000399432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HCD1933347.1"
                     /translation="MEGYVRQNGRPGIRNTVQVIFMVECASHVAKKIAEGFPCHDVQY
                     FGFSGCYPSDYGYRLVKNLATHSNVGGVLIISLGCENFDRRRLEQEVRESGRPCHTLV
                     IQENKGTTNTITLGKQLVAEMLEQLADTPRCILNWSDLVVGTICGGSDGTSGITGNPA
                     VGRAFDQLLEKGATCIFEESGELLGCEQHMMSRAASSQARDAIEVAMTKAERYYSLMG
                     LGSFSNGNAEGGLTTQEEKSLGAYTKSGDALIAGVILPTQQTPFPGLWLMDVVPDGEP
                     RFGYPNINDSSEALELIASGCHMVLFTTGRGSVIGSVIAPIIKVCNNPQTWEHLSDDM
                     DVNAGRVISEGATLDDIADDIMQVIEHVANGQYCAAERLEHQEFVLTYKAFDYSKSAV
                     CRADPR"
     gene            14271..15239
                     /locus_tag="NBI74_003056"
     CDS             14271..15239
                     /locus_tag="NBI74_003056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate TRAP transporter
                     substrate-binding protein"
                     /protein_id="HCD1933348.1"
                     /translation="MKKLLTPLLFLLALFSPMLNAAPVTIKIAYESNPGEPADTVVKH
                     WAELVKKKSNGDIVFELYPSSQLGSKQDITEQALMGMNIITISDVGFLTDYDADLGIL
                     FGPYLTEDPQKLFAIYASDWFKQKEQKLREKGVHIVMSNYLYGTRQIIAKKPIRTPED
                     LKGMKIRVPNNVMQIKAIEAMGATPTPMPLGDVYPALTQGMIDGVENPVSVLYGQKLH
                     EQAKYLSMVNYLTNTSLWLGGEAFFSTLTPAQLDIIHQTAYEAGLYSQKITTEQDATM
                     LKTMQEQGVEIIYPDVAPFKEKALKVYQQFPEWTPGLYDTIQQQLK"
     gene            15299..15760
                     /locus_tag="NBI74_003057"
     CDS             15299..15760
                     /locus_tag="NBI74_003057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001237778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HCD1933349.1"
                     /translation="MRLLGKMIEVAAVLTLFILVIMTIAAVIMRYCFHMPLQWTEEVS
                     GLLMIWVVMFGAVVAERDNTHLSIPFLADALTSRGQRILAFLVSLMSIILLVVMAWSA
                     WKLAAGTAFKTTQILKISWFWIDIAVTVGAIGVACFTALQLIKKTPRKEGE"
     gene            15760..17070
                     /locus_tag="NBI74_003058"
     CDS             15760..17070
                     /locus_tag="NBI74_003058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000126139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HCD1933350.1"
                     /translation="MTALSELLAGSWLDNWLMIIPLMFVCLFINMPVWLAMFCGILTY
                     FLCYSNVPLMVAIQRLIATTQTPSLLAIPFFILLGTLMSYTGIAARILQIANVLIGRM
                     RGGLGGANILVSTMMGGVSASNLADAAMLSRMMVPEMEQKGYSRGFAAAITASGSLIT
                     PIIPPGIALILYGLVADVSIGSMFMAGILPGLLCSAMLIVTVWLVAIRRGLKPSSEHW
                     PKPKEVSVALVQAWPALFLIVAVIGGIRANIFTPTEAGAVAVLLVMFIGFVIYRELKI
                     SHVFTALSETARSTASVMLIIMASAALGWIFSLERAGITVAEFVTSLTDNKYVFLLII
                     NLLLLVLGMFLEGSAILLILVPLLKPVVAHFGIDPVHFGIIMILNLSLGTLSPPVGTV
                     MLLVCNTVQVSVGQFMREAVPLLISLILLLLVITYIPAISLLLV"
     gene            complement(17117..18400)
                     /gene="gltA"
                     /locus_tag="NBI74_003059"
     CDS             complement(17117..18400)
                     /gene="gltA"
                     /locus_tag="NBI74_003059"
                     /EC_number="2.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (Si)-synthase"
                     /protein_id="HCD1933351.1"
                     /translation="MADTKAKLTLNGDTAVELDVLKGTLGQDVIDIRTLGSKGVFTFD
                     PGFTSTASCESKITFIDGDEGILLHRGFPIDQLATDSNYLEVCYILLNGEKPTQEQYD
                     EFKTTVTRHTMIHEQITRLFHAFRRDSHPMAVMCGITGALAAFYHDSLDVNNPRHREI
                     AAFRLLSKMPTMAAMCYKYSIGQPFVYPRNDLSYAGNFLNMMFSTPCEPYEVNPILER
                     AMDRILILHADHEQNASTSTVRTAGSSGANPFACIAAGIASLWGPAHGGANEAALKML
                     EEISSVKHIPEFVRRAKDKNDSFRLMGFGHRVYKNYDPRATVMRETCHEVLKELGTKD
                     DLLEVAMELENIALNDPYFIEKKLYPNVDFYSGIILKAMGIPSSMFTVIFAMARTVGW
                     IAHWSEMHSDGMKIARPRQLYTGYAQRDFKSDIKK"
     gene            complement(18606..18713)
                     /locus_tag="NBI74_003060"
     CDS             complement(18606..18713)
                     /locus_tag="NBI74_003060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1933352.1"
                     /translation="MRKSYEVGISPKINLCNSVEVLTNSFGTVISGRQV"
BASE COUNT         4594 a         4407 c         5120 g         4615 t
ORIGIN      
        1 aaataagtgg tcggatgagt agtaaaatta cacaaacagc ggtactattt tcatcagata
       61 aacgaattca tttttccggt gctattcacc gggtagatac aaaaggggga gaaaagtatg
      121 gaactctaca aagaatatcc tgcatggctt atctttttac gccgtacttg tgcggttgca
      181 gcgggcgttc tggcgctgcc tttcatgctt ttctggaaag accgcgcccg cttttatagc
      241 tacctgcacc gcgtctggtc gaaaaccagc gataaaccgg tgtggatgga tcaggccgaa
      301 aaggcaaccg gtgattttta ctgattgcca aacatgacgt ttaaagaaaa ccgccggtgg
      361 aaacgctggc ggttttcttt tttcggcgtc tacacttact cctgtaaacc gttcaggagc
      421 agtaatgaat caacaacttt ttgatgacag cacccttatc cgcatttttg ctttgcatga
      481 gttacacaat ctcaaagatc gaggactaac gcgcggggcg cttctcgatt atcacagccg
      541 ctataaactc gtctttctgg cgcattctca gccggagtac cgcaaacttg gcccgtttgt
      601 ggctgatatt caccggtggc aaagtctgga tgactattac aaccagtacc gccaacgcgt
      661 gattgttttg ctttctcacc ctgccaaccg gcgtgatcat actaacgttt tgatgcacgt
      721 tcagggatat tttcgcccac atattgattc cacagaacgc cagcagctgg cggcgcttat
      781 cgacagttat cgccgtggcg agcaatcgct tcttgcgccg ctgatgcgta tcaaacacta
      841 tatggcgctt tatcctgacg cctggctttc agggcagcgt tatttcgaac tttggccgcg
      901 tgtgattaac ttgcgccatg caggagtttt atgactaccc atctggtctg gtttcgccag
      961 gatttacgtc tgcacgataa tctcgcactg gctgccgcct gccgcaattc gtctgcacgc
     1021 gtgctggcgt tgtatatcgc taccccacgc cagtgggcgg cgcataatgt gtcgccgcgt
     1081 caggctgaac ttatcaatac acaacttaat gcgctgcaaa acgcgctggc ggaaaaaggt
     1141 attcctttat tgttccgtga agtggatgac tttgccgcca gcgtcgaaat agttaaacag
     1201 gtgtgcgcgg aaaaccgcgt tactcatctt ttttataact atcagtatga ggtaaatgag
     1261 cgggcgcggg atgtggaagt tgaaagagcg ctgcgtaacg tggtgtgtga aggatttgat
     1321 gacagcgtga tcctgccgcc gggggcggtg atgactggca atcatgagat gtacaaagtc
     1381 tttacgcctt ttaagaatgc ctggctgaaa cggctgcggg aagggatgcc ggagtgcgtc
     1441 gctgcgccaa aagtacgtag tagtggagcg atagatcccg cgccatccat tacgctgaat
     1501 tatcctcgtc agtctttcga tactgcgcat ttcccggtgg aagaaaaagc ggcgattgcg
     1561 caattacgcc agttttgcca gaacggtgcc ggagagtatg agcaacaacg agattttccg
     1621 gcagtagaag gcaccagtcg tttgtccgcc agcctggcaa cgggcgggtt atcgcctcgc
     1681 cagtgtttgc atcgcttgtt ggcggaacag ccgcaggcgc tggacggtgg agccggtagt
     1741 gtctggctta gtgagctgat ctggcgcgag ttctaccgcc atttgatgac gtattatcct
     1801 tcgctgtgta aacatcgtcc gtttattgcc tggacggatc gtgtacagtg gcaaagtaat
     1861 cccgcccatt taaaggcctg gcaggaaggc aaaacgggat acccgattgt cgatgctgcc
     1921 atgcgtcagc ttaacagcac tggctggatg cataaccggc tacggatgat tacagccagt
     1981 tttctggtga aagatttgtt gatcgactgg cgcgaaggcg agcgatattt catgtcgcag
     2041 ctgattgatg gtgatttggc agctaataac ggtggctggc agtgggccgc ttctacgggt
     2101 actgatgcag cgccgtattt tcgtattttc aacccgacaa ctcagggcga gaaatttgac
     2161 cgcgaaggcg aatttatccg ccagtggcta ccggagctgc gcgatgtacc ggggaaagcg
     2221 gtgcatgagc cgtggaagtg ggcagagaaa acaggtgtga cgttggatta tccgcaaccg
     2281 atagtcgatc acaaagaggc aaggctgcga acgctggcag cgtacgaaga agcgaggaaa
     2341 ggagcctgat aaaacgcagc gagcgtttta tcaggcatgg tgctggctta ttaagactcc
     2401 agcgccagcg cgcggtttct gaacttaagc gcctgataca gccaaatcat cagtaccagt
     2461 cctacacacg ccagtgcgcc ccaggtaatt tgatcaaata cttcaatata tgcattgatg
     2521 gagtagttga tcgccccgga agcatcaaac gaagcctgcg atgtctgatc ggcaatcacg
     2581 cccgccagat agttcgcaat cgcgccggaa agcagcatat aaatgccggt taatacgccg
     2641 gtcacgccgg ggatttcaat gcgcgtaatc tgcgacatag caaccgggtc gataaacagt
     2701 tccgcaaagc ccatcaccgc caggcctaac accatcagtg gcagagaaga gtgaccatac
     2761 attgcggacc agcgggcgct taaggtcaga atgcagaatc cggcgctcat caggccaagg
     2821 ccaagagcaa attttcccca gatgcgcacg gtacgattac ctgcgacgct ttctttaacc
     2881 acccacgcca ggaacacacc gcacagcata actgcgaagg cattaatcga ctggaacata
     2941 gcagtcggaa cggtataacc aaacatatcg cggttaacga agcggtcgat ataaaggcta
     3001 atcgagctac ccccctgttg tgcgaatgcc cagaacaaca tgctgaagaa ggtgagggta
     3061 acaatcagcc ccagctcctt acgctgtttt tggttttctg ctttgcgata aatttttgcc
     3121 agaaccccaa ggccaatgat cgtcgcgaca atcaaggcgt ataccgacca ctctttccag
     3181 aacaacacgg taatcagcgc aggcgttgcc accagcagaa ccagtagcca tccccagttg
     3241 ggcaggagaa agtttgtcgc acgcagtacc tttttgttaa cgccgcgggt atgtgtgaaa
     3301 tgacgattgc cacataagaa aatgaccaga cccgcgatca tacccaccgc tgccaggcca
     3361 aagcccatcg cccaactgta ctcttcctgg gcgaaaccac aggcgatagg tgcgataata
     3421 gaccccacgt tacccgccgc atacatcagc gagaaaccgc catcacgacg tggatcggtt
     3481 ggctcataca gctcaccgag caggcaactg acgttggatt taaacagacc atagccgcag
     3541 acgataatcg ccagggacag atagaggaat gacggatgga tctcactggc acccagcacc
     3601 acatgaccga tcgccatcaa caacgccccc agcatcaccg ccatgcgatt gccgagaact
     3661 ttatccgcca aaaagccacc gaggattggc gtgacatata ccagcgaaca gtaggcgcta
     3721 aataactcgt aggcgtgatt atcgctatat tttagttgat tggtgagata gaggatcagc
     3781 agggcacgca tgccgtaaaa gctaaaatat tcccagattt gcagcgcaac gacatagtaa
     3841 atagcgcgcg gttgtgatgc gtgtttattc ataataattc cacaattgac tgcaacgtca
     3901 cgtaatagtt gcgcggcgtg cttgtgtttc cttaaagtta aaactttgat acagatctga
     3961 ttatttatgc aatatgctgt ctgattgcat aaatatacat tagctgaagc gtgatgatat
     4021 aaacaatttg cgtcttttgg cgtcgggttt gtttcttaat atgtcaaaag attggctatc
     4081 gacgaacagg cgaggtaacg ctatgttaac ggtgtagcta tcagaatacg gaaggcgcga
     4141 agatgaaaaa caccgaactg gaacaactga ttaacgaaaa actgaacagc gcggcgatta
     4201 gtgattacgc gccgaatggt ttgcaggtgg aaggcaaaga gatggtgcaa aaaattgtta
     4261 ccggtgtaac tgccagccag gctttgctcg atgaggcagt gcgtctgggc gcggatgcgg
     4321 tcattgtgca tcatggctac ttctggaaag gtgaatctcc ggtcattcgc ggaatgaagc
     4381 gtaaccgttt aaaaacgttg ctggcgaatg atatcaacct gtatggctgg catctgcccc
     4441 tggacgcgca tccggagctg ggtaataacg cacagctggc ggcgttactg gggatcacgg
     4501 tcatgggcga aattgagccg ttggtgccgt ggggcgaact gaccatgcct gtgccgggac
     4561 tggaactggc ctcctggatt gaagcgcgtc tgggacgtaa gccgttatgg tgtggcgata
     4621 ccggacctga ggtggtccag cgcgtcgcct ggtgcacggg gggtgggcaa agttttatcg
     4681 atagcgccgc gcgttttggc gtggatgctt ttattactgg cgaagtttct gaacagacca
     4741 ttcattcagc ccgcgagcag ggattgcatt tttatgctgc gggtcaccat gccactgaac
     4801 gcggtggtat tcgcgcattg agcgagtggc tgaatgaaaa taccgatctt gatgtgacct
     4861 ttattgatat tcctaatcct gcataacgaa taatcagagg gatcgaaagt gcaacgagcg
     4921 cgttgttatc tgataggtga aacggcggta gtactggaac tggaaccgcc ggtgacgctg
     4981 gctagccaga aacggatctg gcgactggca cagcgtctgg tggatatgcc gaatgtggtt
     5041 gaagccattc ctggcatgaa caatatcaca gtgattttgc gtaatcctga gtcgctggcg
     5101 ctggatgcca tagagcgttt gcaacgctgg tgggaggaga gtgaggcgct ggagccggag
     5161 tctcgcttta ttgaaattcc ggtagtttac ggtggcgcag ccggaccgga tttggcggtg
     5221 gtcgcggcgc attgtggatt gagcgaaaaa caggttgttg aattgcactc ctccgtggaa
     5281 tacgtggtct ggtttttagg ttttcaaccg ggcttcccgt atctcgggag tttgccggaa
     5341 caactacaca cgccacggcg cgctgaaccg cgcttgcacg ttccggcagg ttctgtcggg
     5401 atcggcgggc cgcagaccgg tgtttatccg ctggcaacgc cgggtggctg gcagttgatt
     5461 ggtcatacct cactcagcct gtttgatccg gcgtgtgacg aacccatctt attacgtccg
     5521 ggagacagcg tgcgctttgt accgcagaag gagggagtat gctgaagatt attcgtgcgg
     5581 gcatgtacac caccgtgcag gatggcggcc gtcacggctt tcgtcagtct ggtatcagcc
     5641 actgtggcgc actggatatg cctgcgttac gcattgctaa cctgctggtg ggtaatgacg
     5701 ccaatgcccc cgcgctggag atcacgctcg gtcagttaac ggttgagttc gaaactgatg
     5761 ggtggtttgc tctgacgggt gccggttgcg aagcgcggct ggatgataat gccgtctgga
     5821 ccggctggcg attgccgatg aaagcaggcc agcgtttaac gcttaaacgc ccgcagcacg
     5881 ggatgcgcag ttatctggcg gtcgcgggtg gtattgatgt tccgccggta atgggatcat
     5941 gcagcaccga tctcaaagtg gggattggcg ggctggaagg ccgtttactg agggatggtg
     6001 accgactccc gattggcaaa gcgaagcgtg attttatgga agcgcagggc gttaaacagc
     6061 tgctctgggg caaccgcatt cgcgccttgc cggggccgga atatcatgag ttcgatcgcg
     6121 cctcgcagga tgcattctgg cgttcgccct ggcagcttag cccgcaaagt aaccgcatgg
     6181 gctatcgctt acaggggcaa attttaaaac gcaccaccga tcgcgaactg ttatctcacg
     6241 gtttgttgcc gggcgtggtg caggtgccgc ataacggcca gccgattgta ttgatgaacg
     6301 acgcacagac caccggtggt tacccgcgta ttgcctgtat cattgaggct gatatgtacc
     6361 atctggcgca aattccgctc ggtcagccga ttcattttgt ccagtgttca ctggaagagg
     6421 cactgaaagc gcggcaagat cagcaacgtt atttcgaaca attagcgtgg cggctgcaca
     6481 atgaaaattg acctgaacgc cgatctgggc gaaggctgcg ccagcgacgc agagctatta
     6541 acgctggttt cctctgccaa tattgcctgt ggatttcatg caggagatgc gcaaaccatg
     6601 caggcttgcg tgcgtgaagc aataaaaaat ggtgtcgcga ttggcgctca cccgagtttt
     6661 cccgacaggg aaaattttgg tcgcagcgcc atgcagctgc cgccagaaac cattttcgcc
     6721 cagacgctgt atcaaattgg cgcgctggca gcgattaccc gtgcgcaagg cggcgtaatg
     6781 tgtcatgtca aaccgcacgg catgttatac aaccaggcgg cgaaagaggc acaactggca
     6841 gacgctatcg ccagagcggt atacgcttgc gatccggcat tgattctcgt cgggctggca
     6901 ggaagcgagc tgattcgtgc aggcgaacga tatggcctgg taacgcgcga ggaagtgttt
     6961 gccgatcgtg gttatcaggc agacggttcg ctggtgccgc gaagccagcc aggcgcgttg
     7021 attgaaaacg aagaacaggc gctggcgcaa acgttggaga tggtgcaaca cggcagagtc
     7081 aaaagtatca ccggcgaatg ggcaatggtc acggcgcaaa cggtctgcct acatggcgat
     7141 ggcgagcacg cactggcgtt cgcccgccga ttacgagcta catttgctga aaaggggatt
     7201 gttgtcgcag cataaccccg cttaataaag aatgaaaaaa ggatatcacc atgcctgaag
     7261 gcccggagat ccgccgtgca gcggataacc tggaggcggc gatcaaaggc aaaccactaa
     7321 ctgatgtctg gtttgccttc ccgcagttaa aatcttatca atcacgactt atcggtcaac
     7381 acgttaccca tgtggaaacg cgtggtaagg cgttgttaac tcatttttcc aacgacttaa
     7441 cgctctacag ccataatcag ctttacggcg tctggcgcgt ggttgatacc ggcgaagagc
     7501 cgcagaccac gcgagtattg cgggtaaaac tgcaaacggc tgacaaaacc attctgcttt
     7561 atagcgcctc ggatattgag atgttgactc cggaacaact gaccacgcat ccgtttttac
     7621 aacgcgttgg tcccgatgtg ctggatccga atctgacgcc ggaggtggta aaggaacgat
     7681 tattgtcgcc gcgctttcgt aaccgtcagt ttgctggatt actgctcgat caggcgtttc
     7741 tggcaggact tggcaattat ttgcgggtgg agatcctctg gcaggttggg ttgaccggaa
     7801 atcataaagc gaaagatctc aatgcgccgc aactggatgc actcgcacac gcgttactgg
     7861 atattccgcg actttcttac gctacgcggg ggcaggtgga tgaaaacaaa tatcatgggg
     7921 cgctgtttcg ctttaaggtt tttcatcgtg atggcgaacc ttgcgaacgc tgtggcggca
     7981 tcattgagaa aaccacgctg tcatcgcgcc cgttttactg gtgcccaggc tgccagcact
     8041 aggccgaacg cttcggcgca taggttgaaa taaaccgtgc gatggcaggc cccgtcagca
     8101 aaatactgaa caggcgcagg gtttgcatcg ccatgatgag cgccatatcg gcattgctcc
     8161 ctgcggcgat gaccgccacg gtatcaagcc cgccggggct ggtggcgagg taggcggtca
     8221 taaaatcaat atgcataaac cgggtcagcc cccacgccat gcccgcacaa atagccagca
     8281 gagcaaaaat cgacagcagg atttgcggta gcgggcgcaa tgcccgcagt aatatttgct
     8341 tatcgaaacc aagaccaatc cgccagccaa ttgccatata tgccatcgcc agcagccatt
     8401 ccgggagttc aatcgtgatg agctgaccag actggagcac tgcgcccgcc agcatcggga
     8461 tgagcatcgt gccggaagga agacgtagca agcatcccgc cgtaccggca acgaccgcga
     8521 gcaaaatcgt aagcaggaga ttaatgctca ctggcgggaa ccagacgatc tgctggttaa
     8581 ccgcttcagc gttatcgccc agcatcatgc gggtgaccag aacagcggct cctgcgacga
     8641 acagcactcg cagatattgc ataaacgcca ccaggcgaat atctgcgccg taatcttgcg
     8701 ccattgcgac cattgccgcc gcgccgccag gagaagaacc ccaggccccg gtattcccag
     8761 gcagtgagct atagcgcacc aataaccaac ccacgatggc gctggaaagc agcgtcacca
     8821 gtaaaatcgc aagcacgatc ggccagttga cggccagcgt ggtgagaatc gaaccagtga
     8881 ggttttgtgc aatcatgcag ccaagaatgg cttgggcagc gagaaaagcg gagcggggaa
     8941 gttgcagagt gactccgtgc atactgaaga tgatccccgc gatcatcggg ccaagcagga
     9001 gtgccgccgg gagatgtaaa gcgaggaagc caatagaaag gagaagtgac agaacacata
     9061 acattcccca ctgtagcact ggcatccgtc actccctgtt taacttacct ttggtgtatc
     9121 gatacagcat aaagaatgcg cctcaacgaa aaaatagcgg taatcaagaa aaagacagat
     9181 tttggctaaa gggcgtatta agtaccgttg tctccctctc gtttgtaaaa gagggaagag
     9241 taaacgattg aactgagata agtcttatga cgaggaatta ttgatcagtg cactgaccgg
     9301 gctgtcgaag tgtgatttca tggcttgagt cgccgtttcg acatccctgg cacacacagc
     9361 atcgcagatt ttctggtgta gtgtgatgat gtgattgcca tactctttct gatcgctttg
     9421 ggcgtttaaa ccctcctgga tggattgccg caactgctga tgtaatgcct gcaataacat
     9481 cataaaaagc ggattttttg aggcaacggc aagtaactga tggaagcgga tatcatgggt
     9541 gataaagctt tcaacatcat tgaactgttc gcggattttt gccatttcag cccgtaggtt
     9601 agcaaaatct tcctcttcgc cattacgggc agcagcttcc acaacgccca actccagcca
     9661 gcggcgtact tgaagaattt gctctgttgt cacttgttcg gtcgccaggg caaatccaaa
     9721 caccacgcgc agcacttcat catcaaattt tttgattcgt ggcactttac cgctgtgcgt
     9781 ttccagaatc cccagggcgg caagtgaacg ataggcctca cgcacaatac cccgactgac
     9841 acccaatttc tgaatcagct gaacttcact gggaactggc atccccggtg tcaatttttg
     9901 ttcggcgatc agattaagaa tatgattttt gacctgtgac gataacgtta cgctggtcaa
     9961 taacgcactc tcactcattt ttactcctgt tgtacttttt cctggtcggt gaaatccaga
    10021 ataccgggac tgtatccttc atattttcct ccagaaccct tgattatcaa tagactactg
    10081 cgcctgtgcg cccgccatgc agtgcgaatc aaagacatat tgtgtgaaag agtggcgaag
    10141 aaatagtaaa tacacaactt tttcgtgacg cccttcgcag tattgtgcgc tttttctcct
    10201 taccctgtaa acctgttaaa caggcttaca ggacgaaaat aatgtggaag agtgataacg
    10261 aactttttgc tttgatgaaa acccggctat ttaccgccgt ggttggcgat attctcgaca
    10321 cgctgggcta tatgcaccag tttgtttctc cggcaatcaa gccgctggat gtgaatacgg
    10381 tggtggcagg ccgggcaatg ccggtgctgg agtcggacgt ttacgaaacc aaatcgacgt
    10441 cgggtaagac ggcgattggc gaaaaaccgt ttggcatcat gtttgaagcg ctggatgccc
    10501 tcaaagagaa cgatgtttac atcactacgg gcagctctct gcgctatgcc ttgtggggcg
    10561 ggttgatgtc tacccgtgcc atgcatctga aagcagcagg tgcggtactc gatggttacg
    10621 cccgtgactc tgctgaaatt cttgaattga aatttcccac tttttgtcat ggcacttacg
    10681 cacaggatca aggtccgcgc ggcaaggtca tcgacttcgg cgtacctatt gaatttcacg
    10741 gcgtgcgagt gaatccgggc gatatcgtgt ttggtgatcg tgacggatta ctgattattc
    10801 cccaggttgt tgagaaagaa gttattaccc aggccctgga aaaagtggca actgaaagtg
    10861 aagtgcgcaa agccatcagt gatggcatga gcacggtaca ggcctttgaa accttcggcg
    10921 taatgtaagg agagcaatga atgaaggcat taaccattga gcagggaagt caggcgcgat
    10981 ttgttgatat ccccgaacct gtctgcggta aaggagaagt gctaatcaat gttgagcgtg
    11041 tcggactgtg tggttctgac ctcaatacct ggcggggact aaatccgctg gttaattatc
    11101 cctgtattcc ggggcatgaa attggcggca cggtgctggc aactggcgat atggcagggg
    11161 cgatcaagcc aggtgaccgg gtggtcgttc tgccttgcac tgagtgtggc gtgtgcagct
    11221 cctgtctgag cggtcgtcct aacgcctgta aaaacaacca aactcttggt gtgcaacgtc
    11281 agggggggat ggcgcaacaa ctgacgatgc ctgcacataa agtcattgtc tgtaatgcct
    11341 taagtgttga tgagttagcg ctggtagaac cgctggccgt gggactacat gcggccagac
    11401 gtgcgcaaat taatgtgggt gaatgggtcg cagttatcgg ttgtggggta attggccttg
    11461 gcgctatcgc cgcggcatcc gccatggggg cgcgcgtaat tgctatcgat attgatgatc
    11521 gcaagcggaa cattgccctg gaatgcggtg cgcaacgttt tcttaattcg cagcaatgtg
    11581 acgtggcggc agaactggtg ctgttaaatg acggtcgcgg cccggaagtg gtgattgaag
    11641 cggtgggtaa tcaacaaacc attgaacagg ctacagggtt ggtggcgttt agcggtcgtg
    11701 tggtttatgt cggttacgcc aaacagccag tgagttatca gagtgcgcag ttcctgatga
    11761 aagaactgga tatccgtggt tcgcgcaatg ccaccgtggc ggattttcgt gccgtgctgg
    11821 cattgatgga gtccgggcgt tatccggtca gcaatctggt gagtgctcac tacccgctgg
    11881 aacaggctga tcacgctttt agcgactggg caagtgatcc cggcgcgtat accaaaatcc
    11941 tcattgacct ttaaggagcg acaccatgaa tgctttttct ttacataaca aaaccgcgtt
    12001 gatcaccggt gcagccagtg ggctggggct ggcaatggcg aaatgcatga tagtcagcgg
    12061 ggcgaaggtc attattgctg acctgaacgg cgagctggcg caagcgaccg ccggaacatt
    12121 ggggtcgcag gccagttgga tacaaatgga tgtcggggat accgcagccg cgcaggaaaa
    12181 agttgatgtg cttattagcg aacacggggc tatcgatatc ctggtcaata acgcaggcaa
    12241 tcactgcaaa aaaccgattg aagagatgag cgtcagtgat tttgagtcag tactcgacgt
    12301 tcacgtagtc ggcgcatttg cgttaaccaa agcgctggtg ccgcatatga aacaacgcgg
    12361 taaaggctgt gtgcttttta ctgcatcgat gacctcattt cttgggcagc ctctggttac
    12421 tggctacagc gcagcgaagt cagcctatct cggcatgatt cgcggtatga ccacggagct
    12481 tgccgttcat gggattcgcg tcaatggtat tgcgccaggc tggatcgata caccaatgtt
    12541 gcgtaaggcc attgaaggtg atgacgaacg ccgtaacaaa atcctgggtc gtaccccgat
    12601 gaaaaaattc ggcaagccgg aggatatcgg ttgggctgcg gtttatctgg cttcagacgc
    12661 agcggcgttt gtcagtggtc aggtgctggt ggtggatggc ggcgcattga tcggcttctg
    12721 atgtacggag gaattatgcg caaacgtgcg ttaaagctac atgccaaaga taactgtgta
    12781 gtcgcgctga atgacatcgc cagtggcgat attgtctggt gggatgaggg ggaaatggtg
    12841 gcctgtagtt ccgttaccct ggggcacaaa ctggcctgcg aagcgctgaa caatggtgat
    12901 tcgattctga aatacggcgc cattatcgga cacgccactc gcgatattgc catgggtgaa
    12961 catgtgcatt cccataatct tgccagcaat gccatcgcca tttttcatca tgatgatgcc
    13021 ggctatcagt cagaggaaaa agcataatgg aaggttatgt acggcaaaat ggtcgaccag
    13081 gtattcgtaa tactgtgcag gtaatcttta tggtggagtg tgccagccat gtcgctaaaa
    13141 agattgccga aggatttcct tgtcacgacg tgcagtattt tggtttttca ggttgttatc
    13201 ccagcgatta tggctatcgg ctggtgaaaa atctggcgac tcatagcaac gtaggtggtg
    13261 tactgattat ttcgctgggg tgcgagaatt tcgatcgccg tcggctggag caagaagtac
    13321 gagaaagtgg gcgaccgtgc catacgctgg tcattcagga aaataaaggc acgaccaaca
    13381 ccattactct gggtaagcag ttggtcgctg aaatgctgga acagcttgcg gatacaccgc
    13441 gttgtatcct gaactggagc gacctggtgg ttggtactat ctgcggtggc tccgatggca
    13501 ccagtggtat cacgggtaac ccggcagtag gacgtgcctt tgaccaactg ctggagaaag
    13561 gggctacctg tatttttgaa gaaagcggtg agttgcttgg ttgtgagcag catatgatgt
    13621 cgcgcgcggc tagttcgcag gcacgtgatg ccatcgaagt ggcaatgaca aaagccgaac
    13681 gttattacag tttgatgggg ctggggagtt tttctaacgg taatgctgaa ggcggcttaa
    13741 ccactcagga agaaaaatcc cttggggctt ataccaagtc gggcgatgcg ctgattgctg
    13801 gcgttatttt gccgacgcaa caaacgccct ttcctgggct ttggttgatg gacgtggtgc
    13861 ctgatggtga acctcgtttt gggtatccga atattaacga ttcctcagaa gcactggaac
    13921 ttatcgcctc tggctgtcat atggtgttat ttaccacagg tcgcggttca gttattggtt
    13981 cagtgatcgc tcctattatt aaagtgtgta ataacccgca gacgtgggag catttatctg
    14041 acgatatgga cgttaatgcc ggtcgtgtaa ttagcgaagg cgcaacgctc gacgatattg
    14101 cagacgatat tatgcaggtg attgaacatg tcgccaacgg tcaatattgt gccgcagaac
    14161 gactggaaca tcaggagttt gttttaacgt ataaagcatt tgattacagc aaaagtgcag
    14221 tctgtcgggc agatccccgt taatcgataa agctcttaca ggtaataata atgaaaaaat
    14281 tactgacacc acttttattt cttttagctt tatttagtcc gatgctgaat gccgcaccgg
    14341 taactatcaa aatcgcctat gaaagtaacc ccggcgagcc agcggataca gtagtgaagc
    14401 actgggcaga acttgtgaag aaaaaaagca atggagatat tgttttcgaa ctttacccaa
    14461 gttcacagtt aggttctaaa caagatatta ctgaacaagc gttgatgggg atgaatatta
    14521 tcaccatcag tgatgtagga tttttaactg actacgatgc agacttggga atattgtttg
    14581 ggccttattt aaccgaagat ccgcagaaac tgtttgcgat ttatgccagt gactggttta
    14641 aacaaaaaga acagaaacta cgggaaaaag gtgtgcatat tgtcatgagc aactatctgt
    14701 acggtaccag gcagatcatt gctaaaaaac cgattcgtac acctgaagac ttgaaaggga
    14761 tgaagatccg cgtgccgaat aacgtcatgc agattaaagc tatcgaagcg atgggcgcaa
    14821 ccccaacacc aatgccgctg ggagatgttt atccggcgct gactcagggg atgatcgatg
    14881 gtgtggaaaa cccggtttct gttctctatg ggcaaaagct gcatgagcag gcgaaatatc
    14941 tgtcaatggt gaactatctg accaatacat cattatggtt gggtggcgaa gcgtttttca
    15001 gtaccctgac acctgcgcag ttagatatta ttcaccagac agcgtatgaa gctggtttat
    15061 atagccagaa aattaccact gagcaggatg cgacaatgtt gaaaaccatg caggagcagg
    15121 gcgtggaaat catctatcct gacgtcgccc ctttcaaaga aaaagcctta aaagtctatc
    15181 aacaattccc ggaatggacc cctggtttgt atgacactat tcagcaacag ctgaaataac
    15241 ttcatggcat aaggactggt cggtgtttcg gcaccgaccg ttaagagcag aggtgtttat
    15301 gcgtctttta gggaaaatga ttgaagttgc tgcggtatta acgctcttta tattggtaat
    15361 catgaccatt gctgcggtaa taatgcgtta ctgttttcat atgccattac agtggacaga
    15421 agaggtttcc ggtttattaa tgatttgggt agtcatgttt ggtgcggttg ttgctgaacg
    15481 agataatacc catttatcga ttccattttt agccgatgca cttacttcac gcgggcaacg
    15541 tatattagcg tttttagtct ctctgatgtc tattattttg ctggtggtca tggcctggtc
    15601 agcatggaaa ttagctgccg ggacagcctt taaaaccacg caaatattaa aaatatcctg
    15661 gttttggatc gatattgccg tcacggttgg cgctatcggt gtcgcctgtt ttactgcctt
    15721 acaactgatc aagaaaacgc cacgcaagga aggtgaataa tgacagcatt gagtgaatta
    15781 ctggcgggaa gctggctgga taactggctg atgatcattc cgcttatgtt cgtctgttta
    15841 tttatcaata tgccggtatg gctggcgatg ttttgcggca ttctgaccta cttcctttgc
    15901 tattcaaatg tgccgttaat ggtggcaatt cagcgtttga ttgccacgac gcaaacccct
    15961 tcgctgttag ctattccgtt ttttatctta ttaggcacgt taatgagcta taccggtatt
    16021 gccgcgcgta ttttgcaaat tgccaacgtg ttgattggca gaatgcgcgg tggcctgggt
    16081 ggggctaata ttctggtcag taccatgatg ggcggtgtca gtgcctcaaa tctggcggat
    16141 gccgcaatgt tatcgcgcat gatggtgccg gaaatggaac agaaaggtta ttcccgtggc
    16201 tttgcggcgg cgataacggc aagtggttcg ctgattacac caattattcc accgggtatt
    16261 gcactgatcc tttacggact ggtggcggat gtctccatcg gctccatgtt tatggccggg
    16321 atcctgccag gtctgctgtg ctccgccatg ttgatcgtta ccgtatggtt ggtggctatt
    16381 cgtcgcggtt taaaaccttc cagcgaacac tggccaaaac cgaaagaagt atcagtggcg
    16441 ctggtgcagg cctggccagc actgttctta attgttgcgg ttatcggtgg tattcgggca
    16501 aatatcttta cgccaacgga agccggagcg gtagcagtat tattggtaat gtttattggt
    16561 tttgtaattt atcgcgaact gaaaatcagc catgtattta cagcactctc tgagacggcg
    16621 cgttccacgg catcggtcat gttaatcatt atggcgagtg ccgcgctggg ctggatattt
    16681 tcccttgagc gggcaggtat cacggttgcg gagtttgtaa cttctctgac cgataataaa
    16741 tacgtctttt tattgattat caatctattg ctgctggtgc ttggcatgtt ccttgaaggt
    16801 agtgccattc tgctgatatt agtcccatta cttaaacccg tggtggctca ttttggcatt
    16861 gatccggtgc attttggcat cattatgatc ctcaacttat cgttaggaac gttatcacca
    16921 cctgtcggaa cggtgatgct gctggtatgt aataccgtac aggtttctgt cgggcaattt
    16981 atgcgtgaag cggtgccatt actgatcagt cttattttac ttctgttggt cattacctat
    17041 attccggcaa tatctttgct gctggtgtag aattaaaaac cctgcgtgtc cacgcagggt
    17101 tttgtcggtg tcatttttac ttcttaatat ccgacttaaa atcacgctgc gcgtagccgg
    17161 tatacagctg acgcggacgg gcaatcttca taccgtcact gtgcatttcg ctccagtggg
    17221 cgatccagcc aaccgtacgt gccatagcga agatcacggt gaacatagaa gacggaatac
    17281 ccatcgcttt caggatgata ccagagtaga aatcgacgtt cgggtacagt tttttctcga
    17341 taaagtacgg gtcgttcagc gcgatgtttt ccagctccat agccacttcc agcaggtcat
    17401 ctttggtgcc cagctctttc agcacttcat ggcaggtttc acgcattacg gtggcgcgcg
    17461 ggtcgtagtt tttgtacacg cgatgaccga agcccatcag gcggaaagaa tcatttttgt
    17521 ctttcgcacg acgaacaaat tccggaatgt gtttaacgga gctgatttct tccagcattt
    17581 tcagcgccgc ttcgttagca ccaccgtgcg caggtcccca cagtgaagca atacctgctg
    17641 cgatacaggc aaacgggttc gcacccgaag agccagcggt acgcacggtg gaggtggagg
    17701 cgttctgttc atggtcagcg tgcaggatca gaatacggtc catagcacgt tccagaatcg
    17761 gattaacttc atacggttcg cacggtgtgg agaacatcat attcaggaag ttaccggcgt
    17821 aggagagatc gttgcgcggg taaacaaatg gctgaccaat ggaatacttg taacacatcg
    17881 cggccatggt cggcattttt gacagcaggc ggaacgcggc aatttcacga tgacgaggat
    17941 tgttaacatc cagcgagtcg tgatagaacg ctgccagcgc gccggtaata ccacacatga
    18001 ctgccattgg atgcgagtcg cgacggaaag catggaacag acgggtaatc tgctcgtgga
    18061 tcatggtatg acgggtcacc gtagttttaa attcgtcata ctgttcctga gtcggttttt
    18121 caccattcag caggatgtaa caaacttcca ggtagttaga atcggtcgcc agctgatcga
    18181 tcgggaaacc gcggtgcagc aaaatacctt catcaccatc aataaaagta attttagatt
    18241 cgcaggatgc ggttgaagtg aagcctgggt caaaggtgaa cacacctttt gaaccgagag
    18301 tacggatatc aataacatct tgacccagcg tgcctttcag cacatccagt tcaacagctg
    18361 tgtccccgtt gagggtgagt tttgcttttg tatcagccat ttaaggtctc cttagcgcct
    18421 tattgcgtaa gactgccgga acttaaattt gccttcgcac atcaacctgg ctttacccgt
    18481 ttgttatttg gctcgccgct ctgtgaaaga ggggaaaacc tgggtacaga gctctgggcg
    18541 cttgcaggta aaggatctat tgatgacgaa taaatggcga atcaagtact tagcaatccg
    18601 aattattaaa cttgtctacc actaataact gtcccgaatg aattggtcaa tacttccaca
    18661 ctgttacata agttaatctt aggtgaaata ccgacttcat aacttttacg cattatatgc
    18721 ttttcctagt aatgtt
//
LOCUS       DAHHTG010000086        18459 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.086, whole genome shotgun sequence.
ACCESSION   DAHHTG010000086 DAHHTG010000000
VERSION     DAHHTG010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18459)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18459)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18459
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.086"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            complement(66..629)
                     /gene="fimA"
                     /locus_tag="NBI74_003061"
     CDS             complement(66..629)
                     /gene="fimA"
                     /locus_tag="NBI74_003061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HCD1933353.1"
                     /translation="MKLKHVGIIVVSVLAMSSAAVSAAEGDESVTTTVNGGVIHFKGE
                     VVNAACAIDSESMNQTVELGQVRSSRLAKAGDLSSAVGFNIKLNDCDTNVSSNAAVAF
                     LGTTVTSNDDTLALQSSAAGSAQNVGIQILDRTGEVLVLDGATFSAKTDLIDGTNILP
                     FQARYIALGQSVAGTANADATFKVQYL"
     gene            complement(1574..1810)
                     /locus_tag="NBI74_003062"
                     /pseudo
     CDS             complement(1574..1810)
                     /locus_tag="NBI74_003062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416024.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HipA N-terminal domain-containing protein"
     gene            complement(1810..2076)
                     /gene="hipB"
                     /locus_tag="NBI74_003063"
     CDS             complement(1810..2076)
                     /gene="hipB"
                     /locus_tag="NBI74_003063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HipB"
                     /protein_id="HCD1933354.1"
                     /translation="MMSFQKIYSPTQLANAMKLVRQQNGWTQSELAKKIGIKQATISN
                     FENNPDNTSLTTFFKILQSLELSMTLCDAKNASPEATEQQDLEW"
     gene            2384..3142
                     /gene="tam"
                     /locus_tag="NBI74_003064"
     CDS             2384..3142
                     /gene="tam"
                     /locus_tag="NBI74_003064"
                     /EC_number="2.1.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trans-aconitate 2-methyltransferase"
                     /protein_id="HCD1933355.1"
                     /translation="MSDWNPSLYLHFAAERSRPAVELLARVPLENIEYIADLGCGPGN
                     ITALLHQRWPAARITGIDSSPAMIAEARSALPDCLFVEADIRNWQPEQALDLIFANAS
                     LQWLPDHYELFPHLVSLLNSHGVLAIQMPDNWLEPTHVLMREVAWEQNYPDRGREPLA
                     GVHAYYDILSEAGCEVDIWRTTYYHQMPSHQAIIDWVTATGLRPWLQDLTESEQQHFL
                     TRYHQMLEEQYPLQENGQILLAFPRLFIVARRTE"
     gene            complement(3146..4060)
                     /locus_tag="NBI74_003065"
     CDS             complement(3146..4060)
                     /locus_tag="NBI74_003065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bestrophin family protein"
                     /protein_id="HCD1933356.1"
                     /translation="MIVRPQQHWLRRIFVWHGSVLSKISSRLLLNFLFSIAVIFMLPW
                     YTHLGIKFTLAPFSILGVAIAIFLGFRNNAGYARYVEARKLWGQLMIASRSLLREVKT
                     TLPDSASVREFARLQIVFAHCLRMTLRKQPQAEVLAHYLKTEDLQRVLASNSPANRIL
                     LIMGEWLAVQRRNGQLSDILFISLNDRLNDISAVLAGCERIAYTPIPFAYTLILHRTV
                     YLFCIMLPFALVVDLHYMTPFISVLISYTFISLDCLAEELEDPFGTENNDLPLDAICN
                     AIEIDLLQMNDEAEIPAKILPDRHYQLT"
     gene            complement(4257..5708)
                     /gene="uxaB"
                     /locus_tag="NBI74_003066"
     CDS             complement(4257..5708)
                     /gene="uxaB"
                     /locus_tag="NBI74_003066"
                     /EC_number="1.1.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HCD1933357.1"
                     /translation="MKTLNRRDFPGAQYPERIIQFGEGNFLRAFVDWQIDLLNEHTDL
                     NSGVVVVRPIETSFPPSLSTQDGLYTTIIRGLNEKGEAVSDARLIRSVNREISVYSEY
                     DEFLKLAHNPEMRFVFSNTTEAGISYHAGDKFDDAPAVSYPAKLTRLLFERFSHFNGA
                     LDKGWIIIPCELIDYNGDALRELVLRYAQEWALPEAFIQWLDQANSFCSTLVDRIVTG
                     YPRDEVAKLEEELGYHDGFLDTAEHFYLFVIQGPKSLATELRLDKYPLNVLIVDDIKP
                     YKERKVAILNGAHTALVPVAFQAGLDTVGEAMNDAEICAFVEKAIYEEIIPVLDLPRD
                     ELESFASAVTGRFRNPYIKHQLLSIALNGMTKFRTRILPQLLAGQKANGTLPARLTFA
                     LAALIAFYRGVRNGETYPVQDDAHWLERYQQLWSQYRDRVIGTQELVAIVLAEKDHWE
                     QDLTQVPGLVEQVANDLDAILEKGMREAVRPLC"
     gene            complement(5935..7353)
                     /locus_tag="NBI74_003067"
     CDS             complement(5935..7353)
                     /locus_tag="NBI74_003067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HCD1933358.1"
                     /translation="MHVQPISTFRLFQEGHLLRNSIAIFVLTTLFYFIGAELRLVHEL
                     SLFWPLNGVMAGVFARYVWLNRLHYYAISYVAMLVYDAITTEWGLVSLAINFSNMMFI
                     VTVALLVARDKRLGKNKYEPVSALRLFNYCLLAALLCAIVGAIGSVSIDTLDFWPLLA
                     DWFSEQFSTGVLIVPCMLTLAIPGVLPRFKAEQMMPAIALIVSVIASVVIGGAGSLAF
                     PLPALIWCAVRYTPQVTCLLTFVTGAVEVVLVANSVIDISVGSPFSIPQMFSARLGIA
                     TMAICPIMVSFSVAAINSLMKQVALRADFDFLTQVYSRSGLYEALKSPSLKQTQHLTV
                     MLLDIDYFKSINDNYGHECGDKVLSVFAQHIQKIVGDKGLVARMGGEEFAVAVPSVNP
                     VDGLLMAEKIRKGVELQPFTWQQKTLYLTVSIGVGSGCASYRTLTDDFNKLMVEADTC
                     LYRSKKDGRNRTSTMRYGEEVV"
     gene            complement(7492..7851)
                     /locus_tag="NBI74_003068"
     CDS             complement(7492..7851)
                     /locus_tag="NBI74_003068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4186 domain-containing protein"
                     /protein_id="HCD1933359.1"
                     /translation="MQSLDPLFARLSRSKFRSRFRLGVKERQYCLEKGAPVIEQHAAD
                     FVAKRLAPALPANDGKQTPMRGHPVFIAQHATATCCRGCLAKWHNIPQGEALSEEQQR
                     YIVTVIYHWLVIQMNQP"
     gene            complement(7851..8777)
                     /gene="glsB"
                     /locus_tag="NBI74_003069"
     CDS             complement(7851..8777)
                     /gene="glsB"
                     /locus_tag="NBI74_003069"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase B"
                     /protein_id="HCD1933360.1"
                     /translation="MAVAMDNAILENILRQVRPLIGQGKVADYIPALATVDGSRLGIA
                     ICTVDGQLFQAGDAQERFSIQSISKVLSLVVAMRHYSEEEIWQRVGKDPSGSPFNSLV
                     QLEMEQGIPRNPFINAGALVVCDMLQGRLSAPRQRMLEVVRGLSGVSDISYDTVVARS
                     EFEHSARNAAIAWLMKSFGNFHHDVTTVLQNYFHYCALKMSCVELARTFVFLANQGKA
                     IHIDEPVVTPMQARQINALMATSGMYQNAGEFAWRVGLPAKSGVGGGIVAIVPHEMAI
                     AVWSPELDDAGNSLAGIAVLEQLTKQLGRSVY"
     gene            complement(8841..10229)
                     /gene="sad"
                     /locus_tag="NBI74_003070"
     CDS             complement(8841..10229)
                     /gene="sad"
                     /locus_tag="NBI74_003070"
                     /EC_number="1.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate-semialdehyde dehydrogenase"
                     /protein_id="HCD1933361.1"
                     /translation="MTITPATHAISINPATGEQLSVLPWAGVDDIENALQLAAAGFRD
                     WRETNIDYRAEKLRDIGKALRARSEEMAQMITREMGKPINQARAEVAKSANLCDWYAE
                     HGPAMLKAEPTLVENQQAVIEYRPLGTILAIMPWNFPLWQVMRGAVPIILAGNGYLLK
                     HAPNVMGCAQLVAQVFKDAGIPQGVYGWLNADNDGVSQMIKDSRIAAVTVTGSVRAGA
                     AIGAQAGAALKKCVLELGGSDPFIVLNDADLELAVKAAVAGRYQNTGQVCAAAKRFII
                     EEGIASAFTERFVAAAAALKMGDPRDEDNALGPMARFDLRDELHHQVEKTLAQGARLL
                     LGGEKMAGAGNYYPPTVLANVTPEMTAFREEMFGPVAAITIAKDAEHALELANDSEFG
                     LSATIFTTDETQARQMAARLECGGVFINGYCASDARVAFGGVKKSGFGRELSHFGLHE
                     FCNIQTVWKDRV"
     gene            10330..11211
                     /locus_tag="NBI74_003071"
     CDS             10330..11211
                     /locus_tag="NBI74_003071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCD1933362.1"
                     /translation="MDLTQLEMFNAVAEAGSITQAAAKVHRVPSNLTTRLRQLETELG
                     VDLFIRENQRLRLSPAGHNFLRYSQQILALVDEARSVVAGDEPQGLFSLGSLESTAAV
                     RIPATLAEFNRRYPKIQFSLSTGPSGTMLEGVLEGKLNAAFIDGPINHTAIDGIPVYR
                     EELMIVTPQGHAPVTRASQVNGSNIYAFRANCSYRRHFESWFHADGAAPGTIHEMESY
                     HGMLACVVAGAGIALIPRSMLESMPGHHQVESWPLAEQWRWLTTWLVWRRGAKTRPLE
                     AFIQLLDAPDSAKQGYQ"
     gene            11289..11372
                     /gene="yneK"
                     /locus_tag="NBI74_003072"
                     /pseudo
     CDS             11289..11372
                     /gene="yneK"
                     /locus_tag="NBI74_003072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416044.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="putative protein YneK"
     gene            11550..12740
                     /gene="ydeA"
                     /locus_tag="NBI74_003073"
     CDS             11550..12740
                     /gene="ydeA"
                     /locus_tag="NBI74_003073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose MFS transporter"
                     /protein_id="HCD1933363.1"
                     /translation="MTTNTVSRKVAWLRVVTLAVAAFIFNTTEFVPVGLLSDIAHSFH
                     MQTAQVGIMLTIYAWVVALMSLPFMLMTSQVERRKLLICLFVVFIASHVLSFLSWSFT
                     VLVISRIGVAFAHAIFWSITASLAIRMAPAGKRAQALSLIATGTALAMVLGLPLGRIV
                     GQYFGWRMTFFAIGIGALLTLLCLIKLLPLLPSEHSGSLKSLPLLFRRPALMSIYLLT
                     VVVVTAHYTAYSYIEPFVQNIAGFSANFATALLLLLGGAGIIGSVIFGKLGNQYASAL
                     VSTAIALLLVCLALLLPAANSEIHLGVLSIFWGIAMMIIGLGMQVKVLALAPDATDVA
                     MALFSGIFNIGIGAGALVGNQVSLHWSMSMIGYVGAVPAFAALIWSIIIFRRWPVTLE
                     EQTQ"
     gene            complement(12765..13430)
                     /gene="marC"
                     /locus_tag="NBI74_003074"
     CDS             complement(12765..13430)
                     /gene="marC"
                     /locus_tag="NBI74_003074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter MarC"
                     /protein_id="HCD1933364.1"
                     /translation="MLDLFKAIGLGLVVLLPLANPLTTVALFLGLAGNMNSAERNRQS
                     LMASVYVFAIMMVAYYAGQLVMDTFGISIPGLRIAGGLIVAFIGFRMLFPQQKAIDSP
                     EVKSKSEELEDEPSANIAFVPLAMPSTAGPGTIAMIISSASTVRQSSTFADWVLMVAP
                     PLIFFLVAVILWGSLRSSGAIMRLVGKGGIEAISRLMGFLLVCMGVQFIINGILEIIK
                     TYH"
     gene            13642..14076
                     /gene="marR"
                     /locus_tag="NBI74_003075"
     CDS             13642..14076
                     /gene="marR"
                     /locus_tag="NBI74_003075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance transcriptional
                     regulator MarR"
                     /protein_id="HCD1933365.1"
                     /translation="MKSTSDLFNEIIPLGRLIHMVNQKKDRLLNEYLSPLDITAAQFK
                     VLCSIRCAACITPVELKKVLSVDLGALTRMLDRLVCKGWVERLPNPNDKRGVLVKLTT
                     SGAAICEQCHQLVGQDLHQELTKNLTADEVATLEHLLKKVLP"
     gene            14097..14480
                     /gene="marA"
                     /locus_tag="NBI74_003076"
     CDS             14097..14480
                     /gene="marA"
                     /locus_tag="NBI74_003076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     MarA"
                     /protein_id="HCD1933366.1"
                     /translation="MSRRNTDAITIHSILDWIEDNLESPLSLEKVSERSGYSKWHLQR
                     MFKKETGHSLGQYIRSRKMTEIAQKLKESNEPILYLAERYGFESQQTLTRTFKNYFDV
                     PPHKYRMTNMQGESRFLHPLNHYNN"
     gene            14512..14730
                     /gene="marB"
                     /locus_tag="NBI74_003077"
     CDS             14512..14730
                     /gene="marB"
                     /locus_tag="NBI74_003077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance protein MarB"
                     /protein_id="HCD1933367.1"
                     /translation="MKPLLSAIATALILFSAQGVAEQTTQPVVTSCGNVVVVPPSQEQ
                     PPFDLNHMGTGSDKSDALGVPYYNQHAM"
     gene            complement(14761..15660)
                     /gene="eamA"
                     /locus_tag="NBI74_003078"
     CDS             complement(14761..15660)
                     /gene="eamA"
                     /locus_tag="NBI74_003078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416050.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylserine/cysteine exporter"
                     /protein_id="HCD1933368.1"
                     /translation="MSRKDGVLALLVVVVWGLNFVVIKVGLHNMPPLMLAGLRFMLVA
                     FPAIFFVARPKVPLNLLLGYGLTISFAQFAFLFCAINFGMPAGLASLVLQAQAFFTIV
                     LGAFTFGERLHGKQLTGIALAIFGVLVLIEDSLNGQHVAMLGFMLTLAAAFSWACGNI
                     FNKKIMSHATRPAVMSLVIWSALIPIIPFFAASLILDGSATMIHSLVTIDMTTILSLM
                     YLAFVATIVGYGIWGTLLGRYETWRVAPLSLLVPVVGLASAALLLDERLTGLQFFGAV
                     LIMTGLYINVFGLRWRKAVKVGS"
     gene            15855..17042
                     /gene="ydeE"
                     /locus_tag="NBI74_003079"
     CDS             15855..17042
                     /gene="ydeE"
                     /locus_tag="NBI74_003079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux MFS transporter YdeE"
                     /protein_id="HCD1933369.1"
                     /translation="MNLSLRRSTSALLASSLLLTIGRGATLPFMTIYLSRQYSLSVDL
                     IGYAMTIALTIGVVFSLGFGILADKFDKKRYMLLAITAFASGFIAIPLVNNVTLVVLF
                     FALINCAYSVFATVLKAWFADNLSSTSKTKIFSINYTMLNIGWTIGPPLGTLLVMQSI
                     NLPFWLAAICSAFPMLFIQIWVKRSEKIIATETGSVWSPKVLLQDKALLWFTCSGFLA
                     SFVSGAFASCISQYVMVIADGDFAEKVVAVVLPVNAAMVVTLQYSVGRRLNPANIRAL
                     MTAGTFCFVIGLVGFIFSGNSLLMWGMSAAVFTVGEIIYAPGEYMLIDHIAPPGMKAS
                     YFSAQSLGWLGAAINPLVSGIVLTSLPPFSLFIILALVIVVAWVLMLKGIRARPWGQP
                     ALC"
     gene            17169..17264
                     /gene="mgtS"
                     /locus_tag="NBI74_003080"
     CDS             17169..17264
                     /gene="mgtS"
                     /locus_tag="NBI74_003080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein MgtS"
                     /protein_id="HCD1933370.1"
                     /translation="MLGNMNVFMAVLGIILFSGFLAAYFSHKWDD"
     gene            complement(17483..18373)
                     /gene="dgcZ"
                     /locus_tag="NBI74_003081"
     CDS             complement(17483..18373)
                     /gene="dgcZ"
                     /locus_tag="NBI74_003081"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcZ"
                     /protein_id="HCD1933371.1"
                     /translation="MIKKTTEIDAILLNLNKAIDAHYQWLVSMFHSVVARDASKPEIT
                     DNHSYGLCQFGRWIDHLGPLDNDELPYVRLMDSAHQHMHNCGRELMLAIVENHWQDAH
                     FDAFQEGLLSFTAALTDYKIYLLTVRSNMDVLTGLPGRRVLDESFDHQLRNAEPLNLY
                     LMLLDIDRFKLVNDTYGHLIGDVVLRTLATYLASWTRDYETVYRYGGEEFIIIVKATN
                     DEEACRAGVRICQLVDNHAITHSEGHINITVTAGVSRAFPEEPLDVVIGRADRAMYEG
                     KQTGRNRCMFIDEQNVINRV"
BASE COUNT         4886 a         4608 c         4249 g         4716 t
ORIGIN      
        1 tgcgtagcca tgccacacac accctgtttc aatttcagat gacgcatagt caccttaaga
       61 gtgaattata aatattgaac tttgaaggtc gcatcagcat ttgcagtacc agctactgac
      121 tgcccgagag caatataacg agcctggaac ggcagtatat tcgtgccatc aatcaagtca
      181 gttttagcgc taaaagtagc accatcaagt actaatacct cccctgtacg gtccaaaatt
      241 tggataccga cattttgggc agagcctgcc gctgaactct gcagcgctaa tgtatcgtca
      301 ttactggtga cagtagtacc caggaatgca acagctgcat tactggaaac attggtatca
      361 caatcattca gcttgatatt gaagccaacg gcggagctga ggtcacccgc tttagccagg
      421 cgagaagaac gaacctgacc cagctcaacc gtttggttca ttgattctga atcaatcgca
      481 caagcggcat ttaccacttc acctttaaaa tgaattacac cgccattaac agtggtcgtt
      541 actgattcat caccctcggc tgcgcttacc gcagcagacg acatcgccaa cacagaaacg
      601 acaataatac cgacatgttt gagtttcata attctccaaa atttatattt aagtagctga
      661 ataaagagag ttaacaatta agttattctg ttattgctta taaaacaaga atcattcttg
      721 attggatgtt gccctgagaa tatagtttcc atggttatta catttcacga agaatgataa
      781 tatctttaaa ttacaaggac acctattcac aacaagttta ttgctgcact tgttgtagag
      841 agtatttgta aaaacatttt atgtcgctaa ataatatgac gatttcatta tgcatgttat
      901 ttataaacag cgactaaaca gaatattatt gcgaacaagg gaagtgtcag gaaaggtcaa
      961 gaggtactaa tagtttatat ggctataaac attagctatg gttaatgttt gcctggtgaa
     1021 actaactgaa tcccatgaat aatgcagtaa ttagtagtgc ttcatatctg atacctgttt
     1081 taaacgatat atttggaagt tgacgaatat cggaaatagc ttcctccatc agctgtagtc
     1141 actgttctct gtatttcaca ttgcatatca acacgatatg ctgagattag ctaacttgag
     1201 attaaagata attacacggc gctatgaatt atattcagaa gcgtgaggga tatagtgcca
     1261 tcaaagcgct ggctgctgta aacaacgcaa ttatccagct catggtctat ggtgtgccat
     1321 cactgaacag aggttgattt ttctaccgaa acgggaggga agtcagaaat cacaacgtat
     1381 tgatttatct gtgtgtggat gtgaatggtg aagtaaagga ccttcacagt caatttaaca
     1441 taatatacat tatgcgcacc aatataaacc aagggcgaat ccagtcgttg gcggttcatg
     1501 attgttctgc tcattaacaa cgaccaaaac cccatatctt acttaatacc gtattctcgg
     1561 cttaaccgat cgtataacgt ttaacgatcc ggtcacgtac aatcgggcta tcgggtaaca
     1621 ggttatcgaa gaagttaaat acggcatcag aggtgatatt ccccctctgc aatggcagcg
     1681 aaagtgacaa cggtctggca taacggctgg ctaaccactc agctgcatac ttaaaggtgt
     1741 gcgcgccgtt ggctaacttc gttaactcgc ctacccgctg gttgttcatc caagtgacta
     1801 gtttaggcat taccactcca gatcttgctg ttctgttgct tctggcgagg catttttcgc
     1861 gtcgcatagc gtcattgaga gttcaagcga ctgtaaaatc ttaaaaaatg tcgtgagcga
     1921 ggtattgtca ggattgtttt cgaaattgga aattgtcgcc tgcttaatgc cgattttttt
     1981 cgccaactcg ctctgcgtcc agccgttttg ctggcgaacc agtttcattg cattcgccaa
     2041 ttgcgttggg ctatagatct tctgaaagct catcatgtca tacgtccaca gcaaatttat
     2101 ccgcttaagg ggatattatc agttttatcc tttagtgagg ataaagtcaa tctggaaaca
     2161 catcttatcc gctctacggg ataaacgctg cgatatccgc gatcgcggat aaaaaggagg
     2221 atttaaaaaa gcacatccta atgcggtggc ttctttgact accggaatct gtaatcttaa
     2281 tgaccgggcc gcgtaaaaaa cgtctgttca atggggtgat gccgtgaggc gaatttatca
     2341 gttttatcta caattggggt aacgcgctga cgggagtaaa aaaatgtctg actggaaccc
     2401 ctctttatat ctgcactttg ccgctgaacg atcgcgtccg gcggtggagc tgcttgccag
     2461 ggtgccgctg gagaatatcg aatacattgc cgatcttggc tgtggcccag gtaacatcac
     2521 cgcccttcta caccaacgtt ggcctgctgc caggataacg ggcatcgact catctccggc
     2581 gatgattgct gaagcgcgca gtgctttgcc agactgcctg tttgtggaag cagatatccg
     2641 caactggcaa ccggaacagg cactcgatct gatttttgct aatgcctcac tgcaatggct
     2701 gcccgaccac tacgaattgt ttcctcattt ggtttcgtta cttaattcgc atggtgtgtt
     2761 ggcaatacag atgccagata actggctaga gccaacccat gtgctcatgc gcgaagtcgc
     2821 ctgggaacaa aattatccgg atcgcgggcg tgagccgctg gctggcgttc atgcttacta
     2881 cgatattctg agtgaagccg gatgtgaggt cgatatctgg cgaaccactt actatcacca
     2941 gatgccgtcg caccaggcga ttatcgattg ggtgaccgcc actggattac gtccgtggtt
     3001 acaggatctg accgagagcg aacagcagca ttttcttacg cgctaccatc agatgctgga
     3061 agagcaatat cccctccagg agaacggaca gatattgctg gcatttccgc gtctgtttat
     3121 tgttgcccgc cgtacggagt aattatcacg tcagctggta atggcgatcg ggaagaattt
     3181 tcgctggaat ttctgcttca tcattcatct gtaacaggtc aatttcaata gcgttgcaga
     3241 tggcatccag cggtaaatca ttgttttcag taccgaaggg atcttccagt tcttccgcca
     3301 gacaatccag cgaaataaaa gtgtaggaaa tcagcacaga gataaaaggc gtcatgtaat
     3361 gcaggtccac gaccagcgca aacggcaaca tgatacaaaa cagatacacg gtgcgatgca
     3421 aaatcagggt gtaggcaaag ggaattggcg tataggcaat gcgctcgcat cctgccagga
     3481 ccgctgaaat atcattaagc cgatcgttga ggctaataaa caggatatct gaaagttgtc
     3541 cattgcgtcg ctgaaccgcc aaccactccc ccattattaa caagatgcgg ttagctggag
     3601 agttagaagc cagtacacgc tgaagatctt cagtcttgag ataatgagcc agcacttctg
     3661 cctgtggctg tttgcgtaat gtcatgcgta aacagtgggc gaagacgatt tgcagccggg
     3721 caaactccct tacacttgcc gaatccggta atgtcgtttt tacctcgcgc agtaacgacc
     3781 gcgaggcaat catcaactgc ccccaaagtt ttcgcgcttc aacgtaacgg gcgtaaccgg
     3841 cattattacg aaaaccaaga aaaatggcga tggcgacacc gagaatgctg aacggtgcga
     3901 gggtgaattt aatgcccaaa tgcgtgtacc agggcagcat gaaaataaca gcgatcgaaa
     3961 agagaaaatt gagtagtaag cgcgaggata tcttggataa tactgaaccg tgccagacaa
     4021 aaatacggcg cagccagtgt tgttgtggac gaacaatcat ggttatcttc aggcgtggaa
     4081 aagtcgccct attaaacgtg attatgatca cattctcaag atactactta caaattacct
     4141 acaagattac aacattaaca ggtgctgcat gtggcacctg ttaatgataa aggcatatag
     4201 aatgttgtaa ctaatatagc ggaataagaa acccggtcga aaccgggttc agagtattag
     4261 cacaacgggc gtacagcttc gcgcatccct ttttcgagaa tcgcatccag gtcattagca
     4321 acctgctcga ccagaccagg cacttgcgtc aggtcctgct cccagtggtc tttttctgcc
     4381 aacacaatcg ctaccagttc ctgggtcccg ataacacggt cacgatactg gctccagagt
     4441 tgctgataac gttccagcca gtgtgcatcg tcctgtaccg gatatgtttc tccattacgc
     4501 acaccgcgat agaacgcaat caatgccgct aatgcgaaag taaggcgcgc cggaagtgtg
     4561 ccgtttgcct tctgccctgc cagcagctgc ggcaggatgc gtgtgcggaa cttggtcata
     4621 ccgttgagcg cgatagacag cagttgatgc ttgatgtacg ggttacggaa acgtccggtg
     4681 actgcactgg cgaaagactc cagctcatcg cgtggcaaat ccagtaccgg aataatttct
     4741 tcgtagatgg ctttttcaac gaatgcgcaa atttcagcat cgttcatcgc ttcacctacg
     4801 gtatccagcc ctgcctggaa cgccaccggc accagcgcgg tgtgcgcacc gttgaggatt
     4861 gccactttgc gttctttata cggtttaata tcgtcaacga tcagcacgtt aagcggatat
     4921 ttgtccagac gaagttcagt cgctaaggat ttcggcccct gaatcacaaa caggtaaaag
     4981 tgttcagcgg tgtcgaggaa accatcgtgg tagcccagtt cttcttccag tttagccact
     5041 tcatcacgcg gataaccggt aacgatacgg tccaccagcg tagaacagaa gctgttagcc
     5101 tgatccagcc attgaataaa tgcttcaggc aatgcccact cttgcgcata gcgcagcacc
     5161 agttcacgca acgcgtcgcc attatagtca atcaactcac acgggatgat gatccaacct
     5221 ttatccagcg caccgttgaa atggctaaaa cgttcgaaca gcagacgggt cagttttgcc
     5281 ggataactta ccgctggcgc gtcatcgaat ttatcgcccg catggtagct gatacccgct
     5341 tcggtggtgt tagagaaaac aaagcgcatt tccgggttgt gagccagttt caggaattca
     5401 tcgtattcac tatagacgct gatttcacga ttaaccgagc gaatcagacg cgcgtcgctg
     5461 accgcttcac ccttctcatt caggccacgg ataatggtgg tatacagacc atcctgcgtg
     5521 ctcagtgacg gcgggaatga agtttcaatc ggacgaacaa cgaccacgcc agaattcaga
     5581 tcggtgtgct cattcaggag atcgatttgc cagtcaacaa aggcgcgcag gaagttacct
     5641 tcaccaaact gaatgatacg ttctggatac tgtgcaccgg gaaaatcgcg acgatttagt
     5701 gttttcacaa tgggttccct tctgattagt catacaacct gtttgaattg gtacgacagg
     5761 ttagcaaact ttaatacgtc gaacccctgt tttgatcaac tcctgatgat taatgagcag
     5821 ttttatgaga aaagtgtggc gcggatcatg gtttaatcga ggaaacaggc cttttcttgg
     5881 atcataaagt ggtagaacac attgcattta attcgctctg agtaaataaa gatgtcagac
     5941 aacttcctca ccgtaacgca tagtgctggt acggttgcgc ccatctttct tcgagcgata
     6001 cagacacgta tcggcttcga ccatcaattt attaaagtca tcggtcagcg ttcggtacga
     6061 tgcgcaacca ctaccgacgc caatacttac cgtgagataa agcgtttttt gttgccaggt
     6121 gaacggttgc agttcaacgc ctttacggat tttttccgcc attagcaggc catcgacagg
     6181 attcacagac ggcactgcaa cagcaaattc ttcgccgccc attcgcgcca ccagcccctt
     6241 atcaccgaca atcttctgaa tatgctgggc aaacacgctt aacactttat cgccacattc
     6301 atgtccatag ttatcgttaa tgcttttgaa atagtcgata tcaagcagca tgaccgtcag
     6361 atgttgtgtc tgtttcagcg atggactttt cagcgcctca taaagaccgg accgtgagta
     6421 aacctgggtc agaaaatcaa aatcggctcg cagcgcaact tgcttcatta gcgaattgat
     6481 cgctgccacg ctaaaggaaa ccataattgg gcatatcgcc atcgtggcaa taccgagacg
     6541 tgcggagaac atttgcggaa tggagaacgg cgaaccgacc gagatatcaa tcaccgaatt
     6601 tgccaccagt acgacttcca cagcgccggt gacaaaggtc aacagacatg ttacttgcgg
     6661 cgtatagcgc actgcacacc agattaatgc agggagcgga aacgccagac tccccgctcc
     6721 gccaatgact accgaggcaa taaccgacac aataagcgcg atagcaggca tcatctgctc
     6781 tgctttaaag cgcggcagca ctccaggaat tgccaacgtc agcatacaag gcacgatcaa
     6841 cacgcccgtt gagaattgct cactaaacca gtcggcaagc aaaggccaga aatccagagt
     6901 atcaatactg accgaaccaa tcgccccgac aatagcgcat aataatgcgg caagcagaca
     6961 gtaattaaat aaccgtaagg cacttactgg ctcatactta ttttttccca gacgcttatc
     7021 ccgcgcgacc agtaaggcga cggtaacaat aaacatcata ttggagaaat tgatagccag
     7081 tgaaaccagc ccccattcgg tggttatggc atcataaacc agcatcgcca cataactgat
     7141 cgcatagtaa tgcagtcgat taagccagac atagcgggca aacacccccg ccattacgcc
     7201 attcagcggc cagaaaagag aaagttcgtg aaccagccgt aactctgcac caataaaata
     7261 gaacagcgtg gttagcacaa aaatagcgat gctattacgt agcagatgac cttcctggaa
     7321 caaacggaaa gttgaaatgg gctgtacatg cattaattat ctgtccatac aattgcgggt
     7381 ggcaattatc tcaacgcaat aagtaaattc ttagaaagga tagcatgttc gctgacaacg
     7441 gcggcaacta aagataccta atgaatgcca ttttatcgat ttccgtaatt atcatggctg
     7501 gttcatctgt ataaccagcc agtgatagat taccgtcaca atataacgtt gttgctcctc
     7561 acttaacgct tccccctgag gaatgttatg ccacttagcc agacaaccgc gacaacaggt
     7621 cgcggtggcg tgttgagcga taaaaaccgg atgcccgcgc atgggggttt gcttaccgtc
     7681 gttagccggt aatgccggag caagacgttt agcgacaaaa tctgccgcat gttgttcaat
     7741 gactggcgcg cctttctcca gacaatactg acgctctttc acacccaaac gaaagcgaga
     7801 gcgaaatttt gaacgggata aacgcgcgaa gagtggatcg agagactgca ttaataaact
     7861 gaacgcccta attgtttcgt caattgttca aggacggcaa tgcccgcaag cgagttaccg
     7921 gcatcatcca gttccggact ccagacagcg atggccattt catgcggaac aatcgccaca
     7981 ataccgccac caacgccaga ttttgccggt agtcccaccc gccaggcaaa ctcccccgcg
     8041 ttctggtaca taccgctggt cgccatcagc gcgttaattt gccgcgcctg cattggcgtc
     8101 accacaggtt catcaatatg aatagctttc ccctgattcg ccagaaagac aaacgtccgg
     8161 gccagctcta cacagctcat tttcagagcg cagtaatgaa agtagttttg cagaacggtt
     8221 gtcacgtcat gatggaaatt gccaaacgac ttcatcagcc aggcgatagc cgcatttcgc
     8281 gcggaatgtt caaattcgga acgcgctacc accgtatcgt aggaaatatc agacacacca
     8341 cttaagccgc gcacgacttc cagcatacgt tgccgaggtg cgcttaatcg cccttgcaac
     8401 atatcgcaga ccaccagcgc accggcatta ataaacggat tacgcggaat accctgctcc
     8461 atttccagtt gcactaaaga attgaatggt gatccagacg gatctttgcc gacgcgttgc
     8521 cagatttcct cttcggagta atgacgcatg gcgacaacga gactcagcac tttggaaata
     8581 gactgaatgg aaaaacgttc ttgcgcgtct ccggcctgaa aaagctgtcc gtcaacggta
     8641 cagatagcaa tccccaatcg ggaaccgtct accgtagcca gcgccggaat ataatccgcg
     8701 actttaccct gaccaatgag cggccgcact tgccgcaaga tgttttctaa aattgcatta
     8761 tccatggcga ctgccacttt ctactcctgg accgcaggtc tgaaaagacc tgcgagtata
     8821 tcagagctga atatatagcg tcagacccgg tctttccaca ccgtctggat attacagaat
     8881 tcgtgtaagc cgaaatggga aagctcacga ccaaagccac tcttcttcac gccaccaaag
     8941 gcgactcgcg catcgctggc acaataacca ttgataaaca ccccaccgca ttccagacgt
     9001 gccgccatct gtctggcctg tgtttcgtcg gtggtgaaaa tggtcgctga aaggccgaac
     9061 tcactatcat tagccagttc cagcgcatgt tctgcatctt tcgcaatggt gattgccgca
     9121 acggggccaa acatttcttc ccgaaacgcg gtcatttctg gggtaacatt cgccagaacc
     9181 gttggcggat agtagttacc tgccccagcc atcttttccc cgcccagtaa caaacgcgca
     9241 ccctgcgcca gtgttttttc cacctgatga tgcagctcat cacgtaaatc aaaacgagcc
     9301 attggtccga gagcgttgtc ttcatcacgg ggatcgccca ttttcaaggc tgccgcagct
     9361 gccacaaaac gttcggtaaa tgccgaagca attccctctt cgatgataaa gcgttttgcc
     9421 gctgcacaaa cctgtccggt attctgataa cgtccggcta ccgccgcttt caccgccagt
     9481 tccagatcgg catcgttaag cacaataaac ggatccgaac cacccagttc cagtacgcat
     9541 tttttcagtg ccgctccagc ctgtgcgcca atagccgctc cagcacgaac acttccggtc
     9601 actgtgacag cagcaatgcg cgaatcttta atcatctgac tgacaccgtc gttgtcggca
     9661 ttcagccagc catatacgcc ttgtgggata cccgcatctt taaacacctg ggcaacgagc
     9721 tgtgcacagc ccatcacatt cggcgcatgt ttaagtaagt agccgttacc agcaagaatg
     9781 atgggaacag cgccacgcat cacctgccat aacggaaaat tccacggcat aatcgccaga
     9841 atcgtcccca acggtcgata ctcaataacc gcctgctgat tttccaccag cgtaggttct
     9901 gccttcagca ttgccggacc atgttctgca taccagtcac acaaattcgc cgatttcgcc
     9961 acttcagcgc gcgcctggtt gattggtttg cccatttcac gggtgatcat ttgcgccatt
    10021 tcttcgctac gggcgcgcag agccttaccg atatcacgca gtttttcagc acgataatct
    10081 atatttgtct cgcgccagtc gcgaaagcct gctgccgcca gctgaagtgc gttttcgata
    10141 tcgtcaacac cagcccacgg cagcacagaa agttgttcac cggtggcagg atttatcgaa
    10201 attgcatgag ttgccggagt aatggtcatc ggggtatctc ctttatgagt catggtatga
    10261 agatacgcag atttactctt gctttaaaat gaataatatt aagccactta ttcacgaatc
    10321 gagaatgcca tggatctgac ccaactggag atgttcaacg cggttgccga agctggcagc
    10381 attacccagg ctgcagcaaa agtgcatcgc gtgccgtcga atctcactac ccgtctacgt
    10441 cagctggaaa cagaactggg ggttgatctg tttattcgcg agaatcagcg tttacgtctc
    10501 tcgcctgccg ggcataactt tttacgctat agccaacaaa ttctcgcatt agtggatgaa
    10561 gcgcggagcg ttgtcgctgg cgatgagccg caaggtttat tttctctggg atcgctggaa
    10621 agcaccgctg cagtgcgcat tccggccacg ctggcggaat ttaaccgtcg ttatcccaaa
    10681 attcagtttt cactttccac cggcccttcc ggcactatgc tggaaggtgt gctggaggga
    10741 aaactgaatg cggcgtttat tgatggaccc attaaccata ctgccatcga cgggataccg
    10801 gtataccgcg aggaactgat gatcgtcacg ccacaaggac atgcgccagt aacccgtgcc
    10861 agtcaggtga atggcagtaa catttatgcc ttccgcgcca attgttcgta tcgtcgccac
    10921 ttcgaaagct ggtttcatgc tgacggtgcc gctccgggaa ctattcacga gatggagtct
    10981 tatcacggga tgttggcctg tgtggtcgca ggagcaggca ttgcgcttat tccacgctct
    11041 atgctggaaa gtatgccggg gcatcaccag gttgaatcgt ggccgttagc tgagcaatgg
    11101 cgttggttaa caacctggct ggtctggcgt cgtggtgcga aaacgcgtcc gcttgaggca
    11161 tttattcaac tgctggatgc gcctgactcg gcaaaacagg gttaccaatg agctattttt
    11221 gatagttcat gcggctaata tgctctatat agtgatgttc caatgactta tgactatatg
    11281 gggcaaatat ggttacgcca gtaagcatca gcaattacat atctcttcct gatgattttc
    11341 ccgttcgtaa tattgcacct caagtaaaag aagaacaggc ccccttcctg atcgtcatta
    11401 aaatgtgatg ttaacaactc tctgatgcgt gacagaacaa ccgtcagaga gatggaaatg
    11461 cgatccagat cacaaatgca ttgtattcac atcattaacc gttttaagat catttcatca
    11521 ctttttcgca actcacccga taatctgtta tgacaacaaa cactgtttcc cgcaaagtag
    11581 cgtggctacg ggtcgttacg ctggcagtcg ccgcctttat cttcaatacc accgaatttg
    11641 tccctgttgg cctgctctct gacattgcgc atagttttca catgcaaacc gctcaggtcg
    11701 gcatcatgtt gacaatttat gcatgggtag tggcgctaat gtcattgcct tttatgttaa
    11761 tgaccagcca ggttgaacgc cgcaaattac tgatctgcct gtttgtggtg tttattgcca
    11821 gccacgtact gtcgttttta tcgtggagtt ttaccgttct ggtgatcagt cgcattggtg
    11881 tggcttttgc acatgcgatt ttctggtcga ttacggcgtc tctggcgatc cgtatggctc
    11941 cggccgggaa gcgagcacag gcattgagtt taattgccac cggtacagca ctggcaatgg
    12001 tattaggttt acctctcggg cgcattgtgg ggcagtattt tggctggcga atgaccttct
    12061 tcgcgattgg tatcggggcg cttcttaccc ttttgtgcct gattaagtta cttcccttgc
    12121 tgcccagtga gcattccggt tcattgaaaa gcctcccgct attgttccgc cgcccggcat
    12181 tgatgagcat ttatttgtta actgtggtgg ttgtcaccgc ccattacacg gcatacagct
    12241 atatcgagcc ttttgtacaa aacattgcgg gattcagcgc caactttgcc acggcattac
    12301 tgttattact cggtggtgcg ggcattattg gcagcgtgat tttcggtaaa ctgggtaatc
    12361 agtatgcgtc tgcgttggtg agtacagcga ttgcgctatt gctggtgtgc ctggcactgc
    12421 tgctacctgc ggcgaacagt gaaatacacc tcggggtgct gagtattttc tgggggatcg
    12481 cgatgatgat catcgggctt ggtatgcagg ttaaagtgct ggcgctggca ccagatgcca
    12541 ccgacgtcgc gatggcgcta ttctccggga tatttaatat tggaatcggc gcgggtgcgt
    12601 tggtaggtaa tcaggtgagt ctgcactggt caatgtcgat gattggttat gtgggcgcgg
    12661 tgcctgcttt tgctgcgtta atatggtcaa tcatcatttt tcgccgctgg ccagtgacac
    12721 tcgaagaaca gacgcaatag ttgaaaggcc cattcgggcc ttttttaatg atacgtttta
    12781 atgatttcca ggatgccgtt aataataaac tgcacgccca tacataccag caggaatccc
    12841 atcagacggg agatcgcttc aataccaccc ttgcccacca gccgcataat tgcgccagag
    12901 ctgcgtagac ttccccacaa aataaccgcc accaggaaaa agatcagcgg cggcgcaacc
    12961 atcagtaccc aatcagcgaa ggttgaactc tgacgcactg tggaggccga gctaataatc
    13021 atcgcaatgg ttcccggacc ggcggtactt ggcattgcca gtggtacaaa agcaatattg
    13081 gcactgggtt catcttccag ctcttccgac ttgcttttca cttccggtga atcaatcgct
    13141 ttctgttgcg gaaagagcat ccgaaaaccg ataaacgcga cgatcaaacc gcctgcaatt
    13201 cgcagaccgg gaatcgaaat gccaaatgta tccatcacca gttgcccggc gtaatacgcc
    13261 accatcatga tggcaaatac gtacaccgag gccatcaacg actgacgatt acgttcggca
    13321 ctgttcatgt tgcctgccag gccaagaaat aacgcgacag ttgttaatgg gttagctaac
    13381 ggcagtaaca ccaccagccc caggcctatt gctttaaaca aatctaacat tggtggttgt
    13441 tatcctgtgt atctggttta tcagcgaaaa gtataagggg taaacaagga taaagtgtca
    13501 ctctttagct aaccttgcat cgcattgacc aaaacttgaa ccgatttagc aaaacgtggc
    13561 atcggtcaat tcattcattt gacttatact tgcctgggca atattatccc ctgcaactaa
    13621 ttacttgcca gggcaactaa tgtgaaaagt accagcgatc tgttcaatga aattattcca
    13681 ttgggtcgtt taatccatat ggttaatcag aagaaagatc gcctgcttaa cgagtatctg
    13741 tctccgctgg atattaccgc ggcacagttt aaggtgctct gctctatccg ctgtgcggcg
    13801 tgtattactc cggttgaact gaaaaaagtg ttgtcggtcg acctgggggc actgacccgt
    13861 atgctggatc gcctggtctg taaaggctgg gtggaaaggt tgccgaaccc gaatgataag
    13921 cgcggcgtac tggtaaaact taccaccagc ggcgcggcaa tatgtgaaca atgccatcaa
    13981 ttagttggcc aggacctgca tcaagaatta acaaaaaacc tgacggcgga cgaagtggca
    14041 acacttgagc atttgcttaa gaaagtcctg ccgtaaacga aaaaagaggt atgacgatgt
    14101 ccagacgcaa tactgacgct attaccattc atagcatttt ggactggatc gaggacaacc
    14161 tggaatcgcc actgtcactg gagaaagtgt cagagcgttc gggttactcc aaatggcacc
    14221 tgcaacggat gtttaaaaaa gaaaccggtc attcattagg ccaatacatc cgtagccgta
    14281 agatgacgga aatcgcgcaa aagctgaagg aaagtaacga gccgatactc tatctggcag
    14341 aacgatatgg cttcgagtcc caacaaactc tgacccgaac cttcaaaaat tactttgatg
    14401 ttccgccgca taaataccgg atgaccaata tgcagggtga atcgcgcttt ttacatccat
    14461 taaatcatta caacaactag ttgataacgt gacaacgtca ctgaggcaat catgaaacca
    14521 cttttatccg caatagcaac tgcgcttatt ctcttttctg cgcagggcgt tgcggaacaa
    14581 accacgcagc cggttgttac ttcctgtggc aatgtcgtgg ttgttccccc atcgcaggaa
    14641 caaccaccgt ttgatttaaa tcacatgggt actggcagtg ataagtcgga tgcgctcggc
    14701 gtgccctatt ataatcaaca cgctatgtag tttgttctgg ccccgacatc tcggggctta
    14761 ttaacttccc acttttaccg ctttacgcca ccgcaagcca aatacattga tatacagccc
    14821 ggtcataatg agcaccgcac cgaaaaattg cagacccgtt aagcgttcat ccagcaatag
    14881 tgccgcactt gccagtccaa ctacaggcac cagtaacgat aacggtgcaa cccgccaggt
    14941 ttcatagcgt ccaagcagtg ttccccagat cccataacca acaattgtcg ctacaaatgc
    15001 cagatacatc agagacaaga tggtggtcat atcgatagta accagactgt gaatcatggt
    15061 tgcggaacca tcgagaatca gcgaggcagc aaagaaggga atgattggga ttaatgcgct
    15121 ccagattacc agcgacatca ccgccggacg cgttgcgtgc gacatgatct ttttattgaa
    15181 gatgttgcca cacgcccaac taaatgctgc cgccagggtc aacataaagc cgagcatcgc
    15241 cacatgctga ccgttcagac tatcttcgat taacaccagt acgccaaaaa tcgctaaggc
    15301 gatccccgtc aattgtttgc catgcagtcg ctcaccgaaa gtaaacgcgc caagcacgat
    15361 agtaaaaaac gcctgtgcct gtaacaccag cgaagccagt ccagcaggca taccaaagtt
    15421 aatggcgcag aacagaaaag caaactgcgc aaaactgatg gttaatccat accccagcag
    15481 caaattcagt ggtactttcg gtcgtgcgac aaaaaagatg gccgggaaag caaccagcat
    15541 aaagcgcaaa ccggccagca tcagcggtgg catgttatga agccccactt tgatgaccac
    15601 aaaatttagc ccccatacaa ccactaccag tagcgccaac accccatctt ttcgcgacat
    15661 tctaccgcct ctgaatttca tcttttgtaa gcaatcaact tagctgaatt tacttttctt
    15721 taacagttga ttcgttagtc accgtttacg acggcattaa tgcgcaaata agtcgctata
    15781 cttcggattt ttgccatgct attcctttac atctctaaaa caaaacataa cgaaacgcac
    15841 tgccggacag acaaatgaac ttatccctac gacgctctac cagcgccctt cttgcctcgt
    15901 cgttgttatt aaccatcgga cgcggcgcta cgctgccgtt tatgaccatt tacttgagtc
    15961 gccagtacag cctgagtgtc gatctaatcg gttatgcgat gacaattgcg ctcactattg
    16021 gcgtcgtttt tagcctcggt tttggtatcc tggcggataa gttcgacaag aaacgctata
    16081 tgttactggc aattaccgcc ttcgctagcg gttttattgc cattccttta gtgaataacg
    16141 tgacgctggt ggtgctcttt tttgccctta ttaactgcgc ctactccgtt tttgctaccg
    16201 tgctgaaagc ctggtttgcc gacaatcttt cgtccaccag caaaacgaag attttctcaa
    16261 tcaactacac catgctcaac attggctgga ccatcggtcc gccactcggc acgctgttgg
    16321 tgatgcagag catcaatctg cccttctggc tggcagctat ctgttccgcg tttcccatgc
    16381 ttttcattca aatttgggta aagcgcagcg agaaaatcat cgccacggaa acaggcagtg
    16441 tctggtcgcc gaaagtttta ttacaagata aagcactgtt gtggtttacc tgctctggct
    16501 ttctggcttc ttttgttagc ggcgcatttg cttcatgcat ttcacaatat gtgatggtga
    16561 ttgctgatgg tgattttgcc gaaaaggtgg tcgcggttgt tcttccggtg aatgctgcca
    16621 tggtagttac gttgcaatat tccgtaggcc gccgccttaa cccggctaac atccgcgcgc
    16681 tgatgacagc aggcaccttc tgtttcgtca tcgggctggt cggttttatt ttttccggca
    16741 acagcctgct aatgtggggt atgtcagctg cggtatttac tgtcggtgaa atcatttatg
    16801 cgccgggcga gtatatgttg attgaccata ttgcgccgcc aggaatgaaa gccagctatt
    16861 tttccgccca gtctttaggc tggcttggtg ctgcgattaa cccattagtg agtggcatag
    16921 tacttaccag tctgccgcct ttctcgctgt ttatcatctt agcgttggtg atcgttgttg
    16981 cgtgggtgct gatgttaaaa ggaattcgtg caagaccgtg ggggcagccc gcgctttgtt
    17041 gatttaagtc gaacacaata agaatttaat tcagccttcg tttaggttac ctctgctaat
    17101 atctttctca ttgagatgaa aattaaggta agcgaggaaa cacaccacac cataaacgga
    17161 ggcaaataat gctgggtaat atgaatgttt ttatggccgt actgggaata attttatttt
    17221 ctgggtttct ggccgcgtat ttcagccaca aatgggatga ctaatgaacg gagataatcc
    17281 ctcacctaac cggccccttg ttacagttgt gtacaagggg cctgattttt atgacggcga
    17341 aaaaaaaccg ccagtaaacc ggcggtgaat gcttgcatgg atagatttgt gttttgcttt
    17401 tacgctaaca ggcattttcc tgcactgata acggatcgtt gacacagtag catcagtttt
    17461 ctcaatgaat gttaaacgga gcttaaactc ggttaatcac attttgttcg tcaataaaca
    17521 tgcagcgatt ccttccggtt tgcttaccct catacattgc cctgtccgct cttccaatga
    17581 ccacatccag aggctcttca ggaaatgcac gactcacacc agcagtcacg gtaatgttga
    17641 tatgcccttc agaatgtgtg atggcatggt tatcgactaa ctggcaaatt ctgacacctg
    17701 cacgacatgc ttcttcatca ttagtcgctt tgacaataat gataaattct tcgcccccgt
    17761 agcgataaac cgtttcgtaa tcgcgcgtcc aactggctaa gtaagttgcg agggtgcgta
    17821 atactacatc gccgattaaa tgcccgtagg tatcattaac caatttaaat cggtctatat
    17881 ccaacaacat taaataaaga ttcagaggtt cagcattgcg taactgatga tcgaaggatt
    17941 catcaagaac ccgacgaccc ggcaatcccg tcagaacatc catattgcta cggaccgtaa
    18001 gcaaataaat tttgtaatcg gttaatgccg cagtaaaaga aagcaaccct tcctgaaaag
    18061 cgtcgaaatg cgcgtcctgc cagtgatttt caacaatagc cagcattaat tcccgaccac
    18121 agttatgcat atgttgatgg gcagaatcca ttagccgaac gtaaggtaat tcatcgttat
    18181 cgagtggccc cagatgatca atccaccgac caaactggca cagtccataa gaatggttat
    18241 ccgttatttc tggcttactg gcatctctcg cgaccacgct gtgaaacata cttaccagcc
    18301 actggtagtg ggcatcgata gccttattga gatttaacaa gatggcatca atttccgttg
    18361 tcttcttgat cattgccact cctttttcac agttccttgt gcgcgctatt ctaacgagag
    18421 aaaagcaaaa ttacgtcaat attttcatag aaatccgaa
//
LOCUS       DAHHTG010000087        18202 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.087, whole genome shotgun sequence.
ACCESSION   DAHHTG010000087 DAHHTG010000000
VERSION     DAHHTG010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18202)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18202)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18202
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.087"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            complement(363..953)
                     /locus_tag="NBI74_003082"
     CDS             complement(363..953)
                     /locus_tag="NBI74_003082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001260226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1933372.1"
                     /translation="MRTPSRYIFRLPSHEINPFRATLLLILLICAVLAGVSWLILSFV
                     RTGNTFIFWLTLFIGYLIAIAKQEKIKLIEKRQIMADKRQGLSICQFARQFSPHTVDT
                     WVIRAVWNTLQGNGYIDYPLPLKASDKLDDDLDLVNDADELEELVEDIAARCGRDLRG
                     IEDNPFLPITTVGSLVSVLNAQPMTQERRSLLFTRS"
     gene            complement(1003..2199)
                     /gene="hemY"
                     /locus_tag="NBI74_003083"
     CDS             complement(1003..2199)
                     /gene="hemY"
                     /locus_tag="NBI74_003083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX biogenesis protein HemY"
                     /protein_id="HCD1933373.1"
                     /translation="MLKVLLLFVLLIAGIVVGPMIAGHQGYVLIQTDNYNIETSVTGL
                     AIILILAMVVLFAIEWLLRRIFRTGAHTRGWFVGRKRRRARKQTEQALLKLAEGDYQQ
                     VEKLMAKNADHAEQPVVNYLLAAEAAQQRGDEARANQHLERAAELAGNDTIPVEITRV
                     RLQLARNENHAARHGVDKLLEVTPRHPEVLRLAEQAYIRTGAWSSLLDIIPSMAKAHV
                     GDEEHRAMLEQQAWIGLMDQARADNGSEGLRNWWKNQSRKTRHQVALQVAMAEHLIEC
                     DDHDTAQQIIIDGLKRQYDDRLLLPIPRLKTNNPEQLEKVLRQQIKNVGDRPLLWSTL
                     GQSLMKHGEWQEASLAFRAALKQRPDAYDYAWLADALDRLHKPEEAAAMRRDGLMLTL
                     QNNPPQ"
     gene            complement(2202..3437)
                     /gene="hemX"
                     /locus_tag="NBI74_003084"
     CDS             complement(2202..3437)
                     /gene="hemX"
                     /locus_tag="NBI74_003084"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HCD1933374.1"
                     /translation="MTEQEKTSAVVEETREAVDTTSQPVATEKKSKNNTALILSAVAI
                     AIALAAGVGLYGWGKQQAVNQTATSDALANQLTALQKAQESQKAELEGIIKQQAVQLE
                     QANRQQETLAKQLDEVQQKVATISGSDAKTWLLAQADFLVKLAGRKLWSDQDVTTAAA
                     LLKSADASLADMNDPSLITVRRAITDDIASLSAVSQVDYDGIILKLNQLSNQVDNLRL
                     ADNDSDGSPMDSDGEELSSSISEWRINLQKSWQNFMDNFITIRRRDDTAVPLLAPNQD
                     IYLRENIRSRLLVAAQAVPRHQEETYRQALENVSTWVRAYYDTDDATTKAFLDEVDQL
                     SQQNISMDLPETLQSQAMLEKLMQTRVRNLLAQPAAGTTEAKPAPAPAPAPAPAPAPA
                     PAPAPAPQADAPAAAPQGE"
     gene            complement(3459..4199)
                     /gene="hemD"
                     /locus_tag="NBI74_003085"
     CDS             complement(3459..4199)
                     /gene="hemD"
                     /locus_tag="NBI74_003085"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HCD1933375.1"
                     /translation="MSILVTRPSPAGEELVSRLRTLGQVAWHFPLIEFSPGRQLPQLA
                     DQLAALGESDLLFALSQHAVAFAQSQLHQQDRKWPRLPTYFAIGRTTALALHTVSGQK
                     ILYPQDREISEVLLQLPELQNIAGKCALILRGNGGRELIGDSLTARGAEVTFCECYQR
                     CAIHYDGAEEAMRWQSREVTTVVVTSGEMLQQLWSLIPQWYREHWLLRCRLLVVSERL
                     AKLARELGWQDIKVADNADNDALLRALQ"
     gene            complement(4196..5137)
                     /gene="hemC"
                     /locus_tag="NBI74_003086"
     CDS             complement(4196..5137)
                     /gene="hemC"
                     /locus_tag="NBI74_003086"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HCD1933376.1"
                     /translation="MLDNVLRIATRQSPLALWQAHYVKDKLMASHPGLVVELVPMVTR
                     GDVILDTPLAKVGGKGLFVKELEVALLENRADIAVHSMKDVPVEFPQGLGLVTICERE
                     DPRDAFVSNNYDSLDALPAGSIVGTSSLRRQCQLAERRPDLIIRSLRGNVGTRLSKLD
                     NGEYDAIILAVAGLKRLGLESRIRAALPPEISLPAVGQGAVGIECRLDDARTRELLAA
                     LNHHETALRVTAERAMNTRLEGGCQVPIGSYAELIDGEIWLRALVGAPDGSLIIRGER
                     RGAPQDAEQMGISLAEELLNNGAREILAEVYNGDAPA"
     gene            5524..8070
                     /gene="cyaA"
                     /locus_tag="NBI74_003087"
     CDS             5524..8070
                     /gene="cyaA"
                     /locus_tag="NBI74_003087"
                     /EC_number="4.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate cyclase"
                     /protein_id="HCD1933377.1"
                     /translation="MYLYIETLKQRLDAINQLRVDRALAAMGPAFQQVYSLLPTLLHY
                     HHPLMPGYLDGNVPKGICLYTPDETQRHYLNELELYRGMSVQDPPKGELPITGVYTMG
                     STSSVGQSCSSDLDIWVCHQSWLDSEERQLLQRKCSLLESWAASLGVEVSFFLIDENR
                     FRHNESGSLGGEDCGSTQHILLLDEFYRTAVRLAGKRILWNMVPCDEEEHYDDYVMTL
                     YAQGVLTPNEWLDLGGLSSLSAEEYFGASLWQLYKSIDSPYKAVLKTLLLEAYSWEYP
                     NPRLLAKDIKQRLHDGEIVSFGLDPYCMMLERVTEYLTAIEDFTRLDLVRRCFYLKVC
                     EKLSRERACVGWRRAVLSQLVSEWGWDEARLAMLDNRANWKIDQVREAHNELLDAMMQ
                     SYRNLIRFARRNNLSVSASPQDIGVLTRKLYAAFEALPGKVTLVNPQISPDLSEPNLT
                     FIYVPPGRANRSGWYLYNRAPNIESIISHQPLEYNRYLNKLVAWAWFNGLLTSRTRLY
                     IKGNGIVDLPKLQEMVADVSHHFPLRLPAPTPKALYSPCEIRHLAIIVNLEYDPTAAF
                     RNQVVHFDFRKLDVFSFGENQNCLVGSVDLLYRNSWNEVRTLHFNGEQSMIEALKTIL
                     GKMHQDAAPPDSVEVFCYSQHLRGLIRTRVQQLVSECIELRLSSTRQETGRFKALRVS
                     GQTWGLFFERLNVSVQKLENAIEFYGAISHNKLHGLSVQVETNHVKLPAVVDGFASEG
                     IIQFFFEETQDENGFNIYILDESNRVEVYHHCEGSKEELVRDVSRFYSSSHDRFTYGS
                     SFINFNLPQFYQIVKVDGREQVIPFRTKSIGNMPPANQEHDAPLLQQYFS"
     gene            complement(8110..8430)
                     /gene="cyaY"
                     /locus_tag="NBI74_003088"
     CDS             complement(8110..8430)
                     /gene="cyaY"
                     /locus_tag="NBI74_003088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000999950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron donor protein CyaY"
                     /protein_id="HCD1933378.1"
                     /translation="MNDSEFHRLADQLWLTIEEHLDDWDGDSDIDCEINGGVLTITFE
                     NGSKIIINRQEPLHQVWLATKQGGYHFDLKGDEWICDRSGETFWDLLEQAATQQAGET
                     VSFR"
     gene            complement(8556..8741)
                     /locus_tag="NBI74_003089"
                     /pseudo
     CDS             complement(8556..8741)
                     /locus_tag="NBI74_003089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000580503.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DKNYY family protein"
     gene            8900..9103
                     /locus_tag="NBI74_003090"
     CDS             8900..9103
                     /locus_tag="NBI74_003090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCD1933379.1"
                     /translation="MKNVFKALTVLLTLFSLTGCGLKGPLYFPPADKNAPPPTKPVET
                     QTQSTVPDKNDRATGDGPSQVNY"
     gene            9140..9964
                     /gene="dapF"
                     /locus_tag="NBI74_003091"
     CDS             9140..9964
                     /gene="dapF"
                     /locus_tag="NBI74_003091"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HCD1933380.1"
                     /translation="MQFSKMHGLGNDFMVVDAVTQNVFFSPELIRRLADRHLGVGFDQ
                     LLVVEPPYDPELDFHYRIFNADGSEVAQCGNGARCFARFVRLKGLTNKRDIRVSTANG
                     RMVLTVTDDDLVRVNMGEPNFEPSAVPFRANKAEKTYIMRAAEQTILCGVVSMGNPHC
                     VIQVDDVDTAAVETLGPVLESHERFPERANIGFMQVVKREHIRLRVYERGAGETQACG
                     SGACAAVAVGIQQGLLAEEVRVELPGGRLDIAWKGPGHPLYMTGPAVHVYDGFIHL"
     gene            9961..10668
                     /locus_tag="NBI74_003092"
     CDS             9961..10668
                     /locus_tag="NBI74_003092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF484 domain-containing protein"
                     /protein_id="HCD1933381.1"
                     /translation="MKQPGEELQETLTELDDRAVVDYLIKNPEFFIRNARAVEAIRVP
                     HPVRGTVSLVEWHMARARNHIHVLEENMALLMEQAIANEGLFYRLLYLQRSLTAASSL
                     DDMLMRFHRWARDLGLAGASMRLFPDRWRLGAPSNHTHLALSRQSFEPLRIQRLGQEQ
                     HYLGPLNGPELLVVLPEAKAVGSVAMSMLGSDADLGVVLFTSRDASHYQQGQGTQLLH
                     EIALMLPELLARWIERV"
     gene            10665..11561
                     /gene="xerC"
                     /locus_tag="NBI74_003093"
     CDS             10665..11561
                     /gene="xerC"
                     /locus_tag="NBI74_003093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HCD1933382.1"
                     /translation="MTDLHTDVERYLRYLSVERQLSPITLLNYQRQLEAIIHFASENG
                     LQSWQQCDVTMVRNFAVRSRRKGLGAASLALRLSALRSFFDWLVSQNELKANPAKGVS
                     APKAPRHLPKNIDVDDMNRLLDIDINDPLAVRDRAMLEVMYGAGLRLSELVGLDIKHL
                     DLESGEVWVMGKGSKERRLPIGRNAVAWIEHWLDLRDLFGSEDDALFLSKLGKRISAR
                     NVQKRFAEWGIKQGLNNHVHPHKLRHSFATHMLESSGDLRGVQELLGHANLSTTQIYT
                     HLDFQHLASVYDAAHPRAKRGK"
     gene            11561..12277
                     /gene="yigB"
                     /locus_tag="NBI74_003094"
     CDS             11561..12277
                     /gene="yigB"
                     /locus_tag="NBI74_003094"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase YigB"
                     /protein_id="HCD1933383.1"
                     /translation="MRFYRPLGRISALTFDLDDTLYDNRPVILRTEREALTFVQNYHP
                     ALRSFQNEDLQRLRQAVREAEPEIYHDVTRWRFRSIEQAMLDAGLSAEEASAGAHAAM
                     INFAKWRSRIDVPQQTHDTLKQLAKKWPLVAITNGNAQPELFGLGHYFEFVLRAGPHG
                     RSKPFSDMYFLAAEKLNVPIGEILHVGDDLTTDVGGAIRSGMQACWIRPENGDLMQTW
                     DSRLLPHLEISRLASLTSLI"
     gene            12361..14523
                     /gene="uvrD"
                     /locus_tag="NBI74_003095"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
     CDS             12361..14523
                     /gene="uvrD"
                     /locus_tag="NBI74_003095"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase II"
                     /protein_id="HCD1933384.1"
                     /translation="MDVSYLLDSLNDKQREAVAAPRSNLLVLAGAGSGKTRVLVHRIA
                     WLMSVENCSPYSIMAVTFTNKAAAEMRHRIGQLMGTSQGGMWVGTFHGLAHRLLRAHH
                     MDANLPQDFQILDSEDQLRLLKRLIKAMNLDEKQWPPRQAMWYINSQKDEGLRPHHIQ
                     SYGNPVEQTWQKVYQAYQEACDRAGLVDFAELLLRAHELWLNKPHILQHYRERFTNIL
                     VDEFQDTNNIQYAWIRLLAGDTGKVMIVGDDDQSIYGWRGAQVENIQRFLNDFPGAET
                     IRLEQNYRSTSNILSAANALIENNNGRLGKKLWTDGADGEPISLYCAFNELDEARFVV
                     NRIKTWQDNGGALAECAILYRSNAQSRVLEEALLQASMPYRIYGGMRFFERQEIKDAL
                     SYLRLIANRNDDAAFERVVNTPTRGIGDRTLDVVRQTSRDRQLTLWQACRELLQEKAL
                     AGRAASALQRFMELIDALAQETADMPLHVQTDRVIKDSGLRTMYEQEKGEKGQTRIEN
                     LEELVTATRQFSYNEEDEDLMPLQAFLSHAALEAGEGQADTWQDAVQLMTLHSAKGLE
                     FPQVFIVGMEEGMFPSQMSLDEGGRLEEERRLAYVGVTRAMQKLTLTYAETRRLYGKE
                     VYHRPSRFIGELPEECVEEVRLRATVSRPVSHQRMGTPMVENDSGYKLGQRVRHAKFG
                     EGTIVNMEGSGEHSRLQVAFQGQGIKWLVAAYARLETV"
     gene            complement(14578..15480)
                     /locus_tag="NBI74_003096"
     CDS             complement(14578..15480)
                     /locus_tag="NBI74_003096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosyl-l-methionine hydroxide
                     adenosyltransferase family protein"
                     /protein_id="HCD1933385.1"
                     /translation="MKKTLATLFLLTCLGSSSAYADNALILQTDFSLKDGAVSAMKGV
                     AFGVDHNLKIFDLTHEIPPYNIWEGAYRLYQTASYWPQGSVFVSVVDPGVGTDRKSVV
                     LKTKNGQYFVSPDNGTLTLVAESLGIESVRQIDEKTNRLKGSEKSYTFHGRDVYAYTG
                     ARLASGAITFEQVGPELPAKVVELSYQKAKATKGEVKGNIPILDIQYGNVWSNISDEL
                     LNQAGIKLNDTLCVTISEGSQQKYAGKMPYVASFGDVPEGQPMVYLNSLLNVSVALNM
                     DNFAQKHQVASGADWNIDVKKCAK"
     gene            complement(15563..16327)
                     /locus_tag="NBI74_003097"
     CDS             complement(15563..16327)
                     /locus_tag="NBI74_003097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HCD1933386.1"
                     /translation="MAHRLFIGKGMITLNLKRIFLTLTLLPLFAVAADDCALSDPTLT
                     VQAYTVNPQTERVKMYWQKANGEAWGTLHALLADINSQGQVQMAMNGGIYDESYAPLG
                     LYIENGQQKVALNLASGEGNFFIRPGGVFYVAGDKVGIVRLYAFKTSKEIQFAVQSGP
                     LLMENGVINPRIHPNVASRKIRNGVGINKHGNAVFLLSQQATNFYDFACYAKAKLNVE
                     QLLYLDGTISHMYMKGEAIPWQRYPFVTMISVEQKG"
     gene            16697..17647
                     /gene="corA"
                     /locus_tag="NBI74_003098"
     CDS             16697..17647
                     /gene="corA"
                     /locus_tag="NBI74_003098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HCD1933387.1"
                     /translation="MLSAFQLENNRLTRLEVEESQPLVNAVWIDLVEPDDDERLRVQS
                     ELGQSLATRPELEDIEASARFFEDDDGLHIHSFFFFEDAEDHAGNSTVAFTIRDGRLF
                     TLRERELPAFRLYRMRARSQSMVDGNAYELLLDLFETKIEQLADEIENIYSDLEQLSR
                     VIMEGHQGDEYDEALSTLAELEDIGWKVRLCLMDTQRALNFLVRKARLPGGQLEQARE
                     ILRDIESLLPHNESLFQKVNFLMQAAMGFINIEQNRIIKIFSVVSVVFLPPTLVASSY
                     GMNFEFMPELKWSFGYPGAIIFMILAGLAPYLYFKRKNWL"
     gene            complement(17690..18070)
                     /locus_tag="NBI74_003099"
     CDS             complement(17690..18070)
                     /locus_tag="NBI74_003099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000031247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2628 domain-containing protein"
                     /protein_id="HCD1933388.1"
                     /translation="MSKDYMADGSLTEKWKYRFSFYDEHGFPGFWGASPEYKQALKGL
                     NTRQKIIIRTNVIAFFFSWVYLFVLGLWKKAIVVLFLAIFSLIFGYMIGYNVFGFIIA
                     AYVSYNTNRWFYEKEVKGIHTWSL"
BASE COUNT         4244 a         4729 c         4799 g         4430 t
ORIGIN      
        1 tgacttttat tattcattat atatatgtgt agaattgtgc gcaggagaaa tattcactca
       61 ggaagttatt actcaggaag caaagaggat tacagaatta tctcataaca agtgttaagg
      121 gatgttattt cccggttctc tgtggcataa taaacgagta gatgctcatt ccatctctta
      181 tgttcgcctt agtgcctcat aaactccgga atgacgcaga gccgtttacg gtgcttatcg
      241 tccactgaca gatgtcgctt ctgcctcatc agacaccatg gacacaacgt tgagtgaagc
      301 acccacttgt tgtcatacag acctgtttta acgcctgctc cgtaataaga gcaggcgttt
      361 ttttatgacc gagtaaacaa caggctacgt cgctcttggg tcatcggttg tgcgtttaat
      421 acacttacaa gagaacctac cgtggtgatg ggaagaaacg gattatcttc tattccgcgt
      481 agatcgcgcc cacaacgagc ggcaatatct tcgaccagct cttctaactc gtctgcatcg
      541 tttactaaat ccagatcatc gtccagttta tcactggctt tcagtggtaa tggataatcg
      601 atatagccgt ttccttgtaa ggtattccac acagcccgaa tcacccatgt atctacggtg
      661 tggggggaaa attggcgtgc gaactgacaa atggaaagtc cctgacgttt atcagccata
      721 atctggcgct tctcgatgag cttgattttt tcctgcttcg caatggctat cagataccca
      781 ataaaaagtg ttaaccagaa gatgaatgta ttgcctgtac gtacgaagga cagaatcagc
      841 caacttacgc cagccagaac ggcacatatc aacaaaatca gcaagagcgt tgcccgaaaa
      901 ggattaattt catgtgatgg taagcggaaa atatagcggg acggtgtgcg catgtttgac
      961 atccttgtaa aaaaatatcg cccctgaacg aggcgatata gattactgcg gtgggttgtt
     1021 ttgcaacgtt agcatcaaac cgtcgcgacg catcgctgcg gcttcttccg gcttgtgcag
     1081 tctgtccagc gcgtcggcaa gccatgcgta atcgtaggcg tccggacgtt gtttcagcgc
     1141 tgcgcggaag gcgagcgatg cttcctgcca ttctccgtgt ttcatcagcg actgacccag
     1201 tgtgctccac aacagcgggc gatcgcccac gtttttgatc tgttggcgca gcactttttc
     1261 cagctgttcc ggattattgg ttttcagacg cgggatcggc agcagcaggc gatcgtcata
     1321 ctggcgtttc aggccgtcga tgataatttg ctgggcagta tcatgatcgt cacactcaat
     1381 aagatgttcc gccattgcca cctgcaaggc cacctgatga cgcgttttcc ggctttggtt
     1441 tttccaccag ttacgcaaac cttcgctacc gttatcggca cgcgcctgat ccatcaggcc
     1501 aatccatgcc tgttgttcca gcattgcacg atgttcttca tcaccaacat gggctttcgc
     1561 cattgatggg atgatatcca gcagcgaact ccatgcccct gtgcggatat acgcctgttc
     1621 cgccagacgt aatacttccg gatggcgtgg cgtaacttcc agcagcttat caacgccgtg
     1681 acgtgctgca tggttttcat tacgggccag ttgcagacgt acacgggtga tttctaccgg
     1741 aatggtgtca ttgccggcca gctccgctgc gcgttccaga tgttggttgg cgcgtgcttc
     1801 atcaccacgt tgttgcgcgg cttcggctgc cagtagatag ttcaccaccg gttgttcagc
     1861 gtgatcggca tttttcgcca tcagcttttc aacttgctga taatcgcctt ccgccagttt
     1921 cagcagcgcc tgttcggtct gcttacgtgc acggcgacgc ttacgtccga caaaccaccc
     1981 acgggtgtgc gcgcctgtgc ggaagattcg ccgcagtagc cactcaatgg caaacagcac
     2041 taccatcgcc agaatcaaaa taatcgcgag gcccgtgacg ctagtttcaa tattgtagtt
     2101 gtcggtctgg atcagcacgt aaccctgatg gccggcaatc atcgggccaa ccacgatccc
     2161 cgcaatcagc aacacaaaga gcaataacac ttttagcatg gttattctcc ttgcggcgcg
     2221 gctgccggag catcagcttg cggtgcaggt gcaggtgcag gtgcaggtgc aggtgcaggt
     2281 gcaggtgcag gtgcaggtgc aggtgcaggt ttagcttccg ttgtccctgc tgccggttgt
     2341 gccagcaggt tacgcacgcg ggtctgcatc aatttttcca gcatcgcctg gctttgcagg
     2401 gtttccggaa gatccatcga gatattttgc tggcttaact ggtccacctc gtcgaggaac
     2461 gctttggtgg tggcatcatc agtatcgtag taagcacgta cccaggtgga gacgttctcc
     2521 agcgcctggc gataagtctc ttcctggtga cgcggtacag cttgtgctgc gaccagcagg
     2581 cgagagcgga tattttcgcg cagatagata tcctgatttg gcgctaacag cggtacggca
     2641 gtgtcatcac gacggcgaat cgtaatgaag ttgtccataa aattctgcca gcttttttgc
     2701 agattgatac gccattcgct gatggaactg gaaagctctt caccgtcgga atccatcggc
     2761 gaaccatcgc tgtcattatc ggccagacgc agattatcta cctgatttga aagctgatta
     2821 agcttaagga tgataccgtc ataatccacc tgcgataccg cagaaaggct ggcgatatca
     2881 tcggtaattg cccggcgaac ggtaatcaga ctcgggtcat tcatatccgc caggctggcg
     2941 tctgcacttt tcagcaacgc tgcagcggtc gtgacgtcct gatcgctcca cagcttccgt
     3001 ccggcgagtt tcaccagaaa atcggcctgg gccagcagcc aggttttagc atcgctgccg
     3061 gaaatggtgg cgaccttttg ttggacttca tccagctgtt ttgccagcgt ttcttgctga
     3121 cgattcgcct gctcaagctg tacagcttgt tgcttgataa tgccttccag ctcggctttt
     3181 tggctctcct gggctttttg cagtgccgta agttggttag ccagggcatc gctggtggca
     3241 gtctgattga cggcctgttg tttaccccag ccatacaaac cgacgcccgc cgccagagca
     3301 atagcgatag ccaccgcgct gagaatcaat gcggtattgt tcttactctt tttttctgtt
     3361 gcgacaggtt gtgacgtggt gtccacggcc tccctggtct cttcaaccac ggcggaggtt
     3421 ttttcttgtt ccgtcattat ggcttcctgt tatgagagtt attgtaatgc ccgtaaaagc
     3481 gcatcgttgt cagcgttatc ggcgacctta atgtcttgcc agcccagttc ccgggcgagt
     3541 ttcgccaaac gctcactgac gaccaatagt cgacagcgta gtaaccagtg ctcacgatac
     3601 cattgtggga tcagcgacca gagttgctgc aacatttcac cgctggtaac aacgaccgtc
     3661 gtcacctcgc gggattgcca gcgcatcgct tcttctgcac catcgtaatg gattgcgcat
     3721 cgttgataac attcacaaaa agtgacctca gcaccgcgcg ccgtcaggga atccccaatt
     3781 agctcacgac cgccattgcc acgtaatatc agcgcacatt tgcccgcaat attttgtaat
     3841 tcaggtaatt gtagcaagac ttcgctgatt tcccgatcct gtgggtagag aatcttctgt
     3901 ccgcttacgg tatgtagtgc cagcgcggtg gtgcgtccaa tggcgaaata agtaggtagt
     3961 cggggccatt tacgatcttg ctgatgcagc tgtgattggg caaaagcaac cgcgtgttgc
     4021 gagagggcaa acaacagatc gctctctccc agcgctgcca gttgatcagc aagttgcggt
     4081 aattgtcgac ccggagaaaa ctcaatcagc ggaaaatgcc aggccacctg ccccagtgtg
     4141 cgcagacggc tcactaactc ttctcctgcg ggagacgggc gggtgaccag gatactcatg
     4201 ccggggcgtc tccgttatag acttcagcga ggatctcgcg cgcgccgtta ttcagtagct
     4261 cttctgccag cgaaatcccc atttgttcgg catcttgcgg cgcaccgcgg cgttcaccgc
     4321 gaataatcag cgaaccgtcc ggcgcgccga ccaacgcacg cagccagatt tcgccatcaa
     4381 taagctcggc gtagctacca attggcacct gacatccgcc ttcgagacgg gtattcatgg
     4441 cgcgttcggc ggtaacacgc agtgcagttt cgtggtgatt cagcgcggcg agcagctcgc
     4501 gagtgcgtgc atcatcaagg cggcattcaa tgcccaccgc accttgccct accgccggaa
     4561 gagaaatctc gggtggcaac gcggcgcgaa tgcgggactc cagacctaaa cgttttagtc
     4621 cggctacggc aagaatgatg gcatcgtatt caccgttatc cagtttgctc aggcgagtgc
     4681 cgacgttgcc gcgcagggag cggataatca gatccgggcg gcgttcagcc agttggcact
     4741 ggcgacgtaa actggacgtc ccgacgatac tgcctgccgg taacgcatcc agactgtcat
     4801 agttattgga cacaaatgca tcgcgaggat cttcacgctc gcaaatagtg accagcccca
     4861 gaccttgcgg gaattcaacc ggcacatctt tcatcgaatg tacggcgata tcggcgcgat
     4921 tttcgaggag cgcgacttcc agctctttaa caaataagcc ttttccgcct actttcgcca
     4981 gcggcgtatc aagaatcaca tcgccgcgcg tcaccatcgg taccagttca acgaccaggc
     5041 ccggatggct cgccatcaac ttgtctttga catagtgtgc ctgccagagt gcaagtgggc
     5101 tttggcgtgt ggcaattctt aaaacattgt ctaacatgct tgttaccgtc attatcatcc
     5161 gtggtccatc ctaacatcct tgccagagcg atgtcagtgt tgtggtgaaa cgtagacgcc
     5221 tgcgcaaacc gtaaaatgag gtcgggcagt ggatcctgac aggcgctttg agccttcgta
     5281 ataaggaatt tacagagaat aaacggtgct acacttgtat gtagcgcatc tttctttacg
     5341 gtcaatcagc aaggtgttaa attgatcacg ttttagacca ttttttcgtc gtgaaactaa
     5401 aaaaaccagg cgcgaaaagt ggtaacggtt acctttgaca tacgaaatat cccgaatgcc
     5461 gcgtgttacc gttgatgttg gcggaatcac agtcatgacg ggtagcaaat caggcgatac
     5521 gtcttgtacc tctatattga gactctgaaa cagagactgg atgccataaa tcaattgcgt
     5581 gtggatcgcg cgcttgctgc tatggggcct gcattccaac aggtctacag tctactgccg
     5641 acattgttgc actatcacca tccgctaatg ccgggttacc ttgatggtaa cgttcccaaa
     5701 ggcatttgcc tttacacgcc tgatgaaact caacgccact acctgaacga gcttgaactg
     5761 tatcgtggaa tgtcagtaca ggacccaccg aaaggtgagc ttccaattac tggtgtatac
     5821 accatgggca gcacctcgtc cgtagggcaa agttgttcct ctgacctgga tatctgggtc
     5881 tgtcatcaat cctggctcga tagcgaagag cgccagttgc tacaacgtaa atgtagtctg
     5941 ctggaaagct gggccgcctc gctgggtgtg gaagtcagct tcttcctgat tgatgaaaac
     6001 cgcttccgtc ataatgaaag cggcagcctg gggggcgaag attgtggctc cacccagcat
     6061 atactgctgc ttgacgaatt ttatcgtacc gccgtgcgtc tcgccggtaa gcgtattctg
     6121 tggaatatgg tgccgtgcga cgaagaagag cattacgacg actatgtgat gacgctttac
     6181 gcgcagggcg tgctgacgcc aaatgaatgg ctggatcttg gtggcttaag ctcgctttct
     6241 gctgaagagt actttggtgc cagcctttgg cagctctaca agagtatcga ttccccatac
     6301 aaagcggtgc tgaaaacact gctgctggaa gcctattcct gggaataccc gaacccacgt
     6361 ctgctggcga aagatatcaa acagcgtttg cacgacggcg agattgtatc gtttggcctc
     6421 gacccgtact gcatgatgct ggagcgtgtt actgaatacc tgacggcgat tgaagatttc
     6481 acccgtctgg atttagtgcg tcgctgcttc tatttaaaag tgtgcgaaaa gctcagccgt
     6541 gaacgcgcct gtgtaggctg gcgtcgcgca gtgttgagcc agttagtgag cgagtggggt
     6601 tgggacgaag ctcgtctggc aatgctcgat aaccgcgcta actggaagat agatcaggtg
     6661 cgtgaggcgc acaacgagct gctcgacgca atgatgcaga gctaccgtaa tctgatccgc
     6721 tttgcgcgcc gcaataacct tagcgtctcc gccagcccgc aggatatcgg cgtgctgaca
     6781 cgtaaactgt acgccgcgtt tgaagcatta ccgggtaaag tgacgctggt aaacccgcag
     6841 atttcgcccg atctctcgga accgaatctg acctttattt atgtaccgcc gggccgcgcc
     6901 aaccgttcag gttggtatct gtataaccgc gcgccaaata ttgagtcaat catcagccat
     6961 cagccgctgg aatataatcg ctacctgaat aaactggtgg cgtgggcatg gtttaacggc
     7021 ctgctgacct ctcgcacccg tttgtatatt aaaggtaacg gcattgtcga tttgcctaag
     7081 ttgcaggaga tggtcgcaga cgtgtcgcac catttcccgc tgcgcttacc tgcaccgaca
     7141 ccgaaggcgc tctacagccc gtgtgagatc cgccatctgg cgattatcgt taacctggaa
     7201 tatgacccga cggcagcctt ccgcaatcag gtggtgcatt ttgatttccg taagctggat
     7261 gtcttcagct ttggcgagaa tcaaaattgc ctggtgggta gcgttgacct gctgtatcgc
     7321 aactcgtgga acgaagtgcg tacgctgcac ttcaacggtg agcagtcgat gattgaagcc
     7381 ctgaaaacca ttctcggcaa aatgcatcag gacgccgcac cgccagatag cgtggaagtc
     7441 ttctgctaca gccagcatct gcgcggctta atccgtactc gcgtgcagca actggtttct
     7501 gagtgtattg aactgcgtct ttccagcacc cgccaggaaa ccgggcgctt caaggcgctg
     7561 cgcgtctccg gtcaaacctg gggcttgttc ttcgaacggc tgaatgtctc ggtacagaaa
     7621 ctggaaaacg ccatcgagtt ttatggcgct atttcgcata acaaactgca cggcctgtca
     7681 gtgcaggttg aaaccaatca cgtcaaatta ccggcggtgg tggacggctt tgccagcgaa
     7741 gggatcatcc agttcttctt cgaagaaacg caagacgaga atggctttaa tatctacatt
     7801 ctcgacgaaa gcaaccgggt tgaggtgtat caccactgcg aaggcagcaa agaggaactg
     7861 gtgcgtgacg tcagtcgctt ctactcgtct tcgcacgacc gtttcaccta cggctcaagc
     7921 ttcatcaact tcaacctgcc gcagttctat cagattgtga aggttgatgg tcgtgaacag
     7981 gtgattccgt ttcgcaccaa atctatcggt aacatgccac ctgccaatca ggagcatgac
     8041 gcgccgctgt tgcagcagta tttttcttga tgctaatgcc ggaaagcgag gcttatccgg
     8101 cattagctct taacgaaaac tgacggtttc tcccgcctgc tgcgtcgccg cctgttccag
     8161 caaatcccag aaggtttcgc cgctgcgatc gcaaatccac tcatcgcctt tcagatcaaa
     8221 atggtagccg ccctgtttgg ttgccagcca tacctggtgc agtggttcct ggcggttgat
     8281 aatgattttg ctgccgttct caaaggtaat ggttagtaca ccgccgttga tttcacagtc
     8341 gatatcgctg tcgccatccc agtcgtccag atgttcttca atggtcagcc acagttgatc
     8401 agccaggcga tgaaattcac tgtcgttcat tgttgtatcc tgtttttaag tgatggcggc
     8461 agtatagcgg catggggtca gggcttcaaa gtttgcccgt ctgctgctgc gttccggcat
     8521 gattcatccg tcaccggaat aataatatct ctatatagcg aaagattggt cttttcataa
     8581 gggcgcaaat acaccacgcc ttttccctgt ttctggtaaa tattatgttc cggctcatca
     8641 agccgacatc cccatgccga cgataagaac aacgaacaag acaataaaca aaccacattg
     8701 cgatagtgca taaagccatc ctggcgcgag gtgccgatca cgaaactacc agcaaaacat
     8761 aaatcgccac gagtaagcgt tatactcgca gcatttcctc acttttcaga cttcataaag
     8821 agtcgctaaa cgcttgcttt tacgccttct cctgcgatga tagaaagcag aaagcgatga
     8881 actttacagg caatccataa tgaaaaacgt gtttaaggca ctcactgtat tacttactct
     8941 cttcagcctg acgggctgcg gtctgaaagg tccgctctat ttcccgcctg cagataaaaa
     9001 cgcaccgccg ccgaccaaac cggtagagac gcaaacgcaa tccacggtac cggacaaaaa
     9061 cgaccgcgcc actggcgatg gtccatccca ggtgaattac taaaagtcag tttctgtacc
     9121 cgcgtgattg gagtaaatga tgcagttctc gaaaatgcat ggccttggca acgattttat
     9181 ggtcgtcgac gcggtaacgc agaatgtctt tttttcaccg gagctgattc gtcgcctggc
     9241 ggatcggcac ctgggggtag ggtttgacca attgctggtg gttgagccac cgtatgatcc
     9301 ggaactggat tttcactatc gcattttcaa tgctgatggc agtgaagtgg cgcagtgcgg
     9361 caacggcgcg cgctgctttg cccgttttgt gcgcctgaaa gggctgacca ataagcgcga
     9421 tatccgcgtc agcaccgcca acgggcggat ggttctgacc gtcaccgatg atgatctggt
     9481 ccgcgtaaat atgggcgaac ccaacttcga accttctgcc gtgccatttc gcgctaacaa
     9541 agcggaaaag acctatatta tgcgcgccgc cgagcagaca atcttatgcg gcgtggtgtc
     9601 gatgggaaat ccgcactgcg tgattcaggt tgatgatgtt gatactgcgg cggtagaaac
     9661 gcttggtcct gttctggaaa gccacgaacg ttttccggag cgcgccaata tcggttttat
     9721 gcaagtggtt aaacgcgagc atattcgttt acgcgtttat gagcgtgggg caggagaaac
     9781 ccaggcctgc ggcagcggcg cgtgtgcggc ggtggcggta gggattcagc aaggtttgct
     9841 ggccgaagaa gtacgcgtgg aactccctgg cggtcgtctt gatatcgcct ggaaaggtcc
     9901 gggtcacccg ttatatatga ctggcccggc ggtacatgtc tacgacggat ttattcatct
     9961 atgaagcaac caggggaaga actgcaggaa acactcacgg agcttgatga ccgggcggtt
    10021 gtcgattatc tgattaaaaa tcctgagttt tttatccgca atgcgcgcgc agtagaagcg
    10081 atacgcgtgc cgcatccggt acgcggtacc gtttcgttgg tcgagtggca catggcccgc
    10141 gcacgtaatc atattcatgt tctggaagag aacatggcgt tgttgatgga acaggctatc
    10201 gccaacgaag gcctgtttta tcgtctactc tacctgcagc gcagtctcac cgccgccagc
    10261 agtctcgatg atatgctgat gcgctttcac cgctgggcgc gcgatctcgg cctggcaggt
    10321 gcgagtatgc gcctgtttcc agatcgctgg cgcttaggtg cgccgtcgaa ccacactcat
    10381 ctggcattaa gccgtcagtc tttcgaaccg ctgcgtattc agcgtctggg gcaggaacag
    10441 cactatctgg gaccgcttaa cggaccggag ctgctggtgg tgctaccgga agcgaaagcg
    10501 gtgggatcgg tggcgatgtc gatgctggga agcgatgctg atttgggggt tgtgctgttt
    10561 accagtcgcg atgccagtca ctatcaacaa gggcagggaa cgcagttact tcatgaaatt
    10621 gcgctgatgt tgccggagct tctggcgcgt tggattgaac gcgtatgacc gatttacaca
    10681 ccgatgtaga acgctaccta cgttatctga gcgtggagcg ccagcttagc ccgataaccc
    10741 tgcttaacta ccagcgacag cttgaggcga tcatccattt tgccagcgaa aacggcctgc
    10801 aaagctggca gcaatgtgat gtgacgatgg tgcgtaattt tgctgtacgc agtcgccgta
    10861 aagggctggg agctgcaagt ctggcgttac ggctttctgc gctacgtagc ttttttgact
    10921 ggctggtcag ccagaatgaa ctcaaagcta acccggcgaa aggtgtttcg gcaccgaaag
    10981 cgccgcgtca tctgccgaaa aacatcgacg tcgatgatat gaatcggctg ctggatattg
    11041 atatcaacga tcccctcgcc gtgcgcgatc gggcaatgct ggaagtgatg tacggcgcgg
    11101 gtctgcgtct ttccgagctg gtggggctcg atatcaaaca cctcgacctg gagtctggcg
    11161 aagtgtgggt gatgggaaaa ggcagcaaag agcgccgcct gccgattggt cgcaacgccg
    11221 tggcgtggat tgagcactgg cttgatttgc gcgacctgtt tggtagtgaa gacgacgcgc
    11281 ttttcctgtc gaaactgggc aaacgcatct ccgcgcgtaa tgtgcagaaa cgctttgccg
    11341 aatggggcat aaaacaaggg ctgaataatc acgttcaccc gcataaatta cgtcactcgt
    11401 tcgctaccca tatgctggag tcgagcggcg atcttcgtgg tgtgcaggag ctgctgggtc
    11461 atgccaacct ctccaccacg caaatctata ctcatcttga ttttcaacac cttgcctcgg
    11521 tgtacgatgc ggcgcatcca cgcgccaaac gggggaaata atgcgttttt accggccttt
    11581 ggggcgcatc tcggcgctca cctttgacct ggatgatacc ctttacgata accgtccggt
    11641 gattttgcgc accgagcgag aggcgcttac ctttgtgcaa aattatcatc cggcgctgcg
    11701 cagcttccag aatgaagatc tgcaacgcct gcgccaggcg gtacgggaag cggaacctga
    11761 gatttatcac gacgtgacgc gctggcgttt tcgttcgatt gaacaagcga tgctcgacgc
    11821 cgggctgagt gccgaagaag ccagtgcagg cgcacatgca gcaatgatca actttgccaa
    11881 atggcgcagc cggatcgacg tcccgcagca aacccacgac accttaaaac agctggcgaa
    11941 gaaatggccg ctggtggcga tcaccaacgg taacgcccag ccggaactgt ttggtttggg
    12001 gcattatttt gagtttgtgc tgcgcgctgg cccgcacggg cgctcaaaac cgttcagcga
    12061 tatgtacttc ctggcggcgg aaaaactcaa cgtgccgatc ggcgagatct tacatgtcgg
    12121 ggacgatctc accactgacg tgggtggggc aattcgcagc ggaatgcagg cctgttggat
    12181 cagaccggaa aatggcgatc tgatgcaaac ctgggacagc cgtttactgc cgcatctgga
    12241 aatttcccgg ttggcatctc tgacctcgct gatataatca gcaaatctgt atatataccc
    12301 agctttttgg cggagggcgt tgcgcttctc cgcccaacct atttttacgc ggcggtgcca
    12361 atggacgttt cttacctgct cgacagcctt aatgacaaac agcgcgaagc ggtggccgcg
    12421 ccacgcagca accttctggt gctggcgggc gcgggcagtg gtaagacgcg cgtactggtg
    12481 catcgtatcg cctggttgat gagcgtggaa aactgctcgc cgtattcgat tatggcggtg
    12541 acgtttacca acaaagcggc ggcggagatg cgtcatcgta tcgggcaact gatgggcact
    12601 agccagggcg gtatgtgggt cggcaccttc cacgggctgg cgcaccgcct gctgcgtgcg
    12661 caccatatgg acgccaacct gccgcaggat ttccagatcc tcgacagtga agaccagcta
    12721 cgtctgctta agcgtttgat caaggcgatg aatctcgacg agaagcagtg gccgccgcgt
    12781 caggcaatgt ggtacatcaa cagccagaaa gatgaggggc tgcgtccgca tcatattcag
    12841 agctacggta atccggtgga gcagacctgg cagaaggtgt atcaggcgta tcaggaagcg
    12901 tgtgaccgcg cgggcctggt ggacttcgcc gagctgctgc tacgtgctca cgagttgtgg
    12961 cttaacaagc cgcatatcct tcagcactat cgcgaacggt ttaccaatat cctggtggac
    13021 gaattccagg ataccaacaa cattcagtat gcgtggatcc gcctgctggc gggcgatacc
    13081 ggcaaagtga tgatcgttgg cgatgacgat cagtcaatct acggctggcg cggagcgcag
    13141 gtggagaata ttcagcgttt cctcaatgat ttccccggtg ctgaaactat ccgtctggag
    13201 cagaactacc gctctaccag caatattctg agcgccgcga acgccctgat tgaaaacaac
    13261 aacgggcgtt tgggtaaaaa actgtggacc gatggcgcgg acggtgagcc gatttccctt
    13321 tattgcgctt ttaacgaact cgacgaagcg cgttttgtgg ttaatcgcat caaaacctgg
    13381 caggacaacg gcggggcgct cgctgagtgc gccattctct accgcagcaa cgcccagtcg
    13441 cgtgtgctgg aagaggcgtt attgcaggcg agtatgccgt atcgtattta cggcgggatg
    13501 cgcttcttcg aacgccagga aatcaaagat gcgctctcgt atctgcgcct gattgccaac
    13561 cgcaacgacg acgcggcctt tgagcgtgtg gtgaatacgc caacgcgggg tattggtgac
    13621 cggacgctgg acgtggtacg tcagacatcg cgagatcgcc agttaacact ctggcaggca
    13681 tgtcgtgaac tgttgcagga aaaagccctc gccggacgtg ctgccagcgc cttacagcgg
    13741 tttatggagc tgatcgacgc cttagcgcag gaaactgccg atatgccgct gcatgtacag
    13801 actgaccggg taattaaaga ctccggcctg cgcaccatgt acgagcagga gaagggcgaa
    13861 aaaggtcaga cgcgtatcga aaacttagag gaactggtga cggcaacgcg ccagttcagc
    13921 tacaacgaag aagacgaaga tttaatgccg ctgcaggcat tcctctccca tgcggcgctg
    13981 gaagcgggcg aggggcaggc ggatacctgg caggatgcgg tgcagttgat gacgctacac
    14041 tcggcgaaag gtctggaatt cccgcaggtg tttatcgttg gtatggaaga gggcatgttc
    14101 ccaagccaga tgtcgctgga tgaaggcggg cgtctggaag aagaacgccg tctggcctac
    14161 gttggcgtaa cccgcgcgat gcagaaactg acgctgacct acgcggaaac ccgccgtctg
    14221 tatggcaaag aggtttacca tcgcccgtcg cgctttatcg gtgagttgcc ggaagagtgt
    14281 gtggaagagg tgcgcctgcg cgccacggta agccgcccgg tcagccatca gcgtatgggt
    14341 acgccgatgg tcgagaacga cagcggctac aagctcggcc agcgcgtacg ccacgctaag
    14401 tttggtgaag gaaccatcgt caatatggaa ggcagcggcg agcatagccg tttgcaggtg
    14461 gcattccagg gccagggtat taaatggctg gtggcggctt acgcccgact ggagacggta
    14521 taatttacga ttccgttgcc ggatgcggtg cgccacaccg catccggcat cagtaactta
    14581 cttagcgcac ttcttcacat caatattcca gtcagcacca gacgcaacct gatgtttctg
    14641 cgcgaaatta tccatattca gcgccacgga aacattcaat aagctgttca gatacaccat
    14701 cggctggcct tccggcacat cgccgaagct tgcgacatac ggcattttcc ctgcgtattt
    14761 ttgctgggaa ccttcggaaa tggtcacaca caacgtgtcg ttgagtttga ttcctgcctg
    14821 attcagcaac tcgtcgctga tattgctcca gacgttgccg tactgaatat cgagaatcgg
    14881 gatattgccc ttcacttcac cttttgttgc tttggctttc tgataagaaa gttcgacaac
    14941 ttttgcagga agctctggcc ccacctgctc gaacgtgatc gccccggaag ccagacgtgc
    15001 accggtgtag gcatacacgt cacgaccgtg gaaggtatat gacttttcgg aacctttcag
    15061 acggttggtt ttttcatcga tttgccggac gctctcaata cccagcgatt ccgccaccaa
    15121 cgtgagcgta ccgttatctg gtgaaacgaa gtactgaccg tttttggttt tcagcactac
    15181 cgatttacgg tctgtaccaa cacctggatc gaccacgcta acaaacaccg aaccttgcgg
    15241 ccagtaactg gcagtctgat acaggcgata agcgccttcc cagatgttat acggtggaat
    15301 ttcgtgtgtt aaatcaaaga ttttaagatt atggtcaaca ccgaatgcga cccctttcat
    15361 cgccgagacg gcaccatctt tcaggctaaa atccgtttgt aaaatcaaag cgttatccgc
    15421 ataagcggat gatgacccca gacaggttaa taaaaataat gttgcgagcg tttttttcat
    15481 ttttgtttcc cttcatttat ctgatgattt tccagctgtg tagccagccc cagtttgtct
    15541 ggcctcctcg agtgtacaat gtttaacctt tctgctcgac ggaaatcatg gtgacaaaag
    15601 gatagcgttg ccacgggatt gcctcgcctt tcatatacat atgtgaaatt gtgccatcaa
    15661 gataaagcag ctgctcaacg ttcagtttcg ctttggcata acaggcaaaa tcataaaaat
    15721 ttgtcgcctg ctggctcagc aaaaacacgg cgttcccatg tttattaatc ccaacaccgt
    15781 tacgaatttt acgtgaggcg acgttgggat gaatacgcgg attaattaca ccgttttcca
    15841 tcaacaatgg ccctgactgc accgcaaact gaatctcttt actggtttta aaggcataca
    15901 gacgaacgat gccgacttta tctcccgcga cataaaacac tccgccagga cgaataaaga
    15961 aattcccttc acctgaagcg agatttaacg ccaccttctg ctgaccgttt tcgatgtaca
    16021 aaccgagcgg cgcatagctt tcatcataga tgccgccgtt catcgccatc tgcacctgac
    16081 cctgactatt aatatccgcc agcagagcat gtaacgttcc ccacgcttcg ccattggctt
    16141 tttgccagta cattttcacc cgctctgttt gaggattaac ggtatacgcc tgtacggtca
    16201 gcgtcggatc tgagagtgcg caatcatcag cggcaacagc aaacaaggga agtaaggtga
    16261 gggtgagaaa aatccgtttg agattcaagg tgatcattcc tttaccaatg aatagccgat
    16321 gcgccattat aggccctgaa tgtgggattt ttttatcctg ttagcgacct tgacgagtac
    16381 caaaaagcgc gaagttcaac tattgttctg tggtgttctg ttgcgtgttg acggcaaaat
    16441 tttgctggcg taacatgcgc gcacgatcac tctaagagga cattcgcctt ggacacaccc
    16501 agtagatact ggctcattat cctgtcatcc aggatcaact cctaaggcta tccctttttg
    16561 ctgatagcct tagcggttgt cagcgacctc aatttttccc gtcgcgctga gtcaggctgt
    16621 ttaatggtct gaaacccaat ttgtttctgt gtgcccaccg aactgtccga tattttaagc
    16681 attgggagtc ccggtcatgc tgagcgcatt tcaactggaa aataaccgac tgacccggct
    16741 ggaagtcgaa gagtcacaac cccttgtaaa tgcagtatgg attgatcttg tcgaaccgga
    16801 cgacgacgag cgactgcgcg tacaatctga actcggccag agcctggcaa cccgcccgga
    16861 actggaagac atcgaagcat cggcacgttt ctttgaagat gatgacggcc tgcatattca
    16921 ctccttcttc ttctttgaag atgcggaaga tcacgccggt aactccactg tggcatttac
    16981 catccgtgat ggtcgtctgt ttactctgcg tgagcgtgaa ttgcctgctt ttcgccttta
    17041 ccgtatgcgt gcccgtagcc agtcgatggt agacggtaac gcctacgagc tgctgctgga
    17101 tctgttcgaa accaaaatcg aacagttggc ggatgaaatt gaaaacatct acagcgatct
    17161 ggagcagttg agccgggtta ttatggaagg gcatcagggc gatgagtatg acgaggcgct
    17221 ctccacactg gcggaactgg aagatatcgg ctggaaagtt cgcctgtgtc tgatggatac
    17281 ccagcgtgcg ctgaacttcc tggtgcgtaa ggcgcgttta ccgggcgggc aactggagca
    17341 ggcgcgtgaa atcctgcgag atatcgaatc cctgctgccg cataacgaat ctctattcca
    17401 gaaggtgaac ttcctgatgc aggcggcgat gggttttatc aacatcgagc agaaccgcat
    17461 catcaaaatc ttctcggtgg tatccgtcgt gttcctgcca ccgacgctgg tcgcttccag
    17521 ctacgggatg aactttgagt ttatgccaga actgaagtgg agcttcggct acccaggtgc
    17581 gattatcttt atgatcctcg cgggcctggc accgtatctg tactttaagc ggaagaactg
    17641 gctgtaaaaa acgagaacgg tggcttagcc tggctaagcc accgattatt taaaggctcc
    17701 aggtatgaat accttttact tctttttcat agaaccatct gttggtattg tatgaaacat
    17761 aagcggcgat aatgaatcca aatacattat agccaatcat gtaaccaaaa attaaagaaa
    17821 atatcgccag gaatagaaca acaatagcct tcttccataa acccaataca aataaataga
    17881 cccacgagaa gaaaaaagca ataacatttg ttctaattat tatcttttgc cttgtgttta
    17941 atccttttaa tgcttgttta tattctgggc ttgcacccca aaatccaggg aagccatgtt
    18001 catcataaaa cgaaaagcgg tacttccact tttcagttaa tgagccatct gccatataat
    18061 ccttactcat aaaatctcca ttaattattt acggggggaa tcagtggggg caagaactaa
    18121 catgccaaag gtggaaaaat aacaaatcaa aaaaatcatc gaaccattgc cagaacagtc
    18181 aaaatgctca gcaaccatga tg
//
LOCUS       DAHHTG010000088        18080 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.088, whole genome shotgun sequence.
ACCESSION   DAHHTG010000088 DAHHTG010000000
VERSION     DAHHTG010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18080)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18080)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18080
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.088"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            complement(229..975)
                     /gene="cutC"
                     /locus_tag="NBI74_003100"
     CDS             complement(229..975)
                     /gene="cutC"
                     /locus_tag="NBI74_003100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HCD1933389.1"
                     /translation="MALLEICCYSMECALTAQQNGADRVELCAAPKEGGLTPSLGVLK
                     SVRQRVTIPVHPIIRPRGGDFCYSDGEFAAILEDVRTVRELGFPGLVTGVLDVDGNVD
                     MPRMEKIMAAAGPLAVTFHRAFDMCANPLNTLNNLTELGIARVLTSGQKSDALQGLSK
                     IMELIAHRDAPIIMAGAGVRAENLHHFLDAGVLEVHSSAGAWQASPMRYRNQGLSMSS
                     DAHADEYSRYVVDGAAVAEMKGIIERHQAK"
     gene            complement(989..1555)
                     /locus_tag="NBI74_003101"
     CDS             complement(989..1555)
                     /locus_tag="NBI74_003101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCD1933390.1"
                     /translation="MANWQSIDELQDIASDLPRFTHALDELSRRLGLDITPLTADHIS
                     LRCHQNVTAERWRRGFEQCGELLSENMINGRPICLFKLHEPLQVAHWQFSIVELPWPG
                     EKRYPHEGWEHIEIVLPGDPETLNARALALLSDEGLSLPGISVKTSSPKGEHERLPNP
                     TLAVTDGKTTIKFHPWSIEEIVASEQSA"
     gene            1771..3504
                     /gene="argS"
                     /locus_tag="NBI74_003102"
     CDS             1771..3504
                     /gene="argS"
                     /locus_tag="NBI74_003102"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HCD1933391.1"
                     /translation="MNIQALLSEKVRQAMIAAGAPADCEPQVRQSAKVQFGDYQANGM
                     MAVAKKLGMAPRQLAEQVLTHLDLNGIASKVEIAGPGFINIFLDPAFLAEHVQQALAS
                     DRLGVATPEKQTIVVDYSAPNVAKEMHVGHLRSTIIGDAAVRTLEFLGHKVIRANHVG
                     DWGTQFGMLIAWLEKQQQENAGEMELADLEGFYRDAKKHYDEDEEFAERARNYVVKLQ
                     SGDEYFREMWRKLVDITMTQNQITYDRLNVTLTRDDVMGESLYNPMLPGIVADLKAKG
                     LAVESEGATVVFLDEFKNKEGEPMGVIIQKKDGGYLYTTTDIACAKYRYETLHADRVL
                     YYIDSRQHQHLMQAWAIVRKAGYVPESVPLEHHMFGMMLGKDGKPFKTRAGGTVKLAD
                     LLDEALERARRLVAEKNPDMPADELEKLANAVGIGAVKYADLSKNRTTDYIFDWDNML
                     AFEGNTAPYMQYAYTRVLSVFRKAEINEEQLAAAPVIIREDREAQLAARLLQFEETLT
                     VVAREGTPHVMCAYLYDLAGLFSGFYEHCPILSAENEEVRNSRLKLAQLTAKTLKLGL
                     DTLGIETVERM"
     gene            complement(3557..3949)
                     /gene="flhe"
                     /locus_tag="NBI74_003103"
     CDS             complement(3557..3949)
                     /gene="flhe"
                     /locus_tag="NBI74_003103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlhE"
                     /protein_id="HCD1933392.1"
                     /translation="MRALLAILLFPLLVQAAGEGMWQASSVGVTLNHRGESMSSAPLS
                     TRQPASGLMTLVAWRYQLIGPTPAGLRVRLCSQSRCVELEGQSGTTMAFSGIPAAEPL
                     RFIWEVPGGGRLTPPLKIQRNEVIVNYR"
     gene            complement(3949..6027)
                     /gene="flhA"
                     /locus_tag="NBI74_003104"
     CDS             complement(3949..6027)
                     /gene="flhA"
                     /locus_tag="NBI74_003104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HCD1933393.1"
                     /translation="MSNLAAMLRLPANLKSTQWQILAGPILILLILSMMVLPLPAFIL
                     DLLFTFNIALSIMVLLVAMFTQRTLEFAAFPTILLFTTLLRLALNVASTRIILMEGHT
                     GAAAAGKVVEAFGHFLVGGNFAIGIVVFVILVIINFMVITKGAGRIAEVGARFVLDGM
                     PGKQMAIDADLNAGLIGEDEAKKRRSEVTQEADFYGSMDGASKFVRGDAIAGILIMVI
                     NVVGGLLVGVLQHGMSMGHAAESYTLLTIGDGLVAQIPALVISTAAGVIVTRVSTDQD
                     VGEQMVNQLFSNPSVMLLSAAVLGLLGLVPGMPNLVFLLFTAGLLGLAWWIRGREQKA
                     PAEPKPVKMAENNSVVEATWNDVQLEDSLGMEVGYRLIPMVDFQQDGELLGRIRSIRK
                     KFAQEMGFLPPVVHIRDNMDLQPARYRILMKGVEMGSGDAYPGRWLAINPGTAAGTLP
                     GEATVDPAFGLNAIWIESALKEQAQIQGYTVVEASTVVATHLNHLISQHAAELFGRQE
                     AQQLLDRVAQEMPKLTEDLVPGVVTLTTLHKVLQNLLDEKVPIRDMRTILETLAEHAP
                     IQSDPHELTAVVRVALGRAITQQWFPGKDEVHVIGLDTPLERLLLQALQGGGGLEPGL
                     ADRLLAQTQEALSRQEMLGAPPVLLVNHALRPLLSRFLRRSLPQLVVLSNLELSDNRH
                     IRMTATIGGK"
     gene            complement(6020..7168)
                     /gene="flhB"
                     /locus_tag="NBI74_003105"
     CDS             complement(6020..7168)
                     /gene="flhB"
                     /locus_tag="NBI74_003105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FlhB"
                     /protein_id="HCD1933394.1"
                     /translation="MSDESDDKTEAPTPHRLEKAREEGQIPRSRELTSLLILLVGVSV
                     IWFGGVSLARRLSGMLSAGLHFDHSIINDPNLILGQIILLIREAMLALLPLISGVVLV
                     AIISPVMLGGLVFSGKSLQPKFSKLNPLPGIKRTFSAQTGAELLKAILKTILVGSVTG
                     FFLWYHWPQMMRLMAESPITAMGNAMDLVGLCALLVVLGVIPMVGFDVFFQIFSHLKK
                     LRMSRQDIRDEFKQSEGDPHVKGRIRQMQRAAARRRMMADVPKADVIVNNPTHYSVAL
                     QYDENKMSAPKVVAKGAGLVALRIREIGAENNVPTLEAPPLARALYRHAEIGQQIPGQ
                     LYAAVAEVLAWVWQLKRWRLAGGQRPVQPTHLPVPEALDFINEKPTHE"
     gene            complement(7357..8001)
                     /gene="cheZ"
                     /locus_tag="NBI74_003106"
     CDS             complement(7357..8001)
                     /gene="cheZ"
                     /locus_tag="NBI74_003106"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase CheZ"
                     /protein_id="HCD1933395.1"
                     /translation="MMQPSIKPADEHSAGDIIARIGSLTRMLRDSLRELGLDQAIAEA
                     AEAIPDARDRLYYVVQMTAQAAERALNSVEASQPHQDQMEKSAKALTQRWDDWFADPI
                     DLADARELVTDTRQFLADVPAHTSFTNAQLLEIMMAQDFQDLTGQVIKRMMDVIQEIE
                     RQLLMVLLENIPEQESRPKRENQSLLNGPQVDTSKAGVVASQDQVDDLLDSLGF"
     gene            complement(8012..8401)
                     /gene="cheY"
                     /locus_tag="NBI74_003107"
     CDS             complement(8012..8401)
                     /gene="cheY"
                     /locus_tag="NBI74_003107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002210873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator CheY"
                     /protein_id="HCD1933396.1"
                     /translation="MADKELKFLVVDDFSTMRRIVRNLLKELGFNNVEEAEDGVDALN
                     KLQAGGYGFVISDWNMPNMDGLELLKTIRADGAMSALPVLMVTAEAKKENIIAAAQAG
                     ASGYVVKPFTAATLEEKLNKIFEKLGM"
     gene            complement(8416..9465)
                     /gene="cheB"
                     /locus_tag="NBI74_003108"
     CDS             complement(8416..9465)
                     /gene="cheB"
                     /locus_tag="NBI74_003108"
                     /EC_number="3.1.1.61"
                     /EC_number="3.5.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate methylesterase/protein
                     glutamine deamidase"
                     /protein_id="HCD1933397.1"
                     /translation="MSKIRVLSVDDSALMRQIMTEIINSHSDMEMVATAPDPLVARDL
                     IKKFNPDVLTLDVEMPRMDGLDFLEKLMRLRPMPVVMVSSLTGKGSEVTLRALELGAI
                     DFVTKPQLGIREGMLAYSEMIAEKVRTAAKASLAAHKPLSVPTTLKAGPLLSSEKLIA
                     IGASTGGTEAIRHVLQPLPLSSPALLITQHMPPGFTRSFADRLNKLCQIGVKEAEDGE
                     RVLPGHAYIAPGDRHMELARSGANYQIKIHDGPAVNRHRPSVDVLFHSVAKQAGRNAV
                     GVILTGMGNDGAAGMLAMRQAGAWTLAQNEASCVVFGMPREAINMGGVCEVVDLSQVS
                     QQMLAKISAGQAIRI"
     gene            complement(9468..10328)
                     /gene="cheR"
                     /locus_tag="NBI74_003109"
     CDS             complement(9468..10328)
                     /gene="cheR"
                     /locus_tag="NBI74_003109"
                     /EC_number="2.1.1.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HCD1933398.1"
                     /translation="MTSSLPCGQTSLLLQMTERLALSDAHFRRISQLIYQRAGIVLAD
                     HKRDMVYNRLVRRLRALGQADFGHYLNLLESNQHSGEWQAFINSLTTNLTAFFREAHH
                     FPLLADHARRRSGEYRVWSAAASSGEEPYSIAMTLADTLGTAPGRWKVFASDIDTEVL
                     EKARSGIYRHEELKNLTPQQLQRYFMRGTGPHEGLVRVRQELANYVDFAPLNLLAKQY
                     TVPGPFDAIFCRNVMIYFDQTTQQAILRRFVPLLKPDGLLFAGHSENFSHLERRFTLR
                     GQTVYALSKD"
     gene            complement(10443..10573)
                     /locus_tag="NBI74_003110"
                     /pseudo
     CDS             complement(10443..10573)
                     /locus_tag="NBI74_003110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310622.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Tar ligand binding domain-containing protein"
     gene            complement(10619..12280)
                     /gene="tar"
                     /locus_tag="NBI74_003111"
     CDS             complement(10619..12280)
                     /gene="tar"
                     /locus_tag="NBI74_003111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein II"
                     /protein_id="HCD1933399.1"
                     /translation="MINRIRVVTLLVMVLGVFALLQLISGSLFFSSLHHSQKSFVVSN
                     QLREQQGELTSTWDLMLQTRINLSRSAVRMMMDSSNQQSNAKVELLDSARKTLAQAAT
                     HYKKFKSMAPLPEMVATSRNIDEKYKNYHTALTELIDYLDYGNTGAYFAQPTQGMQNA
                     MGEAFAQYALSSEKLYRDIVTDNADDYRFAQWQLAVIALVVVLILLVAWYGIRRMLLT
                     PLAKIIAHIREIAGGNLANTLTIDGRSEMGDLAQSVSHMQRSLTDTVTHVREGSDAIY
                     AGTREIAAGNTDLSSRTEQQASALEETAASMEQLTATVKQNADNARQASQLAQSASDT
                     AQHGGKVVDGVVKTMHEIADSSKKIADIISVIDGIAFQTNILALNAAVEAARAGEQGR
                     GFAVVAGEVRNLASRSAQAAKEIKALIEDSVSRVDTGSVLVESAGETMNNIVNAVTRV
                     TDIMGEIASASDEQSRGIDQVALAVSEMDRVTQQNASLVQESAAAAAALEEQASRLTQ
                     AVSAFRLAASPLTNKPQTPSRPASEQPPAQPRLRIAEQDPNWETF"
     gene            complement(12425..12928)
                     /gene="cheW"
                     /locus_tag="NBI74_003112"
     CDS             complement(12425..12928)
                     /gene="cheW"
                     /locus_tag="NBI74_003112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HCD1933400.1"
                     /translation="MTGMTNVTKLASEPSGQEFLVFTLGDEEYGIDILKVQEIRGYDQ
                     VTRIANTPAFIKGVTNLRGVIVPIVDLRIKFSQVDVDYNDNTVVIVLNLGQRVVGIVV
                     DGVSDVLSLTAEQIRPAPEFAVTLSTEYLTGLGALGDRMLILVNIEKLLNSEEMALLD
                     SAASEVA"
     gene            complement(12949..14913)
                     /gene="cheA"
                     /locus_tag="NBI74_003113"
     CDS             complement(12949..14913)
                     /gene="cheA"
                     /locus_tag="NBI74_003113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HCD1933401.1"
                     /translation="MSMDISDFYQTFFDEADELLADMEQHLLVLQPEAPDAEQLNAIF
                     RAAHSIKGGAGTFGFSVLQETTHLMENLLDEARRGEMQLNTDIINLFLETKDIMQEQL
                     DAYKQSQEPDAASFDYICQALRQLALEAKDETPSAVTRLSVVAKSEPQDEQSRSQSPR
                     RIILSRLKAGEVDLLEEELGHLTTLTDVVKGADSLSAILPGDIAEDDITAVLCFVIEA
                     DQITFETVDVSPKISTPPVLKLAAEQAPTGRVEREKTTRSSESTSIRVAVEKVDQLIN
                     LVGELVITQSMLAQRSSELDPVNHGDLITSMGQLQRNARDLQESVMSIRMMPMEYVFS
                     RYPRLVRDLAGKLGKQVELTLVGSSTELDKSLIERIIDPLTHLVRNSLDHGIELPEKR
                     LAAGKNSVGNLILSAEHQGGNICIEVTDDGAGLNRERILAKAASQGLTVSENMSDDEV
                     AMLIFAPGFSTAEQVTDVSGRGVGMDVVKRNIQEMGGHVEIQSKQGTGTTIRILLPLT
                     LAILDGMSVRVADEVFILPLNAVMESLQPREADLHPLAGGERVLEVRGEYLPIVELWK
                     VFNVAGAKTEATQGIVVILQSGGRRYALLVDQLIGQHQVVVKNLESNYRKVPGISAAT
                     ILGDGSVALIVDVSALQAINREQRMANTAA"
     gene            complement(14918..15844)
                     /gene="motB"
                     /locus_tag="NBI74_003114"
     CDS             complement(14918..15844)
                     /gene="motB"
                     /locus_tag="NBI74_003114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HCD1933402.1"
                     /translation="MKNQAHPIIVVKRRKAKSHGAAHGSWKIAYADFMTAMMAFFLVM
                     WLISISSPKELIQIAEYFRTPLATAVTGGDRISNSESPIPGGGDDYTQSQGEVNKRPN
                     IEELKKRMEQSRLRKLRGDLDQLIESDPKLRALRPHLKIDLVQEGLRIQIIDSQNRPM
                     FRTGSADVEPYMRDILRAIAPVLNGIPNRISLSGHTDDFPYASGEKGYSNWELSADRA
                     NASRRELMVGGLNGGKVLRVVGMAATMRLSDRGPDDAVNRRISLLVLNKQAEQAILHE
                     NAESQNEPVSALEKPEVAPQVSVPTMPSAEPR"
     gene            complement(15841..16728)
                     /gene="motA"
                     /locus_tag="NBI74_003115"
     CDS             complement(15841..16728)
                     /gene="motA"
                     /locus_tag="NBI74_003115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor stator protein MotA"
                     /protein_id="HCD1933403.1"
                     /translation="MLILLGYLVVLGTVFGGYLMTGGSLGALYQPAELVIIAGAGIGS
                     FIVGNNGKAIKGTLKALPLLFRRSKYTKAMYMDLLALLYRLMAKSRQMGMFSLERDIE
                     NPRESEIFASYPRILADSVMLEFIVDYLRLIISGHMNTFEIEALMDEEIETHESEAEV
                     PANSLALVGDSLPAFGIVAAVMGVVHALGSADRPAAELGALIAHAMVGTFLGILLAYG
                     FISPLASVLRQKSAETSKMMQCVKVTLLSNLNGYAPPIAVEFGRKTLYSSERPSFIEL
                     EEHVRAVKNPQQQTTTEEA"
     gene            complement(16855..17433)
                     /gene="flhC"
                     /locus_tag="NBI74_003116"
     CDS             complement(16855..17433)
                     /gene="flhC"
                     /locus_tag="NBI74_003116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhC"
                     /protein_id="HCD1933404.1"
                     /translation="MSEKSIVQEARDIQLAMELITLGARLQMLESETQLSRGRLIKLY
                     KELRGSPPPKGMLPFSTDWFMTWEQNVHASMFCNAWQFLLKTGLCNGVDAVIKAYRLY
                     LEQCPQAEEGPLLALTRAWTLVRFVESGLLQLSSCNCCGGNFITHAHQPVGSFACSLC
                     QPPSRAVKRRKLSQNPADIIPQLLDEQRVQAV"
     gene            complement(17436..17786)
                     /gene="flhD"
                     /locus_tag="NBI74_003117"
     CDS             complement(17436..17786)
                     /gene="flhD"
                     /locus_tag="NBI74_003117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416406.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhD"
                     /protein_id="HCD1933405.1"
                     /translation="MHTSELLKHIYDINLSYLLLAQRLIVQDKASAMFRLGINEEMAT
                     TLAALTLPQMVKLAETNQLVCHFRFDSHQTITQLTQDSRVDDLQQIHTGIMLSTRLLN
                     DVNQPEEALRKKRA"
BASE COUNT         4319 a         5012 c         4442 g         4307 t
ORIGIN      
        1 tgttatttag aatatatatt tgtcagtttt ggcaatttaa atcacataat gaatgtgaat
       61 atatagaata tttctagcac aatgctatgc gcactgtcac ccgctcaaca gatcacggca
      121 atcgttcgtt ttttatacta ctcagggata aaaaaaggcc cctgttgaaa ttgcaggggc
      181 ctggtacgag caagcatcat attgggcgac atgatgcaac ggtaaaaatc atttggcctg
      241 atggcgttca atgattcctt tcatttcagc aaccgccgcc ccgtctacga cataacgcga
      301 atactcgtcc gcgtgtgcat ctgatgacat ggacaatcct tgattacgat aacgcatcgg
      361 tgaggcttgc cacgctcccg cggagctatg gacttccagc actccggcat cgaggaagtg
      421 gtgcaggttt tccgcacgga ctcctgctcc ggccataatg attggagcat cacgatgggc
      481 aataagttcc ataatttttg ataaaccttg cagtgcgtct gatttttgcc ctgatgtcag
      541 tacccgggca atgcccaatt ccgtaagatt attgagtgta tttaaagggt tagcgcacat
      601 atcgaaggcg cgatgaaaag tcactgccag cggaccggca gcagccatta ttttttccat
      661 tcgtggcata tcgacattcc cgtcaacatc gagaacgccc gtcaccagtc caggaaaacc
      721 taattcgcgg actgtgcgca catcttcaag aatggcggca aactcaccgt cgctgtagca
      781 aaaatcacca ccgcgtgggc ggataatcgg atgcactggg atcgtcaccc gctggcgcac
      841 ggatttcagt acccccaacg acggcgttag gcccccctct tttggagctg cgcataattc
      901 aactctgtca gcgccgtttt gctgcgccgt tagcgcgcat tccatgctgt aacagcaaat
      961 ttccagtaat gccatttttg ctccttagtt acgccgactg ctcgctggca acgatctctt
     1021 caatggacca cggatgaaac ttaatggttg ttttaccatc ggtgaccgcc agcgtcggat
     1081 tcggtaatcg ttcatgctca ccttttggcg aactggtttt aactgagatc cccggcaggc
     1141 tgagtccttc atcagaaagc aatgccagcg cacgggcatt cagcgtttct ggatcacccg
     1201 gtagaacgat ttcaatatgt tcccaacctt cgtgcgggta acgtttttcc ccgggccacg
     1261 gtagctccac aatggaaaac tgccagtgcg caacctgtag cggttcatgc aatttaaaca
     1321 aacaaatcgg tctgccattg atcatatttt ctgacaaaag ctcgccgcac tgttcaaacc
     1381 cgcgacgcca gcgttcagcg gtgacgtttt gatggcaacg caaagaaatg tgatcggcag
     1441 tcagcggagt gatatccaac cccagacggc gggaaagttc atctaatgcg tgggtaaatc
     1501 gcggtaaatc cgatgcaata tcctgcagct cgtcgataga ttgccagttc gccataatca
     1561 ttcttcgtct ttcattaaaa gcgttaattt accctgttgc cctgcgccaa ccaaccgctg
     1621 atttcacgcc gcttctgatg caatagtgaa aacggcaata cgccacgcgc acgttgctga
     1681 cgaaaacagc catttgcagt atactcccgc cctaatttct ttaactggtg cgggtaattt
     1741 ttgctcgctt catcaatgta aggtattccg gtgaatattc aggctcttct ctcagaaaaa
     1801 gtccgtcagg ccatgattgc ggcaggcgcg cctgcggatt gcgaaccgca ggttcgtcag
     1861 tcagcaaaag ttcagttcgg cgactatcag gctaacggca tgatggcagt tgctaaaaaa
     1921 ctgggtatgg caccgcgaca attagcagag caggtgctga ctcatctgga tcttaacggt
     1981 atcgccagca aagttgagat cgccggtcca ggctttatca acattttcct tgatccggca
     2041 ttcctggctg aacatgttca gcaggcgctg gcgtccgatc gtctcggtgt tgctacgcca
     2101 gaaaaacaga ccattgtggt tgactactct gcgccaaacg tggcgaaaga gatgcatgtc
     2161 ggtcacctgc gctctaccat tattggtgac gcagcagttc gtactctgga gttcctcggt
     2221 cacaaagtga ttcgcgcaaa ccacgtcggc gactggggca ctcagttcgg tatgctgatt
     2281 gcatggctgg aaaagcagca gcaggaaaac gccggtgaaa tggagctggc tgaccttgaa
     2341 ggtttctacc gcgatgcgaa aaagcattac gacgaggatg aagagtttgc cgagcgtgca
     2401 cgtaactacg tggtaaaact gcaaagcggt gacgaatatt tccgcgagat gtggcgcaaa
     2461 ctggtcgaca tcaccatgac gcagaaccag atcacctacg atcgtctgaa cgtgacgttg
     2521 acccgcgatg acgtcatggg tgaaagtctc tacaacccaa tgctgccggg tatcgtggcg
     2581 gacctgaaag ccaaaggtct ggcagtagaa agtgaaggcg cgaccgttgt attccttgat
     2641 gagttcaaaa acaaggaagg cgaaccgatg ggcgttatca ttcagaagaa agatggcggc
     2701 tatctctaca ccaccactga tatcgcctgc gcgaaatatc gttatgaaac actgcatgcc
     2761 gatcgcgtgc tgtattacat cgattcccgt cagcatcaac acctgatgca ggcatgggcg
     2821 atcgtccgta aagcgggcta tgtaccggaa tccgtaccgc tggaacacca catgttcggc
     2881 atgatgctgg gcaaagatgg taaaccgttc aaaacccgcg ctggcggtac ggtgaaactg
     2941 gccgatctgc tggatgaagc cctggagcgt gcacgtcgtc tggtggcaga aaagaacccg
     3001 gatatgccag ccgacgagct ggaaaaactg gctaacgcgg ttggtattgg tgcggtgaaa
     3061 tatgcggatc tctccaaaaa ccgcaccacg gactacatct tcgactggga caacatgctg
     3121 gcgtttgagg gtaataccgc gccatacatg cagtatgctt acacgcgtgt attgtccgtg
     3181 ttccgtaaag cagaaattaa cgaagagcaa ctggctgcag ctccggtaat catccgtgaa
     3241 gatcgtgaag cgcaactggc agctcgcctg ctgcagtttg aagaaaccct cactgtggtt
     3301 gcccgtgaag gcacgccgca tgtgatgtgt gcttacctgt acgatctggc cggtctgttc
     3361 tctggcttct atgagcactg cccgatcctc agcgcagaaa acgaagaagt gcgtaacagc
     3421 cgtctgaaac tggcacaact gacggcgaag acgctgaagc tgggtctgga tacgctgggt
     3481 attgagacag tagagcgtat gtaatcgcgt tatacggcaa cagccgatgt cattgcagac
     3541 atcggctttt ttatcattaa cgataattta caatcacttc attccgttga atctttagcg
     3601 gcggagttag ccgcccaccg ccaggcactt cccagataaa gcgcaacggt tctgccgccg
     3661 gtataccgga aaaggccatg gtggttccac tctgcccctc caattcgaca caacgagatt
     3721 gtgaacacaa gcgaacccgc agtcctgcag gtgtcggacc gataagctga taacgccacg
     3781 ccaccagcgt catcaatcct gaagcaggtt gtcgcgtaga aagaggcgct gacgacatcg
     3841 attcaccgcg atgatttagc gtaacgccca cgctactcgc ctgccacatc ccctccccgg
     3901 cggcttgcac cagcagcgga aacaataata ttgctaataa ggctctcatt atttgccgcc
     3961 aatcgtcgcc gtcatacgga tatgtcggtt atctgatagt tccagattcg acagcaccac
     4021 taactgcggc aagctgcggc gcaggaagcg agacaataat ggtcgtagcg cgtggttcac
     4081 caacaatacc ggcggcgcac ccaacatctc ctgacgggat agcgcttcct gagtttgcgc
     4141 cagtaaacga tccgccagcc ctggctccag tccaccaccg ccttgcaacg cctgtagcaa
     4201 caaacgttcc agcggtgtat cgaggccaat aacatggact tcatctttgc caggaaacca
     4261 ctgctgggta atcgcccgtc ccaacgctac gcgcacgaca gcggttaatt catgcggatc
     4321 gctttggatg ggcgcatgtt ccgccagcgt ttcgagaatg gtgcgcatat cgcgaatcgg
     4381 cactttttca tcgagcagat tttgcagcac tttatgcagt gtggtaagcg tgacgacgcc
     4441 aggaacgaga tcttccgtca gctttggcat ctcctgggcg acgcgatcca gcagctgttg
     4501 tgcctcctga cgaccaaaca gctctgcggc atgctggcta atgaggtggt taagatgcgt
     4561 tgccaccacc gtgctggcct caaccacggt gtacccctga atctgcgcct gttctttaag
     4621 cgcactttca atccagatag cattcaggcc aaatgccgga tcaacggtcg cttcaccagg
     4681 taatgttccg gcggcagtgc ctggattaat tgccagccag cgccccggat aagcatcacc
     4741 actccccatt tcgacacctt tcatcaagat acgatagcgg gctggttgca aatccatgtt
     4801 atcgcggatg tgcaccactg ggggtaggaa gcccatctcc tgggcaaatt tcttgcggat
     4861 actgcgtata cggcccaaca actcaccatc ctgctgaaaa tcaaccatcg ggatcaaccg
     4921 ataccccact tccattccca gagaatcttc cagttgtaca tcgttccacg ttgcttcgac
     4981 taccgaatta ttctctgcca tttttaccgg ttttggttcg gcaggcgctt tttgttcacg
     5041 tccgcggatc caccaggcca gccccagcaa tccggcagtg aacagcaaaa ataccaggtt
     5101 tggcattcca ggcaccaggc cgagtaaacc gagcacggcg gcgcttaata acataacgct
     5161 tgggttactg aaaagctgat tcaccatctg ctcgccaaca tcctgatcgg tgctgacacg
     5221 cgtaacgatg acccccgcgg cggtagaaat caccaacgcc ggaatttgtg ccaccagacc
     5281 gtcgccaatg gtcaatagcg tataactttc cgccgcgtgc cccatgctca tgccatgttg
     5341 cagcacaccg accagcaatc cgccgacaac gttaatgacc atgatgagga tcccggcgat
     5401 ggcatcgccg cgaacaaact tacttgcccc gtccatcgag ccgtaaaaat cggcttcctg
     5461 agtcacttcg gagcggcgtt ttttcgcttc atcttcacca atcaatccgg cgttaaggtc
     5521 ggcgtcaatc gccatctgct tacccggcat accatcgaga acaaagcgcg cgcccacttc
     5581 cgcaatacgc ccggcacctt tggtgataac cataaagttg atgatcacga gaatgacgaa
     5641 caccacgata ccgatagcga aattgccacc gacgaggaag tgaccgaacg cttcgaccac
     5701 ctttccagcc gccgccgcgc cggtatgccc ttccatcaaa atgatacggg ttgaagccac
     5761 gttaagtgcc agacgcaaca gcgtggtaaa caacagaatg gtcggaaacg cagcaaactc
     5821 gagcgtgcgc tgggtaaaca tcgccaccag caacaccatg atcgacaagg caatattgaa
     5881 ggtaaacagc aggtcgagta tgaatgcggg cagtggcagc accatcatcg acaagatcaa
     5941 caggatcaaa atcggtccgg caaggatctg ccattgtgtc gatttcaggt ttgcgggcag
     6001 gcgcagcatc gcggccagat tactcatggg tcggtttctc gttaataaaa tccagggctt
     6061 ccggcaccgg aagatgagta ggttgtacag ggcgctgtcc accagccaga cgccagcgtt
     6121 tcagttgcca gacccaggcc agcacttccg ccaccgcggc gtacagttga cccgggattt
     6181 gttgaccgat ctccgcatgt cgatacagcg cacgcgccag cggcggcgct tcaagcgtcg
     6241 ggacgttatt ttcagcgcca atttcacgaa tgcgcagcgc gaccagccct gcacctttag
     6301 cgaccacttt cggtgcgctc attttgtttt cgtcatactg caacgctacc gaatagtggg
     6361 tcgggttatt gacaatgaca tccgctttcg gcacatcggc catcatccga cgccgtgcgg
     6421 ccgctcgctg catctgacgg atccgccctt taacatgggg gtcgccttcg ctttgtttga
     6481 actcatcacg aatatcctgc cgtgacattc gcagcttttt caggtggctg aagatttgga
     6541 aaaagacgtc aaatcccacc attggaatga caccaagcac caccagcagc gcgcatagcc
     6601 ctaccagatc cattgcatta cccatggcgg taatcggaga ctcggccatt aagcgcatca
     6661 tctgcggcca gtgataccag agaaaaaacc ccgtcacgct accaaccagg atggttttca
     6721 aaattgcctt aagcaactcc gcgccagtct gtgccgagaa cgtccgttta atgcccggta
     6781 gcgggttgag tttggaaaac ttcggctgca aggattttcc gctaaatacc agccccccca
     6841 gcataaccgg agaaataatc gccaccagca ccacgccgct aatcagcggt aacagcgcca
     6901 acatggcttc tctgatcagc agaataatct gcccgaggat cagattcggg tcattgataa
     6961 tactgtgatc aaaatgcagc cctgcggaga gcatgcccga caatcgacgg gccagcgaca
     7021 caccgccaaa ccagataaca ctaacgccca ccagcaaaat cagcagtgaa gtcagttcac
     7081 gggaacgcgg gatttgcccc tcttcccgcg ctttttctag tcggtgaggt gtgggggcct
     7141 ctgttttgtc gtcgctctcg tcagacacgt cgccaatcct ggaaagagta ttaagcgtaa
     7201 atgatgccag agcgcaaagc gttcaatggt ttgagtaagg ggcaaaacag gcgggattta
     7261 gggcttttac tgccacacat caagcatagt atgcgtttgt cggatgcggc gtcatcgcct
     7321 gatatgacgt ggtcacgtca catcaggcaa tacaaatcaa aatccaagac tatccaacaa
     7381 atcgtccacc tgatcctgac tggctaccac accggctttg ctggtatcga cctgaggtcc
     7441 attaagcaaa ctctggtttt cacgttttgg acgcgactcc tgttccggga tgttttccaa
     7501 cagcaccatc agcaactgac gttcgatctc ctgaatgaca tccatcatcc gcttaatgac
     7561 ctgcccggtg agatcctgaa aatcctgcgc catcatgatt tccagcagtt gcgcgttagt
     7621 aaagctggta tgcgcgggga catctgccag aaattgccgt gtatcggtca ccagttcacg
     7681 ggcgtcggca aggtcaatcg gatcggcaaa ccagtcatcc caacgttggg ttaacgcttt
     7741 tgctgatttc tccatttgat cctgatgcgg ttgtgacgcc tcaacactgt tcagcgcccg
     7801 ctccgcagcc tgggcggtca tctgcacaac atagtacaaa cgatcgcgcg catcggggat
     7861 ggcttccgct gcttcggcaa tggcctgatc cagccccagt tcccgcaaac tgtcgcgcag
     7921 catacgcgtc aggctgccga tgcgcgcaat gatatcgcca gctgaatgct cgtcagcagg
     7981 tttgattgat ggttgcatca tagtcgcatc ctcacatgcc cagtttctca aagattttgt
     8041 tgagtttttc ctccagcgtc gcggcggtaa atggcttcac cacatagcca ctggcccccg
     8101 cttgcgccgc agcaatgatg ttctctttct tcgcttctgc agtcaccatt aacactggca
     8161 atgccgacat cgcgccatcc gcacgaatcg ttttcagcaa ctccaggcca tccatattgg
     8221 gcatgttcca gtcggagata acaaatccat aaccgcctgc ctgcaactta ttgagagcgt
     8281 cgacgccatc ttccgcttcc tcaacattat tgaatcccag ctctttcagc aggttacgca
     8341 ctatgcgtcg catggtggaa aagtcatcca caaccaaaaa tttaagttct ttatccgcca
     8401 tttcacactc ctgatttaaa tacgtatcgc ctgtccggca ctaatttttg ccagcatttg
     8461 ctgacttacc tggctaagat cgaccacttc gcagacacca cccatattga tggcctcgcg
     8521 cggcatgccg aacaccacgc aacttgcttc gttttgcgca agggtccatg cccccgcctg
     8581 acgcatcgcc aacattcccg ccgcgccgtc gttgcccatg cccgtcagga tcaccccaac
     8641 cgcattacgc cccgcctgtt tggcgacaga atggaacaac acatctaccg atggccgatg
     8701 gcgattaacc gccgggccat cgtgaatttt gatttgataa ttcgcgccgc tacgcgccag
     8761 ctccatatgc cgatcgcccg gcgcgatata ggcatgtccc ggcaagacgc gctctccgtc
     8821 ttcggcttct ttaaccccga tctggcaaag cttattaagt ctgtcggcaa aagagcgggt
     8881 gaaaccgggc ggcatatgct gggtaattaa cagtgctgga ctggaaagcg gcaacggttg
     8941 cagtacgtga cgaattgcct cagttccacc cgttgaagca ccaatcgcaa tcagtttttc
     9001 agaactcaac aacggccctg ccttcagcgt tgtcggtacc gacaatggct tatgtgctgc
     9061 aaggctcgcc tttgctgccg tacgcacctt ttcagcaatc atttcgctat atgccagcat
     9121 tccttcgcga atacccagtt gcggtttggt gacaaaatct atcgccccca gctccagcgc
     9181 gcgcagcgtg acttctgacc ctttgccggt cagggaagaa accatcacaa cgggcattgg
     9241 acgcaaacgc attaattttt cgaggaaatc cagtccgtcc atccgcggca tttcaacatc
     9301 cagcgtcaat acatcgggat tgaatttctt aatcaaatca cgcgcaacca gcggatcagg
     9361 ggcggtcgcc accatttcca tgtcgctatg gctgttgatg atttctgtca tgatctggcg
     9421 catcagtgcc gaatcatcga cagataacac cctgattttg ctcatcgtta atccttactt
     9481 agcgcataca ccgtctgacc acgcagcgtg aagcggcgct caaggtggct aaagttttca
     9541 gagtgacccg caaacagcaa tccgtcgggt ttaaggagcg gaacaaagcg gcgcaaaatc
     9601 gcctgctggg tagtttgatc gaagtagatc atgacgttac gacagaagat cgcatcaaac
     9661 ggccccggca cggtgtactg tttcgccagt aaattcagcg gggcaaaatc aacatagttc
     9721 gccagctcct gacgcacgcg taccagccct tcatgcggtc ccgtccctcg catgaaatac
     9781 cgttgcagtt gctgcggcgt caggtttttc aactcttcat ggcgataaat accgcttctg
     9841 gctttttcca gcacttcggt gtcgatatcg ctggcaaaca ctttccagcg tccgggcgcg
     9901 gtgcccaatg tatcagccag cgtcatcgct atgctgtacg gctcttcgcc gcttgaagcc
     9961 gccgcgctcc atacgcgata ctcgccagaa cggcgacgtg cgtgatccgc gagcagagga
    10021 aaatggtgtg cctcacggaa aaatgccgtc agattcgtgg tcagcgaatt gataaacgcc
    10081 tgccactcac cgctgtgctg attagattcc agcaagttca ggtaatgacc gaaatccgcc
    10141 tgtcccagcg cacgcaaacg acgaaccagt cggttgtaaa ccatgtcgcg tttatggtca
    10201 gccagaacga tcccggcgcg ttgatagatc aattgactta tccgccgaaa atgcgcgtcg
    10261 gaaagcgcca ggcgctcggt catctgtaac aataaagacg tttgcccaca gggcagagat
    10321 gaagtcatag cgccttctca atcacttcag gataccactg gcgcaatttg taactgcgcc
    10381 gactcatgtc gtgctacttc atgttcttca agggtaaata ccgccacacg cgacgaaaga
    10441 tgctcgacct gattcagtcg ttgcaaatcg tcgcgaaatg cccaaaagac atgccgttac
    10501 tgccaatctg caagatcccg cagagaatca aaattaaaaa cagcgtggtc gaaattcgaa
    10561 tacgattaaa catcaacgct ccccatcagg cggcaatgac cgctttagta aatactcgtc
    10621 aaaatgtttc ccagtttgga tcttgttcag caattcgcag tcgtggctgt gccggtggtt
    10681 gctcactggc aggacgggat ggtgtttgcg gtttattggt gagtgggctg gctgccagac
    10741 ggaacgcgga aaccgcttgc gttaaacgac tcgcctgttc ttccagcgca gctgcggcgg
    10801 cagctgattc ctgcaccagc gatgcgttct gttgcgtgac gcgatccatt tccgaaaccg
    10861 ccaatgcgac ttgatcgatg ccacggctct gttcatccga tgctgatgca atctcgccca
    10921 taatgtcagt cacgcgagtg acagcattga cgatattgtt cattgtttcc ccggcgcttt
    10981 cgaccagcac cgaaccggta tcaacgcgcg agacggagtc ttcaatgagg gctttgatct
    11041 cttttgccgc ctgggcgctg cgactggcaa gattacgcac ttcgcccgcc accacggcaa
    11101 aaccacggcc ttgttcaccc gctcgcgcgg cttcaacagc ggcattcagc gcgaggatat
    11161 tagtctggaa ggcaatacca tcgataacgc tgataatgtc ggcaattttc ttcgaactat
    11221 cggcgatctc atgcatcgtt ttcactacgc catccaccac tttgccgccg tgctgggcgg
    11281 tgtcggaggc actttgcgcc agttgcgagg cctggcgggc gttatcggcg ttttgcttca
    11341 ctgtcgcggt gagctgctcc atgctggcgg cagtttcttc cagcgcggat gcctgctgtt
    11401 cagtacggga ggaaagatcg gtgttgcccg ccgcaatttc acgggtaccg gcatagatgg
    11461 catctgaacc ttcgcgcaca tgagtgacgg tgtcagtcaa agagcgttgc atatgtgaaa
    11521 cgctctgcgc caggtcgccc atttcactgc gcccgtcaat ggtcagggta ttcgccaggt
    11581 taccaccggc aatttcgcga atatgcgcaa taatttttgc cagcggtgta agcaacatac
    11641 ggcgaatgcc gtaccacgcc accagcagaa tcaataccac caccagcgcg ataaccgcca
    11701 gttgccactg ggcaaatcgg taatcatctg cgttgtcagt gacgatatcg cgatacagtt
    11761 tttcactgct gagggcgtac tgagcaaacg cttcgcccat tgcattttgc attccctggg
    11821 ttggctgagc gaaataagct ccagtattgc cataatcaag ataatcaatc agttcagtta
    11881 acgctgtgtg atagttttta tatttttcat caatattacg actggtagcg accatttcag
    11941 gtaacggtgc catgcttttg aattttttat aatgagtcgc ggcctgggcc aatgttttcc
    12001 tggcgctatc gagcaattca actttggcat tactttgctg attggatgaa tccatcatca
    12061 tccgcaccgc tgaacgactc aggttaatgc gcgtttgtag cattaaatcc catgttgacg
    12121 tcagttcgcc ctgctgttcc cgtaattgat tggaaaccac aaagctcttc tggctatggt
    12181 gaagggaaga aaaaaacaga ctgccggaaa taagctgtaa cagtgcgaat acccccagca
    12241 ccattaccaa cagcgtgact acgcggatac ggttaatcat aaggcacctt cctgaaaaca
    12301 agttgatctc gttatcggca aggagggggg aaactttatt gctgatgcca cccgccgcga
    12361 aattgaaata aaaaacccga tgcgcagatc atcgggttca tttcaattga ggaaatcggg
    12421 agaattacgc cacttctgac gccgcgctat ctaacagcgc catctcttcg ctgttcagca
    12481 gtttttcgat gttcaccaga atcaacatgc ggtcgcccag tgcgcccagt cctgtgagat
    12541 attcggttga aagcgtcacg gcaaattccg gtgccggacg aatttgctcc gccgtcaatg
    12601 aaagcacgtc tgagacgccg tcaaccacga tgccgaccac ccgctgtccg agattcagga
    12661 cgataactac cgtgttgtcg ttatagtcca catccacctg gctgaactta attcgtaagt
    12721 caacaatcgg cacaataacg ccgcgcagat tcgtgacgcc tttgataaac gctggcgtgt
    12781 tcgcaatccg tgtcacctga tcgtagccac ggatctcctg cactttcaga atatcaatac
    12841 cgtactcttc atcaccaagg gtaaatacca gaaattcctg gcctgacggc tcgctggcca
    12901 gctttgttac attcgtcata ccggtcatat tgttaccttt ttactcattc aggcggcggt
    12961 gttcgccata cgttgttcgc ggtttatcgc ctgcaaggcg gagacatcaa caatcagtgc
    13021 cacgctgccg tcgccaagaa tggtcgcagc agaaatgccg gggactttgc gatagttact
    13081 ttcaaggttt ttaaccacaa cctggtgttg accaattaat tgatccacca gcaaggcgta
    13141 gcggcgaccg ccactttgta agatcaccac aattccctgg gtggcttcgg ttttcgcgcc
    13201 cgcgacgttg aacactttcc acagttcgac gatgggcaga tattcacccc gcacttccag
    13261 cacccgctcg ccgcctgcca gtggatgcag atcggcttca cggggttgca gtgattccat
    13321 gacagcattc agcggcagaa tgaaaacttc atccgcaacg cgtacggaca tgccatcaag
    13381 aattgccagc gtcagcggca gtaaaatgcg gatcgtcgtg ccagtaccct gcttcgactg
    13441 gatctcgaca tgaccgccca tctcctggat attacgtttc acgacgtcca tgccgacgcc
    13501 gcgcccggag acgtcggtga cctgctccgc cgtggagaag ccaggggcaa atatcagcat
    13561 cgcaacttcg tcgtcgctca tgttttcgct gacggtcaga ccttgcgagg ccgcttttgc
    13621 cagaattcgt tcacggttta gccccgcccc atcgtcggtc acttcaatgc aaatgttgcc
    13681 gccctgatgt tcggcagaca gaattaaatt tccgacgctg tttttacctg cagcgagccg
    13741 tttttctggc agttcaatac cgtgatcgag gctattgcgt accaggtggg tcagcgggtc
    13801 gataatgcgt tcaatcaggc tcttgtcgag ttcagtagaa ctgcccacca gcgtcagttc
    13861 tacctgcttg ccgagttttc ccgccagatc acgcaccagc cggggatagc gactaaagac
    13921 atattccatc ggcatcatgc gaatcgacat cactgattcc tgcaaatcac gggcattacg
    13981 ttgtaactgc cccatgctgg taatcaaatc accatgatta accgggtcca gttcgctgga
    14041 acgctgggca agcatggact gggtgatgac cagctcaccg acgaggttaa ttaattgatc
    14101 aaccttttct accgctacac ggatgctggt ggattcactg ctgcgcgtcg ttttttcccg
    14161 ctccacgcga ccggttggcg cttgttcagc tgccagttta agcactggtg gggtggatat
    14221 ttttggcgag acgtctactg tttcaaaggt aatctgatcg gcttcaatca caaaacagag
    14281 taccgctgtg atgtcatctt cggcgatgtc gcccggtaat attgccgaga gagaatccgc
    14341 ccctttcacc acgtcagtta acgttgtcag atgccccagc tcttcttcca gcaggtcgac
    14401 ttccccggcc ttcaggcgcg aaaggataat tcgtcgcggt gactgactac gactctgctc
    14461 atcttgcggt tcacttttgg caaccacact taatcgggtc actgcggatg gcgtttcgtc
    14521 tttcgcttct aatgccagtt gacgcaaggc ctggcagata taatcgaagc tggcagcatc
    14581 cggctcttgc gactgtttat aagcgtcgag ctgttcttgc atgatgtcct tcgtttccaa
    14641 aaacagattg ataatgtcgg tgttgagttg catctcacct cgtctggctt cgtcgagcag
    14701 gttttccatc agatgcgtgg tttcctgcaa aacgctgaag ccaaaggttc ctgcccctcc
    14761 tttgatcgag tgggcagccc gaaagatggc attcaattgt tcggcatctg gcgcttccgg
    14821 ctgcaaaacc agcagatgct gctccatatc agccaacagt tcgtccgctt catcaaaaaa
    14881 tgtctgataa aaatcgctta tatccatgct cacgctgtca cctcggttcg gctgatggca
    14941 ttgtgggaac actgacctgt ggtgcaacct caggtttttc cagggcgctt actggctcat
    15001 tctggctttc ggcgttttca tgcaaaatgg cctgttcggc ttgtttgttc agtaccagca
    15061 ggctgatgcg acggttgacg gcatcatcag gtccgcgatc gcttaagcgc atcgttgccg
    15121 ccatgccgac gacacgtaac actttgccgc cattcaaccc accaaccatc agttcgcggc
    15181 gggatgcatt ggcacgatcg gcagaaagct cccagttgct gtaacctttc tcaccgctgg
    15241 cgtagggaaa atcatcggta tgacccgaga ggctaattcg gttgggaata ccgttcagta
    15301 cgggcgcgat ggcgcgcaga atgtcgcgca tatagggttc gacgtcggca ctgccagttc
    15361 taaacatcgg gcgattctgg ctatcgatga tctgaatacg tagaccttcc tggaccagat
    15421 cgattttgag atgtggacgt aacgcccgca gtttcggatc ggactcaatc aactggtcga
    15481 gatcgccacg caatttccgc aatcgacttt gctccatgcg ttttttcagc tcttcgatgt
    15541 tcggccgctt attcacttcc ccctggcttt gggtgtaatc atcaccaccg ccgggaattg
    15601 ggctttcact attggaaatg cgatcgccgc ccgtaaccgc tgtcgccagt ggagtccgga
    15661 agtactccgc aatctgaatc agctcttttg gactggagat agagatcagc cacatcacca
    15721 gaaaaaaggc catcattgcg gtcataaagt cggcataagc aatcttccac gatccatgtg
    15781 ctgctccgtg gcttttggct ttgcgtcgtt tgacgacaat aatcggatgc gcttgattct
    15841 tcatgcttcc tcggttgtcg tctgttgttg cggatttttc accgcacgca catgctcttc
    15901 cagctcaata aacgacggac gttcgctgga atagagcgtt ttgcgaccaa actcaacggc
    15961 gataggcggt gcgtaaccgt tcagattaga aagcagagtg actttgacgc actgcatcat
    16021 tttgctggtt tcggcgcttt tctgacgtaa aacactcgct aatggggaaa taaatccgta
    16081 agccaataaa ataccgagga aagtccccac catcgcatga gcgataagcg cccccagctc
    16141 ggcggcagga cgatcggctg aacctaacgc gtgaactacc cccattacag ccgcaacaat
    16201 accaaacgcc ggaagtgagt ctccgaccag cgccagactg ttcgccggga cttctgcctc
    16261 gctttcgtgc gtctcaatct cttcatccat cagagcttcg atttcgaagg tgttcatgtg
    16321 accgctgata atcaggcgca gataatcgac gataaattca agcatgacgc tatccgcaag
    16381 gatgcgtggg tagctggcga agatctcgct ctcacgggga ttttcaatat cgcgttccag
    16441 cgaaaacatc cccatctgcc gcgatttcgc catcaaccga taaagcagag ccagcagatc
    16501 catatacatt gctttggtgt atttggagcg acgaaacagc aacggcagcg ccttcagcgt
    16561 gcctttaatc gctttgccat tattgccgac gataaacgac ccaatccctg caccggcaat
    16621 aatcaccagt tcagcgggtt gatagagtgc tccaaggctt ccaccggtca tcaaataacc
    16681 gccgaaaact gtaccgagaa caaccaggta acctaataag ataagcacga catcatcctt
    16741 ccactgttga ccatgacagg atgttcagtc gtcaggcgtt aacacgcgat aggggcaaaa
    16801 aaaagcagcg gtacatcgtt accgctgctg gaatgtttcg ccacaccgta tcagttaaac
    16861 agcctgtact ctctgttcat ccagcagttg tgggataata tcggcaggat tctgggaaag
    16921 tttacgtctt tttactgccc gggatggcgg ctgacataag ctgcaggcaa agctgccaac
    16981 aggctggtga gcgtgggtaa taaaattgcc gccgcagcag ttgcagctgg aaagttgcag
    17041 taatccactt tcaacaaacc gcaccaatgt ccaggcacgg gttaatgcca gcagcggtcc
    17101 ttcttctgct tgcgggcact gttcaaggta taaacggtag gctttgatca ccgcatcgac
    17161 gccattacac aagccagttt tcagtaaaaa ctgccatgca ttacagaaca tcgaagcatg
    17221 aacgttttgt tcccaggtca taaaccagtc ggttgagaat ggcagcatgc ctttcggcgg
    17281 tgggcttccg cgcagctctt tatagagttt tatcagacgt ccgcgactta actgtgtttc
    17341 gctttccagc atctgcaaac gagcgcccag ggtgatcaat tccattgcca gctgaatatc
    17401 ccgcgcttcc tgaacaatgc ttttttcact catgatcagg cccttttctt gcgcagcgct
    17461 tcttcaggct gattaacatc attcagcaag cgtgttgaga gcatgatgcc ggtatgaatt
    17521 tgctggagat cgtcaacgcg ggaatcttgc gtcaactgag taatcgtctg gtggctgtca
    17581 aaacggaagt gacaaaccag ttgattggtt tctgccagct taaccatttg cggaagagtc
    17641 agtgccgcta acgttgtcgc catttcttca tttatgccga gacgaaacat agcggacgct
    17701 ttgtcctgaa caatcagacg ctgtgcaagt agtaaatatg acaagttgat gtcataaatg
    17761 tgtttcagca actcggaggt atgcattatt cccacccaga ataaccaact ttatttttat
    17821 gcggtctcac cgcaccccgt gatgtcgccg ggaagccccg gtaaaaaata attagcatta
    17881 gaatagttgc gataagctgc aataagcaga cccacctttt tggtttaata agtccttaca
    17941 aatagaaggg agtctttaca cttatctaag atttttccta aatcgacgca actgtactcg
    18001 tcactacacg cacatacaac ggaggggggc tgcgattttc aataatgtgt gatgcagatc
    18061 acacaaaacg ctcaattact
//
LOCUS       DAHHTG010000089        17900 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.089, whole genome shotgun sequence.
ACCESSION   DAHHTG010000089 DAHHTG010000000
VERSION     DAHHTG010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17900)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17900)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17900
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.089"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            54..1052
                     /gene="yiaK"
                     /locus_tag="NBI74_003118"
     CDS             54..1052
                     /gene="yiaK"
                     /locus_tag="NBI74_003118"
                     /EC_number="1.1.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydro-L-gulonate 2-dehydrogenase"
                     /protein_id="HCD1933406.1"
                     /translation="MKVTFEQLKAAFNRVLISRGVDSETADACAEMFARTTESGVYSH
                     GVNRFPRFIQQLENGDIIPDAQPKRITSLGAIEQWDAQRSIGNLTAKKMMDRAIELAA
                     DHGIGLVALRNANHWMRGGSYGWQAAEKGYIGICWTNSIAVMPPWGAKECRIGTNPLI
                     VAIPSTPITMVDMSMSMFSYGMLEVNRLAGRQLPVDGGFDDEGNLTKEPGVIEKNRRI
                     LPMGYWKGSGMSIVLDMIATLLSDGASVAEVTEDNSDEYGISQIFIAIEVDKLIDGPT
                     RDAKLQRIMDYVTSAERADENQAIRLPGHEFTTLLAENRRNGITVDDSVWAKIQAL"
     gene            1064..1528
                     /locus_tag="NBI74_003119"
     CDS             1064..1528
                     /locus_tag="NBI74_003119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HCD1933407.1"
                     /translation="MIFGHIAQPNPCRLPAAIEKALDFLRTTDFNALDPGVVEIDGKN
                     IYAQIIDLTTREAVENRPEVHRRYIDIQFLAWGEEKIGIAIDTGNNKVSESLLEQRDI
                     IFYHDSEHESFIEMIPGSYAIFFPQDVHRPACNKNATTAIRKIVVKVALAAL"
     gene            1551..2471
                     /locus_tag="NBI74_003120"
     CDS             1551..2471
                     /locus_tag="NBI74_003120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000856281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4862 family protein"
                     /protein_id="HCD1933408.1"
                     /translation="MKTNNAGYIIGAYPCAPSFHQRSEEEEKDFWRQLSETPDILGLE
                     QPCLENFHPLGDQWLLRHTPESWKFVVTAVMETMRRRSENSGFGLASSDEEQRQACVA
                     YYRHLFNKINALQANKVLALALQAAPLATNPNVMQATDAFARSLKEIASWDWPCKLVL
                     EHCDAMTSSSPRKGFLPLENVLEVITDYDISICINWARSAIEGRNTTLPLTHTQMAKQ
                     AGKLGALMFSGTTLNGAYGEWQDLHAPFAPFCAESLMTTDHVRELFNVAESSTLHFAG
                     IKLLEINATADVHHRIEILRNGIHSLNESR"
     gene            2585..3052
                     /gene="yiaM"
                     /locus_tag="NBI74_003121"
     CDS             2585..3052
                     /gene="yiaM"
                     /locus_tag="NBI74_003121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001553595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diketo-L-gulonate TRAP transporter small
                     permease YiaM"
                     /protein_id="HCD1933409.1"
                     /translation="MKRILEGILAIIIAILSCIIFINIILRYGFHTSILSIDELSRLL
                     FVWLTFIGAIVAYMDNSHVQVTFLVEKLSPANQQRVSLLTHTLILLLCLGLAWGAIEK
                     TAQDWSNLSPILGVPVGLMYAAAIPTSLIIALLELRHLYRQFTNTPSRNQQGA"
     gene            3055..4332
                     /gene="yiaN"
                     /locus_tag="NBI74_003122"
     CDS             3055..4332
                     /gene="yiaN"
                     /locus_tag="NBI74_003122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diketo-L-gulonate transporter large permease
                     YiaN"
                     /protein_id="HCD1933410.1"
                     /translation="MTILIFIVSLLGAIAIGVPIAWALLLCGISLMFWMDIFDVQILA
                     QTLVNGADSFSLLAIPFFVLAGEIMNAGGLSQRIVDLPMKLVGHRPGGLGYVGVLAAM
                     IMASLSGSAVADTAAVAALLVPMMRQANYPVNRAAGLIGSGGIIAAIIPPSIPLIIFG
                     VSSGLSISKLFMAGIAPGIMMGVTLMVTWWWQAKRLNLPCQPKASLREVWQSLVSGIW
                     ALFLPIIIIGGFRSGLFTPTEAGAVAAFYALFVSVVVYREMTFSTLYHVLINAAKTTS
                     VVMFLVASAAVSAWLITIAELPMMVSELLQPLVDSPRLLFIVIMLAIMVISTVMDLTP
                     TVLILTPVLMPLVKEAGIDPVYFGIMFIINCSISLITPPVGNVLNVVCGVAKLKFDDA
                     VKGVAPYVMVLFMLLALFIFIPELITAPLKWMS"
     gene            4345..5331
                     /gene="yiaO"
                     /locus_tag="NBI74_003123"
     CDS             4345..5331
                     /gene="yiaO"
                     /locus_tag="NBI74_003123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diketo-L-gulonate transporter
                     substrate-binding protein YiaO"
                     /protein_id="HCD1933411.1"
                     /translation="MKLRSVTYALLIAGVATFSTSSLAAQSLRFGYETSQTDSQHIAA
                     KKFNELLQEKTKGELKLKLFPDSTLGNAQAMISGVRGGTIDMEMSGSNNFTGLSPVIN
                     LLDVPFLFRDTAHAHKTLDGKVGDDLKVSLEGKGLKVLAYWENGWRDVTNSRQPVKTP
                     ADLKGLKIRTNNSPMNIAAFKVFGANPIPMPFAEVYTGLETRTIDAQEHPINVVWSAK
                     FFEVQKYLSLTHHAYSPLLVVINKAKFDGLTPEFQQALISSAQEAGSYQRKLVAEDQQ
                     KIIDGMKEAGVEVITDLDRKAFSDALGTQVRDMFVKDVPQGADLLKAVDEVQ"
     gene            5335..6831
                     /gene="lyxK"
                     /locus_tag="NBI74_003124"
     CDS             5335..6831
                     /gene="lyxK"
                     /locus_tag="NBI74_003124"
                     /EC_number="2.7.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-xylulokinase"
                     /protein_id="HCD1933412.1"
                     /translation="MTQYWLGLDCGGSWLKAGLYDREGREAGVQRLPLCALSPQPGWA
                     ERDMAELWQCCTAVIRSLLTHSGVSGEQIVGIGISAQGKGLFLLDKNDKPLGNAILSS
                     DRRAMEIVRRWQEDGIPEKLYPLTRQTLWTGHPVSLLRWLKEHEPERYAQIGCVMMTH
                     DYLRWCLTGVKGCEESNISESNLYNMSRGEYDPCLTDWLGIAEINHALPPVVGSAEIC
                     GEITAQTAVLTGLKAGTPVVGGLFDVVSTALCAGLEDEFTLNAVMGTWAVTSGITHGL
                     RDGEAHPYVYGRYVNDGQFIVHEASPTSSGNLEWFTAQWGEISFAEINQAVASLPKAG
                     GALFFLPFLYGSNAGLEMTSGFYGMQAIHTRAHLLQAIYEGVVFSHMTHLNRMRERFT
                     DVHTLRVTGGPAHSDVWMQMLADVSGLRIELPQVEETGCFGAALAARVGTRVYRDFSE
                     AQRDLQHPVRTLLPDMAAHQLYQQKYQRYQHLIAALQGYHARIKEHTL"
     gene            6828..7490
                     /gene="ulaD"
                     /locus_tag="NBI74_003125"
     CDS             6828..7490
                     /gene="ulaD"
                     /locus_tag="NBI74_003125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HCD1933413.1"
                     /translation="MSRPLLQLALDHSSLEAAQRDVTLLKDSVDIVEAGTILCLNEGL
                     GAVKALREQCPDKIIVADWKVADAGETLAQQAFGAGANWMTIICAAPLATVEKGHAMA
                     QNCGGEIQIELFGNWTLDDARDWHRIGVRQAIYHRGRDAQASGQQWGEADLARMKALS
                     DIGLELSITGGITPADLPLFKEIRVKAFIAGRSLAGAANPAQVAADFHAQIDSIWGGK
                     RA"
     gene            7483..8343
                     /locus_tag="NBI74_003126"
     CDS             7483..8343
                     /locus_tag="NBI74_003126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418039.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HCD1933414.1"
                     /translation="MRNHPLGIYEKALAKDLSWPERLVLAKSCGFDFVEMSVDETDER
                     LSRLDWSAAQRASLVTAMIETGVAIPSMCLSAHRRFPFGSRDDAVRQRAREIMSKAIR
                     LARDLGIRTIQLAGYDVYYEDHDEGTQQRFAEGLVWAVEQAAASQVMLAVEIMDTAFM
                     NSISKWKKWDEMLASPWFTVYPDVGNLSAWGNDVPAELKLGIDRIAAIHLKDTQPVTE
                     HSPGQFRDVPFGEGCVDFVGIFKTLHELNYRGSFLIEMWTEKAKEPVLEIIQARRWIE
                     ARMQEAGFIC"
     gene            8337..9032
                     /gene="araD"
                     /locus_tag="NBI74_003127"
     CDS             8337..9032
                     /gene="araD"
                     /locus_tag="NBI74_003127"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HCD1933415.1"
                     /translation="MLEQLKADVLAANLALPVHHLVTFTWGNVSAIDETRQWMVIKPS
                     GVEYEVMTADDMVVVEIASGKVVEGSKKPSSDTPTHLALYRRFAEIGGIVHTHSRHAT
                     IWSQAGLDLPAWGTTHADYFYGAIPCTRLMTAEEINGEYEYQTGEVIIKTFEERGLNP
                     AQIPAVLVHSHGPFAWGKNAADAVHNAVVLEECAYMGLFSRQLAPQLPAMQNELLDKH
                     YLRKHGANAYYGQ"
     gene            complement(9064..9879)
                     /locus_tag="NBI74_003128"
     CDS             complement(9064..9879)
                     /locus_tag="NBI74_003128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCD1933416.1"
                     /translation="MLELSMTLPIKVQNGGLFISRGVGRHPARRLESWEIIFVEKGRL
                     TIQEDERIFLVDAGESLLLWPNRQHVGVEDFPADLKFYWLHFEVKAPDNDPRWLTHLS
                     VPQQTKVADPQVLVALFRQFLSEQEKHQRTPALEFIVLLILQQLTRDARLDEQADGAG
                     TTLAWKAQKLIRTQYHRPLSTALLARELYCNADYLGRVYRRVFHLTLTEAIHRQRVRE
                     AEKLLISEARSLTEVAALCGFNDVGYFRQIFRKHTGLTPAAWKRRYSKEHINS"
     gene            10105..11505
                     /locus_tag="NBI74_003129"
     CDS             10105..11505
                     /locus_tag="NBI74_003129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCD1933417.1"
                     /translation="MTSTPITTADIAKGKIDDALSVREKIGYGLGDAGGTVITCLIMN
                     FLTFFYTDVFGLTPALVGTLFIALRVFDAISDPVMGVIADRTQSRWGRFRPWQLWIAI
                     PIGIIGILTFTVPDASMGVKIAWAFGTYLLLSVGYTAINVPYCALINTMTTRYNEVIS
                     CQSWRFVLCGVAGFLVSVGLPWMVDLFGQGNAARGYQLGVGVLCAIAVVMFLCCFFWV
                     CERVPLSTMGKFTLREHLAGLRNNDQLLLMLVMSFLLINVFNIRGGGYMYFITYVLQG
                     STGYTSLFFTMVTFASIIGSVIVSPLTRRFDTVKIYYYTNLLLAALAVLMWFLPSGPA
                     YQTLWLAVILGNGVILGFTLPLHFSLMAFADDYGEWKTRVRSSGMNFAFNLFFIKLAW
                     ASSAGIISLLFIFVAYQPGVENQTASSLGGIAAMETLLPALFHLLLAMAIRFCKLNNP
                     MMSRIASDLRQRHVQP"
     gene            11516..13489
                     /locus_tag="NBI74_003130"
     CDS             11516..13489
                     /locus_tag="NBI74_003130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001026880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 127 protein"
                     /protein_id="HCD1933418.1"
                     /translation="MNISEVDLHKLTVSDPFLGQYQQLVRDVVIPYQWDALNDRIPEA
                     EPSHAIENFRIAAGLQEGEFYGMVFQDSDVAKWLEAVAWSLCQKPDAELEKTADEVIE
                     LIASAQCEDGYLNTYFTVKAPEERWSNLAECHELYCAGHLIEAGVAFFQATGKRRLLE
                     VVCRLTDHIDSVFGPDESKLHGYPGHPEIELALMRLYEVTEEPRYLALTNYFVEQRGA
                     QPHYYDQEYEKRGQTSHWHTYGPAWMVKDKAYSQAHLPIAQQQTAIGHAVRFVYLMTG
                     VAHLARLSHDESKRQDCLRLWNNMAQRQLYITGGIGSQSSGEAFSSDYDLPNDTVYAE
                     SCASIGLMMFARRMLEMEGDSQYADVMERALYNTVLGGMALDGKHFFYVNPLEVHPKS
                     LKFNHIYDHVKPIRQRWFGCACCPPNIARVLTSIGHYLYTPREDALYINIYAGNSMEV
                     PVENGTLRLRVSGNYPWQEQVTIAVESPQPVRHTLALRLPDWCIQPQIILNGEEVEQD
                     IRKGYLHITREWQEGDTLNLTLPMPVRRVYGNPLVRHVAGKVAIQRGPLVYCLEQADN
                     GESLHNLWLPADAPFTTFEGNGLFRHKILIQAPGYRYEQSNPEQQPLWHYDSAPAKRQ
                     PQILTFIPWFSWANRGEGEMRIWVNEETSAIRR"
     gene            complement(13666..15204)
                     /gene="aldB"
                     /locus_tag="NBI74_003131"
     CDS             complement(13666..15204)
                     /gene="aldB"
                     /locus_tag="NBI74_003131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001551836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase AldB"
                     /protein_id="HCD1933419.1"
                     /translation="MTNNPPSAQIKPGEYGFPLKLKARYDNFIGGEWVAPADGEYYQN
                     LTPVTGQLLCEVASSGKRDIDLALDAAHKVKDKWAHTSVQDRAAILFKIADRMEQNLE
                     LLATAETWDNGKPIRETSAADVPLAIDHFRYFASCIRAQEGGISEVDSETVAYHFHEP
                     LGVVGQIIPWNFPLLMASWKMAPALAAGNCVVLKPARLTPLSVLLLMEIVGDLLPPGV
                     VNVVNGAGGEIGEYLATSKRIAKVAFTGSTEVGQQIMQYATQNIIPVTLELGGKSPNI
                     FFADVMDEEDAFFDKALEGFALFAFNQGEVCTCPSRALVQESIYERFMERAIRRVESI
                     RSGNPLDSVTQMGAQVSHGQLETILNYIDIGKKEGADVLTGGRRKLLEGELKDGYYLE
                     PTILFGQNNMRVFQEEIFGPVLAVTTFKTMEEALELANDTQYGLGAGVWSRNGNLAYK
                     MGRGIQAGRVWTNCYHAYPAHAAFGGYKQSGIGRETHKMMLEHYQQTKCLLVSYSDKP
                     LGLF"
     gene            complement(15312..16607)
                     /locus_tag="NBI74_003132"
     CDS             complement(15312..16607)
                     /locus_tag="NBI74_003132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HCD1933420.1"
                     /translation="MAIKSPPGLIPLSHLSGEELLAHLRFNRVTDEKGRYLPFDELQY
                     RIKKGENVDVAWTLTRLARNAAIQRINYCNEAGEQAGFNITPVIAEACELVDKRATAL
                     ALKDQTERLRGAGAELSQLRLEEPITSSQLEGANTTTLVARKMLETGRSPRTEDEHMI
                     AGNARLMAEIPHLLAEPLTPALIRQLHAIGMGGINDAKYRPGEFRETDDVVIADYDGN
                     IVHQPPAAALLPERLEKVCQWLNSHEGYIHPLVRACILHFMLAHEHPFRDGNGRTSRA
                     LFYWYMLKSGYDVFKYISISRLLHAAPVKYAASYQYTESDGMDLTYFLEYQAGVIKRA
                     LQNWQQHIDEITQRSAKLDSVLFSSGVLKRLNPRQVTLLNVMLANPGKEYTVAEISAS
                     LGVSDNTARADLRTIVKEGFAQEKRINNQQAVYVAHYPL"
     gene            complement(16737..17888)
                     /gene="yiaY"
                     /locus_tag="NBI74_003133"
     CDS             complement(16737..17888)
                     /gene="yiaY"
                     /locus_tag="NBI74_003133"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine dehydrogenase"
                     /protein_id="HCD1933421.1"
                     /translation="MAASTFFIPSVNVIGADSLNDAMNMMADYGFTRTLIVTDNMLTK
                     LGMAGDVQKALEERNIFSVIYDGTQPNPTTENVAAGLKLLKENNCDSVISLGGGSPHD
                     CAKGIALVAANGGDIRDYEGVDRSAKPQLPMIAINTTAGTASEMTRFCIITDEARHIK
                     MAIVDKHVTPLLSVNDSSLMIGMPKSLTAATGMDALTHAIEAYVSIAATPITDACALK
                     AVTMIAENLPLAVEDGSNAKAREAMAYAQFLAGMAFNNASLGYVHAMAHQLGGFYNLP
                     HGVCNAVLLPHVQVFNSKVAAARLRDCAAAMGVNVTGKNDAEGAEACINAIRELAKKV
                     DIPAGLRDLNVKEEDFAVLATNALKDACGFTNPIQATHEEIVAIYRAAM"
BASE COUNT         4216 a         4730 c         4630 g         4324 t
ORIGIN      
        1 gtatattgaa attttaagcc aaaaaagcga tcaaaaaaac aaggaagcct gggatgaaag
       61 tgacatttga gcagttaaaa gcagccttta atcgggtctt aatttcacgc ggcgttgaca
      121 gcgaaacggc tgacgcctgt gcagagatgt tcgcccgcac caccgaatcc ggcgtttatt
      181 ctcacggcgt taatcgtttc cctcgtttca ttcaacaact ggaaaacggc gatatcattc
      241 ctgatgccca acccaaacgt ataaccagcc tcggcgcaat tgaacagtgg gacgcccagc
      301 gttcgatcgg taacctgaca gcgaaaaaga tgatggatcg cgccattgaa ctggctgccg
      361 atcacggtat tggtctggtg gcactacgta atgccaacca ctggatgcgc ggcggcagct
      421 acggctggca ggcggcggaa aaaggctaca ttggcatttg ctggaccaac tccatcgccg
      481 taatgccacc gtggggcgca aaagagtgtc gcatcggcac caacccgctg atcgtcgcca
      541 ttccttccac gccgatcacc atggtcgata tgtcgatgtc gatgttctct tacggcatgt
      601 tagaagttaa tcgcctggct ggtcgtcagc tcccggtaga tggtggcttt gatgatgagg
      661 gcaatttgac caaagaacct ggcgttatcg agaaaaatcg ccgcattttg ccgatgggct
      721 actggaaagg ttcgggcatg tcgattgtgc tggatatgat cgctaccctc ctttctgacg
      781 gcgcatcggt tgccgaagtc accgaggaca acagcgacga atacggcatt tcacaaattt
      841 ttattgccat tgaagtggac aagcttatcg acggtcccac ccgcgatgcc aaactgcaac
      901 gcattatgga ttacgttacc agtgccgagc gtgctgacga aaatcaggcc attcgcttac
      961 ccggccacga atttactacc ctgctggcag aaaaccgccg taacggtatt accgttgatg
     1021 acagcgtgtg ggcaaaaatc caggcgttat aaggagataa gccatgattt ttggacatat
     1081 cgcgcagcct aatccgtgcc gtttgcccgc cgccattgaa aaggcgctcg attttctgcg
     1141 caccaccgac ttcaacgccc tggatccggg cgttgtcgaa atcgacggca agaatattta
     1201 tgcgcaaatt atcgatttaa ccacacgcga agcggtggaa aaccgtccgg aagtgcatcg
     1261 tcggtatatc gatattcagt ttctggcgtg gggtgaagag aaaattggca ttgccattga
     1321 tacgggaaac aataaagtca gcgaatcact attagagcag cgcgatatta ttttttatca
     1381 cgatagtgaa catgaatcat ttatagaaat gataccgggt agttacgcta ttttcttccc
     1441 gcaggatgta catcgcccgg catgtaataa aaatgccact acggcaatac gcaagatcgt
     1501 ggttaaagtg gccctcgctg ctttataaaa aatgattact ggagcacata atgaaaacga
     1561 ataatgccgg ttatattatc ggcgcgtatc cctgtgcacc ttcttttcac caaagaagtg
     1621 aagaggaaga gaaggatttc tggcgacaat tatctgagac tccagacatt ttgggtctgg
     1681 aacaaccttg cctggaaaat tttcaccctc ttggcgatca atggttactc cgtcatacgc
     1741 cagagagctg gaaatttgtc gtcaccgcag tgatggaaac catgcgtcgc cgcagcgaaa
     1801 acagcgggtt tggtctcgcc tccagtgatg aagaacagcg ccaggcctgc gtggcgtact
     1861 atcgccacct gttcaataaa atcaacgcat tgcaggctaa taaagtgctc gcactggcgc
     1921 tccaggctgc accgttagca acgaatccta atgttatgca agcgacggac gcttttgccc
     1981 gctcattgaa agaaatcgcc agttgggact ggccgtgcaa gctggtgctg gaacactgcg
     2041 acgccatgac atcttcttcc ccccgaaaag gctttttgcc tttagaaaat gtgctggaag
     2101 ttattaccga ttatgacatc agcatctgta tcaactgggc gcgctctgcc attgaagggc
     2161 gcaatacaac gctgccgctg acccacaccc aaatggcgaa acaagccgga aaactcggcg
     2221 cgttgatgtt ctctggtacg acgctaaacg gggcatacgg cgaatggcag gatttacacg
     2281 caccgtttgc accattctgc gcagaaagcc tgatgaccac agatcatgtt cgcgagttat
     2341 ttaatgtagc agaatcgtca acattgcatt ttgctggcat caagctactg gaaataaatg
     2401 cgacggctga tgtgcaccat cgcatcgaaa tattacgtaa cggcattcac tctctgaatg
     2461 aatctcgtta aatagatccg cttatatatc actttgaata tctaattagc cgttttctga
     2521 taattattac gaatattaac aacattagcg cataaaaaat attgcataac cagaaggact
     2581 cgttatgaaa cggatactcg aaggcattct tgcgatcatc atcgcaatac tgtcttgtat
     2641 tatttttatt aatatcattc ttcgatatgg attccacaca agtatattat cgattgatga
     2701 attatcccga cttttatttg tctggttaac ctttattggc gcaatcgttg cttatatgga
     2761 caacagccat gtgcaggtta cttttttagt cgaaaaactc tcaccggcta atcaacaacg
     2821 tgtttcgctg ctaacccata cgctcatttt attgctgtgt ctggggctgg cctggggcgc
     2881 tatagaaaaa acagcgcaag actggagcaa cttatcgccc attcttggcg tccccgttgg
     2941 cttaatgtac gccgccgcta tccccaccag tctgattatc gcattgctgg aattgcgtca
     3001 cttataccgg cagttcacaa atacccccag ccgaaaccaa caaggagcct gacgatgaca
     3061 atccttatat ttattgtcag tttgttaggg gctatcgcaa ttggtgttcc catcgcctgg
     3121 gcattattgt tgtgcggcat ctcattgatg ttctggatgg atatctttga tgtgcaaatt
     3181 ctggcacaaa cgttggtaaa tggcgcagac agcttctccc tgttagccat ccccttcttt
     3241 gtactggcgg gtgagatcat gaacgcgggc ggcctgtcgc aacgtatcgt cgacttgcca
     3301 atgaaactgg taggccacag gccaggcggt ctgggttacg tgggcgtgct ggcagcgatg
     3361 atcatggcaa gcctgtccgg ttctgcggtt gctgataccg cagctgtcgc ggcgctgctg
     3421 gtgccgatga tgcgccaggc caactatccg gtaaaccgcg ccgccgggct tatcggttcc
     3481 ggcgggatta ttgcggccat tatcccacct tccataccac tcattatctt cggcgtctcc
     3541 agcggactct ccatcagtaa gctgtttatg gcgggcatcg caccgggcat catgatgggc
     3601 gtcacgctga tggttacctg gtggtggcag gcaaaacgac tgaatttacc ttgtcagccg
     3661 aaagcctcac tgcgagaagt ctggcaatcc ttagtttccg gtatttgggc gctcttttta
     3721 ccgataatta ttattggcgg tttccgttcg gggttattta caccgaccga agcaggcgcg
     3781 gttgccgcct tctacgctct gttcgtctcg gtggtggtct atcgcgagat gaccttctcc
     3841 acgctttatc atgtgctgat taacgcggca aaaaccacat ccgtggtgat gttcctggtg
     3901 gcctctgctg cggtatctgc atggcttatt acgatcgccg aattacccat gatggtatcg
     3961 gagctattac aaccgttggt tgattcgcct cgcttattat tcatcgtcat tatgctcgct
     4021 attatggtga tcagtacggt catggactta acgcctaccg tattaattct tacaccagta
     4081 ttaatgccat tagtgaaaga agcgggaata gatccggtct atttcggcat catgtttatt
     4141 atcaactgtt caatatcact gattacgcct ccagtcggta atgttcttaa cgtggtatgc
     4201 ggcgtggcga aacttaaatt tgacgatgcg gtaaaaggcg ttgcgcctta cgtcatggtt
     4261 ttattcatgt tgttagcatt atttattttt ataccggagc tcattaccgc tcctcttaaa
     4321 tggatgagtt aaaaggaaat cataatgaaa ttacgttctg taacctacgc tttattaatt
     4381 gccggagtgg cgacgttcag cacatcttct ctggcggcac aatcattacg tttcggttat
     4441 gaaacctcac aaaccgattc acaacatatt gcggcgaaaa aatttaatga attactgcag
     4501 gaaaaaacca aaggcgaact gaaattaaaa ctgttcccgg atagcaccct tggtaatgcg
     4561 caggcgatga tcagcggcgt gcgtggcggc actattgata tggagatgtc tggctctaac
     4621 aactttacgg gactctcgcc cgtcatcaac ctgctcgatg tcccgttcct gttccgcgat
     4681 accgctcacg cgcataaaac gctcgacggc aaagtcggtg atgacctgaa agtctccctt
     4741 gaaggtaaag ggctgaaagt gctggcttac tgggaaaacg gctggcgcga tgtcaccaac
     4801 tcacgccagc cagttaaaac acctgccgac ctgaaaggac taaaaatccg caccaacaac
     4861 agcccgatga atatcgccgc atttaaagtt tttggcgcta acccgatccc gatgccgttt
     4921 gccgaagtct ataccgggct ggaaacccgc actatcgacg ctcaggaaca cccgatcaac
     4981 gtcgtctggt cagcaaaatt tttcgaagtg cagaagtacc tttctctgac ccatcacgcc
     5041 tattccccac ttctggtggt gatcaacaaa gcgaagtttg atggcttaac cccggagttc
     5101 cagcaggcac tgatctcatc tgcgcaggaa gcgggtagct atcagcgcaa actggtcgcc
     5161 gaagatcagc aaaaaatcat cgacggtatg aaagaagcgg gcgtggaagt catcaccgat
     5221 ctcgaccgca aagcctttag cgatgcgctg ggtacccagg tccgtgacat gtttgttaaa
     5281 gatgttccgc agggtgctga tctgctgaaa gccgtggatg aggtgcaata aaccatgacg
     5341 caatactggc tggggttaga ttgtggcggt agctggctga aagccgggct gtatgaccga
     5401 gaaggccggg aggcgggcgt gcagcgcctg ccgctgtgcg cattaagtcc acagccaggc
     5461 tgggcagagc gcgacatggc agaactgtgg caatgttgca ctgctgtcat tcgctccctg
     5521 cttacccatt ctggcgtcag cggggaacaa attgtcggta tcggcatctc cgcgcaggga
     5581 aagggcttat ttctacttga taaaaacgac aaaccgctcg gtaacgccat tttgtcctcg
     5641 gatcgccggg cgatggaaat cgttcgtcgc tggcaggaag atggcatccc ggaaaaactc
     5701 tatccgctga cccgacaaac cttatggacc gggcatccgg tgtcgctgtt acgctggctg
     5761 aaagagcacg aaccagaacg ctacgcgcaa attggctgcg tgatgatgac gcacgactac
     5821 ctgcgctggt gtttaaccgg cgtcaaaggt tgcgaagaga gcaatatttc tgagtccaat
     5881 ctctacaaca tgagtcgcgg ggaatatgac ccgtgcctaa ccgactggct ggggatcgct
     5941 gaaatcaatc acgccctgcc gcctgttgtc ggatctgccg aaatctgcgg ggaaatcacc
     6001 gctcagacag ccgtactgac cggtctgaaa gcgggtaccc ccgttgtcgg cggcctgttt
     6061 gatgtggttt ccaccgcact ctgcgccggg ctcgaagacg aattcaccct caatgcggtg
     6121 atggggacct gggcggtgac cagcggcata acccacggtt tacgtgacgg tgaagcgcat
     6181 ccgtatgtct atggtcgcta cgttaacgat ggtcaattta tcgttcacga agccagcccc
     6241 acctcttccg gcaatctcga atggtttacc gcacagtggg gagaaatctc atttgctgag
     6301 attaatcagg ccgtagccag tttgccgaaa gccggtggcg ctctcttttt cctgccgttc
     6361 ctgtacggca gcaacgccgg actcgaaatg accagcggtt tctacgggat gcaggccatt
     6421 cacacccgtg cacacctctt acaggccatc tatgaagggg tggtgttcag ccatatgacc
     6481 catctcaacc ggatgcgcga acgttttact gatgtgcaca ccctgcgcgt cactggcggc
     6541 ccggcgcact ccgatgtctg gatgcaaatg ctggctgacg tcagcggtct gcgtatcgag
     6601 ctgccgcagg tggaagaaac cggctgcttt ggtgcggcac ttgccgcccg cgtcggtacc
     6661 agggtttatc gcgatttcag cgaagcccaa cgtgacttac agcacccggt acgcaccctg
     6721 ctgccggata tggccgccca tcagctttac caacaaaaat accaacgcta tcaacatctc
     6781 attgccgcac ttcagggtta tcacgcccgc atcaaggagc acacattatg agtcgaccac
     6841 ttctgcaact ggccctcgac cactcatcac ttgaagccgc gcagcgcgac gtgacgctgt
     6901 taaaagacag cgtcgatatt gtcgaagcag gcaccattct ctgtttaaac gaagggcttg
     6961 gcgcagtgaa agccttgcgc gaacagtgcc cggacaaaat catcgttgcc gactggaagg
     7021 tcgccgacgc gggtgaaacg ctcgcgcaac aggcatttgg cgcaggcgct aactggatga
     7081 ccatcatctg cgccgcgccg ctcgccacgg tggaaaaagg ccacgcgatg gcacagaact
     7141 gcggcggtga aattcagatc gagctattcg gcaactggac gctggacgat gcccgtgact
     7201 ggcatcgtat tggtgtacga caggcgattt atcatcgtgg tcgtgacgcg caggccagcg
     7261 ggcaacagtg gggcgaggcc gatctggcac gcatgaaggc gctttccgat atcggccttg
     7321 agctttccat taccggcggg attacccctg ctgacctgcc gctgtttaaa gagatccgcg
     7381 tgaaagcgtt tattgccgga cggtcgctgg caggcgcagc aaatccggcg caagtggccg
     7441 ctgattttca tgcgcaaatc gactccatct ggggaggcaa gcgtgcgtaa tcatccgtta
     7501 ggcatttatg aaaaagcgct ggcgaaagat ctctcctggc cggagcggct ggtgctggca
     7561 aaaagctgtg gctttgattt tgtcgaaatg tcggtggatg aaaccgacga acggttgtca
     7621 cgtcttgact ggagcgccgc acaacgtgct tcgctggtca ctgcgatgat cgaaacgggt
     7681 gttgctattc cgtcaatgtg cctgtccgca catcgtcgtt ttccttttgg tagccgggac
     7741 gacgccgtgc gccagcgggc gcgggagatt atgagtaaag cgattcgcct ggcgcgcgat
     7801 ctcggcattc gtactattca actggcaggt tacgacgtct attacgaaga ccatgacgaa
     7861 ggcacccagc aacggtttgc tgaagggctg gtgtgggcgg ttgaacaggc agcggcatca
     7921 caagtcatgc tggcggtgga gattatggac accgcgttta tgaactctat cagcaaatgg
     7981 aaaaagtggg acgaaatgct tgcgtcaccg tggttcaccg tttacccgga cgtcggcaac
     8041 ctcagcgcct ggggcaatga tgttcccgcc gaactgaaac tgggcatcga ccgtatcgcc
     8101 gccatccacc tgaaagacac tcaacccgtt accgaacata gccccggaca gttccgcgat
     8161 gtgccgtttg gcgaaggctg tgtcgatttt gttggcatct tcaaaacgct gcatgagctg
     8221 aactatcgcg gttctttcct gattgagatg tggaccgaaa aagccaaaga gccggtgctg
     8281 gagattattc aggcgcggcg ctggattgaa gcgcgtatgc aggaggccgg atttatatgt
     8341 tagagcaact gaaagctgac gtgctggcgg caaacttggc gcttcccgtt catcatctgg
     8401 tgacgttcac ctggggcaat gtcagcgcga tagacgaaac gcggcaatgg atggtgatta
     8461 agccttccgg cgttgagtat gaggtaatga ccgccgacga tatggtggtg gttgagatag
     8521 ccagcggcaa ggtggtggaa ggcagcaaaa agccctcttc cgacacgcca acgcatctgg
     8581 cgctttaccg tcgctttgcg gagattggcg gcatagtgca tacccactcg cgccacgcca
     8641 ccatctggtc gcaggccgga cttgatctcc ccgcctgggg cactactcac gccgattatt
     8701 tttacggagc catcccctgc acgcgcctga tgaccgcaga agagatcaac ggtgagtatg
     8761 aataccagac cggcgaagtg atcattaaaa ccttcgaaga gcgtgggttg aacccggcac
     8821 aaatcccggc ggtactggtg cattctcacg gcccgttcgc ctggggaaaa aatgccgccg
     8881 atgcggtgca taacgccgtg gtactggaag agtgcgccta tatgggtctg ttctcgcgcc
     8941 agcttgcgcc acagctccct gcgatgcaaa acgaactgct ggataagcac tacctgcgta
     9001 agcatggggc caatgcctat tacgggcagt aatccctcac gccggggctt catcgccccg
     9061 gcactacgaa ttgatatgtt ccttgctgta acgccgcttc cacgctgctg gcgttaagcc
     9121 ggtgtgtttg cgaaaaatct gccggaaata gccgacgtca ttaaacccgc acaacgccgc
     9181 cacttccgtg agcgatcgcg cttcgctaat taacagcttt tcggcttcgc gcacccgctg
     9241 gcggtggatt gcttctgtta acgtcagatg aaacacccgc cgatagacgc gcccaagata
     9301 atcggcattg cagtacagct cgcgggctaa aagcgccgtg gaaagcgggc ggtgatattg
     9361 cgtgcgaatc agtttctgcg ctttccaggc aagggtggtg cctgcaccat cggcctgttc
     9421 atcaagacgc gcgtcacggg tgagctgctg caaaatcagc agcacaataa actccagcgc
     9481 cggagtgcgt tgatgctttt cctgttcgct taagaactgg cgaaacaagg ccaccagcac
     9541 ctgcggatcg gcaaccttag tttgttgcgg cacgctaaga tgagtgagcc agcgcggatc
     9601 gttgtccggc gctttaacct caaaatgcag ccagtaaaat ttgagatcgg cgggaaaatc
     9661 ctccacccca acatgctgtc ggttcggcca gagtaataaa ctctctcccg catcgaccag
     9721 aaatatccgt tcatcttcct gaatcgttaa tcgacctttt tcgacgaaga taatttccca
     9781 tgactccagc cgccttgcag gatgcctccc tacgccacgg gaaataaata atccaccatt
     9841 ttgcacttta atcggtaacg tcatggataa ttcgagcatt gatatgtaat ttcccgctta
     9901 ttcattaatg attaaacaca ttaccttttg attattaatc aaaaacatta atttcacctg
     9961 atccatttca cacttttaaa ttcaggtcgg aatcgtcacc tttttttact cttttctcgc
    10021 cttgttgata ttcccgttca cggcagaatt aatcaggtca ttgcaataaa actaaaaatt
    10081 gctccctatt cacgaggaat atctatgacc tctacaccga ttaccaccgc tgatatcgct
    10141 aaaggtaaaa ttgacgatgc gttatctgta cgggaaaaaa taggctacgg cctgggtgac
    10201 gcaggcggca ccgtaataac ttgcctgatc atgaactttc tcaccttttt ctacaccgac
    10261 gtttttggct taactccggc gctggttggc acgctgttta ttgcgctacg cgtgtttgat
    10321 gccatctccg acccggtgat gggcgtcatt gccgaccgga cgcaaagtcg ctgggggcgc
    10381 tttcgtccgt ggcagttatg gattgccatt cccatcggca ttatcggcat cctgacattc
    10441 accgtgccag atgccagcat gggagtaaaa atcgcctggg cgttcggtac ttacctgctc
    10501 ctttcagtcg gttatacagc catcaacgta ccgtactgcg cgctgatcaa caccatgacc
    10561 acccgctaca atgaagtgat ctcctgccag tcctggcgat tcgttctctg cggcgtagcg
    10621 ggatttctgg tttcggtagg cttaccgtgg atggtagatc tcttcggtca gggcaacgct
    10681 gcacgcggct atcaacttgg cgtcggcgta ttgtgcgcca ttgccgtggt gatgttcctg
    10741 tgctgtttct tctgggtttg tgaacgggtg ccactctcca caatggggaa atttaccctg
    10801 cgcgaacatc ttgccgggct gcggaacaac gaccaactgc tgctgatgct ggtcatgtct
    10861 ttcctgctga ttaacgtctt taatattcgc ggcggtgggt atatgtactt cattacctac
    10921 gtcttacaag gcagtacggg ctacacgtcg ctgttcttca ccatggtcac cttcgcctcc
    10981 attatcggct cggtgattgt cagcccatta acgcggcgtt tcgataccgt caaaatttac
    11041 tactacacca acctgctcct cgctgcgctg gcggtgttga tgtggttcct gccttctggc
    11101 ccggcttatc aaacgctgtg gctggcggtg atcctcggta atggcgtgat tcttggcttc
    11161 acattgccat tgcacttctc attgatggcc tttgccgatg actacggcga gtggaaaacc
    11221 cgcgtacgtt cttccggcat gaacttcgcc ttcaatctgt ttttcatcaa gctggcatgg
    11281 gcctccagcg ccgggatcat cagcctgctg tttatttttg tcgcctacca gcctggcgtg
    11341 gaaaaccaga ccgccagttc gcttggcggg atcgcggcga tggaaacatt gctgcctgcg
    11401 ctgttccacc tgctgctggc gatggcgatc cgcttttgca aactcaataa ccctatgatg
    11461 tcacgcattg ctagcgacct gcgtcagcgt catgtacagc cttaaggagc acaagatgaa
    11521 catttcggaa gtcgatctgc ataaactgac ggtcagcgat ccgttcctcg gtcagtacca
    11581 acaactggtc cgcgacgtgg tgattcctta tcagtgggat gccttgaacg atcgtatccc
    11641 agaagcggaa cccagccatg cgattgaaaa ctttcgcatt gccgccggac ttcaggaggg
    11701 tgaattttac gggatggtgt ttcaggacag cgacgtcgcc aaatggctag aagcggtagc
    11761 ctggtcgctg tgccagaagc cggacgccga actggaaaaa accgccgacg aggtgattga
    11821 actgatcgct tccgcccagt gcgaagatgg ctatctcaat acttacttta cggtaaaagc
    11881 gcccgaagaa cgctggagca atctggcgga gtgtcatgaa ctttactgcg ccggtcatct
    11941 gattgaagcc ggagtcgcct tcttccaggc tacgggcaag cggcgcttgc tggaagtggt
    12001 ttgccgtctg accgatcata tcgacagcgt atttggtcca gatgaaagta agttacacgg
    12061 ttatcctggt cacccggaaa ttgaactggc actaatgcgc ctgtatgaag tgaccgaaga
    12121 gccgcgctac ctggcgctga cgaactattt tgtcgaacag cgtggtgcgc aaccgcacta
    12181 ttacgaccag gaatatgaaa agcgcgggca gacctcgcac tggcacacct acggcccggc
    12241 gtggatggtg aaagacaaag cctacagcca ggcacatttg cccatcgcac agcagcaaac
    12301 cgccattggt cacgcggtac gttttgtcta cctgatgacc ggcgtcgcgc atctcgcgcg
    12361 tttaagtcac gatgaaagca agcgtcagga ctgcctgcgg ctgtggaaca atatggccca
    12421 gcgtcagtta tatattaccg gcggcattgg ctcacagagc agcggtgaag cgttcagcag
    12481 cgattacgat ctgccgaatg acacggtata cgctgaaagt tgcgcctcca tcggcctgat
    12541 gatgtttgcc cgacgaatgc tggaaatgga aggcgacagt caatatgccg atgtgatgga
    12601 gcgcgcactg tacaacaccg tgctcggcgg catggcgctg gatggcaaac atttcttcta
    12661 tgtgaatccg ctggaagtac atccaaaatc gctgaaattc aaccatatct acgatcacgt
    12721 taagccgatc cgccagcgtt ggtttggctg cgcttgttgt ccgccaaata tcgcccgcgt
    12781 gctaacctcg attggtcatt atctctacac gccgcgtgaa gatgcgttgt atatcaacat
    12841 ctacgcagga aacagcatgg aggtgccggt agaaaatggc acgctgcgcc tgcgggttag
    12901 cggaaactat ccgtggcagg aacaggtgac gattgcggtt gaatcacccc agccggtgcg
    12961 tcatacgctg gctttacgtc taccggactg gtgcatacag ccgcagatca tattgaatgg
    13021 ggaagaggtc gagcaggata ttcgtaaagg gtatttgcac attacccgtg aatggcagga
    13081 gggcgacacg ctgaatctga ctttgccaat gccggtacgt cgcgtttacg gtaacccgct
    13141 ggtgcgtcac gtcgccggaa aagtggcgat tcagcgcggc ccgctggtgt attgtctgga
    13201 acaggccgac aacggcgagt cactgcataa tctgtggctg cccgccgatg cgccatttac
    13261 gacatttgaa ggtaatggat tgttccgcca taagatctta atccaggcac cgggttaccg
    13321 gtatgaacag agcaatccag agcagcaacc gctgtggcat tacgacagcg cgccagccaa
    13381 acgccagccg caaattctga cgtttatccc gtggtttagc tgggccaacc ggggtgaagg
    13441 cgaaatgcgg atctgggtga atgaggaaac cagtgccatc cgtaggtagg ataaggcgct
    13501 cgcgccgcat ccaacgcatg gtgcacattg cctgatgcgg cgtgaacgct ttatccgacc
    13561 tacaaaagca tgcaaattca atatattgca tgagctatgt aggcatgata agacgcgaaa
    13621 gcgtcgcatc aggcaatgaa tacccgatgc gaccagcttc ttacatcaga acagccccaa
    13681 cggtttatcc gagtagctca ccagcaggca cttggtttgc tggtaatgct caagcatcat
    13741 tttgtgggtt tcgcggccaa tgcctgattg tttgtagccg ccaaacgccg catgtgccgg
    13801 gtaagcgtga taacagttgg tccacacgcg cccggcctga atgccgcgcc ccatcttata
    13861 ggccagatta ccgttgcggc tccagacgcc cgcgcccaaa ccatattgcg tatcgttcgc
    13921 cagctccagc gcttcttcca tcgttttgaa ggtggttacc gccagcaccg ggccaaaaat
    13981 ctcctcctga aacacgcgca tattgttctg accaaacaga atcgtcggtt cgaggtagta
    14041 gccgtctttc agttcgcctt ccagcagctt gcgccgcccg ccggtgagca catcagcgcc
    14101 ctctttttta ccgatatcaa tgtagttgag gatggtttcc agttgcccgt gagaaacctg
    14161 cgcgcccatt tgcgtcacgc tgtcgagcgg gttaccgctg cgaatgcttt cgacacggcg
    14221 gatagcgcgt tccataaagc gttcgtagat agactcctgc actaaagcac ggcttggaca
    14281 ggtgcaaact tcgccctggt taaaggcaaa cagtgcaaag ccttccagcg ctttatcgaa
    14341 aaaggcatct tcttcatcca tcacatcagc aaagaagata tttggcgatt tgccgcccag
    14401 ctccagcgtc accggaataa tgttttgcgt ggcgtattgc ataatttgtt ggcccacttc
    14461 cgttgagccg gtaaacgcca ctttggcgat gcgtttcgag gtcgccagat attcgccaat
    14521 ttccccacct gcgccgttga ccacgttcac cacccctggc ggcagtaaat caccgacgat
    14581 ttccattagc agcagtacag aaagcggagt aagacgtgcg ggtttcagca ccacacagtt
    14641 gccagccgcc agcgcgggag ccattttcca gctcgccatc agcagcggga agttccacgg
    14701 gataatctgc cccaccacgc ctaacggttc atggaaatga taggccacgg tttcgctatc
    14761 cacttcactg atcccacctt cctgcgcccg aatacacgag gcgaaatagc gaaaatgatc
    14821 aatcgccagc ggcacgtcgg cagcactggt ttcgcgaatg ggtttgccgt tatcccaggt
    14881 ttctgccgtc gctaaaagtt caaggttttg ttccattcga tcggcaatct taaacagaat
    14941 cgccgcgcga tcctgcaccg aggtgtgcgc ccatttatct ttcactttat gcgcagcatc
    15001 cagcgccaga tcgatgtctc gtttgcccga agacgccact tcgcacagta gctgcccggt
    15061 caccggcgtc aggttctggt aatactcgcc atcggcaggc gctacccatt cgccaccgat
    15121 aaagttgtcg tagcgggctt ttaatttgag ggggaaacca tactcgccgg gcttaatctg
    15181 tgctgaaggg ggattattgg tcatgatcgt ctccttgcgg tgtgaggtat aacaagggta
    15241 gacgtgactg gcgaaatctt cgccagtcgg taacagcttt acgacggcta tcacgaattt
    15301 acgggcaaaa gctacagcgg gtagtgtgcg acgtacactg cttgctggtt attaattctt
    15361 ttctcttgcg cgaatccttc tttaacaatt gtccgtaaat cagctcgagc cgtattatca
    15421 gagacgccca agctcgcgct aatttcagca actgtgtact ctttccccgg attggcgagc
    15481 atcacattca ggagcgttac ctggcgcgga ttcaggcgtt taagcacacc agagctaaac
    15541 agcacactat cgagttttgc gcttcgttgt gtaatttcat cgatatgctg ctgccagttt
    15601 tgcagagcac gtttaatgac acccgcctga tattcaagga aataagtcag atccatacca
    15661 tctgattcag tgtactggta gctggcggca tatttgaccg gtgcagcatg aagcaaacgg
    15721 ctgatggaaa tatatttaaa gacatcgtag cccgatttca gcatatacca gtaaaacagc
    15781 gcccgactgg tgcgcccgtt gccatcccga aacggatgtt catgggccag cataaaatgc
    15841 agaatgcagg cacggaccaa cggatgaata tacccttcat ggctgtttaa ccactggcaa
    15901 actttttcca gccgttccgg taacaatgcc gcagcgggcg gttgatgcac tatattgcca
    15961 tcatagtcgg cgatcacaac atcatctgtt tcacgaaatt ctcccggacg atatttcgcg
    16021 tcattgattc cacccatacc aatggcatgt aactggcgaa taagcgcagg tgtcagcggt
    16081 tctgcgagta agtgcggaat ttccgccatc agccgggcat ttcccgcaat catatgctcg
    16141 tcttcagtgc gtggcgaccg ccctgtttcc agcatttttc tcgccaccag cgttgtagta
    16201 ttcgcacctt ccagctggga gctggtgatc ggctcttcca ggcgtaactg gcttaactcg
    16261 gctccggccc cgcgtaaccg ttcagtctga tctttcagcg ccagcgcggt agcacgtttg
    16321 tcgacaagct cgcacgcttc tgcaatcacc ggggtaatgt tgaatccggc ctgttcaccc
    16381 gcttcattgc aataattaat tcgttggatc gccgcgttgc gcgccagacg ggtaagcgtc
    16441 cacgccacat cgacgttttc tccttttttg atgcggtact gcaactcatc aaaaggcaga
    16501 taacgtcctt tttcatcggt aacccggtta aagcgcaggt gggccaggag ttcctcaccc
    16561 gataagtgtg ataaaggaat aagtccggga ggtgatttga tagccatgtg cttatctcaa
    16621 aaaaccgcaa tattgagata gtgtatagca ggaaagagaa actatctcaa aaaaagcaag
    16681 ttttgagata gttgaaaaag cggctgacaa ctcgccagcc gttgtgggaa tgatgattac
    16741 atcgctgcgc gataaatcgc cacaatttct tcgtgagttg cctggatcgg gttagtaaag
    16801 ccgcaggcat ctttcagggc attagtcgcc agaaccgcga aatcttcttc tttcacgttc
    16861 aggtcgcgta ggcctgccgg gatatccact ttcttcgcca gttcacggat ggcgttaatg
    16921 caggcttcag caccttccgc gtcgttttta cctgtcacgt tcacgcccat tgcagcggca
    16981 cagtcacgca gacgtgcagc ggcgactttg ctgttgaata cctgaacatg cggcagcaaa
    17041 acggcgttac atacaccgtg tggcaggttg tagaaaccgc ccagctggtg cgccatcgca
    17101 tgaacataac ccagagaagc attattgaac gccataccgg cgaggaactg ggcataagcc
    17161 attgcttcac gcgctttagc attgctgcca tcttcaacgg ctaacggcag gttttcggca
    17221 atcatggtca cggctttcag tgcacaagcg tcagtgatcg gcgtggcggc aatagaaaca
    17281 tacgcttcga tagcgtgcgt taaggcatcc atacccgttg cggcggtcag tgacttcggc
    17341 ataccgatca tcagagagga gtcattgaca gaaagcagcg gagtgacatg tttatccaca
    17401 atcgccattt tgatgtgacg cgcttcgtca gtgatgatgc aaaaacgggt catttcagag
    17461 gccgtacccg ccgtggtgtt gatggcgatc atcggcagct gcggttttgc agagcggtca
    17521 acgccttcat aatcgcggat gtcaccgccg ttggctgcaa ccagcgcaat accttttgcg
    17581 cagtcgtgtg gagaaccacc gcctaaggaa atcacgctat cgcaattatt ttctttaagt
    17641 aatttcaaac ctgcagcgac gttttccgtg gtgggattag gttgggtgcc atcataaata
    17701 acgctaaaaa tattgcgttc ttccagtgct ttttgcacat cacccgccat acccaatttc
    17761 gttaacatat tgtcggtgac aattaaggta cgggtaaatc cataatctgc catcatattc
    17821 attgcatcat tcaatgaatc agcaccaata acattcacgg aaggaataaa aaaggttgaa
    17881 gctgccataa cacactcctg
//
LOCUS       DAHHTG010000090        17747 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.090, whole genome shotgun sequence.
ACCESSION   DAHHTG010000090 DAHHTG010000000
VERSION     DAHHTG010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17747)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17747)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17747
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.090"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            <1..5057
                     /locus_tag="NBI74_003134"
     CDS             <1..5057
                     /locus_tag="NBI74_003134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001335741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="EntS/YbdA MFS transporter"
                     /protein_id="HCD1933422.1"
                     /translation="VNNDGDNAISNGGTGTQINGDDATANNSGKTIVDGKDSTGTEIA
                     GNNAVVNQDGTLDVSGGGHGIDITGDSAKVDNKGGMTVTDPDSIGIQIDGDKAIVNND
                     GDNAISNGGTGTQINGDEATVNNNGNTTVDGKDATGTEIAGNNAVVNQDGTLDVSGGA
                     HGIDITGDGAIANTTGAITVADTDSVGIQVDGNAVTVSNDGDSTITNGGTGTQVNGND
                     AQVNNTGNTTVDGKNSTGTEITGNNGTVNLDGGLIVTGGAYGVEIAGDSAALNNEGDI
                     SVSDEGSIGVLINGEKATVSNTGDVNVSNNATGFDITTNEGNISLAGSMQVGDFSTGM
                     DLNGNNNSVTLAAKDLNVTGQKATGVNVSGDANTINITGNVLVDKDQTADNAADYFYD
                     PSVGINVSGSNNNVTLDGKLTVVADSELTTRNSAGVVVFDGSQENITGLSIAGDGNTF
                     RLNGGIQLVGEANKLTDGSTIASERKGSGKVPVISVDGKSSVHLNGDSTISGDVLLGN
                     TQIIDLRNGAMLDVGADATINMQVDTYERYAMSADHLIDASSGSQLVNNGDINLRSIG
                     FAAITGEDSFGSNTGNLTLSQYLDGLFANGGYGYLATKGASVVNTGTITTKVAEQESV
                     INLGASLGFNDLVFYNAANSMTGLSAKTHGYVLNENGGKIEMFGRGNVGMLAIDESTA
                     ENAGQITLDALWVDVNDTTTLRDNIGNDARGYSVGMGVGTDAGSGPRKNATAVNQQSG
                     VITVYNAGIGMAAYGAGNTVINQGTINLEKNGNYDSSLGVNSLIGMAAYNSGTAINDQ
                     TGVININADNGQAFYSTGNGVILNYGTICVNTNCLTGNDYNDTDSYTSILYTGGDVIT
                     AQSETQSLTKKATISDKKDGNVVNSGALSGADIAISSGKLVNTSTGTINNAIIINGGA
                     LSNEGSVAKVTQNAGTFNNTGSVNSRMTQTGGTFNNQQGGTVKNGSRLANSAIANNMG
                     IWSLGDTSSGGNASMLEINNNAVFKNSGDFILDNSKNAVHLNQSGTFYNTGKMLLSNS
                     SHNGVLNFWGGNGRFINGGIADVTAKSLAVSASDAGSSNAFFWNQSNGVVNFDKDSGV
                     AVKFTHSNYVAQNDGTMNISGNNAIAMEGDKNARLVNNGTINLGTSGTTDTGMIGMQL
                     DSGTTADAVIENNGAINIYANNSFAFSMLGSVGHLVNNGTVTIADGVTGSGLIKQGDS
                     VNIEGVNGNNGNNSEVHYADYTLPDVPGSSVSVAASTPSSDGSQNKLNGYVVGTSSDG
                     SAGKLKVNNASLKGVSVNTGFTAGTSSTSVTFDNVVQGSNLTDAETITSTSVVWNAQG
                     TTDASGNVDVTMTKNAYTDVATDSSVNTVAQVLDAGYTNNELYSSLNVGTTAELNSAL
                     KQVSGSQATTVFNEARVLSNRFSMLSDAAPEVANGLAFNVVAKGDPRAELGNDTQYDM
                     MALRKSLTLTEHQNLSLEYGIARLEGNGSDTAGDNGVTGGYSQFFGLKHQMAFDNGMN
                     WNNALRYDVHQLDSSRSVAYGDINKTADANVKQQYLEFRSEGAKTFELREGLNVTPYA
                     GVKLRHTLEGGYQERNAGDFNLSMNSGSETAVDSIVGLKLDYAGKDGWSANATLEGGP
                     NLSYVKSQRTASVSGAGSQRFNIDDGQNGGGFNSLATMGVKYSSQESALQVDAFHWKE
                     DGISDKGVMLNFKKTF"
     gene            5277..6143
                     /gene="pdxI"
                     /locus_tag="NBI74_003135"
     CDS             5277..6143
                     /gene="pdxI"
                     /locus_tag="NBI74_003135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000097808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 4-dehydrogenase"
                     /protein_id="HCD1933423.1"
                     /translation="MSSNTFTLGTKSVNRLGYGAMQLAGPGVFGPPRDRHVAITVLRE
                     ALALGVNHIDTSDFYGPHVTNQIIREALYPYSDDLTIVTKIGARRGEDASWLPAFSPA
                     ELQKAVHDNLRNLGLDVLDVVNLRVMMGDGHGPAEGSIEASLTVLAEMQQQGLVKHLG
                     LSNVTPTQVAEARKIAEIVCVQNEYNIAHRADDAMIDALARDGIAYVPFFPLGGFTPL
                     QSSTLSDVATSLNATPMQVALAWLLQRSPNILLIPGTSSVGHLRENMASDELVLTDDV
                     LATLDSIAQKAG"
     gene            complement(6183..6788)
                     /gene="azoR"
                     /locus_tag="NBI74_003136"
     CDS             complement(6183..6788)
                     /gene="azoR"
                     /locus_tag="NBI74_003136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HCD1933424.1"
                     /translation="MSKVLVLKSSILAGYSQSNQLSDYFVEQWREKHSADEITVRDLA
                     ANPIPVLDGELVGALRPSDAPLTPRQQEALALSDELITELKAHDVIVIAAPMYNFNIS
                     TQLKNYFDLVARAGVTFRYTEKGPEGLVTGKKAIVITSRGGIHKDGPTDLVTPYLSTF
                     LGFIGITDVKFVFAEGIAYGPEMAAKAQSDAKAAIDSIVAA"
     gene            6989..10891
                     /gene="hrpA"
                     /locus_tag="NBI74_003137"
     CDS             6989..10891
                     /gene="hrpA"
                     /locus_tag="NBI74_003137"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HCD1933425.1"
                     /translation="MTEQQKLTFTALQQRLDSLMLRDRLRFSRRLHGVKKVKNPDAQQ
                     AIFQEMAKEIDHAAGKVLLREAARPEITYPDNLPVSQKKQDILEAIRDHQVVIVAGET
                     GSGKTTQLPKICMELGRGIKGLIGHTQPRRLAARTVANRIAEELKTEPGGCIGYKVRF
                     SDHVSDNTMVKLMTDGILLAEIQQDRLLMQYDTIIIDEAHERSLNIDFLLGYLKELLP
                     RRPDLKIIITSATIDPERFSRHFNNAPIIEVSGRTYPVEVRYRPIVEEADDTERDQLQ
                     AIFDAVDELSQESPGDILIFMSGEREIRDTADALNKLNLRHTEILPLYARLSNSEQNR
                     VFQSHSGRRIVLATNVAETSLTVPGIKYVIDPGTARISRYSYRTKVQRLPIEPISQAS
                     ANQRKGRCGRVSEGICIRLYSEDDFLSRPEFTDPEILRTNLASVILQMTALGLGDIAA
                     FPFVEAPDKRNIQDGVRLLEELGAITTDEQQSAYKLTPLGRQLSQLPVDPRLARMVLE
                     AQKHGCVREAMIITSALSIQDPRERPMDKQQAADEKHRRFHDKESDFLAFVNLWNYLG
                     EQQKALSSNAFRRLCRTDYLNYLRVREWQDIYTQLRQVVKELGIPVNSEPADYREIHI
                     ALLTGLLSHIGMKDADKQEYTGARNARFSIFPGSGLFKKPPKWVMVAELVETSRLWGR
                     IAARIDPEWVEPVAQHLIKRTYSEPHWERAQGAVMATEKVTVYGLPIVAARKVNYSQI
                     DPALCRELFIRHALVEGDWQTRHAFFRENLKLRAEVEELEHKTRRRDILVDDETLFEF
                     YDQRISHDVISARHFDSWWKKVSRETPDLLNFEKSMLIKEGAEKISKLDYPNFWHQGN
                     LKLRLSYQFEPGADADGVTVHIPLPLLNQVEESGFEWQIPGLRRELIIALIKSLPKPV
                     RRNFVPAPNYAEAFLGRVTPLELPLLDSLERELRRMTGVTVDREDWHWDQVPDHLKIT
                     FRVVDDKNKKLKEGRSLQDLKDALKGKVQETLSAVADDGIEQSGLHIWSFGQLPESYE
                     QKRGNYKVKAWPALVDERDSVAIKLFDNPLEQKQAMWNGLRRLLLLNIPSPIKYLHEK
                     LPNKAKLGLYFNPYGKVLELIDDCISCGVDQLIDANGGPVWTEEGFAALHEKVRAELN
                     DTVVDIAKQVEQILTAVFNINKRLKGRVDMTMALGLSDIKAQMGGLVYRGFVTGNGFK
                     RLGDTLRYLQAIEKRLEKLAVDPHRDRAQMLKVENVQQAWQQWINKLPPARREDEDVK
                     EVRWMIEELRVSYFAQQLGTPYPISDKRILQAMEQISG"
     gene            11164..11964
                     /locus_tag="NBI74_003138"
     CDS             11164..11964
                     /locus_tag="NBI74_003138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HCD1933426.1"
                     /translation="MNITPFPTLSPATIDAINVIGQWLAQDDFSGEVPYQADCVILAG
                     NAVMPTIDAACKIARDQQIPLLISGGIGHSTTFLYSAIAQHPHYNTIRTTGRAEATIL
                     ADIAHQFWHIPHEKIWIEDQSTNCGENARFSIALLNQAVERVHTAIVVQDPTMQRRTM
                     ATFRRITGDNPDAPRWLSYPGFVPQLGNNAESVIFVNPLQGLWPVERYLSLLTGELPR
                     LRDDSDGYGPRGRDFIVHVDFPAEVIQAWQTLKHDAVLIEAMESRSLR"
     gene            12174..13613
                     /gene="aldA"
                     /locus_tag="NBI74_003139"
     CDS             12174..13613
                     /gene="aldA"
                     /locus_tag="NBI74_003139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HCD1933427.1"
                     /translation="MSVPVQHPMYIDGQFVTWRGDAWIDVVNPATEAVISRIPDGQAE
                     DARKAIDAAERAQPEWEALPAIERASWLRKISAGIRERASEISALIVEEGGKIQQLAE
                     VEVAFTADYIDYMAEWARRYEGEIIQSDRPGENILLFKRALGVTTGILPWNFPFFLIA
                     RKMAPALLTGNTIVIKPSEFTPNNAIAFAKIVDEIGLPRGVFNLVLGRGENVGQELAG
                     NPKVAMVSMTGSVSAGEKIMATAAKNITKVCLELGGKAPAIVMDDADIELAVKAIVDS
                     RVINSGQVCNCAERVYVQKSIYDQFVNRLGEAMQAVQFGNPAERNDIAMGPLINAAAL
                     ERVEQKVARAVEEGARVALGGKAVEGKGYYYPPTLLLDVRQEMSIMHEETFGPVLPVV
                     AFDTLEEAISMANDSDYGLTSSIYTQNLNVAMKAIKGLKFGETYINRENFEAMQGFHA
                     GWRKSGIGGADGKHGLHEYLQTQVVYLQS"
     gene            complement(13655..14656)
                     /gene="gap"
                     /locus_tag="NBI74_003140"
     CDS             complement(13655..14656)
                     /gene="gap"
                     /locus_tag="NBI74_003140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HCD1933428.1"
                     /translation="MSKVGINGFGRIGRLVLRRLLEVKSNIDVVAINDLTSPKILAYL
                     LKHDSNYGPFPWSVDYTEDSLIVDGKSIAVYAEKEAKNIPWKAKGAEIIVECTGFYTS
                     AEKSQAHLDAGAKKVLISAPAGEMKTIVYNVNDDTLDGNDTIVSVASCTTNCLAPMAK
                     ALHDSFGIEVGTMTTIHAYTGTQSLVDGPRGKDLRASRAAAENIIPHTTGAAKAIGLV
                     IPELSGKLKGHAQRVPVKTGSVTELVSILGKKVTAEEVNNALKKATNNNESFGYTDEE
                     IVSSDIIGSHFGSVFDATQTEITAVGDLQLVKTVAWYDNEYGFVTQLIRTLEKFAKL"
     gene            14845..15375
                     /gene="cybB"
                     /locus_tag="NBI74_003141"
     CDS             14845..15375
                     /gene="cybB"
                     /locus_tag="NBI74_003141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b561"
                     /protein_id="HCD1933429.1"
                     /translation="MGNKYSRLQISIHWLVFLLVIVAYCAMEFRGFFPRSDRPLINMI
                     HVSCGISILVLMVVRLLLRLKYPTPPIIPKPKPMMTGLAHLGHLVIYLLFIALPVIGL
                     VMMYNRGNPWFAFGLTMPYASEANFERVDSLKSWHETLANLGYFVIGLHAAAALAHHY
                     FWKDNTLLRMMPRKRS"
     gene            15620..15793
                     /locus_tag="NBI74_003142"
     CDS             15620..15793
                     /locus_tag="NBI74_003142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1933430.1"
                     /translation="MKKLALILVMGTLVSFYADAGRKPCSGSKGGISHCTAGGKFVCN
                     DGSISASKKTCTN"
     gene            complement(15865..16014)
                     /gene="hokB"
                     /locus_tag="NBI74_003143"
     CDS             complement(15865..16014)
                     /gene="hokB"
                     /locus_tag="NBI74_003143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokB"
                     /protein_id="HCD1933431.1"
                     /translation="MKHNPLVVCLLIICITILTFTLLTRQTLYELRFRDGDKEVAALM
                     ACTSR"
     gene            16277..16720
                     /gene="trg"
                     /locus_tag="NBI74_003144"
                     /pseudo
     CDS             16277..16720
                     /gene="trg"
                     /locus_tag="NBI74_003144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415938.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein Trg"
     gene            complement(16758..17681)
                     /locus_tag="NBI74_003145"
     CDS             complement(16758..17681)
                     /locus_tag="NBI74_003145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCD1933432.1"
                     /translation="MEKNSLFSQRIRLRHLHTFVAVAQQGTLGRAAETLNLSQPALSK
                     TLNELEQLTGARLFERGRQGAQLTLPGEQFLTHAVRVLDAINTAGQSLHRKEGLNNDV
                     VRVGALPTAALGILPSVIGQFHQQQKETTLQVATMSNPMILAGLKTGEIDIGIGRMSD
                     PELMNGLNYELLFLESLKLVVRPNHPLLQENVTLSRVLEWPVVVSPEGTAPRQHSDAL
                     VQSQGCKIPSGCIETLSASLSRQLTVEYDYVWFVPSGAVKDDLRHATLVALPVPGHGA
                     GEPIGILTRVDATFSSGCQLMINAIRKSMPF"
BASE COUNT         4569 a         4195 c         4764 g         4219 t
ORIGIN      
        1 tcgtcaacaa cgacggcgac aacgccatca gcaacggcgg taccggcaca caaatcaacg
       61 gtgacgacgc gaccgcgaac aacagcggca aaaccattgt cgacggcaaa gattccaccg
      121 gtaccgaaat cgcgggcaac aatgcggtgg tgaatcagga cggcacgctg gatgtcagcg
      181 gcggcggtca cggtattgat atcaccggcg acagcgccaa agtggataac aaaggcggta
      241 tgacggttac cgatccggat tccatcggta tccagatcga cggtgataaa gccatcgtca
      301 acaacgatgg cgacaatgct atcagcaacg gcggcaccgg cacgcaaatt aacggcgatg
      361 aagccaccgt gaataataac ggtaacacca ctgttgatgg caaagacgcc accggcacgg
      421 aaatcgcggg taacaacgcg gtggtgaatc aggacggcac gctggatgtc agcggcggtg
      481 ctcacgggat tgatatcact ggagacggcg ccattgcaaa cacgacagga gcgatcactg
      541 tagcggatac ggactctgtc ggtattcagg ttgatggtaa tgcagtgacc gtcagcaatg
      601 acggagacag caccattact aacggcggta cgggcacgca ggttaacggc aacgatgctc
      661 aggtcaacaa tactggcaac accacggtag acggcaaaaa ctccaccgga acagaaatta
      721 cgggcaataa cggcactgta aatctggatg gtggtctgat tgtgacgggt ggtgcctatg
      781 gcgtggagat cgctggcgac agcgccgcgc taaacaatga aggagatatt tcggtttccg
      841 atgagggctc aattggcgta ctcatcaacg gtgaaaaagc aaccgtatct aacacgggtg
      901 atgttaacgt cagcaataac gcgacaggtt ttgacattac cactaatgaa gggaatattt
      961 cgctggcagg cagtatgcag gtgggcgact tctctaccgg gatggatcta aacggtaata
     1021 acaacagcgt cacgctggcg gcaaaagatc tgaacgtcac cggtcagaag gcgacaggcg
     1081 tgaatgtatc cggcgatgcc aacactatta atatcaccgg taatgttctg gtagataaag
     1141 atcaaacggc tgacaacgcg gcagattatt tctacgaccc gtctgtgggg attaatgtca
     1201 gcggcagtaa taacaatgtc actctggacg gtaagttaac ggttgttgct gacagtgaac
     1261 ttactacacg caattctgct ggcgtcgttg tatttgatgg tagccaggag aatattaccg
     1321 gactcagcat tgcaggcgat ggtaatactt tccgtctgaa tggcggtatt caactggttg
     1381 gtgaggcgaa caagctaact gacggttcca ctattgcgtc cgagcgaaaa ggcagtggca
     1441 aggttcctgt tatcagtgtt gatggaaaat cgtccgttca tctgaacggg gattccacta
     1501 ttagcggtga tgtgctgctc ggcaatacac aaatcattga tttgcggaat ggtgccatgc
     1561 tggatgtcgg tgcggatgcc acgatcaaca tgcaggttga tacttacgag cgttatgcta
     1621 tgtcagccga tcacctcatt gatgcatcgt ctggctctca gttggtcaat aacggtgata
     1681 ttaatctccg aagcatcggt tttgccgcta ttactggtga ggattcattc ggaagcaaca
     1741 ctggcaatct cacgctttct cagtacctcg atgggctgtt tgcaaacgga gggtatggct
     1801 atttagccac caaaggggcc agtgtcgtga acacaggcac tatcactacg aaagtggcgg
     1861 aacaagagag cgtaatcaac cttggcgcgt cactgggttt taatgattta gtcttttata
     1921 acgcagcgaa cagtatgacg ggccttagcg ccaaaactca tggttatgta ctcaatgaga
     1981 acggcggcaa aatcgagatg ttcggacgtg gcaatgtcgg tatgttggcc atcgatgaaa
     2041 gtacggcaga aaacgcgggg cagattacac tggatgcact gtgggttgat gtaaacgata
     2101 caacaacttt gcgtgacaat attggtaacg acgccagagg atattctgtt ggtatgggcg
     2161 ttgggacaga tgctggcagt ggaccgagga agaacgccac agcagtcaac caacagagtg
     2221 gtgttatcac cgtttataac gccggaattg gtatggccgc ctacggcgct ggtaatacgg
     2281 ttattaacca gggcaccatt aatctggaga aaaacggtaa ttacgatagt agcctgggcg
     2341 tgaattcgct tatcggtatg gctgcatata acagtggtac cgccattaac gatcaaaccg
     2401 gtgttatcaa tattaatgct gacaatgggc aggcatttta tagcactggt aacggtgtca
     2461 ttcttaacta tggcaccatt tgtgttaaca ctaattgcct gacgggcaat gactataatg
     2521 atactgactc atatacctcg atattataca cgggtggcga tgtcatcact gcacagagtg
     2581 aaacacaaag tctgaccaaa aaagccacga ttagcgacaa aaaagacggt aatgttgtta
     2641 acagtggtgc gctttccggt gctgatattg cgattagtag cggtaagtta gttaatacat
     2701 caacgggtac gattaataac gcaataatta tcaacggcgg tgcgttaagt aacgaaggct
     2761 ctgtggcgaa ggtgacgcaa aacgccggta ctttcaataa cacagggtcg gttaacagtc
     2821 gtatgactca gacaggagga acgttcaata accaacaggg cgggacggtg aagaacggct
     2881 ccagactggc caacagcgct attgctaata acatggggat ctggtccctt ggcgatacga
     2941 gtagtggtgg taatgccagc atgctggaaa ttaataataa tgccgtgttc aaaaacagtg
     3001 gtgacttcat tctggataat agcaaaaacg ctgttcatct gaatcagtca ggtacgttct
     3061 acaataccgg gaaaatgttg ctatccaact cttcccataa cggcgtacta aatttctggg
     3121 gtgggaatgg gcgctttatt aacggcggta ttgcggatgt cacggcgaaa tctttggccg
     3181 taagcgccag tgatgccggg agcagcaatg ccttcttctg gaaccagagc aatggggtcg
     3241 tcaattttga taaagacagc ggcgttgcag tgaaattcac tcacagcaat tacgttgcgc
     3301 agaatgacgg caccatgaat attagcggta ataacgctat tgcgatggaa ggggataaaa
     3361 acgcacgact ggttaacaac ggcacgatca acctcggaac gtcgggcacg accgacacgg
     3421 gcatgatcgg gatgcaactg gacagcggca cgacagctga tgcggtcatt gagaacaatg
     3481 gcgccatcaa catttacgcc aacaactcct ttgcatttag catgttgggt tccgtaggcc
     3541 acctcgtgaa caacggcaca gtgactattg ccgatggagt gacaggttcc gggctaatca
     3601 agcaaggtga cagcgttaac attgaaggtg tgaacggcaa taacggtaac aatagcgaag
     3661 tgcattatgc tgattacact ttgcctgatg tacctggctc gtcggtttct gttgcggcca
     3721 gcaccccgtc cagtgacgga agccagaaca aactgaatgg ttatgttgta ggaaccagtt
     3781 ctgacggcag tgccggcaag ctgaaagtga acaatgcaag cctgaaaggg gtttctgtta
     3841 acaccggatt tacggctgga accagctcca cctccgtgac cttcgacaac gtcgttcagg
     3901 gcagtaatct gaccgacgca gagacgatta catcgacctc tgtggtatgg aacgcgcaag
     3961 gcactactga cgccagcggc aacgttgatg tcacgatgac taaaaatgcc tacaccgatg
     4021 tagcaacaga ttcatctgtt aacaccgtcg cgcaggtact ggatgcgggc tataccaaca
     4081 acgagttgta cagcagcctg aacgtaggca ccacggcgga actgaacagt gcgctgaaac
     4141 aggtcagcgg cagccaggca acaacggtat tcaatgaagc acgagtgtta agtaaccgct
     4201 tcagcatgtt gtccgatgca gcaccggaag tcgcaaacgg tctggccttc aacgtggtag
     4261 cgaaaggcga cccgcgtgct gaactgggta acgacacaca gtacgacatg atggcgctgc
     4321 gtaaatctct gacgctgact gaacatcaga acctgagtct ggaatacggg attgcgcgtc
     4381 tggagggtaa tggttcagat accgcaggcg ataacggagt gacaggcggt tacagccagt
     4441 tcttcggtct gaaacaccag atggcctttg acaacggtat gaactggaat aacgccctgc
     4501 gttatgatgt gcaccagctc gacagcagcc gctcggtagc gtacggcgat atcaataaaa
     4561 cagccgatgc taatgtgaaa caacagtacc tggagttccg tagtgaaggg gcgaaaacct
     4621 ttgagctgcg tgaagggctg aacgtcacgc catatgccgg ggttaaactg cgtcacacac
     4681 tggagggtgg ctatcaggaa cgcaatgcgg gtgactttaa cctgagcatg aacagcggta
     4741 gcgaaacggc agtggacagc attgttgggc tgaaactgga ctatgccggt aaggacggct
     4801 ggagtgcgaa cgccacgctg gaaggcggcc cgaacctgag ctatgtgaaa agccagcgaa
     4861 ctgcgagcgt ttcgggcgcg ggaagccagc gtttcaacat tgatgatggt cagaatggtg
     4921 gtggtttcaa cagcctggcg acaatgggtg tgaaatacag cagccaggaa agtgcgttgc
     4981 aggtagatgc attccactgg aaagaagacg gcatcagtga taaaggtgtg atgctaaact
     5041 tcaagaaaac gttctaattt ttagcatgta atgcataaac cgcaacgctg atacaggttg
     5101 cggttttttt attgccggat gcggtgcttg acgtgtctta acctgcctgg ggtttcgtgt
     5161 ctttcagggc aacagtacaa ctcatcccgc aatcttgttt acaccctcgc attatttatc
     5221 tcttttcgtt tctatactga tttttcttaa tccgttttat tacagggcag ggtgcgatga
     5281 gcagcaacac atttactctc ggtacaaaat ccgttaaccg tcttggttat ggcgcgatgc
     5341 aactggctgg tcctggggtt tttggccccc caagagatcg ccacgtcgct ataaccgtgc
     5401 tgcgcgaggc gctggcattg ggcgtcaatc atattgatac cagcgacttt tatggtccgc
     5461 acgtcaccaa tcagattatc cgcgaagcgc tttatcctta ctctgacgac ctgacaattg
     5521 tcactaaaat tggtgcgcgg cgtggagagg acgcatcctg gttacccgca ttttctccgg
     5581 cagagctgca aaaagcggta cacgataatc tacgtaatct cggcctggac gtactggatg
     5641 tggttaacct gcgcgttatg atgggggatg gtcatggccc agcggaagga tcgattgagg
     5701 ccagcctgac agtgctggcg gagatgcaac aacaaggtct ggtaaaacat cttggcctga
     5761 gtaacgtcac accgacgcag gttgcagagg cgcgaaagat tgccgaaatc gtctgcgtgc
     5821 agaacgaata caacattgcc caccgtgctg atgacgcaat gattgatgct ttggctcgtg
     5881 atggcattgc ctacgtgccg ttcttcccgc tcgggggctt tacgccgctg caatcctcca
     5941 cgctttccga tgttgctaca agccttaatg cgacaccaat gcaggtggcg ctggcgtggc
     6001 tgttacagcg ttcaccgaat attttgctga ttcctggcac ctcttcggtg gggcatctgc
     6061 gggagaatat ggcgtccgat gaattagtgc taacggatga cgtgctggca acgctggaca
     6121 gtattgcgca aaaggcgggt taaataaaaa acctcccact ttcgtgggag gcatcataaa
     6181 tcttatgcag caacaatact gtcgatggct gcttttgcgt cagactgtgc tttcgctgcc
     6241 atttccgggc cgtacgcgat cccttcggcg aagacaaatt tcacatcggt aatgccgata
     6301 aagcccagga atgtggacag atatggcgtt accagatccg ttgggccatc tttatggatc
     6361 ccaccgcggc tggtgataac gatggctttt ttaccggtga ccaggccttc cggacccttc
     6421 tcggtatagc ggaaagtaac gcctgcacgt gcaaccaggt caaaatagtt tttcaactgg
     6481 gtcgagatat tgaagttata catcggtgcc gcaataacga taacgtcgtg ggctttcagc
     6541 tcggtaatca gctcatcgga aagcgccaga gcttcctgct gacgcggagt cagcggcgca
     6601 tcgctcggac gcagtgcacc aaccagttca ccatccagga ccggaatcgg atttgcagcc
     6661 aggtcacgca cggtgatttc atcagcggag tgcttttcgc gccattgttc aacaaaataa
     6721 tcggacaact gattagactg agagtaccct gccaggatgc tggatttaag aactaatacc
     6781 ttgctcatgg tgtttcctta tagatatttg aatgggcaat gccccgttgc ttgttgacac
     6841 tttattcaca atcctgccac agagatagcg caataaatcg aagcctatgt tcgaatttat
     6901 tgaacaacgc atagaaagct gcgatgtggt actctatatc tatcatttaa aagaaaatta
     6961 atcaggcagc ctactgccca ctaacgttat gacagaacaa caaaaattga cctttactgc
     7021 cttgcagcag cggctggatt cgttgatgct gcgtgacaga ttgcgttttt ctcgccgtct
     7081 gcacggtgtg aagaaggtta aaaatcctga tgcacaacag gccattttcc aggagatggc
     7141 gaaagagatt gaccatgcgg cagggaaagt cctgctgcgt gaagcggcac gaccggagat
     7201 tacttacccg gacaatttgc cggttagtca gaaaaagcag gacattctcg aagcgattcg
     7261 tgaccaccag gtggtgatcg tcgccgggga aacgggttcc ggtaaaacga ctcagctacc
     7321 gaaaatctgt atggagctgg ggcgcgggat taaagggctg atcggccata cccagccgcg
     7381 tcggctggcg gcgcgaacgg tggcgaaccg tattgcggaa gagctaaaaa cggagccggg
     7441 cggttgcatc ggttacaaag tgcgtttcag cgatcacgtc agtgataaca cgatggtcaa
     7501 gctgatgacc gacggtatcc tgctggcgga gatccagcaa gaccgcctgc tgatgcagta
     7561 cgacaccatc attattgacg aagcgcacga acgcagcctg aatatcgatt ttctgctcgg
     7621 ctatttgaaa gagttgctgc cgcggcgtcc tgacctaaaa atcattatca cttccgcgac
     7681 tatcgacccg gaacgctttt cgcgccactt taataatgcg ccgattatcg aagtctccgg
     7741 tcggacctat ccggtggaag tgcgctatcg cccgattgtt gaagaagccg atgataccga
     7801 gcgcgatcag ttgcaggcga tttttgacgc cgtagacgaa ctgagccagg aaagccctgg
     7861 cgacattctg atctttatga gcggcgagcg agaaattcgc gataccgccg atgcgctgaa
     7921 caagctgaac ttacgccata ccgaaatctt gccgctttat gcacggcttt ccaacagcga
     7981 acagaaccgc gtgttccagt cgcacagcgg gcgacgcatt gtactggcga ccaacgtcgc
     8041 ggaaacctcg ctgaccgtac cgggtattaa atacgttatc gacccaggta cagcgcgtat
     8101 cagccgctac agctatcgca ccaaagtgca gcgtttgccg attgagccaa tttcgcaggc
     8161 ttcagctaat cagcgtaaag gccgctgtgg tcgtgtgtcc gaagggatct gtattcgtct
     8221 ctattccgaa gacgatttcc tttcgcgccc ggagtttacc gatccggaga ttctgcgtac
     8281 caacctggcc tcggttattt tgcagatgac cgcactgggc ttgggcgata tcgccgcatt
     8341 cccgtttgtc gaagcaccgg ataaacgcaa tatccaggat ggcgtgcgtc tgctcgaaga
     8401 actgggcgcg atcaccactg atgaacagca aagtgcttat aaactgacgc cgctcggtcg
     8461 tcagctctcg cagttgcctg tcgacccacg tctggcgcgt atggtgctgg aagcgcaaaa
     8521 acatggctgc gtgcgtgagg cgatgattat cacgtccgcg ctctccattc aggatccgcg
     8581 cgagcgtccg atggataaac agcaggcagc tgacgaaaaa catcgtcgct tccacgacaa
     8641 agagtctgac ttcctggcat ttgtgaatct gtggaattat cttggcgagc agcaaaaggc
     8701 gctttcttca aacgccttcc gtcgcctgtg tcgtaccgat tatctcaact atctgcgcgt
     8761 gcgcgaatgg caggatatct acacccagtt gcgccaggtg gtgaaagaac ttggcattcc
     8821 ggttaacagc gaaccggcgg attatcgcga aattcacatt gcgttgctga ccggtttgct
     8881 ttcgcatatc ggcatgaaag atgccgataa acaagaatat accggcgcac gtaacgcgcg
     8941 tttctccatc ttccccggtt ctggtttatt caaaaagccg ccgaaatggg taatggtggc
     9001 ggaactggta gaaaccagcc gcctgtgggg gcgcattgct gcgcggatcg acccggaatg
     9061 ggtggagcca gttgctcagc atttgattaa acgcacctac agcgaaccgc actgggaacg
     9121 ggcgcagggc gcggtgatgg caacggaaaa agtcactgtt tatggtttgc cgattgttgc
     9181 cgcgcgcaag gtcaactaca gccagatcga tccggcgtta tgtcgtgaac tctttattcg
     9241 ccacgcgctg gtggaaggtg actggcagac gcgtcacgca ttcttccgcg aaaacctgaa
     9301 actacgggcg gaagtagaag agctggaaca caaaactcgt cgccgcgata ttctggtgga
     9361 tgacgaaacg ttgtttgagt tctacgacca gcgcatcagt cacgatgtga tctctgcacg
     9421 ccatttcgac agttggtgga aaaaagtcag ccgcgaaacg cctgatttgc tcaactttga
     9481 aaagagcatg ttgatcaaag aaggggcgga aaaaatcagc aagctggatt acccgaactt
     9541 ctggcatcag ggcaatctca agctgcgttt gagttatcag tttgagccgg gggcagatgc
     9601 tgacggcgtg accgtacata ttccgctgcc gctgcttaac caggttgagg agagcgggtt
     9661 tgagtggcag atccccggcc tgcgccgtga gctgatcatc gccctgatta aatcgttgcc
     9721 gaagccggta cgccgtaatt ttgtacctgc gccaaactat gccgaagcgt ttttaggccg
     9781 cgtcacaccg ctggaattgc cgttgctcga cagccttgaa cgagagcttc ggcggatgac
     9841 tggcgtcacc gttgaccgcg aagactggca ctgggatcag gtgcccgatc acctgaaaat
     9901 caccttccgc gtggtggatg acaaaaacaa gaagctaaaa gaagggcgct cattacagga
     9961 tctgaaagac gccctgaaag gcaaagtgca ggaaacgctg tctgccgtgg cggatgacgg
    10021 tatcgagcag agcggtttac atatctggag ttttggtcaa ctgccggaaa gctacgagca
    10081 gaagcgcggc aactacaaag tgaaggcttg gccggcgctg gtggatgagc gtgacagcgt
    10141 agcgatcaaa ctgtttgata acccgctgga acagaagcag gcaatgtgga acggtcttcg
    10201 ccgtttactg ctgctaaata ttccatcgcc gatcaagtac ttgcacgaaa agctgccaaa
    10261 caaagccaag ctggggctgt actttaaccc gtatggcaaa gtgctggagc tgatcgatga
    10321 ctgtatctcc tgcggtgtgg atcaactgat cgacgccaat ggtggcccgg tctggacgga
    10381 agaaggcttt gctgcgctgc atgaaaaagt ccgtgctgaa ctgaacgaca cggtggtgga
    10441 tattgcgaag caggtcgagc aaatcctcac tgcggtgttc aatatcaaca aacgtctgaa
    10501 agggcgggtg gatatgacca tggcgctggg gctttctgac attaaagcgc agatgggcgg
    10561 gctggtgtat cgcggttttg tcactggtaa cggcttcaaa cgactgggcg acacgctgcg
    10621 atatttgcag gcaattgaaa aacgactgga aaaactggcg gtcgatccac atcgcgaccg
    10681 tgctcagatg ctgaaagtcg aaaacgtcca gcaggcgtgg cagcaatgga tcaacaaact
    10741 accgcctgca cgtcgtgagg atgaagacgt gaaagaggtc cgctggatga tagaagagct
    10801 gcgcgttagt tacttcgctc aacaacttgg cacgccttat ccgatttcgg ataagcgtat
    10861 tttgcaggcg atggagcaga ttagcggtta acactgctat ttgcctgata aatctaaaac
    10921 ccggtaagca tttagcgccg ggttttttat taattctaaa aacgataagg gaaaaaattc
    10981 aggagttcag aaaaatacaa ttctctgctg caagatgaat aatgtttatc tacagcattt
    11041 ccttaaaaga tatgtcaggc ttgcggagtg gcagttcagg aaatacgact tcctcctttc
    11101 agagtgctcc gcttctcact attatctcac gcagtattct taagggaacg ataaggagga
    11161 accatgaata ttaccccgtt tccgacgctt tcgccggcaa ccatagatgc cataaatgtt
    11221 atcggacagt ggctggcgca ggatgatttc tccggtgagg tgccgtatca ggccgattgc
    11281 gtgatccttg caggcaatgc ggttatgccg actatcgatg cggcatgtaa gattgcccgc
    11341 gatcagcaaa ttcctttact gattagtggt ggtatcggtc actcgacaac ttttttgtat
    11401 agcgccatcg cacagcatcc gcactacaac actatccgca ccactggcag agcagaagct
    11461 accattctgg cggatatcgc tcatcagttc tggcatattc cgcatgaaaa aatttggatt
    11521 gaagaccagt caacaaactg cggtgaaaac gcacgcttta gcatcgcgtt attgaatcag
    11581 gctgtagaac gagttcatac ggctatcgtt gttcaggacc ccaccatgca gcggcgcacg
    11641 atggcgacgt tccgccgtat aaccggggat aatcccgatg caccacgctg gttaagttat
    11701 cccggattcg ttcctcagtt aggaaataac gcagagagtg taatctttgt taatccgttg
    11761 caaggattat ggccggttga gcgttatcta tcgctactca ctggcgagct gccgcgttta
    11821 cgcgatgata gcgatggcta cggcccccgc gggcgagatt ttatcgttca cgttgatttt
    11881 ccggcagaag tcattcaggc atggcaaacg ctgaaacatg atgcggtgct catcgaggcg
    11941 atggaaagtc gctcgttacg ttaaaaattg cccgtttgtg aaccacttgt ttgcaaacgg
    12001 gcatgatttc tgacttttat ttctgccttt tatttctgcc tttaattcct tttacacttg
    12061 tttttatgaa gcccttcaca gaattgtcct ttcacgatgc cgtctctctg atgattgatg
    12121 ttaattaaca ataaatttac caaaaacaaa catgcaaatc acaggagtcg cccatgtcag
    12181 tacccgttca acatcctatg tatatcgatg gacagtttgt tacctggcgt ggagacgcat
    12241 ggattgatgt ggttaaccct gctacagagg ctgtcatttc ccgcattccc gatggtcagg
    12301 ccgaggatgc ccgtaaggca atcgatgcag cagaacgtgc acaaccagaa tgggaagcgt
    12361 tgcctgctat tgaacgcgcc agttggttgc gcaaaatctc cgccgggatc cgggaacgtg
    12421 ccagtgaaat cagtgcgctg attgtcgaag aagggggcaa gatccagcag ttggctgaag
    12481 tcgaagtggc ttttactgcg gactatatcg attatatggc ggagtgggca cggcgttacg
    12541 agggcgagat tattcaaagc gatcgtccag gagaaaatat ccttctgttt aaacgtgcgc
    12601 ttggcgtgac taccggcatt ctgccgtgga actttccgtt cttcctcatt gcccgcaaaa
    12661 tggctcccgc tcttttgacc ggtaatacca tcgtcattaa acccagtgaa tttacgccaa
    12721 acaatgcgat tgcattcgcc aaaatcgttg atgaaatagg ccttccgcgc ggcgtgttta
    12781 accttgtact cgggcgtggt gaaaacgttg ggcaagaact ggcgggtaac ccaaaggttg
    12841 caatggtcag tatgacaggc agcgtctctg caggtgaaaa gattatggcg actgcggcga
    12901 aaaacatcac caaagtgtgc ctggaattgg ggggtaaagc accggctatc gtaatggacg
    12961 atgccgatat tgaactggca gtcaaagcca tcgttgattc acgcgtcatt aatagtgggc
    13021 aagtgtgtaa ctgtgcagaa cgtgtttatg tacagaaaag catttatgat cagttcgtca
    13081 atcggctggg tgaagcgatg caggcggttc aatttggtaa ccctgctgaa cgcaacgaca
    13141 ttgcgatggg accgttgatt aacgccgcgg cgctggaaag ggttgagcaa aaagtggcgc
    13201 gcgcagtaga agaaggggcg agagtggcgt tgggtggcaa agcggtagag gggaaaggat
    13261 attattatcc gccgacattg ctgctggatg ttcgccagga aatgtcgatt atgcatgagg
    13321 aaacctttgg tccggttctg ccggtggtcg catttgacac gctggaagag gctatctcaa
    13381 tggctaatga cagtgattac ggcctgacct catcaatcta tacccaaaat ctgaacgtcg
    13441 cgatgaaagc cattaaaggg ctgaagtttg gtgaaactta catcaaccgt gaaaacttcg
    13501 aagctatgca aggcttccac gctggatggc gtaaatccgg tattggcggc gcagatggta
    13561 aacatggctt gcatgaatat ctgcagaccc aggtggttta tttacagtcg tagtgagtga
    13621 aagaggcgga ggttttttcc tccgcctgtg cgcgtcagag tttagcgaat ttttcgaggg
    13681 tgcgaataag ctgcgtgacg aagccatatt cgttatcgta ccaggcgacc gttttcacca
    13741 gttgtaaatc gcccacggcg gtaatttccg tttgcgtggc atcaaacacc gaaccgaaat
    13801 ggctgccaat gatatcggaa gagactattt cttcatcggt ataaccaaat gactcgttat
    13861 tgtttgtcgc ctttttaagc gcgttgttca cctcttcggc agtcactttt tttccgagaa
    13921 tcgacaccag ttcagtgacc gaacctgttt tcaccggcac gcgttgcgca tgacctttca
    13981 gtttgccgct cagttccggg atcaccaggc caatggcttt tgccgccccc gtagtgtggg
    14041 gaatgatatt ttctgccgct gcgcgtgaag cacgtaaatc tttaccacgc gggccatcca
    14101 ccagtgactg ggtgccggta taggcatgaa tggtcgtcat cgtgccgact tctatcccga
    14161 aactgtcatg caaggctttg gccatcggcg caaggcagtt agtggtgcat gacgccacgg
    14221 aaacaatggt gtcgttgcca tccagagtgt cgtcattgac gttataaacg atggttttca
    14281 tttcaccggc aggggcggaa atcaacacct tcttcgcgcc agcatcaaga tgcgcctgcg
    14341 atttctcggc ggaggtataa aagcctgtac attcgacaat gatttctgcg cctttcgctt
    14401 tccacggaat atttttagcc tctttttcgg cgtaaaccgc gatacttttc ccatcaacga
    14461 taagtgaatc ttccgtataa tcaacgctcc aggggaacgg tccgtagttt gaatcatgtt
    14521 tcagcaggta ggcgagaatt tttggggaag tgagatcatt aatagcgaca acgtctatgt
    14581 tgcttttgac ttcaagtaat cgacgcaaca ccagtcgacc gatacgacca aaaccattaa
    14641 taccaacttt actcatggtc ttctcctgtc aggaacgttc ggatgaaaat tgatcctttc
    14701 caggcttaga ccaggatggc gggatgggca atctacattc ttgcagtgaa acgtaacgta
    14761 ctgaaaacgg gtgaacaata tttaatgaaa ttttaagaaa agcccgttat gttaacggca
    14821 aattatgtta aagcaggaaa tgttatggga aataaatact caaggttaca aatcagcatt
    14881 cactggctgg tctttttgct ggttatcgta gcgtattgcg caatggagtt tcgtggtttc
    14941 ttcccacgta gcgatcggcc cctcatcaac atgattcatg tttcctgtgg catctcaatt
    15001 ctcgtactga tggtggttcg tcttttgtta aggctgaaat acccaacccc gccgattata
    15061 cctaagccaa aaccgatgat gacgggactg gcgcatttgg gccatttggt gatttatctc
    15121 ctgtttattg cgctgccggt gattggtttg gtgatgatgt ataaccgggg caacccgtgg
    15181 tttgcgtttg gtttgacgat gccttacgct tcagaggcca atttcgagcg ggttgatagc
    15241 ttaaagtcgt ggcatgaaac gctggcgaat ctgggatatt ttgtcatcgg gttgcacgct
    15301 gcggctgcac tggcacacca ctatttctgg aaggacaaca cacttctacg catgatgcca
    15361 cgtaaacgtt cctgaaggat atttaaagaa aacgcctgta ctaaaaccga cccgtggtac
    15421 aggcgaagaa tacgggccta catcggaagc gcctattata tttatttgta tgacaaataa
    15481 aaccataatc cttgtctata cgtccatctg gcttatcttt aatcaattca cccgatcttt
    15541 gatctcatca acggtatcaa aataaaccag cgtataacgt tcaaatcgaa cataagattc
    15601 gataaatcat ggaggttata tgaaaaaact ggcacttatc ctggttatgg gaacgcttgt
    15661 ttccttttat gccgatgccg ggcgcaaacc ctgttctggt tcgaaagggg ggatctcaca
    15721 ctgtacggca ggcggcaaat ttgtctgtaa tgatggttct attagtgcat cgaaaaaaac
    15781 atgcactaac tgaagtgtaa aaggggggcc atgagaaaat gaatccgcta gtcagatgcg
    15841 cggggaaaac ccccgcgctt gcccttacct ggacgtgcag gccatgagcg cagcaacctc
    15901 cttatcaccg tcccggaacc gcagttcgta gagcgtttgt cgggtcagga gtgtgaatgt
    15961 cagaatcgta atgcagataa tgagcagaca caccaccaga gggttgtgct tcatagcctt
    16021 tctccttgcc ggatggcggg taagaggcta agatctgaat tgctaggttc attcgttggc
    16081 ctcggttgat agaaatatcg gtcggggcct tcgtctttct gattccaggt tagcctgaaa
    16141 acagaaagtc tcaggcaccc gtgagcatcc tatgtggttt ccttagggac gaaaataatc
    16201 acctgacgaa atcgcgtgct gctttccaga atttttcgtc attcggttta gccagtttaa
    16261 tcattcgtta ctctcgcaag gccgtggatt tgccgttgtc gccagcgaag tacggacact
    16321 cgcaagccgc agcgcccagg cggcgaaaga gattgaaggc ttgatcagtg aatcagtcag
    16381 gttaattgac ctgggatcgg atgaggtggc aacggcaggg aaaaccatgt gcactattgt
    16441 tgatgccgtc gcgagtgtca cacatatcat gcaggaaatc gccaccgcct cgggtgaaca
    16501 aagtcgaggc ataacgcagg ttagccaggc gatttctgaa atggataagg tgacgcaaca
    16561 gaatgcttct ctggtagaag aggcctcaac ggcggcggtg tcccttgaag aacaggcggc
    16621 acgactaact gaggcggtgg acgtattccg tctgaacaaa cattctgtgt cggcagaacc
    16681 acgaggagcg ggtgaaccag ttagttttgc tacggtgtga aaatgttaaa ggagggatcg
    16741 acagatccct tcacctttca gaacggcatt gattttcgaa tagcgttaat catcaactgg
    16801 caaccagaag agaacgtcgc atctacgcgg gtcagtattc caatcggttc gcctgcacca
    16861 tgtcccggaa caggcagggc taccagcgtg gcatgacgca ggtcgtcttt tacagcgcca
    16921 gaagggacaa accacacgta atcgtattca accgtaagtt gacgagatag cgaagcagac
    16981 agcgtttcga tacaacccga aggaatttta catccctggc tctgcactaa tgcatctgaa
    17041 tgctggcgtg gcgcagtgcc ttctggtgat acaacgaccg gccattccag cacccggctt
    17101 agcgttacgt tctcctgaag tagcgggtga ttagggcgga caaccagctt cagcgattca
    17161 agaaacagta gttcgtaatt aagcccgttc atcagttcag gatctgacat ccgaccaatg
    17221 ccgatatcga tttccccggt ttttaaaccc gccagaatca tagggttact cattgtcgca
    17281 acttgcaagg tcgtctcttt ttgttgctga tgaaactgac ctataaccga aggtaatatc
    17341 cctagtgccg cagtaggtag cgcaccaacc ctgacgacat cattattaag accttcttta
    17401 cgatgaagcg actgtccggc agtgttgatg gcgtcaagaa ctctgactgc atgcgttaaa
    17461 aattgttcgc cgggtaaggt aagttgcgcc ccctgacgac cacgctcaaa caagcgagcg
    17521 cccgtcagct gctccagttc attcaatgtc ttagagagcg caggttgact caaattaagg
    17581 gtttcagccg cgcgccccaa agttccttgt tgtgcgacag ctacgaatgt atgaaggtgg
    17641 cgcaaacgga tgcgctgact aaacagacta tttttttcca taagcgatgt taaaaacgaa
    17701 gcggtgtcgc tgacaagtga agttgtttta ttatgataac ttgattg
//
LOCUS       DAHHTG010000091        17699 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.091, whole genome shotgun sequence.
ACCESSION   DAHHTG010000091 DAHHTG010000000
VERSION     DAHHTG010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17699)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17699)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17699
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.091"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            98..601
                     /locus_tag="NBI74_003146"
     CDS             98..601
                     /locus_tag="NBI74_003146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbM family protein"
                     /protein_id="HCD1933433.1"
                     /translation="MGMIGYFAEIDSEKINQLLESTKKPLMDNIHDTLSGLRRLDIDK
                     RWDFLHFGLTGTSAFDPAKNDPLSRAVLGEHSLEDGIDGFLGLTWNQELAATIDRLES
                     LDRSELRKQFSIKRLNEMEIYPGVTFSEELEGQLFASIMLDMEKLISAYRRMLRQGNH
                     ALTVIVG"
     gene            complement(668..2125)
                     /gene="nuoN"
                     /locus_tag="NBI74_003147"
     CDS             complement(668..2125)
                     /gene="nuoN"
                     /locus_tag="NBI74_003147"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HCD1933434.1"
                     /translation="MTITPQNLIALLPLLIVGLTVVVVMLSIAWRRNHFLNATLSVIG
                     LNAALVSLWFVGQAGAMDVTPLMRVDGFAMLYTGLVLLASLATCTFAYPWLEGYNDNK
                     DEFYLLVLIAALGGILLANANHLASLFLGIELISLPLFGLVGYAFRQKRSLEASIKYT
                     ILSAAASSFLLFGMALVYAQSGDLSFVALGKNLGDGMLNEPLLLAGFGLMIVGLGFKL
                     SLVPFHLWTPDVYQGAPAPVSTFLATASKIAIFGVVMRLFLYAPVGDSEAIRVVLAII
                     AFASIIFGNLMALSQTNIKRLLGYSSISHLGYLLVALIALQTGEMSMEAVGVYLAGYL
                     FSSLGAFGVVSLMSSPYRGPDADSLFSYRGLFWHRPILAAVMTVMMLSLAGIPMTLGF
                     IGKFYVLAVGVQAHLWWLVGAVVVGSAIGLYYYLRVAVSLYLHAPEQPGRDAPSNWQY
                     SAGGIVVLISALLVLVLGVWPQPLISIVRLAMPLM"
     gene            complement(2132..3661)
                     /gene="nuoM"
                     /locus_tag="NBI74_003148"
     CDS             complement(2132..3661)
                     /gene="nuoM"
                     /locus_tag="NBI74_003148"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HCD1933435.1"
                     /translation="MLLPWLILIPFIGGFLCWQTERFGVKVPRWIALVTMGLTLALSL
                     QLWLQGGYSLTQSAGIPQWQSEFDMPWIPRFGISIHLAIDGLSLLMVVLTGLLGVLAV
                     LCSWKEIEKYQGFFHLNLMWILGGVIGVFLAIDMFLFFFFWEMMLVPMYFLIALWGHK
                     ASDGKTRITAATKFFIYTQASGLVMLIAIQALVFVHYNATGVWTFNYEELLNTPMSNG
                     VEYLLMLGFFIAFAVKMPVVPLHGWLPDAHSQAPTAGSVDLAGILLKTAAYGLLRFSL
                     PLFPNASAEFAPIAMWLGVIGIFYGAWMAFAQTDIKRLIAYTSVSHMGFVLIAIYTGS
                     QLAYQGAVIQMIAHGLSAAGLFILCGQLYERIHTRDMRMMGGLWSKMKWLPALSLFFA
                     VATLGMPGTGNFVGEFMILFGSFQVVPVITVISTFGLVFASVYSLAMLHRAYFGKAKS
                     QIASQELPGMSLRELFMILLLVVLLVLLGFYPQPILDTSHSAIGNIQQWFVNSVTTTR
                     P"
     gene            complement(3825..5666)
                     /gene="nuoL"
                     /locus_tag="NBI74_003149"
     CDS             complement(3825..5666)
                     /gene="nuoL"
                     /locus_tag="NBI74_003149"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HCD1933436.1"
                     /translation="MNMLALTIILPLIGFVLLAFSRGRWSENVSAIVGVGSVGLAALV
                     TAFIGVDFFANGEQAYSQPLWTWMSVGDFNIGFNLVLDGLSLTMLSVVTGVGFLIHMY
                     ASWYMRGEEGYSRFFAYTNLFIASMVVLVLADNLLLMYLGWEGVGLCSYLLIGFYYTD
                     PKNGAAAMKAFVVTRVGDVFLAFALFILYNELGTLNFREMVELAPAHFADGNNMLMWA
                     TLMLLGGAVGKSAQLPLQTWLADAMAGPTPVSALIHAATMVTAGVYLIARTHGLFLMT
                     PEVLHLVGIVGAVTLLLAGFAALVQTDIKRVLAYSTMSQIGYMFLALGVQAWDAAIFH
                     LMTHAFFKALLFLASGSVILACHHEQNIFKMGGLRKSIPLVYLCFLVGGAALSALPLV
                     TAGFFSKDEILAGAMANGHINLMVAGLVGAFMTSLYTFRMIFIVFHGKEQIHAHAVKG
                     VTHSLPLIVLLILSTFVGALIVPPLQGVLPQTTELAHGSMLTLEITSGVVAVVGILLA
                     AWLWLGKRTLVTSIANSAPGRLLGTWWYNAWGFDWLYDKVFVKPFLGIAWLLKRDPLN
                     SMMNIPAVLSRFAGKGLLLSENGYLRWYVASMSIGAVVVLALLMVLR"
     gene            complement(5663..5965)
                     /gene="nuoK"
                     /locus_tag="NBI74_003150"
     CDS             complement(5663..5965)
                     /gene="nuoK"
                     /locus_tag="NBI74_003150"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HCD1933437.1"
                     /translation="MIPLQHGLILAAILFVLGLTGLVIRRNLLFMLIGLEIMINASAL
                     AFVVAGSYWGQTDGQVMYILAISLAAAEASIGLALLLQLHRRRQNLNIDSVSEMRG"
     gene            complement(5962..6516)
                     /gene="nuoJ"
                     /locus_tag="NBI74_003151"
     CDS             complement(5962..6516)
                     /gene="nuoJ"
                     /locus_tag="NBI74_003151"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HCD1933438.1"
                     /translation="MEFAFYICGLIAILATLRVITHTNPVHALLYLIISLLAISGVFF
                     SLGAYFAGALEIIVYAGAIMVLFVFVVMMLNLGGSEIEQERQWLKPQVWIGPAILSAI
                     MLVVIVYAILGVNDQGIDGTPISAKAVGITLFGPYVLAVELASMLLLAGLVVAFHVGR
                     EERAGEVLSNRKDDSAKRKTEEHA"
     gene            complement(6528..7070)
                     /gene="nuoI"
                     /locus_tag="NBI74_003152"
     CDS             complement(6528..7070)
                     /gene="nuoI"
                     /locus_tag="NBI74_003152"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015959823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HCD1933439.1"
                     /translation="MTLKELLVGFGTQVRSIWMIGLHAFAKRETRMYPEEPVYLPPRY
                     RGRIVLTRDPDGEERCVACNLCAVACPVGCISLQKAETKDGRWYPEFFRINFSRCIFC
                     GLCEEACPTTAIQLTPDFEMGEYKRQDLVYEKEDLLISGPGKYPEYNFYRMAGMAIDG
                     KDKGEAENEAKPIDVKSLLP"
     gene            complement(7085..8062)
                     /gene="nuoH"
                     /locus_tag="NBI74_003153"
     CDS             complement(7085..8062)
                     /gene="nuoH"
                     /locus_tag="NBI74_003153"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015875065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HCD1933440.1"
                     /translation="MSWISPELIEILLTVLKAVVILLVVVTCGAFMSFGERRLLGLFQ
                     NRYGPNRVGWGGSLQLVADMIKMFFKEDWIPKFSDRVIFTLAPMIAFTSLLLAFAIVP
                     VSPGWVVADLNIGILFFLMMAGLAVYAVLFAGWSSNNKYSLLGAMRASAQTLSYEVFL
                     GLSLMGVVAQAGSFNMTDIVNSQAHVWNVIPQFFGFITFAIAGVAVCHRHPFDQPEAE
                     QELADGYHIEYSGMKFGLFFVGEYIGIVTISALMVTLFFGGWQGPLLPPFIWFALKTA
                     FFMMMFILIRASLPRPRYDQVMSFGWKICLPLTLINLLVTAAVILWQAQ"
     gene            complement(8059..10785)
                     /gene="nuoG"
                     /locus_tag="NBI74_003154"
     CDS             complement(8059..10785)
                     /gene="nuoG"
                     /locus_tag="NBI74_003154"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311194.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HCD1933441.1"
                     /translation="MATIHVDGKEYEVNGADNLLEACLSLGLDIPYFCWHPALGSVGA
                     CRQCAVKQYQNAEDTRGRLVMSCMTPASDGTFISIDDEEAKQFRESVVEWLMTNHPHD
                     CPVCEEGGNCHLQDMTVMTGHSFRRYRFTKRTHRNQDLGPFISHEMNRCIACYRCVRY
                     YKDYADGTDLGVYGAHDNVYFGRPEDGTLESEFSGNLVEICPTGVFTDKTHSERYNRK
                     WDMQFAPSICQQCSIGCNISPGERYGELRRIENRYNGTVNHYFLCDRGRFGYGYVNLK
                     DRPRQPVQRRGDDFITLNAEQAMQGAADILRQSKKVIGIGSPRASVESNFALRELVGE
                     ENFYTGIAHGEQERLQLALKVLREGGIYTPALREIESYDAVLVLGEDVTQTGARVALA
                     VRQAVKGKAREMAAAQKVADWQIAAILNIGQRAKHPLFVTNVDDTRLDDIAAWTYRAP
                     VEDQARLGFAIAHALDNSAPAVDGIEPELQSKIDVIVQALAGAKKPLIISGTNAGSAE
                     VIQAAANVAKALKGRGADVGITMIARSVNSMGLGIMGGGSLEEALTELETGRADAVVV
                     LENDLHRHASATRVNAALAKAPLVMVVDHQRTAIMENAHLVLSAASFAESDGTVINNE
                     GRAQRFFQVYDPAYYDSKTVMLESWRWLHSLHSTLLSREVDWTQLDHVIDAVVAKIPE
                     LAGIKDAAPDATFRIRGQKLAREPHRYSGRTAMRANISVHEPRQPQDIDTMFTFSMEG
                     NNQPTAHRSQVPFAWAPGWNSPQAWNKFQDEVGGKLRFGDPGVRLFETSENGLDYFTS
                     VPARFQPQDGKWRIAPYYHLFGSDELSQRAPVFQSRMPQPYIKLNPADAAKLGVNAGT
                     RVSFSYDGNTVTLPVEIAEGLTAGQVGLPMGMSGIAPVLAGAHLEDLKEAQQ"
     gene            complement(10838..12175)
                     /gene="nuoF"
                     /locus_tag="NBI74_003155"
     CDS             complement(10838..12175)
                     /gene="nuoF"
                     /locus_tag="NBI74_003155"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HCD1933442.1"
                     /translation="MKNIIRTPETHPLTWRLRDDKQPVWLDEYRSKNGYEGARKALTG
                     LSPDEIVNQVKDSGLKGRGGAGFSTGLKWSLMPKDESMNIRYLLCNADEMEPGTYKDR
                     LLMEQLPHLLVEGMLISAFALKAYRGYIFLRGEYIEAAVNLRRAIAEATEAGLLGKNI
                     MGTGFDFELFVHTGAGRYICGEETALINSLEGRRANPRSKPPFPATSGAWGKPTCVNN
                     VETLCNVPAILANGVEWYQNISKSKDAGTKLMGFSGRVKNPGLWELPFGTTAREILED
                     YAGGMRDGLKFKAWQPGGAGTDFLTEAHLDLPMEFESIGKAGSRLGTALAMAVDHEIN
                     MVSLVRNLEEFFARESCGWCTPCRDGLPWSVKILRALERGEGQPGDIETLEQLCRFLG
                     PGKTFCAHAPGAVEPLQSAIKYFREEFEAGIKQPFSNTHLINGIQPNLLKERW"
     gene            complement(12172..12672)
                     /gene="nuoE"
                     /locus_tag="NBI74_003156"
     CDS             complement(12172..12672)
                     /gene="nuoE"
                     /locus_tag="NBI74_003156"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HCD1933443.1"
                     /translation="MHENQQPQTEAFELSAAEREAIEHEMHHYEDPRAASIEALKIVQ
                     KQRGWVPDGAIHAIADVLGIPASDVEGVATFYSQIFRQPVGRHVIRYCDSVVCHINGY
                     QGIQAALEKKLNIKPGQTTFDGRFTLLPTCCLGNCDKGPNMMIDEDTHAHLTPEAIPE
                     LLERYK"
     gene            complement(12675..14477)
                     /gene="nuoC"
                     /locus_tag="NBI74_003157"
     CDS             complement(12675..14477)
                     /gene="nuoC"
                     /locus_tag="NBI74_003157"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C/D"
                     /protein_id="HCD1933444.1"
                     /translation="MVNNMTDLTAQEPAWQTRDHLDDPVIGELRNRFGPDAFTVQATR
                     TGVPVVWIKREQLLEVGDFLKKLPKPYVMLFDLHGMDERLRTHREGLPAADFSVFYHL
                     ISIDRNRDIMLKVALAENDLHVPTFTKLFPNANWYERETWDLFGITFDGHPNLRRIMM
                     PQTWKGHPLRKDYPARATEFSPFELTKAKQDLEMEALTFKPEEWGMKRGTENEDFMFL
                     NLGPNHPSAHGAFRIVLQLDGEEIVDCVPDIGYHHRGAEKMGERQSWHSYIPYTDRIE
                     YLGGCVNEMPYVLAVEKLAGITVPDRVNVIRVMLSELFRINSHLLYISTFIQDVGAMT
                     PVFFAFTDRQKIYDLVEAITGFRMHPAWFRIGGVAHDLPRGWDRLLREFLDWMPKRLA
                     SYEKAALQNTILKGRSQGVAAYGAKEALEWGTTGAGLRATGIDFDVRKARPYSGYENF
                     DFEIPVGGGVSDCYTRVMLKVEELRQSLRILEQCLNNMPEGPFKADHPLTTPPPKERT
                     LQHIETLITHFLQVSWGPVMPANESFQMVEATKGINSYYLTSDGSTMSYRTRIRTPSY
                     AHLQQIPAAIRGSLVSDLIVYLGSIDFVMSDVDR"
     gene            complement(14571..15233)
                     /gene="nuoB"
                     /locus_tag="NBI74_003158"
     CDS             complement(14571..15233)
                     /gene="nuoB"
                     /locus_tag="NBI74_003158"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoB"
                     /protein_id="HCD1933445.1"
                     /translation="MDYTLTRIDPNGENDRYPLQKQEIVTDPLEQEVNKNVFMGKLND
                     MVNWGRKNSIWPYNFGLSCCYVEMVTSFTAVHDVARFGAEVLRASPRQADLMVVAGTC
                     FTKMAPVIQRLYDQMLEPKWVISMGACANSGGMYDIYSVVQGVDKFIPVDVYIPGCPP
                     RPEAYMQALMLLQESIGKERRPLSWVVGDQGVYRANMQSERERKRGERIAVTNLRTPD
                     EI"
     gene            complement(15249..15692)
                     /gene="nuoA"
                     /locus_tag="NBI74_003159"
     CDS             complement(15249..15692)
                     /gene="nuoA"
                     /locus_tag="NBI74_003159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoA"
                     /protein_id="HCD1933446.1"
                     /translation="MSMSTSTEVIAHHWAFAIFLIVAIGLCCLMLVGGWFLGGRARAR
                     SKNVPFESGIDSVGSARLRLSAKFYLVAMFFVIFDVEALYLFAWSTSIRESGWVGFVE
                     AAIFIFVLLAGLVYLVRIGALDWTPARSRRERMNPETDSIANRQR"
     gene            complement(16322..17260)
                     /gene="lrhA"
                     /locus_tag="NBI74_003160"
     CDS             complement(16322..17260)
                     /gene="lrhA"
                     /locus_tag="NBI74_003160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LrhA"
                     /protein_id="HCD1933447.1"
                     /translation="MISANRPIINLDLDLLRTFVAVADLNTFAAAAAAVCRTQSAVSQ
                     QMQRLEQLVGKELFARHGRNKLLTEHGIQLLGYARKILRFNDEACSSLMFSNLQGVLT
                     IGASDESADTILPFLLNRVSSVYPKLALDVRVKRNAYMAEMLESQEVDLMVTTHRPST
                     FKALNLRTSPTHWYCAAEYVLQKGEPIPLVLLDDPSPFRDMVLATLNKADIPWRLAYV
                     ASTLPAVRAAVKAGLGVTARPVEMMSPDLRVLSGVDGLPPLPDTEYLLCYDPSSNNEL
                     AQVIYQTMESYHNPWQYSPMSAPEGDDSLLIERDIE"
BASE COUNT         4422 a         4935 c         4590 g         3752 t
ORIGIN      
        1 atttctcgag gcggatccag aaaaagcaac atgcgcattt tattttcttt cgtctgtttt
       61 aagatgcgaa acatgaaatt caacggagga aaaataaatg ggaatgattg gctattttgc
      121 agaaattgac tcagaaaaga ttaaccaact gcttgagtct actaaaaaac ctttgatgga
      181 taatatacat gacacgcttt caggattacg ccgactggat attgataaac gctgggattt
      241 tttacacttt ggcctgacgg gtacatctgc atttgacccc gcgaagaatg acccgctaag
      301 tcgcgcagtg cttggtgaac acagcctaga agatggcata gatggcttcc tgggattgac
      361 ctggaatcag gagttagccg cgacaatcga ccgactggaa tcactggatc gaagtgagct
      421 gcgtaaacag ttttccatta agcgactcaa tgaaatggag atttatccgg gagtaacctt
      481 ttcagaagag ttagaggggc aactctttgc ttcgatcatg cttgatatgg aaaagctgat
      541 tagcgcgtat cgacgtatgc tgcgacaagg gaatcatgcg ctgacggtta ttgttgggtg
      601 atggtgtcag ggcacccaaa aaaaccgccg aacatgtcgg cggctttctg acttacaaac
      661 tgactgctta catcagcggc attgctaacc gcacaatgct aatcagcggt tgtggccata
      721 cacccagcac cagtaccaac agtgcagaga tcagcaccac gataccgcct gcgctgtact
      781 gccagtttga tggtgcatcg cgacccggtt gttccggagc gtgaaggtat aagctcaccg
      841 ccacacgcag gtagtagtag aggccgattg ccgaaccgac aactacggca cccaccagcc
      901 accacaagtg tgcctggaca ccgaccgcca gcacgtagaa cttaccgata aagcccagcg
      961 tcattgggat accagccagt gaaagcatca tcaccgtcat cactgccgcg aggatcggac
     1021 gatgccagaa cagaccgcgg taagagaaca gggaatcagc atccgggcca cgatacgggc
     1081 tggacatcag gctgaccacg ccgaacgcgc cgaggctgct gaacagataa cctgccaggt
     1141 aaacccctac cgcttccatc gacatctcgc cggtttgcag cgcaatcagc gccaccagca
     1201 gatagccgag gtgagagata gatgagtaac cgagcagacg tttgatattg gtctggctca
     1261 gcgccatcag gttaccgaag atgatggagg caaaggcgat aatcgccagc actacgcgaa
     1321 tcgcttcact gtcacccacc ggtgcgtaaa ggaacaaacg catcaccacg ccgaagatag
     1381 cgattttgct cgccgtcgcc aggaaagtgg aaaccggcgc aggcgcgccc tggtatacgt
     1441 ctggcgtcca caggtggaac ggcaccagag agagtttgaa gccgaggcca acaatcatca
     1501 ggccgaaacc tgccagcaac agcggctcgt tgagcatacc gtcgccgagg tttttaccca
     1561 gtgcgacaaa cgacaggtcg ccagactgcg catacaccag cgccataccg aacagcagga
     1621 aagaagacgc tgcggcagaa aggatggtgt atttgatact ggcttccagc gaacgtttct
     1681 ggcggaaagc gtaaccgacc aggccaaaca gcggcaaaga gatcagttcg ataccgagga
     1741 acagagacgc cagatggttg gcattcgcca gcaggatccc gcccagcgcg gcaattaaca
     1801 ccaacaggta gaactcatcc ttgttgtcgt tatagccttc aagccacggg taggcgaaag
     1861 tacaggtggc gaggctcgcc aacaatacca gcccggtgta aagcatggcg aaaccatcaa
     1921 cgcgcatcag tggcgtaacg tccatagcgc ccgcctggcc gacaaaccag agcgaaacca
     1981 gcgccgcgtt aagcccaata accgagagcg tagcgttgag aaaatgattg cgtcgccacg
     2041 caatggagag catcacaacc accaccgtca agccgacgat cagcaacggt agcagtgcga
     2101 tcaggttttg tggagttatt gtcatggcga tttacggcct tgtagtagta acggaattaa
     2161 caaaccactg ctggatattg ccaatcgcgg agtgcgaggt atccagaatc ggctgcggat
     2221 agaagcccag cagtaccagc agcaccacca gcaacaggat cataaacagc tcacgcagcg
     2281 acatccctgg cagttcctgg ctggcaatct ggcttttcgc tttaccgaaa taagcgcgat
     2341 gcagcatcgc cagcgaataa acagatgcaa agaccagccc aaaggtagag ataacggtaa
     2401 ttaccgggac aacctggaag ctaccgaaca gaatcataaa ttcgccgacg aagttaccgg
     2461 tgccaggcat cccaagcgtt gccaccgcaa agaacagcga cagtgctggc agccacttca
     2521 tcttgctcca cagaccgccc atcatgcgca tgtcgcgggt atggatacgt tcatagagct
     2581 gaccgcagag aataaacaga cccgccgctg acaagccgtg tgcaatcatc tggattaccg
     2641 cgccctggta ggccaactgg ctaccggtgt agatagcaat cagcacgaag cccatgtggg
     2701 aaaccgaggt gtaggcgatc agacgtttga tatcggtctg ggcgaaggcc atccacgcac
     2761 catagaagat gccgataaca cccagccaca tagcgattgg cgcgaactct gccgacgcgt
     2821 tcgggaacag cggtagggag aaacgcagca gaccgtaagc ggcggttttc agcaagatcc
     2881 ccgcgaggtc aacggaaccg gcggtcggag cctgggagtg cgcatccggc agccagccat
     2941 gcagcggaac caccggcatt ttcactgcga aggcgatgaa gaagcccagc atcaacaggt
     3001 attccacgcc gttagacatc ggcgtattca gcagctcttc atagttgaag gtccagacgc
     3061 cggtcgcatt gtagtgaacg aaaaccagcg cctggatggc gatcaacatc accagaccac
     3121 tcgcctgggt gtaaatgaag aacttggttg ccgccgtgat acgcgtttta ccgtcagagg
     3181 ctttatgccc ccacagtgcg atcaggaagt acatcggcac cagcatcatt tcccagaaga
     3241 agaagaacag gaacatgtcg atggcaagga acacgccgat aacgccgccc aggatccaca
     3301 tcaggttgag gtggaagaag ccctggtatt tttcgatctc tttccacgaa cagagtaccg
     3361 ccagcacacc gagcagaccg gtcagcacga ccatcagcaa cgacagaccg tcaatggcga
     3421 gatggataga gataccaaaa cgcgggatcc acggcatgtc gaattcagac tgccactgcg
     3481 gaattccggc ggattgcgtc agtgaataac cgccctgcaa ccacagttgc agcgacagcg
     3541 ccagcgtcaa tcccatggtt accagcgcga tccagcgcgg caccttgacg ccaaagcgtt
     3601 cggtctgcca gcacaggaag ccgccgataa agggaattaa tattagccag ggtagtaaca
     3661 tggcgatctt tattccttgt aaaagtcccg tcaggaccgg attttcaacg aattctcacg
     3721 acagaaaatt ctcttcggac tgggggtagt gtcggatgcg ccgcgaagac ggcttatccg
     3781 acctacgttc ttatatgccc aggggcaatc ccacaatcct taactcaacg cagtaccatc
     3841 aacagagcca gcaccacgac cgcaccgatg ctcatggatg ccacatacca gcgcagataa
     3901 ccgttctcgc ttaacagcag acctttacct gcaaagcggg aaaggacagc cgggatgttc
     3961 atcattgagt tcagcggatc gcgtttcagc aaccaggcaa tacccaggaa cggcttgacg
     4021 aacactttgt cgtacagcca gtcaaagccc caggcgttgt accaccaggt acccagcaga
     4081 cggcccggcg cactgttggc gatggaggtc accagagtac gtttacccag ccacagccag
     4141 gcagccagca gaatgccgac caccgcgacc acgccagagg taatttccag ggtcaacatg
     4201 ctgccgtgcg ccagctccgt cgtttgcgga agcacgccct gcagcggcgg tacaatcagt
     4261 gcaccaacga aggtggaaag gatcagcagc acaatcagcg gcaggctgtg agttacccct
     4321 ttcacggcgt gagcgtgaat ttgttctttt ccgtggaaga cgatgaaaat catacggaag
     4381 gtgtagagcg aggtcataaa cgcaccgacc agacctgcca ccatcagatt gatatgtcca
     4441 ttcgccatcg cacccgcgag gatctcatcc ttactgaaga agcccgcagt gaccagcggc
     4501 agtgccgata gtgctgcgcc gcccaccagg aagcagagat aaaccagcgg aatagattta
     4561 cgcagaccac ccatcttaaa gatgttctgt tcgtgatggc aggccagaat gacggaaccg
     4621 gatgccagga acagcagcgc tttaaagaac gcgtgggtca tcaagtggaa aatcgccgca
     4681 tcccatgcct gcacgccaag cgcgaggaac atgtagccaa tctggctcat ggtagagtaa
     4741 gcgagaacac gtttgatgtc ggtctgtacc agcgcggcaa aaccggccag cagcagcgta
     4801 accgccccga caatacccac cagatgcaga acttccggcg tcatcaggaa caggccgtgg
     4861 gtacgggcga tcaggtagac acccgcggtt accattgttg cggcgtggat cagcgcggag
     4921 acaggcgtcg ggcccgccat cgcgtcggca agccatgtct gcaacggcaa ctgcgcagat
     4981 ttaccgaccg caccgcccag cagcatcagc gtcgcccaca tcagcatgtt attgccgtca
     5041 gcaaagtgcg ctggtgccag ttccaccatt tcgcggaagt tcagggtgcc cagttcgttg
     5101 taaagaatga acagcgcaaa agcgaggaag acgtcaccca cacgggtcac gacgaacgct
     5161 ttcattgccg ctgcgccatt cttcggatcg gtgtaataga aaccgatcag cagataggag
     5221 cacaggccca cgccttccca gccgaggtac atcaacagta ggttatcggc gagaaccaga
     5281 actaccatgc tggcgatgaa caggttggtg taagcgaaga agcgagagta gccctcttct
     5341 ccgcgcatat accaggaggc atacatgtga ataaggaaac ccacaccagt gaccactgag
     5401 agcatggtca gcgacaggcc gtccagcacc agattgaaac cgatgttaaa gtcgcctacc
     5461 gacatccacg tccacagcgg ctggctatat gcctgctcac cgttagcgaa gaagtcaacg
     5521 ccgataaagg cggttaccag cgccgccagg cccacagagc caacgccgac gatcgccgag
     5581 acgttttcag accagcgccc acgggagaac gccagcagga cgaagccaat caatggcaaa
     5641 ataatggtta aggcaagcat gttcatccgc gcatctcact tactgaatcg atgttcaggt
     5701 tctggcgacg acggtgaagt tgcagcagca gcgcaaggcc gatactcgct tccgccgccg
     5761 cgaggctgat ggcgagaatg tacatcacct gaccgtcggt ctggccccag tagcttccgg
     5821 cgaccacaaa ggccagcgcg gaggcgttaa tcatgatttc cagaccaatc agcataaaca
     5881 gcagattgcg acggataacc agaccggtta agccaagaac gaataagatt gccgcgagga
     5941 tcagtccatg ttgtaagggg atcatgcgtg ctcctccgtt tttcttttcg cgctgtcgtc
     6001 tttacgattg ctcagcactt cacccgcacg ctcttcacga ccgacgtgga aggccacaac
     6061 cagacccgcg agcagcagca tagaagccag ttccaccgcc agtacgtaag gcccgaacag
     6121 cgtaataccc actgctttag cactgattgg cgtaccgtcg ataccttgat cgttaacacc
     6181 gagaatggcg taaacaatca ccaccagcat gatggctgac aaaattgccg gaccaatcca
     6241 cacctgcggt ttcaaccact ggcgttcctg ttcgatttct gaaccgccca ggttgagcat
     6301 catcaccacg aacacgaaca gcaccataat ggcacccgca tagacgataa tttccagcgc
     6361 accggcaaag taagcgccca gtgagaagaa caccccagag atcgccagca gcgaaataat
     6421 caggtacagc agcgcgtgta ccggattggt atgggtgatc actcgcaagg tcgcaagtat
     6481 ggctatcagg ccacagatat aaaaagcgaa ctccattgcc cctctcctta cggtaacagg
     6541 ctcttgacgt cgataggctt ggcttcgttc tctgcttcgc ccttatcttt gccgtcgatt
     6601 gccatacctg ccatccggta gaagttatat tccgggtatt tgcccggacc ggagatcagc
     6661 agatcctctt tctcgtaaac cagatcctgg cgcttgtatt cccccatttc gaaatccggg
     6721 gttaactgaa tcgccgtggt cggacaggct tcttcgcaca gaccacagaa aatgcagcgt
     6781 gagaagttga tgcggaaaaa ttccggatac cagcgaccgt ctttggtttc tgctttttgc
     6841 agcgagatac agccgaccgg gcaggctacc gcgcagaggt tacaggctac gcaacgctct
     6901 tcgccgtccg gatcgcgggt cagaacgata cgaccacgat aacggggcgg cagatagacc
     6961 ggctcttccg ggtacattcg cgtttcgcgt ttggcgaacg cgtgcaggcc gatcatccag
     7021 atactacgaa cctgggtgcc gaaacctact aacaattctt ttaaggtcat ggtcttattg
     7081 ccccttattg cgcctgccag agaatgacag ccgccgttac cagcaagttg atcagcgtca
     7141 gcggcaggca gattttccag ccgaaggaca ttacctggtc ataacgcgga cgcggtaacg
     7201 acgcacgaat caaaatgaac atcatcataa agaacgcggt tttcagcgcg aaccagatga
     7261 atggcggtaa caacgggcct tgccagccac cgaagaacag cgtcaccatc agtgcagaga
     7321 tggtcacaat cccgatgtat tcgcccacga agaacagacc gaacttcata ccggaatatt
     7381 caatgtggta accatccgcc agttcctgct cggcttccgg ctggtcaaac gggtgacggt
     7441 gacacaccgc cacgcccgcg atggcaaagg taataaaacc aaagaattgc gggatgacgt
     7501 tccacacatg cgcctggctg ttgacgatgt cggtcatgtt gaatgaaccg gcctgcgcca
     7561 ccacgcccat caaggaaagc ccgaggaaca cttcgtagct cagggtctgc gcagaagcac
     7621 gcatcgcacc cagcagcgag tatttgttgt tacttgacca gcccgcaaac agcaccgcgt
     7681 aaaccgccag acctgccatc atcaggaaga acaaaatccc gatgttcagg tcggcaacca
     7741 cccaacccgg actgactggc acaattgcaa aggccagcag cagcgaggta aaggcaatca
     7801 tcggtgccag ggtaaagatg acgcgatccg agaatttcgg gatccagtct tctttaaaga
     7861 acattttgat catgtccgca accagctgga gcgaaccgcc ccagccaaca cggttaggtc
     7921 cataacggtt ctggaacaga cccagcaggc gacgttcgcc aaagctcatg aatgccccac
     7981 aggtgactac caccagcagg atcaccaccg ctttaaggac ggtcagcagg atctcaatca
     8041 gttccggtga tatccaactc attgttgtgc ctccttgaga tcctcaagat gcgcgccagc
     8101 cagcaccgga gcaatgccgg acatacccat cggtaagccc acctgccctg ccgtcagccc
     8161 ttcggcgatt tcaaccggca gcgtgaccgt gttgccatcg taactaaagg agacgcgtgt
     8221 acctgcgttc acccccaact tcgcggcatc cgctgggttg agtttgatgt acggctgcgg
     8281 catacggctc tggaagaccg gagcacgctg tgacaattca tcgctgccaa acaggtggta
     8341 gtacggcgcg atacgccatt tcccgtcctg cggctggaag cgtgccggta cgctggtgaa
     8401 gtaatccaga ccgttttcgc tggtttcaaa cagacgcacg cccggatcgc caaaacgcag
     8461 tttgccgccc acttcgtcct ggaatttgtt ccacgcctgc ggggagttcc agcctggtgc
     8521 ccaggcaaac ggcacttgcg aacggtgcgc ggtcggctgg ttgttacctt ccatcgagaa
     8581 ggtgaacata gtgtcaatat cctgcggctg acgcggctca tgaacgctga tattggcgcg
     8641 catggcggta cgaccgctat agcgatgcgg ttcacgggcc agtttctgcc cacgaatacg
     8701 gaatgtcgca tccggcgcag catctttgat acctgccagt tccgggattt tcgccacaac
     8761 agcgtcaatc acgtggtcga gctgcgtcca gtccacttca cggctcagca gggtgctatg
     8821 cagcgagtgc agccagcgcc agctttccag catgacagtt ttgctgtcgt aataagccgg
     8881 atcgtaaacc tggaagaaac gttgggcgcg gccttcgttg ttgatcaccg taccgtcgct
     8941 ttcagcaaag ctggcggcgg aaagtaccag atgggcgttt tccataatcg ctgtgcgttg
     9001 atggtcaacc accatcacca gcggcgcttt agccagcgca gcattcacgc gggtagcgga
     9061 agcgtgacga tgcagatcgt tttccagcac caccaccgcg tcggcgcgtc cggtttccag
     9121 ttcggttaac gcttcttcaa gcgatccacc gcccataatg cccagcccca tgctgttgac
     9181 ggaacgggca atcatggtga taccgacgtc agcgccgcga cctttcaggg ctttcgccac
     9241 gttagccgca gcctgaatca cttcagcgct accagcgttc gtcccggaga taatcaacgg
     9301 tttcttcgca ccagccagtg cctgcacgat gacgtcgatt ttgctttgca gctcaggctc
     9361 aataccgtca accgctggtg cagagttatc cagcgcatga gcgatggcaa aacctaaacg
     9421 cgcctgatct tcaaccggtg cgcggtaagt ccacgccgcg atatcatcca gacgggtgtc
     9481 atcaacgttg gtaacaaaca gcggatgctt cgcacgttga ccgatgttga ggattgccgc
     9541 aatctgccag tcagccactt tctgtgctgc cgccatttcg cgcgctttac ctttcaccgc
     9601 ctgacgcact gccagcgcga cgcgcgcgcc ggtctgggta acgtcttcgc ccagcaccag
     9661 taccgcatcg taagattcga tttcgcgcag agccggagta taaatgccgc cttcacgcag
     9721 tactttcagc gccagttgca gacgttcctg ctcaccgtga gcgataccgg tatagaagtt
     9781 ttcttcgccc accagctcac gcagcgcaaa gttgctttcc acgctggcac gcggagaacc
     9841 aataccgatc actttcttcg actgacgcag aatatctgct gcgccctgca ttgcctgttc
     9901 ggcgttgagg gtaatgaaat catcgccacg acgctgtact ggctgacgcg gacgatcttt
     9961 caggttgacg taaccgtaac cgaaacgacc acggtcgcag aggaagtagt ggtttaccgt
    10021 accgttgtaa cggttttcga tacgacgcag ttcgccataa cgctcgccgg ggctgatgtt
    10081 acagccgatg gaacattgct ggcagatgct cggcgcaaac tgcatatccc atttacggtt
    10141 gtaacgctcg gagtgcgttt tgtcggtgaa tacgccggtc ggacaaattt cgaccaggtt
    10201 accggaaaat tcgctttcca gcgtgccgtc ttccgggcga ccgaagtaga cgttgtcgtg
    10261 cgcaccgtaa acgcccaggt ccgtaccgtc agcgtaatct ttgtagtaac gcacgcagcg
    10321 gtagcaggcg atgcagcggt tcatttcgtg agagatgaac ggccccaaat cctgattacg
    10381 gtgagtacgt ttggtgaaac ggtagcgacg gaagctgtgt ccggtcatca cggtcatatc
    10441 ctgaaggtgg cagttaccgc cctcttcaca taccggacag tcgtgcgggt ggttggtcat
    10501 caaccactcg accacgcttt cacggaactg tttcgcttct tcgtcgtcaa tggaaataaa
    10561 ggtgccatcg gaagccggtg tcatacagga catcaccagg cgaccacgcg tgtcttccgc
    10621 gttttggtat tgcttcaccg cacactggcg gcaagcaccg acgcttccca gcgccggatg
    10681 ccagcaaaag taaggaatat caaggcccag agacagacaa gcttccagca ggttgtccgc
    10741 cccgttgacc tcgtattctt tgccgtctac atgaattgta gccattagca tgcttccagt
    10801 tttctcagtc agagactgag cgttaatcga aattcggtta ccagcgctct ttcagcaggt
    10861 tcggctgaat cccattaatc aaatgggtat tgctgaacgg ctgtttgatt cccgcctcaa
    10921 attcttcgcg gaaatatttg atggcgctct gtaacggctc cactgcacca ggtgcgtggg
    10981 cacagaaagt tttacccgga cctaagaatc gacacagttg ctcaagtgtt tcgatatcgc
    11041 ccggctgacc ttcaccacgc tccagcgcac gcagaatttt cacgctccac ggcagaccgt
    11101 cgcggcatgg cgtacaccag ccgcaggact cacgggcgaa aaactcttcc aggttacgca
    11161 ccagcgacac catgttaatc tcgtggtcaa ccgccatcgc cagcgccgta cccagacggc
    11221 ttcccgcttt accgatactt tcgaattcca tcggcaggtc aaggtgcgct tcggtcagga
    11281 agtcggtgcc cgcgccgcct ggctgccagg ctttgaattt caggccgtca cgcataccac
    11341 cagcgtaatc ttcaaggatt tcacgcgcgg tggtgccgaa cggcagttcc cacagacccg
    11401 gatttttcac ccgaccggag aagcccatca gcttggtgcc agcatcttta cttttcgaga
    11461 tgttctgata ccactccacg ccgttagcga ggatcgccgg aacgttacac agggtttcga
    11521 cgttgttaac gcaggttggt ttaccccatg caccggaggt tgccgggaag ggcggcttcg
    11581 agcgtgggtt agcgcgacgc ccttccaggg agttgattaa cgctgtttct tcaccgcaga
    11641 tgtagcgccc tgccccggta tggacgaaca gttcgaaatc gaaacctgtt cccataatgt
    11701 ttttgccaag cagacccgct tcggtggctt cggcaatggc acggcgcaga ttcactgccg
    11761 cttcaatata ttcgccacgc aagaagatgt agccacgata cgctttcagc gcaaacgcag
    11821 aaatgagcat accttccacc agcaggtgcg gtagttgctc catcagcaga cggtctttat
    11881 aggtgcccgg ctccatttca tcggcattac acagcaggta acggatgttc atggattcgt
    11941 ctttcggcat caggctccac ttcaggccag tagaaaagcc cgcgccgccg cgccctttca
    12001 gaccagagtc ttttacctga ttaacgattt cgtccggaga cagcccggtc agcgccttac
    12061 gcgcgccttc gtaaccgttt ttgctgcgat attcgtccag ccacactggc tgtttgtcat
    12121 cgcgcagacg ccaggtcagc ggatgcgttt cgggagtacg gataatgttt ttcatttata
    12181 ccgctccagc agttcaggga tcgcttccgg ggtcagatgc gcgtgagtgt cctcatcgat
    12241 catcatgttt ggccctttat cacagttccc caggcagcaa gttggcagca gcgtaaagcg
    12301 accgtcaaat gtcgtttgcc ctggtttgat gttcagtttt ttctccagcg ccgcctgaat
    12361 accctggtaa ccgttgatat gacagaccac gctgtcgcaa taacggatca catggcggcc
    12421 tactggctga cggaagatct ggctataaaa cgttgccacg ccttcgacgt cgctcgccgg
    12481 aatacccagc acatcggcga tcgcgtggat cgcgccatcc ggcacccagc cacgctgctt
    12541 ctgaacgatt ttcagcgctt caatggacgc cgcacgcggg tcttcgtagt ggtgcatctc
    12601 gtgctcaatc gcttcacgct ctgccgcact cagctcaaaa gcctcggttt gtggttgttg
    12661 attctcgtgc ataattagcg gtccacatct gacataacaa aatcgatact gcccagataa
    12721 acaatcaggt cagacaccag gctgccgcgg atcgccgccg gaatttgctg caaatgcgca
    12781 taactcggcg tacggatacg ggtgcgataa ctcatggtgc tgccgtcgct ggtcaggtag
    12841 taactgttga tccccttggt cgcctcaacc atctggaagg attcattggc aggcatcacc
    12901 ggaccccacg acacttgcag gaagtgggtg atcagggttt cgatatgttg cagcgtgcgc
    12961 tctttcggcg gcggcgtggt cagcggatga tccgccttga acgggccttc cggcatgttg
    13021 ttgaggcact gctcaagaat gcgcagactc tggcgcagct cttccacttt cagcattacg
    13081 cgggtgtagc agtcagaaac accaccaccc accgggattt caaagtcgaa gttttcatag
    13141 ccagaataag gacgcgcctt acgcacgtcg aagtcgatcc cggtagcacg caggcccgcg
    13201 ccagtggtgc cccactccag cgcctctttc gcgccatagg cggcaacgcc ctgggaacga
    13261 cctttcagaa tggtgttttg cagcgccgct ttctcgtaag acgccagacg tttaggcatc
    13321 cagtcgagga actcacgcag caggcgatcc cagccgcgcg gcaggtcgtg cgctacgccg
    13381 ccaatacgga accacgccgg gtgcatacga aaaccagtga ttgcttccac cagatcgtaa
    13441 attttctgac gatcggtaaa ggcgaagaac actggcgtca tcgcgccgac gtcctgaata
    13501 aaggtggaga tatacagcag gtgactgttg atgcggaaca gttcggagag cattacgcga
    13561 atgacgttaa cgcgatccgg cacggtgatc ccagccagtt tctctaccgc cagcacataa
    13621 ggcatttcgt taacgcagcc gccgaggtat tcgatacggt cggtatacgg aatgtagctg
    13681 tgccaggact ggcgttcgcc cattttctca gcgccacggt ggtggtaacc gatgtctggt
    13741 acgcagtcga caatctcttc gccatcaagc tgcaaaacga tacggaaagc accgtgcgca
    13801 gacgggtggt tcggaccgag gttgaggaac atgaagtcct cgttttcggt gccgcgcttc
    13861 atcccccact cttccggttt gaaggtcagg gcttccatct ccagatcctg tttggctttg
    13921 gtcagctcaa acggcgagaa ttcggtagcg cgcgccgggt aatctttacg cagcgggtga
    13981 cctttccagg tttgcggcat catgatgcgg cgcaggttcg ggtgaccgtc gaatgtaatg
    14041 ccaaatagat cccaggtttc acgctcatac cagttagcgt tcgggaatag tttggtaaag
    14101 gtcggtacgt gcaggtcgtt ttctgccagc gccaccttca gcatgatgtc gcggttacga
    14161 tcgatagaaa tcagatggta gaaaacggaa aaatccgcgg caggtaaccc ttcgcggtgt
    14221 gtgcgcagac gttcgtccat gccgtgtaag tcaaacagca tgacgtaagg tttcggcagt
    14281 ttctttaaga aatcgcccac ttccagtaat tgttcacgct tgatccacac aacgggaacc
    14341 ccggtgcgag tcgcctgaac agtaaaggca tccggcccaa aacggttgcg cagttcgcca
    14401 atcaccggat catcaagatg atcgcgggtc tgccaggcgg gttcttgcgc ggttaagtcg
    14461 gtcatattgt tcaccattgc aaatggtccg tggtgactgt cggcgcagta agcttcgcga
    14521 aatagattta ggaataagcg aagaaaattc cctttgccga caggcgcaaa ttaaatctcg
    14581 tcaggtgtac gcaggttggt tacggcaatg cgttcaccgc gcttgcgttc gcgctctgat
    14641 tgcatattgg cgcgataaac gccctgatcg ccaaccaccc aggagagcgg acgacgttct
    14701 ttaccgatag attcctgcaa cagcatcagt gcctgcatgt acgcttcagg acgcggcgga
    14761 cagcccggga tatacacatc aaccgggatg aatttatcaa cgccctgcac aacggaataa
    14821 atatcgtaca taccgccgga gttggcacag gcacccattg agataaccca ttttggttcc
    14881 agcatctggt catacaaacg ctgaataacc ggtgccattt tggtaaagca ggttcctgca
    14941 accaccatca ggtcagcctg acgcggcgaa gcacgcaata cttcagcgcc aaaacgcgcc
    15001 acgtcatgca ccgcggtaaa cgaagtcacc atctcaacgt aacagcagga aaggccgaag
    15061 ttatacggcc aaattgagtt tttacgaccc cagttaacca tgtcattgag cttgcccata
    15121 aacacgtttt tgttaacttc ttgctccaga gggtcggtta cgatctcctg cttttgcagg
    15181 gggtaacggt cgttctcacc gttgggatct atgcgggtga gcgtataatc catcttaatg
    15241 cctcgcggtt agcgttgacg attagcgata ctgtccgttt ccgggttcat acgctcgcgg
    15301 cgtgaacgcg cgggcgtcca gtccagcgcg ccaatacgca ccagataaac cagacctgcc
    15361 agtaacacaa aaataaaaat tgcagcttcc acaaagccta cccagccgct ttcgcggata
    15421 gaggttgacc atgcgaacag atacagcgct tcaacgtcga agataacgaa gaacatggcc
    15481 accagataaa acttggcgga caggcgtaag cgggcggagc cgaccgagtc gataccggat
    15541 tcaaacggaa cgttttttga cctcgcgcgt gcgcgaccgc ctaaaaacca accgcctacc
    15601 agcatcaggc aacacaggcc aatggcaacg ataagaaaga tagcgaatgc ccagtgatga
    15661 gcgatgactt cagtggatgt tgacatactc attgcttact catcaaaagt agcgccagat
    15721 tcactgctct tcacggcaga tggacgccac atcgattcat ggggaggaat aaaaaaaacc
    15781 ttacaataac tgcagaaact attttataca gctaattgat gtggtctttt actcctttct
    15841 ataacctttt gtcaacttta acaaaggttt cttcacactg atttacacaa tgacaacaac
    15901 atttgcattt ggcgcccttt ctccccagat cattgacgac tccaggaaaa ttagatgttg
    15961 ttgaatcgtg tccgttgtga agcaatggaa aaaatacggg tctattttga caggaatttg
    16021 tgtcgattcc tccccccaaa agagagtatt ttcttgatct gtgacacgct tttgtcattc
    16081 tataacaaaa acgcagcaac aaattcacgt attttttaac attattgtag caggagattt
    16141 ttttcaggtg attatttgtg cgtacggggc gtgaatctct ggtgattgaa aaatgaacag
    16201 ttttatacgt tctgcacgat acgaggaagg ctttttacta aaaaaaagcc gctggggttt
    16261 aaaataaacc cagcggttcg tttttacact attgtctcag gaattatcta tcgtccgtcg
    16321 attactcgat atccctttca atcaacaatg agtcatctcc ttccggagca gacattgggc
    16381 tgtattgcca cggattatgg tagctctcca tcgtctgata gatcacctgg gccagctcat
    16441 tattactgga cgggtcatag cacagcaaat attcggtatc aggtagcggc ggtaagccat
    16501 ctactccact cagcacacgc aggtccggac tcatcatctc gaccggcctt gccgtcacgc
    16561 caagaccggc tttcactgcc gcacgaacgg ccggaagcgt cgaggcgaca taagccagtc
    16621 gccagggaat atctgcttta ttaagcgtcg ccagcaccat atcgcgaaac ggacttggat
    16681 catccagcaa cacaagaggg atcggctcac ctttttgcaa tacgtattcc gctgcgcagt
    16741 accagtgtgt tggcgaggta cgcaggttca atgccttaaa ggtagaagga cgatgggtgg
    16801 ttaccatcaa atccacttcc tgggattcca gcatttctgc catgtaggca ttacgtttaa
    16861 cgcggacatc cagcgctagt ttcggataaa ccgaactcac gcgatttaac aggaaaggta
    16921 agatcgtatc ggcagattca tctgaagcac cgatagttaa cacgccctga agattactga
    16981 acattaatga tgagcaggcc tcatcattaa aacgcaggat tttcctggcg taaccaagaa
    17041 gttgaatgcc atgttcagtt aacagtttgt tgcgaccgtg acgagcgaac agttctttcc
    17101 caacgagttg ttccagacgc tgcatttgct gacttacggc ggactgagta cgacacacag
    17161 cggcagctgc ggcagcaaaa gtgttcagat cggcaacagc aacaaatgtt ctcagcagat
    17221 cgaggtcgag gttaattatc ggacgatttg cacttatcat atattatcac ttactggcgg
    17281 ctcatactga gctggttaat gctgtgcaca cacaaacaag caattccatt tgtaatgtgc
    17341 ctccctggca gtttcatccg gaacccggac gaaagtaaaa atgcatatga gttgcactaa
    17401 aaaagcgact cacattgttc cgttataatg cctgaagtag atcacagaat atatcttcag
    17461 ggatcgcata tctattaagt tactcactct tttctattta tgacatgcgc gtgtttgtat
    17521 aaatgtaaat gtgagtcctt gttccactct cgtacagcat cgctggtcat acgcgaacac
    17581 gtacaaacag caatggtgag gcggcatcaa gagcagggat ccgtcattta tgcaagcatt
    17641 ttaccccaaa aaccttttat ttataagggt cattgcgaat tatctgatgc aaagttatg
//
LOCUS       DAHHTG010000092        17380 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.092, whole genome shotgun sequence.
ACCESSION   DAHHTG010000092 DAHHTG010000000
VERSION     DAHHTG010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17380)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17380)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17380
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.092"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            <1..1033
                     /locus_tag="NBI74_003161"
     CDS             <1..1033
                     /locus_tag="NBI74_003161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HCD1933448.1"
                     /translation="STLIQTKSNDVSIFYIFRQLSFICLTSLALFCYGKDNILDNNKK
                     KTGILLLALIPFLVFPLLAHNLSSYNADYSLYVVDYCPDNHTATWGINYTKILVCLWA
                     FLLFFIIMRTRLASELWPLIALLCLASLCCNLLLLTLDEYNYTIWYISRGIEVSSKLF
                     VVSFLIYNIFQELQLSSKLAVHNVLTNIYNRRYFFNSVESLLSRPVVKDFCVMLVDIN
                     QFKRINAQWGHRVGDKVLVSIVDIIQQSIRPDDILARLEGEVFGLLFTELNSAQAKII
                     AERMRKNVELLTGFSNRYDVPEQMTISIGTVFSTGDTHNISLVMTEADKALREAKSEG
                     GNKVIIHHI"
     gene            1214..2704
                     /locus_tag="NBI74_003162"
     CDS             1214..2704
                     /locus_tag="NBI74_003162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HCD1933449.1"
                     /translation="MKLHHRMLRHFIAASVIVLTSSFLIFELVASDRAMSAYLRYIVQ
                     RADSSFLYDKYQNQSIAAHVMRALAAEQSEVPPEQRRAICEAFESANNTHGLNLTAHK
                     YQGLRGTLQTASTDCDTIVEAAALLPAFDQAVEGNRHQDDYGSGLGMAEEKFHYYLDL
                     NDRYVYFYEPVNVEYFAMNNWSFLQSGSIGIDRKDIEKVFTGRTVLSSIYQDQRTKQN
                     VMSLLTPVYVAGQLKGIVLLDINKNNLRNIFYTHDRPLLWRFLNVTLTDTDSGRDIII
                     NQSEDNLFQYVSYVHDLPGGIRVSLSIDILYFITSSWKSVLFWILTALILLNMVRMHF
                     RLYQNVSRENISDAMTGLYNRKILTPELEQRLQKLVQSGSSVMFIAIDMDKLKQINDT
                     LGHQEGDLAITLLAQAIKQSIRKSDYAIRLGGDEFCIILVDSTPQIAAQLPERIEKRL
                     QHIAPQKEIGFSSGIYAMKENDTLHDAYKASDERLYVNKQNKNSRS"
     gene            2747..3250
                     /gene="yeaK"
                     /locus_tag="NBI74_003163"
     CDS             2747..3250
                     /gene="yeaK"
                     /locus_tag="NBI74_003163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mischarged aminoacyl-tRNA deacylase"
                     /protein_id="HCD1933450.1"
                     /translation="MTEMAKGSVTHQRLIALLSQEGANFRVVTHEAVGKCEAVSEIRG
                     TALGQGAKALVCKVKGNGVNQHVLAILAADQQADLSQLASHIGGLRASLASPAEVDEL
                     TGCVFGAIPPFSFHPKLKLVADPLLFERFDEIAFNAGMLDKSVILKTADYLRIAQPEL
                     VNFRRTA"
     gene            complement(3251..3355)
                     /locus_tag="NBI74_003164"
     CDS             complement(3251..3355)
                     /locus_tag="NBI74_003164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416302.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1933451.1"
                     /translation="MNDQMFVETLIITSSFFAIAAVLVLSVLLIERTG"
     gene            3525..3971
                     /locus_tag="NBI74_003165"
     CDS             3525..3971
                     /locus_tag="NBI74_003165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HCD1933452.1"
                     /translation="MFDVTLLILLGLAALGFISHNTTVAVSILVLIIVRVTPLSTFFP
                     WIEKQGLSIGIIILTIGVMAPIASGTLPPSTLIHSFLNWKSLVAIAVGVIVSWLGGRG
                     VTLMGSQPQLVAGLLVGTVLGVALFRGVPVGPLIAAGLVSLIVGKQ"
     gene            complement(3928..4749)
                     /gene="nimR"
                     /locus_tag="NBI74_003166"
     CDS             complement(3928..4749)
                     /gene="nimR"
                     /locus_tag="NBI74_003166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NimR"
                     /protein_id="HCD1933453.1"
                     /translation="MMHRLNLNGYEPDRHHEAAVAFCIHAGTDELTSPVHQHRKGQLI
                     LALHGAITCTVENALWMVPPQYAVWIPGGVKHSNQVTANAELCFLFIEPSAVTMPTTC
                     CTLKISPLCRELILTLANRTTTQRAEPMTRRLIQVLFDELPQQPQQQLHLPVSSHPKI
                     RTMVEMMAKEPVEWGSLRQWAGFFAMSERNLARLIVKETGLSFRQWRQQLQLIMALQG
                     LVKGDTVQKVAHTLGYDSTTAFITMFKKGLGQTPGRYIAGLTTVSPQSAKPDPRQ"
     gene            4846..6027
                     /gene="nimT"
                     /locus_tag="NBI74_003167"
     CDS             4846..6027
                     /gene="nimT"
                     /locus_tag="NBI74_003167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-nitroimidazole transporter"
                     /protein_id="HCD1933454.1"
                     /translation="MTCSTSLSGKNRIFLIAGILMIATTLRVTFTGAAPLLDTIRSAY
                     SLTTAQTGLLTTLPLLAFALISPLAAPVARRFGMERSLFAALLLICAGIAIRSLPSPY
                     LLFGGTAVIGGGIALGNVLLPGLIKRDFPHSVARLTGAYSLTMGAAAALGSAMVVPLA
                     LNGFGWQGALLMLMCFPLLALFLWLPQWRSQQHANLSTSRALHTRGIWRSPLAWQVTL
                     FLGINSLVYYVIIGWLPAILISQGYSEAQAGSLHGLLQLATAAPGLLIPLFLHHVKDQ
                     RGIAAFVALMCAVGAVGLCFMPAHAITWTLLFGFGSGATMILGLTFIGLRASSAHQAA
                     ALSGMAQSVGYLLAACGPPLMGKIHDANGDWSVPLMGVAILSLLMAIFGLCAGRDKEI
                     R"
     gene            6082..6429
                     /locus_tag="NBI74_003168"
     CDS             6082..6429
                     /locus_tag="NBI74_003168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HCD1933455.1"
                     /translation="MNIQCKRVYDPAEQSDGYRVLVDRLWPRGIKKTDLALDEWNKEI
                     TPSTELRKAFHGEVVDFATFREQYLAELAQHEQEGKRLADIAKKQPLTLLYSAKNTTQ
                     NHALVLADWLRSL"
     gene            complement(6451..6705)
                     /gene="yoaF"
                     /locus_tag="NBI74_003169"
     CDS             complement(6451..6705)
                     /gene="yoaF"
                     /locus_tag="NBI74_003169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing lipoprotein YoaF"
                     /protein_id="HCD1933456.1"
                     /translation="MKIISFVLPCLLVLAGCSTPSQPEAPKPPQIGMANPASVYCQQK
                     GGTLIPVQTAQGVSNNCKLPGGETIDEWALWRRDHPAGEK"
     gene            6888..7913
                     /gene="dgcP"
                     /locus_tag="NBI74_003170"
     CDS             6888..7913
                     /gene="dgcP"
                     /locus_tag="NBI74_003170"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416308.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcP"
                     /protein_id="HCD1933457.1"
                     /translation="MSDQIIARVSQSLAKEQSLESLVRQLLEMLEMVTDMESTYLTKV
                     DVEARLQHIMFARNSQKMHIPENFTVSWDYSLCKRAIDENCFFSDEVPDRWGDCIAAR
                     NLGITTFLSTPIHLPDGSFYGTLCAASSEKRQWSERAEQVLQLFAGLIAQYIQKEALV
                     EQLREANAALIAQSYTDSLTGLPNRRAIFENLTTLFSLARHLNHKIMIAFIDLDNFKL
                     INDRFGHNSGDLFLIQVGERLNTLQQNGEVIGRLGGDEFLVVSLNNENADISSLRERI
                     QQQIRGEYHLGDVDLYYPGASLGIVEVDPKTTDADSALHAADIAMYQEKKHKQKTPFV
                     THSALHS"
     gene            7946..8044
                     /locus_tag="NBI74_003171"
     CDS             7946..8044
                     /locus_tag="NBI74_003171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family small membrane protein"
                     /protein_id="HCD1933458.1"
                     /translation="MRIGIIFPVVIFITAVVFLAWFFIGGYAAPGA"
     gene            8047..8121
                     /gene="yoaJ"
                     /locus_tag="NBI74_003172"
     CDS             8047..8121
                     /gene="yoaJ"
                     /locus_tag="NBI74_003172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YoaJ"
                     /protein_id="HCD1933459.1"
                     /translation="MKKTTIIMMGVAIIVVLGTELGWW"
     gene            complement(8180..8428)
                     /locus_tag="NBI74_003173"
     CDS             complement(8180..8428)
                     /locus_tag="NBI74_003173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HCD1933460.1"
                     /translation="MGILSWIIFGLIAGILAKWIMPGKDGGGFFMTILLGIVGAVVGG
                     WISTLFGFGKVDGFNFGSFVVAVIGAIVVLFIYRKIKS"
     gene            complement(8576..8758)
                     /locus_tag="NBI74_003174"
     CDS             complement(8576..8758)
                     /locus_tag="NBI74_003174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1869 domain-containing protein"
                     /protein_id="HCD1933461.1"
                     /translation="MGKATYTVTVTNNSNGVSVDYETETPMTLLVPEVAAEVIKDLVN
                     TVRSYDTENEHDVCGW"
     gene            complement(8762..9121)
                     /gene="yeaR"
                     /locus_tag="NBI74_003175"
     CDS             complement(8762..9121)
                     /gene="yeaR"
                     /locus_tag="NBI74_003175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1971 domain-containing protein"
                     /protein_id="HCD1933462.1"
                     /translation="MLQIPQNYIHTRSTPFWNKQTAPAGIFERHFDKGTRPGVYPRLS
                     VMHGAVKYLGYADEHSAEPDQVILIEAGQFAVFPPEKWHNIEAMTDDTYFNIDFFVAP
                     EVLMEGAQQRKVIHNGK"
     gene            complement(9294..9932)
                     /gene="leuE"
                     /locus_tag="NBI74_003176"
     CDS             complement(9294..9932)
                     /gene="leuE"
                     /locus_tag="NBI74_003176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine efflux protein LeuE"
                     /protein_id="HCD1933463.1"
                     /translation="MFAEYGVLNYWTYLVGAIFIVLVPGPNTLFVLKNSVSSGMKGGY
                     LAACGVFIGDAVLMFLAWAGVATLIKTTPILFNIVRYLGAFYLLYLGSKILYATLKGK
                     NSEAKSDEPQYGAIFKRALILSLTNPKAILFYVSFFVQFIDVNAPHTGISFFILATTL
                     ELVSFCYLSFLIISGAFVTQYIRTKKKLAKVGNSLIGLMFVGFAARLATLQS"
     gene            complement(10059..10391)
                     /locus_tag="NBI74_003177"
                     /pseudo
     CDS             complement(10059..10391)
                     /locus_tag="NBI74_003177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001304302.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            complement(10475..11590)
                     /gene="rnd"
                     /locus_tag="NBI74_003178"
     CDS             complement(10475..11590)
                     /gene="rnd"
                     /locus_tag="NBI74_003178"
                     /EC_number="3.1.13.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HCD1933464.1"
                     /translation="MITTDDALASLCEAVRAFPAIALDTEFVRTRTYYPQLGLIQLFD
                     GEHLALIDPLGITDWSPLKAILRDPSITKFLHAGSEDLEVFLNVFGELPQPLIDTQIL
                     AAFCGRPMSWGFASMVEEYSGVTLDKSESRTDWLARPLTERQCEYAAADVWYLLPITA
                     KLMVETEASGWLPAALDECRLMQMRRQEVVAPEDAWRDITNAWQLRTRQLACLQLLAD
                     WRLRKARERDLAVNFVVREEHLWSVARYMPGSLGELDSLGLSGSEIRFHGKTLLALVE
                     KAQALPEEALPQPMLNLMDMPGYRKAFKAIKSLIIDVSETHKISAELLASRRQINQLL
                     NWYWKLKPQNNLPELISGWRGELMAEALHNLLQEYPQ"
     gene            complement(11672..13357)
                     /gene="fadD"
                     /locus_tag="NBI74_003179"
     CDS             complement(11672..13357)
                     /gene="fadD"
                     /locus_tag="NBI74_003179"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase FadD"
                     /protein_id="HCD1933465.1"
                     /translation="MKKVWLNRYPADVPTEINPDRYQSLVDMFEQSVARYADQPAFVN
                     MGEVMTFRKLEERSRAFAAYLQQGLGLKKGDRVALMMPNLLQYPVALFGILRAGMIVV
                     NVNPLYTPRELEHQLNDSGASAIVIVSNFAHTLEKVVDKTAVQHVILTRMGDQLSTAK
                     GTVVNFVVKYIKRLVPKYHLPDAISFRSALHNGYRMQYVKPELVPEDLAFLQYTGGTT
                     GVAKGAMLTHRNMLANLEQVNATYGPLLHPGKELVVTALPLYHIFALTINCLLFIELG
                     GQNLLITNPRDIPGLVKELAKYPFTAITGVNTLFNALLNNKEFQQLDFSSLHLSAGGG
                     MPVQQVVAERWVKLTGQYLLEGYGLTECAPLVSVNPYDIDYHSGSIGLPVPSTEAKLV
                     DDDDNEVPPGQPGELCVKGPQVMLGYWQRPDATDEIIKNGWLHTGDIAVMDEEGFLRI
                     VDRKKDMILVSGFNVYPNEIEDVVMQHPGVQEVAAVGVPSGSSGEAVKIFVVKKDPSL
                     TEESLVTFCRRQLTGYKVPKLVEFRDELPKSNVGKILRRELRDEARGKVDNKA"
     gene            complement(13562..14143)
                     /gene="yeaY"
                     /locus_tag="NBI74_003180"
     CDS             complement(13562..14143)
                     /gene="yeaY"
                     /locus_tag="NBI74_003180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Slp family lipoprotein YeaY"
                     /protein_id="HCD1933466.1"
                     /translation="MAVQKNVIKGILAGTFALMLSGCVTVPDAIKGSSPTPQQDLVRV
                     MSAPQLYVGQEARFGGKVVAVQNQQGKTRLEIATVPLDSGARPTLGEPSRGRIYADVN
                     GFLDPVDFRGQLVTVVGPITGAVDGKIGNTPYKFMVMQVTGYKRWHLTQQVIMPPQPI
                     DPWFYGGRGWPYGYGGWGWYNPGPARVQTVVTE"
     gene            complement(14183..14878)
                     /gene="tsaB"
                     /locus_tag="NBI74_003181"
     CDS             complement(14183..14878)
                     /gene="tsaB"
                     /locus_tag="NBI74_003181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HCD1933467.1"
                     /translation="MRILAIDTATEACSVALWNDGTVNAHFELCPREHTQRILPMVQD
                     ILTTSGTSLTDINTLAYGRGPGSFTGVRIGIGIAQGLALGAELPMIGVSTLMTMAQGA
                     WRKNGATRVLAAIDARMGEVYWAEYQRDENGIWHGEETEAVLKPELVHERMQQLSGEW
                     VTVGTGWQAWPDLGKESGLVLHDGEVLLPAAEDMLPIACQMFAEGKTVAVEHAEPVYL
                     RNNVAWKKLPGKE"
     gene            complement(14936..16846)
                     /locus_tag="NBI74_003182"
     CDS             complement(14936..16846)
                     /locus_tag="NBI74_003182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HCD1933468.1"
                     /translation="MTDDFAQDGQLAKAIPGFKPREPQRQMAVAVTQAIEKGQPLVVE
                     AGTGTGKTYAYLAPALRAKKKVIISTGSKALQDQLYSRDLPTVSKALKYTGNVALLKG
                     RSNYLCLERLEQQALAGGDLPVQILSDVILLRSWSNQTVDGDISTCVSVAEDSQAWPL
                     VTSTNDNCLGSDCPMYKDCFVVKARKKAMDADVVVVNHHLFLADMVVKESGFGELIPE
                     ADVMIFDEAHQLPDIASQYFGQSLSSRQLLDLAKDITIAYRTELKDTQQLQKCADRLA
                     QSAQDFRLQLGEPGYRGNLRELLANPQIQRAFLLLDDTLELCYDVAKLSLGRSALLDA
                     AFERATLYRTRLKRLKEINQPGYSYWYECTSRHFTLALTPLSVADKFKELMAQKPGSW
                     IFTSATLSVNDDLHHFTSRLGIEQAESLLLPSPFDYSRQALLCVPRNLPQTNQPGSAR
                     QLAAMLRPIIEANNGRCFMLCTSHAMMRDLAEQFRATMTLPVLLQGETSKGQLLQQFV
                     SAGNALLVATSSFWEGVDVRGDTLSLVIIDKLPFTSPDDPLLKARMEDCRLRGGDPFD
                     EVQLPDAVITLKQGVGRLIRDADDRGVLVICDNRLVMRPYGATFLASLPPAPRTRDIA
                     RAVRFLAIPSSR"
     gene            16978..17322
                     /locus_tag="NBI74_003183"
     CDS             16978..17322
                     /locus_tag="NBI74_003183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HCD1933469.1"
                     /translation="MTIVRIDAEARWSDVVIHNNTLYYTGVPENLDADAFEQTANTLA
                     QIDAVLEKQGSNKSSILDATIFLADKNDFAAMNKAWDAWVVAGHAPVRCTVQAGLMNP
                     KYKVEIKIVAAV"
BASE COUNT         4299 a         4418 c         4187 g         4476 t
ORIGIN      
        1 cagtacactg atccagacaa agtcgaatga tgtttctatt ttctatattt tccgccaact
       61 cagtttcatt tgtttaacct cgctggcgct cttttgttat gggaaagaca acatccttga
      121 caacaataag aaaaaaactg gcattctgtt gctggcgctg attccttttt tagtttttcc
      181 ccttctggca cacaatctga gcagttataa cgctgactat tctttgtatg tcgtcgatta
      241 ctgcccggac aaccatactg cgacctgggg aatcaactat acaaaaatat tggtttgttt
      301 gtgggcattt ttactgttct ttattatcat gcgtacacga ttagccagcg aactatggcc
      361 gttaatagca ttattatgtc tggcatcgct atgctgcaac ttacttctac tgactctgga
      421 tgagtataat tacaccatct ggtatatcag tcgcgggatt gaagtttcca gtaaactgtt
      481 tgttgtgtct tttctgattt ataacatttt tcaggaactg caactctcca gcaaactggc
      541 agttcataat gtgctgacca atatttataa tcggcgctac tttttcaaca gcgtagagtc
      601 attattgtcg cgacctgttg ttaaggactt ttgtgtcatg ctggttgata ttaatcagtt
      661 caaacgcatc aatgcccaat ggggacatcg tgtgggtgat aaagtgctgg tatcaattgt
      721 cgatattatc cagcaaagca tccgccccga tgatatttta gcgcgactgg agggtgaggt
      781 gtttggcttg ctatttaccg aactcaatag tgcccaggca aaaatcattg cggaacgtat
      841 gcgtaaaaat gtcgaactcc tgaccggctt tagtaacaga tatgatgttc ctgaacaaat
      901 gaccatcagt attggcacgg ttttttcaac gggtgacacg cataatatct cgcttgtcat
      961 gacggaagca gataaagcct tacgcgaagc gaaaagcgag gggggcaaca aagtgattat
     1021 tcatcatatt taagtgcaaa aatattcaca gccatgcttt ttgcgtggct tttgcataca
     1081 atttattact attaccccta aatttcccct ccccagcggc gcagagatga gtataattag
     1141 cgcccctgtg ccaggccgca atcgaacttt atctggtttt ctcgtttcac taaccgaagg
     1201 agtgccattt atcatgaaat tgcaccatag aatgctccgg cattttatcg ccgcaagtgt
     1261 cattgtgctg acatcttcct tccttatttt tgaacttgtc gccagcgaca gagcaatgag
     1321 tgcctatctg cgctatatcg tgcagagagc agactcttcc tttctttatg ataagtatca
     1381 gaatcagagt attgccgcgc atgtgatgcg cgctctcgct gctgagcagt cggaagtacc
     1441 gccagaacaa cggcgcgcta tctgcgaggc ttttgagtct gccaataata cccatggctt
     1501 aaacctgact gctcataaat accagggctt acgcggcaca ctacaaaccg catccactga
     1561 ctgcgacaca attgtggaag ctgcagcact attacccgct tttgatcagg cagtggaagg
     1621 caaccgccac caggatgatt acggttcagg tcttgggatg gccgaagaga aatttcacta
     1681 ttatctcgat ctcaatgacc gctatgtcta tttttatgag ccggttaatg ttgaatactt
     1741 tgcgatgaat aactggtcct tcctgcaatc aggaagtatt ggtatcgatc gcaaagatat
     1801 tgaaaaagta tttaccgggc gtaccgtatt atcgagcatt taccaggatc agcgtactaa
     1861 acagaacgtg atgagtttgc tgacgccggt atatgtcgca gggcagctaa aagggattgt
     1921 gctgctggat attaacaaaa acaatctgcg gaatatcttt tacactcatg accgccctct
     1981 cctctggcgt tttctcaatg tcacgctaac cgatacagat tcggggcgcg acattatcat
     2041 caaccagagc gaagataatc tgttccagta tgtcagttac gtccatgact taccgggcgg
     2101 cattcgtgtc tcgttatcca ttgatattct ttactttatc acgtcttcgt ggaaaagcgt
     2161 tctgttctgg attttgacgg cgttaatttt gctgaatatg gtgcggatgc acttccgctt
     2221 ataccaaaat gtgtcgcgag aaaatattag tgatgcgatg actggactct ataatcgcaa
     2281 aattttaacc cctgaactgg agcagcggtt gcagaaactg gtgcaatccg gttcttcggt
     2341 gatgtttatt gctattgaca tggacaagtt aaagcaaata aatgacaccc tcggtcatca
     2401 ggagggggat ttagcgatta cgttattagc tcaggcgatt aaacagtcga ttcgtaaaag
     2461 tgattatgcc atccgactcg gtggcgatga attctgcatc attcttgtcg attcgacgcc
     2521 gcaaattgca gcacaactgc ctgaacgtat cgaaaaacgt ctgcaacata tcgcgccgca
     2581 gaaagagatc ggcttctctt ccggtattta cgcgatgaaa gaaaacgata cgttacatga
     2641 tgcgtataaa gcttccgatg agcgtttata tgtcaataag cagaacaaaa acagccgttc
     2701 atgataacct tctgtggttg tttgcttgta atctcaggag catgaaatga ctgaaatggc
     2761 taaaggaagc gtgacgcatc agcggttaat cgctttatta tcacaagaag gtgctaactt
     2821 ccgcgtcgtt acccatgaag cggtaggtaa atgtgaagca gtgtcagaaa ttcgtggcac
     2881 cgctttaggc cagggggcta aggcactggt atgtaaagtc aaaggcaatg gcgtaaatca
     2941 acatgttctg gcgatcctcg ctgccgatca acaggccgat ctgagccaac tcgccagcca
     3001 tataggcgga ttacgcgctt cgttggccag cccggcggaa gtcgatgaac tcacaggctg
     3061 tgtcttcggc gcgatccccc ctttcagctt ccatccaaaa ctcaaactgg ttgccgaccc
     3121 cctactcttc gaacgatttg atgaaatcgc cttcaatgca ggcatgctgg ataaatccgt
     3181 tattttgaaa accgcggatt atttgcgtat tgcgcaacca gaactggtca atttccgccg
     3241 cactgcgtaa ctagcctgtc cgttcaataa gcagaacgga caaaaccagc acagcagcaa
     3301 tggcaaaaaa cgatgacgtg ataatcagtg tctcgacgaa catttgatcg ttcatagtat
     3361 gcccccggga gatagctgtt ttcacgttat tgttaacgta cacaaatggc agtttgatga
     3421 cagttcgtct gattttttat gcaaaaaacg taaagatttt ctacttcctg cctgcagcaa
     3481 gcgtaaagta agcaggctta ttattttttg gcaaggaaac cacgatgttt gatgtcactt
     3541 tgctgatcct gctcgggtta gctgcgctgg gctttatcag tcataacacc actgtcgccg
     3601 tttcaattct ggtgttgatt attgtccgcg tcacaccgtt aagcaccttt ttcccgtgga
     3661 ttgaaaaaca ggggctaagt atcgggataa tcattctgac gattggcgtt atggccccca
     3721 tcgccagcgg gacgctaccg ccctcaaccc tgattcactc gtttctgaac tggaaatcgc
     3781 tggtggcaat tgcagtcggc gttattgtct cctggctggg ggggcgcggc gtgacgttaa
     3841 tgggcagcca gccgcaactg gtcgccgggc tgctggtggg cactgtttta ggcgtggcgc
     3901 tgtttcgcgg cgtaccggtt ggcccgctta ttgccgcggg tctggtttcg ctgattgtgg
     3961 ggaaacagta gttaatcccg caatatatcg gcctggcgtt tgccccaggc ctttcttaaa
     4021 catagtaata aaggccgttg ttgaatcata ccccagtgta tgagcaactt tctgtacagt
     4081 atcgcctttc actaatccct gaagcgccat aatcagctgt aactgctgac gccactggcg
     4141 aaaactcaac cccgtctctt tcactatcag ccgggcaaga ttgcgttcac tcatcgcaaa
     4201 aaagccagcc cattgcctca atgagcccca ctcgacgggc tctttcgcca tcatctccac
     4261 catcgtgcgg attttaggat gagaagaaac gggcaagtgc agctgttgtt gtggttgctg
     4321 agggagttca tcaaacagga cctgaattaa tctgcgggtc atcggctcag cacgctgagt
     4381 cgtcgtcctg tttgccaggg ttaaaattaa ttcccggcat aagggcgata ttttcaatgt
     4441 gcaacatgtt gtcggcattg tcactgcgct gggctcgata aacagaaaac agagttcggc
     4501 gttagctgtc acttgattac tatgtttcac cccgcccggt atccaaaccg catattgtgg
     4561 cggcaccatc cacaaagcat tttctaccgt acaggtaatt gcaccatgta gtgccagtat
     4621 caactgccct ttgcggtgct gatgtacagg actggttaac tcatccgttc cggcgtgaat
     4681 acaaaatgcg accgcggctt catggtgtcg gtcgggttca tagccattga gattcaacct
     4741 gtgcatcatt ttgtccgaat ttagcgatat tttgtcattt tagcttgatt caacataaca
     4801 ataaaaacgg taaggtacag cctcgtttgt aacaatgaga agcatatgac ctgttcaact
     4861 tcattaagcg gtaaaaacag gattttcctt atcgctggca ttctgatgat tgccacaaca
     4921 ttacgcgtca cctttaccgg cgcagcaccg ttactggata cgattcgttc cgcttactcg
     4981 ctaacgacgg cgcaaaccgg cttattgacc accctgccat tattagcctt tgcgctgatc
     5041 tcaccgctgg ccgccccggt ggcgcgacgt tttggtatgg aacgtagcct gtttgccgcg
     5101 ttacttttga tctgtgcagg tatcgcaatt cgctctctcc cttcgcctta cttattattt
     5161 ggcggtacag cggtcattgg cggtgggatt gcattaggca atgtcttact gccaggatta
     5221 attaaacgcg atttccctca ttccgtcgcc agacttaccg gcgcatattc cctgacaatg
     5281 ggagctgcgg cggcactggg atcggcgatg gtagtgccgc tggctttgaa cggttttggc
     5341 tggcaaggcg cgttgctcat gctgatgtgt tttcctctgc tggctctttt tttatggctg
     5401 ccacagtggc gaagtcaaca acatgcaaat ttgagtacct cacgcgcctt acatactcgg
     5461 ggtatctggc gttcgccgct tgcctggcag gtaacattgt ttcttgggat caactcactg
     5521 gtctattacg tgattattgg ctggcttccg gcgatcctca tcagtcaagg ctatagcgaa
     5581 gcacaggcgg gttcactgca tggtttgctg caactagcca cagcagctcc cggtttgctg
     5641 atcccacttt tcttacatca tgtgaaagat cagcgtggta ttgcagcgtt cgttgccttg
     5701 atgtgcgcag tgggcgcggt tgggctctgc tttatgccag cacacgcgat cacctggact
     5761 ctgcttttcg gttttggttc cggcgcaaca atgatactgg ggttgacgtt cattggtctt
     5821 cgggcaagtt ctgcgcatca ggcggcggca ctctcgggga tggcacaatc cgtcgggtat
     5881 ttgctggcag cctgtgggcc gccgctgatg ggtaaaatac acgatgctaa cggtgactgg
     5941 tccgtaccac tgatgggtgt tgcaatactt tcactactga tggcgatttt cggactttgc
     6001 gccgggagag acaaagaaat tcgctaatat ccggtgctat agtgacgtga caaatcatgc
     6061 gtgaaaggga gaacaaatac gatgaatatt cagtgcaaac gcgtttatga tccggctgaa
     6121 cagagcgatg gttatcgcgt actggtcgac cgcctctggc cacgcggtat caaaaaaacc
     6181 gatttagccc ttgatgagtg gaataaagaa atcacgccgt caacggagct gcgcaaagcc
     6241 tttcacggcg aagtcgtcga ttttgcaacc tttcgcgagc aatatcttgc agaactggcg
     6301 caacacgagc aagaaggaaa gcggctggca gacatcgcca aaaaacagcc gctgaccctg
     6361 ctctactcag caaaaaacac cacgcagaac catgcgctgg tgctggcaga ctggctacgt
     6421 agcttgtgat tttagtacag catccggcgg ttatttttca ccagccggat ggtcccgtcg
     6481 ccacaatgcc cattcatcaa tcgtttcacc gcccggtaat ttgcaattgt tgctgacccc
     6541 ttgcgctgtc tgcacaggaa tgagcgtccc gcccttctgc tggcaataga ccgacgccgg
     6601 atttgccata ccaatctgcg gcggtttagg tgcttctggc tgagaagggg ttgaacaacc
     6661 agccaggacc agcaagcaag gcagaacaaa actgataatt ttcatttatt gatctcacat
     6721 atttatccag gcttagagta tcgcggtatc gttttgtttt gcagcactat ttttattaca
     6781 ttcactcaaa acatattacg ccttgtttca tctttgttga tgatgtttta tcatgcctgc
     6841 aaagattaaa taatcagcat ttacccgccg tatcctggag ttgtttcgtg tcagatcaga
     6901 ttatcgcccg cgtctcgcaa tcccttgcca aagaacagtc actggaaagt ctggttcgac
     6961 agcttctgga gatgctggaa atggtcactg atatggagtc aacctacctg accaaagtgg
     7021 atgtcgaagc gcgcctgcag catataatgt ttgcccgtaa tagccagaaa atgcacatcc
     7081 cggagaattt taccgtctcg tgggattact cgttatgcaa acgcgccatt gatgaaaact
     7141 gttttttcag cgatgaagtc cccgaccgtt ggggcgactg tattgcggca cgcaatcttg
     7201 gcatcaccac atttctgagc acgccaattc acttaccgga tggatcattc tatggcacgc
     7261 tttgcgccgc cagcagtgag aagcgccagt ggagtgaacg cgcggaacag gtcttacagt
     7321 tattcgccgg actgattgca caatatattc aaaaagaggc actggttgaa cagctgcgcg
     7381 aagctaatgc tgcgctgatt gcgcaatcgt ataccgactc gttaaccggt ctaccgaatc
     7441 ggcgggcgat ttttgaaaat ctgacgacgc tgttttctct cgcccggcat cttaaccata
     7501 agataatgat cgcgtttatc gatctggata acttcaaatt aatcaatgat cgttttggtc
     7561 ataatagtgg cgatctgttt ctcattcagg ttggcgagcg ccttaatacg ctccagcaaa
     7621 atggcgaagt tattggtcgt ctcggcggtg atgagttttt ggttgtttca ctgaacaacg
     7681 agaatgcgga tatttcgtcg ctgcgagaac gtattcaaca acaaatacgc ggagaatatc
     7741 acttaggtga tgttgatttg tattatcctg gtgccagtct tggcatagta gaagtcgatc
     7801 ctaaaacgac cgatgcagac agtgccctgc atgctgccga tatcgcgatg tatcaggaga
     7861 aaaaacataa acagaaaaca ccttttgtca cgcattcagc gctacattcc tgaggcgtat
     7921 tcacatcctt ttgattggtg ataacatgcg aatcggtatt atttttccgg ttgtaatctt
     7981 tattacagcg gtcgtatttt tagcatggtt ttttattggc ggctatgctg ccccgggagc
     8041 ataaagatga aaaaaacaac gattattatg atgggtgtgg cgattattgt cgtactcggc
     8101 actgagctgg gatggtggta acgtcacatc taaaaaatag caaaggctgc ctgtgtgcag
     8161 cctttgtgca atttacgcgt taacttttaa tcttcctgta gataaatagc acaacaatcg
     8221 caccaataac ggcaaccacg aagctgccaa aattgaagcc atcgacttta ccaaagccaa
     8281 acagcgtgct gatccatccg ccgactacgg caccgactat ccccagtagg atagtcataa
     8341 agaatccacc accatcttta cctggcatga tccacttcgc cagaataccg gcaataagcc
     8401 caaaaataat ccatgacaga atgcccattg tttcctcact tatctgtttt gcattagcgg
     8461 gttagtcgct gataaaaagc atagcacaac atcgggaggg taagatttgt gacgagcatc
     8521 acggaggaat tttttgcgat ggcgcagaaa ttgcgccatc aacgatcagt gataattacc
     8581 aaccacaaac atcatgttcg ttttccgtgt cataagaacg cacggtattc accagatctt
     8641 ttatcacttc agccgccact tctggcacca gcaaagtcat cggcgtctct gtttcataat
     8701 cgacagaaac gccattgctg ttattggtga cggtcacggt atacgttgct ttgcccatga
     8761 ttcatttccc gttatgaatg actttccgtt gttgcgcacc ttccatcagg acttcaggag
     8821 caacgaagaa gtcaatgttg aaataagtat cgtcagtcat ggcttcaatg ttgtgccact
     8881 tttctggagg gaacaccgca aactgccccg cttcgataag gatcacctga tcaggctctg
     8941 cactgtgttc atcagcgtag ccgagatatt tgaccgcccc atgcataacg gaaaggcgtg
     9001 ggtaaacccc cgggcgcgtt cctttatcaa aatgacgttc gaatattccg gcaggcgcag
     9061 tttgtttatt ccagaaaggc gttgagcgcg tatgaatata attctgtggg atttgcagca
     9121 tccttttccc tccttcggtg aatgcgctga aaacggttta ttccagccgt ttcagggtac
     9181 gcctgataat ttgcatttta aataccattt attggttact ttttagcacc gtatcagaga
     9241 agaatcaggg aggattacag gtgggaagcc cgcggcaacg cgggctgaaa gcatcaggat
     9301 tgcagcgtcg ccagtcgggc ggcgaaaccc acgaacatca aaccaatcag tgagttgccc
     9361 actttagcca gtttcttttt ggtacgtatg tactgcgtga caaaagcccc agaaataatc
     9421 aggaagctca aatagcagaa actcaccagt tccagcgtcg tcgccagaat aaagaatgaa
     9481 attcccgtat gtggggcatt aacatcgata aactgtacga aaaacgacac atagaacaaa
     9541 atggctttcg gattagtcag gctcaaaatt aacgcgcgct taaaaatggc accgtattgg
     9601 ggctcatcgg atttggcctc gctattttta cccttcaggg tcgcgtagag aattttactc
     9661 cccagataga gcaaataaaa cgcaccaaga taacgtacga tgttgaataa tatcggggtg
     9721 gtcttaatta atgtcgccac tccagcccat gccagaaaca tcaataccgc atcgccaata
     9781 aatacaccac aggccgcaag ataaccgcct ttcataccgc tactgacgct atttttgagt
     9841 acaaacaggg tatttggccc tggcaccaac acaataaaaa tggccccaac cagataggtc
     9901 cagtaattca gaaccccgta ttcagcgaac acattaacct ctttaattat ctttcgatca
     9961 tgcgcgatta aaggtgaata tgctaaccaa tctgtagcgg cttagaaagg agaaaatcag
    10021 gttttaactc gatatcaacc cgataattga atcattaatc aggcatgctc cagtgaaaaa
    10081 ttcgggtagt gctcggcaaa atactggcgt aaaaattcta ccgttatccg cactttcgct
    10141 gacgtcgaca gctttgaaac ataaacggcc cagacattcg ctggctggta atattccggt
    10201 agcacttgta ctaaatgacc actggcggga aacagcctgg cgacatcgat ttttacgtgg
    10261 tccggaaaaa caccgaaggc gaatattcct cgccaggcgg tagagtgaat gaaggaacag
    10321 agaatgaagt cgtcattcag gaatcggtat ttactcgtcg tggtgtcgag cgcattttac
    10381 gttaagctca aataagttca tttgaaccgc ctgctgttgg atttgtagtt tgccgaagcc
    10441 ggactgagcg cgcccagtcc ggcttcggaa gattttactg tggatattcc tgcaataaat
    10501 tgtgtaatgc ttccgccatc agctcaccac gccagccgga aatcagctcc ggcaaattgt
    10561 tctgcggttt cagtttccag taccagttca gcagttggtt gatttgccga cgcgatgcca
    10621 gcaattcggc gctgatcttg tgcgtttcgc tgacgtcaat aatcagcgac ttaatcgctt
    10681 taaacgcttt acgataaccc ggcatgtcca tcaggttaag catcggctgc ggtaaggcct
    10741 cttccggcaa tgcctgcgct ttttccacca gcgccagcaa cgttttaccg tggaagcgga
    10801 tttcgctacc ggataagccc aggctgtcca gttcgcccag acttcccggc atataacgcg
    10861 ctactgacca caaatgctct tcacgcacga caaagttcac cgccagatcg cgctctcgcg
    10921 ccttgcgcaa tcgccagtcg gctaacagtt gcagacaggc cagttggcgt gtgcgtaact
    10981 gccaggcatt ggtgatatca cgccaggcgt cttccggcgc aacgacttcc tgacgacgca
    11041 tttgcatcag gcggcattca tccagcgccg caggtagcca gccggaggcc tccgtttcta
    11101 ccataagctt tgcggtgatc ggtaacagat accagacatc cgccgctgcg tattcacact
    11161 gacgttcggt cagcggtctg gccagccagt cggtgcgcga ttcactcttg tccagcgtaa
    11221 cgccggaata ctcttccacc atggaagcga aaccccatga catcgggcgt ccgcagaagg
    11281 cagcaaggat ttgcgtgtca atcaagggtt gtggtaattc gccaaagaca ttgaggaaca
    11341 cttccagatc ttcactgcct gcatggagaa attttgtgat ggacggatcg cgcaggatcg
    11401 ctttcagcgg tgaccagtcg gtgatcccga gtggatcgat tagcgccaga tgctcaccat
    11461 caaaaagttg aattaaaccc agctgcgggt aataagtgcg cgtacgaaca aattcagtat
    11521 ccagggctat cgccggaaag gcacggacgg cttcacacaa agaagccagc gcatcgtccg
    11581 tggtaatcat ttggtaattc aaattgtatt ctctttagtg ggcgtcaaaa aaaacgccgg
    11641 attaaccggc gtctgacgac tgacttaacg ctcaggcttt attgtccact ttgccgcgcg
    11701 cttcgtcacg taattctcgt cgcaaaattt ttccgacgtt agatttcggt aactcatcac
    11761 gaaactccac cagcttcggt actttgtagc ccgtgagctg acggcggcaa aaggtcacca
    11821 gtgactcttc ggtaagcgat ggatcttttt tcactacgaa gattttcacc gcttcaccac
    11881 tggagccgga aggtacgcca acagccgcga cttcctgtac gccaggatgc tgcatgacga
    11941 catcttcaat ctcgttggga tagacgttaa aaccggaaac cagaatcata tcttttttac
    12001 gatcgacaat gcgcaggaat ccttcttcat ccatcaccgc gatgtcgccg gtgtgtaacc
    12061 agccattttt gatgatttca tcggtagcat caggacgctg ccagtaaccc agcatcacct
    12121 gcggtccttt gacacaaagc tcacctggtt gaccaggtgg tacttcatta tcatcatcat
    12181 ccaccagttt ggcttccgtc gacggcaccg gcaaaccgat gctaccacta tgataatcaa
    12241 tatcatacgg gttaacgctg accagcggcg cgcactcggt caggccataa ccttccagca
    12301 gatactgtcc agtcagttta acccaacgtt ctgccaccac ttgctgaaca ggcatcccac
    12361 cgcctgcgga aagatgcaga ctggagaaat ccagctgctg gaactcttta ttgttcagca
    12421 acgcattgaa caaggtgtta acaccagtga tagcggtaaa cggatatttc gctaactctt
    12481 ttaccagccc tggaatatcg cgcgggttag tgataagcag gttctgcccc cccagttcga
    12541 taaacagcag gcagttaatg gtcagggcaa aaatgtgata cagcggcagc gccgtcacca
    12601 ccagctcttt gcccggatgc aagagcggac cataggtcgc gttaacctgt tccaggttcg
    12661 ccagcatatt gcggtgagtc agcatcgcgc ctttcgccac accagtggtg ccgccggtgt
    12721 attgcaggaa agctaaatct tccggcacca gttctggttt gacgtactgc atccggtagc
    12781 cgttgtgcag ggcgctacga aatgaaatgg catctggcag atggtatttc ggcaccagac
    12841 gcttgatgta tttaacaacg aaattgacta ccgtgccttt tgccgtagat agctgatcgc
    12901 ccatacgggt cagaataacg tgctgaacgg cggttttatc aaccactttt tccagagtgt
    12961 gggcaaagtt agacacgata acaatcgccg atgcgccgct atcgttaagc tgatgctcaa
    13021 gctcacgcgg ggtatacaac gggttaacgt ttacgacgat catcccggca cgcaaaatgc
    13081 caaacagcgc caccggatat tgcaataagt taggcatcat caacgcaacg cgatcgcctt
    13141 tcttcagccc caacccttgt tgcaaataag cggcaaacgc gcgactgcgt tcttccagtt
    13201 tgcggaaggt catcacctcc cccatattca caaacgcggg ttgatcggcg tagcgcgcga
    13261 ccgactgctc aaacatatct accagagatt gataacggtc agggttgatc tccgtcggaa
    13321 cgtccgcggg ataacggtta agccaaacct tcttcaattc ttcacctcta aaatgcgtgt
    13381 tcgtcgtcat cgcaacccca agtgatagac atgctgttaa cataatatta actcatctta
    13441 ccagcttgat aattacccaa ggaaaaggtt gcgaagcgcg tcactattta tttttatctt
    13501 taccgtaaga atgcagaaac agcggaccgg ccgctgtttc tttttcttta aaaacaagca
    13561 attattcagt tacaactgtt tgtactctcg cggggccggg attataccag ccccatccgc
    13621 cgtagccata gggccagcca cgaccgccat aaaaccatgg atcaatcggc tgaggcggca
    13681 taatcacctg ctgggttaaa tgccaacgtt tgtaacccgt tacttgcatc accataaatt
    13741 tatagggcgt attaccgatt ttgccgtcaa ccgcaccggt gattggcccg actaccgtca
    13801 ccagttgtcc gcggaaatcc accgggtcca gaaaaccgtt cacatcggca taaatgcgac
    13861 cgcgagaagg ttctcccagc gtcggtctgg ctccgctgtc cagtggtacg gtggcaattt
    13921 ccaggcgggt tttcccttgc tggttttgta ccgcaaccac tttgccgcca aagcgagcct
    13981 cctgaccaac gtacagctgc ggcgcactca tcacccgaac taaatcttgt tgcggcgtgg
    14041 gactactgcc tttaatggcg tccggcacag taacacaacc gctgagcatt agcgcaaacg
    14101 tacctgccag tatgcctttg ataacattct tttgaaccgc catgatgcga ctcctttttt
    14161 tctcagggca tactcttaag attcattctt tgcccggaag tttcttccat gcgacattgt
    14221 tacgtaaata aactggttcg gcatgttcca ccgccaccgt tttaccctcg gcaaacatct
    14281 ggcacgcaat cggcagcata tcttctgcag cgggcagtaa cacttcgcca tcgtgcaaaa
    14341 ccagcccgct ctctttaccg agatccggcc aggcttgcca gcccgtccct accgtcaccc
    14401 attcgccgga aagctgttgc attcgttcat gaacgagttc gggtttgagt acggcttcgg
    14461 tttcttcacc atgccagata ccgttttcat cacgctgata ttcggcccag taaacttcgc
    14521 ccattcgcgc gtcaatagct gccagcacgc gggttgcgcc gtttttgcgc cacgcccctt
    14581 gcgccatcgt cattagcgtg gagacgccaa tcatcggcag ctctgcgcca agtgccagcc
    14641 cttgcgcaat accaatacca atgcgcacgc cggtaaagct accggggccg cgcccgtaag
    14701 ccagagtgtt tatgtcagtc agggaagttc cgctggtggt caggatatcc tgcaccatcg
    14761 gtaagattcg ttgagtatgt tcacgagggc aaagctcaaa atgagcgttg acagtaccgt
    14821 cgttccacag ggcgacagag caggcctctg tcgcggtatc gatagccaga attcgcatgg
    14881 gtcttcgtgc ttagatcaat aaaaaggcgc gcatcatacc atactccgta acaaattacc
    14941 tggaggatgg tatcgcaagg aaacgaaccg cacgggcaat gtcacgggtg cgcggcgctg
    15001 gcggcagact ggcgagaaac gtcgcgccgt aagggcgcat caccagccga ttgtcgcaaa
    15061 tcaccagcac gccgcgatcg tcggcgtcgc gaatcaatcg ccctaccccc tgcttgagag
    15121 taatgaccgc atccggcagt tgtacttcat cgaacggatc accaccgcgc aaacggcaat
    15181 cttccatgcg tgcttttaat agtggatcat ccggcgaggt aaacggcaat ttgtcgataa
    15241 tcaccaatga caatgtatcg ccacgcacgt ccaccccttc ccagaaactg ctggttgcca
    15301 caagaagcgc attaccggcg ctgacaaatt gctgcaacag ttgccctttg ctggtttccc
    15361 cctgcaacaa aacgggaagc gtcatggtag cgcggaactg ctcggccaga tcgcgcatca
    15421 tggcgtgcga ggtacaaagc ataaaacaac gaccgttgtt agcttcgatg atcggtcgca
    15481 gcattgccgc cagttgccgt gcggaacccg gttgattggt ttgcggcagg ttgcgcggca
    15541 cacagagtaa cgcctggcgg ctgtaatcaa aggggctggg taacagcaat gactctgcct
    15601 gttcaatgcc aagccgcgag gtgaaatgat gcagatcgtc gttcaccgac agcgttgctg
    15661 aggtaaagat ccagctaccg ggtttttgcg ccattaactc tttgaattta tccgccacgc
    15721 tgagcggcgt gagagccaga gtaaaatggc gcgaagtgca ttcgtaccag tagctgtagc
    15781 ccggctgatt gatctctttt agccgtttca gccgtgtgcg atataacgtg gcgcgctcaa
    15841 atgccgcatc cagcaaggcg gaacgcccca gcgacagttt cgccacgtca taacaaagtt
    15901 ccagagtgtc atcgagcagt aaaaatgccc gctgaatttg cggattagct aacagctcac
    15961 gcaggttacc gcgataacca ggctcaccga gttgcaggcg aaaatcctgc gcactctggg
    16021 caagacggtc cgcgcacttt tgtaactgct gggtgtcttt taattcggtg cgataggcga
    16081 tggtgatgtc ttttgccagg tcgagcagtt gtcgactgga gagtgactga ccaaaatact
    16141 ggctggcaat gtcgggcagc tggtgggctt cgtcgaagat catgacgtcc gcttccggga
    16201 tcagttcgcc aaaaccgctc tctttcacca ccatatccgc cagaaacaga tgatgattca
    16261 ccaccaccac atcggcgtcc atcgcttttt tgcgtgcttt gaccacaaag caatctttat
    16321 acatcgggca gtcgctgcca agacagttgt cgttggtgct ggtgaccagc ggccacgcct
    16381 gtgaatcttc cgccacgctg acgcaggtgc tgatatcacc atcgactgtt tgattagacc
    16441 aggagcgcag caggatcaca tcgcttaaga tttgtaccgg cagatcgccc cccgccaacg
    16501 cctgctgttc gagacgttcg aggcagaggt agtttgagcg ccctttcagc aacgccacgt
    16561 tgcccgtgta tttcaacgcc tttgagaccg ttggcaaatc gcggctgtag agctgatcct
    16621 gcaacgcttt tgagccggtc gagattatga ctttcttttt cgcccgcagc gcaggggcca
    16681 ggtaagcgta ggttttgccc gtaccggttc ctgcttccac caccagcggc tggccttttt
    16741 ctatcgcctg ggtgacggct accgccatct gtcgctgtgg ttctcgcggc ttaaagcctg
    16801 gtatcgcttt cgccagctga ccgtcttgtg caaaatcgtc cgtcacacta cccccggttg
    16861 atttgaacag ggattatgtc aggatgaggg cgctttcgcc agttgaagtg gtgacggcga
    16921 cctcacattg tggcagtctt tgcagcacga aaatggaagt ataatgagga caaaatgatg
    16981 actatcgttc gtatcgatgc cgaagcccgc tggtctgatg tggtaatcca caacaacacg
    17041 ctctactaca ctggtgtacc ggaaaacctc gacgccgatg cctttgagca aaccgccaac
    17101 acgctggcgc agattgacgc cgtgctggaa aaacagggca gcaataaatc gagcattctg
    17161 gatgccacca ttttcctggc cgataaaaac gacttcgcgg cgatgaataa agcgtgggat
    17221 gcctgggttg tcgcgggtca tgcgccggtg cgctgcacgg tacaagcagg tttgatgaac
    17281 ccgaagtata aagttgaaat taagattgtg gctgcggtgt aagctttatc gaagcaaaat
    17341 aagtcagacg ataatttatc gacaatactg gtcggttttg
//
LOCUS       DAHHTG010000093        17378 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.093, whole genome shotgun sequence.
ACCESSION   DAHHTG010000093 DAHHTG010000000
VERSION     DAHHTG010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17378)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17378)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17378
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.093"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            119..637
                     /locus_tag="NBI74_003184"
     CDS             119..637
                     /locus_tag="NBI74_003184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HCD1933470.1"
                     /translation="MMKFKKCLLPVAMLASFTLAGCQSNADDHAADVYQTDQLNTKQE
                     TKTVNIISILPAKVAVDNAQNKRNAQAFGALIGAVAGGVIGHNVGSGSNSGTTAGAVG
                     GGAVGAAAGSMVNDKTLVEGVSLTYKEGTKVYTSTQVGKECQFTTGLAVVITTTYNET
                     RIQPNTKCPEKS"
     gene            653..1192
                     /locus_tag="NBI74_003185"
     CDS             653..1192
                     /locus_tag="NBI74_003185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5384 family protein"
                     /protein_id="HCD1933471.1"
                     /translation="MKKVFLCAILASLSYPAIASSLQDQLSAVAEAEQQGKNEEQRQH
                     DEWVAERNREIQQEKQRRANAQAAANKRAATAAANKKARQDKLDAEATADKKRDQSYE
                     DELRSLEIQKQKLALAKEEARVKRENEFIDQELKHKAAQTDVVQSEADANRNMTEGGR
                     DLMKSVGKAEENKSDSWFN"
     gene            complement(1285..2862)
                     /gene="guaA"
                     /locus_tag="NBI74_003186"
     CDS             complement(1285..2862)
                     /gene="guaA"
                     /locus_tag="NBI74_003186"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HCD1933472.1"
                     /translation="MTENIHKHRILILDFGSQYTQLVARRVRELGVYCELWAWDVTEA
                     QIRDFNPSGIILSGGPESTTEENSPRAPQYVFEAGVPVFGVCYGMQTMAMQLGGHVEA
                     SNEREFGYAQVEVVNDSALVRGIEDALTADGKPLLDVWMSHGDKVTAIPSDFVTVAST
                     ESCPFAIMANEEKRFYGVQFHPEVTHTRQGMRMLERFVRDICQCEALWTPAKIIDDAV
                     ARIREQVGDDKVILGLSGGVDSSVTAMLLHRAIGKNLTCVFVDNGLLRLNEAEQVLDM
                     FGDHFGLNIVHVPAEDRFLSALAGENDPEAKRKIIGRVFVEVFDEEALKLEDVKWLAQ
                     GTIYPDVIESAASATGKAHVIKSHHNVGGLPKEMKMGLVEPLKELFKDEVRKIGLELG
                     LPYDMLYRHPFPGPGLGVRVLGEVKKEYCDLLRRADAIFIEELRKADLYDKVSQAFTV
                     FLPVRSVGVMGDGRKYDWVVSLRAVETIDFMTAHWAHLPYDFLGRVSNRIINEVNGIS
                     RVVYDISGKPPATIEWE"
     gene            complement(2931..4397)
                     /gene="guaB"
                     /locus_tag="NBI74_003187"
     CDS             complement(2931..4397)
                     /gene="guaB"
                     /locus_tag="NBI74_003187"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004151980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HCD1933473.1"
                     /translation="MLRIAKEALTFDDVLLVPAHSTVLPNTADLSTQLTKTIRLNIPM
                     LSAAMDTVTEARLAIALAQEGGIGFIHKNMSIERQAEEVRRVKKHESGVVTDPQTVLP
                     TTTLREVKELTERNGFAGYPVVTEENELVGIITGRDVRFVTDLSQPVSVYMTPKERLV
                     TVREGEAREVVLAKMHEKRVEKALVVDDEFHLIGMITVKDFQKAERKPNACKDEQGRL
                     RVGAAVGAGAGNEERVDALVAAGVDVLLIDSSHGHSEGVLQRIRETRAKYPDLQIIGG
                     NVATAAGARALAEAGCSAVKVGIGPGSICTTRIVTGVGVPQITAVADAVEALEGTGIP
                     VIADGGIRFSGDIAKAIAAGASAVMVGSMLAGTEESPGEIELYQGRSYKSYRGMGSLG
                     AMSKGSSDRYFQSDNAADKLVPEGIEGRVAYKGRLKEIIHQQMGGLRSCMGLTGCGTI
                     DELRTKAEFVRISGAGIQESHVHDVTITKESPNYRLGS"
     gene            4559..5926
                     /gene="xseA"
                     /locus_tag="NBI74_003188"
     CDS             4559..5926
                     /gene="xseA"
                     /locus_tag="NBI74_003188"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000937953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HCD1933474.1"
                     /translation="MLPSQSPAIFTVSRLNQTVRLLLEHEMGQVWISGEISNFTQPAS
                     GHWYFTLKDDTAQVRCAMFRNSNRRVTFHPQHGQQVLVRANITLYEPRGDYQIIVESM
                     QPAGEGLLQQKYEQLKAKLQAEGLFDLQYKKSLPSPAHCVGVITSKTGAALHDILHVL
                     KRRDPSLPVIIYPTAVQGDDAPGQIVRAIELANKRNECDVLIVGRGGGSLEDLWSFND
                     ERVARAIFASRIPIVSAVGHETDVTIADFVADLRAPTPSAAAEVVSRNQQELLRQVQS
                     ARQRLEMAMDYYLANRTRRFTQIHHRLQQQHPQLRLARQQTMLERLQKRMSFALENQL
                     KRAGQQQQRLTRQLVHQNPQSRIHRAQTRIQQLEYRLAETLRAQLSATRERFGNAVTH
                     LEAVSPLSTLARGYSVTSAADGAVLKQVKQVKVGDTLTTRLGDGVVVSEVSAVTKTRK
                     PRKKT"
     gene            complement(5980..14061)
                     /locus_tag="NBI74_003189"
     CDS             complement(5980..14061)
                     /locus_tag="NBI74_003189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001525370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RatA-like protein"
                     /protein_id="HCD1933475.1"
                     /translation="MSVCLKPGKIIVLLGMLAAFMLSDFARAEVEWQTYPGSTGEFNG
                     TVPIADSASVPVYQGSVQLDPAASHDVAFSAKPNEFSVDDDAANLIVANPQDSEGDQF
                     STPPALRWENQTPPTVSLVWADAATPDTPLNPQPIANRSFCAQGLAGRSLVAWPQIDA
                     QQTIPLLYLLTSTGYPYEGTVVLADQKVTLNIAPAQGDLISVSASGYNETLGAAKTTV
                     GGTITLTVTTKDCQGNVAGNIPFIIKRKDAQNRQGAVNNTAPVVLDSTELTTTVTEYR
                     GTSDANGTATITVTQPNGPGVKTPLVVGISGIAQTSEAAVIFTVLTSPDVAQATMWGH
                     MAETVEAHGYTFSRPKLAAEVSNENATVVDHNETWSTFTWSGADSHCTVLPGMRHFGA
                     LATVIPSTVQTVLGWPMQGDYYWSSLAGLTGQHHAADVSNRGETQKPDSTTFLVSCVD
                     KPAPDVEPKIVLTPENYDDTAQAMKAKVGEDATMRLAITDTKNNDQPLAYYYFSLHLD
                     DGVNRKNQTDTAWEAHPVQIAGGSNFRQVDAHTYEGMTDANGQASLTLSQPGGAGVKT
                     HITARMRSDFNATDAKDVIFTVITSPDSDKARMWGHMRGIIESGSLYKRPLLADETEH
                     ELGTVRENNEDWALYDQNTSMQAECGVGHIPRQSSLESLFSAHPGNAIGTEYGWPTAQ
                     QGYLSAVEQATHSSVDLGNGSVDSYSGFKPNYLSCSGNEMVANVEVSTDHDVSVGTQA
                     QAKVGDTIVMTVRTINSLNNIPVPFTAFTITKGMGYNRAGQVSGFDDPSSGAITMDNT
                     QYGTSQSSKVYAGITDARGVATVEIKQPQGVGLKTVLSVTPVNSYLPNTVNYSVIFTT
                     PTSPDVTGAQMWGHMDETITVDSSTFTRPKLASEVASPDGTLTENNEIWARVSQANTS
                     STSKGGCDTNMLPRRSQLSALYNANSGNAVQTAHGWPTQRQPYWSNSPADKTPHFFTI
                     ALNDGAQTIGGDTPVYVSCLKTANKPASSITLEVVDKAQWNTGNSAATLKKGETLQVK
                     VTVKDAQGNALADMPFTLNRGDGYTRSGEKHVAGSGDALVAPVVVNGGLADETTLNDT
                     ATVYTAMTDNDGSKILNITRPDTHGTKTALTAMLYSDATKKSSIDTIFTVVTSPDSRL
                     AKMWGHMPETVTAADGTVFKRPLLLKELAYQTGRTSTSEDNENWALFNINYASFSTTY
                     SGCGTNYIPTQAGLTSLFANNAGNTMKTVQGWPVATRYLSNTSDNGSMEQRNYKAVDL
                     SNGTSAAVSSTTLELLTCQTAPIAAVSQILLEAADPVTLDTTYNVVKAKKSEESVVRV
                     ITKDAQGNLVGNTAFILTRANSVSRANSSATMSVGALTVTDAWGNTRNNFQSTSETIY
                     GVTGADGSTTFTLKQDNSTGLRTDLTAKLDTSSSVKAMLPVVFTVVTSPDSPKANFWG
                     HMAETVTASDGSVYKRPLLFGELTNNYARYSTFENGESWARFTSTQSADTSMNGCGTD
                     YMPTLDGLKSLYDANRGNAMNTVQGWPVNMSYLTNTPSNTQTGSRYYNVVQLNSGAVS
                     QIVSTVLALQTCRTTPLMTASQITLEASDPGQFVSIDSTLSAVKAKKGDEVSIRISTK
                     DAQGNLVGNTPFALKHANSINRQNVTSSQKVSVTTAAGATVDTSATTILYGVTGPDGT
                     TTMTLRQDASTGLRTDFYALLNDTGVSSDTLSVIFTVTTSPDTPLATNWGHMAETFTS
                     SEGVTFKRPFLKAELSGGTAKTANNEVWSLLTATEKADVSKAGCDEAYQPLTTDMQGL
                     YVDYPNGQLATVLGLPTAAGNWWDYDMMAVAGGSWSNQAFVPGNGQLIQASSSYTAIV
                     MCLVEPHTEAVTIELTSTAQDAAKSLSNDGRPSAVAKKGELIPLTVTVRDSAGNPMPY
                     ADFTLSRETTLDRAKATVNTSADDLTVTALVPVSRDSVLTASGAKTLATTGSDGTATF
                     EVGQYSTTGLATPLTVTLARDTTKVATLDVIYTVITSPDSPSAKFWGHMPDTFTSSAG
                     VTFKRPLLKTETSSGLSISSNGEVWSYMSNLQNLTSTDCPLENQPRSKELLDLYSDHP
                     NGAIMTDLGLPMNAGNWWAYDMAILGTTTWSYQTVSLRTGAIAKNSATNALMLCLAQP
                     HPAGVSVTLTSSALDDARTLSNGGRPSASVKKDNPLPIVVMVKDRDGNPLAGEAITLR
                     RDNAMSRSGTIVNTAANVMVLTELAPVSATFTLASHGTQWLGFTGSDGTAIFNVEQPD
                     TVGLATPFTAILARDTETTTVLDAIFTVVTSPDSAMAAYWGHMPDTVTAENGVVFERP
                     KLWKELPSTSGVSKVNSNNEVWPVFNATQRADSNLSPCEAARRPLSDDLESLYARYPG
                     NTLTTQIGWSTQYTWWALDNWLGGSNPQVVGLNNGSAWGTVNTAYQGCLVNPRATVSS
                     VTLTSTALDSSTQAAKVKKGDAVPVVVTVKDSVGKPVPNISFILKRGDATPRNSGATL
                     YGDVDTMDDLTVQPSSGAAVTLADSGNTIDGVTGADGTASFTVGQDNTPGYKTPLTVT
                     LTDNATITATLDTIFTVPTSPNVATAYFWGHMADTATVSGKMLHRPLLKSELPSGVTA
                     AATPNVNNETWALAHVIDSTKWDVARQCGSMNNVPSSAELQTLHSGFSTLGWPSSISF
                     PYLSTDKAGSFYCGVEEGSGSLNCGIQPAKTPGFATCFQ"
     gene            complement(14171..15163)
                     /locus_tag="NBI74_003190"
     CDS             complement(14171..15163)
                     /locus_tag="NBI74_003190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000802324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1933476.1"
                     /translation="MKPHMKRGLTKVALALMLAGYCAVPVAMAEDAAWVASGTTAEFE
                     GTIPWLYREGGNATINSEDADHIKVTSDSKGIRPAGSESDKRLYSGDTITLGWDIGDT
                     EGDIDDGSAGIDAKTTATIKWYSYSDNAGGGKTELTAAAGKTSYKITDGDRGRYIGVE
                     IQPITQTGNPFQGTSLTLLDISTASGGGSDTDNVDPGPVVNQNLKVAIFEKDTSTNLI
                     GGNTAIALNKTYVAKLYSDENQNGKYDAGTDVDVTANYDFAWVFNGNSKQLAAAGGIA
                     NASFDNNDIVIPQTNEQARTSLNGSDRDGKTGLAIPANGDGVQGYTLSIIYKHH"
     gene            complement(15219..17372)
                     /gene="sinH"
                     /locus_tag="NBI74_003191"
     CDS             complement(15219..17372)
                     /gene="sinH"
                     /locus_tag="NBI74_003191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000946972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intimin-like inverse autotransporter SinH"
                     /protein_id="HCD1933477.1"
                     /translation="MMLTFISGAAFAAPEINVKQNESLPDLGSQAAQQDEQTNKGKSL
                     KERGADYVINSATQGFENLTPEALESQARSYLQSQITSTAQSYIEDTLSPYGKVRSNL
                     SIGQGGDLDGSSIDYFVPWYDNQTTVYFSQFSAQRKEDRTIGNIGLGVRHNFDKWLLG
                     GNIFYDYDFTRGHRRLGIGAEAWTDYLKFSGNYYHPLSDWKDSEDFDFYEERPASGWD
                     IRAEAWLPAYPQLGGKIVFEHYYGDEVALFGTDNLEKDPFAVTLGVKYQPVPLIAVGT
                     DFKAGTGDNTDTTVNATLNYQFGVPLKDQLDPDKVSAAHSLMGSRHDFVERNNFIVLE
                     YKEKDPLDVTLWLKADAMNEHPECVIKDTPEEAIGLEKCKWTINALVNHHYKIVAASW
                     QAKNNAARTLVMPVIKENTLTEGNNNHWNLVLPVWQYSSDKAEQGKLNTWRVRLALKD
                     EKGNRQNSGVVEITVQQDRKIELIVNNIANVPEENNHSHEASAQADGVDGVVMDLDIT
                     DSFGDNTDRNGNVLPQDNLNPQLFDANDKKVTLTNNPCTTETPCVFIAKQDKEKGTVT
                     LSSTLPGTFRWKAKAAPYDDSNYVDVTFLGADIGGLNAFIYRVGAAKSVNLIGNKEPL
                     PLNSRYRFVLWRDANKDGVFQLSEKLTDEEMKQYDYQWEFTGHSVNGNTGAQANTTNV
                     DIEIPATNKEAAAKFSAQVTDGVQGYGLQVNYSKT"
BASE COUNT         3940 a         4736 c         4398 g         4304 t
ORIGIN      
        1 attattcccc acatgaattt taataaattt aatctattcc tttatacgca atacatttac
       61 tttcctcttt tgatgatctt aaatgtctta tttttcgtaa tgtgtataac aaggaatagt
      121 gatgaaattt aaaaaatgtc ttctgcctgt ggcaatgtta gcgtcattca ctctggcagg
      181 atgccagtca aatgctgatg atcatgccgc cgatgtttat caaaccgatc aactgaatac
      241 caaacaagaa actaaaaccg ttaatattat ttccattctt cccgcaaaag ttgccgtaga
      301 caacgcccaa aataaacgga acgcacaagc cttcggcgcg cttattggcg ctgtcgctgg
      361 cggtgttatc ggccacaacg tcgggtctgg cagcaattcc ggaacgacgg caggtgcagt
      421 tggcggcgga gctgtaggcg cggcagcggg ttctatggtg aatgataaaa ccttagtgga
      481 aggtgtttct ttaacatata aggaaggcac caaagtgtat acctccaccc aagtgggtaa
      541 agagtgccag tttacgacag gtttagccgt tgttattacc acgacgtata acgaaacgcg
      601 tattcagcca aataccaaat gtcctgaaaa gagctaataa tcaggaggag tcatgaagaa
      661 agtttttctt tgcgccatct tagcctcctt aagctatccg gctatcgcct catcattgca
      721 ggatcaactc tcggctgtcg cagaagcgga acagcaaggt aaaaatgaag agcaaaggca
      781 gcatgacgaa tgggtcgcgg agcgcaacag ggaaatccag caagagaagc aacgtcgcgc
      841 aaacgcccag gccgcggcta ataaaagagc ggcaacggca gcagcgaata agaaagctcg
      901 tcaggataaa ctggacgccg aagccactgc ggacaaaaaa cgcgatcaaa gttatgaaga
      961 tgagctacgt agcttagaga ttcagaaaca aaaactggcg ctggcgaaag aagaagcccg
     1021 cgttaagcgc gaaaacgaat ttatcgatca ggaactgaag cacaaagctg cgcaaaccga
     1081 tgtggtgcaa tctgaagctg acgcaaacag aaatatgact gaaggcggtc gcgatctgat
     1141 gaaaagcgtg ggtaaagcag aagagaataa atcagatagc tggtttaact aaacgatgtt
     1201 agtaacttca aacctataat tctttaggat aaaaaaccct ctgtagtaac agagggtttt
     1261 gttcattcat agtgcagggt caaatcattc ccactcaata gttgctggtg gcttgccgct
     1321 gatgtcatac accacgcggg aaataccatt cacttcattg ataatgcggt tggaaactcg
     1381 accgaggaaa tcgtacggca gatgtgccca gtgtgcggtc ataaagtcga tggtttcgac
     1441 agcacgcaga gagacaaccc agtcatactt acgaccatcg cccattacgc caacggaacg
     1501 tactggcagg aagacggtga acgcctggct gactttgtcg tacaggtccg ctttacgcag
     1561 ttcttcaatg aagatggcgt cagcacggcg cagcaggtca caatactctt tcttcacttc
     1621 ccccagaaca cgaacgccaa ggcctggtcc cgggaacggg tgacggtaca acatgtcata
     1681 cggcaggccc agctccagac caatcttacg cacttcgtct ttgaacagct ctttcagcgg
     1741 ttcaaccagg cccatcttca tctctttcgg cagcccaccc acgttgtggt gagatttgat
     1801 gacgtgtgct ttaccggttg cagaagcagc agattcgata acgtcagggt agatggtgcc
     1861 ctgcgccagc cacttcacgt cttccagttt cagcgcttct tcatcgaata cttcaacgaa
     1921 aacgcgcccg atgattttac gttttgcttc cggatcgttt tcgccagcca gcgctgacaa
     1981 gaagcgatct tctgccggta cgtgaacaat gttcagacca aagtgatcgc caaacatatc
     2041 cagaacctgc tctgcttcgt tgagacgcag caggccgttg tcgacgaata cgcaagtcag
     2101 gtttttaccg atagcgcggt gcaacagcat tgcggttacg gaggaatcca cgccaccaga
     2161 gaggccgagg atgactttat cgtcgcctac ctgctcgcgg atgcgggcta cagcgtcgtc
     2221 gataattttc gctggcgtcc acagggcttc acactggcag atatcacgca caaaacgctc
     2281 cagcatgcgc ataccctggc gggtgtgagt cacttccggg tggaactgta cgccatagaa
     2341 gcgtttttct tcgttggcca tgatggcaaa cgggcagctt tcggtgctgg ctacggtgac
     2401 gaagtcggac gggatagcgg taactttatc gccgtggctc atccagacgt ccagcagcgg
     2461 tttaccatct gcggtcagag catcttcgat accgcgaacc agtgcgctgt cgtttacgac
     2521 ttcaacctgc gcgtagccaa attcacgttc attagaagcc tcaacgtgac cgcccaactg
     2581 catcgccatg gtctgcatac catagcaaac gccgaatacc ggtacgcctg cttcaaagac
     2641 atactgcggc gcacgcgggc tgttttcttc agtagtactt tccgggccgc cggaaagaat
     2701 aatgccgctt ggattgaagt cacgaatttg tgcttctgtc acatcccacg cccacagttc
     2761 gcagtaaacc cccagttcac gcacgcggcg cgcaaccagt tgagtatact gggaaccgaa
     2821 gtccagaatg aggatgcgat gcttatgaat gttttccgtc attgacgctt attccgaggc
     2881 aagtgaaaca gataatataa atcgcccgac atgaagtcgg gcgaagagaa tcaggagccc
     2941 agacggtagt tcggggactc tttagtaatg gtcacgtcgt gaacgtggct ttcctgaata
     3001 cccgcaccgc tgatacgtac aaactccgct ttagtacgca gttcgtcgat agtaccacag
     3061 ccggtcaggc ccatacagga gcgcaggccg cccatctgct ggtgaatgat ctctttcagg
     3121 cgacctttat aggctacgcg accttcgata ccttccggca ccagtttgtc ggcagcgttg
     3181 tcgctctgga agtaacggtc agaagaacct ttggacatcg cgcccaggga acccatacca
     3241 cggtaagatt tgtaagaacg gccctggtag agttcgattt cacccggaga ttcttcagta
     3301 cctgccagca tggaacctac catcaccgcg cttgcgccag cggcgatagc tttagcgata
     3361 tcgcctgaga aacggatacc gccgtcagcg ataaccggaa tgccggtacc ttccagggct
     3421 tctactgcat cagcaacggc ggtgatctgc ggaacaccga caccagtcac gatacgcgtc
     3481 gtacagatag aaccagggcc gatacccact ttaacggcac tgcaaccggc ttctgccagc
     3541 gcgcgggcac ctgcagcagt tgccacgttg ccgccgatga tttgcaggtc aggatattta
     3601 gcgcgagttt cacggatacg ctgcagaaca ccttcagagt gaccgtgaga ggagtcgatc
     3661 agcagaacgt caacgcctgc ggcaaccagc gcatcaacac gctcttcgtt accagcgccc
     3721 gcgccaaccg ctgcacctac acgcagacgg ccttgttcgt ctttacacgc gttcggttta
     3781 cgttccgctt tctggaagtc cttaacagtg atcatgccga tcaggtggaa ttcatcatca
     3841 accaccagcg ctttttcaac gcgtttttcg tgcatttttg ccagcaccac ttcacgggct
     3901 tcgccttcac gcacggttac cagacgctct ttcggcgtca tgtaaacgct aaccggctgg
     3961 ctcaagtcgg tcacaaaacg cacgtcacga ccggtgataa tgccgaccag ttcgttttct
     4021 tcggtaacaa ccgggtagcc agcaaagccg ttacgctcgg tcagttcttt cacttcacgc
     4081 agcgtcgtag ttggcagtac ggtctgcgga tcggttacca cgccagattc gtgttttttc
     4141 acacggcgaa cttcttctgc ctggcgttca atggacatgt ttttgtggat aaagccgata
     4201 ccgccttcct gagccagagc aatagccagg cgcgcttccg ttacggtatc cattgctgcg
     4261 gaaagcatag ggatattcag acgaatagtt ttcgtcagct gagtgctgag gtcagcagta
     4321 tttggcagaa cggtagaatg agcaggaacg aggagaacgt cgtcaaacgt cagagcttct
     4381 ttagcgatac gtagcatggg caatatctcg accagagtgg ttaataaata ttgccgcggc
     4441 attatacaga gcgtaaccga ttgcatctac ccctttttgc aaaaaatgct tgctatcccc
     4501 ggagggcggg ttactatcga ctgaataacc tgctgattta gaatttgatc tcgctcacat
     4561 gttaccttct caatcccctg caatttttac cgttagccgc ctgaatcaaa cggttcgtct
     4621 gctgcttgag catgagatgg ggcaggtgtg gatcagcggc gaaatctcta attttacaca
     4681 accagcctcc ggtcactggt actttacgct taaagacgat accgcgcagg tgcgctgtgc
     4741 gatgttccgc aacagcaacc gccgggtcac cttccaccca cagcacgggc aacaggtttt
     4801 agttcgcgcc aatattacac tctacgagcc gcgcggcgat tatcaaatta tcgtcgaaag
     4861 tatgcagccg gccggtgagg gattgctgca acagaagtac gaacagctta aagcgaagtt
     4921 gcaggctgaa ggtttgttcg atctgcaata caaaaaatca ctcccctccc ctgcgcattg
     4981 cgttggtgtg atcacctcaa aaaccggtgc tgcactacat gatattttgc atgtgttaaa
     5041 acgtcgcgat ccttctctgc cggtgatcat ctaccccacc gccgtacagg gggatgatgc
     5101 gccggggcaa atcgttcgtg ccattgaact ggcgaataag cgcaacgagt gtgacgtgtt
     5161 gatcgttggc cgtggcggcg gttcgctgga agatttatgg agctttaacg acgaacgcgt
     5221 ggcgcgggcg atttttgcca gtcgcattcc gatagtcagc gccgtcgggc atgaaaccga
     5281 tgtgacgatt gccgactttg ttgctgatct gcgtgcgcca acgccgtcag ccgccgctga
     5341 agtggtgagc cgaaatcagc aagaattgct acgtcaggtg caatctgccc gtcaacggct
     5401 ggaaatggcg atggactatt atctcgccaa tcgcacgcgg cgctttacgc agatccatca
     5461 ccgcttgcag caacagcatc cgcagctccg gctggcacgc cagcaaacta tgctcgaacg
     5521 tctgcaaaag cggatgagct ttgcgctgga gaatcagctt aaacgcgcgg gtcagcaaca
     5581 gcagcgatta acacggcaac tcgtccacca aaatccgcag tcgcgtattc atcgcgcgca
     5641 aacgcgcatt cagcaactgg aatatcgttt agcagaaacc ctgcgcgcac agcttagcgc
     5701 cacgcgcgaa cggtttggta acgcggtaac acacctcgaa gcggttagcc cgctgtcaac
     5761 gctggcgcgc ggttatagcg tcaccagcgc cgccgatggc gcagtgttga agcaggtgaa
     5821 gcaggtgaaa gtgggcgata cgttgaccac gcgactggga gatggcgtgg tcgtcagtga
     5881 agtgagtgcg gtgacgaaaa cccgcaagcc gcgtaaaaaa acctgatagc ccgttgcgta
     5941 tcaggctttc ggaacctcat attgataggg tgggcaaggt tattggaaac aggttgcaaa
     6001 ccccggcgtt ttcgcaggct gaatgccaca gttgagactg ccagagcctt cctcaacacc
     6061 acaatagaac gatccagcct tatcagtaga taaatacggg aagcttatcg atgacggcca
     6121 tcccaatgtg ctaaagccgg agtgtaatgt ttgtaattcg gcactgctcg gcacgttatt
     6181 catactaccg cactgccgtg cgacatccca tttagtggaa tctataacat gtgccagcgc
     6241 ccaggtttca ttattcacat tgggcgttgc tgctgcggta acccctgacg gcaactccga
     6301 ttttaatagc gggcgatgca gcatcttgcc acttaccgtg gcggtatccg ccatatgacc
     6361 ccagaaatac gccgtggcga cattaggact ggtcggcacg gtgaaaatcg tgtccagcgt
     6421 cgccgttatt gtggcgttat ccgtcagcgt caccgtcaac ggcgttttat atcctggcgt
     6481 attatcctga ccaacggtaa aacttgctgt accgtctgct ccagtaactc catcgattgt
     6541 attcccgcta tcagccaacg tgactgccgc accggatgag ggttgtacag tcaaatcgtc
     6601 cattgtatcc acatcgccat acaacgtcgc cccgctatta cgcggcgtag cgtcaccacg
     6661 tttgaggata aacgaaatat tcggcactgg tttcccgaca ctgtctttca cggtaacgac
     6721 aacgggaacg gcgtcgcctt ttttcacctt cgccgcctgc gtactgctat ccagtgccgt
     6781 cgatgtcaac gtgacgctgg agacagtcgc acgcgggtta accaggcacc cttgatacgc
     6841 ggtattcacc gtcccccacg ctgaaccatt attcagaccg actacctgcg gattactgcc
     6901 tcccagccag ttatccagcg cccaccatgt atactgcgta gaccaaccga tttgtgtcgt
     6961 cagcgtgttc cccggatagc gagcatagag cgattctaaa tcatcactca atggccgacg
     7021 tgccgcttca caagggctga gattgctgtc agccctctgc gttgcgttaa acacgggcca
     7081 gacttcattg ttactattga ccttgctaac ccctgatgtc gacggcagct ctttccacaa
     7141 tttaggtctt tcaaagacca caccgttttc tgccgttacc gtatccggca tatgacccca
     7201 gtaggccgcc attgcggaat cagggctggt gacaacggtg aagatcgcat caaggacagt
     7261 ggtggtttcc gtatcacgtg ccaggatggc ggtaaacggt gttgccaggc cgacagtatc
     7321 aggctgttca acattgaata tcgccgtacc atcgctgccg gtaaaaccaa gccattgcgt
     7381 gccgtgtgag gcgagagtga atgttgccga cacgggtgcc agttccgtca ataccatcac
     7441 atttgctgcc gtgtttacaa tggttcctga gcggctcatc gcattatcac gcctgagcgt
     7501 tatcgcttcg cccgccagcg ggttgccatc acgatctttg accataacca caattggcaa
     7561 tgggttatct tttttcactg acgcgctagg tcggccgcca ttgctgaggg ttctggcgtc
     7621 atccagtgcg gatgacgtca atgttacgct aacgcctgcc ggatgcggct gcgccagaca
     7681 gagcatcagt gcgttggttg cagaattctt cgcgatagct cctgttctca ggctgacagt
     7741 ctgataagac cacgttgttg tacccagtat ggccatatca taagcccacc agttacccgc
     7801 attcatcggt aacccaaggt cagtcattat tgcgccgttc ggatgatcac tatacaaatc
     7861 caacagttcc ttactgcgcg gttgattctc gagtgggcaa tctgtagatg ttaaattttg
     7921 caagttgctc atatacgacc aaacttcgcc gttagaacta atcgataagc ctgagctggt
     7981 ttcggttttc agcaggggtc gtttgaaggt gacgcccgcc gaactggtaa aggtgtccgg
     8041 catatgcccc cagaatttag cactcggtga atctgggctg gtaatcaccg tgtagataac
     8101 atccagcgtg gcgactttcg ttgtatcacg cgccagcgtc acggttaacg gcgtcgccaa
     8161 cccggtagta gagtactgcc caacctcaaa ggtggctgtg ccatcactgc ccgtcgttgc
     8221 gagagttttt gctccacttg ctgtcaacac gctatcccta ctcaccggaa ccaatgccgt
     8281 tacggtaaga tcgtctgcac tcgtgttaac ggttgctttc gcgcgatcca gcgtcgtttc
     8341 acgcgaaagg gtaaagtcgg catatggcat cgggttcccg gcgctatcac gcacggtcac
     8401 cgtcaacgga atcagctcac ctttcttcgc tacagcgctc ggtcgtccat cattgctgag
     8461 cgattttgct gcatcctgcg ccgttgaggt aagctctatt gtgactgctt ccgtatgagg
     8521 ctctaccaaa cacatcacta tcgccgtata gctggacgat gcctgaatca actgaccatt
     8581 tcccggaaca aacgcctggt tactccagct accgcccgct accgccatca tgtcataatc
     8641 ccaccagttc cctgcagcag tcggcaagcc gagcaccgtt gccagttgac cattgggata
     8701 atcgacatac agcccctgca tatcagtcgt cagcggttga tacgcctcat cacacccagc
     8761 cttgctgacg tccgctttct cagtcgcggt caggagcgac cagacttcat tattggccgt
     8821 tttcgccgta ccgccagaga gttcggcttt gaggaaaggc cgtttgaagg tgacgccttc
     8881 gctgctggta aaggtttccg ccatatggcc ccaattggtc gccagcggcg tatccgggct
     8941 ggtggtgacg gtaaagatca ccgacaaggt gtccgacgac acgccagtat cgtttaatag
     9001 agcataaaag tcggttctta acccagtgga agcatcctgt cggagcgtca tcgtagtggt
     9061 tccatctggc cctgttacac catacaggat ggtcgttgca ctggtatcca ccgtcgcacc
     9121 cgccgcagtc gtgactgaca ctttctgcga tgacgtcaca ttctgacgat tgatcgagtt
     9181 cgcgtgtttc agggcaaacg gcgtattgcc gactaaattc ccctgcgcat ctttagtcga
     9241 aatgcggatc gacacctcat cccccttctt cgctttgacg gcgctaagtg tgctgtcaat
     9301 gctgacaaac tgcccggggt ctgaggcttc cagcgtaatc tggctggcag tcatcaacgg
     9361 cgttgtacgg caggtttgta acgccagcac ggtggatacg atttgcgaaa ctgcgccaga
     9421 atttagttgc accacattgt agtaacggct ccctgtttgt gtattactcg gggtattggt
     9481 gagataactc atatttaccg gccatccctg taccgtattc atcgcattac ctctattggc
     9541 gtcatacaaa gacttcagac catccagcgt tggcatataa tctgtaccac acccattcat
     9601 gctggtgtca gccgactgag tcgaggtgaa tcttgcccaa ctttcaccgt tctcaaacgt
     9661 gctatatcgt gcatagttgt tggtaagctc gccaaacagc aacggtcgct tataaaccga
     9721 accatcggag gccgtcaccg tttcggccat atgtccccaa aaattcgctt ttggcgaatc
     9781 cgggctggtc acgacggtaa agaccaccgg tagcatcgcc ttgacgctgc tggacgtatc
     9841 cagttttgcc gtgagatcgg ttcttaaccc ggtactgtta tcctgtttta gggtaaatgt
     9901 cgttgacccg tcggcaccgg tcacgccgta aatggtttca ctcgttgact ggaaattatt
     9961 tcgcgtattt ccccacgcat ccgtcaccgt cagcgcgccc acactcatcg tcgccgatga
    10021 attagcccga cttacactgt tggctcgcgt caggataaat gccgtattgc ccacaaggtt
    10081 gccttgcgca tctttggtga tgacgcgcac cacggattct tcgctttttt tcgcttttac
    10141 cacgttatag gtggtatcca gcgtcaccgg atctgccgct tccagcagga tttggctcac
    10201 cgcggctatc ggcgcggtct ggcaggtcag cagctccagc gtcgttgatg acactgccgc
    10261 actcgtgcca ttgctgagat ctaccgcctt gtagttacgc tgctccatgc tgccgttgtc
    10321 ggaagtgttg gataaatagc gcgttgccac cggccagccc tgcacggttt tcatggtgtt
    10381 gcccgcatta ttagcaaaca gtgacgtcag accggcctga gtcggaatat agttggttcc
    10441 acagccgctg tacgtcgtgg aaaaacttgc ataattaatg ttaaacaacg cccagttttc
    10501 attgtcttca gacgttgatg ttcgcccagt ctgataggcc agttccttca acaacagcgg
    10561 gcgcttaaat accgtaccgt ccgcagcagt caccgtttcc ggcatatgtc cccacatttt
    10621 tgccaggcgg ctatccgggc tggtcaccac cgtaaagatt gtgtcgatgc tggatttttt
    10681 cgtcgcatca gagtaaagca ttgccgtcag cgccgttttg gtgccgtgag tatccgggcg
    10741 ggtaatattg agaattttgc tgccgtcatt gtcggtcatg gcggtataca ccgttgcagt
    10801 gtcgttcaac gtggtttcat ctgccagccc gccattgact accaccggcg caaccagcgc
    10861 atcgccgctg cccgccacat gtttttcgcc gctacgggta tagccatcac cgcgattgag
    10921 ggtaaatggc atatccgcta acgcgttgcc ctgtgcgtct tttaccgtca ctttaacctg
    10981 tagcgtctcg ccttttttca gcgttgccgc gctattcccg gtattccatt gcgctttatc
    11041 aaccacctcc agcgtaatgc tgctggcagg tttgttcgcc gtcttcaggc aactgacgta
    11101 aaccggcgta tcgccgccaa tagtttgtgc gccatcgttc agcgctatcg tgaaaaagtg
    11161 tggagtttta tctgccggtg agttgctcca gtaaggctga cgctgtgttg gccaaccgtg
    11221 cgccgtttgc accgcgttac cactgttggc gttatacagc gcacttagct gggaacgacg
    11281 tggcaacata ttggtgtcgc agccaccttt actggtactg gacgtattgg cctggctcac
    11341 gcgcgcccag atttcgttat tttcggtcag ggttccgtcc gggctggcga cttccgacgc
    11401 cagtttaggc cgggtaaagg ttgatgaatc aacggtgatc gtttcatcca tatggcccca
    11461 catctgcgcg ccggtaacgt ccgggctggt tggggtagta aaaataacgc tgtagttaac
    11521 ggtattcggc aggtaggaat ttaccggcgt tacgctcagc accgttttta gcccgacgcc
    11581 ctgtggttgc ttaatctcga ccgtcgccac gccccgggca tcggttattc ccgcataaac
    11641 cttcgatgac tgtgatgtgc cgtattgcgt gttatccatc gtgattgcac cactgctcgg
    11701 gtcatcaaac ccgctcacct gccccgctcg gttatacccc atacctttgg taatggtgaa
    11761 tgcagtaaag ggtacaggga tattattgag cgagttaatc gtacgcacgg tcatcacaat
    11821 ggtgtcaccc actttggctt gcgcttgggt gccaacagac acatcatgat cggtgctgac
    11881 ttcaacattc gctaccatct cattacccga acaggagagg taattcggct taaagccgga
    11941 atagctgtcg acgctcccgt tccccagatc caccgatgaa tgcgtggctt gctccaccgc
    12001 gcttaaatag ccctgttgtg cagtgggcca gccatattca gtaccaatcg cgttaccggg
    12061 atgggctgaa aacaggcttt ccagtgagct ctggcgcggt atatgaccga cgccgcattc
    12121 cgcctgcata ctggtgtttt gatcgtagag cgcccagtct tcgttatttt cccgcactgt
    12181 tcccaattca tgctcggttt catccgccag caacgggcgt ttatacaaac tgccggactc
    12241 aataatgccg cgcatatggc cccacatccg cgctttgtcg ctgtccgggc tggtgattac
    12301 ggtaaaaatc acatctttgg catctgtggc gttaaaatcg ctgcgcattc ttgccgtgat
    12361 atgggttttc acccccgcgc cgccgggctg gctcagggtg agcgaagcct ggccgttggc
    12421 gtcggtcatg ccttcatagg tatgtgcgtc gacctggcga aaattactgc cgccagcaat
    12481 ctgtaccgga tgggcttccc aggcagtgtc ggtttgattc ttacgattga cgccatcatc
    12541 cagatgtagc gagaagtagt aatacgccag cggttgatcg ttgtttttcg tgtcggtgat
    12601 ggccagacgc atcgtcgcat cctcgcccac cttcgctttc atcgcctgcg ccgtgtcatc
    12661 atagttttct ggcgtgagga cgattttcgg ctcgacgtca ggcgcgggct tatccacgca
    12721 gctcacgagg aacgtggtgc tgtccggctt ctgcgtttcg ccacggttag agacatctgc
    12781 cgcatggtgc tgcccggtaa gccccgccag cgatgaccag taataatcgc cctgcatcgg
    12841 ccagccgagc acagtctgta ccgtggaggg aatcaccgtc gccagtgcgc cgaagtggcg
    12901 catgcccggc agcacggtgc aatggctatc tgcgccgctc caggtaaagg tggaccaggt
    12961 ttcattgtga tcgacgaccg tggcattttc gttactgact tccgccgcca gttttggtct
    13021 actgaacgtg taaccatggg cttccaccgt ctccgccata tggccccaca tcgtggcctg
    13081 cgccacgtcc gggctggtga gcacggtaaa gatcaccgcc gcctcactgg tttgtgcaat
    13141 gccggaaatg ccgactacca gcggcgtttt aacaccgggg ccgttaggct gggtcaccgt
    13201 gatggttgcc gtaccgttag catccgatgt gccacgatac tcggttaccg tcgtcgttaa
    13261 ctcggtgcta tccagcacca ccggcgcggt attatttacc gcgccctgac ggttttgggc
    13321 atctttacgc ttgataataa atggaatatt acccgcgacg ttaccctgac aatctttggt
    13381 ggtgacggtc agcgtgatag tgccgccaac ggtggttttt gccgcgccga gcgtttcgtt
    13441 ataaccactg gcgctcaccg aaattaaatc gccctgcgcg ggcgcgatat tcagcgtcac
    13501 tttctggtca gccagcacca ccgtcccctc atagggataa ccggtcgagg tcagcagata
    13561 aagcagtgga atggtctgct gcgcatcaat ctgtggccag gccaccagcg aacgcccggc
    13621 cagaccttgc gcgcaaaagc tgcggttggc gatgggctgt ggattgagcg gcgtatccgg
    13681 cgtggcggca tccgcccaca ccagactgac ggtcggcggc gtctggtttt cccagcgcag
    13741 cgcaggtggt gtagaaaact ggtcgccttc actgtcctga ggattagcaa ctatcaggtt
    13801 agcagcgtca tcgtcaacgc taaattcatt cggtttagca gaaaacgcca catcatgaga
    13861 tgcagcagga tcaagctgaa cgcttccctg ataaacgggt acgctggcgc tatcggcaat
    13921 aggaactgta ccgttaaact cgcctgtaga accaggatat gtctgccatt ccacctctgc
    13981 ccgtgcaaag tcagacagca taaaggctgc caacatgccc agaagcacaa tgatttttcc
    14041 tggtttcaga cataccgaca tgtagaaaac tccagcatga taaacaaaac gtcaacccgt
    14101 tttgcttaac gcgtcgctaa gcaaaacagg cggaaaacag cattcccata cgggaatgct
    14161 gttgtgcgca ttagtggtgt ttgtaaatga tagacagcgt atagccctga acgccgtcgc
    14221 cgtttgccgg gattgcaagg ccggtcttac catcacggtc actaccgtta aggctggttc
    14281 ttgcttgttc gttagtttgc ggaataacaa tgtcattgtt atcgaagctg gcgttggcaa
    14341 taccgcccgc cgccgcaagt tgtttactgt taccgttaaa gacccacgca aagtcgtagt
    14401 tagcggtaac gtccacatcc gtacccgcgt cgtatttacc gttctggttt tcatccgagt
    14461 acagtttggc cacatacgtt ttgttaagcg caatggctgt attaccaccg ataaggttgg
    14521 tactggtgtc tttctcgaag atggcaactt tcaggttctg gttaacaacc ggaccaggat
    14581 cgacattatc cgtatcgctg ccgccaccgc tggccgtaga aatatccagc agagtcagcg
    14641 aagttccctg gaagggatta ccggtctgag tgataggctg gatttcaaca ccgatatagc
    14701 gtccacgatc gccatcggtg attttataac tggtttttcc tgccgccgca gtcaactccg
    14761 ttttaccgcc acccgcatta tcactgtagc tgtaccattt gatggtcgcc gtcgttttcg
    14821 catcaatgcc tgcggaacca tcatcgatat ccccttcggt atcgccgata tcccaaccca
    14881 gcgtgatggt atcccctgag taaaggcgtt tatcagactc gctacctgca ggacggatac
    14941 ctttactatc agatgtcact ttgatgtgat ccgcatcttc cgagttaatc gtagcattcc
    15001 ccccttcgcg atagagccag ggtatggtcc cctcgaattc agctgtcgtt ccactggcga
    15061 cccaggcagc atcttccgcc atagcgactg gtacggcaca gtatccagcc agcatcagtg
    15121 ccagcgcgac ttttgtcagg ccacgtttca tatgaggttt cataaacact ctccttggtt
    15181 aacattcagc ggtggcccga tcagccatgg cgctgttcct atgtcttgct gtaattgacc
    15241 tgcaaaccgt acccttgcac accatcggtt acctgggcac taaacttcgc tgccgcttcc
    15301 ttgttcgttg ccgggatctc gatatcgacg ttagtggtat tggcttgcgc gccagtattg
    15361 ccatttacgc tatgccccgt gaattcccat tggtagtcat actgtttcat ctcttcgtca
    15421 gtgagctttt cagatagctg gaacacaccg tctttgttag cgtcgcgcca caacacaaag
    15481 cgatatcggc tattgagggg caacggttct ttattgccga taagattcac ggatttagcc
    15541 gcaccaacgc gatagataaa ggcattcagc ccgccgatgt cagcccccag gaacgttacg
    15601 tcaacatagt tactgtcatc atagggggca gctttcgcct tccaacggaa ggtgccaggc
    15661 aacgtactgg agagcgtaac ggtccccttt tctttatctt gtttggcgat aaagacacag
    15721 ggagtctcgg tagtgcaggg gttattcgtc agcgtcactt ttttatcgtt ggcatcaaac
    15781 agctgcgggt tgagattatc ctgcggcaag acattaccgt tgcggtcagt gttgtcgcca
    15841 aagctatcag taatatcgag atccatcacc acaccatcta caccatcagc ctgtgcactg
    15901 gcttcgtggc tgtggttgtt ctcttccggc acgttcgcga tgttattaac aatcagctca
    15961 attttacggt cctgctgaac ggtgatttcc accacgcctg agttctgtcg gttgcccttt
    16021 tcatctttca gcgccaggcg aacccgccag gtattgagtt ttccttgttc ggctttgtca
    16081 gagctgtact gccagacggg cagcaccagg ttccagtggt tattgttgcc ctctgtcagg
    16141 gtattctctt tgataaccgg catcaccagc gtgcgggcgg cattgttttt cgcctgccat
    16201 gacgcagcga caattttgta gtggtggttg acaagcgcgt taatggtcca tttacatttt
    16261 tccagcccga tagcttcttc tggcgtatct ttaatcacgc actcgggatg ttcattcatc
    16321 gcatcagctt tcagccacaa agtgacatcg aggggatctt tctctttata ttccagaaca
    16381 ataaagttgt tacgttcaac aaagtcgtga cggctgccca tgagtgagtg agccgcgctc
    16441 actttgtcag gatcaagctg gtctttgagc gggacgccaa actggtagtt aagcgttgcg
    16501 ttgaccgtgg tatcggtgtt atcaccggtt cctgctttga agtccgttcc tacagcaatg
    16561 agcggaacag gctggtattt cacgcctaac gtgacagcga agggatcttt ctctagattg
    16621 tcggtaccaa atagcgcaac ttcatccccg taataatgtt cgaaaacgat tttaccgcca
    16681 agctgcggat aagcgggaag ccaggcttcg gcacgaatat cccaaccgct cgcagggcgt
    16741 tcttcataaa agtcgaaatc ttcagagtct ttccagtcag aaagcggatg ataatagtta
    16801 ccagaaaatt ttaaataatc agtccacgct tcagcgccta tacctaaacg gcgatgtcca
    16861 cgggtaaaat cataatcata aaatatattt ccacccaata accatttatc aaaattatgt
    16921 cttacaccaa gccctatatt tccgatcgta cgatcttctt ttcgttgcgc agaaaattgg
    16981 ctgaaataaa cagtggtttg attatcgtac cagggaacaa aataatcgat ggaactgcca
    17041 tccagatcgc cgccctgacc aatggagagg ttcgaacgga ccttaccgta gggagagagt
    17101 gtgtcttcaa tataagattg tgcggttgag gtgatttgac tttgcagata gcttctggcc
    17161 tgagattcca gcgcctcagg ggtcaagttt tcaaaccctt gcgtggcgga gttgatgacg
    17221 taatcggctc cgcgctcttt cagcgattta cccttgttgg tttgttcatc ctgttgtgct
    17281 gcctggctac ctaaatcagg taacgattcg ttttgcttaa catttatctc tggtgccgcg
    17341 aaagcagcac cagagataaa tgttagcata atacagcg
//
LOCUS       DAHHTG010000094        17127 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.094, whole genome shotgun sequence.
ACCESSION   DAHHTG010000094 DAHHTG010000000
VERSION     DAHHTG010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17127)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17127)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17127
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.094"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            331..1356
                     /gene="purR"
                     /locus_tag="NBI74_003192"
     CDS             331..1356
                     /gene="purR"
                     /locus_tag="NBI74_003192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor PurR"
                     /protein_id="HCD1933478.1"
                     /translation="MATIKDVAKRANVSTTTVSHVINKTRFVAEETRNAVWAAIKELH
                     YSPSAVARSLKVNHTKSIGLLATSSEAAYFAEIIEAVEKNCFQKGYTLILGNAWNNLE
                     KQRAYLSMMAQKRVDGLLVMCSEYPEPLLAMLEEYRHIPMVVMDWGEAKADFTDAVID
                     NAFEGGYMAGRYLIERGHREIGVIPGPLERNTGAGRLAGFMKAMEEAMIKVPESWIVQ
                     GDFEPESGYRAMQQILSQSHRPTAVFCGGDIMAMGALCAADEMGLRVPQDVSLIGYDN
                     VRNARYFTPALTTIHQPKDSLGETAFNMLLDRIVNKREEPQSIEVHPRLIERRSVADG
                     PFRDYRR"
     gene            complement(1353..2285)
                     /locus_tag="NBI74_003193"
     CDS             complement(1353..2285)
                     /locus_tag="NBI74_003193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCD1933479.1"
                     /translation="MWSEYSLEVVDAVARNGSFSAAAQELHRVPSAVSYTVRQLEEWL
                     AVPLFERRHRDVELTAAGAWFLKEGRSVVKKMQITRQQCQQIANGWRGQLAIAVDNIV
                     RPERTRQMIVDFYRHFDDVELLVFQEVFNGVWDALSDGRVELAIGATRAIPVGGRYAF
                     RDMGMLSWSCVVASHHPLALMDGPFSDDTLRNWPSLVREDTSRTLPKRITWLLDNQKR
                     LVVPDWESSATCISAGLCIGMVPTHFAKPWLNEGKWVALKLENPFPDSACCLTWQQND
                     MSPALTWLLEYLGDSETLNKEWLREPEETPATGD"
     gene            2398..3609
                     /locus_tag="NBI74_003194"
     CDS             2398..3609
                     /locus_tag="NBI74_003194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bcr/CflA family multidrug efflux MFS
                     transporter"
                     /protein_id="HCD1933480.1"
                     /translation="MQPGKRFLVWLAGLSVLGFLATDMYLPAFAAIQADLQTPASAVS
                     ASLSLFLAGFAAAQLLWGPLSDRYGRKPVLFIGLTIFALGSLGMLWVENAATLLILRF
                     VQAVGVCAAAVIWQALVTDYYPSQKVNRIFATIMPLVGLSPALAPLLGSWLLVHFSWQ
                     AIFATLFAITVVLILPIFWLKPTTKARNNSQDGLTFTDLLRSKTYRGNVLIYAACSAS
                     FFAWLTGSPFILSEMGYSPAVIGLSYVPQTIAFLIGGYGCRAALQKWQGKQLLPWLLV
                     LFAVSVIATWAAGFISHVSLVEILIPFCVMAIANGAIYPIVVAQALRPFPHATGRAAA
                     LQNTLQLGLCFLASLVVSWLISISTPLLTTTSVMLSTVVLVALGYMMQRCEEVGCQNH
                     GNAEVAHSESH"
     gene            3900..5048
                     /gene="cfa"
                     /locus_tag="NBI74_003195"
     CDS             3900..5048
                     /gene="cfa"
                     /locus_tag="NBI74_003195"
                     /EC_number="2.1.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane fatty acyl phospholipid synthase"
                     /protein_id="HCD1933481.1"
                     /translation="MSSSCIEEVSVPDDNWYRIANELLSRAGIAINGSAPADIRVKNP
                     DFFKRVLQEGSLGLGESYMDGWWECDRLDMFFSKVLRAGLENQLPHHFKDTLRIAGAR
                     LFNLQSKKRAWIVGKEHYDLGNDLFSRMLDPFMQYSCAYWKDADNLESAQQAKLKMIC
                     EKLQLKPGMRVLDIGCGWGGLAHYMASNYDVSVVGVTISAEQQKMAQERCEGLDVTIL
                     LQDYRDLNDQFDRIVSVGMFEHVGPKNYDTYFAVVDRNLKPEGIFLLHTIGSKKTDLN
                     VDPWINKYIFPNGCLPSVRQIAQSSEPHFVMEDWHNFGADYDTTLMAWYERFLAAWPE
                     IADNYSERFKRMFTYYLNACAGAFRARDIQLWQVVFSRGVENGLRVAR"
     gene            complement(5088..5729)
                     /gene="ribE"
                     /locus_tag="NBI74_003196"
     CDS             complement(5088..5729)
                     /gene="ribE"
                     /locus_tag="NBI74_003196"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HCD1933482.1"
                     /translation="MFTGIVQGTAKLVSIDEKPNFRTHVVELPDHMLDGLETGASVAH
                     NGCCLTVTEINGNHVSFDLMKETLRITNLGDLKVGDWVNVERAAKFSDEIGGHLMSGH
                     IMTTAEVAKILTSENNRQIWFKVQDSQLMKYILYKGFIGIDGISLTVGEVTPTRFCVH
                     LIPETLERTTLGKKKLGARVNIEIDPQTQAVVDTVERVLAARENAMNQPGTEA"
     gene            5944..7317
                     /gene="mdtK"
                     /locus_tag="NBI74_003197"
     CDS             5944..7317
                     /gene="mdtK"
                     /locus_tag="NBI74_003197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MATE transporter MdtK"
                     /protein_id="HCD1933483.1"
                     /translation="MQKYISEARLLLALAIPVILAQIAQTAMGFVDTVMAGGYSATDM
                     AAVAIGTSIWLPAILFGHGLLLALTPVIAQLNGSGRRERIAHQVRQGFWLAGFVSVLI
                     MLVLWNAGYIIRSMENIDPALADKAVGYLRALLWGAPGYLFFQVARNQCEGLAKTKPG
                     MVMGFIGLLVNIPVNYIFIYGHFGMPELGGVGCGVATAAVYWVMFLAMVSYIKRARSM
                     RDIRNEKGTAKPDPAVMKRLIQLGLPIALALFFEVTLFAVVALLVSPLGIVDVAGHQI
                     ALNFSSLMFVLPMSLAAAVTIRVGYRLGQGSTLDAQTAARTGLMVGVCMATLTAIFTV
                     SLREQIALLYNDNPEVVTLAAHLMLLAAVYQISDSIQVIGSGILRGYKDTRSIFYITF
                     TAYWVLGLPSGYILALTDLVVEPMGPAGFWIGFIIGLTSAAIMMMLRMRFLQRLPSAI
                     ILQRAAR"
     gene            complement(7358..8614)
                     /locus_tag="NBI74_003198"
     CDS             complement(7358..8614)
                     /locus_tag="NBI74_003198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA repeat-containing protein"
                     /protein_id="HCD1933484.1"
                     /translation="MGSDAKNLMSDGNVQIVKTGEVIGATQLTEGELIVEAGGRAENT
                     VVTGAGWLKVATGGIAKCTQYGNNGTLSVSDGAIATDIVQSEGGAISLSTLATVNGRH
                     PEGEFSVDKGYACGLLLENGGNLRVLEGHRAEKIILDQEGGLLVNGTTSAVVVDEGGE
                     LLVYPGGEACNCEINQGGVFMLAGKASDTLLAGGTMNNLGGEDSDTIVENGAIYRLGT
                     DGLQLYSSGKTQNLSVNVGGRAEVHAGTLENAVIQGGTVILLSPTSADENFVVEEDRA
                     PVELTGSVALLDGASMIIGYGADLQQSTITVQQGGVLILDGSTVKGDSVTFGIGNINL
                     NGGKLWLITDAATHVQLKVKRLRGEGAICLQTSAKEISPDFINVKGEVTGDIHVEITD
                     ASRQTLCNALKLQPDEDGIGATLQPA"
     gene            8922..8998
                     /locus_tag="NBI74_003199"
     tRNA            8922..8998
                     /locus_tag="NBI74_003199"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8956..8958,aa:Val,seq:gac)
     gene            9003..9079
                     /locus_tag="NBI74_003200"
     tRNA            9003..9079
                     /locus_tag="NBI74_003200"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9037..9039,aa:Val,seq:gac)
     gene            9188..9493
                     /locus_tag="NBI74_003201"
     CDS             9188..9493
                     /locus_tag="NBI74_003201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monooxygenase"
                     /protein_id="HCD1933485.1"
                     /translation="MATLLQLHFSFNGPFGDTMAEQLKPLAESINQEPGFLWKVWTES
                     EKNHEAGGIYLFTDEKSALAYLEKHTARLKNLGVEEVVAKVFDVNVPLSQINQAKLA"
     gene            9619..11223
                     /locus_tag="NBI74_003202"
     CDS             9619..11223
                     /locus_tag="NBI74_003202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-NAD(P)-binding protein"
                     /protein_id="HCD1933486.1"
                     /translation="MKTIAIVGAGPTGIYTLFSLLQQQTPLSISIFDQADEAGIGMPY
                     SDEENSKLMLANIASIEIPPIYCTYLEWLQKQEASHLQRYGVKKETLHDRQFLPRILL
                     GEYFRDQFLRLVDQARQQKFAVAVYESCQVTDLQITNAGVMLATNLNLPSETFDLAVI
                     ATGHVWPDEEEATRTYFPSPWSGLMEAKVDACNVGIMGTSLSGLDAAMAVAIQHGSFI
                     EDDKQHVTFHRDNANEKLNITLMSRTGILPEADFYCPIPYEPLHIITDQALNAEIQKG
                     EVGLLDRVFRLIVEEIKFADPDWSQRIALESLNVDSFAQAWFAERKQRDPFDWAEENL
                     QEVERNKREKHTVPWRYVILRLHEAVQEIVPHLNEHDHKRFSKGLARVFIDNYAAIPS
                     ESIRRLLALREAGIIHILALGEDYEMEINESRTVLKTEDNSYSFDVFIDARGQRPLKV
                     KDIPFPGLREQLQKTGDEIPNVGEDYTLQQPEDIRGRVAFGALPWLMHDQPFVQGITA
                     CAEIGEAMARAVVKPASRARRRLSFD"
     gene            complement(11235..12047)
                     /locus_tag="NBI74_003203"
     CDS             complement(11235..12047)
                     /locus_tag="NBI74_003203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1933487.1"
                     /translation="MIITRADLREWRIGAVMYRWFLRHFPRGGSYADIHHALIEEGYT
                     DWAESLVEYAWKKWLADENFAHQEVSSMQKLATDPGERLFCSQFVRSDDHARLGCCED
                     NARIATAGYAAQIASMGYSVRIGSVGFNSHIGSSGARARVAVTGNSSRISSAGDSGRI
                     ANTGMRVRVCTLGERCHVASNGDLVQIASFGANARIANSGDNVHIIASGENSTVVSTG
                     VVDSIILGPGGCAALAYHDGERVRFAVAIEGENNIRAGVRYRLNEQHQFVEC"
     gene            complement(12051..12836)
                     /locus_tag="NBI74_003204"
     CDS             complement(12051..12836)
                     /locus_tag="NBI74_003204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate reductase cytochrome B subunit"
                     /protein_id="HCD1933488.1"
                     /translation="MNPSQHAEQFQSQLANYVPQFTPQFWPVWLIIAGLLLVGMWLVL
                     GLHALLRARGVKKSATDHSERIYLYSKAVRLWHWSNALLFVLLLASGLINHFALVGAT
                     AVKSLVAVHEVCGFLLLACWLGFVLINAVGGNGHHYRIRRQGWLDRAAKQTRFYLFGI
                     MQGEEHPFPATTQSKFNPLQQVAYVGVMYGLLPLLLLTGLLCLYPQAVGDVFPGVRYW
                     LLQAHFALAFISLFFIFGHLYLCTTGRTPHETFKSMVDGYHRH"
     gene            complement(12833..13501)
                     /locus_tag="NBI74_003205"
     CDS             complement(12833..13501)
                     /locus_tag="NBI74_003205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416186.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HCD1933489.1"
                     /translation="MSFTRRKFVLGMGTVIFFTGSASSLLANTRQEKEVRYAMIHDES
                     RCNGCNICARACRKTNHVPAQGSRLSIAHIPVIDNDNGTQYHFFRQSCQHCEDAPCID
                     VCPTGASWRDEQGIVRVEKSQCIGCSYCIGVCPYQVRYLNPVTKVADKCDFCAETRLA
                     KGFPPICVSACPEHALIFGHEDSPEIQAWLQENKYYQYQLPGAGKPHLYRRFGQHLIK
                     KENV"
     gene            complement(13565..14203)
                     /locus_tag="NBI74_003206"
     CDS             complement(13565..14203)
                     /locus_tag="NBI74_003206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310406.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdhW family putative oxidoreductase system
                     protein"
                     /protein_id="HCD1933490.1"
                     /translation="MNHQDELPLAKVSEIDEAKRQWLQGMRHPVDTVTEPEPAEILAE
                     FIRQHSAAGQLVARAVFLSPPYSVAEEELSVLLENIKQNGDYADIACMTGSQDDYYYS
                     TQAMSENYAAMSLQVVEQDICRAIAHAVRFECQTYPRPYKVAMLMQAPYYFQEAQIEA
                     AIAAMDVAPEYADIRQVESSTAVLYLFSERFMTYGKAYGLCEWFEVEQFQNP"
     gene            complement(14216..16318)
                     /locus_tag="NBI74_003207"
     CDS             complement(14216..16318)
                     /locus_tag="NBI74_003207"
                     /EC_number="1.2.7.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase"
                     /protein_id="HCD1933491.1"
                     /translation="MANGWTGNILRVNLTTGNITLEDSSKFKSFVGGMGFGYKIMYDE
                     VPPGTKPFDEANKLVFATGPLTGSGAPCSSRVNITSLSTFTKGNLVVDAHMGGFFAAQ
                     MKFAGYDVIIIEGKAKSPVWLKIKDDKVSLEKADFLWGKGTRATTEEICRLTSPETCV
                     AAIGQAGENLVPLSGMLNSRNHSGGAGTGAIMGSKNLKAIAVEGTKGVNIADRQEMKR
                     LNDYMMTELIGANNNHVVPSTPQSWAEYSDPKSRWTARKGLFWGAAEGGPIETGEIPP
                     GNQNTVGFRTYKSVFDLGPAAEKYTVKMSGCHSCPIRCMTQMNIPRVKEFGVPSTGGN
                     TCVANFVHTTIFPNGPKDFEDKDDGRVIGNLVGLNLFDDYGLWCNYGQLHRDFTYCYS
                     KGVFKRVLPAEEYAEIRWDQLEAGDVNFIKDFYYRLAHRVGELSHLADGSYAIAERWN
                     LGEEYWGYAKNKLWSPFGYPVHHANEASAQVGSIVNCMFNRDCMTHTHINFIGSGLPL
                     KLQREVAKELFGSEDAYDETKNYTPINDAKIKYAKWSLLRVCLHNAVTLCNWVWPMTV
                     SPLKSRNYRGDLALEAKFFKAITGEDMTQEKLDLAAERIFTLHRAYTVKLMQTKDMRN
                     EHDLICSWVFDKDPQIPVFTEGTDKMDRDDMHASLTMFYKEMGWDPQLGCPTRETLQR
                     LGLEDIAADLAAHNLLPA"
     gene            complement(16339..16965)
                     /locus_tag="NBI74_003208"
     CDS             complement(16339..16965)
                     /locus_tag="NBI74_003208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein"
                     /protein_id="HCD1933492.1"
                     /translation="MNPVDRPLLNIGLTRLEFLRISGKGLAGLTIAPALLSLLGCKQE
                     DIDSGTVGLINTPKGVLVTQRARCTGCHRCEISCTNFNDGSVGTFFSRIKIHRNYFFG
                     DNGVGSGGGLYGDLNYTADTCRQCKESQCMNVCPIGAITWQQKEGCITVDHKRCIGCS
                     ACTTACPWMMATVNTESKKSSKCVLCGECANACPTGALKIIEWKDITV"
BASE COUNT         4169 a         4437 c         4015 g         4506 t
ORIGIN      
        1 gttaccagtg aaaatttaag gtcggtgctc atcaagtttt ctcctttttt attaccacac
       61 aaaaagtgat attacgcatt tttacacact gtgatgaaaa aatctcccgt catttataat
      121 gataagtgtt tttactactt ccccttttcg tcaagatcgg ccaaaattcc acgcttacac
      181 tatttgcgta ctggccattg accccttcct gacgctccgt gtcgtttttc cggcgtaccg
      241 caacattttt gttgtgcgta aggtgtgtaa aggcaaacgt ttaccttgcg aatttgcagg
      301 agctgaagtt agggtctgga gtgaaatgga atggcaacaa taaaagatgt agcgaaacga
      361 gcaaacgttt ccactacaac tgtgtcacac gtgatcaaca aaacacgttt cgtcgctgaa
      421 gaaacgcgca atgccgtgtg ggcagcaatc aaagaattac actactcccc tagcgcggtg
      481 gcgcgtagcc tgaaggttaa ccacaccaag tctatcggtt tgctggcgac cagcagcgaa
      541 gcggcctatt ttgccgagat cattgaagcg gtcgaaaaaa attgcttcca gaaaggttac
      601 accctgattc tgggcaatgc gtggaacaat cttgagaaac agcgggctta tctgtcgatg
      661 atggcgcaaa aacgcgtcga tggtctgctg gtgatgtgtt ctgagtaccc agagccgttg
      721 ctggcgatgc tggaagagta tcgccatatc ccaatggtgg tgatggactg gggtgaagca
      781 aaagctgact tcaccgatgc ggtcattgat aacgcgttcg aaggcggcta catggccggg
      841 cgttatctga ttgaacgcgg tcatcgcgaa atcggcgtta tccctggccc gctggaacgt
      901 aacaccggcg caggccgtct tgctggtttt atgaaggcga tggaagaagc gatgatcaaa
      961 gtgccggaaa gctggattgt gcagggtgac tttgaacctg aatccggtta tcgcgccatg
     1021 cagcaaatcc tgtcgcagtc gcatcgccct actgccgtct tctgtggtgg cgatatcatg
     1081 gcaatgggcg cactttgtgc tgctgatgaa atgggtctgc gcgtgccgca ggatgtttcg
     1141 ctgatcggtt atgataacgt gcgcaacgcc cgctatttta cgccggcgct gaccacgatc
     1201 catcagccaa aagattcgct gggcgaaaca gcgttcaaca tgctgttgga tcgtatcgtc
     1261 aacaaacgtg aagaaccgca gtccattgaa gtgcatccgc ggttgattga acgccgctcc
     1321 gtggctgacg gcccgttccg cgactatcgt cgttaatcac ccgttgcggg agtctcttcc
     1381 ggctcccgca gccactcctt attcagcgtc tcactatcgc cgagatactc aagcaaccag
     1441 gttaacgcag gcgacatatc attttgctgc catgtcagac aacatgccga atccgggaag
     1501 gggttttcca gttttagtgc tacccacttc ccctcattaa gccacggttt ggcgaaatgt
     1561 gttggcacca tccctatgca taatcctgcc gagatacagg ttgccgatga ttcccagtca
     1621 ggcacgacaa gtcttttttg gttatccagc aaccaggtaa tacgtttagg tagcgttcgc
     1681 gaggtgtctt cgcgcaccaa tgacggccag ttgcgcaacg tatcatcgct gaacgggcca
     1741 tccatcaacg ccagcgggtg gtggctggca acaacgcaac tccagcttaa catccccata
     1801 tcccggaagg cataacgacc gcctaccgga atcgcgcgtg ttgcgccaat cgccagttcc
     1861 acgcgcccgt cggaaagtgc atcccagaca ccgttgaaca cttcctgaaa gacaaggagt
     1921 tcgacatcat caaaatggcg ataaaaatca acgatcatct gccgtgttct ctctggcctg
     1981 acaatattat ccactgcgat agctaactga ccgcgccagc cgttcgctat ctgctgacat
     2041 tgctggcggg tgatctgcat ttttttgaca acagagcgcc cttctttgag aaaccacgcc
     2101 ccggctgcgg tcagttccac atcacggtga cgtcgttcaa acagcggcac cgccagccac
     2161 tcttccagct gacgcacggt atagctgacc gcagaaggaa cgcgatgcaa ctcctgggct
     2221 gcggcgctaa aactaccatt acgcgctacc gcatcaacaa cttcgagaga atattctgac
     2281 cacatagtct gcctgcaaaa tttttgaaac caatcatcaa atattaccgt ttcacaacac
     2341 taatttcact ccctacactt tgcggcggtg tttaattgag agatttagag aatatacatg
     2401 caacctggga aaagattttt agtctggctg gcgggtttga gcgtactcgg ttttctggca
     2461 accgatatgt atctgcctgc tttcgccgcc atacaggccg acctgcaaac gcctgcgtct
     2521 gctgtcagtg ccagccttag tctgttcctt gccggttttg ccgcagccca gcttctgtgg
     2581 gggccactct ccgaccgtta tggtcgcaaa ccggtgttat tcatcggcct gacaattttt
     2641 gcgttaggta gtctggggat gctgtgggtg gaaaacgccg ccacgctgct gatattgcgt
     2701 tttgtacagg ctgtgggtgt ctgcgccgcg gcggttatct ggcaagcatt agtgacagat
     2761 tattatcctt cacagaaagt taaccgtatt tttgcgacca tcatgccgct ggtgggtcta
     2821 tctccggcac tggctcctct gttaggaagc tggctgctgg tccatttttc ctggcaggcg
     2881 attttcgcca ccctgtttgc cattaccgtg gtgctgattc tgcctatttt ctggctcaaa
     2941 cccacgacga aggcccgtaa caatagtcag gatggtctga cctttaccga cctgctacgt
     3001 tctaaaacct atcgcggcaa cgtgctgata tacgcagcct gttcagccag tttttttgca
     3061 tggctgaccg gttcaccgtt catccttagt gaaatgggtt acagcccggc agttattggt
     3121 ttaagttatg tcccgcaaac tatcgcgttt ctgattggtg gttatggctg tcgcgccgcg
     3181 ctgcagaaat ggcaaggcaa gcagttatta ccgtggttgc tggtactgtt tgctgtcagc
     3241 gtcattgcga cctgggctgc gggcttcatt agccatgtgt cgttggtcga aatcctgatc
     3301 ccattctgtg tgatggcgat tgccaacggc gcgatctacc cgattgttgt tgcccaggcg
     3361 ctgcgtccct tcccacatgc aaccggtcgc gccgcagcgt tgcagaacac tctgcaactg
     3421 ggtctgtgct tcctcgcaag tctggtagtt tcctggctta tcagtatcag tacgccattg
     3481 ctcaccacca ccagcgtgat gttatcaaca gtagtgctgg tcgctctggg ttacatgatg
     3541 caacgttgtg aagaagttgg ctgccagaat catggcaatg ccgaagtcgc tcatagtgaa
     3601 tcacactgat ctatatcgat atacttatac ttaggctgct aacaaaattt tgttgtatga
     3661 ttgaaattag cggcctatac taatttcgag ttgttaaaac tacgataaat attatgttat
     3721 tacggggaca ggatcgttcc cgactcacta tggatggtca tttcggcaag ggttcctcct
     3781 ttccctctgt tctacgtcgg attatagact cgcggttttt tctgcgagat ttctcacaaa
     3841 gcccaaaaag cgtctacgct gttttaaggt tctgatcacc gaccagtgat ggagaaacta
     3901 tgagttcatc gtgtatagaa gaagtcagtg taccagatga caactggtac cgtatcgcca
     3961 acgaattact tagccgtgcc ggtatagcca ttaacggttc tgccccggcg gatattcgtg
     4021 tcaaaaaccc cgattttttt aaacgcgttc tgcaggaagg atcgttgggg ttaggcgaaa
     4081 gttatatgga tggctggtgg gaatgtgacc gactggatat gttttttagc aaagtcttac
     4141 gcgcaggtct cgagaaccaa cttccccatc atttcaaaga cacgctgcgt attgccggcg
     4201 ctcgtctctt caatctgcag agtaaaaaac gtgcctggat agtcggcaaa gagcattacg
     4261 atttgggtaa tgacttgttc agccgcatgc ttgacccctt catgcaatat tcctgtgctt
     4321 actggaaaga tgccgataat ctggaatctg cccagcaggc gaagctcaaa atgatttgtg
     4381 aaaaattgca gttaaaacca gggatgcgcg tactggatat tggctgtggc tggggcggac
     4441 tggcgcacta catggcgtct aattatgacg taagcgtggt cggcgtcacc atttctgccg
     4501 aacagcaaaa aatggctcag gaacgctgtg aaggcctgga tgtcaccatt ttgctgcaag
     4561 attatcgtga cctgaacgac cagtttgatc gtattgtttc tgtggggatg ttcgaacacg
     4621 tcggaccgaa aaattacgat acctattttg cggtggtgga tcgtaatttg aaaccggaag
     4681 gtatattcct gctccatacc atcggttcga aaaaaaccga tctgaatgtt gatccctgga
     4741 ttaataaata tatttttccg aacggttgcc tgccctctgt acgccagata gctcagtcca
     4801 gcgaacccca ctttgtgatg gaagactggc ataacttcgg tgctgattac gatactacgt
     4861 tgatggcgtg gtatgaacga ttcctcgccg catggccaga aattgcggat aactacagtg
     4921 aacgctttaa acggatgttt acctattatc tgaatgcctg tgcaggtgct ttccgcgccc
     4981 gtgatattca gctctggcag gtcgtgttct cacgcggtgt tgaaaacggc cttcgagtcg
     5041 ctcgctaaag gctattctat cgcccccgct ccgggggcga tttcagatca ggcttctgta
     5101 cctggttgat tcatggcatt ttctcgtgcc gccagcacac gttctaccgt atctaccact
     5161 gcctgagttt gtggatcgat ttcaatgttg acgcgtgcgc cgagtttttt cttcccaaga
     5221 gtcgtgcgtt ccagtgtttc cggaattaaa tggacgcaaa aacgcgttgg cgtgacttcg
     5281 ccgacggtca ggctaatacc gtcgatgcca ataaatcctt tgtacaggat atatttcatc
     5341 aactgactat cctggacttt aaaccagatc tgacgattat tttccgaggt taatattttc
     5401 gccacttcag cggtggtcat aatatgacct gacattaagt gtccgccaat ttcatcgctg
     5461 aatttcgccg cacgctcaac gtttacccaa tcccccactt ttaaatcacc aagattagta
     5521 atgcgtaacg tttctttcat caggtcaaaa ctgacatggt tgccgttaat ttccgtcacg
     5581 gtcaggcagc aaccgttatg cgccacggac gcaccggttt ccagtccgtc cagcatgtgg
     5641 tcgggtaact ccaccacatg cgtgcgaaaa tttggtttct cgtcaatcga caccagtttt
     5701 gcggtgccct gtacaatccc cgtaaacata cttacaactc ctgaaatcag ttaagacatt
     5761 ctgttcagca caatagcagg tggaaaacgc ccttaccagt gaaggggtaa gaatggctat
     5821 tttttcactg gagaattaat aaatcctcgc tacaatagac tgaatttccc ctgcttcttc
     5881 tttttgctgc ccattcaggc ggctttttag tctctcatat aactacaaat aaaaggtgtt
     5941 cacgtgcaga agtatatcag tgaagcgcgt ctgttattag cattagcaat cccggtgatt
     6001 ctcgcgcaaa tcgcccaaac tgcgatgggt tttgtcgata ccgtgatggc gggcggctat
     6061 agtgccaccg acatggcggc ggttgctatc ggaacttcta tctggcttcc ggcgatcctc
     6121 tttggtcacg gactgctgct ggcattaacg ccggttatcg cgcaattaaa tggttccggt
     6181 cgacgtgagc gcattgcgca tcaggtgcga caaggtttct ggctggcagg ttttgtttcc
     6241 gttctcatta tgctggtact gtggaatgcg ggttacatta tccgctccat ggaaaacatc
     6301 gatccggctc tggcggacaa agccgtgggt tatctgcgtg cgttgctatg gggcgcgccg
     6361 ggctatcttt tcttccaggt tgcccgtaac cagtgtgaag gtctggcaaa aaccaagccg
     6421 ggtatggtga tgggctttat cggcctgctg gtgaacattc cggtgaacta tatctttatt
     6481 tatggtcatt tcggtatgcc tgagctcggt ggcgttggtt gtggcgtggc tactgcggcg
     6541 gtgtattggg tcatgttcct tgccatggtt tcttacatta aacgcgcccg ctcgatgcgc
     6601 gatattcgta acgaaaaagg caccgcgaag cccgatcctg cggttatgaa acgactgatt
     6661 caactcggtt tgccaattgc gctggcactg ttctttgaag taacgctgtt tgccgtcgtg
     6721 gctctgttag tgtctccgct cggtattgtt gatgtcgcag gacatcagat tgccctgaac
     6781 tttagttcac tgatgttcgt gcttcccatg tcgctggcgg cagcggtaac catccgcgta
     6841 ggttatcgtc tgggtcaagg ctcaacgctg gatgcgcaaa ccgctgcgcg gaccgggctt
     6901 atggtgggtg tctgtatggc aaccctgacg gccatattca cggtttcact gcgagagcaa
     6961 atcgccctgt tgtacaacga caatcccgag gttgtaacgc tggctgcgca tttgatgctg
     7021 ctggcggcgg tatatcagat ttctgactct atccaggtga ttggcagtgg gattttgcgt
     7081 ggttataaag atacacgttc cattttctat atcaccttta cggcttactg ggtgctgggc
     7141 ttgccaagcg gctatattct ggcactgacc gatctggtcg ttgaacctat ggggccagca
     7201 ggcttctgga taggctttat tattggcctg acttcggcag ccattatgat gatgctgcgt
     7261 atgcggttcc tgcaacgtct gccgtcagcc atcattctgc aacgagcagc tcgctagtaa
     7321 agacaaggcg caaccttcac gggttgcgcc tgtgttttta cgcaggctgg agcgttgcgc
     7381 caatcccgtc ttcgtctggc tgtagtttca gtgcgttaca cagagtttgc cgactggcat
     7441 ctgttatctc aacgtgtata tcaccagtaa cttccccttt cacattgatg aagtcaggtg
     7501 aaatttcttt cgcactggtt tgcaggcaaa tggctccctc tccgcgcagg cgtttcactt
     7561 tcagttgcac atgcgttgcc gcatcagtga tcagccacag ttttccgcca tttagattga
     7621 tgttaccaat accaaaagtg acactgtcac cttttaccgt actgccgtcg agaatcaata
     7681 caccgccctg ctgtacagta atcgttgatt gttgcagatc tgcgccatag ccaataatca
     7741 ttgaagcgcc gtccagtaat gcaacactac cggtcagttc aaccggtgcg cgatcttcct
     7801 ctacgacaaa attttcgtcc gcgctggtgg gtgacaacag gatcactgtc ccaccttgta
     7861 ttaccgcatt ttccagcgta ccggcatgca cttcagcccg accacccaca ttaacggaca
     7921 ggttttgcgt cttaccggaa ctgtagagct gaagaccatc cgtccccaga cgatagatgg
     7981 ctccattctc aacaatagtg tcagagtctt caccaccgag attattcatg gtgccaccag
     8041 caagcaacgt atcactggct ttccccgcca gcataaaaac gccgccctga ttaatctcac
     8101 aattgcaggc ttccccacct ggatacacca acaattcacc accttcatct accacgaccg
     8161 ctgaggttgt cccattgacc aacaggccac cctcttgatc gagaataatt ttttccgcgc
     8221 gatggccttc cagtacacgc aggttaccgc cattttccag caacaaaccg caggcataac
     8281 ctttatcaac gctgaattca ccttcgggat ggcggccatt aaccgtagcg agcgtagaga
     8341 gactaattgc gcctccctcg gactgaacaa tatctgtggc aatggcaccg tcgctgaccg
     8401 atagtgtgcc attgttaccg tactgtgtgc atttggctat cccaccggtt gccactttca
     8461 accagccagc ccccgtgacc acggtatttt cggctcttcc gccagcttca acaatcaact
     8521 cgccttcagt aagttgcgtc gcgccaataa cctcgccggt cttaacaatt tgcacgttcc
     8581 cgtcgctcat caagtttttc gcatcagatc ccatgattta ttcctttgct gcatctgtgt
     8641 gcctttattg ctacctaagt gtaaaggcta cgaaggattt atccacgaca gatttgagat
     8701 ggtggcaaac aactctgttt aaactctgat acacggatta ttgggttgta tcagatgtaa
     8761 atgcgatcct gaataaaaat cgcccttgca aatcaacaaa atatagccaa tttgcttaaa
     8821 agctctgcaa acggtcgaaa tagcgcagaa aattacgttt tgcctcttgc caccttccca
     8881 ctctgccgct aatattcgtc cccgttgtca cctacaacgt tgcgttcata gctcagttgg
     8941 ttagagcacc accttgacat ggtgggggtc gttggttcga gtccaattga acgcaccatc
     9001 ctgcgtccgt agctcagttg gttagagcac caccttgaca tggtgggggt cggtggttcg
     9061 agtccactcg gacgcaccag attttcttaa tctggtcttc tccttttttc cctctgtttc
     9121 ttctctgtat ccaatacgtt aaaagattta cactgtcttc gtatgcgtta tcagaaggag
     9181 aatcgctatg gcaactttgt tacaacttca tttttctttc aatggcccat ttggcgacac
     9241 aatggctgag cagcttaaac cgcttgctga gtcgattaat caggaacctg gttttctgtg
     9301 gaaggtttgg acagaaagtg aaaagaacca cgaagccggt gggatctacc ttttcactga
     9361 tgaaaaaagc gcccttgcct atctggaaaa acatactgcc cgactgaaaa atctcggcgt
     9421 tgaggaagtt gtcgccaaag ttttcgatgt caatgtgcca ctgagtcaaa tcaatcaggc
     9481 aaaacttgcc tgacagattt taatcaaggg cggttagtgc ccttttcatc cctgtctgaa
     9541 atttctcaaa ttctaaaaat ctcaaccaaa cttacctgat aacactaaat tcgaaagaat
     9601 gcgtacaggt aagtaacaat gaaaacaatt gctattgtgg gtgccgggcc tacggggatc
     9661 tacaccttat tctcgcttct acagcaacaa acgccacttt ctatttctat cttcgatcag
     9721 gctgacgaag ccggtatcgg gatgccatac agtgatgagg aaaactcaaa actgatgctg
     9781 gcaaacattg ccagtattga aataccgccg atttattgca cgtatctcga atggctacaa
     9841 aagcaagaag ccagccatct ccagcgttat ggcgttaaaa aagaaacctt gcacgatcgt
     9901 cagtttttac cgcgaattct gctgggcgaa tatttccgcg atcaattttt acgattagta
     9961 gaccaggcac gacagcaaaa atttgcagtg gctgtttatg aatcatgcca ggttaccgat
    10021 ctgcaaatta caaatgctgg cgtcatgctc gctacaaatc tgaatttacc cagcgagacg
    10081 tttgatttag cggtgatcgc aacggggcac gtctggcctg atgaagaaga agcaaccaga
    10141 acgtattttc ccagcccgtg gtcaggcctg atggaagcaa aggtcgatgc gtgtaacgtg
    10201 ggtattatgg gaacatcctt gagcggactg gatgcggcaa tggcagtggc tattcagcat
    10261 ggttcgttca ttgaagatga taaacaacac gtcacttttc accgcgataa cgcaaatgaa
    10321 aagctaaata ttacgttaat gtcgcgcaca ggtattttac ccgaagccga tttctattgc
    10381 cctattccct acgagccctt acacatcatc accgatcagg cattaaatgc tgagattcaa
    10441 aaaggcgaag tgggcctttt ggatcgggta tttagattga tagtagagga aatcaagttt
    10501 gctgatccag actggagcca acgcatagcc ttagagagcc tgaatgtcga ttcctttgct
    10561 caagcctggt ttgccgagcg caaacaacgc gacccatttg actgggcaga agaaaatctc
    10621 caggaagtcg aacgcaataa acgagaaaaa catactgttc cctggcgtta tgtcattctg
    10681 cgcctgcatg aagccgtaca ggaaattgtt ccacatctga atgaacacga ccataaacgg
    10741 ttcagtaaag gccttgcccg ggttttcatc gataattatg cggcaatccc ttcagagtct
    10801 attcgtcgac tactggcctt acgtgaagcg ggaatcattc atattctcgc cctcggtgaa
    10861 gactacgaaa tggaaattaa tgagtcgcgc accgtcctga aaacggaaga caatagctac
    10921 tcgtttgacg tttttattga tgcccgcggg cagcgtccgc ttaaagtgaa agatattcct
    10981 ttccctggac tacgcgaaca attacagaaa acaggggatg aaatccctaa tgttggcgaa
    11041 gattatacgt tacagcaacc cgaagatatt cgtgggcgcg tagcgttcgg cgcgttgccc
    11101 tggttgatgc acgaccagcc tttcgttcag ggaattacgg catgtgcaga aattggagag
    11161 gcgatggctc gggcggtcgt aaaacctgca tcccgtgcgc gtcggcgtct ttcgtttgat
    11221 taaaggtaaa gctatcagca ctcaacaaac tgatgttgtt cattaagacg atagcggact
    11281 ccggctcgaa tattgttttc accttcaatg gcgacggcga agcgaactct ttcgccgtca
    11341 tgatatgcca gtgcggcgca tccccctggc ccgagaataa ttgagtcgac cacgcccgtg
    11401 ctcactaccg ttgaattttc accactggca atgatgtgta cattgtcgcc gctgttggca
    11461 attctggcgt ttgcgccaaa gctggcgatt tgcaccaggt caccattact ggcgacatga
    11521 caccgttcac ccaatgtaca aacgcgtact cgcattccgg tattcgcaat ccgaccgcta
    11581 tctccagcgc tgcttatccg cgaagaatta ccggttacag caacccgtgc gcgtgcaccg
    11641 gagctgccaa tgtgactgtt aaagccgaca cttccaatcc gcacgctata acccatactg
    11701 gcaatttgcg cggcataccc agcggttgcg attctggcgt tatcctcaca acagccgagg
    11761 cgagcatgat cgtcgctgcg gacaaactga ctgcaaaata gtcgttctcc gggatctgtg
    11821 gccagttttt gcatagagga aacttcctga tgcgcgaagt tttcatctgc cagccatttt
    11881 ttccacgcat actcaaccag actctccgcc cagtccgtat atccttcctc aatcagtgca
    11941 tgatgaatat cggcataact accgccacgt gggaaatggc gcagaaacca gcgatacatc
    12001 accgctccga tacgccattc tcgcaggtct gctcgtgtaa tgatcattcg ttagtgccgg
    12061 tgatagccat cgaccatgct tttaaaggtt tcgtgtggcg tacgccccgt ggtgcaaaga
    12121 taaagatgac cgaagataaa aaagaggctt ataaatgcca gagcaaaatg cgcctgcaat
    12181 aaccagtatc ttacgccagg aaacacatct cccacggctt gcggatagag acacagcagc
    12241 cccgtcaata gtaacaatgg caacaatcca tacatgacac caacataggc gacctgctgt
    12301 aagggattaa atttagactg ggttgttgcc gggaaaggat gttcttcccc ctgcataatg
    12361 ccaaacaaat aaaatcgcgt ttgttttgcc gctcgatcca gccaaccctg acgacgaatg
    12421 cgatagtggt gaccattacc cccaacggca ttgatcagaa caaagccaag ccagcatgcc
    12481 agtaacaaaa atccgcaaac ttcatgcacc gcaaccagac ttttaaccgc agttgcgccc
    12541 accagcgcaa agtgatttat cagcccactt gccagcaaca atacaaagag taacgcattc
    12601 gaccagtgcc ataatctgac cgctttgctg taaagataaa tcctctcact atgatcggtg
    12661 gctgatttct tcacgccacg agcacgaagc aaggcatgta gccccagcac caaccacatc
    12721 ccaaccagca atagtccggc aatgatcaac cacaccggcc aaaattgggg ggtgaactgt
    12781 ggcacatagt tcgccaactg gctctgaaac tgttcagcat gttgcgacgg gttcatacat
    12841 tttccttttt aatcaaatgt tgaccgaacc ggcgatacag atgcggtttc ccggcgccag
    12901 gtagctgata ttgatagtat ttattttcct gcaaccacgc ctgaatttct gggctatctt
    12961 cgtgaccaaa gatcaatgcg tgttccgggc aggcactgac gcaaatgggc ggaaagcctt
    13021 ttgccagtcg ggtctcagcg cagaaatcac atttatctgc cactttagtg acagggttaa
    13081 ggtagcgaac ctggtacggg caaacgccaa tgcaataact gcagccgatg cactgcgatt
    13141 tttctacccg cacgatcccc tgctcatcac gccacgacgc ccctgtcggg caaacgtcga
    13201 tgcaaggagc atcttcgcag tgctgacatg actgacgaaa aaagtgatac tgcgttccat
    13261 tgtcgttatc gatgactgga atgtgtgcta tcgataaacg gcttccctga gcggggacgt
    13321 ggttagtttt acggcatgcg cgggcgcaga tattacagcc attgcaccgt gactcgtcat
    13381 gaatcatggc ataccgaact tccttttctt gcctcgtatt cgctaacaga gaagacgcag
    13441 aaccagtaaa aaagattact gttcccatcc ccagaacaaa tttgcgtcga gtgaaggaca
    13501 tctgattatc ccctaatgca gtggctgcaa ctgtccaccc tatccatgac atccccctga
    13561 aatttcaggg gttctgaaac tgctctacct cgaaccattc gcataagcca tacgccttgc
    13621 cataggtcat aaatcgctcg ctaaacaagt acaacactgc cgtcgatgac tccacctgcc
    13681 gtatatcggc atattcaggt gcaacatcca tcgcggcgat cgcggcttca atctgcgctt
    13741 cctggaagta atatggcgct tgcatcagca tggcgacttt gtaaggacga ggatacgtct
    13801 ggcactcaaa acgaacggcg tgtgcaatgg cgcgacaaat gtcctgctcc acaacttgca
    13861 aagacatcgc cgcgtagttt tcactcatgg cctgcgttga gtaatagtaa tcatcctggc
    13921 ttcccgtcat acaagcgata tccgcgtagt ctccgttctg cttaatgttt tccagcaaaa
    13981 ctgacagttc ctcttctgca accgaatagg gtggcgagag aaacacggcg cgcgccacca
    14041 gctgcccagc cgcagaatgc tggcgaatga actctgccag gatttcagcc ggttctggtt
    14101 cggttaccgt atcaacgggg tgacgcatcc cctgcaacca ttgccgcttc gcctcatcaa
    14161 tttcgctgac tttcgctaac ggcaattcat cctgatggtt catctctccc attccttacg
    14221 caggcagtag attgtgtgct gccagatcgg cggcaatatc ttccagcccc agacgctgca
    14281 atgtttcgcg ggtcggacaa ccaagctgtg ggtcccagcc catttctttg tagaacatcg
    14341 tgagcgaagc atgcatatcg tcacggtcca ttttgtctgt accttcagta aagaccggga
    14401 tctgcggatc cttgtcgaat acccaggaac agataagatc gtgttcatta cgcatatctt
    14461 tggtttgcat cagttttacc gtgtaggcgc gatgcaacgt aaagatacgc tctgcagcta
    14521 aatctaattt ttcctgagtc atatcttcgc cagtgatcgc tttgaagaat ttggcttcaa
    14581 gcgccagatc accccggtaa ttacggcttt tcagcggcga aacggtcatt ggccagaccc
    14641 agttgcacag ggtgacggcg ttatgcaaac agacccgcaa cagcgaccac ttagcatatt
    14701 taatttttgc gtcattgatc ggtgtgtagt ttttggtttc atcgtaagca tcttcagaac
    14761 caaaaagctc tttcgccact tcacgctgca gtttcaatgg caagccggaa ccaataaaat
    14821 tgatatgggt atgcgtcatg cagtcacggt tgaacataca gttaacaatg gaaccgactt
    14881 gcgctgacgc ttcattggca tggtgaaccg gatagccaaa cggcgaccag agtttatttt
    14941 tcgcgtagcc ccagtactct tcacccaaat tccagcgttc tgcgatggca tatgaaccat
    15001 cagccaggtg actcaactca cccacgcgat gcgccagacg gtagtaaaaa tctttaataa
    15061 agttaacgtc acccgcttcc agttgatccc agcgaatttc cgcatactct tcagcaggca
    15121 gaacacgctt gaacacacct ttgctgtaac aataagtaaa gtcgcgatgc aactgcccgt
    15181 agttacacca taggccgtag tcatcgaaca aattcagacc aaccaggtta ccaatcacac
    15241 gaccatcgtc cttatcttca aaatctttcg gcccgttcgg gaagatggtg gtatggacaa
    15301 agtttgctac acaagtgtta ccacctgtgc tgggcacgcc aaactctttc acccgaggaa
    15361 tattcatttg ggtcatacaa cggatcgggc aagagtggca gccactcatt tttactgtgt
    15421 atttctctgc cgccggacct aagtcaaaaa cggatttata ggtacgaaag ccgaccgtat
    15481 tctgattgcc tggcggaatt tcccccgttt caatcggacc accttcagcc gcgccccaaa
    15541 acagcccttt acgtgctgtc caacgtgact tgggatctga atactctgcc cacgattgcg
    15601 gcgtacttgg cacgacatgg ttgttattcg caccgataag ttcagtcatc atgtaatcgt
    15661 tgagacgctt catctcctga cgatcggcaa tgttgacccc tttcgtccct tcaaccgcaa
    15721 tcgctttcag gtttttcgaa cccattattg cgccagttcc cgcaccgccg ctgtggttac
    15781 ggctattcaa catgccagag agaggaacaa ggttttctcc agcctgacca atagccgcca
    15841 cacaggtttc cggactggtc aagcgacaaa tttcttccgt cgttgcgcgc gtcccttttc
    15901 cccataagaa atcggctttt tcaaggctaa ctttgtcatc tttaatcttc agccataccg
    15961 gtgatttcgc tttcccttcg ataataatga cgtcgtatcc agcgaatttc atttgcgctg
    16021 caaaaaagcc ccccatatgg gcatcgacga ctaaatttcc tttggtaaaa gtagaaagtg
    16081 aggtgatatt tacgcgagaa ctacaggggg caccagatcc agttaatggg ccggtagcaa
    16141 aaactaattt attcgcttca tcgaaaggtt tcgtgcctgg cggtacttcg tcatacataa
    16201 ttttgtagcc gaagcccatg ccaccgacaa aacttttaaa tttactggaa tcttcgaggg
    16261 taatatttcc tgtcgtgaga ttgactctta atatattacc tgtccaaccg ttagccatga
    16321 ttttttcctt tgcaagattc acacagtaat atctttccac tcgataattt ttaacgcccc
    16381 tgttgggcag gcgtttgcgc attcaccgca taacacacat ttcgaggatt ttttactttc
    16441 ggtatttacg gtggccatca tccacgggca tgccgtagta caggcgctac agccaataca
    16501 acgtttatga tcgacggtaa tacagccttc tttctgctgc caggtaatcg caccaatcgg
    16561 gcagacgttc atgcattgcg actctttgca ttgacggcag gtatccgcgg tatagttgag
    16621 atcgccatac aggccaccgc cagagccaac cccgttgtcg ccaaagaaat aattgcgatg
    16681 gattttgata cgggagaaga atgtccctac tgagccatcg ttgaagttgg tacaagagat
    16741 ttcacagcgg tggcaacccg tgcatcgcgc acgttgggtc accagcaccc ctttgggtgt
    16801 atttatcaac cctactgtgc cgctatcaat atcttcctgt ttgcaaccca aaagtgacag
    16861 taaagcaggg gcaattgtta aaccagcaag gccttttcct gatatacgca agaattctaa
    16921 tcgcgttaag ccaatattta atagtggacg atcaaccggg ttcatttatt tcgcatcctc
    16981 ttcgcaaaca cagtgctgac aattcaacag cctgatatta agcactcaac taatacgctg
    17041 ctgacgctgt cctcaacacc agatattttc cctaacccga tagtgtatga gaaaagattt
    17101 aacgctaaca aactaatcct ttatggt
//
LOCUS       DAHHTG010000095        16919 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.095, whole genome shotgun sequence.
ACCESSION   DAHHTG010000095 DAHHTG010000000
VERSION     DAHHTG010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16919)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16919)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16919
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.095"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            595..729
                     /locus_tag="NBI74_003209"
     CDS             595..729
                     /locus_tag="NBI74_003209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001186445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Hok family toxin"
                     /protein_id="HCD1933493.1"
                     /translation="MIVALIVICITAVVAALVTRKDLCEVHIRTGQTEVAVFTAYESE
                     "
     gene            complement(833..1963)
                     /gene="dnaJ"
                     /locus_tag="NBI74_003210"
     CDS             complement(833..1963)
                     /gene="dnaJ"
                     /locus_tag="NBI74_003210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HCD1933494.1"
                     /translation="MAKQDYYEILGVSKTAEEREIKKAYKRLAMKYHPDRNQGDKEAE
                     AKFKEIKEAYEVLTDSQKRAAYDQYGHAAFEQGGMGGGGFGGGADFSDIFGDVFGDIF
                     GGGRGRQRAARGADLRYNMELTLEEAVRGVTKEIRIPTLEECDVCHGSGAKPGTQPQT
                     CPTCHGSGQVQMRQGFFAVQQTCPHCQGRGTLIKDPCNKCHGHGRVERSKTLSVKIPA
                     GVDTGDRIRLAGEGEAGEHGAPAGDLYVQVQVKQHPIFEREGNNLYCEVPINFAMAAL
                     GGEIEVPTLDGRVKLKVPGETQTGKLFRMRGKGVKSVRGGAQGDLLCRVVVETPVGLN
                     EKQKQLLQELQESFGGPTGEHNSPRSKSFFDGVKKFFDDLTR"
     gene            complement(2052..3968)
                     /gene="dnaK"
                     /locus_tag="NBI74_003211"
     CDS             complement(2052..3968)
                     /gene="dnaK"
                     /locus_tag="NBI74_003211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001737966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HCD1933495.1"
                     /translation="MGKIIGIDLGTTNSCVAIMDGTTPRVLENAEGDRTTPSIIAYTQ
                     DGETLVGQPAKRQAVTNPQNTLFAIKRLIGRRFQDEEVQRDVSIMPFKIIAADNGDAW
                     VEVKGQKMAPPQISAEVLKKMKKTAEDYLGEPVTEAVITVPAYFNDAQRQATKDAGRI
                     AGLEVKRIINEPTAAALAYGLDKGTGNRTIAVYDLGGGTFDISIIEIDEVDGEKTFEV
                     LATNGDTHLGGEDFDSRLINYLVEEFKKDQGIDLRNDPLAMQRLKEAAEKAKIELSSA
                     QQTDVNLPYITADATGPKHMNIKVTRAKLESLVEDLVNRSIEPLKVALQDAGLSVSDI
                     DDVILVGGQTRMPMVQKKVAEFFGKEPRKDVNPDEAVAIGAAVQGGVLTGDVKDVLLL
                     DVTPLSLGIETMGGVMTTLIAKNTTIPTKHSQVFSTAEDNQSAVTIHVLQGERKRAAD
                     NKSLGQFNLDGINPAPRGMPQIEVTFDIDADGILHVSAKDKNSGKEQKITIKASSGLN
                     EDEIQKMVRDAEANAEADRKFEELVQTRNQGDHLLHSTRKQVEEAGDKLPADDKTAIE
                     SALTALETALKGEDKAAIEAKMQELAQVSQKLMEIAQQQHAQQQTAGADASANNAKDD
                     DVVDAEFEEVKDKK"
     gene            4339..4743
                     /locus_tag="NBI74_003212"
     CDS             4339..4743
                     /locus_tag="NBI74_003212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2541 family protein"
                     /protein_id="HCD1933496.1"
                     /translation="MKSVITISASLAISLMLCCTAQANDHKILGVIAMPRNETNDLAL
                     KLPVCRIVKRIQLSADHGDLQLSGASIYFKATRSASQTLNIPSEIKEGQTTDWININS
                     DNDNKRCVSKITFSGHTVNSSDMATLKIIGDD"
     gene            4769..5482
                     /gene="msyB"
                     /locus_tag="NBI74_003213"
     CDS             4769..5482
                     /gene="msyB"
                     /locus_tag="NBI74_003213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acidic protein MsyB"
                     /protein_id="HCD1933497.1"
                     /translation="MNVNYLNDSDLDFLQHCSEEQLANFARLLTHNEKGKTRLSSVLM
                     RNELFKSMEGHPERHRRNWQLIAGELQHFGGDSIANKLRGHGKLYRAILLDVSKRLKL
                     KADKEMSTFEIEQQLLEQFLRNTWKKMDEEHKQEFLHAVDARVNELEELLPLLMKDKL
                     LAKGVSHLLSSQLTRILRTHAAMSVLGHGLLRGAGLGGPVGAALNGVKAVSGSAYRVT
                     IPAVLQIACLRRMVSATQV"
     gene            5631..6197
                     /gene="satP"
                     /locus_tag="NBI74_003214"
     CDS             5631..6197
                     /gene="satP"
                     /locus_tag="NBI74_003214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate uptake transporter"
                     /protein_id="HCD1933498.1"
                     /translation="MGNTKLANPAPLGLMGFGMTTILLNLHNVGYFALDGIILAMGIF
                     YGGIAQIFAGLLEYKKGNTFGLTAFTSYGSFWLTLVAILLMPKLGLTDAPNAQFLGVY
                     LGLWGIFTLFMFFGTLKGARVLQFVFFSLTVLFALLAIGNIAGNAAIIHFAGWIGLIC
                     GASAIYLAMGEVLNEQFGRTVLPIGESH"
     gene            complement(6232..6819)
                     /gene="mog"
                     /locus_tag="NBI74_003215"
     CDS             complement(6232..6819)
                     /gene="mog"
                     /locus_tag="NBI74_003215"
                     /EC_number="2.7.7.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin adenylyltransferase"
                     /protein_id="HCD1933499.1"
                     /translation="MNTLRIGLVSISDRASSGVYQDKGIPALEEWLTSALTTPFELET
                     RLIPDEQAIIEQTLCELVDEMSCHLVLTTGGTGPARRDVTPDATLAVADREMPGFGEQ
                     MRQISLHFVPTAILSRQVGVIRKQALILNLPGQPKSIKETLEGVKDAEGNVVVHGIFA
                     SVPYCIQLLEGPYVETAPEVVAAFRPKSARREVSE"
     gene            complement(6934..7887)
                     /gene="tal"
                     /locus_tag="NBI74_003216"
     CDS             complement(6934..7887)
                     /gene="tal"
                     /locus_tag="NBI74_003216"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HCD1933500.1"
                     /translation="MTDKLTSLRQYTTVVADTGDIAAMKLYQPQDATTNPSLILNAAQ
                     IPEYRKLIDDAVAWAKQQSNDRAQQIVDATDKLAVNIGLEILKLVPGRISTEVDARLS
                     YDTEASIAKAKRLIKLYNDAGISNDRILIKLASTWQGIRAAEQLEKEGINCNLTLLFS
                     FAQARACAEAGVFLISPFVGRILDWYKANTDKKEYAPAEDPGVVSVSEIYQYYKEHGY
                     ETVVMGASFRNIGEILELAGCDRLTIAPALLKELAESEGAIERKLSYTGEVKARPARI
                     TESEFLWQHNQDPMAVDKLAEGIRKFAVDQEKLEKMIGDLL"
     gene            8166..9596
                     /locus_tag="NBI74_003217"
     CDS             8166..9596
                     /locus_tag="NBI74_003217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
                     /protein_id="HCD1933501.1"
                     /translation="MPDFFSFINSVLWGSVMIYLLFGAGCWFTFRTGFVQFRYIRQFG
                     KSLKNSIHPQPGGLTSFQSLCTSLAARVGSGNLAGVALAITAGGPGAVFWMWVAAFIG
                     MATSFAECSLAQLYKERDVNGQFRGGPAWYMARGLGMRWMGVLFALFLLIAYGIIFSG
                     VQANAVARALSFSFDFPPLVTGIILAVFALLAITRGLHGVARLMQGFVPLMAIIWVLT
                     SLVICVMNIGQLPHVIWSIFESAFGWQEAAGGAAGYTLSQAITNGFQRSMFSNEAGMG
                     STPNAAAAAASWPPHPAAQGIVQMIGIFIDTLVICTASAMLILLAGNGTTYMPLEGIQ
                     LIQKAMRVLMGSWGAEFVTLVVILFAFSSIVANYIYAENNLFFLRLNNPKAIWCLRIC
                     TFATVIGGTLLSLPLMWQLADIIMACMAITNLTAILLLSPVVHTIASDYLRQRKLGVR
                     PVFDPLRYPDIGRQLSPDAWDDVSQE"
     gene            9666..10442
                     /gene="yaaA"
                     /locus_tag="NBI74_003218"
     CDS             9666..10442
                     /gene="yaaA"
                     /locus_tag="NBI74_003218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HCD1933502.1"
                     /translation="MLILISPAKTLDYQSPLPTTRYTLPELLDNSQQLIHEARKLTPP
                     QISTLMRISDKLAGINAARFHDWQPDFTPENARQAILAFKGDVYTGLQAETFSEDDFD
                     FAQQHLRMLSGLYGVLRPLDLMQPYRLEMGIRLENARGKDLYQFWGDIITNKLNEALA
                     AQGDNVVINLASDEYFKSVKPKKLNAEIIKPVFLDEKNGKFKIISFYAKKARGLMSRF
                     IIENRLTKPEQLTGFNSEGYFFDEASSSNGELVFKRYEQR"
     gene            complement(10482..10778)
                     /locus_tag="NBI74_003219"
     CDS             complement(10482..10778)
                     /locus_tag="NBI74_003219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein"
                     /protein_id="HCD1933503.1"
                     /translation="MKKMQSIVLALSLVLVAPMAAQAAEITLVPSVKLQIGDRDNRGY
                     YWDGGHWRDHGWWKQHYEWRGNRWHPYGPPPSPRHNKHNDHRGDHRPGPDKHHR"
     gene            complement(10993..12279)
                     /gene="thrC"
                     /locus_tag="NBI74_003220"
     CDS             complement(10993..12279)
                     /gene="thrC"
                     /locus_tag="NBI74_003220"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HCD1933504.1"
                     /translation="MKLYNLKDHNEQVSFAQAVTQGLGKNQGLFFPHDLPEFSLTEID
                     EMLKLDFVTRSAKILSAFIGDEIPQEILEERVRAAFVFPAPVANVESDVGCLELFHGP
                     TLAFKDFGGRFMAQMLTHIAGDKPVTILTATSGDTGAAVAHAFYGLPNVKVVILYPRG
                     KISPLQEKLFCTLGGNIETVAIDGDFDACQALVKQAFDDEELKVALGLNSANSINISR
                     LLAQICYYFEAVAQLPQEARNQLVVSVPSGNFGDLTAGLLAKSLGLPVKRFIAATNVN
                     DTVPRFLHDGQWSPKATQATLSNAMDVSQPNNWPRVEELFRRKIWQLKELGYAAVDDE
                     TTQQTMRELKELGYTSEPHAAVAYRALRDQLNPGEYGLFLGTAHPAKFKESVEAILGE
                     TLDLPKELAERADLPLLSHNLPADFAALRKLMMNHQ"
     gene            complement(12280..13212)
                     /gene="thrB"
                     /locus_tag="NBI74_003221"
     CDS             complement(12280..13212)
                     /gene="thrB"
                     /locus_tag="NBI74_003221"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HCD1933505.1"
                     /translation="MVKVYAPASSANMSVGFDVLGAAVTPVDGALLGDVVTVEAAETF
                     SLNNLGRFADKLPSEPRENIVYQCWERFCQELGKQIPVAMTLEKNMPIGSGLGSSACS
                     VVAALMAMNEHCGKPLNDTRLLALMGELEGRISGSIHYDNVAPCFLGGMQLMIEENDI
                     ISQQVPGFDEWLWVLAYPGIKVSTAEARAILPAQYRRQDCIAHGRHLAGFIHACYSRQ
                     PELAAKLMKDVIAEPYRERLLPGFRQARQAVAEIGAVASGISGSGPTLFALCDKPDTA
                     QRVADWLGKNYLQNQEGFVHICRLDTAGARVLEN"
     gene            complement(13214..15676)
                     /gene="thrA"
                     /locus_tag="NBI74_003222"
     CDS             complement(13214..15676)
                     /gene="thrA"
                     /locus_tag="NBI74_003222"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase I"
                     /protein_id="HCD1933506.1"
                     /translation="MRVLKFGGTSVANAERFLRVADILESNARQGQVATVLSAPAKIT
                     NHLVAMIEKTISGQDALPNISDAERIFAELLTGLAAAQPGFPLAQLKTFVDQEFAQIK
                     HVLHGISLLGQCPDSINAALICRGEKMSIAIMAGVLEARGHNVTVIDPVEKLLAVGHY
                     LESTVDIAESTRRIAASRIPADHMVLMAGFTAGNEKGELVVLGRNGSDYSAAVLAACL
                     RADCCEIWTDVDGVYTCDPRQVPDARLLKSMSYQEAMELSYFGAKVLHPRTITPIAQF
                     QIPCLIKNTGNPQAPGTLIGASRDEDELPVKGISNLNNMAMFSVSGPGMKGMVGMAAR
                     VFAAMSRARISVVLITQSSSEYSISFCVPQSDCVRAERAMQEEFYLELKEGLLEPLAV
                     TERLAIISVVGDGMRTLRGISAKFFAALARANINIVAIAQGSSERSISVVVNNDDATT
                     GVRVTHQMLFNTDQVIEVFVIGVGGVGGALLEQLKRQQSWLKNKHIDLRVCGVANSKA
                     LLTNVHGLNLENWQEELAQAKEPFNLGRLIRLVKEYHLLNPVIVDCTSSQAVADQYAD
                     FLREGFHVVTPNKKANTSSMDYYHLLRHAAEKSRRKFLYDTNVGAGLPVIENLQNLLN
                     AGDELMKFSGILSGSLSYIFGKLDEGMSFSEATTLAREMGYTEPDPRDDLSGMDVARK
                     LLILARETGRELELADIEIEPVLPAEFNAEGDVAAFMANLSQLDDLFAARVAKARDEG
                     KVLRYVGNIDEDGVCRVKIAEVDGNDPLFKVKNGENALAFYSHYYQPLPLVLRGYGAG
                     NDVTAAGVFADLLRTLSWKLGV"
     misc_feature    complement(15703..15822)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00506"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Thr leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00506"
     gene            complement(15758..15823)
                     /gene="thrL"
                     /locus_tag="NBI74_003223"
     CDS             complement(15758..15823)
                     /gene="thrL"
                     /locus_tag="NBI74_003223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thr operon leader peptide"
                     /protein_id="HCD1933507.1"
                     /translation="MKRISTTITTTITITTGNGAG"
     gene            complement(16037..16723)
                     /locus_tag="NBI74_003224"
     CDS             complement(16037..16723)
                     /locus_tag="NBI74_003224"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HCD1933508.1"
                     /translation="MRITIILVAPARAENIGAAARAMKTMGFSELRIVDSQAHLEPAT
                     RWVAHGSGDIIDNIKVFPTLAESLHDVDFTVATTARSRAKYHYYATPVELVPLLEEKS
                     SWMSHAALVFGREDSGLTNEELALADVLTGVPMVADYPSLNLGQAVMVYCYQLATLIQ
                     QPAKSDTTADQHQLQALRERAMTLLTTLAVADDIKLVDWLQQRLGLLEQRDTAMLHRL
                     LHDIEKNITK"
BASE COUNT         3960 a         4469 c         4157 g         4333 t
ORIGIN      
        1 aggaattaat acaccagaca attcattttt attaagaatg accaggccag tatatttaaa
       61 gatgactatc gaagcgtgat gaagatcgca gattactttt gcgaacgggc ggagggatat
      121 cgtgtttcag aaaatgtgtc tgttttaaat atcacccttt gatttatagg catttttcat
      181 gatgaactta gcattggctg ctgaacgtat ttctttgtct ctctatagta ttacactaac
      241 ctttaataat taatttcctt tagccattca cttttgatag aggaaaacac cttttttgtg
      301 aatccctgga tatatttaag ttaaataaat tgatgtattg aaatattatt ccgcaaaaat
      361 gcgaggcgtc ttcagaaaaa gaattgataa aacagaagtg aattaaaagc gcagtataat
      421 agcggcgggt gcttgaggct gtctgtctca ggcattggct gaacggcaga tagagaaaag
      481 ccccgagtga tattttacca tcaacccgag gccccctata tgcagaacac atgtagagtg
      541 cctcttactg accgaaaggt caaggagaag agagcaatga agtagcataa ggcgatgatt
      601 gtcgccctga tcgtcatctg tatcaccgcc gtagtggcgg cgctggtaac gagaaaagac
      661 ctctgtgagg ttcacatccg aactggccag acggaggttg ctgttttcac ggcttacgaa
      721 tccgagtaag agcaacggcg gggagtgatc cccgccactt tacaggtgct cgcatatctt
      781 caacgcaccc ttttttttac ctggcctgcc cacgggcagg cttttgggga ggttagcgag
      841 tcaggtcgtc gaaaaacttc ttcacaccat caaagaagct ctttgagcgc gggctgttgt
      901 gctcgccggt tgggccacca aagctttctt gcagctcttg cagcagctgt ttctgcttct
      961 cgttcaggcc taccggtgtt tcgacgacaa cgcggcacag caaatcaccc tgtgcgccgc
     1021 cgcggacaga cttgacgcct ttaccacgca tacggaacag cttaccggtc tgggtttcgc
     1081 caggaacttt cagtttaacg cgaccatcaa gggtcggcac ttcgatttcg ccgcccagcg
     1141 ccgccatagc gaagttgatc gggacttcgc aatacaggtt gttgccttca cgctcgaaaa
     1201 tcgggtgctg tttaacctga acctgaacgt acaggtcgcc tgccggtgcg ccgtgttcgc
     1261 ccgcttcacc ttcgcccgca agacggatgc ggtctccagt gtccacccct gccgggattt
     1321 taacggacag cgttttgctg cgctcaacac gaccatgacc atgacatttg ttgcacggat
     1381 ctttgatcag cgtaccgcgg ccctgacagt gtggacaggt ctgctgcacg gcaaagaaac
     1441 cctggcgcat ctgcacctgg ccagaaccat gacaggtcgg acaggtctgc ggctgtgtac
     1501 ctggttttgc accgctaccg tggcaaacgt cacactcttc cagagtcgga atgcggatct
     1561 ctttggtcac gccacgtaca gcttcttcga gggtgagctc catgttatag cgtaaatcag
     1621 caccgcgcgc cgcacgttga cgaccacgtc cgccgccaaa aatatcgccg aaaacgtcac
     1681 caaaaatatc gctgaagtct gcgccgccgc caaaaccgcc gccgcccatg ccgccttgct
     1741 caaacgcagc atgaccatac tgatcgtatg ccgcacgttt ttgcgagtcg gtcagaactt
     1801 cataagcttc cttgatctct ttaaatttcg cctcggcctc tttgtcaccc tggttacggt
     1861 ccgggtggta tttcatggcc aggcgtttgt aggccttttt gatttcacgc tcttccgctg
     1921 ttttggaaac gcctaaaatc tcgtaataat cttgcttagc catctttttt gatttgcccc
     1981 tagatgaatg cacgggcgga gaggaaattc cccttcgccc gtgtcagtat aattacccgt
     2041 ttatagggcg attatttttt gtctttgact tcttcaaatt cagcgtcgac aacatcgtca
     2101 tctttcgcgt tgtttgcaga agcatcagca ccggcagtct gctgctgggc atgttgctgc
     2161 tgggcgattt ccatcagttt ctgggaaacc tgtgccagtt cctgcatttt cgcttcgata
     2221 gcggctttgt cttcaccttt cagagcagtt tccagtgcag tcagcgcaga ctcgatagca
     2281 gttttgtcgt cagccggcag tttgtcgcct gcttcttcaa cctgcttacg ggtgctgtgc
     2341 agcagatggt cgccctggtt gcgagtctgt accagctctt caaacttacg gtcagcttcg
     2401 gcgttagctt ctgcgtcgcg taccattttc tggatttcat cttcgttcag accagaagaa
     2461 gctttgatag tgatcttctg ctctttaccg ctgtttttgt ctttcgcgga aacgtgcagg
     2521 ataccgtcag catcgatatc gaaggtaact tcgatctgcg gcatgccgcg cggtgccggg
     2581 ttgataccat ccaggttgaa ctgacccaga gatttgttat cagccgcacg tttacgttca
     2641 ccctgcagca catggatggt taccgcagac tggttgtctt cagcggtaga gaacacctgg
     2701 ctgtgcttgg tcgggatagt ggtgtttttc gcgatcagcg tggtcatcac accgcccatg
     2761 gtttcgatac ccagagacag cggggtaacg tccagcagca gtacgtcttt tacgtcacca
     2821 gtcagaacac caccctgaac agcagcaccg atggctacag cttcgtccgg gttaacatct
     2881 ttacgcggct ctttaccaaa gaattcagca actttcttct gaaccattgg catacgagtc
     2941 tgaccaccaa cgaggataac gtcgtcgata tcagatacgg acaggccagc gtcctgcagt
     3001 gcaactttca gcggctcaat ggaacggttt accagatctt caaccaggct ttccagtttc
     3061 gcacgagtca ctttgatgtt catgtgtttc ggaccggtcg cgtctgcagt gatgtacggc
     3121 aggttaacgt cggtctgctg agcggaagac agttcgattt tcgctttttc tgccgcttct
     3181 ttcaggcgct gcattgccag cggatcgttg cgcaggtcaa tgccctgatc tttcttgaat
     3241 tcttcaacca gatagttgat cagacggctg tcgaagtctt caccacccag gtgggtatca
     3301 ccgttggttg ccagaacttc gaaggttttt tcgccgtcaa cttcgtcgat ttcgataatg
     3361 gaaatatcga aagtaccacc acccaggtca taaaccgcga tagtacggtt gccagtacct
     3421 ttgtccagac cgtaagccag cgcagctgcg gtcggttcgt tgatgatacg ttttacttcc
     3481 agaccagcga tacggcctgc gtctttggtt gcctgacgct gagcatcgtt aaagtatgcc
     3541 ggtacggtaa taacagcttc agttaccggt tcacccaggt aatcttcagc ggttttcttc
     3601 atttttttca gcacttcagc agagatctgc ggcggtgcca ttttctggcc tttaacttcg
     3661 acccatgcgt cgccgttatc agcagcaata attttgaacg gcatgatgga aacatcacgc
     3721 tgtacttctt cgtcctggaa gcggcggcca atcaggcgtt taatcgcaaa cagggtgttt
     3781 tgcgggttcg tcactgcctg acgtttagcc ggctgaccaa ccagagtttc accatcctgg
     3841 gtataggcaa tgatagaagg cgtggtgcga tcgccttcgg cgttctccag tacacgagga
     3901 gtggtgccat ccataatcgc tacacaagag ttggtagtac ccaggtcgat accaattatt
     3961 ttacccatct aaacgtctcc actatatatt cggtcatcat gtggttgtga gtctgtaata
     4021 ggggcgaaac gtctggtttc aactgcccaa tttcattttt ttgcagactc actcactgcg
     4081 gttgactact aaatggggtc gtaacccacg tcatcaaggg ggagatgcaa aaattttttg
     4141 tgcttttgcg cagaaatttc gcgatatggc aggacaagac gtgaaactga cagcaatttt
     4201 attcatataa agaatgaatc gcagcaaggc attcatcaaa aaattgtaat aaaaagaaaa
     4261 tattacgtgc ctgaaacttc tctttatcag cggtaaacta gtttcatccc cttacccctc
     4321 cccaccaaga gaatccttat gaaatccgtt attacgattt ccgccagcct ggcgattagc
     4381 ctgatgctgt gctgcacggc gcaggcaaac gaccataaaa tcctcggcgt cattgcaatg
     4441 ccgcgaaacg aaaccaacga tctggcgctg aaacttcctg tttgtcgcat tgttaaacgc
     4501 atacaactct cagccgacca tggcgatttg cagttaagcg gtgcatcgat ttatttcaaa
     4561 gccacccgaa gcgccagtca gaccctgaat attccttcag aaataaaaga agggcaaacc
     4621 actgactgga tcaacattaa cagcgataac gacaacaaac gctgcgtctc aaaaatcacc
     4681 ttttccggtc atacagtgaa ctcatcggat atggccacgc tgaaaattat cggcgacgac
     4741 taacgccaga atatagaaac cacaaaaaat gaatgttaat tacctgaatg attcagatct
     4801 ggattttctc cagcattgta gtgaggaaca gttggcaaat ttcgcccggc tgctcaccca
     4861 taatgaaaaa ggcaaaactc gcctctccag cgtactgatg cgcaacgaac tgtttaaatc
     4921 gatggaagga catcccgagc gacaccgccg caactggcag ctgattgccg gagaattaca
     4981 gcattttggt ggcgatagta tcgccaacaa actgcgcgga cacggtaaat tgtatcgcgc
     5041 cattttgctc gatgtttcaa agcgattgaa gctgaaagcc gacaaagaga tgtctacgtt
     5101 tgaaattgag caacagttac tggaacaatt tctgcgtaat acctggaaga aaatggacga
     5161 ggaacataag caggagtttc tgcacgcggt cgatgccagg gtgaatgagc tggaagagct
     5221 gctgccgctg ctgatgaaag acaaattatt ggcaaaaggt gtgtcgcatt tgctttccag
     5281 ccaattgacc cgcattttac gcacccacgc agcaatgagc gtgcttgggc atggtttgct
     5341 gcgcggcgcg ggtctgggag gccctgtggg cgcagcatta aatggggtta aagcggtcag
     5401 cggcagtgcc tatcgcgtga caattccagc cgtactgcaa atcgcctgcc tgcgccggat
     5461 ggttagcgcc actcaggtct gataccggaa accggggaaa attttcattt tcccctcaaa
     5521 agatcgtaga cactgcccca ctggctgatt attatgccgc gccctgaaaa cactacagtt
     5581 attcagggaa attatttcac cattcattcg atgatgattt ttgaggaatt atgggcaaca
     5641 ctaagttggc taatccggca ccgctgggcc tgatgggctt cggcatgacc accattctgc
     5701 ttaacctgca caacgtgggt tattttgctc tggacggtat tattcttgcc atgggcattt
     5761 tctacggcgg catcgcgcaa atttttgccg gtctgctgga gtacaaaaaa ggcaacactt
     5821 tcggtttaac cgcattcacc tcttacggtt ctttctggct gacgctggtt gcaattctgc
     5881 tgatgccgaa actggggctg accgatgcgc caaatgcaca gttccttggt gtctacctgg
     5941 gtctgtgggg catatttacg ctgtttatgt tcttcggcac gctgaaaggc gcacgcgttc
     6001 tgcaattcgt tttctttagc ctgaccgtgc tgtttgccct gctggcgatc ggtaacattg
     6061 ccggtaacgc cgcgatcatc cactttgccg gctggattgg tcttatctgc ggtgccagcg
     6121 caatctatct ggcgatgggt gaagtgctga acgagcagtt tggtcgcacc gttctgccga
     6181 ttggtgaatc ccactaattg ttttgagatc cccccgctcg gggggatttt tttattcgct
     6241 aacttcgcgt cttgcactct tcggtctgaa tgctgcaacc acttccggtg ccgtttcaac
     6301 gtatggccct tccagcaact gaatgcagta cggtacgctg gcaaaaatac cgtgcaccac
     6361 aacgttaccc tcagcgtcct tcacaccttc cagcgtctct ttaatagact tcggttgacc
     6421 gggtaagtta aggatcagcg cctgtttgcg aatcaccccc acctgacgcg aaaggatcgc
     6481 agttggtaca aaatgcaggc tgatctggcg catctgttca ccaaagcctg gcatctcgcg
     6541 gtccgctact gccagcgtcg catcgggcgt tacgtcacga cgcgccgggc cagttccgcc
     6601 cgtggtaagc accagatggc aactcatttc atccaccagc tcacacaacg tttgctcgat
     6661 gatcgcctgc tcatcgggaa ttaagcgggt ttccagttca aacggcgtgg ttagcgccga
     6721 tgtcagccat tcttccagcg cagggatgcc tttatcctga taaacgccgc tggatgcgcg
     6781 atcagagatg gaaactaagc caatacgtaa agtattcatc aagatttttc cgtttaaaca
     6841 gtctcgttaa acagaatgat acactgcgaa gggagtgaca gacaggtttc agaagaggta
     6901 gcgtgaccga cttcccggtc acgctaagaa tgattacagc agatcgccga tcattttttc
     6961 cagtttttcc tggtcaacag caaacttacg gataccttcc gccagtttat ctactgccat
     7021 tggatcctgg ttgtgctgcc acaggaactc ggactcagtg atacgcgccg gacgcgcttt
     7081 cacttcacca gtgtaagaca gtttacgttc gatagcccct tcgctctccg ccagctcttt
     7141 cagcagtgcc ggtgcgatgg tcagacggtc gcagcctgcc agttccagaa tttcgccgat
     7201 gttacggaag cttgcgccca taaccacggt ttcataaccg tgctctttgt agtactggta
     7261 gatttcagat acagaaacca cgcctggatc ttctgccgga gcgtactctt tcttatcggt
     7321 attcgctttg taccagtcaa gaatacggcc aacaaacggc gagatcagga acacgcccgc
     7381 ttccgcacaa gcacgagcct gagcgaagga gaacagcagg gtcaggttac agttgatgcc
     7441 ttctttttcc agctgttccg cagcacggat accctgccag gtagaagcca gtttgatcag
     7501 aatacgatcg ttgctaatac ctgcatcgtt gtagagtttg atcaggcgtt ttgctttcgc
     7561 aattgacgct tcggtgtcat aggaaagacg cgcatcaact tcagttgaga tacggcccgg
     7621 aaccagtttc aggatttcca gaccaatatt tactgccagt ttgtcggtcg cgtccacgat
     7681 ctgctgcgcg cgatcgttgc tctgctgttt cgcccaggcg acagcatcat caatcaactt
     7741 acggtattcc ggaatctgcg ctgcgttaag aatgagagaa gggttggttg tggcatcctg
     7801 cggttgatac agcttcattg ccgcgatgtc cccagtgtcg gccactacgg tggtgtactg
     7861 acgaagggag gtcaatttgt ccgtcatgat agtatttctc tttaaacagc ttgttagggg
     7921 gatgtaaccg gtctgccctg atgatatcac gacgcaacgt cagcgcaacc gccgacaaga
     7981 cggttatcgc caggcgagac tgtttcagat ttctgacact gcgttgatat cgcccgccat
     8041 ttttatacaa aacctcatgt atgctacgca gaagttatca agtacctcgt agcgtatata
     8101 cttcttaaac aattggtaac gtttacacag gaaagtcatc gcgaccggca ataagaggga
     8161 tatgcatgcc agattttttc tcctttatta acagcgtcct ttggggatcg gtaatgattt
     8221 acctgctctt cggcgctggt tgttggttca cttttcgcac cggatttgtg cagtttcgct
     8281 acatccgcca gtttggcaaa agtcttaaaa atagcattca tccacagcca ggcggtttaa
     8341 cctcatttca gtcattgtgt accagtcttg cggcgcgcgt gggtagcggc aacctggccg
     8401 gcgttgcgct ggctattacc gccggtggac caggtgccgt cttctggatg tgggttgccg
     8461 cgtttatcgg catggcgacc tcgtttgccg aatgttccct tgcacaactt tataaagaac
     8521 gtgacgttaa tgggcagttt cgtggcggac cggcatggta tatggcgcgc gggctgggga
     8581 tgcgctggat gggcgttctg ttcgccctct ttttgctcat cgcctacggc ataattttca
     8641 gcggagttca ggcgaacgcc gttgcccgag ccctgagttt ttcttttgat tttcccccgc
     8701 tggtgacagg cattattctt gctgtctttg ctctgctggc aatcactcgc ggtcttcatg
     8761 gcgtcgcccg gctcatgcaa gggtttgtcc cgttgatggc gataatctgg gtactgacca
     8821 gcctggtaat ttgcgtaatg aatatcgggc aacttcccca cgtcatttgg tctatttttg
     8881 agagtgcttt tggctggcag gaagcggcag gcggcgcggc gggatatacc ttaagccagg
     8941 cgattactaa cggttttcag cgcagtatgt tttccaatga ggcgggaatg gggtcgacgc
     9001 caaacgcggc agcggcggct gcgtcctggc ctccgcatcc cgcagcgcaa ggaattgtcc
     9061 agatgattgg catttttatc gacaccctgg tcatctgtac ggcaagcgcc atgctgatat
     9121 tactggcggg taacggcaca acctacatgc cgctggaagg tattcagctt atccagaagg
     9181 cgatgcgggt gttaatgggt tcctggggtg cggagtttgt taccctcgtg gttattctgt
     9241 ttgccttcag ctccatcgtt gccaactaca tttatgccga aaacaatctc ttcttcttac
     9301 gcctgaacaa ccctaaagcg atctggtgtc tgcgaatctg caccttcgca acggtcatcg
     9361 gcggcacctt gctaagtctt ccgctgatgt ggcaactggc agatatcatt atggcctgca
     9421 tggctattac caatttgacc gccatattac tgctctcgcc tgtggttcat accattgcca
     9481 gtgattatct acgccagcgt aaactcggcg tgcgcccggt gtttgatccg ttgcgttatc
     9541 cggatatcgg tcgccagctt tctccggacg cgtgggatga tgtttcgcag gagtaatcac
     9601 aactatcgat caactcattc tctttttttg ctaaagtcgg cataaatttc ctgcaaggac
     9661 tggatatgct gattcttatt tcacctgcga aaacgctcga ttaccaaagc ccgttgccca
     9721 ccacgcgcta tacgctgccg gagctgttag acaattccca gcagttgatc catgaagcgc
     9781 ggaaactgac gcctccgcag attagcacgc tgatgcgcat cagcgacaaa ctggcgggta
     9841 tcaacgccgc tcgctttcat gactggcagc cagatttcac gccggagaat gcccgccagg
     9901 cgattctggc gtttaaaggt gatgtctaca ccggcttgca ggccgaaacc ttcagcgaag
     9961 acgatttcga ttttgcccaa cagcatttgc gaatgctttc cggcttgtat ggcgtactgc
    10021 gcccgctcga tttaatgcag ccttatcgtc tggaaatggg gatccgtctt gagaatgccc
    10081 gagggaaaga tctgtatcaa ttctggggag atatcatcac caacaagctg aacgaggcgc
    10141 tcgcagcaca aggcgataat gtggtgataa atctggcgtc agatgagtat tttaaatcgg
    10201 tgaagccgaa gaaattgaat gccgagatta tcaagccggt gttcctcgat gagaagaacg
    10261 gcaaatttaa gatcatcagc ttctacgcta agaaagcacg cggtctgatg agtcgtttca
    10321 ttattgaaaa tcggctgacc aaaccagagc aactgactgg ttttaatagc gaaggttact
    10381 tctttgatga agcttcctcc agcaatggtg aactggtgtt taaacgctac gagcagcgtt
    10441 aagtttgtag tcaatgccgg atggcaatcc ggcattgata tttagcgatg atgtttgtca
    10501 ggccccggac gatgatcgcc acgatgatca ttgtgcttgt tatggcgcgg cgatggcggc
    10561 ggtccatatg ggtgccagcg attgcctcgc cattcataat gttgtttcca ccagccgtgg
    10621 tcgcgccagt gaccgccatc ccagtaatag ccacgattat cacgatcgcc tatctgtaat
    10681 tttactgacg ggactaacgt aatttccgca gcctgtgctg ccatgggagc gaccagaacc
    10741 agggaaagtg cgagtacgat agattgcatc tttttcacag gttactcctt ttatcattgc
    10801 ccgttatggg tcgatatccg gagattacgc gatgagaacg gcggttgtta ttctccgcaa
    10861 tcctaatctc taagttaccg catttattga gaattcctcc tttttccctg tcgtttttct
    10921 ccatattttc ttcataaaaa aagccgggct gtataaaagc aaacccggcc tgattgagat
    10981 aatgaataga tattactgat gattcatcat caatttacgc aacgcagcaa aatcggcggg
    11041 cagattatgc gaaagcaagg gtaaatcggc acgttctgcc agctcttttg gcagatccaa
    11101 cgtttcaccg agaatcgctt ccacgctctc tttaaatttc gccggatgcg cggtgccgag
    11161 gaacaagcca tattcgcctg gattcaactg gtcacgcagc gcacgataag ctacggcagc
    11221 gtgcggctcc gaggtgtagc ccagttcttt taactcacgc attgtctgtt gcgtggtttc
    11281 atcatccacg gctgcataac ccagctcttt cagttgccag attttgcggc ggaacaactc
    11341 ttccacacgc ggccagttgt tcggctgact cacgtccatc gcgttggata acgtcgcctg
    11401 agtcgctttg ggggaccact gaccgtcgtg caggaaacgt ggcacggtat cgttcacgtt
    11461 ggtcgcagca ataaaacgtt tcaccggcag accgagtgac ttcgccagca gacccgccgt
    11521 caaatcgccg aagttaccgc tcggcaccga gacaaccagc tggttgcgcg cttcctgcgg
    11581 cagctgcgcc acggcttcaa agtagtagca aatctgcgcc agcaaacggc tgatgttaat
    11641 cgagttagcc gagtttaacc ccagcgccac tttcagctct tcatcatcaa acgcctgctt
    11701 caccagcgcc tgacaggcat cgaaatcgcc gtcgatggca acagtttcaa tattgccgcc
    11761 caatgtacag aacagttttt cttgcagtgg actgattttg cctcgtggat agaggataac
    11821 cactttcaca ttcggcaaac cgtagaaagc atgagccact gccgctccgg tatcaccgga
    11881 ggtcgcggtc agaatggtca ctggcttatc gcccgcaata tgggtcagca tttgtgccat
    11941 aaagcgaccg ccgaaatctt taaatgccag cgttggcccg tggaacaatt ccagacaacc
    12001 gacatcgctt tcaacattgg cgaccggagc cgggaagaca aacgccgcgc gtacgcgctc
    12061 ttccaggatt tcctgcggga tttcatcacc aataaacgcc gagaggatct tcgcactgcg
    12121 ggtgacaaaa tccagcttca gcatctcatc aatttcagtc aggctgaatt ccggcaggtc
    12181 gtgcgggaaa aacagcccct gatttttgcc caacccctgg gttacggctt gcgcaaagct
    12241 gacctgctca ttgtgatctt tcagattgta gagtttcatt tagttttcca gtactcgtgc
    12301 gcccgccgta tccagccggc aaatatgaac aaaaccttcc tgattttgca ggtagttctt
    12361 acccaaccag tcggcaacgc gctgggcggt atccggcttg tcacacagag cgaacaaagt
    12421 cgggccggag ccggagatac cgctcgctac cgcgccgatt tccgcgaccg cctgccgcgc
    12481 ctgccggaag cctggcagta accgttcacg gtagggttca gcgataacat ctttcatcag
    12541 cttcgcggca agctcaggct gacgggaata gcaggcgtga ataaagcctg ccagatgtcg
    12601 cccgtgcgca atgcaatcct ggcggcgata ctgcgccggt aaaatagccc tggcttctgc
    12661 cgtcgagact ttaatccccg gatacgccag cacccacagc cactcatcaa accctggcac
    12721 ttgctggctg atgatgtcgt tttcttcgat catcaactgc ataccaccga gaaaacacgg
    12781 tgccacgttg tcgtaatgaa tgctgccgga gatacgccct tccagctcgc ccatcaaagc
    12841 cagcaaacgg gtgtcattaa gcggcttgcc gcagtgttca ttcatcgcca tcagcgccgc
    12901 gaccactgag caggcgctgg agcctaagcc cgagccgatc ggcatattct tttccagggt
    12961 catcgccact ggaatttgct tacccagctc ctggcaaaaa cgctcccaac actgataaac
    13021 gatattttcc cgaggttctg acggcagctt atcggcaaag cgtccgaggt tattgagact
    13081 gaatgtctct gccgcctcaa ccgtgactac atctccgagc aatgcaccat caacgggtgt
    13141 caccgccgcc ccgagcacat cgaacccgac gctcatattg gcactggaag ccggggcata
    13201 aactttaacc atgtcagact cctaacttcc atgagagggt acgtagcaga tcggcaaaaa
    13261 caccggcagc tgtaacgtca ttgcccgcac catatccgcg cagcaccaac ggcagcggct
    13321 gataatagtg gctatagaag gccagggcgt tttcgccatt tttcactttg aacagcggat
    13381 cattaccatc cacttcggca atcttcacgc ggcagacgcc atcttcatca atattgccaa
    13441 catagcgcaa aacttttcct tcatcacggg ccttcgccac gcgcgcggca aagagatcgt
    13501 cgagctgtga cagattcgcc ataaaagcgg caacatcacc ctcggcgtta aactctgcgg
    13561 gcagcacagg ttcaatttca atatccgcca gctccagttc acgtcccgtt tcacgagcga
    13621 gaatcaatag tttacgcgcc acatccatac cagaaagatc atctcgcggg tccggttcgg
    13681 tataacccat ttcccgcgcc agcgtggtcg cctcggagaa actcatgcct tcgtctaact
    13741 tgccgaagat ataagaaagc gaaccagaaa gaatgccgga gaacttcatc aattcatcac
    13801 ctgcattgag cagattttgc aggttctcaa taaccggtaa tccagcccca acgttggtgt
    13861 catagaggaa tttacgccgc gatttttccg ccgcatgacg caacagatgg tagtaatcca
    13921 tcgacgaggt gttagccttt ttgttcggcg taacaacgtg gaaaccttcg cgcaggaagt
    13981 cggcatattg atccgccact gcctggctgg aggtgcagtc aacaatgacc gggttcaaca
    14041 gatgatattc tttcacgagg cgaattaagc gcccgagatt aaacggctct ttggcttgcg
    14101 ccagttcttc ctgccagttt tccagattaa ggccatgtac attggtgagc agtgccttcg
    14161 agttagcaac accgcagaca cgtaagtcga tatgtttatt cttcaaccag ctttgctgac
    14221 gcttcagttg ctccagcagc gcaccgccaa cgccaccgac gccaatcaca aacacttcga
    14281 taacctgatc ggtattgaac agcatctgat gagtaacgcg cacgccagtg gtcgcatcat
    14341 cgttattgac cacgacagag attgagcgtt cagaagatcc ctgagcaatg gcgacaatat
    14401 tgatgttggc gcgggccagc gcggcaaaga atttcgccga gatcccacgt aaggtgcgca
    14461 taccatcacc taccaccgag ataatggcca gccgttccgt caccgccaac ggctccagta
    14521 agccttcttt cagttccagg tagaactctt cctgcattgc ccgttcagct cgcacacagt
    14581 cgctttgcgg aacgcagaaa ctgatactgt attcggaaga tgattgcgta atcagcacca
    14641 cggaaatacg ggcgcgtgac atcgctgcaa agacgcgcgc cgccatgcca accatccctt
    14701 tcatccccgg gccggaaacg ctgaacattg ccatgttatt cagattggaa atgcccttga
    14761 ccggtaattc gtcttcatca cggctggcac caatgagcgt acctggtgct tgaggatttc
    14821 cggtattttt aatcaggcaa gggatctgga actgggcgat gggggtaatg gtgcgggggt
    14881 gaagaacttt agcgccgaag taagaaagct ccatcgcttc ctgataggac atcgacttca
    14941 acaacctcgc atcgggcacc tgacgcggat cgcaggtata aacaccgtca acatccgtcc
    15001 agatctcgca acaatcggcg cgtaaacagg ccgccagcac cgcagcggag tagtcggaac
    15061 cgttgcgtcc cagaaccacc agctcgcctt tttcattacc ggcagtgaaa ccagccatca
    15121 gcaccatgtg gtcagccgga atgcggcttg ccgcaatacg gcgggtggat tcagcaatat
    15181 caacggtaga ttcgaggtaa tgacccactg ccagcagttt ttcgaccgga tcgataacgg
    15241 taacgttgtg accacgcgct tctaacacgc cggccataat ggcgatcgac attttctcgc
    15301 cacggcaaat cagcgcagcg ttgatgctat ccgggcactg ccccaacaaa ctgatgccat
    15361 gcaggacatg ttttatttgg gcaaattcct ggtcgacgaa agttttcaat tgtgccagcg
    15421 gaaatcccgg ctgggcggcg gcgagtcccg tcagaagttc ggcaaaaata cgttcggcat
    15481 cgctgatatt gggtaaagca tcctggccgc taatagtttt ttcaatcatc gctaccagat
    15541 ggttggtaat tttggcgggg gcagagagga cggtcgccac ctgcccctgc ctggcattgc
    15601 tttccagaat atcggcaacc cgcagaaaac gttctgcatt tgccactgat gtaccgccga
    15661 acttcaacac tcgcatggtt gttacctcgt tacctttggt cgaaaaaaaa agcccgcact
    15721 gtcaggtgcg ggcttttttc tgtgtttcct gtacgcgtca gcccgcaccg ttacctgtgg
    15781 taatggtgat ggtggtggta atggtggtgc taatgcgttt catggatgtt gtgtactctg
    15841 taatttttat ctgtctgtgc gctatgccta tattggttaa agtatttagt gacctaagtc
    15901 aataaaattt taatttgatc acggcaggta accagttcag aagctgctat cagacactct
    15961 ttttttaatc cacacagaga catattgccc gttgcagtca gaatgaaaag ctgaaaatca
    16021 cttactaagg cgttttttat ttggtgatat ttttttcaat atcatgcagc aaacggtgca
    16081 acattgccgt gtctcgttgc tctaaaagcc ccaggcgttg ttgtaaccag tcgaccagtt
    16141 ttatgtcatc tgccactgcc agagtcgtca gcaatgtcat ggctcgttcg cgtaaagctt
    16201 gcagttgatg ttggtctgcc gttgtgtcac ttttcgccgg ttgttgtatt aatgttgcta
    16261 attgatagca atagaccatc accgcctgcc ccagattgag cgaaggataa tccgccacca
    16321 tcggcacacc agtaagaacg tcagccaacg ctaactcttc gttagtcagc ccggaatctt
    16381 cgcgaccaaa caccagcgcg gcatggctca tccatgaaga tttttcctct aacagcggca
    16441 ccagttctac tggcgtagcg tagtaatgat atttcgcccg actgcgcgca gtggtggcga
    16501 cagtgaaatc gacatcgtgt aacgattcag ccagtgtcgg gaaaacttta atattatcaa
    16561 taatatcacc agatccatgt gcgacccagc gggtggctgg ctccaggtgt gcctgactat
    16621 cgacaatccg cagctcgcta aaccccatcg ttttcattgc ccgcgccgct gccccaatat
    16681 tttctgctct ggcgggtgcg accagaataa tcgttatacg catattgcca ctcttcttga
    16741 tcaaataacc gcgaaccggg tgatcactgt caacttatta cgcggtgcga atttacaaat
    16801 tcttaacgta agtcgcagaa aaagcccttt acttagctta aaaaaggcta aactatttcc
    16861 tgactgtact aacggttgaa ttgttaaaaa atgctacata tccttctgtt tacttagga
//
LOCUS       DAHHTG010000096        16849 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.096, whole genome shotgun sequence.
ACCESSION   DAHHTG010000096 DAHHTG010000000
VERSION     DAHHTG010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16849)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16849)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16849
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.096"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            265..351
                     /gene="leuL"
                     /locus_tag="NBI74_003225"
     CDS             265..351
                     /gene="leuL"
                     /locus_tag="NBI74_003225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leu operon leader peptide"
                     /protein_id="HCD1933509.1"
                     /translation="MTHIVRFIGLLLLNASSLRGRRVSGIQH"
     gene            443..2014
                     /gene="leuA"
                     /locus_tag="NBI74_003226"
     CDS             443..2014
                     /gene="leuA"
                     /locus_tag="NBI74_003226"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HCD1933510.1"
                     /translation="MSQQVIIFDTTLRDGEQALQASLSVKEKLQIALALERMGVDVME
                     VGFPVSSPGDFESVQTIARQVKNSRVCALARCVEKDIDVAAESLKVAEAFRIHTFIAT
                     SPMHIATKLRSTLDEVIERAIYMVKRARNYTDDVEFSCEDAGRTPIADLARVVEAAIN
                     AGATTINIPDTVGYTMPFEFAGIISGLYERVPNIDKAIISVHTHDDLGLAVGNSLAAV
                     HAGARQVEGAMNGIGERAGNCSLEEVIMAIKVRKDILNVHTAINHQEIWRTSQLVSQI
                     CNMPIPANKAIVGSGAFAHSSGIHQDGVLKNRENYEIMTPESIGLNQIQLNLTSRSGR
                     AAVKHRMDEMGYKESEYNLDNLYDAFLKLADKKGQVFDYDLEALAFIGKQQEEPEHFR
                     LDYFSVQSGSNDIATAAVKLACGEEVKAEAANGNGPVDAVYQAINRITDYNVELVKYS
                     LTAKGHGKDALGQVDIVANYNGRRFHGVGLATDIVESSAKAMVHVLNNIWRAAEVEKE
                     LQRKAQHNENNKETV"
     gene            2014..3105
                     /gene="leuB"
                     /locus_tag="NBI74_003227"
     CDS             2014..3105
                     /gene="leuB"
                     /locus_tag="NBI74_003227"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HCD1933511.1"
                     /translation="MSKNYHIAVLPGDGIGPEVMTQALKVLDAVRNRFAMRITTSHYD
                     VGGAAIDNHGQPLPPATVEGCEQADAVLFGSVGGPKWEHLPPDQQPERGALLPLRKHF
                     KLFSNLRPAKLYQGLEAFCPLRADIAANGFDILCVRELTGGIYFGQPKGREGSGQYEK
                     AFDTEVYHRFEIERIARIAFESARKRRHKVTSIDKANVLQSSILWREIVNEIATEYPD
                     VELAHMYIDNATMQLIKDPSQFDVLLCSNLFGDILSDECAMITGSMGMLPSASLNEQG
                     FGLYEPAGGSAPDIAGKNIANPIAQILSLALLLRYSLDADDAASAIERAINRALEEGI
                     RTGDLARGAAAVSTDEMGDIIARYVAEGV"
     gene            3108..4508
                     /gene="leuC"
                     /locus_tag="NBI74_003228"
     CDS             3108..4508
                     /gene="leuC"
                     /locus_tag="NBI74_003228"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HCD1933512.1"
                     /translation="MAKTLYEKLFDAHVVYEAENETPLLYIDRHLVHEVTSPQAFDGL
                     RAHGRPVRQPGKTFATMDHNVSTQTKDINACGEMARIQMQELIKNCKEFGVELYDLNH
                     PYQGIVHVMGPEQGVTLPGMTIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTLKQ
                     GRAKTMKIEVQGKAAPGITAKDIVLAIIGKTGSAGGTGHVVEFCGEAIRDLSMEGRMT
                     LCNMAIEMGAKAGLVAPDETTFNYVKGRLHAPKGKDFDDAVAYWKTLQTDEGATFDTV
                     VTLQAEEISPQVTWGTNPGQVISVNDNIPDPASFADPVERASAEKALAYMGLKPGIPL
                     TEVAIDKVFIGSCTNSRIEDLRAAAEIAKGRKVAPGVQALVVPGSGPVKAQAEAEGLD
                     KIFIEAGFEWRLPGCSMCLAMNNDRLNPGERCASTSNRNFEGRQGRGGRTHLVSPAMA
                     AAAAVTGHFADIRNIK"
     gene            4519..5124
                     /gene="leuD"
                     /locus_tag="NBI74_003229"
     CDS             4519..5124
                     /gene="leuD"
                     /locus_tag="NBI74_003229"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HCD1933513.1"
                     /translation="MAEKFIKHTGLVVPLDAANVDTDAIIPKQFLQKVTRTGFGAHLF
                     NDWRFLDEKGQQPNPDFVLNFPQYQGASILLARENFGCGSSREHAPWALTDYGFKVVI
                     APSFADIFYGNSFNNQLLPVKLSDAEVDELFALVKANPGIHFDVDLEAQEVKAGEKTY
                     RFTIDAFRRHCMMNGLDSIGLTLQHDDAIASYEEKQPAFMR"
     gene            complement(5231..5362)
                     /gene="sgrT"
                     /locus_tag="NBI74_003230"
     CDS             complement(5231..5362)
                     /gene="sgrT"
                     /locus_tag="NBI74_003230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001248776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake inhibitor SgrT"
                     /protein_id="HCD1933514.1"
                     /translation="MRQFYRHYFTATAKLCWLRWLSVPQRLTMLEELMQWEGSHSDY"
     gene            5451..7106
                     /gene="sgrR"
                     /locus_tag="NBI74_003231"
     CDS             5451..7106
                     /gene="sgrR"
                     /locus_tag="NBI74_003231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SgrR"
                     /protein_id="HCD1933515.1"
                     /translation="MPSARLQQQFIRLWQCCEGKSQETTLNELAALLSCSRRHMRTLL
                     NTMQDRGWLTWEAEVGRGKRSRLTFLYTGLALQQQRAEDLLEQDRIDQLVQLVGDKAT
                     VRQMLVSHLGRSFRQGRHILRVLYYRPLRNLLPGSALRRSETHIARQIFSSLTRINEE
                     NGELEADIAHHWQQISPLHWRFFLRPGVHFHHGRELEMDDVIASLKRINTLPLYSHIA
                     DIVSPTPWTLDIHLTQPDRWLPLLLGQVPAMILPREWETLSNFASHPIGTGPYAVIRN
                     STNQLKIQAFDDFFGYRALIDEVNVWVLPEIADEPAGGLMLKGPQGEEQEIESRLEEG
                     CYYLLFDNRTHRGANQQVRDWVSYVLSPTNLVYFAEEQYQQLWFPAYGLLPRWHHARP
                     THCEKPAGLEHLTLTFYQDHIEHRVIAGIMQQILASHQVTLEIKEISYDQWHEGEIES
                     DIWLNSANFTLPLDFSLFAHLCEVPLLQHCIPIDWQADAARWRNGEMNLANWCQQLVA
                     SKAMVPLIHHWLIIQGQRSMRGLRMNTLGWFDFKSAWFAPPDP"
     regulatory      7140..7239
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            7270..8253
                     /gene="thiB"
                     /locus_tag="NBI74_003232"
     CDS             7270..8253
                     /gene="thiB"
                     /locus_tag="NBI74_003232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter substrate binding
                     subunit"
                     /protein_id="HCD1933516.1"
                     /translation="MLKKCLPLLLLCTAPVFAKPVLTVYTYDSFAADWGPGPVVKKAF
                     EADCNCELKLVALEDGVSLLNRLRMEGKNSKADVVLGLDNNLLDAASKTGLFAKSGVA
                     TEAVNVPGGWKNDTFVPFDYGYFAFVYDKNKLKNPPQSLKELVESDQNWRVIYEDPRT
                     STPGLGLLLWMQKVYGDNAPQAWQKLAKKTVTVTKGWSEAYGLFLKGESDLVLSYTTS
                     PAYHILEEKKDNYAAANFSEGHYLQVEVAARTAASKQPELAQKFLQFMVSPAFQNAIP
                     TGNWMYPVANVTLPAGFEQLTKPATTLEFTPAEVAAQRQAWISEWQRAVSR"
     gene            8229..9839
                     /gene="thiP"
                     /locus_tag="NBI74_003233"
     CDS             8229..9839
                     /gene="thiP"
                     /locus_tag="NBI74_003233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine/thiamine pyrophosphate ABC transporter
                     permease ThiP"
                     /protein_id="HCD1933517.1"
                     /translation="MATRRQPLIPGWLIPGISAATLVVAVALAAFLALWWNAPQGDWV
                     AVWQDSYLWHVVRFSFWQAFLSALLSVVPAIFLARALYRRRFPGRQGLLRLCAMTLIL
                     PVLVAVFGILSVYGRQGWLASLWQSLGLEWTFSPYGLQGILLAHVFFNLPMASRLLLQ
                     ALENIPGEQRQLAAQLGMRGWHFFRFVEWPWLRRQIPPVAALIFMLCFASFATVLSLG
                     GGPQATTIELAIYQALSYDYDPARAAMLALIQMVCCLGLVLLSQRLSKAIAPGTTLLQ
                     GWRDPDDRLHSRICDTALIVLALLLLLPPLLAVIVDGVNRQLPEVLAQPVLWQALWTS
                     LRIALAAGVLCVVLTMMLLWSSRELRARQKMLAGQALEMSGMLILAMPGIVLATGFFL
                     LLNNTIGLPQSADGIVIFTNALMAIPYALKVLENPMRDITARYSMLCQSLGIEGWSRL
                     KVVELRALKRPLAQALAFACVLSIGDFGVVALFGNDDFRTLPFYLYQQIGSYRSQDGA
                     VTALILLLLCFLLFTVIEKIPGRNVKTD"
     gene            9823..10521
                     /gene="thiQ"
                     /locus_tag="NBI74_003234"
     CDS             9823..10521
                     /gene="thiQ"
                     /locus_tag="NBI74_003234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter ATP-binding protein
                     ThiQ"
                     /protein_id="HCD1933518.1"
                     /translation="MLKLTDITWLYHHLPMRFSLTVERGEQVAILGPSGAGKSTLLNL
                     IAGFLTPASGLLTLDGVDHTTTPPSRRPVSMLFQENNLFSHLTVAQNIGLGLNPGLKL
                     NAAQQKKMHAIAHQMGIDNLMARLPGELSGGQRQRVALARCLVREQPILLLDEPFSAL
                     DPALRQEMLTLVSTSCQQQKMTLLMVSHSVEDAARIATRSVVVADGRIAWQGKTDELL
                     SGKASASALLGIKG"
     gene            complement(10635..11399)
                     /locus_tag="NBI74_003235"
     CDS             complement(10635..11399)
                     /locus_tag="NBI74_003235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001148402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCD1933519.1"
                     /translation="MQALLEHFITQSTVYSLMAVVLVAFLESLALVGLILPGTVLMAG
                     LGALIGSGELSFWHAWLAGIVGCLLGDWISFWLGWRFKKSLHRWSFLKKNKALLDKTE
                     HALHQHSMFTILVGRFVGPTRPLVPMVAGMLDLPVAKFITPNIIGCLLWPPFYFLPGI
                     LAGAAIDIPAGMQSGEFKWLLLVTAVLLWVGGWLCWRLWRSGKATDRLSHYLSRGRLL
                     WLTPLISAIGVVALVVLIRHPLMPVYIDILRKVVGG"
     gene            complement(11583..13070)
                     /locus_tag="NBI74_003236"
     CDS             complement(11583..13070)
                     /locus_tag="NBI74_003236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000350780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HCD1933520.1"
                     /translation="MDILIQALTYINLNAILAIIAATAFGLFVGAIPGLTATMAVALM
                     VPFTFFLDPISALALMIAVGASSIYAGDIPGALLRIPGTPASAAYVADSNELVKQGKV
                     NRVLGLGLVSSVIGGVIGSLILMLAAPVLAKFALNFSSYEYTWLSLLGLTCATLVVGN
                     QPLKGMVTLCLGLLLACIGYDQISGVPRFTFGQISLLQGVNFIPAMIGLFAISGAIEY
                     YRDKISQVSGVVPQTQTGINLFKGMGQVIWSRKLNLGRSSIIGTLIGALPGAGADIAA
                     WISYAISKKFSRQQELYGHGSEEAIVDSSSSNNASLAGSWIPSLVFGIPGDSAAAIII
                     GVLYMKDMNPGPTLFLFQADKLYAVFILFLIANIALLPLATIAVSFIKRIIWIDKAIL
                     YPIILIFSIVGAFAIDNSGASVVVMLVMGVLGYWLQRKEYPVSPIILGMILGPMLEKN
                     LLSSMIKSNGEWLAFVERPVSMALAVCFFLVVALQGRNIYRSFSR"
     gene            complement(13080..13517)
                     /locus_tag="NBI74_003237"
     CDS             complement(13080..13517)
                     /locus_tag="NBI74_003237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001220300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HCD1933521.1"
                     /translation="MRIIPITPLFLLVFAMFIFGWSITQYGGISQYGAGFMPCIISGL
                     LLFLAAIDVAIDIKHRHRNKVSLTFDEVKALLLVITVVLLFVLLLDILGFVICSFLLL
                     FILMKIRGVRLLHSVIVSISASLIIWLIFARVLLVAFPEGILF"
     gene            complement(13566..14519)
                     /locus_tag="NBI74_003238"
     CDS             complement(13566..14519)
                     /locus_tag="NBI74_003238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000831650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HCD1933522.1"
                     /translation="MKRTLMSCAVASLLVSGSALAAWPEKPIQIIVPWGAGGNTDTVA
                     RLVAEGLQKELKVNVNVVNRTGGAGVVGHDAIAKAKADGYTLGIATVEITMMHHQGMT
                     ALTYQNYTPITRLAVNLGGVQVAANSQFENIVQMTDYIKANPGKLKASGSGLNSGWHL
                     NLIGMLDSMGLPPSAVTWVPSEGSSSALMELVSGGIDFTTSSPGEAKSMVDAKMVRNL
                     ATMAEKSAGLYKDLPVFQQATGYKYHLATWNALVVPAGTPEEVSQKLTAAMQAVFAQG
                     KLQEFATKQGFEVYPLYGKEMTTFMADEDARYGKLIAKIKG"
     gene            complement(14574..15497)
                     /locus_tag="NBI74_003239"
     CDS             complement(14574..15497)
                     /locus_tag="NBI74_003239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000848329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HCD1933523.1"
                     /translation="MKTIEGIIPVMLTPFTADNKIDYLGLKRLIDWYIENGADALFAV
                     CQSSEMQYLTLEERVELASFVVEYSQQRVPVIASGHISDDLEQQIQELNAMAATGIDA
                     LVLVTNHLDPHNEGTTVFLRTLDALLMSLPESMALGLYECPAPYRRLLTDEELLYCAQ
                     SGRFQILKDVSCDLPTVTRRVQLTQHTPMTIVNANAAIAWEAMLAGSKGFCGVFANFH
                     PDLYHWLWLEGKQHPEEAKQLAWFLSLSAVSENIGYPKNAKIFHQQRGTFDCVHCRVT
                     ADDVLKKYWGLEVVLKQIADAADFQRQQLKY"
     gene            complement(15523..16755)
                     /locus_tag="NBI74_003240"
     CDS             complement(15523..16755)
                     /locus_tag="NBI74_003240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000285497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HCD1933524.1"
                     /translation="MYRDTRREYIVRLLRNKDECSVSDLANYFSVTKETIRTDLAWLQ
                     KRGVVSRHHGGVSLKKHLMQNALSQQEYVDMSLLIKQQQQGIGYLTSLDDKGRRMVGK
                     VCVLGSFNVDIVAKVYRFPNEGETLVARETTMGPGGKGANQALAAHRAGAQIHFACKI
                     GRDQFNHFARNHIESIGIGSFTFYESELSTTGCAVIYVNDEGENMIAMSPGANHELSD
                     NDIIQLSHFIAESDVFIVQMENNLAATQLALKCAQKMQVTTILNPAPWSSDVATLLPF
                     VDIVTPNETEASAMSGMVIDNISDAVKAAKHIYNAGQCSVIITMGKRGALIYDGQQCA
                     HIPAFSAVAVDTTGAGDAFNGALAAALAKGESLVKAAWYASAFASLAVEKEGAANMPD
                     ISLVDARIQQQNVAIQTF"
BASE COUNT         4299 a         4456 c         4184 g         3910 t
ORIGIN      
        1 gataggtttt gtaacgcgtg cattttatcc ccctttccct cgttgattag atgcaaaaaa
       61 ttattctgaa atatgtcaac cgatgaaaag catcggcggt taagcagaaa ttaatatcgc
      121 ttgttttaac caccgcagca caattagcta attttacgga tgccgaactc acaatggcgt
      181 gacgttttta ttgcttcagg gttgacatcc gtttttgtat ccagtaactc taaaagcata
      241 tcgcattcat ctggagctga tttaatgact cacatcgttc gctttatcgg tctactacta
      301 ctaaacgcat cttctttgcg cggtagacga gtgagcggca tccagcatta agccagcacg
      361 cagtcaaaca aaaacccgcg ccattgcgcg ggttttttta tgcccgaagc gaggcgctca
      421 aaaagagaca aggacccaaa ccatgagcca gcaagtcatt attttcgata ccacattgcg
      481 cgacggtgaa caggcgttac aggcaagctt gagtgtgaaa gaaaaactgc aaattgcgct
      541 ggcccttgag cgtatgggtg ttgacgtgat ggaagtcggt ttccccgtct cttcgccggg
      601 tgattttgaa tcagtgcaga ccatcgctcg ccaggttaaa aacagccgtg tatgcgcgtt
      661 agctcgctgc gtggagaaag atatcgacgt ggcagccgaa tccctgaaag tcgccgaagc
      721 cttccgtatt cataccttta ttgccacttc gccaatgcac atcgccacca agctgcgcag
      781 cacgctggac gaagtgatcg aacgcgctat ctatatggtg aaacgcgccc gtaattacac
      841 cgatgatgtt gaattttctt gcgaagatgc cggacgcacc ccaattgccg atctggcgcg
      901 agtggtcgaa gcggcgatta atgccggtgc caccaccatc aacattccgg acaccgtggg
      961 ctacaccatg ccatttgagt tcgccggaat catcagcggc ctgtatgaac gcgtacccaa
     1021 catcgacaaa gccattattt ccgtacatac ccacgacgat ttgggcctgg cggtcggcaa
     1081 ctcactggcg gcggtacatg ccggagcacg gcaggtagaa ggcgcaatga acgggatcgg
     1141 cgagcgtgcc ggtaactgtt cccttgaaga agtcatcatg gcgatcaaag ttcgtaagga
     1201 tattctcaac gtccacaccg ccatcaatca ccaggagata tggcgcacca gccagttagt
     1261 tagccagatt tgtaatatgc cgatcccggc aaacaaagcc attgttggca gcggcgcatt
     1321 cgcacactcc tccggtatcc accaggatgg tgtactgaaa aaccgcgaaa actacgaaat
     1381 catgacacca gaatctattg gtctgaacca aatccagctg aatctgacct ctcgttcggg
     1441 gcgtgcggcg gtgaaacatc gcatggatga gatgggatat aaagaaagtg aatataattt
     1501 agacaacctg tacgacgctt tcctgaagct ggcggacaaa aaaggccagg tgtttgatta
     1561 cgatctggag gcgctggcct tcatcggtaa gcagcaagaa gagccggagc atttccgtct
     1621 ggattacttc agcgtgcagt caggttctaa cgatattgcc actgccgccg tcaaactggc
     1681 ctgtggcgaa gaagttaaag cagaagccgc taacggcaac ggcccggttg atgccgtcta
     1741 ccaggcgata aaccgcatca ctgactataa cgtcgaactg gtgaaataca gcctgaccgc
     1801 caaaggtcac ggtaaagatg cgctgggtca ggtggatatt gtcgccaact acaacggtcg
     1861 ccgcttccac ggcgtcggcc tggccaccga tattgtcgag tcctccgcca aagccatggt
     1921 gcacgtactg aacaatatct ggcgtgccgc agaagtcgaa aaagagttgc aacgcaaagc
     1981 tcaacacaac gaaaacaaca aggaaaccgt gtgatgtcga agaattacca tattgccgta
     2041 ttgccggggg acggtattgg tccggaagtg atgacccagg cgctgaaagt gctggatgcc
     2101 gtgcgcaacc gatttgcgat gcgtatcacc accagccatt acgatgtagg cggcgcagcc
     2161 attgataatc acgggcagcc gctgccgcct gcgacggttg aaggttgtga gcaagccgat
     2221 gccgtgctgt ttggctcggt aggtggtccg aagtgggaac atttgccgcc agaccagcaa
     2281 ccagaacgcg gcgcactgct gcctttgcgt aaacacttca aattattcag caatctgcgt
     2341 ccggcaaaac tgtatcaggg gctggaagca ttctgtccgc tgcgtgctga cattgccgca
     2401 aacggctttg acatcctgtg tgtgcgcgaa ctgaccggcg gcatctattt cggtcagcca
     2461 aaaggccgcg aaggtagcgg acaatatgaa aaagcgtttg ataccgaggt gtaccaccgt
     2521 tttgagatcg agcgtattgc ccgcatcgca tttgaatctg cccgcaagcg tcgccacaaa
     2581 gtcacctcaa tcgacaaagc caacgtgctg caatcctcta ttttatggcg ggagatcgtt
     2641 aacgagatcg ccacggaata cccggatgtc gaactggcgc atatgtacat cgacaacgcc
     2701 accatgcagc tgattaaaga tccatcacag tttgacgtct tgctgtgctc caacctgttt
     2761 ggcgacattc tgtctgatga gtgcgcaatg atcactggct caatggggat gttgccttcc
     2821 gccagcctga acgagcaagg ttttggtctg tatgaaccgg cgggcggctc ggcacccgat
     2881 atcgcaggta aaaatatcgc caacccgatt gcacaaattc tgtcgctggc gctgctgctg
     2941 cgttacagcc tggatgccga tgatgcggct tccgccattg aacgcgccat taaccgcgca
     3001 ttagaagaag gcattcgcac cggggattta gcccgtggcg ctgccgccgt tagtaccgat
     3061 gaaatgggcg atatcattgc ccgctatgtg gcagaagggg tgtaatcatg gctaagacgt
     3121 tatacgaaaa attattcgac gctcacgtag tgtacgaagc cgaaaacgaa accccgctgt
     3181 tatatatcga ccgccacctg gtgcatgaag tgacctcacc gcaggcgttc gatggtctgc
     3241 gcgcccatgg tcgcccggta cgtcagccgg gcaaaacctt cgccaccatg gatcacaacg
     3301 tctctaccca gacgaaagac attaatgcct gcggtgaaat ggcgcgcatc cagatgcagg
     3361 agctgatcaa aaactgcaaa gaatttggcg tcgagctgta tgacctgaat cacccgtatc
     3421 aggggatcgt ccacgtaatg gggccggaac agggcgtgac cttgccgggg atgaccattg
     3481 tctgcggcga ctcacatacc gccacccacg gcgcgtttgg cgcactggcc tttggtatcg
     3541 gcacttccga agttgaacac gtactggcaa cgcaaaccct gaaacagggc cgggcgaaga
     3601 ccatgaaaat tgaagtccag ggcaaagccg cgccgggcat taccgcaaaa gatatcgtgc
     3661 tggcaattat tggtaaaacc ggcagcgcag gcggtaccgg gcatgtggtg gaattttgcg
     3721 gcgaagcaat ccgtgattta agcatggaag gtcgtatgac cctgtgcaat atggcaatcg
     3781 aaatgggcgc aaaagccggt ctggttgcac cggacgaaac cacctttaac tatgtcaaag
     3841 gccgtctgca tgcgccgaaa ggcaaagatt tcgacgacgc cgttgcctac tggaaaaccc
     3901 tgcaaaccga cgaaggcgca actttcgata ccgttgtcac tctgcaagca gaagagattt
     3961 cgccgcaggt cacctggggc accaatccag gccaggtgat ttctgtgaac gacaatattc
     4021 ccgatccggc ttcgtttgcc gatccggttg aacgcgcgtc ggcagaaaaa gcgctggcct
     4081 atatggggct gaaaccgggt attccgctga cagaagtggc tatcgacaaa gtgtttattg
     4141 gttcctgtac taactcgcgt attgaagatt tacgcgcggc agcggaaatc gccaaagggc
     4201 ggaaagtcgc gccaggcgta caagcgctgg tggttcccgg ctccggtccg gttaaagccc
     4261 aggcggaagc ggaaggtctg gacaaaattt ttatcgaagc tggttttgaa tggcgcttgc
     4321 caggttgttc aatgtgtctg gcgatgaata acgaccgtct gaatccgggc gaacgttgcg
     4381 cctccaccag caaccgtaac tttgaaggcc gccaggggcg cggcgggcgc acgcatctgg
     4441 tcagcccggc aatggctgcc gccgcggctg tgaccggaca tttcgccgac attcgcaaca
     4501 ttaaataagg agcacaccat ggcagagaaa tttatcaaac acacaggcct ggtggttccg
     4561 ttggatgccg ccaatgtcga taccgatgca atcatcccga aacagttttt gcagaaagtg
     4621 acccgtacgg gttttggcgc gcatctgttt aacgactggc gttttctgga tgaaaaaggc
     4681 caacagccaa acccggactt cgtgctgaac ttcccgcagt atcagggcgc ttccattttg
     4741 ctggcacgag aaaacttcgg ctgcggttcc tcgcgtgagc acgcgccgtg ggcattgacc
     4801 gattacggtt ttaaggtggt gattgcgccg agttttgctg acatcttcta cggcaatagc
     4861 tttaacaacc agctgctgcc ggtgaaatta agcgatgcag aagtggatga gctgtttgcg
     4921 ctggtgaaag ccaatccagg gattcatttt gatgtagacc tggaggcgca agaggtgaaa
     4981 gcgggagaga aaacttatcg ctttaccatc gatgccttcc gccgccactg catgatgaac
     5041 ggtctggaca gcatcggcct gactctgcaa cacgacgatg ctattgcgtc ttatgaagag
     5101 aagcaaccgg cgtttatgcg ttagtttgta caccgtccgg cactcaatcg ccaaaagaaa
     5161 aaagccagca gataatcact gctggcgtgg cgattaacac caatactcag tcacacatga
     5221 tgtccgcaaa tcagtagtca gaatgactcc cctcccattg catcagttct tcaagcatgg
     5281 ttaatcgttg tgggacgctt aaccaacgca accagcacaa cttcgctgtc gcggtaaaat
     5341 agtgccgata aaactgacgc atggggcacc tccttgcttc atcgttagga attattgact
     5401 taatataggg aaaataaaat tgctgtattt tgcgcaggag ttcccctttt atgccatctg
     5461 ctcgtctgca acaacagttc atccgcctgt ggcaatgctg cgagggtaaa tctcaggaga
     5521 ccacgctcaa cgaactggca gcgttactga gttgctcgcg tcgtcatatg cgcaccctgc
     5581 tcaacaccat gcaggatcgc ggctggctga cgtgggaagc ggaagtcggg cgcggtaaac
     5641 gctcgcgtct gacattcctc tataccgggc tggcgcttca gcaacagcga gcggaagacc
     5701 tgctggagca ggatcgtatc gaccaactag tgcagttggt tggcgacaaa gcgactgtgc
     5761 ggcaaatgct ggtttctcat ctgggccgca gcttccgcca ggggcggcac atcctgcgcg
     5821 tgctctacta tcgtccgttg cgtaatctgc tacctggcag cgcattgcgc cgttctgaaa
     5881 cccatatcgc ccggcaaatc ttcagttcgc taacgcgcat aaatgaggaa aatggggaac
     5941 tggaagcaga catcgcacac cactggcagc aaatatcacc gcttcactgg cgtttctttt
     6001 tgcgtcctgg agtccatttt caccatggtc gagaactgga aatggacgat gtgatcgcct
     6061 ctttaaaacg aatcaataca cttccgctct attcgcatat tgctgacatt gtgtcgccga
     6121 cgccctggac gctggatatc catctcacgc aaccggaccg ctggttgccg ttactgctgg
     6181 ggcaagttcc ggcaatgatc ctgccgcgcg aatgggaaac cctcagtaac tttgccagcc
     6241 atcccatcgg caccggtccg tatgcggtga tccgcaacag caccaatcaa ctgaaaattc
     6301 aggcattcga tgacttcttc ggttaccggg cattaatcga cgaagttaac gtctgggttc
     6361 tgccggaaat tgccgacgag ccagccggag ggctgatgct gaaagggccg cagggcgagg
     6421 aacaagagat tgaaagccgc ctggaggaag gttgctatta tttactgttc gacaaccgca
     6481 cccaccgcgg ggcaaatcag caagtcaggg actgggtaag ctatgtgctt tctccgacga
     6541 atctggtcta tttcgccgag gaacaatacc agcaactctg gttcccggct tatggactgc
     6601 tcccccgctg gcaccatgcc cgcccgacac attgcgaaaa acccgccggg ctggaacatc
     6661 tcaccctgac cttttatcag gatcatattg aacatcgggt gattgccggg atcatgcagc
     6721 agattctggc gagtcaccag gtcacgctgg aaatcaaaga gatcagctac gatcagtggc
     6781 atgaaggaga gatcgagagc gatatctggc ttaacagcgc caactttacg ctgccgctgg
     6841 atttttcgct gttcgcgcac ttgtgcgagg tgccgctact ccaacactgt attccgatcg
     6901 actggcaagc cgacgccgcc cgctggcgca atggcgaaat gaacctggcg aactggtgcc
     6961 agcaactggt cgctagcaaa gcaatggtgc cacttatcca ccactggctg atcattcagg
     7021 ggcaacgcag tatgcgcggc ctgcgcatga acaccctcgg ctggttcgat tttaaatcag
     7081 cgtggtttgc accaccggat ccatgattgc tggttgataa caaaatcact acactaacgc
     7141 cgttctcaac ggggtgccac gcagccgcgt gcgctgagaa aatacccgtc gaacctgatc
     7201 cggataacgc cggcgaaggg atttgaggct ccttctcaag tcctttgcca ctcttttgtg
     7261 aggtgcaaag tgttaaaaaa atgtcttccc ctgctgttgc tgtgcacagc gcctgttttc
     7321 gctaaacctg ttctgactgt ttatacctac gattcctttg ccgctgactg gggacctggt
     7381 ccggtagtca aaaaagcctt tgaagcagac tgtaattgcg aactgaaact ggtggcgctg
     7441 gaagatggcg tttcgctgct caaccgtcta cggatggaag gcaaaaacag caaagccgat
     7501 gtggtgctgg ggctggataa caacctgctg gacgccgcca gcaaaaccgg gttgtttgcc
     7561 aaaagcggcg tcgcgacaga ggctgtaaac gttcccggcg gctggaaaaa tgacaccttt
     7621 gtgccgtttg actatggcta tttcgccttc gtctatgaca agaacaaact gaaaaatccg
     7681 ccgcaaagcc tgaaagaact ggttgagagc gatcaaaact ggcgggtgat ttatgaagat
     7741 ccgcgtacca gtacaccagg tctgggtctg ttgctatgga tgcaaaaagt ctatggcgat
     7801 aacgccccgc aagcctggca gaaactggcg aagaaaactg tcacggtcac caaaggctgg
     7861 agcgaagcct acggcctgtt tttaaaaggc gaaagcgatc tggtactgag ttacaccacc
     7921 tctccggctt atcacattct cgaagagaag aaagataact acgccgccgc gaacttcagc
     7981 gaaggtcact atctgcaggt ggaagttgcc gcccgcaccg ctgccagcaa gcagccagag
     8041 ctggcgcaaa aattcctcca gtttatggtt tctccggctt ttcagaatgc gatcccaacc
     8101 ggcaactgga tgtatccggt agcaaacgtc actctgcctg caggatttga acaattaacc
     8161 aaaccagcca ccacgctaga gttcacgccc gccgaagtgg cggcacaacg tcaggcatgg
     8221 attagcgaat ggcaacgcgc cgtcagccgt taattcccgg ctggttaatt ccaggaataa
     8281 gcgccgccac actggtggta gcggttgcgc tggcggcgtt tctcgccctg tggtggaacg
     8341 cgccgcaggg tgactgggtg gcagtctggc aggacagcta tctgtggcat gtggtgcgct
     8401 tctccttctg gcaggcgttt ctctcggcgc tgctctctgt cgtacccgca atattcctcg
     8461 cccgcgcgct ctatcgcagg cgctttcctg ggcgacaggg gctgttgcgt ctgtgcgcaa
     8521 tgaccttgat cctcccggtg ctggtcgccg ttttcggcat tcttagcgtc tatggtcgcc
     8581 agggctggct ggcctcgctc tggcaatcac ttggtctgga gtggaccttt tcgccctacg
     8641 gcctgcaagg cattttgctg gcccatgtgt tttttaatct gccgatggct agccgtttat
     8701 tactccaggc actggaaaac attcccggcg aacaacgtca gcttgccgca caacttggga
     8761 tgcgcggctg gcacttcttc cgcttcgtcg aatggccgtg gttacggcga caaatcccgc
     8821 cggttgctgc gcttatcttt atgctctgtt tcgccagctt cgccaccgtg ctatcgctgg
     8881 gcggcggtcc gcaggcgacc actatcgagc tggcaatcta tcaggcgctg agttacgact
     8941 acgatcctgc ccgcgcggca atgctggcgc tgatccagat ggtgtgctgc ctcgggctgg
     9001 tgctgttgag tcagcgattg agtaaggcca ttgcgcccgg taccacactg ctgcaaggct
     9061 ggcgcgaccc ggacgatcgt ctgcatagtc gcatttgcga cacggcgtta attgtgctgg
     9121 cgctgctgct gttgctgcca ccgttactgg cggtgatcgt cgatggggta aatcgccagt
     9181 tgccggaagt gctggcacaa ccggtgctgt ggcaggcgct gtggacctcg ttgcgtattg
     9241 cgctggcggc aggcgtattg tgcgtagtgc tgaccatgat gctgctatgg agcagtcgcg
     9301 aacttcgggc acggcagaaa atgctggcgg gtcaggcgct ggagatgagc ggcatgttga
     9361 tcctcgccat gccggggatt gtactggcta cgggcttctt tttactgctc aacaacacta
     9421 tcggcctgcc acaatctgct gacggcattg tgattttcac caatgcgtta atggcaatcc
     9481 cttatgcgct gaaagtactg gaaaacccga tgcgcgatat caccgcccgc tacagcatgt
     9541 tgtgtcagtc gctgggcatt gaaggctggt cacgcttaaa agtggtggag ctgcgcgccc
     9601 tgaaacgtcc actggcgcag gcgctggcct ttgcttgtgt gctgtcgatt ggtgattttg
     9661 gcgtggtggc attgttcggt aacgatgatt tccgtaccct gccgttttat ctctatcagc
     9721 aaattggctc ctatcgcagc caggacggcg cggtcaccgc gttaattctg ctgctgctct
     9781 gttttctgct gtttaccgtg attgaaaaaa taccggggcg aaatgttaaa actgactgat
     9841 atcacctggc tttaccacca tttgccgatg cgttttagct taacggtgga acgcggcgag
     9901 caggtggcga tcctcgggcc aagcggcgcg ggtaaaagca ccctgctaaa tttgatcgcc
     9961 ggttttctga cgccagccag cggtttgctg actctcgacg gcgtagatca caccacaacg
    10021 ccaccgtcac gtcgtccggt gtcaatgctg tttcaggaga acaacctgtt cagccacctg
    10081 acggtcgcgc aaaacatcgg gctggggcta aatccggggt tgaaactaaa tgcggcgcag
    10141 cagaagaaaa tgcacgcgat tgcccaccag atggggattg ataatttaat ggcgcggtta
    10201 cctggcgagc tttccggcgg tcagcgacag cgcgtggcgt tagcgcgttg tctggtacgc
    10261 gaacagccca ttttattgct cgatgaaccg ttttccgcgc tcgatccggc gctacgtcag
    10321 gagatgctga cgctggtgag cacgagctgc caacagcaaa aaatgacgct attgatggtg
    10381 tcgcacagtg tggaagatgc ggcgcggatc gccacgcgct cggtggtggt cgccgacggg
    10441 cgtatcgcct ggcagggtaa gaccgatgag ttgttgagcg gtaaggcgag tgcttcggcg
    10501 ctgttgggaa ttaaaggtta atatgcactg tcggatgcgg cgtgaacgcc ttatccgacc
    10561 tacgggtagt gcgttttgca ggcctgataa gacgcggcaa gcgtcgcatc aggcaacccg
    10621 cacaaggcta tcccctaacc cccaaccact ttacgcaaaa tatcgatata caccggcatc
    10681 aacgggtggc gaattaacac caccagcgcc accacgccga tggcagaaat caacggcgtc
    10741 agccacaaca aacgaccgcg ggacaaataa tgactcaaac ggtcagtcgc tttaccgctg
    10801 cgccataacc gccagcacag ccagccacca acccacaaca ataccgccgt caccagcagc
    10861 aaccatttaa actcaccgct ctgcatcccg gcaggaatat cgatcgccgc acccgccaga
    10921 atccccggca ggaagtaaaa cggtggccat aacaggcagc cgataatatt tggcgtaata
    10981 aatttcgcca ccggcagatc cagcatccct gccaccattg gcaccagcgg acgcgtcggg
    11041 ccgacaaaac gaccgaccag aatggtgaac atgctgtgct gatgcagcgc atgttcggtt
    11101 ttatcgagta atgctttgtt cttcttcaga aatgaccagc gatgcaacga ctttttaaaa
    11161 cgccagccca gccagaagga gatccagtcg cccagcaggc aaccgacgat ccctgccagc
    11221 caggcgtgcc agaaacttaa ctcgccgctg ccaatcaacg ctcccagccc cgccatcagc
    11281 accgtaccgg gcagaatcaa accgaccagc gccagcgact ccagaaaggc caccaacacc
    11341 accgccatca atgaatacac ggtggattgg gtaataaagt gttccagcaa tgcttgcata
    11401 atgtgcccgg ttgatgaatt cgtcgtggat tgtgcaaagt ggtggtattc aggtcaatct
    11461 attctttgta tgaatatatt cctgcaacgc gaatcattta gagtataaat ttagcagtca
    11521 cgcaggattt tacttagggg aaggcagacc atccacaact tggatggtct gaaaacaacg
    11581 gattaacggc taaatgaacg gtagatattg cgtccttgca acgcgacaac caaaaagaaa
    11641 cagacagcca gtgccataga taccgggcgc tcaacaaacg ccagccattc gccattagac
    11701 ttaatcattg aagataataa gtttttttcc aacattggcc caagaatcat ccccagaata
    11761 atgggcgaaa ccgggtattc cttacgttgt aaccagtatc caagaacccc catgaccaac
    11821 atgacaacga cagaggcacc cgagttatcg atagcaaatg caccgacaat actgaaaatc
    11881 agaataatcg ggtacagaat cgctttatca atccagatga tgcgtttgat gaaactgacc
    11941 gcaattgtgg ctaacggtag taatgcaata ttggcgatca gaaacaagat aaatacggca
    12001 taaagtttat ctgcctggaa cagaaataat gtcggccctg gattcatatc cttcatatag
    12061 agcacgccga tgataattgc agcggcagaa tcgcccggaa taccgaaaac taatgaaggg
    12121 atccaactcc ccgccagact ggcgttgtta ctgctcgagg aatcaacaat tgcctcctca
    12181 gatccatgcc catataactc ctgctggcga gagaattttt ttgaaatggc gtaacttatc
    12241 cacgcagcaa tatccgctcc agcgccgggt aatgctccga taagcgtacc gataatacta
    12301 cttcggccaa gatttaattt tcgtgaccaa atcacctgcc ccattccctt gaataaatta
    12361 atccccgttt gcgtttgtgg cacaacacca cttacctggc taattttgtc acgataatat
    12421 tcaattgcgc cggatatcgc gaacagacca atcatcgccg ggataaagtt caccccttgt
    12481 aacaggctga tttgaccaaa cgtaaaacgt ggaaccccgg atatttgatc ataaccgata
    12541 cacgccagta ataatcccaa acataaggtc accatgcctt tcagtggttg attacccaca
    12601 accagagtcg cgcaggttaa gcctaaaaga gaaagccatg tgtattcata agaactaaaa
    12661 ttaagggcaa atttcgccag tacaggcgca gccaacatca aaataagtga tccaatcacg
    12721 ccaccaatga ccgaactcac cagcccaagt cccaatacgc gattcacctt cccttgcttg
    12781 accagttcat ttgaatcagc aacataagcc gccgaagcag gtgtccctgg tatacgcaaa
    12841 agtgcgccgg ggatatctcc ggcataaatg gatgacgcac caacggcaat cattaatgcc
    12901 agggcggaaa taggatcaag aaagaaggta aaaggcacca ttagtgcaac cgccatcgtt
    12961 gcggtcaatc cagggatggc ccccacaaat aacccaaagg ccgtagcagc aataatggcc
    13021 agaatggcat tcaaattaat atacgttagt gcctgaatca aaatatccat attgcattct
    13081 taaaacaaga ttccttcagg aaaggcgacc aacaacactc tggcaaaaat gagccagata
    13141 ataagagatg ccgatatcga aacgataacg gaatgcaaaa gtctgacacc tcgtattttc
    13201 attaaaataa aaagcaacaa aaatgagcaa atcacaaagc cgagaatatc taacagtaat
    13261 acaaacagta gtaccactgt aataaccagt aaaagtgctt tcacttcatc aaaagttaaa
    13321 gaaactttgt tacggtgccg gtgtttaata tctatcgcta catcaatagc cgctaaaaac
    13381 aacaacaatc cactgatgat gcaaggcata aatccagcgc catactgcga tatgcctcca
    13441 tattgcgtta tgctccagcc aaaaataaac atggcaaaaa ccagcagaaa taacggcgtt
    13501 atcggtatta tgcgcataat gctatccctg taatttggcg gcgatatatt caccgccctg
    13561 tctgattaac ctttgatttt ggctattaat tttccgtatc tggcatcttc atctgccata
    13621 aatgtcgtca tctctttgcc atacagcggg taaacttcga aaccctgttt ggtagcgaat
    13681 tcttgtaatt tcccctgcgc aaacactgcc tgcatagcgg cagttaattt ttgtgagacc
    13741 tcttcaggag tacccgccgg aaccactaag gcattccatg tcgccaaatg atatttatag
    13801 ccagttgctt gttggaatac tggaagatct ttataaagcc ctgctgattt ttctgccatg
    13861 gtcgctaaat tacgcaccat tttggcatcg accatacttt tcgcttcgcc tggtgatgac
    13921 gttgtaaaat ctataccacc ggaaaccagt tccatcagtg cagaactgga accttcagaa
    13981 ggcacccagg tcacggcaga aggcggaagc cccatactgt cgagcatacc tatcaagttc
    14041 agatgccagc cggaatttaa cccgctaccc gatgctttta acttacctgg attcgccttg
    14101 atgtagtcgg tcatctggac gatattttcg aactgcgaat ttgctgcgac ttgcacgcct
    14161 cccagattca ctgccagccg tgtaattggc gtgtagttct gatacgttaa cgccgtcatc
    14221 ccctgatggt gcatcatggt gatttctacc gtcgcaattc ccagggtata gccatccgct
    14281 ttagctttcg caatggcatc atggccaacc accccagcac ctcccgtgcg attcaccacg
    14341 ttgacattca ctttcaactc tttttgcagc ccttctgcca ccaggcgtgc aaccgtatcg
    14401 gtatttcctc ccgcgcccca ggggacaatg atttggattg gtttttctgg ccatgcagcc
    14461 agcgcggaac ctgaaaccag cagtgaagca acggcacaac tcatcagggt tcgtttcata
    14521 tttgtagact cctgtcttaa ttatttttta ataacagcaa gtgctgtcgg atattaatat
    14581 ttgagttgtt gacgctggaa atctgcggca tcagcaattt gttttagcac cacctccagc
    14641 ccccaatatt ttttcagcac atcgtcagcc gttacccggc aatgaacaca gtcaaatgtt
    14701 cctcgctgct gatgaaatat tttggcattt ttcggatagc caatattttc actgacggca
    14761 ctcagcgata aaaaccaggc gagttgtttt gcctcttcag ggtgttgttt tccttcaagc
    14821 caaagccagt gatacaagtc aggatgaaag ttggcaaaga cgccacaaaa acctttcgaa
    14881 cctgcaagca tcgcctccca ggcaatggca gcattagcat taacaatcgt cattggcgtg
    14941 tgctgagtta attgcacccg tcgcgtcact gtaggtagat cacaactgac gtcttttaag
    15001 atttgaaaac gcccgctttg tgcgcagtaa agcagttcct catcggttaa taagcggcga
    15061 tacggggccg ggcattcata cagacccagc gccatacttt cgggtaatga catcaatagc
    15121 gcatcaagcg tacggaggaa taccgtggta ccttcgttat gtggatcaag atgattcgtc
    15181 actaacacca gcgcatcaat gcccgttgcc gccatagcat tgagctcctg aatttgctgt
    15241 tccagatcat cagaaatatg cccggacgca ataaccggta ctcgttgttg gctatattca
    15301 accacaaagc tcgccagctc cacgcgctct tctaaagtta aatactgcat ttcactggac
    15361 tgacacacgg caaataacgc gtcggcaccg ttttcgatat accagtcaat tagccgttta
    15421 agacccaggt aatcaatttt attatccgcc gtaaaaggcg ttagcattac cggaataatc
    15481 ccttcaattg ttttcatatt tcatccttcg ttcagtgaaa catcagaaag tctgaatggc
    15541 aacattctgc tgctgaattc tggcatccac cagagagata tcaggcatat tggctgcccc
    15601 ttctttttct actgccagcg aagcgaaagc gctggcatac caggctgctt taacaagtga
    15661 ttctcccttc gccagtgcgg cggcgagcgc gccattaaac gcatcacccg cacctgttgt
    15721 atctacagcg actgccgaaa atgccggaat atgcgcacat tgttgcccgt cataaattaa
    15781 tgcacctcgt tttcccatcg taataattac tgaacattgc ccggcattat aaatatgctt
    15841 cgcagccttc acagcatcag aaatattgtc aattaccata cctgacatcg ccgatgcttc
    15901 ggtttcattt ggcgtcacaa tatcaacaaa aggtaataat gtggcgacat cactggacca
    15961 cggtgcagga tttaaaatcg ttgtcacctg cattttttga gcacatttta atgccagttg
    16021 agtagcggca agattatttt ccatctgtac aatgaataca tcggactcgg ctataaagtg
    16081 agaaagttga ataatatcgt tatcacttaa ttcatgattt gcccctggag acatagcaat
    16141 catgttttca ccttcatcat ttacataaat gaccgcacaa ccggtggtag atagttcaga
    16201 ctcataaaaa gtaaatgaac caataccaat ggactcaata tgattgcggg caaaatgatt
    16261 aaactgatcg cgaccaatct tacaggcgaa gtgtatttgc gcacctgccc gatgagcggc
    16321 tagtgcctga ttcgcgcctt taccccccgg ccccatggta gtttccctgg ccactaacgt
    16381 ttcgccttca ttcgggaagc gatacacttt cgccacaata tcgacgttaa atgatcctaa
    16441 cacacaaact ttacctacca ttcggcgtcc tttatcatca agcgaggtta aatagccaat
    16501 accctgctgt tgttgcttga taagcaaact catatcgaca tactcttgtt gcgaaagcgc
    16561 attttgcatt aaatgttttt tcaatgagac gcctccatga tgacgactca ctacaccacg
    16621 cttttgcaac cacgctaaat ctgtccgaat cgtctctttg gtaacagaaa aatagttagc
    16681 taaatcgcta acactgcact catccttgtt gcgaagtaac cggacaatat attcacgacg
    16741 ggtatcccga tacataagtc ttcctttttg attttattat cactaaaaaa gaaattgtta
    16801 tcgggatttt tatcaagaaa aaaacaggca agcaaaaaca aacaaaaat
//
LOCUS       DAHHTG010000097        16580 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.097, whole genome shotgun sequence.
ACCESSION   DAHHTG010000097 DAHHTG010000000
VERSION     DAHHTG010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16580)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16580)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16580
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.097"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            complement(24..3050)
                     /locus_tag="NBI74_003241"
     CDS             complement(24..3050)
                     /locus_tag="NBI74_003241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001565308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HCD1933525.1"
                     /translation="MTVKISGVLKDGTGKPVQNCTIVLKARRTSSTVVVNTVASENPD
                     EAGRYSMDVEHGQYSVTLLVEGFPPSHAGTITVYEGSRPGTLNDFLGAMTEDDVRPEA
                     LRRFEQMVEEVSRNASAVAQNTAAAKKSASDASASASEAATHATDAAASARAASTSAG
                     QAASSAQSASSSAGTASTKAREAAKSAAAAESSKSAAATSASAAKTSETNAAASQKSA
                     ATSASTATMKASEAATSARGAAASKEAAKSSETNASSSASSAASSATAAGNSAKAAKT
                     SETNAKSSETAAGQSASAAAGSKTAAALSASAASTSAGQASASATAAGKSAESAASSA
                     STATTKAGKATEQATAAARSASAAKTSETNAKTSADNAASSKAAAASSASSAASSASS
                     ASASKDEATRQASAAKGSATTATTKASEAAGSATAASQSKVAAESAATRAETAAKRAE
                     DIASAVALEDASTTKKGIVQLSSATNSTSEKLAATPKAVKTVKDSSVQKTGDTMGGQL
                     KISTINALRIFNQAFGLIFRRSEDHLHLIPTNEGEGENGDIGSLRPFSINLRSGLVSI
                     GNGLKVGGSVTGNLTGNADTATKIKTARKIGGVAFDGSADINLPGVNATGNQNTTGNA
                     ATATKLQAARTINGVSFDGSANITLTPSNIGALALTGGTLSGGLTAAGEVISRSANGL
                     RIAYGNYGFFIRNDGSNTYFMLTDSGNSLGTHNSLRPFIISNHTGNVTIATKLNASGG
                     ITGSLSGNASTATKLQTARTINGVKFDGSANIEAFPPGVPLPWPSDTPPAGYAIMQGQ
                     TFDKAAYPKLAIAYPSGVIPDMRGWTIKGKPASGRAVLSQEQDGIKSHTHSASASSTD
                     LGTKTTSSFDYGTKSTNNTGAHTHSLSGSTGSAGVHTHGNGIRWPGGGGSALAFYDGG
                     GFTYVQNSQYQVSPETSSYRSYYQRIQTQSAGAHTHSLSGTAASSGAHAHTVGIGAHT
                     HSVAIGSHGHTITVNAAGNAENTVKNIAFNYIVRLA"
     gene            complement(3202..3801)
                     /locus_tag="NBI74_003242"
     CDS             complement(3202..3801)
                     /locus_tag="NBI74_003242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family protein"
                     /protein_id="HCD1933526.1"
                     /translation="MRKVCAAILSAAICLSVSGAPAWASEHQPTLSAGYLHARTNAPG
                     SDNLNGINVKYRYEFTDALGLITSFSYANAEDEQKTHYSDTRWHEDSVRNRWFSVMAG
                     PSVRVNEWFSAYAMVGVAYSRVSTFSGDYLRVTDNKGKTHDVLTGSDDGRHSNTSLAW
                     GAGVQFNPTESVTIDLAYEGSGSGDWRTDAFIVGIGYRF"
     gene            complement(3869..7561)
                     /locus_tag="NBI74_003243"
     CDS             complement(3869..7561)
                     /locus_tag="NBI74_003243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000514742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1983 domain-containing protein"
                     /protein_id="HCD1933527.1"
                     /translation="MGKGGGKAHTPREAKDNLKSTQMMSVIDAIGEGPIEGPVKGLQS
                     ILVNKTPLTDTDGNPVIHGVTAVWRAGEQEQTPPEGFESSGAETGLGVEVTKAKPVTR
                     TITSANIDRLRVTFGVQSLVQTTSKGDRNPSSVRILIQLQRNGRWVTEKDVTINGKTT
                     SQFLASVILDNLPPRPFNIRMVRETADSTTDQLQNKTLWSSYTEIIDVKQCYPNTAIV
                     GLQVDAEQFGGQQMTVNYHIRGRIIQVPSNYDPEKRTYSGIWDGSLKPAYSNNPAWCL
                     WDMLTHPRYGMGKRLGAADVDKWALYAIGQYCDQTVPDGFGGTEPRMTFNAYLAQQRK
                     AWDVLSDFCSAMRCMPVWNGQTLTFVQDRPSDVVWPYTNSDVVVDDNGVGFRYSFSAL
                     KDRHTAVEVNYTDPQNGWQTSTELVEDPEAILRYGRNLLKMDAFGCTSRGQAHRAGLW
                     VIKTELLETQTVDFTLGSQGLRHTPGDIIEICDNDYAGTMTGGRVLSIDAASRTLTLD
                     REVTLPETGTATVNLINGSGKPASVAITAHPAPDRIQVSTLPDGVETYGVWGLSLPSL
                     RRRLFRCVSIRENTDGTFAITAVQHVPEKEAIVDNGARFEPQSGTLNSVIPPAVQHLT
                     VEVSAADGQYLAQAKWDTPRVVKGVRFSLRLTSGKGTDARLVTTAITADTEHRFSGLP
                     LGEYTLTVRAINSYGQQGEPATTTFRIAAPAAPSRIELTPGYFQITAVPRLAVYDPTV
                     QFEFWFSEKRITNTAQVEKSARYLGTGSQWTVQGSRIKPGTDFWFYVRSVNLVGKSAF
                     VEASGQPSNDGEGYLEIFRGLIDETLLGQALKERIDASALRTEVTQLEEDIRQRMDTD
                     IAEVTRKIGKAENSLTQLVAKKNEDQTLAIAQVSQKVDRVSSEISQTVSQGQSENARQ
                     IAQVRQYVDKKGSEITSTTDKKLGDQAVTIQQIQRVQSDTRNELNAMYMLKVQKTKNG
                     IPYVAGIGAGIEDVDGQTLSSILLQADRIAMITPENGNTTPLFVAQGNQLFMNDVFLK
                     RLFAVSITSSGNPPTFSLTPDGRLTARNADISGAITANTGTLNNVTINENCVIRGKLS
                     ANQIEGDLVKTVGKAFPRDSRAPERWPSGTITVRVYDDQPFNRQIVIPAVAFSGARHE
                     RENSDTYSSCRLIVRKNGAEIYNRTALDNTLIYSGVIDMPAGRGHMTLEFSVSAWWVN
                     GWYPTASISDLLVVVMKKATAGITIS"
     gene            complement(7905..8537)
                     /locus_tag="NBI74_003244"
     CDS             complement(7905..8537)
                     /locus_tag="NBI74_003244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001188718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HCD1933528.1"
                     /translation="MCLHGDLQRFGRRLSLYVNTAAEAIRALSMQMPGFRRQMNEGWY
                     QIRIAGDDTAPEAVYARLHEQLGEGTVIHIVPRLAGAGKGGLQIVLGAAAIVGSFFTA
                     GASMALWGTALSAGGFSATTMLFSLGASMILGGVAQMLAPKAKTPDYRATDNGRQNTY
                     FSSLDNMIAQGNPMPVPYGEMLVGSRRISQDISTRDEGGGGKVVVIGRQG"
     gene            complement(8483..9226)
                     /locus_tag="NBI74_003245"
     CDS             complement(8483..9226)
                     /locus_tag="NBI74_003245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCD1933529.1"
                     /translation="MTQTESAILAHARRCAPAESCGFVISTPEGEWYIPCVNISAEPE
                     AYFRIAPEDWLRAEMQGEIVALVHSHPGGLPWLSEADRRLQIKSALPWWLVCRGDIHK
                     FRCVPHLTGRRFEHGVTDCYTLFRDAYHLAGTEMPDFHREDDWWRNGQNLYLDNMAVT
                     GFYRVPLSSAQAGDILLCCFGASVPNHAAIYCGNGELLHHLPEQLSKRERYSEKWQRR
                     THSVWRHRHWHASAFTGIYNDLAAASACM"
     gene            complement(9237..9935)
                     /locus_tag="NBI74_003246"
     CDS             complement(9237..9935)
                     /locus_tag="NBI74_003246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HCD1933530.1"
                     /translation="MQDIHEESLNESVKSEQSPRVVLWEIDLTVQGGERYFFCNELNE
                     KGEPVTWQGREYQAYPIEGSGFEMSGKGSSARPSLTVSNLFGLVTGMAEDLQSLVGAT
                     VVRRRVYARFLDAVNFVAGNPEADPEQELTDRWVVEQMSALTAMTASFVLATPTETDG
                     ALFPGRIMLANTCMWTYRSDECGYTGGAVADEFDKPTTDIRKDRCSKCMRGCELRRNV
                     GNFGGFLSINKLSQ"
     gene            complement(9935..10264)
                     /locus_tag="NBI74_003247"
     CDS             complement(9935..10264)
                     /locus_tag="NBI74_003247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCD1933531.1"
                     /translation="MKTFRWKVKPDMEVNSQPSVREVRFGDGYSQRMAAGLNADLKTY
                     RVTLSVTREEARHLEAFLAEHGGWKAFLWKPPYAYRQIKVICAGWSARVGMLRVEFSA
                     EFKQVVN"
     gene            complement(10261..12822)
                     /locus_tag="NBI74_003248"
     CDS             complement(10261..12822)
                     /locus_tag="NBI74_003248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HCD1933532.1"
                     /translation="MSQPVGDLVIDLSLDAVRFDEQMSRVRRHFSGLDTDARKTATVV
                     EQGLSHQALAAQKAGISVGQYKAAMRTLPAQFTDIATQLAGGQNPWLILLQQGGQVKD
                     SFGGMIPMFRGLAGAITLPMVGVTSLAVATGALVYAWYQGDSTLSAFNKTLVLSGNQS
                     GLTADRMLTLSRAGQAAGLTFNQASESLAAMVNAGVRGGEQFDAINQSVARFASASGV
                     EVDKVAEAFGKLTTDPTSGLIAMAKQFHNVTAEQIAYVAQLQRSGDETGALQAANEAA
                     TKGFDDQTRRLKENMGTLETWADRTARAFKSMWDAVLDIGRPDTAQEMLIKAEAAFKK
                     ADDIWNLRKDDYFVNDEARARYWDDREKARLALEAARKKAEQQTQQDKNAQQQSDTEA
                     SRLKYTEEAQKAYERLQTPLEKYTARQEELNKALKDGKILQADYNTLMAAAKKDYEAT
                     LKKPKQSGVKVSAGDRQEDSAHAALLTLQAELRTLEKHAGANEKISQQRRDLWKAESQ
                     FAVLEEAAQRRQLSAQEKSLLAHKDETLEYKRQLAALGDKVTYQERLNALAQQADKFA
                     QQQRAKRAAIDAKSRGLTDRQAEREATEQRLKEQYGDNPLALNNVMSEQKKTWAAEDL
                     LRGNWMAGLRSGWSEWEESATDSMSQVKSAATQTFDGIAQNMAAMLTGSEQNWRSFTR
                     SVLSMMTEILLKQAMVGIVGSIGSAIGGAVGGGASASGGTAIQAAAAKFHFATGGFTG
                     TGGKYEPAGIVHRGEFVFTKEATSRIGVGNLYRLMRGYAEGGYVGAAGSPAQMRRAEG
                     INFNQNNHVVIQNDGINGQAGPQLMKAVYEMARKGAQDELRLQLRDGGLLSGSGR"
     gene            complement(12803..13216)
                     /locus_tag="NBI74_003249"
     CDS             complement(12803..13216)
                     /locus_tag="NBI74_003249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HCD1933533.1"
                     /translation="MGRPDWRAMLAGMTSTEYADWHRFYRTHYFQDTQLDMHFSGLTY
                     AVLSLFFCDPDMHPSDFSLLVPRHEEEQVERPDEDKMLMQKAAGLAGGVRFGGDGGGD
                     ILSSADVADVMVDDAALMMASAGIPGGVRYVPAGW"
     gene            complement(13243..13674)
                     /locus_tag="NBI74_003250"
     CDS             complement(13243..13674)
                     /locus_tag="NBI74_003250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HCD1933534.1"
                     /translation="MFLKTEQFEYNGVSVTLSELSALQRIEHLALLKRRAEEAEASGN
                     LQVSVEDLVRTGAFLVAMSLWHNHPQKTQSPSMNEAVMKIEQEVLTTWPADAIARAED
                     VVLRLSGMIEAVRPDTDITEVAKNNTLTDDDFSAGKSSTVS"
     gene            complement(13693..14442)
                     /locus_tag="NBI74_003251"
     CDS             complement(13693..14442)
                     /locus_tag="NBI74_003251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000235048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCD1933535.1"
                     /translation="MATPNPLEPVKGAGTTLWVYNGKGDAYANPLSDADWQRLAKVKD
                     LTPGEMTAESYDDNYLDDEDADWTATGQGQKSAGDTSFTLAWKPGEEGQKGLIGWFES
                     GDVRAYKIRFPNGTVDVFRGWVSSIGKAVTAKEVITRTVKVTNVGKPSVAEERSKITP
                     VSAIKVTPTSGTVAKGKTTTLTVTVEPENATDKTFRAISADPSKATISVKDMTITVTG
                     VKDGKVSIPVISGNGQFAAVAEITVNNVPGS"
     gene            complement(14450..14845)
                     /locus_tag="NBI74_003252"
     CDS             complement(14450..14845)
                     /locus_tag="NBI74_003252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCD1933536.1"
                     /translation="MKHTDIRAAVLDALEQHEHGATLFDGRPVVFDEEDFPAIAVYLT
                     DAEYTGEELDADTWRATLHIEVFLPAQVPDSELDQWMESRIYPAMTAIPALAGLITTM
                     VTQGYEYRRDDDMALWSSADLTYSITYEM"
     gene            complement(14842..15375)
                     /locus_tag="NBI74_003253"
     CDS             complement(14842..15375)
                     /locus_tag="NBI74_003253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCD1933537.1"
                     /translation="MAIKGLDQAIDNLSRVRKNAIPAASAMTINRVATTAINQSSSQV
                     ARETKVRRKLVKERSRLKRATVRNPNAKIIVNRGDLPVIKLGIRMLGRRPNSILKAGQ
                     HRYQRAFIQRLKNGRWHVMQRVAGKNRYPIDVVKIPMAAPLKQAFDENVDRIRRERLP
                     GELASALKQQLRIAIKR"
     gene            complement(15391..15744)
                     /locus_tag="NBI74_003254"
     CDS             complement(15391..15744)
                     /locus_tag="NBI74_003254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001204533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCD1933538.1"
                     /translation="MRDFQNAFDAALAGVDSTIVEVMGIRAQFTSGAQRGGEVQGVFD
                     DPESLGFAGGGVRIEGSSPSLFVRTDTVRAVRRGDTLTINGEMFWVDRVSPDDGGSCY
                     LWLNRGQPPAVNRRR"
     gene            complement(15737..16111)
                     /locus_tag="NBI74_003255"
     CDS             complement(15737..16111)
                     /locus_tag="NBI74_003255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000201530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1933539.1"
                     /translation="MATKEQNLKRLDELALILGREPDISGSAAEIAQRVAEWEEEMQS
                     SGDDVQVMNMDIRERETAAHDVREETSGALTRIRVLTCLHLCGVDGETGESVELADVG
                     RVILIMSSDAKTHVDGGMAVYA"
     gene            complement(16163..>16580)
                     /locus_tag="NBI74_003256"
     CDS             complement(16163..>16580)
                     /locus_tag="NBI74_003256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HCD1933540.1"
                     /translation="WRLLNGFKLFREKLDTRRGSNSQLETAVKDLGAVVSFKGYYGDL
                     AIVVAKMSYIAEDGIEKRYLPDGMLVLGNTAADGIRCYGAIQDAQALSEGVVASSRYP
                     KHWLTVGDPAREFTMTQSAPLMVLPDPDEFVVVQVK"
BASE COUNT         3428 a         5154 c         4034 g         3964 t
ORIGIN      
        1 ttgttcactc attctgaatg ccattatgca agcctcacaa tatagttaaa tgcgatgttt
       61 ttgacggtgt tttccgcgtt acccgcagcg ttaacggtga tggtgtgtcc atgtgaacca
      121 atcgcaacgg agtgcgtatg cgcaccaata cctacagtat gtgcatgtgc gccagaactt
      181 gctgcagtac cagacagcga gtgggtatga gcacctgctg actgtgtctg aatacgttga
      241 taatacgatc tataggaaga agtctccggg cttacttgat actgtgaatt ctggacataa
      301 gtgaacccac cgccatcata aaatgctaac gcagaaccgc cgcctcctgg ccaacgaata
      361 ccattaccat gagtatgaac accggcagac cccgtagagc cactcagact gtgcgtatgc
      421 gccccggtgt tattcgtgga tttagtgccg taatcaaacg acgatgtggt tttcgtcccc
      481 aaatccgtac tggatgcgct ggcgctgtgg gtgtgcgatt ttatgccgtc ctgttcctga
      541 gacaatacgg cccggccact ggcgggtttg cccttgattg tccagccgcg catatctgga
      601 ataacacctg aaggataggc aatagccagt ttcggatatg ctgccttatc aaacgtctgc
      661 ccctgcatga tcgcataacc tgcaggtggt gtatctgatg gccacggcag cggaacacct
      721 ggcggaaacg cttcaatatt tgccgagccg tcaaatttta cgccgttaat tgtccttgca
      781 gtttgcaatt tggttgctgt gcttgcatta cccgataaag atccagtgat accaccactc
      841 gcgtttaatt tagttgcaat tgtaacattg ccagtatggt tactaattat aaacggcctt
      901 aagctattgt gcgtaccaag actgttaccc gaatctgtca acataaaata tgtgtttgat
      961 ccatcatttc ggataaagaa tccatagttg ccataggcaa tacgcagacc atttgctgac
     1021 cttgaaataa cctcaccagc agcagttaaa ccacctgaaa gagtccctcc agttaatgcc
     1081 aatgccccaa tatttgaagg ggtcaatgtg atatttgcac taccatcaaa tgacacaccg
     1141 ttaatcgttc tggctgcctg aagtttcgtg gcggtcgcag cattacctgt agtgttttga
     1201 ttaccggtag cgttgactcc aggcaagttg atatctgccg atccatcaaa tgccacgccc
     1261 ccaatcttac gtgctgtctt gatcttggtc gcagtatctg cgtttccggt caaattacca
     1321 gtaacactac caccaacttt tagtccatta ccgatggaca ccaaccctga tcttaagttt
     1381 atagagaatg gtcttaatga accgatgtct ccattttccc cctccccttc attagtcgga
     1441 ataagatgaa gatgatcttc ggaacgccta aaaataaggc caaaggcttg gttgaatatt
     1501 cgaagagcat ttatcgtgct gatttttaac tgccctccca ttgtgtcgcc agttttttga
     1561 actgaactat ctttaacagt tttaactgcc tttggcgttg ccgccagctt ttcactggtg
     1621 ctgtttgttg cactgctgag ctgtactatc ccctttttcg tcgtgctcgc atcctccagc
     1681 gccacggcgg atgcaatatc ctctgcccgt tttgctgctg tctcggcgcg cgttgccgcg
     1741 gattcagcag caactttgct ctgagatgca gccgtcgcac tgcctgccgc ctctgatgct
     1801 ttcgttgttg ctgtcgtggc actacctttc gctgctgacg cttgtctggt cgcctcatct
     1861 tttgaagcag acgcagatga tgccgatgac gccgctgaac tggctgacga tgcggctgcc
     1921 gccttagagg aagcagcatt gtctgctgaa gtctttgcat ttgtttcaga ggtttttgct
     1981 gcagaagcag acctcgctgc tgcagtggct tgctcagtgg ctttgccagc cttcgttgtg
     2041 gctgttgaag cggatgatgc ggcgctttct gccgattttc cggcggcggt ggcactggct
     2101 gaggcctgcc cggcacttgt tgacgcggca ctggcagata atgcagccgc tgtttttgaa
     2161 cctgccgcag ctgaggcgct ctgtcccgct gctgtttcag aagacttagc gttcgtctcg
     2221 gacgtttttg ccgccttcgc ggaatttcct gccgccgttg ccgaggaagc tgcactactg
     2281 gcacttgatg atgcattcgt ttctgaagat ttcgctgcct cttttgaggc cgccgcaccc
     2341 cgtgccgagg tggcagcttc tgacgccttc atggtcgctg tggatgcaga agtggctgcc
     2401 gatttttgtg atgctgcggc attcgtttct gacgttttcg cggcactggc gctggtagct
     2461 gccgcgcttt ttgatgactc tgcagcagca gcactttttg ctgcttcacg ggcctttgtc
     2521 gatgccgttc ctgcgctgga agacgctgac tgagccgacg acgcggcctg tccggctgac
     2581 gtgctggcgg cacgtgctga ggctgcagca tcggttgcat gagttgccgc ctcgctggct
     2641 gatgcactgg catcgctggc tgattttttc gcggctgccg tattctgtgc aaccgcggag
     2701 gcgttacgtg acacctcttc caccatctgc tcaaagcggc gcagtgcctc cggtcggaca
     2761 tcatcctccg tcatggcacc gagaaaatca ttcagcgtac ctggtctgga accttcatag
     2821 acggtaatgg tcccggcatg tgaaggcgga aaaccttcaa ccagcagggt gacgctgtac
     2881 tggccatgct caacatccat gctgtaacgt ccggcttcat ccggattttc agaggccacc
     2941 gtgttcacca ccaccgtgct gctggttcgt ctggccttca gcacaatggt gcagttctgt
     3001 actggttttc ctgtgccatc tttaagcacg ccagaaattt ttactgtcat acttttccac
     3061 caataaaaaa agcccgcagc agtgacgcca cgggcttcag gacagtgtaa ctttacgttt
     3121 cctcaaacgc agttcacccc ataaggtgga tgaacctgcg tatcataaca atatttacag
     3181 aagataaatc ggcgtctgtt gtcagaaacg gtatccgata ccaacaataa atgcatccgt
     3241 tcgccagtcg ccactaccgg aaccttcata agcaaggtca atggttacgg attcggtcgg
     3301 gttaaactgc acgccagccc cccacgccag agacgtgttg ctgtggcgac cgtcatcact
     3361 tccggtcagc acatcgtgcg ttttcccctt gttgtcagtt acgcggagat aatccccgga
     3421 gaaagtcgac acacggctgt aagccacacc caccatcgca tacgcgctga accattcatt
     3481 cacgcgtaca gacggccccg ccatcacgct gaaccagcgg ttacgcacgg aatcttcatg
     3541 ccagcgggta tcgctgtagt gcgttttttg ctcatcctca gcattggcat aactgaagga
     3601 cgtaatcagc cccagcgcgt ccgtaaactc ataacggtat ttcacgttaa tcccgttcag
     3661 attatcgctg ccgggagcgt tcgtacgggc atgaagatac cccgcgctca gtgtgggctg
     3721 atgttcagac gcccatgcag gcgcaccgga tacggacaga cagatggctg cggacaaaat
     3781 ggctgcacaa actttacgca taattacctc tcgcttttct gcaataaaaa aggcgccatt
     3841 tctggcgccc gtattggggt tataaaattc agctaatcgt gatgcctgca gtggctttct
     3901 tcatcacaac aaccagcaaa tcgctgatac ttgctgtggg ataccagcca tttacccacc
     3961 atgctgatac agaaaactcc agcgtcatgt ggccgcgacc agcaggcata tcaataacac
     4021 cactgtaaat cagcgtatta tccagcgcgg tacggttata aatttcagca ccgtttttcc
     4081 gtactatcag gcggcatgac gaataagtat cgctgttctc ccgctcatgt ctggcaccgc
     4141 tgaaagccac cgccggaata acaatctgcc ggttaaacgg ctgatcgtca taaaccctga
     4201 cggtaatggt tcctgatggc caacgctccg gtgcacggga gtcacggggg aaagccttac
     4261 ccactgtttt aacgagatcg ccttcaatct ggtttgcaga cagtttccct ctgatgacac
     4321 agttctcgtt aatggtgaca ttattgagcg tgccggtatt cgccgtgatg gctccactga
     4381 tatccgcatt gcgggctgtc agcctgccat ccggcgtcag ggaaaacgta ggaggattgc
     4441 cggatgacgt gatgctcacc gcaaacagtc gcttcaggaa cacgtcgttc atgaacagct
     4501 gattcccctg cgccacaaac agcggcgtgg tgttgccatt ctccggggta atcatcgcga
     4561 tacggtccgc ctgcagcaga atactgctca gcgtctgacc atcaacatcc tcaatccccg
     4621 caccaatccc ggccacatag ggaataccgt tttttgtttt ctgcaccttc agcatataca
     4681 tggcattcag ctcattgcgc gtgtctgact gaacccgctg gatttgctgt atggtcacgg
     4741 cctggtcacc cagcttttta tccgtggtcg aggtaatttc actccctttt ttatccacgt
     4801 actggcggac ctgtgctatc tgtcgggcgt tttctgactg cccctggctg acagtctgtg
     4861 agatttcact gctcacccgg tccactttct ggctcacctg cgcgatggcc agtgtctggt
     4921 cctcattctt tttcgcaacc agctgcgtga ggctgttttc cgccttcccg attttccggg
     4981 tcacttctgc gatatccgtg tccatccgct gacggatgtc ttcttccagt tgcgtgacct
     5041 ccgtacgcag cgctgaagca tcaatgcgct ctttcagtgc ctggcccaga agcgtctcat
     5101 ctatcagccc ccggaaaatt tccagatacc cttcaccatc attgctgggc tgcccgctgg
     5161 cttccacaaa agcagatttt cccaccaggt tgacgcttcg cacgtaaaac cagaaatccg
     5221 tccccggctt aatccggctc ccctggacag tccactgact gccggtcccc agataacggg
     5281 cagatttttc cacctgtgcc gtgttcgtga tgcgtttttc tgagaaccag aattcaaact
     5341 gtaccgtcgg gtcatacacc gcaagacgcg ggaccgccgt tatctgaaaa taccccggcg
     5401 tcagctcaat ccgcgacggt gctgccggtg cggcaatccg gaaggtggtg gtcgcaggtt
     5461 cgccctgctg gccgtagctg ttaatggccc gcaccgtcag ggtgtattcc cccagtggca
     5521 ggccgctgaa acggtgctcc gtgtctgcgg tgatggcggt ggtcaccagt ctggcatccg
     5581 ttcccttacc actggtcagg cgcagactga agcgcacgcc cttcaccacc cgcggcgtgt
     5641 cccatttagc ctgcgccaga tactggccgt cagctgcgct cacctccacc gtcaggtgct
     5701 gcactgccgg tgggataacg ctgttcaggg tgcctgactg cggctcaaag cgggccccgt
     5761 tatccacgat ggcttctttt tcaggtacgt gctgcaccgc cgtgatggca aaggtgccgt
     5821 ccgtgttttc ccggatggag acacagcgga acaggcgacg acgcagtgac ggcagggaga
     5881 gtccccacac accgtatgtc tccacaccat ccggcagggt gctgacctgt atccggtcag
     5941 gcgcggggtg tgcggtgatg gccacgctcg ccggcttacc gctgccgtta atcagattaa
     6001 cagtggccgt gccggtctcc ggcagtgtca cctcccggtc cagcgtcagg gtacggctgg
     6061 cggcatcgat ggacaggaca cgtccgccgg tcatggtccc ggcatagtcg ttatcacaga
     6121 tttcaatgat gtcaccgggt gtgtgacgca gcccctgtga cccgagcgtg aaatccaccg
     6181 tctgcgtttc cagcagttcg gtctttatca cccacagccc ggcacggtgg gcctgaccgc
     6241 ggctggtaca gccgaacgcg tccatcttca gcaggttgcg tccgtagcgc agtatggctt
     6301 ccgggtcttc caccagttcc gtggaggtct gccagccgtt ctgcgggtcg gtgtaattca
     6361 cctccactgc cgtgtgccgg tccttcaggg cactgaagct gtagcggaaa cccacgccgt
     6421 tatcatccac caccacatcg ctgttggtgt acggccacac cacatccgac ggacggtcct
     6481 gaacgaacgt cagcgtctgg ccgttccata ccggcataca gcgcatcgca gagcagaaat
     6541 cactgagaac gtcccacgcc ttacgctgtt gtgccaggta cgcattaaag gtcatccgcg
     6601 gctcggtccc cccgaaacca tccgggaccg tctggtcgca gtactgcccg atggcataca
     6661 gcgcccactt gtccacatcc gccgccccca gacgttttcc catgccgtag cgcgggtgag
     6721 tcagcatgtc ccacaggcac caggccgggt tgttgctgta tgccggtttc agactgccgt
     6781 cccagatacc actgtacgtg cgtttttccg ggtcatagtt tgacggcacc tggatgatgc
     6841 gaccgcggat atggtagttc accgtcatct gctggccgcc gaactgctcc gcatccacct
     6901 gcagccccac aatggccgtg ttcgggtagc actgtttcac atcgatgatt tcggtgtatg
     6961 acgaccacag cgtcttattc tgcagctggt ccgtggtgct gtccgccgtc tccctgacca
     7021 tccggatgtt aaagggccgg ggaggcagat tatccagaat caccgaggcc aggaactgcg
     7081 aggtggtctt gccgttaatg gtgacatcct tttccgtcac ccagcggcca ttacgctgta
     7141 actgaatcag aatccggaca gaggaaggat tacggtcgcc ctttgacgtg gtctgcacca
     7201 gtgactgcac cccgaaggta acccgcaggc ggtcaatgtt cgcggacgta atggtgcgcg
     7261 tcaccggttt tgccttcgtc acttccacgc ccagtccggt ttcagcaccg gaggactcaa
     7321 agccttccgg tggtgtctgc tcctgctccc cggcgcgcca gaccgccgtc acaccgtgta
     7381 tcacgggatt accgtccgtg tccgtcagcg gggttttgtt caccaggata ctctgcagtc
     7441 ccttcaccgg accttctatc ggtccctcac caatcgcatc aatcacgctc atcatctgcg
     7501 tggatttgag attatccttc gcctcacgag gcgtgtgtgc cttaccgcca ccttttccca
     7561 tacagccttc ccctgaataa attaaccgcc acttgccatt ccgtacagaa gtcggatatc
     7621 cttcgcccga aaagcatgaa acacatttct gccatgctaa agagaaaccc cggtatcagc
     7681 agataccggg gttttctttc atgcccaccg ataatcctgt tggttaaaac cggtaatggc
     7741 ataaaaattc tgaatatctt cacattttca caaactgact gtggcgcgta taatttctct
     7801 gcgttaattt ttttgtcgtg atataagaat aattccttac acttaatctt cgtaactctc
     7861 ccgcagttcc tgtccgcgat cactgcggga tttttttatt ctttttaccc ctgccgcccg
     7921 ataaccacga cctttccgcc cccgccttca tcacgggtgc tgatgtcctg ggatatacgg
     7981 cgggagccaa ccagcatttc cccgtaaggc accggcatcg ggttcccctg ggcaatcatg
     8041 ttatccagcg aggaaaagta cgtgttctgt ctgccgttat ccgttgcgcg gtaatccggt
     8101 gtttttgcct tcggggccag catctgggcc acaccgccca gtatcatgct ggcacccagt
     8161 gaaaacagca ttgtggtggc agaaaaaccg ccggcactca gggctgtacc ccataacgcc
     8221 atcgaggcac cggccgtgaa gaaagagccc acgatggctg ccgcccccag cacaatctgc
     8281 agtccaccct ttccggcccc ggccagtcgc ggcacaatgt ggatgaccgt tccctcaccc
     8341 agctgttcgt gaagacgggc gtacaccgcc tccggtgccg tgtcatcacc ggcaatacgt
     8401 atctggtacc agccttcgtt catctgacgg cgaaagcccg gcatctgcat cgacagggcg
     8461 cgaatggctt ccgctgccgt gttcacatac aggctgaggc ggcggccaaa tcgttgtaaa
     8521 tccccgtgaa ggcagatgcg tgccagtggc ggtgacgcca gacagaatgc gttcgtcgtt
     8581 gccatttttc ggaatacctc tcccgtttac tcagttgttc aggcagatgg tgaagcagct
     8641 caccgttacc gcagtaaatg gcggcatggt tcggtaccga agcaccaaag cagcacagca
     8701 gaatatcgcc cgcctgtgca gaggacaggg gcacccggta aaagccggtg accgccatat
     8761 tgtccaggta aaggttctgg ccgttgcgcc accagtcatc ctcgcgatga aaatccggca
     8821 tttcagtccc cgccagatga taagcatccc ggaacagcgt gtaacagtcc gtcaccccgt
     8881 gctcaaagcg ccgtcccgtc agatgtggca cacagcggaa tttgtgaatg tcaccccggc
     8941 agaccagcca ccagggcagt gcgcttttta tctgcagccg ccggtcagcc tcgctcagcc
     9001 agggcagccc accgggatga ctgtggacca gtgccacaat ctccccctgc atctctgccc
     9061 gcagccagtc ttccggtgcg atacgaaaat acgcctccgg ctccgcggaa atattcacac
     9121 aagggatata ccactccccc tccggcgtgc ttatcacgaa gccgcacgac tccgcaggcg
     9181 cacaccgccg ggcatgtgcc agaatcgctg attcagtctg tgtcataaac cgggatttac
     9241 tgcgaaagtt tattaatgga aaggaaaccg ccaaaattgc cgacattcct gcgcagttca
     9301 cacccgcgca tgcacttgct gcatctgtcc ttacggatat ccgtggtggg tttatcgaac
     9361 tcatccgcca cagccccgcc cgtgtaacca cactcatcag agcggtaggt ccacatacag
     9421 gtgttcgcca gcatgatacg accgggaaac agcgccccgt ccgtctcggt cggtgtggcc
     9481 agcacaaacg aggccgtcat ggctgtcagc gctgacatct gctccaccac ccaccggtcc
     9541 gtcagctcct gctccgggtc ggcctccgga ttgcccgcaa cgaaattcac cgcatccaga
     9601 aaacgggcat acacccggcg gcggaccacc gtggccccca ccagactctg caggtcttcc
     9661 gccatcccgg tgaccagacc gaacagattg gacaccgtca gcgacggtct ggcactgctg
     9721 cccttcccgc tcatctcaaa gccactcccc tcaatcgggt acgcctgata ttcacgcccc
     9781 tgccaggtca ccggctcccc tttttcattc agctcattgc agaaaaaata ccgctcaccg
     9841 ccctgcaccg tcaggtcgat ttcccagagt accacccgcg gtgactgctc tgacttaacc
     9901 gactcgttca gactttcttc gtgaatatcc tgcatcagtt caccacctgc ttaaactccg
     9961 cgctgaactc aacgcgcaac atcccgaccc gcgcagacca cccggcacag atcaccttta
    10021 tctgccggta tgcatagggt ggcttccaca aaaatgcctt ccagccaccg tgctctgcca
    10081 ggaacgcttc cagatgccgg gcctcctccc gggtcacgga aagcgtcacc ctgtatgttt
    10141 tcaggtcagc attcagccct gccgccatac gctgtgagta cccgtcacca aaacgcactt
    10201 cacgcaccga tggctgcgag ttcacctcca tatccggctt cactttccag cgaaatgttt
    10261 tcatcgcccg ctccccgata acagaccgcc atcacgcaac tgcagccgga gctcatcctg
    10321 cgcaccttta cgggccatct catacaccgc tttcatcagc tgcggcccgg cctgtccgtt
    10381 gataccgtcg ttctgaatca ccacgtgatt gttctgatta aaattaatgc cttcggcccg
    10441 ccgcatctgc gccggacttc cggcagcacc cacatacccc ccttccgcat agccccgcat
    10501 caggcggtac aggttcccga caccaatccg gctggttgcc tccttcgtga agacaaactc
    10561 cccgcggtgg acaatccccg ctggctcata tttgccgccg gttcccgtaa atcctccggt
    10621 cgcaaaatgg aatttcgccg cagctgcctg aatggctgta ccgcctgacg cggatgcgcc
    10681 accaccaaca gccccgccaa tagcgctgcc gatactcccg acaatcccca ccattgcctg
    10741 cttcagcaga atttctgtca tcatggacag cacggagcgg gtgaagctgc gccagttctg
    10801 ctcactgccg gtcagcatcg ccgccatatt ctgtgcaata ccatcaaagg tctgcgtggc
    10861 agcacttttt acctgcgaca tactgtccgt ggcactctct tcccactcac tccagccgga
    10921 cctgaggcct gccatccagt tcccgcgaag caggtcttca gccgcccagg tctttttctg
    10981 ctctgacatg acgttattca gcgccagcgg attatcgcca tactgttcct tcaggcgctg
    11041 ttccgtggct tcccgttctg cctgccggtc agtcagcccc cggcttttcg catcaatggc
    11101 ggcccgtttt gcccgttgct gctgtgcgaa tttatccgcc tgctgcgcca gcgcgttcag
    11161 gcgctcctga tacgtaacct tgtcgccaag tgcagccagc tggcgtttgt actccagcgt
    11221 ctcatcttta tgcgccagca gggatttctc ctgtgcagac agctggcgac gttgcgccgc
    11281 ctcctccagt accgcgaact gactctccgc cttccacaaa tcccggcgct gctggctgat
    11341 tttctcattc gctccggcat gcttctccag cgtccggagt tctgcctgaa gcgtcagcag
    11401 ggcagcatga gcactgtctt cctgacgatc gcccgcagac accttcacgc cggactgttt
    11461 cggctttttc agcgtcgctt cataatcctt tttcgccgcc gccatcagcg tgttgtaatc
    11521 cgcctgcagg attttcccgt ctttcagtgc cttgttcagt tcttcctgac gggcggtata
    11581 tttctccagc ggcgtctgca gccgttcgta agccttctgc gcctcttcgg tatatttcag
    11641 ccgtgacgct tcggtatcgc tctgctgctg cgcatttttg tcctgttgag tctgctgctc
    11701 agccttcttt cgggcggctt caagcgcaag acgggccttt tcacgatcat cccagtaacg
    11761 cgcccgcgct tcatcgttaa caaaataatc atccttgcgc agattccaga tgtcgtctgc
    11821 tttcttaaac gcagcctctg ccttaatcag catctcctgc gcggtatcag gacgaccaat
    11881 atccagcacc gcatcccaca tggatttgaa tgcccgcgct gtcctgtctg cccaggtctc
    11941 cagcgtgccc atgttctctt tcaggcggcg ggtctggtca tcaaaccctt tcgttgcggc
    12001 ctcgttcgcc gcctgcaatg ccccggtttc atcgccggaa cgctgcaact gagcaacata
    12061 cgcaatctgc tccgccgtca cgttatggaa ctgcttcgcc atcgcaatca gccccgacgt
    12121 cgggtcagtg gtcagttttc cgaaagcctc tgcaaccttg tccacctcca caccggatgc
    12181 agaagcaaaa cgcgcgacac tctggttgat ggcatcaaac tgttcaccac cacgcacacc
    12241 ggcattcacc atggctgcca gtgactcact cgcctggtta aacgtcagcc ctgcggcctg
    12301 tccggctctg gagagcgtca gcatgcgatc ggcagtcagt ccggactgat taccggaaag
    12361 aaccagggtt ttattaaacg ctgaaagcgt ggaatctccc tggtaccagg cgtacaccag
    12421 cgcacctgtc gccaccgcca gcgaggtgac cccgaccatc ggcagggtga tcgcaccggc
    12481 aagccccctg aacatgggga tcatcccgcc gaaggagtcc ttcacctgac cgccctgttg
    12541 cagcaggatc agccagggat tctgaccacc ggcaagctgc gtggcgatat ccgtaaactg
    12601 tgcgggcagg gttcgcatgg ccgctttata ctgcccgacg gaaatccctg ctttttgtgc
    12661 agccagcgcc tggtggctca gcccctgctc aacgacagtt gcggtttttc tggcgtcagt
    12721 atccagacct gaaaaatgac gccttacccg gctcatctgc tcatcgaaac ggaccgcatc
    12781 cagactcagg tcaataacaa gatcaccaac cggctgggac atatctcaca cctcccggaa
    12841 tccccgctga agccatcatt aatgcggcat catccaccat gacatccgcc acatccgcag
    12901 acgataaaat atcgccccct ccgtccccac cgaaccggac gcctccggca agtcctgccg
    12961 ctttctgcat cagcattttg tcctcatccg gcctctccac ctgctcttcc tcatgccggg
    13021 ggacaagcag actgaaatca gagggatgca tatccggatc gcaaaaaaac aggctgagta
    13081 cagcgtacgt cagcccggaa aaatgcatat ccagctgggt atcctgaaaa taatgcgtgc
    13141 ggtaaaaacg gtgccagtcg gcatattcgg tggatgtcat cccggcaagc atggcgcgcc
    13201 agtcgggtct ccccatctca cgcgccagtc tgagggcaaa gttcagctca ccgtcgaaga
    13261 ctttcccgca gaaaaatcat catcagtcag cgtgttattt ttcgccactt cagtaatatc
    13321 agtatccgga cgaacagctt cgatcatccc ggacagacgc aacaccacgt cttccgcccg
    13381 ggcaatggca tcagcaggcc aggtggtgag cacttcctgc tctatcttca tcacggcctc
    13441 attcattgac ggtgactgcg ttttctgtgg atggttatgc cacagggaca tcgccaccag
    13501 aaacgcgccg gttctgacaa gatcttccac actcacctgc aggttgccgc tggcttcagc
    13561 ctcttctgcc cgccgtttca ggagggcaag atgctcaata cgctgcagcg cagacagctc
    13621 agaaagcgtg acggatacac cgttatattc aaattgttct gttttcagga acatcgctta
    13681 tctctcagct ctttagctac ccggcacatt attaacggta atttcagcca ccgcagcaaa
    13741 ctgaccatta ccggaaatca cagggatgct gacttttcca tccttaaccc ccgtcacagt
    13801 aatcgtcata tctttcacgc taatggtggc ttttgatgga tcggcggaaa tcgccctgaa
    13861 tgtcttatcc gttgcatttt ccggttccac agtaacggtc agggtggttg ttttcccttt
    13921 tgccaccgta ccggatgtcg gcgtcacctt aatcgcactg accggcgtaa ttttgctgcg
    13981 ttcttccgct acagaaggtt tacccacgtt agtgactttc accgtgcggg tgatcacttc
    14041 tttcgccgtc acggccttac cgatactgct gacccagcca cgaaacacat ccaccgtgcc
    14101 attcggaaaa cggattttat aggcccggac atcgccgctt tcaaaccagc ctataagccc
    14161 tttctgacct tcctctcccg gtttccaggc cagcgtaaaa ctggtatcac ctgcagattt
    14221 ctgcccctgc ccggtcgcgg tccagtctgc gtcttcatca tccaggtagt tatcatcgta
    14281 ggattctgcc gtcatctcgc ccggcgtcag atccttcacc ttagccagtc gctgccagtc
    14341 agcgtctgac aacgggtttg cataagcatc acccttgccg ttgtaaaccc acagagtggt
    14401 accggcacct tttaccggct ccaggggatt tggtgttgcc atatcgtcct cacatctcgt
    14461 atgtaatgga ataagtcaga tccgcagagc tccataacgc catatcgtca tcacgacgat
    14521 actcatagcc ctgcgtaacc atcgtggtaa tcagtcctgc cagtgccggg atcgcagtca
    14581 tcgccggata aatccggctt tccatccact gatcaagctc tgaatccggt acctgtgccg
    14641 gtaaaaacac ctcaatatgc agtgtggccc gccaggtatc tgcatccagc tcttcaccgg
    14701 tatactctgc atccgtcaga taaaccgcga tcgcaggaaa atcctcttcg tcaaaaacaa
    14761 cggggcgacc atcaaacagc gtcgccccgt gttcatgctg ctcgagtgca tccagcactg
    14821 cagcacggat atcagtgtgt ttcatcgttt tatcgcaatc ctcagttgtt gtttcagcgc
    14881 ggatgccagt tctccgggca ggcgttcacg ccggatacgg tcaacattct catcaaacgc
    14941 ctgtttcagt ggggccgcca tcgggatttt caccacatca atagggtaac ggtttttccc
    15001 ggccacacgc tgcatgacat gccagcgacc gttttttaat cgctgaatga atgcccgctg
    15061 ataccgatgc tgaccggctt taagtatgct gttcggacga cggcccagca tcctgatccc
    15121 cagcttaatc actggaagat caccgcggtt aacgataatt tttgcgttcg gatttctgac
    15181 cgtcgcccgt ttcagtctgg accgttcctt aaccagtttc cggcgtacct ttgtctcccg
    15241 ggcaacctgt gatgaagact gattaatcgc cgttgtggcc acgcggttaa tcgtcattgc
    15301 tgaagccgcc ggaatggcgt ttttacgaac ccggctcaga ttatcaatcg cctgatcaag
    15361 cccttttatc gccataattt ccccctgcgt ttatcgtcgc cggttaacag cgggtggttg
    15421 cccacggttg agccagagat aacagcttcc cccgtcatcc ggagaaacac gatccaccca
    15481 gaacatctcg ccgttaatgg tcagcgtgtc accacgccgc acggcacgca ccgtatccgt
    15541 ccgcacaaat aatgacgggc tgcttccttc aatacggacc ccgccaccgg caaaacccag
    15601 cgactccgga tcgtcaaaaa ccccctgaac ttcgccgcca cgttgtgctc cggaggtgaa
    15661 ctgcgcacgg atccccatca cttcaacaat cgtactgtcc accccggcaa gggcggcatc
    15721 aaaggcattc tgaaaatcac gcataaacag ccattccacc atcaacgtgt gtttttgcat
    15781 ctgaggacat aatcagaatc acccgaccaa catccgcaag ctcaacggat tcccccgttt
    15841 caccatcaac gccacagaga tggaggcagg tcagaactct gatgcgcgtt aacgcgccgg
    15901 atgtttcctc acgaacatca tgagccgcgg tttcccgctc ccggatatcc atattcataa
    15961 cctgtacatc atcgccggat gactgcattt cctcttccca ttctgccacc cgctgcgcta
    16021 tctctgcggc actcccggat atatccggct cacgccccag aatcagggcc agttcatcaa
    16081 gccgtttcag attttgctct ttcgttgcca tatcagcccc ctgtgaaaaa agacacgggg
    16141 gcatttcgcc cccgctcacg gattatttca cctgtaccac cacaaactca tccgggtccg
    16201 gcaacaccat cagcggcgcg gactgcgtca tggtaaattc acgggcggga tcccctaccg
    16261 tcagccagtg tttcggataa cgggaagagg ccaccacacc ttcggacaac gcctgagcat
    16321 cctgaatggc accgtaacaa cggatcccat ctgcagcagt attccccaga accagcatgc
    16381 catctggaag ataacgtttt tcgataccgt cttctgctat ataagacatt ttcgccacca
    16441 caatggccag atcgccgtaa taccccttga aggacaccac tgcgcccaga tctttcactg
    16501 ccgtttcgag ttgagaattt gaaccgcgac gggtatccag tttttcgcgg aacagcttaa
    16561 aaccattcag aagacgccag
//
LOCUS       DAHHTG010000098        15968 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.098, whole genome shotgun sequence.
ACCESSION   DAHHTG010000098 DAHHTG010000000
VERSION     DAHHTG010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15968)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15968)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15968
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.098"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            217..987
                     /locus_tag="NBI74_003257"
     CDS             217..987
                     /locus_tag="NBI74_003257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter ThrE family protein"
                     /protein_id="HCD1933541.1"
                     /translation="MQTEQQRAVTRLCIQCGLFLLQHGAESALVDELSSRLGRALGMD
                     SVESSISSNAIVLTTIKDGQCLTSTRKNHDRGINMHVVTEVQHIVILAEHHLLDYKGV
                     EKRFSQIQPLRYPRWLVALMVGLSCACFCKLNNGGWDGAVITFFASTTAMYIRQLLAQ
                     RHLHPQINFCLTAFAATTISGLLLQLPTFSDTPTIAMAASVLLLVPGFPLINAVADMF
                     KGHINTGLARWAIASLLTLATCVGVVMALTIWGLRGWV"
     gene            978..1451
                     /locus_tag="NBI74_003258"
     CDS             978..1451
                     /locus_tag="NBI74_003258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter"
                     /protein_id="HCD1933542.1"
                     /translation="MGVIEFLFALAQDMILAAIPAVGFAMVFNVPVRALRWCALLGAI
                     GHGSRMILMTSGLNIEWSTFMASMLVGTIGIQWSRWYLAHPKVFTVAAVIPMFPGISA
                     YTAMISAVKISQLGYSEPLMITLLTNFLTASSIVGALSIGLSIPGLWLYRKRPRV"
     gene            1558..2097
                     /gene="dnaT"
                     /locus_tag="NBI74_003259"
     CDS             1558..2097
                     /gene="dnaT"
                     /locus_tag="NBI74_003259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaT"
                     /protein_id="HCD1933543.1"
                     /translation="MSSRVLTPDVVGIDALVHDHQTVLAKAEGGVVAVFANNAPAFYA
                     VTPARLAELLALEEKLARPGSDVALDDQLYQEPQAAPVAVPMGKFAMYPDWQPDADFI
                     RLAALWGVALREPVTTEELASFIAYWQAEGKVFHHVQWQQKLARSLQIGRASNGGLPK
                     RDVNTVSEPDSQIPPGFRG"
     gene            2100..2837
                     /gene="dnaC"
                     /locus_tag="NBI74_003260"
     CDS             2100..2837
                     /gene="dnaC"
                     /locus_tag="NBI74_003260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein DnaC"
                     /protein_id="HCD1933544.1"
                     /translation="MKNVGDLMQRLQKMMPAHIKPAFKTGEELLAWQKEQGAIRSAAL
                     ERENRAMKMQRTFNRSGIRPLHQNCSFENYRVECEGQMNALSKARQYVEEFDGNIASF
                     IFSGKPGTGKNHLAAAICNELLLRGKSVLIITVADIMSAMKDTFRNSGTSEEQLLNDL
                     SNVDLLVIDEIGVQTESKYEKVIINQIVDRRSSSKRPTGMLTNSNMEEMTKLLGERVM
                     DRMRLGNSLWVIFNWDSYRSRVTGKEY"
     gene            2886..3380
                     /locus_tag="NBI74_003261"
     CDS             2886..3380
                     /locus_tag="NBI74_003261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2501 domain-containing protein"
                     /protein_id="HCD1933545.1"
                     /translation="MMKTVKHLLCCAIAASALISTGVHAASWKDALSSAASELGNQNS
                     TTQEGGWSLASLTNLLSSGNQALSADNMNNAAGILQYCAKQKLASVTDAENIKNQVLE
                     KLGLNSEEQKEDTNYLDGIQGLLKTKDGQQLNLDNIGTTPLAEKVKTKACDLVLKQGL
                     NFIS"
     gene            3634..5925
                     /gene="opgB"
                     /locus_tag="NBI74_003262"
     CDS             3634..5925
                     /gene="opgB"
                     /locus_tag="NBI74_003262"
                     /EC_number="2.7.8.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313346.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol--membrane-oligosaccharide
                     glycerophosphotransferase"
                     /protein_id="HCD1933546.1"
                     /translation="MSELLSFALFLASVLIYAWKAGRNTWWFAATLTVLGLFVVLNIT
                     LFASDYFTGDGINDAVLYTLTNSLTGAGVSKYILPGIGIVLGLTAVFGALGWILRRRR
                     HHPHHFGYSLLALLLALGSVDASPAFRQITELVKSQSRDGDPDFAAYYKEPSKTIPDP
                     KLNLVYIYGESLERTYFDNEAFPDLTPELGALKNEGLDFSHTQQLPGTDYTIAGMVAS
                     QCGIPLFAPFEGNASASVSSFFPQNICLGDILKNSGYQNYFVQGANLRFAGKDVFLKS
                     HGFDHLYGSEELKSVVADPHYRNDWGFYDDTVLDEAWKKFEELSRSGQRFSLFTLTVD
                     THHPDGFISRTCNRKKYDFDGKPNQSFSAVSCSQENIAAFINKIKASPWFKDTVIVVS
                     SDHLAMNNTAWKYLNKQDRNNLFFVIRGDKPQQETLAVKRNTMDNGATVLDILGGDNY
                     LGLGRSSLSGQSMSEIFLNIKEKTLAWKPDIIRLWKFPKEMKEFTIDQQKNMIAFSGS
                     HFRLPLLLRVSDKRVEPLPESEYSAPLRFQLADFAPRDNFVWVDRCYKMAQLWAPELA
                     LSTDWCVSQGQLGGQQIVQHVDKTTWKSKTAFKDTVIDMARYKGNVDTLKIVDNDIRY
                     KADSFIFNVAGAPEEVKQFSGISRPESWGRWSNAQLGDEVKIEYKHPLPKKFDLVITA
                     KAYGNNASRPIPVRVGNEEQTLVLGNEVTTTTLHFDNPTDADTLVIVPPEPVSTNEGN
                     ILGHSPRKLGIGMVEIKVVEREG"
     gene            complement(6065..7087)
                     /gene="lgoD"
                     /locus_tag="NBI74_003263"
     CDS             complement(6065..7087)
                     /gene="lgoD"
                     /locus_tag="NBI74_003263"
                     /EC_number="1.1.1.414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-galactonate oxidoreductase"
                     /protein_id="HCD1933547.1"
                     /translation="MSTMNVLICQQPKELVWKQREIPIPGDNEALIKIKSVGICGTDI
                     HAWGGNQPFFSYPRVLGHEICGEIVGLGKNIADLKNGQQVAVIPYVACQQCPACKSGR
                     TNCCEKISVIGVHQDGGFSEYLSVPMANILPADGIDPQAAALIEPFAISAHAVRRAAI
                     APGEQVLVVGAGPIGLGAAAIAKADGAQVVVADTSPARREHVATRLELPVLDPSAEDF
                     DAQLRAQFGGSLAQKVIDATGNQHAMNNTVNLIRHGGTVVFVGLFKGELQFSDPEFHK
                     KETTMMGSRNATPEDFAKVGRLMAEGKITADMMLTHRYPFATLAETYERDVINNRELI
                     KGVITF"
     gene            7226..8140
                     /locus_tag="NBI74_003264"
     CDS             7226..8140
                     /locus_tag="NBI74_003264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCD1933548.1"
                     /translation="MSRSQNLRHNVINQVIDDMARGHIPSPLPSQSALAEMYNISRTT
                     VRHILSHLRECGVLTQVGNDYVIARKPDHDDGFACTTASMSEQNKVFEQAFFTMINQR
                     QLRPGETFSELQLARAAGVSPVVVREYLLKFGRYNLIQSEKRGQWSMKQFDQSYAEQL
                     FELREMLETHSLQHFLNLPDHDPRWLQAKTMLERHRLLRDNIGNSFRMFSQLDRDFHS
                     LLLSAADNIFFDQSLEIISVIFHFHYQWDESDLKQRNIIAVDEHMTILSALICRSDLD
                     ATLALRNHLNSAKQSMIRSINENTRYAH"
     gene            8355..9716
                     /locus_tag="NBI74_003265"
     CDS             8355..9716
                     /locus_tag="NBI74_003265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCD1933549.1"
                     /translation="MEKENITLDPRSSFTPSSSADIPVPPDGLVQRSTRIKRIQTTAM
                     LLLFFAAVINYLDRSSLSVANLTIREELGLSATEIGALLSVFSLAYGIAQLPCGPLLD
                     RKGPRLMLGLGMFFWSLFQAMSGMVHSFTQFVLVRIGMGIGEAPMNPCGVKVINDWFN
                     IKERGRPMGFFNAASTIGVAVSPPILAAMMLVMGWRGMFITIGVLGIFLAIGWYMLYR
                     NREHVELTAVEQAYLNAGSVNARRDPLSFAEWRSLFRNRTMWGMMLGFSGINYTAWLY
                     LAWLPGYLQTAYNLDLKSTGLMAAIPFLFGAAGMLVNGYVTDWLVKGGMAPIKSRKIC
                     IIAGMFCSAAFTLVVPQATTSMTAVLLIGMALFCIHFAGTSCWGLIHVAVASRMTASV
                     GSIQNFASFICASFAPIITGFIVDTTHSFRLALIICGCVTAAGALAYIFLVRQPINDP
                     RKD"
     gene            complement(9765..11429)
                     /gene="tsr"
                     /locus_tag="NBI74_003266"
     CDS             complement(9765..11429)
                     /gene="tsr"
                     /locus_tag="NBI74_003266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HCD1933550.1"
                     /translation="MLKRIKIVTSLLLVLAVFGLLQLTSGGLFFNALKNDKENFTVLQ
                     TIRQQQSTLNGSWVALLQTRNTLNRAGIRYMMDQNNIGSGSTVAELMQSASISLKQAE
                     KNWADYEALPRDPRQSTAAAAEIKRNYDIYHNALAELIQLLGAGKINEFFDQPTQGYQ
                     DGFEKQYVAYMEQDDRLYDIAVSDNNASYSQAMWILVGVMIVVLAVIFAVWFGIKASL
                     VAPMNRLIDSIRHIAGGDLVKPIEVDGSNEMGQLAESLRHMQGELMRTVGDVRNGANA
                     IYSGASEIATGNNDLSSRTEQQAASLEETAASMEQLTATVKQNAENARQASHLALSAS
                     ETAQRGGKVVDNVVQTMRDISTSSQKIADIISVIDGIAFQTNILALNAAVEAARAGEQ
                     GRGFAVVAGEVRNLAQRSAQAAREIKSLIEDSVGKVDVGSTLVESAGETMAEIVSAVT
                     RVTDIMGEIASASDEQSRGIDQVGLAVAEMDRVTQQNAALVEESAAAAAALEEQASRL
                     TEAVAVFRIQQQQQQQRETSAVVKNVTPATPRKMAVADSGENWETF"
     gene            11806..13956
                     /gene="btsT"
                     /locus_tag="NBI74_003267"
     CDS             11806..13956
                     /gene="btsT"
                     /locus_tag="NBI74_003267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate/proton symporter BtsT"
                     /protein_id="HCD1933551.1"
                     /translation="MDTKKIFKHIPWVILGIIGAFCLAVVALRRGEHVSALWIVVASV
                     SVYLVAYRYYSLYIAQKVMKLDPTRATPAVINNDGLNYVPTNRYVLFGHHFAAIAGAG
                     PLVGPVLAAQMGYLPGTLWLLAGVVLAGAVQDFMVLFISSRRNGASLGEMIKEEMGPV
                     PGTIALFGCFLIMIIILAVLALIVVKALAESPWGVFTVCSTVPIALFMGIYMRFIRPG
                     RVGEVSVIGIVLLVASIYFGGVIAHDPYWGPALTFKDTTITFALIGYAFVSALLPVWL
                     ILAPRDYLATFLKIGVIVGLALGIVVLNPELKMPAMTQYIDGTGPLWKGALFPFLFIT
                     IACGAVSGFHALISSGTTPKLLANETDARFIGYGAMLMESFVAIMALVAASIIEPGLY
                     FAMNTPPAGLGITMPNLHEMGGENAPIIMAQLKDVTAHAAATVSSWGFVISPEQILQT
                     AKDIGEPSVLNRAGGAPTLAVGIAHVFHKVLPMADMGFWYHFGILFEALFILTALDAG
                     TRSGRFMLQDLLGNFIPFLKKTDSLVAGIIGTAGCVGLWGYLLYQGVVDPLGGVKSLW
                     PLFGISNQMLAAVALVLGTVVLIKMKRTQYIWVTVVPAVWLLICTTWALGLKLFSTNP
                     QMEGFFYMASQYKEKIANGTDLTAQQIANMNHIVVNNYTNAGLSILFLIVVYSIIFYG
                     FKTWLAVRNSDKRTDKETPYVPIPEGGVKISSHH"
     gene            14006..14209
                     /locus_tag="NBI74_003268"
     CDS             14006..14209
                     /locus_tag="NBI74_003268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HCD1933552.1"
                     /translation="MFGNLGQAKKYLGQAAKMLIGIPDYDNYVEHMKTNHPDKPYMSY
                     EEFFRERQNARYGGDGKGGMRCC"
     gene            14220..15176
                     /gene="yjiA"
                     /locus_tag="NBI74_003269"
     CDS             14220..15176
                     /gene="yjiA"
                     /locus_tag="NBI74_003269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HCD1933553.1"
                     /translation="MNPIAVTLLTGFLGAGKTTLLRHILNEQHGYKIAVIENEFGEVS
                     VDDQLIGDRATQIKTLTNGCICCSRSNELEDALLDLLDNLDKGNIQFDRLVIECTGMA
                     DPGPIIQTFFSHEILCQRYLLDGVIALVDAVHADEQMNQFTIAQSQVGYADRILLTKT
                     DVAGEAEKLRERLARINARAPVYTVTHGDIDLGLLFNTNGFMLEENVVSTKPRFHFIA
                     DKQNDISSIVVELDYPVDISEVSRVMENLLLESADKLLRYKGMLWIDGEPNRLLFQGV
                     QRLYSADWDRPWGDEKPHSTMVFIGIQLPEEEIRAAFAGLRK"
     gene            complement(15210..15512)
                     /locus_tag="NBI74_003270"
     CDS             complement(15210..15512)
                     /locus_tag="NBI74_003270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004020443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnA antitoxin family protein"
                     /protein_id="HCD1933554.1"
                     /translation="MSMVKHKRGTSSTLDVQHEAELKALANKSDEDIDYSDIPPSSDE
                     QWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKGYQTRLNAILREAMLRDQNKK"
     gene            complement(15499..15786)
                     /locus_tag="NBI74_003271"
     CDS             complement(15499..15786)
                     /locus_tag="NBI74_003271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnT family toxin"
                     /protein_id="HCD1933555.1"
                     /translation="MPMEFEWDENKAKSNRVKHGIRFEDAVLLFDDPQHLSQQERIEN
                     GEYRWQTIGLVYGIVVILVAHTIRFESGNEIIRIISARKADRKERNRYEHG"
BASE COUNT         3771 a         4235 c         3965 g         3997 t
ORIGIN      
        1 ggctaaaaca ataaattcat tatagtgata atattcatgt gatagtttgt ttgcggcgag
       61 agataattcg ctttttatca ccgagcttgt cccgataacc aggattaatc agcgcaattt
      121 tgatcttaac ctaccgcaaa acaaccgtgc ttcatgatac aaatggattc tcattatctg
      181 aacaatacgc agcacgtcta tgacaaaggg cgagttatgc aaactgagca acagcgagcc
      241 gtaacgcggc tttgtatcca gtgtggatta tttcttttgc aacatggtgc ggaaagcgcg
      301 ttggttgatg agctttcctc acgactgggt cgggcgttgg gaatggacag cgtcgaaagt
      361 tctatctctt cgaacgccat agtgctgaca actattaaag atgggcaatg cctgacatcg
      421 acacgaaaaa atcacgatcg cggcattaat atgcatgtgg tgactgaagt ccagcacatt
      481 gtgattcttg cggagcatca tctgctggat tacaaaggcg tagagaaacg atttagccaa
      541 attcagccat tacgttaccc aagatggctg gtagccttaa tggttggcct ttcttgcgcc
      601 tgtttctgta aactcaataa cggtggctgg gatggtgccg tcatcacctt ctttgccagt
      661 acgaccgcga tgtatatccg ccagctgctg gcacaacgtc atcttcatcc acagatcaac
      721 ttttgcctta ccgctttcgc cgccaccacc atttccggat tgcttttgca actccccact
      781 ttcagcgata cacccaccat tgcgatggcc gccagcgttc tgctgctggt gccgggcttt
      841 ccgttgatta atgccgtcgc cgatatgttt aaaggccaca tcaataccgg actggcacgc
      901 tgggcgatcg ccagtctgct gacgctggct acctgcgtcg gcgtggtgat ggcactgacg
      961 atttgggggc tacgcggatg ggtgtgatcg agtttctgtt tgcattggcg caggatatga
     1021 tcctcgccgc cattccagcg gtcggctttg cgatggtgtt caacgttccc gtgcgggcgt
     1081 tacgctggtg tgcgctgctt ggcgcgattg gccatggttc acgaatgatc ttaatgacca
     1141 gcgggctgaa tattgagtgg tcaactttta tggcttctat gctggtcggc accattggta
     1201 ttcaatggtc gcgctggtat ctggcgcatc cgaaagtgtt taccgtggcg gccgttatcc
     1261 ctatgttccc gggcatatcg gcttataccg caatgatttc ggcggtaaaa atcagccagt
     1321 taggttacag cgaaccgttg atgattaccc tgttaaccaa ctttcttaca gcttcatcga
     1381 ttgttggtgc gttatccatc ggtctttcca ttcctggatt atggttgtac cgcaagcgcc
     1441 ctcgcgtata aaattgcctc gcttaagcac acggatgaga gacagcctcc tctcctccgt
     1501 gtgttactat aaaagttatc tcccttctcg ttcatcgttc catatttgag aaacagtatg
     1561 tcttccagag ttttgacccc ggacgtcgtt ggtattgacg ccctggtaca cgatcaccaa
     1621 accgttctgg caaaagctga aggcggtgtg gttgccgtat ttgctaacaa tgccccggcg
     1681 ttttatgccg tcacgcctgc acgcctggct gaactgctgg cgctggaaga aaagctggcg
     1741 cgtccgggaa gcgatgtcgc tctggacgat caactctatc aggaaccgca agccgctccc
     1801 gttgctgtac ccatggggaa attcgccatg tatccggact ggcaacccga tgccgacttt
     1861 atccgcctgg cggcgctatg gggcgtggca ctaagagagc cggtgaccac cgaagaactg
     1921 gcctcattca ttgcctactg gcaggcggaa ggtaaagtct ttcaccatgt gcagtggcaa
     1981 caaaaactgg cgcgcagcct gcaaatcggt cgtgccagca acggcggact gccgaaacga
     2041 gatgtgaata cggtcagcga acctgacagc caaattccac caggattcag agggtaacga
     2101 tgaaaaacgt tggcgacctg atgcaacgcc tgcaaaaaat gatgcctgcc catatcaaac
     2161 ctgcattcaa aacgggtgaa gagctactgg cctggcagaa agaacaaggg gcgatccgct
     2221 ccgccgctct cgaacgtgaa aatcgggcga tgaaaatgca gcgcaccttt aaccgctccg
     2281 gtattcgtcc actgcatcag aactgctcct ttgagaacta tcgcgttgag tgtgaagggc
     2341 agatgaatgc gttaagcaaa gcgcgccagt atgtcgaaga gtttgacggc aacatcgcca
     2401 gcttcatctt ttctggtaag ccaggaaccg gcaaaaacca tctggcggcg gcaatctgca
     2461 acgagctgct gctacgcggt aaatccgtat tgatcatcac cgtggccgat attatgtcgg
     2521 cgatgaaaga taccttcagg aatagcggta ccagcgaaga acaactgctt aacgatctga
     2581 gcaacgttga tctgctggtg atcgatgaga tcggcgtgca gaccgaatcg aaatacgaaa
     2641 aagtgatcat caaccagatc gtcgatcgcc gctcttcttc caaacgccca accgggatgc
     2701 tgaccaacag taatatggaa gagatgacca agttgctggg cgaacgcgtg atggaccgta
     2761 tgcgcctggg taacagtttg tgggtgatct tcaactggga tagctaccgt agccgggtaa
     2821 caggtaaaga gtattaatcc ccgtttctgg cggtatactg cgacacaatt ccaccttgag
     2881 taatgatgat gaaaactgtt aaacatcttc tgtgctgtgc cattgccgcc agcgcattaa
     2941 tttccaccgg ggtgcatgct gcgtcctgga aagatgcgct ctccagcgcc gccagcgaac
     3001 ttggcaacca aaacagcacg acacaggaag gcggttggtc gctcgcgtca ttaactaact
     3061 tgcttagcag cgggaaccag gccttaagcg cagataacat gaacaatgcc gcaggcattc
     3121 tgcaatactg cgcgaagcaa aagctggcgt cggtaaccga tgccgaaaac atcaagaacc
     3181 aggtgctgga aaagctgggc ctgaacagtg aagaacaaaa agaagacacc aactatctgg
     3241 atggcattca gggtttgctg aaaaccaaag atggtcagca actcaatctg gataacatcg
     3301 gaacgactcc gctggcagaa aaggtgaaaa ccaaagcctg cgatctggtg ttaaagcagg
     3361 ggctgaactt catttcctga accgcgaaac gccgcgtttt agctgttacc cggctgacgc
     3421 ggctctctcc tgcccttttc cctgctcctg gacggttttc cccctgttac acgggaagcg
     3481 accgcgatca aaaaagtctg tttataaact aattcctaac aacatcgcat ttttttgaca
     3541 ctagaattgc agcaatatgg cgacgccatt acattgtgct tctcaaaaag cttaaacgtt
     3601 ggctaaacgg cccggtttag tgaggatcgc ccgttgtcag aactactctc tttcgccctt
     3661 tttctcgcct ctgtgctgat ttacgcatgg aaagcgggac gtaacacctg gtggtttgca
     3721 gccacattaa cggtgctggg gctatttgtc gttttaaata tcaccctgtt tgccagcgac
     3781 tattttactg gcgatggtat taacgacgcg gttctctata ccttaaccaa tagcctgacc
     3841 ggtgctggcg tcagcaaata catcctgccg ggtatcggca ttgtgctggg gctgacggcg
     3901 gtgttcggtg cgctgggctg gatcctgcgc cgtcgtcgtc atcatccgca ccattttggt
     3961 tacagcctgc tggcgctctt actggcgctg ggttcggtgg acgccagccc ggcatttcgt
     4021 cagataacgg aactggtgaa atcccagtca cgcgacggcg acccggactt tgcggcttat
     4081 tataaagagc cgtcgaaaac tatccctgac ccgaaactca acctggttta tatctatggc
     4141 gaaagtctcg agcgaaccta ttttgataac gaagctttcc cggatctcac gcctgaactg
     4201 ggcgcgttga aaaatgaagg cctggatttc agccacacgc agcagctgcc aggaacggat
     4261 tacacgattg cgggcatggt ggcttctcag tgcggcatac cgctgtttgc cccctttgaa
     4321 ggcaacgcct ccgcttctgt ttccagcttc ttcccgcaga acatctgtct gggcgatatc
     4381 ctgaaaaact cgggttatca gaactatttc gtgcagggcg cgaatctgcg ttttgccggt
     4441 aaagatgtgt tcctgaagtc gcacggtttc gaccatttat acggctcaga agagctgaaa
     4501 agcgtggtgg ccgacccgca ctatcgcaac gactggggat tctacgacga taccgttctc
     4561 gatgaagcgt ggaaaaagtt tgaagagctt tcccgttctg gtcagcgatt ctcactgttt
     4621 accctgacag tcgataccca tcacccggat ggttttatct cgcgtacctg taaccgcaaa
     4681 aaatatgatt ttgacggtaa gccgaaccag tcattcagcg cggtaagttg cagccaggag
     4741 aatatcgcgg cgtttatcaa caaaatcaaa gcgtcaccgt ggtttaaaga taccgtcatc
     4801 gtcgtctctt ctgaccattt agcgatgaac aacacggcgt ggaaatacct caataagcag
     4861 gatcgcaata acctgttttt tgtcattcgt ggcgacaagc cgcagcaaga gacgctggca
     4921 gtgaagcgta acacgatgga taacggcgcg acggtgctgg acattctcgg tggcgataac
     4981 tatctcggac ttggtcgtag cagtttatcc gggcagtcga tgtcggaaat cttcctcaat
     5041 atcaaagaga aaacattggc gtggaagcca gatatcatcc gcctgtggaa attccctaaa
     5101 gagatgaaag agttcaccat cgaccagcag aaaaacatga ttgccttctc gggtagccat
     5161 ttccgtttgc cgctgctgtt gcgggtttca gacaaacgcg tggaaccgct gccggaaagc
     5221 gaatactcag caccgctgcg tttccagctg gccgatttcg ctccacgcga caatttcgtc
     5281 tgggttgacc gttgctataa gatggcacaa ctctgggctc cggaactggc actttccacc
     5341 gactggtgtg tctcgcaggg gcagcttggc ggtcagcaaa ttgtccagca tgttgacaaa
     5401 acaacatgga agagcaagac ggcatttaaa gatacggtca tcgacatggc gcgttacaaa
     5461 ggcaatgtcg atacgctgaa gattgttgat aacgatattc gctacaaagc cgacagtttc
     5521 atctttaacg tcgccggtgc gccagaagag gtgaaacagt ttagcgggat ttcccgtcca
     5581 gagtcgtggg gccgctggtc caacgcgcag ctgggcgatg aagtaaaaat cgagtacaag
     5641 catccgctgc cgaagaaatt tgacctggtg attaccgcca aagcatacgg caataacgcc
     5701 agccgtccta ttccggtacg cgtaggcaat gaagaacaaa ctcttgtgct gggcaatgaa
     5761 gtgaccacca ccacactgca tttcgataac ccaaccgatg ccgacacact ggtaattgtg
     5821 ccgccggaac ctgtctcaac caacgaaggg aatatcctcg gacactcgcc gcgtaagctc
     5881 gggatcggca tggtggaaat taaagttgtg gaacgtgaag ggtaattcct gactgatgtg
     5941 taggtcgcat aaggtgcgac ctacacacct tctccggcct acaaaacatc attgagaaac
     6001 agaaaaaaaa cagcgcccaa cacttccccg gacgctgcat ggcaacttta ataatctcgc
     6061 cttatcagaa agtaattacg cctttaatta actcacgatt gttaatcaca tcgcgctcgt
     6121 aggtttctgc cagcgtggcg aacggatagc gatgggttaa catcatgtcg gcagtgattt
     6181 tcccttccgc cataagtcga ccgactttag cgaaatcttc cggcgtggcg ttgcggctgc
     6241 ccatcatcgt cgtttctttt ttatggaatt ccggatcgga gaactgcaac tcacctttaa
     6301 acaggccgac aaataccacc gtgccgccgt gacgaatcaa attcacggtg ttattcatcg
     6361 catgttgatt acctgtcgcg tcgatcactt tctgcgccag cgaaccacca aactgcgccc
     6421 gcagctgcgc gtcaaaatct tcggctgacg ggtccagcac gggcaactcc agacgcgttg
     6481 ccacatgttc acggcgcgcc ggactggtat ccgccaccac cacctgtgcg ccatcggctt
     6541 tagcgattgc cgccgcgccc agaccgattg gccccgcccc gaccaccagc acctgctcgc
     6601 cgggagcaat ggctgcgcga cgcaccgcat gagcgctaat agcgaaaggt tcaatcaatg
     6661 cggccgcctg cgggtcaata ccgtctgcgg gcaaaatgtt cgccatcggc acgctcaaat
     6721 actcactaaa accgccatcc tgatgcacgc caatgactga aattttttca cagcaattgg
     6781 tacgcccgct tttacacgcc gggcattgct gacaggcaac ataagggatc acagcaactt
     6841 gctgaccatt tttaagatca gcaatatttt tacccagccc aacaatctcc ccacatattt
     6901 catggcctaa aacacgtgga taactaaaaa atggttgatt tccaccccag gcatgaatat
     6961 cggtaccgca aatcccgaca gacttaattt ttattaatgc ttcattgtca cccggaatag
     7021 gtatctcgcg ttgtttccag actaattctt tcggctgctg gcaaattaaa acattcatcg
     7081 tagacatgat tcggctctcc attttttgtt gccttagata tcaatgaaaa tgaaaaatgg
     7141 cagcggaacg gaaaatctgt tttgtgaaaa cacgcgcata aaaatgtttt aaatcgggtt
     7201 ttaatggact cacaaggagg aaaatatgag tcgttcacaa aatttacgcc acaatgtgat
     7261 taaccaggtc attgatgata tggcgcgggg tcatatccct tccccacttc catcgcaaag
     7321 tgcgctggcg gaaatgtaca acatcagccg cactacagtg cgtcatattc tcagccactt
     7381 acgcgaatgc ggcgtcttaa cgcaggttgg aaacgactat gttattgcgc gtaaacctga
     7441 ccatgatgac ggtttcgcct gtactaccgc ttcaatgagt gaacaaaaca aagtgtttga
     7501 gcaggctttt ttcacgatga taaaccagcg ccagttacgc ccaggggaaa ctttttctga
     7561 actgcaactg gctcgggccg cgggtgtcag cccggtggtt gtaagagaat atcttttaaa
     7621 attcggccgt tataatctta ttcaaagcga aaagcgcggc cagtggagca tgaagcaatt
     7681 cgatcaatcc tacgccgagc agctgtttga gctacgcgaa atgctcgaaa cacattcact
     7741 tcagcacttt ctcaacctgc ctgatcatga tccccgctgg ctgcaggcga aaaccatgct
     7801 cgaacgtcat cggctactgc gtgacaacat cggcaatagc tttcgcatgt tctcgcaact
     7861 tgacagagat tttcactcct tactgctttc agctgccgac aatatctttt ttgatcaatc
     7921 tcttgagatc atctcagtca tctttcattt tcactatcaa tgggacgaaa gcgatcttaa
     7981 acagcgcaat atcattgccg tcgatgagca tatgaccatt ctcagcgctc tgatttgccg
     8041 cagtgacctt gatgccaccc ttgcgctccg taaccatttg aattcagcta aacaatcaat
     8101 gattcgatcc attaatgaga acacgcgtta tgctcattaa gtacggatta aaaacagcaa
     8161 actattagcg ggtgcacggt tttatcaaaa gataaatacc gggaaattcc cgcttaccta
     8221 tgctcacaat cagtcgacta aaaatgtttt tgtgagcggt agtaaagtcc taaaacttta
     8281 acctgccgtt aatgtcaacc ttgcgtaatg cacaattacg taaacaatta aaattataaa
     8341 tacaggagtc cggcgtggaa aaagaaaata tcaccctcga tccgcgttct tcatttactc
     8401 catcttcgtc ggcagatatt cccgtgccac cagatggatt agttcaacgc agtacccgaa
     8461 ttaaacgcat tcaaaccacc gccatgttgt tattattttt tgcggcggta atcaattatc
     8521 tcgaccgcag ttcgctgtcg gtagcaaatt taacgattcg tgaagaattg ggattaagtg
     8581 ccaccgaaat cggcgctttg ctctccgtgt tttcactcgc ttacgggatt gcgcaacttc
     8641 cttgcggccc actattggat cgtaaaggcc cacgcctgat gctgggactg gggatgttct
     8701 tctggtcact gttccaggcc atgtctggca tggtgcacag ctttacgcag ttcgtgttgg
     8761 tgcgtatcgg tatggggatt ggtgaagcgc cgatgaaccc atgtggcgta aaagtcatta
     8821 acgactggtt caacatcaaa gagcgcggac gcccgatggg cttcttcaac gcagcttcta
     8881 ccattggcgt tgccgtaagc ccaccgattc tggcggcgat gatgctggtg atgggctggc
     8941 gtgggatgtt tattaccatt ggtgtgctgg ggatttttct cgccattggc tggtatatgc
     9001 tctatcgcaa ccgcgagcac gtagaactga ctgccgttga acaagcttat ctcaatgcag
     9061 gtagcgtcaa tgcccgccga gatccgctca gttttgccga atggcgcagc ctgttccgta
     9121 accgcacaat gtggggaatg atgctcggat tcagtggcat caactacact gcgtggctgt
     9181 atctggcctg gcttcctggt tacctgcaaa cagcctataa cctggattta aaaagcacag
     9241 ggttgatggc ggctatccct ttcctgtttg gggctgccgg gatgctggtc aacggttacg
     9301 ttactgactg gctggtcaaa gggggaatgg ctccgattaa aagccgtaag atctgcatta
     9361 ttgccgggat gttctgttct gccgccttta cgctggtcgt accgcaagcg acaacatcca
     9421 tgacggcggt tctgctgatt ggcatggcac tgttctgtat tcactttgcc ggaacatcct
     9481 gctggggctt gatccacgtc gcagttgctt ctcgcatgac ggcgtcggtg ggcagtatcc
     9541 agaactttgc cagcttcatc tgcgcctctt ttgcgccgat cattactggt tttattgttg
     9601 ataccaccca ctcattccgt ctggcactaa tcatctgcgg ttgcgtcacc gcagcgggag
     9661 cactggcgta catcttcctg gttcgtcagc cgatcaacga cccgcgtaaa gattaaactc
     9721 cctatgcccg ataacatttt gcttatcggg catttttatg gcgattaaaa cgtttcccag
     9781 ttctccccgc tatctgccac tgccatttta cgcggcgtag ctggcgtcac gttttttacc
     9841 acagccgatg tttcacgctg ctgttgctgc tgttgctgaa tccggaacac tgccacagct
     9901 tcggtcaggc gactggcctg ctcttccagc gcggcggcgg cagcggcaga ttcttccacc
     9961 agcgcagcgt tctgttgagt tacccggtcc atctcagcaa ccgctaagcc aacctgatcg
    10021 ataccacggc tctgctcatc agaagcagaa gcaatttcgc ccataatgtc cgtcacgcgg
    10081 gtcacggcac tgacaatctc cgccattgtt tcccctgcgc tttcgaccag cgtagagcca
    10141 acatctactt tccccaccga gtcttcaatc aggcttttaa tttcacgagc cgcctgagcg
    10201 ctgcgctgag ccagattacg cacttcaccc gcgaccaccg caaaaccgcg cccttgctca
    10261 cccgcgcgcg ctgcttcaac cgccgcgttc aaagccagaa tattggtctg gaaggcaatg
    10321 ccgtcaatta cgctgataat atcggcgatt ttctgcgaac tggtggagat atcgcgcatg
    10381 gtctgtacta cgttatccac cactttgccg ccgcgttgcg ccgtttcaga agcacttaac
    10441 gccagatggc tggcctgacg cgcattctcg gcgttctgtt tcaccgttgc ggtcagttgc
    10501 tccatgctgg ctgccgtctc ttccagcgaa gcggcctgtt gctcggtgcg cgaagagaga
    10561 tcgttattgc cggtggcgat ttcgctggca ccgctataga tggcattggc cccgttgcgc
    10621 acatcaccga cggtacgcat cagctctccc tgcatatggc gcaaactctc tgccagttgc
    10681 cccatctcat tagagccatc cacctcaatc ggtttcacca gatcgccgcc tgcaatatga
    10741 cgaatgctgt caatcaggcg attcattggc gctaccagcg aggctttaat accgaaccag
    10801 acggcgaaga tgaccgccag tacgacgatc atcacgccca ccagaatcca catcgcctgg
    10861 ctgtaggagg cattgttatc gctgacggcg atatcataga gccgatcgtc ttgctccatg
    10921 taagccacat actgcttctc gaaaccgtcc tgatatccct gggtcggctg atcaaagaac
    10981 tcgttgattt tgcctgcacc taatagttgg atcagctccg ccagcgcatt gtgataaata
    11041 tcgtaattac gtttgatctc tgccgctgcg gcggtgctct gacgcgggtc acgcggcaac
    11101 gcttcgtaat ccgcccagtt tttttccgcc tgtttcagcg aaatactggc actctgcatc
    11161 agctcagcaa cggttgaacc gctaccaata ttattctgat ccatcatgta gcggataccc
    11221 gcgcggttga gggtgttacg cgtctgcaac aacgcgaccc agctgccatt cagcgtggat
    11281 tgctgctggc gaatggtttg taaaacagtg aaattttctt tgtcattctt taaggcatta
    11341 aagaacagac cgcctgatgt cagttgtaaa aggccaaaaa cggccaaaac cagcagtaag
    11401 ctggtcacaa ttttgatacg ttttaacatg gtttctcttt cctgtggacc gatgcaagat
    11461 tttcggcctg gaaaggaaaa actttatgaa tatcccgcgg agattacgcc gagtaatttt
    11521 attcacactc tgaatttaaa aagccattat ttacataaat tattcacaat ataaatatgt
    11581 gatatgaatc acatatttat cgtcacttaa acgacgcctt tgccgctcaa ccgcaaaact
    11641 gaccgcttac atccctaaaa taaccactca gttatttacc ttactttacg cgcgcgtaac
    11701 tctggcaaca tcactacagg atagcggtca atttacctcc tcaaacgcaa cgcaaaccta
    11761 gaacggcttc ggccaactat taatcaatac atgccaggtt ttactatgga tactaaaaag
    11821 atattcaagc acataccctg ggtgattctc ggaatcatcg gtgcattctg cctcgcggta
    11881 gttgcattac gtcgggggga gcacgtcagc gccctgtgga tcgtggtcgc ctctgtatcg
    11941 gtgtatctgg tggcgtatcg ctactacagt ctgtacatcg cccagaaggt gatgaaactc
    12001 gaccccacgc gcgcgacgcc tgcggttatt aacaacgacg gtctgaacta cgttccgacc
    12061 aaccgttacg tgttgtttgg tcaccacttc gccgctatcg ccggtgctgg tccgctggtg
    12121 ggtccggttc tcgccgcgca gatgggctac ctgcctggca cgctgtggct gctggcgggg
    12181 gtcgtgctgg ccggtgcggt tcaggacttt atggtgctgt ttatctcctc tcgccgtaat
    12241 ggcgcatctc ttggtgagat gatcaaagaa gagatgggac cagtaccggg gactatcgcg
    12301 ctgtttggct gtttcttaat catgatcatc atcctcgccg tcctggcgct gattgtggtt
    12361 aaagccctgg ccgaaagtcc gtggggtgtc ttcaccgttt gctcaaccgt accgattgcg
    12421 ctgtttatgg gtatctacat gcgctttatt cgtccggggc gtgtgggtga agtctctgtc
    12481 attggtatcg tgctgctggt tgcctctatc tacttcggtg gcgtgattgc tcacgatccg
    12541 tactggggtc cggcactgac ctttaaagac accaccatta ccttcgcgct gattggctat
    12601 gcgtttgttt ccgcactgct gccggtgtgg ctgatcctcg caccgcgcga ctatctggca
    12661 accttcctga aaatcggcgt tatcgtcggc ctggcgctgg gtatcgtggt gctgaacccg
    12721 gaactgaaaa tgcctgccat gacccagtac attgacggta ctggcccgct gtggaaaggc
    12781 gctctgttcc cgttcctgtt catcaccatc gcctgtggtg cggtatctgg cttccacgcg
    12841 ctgatctctt ccggtacgac gccaaaactg ctggctaatg aaaccgacgc gcgtttcatc
    12901 ggctacggcg caatgctgat ggagtccttc gtggcgatta tggcgctggt tgctgcgtcc
    12961 atcatcgaac cgggtcttta cttcgcgatg aacaccccgc ctgctggcct tggcatcacc
    13021 atgcctaacc tgcatgaaat gggtggcgag aacgcgccga tcatcatggc gcagctgaaa
    13081 gacgttaccg cacacgcggc agcgaccgtc agctcctggg gcttcgtgat ttcgccagag
    13141 cagatcctgc aaaccgcgaa agacattggt gagccttctg tcctgaaccg tgcaggtggt
    13201 gcgccgacgc tggcggtagg tatcgctcac gtgttccaca aagtgctgcc gatggctgac
    13261 atgggcttct ggtatcactt cggtattctg ttcgaagcac tgttcatcct gaccgcgctg
    13321 gatgcgggta cccgttctgg ccgctttatg ctgcaagacc tgctgggtaa cttcatcccg
    13381 ttcctgaaaa aaaccgactc tctggttgcc ggtatcatcg gtactgcggg ctgtgtgggt
    13441 ctgtggggct acctgctgta tcagggcgtg gtcgatccgc tgggcggcgt taagagcctg
    13501 tggccgctgt tcggtatttc caaccagatg ctggcagccg tagcgctggt actgggcacc
    13561 gttgtgctga ttaagatgaa gcgcacccaa tacatctggg taactgttgt tccggctgta
    13621 tggctgctta tctgcaccac ctgggcgctg ggcctgaaac tgttcagcac caacccgcag
    13681 atggaaggct tcttctacat ggcaagccag tacaaagaga agattgctaa cggtactgac
    13741 ctgacggcgc agcagattgc caacatgaac cacatcgttg tgaacaacta caccaacgca
    13801 ggtctgagta ttctgttcct gattgtggtg tacagcatca tcttctacgg tttcaaaacc
    13861 tggcttgcgg tgcgtaacag cgacaaacgt actgacaaag aaacaccgta cgttccaatc
    13921 ccggaaggcg gcgtgaagat ctcttcgcac cactaaccgt gtttagcccc gcttcggcgg
    13981 ggctttgttc ttatcagagt gaactatgtt tggtaactta ggacaggcaa aaaaatatct
    14041 cggccaggcg gcgaagatgt tgattggcat tccagactac gacaactacg tcgagcatat
    14101 gaagaccaac catcccgaca agccgtacat gagctatgaa gaattcttcc gcgaacgcca
    14161 gaacgcacgc tacggcggcg atggtaaagg cggtatgcgc tgttgttaat ggagagagca
    14221 tgaacccgat tgcagttacc ctactcaccg gttttttagg cgcgggcaaa accaccctgc
    14281 tgcgccatat tcttaacgaa caacacggct acaagattgc cgtgattgaa aacgaattcg
    14341 gcgaagtctc cgttgatgat caactgattg gcgaccgcgc cacgcagatc aaaacgctga
    14401 ccaacggctg catctgctgt tcgcgctcca acgagctgga agacgcacta ctcgacctgc
    14461 tggataatct cgacaagggc aatattcagt tcgaccgtct ggtcattgaa tgcaccggca
    14521 tggccgatcc cggcccgatt attcagacct ttttctccca tgagatttta tgccagcgct
    14581 acctgctgga cggcgtgatt gcgctggtgg atgcggtaca tgccgatgag cagatgaacc
    14641 agttcaccat cgcccagtcc caggttggct acgccgaccg tattctgctg acgaaaaccg
    14701 acgtcgcagg cgaagcagag aaactgcgtg aacgcctggc gcgcatcaac gcccgcgcac
    14761 cggtctacac cgttacccac ggcgatatcg acctgggcct tctgttcaac accaacggtt
    14821 ttatgctgga agaaaacgtc gtcagcacca aaccgcgttt ccactttatc gcggacaaac
    14881 aaaacgatat ttcgtcgatt gtggtggaac tggattaccc ggtagatatc agcgaagttt
    14941 cccgggtgat ggaaaacctg ctgctggagt cggcggataa actactgcgt tacaaaggga
    15001 tgctatggat tgacggcgaa cctaaccgcc tgctattcca gggcgtccaa cgcctctaca
    15061 gcgccgactg ggaccgcccg tggggcgatg aaaaaccgca tagcacgatg gtgtttatcg
    15121 ggattcagtt gccggaggaa gagattcggg cagcgtttgc ggggttgagg aagtaatttt
    15181 ggatgatggc cgccatgtgg cggcctgaat tattttttgt tctgatcgcg cagcatcgct
    15241 tcacgtaaaa ttgcgttcag gcgagtttga tagcctttac ctggtcgctt caaccattcc
    15301 atcacatcag catcaatacg caccgaagcc tgagttttca atggccgata aaacttaccg
    15361 cgttcggcgt tagaccattg ctcatcgctg gaaggtggga tatcactgta atcaatatct
    15421 tcgtcggatt tattcgccag cgccttcagt tcggcctcat gctgcacatc gagtgtagag
    15481 gaggtccctc gtttatgttt aaccatgctc atagcgattc ctctctttac gatctgcttt
    15541 tcgtgcactg atgattcgaa taatttcatt tccgctttcg aaacggatgg tatgcgcaac
    15601 cagaataacc acgatgccgt aaaccaggcc gattgtctgc cagcgatact caccgttttc
    15661 tatgcgttct tgctgtgaca aatgttgtgg atcatcaaac aataacaccg catcttcaaa
    15721 gcggatgcca tgtttcaccc gattactttt ggccttgttt tcatcccatt caaactccat
    15781 tggcataatt gccatcccta attgtatata caaaaatgta tctacaatta ttctgcaatt
    15841 atagcgttga tgttggaaat agcatcacgt ttcgaataat caaccatgaa attgacaatg
    15901 agtaagttgg acttgattca tccaatagaa tttctcgcct gacatggcac actaaacacc
    15961 tcgctgtc
//
LOCUS       DAHHTG010000099        15871 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.099, whole genome shotgun sequence.
ACCESSION   DAHHTG010000099 DAHHTG010000000
VERSION     DAHHTG010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15871)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15871)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15871
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.099"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            complement(<1..710)
                     /gene="phoU"
                     /locus_tag="NBI74_003272"
     CDS             complement(<1..710)
                     /gene="phoU"
                     /locus_tag="NBI74_003272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HCD1933556.1"
                     /translation="MDSLNLNKHISGQFNAELESIRTQVMTMGGMVEQQLSDAITAMH
                     NQDSDLAKRVIEGDKNVNMMEVAIDEACVRIIAKRQPTASDLRLVMVISKTIAELERI
                     GDVADKICRTALEKFSQQHQPLLVSLESLGRHTIQMLHDVLDAFARMDIDEAVRIYRE
                     DKKVDQEYEGIVRQLMTYMMEDSRTIPSVLTALFCARSIERIGDRCQNICEFIFYYVK
                     GQDFRHVGGDELDKLLAE"
     gene            complement(725..1498)
                     /gene="pstB"
                     /locus_tag="NBI74_003273"
     CDS             complement(725..1498)
                     /gene="pstB"
                     /locus_tag="NBI74_003273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000063125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HCD1933557.1"
                     /translation="MSMVETAPSKIQVRNLNFYYGKFHALKNINLDIAKNQVTAFIGP
                     SGCGKSTLLRTFNKMFELYPEQRAEGEILLDGDNILTNSQDIALLRAKVGMVFQKPTP
                     FPMSIYDNIAFGVRLFEKLSRADMDERVQWALTKAALWNETKDKLHQSGYSLSGGQQQ
                     RLCIARGIAIRPEVLLLDEPCSALDPISTGRIEELITELKQDYTVVIVTHNMQQAARC
                     SDHTAFMYLGELIEFSNTDDLFTKPAKKQTEDYITGRYG"
     gene            complement(1589..2479)
                     /gene="pstA"
                     /locus_tag="NBI74_003274"
     CDS             complement(1589..2479)
                     /gene="pstA"
                     /locus_tag="NBI74_003274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HCD1933558.1"
                     /translation="MAMVEMQTTAALAESRRKMQARRRLKNRIALTLSMATMAFGLFW
                     LIWILMSTITRGIDGMSLALFTEMTPPPNTEGGGLANALAGSGLLILWATVFGTPLGI
                     MAGIYLAEYGRKSWLAEVIRFINDILLSAPSIVVGLFVYTIVVAQMEHFSGWAGVIAL
                     ALLQVPIVIRTTENMLKLVPDSLREAAYALGTPKWKMIASITLKASVSGIMTGILLAI
                     ARIAGETAPLLFTALSNQFWSTDMMQPIANLPVTIFKFAMSPFAEWQQLAWAGVLIIT
                     LCVLLLNILARVVFAKNKHG"
     gene            complement(2479..3438)
                     /gene="pstC"
                     /locus_tag="NBI74_003275"
     CDS             complement(2479..3438)
                     /gene="pstC"
                     /locus_tag="NBI74_003275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008806729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstC"
                     /protein_id="HCD1933559.1"
                     /translation="MAATKPAFNPPGKKGDIIFSVLVKLAALIVLLMLGGIIVSLIIS
                     SWPSIQKFGLAFLWTKEWDAPNDIYGALVPIYGTLVTSFIALLIAVPVSFGIALFLTE
                     LAPGWLKRPLGIAIELLAAIPSIVYGMWGLFIFAPLFAVYFQEPVGNIMSNIPIVGAL
                     FSGPAFGIGILAAGVILAIMIIPYIAAVMRDVFEQTPVMMKESAYGIGCTTWEVIWRI
                     VLPFTKNGVIGGIMLGLGRALGETMAVTFIIGNTYQLDSASLYMPGNSITSALANEFA
                     EAESGLHVAALMELGLILFVITFIVLAASKFMIMRLAKNEGAR"
     gene            complement(3524..4564)
                     /gene="pstS"
                     /locus_tag="NBI74_003276"
     CDS             complement(3524..4564)
                     /gene="pstS"
                     /locus_tag="NBI74_003276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HCD1933560.1"
                     /translation="MKVMRTTVATVVAATLSMSAFSVFAEASLTGAGATFPAPVYAKW
                     ADTYQKETGNKVNYQGIGSSGGVKQIIANTVDFGASDAPLSDEKLAQEGLFQFPTVIG
                     GVVLAVNIPGLKSGELVLDGKTLGDIYLGKIKKWDDEAIAKLNPGLKLPSQNIAVVRR
                     ADGSGTSFVFTSYLAKVNEEWKNNVGTGSTVKWPIGLGGKGNDGIAAFVQRLPGAIGY
                     VEYAYAKQNNLAYTKLISADGKPVSPTEENFANAAKGADWSKTFAQDLTNQKGEDAWP
                     ITSTTFILIHKDQKKPEQGTEVLKFFDWAYKTGAKQANDLDYASLPDSVVEQVRAAWK
                     TNIKDSSGKPLY"
     gene            complement(4878..6707)
                     /gene="glmS"
                     /locus_tag="NBI74_003277"
     CDS             complement(4878..6707)
                     /gene="glmS"
                     /locus_tag="NBI74_003277"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HCD1933561.1"
                     /translation="MCGIVGAIAQRDVAEILLEGLRRLEYRGYDSAGLAVVDAEGHMT
                     RLRRLGKVQMLAQAAEEHPLHGGTGIAHTRWATHGEPSEANAHPHVSEHIVVVHNGII
                     ENHEPLREELKARGYTFVSETDTEVIAHLVNWELKQGGTLREAVLRAIPQLRGAYGTV
                     IMDSRHPDTLLAARSGSPLVIGLGMGENFIASDQLALLPVTRRFIFLEEGDIAEITRR
                     SVNIFDKTGAEVKRQDIESNLQYDAGDKGIYRHYMQKEIYEQPNAIKNTLTGRISHGQ
                     VDLSELGPNADELLSKVEHIQILACGTSYNSGMVSRYWFESLAGIPCDVEIASEFRYR
                     KSAVRRNSLMITLSQSGETADTLAGLRLSKELGYLGSLAICNVPGSSLVRESDLALMT
                     NAGTEIGVASTKAFTTQLTVLLMLVAKLSRLKGLDASIEHDIVHGLQALPSRIEQMLS
                     QDKRIEALAEDFSDKHHALFLGRGDQYPIALEGALKLKEISYIHAEAYAAGELKHGPL
                     ALIDADMPVIVVAPNNELLEKLKSNIEEVRARGGQLYVFADQDAGFVSSDNMHIIEMP
                     HVEEVIAPIFYTVPLQLLAYHVALIKGTDVDQPRNLAKSVTVE"
     gene            complement(6869..8239)
                     /gene="glmU"
                     /locus_tag="NBI74_003278"
     CDS             complement(6869..8239)
                     /gene="glmU"
                     /locus_tag="NBI74_003278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HCD1933562.1"
                     /translation="MLNNAMSVVILAAGKGTRMYSDLPKVLHTLAGKAMVQHVIDAAN
                     ELGAAHVHLVYGHGGDLLKQALKDDNLNWVLQAEQLGTGHAMQQAAPFFADDEDILML
                     YGDVPLISVETLQHLRDAKPQGGIGLLTVKLDDPTGYGRITRENGKVTGIVEHKDATD
                     EQRQIQEINTGILIANGADMKRWLAKLTNNNAQGEYYITDIIALAYQEGREIVAVHPQ
                     RLSEVEGVNNRLQLSRLERVYQSEQAEKLLLAGVMLRDPARFDLRGTLTHGRDVEIDT
                     NVIIEGNVTLGHRVKIGTGCVIKNSVIGDDCEISPYTVVEDANLAAACTIGPFARLRP
                     GAELLEGAHVGNFVEMKKARLGKGSKAGHLTYLGDAEIGDNVNIGAGTITCNYDGANK
                     FKTIIGDDVFVGSDTQLVAPVTVGKGATIAAGTTVTRNVGENALAISRVPQTQKEGWR
                     RPVKKK"
     gene            complement(8593..9012)
                     /gene="atpC"
                     /locus_tag="NBI74_003279"
     CDS             complement(8593..9012)
                     /gene="atpC"
                     /locus_tag="NBI74_003279"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001251972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HCD1933563.1"
                     /translation="MAMTYHLDVVSAEQQMFSGLVEKIQVTGSEGELGIYPGHAPLLT
                     AIKPGMIRIVKQHGHEEFIYLSGGILEVQPGNVTVLADTAIRGQDLDEARAMEAKRKA
                     EEHISSSHGDVDYAQASAELAKAIAQLRVIELTKKAM"
     gene            complement(9033..10415)
                     /gene="atpD"
                     /locus_tag="NBI74_003280"
     CDS             complement(9033..10415)
                     /gene="atpD"
                     /locus_tag="NBI74_003280"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017800291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HCD1933564.1"
                     /translation="MATGKIVQVIGAVVDVEFPQDAVPRVYDALEVQNGNERLVLEVQ
                     QQLGGGIVRTIAMGSSDGLRRGLDVKDLEHPIEVPVGKATLGRIMNVLGEPVDMKGEI
                     GEEERWAIHRAAPSYEELSNSQELLETGIKVIDLMCPFAKGGKVGLFGGAGVGKTVNM
                     MELIRNIAIEHSGYSVFAGVGERTREGNDFYHEMTDSNVIDKVSLVYGQMNEPPGNRL
                     RVALTGLTMAEKFRDEGRDVLLFVDNIYRYTLAGTEVSALLGRMPSAVGYQPTLAEEM
                     GVLQERITSTKTGSITSVQAVYVPADDLTDPSPATTFAHLDATVVLSRQIASLGIYPA
                     VDPLDSTSRQLDPLVVGQEHYDTARGVQSILQRYQELKDIIAILGMDELSEEDKLVVA
                     RARKIQRFLSQPFFVAEVFTGSPGKYVSLKDTIRGFKGIMEGEYDHLPEQAFYMVGSI
                     EEAVEKAKKL"
     gene            complement(10442..11305)
                     /gene="atpG"
                     /locus_tag="NBI74_003281"
     CDS             complement(10442..11305)
                     /gene="atpG"
                     /locus_tag="NBI74_003281"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HCD1933565.1"
                     /translation="MAGAKEIRSKIASVQNTQKITKAMEMVAASKMRKSQDRMAASRP
                     YAETMRKVIGHLAHGNLEYKHPYLEDRDVKRVGYLVVSTDRGLCGGLNINLFKKLLAE
                     MKTWTDKGVQCDLAMIGSKGVSFFNSVGGNVVAQVTGMGDNPSLSELIGPVKVMLQAY
                     DEGRLDKLYIVSNKFINTMSQVPTISQLLPLPASDDDDLKHKSWDYLYEPDPKALLDT
                     LLRRYVESQVYQGVVENLASEQAARMVAMKAATDNGGSLIKELQLVYNKARQASITQE
                     LTEIVSGAAAV"
     gene            complement(11356..12897)
                     /gene="atpA"
                     /locus_tag="NBI74_003282"
     CDS             complement(11356..12897)
                     /gene="atpA"
                     /locus_tag="NBI74_003282"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HCD1933566.1"
                     /translation="MQLNSTEISELIKQRIAQFNVVSEAHNEGTIVSVSDGVIRIHGL
                     ADCMQGEMISLPGNRYAIALNLERDSVGAVVMGPYADLAEGMKVKCTGRILEVPVGRG
                     LLGRVVNTLGAPIDGKGPLDHDGFSAVEAIAPGVIERQSVDQPVQTGYKAVDSMIPIG
                     RGQRELIIGDRQTGKTALAIDAIINQRDSGIKCIYVAIGQKASTISNVVRKLEEHGAL
                     ANTIVVVATASESAALQYLAPYAGCAMGEYFRDRGEDALIIYDDLSKQAVAYRQISLL
                     LRRPPGREAFPGDVFYLHSRLLERAARVNAEYVEAFTKGEVKGKTGSLTALPIIETQA
                     GDVSAFVPTNVISITDGQIFLETNLFNAGIRPAVNPGISVSRVGGAAQTKIMKKLSGG
                     IRTALAQYRELAAFSQFASDLDDATRKQLDHGQKVTELLKQKQYAPMSVAQQSLVLFA
                     AERGYLADVELSKIGSFEAALLAYVDRDHAPLMQEINQTGGYNDEIEGKLKGILDSFK
                     ATQSW"
     gene            complement(12910..13443)
                     /gene="atpH"
                     /locus_tag="NBI74_003283"
     CDS             complement(12910..13443)
                     /gene="atpH"
                     /locus_tag="NBI74_003283"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HCD1933567.1"
                     /translation="MSEFITVARPYAKAAFDFAVEHQSVERWQDMLAFAAEVTKNEQM
                     AELLSGALAPETLAESFIAVCGEQLDENGQNLIRVMAENGRLKALPDVLEQFIHLRAV
                     SEATTEVDVISAAALSEQQLAKISAAMEKRLSRKVKLNCKIDKSVMAGVIIRAGDMVI
                     DGSVRGRLERLADVLQS"
     gene            complement(13458..13928)
                     /gene="atpF"
                     /locus_tag="NBI74_003284"
     CDS             complement(13458..13928)
                     /gene="atpF"
                     /locus_tag="NBI74_003284"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001052215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HCD1933568.1"
                     /translation="MNLNATILGQAIAFVLFVLFCMKYVWPPLMAAIEKRQKEIADGL
                     ASAERAHKDLDLAKASATDQLKKAKAEAQVIIEQANKRRSQILDEAKAEAEQERTKIV
                     AQAQAEIEAERKRAREELRKQVAILAVAGAEKIIERSVDEAANSDIVDKLVAEL"
     gene            complement(13990..14229)
                     /gene="atpE"
                     /locus_tag="NBI74_003285"
     CDS             complement(13990..14229)
                     /gene="atpE"
                     /locus_tag="NBI74_003285"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004246596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HCD1933569.1"
                     /translation="MENLNMDLLYMAAAVMMGLAAIGAAIGIGILGGKFLEGAARQPD
                     LIPLLRTQFFIVMGLVDAIPMIAVGLGLYVMFAVA"
     gene            complement(14276..15091)
                     /gene="atpB"
                     /locus_tag="NBI74_003286"
     CDS             complement(14276..15091)
                     /gene="atpB"
                     /locus_tag="NBI74_003286"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HCD1933570.1"
                     /translation="MASENMTPQDYIGHHLNNLQLDLRTFSLVDPHNPPATFWTINID
                     SMFFSVVLGLLFLVLFRSVAKKATSGVPGKFQTAIELVIGFVNGSVKDMYHGKSKLIA
                     PLALTIFVWVFLMNLMDLLPIDLLPYIAEHVLGLPALRVVPSADVNVTLSMALGVFIL
                     ILFYSIKMKGIGGFTKELTLQPFNHWAFIPVNLILEGVSLLSKPVSLGLRLFGNMYAG
                     ELIFILIAGLLPWWSQWILNVPWAIFHILIITLQAFIFMVLTIVYLSMASEEH"
     gene            complement(15100..15480)
                     /gene="atpI"
                     /locus_tag="NBI74_003287"
     CDS             complement(15100..15480)
                     /gene="atpI"
                     /locus_tag="NBI74_003287"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418195.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit I"
                     /protein_id="HCD1933571.1"
                     /translation="MSVSLVSRNVARKLLLVQLLVVIASGLLFSLKDPFWGVSAISGG
                     LAVFLPNVLFMIFAWRHQAHTPAKGRVAWTFAFGEAFKVLAMLVLLVVALAVLKAVFL
                     PLIVTWVLVLVVQILAPAVINNKG"
BASE COUNT         3822 a         4320 c         3955 g         3774 t
ORIGIN      
        1 ttttctgcca gcagtttatc gagctcgtca ccaccgacgt gacggaaatc ctgccccttc
       61 acgtagtaga agataaactc acaaatattc tggcagcggt cgccaatacg ttcgatagaa
      121 cgcgcacaga ataacgcggt aagtacgctc ggaatggtac gcgaatcttc catcatgtag
      181 gtcatcagtt gacgaacaat accttcgtac tcctgatcga cttttttatc ttcacgataa
      241 atacgtaccg cttcgtcaat gtccatccgc gcgaacgcgt ccagcacgtc gtgcagcatc
      301 tggatggtat gacggcccag cgactccaga cttaccagca acggctgatg ctgctgggag
      361 aatttctcca gcgcagtacg gcagatttta tccgccacgt caccaatacg ctccagctcg
      421 gcaatggttt tactgattac catcaccaga cgcaggtcgc tcgccgtcgg ctgacgtttg
      481 gcgataatgc gcacgcaggc ttcatcgatc gccacttcca tcatgttgac gttcttgtcg
      541 ccttcgatga cgcgcttcgc cagatcgctg tcctggttat gcatcgcggt gattgcatca
      601 gaaagctgct gctccaccat gccgcccatg gtcatcacct gcgtgcggat gctttccagt
      661 tcggcgttga actggccgga aatatgttta ttaagattga gactgtccat aacgcactcc
      721 tgaatcaacc gtaacgaccg gtgatgtagt cttctgtttg ttttttcgct ggcttggtga
      781 acagatcgtc cgtgttgctg aactcaatca attcgcccag gtacataaac gccgtgtggt
      841 cggaacaacg cgcagcctgc tgcatgttgt gggtgacgat caccacggtg taatcctgct
      901 tcagttcggt gatcagctct tcaatacgcc cggtagagat agggtcgagt gccgaacacg
      961 gttcgtcgag cagcagcact tccgggcgaa tggcgatacc acgcgcaatg cacagacgct
     1021 gttgctgacc accagagaga gagtaaccgc tctggtgcaa tttatctttg gtttcgttcc
     1081 acaatgcggc tttggtcaat gcccactgca cgcgctcgtc catgtcggca cgggagagct
     1141 tctcaaacag acgcacgcca aaggcgatgt tgtcgtaaat ggacatcggg aacggcgtcg
     1201 gtttctggaa caccatgccc actttcgcac gcagcagggc gatatcctga gagttggtca
     1261 ggatgttgtc gccatcgagc agaatttcac cttccgcacg ctgctccggg tacagttcaa
     1321 acattttgtt gaaggtacgc agcagcgttg atttaccgca gccggacggc ccgataaacg
     1381 ccgtaacctg gtttttggcg atatccaggt tgatgttttt cagggcatgg aatttgccgt
     1441 agtagaagtt caaattacga acctgaattt tactcggggc agtttcaacc atactcattg
     1501 caatcttttc ctcatcgtgc ctgatgcact tcgcgaatca ggcttaccgt aggccggata
     1561 aggcgtttac gccgcatcca gcaaagtgtt aaccgtgttt attcttcgca aaaacaacgc
     1621 gcgccagaat gttcagcagc agtacgcaca gggtaatgat caacaccccg gcccaggcca
     1681 gttgctgcca ttccgcaaac gggctcatcg caaacttaaa gatcgtcacc ggcaggttgg
     1741 cgatcggctg catcatgtcc gtgctccaga actggttgga gagcgcagta aacagcagcg
     1801 gtgcggtttc accagcaata cgggcaatcg ccagcaggat accggtcatg atcccggaca
     1861 ccgacgcttt cagcgtgatc gaggcgatca tcttccactt cggcgtaccc agcgcataag
     1921 ccgcttcacg caggctgtcc ggcaccagtt tcagcatgtt ctcggtggtg cggataacaa
     1981 tcggcacctg caacagcgcc agcgcaatca cgcccgccca gccggagaag tgctccatct
     2041 gcgccaccac gatggtgtaa acaaaaagac caaccacaat cgacggcgcg gagagcagaa
     2101 tgtcgttaat gaagcgaatc acttctgcca gccaggattt acgaccatat tcagccaggt
     2161 aaatccccgc cataatgccc agcggcgtac cgaataccgt agcccacaaa attaacaggc
     2221 cgctacccgc cagggcgttc gccagaccac cgccttccgt attgggtggt ggcgtcattt
     2281 cggtgaacag cgccagcgac ataccgtcga taccgcgagt gatggtggac attaaaatcc
     2341 agatcagcca gaacagaccg aaggccatcg tcgccattga gagcgtcagc gcaatacggt
     2401 ttttgaggcg gcgacgcgcc tgcattttgc ggcgggattc agccagtgcc gcagtggttt
     2461 gcatttcaac catagccatt agcgtgcccc ctcattctta gccaggcgca taatcataaa
     2521 cttcgatgcg gcgaggacga tgaaggtaat cacaaacagg atcaggccca gttccatcag
     2581 tgcagcaacg tgcagacccg actctgcttc ggcaaattcg ttcgccagcg cagaggtgat
     2641 actgttgccc ggcatgtaca gcgaggcgct gtcgagctgg taggtgttac cgatgataaa
     2701 ggtcaccgcc atggtttcac caagcgcgcg gcccagcccc aacataatgc cgccgataac
     2761 accatttttg gtgaacggaa gaacgatacg ccagataact tcccaggtgg tgcagccaat
     2821 accgtaggcc gactctttca tcatcaccgg ggtttgttcg aacacatcgc gcattaccgc
     2881 cgcaatgtac ggaataatca tgatggcgag gatcacgcct gccgcgagga taccgatacc
     2941 aaaggccggg ccggagaaca gcgcgccgac aatcgggata ttcgacatga tattgccgac
     3001 cggctcctgg aagtaaacgg cgaacagcgg cgcaaagata aacaggcccc acatgccgta
     3061 aacgatactt ggaatggctg ctagcagctc aatggcgata cccagcgggc gcttcagcca
     3121 gccaggcgca agctcggtca ggaacagggc gataccgaaa ctcaccggga cggcgatcag
     3181 cagcgcgata aacgaggtca ccagcgtacc gtagatcggc accagcgccc cgtagatatc
     3241 gttcggcgca tcccactctt tggtccacag gaaagccaga ccaaatttct gaatgctcgg
     3301 ccaggaggag atgatcagag agacaataat gccacccaac atcaatagca caatcagcgc
     3361 cgccagtttt accagcacgc tgaaaattat gtcgcccttt ttacccggtg ggttaaaagc
     3421 aggcttggtt gcagccataa gttactcttc agttaaacgc gtttacaaag tcattttgta
     3481 atgccggatg cggcgtaaaa caccgtaccc ggcctgagtt ttattagtac agcggcttgc
     3541 cactactgtc tttaatattg gtcttccacg cagcgcgaac ctgttccact acgctatccg
     3601 gcaggctggc gtaatccagg tcgttcgcct gtttagcccc ggttttgtat gcccagtcga
     3661 agaacttcag cacttctgtg ccttgttctg gtttcttctg atctttgtgg atcagaatga
     3721 acgtggtaga ggtaataggc cacgcatctt cgcctttctg gttggtcaga tcctgagcga
     3781 aggttttgct ccagtctgcg ccttttgctg cattagcgaa gttttcttcg gtcggactaa
     3841 ccggtttacc atcagcggag atcaatttgg tatacgccag gttgttctgc ttagcgtaag
     3901 catattcaac gtaaccaatt gcacccggca gacgctgaac gaacgcagcg ataccgtcgt
     3961 tacctttacc acccagaccg atcggccatt ttacggtaga accagtacca acgttgtttt
     4021 tccactcttc gttcactttc gccaggtagc tggtgaagac gaaggaagtc ccggagccat
     4081 ctgcgcggcg cactacagcg atgttctgtg acggcagctt cagacccgga ttcaatttgg
     4141 cgatggcttc atcatcccac tttttgattt tgcccaggta gatgtcgccg agggttttac
     4201 catccagcac cagctcacca gacttcagcc ccggaatgtt aaccgccagc accacgccgc
     4261 caatcacggt cgggaactgg aacagacctt cctgagccag tttttcgtca gacagcggcg
     4321 cgtcagaggc accgaaatca acggtgttag cgataatctg ttttacgcca ccggaagaac
     4381 cgataccctg gtagttaact ttattaccgg tttctttctg gtaagtgtca gcccatttgg
     4441 catacaccgg cgcagggaag gttgcacctg cacctgtcag gcttgcttct gcaaacacag
     4501 agaaagcgct catcgataag gtcgcggcga caacagttgc gacggtggta cgcataactt
     4561 tcataatgtc tcctgggagg attcataaag cattgtttgt tggctacgag aagcaaaata
     4621 ggacaaacag gtgacagtta tatgtaaggt ttatgacagt tttatgacag agagagaatg
     4681 tcttcagtct gatttaaata agcgttgata ttcagttaat tacaaatatt aataacgaag
     4741 agatgacaga aaaattttca ttctgtgtca gagaaaaagt agccgaagat gacggtttgt
     4801 cacatggagt tggcaggata tttgattaaa aacataacag gaagaaaaat gccctgctta
     4861 cgcagggcat ccatttatta ctcaaccgta accgatttcg ccaggttacg cggctggtca
     4921 acgtctgtgc ctttgatcag cgcaacgtgg taagccagta gttgcagcgg aacggtgtag
     4981 aagatcggtg caatcacctc ttccacatgc ggcatctcaa taatgtgcat attgtcactg
     5041 ctcacaaaac ccgcatcctg atcggcgaag acatacagct gaccgccacg cgcgcgaact
     5101 tcttcaatgt tggatttcag tttttccagc aattcgttgt tcggtgcaac gacgataacc
     5161 ggcatatcgg catcaatcag cgccagcgga ccgtgtttca gttcgcctgc agcgtaggct
     5221 tcagcgtgaa tgtaagagat ctctttcagc ttcaatgcac cttccagcgc gattgggtac
     5281 tgatcgccac ggcccaggaa cagcgcgtga tgtttgtcag agaaatcttc tgccagcgct
     5341 tcaatgcgtt tgtcctgaga cagcatctgc tcaatacggc tcggcagcgc ctgcagacca
     5401 tgcacgatgt catgttcaat ggaggcatcc agacctttca ggcgagacag tttcgccacc
     5461 agcatcaaca gcacagttaa ctgggtggtg aatgctttgg tggaggccac gccaatttcc
     5521 gtaccggcgt tggtcatcag cgccagatcg gattcgcgca ccagagaaga acccggaacg
     5581 ttacagattg ccagcgaacc aaggtaaccc agctctttcg aaagacgcag gcccgccagg
     5641 gtatccgcgg tttcgccaga ctgtgacaag gtgatcatca ggctgttacg acgcacggca
     5701 gatttgcgat agcggaattc agaggcgatt tcgacgtcgc acggaatacc tgccagcgat
     5761 tcaaaccagt agcgggaaac cataccggag ttataagaag taccacaggc gaggatctga
     5821 atatgctcaa ccttcgacag cagttcatcg gcgttcggtc ccagctcgct taaatcaacc
     5881 tgaccgtggc tgatgcgccc ggtaagggtg tttttgatcg cgttcggctg ttcgtagatc
     5941 tctttctgca tgtagtggcg gtaaatacct ttatcgcccg cgtcatattg cagattggat
     6001 tcgatatcct gacgttttac ttccgcgcca gttttatcga agatgtttac cgaacggcga
     6061 gtgatttccg caatatcgcc ctcttcaagg aagataaaac gacgagtcac tggcaacagc
     6121 gccagctggt cagaagcgat aaagttttcg cccataccca ggccaatcac cagcggacta
     6181 ccagaacgtg ccgccagcag ggtatccgga tgacgggagt ccatgatcac tgtaccgtac
     6241 gcaccacgca gttgcgggat agcacgcaga actgcctcac gcagagtacc gccttgtttc
     6301 agctcccagt tcaccaaatg tgcaatcact tcggtgtcgg tttcagaaac gaaggtatag
     6361 ccacgcgctt ttaactcttc acgcagcggt tcatggtttt cgatgatgcc gttatgcacc
     6421 accacaatgt gttcagaaac atgcggatgc gcgttagctt ctgaaggttc accgtgggtc
     6481 gcccagcgag tatgagcaat accggtgccg ccatgcagag gatgttcttc cgctgcctga
     6541 gccagcatct gtactttacc gaggcgacgc aggcgggtca tatgaccttc agcatccacc
     6601 acggccaggc cggcagagtc atatccgcgg tattccagac gacgtaaacc ttcaagaagg
     6661 atttctgcta catcacgttg cgcgatcgcg ccaacaattc cacacatagt ttttgattcc
     6721 gatttatatc gttgtcggtc aacctgtatg cccgtttttt cgggcgcccc gagccttgta
     6781 gagagtgggg ttatttttat agttactgct tgtgggcggg agattatgtt atctcctcat
     6841 cctatgtgac cggattaccc ggccagaatt actttttctt taccggacga cgccagcctt
     6901 ctttttgagt ctgcggcaca cggctgatag ccagggcatt ttcgccgaca ttacgcgtca
     6961 cagttgtacc cgcagcaatg gtcgcgcctt tgcctactgt taccggggcc accagttgag
     7021 tgtcggaacc gacaaacaca tcgtcgccga taatggtctt aaatttattc gcaccatcgt
     7081 agttgcaggt aatggttccc gcaccgatgt taacgttatc gccaatttcc gcatcgccca
     7141 ggtaagtcag atgaccagct ttcgagcctt tacccagacg cgcttttttc atctcaacga
     7201 agttaccgac gtgtgcacct tccagcaact cagcaccagg acgcagacgg gcaaacgggc
     7261 caatagtaca ggccgctgcc agattcgcat cttccacgac ggtatatgga ctgatttcgc
     7321 aatcatcgcc aatcacgctg tttttaatca cgcaaccggt gccgattttc acgcgatgac
     7381 cgagagtcac gttgccctcg atgataacgt tagtatcaat ttcaacatcg cgcccgtgag
     7441 taagcgtacc gcgcagatca aaacgcgctg gatcgcgcag cataacgcct gctaacagca
     7501 gtttttcagc ctgttcggac tggtaaacgc gctccagtcg ggagagttgc aggcggttat
     7561 tcacgccttc tacttcgctt aaacgttgcg gatgaacggc gacgatttca cgtccttcct
     7621 gatacgccag cgcaataatg tcggtgatgt agtattcgcc ctgagcatta ttgttggtca
     7681 gcttcgccag ccagcgtttc atatctgcgc cgttggcaat cagaatgccg gtgttgatct
     7741 cctgaatctg acgctgctcg tcggtggcat ctttgtgctc aacaatgccg gtaactttgc
     7801 cgttttcacg ggtgatacgt ccataaccgg tcggatcatc cagttttacc gtcagcagac
     7861 caatgccacc ttgcggttta gcatcacgca ggtgctggag tgtttcgaca gagatcagcg
     7921 gcacgtcgcc gtagagcatt aaaatgtctt catcatcggc aaagaaaggt gcggcctgct
     7981 gcatcgcatg acccgtaccc agctgttctg cctgaagcac ccagttcagg ttgtcgtctt
     8041 tcagcgcctg cttaagcaga tcgccgccgt gaccgtacac aaggtgaacg tgcgctgcgc
     8101 ctaattcatt cgcagcatca atgacatgct gaaccatcgc tttcccggca agggtatgca
     8161 gcactttcgg aagatcggaa tacatgcgcg tgcctttgcc tgcggcaagg atcactacgc
     8221 tcatagcatt attcaacata cgcgtcctga ctgtaatttg agaacgaatt taaaccgctt
     8281 caccttgaaa aaactacatt tttttcatcg tgaaatggac agaggataaa ttgttcaata
     8341 acggattatc cccgctgacg aaagcgccat tttcgaccat cgtcacgcct tttgtctgca
     8401 gaaaattaag gcagtacctt catttaagcg tcaggtggat gtttttgctc ttatttcgat
     8461 caatgaataa acgggaaaac aggggtgttt tatatcatca ggatctatgt gaacgctttt
     8521 caggacgggt caatacgcgt aaaaaaaagc cagcctgttg ccagactggc ttttgtgctt
     8581 ttcaagccgg tgttacatcg cttttttggt caactcgata acgcgcagct gcgcgatcgc
     8641 tttggccagt tccgcagacg cctgagcgta atctacgtcg ccgtgagagc tgctaatgtg
     8701 ctcttcagcc ttacgtttcg cttccatggc tcgcgcttcg tcgagatcct ggccgcgaat
     8761 tgcggtgtcg gccagaacgg tcacgttgcc aggctgcact tcaagaatgc cgccagacag
     8821 atagataaac tcttcgtgac cgtgctgttt cacgatgcga atcataccag gcttaatggc
     8881 ggtgagcagc ggtgcgtggc cagggtagat ccccagttca ccttcgctac ccgttacctg
     8941 gattttctcg accagaccag agaacatttg ttgctctgcg ctgacgacgt ccaggtggta
     9001 agtcattgcc atatcaccct ccgattaagg cgttaaagtt ttttggcttt ttccacagct
     9061 tcttcgatgg aaccgaccat gtagaacgcc tgctccggca ggtgatcgta ttcgccttcc
     9121 atgatgcctt taaagccacg gatggtgtct ttcagggaga cgtatttacc cggagaaccg
     9181 gtgaatacct ctgccacgaa gaacggctgg gacaggaagc gctggatctt acgagcacgc
     9241 gctaccacca gtttgtcttc ttcagacagt tcatccatac ccaggatggc gataatgtct
     9301 ttcagttcct gataacgttg caagatggac tgaacgccac gcgcggtgtc gtagtgttcc
     9361 tgaccaacca ccagcgggtc cagctgacgg ctggtggagt ccagcgggtc aacggccggg
     9421 tagataccca gagacgcgat ctgacggctc agtaccacgg ttgcgtcaag gtgcgcaaag
     9481 gtggttgccg gagatgggtc agtcaagtca tccgcaggta cgtataccgc ctgtacggag
     9541 gtgatagaac cagttttggt ggaggtgata cgttcctgca gaacacccat ctcttccgcc
     9601 agggtcggct gataacctac cgctgaaggc atacggccca gcagtgcgga tacttccgta
     9661 ccggccaggg tgtaacgata gatgttgtca acgaacagca gaacgtcacg accttcgtca
     9721 cggaatttct cagccatggt cagaccggtc agagcaacgc gcagacggtt tcccggcggc
     9781 tcgttcatct ggccatacac cagggatact ttgtcgataa cgttggagtc ggtcatttcg
     9841 tggtagaagt cgttaccctc acgagtacgt tcacctacgc ccgcaaacac agagtaaccg
     9901 gagtgctcga tcgcgatgtt acgaataagc tccatcatgt ttacggtttt acctacaccc
     9961 gcaccaccga acagaccaac tttaccgccc ttagcgaacg gacacatcag gtcgataact
    10021 ttgataccgg tttccagcaa ttcctgagag tttgacagct cttcgtagga aggtgctgcg
    10081 cggtgaatcg cccaacgctc ttcttcaccg atctcgcctt tcatgtcgac cggttcaccc
    10141 agtacgttca tgatacgacc cagagtcgct ttacctaccg ggacttcgat cgggtgttcg
    10201 aggtctttta catccagacc gcgacgcaga ccgtcggagg aacccattgc gatggtacgc
    10261 acgataccgc cgccgagctg ctgctgaact tccagcacca gacgctcatt accattttgc
    10321 acctcaagag catcgtacac gcgcggtacg gcatcctgag ggaattcgac gtcaactacg
    10381 gcgccgatta cctggacaat ctttccagta gccatcttaa atcctctacg aaataacctg
    10441 tttaaaccgc ggcggccccc gagacgatct cggtgagttc ctgagtaatg ctggcctgac
    10501 gagctttgtt gtataccaac tgcagctctt taatcaggct gccgccattg tcggtcgcgg
    10561 ctttcatcgc caccatacgg gcggcctgct cgctggccag gttttcaacc acgccctgat
    10621 aaacctgaga ttcgacataa cgacgcagca gggtatccag caacgccttc ggatcgggtt
    10681 cgtacaggta atcccaggat ttatgtttca gatcatcatc atctgatgcc ggtaacggca
    10741 gcagctggct gatggtcgga acctgagaca tggtgttaat aaatttgttg ctgacaatgt
    10801 aaagcttgtc cagacggcct tcgtcgtagg cctgcaacat cacttttacc ggaccgatca
    10861 gttcggacag ggaagggtta tcccccatgc cggtgacctg ggcaacaaca ttgccgccca
    10921 cggagttgaa gaacgacacg cctttcgagc cgatcattgc gaggtcgcat tgaacgcctt
    10981 tgtcggtcca ggtcttcatt tccgccagca gttttttgaa caggttaatg ttcaaaccac
    11041 cgcacaaacc acggtcggtc gacaccacca ggtagcccac gcgtttaacg tcgcggtctt
    11101 ccaggtaagg gtgcttatat tccagattac cgtgtgcaag gtgaccaatc actttgcgca
    11161 tggtttctgc ataaggacgg ctggccgcca tgcgatcctg cgatttacgc attttggaag
    11221 cggcgaccat ctccatcgct ttagtgatct tttgcgtgtt ctggacgctt gcgatcttac
    11281 tacgtatctc ttttgcgccg gccatgagct tctcctcaat gccttgcggc ctgccctaag
    11341 gcaagccgcc agacgttacc aggattgggt tgctttgaag gaatcgagga tgcctttcag
    11401 cttgccttcg atttcgtcgt tgtagccacc ggtctggttg atctcttgca tcaacggagc
    11461 gtgatcacgg tcgacgtaag ccagcagagc ggcttcgaag ctgccgattt tcgacagttc
    11521 aacatccgcc aggtaaccac gttctgctgc gaacagaacc agagactgtt gcgcaacaga
    11581 catcggcgca tactgtttct gcttcagcag ttcggtcact ttctgaccgt ggtcaagctg
    11641 cttacgtgtt gcatcgtcaa ggtcagatgc aaactgagag aacgctgcca gttcacgata
    11701 ctgtgccaga gcggtacgga taccaccgga cagttttttc atgatcttgg tctgtgctgc
    11761 accaccaaca cgggatacgg aaatacccgg gttaacagca ggacgaatac cggcgttgaa
    11821 caggttggtt tccaggaaga tctgaccatc ggtaatggag attacgttgg tcggaacgaa
    11881 cgcagaaacg tcacccgcct gagtttcgat aatcggcagc gcagtcagag aaccggtttt
    11941 ccctttcact tcacctttgg tgaaagcttc aacgtattcg gcgttaacac gcgcagcacg
    12001 ctccagcaga cgagagtgga ggtagaatac gtcgcccgga aatgcttcac gtcctggcgg
    12061 acgacggagc agcagggaaa tctgacggta agcaacagcc tgtttagaca ggtcatcgta
    12121 aatgatcagc gcatcttcac cgcggtcacg gaagtattca cccattgcgc aaccagcgta
    12181 cggtgccagg tattgcagtg ctgcggattc agacgcggtt gctaccacaa cgatggtgtt
    12241 agccagtgcg ccgtgctctt ccagtttacg taccacgtta gaaatggtgg acgctttctg
    12301 gccgatagcg acatagatac atttgatacc ggaatcgcgc tggttgatga tggcatcgat
    12361 agccagtgcg gttttaccgg tctgacggtc accgatgatc aattcacgct gaccacgacc
    12421 gattgggatc atggagtcaa cagctttata accggtctgt accggctgat ctacggactg
    12481 acgttcgata acgcccggag cgattgcttc tacagcagag aagccgtcgt gatccagcgg
    12541 acctttaccg tcgattggtg cacccagagt gttaaccaca cggcccagca ggccacggcc
    12601 aaccggaact tccaggatac ggccagtaca cttaactttc atgccttcgg caaggtcagc
    12661 gtacggaccc ataacaaccg cacctacaga gtcgcgctcg aggttcagtg cgatagcgta
    12721 acggttaccc ggcagggaga tcatttcacc ctgcatacaa tcggccaggc cgtgaatgcg
    12781 gataacaccg tcacttacag aaacaatagt accttcgttg tgagcttcac tcacaacatt
    12841 gaactgagca atgcgctgct tgatcagttc gctgatttcg gtggaattca gttgcatgct
    12901 ccagtcccct taagactgca agacgtctgc aaggcgctca agacgaccgc gtacgctgcc
    12961 atcaatgacc atatcacccg ctcggatgat aacgcctgcc attacagact tatcgatttt
    13021 gcaattcagc ttaactttgc gtgacagacg tttttccatc gcagcagaaa ttttcgcgag
    13081 ctgttgttca ctcagtgcgg cagcggaaat gacgtctacc tcagtggtag cctcactcac
    13141 ggcacgcagg tgaataaact gctccagaac atccgggagc gccttaagac gaccattttc
    13201 agccattacc cgaatcaggt tctgaccgtt ttcgtccagt tgctcaccac aaactgcgat
    13261 aaacgactcg gcgagcgttt ctggcgcaag cgcgccagag agaagctctg ccatttgttc
    13321 gtttttggtt acctcggcgg caaacgccag catgtcctgc cagcgttcta cactttggtg
    13381 ttcgacggca aagtcaaaag ctgctttggc gtaggggcga gctaccgtaa taaattcaga
    13441 catcagcccc tccctcctta cagttcagcg acaagtttat ccacgatgtc gctgttagca
    13501 gcttcatcca cggaacgttc gatgatcttc tcggcgccag caacagccag gatagcaact
    13561 tgcttacgca gctcttcacg ggcacgttta cgctcggctt caatttccgc ctgcgcctgg
    13621 gccacgattt tagtacgttc ctgttctgcc tcagctttcg cttcgtccag aatctgcgag
    13681 cggcgtttgt tcgcctgctc gatgattacc tgggcttccg ctttcgcttt tttcagctgg
    13741 tcggtcgcgc tggcctttgc aaggtcaagg tccttatgtg ctcgttctgc ggaagcaagg
    13801 ccgtcagcaa tttctttttg acgtttttcg atggctgcca ttaatggcgg ccatacgtac
    13861 ttcatgcaga acagaacgaa caggacaaac gcgatggcct ggccgaggat tgttgcgtta
    13921 agattcacag cacaatgcct ctatttagtt aacgttctga tattgctctt taaataaaag
    13981 caacgcttac tacgcgacag cgaacatcac gtacagaccc agacctacag cgatcatcgg
    14041 gatagcatcc accagaccca taacgataaa gaactgagta cgcagcagag gaatcagatc
    14101 aggttgacgc gctgcgcctt ccaggaattt acccccgagg atgccgatac cgatcgcagc
    14161 accgattgcc gccagaccca tcatcacagc ggcagccatg tacagcagat ccatattcag
    14221 gttttccatg acagtctcca gtttgtttca gttaaaacgt agtagtgttg gtaaattaat
    14281 gttcttcaga cgccatcgac agatagacga tcgtcagaac catgaagatg aaggcttgca
    14341 gcgtaatgat caggatgtgg aaaatggccc acggcacatt cagaatccac tgtgaccacc
    14401 acggcaacag accagcaatc agaatgaaaa tcagctcacc ggcatacatg ttaccgaaca
    14461 gtcgcaaacc gagtgaaact ggtttggaca gcaggcttac cccttcaagg attaagttga
    14521 caggaatgaa cgcccagtga ttgaacggct gcagcgtcaa ctctttcgtg aagccgccga
    14581 tgcctttcat tttgatgctg tagaacagaa tcaggataaa tacgcccagt gccatagaca
    14641 gcgttacgtt cacgtcagca gacggaacca cacgcagtgc aggcagaccc agtacatgtt
    14701 cagcaatgta cggcagcagg tcgataggca gtaaatccat caggttcatc aggaataccc
    14761 agacgaagat cgtcagggcc agcggagcaa tcagcttgct tttgccatgg tacatgtctt
    14821 tcacgctacc attaacaaat ccgatcacca gctcaatcgc ggtctgaaac ttacctggca
    14881 caccgctggt cgcctttttg gctacgctac ggaataaaac caggaacaac agacccagca
    14941 ccaccgagaa gaacatggag tcaatattga ttgtccagaa ggtggctggg gggttatgtg
    15001 gatccaccag cgagaatgta cgcaggtcca gctgaaggtt attcaggtgg tgtcctatgt
    15061 aatcctgcgg cgtcatattt tctgaagcca tgatgccttt taccctttgt tgttaattac
    15121 agccggtgcc agtatctgaa ccaccagcac caaaacccac gtaacgatca gcggcaagaa
    15181 taccgccttt aaaaccgcca acgccaccac cagtaacacc aacatcgcca gaactttgaa
    15241 agcttcgcca aatgcgaatg tccaggccac ccggcctttc gctggtgtat gcgcctggtg
    15301 acgccaggca aatatcataa ataaaacgtt aggcagaaag actgccaggc ccccgcttat
    15361 tgcagagacg ccccagaagg ggtctttgag gctgaacagc aatccacttg ctatcaccac
    15421 cagtaactga acgagcagaa gcttccgagc aacgtttcga ctcacgagcg acacagacat
    15481 cacgtttttc actcctgctc ccttcgaggt atgccgcgtg tcgtataaaa ctttctttaa
    15541 ggcttagagt caagcatcaa aaagcggtca aattatacgg tgcgcccccg tgatttcaaa
    15601 caataagtag ccaaaaggtg aataaatgtt taaatatttt tccagtgcat acaattgcga
    15661 cttttctgca aaccctgttc gatcatgaaa aactgtaaat aacgcgtaaa cactggtgat
    15721 aaagcgtgct tcagatcaca tattgcgcat gttcgcgcac agcatattta tttacttggc
    15781 aaatgatgcc tttgcaagtt tatgatattt cagtctaaaa acagatactg ttttaataaa
    15841 tgacatttac ataacaaaaa ccagccattg a
//
LOCUS       DAHHTG010000100        15576 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.101, whole genome shotgun sequence.
ACCESSION   DAHHTG010000100 DAHHTG010000000
VERSION     DAHHTG010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15576)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15576)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15576
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.101"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            266..670
                     /gene="sdhC"
                     /locus_tag="NBI74_003288"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
     CDS             266..670
                     /gene="sdhC"
                     /locus_tag="NBI74_003288"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000263334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HCD1933572.1"
                     /translation="MWALFMIRNVKKQRPVNLDLQTIRFPVTAIASILHRVSGVITFV
                     AVGILLWLLGTSLSSPEGFEQASAIMGSFFVKFIMWGILTALAYHVVVGIRHMMMDFG
                     YLEETFEAGKRSAKISFVITVVLSLLAGVLVW"
     gene            664..1011
                     /gene="sdhD"
                     /locus_tag="NBI74_003289"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
     CDS             664..1011
                     /gene="sdhD"
                     /locus_tag="NBI74_003289"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase membrane anchor subunit"
                     /protein_id="HCD1933573.1"
                     /translation="MVSNASALGRNGVHDFILVRATAIVLTLYIIYMVGFFATSGELT
                     YEVWIGFFASAFTKVFTLLALFSILIHAWIGMWQVLTDYVKPLALRLMLQLVIVVALV
                     VYVIYGFVVVWGV"
     gene            1011..2777
                     /gene="sdhA"
                     /locus_tag="NBI74_003290"
     CDS             1011..2777
                     /gene="sdhA"
                     /locus_tag="NBI74_003290"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HCD1933574.1"
                     /translation="MKLPVREFDAVVIGAGGAGMRAALQISQSGQTCALLSKVFPTRS
                     HTVSAQGGITVALGNTHEDNWEWHMYDTVKGSDYIGDQDAIEYMCKTGPEAILELEHM
                     GLPFSRLDDGRIYQRPFGGQSKNFGGEQAARTAAAADRTGHALLHTLYQQNLKNHTTI
                     FSEWYALDLVKNQDGAVVGCTALCIETGEVVYFKARATVLATGGAGRIYQSTTNAHIN
                     TGDGVGMAIRAGVPVQDMEMWQFHPTGIAGAGVLVTEGCRGEGGYLLNKHGERFMERY
                     APNAKDLAGRDVVARSIMIEIREGRGCDGPWGPHAKLKLDHLGKEVLESRLPGILELS
                     RTFAHVDPVKEPIPVIPTCHYMMGGIPTKVTGQALTVNEKGEDVVVPGLFAVGEIACV
                     SVHGANRLGGNSLLDLVVFGRAAGLHLQESIAEQGALRDASESDVEASLDRLNRWNNN
                     RNGEDPVAIRKALQECMQHNFSVFREGDAMAKGLEQLKVIRERLKNARLDDTSSEFNT
                     QRVECLELDNLMETAYATAVSANFRTESRGAHSRFDFPDRDDENWLCHSLYLPESESM
                     TRRSVNMEPKLRPAFPPKIRTY"
     gene            2793..3509
                     /gene="sdhB"
                     /locus_tag="NBI74_003291"
     CDS             2793..3509
                     /gene="sdhB"
                     /locus_tag="NBI74_003291"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit
                     SdhB"
                     /protein_id="HCD1933575.1"
                     /translation="MRLEFSIYRYNPDVDDAPRMQDYTLEAEEGRDMMLLDALIQLKE
                     KDPSLSFRRSCREGVCGSDGLNMNGKNGLACITPISALNQPGKKIVIRPLPGLPVIRD
                     LVVDMGQFYAQYEKIKPYLLNNGQNPPAREHLQMPEQREKLDGLYECILCACCSTSCP
                     SFWWNPDKFIGPAGLLAAYRFLIDSRDTETDSRLDGLSDAFSVFRCHSIMNCVSVCPK
                     GLNPTRAIGHIKSMLLQRNA"
     gene            3871..6672
                     /gene="sucA"
                     /locus_tag="NBI74_003292"
     CDS             3871..6672
                     /gene="sucA"
                     /locus_tag="NBI74_003292"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HCD1933576.1"
                     /translation="MQNSALKAWLDSSYLSGANQSWIEQLYEDFLTDPDSVDANWRST
                     FQQLPGTGVKPDQFHSQTREYFRRLAKDASRYSSTISDPDTNVKQVKVLQLINAYRFR
                     GHQHANLDPLGLWQQDKVADLDPSFHDLTEADFQETFNVGSFASGKETMKLGELLEAL
                     KQTYCGPIGAEYMHITSTEEKRWIQQRIESGRATFNSEEKKRFLSELTAAEGLERYLG
                     AKFPGAKRFSLEGGDALIPMLKEMIRHAGNSGTREVVLGMAHRGRLNVLVNVLGKKPQ
                     DLFDEFAGKHKEHLGTGDVKYHMGFSSDFQTDGGLVHLALAFNPSHLEIVSPVVIGSV
                     RARLDRLDEPSSNKVLPITIHGDAAVTGQGVVQETLNMSKARGYEVGGTVRIVINNQV
                     GFTTSNPLDARSTPYCTDIGKMVQAPIFHVNADDPEAVAFVTRLALDFRNTFKRDVFI
                     DLVCYRRHGHNEADEPSATQPLMYQKIKKHPTPRKIYADKLEQEKVATLEDATEMVNL
                     YRDALDAGDCVVAEWRPMNMHSFTWSPYLNHEWDEEYPNKVEMKRLQELAKRISTVPE
                     AVEMQSRVAKIYGDRQAMAAGEKLFDWGGAENLAYATLVDEGIPVRLSGEDSGRGTFF
                     HRHAVIHNQSNGSTYTPLQHIHNGQGAFRVWDSVLSEEAVLAFEYGYATAEPRTLTIW
                     EAQFGDFANGAQVVIDQFISSGEQKWGRMCGLVMLLPHGYEGQGPEHSSARLERYLQL
                     CAEQNMQVCVPSTPAQVYHMLRRQALRGMRRPLVVMSPKSLLRHPLAVSSLEELANGT
                     FLPAISEIDELDPKGVKRVVMCSGKVYYDLLEQRRKNNQHDVAIVRIEQLYPFPHKAM
                     QEVLQQFAHVKDFVWCQEEPLNQGAWYCSQHHFREVIPFGASLRYAGRPASASPAVGY
                     MSVHQKQQQDLVNDALNVE"
     gene            6687..7904
                     /gene="odhB"
                     /locus_tag="NBI74_003293"
     CDS             6687..7904
                     /gene="odhB"
                     /locus_tag="NBI74_003293"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HCD1933577.1"
                     /translation="MSSVDILVPDLPESVADATVATWHKKPGDVVVRDEVLVEIETDK
                     VVLEVPASADGILDAVLEDEGTTVTSRQILGRLREGNSAGKETSAKSEEKASTPAQRQ
                     QASLEEQNNDALSPAIRRLLAEHNLDASAIKGTGVGGRLTREDVEKHLAKAPAKESAP
                     AAAAPAAQPALAARSEKRVPMTRLRKRVAERLLEAKNSTAMLTTFNEVNMKPIMDLRK
                     QYGEAFEKRHGIRLGFMSFYVKAVVEALKRYPEVNASIDGDDVVYHNYFDVSMAVSTP
                     RGLVTPVLRDVDTLGMADIEKKIKELAVKGRDGKLTVEDLTGGNFTITNGGVFGSLMS
                     TPIINPPQSAILGMHAIKDRPMAVNGQVEILPMMYLALSYDHRLIDGRESVGFLVTIK
                     ELLEDPTRLLLDV"
     gene            7998..9164
                     /gene="sucC"
                     /locus_tag="NBI74_003294"
     CDS             7998..9164
                     /gene="sucC"
                     /locus_tag="NBI74_003294"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HCD1933578.1"
                     /translation="MNLHEYQAKQLFARYGLPAPVGYACTTPREAEEAASKIGAGPWV
                     VKCQVHAGGRGKAGGVKVVNSKEDIRAFAENWLGKRLVTYQTDANGQPVNQILVEAAT
                     DIAKELYLGAVVDRSSRRVVFMASTEGGVEIEKVAEETPHLIHKVALDPLTGPMPYQG
                     RELAFKLGLEGKLVQQFTKIFMGLATIFLERDLALIEINPLVITKQGDLICLDGKLGA
                     DGNALFRQPDLREMRDQSQEDPREAQAAQWELNYVALDGNIGCMVNGAGLAMGTMDIV
                     KLHGGEPANFLDVGGGATKERVTEAFKIILSDDKVKAVLVNIFGGIVRCDLIADGIIG
                     AVAEVGVNVPVVVRLEGNNAELGAKKLADSGLNIIAAKGLTDAAQQVVAAVEGK"
     gene            9164..10033
                     /gene="sucD"
                     /locus_tag="NBI74_003295"
     CDS             9164..10033
                     /gene="sucD"
                     /locus_tag="NBI74_003295"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HCD1933579.1"
                     /translation="MSILIDKNTKVICQGFTGSQGTFHSEQAIAYGTKMVGGVTPGKG
                     GTTHLGLPVFNTVREAVAATGATASVIYVPAPFCKDSILEAIDAGIKLIITITEGIPT
                     LDMLTVKVKLDEAGVRMIGPNCPGVITPGECKIGIQPGHIHKPGKVGIVSRSGTLTYE
                     AVKQTTDYGFGQSTCVGIGGDPIPGSNFIDILEMFEKDPQTEAIVMIGEIGGSAEEEA
                     AAYIKEHVTKPVVGYIAGVTAPKGKRMGHAGAIIAGGKGTADEKFAALEAAGVKTVRS
                     LADIGEALKTVLK"
     gene            complement(10137..10859)
                     /gene="mngR"
                     /locus_tag="NBI74_003296"
     CDS             complement(10137..10859)
                     /gene="mngR"
                     /locus_tag="NBI74_003296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-D-glycerate transport/metabolism system
                     transcriptional repressor MngR"
                     /protein_id="HCD1933580.1"
                     /translation="MGHKPLYRQIADRIREQITCGELKPGDALPTESALQTEFGVSRV
                     TVRQALRQLVEQQILESIQGSGTYVKEERVNYDIFQLTSFDEKLSDRHVETHSEVLIF
                     EVIPADNFLQQQLQISEQDRVWHVKRVRYRKQKPVALEETWMPLALFPDLTWQVMENS
                     KYHFIEEVKKMVIDRSEQEIIPLMPTEEMSRLLNISQTKPILEKVSRGYLVDGQVFEY
                     SRNAFNTDDYKFTLIAQRKSPR"
     gene            11028..12944
                     /gene="mngA"
                     /locus_tag="NBI74_003297"
     CDS             11028..12944
                     /gene="mngA"
                     /locus_tag="NBI74_003297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001393949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS 2-O-a-mannosyl-D-glycerate transporter
                     subunit IIABC"
                     /protein_id="HCD1933581.1"
                     /translation="MNLTTLTHRDALCLNVRFTSREEAIHALTQRLAALGKISSTEQF
                     LEEVYCRESLGPTALGEGLAVPHGKTAAVKEAAFAVATLSEPLQWEGVDGPEAVDLVV
                     LLAIPPNEAGTTHMKLLTALTTRLADDELRARIQSATTPDELLSALDDKGDAQHTASF
                     SYAPTIVCVTACPAGIAHTYMAAEYLEKAGRKLGVNIYVEKQGANGIEERLTAEQLNS
                     ATACIFAAEVAIKESERFNGIPALSVPVAEPIRHAEALIQQALTLKRSDETRTVQQDT
                     QPVKSVKTELKQALLSGISFAVPLIVAGGTVLAVAVLLSQIFGLQDLFNEENSWLWMY
                     RKLGGGMLGILMVPVLAAYTAYSLADKPALAPGFAAGLAANMIGSGFLGAVVGGLIAG
                     YLMRWVKNHLRLSSKFNGFLTFYLYPVLGTLGAGSLMLFVVGEPVAWINNSLTAWLNG
                     LSGNNALLLGAILGFMCSFDLGGPVNKAAYAFCLGAMANGVYGPYAIFASVKMVSAFT
                     VTASTMLAPRLFKEFEIETGKSTWLLGLAGITEGAIPMAIEDPLRVIGSFVLGSMVTG
                     AIVGAMNIGLSTPGAGIFSLFLLHDNGAGGVMAAIGWFGAALVGAAISTAILLIWRHH
                     AVKHGNYLTDGVMP"
     gene            12962..>15576
                     /gene="mngB"
                     /locus_tag="NBI74_003298"
     CDS             12962..>15576
                     /gene="mngB"
                     /locus_tag="NBI74_003298"
                     /EC_number="3.2.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosylglycerate hydrolase"
                     /protein_id="HCD1933582.1"
                     /translation="MKAVSRVHITPHMHWDREWYFTTEESRILLVNNMEEILCRLEQD
                     NEYKYYVLDGQTAILEDYFAVKPENKDRVKKLVEGGKLIIGPWYTQTDTTIVSAESIV
                     RNLMYGMRDCLAFGEPMKIGYLPDSFGMSGQLPHIYNGFGITRTMFWRGCSERHGTDK
                     TEFLWQSSDGSEVTAQVLPLGYAIGKYLPADENGLRKRLDSYFDVLEKASVTKEILLP
                     NGHDQMPLQQNIFEVMEKLREIYPQRKFVMSRFEEVFEQIEAQRESLAILKGEFIDGK
                     YMRVHRTIGSTRMDIKIAHARIENKIVNLLEPLATLAWTLGFDYHHGLLEKMWKEILK
                     NHAHDSIGCCCSDKVHREIVARFELAEDMADNLIRFYMRKIADNMPQSDADKLVLFNL
                     MPWPREEVINTTVRLRGSQFNLRDGRGQPVPYFIRHAREIDPGLIDRQIVHYGNYDPF
                     MEFDIQISQIVPSMGYRTLYIEANQPGNVVTPKSKTEGILENAFWQIALNEDGSLRLV
                     DKDSGVRYDRVFQIEEGSDDGDEYDYSPAKEEWAITSANAKPQYDIIHEAWQSRAIIR
                     YEIAVPRNLSERRAKQCSGRVGVETVITLSHNSRRIDADINLDNQADDHRIRVLIPTP
                     FNTDVVLADTQFGSLTRPVKDCAMNVWQQEGWKEAPVPVWNMLNYAVLQEGRNGIAVF
                     SEGLREFEVIGEENKTFAITLLRGVGLLGKEDLLLRPGRPSGIKMPVSDSQLRGSFSC
                     RLSLFSYIGTPVTAGVAQQARAWLTPVQCYNKIPWDAMKLNKAKFNVPESYSLLKMPP
                     VGCLISALKKAEDRQELILRLFNPAELTSCDATVAFSRKVMTCTETMMNERFNTEKNE
                     VLKELALFLPGQSRT"
BASE COUNT         3760 a         3859 c         4288 g         3669 t
ORIGIN      
        1 ccgacaaact atatgtaggt taattgtaat gattttgtga acagcctata ctgccgccag
       61 gtctccggaa caccctgcaa tcccgagcca cccagcgttg taacgtgtcg ttttcgcatc
      121 tggaagcagt gttttgcatg acgcgcagtt atagaaagga cgctgtctga cccgcaagca
      181 gaccggagga aggaaatccc gacgtctcct ggtaacagaa agttaacctc tgtgcccgta
      241 gtccccaggg aataataaga acagcatgtg ggcgttattc atgataagaa atgtgaaaaa
      301 acaaagacct gttaatctgg acctacagac catccggttc cccgtcacgg cgatagcgtc
      361 cattctccat cgcgtttccg gtgtgatcac ctttgttgca gtgggcatcc tgctgtggct
      421 tctgggtacc agcctctctt cccctgaagg tttcgagcaa gcttccgcga ttatgggcag
      481 cttcttcgtc aaatttatca tgtggggcat ccttaccgct ctggcatatc acgtcgtcgt
      541 aggtattcgc cacatgatga tggattttgg ctatctggaa gaaacattcg aagcgggtaa
      601 acgctccgcc aaaatctcct ttgttattac tgtcgtgctt tcacttctcg caggagtcct
      661 cgtatggtaa gcaacgcctc cgcattagga cgcaatggcg tacatgattt catcctcgtt
      721 cgtgctaccg ctatcgtcct gacgctctac atcatttata tggtcggttt tttcgctacc
      781 agtggcgagc tgacatatga agtctggatt ggtttcttcg cctctgcgtt caccaaagtg
      841 ttcaccctgc tggcgctgtt ttctatcttg atccatgcct ggatcggcat gtggcaggtg
      901 ttgaccgact acgttaaacc gctggccttg cgcctgatgc tgcaactggt gattgtcgtt
      961 gcactggtgg tttacgtgat ttatggattc gttgtggtgt ggggtgtgtg atgaaattgc
     1021 cagtcagaga atttgatgca gttgtgattg gtgctggcgg cgcaggtatg cgcgcggcgc
     1081 tgcaaatttc ccagagtggc cagacctgtg cgctgctctc taaggtcttc ccgacccgtt
     1141 cccataccgt ttctgcgcaa ggtggtatta ccgttgcgct gggtaatacc catgaagata
     1201 actgggaatg gcatatgtac gacaccgtaa aagggtcgga ctatatcggt gaccaggacg
     1261 cgattgaata tatgtgtaaa accgggccgg aagcgattct ggaactggaa catatgggcc
     1321 tgccgttctc gcgtcttgat gatggtcgta tctatcaacg tccgtttggc ggtcagtcga
     1381 aaaacttcgg cggcgagcag gcggcacgta ctgcggcggc agctgaccgt accggtcacg
     1441 cactgttgca cacgctttat cagcagaacc tgaaaaacca caccaccatt ttctccgagt
     1501 ggtatgcgct ggatctggtg aaaaaccagg atggcgcagt ggtcggttgt accgcactgt
     1561 gcatcgaaac tggtgaagtg gtttacttta aagctcgcgc gacagtgctg gcgactggcg
     1621 gggcagggcg tatttatcag tccaccacca acgcccacat taacactggc gacggtgtcg
     1681 gcatggctat ccgtgcaggc gtaccggtgc aggatatgga aatgtggcag ttccacccga
     1741 ccggtattgc cggtgcgggc gtactggtca ccgaagggtg ccgtggtgaa ggcggttatc
     1801 tgctgaacaa acatggcgaa cgctttatgg agcgttatgc gccgaacgcc aaagacctgg
     1861 cgggccgtga cgtggtggcg cgttccatca tgatcgaaat ccgtgaaggt cgcggctgtg
     1921 atggtccgtg ggggccacac gcaaaactga aacttgacca tctggggaaa gaagttctcg
     1981 aatcccgtct gccgggtatt ctggaactct cccgtacctt cgctcacgtc gatccggtga
     2041 aagagccgat tccggttatc ccaacctgtc actacatgat gggcggtatt ccgaccaaag
     2101 tgaccggtca ggcgctgacg gtgaatgaga aaggcgaaga tgtggttgtt ccggggctgt
     2161 ttgccgttgg tgaaatcgct tgtgtatcgg tacacggcgc taaccgtctg ggcggcaact
     2221 cgctgctgga cctggtggta tttggtcgtg cggcaggtct gcatctgcaa gagtctatcg
     2281 ccgagcaggg cgcactgcgc gatgccagcg agtctgatgt agaagcgtct ctggatcgcc
     2341 tgaaccgctg gaacaataac cgtaacggtg aagatccggt ggcgatccgt aaagcactgc
     2401 aagaatgtat gcagcataac ttctcggtct tccgtgaagg tgatgcgatg gcgaaagggc
     2461 ttgagcagtt gaaagttatc cgcgagcgtc tgaaaaatgc ccgtctggat gacacttcca
     2521 gcgagttcaa cacccagcgc gttgagtgcc tggaactgga taacctgatg gaaacggcgt
     2581 atgcaacggc tgtttctgcc aacttccgta ccgaaagccg tggcgcgcat agccgcttcg
     2641 acttcccgga tcgtgatgat gaaaactggc tgtgccactc cctgtatctg ccagagtcgg
     2701 aatccatgac gcgccgaagc gtcaacatgg aaccgaaact gcgcccggca ttcccgccga
     2761 agattcgtac ttactaatgc ggagacagga aaatgagact cgagttttca atttatcgct
     2821 ataacccgga tgttgatgat gctccgcgta tgcaggatta caccctggaa gcggaagaag
     2881 gtcgcgacat gatgctgctg gatgcgctta ttcagctgaa agagaaagat cccagcctgt
     2941 cgttccgccg ctcctgccgt gaaggtgtgt gcggttccga cggtctgaac atgaacggta
     3001 agaatggtct ggcctgtatt accccgattt cggcactcaa ccagccgggc aagaagattg
     3061 tgattcgtcc gctgccaggt ttaccggtga tccgcgattt ggtggtagac atgggacaat
     3121 tctatgcgca atatgagaaa attaagcctt acctgttgaa taatggacaa aatccgccag
     3181 ctcgcgagca tttacagatg ccagagcagc gcgaaaaact cgacgggttg tatgaatgta
     3241 ttctctgcgc atgttgttca acctcttgtc cgtctttctg gtggaatccc gataagttta
     3301 tcggtccggc aggcttgtta gcggcatatc gtttcctgat cgatagccgt gataccgaga
     3361 ctgacagccg cctcgacggt ttgagcgatg cattcagtgt attccgctgt cacagcatca
     3421 tgaactgcgt cagtgtatgt ccgaaggggc tgaacccgac gcgcgccatc ggccatatca
     3481 agtcgatgtt gttgcaacgt aatgcgtaaa ccgtaggcct gataagacgc acaagcgtcg
     3541 catcaggcaa ccagtgccgg atgcggcgtg aacgccttat ccggcctaca aaagaaatgc
     3601 agaaaccgta ggcctgataa gacgcgcaag cgtcgcatca ggcaaacagc gccggatgcg
     3661 gcgtgaatgc cttatccggc ctacaaaaga aatgcaggaa atctttaaaa actgcccctg
     3721 acactaagac agtttttaaa ggttccttag cggacgcaga aaacacgaca agtccgcaac
     3781 aagtgaaccc cggcacgcac atcagatatg aaatgtgcgt ggtagtatcc acggcgaagt
     3841 aagcataaaa aagatgctta agggatcacg atgcagaaca gcgctttgaa agcctggttg
     3901 gactcttctt acctctctgg cgcaaaccag agctggatag aacagctcta tgaagacttc
     3961 ttaaccgatc ctgactcggt tgacgctaac tggcgttcga cgttccagca gttacctggt
     4021 acgggagtca aaccggatca attccactct caaacgcgtg aatatttccg ccgcctggcg
     4081 aaagacgctt cacgttactc ttcaacgatc tccgaccctg acaccaatgt gaagcaggtt
     4141 aaagtcctgc agctcattaa cgcataccgc ttccgtggtc accagcatgc gaatctcgat
     4201 ccgctgggac tgtggcagca agataaagtg gccgatctgg atccgtcttt ccacgatctg
     4261 accgaagcag acttccagga gaccttcaac gtcggttcat ttgccagcgg caaagaaacc
     4321 atgaaactcg gcgagctgct ggaagccctc aagcaaacct actgtggccc gattggtgcc
     4381 gagtatatgc acattaccag caccgaagaa aaacgctgga tccaacagcg tatcgagtct
     4441 ggtcgcgcga ctttcaatag cgaagagaaa aaacgcttct taagcgaact gaccgccgct
     4501 gaaggccttg aacgttacct tggcgcaaaa ttccctggcg caaaacgctt ctcgctggaa
     4561 ggcggtgacg cgttaatccc gatgcttaaa gagatgatcc gccacgctgg caacagcggc
     4621 acccgcgaag tggttctcgg gatggcgcac cgtggtcgtc tgaacgtgct ggtgaacgtg
     4681 ctgggtaaaa aaccgcaaga cttgttcgac gagtttgccg gtaaacataa agaacacctc
     4741 ggcacgggcg acgtgaaata ccacatgggc ttctcgtctg acttccagac cgatggcggc
     4801 ctggtgcacc tggcgctggc gtttaacccg tctcaccttg agattgtaag cccggtcgtt
     4861 atcggttctg ttcgtgcccg tctggacaga cttgatgagc cgagcagcaa caaagtgctg
     4921 ccaatcacca ttcatggtga cgccgcagtg accgggcagg gcgtagttca ggaaaccctg
     4981 aacatgtcga aagcgcgtgg ttatgaagtt ggcggtacgg tacgtatcgt tatcaacaac
     5041 caggttggct tcaccacctc taacccgctg gatgcccgtt cgacgccgta ctgtactgat
     5101 atcggtaaga tggttcaggc accgattttc cacgttaatg cggatgatcc ggaagccgtt
     5161 gcctttgtga cccgtctggc gctcgatttc cgtaacacct ttaaacgtga tgtcttcatc
     5221 gacctggtat gctaccgccg tcacggccac aacgaagccg acgagccgag cgcaacccag
     5281 ccgctgatgt atcagaaaat caaaaaacat ccgacgccgc gcaaaatcta cgctgacaag
     5341 ctggagcagg aaaaagtcgc gacgctggaa gatgccaccg agatggttaa cctgtaccgc
     5401 gatgcgctgg atgctggcga ttgcgttgta gcagagtggc gtccgatgaa catgcactct
     5461 ttcacctggt cgccgtacct caaccacgaa tgggacgaag agtacccgaa caaagttgag
     5521 atgaagcgcc tgcaggaact ggctaaacgc atcagcacgg tgccggaagc ggttgaaatg
     5581 cagtctcgcg ttgccaaaat ttatggcgat cgccaggcga tggcagccgg tgagaaactg
     5641 ttcgactggg gcggcgcgga aaacctcgct tacgccacgt tggttgacga aggcattccg
     5701 gttcgcctgt cgggtgaaga ctccggtcgc ggtaccttct tccaccgcca cgcggtgatc
     5761 cacaaccagt ctaacggttc cacttacacg ccgctgcaac atatccataa cggccagggc
     5821 gcgttccgcg tctgggactc tgtactgtct gaagaagccg tactggcgtt tgaatacggt
     5881 tatgccaccg cagaaccacg caccctgacc atctgggaag cacagttcgg tgacttcgcc
     5941 aacggtgcac aggtggttat cgaccagttc atctcctctg gcgaacagaa atggggccgg
     6001 atgtgtggcc tggtgatgtt gctgccgcac ggttacgaag ggcaggggcc ggagcactcc
     6061 tccgcgcgtc tggaacgtta tctgcaactt tgcgctgagc aaaacatgca ggtttgcgta
     6121 ccttctaccc cggcacaggt ttaccacatg ctgcgtcgtc aggcgttgcg cgggatgcgt
     6181 cgtccactgg tcgtgatgtc gccgaaatcc ctgctgcgtc atccgctggc ggtttccagc
     6241 ctcgaagaac tggcgaacgg caccttcctg ccagccatca gtgaaatcga cgagcttgat
     6301 ccgaagggcg tgaagcgcgt agtgatgtgt tctggtaagg tttattacga tctgctggaa
     6361 caacgtcgta agaacaatca acacgatgtc gccattgtgc gtatcgagca actctacccg
     6421 ttcccgcata aagcgatgca ggaagtgttg cagcagtttg ctcacgtcaa ggattttgtc
     6481 tggtgccagg aagagccgct caaccagggc gcatggtact gcagccagca tcatttccgt
     6541 gaagtgattc cgtttggggc ttctctgcgt tatgcaggcc gcccggcctc cgcctctccg
     6601 gcggtagggt atatgtccgt tcaccagaaa cagcaacaag atctggttaa tgacgcgctg
     6661 aacgtcgaat aaataaagga tacacaatga gtagcgtaga tattctggtc cctgacctgc
     6721 ctgaatccgt agccgatgcc accgtcgcaa cctggcataa aaaacccggc gacgtagtcg
     6781 tacgtgatga agtgctggta gaaatcgaaa ctgacaaagt ggtactggaa gtaccggcat
     6841 cagcagacgg cattctggat gcggttctgg aagatgaagg tacaaccgta acgtctcgtc
     6901 agatccttgg tcgcctgcgt gaaggcaaca gcgccggtaa agaaaccagc gccaaatctg
     6961 aagagaaagc gtccactccg gcgcaacgcc agcaggcgtc tctggaagag caaaacaacg
     7021 atgcgttaag cccggcgatc cgtcgcctgc tggctgaaca taacctcgac gccagcgcca
     7081 ttaaaggcac gggtgtgggt ggtcgtctga cccgtgaaga tgtggaaaaa catctggcga
     7141 aagccccggc gaaagagtct gctccggcag cggctgctcc ggcggcgcaa ccggccctgg
     7201 ctgcacgtag cgaaaaacgt gtgccgatga ctcgcctgcg taagcgtgtg gcagagcgtc
     7261 tgctggaagc gaaaaactcc accgccatgc tgaccacgtt caacgaagtc aacatgaagc
     7321 cgattatgga tctgcgtaag cagtacggtg aagcgtttga aaaacgccac ggcatccgtc
     7381 tgggctttat gtccttctac gtgaaagcgg tggttgaagc cctgaaacgt tacccggaag
     7441 tgaatgcgtc tatcgacggc gatgatgtgg tctaccacaa ctatttcgac gtcagtatgg
     7501 cggtttctac gccgcgcggc ctggtgacgc cggttctgcg tgatgtcgat accctcggca
     7561 tggcagacat cgagaagaaa attaaagagc tggcagttaa aggccgtgac ggcaagctga
     7621 cggttgaaga tctgactggt ggtaacttta ccatcaccaa cggtggtgtg ttcggttccc
     7681 tgatgtctac gcctatcatc aacccgccgc agagcgcaat tctgggtatg cacgctatca
     7741 aagatcgtcc gatggcggtg aatggccagg ttgagatcct gccgatgatg tacctggcgc
     7801 tgtcctacga tcaccgtttg atcgatggtc gcgaatccgt gggcttcctg gtaacgatca
     7861 aagagttgct ggaagatccg acgcgtctgc tgctggacgt gtagtagttt aagcttcacc
     7921 tgcactgtag accgacgtgg atagggggtc ggtctacggt ttaaaagata acgattactg
     7981 aaggatggac agaacacatg aacttacatg aatatcaggc aaaacaactt tttgcccgct
     8041 atggcttacc agcaccggtg ggttatgcct gtactactcc acgcgaagca gaagaagccg
     8101 cttcaaaaat cggtgccggt ccgtgggtag tgaaatgtca ggttcacgct ggtggccgcg
     8161 gtaaagcggg cggtgtgaaa gttgtaaaca gcaaagaaga catccgtgct tttgcagaaa
     8221 actggctggg caagcgtctg gtaacgtatc aaacagatgc caatggccaa ccggttaacc
     8281 agattctggt tgaagcagcg accgatatcg ctaaagagct gtatctcggt gccgttgttg
     8341 accgtagttc ccgtcgcgtg gtctttatgg cctccaccga aggcggcgtg gaaatcgaaa
     8401 aagtggcgga agaaactccg cacctgatcc acaaagttgc gcttgatccg ctgactggcc
     8461 cgatgccgta tcagggacgc gagctggcgt ttaaactggg tctggaaggt aaactggttc
     8521 agcagttcac caaaatcttc atgggcctgg cgaccatttt cctggagcgc gaccttgcgc
     8581 ttatcgaaat caacccgctg gttattacca aacagggcga tctgatttgc ctcgacggca
     8641 aactgggcgc tgatggcaac gcactgttcc gccagcctga tctgcgtgaa atgcgtgacc
     8701 agtcgcagga agatccgcgt gaagcacagg ctgcacagtg ggaactgaac tacgttgcgc
     8761 tggacggtaa catcggttgt atggttaacg gcgcaggtct ggcgatgggt acgatggaca
     8821 tcgttaaact gcacggcggc gaaccggcta acttcctgga cgttggcggt ggcgcaacca
     8881 aagaacgtgt aaccgaagcg ttcaaaatca tcctctctga cgacaaagtg aaagccgttc
     8941 tggttaacat cttcggcggt atcgttcgtt gcgacctgat cgctgacggt atcatcggcg
     9001 cggtagcaga agtgggtgtt aacgtaccgg tcgtggtacg tctggaaggt aacaacgccg
     9061 aactcggtgc gaagaaactg gctgacagcg gcctgaatat tattgcagca aaaggtctga
     9121 cggatgcagc tcagcaggtt gttgccgcag tggaggggaa ataatgtcca ttttaattga
     9181 taaaaacacc aaggttatct gccagggctt tactggtagc caggggactt tccactctga
     9241 acaggccatt gcatacggca ctaaaatggt tggcggcgta accccaggta aaggcggtac
     9301 tacccacctc ggcctgccgg tgtttaacac cgtgcgtgaa gctgttgctg ccactggcgc
     9361 taccgcctct gttatctacg taccagcacc gttctgcaaa gactccattc tggaagctat
     9421 cgatgcaggc atcaaactga ttatcaccat cactgaaggc atcccgacgc tggatatgct
     9481 gacggtgaaa gtgaaactgg atgaagcagg cgttcgtatg atcggcccga actgcccagg
     9541 cgttatcacc ccgggggaat gcaaaatcgg tatccagccg ggtcacattc acaaaccagg
     9601 taaagtgggt atcgtttccc gttccggtac gctgacctat gaagcggtta aacagaccac
     9661 ggattacggt ttcggtcagt cgacctgtgt tggtatcggc ggcgacccga tccctggctc
     9721 taactttatc gatattctcg aaatgttcga aaaagatccg cagactgaag cgatcgtgat
     9781 gatcggtgag atcggcggta gcgctgaaga agaagcggct gcgtacatca aagagcacgt
     9841 caccaagcca gttgtgggtt acatcgctgg tgtgactgcg ccgaaaggca aacgtatggg
     9901 ccacgcgggt gccatcattg ccggtggtaa aggtactgcg gatgagaaat tcgctgctct
     9961 ggaagccgca ggcgtgaaaa ccgttcgcag cctggctgat atcggtgaag cactgaaaac
    10021 tgttctgaaa taaatatctg taataagaaa tagccctcgc cgcttccccc tacaggaagg
    10081 gcgaagggct gtcggtttcg acatggttgg ccatcttatg atggcctttt ttatgcttat
    10141 cgcggcgatt ttcgctgcgc tatcagggta aatttatagt catcagtatt aaaagcgttg
    10201 cggctatatt caaacacctg gccatcaact aaatatccac gcgatacttt ttcaagaatc
    10261 ggcttagtct ggctgatatt aagcagacgg ctcatctctt ccgtgggcat caggggaatt
    10321 atctcctgtt cgctacgatc gataaccatc ttcttcactt cttcaataaa gtgatatttc
    10381 gaattttcca tgacctgcca ggtgagatcc gggaataatg caagcggcat ccaggtttct
    10441 tccagcgcca ccggcttttg cttgcgatag cgcacgcgct tcacatgcca cacacgatcc
    10501 tgctcgctaa tttgcagctg ctgctgaaga aaattgtcag ccggaattac ttcaaatatc
    10561 aggacttcac tatgtgtttc gacgtgacgg tccgacagtt tttcatcaaa actggttaac
    10621 tgaaaaatat cgtaattgac ccgctcttct ttgacgtaag tcccgctgcc ctggatgctt
    10681 tcgaggatct gctgctcgac taactggcgc aaagcctggc gcaccgtaac ccggctgacg
    10741 ccaaactctg tttgtaatgc cgattcagtg ggtaacgcat cgccaggttt tagctcgccg
    10801 cacgtaatct gctcacgaat gcgatcggca atctgccggt ataagggttt gtgtcccatt
    10861 tctagtatct cattaatacg aatttagcca ttatgctcga taaattcatc ttgtaaataa
    10921 tacaaattta atacaaatag tatcttgtaa gtgaaagtga tcacgtaaat gtattgtttt
    10981 atcaggcaca cttgcgcgac tataaaaatg atcaagtgag aaccatgatg aacctgacga
    11041 ctctgaccca ccgcgatgcg ctgtgtctga atgtgcgctt taccagccgt gaagaggcca
    11101 tccacgcgtt gactcaacgt cttgctgctc tggggaaaat ttccagtact gagcaatttc
    11161 tggaagaagt gtattgccgt gaaagcctcg gcccgaccgc cttaggtgaa gggctggccg
    11221 tgccgcatgg caaaactgct gcggtaaaag aagcggcgtt tgcggtcgcc acactcagcg
    11281 agccgcttca gtgggaaggc gtggatggcc cggaagcggt tgatttagtg gtattgctgg
    11341 ccattcctcc caatgaagca ggtactacgc atatgaaact gcttaccgcg ctgaccacgc
    11401 gcctggcgga tgatgagctc agggcgcgta ttcagtcggc aacgacgccc gatgaattgc
    11461 tctcggcgct ggatgacaag ggggacgcgc aacatactgc ctctttttct tatgcgccaa
    11521 ctatcgtctg cgtaacggcc tgcccggcgg gtattgctca cacctacatg gctgcggaat
    11581 atctggaaaa agccggacgc aaactcggcg tgaatatcta cgttgaaaag caaggtgcta
    11641 acggtattga agagcgttta acggcggagc aactcaatag tgcaaccgcc tgtatttttg
    11701 cggcggaagt cgctatcaag gagagcgagc gttttaacgg cattcccgcg ctttctgtgc
    11761 ctgttgccga gccgattcgc catgcagaag cgttgattca acaagcgctt accctcaagc
    11821 gtagcgatga gacgcgtacc gtacagcaag atacgcaacc ggtgaaaagt gtcaaaacgg
    11881 agctgaagca ggcactgttg agcgggatct ctttcgccgt gccgctgatt gtcgcgggtg
    11941 gcacggtgct ggcggtcgcg gtattactgt cgcaaatctt cgggctacaa gatttgttta
    12001 atgaagaaaa ctcctggttg tggatgtacc gcaagctggg cggcgggatg ctcgggattt
    12061 tgatggtacc ggtgctcgca gcctataccg cctattcact ggctgataaa ccggcgttag
    12121 cgccaggctt tgcggcaggg cttgccgcca acatgattgg ttccgggttt ctcggcgcgg
    12181 tcgttggcgg attgatagcc ggttatctga tgcgctgggt gaaaaatcat ctacgtctta
    12241 gcagtaaatt caacggattc ctgactttct atctctaccc ggtgctcggt acgttggggg
    12301 cgggtagtct gatgctgttt gtagtagggg aacctgtcgc atggatcaat aactcgctta
    12361 ccgcctggct gaacggtctg tcaggaaata acgcactgtt gttgggcgcc attctcggtt
    12421 ttatgtgttc ctttgacctt ggtgggccag tgaacaaagc cgcttatgca ttctgtctgg
    12481 gggcaatggc gaacggcgtt tacggcccgt atgccatttt cgcctccgtc aaaatggttt
    12541 cggcatttac cgtaaccgct tccacgatgc tcgcaccgcg cctgtttaaa gagtttgaaa
    12601 ttgaaaccgg gaaatccacc tggctgttag gattggcagg tatcaccgaa ggggcgatcc
    12661 cgatggcgat tgaagatccg ctgcgggtta ttggttcgtt tgtgctggga tctatggtaa
    12721 cgggcgctat cgtcggtgcg atgaatatcg gcctttcgac acccggtgcc ggcattttct
    12781 cgctcttttt acttcatgat aatggcgcgg gtggtgttat ggcggcaatt ggttggtttg
    12841 gtgcggcgtt ggtgggggct gcaatctcga ctgcaattct cctgatctgg cggcatcatg
    12901 cggtaaagca tggaaactat ctgactgatg gcgtaatgcc ataaacaaaa aggaaacgac
    12961 gatgaaagcg gtatctcgcg ttcacatcac cccgcatatg cactgggatc gggagtggta
    13021 tttcaccaca gaagagtctc gcattctgct ggtcaataat atggaagaga tcctgtgcag
    13081 actggaacag gacaacgaat acaaatatta cgtactcgac gggcaaacgg cgatcctcga
    13141 agattatttc gctgtcaaac cggagaacaa agatcgtgtg aagaagttgg tcgagggcgg
    13201 gaaattaatt atcggtccct ggtataccca gaccgatacc acgattgttt ctgctgaatc
    13261 catagtccgt aatctgatgt acggaatgcg tgactgcctc gcgtttggcg agccgatgaa
    13321 aataggttat ttaccggatt cctttggcat gtccgggcaa ctgccacata tatacaatgg
    13381 atttggcatt acccgcacca tgttctggcg cggatgttct gagcgccacg gtactgataa
    13441 aaccgagttt ttgtggcaaa gcagtgacgg tagcgaagtg acggcgcagg tgctgccgct
    13501 gggctacgcc atcggtaagt acttacctgc ggacgaaaat ggattacgta aacgcctcga
    13561 cagttatttt gacgtgctgg aaaaagcgtc agtaaccaaa gaaattttat tgccgaatgg
    13621 tcatgaccag atgcccttgc agcaaaatat cttcgaagtg atggagaaac tgcgcgaaat
    13681 ttatcctcaa cgaaagtttg tgatgagccg ctttgaagag gtattcgagc agattgaagc
    13741 gcagcgtgaa agtctggcca tcctgaaagg tgaattcatc gatggcaaat atatgcgcgt
    13801 gcatcgcacc atcggttcca cgcgcatgga tatcaaaatt gcccacgcac gcattgaaaa
    13861 taagattgtt aatctgctgg aaccgttggc aacgctggcc tggactttag gttttgatta
    13921 tcaccacggc ttgctggaga agatgtggaa agaaatttta aaaaaccacg cccatgacag
    13981 catcggctgc tgctgcagtg ataaagttca tcgcgagatt gtcgcccgct tcgaactggc
    14041 agaagacatg gcggataatc taattcggtt ctatatgcgc aaaatagcag acaacatgcc
    14101 gcagagcgac gccgacaaac tcgtcctgtt taacctgatg ccctggccgc gtgaagaagt
    14161 tatcaacaca actgttcgac tgcgcggtag ccagtttaat ttgcgagacg gtcgcggtca
    14221 gcctgtgcca tattttattc gacatgcccg tgagatcgat cccggcctta ttgatcggca
    14281 aatagttcat tacggcaatt atgatccctt tatggagttt gatattcaga tcagccaaat
    14341 tgtcccttct atggggtatc gcacgctcta tatagaagcg aatcagcctg gcaatgtggt
    14401 cacgccaaaa agtaaaacag agggaatact ggaaaatgct ttctggcaaa ttgcactcaa
    14461 tgaggatggt tcacttcgtc tggttgataa agatagtggc gtgcgctatg accgggtgtt
    14521 tcaaattgaa gaaggttccg atgatgggga tgaatatgat tattcgccag caaaagaaga
    14581 gtgggccatt acctcagcga atgcgaaacc gcaatacgat attattcatg aagcctggca
    14641 gagtagggcc ataattcgct atgaaatagc ggtgccgcgt aatctgtcag agcgtcgcgc
    14701 aaagcaatgt tctggcaggg taggggtgga gacggtgatc actcttagcc ataacagcag
    14761 acgcattgat gcggatatta atcttgataa ccaggccgat gatcaccgta ttcgtgtatt
    14821 gattcccacg ccttttaaca cagatgttgt tcttgcggat acgcagtttg gttcattgac
    14881 gcggccagtg aaggattgcg caatgaatgt ctggcagcaa gaaggctgga aagaagcgcc
    14941 ggttccggta tggaatatgc tcaactatgc tgtcttacag gaagggcgta acggcatagc
    15001 cgtttttagc gaaggattac gtgaatttga agtcattgga gaagagaata aaacctttgc
    15061 tattacgttg ttgcgtggtg tgggattact gggaaaagaa gatttacttt taaggccagg
    15121 gcgtccttca ggaattaaaa tgcctgtgtc cgattcacaa ttacgcggat cattttcctg
    15181 tcgtctgagt ttatttagtt atatcggtac tcccgtaact gccggtgtag cccagcaggc
    15241 acgcgcatgg ctgacgccag ttcagtgtta taacaaaatc ccgtgggatg cgatgaagct
    15301 taataaagcc aaattcaatg tgccggaaag ctatagtttg ctgaaaatgc caccagtggg
    15361 atgtctgatc agcgcgctaa aaaaagcaga agacagacag gaattaatat tgcggctgtt
    15421 taatccggct gaattgacaa gctgtgatgc gaccgtcgcg tttagcagga aggtgatgac
    15481 gtgtacagaa accatgatga atgaacgctt taatacagaa aaaaatgagg ttttaaaaga
    15541 attagctctc tttttaccgg gacagtcgcg gacatt
//
LOCUS       DAHHTG010000101        15380 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.102, whole genome shotgun sequence.
ACCESSION   DAHHTG010000101 DAHHTG010000000
VERSION     DAHHTG010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15380)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15380)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15380
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.102"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            complement(100..768)
                     /gene="artM"
                     /locus_tag="NBI74_003299"
     CDS             complement(100..768)
                     /gene="artM"
                     /locus_tag="NBI74_003299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtM"
                     /protein_id="HCD1933583.1"
                     /translation="MFEYLPELMKGLHTSLTLTVASLIVALILALIFTIILTLKTPVL
                     VWLVRGYITLFTGTPLLVQIFLIYYGPGQFPTLQEYPALWHLLSEPWLCALIALSLNS
                     AAYTTQLFYGAIRAIPEGQWQSCSALGMSKKDTLAILLPYAFKRSLSSYSNEVVLVFK
                     STSLAYTITLMEVMGYSQLLYGRTYDVMVFGAAGIIYLVVNGLLTLMMRLIERKALAF
                     ERRN"
     gene            complement(768..1484)
                     /gene="artQ"
                     /locus_tag="NBI74_003300"
     CDS             complement(768..1484)
                     /gene="artQ"
                     /locus_tag="NBI74_003300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtQ"
                     /protein_id="HCD1933584.1"
                     /translation="MNEFFPLASAAGMTVGLAVCALIVGLALAMFFAVWESAKWRPVA
                     WAGSALVTILRGLPEILVVLFIYFGSSQLLLTLSDGFTINLGFVQIPVQMDIENFDVS
                     PFLCGVIALSLLYAAYASQTLRGALKAVPVGQWESGQALGLSKSAIFFRLVMPQMWRH
                     ALPGLGNQWLVLLKDTALVSLISVNDLMLQTKSIATRTQEPFTWYIVAAAIYLVITLL
                     SQYILKRIDLRATRFERRPS"
     gene            complement(1491..2222)
                     /gene="artJ"
                     /locus_tag="NBI74_003301"
     CDS             complement(1491..2222)
                     /gene="artJ"
                     /locus_tag="NBI74_003301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter substrate-binding
                     protein"
                     /protein_id="HCD1933585.1"
                     /translation="MKKVLIAALIAGFSLSATAAETIRFATEASYPPFESIDANNQIV
                     GFDVDLAQALCKEIDATCTFSNQAFDSLIPSLKFRRVEAVMAGMDITPEREKQVLFTT
                     PYYDNSALFVGQQGKYTSVDQLKGKKVGVQNGTTHQKFIMDKHPEITTVPYDSYQNAK
                     LDLQNGRIDSVFGDTAVVTEWLKDNPKLAAVGDKVTDKDYFGTGLGIAVRQGNTELQQ
                     KFNTALEKVKKDGTYETIYNKWFQK"
     gene            complement(2240..2968)
                     /gene="artP"
                     /locus_tag="NBI74_003302"
     CDS             complement(2240..2968)
                     /gene="artP"
                     /locus_tag="NBI74_003302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter ATP-binding protein
                     ArtP"
                     /protein_id="HCD1933586.1"
                     /translation="MSIQLNGINCFYGAHQALFDITLDCPQGETLVLLGPSGAGKSSL
                     LRVLNLLEMPRSGTLNIAGNHFDFTKTPSDKAIRDLRRNVGMVFQQYNLWPHLTVQQN
                     LIEAPCRVLGLSKDQALARAEKLLERLRLKPYSDRYPLHLSGGQQQRVAIARALMMEP
                     QVLLFDEPTAALDPEITAQIVSIIRELAETNITQVIVTHEVEVARKTASRVVYMENGH
                     IVEQGDASCFTEPQTEAFKNYLSH"
     gene            complement(3186..3701)
                     /locus_tag="NBI74_003303"
     CDS             complement(3186..3701)
                     /locus_tag="NBI74_003303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCD1933587.1"
                     /translation="MRYSKLTMLIPCALLLSACTTVTPAYKDNGPRTGSCVQGGPDSV
                     AQQFYDYRIQHRSNDITALRPYLSDKLATLLSDASRDNSHRELLSSDPFSSRTTLPDS
                     AHVASASTIPNRDARNIPLRVDLKQGDQGWQDEVLMIQEGQCWVIDDVRYLGGSVHAT
                     AGTLRQSIENR"
     gene            3827..4150
                     /locus_tag="NBI74_003304"
     CDS             3827..4150
                     /locus_tag="NBI74_003304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HCD1933588.1"
                     /translation="MQFSTTPTLEGQTIVEYCGVVTGEAILGANIFRDFFAGIRDIVG
                     GRSGAYEKELRKAREIAFEELGSQARALGADAVVGIDIDYETVGQNGSMLMVSVSGTA
                     VKTRR"
     gene            4147..4977
                     /gene="amiD"
                     /locus_tag="NBI74_003305"
     CDS             4147..4977
                     /gene="amiD"
                     /locus_tag="NBI74_003305"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiD"
                     /protein_id="HCD1933589.1"
                     /translation="MRRVFWLVAAALLLAGCTGEKGIVEKEGYQLDTRRQAQAAYPRI
                     KVLVIHYTADDFDSSLATLTDKQVSSHYLVPAVPPRYNGKPRIWQLVPEQELAWHAGI
                     SAWRGATRLNDTSIGIELENRGWQKSAGVKYFAPFEPAQIQALIPLAKDIIARYHIKP
                     ENVVAHADIAPQRKDDPGPLFPWQQLAQQGIGAWPDAQRVNFYLAGRAPHTPVETASL
                     LELLARYGYDVKPDMTPREQRRVIMAFQMHFRPTLYNGEADAETQAIAEALLEKYGQD
                     "
     gene            complement(4974..5987)
                     /locus_tag="NBI74_003306"
     CDS             complement(4974..5987)
                     /locus_tag="NBI74_003306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HCD1933590.1"
                     /translation="MKVLVTGATSGLGRNAVEFLCQKGISVRATGRNEAMGKLLEKMG
                     AEFVPADLTELVSSQAKVMLAGIDTLWHCSSFTSPWGTQQAFDLANVRATRRLGEWAV
                     AWGVRNFIHISSPSLYFDYHHHRDIKEDFRPHRFANEFARSKAASEEVINMLSQANPQ
                     TRFTILRPQSLFGPHDKVFIPRLAHMMHHYGSILLPHGGSALVDMTYYENAVHAMWLA
                     SQEACDKLPSGRVYNITNGEHRTLRSIVQKLIDELNIDCRIRSVPYPMLDMIARSMER
                     LGRKSAKEPPLTHYGVSKLNFDFTLDITRAQEELGYQPVLTLDEGIEKTAAWLRDHGK
                     LPR"
     gene            complement(6086..7516)
                     /locus_tag="NBI74_003307"
     CDS             complement(6086..7516)
                     /locus_tag="NBI74_003307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2867 domain-containing protein"
                     /protein_id="HCD1933591.1"
                     /translation="MPQRILVLGASGYIGQHLVHTLSQQGHQILAAARHVDRLAKLQL
                     ANVSCHKVDLNWPDNLPVLLQNIDTVYFLVHSMGEGGDFIAQERQVALNVRDALREVP
                     VKQVIFLSSLQAPPHEQSDHLRARQATADILREAGVPVTELRAGIIVGAGSAAFEVMR
                     DMVYNLPVLTPPRWVRSRTTPIALENLLHYLVALLDHPACEHRIFEAAGPEVLSYQQQ
                     FEHFMAVSGKRRWLLPIPLPTRWISVWFLNVITSVPPTTARALIQGLKHDLLADDTAL
                     RALIPQPLIAFDDAVRSTLKEEEKLVNSSDWGYDAQAFARWRPEYGYFAKQAGFTVKT
                     SASLQALWRVVNQIGGKERYFFGNILWQTRALMDLAIGHKLAKGRPEREYLQTGDAVD
                     SWKVIVVEPEKQLTLLFGMKAPGLGRLCFTLEDKGDYRTIDVRAFWHPHGMPGLFYWL
                     LMIPAHLFIFRGMAKRIARLAEQSTD"
     gene            complement(7527..8528)
                     /gene="ltaE"
                     /locus_tag="NBI74_003308"
     CDS             complement(7527..8528)
                     /gene="ltaE"
                     /locus_tag="NBI74_003308"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HCD1933592.1"
                     /translation="MIDLRSDTVTRPSRAMLEAMMAAPVGDDVYGDDPTVNALQDYAA
                     ELSGKEAAIFLPTGTQANLVALLSHCERGEEYIVGQAAHNYLFEAGGAAVLGSIQPQP
                     IDAAADGTLPLDKVAMKIKPDDIHFARTKLLSLENTHNGKVLPREYLKEAWEFTRERN
                     LALHVDGARIFNAVVAYGCELKEITQYCDSFTICLSKGLGTPVGSLLVGNRDYIKRAI
                     RWRKMTGGGMRQSGILAAAGMYALKNNVARLQEDHDNAAWMAEQLREAGADVMRQDTN
                     MLFVRVGEENAAALGEYMKARNVLINASPIVRLVTHLDVSREQLAEVAAHWRAFLAR"
     gene            complement(8565..10283)
                     /gene="poxB"
                     /locus_tag="NBI74_003309"
     CDS             complement(8565..10283)
                     /gene="poxB"
                     /locus_tag="NBI74_003309"
                     /EC_number="1.2.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone-dependent pyruvate dehydrogenase"
                     /protein_id="HCD1933593.1"
                     /translation="MKQTVAAYIAKTLESAGVKRIWGVTGDSLNGLSDSLNRMGTIEW
                     MSTRHEEVAAFAAGAEAQLSGELAVCAGSCGPGNLHLINGLFDCHRNHVPVLAIAAHI
                     PSSEIGSGYFQETHPQELFRECSHYCELVSSPEQIPQVLAIAMRKAVLNRGVSVVVLP
                     GDVALKPAPEGATTHWYHAPQPVVTPEEEELRKLAQLLRYSSNIALMCGSGCAGAHKE
                     LVEFAGKIKAPIVHALRGKEHVEYDNPYDVGMTGLIGFSSGFHTMMNADTLVLLGTQF
                     PYRVFYPTDAKIIQIDINPASIGAHSKVDMALVGDIKSTLRALLPLVEEKTERKFLDK
                     ALEDYRDARKGLDDLAKPSEKAIHPQYLAQQISHFAADDAIFTCDVGTPTVWAARYLK
                     MNGKRRLLGSFNHGSMANAMPQALGAQATEPERQVVAMCGDGGFSMLMGDFLSVVQMK
                     LPVKIIVFNNSVLGFVAMEMKAGGYLTDGTELHDTNFARIAEACGITGIRVEKASEID
                     EALQRAFSIDGPVLVDVVVAKEELAIPPQIKLEQAKGFSLYMLRAIISGRGDEVIELA
                     KTNWLR"
     gene            complement(10416..11384)
                     /gene="hcr"
                     /locus_tag="NBI74_003310"
     CDS             complement(10416..11384)
                     /gene="hcr"
                     /locus_tag="NBI74_003310"
                     /EC_number="1.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidoreductase"
                     /protein_id="HCD1933594.1"
                     /translation="MTMPTNQCPWRMQVHHITQETPDVWTISLICHDYYPYRAGQYAL
                     VSVRNSAETLRAYTISSTPGVSEYITLTVRRIDDGVGSRWLTRDVKRGDYLWLSDAMG
                     EFTCDDKAEDKFLLLAAGCGVTPIMSMRRWLAKNRPQADVQVIYNVRTPQDVIFADEW
                     RNYPVTLVAENNVTEGFIAGRLTRELLAGVPDLASRTVMTCGPAPYMDWVEQEVKTLG
                     VTRFFKEKFFTPVAEAATSGLKFTKLQPAREFYAPVGTTLLEALESNNVPVVAACRAG
                     VCGCCKTKVISGEYTVSSTMTLTDAEIAEGYVLACSCHPQGDLVFA"
     gene            complement(11396..13048)
                     /gene="hcp"
                     /locus_tag="NBI74_003311"
                     /gene_synonym="priS"
     CDS             complement(11396..13048)
                     /gene="hcp"
                     /locus_tag="NBI74_003311"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HCD1933595.1"
                     /translation="MFCVQCEQTIRTPAGNGCSYAQGMCGKTAETSDLQDLLIATLQG
                     LSAWAVKAREYGIINHDVDSFAPRAFFSTLTNVNFDSPRIIGYAREAIALREALKAQC
                     LAVDANARVDNPMADLQLVSDDLGELQRQAAEFTPNKDKAAIGENILGLRLLCLYGLK
                     GAAAYMEHAHVLGQYDNDIYAQYHKIMAWLGTWPADMNALLECSMEIGQMNFKVMSIL
                     DAGETGKYGHPTPTQVNVKATAGKCILISGHDLKDLYNLLEQTEGTGVNVYTHGEMLP
                     AHGYPELRKFKHLVGNYGSGWQNQQVEFARFPGPIVMTSNCIIDPTVGAYDDRIWTRS
                     IVGWPGVRHLDGEDFSAVIAQAQQMAGFPYSEIPHLITVGFGRQTLLGAADTLIDLVS
                     REKLRHIFLLGGCDGARGERHYFTDFATSVPDDCLILTLACGKYRFNKLEFGDIEGLP
                     RLVDAGQCNDAYSAIILAVTLAEKLGCGVNDLPLSLVLSWFEQKAIVILLTLLSLGVK
                     NIVTGPTAPGFLTPDLLAVLNEKFGLRSITTVEEDMKQLLSA"
     gene            complement(13192..14091)
                     /gene="lysO"
                     /locus_tag="NBI74_003312"
     CDS             complement(13192..14091)
                     /gene="lysO"
                     /locus_tag="NBI74_003312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lysine exporter LysO"
                     /protein_id="HCD1933596.1"
                     /translation="MFSGLLIILVPLIVGYLIPLRQKAALRVINQLLSWMVYLILFFM
                     GISLAFLDNLASNLLAILHYSAVSTTVILLCNIAALMWLERGLPWSNHHQQEKLPSRI
                     AMALESLKLCGVVVIGFAIGLSGLAFLQHATEASEYTLILLLFLVGIQLRNNGMTLKQ
                     IVLNRRGMIVAVVVVASSLIGGLINAFILDLPINTALAMASGFGWYSLSGILLTESFG
                     PVIGSAAFFNDLARELIAIMLIPGLIRRSRSTALGLCGATSMDFTLPVLQRTGGLDMV
                     PAAIVHGFILSLLVPILIAFFSA"
     gene            complement(14506..15201)
                     /gene="aqpZ"
                     /locus_tag="NBI74_003313"
     CDS             complement(14506..15201)
                     /gene="aqpZ"
                     /locus_tag="NBI74_003313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin Z"
                     /protein_id="HCD1933597.1"
                     /translation="MFRKLAAECFGTFWLVFGGCGSAVLAAGFPELGIGFAGVALAFG
                     LTVLTMAFAVGHISGGHFNPAVTIGLWAGGRFPAKEVVGYVIAQVVGGIVAAALLYLI
                     ASGKTGFDAAASGFASNGYGEHSPGGYSMLSALVVELVLSAGFLLVIHGATDKFAPAG
                     FAPIAIGLALTLIHLISIPVTNTSVNPARSTAVAIFQGGWALEQLWFFWVVPIVGGII
                     GGLIYRTLLEKRN"
BASE COUNT         3813 a         4089 c         4006 g         3472 t
ORIGIN      
        1 gacagcttag tgggttgtcc gtcttcataa taagaattta tgcactatgt aggccggata
       61 aggcgtccac gccgcatccg gcatcagcat gcagctacat taattccgcc gttcgaatgc
      121 cagtgcttta cgctcgatca gacgcatcat cagcgtcagc aggccgttga cgaccaggta
      181 aataatcccc gccgcgccaa ataccattac atcgtaggtg cgtccgtaca acaactggct
      241 gtatcccatc acttccatga gcgtaatggt gtaagccaga gaggtacttt tgaacaccag
      301 caccacttcg ttggaataag aagagagcga gcgtttaaac gcgtacggca gcaggatcgc
      361 cagcgtatct tttttgctca ttcccagggc gctacaggac tgccattgac cttccgggat
      421 cgcgcgaatc gcaccgtaaa acagctgcgt ggtgtacgcc gcactgttca gcgacaacgc
      481 aatcagcgca cataaccacg gttctgacaa caaatgccac agtgccggat actcctgcaa
      541 cgtcgggaac tggcccggcc cgtaataaat caggaagatc tgcaccagca gcggcgtacc
      601 ggtaaacagc gtgatatagc cccgtaccag ccacaccagc accggcgttt tcagcgtcag
      661 gatgatggta aaaatcaatg ccagaatcag tgccacaatc agcgaggcaa cggttagcgt
      721 caggctggtg tgcagccctt tcatcagttc gggtaaatac tcaaacatta gctgggcctc
      781 cgctcaaaac gtgtcgcgcg caggtcaatg cgtttgagaa tgtactgact gagcagggtg
      841 atcaccaggt aaatcgccgc cgccacaatg taccaggtaa acggttcctg ggtacgggta
      901 gcgatgcttt tggtttgtag cattaaatca ttcacactaa tcaaactgac cagcgcggta
      961 tctttcagca gcaccagcca ctggttaccg agacccggca gcgcatgacg ccacatctgc
     1021 ggcatcacca gacggaaaaa gatagccgat ttcgacagcc ccagcgcctg accggattcc
     1081 cactgcccca ccggcaccgc ttttaatgca ccacgcagcg tttgcgaggc ataggcggca
     1141 tacagcagtg acagagcgat gacaccacaa aggaacgggc taacgtcgaa gttctcaatg
     1201 tccatctgca ccgggatctg cacgaaccca agattgatag tgaagccatc cgaaagcgtc
     1261 agcagcagct gcgaggagcc aaaatagata aacagcacca ccagaatttc tggcaggcca
     1321 cgcagaatgg ttaccagcgc tgaacctgcc cacgcgacag gacgccattt cgcagactcc
     1381 cataccgcaa agaacatcgc cagcgccagc ccgacaatca atgcacaaac ggcaaggccg
     1441 acggtcatcc cggcggcgct tgctaaagga aaaaattcat tcatcaggaa ttacttctgg
     1501 aaccatttgt tgtagatggt ttcgtaagtg ccatctttct tcactttttc cagcgcagtg
     1561 ttgaatttct gctgcagttc agtgttgccc tggcgtactg cgatgccgag gccagtaccg
     1621 aagtaatctt tatcggtcac tttatcgccc actgccgcca gtttcgggtt atctttcagc
     1681 cattcagtta ccactgcggt gtcaccaaat acgctgtcga tacgaccgtt ttgcagatcc
     1741 agttttgcgt tctggtaact gtcatatggc acggtagtga tttccggatg cttatccata
     1801 atgaacttct ggtgtgtcgt cccgttctga acgccaactt ttttgccttt cagctgatca
     1861 acactggtgt atttgccttg ctgacccaca aacagggcag agttgtcata gtacggggtg
     1921 gtaaacagca cctgcttttc acgctccgga gtgatatcca tgcccgccat cactgcttct
     1981 acgcgacgga atttcaggct tgggatcagg ctgtcaaacg cctggttaga gaaagtgcag
     2041 gttgcatcaa tctctttaca caatgcctgc gccaggtcga catcaaaacc aacgatctgg
     2101 ttgtttgcat caatcgattc aaacggagga taggaggctt cggtggcaaa acgaatggtt
     2161 tcggctgctg tggcggaaag actaaaacct gcaattaacg cggcaatcag aacttttttc
     2221 attgttgtta tcccgaatct tagtgagaga gatagttttt aaatgcttcg gtttgcggct
     2281 cggtaaagca gctcgcgtcg ccttgttcta cgatatgacc gttttccata tacaccactc
     2341 ggctggcggt tttacgcgcc acttcaactt cgtgggtgac gatcacctgg gtaatattcg
     2401 tttctgccag ctcacgaatg atgctgacga tttgtgccgt aatttcgggg tccagtgcgg
     2461 cggtcggttc atcgaacagc agtacctgcg gttccatcat caacgcacgg gcaatagcaa
     2521 cacgctgctg ctgaccacca gaaagatgca gcgggtaacg atcgctataa ggtttaagac
     2581 gcagacgttc cagcagtttt tctgcacggg ccagcgcctg atctttactc agccccagta
     2641 cacggcaggg cgcttcaatc aggttttgct gcacggtcag atgcggccac aggttgtatt
     2701 gctgaaacac catgccaacg ttacgacgca aatcgcgaat cgctttgtca gagggggttt
     2761 tggtgaaatc gaaatggttg cctgcaatgt tgagcgtacc ggagcgcggc atctcaagca
     2821 gattgagtac acgcagcagc gagcttttac ccgcgccgct ggggccaagt aacaccagcg
     2881 tttcgccctg cgggcaatcc agcgtgatat cgaacagcgc ctgatgcgcg ccgtagaagc
     2941 aattaatgcc gtttaattga atactcattg acactcgtat actggcagtc tgatagctat
     3001 tgaggtcgaa gatagtacct ttgacagaat aattatgcaa tatttctgct ttaaaagtta
     3061 aaagcaaagc gcattattca ataaacatag cacaaaataa cgggggcggt ggtcggcgag
     3121 cataaatgtc ggcattcctc acgaaatgcc ggacaattta cggggtttat tggttgatca
     3181 agacgttagc gattctcgat ggattgacgg agcgtacccg ccgtggcatg aacgctaccg
     3241 cctaagtagc gcacatcgtc gatgacccaa cactggcctt cctgaatcat taacacttca
     3301 tcctgccagc cctggtcacc ctgcttgaga tccacgcgca atggaatgtt acgggcatca
     3361 cgattaggga tagtcgatgc actggctacg tgggcgctgt ctggcaaggt ggttcgactg
     3421 gagaacggat cgctgcttaa caattcacga tgactattat cgcggctggc atcgctaagt
     3481 agggttgcca gtttgtcact caaatagggg cgcagagcgg taatgtcatt gctgcgatgt
     3541 tgaatgcggt agtcatagaa ctgttgagcc acactatcag gccccccctg gacacaggaa
     3601 ccggtgcggg ggccgttatc tttataagct ggcgtgacgg tggtacaggc actgagtagc
     3661 agtgcgcaag ggataagcat ggtcaatttg ctatagcgca tgatgatttc cttataagcg
     3721 aacaccttga aagcgttcta cgataataat gatatccttt caataatagc gtatcagtcc
     3781 gataatgctt tgaagatcga aggcttagca aacaaggaga tcgatcatgc aattttctac
     3841 aaccccaact ctggaaggcc agaccatcgt tgaatattgc ggtgtggtga ctggcgaagc
     3901 gattttaggc gcgaatattt tccgtgattt ctttgccggt atccgcgata tcgttggcgg
     3961 acgctccggt gcctacgaaa aagaactgcg taaagcgcgg gagattgcct ttgaggagct
     4021 cggctcccag gcgcgagcgc tgggggctga tgccgtcgtc ggtattgata tcgactacga
     4081 aacggtcggg caaaacggca gtatgctgat ggttagcgtc agcggtacgg cggtgaaaac
     4141 gcgtcgatga gaagagtctt ctggctggtc gctgccgctc tgttactggc agggtgcaca
     4201 ggcgaaaaag gcattgtcga gaaagaggga tatcagcttg atacccgacg ccaggcgcag
     4261 gcggcgtatc cgcgcattaa agtgctggtg atccactaca ccgcagatga ttttgatagc
     4321 tcgctggcga cactgaccga taagcaggtc agctcgcatt atctggtccc tgcggtgcca
     4381 ccgcgataca acggtaaacc gcgcatctgg caactggtgc cggaacaaga actggcctgg
     4441 catgcgggga ttagcgcctg gcgcggggca acgcgtctta acgacacctc tattggcatt
     4501 gagctggaaa accgtggctg gcaaaaatcg gcgggagtga aatattttgc cccgtttgaa
     4561 ccggcacaga ttcaggcgct tattccgctg gcgaaagata ttattgcccg ttatcacatc
     4621 aagccggaaa acgtagtagc acatgcggat attgcgccac agcgcaaaga cgatccgggg
     4681 ccattatttc cctggcagca actggcacag caggggattg gtgcctggcc ggatgcgcag
     4741 cgggtgaact tttaccttgc cgggcgcgcg ccgcacacac cggtagagac cgcgtcattg
     4801 ctggagcttt tggcgcgtta tggttatgac gtaaagcctg atatgactcc gcgcgagcag
     4861 cggcgtgtga ttatggcgtt ccagatgcat ttccgcccga cgttatataa cggcgaagcg
     4921 gatgcagaaa ctcaggcgat tgccgaagcg ttgctggaga aatacgggca ggattagcgc
     4981 ggcagttttc cgtggtcgcg cagccaggcg gcagttttct cgataccttc atccagggtg
     5041 aggactggct gataacctaa ctcttcctgc gcacgcgtaa tatccagcgt aaagtcaaaa
     5101 ttcaacttgg agacgccgta gtgggtcagc ggcggctctt ttgctgactt gcggcctaaa
     5161 cgctccatgc tgcgggcgat catatccagc atcgggtaag gaaccgaacg aatacgacag
     5221 tcaatattca actcgtcgat cagcttctgc acgatgctgc gcagtgtgcg atgctcgccg
     5281 ttggtgatgt tgtacacacg cccggaaggt agcttatcgc acgcttcctg gcttgccagc
     5341 cacatggcgt gcacggcatt ttcatagtag gtcatatcga ccagcgcact gccgccatgc
     5401 ggtaacagaa tactgccgta gtgatgcatc atatgcgcca gacgagggat aaagacttta
     5461 tcgtgcggtc cgaacagact ttgtgggcgc agaatagtaa agcgcgtttg tggattcgcc
     5521 tgcgaaagca tattgatcac ttcttcgctg gctgctttgc tgcgggcaaa ctcgttggcg
     5581 aagcggtgag ggcgaaaatc ttctttaata tcgcgatggt ggtgataatc gaagtacagg
     5641 gagggagaag agatatgaat aaagttacgc acaccccaag cgacagccca ttcacccagg
     5701 cggcgagtgg cgcgaacgtt agccagatcg aaagcctgtt gtgtccccca gggcgaggta
     5761 aagctggagc agtgccacag cgtatcaatg cccgcgagca tcactttagc ttgtgatgag
     5821 accaactcgg tcagatccgc cggaacaaac tctgcgccca ttttttccag caatttaccc
     5881 attgcctcgt tgcgaccggt cgctcgcacg ctgatgcctt tctggcataa aaactctacc
     5941 gcgtttcgac ctaagccgct ggtggcgccg gtaaccagta ccttcatatc aatccactgt
     6001 tgttgagaaa atgacgtgcg cattcttccg tgatttcccc cttgatgcaa tgggaaacat
     6061 gaaagaataa cgcaggtttt gtcgattaat ctgtgctttg ttctgccagt ctggcgattc
     6121 gttttgccat tccgcgaaaa ataaacagat gcgcggggat catcaataac cagtaaaaca
     6181 gccccggcat accgtgcgga tgccagaaag cacggacatc gattgtacga tagtcgcctt
     6241 tatcttccag ggtaaaacag agtcgtccca gccccggcgc tttcatgcca aataacaacg
     6301 taagttgttt ttccggttca acgacaatga ctttccagct atccacagca tcgccggtct
     6361 gtaaatattc gcgctccggg cggccttttg ccagcttatg accgatcgca aggtccatca
     6421 acgcccgtgt ctgccacaaa atattgccaa agaaataacg ctctttaccg ccgatttgat
     6481 tgaccacccg ccatagcgcc tgaaggctgg cagacgtttt aacggtaaac cccgcctgtt
     6541 tggcaaaata accgtattct ggtcgccagc gagcaaaagc ctgcgcgtca tatccccagt
     6601 cgctggagtt gaccagtttt tcttcctctt tcaacgtgct acgtaccgcg tcatcgaaag
     6661 cgatcagcgg ttgtggaatg agtgcacgta acgcggtgtc atccgccagc agatcgtgtt
     6721 tcagcccctg aatcaacgct ctggcggtgg tgggcggtac tgaagtaatc acattaagaa
     6781 accacaccga aatccagcgg gtggggaggg ggatggggag caaccagcgg cgcttaccgc
     6841 tcaccgccat aaaatgttca aactgttgct gataactgag cacttctggc ccagcggctt
     6901 cgaagatgcg atgttcgcaa gccggatgat ctaacagcgc cacaagatag tgcagcaggt
     6961 tttccagcgc gatgggcgta gtgcgtgaac gtacccagcg tggtggcgtt aatactggca
     7021 ggttgtaaac catatcgcgc atgacttcga atgccgctga acccgcgcca acgataattc
     7081 cggcacgtag ttcggtcacc gggacacccg cttcacgtaa aatatccgcc gtggcctgac
     7141 gagcacgcag atgatcagat tgttcatgag gcggtgcctg caatgaactg agaaagatga
     7201 cttgctttac cggtacttcg cgcagcgcat cgcggacgtt gagagccacc tggcgctcct
     7261 gagcgataaa atcaccacct tcgcccatgc tgtgcaccag aaaatagaca gtatcaatat
     7321 tctgcaacag gaccggaagg ttatccggcc agttgagatc gactttatgg caactgacat
     7381 tagccagttg cagttttgca agtctgtcga cgtgacgtgc cgccgccagg atctgatgcc
     7441 cttgctggct gagtgtgtgc accagatgtt gaccaatgta gccactggca ccgagaacta
     7501 aaatgcgttg cggcacgtct ctctccttaa cgcgccagga acgcacgcca gtgggcggca
     7561 acttccgcca gttgttcacg cgagacgtca agatgggtca ccaggcggac aatcggcgag
     7621 gcgttaatca gcacgtttct cgctttcatg tattcgccta acgcggcagc attttcttcc
     7681 ccgacgcgaa caaacagcat attggtgtcc tgacgcatca catccgcgcc tgcttcacgc
     7741 aactgctccg ccatccaggc ggcgttatcg tggtcttcct gcaaacgtgc gacgttattt
     7801 ttcagcgcat acatcccggc ggcagccaga atgccggact gacgcatccc gccacctgtc
     7861 attttccgcc agcgaatggc acgtttaatg taatcacgat taccgacgag taatgaaccg
     7921 actggcgtcc caagaccttt cgacaggcaa atggtgaacg agtcacaata ttgcgtgatc
     7981 tctttcagtt cgcagccgta agccaccacg gcattaaaaa tgcgcgcacc gtcaacgtgc
     8041 agcgccagat tgcgctcgcg ggtaaattcc catgcttctt tcaggtattc acgcggcagc
     8101 actttgccgt tgtgggtgtt ttccagactg agtaatttgg tgcgggcgaa atggatatcg
     8161 tcgggtttga ttttcatcgc cactttatcc agcggtagcg taccgtcggc agctgcgtcg
     8221 atgggttgcg gctgaatact gcccagtact gctgcgccac cggcttcaaa cagatagtta
     8281 tgcgcggcct gaccgacgat atactcttcg ccgcgttcgc agtgactgag cagagcgacc
     8341 aggttggcct gagtgccggt aggcagaaaa atggcggctt ctttaccgga aagctctgct
     8401 gcatagtcct gcagagcatt aacggtaggg tcgtctccat aaacgtcgtc cccaaccggg
     8461 gcggccatca tcgcttcgag catggcgcgg ctcggtcggg taacggtatc actgcgtaaa
     8521 tcaatcatgg catgtcctta ttatgacggg aaatgccacc ctttttacct tagccagttc
     8581 gttttcgcca gttcgatcac ttcatcaccg cgcccgctga tgattgcgcg cagcatatac
     8641 aggctgaaac ctttggcctg ttcgagtttg atctgcggtg gaatggctaa ttcttctttg
     8701 gcgaccacca catccaccaa caccggaccg tcgatggaga aggcgcgttg cagggcttca
     8761 tcgatttcag acgctttttc tacgcggata cccgtaatgc cgcaggcttc ggcaattcgg
     8821 gcaaagttgg tgtcgtgtag ttcggtgccg tcagtcaaat agccaccagc tttcatctcc
     8881 atcgccacaa agcccagcac gctgttatta aagacgataa ttttcactgg cagcttcatt
     8941 tgcactaccg agaggaaatc gcccatcaac atgctaaaac cgccatcgcc gcacatggcg
     9001 accacctggc gttctggctc ggttgcctgc gcgcccagcg cctgcggcat ggcgttagcc
     9061 atcgaaccat ggttaaacga acctaacagg cgacgcttgc cgttcatttt tagataacgt
     9121 gccgcccaca ccgttggcgt accaacgtca caggtgaaaa tagcgtcatc ggcggcaaaa
     9181 tgactaattt gctgcgccag atattgcggg tgaatggctt tctcgctcgg tttagctaaa
     9241 tcgtccagcc ctttgcgggc gtcgcggtaa tcttccagcg ccttatccag aaacttgcgt
     9301 tcggtttttt cttccaccag tggcagcaat gcgcgcaggg ttgatttgat atcgccgacc
     9361 agcgccatat ctaccttgct atgcgcgccg atgctggctg ggttgatatc aatctgaata
     9421 attttggcat cggtcgggta gaacacacgg tagggaaact gagtaccgag cagcactaac
     9481 gtatcggcgt tcatcatggt atggaaacct gacgaaaagc cgattaatcc cgtcattcca
     9541 acatcatatg gattatcgta ttcgacatgt tctttaccgc gcagggcatg gactataggc
     9601 gctttaattt tcccggcaaa ctcaactaac tctttatgcg cccccgcaca gccgctgcca
     9661 cacatcaggg cgatattgct ggaataacgc agcagttgcg ccagtttgcg taactcttct
     9721 tcttccggtg ttactaccgg ctgtggcgca tgataccagt gggtagttgc cccttctggc
     9781 gcaggtttta acgccacatc gcccggtaac acaacaacgg aaacgccacg gttaagcacc
     9841 gctttgcgca tagcaattgc cagcacttgt gggatctgct ctgggctgga aaccagctcg
     9901 caatagtgac tacattcgcg gaatagctct tgtgggtggg tttcctggaa atagccgctg
     9961 ccaatttcgc tggagggaat atgagcggca atcgccagta ccggaacgtg attgcggtgg
    10021 caatcgaaca ggccgttgat taagtgcagg ttgccgggac cgcacgatcc ggcacagacc
    10081 gccagttctc cgctaagttg tgcttcagcg ccagcggcaa atgccgccac ttcttcatgc
    10141 cgggtggaca tccactcgat ggtgcccatg cgattaagac tgtcactaag accgttcaga
    10201 gagtcgcccg tgactcccca gatgcgtttc acgcctgccg attcgagtgt tttggcgata
    10261 taagctgcaa ccgtttgttt catggttctc catctcctga atgtgataac ggtaacaagt
    10321 ttagttcatc tgacggaggg ggaagggatg ggagaaaaag gaggcactaa cggttaaata
    10381 gcccgatgaa aggaatatca tcgggcatca ggcgattatg cgaaaaccaa atccccctgc
    10441 ggatggcatg agcaggccag tacgtaacct tcagcgattt cggcgtcggt cagcgtcatt
    10501 gtgctgctca ccgtatattc accggaaatc actttcgtct tacagcagcc gcaaacaccc
    10561 gcacggcagg cggcgacaac cggaacgtta ttgctttcca gcgcctccag tagcgtggtg
    10621 ccaaccggag cgtaaaattc tcgtgccggt tgcagtttgg tgaatttcag accgctggtc
    10681 gccgcttccg ctactggggt gaagaatttc tctttaaaga aacgcgtcac gccgagcgtt
    10741 ttcacttcct gctctaccca atccatatat ggagcagggc cacaggtcat cacggtacgt
    10801 gaggctaaat cagggacacc tgccagcagt tcgcgagtga gacgaccagc gataaagcct
    10861 tcggtaacgt tattttccgc caccagcgtt accggatagt tacgccactc atcggcgaaa
    10921 atcacatcct gcggcgtacg cacgttgtag atcacctgca catcggcctg tggacggttc
    10981 ttcgccagcc agcgacgcat cgacataatc ggcgtgacgc cgcagcctgc cgccagcaac
    11041 aggaatttat cttctgcttt atcgtcgcag gtaaattccc ccatcgcgtc cgaaagccag
    11101 agataatcac cgcgttttac atcgcgcgtc agccaccggg agccgacacc gtcatcaatc
    11161 cgccgcacag tcagggtgat gtattcactc acgcctggcg tggaggaaat ggtgtaagca
    11221 cgcagcgttt ctgctgagtt tcgcacgctg accagcgcat attgcccggc gcgatacggg
    11281 tagtaatcgt ggcaaatcag ggaaatcgtc cacacatccg gcgtttcttg cgtaatgtga
    11341 tgaacctgca tccgccacgg gcattgattc gttggcatcg tcatcgacaa actccttacg
    11401 cgctcaacag ttgcttcatg tcttcttcaa cagtggtgat agaacgcagg ccgaatttct
    11461 cgttcagcac cgccagcagg tctggtgtca ggaaaccagg tgcagtcggg ccggtgacga
    11521 tatttttcac acccagagaa agcagcgtca gcagaatgac aatcgctttc tgttcaaacc
    11581 aggagagcac cagcgacagc ggcagatcgt tgactccgca gcccagtttc tctgccagcg
    11641 ttaccgccag aataattgct gagtaagcat cgttacactg accagcatct accagacgcg
    11701 gcagaccttc gatatcgcca aactcaagtt tattaaagcg atatttaccg caagcgaggg
    11761 tcaggatcag gcagtcatcc ggcacgctgg tggcgaaatc ggtgaagtag tgacgctcgc
    11821 cgcgtgcgcc gtcacagccg ccaagcagga agatatgacg cagtttttca cggctcacca
    11881 gatcaatcag cgtatcggct gcgccaagca gtgtctggcg accaaagccc acggtgatca
    11941 ggtgcggaat ttcgctgtac gggaagcccg ccatctgttg cgcctgggca atgaccgcag
    12001 agaaatcttc gccatccaga tggcgcacac ccggccagcc aacgatgctg cgggtccaga
    12061 tacgatcgtc ataagcgcct acggttgggt cgatgatgca gttcgaggtc atcacgatgg
    12121 ggcctgggaa acgagcgaac tccacttgct gattctgcca gccgctgccg tagttaccga
    12181 ccagatgctt gaatttacgc agctccgggt aaccatgcgc aggcagcatt tcgccgtggg
    12241 tgtagacatt aacgcccgtg ccttcggtct gttccagcag gttgtagaga tctttgagat
    12301 cgtgaccgga aatcaggatg catttacctg ccgtcgcttt gacattgacc tgggttgggg
    12361 tcgggtgacc gtatttaccg gtttcgcctg cgtccagaat gctcatcact ttgaagttca
    12421 tctggccgat ttccattgaa cactcaagaa gcgcgttcat atcggcaggc caggtcccca
    12481 gccacgccat gattttatgg tactgggcat aaatatcgtt gtcgtattga ccgagaacat
    12541 gcgcgtgttc catataggcc gccgcacctt tcaggccata caggcacagc agacgcaggc
    12601 cgagaatgtt ttcgccaatc gccgctttat ctttgttagg ggtaaattct gctgcctgac
    12661 gttgcagctc gccgagatcg tcgctcacca gttgcaggtc agccatcggg ttatcgacgc
    12721 gggcgttggc atctacagcc aggcattgtg ctttcagcgc ctcgcgcagg gcaatcgctt
    12781 cacgagcgta gccgataata cgcggagaat cgaagttaac gttggtcagg gttgagaaaa
    12841 aggcacgtgg cgcaaagctg tctacatcgt ggttgatgat gccgtattca cgtgctttca
    12901 ctgcccaggc cgaaagccct tgcagcgtcg cgatgagtaa atcctgaagg tcagaggttt
    12961 ccgccgtttt accacacatc ccctgcgcgt atgagcagcc gtttcctgcc ggagtacgga
    13021 tagtttgttc acattgcaca caaaacatga tcacaccttt taaagttata tttaatatac
    13081 atgtttaagg ttaagacgct taacgcgggg ataaaagaga tttttaatgc aactttaagg
    13141 gagattgatt tagcgcaatt ttggcggcag agatctaccg ccagagaggt attatgcaga
    13201 gaaaaaggcg atgagaatcg gcactaacag actaagaata aaaccgtgaa caatcgccgc
    13261 cgggaccata tccagcccgc ctgtacgttg aagaacgggc agggtgaaat ccatcgacgt
    13321 cgcaccgcat aagcccagtg cagtagagcg gctacggcga atcagcccag ggattaacat
    13381 aatagcaatc agctcacggg ccagatcatt aaaaaacgcc gcgctcccga ttaccggacc
    13441 aaaagattcg gtcaataaaa taccggaaag agaataccag ccgaaaccgg aggccattgc
    13501 cagcgcggta ttgatgggaa gatcaagaat aaaggcgtta attaaaccac caattaatga
    13561 actggcaacc accaccacgg cgacaatcat tccccggcga ttaaggacaa tctgctttaa
    13621 ggtcatgcca ttattgcgca actgaatacc gacgaggaaa agcagcagaa ttaaggtgta
    13681 ttcactggct tcggtcgcgt gttgtaagaa agccagtcca ctcagaccaa tggcaaaacc
    13741 aatcactact acgccgcaca gttttagcga ctccagcgcc atcgcaatac gcgacgggag
    13801 tttttcttgc tgatggtggt tgctccatgg caggccgcgc tccagccaca tcagggcggc
    13861 aatattacac agtaaaataa cggtagtact gacggcagaa taatgcagaa ttgccaacaa
    13921 gttactggcg aggttatcga gaaacgccag actgataccc ataaaaaaga gaataaggta
    13981 aaccatccag cttaagagct gattaataac tcttaatgca gctttttggc gaagcggaat
    14041 gaggtaaccc acaatcaggg gaaccagaat gattaacagc ccagaaaaca tgaaaaccca
    14101 gtccttgcaa agaagattcg aaatgcgcga tgacacacta ctgaaagcgg aaggacgagt
    14161 aaagttgcaa ttaaaaggaa atgttatgca taaggatcgg tagggtattc attttcattt
    14221 aaagatattc ttgcgcttta attacaaacc gcaccggtgt tggtggcgtc aaaatcgccg
    14281 acccggtagc cgtaagggat aagtcgaggg caccgcgcag cggcgatttt gttcgccaga
    14341 gcccgggggt gcagggggag gaggcgattg tcctccccct gcgcgctcct tgcgccagtg
    14401 gcaatatgtg gcttagctca tgaaaggagc gcaacaagat gatgaatcaa catataacaa
    14461 catcttaaaa aaaggcctga cattgcgcca ggccttctgc gttaattaat tacgcttttc
    14521 cagtagagtc cggtaaatca gaccaccgat aataccgccg acaattggca ccacccagaa
    14581 gaaccacagt tgttctaatg cccagccgcc ctggaagata gcaaccgcag tactgcgcgc
    14641 cgggttaacg gaagtgttag tcaccggaat actgattaag tgaatcaggg ttaaggccag
    14701 accaatggca attggcgcaa aaccagcagg agcgaatttg tcagttgcgc cgtggatcac
    14761 cagcaggaaa cctgcactca ataccagttc aactaccagc gcggaaagca tggaataacc
    14821 gcctggtgaa tgctcgccat aaccgttaga agcaaaaccg ctggctgccg cgtcaaaacc
    14881 cgttttacca ctggcaatta aatacagcag cgccgccgca acaataccgc cgacaacctg
    14941 ggcaattacg tagccaacga cttcttttgc cgggaaacgt ccgccagccc ataaaccaat
    15001 agtgaccgcc gggttaaaat gaccaccaga aatatgacca acagcaaagg ccattgtcag
    15061 aacggtcaga ccgaacgcca acgccacgcc ggcaaaacca atgcctaatt ccgggaagcc
    15121 tgcggccagt acggcactac cacagccgcc aaaaacaagc cagaaagtac caaaacattc
    15181 agctgctaat tttctgaaca tatccacctc aattaaaaat tgaccctgtg aaaaatatgg
    15241 tcgttttata gggtcgtcgt aaaaaagtga cgacggaaat aatgcgcggc tattttaaaa
    15301 acgaaggcaa atcattcgcc agataaataa atccagtaaa tttgatttag ggcaacagcg
    15361 ggttgcccca tatagtcatt
//
LOCUS       DAHHTG010000102        15227 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.103, whole genome shotgun sequence.
ACCESSION   DAHHTG010000102 DAHHTG010000000
VERSION     DAHHTG010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15227)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15227)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15227
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.103"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            19..573
                     /locus_tag="NBI74_003314"
     CDS             19..573
                     /locus_tag="NBI74_003314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HCD1933598.1"
                     /translation="MNKKLMYIFAIFIVAAITCISQPKKTTLRDKAMANYAFDYLSSP
                     GSLPFTTAATELSAIHGHSTSQYRLGEFYLHGSDGKPLDYTQARYWYEQSAEQENPRA
                     QSKLGWIYLKGLGVKPDTRKAILWYKEAAEQGYAHAQYTLGLIYRNGSGINVNHYESQ
                     KWLKLAAKQHYKNAERLLAGLSAH"
     gene            713..1048
                     /locus_tag="NBI74_003315"
                     /pseudo
     CDS             713..1048
                     /locus_tag="NBI74_003315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706566.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
     gene            complement(1118..1600)
                     /locus_tag="NBI74_003316"
     CDS             complement(1118..1600)
                     /locus_tag="NBI74_003316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon-containing protein"
                     /protein_id="HCD1933599.1"
                     /translation="MNKVAQYYRELVASLSERLRNGERDIDALVEQARERVIKTGELT
                     RTEVDELTRAVRRDLEEFAMSYEESLKEESDSVFMRVIKESLWQELADITDKTQLEWR
                     EVFQDLNHHGVYHSGEVVGLGNLVCEKCHFHLPIYTPEVLTLCPKCGHDQFQRRPFEP
                     "
     gene            1835..4417
                     /gene="leuS"
                     /locus_tag="NBI74_003317"
     CDS             1835..4417
                     /gene="leuS"
                     /locus_tag="NBI74_003317"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008456064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HCD1933600.1"
                     /translation="MQEQYRPEEIESKVQLHWDEKRTFEVTEDESKEKYYCLSMLPYP
                     SGRLHMGHVRNYTIGDVIARYQRMLGKNVLQPIGWDAFGLPAEGAAVKNNTAPAPWTY
                     DNIAYMKNQLKMLGFGYDWSRELATCTPEYYRWEQKFFTELYKKGLVYKKTSAVNWCP
                     NDQTVLANEQVIDGCCWRCDTKVERKEIPQWFIKITAYADELLNDLDKLDHWPDTVKT
                     MQRNWIGRSEGVEITFNVNDYDNTLTVYTTRPDTFMGCTYLAVAAGHPLAQKAAENNP
                     ELAAFIDECRNTKVAEAEMATMEKKGVDTGFKAVHPLTGEEIPVWAANFVLMEYGTGA
                     VMAVPGHDQRDYEFASKYGLNIKPVILAADGSEPDLSQQALTEKGVLFNSGEFNGLDH
                     EAAFNAIADKLTAMGVGERKVNYRLRDWGVSRQRYWGAPIPMVTLEDGTVMPTPDDQL
                     PVILPEDVVMDGITSPIKADPEWAKTTVNGMPALRETDTFDTFMESSWYYARYTCPEY
                     KEGMLDSKAANYWLPVDIYIGGIEHAIMHLLYFRFFHKLMRDAGMVNSDEPAKQLLCQ
                     GMVLADAFYYVGENGERNWVSPVDAIVERDEKGRIVKAKDAAGHELVYTGMSKMSKSK
                     NNGIDPQVMVERYGADTVRLFMMFASPADMTLEWQESGVEGANRFLKRVWKLVYEHTA
                     KGDVAALNVDALTEDQKALRRDVHKTIAKVTDDIGRRQTFNTAIAAIMELMNKLAKAP
                     TDGEQDRALMQEALLAVVRMLNPFTPHICFTLWQELKGEGDIDNAPWPVADEKAMVED
                     STLVVVQVNGKVRAKITVPVDATEEQVRERAGQEHLVAKYLDGVTVRKVIYVPGKLLN
                     LVVG"
     gene            4432..5013
                     /gene="lptE"
                     /locus_tag="NBI74_003318"
     CDS             4432..5013
                     /gene="lptE"
                     /locus_tag="NBI74_003318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HCD1933601.1"
                     /translation="MRYLATLLLSLAVLITAGCGWHLRDTTQVPSTMKVMILDSGDPN
                     GPLSRAVRNQLRLNGVELLDKETTRKDVPSLRLGKVSIAKDTASVFRNGQTAEYQMIM
                     TVNATVLIPGRDIYPISAKVFRSFFDNPQMALAKDNEQDMIVKEMYDRAAEQLIRKLP
                     SIRAADIRSDEEQTSTTTDTPATPARVSTTLGN"
     gene            5013..6044
                     /gene="holA"
                     /locus_tag="NBI74_003319"
     CDS             5013..6044
                     /gene="holA"
                     /locus_tag="NBI74_003319"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HCD1933602.1"
                     /translation="MIRLYPEQLRAQLNEGLRAAYLLLGNDPLLLQESQDAVRQVAAA
                     QGFEEHHTFSIDPNTDWNAIFSLCQAMSLFASRQTLLLLLPENGPNAAINEQLLTLTG
                     LLHDDLLLIVRGNKLSKAQENAAWFTALANRSVQVTCQTPEQAQLPRWVAARAKQLNL
                     ELDDAANQVLCYCYEGNLLALAQALERLSLLWPDGKLTLPRVEQAVNDAAHFTPFHWV
                     DALLMGKSKRALHILQQLRLEGSEPVILLRTLQRELLLLVNLKRQSAHTPLRALFDKH
                     RVWQNRRGMMSEALNRLSQPQLRQAVQLLTRTELTLKQDYGQSVWAELEGLSLLLCHK
                     PLADVFIDG"
     gene            6046..6687
                     /gene="nadD"
                     /locus_tag="NBI74_003320"
     CDS             6046..6687
                     /gene="nadD"
                     /locus_tag="NBI74_003320"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HCD1933603.1"
                     /translation="MKSLQALFGGTFDPVHYGHLKPVETLANLIGLTRVTIIPNNVPP
                     HRPQPEANSMQRKHMLELAIADKPLFTLDERELKRNAPSYTAQTLKEWRQEQGPDVPL
                     AFIIGQDSLLTFPTWYEYETILDNAHLIVCRRPGYPLEMAQPQYQQWLEDHLTHNPED
                     LHLQPAGKIYLAETPWFNISATIIRERLQNGESCEDLLPEPVLTYINQQGLYR"
     gene            6711..7322
                     /locus_tag="NBI74_003321"
     CDS             6711..7322
                     /locus_tag="NBI74_003321"
                     /EC_number="3.1.3.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin/alpha-ribazole phosphatase"
                     /protein_id="HCD1933604.1"
                     /translation="MRLWLIRHGETQANVDGLYSGHAPTPLTARGIEQAQNLHTLLHD
                     VSFDLVLCSELERAQHTARLVLSDRQHPVHIIPELNEMFFGDWEMRHHRDLMQEDAEN
                     YSAWCNDWQQAIPTNGEGFQAFSQRVERFIARLSEYQHYQNILVVSHQGVLSLLIARL
                     IGMPAESMWHFRVDQGCWSAIDINQKFATLRVLNSRAIGVENA"
     gene            7582..7899
                     /gene="rsfS"
                     /locus_tag="NBI74_003322"
                     /gene_synonym="rsfA"
     CDS             7582..7899
                     /gene="rsfS"
                     /locus_tag="NBI74_003322"
                     /gene_synonym="rsfA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007373852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HCD1933605.1"
                     /translation="MQGKALQDFVIDKIDDLKGQDIIALDVQGKSSITDCMIICTGTS
                     SRHVMSIADHVVQESRAAGLLPLGVEGENSADWIVVDLGDVIVHVMQEESRRLYELEK
                     LWS"
     gene            7903..8370
                     /gene="rlmH"
                     /locus_tag="NBI74_003323"
     CDS             7903..8370
                     /gene="rlmH"
                     /locus_tag="NBI74_003323"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HCD1933606.1"
                     /translation="MKLQLVAVGTKMPDWVQTGFTEYLRRFPKDMPFELIEIPAGKRG
                     KNADIKRILDKEGEQMLAAAGKNRIVTLDIPGKPWDTPQLAAELERWKLDGRDVSLLI
                     GGPEGLSPACKAAAEQSWSLSALTLPHPLVRVLVAESLYRAWSITTNHPYHRE"
     gene            8401..10302
                     /gene="mrdA"
                     /locus_tag="NBI74_003324"
     CDS             8401..10302
                     /gene="mrdA"
                     /locus_tag="NBI74_003324"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-transpeptidase MrdA"
                     /protein_id="HCD1933607.1"
                     /translation="MKLQNSFRDYTAESALFVRRALVAFLGILLLTGVLIANLYNLQI
                     VRFTDYQTRSNENRIKLVPIAPSRGIIYDRNGIPLALNRTIYQIEMMPEKVDNVQQTL
                     DALRSVVDLTDDDIAAFRKERARSHRFTSIPVKTNLTEVQVARFAVNQYRFPGVEVKG
                     YKRRYYPYGSALTHVIGYVSKINDKDVERLNNDGKLANYAATHDIGKLGIERYYEDVL
                     HGQTGYEEVEVNNRGRVIRQLKEVPPQAGHDIYLTLDLKLQQYIETLLAGSRAAVVVT
                     DPRTGGVLALVSTPSYDPNLFVDGISSKDYSALLNDPNTPLVNRATQGVYPPASTVKP
                     YVAVSALSAGVITRNTTLFDPGWWQLPGSEKRYRDWKKWGHGRLNVTRSLEESADTFF
                     YQVAYDMGIDRLSEWMGKFGYGHYTGIDLAEERSGNMPTREWKQKRFKKPWYQGDTIP
                     VGIGQGYWTATPIQMSKALMILINDGIVKVPHLLMSTAEDGKQVPWVQPHEPPVGDIH
                     SGYWELAKDGMYGVANRPNGTAHKYFASAPYKIAAKSGTAQVFGLKANETYNAHKIAE
                     RLRDHKLMTAFAPYNNPQVAVAMILENGGAGPAVGTLMRQILDHIMLGDNNTDLPAEN
                     PAVAAAEDH"
     gene            10305..11417
                     /gene="mrdB"
                     /locus_tag="NBI74_003325"
                     /gene_synonym="rodA"
     CDS             10305..11417
                     /gene="mrdB"
                     /locus_tag="NBI74_003325"
                     /gene_synonym="rodA"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004885876.1"
                     /note="rod shape-determining protein RodA; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase MrdB"
                     /protein_id="HCD1933608.1"
                     /translation="MTDNPNKKTFWDKVHLDPTMLLILLALLVYSALVIWSASGQDIG
                     MMERKIGQIAMGLVIMVVMAQIPPRVYEGWAPYLYIICIILLVAVDAFGAISKGAQRW
                     LDLGIVRFQPSEIAKIAVPLMVARFINRDVCPPSLKNTAIALVLIFMPTLLVAAQPDL
                     GTSILVALSGLFVLFLSGLSWRLIGVAVVLVAAFIPILWFFLMHDYQRQRVMMLLDPE
                     SDPLGAGYHIIQSKIAIGSGGLRGKGWLHGTQSQLEFLPERHTDFIFAVLAEELGLVG
                     ILILLALYILLIMRGLWIAARAQTTFGRVMAGGLMLILFVYVFVNIGMVSGILPVVGV
                     PLPLVSYGGSALIVLMAGFGIVMSIHTHRKMLSKSV"
     gene            11428..12516
                     /gene="rlpA"
                     /locus_tag="NBI74_003326"
     CDS             11428..12516
                     /gene="rlpA"
                     /locus_tag="NBI74_003326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic peptidoglycan transglycosylase RlpA"
                     /protein_id="HCD1933609.1"
                     /translation="MRKQWLGICIAAGMLAACTSDDGQQQTVSVPQPAVCNGPIVEIS
                     GADPRFEPLNATANQDYQRDGKSYKIVQDPSRFSQAGLAAIYDAEPGSNLTASGEAFD
                     PTQLTAAHPTLPIPSYARITNLANGRMIVVRINDRGPYGNDRVISLSRAAADRLNTSN
                     NTKVRIDPIIVAQDGSLSGPGMACTTVAKQTYALPAPPDLSGGAGTSSVPVPQGDILP
                     VSNSTLKSEDPTGAPVTSSGFLGAPTTLAPGVLEGSEPTPAPQPVVTAPSTTPATSPA
                     MVTPQAASQSASGNFMVQVGAVSDQARAQQYQQQLGQKFGVPGRVTQNGAVWRIQLGP
                     FASKAEASTLQQRLQTEAQLQSFITTAQ"
     gene            12656..13867
                     /gene="dacA"
                     /locus_tag="NBI74_003327"
     CDS             12656..13867
                     /gene="dacA"
                     /locus_tag="NBI74_003327"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase DacA"
                     /protein_id="HCD1933610.1"
                     /translation="MNTIFSARIMKRLALTTALCTAFISAAHADDLNIKTMIPGVPQI
                     DAESYILIDYNSGKVLAEQNADVRRDPASLTKMMTSYVIGQAMKAGKFKETDLVTIGN
                     DAWATGNPVFKGSSLMFLKPGMQVPVSQLIRGINLQSGNDACVAMADFAAGSQDAFVG
                     LMNSYVNALGLKNTHFQTVHGLDADGQYSSARDMALIGQALIRDVPNEYSIYKEKEFT
                     FNGIRQLNRNGLLWDNSLNVDGIKTGHTDKAGYNLVASATEGQMRLISAVMGGRTFKG
                     REAESKKLLTWGFRFFETVNPLKVGKEFASEPVWFGDSDRASLGVDKDVYLTIPRGRM
                     KDLKASYVLNSSELHAPLQKNQVVGTINFQLDGKTIEQRPLVVLQEIPEGNFFGKIID
                     YIKLMFHHWFG"
     gene            13977..14240
                     /locus_tag="NBI74_003328"
     CDS             13977..14240
                     /locus_tag="NBI74_003328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbeD family protein"
                     /protein_id="HCD1933611.1"
                     /translation="MKTKLNELLEFPTPFTYKVMGQALPELVDQVVEVVQRHAPGDYT
                     PTVKPSSKGNYHSVSITINATHIEQVETLYEELGKIDIVRMVL"
     gene            14341..14982
                     /gene="lipB"
                     /locus_tag="NBI74_003329"
     CDS             14341..14982
                     /gene="lipB"
                     /locus_tag="NBI74_003329"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415163.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HCD1933612.1"
                     /translation="MYQDKILVRQLGLQPYEPISQAMHEFTDTRDESTLDEIWLVEHY
                     PVFTQGQAGKAEHILMPGDIPVIQSDRGGQVTYHGPGQQVMYVLLNLKRRKLGVRELV
                     TLLEQTVVNTLAELGIEAHPRADAPGVYVGEKKICSLGLRIRRGCSFHGLALNVNMDL
                     SPFLRINPCGYAGMEMAKISQWKPEATTNNIAPRLLENILALLNNPDFEYITA"
BASE COUNT         3787 a         3875 c         3935 g         3630 t
ORIGIN      
        1 aaatcgaagc attcacacat gaataaaaaa ttaatgtata tattcgcaat atttatagtt
       61 gcagcaataa cctgtattag ccaacccaag aaaacgacgt tgcgtgataa agccatggcg
      121 aattatgcct ttgattattt aagctcaccg ggcagtcttc cattcaccac ggcagccacg
      181 gagctttccg cgattcatgg tcactcaacg tcgcaatatc gccttggaga attttatctt
      241 catggtagcg acggtaaacc actggattat acacaggcga gatactggta tgagcaatca
      301 gcagaacagg aaaatccacg cgcgcaaagt aaactggggt ggatctacct caaaggtctg
      361 ggggtcaaac ccgacacccg taaagcaatt ctctggtata aggaagcagc tgaacaaggg
      421 tatgctcatg ctcaatatac tttgggtttg atctacagaa atggctcagg tattaatgtt
      481 aaccattatg aatctcaaaa atggttaaaa ctggccgcca aacaacatta caaaaatgcg
      541 gaaagattac ttgccgggct ttccgcacat taacgtcgac tgtcacccga acagatggag
      601 tattttccct atcaaaccag caaagataaa aatatagaaa ataattttaa tgatgcccag
      661 acagcctggc ccaccatcgt caacgggcat tggctgcggt tgcggctccg ataacgctgg
      721 cgaaggggtg gaccaggact ataagcaagc gatgtactgg tatctgaagg ctgctgctca
      781 ggaaagcgtc ggcgcttacg tcaacatcgg ttatatgtat aaacacggac aaggcgttga
      841 gaaagattat caggccgcct ttgaatagtt tacgatagcc gctgaatgca atgacgccac
      901 agcctggtat aacctggcca ttatgtatca ctacggagaa ggaagacctg tcgatctccg
      961 acaggctctc gacttgtatc gtaaagttca gtcatccgga accagagatg tcagtcaaga
     1021 aattcgtgag attgaagatt tactgtagag ataaaaacaa gggccgggga atttcattcg
     1081 gcccatattc tctttattgc tgtgcgaaat cagactatta cggctcaaac gggcgtctct
     1141 ggaactggtc atgaccacat ttagggcata gcgtcagcac ttccggtgtg tagatcggga
     1201 gatggaagtg acatttctcg cagaccagat ttcccagccc taccacttct ccgctgtgat
     1261 aaaccccatg atgattgagg tcctggaaaa cttcgcgcca ttcaagctgc gttttatcgg
     1321 tgatgtctgc cagctcctgc cacaagcttt ctttaatcac ccgcataaag acgctgtcag
     1381 actcttcttt caggctctct tcatagctca tggcgaactc ttccaggtca cgtctgacag
     1441 ctcgcgtcag ctcatcgacc tcggttcgcg ttaactcccc tgtttttatt acgcgctcgc
     1501 gcgcctgttc caccagtgcg tcgatatcac gttcgccatt gcgcaggcgt tcgctcagtg
     1561 acgcgaccag ttcacggtaa tattgagcaa ccttgttcat cgtttcatct cccgggtcgt
     1621 taactgacta taataataga cgttctttgt tccttgcttt gctaatacgc ccacaaactc
     1681 tgtaaattaa tgtatgaata atccgggcat catttcaacg tcgagtgcgc gaaaggctgt
     1741 tttgacgcgg gcgtttgggc tatgctatgc ggatctgaaa aaccacatca acgctacatt
     1801 tgtagccgta ttgaaaacag gaccactggc tgccatgcaa gagcaatacc gcccggaaga
     1861 gatagaatcc aaagtacagc ttcactggga tgagaagcgc acatttgaag taaccgaaga
     1921 cgagagcaaa gagaagtatt actgcctgtc tatgcttccc tatccttctg gtcgactaca
     1981 catgggccac gttcgcaact acaccatcgg tgacgtgatc gcccgctacc agcgcatgct
     2041 gggcaaaaac gttctgcagc caatcggctg ggacgcattt ggtctgcctg cggaaggcgc
     2101 ggcggtgaaa aacaacaccg cgccagcacc gtggacgtac gacaacatcg cgtatatgaa
     2161 aaaccagctc aaaatgctgg gctttggtta tgactggagc cgcgagctgg cgacctgtac
     2221 gccggaatac taccgttggg aacagaaatt cttcaccgag ctgtataaaa aaggcctggt
     2281 atataagaag acttctgcgg tcaactggtg cccgaacgac cagaccgtac tggcgaacga
     2341 acaagttatc gacggctgct gctggcgctg cgataccaaa gttgagcgta aagagatccc
     2401 gcagtggttt atcaaaatca cagcttacgc tgacgagctg ctcaacgatc tggataaact
     2461 ggatcactgg ccagacaccg ttaaaacaat gcagcgtaac tggatcggtc gttccgaagg
     2521 cgtggaaatc accttcaacg ttaacgacta tgacaacacg ctgaccgttt acactacccg
     2581 cccggacacc tttatgggtt gtacctacct ggcggtggct gcgggtcatc cgctggcaca
     2641 gaaagcggcg gaaaataatc ctgaactggc cgcctttatt gacgaatgcc gtaacaccaa
     2701 agttgccgaa gctgaaatgg cgacgatgga gaaaaaaggc gtcgatactg gctttaaagc
     2761 ggttcaccca ttaacgggcg aagaaattcc cgtttgggca gcaaacttcg tattgatgga
     2821 atacggcacg ggcgcagtta tggcggttcc gggtcacgac cagcgcgact acgagtttgc
     2881 ctctaaatac ggcctgaaca tcaaaccggt tatcctggca gctgacggct ctgagccaga
     2941 tctttctcag caagccctga ctgaaaaagg cgtgctgttc aactctggcg agtttaatgg
     3001 tcttgaccat gaagcggcct tcaacgccat cgccgataaa ctgactgcga tgggcgttgg
     3061 cgagcgtaaa gtgaactacc gcctgcgcga ctggggtgtt tctcgtcagc gttactgggg
     3121 cgcgccgatt ccgatggtga cgctggaaga cggtaccgta atgccgaccc cggacgacca
     3181 gctgccggtg atcctgccgg aagatgtggt gatggacggc attaccagcc cgattaaagc
     3241 agatccggag tgggcaaaaa ctaccgttaa cggtatgcca gcactgcgtg aaaccgacac
     3301 tttcgacacc tttatggagt cctcctggta ctatgcgcgc tacacttgcc cggagtacaa
     3361 agaaggtatg ctggattcca aagcggctaa ctattggctg ccggtggata tttacattgg
     3421 tggtatcgaa cacgccatta tgcacctgct ctacttccgc ttcttccaca aactgatgcg
     3481 tgatgcaggc atggtgaact ctgacgaacc agcgaaacag ttgctgtgtc agggtatggt
     3541 gctggcagat gccttctact atgttggtga aaacggcgaa cgtaactggg tttccccggt
     3601 tgatgctatc gttgaacgtg acgagaaagg ccgtatcgtg aaagcgaaag atgcggcagg
     3661 ccatgaactg gtttataccg gcatgagcaa aatgtccaag tcgaagaaca acggtatcga
     3721 cccgcaggtg atggttgaac gttacggcgc ggacaccgtt cgtctgttta tgatgtttgc
     3781 ttctccggct gatatgactc tcgaatggca ggaatccggc gtggaagggg ctaaccgctt
     3841 cctgaaacgt gtctggaaac tggtgtacga gcacacagca aaaggtgatg ttgcggcact
     3901 gaacgtcgat gcgctgactg aagatcagaa agcgctgcgt cgcgatgtgc ataaaactat
     3961 cgctaaagta accgatgata tcggccgtcg tcagaccttc aacaccgcaa ttgcggcgat
     4021 tatggagctg atgaacaaac tggcgaaagc accgaccgat ggcgagcagg atcgcgccct
     4081 gatgcaggaa gcgctgctgg cggtagtccg tatgcttaac ccgttcaccc cgcacatctg
     4141 cttcacgctg tggcaggaac tgaaaggcga aggcgatatc gacaacgcgc cgtggccggt
     4201 tgctgacgaa aaagcgatgg tggaagactc cacgctggtc gtggtgcagg ttaacggtaa
     4261 agtccgtgca aaaatcaccg ttccggtgga cgcaacggaa gaacaggttc gcgaacgtgc
     4321 tggccaggaa catctggtag caaaatatct tgatggcgtt actgtacgta aagtgattta
     4381 cgtaccaggt aaactcctca atctggtcgt tggctaagcg cgggaggaag cgtgcgatat
     4441 ctggcaacat tgttgttatc tctggcggtg ttaatcaccg ccgggtgtgg ctggcatctg
     4501 cgtgacacca cgcaggttcc ttccactatg aaagtcatga tcctggactc aggcgatccg
     4561 aacgggccat taagccgtgc ggtgcgtaac cagttacgtc tgaatggtgt cgagttgctt
     4621 gataaagaaa ccacgcgtaa ggacgttcca tccctgcgtt tgggtaaagt gagcatcgcg
     4681 aaagataccg catcggtatt ccgtaacggt caaacagcag agtatcagat gatcatgacg
     4741 gtgaatgcga ccgtgttgat ccccggccgt gacatctacc cgattagcgc caaggtcttc
     4801 cgttcgttct tcgataaccc gcaaatggcg ttagcgaaag ataacgaaca agacatgatc
     4861 gtaaaagaga tgtacgaccg tgctgccgaa cagctgattc gtaagctgcc aagcatccgt
     4921 gctgcagata ttcgttccga cgaagaacag acgtcgacca caacggatac tccggcaacg
     4981 cctgcacgcg tctccaccac gctgggtaac tgatgattcg gttgtacccg gaacaactcc
     5041 gcgcgcagct caatgaaggg ctgcgcgcgg cgtatctttt acttggtaac gatcctctgt
     5101 tattgcagga aagccaggac gctgttcgtc aggtcgctgc ggcgcaaggg ttcgaagaac
     5161 accacacttt ttccattgat cccaacaccg actggaatgc gattttttcg ttatgccagg
     5221 ccatgagcct gtttgccagt cgacaaacgc tattgctgtt gttacccgaa aacggaccga
     5281 atgcggcgat caatgaacaa cttctcacac tcaccggact tctgcatgac gatctactgt
     5341 tgatcgtccg cggtaataaa ttaagcaaag cgcaagaaaa tgccgcctgg tttactgctc
     5401 ttgcgaatcg cagcgtgcag gtgacctgtc agacaccgga gcaggctcag ctgccccgct
     5461 gggttgctgc gcgcgcaaaa cagctcaact tagaactgga tgacgctgca aatcaggtgc
     5521 tctgctactg ttatgaaggt aacctgctgg cgctggccca ggcactggag cgtttatcgc
     5581 tactctggcc ggacggcaaa ctgacattac cgcgcgttga acaggcggtg aatgatgccg
     5641 cgcatttcac cccttttcat tgggttgatg ctttgttgat gggaaaaagt aagcgcgcgt
     5701 tgcatattct tcagcaactg cgtctggaag gcagcgaacc ggttattttg ttgcgcacat
     5761 tacaacgtga actgttgtta ctggttaacc tgaaacgcca gtctgcccat acgccactgc
     5821 gtgcgttgtt tgataagcat cgggtatggc agaaccgccg gggcatgatg agcgaagcgt
     5881 taaatcgctt aagccagccg cagttacgtc aggccgtgca actcctgaca cgaacggaac
     5941 tcaccctcaa acaagattac ggtcagtcag tgtgggcaga gctggaaggg ttatctcttc
     6001 tgttgtgcca taaacccctg gcggacgtat ttatcgacgg ttgatatgaa atctttacag
     6061 gctctgtttg gcggcacctt tgatccggtg cactatggtc atcttaaacc cgtggaaacg
     6121 ctggcgaatt tgattggtct gacgcgggtc acaatcatcc ctaataatgt tcctccgcat
     6181 cgtccccagc cggaagcgaa cagcatgcag cgtaaacaca tgcttgaact ggcgattgcc
     6241 gacaagccat tatttactct tgatgaacgc gagctaaagc gcaatgcccc ctcttacact
     6301 gcgcaaacac tgaaagagtg gcggcaggaa caaggaccgg acgtgccgct ggcgtttatt
     6361 attggtcagg attcattgct gacctttccg acctggtacg aatacgaaac gatactcgac
     6421 aatgcacatt tgatcgtctg tcggcgtcca ggttacccac ttgaaatggc gcaaccgcaa
     6481 taccagcaat ggctggaaga tcatttgaca cataacccgg aagatcttca ccttcagcct
     6541 gccggtaaaa tttatctggc tgaaacgccg tggtttaaca tctcggcgac catcatccgc
     6601 gaacgtttgc aaaacggtga atcgtgtgag gatttattac cggaaccggt actgacttac
     6661 attaaccaac aaggcttgta tcgctgatac ctgtcgttac ggagggcatg atgcgactgt
     6721 ggttaattcg tcatggtgaa acgcaagcga atgtcgatgg tctgtacagc ggtcatgcgc
     6781 ccacacccct gaccgcacgc ggtattgagc aagcgcaaaa tctgcatacg ctgctacatg
     6841 atgtttcctt tgacctggtt ttatgcagtg aactggaacg ggcacagcat accgcgcgac
     6901 tggttcttag tgaccgccag caccccgttc atatcatccc tgaactcaat gaaatgtttt
     6961 ttggcgactg ggagatgcga catcatcgtg acctcatgca agaagatgcc gaaaactata
     7021 gcgcgtggtg caatgactgg cagcaagcta tccccacgaa cggtgaagga tttcaggcat
     7081 tttcgcaacg tgtggaacga tttatcgcaa ggcttagtga atatcagcac tatcagaata
     7141 ttttagtcgt cagccatcag ggcgtactga gtctgttaat tgctcgttta attggtatgc
     7201 ctgccgagtc tatgtggcat ttccgcgttg accaggggtg ttggagcgcc attgatatca
     7261 accaaaaatt cgcgacgcta cgcgtcctca atagccgtgc catcggggtc gagaatgcat
     7321 gacttttctg tttttttacg ggtaagccgc aacgaccatt gacagccccg ggcaggctga
     7381 tattctccgc agccagactt tttccgccag acacgacttt gtagaaattg ttttacaaaa
     7441 atggcgatgc aatctgcggc gcggggtggg atgatagccc actttcgaaa gccgattcgg
     7501 cgacaattgt cccgaaatcg cctctggttc aggtatactg acagaccatt tttatctatt
     7561 tgattcaccc agggggaaaa cttgcagggt aaagcactcc aggattttgt tatcgacaaa
     7621 attgatgacc tcaaaggtca ggacatcatc gccttagacg ttcagggcaa atccagcatc
     7681 accgactgca tgatcatctg tacgggtacg tccagccgcc atgttatgtc cattgctgat
     7741 cacgttgtgc aggagtctcg cgcagcgggc ctgttaccgc tcggcgtaga aggtgaaaac
     7801 agcgccgact ggattgtcgt ggatttgggc gatgtgattg tccatgtcat gcaggaagag
     7861 agccgtcgcc tgtatgaact ggaaaaactc tggagttaat gcgtgaagct gcaacttgtc
     7921 gccgtgggaa cgaaaatgcc ggactgggta caaaccggtt ttaccgagta cctgcgtcgt
     7981 tttccgaaag atatgccctt cgagctgatt gaaattccgg ccggaaaacg cggcaagaat
     8041 gcggacatca agcgcatact cgacaaagag ggtgagcaga tgttggcggc cgcaggcaaa
     8101 aaccgcattg tcaccctcga tattccaggc aagccctggg atacgccgca gttagccgct
     8161 gagctggaac gctggaagct ggatggtcgc gacgtcagtc tactgattgg cgggcctgaa
     8221 gggttgtcgc ctgcctgtaa agcggcggcg gagcagagct ggtcgctgtc ggcacttacc
     8281 cttccccatc cgctggttcg cgtgctggtc gcagagagtc tgtaccgggc gtggagcatc
     8341 accaccaacc atccttatca ccgtgagtga taagtgagct ttgagtagaa aacgcagcgg
     8401 atgaaactac agaactcttt tcgcgactat acggcagagt ccgcgctgtt tgtgcgccgg
     8461 gcgctggtcg cctttttggg gattttgctg ctgaccggcg tgcttatcgc caacctgtat
     8521 aatctgcaaa ttgttcgctt taccgactac cagacccgct ctaatgaaaa ccgcattaag
     8581 ctggtgccta tcgcacccag ccgcggcatt atctacgacc gtaacggtat ccctctggct
     8641 ctcaaccgca ctatctacca gatagaaatg atgccggaga aagtcgataa cgtgcagcaa
     8701 acgctggacg ctttgcgcag cgtggttgat ctgaccgatg atgatattgc tgcattccga
     8761 aaagagcgcg cacgttcaca ccgtttcacc tctattccgg tgaaaactaa cctgaccgaa
     8821 gtacaagtag ctcgctttgc cgtcaaccag taccgttttc cgggtgttga agttaaaggc
     8881 tataaacgtc gttactatcc ttacggttcg gcgttgaccc acgtcatcgg ctatgtgtcg
     8941 aaaatcaacg ataaagacgt cgaacgcctg aataatgacg gcaaactggc caactatgcg
     9001 gcaacgcatg atatcggtaa gctgggcatt gagcgttact atgaagacgt gctgcacggt
     9061 cagaccggtt atgaagaggt tgaagttaac aaccgtgggc gtgttatccg ccagttaaaa
     9121 gaagtaccac cgcaggccgg acacgatatt tacctgacgc tggatctcaa actccagcaa
     9181 tatattgaaa cgctgctggc gggtagccgc gcagctgtgg tagtcaccga tccgcgtacc
     9241 ggtggggtgt tggcgctggt ttccacgcct agttatgacc caaacctgtt tgttgacggt
     9301 atctccagca aagattattc cgccttgctg aacgacccga atacaccgct ggtgaaccgc
     9361 gccacacagg gggtttatcc tcccgcgtct acagttaaac cctatgtggc ggtttcggca
     9421 ttgagcgccg gagtgatcac gcgcaatacg acgttgtttg accccggctg gtggcaactt
     9481 ccaggttcgg aaaaacgtta tcgtgactgg aaaaaatggg gccacgggcg tctgaatgtc
     9541 acaagatcgc tggaagaatc tgcggatacc ttcttctatc aggtggccta cgatatgggg
     9601 atcgatcgcc tctccgaatg gatgggtaaa ttcggttatg gtcattacac cggtatcgac
     9661 ctggcggaag aacgttccgg caacatgcct acccgcgaat ggaaacagaa acgctttaaa
     9721 aaaccgtggt atcagggtga caccattccg gttggtatcg gtcagggtta ctggacagcg
     9781 accccaatcc agatgagtaa ggcactgatg atcctgatta atgacggtat cgtgaaggtt
     9841 ccgcatttgc tgatgagcac cgccgaagac ggcaaacagg tgccatgggt acaaccgcat
     9901 gaaccgcccg tcggcgatat tcattccggt tactgggagc tggcgaaaga cggtatgtac
     9961 ggtgttgcta accgccctaa cggtacggcg cataaatatt ttgccagcgc accgtacaaa
    10021 attgcggcga aatccggtac cgctcaggtc ttcggtctga aagcgaacga aacctataat
    10081 gcgcacaaaa ttgccgagcg tttacgtgac cacaaactga tgaccgcctt tgcgccatac
    10141 aacaatccgc aagtggctgt cgccatgatt ctggagaacg gtggcgcggg tccggcggtt
    10201 ggtacgctga tgcgccagat cctcgaccac attatgctgg gtgataacaa caccgatctg
    10261 cctgcggaaa atccagcggt tgccgcagcg gaggaccatt aatcatgacg gataatccga
    10321 ataaaaaaac attctgggat aaagtccatc tcgatcccac aatgctgctg atcttactgg
    10381 cattgctggt ttacagcgcc ctggttatct ggagcgccag cggtcaggat attggcatga
    10441 tggagcgtaa aatcggccag attgcgatgg gtctggtcat catggtggtg atggcgcaaa
    10501 ttcctccacg cgtttatgaa ggctgggccc cctatctcta tatcatctgt attattttgc
    10561 tggtggcggt agatgctttc ggtgccatct ctaaaggcgc tcaacgctgg ctggacctcg
    10621 gtattgttcg ttttcaaccg tcggaaattg ccaagatagc cgtaccgttg atggttgcac
    10681 gctttatcaa ccgcgacgtt tgcccacctt cattgaagaa tacagctatc gcgctggtgc
    10741 tgatatttat gcccacgctg ctggtggctg cacagcctga cctgggaaca tcaatcctcg
    10801 ttgcgctttc cggcctgttt gtactgttcc tctctggcct tagctggcgt ctgattggcg
    10861 tcgcagtagt gctggtagcg gcgttcattc cgattctgtg gttcttcctg atgcatgatt
    10921 accagcgcca gcgcgtaatg atgctcctgg acccggaatc agacccactc ggcgcaggct
    10981 atcacattat tcagtctaaa attgctattg gctccggcgg attacgcggc aaaggctggc
    11041 tgcacggcac tcagtcacag cttgaatttc tccccgaacg ccataccgac tttatcttcg
    11101 cggtactggc ggaagagctg ggattagtgg gcattttgat tctgctcgct ctctacattc
    11161 tgctgatcat gcgcgggctg tggatagccg ccagagcgca aaccaccttt ggtcgcgtca
    11221 tggctggcgg cttaatgctg atattattcg tttatgtctt cgtaaatatt ggtatggtaa
    11281 gcggtattct gccggttgta ggggttccgc tcccactggt cagttatgga ggatcggcgc
    11341 taattgtgct gatggctggg ttcgggattg taatgtcaat ccacacccac aggaaaatgt
    11401 tgtcgaaaag cgtgtaagag gtgcgcaatg cgtaagcagt ggctcgggat ctgcatcgcg
    11461 gcaggaatgc tcgcggcatg tacaagcgat gatggtcagc aacagacggt aagtgtaccg
    11521 cagcctgcgg tatgtaacgg ccccatagtt gaaattagcg gggcagaccc gcgtttcgaa
    11581 ccactgaacg cgacggcaaa tcaggattac cagcgcgacg gtaaaagcta caaaatcgtg
    11641 caggatccgt ctcgatttag ccaggcggga ctggcggcaa tctatgatgc cgaacccggc
    11701 agtaacctga cggcctctgg cgaagctttc gatccgacac agttgacggc ggcccatcca
    11761 acgcttccga tccccagcta cgccagaatc actaacctgg ctaacgggcg aatgatcgtg
    11821 gtgcgcatta atgatcgcgg cccttatggc aacgaccgcg tgatttcgct ttctcgcgcg
    11881 gcagctgacc gtcttaacac gtcaaacaac accaaagttc gtatcgatcc gattattgtc
    11941 gcccaggatg gttcgctttc tggtcctggt atggcttgta ccacagtcgc caaacagact
    12001 tacgccctgc cagcacctcc cgatttaagc ggtggcgcgg gaacaagttc agtgcctgtc
    12061 ccgcagggtg acattcttcc ggtcagtaat tcgacgctaa aaagcgaaga tccgaccggc
    12121 gcgccggtaa ccagcagcgg tttcctcggc gcaccaacga ccttagcgcc tggtgtactg
    12181 gaaggcagcg aaccgacgcc tgcgccacag cccgttgtta cagctccgtc gacaacgcct
    12241 gcaacctcac ctgcaatggt gacaccgcaa gccgcctcgc aaagcgccag cggcaacttt
    12301 atggtgcaag tcggggccgt tagcgatcag gctcgtgcgc aacagtacca acagcaactg
    12361 ggacagaagt tcggcgtccc cggtcgcgta actcaaaatg gcgcggtctg gcggatccag
    12421 cttggcccat tcgccagcaa agccgaagcc agtaccttgc agcaacgttt gcaaaccgaa
    12481 gcccaattac agtcatttat taccaccgcg cagtagcgta aagcaggcat ctgatgtgtc
    12541 aatttctgta aatgacgtta acaaagtcac gcggaaagtc agatgcctgc cggtagtgca
    12601 tttgctatag tagggcactt tttttaattc catcacggat gtcgtagttc agaccatgaa
    12661 taccattttt tccgctcgta tcatgaagcg cctggcgctc accacggctc tttgcacagc
    12721 ctttatctct gctgcacatg ccgatgacct gaatatcaaa accatgatcc cgggtgtacc
    12781 gcagatcgac gcggagtcct acatcctgat tgactataac tccggcaaag tactcgccga
    12841 acagaacgct gatgtccgcc gcgatcctgc cagcctgacc aaaatgatga ccagttacgt
    12901 tatcggccag gcaatgaaag ccggtaaatt taaagaaact gatttagtca ctatcggcaa
    12961 cgacgcatgg gccaccggta acccggtgtt taaaggttct tcgctgatgt tcctcaaacc
    13021 gggcatgcag gttccggttt ctcagctgat ccgcggtatt aacctgcaat cgggtaacga
    13081 tgcttgtgtc gccatggccg attttgccgc tggtagccag gacgcttttg ttggcttgat
    13141 gaacagctac gttaacgccc tgggcctgaa aaatacccac ttccagacgg tacacggtct
    13201 ggatgctgat ggtcagtaca gctccgcgcg agatatggcg ctgatcggcc aggcgttgat
    13261 ccgtgacgta ccgaatgaat actcgatcta taaagaaaaa gaatttacgt ttaacggtat
    13321 tcgccagctg aaccgtaacg gcctgttatg ggataacagc ctgaatgtcg acggcatcaa
    13381 aaccggacac actgacaaag caggttacaa ccttgttgct tctgcgactg aaggccagat
    13441 gcgcttgatc tctgcggtga tgggcggacg tacttttaaa ggccgtgaag ccgaaagtaa
    13501 aaaattgcta acctggggct tccgtttctt cgaaaccgtt aatcctctga aagtaggtaa
    13561 agagttcgcc tctgaaccgg tttggtttgg tgattctgat cgcgcttcgt taggggttga
    13621 taaagacgtg tacctgacca ttccgcgtgg tcgcatgaaa gatctgaaag ctagctatgt
    13681 gctgaacagc agtgaattgc atgcgccgct gcaaaagaat caggtcgtcg gaactatcaa
    13741 cttccagctt gacggcaaaa cgatcgaaca acgcccgctg gttgtgctgc aagaaatccc
    13801 ggaaggtaac ttcttcggca aaatcattga ttacattaaa ttaatgttcc atcactggtt
    13861 tggttaaaaa ttgaacactt gaaagtgtaa tttccgtccc catatactaa gcatcagtaa
    13921 aaaactcccg ccttctggcg ggagttgcta tttaattacg ttacgccgga gctgacatga
    13981 aaaccaaact taacgaactg cttgaattcc ctactccttt tacttacaaa gttatggggc
    14041 aggcgttacc tgagctggtt gatcaggtgg ttgaagtggt acagcgccat gcgccaggtg
    14101 actacacccc aacggtaaaa ccaagcagca aaggcaacta ccactcggta tctatcacta
    14161 tcaacgccac tcatatcgag caggttgaaa cactgtatga agaactgggc aaaatcgata
    14221 ttgtccgcat ggttctgtaa ctcgcttctc cgttacccgg ctcctggtcg ggtaactccc
    14281 ctctcctcgc tgtgatatac ttttccccca cttttactca ttctccacgg agatgccgtt
    14341 ttgtatcagg ataaaattct tgtccgccag ctcggtcttc agccttacga gccaatctcc
    14401 caggctatgc atgaattcac cgatacccgc gatgaaagta cccttgatga aatctggctg
    14461 gtcgagcact atccggtatt cacccaaggg caggcaggaa aggcggagca cattttaatg
    14521 ccgggtgata ttccggtgat ccagagcgat cgcggtgggc aggtgactta tcacgggccg
    14581 gggcaacagg tgatgtatgt gttgcttaac ctgaaacgcc gtaaactcgg tgtgcgtgaa
    14641 ctggtgacct tgcttgagca aacagtggtg aataccctgg ctgaactggg tatagaagcg
    14701 catcctcggg ctgacgcgcc tggtgtctat gtcggggaaa agaaaatttg ctcactgggt
    14761 ttacgtattc gacgcggttg ttcattccac ggtctggcat taaacgtcaa tatggatctt
    14821 tcaccatttt tacgtattaa tccttgtggg tatgcaggaa tggaaatggc taaaatatca
    14881 caatggaaac ccgaagcgac gactaataat attgcaccac gtttactgga aaatatttta
    14941 gcgctactaa acaatcctga cttcgaatat attaccgctt aattccatac atcaatggcc
    15001 caatttacac tgggtcatta ctcataatac ctgctaatgc tttacatctc cttcgcgttt
    15061 gtatattttc tttatcactt gcagaaatgc gatgctacgc cttgctccgt agcccaccta
    15121 attgattaac tcattgaaat aattgaccat ggtgagcaaa agcgttccgt cccaattaga
    15181 cataagatta ttgtaatttt tttaaatatt attttttcat gaataaa
//
LOCUS       DAHHTG010000103        15182 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.104, whole genome shotgun sequence.
ACCESSION   DAHHTG010000103 DAHHTG010000000
VERSION     DAHHTG010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15182)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15182)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15182
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.104"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            12..1286
                     /gene="uraA"
                     /locus_tag="NBI74_003330"
     CDS             12..1286
                     /gene="uraA"
                     /locus_tag="NBI74_003330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HCD1933613.1"
                     /translation="MGVSERPPLLQTIPLSLQHLFAMFGATVLVPVLFHINPATVLLF
                     NGIGTLLYLFICKGKIPAYLGSSFAFISPVLLLLPLGYEVALGGFIMCGVLFCLVSFI
                     VKKAGTGWLDVLFPPAAMGAIVAVIGLELAGVAAGMAGLLPAEGQTPDSKTIIISITT
                     LAVTVLGSVLFRGFLAIIPILIGVLVGYALSFAMGIVDTTPIINAHWFALPTLYTPRF
                     EWFAILTILPAALVVIAEHVGHLVVTANIVKKDLLRDPGLHRSMFANGLSTVISGFFG
                     STPNTTYGENIGVMAITRVYSTWVIGGAAIFAILLSCVGKLAAAIQMIPLPVMGGVSL
                     LLYGVIGASGIRVLIESKVDYNKAQNLILTSVILIIGVSGAKVNIGAAELKGMALATI
                     VGIGLSLIFKLISMLRPEEVVLDAEDADITDK"
     gene            1336..2082
                     /gene="hda"
                     /locus_tag="NBI74_003331"
     CDS             1336..2082
                     /gene="hda"
                     /locus_tag="NBI74_003331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA inactivator Hda"
                     /protein_id="HCD1933614.1"
                     /translation="MVNFSRFCEILVEVSLNTPAQLSLPLYLPDDETFASFWPGDNSS
                     LLAALQNVLRQEHSGYIYLWAREGAGRSHLLHAACAELSQRGDAVGYVPLDKRTWFVP
                     EVLDGMEHLSLVCIDNIECIAGDELWEMAIFDLYNRILESGKTRLLITGDRPPRQLNL
                     GLPDLASRLDWGQIYKLQPLSDEDKLQALQLRARLRGFELPEDVGRFLLKRLDREMRT
                     LFMTLDQLDRASITAQRKLTIPFVKEILKL"
     gene            complement(2220..2579)
                     /gene="arsC"
                     /locus_tag="NBI74_003332"
     CDS             complement(2220..2579)
                     /gene="arsC"
                     /locus_tag="NBI74_003332"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311384.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HCD1933615.1"
                     /translation="MTKQVKIYHNPRCSKSRETLNLLKENGVEPEVVLYLETPADAAT
                     LRDLLKMLGMNSARELMRQKEDLYKELNLADSSLSEEALIQAMVDNPKLMERPIVVAN
                     GKARIGRPPEQVLEIVG"
     gene            complement(2600..4063)
                     /gene="bepA"
                     /locus_tag="NBI74_003333"
     CDS             complement(2600..4063)
                     /gene="bepA"
                     /locus_tag="NBI74_003333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-barrel assembly-enhancing protease"
                     /protein_id="HCD1933616.1"
                     /translation="MFRQLKKNLVATLIAAMTIGQVAPAFADSADTLPDMGTSAGSTL
                     SIGQEMQMGDYYVRQLRGSAPLINDPLLTQYINSLGMRLVSHANSVKTPFHFFLINND
                     EINAFAFFGGNVVLHSALFRYSDNESQLASVMAHEISHVTQRHLARAMEDQQRSAPLT
                     WVGALGSILLAMASPQAGMAALTGTLAGTRQGMISFTQQNEQEADRIGIQVLQRSGFD
                     PQAMPTFLEKLLDQARYSSRPPEILLTHPLPESRLADARNRANQMRPMVVQSSEDFYL
                     AKARTLGMYNSGRNQLTSDLLDEWAKGNVRQQRAAQYGRALQAMEANKYDEARKTLQP
                     LLAAEPGNAWYLDLATDIDLGQNKANDAINRLKNARDLRTNPVLQLNLANAYLQGGQP
                     QEAANILNRYTFNNKDDSNGWDLLAQAEAALNNRDQELAARAEGYALAGRLDQAISLL
                     SSASSQVKLGSLQQARYDARIDQLRQLQERFKPYTKM"
     gene            4276..5337
                     /locus_tag="NBI74_003334"
     CDS             4276..5337
                     /locus_tag="NBI74_003334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HCD1933617.1"
                     /translation="MLEMLMQWYRRRFSDPEAIALLVILVAGFGIIFFFSGLLAPLLV
                     AIVLAYLLEWPTVRLQSIGCSRRWATSIVLVVFVGILLLMAFVVLPIAWQQGIYLIRD
                     MPGMLNKLSDFAATLPRRYPALMDAGIIDAMAENMRSRMLTMGDSVVKISLASLVGLL
                     TIAVYLVLVPLMVFFLLKDKEQMLNAVRRVLPRNRGLAGQVWKEMNQQITNYIRGKVL
                     EMIVVGIATWLGFLLFGLNYSLLLAVLVGFSVLIPYIGAFVVTIPVVGVALFQFGAGT
                     EFWSCFAVYLIIQALDGNLLVPVLFSEAVNLHPLVIILSVVIFGGLWGFWGVFFAIPL
                     ATLIKAVIHAWPDGQIAQD"
     gene            complement(5435..5905)
                     /gene="bcp"
                     /locus_tag="NBI74_003335"
     CDS             complement(5435..5905)
                     /gene="bcp"
                     /locus_tag="NBI74_003335"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HCD1933618.1"
                     /translation="MNPLKAGDIAPKFSLPDQDGEQVNLTDFQGQRVLVYFYPKAMTP
                     GCTVQACGLRDNMDELKKAGVDVLGISTDKPEKLSRFAEKELLNFTLLSDEDHQVCEQ
                     FGVWGEKSFMGKTYDGIHRISFLIDADGKIEHVFDDFKTSNHHDVVLNWLKEHA"
     gene            complement(5905..6477)
                     /locus_tag="NBI74_003336"
     CDS             complement(5905..6477)
                     /locus_tag="NBI74_003336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     repressor"
                     /protein_id="HCD1933619.1"
                     /translation="MTLSSQHYLVITALGADRPGIVNTITRHVSSCGCNIEDSRLAML
                     GEEFTFIMLLSGSWNAITLIESTLPLKGAELDLLIVMKRTTARPRPPMPASVWVQVDV
                     ADSPHLIERFTALFDAHHMNIAELVSRTQPAENERAAQLHIQITAHSPASADAANIEQ
                     AFKALCTELNAQGSINVVNYSQHDEQDGVK"
     gene            6623..7501
                     /gene="dapA"
                     /locus_tag="NBI74_003337"
     CDS             6623..7501
                     /gene="dapA"
                     /locus_tag="NBI74_003337"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001311023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HCD1933620.1"
                     /translation="MFTGSIVAIVTPMDEKGNVCRASLKKLIDYHVASGTSAIVSVGT
                     TGESATLNHDEHADVVMMTLELADGRIPVIAGTGANATAEAISLTQRFNDSGIVGCLT
                     VTPYYNRPSQEGLYQHFKAIAEHTDLPQILYNVPSRTGCDLLPETVGRLAKVKNIIGI
                     KEATGNLTRVNQIKELVSDDFVLLSGDDASALDFMQLGGHGVISVTANVAARDMAQMC
                     KLAAEGHFAEARVINQRLMPLHNKLFVEPNPIPVKWACKELGLVATDTLRLPMTPITD
                     SGRETVRAALKHAGLL"
     gene            7518..8552
                     /gene="bamC"
                     /locus_tag="NBI74_003338"
     CDS             7518..8552
                     /gene="bamC"
                     /locus_tag="NBI74_003338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamC"
                     /protein_id="HCD1933621.1"
                     /translation="MAYSVQKSRLAKVAGVSLVLLLAACSSDSRYKRQVSGDEAYLEA
                     APLAELHAPAGMILPVTSGDYAIPVTNGSGAVGKALDIRPPAQPLALVSGARTQFTGD
                     TASLLVENGRGNTLWPQVVSVLQAKNYTITQRDDAGQTLTTDWVQWNRLDEDEQYRGR
                     YQISVKPQGYQQAVTVKLLNLEQAGKPVADAASMQRYSTEMMNVISAGLDKSATDAAN
                     AAQNRASTTMDVQSAADDTGLPMLVVRGPFNVVWQRLPAALEKVGMKVTDSTRSQGNM
                     AVTYKPLSDSEWHELGASDPGLASGDYKLQVGDLDNRSSLQFIDPKGHTLTQSQNDAL
                     VAVFQAAFSK"
     gene            8765..9478
                     /gene="purC"
                     /locus_tag="NBI74_003339"
     CDS             8765..9478
                     /gene="purC"
                     /locus_tag="NBI74_003339"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HCD1933622.1"
                     /translation="MQKQAELYRGKAKTVYSTENPDLLVLEFRNDTSAGDGARIEQFD
                     RKGMVNNKFNYFIMSKLAEAGIPTQMERLLSDTECLVKKLDMVPVECVVRNRAAGSLV
                     KRLGIEEGIELNPPLFDLFLKNDAMHDPMVNESYCETFGWVSKENLARMKELTYKAND
                     VLKKLFDDAGLILVDFKLEFGLYKGEVVLGDEFSPDGSRLWDKETLEKMDKDRFRQSL
                     GGLIEAYEAVARRLGVQLD"
     gene            9646..10509
                     /locus_tag="NBI74_003340"
     CDS             9646..10509
                     /locus_tag="NBI74_003340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HCD1933623.1"
                     /translation="MRWQGRRESDNVEDRRNSSGGPSMGGPGFRLPSGKGGLILLIVV
                     LVAGYYGVDLTGLMTGQPVSQQQSTRSISPNEDEAAKFTSVILATTEDTWGQQFEKMG
                     KTYQQPKLVMYRGMTRTGCGAGQSIMGPFYCPADGTVYIDLSFYDDMKDKLGADGDFA
                     QGYVIAHEVGHHVQKLLGIEPKVRQLQQNATQAEVNRLSVRMELQADCFAGVWGHSMQ
                     QQGVLETGDLEEALNAAQAIGDDRLQQQSQGRVVPDSFTHGTSQQRYSWFKRGFDSGD
                     PAQCNTFGKSI"
     gene            10524..12539
                     /gene="tmcA"
                     /locus_tag="NBI74_003341"
     CDS             10524..12539
                     /gene="tmcA"
                     /locus_tag="NBI74_003341"
                     /EC_number="2.3.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cytosine(34) acetyltransferase TmcA"
                     /protein_id="HCD1933624.1"
                     /translation="MAELTALHTLTAQMKREGIRRLLVLSGEERWCFDHALKLRDALP
                     GDWLWISPQPDAENHCSPSALQTLLGREFRHAVFDARHGFDAAAFAALSGTLKAGSWL
                     VLLLPVWDEWENQPDADSLRWSDCPDPIATPHFVQHFKRVLTANNDAILWRRNQPFSL
                     AHFTPRTDWHPATGAPQPEQQQLLQQLLTMPPGVAAVTAARGRGKSALAGQLISRIAG
                     SAIVTAPAKAATDVLAQFAGEKFRFIAPDALLASDEQADWLVVDEAAAIPAPLLYQLV
                     SRFPRTLLTTTVQGYEGTGRGFLLKFCARFPHLHRFELQQPIRWAQGCPLEKMVSEAL
                     VFDDENFTHTPQGNIVISAFEQTLWRSEPETPLKVYQLLSGAHYRTSPLDLRRMMDAP
                     GQHFLQAAGGNEIAGALWLVDEGGLSQELSQAVWAGFRRPRGNLVAQSLAAHGSNPLA
                     ATLRGRRVSRIAVHPTRQREGTGRQLIAGALQYIHDLDYLSVSFGYTEELWRFWQRCG
                     FVLVRMGNHREASSGCYTAMALLPMSDAGKQLAEREHYRLRRDAQALAQWNGEMLPVD
                     PLNDAVLSDDDWLELAGFAFTHRPLLTSLGCLLRLLQTSELALPALRGRLQKNASDAQ
                     LCTTLKLSGRKLLLVRQREEAAQALFALDDVRTERLRDRITQWQFFH"
     gene            12613..13311
                     /gene="ypfH"
                     /locus_tag="NBI74_003342"
     CDS             12613..13311
                     /gene="ypfH"
                     /locus_tag="NBI74_003342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HCD1933625.1"
                     /translation="MKHDHFVVQSPDKPAQQLLLLFHGVGDNPVAMGEIGSWFAPLFP
                     DALVVSVGGAEPSGNPAGRQWFSVQGITEDNRQARVNAIMPTFIETVRYWQKQSGVGA
                     NATALIGFSQGAIMALESIKAEPGLASRVIAFNGRYASLPETASTATTIHLIHGGEDP
                     VIDLAHAVAAQEALISAGGDVTLDIVEDLGHAIDNRSMQFALDHLRYTIPKHYFDEAL
                     SGGKPGDDDVIEMM"
     gene            complement(13392..13592)
                     /locus_tag="NBI74_003343"
     CDS             complement(13392..13592)
                     /locus_tag="NBI74_003343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpfN family protein"
                     /protein_id="HCD1933626.1"
                     /translation="MDWLAKYWWILVIVFLVGVLLNVIKDLKRVDHKKFLANKPELPP
                     HRDFNDKWDDDDDWPKKDQPKK"
     gene            complement(13620..14747)
                     /gene="dapE"
                     /locus_tag="NBI74_003344"
     CDS             complement(13620..14747)
                     /gene="dapE"
                     /locus_tag="NBI74_003344"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020078199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HCD1933627.1"
                     /translation="MSCPVIELTQQLIRRPSLSPDDAGCQALLIERLQAIGFTVERMD
                     FADTQNFWAWRGQGETLAFAGHTDVVPPGDADRWINPPFEPTIRDGMLFGRGAADMKG
                     SLAAMVVAAERFVAQHPNHTGRLAFLITSDEEASAHNGTVKVVEALMARNERLDYCLV
                     GEPSSIEVVGDVVKNGRRGSLTCNLTIHGVQGHVAYPHLADNPVHRAAPFINELVAIE
                     WDQGNDFFPATSMQIANIQAGTGSNNVIPGELFVQFNFRFSTELTDEMIKAQVLALLE
                     KHQLRYTVDWWLSGQPFLTARGKLVDAVVNAVEHYNEIKPQLLTTGGTSDGRFIARMG
                     AQVVELGPVNATIHKINECVNAADLQLLARMYQRIMEQLVA"
     gene            complement(14751..15107)
                     /locus_tag="NBI74_003345"
     CDS             complement(14751..15107)
                     /locus_tag="NBI74_003345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC family reductase"
                     /protein_id="HCD1933628.1"
                     /translation="MVTLYGIKNCDTIKKARRWLEANNIDYRFHDYRVDGLDSELLNG
                     FINELGWEALLNTRGTTWRKLDETTRNKITDAASAATLMTEMPAIIKRPLLCAPGKPM
                     LLGFSESSYQQFFHEV"
BASE COUNT         3388 a         3801 c         4156 g         3837 t
ORIGIN      
        1 cgcgccgtgc tatcggggtg agtgaaagac cgccactttt acagacaatc ccgcttagtt
       61 tgcaacattt gttcgccatg tttggtgcaa ccgtcctggt gcccgtctta tttcatatta
      121 acccggcgac tgtactgtta tttaacggta taggaacact gctgtatctc ttcatctgta
      181 aagggaaaat tccggcttat cttggttcca gctttgcctt tatttcaccg gtattgttac
      241 tgttgccgtt ggggtatgaa gtcgcgctgg gcggctttat tatgtgcggc gtgctgttct
      301 gcctggtttc ttttatcgtg aagaaagcgg ggaccggctg gctggacgtg ctgtttccac
      361 ctgcggcaat gggcgcaatc gttgccgtca tcggtctgga gctggcgggc gtagctgccg
      421 gtatggcggg tttactcccg gctgaagggc aaacgccaga ctccaaaacc atcatcatct
      481 ctattaccac cctggcggtc acggttttag gttccgtgct gtttcgtggt ttcctggcaa
      541 ttatcccgat tttaattggc gtgctggtgg ggtacgcgct ctctttcgca atgggaattg
      601 tcgataccac gccgattatt aatgctcact ggtttgcgct gccgaccctc tatacgccgc
      661 gcttcgagtg gtttgccatt ctgactattc tgccagcggc gttagtggtt attgccgaac
      721 acgtagggca cctggtagta acggctaata tcgtcaaaaa agatctgctg cgcgatccag
      781 gtctgcaccg ttcgatgttt gctaacggct tgtcgaccgt gatctccggc ttctttggct
      841 ccacgccaaa caccacttac ggagaaaaca ttggcgtgat ggcgatcacc cgtgtttaca
      901 gtacctgggt tatcggcggt gcggcgattt tcgctatcct gctttcctgc gtcggtaaac
      961 tggctgctgc tatccagatg attccattgc cggtgatggg cggcgtttcg ctgctgcttt
     1021 acggtgtcat cggtgcttcc ggtattcgtg tgttgatcga atcgaaagtg gattacaaca
     1081 aagcacagaa cctgatcctg acttccgtga ttttgatcat cggcgtcagt ggcgcgaagg
     1141 taaacattgg ggcggcggag ctgaaaggta tggcgctggc gaccatcgtc ggtatcggct
     1201 taagtttgat cttcaaactg attagtatgc tgcgccctga agaagtggtg ctggacgcag
     1261 aagacgcgga tataacagac aaataatgta ccgaccgggc agtgttgctg cccggttcaa
     1321 acatcatggg attctgtggt aaacttctcg cgattttgtg aaatcctggt tgaggtatct
     1381 ctgaacacac cggcacagct ctctttgcca ctttatcttc ctgacgacga aacctttgca
     1441 agtttctggc cgggggataa ctcctcttta ctggccgcgc tgcaaaacgt gctgcgtcag
     1501 gaacatagcg gttacatcta tctctgggca cgcgaaggcg cggggcgcag ccatctgctg
     1561 cacgcggctt gcgcggaatt gtcgcagcgt ggcgatgcgg tgggctatgt cccgctggat
     1621 aaacgcacct ggtttgttcc ggaagtgctc gacggtatgg agcatttgtc gctggtctgt
     1681 attgacaaca ttgagtgtat tgccggcgat gagttgtggg agatggcgat tttcgatctc
     1741 tacaatcgaa ttctggaatc gggcaaaaca cggttgttga tcaccggcga tcgtccaccg
     1801 cggcagttga atctgggatt accggatctc gcgtcgcgtc tcgactgggg gcagatctac
     1861 aaattgcagc cactttctga tgaagataag ttgcaggcgc tacagttacg cgcgcgtttg
     1921 cgtggttttg aactgccgga agatgtgggg cgtttcttgc tgaagcggct cgacagagaa
     1981 atgcgcacgt tgtttatgac gctggatcag ctggatcgtg cgtcgattac cgcgcaacgc
     2041 aagctgacca ttccttttgt gaaagaaatt ctgaagttgt agataaggtg tttattgtcg
     2101 gatgcggcgc gaacgcctta tccgacctac gcgaagttaa tccatagtat tcgtaggccg
     2161 gataagacgc ggcaagcgtc gcatccggca atgtgcttca actagcgatg gagcacatct
     2221 taacccacga tttccagtac ctgctccggt ggacgaccaa tccgtgcttt gccattcgcg
     2281 accacaatcg ggcgttccat tagtttcgga ttatccacca tcgcctgaat cagcgcctct
     2341 tcgctaagtg aactgtcagc caggttcagt tctttataaa gatcctcttt ttggcgcatc
     2401 aattcacgag cgctattcat ccccagcatt ttcagcaaat cacgcagcgt tgccgcatcg
     2461 gcgggtgtct caaggtagag caccacttct ggctccacgc cgttttcttt cagcaggttc
     2521 agcgtttccc ggctctttga acagcgtggg ttatggtaga tttttacctg tttggtcatt
     2581 gttcttcctt taatgcgaat tacatcttgg tataaggctt aaagcgttcc tgcagctggc
     2641 gcaactggtc gatgcgcgca tcgtaacgcg cttgttgcag gctgcctaat ttcacctgcg
     2701 aactggcgct actcaacaag gaaatagcct gatcgagtcg cccggcgagc gcataacctt
     2761 ctgctcgcgc agccagttcc tgatcgcggt tatttagtgc ggcttccgcc tgtgccagta
     2821 aatcccagcc gttgctgtca tctttattgt taaaagtgta gcgattcaga atattggccg
     2881 cttcctgtgg ttgcccccct tgcagataag cgttcgccag gttgagctgc aacaccggat
     2941 tggtgcgcaa atcgcgggca tttttcaggc gattgatagc gtcattggct ttgttttgcc
     3001 caagatcgat atcggtagcc agatcgagat accatgcgtt gccaggttct gctgccagta
     3061 acggttgcag cgttttacgc gcttcgtcgt atttattggc ttccatcgcc tgtagagcac
     3121 gaccatattg cgccgctcgt tgctgacgaa cgtttccttt cgcccattca tccagcaaat
     3181 cactggtgag ctggttacgt ccggaattat acatccccag tgtgcgcgct ttcgccagat
     3241 agaaatcttc cgacgactgc accaccatcg ggcgcatctg attagcacgg ttgcgggcat
     3301 ctgccagacg actttccggc aacgggtgag tcaataaaat ttccggcgga cgcgaggagt
     3361 aacgcgcctg atcgagtaat ttttcgagga aggtcggcat cgcctgcgga tcgaatcccg
     3421 agcgttgcag cacctgaata ccaatgcggt ccgcttcctg ttcattttgc tgggtgaaac
     3481 tgatcatccc ctggcgcgtt cccgccagtg taccggtcag cgccgccatc cccgcctgcg
     3541 gactggccat cgccagtaaa atagaaccta acgcgccgac ccaggtcagc ggcgcgctgc
     3601 gctgctgatc ttccatcgct cgcgccaggt gacgttgggt gacgtgggag atttcgtgcg
     3661 ccataactga agccagttga ctttcgttat cggaataacg gaacagggca gagtgcagca
     3721 ccacgttgcc gccaaagaaa gcaaaggcgt taatttcgtc gttgttgatc agaaagaaat
     3781 gaaacggtgt cttaaccgaa ttggcatgcg aaaccagacg cattcctagc gaattaatat
     3841 attgcgttaa tagcgggtca ttaattaacg gcgcgctgcc gcgtaactgg cggacataat
     3901 agtcgcccat ctgcatttcc tgaccaatgg aaagtgtgct tcctgcggag gttcccatat
     3961 ccggcaaggt gtctgcgctg tcggcaaacg ccggggctac ctgaccaatg gtcatagcag
     4021 caatgagggt tgcaaccagg ttttttttca actgcctgaa cataacctct atcctgtatt
     4081 tctggagata gtcatttgac cgatactgtg aaatatcgtt cccaccgcgc cgcctttgag
     4141 tgtaacgacg aagattagcc cactcaaata acttttcagt ttcctgaatc cgtcataaaa
     4201 agcgaagtct tttttgtgac atttcgatac aattgcggat cgcaatcccg ctcttgaggt
     4261 tcaggtaagg tctgtatgct cgaaatgttg atgcaatggt atcgccgccg ttttagcgac
     4321 ccggaagcga ttgccttgct ggttatttta gtcgccggat ttggcattat ctttttcttt
     4381 agtggcctgc ttgctccgtt gctggtggct attgtgctgg cctatttgct ggagtggcca
     4441 accgtgcgcc tgcaatctat tggctgttcc cgccgctggg cgacgtcgat tgtattggtg
     4501 gttttcgtcg gtatattgct actgatggcg ttcgtggttc tgcctattgc ctggcagcag
     4561 ggcatctact taatccgcga tatgccgggg atgctcaata agctttctga ctttgccgcc
     4621 acgttgccgc gccgctatcc ggcgttaatg gatgcgggca ttattgatgc aatggccgaa
     4681 aatatgcgca gtcggatgct gaccatgggc gattcggtgg tgaaaatttc cctcgcctcg
     4741 ttggtcggtt tgctgaccat tgccgtctat ctggtgctgg tgccattgat ggtcttcttc
     4801 ctgctgaaag acaaagagca gatgctgaac gccgttcgcc gggtgctgcc gcgcaaccgt
     4861 ggactggcag gacaggtgtg gaaggagatg aatcaacaaa tcaccaacta tatccgcggc
     4921 aaagtgctgg agatgatcgt ggtagggatc gccacctggc tggggttctt gctctttggg
     4981 ctgaactatt cgctgctgct ggcggtgctg gtcggcttct cggtacttat tccgtacatt
     5041 ggcgcatttg tggtgaccat tccggtggtt ggcgtggcgc tattccagtt tggtgctggc
     5101 acggaattct ggagctgctt cgcggtgtat ctgattattc aggcgctgga cggcaacctg
     5161 ttagtaccgg tgttgttctc cgaagcggtt aacctgcatc ctctggtgat tattttatcg
     5221 gtggtgatct tcggtggttt gtggggattc tggggcgtat tcttcgctat tccattggcg
     5281 acgctgatca aagccgtgat tcacgcctgg cctgatgggc aaatcgcgca agactgacct
     5341 tcagcgaaaa atttgaaacg agtgttgcag ggcagtgaga agatttgaga tgtgctggcc
     5401 gcaagcgcag ccagcacgga atggagcaaa gtaatcaggc gtgttctttc agccagttca
     5461 gcacaacgtc gtggtgattg ctggttttga aatcgtcaaa gacatgttcg attttgccat
     5521 cagcgtcaat caggaagctg atgcgatgaa taccatcgta ggttttgccc atgaaggact
     5581 tttcacccca gacgccgaat tgttcgcaca cctggtggtc ctcatcagac aggagcgtaa
     5641 agttaagcag ctctttttcc gcaaaacggg agagtttttc gggtttatcg gtgctgatac
     5701 ccagcacatc aacgcccgct tttttcaact catccatgtt atcgcgtaag ccgcaggcct
     5761 gtacggtaca gccgggggtc atggctttcg ggtagaaata aaccagaaca cgctgtccct
     5821 ggaagtcggt caaattaact tgttctccgt cttgatccgg caagctaaat ttcggtgcga
     5881 tatcaccggc tttcagtgga ttcattactt aactccatcc tgttcatcat gttgggaata
     5941 attgacgacg ttaatactgc cttgtgcatt gagttctgta catagggctt tgaacgcttg
     6001 ctcaatattt gctgcgtccg cagatgcggg gctgtgggca gttatctgaa tatgtaactg
     6061 cgcagcccgt tcattttcag caggttgcgt gcgcgacacc agctccgcaa tgttcatatg
     6121 atgcgcgtcg aaaagtgctg tgaagcgttc aattaaatgc ggggagtctg ccacatcgac
     6181 ctgaacccag acagatgctg gcattggtgg acgcggacgc gccgtcgtgc gcttcatcac
     6241 gattaaaaga tccagttcgg cacctttcaa cggtaacgtt gattcaatca gggtgattgc
     6301 attccatgaa ccggaaagca gcatgataaa cgtgaactct tctcccagca tcgccaggcg
     6361 actgtcttca atattacagc cgcaactact gacatgacgg gtgatggtgt tcactattcc
     6421 agggcgatcg gcacccaacg cagtgatcac cagataatgt tgcgatgaca gtgtcaaact
     6481 ggttattcct tcaaggggtg agttgttctt aaggaaagca taaaaaaaac atgcatacaa
     6541 caatcagaac ggttctgtct gcttgctttt aatgccatac caaacgtacc attgagacac
     6601 ttgtttgcac agaggatggc ccatgttcac gggaagtatt gtcgcgattg ttactccgat
     6661 ggatgaaaaa ggtaatgtct gtcgggctag cttgaaaaaa ctgattgatt atcatgtcgc
     6721 cagcggtact tcggcgatcg tttctgttgg caccactggc gagtccgcta ccttaaatca
     6781 tgacgaacat gctgatgtgg tgatgatgac gctggagctg gctgacgggc gcattccggt
     6841 gattgccggg accggtgcta acgctactgc ggaagccatt agcctgacgc agcgcttcaa
     6901 tgacagcggt atcgtcggct gcctgacggt aaccccttac tacaatcgtc cgtcgcaaga
     6961 aggtttgtat cagcatttca aagccatcgc tgaacatact gacctgccgc aaattctgta
     7021 taatgtgccg tcccgtactg gctgcgatct gctcccggaa acggtgggcc gtctggcgaa
     7081 agtaaaaaat attatcggaa tcaaagaggc aacagggaac ttaacgcgtg taaaccagat
     7141 caaagagctg gtttcagatg attttgttct gctgagcggc gatgatgcga gcgcgctgga
     7201 cttcatgcaa ttaggcggtc atggggttat ttccgttacg gctaacgtcg cagcgcgtga
     7261 tatggcccag atgtgcaaac tggcagcaga agggcatttt gccgaggcac gcgttattaa
     7321 tcagcgtctg atgccattac acaacaaact atttgtcgaa cccaatccaa tcccggtgaa
     7381 atgggcatgt aaggaactgg gtcttgtggc gaccgatacg ctgcgcctgc caatgacacc
     7441 aatcaccgac agtggtcgtg agacggtcag agcggcgctt aagcatgccg gtttgctgta
     7501 aagtttaggg agatttgatg gcttactctg ttcaaaagtc gcgcctggca aaggttgcgg
     7561 gtgtttcgct tgttttatta ctcgctgcct gtagttctga ctcacgctat aagcgtcagg
     7621 tcagtggtga tgaagcctac ctggaagcgg caccgcttgc ggagcttcat gccccggctg
     7681 gaatgatttt gccggtgacc tccggcgatt atgcaatccc ggtgaccaac ggtagtggtg
     7741 ctgtcggtaa ggcgctggac attcgtccac cagcccagcc gttggcactg gtttctggcg
     7801 cgcgtaccca gttcacgggc gataccgctt cattgctggt ggaaaatggt cgtggcaata
     7861 ctctgtggcc gcaggtggtt agcgtgctgc aggcgaaaaa ctacaccatc acccaacgtg
     7921 atgatgctgg tcagacactg accaccgatt gggtacaatg gaaccgtctg gacgaagacg
     7981 agcagtatcg tggtcgttat caaatctctg ttaagccgca gggttatcag caggcggtta
     8041 cggttaaact gctgaacctg gaacaggcgg gcaaaccggt tgcagacgcg gcgtccatgc
     8101 agcgttacag cacggagatg atgaacgtta tttccgccgg tctggataaa tctgccactg
     8161 acgccgcgaa cgctgcgcaa aatcgtgcct ccaccactat ggacgtacaa agtgcagctg
     8221 atgacaccgg tttaccaatg ctggtcgtac gcgggccgtt caatgtggtt tggcaacgtc
     8281 tgccagctgc gctggaaaaa gtgggcatga aagtgaccga cagcacccgt tcgcagggca
     8341 acatggccgt aacttataag ccgctgtctg acagcgaatg gcacgaactg ggcgcgagcg
     8401 atccaggtct ggcatccggt gactataaac tgcaggttgg cgatttagat aaccgcagca
     8461 gcctacagtt catcgatccg aaaggtcata ctctgactca gagtcagaac gacgcgctgg
     8521 tagctgtctt ccaggctgcg tttagcaagt aaaaatacag ggctggagtc atccggccct
     8581 tttttctgat atgatacgca aacgtgtgcg tcggcagaaa aacgcgattt tagcggtaat
     8641 tcgcacgaaa tttgtttgtc ggacgtaggt cggataaggc gttcacgccg catccgacaa
     8701 aacatccggc acaccagaca gcaaaagatt ttaaaacgtt aattcacacc caggagtgat
     8761 aaagatgcaa aagcaagctg agttgtatcg tggtaaagcg aaaaccgtat acagcacgga
     8821 aaacccggac ctgttggtgc tcgaattccg caatgatacg tcagcagggg atggcgcgcg
     8881 cattgagcag tttgatcgca aaggtatggt gaacaacaag ttcaactact tcattatgag
     8941 caaactggct gaagcgggta tcccgactca aatggagcgt ctgctctccg ataccgaatg
     9001 tctggtgaaa aagctggata tggtgccggt tgagtgtgtc gtgcgtaacc gtgctgctgg
     9061 ctctctggtg aaacgtcttg gaatcgaaga aggtattgag ctgaacccgc cgctgttcga
     9121 tctgttcctg aaaaacgacg ccatgcacga tccgatggtc aacgaatctt actgcgaaac
     9181 ctttggctgg gtgagcaaag agaacctggc gcgtatgaaa gagctgacct acaaagcgaa
     9241 cgacgtgctg aaaaaactgt tcgatgatgc tggtctgatt ctggtcgact tcaagctgga
     9301 gtttggtctg tacaaaggcg aagtggttct gggcgatgag ttctccccgg acggcagccg
     9361 cctgtgggac aaagaaacgc tggagaaaat ggacaaagac cgtttccgcc agagcctcgg
     9421 tggcctgatc gaagcctatg aagccgtcgc ccgccgcctg ggtgtacagc tggactgatt
     9481 tttctgttca tcatcttgcc gtgctgctgg cacggcaaga caaccgctct cataagatga
     9541 gtattgaacg ctcattcctc cgccatttca tcccgctttc ctgtggtaat cttcctctga
     9601 accacctacg atcatttcta taatgaatat aatgttgagg tatctatgcg ttggcaaggg
     9661 cgacgtgaaa gtgacaatgt tgaagacagg cgcaatagct ccggcggtcc gtcaatgggc
     9721 ggtcccggtt ttcgcctgcc aagcggtaaa ggcgggctga ttttactgat agtcgtgctg
     9781 gttgcaggct actatggcgt tgatttaacc gggttgatga ccggacagcc ggtttcccaa
     9841 cagcaatcaa cgcggtcaat tagccctaat gaagacgaag ccgcaaaatt cacctcggtg
     9901 attctggcaa ccacggaaga cacctgggga cagcaattcg agaagatggg taaaacctat
     9961 cagcaaccga agctggtcat gtaccgtgga atgacgcgta ccggctgcgg ggcgggccag
    10021 tccataatgg ggccattcta ttgcccggcg gatggcacag tttatatcga tctctccttc
    10081 tatgatgaca tgaaagacaa acttggcgcg gatggcgatt ttgcccaggg gtacgttatc
    10141 gctcatgaag tcggtcatca tgtgcagaaa ctgttaggca tcgagccgaa agttcgtcaa
    10201 ctgcaacaaa acgcgacgca ggcggaagta aaccgcttat ctgtgcgtat ggaactccag
    10261 gccgactgtt ttgccggtgt ctgggggcat agtatgcagc agcaaggtgt tctggaaacc
    10321 ggcgatctgg aagaggcgct gaacgcggcg caggccatcg gcgatgaccg tttacaacag
    10381 caaagtcagg ggcgagtagt accagacagt ttcactcatg gcacttctca gcaacgctac
    10441 agctggttta aacgtggttt cgacagcggc gatccggcac aatgcaatac ttttggtaaa
    10501 agcatttaac ttccggggca gggatggctg aactgactgc gcttcacaca ttaacagcgc
    10561 aaatgaaacg tgaagggatc cgccgcttgc tggtgttgag cggggaagag cgttggtgtt
    10621 ttgatcatgc gcttaagttg cgtgatgcct tacctggcga ctggctgtgg atttcgccgc
    10681 agccagatgc tgaaaaccac tgttctccct cggcactaca aactttactt gggcgcgagt
    10741 tccggcatgc ggtattcgac gcccgccacg gctttgatgc cgctgccttt gcggcactta
    10801 gcggaacgtt gaaagcggga agctggctgg tgttgttact ccctgtatgg gatgagtggg
    10861 aaaaccaacc tgatgccgac tcgctgcgct ggagtgattg ccctgaccct attgcgacgc
    10921 cgcattttgt ccagcatttc aaacgcgtac ttacggcgaa taacgacgct atcctctggc
    10981 ggcgaaacca gccgttctcg ttggcgcatt ttactccccg tactgactgg caccccgcta
    11041 ccggcgcacc acaaccagaa caacagcaac tcttacagca gctactgacc atgccgccgg
    11101 gtgtggcagc ggtaactgct gcgcgtgggc gcggtaagtc ggcgttggca gggcaactca
    11161 tttctcgtat tgcgggcagt gcgattgtca ccgctcccgc aaaagcggca acggatgtac
    11221 tggcacaatt tgcgggcgag aagtttcgct ttattgcgcc ggatgccttg ttagccagcg
    11281 atgagcaagc cgactggctg gtggtcgatg aagccgcagc tatacctgcg ccgttgttgt
    11341 atcaactggt atcgcgtttt cctcgaacgt tgttaactac tacggtgcag ggctacgaag
    11401 gcaccggacg tggttttttg ctgaaatttt gcgctcgctt tccgcattta caccgttttg
    11461 aactacaaca gccgatccgt tgggcgcagg gatgcccgct ggaaaaaatg gttagtgagg
    11521 cactggtgtt tgacgatgaa aacttcaccc atacaccaca aggcaatatc gttatttccg
    11581 catttgaaca gacgttatgg cgaagcgagc cagaaacgcc gttaaaggtt tatcagttat
    11641 tgtctggtgc gcactaccgg acctcgccgc tggatttacg ccgcatgatg gatgcaccag
    11701 gtcaacattt tttacaggcg gctggcggaa acgagattgc cggagcgctg tggctggtgg
    11761 atgagggcgg attatctcaa gaactcagtc aggcggtatg ggcgggtttt cgtcgcccgc
    11821 ggggtaatct ggtggcccag tcgctggcgg cgcacggcag caatccactg gcggcgacat
    11881 tgcgcggacg gcgggttagc cggatagcag ttcatccgac tcgtcagcgg gaaggcacag
    11941 ggcgacagct tattgctggt gctttgcaat atattcatga cctcgactat ctttcggtga
    12001 gttttggtta caccgaggag ttatggcgtt tctggcaacg ctgcggtttt gtgctggtgc
    12061 gaatgggtaa tcatcgtgaa gccagcagcg gttgctatac ggcgatggcg ctgttaccga
    12121 tgagtgatgc gggtaaacag ctggctgaac gtgagcatta ccgtttacgt cgcgatgcgc
    12181 aagctctcgc gcagtggaat ggcgaaatgc ttcctgttga tccactaaac gatgccgtcc
    12241 tttctgacga cgactggctt gaactggccg gttttgcttt cactcatcgt ccgctattaa
    12301 cgtcgttagg ttgcttattg cgtctgctac aaaccagcga actggcatta ccggcgctgc
    12361 gtgggcgttt acagaaaaac gccagtgacg cgcagttatg taccacactt aaactttcag
    12421 gccgtaagct gttactggtt cgccagcggg aagaggccgc gcaggcgcta ttcgcacttg
    12481 atgatgttcg cactgagcgt ctgcgcgatc gcataacgca atggcaattt tttcactgac
    12541 tccttcaatt ttctcccatg gtcattgtga gtcagcggcg taaaattaat gacatcagtt
    12601 aaggagttcg ccatgaaaca tgaccatttt gttgttcaaa gcccggataa accagcacaa
    12661 cagttgttac tgcttttcca cggtgtcggg gataacccgg tggcaatggg ggaaataggt
    12721 agctggtttg cgccgctgtt tcctgatgcg ctggtggtga gtgtcggtgg cgcggagccg
    12781 agcggtaatc cggcggggcg tcagtggttt tcggtgcagg gtattacgga agataatcgc
    12841 caggcgcgtg tgaatgccat catgccgacg tttattgaga cggtgcgcta ctggcagaaa
    12901 caaagcggag taggggctaa cgccacggcg ctaatcggtt tctcacaagg cgcgattatg
    12961 gcgctggaga gcattaaagc cgaaccgggc cttgcttcgc gcgtcattgc cttcaacgga
    13021 cgctacgcga gcctgccgga aaccgcgtct acagccacaa caattcactt gattcatggc
    13081 ggtgaagatc cggttatcga tctggcgcat gcggttgcgg cgcaggaagc gttaatcagt
    13141 gccggtggtg acgtgacgct ggatatcgtg gaggatttag gccatgcgat cgacaatcgc
    13201 agtatgcaat ttgccctgga tcacttgcgt tataccattc cgaaacatta tttcgacgaa
    13261 gcgctaagcg gcggtaagcc tggcgatgat gatgtgattg aaatgatgta ataatagccg
    13321 ggtaggtctg ataagactgt atcaggcatc atggccggat gcggcgttaa cgccttatcc
    13381 ggccacttag cttacttctt cggttgatcc tttttcggcc agtcgtcgtc atcgtcccac
    13441 ttatcgttga aatcacgatg cgggggaagc tccggcttgt tggcgagaaa tttcttatgg
    13501 tcgacgcgct tgagatcttt aatcacgttc agcaggacgc ctaccaaaaa gacaatcacc
    13561 agaatccacc aatatttagc cagccagtcc atgcttattt cctcttgcag aaccactcat
    13621 caggcgacga gctgttccat gatacgttga tacatacggg caagtagctg caggtcggca
    13681 gcgttcacac attcattgat tttatgaatc gtggcattga ccggcccgag ttccaccacc
    13741 tgcgccccca tgcgggcaat aaagcgcccg tcggacgttc cgcctgtggt cagtagctgc
    13801 ggtttaattt cattatagtg ctcaaccgcg ttaacgaccg catccaccag tttaccgcgc
    13861 gcggtcaaaa atggctgccc ggaaagccac caatccaccg tatagcgcag ttgatgtttt
    13921 tcaagcaggg caagcacctg cgctttgatc atctcatccg tcagttcggt gctaaagcgg
    13981 aagttaaact gcacaaacag ttcacctgga ataacgttgt tactgcccgt tcccgcctga
    14041 atattggcaa tctgcatact ggtcgccggg aagaagtcat taccctgatc ccactcaata
    14101 gccactaatt cattaatgaa aggtgctgcg cgatgtaccg gattgtcagc cagatgtggg
    14161 taggcaacat gcccctgaac gccatgaatg gtaaggttgc aggttaacga tccgcgacga
    14221 ccatttttca ccacatcacc taccacttcg atactcgacg gttcgccaac cagacagtaa
    14281 tcgagacgct cattacgcgc cattaacgct tcgacgactt ttaccgtacc gttgtgggca
    14341 ctagcttctt catcagaggt gatcagaaat gccagtcgcc ctgtatggtt gggatgttgt
    14401 gcgacaaagc gttctgccgc taccaccatc gccgccagcg agcctttcat atctgccgca
    14461 cctcgcccaa ataacatgcc gtcacgaatg gttggttcaa atggtggatt gatccaacgg
    14521 tcggcgtcgc caggcggcac cacgtcggta tgcccggcaa aggctaacgt ttcaccctgc
    14581 ccgcgccatg cccaaaaatt ctgcgtatcg gcaaagtcca tgcgttcaac ggtaaaaccg
    14641 atcgcctgca aacgttcaat caacaaagcc tggcatcctg cgtcatcagg actcagggaa
    14701 gggcggcgaa taagctgttg tgtcagctca ataaccgggc acgacataga ctacacctca
    14761 tggaaaaatt gctgataact ggattcactg aaacccagca gcataggctt accaggcgcg
    14821 cagagcaatg gacgtttgat aattgcaggc atttcagtca ttaatgtcgc cgcagaggcc
    14881 gcatcggtga ttttattgcg ggtggtttcg tccagtttac gccaggttgt accacgggtg
    14941 ttgagtaacg cttcccagcc taattcgttg ataaaaccgt tcaataattc gctgtccaat
    15001 ccatcgacgc ggtaatcatg aaaacgatag tcgatgttat tggcttccag ccaacggcga
    15061 gcctttttaa tggtgtcaca atttttgatg ccgtaaagtg taaccataat gaatcctttt
    15121 tcgatttatg agaataaata ctcatttaag ggtgaaaaca ttttgcctgc aaatgattat
    15181 ca
//
LOCUS       DAHHTG010000104        15113 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.105, whole genome shotgun sequence.
ACCESSION   DAHHTG010000104 DAHHTG010000000
VERSION     DAHHTG010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15113)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15113)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15113
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.105"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            30..770
                     /locus_tag="NBI74_003346"
     CDS             30..770
                     /locus_tag="NBI74_003346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
                     /protein_id="HCD1933629.1"
                     /translation="MFFNTKYTAALCFATCVAFSSSAIADIVISGTRVIYKSDQKSVN
                     IRLENKGNNPLLVQSWLDTGDDNAEPGSITVPFTATPPVSRIDAKRGQTIKLMYTASS
                     VLPKDRESVFWFNVLEVPPKPDAATVANQSLLQLAFRTRIKLFYRPEALKGNPSEAPL
                     ALKWSWSGSEGKAALRVANPTPYYVSFSSGDLEASGKRFPIDVKMIAPFSDEVMKVTG
                     LNGKANSAKVHFYAINDFGGAIEGNASL"
     gene            805..3405
                     /gene="htrE"
                     /locus_tag="NBI74_003347"
     CDS             805..3405
                     /gene="htrE"
                     /locus_tag="NBI74_003347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Yad fimbria usher protein HtrE"
                     /protein_id="HCD1933630.1"
                     /translation="MTIKPTKNYHNHLTRIATFCALLYCNGAFCAELVEYDHTFLMGQ
                     NASNIDLSRYSEGNPAIPGVYDVSVYVNDQPVINQSITFVAIEGKKNAQACITLKNLL
                     QFHINSPDINSEKAVLLARDEALGNCLNLTEIIPQASVRYDVNDQRLDIDVPQAWVMK
                     NYQNYVDPSLWENGINAAMLSYNLNGYHSETPGRKNDSIYAAFNGGMNLGAWRLRASG
                     NYNWMTDSGGNYDFKNRYIQRDIASLRSQFIIGESYTTGETFDSVSIRGIRLYSDSRM
                     LPPTLASFAPIIHGVANTNAKVTITQGGYKIYETTVPPGAFVIDDLSPSGYGSDLIVT
                     IEESDGSKRTFSQPFSSVVQMLRPGVGRWDISGGQVLKDDIQDEPNLFQASYYYGLNN
                     YLTGYTGIQITDNNYTAGLLGLGLNTSVGAFSFDVTHSNVRIPDDKTYQGQSYRVSWN
                     KLFEETSTSLNIAAYRYSTQNYLGLNDALTLIDEVKHPEQDLEPKSMRNYSRMKNQVT
                     VSINQPLKFEKKDYGSFYLSGSWSDYWASGQNRSNYSIGYSNSASWGSYSISAQRSWN
                     EDGDADDSVYLSFTIPIEKLLGTEQRTSGFQSIDTQMSSDFKGNNQLNVSSSGYNDNA
                     RVSYSVNTGYTMNKASKDLSYVGGYASYESPWGTLAGSVSANSDNSRQVSLSTDGGFV
                     LHSGGLTFSNDSFSDSDTLAVVQAPGAQGARINYGNSTIDRWGYGVTSALSPYHENRI
                     ALDINDLENDVELKSTSAVAVPRQGSVVFADFETVQGQSAIMNITRSDGKNIPFAADI
                     YDDQGNVIGNVGQGGQAFVRGIEQQGNINIKWLEESKPVSCLAHYQQSSEAEKIAQSI
                     ILNGIRCQIQ"
     gene            3422..3994
                     /locus_tag="NBI74_003348"
     CDS             3422..3994
                     /locus_tag="NBI74_003348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414680.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCD1933631.1"
                     /translation="MIKTTPHKIAILMGVLLSPSVFATDINVEFTATVKATTCNITLT
                     GNNVTNDGNNNYTLRIPKMGLDKITNKTTESQADFKLVASGCSSGISWIDTTLTGNAL
                     SSSSKLIIPLSGDLSSTTSNIGMGFKKRTTDDATFLQPNNSADKIRWSTEEMQPDKGL
                     EMTVALRETAAGQGVPGIFRALATFNFIYQ"
     gene            4009..4611
                     /gene="yadL"
                     /locus_tag="NBI74_003349"
     CDS             4009..4611
                     /gene="yadL"
                     /locus_tag="NBI74_003349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000143198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like protein YadL"
                     /protein_id="HCD1933632.1"
                     /translation="MTFKNLRYGLSSSVVLAASLFSVPSYAATDSIGLTVITTVEMGT
                     CTATLVNDSDQDISVVDFGDVYISEINAKTKVKTFKLKFKDCAGIPNKKAQIKLTKRA
                     TCEGTANDGAGFANGSTATDKASAVAVEVWSTETPATGRATQFSCVTPASQEVTISTA
                     ANAVVYYPMSARLVVEKNKTVSNVTAGKFSAPATFTVTYN"
     gene            4638..5234
                     /locus_tag="NBI74_003350"
     CDS             4638..5234
                     /locus_tag="NBI74_003350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCD1933633.1"
                     /translation="MHPTQRKLMKRIILFLSLLFPITCPAIAGQDIDLVANVKNSTCK
                     SGISNQGNIVLGVVGVGYFSGNVTPESYQPGGKEFTVTVSDCALQGTGDVLNQLHIDF
                     RALSGVMAAGSRQIFANEVTTGAKNVGVVLFSIQDPANIFNVLSSAGNSRSIYPVMTS
                     ALNNSSWKFYARMQKIDPALDVISGQVMSHILVDVHYE"
     gene            5286..6539
                     /locus_tag="NBI74_003351"
     CDS             5286..6539
                     /locus_tag="NBI74_003351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001462196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like adhesin"
                     /protein_id="HCD1933634.1"
                     /translation="MKTFFKYFVFLVLSCSCFTAISKDTSFLVGNNAGVGDGNFNGPS
                     TAAQVTFRYASTTNNLVFYKPTQLGPTGVKLRWEQLDTASGGGFLYCNASDRANPDGL
                     MDIENAMADSGKTYGGHKLFKTSVPGLYYTLFIAKLWSAYSTKTTISDSGLYIGDTTP
                     QKFQWIITDPDLQHIGCDNAMRYDRFWAIGGVVHDLTIEFYTDTDFNPTTNQDVSLSS
                     NSTYLYAFKAYSPGSRVVDHSHHLYIDFDLLNVKLTNPTCFTAVLTGKSVSGSTVKMG
                     EYAPGQIKNGATPVPFDISLQNCVRVGDIETKLSSGKLGTENKQLLGNTLTGSGAAKG
                     VGVLIEGLANRKSALMILKPNDSTSVYKDNTGQAQDHDSDAIYPEDQGITYPLHFQAT
                     LKQDGNIAIEPGEFKATSTFQVTYP"
     gene            6652..7446
                     /gene="panB"
                     /locus_tag="NBI74_003352"
     CDS             6652..7446
                     /gene="panB"
                     /locus_tag="NBI74_003352"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000805487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HCD1933635.1"
                     /translation="MKPTTIASLQKCKQEKKRFATITAYDYSFAKLFAEEGLNVMLVG
                     DSLGMTVQGHDSTLPVTVADIAYHTAAVRRGAPNCLLLADLPFMAYATPEQAFENAAT
                     VMRAGANMVKIEGGEWLVETVQMLTERAVPVCGHLGLTPQSVNIFGGYKVQGRGDEAA
                     DRLLSDALALEAAGAQLLVLECVPVELAKRITEALAIPVIGIGAGNVTDGQILVMHDA
                     FGITGGHIPKFAKNFLAETGDIRAAVRQYMAEVESGVYPGEEHSFH"
     gene            7458..8309
                     /gene="panC"
                     /locus_tag="NBI74_003353"
     CDS             7458..8309
                     /gene="panC"
                     /locus_tag="NBI74_003353"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HCD1933636.1"
                     /translation="MLIIETLPLLRQQIRRLRMEGKRVALVPTMGNLHDGHMKLVDEA
                     KARADVVVVSIFVNPMQFDRPEDLARYPRTLQEDCEKLNKRKVDLVFAPSVKEIYPNG
                     TETHTYVDVPGLSTMLEGASRPGHFRGVSTIVSKLFNLVQPDIACFGEKDFQQLALIR
                     KMVADMGFDIEIIGVPIMRAKDGLTLSSRNGYLTAEQRKIAPGLYKVLSSIADKLQAG
                     ERDLDEIIAIAGQELNEKGFRADDIQIRDADTLLEVSENSKRAVILVAAWLGDARLID
                     NKMVELA"
     gene            complement(8391..8594)
                     /locus_tag="NBI74_003354"
     CDS             complement(8391..8594)
                     /locus_tag="NBI74_003354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000404125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCD1933637.1"
                     /translation="MEIKTRRETRQTLAQWFEEKGFQKGYKEGLQKVRLAQRLLSKGM
                     SREDVAELANLPLAEIDKLINSN"
     gene            complement(8663..9595)
                     /locus_tag="NBI74_003355"
     CDS             complement(8663..9595)
                     /locus_tag="NBI74_003355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HCD1933638.1"
                     /translation="MDAPSTTPHDAVFKQFLMHAETARDFLDIHLPAELRELCDLDTL
                     HLESGSFIEESLKGHSTDVLYSVQMQGNPGYLHVVIEHQSKPDKKMTFRMMRYAIAAM
                     HRHLEADHDKLPLVVPILFYQGEATPYPLSMCWFDMFYSPELARRVYNNPFPLVDITI
                     TPDDEIMQHRRIAILELLQKHIRQRDLMLLLEQLVTLIDEGYTSGSQLVAMQNYMLQR
                     GHTEQADLFYGVLRDRETGGESMMTLAQWFEERGRQEERQEVRQEVRQEVIQEVRQEV
                     RQEFALRFLSKGMSREDVAEMANLPLAEVDKLIS"
     gene            9869..10249
                     /gene="panD"
                     /locus_tag="NBI74_003356"
     CDS             9869..10249
                     /gene="panD"
                     /locus_tag="NBI74_003356"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HCD1933639.1"
                     /translation="MIRTMLQGKLHRVKVTHADLHYEGSCAIDQDFLDAAGILENEAI
                     DIWNVTNGKRFSTYAIAAERGSRIISVNGAAAHCASVGDIVIIASFVTMPDEEARTWR
                     PNVAYFEGDNEMKRTAKAIPVQVA"
     gene            complement(10253..11482)
                     /locus_tag="NBI74_003357"
     CDS             complement(10253..11482)
                     /locus_tag="NBI74_003357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HCD1933640.1"
                     /translation="MYKQAVILLLMLFTASVSAALPARYMQTIENAAVWAQIGDKMVT
                     VGNIRAGQIIAVEPTAASYYVFNFGFGKGFIDKGHLEPVQGRQKVEDGLGDLNKPLSN
                     QNLVTWKDTPVYNAPSVGSAPFGVLADNLRYPILHKLKDRLNQTWYQIRIGDRLAYIS
                     ALDAQPDNGLPVLTYHHILRDEENTRFRHTSTTTSVRAFNNQMAWLRDRGYATLSMAQ
                     LEGYVKNKINLPARAVVITFDDGLKSVSRYAYPVLKQYGMKATAFIVTSRIKRHPQKW
                     NPKSLQFMSVSELNEIRDVFDFQSHTHFLHRIDGYRRPILLSRSEHNILFDFARSRRA
                     LAQFNPHVLYLSYPFGGFNDKAVKAAKEAGFHLAVTTMKGKVKPGDNPLLLKRLYILR
                     TDSLETMSRLVSNQPQG"
     gene            complement(11546..11986)
                     /locus_tag="NBI74_003358"
     CDS             complement(11546..11986)
                     /locus_tag="NBI74_003358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HCD1933641.1"
                     /translation="MLGWVITCHDDRAQEILDALEKKHGALLQCRAVNFWRGLSSNML
                     SRMMCDALHETDSGEGVIFLTDIAGAPPYRVASLLSHKHSRCEVISGVTLPLIEQMMA
                     CRETMTSSAFRERIVELGAPEVSSLWHQQQKNPPFVLKHNLYEY"
     gene            complement(12091..12861)
                     /locus_tag="NBI74_003359"
     CDS             complement(12091..12861)
                     /locus_tag="NBI74_003359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001747261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCD1933642.1"
                     /translation="MMHLYWVALKSIWAKEIHRFMRIWVQTLVPPVITMTLYFIIFGN
                     LIGSRIGDMHGFSYMQFIVPGLIMMSVITNAYANVASSFFGAKFQRNIEELLVAPVPT
                     HVIIAGYVGGGVARGLLVGMLVTAISLFFVPFQVHSWVFVALTLVLTAVLFSLAGLLN
                     GVFAKTFDDISLVPTFVLTPLTYLGGVFYSLTLLPPFWQGLSHLNPIVYMISGFRYGF
                     LGINDVPLVTTFGVLVVFIVAFYLICWSLIQRGRGLRS"
     gene            complement(12858..13784)
                     /locus_tag="NBI74_003360"
     CDS             complement(12858..13784)
                     /locus_tag="NBI74_003360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001621186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCD1933643.1"
                     /translation="MTIALELQQLKKTYPGGVQALRGIDLQVEAGDFYALLGPNGAGK
                     STTIGIISSLVNKTSGRVSVFGYDLEKDVVNAKRQLGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVERKEAYIRSEKYLKQLDLWGKRNERARMLSGGMKRRLMIARALMHEPKLLIL
                     DEPTAGVDIELRRSMWGFLKDLNDKGTTIILTTHYLEEAEMLCRNIGIIQHGELVENT
                     SMKALLAKLKSETFILDLAPKSPLPKLDGYQYRLVDTATLEVEVLREQGINSVFTQLS
                     EQGIQVLSMRNKANRLEELFVSLVNEKQGDRT"
     gene            13893..14555
                     /gene="can"
                     /locus_tag="NBI74_003361"
     CDS             13893..14555
                     /gene="can"
                     /locus_tag="NBI74_003361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonate dehydratase"
                     /protein_id="HCD1933644.1"
                     /translation="MKDIDTLISNNALWSKMLVEEDPGFFEKLAQAQKPRFLWIGCSD
                     SRVPAERLTGLEPGELFVHRNVANLVIHTDLNCLSVVQYAVDVLEVEHIIICGHYGCG
                     GVQAAVENPELGLINNWLLHIRDIWFKHSSLLGEMPQERRLDTLCELNVMEQVYNLGH
                     STIMQSAWKRGQKVTIHGWAYGIHDGLLRDLDVTATNRETLEQRYRHGISNLKLKHAN
                     HK"
     gene            complement(14596..>15113)
                     /gene="hpt"
                     /locus_tag="NBI74_003362"
     CDS             complement(14596..>15113)
                     /gene="hpt"
                     /locus_tag="NBI74_003362"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004204384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HCD1933645.1"
                     /translation="MIPEAEIKARIAELGRQITERYKDSGSDMVLVGLLRGSFMFMAD
                     LCREVQVSHEVDFMTASSYGSGMSTTRDVKILKDLDEDIRGKDVLIVEDIIDSGNTLS
                     KVREILSLREPKSLAICTLLDKPSRREVNVPVEFIGFSIPDEFVVGYGIDYAQRYRHL
                     PYIGKVILLDE"
BASE COUNT         4113 a         3668 c         3441 g         3891 t
ORIGIN      
        1 tgttttttta actctaccag gaatggttta tgtttttcaa cactaaatat acagcagcat
       61 tatgcttcgc aacctgtgta gcttttagct cgtcagctat agcagatatc gtaatttccg
      121 gtacacgcgt aatctataaa agcgaccaaa aaagtgtcaa cattcgcctg gaaaataaag
      181 gcaataaccc attacttgtt cagagttggc tggatactgg tgatgacaat gctgagcctg
      241 gcagtattac cgtccccttt actgcgactc cgcccgtatc acgcattgat gcaaaacgcg
      301 gccaaaccat caaactgatg tacaccgcca gcagcgtcct gccaaaagac agagaaagtg
      361 tgttctggtt taacgtacta gaagttcctc caaaaccaga tgccgcaaca gttgcaaatc
      421 aaagcctgct gcaattggca tttcgcacac gtataaaact gttctatcgc ccggaagcat
      481 tgaaggggaa tccctcagaa gcaccattag ccctgaaatg gtcctggtca ggttccgagg
      541 gtaaagcagc attacgagtg gccaatccaa ccccttacta cgtctctttt agcagtggtg
      601 atttagaagc cagcggtaaa cgctttccga ttgatgtgaa aatgattgca ccattcagtg
      661 atgaagtcat gaaagtcact ggccttaatg gcaaagcaaa ttctgcaaaa gtgcattttt
      721 acgccattaa tgattttggt ggtgccattg aaggtaatgc cagtctgtaa tcaggcaagg
      781 atataattcc gcaggaagca taacgtgact ataaaaccaa ctaaaaatta tcacaatcat
      841 ctgacccgta tcgccacgtt ttgcgcgctg ctgtattgca atggtgcttt ctgtgctgaa
      901 cttgttgaat atgaccatac cttcctgatg gggcagaatg cctcaaatat tgatctcagc
      961 cggtatagtg aaggtaaccc cgctataccg ggtgtgtacg acgttagtgt ttatgtcaac
     1021 gatcaacctg ttattaacca aagcattaca tttgtcgcaa ttgaaggaaa aaagaacgca
     1081 caagcctgta tcacacttaa aaatttattg cagttccata ttaattctcc cgatataaac
     1141 agcgaaaaag ccgttctgct tgcccgggat gaagctctcg gcaattgcct caatttgacg
     1201 gaaattatcc ctcaggcttc cgttcgttat gacgttaacg atcaacgtct ggatatagac
     1261 gttcctcagg cctgggtaat gaaaaattac caaaactatg ttgatccatc gttatgggaa
     1321 aatggcatta atgcagccat gttgtcatac aacctcaacg gatatcatag tgaaacccct
     1381 ggtcgaaaaa atgacagcat ttatgctgct tttaacggtg ggatgaattt gggtgcatgg
     1441 cggctgcgtg cctcaggcaa ctacaactgg atgaccgatt ctggcggtaa ttatgatttt
     1501 aagaatcggt atatacagcg tgatatcgcc tcactacgct ctcaattcat tattggtgag
     1561 tcttatacga cgggcgaaac ctttgattcc gtcagtatcc gaggcattcg cttatacagt
     1621 gacagccgca tgttgcctcc gacattagcc agctttgcgc ctatcattca tggcgttgcc
     1681 aataccaacg ccaaagtcac tattacgcaa ggtggctata agatttatga aacgacggtg
     1741 ccgccaggcg ctttcgtcat tgatgatctg agtccatcag ggtacggcag tgatcttatt
     1801 gttaccatcg aagaatccga tggctcaaag cggacattct cgcaaccttt ttcatcggtc
     1861 gttcaaatgt tacgccctgg cgttggacgt tgggatatta gcggcggcca ggtcttaaaa
     1921 gatgatattc aggatgagcc taatttattt caggccagct actactacgg tctgaataac
     1981 tatctgacgg gttataccgg tattcagatt accgataata actataccgc tggtttgtta
     2041 ggtcttggtc tgaatacttc agtcggtgca ttttctttcg atgtgactca ctccaatgtt
     2101 cgtataccgg atgataaaac ataccagggg caaagttatc gtgtttcctg gaacaagtta
     2161 ttcgaagaaa caagtacttc actgaatatc gcggcctatc gctattcgac acagaattat
     2221 cttggtctta atgatgcact aactctgatt gatgaagtga aacatccaga acaagatctt
     2281 gaaccgaaat ccatgcgtaa ttactcgcgc atgaaaaatc aggttacagt cagtattaac
     2341 caaccgttga aatttgaaaa aaaagattac ggttcttttt atctttctgg tagttggtcc
     2401 gattactggg cttccggaca aaatcgtagc aattactcca ttggctacag taacagtgca
     2461 tcctggggca gctacagcat cagtgcacag cgttcatgga atgaggacgg cgacgctgat
     2521 gatagcgttt atctcagttt caccattcct attgaaaaat tacttggcac tgaacaacgt
     2581 acttcaggtt tccagagtat cgatactcaa atgagcagtg actttaaggg taataaccaa
     2641 ctcaacgtca gtagcagtgg ctataacgat aacgctcgcg tcagttatag cgtgaatact
     2701 ggctatacga tgaataaagc cagcaaagat ttgagttatg ttgggggtta tgccagctat
     2761 gaatcaccgt ggggaactct ggcaggttca gtttctgcaa acagcgataa cagtcgtcaa
     2821 gtttctctca gcaccgacgg tggttttgta ttgcatagtg gcggcctgac cttcagtaat
     2881 gacagtttta gcgactccga tacactggcg gtagttcagg ctccaggtgc tcaaggagcg
     2941 cgaataaatt atggcaacag tactatcgat cgatggggtt atggtgtcac cagcgctctt
     3001 tctccttatc atgaaaaccg tatcgcgctg gatatcaacg atcttgagaa cgatgttgaa
     3061 ttaaaaagta ccagtgctgt agctgtgcct cgtcagggtt ctgtcgtttt tgctgatttt
     3121 gaaaccgtgc aagggcaatc agccattatg aacatcacac gtagtgatgg taaaaatatt
     3181 ccatttgctg cagatattta tgatgaccaa ggcaatgtca ttggtaatgt tggacagggt
     3241 ggacaggctt ttgttcgtgg tattgaacag caaggaaata tcaacattaa atggctcgaa
     3301 gaaagtaaac ctgtaagttg tcttgcgcat tatcaacaaa gttcagaagc agaaaaaata
     3361 gcgcaatcta ttattctgaa tggaattcgg tgtcagattc agtaactaca agggacgtca
     3421 aatgataaaa acaacgccac ataaaatagc gatactgatg ggagtattat tatcaccctc
     3481 agtatttgca acggatatta atgttgagtt tacagccact gtcaaagcga caacctgtaa
     3541 catcacactt actggtaata atgtcacgaa tgatggcaat aataactaca ctttgagaat
     3601 ccctaagatg ggcctggata agatcacgaa taaaacgaca gaatctcagg ctgattttaa
     3661 actggttgcc agtgggtgca gcagtggcat tagctggatt gataccactc tgaccggaaa
     3721 cgcattatca agctcatcca agcttattat accgctgtct ggtgatttat cttcgacgac
     3781 aagtaatatc ggtatgggtt tcaaaaaacg gactactgat gatgccactt tccttcaacc
     3841 taacaacagt gcggacaaga tacgctggag cacagaagaa atgcagcccg ataaaggtct
     3901 tgaaatgact gtcgcgctac gtgaaaccgc agcagggcaa ggcgtgccgg ggatttttcg
     3961 cgccctggcg acgtttaatt tcatctatca ataagaagga taattaagat gacatttaaa
     4021 aatttacgtt atggattatc cagcagtgtc gttttggctg cctcattgtt cagcgtaccc
     4081 tcttatgcgg caactgacag cattgggctg accgtgatta ccaccgtaga aatgggtact
     4141 tgtaccgcta cattagtaaa tgactctgat caggacattt ctgttgttga ttttggtgat
     4201 gtatatattt ctgaaatcaa tgccaagacc aaagtaaaaa cattcaaact caaattcaaa
     4261 gactgtgcgg gtatccccaa taaaaaagcg caaataaaat taaccaagcg agccacatgc
     4321 gaggggactg ctaatgacgg tgcagggttc gcaaatggtt ccacagccac agataaagca
     4381 agtgccgtcg ccgttgaagt ctggagcacc gaaactccgg caacagggag agcaacacaa
     4441 tttagttgtg tgacaccagc atctcaagag gtaactattt ccactgctgc caatgcggtt
     4501 gtttattacc cgatgagtgc tcgcctggtc gttgaaaaaa ataaaaccgt aagtaatgtc
     4561 actgcaggta aattttctgc acctgccaca tttacagtaa cctataacta acagactgac
     4621 agattcagga actatatatg catcccactc aacgtaagct gatgaagaga ataattctgt
     4681 ttctgtcatt actgtttccc attacctgtc cagcgatcgc tggacaggat attgaccttg
     4741 ttgctaatgt aaaaaacagc acttgcaaaa gcggaatcag taaccagggt aatattgttc
     4801 ttggtgtcgt tggggtggga tatttttcag gtaatgttac tcctgaaagc tatcaaccag
     4861 gtggaaaaga gttcactgtc actgtctcag actgtgcatt acagggaact ggcgatgtgc
     4921 taaatcagtt acatattgat tttagagccc ttagcggtgt catggctgca ggctccaggc
     4981 aaatatttgc caatgaagtg acaaccgggg ctaaaaatgt cggtgtcgtt ctcttttcca
     5041 ttcaggaccc ggccaatata ttcaatgttc ttagttcagc gggaaattct cgttcaatct
     5101 atccagtaat gacttctgcc ctgaataatt cgtcatggaa attttatgcc agaatgcaaa
     5161 aaatcgatcc cgcgctggat gtcatatctg gccaggtgat gagtcatata ttagttgatg
     5221 tgcactacga ataatatatc aaaacaaacg actgctatta tgacagcagc gaatagtggg
     5281 taatcatgaa gacattcttc aaatactttg tttttttagt actatcctgc tcttgcttta
     5341 cagcgattag taaggataca agttttcttg ttggtaacaa tgccggggta ggcgatggta
     5401 atttcaacgg cccgagcact gcggctcagg tcacttttag atatgcatca acaaccaata
     5461 atttagtttt ttataaacct actcaacttg gcccgacagg tgttaaatta cgctgggaac
     5521 aattagatac agccagcggt ggcggttttc tttactgtaa tgcctctgac agggcaaatc
     5581 cagatggact aatggatatt gaaaatgcca tggctgattc tggtaaaacc tatggtggac
     5641 ataagttatt taaaacctct gtcccaggct tgtattacac tctatttatt gcaaaacttt
     5701 ggtctgcata ttcaacaaaa acaactatta gcgactctgg tttatatatt ggagatacca
     5761 caccacaaaa attccagtgg atcatcacag accctgatct acaacatatc ggctgcgata
     5821 acgcgatgcg gtacgataga ttctgggcca tcggcggtgt tgtacacgat cttacgattg
     5881 aattttatac tgataccgat tttaacccta caacaaacca ggacgtatca ttgtccagta
     5941 attcgacata tctttatgcg tttaaggcct acagtcccgg ttctcgtgtt gttgatcaca
     6001 gccatcacct ttatatcgac tttgatctac tcaatgttaa attaactaac cccacctgct
     6061 ttactgctgt acttaccgga aaatcagtaa gtggttcgac ggtaaaaatg ggagaatatg
     6121 caccagggca gattaaaaat ggtgctactc cggttccttt tgatatttct ctccagaact
     6181 gtgttcgcgt tggcgatata gagacaaagc tatcttcagg gaagctgggt acagaaaata
     6241 agcaactcct gggtaacact cttactggaa gcggcgcagc aaaaggcgtt ggggtattga
     6301 ttgaaggatt agcaaacaga aaaagtgccc taatgatact gaaaccgaat gattcaactt
     6361 ctgtttataa agataatacg ggtcaggctc aggatcacga ttcagatgca atttacccag
     6421 aagatcaagg tataacatat ccacttcatt tccaggcgac gttaaagcaa gacggaaata
     6481 ttgctatcga accaggagaa tttaaagcca ccagtacttt ccaggtaacc tatccctgat
     6541 aacattgtgg caccgtctgg taatggcggt gccacaatag ataaatcatt gcccccacca
     6601 atgtggctga taaaatgcca gacagcacga cattacttat caggatacgt tatgaaacca
     6661 accactattg cttcattgca aaaatgcaaa caagaaaaaa aacgtttcgc gaccatcact
     6721 gcttacgatt atagcttcgc aaaactgttt gccgaagaag gacttaacgt catgctggtc
     6781 ggcgattcac tgggcatgac ggttcagggg catgactcca cgctaccggt taccgttgct
     6841 gatatcgcct accacactgc cgccgtgcgt cgcggcgcgc caaactgcct tctgttagca
     6901 gacctgccat ttatggcgta tgccacgccg gaacaagcct ttgaaaacgc cgcaacggta
     6961 atgcgtgccg gagccaacat ggtcaaaatt gaaggcggtg agtggctggt agaaacagta
     7021 caaatgctga ccgaacgtgc cgttccagtt tgtggtcatt taggcctaac cccacagtca
     7081 gtaaatattt tcggtggcta caaagtccag gggcgcggcg atgaagcggc cgatcgactg
     7141 ctcagcgatg cattagcctt agaagctgct ggggcacaac tgctggtact ggagtgcgtg
     7201 ccggttgaac tggcgaaacg cattaccgaa gcactggcga tcccggttat cggtattggc
     7261 gcaggcaacg tcactgacgg gcaaatcctc gtgatgcacg acgcgttcgg cattaccggc
     7321 ggtcatattc ctaaattcgc caaaaatttc ctcgccgaaa cgggcgacat ccgcgcggct
     7381 gtgcggcagt atatggctga agtggagtcc ggcgtttatc cgggcgaaga acacagtttc
     7441 cattgaggag tgacgttgtg ttaattatcg aaaccctgcc gctgctgcgt cagcaaatcc
     7501 gccgcctgcg tatggaaggt aagcgcgtgg cgctggtgcc caccatgggt aacctgcacg
     7561 atggccatat gaagctggtc gacgaagcca aagcccgcgc cgatgtggtc gtcgtcagta
     7621 ttttcgttaa cccaatgcag tttgaccgcc cggaagatct ggcacgttat ccacggacct
     7681 tgcaagagga ctgcgaaaag ctgaacaaac gtaaagtgga tttagttttc gcgccttcgg
     7741 taaaagagat ctacccgaac ggtacagaaa cccacaccta tgttgatgtt cctggtcttt
     7801 ccaccatgct ggaaggtgcc agccgtccgg gacattttcg cggcgtttcg actattgtca
     7861 gcaagctgtt caacctggtc cagccggaca tcgcttgctt cggcgaaaag gactttcagc
     7921 aactggcgct gatccgcaaa atggttgccg atatgggctt tgatattgag attatcggcg
     7981 taccgattat gcgcgccaaa gacggtctga cgctgagttc ccgtaacggt tatctgacgg
     8041 cggaacaacg caaaattgcg cccggtctgt acaaggtttt aagttcgatt gccgacaaat
     8101 tgcaggctgg cgaacgggat ctcgatgaaa ttattgccat tgccgggcaa gaactgaatg
     8161 aaaaaggctt ccgcgccgat gatattcaga ttcgtgatgc cgacacattg ctggaagtct
     8221 ctgaaaacag caaacgcgca gtgattctgg tagccgcttg gcttggcgat gctcgcctga
     8281 tcgacaacaa aatggtcgaa ctggcgtaat gcttaactgg cgctacggct gatggcgcca
     8341 gctattcatt gccacgttat ctgtaagcga ttattcataa cgcagactag ttagttagag
     8401 ttaattagct tatctatctc tgcaagaggt aaatttgcca actctgcaac gtcttcccga
     8461 gacattcctt tactcagaag acgctgggcc agtcttacct tctggagtcc ctctttatat
     8521 cccttctgaa atcccttctc ttcaaaccac tgcgccaacg tctgcctggt ttctctccgt
     8581 gtctttattt ccatatgttc ccttaattat ctggtcagtt aacaataaca attatctggc
     8641 tataactgat aatgcagata acttagctaa tcagcttatc aacctcagca agaggtaaat
     8701 ttgccatctc tgcaacgtct tcccgagaca tccctttact cagaaaacgc agggcgaatt
     8761 cctgtcttac ttcctgtcta acctcttgta ttacctcctg tcttacctcc tgtcttacct
     8821 cctgcctttc ctcctgtctt cccctctctt caaaccactg cgccagcgtc atcatagact
     8881 cccctcccgt ttccctgtct cgcaacacac cataaaacaa atccgcttgt tcagtatgac
     8941 cgcgttgcag catatagttt tgcatggcaa ctaactgact tccgctagtg tacccttcgt
     9001 caatcagcgt gaccagttgc tccagcaata acattaagtc gcgctggcga atatgctttt
     9061 gcagtagttc gagaatcgcg atccgccggt gctgcatgat ttcgtcatcc ggcgtgatgg
     9121 tgatatccac cagcgggaaa gggttgttgt aaacgcgtcg cgccagctcc ggcgagtaaa
     9181 acatatcaaa ccagcacatt gagagcggat aaggtgtggc ctcgccctga taaaacagaa
     9241 tcggcaccac cagcggcagc ttatcgtgat ccgcctccag atgccggtgc atggcggcaa
     9301 tggcatagcg catcatgcga aaggtcattt tcttatccgg cttgctttga tgttcaatca
     9361 ccacatgcaa ataaccggga ttaccctgca tttgcacgga atagagcacg tcagtgctgt
     9421 gccctttcag gctttcttca ataaaactcc ccgactcaag atgcagagtg tcgaggtcac
     9481 acagttcgcg tagctccgct ggcaaatgga tatccagaaa gtcgcgagcc gtttccgcat
     9541 gcattaaaaa ttgtttaaac accgcatcat gcggtgtggt actcggtgca tccatctccc
     9601 ctgccttccg tgttgtcttg cgtgaaggca gtatccagtc aaggtgtgcc gtcgtcagcc
     9661 gcctctttag tgtttaccga gcagcgttca gataaatttc aattggttac aggcgttaca
     9721 tggcacgctt cggcacggta cgcaaactgc tgatgcaaat gccgtcagat gagtagacac
     9781 taaacaaaaa tcgggcaata ctgcgtgaga atttcctcaa agcaggcatc gcctgcttcg
     9841 ttaacgacag ggtagaaagg tagaagttat gattcgcacg atgctgcagg gcaaactcca
     9901 ccgcgtgaaa gtgactcatg cggacctgca ctatgaaggt tcttgcgcca ttgatcagga
     9961 ttttcttgac gcagccggta ttctcgaaaa cgaagccatt gatatctgga atgttaccaa
    10021 cggcaagcgt ttctccacct atgccatcgc ggcagaacgc ggttcgagaa ttatttctgt
    10081 caacggtgcg gcggcccact gcgccagcgt cggcgatatt gtcatcatcg ccagcttcgt
    10141 taccatgcca gatgaagaag ctcgcacctg gcgacccaac gtcgcctatt ttgaaggcga
    10201 caacgaaatg aaacgtaccg cgaaagcgat tccggtacag gttgcctgat tgttatccct
    10261 gcggctggtt actcaccagc cgcgacatcg tctccagcga atctgttctt aagatataaa
    10321 gtcgttttag taacaacgga ttatcccccg gttttacttt gcctttcatg gtcgtcaccg
    10381 ccaggtgaaa tccggcttct tttgctgcct tcacggcttt gtcattaaat ccgccaaatg
    10441 gataagaaag atacaaaaca tgtggattaa attgcgccag agcgcggcgt gaacgtgcaa
    10501 aatcaaacag aatattgtgc tcactacggc tcagcaatat aggccgccga taaccatcta
    10561 tccgatgcaa aaaatgagta tgtgactgga aatcaaatac atcgcgaatt tcgttaagtt
    10621 cagaaacgct cataaattgc agcgattttg ggttccattt ctgcgggtga cgtttgatgc
    10681 gcgaggtaac aataaacgcc gtcgccttca tgccatattg tttcaacaca ggatacgcat
    10741 agcggctcac cgacttgagg ccatcatcaa aggtaatcac caccgctcgc gcagggagat
    10801 tgatcttatt cttcacgtaa ccttccagct gcgccatgct cagtgtcgcg tatcccctgt
    10861 cgcgcagcca ggccatctgg ttattgaaag cgcgtaccga ggtggtcgtc gaagtatggc
    10921 gaaaacgggt gttttcttca tcgcgcaaaa tatggtgata ggttagcacc ggcaggccat
    10981 tatcgggttg ggcatccagc gcgctgatat aggccagtcg atcgccaata cggatctgat
    11041 accaggtttg atttaacctg tctttcagtt tatgcaaaat cgggtagcgc aaattgtccg
    11101 ccagtactcc aaatggcgca ctgcccacac tcggcgcgtt atagaccggc gtatctttcc
    11161 aggtaactaa gttctgatta ctcagcggct tgttgaggtc acccaaaccg tcttcaactt
    11221 tttgtcgccc ctgaaccggc tcgagatggc ctttatcgat aaaccctttg ccaaagccaa
    11281 aattaaatac gtaataactt gcggcagtgg gctccacggc aatgatttgt ccggcccgaa
    11341 tattccccac cgtcaccatt ttgtcgccaa tttgcgccca gaccgcggca ttttcgatgg
    11401 tttgcatata acgggcaggt aacgcggcac tgacacttgc ggtaaacagc atcagcagga
    11461 gaataacagc ttgtttgtac atgaaaaaat aaccgagggt gaaacaacgc ttttattgta
    11521 gcaaaagcgt catcagaatc gcgggttaat actcatacaa attatgtttg aggacgaaag
    11581 gcggattttt ttgttgctgg tgccaaagac tactcacctc cggcgcaccc agttcgacaa
    11641 tacgctcgcg aaacgctgaa ctggtcatgg tttcacggca agccatcatc tgttcaatta
    11701 acggtaacgt gacaccagaa atcacttcgc aacgggagtg tttgtggctt aataatgaag
    11761 ccacgcggta cggtggcgct ccggctatat cggttaagaa aataacgcct tcaccagagt
    11821 ccgtttcatg caaggcatcg cacatcatgc ggctgagcat attagagctt aatccgcgcc
    11881 agaaattcac ggcccggcac tgaagaagtg ccccatgttt tttctccagc gcatccagta
    11941 tctcttgcgc ccggtcatcg tgacaggtaa ttacccaacc taacatcgcc ttcctcctta
    12001 gccgaaaatc agtctaccct gaccattgtt gataaagggt gatagccgtc aaaactcctc
    12061 tcccccaaat ccagaggaga ggaaatagcc ttagctacgc aaaccacgtc cacgttggat
    12121 cagcgaccaa cagatcaaat aaaacgccac aataaagacc accagtacgc caaaggtagt
    12181 gaccagcgga acatcattga taccgaggaa gccgtagcgg aaaccactga tcatataaac
    12241 gattgggttc aggtgcgaca gcccttgcca gaacggcggc aacaaagtca gggagtaaaa
    12301 caccccgccc aaatacgtga gtggcgttaa cacaaaggtt ggcaccaggc tgatgtcatc
    12361 gaacgttttg gcaaacacgc cgttcagcaa acccgcaagg gagaatagca ccgcagtgag
    12421 caccagggtt aaggcaacga atacccacga atgcacctga aacggcacaa aaaacagtga
    12481 aattgctgtc accagcatgc caacaagcag tccgcgcgct acgccaccac caacatatcc
    12541 ggcaataatc acgtgagtcg gtacgggtgc aaccagcagc tcttcaatat tacgctggaa
    12601 cttggcacca aaaaatgatg acgcgacgtt agcgtaggcg ttggtgatca ccgacatcat
    12661 gatcagcccc ggtacgatga actgcatgta gctgaagcca tgcatatcgc caatacgcga
    12721 accaatcagg ttaccgaaga taataaagta aagggtcatg gtgatgactg gcggcaccag
    12781 cgtctgcacc cagatacgca taaagcgatg gatctctttc gcccagatgc tttttagcgc
    12841 cacccagtaa agatgcatca tgtgcgatct ccttgttttt cattaaccag tgaaacaaac
    12901 agctcttcca gacggttagc tttgttacgc atacttaata cctgaatgcc ctgctcactt
    12961 aactgcgtaa atacgctgtt gatcccctgc tcacgcagca cttcaacttc cagcgtcgcg
    13021 gtatcgacca gtcgatactg atagccatcg agcttcggta acggactttt cggtgcgaga
    13081 tcgagaataa aggtttctga tttcagcttc gccagcagcg ccttcatcga agtattttcc
    13141 accagctcac cgtgttgaat aatgccgata ttgcggcaca gcatttctgc ttcttccagg
    13201 tagtgtgtgg tgagaatgat ggtggtgcct ttgtcgttta aatccttcaa aaagccccac
    13261 attgagcggc gaagttcaat atccacgcct gcggtcggtt cgtcgagaat cagtagttta
    13321 ggttcatgca ttaacgcacg ggcaatcatt aaacggcgct tcatcccgcc agataacata
    13381 cgcgcacgtt cgttgcgttt tccccataga tcgagttgtt taagatactt ttcgctgcga
    13441 atgtacgctt ctttgcgctc cacaccgtag taccctgcct gattcaccac aatttgctgc
    13501 acggtttcaa acgggttgaa gttaaattcc tgtggcacca gacccaactg acgtttagcg
    13561 tttacgacat ctttctcgag atcgtaacca aatacgctaa cccgcccgga ggttttattt
    13621 accagagagc tgataatacc gatagtggtc gattttccgg ccccgttcgg cccgagaagc
    13681 gcataaaaat cacccgcttc gacctgcaaa tcgattccac gaagcgcctg aacgccgcct
    13741 ggataggttt ttttaagctg ttgaagttcc agtgcaatgg tcataaattt ttacttacct
    13801 tacgttctta cactttatct gtggtttaaa tcgtcccgga gttgccctat attagccaaa
    13861 cgtaattatt tggttacagg tcgttaacct ccatgaaaga catagataca ctcatcagca
    13921 acaatgcact atggtcaaaa atgctggtgg aagaggatcc cgggtttttt gagaaactgg
    13981 cacaagcgca aaaaccgcgc tttctatgga ttggatgttc cgacagtcgc gttcctgcag
    14041 aacgtttaac cggtcttgag ccgggcgaac tctttgttca ccgtaatgtt gctaacctgg
    14101 tcattcacac cgacctgaac tgcctttccg tggttcagta tgcagtggat gtactcgaag
    14161 ttgaacacat tattatctgt ggccactacg gttgcggcgg cgtacaagcc gcagttgaaa
    14221 acccggaact ggggcttatc aacaactggc tgctgcatat ccgcgatatc tggttcaaac
    14281 atagctcatt gctcggcgaa atgccgcaag agcgccgtct ggataccttg tgtgaactga
    14341 acgtcatgga acaggtgtat aacctgggcc actccaccat tatgcaatca gcgtggaaac
    14401 gcgggcagaa agttaccatt cacggctggg cctacggcat tcacgacggc ttgctgcgtg
    14461 atctggatgt cacagcgact aaccgcgaaa cccttgagca acgttaccgt cacgggatct
    14521 ccaacctcaa gctgaaacac gccaaccaca aataaaaatg ccatgccgga tgcaacacat
    14581 ccggcaactt cacacttact cgtccagcag aatcactttg ccgatatacg gcagatgacg
    14641 gtaacgctgt gcgtaatcaa tgccgtaacc caccacaaac tcatccggga tcgagaaacc
    14701 gataaattct accgggacgt tcacttcacg acgggacggt ttatccagca gcgtacaaat
    14761 cgccaacgac ttcggttcgc gcaggcttaa gatctcacgc actttcgaca gtgtattccc
    14821 cgagtcgatg atatcttcaa caatcagcac gtccttgcca cggatatctt catccagatc
    14881 tttgaggatt ttcacatcac gggtggtgga catgccgcta ccgtagctgg aggcggtcat
    14941 aaagtcgact tcatgagata cctgaacttc acggcacagg tccgccataa acataaatga
    15001 gccacgcagc agacccacca gcaccatatc gctgccgctg tctttgtaac gctcagtaat
    15061 ctgacgaccc agttcagcga tacgcgcttt aatctccgct tcggggatca tta
//
LOCUS       DAHHTG010000105        14963 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.106, whole genome shotgun sequence.
ACCESSION   DAHHTG010000105 DAHHTG010000000
VERSION     DAHHTG010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14963)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14963)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14963
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.106"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            28..393
                     /locus_tag="NBI74_003363"
     CDS             28..393
                     /locus_tag="NBI74_003363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2799 domain-containing protein"
                     /protein_id="HCD1933646.1"
                     /translation="MMKKFIAPLLALLVSGCQIDPYTHAPTLTSTDWYDVGMEDAISG
                     SAIKDDDAFSDSQADRGLYLKGYAEGQKKTCQTDFTYARGLSGKSFPASCNNVENASQ
                     LHEVWRKGADENASTIRLN"
     gene            540..1058
                     /locus_tag="NBI74_003364"
     CDS             540..1058
                     /locus_tag="NBI74_003364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiR family protein"
                     /protein_id="HCD1933647.1"
                     /translation="MRFSHRLFLLLILLLTGAPILAQEPSDVAKNVRMMVSGIVSYTR
                     WPGLSGPPKLCIFSSSRFSTALQENAATSLPYLPVIIHTQQEAIISGCNGFYFGNESP
                     TFQMELTEQYPSKALLLIAEQNTECIIGSAFCLIIHNNDVRFAVNLDALSRSGVKVNP
                     DVLMLARKKNDG"
     gene            1048..2274
                     /gene="dgcN"
                     /locus_tag="NBI74_003365"
     CDS             1048..2274
                     /gene="dgcN"
                     /locus_tag="NBI74_003365"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcN"
                     /protein_id="HCD1933648.1"
                     /translation="MMDNDNSLHKRPTFKRALRNISMTSIFITMTLIWLLLSVTSVLT
                     LKQYAQKNLALTAATMTYSLEAAVVFADGPAATETLAALGQQGQFSTAEVRDKQQNIL
                     ASWHYTHKEPGDTFSNFISHWLFPAPIIQPIRHNSETIGEVRLTARDSSISHFIWFSL
                     AVLTGCILLASGIAITLTRHLHNGLVEALKNITDVVHDVRSNRNFSRRVSEERIAEFH
                     RFALDFNSLLDEMEEWQLRLQAKNAQLLRTALHDPLTGLANRAAFRSGINTLMNNSDA
                     RKTSALLFLDGDNFKYINDTWGHATGDRVLIEIAKRLAEFGGLRHKAYRLGGDEFAMV
                     LYDVQSESEVQQICSALTQIFNLPFDLHNGHQTTMTLSIGYAMTIEHASAENLQELAD
                     HNMYQAKHQRAEKLVR"
     gene            2290..2772
                     /locus_tag="NBI74_003366"
     CDS             2290..2772
                     /locus_tag="NBI74_003366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HCD1933649.1"
                     /translation="MIKHLLAPLIFTSLILTGCQSPQGKFTPEQVAAMQSYGFTESAG
                     DWSLGLSDAILFAKNDYKLLPESQQQIQTMAAKLASTGLTHARMDGHTDNYGEDSYNE
                     VLSLKRANVVADAWAMGGQIPRSNLTTQGLGKKYPIASNKTAQGRAENRRVAVVITTP
                     "
     gene            complement(2849..3196)
                     /gene="rplS"
                     /locus_tag="NBI74_003367"
     CDS             complement(2849..3196)
                     /gene="rplS"
                     /locus_tag="NBI74_003367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012768745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HCD1933650.1"
                     /translation="MSNIIKQLEQEQMKQDVPSFRPGDTVEVKVWVVEGSKKRLQAFE
                     GVVIAIRNRGLHSAFTVRKISNGEGVERVFQTHSPVVDSISVKRRGAVRKAKLYYLRE
                     RTGKAARIKERLN"
     gene            complement(3238..4005)
                     /gene="trmD"
                     /locus_tag="NBI74_003368"
     CDS             complement(3238..4005)
                     /gene="trmD"
                     /locus_tag="NBI74_003368"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HCD1933651.1"
                     /translation="MWIGIISLFPEMFRAITDYGVTGRAVKNGLLSIQSWSPRDFTYD
                     RHRTVDDRPYGGGPGMLMMVQPLRDAIHAAKAAAGEGAKVIYLSPQGRKLDQAGVSEL
                     ATNQKLILVCGRYEGIDERVIQTEIDEEWSIGDYVLSGGELPAMTLIDSVSRFIPGVL
                     GHEASATEDSFAEGLLDCPHYTRPEVLEGMEVPPVLLSGNHAEIRRWRLKQSLGRTWL
                     RRPELLENLALTEEQARLLAEFKTEHAQQQHKHDGMA"
     gene            complement(4036..4584)
                     /gene="rimM"
                     /locus_tag="NBI74_003369"
     CDS             complement(4036..4584)
                     /gene="rimM"
                     /locus_tag="NBI74_003369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HCD1933652.1"
                     /translation="MSKQLTAQAPVDPIVLGKMGSSYGIRGWLRVFSSTEDAESIFDY
                     QPWFIQKAGQWQQVQLESWKHHNQDMIIKLKGVDDRDAANLLTNCEIVVDSSQLPQLE
                     EGDYYWKDLMGCQVVTTEGYDLGKVVDMMETGSNDVLVIKANLKDAFGIKERLVPFLD
                     GQVIKKVDLTTRSIEVDWDPGF"
     gene            complement(4603..4851)
                     /gene="rpsP"
                     /locus_tag="NBI74_003370"
     CDS             complement(4603..4851)
                     /gene="rpsP"
                     /locus_tag="NBI74_003370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000256453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HCD1933653.1"
                     /translation="MVTIRLARHGAKKRPFYQVVVADSRNARNGRFIERVGFFNPIAS
                     EKEEGTRLDLDRIAHWVGQGATISDRVAALIKEVNKAA"
     gene            complement(4988..6349)
                     /gene="ffh"
                     /locus_tag="NBI74_003371"
     CDS             complement(4988..6349)
                     /gene="ffh"
                     /locus_tag="NBI74_003371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HCD1933654.1"
                     /translation="MFDNLTDRLSRTLRNISGRGRLTEDNVKDTLREVRMALLEADVA
                     LPVVREFINRVKEKAVGHEVNKSLTPGQEFVKIVRNELVAAMGEENQTLNLAAQPPAV
                     VLMAGLQGAGKTTSVGKLGKFLREKHKKKVLVVSADVYRPAAIKQLETLAEQVGVDFF
                     PSDVGQKPVDIVNAALKEAKLKFYDVLLVDTAGRLHVDEAMMDEIKQVHASINPVETL
                     FVVDAMTGQDAANTAKAFNEALPLTGVVLTKVDGDARGGAALSIRHITGKPIKFLGVG
                     EKTEALEPFHPDRIASRILGMGDVLSLIEDIESKVDRAQAEKLASKLKKGDGFDLNDF
                     LEQLRQMKNMGGMASLMGKLPGMGQIPDNVKSQMDDKVLVRMEAIINSMTMKERAKPE
                     IIKGSRKRRIAAGCGMQVQDVNRLLKQFDDMQRMMKKMKKGGMAKMMRSMKGMMPPGF
                     PGR"
     gene            6516..7307
                     /locus_tag="NBI74_003372"
     CDS             6516..7307
                     /locus_tag="NBI74_003372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417102.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YpjD"
                     /protein_id="HCD1933655.1"
                     /translation="MPVFALLALVAYSVSLALIVPGLLQKNGGWRRMAIISAVIALVC
                     HAIALEARILPDGDSGQNLSLLNVGSLVSLMICTVMTIVASRNRGWLLLPIVYAFALI
                     NLALATFMPNEYITHLEATPGMLVHIGLSLFSYATLIIAALYALQLAWIDYQLKNKKL
                     AFNQEMPPLMSIERKMFHITQIGVVLLTLTLCTGLFYMHNLFSMENIDKAVLSIVAWF
                     VYIVLLWGHYHEGWRGRRVVWFNVAGAVILTLAYFGSRIFQQLIS"
     gene            7328..8614
                     /locus_tag="NBI74_003373"
     CDS             7328..8614
                     /locus_tag="NBI74_003373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026171.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HCD1933656.1"
                     /translation="MEHISTTTLIIILIIMVVISAYFSGSETGMMTLNRYRLRHMAKQ
                     GNRSAKRVEKLLRKPDRLISLVLIGNNLVNILASALGTIVGMRLYGDAGVAIATGVLT
                     FVVLVFAEVLPKTIAALYPEKVAYPSSFLLAPLQILMMPLVWLLNAITRMLMRMMGIK
                     TDIVVSGSLSKEELRTIVHESRSQISRRNQDMLLSVLDLEKMTVDDIMVPRSEIIGID
                     INDDWKSILRQLSHSPHGRIVLYRDSLDDAISMLRVREAWRLMSEKKEFTKETMLRAA
                     DEIYFVPEGTPLSTQLVKFQRNKKKVGLVVNEYGDIQGLVTVEDILEEIVGDFTTSMS
                     PTLAEEVTPQNDGSVIIDGTANVREINKAFNWHLPEDDARTVNGVILEALEEIPVAGT
                     RVRIGEYDIDILDVQDNMIKQVKVFPVKPLRESVAE"
     gene            complement(8668..9261)
                     /gene="grpE"
                     /locus_tag="NBI74_003374"
     CDS             complement(8668..9261)
                     /gene="grpE"
                     /locus_tag="NBI74_003374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HCD1933657.1"
                     /translation="MSSKEQKTPEGQAPEEIIMDQHEEIEAVEPEASAEQVDPRDEKI
                     ANLEAQLAEAQTRERDGILRVKAEMENLRRRTELDIEKAHKFALEKFINELLPVIDSL
                     DRALEVADKANPDMSAMVEGIELTLKSMLDVVRKFGVEVIAETNVPLDPNVHQAIAMV
                     ESDDVAPGNVLGIMQKGYTLNGRTIRAAMVTVAKAKA"
     gene            9384..10262
                     /gene="nadK"
                     /locus_tag="NBI74_003375"
     CDS             9384..10262
                     /gene="nadK"
                     /locus_tag="NBI74_003375"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) kinase"
                     /protein_id="HCD1933658.1"
                     /translation="MNNHFKCIGIVGHPRHPTALTTHEMLYRWLCTKGYEVIVEQQIA
                     HELQLKNVKTGTLAEIGQQADLAVVVGGDGNMLGAARTLARYDIKVIGINRGNLGFLT
                     DLDPDNAQQQLADVLEGHYISEKRFLLEAQVCQQDCQKRISTAINEVVLHPGKVAHMI
                     EFEVYIDEIFAFSQRSDGLIISTPTGSTAYSLSAGGPILTPSLDAITLVPMFPHTLSA
                     RPLVINSSSTIRLRFSHRRNDLEISCDSQIALPIQEGEDVLIRRCDYHLNLIHPKDYS
                     YFNTLSTKLGWSKKLF"
     gene            10348..12009
                     /gene="recN"
                     /locus_tag="NBI74_003376"
     CDS             10348..12009
                     /gene="recN"
                     /locus_tag="NBI74_003376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HCD1933659.1"
                     /translation="MLAQLTISNFAIVRELEIDFHSGMTVITGETGAGKSIAIDALGL
                     CLGGRAEADMVRTGAARADLCARFSLKDTPAALRWLEENQLEDGHECLLRRVISSDGR
                     SRGFINGTAVPLSQLRELGQLLIQIHGQHAHQLLTKPEHQKFLLDGYANETSQLQEMT
                     ARYQLWHQSCRDLAHHQQLSQERAARAELLQYQLKELNEFNPQPGEFEQIDEEYKRLA
                     NSGQLLTTSQNALALMADGEDANLQSQLYTAKQLVSELIGMDSKLSGVLDMLEEATIQ
                     IAEASDELRHYCDRLDLDPNRLFELEQRISKQISLARKHHVSPEALPQYYQSLLEEQQ
                     QLDDQADSQETLALAVTKHHQQALETARALHQQRQHYANELAQLITDSMHALSMPHGQ
                     FTIDVKFDEHHLGADGADRIEFRVTTNPGQPMQPIAKVASGGELSRIALAIQVITARK
                     METPALIFDEVDVGISGPTAAVVGKLLRQLGESTQVMCVTHLPQVAGYGHQHYFVSKE
                     TDGAMTETHMQSLDKKARLQELARLLGGSEVTRNTLANAKELLAA"
     gene            12158..12499
                     /gene="bamE"
                     /locus_tag="NBI74_003377"
     CDS             12158..12499
                     /gene="bamE"
                     /locus_tag="NBI74_003377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HCD1933660.1"
                     /translation="MRCKTLTAAAAVLLMLTAGCSTLERVVYRPDINQGNYLTANDVS
                     KIRVGMTQQQVAYALGTPLMSDPFGTNTWFYVFRQQPGHEGVTQQTLTLTFNSSGVLT
                     NIDNKPALSGN"
     gene            complement(12561..12851)
                     /locus_tag="NBI74_003378"
     CDS             complement(12561..12851)
                     /locus_tag="NBI74_003378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417108.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HCD1933661.1"
                     /translation="MPGKIAVEVAYALPEKQYLQRVTLQEGATVEEAIRASGLLELRT
                     DIDLTKNKVGIYSRPAKLSDIVHDGDRVEIYRPLIADPKELRRQRAEKSANK"
     gene            complement(12841..13278)
                     /gene="ratA"
                     /locus_tag="NBI74_003379"
     CDS             complement(12841..13278)
                     /gene="ratA"
                     /locus_tag="NBI74_003379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010376629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin RatA"
                     /protein_id="HCD1933662.1"
                     /translation="MPQISRTALVPYSAEQMYQLVNDVQSYPQFLPGCTGSRILESTP
                     GQMTAAVDVSKAGISKTFTTRNQLTSNQSILMSLVDGPFKKLIGGWKFTPLSQDACRI
                     EFHLDFEFTNKLIELAFGRVFKELAANMVQAFTVRAKEVYSAR"
     gene            13449..13931
                     /gene="smpB"
                     /locus_tag="NBI74_003380"
     CDS             13449..13931
                     /gene="smpB"
                     /locus_tag="NBI74_003380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HCD1933663.1"
                     /translation="MTKKKAHKPGSATIALNKRARHEYFIEEEFEAGLALQGWEVKSL
                     RAGKANISDSYVLLRDGEAFLFGANITPMAVASTHVVCDPTRTRKLLLNQRELDSLYG
                     RVNREGYTVVALSLYWKNAWCKVKIGVAKGKKQHDKRSDIKEREWQVDKARIMKNAHR
                     "
     gene            14146..14508
                     /gene="ssrA"
                     /locus_tag="NBI74_003381"
     tmRNA           14146..14508
                     /gene="ssrA"
                     /locus_tag="NBI74_003381"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
BASE COUNT         3828 a         3897 c         3511 g         3727 t
ORIGIN      
        1 cgctatgctt tgaaaaagga gaaagaaatg atgaaaaaat ttatcgcccc cttgttggct
       61 ttactggtta gcggatgtca gattgatcct tatactcacg cgccaacctt gaccagcacc
      121 gactggtatg atgttggtat ggaagatgcg atatcgggca gcgccattaa agatgacgat
      181 gcatttagcg attcacaggc ggatcgcggt ctatacctta aaggatatgc cgaagggcaa
      241 aagaaaactt gccagaccga ttttacttat gcccgggggc tttctggtaa aagctttcct
      301 gccagctgca ataatgttga aaatgccagc caactgcatg aagtatggcg aaaaggggct
      361 gatgaaaatg ccagcacaat acgattaaat taattacccc caaaataaat taagcatgag
      421 attttactca ttatgtttta tttctcacat tgatgacgac cgcatgaaag gatgatattt
      481 taatgagcat tctgaaaaaa tatcgatgca tttcgagcga agatggtgag gatccctgaa
      541 tgcgtttttc tcaccgactg ttcttactgc tgattctcct gctgacggga gcaccaatac
      601 tcgctcagga accttctgac gttgcgaaaa acgtgcgtat gatggtttcc ggtattgtca
      661 gttacacccg ctggcctggg ctatcagggc caccaaagtt atgtatcttt tcatcttcac
      721 gttttagtac cgccttacaa gaaaatgctg ctacatcttt accttatctg cctgtcatca
      781 ttcatactca acaagaggcg ataatttcag gctgcaatgg tttttatttt ggcaacgagt
      841 caccgacatt ccagatggaa ttaaccgaac aatatccgtc aaaggcgtta ttattaattg
      901 ccgaacaaaa taccgagtgc attattggta gcgccttttg tctgatcatc cacaataatg
      961 acgtcagatt tgccgtaaac ctggatgcct tatcgcgtag cggtgtaaag gtcaacccgg
     1021 atgtcttaat gctcgcacgg aagaaaaatg atggataacg ataattctct tcataagcgc
     1081 cccacgttta aaagagcatt acgcaacatc agcatgacca gcatatttat cactatgacg
     1141 ctgatctggc tgctgctttc cgtgacgtca gtgctaaccc tgaaacaata cgcgcaaaaa
     1201 aacctggcac tgacagcagc aacaatgact tacagtctgg aagcagctgt cgtttttgcc
     1261 gatggccctg cagcaactga aacactggca gcgctgggcc agcaaggcca attttcaact
     1321 gcagaagtgc gtgataagca gcaaaatatt ctggcatcct ggcattacac ccataaggaa
     1381 ccaggcgata ctttcagcaa tttcataagc cactggctct tccctgcccc catcattcag
     1441 ccgattcgtc acaatagtga aaccattggc gaagtacgct taactgctcg cgacagttca
     1501 atcagccatt ttatctggtt ttcgctcgct gtcctgaccg gttgtattct gctggcatca
     1561 ggcatcgcaa ttaccctcac ccgccatttg cacaatggcc tggtggaagc gctgaaaaat
     1621 atcaccgatg tcgtacatga tgtgcgttct aaccgcaatt tttcccgacg agtttcggaa
     1681 gaacgtatcg ctgagtttca ccgcttcgct ctcgacttca acagtctgct ggatgaaatg
     1741 gaagagtggc agcttcgttt acaggccaaa aatgcgcagc ttctacgtac cgcgctacat
     1801 gacccattaa ccgggctggc taaccgcgca gcgtttcgta gcggcatcaa cacgttgatg
     1861 aacaattccg atgcccgaaa aacgtcggcg ttactatttc ttgatggcga taatttcaaa
     1921 tatatcaatg atacctgggg tcatgcgacg ggcgatagag tcttgattga aatagcaaaa
     1981 cggttagctg aatttggcgg gctgcgacat aaagcatacc gcctgggcgg cgatgaattc
     2041 gctatggtgc tctatgatgt acagtcggaa tctgaagtgc agcagatatg ctcagcactg
     2101 acacaaattt ttaatctccc gtttgatctt cataatggcc atcaaactac catgacatta
     2161 agcattggtt acgcgatgac cattgagcac gcctctgcgg aaaatttaca agagcttgcc
     2221 gatcacaata tgtatcaggc caaacaccag cgtgccgaaa agctggtgag ataacaagga
     2281 tatatcgata tgataaagca cctgctagcc cccctgattt tcacctcgct aatacttact
     2341 ggctgccagt cccctcaggg aaagtttact cctgagcaag tcgccgctat gcaatcttat
     2401 ggatttactg aatccgccgg cgactggtcg ctgggcttat cagatgccat tctgttcgca
     2461 aaaaatgact acaaattgct cccggaaagc cagcaacaaa tccaaaccat ggcagccaaa
     2521 ttggcctcga cagggctaac acatgcccgt atggatggac acaccgacaa ctatggtgaa
     2581 gacagttaca acgaagtctt atcattaaaa cgggcgaatg tcgtggccga tgcatgggct
     2641 atgggtggac aaattccgcg cagcaatctc accacacagg gtttaggaaa aaaatatccc
     2701 atagccagta acaagaccgc ccagggccgc gccgagaacc gccgcgtcgc agtggtgatt
     2761 acaacccctt aaaacacgag caaacagaca aaaaaagggc cagccaattg gccagccctt
     2821 cttaacagga tgtcgcttaa gcgaaatctt agttaagacg ctctttgata cgagcagcct
     2881 taccagtacg ctcacgcagg tagtacagtt tagctttacg aacagcacca cgacgtttga
     2941 cagaaatgct gtcaactacc ggagagtgag tctggaagac acgctcaacg ccttcgccgt
     3001 tggaaatttt acgaacagtg aatgcagagt gcagaccgcg gttacgaatt gcgataacca
     3061 cgccctcgaa tgcctgcaga cgttttttgg aaccttcaac aacccatact ttcacttcca
     3121 cggtatcacc cggacggaag gaaggtacgt cctgcttcat ctgctcttgt tcaagttgct
     3181 taataatgtt gctcataatt taatctctta tcctgggtaa actgatatct cgggggctta
     3241 cgccatccca tcatgtttat gttgctgttg tgcgtgttcc gttttgaact ccgccagcaa
     3301 ccttgcttgc tcttcagtca gagccaggtt ttccagaagt tcaggtcttc taagccaggt
     3361 acggcccagc gactgtttca aacgccagcg acgtatctcg gcatggttgc ccgacagtaa
     3421 cactggcgga acttccatcc cttctaacac ctcaggccgc gtatagtgcg ggcaatccag
     3481 caatccttca gcaaaggagt cttccgttgc cgaggcctca tgtcccagta ctcccggaat
     3541 aaaccgggaa acggagtcaa tcagcgtcat tgctggtaac tcaccaccac tgagaacgta
     3601 atcgccgatt gaccattctt catcaatttc ggtttggatc acgcgctcat ctataccttc
     3661 gtagcgaccg cacaccagaa tcaatttttg attcgttgcc agctcgctga cgcccgcttg
     3721 atcaagcttg cgtccctgtg gtgacagata aatcaccttt gcgccttcac ccgccgcggc
     3781 ttttgctgca tgaatggcgt cccgcaaggg ttgcaccatc attaacatcc ccggtccgcc
     3841 gccgtaagga cgatcgtcca cggtacggtg ccgatcatac gtgaagtcgc gaggactcca
     3901 gctctggatg ctcagcaggc catttttaac tgcccggcca gttaccccgt aatcggtaat
     3961 tgcgcggaac atttcaggaa acaggctaat tatgccaatc cacatagcgc cgtcttttac
     4021 cgtttatccg gtggtttaaa aaccaggatc ccaatctact tcgattgaac gagtagtgag
     4081 atcgactttc ttgataacct gcccatcgag gaacggtacg agacgttcct tgataccaaa
     4141 cgcatctttc aggtttgcct taatgacgag aacgtcatta gatccggttt ccatcatatc
     4201 tacgacttta ccgagatcgt agccttcagt ggttactacc tggcagccca tcaggtcttt
     4261 ccagtagtag tcaccctctt caagctgagg caactgcgat gaatccacga caatttcaca
     4321 attcgtcagc aggttcgccg catcacgatc gtcaacgcct ttcagcttga tgatcatgtc
     4381 ctgattgtgg tgcttccagc tttccagctg gacttgctgc cactgacccg ccttctggat
     4441 aaaccagggc tgatagtcaa aaatgctttc ggcgtcttcg gtggaagaaa acactctgag
     4501 ccacccacga ataccgtaag acgaacccat ttttcccaaa acgatgggat caacaggtgc
     4561 ttgcgcggtg agttgtttgc tcatcatgac caccgtgaca gattaagctg ctttgtttac
     4621 ttctttgatc agcgcagcaa cgcgatcaga aatagttgcg ccctggccaa cccagtgagc
     4681 gatgcgatcc agatccaggc gagtgccttc ttctttttcg ctagcgattg ggttgaagaa
     4741 accaacgcgc tcgatgaagc gaccgttgcg tgcattacgg ctgtcagcga caacaacctg
     4801 gtagaacgga cgctttttag cgccgtgacg tgctaaacga atagttacca taacatcctc
     4861 ttgtgtgaat aaaacaaccg gaccccatcg aggaacggag tccggtgtca tattaaaagc
     4921 ccgaaaattt tactgatttc tggagaaaat gcaatcagca gttaataact ctgtcgcata
     4981 aggtcattta gcgaccagga aatcccggcg gcatcatccc tttcatgctt ctcatcatct
     5041 tcgccatccc gcccttcttc attttcttca tcatgcgctg catgtcgtcg aactgtttca
     5101 gaagacggtt aacgtcctgc acctgcatac cgcaaccggc agcgatacgg cgcttacgcg
     5161 aacctttgat gatttctggc ttagcgcgct ctttcatcgt catcgagttg atgatggctt
     5221 ccatacgcac cagcacttta tcgtccatct gcgacttgac gttatccggg atctgcccca
     5281 tgcccggcag cttgcccatc aggctagcca tgccgcccat atttttcatc tggcgcagct
     5341 gctcaagaaa gtcgttgaga tcgaagccgt cgcctttttt cagcttgctg gctaatttct
     5401 cagcttgcgc gcggtcaact ttgctttcga tatcttcgat cagtgacagt acgtcgccca
     5461 tgccgagaat acgggaggca atacggtccg gatggaatgg ctccagcgcc tcggttttct
     5521 cgccaacacc gaggaacttg atcggtttgc cagtgatgtg acgaatagag agcgccgcac
     5581 cgccgcgggc atcgccgtcc actttggtca acactacacc ggtaagcggt aacgcttcat
     5641 tgaatgcttt tgccgtattg gccgcatcct gaccggtcat ggcatcgacc acaaataggg
     5701 tttcaaccgg attaatcgac gcatggactt gtttgatctc gtccatcatc gcttcgtcaa
     5761 cgtgcagacg accggcagta tccaccagca gcacgtcgta gaatttcagt ttggcttctt
     5821 tcagcgccgc gttaacgata tctaccggct tctgaccaac atcagaaggg aagaaatcaa
     5881 cgcccacctg ctctgccagc gtctcaagct gtttgattgc cgccgggcga tagacgtcgg
     5941 cagaaaccac cagtactttc ttcttgtgct tctcgcgcag gaacttaccg agtttaccaa
     6001 cgctggttgt tttaccggca ccttgcaggc ccgccatcag cacgaccgca ggcggttgcg
     6061 cagccaggtt cagggtctgg ttctcttcgc ccatcgccgc aaccagttcg ttacggacaa
     6121 ttttgacgaa ctcctgcccc ggcgtcaggc tcttattaac ttcatgacca accgctttct
     6181 cttttacgcg attgataaac tcacgcacta ccggcagagc gacgtcagcc tccagcagcg
     6241 ccatgcgcac ttcgcgcagc gtatctttta cgttgtcttc agtgaggcgt ccacggccac
     6301 tgatattgcg cagcgtgcgc gacaaacgat cggttaaatt atcaaacatt gtctctcgcc
     6361 tggggtggaa acggttggcc gcaatcgcga cacatcatca gtattttgcc gcagtataac
     6421 atgaaggcgt ctttgttgtt atgcaacggt tggagcagcg ttcacctgac gctatactgc
     6481 ttctctttct tattgctcaa actgtcgaca ttactatgcc cgtttttgct ctgctcgcgc
     6541 ttgtcgccta ctccgtcagt cttgcgctga ttgttcccgg tctgctgcaa aaaaacggcg
     6601 gctggcggcg catggctatt atttctgcgg tcatcgcgct ggtctgccac gcaatcgctc
     6661 tggaagcccg catcctgccc gacggtgata gcggacaaaa cctcagcctg ctgaacgttg
     6721 gttcattggt cagtttgatg atctgtacgg taatgaccat tgtggcttct cgcaatcgtg
     6781 gctggttgtt gctacccatt gtctatgcct ttgcgcttat caacctggcg ctggcgacct
     6841 ttatgcccaa tgaatacatc acccatctgg aagcaacgcc ggggatgctg gtgcacattg
     6901 gcttatcgct tttttcctat gccacgctaa ttatcgctgc cctgtacgcg ctgcaactgg
     6961 cgtggattga ttaccaactg aagaacaaga agctggcgtt taaccaggaa atgccgccat
     7021 tgatgagtat cgagcgtaaa atgttccata tcacgcagat tggcgtggtg ctgctaacgc
     7081 tcactctttg cactggcctg ttctacatgc acaacttatt tagcatggaa aatatcgaca
     7141 aggccgtgct ctctatcgtg gcgtggtttg tctatattgt gctgctgtgg ggacattatc
     7201 atgaaggatg gcgtggacgc cgcgtcgtct ggtttaacgt tgcgggtgca gtcattctga
     7261 cactggccta cttcggcagc cgaatttttc agcagttaat cagctaaacc cagaaaagga
     7321 gtttcccctg gaacacattt ctactactac gttgatcatt attctgatca tcatggtggt
     7381 catttcagcc tatttttccg ggtccgaaac cggaatgatg accctcaacc gctatcgtct
     7441 gcggcatatg gcgaaacagg gtaatcgctc ggccaaacgc gtcgaaaaat tgctgcgtaa
     7501 gccagaccgc ctgataagcc tggtgttaat cggcaataac ctggtcaata ttcttgcctc
     7561 cgcgctcggc actattgttg gaatgcgttt gtacggcgat gcgggcgtgg caattgcgac
     7621 tggtgtgctg acttttgtcg tgctggtttt tgctgaggta ttgccaaaaa ccattgccgc
     7681 gctgtacccg gaaaaagtcg cttatccgag tagttttctg ctggctccgc tgcaaatttt
     7741 gatgatgccg ctggtctggt tgctgaatgc tattacccgc atgttaatgc gcatgatggg
     7801 catcaaaacc gatatcgtgg ttagtggctc tttaagcaaa gaagaattac gcaccattgt
     7861 gcacgaatcg cggtcacaaa tttcccgtcg caatcaggat atgctgctgt cggtactcga
     7921 tctggaaaag atgaccgtcg atgacatcat ggtgccgcgc agtgaaatta tcggtattga
     7981 tatcaacgat gactggaaat cgattctgcg ccaactctcc cactcacctc acgggcgcat
     8041 cgtgctctac cgtgattcgc tggacgacgc catcagtatg ctgcgtgtgc gtgaagcctg
     8101 gcgactcatg tcggagaaaa aagagttcac caaagaaacc atgctacgcg ccgcggacga
     8161 gatctatttt gtcccggagg gtacgccgct cagcacacag ttggtgaagt ttcagcgcaa
     8221 caaaaagaaa gtcggcctgg tcgtcaacga gtatggagac attcaggggc tggtgacggt
     8281 cgaagatatt ctcgaagaga ttgtcggcga tttcaccacg tcgatgtcgc caacacttgc
     8341 cgaagaggtc acgccgcaaa acgacgggtc ggtgattatc gatggcaccg ccaacgtgcg
     8401 agaaattaac aaagccttta actggcatct accggaagat gatgctcgta ctgttaacgg
     8461 cgtcattctg gaagcgctgg aggagattcc ggtagcaggc acccgcgtgc gtataggcga
     8521 gtacgatatc gatattctcg acgtacagga caatatgatt aagcaggtaa aagtttttcc
     8581 tgtaaaaccg ctgcgcgaga gtgtggcgga gtaaaaaaac ggcccggcat tcgcatgctg
     8641 gaccgtgatt tattacgaaa acagaaatca agcttttgcc ttcgctacag tcaccatcgc
     8701 cgcacgaatt gtacgaccgt tcagcgtata acccttctgc ataatgccca gtacgttacc
     8761 tggcgcaacg tcatcagatt ccaccattgc gatggcctga tgcacattcg ggtccagtgg
     8821 tacgttagtt tcggcgatca cttcaacgcc aaacttacgc acaacatcca gcatcgactt
     8881 cagcgtcagc tcaatacctt caaccatcgc ggacatatcc gggttagctt tatcagccac
     8941 ttccagcgca cgatccaggc tatcaatcac cggcagcaat tcgttgatga atttctccag
     9001 cgcgaatttg tgagcttttt caatatccag ttcagtacga cgacgcaggt tttccatttc
     9061 ggcttttacg cgcaaaatgc cgtcacgttc acgggtctgg gcttcagcca gctgagcttc
     9121 gagattcgca attttttcat cgcgcggatc cacctgctca gcagaagctt ctggctcaac
     9181 tgcctcaatc tcttcgtgct gatccatgat aatttcttcc ggggcttgcc cctcaggcgt
     9241 tttctgttct ttactactca tgaatttctc cgcgtttttt tcgcattcat ctcgctaact
     9301 tcgcttatta tggggatcag tttcagggtt tcaagggaag cactcacatt gtcatcaatc
     9361 ttcgcaacaa ggacctcgga aaaatgaata atcatttcaa gtgtattggc attgtgggac
     9421 acccacggca ccccactgca ctgacaacac atgaaatgct ctaccgctgg ctgtgcacaa
     9481 aaggttacga ggtcatcgtt gagcaacaaa tcgctcacga actgcaactg aagaatgtga
     9541 aaactggcac gctcgcggag attgggcaac aggctgatct cgcggttgtc gttggcggcg
     9601 acggtaatat gctgggcgcg gcgcgcacgc tcgcccgcta cgatattaaa gttattggaa
     9661 tcaaccgtgg caacctgggt ttcctgactg accttgatcc cgataacgcc cagcaacaat
     9721 tagccgatgt gctggaaggc cactacatca gcgagaaacg ttttttgctg gaagcgcaag
     9781 tctgtcagca agattgccag aaacgcatca gcacggcgat taacgaagtg gtacttcatc
     9841 ctggcaaagt agcgcatatg attgagttcg aagtgtatat cgacgagatc tttgcgtttt
     9901 cgcagcgttc cgatggactg attatttcga cgccaacagg ctccaccgca tattccctct
     9961 ctgcgggcgg tcctattctg acaccctctc tggatgcgat taccctggtg cccatgttcc
    10021 cgcatacgtt gtcagcacga ccactggtca taaacagcag cagcacgatc cgtctgcgtt
    10081 tttcgcatcg ccgtaacgac ctggaaatca gttgcgacag ccagatagca cttccgattc
    10141 aggaaggtga agatgtcctg attcgtcgct gtgattacca tctgaatctg attcatccga
    10201 aagattacag ttatttcaat acattaagca ccaagctcgg ctggtcaaaa aaattattct
    10261 aattttacgc cagcctcttt actgtatata aaaccagttt atactgtaca caataacagt
    10321 aatggttttt catacaggaa aacaactatg ttggcacaac tgaccatcag caactttgct
    10381 atcgttcgtg agcttgagat tgattttcat agcggcatga ccgtaataac tggcgagacc
    10441 ggcgcgggta aatctattgc aatagatgcc ctcggccttt gtctcggtgg tcgcgctgaa
    10501 gccgacatgg tgcgtaccgg cgctgctcgt gccgacctgt gcgcccgttt ttctctgaaa
    10561 gatacgccag cggccctgcg ttggctggaa gaaaaccagc ttgaagacgg gcatgaatgt
    10621 ttgcttcgtc gcgtaatcag cagcgatggt cgctcccgtg gtttcatcaa cggtacagct
    10681 gttcctctgt cacaactgcg cgaactgggt cagttgctga ttcagatcca tggtcagcac
    10741 gctcatcagt tactcaccaa acccgagcac caaaaattcc tgcttgatgg ctatgccaat
    10801 gaaacctctc agctccagga aatgaccgca cgttatcagt tgtggcatca aagctgccgt
    10861 gacctcgcgc atcatcagca gttaagtcag gaacgcgccg cccgtgcgga gctgctgcaa
    10921 taccaattaa aagaacttaa cgaatttaat ccgcagcctg gagagtttga gcaaatcgac
    10981 gaagagtaca aacgtctggc gaacagcggt caattgctga ccaccagcca gaatgcattg
    11041 gcattaatgg ctgacggtga agacgcaaat ctgcaaagtc aactttacac ggctaaacaa
    11101 ctggtgagcg aattgattgg catggacagc aaactgtccg gcgtacttga tatgctggaa
    11161 gaggctacca tccagattgc tgaagccagt gatgaactac gccactactg cgatcgtctg
    11221 gatctcgatc ccaaccgact gtttgaactt gaacagcgca tctcaaaaca gatttcgctg
    11281 gcacgtaaac atcacgtcag ccctgaggca ttgccacagt attaccagtc gctactggaa
    11341 gaacagcagc aactggacga tcaggccgac tcacaagaaa cgctcgcgct ggcggtaacg
    11401 aaacatcatc agcaggcact ggaaacggcg cgcgcgttac accagcaacg ccagcattat
    11461 gcaaatgagt tggcacagct gatcaccgat agtatgcatg cactctctat gccacatggg
    11521 cagtttacga tcgatgttaa atttgacgag catcacctgg gcgctgacgg cgctgatcgt
    11581 attgagtttc gggtaaccac caacccaggt cagccaatgc agcctattgc caaagtcgca
    11641 tccggtggtg agttgtcccg catcgcattg gcaatccagg tcattaccgc gcgtaaaatg
    11701 gaaaccccag cactgatttt tgatgaagtg gatgtaggga ttagcggccc aacagcggca
    11761 gttgtcggca aactgctacg tcaacttggc gaatcaactc aggtgatgtg tgttacccac
    11821 ctgccacaag tcgcgggata tggtcatcaa cactattttg tcagcaaaga aaccgatggt
    11881 gcgatgacag aaacgcatat gcaatccctg gataaaaaag cgcggttaca agagttggcg
    11941 cgcctgcttg gtggcagtga agtcacacgt aacacactgg cgaatgcgaa agaactgctt
    12001 gcggcgtaaa cttttttcct gcttcacggt cagagtaaac agcaaaacgc cgtaagaccg
    12061 gaaagcaaaa ggttttaaag tgatgaaagg tctattatca tcggcatatt acagatgagc
    12121 cacgtactgc tcgggcccga aaaggaatca aatcactatg cgctgtaaaa cgctgactgc
    12181 tgcagcagca gtactattga tgttgaccgc aggctgttcc actctggagc gagtggttta
    12241 ccgtcctgac atcaaccagg ggaactatct gaccgctaac gacgtatcca aaatacgtgt
    12301 tggcatgacg caacaacaag ttgcgtacgc attgggtaca ccgctgatgt ccgatccatt
    12361 tggtacaaat acctggttct atgtcttccg ccagcaacct ggtcatgaag gtgtaacgca
    12421 gcaaacgctg acgctgacct ttaacagtag cggtgtgttg accaacattg ataacaaacc
    12481 tgcgctgagt ggtaactaat aataaagttg ctctcaaaga cgttaaaaaa ggtgctcaat
    12541 gagcaccttt tttctgtctg ttatttattc gctgattttt ctgctcgttg cctgcgaagc
    12601 tctttcggat cggcaatgag aggacgataa atctccaccc gatcgccatc atgcacaata
    12661 tcgctgagtt ttgccggacg gctgtaaatg ccgactttgt ttttagttaa atcgatatcg
    12721 gtacgcaatt ccagcaaacc actggcgcga atagcttctt caaccgtcgc gccctcctgc
    12781 aacgtcacgc gctgcaggta ctgcttctca ggtagcgcat aagccacctc aacggcaatt
    12841 ttacctggca ctgtagacct cttttgcacg aacagtaaaa gcctgcacca tattagctgc
    12901 cagctcttta aacacacgac caaaggcgag ttcaatcaac ttattggtaa actcaaagtc
    12961 gagatgaaac tcaatacggc acgcgtcctg gctcaacggc gtaaacttcc aaccgcctat
    13021 caatttctta aacggtccat caaccagact catgagaata ctttgattac tggtcaactg
    13081 gttgcgggtt gtaaacgttt tgctgatccc agccttagac acatctaccg cagcggtcat
    13141 ctgcccagga gtggactcca gaatccggct tccagtgcac cctggtaaaa actgaggata
    13201 agactgaacg tcattcacta actgatacat ttgctccgcg ctgtagggta ccagtgcggt
    13261 ccggctaatc tgaggcataa caatttccat caacaaaaat ccaacaaata atatcattta
    13321 tcctgctgta aaaaaaacgc tatcccggcg ctgggtaaca tcgggatcat gctaagatag
    13381 agccttgtcc cccgcaggat tgatatgggg tgttttcgat ttcagattac cgatgattca
    13441 cgacgcttat gacgaagaaa aaagcacata aacctggttc agcgaccatc gcgcttaaca
    13501 agcgcgcccg tcacgaatac tttatcgaag aagagttcga agcgggactt gccctgcaag
    13561 gctgggaggt taaatccctg cgtgcaggaa aagccaatat cagcgacagc tacgtccttc
    13621 tgcgtgacgg agaggcattt ctgtttggcg ctaacatcac gccaatggct gtggcctcca
    13681 cgcatgtggt gtgcgatcct acccgtaccc gcaagttact tctcaaccag cgcgaactgg
    13741 actcattgta cggtcgcgta aatcgagaag gctataccgt agtggcgctc tccctgtact
    13801 ggaaaaatgc ctggtgcaaa gtgaaaatcg gcgtcgccaa aggtaaaaaa cagcacgata
    13861 aacgttcaga tatcaaagaa cgcgaatggc aggtggataa agcgcgtatc atgaaaaacg
    13921 cccatcgtta aacctatata agcgatacta atcaaatcct caccgcgcct ccctatccgg
    13981 tggcgcgaat gaacatcttg tcgactgtca aatctgacac agatgtagcc atcccattgc
    14041 ttaatcgaaa aaaaatcata ctatgcaggc gctaattcca ggatgataac gccttttggg
    14101 ctagtcatgc tgctcataaa tctggtatac ttaccgttac acattggggc tgattctgga
    14161 ttcgacggga tttgcgaaac ccaaggtgca tgccgagggg cggttggcct cgtaaaaagc
    14221 cgcaaaaaat agtcgcaaac gacgaaaact acgctttagc agcttaataa cctgcttaga
    14281 gccctctctc cctagcctcc gctcttagga cggggatcaa gagaggtcaa acccaaaaga
    14341 gatcgcgtgg aagccctgcc tggggttgaa gcgttaaaac ttaatcaggc tagtttgtta
    14401 gtggcgtgtc cgtccgcagc tggcaagcga atgtaaagac tgactaagca tgtagtaccg
    14461 aggatgtagg aatttcggac gcgggttcaa ctcccgccag ctccaccaaa taatgattgg
    14521 acggtgtaag gactacacca acaaaaacag gaagttataa gtctcagcag gacaccgacc
    14581 agacggtgag gagacaaaaa aggatacgca acggagtcgc gactccctat gacattaaag
    14641 cccgctgatg cgggcttttt tattggtcct gacccgccct gaatagaatt catccaatta
    14701 cagtccagac atatacagta tccctcagct cagtatcagc ttctcgacaa atggtcactg
    14761 ttatttaccc gtttccagaa agagaataat ttgcccccat agccagcctt cccggaatct
    14821 gtatgttcat taatattgtt atcgatgcca tcaagtaaca taggttggat gcgattgatc
    14881 ttactcacat tagtcgacac ggaactcagt aagttgaact ctgaaaaagc agtttaaata
    14941 gcatttaaac aaatgccacc agt
//
LOCUS       DAHHTG010000106        14952 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.107, whole genome shotgun sequence.
ACCESSION   DAHHTG010000106 DAHHTG010000000
VERSION     DAHHTG010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14952)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14952)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14952
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.107"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            88..1197
                     /gene="anmK"
                     /locus_tag="NBI74_003382"
     CDS             88..1197
                     /gene="anmK"
                     /locus_tag="NBI74_003382"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HCD1933664.1"
                     /translation="MKSGRFIGVMSGTSLDGVDVVLATIDEHRVAQLASLSWPIPVSL
                     KQAVLDICQGQQLTLSQFGQLDTQLGRLFADAVNALLKEQNLQARDIVAIGCHGQTVW
                     HEPTGVAPHTLQIGDNNQIVARTGITVVGDFRRRDIALGGQGAPLVPAFHHALLAHPT
                     ERRMVLNIGGIANLSLLIPGQPVGGYDTGPGNMLMDAWIWRQAGKPYDKDAEWARAGK
                     VILPLLQNMLSDPYFSQPAPKSTGREYFNYGWLERHLRHFPGVDPRDVQATVAELTAV
                     TISEQVLLSGGCERLMVCGGGSRNPLLMARLAALLPGTEVTTTDAVGISGDDMEALAF
                     AWLAWRTLAGLPGNLPSVTGASQETVLGAIFPANP"
     gene            1295..1624
                     /gene="mliC"
                     /locus_tag="NBI74_003383"
     CDS             1295..1624
                     /gene="mliC"
                     /locus_tag="NBI74_003383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416156.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-type lysozyme inhibitor"
                     /protein_id="HCD1933665.1"
                     /translation="MSMKKLLLICLPVLLTGCSAFNQLVERMQTDTLEYQCDEKPLTV
                     KLNNPRQEVSFVYDNQLLHLKQGISASGARYTDGIYVFWSKGEEATVYKRDRIVLNNC
                     QLQNPQR"
     gene            1683..2339
                     /gene="pdxH"
                     /locus_tag="NBI74_003384"
     CDS             1683..2339
                     /gene="pdxH"
                     /locus_tag="NBI74_003384"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HCD1933666.1"
                     /translation="MSDNDELQQIAHLRREYTKGGLRRRDLPADPLTLFERWLSQACE
                     AKLADPTAMVVATVDEHGQPYQRIVLLKHYDEKGMVFYTNLGSRKAHQIENNPRVSLL
                     FPWHTLERQVMVIGKAERLSTLEVMKYFHSRPRDSQIGAWVSKQSSRISARGILESKF
                     LELKQKFQQGEVPLPSFWGGFRVSLEQIEFWQGGEHRLHDRFLYQRENDAWKIDRLAP
                     "
     gene            2468..3742
                     /gene="tyrS"
                     /locus_tag="NBI74_003385"
     CDS             2468..3742
                     /gene="tyrS"
                     /locus_tag="NBI74_003385"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HCD1933667.1"
                     /translation="MASSNLIKQLQERGLVAQVTDEEALAERLAQGPIALYCGFDPTA
                     DSLHLGHLVPLLCLKRFQQAGHKPVALVGGATGLIGDPSFKAAERKLNTEETVQEWVD
                     KIRKQVAPFLDFDCGENSAIAANNYDWFGNMNVLTFLRDIGKHFSVNQMINKEAVKQR
                     LNREDQGISFTEFSYNLLQGYDFACLNKQYGVVLQIGGSDQWGNITSGIDLTRRLHQN
                     QVFGLTVPLITKADGTKFGKTEGGAVWLDPKKTSPYKFYQFWINTADADVYRFLKFFT
                     FMSIEEINALEEEDKNSGKAPRAQYVLAEQVTRLVHGEEGLQAAKRITECLFSGSLSA
                     LSEADFEQLAQDGVPMVEMEKGADLMQALVDSELQPSRGQARKTIASNAITINGEKQS
                     DPEYFFKEEDRLFGRFTLLRRGKKNYCLICWK"
     gene            3804..4664
                     /gene="pdxY"
                     /locus_tag="NBI74_003386"
     CDS             3804..4664
                     /gene="pdxY"
                     /locus_tag="NBI74_003386"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310372.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal kinase PdxY"
                     /protein_id="HCD1933668.1"
                     /translation="MKNILAIQSHVVYGHAGNSAAEFPMRRLGANVWPLNTVQFSNHT
                     QYGKWTGCVMPPSHLTEIVQGIAAIDKLHTCDAVLSGYLGSAEQGEHILGIVRQVKAA
                     NPQAKYFCDPVMGHPEKGCIVAPGVAEFHVRHGLPASDIIAPNLVELEILCEHPVNNV
                     EEAVLAARELIAQGPQIVLVKHLARAGYSRDRFEMLLVTADEAWHISRPLVDFGMRQP
                     VGVGDVTSGLLLVKLLQGATLQEALEHVTAAVYEIMVTTKAMQEYELQVVAAQDRIAN
                     PEHYFSATKL"
     gene            complement(4708..5313)
                     /gene="gstA"
                     /locus_tag="NBI74_003387"
     CDS             complement(4708..5313)
                     /gene="gstA"
                     /locus_tag="NBI74_003387"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase GstA"
                     /protein_id="HCD1933669.1"
                     /translation="MKLFYKPGACSLASHITLRESGKDFTLVSVDLMKKRLENGDDYF
                     AVNPKGQVPALLLDDGTLLTEGVAIMQYLADSVPDRQLLAPVNSISRYKTIEWLNYIA
                     TELHKGFTPLFRPDTPEEYKPTVRAQLEKKLQYVNEALKDEHWICGQRFTIADAYLFT
                     VLRWAYAVKLNLEGLEHIAAFMQRMAERPEVQDALSAEGLK"
     gene            complement(5419..6921)
                     /gene="dtpA"
                     /locus_tag="NBI74_003388"
     CDS             complement(5419..6921)
                     /gene="dtpA"
                     /locus_tag="NBI74_003388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpA"
                     /protein_id="HCD1933670.1"
                     /translation="MSTANQKPTESVSLNAFKQPKAFYLIFSIELWERFGYYGLQGIM
                     AVYLVKQLGMSEADSITLFSSFSALVYGLVAIGGWLGDKVLGTKRVIMLGAIVLAIGY
                     ALVAWSGHDAGIVYMGMAAIAVGNGLFKANPSSLLSTCYEKNDPRLDGAFTMYYMSVN
                     IGSFFSMIATPWLAAKYGWSVAFALSVVGLLITIVNFAFCQRWVKQYGSKPDFEPINY
                     RNLLLTIIGVVALIAIATWLLHNQEVARMALGVVAFGIVVIFGKEAFAMKGAARRKMI
                     VAFILMLEAIIFFVLYSQMPTSLNFFAIRNVEHTILGLAVEPEQYQALNPFWIIIGSP
                     ILAAIYNKMGDTLPMPTKFAIGMVMCSGAFLILPLGAKFASDAGIVSVSWLVASYGLQ
                     SIGELMISGLGLAMVAQLVPQRLMGFIMGSWFLTTAGANLIGGYVAGMMAVPDNVTDP
                     LMSLEVYGRVFLQIGVATAVIAVLMLFTAPKLHRMTQDDAADKAAKAAVA"
     gene            complement(7532..8167)
                     /gene="nth"
                     /locus_tag="NBI74_003389"
     CDS             complement(7532..8167)
                     /gene="nth"
                     /locus_tag="NBI74_003389"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HCD1933671.1"
                     /translation="MNKAKRLEILTRLRENNPHPTTELNFSSPFELLIAVLLSAQATD
                     VSVNKATAKLYPVANTPAAMLELGVEGVKTYIKTIGLYNSKAENIIKTCRILLEQHNG
                     EVPEDRAALEALPGVGRKTANVVLNTAYGWPTIAVDTHIFRVCNRTQFAPGKNVEQVE
                     EKLLKVVPAEFKVDCHHWLILHGRYTCIARKPRCGSCIIEDLCEYKEKVDI"
     gene            complement(8167..8862)
                     /gene="rsxE"
                     /locus_tag="NBI74_003390"
     CDS             complement(8167..8862)
                     /gene="rsxE"
                     /locus_tag="NBI74_003390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxE"
                     /protein_id="HCD1933672.1"
                     /translation="MSEIKDVIVQGLWKNNSALVQLLGLCPLLAVTSTATNALGLGLA
                     TTLVLTLTNLTISTLRHWTPAEIRIPIYVMIIASVVSAVQMLINAYAFGLYQSLGIFI
                     PLIVTNCIVVGRAEAFAAKKGPALSALDGFSIGMGATCAMFVLGSLREIIGNGTLFDG
                     ADALLGSWAKVLRVEIFHTDSPFLLAMLPPGAFIGLGLMLAGKYLIDEKMKKRRTEAA
                     AERALPNGETGNV"
     gene            complement(8866..9486)
                     /gene="rsxG"
                     /locus_tag="NBI74_003391"
     CDS             complement(8866..9486)
                     /gene="rsxG"
                     /locus_tag="NBI74_003391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxG"
                     /protein_id="HCD1933673.1"
                     /translation="MLKTIRKHGITLALFAAGSTGLTAAINQMTKTTIAEQASLQQKA
                     LFDQVLPAERYNNALAQSCYLVTAPELGKGEHRVYIAKQDDKPVAAVLEATAPDGYSG
                     AIQLLVGADFNGTVLGTRVTEHHETPGLGDKIELRLSDWITHFAGKKISGADDANWAV
                     KKDGGDFDQFTGATITPRAVVNAVKRAGLYAQTLPAQLSQLPACGE"
     gene            complement(9490..10548)
                     /gene="rsxD"
                     /locus_tag="NBI74_003392"
     CDS             complement(9490..10548)
                     /gene="rsxD"
                     /locus_tag="NBI74_003392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxD"
                     /protein_id="HCD1933674.1"
                     /translation="MVFRIASSPYTHNQRQTSRIMMLVLLAAVPGIAAQLWFFGWGTL
                     VQILLASVSALLAEALVLKLRKQSVAATLKDNSALLTGLLLAVSIPPLAPWWMVVLGT
                     VFAVIIAKQLYGGLGQNPFNPAMIGYVVLLISFPVQMTSWLPPHEIAVNIPGFIDAIQ
                     VIFSGHTTSGGDMNTLRLGIDGISQATPLDTFKTSVRAGHSVEEIMQYPIYSGILAGA
                     GWQWVNLAWLTGGVWLLWQKAIRWHIPLSFLVTLALCATLGWLFSPDTLAAPQIHLLS
                     GATMLGAFFILTDPVTASTTNRGRLIFGALAGLLVWLIRSFGGYPDGVAFAVLLANIT
                     VPLIDYYTRPRVYGHRKG"
     gene            complement(10549..12969)
                     /gene="rsxC"
                     /locus_tag="NBI74_003393"
     CDS             complement(10549..12969)
                     /gene="rsxC"
                     /locus_tag="NBI74_003393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000915809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HCD1933675.1"
                     /translation="MLKLFSAFRKNKIWDFNGGIHPPEMKTQSNGTPLRQVPLAQRFV
                     IPLKQHIGAEGELCVSVGDKVLRGQPLTRGRGKMLPVHAPTSGTVTAIAPHSTAHPSA
                     LAELSVIIDADGEDCWIPRDGWADYRSRSREELIERIHQFGVAGLGGAGFPTGVKLQG
                     GGDKIETLIINAAECEPYITADDRLMQDCAAQVVEGIRILAHILQPREILIGIEDNKP
                     QAISMLRAVLADSHDISLRVIPTKYPSGGAKQLTYILTGKQVPHGGRSSDIGVLMQNV
                     GTAYAVKRAVIDGEPITERVVTLTGEAIARPGNVWARLGTPVRHLLNDAGFCPSADQM
                     VIMGGPLMGFTLPWLDVPVVKITNCLLAPSANELGEPQEEQSCIRCSACADACPADLL
                     PQQLYWFSKGQQHDKATTHNIADCIECGACAWVCPSNIPLVQYFRQEKAEIAAIRQEE
                     KRAAEAKARFEARQARLEREKTARLERHKSAAVQPAAKDKDAIAAALARVKEKQAQAT
                     QPIVIKAGERPDNSAIIAAREARKAQARAKQAELQQTNDAATVADPRKTAVEAAIARA
                     KARKLEQQQANAEPEQQVDPRKAAVEAAIARAKARKLEQQQANAEPEQQVDPRKAAVE
                     AAIARAKARKLEQQQANAEPEEQVDPRKAAVEAAIARAKARKLEQQQQANAEPEEQVD
                     PRKAAVEAAIARAKARKLEQQQANAEPEQQVDPRKAAVEAAIARAKARKLEQQQQANA
                     EPEEQVDPRKAAVEAAIARAKARKLEQQQANAEPEEQIDPRKAAVAAAIARVQAKKAA
                     QQKVVNED"
     gene            complement(12962..13540)
                     /gene="rsxB"
                     /locus_tag="NBI74_003394"
     CDS             complement(12962..13540)
                     /gene="rsxB"
                     /locus_tag="NBI74_003394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxB"
                     /protein_id="HCD1933676.1"
                     /translation="MNAIWIAVAAVSLLALAFGAILGYASRRFAVEDDPVVEKIDEIL
                     PQSQCGQCGYPGCRPYAEAISCNGEKINRCAPGGEAVMLKIAELLNVEPQPLDGEAQE
                     LTPARMVAVIDENNCIGCTKCIQACPVDAIVGATRAMHTVMSDLCTGCNLCVDPCPTH
                     CISLQPVAETPDSWKWDLNTIPVRIIPVEHHA"
     gene            complement(13540..14121)
                     /gene="rsxA"
                     /locus_tag="NBI74_003395"
     CDS             complement(13540..14121)
                     /gene="rsxA"
                     /locus_tag="NBI74_003395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017456621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HCD1933677.1"
                     /translation="MTDYLLLFVGTVLVNNFVLVKFLGLCPFMGVSKKLETAMGMGLA
                     TTFVMTLASICAWLIDTWILIPLNLIYLRTLAFILVIAVVVQFTEMVVRKTSPVLYRL
                     LGIFLPLITTNCAVLGVALLNINLGHNFLQSALYGFSAAVGFSLVMVLFAAIRERLAV
                     ADVPAPFRGNAIALITAGLMSLAFMGFSGLVKL"
     gene            complement(14198..14638)
                     /locus_tag="NBI74_003396"
     CDS             complement(14198..14638)
                     /locus_tag="NBI74_003396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707526.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2569 domain-containing protein"
                     /protein_id="HCD1933678.1"
                     /translation="MTTTTPQRIGGWLLGPLAWLLVALLSTTLALLLYTAALSSPQTF
                     QTLGGQALTTQILWGVSFITAIAMWYYTLWLTIAFFKRRRCVPKHYIIWLLISVLLAV
                     KAFAFSPVEDGIAVRQLLFTLLATALIVPYFKRSSRVKATFVNP"
     gene            complement(14724..14939)
                     /gene="ydgT"
                     /locus_tag="NBI74_003397"
     CDS             complement(14724..14939)
                     /gene="ydgT"
                     /locus_tag="NBI74_003397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription modulator YdgT"
                     /protein_id="HCD1933679.1"
                     /translation="MTVQDYLLKFRKISSLESLEKLYDHLNYTLTDDQELINMYRAAD
                     HRRAELVSGGRLFDLGQVPKSVWHYVQ"
BASE COUNT         3710 a         3818 c         3876 g         3548 t
ORIGIN      
        1 acggtaaaaa ttggtcattc cgcgtggtat tcacgaaaag tttatgtttg agtggtgcgc
       61 aaacgtcaga ttgaaaaagg atgaactatg aaatcgggcc gctttattgg cgttatgtca
      121 ggcaccagcc ttgatggtgt tgatgttgtg ttagcgacaa ttgatgaaca ccgggtcgca
      181 cagctggcaa gtttgagctg gccgatcccg gtgtctctga aacaggctgt actggatatt
      241 tgccagggcc agcagcttac actttcgcag tttggacagc ttgatactca actcgggcga
      301 ctttttgctg atgcggtcaa tgccttgctt aaggaacaaa acctgcaggc gagagatatc
      361 gttgcgatcg gctgtcacgg tcaaaccgtc tggcacgaac cgacgggcgt ggcaccacac
      421 actttacaga ttggcgataa caatcaaatt gtggcgcgca ccggaattac ggttgtcggt
      481 gattttcgcc gtcgcgatat tgccctggga ggacaaggtg caccgctggt acctgcgttc
      541 catcatgcgc tgctggctca cccaaccgag cgacgaatgg tgctcaatat tggcggcatc
      601 gccaatctgt cactgctcat tcctggccag ccggttgggg gctacgatac cggtcctggt
      661 aacatgctga tggatgcctg gatctggcgt caggccggta aaccttacga taaagatgcc
      721 gagtgggcac gggcgggtaa agttattctc ccactgctgc aaaatatgct cagcgaccca
      781 tatttctcgc aacccgcacc gaaaagcacc ggacgcgaat actttaacta cggctggctg
      841 gagcgccatt tgcgccattt tccgggggtt gatccccgtg atgtgcaggc cacagtggca
      901 gaactcaccg ccgtgaccat ttctgaacaa gttttgttga gcggtggctg cgaacgattg
      961 atggtatgtg gtggaggtag tcgtaatccg ctactcatgg cgcgtctggc ggcattactg
     1021 ccaggcacag aagtcaccac caccgatgcc gttggcatta gtggcgatga catggaagca
     1081 ttggcgttcg cctggcttgc ctggcgaacg ctggcgggat taccaggaaa tctgccttcc
     1141 gtcactggcg caagccagga gacggtactg ggggctattt tccccgctaa cccgtgatat
     1201 cagagttaac tgaatttttc ttaccgcgtc tgccgttaca cttggaacgt tagggagggc
     1261 gtattgccct ccagaccagg aaagtcttcg ggatatgtct atgaaaaaac tgttactgat
     1321 ctgtttgccg gtgcttctca ccggctgtag cgcgttcaat caactggttg agcgcatgca
     1381 aaccgatacg cttgaatacc agtgtgatga aaaaccgttg acggtcaaac tgaataatcc
     1441 gcgccaggaa gtcagttttg tttacgataa tcaactactg catctcaaac agggcatttc
     1501 agcgtctggt gcacgttaca ccgacggaat ctatgttttc tggtcgaaag gcgaggaagc
     1561 gactgtctat aaacgcgacc gcatcgtctt gaataactgt cagttacaaa atccgcagcg
     1621 ttgagatttt tccaggggcg gcgcacaata gcgtcaccca ctgacaatcc gtaaagaaaa
     1681 ccatgtctga taacgacgaa ttgcagcaaa tcgcgcatct gcgccgtgaa tacaccaaag
     1741 gcgggttacg ccgccgcgat ctacccgccg atccattaac cctttttgaa cgttggctct
     1801 ctcaggcttg tgaagccaaa ctggctgacc ctaccgcgat ggtggtcgct accgtggatg
     1861 aacatggtca gccttatcag cgcatcgttt tactcaaaca ttacgacgaa aaaggcatgg
     1921 tgttttacac caacctcggc agccgtaaag cacatcaaat cgaaaataat ccgcgcgtta
     1981 gcctgctgtt cccgtggcat acccttgaac gccaggtgat ggtgatcggt aaagcggaac
     2041 gactttcgac tctcgaagtg atgaaatatt ttcatagccg cccgcgtgat agccagattg
     2101 gtgcatgggt ttcgaagcag tccagtcgca tttctgcccg cggtatcctt gaaagtaaat
     2161 tcctggagct gaagcagaag tttcaacagg gcgaagtgcc attgccaagt ttttggggtg
     2221 gttttcgcgt cagccttgaa cagattgagt tctggcaggg cggtgagcat cgcctgcatg
     2281 accgcttttt gtaccagcgt gaaaatgatg cgtggaagat tgatcgtctt gcaccctgaa
     2341 aagatgcaaa aatcttgctt taatcgctgg tactcctgat tctggcactt tattctatgt
     2401 ctctttcgca tctggcgaaa agtcgtgtac cggcaaaggt gcagtcgtta tatacatgga
     2461 gattttgatg gcaagcagta acttgattaa acaattgcaa gagcgggggc tggtagccca
     2521 ggtgacggac gaggaagcgt tagcagagcg actggcgcaa ggcccgatcg cgctctattg
     2581 cggcttcgat cctaccgctg acagcttgca tttggggcat cttgttccat tgttatgcct
     2641 gaaacgcttc cagcaggcgg gccacaagcc ggttgcgctg gtaggcggcg cgacgggtct
     2701 gattggcgac ccgagcttca aagctgccga gcgtaagctg aacactgaag aaactgttca
     2761 ggagtgggtg gacaaaatcc gcaagcaggt tgccccgttc ctcgatttcg actgtggaga
     2821 aaactctgct atcgcggcga acaactatga ctggttcggc aatatgaatg tgctgacctt
     2881 cctgcgcgat attggcaaac acttctccgt taaccagatg atcaacaaag aagcggttaa
     2941 gcagcgtctc aaccgtgaag atcaggggat ttcgttcact gagttttcct acaacctgtt
     3001 gcagggttat gacttcgcct gtctgaacaa acagtacggt gtggtgctgc aaatcggtgg
     3061 ttctgaccag tggggtaaca tcacttctgg tatcgacctg acccgtcgtc tgcatcagaa
     3121 tcaggtgttt ggcttgaccg ttccgctgat caccaaagca gacggcacca aatttggtaa
     3181 aactgaaggc ggcgcagtct ggttggatcc gaagaaaacc agcccgtaca aattctacca
     3241 gttctggatc aacactgcgg atgccgacgt ttaccgcttc ctgaagttct tcaccttcat
     3301 gagcattgaa gagatcaacg ccctggaaga agaagataaa aacagcggta aagcaccgcg
     3361 cgcccagtat gtgcttgcag aacaggtgac acgtctggtg catggtgaag aaggtttgca
     3421 ggcggcaaaa cgtattaccg aatgcctgtt cagcggttcc ttgagtgcgc tgagtgaagc
     3481 ggacttcgaa cagctggcgc aggacggcgt accgatggtt gagatggaaa agggcgcaga
     3541 cctgatgcag gcgctggtcg attctgaact gcaaccttcc cgtggtcagg cgcgtaaaac
     3601 tatcgcctcc aatgccatca ccattaacgg tgaaaaacag tccgatcctg aatacttctt
     3661 taaagaagaa gatcgtctgt ttggtcgttt taccttactg cgtcgcggca aaaagaatta
     3721 ctgtctgatt tgctggaaat aatgcattaa gtggaaaggg ggagtgagaa atcactcccc
     3781 ctggttttta tacagggaac atgatgaaaa atattctcgc tatccagtct cacgttgttt
     3841 atggtcatgc gggtaacagt gcggcagagt ttccgatgcg ccgcctgggc gcgaacgtct
     3901 ggccgctgaa caccgttcaa ttttctaatc acacccaata cggcaaatgg accggttgcg
     3961 tgatgccgcc cagccattta accgaaattg tgcaaggcat tgccgccatt gataaattac
     4021 acacctgtga tgccgtatta agtggctatc tgggatcggc ggagcagggt gaacatatcc
     4081 tcggtatcgt tcgtcaggtg aaagcagcga atccacaggc gaaatatttt tgcgatccgg
     4141 taatgggtca tccggaaaaa ggctgtatcg ttgcaccggg tgtcgcagag tttcatgtgc
     4201 ggcacggttt gcctgccagc gatatcattg cgccgaatct ggttgagctg gaaatactct
     4261 gtgagcatcc ggtaaataac gtcgaagaag cggttctggc agcgcgcgaa ctcattgcgc
     4321 aagggccaca aattgtgttg gttaaacacc tggcgcgagc tggctacagc cgtgaccgtt
     4381 ttgaaatgct gctagtcacc gccgatgaag cctggcatat cagccgtccg ctggtggatt
     4441 ttggtatgcg ccagccggta ggtgttggtg atgtgacgag cggtttactg ctggtgaaac
     4501 tgcttcaggg ggcaacgctg caggaggccc tggaacatgt gaccgctgca gtatacgaaa
     4561 tcatggtgac cacaaaagca atgcaggaat atgagctgca agtggttgct gctcaggatc
     4621 gtattgccaa tccagaacat tatttcagcg caacaaagct ctgaaatatc tcatttcggc
     4681 ccacaacgac ctgtgggccg atggctatta ctttaagcct tccgctgaca gcgcgtcttg
     4741 tacttccgga cgttcagcca tacgttgcat aaatgccgca atgtgctcta acccttccag
     4801 attcagtttc accgcgtatg cccagcgcag aaccgtaaac agataggcat cagcaattgt
     4861 gaatcgttgc ccgcagatcc agtgctcatc cttcagggcc tcgttcacat attgcagctt
     4921 cttctccagc tgcgcgcgaa ctgtcggttt gtactcttcc ggtgtatcag ggcgaaacag
     4981 aggtgtgaaa cctttatgca gctcggtggc gatgtaattc agccattcga tggttttata
     5041 gcgggaaata ctgtttaccg gtgccagcaa ctggcggtcg gggacgctgt cggcaagata
     5101 ctgcataatc gctacgcctt ccgtcagcaa agtaccatcg tccagcagca atgcaggtac
     5161 ctgtccctta gggttaacgg caaagtaatc gtcaccgttt tcgagacgtt ttttcattaa
     5221 atccacactg acgagcgtaa aatcctttcc gctctcacgc agggtgatat gggaagcgag
     5281 agagcaggcg cccggtttgt agaacaattt catcggtaac tccttttgtc tgtggtttaa
     5341 agtatggtag tgcgttgctg aacaaaaaaa aagccgctaa cgaaaagtta gcggcttgta
     5401 aatgagtttc cctgaatttt acgctacggc tgctttcgcc gctttgtctg cagcgtcatc
     5461 ctgcgtcatg cggtgcagtt tcggcgcggt aaacagcatc agtactgcaa taacggcagt
     5521 agcgacacca atctgcaaga atacgcgacc atagacttcc agtgacatca gcggatcggt
     5581 aacgttatcc ggcacagcca tcatacctgc aacataacca ccaatcaggt ttgcaccggc
     5641 agtggtcagg aaccagctac ccataatgaa gcccatcaga cgctgcggaa cgagttgtgc
     5701 aaccattgcc agacccaggc cagagatcat cagttccccg atactctgca ggccatagct
     5761 cgcgaccagc cagcttacag acacgatacc agcgtcagac gcgaatttcg cacccagcgg
     5821 cagaatcagg aacgcaccag aacacatcac catgccgata gcaaacttgg ttggcatcgg
     5881 cagggtatcg cccatcttgt tatagatagc ggccagaatc gggctaccaa tgatgatcca
     5941 gaacgggttc agtgcctgat actgctcagg ttctacggcc aggcccagaa tggtgtgttc
     6001 aacgttacga atcgcaaaga agttcagtga cgttggcatc tggctataaa gcacgaagaa
     6061 gataatggct tcgagcatca ggatgaaggc aacgatcatt ttacgacgcg cagcaccttt
     6121 catcgcgaag gcttctttac cgaagataac cacaataccg aaggcaacaa cgcccagcgc
     6181 catacgcgca acttcctgat tgtgcagcag ccaggtggcg atagcgatca gtgccacaac
     6241 accaataatg gtcagcagca ggttacggta gttgataggc tcgaagtctg gttttgaacc
     6301 gtactgttta acccaacgtt ggcagaaggc gaagttaacg atagtgatca gcaggcctac
     6361 aacgctcaac gcaaacgcaa cactccagcc gtatttcgcg gccagccacg gcgtagcaat
     6421 catagagaag aaagagccga tgttgacgga catgtagtac atggtgaatg caccgtccag
     6481 acgcgggtcg tttttctcat agcatgtaga aagcagagaa gacgggttag ctttaaacag
     6541 gccgttaccg accgcaatag ccgccatacc catataaacg ataccggcgt cgtgaccaga
     6601 ccaggcaacc agagcataac caatcgccag cacaatagcg ccgagcataa ttacgcgttt
     6661 agtacccagt accttgtcac ctaaccagcc gccgatagcg accagaccat aaaccagggc
     6721 actaaaggaa gagaaaaggg tgattgaatc cgcttcagac atacccagtt gtttaaccag
     6781 gtaaacagcc ataattcctt gtaggccgta ataaccaaaa cgttcccata actcaatcga
     6841 gaagatgaga tagaacgcct tcggttgttt aaaagcgttc aaactgacgc tttcagttgg
     6901 tttttggttt gcagtggaca cttttacctc tttttttaca tcccatatta acgggggtgc
     6961 tttctcgcgt tatgtctaaa aaacatacgc gcaattgtta tagtgggagg ggaaacggca
     7021 ggtaatgttc actatcttgc actttcagac aagtcctttg taataatctg ttacatataa
     7081 ctgaggcggg gaattcaacc attccgggaa atgttatcca gcgttaaatt tttcaaaaaa
     7141 cgtaacggca gattttttca ctaccctgag agggtgatga tggcttaacg gcgatatgtt
     7201 ctactattta gcgtttttcc tagtgagata gtccagtttc tgaaaaatag ccagtgtaat
     7261 gttttgtagg tcaatatttg ttgtgtgtgt aaatttaaat gattttaacc tgattcttgc
     7321 aatgttttta cattgccaca gcatcgtgat cttgatcacg aatgaataga aaatttcgct
     7381 attaaaaaaa caattaacct gcatttttgg ttatctgcaa acggattacc tgatgataac
     7441 gctaagcaat ggcatcattg acgttaaaaa gatgttttaa aagaggataa agaaaggtta
     7501 tgaatggggt aatcggtgtt acccctgatc tttagatgtc aactttctct ttgtattcac
     7561 aaagatcttc aataatacaa gagccacagc gaggcttgcg ggcaatgcag gtataacgcc
     7621 cgtgcaggat caaccaatgg tggcagtcga ctttaaactc tgccggaacc actttcagta
     7681 gcttttcttc tacctgttcg acgtttttcc ccggcgcaaa ttgagtacga ttgcaaacgc
     7741 ggaaaatgtg cgtgtcgaca gcaatagtcg gccagccgta tgcagtgttt aatacgacgt
     7801 tggctgtttt acgacctaca ccaggcaggg cttcaagcgc agcacgatct tccggaacct
     7861 cgccattatg ttgttccagt aatatacggc aggttttgat gatattttct gctttgctgt
     7921 tataaaggcc aatcgttttg atataggttt tcaccccttc aacgcccagt tcaagcatcg
     7981 ctgcaggcgt attagccacc gggtagagtt tcgccgtcgc cttattaaca ctgacatcgg
     8041 tcgcctgagc ggaaagcagt acggcaatca gcaattcaaa aggcgaactg aaattaagct
     8101 cggtggtggg atgaggattg ttctcacgca ggcgagtgag gatctccagg cgttttgctt
     8161 tgttcatcag acattccctg tttcaccgtt tggcaatgca cgttcggcag ctgcttcagt
     8221 acggcgcttt ttcatttttt catcaatcag gtattttcct gccagcatca gtcccaggcc
     8281 aataaatgca cctggtggca gcatcgccag caggaaaggg gagtcggtgt ggaaaatctc
     8341 cacgcgtaat acttttgccc agctacctaa cagcgcatct gcaccgtcaa acaacgtgcc
     8401 attgccgata atttcgcgta gtgaacccag cacgaacatg gcgcaggttg cgcccatacc
     8461 aattgaaaag ccgtccagtg ccgaaagcgc cggacctttt ttggcggcga aggcttcagc
     8521 gcggcccaca acgatacagt tagtgacaat cagcggaata aaaatcccta atgattgata
     8581 caggccaaag gcgtaggcgt tgatcagcat ctgtacagcg ctgaccaccg aggcgatgat
     8641 catcacgtaa atgggaatgc ggatctcggc gggcgtccag tgacgcagcg tcgaaatggt
     8701 caggttggtt agcgtcagta ccagcgtagt cgcaagtcct aaacccagag cgttagtggc
     8761 agtggacgtg accgccaaca gaggacaaag gccgagcagc tggaccagcg cagagttgtt
     8821 tttccacaac ccctgaacaa taacgtcttt aatttcgctc acgttttatt ctccacaggc
     8881 aggaagttga gaaagttgtg ccggtaacgt ctgagcgtac aatccggcgc gttttaccgc
     8941 attaaccacc gcgcggggag taatcgtcgc gccagtgaac tggtcgaaat caccaccatc
     9001 tttcttcacc gcccagttcg catcatccgc accactgatt tttttacccg caaaatgggt
     9061 gatccagtca gaaaggcgca gttcgatttt atcgccaagc cctggcgttt cgtggtgctc
     9121 agtcacgcgc gtgccaagta ccgtgccgtt aaaatcggct cccaccagca gctgaatcgc
     9181 accggaatag ccatctggcg cggttgcttc cagaacggca gctaccggtt tgtcatcctg
     9241 tttggcgatg taaacccgat gctcaccttt acctaactct ggcgcagtta ccagatagca
     9301 actctgtgcc agcgcattgt tatagcgttc agctggcagc acctgatcaa ataacgcctt
     9361 ttgttgcaga ctggcctgct cagcaattgt cgttttggtc atctggttga tggccgcagt
     9421 taaccctgtt gaacccgctg caaagagcgc caacgtaatg ccgtgttttc ggatagtttt
     9481 cagcatggtt taccctttgc gatggccgta gacgcgcgga cgcgtgtagt aatcgatcag
     9541 aggaaccgtg atgttcgcca gcaggacggc aaaagccacg ccgtcaggat agccgccgaa
     9601 actgcggatc aaccagacta ataagcccgc cagcgcgccg aaaataagac gaccacgatt
     9661 ggtcgtagaa gcggtaaccg ggtcagtcaa aataaagaat gcgccgagca tggtcgcacc
     9721 agacagcaga tgaatttgcg gtgctgccag agtatctggt gagaacaacc agcccaacgt
     9781 tgcgcataac gccagcgtta ctaagaagct gaggggaata tgccagcgaa tcgctttctg
     9841 ccatagcaac cacacgccgc cagtcagcca ggcgagattt acccattgcc aaccagcgcc
     9901 cgccagaata ccgctgtaga tcggatattg cataatctct tcaaccgaat gaccggcacg
     9961 gacagaggtt ttaaatgtat ccagcggtgt cgcctgacta atgccatcaa tacctaagcg
    10021 tagcgtgttc atatcaccac cactggtggt atgtccgctg aaaataacct gtatggcgtc
    10081 gataaaacca gggatgttga ccgcaatttc atgtggcggt aaccagctgg tcatctgcac
    10141 cgggaaggag atcagtaaga ccacataacc aatcattgcc ggattaaacg ggttttgccc
    10201 cagaccgcca tacaactgtt tagcgatgat caccgcaaac accgtaccca gcacgaccat
    10261 ccaccatggc gcgagggggg gaatacttac cgccagcaat aagcctgtca gcaatgctga
    10321 gttatctttc aacgttgcag ctaccgactg cttgcgtagt ttgaggacga gagcttcggc
    10381 cagaagagca ctgaccgacg ccaacaggat ctgaacgaga gtaccccaac caaaaaacca
    10441 cagttgcgct gcgattcctg gcacggctgc gagcaatacc aacatcataa tgcgcgatgt
    10501 ctggcgctgg ttatgggtat aaggggagct agctattctg aataccattt agtcctcgtt
    10561 tacaaccttt tgctgggcgg cttttttggc ctgaacgcgg gcaatagccg cagcaactgc
    10621 cgctttgcgc ggatcaatct gttcttctgg ctctgcatta gcctgttgct gttccagctt
    10681 gcgtgctttg gcacgggcaa tagcagcttc gacggcggct ttgcgtggat caacctgttc
    10741 ttctggttcc gcattagcct gttgttgctg ttccagcttg cgcgctttgg cgcgtgcaat
    10801 agccgcttcg acggcagctt tgcgcggatc gacctgttgt tctggctccg cattggcctg
    10861 ttgctgttcc agcttgcgtg ctttggcgcg ggcgatagcg gcttcgacgg cggctttgcg
    10921 tggatcaacc tgttcttctg gttccgcatt agcctgttgt tgctgttcca gcttgcgtgc
    10981 tttggcacgg gcaatagcag cttcgacggc ggctttgcgt ggatcaacct gttcttctgg
    11041 ttccgcattg gcctgttgct gttccagctt gcgcgctttg gcgcgtgcaa tagccgcttc
    11101 gacggcagct ttgcgcggat cgacctgttg ttctggttcg gcattagcct gttgctgttc
    11161 cagcttgcgt gctttggcac gggcaatagc ggcttcgacg gcggctttgc gcggatcaac
    11221 ctgttgttct ggctccgcat tggcctgttg ctgctccagc ttgcgtgctt tggcgcgggc
    11281 aatagccgct tcaacggcag ttttacgtgg atcagcaacg gttgcagcgt cgttagtttg
    11341 ctgcagttct gcctgtttcg ctctggcttg cgctttacgg gcttcccgtg ctgcaataat
    11401 tgcactgtta tccgggcgtt cgcccgcttt aatcacaata ggctgtgtag cctgggcctg
    11461 tttctctttc acccgcgcca gagcggcagc aatcgcatct ttatctttgg ctgcaggttg
    11521 aacggctgcg ctcttatgtc gttcaaggcg agccgttttt tcgcgctcca gacgagcctg
    11581 gcgcgcttcg aaacgcgctt tggcttctgc ggcgcgcttt tcttcctgac gaatagccgc
    11641 aatttcagct ttttcctgac ggaaatattg caccaggggg atattgctcg ggcaaaccca
    11701 cgcgcaagcc ccacattcaa tgcagtcggc aatgttatgc gtggtggctt tatcgtgttg
    11761 ctgacctttg ctgaaccagt acagctgttg cggcaaaaga tccgcagggc aggcgtcagc
    11821 acaggcgcta caacggatgc aactttgttc ttcctgtggt tcgccaagtt cattggcaga
    11881 gggagccaaa agacagttgg taatctttac gaccgggaca tccagccacg gtaaggtaaa
    11941 gcccattagc gggccgccca taatcaccat ttgatcggca gaggggcaga atccggcatc
    12001 attcaataaa tgacgcactg gcgtacccag ccgtgcccag acgttgcccg ggcgagcgat
    12061 tgcttcgcca gtcagggtca cgacacgctc ggtaatcggc tcgccatcaa taacggcacg
    12121 tttcactgca taagcggtgc cgacgttttg cattaatacg ccgatatcgg atgaacgccc
    12181 gccatgtgga acctgcttcc cggtcagaat gtaggttaat tgtttagcac cgccagaagg
    12241 atatttggtg ggaatcaccc gcagagaaat atcgtgggag tccgccagca ccgcgcgtag
    12301 catggaaatc gcctgcggtt tgttatcttc aatgccgata agaatttcgc gcggctgcag
    12361 aatatgcgca aggatgcgga taccctctac gacctgagcc gcgcaatcct gcatcaaacg
    12421 gtcatcggcg gtaatgtacg gctcgcactc agcagcgttg ataatcaacg tttcaatctt
    12481 atctccgccg ccctgcaatt taacgcctgt cggaaatccc gcaccaccca gcccggcaac
    12541 accaaactga tgaatgcgct cgattaactc ttcgcgactg cgagagcgat aatcggccca
    12601 gccgtcgcgc gggatccagc agtcttcgcc atcggcatca ataatcacac ttaattcagc
    12661 taaagctgaa ggatgagccg tagagtgggg cgcaatagcc gtaacggtac ccgaggtggg
    12721 cgcgtgaacc ggcagcattt tgccgcgacc acgggtaagc ggctggccgc gcaatacttt
    12781 atcgccgacg ctaacgcaca actcaccttc agcgccaata tgttgtttta gtggaatgac
    12841 aaaacgctgc gccagtggta cctggcgcag gggcgtaccg ttggactggg ttttcatctc
    12901 cggtggatgg atgccgccgt tgaaatccca gattttattt tttctgaatg cagagaataa
    12961 cttaagcatg gtgttccacg ggaatgatac gcacgggaat ggtgttcaga tcccatttcc
    13021 aggagtcagg agtttctgcg accggttgca acgagatgca gtgcgtcggg cacggatcaa
    13081 cacataaatt gcagcccgta cagagatcac tcattaccgt atgcatggcg cgggtagcgc
    13141 caacgatggc gtctaccgga cacgcctgaa tacatttagt gcagccaata cagttatttt
    13201 catcaataac cgccaccatc cgcgctggcg ttagctcttg cgcttcgcca tccagcggct
    13261 gcggctcgac attaagcaac tcggcaattt ttagcatcac agcttcgcca cctggggcgc
    13321 aacggttgat tttttcaccg ttacagctga tggcttccgc gtaggggcga cagccgggat
    13381 aaccgcactg accacactgg ctctgcggta agatttcgtc aattttctca acgaccggat
    13441 cgtcttccac cgcaaaacgg cgggaggcat aacccagaat ggcgccaaac gccagggcca
    13501 gcaggctcac ggcggcaacg gcaatccaga tagcattcat tacaacttca ccaaaccact
    13561 aaagcccata aaggccagag acataagacc tgcggtaatt aacgcaatgg cattaccgcg
    13621 aaaaggtgcc gggacatcag ccacagcaag gcgttcgcgg atggcggcga agagcaccat
    13681 caccagcgag aaaccgacag cggcggaaaa accgtacagc gctgactgca agaaattgtg
    13741 cccgagattg atattcagca acgccacgcc gagcacggca cagttggtgg tgataagcgg
    13801 caaaaaaatc cccagcagac ggtaaagtac cgggctggtt ttgcgcacca ccatttcggt
    13861 gaactgcaca accacagcaa tcaccagaat aaacgccagg gtgcgcagat aaatcagatt
    13921 aagtgggatc aaaatccacg tatcgataag ccaggcgcaa atagacgcca gcgtcattac
    13981 aaacgttgtt gccagcccca tgcccatcgc ggtttccagc tttttggaaa cccccataaa
    14041 cggacagaga ccgagaaact tgaccagtac aaagttattg accagtacag ttccgacaaa
    14101 cagtaacagg tagtcagtca tttttcggcc tgaaataaaa aagccgccta ttatccgtta
    14161 atccagagca ggcgacaaca ggttaactgt aaggttatta cggattcaca aacgtcgctt
    14221 tcacccgcga cgagcgtttg aaatagggga cgatcaatgc ggttgccaac aaagtaaaca
    14281 gcaactggcg aacggctatg ccatcttcta cgggtgaaaa agcgaacgct tttactgcca
    14341 gcaatacgga aataagcagc cagataatat agtgtttagg aacacaacgc cgacgtttaa
    14401 agaaagcgat ggtcaaccac agtgtgtaat accacatggc gatagcggta ataaaagaaa
    14461 cgccccataa gatttgtgtc gtcagtgctt gtccgccgag cgtttgaaat gtttgcggag
    14521 aagataacgc agcggtatac agcaacaacg ccagcgtcgt actcaacaga gcgactaaaa
    14581 gccaggccaa cgggccaagc aaccagcctc caatacgttg tggcgttgtg gtggtcatag
    14641 gttctccaga atagcgacgc aaacagacaa atctaatttt aggggcgtta ttataaggct
    14701 tttataaccg atcgctactt tctttactgg acatagtgcc agacggactt cggtacctgg
    14761 ccgaggtcaa acaaacgccc gccagaaacc agctctgcgc gacggtgatc ggcagcacga
    14821 tacatattga tcagttcctg atcgtccgtc agggtgtaat taagatggtc gtagagtttt
    14881 tccagacttt cgagtgaact gattttgcga aattttaata agtagtcctg aacagtcata
    14941 aaaggtccat aa
//
LOCUS       DAHHTG010000107        14910 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.108, whole genome shotgun sequence.
ACCESSION   DAHHTG010000107 DAHHTG010000000
VERSION     DAHHTG010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14910)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14910)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14910
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.108"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            complement(295..1944)
                     /gene="pgi"
                     /locus_tag="NBI74_003398"
     CDS             complement(295..1944)
                     /gene="pgi"
                     /locus_tag="NBI74_003398"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HCD1933680.1"
                     /translation="MKNINPTQTAAWQALQKHFDEMKDVTIADLFAKDGDRFSKFSAT
                     FDDQMLVDYSKNRITEETLAKLQDLAKECDLAGAIKSMFSGEKINRTENRAVLHVALR
                     NRSNTPILVDGKDVMPEVNAVLEKMKTFSEAIISGEWKGYTGKAITDVVNIGIGGSDL
                     GPYMVTEALRPYKNHLNMHFVSNVDGTHIAEVLKKVNPETTLFLVASKTFTTQETMTN
                     AHSARDWFLKAAGDEKHVAKHFAALSTNAKAVGEFGIDTANMFEFWDWVGGRYSLWSA
                     IGLSIVLSIGFDNFVELLSGAHAMDKHFSTTPAEKNLPVLLALIGIWYNNFFGAETEA
                     ILPYDQYMHRFAAYFQQGNMESNGKYVDRNGKVVDYQTGPIIWGEPGTNGQHAFYQLI
                     HQGTKMVPCDFIAPAITHNPLSDHHQKLLSNFFAQTEALAFGKSREVVEQEYRDQGKD
                     PATLDYVVPFKVFEGNRPTNSILLREITPFSLGALIALYEHKIFTQGVILNIFTFDQW
                     GVELGKQLANRILPELKDDKEISSHDSSTNGLINRYKAWRG"
     gene            2469..3818
                     /gene="lysC"
                     /locus_tag="NBI74_003399"
     CDS             2469..3818
                     /gene="lysC"
                     /locus_tag="NBI74_003399"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-sensitive aspartokinase 3"
                     /protein_id="HCD1933681.1"
                     /translation="MSEIVVSKFGGTSVADFDAMNRSADIVLSDANVRLVVLSASAGI
                     TNLLVALAEGLEPGERFEKLDAIRNIQFAILERLRYPNVIREEIERLLENITVLAEAA
                     ALATSPALTDELVSHGELMSTLLFVEILRERDVQAQWFDVRKVMRTNDRFGRAEPDVA
                     ALAELAALQLLPRLNEGLVITQGFIGSENKGRTTTLGRGGSDYTAALLAEALHASRVD
                     IWTDVPGIYTTDPRVVSAAKRIDEIAFAEAAEMATFGAKVLHPATLLPAVRSDIPVFV
                     GSSKDPRAGGTLVCNKTENPPLFRALALRRNQTLLTLHSLNMLHSRGFLAEVFGILAR
                     HNISVDLITTSEVSVALTLDTTGSTSTGDTLLTQSLLMELSALCRVEVEEGLALVALI
                     GNDLSKACGVGKEVFGVLEPFNIRMICYGASSHNLCFLVPGEDAEQVVQKLHFNLFE"
     gene            4070..4342
                     /locus_tag="NBI74_003400"
     CDS             4070..4342
                     /locus_tag="NBI74_003400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3811 domain-containing protein"
                     /protein_id="HCD1933682.1"
                     /translation="MALPRITQKEMTEREQRELKTLLDRARIAHGRVLTNSETNSIKK
                     EYIDKLMVEREAEAKKARQLKKKQAYKPDPEASFSWSANTSTRGRR"
     gene            complement(4343..5215)
                     /gene="rluF"
                     /locus_tag="NBI74_003401"
     CDS             complement(4343..5215)
                     /gene="rluF"
                     /locus_tag="NBI74_003401"
                     /EC_number="5.4.99.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2604) synthase RluF"
                     /protein_id="HCD1933683.1"
                     /translation="MLPDSSVRLNKYISESGICSRREADRYIEQGNVFLNGKRATIGD
                     QVKSGDVVKVNGQLIEPREAEDLVLIALNKPVGIVSTTEDGERDNIVDFVNHSKRVFP
                     IGRLDKDSQGLIFLTNHGDLVNKILRAGNDHEKEYLVTVDKPITDEFIRGMGAGVPIL
                     GTVTKKCKVKKEAPFVFRITLVQGLNRQIRRMCEHFGYEVKKLERTRIMNVSLSGIPL
                     GEWRDLTDDELIDLFKLIENSSSEAKPKAKAKPKTVGIKRPVVKMEKTAEKGGRPASN
                     GKRFTSPGRKKKGR"
     gene            5563..6510
                     /locus_tag="NBI74_003402"
     CDS             5563..6510
                     /locus_tag="NBI74_003402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HCD1933684.1"
                     /translation="MENSDDIRLIVKIAQLYYEQDMTQAQIARELGIYRTTISRLLKR
                     GRDQGIVTIAINYDYNENLWLEQQVKQKFGLKDVVVVSGNDEDEDTQLAMMGLHGAQL
                     LDRLLEPGDIVGFSWGRAVSALVENLPQAGQSRQLICVPIIGGPSGKLESRYHVNTLT
                     YSAAAKLKGESHLADFPALLDNPLIRNGIMQSQHFKTISAYWDNLDVALVGIGSPAIR
                     DGANWHAFYGGEESDDLNARQVAGDICSRFFDIHGAMVETNMSEKTLSIEMNKLKQAR
                     YSIGIAMSEEKYSGIVGALRGKYINCLVTNSSTAELLLK"
     gene            6580..7386
                     /locus_tag="NBI74_003403"
     CDS             6580..7386
                     /locus_tag="NBI74_003403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCD1933685.1"
                     /translation="MQTWLNLQDKIIIVTGGASGIGLAIVDELLAQGANVQMVDIHGG
                     DGQYESHKGYQFWPTDISSAKEVNHTVAEIIQRFGRIDGLVNNAGVNFPRLLVDEKAP
                     AGQYELNEAAFEKMVNINQKGVFLMSQAVARQMVKQHDGVIVNVSSESGLEGSEGQSC
                     YAATKAALNSFTRSWSKELGKHGIRVVGIAPGILEKTGLRTPEYEEALAWTRNITVEQ
                     LREGYTKNAIPIGRAGRLAEVADFVCYLLSERASYITGVTTNIAGGKTRG"
     gene            7396..7803
                     /locus_tag="NBI74_003404"
     CDS             7396..7803
                     /locus_tag="NBI74_003404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sorbose transporter subunit IIA"
                     /protein_id="HCD1933686.1"
                     /translation="MVNAIFCAHGRLACAMLESVQMVYGDARVEAVEFVPGENAGDIV
                     AKLEKLVSIHNHDEWLIAVDLQCGSPWNAAAMLAMRNPRLRVISGLSLPLALELVDNQ
                     DSMNVDELCEHLTQIAKQTCVVWRQVATAEEDF"
     gene            7803..8297
                     /locus_tag="NBI74_003405"
     CDS             7803..8297
                     /locus_tag="NBI74_003405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HCD1933687.1"
                     /translation="MNITLARIDDRLIHGQVTTVWSKVANAQRIIICNDEVYNDEVRR
                     TLLRQAAPPGMKVNVVNIEKAVAVYHNPQYQDETVFYLFTRPQDALAMVRQGVKIGIL
                     NIGGMAWRPGKKQLTKAVSLDDDDINAFHELNNLGVILDLRVVASDPSINIIDKINEQ
                     LIAN"
     gene            8363..9160
                     /locus_tag="NBI74_003406"
     CDS             8363..9160
                     /locus_tag="NBI74_003406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HCD1933688.1"
                     /translation="MEISTLQIIAIFLFSCIAGMGSVLDEFQTHRPLIACTVIGLILG
                     DLKTGIMLGGTLELIALGWMNVGAAQSPDSALASIISAILVIVGQQSIATGIAIALPV
                     AAAGQVLTVFARTITVVFQHAADKAAEEARFRTLDILHVSALGVQALRVAIPALIVSL
                     FVSADMVSNMLSAIPEFVTRGLQIAGGFIVVVGYAMVLRMMGVKYLMPFFFLGFLAGG
                     YLDLSLLAFGGVGVIMALLYIQLNPQWRKAEPHPQTTTITALDQLDD"
     gene            9171..9995
                     /locus_tag="NBI74_003407"
     CDS             9171..9995
                     /locus_tag="NBI74_003407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HCD1933689.1"
                     /translation="MEQRKITRSDLVSMFLRSNLQQASFNFERIHGLGFCYDMIPAIK
                     RLYPLKEDQVAALRRHLVFFNTTPAVCGPVIGVTAAMEEARANGAEIDDGTINGIKVG
                     LMGPLAGVGDPLVWGTLRPITAALGASLALSGNILGPLLFFFIFNAVRLAMKWYGLQL
                     GFRKGVNIVSDMGGNLLQKLTEGASILGLFVMGVLVTKWTSINVPLVVSQTPAADGAT
                     VTITVQNILDQLCPGLLALGLTLLMVRLLNKKINPVWLIFALFGLGIIGNALGFLS"
     gene            10047..11276
                     /gene="sorE"
                     /locus_tag="NBI74_003408"
     CDS             10047..11276
                     /gene="sorE"
                     /locus_tag="NBI74_003408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-sorbose 1-phosphate reductase"
                     /protein_id="HCD1933690.1"
                     /translation="MKTTALRLYGKRDLRLETFDLPEMQEDEILATVVTDSLCLSSWK
                     EANLGENHKKVPDDVATNPIIIGHEFCGDILAVGKKWQHKFQPGQRYVIQANLQLPDR
                     PDCPGYSFPWVGGEATHVVIPNEVMEQDCLLAYDGETYFEGSLVEPLSCVIGAFNANY
                     HLQEGSYNHTMGIRPQGRTLILGGTGPMGLLAIDYALHGPVNPSLLVITDTDNDKLSY
                     ARKHYPSEPQTLIHYLNAADAAFDTLMALSGGHGFDDIFVFVPNEGLVTLASSLLATD
                     GCLNFFAGPQDKHFSAPINFYDVHYAFTHYVGTSGGNTDDMRAAVKLIEEKKVQAAKV
                     VTHILGLNAAGETTLELPAVGGGKKLVYTGKYLPLTSLTQIQDQALAAILARHQGIWS
                     GEAEQYLLAHAEAISHD"
     gene            11658..12347
                     /gene="pepE"
                     /locus_tag="NBI74_003409"
     CDS             11658..12347
                     /gene="pepE"
                     /locus_tag="NBI74_003409"
                     /EC_number="3.4.13.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepE"
                     /protein_id="HCD1933691.1"
                     /translation="MELLLLSNSTLPGKAWLEHALPLIAEQLQGRRSAVFIPFAGVTQ
                     TWDDYTAKTAAVLAPLGVSVTGIHSVVDPVAAIENAEIVIVGGGNTFQLLKQCRERGL
                     LAPITDVVKRGALYIGWSAGANLACPTIRTTNDMPIIDPQGFDALNLFPLQINPHFTN
                     ALPEGHKGETREQRIRELLVVAPELTIIGLPEGNWITVSKGHATLGGPNTTYVFKAGE
                     EAVPLEAGHRF"
     gene            complement(12437..14068)
                     /locus_tag="NBI74_003410"
     CDS             complement(12437..14068)
                     /locus_tag="NBI74_003410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HCD1933692.1"
                     /translation="MLTLLHLLSAVALLVWGTHIVRTGVMRVFGARLRTVLSRSVEKK
                     PLAFCAGIGVTALVQSSNATTMLVTSFVAQDLVALAPALVIVLGADVGTALMARILTF
                     DLSWLSPLLIFIGVIFFLGRKQSRAGQLGRVGIGLGLILLALELIVQAVTPITQANGV
                     QVIFASLTGDILLDALIGAMFAIISYSSLAAVLLTATLTAAGIISFPVALCLVIGANL
                     GSGLLAMLNNSAANAAARRVALGSLLFKLVGSLIILPFVHLLAETMGKLPLPKAELVI
                     YFHVFYNLVRCLVMLPFVDPMARFCKTIIRDEPELDTQLRPKHLDVSALDTPTLALAN
                     AARETLRIGDAMEQMMEGLNKVMHGEPRQEKELRKLADDINVLYTAIKLYLARMPKEE
                     LAEEESRRWAEIIEMSLNLEQASDIVERMGSEIADKSLAARRAFSLDGLKELDALYEQ
                     LLSNLKLAMSVFFSGDVTSARRLRRSKHRFRILNRRYSHAHVDRLHQQNVQSIETSSL
                     HLGLLGDMQRLNSLFCSVAYSVLEQPDEDEGRDEY"
     gene            complement(14239..14688)
                     /locus_tag="NBI74_003411"
     CDS             complement(14239..14688)
                     /locus_tag="NBI74_003411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001189559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1933693.1"
                     /translation="MQRQYKKYILLFWGVMDVIAIATYLFYSLGNGRIPFYSDIVHSL
                     SLLKDIGVEGGFYAYAVATVTLQIVLLFSLFFSAQCFLRQKLFSFSFLAIQEFMRFAT
                     VSCSISVIPLLLNYFYSQDLVLNISLFIFSEVTKVTTIIWCKRQGKV"
BASE COUNT         3698 a         3720 c         3831 g         3661 t
ORIGIN      
        1 ttactctgcg tattttccga aataaaccac gattaaaaat ttattttagg attaacctca
       61 cggtatgatt ttcgttaaat gacagacaag aggatttatc tgataaaaca atgccgactt
      121 atagtcggca ttgtttttaa ggctatttaa tcaatcgtac tatgtattaa ttgccgaata
      181 cgatgtacat atcggcatcg acctgtaggc ctgataagac gcgaccgcgt cgcatcaggc
      241 atcggttgcc ggatgcggcg tgaacgcctt atccggccta catatcgaca atgattaacc
      301 gcgccacgct ttatagcggt taatcagacc attggtcgag ctatcgtggc tgctgatttc
      361 tttgtcatct ttcagctctg gcagaatacg gttcgccagt tgtttaccca gctccacgcc
      421 ccattggtcg aaggtgaaga tgttcaggat cacgccctga gtaaagattt tgtgctcata
      481 cagcgcaatc aacgccccca ggctgaacgg agtgatttca cgcagcagga tggagttggt
      541 cgggcggtta ccttcgaaca ctttgaacgg caccacgtag tcaagcgttg ccggatcttt
      601 accctgatcg cgatattcct gctcaaccac ttcgcgggat ttaccaaacg ccagcgcttc
      661 ggtctgagca aagaagttag acagcagttt ctggtgatga tcagagagcg ggttatgggt
      721 gatagccgga gcgatgaaat cgcacggtac cattttggtt ccctggtgga tcagctgata
      781 gaacgcgtgc tgaccgttcg tgccaggttc accccagata atcgggccag tctggtaatc
      841 cacaacctta ccgttacggt caacatactt accgttggat tccatattgc cctgctggaa
      901 gtacgccgcg aaacggtgca tatactggtc atacggcaga atcgcttcag tttccgcgcc
      961 aaagaaattg ttgtaccaga tgccaatcag cgccagcagt acaggcaggt ttttctcggc
     1021 aggcgtggtg gagaaatgct tgtccatcgc gtgtgcgcca gaaagcagtt caacgaagtt
     1081 atcaaagccg atggagagaa caatcgacag gccaatcgct gaccacaaag aataacgacc
     1141 gccgacccag tcccagaatt cgaacatgtt ggcagtatca ataccaaact cgccaacggc
     1201 tttggcgttg gtggaaagcg ccgcaaagtg tttcgcaacg tgcttctcat cacctgccgc
     1261 tttcaggaac cagtcacgcg cgctatgggc gttggtcatg gtttcctgag tggtgaaggt
     1321 tttagatgct accaggaaca gcgtggtttc cgggtttact tttttcagca cttccgcgat
     1381 gtgagtccca tcgacgttag aaacaaagtg catgttcagg tggtttttgt atggacgcag
     1441 tgcttcagtc accatgtacg ggccgaggtc agaaccgccg atcccgatgt tcactacgtc
     1501 agtgattgcc ttgccggtat agcctttcca ctcaccggaa ataatcgctt ccgagaaggt
     1561 tttcatcttc tccagcaccg cgttgacttc cggcataaca tctttgccat caaccagaat
     1621 cggggtattg ctacggttac gcagcgctac gtgcagtacg gcgcggtttt cagtgcggtt
     1681 gatcttctcg ccagagaaca tcgacttaat cgcgcccgcc agatcgcact ctttcgccag
     1741 atcttgtaat ttcgccagcg tctcttcagt gatgcggttt ttggagtaat ccaccagcat
     1801 ctgatcgtcg aaggttgcgg agaacttaga aaaacgatcg ccgtctttag caaaaagatc
     1861 ggcgatcgta acgtctttca tttcatcgaa gtgtttctgt agtgcctgcc aggcagcggt
     1921 ctgcgttgga ttgatgtttt tcattagcaa tactcttctg attttgagaa ttgtgacttt
     1981 ggaagattgt agcgccagtc acagaaaaat gtgatggttt tagtgccgtt agcgtaatgt
     2041 tgagtgtaaa cccttagcgc agcgaagcat ttattagctg aactactgac cgccaggagt
     2101 ggatgaaaaa tccgcatgac cccatcgttg acaaccgccc cgctcaccct ttatttataa
     2161 atgtactacc tgcgctagcg caggccagaa gaggcgcgtt gcccaagtaa cggtgttgga
     2221 ggagccagtc ctgtgataac acctgagggg gtgcatcgcc gaggtgattg aacggctggc
     2281 tacgttcatc atcggctaca ggggctgaat cccctgggtt gtcaccagaa gcgttcgcag
     2341 tcgggcgttt cgcaagtggt ggagcacttc tgggtgaaaa tagtagcgaa gtatcgctct
     2401 gcgcccaccc gtcttccgct cttcccttgt gccaaggctg aaaatggatc ccctgacacg
     2461 aggtagttat gtctgaaatt gttgtctcca aatttggcgg taccagcgta gctgattttg
     2521 acgccatgaa ccgcagcgct gatattgtgc tttctgatgc caacgtacgt ttagttgtcc
     2581 tctcggcttc tgctggtatc actaatctac tggtcgcttt agctgaagga ctggaacctg
     2641 gcgagcgatt cgaaaaactc gacgcaatcc gcaatatcca gtttgccatt ctggaacgtc
     2701 tgcgttaccc gaacgttatc cgtgaagaga ttgaacgtct gctggagaac attactgttc
     2761 tggcagaagc ggcggcgctg gcaacgtctc cggccctgac agatgaactg gtcagccacg
     2821 gcgagctgat gtcgaccctg ctgtttgtcg agatcctgcg cgaacgcgat gttcaggcac
     2881 agtggtttga tgtacgtaaa gtgatgcgta ccaacgatcg atttggtcgt gcagagccag
     2941 atgtagccgc gctggcggaa ctggccgcgc tgcagctgct tccacgcctc aatgaaggct
     3001 tagtgatcac ccagggattt atcggcagcg aaaataaagg tcgtacaacg acgcttggcc
     3061 gtggaggcag cgattatacg gcagccttgc tggcggaggc tttacacgca tctcgtgttg
     3121 atatctggac cgacgtcccg ggcatctaca ccaccgatcc gcgcgtggtt tccgcagcaa
     3181 aacgcattga tgaaatcgcg tttgccgaag cggcagagat ggcaactttt ggtgcaaaag
     3241 tactgcatcc ggcaacgttg ctacccgcag tacgcagcga tatcccggtc tttgtcggct
     3301 ccagcaaaga cccacgcgca ggtggtacgc tggtgtgcaa taaaactgaa aatccgccgc
     3361 tgttccgcgc gctggcgctt cgtcgcaatc agactctgct cactttgcac agcctgaata
     3421 tgctgcattc tcgcggtttc ctcgcggaag ttttcggcat cctcgcgcgg cataatattt
     3481 cggtagactt aatcaccaca tcagaagtga gcgtggcatt aacccttgat accaccggtt
     3541 caacctccac tggcgatacg ttgctgacgc aatccctgct gatggagctt tccgcactgt
     3601 gccgggtgga agtggaagag ggtctggcgc tggtcgcgtt gattggcaat gacctgtcaa
     3661 aagcctgcgg cgttggcaaa gaggtattcg gcgtactgga accgttcaac attcgcatga
     3721 tttgttacgg cgcatccagc cataacctgt gcttcctggt gcccggcgaa gatgccgagc
     3781 aggtggtgca aaagctgcat tttaatttgt ttgagtaaac acagtatggc ctggaagcta
     3841 tatttcgggc cgtattgatt ttcttgtcat tatgcccata aataaacgag cctgtactct
     3901 gttaaccagc gtctttatcg gagaataatt gcctttaatt ttttatctgc atctctaact
     3961 aattatcgaa agagataaat agttaagaga aggcaaaatg aatattatca gttctgctcg
     4021 caaacgaatt cccgtacact gaactctctg gctcactgag ggcaccatca tggcactccc
     4081 ccgtattacc caaaaagaga tgactgaacg cgagcagcgt gaacttaaaa cactgctgga
     4141 tcgggcgcgg attgcgcatg gtcgcgtact gaccaactcc gaaaccaaca gcattaagaa
     4201 agagtacatc gataaattaa tggtcgagcg tgaggctgaa gcgaaaaaag cccgccaatt
     4261 gaagaaaaag caggcttata aaccggatcc tgaggcgtca tttagctggt cagcaaatac
     4321 gtcgacgcgc ggcaggcgct gattaacgcc ccttcttttt gcgccccggc gaggtaaaac
     4381 gcttaccgtt ggacgccggg cgaccgcctt tttccgccgt tttttccatc ttaacgactg
     4441 gacgtttgat gcctaccgtt ttcggtttgg ccttcgcttt aggttttgcc tcggaagagg
     4501 aattttcaat gagcttaaag agatcgatta actcatcgtc ggttaaatcg cgccactcac
     4561 ccagcggaat gccgcttaag ctgacgttca taatgcgcgt acgttccagc tttttcactt
     4621 cgtaaccaaa gtgctcgcac atgcgacgaa tctgacggtt cagcccctgc accagggtaa
     4681 tgcggaagac aaacggtgct tcttttttga ctttgcactt tttagttacc gtcccgagga
     4741 tcggcactcc cgcccccata ccacgaataa attcatcggt aatcggttta tcaaccgtca
     4801 ccagatactc tttctcatga tcgttacccg cacggaggat cttattcact agatcgccgt
     4861 gattggtgag gaaaatcagc ccctgggaat ctttatccag gcggccaatc gggaacacgc
     4921 gtttgctgtg gttaacgaaa tcgacaatgt tatcgcgctc gccatcttcg gtggtgctga
     4981 caatgcctac gggcttgttc agggcgataa gtaccaaatc ttcggcttcc cgaggctcaa
     5041 tcaactgacc gtttactttc acaacgtcgc cggatttcac ctgatcgcca atggtggctc
     5101 gcttgccatt aaggaacaca ttgccttgct cgatatagcg atccgcttcg cggcgtgagc
     5161 aaattccgct ttcgctgatg tatttattta aacggactga tgagtcgggc agcataaatt
     5221 ctcctgtaaa acgcggaata taccgtacct cttagcgggg aaaaacaggg ttatgcggca
     5281 acgtcgaaat taaatgtgat ctgccacgcc ttttatcaca aaagtgtctc cggaagtacg
     5341 atgcaaattt gcctggcaaa ataaggataa tcaacaaatt acgaatcgct gcacacctgt
     5401 gctgatgact actacgcaga gaaacaaatg tgcaagagaa agcgctattt ccccccatgc
     5461 aaagagtgaa aaatcgcttt gaaaggccat catgatgggg ttatatcaga tcaagcggtg
     5521 agaaagcgta tttgcacaaa tgtgcagaac caggaggcgg caatggaaaa cagtgacgat
     5581 atccgtttga ttgtgaagat tgcccaactc tattacgaac aggatatgac gcaggcgcaa
     5641 attgcgcgcg aactgggtat ttaccgcacc accatcagcc gcttgcttaa acgaggccgc
     5701 gatcagggaa ttgtcaccat cgccatcaac tatgactaca acgaaaatct ctggctggag
     5761 cagcaagtga agcaaaagtt tggcctgaaa gacgttgtgg tggtgtcagg aaatgatgag
     5821 gatgaagata ctcaactggc gatgatgggg ttacacggcg cgcaactgct ggatcgcttg
     5881 ctggaacctg gcgatattgt cggtttttcc tggggccgcg cggtgagcgc actggttgaa
     5941 aacttgccgc aggcggggca atcgcggcag ttaatttgcg tgccgattat tggcggcccg
     6001 tccggtaaac tcgaaagccg ctatcacgta aacacattaa cctacagcgc ggcagcgaag
     6061 ctgaaagggg aatcgcatct cgcggatttt ccggctctgc tggataaccc attaattcgt
     6121 aatgggatca tgcagtctca gcactttaaa accatctctg cctactggga taatctggat
     6181 gtcgccctgg tgggaattgg ctcaccagcc attcgcgacg gcgctaactg gcatgcgttt
     6241 tatggtggtg aagagagtga cgacctgaat gcccgccagg ttgctggcga tatttgctcg
     6301 cgcttttttg atattcacgg cgcaatggtt gaaacgaata tgagcgaaaa aacactctct
     6361 atcgaaatga ataaattaaa gcaggcacgg tattccattg gcattgccat gagcgaagaa
     6421 aaatacagcg gaattgttgg tgcactgcgt ggaaaatata ttaattgtct ggtaacgaat
     6481 agcagcacag ctgaactatt actgaaataa aaatcttccc gatgtttacc gtaataaaaa
     6541 ttacggcggg tccttattat ctaaaatcag gagtctgtta tgcaaacgtg gttaaatttg
     6601 caggataaaa tcattattgt caccggtggc gcatccggca ttggtctggc gattgtggat
     6661 gaattattag cacaaggcgc gaatgtacag atggtcgata ttcacggtgg cgatggtcaa
     6721 tatgaaagcc ataaaggtta tcagttctgg ccgaccgata tttccagcgc caaagaggta
     6781 aatcatacgg tagcagaaat tatccagcgt tttggtcgca tcgacggtct ggttaacaac
     6841 gccggggtca atttcccgcg tctgcttgtc gatgagaaag cgcctgccgg gcagtatgaa
     6901 ctcaacgaag ctgcattcga aaaaatggtc aatatcaacc agaaaggcgt ttttctgatg
     6961 tcgcaggcgg tggcgcgaca gatggtcaaa caacatgatg gcgtgattgt gaatgtttcc
     7021 tcagaaagtg ggctggaagg ctcagaaggc caaagctgtt acgccgcgac caaagccgcg
     7081 ctcaatagct tcacgcgctc ctggtcgaaa gagctgggta agcacggtat ccgtgtggtc
     7141 ggtatcgcgc cggggattct ggaaaaaaca ggactgcgca cgccggaata tgaagaagcg
     7201 ctggcgtgga cgcgcaatat caccgtcgag cagctgcgtg aaggctatac caaaaacgcc
     7261 attcctattg ggcgcgccgg aagattagca gaagtggctg attttgtttg ttatctgctg
     7321 tctgaacgcg ccagctatat caccggagta accactaaca ttgcgggcgg caaaacgcgc
     7381 ggctaaggag gctttatggt caacgccatt ttttgtgcgc atggcagatt agcttgtgcc
     7441 atgctggaat cggttcagat ggtgtacggc gatgccaggg tcgaggcagt tgaatttgtg
     7501 cccggcgaga acgccggaga cattgtggca aagctggaga agttagtgag cattcacaat
     7561 catgacgagt ggctaatcgc cgtggattta cagtgcggca gcccgtggaa tgcggcagcg
     7621 atgcttgcga tgcgcaatcc tcgtctgcgg gtaatcagcg gcctttctct gccgctggcg
     7681 ctcgaactgg ttgataacca ggacagcatg aatgttgatg aactgtgtga acacctgacg
     7741 caaatcgcaa agcaaacctg cgtcgtctgg agacaggtgg caacagcgga ggaggatttc
     7801 tgatgaacat tacgctcgcc cgcattgatg accgcctgat ccacggccag gtcactaccg
     7861 tctggtctaa agtggctaac gcccagcgca ttattatctg caatgacgaa gtttataacg
     7921 atgaagttcg ccggacatta ttgcgtcagg ctgcgccacc gggaatgaag gttaacgtgg
     7981 tcaatattga aaaagccgtc gcggtttatc acaacccgca atatcaggac gaaaccgttt
     8041 tttatctttt cactcgccca caggatgcct tagcaatggt tcgccagggg gtaaaaatag
     8101 gtatcctgaa tattggcggt atggcctggc gtcctggtaa gaaacagtta accaaagcag
     8161 tttcattaga tgacgatgat attaacgcat ttcatgagtt aaataatctt ggcgtcatac
     8221 tggatttacg cgtcgtagcg tcagatccct caatcaatat tatcgacaaa ataaacgaac
     8281 agttaatcgc aaattaaata atagcgcctg tgtacatatg ctttaacagg cagggatgac
     8341 gacacttaaa aggtgacaaa tcatggaaat aagtacccta caaatcattg ctatatttct
     8401 tttttcctgt attgccggaa tgggcagcgt gctggatgaa tttcagactc atcgtccgtt
     8461 aattgcctgt acggtgattg gtttaattct cggtgattta aaaaccggaa ttatgctcgg
     8521 tggtacgctg gaattgatag ctctcggctg gatgaacgtc ggcgcggcgc aatctccaga
     8581 ttctgcactc gccagcataa tctccgccat tctggttatc gttggtcagc aaagcatcgc
     8641 caccggaatc gccatcgcgt tgcctgtggc tgctgcaggc caggtgctga cggtgtttgc
     8701 ccgtaccatc accgtagtgt tccagcacgc ggcggataaa gcagcggaag aagcacggtt
     8761 tcgcactctc gatattctgc atgtctccgc gcttggcgtg caggcactgc gcgttgctat
     8821 tccggcactg attgtctctc tgttcgtcag cgctgatatg gtgagcaata tgctgagcgc
     8881 cattcccgaa tttgtgaccc gtggactgca gattgctggc ggttttatcg tggtggtcgg
     8941 ttacgccatg gtgcttcgca tgatgggcgt gaaatatttg atgcctttct ttttcctcgg
     9001 tttcctcgca ggtggctacc tcgatctcag tctgctggcg ttcggtggcg tcggcgtgat
     9061 catggccctg ctctacatcc agttaaatcc acagtggcgt aaagctgaac cacatcccca
     9121 gaccaccact atcaccgccc ttgaccaact tgatgattaa cggagccatc atggaacaga
     9181 gaaaaattac acgcagcgat ctggtgagca tgtttctgcg ctccaacctg caacaggcat
     9241 cctttaactt tgaacgtatt cacgggctgg gtttttgcta cgacatgatc cccgccatca
     9301 aacgacttta cccattaaaa gaggatcagg ttgcggcgct caggcgacac ctggtgttct
     9361 tcaataccac gccagccgta tgtggcccgg tcatcggcgt cactgccgcc atggaagagg
     9421 cgcgagccaa cggcgcggaa attgatgacg gtaccattaa cggcatcaaa gtcggtctga
     9481 tgggaccatt ggcaggagtt ggcgatccac tggtctgggg aacgctgcgc ccgattaccg
     9541 ccgcgctcgg cgcatctctg gcactttcgg gcaacattct cggccccctg ctgttcttct
     9601 ttattttcaa cgcggtgcgt ctggcgatga agtggtatgg cctacagctc ggctttcgca
     9661 aaggggtgaa tatcgtcagc gatatgggcg gtaatttgct gcaaaaactc accgaaggcg
     9721 cgtcgattct cgggctgttt gtgatgggcg tgctggtgac caaatggacg tcaatcaacg
     9781 taccgttggt ggtttcacaa acgcctgccg ccgatggtgc caccgtcacc ataaccgtgc
     9841 agaacatcct cgaccaactt tgccctggtt tgctggcgct cggtctgacg ctgctgatgg
     9901 ttcgtctgct caacaaaaaa attaacccgg tatggctgat tttcgccctg tttggtttag
     9961 ggattatcgg taatgcgctg ggcttcctgt cctgattctt cgccccggcg caaccgtcgg
    10021 ggccatcgtt caacacgagg tggtttatga aaacaacagc tctgcgtctt tatggtaaac
    10081 gtgatttacg cctggaaacc tttgaccttc ctgaaatgca ggaggatgaa atcctcgcaa
    10141 cggtggtcac tgacagcctg tgcctctctt cctggaaaga ggccaatctg ggtgaaaatc
    10201 ataaaaaagt acccgacgat gtggcgacca atcccatcat catcggccac gaattttgcg
    10261 gcgatattct ggccgtgggt aaaaagtggc agcacaaatt ccagccgggt cagcgttatg
    10321 tgattcaggc caacctgcaa ctccccgacc gaccggattg ccccggctac tccttcccgt
    10381 gggtaggcgg cgaagcgacg catgtggtta ttcccaacga ggtcatggaa caagattgcc
    10441 tgctggcata cgacggcgaa acctattttg aaggctcgct ggttgaaccg ctttcctgcg
    10501 tgattggcgc gttcaacgcc aactatcatc ttcaggaagg tagttataac cacacgatgg
    10561 ggattcgccc gcaagggcgc acgctgatcc tcggcggcac cggaccaatg ggactgttgg
    10621 cgattgatta tgcgctacat ggacccgtta acccttcgct gctcgtcatt accgataccg
    10681 acaacgacaa attgagttat gcgcgcaagc actatccatc agaaccgcaa acactgattc
    10741 attatctcaa tgccgccgat gcagcatttg atacgttaat ggcactgagt ggcggtcacg
    10801 gcttcgatga tattttcgtc tttgtgccta atgaaggact ggtgactctc gcctcttcct
    10861 tgctggcgac agatggttgt ctgaatttct tcgccggacc gcaggataag cacttcagcg
    10921 cgccaattaa tttctacgat gtgcattatg catttaccca ctacgtgggc acgtcaggcg
    10981 gcaataccga cgacatgcgc gcagcggtca aattaattga agagaaaaaa gtgcaggccg
    11041 caaaagtggt aacacatatt cttgggctga atgccgcggg cgaaaccacg cttgaattgc
    11101 ctgccgtcgg cggcggcaaa aagctggtgt ataccgggaa atacctgccg ctgacgtcac
    11161 tcacgcagat tcaggatcaa gcactggcgg cgattctggc gcgtcatcag gggatctggt
    11221 ccggtgaggc ggagcaatac ctgctcgccc atgcagaggc aatttcccat gattaatcgc
    11281 gctacttaac tctgcatgtc gctggctggt cgccgcggca acttcgcgac ctgatgtgcg
    11341 gctgattgct gaagcggcac aatgtgcacg agaaggataa aaaaacatca gcgccttaag
    11401 gggcgctcaa attggtggca aagtgcacat tgtttatgcc ggatgcggaa tgaagcctta
    11461 ttcggcctac ataatcttga aaattcaata tattgcagtt atctggtagg ccaggtaagc
    11521 gtagcgcatc aggcaatcct gtgtttgtca tcagtctcag cgcctgtgca atatgtgaca
    11581 tcgtgagcca tgtcagtaag tggttaaatt cgcagtggta gcattccttc cgtgttttct
    11641 tactaaccgg agaagtaatg gaactgcttt tattgagtaa ctcgacgctg ccgggtaaag
    11701 cctggctgga acacgcgctg ccgctgattg ctgaacagtt gcagggtcgc cgttctgcgg
    11761 tatttattcc tttcgctggc gtaacgcaga cctgggatga ttacacagcg aaaacggctg
    11821 cggttctcgc tccgctgggt gtttctgtca ccggtattca tagcgttgtc gatcccgttg
    11881 ccgcgattga aaatgcggaa attgtgattg tcggcggcgg taatactttc cagttgctga
    11941 aacagtgccg cgagcgcggg ctgctggcac ccattactga cgtggtgaaa cgtggcgcgc
    12001 tgtatattgg ctggagcgca ggcgctaacc ttgcttgccc aaccattcgt accaccaacg
    12061 atatgccgat tatcgatccg caaggtttcg atgcgctaaa tctgttcccg ctacaaatca
    12121 acccgcactt caccaacgcg ctgccggaag gccataaagg tgaaacccgt gagcagcgta
    12181 ttcgcgaact gctggtcgtc gcgccagaac tgacgattat tggtctgccg gaaggtaact
    12241 ggatcacagt aagtaaaggt cacgctacgc tgggtggccc gaacaccact tatgtattta
    12301 aggctggtga agaagcggtg ccgctggaag cgggtcaccg tttttaagtg aattgccgga
    12361 tgcatgatcc ggcatcttgt tcatagtgcc tgatgcaacg ctttacgcgt cttatcaggc
    12421 cttcagggtc acggtattaa tactcgtccc gtccttcatc ttcatccggc tgttccagca
    12481 cactgtaagc caccgaacaa aacagcgagt tcagacgctg catatctccc agtaagccta
    12541 aatgtagcga actggtttca atactctgca cgttttgctg gtgcaggcga tcgacgtggg
    12601 cgtgggaata gcggcgatta agaatgcgaa aacgatgttt gctgcggcgc aaacgacggg
    12661 cgctggtaac atcgccagag aagaaaaccg acattgccag ttttaaatta ctgagcaatt
    12721 gctcatagag cgcatccagt tccttcaagc catcaagcga aaatgctcgc cttgctgcca
    12781 gcgatttgtc ggcaatttcg ctgcccatgc gctcgacgat atcggaggcc tgttcaaggt
    12841 tgagcgacat ttcgatgatc tccgcccagc ggcgcgactc ttcttctgcc agttcctctt
    12901 ttggcatccg cgccagatac agcttaatgg cggtatagag aacgttgatg tcatcagcca
    12961 gcttacgcag ctctttctcc tgccgtggct cgccgtgcat cactttattc agcccttcca
    13021 tcatctgttc catggcgtcg ccaatgcgca gggtttcgcg ggcagcgttc gccagagcaa
    13081 gcgtgggcgt atccagcgcg ctgacatcca gatgtttagg ccgtagctgg gtatccagtt
    13141 ccggttcatc gcgaataatc gttttgcaaa accgtgccat cgggtcaacg aatggcaaca
    13201 tgaccaggca gcgtacaagg ttgtagaaga cgtggaaata gatcaccaat tccgcttttg
    13261 gcaatggcaa cttccccatt gtctctgcca gcaaatgaac aaacggcagg ataatcaggc
    13321 tacccaccag cttaaacagc aggctaccca gcgcgacacg gcgggctgcg gcattggcgg
    13381 cactgttgtt gagcatcgcc agcaggccgg aaccgaggtt tgcaccaatc accagacaaa
    13441 gcgccactgg gaaagagata atgcctgcgg cggtcagagt cgcggtcagc agtaccgcgg
    13501 caaggctgga gtagctgata atggcgaaca tcgcaccaat cagcgcatcc agcaaaatgt
    13561 cgccagtcag cgaggcaaag atcacctgaa cgccgtttgc ctgagtgatc ggcgttacgg
    13621 cctgcacaat caactccagc gccagtaaaa tcagcccaag accaataccg acgcggccca
    13681 gttgcccggc gcgtgactgt ttgcgtccga ggaaaaaaat cacgccgata aaaataagta
    13741 acggtgacag ccaggataaa tcgaaggtga gaatacgcgc catcagcgcc gtcccgacat
    13801 ctgcacccag cacaatcacc agcgcaggcg cgagagcaac cagatcctga gcgacgaacg
    13861 aggtcaccag catggtggtg gcattactgc tctgcaccag tgcggtaacg ccgatccccg
    13921 cgcaaaaggc gagcggcttt ttttcgacgc tccggctaag gacagtacgc agacgcgcgc
    13981 cgaagacgcg cattacgcct gttcgaacaa tatgggtccc ccagaccagc agggcgacgg
    14041 cagaaagcag gtgaagcaaa gttagcacgg aatcaggttc tcctttttgt tatgcatttt
    14101 cctgttaaga tttttctaat cacgttgttt ttatccaggc atttctcttg tatgtacttt
    14161 cagtataaga gatttataca agaaagcatt taaaaaggca tttatataaa aatccacctg
    14221 tatctgttgc tatttctgtc acactttccc ctgtcgttta caccagataa tcgttgtcac
    14281 ttttgtgacc tctgagaaga taaacaacga aatatttaag accagatcct gtgaataaaa
    14341 ataattaagt aacaacggta ttacggagat tgaacagctt acggtagcaa aacgcataaa
    14401 ctcctgaatc gccaaaaaag agaaactaaa taatttttgt cttaaaaaac attgcgcaga
    14461 aaagaataaa gagaacaata atactatttg tagcgtaaca gtggcgactg cataagcgta
    14521 aaaaccgcct tcaacaccga tatccttaag taaagaaagt gaatgaacga tatcggaata
    14581 aaaagggata cgaccattac caagcgagta gaacagatat gtggctatcg cgataacatc
    14641 cataacaccc caaaaaagaa gaatgtattt tttatattga cgttgcataa agtaaatcct
    14701 ttttacgaaa acaaatatta ccaggaatta ttataattgc gggcgttttt ggattaacac
    14761 tccacccgta ttttttaaac ctctgtcagc tataccttca ctaaataaga catcgatacg
    14821 ccctttactt ttatcgtgat acagaatact gcatgagata caccttatac cctctttgga
    14881 tttacgaaaa tctaaccaat aacgaccatc
//
LOCUS       DAHHTG010000108        14818 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.109, whole genome shotgun sequence.
ACCESSION   DAHHTG010000108 DAHHTG010000000
VERSION     DAHHTG010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14818)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14818)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14818
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.109"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            405..1694
                     /gene="sdaC"
                     /locus_tag="NBI74_003412"
     CDS             405..1694
                     /gene="sdaC"
                     /locus_tag="NBI74_003412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease SdaC"
                     /protein_id="HCD1933694.1"
                     /translation="METTQTSTIASKDSRSAWRKTDTMWMLGLYGTAIGAGVLFLPIN
                     AGVGGMIPLIIMAILAFPMTFFAHRGLTRFVLSGKNPGEDITEVVEEHFGIGAGKLIT
                     LLYFFAIYPILLVYSVAITNTVESFMSHQLGMTPPPRAILSLILIVGMMTIVRFGEQM
                     IVKAMSILVFPFVGVLMLLALYLIPQWNGAALETLSLDTASATGNGLWMTLWLAIPVM
                     VFSFNHSPIISSFAVAKREEYGDMAEQKCSKILAFAHIMMVLTVMFFVFSCVLSLTPA
                     DLAAAKEQNISILSYLANHFNAPIIAWMAPIIAIIAITKSFLGHYLGAREGFNGMVIK
                     SLRGKGKSIEINKLNRITALFMLVTTWIVATLNPSILGMIETLGGPIIAMILFLMPMY
                     AIQKVPAMRKYSGHISNVFVVVMGLIAISAIFYSLFS"
     gene            1752..3119
                     /gene="sdaB"
                     /locus_tag="NBI74_003413"
     CDS             1752..3119
                     /gene="sdaB"
                     /locus_tag="NBI74_003413"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase II"
                     /protein_id="HCD1933695.1"
                     /translation="MISVFDIFKIGIGPSSSHTVGPMKAGKQFTDDLIARNLLKDVTR
                     VVVDVYGSLSLTGKGHHTDIAIIMGLAGNLPDTVDIDSIPGFIQDVNTHGRLMLANGQ
                     HEVEFPVDQCMNFHADNLSLHENGMRITALAGDKVVYSQTYYSIGGGFIVDEEHFGQQ
                     DSAPVEVPYPYSSAADLQKHCQETGLSLSGLMMKNELALHSKEELEQHLANVWEVMRG
                     GIERGISTEGVLPGKLRVPRRAAALRRMLVSQDKTTTDPMAVVDWINMFALAVNEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFIREVNANSLARYLLVASAIGSLYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGASPAQVCIAAEIAMEHNLGLTCDPVAGQVQVPC
                     IERNAIAAVKAVNAARMALRRTSEPRVCLDKVIETMYETGKDMNAKYRETSRGGLAMK
                     IVACD"
     gene            3231..3986
                     /gene="xni"
                     /locus_tag="NBI74_003414"
     CDS             3231..3986
                     /gene="xni"
                     /locus_tag="NBI74_003414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417278.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flap endonuclease Xni"
                     /protein_id="HCD1933696.1"
                     /translation="MAVHLLIVDALNLIRRIHAVQGSPCVETCQHALDQLIMHSQPTH
                     AVAVFDDENRSSGWRHQRLPDYKAGRPPMPEELHNEMPALRAAFEQRGVPCWSASGNE
                     ADDLAATLAVKVTQAGHQATIVSTDKGYCQLLSPTLRIRDYFQKRWLDAPFIDKEFGV
                     QPQQLPDYWGLAGISSSKVPGVAGIGPKSATQLLVEFQSLEGIYENLDAVAEKWRKKL
                     ETHKEMAFLCRDIARLQTDLHIDGNLQQLRLVR"
     gene            complement(4091..5239)
                     /gene="fucO"
                     /locus_tag="NBI74_003415"
     CDS             complement(4091..5239)
                     /gene="fucO"
                     /locus_tag="NBI74_003415"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HCD1933697.1"
                     /translation="MANRMILNETAWFGRGAVGALTDEVKRRGYQKALIVTDKTLVQC
                     GVVAKVTDKMDAAGLAWAIYDGVVPNPTITVVKEGLDVFQNSGADYLIAIGGGSPQDT
                     CKAIGIISNNPEFADVRSLEGLSPTNKPSVPILAIPTTAGTAAEVTINYVITDEEKRR
                     KFVCVDPHDIPQVAFIDADMMDGMPPALKAATGVDALTHAIEGYITRGAWALTDALHI
                     KAIEIIAGALRGSVAGDKDAGEEMALGQYVAGMGFSNVGLGLVHGMAHPLGAFYNTPH
                     GVANAILLPHVMRYNADFTGEKYRDIARVMGVKVEGMSLEEARNAAVEAVFALNRDVG
                     IPPHLRDVGVRKEDIPALAQAALDDVCTGGNPREATLEDIVELYHTAW"
     gene            complement(5267..5914)
                     /gene="fucA"
                     /locus_tag="NBI74_003416"
     CDS             complement(5267..5914)
                     /gene="fucA"
                     /locus_tag="NBI74_003416"
                     /EC_number="4.1.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculose-phosphate aldolase"
                     /protein_id="HCD1933698.1"
                     /translation="MERNKLARQIIDTCLEMTRLGLNQGTAGNVSVRYQDGMLITPTG
                     IPYEKLTESHIVFIDGNGKHEEGKLPSSEWRFHMAAYQSRPDANAVVHNHAVHCTAVS
                     ILNRPIPAIHYMIAAAGGNSIPCAPYATFGTRELSEHVALALKNRKATLLQHHGLIAC
                     EANLEKALWLAHEVEVLAQLYLTTLAITDPVPVLSDEEIAVVLEKFKTYGLRIEE"
     gene            6461..7777
                     /gene="fucP"
                     /locus_tag="NBI74_003417"
     CDS             6461..7777
                     /gene="fucP"
                     /locus_tag="NBI74_003417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose:H+ symporter permease"
                     /protein_id="HCD1933699.1"
                     /translation="MGNTSIQTQSYRAVDKDAGQSRSYIIPFALLCSLFFLWAVANNL
                     NDILLPQFQQAFTLTNFQAGLIQSAFYFGYFIIPIPAGILMKKLSYKAGIITGLFLYA
                     FGAALFWPAAEIMNYTLFLVGLFIIAAGLGCLETAANPFVTVLGPESSGHFRLNLAQT
                     FNSFGAIIAVVFGQSLILSNVPHQSQDVLDKMSPEQLSAYKHSLVLSVQTPYMIIVAI
                     VLLVALLIMLTKFPALQSDNHSDAKQGSFSASLSRLARIRHWRWAVLAQFCYVGAQTA
                     CWSYLIRYAVEEIPGMTAGFAANYLTGTMVCFFIGRFTGTWLISRFAPHKVLAAYALI
                     AMALCLISAFAGGHVGLIALTLCSAFMSIQYPTIFSLGIKNLGQDTKYGSSFIVMTII
                     GGGIVTPVMGFVSDAAGNIPTAELIPALCFAVIFIFARFRSQTATN"
     gene            7810..9585
                     /gene="fucI"
                     /locus_tag="NBI74_003418"
     CDS             7810..9585
                     /gene="fucI"
                     /locus_tag="NBI74_003418"
                     /EC_number="5.3.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose isomerase"
                     /protein_id="HCD1933700.1"
                     /translation="MKKISLPKIGIRPVIDGRRMGVRESLEEQTMNMAKATAALLTEK
                     LRHACGAAVECVISDTCIAGMAEAAACEEKFSSQNVGLTITVTPCWCYGSETIDMDPT
                     RPKAIWGFNGTERPGAVYLAAALAAHSQKGIPAFSIYGHDVQDADDTSIPADVEEKLL
                     RFARAGLAVASMKGKSYLSLGGVSMGIAGSIVDHNFFESWLGMKVQAVDMTELRRRID
                     QKIYDEAELEMALAWADKNFRYGEDENNKQYQRNAEQSRAVLRESLLMAMCIRDMMQG
                     NSKLADIGRVEESLGYNAIAAGFQGQRHWTDQYPNGDTAEAILNSSFDWNGVRKPFVV
                     ATENDSLNGVAMLMGHQLTGTAQVFADVRTYWSPEAIERVTGHKLDGLAEHGIIHLIN
                     SGSAALDGSCKQRDSEGKPTMKPHWEISQQEADACLAATEWCPAIHEYFRGGGYSSRF
                     LTEGGVPFTMTRVNIIKGLGPVLQIAEGWSVELPKDVHDILNKRTNSTWPTTWFAPRL
                     TGKGPFTDVYSVMANWGANHGVLTIGHVGADFITLASMLRIPVCMHNVEETKVYRPSA
                     WAAHGMDIEGQDYRACQNYGPLYKR"
     gene            9694..11112
                     /gene="fucK"
                     /locus_tag="NBI74_003419"
     CDS             9694..11112
                     /gene="fucK"
                     /locus_tag="NBI74_003419"
                     /EC_number="2.7.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculokinase"
                     /protein_id="HCD1933701.1"
                     /translation="MKQEVILVLDCGATNVRAIAVNRQGKIVARASTPNASDIAMENN
                     TWHQWSLDAILQRFADCCRQINSELTDCHIRGIAVTTFGVDGALVDKQGNLLYPIISW
                     KCPRTAVVMDNIERLISAQQLQAISGVGAFSFNTLYKLVWLKENHPQLLERAHAWLFI
                     SSLINHRLTGEFTTDITMAGTSQMLDIQQRDFSPQILQATGIPRRLFPRLVEAGEQIG
                     TLQNSAAAMLGLPVGIPVISAGHDTQFALFGAGAEQNEPVLSSGTWEILMVRSAQVDT
                     SLLSQYAGSTCELDSQAGLYNPGMQWLASGVLEWVRKLFWTAETPWQMLIEEARLIAP
                     GADGVKMQCDLLSCQNAGWQGVTLNTTRGHFYRAALEGLTTQLQRNLQMLEKIGHFKA
                     SELLLVGGGSRNTLWNQIKANMLDIPVKVLDDAETTVAGAALFGWYGVGEFNSPEEAR
                     AQIHYQYRYFYPQTEPEFIEEV"
     gene            11114..11536
                     /gene="fucU"
                     /locus_tag="NBI74_003420"
     CDS             11114..11536
                     /gene="fucU"
                     /locus_tag="NBI74_003420"
                     /EC_number="5.1.3.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose mutarotase"
                     /protein_id="HCD1933702.1"
                     /translation="MLKTISPLISPELLKVLAEMGHGDEIIFSDAHFPAHSMGPQVIR
                     ADGLLVSDLLQAIIPLFELDSYAPPLVMMAAVEGDTLDPEVERRYRNALSLQAPCPDI
                     IRINRFAFYERAQKAFAIVITGERAKYGNILLKKGVTP"
     gene            11594..12325
                     /gene="fucR"
                     /locus_tag="NBI74_003421"
     CDS             11594..12325
                     /gene="fucR"
                     /locus_tag="NBI74_003421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose operon activator"
                     /protein_id="HCD1933703.1"
                     /translation="MKAARQQAIVDLLLNHTSLTTEALSEQLKVSKETIRRDLNELQT
                     QGKILRNHGRAKYIHRQNQDSGDPFHIRLKSHYAHKADIAREALAWIEEGMVIALDAS
                     STCWYLARQLPDINIQVFTNSHPICHELGKRERIQLISSGGTLERKYGCYVNPSLISQ
                     LKSLEIDLFIFSCEGIDSSGALWDSNAINADYKSMLLKRAAQSLLLIDKSKFNRSGEA
                     RIGHLDEVTHIISDERQVTTSLVTA"
     gene            complement(12369..13469)
                     /gene="rlmM"
                     /locus_tag="NBI74_003422"
     CDS             complement(12369..13469)
                     /gene="rlmM"
                     /locus_tag="NBI74_003422"
                     /EC_number="2.1.1.186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010435097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytidine(2498)-2'-O)-methyltransferase
                     RlmM"
                     /protein_id="HCD1933704.1"
                     /translation="MNKVVLLCRPGFEKECAAEITDKAGQREIFGFARVKENAGYVIY
                     ECYQPDDGDKLICELPFSSLIFARQWFVVGELLQHLPPEDRITPIVGMLQGVVEKGGE
                     LRVEVADTNESKELLKFCRKFTVPLRAALRDAGVLANYETPKRPVVHVFFIAPGCCYT
                     GYSYSSNNSPFYMGIPRLKFPADAPSRSTLKLEEAFHVFIPADEWDERLANGMWAVDL
                     GACPGGWTYQLVKRNMWVYSVDNGPMAQSLMDTGQVTWLREDGFKFRPTRSNISWMVC
                     DMVEKPAKVAALMAQWLVNGWCRETIFNLKLPMKKRYEEVSHNLAYIQAQLDEHGINA
                     QIQARQLYHDREEVTVHVRRIWAAVGGRRDER"
     gene            complement(13462..13857)
                     /locus_tag="NBI74_003423"
     CDS             complement(13462..13857)
                     /locus_tag="NBI74_003423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HCD1933705.1"
                     /translation="MTSRFMLIFAAISGFIFVALGAFGAHVLSKTMGAVEMGWIQTGL
                     EYQAFHTLAILGLAVAMQRRISIWFYWSSVFLALGTVLFSGSLYCLALSHLRLWAFVT
                     PVGGVSFLAGWALMLVGAIRLKRKGVSHE"
     gene            complement(13876..14793)
                     /gene="gcvA"
                     /locus_tag="NBI74_003424"
     CDS             complement(13876..14793)
                     /gene="gcvA"
                     /locus_tag="NBI74_003424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     regulator GcvA"
                     /protein_id="HCD1933706.1"
                     /translation="MSKRLPPLNALRVFDAAARHLSFTRAAEELFVTQAAVSHQIKSL
                     EDFLGLKLFRRRNRSLLLTEEGQSYFLDIKEIFSQLTEATRKLQARSAKGALTVSLLP
                     SFAIHWLVPRLSSFNSAYPGIDVRIQAVDRQEDKLADDVDVAIFYGRGNWPGLRVEKL
                     YAEYLLPVCSPLLLTGEKPLKTPEDLAKHTLLHDASRRDWQTYTRQLGLNHINVQQGP
                     IFSHSAMVLQAAIHGQGVALANNVMAQSEIEAGRLVCPFNDVLVSKNAFYLVCHDSQA
                     ELGKIAAFRQWILAKAAAEQEKFRFRYEQ"
BASE COUNT         3632 a         3898 c         3631 g         3657 t
ORIGIN      
        1 caaacgtttc cctattttca taaaccacat tttatcgcca tgaaaaagaa attatcgcca
       61 tgattaacta aaagtattga tttttccagt tcaacctaca tatattgcgc gccccggaag
      121 aagtcagatg tcgtttaatg ggcaaatatt gcccttaaat tctcttttac ttttgattta
      181 cagagtaaag cgttgggatt atctatcttc caagtagatt attgtatttg ggatcaagat
      241 cactgataga tacataactt gtgtgtatct ttccgccctc aaattattac ggtggaaaat
      301 gattaagcca tcgccgatag acagatttca tttttacggt caggcacctt cccgggctga
      361 actggctaaa agctgaatta tttgcattcc tccaggagaa atagatggaa acgactcaaa
      421 ccagcacgat tgcgtcgaaa gactctcgta gtgcctggcg caagacagac accatgtgga
      481 tgctgggcct ttacggcacg gcaatcggcg cgggcgtgct gttcctgcca atcaacgccg
      541 gtgttggcgg tatgatcccg ctgatcatca tggctatcct tgcattcccg atgacgtttt
      601 ttgctcaccg cggcctgact cgcttcgtac tgtctggtaa aaacccgggc gaagacatca
      661 ccgaggttgt agaagaacac tttggtattg gcgcaggtaa actgattact ctgctctact
      721 tcttcgctat ctacccgatc ctgctggttt atagcgtggc aatcaccaat acagttgaaa
      781 gcttcatgtc tcaccaactg ggtatgacgc caccgccacg tgcgattctg tcgctgatcc
      841 tgatcgtggg tatgatgacc atcgttcgct tcggtgagca gatgatcgtt aaagcgatga
      901 gtattctggt attcccgttt gttggcgtac tgatgctgct ggctctgtac ctgatcccgc
      961 agtggaacgg cgcagcactg gaaacgctgt ctctggacac tgcatctgca accggaaacg
     1021 gtctgtggat gaccctgtgg ctggcaattc cggtaatggt gttctcgttc aaccactctc
     1081 cgatcatctc ttctttcgcc gttgcgaagc gtgaagagta cggcgatatg gcagaacaga
     1141 aatgctctaa gatcctggca ttcgcacaca tcatgatggt gctgaccgta atgttcttcg
     1201 tcttcagctg cgtactgagc ctgactccgg cagacctggc tgcggctaaa gagcagaaca
     1261 tctcgattct gtcttacctg gctaaccact ttaacgcgcc gatcatcgcg tggatggctc
     1321 cgattatcgc gattatcgct atcaccaaat ccttcctcgg ccactacctg ggcgcacgtg
     1381 aaggcttcaa cggtatggtg attaaatctc tgcgtggtaa aggtaagtct atcgaaatca
     1441 acaagctgaa ccgtatcact gcgctgttca tgctggtaac gacctggatt gttgccaccc
     1501 tgaacccgag catcctgggt atgattgaaa ccctgggcgg cccaatcatc gcgatgatcc
     1561 tgttcctgat gccgatgtac gcaattcaga aagtaccggc aatgcgtaag tacagcggtc
     1621 acatcagcaa cgtattcgtt gtcgtgatgg gtctgattgc aatctccgca atcttctact
     1681 ctctgttcag ctaagtcctt tcgcgccgct ttcgggcggc gcttcctccg ttttaacgcg
     1741 atgtatttcc tatgattagc gtattcgata ttttcaaaat cggcattggc ccttccagtt
     1801 ctcataccgt tggaccaatg aaagcgggta aacaatttac cgacgatctg attgcccgta
     1861 acctgcttaa agacgtgacc cgcgtggtgg ttgacgtgta cggctcgctc tctctgaccg
     1921 gtaaaggcca ccacacagat atcgccataa ttatgggtct ggcgggtaac ctgccggata
     1981 ccgtggatat cgattccatc cctggtttta ttcaggatgt gaatactcat ggtcgcctga
     2041 tgctggcaaa cggtcagcat gaagtggagt tcccggttga tcagtgcatg aacttccatg
     2101 ccgacaacct ttctctgcat gaaaacggta tgcgcattac cgcgctggcg ggcgataaag
     2161 tcgtttacag ccagacttac tactctattg gcggtggctt tatcgttgat gaagagcatt
     2221 ttggtcagca ggatagcgca ccggttgaag ttccttatcc gtatagttcc gctgccgatc
     2281 tacaaaaaca ttgtcaggaa accgggctgt cactctctgg cctgatgatg aaaaacgagc
     2341 tggcgctgca cagcaaagaa gagctggaac agcacctggc gaacgtctgg gaagtcatgc
     2401 gtggcggtat tgaacgcggt atttctaccg aaggcgtgtt acctggcaaa ctgcgcgttc
     2461 cacgccgtgc tgcggcacta cgccggatgc tggtcagcca ggataaaacc accactgacc
     2521 cgatggcggt agttgactgg atcaacatgt ttgcactggc agtgaacgaa gagaacgctg
     2581 ctggcggtcg cgtggtgact gcgccgacta acggtgcgtg cgggattatc ccggccgtgc
     2641 tggcgtacta cgacaagttt atccgcgaag tgaacgctaa ctcactggcg cgttacctgc
     2701 tggtagccag cgccattggt tctctttata agatgaacgc ctcgatttct ggtgccgaag
     2761 tcggctgcca gggtgaagtc ggcgtggcgt gctctatggc ggcagctggt ctggcagaac
     2821 tgttaggcgc aagcccggcg caggtgtgca tcgcggcgga aatcgccatg gagcataacc
     2881 tcggtctgac gtgtgacccg gttgctggac aggtgcaggt accgtgcatc gagcgtaacg
     2941 ccattgcggc agtaaaagcg gtgaacgccg cgcgtatggc gctgcgccgt accagcgagc
     3001 cgcgcgtctg cctcgataaa gttatcgaga ccatgtacga aacaggtaaa gatatgaacg
     3061 ccaagtaccg cgaaacctct cgcggcggcc tggcaatgaa gatcgttgcc tgcgattaat
     3121 cgctctccag aggcctcttt ttgcgaggcc tcttcccgat ttctcatcca gccatagcct
     3181 gttctggcat cgaatgttac ccttatcgcc tgatctttaa gggggttatc gtggctgttc
     3241 atttgcttat tgtcgatgca ctgaatctta ttcgtcgcat tcatgccgtt cagggttcgc
     3301 cctgtgtcga aacctgccaa cacgcgctcg atcagctcat tatgcacagc cagccaaccc
     3361 acgcggtcgc cgtttttgat gatgaaaacc gcagtagcgg ctggcgtcat cagcgtttac
     3421 cagattacaa agcgggtcga ccgccaatgc cggaagagtt gcacaacgag atgcctgcat
     3481 tacgcgccgc ctttgagcaa cgcggcgtcc cgtgctggtc cgccagcggc aacgaagccg
     3541 atgacttagc cgccacgctg gcggtcaaag tgacacaggc cgggcatcag gcaacgattg
     3601 tttcgacaga taaaggctac tgtcagttac tttcaccgac attacgtatt cgtgattact
     3661 tccagaaacg ttggctggat gcgccattta tcgataaaga atttggcgtt caaccgcagc
     3721 agttgcccga ttactggggg cttgcgggga tcagcagttc aaaggtaccg ggtgttgcgg
     3781 ggatcggacc aaaaagcgcc acgcagctac tggtcgagtt tcagagtctg gaagggatat
     3841 atgagaatct ggatgcggtt gccgaaaagt ggcgcaaaaa attagaaacc cataaagaga
     3901 tggcgtttct gtgccgcgat attgcccgct tacaaaccga tttgcatatc gacggcaatt
     3961 tacagcaatt gcggttggta aggtaacggc gtgccggatg caaacgatta tcaacacatt
     4021 gaatttaatg cacttatatg ccggataaga cgcggctagc gtcgcatccg gcattatcac
     4081 atcagcacat ttaccaggcg gtatggtaaa gctctacaat atcctcaagc gttgcttcac
     4141 gcgggttgcc accggtacaa acatcatcca gtgccgcctg cgccagtgcc ggaatgtctt
     4201 ccttgcgtac accaacatca cgtaaatgtg gcggaatacc gacatcacgg ttgagagcaa
     4261 acaccgcttc aacagcggca ttacgcgcct cttccaggct cataccttcc actttcacgc
     4321 ccataacgcg cgcgatatcg cggtacttct caccggtaaa gtcagcgtta tagcgcatga
     4381 catgcggtaa caggatggca tttgcaacac cgtgtggcgt gttgtaaaac gcgcccagtg
     4441 gatgcgccat accatgcacc aaccctaacc caacattcga gaatcccata cccgcaacat
     4501 actgcccgag cgccatttct tctccggcat ccttatcacc agcaaccgat cctcgcagcg
     4561 ccccagcaat gatttcaatc gctttaatgt gcagcgcatc ggttagcgcc cacgcgccac
     4621 gggtaatata cccctcaata gcatgagtga gcgcatcgac acccgtcgcg gctttcagcg
     4681 ctggaggcat accatccatc atgtcagcgt caataaacgc cacctgcggg atatcatgcg
     4741 gatcaacgca aacaaacttg cgccgtttct cttcgtcagt gatcacgtag ttaatggtca
     4801 cttctgccgc agtgcctgct gtggtgggga ttgccagaat cggtacactg ggtttattgg
     4861 tcggggaaag cccttccagg ctacgcacat cggcaaactc cgggttgttg ctgataatgc
     4921 caatcgcttt acacgtatcc tgtggagaac caccaccaat agcgatcagg taatccgcgc
     4981 cgctattctg gaatacatcg agcccttctt tgacgacagt aattgttggg ttgggcacga
     5041 cgccgtcgta aatcgcccat gccagtcctg cggcatccat cttgtcggtc actttcgcca
     5101 ccacgccgca ttgcaccagc gttttatcgg tgacgatcag cgccttctga taaccacggc
     5161 gtttcacttc atcggttaaa gctccaacag cacctcgacc aaaccatgcc gtttcgttca
     5221 gaatcattct gttagccatc atccttctcc ttgttgcttt acgaaattac tcttcaatcc
     5281 gtaacccgta ggttttgaat ttctccagca ctacggcaat ctcttcatcg ctcagcactg
     5341 gcaccgggtc cgtaatcgcc agggtcgtca ggtaaagttg tgccagcact tcaacttcat
     5401 gcgccagcca taacgctttc tccagattcg cctcacaagc gataagccca tgatgttgta
     5461 acaaagttgc cttacgattt ttgagagcca gcgcaacatg ttcagaaagt tcgcgtgttc
     5521 caaaggtcgc ataaggcgcg caaggaatag aattaccgcc agccgccgca atcatgtagt
     5581 gaatcgcggg gatcggtcgg ttaagaatgg aaactgccgt acaatgaacg gcatgattgt
     5641 gaacaaccgc gttggcatcc ggtctgcttt gataggccgc catatggaaa cgccactcgc
     5701 ttgaggggag ttttccttcc tcatgtttgc cgttgccatc aataaagaca atatgcgact
     5761 ccgtcagttt ttcatatgga atacctgtag gcgtaatcag catcccatcc tgataacgta
     5821 cactgacgtt acccgctgtt ccctggttca gccccaggcg agtcatttcc aggcaagtgt
     5881 caataatctg acgagcaagt ttatttcgtt ccattagcta cctctctctg attcaaaaca
     5941 gggcaataat gttgttcctt tcacactatt gaattagccg tttaattact caccatattc
     6001 ttcctgatta accagaaaga aattcacaag cttatatttt gtgacctggt tcaactaatc
     6061 acagtaaata actgcaagtt ctctttttat aaccccatta aaaatgaccg ctcttaaaaa
     6121 tatttatcaa aacggtcatt tttctactcc tccctgcgag gaatgcccgt tttcggcaca
     6181 aacaattaat acggtcatct ggtttgtgtt ttttatgatt tattttctga aacgggcatg
     6241 aaatttcgat tattaaagtg atggtagtca cataaagtca ccctccggct aataagtgtg
     6301 accgccgtca tattacagag cgttttttat ttgaaaatga atccatgagt tcatttcaga
     6361 caggcaaata ttcactgata tgaagcccga actcgctggt tttgcacttt tgaaaacata
     6421 accgattacg tgcttaagct tctgaaccta agaggatgct atgggaaaca catcaataca
     6481 aacgcagagt taccgtgcgg tagataaaga tgcagggcaa agcagaagtt acattattcc
     6541 attcgcgctg ctgtgctcac tgttttttct ttgggcggta gccaataacc ttaacgacat
     6601 tttattgcct caattccagc aggcttttac gctgacaaat ttccaggctg gcctgatcca
     6661 atcggccttt tactttggtt atttcattat cccaatccct gccgggatat tgatgaaaaa
     6721 actcagttat aaagcaggga ttattaccgg attgttttta tatgccttcg gcgctgcatt
     6781 attctggccc gccgcagaaa taatgaacta caccttgttt ttagttggcc tatttattat
     6841 tgcagccgga ttaggttgtc tggaaactgc cgcaaaccct tttgttacgg tattagggcc
     6901 ggaaagcagt ggtcacttcc gcttaaatct tgcgcaaaca tttaactcat ttggcgcaat
     6961 tatcgcggtt gtctttgggc aaagtcttat tttgtctaac gtgccacatc aatcgcaaga
     7021 cgttctcgat aaaatgtctc cagagcaatt gagtgcgtat aaacacagcc tggtattatc
     7081 ggtacagaca ccttatatga tcatcgtggc tatcgtgtta ctggtcgccc tgctgatcat
     7141 gctgacgaaa ttcccggcat tgcagagtga taatcacagt gacgccaaac aaggatcgtt
     7201 ctccgcatcg ctttctcgcc tggcgcgtat tcgccactgg cgctgggcag tattagcgca
     7261 attctgctac gttggcgcac aaacggcctg ctggagctat ttaattcgct acgctgtaga
     7321 agaaattcca ggtatgacag caggctttgc cgctaactat ttaaccggaa ccatggtgtg
     7381 cttctttatt ggtcgtttca ccggtacctg gctcatcagt cgcttcgcgc cacacaaagt
     7441 cctggcagcc tacgcattaa tcgctatggc actgtgcctg atctcagcct tcgctggcgg
     7501 tcatgtgggc ttaatagccc tgactttatg cagtgccttt atgtcgattc agtacccaac
     7561 aatcttctcg ctgggcatta agaatctcgg ccaggacacc aaatacggtt cgtccttcat
     7621 cgttatgacc attattggcg gcggtattgt cactccggtc atggggtttg tcagtgacgc
     7681 agcgggcaac atccccactg ccgaactgat cccggcactc tgcttcgcag tcatctttat
     7741 ctttgcccgt ttccgttctc aaacggcaac taactgaaca tattttccga ataaagtgag
     7801 gaatcagtaa tgaaaaaaat cagcttaccg aaaattggta tccgcccggt tattgacggt
     7861 cgtcgcatgg gtgttcgtga gtcgcttgaa gaacaaacaa tgaatatggc gaaagccacg
     7921 gctgcactgc tgaccgagaa actgcgccat gcctgcggag ctgccgtcga gtgtgtcatt
     7981 tccgatacct gtatcgcggg tatggctgaa gccgctgctt gcgaagaaaa attcagcagt
     8041 cagaatgtag gcctcaccat tacggtaacc ccttgctggt gctatggcag tgaaaccatc
     8101 gacatggatc caacccgccc gaaagccatt tggggcttta acggcactga acgtcccggc
     8161 gctgtttacc tggcagcggc tctggcagct cacagccaga aaggcatccc ggcattctcc
     8221 atttacggtc atgacgttca ggatgccgat gacacatcga ttcctgccga tgttgaagaa
     8281 aaactgctgc gctttgcccg cgccggttta gcagtcgcca gcatgaaagg taaaagttat
     8341 ctgtcgctgg gcggcgtttc gatgggtatc gccggttcca tcgttgatca caacttcttt
     8401 gaatcctggc tgggaatgaa agtccaggcg gtggatatga ccgaactgcg tcgccgtatc
     8461 gatcagaaga tttacgacga agccgaactg gaaatggcgc tggcctgggc tgataaaaac
     8521 ttccgctatg gcgaagatga aaataacaaa cagtatcaac gcaatgccga gcaaagccgc
     8581 gcagttctgc gcgaaagttt actgatggcg atgtgtatcc gcgacatgat gcaaggcaat
     8641 agcaaactgg ccgatattgg tcgcgtggaa gaatcacttg gctacaacgc catcgctgca
     8701 ggcttccagg ggcaacgtca ctggaccgat caatatccca atggcgatac cgctgaagcg
     8761 atcctcaaca gttcatttga ctggaatggc gtgcgcaaac cgtttgtcgt ggcgaccgaa
     8821 aacgacagtc ttaacggcgt ggcaatgtta atgggccacc agctcaccgg caccgctcag
     8881 gtatttgccg atgtccgtac ctactggtca ccagaagcaa ttgagcgtgt aacggggcat
     8941 aaactggacg ggctggcaga acacggcatc atccatttga tcaactccgg ttctgctgcg
     9001 ctggacggct cctgtaaaca acgcgacagc gaaggtaaac cgacgatgaa gccgcactgg
     9061 gaaatctctc agcaagaggc tgacgcttgc ctcgccgcta ccgaatggtg cccggcgatc
     9121 cacgaatact tccgtggcgg cggttactct tcccgcttcc ttaccgaagg cggcgtcccg
     9181 ttcaccatga ctcgtgtcaa catcatcaaa ggcctgggac cggtactgca aatcgcggaa
     9241 ggctggagcg tggaattgcc gaaggatgtg catgacatcc tcaacaaacg caccaactca
     9301 acctggccaa ccacctggtt tgcaccgcgc ctcaccggta aagggccgtt tacggatgtg
     9361 tactcggtaa tggcgaactg gggcgctaac cacggagttc tgaccatcgg ccacgttggc
     9421 gcagacttta tcactcttgc ctccatgctg cgtatcccgg tatgtatgca caacgttgaa
     9481 gagaccaaag tgtatcgtcc ttctgcctgg gccgcgcacg gcatggatat tgaaggccag
     9541 gattaccgcg cttgccagaa ctacggcccg ttgtacaagc gttaatacat gactccctga
     9601 tgtgatgccc ggtcgctccg gctaccgggc ctgaacaagc aagagtggtt agccggataa
     9661 gcaatgttat ccggctatat tgcaggagcg attatgaaac aagaagttat cctggtactc
     9721 gactgtggcg cgaccaatgt cagggccatc gcggttaatc ggcagggaaa aattgttgcc
     9781 cgcgcctcaa cgcctaatgc cagcgatatc gcgatggaaa acaacacctg gcaccagtgg
     9841 tctttagacg ccattttgca acgctttgct gattgctgtc ggcaaatcaa tagtgaactg
     9901 actgattgcc acatccgtgg tatcgccgtc accacctttg gtgtggatgg cgctctggta
     9961 gataagcaag gcaatctgct ctatccgatc attagctgga aatgtccgcg aacagccgtg
    10021 gtaatggaca atattgaacg gttgatctcc gcacagcagt tgcaggctat ttctggagtc
    10081 ggagccttta gtttcaatac gttatataag ttggtgtggt tgaaagaaaa tcatccacaa
    10141 ctgctggaac gcgcgcacgc ctggctcttt atttcgtcgc tgattaacca ccgtttaacc
    10201 ggcgaattca ctactgatat tacgatggcc ggaaccagcc agatgctgga tatccagcaa
    10261 cgcgatttca gtccgcaaat tttacaagcc accggtattc cacgccgact cttccctcgt
    10321 ctggtggaag cgggtgaaca gattggtacg ctacagaaca gcgccgcagc aatgctcggc
    10381 ttacccgttg gcataccggt gatttccgca ggtcacgata cccagttcgc cctttttggc
    10441 gctggtgccg aacaaaatga acccgtgctc tcttccggta catgggaaat tttaatggtc
    10501 cgcagcgccc aggttgatac ttcgctgtta agtcagtacg ccggttctac ctgcgaactg
    10561 gatagccagg cagggttgta taacccaggt atgcaatggc tggcatccgg cgtgctggaa
    10621 tgggtgagaa aactgttctg gacggctgaa actccctggc aaatgttgat tgaagaagct
    10681 cgcctgatcg cgcctggcgc agatggagtg aaaatgcagt gtgatttatt gtcgtgccag
    10741 aacgctggct ggcaaggagt gacgcttaat accacgcggg ggcatttcta tcgcgcggcg
    10801 ctggaagggt taaccacgca attacagcgc aatctacaga tgctggaaaa aatcgggcac
    10861 tttaaagcct ctgaattatt gttagtcggc ggaggaagtc gcaacacatt gtggaatcag
    10921 attaaagcca atatgcttga tattccggta aaagttctcg acgatgccga aacgaccgtc
    10981 gcaggagctg cgctgtttgg ttggtatggc gtaggggaat ttaacagccc ggaagaagcc
    11041 cgcgcgcaga ttcattatca gtaccgttat ttctacccgc aaactgaacc tgaatttata
    11101 gaggaggtgt gaaatgctga aaacaatttc gccgttaatt tctcccgaac tattgaaagt
    11161 gctggcagag atggggcatg gagatgaaat tattttttcc gatgctcact ttcccgccca
    11221 ttcgatggga ccgcaggtga tccgcgctga tggcctgttg gtgagcgact tgctccaggc
    11281 gattatcccg ttatttgaac tggacagtta tgcaccaccg ctggtgatga tggcggcggt
    11341 agaaggtgac actctcgatc ctgaagtaga acgacgttat cgtaatgcgc tttcactaca
    11401 agccccctgt cctgacatca tccgcatcaa tcgttttgcg ttttatgaac gggcgcaaaa
    11461 agcctttgcg atcgttatca caggcgaacg agcgaagtac gggaatattc ttttaaaaaa
    11521 aggggtaaca ccgtaatctc ataccggtac gcccgcatga cgcgggcggt tatcgaatga
    11581 tggggtgaaa aatatgaaag cggcacgcca gcaagcgata gtcgacctgc tgctgaacca
    11641 taccagcctg accacggaag ctctctctga acagctaaag gtcagtaaag aaaccattcg
    11701 tcgcgatctc aatgaattac agacgcaggg taaaattctg cgcaatcatg ggcgcgctaa
    11761 atatatccac cgtcaaaatc aagacagtgg cgatcccttt cacatccggc tgaaaagcca
    11821 ttatgcgcat aaagcagata tcgcgcgcga ggcgctcgcg tggattgaag aagggatggt
    11881 gatagcctta gacgccagtt caacctgctg gtatctggca cgccagttgc ctgacatcaa
    11941 catccaggtc ttcaccaata gccatccgat ttgccatgaa ctcggtaaac gcgaacgcat
    12001 tcaactgatc agttccggcg gcacacttga gcgcaaatat ggctgttacg tcaatccctc
    12061 gctgatttcc caacttaaat cgctggaaat cgatctgttt attttttctt gtgaagggat
    12121 cgatagcagc ggcgcactgt gggactccaa tgccatcaac gctgattaca aatcaatgtt
    12181 attaaaacgt gccgcgcaat cgttgttatt gattgataaa agtaaattta atcgttcagg
    12241 ggaagcccgc atcgggcatc tggatgaggt aacgcacatt atttctgatg agcgccaggt
    12301 tacaacttct ttggtaacag cctgacggaa agggtagcag gccggagacg acgccccggc
    12361 cttacctgtt atcgctcgtc gcgacgacca cccaccgcag cccagatacg gcggacgtgc
    12421 accgtcactt cttcgcgatc gtgatacaac tgccgcgcct gaatctgagc atttatgcca
    12481 tgttcatcaa gctgtgcctg aatatacgcc agattgtgtg acacttcttc gtagcgtttt
    12541 ttcatcggca gtttgagatt gaagatagtt tcacggcacc agccattaac cagccactgc
    12601 gccattaatg ccgcaacttt cgccggtttt tcaaccatat cgcataccat ccaggagata
    12661 ttgctgcgtg tcggacggaa tttgaaaccg tcttcccgca gccacgtcac ctgcccggta
    12721 tccatcagac tttgcgccat cgggccgttg tcgacggaat aaacccacat gttgcgcttc
    12781 accagttggt aggtccagcc gcctgggcaa gcacctaaat ccaccgccca catcccgttc
    12841 gccaggcgtt catcccactc atccgcggga ataaacacat gaaatgcctc ttccagtttg
    12901 agcgtggaac gactcggcgc atctgccgga aatttcaggc gcggaatgcc catatagaac
    12961 ggcgaattat tgctgctgta tgagtaacca gtatagcaac agcctggtgc aataaagaat
    13021 acatgcacaa ccggacgctt cggcgtttca tagttcgcca gcacccccgc atcacgcaag
    13081 gcagcgcgta gcggcacggt gaatttacgg cagaatttca gcaactcttt gctttcgttg
    13141 gtatcggcaa cttcaacacg cagttcaccg cccttctcta ctacaccctg caacatgcca
    13201 acaatggggg taatacgatc ttctggcggc aaatgctgca aaagctcccc gactacaaac
    13261 cactggcggg caaaaattaa tgaactgaac ggcagctcac agattaactt atcgccatca
    13321 tcaggttgat aacattcata aatcacataa cccgcattct ctttcacgcg ggcaaaaccg
    13381 aaaatttccc gctggccggc tttatcggta atttctgcgg cgcactcttt ttcaaagccc
    13441 ggacggcaca gcaatacaac cttattcatg acttacgccc ttgcgcttta aacggatagc
    13501 accaactaac attaacgccc agcccgcaag gaagctcacg ccgccaaccg gtgtgacaaa
    13561 cgcccacaaa cgcagatggg acagcgccag gcaataaagg ctgccgctga acagcaccgt
    13621 gcctaacgcg aggaaaacgc tactccagta aaaccagata ctgatgcgac gctgcattgc
    13681 caccgccaga cctaagatcg ccagcgtatg aaacgcctgg tattcgaggc cggtctggat
    13741 ccagcccatc tcaacggccc ccatggtttt acttaacaca tgcgcgccaa aagcgcccag
    13801 agctacaaaa atgaagccgc taatggcggc gaaaatcagc ataaaacggc tggtcatggt
    13861 cgtaccctac gtaaattatt gttcataacg aaagcggaat ttttcttgtt cagcagcggc
    13921 tttcgccagg atccattggc gaaaggcggc tattttaccc agttctgcct ggctgtcatg
    13981 acaaaccaga taaaaagcgt ttttactgac cagaacatca ttaaacgggc aaacaagacg
    14041 tccggcctcg atttcagatt gcgccatcac gttatttgcc agcgccactc cctgcccgtg
    14101 gatagccgct tgcagcacca tggcgctatg gctaaaaatt ggcccttgct gaacgttgat
    14161 atgatttaac cccaactgtc gggtatatgt ctgccagtca cggcgtgacg catcatgtaa
    14221 taacgtatgt ttagccagat cttccggggt cttcaagggt ttttcgccag tcagcagtag
    14281 cggcgagcac accggcaata aatattcggc gtacagtttt tccacccgta gccccggcca
    14341 gttgccccga ccataaaata tcgccacatc aacatcatcc gccagcttat cttcctgacg
    14401 atcaaccgcc tggattcgaa cgtcaattcc cggataagct gaattaaagc tggaaagtcg
    14461 cggaaccaac caatgaatcg cgaaactggg gagtaaactg accgtcaacg cccccttagc
    14521 gctacgggcc tggagtttac gcgtcgcttc ggttaattgc gaaaatatct ctttgatatc
    14581 gaggaaatag ctttgccctt cctcggtcag caggagtgaa cgattacggc ggcggaacag
    14641 ttttagcccc agaaaatcct caagagactt gatttgatga cttactgcgg cctgggtcac
    14701 aaaaagctct tctgctgcgc gagtgaaact taaatggcgt gctgcggcat caaaaactcg
    14761 taaggcattt agcggtggta atcgtttaga catggctttt aaactttgat gttaaatg
//
LOCUS       DAHHTG010000109        14519 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.110, whole genome shotgun sequence.
ACCESSION   DAHHTG010000109 DAHHTG010000000
VERSION     DAHHTG010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14519)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14519)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14519
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.110"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            complement(26..1480)
                     /gene="xylB"
                     /locus_tag="NBI74_003425"
     CDS             complement(26..1480)
                     /gene="xylB"
                     /locus_tag="NBI74_003425"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HCD1933707.1"
                     /translation="MYIGIDLGTSGVKVILLNEQGEVVAAQTEKLTVSRPHPLWSEQD
                     PEQWWQATDRAMKALGVQHSLQDVKALGIAGQMHGATLLDAQQRVLRPAILWNDGRCA
                     QECTLLEARVPQSRVITGNLMMPGFTAPKLLWVQRHEPEIFRQIDKVLLPKDYLRLRM
                     TGEFASDMSDAAGTMWLDVAKRDWSDDMLQACHLSRDQMPALYEGSEITGALLPEVAK
                     AWGMAAVPVVAGGGDNAAGAVGVGMVDANQAMLSLGTSGVYFAVSEGFLSKPESAVHS
                     FCHALPQRWHLMSVMLSAASCLDWAAKLTGLSNVPALITAARQADESAEPVWFLPYLS
                     GERTPHNNPQAKGVFFGLTHQHGPNELARAVLEGVGYALADGMDVVHACGIKPQSVTL
                     IGGGARSEYWRQMLADISGQQLDYRTGGDVGPALGAARLAQIAANPEKSLIELLPQLP
                     LEQSHLPDAQRYAAYQPRRETFRRLYQQLLPLMA"
     gene            complement(1551..2873)
                     /gene="xylA"
                     /locus_tag="NBI74_003426"
     CDS             complement(1551..2873)
                     /gene="xylA"
                     /locus_tag="NBI74_003426"
                     /EC_number="5.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
                     /protein_id="HCD1933708.1"
                     /translation="MQAYFDQLDRVRYEGSKSSNPLAFRHYNPDELVLGKRMEEHLRF
                     AACYWHTFCWNGADMFGVGAFNRPWQQPGEALALAKRKADVAFEFFHKLHVPFYCFHD
                     VDVSPEGASLKEYINNFAQMVDVLAGKQEESGVKLLWGTANCFTNPRYGAGAATNPDP
                     EVFSWAATQVVTAMEATHKLGGENYVLWGGREGYETLLNTDLRQEREQLGRFMQMVVE
                     HKHKIGFQGTLLIEPKPQEPTKHQYDYDAATVYGFLKQFGLEKEIKLNIEANHATLAG
                     HSFHHEIATAIALGLFGSVDANRGDAQLGWDTDQFPNSVEENALVMYEILKAGGFTTG
                     GLNFDAKVRRQSTDKYDLFYGHIGAMDTMALALKIAARMIEDGELDKRIAQRYSGWNS
                     ELGQQILKGQMSLADLAKYAQEHNLSPVHQSGRQEQLENLVNHYLFDK"
     gene            3239..4231
                     /gene="xylF"
                     /locus_tag="NBI74_003427"
     CDS             3239..4231
                     /gene="xylF"
                     /locus_tag="NBI74_003427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter substrate-binding
                     protein"
                     /protein_id="HCD1933709.1"
                     /translation="MKIKNILLTLCTSLLLTNVAAHAKEVKIGMAIDDLRLERWQKDR
                     DIFVKKAESLGAKVFVQSANGNEETQMSQIENMINRGVDVLVIIPYNGQVLSNVVKEA
                     KQEGIKVLAYDRMINDADIDFYISFDNEKVGELQAKALVDIVPQGNYFLMGGSPVDNN
                     AKLFRAGQMKVLKPYVDSGKIKVVGDQWVDGWLPENALKIMENALTANNNKIDAVVAS
                     NDATAGGAIQALSAQGLSGKVAISGQDADLAGIKRIAAGTQTMTVYKPITLLANTAAE
                     IAVELGNGQEPKADTTLNNGLKDVPSRLLTPIDVNKNNIKDTVVKDGFHKESEL"
     gene            4309..5850
                     /gene="xylG"
                     /locus_tag="NBI74_003428"
     CDS             4309..5850
                     /gene="xylG"
                     /locus_tag="NBI74_003428"
                     /EC_number="7.5.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter ATP-binding protein"
                     /protein_id="HCD1933710.1"
                     /translation="MPYLLEMKNITKTFGSVKAIDNVSLRLNAGEIVSLCGENGSGKS
                     TLMKVLCGIYPHGSYEGEIIFAGEEIQASHIRDTERKGIAIIHQELALVKELTVLENI
                     FLGNEITHNGIMDYDLMTLRCQKLLAQVSLSISPDTRVGDLGLGQQQLVEIAKALNKQ
                     VRLLILDEPTASLTEQETSVLLDIIRDLQQHGIACIYISHKLNEVKAISDTICVIRDG
                     QHIGTRDAAGMSEDDIITMMVGRELTALYPNEPHTTGDEILRIEHLTAWHPVNRHIKR
                     VNDVSFSLKRGEILGIAGLVGAGRTETIQCLFGVWPGQWEGKIYIDGKQVDIRNCQQA
                     IAQGIAMVPEDRKRDGIVPVMAVGKNITLAALKKFTGSISQLDDAAEQKCILESIQQL
                     KVKTSSPDLAIGRLSGGNQQKAILARCLLLNPRILILDEPTRGIDIGAKYEIYKLINQ
                     LVQQGIAVIVISSELPEVLGLSDRVLVMHEGKLKANLINHNLTQEQVMEAALRSEHHV
                     EKQSV"
     gene            5828..7009
                     /gene="xylH"
                     /locus_tag="NBI74_003429"
     CDS             5828..7009
                     /gene="xylH"
                     /locus_tag="NBI74_003429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylose ABC transporter permease XylH"
                     /protein_id="HCD1933711.1"
                     /translation="MSKSNPSEVKLAVPTSGGFSGLKSLNLQVFVMIAAIIAIMLFFT
                     WTTDGAYLSARNVSNLLRQTAITGILAVGMVFVIISAEIDLSVGSMMGLLGGVAAICD
                     VWLGWPLPLTIIVTLVLGLLLGAWNGWWVAYRKVPSFIVTLAGMLAFRGILIGITNGT
                     TVSPTSAAMSQIGQSYLPASTGFIIGALGLMAFVGWQWRGRMRRQALGLQSPASTAVV
                     GRQALTAIIVLGAIWLLNDYRGVPTPVLLLTLLLLGGMFMATRTAFGRRIYAIGGNLE
                     AARLSGINVERTKLAVFAINGLMVAIAGLILSSRLGAGSPSAGNIAELDAIAACVIGG
                     TSLAGGVGSVAGAVMGAFIMASLDNGMSMMDVPTFWQYIVKGAILLLAVWMDSATKRR
                     S"
     gene            7087..8265
                     /gene="xylR"
                     /locus_tag="NBI74_003430"
     CDS             7087..8265
                     /gene="xylR"
                     /locus_tag="NBI74_003430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator XylR"
                     /protein_id="HCD1933712.1"
                     /translation="MFTKRHRITLLFNANKAYDRQVVEGVGEYLQASQSEWDIFIEED
                     FRARIDKIKDWLGDGVIADFDDKQIEQALADVDVPIVGVGGSYHLAESYPPVHYIATD
                     NYALVESAFLHLKEKGVNRFAFYGLPESSGKRWATECEYAFRQLVAEEKYRGVVYQGL
                     ETAPENWQHAQNRLADWLQTLPPQTGIIAVTDARARHILQVCEHLHIPVPEKLCVIGI
                     DNEELTRYLSRVALSSVAQGARQMGYQAAKLLHRLLDKEEMPLQRILVPPVRVIERRS
                     TDYRSLTDPAVIQAMHYIRNHACKGIKVDQVLDAVGISRSNLEKRFKEEVGETIHAMI
                     HAEKLEKARSLLISTTLSINEISQMCGYPSLQYFYSVFKKAYDTTPKEYRDVNSEVML
                     "
     gene            complement(8373..9197)
                     /locus_tag="NBI74_003431"
     CDS             complement(8373..9197)
                     /locus_tag="NBI74_003431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein bax"
                     /protein_id="HCD1933713.1"
                     /translation="MILTPIRRYGAMILMLLTLVFSSEVLAKTHTTTASQKSHLTKAS
                     NKQVSSKQEYSRNSAKSSSLPDLRKYPSGTPRKKAFLRTVMPYITSQNAAITAERNWL
                     ISKQYQGQWSPAERARLKDIAKRYKVKWSGNTRKIPWNTLLERVDIIPTSMVATMAAA
                     ESGWGTSKLARNNNNLFGMKCMKGRCTNAPGKVKGYSQFSSVKESVSAYVTNLNTHPA
                     YSSFRKSRAQLRKADQEVTATAMIHKLKGYSTKGKSYNNYLFAMYQDNQRLIAAHM"
     gene            9517..11547
                     /gene="malS"
                     /locus_tag="NBI74_003432"
     CDS             9517..11547
                     /gene="malS"
                     /locus_tag="NBI74_003432"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HCD1933714.1"
                     /translation="MKLAACFLTLLPGFAVAASWTSPGFPAFSEQGTGTFVSHAQLPK
                     GTRPLTLNFDQQCWQPADAIKLNQMLSLQPCSNTPPQWRLFRDGEYTLQLDTRSGTPT
                     LMISIQNAVEPVASLVRECPKWDGLPLTLDVSATFAEGAAVRDYYSQQIAIVKNGQIT
                     LQPAATSNGLLLLERAETDTSAPFDWHNATVYFVLTDRFENGDPSNDQSYGRHKDGMA
                     EIGTFHGGDLRGLTNKLDYLQQLGVNALWISAPFEQIHGWVGGGTKGDFPHYAYHGYY
                     TQDWTNLDANMGSEADLRTLVDSAHQRGIRILFDVVMNHTGYATLADMQEYQFGALYL
                     SGDEVKKTLGERWNDWKPAAGQTWHSFNDYINFSDKTGWDKWWGKNWIRTDIGDYDNP
                     GFDDLTMSLAFLPDIKTESTTASGLPVFYKNKTDTHAKIIDGFTPRDYLTHWLSQWVR
                     DYGIDGFRVDTAKHVELPAWQQLKTEASAALHEWKKANPDKALDDKPFWMTGEAWGHG
                     VMQSDYYRHGFDAMINFDYQEQAAKAVDCLAQMDTTWQQMAEKLQDFNVLSYLSSHDT
                     RLFREGGDKAAELLLLAPGAVQIFYGDESSRPFGPTGSDPLQGTRSDMNWQDVSGKSA
                     ASVAHWQKISQFRARHPAIGAGKQTTLSLKQGYGFVREHGDDKVLVIWAGQQ"
     gene            11725..12978
                     /gene="avtA"
                     /locus_tag="NBI74_003433"
     CDS             11725..12978
                     /gene="avtA"
                     /locus_tag="NBI74_003433"
                     /EC_number="2.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--pyruvate transaminase"
                     /protein_id="HCD1933715.1"
                     /translation="MTFSLFGDKFTRHSGITLLMEDLNDGLRTPGAIMLGGGNPAQIP
                     EMQDYFQTLLTDMLESGKATDALCNYDGPQGKTELLTLLAGMLREKLGWDIEPQNIAL
                     TNGSQSAFFYLFNLFAGRRADGRVKKVLFPLAPEYIGYADAGLEEDLFVSARPNIELL
                     PEGQFKYHVDFEHLHIGEETGMICVSRPTNPTGNVITDEELLKLDALANQHGIPLVID
                     NAYGVPFPGIIFSEARPLWNPNIVLCMSLSKLGLPGSRCGIIIANEKIITAITNMNGI
                     ISLAPGGIGPAMMCEMIKRNDLLRLSETVIKPFYYQRVQETIAIIRRYLPEDRCLIHK
                     PEGAIFLWLWFKDLPITTEQLYQRLKARGVLMVPGHNFFPGLDKPWPHTHQCMRMNYV
                     PEPEKIEAGVKILAEEIERAWAESH"
     gene            complement(13017..13490)
                     /locus_tag="NBI74_003434"
     CDS             complement(13017..13490)
                     /locus_tag="NBI74_003434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HCD1933716.1"
                     /translation="MNRFIIADATKCIGCRTCEVACAVSHQENQDCAALSPDEFISRI
                     RVIKDHSWTTAVACHQCEDAPCANVCPVDAISREHGHIFVEQSRCIGCKSCMLACPFG
                     AMEVVSSRKKARAIKCDLCWHRATGPACVEACPTKALQCMDVEKVQRHRLRQQPV"
     gene            complement(13592..14440)
                     /gene="yiaJ"
                     /locus_tag="NBI74_003435"
     CDS             complement(13592..14440)
                     /gene="yiaJ"
                     /locus_tag="NBI74_003435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator YiaJ"
                     /protein_id="HCD1933717.1"
                     /translation="MGKEVMGKKENEMAQEKERPAGSQSLFRGLMLIEILSNYPNGCP
                     LAHLSELAGLNKSTVHRLLQGLQSCGYVTTAPAAGSYRLTTKFIAVGQKALSSLNIIH
                     IAAPHLEALNIATGETINFSSREDDHAILIYKLEPTTGMLRTRAYIGQHMPLYCSAMG
                     KIYMAFGHPDYVKSYWESHQHEIQPLTRNTITELPAMFDELAHIRESGAAMDREENEL
                     GVSCIAVPVFDIHGRVPYAVSISLSTSRLKQVGEKNLLKPLRETAQAISNELGFTVRD
                     DQGAIT"
BASE COUNT         3681 a         3691 c         3567 g         3580 t
ORIGIN      
        1 cacccggtca ggcaggggat aacgtttacg ccattaatgg cagaagttgc tgatagaggc
       61 gacggaacgt ttctcgtcgt ggctgataag cggcataacg ctgcgcatct ggtagatgcg
      121 actgttctaa cggtagttgc ggcaacaact caatgagcga tttctctgga ttcgccgcaa
      181 tctgcgccag ccttgctgcg cccagtgctg gccccacatc gccacccgtg cggtaatcca
      241 gttgctgacc gctaatgtcc gccagcattt gacgccagta ctcactacgc gccccgccac
      301 caatcaacgt aacactttgc ggtttaatgc cgcaggcatg cacgacatcc atgccatctg
      361 ccagcgcata acccacgcct tccagcactg ctcgcgccag ttcattgggg ccatgttgat
      421 gagtcaaacc aaagaaaacc cctttcgcct ggggattatt gtgtggtgta cgctcgccag
      481 aaagataagg cagaaaccaa actggctcgg cactttcatc agcctgtcga gctgcagtga
      541 ttaaagcagg gacattgctc aggccggtta atttcgcggc ccaatccaga cacgaagccg
      601 cactcagcat tacagacatt aaatgccaac gttgcggtaa cgcatggcaa aagctatgta
      661 cggcgctttc tggcttgctt aagaaccctt cgctgacagc aaaatagacc cccgacgtcc
      721 ccagcgataa cattgcctga ttagcatcaa ccattcccac accaaccgca ccagctgcat
      781 tgtcgccacc gcctgcgaca actggcaccg ccgccatacc ccacgctttc gcaacttcag
      841 gtagcaaagc accagtaatt tcgctgcctt cgtataatgc gggcatctgg tcacgagata
      901 agtggcaagc ctgcagcatg tcgtcactcc agtcacgctt tgcgacatcc agccacatgg
      961 tgccagctgc gtcagacata tcgctggcaa actcccccgt catacgcaga cgcaagtaat
     1021 ctttcggtaa taatacttta tcgatttgac ggaatatctc cggctcatgc cgctgaaccc
     1081 atagcaattt aggcgcagta aatccgggca tcatcaggtt gccggtaatc acccgcgatt
     1141 gcggaactcg cgcttccagc aaagtgcact cttgcgcaca gcgcccgtcg ttccacaaaa
     1201 tggcagggcg taacacccgt tgctgagcat ccagtaaggt tgcaccatgc atctgtccgg
     1261 caatacctaa tgctttaacg tcttgcagag aatgctgaac gcccagagct ttcattgcgc
     1321 gatcagttgc ctgccaccac tgttccgggt cttgttccga ccagagtgga tgcgggcgcg
     1381 aaacggtcag cttttccgtt tgcgcagcaa ccacctcacc ctgctcgttg agcaaaataa
     1441 cttttacgcc cgaggtgcca agatctatcc cgatatacat atcgatcgtt ccttaaaaaa
     1501 tgcccggtat cgctaccgat aaccgggcca acggactgca cagttaaccg ttatttgtcg
     1561 aacagataat gatttaccag attttccagt tgctcctggc gaccactctg atgcaccgga
     1621 gacaaattat gttcctgagc atatttggct aaatctgcca gtgacatttg gcctttcagg
     1681 atttgctggc ctaattcact attccagccg gaataacgct gcgcgatgcg tttatccagc
     1741 tcgccatctt caatcatgcg cgctgcaatt ttcagcgcca acgccatcgt atccatcgcg
     1801 ccgatatgac cgtaaaacag atcatattta tcagtacttt gacgacgtac tttggcatcg
     1861 aagttcagac caccggtggt gaaaccgcct gctttgagaa tttcatacat caccagcgca
     1921 ttctcttcca cactgttcgg gaactggtcg gtgtcccagc ccagttgcgc atcgccacgg
     1981 ttggcgtcga cagaaccgaa caggccaagc gcaatggcgg tggctatttc atgatggaaa
     2041 gagtgacctg ccagcgtcgc gtggttagct tcaatgttca gtttaatctc tttttccaga
     2101 ccaaactgtt tcaggaagcc atagaccgtc gcagcatcgt aatcatattg atgtttggtc
     2161 ggttcttgcg gtttcggttc gataagcaac gtgccctgga aaccgatttt atgtttatgc
     2221 tcaaccacca tctgcataaa gcggcccagc tgttcacgct cctggcgcaa gtcggtattt
     2281 aacagcgttt cgtaaccttc acgaccaccc cacaggacat agttttcacc gcccaattta
     2341 tgggttgctt ccatcgctgt aacaacttgc gttgccgccc aactgaagac ttcaggatct
     2401 gggttcgtcg ccgcacccgc gccatagcga gggtttgtaa agcagttagc agttccccac
     2461 agcagcttca cgccgctctc ttcttgcttg cctgccagga catcaaccat ttgcgcaaaa
     2521 ttattgatgt actcttttaa cgacgcgccc tcaggggaaa catccacatc gtggaagcaa
     2581 taaaatggca catgtaactt gtggaaaaac tcaaatgcga catctgcttt acgcttcgcc
     2641 aacgccagtg cctcaccagg ctgctgccac ggacgattaa acgcccccac accaaacata
     2701 tccgccccgt tccagcagaa ggtgtgccag tagcaggcgg caaaacgcaa gtgctcttcc
     2761 atacgcttgc ccaacaccag ttcgtcggga ttgtagtgac ggaatgctaa cgggtttgag
     2821 gattttgagc cttcataacg aacgcgatcg agctggtcaa aataggcttg catattgaac
     2881 tccataatca ggtaatgccg cgggtgatgg atgatgtcgt aatattgggc actccctttc
     2941 agttgctcaa ttatgttatt tcacactgct attgagataa ttcacaagtg tgcgctcgct
     3001 cgcaaaataa aatgggatga tgaaactggg taattcctcg aagagaaaaa tgcaataagt
     3061 acaattgcgc aacaaaagta agatctcggt cataaatcaa gaaataaacc aaaaatcgta
     3121 atcgaaagat aaaaatctgt aattgttttc ccctgtttag ttgctaaaaa ttggttacgt
     3181 ttatcgcggt gattgttact tattaaagct gtcctctaac tacagaaggc cctacaccat
     3241 gaaaataaag aacattctac tcaccctttg cacctcactt ctgcttacca acgttgcggc
     3301 acacgccaaa gaagtcaaaa taggtatggc gattgatgat ctccgtcttg aacgctggca
     3361 aaaagatcga gatatttttg tgaaaaaagc agaatctctc ggcgcgaaag tatttgtaca
     3421 gtctgcaaat ggcaatgaag aaacacaaat gtcgcagatt gaaaacatga ttaaccgggg
     3481 tgtcgatgtt cttgtcatta ttccttataa cggtcaggta ttaagtaacg ttgtaaaaga
     3541 agccaaacaa gaaggtatta aagtattagc ttacgaccgt atgattaacg atgcggatat
     3601 cgatttttat atttctttcg ataacgaaaa agtcggtgaa ctgcaggcaa aagccctggt
     3661 cgatattgtt ccgcaaggta attacttcct gatgggcggc tcgccggtag ataacaacgc
     3721 caagctgttc cgcgccgggc aaatgaaagt attaaaacct tatgttgatt ccggaaaaat
     3781 taaagtcgtt ggtgaccaat gggttgatgg ctggttaccg gaaaacgcat tgaaaattat
     3841 ggaaaacgcg ctaaccgcca ataataacaa aattgatgct gtagttgcct caaacgatgc
     3901 caccgcagga ggggcaattc aggcattaag cgcgcaaggt ttatcaggga aagtagcaat
     3961 ttccggccag gatgcggatc ttgcaggtat taaacgtatt gctgccggta cgcaaactat
     4021 gacggtgtat aaacctatta cattgttggc aaatactgcc gcagaaattg ccgttgagtt
     4081 gggcaatggt caggagccaa aagcggatac cacactgaat aatggcctga aagatgttcc
     4141 ctcccgcctg ctgacaccga tcgatgtgaa taaaaacaac atcaaagata cggtagttaa
     4201 agacggattc cacaaagaga gcgagctgta agcgttacgc cccggcgcgg agcgggggcg
     4261 tgatttctct ccatgccgcg ttaattaatt ggcttaggtg gagtcgttat gccttatcta
     4321 cttgaaatga aaaatattac caaaaccttc ggcagtgtga aggcgattga taacgtcagc
     4381 ttgcggttga atgctggcga aatcgtctca ctctgtgggg aaaatgggtc aggtaaatca
     4441 acgctgatga aagtgctgtg tggtatttat ccccatggtt cctacgaagg cgaaattatt
     4501 tttgcgggag aagagattca ggcgagtcac atccgcgata ccgaacgcaa aggtatcgcc
     4561 attattcacc aggaattggc gctggtgaaa gaactgaccg tgctggaaaa tatcttcctg
     4621 ggtaacgaaa taacccataa tggcattatg gattatgacc tgatgacgct acgctgtcag
     4681 aagctgctcg cacaggtcag tttatccatt tcacctgata cccgcgttgg cgatttagga
     4741 cttgggcaac aacaactggt tgaaattgcc aaggcactta ataaacaggt gcgtttgtta
     4801 attctcgatg agccgacagc ctcattaact gagcaggaaa cgtcggtttt actggatatt
     4861 attcgcgatc tacaacagca cggtatcgcc tgtatttata tttcgcacaa actcaacgaa
     4921 gtcaaagcga tttccgatac gatttgcgtt attcgcgacg gtcaacacat tggtacgcgt
     4981 gatgctgctg gaatgagtga agacgatatt atcaccatga tggtcgggcg agagttaacc
     5041 gcgctttacc ctaatgaacc acataccacc ggagatgaaa tattacgtat tgaacatctg
     5101 acggcatggc atccggttaa tcgtcatatt aaacgagtta atgatgtctc gttttccctg
     5161 aaacgtggcg aaatactggg tatcgccgga ctcgttggtg ccggacgtac cgagactatt
     5221 cagtgcctgt ttggtgtgtg gcccggacaa tgggaaggga aaatttatat tgatggcaaa
     5281 caggtagata ttcgtaactg tcagcaagcc atcgcccagg ggattgcgat ggtacccgaa
     5341 gacagaaagc gcgacggcat cgttccggta atggcggttg gtaaaaatat taccctcgcc
     5401 gcactcaaaa aatttaccgg tagtattagc cagcttgatg acgcggcaga gcaaaaatgt
     5461 attctggaat caatccagca actcaaagtt aaaacgtcgt cccccgacct tgctattgga
     5521 cgtttgagcg gcggcaatca acaaaaagcg atcctcgctc gctgtctgtt acttaacccg
     5581 cgcattctca ttcttgatga acccaccagg ggtatcgata ttggcgcgaa atacgagatc
     5641 tacaaattaa ttaaccaact cgtccagcag ggtattgccg ttattgtcat ctcttccgaa
     5701 ttacctgaag tgctcggcct tagcgatcgt gtactggtga tgcatgaagg gaaactaaaa
     5761 gccaacctga taaatcataa cctgactcag gagcaggtga tggaagccgc attgaggagc
     5821 gaacatcatg tcgaaaagca atccgtctga agtgaaatta gccgtaccga catccggtgg
     5881 cttttccggg ctgaaatcac tgaatttgca ggtcttcgtg atgattgcag ctatcatcgc
     5941 aatcatgctg ttctttacct ggaccaccga tggtgcctac ttaagcgccc gtaacgtctc
     6001 caacctgtta cgccaaaccg caattaccgg catcctcgcg gtaggaatgg tgttcgtcat
     6061 aatttctgct gaaatcgacc tttccgtcgg ctcaatgatg gggctgttag gtggcgtcgc
     6121 ggcgatttgt gacgtctggc taggctggcc tttgccactt accatcattg tgacgttggt
     6181 tctgggactg cttctcggag cctggaacgg atggtgggtc gcgtaccgca aagtcccttc
     6241 atttattgtc accctcgcgg gcatgttggc atttcgtggc atactcattg gcatcaccaa
     6301 cggcacgact gtttccccca ccagcgccgc gatgtcacaa attgggcaaa gctatctacc
     6361 tgccagcacc ggcttcatca ttggcgcgct tggcttaatg gcttttgttg gctggcaatg
     6421 gcgcggaaga atgcgccgtc aggctttggg tttgcagtct ccggcctcta ccgcagtagt
     6481 cggtcgccag gctttaaccg ctatcatcgt attaggcgca atctggctgt tgaatgatta
     6541 ccgtggtgtt cccactcctg ttctgctgct aacgttactg ttattgggcg gcatgtttat
     6601 ggcaacgcgg acggcatttg gacggcgcat ttatgccatc ggcggcaatc tggaagcggc
     6661 acgtctctcc gggattaacg ttgaacgcac caaacttgcc gtgttcgcta ttaacggatt
     6721 aatggtagcc atcgccggat taatcctcag ttctcgactc ggcgctggtt caccttctgc
     6781 gggaaatatc gccgaactgg acgcaattgc agcatgtgtg attggcggca ccagtctggc
     6841 aggcggcgtc gggagcgtgg ccggagcggt aatgggggca ttcatcatgg cttcgctgga
     6901 taacggcatg agtatgatgg atgtaccgac cttctggcag tatatcgtta aaggtgcgat
     6961 tctgttgctg gcagtatgga tggactccgc aaccaaacgc cgttcttgat tttgataaaa
     7021 attttctcaa agccggtaac gtattaccgg ttttgagttt ttgcatgatt cagcaggaaa
     7081 agaaccatgt ttactaaacg tcaccgcatc acattactgt tcaatgccaa taaagcctat
     7141 gaccggcagg tagtagaagg cgtaggggaa tatttacagg cgtcacaatc ggaatgggat
     7201 attttcattg aagaagattt ccgcgcccgc attgataaaa tcaaggactg gttaggagat
     7261 ggcgtcattg ccgacttcga cgacaaacaa atcgagcaag cgctggctga tgtcgacgtc
     7321 cccattgttg gggttggtgg ctcgtatcac cttgccgaaa gttacccacc cgttcattac
     7381 attgccaccg ataactatgc gctggttgaa agcgcatttt tgcatttaaa agagaaaggc
     7441 gttaaccgct ttgcttttta tggtcttccg gaatcaagcg gtaaacgttg ggccactgaa
     7501 tgcgaatatg catttcgtca gcttgtcgct gaagaaaagt atcgcggagt ggtttatcag
     7561 gggttagaaa ccgcgccaga gaactggcaa cacgcgcaaa atcggctggc agactggcta
     7621 caaacgctac caccgcaaac cgggattatt gccgttactg acgcccgggc gcggcatatt
     7681 ctgcaagtat gtgaacatct acatattccc gtaccggaaa aattatgcgt gattggcatc
     7741 gataacgaag aactgacccg ctatctgtcg cgtgtcgccc tttcttcggt cgctcagggc
     7801 gcgcggcaaa tgggctatca ggcggcaaaa ctgttgcatc gattattaga taaagaagaa
     7861 atgccgctac agcggatttt ggtcccacca gttcgcgtca ttgaacggcg ctcaacagat
     7921 taccgctcgc tgaccgatcc cgccgttatt caggctatgc attacattcg taatcacgcc
     7981 tgtaaaggga ttaaagtgga tcaggtactg gatgcggtgg gcatatcacg ctccaatctt
     8041 gagaagcgtt ttaaagaaga ggtgggtgaa accatccatg ccatgattca tgccgaaaag
     8101 ctggagaaag cgcgcagtct gctgatttca accaccttgt cgatcaatga gatatcgcaa
     8161 atgtgcggtt atccatcgct gcaatatttc tactctgttt ttaaaaaagc atatgacacg
     8221 acgccaaaag agtatcgcga tgtaaatagc gaggtcatgt tgtagcgatg atgagaattg
     8281 tcggcgtcgc ttcaggtaac tcagcacaac cacctgatgg gagcaaaagc cttatccgac
     8341 tgacaagtcg gataaggctc gaggaaatgc aattacatat gcgccgcgat taaccgttgg
     8401 ttatcctggt acattgcaaa caggtagttg ttataactct tccctttggt cgagtagccc
     8461 ttcagcttgt gaatcatcgc tgtggcagtc acttcctgat ccgctttacg cagctgtgca
     8521 cgcgatttac ggaacgaaga gtaagccggg tgcgtattga ggtttgtaac ataggcgctc
     8581 accgattctt taacagaact aaactgtgag taccctttca ctttacccgg cgcattggta
     8641 caacgtcctt tcatgcattt catgccgaaa agattgttgt tgttgcgcgc cagctttgac
     8701 gttccccaac cgctttctgc tgcagccatc gtcgccacca tactggtagg gataatgtct
     8761 acgcgttcaa gcaaggtatt ccacgggatt tttcgcgtat taccagacca cttcaccttg
     8821 tagcgtttgg cgatgtcttt cagacgcgca cgctcagcag gtgaccattg gccctgatac
     8881 tgttttgaaa tcagccagtt acgttccgca gtaatggccg cattttggct ggtaatgtaa
     8941 ggcattacag tccggagaaa cgcctttttc cttggtgttc cggaagggta ttttcgcaaa
     9001 tcaggaagtg aactactctt tgcactattg cgagaatact cttgtttact gcttacctgt
     9061 ttattactag ctttagttaa gtgggacttt tgactcgctg ttgttgtgtg cgtcttcgct
     9121 aacacctcac tcgaaaacac cagagtgagt aacataagaa tcatcgcccc atatcgtcgt
     9181 atgggagtca aaatcatcag gtctcctggt cggatttaat cattccaaca ccttatattt
     9241 tttacaaatt tgagagttga atctcaaatc atatcaaaaa tagctgtcaa gagcacccca
     9301 aggaatagtc caaatctgaa actatgtcac gagttaacga ttcagattgg cgctaaatcg
     9361 cagaaaatgt ggggtttatc gcaaaattca gccgtttttt gcgcgcgatc gctcaccctt
     9421 gcttctcatc ctgtggagtt accgctcagg gatgagtttt gtctggctta tcgctggcaa
     9481 actgtctgaa atcgcagcaa taaggactca tccgccatga aactcgccgc ctgttttctg
     9541 acactccttc ctggcttcgc cgttgccgcc agctggactt ctccggggtt tcccgccttt
     9601 agcgaacagg ggacgggaac atttgtcagc cacgcacagt tgcccaaagg tacgcgtcca
     9661 ctaacgctaa attttgacca acagtgctgg cagcctgcgg atgcgataaa actcaatcag
     9721 atgctttccc tgcaaccttg tagcaacacg ccgcctcaat ggcgattgtt cagggacggc
     9781 gaatatacgc tgcaactaga cacccgatcc ggtacaccaa cattgatgat ttccatccag
     9841 aacgccgtcg aaccggtagc aagcctggtc cgtgaatgcc cgaaatggga tggattaccg
     9901 ctcacactgg atgtcagcgc cacttttgcg gagggagccg cagtccggga ttattacagc
     9961 cagcaaattg cgatagtgaa gaacggtcaa ataacgttac aacccgctgc caccagcaac
    10021 ggtttactcc tgctggaacg ggcagaaact gacacatccg cccctttcga ctggcataac
    10081 gccacggttt actttgtgct gacagatcgt ttcgaaaacg gcgatcccag taatgaccag
    10141 agttacggac gtcataaaga cggtatggcg gaaattggca cttttcacgg cggcgattta
    10201 cgcggcctga ccaacaaact ggattacctc cagcaactgg gcgtcaatgc tttatggata
    10261 agtgccccat ttgagcaaat tcacggctgg gtcggcggcg gtacaaaagg tgatttcccg
    10321 cattatgcct atcatggtta ttacacacag gactggacga atcttgatgc caatatgggc
    10381 agtgaagccg atctacggac gctggttgat agcgcgcatc agcgcggtat tcgtattctc
    10441 tttgatgtcg tgatgaacca cactggctat gccacgctgg cggatatgca ggagtatcag
    10501 tttggcgcgt tatatctttc tggtgacgaa gtgaaaaaaa cgctgggtga acgctggaac
    10561 gactggaaac ctgccgccgg gcaaacctgg catagcttta acgattacat taatttcagc
    10621 gacaaaacag gctgggataa atggtgggga aaaaactgga tcagaacgga tatcggcgat
    10681 tacgacaatc ctggattcga cgatctcact atgtcgctgg cctttttgcc ggatatcaaa
    10741 accgaatcaa ctaccgcttc tggtctgcca gtgttctata aaaacaaaac ggatacccac
    10801 gctaaaatca tcgacggctt tacccctcgc gattacttaa cccactggtt aagtcagtgg
    10861 gtccgcgact atgggattga tggttttcgg gtcgataccg ccaaacatgt tgagttgccc
    10921 gcctggcagc aactgaaaac cgaagccagc gctgcacttc acgaatggaa aaaagctaac
    10981 cccgacaaag cattagatga caaacctttc tggatgaccg gtgaagcctg gggccacggc
    11041 gtgatgcaaa gtgactacta tcgccacggc ttcgatgcga tgatcaattt cgattatcag
    11101 gagcaggcgg cgaaagctgt cgattgtctg gcgcagatgg atacgacctg gcagcaaatg
    11161 gcggagaaat tgcaggattt caacgtgttg agctacctct cgtcgcatga tacccgcctg
    11221 ttccgtgaag ggggcgacaa agcagcagag ttattattat tagcaccagg cgcggtacaa
    11281 attttttatg gcgatgaatc ctcgcgtccg ttcggtccta ccggttctga tccgctgcaa
    11341 ggtacgcgtt cggatatgaa ctggcaggat gttagcggta aatctgccgc cagcgtcgcg
    11401 cactggcaga aaatcagcca gttccgcgcc cgccatcccg caattggcgc gggcaaacaa
    11461 acgacacttt cgctgaagca gggctacggc tttgttcgtg agcatggcga cgataaagtg
    11521 ctggtcatct gggcggggca acagtgactt ttccggcttc ccgttcgtga gtacctcggg
    11581 aagccgccaa ccaggataaa atgtcagccc taatcagcat tgcaggataa agccccgctc
    11641 actcttcaac agaccgattt gcaccccagc aaatgtagcg ttattgttac cttccttgct
    11701 acagagttcg acagatatcc cgctatgaca ttctcccttt ttggtgacaa atttacccgc
    11761 cactccggca ttacgctgct gatggaagat ctgaacgacg gtttacgcac gcctggcgcg
    11821 attatgctcg gcggcggtaa tccggcgcag atcccggaaa tgcaggacta cttccagacg
    11881 ctactgaccg acatgctgga aagtggcaaa gcgactgatg cactgtgcaa ttacgacggt
    11941 ccacagggga aaacggagct actcacactg cttgccggaa tgctgcgcga gaagttgggc
    12001 tgggatatcg aaccacagaa tattgcacta acaaacggca gccagagcgc gtttttctac
    12061 ttatttaatc tgtttgccgg acgccgtgcc gatggtcggg tcaaaaaagt gctgttcccg
    12121 ctggcaccgg aatacattgg ctacgctgac gccggactgg aagaagatct gtttgtctct
    12181 gcgcgtccga atattgaact gctgccggaa ggccagttta aataccacgt cgattttgag
    12241 catctacata ttggcgaaga aaccgggatg atttgcgtct cccggccgac gaatccaaca
    12301 ggcaatgtga ttactgacga agagttgctg aagcttgacg cgctggcgaa tcaacacggc
    12361 attccgctgg tgattgataa cgcttatggc gtcccgttcc cgggtatcat cttcagcgaa
    12421 gcgcgcccgc tatggaatcc gaatatcgtg ctgtgcatga gtctttccaa gctggggcta
    12481 cctggctccc gctgcggcat tatcatcgcc aatgaaaaaa tcatcaccgc catcaccaat
    12541 atgaacggca ttatcagcct ggcacctggc ggtattggtc cggcgatgat gtgtgaaatg
    12601 attaagcgta acgatctgct gcgcctgtct gaaacagtca tcaaaccgtt ttactaccag
    12661 cgtgttcagg aaactatcgc catcattcgc cgctatttac cggaagatcg ctgcctgatt
    12721 cataaaccgg aaggagccat tttcctctgg ctatggttta aggatttgcc cattacgacc
    12781 gagcagctct accagcgcct gaaagcacgc ggcgtgctga tggtgccggg gcacaacttc
    12841 ttcccgggac tggataaacc gtggccgcat acgcatcaat gtatgcgcat gaactacgta
    12901 ccagagccgg agaaaattga ggcgggtgtg aagattctgg cggaagagat agaaagagcc
    12961 tgggctgaaa gccattaata cgctattccg gcgttgagaa acgccggaaa aacgtttcaa
    13021 acaggctgct gccgtagccg atgccgctgc actttctcga catccatgca ctgcaacgcc
    13081 tttgtcgggc aggcttcaac acaggccggc cccgtcgccc gatgccagca cagatcgcac
    13141 ttaatcgccc tcgccttttt gcgcgaagaa acgacctcca tcgcaccaaa cgggcaagcc
    13201 agcatacagc ttttacagcc aatgcaacgt gattgttcaa cgaaaatatg cccatgttcg
    13261 cggcttatcg cgtcaacagg gcagacattc gcgcacggtg catcttcaca ctgatgacag
    13321 gctactgccg tggtccagct gtggtcttta atgacacgaa tacgggaaat aaactcgtct
    13381 ggtgacaacg cagcgcaatc ctgattctcc tgatgcgaca ctgcgcaagc cacttcacag
    13441 gtacggcaac cgatacattt cgtcgcatcc gcaataataa accggttcat gatcactcct
    13501 ttgcactaaa acagcagagt gccagagagc gcgtccctac tgctttgatc caacgaagga
    13561 aatgttaaag ttttggcact tgtccaaagg gttatgtaat cgcgccctgg tcgtccctga
    13621 cagtaaatcc cagctcatta gaaatagcct gcgcggtttc acgcagtggt ttcaggagat
    13681 ttttctctcc cacctgtttc agacgtgatg tcgaaagcga aatcgacacg gcgtacggca
    13741 cccgcccatg aatatcaaac accggaacag caatacagga gacgccgagt tcgttttctt
    13801 ctctgtccat cgccgctcca ctttcacgaa tgtgcgccag ttcgtcgaac atcgcgggca
    13861 gctcggtaat ggtattgcgg gttaacggct ggatctcatg ctgatggctt tcccagtatg
    13921 acttcacgta gtccgggtga ccaaacgcca tgtagatctt gcccattgcg gaacagtaga
    13981 gcggcatatg ctggcctata taggcacggg ttcgcagcat cccggttgtg ggttccagct
    14041 tataaatcaa aatagcgtga tcgtcttcgc ggctggagaa gttaatggtt tcaccagtgg
    14101 cgatgttcag tgcctcaaga tgcggagcgg cgatatgaat gatattcagc gaagatagcg
    14161 ccttctgccc gacggcaata aatttggtgg tcaggcgata actccctgcg gcgggcgcgg
    14221 tggtcacata gccgcaggac tgtaatccct gcaataagcg atggacggta ctcttattta
    14281 aaccagccag ctccgaaaga tgcgccaacg gacaaccgtt tggatagttg ctcaaaatct
    14341 caatcagcat caacccacga aacagactct ggcttccggc tggacgctct ttttcctgcg
    14401 ccatctcgtt ctcttttttt cccatcactt ctttccccat tttgtcgcgt cctgatggta
    14461 gcgcaaagtg tgccgtagtt cacgatctcg acagataatt tataaccact tgattttta
//
LOCUS       DAHHTG010000110        13891 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.111, whole genome shotgun sequence.
ACCESSION   DAHHTG010000110 DAHHTG010000000
VERSION     DAHHTG010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13891)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13891)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13891
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.111"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            <1..210
                     /locus_tag="NBI74_003436"
     CDS             <1..210
                     /locus_tag="NBI74_003436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020237521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pas-pac-pac sensing his kinase"
                     /protein_id="HCD1933718.1"
                     /translation="GIKLKNETTYVSLPNPTILITTTSTPERQFSISVLDDDNKENLP
                     TDTAPCFIIGIPDEYTKAIELLNIK"
     gene            388..1311
                     /locus_tag="NBI74_003437"
     CDS             388..1311
                     /locus_tag="NBI74_003437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HCD1933719.1"
                     /translation="MGSTRKGMLNVLIAAVLWGSSGVCAQYIMEQSQMSSQFLTMTRL
                     IFAGLILLTLSFVHGDKIFSIINNHKDAISLLIFSVVGALTVQLTFLLTIEKSNAATA
                     TVLQFLSPTIIVAWFSLVRKSRPGILVFCAILTSLIGTFLLVTHGNPTSLSISPAALF
                     WGIASAFAAAFYTTYPSTLIARYGTLPVVGWSMLIGGLILLPFYARQGTNFVVNGSLI
                     LAFFYLVVIGTSLTFSLYLKGAQLIGGPKASILSCAEPLSSALLSLLLLGITFTLPDW
                     LGTLLILSSVILISMDSRRRARKINRPARHE"
     gene            complement(1315..2133)
                     /gene="nlpA"
                     /locus_tag="NBI74_003438"
     CDS             complement(1315..2133)
                     /gene="nlpA"
                     /locus_tag="NBI74_003438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpA"
                     /protein_id="HCD1933720.1"
                     /translation="MKLTTHHLRAGAALLLAGVLLAGCDQSSSDEKHIKVGVINGAEQ
                     DVAEVAKKVAKEKYGLDVELVGFSGSLLPNDATNHGELDANVFQHRPFLEQDNQAHGY
                     KLVAVGNTFVFPMAGYSKKIKTVAQIKEGATVAIPNDPTNLGRALLLLQKEKLITLKE
                     GKGLLPTALDITDNPRHLQIMELEGAQLPRVLDDPKVDVAIISTTYIQQTGLSPVNDS
                     VFIEDKNSPYVNILVAREDNKNAENVKEFLQSYQSPEVAKAAETIFNGGAVPGW"
     gene            2355..2648
                     /locus_tag="NBI74_003439"
     CDS             2355..2648
                     /locus_tag="NBI74_003439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1933721.1"
                     /translation="MKPTMLLMITIFLIFPAISQAESPFSSLQSAKEKTTVLQDLRKI
                     CTPQASLSDEAWEKLMLSDESNKQHIREAIVAMERNNQSNYWEALGKVECPDM"
     gene            complement(2689..3879)
                     /gene="nepI"
                     /locus_tag="NBI74_003440"
     CDS             complement(2689..3879)
                     /gene="nepI"
                     /locus_tag="NBI74_003440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine ribonucleoside efflux pump NepI"
                     /protein_id="HCD1933722.1"
                     /translation="MSEFIAENRGADAITRPNWSAVFSVAFCVACLIIVEFLPVSLLT
                     PMAQDLGISEGVAGQSVTVTAFVAMFASLFITQTIQATDRRYVVILFAVLLTLSCLLV
                     SFANSFSLLLIGRACLGLALGGFWAMSASLTMRLVPPRTVPKALSVIFGAVSIALVIA
                     APLGSFLGELIGWRNVFNAAAVMGVLCIFWIIKSLPSLPGEPSHQKQNTFRLLQRPGV
                     MAGMIAIFMSFAGQFAFFTYIRPVYMNLAGFGVDGLTLVLLSFGIASFVGTSLSSFIL
                     KRSVKLALAGAPFVLALSALVLTLWGSDKIVATGVAIIWGLTFALIPVGWSTWITRSL
                     ADQAEKAGSIQVAVIQLANTCGAAIGGYALDNIGLTSPLMLSGTLMLLTALLVTAKVK
                     MKKS"
     gene            4113..4466
                     /locus_tag="NBI74_003441"
     CDS             4113..4466
                     /locus_tag="NBI74_003441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HCD1933723.1"
                     /translation="MFKTKWFAREARSHAITDEELCRAILETEQGKADVLGGGVFKKR
                     LHRNRERAIILAKGGSNWFYTFLYAKQDMSNINSQELAGFRELAKHYAFLTKAQLTAM
                     INTKELTEICYDCKN"
     gene            4450..4773
                     /locus_tag="NBI74_003442"
     CDS             4450..4773
                     /locus_tag="NBI74_003442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HCD1933724.1"
                     /translation="MTAKTKFKSPAFEAIHSAAAGLSRVDAISAETMRTFDKACLTSV
                     QDLQPVEIKALREKLKVSQPVFAHYLNTSVSTVQKWESGAKRPNGISLKLLSIVQKHG
                     LEVLL"
     gene            complement(4889..5341)
                     /locus_tag="NBI74_003443"
     CDS             complement(4889..5341)
                     /locus_tag="NBI74_003443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1198 domain-containing protein"
                     /protein_id="HCD1933725.1"
                     /translation="MIWIMLATLAVVFVVGFRVLTSGARKAIRRLSDRLNIDVVPVES
                     MVDQMGKSAGDEFLRYLHRPDESHLQNAAQVLLIWQIVIVDGSEQNLLQWHRILQKAR
                     LAAPITDAQVRLALGFLRETEPEMQDINAFQMRYNAFFQPAEGVHWLH"
     gene            complement(5394..6728)
                     /gene="adeQ"
                     /locus_tag="NBI74_003444"
                     /gene_synonym="yicO"
     CDS             complement(5394..6728)
                     /gene="adeQ"
                     /locus_tag="NBI74_003444"
                     /gene_synonym="yicO"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeQ"
                     /protein_id="HCD1933726.1"
                     /translation="MNNDNTDYVSNESGTLSRLFKLPQHGTTVRTELIAGMTTFLTMV
                     YIVFVNPQILGAAQMDPKVVFVTTCLIAGIGSIAMGVFANLPVALAPAMGLNAFFAFV
                     VVGAMGISWQTGMGAIFWGAVGLFLLTLFRIRYWMISNIPLSLRIGITSGIGLFIALM
                     GLKNTGVIVANKDTLVMIGDLSSHGVLLGILGFFIITVLSSRHFHAAVLVSIVVTSCC
                     GLFFGDVHFSGVYSIPPDISGVIGEVDLSGALSLELAGIIFSFMLINLFDSSGTLIGV
                     TDKAGLIDSNGKFPNMNKALYVDSISSVAGAFIGTSSVTAYIESTSGVAVGGRTGLTA
                     VVVGVMFLLVMFFSPLVAMVPPYATAGALIFVGVLMTSSLARVNWDDFTESVPAFITT
                     VMMPFTFSITEGIALGFMSYCIMKVCTGRWRDLNLCVVVVAALFALKIILVD"
     gene            6903..8669
                     /gene="adeD"
                     /locus_tag="NBI74_003445"
     CDS             6903..8669
                     /gene="adeD"
                     /locus_tag="NBI74_003445"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HCD1933727.1"
                     /translation="MNNSINHKFHHISRAEYQELLAVSRGDAVADYIIDNVSILDLIN
                     GGEISGPIVIKGRYIAGVGAEYADAPALQRIDARGATAVPGFIDAHLHIESSMMTPVT
                     FETATLPRGLTTVICDPHEIVNVMGEAGFAWFARCAEQARQNQYLQVSSCVPALEGCD
                     VNGASFTLEQMLAWRDHPQVTGLAEMMDYPGVISGQNALLDKLDAFRHLTLDGHCPGL
                     GGKELNAYIAAGIENCHESYQLEEGRRKLQLGMSLMIREGSAARNLNALAPLINEFNS
                     PQCMLCTDDRNPWEIAHEGHIDALIRRLIEQHNVPLHVAYRVASWSTARHFGLNHLGL
                     LAPGKQADIVLLSDARKVTVQQVLVKGEPIDAQTLQAEESARLAQSAPPYGNTIDRQP
                     VSASDFALQFTPGKRYRVIEVIHNELITHSRSSVYSENGFDRDDVCFIAVLERYGQRL
                     APACGLLGGFGLNEGALAATVSHDSHNIVVIGRSAEEMALAVNQVIQDGGGLCVVRNG
                     QVQSHLPLPIAGLMSTDTAQSLAEQIDALKAAARECGPLPDEPFIQMAFLSLPVIPAL
                     KLTSQGLFDGEKFAFTTLEVTE"
     gene            complement(8716..10107)
                     /gene="uhpT"
                     /locus_tag="NBI74_003446"
     CDS             complement(8716..10107)
                     /gene="uhpT"
                     /locus_tag="NBI74_003446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexose-6-phosphate:phosphate antiporter"
                     /protein_id="HCD1933728.1"
                     /translation="MLAFLNQVRKPTLDLPLEVRRKMWFKPFMQSYLVVFIGYLTMYL
                     IRKNFNIAQNDMISTYGLSMTQLGMIGLGFSITYGVGKTLVSYYADGKNTKQFLPFML
                     ILSAICMLGFSASMGSGSVSLFLMIAFYALSGFFQSTGGSCSYSTITKWTPRRKRGTF
                     LGFWNISHNLGGAGAAGVALFGANYLFDGHVIGMFIFPSIIALIVGFIGLRYGSDSPE
                     SYGLGKAEELFGEEISEEDKETESTDMTKWQIFVEYVLKNKVIWLLCFANIFLYVVRI
                     GIDQWSTVYAFQELKLSKAVAIQGFTLFEAGALVGTLLWGWLSDLANGRRGLVACIAL
                     ALIIATLGVYQHASNQYIYLASLFALGFLVFGPQLLIGVAAVGFVPKKAIGAADGIKG
                     TFAYLIGDSFAKLGLGMIADGTPVFGLTGWAGTFAALDIAAIGCICLMAIVAVMEERK
                     IRREKKIQQLTVA"
     gene            complement(10245..11564)
                     /gene="uhpC"
                     /locus_tag="NBI74_003447"
     CDS             complement(10245..11564)
                     /gene="uhpC"
                     /locus_tag="NBI74_003447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312631.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter family glucose-6-phosphate
                     receptor UhpC"
                     /protein_id="HCD1933729.1"
                     /translation="MLPFLKAPADAPLMTDKHEIDACYRYWRRHILLTIWLGYALFYF
                     TRKSFNSAVPEILANGVLSRSDIGLLATLFYITYGVSKFVSGIVSDRSNARYFMGIGL
                     IATGIINILFGFSTSLWAFAVLWVLNAFFQGWGSPVCARLLTAWYSRTERGGWWALWN
                     TAHNVGGALIPIVMAASALHYGWRAGMMIAGCMAIVVGIFLCWRLRDRPQALGLPAVG
                     EWRHDALEIAQQQEGAGLTRKEILTKYVLLNPYIWLLSFCYVLVYVVRAAINDWGNLY
                     MSETLGVDLVTANTAVTMFELGGFIGALVAGWGSDKLFNGNRGPMNLIFAAGILLSVG
                     SLWLMPFASYVMQATCFFTIGFFVFGPQMLIGMAAAECSHKEAAGAATGFVGLFAYLG
                     ASLAGWPLAKVLDTWHWSGFFVVIAIAAGISALLLLPFLNAQTPREA"
     gene            complement(11574..13076)
                     /gene="uhpB"
                     /locus_tag="NBI74_003448"
     CDS             complement(11574..13076)
                     /gene="uhpB"
                     /locus_tag="NBI74_003448"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312632.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction histidine-protein
                     kinase/phosphatase UhpB"
                     /protein_id="HCD1933730.1"
                     /translation="MKTLFSRLITVIACFFIFSAAWFCLWSISLHLVERPDMAVLLFP
                     FGLRLGLMLQCPRGYWPVLLGAEWLLIYWLMQAVGLTHFSLLMIGSLLTLLPVALISR
                     YRHQRDWRTLLLQGAALTAAALLQSLPWLWHGKESWNALLLTLTGGLTLAPICLVFWH
                     YLANNTWLPLGPSLVSQPINWRGRHLVWYLLLFVISLWLQLGLPDELSRFTPFCLALP
                     IIALAWHYGWQGALIATLMNAIALIASQTWRDHPVDLLLSLLVQSLTGLLLGAGIQRL
                     RELNQSLQKELARNQHLAERLLETEESVRRDVARELHDDIGQTITAIRTQAGIVQRLA
                     ADNASVKQSGQLIEQLSLGVYDAVRRLLGRLRPRQLDDLTLEQAIRSLMREMELEGRG
                     IVSHLEWRIDESALSENQRVTLFRVCQEGLNNIVKHADASAVTLQGWQHDERLMLVIE
                     DDGSGLPPDSGQHGFGLTGMRERVTALGGTLTISCLHGTRVSVSLPQRYV"
     gene            complement(13076..13666)
                     /gene="uhpA"
                     /locus_tag="NBI74_003449"
     CDS             complement(13076..13666)
                     /gene="uhpA"
                     /locus_tag="NBI74_003449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator UhpA"
                     /protein_id="HCD1933731.1"
                     /translation="MITVALIDDHLIVRSGFAQLLGLEPDLQVVAEFGSGREALAGLP
                     GRGVQVCICDISMPDISGLELLSQLPKGMATIMLSVHDSPALVEQALNAGARGFLSKR
                     CSPDELIAAVHTVATGGCYLTPDIAIKLASGRQDPLTKRERQVAEKLAQGMAVKEIAA
                     ELGLSPKTVHVHRANLMEKLGVSNDVELARRMFDGW"
BASE COUNT         3762 a         3696 c         3330 g         3103 t
ORIGIN      
        1 ggaataaaac taaaaaatga gacaacatat gtttctcttc ctaatccaac cattttaata
       61 accacaacat caacacctga acgacagttt tctatttctg ttctggatga tgataacaaa
      121 gaaaatctac ctaccgatac cgcaccttgt tttataattg gtattcctga tgaatatact
      181 aaagccatag agttattgaa cattaaataa ttaaatttca tattgagttt ttagtgaggt
      241 tttttaaaca ccatatgaat aattgttata tgtcattatt tcctccattc actaaaactg
      301 attgattact ctactccctc accgtcatcg gtagtggtat ccttaacgta tattgatgaa
      361 aagacaaaac agtatgcaga gggaaaaatg ggttccacca gaaaggggat gctgaacgtt
      421 ctgattgccg ccgtgttgtg gggaagttca ggggtctgcg cgcaatacat catggagcaa
      481 agccagatgt cgtcgcagtt tttgactatg acgcgtttga tattcgccgg attgattctg
      541 ctgacgctat catttgttca tggtgataaa atcttttcta ttattaacaa tcacaaagat
      601 gccattagcc tactgatttt ttccgtggtt ggcgcgctaa ccgtacagct cacttttttg
      661 ctaaccatcg aaaaatcgaa cgcagccacg gcaacggtgc tgcaattcct ctcaccgact
      721 attatcgtcg cctggttctc actggtgcgt aaatcccgcc ccggcatcct ggttttttgc
      781 gctattttga catcgctgat cgggactttt ttattggtga cacacggtaa tccgacgtca
      841 ttatcgatct ctcctgccgc gttattctgg ggcattgcct cggcatttgc tgctgcattc
      901 tataccacct atccctcaac gctgattgct cgctatggca cgttaccggt cgtcggctgg
      961 agtatgctca ttggcggtct gatcctgctg cccttttatg ccagacaagg gacaaacttt
     1021 gtcgttaacg gcagtttgat tctggcgttt ttttatttgg tggtcattgg tacgtccctg
     1081 acatttagtc tgtacctgaa aggagcacaa ttaattggtg gtccaaaagc cagcatttta
     1141 agctgtgcag aaccattaag tagcgcactg ctctctttgc tgttgctggg gatcaccttc
     1201 acattgccgg actggctggg aacgctgctg attctgtcat cggtgatttt gatttcaatg
     1261 gattcccgcc gccgcgccag aaaaataaat cgtccggcgc ggcatgagtg agaattacca
     1321 gccaggcacc gcgcctccgt taaagatggt ttctgccgct ttagcgactt cgggtgattg
     1381 ataagattgc agaaattcct tcacgttttc tgcattctta ttatcttccc gtgccaccag
     1441 aatattcaca tacggcgaat ttttatcttc aataaatacg ctgtcgttaa ccggagaaag
     1501 tcctgtttgc tggatgtaag tggtgctgat aatcgctaca tccactttcg ggtcgtccag
     1561 tacacgtggc aactgtgccc cttccagctc cattatctgc aaatggcgtg gattatcggt
     1621 tatatccaac gcggtaggta ataacccttt tccctctttc agagtaatta gcttctcttt
     1681 ttgcagcagt aaaagcgcac gaccaaggtt agtcgggtcg ttggggatcg ccactgtcgc
     1741 gccttctttt atttgcgcca cggttttgat ttttttggaa taacccgcca tggggaagac
     1801 aaacgtattt cccaccgcta ccagtttata accatgcgcc tgattatctt gttcaagaaa
     1861 gggtcgatgc tggaagacgt tcgcatcaag ctcgccatga tttgtcgcat cgttcggtag
     1921 caacgaaccg ctaaatccta ccagttcaac atcgaggcca tatttctctt ttgctacctt
     1981 tttagcgact tccgcgacat cttgttctgc gccattaata acgccaactt taatatgctt
     2041 ttcatcgcta ctgctctggt cgcaacctgc cagcagaact cctgccagta ataatgcggc
     2101 ccccgcccgt agatgatgtg ttgtcagttt cattttttta tccttttatt aatgattacc
     2161 tgactatagc ggtaaggtcg ctgcagttta aaaaacgaaa cgctatcgat aagaataaaa
     2221 aggaataaaa gtggaatata aagtctggca cttcttactg accacgcaaa cacctttcgt
     2281 acagcatgat gagagcggca aaagcaaact acacttatgc ttcattcgat gcacatttgc
     2341 agaaggttgc cgctatgaag ccaacgatgc tactcatgat taccatattc ctgatttttc
     2401 cagccatttc tcaggccgag tcgccattca gttccctaca gtcggcaaaa gagaaaacca
     2461 cggtattgca agatttacgt aaaatttgta ccccgcaggc atcgttatca gatgaagcgt
     2521 gggaaaagtt aatgttgtct gatgaaagca ataaacaaca catacgcgaa gccatcgtag
     2581 cgatggaacg taataatcag agcaattatt gggaagcgtt gggtaaggta gaatgcccgg
     2641 atatgtaaga acggcaacaa ccactgacca acgcttcttt acctgatatc aggatttctt
     2701 cattttcacc tttgcagtca ccaacaatgc agtcagcaac atcaatgtgc cggacaacat
     2761 cagcggcgaa gtcagaccaa tgttatccaa cgcataaccg ccgattgccg caccgcaggt
     2821 attggcaagc tgaataacgg ccacctgaat agatccggct ttttctgcct gatcggccag
     2881 tgagcgagtg atccacgttg accagccaac gggaattaaa gcaaaggtca gcccccagat
     2941 aatcgccaca ccagtagcaa caattttatc gcttccccac agcgtcagta ccagcgcgct
     3001 cagggcgagc acaaacggtg cgcccgctaa ggccagtttc accgaacgtt tgagaataaa
     3061 cgacgaaagc gatgtcccga caaagctggc gataccaaaa ctcagcagca ccagggttaa
     3121 gccatcaaca ccgaatcccg ccagattcat atacactggg cgaatataag tgaaaaaagc
     3181 aaactgtccg gcaaaggaca taaagatggc gatcatcccg gccatcactc ccggacgttg
     3241 taataagcgg aaagtatttt gtttctgatg cgaaggttcg cctggcagcg aaggcagtga
     3301 tttgataatc cagaaaatac acagcacgcc catcaccgcc gccgcattaa agacattgcg
     3361 ccaaccgata agctcgccta aaaaactgcc caacggcgcg gcaatcacca gcgcaataga
     3421 aaccgcgccg aagatcaccg acagcgcctt cggcaccgta cgcggcggta ccagacgcat
     3481 ggtcagcgac gccgacatcg cccagaatcc gcccagcgcc agccccagac aggcgcgacc
     3541 gattaacaac agactgaatg agttggcaaa ggaaaccagc aaacaggaaa gcgtcagcaa
     3601 aacggcgaac agaatgacaa cgtagcggcg gtcagtggcc tgaatggtct gggtaataaa
     3661 caaactggca aacatcgcca caaaagcggt tacggtcacc gattgcccgg caaccccttc
     3721 cgaaatgcct aaatcctggg ccattggcgt caacaaactg acgggcaaaa actcaacgat
     3781 aataagacag gcaacgcaaa atgccaccga gaaaacggct gaccagttcg gtcgggtgat
     3841 ggcatccgcg ccgcggtttt cagcaataaa ttcactcatg gtgttacccg tggcatgttt
     3901 cagagggaaa gccgaccact ttaacattga aatgtgtgac gtatttaacg tttttgcaac
     3961 ttttacggtg ttgtgggaaa agttcgaggc tgctcgaaga aataaaaatc atagggcaag
     4021 gtgatatgca ttttcgcgat taggttggga ataagggata aagtattgca cttggtgata
     4081 tactttaata acaggatctc aacgatatgc gcgtgtttaa aaccaaatgg tttgcaaggg
     4141 aagccaggtc tcatgcaata acagatgaag agttgtgccg ggccatttta gaaacggaac
     4201 aaggtaaagc tgatgtactt ggtggtggcg tttttaaaaa gcgccttcac cgaaatcgtg
     4261 agcgtgcaat tattctggcg aaagggggaa gcaattggtt ttacacgttt ttatatgcaa
     4321 aacaggatat gtcgaacatt aatagtcagg aactggctgg gtttcgtgaa cttgcaaagc
     4381 attatgcctt cctgacgaaa gcccaactga cagcaatgat taacactaaa gaactgacag
     4441 agatctgcta tgactgcaaa aactaaattt aaaagtcctg catttgaagc tattcacagc
     4501 gcagcggctg gattgtccag agtcgatgct atttctgccg aaacgatgcg tacttttgac
     4561 aaagcctgcc tgaccagcgt gcaagactta caacctgttg agataaaagc actgcgggaa
     4621 aaattaaaag tgagtcagcc cgtcttcgct cactatctca ataccagcgt ttcaactgta
     4681 caaaaatggg aaagtggcgc taaacgcccc aatggcattt cattaaaact gctcagcatt
     4741 gtgcaaaaac acgggctgga ggtgctgctg taaatagaac gtagataatg cgttcaccta
     4801 ctgtcaattc cgataccgga tgcgacgtac acgccttatc cacccaccag gtcaggcgcg
     4861 gtctgtaggc tgataagacg aagcggtttt aatgcagcca gtgaacgccc tcggcaggct
     4921 gaaagaacgc gttatagcgc atctgaaaag cattgatatc ctgcatttca ggttcggttt
     4981 cgcgcagaaa acccagcgcc agtctgacct gagcatcggt aatcggcgcg gcaaggcggg
     5041 ctttttgtaa aatccgatgc cattgcagca ggttctgttc gctaccatcg acaatgacaa
     5101 tttgccagat gagcaacacc tgcgcggcgt tttgcaggtg cgactcatcc gggcgatgca
     5161 aataacgtaa aaattcgtca ccggctgact ttcccatttg atcgaccatc gactccacgg
     5221 gtacgacatc gatgttcagc cgatcgctga gacggcgaat cgcttttctg gccccggatg
     5281 tcagcacccg aaaaccaacc acaaacacta ccgccagcgt ggcgagcatt atccagatca
     5341 tgagttctcc tgcgtaatca gaactcatga tatctggaaa ccctcgccag ttactaatcc
     5401 accagaataa tcttcagtgc aaacagagct gcgaccacca ccacacacag gttcagatcg
     5461 cgccagcgcc cggtgcacac tttcatgatg cagtacgaca taaagcctag tgcaatccct
     5521 tcggtgatcg agaacgtaaa gggcatcatc accgtggtaa taaacgcagg caccgattcg
     5581 gtaaaatcat cccagttaac gcgtgccagg ctcgatgtca tcaatacgcc aacaaagatt
     5641 aacgctccgg cggttgcgta aggaggaacc atcgccacca gcggtgagaa gaacataacc
     5701 agcagaaaca taacgccgac aacaaccgca gtcagccccg tgcgaccacc gacagccaca
     5761 cctgaagtac tttcaatata ggcggtaaca gacgaggtac cgataaacgc acccgccacc
     5821 gaactgatgc tatcaacata cagcgcctta ttcatattgg ggaatttacc gttactatct
     5881 attaagccag ctttatcagt cacaccaatt aatgtccctg atgaatcaaa taagttgatc
     5941 agcataaagg agaaaatgat accggcgagt tcaagtgata acgcaccgct caaatccact
     6001 tcaccaatga cgccgctaat atcaggcgga atggaataga caccgctaaa atgaacatca
     6061 ccgaaaaata atccacagca agacgtcacc acaatagaaa caagcaccgc ggcatgaaaa
     6121 tgacgtgatg acaacacagt tataataaaa aaccctaaaa tacctaacaa cacgccgtga
     6181 gaacttaaat cgccaatcat caccagggtg tctttattgg cgacaataac gccagtattt
     6241 tttaatccca ttaaggcgat aaataatccg attccgctgg tgataccaat acgtaaactt
     6301 aatggaatgt tggagatcat ccagtaccgg atacgaaaca gcgtgagcaa aaatagccca
     6361 actgcgcccc agaatatcgc gcccatcccg gtctgccagg agatacccat cgcccccacg
     6421 accacgaagg caaagaaggc gttcaggccc attgccggag ccagtgccac gggtaagtta
     6481 gcaaataccc ccatcgcaat actgccgata ccggcaatca aacaggtggt aacaaacacc
     6541 acttttgggt ccatttgtgc cgcgccgagg atttgcgggt tcacaaaaac gatgtacacc
     6601 atggttaaaa aagtggtcat ccccgcaatc aattctgtgc ggacggtggt cccatgctga
     6661 ggtagtttaa ataatcgcga aagcgtccct gattcattac tcacgtaatc ggtattgtca
     6721 ttattcattt ttttgtccat tggaggagat ttaatccctt gcttttgtaa aacaaatgac
     6781 aacaacggtt cagtgataat tatcacattt caattgcaca ttaatggata ttctttaata
     6841 atctcgcgac gtttctttat gataaataat aatcaaattg ataaaatcaa aatgagaaaa
     6901 atatgaataa ttctattaac cataaatttc atcacattag ccgggctgaa taccaggaat
     6961 tgttagccgt ttcccgtgga gacgctgttg ccgattatat tattgataat gtctctattc
     7021 tcgacctgat caatggcgga gaaatttccg gcccaattgt gattaaagga cgttacattg
     7081 ccggtgttgg cgcagaatac gctgatgctc cggctttgca gcggattgat gcccgcggcg
     7141 caacggcggt gccagggttt attgatgctc acctgcatat tgaatccagc atgatgacgc
     7201 cggtcacctt tgaaactgct accctgccgc gcggcctgac gaccgttatt tgcgaccctc
     7261 atgaaatcgt caacgtgatg ggagaagccg gattcgcctg gtttgcccgc tgtgccgaac
     7321 aggcaaggca aaaccagtac ttacaggtca gctcttgcgt acccgccctg gaaggctgcg
     7381 atgttaatgg tgccagtttt acccttgaac agatgctcgc ctggcgggac catccgcagg
     7441 ttaccggcct tgcagaaatg atggactacc ctggcgtaat tagcgggcag aatgcgctgc
     7501 tcgataaact ggacgcattt cgccacctga cactggacgg tcactgcccg ggtttgggtg
     7561 gtaaagaact taacgcctat attgctgcgg gtattgaaaa ctgccacgaa agttatcagt
     7621 tggaagaagg acgccggaaa ttgcaactcg gcatgtcatt gatgatccgt gaagggtccg
     7681 cagcccgcaa tctcaacgcg ctggcaccgt tgatcaacga atttaacagc ccgcaatgca
     7741 tgctctgtac cgatgaccgt aacccgtggg agatcgccca tgaaggacac atcgatgcct
     7801 taattcgccg cctgatcgaa caacacaatg taccgctgca tgtggcatat cgcgtagcca
     7861 gctggtcgac ggcgcgccat tttggtctga atcacctcgg cttactggca cccggcaagc
     7921 aggccgatat cgtcctgttg agcgatgcgc gtaaggtcac ggtgcagcag gtactggtga
     7981 aaggcgagcc gatcgatgca caaaccttac aggcggaaga gtcggcgaga ctggcacaat
     8041 ccgccccgcc atatggcaat accattgatc gccagccagt ttccgccagt gactttgccc
     8101 tgcaatttac ccccggaaaa cgctatcgcg ttattgaggt aatccataac gaactgatca
     8161 cgcactcccg ctccagcgtc tacagcgaaa acggttttga tcgcgatgat gtgtgcttta
     8221 ttgccgttct tgagcgttac ggacaacgac tggctccggc ctgtggtttg ctcggcggct
     8281 ttggcctgaa tgaaggtgcg ctggcggcga cggtcagcca tgacagccat aatattgtgg
     8341 tgatcggtcg tagcgcagaa gagatggcgc tggcggtcaa tcaggtgatt caggatggcg
     8401 gcgggctgtg cgtggtccgt aacggccagg tacaaagtca tctaccgttg cccattgccg
     8461 ggctaatgag caccgacacg gcgcagtcac tggcggagca aattgacgcc ttgaaagccg
     8521 ccgcccgtga atgcggtccg ttaccggatg agccgtttat tcagatggcg tttctttccc
     8581 tgccagtgat ccccgcgctg aaactaacca gtcaggggct gtttgatggt gagaagtttg
     8641 ccttcactac gctggaagtc acggaataat aaaaaaaagc ccggcgtcat gccgggcaaa
     8701 agtcaccagt tacgtttatg ccactgtcaa ctgctgaatt tttttctcgc ggcggatttt
     8761 gcgttcttcc atcaccgcca ctatcgccat caggcagata caaccaatcg cggcgatatc
     8821 cagcgcggcg aaggtgcctg cccagccggt aaggccaaat accggcgtcc catcggcaat
     8881 cattcccaga cctaacttgg caaagctgtc accaatcagg taagcgaagg tgcctttaat
     8941 accatcggca gcgccaatcg cttttttagg tacaaagcca acagccgcca cgccaatcaa
     9001 cagttgcggg ccaaagacca ggaaacccaa cgcaaagaga gaagccaggt agatatattg
     9061 gttgctggcg tgttgataca caccgagcgt ggcgataatc agcgccagcg cgatacaggc
     9121 caccaggcca cggcgaccgt tcgccaggtc agagagccag ccccacagca gcgtaccgac
     9181 cagcgcacca gcttcaaaca gcgtaaagcc ctgaatcgcc accgctttag agagtttcag
     9241 ttcctggaac gcatacacgg ttgaccactg gtcgatacca atacgcacca catagaggaa
     9301 aatgttggcg aagcacagca gccagatcac tttgtttttc agcacatact caacaaagat
     9361 ctgccacttg gtcatatcgg tagattctgt ctctttatcc tcttcactga tctcctcgcc
     9421 aaacagctct tcagctttgc cgaggccata agattccggg gagtcgctgc cgtaacgcag
     9481 accgataaaa ccaacaatca gcgcgataat cgacgggaaa ataaacatgc cgatgacatg
     9541 accatcgaac aggtaatttg cgccgaacag cgccacacct gctgcgcctg caccaccaag
     9601 gttgtgagaa atgttccaga aaccgaggaa tgtcccgcgt ttacgacgcg gcgtccattt
     9661 ggtgatggtg gagtaactgc atgaaccgcc ggtactctgg aaaaagccgc tcaaggcgta
     9721 gaaggcaatc atcaggaaca ggctaaccga gccgctgccc atactggcac tgaagcccag
     9781 catacaaatg gcagacagga tcagcatgaa cggcaggaat tgtttggtgt ttttgccgtc
     9841 ggcgtagtaa gaaaccagcg ttttacccac gccataagtg atggagaagc ccaggccgat
     9901 catccccagc tgcgtcatgc tcaacccgta ggtcgagatc atgtcgttct gcgcgatgtt
     9961 aaagttcttg cgaatcaggt acatcgtaag gtagccgata aagaccacca ggtaggattg
    10021 catgaacggt ttgaaccaca ttttgcgccg cacttcgagc ggaaggtcca gggtcggctt
    10081 gcgaacctgg tttaagaaag ccagcatggg ttactcctga aatgaatacc tgcgtgaggc
    10141 atgcattgta aaaatcagca gcaaatcagg cctgagatag cgtccaggca aaacctggaa
    10201 aatttcttag ttttgccgga tatgaagtga aaaggtgaga tgcatcacgc ttctcgcggt
    10261 gtctgggcgt tcaaaaaggg cagtaacagc agtgcggaaa tcccggcggc gatggcgata
    10321 accacaaaaa atccgctcca gtgccaggta tcgagtactt tcgccagcgg ccaaccagca
    10381 agcgacgcac caagataagc aaacaagccg acaaaccccg tcgccgcccc tgccgcctct
    10441 ttgtgggaac actctgccgc cgccatgccg attaacatct gcgggccaaa gacaaaaaaa
    10501 ccaatggtga agaagcaggt tgcctgcatc acgtagctgg caaatggcat cagccacagc
    10561 gagccgactg aaagcaaaat tccggcggcg aaaatcaaat tcatcggccc gcggttgccg
    10621 ttaaacagtt tgtccgaacc ccaacccgct accagcgcac cgataaatcc gcccagttca
    10681 aacattgtca ctgccgtatt cgccgtgacc agatcgacgc ccagcgtctc ggacatatac
    10741 aaattgcccc agtcgttgat cgccgcccga accacataga ccagcacata gcaaaacgaa
    10801 agcagccaga tatacggatt cagcaacaca tatttggtga ggatctcttt acgcgtcagc
    10861 cctgcccctt cttgttgttg agcaatctcc agtgcgtcgt gccgccattc accaactgcc
    10921 ggtaaaccta acgcctgcgg gcgatcgcgc agccgccagc agagaaaaat ccccacgacg
    10981 atcgccatac aacccgcaat catcatcccg gcacgccagc cgtaatgcag cgcgctcgct
    11041 gccatcacaa tgggaatgag tgcgccgccg acgttatgcg ccgtgttcca taatgcccac
    11101 caaccgccgc gctcggtacg agaataccag gcggttaaca gacgcgcaca caccggtgaa
    11161 ccccagccct ggaaaaaggc gttcaacacc cagagcacgg caaacgccca tagcgacgtc
    11221 gagaagccaa acagaatgtt gataatgccc gtggcaataa gccctatccc cataaaataa
    11281 cgggcatttg agcgatcgct gacaatgccg gagacaaact tcgacacacc gtaggtaatg
    11341 taaaacaggg tcgctaacag gccaatatcg ctacggctga gcacgccgtt agcaaggatt
    11401 tctggtacgg cggagttaaa acttttacgc gtgaagtaaa acagcgcgta acccagccag
    11461 atggtcagca gaatatgccg acgccagtag cgatagcagg catcaatttc atgtttatca
    11521 gtcattaatg gcgcatcggc aggcgctttc agaaacggca acatcatcga accttagaca
    11581 taacgttgag gtagagaaac gctgacacgc gtgccgtgca ggcaggagat ggtcaatgtg
    11641 ccacccagcg ccgttacgcg ctcgcgcatt ccggtaaggc caaaaccgtg ttgcccggaa
    11701 tctggcggta aaccgctgcc atcgtcttca atcaccagca ttaaccgctc atcatgctgc
    11761 cagccttgca gcgtgaccgc gctggcatcg gcatgtttca caatgttgtt caacccttcc
    11821 tggcagacgc gaaatagcgt cacgcgctgg ttttcgctta acgctgattc atcgattcgc
    11881 cattcgagat gactgacaat gccgcgccct tccagctcca tttcccgcat cagtgagcgg
    11941 atggcctgct ccagggtgag atcatccagc tggcgcggac gtaaccgccc taacaagcgg
    12001 cgcaccgcgt cgtaaacgcc caacgacaac tgttcgatga gctgcccgct ctgtttcacg
    12061 ctggcgttat ccgccgcgag ccgctggaca atgccagcct gagtacgaat agcagtgatg
    12121 gtctgaccga tatcatcgtg cagctctcgc gccacatcgc ggcgcacgct ctcttcggtt
    12181 tccagcaacc gttcagcgag atgctgattg cgcgccagtt ccttttgcag cgactggtta
    12241 agttcacgca accgctggat gccagcgccc agtaacaacc ctgtcagact ttgcaccagc
    12301 agcgagagca ataaatccac cggatgatcg cgccaggttt gactggcgat cagcgcgatg
    12361 gcgttcatca atgtcgcaat cagcgctcct tgccaaccat agtgccaggc cagcgcgata
    12421 atcggcagcg ccagacagaa tggcgtaaag cgcgacagtt cgtctggcaa tcccaactgg
    12481 agccagagac tgataacaaa cagcagcaag taccagacca gatgtcgccc gcgccagttg
    12541 attggctgag aaaccagtga cgggccgagc ggcagccagg tgttattggc gagatagtgc
    12601 cagaacacca gacatatcgg ggccagcgtc aggccgccag ttaaagtcag cagcagcgca
    12661 ttccacgact ctttgccgtg ccaaagccag ggcaacgact gcaacaacgc cgccgccgtt
    12721 aacgccgcac cctgtaacag caaggtgcgc cagtcacgtt gatggcgata gcgcgagatc
    12781 agcgccacgg gcaataacgt cagtaaacta ccgatcatca ataaggagaa atgggttaaa
    12841 ccgaccgcct gcattagcca gtaaatcagc agccactccg cacccagtag caccggccag
    12901 tatccgcgcg ggcattgcag cattagccct agccgcaggc caaacggaaa taacagcacc
    12961 gccatatcag ggcgctcaac caggtgcagg ctgatactcc acaggcaaaa ccatgcggca
    13021 gagaagataa aaaagcaggc aataacggta attaaacggg agaacaacgt cttcatcacc
    13081 agccatcaaa catgcggcgc gccagttcaa cgtcgttact gacgcccagt ttttccatca
    13141 gattggcgcg atggacgtgt actgttttcg gcgacaagcc cagttcggcg gcaatctctt
    13201 tcaccgccat tccttgcgcc agtttttctg ccacctggcg ttcgcgtttg gttagcgggt
    13261 cctggcgacc agatgccagt ttaatggcaa tatccggcgt cagataacag ccgccagtgg
    13321 caaccgtatg caccgcagca atgagttcat ccgggctaca gcgtttggag agaaagccgc
    13381 gcgcccccgc gttaagcgcc tgctcaacca gcgccggact gtcgtgaacg gagagcataa
    13441 ttgtcgccat gcctttcggc agctggctta gaagctccag accggagata tcaggcatgg
    13501 aaatatcgca aatacacacc tgcacaccgc gccccggcag ccccgccagc gcctcgcgcc
    13561 ccgaaccaaa ctcggcaact acctgcaaat caggttccag ccccagcagc tgcgcaaagc
    13621 cggagcggac gataaggtga tcgtctataa gggcaacggt gatcatggtc ttgtcctggc
    13681 gggtaataaa acgcgcttac cttaacgata agcgcgatgt tgttcaagcc ttgagcggtt
    13741 actgccaaaa gctgcgctct tgttgcttgt ggaaagaagt gatgccttca aggacttgta
    13801 tatcgactaa ccattaaaaa tgctggcggc attaatacct tgttagtatc cattttgaat
    13861 aagtaaaatg tatattctat atttatagtt t
//
LOCUS       DAHHTG010000111        13733 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.112, whole genome shotgun sequence.
ACCESSION   DAHHTG010000111 DAHHTG010000000
VERSION     DAHHTG010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13733)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13733)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13733
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.112"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            133..1155
                     /locus_tag="NBI74_003450"
     CDS             133..1155
                     /locus_tag="NBI74_003450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter substrate-binding protein"
                     /protein_id="HCD1933732.1"
                     /translation="MSLTRRRFTQILASTLFLHHLPSFAQSVKFWASLTLPEAQHITR
                     IVSAGAPADLLLLAVAPEKMVGFSSFDFARQALIPLPEHIRQFPRLGRLAGRASTLSL
                     EGLMALHPDLVVDCGNTDETWISQARQVSEQTQIPWLLLNGKLEQSAEQLTTLGKTLG
                     EEHRAAEQANLASRFVGEAQAFATSPAANLSFYAARGPRGLETGLQGSLHTEAAELLG
                     LHNVAQIADRHGLTQVSMENLLRWQPDIILVQEAVTADFIRRDPLWQGVKAVAEQRIL
                     FLSGLPFGWLDAPPGINRLLGLRRLHAWLDPAINRQFKSDMQHYAQLFWHCSLSDADY
                     QKLVAS"
     gene            1155..2135
                     /locus_tag="NBI74_003451"
     CDS             1155..2135
                     /locus_tag="NBI74_003451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HCD1933733.1"
                     /translation="MRTVNGCILLLAAISITFAAVSGAYHLDMQQLLALILRQENVPV
                     QEQIVFWQIRVPRILAALFLGAALAGAGTTYQGMFRNPLVSPDILGVSAGAGLGACAA
                     ILWGLSIVYIQLYAFCGGLMVVAGVWLITRRVTRHDPILTLVLVGIALGTLCGAGISL
                     IKTLADPYTQLPSITFWLLGGLSTVTLRDLCYAAPIILIGSLPLFFLRWRMNLLTLSD
                     DEARSLGLNVTRLRFGLIVCATLITASTVAIAGIIGWVGLVVPHIARLLTGHNHQQLL
                     PMAMCTGAILLLLTDTLARSIGTTEIPLGILTAFVGAPFFLFLLLRGGRQ"
     gene            2132..2890
                     /locus_tag="NBI74_003452"
     CDS             2132..2890
                     /locus_tag="NBI74_003452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCD1933734.1"
                     /translation="MTLLAVRHASLGYSRHPVLRDVSFTLSQGTICCLLGANGCGKTT
                     LMRSILGVIPLLKGEILLDSLPVQTLSHRQRAQAIAWVPQAHDGIFAFSVLDMVLMGL
                     APTIGAFSVPGKQERLKATEQLEKLGILHLATRRWNTLSGGERQLALIARALVQQPRL
                     LLLDEPASSLDFGHQIQLLDTLAQLKNNGMTMLMSTHHPLHANAIADSIIQVEPDGRV
                     TQGLPTEQLTTKKLAALYRVSADQIHHHLSAIKH"
     gene            2900..3712
                     /locus_tag="NBI74_003453"
     CDS             2900..3712
                     /locus_tag="NBI74_003453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000903984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HCD1933735.1"
                     /translation="MLIDDIDFADLYLQQLRLAHRTEKTPDHWDQRAEKMAENCASPT
                     DSYLQQLTSKIDLQGAQTLFDMGCGPGTVSLALADKLTTIYGVDYSQGMLNVAARRAA
                     ALKADNVHWIQRAWEEDWSDLPRCDIAVASRSTLVADMRQAMSKLNNQARLRVYTTHL
                     VSTSFVSPAIQRAAGREVIELPNYIFALNVLYQMGIYAHVDFIRGQNCQQDNSTWERF
                     EQNVSWSLGALNDDERERLYRWYQQQDARALAPASRDWALIWWDSVPQETLR"
     gene            3709..4563
                     /locus_tag="NBI74_003454"
     CDS             3709..4563
                     /locus_tag="NBI74_003454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ModD protein"
                     /protein_id="HCD1933736.1"
                     /translation="MIFLSQAQIDALLLEDIQGGDLTTRALNIGHQHGYIEFFLRQGG
                     CISGISVACKMLTTLGLTIDDAVSDGSQANAGQRLIRAQGNAAALHQGWKAVQNVLEW
                     SCGVSDYLAQMLALLRERYPDGNIACTRKAIPGTRLLASQAILAAGGLIHRAGCAETI
                     LLFANHRHFLHDNQDWSGAINQLRRHAPEKKIVVEADTPKEAIAALRAQPDVLQLDKF
                     SPQQATEIAQIAPSLAPHCTLALTGGINLTTLKNYLDCGIRLFITSAPYYATPADIKV
                     SLQPAASI"
     gene            4589..6559
                     /locus_tag="NBI74_003455"
     CDS             4589..6559
                     /locus_tag="NBI74_003455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HCD1933737.1"
                     /translation="MRLKKRYLCTALSLAFTQQAVAAQESDTLTVWSSPVSSTTTTVL
                     DQPTMKALDKQNVAQALSVVPGVVLQKSGSRNEEQVKVRGFDSRQVPVYFDGVPIYVP
                     YDGNLDLARILTNNLGAVEVSKGYSSLLQGPNQMGGAINITTQKPTKPLEASLGYRQG
                     WSRSQDNAYDMHASFAASSDLGYLQVSGSQLKQDFLGLPHGVNNDIAGKHGKMINSSA
                     DDKRGIVKLGFTPRENDEYTLTYIKQDGEKDNPPYSGNSGQKSRYWQWPEYDKESFYY
                     QGTTQLNDRFTLKSRLYRDTFENTLMMYNSLADLKNKKGSYSHYSDYSDGAGLQLAAD
                     VRENDLLSFAVNWKDDVHREKGAPHAAYDRYEDRTWSLASEYQWAAADNVDVVAGISY
                     DWRDSVEAKKHEKDGSITHYDDNNQSAFNWQVMGKYHFANEDTLALSYYDRTRFPTLK
                     ERYTTSKPAYNQIAIVNPQLKPERARGVDLTWNGAFTHDWGFEVSVYYNRVSDAILSH
                     NIDADTIQNQNSGTVDYSGLDAGIKGKISNILDVGLSYALIHADAKRKDIGKITDLPT
                     QTMTAWMTLKPWEPLSVTLSEEARSSSYSNSDGSQKAAGFAVTHIRADYTLGHGFSVN
                     ASVNNLFDTKYAYSEGFIEEGRNFWAGIEYTF"
     gene            complement(6609..6863)
                     /locus_tag="NBI74_003456"
     CDS             complement(6609..6863)
                     /locus_tag="NBI74_003456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HCD1933738.1"
                     /translation="MGIIAWIIFGLIAGIIAKLIMPGRDGGGFFLTCILGIVGAVVGG
                     WLATMFGIGGSISGFNLHSFLVAVVGAILVLGVFRLLRRE"
     gene            7064..7798
                     /locus_tag="NBI74_003457"
     CDS             7064..7798
                     /locus_tag="NBI74_003457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar brake protein"
                     /protein_id="HCD1933739.1"
                     /translation="MSHYHEQFLKQNPLAVLGVLRDLNKAAIPLRISWNGGQLISKIL
                     AITPDKLVLDFGSQAEDNIAVLKAQHITITAETQGAKVEFTVEQLQQSEYLQLPAFIT
                     VPPPTLWFVQRRRYFRITAPLHPPYFCQTKLADNSTLRFRLYDLSLGGMGALLETAKP
                     AELHEGMRFAQIEVNMGQWGVFHFDAQLISISERKVIDGKNETITTPRLSFRFLNVSP
                     TVERQLQRIIFSLEREARKKADNVRD"
     gene            complement(7800..8411)
                     /gene="emtA"
                     /locus_tag="NBI74_003458"
     CDS             complement(7800..8411)
                     /gene="emtA"
                     /locus_tag="NBI74_003458"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     EmtA"
                     /protein_id="HCD1933740.1"
                     /translation="MKLRWFAFLIVLLAGCSSKHDYTNPPWNAKVPVQRAMQWMPISQ
                     KAGAAWGVDPQLITAIIAIESGGNPNAVSKSNAIGLMQIKASTSGRDVYRRMGWSGEP
                     TTSELKNPERNISMGAAYLNILETGPLAGIEDPKVLQYALVVSYANGAGALLRTFSSD
                     RKKAISKINDLDADEFLDHVARNHPAPQAPRYIYKLEQALDAM"
     gene            8511..9425
                     /gene="ldcA"
                     /locus_tag="NBI74_003459"
     CDS             8511..9425
                     /gene="ldcA"
                     /locus_tag="NBI74_003459"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muramoyltetrapeptide carboxypeptidase"
                     /protein_id="HCD1933741.1"
                     /translation="MSLFHLIAPSGYCIKQHAALRGIQRLTDAGHQVNNVEVIARRCE
                     RFAGTETERLEDLNSLARLTTPNTIVLAVRGGYGASRLLADIDWQALVARQQHDPLLI
                     CGHSDFTAIQCGLLAQGNVITFSGPMLVANFGADELNAFTEHHFWLALRNKTFTIEWQ
                     GEGPTCQTEGTLWGGNLAMLISLIGTPWMPKIENGILVLEDINEHPFRVERMLLQLYH
                     AGILPRQKAIILGSFSGSTPNDYDAGYNLESVYAFLRSRLSIPLITGLDFGHEQRTVT
                     LPLGAHAILNNTEEGTQLTISGHPVLKM"
     gene            9520..11256
                     /locus_tag="NBI74_003460"
     CDS             9520..11256
                     /locus_tag="NBI74_003460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HCD1933742.1"
                     /translation="MDATTIISLFILGSILVTSSILLSSFSSRLGIPILVIFLAIGML
                     AGVDGVGGIPFDNYPFAYMVSNLALAIILLDGGMRTQASSFRVALGPALSLATLGVLI
                     TSGLTGMMAAWLFNLDLIEGLLIGAIVGSTDAAAVFSLLGGKGLNERVGSTLEIESGS
                     NDPMAVFLTITLIAMIQQHESSVSWMFVVDILQQFGLGIVIGLGGGYLLLQMINRIAL
                     PAGLYPLLALSGGILIFALTTALEGSGILAVYLCGFLLGNRPIRNRYGILQNFDGLAW
                     LAQIAMFLVLGLLVNPSDLLPIAIPALILSAWMIFFARPLSVFAGLLPFRGFNLRERV
                     FISWVGLRGAVPIILAVFPMMAGLENARLFFNVAFFVVLVSLLLQGTSLSWAAKKAKV
                     VVPPVGRPVSRVGLDIHPENPWEQFVYQLSADKWCVGAALRDLHMPKETRIAALFRDN
                     QLLHPTGSTRLREGDVLCVIGRERDLPALGKLFSQSPPVALDQRFFGDFILEASAKYA
                     DVALIYGLEDGREYRDKQQTLGEIVQQLLGAAPVVGDQVEFAGMIWTVAEKEDNEVLK
                     IGVRVAEEEAES"
     gene            complement(11313..12383)
                     /gene="dadX"
                     /locus_tag="NBI74_003461"
     CDS             complement(11313..12383)
                     /gene="dadX"
                     /locus_tag="NBI74_003461"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309712.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolic alanine racemase DadX"
                     /protein_id="HCD1933743.1"
                     /translation="MTRPIQASLDLQALKQNLSIVRQAAPHARVWSVVKANAYGHGIE
                     RIWSALGATDGFALLNLEEAITLRERGWKGPILMLEGFFHAQDLEIYDQHRLTTCVHS
                     NWQLKALQNARLKAPLDIYLKVNSGMNRLGFQPDRVLTVWQQLRAMANVGEMTLMSHF
                     AEAEHPDGISSAMARIEQAAEGLECRRSLSNSAATLWHQEAHFDWVRPGIILYGASPS
                     GQWRDIANTGLRPVMTLSSEIIGVQTLKAGERVGYGGRYTARDEQRIGIVAAGYADGY
                     PRHAPTGTPVLVDGVRTMTVGTVSMDMLAVDLTPCPQAGIGTPVELWGKEIKIDDVAA
                     AAGTVGYELMCALALRVPVVTV"
     gene            complement(12393..13691)
                     /gene="dadA"
                     /locus_tag="NBI74_003462"
     CDS             complement(12393..13691)
                     /gene="dadA"
                     /locus_tag="NBI74_003462"
                     /EC_number="1.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid dehydrogenase"
                     /protein_id="HCD1933744.1"
                     /translation="MRVVILGSGVVGVASAWYLNQAGHEVTVIDREPGAALETSAANA
                     GQISPGYAAPWAAPGVPLKAIKWMFQRHAPLAVRLDGTQFQLKWMWQMLRNCDTSHYM
                     ENKGRMVRLAEYSRDCLKALRAETNIQYEGRQGGTLQLFRTEQQYENATRDIAVLEDA
                     GVPYQLLESSRLAEVEPALAEVAHKLTGGLQLPNDETGDCQLFTQNLARMAEQAGVKF
                     RFNTPVDQLLCDGEQIYGVKCGDEVIKADAYVMAFGSYSTAMLKGIVDIPVYPLKGYS
                     LTIPIAQEDGAPVSTILDETYKIAITRFDNRIRVGGMAEIVGFNTELLQPRRETLEMV
                     VRDLYPRGGHVDQAAFWTGLRPMTPDGTPVVGRTRFKNLWLNTGHGTLGWTMACGSGQ
                     LLSDLLSGRTPAIPYEDLSVARYSRGFTPSRPGHLHGAHS"
BASE COUNT         3185 a         3695 c         3536 g         3317 t
ORIGIN      
        1 tcttgtggaa caaagtgtta tttaagaaac aagcaaatca ttctgtctct ttagacaaat
       61 tttgacatca aacatgatct ttttcgctat atgtgtaagt atatagcggt tttaattatc
      121 aggatattac ttatgtcact aaccagacgg aggtttacac agattcttgc gtcgacattg
      181 ttcctgcatc atctgccgtc ctttgcacaa tcagtcaaat tctgggcctc actgacgctt
      241 cccgaagccc aacacattac acgcatcgtc agcgcaggcg cgcccgccga tttactattg
      301 ctggctgtcg cgccagaaaa aatggtcggt ttttcctctt ttgattttgc ccgtcaggca
      361 ttaattccat tgccagagca cattcgccag ttccccaggc tgggacgact cgccgggcgc
      421 gccagcacac tctcgctgga agggcttatg gcgttacatc ccgatttggt cgttgattgc
      481 ggcaatacgg atgaaacctg gatctcccag gcacggcagg ttagcgaaca gacacaaata
      541 ccctggttat tgcttaacgg gaaactggaa caatcagcag aacagttaac aacgcttggc
      601 aaaacgttag gcgaagagca ccgcgccgca gaacaagcca atctcgccag ccgcttcgtt
      661 ggtgaagctc aggcattcgc cacctcaccc gccgctaatc tcagctttta tgctgcgcgc
      721 ggtcctcgag ggctggaaac gggcttacag ggttcgttgc ataccgaggc ggcggaatta
      781 ttaggtttgc acaacgtcgc gcaaatagcc gatcgccacg gtctgacaca ggtttccatg
      841 gaaaatctcc tgcgctggca gccggatatt attctggttc aggaggccgt tactgcagat
      901 tttattcgtc gtgatcctct ctggcagggc gtgaaagcgg ttgcggaaca acgcatctta
      961 tttttaagtg gcctgccctt tggctggctg gatgccccgc cgggaatcaa ccgtcttctg
     1021 ggattacgca gacttcacgc ctggctggat cccgccatca atcgccagtt taaaagtgac
     1081 atgcagcatt acgcccaact gttctggcat tgttcactca gtgacgccga ctatcaaaaa
     1141 ttggtggcga gctaatgaga accgtaaacg gatgtatttt gctgctggca gctatcagta
     1201 tcacttttgc cgccgtaagt ggggcttatc atctcgatat gcagcaactg ctggcgctca
     1261 ttcttcgtca agaaaatgtt cctgtgcagg aacaaattgt cttctggcaa atccgcgtgc
     1321 cgcgtattct tgctgcgctg tttctgggcg cggcgctggc aggggccgga accacctatc
     1381 agggaatgtt ccgtaatccc ttagtgtcac cggatatttt aggcgtctcc gccggtgccg
     1441 gacttggtgc ctgcgcggca atcctgtggg gattatccat tgtttacatc cagctgtacg
     1501 cattttgcgg cgggctgatg gtggtcgcgg gcgtctggtt gattacgcgt cgggtgaccc
     1561 gccacgatcc gatcttaacc ctggtgctgg taggcattgc gttaggaacg ttgtgcggag
     1621 cggggatatc gctaataaaa acgctggccg atccttacac ccagttaccg tcaattacct
     1681 tctggttgct gggggggctg tccaccgtga ccttacgcga tctctgctat gctgcaccga
     1741 tcattctcat aggctccttg cccctgtttt ttctccgctg gcgcatgaat ttactcacgc
     1801 tttccgacga cgaagcccgt tcgctgggac tgaacgtgac acgtctgcgc tttggattga
     1861 ttgtctgcgc cacactcatc accgccagca cggtagcaat agcaggcata attggttggg
     1921 tcggcctggt cgttccgcac attgcccgct tgttgaccgg tcataaccat cagcagctgt
     1981 tgcccatggc aatgtgtact ggcgcgattc tgctcctgct taccgatacg ctggcacgca
     2041 gcattggaac aacagaaatc ccacttggca ttctgaccgc ttttgttggt gcaccgtttt
     2101 tcctgttttt gttgttacgc ggaggtcgcc agtgactttg cttgccgtcc gtcatgcgtc
     2161 tctgggttac tcccgccatc cggtgctacg cgacgtgtcc ttcaccctgt cgcaaggaac
     2221 aatttgttgt ctgttaggag ccaatggttg cggcaaaacc acactcatgc gctccattct
     2281 tggcgttata ccgttattaa aaggcgagat cttactggat agcctccctg tgcagacact
     2341 cagccatcgc cagcgcgcgc aggctattgc ctgggtgccg caggcgcacg acggcatttt
     2401 tgcttttagt gtgctggata tggtgttgat gggcctggcc cccaccatag gcgctttttc
     2461 cgttcccggt aagcaagaac gcctgaaagc aacggagcaa ctggaaaagc tgggcatttt
     2521 gcatctggcg acacgacgct ggaatacgct tagcggcggt gaacgtcagc tggctttaat
     2581 cgcccgtgca ctcgtccagc aaccgcgctt gttattactg gatgagcctg cctccagcct
     2641 cgatttcggt catcagatcc aacttctgga cacgctggcg cagctcaaaa ataacggcat
     2701 gacaatgtta atgtcgactc atcatccgct ccatgcaaac gccattgccg acagcattat
     2761 ccaggtagag ccagatggac gagtaacaca aggcttaccg accgaacagt taacaacgaa
     2821 aaaactggct gccctgtacc gggtttctgc cgaccagatt caccatcacc tctctgcaat
     2881 taagcactaa ggaaaaatca tgctcattga cgatattgat tttgcagacc tttaccttca
     2941 gcaactcagg ctggcccatc gcacagaaaa aacgccggat cactgggatc aacgggcgga
     3001 aaaaatggcg gaaaattgcg cctcgcctac ggacagctac ctgcaacaac tgacctcaaa
     3061 aattgacctg caaggcgcgc aaaccttatt cgatatgggc tgcggaccgg gtaccgtatc
     3121 gctggcgctg gcggacaaac tcacgacgat ttatggcgtt gattacagtc aggggatgct
     3181 gaacgttgcc gcccgtcgtg ccgccgcgct aaaggcagac aatgtccact ggatccagcg
     3241 cgcctgggaa gaggactgga gtgacctgcc acgctgcgat attgccgtgg cttcccgctc
     3301 cactctggtg gctgatatgc gccaggcaat gagcaaactg aataatcagg cacggcttcg
     3361 cgtctatacg actcatctgg tcagcacctc gttcgtctct ccagccattc agcgggcggc
     3421 tggtcgggaa gtgatcgaat tacccaacta tatctttgca ctcaacgtcc tgtatcagat
     3481 gggaatttat gctcacgttg attttatccg cggacaaaat tgccaacagg ataacagcac
     3541 ctgggaacgt tttgagcaga acgtcagctg gagtctgggc gcgcttaacg acgatgagcg
     3601 tgagcggtta tatcgctggt atcaacaaca agatgctcgt gccctcgccc ctgccagccg
     3661 cgactgggcg ctcatctggt gggatagcgt accgcaggag acactgcgat gattttcctt
     3721 tctcaggcac aaatcgatgc gttattgctg gaagatatcc agggcggtga cctgaccacg
     3781 cgagcattaa atattggaca ccagcatggc tatatagagt tttttctccg tcagggcggc
     3841 tgcatcagcg gcatttctgt cgcgtgtaag atgttaacta cgctgggatt aactattgat
     3901 gacgcggtca gcgacggttc acaagcgaac gcgggtcagc ggctaatccg tgcgcaaggt
     3961 aatgccgcag cacttcatca gggatggaag gcagtccaga atgtgctgga gtggagttgc
     4021 ggtgtttctg attatctcgc tcaaatgctg gcgttacttc gtgaacgtta ccctgatggc
     4081 aatatcgcct gcacccgaaa agcaattccg ggcacccgct tactggcctc gcaggcaatt
     4141 ctggctgccg gaggactgat tcatcgtgcc ggatgtgcgg aaaccatatt attgtttgcc
     4201 aaccaccgcc attttcttca tgacaatcag gactggtcag gcgcaatcaa tcagttacgt
     4261 cgccacgcac ccgagaagaa aatagttgtt gaagccgaca cgccgaaaga ggcaatcgcc
     4321 gcgttacgcg cgcaaccaga cgtacttcag ctcgacaaat ttagtccgca acaggcaact
     4381 gaaattgcgc aaatagcacc gtcgctggct ccccactgca cgctggctct gaccggcgga
     4441 attaatctga caacactcaa aaattacctc gactgcggca ttcgcctttt tattacctcc
     4501 gcgccttatt acgcgacacc agctgacatt aaagtcagtc tgcaacccgc agcctctatt
     4561 taattctttg ttaaacggaa tttattctat gagactcaaa aaacgttatc tctgcacagc
     4621 attatcactt gcctttaccc agcaggccgt agcggctcag gagagtgaca cgctgaccgt
     4681 atggtccagt ccggtatcat caacgacgac caccgttctc gatcaaccca ccatgaaggc
     4741 cctggataaa cagaatgtcg ctcaggcatt aagtgtcgtc cccggcgtgg tgctgcaaaa
     4801 gtcaggtagc cgcaacgaag aacaggtcaa agttcgtggc tttgatagtc gtcaggtgcc
     4861 ggtctatttc gacggtgtgc ccatttatgt tccctatgac ggcaacctcg atctggcgcg
     4921 gattctgacc aacaatttgg gggcagttga agtttccaaa gggtattcgt cgctgcttca
     4981 ggggcctaat cagatgggcg gagccattaa tatcaccacc cagaagccaa caaaacctct
     5041 ggaagcaagt ctgggatatc gccagggatg gagccgtagc caggacaatg cctacgatat
     5101 gcatgcttca tttgccgcca gcagcgatct ggggtatttg caagtcagcg gtagccagct
     5161 aaagcaggat tttctcggcc tgccgcatgg tgtaaataat gatattgcag gcaaacacgg
     5221 caagatgatt aattcatcgg ctgatgataa acgcggcatt gtgaagctcg gttttacacc
     5281 acgcgaaaac gatgaataca cattgactta cattaagcag gatggtgaaa aagataaccc
     5341 gccatacagt ggaaatagtg gtcagaaatc acgctactgg cagtggccag agtatgacaa
     5401 agaaagtttt tattatcagg gaacgaccca actaaacgat cgttttaccc tgaaaagtcg
     5461 gctgtatcgc gacacctttg aaaatacgct aatgatgtac aactcgctgg ctgatttgaa
     5521 aaataaaaaa ggcagctaca gccattattc cgattacagc gacggtgccg ggttacaact
     5581 ggcagccgat gtgcgtgaaa acgatctgct gtcgtttgcc gttaactgga aagatgacgt
     5641 acaccgggaa aaaggtgcgc cgcacgccgc ttacgatcgc tatgaagatc gtacctggtc
     5701 gctcgccagt gaatatcaat gggctgctgc cgataatgtc gatgtcgtgg ctggaatcag
     5761 ctatgactgg cgcgatagcg tagaagcgaa aaaacatgag aaagatggca gtatcaccca
     5821 ttatgacgac aacaatcagt cagcttttaa ctggcaggtg atggggaaat accactttgc
     5881 caatgaagac acgctggcgc tttcgtacta tgaccgcaca cgctttccga cgctgaaaga
     5941 acgctatacc acgtccaaac ctgcgtataa ccagatagcg attgttaacc cgcagctcaa
     6001 gccggaacgc gcgcgcgggg tggatttaac ctggaatggt gccttcacgc acgactgggg
     6061 atttgaggtc agcgtttact ataaccgggt gagtgatgcc atcctctcgc acaatatcga
     6121 tgccgatacc attcaaaatc agaacagcgg cacggtggat tacagcggtc tggatgccgg
     6181 tattaagggg aaaatcagca atatactgga tgtaggattg agctacgccc tgatccacgc
     6241 tgatgccaaa cgcaaagaca tcggcaagat aaccgatctg ccaacgcaga caatgaccgc
     6301 atggatgact ctcaaaccgt gggagccgtt aagcgtaacg ctgtcggaag aggcgcgttc
     6361 ctccagctac agcaacagtg acggttcaca aaaagccgcc ggttttgcgg tgactcacat
     6421 tcgagccgat tacaccttag gtcatggctt cagcgttaat gcgtcggtca ataacctgtt
     6481 tgataccaaa tatgcctaca gtgaagggtt tattgaagaa ggtcgcaatt tctgggcagg
     6541 tatcgagtat acgttctgat gtgaaagcgg atagcggcat ctgtatcgct atccgcctta
     6601 tgaaaatctt attctcttcg caggaggcgg aatacgccca gcaccaggat agcgcccacc
     6661 accgccacca ggaagctgtg caaattaaaa ccgctgatgg agccgccaat gccaaacatg
     6721 gtcgccagcc agccgccgac caccgcaccg actatcccaa gaatacaggt caggaaaaat
     6781 ccaccaccat ctcgccccgg catgattagc ttggcgataa tgccggctat caggccaaaa
     6841 ataatccagg caataattcc catgttcagg ccctcgtttc gatgaatcgg acgcaagaaa
     6901 tgcttacgtc aacgctctaa gtataggcaa gggcgggatc gatgctgggc atatgtcggc
     6961 tgatttaaga acaacatgta aaaaatattc gcgcatttgt attaagtttt gttaactgcg
     7021 accgataaac caaagacagt ttgtcagtca ggagtttttc cgcgtgagtc attaccatga
     7081 gcagttcctg aaacaaaatc cgttagccgt cctgggcgtg ttacgcgatt tgaacaaagc
     7141 cgcaattcct ttgcgtatca gctggaatgg cgggcagctg atcagcaaaa tactggcaat
     7201 aactccggat aaactggtgc tggatttcgg cagccaggcc gaagacaaca tcgccgtgct
     7261 aaaggcgcag cacattacca ttaccgccga aactcagggt gcgaaagtcg agtttactgt
     7321 tgaacaacta cagcagagtg aatacttgca gcttccggca tttattaccg taccgcctcc
     7381 caccttatgg tttgtacaac gacgccgata tttccgcatc accgccccac tccatccgcc
     7441 ttatttttgc cagaccaaac tggcggataa cagtacgtta cgtttccgcc tgtatgattt
     7501 gtcgttaggc ggcatgggcg cattactgga aacagcaaag cctgccgaat tacacgaagg
     7561 catgcgcttc gctcagattg aagtcaacat ggggcaatgg ggtgtttttc actttgacgc
     7621 ccagttaatc tccatcagcg agcgcaaagt gattgatggc aaaaatgaaa ccatcaccac
     7681 tccccgtctg agcttccgtt ttcttaacgt cagcccgacg gtggagcggc aattacagcg
     7741 gattattttc tctctcgagc gagaagcccg gaaaaaagcg gacaatgtgc gtgactgatt
     7801 tacatcgcgt ccagtgcctg ctcaagtttg tagatatagc gcggagcctg cggcgcagga
     7861 tgatttcgcg ctacgtgatc gaggaactca tcagcatcca gatcgttgat tttgctgatc
     7921 gcctttttcc gatctgacga gaaagtccgt agcagcgcac ctgccccgtt agcgtatgac
     7981 accaccagcg catattgcag taccttcgga tcttcaatgc ctgccagcgg gccggtttcc
     8041 agaatattca ggtaagccgc tcccattgaa atattacgct ccggattttt cagctcgctg
     8101 gtcgtcggct caccgctcca gcccatacga cgatatacat cacgtccgga ggttgaagct
     8161 tttatctgca tcaaaccaat ggcattcgat ttactcaccg cgttcggatt accacccgat
     8221 tcgatagcga taatcgccgt gatcaattgt ggatcgacgc cccaggctgc accggctttc
     8281 tggcttattg gcatccactg catcgcacgt tgcaccggaa ctttcgcgtt ccacggcggg
     8341 ttcgtatagt catgctttga tgaacaaccc gctaataaca caatcaaaaa ggcaaaccat
     8401 ctcaatttca cttcttctat ccttatagcc tggaatcgcc gttgaggtga caacggcaca
     8461 cctaagcggc atgatataca tttcgtttct gttgtcagca aggaattgcc atgtctctgt
     8521 ttcacttaat tgccccatcg ggttactgca ttaaacagca cgccgcgctg cgtggtatcc
     8581 agcgccttac cgacgcgggg catcaggtca ataacgtcga ggttattgcc cgtcgttgtg
     8641 agcgttttgc gggtacggaa acggagcgtc tggaagatct caattctctt gccagactga
     8701 ctacccccaa caccatcgtg ctggctgtac gcggcggtta cggtgccagt cgtttactgg
     8761 cagatattga ctggcaggca ctggtggctc gccaacaaca tgacccgtta ctcatttgcg
     8821 gacatagcga ttttaccgcc attcagtgcg gtcttctggc gcaaggcaat gtcatcacct
     8881 ttagcggccc catgctggta gcaaactttg gcgcggatga actaaatgcc tttaccgagc
     8941 accacttctg gctggcgtta cgcaataaaa cgttcaccat tgaatggcaa ggggaaggcc
     9001 cgacatgtca gaccgaaggc acgctgtggg gaggcaatct tgcgatgctg atttcactga
     9061 ttggtacacc gtggatgcca aaaattgaga acggtattct ggtgctggaa gatattaacg
     9121 agcacccttt ccgcgtcgaa cgcatgctgt tgcagctcta tcatgctgga attttgccac
     9181 gtcagaaggc gattattctc ggtagcttta gcggcagcac acctaatgat tatgacgcag
     9241 gttacaacct ggagtcagtg tacgcgtttt tgcgttcccg cctgtcgatt ccactcatta
     9301 ccggcctcga ttttggtcat gaacaacgca cggttacgct gccgttgggc gcacatgcca
     9361 ttctgaataa caccgaggaa ggcactcagt tgacgattag cggtcatcct gttcttaaaa
     9421 tgtaaaaaaa tcacgcttca gggctaagta aaacgctgtc tctgccgcta atatggtagg
     9481 gtttatatca gagacagcgt aatcaggagt aaccgaccgt tggatgccac aacaataatt
     9541 agccttttca tcttaggatc catccttgtc accagcagta tattacttag ttcattttct
     9601 tcccgtcttg gcattcctat tctggttatt tttctggcga tcggcatgtt ggcgggggtt
     9661 gatggcgtcg gcggtatccc gtttgataat taccccttcg cctacatggt aagtaacctg
     9721 gcgctggcga ttattttgct cgacggcggg atgcgcactc aggccagttc ctttcgtgtg
     9781 gcgttaggac cggcactgtc gctggcgacg ctgggcgtac ttatcacctc tggtttaacc
     9841 ggcatgatgg cggcgtggct gtttaatctt gatttgattg aaggattatt aatcggcgct
     9901 atcgtcggct ccaccgatgc tgcagcggtc ttttctctgc tgggtggtaa ggggcttaac
     9961 gaacgtgttg gctcgacgct ggaaattgaa tccggcagta atgatccaat ggcggtcttt
    10021 ctgacgatta ctctgattgc gatgatccag cagcatgaaa gcagtgtcag ctggatgttc
    10081 gtggtcgata ttctgcagca atttggcctc gggattgtca tcgggcttgg cggtggttat
    10141 ttactgctgc aaatgattaa tcgcatcgcc ctgcccgccg gattatatcc attgctggca
    10201 ttaagcggcg gtattttaat ttttgcttta accacggcgc tggaaggcag cggtattctg
    10261 gctgtttatc tgtgcggttt tctgctgggt aatcggccga ttcgcaaccg ctacggcatc
    10321 ctgcaaaatt tcgacggcct cgcctggctg gcgcaaatcg ccatgttcct ggtgctgggg
    10381 ctattggtta acccaagcga tctgctgcct attgccattc cggcgctcat tttgtccgca
    10441 tggatgattt tcttcgcccg tcctctttcg gtatttgccg gattgctacc gttccgtggc
    10501 ttcaatctgc gtgagcgcgt gtttatcagc tgggtaggat tacgcggcgc ggtgccgatt
    10561 attctggctg tgttcccgat gatggcgggg ctggagaatg cgcgactgtt ctttaatgtc
    10621 gccttctttg tggttctggt ttcactgcta ttgcagggaa catcactctc gtgggcggcg
    10681 aaaaaagcca aagtggtcgt tccgccagtg ggacgtccgg tgtcacgcgt tggcctggat
    10741 attcatccgg aaaatccgtg ggagcagttt gtttatcaat tgagtgccga taaatggtgc
    10801 gtgggcgcgg cactgcgtga tttgcatatg ccaaaagaga cgcgtattgc ggcactgttt
    10861 cgtgataacc agttgcttca tcccaccggc agcacccgac tgcgcgaagg cgatgtgttg
    10921 tgtgtaattg gtcgggaacg cgatctcccg gcgctcggta aactgttcag ccagtcgccg
    10981 ccggtcgcgc tggatcaacg cttctttggt gacttcattc tcgaagccag cgccaaatat
    11041 gctgatgtgg cgctgatata tggtctggaa gacgggcgag aatatcgcga taagcagcaa
    11101 acgctgggtg aaatcgtcca gcagttgtta ggcgcggcac cggttgtcgg tgaccaggta
    11161 gagtttgccg ggatgatctg gacggtggcc gagaaagaag ataatgaagt gttgaagatt
    11221 ggtgttcggg tagcggagga agaagccgaa tcttaagttt aatgtgtttc gtaacgctgg
    11281 atacgacgct ggtcgcatcc agcgataaca agttacaccg tcacaaccgg gacgcgtaac
    11341 gccagcgcgc acatcaactc atagcctacc gttccggcag cggcggcgac atcatcaatt
    11401 ttgatctcct tgccccacag ctcaactggc gtaccaatac ccgcttgcgg gcaaggcgtt
    11461 aaatcgaccg ccagcatatc catcgagact gtccccaccg tcatggtgcg aacgccgtcc
    11521 actaaaacag gggtaccggt aggcgcgtgg cgcggataac cgtcggcgta tcctgcggcg
    11581 acaatgccga ttcgctgttc atcgcgcgca gtatagcgac cgccgtagcc cacacgctcg
    11641 cccgctttta gcgtctggac accaataatc tcactgctta gcgtcatcac cggacgtaat
    11701 ccggtattgg cgatatcacg ccactgaccg gacggcgaag caccatacaa aataatgcca
    11761 ggccgaaccc agtcaaaatg cgcttcctgg tgccacagag tcgccgccga attagacaac
    11821 gaacgccgac actccagccc ttccgccgcc tgctcaatac gcgccatcgc gctggaaatt
    11881 ccatcaggat gttccgcttc ggcaaaatgc gacatcaggg tcatttcgcc aacattcgcc
    11941 atcgcccgca actgctgcca gacggtaagc acgcgatcgg gctggaagcc caaccgattc
    12001 atcccactgt tcactttaag ataaatatcc aacggtgctt ttagccgcgc attttgcagt
    12061 gctttgagct gccagttgct gtgtacgcag gtggtcaggc ggtgctggtc ataaatctcc
    12121 agatcctgag catggaaaaa tccttccagc atcaggatcg gtcctttcca gccgcgctca
    12181 cgtaacgtta ttgcctcttc caggttaagc aatgcaaagc catcggtagc cccgagcgcg
    12241 ctccagatac gctcaatacc gtgcccgtaa gcgttcgctt ttaccaccga ccagacgcgc
    12301 gcgtgcggcg cggcctggcg gacaatggac agattctgtt ttaatgcctg cagatcgagg
    12361 ctggcctgta tcggacgggt catctcgttt ccttagctgt gtgcgccatg taaatggccc
    12421 gggcgtgatg gcgtaaatcc acggctgtag cgcgctacgc ttagatcttc atacgggatc
    12481 gctggcgtgc gccccgagag cagatcgctt aacaactgac cggaaccgca agccatcgtc
    12541 cagccgagcg tgccgtgacc ggtattcaac cacagatttt taaagcgtgt acgcccgaca
    12601 accggcgtgc cgtctggcgt catcgggcgc aggccagtcc agaaagccgc ctgatcaaca
    12661 tgaccgccgc gtggatagag atcgcgaacc accatctcca gcgtttcacg acgcggttgc
    12721 aacagctcgg tgttaaaacc aacaatttcc gccattccgc cgacacgaat gcggttatcg
    12781 aaacgggtaa tggcgatttt gtaggtttca tcaagaatag tggataccgg cgcaccatct
    12841 tcctgcgcaa ttggaatggt cagcgagtag cctttcagcg gatacaccgg aatatcaaca
    12901 atgcctttga gcatcgccgt cgagtaagaa ccaaacgcca tcacatacgc atcggcctta
    12961 atcacttcat caccacactt cacgccgtag atttgctcgc cgtcgcaaag cagttggtca
    13021 acgggcgtat taaagcggaa tttaaccccc gcctgctccg ccatccgcgc cagattctgg
    13081 gtaaacagct ggcagtctcc ggtttcatca ttgggcagtt gcaggccgcc cgtcagtttg
    13141 tgcgctactt ctgccaacgc gggctccact tccgccaggc ggctggattc cagcagctga
    13201 tacggtacgc cagcatcttc cagcacggcg atatcgcggg tcgcgttttc atactgttgt
    13261 tcggtacgga acagttgcag cgtcccaccc tgacgccctt catactgaat attggtttcg
    13321 gcgcgcaatg ctttcaggca atcacggctg tattccgcca gacgtaccat ccgccctttg
    13381 ttttccatgt agtggctggt gtcgcagtta cgtaacattt gccacatcca tttcaactgg
    13441 aactgcgtac cgtcgagacg aaccgccagc ggcgcatggc gctggaacat ccatttaatc
    13501 gcttttaaag gcacacctgg tgccgcccac ggtgcagcat atccggggga gatttgcccg
    13561 gcattcgcgg cactggtttc cagggcggcc cccggctccc gatcaatgac ggtgacctca
    13621 tgtcctgcct gatttaagta ccaggcgctg gcaacgccta ccacaccact tcccagtatg
    13681 acaactcgca tagccactcc gataacggta gatacagtaa aagaataata atc
//
LOCUS       DAHHTG010000112        13604 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.113, whole genome shotgun sequence.
ACCESSION   DAHHTG010000112 DAHHTG010000000
VERSION     DAHHTG010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13604)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13604)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13604
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.113"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            <1..1321
                     /gene="chbC"
                     /locus_tag="NBI74_003463"
     CDS             <1..1321
                     /gene="chbC"
                     /locus_tag="NBI74_003463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIC"
                     /protein_id="HCD1933745.1"
                     /translation="PFAVKIGKQPHVNAIKNGFIRLMPLTLAGAMFVLINNVFLSFGE
                     GSFFYSLGIRLDASTIETLNGLKGIGGNVYNGTLGIMSLMAPFFIGMALAEERKVDAL
                     AAGLLSVAAFMTVTPYSVGEAYAVGANWLGGANIISGIIIGLVVAEMFTFIVHRNWVI
                     KLPDSVPTSVSRSFSALIPGFIILSVMGIIAWALNTWGTNFHQIIMDTISTPLASLGS
                     VVGWAYVIFVPLLWFFGIHGALALTALDNGIMTPWALENIATYQQYGSVEAALAAGKT
                     FHIWAKPMLDSFIFLGGSGATLGLILAIFIASRRADYRQVAKLALPSGIFQINEPILF
                     GLPIIMNPVMFIPFVLVQPILAAITLAAYYMGIIPPVTNIAPWTMPTGLGAFFNTNGS
                     VAALLVALFNLGIATLIYLPFVVVANKAQNAIDKEESEEDIANALKF"
     gene            1372..1722
                     /gene="chbA"
                     /locus_tag="NBI74_003464"
     CDS             1372..1722
                     /gene="chbA"
                     /locus_tag="NBI74_003464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIA"
                     /protein_id="HCD1933746.1"
                     /translation="MMDLDNIPDTQTEAEELEEVVMGLIINSGQARSLAYAALKQAKQ
                     GDFAAAKAMMDQSRMALNEAHLVQTKLIEGDAGEGKMKVSLVLVHAQDHLMTSMLARE
                     LITELIELHEKLKA"
     gene            1730..2572
                     /gene="chbR"
                     /locus_tag="NBI74_003465"
     CDS             1730..2572
                     /gene="chbR"
                     /locus_tag="NBI74_003465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ChbR"
                     /protein_id="HCD1933747.1"
                     /translation="MMQPVINAPEIATAREQQLFNGKNFHVFIYNKTESISGLHQHDY
                     YEFTLVLTGRYFQEINGKRVLLERGDFVFIPLGSHHQSFYEFGATRILNVGISKRFFE
                     QHYLPLLPYCFVASQVYRTNNAFLTYVETVISSLNFRETGLEEFVEMVTFYVINRLRH
                     YREEQVIDDIPQWLKSTVEKMHDKEQFSESALENMVTLSAKSQEYLTRATQRYYGKTP
                     MQIINEIRINFAKKQLEMTNYSVTDIAFEAGYSSPSLFIKTFKKLTSFTPKSYRKKLT
                     EFNQ"
     gene            2675..4027
                     /gene="celF"
                     /locus_tag="NBI74_003466"
                     /gene_synonym="chbF"
     CDS             2675..4027
                     /gene="celF"
                     /locus_tag="NBI74_003466"
                     /gene_synonym="chbF"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HCD1933748.1"
                     /translation="MSQKLKVVTIGGGSSYTPELLEGFIKRYHELPVSELWLVDVEGG
                     KAKLDIIFDLCQRMIDNAGVPMKLYKTLDRREALKDADFVTTQLRVGQLPARELDERI
                     PLSHGYLGQETNGAGGLFKGLRTIPVIFDIVKDVEELCPNAWVINFTNPAGMVTEAVY
                     RHTGFKRFIGVCNIPIGMKMFIRDVLMLKDSDDLSIDLFGLNHMVFIKDVLVNGKSRF
                     AELLDGVASGQLKASGVKNIFDLPFSEGLIRSLNLLPCSYLLYYFKQKEMLAIEMGEY
                     YKGGARAQVVQKVEKQLFELYKNPELKVKPKELEQRGGAYYSDAACEVINAIYNDKQA
                     EHYVNIPHHGHIDNIPADWAVEMTCTLGRDGATPHPRITHFDDKVMGLIHTIKGFEIA
                     ASNAALSGEFNDVLLALNLSPLVHSDRDAELLAREMILAHEKWLPNFADCIAELKKAH
                     "
     gene            4040..4798
                     /gene="chbG"
                     /locus_tag="NBI74_003467"
     CDS             4040..4798
                     /gene="chbG"
                     /locus_tag="NBI74_003467"
                     /EC_number="3.5.1.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitin disaccharide deacetylase"
                     /protein_id="HCD1933749.1"
                     /translation="MERLLIVNADDFGLSKGQNYGIIEACRNGIVTSTTALVNGQAID
                     HAVQLSRDEPSLAIGMHFVLTMGKPLTAMPGLTRDGVLGKWIWQLAEEDALPLEEITQ
                     ELASQYLRFIELFGRKPTHLDSHHHVHMFPQIFPIVAKFAAEEGIALRIDRQSLSKDG
                     DLPANLRSSQGFSSAFYGEEISETLFLQVLDDSSHRGERSLEVMCHPAFIDNTIRQSA
                     YCFPRLTELDVLTSASLKYAIAERGYRLGSYHDV"
     gene            complement(4845..7106)
                     /gene="katE"
                     /locus_tag="NBI74_003468"
     CDS             complement(4845..7106)
                     /gene="katE"
                     /locus_tag="NBI74_003468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase HPII"
                     /protein_id="HCD1933750.1"
                     /translation="MSQHNEKNPHQHQSPLHDSSEAKPGMDSLAPEDGSHRPAAEPTP
                     PGAQPTAPGSLKAPDTRNEKLNSLEDVRKGSENYALTTNQGVRIADDQNSLRAGSRGP
                     TLLEDFILREKITHFDHERIPERIVHARGSAAHGYFQPYKSLSDITKADFLSDTNKIT
                     PVFVRFSTVQGGAGSADTVRDIRGFATKFYTEEGIFDLVGNNTPIFFIQDAHKFPDFV
                     HAVKPEPHWAVPQGQSAHDTFWDYVSLQPETLHNVMWAMSDRGIPRSYRTMEGFGIHT
                     FRLINAEGKATFVRFHWKPLAGKASLVWDEAQKLTGRDPDFHRRELWEAIEAGDFPEY
                     ELGFQLIPEEDEFKFDFDLLDPTKLIPEELVPVQRVGKMVLNRNPDNFFAENEQVAFH
                     PGHIVPGLDFTNDPLLQGRLFSYTDTQISRLGGPNFHEIPINRPTCPYHNFQRDGMHR
                     MGIDTNPANYEPNSINDNWPRETPPGPKRGGFESYQERVEGNKVRERSPSFGEYYSHP
                     RLFWLSQTPFEHRHIVDGFSFELSKVVRPYIRERVVDQLAHIDLTLAQAVAKNLGIEL
                     TDDQLNITPPPNVNGLKKDPSLSLYAIPDGDVKGRVVAILLNDEVRSADLLAILKALK
                     AKGVHAKLLYSRMGEVTADDGTVLPIAATFAGAPSLTVDAVIVPCGNIADIANNGDAN
                     YYLMEAYKHLKPIALAGDARKFKATIKVADQGEEGIAEADRADGSFMDELLTLMTAHR
                     VWSRIPKIDKIPA"
     gene            7289..7552
                     /gene="cedA"
                     /locus_tag="NBI74_003469"
     CDS             7289..7552
                     /gene="cedA"
                     /locus_tag="NBI74_003469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division activator CedA"
                     /protein_id="HCD1933751.1"
                     /translation="MRLVKPVMKKPLRQQNRQIISYVPRTEPAPPEHAIKMDSFRDVW
                     MLRGKYVAFVLMGESFLRSPAFTVPESAQRWANQIRQENEVEE"
     gene            complement(7599..8990)
                     /gene="tcyP"
                     /locus_tag="NBI74_003470"
     CDS             complement(7599..8990)
                     /gene="tcyP"
                     /locus_tag="NBI74_003470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine/sulfocysteine:cation symporter"
                     /protein_id="HCD1933752.1"
                     /translation="MNFPLIANIVVFVVLLFALAQTRHKQWSLAKKVLVGLVMGVVFG
                     LALHTIYGSDSQVLKDSVQWFNIVGNGYVQLLQMIVMPLVFASILSAVARLHNASQLG
                     KISFLTIGTLLFTTLIAALVGVLVTNLFGLTAEGLVQGGAETARLNAIESNYVGKVSD
                     LSVPQLVLSFIPKNPFADLTGANPTSIISVVIFAAFLGVAALKLLKDDAPKGERVLTA
                     IDTLQSWVMKLVRLVMQLTPYGVLALMTKVVAGSNLQDIIKLGSFVVASYLGLLIMFA
                     VHGLLLGINGVSPLKYFRKVWPVLTFAFTSRSSAASIPLNVEAQTRRLGVPESIASFA
                     ASFGATIGQNGCAGLYPAMLAVMVAPTVGINPLDPMWIATLVGIVTVSSAGVAGVGGG
                     ATFAALIVLPAMGLPVTLVALLISVEPLIDMGRTALNVSGSMTAGTLTSQWLKQTDKA
                     ILDSEDDAELAHR"
     gene            complement(9123..9713)
                     /locus_tag="NBI74_003471"
     CDS             complement(9123..9713)
                     /locus_tag="NBI74_003471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310461.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HCD1933753.1"
                     /translation="MTAEGHLLFSIACAVFAKNAELTPVLAQGDWWHIVPSAILTCLL
                     PDIDHPKSFLGQRLKWISKPIARAFGHRGFTHSLLAVFALLATFYLKVPESWFIPADA
                     LQGMVLGYLSHILADMLTPAGVPLLWPCRWRFRLPILVPQKGNQLERFICMALFVWSV
                     WMPHSLPENSAVRWSSQMINTLQIQFHRLIKHQVEY"
     gene            complement(9877..10545)
                     /gene="hxpB"
                     /locus_tag="NBI74_003472"
     CDS             complement(9877..10545)
                     /gene="hxpB"
                     /locus_tag="NBI74_003472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpB"
                     /protein_id="HCD1933754.1"
                     /translation="MSTPRQILAAIFDMDGLLIDSEPLWDRAELDVMASLGVDISRRN
                     ELPDTLGLRIDMVVDLWYARQPWNGPSRQEVVEQVIARAISLVEETRPLLPGVREAVA
                     LCKEQGLLVGLASASPLHMLEKVLTMFDLRDSFDALASAEKLPYSKPHPQVYLDCAAK
                     LGVDPLTCVALEDSVNGMIASKAARMRSIVVPAPEAQNDPRFVLANVKLSSLTELTAK
                     DLLG"
     gene            10692..11228
                     /locus_tag="NBI74_003473"
     CDS             10692..11228
                     /locus_tag="NBI74_003473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YniB family protein"
                     /protein_id="HCD1933755.1"
                     /translation="MTYQQAGRIAVLKRILGWVIFIPALISTLISLLKFMNTRQENQE
                     GINAVMLDFTHVMIDMMQANTPFLNLFWYNSPTPNFNGGVNVMFWVIFILIFVGLALQ
                     DSGARMSRQARFLREGVEDQLILEKAKGEEGLTREQIESRIVVPHHTIFLQFFSLYIL
                     PVICIAAGYVFFSLLGFI"
     gene            complement(11269..12129)
                     /locus_tag="NBI74_003474"
     CDS             complement(11269..12129)
                     /locus_tag="NBI74_003474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HCD1933756.1"
                     /translation="MWQAISRLLSEQLGEGEIELRNELPGGEVHAAWHLRYAGRDFFV
                     KCDERELLPGFTAEADQLELLSRSKTVTVPKVWAVGADRDYSFLVMDYLPPRPLDAHS
                     AFILGQQIARLHQWSDQPQFGLDFDNALSTTPQPNTWQRRWSTFFAEQRIGWQLELAA
                     EKGIAFGNIDAIVEHIQQRLASHQPQPSLLHGDLWSGNCALGPDGPYIFDPACYWGDR
                     ECDLAMLPLHTEQPPQIYDGYQSVSPLPADFLERQPVYQLYTLLNRARLFGGQHLVIA
                     QQSLDRLLAA"
     gene            complement(12235..12525)
                     /gene="ghoS"
                     /locus_tag="NBI74_003475"
     CDS             complement(12235..12525)
                     /gene="ghoS"
                     /locus_tag="NBI74_003475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HCD1933757.1"
                     /translation="MASGDLVRYVITVMLHEDTLTEINELNNYLTRDGFLLTMTDDDG
                     NIHELGTNTFGLISTQSEEEIRELVSGLTHSATGKDPEITITTWEEWNSNRK"
     gene            complement(12626..13555)
                     /gene="pfkB"
                     /locus_tag="NBI74_003476"
     CDS             complement(12626..13555)
                     /gene="pfkB"
                     /locus_tag="NBI74_003476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416237.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase II"
                     /protein_id="HCD1933758.1"
                     /translation="MVRIYTLTLAPSLDSATITPQIYPEGKLRCTAPVFEPGGGGINV
                     ARAIAHLGGTATAIFPAGGATGEHLVSLLADENVPVATVEAKDWTRQNLHVHVEASGE
                     QYRFVMPGAALNEDEFRQLEEQVLEIESGAILVISGSLPPGVKLEKLTQLISAAQKQG
                     IRCIVDSSGEALSAALAIGNIELVKPNHKELSALVNRELTQPDDVRKAAQEIVNSGKA
                     KRVVVSLGPQGALGVDSENCIQVVPPPVKSQSTVGAGDSMVGAMTLKLAENASLEEMV
                     RFGVAAGSAATLNQGTRLCSHDDTQKIYAYLSR"
BASE COUNT         3414 a         3216 c         3482 g         3492 t
ORIGIN      
        1 cccttttgca gttaaaatag gaaagcagcc acacgttaat gcaatcaaaa atggctttat
       61 tcgcttaatg ccgttaaccc ttgcgggggc catgtttgta ttaattaaca acgtttttct
      121 aagctttggg gaggggtcgt ttttttattc cttaggtatt cgcctggatg cctcaaccat
      181 tgaaacactt aatggtctga aaggtattgg cggcaacgta tataacggaa cattagggat
      241 aatgtcttta atggcaccgt tctttattgg catggcgctg gcagaagagc gtaaagtcga
      301 tgcgctggcg gccgggttgt tatccgttgc agcatttatg accgtcaccc catatagtgt
      361 cggtgaggcc tatgcggttg gcgcaaactg gttaggtggg gcgaatatca tctccgggat
      421 tattattggc ctggtggtgg cagaaatgtt tacctttatt gttcatcgta actgggtcat
      481 taagttacct gatagcgttc cgacatcggt ttcgcgctct ttctcagcat taattcctgg
      541 ttttattatt ctttccgtga tggggattat tgcctgggcg ttgaatacct ggggcaccaa
      601 cttccatcag atcattatgg atacgatctc aaccccactg gcatcgttgg gtagcgtggt
      661 aggctgggcc tatgtgatct ttgttccact gctctggttc ttcggtattc atggcgcgct
      721 ggcgctgacc gcactggaca acggcattat gacgccttgg gcgctggaaa atatcgcgac
      781 ctatcagcaa tatggttccg tcgaagcggc gctggcagcc ggtaagacct tccatatctg
      841 ggccaagccg atgctggact cctttatttt ccttggtggc agtggtgcga ctttaggcct
      901 gatcctggct atctttatcg cctctcgacg tgctgattat cgtcaggtgg caaaactggc
      961 gctgccgtct ggcatcttcc agattaacga accgattctg tttggtctgc caattatcat
     1021 gaacccggtg atgtttatcc cgtttgtact ggtacaaccg attctggcgg caatcaccct
     1081 ggcagcgtac tacatgggca ttattccacc ggtgaccaat attgcaccgt ggaccatgcc
     1141 aaccggtctg ggagccttct ttaacaccaa cggtagcgtc gccgcattgc tggtcgcact
     1201 cttcaacctt ggtatcgcaa cgttaattta tctgcccttt gttgtggtcg ctaacaaagc
     1261 acaaaacgcg attgataaag aagagagcga agaagatatc gctaacgccc tgaaatttta
     1321 atcgatgccg gtacgggtaa tcgtgccggt aagaatagag aggaacgatg tatgatggat
     1381 ctcgataata ttcccgatac gcaaacggaa gctgaagagc tggaagaagt ggtgatgggg
     1441 cttatcatca actccggaca agcgcgtagc ctggcgtatg cggcactgaa acaggcgaag
     1501 cagggcgatt ttgccgcagc aaaagcgatg atggatcagt cacggatggc attgaatgaa
     1561 gcgcatctgg tacagacgaa actgattgaa ggcgatgctg gcgaaggtaa gatgaaagtg
     1621 agtctggtgc tggtccacgc tcaggatcat ttaatgacgt ccatgcttgc gcgtgaactg
     1681 attactgaat taattgagct tcatgaaaaa ctgaaggcat aaggagtcga tgatgcagcc
     1741 agtgattaac gcgccggaaa ttgccactgc ccgagaacag cagttgttta atggcaaaaa
     1801 cttccatgtg tttatctata ataaaactga gagtatcagc ggactgcatc agcacgacta
     1861 ttatgaattt actctggtat taaccgggcg ttatttccag gagattaacg gtaagcgcgt
     1921 gttactggaa cggggcgatt ttgtttttat tccgttaggt tcgcaccatc aaagttttta
     1981 tgagtttggt gccacgcgta tattgaacgt tgggatcagt aaacgctttt ttgagcagca
     2041 ttacctgccg ttgttgcctt attgctttgt agcttcgcag gtataccgga ccaataacgc
     2101 ttttctcacc tatgtggaaa cagtgatttc ttcattgaat ttccgcgaaa cagggctgga
     2161 agagtttgtt gagatggtta ctttttatgt cattaaccgt ttacgtcatt accgcgaaga
     2221 acaggtgatt gatgatatac cgcagtggct gaaaagtacg gtagaaaaaa tgcatgataa
     2281 agagcagttt agtgaatcgg cgctggagaa tatggtgacg ttgtcagcca aatcacagga
     2341 atatttgacg cgagcgactc aacgatatta tggtaaaacg ccaatgcaga ttattaatga
     2401 aatccgtatt aattttgcca aaaaacaact ggaaatgacc aactattcag tgacggatat
     2461 tgcgtttgag gccggttata gtagcccgag cttgtttatt aaaacgttta agaaattaac
     2521 gtcctttacg cctaagagct atcgtaagaa attgactgaa tttaatcagt aagtcgttat
     2581 acctgacaat tcacatatct gtcctgttgc tggattattt atgtccgggg gcagatatgc
     2641 cagatatcag tattctgtac tgaagggaga aattatgagc cagaaattaa aagtcgtcac
     2701 tattggtggc gggagcagct ataccccgga gttactggaa ggatttatta agcgttatca
     2761 cgaattgccg gtcagcgaat tatggctggt ggatgtcgaa ggtggtaaag cgaaactgga
     2821 tattattttt gatctctgcc aacggatgat tgataacgct ggcgtcccga tgaagcttta
     2881 taaaacgctg gatcgccgcg aagcattgaa agatgctgat ttcgttacta cccaactgcg
     2941 cgttggccaa ttaccggcgc gggaactgga tgaacgtatt ccattaagtc atggttatct
     3001 tggtcaggaa actaacggcg cgggcggtct gtttaaaggt ctgcgtacca ttccggtgat
     3061 ttttgacatc gtaaaagatg tcgaagaact atgtccgaat gcatgggtga ttaacttcac
     3121 taacccggcg ggaatggtca ctgaagccgt ttatcgtcat accggattta aacgctttat
     3181 cggcgtgtgt aatattccga tcggcatgaa gatgtttatt cgcgatgttc tgatgctgaa
     3241 agacagcgat gatttatcta tcgatctgtt cggcctcaac catatggtgt tcattaagga
     3301 tgtgctggta aatggcaaat cacgctttgc cgaattgctt gatggtgtgg catccgggca
     3361 gttaaaagca tctggcgtta aaaatatttt cgatctgcca tttagcgaag gcttaattcg
     3421 ttctctgaat ctgttgccgt gttcttattt gctttattac ttcaagcaaa aagagatgct
     3481 ggctattgaa atgggcgaat actacaaagg cggcgcacga gcgcaggtcg tacagaaagt
     3541 cgagaaacaa ctttttgagc tatataaaaa cccggagttg aaagttaagc cgaaagaact
     3601 ggaacagcgc ggtggggctt attattctga tgctgcgtgc gaagtgatca acgctatcta
     3661 taacgacaag caagcagaac attacgttaa tatcccgcat catgggcata ttgataatat
     3721 tccggcagac tgggcggtag aaatgacctg tacgctgggg cgcgatggcg cgacgccaca
     3781 tccgcgcatt acgcatttcg atgataaagt gatggggctg attcacacca tcaaaggctt
     3841 cgagattgct gccagcaacg ccgcacttag tggagaattt aacgatgtct tactggcgct
     3901 aaaccttagt ccgttggtgc attccgatcg cgatgctgag ctgctggcac gcgagatgat
     3961 tctggcgcac gagaaatggc tgccaaattt tgccgactgc atcgcagagc ttaaaaaagc
     4021 acattaaccg aggctgatta tggaacgctt actgattgtt aatgctgatg attttggctt
     4081 aagcaaaggt cagaactacg gcattatcga ggcctgtcgc aatgggattg tcacgtcgac
     4141 gacggcgctg gtgaatgggc aggctattga ccatgcggtg cagttgagcc gtgatgaacc
     4201 gagtctggcc atagggatgc actttgtcct tactatgggc aagcctctga cagctatgcc
     4261 ggggttaacc cgcgatggtg tgctgggaaa atggatctgg cagttggcag aagaagatgc
     4321 tttaccgctg gaagaaatta ctcaggagct cgccagtcag tatttgcgtt tcattgagct
     4381 atttggacgc aaacctacgc atcttgatag ccatcatcat gtgcatatgt tcccgcagat
     4441 tttcccgatt gtggcaaagt ttgcggctga agaggggatt gcgttgcgca tcgaccgtca
     4501 gtcgctttct aaggatggtg atttaccggc taatctgcgc agttcgcagg gattcagtag
     4561 cgcgttttat ggtgaagaga ttagcgagac actgttcctg caagtgcttg atgactccag
     4621 tcatcgcggc gagcgctcgc tggaagtgat gtgccacccg gcgtttatcg ataatacgat
     4681 tcgccagagt gcttattgct tccctcggtt aacggaactg gatgtgctga cttcagcgtc
     4741 gttgaaatat gcgattgctg agcgtggtta tcggttgggt agttatcatg atgtgtaagt
     4801 aaggtggtaa ggtcactccc cagggggcgt gaccttatcc atatttacgc cgggattttg
     4861 tcaatcttag gaatgcgtga ccacacgcgg tgtgctgtca tcagcgttaa cagttcatcc
     4921 ataaaactac catcggcgcg gtcagcttcc gcaatccctt cttcaccctg gtcagcgacc
     4981 ttgattgttg ctttaaactt gcgcgcgtct cctgccagcg caatcggttt aaggtgtttg
     5041 taggcttcca tcagatagta gttggcatcg ccgttgttag cgatatccgc aatattgccg
     5101 caagggacaa tgactgcatc gaccgtcagc gaaggcgcac cggcaaaggt agcagctata
     5161 ggcaacaccg taccgtcatc cgcagtcact tcccccattc gcgagtagag cagtttggca
     5221 tgaacgcctt tggccttcag cgccttgaga atggccagaa ggtctgccga tctcacttca
     5281 tcattaagca aaattgctac cacgcgacct ttcacatcac cgtcaggaat ggcgtacaga
     5341 cttaaggatg gatccttttt cagaccgttg acgttcggag gcggggtgat attcaactgg
     5401 tcgtcagtca gttcgatacc gagatttttc gccaccgcct gggccagagt gagatcaata
     5461 tgcgccagct ggtcaacaac gcgctcacga atatacggcc gaactacttt gcttaactca
     5521 aaactgaaac catcgacaat atggcgatgc tcgaatggcg tctgacttag ccagaacaga
     5581 cgcggatggg aataatattc gccaaacgat ggactgcgct cgcgaacttt attgccttcc
     5641 acgcgttcct ggtatgattc aaaaccgcca cgttttggcc ccggcggtgt ttcgcgcggc
     5701 cagttatcgt tgatcgagtt cggttcgtaa ttcgccgggt tagtgtcgat ccccatacga
     5761 tgcatgccgt cacgctggaa attatggtaa gggcaggtcg ggcggttaat cggaatttca
     5821 tggaaattcg gcccaccaag acgactgatt tgtgtatcgg tataagagaa caaacgcccc
     5881 tgcaataacg ggtcgttggt gaaatccaga ccggggacaa tatgccctgg atggaaagcc
     5941 acctgttcgt tttcagcaaa gaagttatcc ggattacgat tgagcaccat tttgccgaca
     6001 cgctgaacgg gcaccaattc ttccgggata agtttggttg gatcgagaag atcgaagtcg
     6061 aacttgaatt cgtcttcttc aggaatcaac tggaagccca gttcgtattc aggaaaatcg
     6121 cctgcttcaa tcgcttccca caactcgcgg cggtggaagt ccgggtcacg tccggttagt
     6181 ttttgtgctt catcccaaac gagtgaggct ttacctgcca gtggtttcca gtggaaacgt
     6241 acgaacgttg ccttcccttc ggcattaatc aggcggaagg tatgaatacc gaagccttcc
     6301 atagtgcggt aactgcgcgg gataccgcga tccgacattg cccacatcac gttgtgcaga
     6361 gtttcaggtt gcagagaaac ataatcccag aaagtatcgt gggcactttg cccttgtgga
     6421 actgcccagt gcggttctgg ttttaccgca tgaacaaaat cggggaattt atgcgcatcc
     6481 tggataaaga agattggcgt gttattgcca acgaggtcaa aaataccctc ttcagtatag
     6541 aacttggtgg caaagccacg gatatcacgc acggtatcgg cagagccagc gccgccctga
     6601 acggtagaga acctaacaaa tactggggtg attttgttcg tatctgagag gaaatccgct
     6661 ttggtgatat cgcttaagct tttatatggc tggaaataac cgtgagctgc tgatccgcgt
     6721 gcatgaacaa tacgttccgg gatgcgctca tggtcaaagt gagtgatttt ctcgcgcaga
     6781 ataaaatctt ccagcagagt tggaccacgg ctaccggcac gcagtgagtt ttgatcgtcg
     6841 gcgatgcgca cgccctgatt agtggtcagc gcataatttt cactgccttt gcgtacgtct
     6901 tccagagaat taagtttttc gttacgcgta tcaggggctt tcaggctccc tggggcggta
     6961 ggttgtgcac caggcggtgt tggttcagcc gctggacgat gagatccgtc ttcaggtgcc
     7021 agtgagtcca tccccggttt cgcttcgctg gaatcgtgta gtggtgactg gtgctgatgt
     7081 gggttctttt cgttatgttg cgacattgaa ctcgtctcct taaattatta ctgaaagggc
     7141 cgctgttttg gtcaaaaaac cctctaacta tagaccacca gccagatccc gcttcggaaa
     7201 ctattctgga aacggtgtta tgaaatacac gcaaagcggg acgtacaggg gctaaatcgg
     7261 ctaaactaca gtttttctga tttttaatat gcgtttagtg aagccagtaa tgaagaaacc
     7321 gctccgtcag caaaaccgcc agattattag ctatgtccca cgcacggaac ccgcgccgcc
     7381 agaacatgcg ataaagatgg attcgtttcg agatgtctgg atgctgcgtg gcaaatatgt
     7441 tgcgtttgta ctgatggggg agtcatttct gcgctcaccg gcgtttactg tgcctgaatc
     7501 cgcccagcgt tgggcaaatc agatccgcca ggaaaatgag gtcgaagaat aaaaaaacac
     7561 ctgtcgggat gacaggcgtt taacgtaaca aatcaggatt aacggtgtgc cagttcggcg
     7621 tcgtcttcgc tatccagaat ggctttatcg gtttgcttca gccactggct ggtcagcgtg
     7681 ccagcggtca tcgagccact aacgtttagc gccgtacggc ccatgtcgat aagcggttca
     7741 acggagatta acaatgccac cagtgttacc ggcaggccca tcgcaggcag cacaatcagt
     7801 gcggcgaaag ttgcaccacc accgacaccg gcaacgcctg cggaactaac ggtgacaata
     7861 ccgaccagcg tcgcaatcca catcgggtcc agcgggttaa tgcccaccgt aggcgcaacc
     7921 atcaccgcca gcattgccgg atacaaaccg gcacaaccgt tctgaccaat ggtcgcaccg
     7981 aaagaggcgg cgaaactggc gatggattca ggaacaccca gacgacgcgt ttgtgcttcc
     8041 acattcagtg ggatagacgc agcactggaa cggctggtaa aggcaaacgt cagtactggc
     8101 cataccttac ggaagtactt cagcgggctc acgccattaa tgcccagcag aaggccatgc
     8161 actgcaaaca taatcagcag accgaggtag gacgcgacaa cgaaacttcc cagtttgatg
     8221 atgtcttgca ggttagaacc tgcaaccact ttggtcatca gcgccagaac accgtaaggg
     8281 gtcaactgca tgaccaggcg aaccagtttc atcacccagc tttgcagggt atcgatagcg
     8341 gttaagacgc gttcaccttt cggcgcatca tccttcagca gtttcagcgc agctacgccg
     8401 aggaatgcgg caaaaattac cacgctgata attgacgtcg gattggctcc ggtcagatcg
     8461 gcaaacgggt ttttcgggat aaaggacaag accagctgcg gaacgctcag gtcagagact
     8521 ttaccaacat agttactttc gatggcgttc agacgtgcag tttctgcacc accctgaacc
     8581 agaccttcag ccgtcaaacc aaacaggttg gtgaccagca caccaaccag cgctgcaatc
     8641 agcgtggtaa acaaaagcgt accgatggtc agaaaactga ttttgcctaa ctgagatgcg
     8701 ttatgcagac gggcaaccgc gctcagaata gaggcgaaga ctagcggcat aacgatcatt
     8761 tgcagcagtt gaacatagcc gttaccaaca atgttaaacc actgtacaga atctttaagt
     8821 acctggctgt cagaaccata aatggtatgc agggcaaggc caaaaaccac acccatcaca
     8881 agacccacca gcactttttt cgccagactc cactgtttgt ggcgggtttg agccagcgca
     8941 aacagcagta caacgaacac cacgatgttc gcaattaatg gaaagttcat ccccgttctc
     9001 ctgatcttat tatttctatc actgcgtagc agtcatagtg ttggcgcaag attaacagaa
     9061 ctgtgagctg gctcttatat tcaaatggaa ttgcttattc cagaaaggcg atttttgcct
     9121 ttttagtatt caacctgatg cttaataagc cgatgaaact ggatctgtaa ggtattgatc
     9181 atttgcgatg accaacgaac agcgctgttt tcgggtaatg aatggggcat ccataccgac
     9241 cagacaaata atgccatgca gataaaacgt tccagttggt tgcccttttg gggaaccagg
     9301 ataggcaagc ggaaacgcca gcggcatggc cagagcaggg gaacaccggc gggtgtcagc
     9361 atatcggcaa gtatgtggct caaataaccc agcaccatac cctgcaacgc atcagccgga
     9421 atgaaccagc tttcaggaac cttaaggtaa aaggttgcca gcagcgcaaa taccgccagc
     9481 aggctgtggg taaaaccacg atgcccaaaa gcgcgggcga tcggttttga tatccacttt
     9541 aatcgctgcc caagaaacga ctttgggtga tcgatgtccg gtaacaaaca cgtcaggatt
     9601 gcggaaggga caatatgcca ccagtccccc tgtgccagca cgggcgtcag ctcggcattt
     9661 ttggcaaata ccgcacaagc aatagaaaag agaaggtgac cttccgccgt catgataaaa
     9721 ctcactaaac tgtcgatacg tacagtatag ggtttttata cagtgcacgg aagtggtgac
     9781 ggtgtaactc tttctcttaa attggcgatt aatccgtcat ttgaatgcaa attcagataa
     9841 agaaccaggg gcagtgtgat cactgccccc tggtcattaa ccgagaaggt cttttgcggt
     9901 gagttctgtc agcgatgaga gtttgacgtt tgctaataca aaacgtggat cattttgcgc
     9961 ttctggcgca ggaacgacga tggaacgcat gcgggctgct ttagaggcga tcatgccatt
    10021 taccgaatct tccagtgcca cgcaggtcag agggtcaacc cccagttttg ctgcgcagtc
    10081 aagatatact tgcggatgcg gcttgctgta aggcagtttt tcagccgagg cgagggcatc
    10141 gaaactgtcg cgtaaatcaa acatggtcaa tactttttcc agcatatgca gcggtgacgc
    10201 ggaggccagc cccaccaata aaccttgttc tttgcataac gcaacggctt cacgcacacc
    10261 tggcaataat ggacgtgtct cttcaaccag tgaaatggca cgggcaataa cttgttctac
    10321 tacttcctga cggcttggcc cattccatgg ttgccgggcg taccaaagat cgaccaccat
    10381 atcgatgcgt aaacctaagg tgtccggcag ctcgttacga cgggagatat ccacccccag
    10441 gcttgccatc acatccagtt cggctcgatc ccataaaggt tctgagtcga taagtaatcc
    10501 atccatatca aaaattgcag caagaatctg acgcggggtt gacattacaa cctctccttt
    10561 agagcattat atgtaaggta ttaagccgtg cttatgatta agcgcctatc atatcgcctt
    10621 taaagaatgg gcgaaaacag atatctacag gtaaactaag tgaaacgacg cgtcttaatc
    10681 aaggagaact catgacgtat caacaagctg gacgcattgc tgttttgaaa aggattttgg
    10741 gttgggtgat ttttatacct gcattgatct ctacattgat ttctttgctg aaatttatga
    10801 atacccggca ggaaaaccag gaaggcatta atgcggtcat gctcgacttt actcatgtca
    10861 tgatcgatat gatgcaggcg aatacgcctt ttttgaatct gttctggtac aactctccga
    10921 cgcccaattt taatggtggc gtgaacgtta tgttctgggt gattttcatc ctgatttttg
    10981 tcggactggc gttgcaggat tccggagccc ggatgagtcg ccaggcgcgc ttcttgcggg
    11041 aaggtgttga agatcaactc attctggaaa aagccaaagg ggaagagggg ctaacgcgtg
    11101 aacaaattga gtcacgtatt gttgttccgc accatactat tttcctgcag tttttctcgc
    11161 tgtatatcct gccggttatt tgtattgctg caggttatgt gttcttttct ctgcttgggt
    11221 ttatttaatc aaaaggccgc acggagcggc cattatcctc aacccatatc atgctgctaa
    11281 taatctatcc agtgactgct gagcaataac caaatgctga ccgccaaata accttgcacg
    11341 atttaacagc gtgtagagtt ggtaaaccgg ttgacgttca aggaaatcgg caggtagcgg
    11401 ggatactgac tgatagccgt catagatttg tggcggctgt tcagtatgca gcggtaacat
    11461 cgccaggtcg cactctcggt caccccagta acaggccggg tcgaaaatgt acgggccatc
    11521 cggacccagc gcacagttgc cggaccataa atcgccgtgc aacagagaag gctgcggttg
    11581 atgcgaggca agacgctgct gaatatgctc gacgatggca tcgatattgc cgaaagcgat
    11641 ccctttttct gccgccagtt ccagctgcca gccaatccgt tgttcagcaa aaaaagttga
    11701 ccagcgacgt tgccaggtgt tgggctgtgg agttgtggag agcgcgttat cgaaatcgag
    11761 gccaaattgt ggttggtcgc tccattgatg taaacgcgca atttgctgac caagaataaa
    11821 tgcactatgc gcatccagcg ggcgaggtgg gagataatcc atcaccagaa aactgtagtc
    11881 acggtcagcg ccaactgccc aaaccttagg cacggtgacg gttttactac gcgacagtaa
    11941 ctccagttgg tcggcttcgg cggtaaaacc gggaagcagt tccctttcat cacatttgac
    12001 gaaaaagtca cgtcctgcat agcgcaaatg ccatgcggca tggacttctc cgccaggcag
    12061 ttcattacgc agttctattt cgccttcacc taactgctcg ctcaaaagac gactgattgc
    12121 ctgccacatg atgtctctcc catgttgtct gccagatcat aaggttagcg caagaatgcg
    12181 gtgtaaaaac acgagctaac gcacaccaca gactgattat tgcccaaaaa ccatttattt
    12241 tctgttgcta ttccattcct cccaggtcgt gatggtgatt tcaggatctt tgccggttgc
    12301 actgtgggta agccccgaaa ccagttctct aatttcttct tcactttggg tactgataag
    12361 tccaaaagtg ttagtcccca gctcatggat attgccatca tcatctgtca tggtgagcaa
    12421 aaaaccgtcg cgagtcaggt aattattcaa ctcgttaatt tcagtcaatg tatcctcatg
    12481 caacatgacg gttattacat aacggacaag atcgccactg gccatagttc acccctttgt
    12541 tatcacatga cttttgagca tagtcggaga aacgcgttgc cgacaggttg gtggtgattc
    12601 ccccaatgct gggggaatgt ttttgttagc gggaaaggta agcgtaaatt ttttgcgtat
    12661 cgtcatggga gcacaggcgt gttccctggt tgagtgtggc tgcactcccc gcagctacgc
    12721 caaaacggac catctcttca agagaagcat tttctgccag tttaagtgtc atcgcgccga
    12781 ccatgctgtc accagcgcca acggtactct ggcttttcac cggtggtggc accacctgaa
    12841 tgcagttttc actatcaacg cccagcgccc cttgtggacc cagggaaacg acaacccgtt
    12901 tggccttgcc gctattaacg atttcctgcg cggctttgcg gacatcgtct ggctgggtga
    12961 gttcgcgatt taccagcgca ctgagttctt tgtggttagg cttaaccaac tcgatgttac
    13021 caatcgccag tgctgcactt aacgcttcgc cagaactgtc gacgatgcag cggatccctt
    13081 gtttttgcgc agcggaaatc agttgggtta atttttccag cttcacacct ggcggcaggc
    13141 ttccgcttat gaccaggatg gccccggatt caatttccag aacttgctct tcgagctggc
    13201 gaaactcatc ttcatttaat gccgcgcctg gcataacaaa acgatactgc tcaccgcttg
    13261 cttccacatg tacgtgtaaa ttctgccggg tccagtcttt ggcttctaca gtagcgacgg
    13321 ggacattttc atccgccaac agtgaaacca gatgttcgcc ggtcgcgcca cccgccggga
    13381 agatcgctgt ggcagtgcct cccagatggg caatggcgcg ggcgacgttg atgccgccgc
    13441 ctccgggttc gaataccggt gcggtacagc gcagttttcc ttcgggataa atttgcgggg
    13501 taattgttgc gctatcgagt gagggcgcaa gtgtcaacgt atagatacgt accatcattt
    13561 cctcctatag gctgatttca gtctggcacc gaatcagcgg aaag
//
LOCUS       DAHHTG010000113        13366 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.114, whole genome shotgun sequence.
ACCESSION   DAHHTG010000113 DAHHTG010000000
VERSION     DAHHTG010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13366)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13366)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13366
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.114"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            101..1600
                     /gene="pitB"
                     /locus_tag="NBI74_003477"
     CDS             101..1600
                     /gene="pitB"
                     /locus_tag="NBI74_003477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitB"
                     /protein_id="HCD1933759.1"
                     /translation="MLNLFVGLDIYTGLLLLLALAFVLFYEAINGFYDTANAVATVIY
                     TRAMQPQLAVVMAAFFNFFGVLLGGLSVAYAIVHMLPTDLLLNMGSTHGLAMVFSMLL
                     AAIIWNLGTWFFGLPASSSHTLIGAIIGIGLTNALLTGSSVMDALNLREVTKIFSSLI
                     VSPIVGLVIAGGLIFLLRRYWSGTKKRDRIHRIPEDRKKKKGKRKPPFWTRIALIVSA
                     AGVAFSHGANDGQKGIGLVMLVLVGIAPAGFVVNMNASGYEITRTRDAVTNFEHYLQQ
                     HPELPQKLITMEPPLPAASTDGAQVTEFHCHPANTFDAIARVKTMLPGNMESYEPLSV
                     SQRSQLRRIMLCISDTSAKLAKLPGVSKEDQNLLKKLRSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVAMTIGEKIGKRGMTYAQGMAAQMTAAVSIGLASYIGMPASTTHVL
                     SSAVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAIFLSGGLYWIALQLI"
     gene            complement(1649..2341)
                     /locus_tag="NBI74_003478"
     CDS             complement(1649..2341)
                     /locus_tag="NBI74_003478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate kinase"
                     /protein_id="HCD1933760.1"
                     /translation="MQSITPPLIAVIGSDGSGKSTVCEHLITVVEKYGAAERVHLGKQ
                     AGNVGRAVTKLPLMGKSLHKTIERNQVKTAKKLPGPVPALVITAFVARRLLRFRHMLA
                     CRRRGLIVLTDRYPQDQIPGAYDGTVFPPNVEGGRFVSWLASQERKAFHWMASHKPDL
                     VIKLNVDLDVACARKPDHKRESLARKIAITPQLTFGGAQLVDIDANQPLEQVLVDAEK
                     AITDFMTARGYH"
     gene            2515..3228
                     /locus_tag="NBI74_003479"
     CDS             2515..3228
                     /locus_tag="NBI74_003479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate kinase"
                     /protein_id="HCD1933761.1"
                     /translation="MNQRNMSIINSTPVRVIAIVGCDGSGKSTLTASLVNELAARMPT
                     ERIYLGQSSGRIGEWISQLPVIGAPFGRYLRSKAAHVHEKPSTPPGNITALVIYLLSC
                     WRAYKFRKMLCKSQQGFLLITDRYPQVEVPGFRFDGPQLAKTTGGNGWIKMLRQRELK
                     LYQWMASYLPVLLIRLGIDEQTAFARKPDHQLAALQEKIAVTPQLTFNGAKILELDGR
                     QPADEIMQASLRAIHAALS"
     gene            3260..4018
                     /locus_tag="NBI74_003480"
     CDS             3260..4018
                     /locus_tag="NBI74_003480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1933762.1"
                     /translation="MDALQTQTVISTTAPQPNYIPGLIAVVGCDGTGKSTLTTDLVKS
                     LQQHWQTERRYLGLLSGEDGDKIKRLPLVGVWLERRLAAKSSKTQSMKTKSPALWAAV
                     IMYCFSLRRMANLRKVQRLAQSGVLVVSDRFPQAEISGFYYDGPGIGVERATGKISMF
                     LAQRERRLYQQMAQYRPELIIRLGIDIETAISRKSDHDYAELQDKIGVMSTIGYNGTK
                     ILEIDSRAPYSEVLEQAQKAVSLVAIVSDRRSLT"
     gene            4064..5413
                     /locus_tag="NBI74_003481"
     CDS             4064..5413
                     /locus_tag="NBI74_003481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HCD1933763.1"
                     /translation="MAGFNIKHWFADGAFRTIIRNSAWLGSSNVVSALLGLLALSCAG
                     KGMTPAMFGVLVIVQSYAKSISDFIKFQTWQLVVQYGTPALTNNNPQQFRNVVSFSFS
                     LDIVSGAVAIVGGIALLPFLSHSLGLDDQSFWLAALYCTLIPSMASSTPTGILRAVDR
                     FDLIAVQQATKPFLRAAGSVVAWYFDFGFAGFVIAWYVSNLVGGTMYWWFAARELRRR
                     NIHNAFKLNLFESARHIKGAWSFVWSTNIAHSIWSARNSCSTVLVGIVLGPAAAGLFK
                     IAMTFFDAAGTPAGLLGKSFYPEVMRLDPCTTRPWLLGVKSGLLAGGIGILVALAVFI
                     VGKPLISLVFGVKYLEAYDLIQVMLGAIVISMLGFPQESLLLMAGKQRAFLVAQTIAS
                     IGYIVLLFMFCHLFGVLGAAFAYFGGQCLDVALSLIPTLKAFFQRHSLLYNAAGEKS"
     gene            5413..6237
                     /locus_tag="NBI74_003482"
     CDS             5413..6237
                     /locus_tag="NBI74_003482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1933764.1"
                     /translation="MMRKYFPLEASERLFVAIEEDDVVDAQVSLPPTIALSCTTEIIH
                     DNYALCLQFWLNGVDRQELLRLVRKQAKGDELTADERKQFKYMRARYKHLRFAQRLYL
                     KKHQAGFLFGKTTVFLGRFQDGFRNGKKNIVPYYGNLLRIYLSSPVWSLVNYSLRHSQ
                     LESVSSFIAYRQKQMHTLKEIIAKPRLTGREFHDVRKIISQQVSYYDTLRSLDPENKE
                     ALQISRFLAAINGLMGDKHDDMVADDMENRQSYDAPLALDSDIRQRLELLISRFPL"
     gene            6249..6809
                     /locus_tag="NBI74_003483"
     CDS             6249..6809
                     /locus_tag="NBI74_003483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HCD1933765.1"
                     /translation="MSSRLIIALIIMLLAPGVQAHNFVTGKTVTPVYIQEGGELLLNS
                     DDEIHYQKWNSTQLAGKVRIIQYIAGRKSAKKKNSLLIKAVEAANFPQDRFQPTTIVN
                     TDDAIFGTGYFVVGKIEKNKRRYPWAQFIIDGNGQGRVAWRLPEQSSTILVLNKAGQI
                     QWAKDGSLTPEEVDHVIALAQKLINE"
     gene            6840..7910
                     /gene="lptF"
                     /locus_tag="NBI74_003484"
     CDS             6840..7910
                     /gene="lptF"
                     /locus_tag="NBI74_003484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HCD1933766.1"
                     /translation="MKLVEHYIMRGTRRLVLIIVGFLIFIFASYSAQRYLTEAANGTL
                     ALDVVLDIVFYKVLIALEMLLPVGLYVSVGVTLGQMYTDSEITAISAAGGSPGRLYKA
                     VLYLAIPLSIFVTLLSMYGRPWAYTQIYQLEQQSQSELDVRQLRAKKFNTNDNGRMIL
                     SQTVDQDNNRLTDALIYTSTANRTRIFRARSVDVVDPSPEKPTVMLHNGTAYLLDHQG
                     RDDNEQIYRNLQLHLNPLDQSPNVKRKAKSVTELARSAFPADHAELQWRQSRGLTALL
                     MALLAISLSRVKPRQGRFSTLLPLTLLFIAIFYGGDVCRTLVANGAIPLIPGLWLVPG
                     LMLMGLLMLVARDFSLLQKFSR"
     gene            7907..8986
                     /gene="lptG"
                     /locus_tag="NBI74_003485"
     CDS             7907..8986
                     /gene="lptG"
                     /locus_tag="NBI74_003485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HCD1933767.1"
                     /translation="MNVFSRYLIRHLFLGFAAAAGLLLPLFTTFNLINELDDVSPGGY
                     RWTQAVLVVLMTLPRTLVELSPFIALLGGIVGLGQLSKNSELTAIRSMGFSIFRIALV
                     ALVAGILSTVSLGAIDEWVASPLQQQALQIKSTATALGEDDDITGNMLWARRGNEFVT
                     VKSLNEQGQPVGVEIFHYRDDLSLESYIYARSATIEDDKTWILHGVNHKKWLNGKETL
                     ETSDNLAWQSAFTSMDLDELSMPGNTFSVRQLNHYIHYLQETGQPSSEYRLALWEKLG
                     QPILTLAMILLAVPFTFSAPRSPGMGSRLAVGVIVGLLTWISYQIMVNLGLLFALSAP
                     VTALGLPVAFVLVALSLVYWYDRQH"
     gene            complement(9022..10194)
                     /locus_tag="NBI74_003486"
     CDS             complement(9022..10194)
                     /locus_tag="NBI74_003486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HCD1933768.1"
                     /translation="MGLYDKYARLAGERLQFSDNGLTPFGTCIDEVYSATEGRIGNKK
                     VILAGTNNYLGLTFNHDAISEGQAALAAQGTGTTGSRMANGSYAPHLALEKEIAEFFN
                     RPTAIVFSTGYTANLGVISALADHNAVVLLDADSHASIYDACSLGGAEIIRFRHNDAK
                     DLERRMVRLGERAKEAIIIVEGIYSMLGDVAPLAEIVDIKRRLGGYLIVDEAHSFGVL
                     GATGRGLAEAVGVEDDVDIIVGTFSKSLASIGGFAVGSEAMEVLRYGSRPYIFTASPS
                     PSCIATVRSSLRTIATQPELRQKLMDNANHLYDGLQKLGYELSSHISPVVPVITGSKE
                     EGLRIWRELISLGVYVNLILPPAAPAGITLLRCSVNAAHSREQIDAIIQAFATLKQ"
     gene            complement(10194..10442)
                     /locus_tag="NBI74_003487"
     CDS             complement(10194..10442)
                     /locus_tag="NBI74_003487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HCD1933769.1"
                     /translation="MVNREIVMDYILSCLQDLVENGVEIKPDSDLVNDLGLESIKVMD
                     LLMMLEDRFDISIPINILLDVKTPAQLMETLLPWLENK"
     gene            complement(10474..11388)
                     /locus_tag="NBI74_003488"
     CDS             complement(10474..11388)
                     /locus_tag="NBI74_003488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001481799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HCD1933770.1"
                     /translation="MNQTVAVTGATGFIGKYIIDNLLARGFHVRALTRTARAHVNDNL
                     TWVRGSLEDTHSLSKLVAGASAVVHCAGQVRGHKEEIFTRCNVDGSLRLMQAAKESCF
                     CQRFLFISSLAARHPELSWYANSKHIAEQRLTAMADEITLGVFRPTAVYGPGDKELKP
                     LFDWMLRGLLPRLGAPDTQLSFLHVTDFAQAVGQWLSAETIQTQTYELCDGVAGGYDW
                     QRIQQLAANVRCGSVRMVGIPLPVLTCLADISTALSRLAGKEPMLTRSKIRELTHADW
                     SASNNRISEDINWFPGISLEHALRNGLF"
     gene            complement(11448..13106)
                     /locus_tag="NBI74_003489"
     CDS             complement(11448..13106)
                     /locus_tag="NBI74_003489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001574155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acyl-AMP ligase"
                     /protein_id="HCD1933771.1"
                     /translation="MSNKIFTHSLPMRYADFPTLVDALDYAALSSAGMNFYDRRCQLE
                     DQLEYQTLKARAEAGAKRLLSLNLKKGERVALIAETSSGFVEAFFACQYAGLVAVPLA
                     IPMGVGQRDSWSAKLQGLLASCQPAAIITGDEWLPLVNAATHNNPELHVLSHAWFKAL
                     PEADVALQRPVPNDIAYLQYTSGSTRFPRGVIITHREVMANLRAISHDGIKLRPGDRC
                     VSWLPFYHDMGLVGFLLTPVATQLSVDYLRTQDFAMRPLQWLKLISKNRGTVSVAPPF
                     GYELCQRRVNEKDLAELDLSCWRVAGIGAEPISAEQLHQFAECFRQVNFDDKTFMPCY
                     GLAENALAVSFSDEASGVVVNEVDRDILEYQGKAVAPGAETRAVSTFVNCGKALPEHG
                     IEIRNEAGMPVAERVVGHICISGPSLMSGYFGDQVSQDEIAATGWLDTGDLGYLLDGY
                     LYVTGRIKDLIIIRGRNIWPQDIEYIAEQEPEIHSGDAIAFVTVQEKIILQIQCRISD
                     EERRGQLIHALAARIQSEFGVTAAIDLLPPHSIPRTSSGKPARA"
BASE COUNT         3219 a         3278 c         3459 g         3410 t
ORIGIN      
        1 actcttttta tcgttaaaaa atgatatttc actttgccca caccgttaaa gtcactgata
       61 atgcgtccgt tcgtaaattc aaaatggcgt aatctaatat atgctaaatt tatttgttgg
      121 ccttgatata tacacagggc ttttgttatt acttgctctg gcatttgtgt tgttctacga
      181 agcaatcaat ggtttttatg atacggcgaa tgcggtggca accgttattt atactcgtgc
      241 catgcaacca caacttgctg tggtgatggc ggcatttttt aacttttttg gcgtgttatt
      301 gggcggactt agcgttgcct atgccattgt ccatatgtta ccaaccgatt tgctgctgaa
      361 tatggggtca actcacggtc tggcgatggt cttttccatg ctgctggcgg cgattatctg
      421 gaacctggga acgtggttct ttggtttacc ggcctccagt tcgcacacct tgattggtgc
      481 gattatcggc atcggtttaa ccaacgcgct gttaaccggc tcatcggtga tggatgcgtt
      541 aaacctgcgt gaagtgacca aaattttctc ctcgctgatt gtttccccta tcgtcggcct
      601 ggtcattgcg ggaggcctga tattcctgct gcgacgctac tggagcggaa cgaaaaagcg
      661 tgaccgtatt caccgcattc cggaagatcg caaaaagaaa aaaggcaaac gtaagccgcc
      721 attctggacg cgtattgcgc tgattgtttc cgctgcgggc gtggcgtttt cgcacggcgc
      781 taacgacggg cagaaaggga tcggcctggt gatgctggta ctggtgggga ttgcccctgc
      841 tggcttcgtc gtcaatatga acgcgtccgg ctatgaaatt acccgtaccc gcgatgccgt
      901 caccaacttc gaacactacc tgcaacagca tcctgaactg ccgcagaagt tgattacgat
      961 ggaacctcca ttgcctgcgg catctactga tggcgcgcaa gtgacggagt ttcactgtca
     1021 tccggcaaat acctttgatg cgattgcgcg cgttaaaacg atgctgccag gcaatatgga
     1081 aagttacgag ccgttaagcg tgagtcagcg cagccagctg cgccgcatta tgctgtgcat
     1141 ctctgatacc tccgcgaagc tggcgaaact gccaggcgtc agtaaagaag accagaacct
     1201 gctgaaaaaa cttcgcagcg atatgttaag caccattgag tacgctccgg tgtggatcat
     1261 catggcggta gcactggcgc tcggcattgg caccatgatt ggctggcgtc gtgtagcgat
     1321 gaccatcggt gagaagattg gtaagcgcgg catgacgtat gcgcaaggca tggcggcaca
     1381 aatgacggcg gcagtgtcta tcggtcttgc cagttatatt gggatgcccg cctccacaac
     1441 acacgtcctc tcgtctgcgg tagcagggac gatggtggtg gacggcggtg ggttacagcg
     1501 taaaacggta accagcatcc tgatggcgtg ggtatttact ttaccggcgg cgatttttct
     1561 ttctggtggg ctgtactgga tagcattgca gttgatttaa ccttcctgaa aatgcccggt
     1621 cctggcgatc gggcatttcc atttttggct agtgataacc acgcgcggtc ataaaatccg
     1681 taatcgcttt ttctgcatca actaacacct gttccagtgg ctgattggcg tcgatatcaa
     1741 ccagttgtgc accaccaaag gttaactgtg gcgttatggc tatctttctt gccagcgatt
     1801 cccttttgtg gtcaggctta cgcgcgcagg ccacgtctag atcgacattg agtttgatga
     1861 ccagatcagg cttatggctc gccatccagt gaaacgcttt acgttcctgg cttgccagcc
     1921 atgagacaaa acgaccacct tcaacgttag gtgggaacac cgtaccatcg taagcgccag
     1981 gaatttggtc ctgaggataa cggtcggtta gaacaattaa cccgcgacga cgacaggcaa
     2041 gcatatgacg aaagcgcagt aagcgacggg cgacaaacgc tgtaattacc agcgccggaa
     2101 ctggtcctgg caattttttt gctgttttca cctgatttcg ttcaattgtt ttatgtaagg
     2161 attttcccat caacggtaat tttgtcactg cacgaccgac atttccggct tgttttccta
     2221 aatgaactct ttcggcagca ccatattttt cgacaacggt aatgagatgt tcacacaccg
     2281 ttgacttgcc tgaaccatcg ctaccaataa cggcaattaa tggaggtgtt attgactgca
     2341 tgaatatatc cttaaattta aatatccatc caaaatattt atttggttaa tatgttttta
     2401 tgaaagcgta attcaggtca atgtcacaat taaccattgt cacaataagg ttgaatggat
     2461 attctgagca cggaccacgt aatattcaat acattattta ctgccgggtt tttcgtgaat
     2521 caacgtaata tgtcaataat taattccacg ccagtgcgtg ttattgccat tgtaggatgt
     2581 gatggttcag gtaaatcgac cctcacggca agcctggtaa atgaactggc agcaagaatg
     2641 ccaacagaac gcatttatct cgggcaatcg tccgggcgaa ttggcgaatg gatttcacag
     2701 ctccctgtta ttggcgcacc ttttgggcgt tatctgcgaa gtaaagcggc acatgtgcac
     2761 gaaaagccct caacaccgcc tggcaatatt actgcgctgg ttatctatct gctttcctgc
     2821 tggcgggcgt acaagtttcg caaaatgttg tgtaaaagcc agcaaggctt tctgctcatc
     2881 accgaccgct acccgcaggt tgaagtgccg ggttttcgct ttgatggccc gcaattggca
     2941 aaaaccacgg gcggtaacgg ttggataaaa atgttaaggc agcgcgagtt gaagctgtac
     3001 caatggatgg cgtcttattt acccgtattg ttgattcgtc ttggcattga tgaacaaacc
     3061 gcgtttgcgc gtaaacctga ccaccaactg gcagcgttac aggaaaaaat cgccgttacg
     3121 ccgcaactga cattcaatgg cgcaaagatc cttgaactgg atggtcggca accagccgat
     3181 gaaattatgc aagcgtcact acgcgcaatt cacgccgcgc tttcctgatc accaaaatgg
     3241 ataggggatg atttaacgaa tggatgcact acaaacacaa actgttatta gtacaaccgc
     3301 gccgcagccc aactacattc cggggctgat tgcggtggtc gggtgtgatg gcaccggtaa
     3361 atccacactg accaccgacc tggtgaaatc gctgcaacaa cactggcaaa ccgagcggcg
     3421 ctatctgggg ctgctctccg gcgaagacgg cgacaaaatc aaacgattgc cgttggttgg
     3481 cgtctggctg gaacggcgac tggcggccaa atcctcgaaa acccaaagca tgaaaaccaa
     3541 atctccggcg ctatgggcgg cggtgattat gtactgcttc tcgctgcgaa gaatggcgaa
     3601 tctacgcaag gttcagcgac tggcgcaaag tggcgttctg gtggtcagcg atcgcttccc
     3661 gcaggctgaa atttcgggct tttattatga tggaccgggg attggcgtcg aacgtgcgac
     3721 cgggaaaatc agcatgtttc tggcgcagcg cgaacggcgt ttataccaac aaatggcgca
     3781 atatcgcccg gaattaatta ttcgcctggg cattgatatt gagactgcca tctcccgcaa
     3841 gtctgaccat gactatgccg agctgcagga caaaatcggc gtcatgtcga cgattggcta
     3901 taacggcaca aaaattcttg agattgattc cagagcgccc tacagcgaag tgctggaaca
     3961 ggcacagaag gcggtttctc tggttgccat cgtttctgac cgccgaagtt taacttaagg
     4021 ctggcgggat actttttact atcaacgata aggtcaggct gaattggcgg gttttaacat
     4081 caaacattgg tttgcagatg gcgcgtttcg caccattatt cgcaatagcg cctggttagg
     4141 ctccagtaat gtcgtgagcg ccttgctggg tctgttggcg ctctcgtgtg ccggtaaagg
     4201 gatgacgccc gccatgtttg gcgtactggt gattgtgcaa tcgtacgcca agtcgatcag
     4261 cgattttatt aagtttcaga catggcaact ggtggttcag tacggaacac cagcattaac
     4321 caacaataat ccgcagcaat tccgcaatgt cgtctcattt tccttctcgc tggatatcgt
     4381 cagcggcgcg gtggcgattg tcggtggcat tgccttactg ccattccttt cccattcatt
     4441 aggtctggat gaccaaagtt tttggctggc agcgctctat tgcacgctca ttccttcaat
     4501 ggcttcctcc acgccgaccg gcattctgcg tgcggtagat cgcttcgatt taattgctgt
     4561 acagcaggcg acgaaacctt ttctgcgcgc agcggggagc gtcgtagcct ggtattttga
     4621 ctttggtttt gcgggttttg ttattgcctg gtacgtgtcg aatctggttg gcggcaccat
     4681 gtactggtgg tttgccgcgc gcgaattacg ccgccgaaat atccataatg ccttcaaatt
     4741 gaatctgttt gagtctgccc gacacatcaa aggcgcgtgg agttttgtct ggtcaaccaa
     4801 cattgcccac tccatctggt cggcgcgtaa ctcgtgcagc acagtgctgg tggggatcgt
     4861 gttaggaccc gctgccgccg ggttatttaa aatcgccatg acattcttcg acgccgccgg
     4921 aacgccagcg ggtttgctgg gtaaaagttt ttacccagag gtgatgcgtt tagatccgtg
     4981 caccaccaga ccgtggttgt taggtgtgaa gtccggttta ctggcgggcg gaatcggtat
     5041 tctggtggcg ctcgcggtgt ttatcgtcgg caagccgctc atttcgctgg tgtttggcgt
     5101 taagtatctc gaagcgtatg acctgattca ggtgatgctg ggcgcaattg tgatttcgat
     5161 gctgggcttc cctcaggagt cattgctatt gatggcggga aaacagcgcg cttttctcgt
     5221 ggcgcaaacc atcgcctcaa tcggctacat cgtattgctg ttcatgttct gtcatctgtt
     5281 tggtgtgctg ggagcagcgt ttgcttactt cggtgggcaa tgtctggacg tggcgctctc
     5341 gttaattcca acgttaaagg cattttttca gcgccattcc ttgctttata acgcagccgg
     5401 agagaaatcc tgatgatgag aaaatatttc ccgcttgagg cgagcgagcg tctatttgtt
     5461 gcgattgaag aagatgatgt cgttgatgca caggttagtt tgccgccgac tattgcacta
     5521 agttgtacca ccgaaatcat tcatgataat tacgcgttat gcctgcaatt ctggctcaat
     5581 ggcgttgatc gtcaggaatt actgcgttta gttcgcaaac aagccaaagg cgatgagctg
     5641 actgctgatg agcgcaaaca atttaaatac atgcgagcgc gctacaagca cctgcgtttt
     5701 gcgcaacggc tgtatttaaa gaagcatcag gccggatttt tattcggcaa aacgaccgtt
     5761 tttctggggc gttttcagga cggctttcgt aacggtaaga aaaatattgt gccgtattac
     5821 ggcaacctgt tacggattta tttaagctct ccggtctggt cgttggtgaa ctattcatta
     5881 cgccatagcc agttagaaag cgtaagcagt tttattgcct atcggcaaaa gcaaatgcat
     5941 accctgaaag agattattgc caaaccgcga ctaaccggca gggaatttca tgatgtgcga
     6001 aaaatcatca gccaacaggt ttcttattac gacaccttaa gatcgcttga tcctgaaaat
     6061 aaagaggcgt tacagatttc ccgtttcctc gccgccatta atggcctgat gggagataaa
     6121 cacgacgaca tggtggcgga tgatatggaa aaccgccagt cgtatgatgc accgttggcg
     6181 ctggatagcg atattcgtca gcgcttagag ctgctgattt cgcgttttcc gctatagccg
     6241 gagcaatgat gtcatctcgt ttgattattg cgttgattat catgctctta gccccgggtg
     6301 tgcaggcaca caatttcgtc actggcaaaa cggttacccc tgtttatatc caggaaggcg
     6361 gcgagctgct tctgaatagc gacgacgaaa ttcactacca aaagtggaac agcacacaat
     6421 tagcgggaaa agtccgcatt attcagtata tcgccgggcg taagtcggcg aagaaaaaga
     6481 actcactgtt aattaaagcg gttgaagcgg caaactttcc gcaggatcgt tttcagccca
     6541 ccaccattgt gaacaccgat gatgccatct ttggtactgg ctattttgtt gttggcaaaa
     6601 tcgaaaaaaa taaacgccgc tatccgtggg cgcaattcat tattgacggc aatggacagg
     6661 gacgcgttgc ctggcgcttg ccggagcaga gttcaaccat tctggtgctg aataaagccg
     6721 ggcaaattca gtgggcaaag gacggcagcc tgacgccgga agaggtagat catgtcattg
     6781 cgctggcgca gaaactgatc aatgaataaa aaattcaaag gattgagggc gttagtgaga
     6841 tgaagctggt tgaacactac atcatgcgtg gtacgcgccg tctggtgctg attatcgtcg
     6901 gttttctgat ctttattttc gccagctact ccgcacagcg ttatctgacc gaagcggcaa
     6961 atgggacatt agctcttgat gttgtgctgg atatcgtttt ctacaaagtg ctgattgcac
     7021 tggagatgtt gttacctgtt ggtctgtatg tgtcagttgg cgtgacgcta gggcagatgt
     7081 ataccgactc ggaaattacc gctatctctg cggcgggggg cagtccggga cgcttgtaca
     7141 aagccgttct ttatctggcg ataccgctaa gtatttttgt cacccttctg tcgatgtatg
     7201 gtcgtccgtg ggcttatacg cagatttatc aactggagca gcagtcgcag tcggagctgg
     7261 atgttcgcca gttgcgggca aagaaattta acactaacga taacggacga atgatccttt
     7321 cgcagacggt tgatcaggat aataatcgcc tgactgacgc gctgatttat acttctactg
     7381 ccaatcgaac ccgcattttc cgcgcccgtt cggttgatgt ggttgaccca tcacccgaga
     7441 aaccgaccgt tatgttgcac aacggcactg cctatcttct cgatcatcag gggcgtgacg
     7501 acaacgaaca gatctaccgt aatctgcaat tacatctgaa tccgctggat caaagcccta
     7561 acgtcaaacg caaagcaaaa tcggtcacgg agctggcgcg ctccgccttt cctgccgatc
     7621 atgccgaact gcaatggcga caaagccgtg gcctgacagc attgttgatg gcgctgctgg
     7681 ccatttcatt aagtcgggta aaaccgcggc aagggcgatt ttcaacatta ttgccactga
     7741 cgttgctgtt tattgccatt ttttatggcg gcgacgtctg ccgtacgctg gtggctaacg
     7801 gcgcgattcc cctcattcct ggtttgtggt tagtacccgg actcatgcta atgggcctgc
     7861 tgatgctggt cgcacgcgac ttctctttgc tgcagaaatt ttcccgatga atgttttcag
     7921 tcgctattta atccgtcatc tctttctcgg ttttgccgcc gccgcagggc tattgctgcc
     7981 gctttttacc accttcaacc tgattaacga actggatgat gtcagcccgg gcggttatcg
     8041 ctggactcag gcggtgctgg tggtgctaat gaccttaccg cgcacactgg tcgaactttc
     8101 gccgtttatc gccttattgg gtgggattgt tggcctgggg cagttatcga aaaacagtga
     8161 gcttaccgcc attcgcagca tggggttttc tatcttccgt attgcactgg tggcgctggt
     8221 tgcagggata ttgtcgactg tttcgttagg cgcgatagat gagtgggtgg cgtcgccatt
     8281 gcagcaacag gcgttgcaaa tcaaatcgac cgccaccgcg ttgggggagg acgatgacat
     8341 taccggcaat atgctgtggg ccaggcgcgg taatgaattt gtgacggtga aatcgctgaa
     8401 cgagcagggc cagccggtgg gcgtggagat atttcattat cgcgacgatc tctcgctcga
     8461 atcttacatt tatgcacgca gtgccaccat tgaagacgac aaaacgtgga tcctgcatgg
     8521 tgtgaatcat aaaaaatggc ttaacggtaa agaaacgctg gaaacatcag ataatcttgc
     8581 ctggcaatcg gccttcacca gtatggatct tgatgagtta tcgatgccgg ggaatacttt
     8641 ttctgtccgt cagcttaatc attacatcca ttatttgcag gaaaccgggc aacccagcag
     8701 cgaataccgc cttgcactgt gggaaaaact ggggcaaccg atcctgaccc tggcgatgat
     8761 tttgctggct gtaccgttta cctttagcgc cccgcgctcg ccagggatgg gtagccgtct
     8821 cgctgtaggc gtcatcgtgg gcttactcac ctggatcagc tatcaaatca tggttaatct
     8881 gggattgcta tttgcgttaa gcgcacctgt taccgcgctc ggtttaccgg tagcgtttgt
     8941 gttggtggcg ttgagcctgg tgtattggta tgacagacaa cattaaaccc gccgtggcgc
     9001 aaacaccacg gcgggctcac attactgctt cagagtagca aatgcctgaa tgatggcatc
     9061 gatttgctca cgactgtggg cagcatttac gctacagcgc agcagcgtga tgcctgcagg
     9121 cgcggcaggc ggcaggataa gattcacata cacgcccagc gagataagct cgcgccagat
     9181 gcgtaaacct tcctctttcg aaccggttat tactggcact accgggctaa tatgtgaact
     9241 taactcataa cccagttttt gcagaccgtc atacagatgg ttggcgttat ccatcagctt
     9301 ttgccgcagc tctggttgtg tggcaatagt tcttaacgac gagcgcacgg tcgcaatgca
     9361 ggacggagaa ggagaggcgg taaaaatata cgggcggctg ccgtagcgca gcacttccat
     9421 cgcttccgaa ccgacagcaa aaccgccaat cgaggccaga cttttactga aagtaccaac
     9481 gataatatcc acatcatctt caacgccgac agcttcagcc agtccgcgcc ccgttgcgcc
     9541 taacacgcca aacgaatgcg cttcatcgac aatcaaataa ccaccgagac gacgtttgat
     9601 atcaacaatt tccgccagcg gcgcaacgtc accgagcatg ctgtagatgc cctcaacgat
     9661 gataatcgcc tctttcgccc gttcaccgag gcgaaccatg cgccgttcca gatctttagc
     9721 atcgttatgg cggaaacgaa taatttccgc gccaccgaga gaacaggcgt cataaatact
     9781 ggcgtggctg tcggcatcca gtaacaccac ggcgttatgg tcggcaagtg cgctgatgac
     9841 gcctaaatta gcggtataac cggttgaaaa aacgatagcg gtagggcgat tgaaaaactc
     9901 ggcaatctct ttctctaacg ccaggtgcgg cgcataactg ccgtttgcca tacgagaacc
     9961 ggtggtgccg gtgccttggg ccgctaacgc tgcctgacct tcgctgatgg catcatgatt
    10021 aaaggttaaa ccgagataat tattggtccc ggccagaatc acttttttat tgccgatccg
    10081 gccttcggtg gcggaataca cttcatcaat acaggtgcca aacggtgtta acccgttgtc
    10141 actaaattgc agtcgctcgc cagcaaggcg cgcatattta tcgtatagcc ccattattta
    10201 ttctccagcc atggaagtag agtttccatt aattgcgcag gggttttgac atccagcaga
    10261 atattaatag gaatggaaat atcgaatctg tcttccagca tcattaagag atccatcact
    10321 ttgatcgact ccaggccaag atcgttgacg agatcgctgt cgggcttaat ttccacaccg
    10381 ttttcgacca aatcctgtaa acaggaaagg atgtaatcca ttactatttc acgatttacc
    10441 atagaaacgt taacacacct ttaaacaaga caatcaaaat agcccgttgc gtaatgcgtg
    10501 ttccaggcta atcccgggaa accaattaat atcttcagaa atacggttat tacttgccga
    10561 ccagtcggcg tgggttaatt cacgaatttt cgagcgggtc agcataggtt ctttacctgc
    10621 caggcgactc aacgcggtac tgatatccgc aaggcaggtg agtaccggca gaggaatacc
    10681 aaccattcgc acggaaccgc aacggacgtt ggcggcaagt tgctgtatgc gttgccagtc
    10741 atagccgcca gcgacgccat cgcataattc ataggtttgc gtctgtatag tttcggcgct
    10801 taaccactga cccactgctt gcgcgaaatc ggtgacgtgc agaaaagaga gctgtgtatc
    10861 tggcgcacca agtcgtggca gcaggccgcg cagcatccag tcaaacagcg gttttaactc
    10921 tttatcgccg ggaccataca cggctgtcgg gcgaaaaacg cccagcgtaa tttcgtcagc
    10981 cattgcagtc agccgttgtt cggcgatgtg tttggaattt gcgtaccagg agagctcggg
    11041 atggcgcgcc gccagcgaag agataaacag aaaacgttgg caaaagcagc tctcttttgc
    11101 tgcttgcatc aggcgcaggc tgccgtcaac gttacagcgg gtgaaaatct cttctttgtg
    11161 cccgcgcact tgcccggcgc aatggaccac tgcgctggct ccggcaacca gcttgctaag
    11221 tgaatgagta tcttccagcg aaccgcgcac ccaggtgaga ttatcattga cgtgagcgcg
    11281 ggcagtacgc gtcaatgcgc gaacgtgaaa gccgcgggcg agcaggttat cgataatata
    11341 tttaccgata aacccggtag cgcccgtcac cgcgacagtt tgattcatgc cagggattcc
    11401 tgcacattaa gactggcagc ataagccttc tgataacgtt ttttcgctta cgcacgggca
    11461 ggcttgccgg aggacgttcg gggaatactg tggggcggca acagatcgat agccgcggtt
    11521 acgccaaatt cgctttggat ccgtgccgcc agcgcgtgga taagctgccc gcgacgttct
    11581 tcgtcgctga tccgacactg gatctgcaaa atgatttttt cctggacggt aacaaaagca
    11641 atcgcatcgc cagaatgaat ttccggttct tgttccgcaa tatattcaat atcctgcggc
    11701 cagatattac ggccacgaat aataatcaga tctttaatac gtccggtgac atacagataa
    11761 ccgtccagca gataaccgag gtcgccggtg tctaaccagc ccgtcgcggc aatctcgtct
    11821 tgcgaaacct ggtcgccaaa gtaaccgctc atcaggctgg ggccggagat gcaaatatgg
    11881 cctaccacac gttccgcgac cggcatacct gcttcattgc ggatttcaat gccatgttcc
    11941 ggcaacgctt tgccgcagtt gacgaaagtc gatacggcgc gtgtttcagc acccggcgcg
    12001 acggctttgc cctgatattc gaggatgtcg cgatccactt cgttaaccac aaccccggaa
    12061 gcttcatcag agaagctgac agccagcgca ttttctgcca gtccgtagca cggcatgaaa
    12121 gttttatcgt caaagttgac ctgacggaaa cattcagcga attgatggag ttgttctgcg
    12181 gagatgggtt ctgcaccaat accagcgacg cgccagcagg aaagatccag ttcagcgaga
    12241 tctttttcat tcacgcggcg ctggcacaat tcatagccaa acggcggcgc aacggaaacg
    12301 gtgccgcgat ttttactgat caatttaagc cattgcagag gacgcatggc aaaatcctga
    12361 gtgcgcaaat aatctactga aagttgcgtg gcgacggggg tcaggagaaa gccgaccagt
    12421 cccatatcat ggtagaaagg cagccaggag acgcagcggt cgccagggcg taatttaatg
    12481 ccatcgtggc ttatagcacg tagattagcc atcacttcgc gatgggtgat aatgacgcca
    12541 cggggaaaac gggtgctgcc ggaggtgtac tggaggtagg cgatatcgtt cggaactgga
    12601 cgctggagcg caacatcggc ttccggtaat gccttaaacc aggcgtggct taaaacatgt
    12661 aattcggggt tgttatgcgt cgcggcattg accagtggca accactcatc accagtgata
    12721 atggctgcgg gctggcaact tgccagtaaa ccctggagtt tggcgctcca ggaatcccgc
    12781 tgaccgacgc ccattggaat cgccaacggg acggcgacta agccagcata ctgacaggca
    12841 aaaaaagcct ctacaaaccc gctactcgtt tcggcaatca gcgccacgcg ttcgcctttt
    12901 ttcaggttca gcgataacaa ccgcttcgca ccagcttcgg cacgcgcttt taacgtctga
    12961 tattccagtt gatcttcaag ttggcaacgt ctgtcataaa aattcattcc agcgctactc
    13021 agagcggcat agtccaaagc atcaaccagc gttggaaaat cggcatagcg cataggtagg
    13081 gaatgcgtaa agattttatt agacatatac accacgaaaa aattggatat caaaaataaa
    13141 atgacaataa tgcggtggat tatataggca taaaagataa atgagatagt attttcatca
    13201 gtgtgaaaat gaacattgat ttaaataatg aatttgagtt ttttgttttt attgtgaatg
    13261 atatttaatt tatgaatata ttaagaagga gccttgtccg gtcgccttaa atcgacaaaa
    13321 ggcattgtgg cgcttctgtg tagtctctga ccttatcgta atcatt
//
LOCUS       DAHHTG010000114        13282 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.115, whole genome shotgun sequence.
ACCESSION   DAHHTG010000114 DAHHTG010000000
VERSION     DAHHTG010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13282)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13282)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13282
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.115"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            597..2228
                     /gene="oppA"
                     /locus_tag="NBI74_003490"
     CDS             597..2228
                     /gene="oppA"
                     /locus_tag="NBI74_003490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein OppA"
                     /protein_id="HCD1933772.1"
                     /translation="MTNITKRSLVAAGVLAALMAGNVALAADVPAGVTLAEKQTLVRN
                     NGSEVQSLDPHKIEGVPESNISRDLFEGLLVSDLDGHPAPGVAESWDNKDAKVWTFHL
                     RKDAKWSDGTPVTAQDFVYSWQRSVDPNTASPYASYLQYGHIAGIDEILEGKKPITDL
                     GVKAIDDHTLEVTLSEPVPYFYKLLVHPSTSPVPKAAIEKFGEKWTQPGNIVTNGAYT
                     LKDWVVNERIVLERSPTYWNNAKTVINQVTYLPIASEVTDVNRYRSGEIDMTYNNMPI
                     ELFQKLKKEIPDEVHVDPYLCTYYYEINNQKPPFNDVRVRTALKLGMDRDIIVNKVKA
                     QGDMPAYGYTPPYTDGAKLTQPEWFGWSQEKRNEEAKKLLAEAGYTADKPLTINLLYN
                     TSDLHKKLAIAASSLWKKNIGVNVKLVNQEWKTFLDTRHQGTFDVARAGWCADYNEPT
                     SFLNTMLSNSSMNTAHYKSPAFDSIMAETLKVTDEAQRAALYTKAEQQLDKDSAIVPV
                     YYYVNARLVKPWVGGYTGKDPLDNTYTRNMYIVKH"
     gene            2314..3234
                     /gene="oppB"
                     /locus_tag="NBI74_003491"
     CDS             2314..3234
                     /gene="oppB"
                     /locus_tag="NBI74_003491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppB"
                     /protein_id="HCD1933773.1"
                     /translation="MLKFILRRCLEAIPTLFILITISFFMMRLAPGSPFTGERTLPPE
                     VMANIEAKYHLNDPIMTQYFSYLKQLAHGDFGPSFKYKDYSVNDLVASSFPVSAKLGA
                     AAFFLAVILGVSAGVIAALKQNTKWDYTVMGLAMTGVVIPSFVVAPLLVMIFAIILHW
                     LPGGGWNGGALKFMILPMVALSLAYIASIARITRGSMIEVLHSNFIRTARAKGLPMRR
                     IILRHALKPALLPVLSYMGPAFVGIITGSMVIETIYGLPGIGQLFVNGALNRDYSLVL
                     SLTILVGALTILFNAIVDVLYAVIDPKIRY"
     gene            3249..4157
                     /gene="oppC"
                     /locus_tag="NBI74_003492"
     CDS             3249..4157
                     /gene="oppC"
                     /locus_tag="NBI74_003492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000979653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppC"
                     /protein_id="HCD1933774.1"
                     /translation="MMLSKKNSETLENFSEKLEVEGRSLWQDARRRFMHNRAAVASLI
                     VLVLIALFVILAPMLSQFAYDDTDWAMMSSAPDMESGHYFGTDSSGRDLLVRVAIGGR
                     ISLMVGVAAALVAVVVGTLYGSLSGYLGGKVDSVMMRLLEILNSFPFMFFVILLVTFF
                     GQNILLIFVAIGMVSWLDMARIVRGQTLSLKRKEFIEAAQVGGVSTPGIVIRHIVPNV
                     LGVVVVYASLLVPSMILFESFLSFLGLGTQEPLSSWGALLSDGANSMEVSPWLLLFPA
                     GFLVVTLFCFNFIGDGLRDALDPKDR"
     gene            4169..5182
                     /gene="oppD"
                     /locus_tag="NBI74_003493"
     CDS             4169..5182
                     /gene="oppD"
                     /locus_tag="NBI74_003493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP binding protein OppD"
                     /protein_id="HCD1933775.1"
                     /translation="MSVIETATVPLAQQQADALLNVKDLRVTFSTPDGDVTAVNDLNF
                     SLRAGETLGIVGESGSGKSQTAFALMGLLAANGRIGGSATFNGREILNLPEHELNKLR
                     AEQISMIFQDPMTSLNPYMRVGEQLMEVLMLHKNMSKAEAFEESVRMLDAVKMPEARK
                     RMKMYPHEFSGGMRQRVMIAMALLCRPKLLIADEPTTALDVTVQAQIMTLLNELKREF
                     NTAIIMITHDLGVVAGICDKVLVMYAGRTMEYGNARDVFYQPVHPYSIGLLNAVPRLD
                     AEGETMLTIPGNPPNLLRLPKGCPFQPRCPHAMDICSSAPPLEEFTPGRLRACFKPVE
                     ELL"
     gene            5179..6183
                     /gene="oppF"
                     /locus_tag="NBI74_003494"
     CDS             5179..6183
                     /gene="oppF"
                     /locus_tag="NBI74_003494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP binding protein OppF"
                     /protein_id="HCD1933776.1"
                     /translation="MNAVTEGRKVLLEIADLKVHFEIKDGKQWFWQPPKTLKAVDGVT
                     LRLYEGETLGVVGESGCGKSTFARAIIGLVKATDGHVAWLGKELLGMKPDEWRAVRSD
                     IQMIFQDPLASLNPRMTIGEIIAEPLRTYHPKMSRQEVRERVKAMMLKVGLLPNLINR
                     YPHEFSGGQCQRIGIARALILEPKLIICDEPVSALDVSIQAQVVNLLQQLQREMGLSL
                     IFIAHDLAVVKHISDRVLVMYLGHAVELGTYDEVYHNPLHPYTRALMSAVPIPDPDLE
                     KNKTIQLLEGELPSPINPPSGCVFRTRCPIAGPECAKTRPVLEGSFRHAVSCLKVDPL
                     "
     gene            complement(6236..6565)
                     /locus_tag="NBI74_003495"
     CDS             complement(6236..6565)
                     /locus_tag="NBI74_003495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciU family protein"
                     /protein_id="HCD1933777.1"
                     /translation="MDMDLNNRLTEDETLEQAYDIFLELAADNLDPADVLLFNLQFEE
                     RGGAELFDPAEDWQEHVDFDLNPDFFAEVVIGLADSEDGEINDVFARILLCREKDHKL
                     CHIIWRE"
     gene            complement(6600..8060)
                     /gene="cls"
                     /locus_tag="NBI74_003496"
     CDS             complement(6600..8060)
                     /gene="cls"
                     /locus_tag="NBI74_003496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HCD1933778.1"
                     /translation="MTTVYTLVSWLAILGYWLLIAGVTLRILMKRRAVPSAMAWLLII
                     YILPLVGIIAYLAVGELHLGKRRAERARAMWPSTAKWLNDLKACKHIFAEENSSVAAP
                     LFKLCERRQGIAGVKGNQLQLMTESDDVMQALIRDIQLARHNIEMVFYIWQPGGMADQ
                     VAESLMAAARRGIHCRLMLDSAGSVAFFRSPWPELMRNAGIEVVEALKVNLMRVFLRR
                     MDLRQHRKMIMIDNYIAYTGSMNMVDPRYFKQDAGVGQWIDLMARMEGPIATAMGIIY
                     SCDWEIETGKRILPPPPDVNIMPFEQASGHTIHTIASGPGFPEDLIHQALLTAAYSAR
                     EYLIMTTPYFVPSDDLLHAICTAAQRGVDVSIILPRKNDSMLVGWASRAFFTELLAAG
                     VKIYQFEGGLLHTKSVLVDGELSLVGTVNLDMRSLWLNFEITLAIDDKGFGADLAAVQ
                     DDYISRSRLLDARLWLKRPLWQRVAERLFYFFSPLL"
     gene            8203..8376
                     /locus_tag="NBI74_003497"
     CDS             8203..8376
                     /locus_tag="NBI74_003497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciY family protein"
                     /protein_id="HCD1933779.1"
                     /translation="MKRSRTEVGRWRMQRQASRRKSRWLEGQSRRNMRIHSIRKCILN
                     KQRNSLLFAIYNI"
     gene            complement(8431..9684)
                     /gene="kch"
                     /locus_tag="NBI74_003498"
     CDS             complement(8431..9684)
                     /gene="kch"
                     /locus_tag="NBI74_003498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated potassium channel protein"
                     /protein_id="HCD1933780.1"
                     /translation="MSHWTTFKQTATKLWVTLRHDILALAVFLNGLLIFKTIYGMSVN
                     LLDIFHIKAFSELDLSLLANAPLFMLGVFLVLNSIGLLFRAKLAWAISIILLLIALIY
                     TLHFYPWLKFSIGFCIFTLVFLLILSKDFSHSSAAAGTIFAFISFTTLLFYSTYGALY
                     LSEGFNPRIESLMTAFYFSIETMSTVGYGDIVPVSESARLFTISVIISGITVFATSMT
                     SIFGPLIRGGFNKLVKGNNHTMHRKDHFIVCGHSILAINTILQLNQRGQNVTVISNLP
                     EDDIKQLEQRLGDNADVIPGDSNDSSVLKKAGIDRCRAILALSDNDADNAFVVLSAKD
                     MSSDVKTVLAVSDSKNLNKIKMVHPDIILSPQLFGSEILARVLNGEEINNDMLVSMLL
                     NSGHGIFSDNDEQETKADSKESAQK"
     gene            complement(9984..10280)
                     /locus_tag="NBI74_003499"
     CDS             complement(9984..10280)
                     /locus_tag="NBI74_003499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HCD1933781.1"
                     /translation="MLYVIYAQDKADSLEKRLSVRPAHLARLQLLHDEGRLLTAGPMP
                     AVDSNDPGAAGFTGSTVIAEFESLEAAQAWADADPYVAAGVYEHVSVKPFKKVF"
     gene            10504..11220
                     /gene="tonB"
                     /locus_tag="NBI74_003500"
     CDS             10504..11220
                     /gene="tonB"
                     /locus_tag="NBI74_003500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein TonB"
                     /protein_id="HCD1933782.1"
                     /translation="MTLDLPRRFPWPTLLSVCIHGAVVAGLLYTSVHQVIELPAPAQP
                     ISVTMVAPADLEPPQAVQPPPEPVVEPEPEPEPIPEPPKEAPVVIEKPKPKPKPKPKP
                     VKKVEQPKRDVKPVESRPASPFENTAPARPTSSTATAATSKPVTSVASGPRALSRNQP
                     QYPARAQALRIEGQVKVKFDVTPDGRVDNVQILSAKPANMFEREVKNAMRRWRYEPGK
                     PGSGIVVNILFKINGTTEIQ"
     gene            complement(11260..11658)
                     /gene="yciA"
                     /locus_tag="NBI74_003501"
     CDS             complement(11260..11658)
                     /gene="yciA"
                     /locus_tag="NBI74_003501"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioester hydrolase YciA"
                     /protein_id="HCD1933783.1"
                     /translation="MSTAHNVPQGDLVLRTLAMPADTNANGDIFGGWLMSQMDIGGAI
                     LAKEIAHGRVVTVRVEGMTFLRPVAVGDVVCCYARCVQKGTTSVSINIEVWVKKVASE
                     PIGQRYKATEALFKYVAVDPEGKPRALPVE"
     gene            complement(11763..12302)
                     /locus_tag="NBI74_003502"
     CDS             complement(11763..12302)
                     /locus_tag="NBI74_003502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein A"
                     /protein_id="HCD1933784.1"
                     /translation="MKQFLDFLPLVVFFAFYKIYDIYAATAALIVATAIVLIYSWVRF
                     RKVEKMALITFVLVVVFGGLTLFFHNDEFIKWKVTVIYALFAGALLVSQWVMKKPLIQ
                     RMLGKELTLPQSVWSKLNLAWAVFFILCGLANIYIAFWLPQNIWVNFKVFGLTALTLI
                     FTLLSGIYIYRHMPQEDKS"
     gene            complement(12332..13075)
                     /locus_tag="NBI74_003503"
     CDS             complement(12332..13075)
                     /locus_tag="NBI74_003503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0259 family protein"
                     /protein_id="HCD1933785.1"
                     /translation="MSITAQSVYRDTGNFFRNQFMTILLVSLLCAFITVVLGHVFSPS
                     DAQLAQLNDGVPVSGSSGLFDLVQNMSPEQQQILLQASAASTFSGLIGNAILAGGVIL
                     IIQLVSAGQRVSALRAIGASAPILPKLFILIFLTTLLVQIGIMLVVVPGIIMAILLAL
                     APVMLVQDKMGVFASMRSSMRLTWANMRLVAPAVLSWLLAKTLLLLFASSFAALTPEI
                     GAVLANTLSNLISAVLLIYLFRLYMLIRQ"
     gene            complement(13103..13192)
                     /locus_tag="NBI74_003504"
                     /pseudo
     CDS             complement(13103..13192)
                     /locus_tag="NBI74_003504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460692.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
BASE COUNT         3570 a         3136 c         3260 g         3316 t
ORIGIN      
        1 ctgcttctcc tcaccatcat gttattttcg ccacatcata atcctgggct tgctgaagaa
       61 taattgaaat gatattagta attccactgc ctttggtaga ggaaagtgct aaataataat
      121 caattgttaa attattgtgc atttcactac tggaactgta atcagaaaag agagacatgc
      181 ttaactaacc tctatttgat tgaattgaaa gatgtttgtt aaggcatgga tgcaagctat
      241 agattctggt acggtcaata aaagagaatt gcttaacaat tttgcaaaat gtattggcga
      301 gtaagaaccg catttggtac tttccgggca accgccaggc gattctttat tggtaatgag
      361 aataattaac aattaaagac cgtcgcgaaa gaataatgtg tctcgacagg ggagacacag
      421 tacgaatcga cataaggtga tcgtctgaat caccagaata aataaagtcg gtgatagtaa
      481 tacgtaacga taaagtaacc tgacagcaga aagtctccga gcctgtgcag ggtcccaatc
      541 cgggattaca catgctggtt aataccagta attataatga gggagtccaa aaaacaatga
      601 ccaacatcac caagagaagt ttagtagcag ctggcgtttt ggctgcgctt atggcaggga
      661 atgtcgcgct ggcagctgat gtacccgcag gtgtcacact ggcggaaaaa caaacgctgg
      721 tacgtaacaa tggttcagaa gttcagtcat tagatccgca caaaattgaa ggtgttccgg
      781 agtctaatat cagccgagac ctgtttgaag gcttactggt tagcgatctt gacggccatc
      841 cggcacctgg cgtcgctgaa tcctgggata ataaagacgc gaaagtctgg accttccatt
      901 tgcgtaaaga tgcgaaatgg tctgatggca cgccagtcac agcacaagac tttgtgtata
      961 gctggcaacg ttctgttgat ccaaacactg cttctccgta tgccagttat ctgcaatatg
     1021 ggcatatcgc cggtattgat gaaattcttg aagggaaaaa accgattacc gatctcggcg
     1081 tgaaagctat tgatgaccac acattagaag tcaccttaag tgaacctgtt ccgtacttct
     1141 ataaattact tgttcatcca tcaacttcac cggtgccaaa agccgctatc gagaaattcg
     1201 gcgaaaaatg gacccagcct ggtaatatcg tcaccaacgg tgcctatacc ttaaaagatt
     1261 gggtcgtaaa cgaacgaatc gttcttgaac gcagcccgac ctactggaac aacgcgaaaa
     1321 ccgttattaa ccaggtaacc tatttgccta ttgcttctga agttaccgat gtcaaccgct
     1381 accgtagtgg tgaaatcgac atgacttata acaacatgcc gatcgaattg ttccagaagc
     1441 tgaaaaaaga gatcccggac gaagttcacg ttgatccata cctgtgcact tactattacg
     1501 aaattaacaa ccagaaaccg ccatttaatg acgtgcgtgt gcgtaccgca ctgaaattgg
     1561 gtatggaccg cgatatcatt gttaataaag tgaaagcgca gggcgacatg cccgcctatg
     1621 gttacactcc accgtatact gatggcgcaa aattgactca gccggaatgg tttggctgga
     1681 gccaggaaaa acgtaacgaa gaagcgaaaa aactgctggc tgaagcgggt tataccgcag
     1741 acaaaccgtt gaccatcaac ctgttgtata acacctccga tctgcataaa aagctggcga
     1801 ttgctgcctc ttcattgtgg aagaaaaaca ttggtgtaaa cgtcaaactg gttaaccagg
     1861 agtggaaaac gttcctcgac acccgtcacc agggcacttt tgatgtggcc cgtgcaggct
     1921 ggtgtgcaga ctacaacgaa ccaacttcct tcctgaacac catgctgtcg aacagctcaa
     1981 tgaataccgc gcattataag agcccggcct ttgacagcat tatggcggaa acgctgaaag
     2041 tgactgacga ggcgcagcgt gcagctctgt acactaaagc agaacaacag ctggataagg
     2101 attcggccat tgttcctgtt tattactacg tgaatgcgcg tttggtgaaa ccgtgggttg
     2161 gtggctatac cggcaaagat ccgctggata atacctacac ccggaatatg tacattgtga
     2221 agcactaatg gcaatacgtg gggcaggagt gtcctgctcc acggtgtctg atttttatcg
     2281 cattacagaa ggcacaggcc agaaggtagg gcaatgttaa aatttattct acgtcgctgt
     2341 ctggaagcga ttccgacgct atttattctt attactattt cgttctttat gatgcgcctc
     2401 gcgccgggca gtccttttac cggcgaacgt actttaccac cggaagtgat ggccaatatc
     2461 gaagcgaaat atcatcttaa tgatccgatc atgacacagt atttcagcta cctgaaacaa
     2521 ctggcgcatg gcgatttcgg tccatcgttt aaatataaag attattcggt caatgacctg
     2581 gttgcatcca gttttcccgt ttctgccaaa ctgggagctg cagcattttt tcttgcggta
     2641 atactgggtg ttagtgctgg cgttattgcc gcattaaagc aaaacaccaa atgggactat
     2701 accgtgatgg ggctggcaat gaccggggtt gttatcccca gttttgtggt tgcgccatta
     2761 ttagtcatga tatttgcgat cattttgcat tggctgccgg gcggtggctg gaatggtgga
     2821 gcgcttaaat tcatgatatt gccaatggtg gcgttatcac tcgcctatat cgccagtatt
     2881 gcgcgtatta cccgtggctc tatgattgag gttttacact ccaactttat tcgtactgcc
     2941 cgggcgaaag ggttacctat gcggcggatc attttacgcc acgcattaaa acctgctctg
     3001 ttacccgtgc tctcctatat ggggcccgca tttgtcggca ttattaccgg ttcgatggtc
     3061 atcgaaacca tttatggttt accggggatt gggcaattgt tcgttaatgg cgcattgaac
     3121 cgtgactatt ccttagtgtt aagtctgacc attctggttg gcgcgttaac cattttgttt
     3181 aatgccattg tcgatgtgct atatgcggtt atcgacccga aaatccgtta ctgatactgg
     3241 agctcgcgat gatgttaagt aagaaaaaca gcgagacgct ggaaaatttc agtgaaaagc
     3301 tggaggtcga agggcgcagc ttgtggcagg acgcacgtcg acgttttatg cataaccgtg
     3361 cggcggttgc cagtctgata gtgctggtgc tgatcgcgtt atttgtaatc ctggcaccga
     3421 tgctttcgca gtttgcctat gacgatactg actgggcgat gatgtccagc gccccggata
     3481 tggagtccgg tcactacttt ggtactgact catccggtcg cgacctgctt gtgcgcgttg
     3541 cgattggcgg gcgtatctca ctcatggtcg gtgttgctgc ggcactggtg gcagtggtcg
     3601 tggggacact ttatggttcg ctttccggtt atctgggcgg taaagtggat tcggtaatga
     3661 tgcgtctgct ggaaatcctc aactccttcc cattcatgtt cttcgtcatt ttgctggtga
     3721 cctttttcgg tcaaaacatc ctgctgattt tcgtggcgat tggcatggtt tcctggctgg
     3781 atatggctcg tattgtgcgt gggcaaaccc tgagtctgaa gcgcaaagag tttattgagg
     3841 cggcacaagt tggcggtgta tcgacgccgg gcattgttat tcgccacatt gtgccgaacg
     3901 tactcggtgt ggtggtggtc tatgcgtcgc ttctggtgcc cagcatgatc ctctttgaat
     3961 ctttccttag cttcctggga ttaggtacac aagagccgtt aagcagctgg ggtgcattgc
     4021 tgagtgatgg cgcgaactcg atggaagtct ctccatggtt actgttgttc ccagcgggat
     4081 tcctcgtggt aacgctgttt tgtttcaact ttatcggcga tggcttgcgt gatgccctcg
     4141 acccgaaaga tcgttaagga gtgcagccat gagcgtaatt gaaactgcaa ctgtgccgct
     4201 cgcacaacaa caggctgacg cactgctgaa cgtgaaagat ttgcgtgtca cctttagtac
     4261 cccggatggc gacgtcacgg cggtcaatga tttgaatttt tcgctacgtg ccggagaaac
     4321 gctgggcatc gtaggtgagt ctggttcggg caaatcgcaa accgcattcg cgttgatggg
     4381 cctgttagct gccaacggac gtattggcgg atcggcaacc ttcaatgggc gtgaaatcct
     4441 caatttgcca gagcatgaac tcaataaact gcgagctgaa caaatctcaa tgatttttca
     4501 ggacccaatg acttcgttga atccctatat gcgcgtcggt gagcagttga tggaagtgct
     4561 gatgctgcat aagaacatga gcaaagctga ggcgtttgaa gaatcggtgc ggatgctcga
     4621 tgcggtaaaa atgccggaag cgcgtaaacg catgaaaatg tacccgcacg aattttctgg
     4681 cggcatgcgt cagcgagtca tgattgcgat ggcattgcta tgtcgaccta agctgctgat
     4741 tgcggatgaa cccactactg cgctggacgt caccgtacag gcgcagatca tgacgctatt
     4801 gaatgaactg aagcgggaat ttaataccgc catcattatg attacccacg atcttggcgt
     4861 ggtggcgggg atctgcgaca aagtgctggt aatgtacgcc gggcgcacga tggaatatgg
     4921 caacgcgcgc gatgtctttt atcaacccgt acatccttat tctatcggtt tgctcaacgc
     4981 ggtgccgcgt ctcgatgcgg aaggtgaaac aatgttgact attccgggta atccgccaaa
     5041 cctgctgcga ttaccgaaag gttgcccgtt ccagccacgt tgtccgcatg cgatggatat
     5101 ttgtagtagc gcaccgccgc tggaagagtt tacgcctggc cgtctgcgtg cttgctttaa
     5161 accggtggag gaactgttat gaatgctgta accgaaggaa gaaaagtcct cctcgaaatc
     5221 gccgatctga aagtgcactt tgaaatcaaa gatggcaaac agtggttctg gcaaccgccg
     5281 aaaacgctca aagccgtcga tggtgtaact cttcgcctgt atgaagggga aacattaggt
     5341 gtggtagggg aatcgggatg cggtaagtcc acctttgctc gcgccatcat tggcctggtt
     5401 aaagcgaccg acggtcatgt tgcctggtta ggtaaagagt tgctgggcat gaagcccgat
     5461 gaatggcgtg ccgtacgcag tgatattcag atgattttcc aggatccgtt ggcatcgctg
     5521 aacccgcgta tgaccatcgg cgagatcatc gctgaaccac tgcgtactta tcatccgaaa
     5581 atgtcacgcc aggaagttcg cgagcgcgtg aaggcgatga tgctgaaagt cggtttgttg
     5641 cctaacctga ttaaccgcta tccgcatgag ttctccggtg ggcagtgcca gcgtatcggg
     5701 attgctcgcg ctcttattct tgaaccgaag ctgattatct gtgatgagcc ggtgtcggcg
     5761 ctggacgtgt caattcaggc gcaggtggtc aacctgctcc agcagttgca acgtgagatg
     5821 ggattgtcat taatttttat cgctcacgac ctggccgtgg taaaacacat ttccgatcgt
     5881 gtgctggtga tgtatctcgg ccatgcggta gaactgggga cctatgatga ggtctaccac
     5941 aatccgctac atccttacac cagggcattg atgtcggcag tccccatacc tgatccggat
     6001 ctggagaaga acaaaaccat ccagttactg gaaggggaat taccgtcgcc gatcaacccg
     6061 ccttccggtt gtgttttccg tacccgttgt ccgattgcag gtccggagtg cgccaaaaca
     6121 cgtcctgtgc tggaagggag tttcagacac gccgtttctt gcctgaaagt cgatccgctt
     6181 taaaagcaat aagggctgac agttgtcagc ccttattcac gccaaaagcg attatttatt
     6241 cccgccagat gatatggcaa agtttgtgat ctttttcgcg gcataacaga atgcgcgcga
     6301 aaacatcgtt gatttcacca tcttcactgt ccgccagacc aatcaccact tcggcaaaaa
     6361 agtccgggtt caaatcgaaa tcaacatgtt cctgccagtc ttccgccgga tcgaataact
     6421 ccgcgccgcc gcgctcttca aactgaagat tgaacagcag aacatcagct ggatcgagat
     6481 tgtcggcagc cagttcgaga aaaatatcgt aggcctgctc aagcgtttca tcttcagtca
     6541 ggcgattgtt tagatccata tccatagtca ctacctgttt aacctctgtt ggcgacgttt
     6601 tacagcaacg ggctgaagaa gtaaaacagt cgctcggcga cacgttgcca taatggacgt
     6661 tttagccata aacgggcatc gagcagacga gaacgcgaaa tataatcgtc ctgaacggcg
     6721 gcgaggtctg caccaaaacc tttatcgtcg attgccaggg taatctcgaa atttagccac
     6781 agactacgca tatcaaggtt aacggtgcca accagactta gttctccatc gaccagcacg
     6841 ctcttggtat gcagtaaccc gccttcaaac tgataaattt taaccccagc agccagcagt
     6901 tctgtaaaga atgcgcgact ggcccagccg accagcatcg agtcattttt tcgcggaagg
     6961 ataatactga catccacccc gcgctgcgcc gccgtgcaaa tcgcatgaag taaatcatcg
     7021 cttggcacaa aatagggcgt tgtcatgatc aaatattcac gagccgaata agcagcagtc
     7081 aataatgcct ggtgaatgag atcttccgga aagccggggc cagaagcaat tgtgtgaatg
     7141 gtgtgaccac tggcctgctc aaacggcata atattgacat ctggtggcgg cggcagaata
     7201 cgtttcccgg tttcaatctc ccagtcgcag gaataaataa tccccatcgc ggtggcgatg
     7261 gggccttcca tacgcgccat cagatcaatc cattgcccta cacccgcatc ttgtttgaag
     7321 tagcgagggt cgaccatatt catgctgccg gtgtacgcga tgtaattatc gatcatgatc
     7381 atcttgcgat gttggcgcag gtccatacgg cgtaaaaaca cacgcatcag attgaccttt
     7441 aaggcttcga caacttcaat accggcatta cgcattagct cgggccacgg gctgcggaaa
     7501 aaagccacac tcccggcgga gtcgagcatc aatcggcaat gaataccgcg ccgtgcagcg
     7561 gccattaatg attcagccac ctggtccgcc atgccgccgg gctgccagat ataaaacacc
     7621 atctcaatat tatggcgcgc gagctggatg tcgcggatta acgcctgcat cacatcatct
     7681 gactcggtca tcagttgtag ctgattccct ttgaccccag cgatcccctg acgacgctcg
     7741 caaagcttga ataatggcgc agcgacactg ctattttctt cggcgaagat atgcttacag
     7801 gctttaaggt cgttaagcca ttttgcggtg gaaggccaca tcgctctggc gcgctcagcg
     7861 cggcgtttgc ctaaatggag ctcgccaacg gcaagatagg caataattcc gactaacggc
     7921 agaatgtaaa taatcaacag ccaggccatc gcggagggaa ctgcgcgtcg tttcattaga
     7981 atgcgtaaag ttacgcctgc aatgagcaac cagtatccca gaatggccaa ccaactcacc
     8041 aacgtataaa cggttgtcat agattagaaa tccttttgaa agcgcattgt tatgagttta
     8101 cgcatatgga ttcatctggc aaataaaaac gagggaaaag cactggtcag cgtcaggggc
     8161 gggggtataa tgaccattct gttgttgcat agagtagtta acatgaagcg gagtagaacg
     8221 gaagtggggc gctggcgcat gcagcgtcag gctagccgac gtaaatcgcg ttggcttgag
     8281 gggcagtcgc gccgaaatat gcgtatccac agcatcagga agtgcattct aaacaaacag
     8341 cgtaactcgt tattgtttgc gatctacaat atctaaatgt actgggcacc attgcggtgc
     8401 ccaataagtt tactcttccg tctttaaact ctatttttgc gccgattctt tactgtcagc
     8461 tttcgtttct tgttcatcgt tatcgctgaa aatgccatga ccggagttca acaacattga
     8521 aacgagcata tcattattaa tctcttcacc atttaaaact cgcgccagaa tttcgctgcc
     8581 aaacagttgt ggtgagagaa tgatatccgg atgtaccatc ttaatcttat tcaggttttt
     8641 gctatcactg acggcaagaa ctgttttgac atcactgctc atatcttttg ccgagagtac
     8701 aacaaacgcg ttatctgcat cgttatcact cagcgccaga atggcccggc aacgatcgat
     8761 tcccgctttc tttaacactg aactgtcatt actgtcaccg gggataacat cggcgttatc
     8821 gcctaagcgt tgctcaagtt gcttgatatc atcttcaggc aagttgctga taaccgttac
     8881 gttttgtccg cgttgattca gttgcagaat cgtattgatg gcgagaatcg aatgtccgca
     8941 aacaataaaa tgatctttac gatgcattgt atgattgttt ccttttacaa gtttgttgaa
     9001 tcccccgcgg ataagcggac caaaaattga ggtcatagat gtggcaaaaa cggtaatgcc
     9061 ggaaataatg accgaaatag tgaacaatcg tgctgattca gaaacaggga caatatcgcc
     9121 atagccgacg gttgacatgg tttctatcga aaaatagaac gcggtcatca aactttctat
     9181 tcgcggatta aaaccttcgc ttaaataaag cgcaccgtaa gtggagtaaa acagtaacgt
     9241 cgtgaaacta ataaatgcga aaattgtccc ggctgctgcg ctactgtggg agaagtcttt
     9301 gctcagtatc agcaaaaaca ccagcgtaaa aatgcaaaat ccaatactaa atttcagcca
     9361 gggataaaaa tgcagagtgt aaattagcgc tatcaacaac aaaatgatac tgattgccca
     9421 tgcgagcttt gcccggaaca gtaagccaat ggagttcagg acaagaaata cgccgagcat
     9481 aaatagtggg gcgtttgcca gcaaggagag atccagctct gaaaatgctt tgatatggaa
     9541 aatatcaagc aaattgaccg acataccata gattgtttta aaaataagca atccatttaa
     9601 aaagacggcc agcgcgagaa tgtcgtgccg tagggtcacc cataactttg ttgctgtttg
     9661 tttgaatgta gtccagtgac tcactgatac ctctgtaacc acaattttgt ttaattatct
     9721 caacgtgcac attttctgaa catacatgca gctcgtgaat gtgttaaaca atagtagatg
     9781 actttatccg cagtaatcaa aggttttgag tgagcaactc tgcgtcagga aagggcattt
     9841 tcctgaatat gttcacatca tataaagaaa tacagataaa gttattatct gcttgtggtg
     9901 gtgaatgcac tgaccgacct ataaggaaag gccaaacaag aacacggttg caaaaaccgt
     9961 gcccttaaat attgaatttc tattcagaac actttcttaa atggtttcac tgaaacgtgt
    10021 tcatagactc ctgccgctac gtacgggtca gcatcggccc aggcctgagc tgcttccagc
    10081 gactcaaatt cagcaataac ggttgagcca gtaaatcccg cagcccctgg atcgttactg
    10141 tctaccgctg gcattggacc ggcggtcaac aaacgacctt catcatgcag taactgtaaa
    10201 cgtgctagat gtgccggacg aacggaaagg cgtttttcga ggctatcagc tttgtcttga
    10261 gcgtaaataa catacaacac aggcacaact ccttgttcgg gaaagttgta aagtacgtta
    10321 tttgaaaggg cgaagatctg caacggaaag atgatgtctt tgttaagacc atgcataaag
    10381 taaggtcaat tacgccaaaa atgacatttt cactgatcct gatcgtcttg ccttattgaa
    10441 tatgattgct atttgcattt aaaatcgggg cctggttttt ctactgaaat gattatgact
    10501 tcaatgaccc ttgatttacc tcgccgcttc ccctggccga cgttactttc ggtctgcatt
    10561 catggtgctg ttgtggcggg tttgctctat acctcggtac atcaggttat tgaactacct
    10621 gcgcctgcgc agccgatttc tgtcacgatg gttgcgcctg ctgatcttga accgccacaa
    10681 gccgttcaac caccaccgga gccggtggta gagccagaac cggaacctga gccgatccct
    10741 gaaccgccaa aagaagctcc ggtggtcatt gaaaagccga agccgaaacc aaagccaaaa
    10801 ccgaagccgg tgaaaaaggt ggagcagcca aaacgcgatg tcaaacccgt agagtcgcgt
    10861 ccggcatcac cgtttgaaaa tacggcaccc gcgcgcccga catcaagtac ggcaacggct
    10921 gcaaccagca agccggttac cagtgtggct tcaggaccac gcgcattaag ccgtaatcag
    10981 ccgcagtatc cggcacgagc acaggcattg cgcattgaag ggcaggttaa agttaaattt
    11041 gatgtcacgc cagatggtcg cgtggataac gtacaaatcc tctcagccaa gcctgcgaac
    11101 atgtttgagc gtgaagtgaa aaatgcgatg cgcagatggc gttatgagcc gggtaagcca
    11161 ggcagtggga ttgtggtgaa tatcctgttt aaaattaacg gcactaccga aattcagtaa
    11221 gcataaagtc aaaagcctcc gaccggaggc ttttgattat tactcaacgg gtaaggcgcg
    11281 aggttttcct tcgggatcaa ccgcgacata cttaaataat gcttctgttg ctttatagcg
    11341 ttgcccgatt ggttctgacg ccactttttt cacccacact tcaatattaa tgctgactga
    11401 tgtcgtccct ttctggacac agcgtgcata gcagcacacc acatcgccga ccgcaaccgg
    11461 ccgtaagaaa gtcattcctt caacccgcac ggtcactaca cgaccgtggg caatttcttt
    11521 cgccagaata gcgccgccaa tatccatttg tgacattaac caaccaccaa agatgtcacc
    11581 attggcattg gtatcggcgg gcatggctaa agtacgtaaa acaagatcgc cctgagggac
    11641 gttatgtgct gtagacatgg taaaaccgac ttaaaaggaa aatcacaggc gcgatgctac
    11701 tatgattttc ctgaggagaa cagaggagat acagcgccag ccccgaagga ctggcagtct
    11761 ggttaggatt tatcttcctg cggcatgtgg cggtagatat agataccgct taacaatgtg
    11821 aagattaagg taagggcggt caggccaaag actttaaagt tgacccaaat attttgcggc
    11881 agccagaatg cgatgtagat gtttgccagg ccgcaaagga taaagaaaac agcccaggcc
    11941 agattcagct tcgaccatac cgattgcggc agggtgagtt ctttacccag catccgctga
    12001 attagcggct ttttcatcac ccattgactg actaacaggg cacccgcaaa cagggcataa
    12061 atgacagtaa ccttccattt aataaactca tcattgtgga agaacagcgt caagccaccg
    12121 aagacgacca ccagaacaaa agtgatcagg gccatcttct caaccttacg aaagcgaacc
    12181 cagctataga taagcacaat cgccgtggcg acgatcagcg ccgcagtagc cgcatagatg
    12241 tcataaatct tgtaaaacgc gaaaaagaca accagcggta aaaaatcaag aaactgcttc
    12301 attttatgat tccgtaatca aatgcttaag gttattggcg aatcaacata tacaggcgga
    12361 acagatagat gagcaatacg gctgaaatca ggttgctcaa ggtgttcgcc agtacggcac
    12421 caatttccgg ggttaatgcg gcaaaagaag aggcaaaaag cagcaacagt gtttttgcca
    12481 gcaaccagct cagtactgcg ggtgccacca gacgcatatt cgcccatgtc agccgcatac
    12541 tgctacgcat cgaggcaaaa acgcccattt tgtcctgtac cagcatcacc ggtgccagag
    12601 ccagtaaaat ggccatgata attcccggaa cgaccaccag catgatgcca atctgtacta
    12661 aaagggtagt cagaaaaatc agaataaata actttggcaa tatcggcgca ctggcaccaa
    12721 tagctcgcag cgcactgact ctctgacccg cagacaccag ctggataatt aatattacgc
    12781 ccccggcgag aatggcgtta ccgattaatc ctgaaaaagt ggacgccgct gaagcctgca
    12841 gcaaaatttg ttgctgttcc ggtgacatat tctgaaccag gtcgaacaac ccactactgc
    12901 cgctaacggg tacgccgtca ttgagctgcg caagctgtgc atcactgggt gagaaaacat
    12961 gccctaacac cactgtgata aacgcacata gcaacgatac caacagaatg gtcataaatt
    13021 gattacggaa gaaatttccg gtgtcacggt atacggactg cgccgtgata gacatgcact
    13081 ctccttgagt tttatgcagg tgttaattag cgggcaattg taccctggtt aagccccggg
    13141 tggcagcatc aggtcttgta tggaaaagca tatctttgta aagcggaggt aattgctggc
    13201 cttcaggcgt gggtaaaccg ctgcaagtgg cgagcgagtt aatatttgcc tagcgaaata
    13261 tataaaaatt gatttaaatc ac
//
LOCUS       DAHHTG010000115        13203 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.116, whole genome shotgun sequence.
ACCESSION   DAHHTG010000115 DAHHTG010000000
VERSION     DAHHTG010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13203)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13203)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13203
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.116"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            <1..282
                     /locus_tag="NBI74_003505"
     CDS             <1..282
                     /locus_tag="NBI74_003505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001204533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCD1933786.1"
                     /translation="ISAQFTSGAQRGGEVHGVFDDPESLGFASSGIRIEGSNPSLFVL
                     TDTVCAVRRGDTLTINGEMFWVDRVSPDDGGSCYLWLNRGQPPAASRRR"
     gene            294..872
                     /locus_tag="NBI74_003506"
     CDS             294..872
                     /locus_tag="NBI74_003506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCD1933787.1"
                     /translation="MAIQGLEQAVENLSRISKTAVPGASAMAINRVASSAISQSASQV
                     ARETKVRRKLVKERARLKRATVKNPQARIKVNRGDLPVIRLGNARVVLSRRRRRKKGQ
                     RSSLKGGDSVLVVGNRRIPGAFIQQLKNGRWHVMQRVAGKKRYPIDVVKIPMAVPLTT
                     AFKQNIERIRRERLPKELGYALQHQLRMVIKR"
     gene            869..1264
                     /locus_tag="NBI74_003507"
     CDS             869..1264
                     /locus_tag="NBI74_003507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCD1933788.1"
                     /translation="MKHTELRAAVLDALEKHDTGATLFDGRPAVFDEEDFPAVAVYLT
                     GAEYTGEELDSDTWQAELHIEVFLPAQVPDSELDSWMESRIYPVMSDVPALSDLITSM
                     VASGYDYRRDDDAGLWSSADLTYVITYEM"
     gene            1272..2012
                     /locus_tag="NBI74_003508"
     CDS             1272..2012
                     /locus_tag="NBI74_003508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCD1933789.1"
                     /translation="MPVPNPVMPVKGAGTTLWVYKGNGDPYANPLSDVDWSRLAKVKD
                     LTPGELTAESYDDSYLDDEDADWTATRQGQKSAGDTSFTLAWMPGEQGQQALLAWFNE
                     GDTRAYKIRFPNGTVDVFRGWVSSIGKAVTAKEVITRTVKVTNVGRPSMAEDRSTVTA
                     TTGMTVTPASASVVKGQSTTLTVAFQPEGATDKSFRAVSADKTKATVSVSGMTITVNG
                     VAAGKVNIPVVSGNGEFAAVAEITVTAS"
     gene            2028..2450
                     /locus_tag="NBI74_003509"
     CDS             2028..2450
                     /locus_tag="NBI74_003509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HCD1933790.1"
                     /translation="MFLKTESFEYNGVTVTLSELSALQRIEHLALMKRQAEQAESDSN
                     RKFTVEDAIRTGAFLVAMSLWHNHPKKTQMPSMNEAVKQIEQEVLTTWPTEAISHAEN
                     VVYRLSGMYEFVVNNAPEQAEDAGPAEPVSAGKCSTVS"
     gene            2432..2866
                     /locus_tag="NBI74_003510"
     CDS             2432..2866
                     /locus_tag="NBI74_003510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HCD1933791.1"
                     /translation="MFDGELSFALKLAREMGRPDWRAMLAGMSSTEYADWHRFYSTHY
                     FHDVLLDMHFSGLTYTVLSLFFSDPDMHPLDFSLLNRCEADEEPEGDVLMQKAAGLAG
                     GVRFGPDGNEVIPASPDVADMTEDDVMLMTVSEGIAGGVRYG"
     gene            2859..5420
                     /locus_tag="NBI74_003511"
     CDS             2859..5420
                     /locus_tag="NBI74_003511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HCD1933792.1"
                     /translation="MAEPVGDLVVDLSLDAARFDEQMARVRRHFSGTESDAKKTAAVV
                     EQSMNRQALAAQKAGISVGQYKAAMRMLPAQFTDVATQLAGGQSPWLILLQQGGQVKD
                     SFGGMIPMFRGLAGAITLPMVGATSLAVATGALAYAWYQGNSTLSDFNKTLVLSGHQS
                     GLTADRMLVLSRAGQAAGLTFNQTSESLSALVKAGVSGEAQIVSISQSVARFSSASGV
                     EVDKVAEAFGKLTTDPTSGLTAMARQFHNVTAEQIAYVAQLQRSGDEAGALQAANEAA
                     TKGFDDQTRRLKENMGTLETWADRTARAFKSMWDAVLDIGRPDTAQEMLIKAEAAFKK
                     ADDIWNLRKDDYFVNDEARARYWDDREKARLALEAARKKAEQQSQQDKNAQQQSDTEA
                     SRLKYTEEAQKAYERLQTPLEKYTARQEELNKALKDGKILQADYNTLMAAAKKDYEAT
                     LKKPKQSGVKVSAGDRQEDSAHAALLTLQAELRTLEKHAGANEKISQQRRDLWKAENQ
                     FAVLEEAAQRRQLSAQEKSLLAHKDETLEYKRQLAALGDKVTYQEHLNALAQQADKFA
                     QQQRAKRAAIDAKSRGLTDRQAAREATEQRLKEEYGDNPLALNNVMSEQKKTWVAEDQ
                     LRGSWMAGLKSGWSEWEESATDSMSQVKSAATQTFDGIAQNMAAMLTGSEQNWRSFTR
                     SVLSMMTEILLKQAMVGIVGSIGSAIGGAVGGGASASGGTAIQAAAAKFHFATGGFTG
                     TGGKYEPAGIVHRGEFVFTKEATSRIGVGNLYRLMRGYATGGYVGTPGSMADSRSQAS
                     GTFEQNNHVVINNNGTNGQIGPQALKAVYDMARKGARDEIQAQMRDGGLLSGGGR"
     gene            5417..5746
                     /locus_tag="NBI74_003512"
     CDS             5417..5746
                     /locus_tag="NBI74_003512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCD1933793.1"
                     /translation="MKTFRWKVKPGMDVTSAPSVREVRFGDGYSQRAPAGLNADLKTY
                     SVTLSVPRWEATALESFLAEHGGWKAFLWTPPYGYRQIKVTCAKWSSQVSMLRVEFSA
                     EFKQVVN"
     gene            5746..6444
                     /locus_tag="NBI74_003513"
     CDS             5746..6444
                     /locus_tag="NBI74_003513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HCD1933794.1"
                     /translation="MQDIRQETLNECTRAEQSASVVLWEIDLTEVGGERYFFCNEQNE
                     KGEPVTWQGRQYQAYPIQGSGFELNGKGTSTRPTLTVSNLYGMVTGMVEDLQSLVGGT
                     VVRRKVYARFLDAVNFVNGNSDADPEQEVISRWRIEQCSELSAVSASFVLSTPTETDG
                     AVFPGRIMLANTCTWTYRGDECGYHGPAVADEYDQPTSDITKDKCSKCLSGCKFRNNV
                     GNFGGFLSINKLSQ"
     gene            6450..7193
                     /locus_tag="NBI74_003514"
     CDS             6450..7193
                     /locus_tag="NBI74_003514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCD1933795.1"
                     /translation="MTQTESAILAHARRCAPAESCGFVVRTPEGERYFPCVNISGEPE
                     DYFRMSPEDWLQAEMQGEIVALVHSHPGGLPWLSEADRRLQVQSDLPWWLVCRGTIHK
                     FRCVPHLTGRRFEHGVTDCYTLFRDAYHLAGIEMPDFTREDDWWRHGQNLYLDNLEAA
                     GLYQVPLSAAQPGDVLLCCFGSSVPNHAAIYCGDGELLHHIPEQLSKRERYTDKWQRR
                     THSLWRHRAWHASAFTGIYNDLAAASIFV"
     gene            7130..7762
                     /locus_tag="NBI74_003515"
     CDS             7130..7762
                     /locus_tag="NBI74_003515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000090899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HCD1933796.1"
                     /translation="MARICLYGDLQRFGRRIDLRVKTGAEAIRALATQLPAFRQKLND
                     GWYQVRIAGRDVSTSGLTAQLHETLPDGAVIHIVPRVAGAKSGGVFQIVLGAAAIAGS
                     FFTAGATLAAWGAAIGAGGMTGILFSLGASMVLGGVAQMLAPKARTPTATSTDNGKQN
                     TYFSSLDNMVAQGNVLPVLYGEMRVGSRVVSQEISTADEGDGGQVVVIGR"
     gene            7823..11221
                     /locus_tag="NBI74_003516"
     CDS             7823..11221
                     /locus_tag="NBI74_003516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HCD1933797.1"
                     /translation="MGKGSSKGHTPREAKDNLKSTQLLSVIDAISEGPIEGPVDGLKS
                     VLLNSTPVLDSEGNTNISGVTVVFRAGEQEQTPPEGFESSGSETVLGTEVKYDTPITR
                     TITSANIDRLRFTFGVQALVETTSKGDRNPSEVRLLVQIQRNGGWVTEKDITIKGKTT
                     SQYLASVVVDNLPPRPFNIRMRRMTPDSTTDQLQNKTLWSSYTEIIDVKQCYPNTALV
                     GVQVDSEQFGSQQVSRNYHLRGRILQVPSNYNPQTRQYIGIWDGTFKPAYSNNMAWCL
                     WDMLTHPRYGMGKRLGAADVDKWALYVIGQNCDQSVPDGFGGTEPRITCNAYLTTQRK
                     AWDVLSDFCSAMRCMPVWNGQTLTFVQDRPSDKVWTYNRSNVVMPEDGAPFRYSFSAL
                     KDRHNAVEVNWIDPDNGWETATELVEDTRAIARYGRNVTKMDAFGCTSRGQAHRAGLW
                     LIKTELLETQTVDFSVGAEGLRHVPGDVIEICDDDYAGISTGGRVLAVNSQTRTLTLD
                     REITLPSSGTTLISLVDGQGNPVSVEVQSVTDGVKVKVSRVPDGVAEYSVWGLKLPTL
                     RQRLFRCVSIRENDDGTYAITAVQHVPAKEAIVDNGAHFDGDQSGTVNGVTPPAVQHL
                     TAEVTADSGEYQVLARWDTPKVVKGVSFMLRLTVAADDGSERLVSTARTTETTYRFTQ
                     LALGNYRLTVRAANAWGQQGDPASVSFRIAAPAAPSRIELTPGYFQITATPHLAVYDP
                     TVQFEFWFSEKRVADIRQVETTARYLGTALYWIAASINIKPGHDYYFYIRSVNTVGKS
                     AFVEAVGRASDDAEGYLDFFKGQITESHLGKELLEKVELTEDNASKLEEFSKEWKDAN
                     DKWNAMWGVKIEQTEDGKHYVAGLGLSMEDTEEGKLSQFLVAANRIAFIDPANGNETP
                     MFVAQGNQIFMNEVFLKYLTAPTITSGGNPPVFSLTPDGRLTAKNADISGNVNANSGT
                     LNNVTINQNCRIMGKLSANQIEGDIVKTVGKAFPRNGSYASGTITVTVYDDQAFDRQI
                     VIPPVLFRGGKHENFNSNNQQSYWYSTCKLQVLKNGQEIFQQPATDVSRVFSSVIDMP
                     AGHGHVTLTFNVSSYGANNWTPTTSISDLLVVVMKKSTAGISIS"
     gene            11280..>13203
                     /locus_tag="NBI74_003517"
     CDS             11280..>13203
                     /locus_tag="NBI74_003517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000290541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HCD1933798.1"
                     /translation="MAVQISGVLKDGAGKPIQNCTIQLKAKRNSTTVVVNTVASENPD
                     EAGRYSMDVEYGQYSVILLVEGFPPSHAGTITVYEDSKPGTLNDFLGAMTEEDVMPEA
                     LRRFEVMVEDVARNAEAASQSAAAAKKSETAAASSRNAAKTSETNAGNSAKAAASSKT
                     AAQNAATAAERSETNARASEEASADSEEASRRNAESAAENAGVATTKAREAAADATKA
                     GQKKDEALSAATRAEKAADRAEAAAEVTAEPYANIVPPLPDVWIPFNDSLDMIAGFSP
                     GYKKIAIGDDVVQVASDKQVNFSRASTATYINKSGELKTAEINEPRFECDGLLIEGQR
                     TNYMLNSESPASWGKSSNMDVPETGTDSFGFTYGKFVCNDSLVGQTSAINMASIAATK
                     SVDVSGDNKYVTTSCRFKTERQVRLRIRFDKYDGSATTFLGDAYIDTQTLEINMTGGA
                     AGRITARVRKDKTTGWIFAEATIQAIDGELKIGSQIQYSPEQGGATVSGDYIYLATPQ
                     VENGPCVSSFIISGGSATTRASDLVSIPTRNNLYKLPFTFLLEIHKNWDIAPNAAPRV
                     WDIAANTGQSAIAAINRGSGKLYMSLSNPSGSYVNSAATDVFAEKTTFGCIAKADGHF
                     HVVTNGKAVNEVYCEYN"
BASE COUNT         3133 a         3209 c         4141 g         2720 t
ORIGIN      
        1 atcagtgcgc agttcacctc cggtgcacag cgtggcggcg aggttcatgg cgtttttgac
       61 gatccggagt cgctgggttt tgccagtagt gggatccgta ttgaaggaag taacccgtca
      121 ttatttgtgc ttacggatac ggtttgtgct gtacggcgtg gtgacacgct gaccattaac
      181 ggcgagatgt tctgggtgga ccgtgtttct ccggatgacg gagggagttg ttatctctgg
      241 ctcaaccggg ggcaaccacc tgccgccagc cgtcgtcgct gaaaggggga tgtatggcca
      301 tacagggtct tgagcaggcc gttgaaaacc tcagccgtat cagcaaaacg gcggtgcccg
      361 gtgcctccgc aatggccatt aaccgcgttg cttcatccgc gatatcgcag tctgcgtcac
      421 aggttgcccg tgagacaaag gtacgccgga aactggtaaa ggaaagggcc aggctgaaaa
      481 gggccacggt caaaaatccg caggccagaa tcaaggttaa ccggggggat ttgcccgtaa
      541 tcaggctggg taacgcgcgg gttgtcctgt cccgacgcag gcgtcgtaaa aaggggcagc
      601 gttcatccct gaaaggtggc gacagcgtgc ttgtggtggg aaaccgtcgt attcccggcg
      661 cgtttattca gcaactgaaa aatggccggt ggcatgtcat gcagcgtgtg gccgggaaaa
      721 aacgttaccc cattgatgtg gtgaaaatcc cgatggcagt gccgctgacc acggcgttta
      781 aacagaatat tgagcggata cggcgtgaac gtcttccgaa agagctgggc tatgcgctgc
      841 agcatcaact gaggatggta ataaagcgat gaaacataca gaactccgtg cagccgtact
      901 ggatgcactg gaaaaacatg acaccggggc gacgcttttt gatggtcgcc ccgctgtttt
      961 tgatgaggag gattttccgg cagttgccgt ttatctcacc ggcgctgaat acacgggcga
     1021 agagctggac agcgatacct ggcaggcgga gctgcatatt gaagttttcc tgcctgctca
     1081 ggtgccggat tcagagctgg attcgtggat ggagtcccgg atttatccgg tgatgagcga
     1141 tgtcccggca ctgtcagatt tgatcaccag tatggtggcc agtggctatg actaccggcg
     1201 cgacgatgat gcgggcctgt ggagttcagc cgatctgact tatgtcatta cctatgaaat
     1261 gtgaggacga tatgcctgta ccaaatccag taatgccggt gaaaggggcc gggaccacac
     1321 tgtgggttta taaggggaac ggtgaccctt atgcgaaccc gctttcagac gttgactggt
     1381 cgcgtctggc taaagttaaa gacctgacgc ccggcgaact gaccgctgag tcctatgacg
     1441 acagctatct cgatgatgaa gatgcggact ggactgcgac caggcagggg cagaaatctg
     1501 ccggagatac cagcttcacg ctggcgtgga tgcccggaga gcaggggcag caggcgctgc
     1561 tggcgtggtt taatgaaggg gatacccgtg cctataaaat ccgcttcccg aacggcacgg
     1621 tcgatgtgtt ccgcggctgg gtcagcagta tcggtaaggc ggtgacggcg aaggaagtga
     1681 tcacccgcac ggtgaaagtc accaacgtgg gacgtccgtc gatggcagaa gatcgcagca
     1741 cggtgacggc gacaaccggc atgacggtga cacccgccag tgcttccgta gtgaaagggc
     1801 agagcactac gctgaccgtg gcattccagc cggagggcgc aaccgacaag agcttccgtg
     1861 cggtgtctgc ggataaaaca aaagccaccg tgtcggtcag cggtatgacc atcaccgtga
     1921 acggcgttgc tgcaggcaag gtcaacattc cggttgtatc cggtaatggt gagtttgctg
     1981 cggttgcaga aatcaccgtc accgccagtt aatccggaga gtcagcgatg ttcctgaaaa
     2041 ccgaatcatt tgaatataac ggcgtgaccg tcacgctttc tgaactgtca gccctgcagc
     2101 gtattgagca tctcgccctg atgaaacggc aggcagaaca ggcggagtca gacagcaacc
     2161 ggaagtttac tgtggaagac gccatcagaa ccggcgcgtt tctggtggcg atgtccctgt
     2221 ggcataacca tccgaagaag acgcagatgc cgtccatgaa tgaagccgtt aaacagattg
     2281 agcaggaagt gcttaccacc tggcccacag aggcaatttc tcatgctgaa aacgtggtgt
     2341 accggctgtc cggtatgtat gagtttgtgg tgaataatgc ccccgaacag gcagaggacg
     2401 ccgggcctgc agagcctgtt tctgcgggaa agtgttcgac ggtgagctga gttttgccct
     2461 gaaactggcg cgtgagatgg ggcgacccga ctggcgcgcc atgcttgccg ggatgtcatc
     2521 cacggagtat gccgactggc accgctttta cagtacccat tattttcatg atgttctgct
     2581 ggatatgcac ttttccgggc tgacgtacac cgtgctcagc ctgtttttca gcgatccgga
     2641 tatgcatccg ctggatttca gtctgctgaa ccggtgtgag gctgacgaag agcctgaagg
     2701 tgatgtgctg atgcagaaag cggcagggct tgccggaggt gttcgttttg gtccggacgg
     2761 gaatgaagtt atccccgcct ccccggatgt ggcggacatg acggaggatg acgtaatgct
     2821 gatgacagta tcagaaggga tcgcaggagg agtccggtat ggctgaaccg gtaggcgatc
     2881 tggtcgttga tttaagtctg gatgcggcca gatttgacga gcagatggcc agagtcaggc
     2941 gtcatttttc cggtacggaa agtgatgcga aaaaaacagc ggcagtcgtt gaacagtcaa
     3001 tgaaccgaca ggcgctggct gcacagaaag cggggatttc cgtcgggcag tataaagccg
     3061 ccatgcgtat gctgcctgca cagttcaccg acgtggccac gcagcttgca ggcgggcaaa
     3121 gcccgtggct gatcctgctg caacagggtg gtcaggttaa ggactccttc ggcgggatga
     3181 tccccatgtt cagggggctt gccggtgcga tcaccctgcc gatggtcggg gccacctcgc
     3241 tggcggtggc gaccggtgcg ctggcgtatg cctggtatca gggtaactca accctgtccg
     3301 atttcaacaa aacgctggtc ctttccggtc atcagtcggg tctgacggca gatcgtatgc
     3361 tggtcctgtc cagagccggg caggcggcag ggctgacgtt taaccagacc agcgagtcac
     3421 tcagtgcact ggttaaggcg ggggtaagcg gtgaggctca gattgtgtcc atcagccaga
     3481 gtgtggcgcg tttctcctct gcatccggcg tggaggtgga caaggtcgct gaagccttcg
     3541 ggaagctgac cacagacccg acgtcggggc tgacggcgat ggcacgtcag ttccataacg
     3601 tgacggcgga gcagattgcg tatgttgctc agttgcagcg ttccggcgat gaagccgggg
     3661 cattgcaggc ggcgaacgag gccgcaacga aagggtttga tgaccagacc cgccgcctga
     3721 aagagaacat gggcacgctg gagacctggg cagacaggac cgcacgggca ttcaaatcca
     3781 tgtgggatgc ggtgctggat attggtcgtc ctgataccgc gcaggagatg ctgattaagg
     3841 cagaggccgc gtttaagaaa gcagacgata tctggaatct gcgcaaggat gattattttg
     3901 ttaacgatga agcacgggcg cgttactggg atgatcgtga aaaggcccgt cttgcgcttg
     3961 aagccgcccg aaagaaggct gaacagcaga gtcaacagga caaaaatgcg cagcagcaga
     4021 gcgataccga agcgtcacgg ctgaaatata ccgaagaggc gcagaaggct tacgaacgcc
     4081 tgcagacacc gctggagaaa tataccgccc gccaggaaga actgaacaag gcactgaaag
     4141 acgggaaaat cctgcaggcg gattacaaca cgctgatggc ggcggcgaaa aaggactatg
     4201 aagcgacgct gaaaaagccg aagcagtccg gcgtgaaggt gtctgcgggc gatcgtcagg
     4261 aagacagtgc tcatgctgcc cttctgacgc ttcaggcaga actccggacg ctggagaagc
     4321 atgccggagc gaatgagaaa atcagccagc agcgccggga tttgtggaaa gcggaaaatc
     4381 agttcgcagt actggaggag gcagcacaac gtcgccagct gtctgcacag gagaaatccc
     4441 tgctggcgca taaagatgag acgctggagt acaaacgcca gctggctgca cttggcgaca
     4501 aggttacgta tcaggagcac ctgaacgcgc tggcgcagca ggcggataaa ttcgcacagc
     4561 agcaacgggc aaaacgggcc gccattgatg cgaaaagccg ggggctgact gaccggcagg
     4621 cagcgcggga agccacggaa cagcgcctga aggaggagta tggcgataat ccgctggcgc
     4681 tgaataacgt catgtcagag cagaaaaaga cctgggtggc tgaagaccag cttcgcggga
     4741 gctggatggc aggcctgaag tccggctgga gtgagtggga agagagcgcc acggacagta
     4801 tgtcgcaggt aaaaagtgca gccacgcaga cctttgatgg tattgcacag aatatggcgg
     4861 cgatgctgac cggtagtgag cagaactggc gcagcttcac ccgttccgtg ctgtccatga
     4921 tgacagaaat tctgcttaag caggcaatgg tggggattgt cgggagtatc ggcagcgcca
     4981 ttggcggggc tgttggtggc ggcgcatccg cgtcaggcgg tacagccatt caggccgctg
     5041 cggcgaaatt ccattttgca actggaggat ttacgggaac cggcggcaaa tatgagccag
     5101 cggggattgt tcaccgtggt gaatttgtct tcacgaagga ggcaaccagc cggattggcg
     5161 tggggaatct ctaccggctg atgcgcggct atgccaccgg cggttatgtc ggtacaccgg
     5221 gcagcatggc agacagccgg tcgcaggcgt ccgggacgtt tgagcagaat aaccatgtgg
     5281 tgattaacaa caacggcacg aacgggcaga ttgggccaca ggcgctgaag gctgtgtatg
     5341 acatggcccg caagggtgcc cgtgatgaaa ttcaggcaca gatgcgtgat ggtggcttgt
     5401 tatccggagg tggacgatga aaaccttccg ctggaaagtg aaacccggga tggatgtgac
     5461 atcggctcct tccgtcaggg aggtgcgctt tggtgatggc tattcccagc gtgcgcctgc
     5521 cgggctgaat gccgacctga aaacgtacag cgtgacgctt tctgttcccc gttgggaggc
     5581 cacggcgctg gagtcgtttc tggctgagca cgggggctgg aaggcctttc tgtggacgcc
     5641 gccttatggt tacaggcaga taaaggtgac ctgcgcaaaa tggtcgtcgc aggtcagtat
     5701 gttgcgtgtt gagttcagcg cagagtttaa acaggtggtg aactgatgca ggatatccga
     5761 caggaaacac tgaatgaatg cacccgtgcg gagcagtcgg ccagcgtggt gctctgggaa
     5821 atcgatctga cagaggtcgg tggcgagcgt tattttttct gtaatgagca gaacgaaaaa
     5881 ggtgagccgg tcacctggca ggggcgacag tatcaggcgt atcccattca ggggagcggt
     5941 tttgaactga atggcaaagg caccagtacg cgccccacgc tgacggtttc taacctgtac
     6001 ggtatggtca ccgggatggt ggaagatctg cagagtctgg tcggcggaac ggtggtcagg
     6061 cgtaaggttt acgcccgttt tctggatgcg gtgaatttcg tcaacggaaa cagcgatgcc
     6121 gatccggagc aggaggtgat cagccgctgg cgcattgagc agtgcagcga actgagcgcg
     6181 gtgagtgcct cttttgtact gtccacgccg acggaaacgg acggcgctgt ttttccggga
     6241 cgtatcatgc tggccaacac ctgcacctgg acctatcgcg gcgatgagtg cggttatcac
     6301 ggtccggcgg tcgcggatga atatgaccag ccaacgtccg atatcacgaa ggataaatgc
     6361 agcaaatgcc tgagtggctg taagtttcgc aataacgtcg gcaactttgg cggcttcctt
     6421 tccattaaca aactttcgca gtaaatccca tgacacagac agaatcagcg attctggcgc
     6481 acgcccggcg atgtgcgcca gcggagtcgt gcggcttcgt ggtgagaacg ccggaggggg
     6541 aaagatattt tccctgcgtg aatatctccg gtgagccgga ggattatttc cggatgtcgc
     6601 cggaggactg gctgcaggca gaaatgcagg gtgagatagt cgcgctggtc cacagccacc
     6661 ccggtggtct gccctggctg agtgaggccg accggcggct gcaggtgcag agtgatttgc
     6721 cgtggtggct ggtctgccgg gggacgattc ataagttccg ctgtgtgccg catctcaccg
     6781 ggcggcgctt tgagcacggg gtgacggact gttacacgct gttccgggat gcttatcatc
     6841 tggcggggat tgagatgccg gattttacgc gggaggatga ctggtggcgt cacggtcaga
     6901 atctctatct ggataatctg gaggcagcgg ggctgtatca ggtgccgttg tcagcggcgc
     6961 agccgggcga tgtgctgctg tgctgctttg gttcatcggt gccgaatcat gccgctattt
     7021 actgtggtga tggcgagctg ctgcaccata ttcctgaaca actgagcaaa cgagagaggt
     7081 ataccgacaa atggcagcga cgcacacact ccctctggcg tcaccgggca tggcacgcat
     7141 ctgcctttac ggggatttac aacgatttgg ccgccgcatc gatcttcgtg tgaaaacggg
     7201 ggctgaagct atccgggcgc tggccacaca gctcccggcg tttcgtcaga aactgaatga
     7261 cggctggtat caggtacgga ttgccgggcg ggatgtcagc acgtccggat taacggcgca
     7321 gttacatgag actctgcctg atggcgctgt gattcatatt gttcccagag tcgccggggc
     7381 caagtcaggt ggtgtattcc agattgtcct gggagctgcc gccattgccg gatcattctt
     7441 tactgccgga gccacccttg cagcatgggg ggcagccatt ggggccggtg gtatgaccgg
     7501 catcctgttt tctctcggtg ccagtatggt gctcggtggt gtggcgcaga tgctcgcacc
     7561 gaaagccagg acgcccacag cgaccagcac ggataacggt aagcagaaca cctatttctc
     7621 ttcactggat aacatggttg cccagggcaa tgttttgcct gttctgtacg gtgaaatgcg
     7681 cgtggggtca cgcgtggttt ctcaggagat cagcacggca gacgaagggg acggtggtca
     7741 ggttgtggtg attggtcgct gatgcaaaat gttttatgtg aaaccgcctg cgggcggttt
     7801 tgtcgtttat ggagcgtgag gaatgggtaa aggcagcagt aaggggcata ccccgcgcga
     7861 agcgaaggac aacctgaagt ccacgcagtt gctgagtgtg atcgatgcca tcagcgaagg
     7921 gccgatagaa ggtccggtgg atggattaaa aagcgtgctg ctgaacagta caccggtgct
     7981 ggacagtgag ggaaatacca acatctccgg cgtcacggtg gtgttccggg caggtgagca
     8041 ggagcagaca ccgccggagg gatttgaatc ctccggttcc gagacggtgc tgggtacgga
     8101 agtgaaatat gacacgccga tcacccgcac cattacgtct gcaaacatcg accgtctgcg
     8161 ctttaccttc ggtgtgcagg cactggtgga aaccacttca aagggggacc gtaatccgtc
     8221 ggaagtccgc ctgctggttc agatacagcg taacggtggc tgggtgacgg aaaaagacat
     8281 aaccattaag ggcaaaacca cctcgcagta tctggcctcg gtggtggtgg ataacctgcc
     8341 tccgcgcccg ttcaatatcc ggatgcgcag gatgacaccg gacagcacca cagaccagct
     8401 gcagaacaaa acgctctggt cgtcatacac cgaaatcatc gatgtgaaac agtgctaccc
     8461 gaacacggca ctggtcggcg tgcaggtgga ctcggaacag ttcggcagcc agcaggtgag
     8521 ccgtaattat catcttcgcg ggcgcattct gcaggtgccg tcgaactata acccgcagac
     8581 gcggcaatac atcggtatct gggacgggac gtttaaaccg gcatacagca acaacatggc
     8641 ctggtgtctg tgggatatgc tgacccaccc gcgctacggc atgggtaaac gtcttggtgc
     8701 ggcagatgtg gataaatggg cgctgtatgt catcggccag aattgcgacc agtcggtgcc
     8761 ggacggtttt ggcggcacgg agccgcgcat cacctgtaac gcttacctga ccacacagcg
     8821 caaggcgtgg gatgtgctca gtgatttctg ctcggcgatg cgctgtatgc cggtatggaa
     8881 cgggcagacg ctgacgttcg tgcaggaccg gccatcggat aaggtgtgga cctataaccg
     8941 cagtaatgtg gtgatgccgg aagatggcgc gccgttccgc tacagcttca gcgccctgaa
     9001 ggaccgtcat aatgccgttg aggtgaactg gattgacccg gataacggct gggagacggc
     9061 gacagagctt gtggaggaca cgcgggccat tgcccgttac ggtcgtaatg tcacgaagat
     9121 ggacgccttt ggctgtacca gccgggggca ggcacaccga gccgggctgt ggctgattaa
     9181 aacggagctg ctggaaacgc agaccgtgga ctttagcgtg ggtgctgaag ggcttcgcca
     9241 tgtaccgggc gatgtcattg aaatctgcga tgatgactat gccggtatca gcaccggcgg
     9301 gcgcgtgctg gcggtgaaca gccagacccg gacgctgacg ctcgaccgtg aaatcacgct
     9361 gccatcctcc ggcaccacgc tgataagcct ggttgacggg cagggtaatc cggtgagcgt
     9421 ggaggttcag tccgtcaccg acggcgtgaa ggtgaaagtg agccgggttc ctgacggcgt
     9481 tgccgaatac agcgtgtggg ggctgaagct gccgacgctg cgccagcgcc tgttccgctg
     9541 tgtgagtatc cgtgagaatg acgacggtac gtatgccatc accgccgtgc agcatgtacc
     9601 ggcaaaagag gccatcgtgg ataacggggc gcactttgac ggcgaccaga gcggaacggt
     9661 aaacggtgtc acgccgccag cagtacagca cctgaccgca gaagtcaccg cagacagcgg
     9721 ggaataccag gtgctggcgc gctgggatac gccgaaggtg gtgaaggggg tgagctttat
     9781 gcttcgcctg accgtggcag cggatgacgg cagtgagcgg ctggtcagca cggcccggac
     9841 gacggaaacc acataccgct tcacgcaact ggcgctggga aactacaggc tgacagtccg
     9901 ggcggcaaat gcctgggggc agcagggcga tccggcatcg gtatcgttcc gtattgccgc
     9961 accggcagcg ccgtcgcgga ttgagctgac gccgggctat tttcagatca ccgccacgcc
    10021 gcatcttgcc gtttatgatc cgacggtaca gtttgagttc tggttctcgg aaaagcgggt
    10081 tgcggatatc aggcaggttg aaactacagc ccgctatctt ggcacggcgc tgtactggat
    10141 agctgccagt atcaatatca agccgggcca tgattattat ttttatatcc gcagtgtgaa
    10201 tactgttggc aaatcggcat tcgtggaggc tgtcggtcgg gcgagcgatg atgcggaagg
    10261 ttacctggat tttttcaaag gccagataac cgaatcccat ctcggcaagg agctgctgga
    10321 aaaagtcgag ctgacggagg ataacgccag caaactggag gagttctcga aagagtggaa
    10381 agacgccaac gataaatgga atgccatgtg gggtgtcaaa attgagcaga ccgaagacgg
    10441 caaacattat gtcgcggggc ttggcctcag catggaggat acagaggaag gcaaactgag
    10501 ccagttcctg gttgccgcta accgtatcgc gtttattgac ccggcaaacg ggaatgaaac
    10561 gccgatgttt gtggcgcagg gcaaccagat attcatgaac gaagtgttcc tgaagtatct
    10621 gacggctccc accattacca gcggcggcaa tccgccggta ttttccctga caccggacgg
    10681 gcggctgacg gcgaaaaatg ccgatatcag cggtaacgtg aatgcgaact ccgggacgct
    10741 caataatgtc acgattaacc agaactgtcg gattatggga aaactgtctg ccaaccagat
    10801 tgaaggtgat attgtcaaaa cggtgggaaa agcctttccg agaaatggca gttatgccag
    10861 cggtacaata acggtcactg tgtacgatga ccaggctttt gaccgtcaga tagtaatccc
    10921 acccgttctg tttcgcggtg gtaagcatga aaacttcaac agcaacaacc aacagtcata
    10981 ctggtattca acctgtaagc tgcaggtact gaagaacgga caggaaatct ttcagcaacc
    11041 cgcgacggat gtcagcaggg tattttcatc agtcattgat atgcctgccg gacacggcca
    11101 tgtcaccctg actttcaatg tttcttcata tggtgctaat aactggacgc caacgaccag
    11161 tatcagcgac cttcttgttg ttgtgatgaa gaaatctaca gccggtatca gtatcagttg
    11221 aattttataa cccagatacg ggtgccagaa atggcgcctt ttttatttgt ggagtgaata
    11281 tggcagtaca gatttcaggc gtgctgaaag acggtgcagg aaaaccgata cagaactgca
    11341 ccattcagct caaagcaaaa cgtaacagca ccacggttgt ggtgaacacg gtggcctcag
    11401 aaaatccgga tgaagccgga cgttacagca tggatgtcga gtatggccag tacagcgtta
    11461 tcctgctggt tgaaggtttt ccgccttcac atgccgggac catcaccgtg tatgaggatt
    11521 ctaagccggg gacgctgaat gattttctgg gggccatgac ggaagaggat gtcatgccgg
    11581 aggcattgcg tcgttttgag gtaatggtgg aggatgtggc acgcaacgct gaagccgcct
    11641 ctcagagcgc agcggcagca aagaaatcag aaacagcagc ggcatcgtcc aggaacgcgg
    11701 cgaaaacatc agagacgaat gcaggtaaca gcgcgaaagc ggcagcttct tcaaaaacag
    11761 ccgcacaaaa cgcagcaaca gcggcagaac gttcagagac aaatgcccgt gcgtcagaag
    11821 aagcctccgc agacagtgaa gaggcttccc gccgtaatgc agagtcagcc gctgaaaatg
    11881 ccggagtcgc caccacaaaa gcgcgggagg ctgcagcaga cgcaacaaag gccgggcaga
    11941 aaaaggatga ggctctgtcg gcagcgacac gagctgaaaa ggcggcagac cgcgcagaag
    12001 ccgcagcgga agtgactgca gagccctatg cgaatatagt gccgccgctg cctgatgtgt
    12061 ggataccgtt taacgattca ctggatatga ttgcgggttt ttctccgggc tataaaaaaa
    12121 tagctattgg tgacgatgtg gttcaggtcg ccagtgataa acaggttaat ttcagtcgcg
    12181 catcaacggc aacatatatc aacaaatctg gcgaactgaa aacggcggaa attaatgagc
    12241 cgcgatttga gtgtgatggc ctgcttattg agggacaaag aacgaactac atgctcaatt
    12301 cggaaagtcc agccagctgg gggaagtcat caaacatgga tgtgcccgaa actgggacgg
    12361 atagttttgg ttttacttat ggaaagtttg tctgcaacga ttctctggtt gggcaaactt
    12421 cggctattaa tatggcatca attgctgcaa caaagtcagt tgatgtctca ggcgataaca
    12481 agtacgtgac aacctcatgc cgttttaaaa cagaacgaca ggtaaggtta cgtatacggt
    12541 ttgataagta tgatggtagt gcaacaactt ttcttggcga tgcgtacatt gatacgcaaa
    12601 cgcttgaaat taatatgaca ggtggtgctg ccggcagaat tacggcacga gtcaggaagg
    12661 ataagaccac gggctggatt tttgcagagg caacgattca ggcaattgat ggtgagttaa
    12721 aaataggctc tcagatacag tattctcctg agcagggtgg ggcaacagta tctggtgact
    12781 atatttatct tgccacccca caagtagaga atgggccgtg tgtatcatca tttattattt
    12841 caggaggcag cgcaacgaca agagccagtg atttggttag tatccccacc agaaataatc
    12901 tttataagtt accatttact tttttacttg agattcataa aaactgggat attgcaccaa
    12961 acgccgcacc ccgcgtgtgg gatatagcag ccaataccgg gcaatcagca attgcagcaa
    13021 tcaacagagg tagtggtaag ttatatatga gtctgtcaaa cccttcaggc tcgtatgtta
    13081 atagcgcagc gacagatgta tttgcagaga aaaccacatt tggatgtatt gcaaaagctg
    13141 atggtcactt tcatgtggtg acaaatggta aagcggttaa tgaagtttat tgtgaatata
    13201 atg
//
LOCUS       DAHHTG010000116        13033 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.117, whole genome shotgun sequence.
ACCESSION   DAHHTG010000116 DAHHTG010000000
VERSION     DAHHTG010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13033)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13033)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13033
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.117"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            complement(<1..850)
                     /gene="rarD"
                     /locus_tag="NBI74_003518"
     CDS             complement(<1..850)
                     /gene="rarD"
                     /locus_tag="NBI74_003518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HCD1933799.1"
                     /translation="MDAKQTRQGVLLALAAYFIWGIAPAYFKLIYYVPADEILTHRVI
                     WSFFFMVVLMSICRQWSYLKTLIQTPQKIFMLAVSAVLIGGNWLLFIWAVNNHHMLEA
                     SLGYFINPLVNIVLGMIFLGERFRRMQWLAVILAICGVLVQLWTFGSLPIIALGLAFS
                     FAFYGLVRKKIAVEAQTGMLIETMWLLPVAAIYLFAIADSSTSHMGQNPMSLNLLLIA
                     AGIVTTVPLLCFTAAATRLRLSTLGFFQYIGPTLMFLLAVTFYGEKPGADKMVTFAFI
                     WVALAIF"
     gene            complement(902..1369)
                     /locus_tag="NBI74_003519"
     CDS             complement(902..1369)
                     /locus_tag="NBI74_003519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HCD1933800.1"
                     /translation="MSAVLTAEQALKLVGEMFVYHMPFNRALGMELERYEKEFAQLAF
                     KNQPMMVGNWAQSILHGGVIASALDVAAGLVCVGSTLTRHETISEDELRQRLSRMGTI
                     DLRVDYLRPGRGERFTATSSLLRAGNKVAVARVELHNEEQLYIASATATYMVG"
     gene            1534..2403
                     /gene="pldA"
                     /locus_tag="NBI74_003520"
     CDS             1534..2403
                     /gene="pldA"
                     /locus_tag="NBI74_003520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase A"
                     /protein_id="HCD1933801.1"
                     /translation="MRTLQGWLLPVFMLPMAVYAQEATVKEVHDAPAVRGSIIANMLQ
                     EHDNPFTLYPYDTNYIIYTQTSDLNKEAIASYDWAENARKDEVKFQLSLAFPLWRGIL
                     GPNSVLGASYTQKSWWQLSNSDESSPFRETNYEPQLFLGFATDYNFAGWTLRDVEMGY
                     NHDSNGRSDPTSRSWNRLYTRLMAENGNWLVEVKPWYVVGNTDDNPDITKYMGYYQLK
                     IGYHLGDAVLSAKGQYNWNTGYGGAELGLSYPITKHVRLYTQVYSGYGESLIDYNFNQ
                     TRVGVGVMLNDLF"
     gene            2528..4357
                     /gene="recQ"
                     /locus_tag="NBI74_003521"
     CDS             2528..4357
                     /gene="recQ"
                     /locus_tag="NBI74_003521"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026263.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HCD1933802.1"
                     /translation="MAQAEVLNLESGAKQVLQETFGYQQFRPGQEEIIDTVLSGRDCL
                     VVMPTGGGKSLCYQIPALLLNGLTVVVSPLISLMKDQVDQLQANGVAAACLNSTQTRE
                     QQLEVMTGCRTGQISLLYIAPERLMLDNFLEHLAHWNPVLLAVDEAHCISQWGHDFRP
                     EYAALGQLRQRFPTLPFMALTATADDTTRQDIVRLLGLNDPLIQISSFDRPNIRYMLM
                     EKFKPLDQLMRYVQEQRGKSGIIYCNSRAKVEDTAARLQSKGISAAAYHAGLENNVRA
                     DVQEKFQRDDLQIVVATVAFGMGINKPNVRFVVHFDIPRNIESYYQETGRAGRDGLPA
                     EAMLFYDPADMAWLRRCLEEKPQGQLQDIERHKLNAMGAFAEAQTCRRLVLLNYFGEG
                     RQEPCGNCDICLDPPKQYDGSTDAQIALSTIGRVNQRFGMGYVVEVIRGANNQRIRDY
                     GHDKLKVYGMGRDKSHEHWVSVIRQLIHLGLVTQNIAQHSALQLTEAARPVLRGESSL
                     QLAVPRIVALKPKAMQKSFGGNYDRKLFAKLRKLRKSIADESNVPPYVVFNDATLIEM
                     AEQMPITASEMLSVNGVGMRKLERFGKPFMALIRAHVDGDDEE"
     gene            4422..5042
                     /gene="rhtC"
                     /locus_tag="NBI74_003522"
     CDS             4422..5042
                     /gene="rhtC"
                     /locus_tag="NBI74_003522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709629.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine export protein RhtC"
                     /protein_id="HCD1933803.1"
                     /translation="MLMLFLTVAMVHIVALMSPGPDFFFVSQTAVSRSRKEAMMGVLG
                     ITCGVMVWAGIALLGLHLIIEKMAWLHTLIMVGGGLYLCWMGYQMLRGALKKEVVSAP
                     APQVELAKSGRSFLKGLLTNLANPKAIIYFGSVFSLFVGDNVGTTERWGIFALIIVET
                     LAWFTVVASLFALPQMRCGYQRLAKWIDGFAGALFAGFGIHLIISR"
     gene            complement(5080..5940)
                     /locus_tag="NBI74_003523"
     CDS             complement(5080..5940)
                     /locus_tag="NBI74_003523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000133649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HCD1933804.1"
                     /translation="MTEATTPHDAIFKAFLSRPETARDFIEIHLPPSLVKLCNLDTLH
                     LESSSFVEENLRQYYNDVLYSVETTKGCGYIYVLIEHQSSPDENMAFRMLRYAIATMQ
                     RHLEAGHDYLPLVIPILFYQGKRSPYPWSTNWLDGFPDPDIARDLYFHAFPLVDITLI
                     PDDEIMQHRSMAAFTLVQKHIRQRDMTTLLDKLSRLMILGQMSGQQIRMLINYMALVG
                     EAQDVRTLVHGLAQRVPQQGEELMTLAEELRRDALLNVAKAMLQRGFDLQLIMEMTGL
                     SQEDLYQLQS"
     gene            complement(6099..6719)
                     /gene="rhtB"
                     /locus_tag="NBI74_003524"
     CDS             complement(6099..6719)
                     /gene="rhtB"
                     /locus_tag="NBI74_003524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine/homoserine lactone efflux protein"
                     /protein_id="HCD1933805.1"
                     /translation="MTLEWWFAYLLTSIILSLSPGSGAINTMTTSLNHGYRGAVASIA
                     GLQTGLAIHIVLVGVGLGTLFSRSVIAFEVLKWAGAAYLIWLGIQQWRAAGAIDLKSL
                     ASTQSRRHLFQRAVFVNLTNPKSIVFLAALFPQFIMPQQPQLMQYIVLGVTTIVVDII
                     VMIGYATLAQRIALWIKGPKQMKALNKIFGSLFMLVGALLASARHA"
     gene            6830..7852
                     /gene="pldB"
                     /locus_tag="NBI74_003525"
     CDS             6830..7852
                     /gene="pldB"
                     /locus_tag="NBI74_003525"
                     /EC_number="3.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipase L2"
                     /protein_id="HCD1933806.1"
                     /translation="MFQQQKDWETRENAFAAFTMGPLTDFWRQRDEAEFTGVDDIPVR
                     FVRFRAQHHDRVVVICPGRIESYVKYAELAYDLFHSGFDVLIIDHRGQGRSGRLLADP
                     HLGHVNRFNDYVDDLAAFWQQEVQPGPWRKRYILAHSMGGAISTLFLQRHPGVCDAIA
                     LTAPMFGIVIRMPSFMARQILNWAEAHPRFRDGYAIGTGRWRALPFAINVLTHSRQRY
                     RRNLRFYADDPTIRVGGPTYHWVRESILAGEQVLAGAGDDATPTLLLQAEEERVVDNR
                     MHDRFCELRTAAGHPVEGGRPLVIKGAYHEILFEKDAMRSVALHAIVDFFNRHNSPSG
                     NRSTEV"
     gene            7860..8660
                     /gene="yigL"
                     /locus_tag="NBI74_003526"
     CDS             7860..8660
                     /gene="yigL"
                     /locus_tag="NBI74_003526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar/pyridoxal phosphate phosphatase YigL"
                     /protein_id="HCD1933807.1"
                     /translation="MYQVVASDLDGTLLSPDHTLSPYAKETLKLLTARGINFVFATGR
                     HHVDVGQIRDNLEIKSYMITSNGARVHDLDGNLIFAHNLDRDIASDLFGVVNDNPDII
                     TNVYRDDEWFMNRHRPEEMRFFKEAVFNYALYEPGLLEPEGVSKVFFTCDSHEKLLPL
                     EQAINARWGDRVNVSFSTLTCLEVMAGGVSKGHALEAVAKKLGYSLQDCIAFGDGMND
                     AEMLSMAGKGCIMGSAHQRLKDLHPELEVIGTNAEDAVPHYLRKLYLS"
     gene            8736..9635
                     /gene="bioP"
                     /locus_tag="NBI74_003527"
     CDS             8736..9635
                     /gene="bioP"
                     /locus_tag="NBI74_003527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter"
                     /protein_id="HCD1933808.1"
                     /translation="MALLIITTILWAFSFSFYGEYLAGHVDSYFAVLVRVGLAALVFL
                     PFLRTRGNSLKTVGLYMLVGAMQLGVMYMLSFRAYLYLTVSELLLFTVLTPLYITLIY
                     DIMSKRRLRWGYAFSALLAVIGAGIIRYDQVTDHFWTGLLLVQLSNITFAIGMVGYKR
                     LMETRPMPQHNAFAWFYLGAFLVAVIAWFLLGNAQKMPQTTLQWGILVFLGVVASGIG
                     YFMWNYGATQVDAGTLGIMNNMHVPAGLLVNLAIWHQQPHWPTFITGALVILASLWVH
                     RKWVAPRSSQTADDRRRDCALNE"
     gene            complement(9523..10476)
                     /gene="metR"
                     /locus_tag="NBI74_003528"
     CDS             complement(9523..10476)
                     /gene="metR"
                     /locus_tag="NBI74_003528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MetR"
                     /protein_id="HCD1933809.1"
                     /translation="MIEVKHLKTLQALRNCGSLAAAAATLHQTQSALSHQFSDLEQRL
                     GFRLFVRKSQPLRFTPQGEILLQLANQVLPQISQALQACNEPQQTRLRIAIECHSCIQ
                     WLTPALENFHKNWPQVEMDFKSGVTFDPQPALQQGELDLVMTSDILPRSGLHYSPMFD
                     YEVRLVLAPDHPLAAKTRITPEDLASETLLIYPVQRSRLDVWRHFLQPAGVSPSLKSV
                     DNTLLLIQMVAARMGIAALPHWVVESFERQGLVVTKTLGEGLWSRLYAAVRDGEQRQP
                     ITEAFIRSARNHACDHLPFVKSAERPTYDAPTVRPGSPARL"
     gene            10594..12855
                     /gene="metE"
                     /locus_tag="NBI74_003529"
     CDS             10594..12855
                     /gene="metE"
                     /locus_tag="NBI74_003529"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HCD1933810.1"
                     /translation="MTILNHTLGFPRVGLRRELKKAQESYWAGNSTREELLAVGRELR
                     ARHWDQQKQAGIDLLPVGDFAWYDHVLTTSLLLGNVPQRHQNNDGSVDIDTLFRIGRG
                     RAPTGEPAAAAEMTKWFNTNYHYMVPEFVKGQQFKLTWTQLLEEVDEALALGHKVKPV
                     LLGPVTYLWLGKVKGEQFDRLSLLNDILPVYQQVLAELAKRGIEWVQIDEPALVLELP
                     QAWLDAYKPAYDALQGQVKLLLTTYFEGVTPNLDTITALPVQGLHVDLVHGKDDVAEL
                     HKRLPSDWLLSAGLINGRNVWRADLTEKYAQIKDIVGKRDLWVASSCSLLHSPIDLSV
                     ETRLDAEVKSWFAFALQKCHELALLRDALNSGDTAALAEWSAPIQARRHSTRVHNPAV
                     EKRLAAITAQDSQRANVYEVRAEAQRARFKLPAWPTTTIGSFPQTTEIRTLRLDFKKG
                     NIDANNYRTGIAEHIKQAIVEQERLGLDVLVHGEAERNDMVEYFGEHLDGFVFTQNGW
                     VQSYGSRCVKPPIVIGDVSRPAPITVEWAKYAQSLTDKPVKGMLTGPVTILCWSFPRE
                     DVSRETIAKQIALALRDEVADLEAAGIGIIQIDEPALREGLPLRRSDWDAYLQWGVEA
                     FRINAAVAKDDTQIHTHMCYCEFNDIMDSIAALDADVITIETSRSDMELLESFEEFDY
                     PNEIGPGVYDIHSPNVPSVEWIEALLKKAAKRIPAERLWVNPDCGLKTRGWPETRAAL
                     ANMVQAAQNLRRG"
BASE COUNT         3103 a         3276 c         3479 g         3175 t
ORIGIN      
        1 caaaaattgc cagcgccacc caaataaagg cgaaagtcac catcttatcg gcacccggtt
       61 tttcaccata aaacgtcaca gccagcagga acatcagcgt cggaccaatg tactggaaaa
      121 agcctaacgt tgagagacgc aggcgtgtcg cagctgcagt aaaacacaac agtggcacgg
      181 tggtgacaat accggcggcg atcagcagta aattcagcga catcgggttt tgccccatat
      241 gactggttga gctgtcggca atagcaaaca ggtaaatcgc cgccacgggc agcaaccaca
      301 tagtttcgat taacatgccg gtttgcgctt caacagcaat cttcttgcgc accaggccgt
      361 agaaagcaaa actaaacgcc agccccagcg cgatgatcgg cagtgaacca aaagtccaca
      421 actgaaccaa cacgccacaa atcgccagaa tcaccgccag ccattgcatc cggcggaatc
      481 gctcgccgag gaaaatcatc cccagcacaa tgttcaccag tgggttaata aagtaaccaa
      541 ggctcgcttc cagcatatgg tgattgttca ccgcccagat aaacagtagc cagttgccac
      601 caatcagcac ggcagagact gccagcataa aaattttctg tggcgtctga atcagcgttt
      661 ttaaatagga ccactggcgg caaatgctca tcagcaccac cataaagaaa aacgaccaga
      721 tcacgcgatg cgtcaggatc tcatcggcgg gcacgtagta aatcagtttg aagtacgctg
      781 gcgcaatacc ccaaataaaa taagcggcaa gagcgagtaa tacgccctgc cgcgtttgtt
      841 ttgcatccat cgggaatact catttttaat tggtaacagc agtttacctg cttttatgtc
      901 ttcaacctac catataggtg gcggtggcac tggcaatata aagctgttct tcattgtgta
      961 attcaacgcg ggcgacggcg actttattgc ctgcacgcaa caggctactg gtagcagtaa
     1021 aacgctcgcc tctgcctggg cgcagataat cgacgcgaag atcaatggtc cccatccgcg
     1081 atagccgctg gcgtagttca tcttcactga tggtttcgtg gcgggttaag gtacttccca
     1141 cgcacaccag acctgcggcg acatccagcg ccgacgcaat gaccccgccg tgtaaaatgc
     1201 tttgcgccca gttgcccacc atcatcggct gatttttaaa ggccagctgt gcgaactctt
     1261 tttcgtaacg ctccagttcc atccccaatg cgcggttaaa tggcatgtga taaacaaaca
     1321 tctcacccac taatttgagg gcttgttcag cggtcagtac ggcagacata acatccttac
     1381 acttcattag ttaatgaaat gttgatttta tgcttattta ttgttggttt ctactttagg
     1441 aagggataac taacggctat ggagttaagt atgtaaaata gcccgcagaa aaatattcac
     1501 cttatcaata attcgttacg gagaacacga gcgatgcgga ctctgcaggg ctggttgttg
     1561 ccggtgttta tgttgcctat ggcagtatat gcacaagagg caacggtgaa agaggtgcat
     1621 gacgcgccag cggtgcgtgg cagtattatc gccaatatgc tgcaggagca tgacaatccg
     1681 ttcacccttt atccttatga caccaactac atcatctaca cccaaaccag cgatctgaat
     1741 aaagaagcga ttgccagtta cgactgggcg gaaaacgcgc gtaaggatga agtaaagttc
     1801 cagttgagcc tggcgtttcc gctgtggcgt gggattttgg ggccaaactc tgtactgggc
     1861 gcctcctaca cacagaaatc ctggtggcag ctttctaata gcgatgagtc atcaccgttt
     1921 cgtgaaacta actacgaacc gcaattgttc ctcggttttg ccacggatta caactttgct
     1981 ggctggacgc tgcgcgatgt ggaaatgggc tacaaccacg actcaaacgg gcgttcagac
     2041 ccgacttccc gcagctggaa ccgcctttat acccgcctga tggcagaaaa cggtaactgg
     2101 ttggtagagg tgaagccgtg gtatgtggtg gggaatacgg acgataaccc ggacatcacc
     2161 aaatatatgg gttactacca gcttaaaatc ggctatcacc tcggtgatgc ggtactgagc
     2221 gcgaaagggc agtacaactg gaacaccggt tacggcggcg cggagttagg cttaagctac
     2281 ccgatcacca aacatgtgcg cctttatact caggtttaca gcggttacgg cgaatcgctc
     2341 atcgactata acttcaacca gacccgcgtt ggtgtgggag ttatgctaaa cgacctgttt
     2401 tgatgaatgg ttggcaaatc tggaatccag catccaggat taccctctca gagactaaaa
     2461 gcattgcagt ttctcgcgca ggcgctgaaa atagcgcctg tttttatttc aggcaatcgg
     2521 ggtgaatgtg gcgcaggcgg aagtgttgaa tctggagtcc ggagctaaac aggttttaca
     2581 agaaaccttt ggctaccaac agtttcgccc cggccaggaa gagattatcg acaccgtact
     2641 ttccggtcgc gattgcctgg tcgtcatgcc cactggtggc ggaaaatccc tttgctatca
     2701 aatccctgcc ttattgctaa acggccttac cgtggttgtc tcaccgctga tttcgttgat
     2761 gaaagatcag gtagatcaac tgcaagccaa cggcgtggcg gcggcgtgcc ttaactcgac
     2821 gcaaacccgc gagcagcaac ttgaagtgat gacaggctgt cgcaccgggc aaatcagcct
     2881 gctttatatc gcgccggaac gcctgatgct ggataacttt cttgagcatc tggcgcactg
     2941 gaatccggtg ttattagccg ttgatgaggc gcactgtatc tcccaatggg gtcacgattt
     3001 ccgcccggaa tacgctgccc tcggtcagct gcgtcagcgt tttccgacgc tgccgtttat
     3061 ggcgctgact gccacagccg atgacaccac gcgccaggat atcgtgcgcc tgctggggct
     3121 gaacgatccg ctgattcaaa tcagcagttt tgaccgtccg aatattcgct acatgctgat
     3181 ggagaaattc aaaccgctcg atcagttgat gcgttatgta caggaacaac gcggtaagtc
     3241 ggggattatt tactgtaata gtcgtgcgaa agtagaagac accgctgcgc gcctgcaaag
     3301 caagggaatt agcgcggcgg cctatcatgc cgggctggaa aataatgttc gcgctgatgt
     3361 gcaggaaaaa ttccagcgcg atgacctgca aattgtggtg gcgacggtgg cgttcggcat
     3421 gggcatcaac aagcctaacg ttcgcttcgt ggtgcatttt gatattccgc gcaatatcga
     3481 atcctattat caggaaactg gacgcgccgg gcgtgatggc ctgcccgcgg aagcgatgct
     3541 gttttacgat ccggctgata tggcgtggct gcgccgttgt ctggaagaga agccgcaggg
     3601 gcagttgcag gatatcgaac gtcataaact caatgcgatg ggcgcgtttg ctgaagcgca
     3661 aacttgccgt cgtttggtat tgctgaacta ttttggcgaa gggcgtcagg agccgtgcgg
     3721 gaactgcgat atctgcctcg atccgccaaa acagtacgat ggttcaaccg atgcgcagat
     3781 tgccctttcc accattggtc gtgtgaatca gcggtttggg atgggttatg tggtggaagt
     3841 gattcgcggt gctaataatc agcgtatccg cgactatggt catgacaaac tgaaagtcta
     3901 tggcatgggc cgtgataaaa gccatgaaca ttgggtgagc gtgatccgcc agctgattca
     3961 cctcggcctg gtgacgcaaa atattgccca gcattctgcc ctacaactga cagaggctgc
     4021 gcgtccggtg ctgcgcggcg aatcctcttt gcaacttgcc gtgccgcgta tcgtggcgct
     4081 caaaccgaaa gcgatgcaga aatcgttcgg cggcaactat gatcgcaaac tgttcgccaa
     4141 attacgcaaa ctgcgtaaat cgattgccga tgaaagcaat gtcccgccgt acgtggtgtt
     4201 taacgacgct accttgattg agatggctga acagatgccg atcaccgcca gcgaaatgct
     4261 cagcgttaac ggcgttggta tgcgcaagct ggaacgcttt ggcaaaccgt ttatggcgct
     4321 gattcgtgcg catgtcgatg gtgacgacga agagtagtca gcagcataaa aaagtgccag
     4381 gatgaagact ccgtaaacgt ttccccccgc gagtctaatg tatgttgatg ttattcctca
     4441 ccgttgccat ggtgcacatt gtggcgctta tgagccccgg tcccgatttc ttttttgtat
     4501 ctcagaccgc tgttagtcgt tcccgtaaag aagcgatgat gggcgtgctg ggcattacct
     4561 gcggcgtaat ggtttgggct gggattgcgt tgcttggcct acatttgatt atcgaaaaaa
     4621 tggcctggct gcatacgctg attatggtgg gcggtggcct gtatctctgc tggatgggtt
     4681 accagatgct acgtggtgca ctgaaaaaag aggttgtttc tgcccctgcg ccacaagtcg
     4741 aactggcgaa aagcgggcgc agttttctta agggcttact gaccaatctc gcgaatccta
     4801 aagcgattat ctactttggt tcggtgttct cgctgtttgt cggtgataat gtcggcacca
     4861 ccgaacgctg gggcattttc gcgttgatta ttgtcgaaac actggcgtgg tttaccgtcg
     4921 ttgccagcct gtttgctctg ccgcaaatgc gctgtggtta tcaacgtctg gcgaagtgga
     4981 ttgatggttt tgccggggcg ttatttgccg gatttggcat tcatttgatt atttcgcgtt
     5041 aagcgcttcc cccctgatac gctatcaggg ggaataacat caggattgca attgataaag
     5101 gtcttcctga gaaagcccgg tcatttccat aatcaactgc aaatcaaagc cacgctgtag
     5161 catggctttt gcgacattga gaagggcatc gcgccgaagt tcttccgcta aagtcatcag
     5221 ttcctctcct tgttgaggca ctcgttgtgc cagaccatgt actaatgttc gtacatcctg
     5281 tgcttcacct accagcgcca tgtagtttat caacatgcgt atttgctgtc cgctcatctg
     5341 ccccagaatc attaagcggg ataacttatc taacagcgtt gtcatatccc gttgacggat
     5401 atgcttctgt accagcgtaa aggcggccat gctgcggtgt tgcatgattt cgtcatcagg
     5461 aattagcgta atatcaacca gcggaaaagc atgaaaatag agatcgcgtg caatgtccgg
     5521 atccggaaaa ccatcgagcc agtttgttga ccatggatat gggctacgct ttccctggta
     5581 aaaaagaatg gggatcacca gaggcagata atcatgtccc gcttcaagat gccgctgcat
     5641 ggtggcaata gcatagcgaa gcatacggaa cgccatattt tcatcaggtg aactttgatg
     5701 ctcaatcaac acatagatat atccgcaccc tttcgtcgtc tctactgaat aaagtacgtc
     5761 gttgtaatac tggcgtaggt tttcctctac aaaactgctg gactcgagat ggagcgtatc
     5821 aagattgcag agtttaacta aagaaggcgg cagatgaatt tcaataaagt cacgggccgt
     5881 ttctggtcgg gataaaaaag ccttaaagat tgcgtcatgt ggtgtcgttg cttccgtcat
     5941 ttttcatcat ccgtgggtga aatatggcgg cattctggca ctaacaaaga gaagtaaata
     6001 gtaaataaat taacagatta cgcttattac gagatgcctc gagcgcggat gtaacaatct
     6061 ctcgaggcac attaatttac gtgagcttta ttccagtttt aagcatgcct cgccgatgct
     6121 aacagcgctc ccaccagcat aaataacgag ccgaaaatct tattcagcgc cttcatctgc
     6181 tttggtcctt taatccatag agcaatccgt tgagcaaggg tggcgtaacc gatcatcaca
     6241 ataatatcga ccacaatagt ggtgacgccg agcacgatat actgcatcag ctgcggctgt
     6301 tgcggcatga tgaattgcgg aaatagcgcc gccagaaaca caatactttt gggattggtg
     6361 agattgacaa aaactgcgcg ctggaacaaa tgtcgacgcg attgagtaga ggccagcgat
     6421 ttaagatcaa ttgcgccagc ggcgcgccac tgctggatcc caagccaaat caagtaagct
     6481 gcgcctgccc acttcaacac ttcaaacgca atcactgagc gggaaaatag cgtccccaac
     6541 cccacgccaa ccagcacaat atgaatcgcc agtccggtct gaagcccagc aatagacgcc
     6601 accgcgccgc gataaccgtg gttgagcgag gtggtcatgg tgttgattgc gccagagcct
     6661 ggcgacaggc ttaaaatgat cgatgtcagc aggtaggcaa accaccattc taaggtcatg
     6721 atgaactccc ggtgtgtcta tttttgtgcc acaatacgct actgtcgcag cgttgtgtca
     6781 ggcacgctta aaaaaacgat tttacgtggt ttaagaggca gattacccga tgtttcagca
     6841 gcaaaaagac tgggaaacaa gagaaaacgc gtttgctgct tttaccatgg gaccgctgac
     6901 tgatttctgg cgtcagcgtg atgaagcaga gtttactggt gtggatgaca ttccggttcg
     6961 ctttgtccgt tttcgcgcac agcaccatga ccgggtggta gttatctgcc cggggcgtat
     7021 tgagagctac gtaaaatatg cggaactggc ctacgacctg ttccattcgg ggtttgatgt
     7081 tttaatcatc gaccatcgcg ggcagggacg ttccggtcgc ctgttagccg atccacatct
     7141 cgggcatgtt aatcgcttta atgattatgt tgatgatctg gcggcattct ggcaacagga
     7201 ggttcagccc ggtccgtggc gtaaacgcta tatactggca cattcgatgg gcggtgcgat
     7261 ctccacatta tttctgcaac gccatccagg tgtatgtgac gccattgcgc taactgcgcc
     7321 aatgtttggg atcgtgattc gtatgccgtc atttatggca cggcagatcc tcaattgggc
     7381 cgaagcgcat ccacgtttcc gtgatggcta tgcaataggc accgggcgct ggcgcgcgtt
     7441 gccgtttgct atcaacgtac tgacccacag cagacagcga tatcgacgta acttacgttt
     7501 ctatgctgat gacccaacga ttcgcgtcgg tgggccgacc taccattggg tacgcgaaag
     7561 tattctggct ggcgaacagg tgttagccgg tgcgggtgat gacgccacgc caacgcttct
     7621 cttacaagct gaagaggaac gcgtggtgga taaccgcatg cacgaccgtt tttgtgaact
     7681 acgcaccgct gcgggccatc ctgtcgaagg aggacggccg ttggtaatta aaggtgcgta
     7741 ccatgagatc ctttttgaaa aggacgcaat gcgctcagtc gcgctccacg ctatcgttga
     7801 ttttttcaac aggcataact cacccagcgg aaaccgctct acagaggttt aaatttctta
     7861 tgtaccaggt tgttgcgtct gatttagatg gcacgttact ttctcccgac catacgttat
     7921 ccccttacgc caaagaaact ctgaagctgc tcaccgcgcg cggcatcaac tttgtgtttg
     7981 cgaccggtcg tcaccacgtt gatgtggggc aaattcgcga taatctggag attaagtctt
     8041 acatgattac ctccaatggc gcgcgcgttc acgatctgga tggcaacctg atttttgctc
     8101 ataacctgga tcgcgacatt gccagcgatc tgtttggcgt agtcaacgac aatccggaca
     8161 tcattactaa cgtttatcgc gacgacgaat ggtttatgaa tcgccatcgc ccggaagaga
     8221 tgcgcttttt caaagaagcg gtgttcaatt atgcgctgta tgagccggga ttgctggagc
     8281 cggaaggcgt cagcaaagtg ttcttcacct gcgattccca tgaaaaactt ctgccactgg
     8341 agcaagcgat taacgctcgc tggggcgatc gcgtcaatgt cagtttctct accttaacct
     8401 gcctggaagt catggctggc ggcgtttcaa aaggccatgc cctggaagcg gtagcgaaga
     8461 aactgggtta cagcctgcaa gattgtattg cctttggcga tggaatgaac gatgccgaaa
     8521 tgctgtcgat ggcggggaaa ggctgcatta tgggcagcgc gcatcaacgg ctgaaagatc
     8581 tgcatccaga gctggaagtg attggtacta acgccgaaga cgcagtgccg cattatctgc
     8641 gtaagctcta tttatcgtaa tcgttcttta tttggtcagt tgtcaacctg atacttcgct
     8701 acaatggata ctcgttaatc aaagagtttt ccattgtggc gctacttatc atcaccacga
     8761 ttctgtgggc cttctccttt agcttttatg gcgagtacct tgcggggcac gtcgatagct
     8821 attttgcggt gctggtgcgc gttggtctgg cggcacttgt ttttctgccg tttctgcgta
     8881 cccgtggcaa tagcctgaaa acggtcggcc tgtatatgct ggtgggcgcg atgcagctcg
     8941 gtgtgatgta tatgctgagt ttccgcgcct atctctacct gacggtttct gaactgctgt
     9001 tgttcaccgt actgacgccg ctctacatca cgctgattta tgacatcatg agtaagcgcc
     9061 gtctgcgctg gggctatgct tttagcgcct tgttggcggt gattggtgcc gggattattc
     9121 gctatgatca ggtcaccgac catttctgga ctggcttgtt gctggtgcaa ctctccaata
     9181 tcacttttgc catcggcatg gtgggttaca aacgtctgat ggaaactcgc ccgatgccgc
     9241 agcataacgc ctttgcgtgg ttctatcttg gcgcgttcct ggtggcagtg attgcatggt
     9301 tcttgctggg aaatgcgcag aaaatgccgc aaaccacgtt gcaatggggc attctggtgt
     9361 ttcttggcgt ggtggcttcc gggattggct actttatgtg gaactacggc gcgacgcagg
     9421 tggacgccgg aacgctgggc attatgaata atatgcacgt tccggcaggg ctgctggtaa
     9481 acctggctat ctggcaccaa cagccgcact ggccaacgtt tattacaggc gcgctggtga
     9541 tcctggcctc actgtgggtg catcgtaagt gggtcgctcc gcgctcttca caaacggcag
     9601 atgatcgcag gcgtgattgc gcgctgaacg aataaacgct tctgtaatcg gctgacgctg
     9661 ctcgccatcg cgcacggcgg cgtacagtcg gctccacaag ccttcgccca gggttttggt
     9721 gaccaccaga ccctggcgtt caaaactctc caccacccaa tgaggtagtg cggcaatacc
     9781 catccgtgcg gcaaccatct gaatcaacaa taaggtgtta tcgacgcttt tcagtgacgg
     9841 gctgacgcct gctggctgaa gaaaatgccg ccagacatcc agtcggctac gctgcaccgg
     9901 ataaatcaac agcgtttcgc tggcgagatc ttccggtgta atacgcgttt ttgccgccag
     9961 tggatggtca ggtgctaaca ccagacgtac ttcatagtcg aacatcggcg aataatgcag
    10021 gccactgcgc ggcagaatat cggacgtcat caccagatcc agctcgccct gttgcaacgc
    10081 aggctgcgga tcaaacgtta cgcccgattt aaaatccatc tccacctgcg gccagttctt
    10141 atggaaattt tctaatgcgg gcgtcagcca ctgaatacag ctatggcact ctatggcaat
    10201 gcgcaggcgc gtctgctgcg gttcattgca ggcttgcagt gcctggctaa tttgcggcag
    10261 cacctgattt gccagttgca acaggatttc tccctgcggt gtaaagcgaa gcggctggct
    10321 tttacgcaca aatagccgga agccaaggcg ttgttccaga tcgctaaact ggtgagacag
    10381 ggcggattgc gtttgatgca acgtcgccgc agcggctgcg agtgagccgc agttccgcaa
    10441 cgcttgtagc gttttcaggt gttttacttc gatcatgaaa gtccttcact tcggcatgaa
    10501 taatttgcgc ttgaggaata tacagtaacc gccaattatg gatgtgtaaa catctggacg
    10561 gctaaatact aaaaattcca taaggggcat ataatgacaa ttcttaatca caccctcggt
    10621 ttccctcgcg ttggcctgcg tcgcgagctg aaaaaagcgc aagagagtta ttgggcgggg
    10681 aactccacgc gtgaagaact gctggcggta gggcgtgaat tgcgtgctcg tcactgggat
    10741 caacaaaagc aagcgggtat cgacctgctg ccggtgggcg attttgcctg gtacgatcat
    10801 gtactgacaa ccagtctgct gctgggtaat gttccgcaac gtcatcagaa caacgatggt
    10861 tcggtagata tcgacaccct gttccgtatt ggtcgtggac gtgcaccgac tggcgaacct
    10921 gcggcggcag cggaaatgac caaatggttt aacaccaact atcactacat ggtgccggag
    10981 ttcgttaaag gccaacagtt caaactgacc tggacgcagc tgctggagga agtggacgag
    11041 gcgctggcgc tgggccacaa ggtgaaacct gtgctgctgg ggccggttac ctacctgtgg
    11101 ctgggtaaag tgaaaggtga acagtttgat cgcctgagcc tgctgaacga cattctgccg
    11161 gtttatcagc aagtgctggc agaactagcg aaacgcggca ttgagtgggt acagatcgac
    11221 gaacccgcgc tagtgctgga actgccgcag gcgtggctgg atgcatacaa acccgcttac
    11281 gacgcgctcc agggccaggt gaaactgctg ctgaccacct attttgaagg cgtaacgcca
    11341 aacctcgaca cgattactgc gctgcctgtt cagggtctgc atgtcgatct tgtacatggt
    11401 aaagatgacg ttgctgaact gcacaagcgc ctgccttctg actggctgct gtctgcgggt
    11461 cttatcaatg gtcgtaacgt ctggcgcgcc gatcttaccg agaaatatgc gcaaattaag
    11521 gacattgtcg gcaaacgtga tttgtgggtg gcatcttcct gctcactgct gcacagcccc
    11581 atcgacctga gcgtggaaac gcgtcttgat gcagaagtga aaagctggtt tgccttcgcc
    11641 ctgcaaaaat gtcatgaact ggcattgctg cgcgatgcgc tgaacagtgg tgatacggca
    11701 gctctggcag agtggagcgc tccgattcag gcgcgtcgtc actctactcg tgtacataat
    11761 ccggcagtag aaaagcgtct ggcggcgatc accgcccagg acagtcagcg tgcgaatgtc
    11821 tatgaagtgc gtgctgaagc ccagcgtgcg cgttttaaac tgcccgcgtg gccgaccacc
    11881 acgattggtt ccttcccgca aaccacggag attcgtaccc tgcgtctgga ttttaaaaag
    11941 ggtaatatcg acgccaataa ctaccgcacg ggcattgcgg aacatatcaa gcaggccatt
    12001 gttgagcagg aacgtttggg actggatgtg ctggtacatg gcgaggccga gcgtaatgac
    12061 atggtggaat actttggcga gcatctggat ggctttgtct ttacgcaaaa cggttgggta
    12121 cagagctacg gttcccgctg cgtgaagcca ccgattgtta ttggtgacgt tagccgcccg
    12181 gcaccgatta ccgtggagtg ggcaaaatat gcgcaatccc tgactgataa accggtgaaa
    12241 gggatgttga ctggcccggt gactattctc tgctggtcgt tcccgcgtga agatgtcagc
    12301 cgtgaaacca tcgccaaaca gattgcgctg gcactgcgtg atgaagtggc cgatctggaa
    12361 gccgctggaa ttggcatcat tcagattgac gaaccggcat tgcgcgaagg tttaccactg
    12421 cgtcgcagcg actgggatgc ctatctccag tggggcgtgg aggctttccg tatcaacgcc
    12481 gccgtggcga aagatgacac acaaatccac actcacatgt gttactgcga gttcaacgac
    12541 attatggatt cgattgcggc gttggacgca gacgtcatca ccatcgaaac ctcgcgttcc
    12601 gacatggagt tgctggagtc gtttgaagag tttgattatc caaatgaaat cggtcctggc
    12661 gtctatgaca ttcactcgcc aaacgtaccg agcgtggaat ggattgaagc cttgctgaag
    12721 aaagcggcaa aacgcattcc ggcagagcgt ctgtgggtca acccggactg tggcctgaaa
    12781 acgcgcggct ggccagaaac ccgcgcggca ctggcgaaca tggtgcaggc ggcgcagaat
    12841 ttgcgtcggg ggtaaaatcc aaaccgggtg gtaataccac ccggtctttt ctcattacag
    12901 cgacgtcttc ccagcatact gcgtaaacca ttccagatat cagttcatac cctgtttaag
    12961 aaaatcttct ttattccctt ctcatgaaat aaatttcgtc atttccgcca ttttttctat
    13021 ctgcctctcc atc
//
LOCUS       DAHHTG010000117        12948 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.118, whole genome shotgun sequence.
ACCESSION   DAHHTG010000117 DAHHTG010000000
VERSION     DAHHTG010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12948)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12948)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12948
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.118"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            149..1069
                     /gene="lpxL"
                     /locus_tag="NBI74_003530"
     CDS             149..1069
                     /gene="lpxL"
                     /locus_tag="NBI74_003530"
                     /EC_number="2.3.1.241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) lauroyltransferase"
                     /protein_id="HCD1933811.1"
                     /translation="MTNLPKFSTALLHPRYWLTWLGIGVLWLVVQLPYPVIYRLGCGL
                     GKLALRFMKRRAKIVHRNLELCFPEMSEQERRKMVVKNFESVGMGLMETGMAWFWPDR
                     RIARWTEVIGMEHIRDVQAQKRGILLVGIHFLTLELGARQFGMQEPGIGVYRPNDNPL
                     IDWLQTWGRLRSNKSMLDRKDLKGMIKALKKGEVVWYAPDHDYGPRSSVFVPLFAVEQ
                     AATTTGTWMLARMSGACLVPFVPRRKPDGKGYQLIILPPECSPPLDDAETTAAWMNKV
                     VEKCIMMAPEQYMWLHRRFKTRPEGVPSRY"
     gene            1241..2467
                     /gene="mdtG"
                     /locus_tag="NBI74_003531"
     CDS             1241..2467
                     /gene="mdtG"
                     /locus_tag="NBI74_003531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtG"
                     /protein_id="HCD1933812.1"
                     /translation="MSPCENDTPINWKRNLIVAWLGCFLTGAAFSLVMPFLPLYVEQL
                     GVTGHSALNMWSGIVFSITFLFSAIASPFWGGLADRKGRKLMLLRSALGMGIVMVLMG
                     LAQNIWQFLILRALLGLLGGFVPNANALIATQVPRNKSGWALGTLSTGGVSGALLGPM
                     AGGLLADSYGLRPVFFITASVLILCFFVTLFCIREKFQPVSKKEMLHMREVVTSLKNP
                     KLVLSLFVTTLIIQVATGSIAPILTLYVRELAGNVSNVAFISGMIASVPGVAALLSAP
                     RLGKLGDRIGPEKILITALIFSVLLLIPMSYVQTPLQLGILRFLLGAADGALLPAVQT
                     LLVYNSSNQIAGRIFSYNQSFRDIGNVTGPLMGAAISANYGFRAVFLVTAGVVLFNAV
                     YSWNSLRRRRIPQISN"
     gene            2550..2924
                     /locus_tag="NBI74_003532"
     CDS             2550..2924
                     /locus_tag="NBI74_003532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309456.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secY/secA suppressor protein"
                     /protein_id="HCD1933813.1"
                     /translation="MTMYATLEEAIDAAREEFLADNPGIDAENANVQQFNAQKYVLQD
                     GDIMWQVEFFADEGEEGECLPMLSGEAAQSVFDGDYDEIEIRQEWQDENTLHEWDEGE
                     FQLEPPLDTEEGRTAADEWDER"
     gene            complement(2925..3152)
                     /locus_tag="NBI74_003533"
     CDS             complement(2925..3152)
                     /locus_tag="NBI74_003533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HCD1933814.1"
                     /translation="MRLIVVSIMVTLLSGCGSIISRTIPGQGHGNQYYPGVQWDVRDS
                     AWRYVTILDLPFSLVFDTLLLPIDIHHGPYE"
     gene            complement(3324..5867)
                     /gene="mdoH"
                     /locus_tag="NBI74_003534"
     CDS             complement(3324..5867)
                     /gene="mdoH"
                     /locus_tag="NBI74_003534"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis glucosyltransferase MdoH"
                     /protein_id="HCD1933815.1"
                     /translation="MNKTTEYIDAMPIAASEKAALPKTDIRAVHQALDAEHRTWARED
                     DSPQGSVKARLEQAWPDSLADGQLIKDDEGRDQLKAMPEAKRSSMFPDPWRTNPVGRF
                     WDRLRGRDVTPRYLARLTKEEQESEQKWRTVGTIRRYILLILTLAQTVVATWYMKTIL
                     PYQGWALINPMDMVGQDVWVSFMQLLPYMLQTGILILFAVLFCWVSAGFWTALMGFLQ
                     LLIGRDKYSISASTVGDEPLNPEHRTALIMPICNEDVNRVFAGLRATWESVKATGNAK
                     HFDVYILSDSYNPDICVAEQKAWMELIAEVGGEGQIFYRRRRRRVKRKSGNIDDFCRR
                     WGSQYSYMVVLDADSVMTGDCLCGLVRLMEANPNAGIIQSSPKASGMDTLYARCQQFA
                     TRVYGPLFTAGLHFWQLGESHYWGHNAIIRVKPFIEHCALAPLPGEGSFAGSILSHDF
                     VEAALMRRAGWGVWIAYDLPGSYEELPPNLLDELKRDRRWCHGNLMNFRLFLVKGMHP
                     VHRAVFLTGVMSYLSAPLWFMFLALSTALQVVHALTEPQYFLQPRQLFPVWPQWRPEL
                     AIALFASTMVLLFLPKLLSILLIWCKGTKEYGGFWRVTLSLLLEVLFSVLLAPVRMLF
                     HTVFVVSAFLGWEVVWNSPQRDDDSTSWGEAFKRHGSQLLLGLVWAVGMAWLDLRFLF
                     WLAPIVFSLILSPFVSVISSRATVGLRTKRWKLFLIPEEYSPPQVLVDTDRFLEMNRQ
                     RSLDDGFMHAVFNPSFNALATAMATARHRASKVLEIARDRHVEQALNETPEKLNRDRR
                     LVLLSDPVTMARLHFRVWNSPERYSSWVSYYEGIKLNPLALRKPDAASQ"
     gene            complement(5860..7395)
                     /gene="mdoG"
                     /locus_tag="NBI74_003535"
     CDS             complement(5860..7395)
                     /gene="mdoG"
                     /locus_tag="NBI74_003535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoG"
                     /protein_id="HCD1933816.1"
                     /translation="MMKMRWLSAAVMLTLYTSSSWAFSIDDVAKQAQSLAGKGYEAPK
                     SNLPSVFRDMKYADYQQIQFNHDKAYWNNLKTPFKLEFYHQGMYFDTPVKINEVTATA
                     VKRIKYSPDYFTFGDVQHDKDTVKDLGFAGFKVLYPINSKDKNDEIVSMLGASYFRVI
                     GAGQVYGLSARGLAIDTALPSGEEFPRFKEFWIERPKPTDKRLTIYALLDSPRATGAY
                     KFVVMPGRDTVVDVQSKIYLRDKVGKLGVAPLTSMFLFGPNQPSPANNYRPELHDSNG
                     LSIHAGNGEWIWRPLNNPKHLAVSSFSMENPQGFGLLQRGRDFSRFEDLDDRYDLRPS
                     AWVTPKGEWGKGSVELVEIPTNDETNDNIVAYWTPDQLPEPGKEMNFKYTITFSRDED
                     KLHAPDNAWVQQTRRSTGDVKQSNLIRQPDGTIAFVVDFTGAEMKKLPEDTPVTAQTS
                     IGDNGEIVESTVRYNPVTKGWRLVMRVKVKDAKKTTEMRAALVNADQTLSETWSYQLP
                     ANE"
     gene            7789..8946
                     /gene="mdoC"
                     /locus_tag="NBI74_003536"
     CDS             7789..8946
                     /gene="mdoC"
                     /locus_tag="NBI74_003536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoC"
                     /protein_id="HCD1933817.1"
                     /translation="MNPVPAQREYFLDSIRAWLMLLGIPFHISLIYSSHTWHVNSAEP
                     SLWLTLFNDFIHSFRMQVFFVISGYFSYMLFLRYPLKKWWKVRVERVGIPMLTAIPLL
                     TLPQFIMLQYVKGKAESWPGLSLYDKYNTLAWELISHLWFLLVLVVMTTLCVWIFKRI
                     RNNLENSDKTNKKFSMVKLSVIFLCLGIGYAVIRRTIFIVYPPILSNGMFNFIVMQTL
                     FYLPFFILGALAFIFPHLKALFTTPSRGCTLAAALAFVAYLLNLRYGSGDAWMYETES
                     VITMVLGLWMVNVVFSFGHRLLNFQSARVTYFVNASLFIYLVHHPLTLFFGAYITPHI
                     TSNWLGFLCGLIFVVGIAIILYEIHLRIPLLKFLFSGKPVVKRENDKAPAR"
     gene            complement(8954..10435)
                     /gene="clsC"
                     /locus_tag="NBI74_003537"
     CDS             complement(8954..10435)
                     /gene="clsC"
                     /locus_tag="NBI74_003537"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsC"
                     /protein_id="HCD1933818.1"
                     /translation="MMKKTPTSTKDSLPNKEMNDLPRLASAVLPLCSQHPGQCGLFPL
                     EKSLDAFAARYRLAEMAEHTLDVQYYIWQDDMSGRLLFSALLAAAKRGVRVRLLLDDN
                     NTPGLDDILRLLDSHPRIEVRLFNPFSFRLLRPLGYITDFSRLNRRMHNKSFTVDGVV
                     TLVGGRNIGDAYFGAGEEPLFSDLDVMAIGPVVEDVADDFARYWYCKSVSPLQQVLDV
                     PEGEMADHIELPASWHNDVMTHRYLRKMESSPFIHHLVDGTLPLIWAKTRLLSDDPAK
                     GEGKAKRHSLLPQRLFDIMGSPSERIDIISSYFVPTRAGVAQLLRMVRKGVKIAILTN
                     SLAANDVAVVHAGYARWRKKLLRYGVELYELKPTREQSSTLHDRGITGNSGASLHAKT
                     FSIDGKTVFIGSFNFDPRSTLLNTEMGFVIESETLAQLIDKRFIQSQYDAAWQLRLDR
                     WGRINWVDRHAKKEIILKKEPATSFWKRVMVKLASILPVEWLL"
     gene            complement(10377..10910)
                     /gene="ymdB"
                     /locus_tag="NBI74_003538"
     CDS             complement(10377..10910)
                     /gene="ymdB"
                     /locus_tag="NBI74_003538"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HCD1933819.1"
                     /translation="MKTRIHVVQGDITKLAVDVIVNAANPSLMGGGGVDGAIHRAAGP
                     ALLDACLKVRQQQGDCPTGHAVITLAGDLPAKAVVHTVGPVWRGGEQNEDQLLQDAYL
                     NSLRLVAANSYTSVAFPAISTGVYGYPRAAAAEIAVKTVSEFITRHALPEQVYFVCYD
                     EENAHLYERLLTQQGDE"
     gene            complement(11005..11316)
                     /locus_tag="NBI74_003539"
     CDS             complement(11005..11316)
                     /locus_tag="NBI74_003539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCD1933820.1"
                     /translation="MFRPFLNSLMLGSLFFPFIAIAGSTAQGGVIHFYGQIVEPACDV
                     STQSSPVEMNCPQNGSVPRKTYSSKALMSGNVKNVQIASVKVQYLDKQKKLAVMNIEY
                     N"
     gene            complement(11437..11769)
                     /gene="csgC"
                     /locus_tag="NBI74_003540"
     CDS             complement(11437..11769)
                     /gene="csgC"
                     /locus_tag="NBI74_003540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli assembly protein CsgC"
                     /protein_id="HCD1933821.1"
                     /translation="MNALLLLAALSSQITFNTTQQGDMYTIIPEVTLTQSCLCRVQIL
                     SLREGSSGQSQTKQEKTLSLPANQPIALTKLSLNISPDDRVKIVVTVSDGQSLHLSQQ
                     WPPSSEKS"
     gene            complement(11829..12287)
                     /gene="csgA"
                     /locus_tag="NBI74_003541"
     CDS             complement(11829..12287)
                     /gene="csgA"
                     /locus_tag="NBI74_003541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curlin major subunit CsgA"
                     /protein_id="HCD1933822.1"
                     /translation="MKLLKVAAIAAIVFSGSALAGVVPQYGGGGGNHGGGGNNSGPNS
                     ELNIYQYGGGNSALALQADARNSDLTITQHGGGNGADVGQGSDDSSIDLTQRGFGNSA
                     TLDQWNGKDSTMTVKQFGGGNGAAVDQTASNSSVNVTQVGFGNNATAHQY"
     gene            complement(12328..12783)
                     /gene="csgB"
                     /locus_tag="NBI74_003542"
     CDS             complement(12328..12783)
                     /gene="csgB"
                     /locus_tag="NBI74_003542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curlin minor subunit CsgB"
                     /protein_id="HCD1933823.1"
                     /translation="MKNKLLFMMLTILGAPGIAAAAGYDLANSEYNFAVNELSKSSFN
                     QAAIIGQAGTNNSAQLRQGGSKLLAVVAQEGSSNRAKIDQTGDYNLAYIDQAGSANDA
                     SISQGAYGNTAMIIQKGSGNKANITQYGTQKTAIVVQRQSQMAIRVTQR"
BASE COUNT         3215 a         3335 c         3089 g         3309 t
ORIGIN      
        1 cattttacgc tacattttag cattaaaaat tatttgttat ttacaagcgc ggcaatttcg
       61 cccagtcttc agtcacaaat tttggttgcg gacgaaaaaa tgcgacaata catacaattg
      121 cccgtatagg ttgaaaaaca ggattgatat gacgaatcta cccaagttct ccaccgcact
      181 gcttcatccg cgttattggt taacctggtt gggtattggc gtactttggt tggtcgtgca
      241 attgccctac ccggttatct accgcctcgg ttgtggatta ggaaaactgg cgttacgttt
      301 tatgaaacga cgcgcaaaaa ttgtgcatcg caacctggaa ctgtgcttcc cggaaatgag
      361 cgaacaagaa cgccgtaaaa tggtggtgaa gaatttcgaa tccgttggca tgggcctgat
      421 ggaaaccggc atggcgtggt tctggccgga ccgccgaatc gcccgctgga cggaagtgat
      481 cggcatggaa cacattcgtg acgtgcaggc gcaaaaacgc ggcatcctgt tagttggcat
      541 ccattttctg acactggagc tgggtgcgcg gcagtttggt atgcaggaac cggggattgg
      601 cgtttaccgc ccgaacgata atccactgat tgactggcta caaacctggg gccgtttgcg
      661 ctcaaataaa tcgatgctcg accgcaaaga tttaaaaggc atgattaaag ccctgaaaaa
      721 aggcgaagtg gtctggtacg caccggatca tgattacggc ccgcgctcaa gcgttttcgt
      781 cccgttgttt gccgttgagc aggctgcgac cacgaccgga acctggatgc tggcacggat
      841 gtccggcgca tgtctggtgc ccttcgttcc acgccgtaag ccagatggca aagggtatca
      901 gttgattata ctgccgccag agtgttctcc gccgctggat gatgccgaaa ctaccgccgc
      961 gtggatgaac aaagtggtcg aaaaatgcat catgatggca ccagagcagt atatgtggtt
     1021 acaccgtcgc tttaaaacac gcccggaagg cgttccttca cgctattaaa tcttccatgc
     1081 tggatgcttc agaatggcat ccggcattac cacagaaaat ccccctgatt tagcgataaa
     1141 agctctctgg attgcgcccc ctggaagtcg ggcgcataat tagtgtgctt atcttttctt
     1201 cttatgttca ccgcgcctgg cgcaccaaca gcggattgct atgtcaccct gtgaaaatga
     1261 cacccctata aactggaaac gaaacctgat cgtcgcctgg ctaggctgtt ttcttaccgg
     1321 tgccgccttc agcctggtaa tgcccttctt acccctctac gttgagcagc ttggcgttac
     1381 cggccactcc gccctgaata tgtggtccgg tattgtcttc agcattacat ttttattttc
     1441 ggccatcgcc tcaccgtttt ggggtggact cgccgatcgt aaaggccgaa aactcatgct
     1501 attacgctct gctctcggca tgggcatcgt gatggtgttg atgggactgg cacaaaatat
     1561 ctggcagttt ttgatcctgc gggcgcttct tggtttactt ggcggatttg tccccaacgc
     1621 taatgctctt atcgccacac aagtaccgcg taataaaagc ggctgggcgc tgggtacgct
     1681 ctccacaggc ggcgttagcg gcgcgttgct cggcccaatg gctggcggtt tgctcgccga
     1741 tagctacggc ttacgtccgg tattctttat taccgccagt gtgctcatac tctgcttttt
     1801 cgtcaccctg ttttgcatca gagaaaaatt ccagccggtc agcaaaaaag agatgctgca
     1861 catgcgggaa gtggtgacat cacttaaaaa cccgaaactg gtactcagcc tgtttgtcac
     1921 cacgttaatc atccaggtgg cgacgggctc aattgccccc attctgacgc tgtatgtccg
     1981 cgaactggcg ggtaacgtca gtaacgtcgc ctttatcagt ggcatgatcg cctcggtgcc
     2041 aggcgtggcg gctctgctaa gtgcaccacg actcggcaaa cttggcgatc gaatcggacc
     2101 cgaaaagatc ctgattacgg cgctgatctt ttctgtactg ctgttgatcc caatgtctta
     2161 cgttcagacg ccattgcaac ttgggatttt acgttttttg ctcggtgccg ccgatggtgc
     2221 actactcccc gccgtacaga cactattagt ttataactcg agcaaccaga tcgccgggcg
     2281 tatcttcagc tataaccaat cgtttcgtga tattggcaac gttaccggac cattgatggg
     2341 agcagcgatt tcagcgaact acggttttag ggcggtattt ctcgtcaccg ctggcgtagt
     2401 gttattcaac gcagtctatt catggaacag tctacgtcgt cgtcgaatcc cccagatatc
     2461 gaactgattt ttcgcctttc atacttgcaa aagcggagaa tcagctatcc ttttccctga
     2521 aacctcatca actcaaaggg agaatcgtga tgaccatgta cgcaacgctt gaagaagcca
     2581 ttgacgctgc acgcgaagaa tttcttgcag acaaccccgg catcgacgcc gaaaacgcga
     2641 atgtgcaaca gttcaatgcc caaaaatacg ttttgcagga cggcgacatc atgtggcaag
     2701 ttgagttttt tgccgacgaa ggggaagaag gtgaatgttt acccatgctt agcggtgaag
     2761 ctgcgcaaag tgtctttgac ggcgactatg atgagataga gatccgccag gagtggcaag
     2821 atgagaatac attacatgaa tgggacgagg gggaatttca gcttgagcca ccgctggata
     2881 ccgaggaagg acgcacagca gctgatgagt gggatgaacg ttaatcactc atacgggcca
     2941 tgatgaatgt cgatcggcag cagtaaggta tcgaaaacca gggagaatgg cagatcaagg
     3001 atcgtgacgt aacgccaggc ggagtcacgc acgtcccatt gcacaccggg ataatattgg
     3061 ttgccatgcc cctgccccgg tatagtgcga ctaatgatgc tgccacagcc actcagcaag
     3121 gtcaccatta tgctcaccac aattaaacgc atttgatacc tcttccgtaa aaaataccgg
     3181 cataacgccg gtatttttat tggctcgttg tttttgccgg atgcggcgtg aacgccttgc
     3241 cagacccaca aaatcttgca aaatcaataa attgcaggaa cgatgtaggc ctgataagcg
     3301 tagcgcatca ggcaactacg tttttattgc gaagccgcat ccggtttacg caatgccagt
     3361 ggattgagct ttatcccttc gtaataactc acccatgaag aatatctctc cggggaattc
     3421 cagacgcgga aatgcagacg ggccatcgtc accggatcgc ttagcagcac caggcgacga
     3481 tcgcgattca gcttctctgg cgtctcgttc agcgcctgtt caacgtggcg gtcacgggcg
     3541 atttccagca ccttgctggc gcggtgacgc gcggtcgcca ttgcggttgc cagagcgtta
     3601 aatgacgggt taaacaccgc gtgcataaaa ccatcatcaa gggagcgttg acgattcatc
     3661 tcaaggaacc gatcggtatc aaccagcacc tgcggcggtg aatactcttc cgggatcagg
     3721 aacagtttcc agcgtttggt tcgcagacca acggtggcac ggctggaaat caccgaaaca
     3781 aacggtgaca ggatcaacga gaagacaatc ggtgccagcc agaacagaaa acgcagatcc
     3841 agccacgcca tcccaacagc ccacactaac cctaacagca gctgtgagcc gtggcgtttg
     3901 aacgcttccc cccaggaagt ggagtcatca tcacgctgcg gcgaattcca cactacttcc
     3961 cagccaagga acgcgctgac gacgaagacc gtatggaaca gcatgcgtac cggagccagc
     4021 agcacggaaa acagcacttc cagcagcaac gataatgtaa cgcgccagaa gccgccgtat
     4081 tctttcgttc ctttgcacca gataagcaaa atgctcaata gcttcggcag gaacaacagc
     4141 accatggtcg aagcaaaaag tgcaatcgcc agctcaggac gccactgcgg ccataccggg
     4201 aacaactgcc gtggttgcag gaagtattgc ggttcggtca acgcatgtac tacctgcaat
     4261 gcagtagaga gcgcgaggaa cataaaccac agcggagcgg agagataaga catcacgccc
     4321 gtcaggaaca ccgcacggtg aaccgggtgc atacccttca ccaggaacag acggaagttc
     4381 atcaggttac cgtggcacca gcggcggtca cgttttagct catcaagcaa gttaggcggc
     4441 aattcttcat aagaacccgg gagatcgtaa gcaatccaga ccccccaacc tgcacggcgc
     4501 atcaacgccg cttccacgaa gtcatgtgac aggattgaac cggcaaaaga accttcgccc
     4561 ggcagcggag ccagtgcaca gtgctcgata aacggtttca cgcggataat cgcgttatgc
     4621 ccccagtagt gcgactcgcc aagttgccag aagtgcaaac cggctgtaaa cagtggccca
     4681 tacacgcggg tcgcgaactg ctgacagcgc gcatacagcg tatccatgcc ggacgctttc
     4741 ggcgacgact gaatgatccc ggcgttcggg ttggcttcca tcaggcgcac caggccgcac
     4801 aaacaatcac cggtcattac cgagtcagca tccagcacca ccatgtagct gtactggctg
     4861 ccccagcgac ggcagaagtc atcgatatta ccgcttttac gcttcacgcg acggcggcgg
     4921 cggcgataga aaatctgacc ttcgccaccg acttcagcga taagctccat ccaggctttt
     4981 tgctctgcga cgcagatatc cgggttataa ctgtcactca gaatgtagac atcaaagtgt
     5041 ttggcattcc cggtggcttt tactgattcc cacgttgcac gcaagccagc aaaaacacgg
     5101 ttcacgtctt cgttacagat aggcatgatc aacgccgtgc gatgctccgg gtttaatggt
     5161 tcatcgccaa ctgttgacgc agatatactg tatttatcgc gaccaataag cagttgcagg
     5221 aagcccatca acgccgtcca gaacccggcg gacacccaac agaacagtac cgcaaagagg
     5281 atcaggatac cggtttgcag catataaggc agaagctgca taaaggaaac ccacacatcc
     5341 tgaccaacca tatccatagg attaatcagc gcccacccct gataaggaag aatggtcttc
     5401 atataccagg tcgcgacaac agtttgcgcg agcgtcagga tcaacagaat gtaacggcgg
     5461 atggtaccga cggtacgcca cttttgctca ctctcctgct cttctttggt caaacgagcc
     5521 agatagcgcg gcgtcacatc gcgtccacgc aggcgatccc agaaacggcc taccgggttg
     5581 gtacgccacg ggtcgggaaa catcgaggag cgttttgctt ctggcatcgc ctttagctga
     5641 tcgcgccctt cgtcgtcttt aattaactgt ccatcagcaa gtgaatctgg ccaggcttgt
     5701 tccagacgcg cctttaccga gccttgcggg gagtcatcct cccgcgccca ggtgcggtgt
     5761 tcggcatcca gcgcctgatg aacggcgcgg atatcagtct tcggcaatgc cgctttctcg
     5821 cttgcggcga tgggcattgc gtcaatgtac tcagttgtct tattcattgg caggtaactg
     5881 gtagctccag gtttcactca acgtctgatc ggcattcacc agcgcagcac gcatttcagt
     5941 ggttttcttg gcatctttca ctttcacacg catcaccaga cgccaacctt tggtaaccgg
     6001 gttatagcgc accgtgcttt caactatctc accattatca ccaatgctgg tttgcgctgt
     6061 gaccggggta tcctctggca gttttttcat ctcagcgccg gtaaaatcga ccacaaaggc
     6121 gatagtaccg tcaggctggc gaatcaggtt cgactgcttc acatcccccg ttgaacgacg
     6181 cgtttgttgc acccatgcgt tatctggcgc atgcagtttg tcttcatcac ggctgaaggt
     6241 gatggtgtat ttaaagttca tctctttacc cggctccggc agctgatccg gcgtccagta
     6301 agcgacgatg ttatcgttgg tttcatcgtt agttggaatt tccaccagct caacgctgcc
     6361 tttgccccac tcccctttcg gagtcaccca tgcgcttgga cgaagatcgt aacgatcatc
     6421 gagatcttca aagcgggaga aatcacgacc gcgctgcaac agaccgaagc cttgcgggtt
     6481 ttccatcgag aagctgctga cagctaaatg tttcgggtta ttcaacggac gccagatcca
     6541 ttcgccatta ccagcatgga tagacagacc gttagagtcg tgcaactccg gacgatagtt
     6601 atttgcaggc gacggttggt tcggcccaaa caggaacata ctggttaacg gtgcaacccc
     6661 cagtttgccg actttatcgc gcagatagat tttcgactgc acatccacaa ccgtgtcacg
     6721 tcctggcata actacgaatt tgtaagcacc tgtcgcgcgc ggcgagtcaa gcaatgcata
     6781 aatggttaaa cgtttatcag tcggttttgg acgctcgatc cagaactctt tgaagcgtgg
     6841 aaattcttca cccgatggca aggcggtatc aattgccagg ccgcgtgcag aaaggccata
     6901 aacctgacct gcaccaatca cgcggaaata gctggccccg agcatgctga cgatttcatc
     6961 gtttttatct ttgctgttga tcgggtaaag cactttgaaa ccggcaaaac caaggtcttt
     7021 taccgtgtct ttgtcatgct gaacatcgcc gaaagtgaaa taatccgggc tgtatttgat
     7081 tcgtttgact gcggtggcag tcacttcatt tattttgacc ggggtatcga agtacatacc
     7141 ctgatggtag aactcgagtt tgaatggggt cttcagattg ttccagtacg ctttgtcatg
     7201 attaaactgg atctgctgat agtccgcgta tttcatatcg cggaaaacgg agggcaagtt
     7261 gcttttgggc gcctcatagc ctttcccggc taaggattga gcttgctttg cgacatcatc
     7321 aatactgaaa gcccagcttg aagatgtata cagggttaac attactgcag cactcaacca
     7381 acgcattttc atcatttgta gtttatgttt cataattagt aagcacttcc ccctttgtgt
     7441 gcttatatcg atccgatcta ttttaatgga taacctgcca ttccgacaac agaatcccgg
     7501 tatcgttcag cacactggct catggattaa gcacctgatt gcaaccgttt tcactttccg
     7561 tgcttctcct aaagacagaa gcatcgactt cgtgtagggt ttgccagcaa atttatttat
     7621 caaccgtctt acagacgctt ccattctggt tagtggtagc gatgatgcta tgcataaaaa
     7681 ccttgaagcc tgcgcctggc gcggtcaggt cggtgtcact atggcaacaa gcctaaaggc
     7741 ataacccgac agggataaga cgaaaaatct gagatcagtt aaccatatat gaatccagta
     7801 cccgcgcaac gtgaatattt cctcgactcc atccgcgcct ggctgatgtt gttagggatc
     7861 ccttttcata tttctttaat ctattcgagc catacatggc atgtgaatag cgccgagccg
     7921 tcattgtggc tgaccctttt taatgacttc atccactcgt tccgcatgca ggtgtttttc
     7981 gttatatccg ggtacttttc ctacatgctt tttttacgct atcccttgaa aaaatggtgg
     8041 aaagtacgtg tcgaacgtgt aggtatcccg atgttaacag ccatcccctt actgacattg
     8101 ccgcaattta ttatgctgca atacgtcaaa ggcaaagcgg aaagttggcc tgggctgtca
     8161 ttgtatgaca aatataatac gttggcctgg gaattaatat cacacctgtg gtttttactg
     8221 gtgttagtgg tcatgacgac gctgtgcgta tggatattta aacgcatcag aaataattta
     8281 gaaaattctg ataaaacgaa taaaaaattc tcgatggtaa aattatcggt gatttttttg
     8341 tgcctcggca tcggttatgc ggtaataaga agaacgattt ttattgtgta tccacccatt
     8401 ctgagtaacg gcatgttcaa ttttattgtc atgcaaacgt tattttattt gccgttcttt
     8461 atcctcggcg cactggcttt cattttccct catcttaaag ccttgtttac cacgccttct
     8521 cgtggctgta cccttgcagc agcattggcg tttgtcgctt atttactcaa cctgcgctat
     8581 ggcagtggcg atgcctggat gtatgaaacc gagtcggtga tcaccatggt cctcggtctg
     8641 tggatggtga atgtggtctt ctcctttggc caccgtttgc ttaacttcca gtcagcgcgg
     8701 gtgacttatt ttgttaacgc atcgctgttt atctatctgg ttcaccaccc gttaacgctg
     8761 tttttcggcg catacattac accacacatc acctccaact ggcttggttt tctctgtggc
     8821 ctgatattcg tagtagggat tgcgataatt ctgtatgaaa ttcatttacg catcccatta
     8881 ttgaagtttt tgttctctgg taaaccggtt gttaagcgtg agaacgataa agcacctgcc
     8941 cgttaagccg cgtttacaat aaccattcca cgggcaatat cgacgccagt ttgaccataa
     9001 cccgcttcca gaaactggtg gcgggttctt ttttgagaat aatctctttc tttgcatgac
     9061 gatcaaccca gttgatccgt ccccacctgt ccagacggag ctgccaggcc gcatcatact
     9121 ggctctgaat aaagcgttta tcaattaact gtgccagcgt ttcgctctct atcacgaagc
     9181 ccatttcagt attgagcaat gttgaacgcg gatcgaaatt gaaagagcca ataaacaccg
     9241 ttttaccatc gatgctaaag gttttagcat gcaggctggc accggaatta ccggttatac
     9301 cgcgatcgtg taatgtacta ctttgttcac gcgtcggctt gagttcatat aattccaccc
     9361 catagcgaag caattttttg cgccagcgcg cgtatccggc atggacgaca gcaacatcgt
     9421 tagcggcaag agaattggtt aggatcgcaa tctttacccc ttttctcacc atccgtaaga
     9481 gttgcgccac acctgcacgt gtcggtacaa aataggaaga gataatatcg atgcgttcac
     9541 tgggtgagcc catgatatcg aacaggcgct gcggtaacag tgaatgccgt tttgccttgc
     9601 cctccccttt cgccggatca tcacttaata aacgtgtttt cgcccagata agcggcaacg
     9661 ttccatcaac cagatgatgt ataaacggac tggattccat tttgcgtaag taacgatgcg
     9721 tcatgacatc gttatgccag gaggcgggta actcgatgtg atccgccatt tcaccttccg
     9781 ggacatccag cacctgctgt aagggggaaa ccgatttgca ataccagtag cgggcgaaat
     9841 catcggcaac gtcctctacc acgggtccta ttgccatgac atctaaatcc gaaaaaagtg
     9901 gctcttcccc tgccccaaaa taggcatcac caatatttcg tcctcccacc agcgtcacca
     9961 cgccatcgac agtgaaactt ttattgtgca tacggcgatt gagacgggaa aagtcggtga
    10021 tataaccaag cggacgtagc aaacgaaacg agaaaggatt aaaaagccgg acttcaatgc
    10081 gtggatggct gtcaagcaag cgtaaaatgt catcaagtcc gggcgtattg ttgtcgtcca
    10141 gcaacaaacg gacgcgaacg ccacgctttg ctgcggctaa cagggcggaa aacagtaacc
    10201 gacccgacat atcgtcctgc cagatgtaat actgaacatc cagcgtatgc tctgccattt
    10261 cagccagacg ataccgggcg gcaaacgcat ccagactttt ctccagagga aaaagtccac
    10321 actgaccggg atgttgcgaa cacagtggca gcaccgcgct cgccagccga ggcaaatcat
    10381 tcatctcctt gttgggtaag gagtctttcg tagaggtggg cgttttcttc atcataacag
    10441 acaaagtata cctgttcggg taaagcgtga cgggtaataa attctgaaac ggtttttact
    10501 gcgatttcag ccgctgccgc acggggataa ccataaaccc cagtactgat tgcaggaaaa
    10561 gccactgacg tatagctgtt tgccgccacc agtcgtaggc tattgagata ggcatcctgc
    10621 aaaagctggt cttcgttttg ttcaccacca cgccagactg gcccgacggt gtgcactacg
    10681 gctttagcgg gaagatcgcc tgcaagcgta ataacggcat gccccgtagg gcaatcgccc
    10741 tgctgctgcc tgacttttaa acaggcatcc agcagggccg gacccgctgc gcgatgaatg
    10801 gccccatcga cgccgccgcc tcccattaat gacggattag ccgcattcac aatcacatca
    10861 acggccagtt tggtaatatc accctgcaca acatgaatac gcgttttcat aacgcctcct
    10921 tacaccttct tgtttcctaa gtgtacagca ggctttttag ttaaatcgca tttttctgct
    10981 tagcaacgtg agttgttcag aaacttagtt atattcgatg ttcattacag ccagcttttt
    11041 ctgtttatca agatactgca cttttactga ggctatttgc acgtttttaa cattgccgct
    11101 cattaaggct ttgctggaat aggttttacg cggaacagaa ccattctgtg ggcagttcat
    11161 ttctacgggt gatgactggg tgctgacgtc acatgccggt tccacaattt ggccataaaa
    11221 atggatcacg cccccttgcg cggtgcttcc tgcaatggca ataaaaggaa aaaacaaact
    11281 gccgagcata agagagttta aaaaaggtct gaacatttat tctatttcct caatgattag
    11341 tcatccttga gggttgtgtt atccatactt tcaggttggc tgattcgcag cagaccattc
    11401 tctccaggtt catcttatgc tcgatatttc aacaaattaa gacttttctg aagagggcgg
    11461 ccattgttgt gataaatgaa gtgactgtcc atcagaaaca gtaacaacta ttttcacccg
    11521 atcgtccggg gaaatattta aactcaactt cgtcaaagca atgggttgat tagcaggcaa
    11581 tgagagggtc ttttcttgct tcgtctgact ttgccctgaa ctgccttcgc gcagggacaa
    11641 tatttgtact ctgcacagac aagattgagt aagagtgact tcaggaataa tggtgtacat
    11701 atccccttgc tgggtcgtat taaaggttat ctgactggaa agtgccgcaa ggagtaataa
    11761 cgcattcata ttcttctccc gaaaaaaaaa cagggcttac gccctgtttc tttcatacag
    11821 atgatgtatt agtactgatg agcggtcgcg ttgttaccaa agccaacctg agtgacgtta
    11881 acgctggagt tagatgcagt ctggtcaaca gcagcaccgt taccgccacc gaactgttta
    11941 acagtcatag tagaatcttt accattccac tgatcaagag tagcgctgtt gccgaaacca
    12001 cgctgagtca gatcgattga gctgtcatca gaaccttggc caacatctgc accattaccg
    12061 ccgccgtgct gggtaatggt cagatcagag ttgcgggcgt cagcttgcag agcaagagca
    12121 gagttaccgc caccgtactg gtaaatattc agctctgaat tcgggccgct gttattaccg
    12181 ccaccaccgt ggttgccgcc accgccgccg tactgaggaa caacacctgc cagagcgcta
    12241 ccggagaata cgattgctgc aattgctgct acttttaaaa gtttcatgta aaacccccat
    12301 cggattgatt taaaagtcga atggaaatta acgttgtgtc acgcgaatag ccatttgcga
    12361 ctgtctctgc actacaattg ccgttttttg agtaccatac tgtgtaatat ttgctttatt
    12421 accagaacct ttctggataa tcatcgcagt attaccataa gcaccttgcg aaatactggc
    12481 atcgttggca ctgcccgcct gatcaatata tgcaaggtta taatctcctg tctggtcaat
    12541 ctttgcccgg ttgctactac cttcttgcgc aacaaccgcc aaaagttttg agcctccctg
    12601 ccgtaactga gcactattat tagtcccagc ttgaccaatt atggctgcct gattaaatga
    12661 agacttactc aattcattta ccgcaaagtt atattctgaa ttagctaaat cataacctgc
    12721 tgcggctgca atcccaggcg cacccagtat tgttaacatc ataaataaca atttgttttt
    12781 catgttgtca ccctggacct ggtcgtacat ttaagaaatt aaatcatttc aacttggttg
    12841 ttaacgcaac ccgtattttg ttaacgctgc gttacgatgg aaagtatgtc tgcggaaata
    12901 tttttaataa ctcacccgcg cgttgtattt ttcttttttt gttcatac
//
LOCUS       DAHHTG010000118        12882 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.119, whole genome shotgun sequence.
ACCESSION   DAHHTG010000118 DAHHTG010000000
VERSION     DAHHTG010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12882)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12882)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12882
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.119"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            156..1214
                     /gene="citC"
                     /locus_tag="NBI74_003543"
     CDS             156..1214
                     /gene="citC"
                     /locus_tag="NBI74_003543"
                     /EC_number="6.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415151.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[citrate (pro-3S)-lyase] ligase"
                     /protein_id="HCD1933824.1"
                     /translation="MFGNDIFTRVKRSENKKMAEIAQFLHENDLSVDTTVEVFITVTR
                     DEKLIACGGIAGNIIKCVAISESVRGEGLALTLATELINLAYERHSTHLFIYTKTEYE
                     ALFRQCGFSTLTSVPGVMVLMENSATRLKRYAESLKKFRHPGNKIGCIVMNANPFTNG
                     HRYLIQQAAAQCDWLHLFLVKEDSSRFPYEDRLDLVLKGTADIPRLTVHRGSEYIISR
                     ATFPCYFIKEQSVINHCYTEIDLKIFRQYLAPALGVTHRFVGTEPFCRVTAQYNQDMR
                     YWLETPTISAPPIELVEIERLRYQEMPISASRVRQLLAKNDLTAIAPLVPAVTLHYLQ
                     NLLEHSRQDAAARQKTPA"
     gene            1229..1525
                     /gene="citD"
                     /locus_tag="NBI74_003544"
     CDS             1229..1525
                     /gene="citD"
                     /locus_tag="NBI74_003544"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase acyl carrier protein"
                     /protein_id="HCD1933825.1"
                     /translation="MKINQPAVAGTLESGDVMIRIAPLDTQDIDLQINSSVEKQFGDA
                     IRTTILDVLARYNVRGVQLNVDDKGALDCILRARLEALLARASGIPALPWEDCQ"
     gene            1522..2430
                     /gene="citE"
                     /locus_tag="NBI74_003545"
     CDS             1522..2430
                     /gene="citE"
                     /locus_tag="NBI74_003545"
                     /EC_number="4.1.3.34"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415149.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (pro-3S)-lyase subunit beta"
                     /protein_id="HCD1933826.1"
                     /translation="MISASLQQRKTRTRRSMLFVPGANAAMVSNSFIYPADALMFDLE
                     DSVALREKDTARRMVYHALQHPLYRDIETIVRVNALDSEWGVNDLEAVVRGGADVVRL
                     PKTDTAQDVLDIEKEILRIEKACGREPGSTGLLAAIESPLGITRAVEIAHASERLIGI
                     ALGAEDYVRNLRTERSPEGTELLFARCSILQAARSAGIQAFDTVYSDANNEAGFLQEA
                     AHIKQLGFDGKSLINPRQIDLLHNLYAPTQKEVDHARRVVEAAEAAAREGLGVVSLNG
                     KMVDGPVIDRARLVLSRAELSGIREE"
     gene            2441..3973
                     /gene="citF"
                     /locus_tag="NBI74_003546"
     CDS             2441..3973
                     /gene="citF"
                     /locus_tag="NBI74_003546"
                     /EC_number="2.8.3.10"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase subunit alpha"
                     /protein_id="HCD1933827.1"
                     /translation="MTQKIEQSQRQERVAAWNRRAECDLAAFQNSPKQTYQAEKARDR
                     KLCADLEEAIRRSGLQDGMTVSFHHAFRGGDLTVNMVMDVIAKMGFKNLTLASSSLSD
                     CHAPLVEHIRQGVVTRIYTSGLRGPLAEEISRGLLAEPVQIHSHGGRVHLVQSGELNI
                     DVAFLGVPSCDEFGNANGYSGKACCGSLGYAMVDADNAKQVVMLTEELLPYPHNPASI
                     EQDQVDLIVKVDRVGDAAKIGAGATRMTTNPRELLIARSAADVIVNSGYFKEGFSMQT
                     GTGGASLAVTRFLEDKMRSRDIRADFALGGITATMVDLHEKGLIRKLLDVQSFDSQAA
                     QSLARNPNHIEISANQYANWGSKGASVDRLDVVVLSALEIDTQFNVNVLTGSDGVLRG
                     ASGGHCDTAIASALSIIVAPLVRGRIPTLVDNVLTCITPGSSVDILVTDHGIAVNPAR
                     PELAERLQEAGIKVVSIEWLRERARLLTGEPQPIEFTDRVVAVVRYRDGSVIDVVHQV
                     KE"
     gene            3977..4528
                     /gene="citX"
                     /locus_tag="NBI74_003547"
     CDS             3977..4528
                     /gene="citX"
                     /locus_tag="NBI74_003547"
                     /EC_number="2.7.7.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase holo-[acyl-carrier protein]
                     synthase"
                     /protein_id="HCD1933828.1"
                     /translation="MHLLPELASHHAVSIPELLVSRDERQARQHAWLKRHPVPLVSFT
                     VVAPGPIKDSEVTRRIFNHGVTALRALAAKQGWQIQEQAALVSASGPEGMLSIAAPAR
                     DLKLATIELEHSHPLGRLWDIDVLTPEGDILSRRDYSLPPRRCLLCEQSAAVCARGKT
                     HQLTDLLNRMEALLNDVDACNVN"
     gene            4503..5381
                     /gene="citG"
                     /locus_tag="NBI74_003548"
     CDS             4503..5381
                     /gene="citG"
                     /locus_tag="NBI74_003548"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase CitG"
                     /protein_id="HCD1933829.1"
                     /translation="MSMPATSTKTTKLATSLIDEYALLGWRAMLTEVNLSPKPGLVDR
                     INCGAHKDMALEDFHRSALAIQGWLPRFIEFGACSAEMAPEAVLNGLRPIGMACEGDM
                     FRATAGVNTHKGSIFSLGLLCAAIGRLLQLNQPVTPTTVCSTAASFCRGLTDRELRTN
                     NSRLTAGQRLYQQLGLTGARGEAEAGYPLVINHALPHYLTLLDQGLDPELALLDTLLL
                     LMATNGDTNVASRGGEGGLRWLQREAQTLLNNGGIRTPADLDYLRQFDRECIERNLSP
                     GGSADLLILTWFLAQI"
     gene            5432..6895
                     /gene="citT"
                     /locus_tag="NBI74_003549"
     CDS             5432..6895
                     /gene="citT"
                     /locus_tag="NBI74_003549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/succinate antiporter CitT"
                     /protein_id="HCD1933830.1"
                     /translation="MSLAKDNIWKLLAPLVVMGVMFLIPVPDGMPPQAWHYFAVFVAM
                     IVGMILEPIPATAISFIAVTICVIGSNYLLFDAKELADPAFNAQKQALKWGLAGFSST
                     TVWLVFGAFIFALGYEVSGLGRRIALFLVKFMGKRTLTLGYAIVIIDILLAPFTPSNT
                     ARTGGTVFPVIKNLPPLFKSFPNDPSARRIGGYLMWMMVISTSLSSSMFVTGAAPNVL
                     GLEFVSKIAGIQISWLQWFLCFLPVGVILLIIAPWLSYVLYKPEITHSEEVATWAGDE
                     LKTMGALTRREWTLIGLVLLSLGLWVFGSEVINATAVGLLAVSLMLALHVVPWKDITR
                     YNSAWNTLVNLATLVVMANGLTRSGFIDWFANTMSTHLEGFSPNATVIVLVLVFYFAH
                     YLFASLSAHTATMLPVILAVGKGIPGVPMEQLCILLVLSIGIMGCLTPYATGPGVIIY
                     GCGYVKSKDYWRLGAIFGVIYISMLLLVGWPILAMWN"
     gene            7009..7815
                     /gene="rna"
                     /locus_tag="NBI74_003550"
     CDS             7009..7815
                     /gene="rna"
                     /locus_tag="NBI74_003550"
                     /EC_number="4.6.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease I"
                     /protein_id="HCD1933831.1"
                     /translation="MKAFWRNAALLAVSLLPFSSANAVALQAKQYGDFDRYVLALSWQ
                     TGFCQSQHDRNRNERDECRLQTETTNKADFLTVHGLWPGLPKSVAARGVDERRWMRFG
                     CATRPIPNLPEARASRMCSSPETGLSLETAAKLSEVMPGAGGRSCLERYEYAKHGACF
                     GFDPDAYFGTMVRLNQEIKESEAGKFLADNYGKTVSRRDFDAAFAKSWGKENVKAVKL
                     TCQGNPAYLTEIQISIKADAINAPLSANSFLPQPHPGNCGKTFVIDKAGY"
     gene            8045..8455
                     /gene="rnk"
                     /locus_tag="NBI74_003551"
     CDS             8045..8455
                     /gene="rnk"
                     /locus_tag="NBI74_003551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside diphosphate kinase regulator"
                     /protein_id="HCD1933832.1"
                     /translation="MSRPTIIINDLDAERIDILLEQPAYAGLPIADALNAELDRAQMC
                     SPEEMPHDVVTMNSRVKFRNLSDGEVRVRTLVYPAKMTDSNTQLSVMAPVGAALLGLR
                     VGDSIHWELPGGVATHLEVLELEYQPEAAGDYLL"
     gene            8811..9239
                     /gene="uspG"
                     /locus_tag="NBI74_003552"
     CDS             8811..9239
                     /gene="uspG"
                     /locus_tag="NBI74_003552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspG"
                     /protein_id="HCD1933833.1"
                     /translation="MYKTIIMPVDVFEMELSDKAIRHAEFLAQDDGVIHLLHVLPGSA
                     SLSLHRFAADVRRFEEHLQHEAEERLQTMVSHFTIDPSRIKQHVRFGSVRDEVNELAK
                     ELDADVVVIGSRNPSISTHLLGSNASSVIRHANLPVLVVR"
     gene            complement(9360..10925)
                     /gene="ahpF"
                     /locus_tag="NBI74_003553"
     CDS             complement(9360..10925)
                     /gene="ahpF"
                     /locus_tag="NBI74_003553"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017455864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HCD1933834.1"
                     /translation="MLDTNMKTQLKAYLEKLTKPVELIATLDDSAKSAEIKELLAEIA
                     ELSDKVTFKEDNSLPVRKPSFLITNPGSNQGPRFAGSPLGHEFTSLVLALLWTGGHPS
                     KEAQSLLEQIRHIDGDFEFETYYSLSCHNCPDVVQALNLMSVLNPRIKHTAIDGGTFQ
                     NEITDRNVMGVPAVFVNGKEFGQGRMTLTEIVAKIDTGAEKRTAEELNKRDAYDVLIV
                     GSGPAGAAAAIYSARKGIRTGLMGERFGGQILDTVDIENYISVPKTEGQKLAGALKVH
                     VDEYDVDVIDSQSASKLIPAAVEGGLHQIETASGAVLKARSIIVATGAKWRNMNVPGE
                     DQYRTKGVTYCPHCDGPLFKGKRVAVIGGGNSGVEAAIDLAGIVEHVTLLEFAPEMKA
                     DQVLQDKLRSLKNVDIILNAQTTEVKGDGSKVVGLEYRDRVSGDIHNIELAGIFVQIG
                     LLPNTNWLEGAVERNRMGEIIIDAKCETNVKGVFAAGDCTTVPYKQIIIATGEGAKAS
                     LSAFDYLIRTKTA"
     gene            complement(11096..11659)
                     /gene="ahpC"
                     /locus_tag="NBI74_003554"
     CDS             complement(11096..11659)
                     /gene="ahpC"
                     /locus_tag="NBI74_003554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003858764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HCD1933835.1"
                     /translation="MSLINTKIKPFKNQAFKNGEFIEITEKDTEGRWSVFFFYPADFT
                     FVCPTELGDVADHYEELQKLGVDVYAVSTDTHFTHKAWHSSSETIAKIKYAMIGDPTG
                     ALTRNFDNMREDEGLADRATFVVDPQGIIQAIEVTAEGIGRDASDLLRKIKAAQYVAS
                     HPGEVCPAKWKEGEATLAPSLDLVGKI"
     gene            12031..12777
                     /gene="dsbG"
                     /locus_tag="NBI74_003555"
     CDS             12031..12777
                     /gene="dsbG"
                     /locus_tag="NBI74_003555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbG"
                     /protein_id="HCD1933836.1"
                     /translation="MLKKILLLALLPAIAFAEELPAPVKAIEKQGITIIKTFDAPGGM
                     KGYLGKYQDMGVTIYLTPDGKHAISGYMYNEKGENLSNTLIEKEIYAPAGREMWQRME
                     QSHWLLDGKKDAPVIVYVFADPFCPYCKQFWQQARPWVDSGKVQLRTLLVGVIKPESP
                     ATAAAILASKDPAKTWQQYEASGGKLKLSVPANVSTEQMKVLSDNEKLMDDLGANVTP
                     AIYYMSKENTLQQAVGLPDQKTLNIIMRNK"
BASE COUNT         3056 a         3394 c         3263 g         3169 t
ORIGIN      
        1 gaaatactcc ttaggatgta tagcgaaagg agaaaaagat atacctcgat cacccccctt
       61 tctcccaagt gaaaataaaa ggttatcaat ttgcaacatt gaacaacatt cgttgcaaat
      121 cgataacaac atgcaccttc aggatactat ttattatgtt cggcaatgat attttcaccc
      181 gcgtaaaacg ttcagaaaat aaaaaaatgg cggaaatcgc ccaattcctg catgaaaatg
      241 atttgagcgt tgacaccaca gtcgaagtat ttattaccgt aacccgcgat gaaaagctta
      301 tcgcgtgcgg tggaattgcc ggaaatatta ttaaatgcgt tgctatcagt gaatccgttc
      361 gcggtgaagg actggcgctg acattagcca ctgaactgat aaacctcgcc tatgagcggc
      421 acagcacgca tctgtttatt tataccaaaa ccgaatacga ggcgctgttc cgccagtgcg
      481 gtttttccac gctgaccagc gtacccggcg tgatggtgct gatggaaaac agcgccacgc
      541 gactgaaacg ctatgccgaa tcgctgaaaa aatttcgtca tccagggaac aaaattggct
      601 gcattgtgat gaacgccaat ccctttacga atggtcaccg ttatctgatt caacaggctg
      661 cggcacaatg cgactggttg catctgtttt tagtcaaaga agattcttca cgcttcccct
      721 atgaagaccg gctggatctg gtgttaaaag gcaccgccga tattccacgc ctgactgtgc
      781 atcgcggctc cgaatacatc atctcccgcg caacgttccc ttgctacttc attaaagaac
      841 agagcgtcat taaccattgc tacaccgaaa ttgatctgaa gattttccgt cagtacctcg
      901 ctcccgcgct gggtgtaact caccgctttg tcggtactga acccttttgt cgcgttaccg
      961 cccagtacaa ccaggatatg cgctactggc tggaaacgcc gactatctcc gcaccgccca
     1021 tcgaactagt tgaaattgag cggctgcgtt accaggagat gccgatatcc gcttcccggg
     1081 tacgtcaact gctggcaaaa aacgatctca cggctatcgc gccgctggtc cctgcagtca
     1141 cgctgcatta tttgcagaac ctgcttgagc actcccgcca ggacgcggca gctcgtcaaa
     1201 agacccccgc atgagaaaca ggtgaaaaat gaaaataaac cagcccgccg ttgcaggcac
     1261 ccttgagtct ggggatgtga tgatacgcat cgccccactc gatacgcagg atatcgacct
     1321 gcaaatcaac agcagcgttg agaaacagtt tggcgatgca attcgcacca ccattctgga
     1381 cgttctcgcc cgctacaacg tgcgcggcgt acagctgaat gtcgatgaca aaggcgcact
     1441 ggactgcatt ttacgtgcac gactggaagc cctgctggca cgcgccagcg gtatcccggc
     1501 tctgccatgg gaggattgcc aatgatttcc gcttcgctgc aacaacgtaa aactcgcacc
     1561 cgccgcagta tgttgtttgt gcctggtgct aatgccgcga tggtcagcaa ctccttcatc
     1621 tacccggctg atgccctgat gtttgacctc gaagactccg tagcattgcg tgaaaaagac
     1681 accgcccgcc gcatggttta ccacgcgctg caacatccgc tttatcgcga tattgaaacc
     1741 attgtgcgtg taaacgcgct ggattccgaa tggggtgtta acgacctgga agccgtcgtt
     1801 cgcggtggtg cggacgttgt gcgtctgccg aaaaccgata ccgctcagga tgttctggat
     1861 atcgaaaaag agatcctgcg tatcgaaaaa gcctgtggtc gtgaacccgg cagtaccggc
     1921 ctgctggcgg cgattgaatc tccgctgggc attacccgcg cagtggaaat cgctcacgct
     1981 tccgagcgtt tgatcggtat cgccctcggc gcagaagact atgtgcgcaa cctgcgtaca
     2041 gaacgctccc cggaaggaac tgaactgctg ttcgctcgct gctccatttt gcaggccgcg
     2101 cgctctgcgg gtattcaggc gttcgatacc gtctattccg acgctaacaa cgaagccgga
     2161 ttcctgcaag aagccgccca catcaaacag ctgggctttg acggcaaatc gctgatcaac
     2221 ccgcgtcaga ttgatctgct gcacaacctc tacgcaccga cccagaaaga agtggatcac
     2281 gcccgccgcg tcgtagaagc cgctgaagcc gccgctcgcg aaggcctcgg cgtggtttcc
     2341 ctgaacggca agatggtgga cggtccggtt atcgatcgcg cccgtctggt gctctcccgt
     2401 gcagaacttt ccggcatccg cgaagaataa ggcaatcaaa atgacgcaga aaattgaaca
     2461 atctcaacga caagaacggg tagcggcctg gaatcgtcgc gctgaatgcg atcttgccgc
     2521 tttccagaac tcgccaaagc aaacctacca ggctgaaaaa gcgcgcgatc gcaaactgtg
     2581 cgccgatctg gaagaagcga ttcgtcgctc tggtttacag gacggcatga cggtttcctt
     2641 ccatcacgct ttccgtggcg gtgacctgac cgtcaatatg gtgatggacg tcatcgcgaa
     2701 gatgggcttt aaaaacctga ctctggcctc cagctccctg agtgattgcc atgcgccgct
     2761 ggtagaacac attcgccagg gcgtggttac ccgcatttat acctctggcc tgcgcggtcc
     2821 actggcggaa gaaatctccc gtggtctgct ggcggaaccg gtgcaaatcc actctcacgg
     2881 cggacgtgtg catctggtac agagcggcga actgaatatc gatgtggctt tcctcggcgt
     2941 cccgtcctgt gatgaattcg gtaatgccaa cggctactcc ggtaaagcct gctgcggctc
     3001 cctcggctat gcaatggttg atgccgacaa cgcaaaacag gtcgtgatgc ttaccgaaga
     3061 actgctgcct tatccgcata atccggcaag cattgagcaa gatcaggttg atctgatcgt
     3121 caaagttgat cgcgttggcg atgctgcaaa aatcggcgct ggtgctaccc gtatgaccac
     3181 taacccgcgc gaactgctta ttgcccgtag cgctgcggat gtgattgtta actctggcta
     3241 cttcaaagaa ggtttctcca tgcagaccgg caccggcggc gcttcgctgg cggtaacccg
     3301 tttcctggaa gacaaaatgc gtagccgcga tattcgcgcc gacttcgccc tcggcggtat
     3361 taccgcgacg atggttgacc tgcacgaaaa aggtctgatc cgcaaactgc tggatgtgca
     3421 gagctttgac agccaagctg cgcaatcgct ggcccgtaac cccaatcaca tcgaaatcag
     3481 cgccaaccaa tacgctaact gggggtcgaa aggcgcatcg gttgatcgtc tcgacgtggt
     3541 ggtactgagt gcgctggaaa ttgacaccca gttcaacgtt aacgtgctga ccggctctga
     3601 cggcgtactg cgtggtgctt ccggtggtca ctgcgatacc gcgattgcct ctgcgctttc
     3661 catcatcgtc gcgccgctgg tacgcggtcg tattccgacg ctggtggata acgtgctgac
     3721 ctgcatcacc ccgggctcca gtgtcgatat tctggtcaca gaccacggca tcgctgttaa
     3781 cccggcacgt ccggagctgg cagaacgtct gcaggaagcg gggatcaaag tggtttctat
     3841 tgagtggctg cgtgaacgtg cgcgtctgct gaccggtgaa ccacagccga ttgaattcac
     3901 cgaccgcgtc gttgccgttg tgcgttaccg cgatggctcg gtgatcgatg ttgtgcatca
     3961 ggtgaaggaa taagccatgc acctgcttcc tgaactcgcc agccaccatg cggtgtcaat
     4021 tcccgagctg ctcgtcagcc gggatgaaag gcaagcacgg caacacgcct ggctcaagcg
     4081 ccatcctgtt ccactggtct cctttaccgt ggttgcgcca gggccgatta aagacagcga
     4141 ggtcacacgc cgaattttta atcatggcgt gacagccttg cgtgccttag ccgcaaaaca
     4201 gggctggcaa attcaggagc aggctgcact ggtttccgcc agcgggccgg agggcatgtt
     4261 gagcattgcc gccccggctc gcgacctcaa gctcgccacc attgagcttg aacatagtca
     4321 tcctctcggg cggttatggg atatcgatgt cctgacgccc gaaggcgaca ttctctcccg
     4381 ccgcgattat tcactgccgc ctcgccgctg cctgttgtgc gagcaaagcg cagctgtttg
     4441 cgcgcgtgga aaaacccatc aactgaccga tttactcaac cgcatggagg cgctgctaaa
     4501 cgatgtcgat gcctgcaacg tcaactaaaa ccacaaagct tgcgacgtca ttaattgatg
     4561 agtacgccct gctgggctgg cgcgccatgc tgactgaagt caacctgtca ccgaaaccag
     4621 gcctcgtgga tcgcattaac tgcggtgcgc acaaagatat ggcgctggaa gatttccacc
     4681 gcagcgcgct ggcgattcag ggctggttac cccgtttcat tgaatttggt gcctgtagtg
     4741 cagaaatggc accggaagcg gtactcaacg gattacgccc aattggtatg gcttgcgaag
     4801 gtgatatgtt ccgcgccact gcgggcgtaa acacgcataa aggaagcatt ttttctttag
     4861 ggctactgtg tgcggcaatt ggccgtttgc ttcagctcaa ccagccggta acgccaacaa
     4921 ccgtttgttc tacggcggca agtttctgcc gtggcctgac cgatcgcgaa ctgcgtacca
     4981 ataattcacg actgacggca ggtcaacggt tgtaccagca gcttggcctt accggcgcac
     5041 gcggtgaagc cgaagcaggt tatccactgg taatcaacca cgccttgccg cattacctca
     5101 ctctgctgga tcaggggtta gatcctgaac tggcactgct cgataccttg ctcctactga
     5161 tggctaccaa cggcgatacc aacgttgcat cgcgcggtgg cgaggggggc ctgcgctggc
     5221 tacagcgcga ggcgcaaaca ctattaaaca atgggggcat ccgaaccccc gccgatctcg
     5281 attatctccg gcagttcgac agggagtgta tcgaacgaaa tctcagtcca ggcggcagtg
     5341 ccgacctgct gattcttacc tggtttttag cacagattta attatttaag cacttgataa
     5401 atttggaaat attaattttc ggagaacccg tatgtcttta gcaaaagata atatatggaa
     5461 actattggcg cccctggtgg tgatgggtgt catgtttctt atccctgtac ctgacggtat
     5521 gccgccgcag gcgtggcatt actttgcagt gtttgtggca atgattgtcg gcatgatcct
     5581 cgagccaatt ccggcaacgg cgatcagttt tattgcggtt actatttgcg ttattggcag
     5641 taattacctg ctctttgatg ccaaagaatt agctgaccca gcgtttaatg cgcaaaaaca
     5701 ggcgctgaaa tggggtctgg ctggtttttc cagcaccacg gtatggctgg tatttggcgc
     5761 atttattttt gcattagggt atgaagtttc cgggttaggc cgtcgtattg cccttttcct
     5821 ggtgaaattc atggggaaac gcacgctgac gttgggttat gcgattgtca ttatcgacat
     5881 tctgctggca ccgtttacac cgtccaacac cgcgcgtacc gggggtacgg tttttccggt
     5941 cattaaaaac ctgccgccgc tgtttaaatc attcccgaac gatccgtccg cgcgtcgtat
     6001 tggcggctat ttgatgtgga tgatggtcat tagtaccagt ctgagttcgt ccatgtttgt
     6061 caccggtgcg gcaccaaacg tgctgggtct ggagttcgtc agcaaaattg ccggtatcca
     6121 gattagctgg ttgcagtggt tcctctgctt cctgccggtt ggggttatct tgcttatcat
     6181 tgcgccgtgg ctttcctacg tgctgtacaa accggaaatc acacacagtg aagaagtggc
     6241 aacctgggcg ggtgatgaac taaaaaccat gggtgcgctg acgcgcagag agtggacgct
     6301 gattggcctt gtattgctca gcttaggttt gtgggtattt ggcagtgaag tcattaatgc
     6361 tactgcggta ggtctgctgg cagtttcgct gatgctggcg ctgcacgttg tgccgtggaa
     6421 agacattact cgctataaca gcgcatggaa cacgctggtc aacctggcaa cgctggttgt
     6481 tatggcgaat ggcctgaccc gttctggctt tatcgactgg ttcgccaaca cgatgagtac
     6541 acacctggaa ggcttctcac caaacgcaac ggtgattgta ctggttctgg tgttctactt
     6601 tgcacactac ctgtttgcca gcctgtctgc gcacaccgca accatgctgc cggttattct
     6661 ggccgtcggt aaagggattc cgggcgtacc aatggaacaa ctgtgtatcc tgctggtgct
     6721 gtctatcggt atcatgggct gtctgacgcc ttatgcaacc ggtcctgggg tgattattta
     6781 cggctgtggc tatgtgaaat caaaagatta ctggcgtctt ggcgcaatct tcggggtgat
     6841 ttacatctct atgctgctgt tggttggctg gccgattctc gccatgtgga actaatacgt
     6901 tcgccaaacg ctgaataaat catcacgccc gccaggtaag cccacctggc gggctttttt
     6961 atgatttaat agataatatt cagtgtatgt atgaattcca cacccattat gaaagcattc
     7021 tggcgtaacg ccgcgttgct cgcggtttct ctgcttccct tctcttctgc caacgccgtg
     7081 gcgttgcagg caaaacagta tggcgatttt gatcgctatg tcctggccct ctcctggcaa
     7141 accggatttt gccagagtca acacgatcga aatcgtaacg aacgagatga atgtcgcctg
     7201 caaaccgaaa cgaccaacaa agctgatttt ttgaccgtac atggtctgtg gccaggattg
     7261 cctaaatcgg ttgctgcccg tggtgttgat gaacgccgct ggatgcgctt cggttgcgct
     7321 actcgcccaa tcccgaatct gccagaagca cgcgccagcc ggatgtgttc atcgccggaa
     7381 accgggttat cgctggaaac ggcagctaaa ctaagtgaag tcatgccagg agctggcgga
     7441 cgttcctgcc tggaacgcta cgaatatgcc aaacacggtg cctgctttgg ttttgatcca
     7501 gacgcatact tcggcacgat ggtacgcctg aatcaagaaa ttaaagagag cgaagccgga
     7561 aaattccttg cggataacta cggtaaaaca gtgagccgcc gtgactttga tgccgccttt
     7621 gccaaaagct ggggaaaaga gaacgtgaaa gcagttaagc taacgtgcca gggtaaccct
     7681 gcgtatttga ctgaaattca gatctcgatc aaagctgacg ccatcaacgc tccgctttct
     7741 gccaactcat tcttgccaca accacaccca ggcaactgtg gcaaaacctt tgtgattgat
     7801 aaagcaggtt attaatcctc tgatttacaa aacacgctaa ttcgtcttcc ataaaccatg
     7861 acgattaaga tttatacata atgcgctaca ttgaggaatt ccctgaatgt gacgcaaatc
     7921 acttcaaaga gtgagcaaca gtcagtatca tcatgtgagt taaaccctcg ccgcctgacg
     7981 gtgagggttt tcttttgggt atgtttatct gcgacactcg cagtaccgac gacatggagt
     8041 aaaaatgtcc agaccaacta tcatcattaa cgacctggat gccgaacgca tcgatattct
     8101 gctggagcaa cccgcctatg ctggtttgcc aatcgccgac gcgttaaacg cagagttgga
     8161 tcgcgcccaa atgtgttcgc cagaagagat gccacacgac gtggtgacaa tgaacagccg
     8221 ggttaaattc cgcaatctta gcgatggcga agtgcgtgtg cgcacgctgg tgtatccggc
     8281 aaaaatgacc gatagcaata ctcagctttc cgttatggct ccggtaggtg ccgcactgct
     8341 ggggctgcgc gttggcgatt ccattcactg ggaacttccg ggcggcgttg caacccacct
     8401 tgaagtgctg gaactcgaat accagccaga agctgctggc gactacctgc tttaatccca
     8461 tctgcacaag cgataactcg ctttgcccag aggatgcact cgcatcctct ttccgctcaa
     8521 atatcaacca gatttatcat tttgctctta agcgcagcat tctaatctga actggttatt
     8581 catctgcgga acctcatatg agcgaagctc tcggtatcac tgcattttat atattcatag
     8641 cagcaattat tgttgctgca gtgctatacc ttgaacaacg ctggtagcgc ctggttttat
     8701 ttattttcca gctcaatagc aaattcagat caaatcagcc tcgcaaatta tagattcata
     8761 accaataatg tgctgttatg tatacatgca aaaacaagga gagaatgctt atgtacaaaa
     8821 caatcatcat gccagtggat gttttcgaaa tggaattgag cgacaaagcg attcgccacg
     8881 ctgaattcct cgcccaggat gacggagtta ttcatctact tcacgtacta ccggggtcag
     8941 ccagcctgag cctgcaccgt tttgccgctg atgtacgtcg ttttgaagag catctgcaac
     9001 atgaagcaga agaacgtttg caaacgatgg tcagccactt caccatcgat ccttcccgca
     9061 ttaagcaaca tgtccgtttt ggtagtgtgc gggatgaagt taacgagtta gcgaaagaac
     9121 ttgatgcaga tgtagtagtg attggttcgc gtaacccatc aatttcaact catctgttgg
     9181 gttctaacgc ctcgagcgta atccgccacg ccaatttgcc ggtgctggtt gtgcgttaat
     9241 tgccagaata agtctcccgc cctgcccgga gccatccctg cagggctttt taatgccata
     9301 aaaaagccgc caggtttgac cccggcggct aagcaattac aggtgaatct tacttcttct
     9361 tatgcagttt tggtgcgaat caggtagtca aaagcactca gagaggcttt ggcaccttcg
     9421 ccagtggcga tgatgatctg cttgtacgga accgtcgtac agtcacccgc tgcgaacacg
     9481 cctttgacgt tggtttcgca tttcgcatca atgataatct cgcccatgcg attacgttcg
     9541 actgcgcctt ccagccagtt ggtgttcggc agcagaccaa tctggacgaa aataccggcc
     9601 agttcgatgt tgtgaatatc gccgctgaca cgatcgcgat attccagacc tacgactttg
     9661 ctaccgtcgc ctttcacttc cgtggtttgc gcattcagaa taatgtcgac gtttttcagg
     9721 ctgcgcagtt tgtcctgcag aacctggtcg gctttcattt ctggcgcaaa ttccagcagc
     9781 gttacgtgct caacgatacc cgccaggtca attgccgctt ccacgccgga gttaccgccg
     9841 ccgataaccg ctacgcgttt gcctttaaac agcgggccgt cgcagtgcgg gcagtaggtc
     9901 acgcctttgg tgcgatactg atcttcgcca ggaacgttca tgttgcgcca ttttgcacca
     9961 gtcgccacga taatgctgcg tgctttcagt accgcgccag aagctgtttc aatctgatgc
    10021 aggccgcctt caaccgctgc cgggatcagt ttgctcgcgc tctggctgtc gatcacatca
    10081 acgtcgtatt catcaacatg aactttcagc gcacctgcca gtttctggcc ttcggtcttc
    10141 ggtacagaaa tgtagttttc gatatcaacg gtatcgagga tctgaccacc aaaacgttcg
    10201 cccatcagac cggtacgaat gcctttacgt gcggagtaaa ttgctgccgc tgcacccgca
    10261 gggccggaac caacgattaa tacgtcataa gcatcacgct tgttcagctc ttctgccgta
    10321 cgtttttctg caccagtatc aattttggca acgatttcag tcaacgtcat gcggccctga
    10381 ccaaactctt tcccgtttac gaacactgcc ggaacgccca tcacgttgcg atcggtgatt
    10441 tcgttctgga aggtgccgcc gtcaattgca gtgtgcttga tgcgcgggtt cagtacgctc
    10501 atcaggttca gcgcctgcac cacgtccggg cagttgtggc aagagagcga gtaataggtt
    10561 tcgaattcaa aatcaccgtc aatatggcga atctgctcca gcagagactg cgcttctttc
    10621 gacggatgac caccggtcca caacaacgcc agtaccagcg aggtgaactc gtggcctagc
    10681 ggggagcctg caaaacgtgg tccctggttg gaacctgggt tggtgatcag gaaagacggc
    10741 ttacgcaccg gcaagctgtt atcttcttta aaggtgactt tgtctgacag ttctgcgatt
    10801 tcagccaaca gttccttgat ttctgccgat ttagcgctgt catccagcgt ggcaattaac
    10861 tcaacaggct tggtcaattt ctcaaggtaa gccttgagtt gagttttcat atttgtgtcg
    10921 agcatgttta tctcctgggc tttaaacatc atcatgcaag cggcctctgc ggctagctgg
    10981 atgcagcttg catcatggtg cggggaagaa aatcgggtgc cgcagcaccc gaagaattag
    11041 gtgaatttcc gaagtatctc gcgccggagt tagtagcgcg aaagacaaag gaaatttaga
    11101 ttttaccaac caggtccaga gacggagcca gagttgcttc accttctttc catttagccg
    11161 ggcaaacttc acctgggtga gaagctacgt actgtgctgc tttgatttta cgcagcaggt
    11221 cagacgcgtc acggccaatg ccttcagcgg taacttcgat tgcctggatg ataccctgcg
    11281 ggtcaacaac gaaggtcgca cggtcagcca gaccttcatc ttcacgcatg ttgtcgaagt
    11341 tacgggtcag ggcgccagtc gggtcgccga tcatcgcata tttgatttta gcgatggttt
    11401 cagagctgct gtgccatgct ttgtgggtga agtgagtatc ggtagatact gcgtatacgt
    11461 ctacgcccag tttctgcagt tcttcgtaat ggtcagcaac gtcacccagt tcggtcgggc
    11521 atacgaaagt aaagtcagcc gggtagaaga agaagacgct ccagcggcct tcggtatctt
    11581 tttcggtgat ttcgatgaat tcgccgtttt tgaatgcctg gtttttaaaa ggtttaattt
    11641 tggtgttaat caaggacatc tatacttcct ccgtgttttc gatgagatgt aaggtaacca
    11701 atttttgccg attcggctaa tgcgtttcca ttatcaaatc gataagtttt accttacaac
    11761 cttcgtaaga caactttgtg aactttgcac cgccagcggc gataaaaagt aaaatggccc
    11821 cctttccagg tgaccgtgta atctgaacta ccctttggta tcttcagcgc ctgcctgcag
    11881 gctgaggtgt tgcccttaca aatgcaacaa cgacatggat tacaacaccc tcataaacaa
    11941 agggcaatca cctgatctaa gctcttacct atgacagtga taggttatgc cttttactcg
    12001 acttttgcac tgactgaaaa ggacaaatta atgttaaaaa agatactttt actggccctg
    12061 cttcctgcaa tcgccttcgc agaggaactt cctgccccag taaaagcgat tgaaaaacag
    12121 ggtattacaa tcatcaaaac gttcgatgcc cctggaggaa tgaaaggtta tctcggtaag
    12181 tatcaggata tgggcgtcac catctacctg actccagatg gtaagcacgc tatctctggt
    12241 tacatgtaca acgaaaaagg tgaaaacctg agtaacacgc ttatcgaaaa agaaatttac
    12301 gcacccgccg gacgtgaaat gtggcaacgg atggaacaat cccactggct cctcgacggt
    12361 aaaaaagacg cgccggtcat tgtctacgtc ttcgccgatc cgttctgccc atattgtaaa
    12421 cagttctggc agcaggctcg tccgtgggta gattctggca aagtgcaatt gagaacattg
    12481 ttggttgggg ttatcaagcc agaaagcccg gcgacagcag cggcaattct tgcctccaaa
    12541 gatcccgcaa aaacctggca acaatatgaa gcctctggtg gcaagcttaa gctaagcgtg
    12601 cctgcaaacg taagcacaga gcaaatgaaa gtgttaagcg acaacgagaa actcatggac
    12661 gatctggggg caaatgtcac accagctatc tattacatga gtaaggaaaa taccctgcaa
    12721 caagccgtcg gattacccga tcagaaaacg cttaatatca ttatgaggaa taaataaaag
    12781 ataattcatg gatggttgaa atacatcatt gcttttcaac catcctaaaa acgcatgaat
    12841 tgctcgtaac ccacctgaca gctatgctat tttagaaaat ct
//
LOCUS       DAHHTG010000119        12811 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.120, whole genome shotgun sequence.
ACCESSION   DAHHTG010000119 DAHHTG010000000
VERSION     DAHHTG010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12811)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12811)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12811
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.120"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            <1..108
                     /locus_tag="NBI74_003556"
     CDS             <1..108
                     /locus_tag="NBI74_003556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter"
                     /protein_id="HCD1933837.1"
                     /translation="SISANEIFRMAAIAFILLTVLVWFAKPPFTAKGVG"
     gene            complement(216..1544)
                     /gene="dsdA"
                     /locus_tag="NBI74_003557"
     CDS             complement(216..1544)
                     /gene="dsdA"
                     /locus_tag="NBI74_003557"
                     /EC_number="4.3.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine ammonia-lyase"
                     /protein_id="HCD1933838.1"
                     /translation="MENAKMNSLIAQYPLVKDLVALKETTWFNPGTTSLAEGLPYVGL
                     TEQDVQDAHARLSRFAPYLAKAFPETAATGGIIESELVAIPAMQKRLEKEYQQPISGQ
                     LLLKKDSHLPISGSIKARGGIYEVLAHAEKLALEAGLLTLEDDYSKLLSPEFKQFFSQ
                     YSIAVGSTGNLGLSIGIMSARIGFKVTVHMSADARAWKKAKLRSHGVTVVEYEQDYGV
                     AVEEGRKASQSDPNCFFIDDENSRTLFLGYSVAGQRLKAQFAQQGRIVDADNPLFVYL
                     PCGVGGGPGGVAFGLKLAFGDHVHCFFAEPTHSPCMLLGVHTGLHDQISVQDIGIDNL
                     TAADGLAVGRASGFIGRAMERLLDGFYTLSDQTMYDMLGWLAQEEGIRLEPSALAGMA
                     GPQRVCASVSYQQMHGFSAEQLRNATHLVWATGGGMVPEEEMNQYLAKGR"
     gene            complement(1562..2899)
                     /gene="dsdX"
                     /locus_tag="NBI74_003558"
     CDS             complement(1562..2899)
                     /gene="dsdX"
                     /locus_tag="NBI74_003558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine transporter DsdX"
                     /protein_id="HCD1933839.1"
                     /translation="MHSQIWVVSTLLISIVLIVLTIVKFKFHPFLALLLASFFVGTMM
                     GMGPLDMVNAIESGIGGTLGFLAAVIGLGTILGKMMEVSGAAERIGLTLQRCRWLSAD
                     VIMVLVGLICGITLFVEVGVVLLIPLAFSIAKKTNTSLLKLAIPLCTALMAVHCVVPP
                     HPAALYVANKLGADIGSVIVYGLLVGLMASLIGGPLFLKFLGQRLPFKPVPTEFADLK
                     VRDEKTLPSLGATLFTILLPIALMLVKTIAELNMARESGLYTLLEFIGNPITAMFIAV
                     FVAYYVLGIRQHMSMGTMLTHTENGFGSIANILLIIGAGGAFNAILKSSSLADTLAVI
                     LSNMHMHPILLAWLVALILHAAVGSATVAMMGATAIVAPMLPLYPDISPEIIAIAIGS
                     GAIGCTIVTDSLFWLVKQYCGATLNETFKYYTTATFIASVIALAGTFLLSFII"
     gene            3117..4052
                     /gene="dsdC"
                     /locus_tag="NBI74_003559"
     CDS             3117..4052
                     /gene="dsdC"
                     /locus_tag="NBI74_003559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator DsdC"
                     /protein_id="HCD1933840.1"
                     /translation="MEPLREIRNRLLNGWQLSKLHTFEVAARHQSFALAAEELSLSPS
                     AVSHRINQLEEELGIQLFVRSHRKVELTHEGKRVYWALKSSLDTLNQEILDIKNQELS
                     GTLTLYSRPSIAQCWLVPALGDFTRRYPSISLTVLTGNDNVNLQRAGIDLAIYFDDAP
                     SAQLTHNFLMDEEILPVCSPEYAHRHDLTNTVINLRHCTLLHDRQAWSNDSGTDEWHS
                     WAQHYAVNLPTSSGIGFDRSDLAVIAAMNHVGVAMGRKRLVQKRLASGELVAPFGDMT
                     VKCHQHYYITTLPGRQWPKIEAFITWLREQVKTTS"
     gene            complement(4152..4226)
                     /locus_tag="NBI74_003560"
     tRNA            complement(4152..4226)
                     /locus_tag="NBI74_003560"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4191..4193),aa:Arg,seq:cct)
     gene            complement(4302..5234)
                     /locus_tag="NBI74_003561"
     CDS             complement(4302..5234)
                     /locus_tag="NBI74_003561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HCD1933841.1"
                     /translation="MDNDKIDQHSDEIEVESEEKERGKKIEIDEDRLPSRAMAIHEHI
                     RQDGEKELERDAMALLWSAIAAGLSMGASLLAKGIFHVELEGVPGSFLLENLGYTFGF
                     IIVIMARQQLFTENTVTAVLPVMQKPTMSNVGLLMRLWGIVLLGNILGTGIAAWAFEY
                     MPIFNEETRDAFVKIGMDVMKNTPSEMFANAIISGWLIATMVWMFPAAGAAKIVVIIL
                     MTWLIALGDTTHIVVGSVEILYLVFNGTLHWSDFIWPFALPTLAGNICGGTFIFALMS
                     HAQIRNDMSNKRKAEARQKAERAENIKKNDKNPA"
     gene            5528..6283
                     /gene="mlaA"
                     /locus_tag="NBI74_003562"
     CDS             5528..6283
                     /gene="mlaA"
                     /locus_tag="NBI74_003562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding lipoprotein MlaA"
                     /protein_id="HCD1933842.1"
                     /translation="MKLRLSALALGTTLLVGCASSGTDQQGRSDPLEGFNRTMYNFNF
                     NVLDPYIVRPVAVAWRDYVPQPARNGLSNFTGNLEEPAVMVNYFLQGDPYQGMVHFTR
                     FFLNTILGMGGFIDVAGMANPKLQRTEPHRFGSTLGHYGVGYGPYVQLPFYGSFTLRD
                     DGGDMADSLYPVLSWLTWPMSVGKWTLEGIETRAQLLDSDGLLRQSSDPYIMVREAYF
                     QRHDFIANGGELKPQENPNAQAIQDDLKDIDSE"
     gene            complement(6345..7685)
                     /gene="fadL"
                     /locus_tag="NBI74_003563"
     CDS             complement(6345..7685)
                     /gene="fadL"
                     /locus_tag="NBI74_003563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid transporter FadL"
                     /protein_id="HCD1933843.1"
                     /translation="MSQKTLFTKSALAVAVALISTQAWSAGFQLNEFSSSGLGRAYSG
                     EGAIADDAGNVSRNPALITMFDRPTFSAGAVYIDPDVDITGHSNLTGQSANAKNIAPT
                     AWVPNLHFVAPINDQFGWGASITSNYGLATEFTDNYAAGIYGGKTDLQTINLNLSGAY
                     RLNNSWSFGLGFDAVYAKAKIERYAGSLGPLLSQQLIAQGVPASLTNGINTPDSQIAH
                     LKGDEWGFGWNAGILYELDKDNRWGLTYRSEVKIDFDGDYKSSINPNLNNILGNMGLP
                     YGTMGATQNGSLTLNLPEMWEISGYNRVAPQWAIHYSMAYTSWSQFQELKAKGDSGQT
                     LFYKDEGFRDAYRIALGTTYYYDKNWTFRTGIAYDDSPVPADKRSISIPDQDRLWLSA
                     GLTYAFNEDASIDVGASYMHGQKVKFTEGEGAAAYSFESEGKAWLFGTNFNYAF"
     gene            8057..8341
                     /locus_tag="NBI74_003564"
     CDS             8057..8341
                     /locus_tag="NBI74_003564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcZ/YiiS family protein"
                     /protein_id="HCD1933844.1"
                     /translation="MSKCSADETPVCCCMDVGTIMDNSDCTASYSRVFANRAEAEQTL
                     AALTEKARSVESEPCKITPTFTEESDGVRLDIDFTFACEAEMLIFQLGLR"
     gene            8522..9832
                     /gene="fadI"
                     /locus_tag="NBI74_003565"
     CDS             8522..9832
                     /gene="fadI"
                     /locus_tag="NBI74_003565"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadI"
                     /protein_id="HCD1933845.1"
                     /translation="MGQVLPLVTRQGDRIAIVSGLRTPFARQATAFHGIPAVDLGKMV
                     VGELLARTEIPAEVIEQLVFGQVVQMPEAPNIAREIVLGTGMNVHTDAYSVSRACATS
                     FQAVANVAESLMAGTIRAGIAGGADSSSVLPIGVSKKLARVLVDVNKARTMSQRLKLF
                     SRLRLRDLMPVPPAVAEYSTGLRMGDTAEQMAKTYGITREQQDALAHRSHQRAAQAWS
                     EGKLKEEVMTAFIPPYKQPLVEDNNIRGNSSLADYAKLRPAFDRKHGTVTAANSTPLT
                     DGAAAVILMTESRAKELGLVPLGYLRSYAFTAIDVWQDMLLGPAWSTPLALERAGLTM
                     GDLTLIDMHEAFAAQTLANIQLLGSERFARDVLGRAHATGEVDESKFNVLGGSIAYGH
                     PFAATGARMITQTLHELRRRGGGFGLVTACAAGGLGAAMVLEAE"
     gene            9832..11976
                     /gene="fadJ"
                     /locus_tag="NBI74_003566"
     CDS             9832..11976
                     /gene="fadJ"
                     /locus_tag="NBI74_003566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadJ"
                     /protein_id="HCD1933846.1"
                     /translation="MEMASAFTLNVRLDNIAIITIDVPGEKMNTLKAEFASQVRAIIK
                     QLRENKELRGVVFVSAKPDNFIAGADINMIGNCKTAQEAEVLARQGQQLMAEIHALPI
                     PVIAAIHGACLGGGLELALACHGRVCTDDPKTVLGLPEVQLGLLPGSGGTQRLPRLIG
                     VSTALEMILTGKQLRAKQAVKLGLVDDVVPHSILLEAAVELAKQDRPSSRPLPVRERI
                     LAGPLGRALLFKMVGKKTEHKTQGNYPATERILEVVETGLAQGTSSGYDAEARAFGEL
                     AMTPQSQALRNIFFASTDVKKDPGSDAPPAPLNSVGILGGGLMGGGIAYVTACKAGLP
                     VRIKDINPQGINHALKFSWDQLEGKVRRRHLKASERDKQLALISGTTDYCGFAHRDLI
                     IEAVFENLELKQQMVAEVEQNCATHTIFASNTSSLPIGDIAAHATRPEQVIGLHFFSP
                     VEKMPLVEIIPHASTSAQTIATTVKLAKKQGKMPIVVRDKAGFYVNRILAPYINEAIR
                     MLTEGERIEHIDAALVKFGFPVGPIQLLDEVGIDTGTKIMPVLEAAYGERFSASANVV
                     SSILNDDRKGRKNGRGFYLYGQKGRKSKKQVDPAIYPLIGVQGQGRLSAPQVAERCVM
                     LMLNEAVRCLDEQVIRSVRDGDIGAVFGIGFPPFLGGPFRYIDSLGAGEVVAIMQRLA
                     TQYGSRFTPCDRLVEMSERGESFWKTTATDLQ"
     gene            12179..12664
                     /gene="sixA"
                     /locus_tag="NBI74_003567"
     CDS             12179..12664
                     /gene="sixA"
                     /locus_tag="NBI74_003567"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohistidine phosphatase SixA"
                     /protein_id="HCD1933847.1"
                     /translation="MQVFIMRHGDAALDAASDSVRPLTTHGCDESRLMANWLKGQKVE
                     IERVLVSPFLRAEQTLEEVGDCLNLPSSAEVLPELTPCGDVGLVGAYLQALTNEGVAS
                     VLVISHLPLVGYLVAELCPGETPPMFTTSAIASVTLDESGKGQFNWQMSPCNLKMAKA
                     I"
BASE COUNT         3253 a         3106 c         3241 g         3211 t
ORIGIN      
        1 tctatttctg caaatgagat tttccgtatg gcggctattg cttttatctt acttacggtt
       61 ttggtttggt ttgcgaaacc gccgtttaca gcgaaaggcg ttgggtgatg gaaaaagagg
      121 aggctatagc aggtaaaacg gcagaatctt aagtctgcgt catcggcagc atcgctcacc
      181 cagggaaagg atggtgatgc tgcgttgaaa cgttattaac ggccttttgc cagatattga
      241 ttcatctctt cttccggcac cattccacct cccgtcgccc acaccagatg agtggcatta
      301 cgcagttgtt ctgcgctgaa accgtgcatc tgttggtaac ttactgatgc gcacacgcgc
      361 tgaggtccgg ccatacccgc cagtgccgaa ggttcaagac gaataccttc ttcctgcgcc
      421 agccagccaa gcatgtcata catggtttga tcgctaaggg tatagaagcc atccagcaga
      481 cgctccatcg cccgcccgat aaagcccgat gcgcgaccaa ccgcaaggcc atcggcagcg
      541 gtaaggttgt cgatgccaat atcctgaaca gaaatctgat cgtgtaatcc ggtatggacg
      601 cctaataaca tacacgggga gtgtgttggt tcggcaaaaa agcagtgaac atgatcgcca
      661 aacgccagtt taagtccgaa tgcgacgcca ccaggaccac cgccaacacc acacggcaga
      721 tagacaaata gagggttatc agcatcgacg atacggcctt gctgggcaaa ttgcgcttta
      781 agacgctggc cagcgacgga atacccaagg aacaacgtgc gggaattctc gtcatcaata
      841 aagaaacagt tcgggtcaga ctgcgatgct ttacgtcctt cctcgacagc aacaccataa
      901 tcttgctcat attccacgac cgtaacgcca tggctgcgca gtttcgcttt tttccatgcc
      961 cgggcatcag cagacatatg cactgtcacc ttaaagccaa tgcgggcgct cataataccg
     1021 attgataacc ccagatttcc ggttgagccc acagcaatgc tgtattggct aaagaactgt
     1081 ttaaactccg gagaaagcag tttgctgtag tcatcttcaa gcgtcagcaa ccctgcttcc
     1141 agagccagtt tttctgcatg tgccaggact tcataaatcc cgccgcgtgc ttttatggag
     1201 ccggaaatag gcaaatggct atcttttttc agtaacagtt gcccgctgat cggttgctga
     1261 tattcctttt ccagccgttt ttgcatagcc ggaatggcaa ccagttctga ttcaataatc
     1321 cccccagtgg cagcagtttc aggaaatgct tttgccagat agggggcaaa acgagataag
     1381 cgcgcatggg cgtcctgaac atcctgttcg gtcaggccaa cataaggtaa accttcagcc
     1441 aatgaggtcg tgccaggatt gaaccaagtg gtttctttaa gagcaaccag atcctttacc
     1501 aacggatact gggcgatgag cgaattcatt ttagcgtttt ccatagtacg tctctttgcg
     1561 cttagatgat aaatgacagc aggaatgtgc ccgccagagc gatgaccgaa gcgataaatg
     1621 tcgctgtcgt atagtattta aatgtttcat tgagcgtagc gccgcaatat tgcttcacca
     1681 gccagaaaag cgagtccgta acgatcgtgc agccaatcgc accggaaccg atagcaatcg
     1741 caataatttc cgggctgatg tcgggataca gcggcagcat gggggcaaca attgccgttg
     1801 cccccatcat tgccaccgta gcggagccca ctgcagcatg cagaataagt gccaccaacc
     1861 aggccagaag aatcgggtgc atatgcatat tggagagaat aactgccagc gtatcagcga
     1921 gactgctgct ttttaaaatg gcgttgaatg cgcctccggc cccgataatc agcaaaatat
     1981 tagcaataga accgaaacca ttttccgtat gtgtgagcat cgtccccatg ctcatatgct
     2041 gacgtatacc caacacataa taggcgacaa acacggcgat aaacatggca gtgatagggt
     2101 tgccaataaa ctcaagcaag gtatacaaac cactctcacg cgccatattc aattcggcaa
     2161 tcgttttaac caacatcagc gcaatgggta gcagtatggt gaataacgtt gcgcctaatg
     2221 aaggtagtgt tttttcatcg cgaactttga gatcggcaaa ctctgtgggt acaggtttaa
     2281 agggcagtcg ttgccccaga aatttaagga aaagtgggcc accgatcagt gatgccatca
     2341 gcccaaccag caaaccgtag acgatcaccg aaccgatatc tgcgcccagc ttattggcaa
     2401 catataaagc agccggatgt gggggaacca cgcagtgcac agccatcaat gcggtacaca
     2461 gcggaatggc cagctttaac aatgaggtat tggttttttt ggcaattgaa aaagccagag
     2521 gaatcaatag cacgacgccc acttcaacaa acagcgtgat gccgcaaatc aggccaacca
     2581 gcaccataat gacatcagcc gaaagccacc ggcagcgttg aagtgtcaga ccaattcttt
     2641 ctgcggcccc ggatacttcc atcatttttc ccagtatcgt gccaaggccg ataaccgcag
     2701 cgaggaaccc cagcgttccg ccaattccac tttcaatagc atttaccata tccagtggcc
     2761 ccatacccat catcgttccc acgaagaagc tggccagcaa cagcgccaga aacgggtgga
     2821 atttgaactt cacgatggtc agtacaatta acacgatgct gataagcagc gtgctcacaa
     2881 cccagatttg agagtgcata tctcaccttc ccctgtgatt aacctgatga tattggacaa
     2941 aaaaacaacg caggctgaca aacgataaaa tttgcctttc acatgagcta aattgcatca
     3001 agggagtgat ttacatctaa aaatgaaggt gaattgagat atggttcact ttagctcacc
     3061 ttacagctga aattttgccg ctattttttt acacttaata gaaaaatgag gtgattatgg
     3121 aaccccttcg tgaaataaga aatcgactgc ttaacggctg gcaactatca aaactgcata
     3181 cttttgaagt ggctgccagg catcagtctt tcgccctggc ggcagaggaa ttgtcgctga
     3241 gccccagtgc ggtaagtcac cgtatcaatc agctggaaga agaattaggt attcagttgt
     3301 ttgttcgttc ccatcgcaaa gtggaattaa cgcacgaggg gaaacgcgtt tattgggcgc
     3361 taaaatcgtc gctggatacc ctgaatcagg aaattctgga tatcaaaaat caggagttat
     3421 cgggaacgtt aactctgtat tcccggccct ctatcgccca atgctggttg gtgcccgcat
     3481 taggtgactt tacacgccga tatccgtcta tttcgctcac cgtgctcact ggtaatgaca
     3541 atgtcaattt gcaacgtgcc ggaatcgatt tggcgatcta ctttgatgat gcgccgtcag
     3601 cgcaactgac ccataacttt ctgatggatg aagaaatctt gccagtttgt agcccggagt
     3661 acgctcatag acatgattta accaacacgg taattaacct gcgtcactgt acgttgctcc
     3721 atgacagaca ggcatggagc aacgactccg gtacggatga atggcatagt tgggcgcaac
     3781 attatgcggt taatttgccg acatcttctg gaattggctt tgatcgttct gatttagccg
     3841 ttatcgccgc gatgaatcat gttggggtgg cgatgggaag gaaacgcctg gtacaaaaaa
     3901 ggcttgccag tggtgagctc gtcgcgccgt ttggcgatat gacggtgaaa tgccatcagc
     3961 attattacat caccacatta ccgggcaggc agtggccaaa aattgaggca tttattactt
     4021 ggttaagaga acaggtaaaa accacttcat aatgaataag accacgagtt cctggtgtgc
     4081 cttattcggc actgctagtg cgaatttaat aatttacggt ttttacgtgg ttttagggat
     4141 aaacacaaga atggtgtccc ctgcaggaat cgaacctgca attagccctt aggaggggct
     4201 cgttatatcc atttaactaa gaggacaatg cggcatgagt atacccgcta atggactacg
     4261 gggtaagtac acagccgctc gattgcttaa accctcgcca tttatgccgg gtttttatca
     4321 tttttcttaa tgttttccgc acgttctgct ttttgacgtg cttctgcttt gcgcttgttg
     4381 ctcatgtcgt tacgaatctg tgcatgactc attaacgcga agataaaggt gccgccgcag
     4441 atgttccccg ctaaagtagg tagtgcgaag ggccagatga aatcgctcca gtgcagcgtg
     4501 ccgttaaaca ccagatagag gatttcaaca gaaccgacaa cgatgtgggt ggtgtcaccc
     4561 agggcaataa gccaggtcat caatataatc accacaatct ttgccgcacc cgctgcagga
     4621 aacatccata ccatagtggc gatcagccag ccggaaataa tcgcgttggc aaacatctcg
     4681 ctgggggtgt tcttcatcac atccatgccg attttgacaa atgcatcgcg agtttcttca
     4741 ttgaagatag gcatatattc aaatgcccac gcagcaatac ctgtcccgag aatattaccc
     4801 agcagcacga tgccccataa ccgcataagt aagccgacgt tgctcattgt cggtttttgc
     4861 atgacgggta gtaccgcagt tacggtgttt tcggtaaata attgctggcg ggccattatg
     4921 acgataataa aaccaaaggt ataaccgaga ttctccagca agaagctgcc cggcacgcct
     4981 tccagttcga catgaaatat cccttttgcc agtaacgaag cgcccatcga caggcccgcc
     5041 gcaatggctg accacagtag cgccattgcg tcgcgttcca gctctttttc accatcctgg
     5101 cggatatgct catgaattgc catcgcccgg gaggggagtc ggtcttcatc tatttctatt
     5161 tttttaccgc gctctttttc ttcgctctca acttcaattt cgtcgctgtg ttgatcaatt
     5221 ttgtcgttgt ccatggtctc ttctcttgaa ttagcacgta tagctaaagc gtagcggctt
     5281 ttttgctcgc aactggcggg agttactctg aaaatgtaga aaaggccgcg tttgcctttt
     5341 tctgtttcta ttgaatcaag tagcctacag ggcggcgatt accaggctat gatcaaatca
     5401 gcaaatcagg gcgtctggac atcagttgac gtgctgttac aatcgcccac acctaaacag
     5461 gcggatacgg tatcgttccg tcatggatgg caaactgcat aagccataaa aaaacaggga
     5521 gacatttatg aagcttcgcc tgtcggcgct tgctctggga actacgcttc tggtggggtg
     5581 tgcgagttcc ggtacagatc agcaagggcg ttctgacccg ttagaagggt ttaaccgcac
     5641 tatgtacaac ttcaacttca atgtattaga cccgtatatt gttcggccgg tcgctgtcgc
     5701 ctggcgtgat tatgttccgc aacctgcgcg taacggtttg agcaacttta ccggcaacct
     5761 tgaagaacct gcggtgatgg ttaactactt cttgcagggc gacccttatc aggggatggt
     5821 ccactttacc cgctttttcc tgaataccat tttgggaatg ggcggcttta ttgatgttgc
     5881 agggatggcg aacccgaaac tgcaacggac tgaacctcac cgcttcggta gtacgcttgg
     5941 tcattatggt gtgggttatg ggccgtacgt tcagttaccg ttctacggta gcttcacgct
     6001 gcgtgatgac ggtggtgata tggcggatag tctttacccg gttctttcct ggctgacctg
     6061 gccgatgtct gtgggtaaat ggacgcttga agggatagaa actcgtgcgc agttgttgga
     6121 ttccgacggt ctgctgcgtc agtcctccga tccttatatt atggtgcgtg aagcgtactt
     6181 ccagcgtcat gatttcatcg ctaatggcgg tgaactcaaa ccgcaggaaa atccgaacgc
     6241 gcaagcgatt caggatgatt taaaagatat cgattctgaa taagaaacaa acaaaaaagg
     6301 tgagtcgcaa aactcacctt ttttcttatt aagctaacgc gttatcagaa cgcgtagtta
     6361 aagttagtac cgaacagcca ggctttacct tcagactcga aggaataagc tgccgctcct
     6421 tcaccttcgg tgaatttcac tttctgacca tgcatataag atgcgccaac atcaatggat
     6481 gcatcttcat taaatgcgta ggttaaccca gcacttaacc acaaacgatc ctggtcagga
     6541 atggaaatag aacgtttatc tgccggaacc gggctgtcgt cataagcgat accggtacgg
     6601 aaggtccagt ttttatcata atagtaggtt gtacccaacg cgatacgata cgcgtcccgg
     6661 aatccttcat ctttatagaa cagagtctga ccactatcgc ctttcgcttt caattcctgg
     6721 aactggctcc aactggtata ggccatgctg tagtgaattg cccactgcgg cgcaacacgg
     6781 ttataaccag aaatctccca catctcaggc aggttcagcg ttaaggatcc attttgggtt
     6841 gcccccatcg tgccatacgg taagcccatg ttacccaaaa tattgttcag atttgggtta
     6901 atggagcttt tgtaatcccc atcgaagtcg attttaactt ctgaacgata tgtcagcccc
     6961 caacggttat ctttatccag ttcataaaga atacccgcgt tccagccaaa gccccattcg
     7021 tcacctttca aatgagcaat ttgagagtcc ggagtattaa taccattggt taatgaagcg
     7081 ggtacaccct gggcaatcaa ctgttgagac aacaatggcc ccagcgaacc ggcgtaacgc
     7141 tcaatttttg ccttggcata aactgcatca aaaccaagac caaaactcca gctattgtta
     7201 aggcgataag ccccgctaag gttaaggttg attgtttgca gatcggtttt accgccatag
     7261 ataccagcag cgtagttgtc ggtaaattcc gtagccaggc catagttgga agttattgac
     7321 gctccccaac caaactgatc gttaataggc gcgacaaagt gcaggttagg tacccacgct
     7381 gtcggcgcga tatttttagc atttgcactc tgaccggtta aattagaatg tccggtaata
     7441 tctacatccg gatcaatata aaccgcaccc gcagaaaatg tcgggcggtc aaacatggta
     7501 atcaatgcgg ggttacggct aacgttacct gcatcatcgg caattgcacc ttcccctgaa
     7561 taagcccggc ccaggccaga ggaagaaaat tcgtttaact gaaagcctgc cgaccaggcc
     7621 tgggtggaga taagtgccac tgcgactgcg agagcagact ttgtaaacag ggttttctgg
     7681 ctcatgacca taacctcaat gatttatttt tatacaaact atgttacgtg ctgtaacagg
     7741 agcgcgaagt gtagggtctg aggtacatct tagaaatcag accagtggcg agagtatagg
     7801 tcggaccagc tgggatgttg caagtatgtt tctatctttt ttcagatatg atctaagaaa
     7861 cgcgatccag ctggcaaaat tgggaggtgg cgcactcatg gattcgggtg aattttgttt
     7921 tagatcattt ttaagtgtga tttcggtcac ttatccgatt ttgcagaatt aacaactgga
     7981 gcagcacttt ccggaagcgt aaaatatcca caacgtttat ctggcacctt gggtgcacat
     8041 acagaggaaa tcaacgatga gtaaatgtag tgctgatgaa accccggttt gctgctgtat
     8101 ggatgttggc accattatgg acaactccga ctgcaccgcg tcttacagcc gtgtgtttgc
     8161 aaaccgcgca gaagcagaac aaacgctggc ggcgctgact gaaaaagccc gtagcgtgga
     8221 atccgaaccg tgcaaaatca ccccgacttt cactgaagag tctgacggtg tgcgcctgga
     8281 tatcgatttc acttttgcct gcgaagctga aatgctgatc ttccagctgg gtctgcgtta
     8341 attcctctcg ggtacatacg cccctgtcag ctgctgacag gggcgtttta ttttctcctc
     8401 ctcttttctc tctgtgtcat agctcccact ttcccttttc tccacttggc taaatgtaaa
     8461 aaaatggtta agactatgat caggtcagac cactttattt atttttttac aggggagtgt
     8521 tatgggtcag gttttaccgc tggttacccg ccagggcgat cgtatcgcca ttgttagcgg
     8581 tttacgtacg ccttttgccc gacaggcgac ggcttttcat ggcattcccg cggttgattt
     8641 agggaagatg gtggtaggcg aactgctggc acgcaccgag atccccgctg aagtgattga
     8701 acaattggtc ttcggtcagg ttgtgcaaat gccggaagcg cccaacattg cccgtgaaat
     8761 tgttcttggc actggaatga atgtgcatac tgatgcttac agcgtcagcc gcgcttgcgc
     8821 caccagtttt caggcggttg cgaacgttgc cgaaagcctg atggcgggaa ctattcgagc
     8881 ggggattgcc ggtggggcag attcctcttc cgtattgcca attggcgtca gtaaaaaact
     8941 ggcgcgcgta ctggttgatg tcaacaaagc ccgcacaatg agccagcgcc tgaaactctt
     9001 ctctcgcctg cgtttgcgcg acttaatgcc cgtaccgcct gcggtagcag aatattctac
     9061 cggcttgcgg atgggggaca ccgcagagca aatggcgaaa acctacggca tcacccgaga
     9121 acagcaagat gcactggcgc accgttcgca tcagcgtgcc gctcaggcgt ggtcagaagg
     9181 taaactcaaa gaagaggtga tgactgcctt tatccctcct tataaacaac cgcttgtcga
     9241 agacaacaat attcgcggta attcctctct tgctgattac gcaaagttgc gtccggcgtt
     9301 tgatcgtaaa cacgggacgg taacggcggc gaacagtacg ccgctgaccg atggcgcggc
     9361 agcggtgatc ctgatgactg aatcccgggc gaaagaatta gggctggtac cgctgggcta
     9421 tctgcgcagc tacgcattta ctgcgataga tgtctggcag gacatgttgc tcggtccagc
     9481 ctggtcaaca ccgctggcgc tggaacgtgc cggattgacg atgggcgatc tgacattgat
     9541 cgatatgcac gaagcctttg ccgcccagac gctggccaat attcagttgc tgggtagcga
     9601 acgttttgct cgcgacgtac tggggcgtgc acatgccact ggcgaagtgg acgagagcaa
     9661 atttaacgtg cttggcggtt cgattgctta cgggcatccc ttcgcagcga ccggcgcgcg
     9721 gatgattacc cagacattgc atgaacttcg ccgtcgtggc ggtggatttg gtttagtgac
     9781 cgcctgtgct gcgggtgggc ttggcgcagc aatggttctg gaggcggaat aatggaaatg
     9841 gcatcagcgt ttactcttaa tgttcgtctg gacaacattg ctatcatcac catcgacgta
     9901 ccgggtgaga aaatgaatac cctgaaggcg gaatttgcct cgcaggtacg cgccattatt
     9961 aagcaactcc gtgaaaacaa agagttgcgt ggcgtggtgt ttgtctccgc gaaaccagac
    10021 aacttcattg ctggcgcaga catcaacatg atcggcaact gcaaaacggc gcaagaagcg
    10081 gaagtactgg cgcggcaggg gcaacagtta atggcggaga ttcatgcttt gcccattccg
    10141 gttatcgcgg ctattcatgg cgcttgcctg ggcggcgggc tggagttggc gctggcgtgc
    10201 cacggtcgtg tttgtactga cgatcctaaa acggtgctcg gtttgcctga agtacaactt
    10261 ggattgttac ccggttcagg aggtacccag cgtttaccgc gcctgatagg tgtcagcaca
    10321 gcattagaga tgatcctcac cggaaaacaa cttcgggcga aacaggcggt aaagctgggg
    10381 ttggtggatg acgtcgttcc gcactccata ttgctggaag ccgctgttga gctggcaaag
    10441 caggatcgcc catcttcacg ccctctacct gttcgcgagc gtattctggc ggggccgtta
    10501 ggtcgtgcgc tgctgttcaa aatggtcggc aagaaaacag aacacaaaac gcaaggtaac
    10561 tatccggcga cagaacgcat cctggaggtt gtcgaaacgg gattagcgca ggggaccagc
    10621 agtggctatg acgccgaagc tcgggcgttt ggtgaactgg cgatgacgcc gcaatcgcag
    10681 gcgctgcgca atatcttttt tgccagtacg gacgtgaaaa aagatcccgg cagtgatgcg
    10741 ccgcctgcgc cattaaacag cgtggggatt ctaggtggtg gcttgatggg cggcggtatt
    10801 gcttatgtca ctgcttgtaa agcggggctt ccggtcagaa ttaaagatat caacccgcag
    10861 ggcataaatc atgcgctgaa gttcagttgg gatcagcttg agggcaaagt tcgccgtcgt
    10921 catctcaaag ccagcgaacg tgacaaacag ctggcattaa tctccggaac gacggactat
    10981 tgcggctttg cccatcgcga tctgattatt gaagcggtgt ttgaaaatct cgaattaaaa
    11041 caacagatgg tggcggaagt ggagcaaaat tgcgctactc ataccatctt tgcttcgaac
    11101 acgtcatctt taccgattgg cgatatcgcc gctcacgcca cgcgacctga gcaagttatt
    11161 ggcctgcatt tcttcagtcc ggtggaaaaa atgccgctgg tggagattat tcctcatgcg
    11221 agtacatcgg cgcaaaccat cgccaccacg gtaaaactgg cgaaaaaaca gggcaaaatg
    11281 ccaattgtcg tgcgtgacaa agctgggttt tacgtcaatc gcatcttagc gccttacatc
    11341 aatgaagcta tccgcatgtt gaccgaaggc gagcgtattg agcatatcga tgccgcgcta
    11401 gtgaaatttg gttttccggt aggcccaatc caacttttgg atgaggtagg aatcgacaca
    11461 gggactaaaa ttatgcctgt actggaagcc gcttatggag aacgttttag cgcgtctgca
    11521 aatgttgttt cttcaatttt gaacgacgat cgcaaaggca gaaaaaatgg tcggggtttc
    11581 tatctttatg gtcagaaagg gcgtaaaagc aaaaaacagg tcgatcccgc catttacccg
    11641 ctcattggcg tacaagggca ggggcgactc tccgcaccgc aggttgctga acggtgtgtg
    11701 atgttgatgc tgaatgaagc agtacgttgt ctggacgagc aggttatccg tagcgtgcgt
    11761 gacggggata ttggtgcggt atttggcatt ggttttccgc catttctcgg tggaccgttc
    11821 cgctatatcg attctctcgg cgcaggcgaa gtggttgcaa taatgcaacg actggccacg
    11881 cagtatggtt cccgttttac cccttgcgat cgtttagttg agatgagcga gcgtggggaa
    11941 agtttttgga aaacaactgc aactgacctg caataagaag gtcaaagtta tatgaatccg
    12001 cgctgaatgg cggagtgttg ctcaaaatgt aaacgcatat tgactatact tacgccattg
    12061 aggtaaaaaa cagcgtttca ttcggtgaat ggataaggca caatgccggc caccgttttc
    12121 tttctctggt ttcagatgaa agaaaacggg cgaatctggt taacaaaagc ggtgcaatat
    12181 gcaagttttt atcatgcgtc acggcgacgc agccctcgat gccgccagtg attcggttcg
    12241 tcctctgacc actcatggtt gtgacgaatc tcgcctgatg gcgaactggc tgaaaggtca
    12301 aaaagtggaa atcgaacgcg ttctggtgag cccgttcctg cgagccgagc aaacacttga
    12361 agaagtaggg gattgtctga acctgccttc cagtgcagaa gttctgccgg agttaacgcc
    12421 ctgcggcgat gttggtctgg tcggtgccta tttgcaggcg ttgaccaatg aaggtgttgc
    12481 ttcggtactg gtgatctccc acttaccgtt agttgggtat ctggttgccg agttatgtcc
    12541 gggcgaaacg ccgcctatgt ttaccacgtc agcgattgcc agcgtaacgc ttgatgaaag
    12601 cggaaaaggc caattcaact ggcagatgag cccttgtaat ttgaagatgg caaaagcaat
    12661 ttaagcaatc aatgcccggt tacgtgcgcg tacaccgggc agtttctccg tcatacaatc
    12721 attctttttt tgtctaaatg ttgttaatat ttaacaactc tcgatagtta ttttcccatt
    12781 attgaatccc gtaataaaat aaagaagata a
//
LOCUS       DAHHTG010000120        12542 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.121, whole genome shotgun sequence.
ACCESSION   DAHHTG010000120 DAHHTG010000000
VERSION     DAHHTG010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12542)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12542)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12542
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.121"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            <1..96
                     /locus_tag="NBI74_003568"
     CDS             <1..96
                     /locus_tag="NBI74_003568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HCD1933848.1"
                     /translation="ARLGRKLLSFSKSVELHDKVIGHYLNIKHYQ"
     gene            complement(271..1044)
                     /gene="uxuR"
                     /locus_tag="NBI74_003569"
     CDS             complement(271..1044)
                     /gene="uxuR"
                     /locus_tag="NBI74_003569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Uxu operon transcriptional regulator"
                     /protein_id="HCD1933849.1"
                     /translation="MKSATSAQRPYQEVGAMIRDLIIKTPYNPGERLPPEREIAEMLD
                     VTRTVVREALIMLEIKGLVEVRRGAGIYVLDSSGSQNTDGPDANVCNDAGPFELLQAR
                     QLLESNIAEFAALQATREDIVKMRQALQLEERELASSAPGSSESGDMQFHLAIAEATH
                     NSMLVELFRQSWQWRENNPMWIQLHSHLDDSLYRKEWLGDHKQILAALIKKDARAAKL
                     AMWQHLENVKQRLLEFSNVDDIYFDGYLFDSWPLDKVDA"
     gene            complement(1259..2719)
                     /locus_tag="NBI74_003570"
     CDS             complement(1259..2719)
                     /locus_tag="NBI74_003570"
                     /EC_number="1.1.1.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructuronate reductase"
                     /protein_id="HCD1933850.1"
                     /translation="MTTIVDSNLPVARPSWDHSRLESRIVHLGCGAFHRAHQALYTHH
                     LLESTDSDWGICEVNLMPGNDRVLIENLKKQQLLYTVAEKGAESTELKIIGSMKEALH
                     PEIDGCEGILNAMARPQTAIVSLTVTEKGYCADAASGQLDLNNPLIKHDLENPTAPKS
                     AIGYIVEALRLRREKGLKAFTVMSCDNVRENGHVAKVAVLGLAQARDPQLAAWIEENV
                     TFPCTMVDRIVPAATPETLQEIADQLGVYDPCAIACEPFRQWVIEDNFVNGRPDWDKV
                     GAQFVADVVPFEMMKLRMLNGSHSFLAYLGYLGGYETIADTMTNPAYRKAAFALMMQE
                     QAPTLSMPEGTDLNAYATLLIERFSNPSLRHRTWQIAMDGSQKLPQRLLDPVRLHLQN
                     GGSWRHLALGVAGWMRYTQGVDEQGNAIDVVDPMLAEFQKINAQYQGADRVKALLGLS
                     GIFADDLPQNADFVGAVTAAYQQLCERGARECVAAL"
     gene            complement(2800..3984)
                     /gene="uxuA"
                     /locus_tag="NBI74_003571"
     CDS             complement(2800..3984)
                     /gene="uxuA"
                     /locus_tag="NBI74_003571"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HCD1933851.1"
                     /translation="MEQTWRWYGPNDPVSLADVRQAGATGVVTALHHIPNGEVWSVEE
                     ILKRKAIVEDAGLVWSVVESVPIHEDIKTHTGNYEQWIANYQQTLRNLAQCGIRTVCY
                     NFMPVLDWTRTDLEYVLPDGSKALRFDQIEFAAFEMHILKRPGAEADYTEEEIAQAAV
                     RFATMSDEDKARLTRNIIAGLPGAEEGYTLDKFRKHLELYKDIDKAKLRENFAVFLKA
                     IIPVAEEVGVRMAVHPDDPPRPILGLPRIVSTIEDMQWMVDTVNSMANGFTMCTGSYG
                     VRADNDLVDMIKQFGPRIYFTHLRSTMREDNPKTFHEAAHLNGDVDMYEVVKAIVEEE
                     HRRKAEGKEDLIPMRPDHGHQMLDDLKKKTNPGYSAIGRLKGLAEVRGVELAIQRAFF
                     SR"
     gene            4324..5667
                     /gene="gntP"
                     /locus_tag="NBI74_003572"
     CDS             4324..5667
                     /gene="gntP"
                     /locus_tag="NBI74_003572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate permease GntP"
                     /protein_id="HCD1933852.1"
                     /translation="MHVLNILWVVFGIGLMLVLNLKFKINSMVALLVAALSVGMLAGM
                     DLMSLLHTMKAGFGNTLGELAIIVVFGAVIGKLMVDSGAAHQIAHTLLARLGLRYVQL
                     SVIIIGLIFGLAMFYEVAFIMLAPLVIVIAAEAKIPFLKLAIPAVAAATTAHSLFPPQ
                     PGPVALVNAYGADMGMVYIYGVLVTIPSVICAGLILPKFLGNLERPTPSFLKADQPVD
                     MNNLPSFGVSILVPLIPAIIMISTTIANIWLVKDTPAWEVVNFIGSSPIAMFIAMVVA
                     FVLFGTARGHDMQWVMNAFESAVKSIAMVILIIGAGGVLKQTIIDTGIGDTIGMLMSH
                     GNISPYIMAWLITVLIRLATGQGVVSAMTAAGIISAAILDPATGQLVGVNPALLVLAT
                     AAGSNTLTHINDASFWLFKGYFDLSVKDTLKTWGLLELVNSVVGLIIVLIISMVA"
     gene            complement(5848..6750)
                     /gene="fimH"
                     /locus_tag="NBI74_003573"
     CDS             complement(5848..6750)
                     /gene="fimH"
                     /locus_tag="NBI74_003573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria D-mannose specific adhesin FimH"
                     /protein_id="HCD1933853.1"
                     /translation="MKRVITLFAVLLMGWSVNAWSFACKTANGTAIPIGGGSANVYVN
                     LAPAVNVGQNLVVDLSTQIFCHNDYPETITDYVTLQRGSAYGGVLSNFSGTVKYSGSS
                     YPFPTTSETPRVVYNSRTDKPWPVALYLTPVSSAGGVAIKAGSLIAVLILRQTNNYNS
                     DDFQFVWNIYANNDVVVPTGGCDVSARDVTVTLPDYPGSVPIPLTVYCAKSQNLGYYL
                     SGTTADAGNSIFTNTASFSPAQGVGVQLTRNGTIIPANNTVSLGAVGTSAVSLGLTAN
                     YARTGGQVTAGNVKSIIGVTFVYQ"
     gene            complement(6770..7273)
                     /gene="fimG"
                     /locus_tag="NBI74_003574"
     CDS             complement(6770..7273)
                     /gene="fimG"
                     /locus_tag="NBI74_003574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimG"
                     /protein_id="HCD1933854.1"
                     /translation="MKWRKRGYLLAAILALASATIQAADVTITVNGKVVAKPCTVSTT
                     NATVDLGDLYSFSLMSAGAASAWHDVALELTNCPVGTSRVTASFSGAADSTGYYKNQG
                     TAQNIQLELQDDSGNTLNTGATKTVQVDDSSQSAHFPLQVRALTVNGGATQGTIQAVI
                     SITYTYS"
     gene            complement(7286..7816)
                     /gene="fimF"
                     /locus_tag="NBI74_003575"
     CDS             complement(7286..7816)
                     /gene="fimF"
                     /locus_tag="NBI74_003575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimF"
                     /protein_id="HCD1933855.1"
                     /translation="MRNKPFYLLCAFLWLAVSHALAADSTITIRGYVRDNGCSVAAES
                     TNFTVDLMENAAKQFNNIGATTPVVPFRILLSPCGNAVSAVKVGFTGVADSHNANLLA
                     LENTVSAAAGLGIQLLNEQQNQIPLNAPSSAISWTTLTPGKPNTLNFYARLMATQVPV
                     TAGHINATATFTLEYQ"
     gene            complement(7826..10462)
                     /gene="fimD"
                     /locus_tag="NBI74_003576"
     CDS             complement(7826..10462)
                     /gene="fimD"
                     /locus_tag="NBI74_003576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher FimD"
                     /protein_id="HCD1933856.1"
                     /translation="MSYLNLRLYQRNTQCLHIRKHRLAGFFVRLFVACAFAAQAPLSS
                     AELYFNPRFLADDPQAVADLSRFENGQELPPGTYRVDIYLNNGYMATRDVTFNTGDSE
                     QGIVPCLTRAQLASMGLNTASISGMNLLADDACVPLTAMIHDATAHLDVGQQRLNLTI
                     PQAFMSNRARGYIPPELWDPGINAGLLNYNFSGNSVQNRIGGNSHYAYLNLQSGLNIG
                     AWRLRDNTTWSYNSSDRSSGSKNKWQHINTWLERDIIPLRSRLTLGDGYTQGDIFDGI
                     NFRGAQLASDDNMLPDSQRGFAPVIHGIARGTAQVTIKQNGYDIYNSTVPPGPFTIND
                     IYAAGNSGDLQVTIKEADGSTQIFTVPYSSVPLLQREGHTRYSITAGEYRSGNAQQEK
                     PRFFQSTLLHGLPAGWTIYGGTQLADRYRAFNFGIGKNMGALGALSVDMTQANSTLPD
                     DSQHDGQSVRFLYNKSLNESGTNIQLVGYRYSTSGYFNFADTTYSRMNGYNIETQDGV
                     IQVKPKFTDYYNLAYNKRGKLQLTVTQQLGRTSTLYLSGSHQTYWGTSNVDEQFQAGL
                     NTAFEDINWTLSYSLTKNAWQKGRDQMLALNVNIPFSHWLRSDSKSQWRHASASYSMS
                     HDLNGRMTNLAGIYGTLLEDNDLSYSVQTGYAGGGDGNSGSTGYATLNYRGGYGNANI
                     GYSHSDDIKQLYYGVSGGVLAHANGVTLGQPLNDTVVLVKAPGAKDAKVENQTGVRTD
                     WRGYAVLPYATEYRENRVALDTNTLADNVDLDNAVANVVPTRGAIVRAEFKARVGIKL
                     LMTLTHNNKPLPFGAMVTSESSQSSGIVADNGQVYLSGMPLAGKVQVKWGEEENAHCV
                     ANYQLPPESQQQLLTQLSAECR"
     gene            complement(10529..11254)
                     /gene="fimC"
                     /locus_tag="NBI74_003577"
     CDS             complement(10529..11254)
                     /gene="fimC"
                     /locus_tag="NBI74_003577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418736.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria chaperone FimC"
                     /protein_id="HCD1933857.1"
                     /translation="MSNKNVNVRKSQEITFCLLAGILMFMAMMVAGRAEAGVALGATR
                     VIYPAGQKQVQLAVTNNDENSTYLIQSWVENADGVKDGRFIVTPPLFAMKGKKENTLR
                     ILDATNNQLPQDRESLFWMNVKAIPSMDKSKLTENTLQLAIISRIKLYYRPAKLALPP
                     DQAAEKLRFRRSANSLTLINPTPYYLTVTELNAGTRVLENALVPPMGESTVKLPSDAG
                     SNITYRTINDYGALTPKMTGVME"
     gene            complement(11291..11830)
                     /locus_tag="NBI74_003578"
     CDS             complement(11291..11830)
                     /locus_tag="NBI74_003578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418735.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCD1933858.1"
                     /translation="MKRKRLFLLASLLPMFALAGNKWNTTLPGGNMQFQGVIIAETCR
                     IEAGDKQMTVNMGQISSNRFHAVGEDSAPVPFVIHLRECSTVVSERVGVAFHGVADGK
                     NPDVLSVGEGPGIATNIGVALFDDEGNLVPINRPPANWKRLYSGSTSLHFIAKYRATG
                     RRVTGGIANAQAWFSLTYQ"
     gene            complement(11895..12443)
                     /gene="fimA"
                     /locus_tag="NBI74_003579"
     CDS             complement(11895..12443)
                     /gene="fimA"
                     /locus_tag="NBI74_003579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HCD1933859.1"
                     /translation="MKIKTLAIVVLSALSLSSAAALADTTTVNGGTVHFKGEVVNAAC
                     AVDAGSVDQTVQLGQVRTASLKQAGATSSAVGFNIQLNDCDTTVATKAAVAFLGTAID
                     ATHTDVLALQSSAAGSATNVGVQILDRTGAALALDGATFSSETTLNNGTNTIPFQARY
                     FATGAATPGAANADATFKVQYQ"
BASE COUNT         2962 a         3297 c         3081 g         3202 t
ORIGIN      
        1 gcacggctgg gacggaagtt gctgtcgttc tcaaaatcgg tggagctgca tgacaaagtc
       61 atcgggcatt atctgaacat aaaacactat caataagttg gagtcattac cgaattatat
      121 tggtaaatag ccactgagaa gaatgagtac tcacgttcag gttatacaga tagctaaaca
      181 ataccctgca atttacaaag taatactcat tgcgacttct taatttaaga aagcgcaagg
      241 aacgtttacc cttgcgctta ttataataag tcaggcgtcg actttatcca gcggccatga
      301 atcaaacaga tagccgtcaa aataaatatc gtcaacgttc gagaattcca gcagacgttg
      361 cttaacgttt tccagatgct gccacattgc cagcttcgcc gctcgggcat cttttttgat
      421 taacgcggcg aggatctgtt tgtgatcgcc caaccactct ttgcgataca ggctgtcatc
      481 cagatggctg tgcaactgga tccacatcgg attgttttcc cgccactgcc aggactgacg
      541 gaacagctcc accagcatgc tgttatgcgt cgcttcggca atggcgagat ggaattgcat
      601 gtcaccactt tcgctgctgc ccggcgcgct ggaagccagt tcacgctctt ccagttgcaa
      661 tgcctgacgc attttgacga tatcttcgcg ggtagcctgc agggcggcaa attccgcgat
      721 gttactttcc agtaactgac gcgcctgtaa cagctcaaaa ggaccggcat cattgcagac
      781 gttggcatcc ggaccgtctg tgttctggct gcctgagcta tcaagaacat agataccggc
      841 accccgacgc acttccacca gccctttgat ctccagcatg atcagcgctt cacgtaccac
      901 cgtccgcgtg acatcaagca tttctgcaat ttcacgctcc ggcggcagcc gttcgccagg
      961 gttgtacggc gtctttatga tcagatcgcg gatcatcgcc ccgacttcct ggtaaggtct
     1021 ttgcgcagag gtggcagatt tcatggtgtt gtgtccgtta ggtgcggtta atcaaatttt
     1081 tgccggatgc ggcgtgaacg ccttatccgg cctacaacgg gcaattttac ggcccccgtt
     1141 aatctcaaac tataccatta gcactggaag ttggcacgta caggtgggca aaaaaacgaa
     1201 aactggtcaa ccaaatatca gtttgattga ccagtaagtc tgtagggtaa tcagttagtt
     1261 acagcgcagc cacacactcg cgcgcaccgc gttcgcacag ctgctgatat gccgccgtca
     1321 ccgcgccaac aaagtcggca ttctgcggca gatcatcggc aaaaataccg ctcaggccca
     1381 gcagcgcttt cacgcggtct gcgccctgat actgcgcgtt gatcttctgg aactccgcca
     1441 gcatcgggtc gaccacgtca atggcattac cctgctcatc cacgccctgg gtgtaacgca
     1501 tccagccagc cacgcccagc gccaggtgac gccagctgcc gccgttttgc aggtgcagac
     1561 gcaccgggtc cagcagacgc tgcggtaact tctggctgcc gtccatcgca atctgccagg
     1621 tacggtgacg cagagacggg ttgctgaaac gctcgatcag cagcgtcgca taggcgttca
     1681 ggtctgtacc ttccggcatc gacagcgttg gcgcttgttc ctgcatcatc agggcaaagg
     1741 ccgctttgcg ataagccggg ttagtcatgg tgtcggcaat ggtttcatag ccgccgaggt
     1801 aaccgaggta cgccagaaaa gagtggctgc cgttcagcat acgcagcttc atcatttcga
     1861 acggcacaac gtctgcaacg aactgtgcgc ccactttatc ccagtccggg cgaccattaa
     1921 cgaagttatc ttcaatcacc cactgacgga acggttcgca ggcaatggcg cacgggtcgt
     1981 aaacacccag ctggtcagca atttcctgta aggtttctgg cgtcgccgcc ggaacgatgc
     2041 ggtcaaccat ggtgcacggg aaggtgacat tttcttcaat ccatgccgcc agctgcgggt
     2101 cacgcgcctg agccagcccc agtaccgcga ccttcgccac atgaccgttt tcacgcacgt
     2161 tatcgcagga catcaccgta aacgctttca accctttttc acgacgcaga cgcaaggctt
     2221 cgacgatgta accaatcgcg gacttcggcg cagtcgggtt ttccagatcg tgcttgatca
     2281 gtgggttatt gagatccagt tgaccgcttg ccgcatcagc gcagtagcct ttttccgtga
     2341 ccgttagaga gacaatcgcc gtttgcggac gcgccatcgc gttgagaata ccttcgcagc
     2401 catcgatttc cggatgcagc gcttctttca tcgaaccgat aattttcagc tcggtgctct
     2461 ctgcgccttt ttccgctacg gtgtacagca gttgctgttt tttcaggttt tcgatcagca
     2521 cgcggtcgtt gcctggcatc aggttaactt cgcagatgcc ccagtcgctg tcggtgcttt
     2581 ccagcagatg atgggtatac agcgcctggt gcgcgcggtg aaacgccccg caaccgagat
     2641 gcacaatgcg tgattccaga cgagaatgat cccatgacgg gcgggcaacc ggcagattgc
     2701 tgtcaacaat agtagtcatc gtaaactcca gaatccgtct tcaaagagtg acggcaagga
     2761 agaaggaacc ggcacggcgc gcagccatgc cggtggatat taacggctaa agaaagcgcg
     2821 ctggatcgcc agttcgacac cgcgaacttc ggccaggcct ttcagacgac caattgcgga
     2881 gtaacctggg ttggttttct tcttcaggtc gtccagcatc tgatgaccgt ggtccggacg
     2941 catcgggatc aggtcttctt tgccttccgc tttacgacgg tgttcttctt caacaatcgc
     3001 tttcaccact tcgtacatat caacgtcacc gttcaggtgc gccgcttcgt ggaaggtttt
     3061 cgggttatct tcacgcatgg tggagcgcag atgggtgaag taaatacgcg gaccgaactg
     3121 cttgatcata tcaaccagat cgttgtcagc acgcacgccg taggaaccgg tgcacatggt
     3181 aaaaccgttt gccatgctgt ttacggtatc aaccatccac tgcatatctt caatggtgga
     3241 aacaatgcgc ggcaggccga ggatcgggcg cggcggatcg tccgggtgaa cagccatacg
     3301 cacgccgact tcttcagcaa ctggaataat cgctttcagg aagacggcaa agttttcgcg
     3361 cagcttcgct ttgtcgatat ctttgtacag ctccaggtgt ttacggaact tgtcgagggt
     3421 gtagccttct tccgcgcccg gaagaccagc aatgatgtta cgggtcagac gcgctttgtc
     3481 ttcgtcgctc atagtggcga agcgaacagc agcctgagca atttcttctt cggtgtaatc
     3541 cgcttccgcg cctggacgtt tcaggatatg catttcgaat gcagcgaatt cgatctggtc
     3601 gaagcgcagc gctttggagc cgtctggcag cacgtattcg aggtcagtac gggtccagtc
     3661 gagcaccggc atgaagttgt agcacacggt gcgaatgccg cactgcgcca ggttgcgcag
     3721 agtctgctga tagttagcga tccactgctc atagttgcca gtgtgggttt tgatatcttc
     3781 gtgaattggt acgctttcta cgacagacca caccaggcct gcgtcttcaa cgatcgcctt
     3841 gcgtttgagg atctcttcta cggaccatac ttcgccgttc gggatatggt gcagcgcggt
     3901 aaccacgcca gttgcgcccg cctgacggac atcagctaaa gaaaccggat cgtttgggcc
     3961 gtaccagcgc caggtctgtt ccatgatttt tcctctttca catagtgggt tccacagcaa
     4021 agagacgcgg aaccctggag ttgtttaggt atccggatga acaaccagac cgcagccgtg
     4081 gcgttgcatc ggatgagatg gcgtataagt tctacctttt ggtaagacat gtcaacccga
     4141 attcttaaat tggttaacca catcacaaga atttcactct catggttgtt gctgcaaatt
     4201 gcagcaaagg gtaatcgatg gttattgatc agaggatgtg acattcatcg caacaatggt
     4261 tgaccaattt acataacatg tcgcccaaaa taacaacggt tcaaccaggt aaggtaaaac
     4321 gttatgcatg tgcttaacat tctctgggtg gtattcggca ttggcctgat gctggtactg
     4381 aatttgaagt tcaaaatcaa ttcaatggtg gctttgttgg tggcggcgct gtccgtcggg
     4441 atgctggcgg gcatggattt gatgtcgctg ctgcacacta tgaaagcggg cttcggcaac
     4501 acgctggggg aactggctat catcgtggtg ttcggtgcgg tcatcggtaa attgatggtc
     4561 gactccggcg cggctcacca gatagcgcat acgctgctgg cgcgtctcgg tctgcgctat
     4621 gtacagctgt cggtgattat catcggcctg atttttggtc tggcgatgtt ctatgaagtg
     4681 gcctttatca tgttagcgcc gctggttatt gttattgccg ccgaagctaa aattccgttc
     4741 ctgaaactgg cgatcccggc agtagcagct gccactaccg cacattcact gttcccaccg
     4801 cagccgggtc cggtggcgct ggtgaatgct tatggcgcgg atatggggat ggtttatatc
     4861 tatggcgtac tggtgacgat cccaagtgta atctgcgcag gtctgatcct gccgaagttc
     4921 ctcggcaatc ttgagcgccc aacgccatca ttcctgaaag cagatcaacc ggtagatatg
     4981 aataatctgc cctctttcgg cgtttcgatt ctggtgccgc tgatcccggc gatcattatg
     5041 atctctacca ccatcgccaa tatctggctg gtaaaagata cccctgcctg ggaagtggtt
     5101 aactttatcg gttcctcgcc gattgcaatg tttattgcga tggtggttgc attcgtactc
     5161 tttggcaccg cacgtggtca tgacatgcag tgggtgatga acgcttttga aagcgcggtg
     5221 aagagtattg caatggtgat tctgatcatc ggtgcgggtg gcgtgctgaa gcagaccatc
     5281 atcgacaccg gcattggcga caccatcggc atgttgatgt cccacggcaa tatctcgccc
     5341 tacatcatgg catggctgat cactgtgcta attcgtctgg cgacgggtca gggtgtcgtt
     5401 tcggcgatga ccgccgccgg gattatcagt gctgcaatcc ttgatccagc aactggtcag
     5461 ctggttggcg tgaatccggc gctgctggta ctggcgacgg ctgcgggttc caacaccctc
     5521 acccacatta acgatgcttc tttctggttg ttcaaaggat actttgacct gtcagtaaaa
     5581 gacacgttga aaacctgggg tctgctggag ctggtcaact ccgtggttgg gcttattatt
     5641 gtgctgatta ttagcatggt agcgtaaaag aacaccagag cctgccaatg gcaggctctg
     5701 ttcggattgt cggcaaagta cgctttgttt atgccagatg cgacgctgac gcgtcttatc
     5761 tggcctacaa agggctaacg tgcaggtttt gtaggtctga tagcgtagcg cctcaggtac
     5821 cagcattagc aatgtgctgt aattttttta ttgataaaca aaagtcacgc caataatcga
     5881 tttcacattc cctgcagtca cctgccctcc ggtacgtgcg taatttgccg ttaatcccag
     5941 acttaccgcc gaagttccta ctgctcctaa cgataccgtg ttattcgctg gaataatcgt
     6001 accgttgcgc gtcaactgta cgccgacgcc ctgcgctggt gaaaacgacg cggtattggt
     6061 gaaaatcgag ttgcccgcat ctgcggttgt gccggagagg taatacccca ggttttggct
     6121 tttcgcacaa taaacggtaa gaggaattgg cactgaacca gggtagtccg gcagagtaac
     6181 ggtgacatca cgagcagaaa catcacagcc gccagtgggc accaccacat cattattggc
     6241 gtaaatattc cacacaaact ggaaatcatc gctgttatag ttgttggtct gtcgcaaaat
     6301 aagcacggca attaatgagc cagctttaat cgccaccccg cccgcactgc tcacaggcgt
     6361 caaataaagc gccaccggcc acggcttatc cgttctcgaa ttataaacaa cgcgcggcgt
     6421 ttcgctggtg gtaggaaatg gatagctact gccactatat tttacggtcc cggaaaaatt
     6481 agataacacg ccgccataag ccgagcctcg ttgcagtgtg acatagtctg taatggtttc
     6541 cggataatcg ttatggcaaa agatttgcgt cgaaagatct acgaccaggt tttgccccac
     6601 attcacggcg ggcgcaaggt ttacataaac attagcgctg ccaccgccaa tagggatagc
     6661 tgtaccattg gcggttttac aggcgaatga ccaggcattt accgaccagc ccatcagcag
     6721 tacagcaaac agggtaataa ctcgtttcat tacaatcatc tcttcgggtt cagctgtagg
     6781 tataggtgat gctaatcact gcctgaatgg ttccctgagt ggctccgcca tttaccgtca
     6841 atgctctgac ctgtaacggg aagtgcgctg attgtgagga atcatccacc tgaactgttt
     6901 tggttgcgcc agtattcaat gtgttgccac tgtcatcctg tagctctaac tggatgtttt
     6961 gcgcggtccc ctgattttta taatatccgg tactgtcggc tgccccgctg aagctggcag
     7021 tgaccctcga cgttcccacc ggacaattag tcaactcaag cgcaacatca tgccaggccg
     7081 atgccgcccc ggcagacata agactgaaag aataaagatc gccgagatca accgtggcat
     7141 tggtggtgga aaccgtgcac ggtttagcga cgaccttacc gttcaccgtg atggtgacat
     7201 cggctgcctg tatcgtcgca cttgcgagcg ccaatattgc cgccaataaa tacccacgtt
     7261 tgcgccattt catgagcatc tccagttact gatattcaag agtgaaggta gccgtggcat
     7321 tgatatgccc cgcagtgaca ggcacctgtg tcgccattag ccgggcgtaa aaattcagcg
     7381 tatttggttt acccggcgtc agggtcgtcc acgaaatcgc ggacgatggg gcattaaggg
     7441 gtatctgatt ttgttgctca ttcagaagct gtattcccag tcccgcagcc gctgacaccg
     7501 tattttcaag tgcaagcagg ttggcattgt ggctatctgc aacgccagta aacccaacct
     7561 ttacggcaga aacggcgtta ccacagggtg acagcaaaat acgaaatgga acgacaggag
     7621 tcgtcgcgcc aatgttgtta aattgcttcg ccgcgttttc catcagatca acagtaaaat
     7681 tggttgattc agcggccaca ctacagccat tatctctgac atagccgcgg atagtaatcg
     7741 tgctatccgc agccaaagcg tgacttaccg ccagccacaa aaaagcgcaa agaagataaa
     7801 aaggtttgtt tctcatcacg cccccttaac gacattcagc tgatagctgg gttaataact
     7861 gctgctgact ctctggtggc agttgataat tggcgacaca gtgagcattt tcctcttctc
     7921 cccatttcac ctgcactttt cccgctaaag gcattccgct gaggtaaacc tgaccattat
     7981 ccgcaacaat gccgctactc tggctactct ctgatgtcac catcgcccca aacggcagcg
     8041 gcttattatt gtgggttagc gtcatgagca gttttatccc aacgcgcgct ttaaactctg
     8101 ctcgcacgat cgccccacgg gtgggaacaa cgttagcgac cgcattatct aaatcgacgt
     8161 tatcagccag ggtattggta tccagcgcca ctctgttttc ccgatattca gtggcataag
     8221 gcagcacggc ataaccccgc cagtcggtac gcactcctgt ctggttttcg acttttgcat
     8281 cttttgcgcc aggcgcttta acaagcacca ccgtatcgtt taacggctgc cccagcgtta
     8341 cgccattggc atgagccagt accccaccgc tgactccgta atagagctgc ttaatatcat
     8401 cgctatggct gtaaccgata ttggcattgc cgtaaccacc gcgataattc agcgtggcgt
     8461 agcctgtact tccgctattt ccatcgcctc ccccggcata accggtttgc acgctatagc
     8521 tgaggtcgtt gtcttccagc aacgtaccgt atataccggc cagattggtc atccgaccgt
     8581 tgagatcgtg tgacatgctg tagctggcac tggcatgtcg ccactgagat ttactgtcag
     8641 aacgcagcca gtggctgaaa ggaatattga cgttaagcgc taacatctga tcccgtcctt
     8701 tttgccaggc gtttttcgtc aggctatagc tgagcgtcca gttaatatct tcgaacgcag
     8761 tatttaatcc agcctggaat tgctcatcga cattactcgt tccccaataa gtttgatggc
     8821 taccactcaa atacagtgtt gatgtgcgcc cgagttgctg agtaacggtg agttgtaatt
     8881 tcccgcgttt gttataagcg agattgtaat agtcggtgaa tttcggctta acctgaataa
     8941 ctccatcctg tgtttcgatg ttgtagccat tcattcgact gtatgttgta tcagcgaaat
     9001 taaaataccc actggtcgaa taacggtaac ccactaactg aatattcgtg cctgactcat
     9061 tgagcgattt gttatagaga aaacgcaccg attgtccgtc atgctgactg tcatcgggaa
     9121 gtgtggaatt agcctgcgtc atatccacag acagagcgcc cagtgccccc atgtttttcc
     9181 cgataccgaa attaaaagca cgataacgat ctgccagttg cgttccacca tatattgtcc
     9241 agccagccgg aaggccgtgg agtaatgtac tctggaaaaa gcggggtttt tcctgttgcg
     9301 catttccact acggtattct cctgccgtaa tggaataacg agtatgccct tcacgttgca
     9361 aaagcgggac tgacgaatag ggtacggtaa aaatctgcgt gctgccgtca gcctctttaa
     9421 tcgttacctg caagtcacca ctattacctg cggcatagat atcgttgatg gtaaaaggcc
     9481 ccggtggcac cgtactatta taaatgtcat acccattttg tttaatagtg acctgtgcag
     9541 taccacgagc aataccgtgg atcaccgggg caaatcctct ttggctatcg ggtaacatat
     9601 tgtcatctga ggccaattgt gcgccgcgaa agttaatacc atcgaaaata tcgccctgag
     9661 tataaccatc acccagcgtc agccgggaac gtaacggtat tatgtctcgc tcaagccagg
     9721 tattgatatg ctgccattta tttttgctac ctgatgatct gtcgctactg ttataactcc
     9781 aggtggtatt gtcgcgtaaa cgccacgcac caatatttaa cccactctgt aggtttaaat
     9841 atgcataatg gctgttaccc ccaatccgat tctgtacact atttccgctg aaattataat
     9901 tgagcaatcc ggcattaata ccgggatccc ataactcagg aggaatataa ccacgcgcgc
     9961 gattactcat aaatgcctga gggatcgtca ggttgagtcg ctgctgacca acatctaaat
    10021 gcgcagtagc gtcatggatc atggcggtta atggcacgca ggcatcatcc gccagcagat
    10081 tcataccgga gatagaagcc gtattcagcc ccatactggc aagttgcgcg cgtgtcaggc
    10141 agggaacaat cccttgttcg ctgtcgcccg tattaaatgt gacatcacgc gttgccatat
    10201 aaccattatt caaatagata tcgacacgat acgtccctgg cggtaattct tgcccatttt
    10261 caaaacgcga taaatcggcc acagcctggg gatcatccgc taaaaagcgc ggattaaaat
    10321 agagttcggc agatgacaaa ggtgcctgtg cggcaaaagc acaggcgaca aagagccgga
    10381 caaaaaaacc agccaaacga tgcttacgaa tatgcaagca ttgtgtgttt cgctggtaaa
    10441 gtcttaaatt cagatatgac attattcctc cggtttaaaa tcacccggca gtcaattctt
    10501 tttattcagg cgaaaaaatc cccctgcgtt attccattac gcccgtcatt ttgggggtaa
    10561 gtgcgccata atcatttatt gttcggtaag taatattgct tcctgcatca gaaggcaatt
    10621 taaccgtgct ttcgcccatt ggaggcacca atgcattttc aagaacccgg gttccggcat
    10681 tcaactctgt taccgtcagg taatagggtg tcgggttgat cagcgtcaga gaattcgcgc
    10741 tacgacgaaa tcttaatttt tctgcggcct gatcgggtgg caacgctaat ttagccgggc
    10801 gatagtacag tttaatgcgg ctgataattg cgagctgtag cgtattctca gtcaattttg
    10861 atttatccat tgacggaatc gctttaacgt tcatccagaa taaactttcc cggtcctgtg
    10921 gcaattggtt atttgttgca tcaagaatac gcaaggtatt ctcttttttt cccttcatcg
    10981 caaacagagg aggcgtcacg ataaaacgac catcctttac accatcggca ttttccaccc
    11041 atgattgaat taaataggtg ctattttcat cattatttgt cacggcaagt tgcacttgtt
    11101 tttgccctgc cggataaatt acgcgagtcg cacctaaggc cactcccgct tcagcgcgtc
    11161 cggcaaccat cattgccatg aacatcagga tacctgccag caagcagaat gttatttcct
    11221 gcgatttcct tacattgacg tttttattac tcactgtcct gttcctgtta tcacattatc
    11281 tgctgaacaa ttactgatag gttaaagaga accaggcctg tgcattggcg atgccgccag
    11341 taacccgacg cccggtagca cgatatttgg cgatgaaatg tagcgaagta gagcctgagt
    11401 aaagccgttt ccagtttgct ggaggacgat taatcggtac gaggtttcct tcatcatcaa
    11461 acaacgctac gccaatattg gtggctatcc ctggcccctc tcccacggaa agcacatccg
    11521 gatttttacc atccgcgaca ccgtgaaacg ccacacccac acgttcactc accaccgtgc
    11581 tacattcccg taaatgaata acaaaaggca ccggtgcgct atcttcccct accgcgtgaa
    11641 accggttact gctgatttgc cccatattga ccgtcatttg tttatcacca gcttcaatcc
    11701 ggcaagtttc cgcaataatg acgccctgaa attgcatatt tccgccgggc agcgtggtat
    11761 tccatttatt tccggccaga gcaaacattg gcagcaacga cgctaataga aataatctct
    11821 tccttttcat cgttattttt atcacacaag ggtgggcatc cctgcccgta atgacgtccc
    11881 tgaacctggg taggttattg atactgaacc ttgaaggtcg catccgcatt agcagcaccc
    11941 ggggttgcgg caccggttgc aaaataacgc gcctggaacg gaatggtgtt ggttccgtta
    12001 ttcagggttg tttctgaact aaatgtcgca ccgtccagcg ccagcgcagc acccgttctg
    12061 tccaggatct gcacaccaac gtttgttgcg ctacccgcag ctgaactctg cagagccagt
    12121 acatcagtat gcgtagcatc aattgccgta cctaagaagg caacagcggc ttttgtggca
    12181 acagtggtat cgcaatcatt cagctgaatg ttaaaaccaa cggcagagct ggttgctcca
    12241 gcctgcttca ggctagcggt acgaacctgg cctaactgaa cggtttgatc aacagagcct
    12301 gcatcaactg cgcaagcggc gttaacaact tcccctttaa agtgaacggt cccaccattt
    12361 accgtcgtag tatcggccag agccgctgcg gaactgaggg acagagccga cagaacaacg
    12421 attgccagag ttttaatttt catgctgctt tcctttaaaa aactatttct aaatcgacat
    12481 gggcagtcgt tctgtacact ttgttttgtc aacgagtttg actgccaaca ctgcacagtt
    12541 tt
//
LOCUS       DAHHTG010000121        12423 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.122, whole genome shotgun sequence.
ACCESSION   DAHHTG010000121 DAHHTG010000000
VERSION     DAHHTG010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12423)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12423)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12423
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.122"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            136..528
                     /locus_tag="NBI74_003580"
     CDS             136..528
                     /locus_tag="NBI74_003580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001357433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2787 family protein"
                     /protein_id="HCD1933860.1"
                     /translation="MPVDQELINIILNEAGNPPPHKAKITAVSLLFKDLSYSAEKGGY
                     HPVEIRIISRNDEWYFDYITDFSYMGTVYPELEKEIDISWSQQYVFLAYVGDLPVNAG
                     RELFELWQSNFIQYHGMNIYTITVLWES"
     gene            648..1109
                     /locus_tag="NBI74_003581"
     CDS             648..1109
                     /locus_tag="NBI74_003581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000954131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1933861.1"
                     /translation="MLSWLYDGRVKRRPLMNWLIQTYQQRWPLHEWLTEGIEEDRLDW
                     LIAQVLQKGHYRRQFPVEITRPFAGKRGLTDGRLFREMQRFLDVTDHSRLIMLSDQFH
                     WSLLVKMDEEMLCFFDSNGRTTMPRKAFSLRTGVTRRQLFPDAIYFIEREF"
     gene            1111..1563
                     /gene="radC"
                     /locus_tag="NBI74_003582"
     CDS             1111..1563
                     /gene="radC"
                     /locus_tag="NBI74_003582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000177771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HCD1933862.1"
                     /translation="MTMFTQRETRILDQARDIISRYYQRGVQLCSPDDVRRCVMVELA
                     PLEHEEFGIILLDNQNQLLHREILFRGTLNSVSVHPREVIKRVLKHNAAAVILVHNHP
                     SGEPEPSRCDIQLTKKLQELLEILDVRLLDHFIVAGTETVSMAERGLV"
     gene            1610..2539
                     /locus_tag="NBI74_003583"
     CDS             1610..2539
                     /locus_tag="NBI74_003583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019083242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCD1933863.1"
                     /translation="MSRTALRQVVPVVQTTVFDAYIVGLAPSGRRGITSLLNRSASIL
                     KHGADAADYPWEQLNYAAVAKVRAALLDDGYAVSSVNMALSALRGVAQTAFNLNYMDA
                     ETLARIRSVKRVSGDVQRKGRALGRQEIRVLIQAAKQHPQSVRRCRDVAIVLMLCGTG
                     LRAGELVKLERRDYDNGILTVRQGKGRKYREIHVADAVDKAIRAWLKVGADEADSALF
                     RRIQRSGKVASQPLTTTGLTGILAELQQTAGIARFTPHDMRRTFITRLLEQGVDINTV
                     RQLAGHSDISTTTRYDCRGDTMKISASKRIRCF"
     gene            complement(2526..3140)
                     /locus_tag="NBI74_003584"
     CDS             complement(2526..3140)
                     /locus_tag="NBI74_003584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000580009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1933864.1"
                     /translation="MIGNQSNKADARCHRERKSSNEVFEFLEKKLYLGRDTRQKLALI
                     ARKLTGAGFSPDKFAAEKAADVLSACVNHMFNDLLNEGEPNGIYRPEEHPMIVPARSP
                     KALEIYTVYQLVKGRFDKLHTGESDREKYASVAEKLNEYGIWKPRLKRLSDGKEWARE
                     DVAWMLQPENINAQINIQNQKYARLSAEDETETESGNASTAKNI"
     gene            3363..3560
                     /locus_tag="NBI74_003585"
     CDS             3363..3560
                     /locus_tag="NBI74_003585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016248781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family transcriptional regulator"
                     /protein_id="HCD1933865.1"
                     /translation="MNTNRILRKKEVLHLTGISSATLYRLISKGFFPLSKRLTGDNGR
                     AVGWLESDINNWVNSRMQAGE"
     gene            3562..4029
                     /locus_tag="NBI74_003586"
     CDS             3562..4029
                     /locus_tag="NBI74_003586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000658784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1933866.1"
                     /translation="MKEIKSKTSSAKKTLVPWFETMGKQVGLSQETISAVLKEQRLRI
                     RKLNYASAYTVSNILIQDCGGADDMWSNYAKLALILERCKYIPCGSRACAYCSTAQQL
                     HYQGVDVTGEYALYQLPELFLDFLETNDKNSSYHWDYNKLVERHEDAQARSFG"
     gene            4117..4971
                     /locus_tag="NBI74_003587"
     CDS             4117..4971
                     /locus_tag="NBI74_003587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001000136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1933867.1"
                     /translation="MNDSYYFRTSDPDYIRHAPSMLEILSKAFPTIITQQESEYELQR
                     LNHLLNKLKGSNRCYNIIAQKLRQCRNGSPCNSLICPHCQRERILAQLAMLHVLPGNS
                     AEYVGVVLFFNKDTQTPPPWKNIGALRAQIGRYKQRISRVLNRLGYAGPATGTFSMMR
                     HMPDGPEERIFWVPQLCLFLPNDSTLIKGLKAHMSRSGGAFIDASTLNTPVIVLRYKD
                     PARLISCALNPVWHTADYTLTDKDALVKSRMELLKGRTLLKSLLTLDSLGTGVVSFSF
                     GQPPGKVH"
     gene            5572..6945
                     /locus_tag="NBI74_003588"
     CDS             5572..6945
                     /locus_tag="NBI74_003588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000701230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1933868.1"
                     /translation="MKIPKWVYDRITEITDYVVGDTQWAVTRRQTVRWFLAHIWLETD
                     DDGWTICTVRDIRSSYASLLGQCEISYQGECFMSTLTDFLPTLSDIEFRAGKASKDPE
                     KRRASAWRFNPLLPVCDESARGKLNLVDLDTGESVSFRALLQGNGKAPGHAIDVEKRQ
                     TALKQTEKDFLAKVGRGRMSIQFVKELRSREPDHYYRAGIRSLNHLYNGRIEGQYVTY
                     DHHYRLTFGGRYYDQAFQNLPNEFKAKFRTGLLNYDIEACNLACLNHLFRQYDVDYRV
                     KSSIYKTMMEHTGLTRKQCKQMVHTTTYRIGRVTIGVNDGLGAKVYGWCGNSKKKALK
                     ILRWWNRYISPLKSALESLLERVHGAHRKSCSSPRNYHRYANEVGLILDLNSEVYQRE
                     KTHHHQYARNKALLAFMICGVEQAYIREVVSMNPGRVCMLDHDGVVATGALSLPDWRG
                     FTMKVKD"
     gene            7123..7746
                     /locus_tag="NBI74_003589"
                     /pseudo
     CDS             7123..7746
                     /locus_tag="NBI74_003589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000830285.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            7941..10598
                     /locus_tag="NBI74_003590"
     CDS             7941..10598
                     /locus_tag="NBI74_003590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006809919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2339 domain-containing protein"
                     /protein_id="HCD1933869.1"
                     /translation="MDELLTLGCIALFFALVVVPILAIIALSRSTAVRSELATLRRRV
                     EVLEQRGVTEAPATVVTTVPQSVSQMVVPVETSSPAVNIVLSEPVADVTPESVDPWRP
                     QPKPQNEALPTPAVEQSSAFGGVMSSLVRWFMQGNPLAKLGILLLFLGFSFLLRYTVE
                     HSLFPLELRLVATSLFAIALLVVGWRLRHKQLVYALIIQGGATGTLYLTVFGAFWLWQ
                     MLPMTLAFALLVAICAASVGLAIMQKALSLAMLASIGGYLAPLLLSTGGGSHVALFSF
                     YLLLSVGILAISIWQHWRELNLLGLLFTFGIGGLWGLDGYRSEYYLSSQLFLIANTII
                     FGVLSVALSLRAQEKGKQIIDGVLLFASPLVGFGMQYAITRHMEYGPALSALGYGGFY
                     LALAWLALRRYPSLGRPLVLAALALGGAFTTLAIPLALSARWTAMAWALEGLGILWLG
                     VQQQQRRMSYSGTALLVLAVCSALWAQMNGMSALSLVLIFAVLSLSWLAAAWLWRNIQ
                     LQGSWVLLAGGLIFWIIALIGASQLVLKKDSLVLSGVLALMAISVWGWRIVSGRLAWW
                     ELDVSKWLLWPTMLVMLLSQISQHEIFAAGWQNLAWCLALPAAGALLWRDAETLPPRL
                     SRLAHLSLFWMILLALAAELFWFAQDLPWGMAAWGSGLMMAAGGLLIFLVHEAVHRQL
                     WPFRSWPALYASQAMIPVAAVLVVLLVLTNLQDGVVYRQTWLPLVNPLEEGAAFALLG
                     LIVFYRVSQRFFPVQISVCRPWPLIALLALSFWWLNGILLRALAWYGEVPWSAETLWH
                     SRLIQTCFALFWMLAALVVMLRATRRCSRREWLCGSVLLGIVIVKLMLVDSARGGGLA
                     RAVTFIGVAILVLIVGYFSPLPPKAGEEK"
     gene            10595..11990
                     /locus_tag="NBI74_003591"
                     /pseudo
     CDS             10595..11990
                     /locus_tag="NBI74_003591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017383163.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3999 domain-containing protein"
     gene            complement(12177..12314)
                     /locus_tag="NBI74_003592"
     CDS             complement(12177..12314)
                     /locus_tag="NBI74_003592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1933870.1"
                     /translation="MGQHGKSLPSFDAPETLKGRKIEWEYENLTHGNKAYNGYVTAIL
                     N"
BASE COUNT         2885 a         2929 c         3455 g         3154 t
ORIGIN      
        1 gtcagtacgt tgtcatgcac ttaccattta ttaggtgatt aaaccccacc tgtgctggtg
       61 cagacataac gtcttatcag aatatattga acaggaaaat cactgatgaa tattgtccat
      121 caggaagggc tagctatgcc cgtcgatcag gaattaataa atatcatatt aaatgaagcg
      181 ggaaatccac cgccgcataa agccaaaatt acagccgtca gtctgttatt taaagacctg
      241 agttacagcg ctgaaaaggg tggttaccat cctgtcgaaa tacggattat ttcacgcaat
      301 gatgaatggt attttgatta cattaccgac ttcagttata tggggacggt ataccccgaa
      361 cttgaaaagg aaattgatat cagctggagt cagcaatatg tctttcttgc ttatgttggt
      421 gacctgcctg ttaacgccgg aagggagctg tttgaactct ggcaatccaa ctttattcag
      481 tatcacggca tgaacatcta caccattacc gttctctggg aaagctaatc cccctgtact
      541 aaccatcact aatttatggg gaattatctg tgacgactga cagagggata cgtgcgtctc
      601 aacagggcga actggactgt ctttgcggtg cttattgcgt ggttaacatg ctgagctggc
      661 tgtatgacgg cagggtgaaa cgacgcccgt taatgaactg gcttattcag acttatcagc
      721 agcgctggcc tttgcatgaa tggctgactg aaggaattga agaagaccgc ttagactggt
      781 taatagctca ggtgttgcag aaagggcatt accgccgcca gttcccggtt gaaatcacca
      841 gaccctttgc agggaagcgg gggttaacag acgggcggct gttcagggaa atgcagcggt
      901 ttcttgacgt cactgaccac tctcgcctga ttatgctcag cgaccagttt cactggtccc
      961 ttctggtcaa aatggacgaa gaaatgctct gcttcttcga cagcaacggg cgaaccacca
     1021 tgccccgtaa agctttctcc ctgcgtactg gcgtaacccg ccgccagctg tttccagacg
     1081 ccatttactt cattgaacgg gagttctgac atgaccatgt ttacccagcg tgaaacccgt
     1141 atccttgacc aggcgcgtga cattattagc cggtactacc agcgtggggt tcaactctgt
     1201 tcccctgatg atgttcgacg gtgcgtgatg gttgagctgg caccgcttga acatgaagag
     1261 tttggcatca tcctactcga taatcagaat cagctgctgc accgcgaaat cctgttcagg
     1321 ggtacgctaa actcggtcag cgttcacccc cgtgaagtta ttaagcgtgt gctgaaacat
     1381 aacgccgccg ccgtcatcct tgtccataat caccccagcg gcgagcctga acccagccgg
     1441 tgtgacatcc agttgaccaa aaagcttcag gagctgctgg aaatactgga tgtgcggtta
     1501 cttgatcact ttatcgtggc aggaactgaa acggtgtcga tggctgagcg aggactggtg
     1561 tgatgctgaa agttacttgg tttaatgtac gttattgcgg gttacgcgca tgagcaggac
     1621 agcgttgcgt caggtcgtgc cggtggtgca aaccaccgtg ttcgatgcgt atattgtcgg
     1681 tctggccccc tcgggacggc gaggtatcac cagcctgtta aaccgcagcg ccagcatact
     1741 taaacacggt gcggatgcag cagactatcc gtgggaacaa cttaactacg ccgccgttgc
     1801 caaagtgcgc gcggcactgc tggatgacgg ttacgccgtg tcatcggtca acatggcgct
     1861 gtcagcactc cggggcgtag cgcagacggc ctttaacctc aattacatgg atgccgaaac
     1921 gctggcacgt atccggtcag ttaagcgcgt gagcggcgat gtccagcgca agggaagggc
     1981 gctgggcagg caggaaatcc gggtactgat tcaggctgca aaacagcatc cgcaatctgt
     2041 acggcgctgt cgcgatgtcg ccattgtgct gatgctttgc ggaacgggcc tgcgggccgg
     2101 ggaactggtt aaacttgagc gccgggacta tgacaacggc atcctgacgg tgaggcaagg
     2161 gaaggggcgt aaataccgtg aaattcacgt ggcggatgcg gtggacaagg ctattcgtgc
     2221 gtggctgaaa gttggcgcag acgaggctga cagtgcgttg tttaggcgaa ttcagcgcag
     2281 tggtaaggtc gcaagccaac cgctgactac gaccgggtta acgggtatcc tggctgagtt
     2341 acagcaaacg gcaggtatag cacgttttac cccgcatgat atgcggcgca ccttcatcac
     2401 ccgtttgctg gagcagggcg tggatatcaa taccgtccgc cagttggcag gccacagcga
     2461 catatccacg acgacgcgct atgactgtcg tggtgatact atgaagatca gtgccagcaa
     2521 gcgcatcaga tgtttttagc agttgaggcg ttgccagact cggtttcagt ttcatcttca
     2581 gcactcaacc gcgcgtactt ttgattctgg atgttaattt gtgcattgat attttcaggc
     2641 tgaagcatcc acgcaacatc ctcacgcgcc cattctttac cgtcagagag tcgttttaac
     2701 cggggtttcc atattccata ctcattaagt ttttctgcta ccgaagcata tttctcacga
     2761 tctgactccc ccgtatgaag cttgtcaaaa cgacctttta caagctggta aacagtatag
     2821 atttccagtg ccttagggga cctggccggg actatcatgg ggtgttcctc cggcctataa
     2881 atcccgtttg gctctccctc attgagcagg tcattaaaca tgtggttcac gcaggcactc
     2941 agcacatctg ccgctttctc cgcagcgaat ttatccgggc taaaacccgc tcccgtcagt
     3001 ttccgggcaa tcagtgccag tttttgccgg gtatcccgtc ccagataaag cttcttctcc
     3061 agaaattcaa agacctcgtt actgcttttc cgttcccggt ggcatctggc gtcagccttg
     3121 ttgctttggt taccaatcat agtcacctcc atgacagtgt tttgtacaaa atatcaccat
     3181 tgagcctcag tgaaaattgc ttctttatat gaccgttctg gagacagaaa tagatgatct
     3241 cgaatgacat ccacattaaa gatgccctta acgtgctcaa cgaaggcatc gttgagtgaa
     3301 tccttgcgtt ttttggtggt tgtaaataac ccgctcctga aacagctcaa aggagcacaa
     3361 gcatgaacac caaccgtatt ttgcgtaaaa aagaggtact tcatctgacg gggatctcgt
     3421 cagcgacgtt gtaccgactc atctcgaaag gattctttcc tttgtcaaag aggctaacgg
     3481 gcgacaatgg ccgggcggta ggctggctgg agagtgatat caataactgg gttaacagcc
     3541 gtatgcaggc gggagaatga aatgaaagag ataaaatcga aaaccagttc tgcgaaaaaa
     3601 acgctggttc cttggttcga aactatgggg aaacaggttg gtttatcaca agaaacgata
     3661 tcagcagtgt taaaggagca gcgtttacgg atacgtaagc ttaactacgc atctgcatat
     3721 acagtgtcca atattctcat acaggattgc ggcggggccg atgacatgtg gagcaactac
     3781 gctaaactgg cgcttatcct tgagcgttgt aaatacattc cctgcgggag cagggcctgt
     3841 gcatattgtt ctaccgctca gcaattacat tatcaggggg tggatgtaac cggggaatac
     3901 gctttatatc agttgcctga gttattttta gattttttag aaacaaacga caaaaattcc
     3961 tcttatcatt gggattacaa taagttagtt gagcggcatg aagatgctca ggcgaggtca
     4021 ttcgggtaat agccatttct gtatggtgaa aaactgacat attaccctgc cactgcattt
     4081 tggtggcagg gtaatcaata cagctaggaa tattaaatga acgattctta ttacttcaga
     4141 acatctgacc cggattacat tcgtcacgca ccgtcaatgc ttgagatttt aagtaaagca
     4201 ttccccacta ttattaccca gcaggaaagt gagtatgaac tgcagcgtct taaccattta
     4261 ctaaataaac tgaaaggcag caatcgctgc tacaacatca ttgctcaaaa actaaggcag
     4321 tgcagaaacg gatctccatg caacagtctt atatgcccgc attgccagcg ggaacgcatt
     4381 ctggcgcaac tggctatgtt gcatgtcttg ccggggaatt cagcagaata cgtgggagtg
     4441 gtcctgtttt tcaacaagga tacgcaaact ccgccgccat ggaagaacat tggggcgctc
     4501 agggcgcaaa tcggtcgtta taagcagagg ataagccggg tacttaaccg cctggggtac
     4561 gccggtccgg ccacaggcac cttcagtatg atgcgacata tgcctgatgg cccagaagag
     4621 cgtattttct gggttccaca gttgtgtttg ttcctgccca atgacagtac gttgattaag
     4681 gggctaaaag cgcatatgtc gcgaagtggt ggggcattca ttgatgcatc gacgcttaac
     4741 acaccagtga tcgtgctcag gtataaagat cccgcgagac tgatttcatg cgccctgaat
     4801 ccagtctggc acacagcaga ttacacactg actgataaag acgctcttgt taagtcaaga
     4861 atggagctac tgaaaggccg gacactgtta aaaagtctgc ttaccctgga ctcactcgga
     4921 acaggtgtcg tttctttttc gtttgggcaa cccccaggga aagtgcactg atttaccggt
     4981 aatgcagcaa ctgcaatgca cttttgctgt tgattaaatt gcagtggaac gacgaataac
     5041 agcggcaacc actgcatttt cgttgttgta aaactgcaaa gcttgccagt gattttgatg
     5101 gcgctgatgc actttaacat tccaactgtc tcgattttaa cgcaaccgtc tgttttctgc
     5161 aggggctcac ggatgcctct gcacacccga tccttaccat caactgacga gttagcgcat
     5221 ctgtgagggc tttctgtgtt tctgtcagtg aatattctac acacaatggg gggagcggtc
     5281 agcgagcacc attgccattg agcgcaacgg agagcttggg gacatgcagc actacggagg
     5341 cttcatctcc ttctcatgtc attaaacgat acggcgaacg agcgcagcga gtgagggaag
     5401 gcgtcagcct gaacaggggg aaagatttgc gaccggagtg gagcaaatca tgggcggagc
     5461 ccatcctcgc gactggggtt ttgactttcg aattgcccag cgttgagttt ggttaatgta
     5521 gtgagacgag gatgctgaga ggggaccgtg agtactgaaa aggattatcc agtgaagatc
     5581 cctaagtggg tttatgaccg tattactgaa ataacggatt atgtcgtggg ggatacgcaa
     5641 tgggctgtta cccggcggca gacagtacgg tggttcctgg ctcacatatg gctggaaaca
     5701 gacgatgatg gctggactat atgcactgtc cgggatatcc gtagcagcta tgccagtctc
     5761 ctgggacagt gtgaaattag ttaccagggg gagtgcttta tgtccacgct aactgatttc
     5821 ctgccgacac tttcggatat tgaatttcgt gcaggaaaag cgagcaaaga cccggaaaag
     5881 cgtagagcca gtgcatggcg gttcaacccc ctgctgcctg tgtgtgatga gtctgccagg
     5941 ggtaagttaa atctggttga cttggatact ggtgagtctg ttagttttag agctctgctt
     6001 caggggaacg ggaaagctcc tgggcatgcg atagatgtag agaaacgcca gacggcccta
     6061 aaacaaacgg aaaaggactt cttggccaaa gttgggcggg ggcgcatgag tatacagttt
     6121 gtgaaggaat tgcgtagccg tgagccggat cactattacc gcgcaggaat acgcagcctt
     6181 aaccatctct acaatggcag gattgaggga caatatgtca cttacgacca tcattatcgt
     6241 ctgacgtttg gagggcggta ttatgatcaa gctttccaga atctgcccaa tgagttcaaa
     6301 gctaaatttc ggaccgggtt actcaactat gacatcgaag cgtgcaatct ggcctgtctg
     6361 aaccatctgt tcagacagta tgacgtcgat tatcgtgtga agtcatcgat atataaaacc
     6421 atgatggaac ataccgggct gacccgtaaa cagtgcaaac agatggtcca taccaccacc
     6481 tacagaattg ggcgtgtgac tattggtgtt aatgatggcc ttggggcaaa agtgtatgga
     6541 tggtgcggta acagcaaaaa aaaggcgcta aagattttgc gttggtggaa ccgttatatc
     6601 agtccattga aatctgcgct tgagtcattg ctggaacgtg tgcatggcgc acaccgcaag
     6661 agttgttcat caccgcgtaa ctatcacagg tacgctaatg aagtcggatt aatactggat
     6721 cttaatagtg aggtgtatca gcgtgaaaag actcaccatc atcaatatgc gcggaataag
     6781 gcactgttgg cgtttatgat ctgtggtgtg gagcaggcgt atatccgtga ggttgtcagt
     6841 atgaaccctg gtcgtgtctg catgctggat catgatgggg tagtggcaac gggcgcgctt
     6901 tcactaccgg actggcgtgg tttcacgatg aaagtgaagg actagcttac tttcttgaat
     6961 ttaactatct gcgatctctg ataagggcgt attacgttgt caatcataat gggatatgcg
     7021 cattggaacg atgtgcatat ccagtaagtg atccatgtcc gctttgagct gtgagttcaa
     7081 cgggtcgatg caacgctgtc cctaatcaag ggggacgttg ccatgaaacg aagaactcgt
     7141 attaactaca cgccagagca aaaggtgatt atttgggaca gatataagca aggtgattcc
     7201 ctgcatgata tccccagaat gttcgacaga tttcactcta ctatcatgcc tacaatccac
     7261 caggccggtg gctaccgtcc tcccgtgcga aagcggcacc ggttagcgtt ttcgcttgat
     7321 gaaaaagagg agatatccag aggactggta gcaaaacgca tcatcaggga catcgctgct
     7381 aaattatcaa tagctccctc aacgattagc cgcgagatca ggaggcacgg cggtgcaaaa
     7441 caataccgtg cagcaaaagc cgatcctgct gcgtgggaaa atgctctgag accaaaacct
     7501 tgcaagctaa ttgaaagccc cacattgtgt aaaatcattg cagagaagat gcatcaggac
     7561 tggtcgccgg aacaaattgg cggttggcta aaacgctgtt atcccgataa tcaggaaatg
     7621 catgtgtcac atgaaatgat ttataaaacg ctttttatac caacccgggg agctttaaaa
     7681 aaagagctgc agcaatgcct cagaagcgga agagtagtgc gtagatccaa aacctcatca
     7741 cttaaagtaa acagagatac attctggtca cgaatgcccg gttgttcgtg ctttgcgttt
     7801 taatcgcatt ttacagtttc tgcctgcgtg cagaggttga tggtattttc tgaaaaagcg
     7861 gctcgctttg ctaatgcata tggcacaaat acaacagttt gtgtcattct gacccatctt
     7921 tataccgcga cggtggaaac atggatgagc tgctaaccct tggctgcatt gctttattct
     7981 tcgcactggt ggtggttccc atcctcgcga ttatcgcgct cagccgcagc acggctgttc
     8041 gttctgagct ggcgaccctg cgtcgccggg tggaggtgct tgagcagcgc ggtgtgactg
     8101 aagcacccgc cacagtggta acaactgtgc cgcagagtgt ttcgcagatg gttgtgcctg
     8161 ttgaaacgtc ttcgcctgcg gttaacatcg tgctgtcgga gcctgtggct gatgtcacgc
     8221 ctgagtccgt tgacccctgg cgtccacaac cgaagccgca aaacgaggcg ctacccacac
     8281 cagcggtcga acaatcttcc gcttttggtg gggtgatgtc gtccctggtg cgctggttta
     8341 tgcagggcaa cccgctggca aagctgggta ttctgcttct cttccttggc ttctccttcc
     8401 tgctgcgtta tacggttgaa cactccctgt tcccgcttga gctacgtctg gtggcgactt
     8461 cgctgtttgc gattgccctg ctggtagtgg gctggcgatt gcggcataaa cagctggttt
     8521 atgcgctgat catacagggt ggagctacgg ggacgctcta tctcaccgtt tttggcgcat
     8581 tctggctctg gcagatgctg ccgatgacgc tggcgtttgc attactggtg gcgatttgcg
     8641 cggcgagcgt gggactggcg attatgcaaa aagcgctcag cctcgcgatg ctggcaagta
     8701 tcggtggcta tctggcaccg ctgttgcttt ccaccggcgg cggtagtcat gtggcgctct
     8761 tctctttcta ccttctgctt tccgtcggta ttctcgccat cagcatctgg caacactggc
     8821 gcgagcttaa tctgcttggc ctgttattta cctttggtat cggcggtctt tggggactcg
     8881 acggctatcg atcagagtat tacctgagca gtcagctttt cctgatagcc aatacgatca
     8941 tttttggtgt gctgagcgtg gcgctatcgc tacgagcaca ggagaaaggt aagcagatta
     9001 ttgatggcgt gctgttgttt gcatcgccgc tggtcggttt tggcatgcag tacgccataa
     9061 cccggcatat ggaatatggt ccggcgctta gcgcgctggg ttacggcggt ttctatctgg
     9121 cgttagcatg gcttgcattg cgtcgctatc cctctttggg gaggccgctg gtgctggcgg
     9181 cgctggcgct gggtggggca tttaccacgc tcgctatccc actggcgctt tcggcccgct
     9241 ggacggcgat ggcctgggcg ctggaaggtc tcggcatcct ctggctgggc gtgcaacagc
     9301 agcagcggcg tatgagctat agcggcactg cgctgctggt gctggcagtg tgcagcgcgc
     9361 tgtgggcgca gatgaacggg atgagcgcgc tttctctggt gctaatcttt gccgtactca
     9421 gcctcagctg gctggctgcc gcctggctgt ggcgcaatat tcagttgcag ggtagttggg
     9481 ttctgttggc tggcggtttg atcttctgga taatcgcgct cattggggca tcgcaactgg
     9541 tgctgaaaaa ggactcgctg gtgctgtctg gcgtgctggc gttaatggcg atatcggtct
     9601 ggggctggcg gattgtctct ggtcgtctgg cctggtggga gctggatgtc agcaaatggt
     9661 tgctgtggcc gacgatgctg gtgatgctgc tgtctcaaat ttcacaacat gagatttttg
     9721 ctgcgggctg gcagaatctg gcctggtgtc tggcgttacc cgctgcgggg gcattgctct
     9781 ggcgtgatgc tgagacgttg ccgccgcgcc tctccagact ggcgcacctt tcactcttct
     9841 ggatgattct gctcgcgctg gcggcggagc tattctggtt tgcgcaagat ctgccatggg
     9901 gcatggcggc gtggggctcc ggcctgatga tggccgcggg gggcctgctc atctttctcg
     9961 ttcatgaggc ggttcatcgt cagctatggc cattccgaag ctggcccgca ctctacgctt
    10021 ctcaggcgat gataccggta gctgcggtat tggttgttct gctagtactg acgaatttgc
    10081 aggatggcgt agtttatcgc cagacctggc tgccgctggt gaatccactg gaggaggggg
    10141 ccgcttttgc gctactggga ctcattgtat tttatcgcgt ttcgcagcgc tttttcccgg
    10201 tgcagatttc ggtatgccgt ccctggccgc tcattgcact gctggcgctt agcttctggt
    10261 ggctgaatgg catcctgctg cgcgcattgg cctggtacgg cgaagtgccc tggagcgcgg
    10321 aaacactgtg gcattcgcgg ctgattcaga cctgttttgc cctgttctgg atgctggcgg
    10381 cattagtggt gatgctacgt gcgacccggc gttgctcacg tcgcgaatgg ctttgtggat
    10441 ccgtattgtt agggattgtt attgtgaaac tgatgttggt agacagtgcg cgtggcggcg
    10501 gcctggcacg cgcggtcacg tttatcggcg tggcaattct ggtgctaatc gtcgggtatt
    10561 tttcaccgtt accgcccaaa gcgggagaag aaaaatgaaa tggatgaaag cggtgttatg
    10621 cagcgtgcta ctgagtactg cgggcgcggc ggtcagcagc gatgaagtga aggaagcgcc
    10681 aacggactac gcaacaggcg tcgcgctgga aaccacgggg gcattcgccc tggtatcgca
    10741 tttcgctgcc gcaggtggtg tatcagagca ctgcatggcc ggatttgcgc gatgtgcggg
    10801 tttttaacca tctgggggat atggtgccgt ttgcgctggt ggcgcagaaa acccagaccg
    10861 ttacgccaga gacagtggct ctgcgtcttt tcccgcttga tatgtcgccg gttccgccgc
    10921 gtgatgaggg tcaacgtagt ggggatgctg ttgttttacg ttcaaaaaac ggtattgaaa
    10981 ttcatttgca aagcgatgat gtcactgcgc tcgggcagag ctatttactg acgttgccgg
    11041 aagagaggaa agactctctt tcactgacgc aattgcgtct gaactggggg acgccgacgg
    11101 gcaactggca ggggaaagcc tcggtttatg tcagccgtga tttacgctac tggcgacccg
    11161 tgcaggaaga tgcgccgtta atggatctga cgcgggatag cgatcgtctg aaaatggacg
    11221 ccatcagcac caacctgaca ttgtcacttg aaggaaatcg ctacctgctg gtgatcctca
    11281 actcgcaaag cccggcactg acgttaaaca gcgtgagcgc tattgctgac agtaatgagc
    11341 cggagtccga acgtattgtg attggcgctc gggcggataa ggtgtcggat gatgaagccg
    11401 tctggcgatg gacacagccg cagcccctga catcgttgcg gatcgatctg gaaaatgaag
    11461 gcgttttacc ggtggaactg gtctggcgca gcggcgaaaa agaaccctgg cagtcactga
    11521 caaaaacggt gttgtatcgc ctggacggga agcgttcaga agatattcgt cttcctggcc
    11581 agttagttga ggctgttcga atacgtacaa ttaatgcgcg attgccggag gcattgccag
    11641 cattgagcgg tgcgcgcgac agttatcaac tggtttttaa cacgcaaggc aaaggtcctt
    11701 acatgctggc gtggggaaac cgggcggcga aaaaggccga tgttggactc gatatgttga
    11761 tccctgcgtc gctgcgtaaa acgcaggaga tagataatct gccgtgggct ataccgcagg
    11821 agagcgtaac tctcggcggt gaattgcgac taacggcgac gtcggcagcc gaacaacaaa
    11881 gtcagtggaa aacgctgctg gtgtgggggg cgttaatact tggcgttgcc gttctggcgt
    11941 ttatggcatg gcgaatctgg cgagaagtta aaaaggacgg tgcggcgtaa gccataaaaa
    12001 agcacagttg catagttcac ggtgttgata aactgttaca acagaggcgt ttaagctgag
    12061 gcggggaaat aatctatttg tgaataatat gttacttcac tggggcgtga gtcatgggaa
    12121 tgattttgat acctgcgttt tttattttag ccatcctgat tatcgctatc ttcatttcag
    12181 ttaagaatgg ccgtcacgta tccgttgtat gccttgttgc cgtgggtgag attttcgtat
    12241 tcccattcta ttttacggcc cttaagcgtt tccggagcat caaaacttgg taaagattta
    12301 ccatgttgac ccattatgcg gcccctctgg agaccacttt caggtatttt ctctactgat
    12361 atgtacacat ggagagatgt gattgcaaat tcccatcagc attactacca aaagaatcag
    12421 aca
//
LOCUS       DAHHTG010000122        12106 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.123, whole genome shotgun sequence.
ACCESSION   DAHHTG010000122 DAHHTG010000000
VERSION     DAHHTG010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12106)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12106)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12106
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.123"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            <1..94
                     /locus_tag="NBI74_003593"
     CDS             <1..94
                     /locus_tag="NBI74_003593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311911.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HCD1933871.1"
                     /translation="FHNDSTPRYDKSAADLAWQRTLKWFDKYLS"
     gene            240..1472
                     /gene="mdtM"
                     /locus_tag="NBI74_003594"
     CDS             240..1472
                     /gene="mdtM"
                     /locus_tag="NBI74_003594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001420685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtM"
                     /protein_id="HCD1933872.1"
                     /translation="MAGLKATHAATLFFPMALILYDFAAYLSTDLIQPGIINVVRDFN
                     ADVSLAPAAVSLYLAGGMALQWLLGPLSDRIGRRPVLITGALIFTLACAATMFTTSMT
                     QFLIARAIQGTSICFIATVGYVTVQEAFGQTKGIRLMAIITSIVLIAPIIGPLSGAAL
                     MHFVHWKVLFAIIAIMGFISFVGLLLAMPETVKRGAVSFSAKSVLRDFRNVFCNRQFL
                     FGAATISLSYIPMMSWVAVSPVILIDAGGLTTSQFAWTQVPVFGAVIVANAIVARFVK
                     DPTEPRFIWRAVPIQLVGLALLIIGNLLSPHVWLWSVLGTSLYAFGIGLIFPTLFRFT
                     LFSNNLPKGTVSASLNMVILMVMSVSVEIGRWLWFNGGRLPFHLLAVVAGVIVVFTLA
                     GLLNRVRQHQAAELAEEQ"
     gene            1513..2793
                     /locus_tag="NBI74_003595"
     CDS             1513..2793
                     /locus_tag="NBI74_003595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF445 domain-containing protein"
                     /protein_id="HCD1933873.1"
                     /translation="MNKLIELRRAKMLALSLLLIAAATFVVTLFLPPNFWVSGVKAIA
                     EAAMVGALADWFAVVALFRRVPIPIISRHTAIIPRNKDRIGENLGQFVQEKFLDTQSL
                     VALIRRHEPALLIGNWFSQPENARRVGQHLLQIMSGFLELTDDARIQRLLKRAVHRAI
                     DKVDLSGTSALMLESMTKNDRHQVLLDTLIAQLIALLQRDKSRKFIAQQIVRWLESEH
                     PLKAKILPTEWLGEHSAELVSDAVNSLLDDISRDRAHQIRHAFDRATFALIDKLKNDP
                     EMAARADAVKSYLKEDEDFNRYLSELWGDLREWLKVDINSEDSRVKERIARAGQWFGE
                     TLIADDALRASLNGHLEQAAHRVAPEFSAFLTRHISDTVKSWDARDMSRQIELNIGKD
                     LQFIRVNGTLVGGCIGLILYLLSQLPALFPLGNF"
     gene            2909..4060
                     /locus_tag="NBI74_003596"
     CDS             2909..4060
                     /locus_tag="NBI74_003596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418755.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacyl-CoA dehydratase subunit D"
                     /protein_id="HCD1933874.1"
                     /translation="MSLVTDLPAIFDQFSEARQTGFLTVMDLKERGIPLVGTYCTFMP
                     QEIPMAAGAVVVSLCSTSDETIEEAEKDLPRNLCPLIKSSYGFGKTDKCPYFYFSDLV
                     VGETTCDGKKKMYEYMAEFKPVHVMQLPNSVKDDASRALWKAEMLRLQKTVEERFGHE
                     ISEDALRDAIALKNRERRALANFYHLGQLNPPALSGSDILKVVYGATFRFDKEALINE
                     LDAMTARVRQQWEEGQRLDPRPRILITGCPIGGAAEKVVRAIEENGGWVVGYENCTGA
                     KATEQCVAETGDVYDALADKYLAIGCSCVSPNDQRLQMLSQMVEEYQVDGVVDVILQA
                     CHTYAVESLAIKRHVRQQHNIPYIAIETDYSTSDVGQLSTRVAAFIEML"
     gene            4070..4837
                     /locus_tag="NBI74_003597"
     CDS             4070..4837
                     /locus_tag="NBI74_003597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001298028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1933875.1"
                     /translation="MTYSIGIDSGSTATKGILLADGVITRRFLVPTPFRPATAITEAW
                     ETLREGLETTPFLTLTGYGRQLVDFADKQVTEISCHGLGARFLEPATRAVIDIGGQDS
                     KVIQLDDDGNLCDFLMNDKCAAGTGRFLEVISRTLGTSVEQLDSITENVIPHAITSMC
                     TVFAESEVISLRSAGVAPEAILAGVINAMARRSANFIARLSCEAPILFTGGVSHCQRF
                     THMLESHLGMPVQTHPDAQFAGAIGAAVIGQRQRKRA"
     gene            4834..5091
                     /locus_tag="NBI74_003598"
     CDS             4834..5091
                     /locus_tag="NBI74_003598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3343 domain-containing protein"
                     /protein_id="HCD1933876.1"
                     /translation="MKEYLFLFHSTVGVIQTRKALQAAGMTFRVSDIPRDLRGGCGLC
                     IWLTCPPGEEIQWVIPGHTESVYCQQDGGWRCIAHYGISPR"
     gene            5156..6016
                     /locus_tag="NBI74_003599"
     CDS             5156..6016
                     /locus_tag="NBI74_003599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418753.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiK family protein"
                     /protein_id="HCD1933877.1"
                     /translation="MTKSISLSKRIFVIVILFVIVAVCTFFVQSCARKSNHAASFQNY
                     HATIDGKEIAGITNNISSLTWSAQSNTLFSTINKPAAIVEMTTNGDLIRTIPLDFVKD
                     LETIEYIGDNQFVISDERDYAIYVISLTPNSEVKILKKIKIPLQESPTNCGFEGLAYS
                     RQDHTFWFFKEKNPIEVYKVNGLLSSNELHISKDKALQRQFTLDDVSGAEFNQQKNTL
                     LVLSHESRALQEVTLAGEVIGEMSLTKGSRGLSHNIKQAEGVAMDASGNIYIVSEPNR
                     FYRFTPQSSH"
     gene            6084..7262
                     /locus_tag="NBI74_003600"
     CDS             6084..7262
                     /locus_tag="NBI74_003600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCD1933878.1"
                     /translation="MPSPSHPVERFSFSTALFGMLVLTLGMGLGRFLYTPMLPVMMAE
                     GSFSFSQLSWIASGNYAGYLAGSLLFSFGAFHQPSRLRPFLLASALASGLLILAMAWL
                     LPFILVLLIRVLAGVASAGMLIFGSTLIMQHTRHPFVLAALFSGVGIGIALGNEYVLA
                     GLHFDLSSQTLWQGAGALSGMMLIALTLLMPSKKHAITPMPLAKTEQQIMSWWLLAIL
                     YGLAGFGYIIVATYLPLMAKDAGSPLLTAHLWTLVGLSIVPGCFGWLWAAKRWGALPC
                     LTANLLVQAICVLLTLASDSPLLLIISSLGFGGTFMGTTSLVMTIARQLSVPGNLNLL
                     GFVTLIYGIGQILGPALTSMLGNGTSALAGATLCGAAALFIAALISTVQLFKLQVVTS
                     "
     gene            complement(7275..7829)
                     /gene="kptA"
                     /locus_tag="NBI74_003601"
     CDS             complement(7275..7829)
                     /gene="kptA"
                     /locus_tag="NBI74_003601"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2'-phosphotransferase"
                     /protein_id="HCD1933879.1"
                     /translation="MAKYNEKELADTSKFLSFVLRHKPEAIGIVLDREGWADIDKLIL
                     CAQKAGKRLTRALLDTVVATSDKKRFSYSSDGRCIRAVQGHSTSQVAISFAEKTPPQF
                     LYHGTASRFLDEIKKQGLIAGERHYVHLSADEATARKVGARHGSPVILTVKAQEMAKR
                     GLPFWQAENGVWLTSTVAVEFLEW"
     gene            8079..8762
                     /locus_tag="NBI74_003602"
     CDS             8079..8762
                     /locus_tag="NBI74_003602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1933880.1"
                     /translation="MTQQGDAVAGELATEKVGIKGYLAFFLTIIFFSGVFSGTDSWWR
                     VFDFSVLNGSFGQLPGANGATTSFRGAGGAGAKDGFLFALELAPSVILSLGIISITDG
                     LGGLRAAQQLMTPVLKPLLGIPGICSLALIANLQNTDAAAGMTKELAQEGEITERDKV
                     IFAAYQTSGSAIITNYFSSGVAVFAFLGTSVIVPLAVILVFKFVGANILRVWLNFEER
                     RNPTQGAQA"
     gene            8759..9220
                     /locus_tag="NBI74_003603"
     CDS             8759..9220
                     /locus_tag="NBI74_003603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiG family protein"
                     /protein_id="HCD1933881.1"
                     /translation="MTTQVRKNVMDMFIDGARRGFTIATTNLLPNVVMAFVIIQALKI
                     TGLLDWVGHICEPVMALWGLPGEAATVLLAALMSMGGAVGVAASLATAGALTGHDVTV
                     LLPAMYLMGNPVQNVGRCLGTAEVNAKYYPHIITVCVINALLSIWVMQLIV"
     gene            9233..10405
                     /gene="iadA"
                     /locus_tag="NBI74_003604"
     CDS             9233..10405
                     /gene="iadA"
                     /locus_tag="NBI74_003604"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HCD1933882.1"
                     /translation="MIDYTAAGFTLLQGAHLYAPEDRGICDVLVANGKIIAVASNIPS
                     DIVPDCTVVDLSGQILCPGFIDQHVHLIGGGGEAGPTTRTPEVALSRLTEAGVTSVVG
                     LLGTDSISRHPESLLAKTRALNEEGISAWMLTGAYHVPSRTITGSVEKDVAIIDRVIG
                     VKCAISDHRSAAPDVYHLANMAAESRVGGLLGGKPGVTVFHMGDSKKALQPVYDLLEN
                     CDVPISKLLPTHVNRNVPLFEQALEFARKGGTIDITSSIDEPVAPAEGIARAVQAGIP
                     LARVTLSSDGNGSQPFFDDEGNLTHIGVAGFETLLETVQVLVKDYDFSISDALRPLTS
                     SVAGFLNLSGKGEILPGNDADLLVMTPELRIEQVYARGKLMVKDGKACVKGTFETA"
     gene            10470..11381
                     /gene="hypT"
                     /locus_tag="NBI74_003605"
                     /gene_synonym="qseD"
     CDS             10470..11381
                     /gene="hypT"
                     /locus_tag="NBI74_003605"
                     /gene_synonym="qseD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypochlorite stress DNA-binding transcriptional
                     regulator HypT"
                     /protein_id="HCD1933883.1"
                     /translation="MDDCGAVLHNIETKWLYDFLTLEKCRNFSQAAVSRNVSQPAFSR
                     RIRALEQAIGVELFNRQVTPLQLSEQGKIFHSQIRHLLQQLESNLAELRGGSDYAQRK
                     IKIAAAHSLSLGLLPSIISQMPPLFTWAIEAIDVDEAVDKLREGQSDCIFSFHDEDLL
                     EAPFDHIRLFESQLFPVCASDEHGEALFNLAQPHFPLLNYSRNSYMGRLINRTLTRHS
                     ELSFSTFFVSSMSELLKQVALDGCGIAWLPEYAIQQEIRSGQLVVLNRDELVIPIQAY
                     AYRMNTRMNPVAERFWRELRELEIVLS"
     gene            complement(11374..11766)
                     /gene="iraD"
                     /locus_tag="NBI74_003606"
     CDS             complement(11374..11766)
                     /gene="iraD"
                     /locus_tag="NBI74_003606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418746.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraD"
                     /protein_id="HCD1933884.1"
                     /translation="MMRQSLQAVLPEISGNKTSPLRKSVCSDLLTLFNSPHSALPSLL
                     VSGMPEWQVHNPSDKHLQSWYCRQLRSALLFHEPRIAALQVNLKEAYCHTLAISLEIM
                     LYHDDESLTFDLVWDNGGWRSATLENVS"
     gene            complement(11763..11846)
                     /gene="idlP"
                     /locus_tag="NBI74_003607"
     CDS             complement(11763..11846)
                     /gene="idlP"
                     /locus_tag="NBI74_003607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iraD leader peptide IdlP"
                     /protein_id="HCD1933885.1"
                     /translation="MENEHQYSGARCSGQAAYVAKRQECAK"
BASE COUNT         2748 a         3062 c         3152 g         3144 t
ORIGIN      
        1 attccataat gattccacgc cccgttatga caaatctgcc gccgatcttg cctggcaaag
       61 gacactgaaa tggtttgata aatatctctc ctgataggtt tatctcttac gggattacgt
      121 cttaaacaag catgaaaaaa tagcgtgcgc aaaagtcgtt ctttgcctaa aatatcgcta
      181 tatatggcga tatatagcaa ataaagtgaa cgatgaataa ccattttggt aaaggcttaa
      241 tggcgggatt aaaagcaacg catgccgcca cgctgttttt cccgatggcg ttgattttgt
      301 atgattttgc cgcgtacctg tcgacggacc tgattcagcc ggggatcatt aatgtggtcc
      361 gtgattttaa tgccgatgtc agtctggccc cggcagccgt cagtctctat ctcgcgggcg
      421 gtatggcgtt acagtggctg ctggggccgc tttccgacag gattggccgc aggccggtgc
      481 tgattaccgg ggcgctaata tttacccttg cctgcgctgc gacaatgttc acaacgtcta
      541 tgacacagtt tcttatcgcg cgtgcaattc agggcaccag tatctgtttt attgccaccg
      601 ttggttatgt cacggtgcag gaggcgttcg gccagacaaa agggatcagg ttgatggcga
      661 ttatcacctc catcgtactg attgcgccga ttatcggccc gctttccggc gcagctctga
      721 tgcactttgt acactggaaa gtcctttttg ccatcattgc gattatgggt tttatctcat
      781 ttgttggctt actgctggcg atgccagaga cggtgaagcg cggcgcggtt tcatttagcg
      841 ccaaaagcgt cttgcgcgat tttcgtaatg tcttttgcaa tcggcagttc ctctttggtg
      901 cagcaaccat ctctttaagc tatatcccaa tgatgagttg ggtggctgtc tcgccggtga
      961 tcctgattga cgcaggcggc ttaacgacct cgcagttcgc ctggacacag gttccggtgt
     1021 tcggcgcggt gattgtcgcg aatgccatcg tggcgcgttt cgttaaagat ccgaccgaac
     1081 cgcgctttat ctggcgtgcc gtacccattc aactggttgg cctcgcgctg ttgattatcg
     1141 gcaatctgct gtcaccgcac gtatggctgt ggtcggtgct gggcaccagt ttgtatgctt
     1201 tcgggattgg tttgatcttc ccgaccttat tccgctttac gctgttttcc aataacttac
     1261 cgaaagggac cgtctccgca tcgctgaata tggtgatcct gatggtgatg tctgtctcgg
     1321 tcgaaatcgg ccggtggctg tggtttaacg gcggtcgctt gccgtttcat ctgttagccg
     1381 tcgtggcggg cgttatcgtc gttttcaccc tggcgggatt gcttaatcgc gtgcgccaac
     1441 atcaggctgc cgagctggca gaggagcagt gatttttgcg cgatcctgcc gtcaggctct
     1501 attcttaacg ttatgaataa actcattgaa ctcagacgcg ccaaaatgtt ggcgctctct
     1561 ttactgctta tcgccgctgc tacctttgtc gttacgctgt ttttgccgcc caatttttgg
     1621 gtgagcggcg tgaaggcgat tgctgaagcg gcgatggtcg gcgcgctggc ggactggttt
     1681 gcggtggtgg cgctgtttcg ccgcgtgccg attccgatca tttctcgcca tacggcgatt
     1741 atcccgcgta ataaagaccg gattggcgaa aatctcggcc agttcgtgca ggaaaaattt
     1801 cttgatactc aatccctggt ggcattgatt cgacgccacg aaccggcgtt gctgattggc
     1861 aactggttta gccagccaga aaacgcccgc cgcgttggtc agcatctgtt gcagatcatg
     1921 agcggttttc tcgaacttac cgatgatgcg cgtattcagc gcctgcttaa gcgcgcggtc
     1981 catcgggcga ttgataaggt cgatctttcc ggcaccagtg cgttgatgct ggagagtatg
     2041 accaaaaacg atcgtcatca ggtgctgctg gatacgctga tcgcacagtt gatcgccctt
     2101 ctccagcgcg ataaatcgcg caagtttatc gcccagcaaa ttgttcgctg gctggagagc
     2161 gagcatccac tgaaagccaa aattctcccc accgaatggt tgggcgaaca tagcgcggag
     2221 ttggtttctg acgcggtgaa ttctttgctt gatgatatta gtcgcgatcg tgcgcatcag
     2281 atccgccatg cgtttgatcg cgccaccttc gccctgatcg acaagctgaa aaacgatccg
     2341 gaaatggcag cgcgagccga tgccgtaaaa agctatctga aagaagatga agattttaat
     2401 cgctatctca gtgaattgtg gggggattta cgggaatggc tgaaagtgga tatcaacagt
     2461 gaagattctc gtgtgaaaga acgcatcgca cgagcgggtc aatggtttgg cgaaacgtta
     2521 attgccgatg atgccttgcg ggcgtcgtta aatggtcatc ttgaacaagc cgcgcaccgc
     2581 gtcgcgcctg agttttccgc attcctgacg cgccacatca gcgatacggt aaaaagctgg
     2641 gatgcgcggg atatgtcgcg gcaaatagag ttaaatatcg gcaaagatct gcagtttatt
     2701 cgtgtcaacg gtacgctggt tggcggttgt attgggctaa ttttatattt gctgtcgcag
     2761 ctcccggcct tgttccccct cggcaatttt tagaaatcca tgaaaaacta agtggctaac
     2821 cgcgagagag atcaaataaa ccatactaaa agaatggtaa taatgtggcc tcttttcatt
     2881 atctcccgtg gtacggggaa ggaaaatcat gtcacttgtc accgatctac ccgccatttt
     2941 cgatcagttc tctgaagctc gccagacagg ctttctcacc gtcatggatc tcaaggagcg
     3001 cggcattccg ctggttggca cttactgcac ctttatgccg caagagatcc cgatggcagc
     3061 cggtgcggtt gtggtttcgc tctgttccac ctctgatgaa accattgaag aagcggagaa
     3121 agatctgccg cgcaacctct gcccgctgat taaaagcagc tacggcttcg gcaaaaccga
     3181 taaatgcccc tacttctact tttcggatct ggtggtcggt gaaaccacct gcgacggcaa
     3241 aaagaaaatg tatgaataca tggcggagtt taagcctgtt catgtgatgc aattgcccaa
     3301 cagcgttaag gacgatgcct cgcgtgcgtt atggaaagcc gagatgctgc gcttgcaaaa
     3361 aacggtagaa gaacgttttg ggcacgagat tagcgaagat gctctgcgcg atgccattgc
     3421 gctgaaaaac cgcgaacgtc gcgcactggc taatttttat catcttgggc agttaaatcc
     3481 tccggcgctt agcggcagcg acattctgaa agtggtttac ggcgcaacct tccggttcga
     3541 taaagaggcg ttgatcaatg aactggatgc aatgaccgcc cgcgttcgtc agcagtggga
     3601 agaaggccag cgactggacc cgcgtccgcg cattttaatc accggctgcc cgattggcgg
     3661 cgcagcagaa aaagtggtgc gcgcgattga agagaatggc ggctgggttg tcggttatga
     3721 aaactgcacc ggggcgaaag cgaccgagca atgcgtggca gaaacgggcg atgtctacga
     3781 cgcgctggcg gataaatatc tggcgattgg ctgctcctgt gtttcgccga acgatcagcg
     3841 cctgcaaatg ctcagccaga tggttgaaga atatcaggtc gatggcgtag ttgatgtgat
     3901 tttgcaggcg tgccatacct acgcggtgga atcgctggcg attaaacgtc atgtgcgcca
     3961 gcagcacaac attccttata tcgctattga aacagactac tccacctcgg atgtcgggca
     4021 gctcagcacc cgtgtcgcgg cctttattga gatgctgtaa ggagtggcag tgacatattc
     4081 gattggcatt gattccggct caaccgccac caaagggatc ttactggctg acggcgtgat
     4141 tacgcgccgt ttcctcgttc caacaccctt tcgcccggca acagcaatta ctgaagcctg
     4201 ggaaactctg cgcgaagggt tagagacaac gccgtttctg acgctcaccg gctacggacg
     4261 gcaactggtg gattttgccg ataaacaagt gacggaaatc tcctgtcacg ggctgggcgc
     4321 acggtttctt gagccagcaa cgcgcgcggt aatcgacatc ggtggtcagg acagcaaagt
     4381 gattcagctt gatgatgacg gtaacctgtg cgatttcctg atgaatgaca aatgcgcggc
     4441 gggcaccggg cgtttcctgg aggtgatctc gcgcacgctt ggcaccagcg tcgagcaact
     4501 cgacagcatt accgaaaatg tcataccgca cgccatcacg agtatgtgca cagtgtttgc
     4561 tgaatcagaa gtgatcagcc tgcgctctgc gggcgtagcg ccagaagcga ttctcgccgg
     4621 agtgattaac gcgatggcgc ggcggagcgc caatttcatt gctcgtctct cctgtgaagc
     4681 gccgattctg tttactggtg gcgttagcca ctgtcaaagg tttacccaca tgctggaaag
     4741 ccatctggga atgccggtac aaactcaccc tgatgcgcaa ttcgctggtg caattggcgc
     4801 ggcggtgatc ggtcagcggc agagaaaacg cgcatgaaag agtatctgtt tttatttcac
     4861 tccacggtcg gcgtcataca aacccgcaag gcgttgcagg cagcgggcat gacttttcgc
     4921 gtcagcgata ttccccgtga tttacgcggc ggctgcgggt tatgcatttg gctgacctgt
     4981 ccgcctggcg aggaaattca atgggtgatc cctgggcata cggaatcagt ttattgtcag
     5041 caggatggtg gctggcgctg cattgcacat tacgggattt ctccgcgtta aggattcatt
     5101 aagatcgcta cgtatacttc tcgatatctt attgatttgc cttaaaggga ttaccgtgac
     5161 aaaaagtatt tcgttaagta aacgcatctt cgttattgtg attctttttg tcattgtggc
     5221 agtgtgtact ttcttcgtgc aatcttgcgc ccgtaaaagc aatcatgcag catcatttca
     5281 gaattaccac gccaccattg acggcaaaga aattgcaggc ataacgaata atatctcttc
     5341 gctgacctgg tcggcacaaa gcaatactct ttttagcact atcaacaaac ctgccgccat
     5401 tgttgaaatg accaccaatg gcgatttgat ccgcacaatt cctctggatt ttgttaagga
     5461 tctcgagacg atcgaataca ttggcgataa tcagtttgtc attagcgatg agcgcgatta
     5521 tgccatttat gtgatttcac tgaccccaaa ctcggaagta aaaatcctca aaaaaataaa
     5581 aatcccgctg caggaatccc caaccaattg cggttttgaa ggattggcct attctcgcca
     5641 ggatcacact ttctggtttt ttaaagagaa aaacccgatt gaggtctaca aagttaacgg
     5701 gctgttaagt agcaatgaat tgcacatcag caaagacaag gcactacaac ggcaatttac
     5761 tttagatgat gtgtccggcg cagaatttaa ccaacaaaaa aatacgcttc tggtgctgtc
     5821 tcatgaatct cgcgcactcc aggaagtaac gctggctggc gaggttatag gtgaaatgtc
     5881 actaaccaaa ggcagtcgag gtttgtcgca taacatcaaa caggccgaag gggttgcgat
     5941 ggatgcctca ggtaatattt atatcgttag cgaaccaaat cgtttttatc gctttacccc
     6001 tcaatcatcg cattaacagg ctggcatgtt tcaccttttt gttattaact tgttaacaac
     6061 aaaaatgaca aacaaggtta aacatgcctt cgccctcgca tcccgtagaa cgcttttctt
     6121 tcagcaccgc gcttttcggg atgctggttc tgaccttagg tatgggttta ggccgcttcc
     6181 tttatacgcc tatgttgccc gtcatgatgg cggaaggatc gttttcattt agccagctct
     6241 cgtggattgc cagcggcaac tatgcggggt atctggctgg cagtctgcta ttttcgtttg
     6301 gcgcatttca ccagccatcg cgcctgcgcc catttctgtt ggcttctgcc ctggcgagcg
     6361 ggttgttgat cctcgcaatg gcatggttgc tgccatttat tctggtgtta ttgattcgcg
     6421 tcctggcggg tgtcgccagc gccggtatgc tgatttttgg ttcgacgctg attatgcagc
     6481 acacgcgcca tccttttgtg ctggcagctt tgttttctgg cgttggcatt ggcatcgcac
     6541 tgggcaatga atatgttctg gcaggcctgc attttgacct ctcctcgcaa acgttatggc
     6601 aaggcgccgg ggcgctttct ggcatgatgc tgattgcact tacgctttta atgccctcga
     6661 aaaaacacgc catcacacca atgccattgg caaaaacgga gcaacagata atgagctggt
     6721 ggttactggc tattctgtac ggcctggcgg gatttggtta tatcatcgtc gcgacttacc
     6781 tgccgctcat ggcgaaagat gcaggctcac cattgttaac cgcccatctc tggacgttag
     6841 tcggcttatc aattgtgcct ggttgctttg gctggctatg ggcagcaaaa cgttgggggg
     6901 ctttgccctg cctgacggcg aatttgctgg tgcaggctat ctgtgtgctg cttactctcg
     6961 ccagcgactc gcctctcttg cttatcatca gcagtcttgg ttttggcggt acctttatgg
     7021 gcacgacatc tctggtgatg actatcgccc gccaactgag cgtaccggga aatctcaatc
     7081 ttttaggctt tgtgacgctc atttacggca ttggacaaat cctcggcccg gcgctgacca
     7141 gtatgctcgg caatggaaca tcggcgcttg ccggcgccac tctctgcggc gcggcggcgt
     7201 tgtttattgc tgcgcttatc agcacagtac aattatttaa gctgcaagtg gtcacttcat
     7261 gaggagtgtt tcccttacca ctcaagaaat tcgaccgcaa ccgttgatgt cagccagacc
     7321 ccgttttccg cttgccagaa agggaggcct cgtttcgcca tttcctgtgc tttgacggtt
     7381 aaaataaccg gcgatccgtg tcttgctccc actttgcgtg ccgttgcttc atcagcagag
     7441 agatgtacgt agtgacgctc acctgcaatc agcccctgtt tttttatctc gtcaaggaaa
     7501 cggcttgccg taccgtggta gagaaactgt ggcggtgttt tttcggcaaa ggaaatagcg
     7561 acctgcgaag tcgaatgccc ctgaaccgcg cggatgcacc taccatcact ggaataacta
     7621 aaacgctttt tatcgctggt ggcaaccacg gtatccagca atgcacgggt taaccgcttg
     7681 cccgcttttt gggcacaaag tatgagctta tcgatatccg cccatccttc acggtccagt
     7741 acgataccaa tcgcctccgg tttgtgtcgg aggacaaaac ttaaaaactt gctcgtatcc
     7801 gccagctctt tttcgttgta ctttgccatc gcgttttcgt ccctggatta tctgtttttt
     7861 atttactatg tcattgtcac tgggtttatg aattttctgc aactcaatcc tgcgtagaac
     7921 cttttccaca cagcgtaatt ttaccgctat gcaaaaaacg catagtcaga aacatacttg
     7981 cattttcccc atacccacat ggctgataag gtttttcagc tccaggaatt ttcctggctc
     8041 aagcaacaca caaacacaaa aaaacaatgg ggattgttat gacacaacaa ggggatgctg
     8101 tagccgggga gctggcgaca gaaaaagtgg gcattaaagg gtatctggct ttttttctca
     8161 ccataatctt tttttccggt gttttttccg ggacagacag ttggtggcgc gtttttgatt
     8221 tttccgttct gaacggatcg tttgggcaac tgcctggtgc gaacggagcc accacctcgt
     8281 ttcgcggcgc gggtggcgcg ggggctaaag atggctttct ctttgcgctg gagctggcac
     8341 cgtcagtcat tctctctctg gggattattt caattaccga tggtcttggc ggattacgcg
     8401 ccgcgcagca gttaatgacg ccagtgctaa aaccgttgtt gggcattcca gggatctgct
     8461 cgctggcgct gatcgctaac ctgcaaaaca ccgatgccgc cgcaggtatg accaaagagc
     8521 tggcgcagga aggtgaaatt accgaacgcg ataaagtgat tttcgccgca tatcaaacca
     8581 gcggtagcgc cattatcacc aactacttct cttccggcgt cgcggtgttt gcctttctgg
     8641 gcacgtccgt aatcgtacct ttagcagtca tcctggtgtt taaatttgtc ggagccaaca
     8701 ttttgcgcgt ctggcttaac ttcgaagaac gtcgcaaccc aacgcaagga gcgcaagcat
     8761 gacaactcag gtccgcaaaa acgtcatgga tatgtttatc gatggtgcgc gccgtggctt
     8821 tactatcgcc acgaccaacc tgctgccaaa cgtggtgatg gcgtttgtca tcattcaggc
     8881 gctgaaaatt accggcctgc tcgactgggt ggggcatatc tgtgaacccg taatggcgct
     8941 gtggggatta cccggtgaag cggcaaccgt ccttttagcc gcattaatga gtatgggtgg
     9001 cgcagtcggt gttgccgcca gtctggcaac cgctggcgca ttaaccggtc acgatgtcac
     9061 cgttttactg cccgcgatgt atttaatggg caacccggtg caaaatgtcg gtcgctgcct
     9121 ggggacagcc gaagtgaatg ccaaatatta cccgcatatc attacggtct gcgtgattaa
     9181 cgcattgctg tcgatctggg ttatgcagct tattgtttaa caaggagtta ccatgattga
     9241 ttataccgca gccggtttta ccctgctgca gggagcgcat ttgtatgcgc cggaagatcg
     9301 gggaatttgc gatgtcctcg tcgctaacgg caaaattatc gccgttgcca gtaatatccc
     9361 ttctgacatt gtaccggact gcacggttgt cgatctcagc gggcagatcc tctgcccagg
     9421 ttttattgat caacacgtcc atttgattgg cggtggcggc gaagcaggtc ccacgacgcg
     9481 cacgccggaa gtggcgttaa gtcgtctgac ggaagcaggc gtcacgtcag tggttggtct
     9541 gctgggcacc gactctatct ctcgccaccc ggaatccctg ctcgccaaga cccgtgcgct
     9601 caatgaagaa ggcatcagcg cctggatgct gaccggcgct tatcatgtcc cttcccgcac
     9661 tattacgggt tccgtggaaa aagacgtggc gattatcgat cgcgtgattg gcgtgaaatg
     9721 cgccatctct gatcaccgtt ctgccgcacc ggacgtttat cacctggcta atatggcggc
     9781 ggaatcccgc gttggcggtt tgctcggcgg taaacctggc gtcaccgtgt tccacatggg
     9841 cgacagcaaa aaggcgttac agcctgtcta tgacctgctg gaaaactgcg atgtgccgat
     9901 tagcaagttg ctgccgaccc acgttaaccg caacgttccg ttgtttgagc aggcgctgga
     9961 gttcgcgcgc aaaggcggca ccatcgatat caccagcagc attgacgaac cagtcgcccc
    10021 tgccgaaggt attgcccgcg ccgttcaggc gggtattccg ctggcacgcg tcaccctcag
    10081 ctccgacggc aacggtagcc agccgttctt cgatgacgaa gggaatttaa cccatatcgg
    10141 tgttgccggt tttgaaacgt tgctggaaac cgtgcaggtg ctggtcaaag attatgattt
    10201 cagtatcagc gatgccctgc gcccgctcac cagtagcgta gccggtttcc ttaacctgag
    10261 cgggaaaggc gaaattctgc caggcaatga cgcagactta ctggtcatga cgccagaatt
    10321 acgcattgag caggtatacg ctcgcggcaa gctgatggtc aaagacggca aagcctgcgt
    10381 gaaaggaacg tttgaaacgg cataaacggt tgtcagccgc ccgaatggac gtagaatgcc
    10441 cgcaagggcg gctgacagag taaaacgtaa tggatgactg tggtgcggtt ttgcataata
    10501 ttgaaactaa atggctttat gattttctga ccctggaaaa atgccgcaat ttttcccagg
    10561 cagcagtcag tcgcaacgtc tcgcaaccgg cattcagccg ccgcatccgt gcgctggaac
    10621 aggcgattgg cgttgaattg tttaaccgcc aggtgacgcc gctgcaactc tcggaacaag
    10681 gtaaaatatt ccattcgcag atccgccatc tgttgcaaca gttagagagc aaccttgcag
    10741 aactccgtgg tggcagcgat tacgcgcaac gcaaaattaa aatagccgct gcacactctc
    10801 tttccctcgg gctgttaccg tccattatca gccagatgcc gccgctcttt acctgggcaa
    10861 ttgaagctat tgatgtcgat gaagcggtcg ataaactgcg tgaagggcaa agtgactgta
    10921 ttttttcctt tcacgacgaa gatttgctgg aagcgccgtt tgaccacatt cgcttatttg
    10981 aatctcaatt gttccccgtt tgcgccagtg acgaacacgg agaagcactt tttaacctcg
    11041 cgcagccaca ctttccgtta ctgaattaca gccgcaactc ctacatgggg cgattgatta
    11101 atcgcaccct gacgcgccac agtgagttaa gtttcagcac cttttttgtc tcttcgatga
    11161 gcgagctttt aaagcaggtt gccctcgacg gctgtgggat tgcctggcta ccggaatacg
    11221 ccatccaaca agaaattcgc agcgggcagc tcgttgtact gaatcgggac gaactggtga
    11281 tcccgattca ggcctatgca taccggatga atacccgcat gaatccagtt gccgaacgct
    11341 tctggcgtga actgcgcgag ctggagattg tgcttagctg acattctcca gcgtcgcact
    11401 gcgccaaccg ccattatccc agaccagatc aaacgttagc gattcatcgt cgtgatacag
    11461 cattatctca aggctgatcg ccagagtgtg gcagtacgct tctttcaaat taacctgcaa
    11521 cgccgcaatc cgtggctcat gaaataacag tgcagaacgc agctgacggc aataccagct
    11581 ctggagatgt ttgtcgctcg ggttatgcac ttgccactcg ggcatgcctg aaacaagtaa
    11641 tgacggtaat gccgaatgag gagaattaaa aagagtgagg agatcgctgc atactgattt
    11701 acgcagcggt gacgttttat tgcctgaaat ttcaggtaaa acagcctgaa gtgattggcg
    11761 catcattttg cgcactcctg acgtttagca acatatgcgg cttgccctga acaccgggca
    11821 ccactgtatt gatgctcatt ttccatagcc attccctttt gttcattaca tccatgtcag
    11881 tctgagacag gcctgaatcc tgtcagcaaa gtataaaacc ttacttgcct atgaatatct
    11941 actgactttt caggaaaaaa gtcatgattt gataaaaatc atcaaactca aaaaaaaacc
    12001 gctagtatat tctgaatatt ccgaatagtt acgtagatga aaatacttgt caccaccgct
    12061 acttaattcc ggtgggaaat tatttatttc cgcataatca gccatg
//
LOCUS       DAHHTG010000123        12073 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.124, whole genome shotgun sequence.
ACCESSION   DAHHTG010000123 DAHHTG010000000
VERSION     DAHHTG010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12073)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12073)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12073
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.124"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            145..1533
                     /gene="narU"
                     /locus_tag="NBI74_003608"
     CDS             145..1533
                     /gene="narU"
                     /locus_tag="NBI74_003608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite transporter NarU"
                     /protein_id="HCD1933886.1"
                     /translation="MALQNEKNSRYLLRDWKPENPAFWENKGKHIARRNLWISVSCLL
                     LAFCVWMLFSAVTVNLNKIGFNFTTDQLFLLTALPSVSGALLRVPYSFMVPIFGGRRW
                     TVFSTAILIIPCVWLGIAVQNPNTPFGIFIVIALLCGFAGANFASSMGNISFFFPKAK
                     QGSALGINGGLGNLGVSVMQLVAPLVIFVPVFAFLGVNGVPQADGSVMSLANAAWIWV
                     PLLAIATIAAWSGMNDIASSRASIADQLPVLQRLHLWLLSLLYLATFGSFIGFSAGFA
                     MLAKTQFPDVNILRLAFFGPFIGAIARSVGGAISDKFGGVRVTLINFIFMAIFSALLF
                     LTLPGTGSGNFIAFYAVFMGLFLTAGLGSGSTFQMIAVIFRQITIYRVKMKGGSDEQA
                     QREAVTETAAALGFISAIGAVGGFFIPQAFGMSLNMTGSPVGAMKVFLIFYIVCVLLT
                     WLVYGRRKFSQK"
     gene            1628..5368
                     /gene="narZ"
                     /locus_tag="NBI74_003609"
     CDS             1628..5368
                     /gene="narZ"
                     /locus_tag="NBI74_003609"
                     /EC_number="1.7.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase Z subunit alpha"
                     /protein_id="HCD1933887.1"
                     /translation="MSKLLDRFRYFKQKGEIFADGHGQVMHSNRDWEDSYRQRWQFDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWEIQQTDYPRTRPDLPNHEPRGCPRGASYSWY
                     LYSANRLKYPLIRKRLIELWREALKQHSDPVLAWASIMNDPQKCLSYKQVRGRGGFIR
                     SNWQELNQLIAAANVWTIKTYGPDRVAGFSPIPAMSMVSYAAGTRYLSLLGGTCLSFY
                     DWYCDLPPASPMTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTIAITPDYSEVAKLCDQWLAPKQGTDSALAMAMGHVILKEFHLDNPSDYFINYCRRY
                     SDMPMLVMLEPRDDGSYVPGRMVRASDLVDGLGESNNPQWKTVAVNTAGELVVPNGSI
                     GFRWGEKGKWNLESIAAGTETELSLTLLGQHDAVAGVAFPYFGGIENPHFRSVKHNPV
                     LVRQLPVKNLTLADGSTCPVVSVYDLVLANYGLDRGLEDENSAKDYAEIKPYTPAWGE
                     QITGVPRQYIETIAREFADTAHKTHGRSMIILGAGVNHWYHMDMNYRGMINMLIFCGC
                     VGQSGGGWAHYVGQEKLRPQTGWLPLAFAFDWNRPPRQMNSTSFFYNHSSQWRYEKVT
                     AQELLSPLADASKYSGHLIDFNVRAERMGWLPSAPQLGRNPLSLKAEADKAGLSPTEF
                     TAQALKSGDLRMACEQPDSGSNHPRNLFVWRSNLLGSSGKGHEYMQKYLLGTESGIQG
                     EELGASDGIKPEEVEWQTAAIEGKLDLLVTLDFRMSSTCLFSDIVLPTATWYEKDDMN
                     TSDMHPFIHPLSAAVDPAWESRSDWEIYKGIAKAFSQVCVGHLGKETDVVLQPLLHDS
                     PAELSQPCEVLDWRKGECDLIPGKTAPNIVAVERDYPATYERFTSLGPLMDKLGNGGK
                     GISWNTQDEIDFLGKLNYTKRDGPAQGRPLIDTAIDASEVILALAPETNGHVAVKAWQ
                     ALGEITGREHTHLALHKEDEKIRFRDIQAQPRKIISSPTWSGLESDHVSYNAGYTNVH
                     ELIPWRTLSGRQQLYQDHPWMRAFGESLVAYRPPIDTRSVSEMRQIPPNGFPEKALNF
                     LTPHQKWGIHSTYSENLLMLTLSRGGPIVWISETDARELTIVDNDWVEVFNANGALTA
                     RAVVSQRVPPGMTMMYHAQERIMNIPGSEVTGMRGGIHNSVTRVCPKPTHMIGGYAQL
                     AWGFNYYGTVGSNRDEFIMIRKMKNVNWLDDEGRDQVQEAKK"
     gene            5365..6909
                     /gene="narH"
                     /locus_tag="NBI74_003610"
     CDS             5365..6909
                     /gene="narH"
                     /locus_tag="NBI74_003610"
                     /EC_number="1.7.99.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HCD1933888.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTGREGMEYAWFNNV
                     ETKPGIGYPKNWEDQDEWQGGWVRDVNGKIRPRLGSKMGVITKIFANPVVPQIDDYYE
                     PFTFDYEHLHSAPEGKHIPTARPRSLIDGKRMDKVIWGPNWEELLGGEFEKRARDRNF
                     EAMQKEMYGQFENTFMMYLPRLCEHCLNPSCVATCPSGAIYKREEDGIVLIDQDKCRG
                     WRLCISGCPYKKIYFNWKSGKSEKCIFCYPRIESGQPTVCSETCVGRIRYLGVLLYDA
                     DRIEEAASTEREVDLYERQCEVFLDPHDPSVIEEALKQGIPQNVIDAAQRSPVYKMAM
                     DWKLALPLHPEYRTLPMVWYVPPLSPIQSYADAGGLPKSEGVLPAIESLRIPVQYLAN
                     MLSAGDTGPVLRALKRMMAMRHYMRSQTVEGVTDTRAIDEVGLSVAQVEEMYRYLAIA
                     NYEDRFVIPTSHREMAGDAFAERNGCGFTFGDGCHGSDSKFNLFNSSRIDAINITEVR
                     DKAEGE"
     gene            6909..7604
                     /gene="narW"
                     /locus_tag="NBI74_003611"
     CDS             6909..7604
                     /gene="narW"
                     /locus_tag="NBI74_003611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HCD1933889.1"
                     /translation="MQILKVLGLLMEYPDELLWECKDDALALIRRDAPMLTDFALDLL
                     NAPLLDKQAEWCEVFDRGRTTSLLLFEHVHAESRDRGQAMVDLLAEYEKVGLQLNCRE
                     LPDYLPLYLEYLSVLPDDQAKEGLLNVAPILTLLGGRLKQREAPWYALFDALLQLAGS
                     PLSSDSVTKQIRSEERDDTRQALDAVWEEEQVKFIEDNATACDSSPLNQYQRRFSQDV
                     APQYVDISAGGGK"
     gene            7601..8281
                     /gene="narI"
                     /locus_tag="NBI74_003612"
     CDS             7601..8281
                     /gene="narI"
                     /locus_tag="NBI74_003612"
                     /EC_number="1.7.99.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HCD1933890.1"
                     /translation="MIQYLNVFFYDIYPYLCGTVFILGSWLRYDYGQYTWRAASSQML
                     DKRGMVLWSNLFHIGILGIFFGHLFGMLTPHWMYAWFLPVAVKQQMAMILGGICGVLT
                     LIGGAGLLVRRLTNPRIRATSSGADILILAILLIQCILGLTTIPFSAQHPDGSEMMKL
                     VGWAQSIVTFQGGASTHLDGVAFIFRVHLVLGMTIFLLFPFTRLVHVWSAPFEYFTRR
                     YQIVRSRR"
     gene            8361..9254
                     /locus_tag="NBI74_003613"
     CDS             8361..9254
                     /locus_tag="NBI74_003613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family isomerase"
                     /protein_id="HCD1933891.1"
                     /translation="MKPQVYHVDAFTSQPFRGNSAGVVFPADNLSEAQMQLIARELGH
                     SETAFLLHSDDSDVRIRYFTPTVEVPICGHATVAAHYVRSKVLGLGNCTVWQTSLAGK
                     HRVTIEKQNDDYRISLEQGTPGFEPPLTGETRAAIINALHLTEDDILPGLPIQVATTG
                     HSKVMIPLKPEVDIDALSPDLAALTAISKQIGCNGFFPFQIRPGKSETDGRMFSPAIG
                     IVEDPVTGNANGPMGAWLVHHNVLPHDGNVLRVKGHQGRALGRDGVIDVTVTIRDNQP
                     EKVTISGTAVILFHAEWAIDF"
     gene            complement(9350..10195)
                     /gene="nhoA"
                     /locus_tag="NBI74_003614"
     CDS             complement(9350..10195)
                     /gene="nhoA"
                     /locus_tag="NBI74_003614"
                     /EC_number="2.3.1.118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-hydroxyarylamine O-acetyltransferase"
                     /protein_id="HCD1933892.1"
                     /translation="MTPILNHYFARINWLGAAAANIDTLRALHLKHNCTIPFENLDVL
                     LPREIQLDDQSLEEKLVIARRGGYCFEQNGVFERVLRELGFNVRSLLGRVVLSNPPAL
                     PPRTHRLLLVELEGEKWIADVGFGGQTLTAPIRLMPDIMQTTPHGEYRLLQEGDDWVL
                     QFNHHQHWQSMYRFDLCEQQQSDYVIGNFWSAHWPQSHFRHHLLMCRHLPDGGKLTLT
                     NFHFTHYENGHAVEQRNLPDVASLYAVMQEQFGLGVDDAKHGFTVDELALVMAAFDTH
                     PEAGK"
     gene            10370..10939
                     /locus_tag="NBI74_003615"
     CDS             10370..10939
                     /locus_tag="NBI74_003615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415979.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HCD1933893.1"
                     /translation="MSQFIPVELHHASCLLNHGPTVMITSFDEQSQRRNIMAAAWSMP
                     VEFEPPRVAIVVDKSTWTRELIEGNGKFGIVIPGVAATNWTWAVGSVSGREEDKFNCY
                     GIPVVRGPVLGLPLVEEKCLAWMECRLLPATSAQEKYDTLFGEVVSAAADARVFVEGR
                     WQFDDDKLNTLHHLGAGMFVTSGKRVTAG"
     gene            complement(11010..>12073)
                     /locus_tag="NBI74_003616"
     CDS             complement(11010..>12073)
                     /locus_tag="NBI74_003616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ISAs1 family transposase"
                     /protein_id="HCD1933894.1"
                     /translation="LSDILLLTICAVISGAEGWEDIEDFGETHLDFLKQYGDFDNGIP
                     VHDTIARVVSCISPAKFHECFINWMRDCHSSDDKDVIAIDGKTLRHSYDKSRRKGAIH
                     VISAFSTMHSLVIGQIRTDEKSNEITPIPELLNMLDIKGKIITTDAMGCQKDIAEKIQ
                     KQGGDYLFAVKGNQGRLNKAFEEKFPLKELNNPEHDSYAISEKSHGREEIRLHIVCDV
                     PDELIDFTFEWKGLKKLCVAVSFRSIIAEQKKEPEMTVRYYISSADLTAEKFATAIRN
                     HWHVENKLHWRLDVVMNEDDCKIRRGNAAELFSGIRHIAINILTNDKVFKAGLRRKMR
                     KAAMDRNYLASVLAGSGLS"
BASE COUNT         2834 a         3147 c         3115 g         2977 t
ORIGIN      
        1 ttctgccccc gcagttacgc tttcttatct attcttctaa tcagcacttc acctgattca
       61 tccttaattc taataactgg ctattgcgcc ttacctcctg ttttgtcttt ttttcattac
      121 caatgtgtgc atgtgaggaa caatatggca ctgcaaaatg agaaaaatag tcgttatctt
      181 ttacgcgact ggaaaccaga aaatccggcc ttctgggaaa ataaaggaaa gcatattgca
      241 cgaagaaacc tctggatatc agtcagttgt ctacttcttg ccttctgtgt ctggatgcta
      301 tttagcgcag ttactgttaa tctcaataaa atcggtttta attttactac cgatcaactc
      361 tttttattaa ccgcattacc ctccgtttct ggcgcattat tgcgtgtccc ctactccttt
      421 atggtgccta tattcggtgg acgccgatgg acggttttta gtactgcaat cctgattatt
      481 ccttgcgtct ggctcggaat tgccgtgcag aatccgaata ctccttttgg gatatttatc
      541 gttatcgctt tgctatgcgg ttttgcaggt gcgaactttg cttcgagcat gggcaatatc
      601 agtttcttct ttccaaaagc caaacaaggg agcgctcttg ggattaatgg cggattagga
      661 aacttaggtg taagtgtaat gcagctggtt gcaccgctgg tcatttttgt ccccgtattt
      721 gcctttctcg gcgtcaatgg cgtaccgcag gccgacggtt cggtgatgtc gctggcgaat
      781 gccgcatgga tttgggtgcc attactggcg attgccacga tcgccgcctg gtcagggatg
      841 aatgatatcg ccagttcacg cgcctcaatt gccgaccagc tccctgtctt acaacgcctg
      901 catctctggc tgctgagcct gctttacctt gccaccttcg gttcgtttat cggtttttct
      961 gcgggttttg ccatgctggc aaaaacccag ttcccggatg tgaatattct gcgcctggcg
     1021 ttctttggcc catttatcgg tgccatcgcg cgatcggttg gtggtgctat ttccgataaa
     1081 ttcggcggcg tgcgggtgac gttgatcaac ttcattttta tggcgatttt cagcgccctg
     1141 ctgttcctta ccttaccggg cacaggctcc ggtaatttca tcgcatttta cgccgtattt
     1201 atggggctgt ttctgaccgc gggtctggga agtggttcta ctttccagat gatcgccgtc
     1261 atctttcgcc agataacaat ttatcgggtg aagatgaaag gcggtagtga tgagcaagct
     1321 caaagagaag ccgtcaccga aacggcagcc gctctgggct ttatctcagc cattggcgca
     1381 gtgggcggct tttttattcc gcaggcgttt ggcatgtcgc tcaatatgac cggctctccg
     1441 gtgggcgcga tgaaagtgtt tttaatcttc tacatcgttt gtgtgctact gacctggctg
     1501 gtttatggtc ggcggaagtt cagccaaaaa taagagatcg atgcctgaca cgtcttatca
     1561 ggctacaacc gtgtcgcatc cgacaaggca tcagatccca attcattcct ggagcaggag
     1621 ttatgtcatg agtaaacttt tggatcgctt tcgctacttc aaacaaaagg gcgaaatctt
     1681 tgccgatggt cacggacagg tgatgcatag caaccgcgac tgggaggaca gctatcgcca
     1741 gcgttggcag ttcgacaaaa tcgtgcgttc cacccacggt gttaactgta caggctcctg
     1801 tagctggaaa atctacgtta aaaatggtct ggtgacctgg gaaatccaac agaccgatta
     1861 cccgcgcact cgccctgacc tgccgaatca cgaacctcgc ggctgcccgc gtggcgcaag
     1921 ttactcctgg tatctctaca gcgcgaaccg cctgaaatac ccgctcattc gtaaacgact
     1981 gattgaactg tggcgcgaag ccctcaagca acacagcgat ccggtactgg cgtgggcatc
     2041 gattatgaac gatccgcaaa agtgcctgag ctacaaacaa gtgcgtggac gcggtggttt
     2101 tatccgctcc aactggcagg agctaaatca gctgattgcc gccgctaacg tctggaccat
     2161 caaaacttac ggcccggatc gcgttgccgg tttctcgccg attccggcga tgtcgatggt
     2221 ttcttacgct gccggaacac gctatctgtc gctgttaggt ggcacctgtt taagcttcta
     2281 cgactggtat tgcgacctgc cgcccgcctc gccgatgacc tggggcgagc aaaccgacgt
     2341 accggaatct gccgactggt ataactccag ttacatcatc gcctgggggt ctaacgtacc
     2401 gcagacacgt acgccggacg cccatttctt taccgaagta cgctacaaag gcactaaaac
     2461 catcgccatt acccctgact actctgaagt ggccaaattg tgcgaccagt ggctggcacc
     2521 gaaacaaggc actgatagtg ccctggcaat ggcaatgggc catgtcattt taaaagagtt
     2581 tcatctcgat aatcccagcg attattttat caactactgc cgccgctaca gcgacatgcc
     2641 gatgctggta atgctggaac ctcgcgacga cggcagctac gttcccgggc gcatggtccg
     2701 cgcatctgac ctggtggatg gactgggcga aagcaataat ccgcagtgga aaaccgtggc
     2761 agttaatacc gcaggtgaat tggtagtgcc gaacggttcg attggttttc gttggggaga
     2821 aaaaggcaaa tggaacctgg aatccattgc cgccggtacg gaaaccgaat tgtcgttaac
     2881 cctgctcggt caacatgacg ccgttgcagg cgtggccttc ccctactttg gaggcattga
     2941 aaatccgcat tttcgcagcg taaaacacaa tcctgtcctg gtgcgccaat tgcccgttaa
     3001 aaatctgaca ttagccgatg gtagcacctg tccggtggtc agcgtttatg atctggtact
     3061 ggcaaattac ggcctcgatc gcgggctgga agatgaaaac agcgcgaaag attacgctga
     3121 aatcaaacct tacacccctg cctggggcga gcaaattacc ggcgtaccgc gccagtatat
     3181 tgaaaccatc gcccgtgaat ttgccgatac cgctcataaa acgcatgggc gctcgatgat
     3241 tattctcggc gcaggtgtta accactggta tcacatggac atgaactacc gcgggatgat
     3301 caatatgctg atcttctgcg gttgtgtcgg gcaaagcggc ggcggctggg cgcactatgt
     3361 cggtcaggaa aaactgcgcc cacaaaccgg ctggttgccg ctggcctttg cgttcgactg
     3421 gaaccgtcca ccgcgccaga tgaacagcac ctcgtttttc tacaatcact ccagccagtg
     3481 gcgctatgaa aaagtcaccg cgcaggaatt gctctcaccg ctcgccgatg ccagtaaata
     3541 cagcggtcac ctgattgatt tcaacgttcg cgccgaacgt atgggctggc tcccttctgc
     3601 gccgcagctg gggcgtaacc cgctctccct gaaagctgaa gccgacaagg ccggattatc
     3661 ccccacagaa tttaccgccc aggcgctgaa atcgggcgat ttacgtatgg cctgcgaaca
     3721 accagatagc ggcagcaatc atccgcgtaa tttgtttgtc tggcgttcta acctgcttgg
     3781 ctcctccggc aaaggccacg agtatatgca gaagtatctg ctggggaccg aaagcgggat
     3841 tcagggagag gaactcggtg ccagcgacgg gatcaaacca gaagaagtcg agtggcaaac
     3901 tgcggcgatt gaaggcaagc tcgacctgct ggtgacgctc gatttccgca tgtccagtac
     3961 ctgcctgttc tccgatatcg ttctgcccac cgccacctgg tacgaaaaag acgatatgaa
     4021 tacctcggat atgcatccgt ttattcatcc gctttctgcg gcggtcgatc cggcgtggga
     4081 atcacgcagc gactgggaaa tctacaaagg tatcgctaaa gccttttcgc aagtgtgcgt
     4141 ggggcatctc ggcaaagaaa ccgacgtggt attacaacca ctgctgcatg actccccggc
     4201 agagctttca cagccgtgtg aagtgctcga ctggcgcaaa ggcgagtgcg atctgattcc
     4261 aggcaaaacc gcgccgaata ttgtggcggt ggagcgcgac taccctgcta cctatgaacg
     4321 ctttacctcg ctcgggccat tgatggacaa acttggcaac ggaggtaaag ggatttcgtg
     4381 gaatacccag gatgaaattg atttcctcgg taaactcaat tacaccaagc gtgatggccc
     4441 ggcacagggg cgtccgctga ttgataccgc gattgatgcc tcagaagtga ttctggcgct
     4501 ggcaccggaa accaacggcc atgttgcagt caaagcgtgg caagcgctgg gcgagatcac
     4561 cggacgcgaa catacccatc tggcgctgca caaagaggac gagaagattc gctttcgcga
     4621 tattcaggcg cagccgcgta aaattatctc cagccccaca tggtctggtc tggaaagcga
     4681 tcacgtctcc tataatgcgg gatacaccaa cgttcatgag ctaattccgt ggcgcacact
     4741 gtcgggccgt caacagcttt atcaggatca tccgtggatg cgtgcctttg gtgaaagcct
     4801 ggtggcttat cgcccgccta tcgacacccg cagtgtcagt gaaatgcgcc aaatcccgcc
     4861 aaacggcttc ccggaaaaag cccttaactt cctgacaccg caccagaaat ggggcattca
     4921 ctcaacctac agtgaaaacc tgctaatgct gacgctctct cgcggtggac cgattgtctg
     4981 gatcagcgaa accgatgccc gtgaactgac cattgtcgat aacgactggg tggaagtgtt
     5041 taacgccaat ggcgcgctga cggcccgcgc ggtggtcagc cagcgtgtac cgccgggcat
     5101 gaccatgatg tatcacgctc aggaacgcat tatgaatatt cctggttcgg aagtaaccgg
     5161 catgcgcggc ggcattcata actcggtcac ccgcgtttgt ccgaaaccaa cacatatgat
     5221 tggcggttac gcgcagctgg cctggggctt taactactac ggcactgtcg gctctaaccg
     5281 cgacgaattc atcatgatcc gcaagatgaa gaacgttaac tggctggatg atgaaggtcg
     5341 cgatcaggtt caggaggcga aaaaatgaaa atacgttcgc aagtcggcat ggtgcttaac
     5401 ctcgataaat gtatcggctg ccatacctgt tcggtgacct gtaaaaacgt ctggaccggt
     5461 cgcgaaggca tggagtacgc atggtttaac aacgtcgaaa ccaaaccggg cattggttat
     5521 ccgaaaaact gggaagatca ggatgagtgg caaggtggct gggtgcgtga tgtgaatggc
     5581 aagatacgcc cgcgtctggg cagcaagatg ggcgtaataa ccaaaatttt cgccaacccg
     5641 gtagtgccgc agattgacga ttactacgaa cctttcacct tcgactacga acatttgcat
     5701 agcgcgccgg aaggcaaaca tattcccacc gcccgtccgc gttcactgat tgacggcaaa
     5761 cggatggaca aagtgatctg gggcccaaac tgggaagaac tgctgggcgg cgaatttgaa
     5821 aaacgagccc gcgaccgcaa cttcgaggcc atgcaaaagg agatgtacgg gcagtttgaa
     5881 aacaccttca tgatgtacct gccgcgcctg tgcgaacact gcctcaatcc cagctgcgtg
     5941 gcgacctgcc ccagcggcgc tatctacaaa cgcgaagaag acggcattgt gctgattgat
     6001 caggataaat gccgtggctg gcgtttgtgc attagcggtt gtccgtacaa aaaaatctac
     6061 ttcaactgga aaagcggcaa gtcagaaaaa tgtatcttct gttatccgcg aattgagtcc
     6121 ggtcagccaa cagtctgctc cgaaacctgc gtgggtcgca tccggtatct gggcgtgctg
     6181 ctctacgacg ccgaccgcat cgaagaagca gcgagtaccg aacgagaggt cgatctctat
     6241 gaacgccagt gcgaagtgtt cctcgatccg cacgatccct cagtgatcga agaagccctg
     6301 aaacaaggta ttccacaaaa cgtgattgac gctgcccagc gttcgcctgt ctacaaaatg
     6361 gcgatggact ggaaactggc gctaccgctg caccctgaat accgcaccct gccgatggtc
     6421 tggtacgttc ctccgctgtc accgattcag tcctacgctg atgcgggagg tttgccgaaa
     6481 agcgaaggcg tgctgcccgc catcgaaagc ctgcgtattc cggtgcaata tctcgccaat
     6541 atgttgagtg ccggtgatac cggtccggta ctgcgggcgc tgaaacggat gatggcgatg
     6601 cgccactata tgcgttcaca aaccgtggaa ggcgttactg atactcgtgc catcgacgaa
     6661 gtaggtctga gcgtcgccca ggtcgaagag atgtatcgtt acctcgccat tgccaactat
     6721 gaagatcgtt tcgtcatccc gacaagccat cgggaaatgg cgggcgatgc cttcgcagaa
     6781 cgcaacggct gcggttttac ctttggcgac ggttgccacg gttcggacag caaattcaac
     6841 ctgttcaaca gtagccgtat cgatgccatc aacatcaccg aagtgcgcga caaagcggag
     6901 ggcgaataat gcagatcctc aaagtgctcg gcctgttgat ggagtaccca gacgagttgt
     6961 tgtgggagtg taaggacgac gcgctggcgc ttatccgccg cgacgcgccg atgctcaccg
     7021 attttgcact cgacctgctc aacgccccgc tgctggataa acaggccgaa tggtgtgaag
     7081 tgtttgaccg cgggcgcacc acgtcgctgc tgctgtttga acatgttcat gccgagtccc
     7141 gcgatcgcgg ccaggcaatg gtggacctgc tggcggagta tgaaaaggtc ggtctgcaac
     7201 tgaattgtag ggaactgccc gattatctac cgctgtatct ggagtattta agcgtacttc
     7261 cggacgatca ggcgaaagaa ggattactca acgttgcgcc gatcctcacc ctgcttggcg
     7321 gacgcttaaa acaacgcgag gcaccgtggt acgcgttatt tgacgccctg ttgcaactgg
     7381 caggaagccc tctgtcaagt gacagtgtca caaaacagat tcgcagtgaa gagcgtgacg
     7441 acacccgcca ggcgctggat gcggtgtggg aagaagaaca ggtgaagttt attgaagata
     7501 acgccacggc gtgtgacagc tcgccgctta atcaatatca gcgacgcttt agccaggatg
     7561 tcgcgccgca gtatgtcgac atcagtgcgg gaggtgggaa atgattcagt atctgaacgt
     7621 ctttttttac gatatctacc cgtacttgtg tggcacggtt tttatccttg gaagctggct
     7681 gcgctacgac tacgggcagt acacctggcg cgccgcctcc agccagatgc tggataaacg
     7741 cgggatggtg ctgtggtcaa acttgttcca tatcggcatt ttggggattt tcttcgggca
     7801 cctgttcggc atgttaacgc cacactggat gtacgcgtgg tttctgccgg tggcggtaaa
     7861 acagcagatg gcgatgatcc tcggcggaat atgcggggta ttgacgctga ttggcggtgc
     7921 ggggctactg gtgcgtcgtt taacaaatcc gcgtatcagg gcgacgtctt ctggtgcgga
     7981 tatcctgatc ctcgccattt tgttaattca gtgcattctc gggttaacca ccattccttt
     8041 ttcagcacaa catccggacg gcagtgaaat gatgaagctg gtgggctggg cgcaaagcat
     8101 tgtcactttc cagggcggtg catctacgca tcttgatggt gtcgccttta tattccgcgt
     8161 tcatctggtg cttggaatga ccatcttcct gctcttcccg ttcacccgac tggtgcatgt
     8221 ctggagcgcg ccgtttgagt actttactcg tcggtatcag attgtgcgtt cgcggcgtta
     8281 attctcacgc gggccggata gcccttcgct ctccggccac ttgcaggcta tgatagttct
     8341 ccagccacag gagaacgtta atgaaaccgc aagtgtacca cgtcgatgct tttacctctc
     8401 aaccgtttcg cggcaattct gccggtgttg ttttccccgc cgataatctc agtgaagcgc
     8461 aaatgcagct tatcgcccgc gagttaggtc attcggaaac tgcttttctg ctgcacagcg
     8521 acgacagcga tgtgcgcatc cgctacttta cgccaacggt tgaagtgcca atttgcggtc
     8581 acgcgacggt ggctgcgcac tatgtacgtt caaaggtatt aggtttggga aattgcacgg
     8641 tctggcaaac gtcgctggca ggaaaacatc gtgtgactat cgaaaagcaa aatgatgatt
     8701 atcgtatttc gctggaacaa ggtacgccag gctttgagcc accactgaca ggtgaaacac
     8761 gtgcagcgat tatcaacgcg cttcatctca ctgaggacga tattctgcca ggcttgccaa
     8821 ttcaggtggc aacaacagga cactcaaaag tgatgatccc gttgaaacca gaagtggata
     8881 tcgacgccct ttcgcctgat cttgctgcgt tgaccgctat cagcaaacag attggctgca
     8941 atggtttctt cccgttccag atccgcccag gcaaaagcga aaccgacggt cgcatgttct
     9001 cgcctgcgat tggtattgtg gaagatccgg tgaccgggaa tgccaatggt ccgatgggcg
     9061 catggttagt acatcacaac gtattgcccc acgatggcaa cgtgttgcgt gttaaaggcc
     9121 atcaggggcg cgcattgggg cgtgatggtg taattgatgt gacggtgaca attcgcgata
     9181 accaaccgga gaaagttacc atttccggca cggcggtgat tctattccat gctgaatggg
     9241 ccattgattt ttgatgccgt tgcctgatgc gcttcgctta tcaggcctac aaccggacta
     9301 atgtaggccg gataaggcat ttacgccaca tccggcaacc tgacataaat tattttcccg
     9361 cctccgggtg cgtatcaaac gccgccatca ccagcgctaa ctcatccaca gtaaagccat
     9421 gtttcgcatc atctacgccc agaccaaact gttcctgcat cacggcatat aacgacgcca
     9481 catctggtag atttcgctgc tcaactgcgt gcccattttc ataatgggta aaatgaaaat
     9541 tggtcagcgt tagcttgcca ccgtccggca aatggcgaca catcagcaaa tgatggcgaa
     9601 aatgcgactg cggccagtgc gccgaccaga aattgcctat cacataatcg ctttgttgct
     9661 gctcgcagag atcaaaacgg tacatcgact gccagtgctg atgatgatta aactgcaata
     9721 cccaatcatc accctcttgc aacaaccgat actctccgtg tggcgtggtt tgcatgatat
     9781 ccggcattaa acgaatcggc gcggttagcg tctgcccacc gaaaccgaca tcagcaatcc
     9841 atttttcccc ttccagttcc accaacagca aacgatgggt gcgcggcggt aatgctggcg
     9901 gatttgataa cactacgcga cctaacaagc tgcgaacgtt aaaccccagc tcgcgtaaca
     9961 cccgctcaaa cacgccattc tgctcaaaac agtaaccgcc acgacgggct atcaccagtt
    10021 tctcttccag cgattgatca tcaagctgta tttccctcgg cagcaaaacg tcgagatttt
    10081 caaacggaat ggtgcaattg tgtttcaggt gcaatgcacg cagcgtatca atattggccg
    10141 cagcagctcc caaccagtta atacgggcaa aataatgatt cagaatgggc gtcatcaggc
    10201 gtttccctta gtggttttct caacttataa cctggaatag acgccgcact ggctattttg
    10261 tgcgatcgcg gacatgcatc acgttcgcaa atcgccaatc ccgttaattt cgttacttat
    10321 tgcgttacga gctacgcttt taaaacaccc ttcatagcga ggcgatatcg tgagccaatt
    10381 cattcccgtc gaattacacc atgccagctg tctgttaaat catggcccaa ctgtcatgat
    10441 caccagtttc gatgagcaat cccagcggcg taacattatg gcagcagcct ggtcgatgcc
    10501 cgtagagttt gaaccgccac gcgtggcgat tgtggtagat aaatcgacat ggaccaggga
    10561 gttgattgaa ggtaacggta aatttggcat cgttatcccg ggcgttgcgg cgactaactg
    10621 gacgtgggcg gtgggaagtg tgtcgggtcg tgaagaagat aagtttaatt gctacggcat
    10681 tccggtagtg agaggcccgg tgcttggttt gccgctggtc gaggaaaaat gtctggcgtg
    10741 gatggagtgt cgcctgctac ctgcgacttc cgcgcaagaa aaatacgata cgctatttgg
    10801 cgaagtggta tctgcagcgg cagacgcacg agtatttgtc gaaggccgct ggcagtttga
    10861 tgatgataag ctcaatacgt tgcatcattt aggtgctggc atgtttgtta ccagtggtaa
    10921 gcgagttacg gcaggttaag cattacatgc tgtaaccggg tttctttatc aaagcctcca
    10981 tttggggcgc gatttcggcc agggcaagat tacgaaagcc cgctccccgc aaggactgac
    11041 gcgagatagt ttctgtccat ggctgctttt cgcatcttac gtcttaaccc tgccttgaat
    11101 accttatcat tcgttaaaat attaatagcg atgtgccgta tccctgaaaa taattctgcg
    11161 gcgtttcctc ttcttatttt gcagtcgtct tcattcatta ccacgtccag acgccagtgc
    11221 agcttattct ccacgtgcca gtggtttcgg attgctgtgg cgaacttctc tgcggttaaa
    11281 tcagcagaac tgatataata tctgaccgtc atttctggct ctttcttttg ttctgctatt
    11341 atggaccgaa aggagactgc cacgcataat ttcttcagtc ctttccattc gaacgtgaaa
    11401 tcaataagtt catcagggac atcgcaaaca atatgaagac ggatttcttc tctgccgtga
    11461 ctcttttcac taatggcgta actgtcatgt tctggattat ttaattcttt cagtggaaat
    11521 ttttcctcaa aggctttatt aagccgcccc tgatttcctt ttacagcgaa taaataatca
    11581 ccgccctgtt tttgtatctt ctctgcaata tctttctggc aacccatcgc atcagttgtg
    11641 atgatttttc ctttaatatc cagcatgtta agaagttcag ggataggtgt aatttcattg
    11701 gatttctcat ccgtcctgat ctgtccgatg accagactgt gcattgttga gaacgcacta
    11761 atgacatgaa tcgctccctt gcggcgactc ttgtcataag aatgccggag tgtttttcca
    11821 tcaattgcga tgacgtcttt atcatctgaa gaatggcagt cacgcatcca gttaataaag
    11881 cactcgtgaa attttgcagg actgatacag gatacaactc tggcaatggt atcgtgaaca
    11941 ggaataccat tatcaaaatc accatattgc ttcaaaaaat cgagatgtgt ttccccaaaa
    12001 tcctctatat cttcccagcc ttctgcaccc gaaataacgg cacaaatagt caacagtaga
    12061 atatccgata att
//
LOCUS       DAHHTG010000124        12001 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.125, whole genome shotgun sequence.
ACCESSION   DAHHTG010000124 DAHHTG010000000
VERSION     DAHHTG010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12001)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12001)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12001
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.125"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            110..979
                     /locus_tag="NBI74_003617"
     CDS             110..979
                     /locus_tag="NBI74_003617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjeJ family protein"
                     /protein_id="HCD1933895.1"
                     /translation="MAISIKGVNTGVIRKSNNFIALALKIKEPRNKESLFFMSAMELR
                     DLLIALESRLHQKHKLDAAARLQYEQARDKVIKKMAENIPEILVDELKNADINRRVNT
                     LELTDDQGENLTFVLTLHDGSKCELVVNELQIEMLARAIIHAINNAEMRELALRITSL
                     LDFLPLYDVDCQDDGNLEYDTYSQPEWKHNLFNHYLAVLYRFKDESGKEQFSGAVVKT
                     REATPGKEVEAITRRMLDFSPRLKKLAGVPCQVYVRTVAANNAQPLTQDQCLRALHHL
                     RVQSTSKTAPQAK"
     gene            complement(1029..1382)
                     /locus_tag="NBI74_003618"
     CDS             complement(1029..1382)
                     /locus_tag="NBI74_003618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4156 domain-containing protein"
                     /protein_id="HCD1933896.1"
                     /translation="MHVKYLAGIVGAALLMAGCSSSNELSAAGQSVRIVDEQPGAECQ
                     LIGTATGKQSNWLSGQHGEEGGSMRGAANDLRNQAAAMGGNVIYGISSPSQGMLSSFV
                     PTDSQIIGQVYKCPN"
     gene            complement(1520..3166)
                     /gene="groL"
                     /locus_tag="NBI74_003619"
     CDS             complement(1520..3166)
                     /gene="groL"
                     /locus_tag="NBI74_003619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120975.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HCD1933897.1"
                     /translation="MAAKDVKFGNDARVKMLRGVNVLADAVKVTLGPKGRNVVLDKSF
                     GAPTITKDGVSVAREIELEDKFENMGAQMVKEVASKANDAAGDGTTTATVLAQAIITE
                     GLKAVAAGMNPMDLKRGIDKAVTAAVEELKALSVPCSDSKAIAQVGTISANSDETVGK
                     LIAEAMDKVGKEGVITVEDGTGLQDELDVVEGMQFDRGYLSPYFINKPETGAVELESP
                     FILLADKKISNIREMLPVLEAVAKAGKPLLIIAEDVEGEALATLVVNTMRGIVKVAAV
                     KAPGFGDRRKAMLQDIATLTGGTVISEEIGMELEKATLEDLGQAKRVVINKDTTTIID
                     GVGEEAAIQGRVAQIRQQIEEATSDYDREKLQERVAKLAGGVAVIKVGAATEVEMKEK
                     KARVEDALHATRAAVEEGVVAGGGVALIRVASKLADLRGQNEDQNVGIKVALRAMEAP
                     LRQIVLNCGEEPSVVANTVKGGDGNYGYNAATEEYGNMIDMGILDPTKVTRSALQYAA
                     SVAGLMITTECMVTDLPKNDAADLGAAGGMGGMGGMGGMM"
     gene            complement(3210..3503)
                     /locus_tag="NBI74_003620"
     CDS             complement(3210..3503)
                     /locus_tag="NBI74_003620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HCD1933898.1"
                     /translation="MNIRPLHDRVIVKRKEVETKSAGGIVLTGSAAAKSTRGEVLAVG
                     NGRILENGEVKPLDVKVGDIVIFNDGYGVKSEKIDNEEVLIMSESDILAIVEA"
     gene            3779..5035
                     /gene="yjeH"
                     /locus_tag="NBI74_003621"
     CDS             3779..5035
                     /gene="yjeH"
                     /locus_tag="NBI74_003621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine/branched-chain amino acid
                     transporter"
                     /protein_id="HCD1933899.1"
                     /translation="MSGLKQELGLAQGIGLLSTSLLGTGVFAVPALAALVAGNNSLWA
                     WPVLIILVFPIAIVFAILGRHYPSAGGVAHFVGMAFGSRLERVTGWLFLSVIPVGLPA
                     ALQIAAGFGQAMFGWHSWQLLLAELGTLALVWYIGTRGASSSANLQTVIAGLIVALIV
                     AIWWAGDIKPTNIPFPAPGNIELTGLFAALSVMFWCFVGLEAFAHLASEFKNPERDFP
                     RALMIGLLLAGLVYWGCTVVVLHFDAYGEQMAAAASLPKIVVQLFGVGALWIACVIGY
                     LACFASLNIYIQSFARLVWSQAQHNPDHYLARLSSRHIPNNALNAVLGCCVVSTLVIH
                     ALGINLDALIIYANGIFIMIYLLCMLAGCKLLQGRYRLLAVVGGLLCVLLLAMVGWKS
                     LYALIMLAGLWLFLPKRKTPENGITT"
     gene            complement(5051..5527)
                     /gene="fxsA"
                     /locus_tag="NBI74_003622"
     CDS             complement(5051..5527)
                     /gene="fxsA"
                     /locus_tag="NBI74_003622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein FxsA"
                     /protein_id="HCD1933900.1"
                     /translation="MRWLPFIAIFLYVYIEISIFIQVAHVLGVLLTLVLVIFTSVIGM
                     SLVRNQGFKNFVLMQQKMAAGENPAAEMIKSVSLIIAGLLLLLPGFFTDFLGLLLLLP
                     PVQKHLTVKLMPHLRFSRMPGGGFSAGTGGGNTFDGEYQRKDDDRDRLDHKDDRQD"
     gene            5864..7300
                     /gene="aspA"
                     /locus_tag="NBI74_003623"
     CDS             5864..7300
                     /gene="aspA"
                     /locus_tag="NBI74_003623"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313147.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HCD1933901.1"
                     /translation="MSNNIRIEEDLLGTREVPADAYYGVHTLRAIENFYISNNKISDI
                     PEFVRGMVMVKKAAAMANKELQTIPKSVANAIIAACDEVLNNGKCMDQFPVDVYQGGA
                     GTSVNMNTNEVLANIGLELMGHQKGEYQYLNPNDHVNKCQSTNDAYPTGFRIAVYSSL
                     IKLVDAINQLREGFERKAVEFQDILKMGRTQLQDAVPMTLGQEFRAFSILLKEEVKNI
                     QRTAELLLEVNLGATAIGTGLNTPKEYSPLAVKKLAEVTGFPCVPAEDLIEATSDCGA
                     YVMVHGALKRLAVKMSKICNDLRLLSSGPRAGLNEINLPELQAGSSIMPAKVNPVVPE
                     VVNQVCFKVIGNDTTVTMAAEAGQLQLNVMEPVIGQAMFESVHILTNACYNLLEKCIN
                     GITANKEVCEGYVYNSIGIVTYLNPFIGHHNGDIVGKICAETGKSVREVVLERGLLTE
                     AELDDIFSVQNLMHPAYKAKRYTDESEQ"
     gene            7418..8719
                     /gene="dcuA"
                     /locus_tag="NBI74_003624"
     CDS             7418..8719
                     /gene="dcuA"
                     /locus_tag="NBI74_003624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuA"
                     /protein_id="HCD1933902.1"
                     /translation="MLVVELIIVLLAIFLGARLGGIGIGFAGGLGVLVLAAIGVKPGN
                     IPFDVISIIMAVIAAISAMQVAGGLDYLVHQTEKLLRRNPKYITILAPIVTYFLTIFA
                     GTGNISLATLPVIAEVAKEQGVKPCRPLSTAVVSAQIAITASPISAAVVYMSSVMEGH
                     GISYLHLLSVVIPSTLLAVLVMSFLVTMLFNSKLSDDPIYRKRLEEGLVELRGEKQIE
                     IKSGAKTSVWLFLLGVVGVVIYAIINSPSMGLVEKPLMNTTNAILIIMLSVATLTTVI
                     CKVDTDNILNSSTFKAGMSACICILGVAWLGDTFVSNNIDWIKDTAGEVIQGHPWLLA
                     VIFFFASALLYSQAATAKALMPMALALNVSPLTAVASFAAVSGLFILPTYPTLVAAVQ
                     MDDTGTTRIGKFVFNHPFFIPGTLGVALAVCFGFVLGSFML"
     gene            8835..9173
                     /gene="cutA"
                     /locus_tag="NBI74_003625"
     CDS             8835..9173
                     /gene="cutA"
                     /locus_tag="NBI74_003625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710002.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent cation tolerance protein CutA"
                     /protein_id="HCD1933903.1"
                     /translation="MLDEKSSNTTSVVVLCTAPDEATAQDLAAKVLAEKLAACATLIP
                     GATSLYYWEGKLEQEYEVQMILKTTVSHQQALLECLKSHHPYQTPELLVLPVTHGDTD
                     YLSWLNASLR"
     gene            9149..10846
                     /gene="dsbD"
                     /locus_tag="NBI74_003626"
     CDS             9149..10846
                     /gene="dsbD"
                     /locus_tag="NBI74_003626"
                     /EC_number="1.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HCD1933904.1"
                     /translation="MAQRIFTLILLLCSTSVFAGLFDAPGRSQFVPADQAFAFDFQQN
                     QHDLNLTWQIKDGYYLYRKQIRITPEHAKIADVQLPQGVWHEDEFYGKSEIYRDRLTL
                     PVTINQASAGATLTVTYQGCADAGFCYPPETKTVPLSEVVANNEASQPVSVPQQEQPT
                     AQLPFSALWALLIGIGIAFTPCVLPMYPLISGIVLGGKQRLSTARALLLTFIYVQGMA
                     LTYTALGLVVAAAGLQFQAALQHPYVLIGLAIVFTLLAMSMFGLFTLQLPSSLQTRLT
                     LMSNRQQGGSPGGVFIMGAIAGLICSPCTTAPLSAILLYIAQSGNMWLGGGTLYLYAL
                     GMGLPLMLITVFGNRLLPKSGPWMEQVKTAFGFVILALPVFLLERVIGDIWGLRLWSA
                     LGVAFFGWAFITSLQAKRGWMRVVQIILLAAALVSVRPLQDWAFGATHTAQTQTHLNF
                     TQIKTVDELNQALVEAKGKPVMLDLYADWCVACKEFEKYTFSDPQVQKALADTVLLQA
                     NVTANDAQDVALLKHLNVLGLPTILFFDGQGQEHPQARVTGFMDAETFSAHLRDRQP"
     gene            10883..11458
                     /locus_tag="NBI74_003627"
     CDS             10883..11458
                     /locus_tag="NBI74_003627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCD1933905.1"
                     /translation="MQREDVLGEALKLLELQGIANTTLEMVAERVDYPLDELRRFWPD
                     KEAILYDALRYLSQQIDVWRRQLMLDETQTAEQKLLARYQALSECVKNNRYPGCLFIA
                     ACTFYPDPGHPIHQLADQQKSAAYDFTHELLTTLEVDDPAMVAKQMELVLEGCLSRML
                     VNRSQADVDTAHRLAEDILRFARCRQGGALT"
     gene            11565..11640
                     /locus_tag="NBI74_003628"
     tRNA            11565..11640
                     /locus_tag="NBI74_003628"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11598..11600,aa:Phe,seq:gaa)
BASE COUNT         2901 a         3097 c         2941 g         3062 t
ORIGIN      
        1 tgagcacggc tatttatgtt attttaacaa ctattactac caagcccatt tatctggctt
       61 ataaactaaa attaattatt taattgagaa atggttaggg agaacctaca tggccataag
      121 catcaagggt gttaataccg gtgttattcg caagagtaat aatttcattg cactggcgct
      181 gaaaattaaa gaacctcgca ataaagaatc gttgttcttt atgtctgcca tggaattgcg
      241 cgaccttctg attgcgctgg aaagtcgtct gcatcaaaag cataaactgg atgctgctgc
      301 acgccttcag tatgaacaag ctcgcgataa agtcattaag aaaatggcag aaaatatccc
      361 agaaattctg gttgatgagc ttaaaaatgc cgatattaat cgtcgggtta acacgctgga
      421 acttaccgat gatcagggcg aaaatcttac cttcgtatta acactacatg acggtagcaa
      481 atgtgagtta gtcgtcaatg agttgcagat tgaaatgctg gcgcgagcaa tcattcatgc
      541 aattaataat gcggaaatgc gtgaactggc attacgtatt acctcactgt tagatttcct
      601 gccgttatat gatgtcgact gccaggatga tggtaatctg gaatatgaca cctattccca
      661 accggaatgg aaacataact tgttcaacca ttatctggcg gtgctttacc gctttaaaga
      721 tgaaagtggc aaagaacaat ttagcggcgc agtggtgaaa acgcgtgaag caacgccggg
      781 taaagaagtg gaggcgatca ctcgccgaat gcttgatttc agcccccgac tgaaaaaact
      841 cgcgggtgtg ccatgtcagg tctatgttcg caccgtagcg gcaaataacg cccaaccgct
      901 aacccaggat caatgcttac gcgcgctgca tcacttacgt gttcaatcca ccagcaaaac
      961 tgcaccacag gcgaaataat cttatctgtt ttactacgag gctggaagta tccagcctct
     1021 ggatcacatc agttcgggca cttataaacc tgaccgataa tctggctatc cgtcgggaca
     1081 aaactggaca acattccctg cgacgggctg ctgatgccat aaatcacgtt accgcccatt
     1141 gcagccgcct ggttgcgcag atcgtttgct gcgccgcgca tagaaccgcc ttcttctccg
     1201 tgttgcccgg aaagccagtt gctttgctta cctgttgcag taccaatcag ctggcactct
     1261 gcacctggtt gctcgtccac aatgcgtaca ctctgaccgg cagcactcaa ttcgttgctg
     1321 gagctacaac ccgccatcag tagcgcggca ccgacaatcc ctgctaagta ttttacgtgc
     1381 atgttattcc ccataatcaa tgagctggac gccactcgcg tcgtccgtgt ctgaatctta
     1441 tactagaaag atgaccaaaa gaaaaacccc cagacatttc tgcccggggg tttgtttatt
     1501 tctgcgaggt gcagggcaat tacatcatgc cgcccatgcc acccatgccg cccataccgc
     1561 cagcagcgcc taagtcagct gcatcgtttt tcggcaggtc ggtaaccatg cactcggtgg
     1621 tgatcatcag gccagccaca gaagccgcgt actgcagagc agaacgggtt actttggttg
     1681 gatccaggat acccatatcg atcatgttgc cgtattcttc agttgctgcg ttgtaaccgt
     1741 agttgccgtc gccgccttta acggtgttag caacaacaga cggttcttcg ccgcagttca
     1801 ggacgatctg acgcagcgga gcttccattg cacgcagtgc aactttgata cccacgttct
     1861 ggtcttcgtt ctgaccacgc aggtcagcca gtttagacgc tacgcggatc agcgcaacac
     1921 caccaccagc aaccacgcct tcttctaccg cagcacgggt cgcgtgcagg gcgtcttcaa
     1981 cgcgtgcttt tttctctttc atttcaactt cggtagcagc accaactttg ataactgcaa
     2041 cgccgcctgc cagtttcgct acgcgctcct gcagtttttc acggtcgtag tcagaagttg
     2101 cttcttcaat ctgctgacgg atctgagcaa cacggccctg gattgcagct tcttcaccca
     2161 cgccatcgat gatggtggtg gtgtctttgt tgatcacaac acgtttagcc tgacccaggt
     2221 cttccagggt tgctttttcc agctccatac cgatctcttc agagattacg gtaccgccag
     2281 tcagggttgc gatatcctgc agcatagctt tacgacgatc gccgaagccc ggagctttaa
     2341 ccgcagcaac tttcacgatg ccacgcatgg tgttaacaac cagagttgcc agtgcttcgc
     2401 cttcaacatc ttcagcgatg atcagcagcg gtttacctgc tttggcaaca gcttccagaa
     2461 ccggcagcat ttcgcggatg ttggagattt tcttgtcagc cagcaggatg aacgggcttt
     2521 ccagttctac tgcgccagtt tccggcttgt tgatgaagta aggagacaag tagccacggt
     2581 cgaactgcat accttcaacc acgtccagtt cgtcctgcag accggtaccg tcttcaacgg
     2641 tgataacgcc ttctttaccg actttgtcca tcgcttcagc gatcagttta cctacggttt
     2701 cgtcggagtt agcagagatg gtaccaacct gagcaatcgc tttagagtca gagcacggta
     2761 cggacagcgc tttcagttct tcaactgcag cggtaacagc tttgtcgata ccacgtttca
     2821 ggtccatcgg gttcatgccc gcagcaacag ctttcagacc ctcagtgatg atagcctgag
     2881 ccagtacggt tgcagtggtg gtaccgtcgc ctgcagcgtc attcgcttta gaggcaactt
     2941 ctttcaccat ctgcgcaccc atattttcga acttgtcttc cagttcgatt tcacgagcaa
     3001 cggaaacacc atctttggtg atggtcggtg caccgaaaga tttatccaga actacgttac
     3061 ggcctttagg accgagggta actttcactg catctgccag tacgtttacg ccgcgcagca
     3121 ttttcacacg agcgtcgtta ccgaatttta cgtctttagc tgccattatc tttattcctt
     3181 aaattcgtat gttcagtgtc gtgcgcggat tacgcttcaa caattgccag aatgtcgctt
     3241 tcggacatga tcaacacttc ttcattgtcg atcttctcag atttcacacc gtagccatcg
     3301 ttgaaaataa cgatgtcgcc aactttcaca tccagcggct tcacttcgcc attttcaagg
     3361 atacggccat tgccgacagc cagcacttcg ccgcgggtgg atttagccgc tgcagagccg
     3421 gtcagaacga tgccgccagc agatttagtt tcaacttctt tacgcttgac gatcacgcga
     3481 tcatgcaatg gacgaatatt cattgataac tctcctttga gaaagtccgt atctgttatg
     3541 ggtgacgccg gagcttacgt ggtttcccgg ctggtgacca gagaaatggg gatgaggctt
     3601 cgccccttca agggggaaaa agaaaaaaaa ttctgatcag cacaaaatcg ggtgaaaacc
     3661 ctgattcacc tcacatttca tcgctatttc ttcatcaccg tcataagcaa atctgattgt
     3721 gctaccatcg aaaatctacg cagttgccca aaatttgggc gcaatcggac atcaacccat
     3781 gagtggactc aaacaagaac tggggctggc ccagggcatc ggcctgctat cgacgtcatt
     3841 attaggcact ggcgtgtttg ccgttcctgc gttagctgcg ctagtagcag gcaataacag
     3901 cctgtgggcg tggcccgttt tgattatctt agtgttcccg attgcgattg tgtttgcgat
     3961 tctgggtcgc cactatccca gcgcaggcgg cgtcgcgcac ttcgtcggta tggcgtttgg
     4021 ttcgcggctt gagcgagtca ccggctggtt gtttttatcg gtcattcccg tgggtttgcc
     4081 tgccgcgcta caaattgctg ccggattcgg ccaggcaatg tttggctggc atagctggca
     4141 actgttgttg gcagaactcg gtacgctggc gctggtgtgg tatatcggta ctcgcggtgc
     4201 cagttccagt gctaatctac aaacagttat tgccggactt atcgtcgcac tgattgtcgc
     4261 tatctggtgg gcgggcgata tcaaacctac gaatatcccc ttccctgcgc caggaaatat
     4321 cgaacttacc gggttatttg ctgcgttatc agtgatgttc tggtgttttg tcggtctgga
     4381 agcatttgcc catcttgcct cggaatttaa aaatccagag cgtgattttc ctcgtgcttt
     4441 gatgattggc ctgctgctgg caggattagt ctattggggc tgtacggtag tcgtcttaca
     4501 cttcgacgcc tatggtgaac aaatggcggc ggcagcatcg cttcccaaaa ttgtagtgca
     4561 gttgttcggt gtaggagcgt tatggattgc ctgcgtaatt ggctatctgg cctgctttgc
     4621 cagtctcaac atttatatac agagcttcgc ccgcctggtc tggtcgcagg cgcaacataa
     4681 tcctgaccac tacctggcac gcctctcttc tcgccatatc ccgaataatg ccctcaatgc
     4741 ggtgctcggc tgctgcgtgg tgagcacgtt ggtgattcat gctttaggga tcaatctgga
     4801 cgcacttatc atctatgcca atggcatctt tattatgatt tatctgttat gcatgctggc
     4861 tggctgtaaa ttattgcaag gacgttatcg actactggcg gtggttggcg ggctgttatg
     4921 cgttctgtta ctggcaatgg tcggctggaa aagtctctac gcgctgatca tgctggcggg
     4981 gttatggctg tttctgccaa aacgaaaaac gccggaaaat ggcataacca cataatccgg
     5041 cgtttcgaca ttaatcctgg cgatcgtctt tatgatcaag gcggtcgcgg tcatcatcct
     5101 ttcgctggta ctcaccatca aaagtattac cgccaccggt cccggcgcta aaaccgccgc
     5161 ctggcatgcg agaaaagcgc aaatgcggca tcaacttcac tgtcagatgc ttttgcaccg
     5221 gcggcaataa aagcagaaga ccgaggaagt cggtgaaaaa gcccggtaat aaaagcagca
     5281 aaccggcaat gatcagcgaa acacttttaa tcatctccgc cgctgggttt tcacccgccg
     5341 ccattttttg ctgcatcagc acgaaattct taaagccctg gttacgtacc agtgacatac
     5401 cgataactga cgtgaatata accagcacga gggtcagcaa tacccccaat acatgagcaa
     5461 cctgaataaa gattgaaatc tcaatataga cataaaggaa aatggcaata aaaggtaacc
     5521 agcgcaaagg tttctcctgt aatagcagcc ggttaaaccc ggctacctga atgggttgcg
     5581 aatcgcgttt agcttatatt gtggtcatta gcaaaatttc aagatgtttg cgcaactatt
     5641 tttggtagta atcccaaagc ggtgatccat ttcacaaatt aataattaag gggtaaaaac
     5701 cgacacttaa agtgatccag gttacggtag aaatcctcaa gcagcatatg atctcgggta
     5761 ttcggtcgat gcaggggata atcgtcggtc gaaaaacatt cgaaaccaca tatattctgt
     5821 gtgtttaaag caaatcattg gcagcttgaa aaagaaggtt cacatgtcaa acaacattcg
     5881 tatcgaagaa gatctgttgg gtaccaggga agttccagct gatgcctact atggtgttca
     5941 cactctgaga gcgattgaaa acttctatat cagcaacaac aaaatcagtg atattcctga
     6001 atttgttcgc ggtatggtaa tggttaaaaa agccgcagct atggcaaaca aagagctgca
     6061 aaccattcct aaaagtgtag cgaatgccat cattgccgca tgtgatgaag tcctgaacaa
     6121 cggaaaatgc atggatcagt tcccggtaga cgtctaccag ggcggcgcag gtacttccgt
     6181 aaacatgaac accaacgaag tgctggccaa tatcggtctg gaactgatgg gtcaccagaa
     6241 aggtgaatat cagtacctga acccgaacga ccatgttaac aaatgtcagt ctactaacga
     6301 cgcctacccg accggtttcc gtatcgcagt ttactcttcc ctgattaaac tggtagatgc
     6361 gattaaccaa ctgcgtgaag gctttgaacg taaagctgtc gaattccagg acatcctgaa
     6421 aatgggtcgt acccagctgc aggacgcagt accgatgacc ctcggtcagg aattccgcgc
     6481 tttcagcatc ctgctgaaag aagaagtgaa aaacatccaa cgtaccgctg aactgctgct
     6541 ggaagttaac cttggcgcaa cagcaatcgg tactggtctg aacacgccga aagagtactc
     6601 tccgctggca gtgaaaaaac tggctgaagt cactggcttc ccatgcgtac cggctgaaga
     6661 cctgatcgaa gcgacctccg actgcggcgc ttatgttatg gttcacggcg cgctgaaacg
     6721 cctggctgtg aagatgtcca aaatctgtaa cgacctgcgc ttgctctctt ctggtccacg
     6781 tgccggcctg aacgagatca acctgccgga actgcaggca ggctcttcca tcatgccagc
     6841 taaagtaaac ccggttgttc cggaagtggt taaccaggta tgcttcaaag tcatcggtaa
     6901 cgacaccact gttaccatgg cagcagaagc aggtcagctg cagttgaacg ttatggagcc
     6961 agtcattggc caggccatgt tcgaatccgt tcacattctg accaacgctt gctacaacct
     7021 gctggaaaaa tgcattaacg gcatcactgc taacaaagaa gtgtgcgaag gttacgttta
     7081 caactctatc ggtatcgtta cttacctgaa cccgttcatc ggtcaccaca acggtgacat
     7141 cgtgggtaaa atctgtgccg aaaccggtaa gagtgtacgt gaagtcgttc tggaacgcgg
     7201 tctgttgact gaagcggaac ttgacgatat tttctccgta cagaatctga tgcacccggc
     7261 ttacaaagca aaacgctata ctgatgaaag cgaacagtaa tcgtacaggg tagtacaaat
     7321 aaagaaggca cgtcagatgg cgtgcctttt ttcttgtgag cagtaactta aaaataacaa
     7381 tttaatatca acttgttaaa aaacaaggaa ggctaatatg ctagttgtag aactcatcat
     7441 agttttgctg gcgatcttct tgggcgccag attgggggga ataggtattg gttttgcagg
     7501 cggattgggg gtgctggttc ttgccgctat tggcgttaaa cccggtaaca tcccgttcga
     7561 tgtcatttcc attatcatgg cggttatcgc cgctatttct gccatgcagg ttgctggcgg
     7621 tctggactat ctggttcatc agacagaaaa gctgctgcgc cgtaacccga aatacatcac
     7681 gatcctcgca ccgatcgtga cctatttcct gactatcttt gctggtactg gcaacatctc
     7741 tctggcgaca ctgccagtta tcgctgaagt tgcgaaggaa caaggcgtta aaccttgccg
     7801 tccgctgtct actgcagtgg tatccgcgca gattgcgatc accgcatcgc caatctcagc
     7861 ggcagtggtt tacatgtctt ccgtgatgga aggtcatggc atcagctacc tccatctgct
     7921 ctccgtggtc atcccgtcca ccctgctggc ggttctggtg atgtccttcc tggtcactat
     7981 gctgttcaac tccaaactct ctgacgatcc gatttatcgc aagcgtctgg aagaaggcct
     8041 ggttgaactg cgcggtgaaa agcagattga aatcaaatcc ggtgcaaaaa cgtccgtctg
     8101 gctgttcctg ctgggcgtag ttggcgtggt tatctatgca atcatcaaca gcccaagcat
     8161 gggtctggtt gaaaaaccac tgatgaacac caccaacgca atcctgatca tcatgctcag
     8221 cgttgcaact ctgaccaccg ttatctgtaa agtcgatacc gacaacattc tcaactccag
     8281 caccttcaaa gcaggtatga gcgcctgtat ttgtatcctg ggtgttgcgt ggctgggcga
     8341 tactttcgtt tccaacaaca tcgactggat caaagatacc gctggtgaag tgattcaggg
     8401 tcatccgtgg ctgctggccg tcatcttctt ctttgcttct gctctgctgt actctcaggc
     8461 tgcaaccgca aaagcactga tgccgatggc tctggcactg aacgtttctc cgctgaccgc
     8521 tgttgcttct tttgctgcgg tgtctggtct gttcattctg ccgacctacc cgacgctggt
     8581 tgctgcggta cagatggatg acacgggtac tacccgtatc ggtaaattcg tcttcaacca
     8641 tccgttcttc atcccgggta ctctgggtgt tgccctggcc gtttgcttcg gcttcgtgct
     8701 gggtagcttc atgctgtaat gacccatcgc ggggcgttca cgccccgctt tctttcccgc
     8761 cgactaacat cctttccccg tccgttgtat agtgacctct ctcttgcggt tccatctgtt
     8821 cttgcgaggt gtttatgctt gatgaaaaaa gttcgaatac cacgtctgtc gtggtgctat
     8881 gtacggcacc ggatgaagcg acagcccagg atttagccgc caaagtgctg gcggaaaaac
     8941 tggcggcctg cgcgaccttg atccccggcg ctacctctct ctattactgg gaaggtaagc
     9001 tggagcaaga atacgaagtg cagatgattt taaaaactac cgtatctcac cagcaggcac
     9061 tgctggaatg cctgaagtct catcatccat atcaaacccc ggaacttctg gttttacctg
     9121 ttacacacgg agacacagat tacctctcat ggctcaacgc atctttacgc tgatcctgct
     9181 actttgcagc acttccgttt ttgccggatt attcgatgcg ccgggacgtt cacaatttgt
     9241 ccccgcggat caagcctttg cttttgattt tcagcaaaac caacatgatc tgaatctgac
     9301 ctggcagatc aaagacggtt actacctcta ccgtaaacag atccgcatta cgccggaaca
     9361 cgcgaaaatt gccgacgtgc agctgccgca aggcgtctgg catgaagatg agttttacgg
     9421 caaaagcgag atttaccgcg atcggctgac gcttcccgta accatcaacc aggcgagtgc
     9481 gggagcaacg ttaactgtca cctaccaggg ctgtgctgat gccggtttct gttatccgcc
     9541 agaaaccaaa accgttccgt taagcgaagt ggtcgccaac aacgaagcgt cacagcctgt
     9601 gtctgttccg cagcaagagc agcccaccgc gcaattgccc ttttccgcgc tctgggcgtt
     9661 gttgatcggt attggtatcg cctttacgcc atgcgtgctg ccaatgtacc cactgatttc
     9721 tggcatcgtg ctgggcggta aacagcggct ttccactgcc agagcattgt tgctgacctt
     9781 tatttatgtg caggggatgg cgctgactta cacggcgctg ggtctggtgg ttgccgccgc
     9841 agggttacag ttccaggcgg cgctacagca cccatacgtg ctcattggcc tcgccatcgt
     9901 ctttaccttg ctggcgatgt caatgtttgg cttgtttact ctgcaactcc cctcttcgct
     9961 gcaaacacgt ctcacgctga tgagcaatcg ccaacagggc ggctcacctg gcggtgtgtt
    10021 tattatgggg gcgattgccg gactgatctg ttcaccatgc accactgcac cgcttagcgc
    10081 gattctgctg tatatcgccc aaagcgggaa catgtggctg ggcggcggca cgctttatct
    10141 ttatgcgctg ggcatgggcc tgccgctgat gctaattacc gtctttggta accgcttgct
    10201 gccgaaaagc ggcccgtgga tggaacaagt caaaaccgcg tttggttttg tgatcctcgc
    10261 actgccggtc ttcctgctgg agcgagtgat tggtgatata tggggattac gcttgtggtc
    10321 ggcgcttggt gtcgcattct ttggctgggc ctttatcacc agcctacagg ccaaacgcgg
    10381 ctggatgcgc gtggtgcaaa taatcctgct ggcagcggca ttggttagcg tacgcccact
    10441 tcaggattgg gcatttggtg caacacatac cgcgcaaact cagacgcatc tcaactttac
    10501 acaaatcaaa acggtagatg agttaaatca ggcgctcgtt gaagccaaag gcaaaccagt
    10561 gatgttagat ctctatgccg actggtgcgt cgcctgtaaa gagtttgaga aatacacctt
    10621 cagcgacccg caggtgcaaa aagcgttagc agatacggtc ttacttcagg ccaacgtcac
    10681 tgccaacgac gcacaagatg tggcgctgtt aaagcatctt aatgtccttg gcctaccgac
    10741 aattctcttt tttgacggac aaggccagga gcatccacaa gcacgcgtca cgggctttat
    10801 ggatgctgaa accttcagcg cacatttgcg cgatcgccaa ccgtgaacga cacttgcagt
    10861 gggaaagacg gaggaaaata gcgtgcaacg tgaagatgta ctgggagaag ccctgaaatt
    10921 attagaatta caagggattg ccaacaccac gctggagatg gttgctgaac gtgtggatta
    10981 tccactggac gagctacgcc gcttctggcc agacaaagag gcaatcctct acgatgcgct
    11041 gcgttatctt agccaacaga tagatgtctg gcgtcgtcag ctgatgctgg acgaaacgca
    11101 aaccgccgaa caaaagttgc tggcacgtta tcaggcgtta tcggagtgcg ttaaaaacaa
    11161 ccgctatccg ggctgtctgt ttatcgctgc ctgtacgttt tatcccgatc ctggccaccc
    11221 tattcatcaa ctggccgatc agcaaaaaag cgcggcctac gatttcaccc acgaactgtt
    11281 aaccacgctg gaagttgacg atccggcgat ggtagcaaag cagatggaac tggtgctgga
    11341 aggctgttta agccgaatgc tggtgaatcg tagccaggcg gatgtcgaca ccgcacatcg
    11401 gctggcggaa gatatcctgc gcttcgcccg ctgccgtcag ggtggtgcac tgacctgaca
    11461 gaaacacaga aaagaagcga tttgccgcaa tcttaagcag ttgaatcgct tttactgaaa
    11521 ttaggttgac gagatgtgca gattacggtt taatgcgccc cgttgcccgg atagctcagt
    11581 cggtagagca ggggattgaa aatccccgtg tccttggttc gattccgagt ccgggcacca
    11641 aattcatata aacggacctc cacggaggtc cgtttttcgt ttcaggacgc cacgatttaa
    11701 gcgtcctgcc gccaaatcaa ttctaccgaa ctcaaccaga ttctccccac atcaccagca
    11761 atttgcgggc atatcccaat tcgggaaaat ttgtttctga gctatagcgc tgactgacgt
    11821 gaaatgtcgt gcggccccgt gatgctgttg aacgtcaaat gacgtcatca ggagcgtaac
    11881 gcatccataa agcacaacat cgggcagaac gccaactgat gagattttct gaatgagaac
    11941 aaagagaaat gtatcagtcc gtttactcat gcaaagacta acaatccatt aaaatagtaa
    12001 g
//
LOCUS       DAHHTG010000125        11982 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.126, whole genome shotgun sequence.
ACCESSION   DAHHTG010000125 DAHHTG010000000
VERSION     DAHHTG010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11982)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11982)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11982
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.126"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            complement(<1..1355)
                     /gene="uidB"
                     /locus_tag="NBI74_003629"
     CDS             complement(<1..1355)
                     /gene="uidB"
                     /locus_tag="NBI74_003629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide transporter"
                     /protein_id="HCD1933906.1"
                     /translation="MNQQLSWRAIVGYSLGDVANNFAFAMGALFLLSYYTDVAGVGAA
                     AAGTMLLLVRVFDAFADVFAGRVVDSVNTRWGKFRPFLLFGTAPLMIFSVLVFWVPTD
                     WSHSSKVVYAYLTYMGLGLCYSLVNIPYGSLATAMTQQPQSRARLGAARGIAASLTFV
                     CLAFLIGPSIKNSSPEEMVSVYHFWTIVLAIAGMVLYFICFKSTRENVVRIVAQPSLK
                     ISLQTLKRNRPLFMLCIGALCVLISTFAVSASSLFYVRYVLNDTGLFTVLVLVQNLVG
                     TVASAPLVPGMVARIGKKNTFLIGALLGTCGYLLFFWVSVWSLPVALVALAIASIGQG
                     VTMTVMWALEADTVEYGEYLTGVRIEGLTYSLFSFTRKCGQAIGGSIPAFILGLSGYI
                     ANQAQTPEVITGIRTSIALVPCGFMLLAFVIIWFYPLTDKKFKEIVVEIDNRKKVQQQ
                     L"
     gene            complement(1352..3163)
                     /gene="uidA"
                     /locus_tag="NBI74_003630"
     CDS             complement(1352..3163)
                     /gene="uidA"
                     /locus_tag="NBI74_003630"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HCD1933907.1"
                     /translation="MLRPVETPTREIKKLDGLWAFSLDRENCGIDQHWWESALQESRA
                     IAVPGSFNDQFADADIRNYVGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQ
                     EVMEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSY
                     FHDFFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDVSVELRD
                     ADQQVVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYLLRVGIRSVAV
                     KGEQFLINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYA
                     EEMLDWADEHGIVVIDETAAVGFNLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQAI
                     KELIARDKNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCD
                     AHTDTISDLFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVD
                     TLAGLHSMYTDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGN
                     KKGIFTRDRKPKSAAFLLQKRWTGMNFGEKPQQGGKQ"
     gene            complement(3553..4143)
                     /gene="uidR"
                     /locus_tag="NBI74_003631"
     CDS             complement(3553..4143)
                     /gene="uidR"
                     /locus_tag="NBI74_003631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator UidR"
                     /protein_id="HCD1933908.1"
                     /translation="MMDNMQTEAQPTRTRILNAAREIFSENGFHSASMKAICKSCAIS
                     PGTLYHHFISKEALIQAIILQDQERALARFREPIEGIHFVDYMVESIVSLTHEAFGQR
                     ALVVEIMAEGMRNPQVAAMLKNKHMTITEFVAQRMRDAQQKGEISPDINTAMTSRLLL
                     DLTYGVLADIEAEDLAREASFAQGLRAMIGGILTAS"
     gene            complement(4372..5139)
                     /gene="hdhA"
                     /locus_tag="NBI74_003632"
     CDS             complement(4372..5139)
                     /gene="hdhA"
                     /locus_tag="NBI74_003632"
                     /EC_number="1.1.1.159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-alpha-hydroxysteroid dehydrogenase"
                     /protein_id="HCD1933909.1"
                     /translation="MFNSDNLRLDGKCAIITGAGAGIGKEIAITFATAGASVVVSDIN
                     ADAANHVVDEIQQLGGQAFACRCDITSEQELSALADFAVSKLGKVDILVNNAGGGGPK
                     PFDMPMADFRRAYELNVFSFFHLSQLIAPEMEKNGGGVILTITSMAAENKNINMTSYA
                     SSKAAASHLVRNMAFDLGEKNIRVNGIAPGAILTNALKSVITPEIEQKMLQHTPIRRL
                     GQPQDIANAALFLCSPAASWVSGQILTVSGGGVQELN"
     gene            complement(5251..6279)
                     /gene="malI"
                     /locus_tag="NBI74_003633"
     CDS             complement(5251..6279)
                     /gene="malI"
                     /locus_tag="NBI74_003633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mal regulon transcriptional regulator MalI"
                     /protein_id="HCD1933910.1"
                     /translation="MATAKKITIHDVALAAGVSVSTVSLVLSGKGRISTATGERVNAA
                     IEELGFVRNRQASALRGGQSGVIGLIVRDLSAPFYAELTAGLTEALEAQGRMVFLLHG
                     GKDGEQLAQRFSLLLNQGVDGVVIAGAAGSSDDLRRIAEEKAIPVIFASRASYLDDVD
                     TVRPDNMQAAQLLTEHLIRNGHQRIAWLGGQSSSLTRAERVGGYCATLLKFGLPFHSD
                     WVLECTSSQKQAAEAITALLRHNPTISAVVCYNETIAMGAWFGLLKAGRQSGESGVDR
                     YFEQQVSLAAFTDATPTTLDDIPVIWASTPARELGTTLADRMMQKITHEETHSRNLII
                     PARLIAAK"
     gene            6454..8046
                     /gene="malX"
                     /locus_tag="NBI74_003634"
     CDS             6454..8046
                     /gene="malX"
                     /locus_tag="NBI74_003634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS maltose transporter subunit IICB"
                     /protein_id="HCD1933911.1"
                     /translation="MTAKTAPKVTLWEFFQQLGKTFMLPVALLSFCGIMLGIGSSLSS
                     HDVITLIPVLGNPVLQAIFTWMSKIGSFAFSFLPVMFCIAIPLGLARENKGVAAFAGF
                     VGYAVMNLAVNFWLTNKGILPTMDAAVLKANNIQSILGIQSIDTGILGAVIAGIIVWM
                     LHERFHNIRLPDALAFFGGTRFVPIISSLVMGLVGLVIPLVWPIFAMGISGLGHMINS
                     AGDFGPMLFGTGERLLLPFGLHHILVALIRFTDAGGTQEVCGQTVSGALTIFQAQLSC
                     PTTHGFSESATRFLSQGKMPAFLGGLPGAALAMYHCARPENRHKIKGLLISGLIACVV
                     GGTTEPLEFLFLFVAPVLYVIHALLTGLGFTVMSVLGVTIGNTDGNIIDFVVFGILHG
                     LSTKWYMVPVVAAIWFVVYYIIFRFAITRFNLKTPGRDSEVASSIEKAVAGAPGKSGY
                     NVPAILEALGGADNIVSLDNCITRLRLSVKDMSLVNVQALKDNRAIGVVQLNQHNLQV
                     VIGPQVQSVKDEMVGLMRTVQA"
     gene            8056..9228
                     /gene="malY"
                     /locus_tag="NBI74_003635"
     CDS             8056..9228
                     /gene="malY"
                     /locus_tag="NBI74_003635"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000459377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional maltose regulon transcriptional
                     repressor/cystathionine beta-lyase MalY"
                     /protein_id="HCD1933912.1"
                     /translation="MFDFSKVVDRHGTWCTQWDYVADRFGTADLLPFTISDMDFATAP
                     CIIEALNQRLIHGVFGYSRWKNDEFLAAIAHWFSTQHYTAIDPQSVVYGPSVIYMVSE
                     LIRQWSETGEGVVIHTPAYDAFYKAIEGNQRTVMPVALEKQADGWFCDMGKLEAVLAK
                     PECKIMLLCSPQNPTGKVWTCDELEIMADLCERHGVRVISDEIHMDMVWGEQPHIPWS
                     NVARGDWALLTSGSKSFNIPALTGAYGIIENSSSRDAYLSALKGRDGLSSPSVLALTA
                     HIAAYQQGAPWLDALRVYLKDNLTYIADKMNAAFPELNWQIPQSTYLAWLDLRPLNID
                     DNALQKALIEQEKIAIMPGYTYGEEGRGFVRLNAGCPRSKLEKGVAGLINAIRAVG"
     gene            9332..10333
                     /gene="add"
                     /locus_tag="NBI74_003636"
     CDS             9332..10333
                     /gene="add"
                     /locus_tag="NBI74_003636"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HCD1933913.1"
                     /translation="MIDTTLPLTDIHRHLDGNIRPQTILELGRQYNISLPAQSLETLT
                     PHVQVIANEPDLVSFLTKLDWGVKVLASLDACRRVAFENIEDAARNGLHYVELRFSPG
                     YMAMAHQLPVAGVVEAVIDGVREGCRTFGVQAKLIGIMSRTFGEAACQQELEAFLAHR
                     DQITALDLAGDELGFPGSLFLSHFNRARDAGWHITVHAGEAAGPESIWQAIRELGAER
                     IGHGVKAIEDRALMDFLAEQQIGIESCLTSNIQTSTVADLAAHPLKTFLEHGIRASIN
                     TDDPGVQGVDIIHEYTVAAPAAGLSREQIRQAQINGLEMAFLSAEEKRALREKVAAK"
     gene            complement(10369..11409)
                     /locus_tag="NBI74_003637"
     CDS             complement(10369..11409)
                     /locus_tag="NBI74_003637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310359.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HCD1933914.1"
                     /translation="MSDNIRVGVIGYGYASKTFHAPLIAGTPGLELAVISSSDETKVK
                     ADWPTVTVVSEPKHLFNDPNIDLIVIPTPNDTHFPLAKAALEAGKHVVVDKPFTVTLS
                     QARELDALAKTLGRVLSVFHNRRWDSDFLTLKGLLAEGVLGEVAYFESHFDRFRPQVR
                     DRWREQGGPGSGIWYDLAPHLLDQAITLFGLPVSMTVDLAQLRPGAQSTDYFHAILSY
                     PQRRVILHGTMLAAAESARYIVHGSRGSYVKYGLDPQEERLKNGERLPQEDWGYDMRD
                     GVLTRVEGEERVEETLLTVPGNYPAYYAAIRDALNGDGENPVPASQAIQVMELIELGI
                     ESAKHRATLCLA"
     gene            11652..11777
                     /gene="blr"
                     /locus_tag="NBI74_003638"
     CDS             11652..11777
                     /gene="blr"
                     /locus_tag="NBI74_003638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division septum protein Blr"
                     /protein_id="HCD1933915.1"
                     /translation="MNRLIELTGWIVLVVSVILLGVASHIDNYQPPEQSTTVQHK"
BASE COUNT         2913 a         3141 c         2909 g         3019 t
ORIGIN      
        1 attaattgct gctgcacttt tttacgatta tcaatttcaa caacgatttc tttgaatttt
       61 ttatccgtga gcggataaaa ccagataatg acgaatgcca gtagcataaa tccgcaaggt
      121 actaaggcaa ttgatgtgcg gatgcccgta ataacttccg gcgtttgcgc ctgattggcg
      181 atatatccgc ttaatcccaa aataaaggca ggaattgaac ctccgattgc ctgaccgcat
      241 ttacgggtaa atgagaatag tgaataggtg agcccttcaa ttcgcacgcc ggtcaggtat
      301 tcaccgtatt ctacggtatc agcttccagt gcccacatca cggtcatggt aacgccctgg
      361 ccaattgatg cgatggccaa cgcaaccaac gccaccggca gtgaccagac ggaaacccag
      421 aagaacagca gataaccgca ggttcccagc aaagcgccaa tcaggaaggt attcttttta
      481 ccgatcctcg cgaccatccc cggcaccagc ggtgccgatg ccacagtacc gaccaggttt
      541 tgcaccagta ccagcacagt gaacagcccg gtatcattta acacatagcg cacgtagaac
      601 aacgacgagg cgctgaccgc gaaggtcgaa atcagcacac acagcgcacc gatgcacaac
      661 ataaacagcg ggcgattccg tttcagggtt tgcagactga tcttcaatga cggctgcgcc
      721 acgatacgta ccacattctc acgcgtcgat ttgaagcaga tgaagtaaag caccattccg
      781 gcaatcgcca gcacaattgt ccagaaatga tataccgaca ccatctcttc cgggctggaa
      841 ttcttaatgc tcggtcctat cagaaatgcc aggcagacaa aggtcaatga agcggcaatc
      901 ccgcgagccg cgcccagacg ggcgcgggat tgtggttgtt gggtcatcgc ggtagcaagt
      961 gaaccataag gaatattcac caggctgtag caaagcccga gtcccatgta ggtcaaatat
     1021 gcatacacaa ctttgctgct atggctccag tcggtcggca cccagaatac cagcacgctg
     1081 aagatcatta acggcgcagt accgaagagt aaaaacgggc ggaattttcc ccatcgggta
     1141 ttcacactgt ccaccactcg tccggcaaag acatcggcga aggcatcgaa tacccgcacc
     1201 agtaacagca tggtgcccgc cgcagcggca ccgacgccag cgacgtcggt gtagtaactc
     1261 aacaggaaga gcgcccccat tgcgaaggcg aagttattgg cgacgtcacc gaggctgtag
     1321 ccgacgatgg cgcgccagga gagttgttga ttcattgttt gcctccctgc tgcggttttt
     1381 caccgaagtt catgccggtc cagcgttttt gcagcagaaa agccgccgac ttcggtttgc
     1441 ggtcgcgagt gaagatccct ttcttgttac cgccaacgcg caatatgcct tgcgaggtcg
     1501 caaaatcggc gaaattccat acctgctcac cgacgacagc gctgacgcga tcaaagacgc
     1561 ggtgatacat atccagccat gcacactgat actcttcact ccacatgtcg gtgtacattg
     1621 agtgcaggcc ggctaacgta tccacaccgt attcggtgat gataatcggc tgatgcagtt
     1681 tctcctgcca ggccagaagt tctttttcca gtaccttctc tgccgtttcc aaatcgccgc
     1741 tttggacata ccaaccgtaa taacggttca ggcacagcac atcaaagaga tcgctgatgg
     1801 tatcggtgtg agcgtcgcag aacattacat tgacacaggt gatcggacgc gtcgggtcga
     1861 gtttacgcgt tgcttccgcc agtggcgcaa aatattcccg tgcaccttgc ggacgggtat
     1921 ccggttcgtt ggcaatactc cacatcacca cgcttgggtg gtttttgtca cgcgcaatca
     1981 gctctttaat cgcctgtaag tgcgcctgct gggtttcccc gttgactgcc tcttcgctgt
     2041 acagttcttt cggcttgttg cccgcttcga aaccaatgcc taaagagagg ttaaagccga
     2101 cagctgcagt ttcatcaatc accacgattc catgttcatc tgcccagtcg agcatctctt
     2161 cagcgtaagg gtaatgcgag gtacggtagg agttggcccc aatccagtcc attagtgcgt
     2221 gatcgtgcac catcagcacg ttatcgaatc ctttgccgcg caaatccgca tcttcatgac
     2281 gaccaaagcc agtaaagtag aacggtttgt ggttgatcag gaactgttcg cccttcactg
     2341 ccactgaccg gataccgacg cgcagcaggt agatatcaca ctctgtctgg cttttggctg
     2401 tgacgcacag ttcatagaga taaccttcac ccggttgcca gaggtgcgga ttcactactt
     2461 gcaaagtccc gctggtgcct tgtccagttg caaccacctg ttgatccgca tcacgcagtt
     2521 caacgctgac atcaccattt gccaccacct gccagtcaac agatgcgtgg ttacagtctt
     2581 gcgcgacatg cgtcaccacg gtgatatcgt ccacccaggt gttcggcgtg gtgtagagca
     2641 ttacgctgcg atggatcccg gcgtagttaa agaaatcatg aaagtaagac tgctttttct
     2701 tgccgttttc gtcggtaatc accattcccg gcgggatagt ctgccagttc agttcgttgt
     2761 tcacacaaac agtgatacgt acacttttcc cggcaataac atacggtgtg acatcggctt
     2821 caaatggcgt atagccgccc tgatgctcca tcacttcctg attatttacc cacactttgc
     2881 cgtaatgagt gaccgcatcg aaacgcagta caatacgctg gcccgcccaa cctttcggta
     2941 taaaaacttc gcgctgatac cagacgttgc ccacataatt acgaatatct gcatcggcga
     3001 actgatcgtt aaaactgccc ggcacagcaa ttgcccggct ttcttgtaac gcgctttccc
     3061 accaatgctg atcaattcca cagttttcgc gatccagact gaatgcccac aggccgtcga
     3121 gttttttgat ttcacgggtt ggggtttcta caggacgtaa cataagggac tcctcattaa
     3181 gataaaaata ctggtcaacc tttaatctga ttagttaacc aattccgtgt tgcacacagt
     3241 tatctgtgag aaacccatgg aagagaaatg gaaataaaaa gaactgacac cgcgatcaaa
     3301 aaaccaaaaa ctgcaatacc cacagcgtga tattgatcgc attaatcttt aaaacagcgt
     3361 tgacagaagc cacaaaataa gcaatcgaac gttcattcga acatattggg aacgataaac
     3421 cctcatgata tattgctacg atgaatattt aggtggaatt ctccgatccc tgggaggatg
     3481 atttttgcac agagaaatta cgatatgaaa cgcgggctca gttgtcacca gcccgcggaa
     3541 agagagagag gatcaggatg cggttaatat accgccaatc atcgcgcgta atccctgagc
     3601 aaacgacgct tcacgcgcca gatcttccgc ttcgatatcg gccagtacac cgtaggtcag
     3661 atccagcagt aaacgtgaag tcattgccgt gttgatgtct gggcttatct cgcctttttg
     3721 ctgggcatca cgcatccgct gggcaacaaa ttccgtgatc gtcatatgct tatttttaag
     3781 catggcggcg acctgtgggt tacgcatccc ttccgccata atttcgacca ccagcgctcg
     3841 ttgcccaaag gcttcatggg tgagagagac aatagactcg accatatagt caacgaaatg
     3901 aatcccttca atcggttccc ggaaacgggc cagcgccctc tcctggtcct gtaagataat
     3961 cgcctgaatc aaggcttctt tggagatgaa atggtgatag agcgtcccgg gactaatggc
     4021 gcaggattta cagatggctt tcatcgaggc actgtgaaat ccattttctg aaaaaatctc
     4081 tctggcagca ttgaggatcc gggtccgtgt cggttgtgct tcagtctgca tgttatccat
     4141 cattaaacca ttatagttag catgaataac tagtttaccg cgttgtcagg tgagcgccta
     4201 tcattgctgt tgaatagtgc gaaggcacac tcaattcgta agctttttat ccagatattg
     4261 gttaaccaat agccgtgatt tggctgccag ttgtttagca gagacgttga cgagttgcac
     4321 acgctatccc ggtaaacaac acatacggct gtttaccggt tcgttagtgt attaattgag
     4381 ttcctgtacc ccaccaccgg agacggtgag aatttgtccg cttacccagc tggctgcagg
     4441 cgaacaaagg aacagcgccg cgttagcaat atcttgcggt tggcccagac gtctgattgg
     4501 tgtgtgttgc aacattttct gttcaatttc tggggtaata acggatttca gggcattggt
     4561 taatattgcc cccggcgcaa tgccattcac ccgaatattt ttttcaccca ggtcaaacgc
     4621 catatttctg accagatgac tggccgcagc tttagatgat gcataggagg tcatgtttat
     4681 atttttattt tctgccgcca tagaagtaat ggtcagaata acgccaccgc catttttttc
     4741 catttctggc gcaataagtt gtgacagatg gaaaaaagaa aatacattca gttcataagc
     4801 gcggcgaaaa tctgccattg gcatatcaaa cggtttaggc ccaccgccac cggcgttgtt
     4861 aaccaggata tcaactttac ccaacttact gacggcaaag tctgccagtg cagagagttc
     4921 ctgttcggaa gtaatatcgc agcggcaggc aaatgcctga ccacccagtt gttgaatttc
     4981 atctacaaca tgattagctg catcggcgtt aatatcactg accaccacag atgcgccagc
     5041 tgtcgcgaat gtaatggcga tttctttacc aatacctgca cccgcacctg taatgatggc
     5101 gcattttccg tcgagtctca ggttgtcaga attaaacaca taaacctcct gtgaacttca
     5161 gtaaaaataa aaggcaccgc ctgtgtgcca cattttttat cattaacctt gaggaaagga
     5221 cactaccagg tatagcagat aaagagagga ttatttcgcc gcaatgagcc gggcgggaat
     5281 aataagattg cgtgaatgcg tctcttcatg ggtgattttt tgcatcatgc gatccgcaag
     5341 tgtggtacca agttcccgcg ctggcgtgct ggcccagata acgggtatat catcaagtgt
     5401 ggtaggcgtt gcatcggtaa atgccgccag cgaaacctgt tgctcaaagt aacgatcgac
     5461 tccgctttcc ccgctttgcc ttcctgcttt cagcaaacca aaccatgccc ccatcgcaat
     5521 agtttcgtta tagcaaacca cggcgctgat ggtcgggtta tgacgtaaaa gcgccgtgat
     5581 agcttccgcg gcttgcttct ggctggaagt gcactccagc acccaatcgc tgtgaaacgg
     5641 caggccaaat tttagtagag tcgcacaata gccccccacc cgttctgccc gggttaatga
     5701 ggaactttgc cctcccagcc aggcgatacg ctgatgccca ttgcgaatga gatgctccgt
     5761 caacaactgt gcagcctgca tgttgtccgg gcgaaccgta tcaacgtcat caagataact
     5821 ggcacgggaa gcgaaaatca ccgggatagc tttttcttct gctatccgtc gcaggtcatc
     5881 gctgcttcct gcagccccgg caattaccac accatcgaca ccctgattca gtaatagtga
     5941 aaaccgctgt gccagctgct cgccgtcttt accgccgtga agcaaaaaaa ccatccgtcc
     6001 ctgcgcttcc agagcttccg tcaatccggc cgtcaattcg gcgtaaaacg gcgcagataa
     6061 atcacggacg atcaaaccaa tgacgccact ttgcccgccg cgcagcgccg acgcctgccg
     6121 attgcgcaca aatcccagct cttcaatggc ggcgttaacg cgttctcctg tggcggtaga
     6181 gattcgccct ttgccactaa gcaccagcga aacggtactt accgacacgc ccgcagccag
     6241 cgcaacatca tgaatggtta tttttttggc ggtagccatg cagatgacct actccctgat
     6301 tatgtgacag ataaaacgtt ttacctttta tttatcttat acccgctatt atcgttgcgt
     6361 aatgtgattt atgcctcact aaaatttgat aaaacgtttt atcttctcgt gcaatttact
     6421 gaatccagat tgttctctac gaggagtcgt tttatgacgg cgaaaacagc accgaaagtc
     6481 acgctgtggg agttcttcca gcagttaggc aaaaccttca tgttacccgt ggcattattg
     6541 tcgttctgcg gcattatgct cggcattggt agttctctta gcagccatga tgtcatcacc
     6601 ctgatcccgg tcctgggcaa ccccgtgttg caggctatct ttacctggat gagtaagatt
     6661 ggctcgtttg cttttagttt cctgccagtc atgttctgta tcgccatccc gctgggtctg
     6721 gcacgcgaaa acaaaggcgt agcggcattc gctggcttcg tcggttatgc ggtaatgaac
     6781 ctcgcggtaa acttctggtt gaccaataaa ggcattctgc caaccatgga cgccgccgtt
     6841 ctgaaagcca ataacatcca gagcattctt gggatccagt cgatcgacac cgggatcctc
     6901 ggtgcggtga tcgccggtat tatcgtctgg atgctgcatg agcgattcca taatatccgc
     6961 ctgccggatg cgctggcatt cttcggcggt acgcgcttcg taccaatcat ctcctcgctg
     7021 gtgatgggtc ttgtcggcct ggtgattcca ttagtctggc cgattttcgc catgggtatt
     7081 agcggcttag gccatatgat caacagcgcg ggtgatttcg gaccgatgct gtttggtacc
     7141 ggtgaacgtc tgctgttgcc gtttggtctg catcacattc tggtggcatt aattcgcttt
     7201 accgacgcag gcggcacgca ggaagtctgc ggtcaaaccg tcagcggtgc actgaccatc
     7261 ttccaggcgc aattgagttg cccgaccact cacggttttt ctgaaagcgc cacgcgtttc
     7321 ctttcgcaag gtaaaatgcc tgcgtttctc ggcggtctgc caggtgcagc gttagctatg
     7381 tatcactgtg cgcgcccgga aaatcgccat aaaattaaag gtctgctgat ttctggcctg
     7441 atcgcctgtg tcgttggcgg cacgaccgaa cctctggaat tcctgttcct gttcgtagcg
     7501 ccagttctgt atgtcatcca cgcgctgtta accggcctcg gcttcactgt catgtctgtg
     7561 ctcggcgtca ccatcggtaa taccgacggc aatatcatcg actttgtggt gttcggtatt
     7621 ttgcatggtc tgtcaaccaa gtggtacatg gtgccagtag tggcggcaat ctggtttgtc
     7681 gtttactaca tcatcttccg tttcgctatc acccgcttca atctcaaaac cccggggcgc
     7741 gatagtgaag ttgccagttc aatcgaaaaa gccgttgccg gtgcgccggg taaatcaggt
     7801 tacaacgttc cggcaatcct cgaagcctta ggcggtgccg acaatattgt cagtctcgat
     7861 aactgcatta cgcgtctgcg tttgtctgta aaagatatgt cgcttgttaa tgtgcaggca
     7921 ctgaaggaca atcgggcaat tggcgtagta caacttaatc aacataacct gcaggttgtt
     7981 atcgggccac aagttcagtc agtaaaagat gaaatggtcg gtctgatgcg tactgtccag
     8041 gcataaggat aagatatgtt cgatttttca aaggtcgtgg atcgtcatgg cacatggtgt
     8101 acgcagtggg attatgtcgc tgaccgtttc ggcactgctg acctgttacc gttcacgatt
     8161 tcagacatgg attttgccac tgccccctgc attatcgagg cgctgaatca gcgcctgata
     8221 cacggcgtgt ttggctacag ccgctggaaa aacgatgagt ttctcgcggc tattgcccac
     8281 tggttttcca cccagcatta caccgccatc gatcctcagt cggtggtgta tggcccttct
     8341 gttatctata tggtttcaga actgattcgt cagtggtctg aaacaggtga aggcgtggtg
     8401 atccacacac ccgcctatga cgcattttac aaggccattg aaggtaacca gcgcacagta
     8461 atgcccgttg ctttagagaa gcaggctgac ggctggtttt gcgatatggg caagctggaa
     8521 gccgtgctgg cgaaaccaga atgtaaaatt atgctcctgt gtagcccaca gaatcctacc
     8581 gggaaagtgt ggacgtgcga tgagctggag atcatggctg acctgtgcga gcgtcatggt
     8641 gtgcgggtta tttccgatga aatccatatg gatatggttt ggggcgagca gccgcatatt
     8701 ccctggagta atgtggcgcg cggagactgg gcgttgctaa cgtcgggatc aaaaagtttc
     8761 aatattcccg ccctgaccgg tgcttacggg attatagaaa atagcagtag ccgcgatgcc
     8821 tatttatcgg cactgaaagg acgtgatggg ctttcttccc cttcggtact ggcgttaact
     8881 gctcatatcg ccgcctatca gcaaggcgcg ccatggctgg atgccttacg cgtctatctg
     8941 aaagataacc tgacgtatat cgcagataaa atgaacgctg cgtttcctga actcaactgg
     9001 cagatcccgc aatccactta tctggcctgg cttgatctac gtccgttgaa tattgacgac
     9061 aacgcgttgc aaaaagcgct tatcgagcaa gaaaagatcg cgatcatgcc gggatatacc
     9121 tacggtgaag aaggtcgtgg ttttgtccga ctcaatgccg gctgcccacg ttcgaaactg
     9181 gaaaaaggtg tggctggatt aattaacgcc atccgcgctg ttggttaacc ccaattgcgc
     9241 aacgtaaaaa atcgttgcgc aatcgtggat ttttaccctg ctttgttttt ataatggtgc
     9301 gcacttttat atccagaaaa agagtgcgac catgattgat accaccctgc cattaactga
     9361 tatccatcgc caccttgatg gcaacattcg tccccagacc attcttgaac ttggccgcca
     9421 gtataatatc tcgcttcctg cacaatccct ggaaacactg actccccacg ttcaggtcat
     9481 tgccaacgaa cccgatctgg tgagctttct gactaaactt gactggggcg ttaaagttct
     9541 cgcctctctt gatgcctgcc gccgcgtggc atttgaaaac attgaagatg cagcccgtaa
     9601 cggcctgcac tatgtcgaac tgcgtttttc accaggctac atggcaatgg cacatcagct
     9661 gcctgtagcg ggtgttgtcg aagcggtgat cgatggcgta cgtgaaggtt gccgcacctt
     9721 tggtgtgcag gcgaagctta tcggtattat gagccggacc ttcggcgaag ccgcctgtca
     9781 gcaagagctg gaggcctttt tagcccaccg tgaccagatt accgcacttg atttagccgg
     9841 tgatgaactt ggtttcccgg gaagtctgtt cctttctcat ttcaaccgcg cgcgtgatgc
     9901 gggctggcat attaccgtcc atgcaggcga agctgccgga ccggaaagca tctggcaggc
     9961 gattcgtgaa ctgggggcgg agcgtattgg acatggcgta aaagccattg aagatcgggc
    10021 gctgatggat tttctcgccg agcaacaaat tggtattgaa tcctgtctga cctccaatat
    10081 tcagaccagc accgtggcgg atctggctgc acatccgctg aaaacgttcc ttgagcatgg
    10141 cattcgtgcc agcattaaca ctgacgatcc cggcgtgcag ggagtggata tcattcacga
    10201 atataccgtt gccgcgccag ctgctgggtt atcccgcgag caaatccgcc aggcacagat
    10261 taatggtctg gaaatggctt tcctcagcgc agaggaaaaa cgcgcactgc gagaaaaagt
    10321 cgccgcgaag taacaaaaat ggatggtgct aattgcacca tccccttttc acgcaaggca
    10381 caaagtcgcg cgatgtttgg cggattcgat gcccagctca atcaactcca ttacctggat
    10441 tgcctgactt gccggaaccg gattttcacc atcgccattt aacgcatcac gaatagccgc
    10501 atagtaagcc ggataattcc ccggcactgt caacagcgtt tcttcgacac gttcctcacc
    10561 ttccacgcgg gtaagtacgc catcacgcat atcgtagccc cagtcttcct gcggcagacg
    10621 ctcgccattt ttcagacgtt cttcctgtgg atcgaggcca tatttcacat aactgcctcg
    10681 ggatccatgc acgatatacc gtgctgactc agcggctgcc agcatggtgc cgtgtaaaat
    10741 cactcgccgc tgcggatagg acaagatagc gtggaaataa tcggtcgact gcgctccggg
    10801 ccgtaactgt gccaaatcta ccgtcatgct aaccggtaaa ccaaatagcg taatggcctg
    10861 atcaagaagg tgcggtgcta aatcgtacca gataccgctg cctgggccgc cctgttcacg
    10921 ccaacgatcg cgtacctgcg gacggaagcg gtcaaaatga gactcaaagt aagcaacttc
    10981 ccccagcacg ccttccgcga gcaaaccttt tagggtcaga aaatcgctat cccagcgacg
    11041 gttatggaat acagacaaca cacgccccag ggtttttgcc agcgcatcca gctctcgtgc
    11101 ttgtgacagt gtcacggtaa agggtttatc aacgaccaca tgtttacccg cctcaagcgc
    11161 cgctttagct aacggaaaat gggtatcgtt gggtgtagga atgacaatca ggtctatgtt
    11221 gggatcgtta aacagatgct tcggctcaga gacaaccgta accgttggcc agtcggcttt
    11281 tacttttgtt tcatcgctgc tggagattac cgccagttcc agcccgggcg tgcccgcaat
    11341 caggggcgca tggaaggttt tgctcgcata accataccca atcaccccaa cacggatgtt
    11401 gtcgctcatg tcattgcctc tcattgaagt atgatggcta tttgacacta tcctttaccc
    11461 tcgctcaaca gtttaataac ctgccagcaa taagggatgt tgtttaactt aagtcaaaaa
    11521 aatagcgatt tttccaacga caaaagctaa atatcgcaaa aacctcagta aaaatcttgc
    11581 tggagctatt attgctaagt aacatttacc ccctgaagtt aatggatcaa tcaacagaga
    11641 tgtgggctgt aatgaatcgt cttattgaat taacaggttg gatcgttctt gtcgtttcag
    11701 tcattcttct tggcgtggcg agtcacattg acaactatca gccacctgaa cagagtacta
    11761 cggtacaaca caagtaagct ctgcacttgt tgagcgacat gctgcccgtc cgggtgcatg
    11821 ttttcacttg tcggatatta aaccaggaat ttattatctt gttcgatgtt gttgatgatt
    11881 gtcagggata gtaaagcgcg ttactctttt cagagcagtt tttcatccct gaatatttgc
    11941 catcagcgca ttcttattat tgtaataaca caagcgttca ct
//
LOCUS       DAHHTG010000126        11753 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.127, whole genome shotgun sequence.
ACCESSION   DAHHTG010000126 DAHHTG010000000
VERSION     DAHHTG010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11753)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11753)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11753
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.127"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            63..665
                     /locus_tag="NBI74_003639"
     CDS             63..665
                     /locus_tag="NBI74_003639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebB family permuted papain-like enzyme"
                     /protein_id="HCD1933916.1"
                     /translation="MNINYPAEYEIGDIVFTCIGAALFGQISAASNCWNNHVGIIIGH
                     NGEDFLVAESRVPLSTITTLSRFIKRSANQRYAIKRLDAGLTEQQKQRIVEQVPSRLR
                     KLYHTGFKYESSRQFCSKFVFDTYKEALCIPVGEIETFGELLNSNPNAKLTFWKFWFL
                     GSIPWERKTVTPASLWHHPGLVLIHAVGVETPQPELTEAV"
     gene            complement(667..1188)
                     /gene="ruvC"
                     /locus_tag="NBI74_003640"
     CDS             complement(667..1188)
                     /gene="ruvC"
                     /locus_tag="NBI74_003640"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005068993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HCD1933917.1"
                     /translation="MAIILGIDPGSRVTGYGVIRQVGRQLSYLGSGCIRTKVDDLPSR
                     LKLIYAGVTEIITQFQPDYFAIEQVFMAKNADSALKLGQARGVAIVAAVNQELPVFEY
                     AARQVKQTVVGIGSAEKSQVQHMVRTLLKLPANPQADAADALAIAITHCHVSQNAMQM
                     SESRLNLARGRLR"
     gene            complement(1223..1963)
                     /locus_tag="NBI74_003641"
     CDS             complement(1223..1963)
                     /locus_tag="NBI74_003641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCD1933918.1"
                     /translation="MAGHSKWANTRHRKAAQDAKRGKIFTKIIRELVTAAKLGGGDPD
                     ANPRLRAAIDKALSNNMTRDTLNRAIARGVGGDDDANMETIIYEGYGPGGTAIMIECL
                     SDNRNRTVAEVRHAFSKCGGNLGTDGSVAYLFSKKGVISFEKGDEDTIMEAALEAGAE
                     DVVTYDDGAIDVYTAWEEMGKVRDALEAAGLKADSAEVSMIPSTKADMDAETAPKLMR
                     LIDMLEDCDDVQEVYHNGEISDEVAATL"
     gene            complement(1992..2444)
                     /gene="nudB"
                     /locus_tag="NBI74_003642"
     CDS             complement(1992..2444)
                     /gene="nudB"
                     /locus_tag="NBI74_003642"
                     /EC_number="3.6.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate diphosphatase"
                     /protein_id="HCD1933919.1"
                     /translation="MTDMAYKRPVSILVVIYAQDTKRVLMLQRRDDPDFWQSVTGSVE
                     EGETAPQAAMREVKEEVTIDVVAEQLTLIDCQRTVEFEIFSHLRHRYAPGVTRNTESW
                     FCLALPHERQIVFTEHLAYKWLDAPAAAALTKSWSNRQAIEQFVINAA"
     gene            complement(2437..4209)
                     /gene="aspS"
                     /locus_tag="NBI74_003643"
     CDS             complement(2437..4209)
                     /gene="aspS"
                     /locus_tag="NBI74_003643"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HCD1933920.1"
                     /translation="MRTEYCGQLRLSHVGQQVTLCGWVNRRRDLGSLIFIDMRDREGI
                     VQVFFDPDRADALKLASELRNEFCIQVTGTVRARDEKNINRDMATGEIEVLASSLTII
                     NRADVLPLDSNHVNTEEARLKYRYLDLRRPEMAQRLKTRAKITSLVRRFMDDHGFLDI
                     ETPMLTKATPEGARDYLVPSRVHKGKFYALPQSPQLFKQLLMMSGFDRYYQIVKCFRD
                     EDLRADRQPEFTQIDVETSFMTAPQVREVMEALVRHLWLEVKGVDLGDFPVMTFAEAE
                     RRYGSDKPDLRNPMELTDVADLLKSVEFAVFAGPANDPKGRVAALRVPGGASLTRKQI
                     DEYGNFVKIYGAKGLAYIKVNERAKGLEGINSPVAKFLNAEIIEAILERTGAQDGDMI
                     FFGADNKKIVADAMGSLRLKVGKDLGLTDESKWAPLWVIDFPMFEDDGEGGLTAMHHP
                     FTSPKDMTAAELKAAPENAVANAYDMVINGYEVGGGSVRIHNGDMQQTVFGILGINEE
                     EQREKFGFLLDALKYGTPPHAGLAFGLDRLTMLLTGTDNIRDVIAFPKTTAAACLMTE
                     APSFANPTALAELSIQVVKKAENN"
     gene            4519..5085
                     /locus_tag="NBI74_003644"
     CDS             4519..5085
                     /locus_tag="NBI74_003644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HCD1933921.1"
                     /translation="MLELNAKTTALVVIDLQEGILPFAGGPHTADEVVNRAGKLAAKF
                     RASGQPVFLVRVGWSADYAEALKQPVDAPSPAKVLPENWWQHPVALGATDSDIEIIKR
                     QWGAFYGTDLELQLRRRGIDTIVLCGISTNIGVESTARNAWELGFNLVIAEDACSAAS
                     AEQHNNSINHIYPRIARVRSVEEILHAL"
     gene            5082..5900
                     /locus_tag="NBI74_003645"
     CDS             5082..5900
                     /locus_tag="NBI74_003645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HCD1933922.1"
                     /translation="MIYIGLPQWSHPKWVRLGITSLEEYARHFNCVEGNTTLYALPKP
                     EVVLRWREQTTDDFRFCFKFPATISHQAALRHCDDLVTEFLTRMSPLAPRIGQYWLQL
                     PATFGPRELPALWHFLDSLPGEFNYGVEVRHPQFFAKGEEEQTLNRGLHQRGVNRVIL
                     DSRPVHAARPHSEAIRYAQRKKPKVPVHAVLTAKNPLIRFIGSDDMTQNRELFQVWLQ
                     KLAQWHQTTTPYLFLHTPDIAQAPELVHTLWEDLRKTLPEIGAVPAIPQQSSLF"
     gene            5953..6348
                     /locus_tag="NBI74_003646"
     CDS             5953..6348
                     /locus_tag="NBI74_003646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HCD1933923.1"
                     /translation="MVSALYAVLSALLLMKFSFDVVRLRMQYRVAYGDGGFSELQSAI
                     RIHGNAVEYIPIAIVLMLFMEMNGAETWMVHICGIVLLAGRLMHYYGFHHRLFRWRRS
                     GMSATWCALLLMVLANLWYMPWELVFSLR"
     gene            6389..7132
                     /gene="cmoA"
                     /locus_tag="NBI74_003647"
     CDS             6389..7132
                     /gene="cmoA"
                     /locus_tag="NBI74_003647"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy-S-adenosyl-L-methionine synthase CmoA"
                     /protein_id="HCD1933924.1"
                     /translation="MSHRDTLFSAPIARLGDWTFDERVAEVFPDMIQRSVPGYSNIIS
                     MIGMLAERFVQPGTQVYDLGCSLGAATLSVRRNIHHDNCKIIAIDNSPAMIERCRRHI
                     DAYKAPTPVDVIEGDIRDIAIENASMVVLNFTLQFLEPSERQALLDKIYQGLNPGGAL
                     VLSEKFSFEDAKVGELLFNMHHDFKRANGYSELEISQKRSMLENVMLTDSVETHKARL
                     HKAGFEHSELWFQCFNFGSLVALKAEDAA"
     gene            7129..8100
                     /gene="cmoB"
                     /locus_tag="NBI74_003648"
     CDS             7129..8100
                     /gene="cmoB"
                     /locus_tag="NBI74_003648"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-methoxyuridine(34)/uridine 5-oxyacetic
                     acid(34) synthase CmoB"
                     /protein_id="HCD1933925.1"
                     /translation="MIDFGNFYSLIAKNHLSHWLETLPTQIANWQREQQHGLFKQWSN
                     AVEFLPEIKPYRLDLLHSVTAESEEPLSAGQIKRIETLMRNLMPWRKGPFSLYGVNID
                     TEWRSDWKWDRVLPHLSDLTGRTILDVGCGSGYHMWRMIGAGAHLAVGIDPTQLFLCQ
                     FEAVRKLLGNDQRAHLLPLGIEQLPALKAFDTVFSMGVLYHRRSPLEHLWQLKDQLVN
                     EGELVLETLVIDGDENTVLVPGDRYAQMRNVYFIPSALALKNWLKKCGFVDIRIADVS
                     VTTTEEQRRTEWMVTESLADFLDPHDPGKTVEGYPAPKRAVLIARKP"
     gene            complement(8136..10565)
                     /gene="torZ"
                     /locus_tag="NBI74_003649"
     CDS             complement(8136..10565)
                     /gene="torZ"
                     /locus_tag="NBI74_003649"
                     /EC_number="1.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310609.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine N-oxide reductase TorZ"
                     /protein_id="HCD1933926.1"
                     /translation="MTLTRREFIKHSGIAAGALVVTSAAPLPAWAEEKGGKILTAGRW
                     GAMNVEVKDGKIVSSTGALAKTIPNSLQSTAADQVHTTARIQHPMVRKSYLDNPLQPA
                     KGRGEDTYVQVSWEQALKLIHEQHDHIRKANGPSAIFAGSYGWRSSGVLHKAQTLLQR
                     YMNLAGGYSGHSGDYSTGAAQVIMPHVVGSVEVYEQQTSWPLILENSQVVVLWGMNPL
                     NTLKIAWSSTDEQGLEYFHQLKKSGKPVIAIDPIRSETIEFFGDNATWIAPNMGTDVA
                     LMLGIAHTLMTQGKHDKVFLEKYTTGYPQFEEYLTGKNDNTPKSAAWAAEITGVPEAQ
                     IVKLAELMAANRTMLMAGWGIQRQQYGEQKHWMLVTLAAMLGQIGTPGGGFGFSYHYS
                     NGGNPTRVGGVLPEMSAAIAGQASEAADDGGMTAIPVARIVDALENPGGKYQHNGKEQ
                     TYPNIKMIWWAGGGNFTHHQDTNRLIKAWQKPEMIVVSECYWTAAAKHADIVLPITTS
                     FERNDLTMTGDYSNQHIVPMKQAVAPQFEARNDFDVFADLAELLKPGGKEIYTEGKDE
                     MAWLKFFYDAAQKGARAQRVTMPMFNAFWQQNKLIEMRRSEKNEQYIRYGDFRADPVK
                     NALGTPSGKIEIYSRTLEKFGYKDCPAHPTWLAPDEWKGTADEKQLQLLTAHPAHRLH
                     SQLNYAELRKKYAVADREPITIHTEDAARFGIANGDLVRVWNKRGQILTGAVVTDGIK
                     KGVVCVHEGAWPDLENGLCKNGSANVLTADIPSSQLANACAGNSALVYIEKYTGNALK
                     LTAFDQPAIQA"
     gene            complement(10590..11690)
                     /locus_tag="NBI74_003650"
     CDS             complement(10590..11690)
                     /locus_tag="NBI74_003650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NapC/NirT family cytochrome c"
                     /protein_id="HCD1933927.1"
                     /translation="MRGKKRIGLLFLLIAVVVGGGGLLLAQKALHKTSDTAFCLSCHS
                     MSKPFEEYQGTVHFSNQKGIRAECADCHIPKSGMDYLFAKLKASKDIYHEFVSGKIDS
                     DDKFEAHRQEMAETVWKELKATDSATCRSCHSFDAMDISSQSESAQKMHNKAQKDGET
                     CIDCHKGIAHFPPEIKMDDNAAHELESQAATSVTNGAHIYPFKTSRIGDLATVTPGTD
                     LTVVDVSGKQPIVRLQGYQMQGSENTLYLAAGQRLALATLSEEGIKALTVNGEWQADE
                     YGNQWRQASLQGALTDSALADRKPLWQYAEKLDDTYCAGCHAPIAADHYTVNAWPSIA
                     KGMGARTSMSENELDILTRYFQYNAKDITEKQ"
BASE COUNT         2691 a         3102 c         2870 g         3090 t
ORIGIN      
        1 ctgaaaaaat aaatttttta tgctattacg tatattcata tctatttcaa tggaatgaca
       61 acgtgaatat taattatcct gcggaatatg aaattggtga tatcgtcttt acatgtatag
      121 gtgctgcctt atttggtcaa atatcagctg catcaaattg ctggaataat cacgtcggga
      181 tcattatcgg tcataacggt gaagactttc tggttgcaga aagccgtgtt cccctctcaa
      241 ccatcactac gctatcccgt tttattaaac gctctgctaa tcaacgctat gctataaagc
      301 gattagacgc cggactaaca gaacaacaaa aacaacgaat tgttgaacag gttccttccc
      361 ggctacgcaa actttaccac accggtttta aatacgaatc ttcgcgacag ttctgttcaa
      421 aatttgtttt tgatacttat aaagaggcgc tatgtattcc ggtgggtgaa atagagacgt
      481 ttggagaatt attaaatagc aatccgaatg caaaactcac tttctggaaa ttctggttct
      541 taggttctat tccgtgggag cgtaaaaccg tcacgccagc cagtttgtgg catcatccgg
      601 gtttggtgtt gattcacgcg gtgggagttg aaacgcctca gcctgaactg accgaggcgg
      661 tataacttaa cgcagtcgcc ctctcgccag gttcagtcgc gattcactca tttgcatcgc
      721 attctgacta acgtggcagt gggtgatggc aatcgccagc gcatcggcgg catccgcctg
      781 tggattagcg ggcagtttca gcaaggtgcg gaccatatgc tgcacctggc ttttttcggc
      841 actaccaata cctaccactg tttgctttac ctgacgtgcc gcatattcaa ataccggcaa
      901 ttcctgattc accgccgcca caatcgccac gccgcgcgcc tgccccagtt tcagggctga
      961 gtcagcgttc ttcgccataa agacttgttc aatggcgaaa taatcaggct ggaattgggt
     1021 gatgatttcc gtcacgcccg catagatgag cttcagacga gacggtaaat catccacttt
     1081 ggtgcgtatg catccgctac ccaggtagga cagttgcctg cctacctggc ggatgacgcc
     1141 atagccggtc acgcgcgaac ccgggtcaat accgagaata atagccatca cgcgtctccg
     1201 ttttgctgtt tagcaggcct catcagagag tcgctgcaac ctcatcagag atttcaccgt
     1261 tatggtaaac ttcctgcacg tcgtcgcaat cttccagcat atcgatcaga cgcatcagtt
     1321 tcggtgcggt ttctgcatcc atatcagctt tggtggacgg gatcatggaa acttccgcgc
     1381 tgtctgcttt cagacctgcc gcttccagag cgtcgcgtac tttgcccatt tcttcccatg
     1441 cagtgtagac atcaatcgcg ccgtcatcat aggtcacaac gtcttcagca ccggcttcca
     1501 gggctgcttc catgatggtg tcttcatcgc ctttctcgaa ggagatcacg ccttttttgc
     1561 tgaacagata agctacggaa ccatcagtac cgaggttacc gccacatttg ctgaatgcat
     1621 gacgcacttc agcaacggta cggttgcggt tgtcagacag acattcaatc atgattgccg
     1681 tgccgccagg accgtaacct tcgtagatga tggtttccat gtttgcatca tcatcaccgc
     1741 ccacacctcg tgcaattgcg cggttcagag tgtcacgggt catgttgtta gacagtgctt
     1801 tatcaattgc tgcacgcaga cgcggattag cgtccggatc accaccgccc agcttagccg
     1861 cggttaccag ctcacgaatg attttagtga agattttacc gcgcttagca tcctgcgcag
     1921 ctttacgatg tctggtgttg gcccatttac tatgacctgc cataaaaata tctccagata
     1981 gccctgcctg ttcaggcagc gttaattaca aactgttcaa tcgcctgccg gttgctccag
     2041 gacttagtga gcgccgccgc agcaggcgca tcaagccact tgtaagccag atgttcagtg
     2101 aaaacgatct ggcgctcgtg cgggagcgca agacagaacc atgattccgt attacgcgtc
     2161 acgcccggcg catagcgatg acgtaaatgt gaaaaaattt caaactctac cgtgcgctga
     2221 cagtcaatta aggtcagttg ttcagcgaca acatcaatgg tgacctcttc ctttacttcg
     2281 cgcatggcag cttgcggcgc ggtttcaccc tcttccacgc tgccggttac cgactgccag
     2341 aaatcgggat cgtcacgccg ctgcaacatc agcacccgtt tcgtatcttg tgcgtagatg
     2401 accactaaga tcgaaacggg acgcttataa gccatatcag ttattctcag ccttcttcac
     2461 aacctgaatg ctcagctcag ccagtgcagt cgggttagca aagctcggtg cttcagtcat
     2521 cagacacgcc gccgccgtgg ttttcgggaa ggcgataacg tcacggatat tgtcggtgcc
     2581 ggtcagcagc atggtcagac ggtcaagacc gaatgccaga cctgcgtgcg gcggagtacc
     2641 gtatttcagg gcgtcgagca ggaagccgaa tttctcgcgc tgttcctctt cgttgatacc
     2701 cagaatacca aacaccgtct gctgcatatc accattatgg atacgcacag aaccaccgcc
     2761 cacttcgtaa ccattgatga ccatatcgta agcgttagcc accgcatttt ccggtgcagc
     2821 tttcagttct gctgccgtca tgtctttcgg tgaggtgaac ggatggtgca ttgctgtcag
     2881 gccgccttca ccgtcgtctt caaacatcgg gaagtcgata acccacagcg gtgcccattt
     2941 gctttcgtcg gtcagaccaa ggtctttacc cactttcagg cgcagtgagc ccatcgcgtc
     3001 ggcaacaatt ttcttgttgt cggcaccgaa gaaaatcata tcgccatctt gcgcgccagt
     3061 acgctccagg atggcttcga tgatttctgc attaaggaac ttcgctaccg ggctgttgat
     3121 accttccaga cctttcgcgc gttcgttaac tttgatgtaa gccagacctt tcgcgccgta
     3181 gattttaacg aagttaccgt attcgtcgat ctgcttacgg gtcaacgatg cgccgcccgg
     3241 aacacgcaga gcggcaacac ggcctttcgg atcgttcgcc ggacctgcaa atactgcaaa
     3301 ctcaacagat ttcagcagat cggcaacgtc ggtcagttcc atcgggttac gtagatctgg
     3361 tttatcagaa ccataacggc gttctgcttc tgcaaaggtc attaccggga aatcgcccag
     3421 atccacgccc ttcacttcca gccacagatg acgcaccagc gcttccatca cttcacgcac
     3481 ttgcggcgcg gtcatgaaag aagtttccac atcgatctga gtaaattcag gctgacggtc
     3541 agcacgcagg tcttcgtcac ggaagcattt aacgatctga tagtagcggt caaagccgga
     3601 catcatcagc agctgtttga acaactgtgg ggattgcggc agtgcgtaga atttaccttt
     3661 gtgcacacga gaaggcacca ggtagtcacg cgcgccttcc ggcgtggctt tggtcagcat
     3721 cggagtttcg atgtcgagga agccgtggtc atccataaaa cggcgcacca ggctggtgat
     3781 tttagcgcgg gttttcaggc gctgagccat ttccgggcga cgcaggtcga ggtagcggta
     3841 tttcagacgc gcttcttcgg tgttgacgtg gttagagtca agcggcagaa catctgcacg
     3901 gttgatgata gtcagcgagg acgccagtac ttcgatttcg ccagtcgcca tatcgcggtt
     3961 aatatttttt tcgtcacgcg cacgtacggt gcccgtgacc tgaatgcaga actcattacg
     4021 cagttcagag gccagcttta acgcgtccgc acgatccgga tcgaaaaata cctgcacgat
     4081 accttcgcgg tcgcgcatat cgatgaagat caggctacca agatcacgac gacggttgac
     4141 ccaaccacac agagtcacct gctgccccac gtgggacaaa cggagctgtc cacaatattc
     4201 tgtacgcatg agatatccct taacttagct gccggcggat gccccctgct gcgcaggtga
     4261 ccaagtcgca gcgttagctg tatgtcacaa ctgaatgaaa aaaggcggct attatactgg
     4321 aaattctgcc gcaccgtaag agcctggccc gcgctggaac gcctcgttac cactttatat
     4381 cgggcctgaa atcagactct acgccagttt gctataaagg tgttgcccga actcataaaa
     4441 attaacaaaa tttgtcgttc cgccatcggc tcatcgtatt aaggtgagag gcacgatttt
     4501 gttttatcag gagtcatcat gcttgaactt aatgctaaaa ccaccgcgct ggtggtgatt
     4561 gatttacaag aaggcatctt gccttttgcc ggtggtccac atactgccga tgaggtggtt
     4621 aatcgcgccg ggaagctggc ggcgaaattt cgcgcgagcg gtcagcccgt gtttctggtg
     4681 cgtgttggct ggtctgccga ttatgctgaa gcattaaaac agccggttga tgccccttcc
     4741 cccgctaaag tgttgcccga aaattggtgg caacatcctg tagcattagg tgcaaccgac
     4801 agcgatatcg aaatcatcaa acgtcaatgg ggtgcgtttt acggtacgga tctggaattg
     4861 cagttgcgcc gccggggtat cgatacaata gtgttatgtg ggatctcgac caatatcggt
     4921 gttgaatcca ccgcccgcaa tgcctgggaa ctcggtttta atctggtgat tgccgaagat
     4981 gcctgtagcg ccgctagcgc cgagcagcac aataacagca ttaatcatat ctacccgcgc
     5041 atcgcccgtg tgcgtagcgt tgaagagatc ctccacgcgt tatgatttac atcggtttgc
     5101 cacaatggtc gcatcctaaa tgggtgcggt tggggatcac cagccttgaa gagtatgccc
     5161 gccactttaa ctgtgtggag ggtaacacca cgctttatgc cctgccgaaa cccgaggttg
     5221 tcctgcgctg gcgtgagcag accacagatg acttccgctt ctgttttaag tttccggcga
     5281 ctatttcgca tcaggcagca ttacggcatt gcgatgattt agtgactgaa tttttgaccc
     5341 gcatgtcacc gttggctccg cgcattggac aatactggct gcaactgcct gccacattcg
     5401 gcccacggga gctgcctgcg ctttggcatt ttctcgattc tcttcccggc gaatttaatt
     5461 atggtgtgga agtccgccat ccacagtttt tcgccaaagg ggaagaggaa caaacgctta
     5521 atcgcggttt acatcagcgc ggcgttaatc gggtgatttt agacagccgc ccggttcatg
     5581 cagcacgtcc acacagtgaa gctattcgct acgctcaacg aaaaaaacct aaagttccgg
     5641 tacatgctgt actgacggcg aaaaatccac tgatccgttt tatcggtagt gatgatatga
     5701 cgcaaaaccg ggaattattt caggtctggt tacaaaaatt agcgcagtgg catcagacca
     5761 ctacgcctta tcttttttta catacgccag atattgccca ggccccggaa ctggtacata
     5821 ccctgtggga agacttacgt aaaacgcttc cagagatcgg agcagttccg gctattccac
     5881 agcaatcttc tcttttctga atttgccacc tatcatagac aggtgccatc ggccatttta
     5941 aagggagttt gtatggtaag cgcgctgtat gccgttttaa gtgcgttgtt attaatgaag
     6001 ttctcttttg atgtcgttcg cctgcgaatg cagtaccgcg ttgcctatgg cgacggcggt
     6061 tttagcgaac tgcaaagcgc tattcgcatt catggtaacg cggtggaata tattcccatc
     6121 gcgattgtgt tgatgctgtt tatggaaatg aatggcgcag aaacctggat ggtgcatatt
     6181 tgcggcatcg ttttgcttgc tggtcgtctg atgcattatt acggttttca tcaccgtctg
     6241 ttccgctggc gacgttctgg catgagcgcc acctggtgtg cgctgttgct gatggtgctg
     6301 gcgaatcttt ggtatatgcc ctgggagttg gttttctccc tgcgttagcg cacaatacgc
     6361 cactttcttt ttcccggatt tttacgttat gtctcaccgc gacacgctat tttctgcccc
     6421 tatcgccaga ctgggcgact ggacctttga tgaacgggta gctgaagtct tcccggatat
     6481 gatccagcgt tccgttcccg gctattccaa tattatttcc atgattggta tgttagccga
     6541 gcgcttcgtt caacctggta cgcaggttta cgatctgggt tgttctctgg gcgcggcgac
     6601 actctcggtg cgtcgcaaca ttcatcatga taattgcaaa attattgcca tcgataactc
     6661 cccggcgatg attgaacgct gccgtcgtca tattgacgcc tataaagctc ctacgccagt
     6721 agacgttatt gaaggtgata ttcgcgatat cgccattgaa aacgcatcga tggtggtgct
     6781 gaattttacc ctgcaattcc tggaaccttc cgagcgccag gcgttactgg ataaaattta
     6841 tcaaggactg aacccgggcg gcgcgctggt gctttcggaa aaattcagtt tcgaagatgc
     6901 caaagttggt gaactgctgt tcaacatgca ccacgacttt aaacgtgcca acggttacag
     6961 cgaactggag atcagccaga aacgcagcat gctggaaaac gtgatgctga ccgattccgt
     7021 ggaaacccat aaagcacgcc tgcataaagc cggttttgag catagcgagc tgtggttcca
     7081 gtgctttaac tttggttcac tggtggcatt aaaagcagag gacgctgcat gatcgacttt
     7141 ggtaactttt attctctgat tgccaaaaat catctttcac actggctcga aacgctgccc
     7201 acgcagattg ctaactggca gcgcgagcag cagcacgggc tgtttaagca gtggtccaac
     7261 gcggtggaat ttctgcctga aattaaaccg tatcgtctgg atttattgca tagcgtaacc
     7321 gccgaaagcg aagagccact gagcgccggg caaattaagc gcattgaaac gctgatgcgc
     7381 aacctgatgc cgtggcgcaa agggccgttc tcactgtatg gcgtcaacat cgataccgaa
     7441 tggcgttccg actggaaatg ggatcgcgtt ctgccccatc tttctgattt aaccgggcgc
     7501 accattcttg atgtcggctg cggcagcggt tatcacatgt ggcgcatgat tggcgctggc
     7561 gcgcacctcg cggtggggat cgaccctaca caactgtttc tgtgccagtt tgaagcggtg
     7621 cgtaaactac tgggtaacga tcagcgcgca catttgttac cgttaggtat tgaacaactt
     7681 ccggcactga aagcctttga taccgtcttt tcgatgggcg tgctttatca tcgtcgttca
     7741 ccgctggagc atctctggca gttaaaagac caactggtga atgaaggcga actggtgctg
     7801 gaaacgctgg ttattgatgg cgatgagaac acggtgctgg tgccgggcga tcgttacgca
     7861 caaatgcgta atgtctattt cattccttcc gcgctggcgc tgaaaaactg gctgaagaag
     7921 tgtggttttg ttgatattcg cattgcggat gtgagcgtta ccaccacaga agagcagcga
     7981 cgcaccgaat ggatggtcac cgagtctctg gccgattttc tcgacccgca tgatccgggt
     8041 aaaacggtgg aaggttatcc tgcgcctaaa cgcgcggtgc tgattgcgcg caagccgtaa
     8101 aaatttagcg cccgtttccg ggcgcttttt acgtcttatg cctgaatagc tggctgatca
     8161 aacgccgtta acttcagcgc attgcccgta tatttttcga tatacaccag cgcagagtta
     8221 ccggcacagg cattcgccag ctgcgagctg gggatatccg ccgttaacac gttcgcacta
     8281 ccgtttttac acaagccatt ttccagatct ggccatgcac cttcatgcac gcataccacg
     8341 ccttttttga tcccgtcagt caccaccgcg cctgtcagaa tctgaccacg tttgttccag
     8401 acacgcacca gatcgccatt cgcaatacca aagcgagcag catcttcggt gtgaatagtg
     8461 attggttcac gatcggcaac cgcatatttt ttacgcagtt ccgcatagtt aagctgactg
     8521 tgtaaacggt gtgccggatg tgcggtcaga agctgcaact gcttctcgtc ggcggtaccc
     8581 ttccactcat caggcgcaag ccaggttggg tgtgccgggc aatccttata gccaaatttt
     8641 tccagcgttc tggagtaaat ctcaattttg ccgcttggcg tacccagcgc atttttcacc
     8701 ggatcggcgc ggaaatcacc ataacgaatg tactgttcgt tcttctcgct gcggcgcatt
     8761 tcgatcagtt tattttgctg ccagaaggca ttaaacatcg gcatcgtcac gcgttgcgca
     8821 cgggcacctt tctgagcggc atcatagaaa aatttcagcc acgccatttc atctttacct
     8881 tcggtataga tctcttttcc gccaggtttg agtaattcag caagatcggc aaacacgtca
     8941 aaatcgttgc gcgcttcaaa ttgcggagcg acagcctgct tcatcggcac aatatgctgg
     9001 ttgctgtaat caccggtcat cgtcaggtca ttgcgctcaa acgatgtggt gatcggtaat
     9061 acgatatctg cgtgtttggc tgctgcggtc cagtagcatt cagaaacgac gatcatctcc
     9121 ggtttctgcc atgctttaat cagacgatta gtatcctggt gatgggtaaa gttaccgcca
     9181 cccgcccacc agatcatttt gatattcgga tacgtttgtt ctttaccgtt atgctgatat
     9241 ttgccgccag gattttccag cgcatcgacg atacgggcaa caggaatcgc cgtcattccg
     9301 ccatcatcag cggcctcgct ggcctgtccg gcaatcgccg cagacatttc tggtagcacg
     9361 ccgccaacac gcgtcgggtt gccaccgttg gagtaatgat aagagaaacc aaacccgccg
     9421 cccggtgtac caatttgccc taacatcgcc gccagcgtca ccagcatcca gtgtttctgt
     9481 tcaccgtatt gctggcgctg aattccccag cctgccatca gcattgtgcg atttgccgcc
     9541 atcagttctg caagtttcac aatctgcgct tccggcacgc cagtaatttc cgccgcccag
     9601 gccgcactct tcggcgtatt atcgttcttg cctgtcagat actcttcaaa ctgcggatac
     9661 ccggtagtgt atttttcaag gaacacttta tcgtgtttgc cttgtgtcat cagggtatgc
     9721 gcaatcccta acatcagtgc cacgtcggtg cccatattcg gtgcgatcca ggtggcatta
     9781 tcgccaaaga attcgatggt ttcggagcgg ataggatcaa tggcaatcac tggtttgcca
     9841 gattttttca gctgatggaa gtattccagc ccttgttcat cggtactgct ccaggcaatt
     9901 tttaaggtat tcagcgggtt catgccccac aacaccacca cctgactgtt ttccagaatc
     9961 agcggccagg aagtctgctg ttcatagacc tccacagaac cgaccacatg cggcatgatc
    10021 acctgtgccg ctccggttga ataatcgccg ctatgcccgg aataaccgcc cgccaggttc
    10081 atataacgtt gcagtaaggt ttgcgcttta tgcaacacgc cagaagaacg ccagccgtaa
    10141 gatccggcaa aaatggccga tggtccgtta gctttacgaa tatgatcgtg ttgctcatga
    10201 atcagtttta atgcttgttc ccagctcacc tgtacatagg tatcttcgcc acgacctttc
    10261 gccggttgca gtggattatc gagatagctt tttctcacca tcggatgctg aatacgcgcc
    10321 gtggtgtgta cctgatccgc cgccgtagac tgtaaggagt tcggtatggt cttcgccagc
    10381 gcgcctgtcg aagaaacaat cttgccgtcc ttcacttcta cgttcatcgc tccccaacgt
    10441 cccgcggtga gaattttgcc gcccttctct tctgcccatg caggtaacgg tgcagccgat
    10501 gtcaccacca gcgccccagc ggcaataccg ctatgtttaa taaattcgcg tcttgttaat
    10561 gtcataactt cctccctgat caacgaggat cactgtttct cggtaatatc tttggcgttg
    10621 tactggaagt accgcgtcaa aatgtccagt tcgttttcgc tcatgctggt tcgcgcaccc
    10681 attcctttgg caatggacgg ccatgcattg acggtgtaat ggtcggcggc aataggggca
    10741 tggcagccag cgcaataggt atcgtcaagt ttttcagcgt attgccatag tggtttacgg
    10801 tccgctaatg cggaatcggt tagcgcaccc tgtaaagacg cctgacgcca ttgattgccg
    10861 tattcgtcag cctgccattc cccgtttacc gttagcgcct tgataccttc ttcacttaat
    10921 gtggcgagcg ccagccgttg acctgccgcc aggtagagcg tgttttcact gccctgcatt
    10981 tgataaccct gcaaccgaac aattggctgt ttaccactga catcaacgac ggtgagatcg
    11041 gtaccaggag tcacggtagc caggtcgcct atgcgagaag ttttgaaagg ataaatatgt
    11101 gcgccattag taactgaagt agcggcctga ctttccagct catgcgccgc gttgtcatcc
    11161 atttttattt ctggcgggaa atgggcaatg cctttatggc aatcgataca agtttcgccg
    11221 tccttttgcg ctttattatg cattttctgc gcagattcac tttgcgagga aatatccatg
    11281 gcatcaaaag aatggcaact acggcaggtt gcagagtcag ttgcttttaa ttctttccat
    11341 actgtttcgg ccatttcctg gcgatgagct tcgaacttat cgtcactgtc tattttgccg
    11401 ctaacaaatt catgataaat atctttagat gcttttaatt tagcaaataa ataatccatc
    11461 cctgactttg gaatatggca atcggcacat tccgcgcgta tacctttttg gttcgaaaag
    11521 tggacagttc cctgatattc ctcaaaaggt ttactcatcg agtggcagga aaggcaaaat
    11581 gcagtatccg acgttttatg taaggctttt tgcgccagca ataacccgcc gccaccaacc
    11641 acaaccgcta tcagcaaaaa caataaccca atgcgttttt tccctcgcat agattcctct
    11701 aattaaattc caggacaccg ataccattgt ataaataaat atacaacgaa ttg
//
LOCUS       DAHHTG010000127        11421 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.128, whole genome shotgun sequence.
ACCESSION   DAHHTG010000127 DAHHTG010000000
VERSION     DAHHTG010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11421)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11421)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11421
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.128"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            22..837
                     /gene="gspC"
                     /locus_tag="NBI74_003651"
     CDS             22..837
                     /gene="gspC"
                     /locus_tag="NBI74_003651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspC"
                     /protein_id="HCD1933928.1"
                     /translation="MPTLPFPFHLANHNKDAAINILITFISIGLIIFNVNYFHTTIVK
                     NEQIINQPTNAFQSEFSLAALWRNENHTGVKDANPVAVNQETPKLSIALNGIVLTSND
                     ETSFVLINEGNEQKRYSLNEALESAPGTFIRKINKTSVVFETHGHYEKVTLHPGLPDI
                     IKQPDSENQNVLADYIIATPIRDGEQIYGLRLNPRKGLNAFTTSLLQPGDIALRINNL
                     SLTHPDEISQALSLLLTQQSAQFTIRRNGVPRLINVSVAELTGMNGQRNEGTQ"
     gene            821..2773
                     /gene="gspD"
                     /locus_tag="NBI74_003652"
     CDS             821..2773
                     /gene="gspD"
                     /locus_tag="NBI74_003652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system secretin GspD"
                     /protein_id="HCD1933929.1"
                     /translation="MKGLNKITCCLLAALLMPCAGHAENEQYGANFNNADIRQFVEIV
                     GQHLGKTILIDPSVQGTISVRSNDTFSQQEYYQFFLSILDLYGYSVITLDNGFLKVVR
                     SANVKTSPGMIADSSRPGVGDELVTRIVPLENVPARDLAPLLRQMMDAGSVGNVVHYE
                     PSNVLILTGRASTINKLIEVIKRVDVIGTEKQQIIHLEYASAEDLAEILNQLISESHG
                     KSQMPALLSAKIVADKRTNSLIISGPEKARQRITSLLKSLDVEESEEGNTRVYYLKYA
                     KATNLVEVLTGVSEKLKDEKGNSRKPSSTSAMDNVAITADEQTNSLVITADQSVQEKL
                     ATVIARLDIRRAQVLVEAIIVEVQDGNGLNLGVQWANKNVGAQQFTNTGLPVFNAAQG
                     VADYKKNGGITSANPAWDMFSAYNGMAAGFFNGDWGVLLTALASNNKNDILATPSIVT
                     LDNKLASFNVGQDVPVLSGSQTTSGDNVFNTVERKTVGTKLKVTPQVNEGDAVLLEIE
                     QEVSSVDSSSNSTLGPTFNTRTIQNAVLVKTGETVVLGGLLDDFSKEQVSKVPLLGDI
                     PLVGQLFRYTSTERAKRNLMVFIRPTIIRDDDVYRSLSKEKYTRYRQEQQLRIDGKSK
                     ALVGSEDLPVLNENTFNSHAPAPSSR"
     gene            2783..4264
                     /gene="gspE"
                     /locus_tag="NBI74_003653"
     CDS             2783..4264
                     /gene="gspE"
                     /locus_tag="NBI74_003653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system ATPase GspE"
                     /protein_id="HCD1933930.1"
                     /translation="MRIHSPYPASWALAQRIGYLYSEGEIIYLADTPFERLLDIQRQV
                     GQCQTMTSLSQADFEARLEAVFHQNTGESQQIAQDIDQSVDLLSLSEEMPANEDLLNE
                     DSAAPVIRLINAILSEAIKETASDIHIETYEKTMSIRFRIDGVLRTILQPNKKLAALL
                     ISRIKVMARLDIAEKRIPQDGRISLRIGRRNIDVRVSTLPSIYGERAVLRLLDKNSLQ
                     LSLNNLGMTAADKQDLENLIQLPHGIILVTGPTGSGKSTTLYAILSALNIPGRNILTV
                     EDPVEYELEGIGQTQVNTRVDMSFARGLRAILRQDPDVVMVGEIRDTETAQIAVQASL
                     TGHLVLSTLHTNSASGAVTRLRDMGVESFLLSSSLAGIIAQRLVRRLCPQCRQFTPVS
                     PQQAQMFKYHQLAVTTIGTPVGCPHCHQSGYQGRMAIHEMMVVTPELRAAIHENVDEQ
                     ALERLVRQQHNALIKNGLQTVIRGDTSWDEVMRVASATLENKA"
     gene            4261..5457
                     /gene="gspF"
                     /locus_tag="NBI74_003654"
     CDS             4261..5457
                     /gene="gspF"
                     /locus_tag="NBI74_003654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system inner membrane protein
                     GspF"
                     /protein_id="HCD1933931.1"
                     /translation="MNYRYRAMTQDGQKLQGIIDANDERQARLRLREEGLFLLDIRPQ
                     KSSGVKTRRPRISHSELTLFTRQLATLSAAALPLEESLAVIGQQSSNKRLADVLNQVR
                     SAILEGHPLSDALQHFPTLFDSLYRTLVKAGEKSGLLAPVLEKLADYNENRQKIRSKL
                     IQSLIYPCMLTTVAIVVVIILLTAVVPKITEQFVHMKQQLPLSTRILLGLSDTLQRTG
                     PTLLATVFIVAVGFWLWLKRGNNRHRFHAMLLRVALIGPLICAINSARYLRTLSILQS
                     SGVPLLDGMNLSTESLNNLEIRQRLANAAENVRQGNSIHLSLEQTAIFPPMMLYMVAS
                     GEKSGQLGTLMVRAADNQETLQQNRIALTLSIFEPALIITMALIVLFIVVSVLQPLLQ
                     LNSMIN"
     gene            5467..5904
                     /gene="gspG"
                     /locus_tag="NBI74_003655"
     CDS             5467..5904
                     /gene="gspG"
                     /locus_tag="NBI74_003655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system major pseudopilin GspG"
                     /protein_id="HCD1933932.1"
                     /translation="MRATDKQRGFTLLEIMVVIVIIGVLASLVVPNLMGNKEKADKQK
                     AVSDIVALENALDMYKLDNHRYPTTNQGLESLVEAPTLPPLAANYNKEGYIKRLPADP
                     WGNDYVLVNPGEHGAYDLLSAGPDGEMGTEDDITNWGLSKKKK"
     gene            5912..6421
                     /gene="gspH"
                     /locus_tag="NBI74_003656"
     CDS             5912..6421
                     /gene="gspH"
                     /locus_tag="NBI74_003656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspH"
                     /protein_id="HCD1933933.1"
                     /translation="MNQQRGFTLLEMMLVLALVAITASVVLFTYGREDAASTRARETA
                     ARFTAALELAIDRATLSGQPVGIHFSDSAWRIMVPGKTPSAWRWVPLQEDAADESKND
                     WGEELSIQLQPFKPDDSNQPQVVILADGQITPFSLLMANTGTGEPLLTLVSSGSWPLD
                     QTLARDTRP"
     gene            6418..6795
                     /gene="gspI"
                     /locus_tag="NBI74_003657"
     CDS             6418..6795
                     /gene="gspI"
                     /locus_tag="NBI74_003657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417789.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspI"
                     /protein_id="HCD1933934.1"
                     /translation="MNKQSGMTLLEVLLAMSIFTAVALTLMSSMQGQRTAIERMRNET
                     LALWIADNQLQSQDSFDEENTSSSGKELINGEEWNWRSDIHSSKDGTLLERTITVTLP
                     SGQTTSLTRYQSINNKSGQAQDD"
     gene            6788..7375
                     /gene="gspJ"
                     /locus_tag="NBI74_003658"
     CDS             6788..7375
                     /gene="gspJ"
                     /locus_tag="NBI74_003658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspJ"
                     /protein_id="HCD1933935.1"
                     /translation="MINRQQGFTLLEVMAALAIFSMLSVLAFMIFSQASELHQRSQKE
                     IQQFNQLQRTITILDNDLLQLVARRNRSTDKIMVLGEEAIFTTQSRDPLAPLSEAQTL
                     LTVHWYLRNHTLYRAVRTSVDGRKDQPAQAMLEHVESFLLESNSGESQELPLSVTLHL
                     KTQQYGALQRRFALPEQLAREESPAQTQAGNNNHE"
     gene            7368..8351
                     /gene="gspK"
                     /locus_tag="NBI74_003659"
     CDS             7368..8351
                     /gene="gspK"
                     /locus_tag="NBI74_003659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspK"
                     /protein_id="HCD1933936.1"
                     /translation="MNNEQRGVALLIVLMLLALMAALAADMTISFHGQLHRTRQVNHH
                     LQRQYDIELAEKLALASLTQDVKDNDRQTTLQQYWAQPQQLQLENGNTVKWQLRDAQH
                     CFNLNALAKISDAPLASPDFPAQVFSALLINAGIDRGNTDEIVQSIADYIDADDSPRF
                     HGAEDNFYQSQTPPRHSANQMLFLTGELRQIKGITENIYQRLIPYVCVLPTSELSINL
                     NMLTENDIPLFRALFLNNITDADARVLLQKRPREGWLTTDAFLYWAQQDFSGVKPLVA
                     QVKGHLFPYSRYFTLSTESISDEQSQGWQSHIFFNRKQQSAQIYRRTLQLY"
     gene            8366..9529
                     /gene="gspL"
                     /locus_tag="NBI74_003660"
     CDS             8366..9529
                     /gene="gspL"
                     /locus_tag="NBI74_003660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspL"
                     /protein_id="HCD1933937.1"
                     /translation="MPESLMVIRSFSTLRKHWEWMTFSADSVSSVHTLTDDLPLGSLA
                     DQPGAGNVHLLIPPEGLLYRSLTLPNAKYKLTAQTLQWLAEETLPDASQNWHWTVVDK
                     QNESVEVIGIQSEKLSRYLERLHTAGLNVTRVLPDGCYLPWEVDSWTLVNQQTSWLIR
                     SAAHAFNELDEHWLQHLANQFPPENMRCYGVAPHGVAAANPLIQHPEIPSLSLYSADI
                     AFQRYDMLHGVFRKQKTVGKSGKWLTRLAVSCLVLAILSFVGSRGIALWQTLKIEDQL
                     QQQQQETWQRYFPQIKHTHNFRFYFKQQLAQQYPEAVPLLYHLQTLLLEHSELQLMEA
                     NYSQRQKSLTLKMSAKSEANIDRFCELTQSWLPMEKTEKDPVSGVWTVRNSGK"
     gene            9526..9987
                     /gene="gspM"
                     /locus_tag="NBI74_003661"
     CDS             9526..9987
                     /gene="gspM"
                     /locus_tag="NBI74_003661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspM"
                     /protein_id="HCD1933938.1"
                     /translation="MIKSWWAEKSTSEKQIIAALAVLSLGVFCWLAVIKPIDTYIEEH
                     QSHAQKIKKDIKWMQDQASTHGLLGHPALTQPIKNILLEEAKRENLTITLENGPDNTL
                     TINPVTAPLENVSRWLTTAQITYGIVIEDLQFTLAGNEEITLKHLSFREQQ"
     gene            9987..10664
                     /gene="gspO"
                     /locus_tag="NBI74_003662"
     CDS             9987..10664
                     /gene="gspO"
                     /locus_tag="NBI74_003662"
                     /EC_number="3.4.23.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase GspO"
                     /protein_id="HCD1933939.1"
                     /translation="MTMLLPLFILVGFIAGYFVNAMAYHLSPLEDKTALTFRQVLVHF
                     WQKKYAWHDTVPLILCVAAAIACALAPFTPIVTGALFLYFCFALTLSVIDFRTQLLPD
                     KLTLPLLWLGLVFNAQSGLIDLHDAVYGAVAGYGVLWCVYWGVWLVCHKEGLGYGDFK
                     LLAAAGAWCGWQTLPMILLIASLGGIGYAIVSQLLQRRTITTIAFGPWLALGSMINLG
                     YLAWISY"
     gene            complement(10693..11169)
                     /gene="bfr"
                     /locus_tag="NBI74_003663"
     CDS             complement(10693..11169)
                     /gene="bfr"
                     /locus_tag="NBI74_003663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HCD1933940.1"
                     /translation="MKGDTKVINYLNKLLGNELVAINQYFLHARMFKNWGLKRLNDVE
                     YHESIDEMKHADRYIERILFLEGLPNLQDLGKLNIGEDVEEMLRSDLALELDGAKNLR
                     EAIGYADSVHDYVSRDMMIEILRDEEGHIDWLETELDLIQKMGLQNYLQAQIREEG"
     gene            complement(11241..>11421)
                     /gene="bfd"
                     /locus_tag="NBI74_003664"
     CDS             complement(11241..>11421)
                     /gene="bfd"
                     /locus_tag="NBI74_003664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="bacterioferritin-associated ferredoxin"
                     /protein_id="HCD1933941.1"
                     /translation="CNGISDKKIRQAVRQFSPHSFQQLKKFIPVGNQCGKCVRAAREV
                     MEDELMQLPEFKESA"
BASE COUNT         2993 a         2926 c         2702 g         2800 t
ORIGIN      
        1 tattttatta gaacatacat cgtgcccaca ctacctttcc ccttccatct ggcgaatcat
       61 aataaagatg ctgccatcaa tatattgatt acatttataa gcatagggct gattattttt
      121 aatgtgaatt atttccatac aactatagtt aaaaatgaac agatcattaa tcaaccaaca
      181 aatgcatttc agtctgaatt cagccttgcc gctttatggc gcaacgaaaa tcatactggg
      241 gtaaaagatg caaatcccgt ggcagttaat caggaaacac ctaagctctc tatagcgctt
      301 aatggcattg tactcacatc gaatgatgag acttcttttg tcttaattaa tgaggggaac
      361 gaacaaaagc gatactccct caacgaagcc cttgagtcag cgccgggaac gtttattaga
      421 aaaataaata aaaccagcgt tgtgtttgaa actcacgggc attacgaaaa agtaactctc
      481 catccagggc tacctgacat catcaagcag cctgattcgg aaaatcaaaa tgtactcgcc
      541 gattatatta tcgccacacc tatacgtgat ggagaacaga tatacggtct tcgattgaat
      601 cctcgtaagg gtctcaatgc atttaccacc agtttgctgc aacctgggga tatcgccctc
      661 aggatcaata atctttcgct gacccaccct gatgagattt cacaggcatt aagtttactg
      721 ctaactcaac aaagtgctca gtttacaatt cgtcgcaacg gcgtaccccg cttgataaat
      781 gtttccgtcg cggaacttac aggaatgaat ggacagcgca atgaagggac tcaataaaat
      841 cacctgctgc ttgctggcag cattactcat gccttgtgca ggacacgctg agaacgaaca
      901 atacggcgct aacttcaata acgccgatat ccgccagttc gtggaaatag tgggtcagca
      961 tcttggcaaa acgatcctga tcgacccttc ggtacaggga accatttccg tacgcagtaa
     1021 tgatacgttt agccaacagg agtactacca gttcttttta agtattcttg atctttacgg
     1081 ttattccgtg atcacgctgg acaatggttt tctgaaagtg gtgcgctcag ctaatgtaaa
     1141 aacatcgcca gggatgattg ctgacagttc tcgtccaggc gtaggtgatg agttggtcac
     1201 ccgaatcgta ccgcttgaga acgttcctgc tcgtgacctg gctcccctgc tccgccagat
     1261 gatggatgcg ggtagcgtcg gtaatgttgt gcattatgaa ccctccaacg ttcttattct
     1321 gaccggtcgt gcctccacca ttaataaact gattgaagtc ataaagcgcg ttgatgtcat
     1381 cggcacagag aagcagcaaa ttattcatct ggaatatgca tcagcggaag atctcgccga
     1441 gattcttaat caattaatca gcgaaagcca cggtaaaagc cagatgcctg ccctcctttc
     1501 cgcgaagatt gtggcggata agcgaaccaa ctctcttatc atcagcggac cggaaaaagc
     1561 acgccagcgc ataacttcat tactgaaaag ccttgatgta gaagagagcg aggaaggaaa
     1621 tacccgggtc tattacctga aatatgctaa agccacgaat ctggtggaag tgctaaccgg
     1681 tgtttccgaa aagctgaaag atgaaaaagg gaattcgcgt aagccttctt ctacttctgc
     1741 gatggataat gtggccatta ccgccgatga acagaccaac tcgttggtca ttaccgctga
     1801 ccagtccgtc caggagaaac tcgccacggt aattgcgcgt ctggatattc gccgtgcaca
     1861 ggtgctggtt gaggcaatca tcgtcgaagt tcaggatgga aatggactca acctcggcgt
     1921 gcaatgggcg aataaaaacg ttggcgcaca gcaatttacc aatacgggat taccggtttt
     1981 taacgctgcg caaggtgtag ctgattataa aaagaatggc gggatcacca gcgcgaatcc
     2041 tgcctgggat atgtttagcg cctacaatgg catggctgca ggcttcttca atggcgactg
     2101 gggcgtattg ttgactgcgc tggccagtaa caataaaaat gacatcctcg ccaccccgag
     2161 catcgtaacg ctggataata aactcgcgtc cttcaacgtt ggtcaggatg tgccggtgct
     2221 atccggctct cagaccactt ctggggataa cgtctttaat actgtcgaac gcaaaacggt
     2281 gggcacaaaa ctcaaagtca ctccgcaggt caatgaaggc gacgcggtac tgctcgaaat
     2341 agaacaggag gtttccagcg ttgactcttc gtctaattcg acgctcggcc cgacatttaa
     2401 cacccgtact attcaaaacg ccgtgctggt taaaaccggt gaaacggtgg tcctgggcgg
     2461 attgctggat gatttttcta aagagcaagt gtcaaaggtt cctctgcttg gcgatattcc
     2521 tttagtggga caactcttcc gctatacctc gaccgagcgc gctaaacgca acctgatggt
     2581 attcatccgc ccgacgatta tccgtgacga tgatgtttat cgctcactgt caaaagagaa
     2641 atacacccgc taccgtcagg agcaacagct gcgaatcgac gggaaatcaa aagcgctggt
     2701 tggctcggaa gatttgccgg tgctgaatga gaacacgttc aacagtcacg cccctgcgcc
     2761 atcgtcacgg tgaggcattc ttatgagaat tcactcaccg taccccgcca gttgggcgct
     2821 ggcacaacga attggttatc tctattcaga gggcgagatt atttatctcg ccgatacgcc
     2881 attcgaacgg ttactcgata ttcaacgtca agttggtcag tgccagacca tgaccagctt
     2941 gtcacaggct gattttgaag cccgactgga agcggtgttc catcagaata ccggtgagtc
     3001 gcaacagatt gcgcaggata tcgatcaatc cgtcgatctt ctctcgcttt cggaagagat
     3061 gcccgcaaat gaagatctcc tgaatgaaga ttcagcggca ccggttatcc gcttgatcaa
     3121 tgctattttg agtgaggcca tcaaagaaac cgcctccgat atccacattg aaacctatga
     3181 aaaaacaatg tcgatccgtt ttcgcatcga cggcgttttg cggacaattt tacagcccaa
     3241 caaaaaactg gcggcactgc ttatctcccg aattaaggtc atggctcgtc ttgatatcgc
     3301 cgaaaaacgt attccacagg atggaaggat cagtttgcgt atcgggcgac gtaacataga
     3361 tgtccgcgta tccacactgc catccatcta tggtgaacgc gctgtgctcc gtctgctgga
     3421 taaaaacagc ctccagcttt cattgaacaa cctggggatg acggcagcgg ataaacagga
     3481 tttagaaaat ctcattcagc ttccgcacgg tattatcctg gtgacagggc cgacaggctc
     3541 cggtaaaagc accacgctct acgccatcct ttcggcgctt aatatccctg gccgtaatat
     3601 tctgacggta gaagatcccg tggaatatga gctggaaggc attggacaaa cgcaggtgaa
     3661 tacccgtgtg gatatgtctt tcgctcgcgg cctgcgcgcc atacttcgcc aggacccgga
     3721 tgtcgtcatg gtgggggaaa ttcgtgatac agaaaccgcg caaattgcgg ttcaggcctc
     3781 gctcacaggc catctggtac tctcaacact ccacactaac agtgcatcag gcgcagtcac
     3841 ccggctacgc gacatgggcg tcgaatcatt cctgctttca tcttcactgg cagggattat
     3901 cgcgcaacgt ctggttcgtc gtctgtgtcc gcaatgccga caattcacgc ccgtatcgcc
     3961 ccagcaagcg cagatgttta aatatcatca gctcgcggtg acaacaattg gcactcccgt
     4021 aggttgccct cattgccatc aatccggtta tcaggggcgc atggcgatcc acgaaatgat
     4081 ggtggtgacg ccggaattgc gggccgctat tcatgaaaat gtggatgaac aagcactgga
     4141 gcgactggtc cggcaacaac acaatgcctt aatcaaaaat ggcctgcaaa cagtgatacg
     4201 cggtgacacc tcctgggatg aggttatgcg cgtcgccagt gctacgctgg agaacaaagc
     4261 atgaattatc gctatcgcgc catgacccag gatggtcaaa aattgcaggg gatcattgat
     4321 gctaacgatg aacgtcaggc acgactgcgg ctgcgtgaag aagggctttt cctgttggat
     4381 attcgccccc aaaaaagttc gggagtaaaa acacgtcgcc cgaggatcag ccatagtgaa
     4441 ctgacgcttt tcacccggca gttggcaacc ttaagcgcag cggcattacc cctggaagag
     4501 agccttgccg taattggtca acaaagcagt aataaacgac tggctgacgt gttaaatcag
     4561 gtacgcagcg ccatccttga agggcatccc ctttcagatg cattacagca tttccccacg
     4621 cttttcgatt cgctctaccg taccctggta aaagcgggcg aaaagagcgg tctgctggcc
     4681 ccggtgttgg aaaagctggc tgattacaat gaaaaccggc agaaaatccg cagcaagctc
     4741 attcagtcac tgatctaccc ctgcatgctc actacggtgg cgattgtggt cgtgattatt
     4801 ctcctcactg ctgtcgtgcc caaaattacc gaacagttcg tgcatatgaa gcagcaactg
     4861 ccactgagta cacgcattct tttaggtctg agcgacacgt tgcaacgtac cggcccgaca
     4921 ttattagcga cagtatttat tgtcgctgtg ggtttctggc tctggttaaa acgcggcaat
     4981 aaccgccacc gttttcatgc catgttactg cgcgttgcgc tcatcggccc gctgatttgc
     5041 gccattaaca gcgcacgcta tctccgcact ttaagtattt tgcaatccag cggcgtccct
     5101 ctgctggatg ggatgaattt gtccaccgaa agcctcaaca acctcgaaat tcgccagcgc
     5161 ctggcaaatg cggcagaaaa cgttcgccag ggtaacagca ttcatctttc actggaacaa
     5221 accgcaattt tcccgccgat gatgctctac atggtggcct ctggcgaaaa aagcgggcaa
     5281 ctcggcacgt taatggtcag agccgcagat aaccaggaga cactccaaca aaatcggatc
     5341 gccttaacgc tctccatctt cgagccagca ctcattatta cgatggcact gatcgtcctg
     5401 tttattgtcg tgtcggtact ccaacctctt cttcaactta actcaatgat taattaagga
     5461 aatcatatgc gcgcaacgga taagcaacgc ggttttacat tactggaaat tatggtggta
     5521 attgtcatca ttggcgtact cgccagcctg gtggttccca acctgatggg caataaagaa
     5581 aaggcggata aacaaaaagc cgtcagcgat attgtggcgc tggaaaacgc cctggatatg
     5641 tacaagctcg acaatcatcg ctatccaacc acaaatcagg ggcttgaatc cttagtagaa
     5701 gcgccgacac tgccaccgct ggccgcaaac tataacaagg aaggttatat caagcgtctg
     5761 cctgccgatc cctggggcaa tgattatgtc ctcgttaatc ctggtgaaca tggtgcatac
     5821 gatctgcttt cagcaggacc cgatggtgaa atgggaaccg aggacgacat caccaactgg
     5881 ggtttgagca agaagaaaaa gtaagtcagc gatgaatcag caacgcgggt ttaccctgct
     5941 ggagatgatg ctggtgctgg cgctggtcgc tatcacggca agtgtggtgc tcttcacata
     6001 tggtcgggag gatgccgcca gcactcgtgc gcgcgaaaca gcggcgcggt ttaccgctgc
     6061 acttgaatta gccatcgacc gcgccacgct cagtggtcag cctgtcggta tccatttctc
     6121 tgattcagca tggcgcatta tggtaccggg caaaacacca tcagcctggc gctgggttcc
     6181 attacaagaa gatgccgcag acgagagcaa aaatgactgg ggtgaagaac tctcaattca
     6241 acttcagcca tttaaacctg atgacagcaa ccaaccgcag gtggtgattc tggccgacgg
     6301 acaaatcacg cccttctctt tgttgatggc taataccggg accggtgagc cgctcctgac
     6361 gttagtcagt tcaggctcat ggcccctgga tcaaacgctc gccagagata cccgcccatg
     6421 aacaaacaat cagggatgac actgcttgaa gtcttactgg cgatgagtat tttcaccgct
     6481 gttgcgctga ctttaatgag cagtatgcaa gggcaacgaa ctgccatcga gcggatgcgt
     6541 aacgagacgc tggcattgtg gatagcggat aaccagcttc agtcacaaga ttcattcgac
     6601 gaggaaaaca cgtccagttc tggcaaagag ttaataaatg gcgaagagtg gaactggcgc
     6661 agcgatatcc actcaagcaa agacggcacg cttctggaac gcaccataac ggtgacatta
     6721 cccagcggcc agacaacctc gcttacgcgc tatcaaagca tcaataataa atccgggcag
     6781 gcacaagatg attaatcgcc agcagggttt cacattgctg gaagtcatgg cggcgctggc
     6841 gattttctca atgcttagcg tgctggcgtt tatgattttt tctcaagctt ccgagttgca
     6901 tcaacgttca cagaaagaaa tccagcagtt caatcagcta cagcgcacca tcaccattct
     6961 tgataacgat ctcctgcaac tggtcgcccg gcggaatcgg agtacggaca aaatcatggt
     7021 gttgggtgaa gaagcgattt ttaccacgca aagtcgcgat ccgctggccc ctctcagtga
     7081 agcacaaacc ttactcactg ttcactggta tctgcgaaat catacgctct accgtgctgt
     7141 tcgtacttct gtggatggca ggaaggatca acccgcccag gcgatgctgg aacatgtcga
     7201 gagctttctt ctggaaagta acagcggaga aagccaggag cttcctctct cagtcacgtt
     7261 acatctgaag actcagcaat atggggcgtt acagcgccgt tttgcattac ctgagcagtt
     7321 agcgagagaa gagtcgccag ctcagacgca ggccgggaat aataaccatg aataatgaac
     7381 aacgcggcgt agcactgctc attgtactca tgttactggc gctcatggct gctctggctg
     7441 cagacatgac gatcagtttt cacggccagc ttcatcgaac ccgccaggta aaccatcatt
     7501 tgcaacggca atatgatatt gagctggcgg agaaacttgc gcttgccagc ctcacccagg
     7561 acgttaaaga taacgacaga caaaccacgt tgcagcagta ctgggcacaa ccgcaacagt
     7621 tgcagctgga aaatggcaat acagtgaagt ggcagttacg cgatgcccag cactgtttta
     7681 atctcaatgc cttagcaaaa atctctgacg ccccgctggc ttcccctgat ttccccgccc
     7741 aggtattcag tgccctgctg attaacgccg gtatcgatcg cggaaacacc gatgagatcg
     7801 tgcaatcaat cgctgactat attgatgccg atgattcgcc acgctttcac ggcgcagaag
     7861 ataactttta ccaaagccaa acgccgcctc gccatagcgc caatcaaatg ctttttctga
     7921 ccggtgaatt gcgtcagata aaaggcataa ccgaaaacat ctatcagcgg cttatcccgt
     7981 atgtctgcgt tctcccgacc tcggaactta gcatcaatct taatatgcta acggagaacg
     8041 atattccgct cttcagagca ttattcctca ataacatcac ggatgctgac gctcgggtct
     8101 tattacaaaa aaggcccagg gaaggctggc taaccacaga tgcatttctt tactgggcgc
     8161 agcaggattt ctccggcgtg aagcccctgg ttgctcaggt gaaagggcat ctctttccct
     8221 atagccgcta ttttacgctg agtacggaaa gtatcagcga cgaacaatcc cagggatggc
     8281 aaagccatat ttttttcaac cgtaaacagc agagcgccca aatctatcgg cgtacgctgc
     8341 aactctacta atcataagga tcgtgatgcc tgaatcgttg atggtaatac gttctttctc
     8401 gacgctgcgt aagcattggg aatggatgac gttctccgca gatagcgtat cttcggtaca
     8461 tacgctcaca gacgatcttc cgctggggtc gctggccgat cagcccggtg ccggaaatgt
     8521 tcaccttttg atcccacctg agggtctttt atatcgttcc ctcacgctgc ccaacgccaa
     8581 atacaaactc accgcgcaaa cattacagtg gttggcggag gagacattac ccgacgccag
     8641 ccaaaattgg cactggacgg ttgtcgataa acaaaacgaa agtgtcgagg tgataggcat
     8701 tcagtcggag aaactcagtc gttatcttga gcgtttacat actgccggac tcaacgtgac
     8761 gcgggttttg ccagatggct gctatcttcc ctgggaagta gatagctgga ctctggttaa
     8821 ccagcaaaca agctggctta ttcgcagtgc cgcacatgcc ttcaacgaac tggatgaaca
     8881 ctggttacag catcttgcta accagtttcc gcctgagaat atgcgttgtt atggtgtagc
     8941 gcctcacggc gttgcagcgg ccaatccact tatccagcat cctgaaatac catcactgag
     9001 tttgtattct gccgatattg cattccagcg ctatgacatg ctgcatggtg tttttcgtaa
     9061 acagaaaact gtaggcaaat cgggaaaatg gctcacccgc ctggcagtaa gctgtctcgt
     9121 tctcgccatt ctttcttttg ttggcagccg gggcatcgcg ctctggcaaa cgcttaagat
     9181 tgaagatcaa cttcagcaac aacagcaaga aacctggcaa cgttatttcc cgcagatcaa
     9241 acacacccac aactttcgtt tttactttaa gcagcaactc gctcagcaat atcctgaagc
     9301 tgtaccgttg ctctatcatt tacaaacact tctgcttgaa cactctgaac tgcagttaat
     9361 ggaggcgaac tatagccaga ggcaaaagtc gctgacctta aaaatgagtg ctaagagtga
     9421 agccaatatc gatcgtttct gtgagttaac acagtcctgg cttccgatgg aaaaaacaga
     9481 aaaagatccg gtcagtggtg tatggacagt aaggaactca gggaaatgat taaatcatgg
     9541 tgggcagaaa aatccacatc ggaaaaacag atcattgcgg cactggcggt tctctcgctc
     9601 ggcgtttttt gttggcttgc ggtgataaaa ccgatagata cctacattga agagcatcag
     9661 tcccacgcgc aaaaaattaa gaaggacatc aaatggatgc aggatcaagc gagcacccat
     9721 ggtttactcg gtcatccagc gcttacgcag cccatcaaaa atatccttct cgaagaagca
     9781 aaacgtgaga atttgactat cacgctggaa aacggtccgg acaatactct gacaattaat
     9841 cctgtcacag ctcccctcga aaacgtctcc cgctggctca ctacggcaca gataacatac
     9901 ggtattgtta ttgaagacct tcagtttact cttgccggaa acgaagagat caccctcaag
     9961 catttgtcct tcagggagca acaataatga caatgttact gccgctcttc attctggttg
    10021 gttttattgc aggttatttt gttaatgcca tggcctatca cctctcgcct ttggaggata
    10081 aaacggcgtt aacgtttcgc caggtattgg ttcatttctg gcaaaaaaaa tatgcctggc
    10141 atgatacagt gcccctgata ctttgcgttg ctgctgctat cgcctgcgcc ctggcacctt
    10201 tcacgcccat cgtgactggc gcactctttc tctatttctg tttcgcactc acgctcagtg
    10261 ttattgattt tcgcactcag ctcctgcccg acaaactcac cttaccgctg ctctggcttg
    10321 gcttggtatt taatgcgcag tctggattaa ttgatttaca tgatgcggtt tacggcgcgg
    10381 tcgctggcta tggggtgctg tggtgtgttt actggggcgt ctggttagtt tgtcacaaag
    10441 agggattggg ctacggtgat ttcaagctac tggccgctgc aggcgcgtgg tgtggctggc
    10501 aaacgttgcc aatgatactg ctgattgcct cgctgggtgg cattggttac gccatcgttt
    10561 cacaacttct gcaacgccgg actataacca ctatcgcatt cggcccgtgg cttgcgctcg
    10621 gcagcatgat aaacctggga tatctggcct ggatctctta ttaaccggga gggttctccc
    10681 tcccgacacg gctcaacctt cttcgcgaat ctgtgcttgc agataatttt gcagtcccat
    10741 cttctgaatc agatcaagtt ccgtttccag ccagtcgata tggccttctt catcgcgcaa
    10801 aatttctatc atcatatcgc ggctgacgta atcatgaacg ctatcggcat aaccaattgc
    10861 ctcacgcaaa ttcttcgcgc catccagctc aagtgccaga tcagaacgca gcatttcctc
    10921 aacatcttca ccaatgttca gtttgcccag gtcctgtaag tttggaagac cttccagaaa
    10981 aagaatgcgc tctatataac gatcggcgtg tttcatctca tcaatggatt catgatactc
    11041 cacatcattg agacgtttga gaccccagtt tttaaacatc cgggcatgaa gaaagtactg
    11101 attgattgcg acaagctcat ttcccaacag tttgttgaga taatttataa ctttagtatc
    11161 acctttcatt ttttgactcc ttcgcttcca ctctttgaag cgtagaacgg cttacgagta
    11221 agtcaaaaaa cacactccgt ttatgcggac tccttaaact ccggcagttg cattaattca
    11281 tcctccatca cttcacgcgc ggcccggaca catttaccgc actgattgcc gaccggaatg
    11341 aattttttta attgctggaa cgagtggggg gaaaactggc gcacagcctg acgaattttt
    11401 ttatcgctga taccattaca a
//
LOCUS       DAHHTG010000128        11396 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.129, whole genome shotgun sequence.
ACCESSION   DAHHTG010000128 DAHHTG010000000
VERSION     DAHHTG010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11396)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11396)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11396
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.129"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            61..420
                     /locus_tag="NBI74_003665"
     CDS             61..420
                     /locus_tag="NBI74_003665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001331208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1889 family protein"
                     /protein_id="HCD1933942.1"
                     /translation="MQIKVIYSLIDNMVNFKDKNMPAVIDKALDFIGAMDVSAPTPSS
                     MNESTAKGIFKYLKELGVPASAADITARADQEGWNPGFTEKMVGWAKKMESGERSVIK
                     NPEYFSTYMQEELKALV"
     gene            complement(540..719)
                     /locus_tag="NBI74_003666"
     CDS             complement(540..719)
                     /locus_tag="NBI74_003666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaH family protein"
                     /protein_id="HCD1933943.1"
                     /translation="MFAGLPSLTHEQQQKAVERIQELMAQGMSSGQAIALVAEELRAN
                     HSGERIVARFEDEDE"
     gene            793..2154
                     /gene="pabB"
                     /locus_tag="NBI74_003667"
     CDS             793..2154
                     /gene="pabB"
                     /locus_tag="NBI74_003667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 1"
                     /protein_id="HCD1933944.1"
                     /translation="MKTLSPAVITLPWRQDAAEFYFSRLSHLPWAMLLHSGYADHPYS
                     RFDIVVAEPICTLTTFGKETVVSESEKRTTTTDDPLQVLQQVLDRADIHPAHNEDLPF
                     QGGALGLFGYDLGRRFESLPEIAEQDIVLPDMAVGIYDWALVIDHQRQTVSLLSHNDV
                     NARRAWLENQQFSLQEDFALTSGWQSNMTRELYGEKFRKVQEYLHSGDCYQVNLAQRF
                     HATYSGDEWQAFLQLNQANRAPFSAFLRLEQAAILSLSPERFILCDNSEIQTRPIKGT
                     LPRLPDLQEDSKQAEKLANSTKDRAENLMIVDLMRNDIGRVAVAGSVKVPELFVVEPF
                     PAVHHLVSTITAQLPEQLHASDLLRAAFPGGSITGAPKVRAMEIIDELEPQRRNAWCG
                     SIGYLSFCGNMDTSITIRTLTAINGQIYCSAGGGIVADSQEEAEYQETFDKVNKILRQ
                     LEK"
     gene            2158..2736
                     /locus_tag="NBI74_003668"
     CDS             2158..2736
                     /locus_tag="NBI74_003668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HCD1933945.1"
                     /translation="MEYRSLTLDDFLSRFQLLRPQINRETLNHRQAAVLIPIVRRPQP
                     GLLLTQRSIHLRKHAGQVAFPGGAVDDTDASVIAAALREAEEEVAIPPSAVEVIGVLP
                     PVDSVTGYQVTPVVGIIPPDLPYRASEDEVSAVFEMPLAQALHLGRYHPLDIYRRGDS
                     HRVWLSWYEQYFVWGMTAGIIRELALQIGVKP"
     gene            2920..4284
                     /gene="sdaA"
                     /locus_tag="NBI74_003669"
     CDS             2920..4284
                     /gene="sdaA"
                     /locus_tag="NBI74_003669"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HCD1933946.1"
                     /translation="MISLFDMFKVGIGPSSSHTVGPMKAGKQFVDDLVEKGLLDSVTR
                     VAVDVYGSLSLTGKGHHTDIAIIMGLAGNEPATVDIDSIPGFIRDVEERERLLLAQGR
                     HEVDFPRDNGMRFHNGNLPLHENGMQIHAYNGDEVVYSKTYYSIGGGFIVDEEHFGQD
                     AANEVSVPYPFKSATELLAYCNETGFSLSGLAMQNELALHSKKEIDEYFAHVWQTMQA
                     CIDRGMNTEGVLPGPLRVPRRASALRRMLVSSDKLSNDPMNVIDWVNMFALAVNEENA
                     AGGRVVTAPTNGACGIVPAVLAYYDHFIESVSPDIYTRYFMAAGAIGALYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGGSPEQVCVAAEIGMEHNLGLTCDPVAGQVQVPC
                     IERNAIASVKAINAARMALRRTSAPRVSLDKVIETMYETGKDMNAKYRETSRGGLAIK
                     VQCD"
     gene            4415..6013
                     /locus_tag="NBI74_003670"
     CDS             4415..6013
                     /locus_tag="NBI74_003670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416329.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HCD1933947.1"
                     /translation="MQKAQRIIKTYRRNRMIVCTICALVTLASTLSVRFISQRNLNQQ
                     RVVQFANHAVEELDKVLLPLQAGSEVLLPLIGLPCSVAHLPLRKQAAKLQTVRSIGLV
                     QDGTLYCSSIFGYRNVPVVDILAELPAPQPLLRLTTDRALIKGSPVLIQWTPAAGSSN
                     AGVMEMINIDLLTAMLLEPQLPQISSASLTVDSRHLLYGNGLVDSLPQPENNENYQVS
                     SQRFPFTINVNGPGATALAWHYLPTQLPLAVLLSLLVGYIAWLATAYRMSFSREINLG
                     LAQHEFELFCQPLLNARSQQCIGVEILLRWNNPRQGWISPDVFIPIAEEHHLIVPLTR
                     YVMAETIRQRHVFPMSSQFHVGINVAPSHFRRGVLIKDLNQYWFSAHPIQQLILEITE
                     RDALLDVDYRIARELHRKNVKLAIDDFGTGNSSFSWLETLRPDVLKIDKSFTAAIGSD
                     AVNSTVTDIIIALGQRLNIELVAEGVETQEQAKYLRSHGVHILQGYLYAQPMPLRDFP
                     KWLAGSQPPPARHNGHITPIMPLR"
     gene            complement(6017..7573)
                     /gene="yoaE"
                     /locus_tag="NBI74_003671"
     CDS             complement(6017..7573)
                     /gene="yoaE"
                     /locus_tag="NBI74_003671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family cation transport protein YoaE"
                     /protein_id="HCD1933948.1"
                     /translation="MEFLMDPSIWAGLLTLVVLEIVLGIDNLVFIAILADKLPPKQRD
                     KARLLGLSLALIMRLGLLSLISWMVTLTKPLFTVMDFSFSGRDLIMLFGGIFLLFKAT
                     TELHERLENRDHDSGHGKGYASFWVVVTQIVILDAVFSLDAVITAVGMVNHLPVMMAA
                     VVIAMAVMLLASKPLTRFVNQHPTVVVLCLSFLLMIGLSLVAEGFGFHIPKGYLYAAI
                     GFSIIIEVFNQIARRNFIRHQSTLPLRARTADAILRLMGGKRQANVQHDADNPMPMPI
                     PEGAFAEEERYMINGVLTLASRSLRGIMTPRGEISWVDANLGVDEIREQLLSSPHSLF
                     PVCRGELDEIIGIVRAKELLVALEEGVDVAAIASASPAIIVPETLDPINLLGVLRRAR
                     GSFVIVTNEFGVVQGLVTPLDVLEAIAGEFPDADETPEIITDGDGWLVKGGTDLHALQ
                     QALDVEHLADDDDIATVAGLVISANGHIPRVGDVIDVGPLHITIIEANDYRVDLVRIV
                     KEQPAHDEDE"
     gene            8037..9008
                     /gene="manX"
                     /locus_tag="NBI74_003672"
     CDS             8037..9008
                     /gene="manX"
                     /locus_tag="NBI74_003672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HCD1933949.1"
                     /translation="MTIAIVIGTHGWAAEQLLKTAEMLLGEQENVGWIDFVPGENAET
                     LIEKYNAQLAKLDTTKGVLFLVDTWGGSPFNAASRIVVDKEHYEVIAGVNIPMLVETL
                     MARDDDPSFDELVALAVETGREGVKALKAKPIEKAAPAPAAAAPKVAPTPAKPMGPND
                     YMVIGLARIDDRLIHGQVATRWTKETNVSRIIVVSDEVAADTVRKTLLTQVAPPGVTA
                     HVVDVAKMIRVYNNPKYAGERVMLLFTNPTDVERLVEGGVKITSVNVGGMAFRQGKTQ
                     VNNAVSVDEKDIEAFKKLNARGIELEVRKVSTDPKLKMMDLISKIDK"
     gene            9071..9871
                     /gene="manY"
                     /locus_tag="NBI74_003673"
     CDS             9071..9871
                     /gene="manY"
                     /locus_tag="NBI74_003673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIC"
                     /protein_id="HCD1933950.1"
                     /translation="MEITTLQIVLVFIVACIAGMGSILDEFQFHRPLIACTLVGIVLG
                     DMKTGIIIGGTLEMIALGWMNIGAAVAPDAALASIISTILVIAGHQSIGAGIALAIPL
                     AAAGQVLTIIVRTITVAFQHAADKAADNGNLTAISWIHVSSLFLQAMRVAIPAVIVAL
                     SVGTSEVQNMLNAIPEVVTNGLNIAGGMIVVVGYAMVINMMRAGYLMPFFYLGFVTAA
                     FTNFNLVALGVIGTVMAVLYIQLSPKYNRVAGAPAQAAGNNDLDNELD"
     gene            9884..10735
                     /gene="manZ"
                     /locus_tag="NBI74_003674"
     CDS             9884..10735
                     /gene="manZ"
                     /locus_tag="NBI74_003674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020804907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IID"
                     /protein_id="HCD1933951.1"
                     /translation="MVDTTQTTTEKKLTQSDIRGVFLRSNLFQGSWNFERMQALGFCF
                     SMVPAIRRLYPENNEARKQAIRRHLEFFNTQPFVAAPILGVTLALEEQRANGAEIDDG
                     AINGIKVGLMGPLAGVGDPIFWGTVRPVFAALGAGIAMSGSLLGPLLFFILFNLVRLA
                     TRYYGVAYGYSKGIDIVKDMGGGFLQKLTEGASILGLFVMGALVNKWTHVNIPLVVSR
                     ITDQTGKEHVTTVQTILDQLMPGLVPLLLTFACMWLLRKKVNPLWIIVGFFVIGIAGY
                     ACGLLGL"
     gene            10790..11248
                     /locus_tag="NBI74_003675"
     CDS             10790..11248
                     /locus_tag="NBI74_003675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF986 domain-containing protein"
                     /protein_id="HCD1933952.1"
                     /translation="MTITDLVLILFIAALLAFAIYDQFIMPRRNGPTLLAIPLLRRGR
                     IDSVIFVGLIVILIYNNVTNHGALITTWLLSALALMGFYIFWIRVPKIIFKQKGFFFA
                     NVWIEYSRIKAMNLSEDGVLVMQLEQRRLLIRVRNIDDLEKIYKLLVSTQ"
BASE COUNT         2816 a         2857 c         2844 g         2879 t
ORIGIN      
        1 aaacacctga atgtcaacag gttaactcgt gccatatcgt tataatccct ttgatattag
       61 atacaaatta aggtcatata tagccttatc gataacatgg ttaattttaa ggataagaat
      121 atgcctgctg taatagataa agccctggat ttcattggtg ccatggatgt atcagcgcca
      181 acaccaagtt cgatgaatga aagtacggcg aagggaatct ttaaatattt aaaagaactg
      241 ggagtacccg cgagtgccgc tgatattacg gcgcgagctg accaggaagg ctggaaccct
      301 ggtttcacgg aaaaaatggt tggatgggca aaaaaaatgg agtctggtga acgctctgtg
      361 attaaaaatc ctgaatactt ttcaacatat atgcaggaag aactaaaagc actggtttga
      421 gttaaattta catcagcata aatgggtcag gacgctttta atcacatata aaaagcgtcc
      481 tttttctcac aatcaaataa tcatcggctt ctataacgaa agcgatacgg attagtgtgt
      541 tactcgtctt catcctcaaa acgcgccacg attcgctcgc cggaatggtt ggcgcgcagc
      601 tcttctgcca ccaacgcgat tgcctggccg ctgctcatcc cttgtgccat cagttcctgg
      661 atccgctcga cggctttttg ctgctgttca tgggtgagtg aaggtaaacc tgcaaacatt
      721 gttaactcct gctaaattgt tggcgctaat tatttcatgc tacccggcac atagccagta
      781 gagtcaggac tgatgaagac gttatctccc gctgtgatta ctttaccctg gcgtcaggac
      841 gccgctgaat tttatttctc ccgcttaagc cacctgccgt gggcgatgct tttacactcc
      901 ggctatgccg atcatccgta tagccgcttt gatattgtgg tcgccgagcc gatttgcact
      961 ttaaccactt tcggtaaaga aaccgttgtt agtgaaagcg aaaaacgcac aacgaccact
     1021 gatgacccgc tacaggtgct ccagcaggtg ctggatcgcg cagacattca cccagcgcat
     1081 aacgaagatt tgccatttca gggcggcgcg ctggggttgt ttggctacga tctgggccgc
     1141 cgttttgagt cactgccaga aattgcggaa caagatatcg ttctgccgga tatggcagtg
     1201 ggtatctacg actgggcgct ggttatcgac caccagcgtc aaacagtttc tttgctgagt
     1261 cataatgatg tcaatgcccg tcgggcctgg ctggaaaacc agcaattctc cctgcaggaa
     1321 gatttcgcgc ttacttccgg ctggcaatcc aatatgaccc gcgagctgta cggcgaaaaa
     1381 tttcgcaaag tacaggaata tctgcacagc ggtgattgct atcaggtgaa tctcgcccag
     1441 cgttttcatg cgacctattc tggcgatgaa tggcaggcat tccttcagct taatcaggcc
     1501 aaccgcgcgc cgtttagcgc ttttttacgt cttgaacagg ctgctatttt aagcctttcg
     1561 ccagagcggt ttattctttg tgataatagt gaaattcaga cccgcccgat taaaggcacg
     1621 ctaccacgcc tgcccgatct tcaggaagat agcaaacaag cagaaaaact ggcgaactca
     1681 acgaaagatc gtgccgaaaa tctgatgatt gtcgatttaa tgcgtaatga tatcggtcgt
     1741 gttgccgtag caggttcggt aaaagtacca gagctgttcg tggtggaacc cttccctgcc
     1801 gtgcatcatc tggtcagcac cataacggcg caactaccag aacagttaca cgccagcgat
     1861 ctgctgcgcg cggcttttcc tggtggctca ataaccgggg ctccgaaagt acgggctatg
     1921 gaaattatcg acgaactgga accgcagcga cgcaatgcct ggtgcggcag cattggctat
     1981 ttgagctttt gcggcaacat ggacaccagc attactatcc gcacgctgac tgccattaac
     2041 ggacaaatat actgctctgc gggcggagga attgttgccg atagccagga agaagcggaa
     2101 tatcaggaaa cttttgataa agttaataag atattacgcc aactggagaa gtaagacgtg
     2161 gaataccgta gcctgacgct tgatgatttt ttatcgcgct ttcaactttt gcgcccgcaa
     2221 attaaccggg aaaccttaaa tcatcgtcag gctgccgtgt taatccccat cgtccgccga
     2281 ccgcaaccgg ggttgttgct gactcagcgt tcgattcatc tgcgtaaaca cgctggacaa
     2341 gtggcattcc ctggaggcgc agtcgatgac acggacgcat cagttatcgc cgctgcgctg
     2401 cgcgaagctg aagaagaggt cgctataccg ccttccgccg ttgaggttat cggcgtgctg
     2461 ccgcccgtcg atagcgtcac tggctatcag gtaaccccag tggtcggcat tatcccgccc
     2521 gatctgccgt atcgcgccag tgaagatgaa gtctccgccg tctttgaaat gccgctcgcc
     2581 caggcattac atctgggtcg ttatcatcct ttagatatct accgccgtgg cgattcacat
     2641 cgggtatggc tgtcctggta cgaacagtat tttgtatggg gaatgaccgc aggcataatt
     2701 cgtgagctgg cgctgcaaat tggtgtgaaa ccctgactat acttatcttt acatctacaa
     2761 aacactactt gagacaatca tcgcaatatt agttaaatcg cggtttttga ttagtttaat
     2821 tcatgtgaat agttaagcct gtcgccgcgt tccctcttac actatgcgct gttattagtt
     2881 cgttactgga agtccagtca ccttgtcagg agtattatcg tgattagtct attcgacatg
     2941 tttaaggtgg ggattggtcc ctcatcttcc cataccgtag ggcctatgaa ggcgggtaaa
     3001 cagttcgtcg atgatctggt cgaaaaaggc ttactggata gcgttactcg cgttgccgtg
     3061 gacgtttatg gttcactgtc gctgacgggt aaaggccacc acaccgatat cgccattatt
     3121 atgggtcttg caggtaacga acctgccacc gtggatatcg acagtattcc cggttttatt
     3181 cgcgacgtag aagagcgcga acgtctgctg ctggcacagg gacggcatga agtggatttc
     3241 ccgcgcgaca acgggatgcg ttttcataac ggtaacctgc cgctgcatga aaacggtatg
     3301 caaatccacg cctataacgg cgatgaagtc gtctacagca aaacttatta ttccatcggc
     3361 ggcggtttta tcgtcgatga agaacacttt ggtcaggatg ctgccaacga agtgagcgtg
     3421 ccgtatccgt tcaaatctgc caccgaactg ctcgcgtact gtaatgaaac cggcttttcg
     3481 ctgtctggtc tcgctatgca aaacgaactg gcgctgcaca gcaagaaaga gatcgacgag
     3541 tatttcgcgc atgtctggca aaccatgcag gcatgtatcg atcgcgggat gaataccgaa
     3601 ggcgtactgc caggcccgtt gcgcgtgcca cgtcgtgcgt cggccctgcg ccggatgctg
     3661 gtttccagcg ataaactgtc taacgatccg atgaatgtca ttgactgggt aaacatgttt
     3721 gcgctggcag ttaacgaaga aaacgccgcc ggtggccgtg tggtaactgc gccaactaac
     3781 ggtgcctgcg gtatcgttcc ggcagtgctg gcttactatg accactttat tgaatcagtt
     3841 agcccagaca tctatacccg ttactttatg gcagcgggcg cgattggtgc actgtataaa
     3901 atgaacgcgt ctatttccgg tgcggaagtc ggttgccagg gcgaagtagg tgttgcctgt
     3961 tcaatggctg ctgcgggcct tgccgagctg ttgggcggta gcccggaaca ggtatgtgtg
     4021 gcagcggaaa ttggcatgga gcacaacctc ggtctgacct gcgaccccgt tgcagggcaa
     4081 gttcaggtgc cgtgcattga acgtaacgcc attgcctctg tgaaggcgat taacgccgcg
     4141 cggatggccc tgcgtcgcac cagtgcaccg cgcgtctcgc tggataaggt catcgaaacg
     4201 atgtacgaaa ccggtaagga catgaacgct aaataccgcg aaacctcacg cggtggtctg
     4261 gcaatcaaag tccagtgtga ctaatacttc ttattcgccc atctgcaacg gatgggcgaa
     4321 tttattcccg ctttctcgtc tgctgtaatt ttccccacta cacttccact gttgcgtcag
     4381 gcgtttgtcg ccatacgctt acagggtggc ccgcatgcaa aaagcacaac ggatcattaa
     4441 aacctatcgc cgtaatcgaa tgattgtttg tacgatttgc gcactcgtta cgctcgcatc
     4501 gaccctgagc gtgcgattta tttcacagcg taacttaaat caacaacggg tagtacaatt
     4561 cgccaatcac gctgtagagg aattagataa agtactgctt cccctacagg caggtagcga
     4621 agtcttgctt ccgctgattg gtctgccctg ctctgtcgcc catttgccat tacgtaaaca
     4681 ggcggcaaaa ctccaaactg tgcgctccat tggcctggtg caagacggca cactttattg
     4741 ctccagcatt tttggttatc gcaatgtgcc cgtcgtggac attctggctg aacttcctgc
     4801 accgcaacca cttttacgac tgacgaccga ccgtgccctg ataaaaggca gtccggtttt
     4861 gattcaatgg acgcctgcag cgggcagtag caatgctggg gtcatggaga tgattaacat
     4921 cgacttactg acggcaatgc tccttgaacc acaactgccg caaatcagta gcgccagcct
     4981 gacggtggac agtcggcatt tgctctatgg taatgggctg gtagattccc ttccgcaacc
     5041 tgaaaataat gaaaactatc aggtttcttc gcaacgcttt ccttttacca ttaacgttaa
     5101 tggtccgggg gctacggcgc tggcatggca ctatcttcca acacaattac cgctggcggt
     5161 gctgctaagt ttacttgtgg gctatatcgc ctggctggcg accgcttacc ggatgagttt
     5221 ttcccgcgaa atcaatctgg gcctggcgca acatgagttc gaattgttct gccagccttt
     5281 gcttaatgcg cgcagccagc aatgtattgg tgtagagatt ttgctgcgct ggaacaatcc
     5341 acgtcagggc tggatttcac cggatgtgtt tattcctatc gcggaagaac atcatttaat
     5401 tgtaccactg acccgctatg tgatggcaga aaccatccgt cagcggcatg ttttcccaat
     5461 gagtagtcag tttcatgttg gcattaacgt cgcacccagc cattttcgcc gtggtgtgct
     5521 gataaaagat ctcaatcagt actggtttag cgctcacccg attcagcaac tgatcctcga
     5581 aatcaccgaa cgcgatgcct tactggatgt tgattaccgg attgcccgcg agctgcatcg
     5641 taaaaacgtc aaactggcga ttgatgactt cggcaccggc aacagttcat tttcctggct
     5701 tgaaacatta cgtcctgacg tgctgaaaat tgataagtca tttaccgcag ctataggttc
     5761 tgatgcggtt aactcgacgg tgaccgatat catcatcgca ctggggcaaa gactgaatat
     5821 tgaactggtg gcggagggcg tagaaacgca agaacaggcg aagtatttgc gcagtcatgg
     5881 cgtgcatatt ttgcaagggt atttgtacgc acagccgatg ccgctacgtg attttcccaa
     5941 atggctggcg ggcagccaac cgccgcccgc ccggcataat ggacatatca cacccattat
     6001 gccgttacgt taacgcttac tcatcttcat cgtgcgccgg ttgctcttta acaatgcgaa
     6061 ccagatcaac acgataatca ttggcttcaa tgatggtgat atgcagtggc cctacatcaa
     6121 tcacatcgcc aacacgggga atgtgaccat ttgccgagat cacgaggccc gcgaccgtcg
     6181 cgatatcatc gtcatcggca aggtgctcaa catcaagcgc ctgctgcaag gcatgcaaat
     6241 ctgtaccgcc ttttaccagc cagccgtcac cgtcggtaat gatttccggc gtttcgtcag
     6301 cgtccgggaa ttcacccgca atggcttcca gcacatccag cggcgtgacc agaccttgta
     6361 ccacaccaaa ctcgttggtc acgataacaa aactcccgcg agcacgacgc agcacgccca
     6421 gcaggttgat cggatcgagg gtttccggga cgataatcgc cggagacgcc gaagctatcg
     6481 ccgccacatc aactccctct tccagcgcca ccagcagttc tttagcacgt acgatgccga
     6541 taatttcatc cagttcaccg cgacataccg ggaacaaact gtgtggtgag gagagcagtt
     6601 gctcgcggat ttcatcgacc ccgagattag catcaaccca gcttatttca ccgcgcggcg
     6661 tcatgatccc gcgcagagaa cgcgacgcca gcgtcagtac gccgttaatc atgtaacgtt
     6721 cttcttcggc aaacgcacct tccgggatcg gcatcggcat cgggttatcg gcatcgtgct
     6781 gaacattggc ctgacgtttc ccgcccatca aacggaggat ggcatcggca gtacgcgctc
     6841 gcagcggcaa agtcgactgg tgacgaataa agttgcgacg cgcaatctgg ttaaacactt
     6901 cgatgatgat cgagaagcca atcgcggcat acaggtaacc tttcggaatg tggaaaccga
     6961 aaccttctgc caccagactc agacctatca ttaacaggaa gcttagacag agcaccacca
     7021 ccgttggatg ctggttaaca aatcgcgtca gcggtttcga tgccagcagc ataaccgcca
     7081 tcgcaatcac taccgccgcc atcatcaccg gcaaatggtt aaccatccct actgcagtaa
     7141 ttaccgcatc caacgaaaag acggcgtcaa ggatgacaat ctgtgtgacg accacccaga
     7201 aactggcgta gcctttaccg tggccggaat catgatcgcg gttttccagc cgttcatgca
     7261 gttcggttgt tgctttgaac agcaagaata tgcccccgaa caacataatc aggtcgcgtc
     7321 cggagaagga gaaatccatg acggtaaata gcggtttggt cagcgtgacc atccatgaaa
     7381 tcagcgacag cagccccaga cgcataatca gcgccagtga taatcccagc aaacgcgctt
     7441 tatcgcgttg ttttggcggc agtttgtcag caagaatggc gatgaagacc aggttatcga
     7501 tacccagcac aatttcgaga acaacaagcg tgagtagccc cgcccaaatc gaggggtcca
     7561 ttaagaattc catgacaagc tcctgcttaa ggaatagcta ttcgacgcca gaaataatgc
     7621 aggcgtaacg acaaaatgca aacgaaaggt gcggcataaa gtgccagaaa gacaggcgtt
     7681 aaaaggcctg atgctgaaat gacgtcggtg acgatccata ctgcgggcta ctgccctata
     7741 ctccatggtt gttaaacagg agttaaacat atcagagacg cctctgattt ggcaaagatt
     7801 taccttcctt tgcaaacgaa tgtgacaagg atattttacc tttcgaaatt tctgctaatc
     7861 gaaagttaaa ttacggatct tcatcacata aaataatttt ttcgatatct aaaataaatc
     7921 gcgaaacgca gggggttttt ggttgtagcc cttatctgaa tcgattcgat tgtggacgac
     7981 gattcaaaaa tacatctggc acgttgaggt gttaacgata ataaaggagg tagcaagtga
     8041 ccattgctat tgttataggc acacatggtt gggctgcaga gcagttgctt aaaacggcag
     8101 aaatgctatt aggcgagcag gaaaacgtcg gctggatcga tttcgttcca ggtgaaaatg
     8161 ccgaaacgct gattgaaaag tacaacgctc agttggcaaa actcgacacc actaaaggcg
     8221 tgctgtttct cgttgataca tggggaggca gcccattcaa tgctgccagc cgcattgtcg
     8281 tcgacaaaga gcattatgaa gtcattgcag gcgttaacat tccaatgctc gtggaaacgt
     8341 taatggcccg agatgatgac cctagctttg atgaactggt ggcactggca gtagaaacag
     8401 gccgtgaagg cgtgaaagca ctgaaagcca aaccgattga aaaagccgcg ccagcacccg
     8461 ctgccgcagc acccaaagtg gctccaactc cggcaaaacc aatgggaccg aacgactaca
     8521 tggttattgg ccttgcgcgt atcgacgacc gtctgattca cggtcaggtc gccacccgct
     8581 ggaccaaaga aaccaatgtc tcccgtatta ttgttgttag tgatgaagtg gctgcggata
     8641 ccgttcgtaa gacactgctc acccaggttg cacctccggg cgtaacagca cacgtagttg
     8701 atgttgccaa aatgattcgc gtctacaaca acccgaaata tgctggcgaa cgcgtaatgc
     8761 tgttatttac caacccaaca gatgtagagc gtctcgttga aggcggcgtg aaaatcacct
     8821 ctgttaacgt cggtggtatg gcattccgtc agggtaaaac ccaggtgaat aacgcggttt
     8881 cggttgatga aaaagatatc gaggcgttca agaaactgaa tgcgcgcggt attgagctgg
     8941 aagtccgtaa ggtttccacc gatccgaaac tgaaaatgat ggatctgatc agcaaaatcg
     9001 ataagtaacg tattgtgttg attatcactc agttttcaca cttaagtctt acgtaaacag
     9061 gagaagtaca atggagatta ccactcttca aattgtgctg gtatttatcg tagcctgtat
     9121 cgcaggtatg ggatcaatcc tcgatgaatt tcagtttcac cgtccgctaa tcgcgtgtac
     9181 cctggtgggt atcgttcttg gcgatatgaa aaccggtatt attatcggtg gtacgctgga
     9241 aatgatcgcg ctgggctgga tgaacatcgg tgctgcagtt gcgcctgacg ccgctctggc
     9301 ttccatcatt tctactattc tggttatcgc aggtcatcag agcattggtg caggtatcgc
     9361 actggcaatc cctctggccg ctgcgggcca ggtactgacc atcatcgttc gaactatcac
     9421 cgttgctttc cagcacgctg cggataaggc tgctgataac ggcaacctga cagcgatttc
     9481 ctggatccac gtttcttctc tgttcctgca agcaatgcgt gtggctattc cggccgtcat
     9541 cgttgcgctg tctgttggta ccagcgaagt ccagaacatg ctgaatgcga ttccggaagt
     9601 ggtgaccaat ggtctgaata tcgccggtgg catgatcgtg gtggttggtt atgcgatggt
     9661 tatcaacatg atgcgtgctg gctacctgat gccgttcttc tacctcggct tcgtaaccgc
     9721 agcattcacc aactttaacc tggttgctct gggtgtgatt ggtactgtta tggcagtgct
     9781 ctacatccaa cttagcccga aatacaaccg cgtagccggt gcgcctgctc aggctgctgg
     9841 taacaacgat ctcgataacg aactggacta acaggtgagc gaaatggttg atacaactca
     9901 aactaccacc gagaaaaaac tcactcaaag tgatattcgt ggcgtcttcc tgcgttctaa
     9961 cctctttcag ggttcatgga acttcgaacg tatgcaggca ctgggtttct gcttctctat
    10021 ggtaccggca attcgtcgcc tctaccctga gaacaacgaa gctcgtaaac aagctattcg
    10081 ccgtcacctg gagttcttta acacccagcc attcgtggct gcgccgattc tcggcgtaac
    10141 cctggcactg gaagaacagc gtgctaatgg cgcagagatc gacgacggtg ctatcaacgg
    10201 catcaaagtc ggtttgatgg ggccgctggc tggtgtaggc gacccgatct tctggggaac
    10261 ggtacgtccg gtatttgcag cgctgggggc tggtatcgcg atgagcggca gcctgttagg
    10321 tccgctgctg ttcttcatcc tgtttaacct ggtgcgtctg gcaacccgtt actacggcgt
    10381 agcgtatggt tactccaaag gtatcgatat cgttaaagat atgggtggtg gcttcctgca
    10441 aaaactgacg gaaggggcgt ctatcctcgg cctgtttgtc atgggggcat tggttaataa
    10501 gtggacacat gtcaacatcc cgctggtggt ctctcgcatt actgaccaga cgggcaaaga
    10561 acacgttacc actgtccaga ctattctgga ccagttaatg ccaggcctgg taccactgct
    10621 gctgaccttt gcttgtatgt ggctactgcg caaaaaagtt aacccgctgt ggatcatcgt
    10681 tggcttcttc gtcatcggta tcgctggtta cgcttgcggc ctgctgggac tgtaagactg
    10741 ttgtacacta ccggggcctt ttggccccgt ttttttatct ggaggattaa tgacaatcac
    10801 ggacctggta ctgattcttt tcatcgccgc acttctggcc ttcgcgatct acgatcagtt
    10861 catcatgccc cgccgtaacg gccccaccct gctggcaatt cctttgctcc ggcgtggtcg
    10921 catcgatagc gttatcttcg tcggattgat tgtcattctt atctataaca acgtcacgaa
    10981 tcatggcgcg cttataacaa catggttatt aagtgcactg gctctgatgg gtttttatat
    11041 attctggatt cgcgttccga agatcatctt taaacaaaaa ggttttttct tcgccaatgt
    11101 ctggattgaa tatagccgaa tcaaagcgat gaacttgtcg gaagatggcg tgttggtgat
    11161 gcaattagaa cagcgtcggc tgttaatccg cgttcgaaat atcgacgatt tggaaaaaat
    11221 ttataagctt ctcgtttcaa ctcaataagt tatgaattta gccaaagcta tgtttagtgt
    11281 atttttaata atcatacata gcttaggcta tattagctct tcccttattt gttatttatt
    11341 ttaacgtttc attgatatat aaatctaaat gaaaatcgtt atcaataaaa caataa
//
LOCUS       DAHHTG010000129        11346 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.130, whole genome shotgun sequence.
ACCESSION   DAHHTG010000129 DAHHTG010000000
VERSION     DAHHTG010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11346)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11346)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11346
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.130"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            complement(<1..982)
                     /gene="pyrD"
                     /locus_tag="NBI74_003676"
     CDS             complement(<1..982)
                     /gene="pyrD"
                     /locus_tag="NBI74_003676"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HCD1933953.1"
                     /translation="MYYPFVRKALFQLDPERAHEFTFQQLRRITGTPFEALVRQKVPA
                     KPVNCMGLTFKNPLGLAAGLDKDGECIDALGAMGFGSIEIGTVTPRPQPGNDKPRLFR
                     LVDAEGLINRMGFNNLGVDNLVENVKKAHYDGVLGINIGKNKDTPVEQGKDDYLICME
                     KIYAYAGYIAINISSPNTPGLRTLQYGEALDDLLTAIKNKQNDLQVMHHKYVPIAVKI
                     APDLSEEELIQVADSLVRHNIDGVIATNTTLDRSLVQGMKNCDQTGGLSGRPLQLKST
                     EIIRRLSQELNGRLPIIGVGGIDSVIAAREKIAAGASLVQIYSGFIFKGPP"
     gene            1219..1794
                     /gene="ssuE"
                     /locus_tag="NBI74_003677"
     CDS             1219..1794
                     /gene="ssuE"
                     /locus_tag="NBI74_003677"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HCD1933954.1"
                     /translation="MRVITLAGSPRFPSRSSSLLEYAREKLNGLDVEVYHWNLQNFVP
                     EDLLYARFDSPALKTFTEQLQQADGLIVATPVYKAAYSGALKTLLDLLPERALQGKVV
                     LPLATGGTVAHLLAVDYALKPVLSTLKAQEILHGVFADDSQVIDYQHKPQFTPNLQTR
                     LDTALETFWQALHRRDVQVPDLLSLRGNAHA"
     gene            1787..2746
                     /gene="ssuA"
                     /locus_tag="NBI74_003678"
     CDS             1787..2746
                     /gene="ssuA"
                     /locus_tag="NBI74_003678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415456.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter
                     substrate-binding protein SsuA"
                     /protein_id="HCD1933955.1"
                     /translation="MRKIIKLVLAGLLSVSTIAVAAESSPEALRIGYQKGSIGMVLAK
                     SHQLLEKRYPQTKISWVEFPAGPQMLEALNVGSIDLGSTGDIPPIFAQAAGADLVYVG
                     VEPPKPKAEVILVAENSPIKTVADLKGHKVAFQKGSSSHNLLLRALRQAGLKFTDIQP
                     TYLTPADARAAFQQGNVDAWAIWDPYYSAALLQGGVRVLKDGTDLNQTGSFYLAARPY
                     AEKNGAFIQGVLTTFSEADALTRSQREQSIALLAKTMGLPAPVIASYLDHRPPTTIKP
                     LSAEVAALQQQTADLFYENRLVPKKVDIRQRIWQPTQLEGKQL"
     gene            2743..3888
                     /gene="ssuD"
                     /locus_tag="NBI74_003679"
     CDS             2743..3888
                     /gene="ssuD"
                     /locus_tag="NBI74_003679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMNH2-dependent alkanesulfonate monooxygenase"
                     /protein_id="HCD1933956.1"
                     /translation="MSLNMFWFLPTHGDGHYLGTEEGSRPVDHGYLQQIAQAADRLGY
                     TGVLIPTGRSCEDAWLVAASMIPVTQRLKFLVALRPSVTSPTVAARQAATLDRLSNGR
                     ALFNLVTGSDPQELAGDGVFLDHSERYEASAEFTQVWRRLLLGETVDFNGKHIHVRGA
                     KLLFPPIQQPYPPLYFGGSSDVAQELAAEQVDLYLTWGEPPKLVKEKIEHVRAKAAAH
                     GRKIRFGIRLHVIVRETNDEAWQAAERLISHLDDETIAKAQASFARTDSVGQQRMAAL
                     HNGKRDNLEISPNLWAGVGLVRGGAGTALVGDGPTVAARINEYAALGIDSFVLSGYPH
                     LEEAYRVGELLFPHLDVAIPEIPQPQPLNPQGEAVANDFIPRNVAQS"
     gene            3900..4691
                     /gene="ssuC"
                     /locus_tag="NBI74_003680"
     CDS             3900..4691
                     /gene="ssuC"
                     /locus_tag="NBI74_003680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415454.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter permease
                     SsuC"
                     /protein_id="HCD1933957.1"
                     /translation="MATPVKKWLLRVAPWFLPVGIVAVWQLASSVGWLSTRILPSPEG
                     VVMAFWTLSASGELWQHLAISSWRALIGFSIGGSLGLILGLISGLSRWGERLLDTSIQ
                     MLRNVPHLALIPLVILWFGIDESAKIFLVALGTLFPIYINTWHGIRNIDRELVEMARS
                     YGLSGMPLFIHVILPGALPSIMVGVRFALGLMWLTLIVAETISANSGIGYLAMNAREF
                     LQTDVVVVAIILYALLGKLADVSAQLLERLWLRWNPAYHLKEATV"
     gene            4688..5455
                     /gene="ssuB"
                     /locus_tag="NBI74_003681"
     CDS             4688..5455
                     /gene="ssuB"
                     /locus_tag="NBI74_003681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonates ABC transporter ATP-binding
                     protein"
                     /protein_id="HCD1933958.1"
                     /translation="MNTARLNQGTPLLLNAVSKHYAENIVLNQLDLHIPAGQFVAVVG
                     RSGGGKSTLLRLLAGLETPTAGDVLAGTTPLAEIQDDTRMMFQDARLLPWKSVIDNVG
                     LGLKGQWRDAARRALAAVGLENRAGEWPAALSGGQKQRVALARALIHRPGLLLLDEPL
                     GALDALTRLEMQDLIVSLWQEHGFTVLLVTHDVSEAVAMADRVLLIEEGKIGLDLTVD
                     IPRPRRLGSVRLAELEAEVLQRVMQRGEFETRLRKQG"
     gene            complement(5498..8110)
                     /gene="pepN"
                     /locus_tag="NBI74_003682"
     CDS             complement(5498..8110)
                     /gene="pepN"
                     /locus_tag="NBI74_003682"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000193787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HCD1933959.1"
                     /translation="MTQQPQAKYRHDYRAPDYQITDIDLTFDLDAQKTVVTAVSQAVR
                     HGASDVPLRLNGEDLKLVSVHINDEPWTAWKEEEGALVISNLPERFTLKIVNEISPAA
                     NTALEGLYQSGDALCTQCEAEGFRHITYYLDRPDVLARFTTKIIADKTKYPFLLSNGN
                     RVAQGELENGRHWVQWQDPFPKPCYLFALVAGDFDVLRDTFTTRSGREVALELYVDRG
                     NLDRAPWAMTSLKNSMKWDEERFGLEYDLDIYMIVAVDFFNMGAMENKGLNIFNSKYV
                     LARTDTATDKDYLDIERVIGHEYFHNWTGNRVTCRDWFQLSLKEGLTVFRDQEFSSDL
                     GSRAVNRINNVRTMRGLQFAEDASPMAHPIRPDMVIEMNNFYTLTVYEKGAEVIRMIH
                     TLLGEENFQKGMQLYFERHDGSAATCDDFVQAMEDASNVDLSHFRRWYSQSGTPVVTV
                     KDDYNPETEQYTLTISQRTPATPDQAEKQPLHIPFAIELYDNEGKVIPLQKGGHPVNS
                     VLNVTQAEQTFVFDNVYFQPVPALLCEFSAPVKLEYKWSDQQLTFLMRHARNDFSRWD
                     AAQSLLATYIKLNVARHQQGQPLSLPVHVADAFRAVLLDEKIDPALAAEILTLPSVNE
                     MAELFDIIDPIAIAEVREALTRTLATELADELLAIYNANYQSEYRVEHEDIAKRTLRN
                     ACLRFLAFGETHLADVLVSKQFHEANNMTDALAALSAAVAAQLPCRDALMQEYDDKWH
                     QDGLVMDKWFILQATSPAANVLETVRGLLQHRSFTMSNPNRIRSLIGAFAGSNPAAFH
                     AEDGSGYQFLVEMLTDLNSRNPQVASRLIEPLIRLKRYDAKRQEKMRAALEQLKGLEN
                     LSGDLYEKITKALA"
     gene            8376..9578
                     /gene="pncB"
                     /locus_tag="NBI74_003683"
     CDS             8376..9578
                     /gene="pncB"
                     /locus_tag="NBI74_003683"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HCD1933960.1"
                     /translation="MTQFASPVLHSLLDTDAYKLHMQQAVFHHYYDVHVAAEFRCRGD
                     DLLGIYADAIREQVQAMQHLRLQDDEYQWLSALPFFKADYLNWLREFRFNPEQVTVSN
                     DNGKLDIRLSGPWREVILWEVPLLAVISEMVHRYRSPQADVAQALDTLENKLVDFSAL
                     TAGLDMSRFHLMDFGTRRRFSREVQETIVKRLQQESWFVGTSNYDLARRLSLTPMGTQ
                     AHEWFQAHQQISPDLANSQRAALAAWLEEYPDQLGIALTDCITMDAFLRDFGVEFASR
                     YQGLRHDSGDPLEWGEKAIAHYEKLGIDPQSKTLVFSDNLDLRKAVELYRHFSSRVQL
                     SFGIGTRLTCDIPQVKPLNIVIKLVECNGKPVAKLSDSPGKTICHDKAFVRALRKAFD
                     LPHIKKAS"
     gene            9747..11147
                     /gene="asnS"
                     /locus_tag="NBI74_003684"
     CDS             9747..11147
                     /gene="asnS"
                     /locus_tag="NBI74_003684"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HCD1933961.1"
                     /translation="MSVVPVADVLQGRVAVDSEVTVRGWVRTRRDSKAGISFLAVYDG
                     SCFDPVQAVINNSLPNYNEDVLRLTTGCSVIVTGKVVASPGQGQQFEIQASKVEVAGW
                     VEDPNTYPMAAKRHSIEYLREVAHLRPRTNLIGAVARVRHTLAQALHRFFNEQGFFWV
                     STPLITASDTEGAGEMFRVSTLDLENLPRNDQGKVDFDKDFFGKESFLTVSGQLNGET
                     YACALSKIYTFGPTFRAENSNTSRHLAEFWMLEPEVAFANLNDIAGLAEAMLKYVFKA
                     VLEERADDMKFFAERVDKDAVSRLERFIEADFAQVDYTDAVTILENCGRKFENPVYWG
                     VDLSSEHERYLAEEHFKAPVVVKNYPKDIKAFYMRLNEDGKTVAAMDVLAPGIGEIIG
                     GSQREERLDVLDERMLEMGLNKEDYWWYRDLRRYGTVPHSGFGLGFERLIAYVTGVQN
                     VRDVIPFPRTPRNASF"
BASE COUNT         2606 a         2927 c         2987 g         2826 t
ORIGIN      
        1 gcggcggacc tttaaaaata aaaccagaat aaatttgcac cagtgaggca cccgcagcaa
       61 tcttttcacg cgcagcgata accgagtcaa tgccgccaac accaataatc ggcaagcgtc
      121 cgtttaattc ctgtgacaag cggcgaataa tttcggtgct ttttaactga agcggacgac
      181 cacttaagcc accggtttga tcacaatttt tcattccctg aaccagagaa cgatcgagtg
      241 tggtattggt tgcaataacg ccatcaatat tatggcgaac taaactatcg gcaacctgga
      301 tcaattcttc ttcagaaaga tccggcgcga tcttcactgc gatcggcaca tatttatggt
      361 gcatcacttg caaatcattt tgtttatttt taatcgcagt taagagatca tccagcgctt
      421 caccatattg cagtgtgcgt aatcctgggg tatttggcga tgaaatattg atggcgatat
      481 atcccgcata ggcatagatt ttttccatac aaatcagata gtcatcttta ccctgctcca
      541 ccggcgtatc tttatttttg ccgatgttaa tacccagaac gccgtcataa tgggcttttt
      601 ttacgttctc tacgaggtta tcaacgccaa gattattaaa gcccatacgg ttgatcaaac
      661 cttcggcatc taccagacga aagagacgcg gcttgtcatt acctggctgg ggacgtggcg
      721 tgacggtacc gatctcgatg gatccaaatc ccatcgcgcc taacgcgtca atgcactccc
      781 cgtctttatc aagaccggct gccagaccaa gcggattttt aaacgtcagg cccatgcagt
      841 taacaggttt cgcaggcact ttctgccgca ccagtgcttc aaacggcgtt cctgtaatac
      901 ggcgtaattg ctgaaaagta aactcatgag cgcgctctgg atcgagctgg aaaagggctt
      961 tacgaacgaa ggggtagtac atgaactctc ctggattccc ggtgtgcaaa ccgggggcgt
     1021 attatgtgcg agttcggacc aaaagggaat tgacctgcgg caataaatag caaacgtttt
     1081 cttattgcgc tttttttatg ctttttttgg cttttcttcc tcagataaat catttcgact
     1141 cgcggacgcc ctctgtcaga ctgaccgaat tgttatcaat gttaacaaaa caagaacaat
     1201 tggttataag gagagagtat gcgtgtcatc accctggcgg gtagtcctcg atttccttct
     1261 cgctccagct ccttgctgga atatgcgcgg gaaaaactaa atggcctgga tgtagaggtt
     1321 tatcactgga atttgcaaaa cttcgtcccg gaagatctgc tttatgctcg tttcgatagt
     1381 ccggcactca agaccttcac cgaacagctg caacaggccg atgggctgat tgtcgccacg
     1441 cctgtgtata aagccgccta ttccggtgca ctgaaaaccc tgctcgacct gctgccagaa
     1501 cgtgcgttgc aaggcaaagt ggtgctgccg ctggcgacag gcggcaccgt ggcccatctg
     1561 ctggcggtcg attatgccct taagccagtt ttaagtacgc tgaaagccca ggagatcctg
     1621 cacggcgtgt ttgccgatga ctcgcaagta attgattacc aacacaaacc ccagttcacg
     1681 ccaaatctgc aaactcgtct tgataccgcg ctagaaactt tctggcaggc gttgcaccgc
     1741 cgcgatgttc aggttcctga cctactgtct ttgcgaggta atgcccatgc gtaagatcat
     1801 taaactggtg ctggcgggtt tacttagcgt ctctacgatt gcggttgctg cagaatcctc
     1861 gcctgaagcg ttacgtatag gctatcaaaa aggcagtatt ggtatggtgc tggcaaaaag
     1921 ccaccagtta ctggaaaaac gctatccgca aacaaaaatc tcctgggtcg agttccccgc
     1981 tggcccacag atgctggagg cgttaaacgt tggcagtatc gatctcggca gtaccgggga
     2041 tattccgcca atctttgccc aggctgccgg ggctgatttg gtatacgtgg gcgtcgagcc
     2101 accgaagccc aaagccgaag tgattctggt ggcagaaaac agcccgatca aaaccgtagc
     2161 cgatcttaaa ggtcacaaag ttgcctttca gaaaggttcc agttcgcaca atcttttact
     2221 gcgagccctt cgccaggccg ggcttaaatt cacggatatt cagcccactt acctgacgcc
     2281 tgccgatgcc cgcgccgcgt tccagcaagg taacgttgac gcctgggcta tctgggatcc
     2341 ctactactcc gctgcattat tacagggcgg cgtgcgggtg ctgaaagacg gcaccgatct
     2401 caatcaaact ggatcgtttt atctggcagc tcgtccgtat gcagaaaaaa acggcgcttt
     2461 tattcagggt gtgctgacaa cctttagtga ggccgatgcg ttaacccgca gccagcgcga
     2521 acaaagcatc gctttactgg caaaaacgat gggcttaccg gcaccggtta ttgcctcgta
     2581 tctggatcat cgtcctccca ccaccatcaa accgttgagc gctgaagttg ccgccttaca
     2641 gcagcaaacg gcagatctgt tttatgaaaa ccgtctggtg ccgaaaaaag tcgatattcg
     2701 ccagcgcatc tggcagccca ctcaactgga aggaaaacaa ttatgagtct gaatatgttc
     2761 tggtttttac cgacccacgg tgacgggcat tatctgggaa cggaagaagg ttcacgtccg
     2821 gttgatcacg gttatctgca acaaattgcg caagcggcgg atcgtctggg ctataccggt
     2881 gtgctgatcc cgacggggcg atcgtgtgaa gatgcgtggc tggtggcggc gtcgatgatc
     2941 ccggtgacac aacggctgaa gtttcttgtc gccctgcgcc ccagcgtaac ctcacctacc
     3001 gttgccgccc gccaggccgc cacgcttgac cgtctctcta acggacgtgc gttgtttaac
     3061 ctggtcacag gcagcgatcc ccaagaactg gcaggcgacg gcgtgttcct tgatcatagc
     3121 gagcgttacg aagcctcggc agaatttacc caggtctggc ggcggttgtt gcttggcgag
     3181 actgtggatt tcaacggcaa acatattcat gtacgcgggg ccaaactgct cttcccgccg
     3241 attcaacagc cttatcctcc gctttacttt ggcggatcgt cagatgtcgc ccaggagctg
     3301 gcggcagaac aagttgatct ctaccttacc tggggcgaac caccgaaact ggtaaaagag
     3361 aaaatcgaac acgtgcgggc gaaagctgcc gcgcatggac gcaaaattcg tttcggtatt
     3421 cgtctgcatg tgattgttcg tgaaaccaac gacgaagcgt ggcaggccgc cgagcggtta
     3481 atctcgcatc ttgatgatga aactatcgcc aaagcacagg cctcattcgc ccgaacggat
     3541 tccgtaggac aacagcgaat ggcggcgcta cataacggca agcgcgacaa tctggagatc
     3601 agccccaatt tatgggcggg tgttggctta gtgcgcggcg gtgccgggac agcgctggtg
     3661 ggcgatggtc ctacggtcgc cgcgcgaatc aacgaatacg ctgcacttgg catcgacagt
     3721 tttgtgcttt cgggctaccc gcatctggaa gaagcgtatc gggtcggcga gttactgttc
     3781 ccacatctgg atgtcgccat cccggaaatt ccccagccac aaccgctgaa tccacaaggc
     3841 gaagcggtgg cgaatgattt tatcccccgt aacgtcgcgc aaagctaagg aacacgatga
     3901 tggcaacgcc agtgaagaag tggttattgc gcgttgcccc ctggttttta ccggtgggca
     3961 tcgtggcggt gtggcaactg gcctcctcgg ttggctggct atcgacgcgt attttgccct
     4021 caccggaagg agtggtgatg gcgttctgga cgctctctgc cagcggcgaa ctgtggcagc
     4081 atctggcgat cagctcctgg cgtgcactga ttggtttttc aattggcgga tcgctgggat
     4141 taattctggg gctgattagc gggctgtcac gctggggtga gcgactgctg gatacctcaa
     4201 ttcagatgtt gcgcaacgtg ccgcatctgg cgctgatccc gctggtgatt ttgtggtttg
     4261 gcattgatga atccgcaaaa atctttctgg tggcgctcgg tacgctattc cccatttata
     4321 tcaacacctg gcatggcatc cgtaatatcg accgtgagct ggtggagatg gcacgtagct
     4381 atggattatc cggcatgccg ctgtttatcc atgtgatcct gcctggtgcc ctgccctcaa
     4441 ttatggtcgg tgtgcgtttt gctttgggcc tgatgtggct gacgctgatc gttgccgaaa
     4501 ctatttctgc caattccggc attggttatc tggcgatgaa tgcgcgggag tttctgcaaa
     4561 cggacgtggt ggtagtcgcc attattcttt acgccctgct cggcaaactt gctgacgtca
     4621 gcgcgcagct gttagagcgt ctctggttgc gctggaaccc ggcttatcat ttgaaggagg
     4681 ccacggtatg aatactgctc gtctgaacca gggcacacca ttgttgctca atgcagtaag
     4741 caaacattac gcggaaaata tcgtcctgaa ccaactggat ttacatattc cagcaggtca
     4801 gtttgtggcg gtggtgggcc gcagcggtgg tggcaaaagt accctgctgc gcctgctggc
     4861 aggtctggaa acgccaaccg caggcgacgt gttagcgggc accacaccgc tggctgagat
     4921 tcaggatgat acgcgaatga tgtttcagga cgcgcgtctg ctgccgtgga aatcggtgat
     4981 tgataacgtt gggttaggcc ttaaaggcca gtggcgcgat gccgcgcgtc gggctctggc
     5041 tgcggtagga ctggagaatc gcgcggggga atggcctgcc gcactttctg gcgggcagaa
     5101 acaacgtgtg gcgctggcaa gggcgctgat tcatcgacca ggtttattgt tgcttgatga
     5161 accactcggt gcgctggacg ctttaacgcg actcgagatg caggatttga ttgtatcact
     5221 ctggcaggag cacggcttta ccgtcttgct ggtgacgcat gatgtgagcg aagcggtggc
     5281 gatggctgac cgggtgttgt taattgaaga gggaaaaatt ggtctggatt tgacggtgga
     5341 tatcccccgt ccgcgccggt tagggtcggt acgattagca gagctggaag cggaagtatt
     5401 gcagcgggtg atgcagcgtg gtgaatttga aactcgcctg agaaaacagg ggtaaattct
     5461 ccgaatggcg gcgctattgc cgccattcgg ttatttatca agccagtgct ttggtgatct
     5521 tctcgtacag atcgccagag agattttcca gccctttcaa ctgttccagc gccgcgcgca
     5581 ttttctcctg acgtttggca tcgtaacgtt tcaggcgaat caacggttca atcaggcgcg
     5641 aagccacttg tggatttcgg ctgttcaggt cggtaagcat ttccaccagg aactgataac
     5701 cgctgccatc ttcggcatgg aacgctgccg ggttgctgcc cgcaaatgcg ccaatcaatg
     5761 aacgaatgcg gttcgggttg ctcatggtaa atgagcgatg ctgcaacaga ccacgtaccg
     5821 tctccagcac attcgccgcc gggctggtgg cttgcaggat aaaccattta tccatcacca
     5881 gaccatcctg atgccacttg tcgtcgtact cctgcatcag cgcgtcacgg catggcagtt
     5941 gtgcagcaac cgccgcagaa agcgccgcca gcgcatcggt catattgttc gcttcgtgga
     6001 actgtttgct caccagcaca tcagccagat gcgtttcacc aaaagcgagg aagcgcaggc
     6061 aggcattacg cagagtgcgt tttgcaatat cttcatgctc aacacggtac tcgctctggt
     6121 aattcgcgtt gtaaatagcc agcagctcat ctgccagttc agtcgccaga gtacgagtga
     6181 gtgcttcgcg tacttcggca atagcaatcg gatcgatgat atcgaacagt tctgccattt
     6241 cattgacgga aggcagcgtc aggatttctg ccgccagcgc cggatcaatc ttctcatcga
     6301 gcagcaccgc gcggaaagcg tcagccacat gcaccggcag agacaacggc tgcccttgct
     6361 gatgacgggc gacgttcagc ttgatgtagg ttgccagcaa actttgcgcc gcgtcccagc
     6421 gagagaaatc attacgcgca tgacgcatca ggaaggtcag ttgctgatcg ctccacttat
     6481 attccagttt cactggcgca gagaattcgc acagcagcgc aggcaccggc tggaagtaga
     6541 cattatcaaa aacaaaggtc tgttccgcct gggtgacgtt cagcacggaa ttcaccggat
     6601 gaccgccttt ctgcaacggg atcactttgc cttcgttgtc atacagttcg atggcaaacg
     6661 gaatatgcag cggttgtttt tctgcctgat ccggcgtagc tggcgtgcgc tggctgatgg
     6721 tcagggtgta ctgctcagtt tccggattgt agtcgtcttt gacggtcaca accggcgtgc
     6781 cggactggct gtaccaacgg cggaaatggg agagatcgac attcgacgca tcttccatcg
     6841 cctgcacaaa atcgtcacag gtcgctgcgc taccatcatg acgctcgaaa tagagctgca
     6901 tccctttctg gaaattttct tcgccaagca gagtgtggat catacgaatc acttccgcgc
     6961 ccttctcgta aacggtcagg gtgtagaagt tgttcatttc aatgaccata tccgggcgaa
     7021 ttgggtgcgc catcgggctg gcgtcttctg caaactgcaa tccgcgcatg gtgcgcacat
     7081 tattgatacg gtttaccgcg cgggaaccaa ggtcagagct gaattcctga tcgcggaaga
     7141 cggttaaacc ttctttcaga ctgagctgga accagtcgcg gcaggtgaca cggttgccgg
     7201 tccagttatg gaaatattca tggccgataa cgcgttcaat atcgaggtaa tctttgtcgg
     7261 tggcggtgtc ggtgcgggcc agcacatatt tggagttaaa gatattcagc cccttattct
     7321 ccattgcgcc catattgaag aaatccaccg cgacgatcat atagatgtcg aggtcatact
     7381 ccaggccgaa gcgttcttca tcccatttca tggagttttt cagcgaggtc attgcccacg
     7441 gcgcacgatc aaggttgcca cgatcgacat acaactccag cgccacttca cgaccagagc
     7501 gggtagtgaa ggtatcgcgc agtacatcaa aatcaccagc caccagcgca aacaggtaac
     7561 acggtttcgg gaatggatcc tgccactgta cccaatgacg tccattttcc agttcacctt
     7621 gcgcaacgcg gttaccgttg gaaagcagga agggatattt ggttttatcg gcaataattt
     7681 tggtggtaaa acgcgccagc acgtccgggc ggtcgagata atacgtaata tggcggaaac
     7741 cttcggcttc acactgggtg caaagcgcat cgcctgactg ataaagccct tccagcgcag
     7801 tattcgccgc cgggctaatt tcattaacga tcttaagcgt aaaacgctcc ggcaaattac
     7861 tgatgaccag tgcgccctct tcttctttcc aggcggtcca cggctcatca ttaatatgaa
     7921 cagaaaccag tttgaggtct tcgccgttga gacgaagcgg aacatctgat gcaccatgac
     7981 ggacagcctg gctgaccgcg gtaacgaccg tcttttgcgc gtccaggtca aaggtcaagt
     8041 caatatcagt aatctggtaa tccggcgcac gataatcgtg acggtatttg gcttgtggct
     8101 gttgagtcat aaataacctt tagcatcttt tatagagtct ggtgttcagt ctattcctgt
     8161 tgcgtaaatc gcgctatgca gaatcttcat cttttcaggt acaaacgcct ttattgctac
     8221 atttttataa catacaccgc gttatgccat cgaccagaaa ggtggcatat ggtgtgatcg
     8281 gggttcaata aattgcgaaa caaggtatac tccagcagtt cctgaagatg tttattgtac
     8341 taaacgctcc tgtacgagga cgctactgcg cacctatgac acaattcgct tctcctgttc
     8401 tgcactcgtt gctggataca gatgcttata agttgcatat gcagcaagcc gtgtttcatc
     8461 actattacga tgtgcatgtc gcggcggagt ttcgttgccg aggtgacgat ctgctgggta
     8521 tttatgccga tgctattcgt gaacaggttc aggcgatgca gcacctgcgc ctgcaggatg
     8581 atgaatatca gtggctctct gccctgcctt tctttaaggc cgactatctt aactggctac
     8641 gcgagttccg ctttaacccg gaacaagtca ccgtgtccaa cgataatggc aagctggata
     8701 ttcgtttaag cggcccgtgg cgtgaagtca tcctctggga agttcctttg ctggcggtta
     8761 tcagtgaaat ggtacatcgc tatcgctcac cgcaggccga cgttgcgcaa gccctcgaca
     8821 cgctggaaaa caaattagtc gacttctcgg cattaaccgc cggtcttgat atgtcgcgct
     8881 tccatctgat ggattttggc acccgccgcc gtttttctcg cgaagtacaa gaaaccatcg
     8941 tgaaacgtct gcaacaggaa tcctggttcg tgggcaccag caactacgat ctggcgcgtc
     9001 ggctttccct cacgccaatg ggaacacagg cacacgaatg gttccaggcg catcagcaaa
     9061 tcagcccgga tctagccaac agccagcgag ctgcacttgc tgcctggctg gaagagtatc
     9121 ccgaccaact tggcattgcg ttaaccgact gcatcaccat ggatgctttc ctgcgagatt
     9181 tcggcgtgga atttgccagt cgttaccagg gcctgcgcca tgactctggc gacccgcttg
     9241 aatggggtga aaaagccatt gcacattatg aaaagctggg aattgatcca cagagtaaaa
     9301 cgctggtttt ctctgacaat ctggatttac gcaaagcggt tgagctatac cgccacttct
     9361 cttcccgcgt gcaattaagt tttggtattg ggactcgcct gacctgcgat atcccccagg
     9421 taaaacctct gaatattgtc attaagttgg tagagtgtaa cggtaaaccg gtggcgaaac
     9481 tttctgacag ccctggcaaa acgatctgcc acgataaagc gtttgttcgg gcgctgcgca
     9541 aagcgttcga ccttccgcat attaaaaaag ccagttaata tcatcaggga gctaatcggc
     9601 tccctttttt tacctttaat tccgaaatct ttcgctgcat ttgcgaattc tgcttgtctg
     9661 attgcagatg ccaggtaaca taggtatccc cccatttacg ggtggacaag tgtttatttt
     9721 ttccgactat taacagagag aatattatga gcgttgtgcc tgtagccgac gtactccagg
     9781 gccgcgtagc cgttgacagc gaagtcaccg tgcgcggatg ggtacgtacc cgccgagatt
     9841 caaaagctgg catctccttc ctcgccgttt atgacggttc ctgctttgat cctgtacagg
     9901 ctgtcatcaa taattctctg cccaattaca atgaagacgt cctgcgtctg accaccggct
     9961 gctcggtcat tgtaacgggt aaagtcgtgg cgtcgccggg ccaggggcaa caatttgaaa
    10021 ttcaggccag caaggttgaa gttgctggtt gggttgaaga tccaaacact tacccgatgg
    10081 cggcaaaacg ccacagcatt gagtatctgc gtgaagtcgc tcacctgcgt ccgcgcacaa
    10141 acctgattgg tgccgtcgcg cgcgttcgcc atacgctggc gcaggcgctg catcgcttct
    10201 ttaacgagca gggattcttc tgggtttcaa cgccactgat taccgcatct gataccgaag
    10261 gtgcaggcga aatgttccgt gtttctacgc tggatctgga aaacctgccg cgtaacgatc
    10321 agggcaaagt ggatttcgac aaagacttct ttggtaaaga gtctttcctg accgtatctg
    10381 gccagttgaa cggcgaaacc tacgcttgcg cattgtccaa aatttatacc ttcggcccga
    10441 ctttccgtgc tgaaaactcc aacaccagcc gtcacctggc ggaattctgg atgctggagc
    10501 cggaagtggc gtttgctaac ctgaacgata tcgcaggtct ggctgaagcc atgctgaaat
    10561 atgtcttcaa agcggttctc gaagaacgcg ctgacgacat gaaattcttc gctgaacgcg
    10621 tagataaaga tgccgtttca cgtctggaac gcttcattga ggccgatttc gcgcaggtgg
    10681 attacaccga cgcagtgacc attctcgaaa actgcggcag gaagtttgaa aacccggttt
    10741 actggggcgt cgatctctct tctgagcatg agcgttatct ggcggaagaa cactttaaag
    10801 caccagtagt ggttaaaaac tatccgaaag atattaaagc gttctatatg cgccttaacg
    10861 aagacggtaa aaccgttgcg gctatggacg ttctggctcc gggcatcggt gagatcattg
    10921 gtggctccca gcgtgaagaa cgtctggacg tgctggacga gcgtatgctt gaaatgggcc
    10981 tgaacaaaga agattactgg tggtatcgcg atctgcgtcg ctacggtact gttccgcatt
    11041 caggtttcgg tcttggtttt gaacgcctga ttgcttacgt aactggcgtg caaaacgtac
    11101 gtgatgttat tccgttccca cgtactccgc gtaacgccag cttctaattt agtgtcttca
    11161 agagccagcg cccgctggct ctttttttat cctttctgtc aagttatctg tttgttaagt
    11221 caagcaatct atttgcaacc ctgccataac gatcatcctg ttacggaata ttacactgca
    11281 acatttacgc acaaaaaata atctgcattc ttattgcgga ttagtttttt cttagctgat
    11341 agcaca
//
LOCUS       DAHHTG010000130        11166 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.131, whole genome shotgun sequence.
ACCESSION   DAHHTG010000130 DAHHTG010000000
VERSION     DAHHTG010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11166)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11166)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11166
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.131"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            8..112
                     /locus_tag="NBI74_003685"
     CDS             8..112
                     /locus_tag="NBI74_003685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001312672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2618 domain-containing protein"
                     /protein_id="HCD1933962.1"
                     /translation="MENNNRLMPHIRRTTHIMKFAHRNSFDFHFFNAR"
     gene            422..2620
                     /gene="speF"
                     /locus_tag="NBI74_003686"
     CDS             422..2620
                     /gene="speF"
                     /locus_tag="NBI74_003686"
                     /EC_number="4.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase SpeF"
                     /protein_id="HCD1933963.1"
                     /translation="MSELKIAVSRSCPDCFSTHRECVNIDKSNYIDVAAIILSVNDVE
                     HGKLDEIDATGYGIPVFIATENEERVPAEYLPRISGVFEHCESRKEFYGRQLETAASH
                     YETQLRPPFFRALVDYVNQGNSAFDCPGHQGGEFFRRHPAGNQFVEYFGEMLFRSDLC
                     NADVAMGDLLIHEGAPCIAQQHAAKVFNADKTYFVLNGTSSSNKVVLNALLTPGDLVL
                     FDRNNHKSNHHGALLQAGATPVYLETARNPYGFIGGIDAHCFEESYLRELITEVAPQR
                     AKEARPFRLAVIQLGTYDGTIYNARQVVDKIGHLCDYILFDSAWVGYEQFIPMMADCS
                     PLLLELNENDPGILVTQSVHKQQAGFSQTSQIHKKDSHIKGQQRYVPHKRMNNAFMMH
                     ASTSPFYPLFAALDINAKMHEGVSGRNMWMDCVVNGINARKLILDNCQHIRPFVPELV
                     DGKPWQSYETAQIAVDLRFFKFVPGEHWHSFEGYAENQYFVDPCKLLLTTPGIDARNG
                     EYEAFGVPATILANFLRENGVVPEKCDLNSILFLLTPAEDMAKLQQLVALLVRFEKLL
                     EADAPLAEVLPSIYKQHEERYAGYTLRQLCQEMHDLYARHNVKQLQKEMFRKEHFPRV
                     SMNPQEANYAYLRGEVELVRLPDAEGRIAAEGALPYPPGVLCVVPGEIWGGAVLRYFS
                     ALEEGINLLPGFAPELQGVYIEEHDGRKQVWCYVIKPRDAQSALLKGEKL"
     gene            2617..3936
                     /gene="potE"
                     /locus_tag="NBI74_003687"
     CDS             2617..3936
                     /gene="potE"
                     /locus_tag="NBI74_003687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine-ornithine antiporter"
                     /protein_id="HCD1933964.1"
                     /translation="MSQAKSNKMGVVQLTILTMVNMMGSGIIMLPTKLAEVGTISIIS
                     WLVTAVGSMALAWAFAKCGMFSRKSGGMGGYAEYAFGKSGNFMANYTYGVSLLIANVA
                     IAISAVGYGTELLGASLSPVQIGLATIGVLWICTVANFGGARITGQISSITVWGVIIP
                     VVGLCIIGWFWFSPTLYVDSWNPHHAPFFSAVGSSIAMTLWAFLGLESACANTDVVEN
                     PERNVPIAVLGGTLGAAVIYIVSTNVIAGIVPNMELANSTAPFGLAFAQMFTPEVGKV
                     IMALMVMSCCGSLLGWQFTIAQVFKSSSDEGYFPKIFSRVTKVDAPVQGMLTIVIIQS
                     GLALMTISPSLNSQFNVLVNLAVVTNIIPYILSMAALVIIQKVANVPPSKAKVANFVA
                     FVGAMYSFYALYSSGEEAMLYGSIVTFLGWTLYGLVSPRFELKNKHG"
     gene            complement(3993..5633)
                     /gene="pgm"
                     /locus_tag="NBI74_003688"
     CDS             complement(3993..5633)
                     /gene="pgm"
                     /locus_tag="NBI74_003688"
                     /EC_number="5.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-glucose phosphate-specific
                     phosphoglucomutase"
                     /protein_id="HCD1933965.1"
                     /translation="MAIHNRAGQPAQQSDLINVAQLTAQYYVLKPEAGNAEHAVKFGT
                     SGHRGSAARHSFNEPHILAIAQAIAEERAKNGITGPCYVGKDTHALSEPAFISVLEVL
                     AANGVDVIVQENNGFTPTPAISNAILVHNKKGGPLADGIVITPSHNPPEDGGIKYNPP
                     NGGPADTNVTKVVEDRANALLADGLKGVKRISLDEAMASGHVKEQDLVQPFVEGLADI
                     VDMAAIQKAGLTLGVDPLGGSGIEYWKRIGEYYNLNLTIVNDQVDQTFRFMHLDKDGA
                     IRMDCSSECAMAGLLALRDKFDLAFANDPDYDRHGIVTPAGLMNPNHYLAVAINYLFQ
                     HRPQWGKDVAVGKTLVSSAMIDRVVNDLGRKLVEVPVGFKWFVDGLFDGSFGFGGEES
                     AGASFLRFDGTPWSTDKDGIIMCLLAAEITAVTGKNPQEHYNELAKRFGVPSYNRLQA
                     AATSAQKAALSKLSPEMVSASTLAGDPITARLTAAPGNGASIGGLKVMTDNGWFAARP
                     SGTEDAYKIYCESFLGEEHRKQIEKEAVEIVSEVLKNA"
     gene            complement(5659..6204)
                     /gene="seqA"
                     /locus_tag="NBI74_003689"
     CDS             complement(5659..6204)
                     /gene="seqA"
                     /locus_tag="NBI74_003689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation negative regulator SeqA"
                     /protein_id="HCD1933966.1"
                     /translation="MKTIEVDDELYSYIASHTKHIGESASDILRRMLKFTAASQPAAP
                     VTKEVRVASPAIVEAKPVKTIKDKVRAMRELLLSDEYAEQKRAVNRFMLLLSTLYSLD
                     AQAFAEATESLHGRTRVYFAADEQTLLKNGNQTKPKHVPGTPYWVITNTNTGRKCSMI
                     EHIMQSMQFPAELIEKVCGTI"
     gene            6389..7153
                     /gene="ybfF"
                     /locus_tag="NBI74_003690"
     CDS             6389..7153
                     /gene="ybfF"
                     /locus_tag="NBI74_003690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HCD1933967.1"
                     /translation="MKLNIRAQTAQNQHNNSPIVLVHGLFGSLDNLGVLARDLVNDHN
                     IIQVDMRNHGLSPRDPVMNYPAMAQDLVDTLDAQQIDKATFIGHSMGGKAVMALTALA
                     PDRIDKLVAIDIAPVDYHVRRHDEIFAAINAVSESDAQTRQQAAAIMRQHLNEEGVIQ
                     FLLKSFVDGEWRFNVPVLWDQYPHIVGWEKIPAWDHPTLFIPGGNSPYVSEQYRDDLL
                     AQFPQARAHVIAGAGHWVHAEKPDAVLRAIRRYLND"
     gene            7293..7586
                     /gene="ybfE"
                     /locus_tag="NBI74_003691"
     CDS             7293..7586
                     /gene="ybfE"
                     /locus_tag="NBI74_003691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA regulated protein"
                     /protein_id="HCD1933968.1"
                     /translation="MAKEQTDRTTLDLFAHERRPGRPKTNPLSRDEQLRINKRNQLKR
                     DKVRGLKRVELKLNAEAVEALNELAESRNMSRSELIEEMLMQQLAALRSQGIV"
     gene            7726..8256
                     /gene="fldA"
                     /locus_tag="NBI74_003692"
     CDS             7726..8256
                     /gene="fldA"
                     /locus_tag="NBI74_003692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldA"
                     /protein_id="HCD1933969.1"
                     /translation="MAITGIFFGSDTGNTENIAKMIQKQLGKDVADVHDIAKSSKEDL
                     EAYDILLLGIPTWYYGEAQCDWDDFFPTLEEIDFNGKLVALFGCGDQEDYAEYFCDAL
                     GTIRDIIEPRGATIVGHWPTAGYHFEASKGLADDDHFVGLAIDEDRQPELTAERVEKW
                     VKQISEELHLDEILNA"
     gene            8466..8552
                     /gene="uof"
                     /locus_tag="NBI74_003693"
     CDS             8466..8552
                     /gene="uof"
                     /locus_tag="NBI74_003693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001491546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fur leader peptide"
                     /protein_id="HCD1933970.1"
                     /translation="MIRIISRANSVTSSNEVNRLVTGQIPHD"
     gene            8545..8991
                     /gene="fur"
                     /locus_tag="NBI74_003694"
     CDS             8545..8991
                     /gene="fur"
                     /locus_tag="NBI74_003694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HCD1933971.1"
                     /translation="MTDNNTALKKAGLKVTLPRLKILEVLQEPDNHHVSAEDLYKRLI
                     DMGEEIGLATVYRVLNQFDDAGIVTRHNFEGGKSVFELTQQHHHDHLICLDCGKVIEF
                     SDDSIEARQREIAAKHGIRLTNHSLYLYGHCAEGDCREDEHAHEGK"
     gene            complement(9075..9401)
                     /locus_tag="NBI74_003695"
     CDS             complement(9075..9401)
                     /locus_tag="NBI74_003695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1933972.1"
                     /translation="MKKLILIAMMASGLVACAQSTAPQEDSRLKEAYSACINTAQGSP
                     EKIEACQSVLNVLKKEKQHQQFAEQESVRVLDYQQCIQATQTGNDQAVKADCDKVWQE
                     IRSNNK"
     gene            complement(9451..10857)
                     /gene="chiP"
                     /locus_tag="NBI74_003696"
     CDS             complement(9451..10857)
                     /gene="chiP"
                     /locus_tag="NBI74_003696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitoporin"
                     /protein_id="HCD1933973.1"
                     /translation="MRAFSGKRSTLALAIAGVTAMSGFMAIPEARAEGFIDDSTLTGG
                     IYYWQRERDRKDVTDGDKYKTNLSHSTWNANLDFQSGYAADMFGLDIAAFTAIEMAEN
                     GDSSHPNEIAFSKSNKAYDEDWSGDKSGISLYKAAAKFKYGPVWARAGYIQPTGQTLL
                     APHWSFMPGTYQGAEAGANFDYGDAGALSFSYMWTNEYKAPWHLEMDEFYQNDKTTKV
                     DYLHSLGAKYDFKNNFVLEAAFGQAEGYIDQYFAKASYKFDIAGSPLTTSYQFYGTRD
                     KVDDRSANDLYDGTAWLQALTFGYRAADVVDLRLEGTWVKADGQQGYFLQRMTPTYAS
                     SNGRLDIWWDNRSDFNANGEKAVFFGAMYDLKNWNLPGFAIGASYVYAWDAKPATWQS
                     NPDAYYDKNRTIEESAYSLDAVYTIQDGRAKGTMFKLHFTEYDNHSDIPSWGGGYGNI
                     FQDERDVKFMVIAPFTIF"
BASE COUNT         2724 a         2769 c         2766 g         2907 t
ORIGIN      
        1 aaggtaaatg gaaaataata accgcttaat gcctcatata aggcggacaa cccatatcat
       61 gaagtttgcc catcgtaata gttttgactt tcatttcttt aatgcccgct agtcttctga
      121 ctaaagggca ccccagcgta caggtctccc tgactttaag cattacaggt taatacctgt
      181 atccctcggt gctcatatac tgctaaccct tttaaactct aaataattcg agtcgcagca
      241 catgcaactt gaagtatgac gagtatagcc agttaccggg ctggtctggg ttattgcatc
      301 tgcaaaaagc aaactactga tttatttatc agcggtgggg ttttgctttt ttccggcgtg
      361 atcaatttct cctttgagaa attgaggacc tgctattacc tgaaataaag agatgaacaa
      421 aatgtcagaa ttaaaaattg ccgttagccg ttcttgcccg gattgttttt ccactcatcg
      481 tgaatgcgtg aatattgaca aaagtaatta tattgacgtt gccgccatta ttttatcagt
      541 caatgatgtt gaacatggaa aactcgatga aatagacgct actggctatg gcattcctgt
      601 ttttattgca acggaaaatg aagaacgtgt accagcagaa tatctgccac gtatttctgg
      661 tgtcttcgag cattgcgaat cacggaaaga gttttatgga cgtcagttag aaaccgctgc
      721 cagccattat gaaacgcaac tgcgcccgcc cttcttccgc gcactggtcg attatgtcaa
      781 tcagggcaat agcgcttttg actgcccggg tcatcaggga ggtgagtttt tccgtcgcca
      841 tccggcaggc aatcagttcg tggaatattt tggcgaaatg ctcttccgct ccgacttgtg
      901 taatgccgac gttgcaatgg gcgatctact gattcatgaa ggtgcgccat gcattgcaca
      961 gcaacatgcg gctaaagtgt ttaatgccga taaaacgtac tttgttttaa acggcacatc
     1021 ctcctccaac aaagtggttt taaacgccct gctgacaccg ggtgatctgg tgctgtttga
     1081 ccgtaataac cataagtcta accaccacgg agcgttgcta caggctggtg caacaccggt
     1141 ttatctggaa acagcacgta acccgtatgg ttttatcggt ggcattgatg cgcactgctt
     1201 cgaagaaagt tatctgcgtg aactgattac agaagttgca ccgcaacggg caaaagaggc
     1261 gcgtccgttc cgcctcgctg tgattcagtt aggcacctac gacggtacta tttataacgc
     1321 ccgccaggtg gtggataaaa tcggccatct gtgtgactac atcctgtttg actccgcatg
     1381 ggtcggctat gaacagttta ttccgatgat ggcggactgt tcgccgctgc tactggagct
     1441 taatgagaac gatccgggta ttctggttac gcaatctgtg cataaacaac aggctggttt
     1501 ttctcagact tcacaaattc ataaaaaaga cagccatatc aaagggcaac agcgttatgt
     1561 accgcacaaa cgcatgaaca acgcctttat gatgcacgcc tccaccagcc cgttctatcc
     1621 gctgtttgcc gcactggata tcaacgccaa aatgcatgaa ggtgtcagcg gtcgtaatat
     1681 gtggatggat tgtgtggtta acggtattaa tgcccgcaaa ttgatcctcg ataactgcca
     1741 gcacattcgc ccgttcgttc ctgaactggt ggatggtaaa ccctggcagt cgtatgaaac
     1801 agcgcaaatt gcggttgatc tgcgcttctt taaatttgta cccggtgagc actggcattc
     1861 ttttgaaggc tacgcagaga accaatactt tgtcgatccg tgcaaactgt tgctgacaac
     1921 tccaggtatt gatgcacgta acggcgaata tgaagcgttc ggtgtacccg caacgattct
     1981 tgctaacttc ctgcgcgaaa atggcgtagt gccggaaaaa tgcgatctta actccatcct
     2041 cttcctgctg actccggcag aagatatggc caaactgcag caacttgttg ccctgctggt
     2101 acgcttcgaa aaactactgg aagctgatgc gcctctggcg gaagtactgc cttccatcta
     2161 caaacagcat gaagagcgct acgccggtta taccctgcgt cagttgtgcc aggaaatgca
     2221 tgatttgtat gcccgtcaca acgtgaaaca actgcaaaaa gagatgttcc gtaaggaaca
     2281 cttccctcgc gtcagcatga atccgcaaga agccaactac gcctacttac gcggtgaagt
     2341 ggagctggtt cgtctgccgg atgcagaagg ccgtatcgct gccgaaggtg cgcttcctta
     2401 tcctccgggt gtgctgtgtg ttgttcctgg tgaaatctgg ggtggtgctg ttctgcgtta
     2461 cttcagcgct ctggaagaag gcatcaacct gctgccaggt tttgcaccgg agctgcaggg
     2521 tgtctatatc gaagaacatg atggtcgtaa gcaagtttgg tgctatgtca tcaagcctcg
     2581 tgatgcgcaa agcgccctgt tgaaagggga aaaattatga gtcaggcgaa atcgaacaaa
     2641 atgggtgtcg ttcagttaac catactgacg atggtcaaca tgatgggctc cggtatcatc
     2701 atgctgccga caaagcttgc cgaagtcggg acaatctcaa ttatctcctg gctggtgaca
     2761 gccgtaggct caatggcact ggcatgggca ttcgcaaaat gcggtatgtt cagccgtaaa
     2821 tcaggaggta tgggcggtta tgccgagtat gctttcggta aatccggtaa ctttatggcg
     2881 aactatacct acggcgtctc gctgctgatt gctaacgtcg cgattgctat ttcggcggtt
     2941 ggttacggca ccgaactgct cggcgcgagt ttgtcgccag tgcagattgg tcttgcgacc
     3001 atcggggtgc tgtggatttg taccgtggct aactttggtg gtgcgcgcat taccgggcaa
     3061 atcagtagca ttaccgtgtg gggggtcatt attccggtgg taggcctgtg cattatcggc
     3121 tggttctggt ttagcccgac gctttacgtt gattcctgga atccgcatca tgcacccttc
     3181 ttcagtgcgg taggttcttc catcgctatg acgctgtggg cttttcttgg tctggagtct
     3241 gcgtgtgcga atactgatgt agtggaaaac ccggaacgta atgtgccaat cgcggtactc
     3301 ggcggtacgt taggtgcggc ggtgatttat atcgtctcca ccaacgtgat tgccgggatt
     3361 gtgccaaata tggagctggc aaattcaacg gcaccatttg gtctggcctt cgcgcagatg
     3421 ttcacgccgg aagtgggtaa agtcattatg gcgctgatgg tgatgtcctg ctgcggttcg
     3481 ctgcttggct ggcagttcac cattgcccag gtgtttaaat cttcatctga tgaaggctac
     3541 ttccctaaaa ttttctcccg tgtaaccaaa gtggatgcac cggtgcaggg aatgttgacc
     3601 attgtgatta tccagagcgg gttggcgctg atgacgatta gcccgtcgct aaacagtcag
     3661 ttcaacgtgc tggttaacct ggccgtggtg actaatatca ttccgtatat tctgtcaatg
     3721 gcggcattag tcattattca gaaggtcgct aatgtgcccc cctcaaaagc gaaagttgca
     3781 aactttgttg cttttgttgg cgcgatgtac agcttttatg cgctgtactc atccggggaa
     3841 gaagccatgc tatacggttc catcgtgacc ttcctcggtt ggacactgta tggtctggtc
     3901 tcaccacgct ttgaactgaa aaataaacac ggttaattaa gcaaagcaaa aaagggcgat
     3961 cttccgaccg ccctttttct tatttgatat gtttacgcgt ttttcagaac ttcgctaaca
     4021 atttcaaccg cttctttctc aatctgcttg cgatgttctt caccaaggaa gctttcgcag
     4081 tagatcttat atgcgtcttc cgtgcctgac ggacgcgcgg cgaaccagcc gttgtcagtc
     4141 atcactttca gaccgccgat agaagcgcca ttacccggtg ccgccgtcag acgcgcggtg
     4201 atcgggtcac ctgccagggt gctggcgctc accatttccg gagacagctt agacaacgcc
     4261 gctttttgtg cggaagtcgc tgctgcctgc aaacggttat agctcggcac accaaagcgt
     4321 tttgccagtt cgttatagtg ttcctgcggg ttcttaccgg tgacagcggt gatttccgcc
     4381 gccagcagac acataatgat gccgtcttta tcggtggacc acggcgtgcc gtcgaaacgc
     4441 aggaaggaag cccctgcgct ctcttcgccg ccaaagccga agctgccgtc gaacagacca
     4501 tcaacaaacc atttgaaacc taccgggact tctaccagct tacggcccaa gtcattgacc
     4561 acacggtcga tcatcgcaga tgaaaccagc gttttaccga cagcaacatc tttgccccac
     4621 tgcggacgat gctggaacag gtaattgatc gccaccgcca ggtagtggtt cggattcatc
     4681 aaacctgccg gagtgacgat accgtgacgg tcataatccg ggtcgttagc aaacgccaga
     4741 tcgaacttat cacgcagtgc cagcaggccc gccatcgcac actcggagga gcagtccata
     4801 cggatcgcgc cgtctttatc aaggtgcata aagcggaagg tttgatcgac ctgatcgtta
     4861 acaatagtca ggttgaggtt gtaatactcg ccaatacgct tccagtattc gataccggaa
     4921 ccgcccagcg gatcaacgcc aagcgtcagg cccgctttct gaatcgccgc catatcaacg
     4981 atatcggcca gcccttccac aaacggctgc accagatcct gctctttcac atgaccggat
     5041 gccatcgctt cgtcgaggga gatacgcttc acgcctttca ggccatcagc cagcagtgcg
     5101 tttgctctgt cttccaccac tttagtgacg ttggtatcag ccgggccacc atttggcgga
     5161 ttgtacttga taccaccatc ttccggcggg ttatgggacg gtgtaatcac gataccgtct
     5221 gccagcgggc cacctttttt attgtgaacc agaatggcat tggaaatggc aggtgtcgga
     5281 gtgaagccat tgttttcctg cacaatgaca tcaacgccgt tcgctgccag cacttccagt
     5341 acggaaatga acgcaggctc ggacagggcg tgagtatctt tacccacata gcaagggcca
     5401 gtgatgccgt ttttcgcacg ttcttcagca attgcctgag cgatcgccag aatgtgcggc
     5461 tcgttaaagc tgtggcgcgc tgcactgcca cggtgaccgg aagtaccgaa tttcaccgcg
     5521 tgctccgcat tccctgcttc tggtttcagt acataatatt gcgccgtcag ttgggcgacg
     5581 ttaatcaaat cactctgttg tgcaggttgg cctgcacgat tgtggattgc cattgctttg
     5641 tcctttgtct gcaacgtttt agatagttcc gcaaaccttc tcaatcaatt ccgccgggaa
     5701 ttgcatcgac tgcatgatgt gttcgatcat gctgcattta cggccggtgt tggtgttggt
     5761 gatcacccaa tacggcgtac caggcacatg tttcggcttg gtctgattac catttttcag
     5821 cagcgtttgt tcatctgccg caaagtaaac gcgtgtacga ccgtgcaacg attccgttgc
     5881 ttcggcaaac gcctgggcgt caagagaata tagtgtagac aacagcagca taaagcgatt
     5941 gaccgctcgc ttttgctctg cgtattcgtc cgaaagcaga agttcacgca ttgcgcgaac
     6001 cttgtcttta atcgttttga ccggcttcgc ttcgacgata gcaggtgacg caacgcgaac
     6061 ctctttcgtc accggagcag caggctgtga tgcggcggta aatttcaaca tacgccgtaa
     6121 aatgtcggat gcgctctcgc cgatatgctt agtgtggctg gcaatatagc tgtagagttc
     6181 atcatcaact tcaatcgttt tcatcttaat ccagtgcagt gtcttagctg aatacaagtc
     6241 gccaggagta tatgaataaa tcccggcagc ggatagcgtc aagcctggct tgcagaaaaa
     6301 ctcggaataa accttatttg ttgcagccct gtggcacaat ggtcaacata ataccctaac
     6361 ccgacaacgc gaaaaaaaga actttgccat gaaattgaat atccgcgcgc aaactgcaca
     6421 aaaccagcac aataattctc ccatcgttct tgtccatggt ctgtttggca gcctcgacaa
     6481 ccttggcgta ctggctcgcg atctggtaaa cgatcacaat atcatccagg ttgatatgcg
     6541 taaccacggt ctttcaccga gagatccggt aatgaattac ccggcgatgg cgcaggatct
     6601 tgttgatacc ctggatgcac agcagatcga caaagcaaca tttatcggtc actccatggg
     6661 cggtaaagcg gtaatggctc ttactgcact agcccctgat cgcattgaca agctggtggc
     6721 gattgatatt gcgccagtcg attatcacgt tcgccgtcat gatgagattt tcgcggccat
     6781 caacgcagtc agtgaatcag acgcacaaac acgccagcaa gcagcggcaa taatgcgcca
     6841 gcatcttaat gaagaagggg tgattcagtt tctgttgaaa tcttttgttg acggggagtg
     6901 gcgctttaac gtgccagtat tgtgggatca gtatccgcat attgtgggtt gggagaaaat
     6961 cccggcatgg gatcatccta ccctgtttat ccctggcggc aattctccgt atgttagcga
     7021 gcagtaccgt gatgatttac tggcacaatt tccacaggct cgggcgcatg tgattgcagg
     7081 cgcgggtcac tgggtccatg ctgaaaaacc ggatgcggta ttacgcgcta tccgtcgcta
     7141 tctcaatgat taacgcaact gattaaaaac ccagcgtccg cgtcgctttc cgcgcgtgga
     7201 cgttggcgtg actcatcgcg ctgatgtatt atggcgcgct atccatccgg gcagaggcct
     7261 ggctgattgt ttcccccgaa gtcaccaaga tcatggccaa agaacaaacg gaccgtacga
     7321 cattagatct gttcgcgcac gagcgtcgac cgggacgacc gaaaactaat ccgctttcgc
     7381 gcgatgaaca gctgcgtatt aataaacgca accaactaaa acgcgacaaa gtacgtggcc
     7441 ttaagcgtgt cgaactgaag ctgaacgcgg aagctgtcga ggcgctgaac gagctggcgg
     7501 aatcgcgtaa tatgagccgt agcgaactga tcgaagagat gttaatgcag caactggcgg
     7561 cgctgcgtag tcagggcatc gtttaaattt ccactttcat gtagcacagt gtgcagtcct
     7621 gctcgtttgc tgattccgca agactgcctg ttctgctatg attgccttta tccgtgggca
     7681 attttccacc cccatttcaa taagtttcaa gaggttattt cactcatggc tatcactggc
     7741 atctttttcg gcagcgacac cggtaatacc gaaaatatcg caaaaatgat tcaaaaacag
     7801 cttggtaaag acgttgccga tgtccatgac attgcaaaaa gcagcaaaga agatctggaa
     7861 gcatatgaca ttctgctgct gggcatccca acctggtatt acggcgaagc gcagtgtgac
     7921 tgggatgact tcttcccgac tctcgaagag attgatttta atggtaaact ggttgcgctg
     7981 tttggttgtg gcgaccagga agattacgca gaatacttct gcgatgcatt gggcaccatc
     8041 cgcgacatca ttgaaccgcg cggcgcaact atcgttggtc actggccaac cgcgggctac
     8101 cacttcgaag cgtcaaaagg tctggctgat gatgaccact ttgtcggtct ggctatcgac
     8161 gaagatcgtc agccggaact gaccgctgaa cgtgtagaaa aatgggttaa acaaatttct
     8221 gaagagctgc atctcgacga aattctcaat gcctgatgtg atgcggcgta gactcatgtc
     8281 tacgccgtat taatagataa tgccaatcaa aataattgct acaaatttgt aacttttgct
     8341 gttgtacctg tacaatgtcc cggtgttcaa gtggccttgc cgttgtaaat gtaagctgtg
     8401 ccacgttttt attaacaata tttgccaggg acttgtagtt ttcatttagg cgtggcaatt
     8461 ctataatgat acgcattatc tcaagagcaa attctgtcac ttcttctaat gaagtgaacc
     8521 gcttagtaac aggacagatt ccgcatgact gataacaata ccgccctaaa gaaagctggc
     8581 ctgaaagtaa cgcttcctcg tttaaaaatc ctggaagttc ttcaggagcc ggacaaccat
     8641 cacgtcagtg cggaagattt atacaaacgt ctgatcgata tgggtgaaga aattggtctg
     8701 gctacggtat atcgcgtact gaaccagttt gacgacgctg gtatcgtcac ccgccacaat
     8761 tttgaaggcg gtaaatccgt atttgaactg acacagcaac atcaccacga tcacctgatc
     8821 tgcctcgact gcggtaaggt tatcgaattt agtgatgatt ccatcgaagc gcgtcagcgt
     8881 gaaatcgccg caaaacatgg cattcgcctg actaaccaca gtctctatct ttacggtcac
     8941 tgtgccgaag gcgattgccg cgaagatgag cacgcgcacg aaggcaaata agccagcctg
     9001 aacgagaaaa gccaacctgc aggttggctt ttttatacaa gggaaataag aaaagcgaga
     9061 gttacagctc tcacttattt gttattactg cgaatttcct gccagacctt atcacaatcg
     9121 gctttcaccg cctgatcatt tccggtttgc gttgcctgaa tgcactgctg ataatccagt
     9181 acgcggacac tttcctgctc ggcaaactgc tgatgttgtt tctctttctt cagcacgttt
     9241 aacacgctct ggcaggcttc aattttttcc ggcgaacctt gcgcggtgtt gatacaggcg
     9301 ctgtaagcct ctttcagacg actgtcttcc tgtggcgcgg ttgattgcgc acaagccacc
     9361 agccccgatg ccatcatggc gatgagaatc agttttttca tagcaaattc ctcaaacgtg
     9421 cggcaggcac aaaacctgcc gcatcgggca tcagaagatg gtgaatggtg cgattaccat
     9481 aaatttcacg tcacgctcat cctggaagat gttgccataa ccaccgcccc agcttgggat
     9541 gtcggagtgg ttgtcgtatt cggtgaagtg cagtttgaac atcgtgcctt tggcgcggcc
     9601 gtcctgaatg gtgtagaccg catccaggct gtaggcagac tcttcaatag tccggttttt
     9661 gtcgtagtac gcatccggat tgctctgcca ggtagcgggt ttagcatccc atgcgtaaac
     9721 gtaggaggcg ccgatggcaa aacctggaag attccagttt ttcaggtcat acatcgcgcc
     9781 gaagaagacc gctttttcgc cgttggcgtt gaagtcagaa cggttatccc accagatatc
     9841 caggcgaccg ttcgaggaag cgtaggttgg tgtcatacgt tgcaggaagt atccctgctg
     9901 accgtcggct ttaacccagg tgccttccag acgcaaatcc actacgtcag ccgcccggta
     9961 accaaaggtc aacgcctgca gccaggcggt gccgtcataa agatcgttgg cgctgcgatc
    10021 gtcaacttta tcgcgcgtgc catagaactg gtagctggtg gttaacgggc taccggcgat
    10081 atcaaatttg tagctggctt tggcaaaata ttgatcgata tacccttcgg cctgaccaaa
    10141 cgccgcttcc agtacgaagt tatttttgaa gtcgtacttc gccccaaggg agtgtagata
    10201 atcaactttg gtggttttat cgttctgata aaactcatcc atttccagat gccacggtgc
    10261 tttgtattcg ttggtccaca tgtaggagaa actcaacgca ccagcatcgc cgtaatcaaa
    10321 attcgccccg gcctccgccc cctgataagt acccggcata aagctccagt gtggcgctaa
    10381 cagcgtctga ccggttggct gaatataacc tgccctcgcc caaaccggac cgtatttaaa
    10441 tttggccgct gctttataca ggcttatacc gcttttatcg ccggaccagt cttcgtcata
    10501 ggctttatta ctttttgaaa acgcgatttc gttcgggtga gagctgtcgc cgttttccgc
    10561 catttcaatc gccgtaaacg cggcaatatc aaggccgaac atatcagcag cataaccaga
    10621 ctgaaaatcg aggttggcgt tccaggtgga gtgagaaagg ttggttttgt atttatcgcc
    10681 gtcggtaaca tctttacggt cgcgttcacg ctgccagtaa tagataccgc cggttaaggt
    10741 tgaatcgtcg atgaatcctt cggcgcgagc ctccggaatt gccataaagc ccgacatagc
    10801 tgtaacaccg gcgatagcca gcgccagcgt actacgtttg ccactaaacg cacgcatggg
    10861 ttaatcctct ttgacgtata aattgctgca ccaaaggtga acagcgaaaa aaataaaaat
    10921 taaaaaagta gcgttgagaa aagtctcgct gactacagga acagactatt tttcgcgacg
    10981 cgaattatca atctctttct gtaaccagaa tgtaagaaca tgagcaaccg cacatatttt
    11041 gatgattttt gttacaaggc ttaagtttgc agttgaaagg cttgcagaag aataaaaacg
    11101 tgaaattcca gcaagatgaa agcggttaca ttttatttaa ttatgttttg tgattttaaa
    11161 tttatt
//
LOCUS       DAHHTG010000131        11139 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.132, whole genome shotgun sequence.
ACCESSION   DAHHTG010000131 DAHHTG010000000
VERSION     DAHHTG010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11139)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11139)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11139
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.132"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            1..1122
                     /locus_tag="NBI74_003697"
     CDS             1..1122
                     /locus_tag="NBI74_003697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000581303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HCD1933974.1"
                     /translation="MKIEKVINKNDLKAFIAFPSSLYPDDPNWIPPLFIERNEHLSAK
                     NPGTDHIIWQAWVAKKAGQIVGRITAQIDTLHRERYGKDTGHFGMIDAIDDPQVFAAL
                     FGAAEAWLKSQGASKISGPFSLNINQESGLLIEGFDTPPCAMMPHGKPWYAAHIEQLG
                     YHKGIDLLEWWMQRTDLTFSPALKKLMDQVRKKVTIRCINRQRFAEEMQILREIFNSG
                     WQHNWGFVPFTEHEFATMGDQLKYLVPDDMIYIAEIDSAPCAFIVGLPNINEAIADLN
                     GSLFPFGWAKLLWRLKVSGVRTARVPLMGVRDEYQFSRIGPVIALLLIEALRDPFARR
                     KIDALEMSWILETNTGMNNMLERIGAEPYKRYRLYEKQI"
     gene            complement(1198..1968)
                     /gene="glcC"
                     /locus_tag="NBI74_003698"
     CDS             complement(1198..1968)
                     /gene="glcC"
                     /locus_tag="NBI74_003698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GlcC"
                     /protein_id="HCD1933975.1"
                     /translation="MKDERRPICEVVAESIERLIIDGVLKVGQPLPSERRLCEKLGFS
                     RSALREGLTVLRGRGIIETAQGRDSRVARLNRVQDTSPLIHLFSTQPRTLYDLLDVRA
                     LLEGESARLAATLGTQADFVVITRCYEKMLAASENNKEISLIEHAQLDHAFHLAICQA
                     SHNQVLVFTLQSLTDLMFNSVFASVNNLYHRPQQKKQIDRQHARIYNAVLQRLPHVAQ
                     RAARDHVRTVKKNLHDIELEGHHLIRSAVPLEMNKVGM"
     gene            2219..3718
                     /gene="glcD"
                     /locus_tag="NBI74_003699"
     CDS             2219..3718
                     /gene="glcD"
                     /locus_tag="NBI74_003699"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcD"
                     /protein_id="HCD1933976.1"
                     /translation="MSILYEERLDGALPDVDRTSVLMALREHVPGLEILHTDEEIIPY
                     ECDGLSAYRTRPLLVVLPKQMEQVTAILAVCHRLRVPVVTRGAGTGLSGGALPLEKGV
                     LLVMARFKEILDINPVGRRARVQPGVRNLAISQAVAPHNLYYAPDPSSQIACSIGGNV
                     AENAGGVHCLKYGLTVHNLLKIEVQTLDGEALTLGSDALDSPGFDLLALFTGSEGMLG
                     VTTEVTVKLLPKPPVARVLLASFDSVEKAGLAVGDIIANGIIPGGLEMMDNLSIRAAE
                     DFIHAGYPVDAEAILLCELDGVESDVQEDCEQVNDILLNAGATDVRLAQDEAERVRFW
                     AGRKNAFPAVGRISPDYYCMDGTIPRRALPGVLEGIARLSQQYDLRVANVFHAGDGNM
                     HPLILFDANEPGEFARAEELGGKILELCVEVGGSISGEHGIGREKINQMCAQFNSDEI
                     TTFHAVKAAFDPDGLLNPGKNIPTLHRCAEFGAMHVHHGHLPFPELERF"
     gene            3718..4770
                     /gene="glcE"
                     /locus_tag="NBI74_003700"
     CDS             3718..4770
                     /gene="glcE"
                     /locus_tag="NBI74_003700"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcE"
                     /protein_id="HCD1933977.1"
                     /translation="MLRECDYSQALLEQVNQAISDKTPLVIQGSNSKAFLGRPVTGQT
                     LDVRCHRGIVNYDPTELVITARAGTPLVAIEAALESAGQMLPCEPPHYGEEATWGGMI
                     ACGLAGPRRPWSGSVRDFVLGTRIITGTGKHLRFGGEVMKNVAGYDLSRLMAGSYGCL
                     GVLTEISMKVLPRPRATLSLRREISLQEAMNEIAQWQLQPLPISGLCYFDNALWIRLE
                     GGEGSVKAARELLGGEEVADQFWQQLREQQLPLFSLPGTLWRISLPSDAPMMDLPGEQ
                     LIDWGGALRWLKSTADDNQIHRIARNAGGHATRFSAGDGGFAPLPAPLFRYHQQLKQQ
                     LDPCGVFNPGRMYAEL"
     gene            4781..6004
                     /gene="glcF"
                     /locus_tag="NBI74_003701"
     CDS             4781..6004
                     /gene="glcF"
                     /locus_tag="NBI74_003701"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcF"
                     /protein_id="HCD1933978.1"
                     /translation="MQTQLTEEMRQNARALEADSILRACVHCGFCTATCPTYQLLGDE
                     LDGPRGRIYLIKQVLEGNDVTLKTQEHLDRCLTCRNCETTCPSGVRYHNLLDIGRDIV
                     EQKVKRPLPERMLREGLRQVVPRPAVFRALTQVGLVLRPFLPEQVRAKLPAETVKAKP
                     RPPLRHKRRVLMLEGCAQPTLSPNTNAATARVLARLGISVMPANEAGCCGAVDYHLNA
                     QEKGLARARNNIDAWWPAIEAGVEAILQTASGCGAFVKEYGQMLKNDALYADKARQVS
                     ELAVDLVELLREEPLEKLAIRGDKKLAFHCPCTLQHAQKLNGEVEKVLLRLGFTLTDV
                     PDSHLCCGSAGTYALTHPDLARQLRDNKMNALESGKPEMIVTANIGCQTHLASAGRTS
                     VRHWIEIVEQALEKE"
     gene            6009..6413
                     /locus_tag="NBI74_003702"
     CDS             6009..6413
                     /locus_tag="NBI74_003702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HCD1933979.1"
                     /translation="MKTKVILSQQMASAIIAAGQEEAQKNNWSVSIAVADDGGHLLAL
                     SRMDDCAPIAAYIAQEKARTAALGRRETKGYEEMVNNGRTAFVTAPLLTSLEGGVPVV
                     VDGQIIGAVGVSGLTGAQDAQVAKAAAAVLAK"
     gene            6435..8606
                     /gene="glcB"
                     /locus_tag="NBI74_003703"
     CDS             6435..8606
                     /gene="glcB"
                     /locus_tag="NBI74_003703"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase G"
                     /protein_id="HCD1933980.1"
                     /translation="MSQTIAQGRLRINANFKRFVDEEVLPGTGLDAAAFWRNFDEIVH
                     DLAPENRQLLAERDRIQAALDEWHRSNPGPVKDKAAYKSFLRELGYLVPQPERVTVET
                     TGIDSEITSQAGPQLVVPAMNARYALNAANARWGSLYDALYGSDIIPQEGAMVSGYDP
                     QRGEQVIAWVRRFLDESLPLENGSYQDVVAFKVVDKQLRIQLKNGKETTLRTPAQFVG
                     YRGDAAAPTCILLKNNGLHIELQIDANGRIGKDDPAHINDVIVEAAISTILDCEDSVA
                     AVDAEDKILLYRNLLGLMQGTLQEKMEKNGRQIVRKLNDDRHYTAADGSEISLHGRSL
                     LFIRNVGHLMTIPVIWDSEGNEIPEGILDGVMTGAIALYDLKVQKNSRTGSVYIVKPK
                     MHGPQEVAFANKLFTRIETMLGMAPNTLKMGIMDEERRTSLNLRSCIAQARNRVAFIN
                     TGFLDRTGDEMHSVMEAGPMLRKNQMKSTPWIKAYERNNVLSGLFCGLRSKAQIGKGM
                     WAMPDLMADMYSQKGDQLRAGANTAWVPSPTAATLHALHYHQTNVQSVQANIAQSEFN
                     AEFEPLLDDLLTIPVAENANWSVEEIQQELDNNVQGILGYVVRWVEQGIGCSKVPDIH
                     NVALMEDRATLRISSQHIANWLRHGILTKEQVQASLENMAKVVDQQNAGDPAYRPMAG
                     NFANSCAFKAASDLIFLGVKQPNGYTEPLLHAWRLREKESH"
     gene            8961..10643
                     /gene="glcA"
                     /locus_tag="NBI74_003704"
     CDS             8961..10643
                     /gene="glcA"
                     /locus_tag="NBI74_003704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate permease GlcA"
                     /protein_id="HCD1933981.1"
                     /translation="MVTWTQMYMPMGGLGLSALVALIPIIFFFVALAVLRLKGHVAGA
                     ITLILSILIAIFAFKMPIDMAFAAAGYGFIYGLWPIAWIIVAAVFLYKLTVASGQFDI
                     IRSSVISITDDQRLQVLLIGFSFGALLEGAAGFGAPVAITGALLVGLGFKPLYAAGLC
                     LIANTAPVAFGALGVPILVAGQVTGIDPFHIGAMAGRQLPFLSVLVPFWLVAMMDGWK
                     GVKETWPAALVAGGSFAVTQFFTSNYIGPELPDITSALVSIVSLALFLKVWRPKNTET
                     AISMGQSAGAMVVNKPSSGGPVPSEYSLGQIIRAWSPFLILTVLVTIWTMKPFKALFA
                     PGGAFYSLVINFQIPHLHQQVLKAAPIVAQPTPMDAVFKFDPLSAGGTAIFIAAIISI
                     FILGVGIKKGIGVFAETLISLKWPILSIGMVLAFAFVTNYSGMSTTLALVLAGTGVMF
                     PFFSPFLGWLGVFLTGSDTSSNALFGSLQSTTAQQINVSDTLLVAANTSGGVTGKMIS
                     PQSIAVACAATGMVGRESELFRYTVKHSLIFASVIGIITLLQAYVFTGMLVS"
BASE COUNT         2621 a         2771 c         3109 g         2638 t
ORIGIN      
        1 attaaaattg aaaaagtaat taataaaaat gaccttaagg catttatcgc ttttccgtca
       61 tcactttatc ccgacgatcc aaactggata ccccctttat tcattgaacg caacgagcat
      121 ttgtctgcga aaaatccggg gacggaccat atcatctggc aggcgtgggt ggcaaaaaaa
      181 gcggggcaga tagttgggcg tattaccgcg caaatagata ccttgcaccg cgagcgttac
      241 ggcaaagata ccggtcattt cggcatgatt gacgccattg atgatccgca ggtttttgcc
      301 gcgttgtttg gcgcagcgga agcgtggttg aagtcacaag gtgcaagtaa gatcagcggg
      361 cctttcagcc tgaatatcaa tcaggaaagc ggattactga ttgaaggttt cgatacacca
      421 ccctgtgcga tgatgccgca cggtaaaccg tggtatgccg cgcatattga acaattgggt
      481 tatcacaaag gcattgattt actggagtgg tggatgcagc gaaccgatct cactttctct
      541 ccggcgctaa aaaaactgat ggatcaggtg cgtaagaagg tgaccattcg ctgcatcaat
      601 cgccagcggt ttgccgaaga gatgcagatc ctgcgtgaga ttttcaattc tggctggcag
      661 cacaactggg gatttgtgcc gtttaccgaa catgaattcg cgaccatggg cgaccaactt
      721 aaatatctgg tgccggacga tatgatctac atcgctgaga ttgattctgc accctgcgcg
      781 tttattgtcg gcttgccaaa catcaacgag gcgattgccg atctgaacgg atcgctcttt
      841 cccttcggct gggcaaaatt gctgtggcgt ttaaaagtca gcggtgtgcg aactgcgcga
      901 gtaccgctga tgggcgtgcg ggacgagtat cagttcagcc gcatcggccc ggtgattgcg
      961 ttgttattga ttgaagcctt acgcgatccg tttgcccgcc ggaagatcga tgcgctggag
     1021 atgtcatgga tcctcgaaac caatacaggc atgaataaca tgctggaacg cattggggca
     1081 gagccgtaca agcgttaccg attgtatgaa aagcagattt aataccagat cctgttaagc
     1141 gcaggggcga tccctcgccc ctcggggaaa gggttagggt gagggtgaaa actcggctca
     1201 cattcccacc ttgttcatct ccagcggcac cgccgagcga atcaaatggt ggccttccag
     1261 ctcgatatcg tggagattct ttttcacggt ccgcacatga tcgcgtgctg cgcgctgggc
     1321 gacgtgcggc agccgctgca acaccgcgtt gtagatccgc gcatgctggc gatcgatctg
     1381 ctttttttgc tgtggtcgat ggtagagatt atttacgctg gcaaacactg aattaaacat
     1441 cagatcggtc aatgattgca gcgtaaacac caacacctga ttgtgagaag cctgacaaat
     1501 ggcgagatgg aaagcgtgat ccaactgcgc atgttcgatc agcgaaatct ctttgttgtt
     1561 ctcactggcg gcgagcattt tttcataaca gcgggttatc acaacaaaat cagcctgggt
     1621 tcccagcgtt gccgccagcc ttgccgattc accctccagc aatgcgcgaa cgtcgagcag
     1681 atcgtacagc gttcgcggct gcgtactgaa cagatggatc agcgggctgg tgtcctgcac
     1741 ccgattaagc cgtgcgacac gagaatcgcg accctgcgcc gtttcaataa tcccgcgccc
     1801 gcgcagcacg gtcagccctt cacgcagtgc ggagcgtgag aagccgagct tttcacacag
     1861 tcgacgttcc gagggaagcg gctgaccgac cttcagtacg ccgtcgataa ttaaccgttc
     1921 gatactctct gcaaccactt cgcaaatagg gcgacgttca tctttcattc ccggacctcg
     1981 tgcacaggta ggaccaattt ttctgttctt aactcgcaaa acacgcacat cacgtaagtg
     2041 attatgttac atcaatttaa cattaagtta accaagacaa ggtcacagag ctggaaaaaa
     2101 aatggtctga ccggtagcta aagagataga cgaaaacgaa aagcccgctt aataactgtt
     2161 cacagaagca gcgcgaaaaa atcagctgcc acacaacaca acaaagcgaa ccctactcat
     2221 gagcatcttg tacgaagagc gtcttgatgg cgctttaccc gatgtcgacc gcacatcggt
     2281 actgatggca ctgcgtgagc atgtccctgg actggagatc ctgcataccg atgaggagat
     2341 cattccttac gagtgtgacg ggttgagcgc gtatcgcacg cgtccattac tggttgtttt
     2401 gcctaagcaa atggaacagg tgacagcgat tctggctgtt tgtcaccgcc tgcgtgtacc
     2461 ggtggtgacc cgtggtgcag gcaccgggct ttctggtggc gcgctgccgc tggaaaaagg
     2521 tgtgttgttg gtgatggcgc gctttaaaga gatcctcgac attaaccccg ttggtcgccg
     2581 tgcgcgcgtg cagccgggcg tgcgtaacct ggcgatctcc caggccgttg cgccgcataa
     2641 tctctattac gcgccggatc cgtcctcaca aatcgcctgt tcgattggcg gcaatgtggc
     2701 ggaaaatgcc ggtggcgttc actgcctgaa atatggtcta accgtacata acctgctgaa
     2761 aattgaagtg caaacgctgg atggcgaggc gctgacgctg ggatcggacg cgctggattc
     2821 gcctggtttt gacctgctgg cgctgtttac cggatcggaa ggtatgctcg gcgtgaccac
     2881 cgaagtaacg gtgaagctgt tgccgaagcc gcccgtggcg cgggttctgt tagccagctt
     2941 tgactcggta gaaaaagccg gacttgcggt tggtgacatc atcgccaatg gcattatccc
     3001 cggcgggctg gagatgatgg ataacctgtc gattcgcgcg gcggaagatt ttattcatgc
     3061 cggttatccc gtcgatgccg aagcgatttt gttatgcgaa ctggacggcg tggagtctga
     3121 cgtacaggaa gactgcgagc aggttaacga catcttgttg aacgcgggtg cgactgacgt
     3181 ccgtctggca caggacgaag cagagcgagt acgtttctgg gccggtcgca aaaatgcgtt
     3241 cccggcggta ggacgtatct ccccggatta ctactgcatg gatggcacca tcccgcgccg
     3301 cgccctgcct ggcgtactgg aaggcattgc ccgtttatcg cagcaatatg atttacgcgt
     3361 tgccaacgtc tttcatgccg gagacggcaa catgcacccg ttaatccttt tcgatgccaa
     3421 cgaacccggt gaatttgccc gcgcggaaga actgggcggg aagatcctcg aactctgcgt
     3481 tgaagttggc ggcagcatca gtggggaaca tggcatcggg cgcgaaaaaa tcaatcaaat
     3541 gtgcgcccag ttcaacagcg atgaaatcac gaccttccat gcggtcaagg cggcgtttga
     3601 ccccgatggt ttgctgaacc caggaaaaaa cattcccacg ctacaccgct gtgctgaatt
     3661 tggtgccatg catgtgcatc acggtcattt accttttcct gaactggagc gtttctgatg
     3721 ctacgcgagt gtgattacag ccaggcgctg ctggagcagg tgaatcaggc gattagcgat
     3781 aaaacgccgc tggtgattca gggcagcaat agcaaagcct ttttaggtcg tcctgtcacc
     3841 gggcaaacgc tggatgtgcg ttgtcatcgc ggcattgtta attacgaccc gaccgagctg
     3901 gtgataaccg cgcgtgccgg aacgccgctg gtggcgattg aagcggcgct ggagagcgcc
     3961 gggcaaatgc tcccctgcga gccgccgcat tatggtgaag aagccacctg gggcgggatg
     4021 atcgcctgcg ggctggcggg gccgcgtcgc ccgtggagcg gttcggtccg cgattttgtc
     4081 ctcggcacgc gcatcattac cggcactgga aaacatctgc gttttggtgg cgaagtgatg
     4141 aaaaacgttg ccggatacga tctctcacgg ttaatggccg gaagctacgg ttgtctgggt
     4201 gtgctcactg aaatctcgat gaaagtgtta ccgcgaccac gcgccaccct gagtctgcgt
     4261 cgggaaatca gcctgcaaga agccatgaat gaaatcgccc agtggcaact ccagccatta
     4321 cccattagtg gcttatgtta cttcgacaat gcgttgtgga tccgccttga gggcggcgaa
     4381 ggatcggtaa aagcagcgcg tgaactgctg ggtggcgaag aggttgcgga tcagttctgg
     4441 cagcaattgc gtgaacaaca actgccgctc ttctcgttac caggtacctt atggcgcatt
     4501 tcattaccca gcgatgcgcc gatgatggat ttacccggcg agcaactgat cgactggggc
     4561 ggggcgttac gctggctgaa atcgacagcc gatgacaatc aaatccatcg cattgcccgc
     4621 aacgctggcg gtcatgcgac ccgctttagt gccggagatg gtggttttgc cccgctaccg
     4681 gctcctttat tccgctatca ccagcagctt aaacagcagc tcgacccttg cggcgtgttt
     4741 aatcccggtc gcatgtacgc ggagctttaa ggagcaggct atgcaaaccc aattaaccga
     4801 agagatgcgg cagaacgcgc gcgcgctgga agccgacagc atcctgcgcg cctgtgttca
     4861 ctgcggattt tgtaccgcaa cctgcccaac ctatcagctt ctgggcgatg aactggacgg
     4921 gccgcgcggg cgcatctatc tgattaaaca ggtgctggaa ggcaacgacg tcacgcttaa
     4981 aacgcaggaa catctcgatc gctgtctcac ttgccgtaat tgtgaaacca cctgtccttc
     5041 tggtgtgcgc tatcacaatt tgctggatat cgggcgtgat attgtcgagc agaaagtgaa
     5101 acgcccattg ccggagcgaa tgctgcgcga aggattgcgc caggtagtgc cgcgtccggc
     5161 ggtcttccgt gcgctgacgc aggtaggact ggtgctgcga ccgtttctgc cggaacaggt
     5221 cagagcaaaa ctgcccgccg aaacggtgaa agctaaaccg cgaccaccgc tgcgccataa
     5281 gcgtcgggtt ttaatgctgg aaggctgcgc ccagcctacg ctttcgccca acaccaacgc
     5341 ggcaaccgcg cgagtgctgg ctcgtctggg gatcagcgtc atgccagcta acgaagcagg
     5401 ctgttgtggc gcggtggact atcatcttaa tgcgcaagag aaagggctgg cgcgggcgcg
     5461 aaataatatt gatgcctggt ggcccgcgat tgaagcaggt gtcgaggcaa ttttgcaaac
     5521 cgccagcggt tgtggcgcgt tcgtcaaaga gtatgggcag atgctgaaaa acgatgcgtt
     5581 gtatgccgat aaagcgcgtc aggttagtga gctggcggtc gatttagtcg aacttctgcg
     5641 cgaggaaccg ctggaaaaac tggcaattcg cggcgataaa aaactggcct tccactgccc
     5701 gtgtacccta caacatgcgc aaaagctgaa cggcgaagtg gaaaaagtgt tacttcgtct
     5761 tggatttacc ttaacggacg ttcccgacag ccatctgtgc tgcggttcag cgggaacata
     5821 tgcgttaacg catcccgatc tggcacgcca gctgcgggat aacaaaatga atgcgctgga
     5881 aagcggcaaa ccggaaatga tcgtcaccgc caacattggt tgccagacgc atctggcgag
     5941 cgccggtcgt acctctgtgc gtcactggat tgaaattgta gaacaagccc ttgaaaagga
     6001 ataacaaaat gaaaactaaa gtcattctta gccagcaaat ggcgagtgca attattgccg
     6061 caggtcagga agaggcgcag aaaaataact ggtctgtttc cattgctgtt gccgatgacg
     6121 gcggtcatct gctggcgtta agccgcatgg acgattgcgc gccgattgcg gcttacatcg
     6181 cccaggagaa agcgcgtacc gccgcgctgg ggcgtcgtga aactaagggc tatgaagaga
     6241 tggtgaacaa cggacgtacc gcgttcgtga ctgcgccgtt actgacgtcg ctggaaggcg
     6301 gcgtaccggt tgttgtggat gggcaaatta ttggtgccgt gggcgtttct ggtttaaccg
     6361 gagcacagga tgcgcaggtc gcgaaagcgg cagcagcggt gttggcgaaa taagcgaaaa
     6421 cgaggagaga aacaatgagt caaaccatag cccagggccg tttacgcatt aacgctaatt
     6481 ttaaacgttt tgtggatgaa gaagttttac cgggaacagg gttggacgct gcggcgttct
     6541 ggcgcaattt tgatgagatc gttcacgatc tggcaccaga aaatcgtcag ttgctggcag
     6601 aacgcgatcg cattcaggcg gcgcttgatg agtggcatcg cagcaatccg gggccggtta
     6661 aagataaagc ggcctataaa tctttcctgc gtgaactggg ctacctggtg ccacaaccgg
     6721 agcgcgtgac ggtggaaacc acgggcattg acagcgaaat caccagccag gcggggccac
     6781 agctggtggt tccggcgatg aacgcccgct acgcgctgaa cgcggcgaac gctcgctggg
     6841 gctcactgta cgatgcgtta tacggcagcg acatcatccc gcaggaaggg gcgatggtca
     6901 gcggctacga tccgcaacgc ggtgagcagg ttatcgcctg ggttcggcgt ttcctcgatg
     6961 aatcactacc gctggaaaac ggcagctatc aggatgtggt ggcgtttaag gtggtcgata
     7021 aacaattacg catccagttg aaaaatggta aagaaaccac gttacgtacc ccggcgcagt
     7081 ttgtcggtta ccgtggcgat gccgctgcgc cgacctgcat tttgctgaaa aataacggtc
     7141 tgcatattga actgcaaatt gatgccaacg ggcggattgg caaagacgat ccggcgcata
     7201 tcaacgatgt tatcgtcgaa gcggccatca gtaccattct cgactgcgaa gattcggtcg
     7261 cggcggttga tgcggaagat aaaatcctgc tgtaccgcaa cctgctgggc ctgatgcagg
     7321 ggactctgca agagaaaatg gaaaaaaacg gtcggcaaat cgtgcgtaaa ctgaatgacg
     7381 atcgtcatta caccgccgcc gatggctctg aaatttctct gcacggacgc tcgctgctgt
     7441 ttatccgcaa cgtgggtcat ttgatgacca ttcctgtgat ttgggacagc gaaggcaatg
     7501 aaatcccgga aggcattctt gatggcgtca tgactggcgc gattgccctc tatgatttaa
     7561 aagtgcagaa aaactcgcgc actggcagcg tctatattgt gaaaccgaaa atgcacggtc
     7621 cgcaggaagt ggcgttcgcc aacaaactgt ttacccgcat tgagacaatg ctcggtatgg
     7681 caccgaatac cctgaaaatg ggcattatgg atgaagaacg tcggacctcg ctgaacttgc
     7741 gtagctgtat cgctcaggcg cgcaaccgcg tggcgttcat caataccggt ttcctcgacc
     7801 gtaccggcga tgaaatgcat tcggtgatgg aagctggccc gatgctgcgt aaaaatcaga
     7861 tgaaatcgac gccttggatc aaagcctacg agcgtaataa cgtgctttcc ggcctgttct
     7921 gtgggctgcg cagtaaagcg caaatcggta aaggcatgtg ggcaatgccg gacctgatgg
     7981 cagacatgta cagccagaag ggcgaccaac tgcgtgccgg ggcaaacaca gcctgggttc
     8041 cgtcaccaac cgctgctacg ctccatgcgc tgcactacca ccaaaccaac gtacagagcg
     8101 tgcaggccaa cattgcccag agcgagttca atgctgaatt tgaaccgctg ctggacgatc
     8161 tgctgactat tccggtggct gaaaacgcta actggtcggt ggaagagatc caacaagagc
     8221 tggataacaa cgtgcagggg attctggggt acgtggtgcg ttgggtggag caggggattg
     8281 gttgttcaaa agtgccggat attcacaacg tggcgctgat ggaagaccgc gcaacgctgc
     8341 gtatctccag ccagcatatc gccaactggt tacgtcacgg tattctgacc aaagaacagg
     8401 tgcaggcgtc gctggagaat atggcgaaag tggttgatca gcaaaacgct ggcgatccgg
     8461 cttatcgtcc gatggcgggg aatttcgcta actcgtgtgc ttttaaagct gccagcgatt
     8521 taatcttcct cggcgtgaaa cagccaaacg gctataccga accgttatta cacgcctggc
     8581 gtttacgcga aaaagaaagt cattaatagc gtcaaaaaag ccccggcaag ttttattgtc
     8641 ggggctatga tagtaactgc ggtagtacat ttatcccgga aataaccgga gcgattatca
     8701 gggtttaaca atattaaata cccgtagcgc attactagcg ggcctaaaac aacaatatgg
     8761 acatcttaac gatattctgc gcctgcgtgt gcggaacaga aacccttacc cgatatactt
     8821 gttatgccag aggtagcaat tgcgttagca gcgtactgtt gctgtaaagt ataacgccta
     8881 tattttacag ttcagtcctc ttttgcttat tacaacctta agtatatcaa gcatataaag
     8941 ataataagag actgaaaaat atggttacct ggacccaaat gtatatgccg atgggaggac
     9001 tggggctatc cgctctggtc gccctgatcc cgataatatt cttcttcgtt gcactcgcgg
     9061 tattacgtct gaaaggacat gtcgccggag caataaccct tatattatct attctgattg
     9121 cgatattcgc ctttaaaatg ccgattgata tggcatttgc tgctgcgggc tatggcttta
     9181 tttatggatt atggcctatt gcgtggatta ttgttgcggc ggtgttcctc tataaattaa
     9241 ccgttgccag cggtcagttc gatattatcc gcagctcggt tatctccatc actgacgatc
     9301 agcgtttgca ggtgttactg attggtttct cctttggcgc gctgctggaa ggggcggctg
     9361 gctttggtgc gccggtggcg attaccggtg cgctgctggt gggcctgggc ttcaaaccgt
     9421 tatacgcggc ggggctgtgt ctgattgcca acactgcgcc ggtggcgttt ggtgcgttgg
     9481 gcgtgccgat tctggtcgcc ggtcaggtaa cgggaatcga tccgttccac attggcgcaa
     9541 tggcgggacg tcagttaccg ttcctgtcgg ttcttgtgcc gttctggctg gtagcaatga
     9601 tggacggctg gaaaggggtg aaagagacgt ggccagcggc gctggttgct gggggaagct
     9661 tcgctgtcac gcagttcttt acctctaact atattggtcc ggaactgccg gatattactt
     9721 cggcgctggt gagtatcgtc tcactcgctt tattccttaa agtctggcgg ccgaaaaata
     9781 ccgaaacggc aatcagcatg ggacaatccg caggtgcgat ggtggtgaat aaaccgtctt
     9841 ctggcggtcc cgtgccttca gaatatagtc tggggcaaat cattcgtgcg tggtcaccgt
     9901 ttttaatctt aacggtgctg gtcaccatct ggaccatgaa gccgtttaaa gcgttatttg
     9961 ctccgggcgg cgcgttttat tcactggtga ttaatttcca gatccctcat ttgcatcaac
    10021 aagtgttgaa agcggcaccc attgtcgccc aaccaacgcc aatggatgcg gtgtttaaat
    10081 tcgaccctct ctcggctggc ggcaccgcta tttttattgc ggcgattatc tctatcttca
    10141 tcctcggcgt gggcatcaag aaagggattg gcgtctttgc cgaaacgcta attagtctga
    10201 agtggccgat attgtcgatt ggcatggtgc tggcgttcgc cttcgtcacc aactattccg
    10261 gcatgtccac cacgctggcg ctggtactgg caggtacagg cgtgatgttc ccgtttttct
    10321 caccgtttct cggctggctg ggcgtattcc ttaccggctc ggacacctcc tctaacgccc
    10381 tgtttggttc actgcaatcg accacggcgc agcaaatcaa cgtctctgac actctgctgg
    10441 tggcagcaaa taccagcggc ggcgtaactg gcaagatgat ttccccgcaa tctatcgccg
    10501 tggcctgcgc cgcgacgggc atggtgggcc gagaatctga actgttccgc tacaccgtga
    10561 agcacagtct gatctttgcc agtgtgatcg gcattatcac gctgttgcag gcgtatgtgt
    10621 ttaccgggat gttagtttcg taagtcaata aagccggatg atcgttaata tcatccggct
    10681 tcgtttacat tactctgtct tattaatgga aaattatttc ccgctttatt atccatgtgg
    10741 caataatgtt tcgcgttcag ataacctgct aaataattaa aagcgtgaga acatctgttt
    10801 ttttatcacg caaaacaagt cagtattaat tatgttcgct tgatgagcaa acaccgttct
    10861 atctgttgct tttgcaatct taatcgttaa aactaccaac aaatttatat agttacgttg
    10921 tgaaaagttg ttgatttttt gtttattgaa atttcgtaaa aataaccagc cagacatcga
    10981 atagggggta ttcttataaa tttcacctgt tttctatcaa tgatgccgtt ttcttaagaa
    11041 tggaggaagt tattaaagaa gataaagcat aaaactattt ccccggttac gaatctttta
    11101 actttgtttt gtcacttgcg ttattaatga ataagaaat
//
LOCUS       DAHHTG010000132        11020 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.133, whole genome shotgun sequence.
ACCESSION   DAHHTG010000132 DAHHTG010000000
VERSION     DAHHTG010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11020)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11020)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11020
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.133"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            complement(<1..1496)
                     /locus_tag="NBI74_003705"
     CDS             complement(<1..1496)
                     /locus_tag="NBI74_003705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lon protease family protein"
                     /protein_id="HCD1933982.1"
                     /translation="MTITKLAWRDLVPDTDSYQEIFAQPHLIDENDPLFSDTQPRLQF
                     ALEQLLHTRASSSFMLAKAPEESEYLNLIADAARTLQSDAGQLVGGHYEVSGHTIRLR
                     HAVSADDNFATLTQVVAADWVEAEQLFGCLRQFNGDITLQPGLVHQANGGILIISLRT
                     LLAQPLLWMRLKNIVNRERFDWVAFDESRPLPVSVPSMPLKLKVILVGERESLADFQE
                     MEPELSEQAIYSEFEDTLQIVDAESVSQWCRWVTFTARHNHLPAPGADAWPVLIREAA
                     RYTGEQETLPLSPQWILRQCQEVASLCDGDTFSGEQLNLMLQQREWREGFLAERMQDE
                     ILQEQILIETEGERIGQINALSVIEFPGHPRAFGEPSRISCVVHIGDGEFTDIERKAE
                     LGGNIHAKGMMIMQAFLMSELQLEQQIPFSASLTFEQSYSEVDGDSASMAELCALISA
                     LADVPVNQSIAITGSVDQFGRAQPVGGLNEKIEGFFAICQQRELTGKQG"
     gene            1682..2134
                     /gene="matP"
                     /locus_tag="NBI74_003706"
     CDS             1682..2134
                     /gene="matP"
                     /locus_tag="NBI74_003706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ter protein MatP"
                     /protein_id="HCD1933983.1"
                     /translation="MKYQQLENLESGWKWKYLVKKHREGELITRYIEASAAQEAVDEL
                     LSLENEPVLVNGWIDKHMNPELVNRMKQTIRARRKRHFNAEHQHTRKKSIDLEFIVWQ
                     RLAGLAQRRGKTLSETIVQLIEDAENKEKYANKMSSLKQDLQALLGKE"
     gene            complement(2210..3262)
                     /gene="ompA"
                     /locus_tag="NBI74_003707"
     CDS             complement(2210..3262)
                     /gene="ompA"
                     /locus_tag="NBI74_003707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706879.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpA"
                     /protein_id="HCD1933984.1"
                     /translation="MKKTAIAIAVALAGFATVAQAAPKDNTWYTGAKLGWSQYHDTGF
                     IPNNGPTHENQLGAGAFGGYQVNPYVGFEMGYDWLGRMPYKGDNINGAYKAQGVQLTA
                     KLGYPITDDLDIYTRLGGMVWRADTKANVPGGASFKDHDTGVSPVFAGGVEYAITPEI
                     ATRLEYQWTNNIGDAHTIGTRPDNGMLSLGVSYRFGQGEAAPVVAPAPAPAPEVQTKH
                     FTLKSDVLFTFNKATLKPEGQAALDQLYSQLSNLDPKDGSVVVLGYTDRIGSDAYNQA
                     LSERRAQSVVDYLISKGIPADKISARGMGESNPVTGNTCDNVKQRAALIDCLAPDRRV
                     EIEVKGIKDVVTQPQA"
     gene            complement(3618..4127)
                     /gene="sulA"
                     /locus_tag="NBI74_003708"
     CDS             complement(3618..4127)
                     /gene="sulA"
                     /locus_tag="NBI74_003708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibitor SulA"
                     /protein_id="HCD1933985.1"
                     /translation="MYTSGYAHRSSSFSSAASKIARVSTENTTAGLISEVVYREDQPM
                     MTQLLLLPLLQQLGQQSRWQLWLTPQQKLSREWVQASGLPLTKVMQISQLSPCHTVES
                     MVRALRTGNYSVVIGWLADDLTAEEHAELVDAANEGNAMGFIMRPVSASSHATRQLSG
                     LKIHSNLYH"
     gene            4346..4975
                     /gene="sxy"
                     /locus_tag="NBI74_003709"
     CDS             4346..4975
                     /gene="sxy"
                     /locus_tag="NBI74_003709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRP-S regulon transcriptional coactivator Sxy"
                     /protein_id="HCD1933986.1"
                     /translation="MKSLSYKRIYKSQEYLATLGTIEYRSLFGSYSLTVDDTVFAMVS
                     DGELYLRACEQSAQYCVKHPPVWLTYKKCGRSVTLNYYRVDESLWRNQLKLVRLSKYS
                     LDAALKEKSTRNTRERLKDLPNMSFHLEAILGEVGIKDVRALRILGAKMCWLRLRQQN
                     CLVTEKILFMLEGAIIGIHEAALPVARRQELAEWADSLTPKQEFPAELE"
     gene            complement(4938..7100)
                     /gene="yccS"
                     /locus_tag="NBI74_003710"
     CDS             complement(4938..7100)
                     /gene="yccS"
                     /locus_tag="NBI74_003710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309071.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01666 family membrane protein"
                     /protein_id="HCD1933987.1"
                     /translation="MAFMLSPLLKRYTWNSAWLYYARIFIALCGTTAFPWWLGDVKLT
                     IPLTLGMVAAALTDLDDRLAGRLRNLIITLFCFFIASASVELLFPWPWLFAIGLTLST
                     SGFILLGGLGQRYATIAFGALLIAIYTMLGTSLYEHWYQQPMYLLAGAVWYNVLTLIG
                     HLLFPVRPLQDNLARCYEQLARYLELKSRMFDPDIEDESQAPLYDLALANGQLMATLN
                     QTKLSLLTRLRGDRGQRGTRRTLHYYFVAQDIHERASSSHIQYQTLREHFRHSDVLFR
                     FQRLMSMQGQACQQLSRCILLRQPYQHDPHFERAFTHIDAALERMRDNGAPADLLKTL
                     GFLLNNLRAIDAQLATIESEQAQALPHNNDENELADDSPHGLSDIWLRLSRHFTPESA
                     LFRHAVRMSLVLCFGYAIIQITGMHHGYWILLTSLFVCQPNYNATRHRLKLRIIGTLV
                     GIAIGIPVLWFVPSLEGQLVLLVITGVLFFAFRNVQYAHATMFITLLVLLCFNLLGEG
                     FEVALPRVIDTLIGCAIAWAAVSYIWPDWKFRNLPRMLERATEANCRYLDAILEQYHQ
                     GRDNRLAYRIARRDAHNRDAELASVVSNMSSEPNVTPQIREAAFRLLCLNHTFTSYIS
                     ALGAHREQLTNPEILAFLDDAVCYVDDALHHQPADEERVNQALAGLKQRMQQLEPRAD
                     SKEPLVVQQVGLLIALLPEIGRLQRQITQVPQETPVSA"
     gene            complement(7110..7556)
                     /locus_tag="NBI74_003711"
     CDS             complement(7110..7556)
                     /locus_tag="NBI74_003711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HCD1933988.1"
                     /translation="MRTVLNILNFVLGGFATTLGWLLATLVSIVLIFTLPLTRSCWEI
                     TKLSLVPYGNEAIHVDELNPAGKNVLLNTGGTVLNIFWLIFFGWWLCLMHIATGIAQC
                     ISIIGIPVGIANFKIAAIALWPVGRRVVSVETAQAAREANARRRFE"
     gene            7679..9733
                     /gene="helD"
                     /locus_tag="NBI74_003712"
     CDS             7679..9733
                     /gene="helD"
                     /locus_tag="NBI74_003712"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase IV"
                     /protein_id="HCD1933989.1"
                     /translation="MELKATTLGKRLAQHPYDRAVILNAGIKVSGDRHEYLIPFNQLL
                     AIHCKRGLVWGELEFVLPDEKVVRLHGTEWGETQRFYHHLDAHWRRWSGEMSEIASGV
                     LRQQLDLIATRTGENKWLTREQTSGMQQQIRQALSALPLPVNRLEEFDNCREAWRKCQ
                     AWLKDIEGARLQHNQAYTEAMLTEYADFFRQVESSPLNPAQARAVVNGEHSLLVLAGA
                     GSGKTSVLVARAGWLLARGEASPEQILLLAFGRKAAEEMDERIRERLHTEDITARTFH
                     ALALHIIQQGSKKVPIVSKLENDTAARHELFIAEWRKQCSEKKAQAKGWRQWLTEEMQ
                     WSVPEGNFWDDEKLQRRLASRLDRWVSLMRMHGGAQAEMIASAPEEIRDLFSKRIKLM
                     APLLKAWKGALKAENAVDFSGLIHQAIVILEKGRFISPWKHILVDEFQDISPQRAALL
                     AALRKQNSQTTLFAVGDDWQAIYRFSGAQMSLTTAFHENFGEGDRCDLDTTYRFNSRI
                     GEVANRFIQQNPGQLKKPLNSLTNGDKKAVTLLDEGQLDALLDKLSGYAKPEERILIL
                     ARYHHMRPASLEKAATRWPKLQIDFMTIHASKGQQADYVIIVGLQEGSDGFPAAARES
                     IMEEALLPPVEDFPDAEERRLMYVALTRARHRVWALFNKENPSPFVEILKNLDVPVAR
                     KP"
     gene            complement(9765..10223)
                     /gene="mgsA"
                     /locus_tag="NBI74_003713"
     CDS             complement(9765..10223)
                     /gene="mgsA"
                     /locus_tag="NBI74_003713"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HCD1933990.1"
                     /translation="MELTTRTLPSRKHIALVAHDHCKQMLMSWVERHQPLLEQHVLYA
                     TGTTGNLISRATGMNVNAMLSGPMGGDQQVGALISEGKIDVLIFFWDPLNAVPHDPDV
                     KALLRLATVWNIPVATNVATADFIIQSPHFNDAVDILIPDYQRYLADRLK"
     gene            complement(10319..10981)
                     /locus_tag="NBI74_003714"
     CDS             complement(10319..10981)
                     /locus_tag="NBI74_003714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2057 family protein"
                     /protein_id="HCD1933991.1"
                     /translation="MKTGIVTTLIALCLPVSVFATTLRLSTDVDLLVLDGKKVSSSLL
                     RGADSIELDNGPHQLVFRVEKTIHLSNSEERLYISPPLVVSFNTQLINQVNFRLPRLE
                     NEREANHFDAAPRLELLDGDATPIPVKLDILAITSTAKTIDYEVEVERYNKSAKRASL
                     PQFATMMADDSTLLSGVSELDAIPPQSQVLTEQRLKYWFKLADPQTRNTFLQWAEKQP
                     SS"
BASE COUNT         2892 a         2676 c         2872 g         2580 t
ORIGIN      
        1 ccttgtttcc ccgttaactc acgttgctgg caaatagcaa agaagccttc gattttctca
       61 tttaaaccac cgactggctg ggcgcgaccg aactgatcga ctgaacctgt gatagcgata
      121 ctctgattca ccggcacatc ggcgagggcg ctgatcagag cgcagagttc agccatcgag
      181 gcactatcgc catccacttc actgtatgac tgctcaaatg tcagcgatgc tgagaagggg
      241 atctgttgct caagctgtag ttccgacatc aggaacgctt gcatgatcat catccctttc
      301 gcatggatat tgccgccaag ctccgctttg cgttcgatgt cggtgaattc accatcgcca
      361 atatgcacaa cgcagctaat gcgagaaggt tcgccaaaag cgcgtggatg acccggaaat
      421 tcaatgaccg aaagggcgtt aatttgcccg atgcgttcgc cttcggtttc aatcaggatt
      481 tgctcctgaa ggatctcatc ctgcatgcgt tcagcgagga aaccttcgcg ccattcacgc
      541 tgctgtagca ttaagtttag ctgctcgccg gagaaggtgt cgccatcaca cagggaggcg
      601 acctcttgac actggcggag gatccactgc gggctaagcg gaagtgtttc ttgttcacca
      661 gtgtagcgtg ctgcttcgcg gataagtacc ggccaggcat ccgctcccgg tgctggtaag
      721 tgattatgtc tggcggtaaa tgtcacccag cgacaccact ggcttactga ctccgcatcg
      781 acaatctgca gagtatcttc aaattcgcta taaatagcct gctctgaaag ctctggctcc
      841 atctcctgga aatcagccaa tgattcgcgt tcgcctacca gaatgacttt cagcttcaat
      901 ggcatcgaag gcacagagac ggggagaggg cgcgactcat caaacgcaac ccagtcaaaa
      961 cgctcgcggt taacgatatt tttcagccgc atccacagca gaggttgcgc cagcagtgta
     1021 cgcaaagaga tgatgagaat accgccattt gcctgatgca ccagaccagg ctgcagggta
     1081 atatcgccat taaactgacg caggcagcca aagagttgct ctgcttctac ccagtcggca
     1141 gcgacaactt gcgttaaagt cgcaaaatta tcatctgcac tcactgcgtg acgtaagcgg
     1201 atggtgtggc cggaaacctc atagtgaccg cccaccagtt ggcctgcatc gctttgtagc
     1261 gtacgcgcgg catcagcaat aagattcaga tactcagact cttccggggc cttcgccagc
     1321 ataaaagagg aggatgctcg cgtatgcagc aactgctcca gcgcaaattg cagtcgcggt
     1381 tgagtatcac tgaataaagg atcgttttcg tcaatcaaat gtggctgagc aaatatttcc
     1441 tgatagctat cggtatcagg aaccaggtca cgccatgcaa gtttcgtaat ggtcaaagtt
     1501 gatgtttttt agtctgttgt caaagccgcg attataccgt aaccggcact gcagcacacg
     1561 tagaaagcaa cgacaatacg cctggcatgg gcgttaaagc tcacaggatg gagattcttt
     1621 tcttcactgg cctaaaaagc tgatattctg taaagagtta cacggtaaca ttgagatcgc
     1681 tatgaaatat caacaacttg aaaatcttga aagcggttgg aaatggaagt atctggtgaa
     1741 gaagcaccgc gaaggggagt taatcacccg ttacatagaa gccagtgccg cccaggaagc
     1801 cgtcgatgag ttgctctcac tggaaaatga acccgtgctg gtaaatggtt ggattgacaa
     1861 gcatatgaat ccggaactgg tcaatcgcat gaagcagacc attcgggcca gacggaagcg
     1921 ccattttaat gcggaacacc agcatacgcg caaaaaatcg atcgatctgg aatttatcgt
     1981 ctggcaacgt ctggctggcc ttgcgcagcg tcgcggtaaa acgctttctg aaacgattgt
     2041 tcagctgatt gaagatgcgg aaaacaaaga gaaatacgcg aataaaatgt cttctttgaa
     2101 gcaggatctg caggcattgc tgggtaagga ataactgtca aaagtcagtt caatttacgc
     2161 aaggcgaaaa aaaaccccgc agcagcgggg ttttttacca gacgagaact taagcctgcg
     2221 gctgagttac aacgtctttg atgcctttaa cttcgatctc tacgcgacga tccggagcca
     2281 ggcaatcgat cagtgcagca cgctgtttca cgttgtcaca ggtgttgcca gtaaccgggt
     2341 tggattcgcc cataccacgt gcggagattt tgtctgccgg gataccttta gagatcaggt
     2401 aatcaacaac ggactgagca cgacgctcgg acagagcctg gttataagcg tcagaaccga
     2461 tgcggtcagt gtaacccaga acaactacgg aaccgtcttt cggatccagg ttgctcagct
     2521 ggctgtacag ctgatccaga gcagcctgac cttccggttt cagggttgct ttgttgaagg
     2581 tgaacagaac gtcagacttc agagtgaagt gcttggtctg tacttccggt gccggagctg
     2641 gagccggagc aactactgga gctgcttcgc cctgaccgaa acggtaggaa acacccaggc
     2701 tcagcatgcc gttgtccgga cgagtgccga tggtgtgtgc atcaccgatg ttgttggtcc
     2761 actggtattc cagacgggta gcgatttcgg gagtgatcgc atactcaaca ccgccagcga
     2821 agaccggaga aacgccggtg tcgtggtctt taaaggatgc gccaccaggt acgttagcct
     2881 tggtgtctgc acgccatacc ataccaccca gacgagtgta gatgtccaga tcgtcagtga
     2941 ttgggtaacc cagtttagcg gtcagctgaa cgccctgagc tttgtatgcg ccgttgatgt
     3001 tgtcgccttt gtacggcata cgacctaacc agtcgtaacc catttcaaag ccaacatacg
     3061 ggttaacctg gtaaccacca aaagcacctg cacccagttg gttttcgtgg gtcggaccat
     3121 tgttaggaat aaaaccagtg tcatggtact gggaccagcc cagtttagca ccagtgtacc
     3181 aggtgttatc tttcggagcg gcctgcgcta cggtagcgaa accagccagt gccactgcaa
     3241 tcgcgatagc tgtctttttc attttttgcg cctcgttatc atccaaaata cgccatgaat
     3301 atctccaacg agataacacg gttaaatcct tcaccgggga tctgctcaat attaactcta
     3361 ccgatatctt cggcttatgc cgagcacccc tggcgatgta aagtctacaa cgtagttgaa
     3421 aacttacaag tgtgaactcc gtcaggcata tgaaaaaaaa gtcttgtata aggtacgttt
     3481 aatctttttt gtcagcgaca atttacagaa gaaaatcgcg gaaaccgctt cagacaagcc
     3541 tccgcaagga aaattagtca cgactgaaag cattggctgg gcgataaaaa aagttccagg
     3601 attaatccta aatttactta atgatacaaa ttagagtgaa tttttagccc ggaaagttgt
     3661 ctcgttgcgt gagaggatgc gcttaccgga cgcataataa accccatagc gttaccttca
     3721 tttgccgcat caacaagttc agcatgctct tctgcagtca aatcatctgc caaccaaccg
     3781 atcaccacgc tgtaattgcc cgtgcgtaaa gcgcgaacca ttgactccac ggtgtggcaa
     3841 ggggagagct ggctaatctg cattactttc gttaagggta gcccagatgc ctgaacccat
     3901 tcgcgactca gtttttgttg cggtgttaac cagagttgcc agcgtgattg ctgaccgagt
     3961 tgctgtaaca atggcaacag tagaagttgc gtcatcatgg gctgatcttc gcgatagaca
     4021 acttcactga taagcccggc tgtagtgttt tccgtagaga cacgcgcaat tttacttgct
     4081 gcggatgaga acgacgaaga acgatgtgca tagcctgaag tgtacataat caatccagcc
     4141 cctgtgagtt actgtatgaa tgtacagtac atccagtgac gacaaagatc aaccctattt
     4201 tcggaaagag cctcgcaaat tttgtcgttg gtggcgggaa aacataaatt aatcttgccc
     4261 cttaagaata agttgcctat tttcgtagtt aacggatccg ttaatgtgaa tcattctttt
     4321 atgttatgat tttaaaagga attttatgaa aagcctctcc tataagcgga tctataaatc
     4381 acaagaatac ctggcaacgt tgggcacaat tgaataccga tcattgtttg gtagttacag
     4441 cctgaccgtt gacgacacgg tgtttgcgat ggtttctgat ggtgagttgt atcttcgggc
     4501 ttgtgagcaa agtgcacagt actgtgtaaa acatccgcct gtctggctga catataaaaa
     4561 gtgtggccga tccgttaccc tcaattacta tcgagttgat gaaagtctat ggcgaaatca
     4621 actgaagctg gtgcgtctgt cgaaatattc tctcgatgca gcgctgaaag agaaaagcac
     4681 gcgcaatacc cgggaaagac tgaaagattt gcccaatatg tcttttcatc tggaagcgat
     4741 tctcggggag gtggggatta aggatgtacg ggcgttacgt atacttgggg caaaaatgtg
     4801 ttggttgcga ctgcggcagc aaaactgtct ggtgacagaa aagattctgt ttatgcttga
     4861 aggggccatt atcggcattc atgaagctgc gctcccagtg gcacgccgcc aggagcttgc
     4921 agaatgggct gactctctta cgccgaaaca ggagtttcct gcggaacttg agtaatctgg
     4981 cgttgcagac gaccaatctc tggcagtaat gcaatcaata acccaacttg ttgtacgacc
     5041 agaggttctt tgctgtctgc ccgtggttca agttgctgca tccgctgttt caggccagct
     5101 aatgcctgat tgacgcgttc ttcatcagca ggttgatgat gtagcgcgtc atcaacatag
     5161 caaacggcgt catcaagaaa cgccagaatt tcaggattag ttaactgctc ccggtgagca
     5221 ccgagggctg agatatagct ggtaaacgta tggttaaggc acagcaaccg aaacgcggct
     5281 tcgcgaattt gcggggtaac gttcggttcg ctggacatat ttgataccac cgacgccagt
     5341 tcagcatcac ggttgtgtgc atcgcggcgg gcaatacgat acgccagacg gttatcacgc
     5401 ccctgatggt attgttccag tatggcatcg agataccggc agttggcctc tgtggcgcgt
     5461 tcgagcattc gcggcagatt gcgaaacttc cagtcaggcc agatgtagct cactgccgcc
     5521 cacgcaatgg cacaaccaat cagcgtatcg attacgcgag gtaacgccac ttcaaaacct
     5581 tcacccagca agttgaaaca cagtagcacc aaaagtgtga tgaacatcgt tgcatgggcg
     5641 tattgcacgt tacggaaggc aaaaaagagc acgccggtaa taaccagcag caccagctgc
     5701 ccttccaatg atggcacaaa ccacagcaca ggaataccaa ttgcgatacc taccagcgta
     5761 ccaataatcc ttaacttcag gcggtggcgc gtggcgttat agtttggctg gcagacaaac
     5821 aaacttgtca gcaagatcca atacccgtga tgcattccgg ttatctgaat gatggcgtag
     5881 ccgaagcaca acaccagcga cattcttacc gcatgacgga agagggcgga ttccggcgtg
     5941 aagtgacggc taagacgcag ccagatatca ctcaatccgt gcgggctgtc atcagcgagc
     6001 tcattttcgt cattattatg gggcagtgcc tgggcctgtt ctgattcaat tgttgccagt
     6061 tgggcatcaa tggcacgtaa attgttcagt aaaaatccca gtgttttgag taaatcagca
     6121 ggcgcgccgt tatcgcgcat ccgctccagc gcagcatcaa tatgcgtaaa agcgcgctca
     6181 aaatgcggat catgttgata aggctgacgc agcaaaatac agcgtgacag ttgctggcac
     6241 gcctggccct gcatcgacat cagccgctga aaacggaaca gcacgtcgct gtggcgaaaa
     6301 tgttcacgca atgtttgata ttgaatatga gaagagctgg cacgctcgtg aatatcctgc
     6361 gcgacaaagt aataatgcag tgtgcgacgc gttccccgtt gaccacgatc gccacgtaag
     6421 cgggtcagca gcgagagttt cgtctgattc aatgtcgcca tcagctgacc gttggcgaga
     6481 gccaaatcgt acagcggtgc ctggctttca tcttcaatat caggatcaaa catgcgcgac
     6541 ttgagctcaa gataacgcgc cagttgttca tagcaacgcg ccaggttgtc ctgcagcggg
     6601 cggaccggga acagcagatg accaataagt gtcaggacgt tgtaccagac ggcaccggcc
     6661 agaagataca tcggctgctg ataccagtgc tcatacagtg atgttcccaa catagtgtaa
     6721 atggcgatca gcaatgcacc gaaggcaatt gttgcatagc gttgacccag accgccgagc
     6781 aaaatgaagc cgctggtaga gagcgttaag ccaatcgcaa atagccaggg ccagggaaac
     6841 agcaattcta ctgaggccga ggcgataaaa aagcagaaca gcgtaatgat gaggttacgc
     6901 aaacgtcccg ccagtcggtc atcgagatcg gtcagcgccg ctgccaccat cccaagcgtc
     6961 agcggaatcg tcagttttac atcacccagc caccacggaa acgctgtggt tccacaaagc
     7021 gcaataaaaa tacgcgcgta atacagccag gcgctgttcc aggtatagcg tttgagcaaa
     7081 ggacttagca taaaggccat attagtggat tattcaaaac gacgacgtgc attggcttcg
     7141 cgcgcagctt gcgctgtttc taccgatacc acgcgacgac cgaccggcca tagtgcaata
     7201 gcggcaattt taaagttcgc aataccgaca ggaatgccaa tgatggaaat acattgtgcg
     7261 atgcccgttg caatgtgcat caggcataac caccagccaa agaaaatcag ccagaaaata
     7321 ttcaataccg taccgccagt attcagcagc acatttttgc cagccgggtt cagttcatcg
     7381 acatgaatag cttcattgcc ataaggcacc agagacagtt tagtgatctc ccagcaggat
     7441 cgtgtcagtg gtaaggtaaa aatcagcaca atactgacca gagtcgccaa cagccagccc
     7501 agagtggtgg caaatccgcc aagcacaaag ttcagaatgt tcaaaacggt acgcataaaa
     7561 cctcgcttta ctgtggtttt cagtaatggg cggcaattgt aacgtttttt tgggctggag
     7621 cacgttttct ctgacggtta cactgataag aaataatttc gtgtggatct acagagtcat
     7681 ggaactgaaa gcgacaacgc ttggaaaacg tctggcacag cacccttacg atcgggcggt
     7741 gatcctcaat gccgggatta aagtctccgg cgatcgccac gaatacctta ttcctttcaa
     7801 tcaattactg gcgattcact gtaagcgcgg tctggtatgg ggcgaactgg agtttgtact
     7861 gccggacgaa aaagtggtgc gtctgcacgg caccgaatgg ggcgagacgc agcgttttta
     7921 ccatcatctt gatgctcact ggcggcggtg gagtggcgag atgagcgaaa ttgcgtctgg
     7981 cgttttacgt cagcaactgg atttgattgc cacgcgcacc ggagaaaata aatggctgac
     8041 gcgtgagcaa acctctggta tgcagcaaca aatccgccag gctttgtcgg cgttaccatt
     8101 gccggttaac cgactggaag aattcgataa ctgccgtgag gcgtggcgta aatgtcaggc
     8161 ctggttgaaa gacattgaag gcgctcggtt gcagcataat caggcgtata ccgaagccat
     8221 gcttaccgag tatgcggatt ttttccgcca ggtcgagtct tcaccgctga atccggcgca
     8281 ggcccgggca gtcgttaatg gcgagcattc tctgttagtg ctggcaggtg caggaagcgg
     8341 aaaaacgtcg gtgctggtgg cccgtgcagg ctggttgctg gcgcgtggtg aagcgtcccc
     8401 tgagcaaatt ttattgctgg cgtttggtcg caaagccgct gaagagatgg acgagcgtat
     8461 tcgagaacga ctacataccg aagacattac cgcacgcaca tttcatgcgc tggcgctgca
     8521 tattattcag cagggcagca aaaaagttcc gatagtcagc aaactggaaa atgataccgc
     8581 tgcccgtcat gaacttttta ttgctgagtg gcgcaagcaa tgcagcgaaa agaaagcgca
     8641 ggcgaagggc tggcggcaat ggctgacgga agaaatgcag tggtcagtgc cagaaggtaa
     8701 tttctgggat gatgaaaaat tacagcgtcg tcttgcctct cgcctcgatc gctgggtaag
     8761 tctgatgcgg atgcacggtg gtgcacaggc agaaatgatt gccagtgcac ccgaagagat
     8821 tcgcgatctg ttcagtaaac gtatcaagtt gatggccccg ttattaaaag cctggaaagg
     8881 tgcgctgaag gcagaaaacg ctgtcgattt ttcgggcctt attcaccagg cgattgtgat
     8941 tctggagaaa ggtcgcttta tcagcccgtg gaagcatatt ctggttgatg aatttcagga
     9001 tatctcgccg cagcgtgcag cgttgttagc ggcattacgc aagcaaaata gtcaaacgac
     9061 gttgttcgct gttggtgatg actggcaggc gatttaccga ttcagcgggg cgcaaatgtc
     9121 gctcaccacg gctttccatg aaaactttgg tgaaggtgat cgctgtgatt tagacacgac
     9181 ctaccgtttt aacagtcgta tcggtgaggt ggcaaacagg tttattcagc agaacccagg
     9241 ccagcttaaa aagccgctaa acagcttaac caatggagac aaaaaagccg tcacattatt
     9301 ggatgagggg caacttgacg ctttgctgga taagctctct ggttatgcca aaccggaaga
     9361 gcgcattctg atcctggcgc gttaccatca catgaggcct gccagcttgg aaaaagcggc
     9421 aacacgctgg ccgaagttgc aaatcgactt tatgaccatt catgccagca aagggcaaca
     9481 ggcggattac gtcatcatcg ttggcttgca ggaggggagt gatggttttc cggctgcggc
     9541 gcgggagtcg attatggaag aggcgctact gccaccggtt gaggatttcc cggacgctga
     9601 agaacggcgg ttaatgtacg tggcgctgac ccgggcacgc catcgggtat gggcactgtt
     9661 taacaaagag aatccctctc cttttgtcga aatactgaaa aatctggatg tgccggtggc
     9721 gagaaaaccg taagaaacag gtggcgtttg ccacctgtgc aatattactt cagacggtcc
     9781 gcgagataac gctgataatc ggggatcaga atatcgaccg cgtcgttaaa atgcggtgac
     9841 tggataatga agtctgccgt tgccacgttg gtggcgaccg gaatgttcca taccgttgcc
     9901 agacgcagca aggctttcac gtcaggatcg tgcggcacgg catttagtgg gtcccagaag
     9961 aaaatcaata catcaatttt cccttctgag atcaatgcgc caacctgctg gtcacccccc
    10021 attgggccac tcaacatcgc gttgacgttc atgccggtcg cgcgggaaat taagttaccg
    10081 gtagtgcctg ttgcatacag tacgtgttgt tccagtaacg gttgatgccg ttccacccag
    10141 ctcatcagca tttgtttgca gtgatcgtgt gccaccagcg caatatgttt ccgcgaaggt
    10201 aaagtgcgag tcgtcagttc cataatgtac atccgtagtt aactttccta cagattactg
    10261 taagtactta tcgctgcaag ataaagactg aaaaagcctg cgcacaggca caaaaatctc
    10321 aggaggatgg ttgtttttcc gcccattgca ggaaagtatt tcgcgtttgt gggtcagcca
    10381 gtttaaacca atacttcagc cgttgttctg tgagcacctg agactgcggt ggaatagcat
    10441 ccagctcgga aacacccgaa agcagcgtac tgtcatctgc catcatcgtg gcaaattgcg
    10501 gtagtgaagc gcgtttcgcg gatttgttat agcgttcaac ctcgacctca taatcaatcg
    10561 tttttgccgt tgaggtaatg gcgagaatat ccagctttac cggaatcggc gtcgcatcgc
    10621 catccaacaa ttcaaggcgc ggcgcggcat caaaatggtt agcttcccgc tcattctcca
    10681 ggcgaggcag gcgaaaattg acctggttga tgagctgggt attaaaacta accaccagtg
    10741 gaggggagat atagagccgt tcttcactat tggaaagatg aatcgtcttc tcaacgcgaa
    10801 acactaactg atgcggtcca ttgtccagtt caatgctgtc cgcaccgcgc agcagagagc
    10861 tggaaacctt cttaccgtcg agtaccagaa gatcgacatc agtagaaagc cgcagcgtgg
    10921 tggcaaaaac ggataccggc aaacataatg caatcaaggt ggtcacgatg ccggttttca
    10981 tagatagctc ctgttaaaag accgcccgaa taataatgtg
//
LOCUS       DAHHTG010000133        11019 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.134, whole genome shotgun sequence.
ACCESSION   DAHHTG010000133 DAHHTG010000000
VERSION     DAHHTG010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11019)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11019)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11019
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.134"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            121..534
                     /locus_tag="NBI74_003715"
     CDS             121..534
                     /locus_tag="NBI74_003715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310694.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCD1933992.1"
                     /translation="MKKLAIAGALMLLAGCAEVENYNNVVKTPAPDWLAGYWQTKGPQ
                     RALVSPEAIGSLIVTKEGDTLDCRQWQRVIAVPGKLTLMSDDLTNVTVKRELYEVERD
                     GNTIEYDGMTMERVDRPTAECAAALDKAPLPTPLP"
     gene            complement(568..2055)
                     /gene="amyA"
                     /locus_tag="NBI74_003716"
     CDS             complement(568..2055)
                     /gene="amyA"
                     /locus_tag="NBI74_003716"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HCD1933993.1"
                     /translation="MRNPTLLQCFHWYYPEGGKLWPELAERADGFNDIGINMVWLPPA
                     YKGASGGYSVGYDSYDLFDLGEFDQKGSIPTKYGDKAQLLAAIDALKRNDIAVLLDVV
                     VNHKMGADEKEAIRVQRVNADDRTQIDEEIIECEGWTRYTFPARAGQYSQFIWDFKCF
                     SGIDHIENPDEDGIFKIVNDYTGEGWNDQVDDELGNFDYLMGENIDFRNHAVTEEIKY
                     WARWVMEQTQCDGFRLDAVKHIPAWFYKEWIEHVQEVAPKPLFIVAEYWSHEVDKLQT
                     YIDQVEGKTMLFDAPLQMKFHEASRMGRDYDMTQIFTGTLVEADPFHAVTLVANHDTQ
                     PLQALEAPVEPWFKPLAYALILLRENGVPSVFYPDLYGAHYEDVGGDGQTYPIDMPII
                     EQLDELILARQRFAHGVQTLFFDHPNCIAFSRSGTDEYPGCVVVMSNGDDGEKTIHLG
                     ENYGNKTWRDFLGNRQESVVTDKNGEATFFCNGGSVSVWVIEEVI"
     gene            complement(2133..2498)
                     /gene="fliT"
                     /locus_tag="NBI74_003717"
     CDS             complement(2133..2498)
                     /gene="fliT"
                     /locus_tag="NBI74_003717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliT"
                     /protein_id="HCD1933994.1"
                     /translation="MNNAPHLYFAWQQLVEKSQLMLRLATEEQWDELIASEMAYVNAV
                     QEIAHLTEEVAPSTTMQEQLRPMLRLILDNESKVKQLLQIRMDELAKLVGQSSVQKSV
                     LSAYGDQGGFVLAPQDNLF"
     gene            complement(2498..2908)
                     /gene="fliS"
                     /locus_tag="NBI74_003718"
     CDS             complement(2498..2908)
                     /gene="fliS"
                     /locus_tag="NBI74_003718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HCD1933995.1"
                     /translation="MYAAKGTQAYAQIGVESAVMSASQQQLVTMLFDGVLSALVRARL
                     FMQDNNQQGKGVSLSKAINIIENGLRVSLDEESKDELTQNLIALYSYMVRRLLQANLR
                     NDVSAVEEVEALMRNIADAWKESLLSPSLIQDPV"
     gene            complement(2924..4339)
                     /gene="fliD"
                     /locus_tag="NBI74_003719"
     CDS             complement(2924..4339)
                     /gene="fliD"
                     /locus_tag="NBI74_003719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HCD1933996.1"
                     /translation="MASISTLGVGSGLDLSSILDSLEAAEKSTLTPISKQQSSYTAKL
                     SAYGTLKSALESFQTANTALNKADLFTATSTTSSSSAFSATTTGSAIAGKYTISVSQL
                     AQAQTLTTKNSQKDSKAAIATSDSVLTIQQGGGKDPVTIDISAGNSSLSGIRDAINNA
                     KAGVSASIINVGNGEYRLSITANDTGSNNAMKLSVSGDSALESFMGYNGTPGDSSNGM
                     IESVTAQNAKLTVNNVEIENSSNTISDSLEDITLNLNDVTTGNQTLTISKDTSKAENA
                     VKAWVDAYNTLQDTFSSLTKYTAVDAGAESQDSSNGALLGDSTLRTIQTQLKTLLANT
                     HSSSNYKTLAQIGITSDASTGKLEIATDKLQTALKNDAAGIGEMFIGDGKSTGVTTGI
                     SNNLTSWLSSTGIIQAAKDGVSKTLNNLTDQYNAASERIDTLMTRYKAQFTQLDVLMN
                     SLNSTSSYLTQQFDTSNSNSK"
     gene            4587..5897
                     /locus_tag="NBI74_003720"
     CDS             4587..5897
                     /locus_tag="NBI74_003720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000079739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliC/FljB family flagellin"
                     /protein_id="HCD1933997.1"
                     /translation="MAQVINTNSLSLITQNNINKNQSALSTSIERLSSGLRINSAKDD
                     AAGQAIANRFTSNIKGLTQAARNANDGISLAQTTEGALSEINNNLQRVRELTVQATTG
                     TNSDSDLSSIQDEIKSRLDEIDRVSGQTQFNGVNVLAKDGSMKIQVGANDGQTISIDL
                     QKIDSSTLGLNGFSVSGQSLNVSDSITQITGAAGAKPVGVDFTAVAKDLTTATGKTVD
                     VSSLTLHNTLDAKGAATSQFVVQSGNDFYSASINHTDGKVTLNKADVEYTDTDNGLTT
                     AATQKDQLIKVAADSDGSAAGYVTFQGKNYATTVSTALDDNTAAKATDNKVVVELSTA
                     KPTAQFSGASSADPLALLDKAIAQVDTFRSSLGAVQNRLDSAVTNLNNTTTNLSEAQS
                     RIQDADYATEVSNMSKAQIIQQAGNSVLSKANQVPQQVLSLLQG"
     gene            6216..6935
                     /gene="fliA"
                     /locus_tag="NBI74_003721"
     CDS             6216..6935
                     /gene="fliA"
                     /locus_tag="NBI74_003721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FliA"
                     /protein_id="HCD1933998.1"
                     /translation="MNSLYTAEGVMDKHSLWQRYVPLVRHEALRLQVRLPASVELDDL
                     LQAGGIGLLNAVERYDALQGTAFTTYAVQRIRGAMLDELRSRDWVPRSVRRNAREVAQ
                     AIGQLEQELGRNATETEVAERLGIDIADYRQMLLDTNNSQLFSYDEWREEHGDSIELV
                     TDDHQRENPLQQLLDNNLRQRVMEAIETLPEREKLVLTLYYQEELNLKEIGAVLEVGE
                     SRVSQLHSQAIKRLRTKLGKL"
     gene            6981..7532
                     /gene="fliZ"
                     /locus_tag="NBI74_003722"
     CDS             6981..7532
                     /gene="fliZ"
                     /locus_tag="NBI74_003722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310687.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliZ"
                     /protein_id="HCD1933999.1"
                     /translation="MMVQHLKRRPLSRYLKDFKHSQTHCAHCRKLLDRITLVRDGKIV
                     NKIEISRLDTLLDENGWQVEQQSWAALCRFCGDLHCKTQSDFFDIIGFKQFLFEQTEM
                     SPGTVREYVVRLRRLGNHLHEQNISLDQLQDGFLDEILAPWLPSTSTSNYRIALRKYQ
                     HYQRQTCTGLVQKSSSLPASDIY"
     gene            7620..8420
                     /gene="tcyJ"
                     /locus_tag="NBI74_003723"
     CDS             7620..8420
                     /gene="tcyJ"
                     /locus_tag="NBI74_003723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter substrate-binding
                     protein"
                     /protein_id="HCD1934000.1"
                     /translation="MKLAHLGRQALMGVMAVALVAGMSVKSFADEGLLNKVKERGTLL
                     VGLEGTYPPFSFQGDDGKLTGFEVEFAQQLAKHLGVEASLKPTKWDGMLASLDSKRID
                     VVINQVTISDERKKKYDFSTPYTISGIQALVKKGNEGTIKTADDLKGKKVGVGLGTNY
                     EEWLRQNVQGVDVRTYDDDPTKYQDLRVGRIDAILVDRLAALDLVKKTNDTLAVTGEA
                     FSRQESGVALRKGNEDLLKAVNDAIAEMQKDGTLQALSEKWFGADVTK"
     gene            8525..9511
                     /gene="dcyD"
                     /locus_tag="NBI74_003724"
     CDS             8525..9511
                     /gene="dcyD"
                     /locus_tag="NBI74_003724"
                     /EC_number="4.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-cysteine desulfhydrase"
                     /protein_id="HCD1934001.1"
                     /translation="MPLHNLTRFPRLEFIGAPTPLEYLPRFSDYLGREIFIKRDDVTP
                     MAMGGNKLRKLEFLAADALREGADTLITAGAIQSNHVRQTAAVAAKLGLHCVALLENP
                     IGTTAENYLTNGNRLLLDLFNTQIEMCDALTDPNAQLEELATRVEAQGFRPYVIPVGG
                     SNALGALGYVESALEIAQQCEGAVNISSVVVASGSAGTHAGLAVGLEHLMPESELIGV
                     TVSRSVADQLPKVVNLQQAIAKELELTASAEILLWDDYFAPGYGVPNDEGMEAVKLLA
                     RLEGILLDPVYTGKAMAGLIDGISQKRFKDEGPILFIHTGGAPALFAYHPHV"
     gene            9526..10194
                     /gene="tcyL"
                     /locus_tag="NBI74_003725"
     CDS             9526..10194
                     /gene="tcyL"
                     /locus_tag="NBI74_003725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter permease"
                     /protein_id="HCD1934002.1"
                     /translation="MQESIQLVIDSLPFLLKGAGYTLQLSIGGMFFGLLLGFILALMR
                     LSPIWPVRWLARFYISIFRGTPLIAQLFMIYYGLPQFGIELDPIPSAMIGLSLNTAAY
                     AAETLRAAISSIDKGQWEAAASIGMTPWQTMRRAILPQAARVALPPLSNSFISLVKDT
                     SLAATIQVPELFRQAQLITSRTLEVFTMYLAASLIYWIMATVLSTLQNHFENQLNRQE
                     REPK"
     gene            10191..10944
                     /gene="yecC"
                     /locus_tag="NBI74_003726"
                     /pseudo
     CDS             10191..10944
                     /gene="yecC"
                     /locus_tag="NBI74_003726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416427.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine ABC transporter ATP-binding protein
                     YecC"
BASE COUNT         2672 a         2701 c         2864 g         2782 t
ORIGIN      
        1 tttcggggta ataattcaca aaaataatgg ttagatcaaa tttctctttg aacctggaaa
       61 ggctggcgac atcagggctt taatgccaca atcctctttt cttctcatgc aggacgaatg
      121 atgaaaaaac tcgcaattgc aggcgcgctc atgttgctgg cgggctgtgc cgaagtcgaa
      181 aactataaca atgtcgtgaa aacccccgcg ccggactggc tggcgggcta ctggcagacc
      241 aaagggccgc aacgtgcgtt ggtcagtccg gaagcgatag gcagcctgat tgtcactaaa
      301 gagggcgata ccctggattg ccgtcagtgg cagcgggtga ttgccgtgcc gggtaagttg
      361 acattgatgt ctgatgatct cactaacgtt accgtaaaac gagagctgta cgaggttgag
      421 cgtgacggta acaccattga gtacgatggt atgacgatgg agcgtgtgga tcgcccgacg
      481 gcagagtgtg ccgccgcgct ggataaagcg ccgttaccga cgccactgcc gtaatgaaat
      541 ctccccggct tgccgccggg gatggattca aatcacctct tcgataaccc acacgctaac
      601 gctgccgccg ttgcaaaaga aggtcgcttc gccgtttttg tctgtcacta cgctctcttg
      661 ccggttcccc agaaaatcac gccaggtttt attgccgtaa ttctctccca gatgaatggt
      721 tttttcgcca tcatctccgt tcgacatgac caccacgcag ccgggatatt cgtcggtgcc
      781 actgcggcta aaggcaatgc agttcggatg gtcgaaaaat aacgtctgta caccgtgggc
      841 gaaacgctga cgggcgagaa ttaactcatc aagctgttcg attattggca tatctatcgg
      901 ataggtttgc ccgtcaccac cgacatcttc gtaatgtgca ccgtagaggt ccggatagaa
      961 caccgaagga acgccatttt cccgcaacaa aattaaggca tacgccagcg gtttaaacca
     1021 cggttcgacg ggggcttcga gggcttgcaa cggttgggtg tcgtggttgg caacgagggt
     1081 tacggcgtga aaaggatcgg cttccaccag cgtcccagtg aagatctgcg tcatgtcgta
     1141 gtcgcgaccc atgcgcgatg cttcatggaa tttcatctgc agcggcgcat caaacagcat
     1201 ggttttgcct tccacctgat caatatacgt ttgcagttta tcgacttcat gcgaccagta
     1261 ttcagccaca ataaacaacg gctttggcgc aacttcctgt acgtgttcga tccactcttt
     1321 ataaaaccat gccggaatat gtttgaccgc atcaagacga aagccgtcgc attgcgtttg
     1381 ttccatcacc cagcgcgccc agtatttaat ctcttccgtc acggcatgat tgcgaaaatc
     1441 gatattctcc cccatcagat aatcgaaatt accgagttca tcatcaacct gatcgttcca
     1501 gccttctccg gtatagtcgt taacaatttt aaaaatgcca tcttcatcag ggttttcgat
     1561 atggtcgata ccgctaaaac atttgaaatc ccagataaac tgcgagtatt gcccggcacg
     1621 agcggggaag gtgtaacgcg tccagccttc acactcaatg atttcttcgt caatttgcgt
     1681 acggtcatca gcatttacac gctgcacgcg aatagcttct ttttcatccg cgcccatttt
     1741 gtggttgact accacgtcca gcagcaccgc aatgtcatta cgtttcaggg cgtcaatggc
     1801 ggccagcagt tgtgctttat caccatattt agtagggatg ctgcctttct gatcaaactc
     1861 acctagatca aataaatcat aggagtcgta gccgaccgaa tagccgcccg atgcgccttt
     1921 ataggcgggc ggcaaccaga ccatattgat accaatatca ttaaaaccgt ctgcgcgctc
     1981 ggccagttca ggccagagct taccgccttc cgggtaatac cagtgaaaac attgtaacag
     2041 cgtgggatta cgcattcttc gactccattc aaggggaata ttagaagcgt agccgtaatc
     2101 ggattattcg cgagccatcg gctcattaag attcaaaaga ggttatcctg cggagccagc
     2161 acaaagccgc cctgatcgcc ataggcactt aacaccgatt tttgcaccga tgactgaccg
     2221 accagtttcg ccagttcatc catccgaatc tgtaataact gttttacctt gctttcgttg
     2281 tcgagaatca ggcgcagcat cgggcggagc tgctcctgca tcgtagtgga cggggcaacc
     2341 tcttcagtca ggtgtgcgat ctcctgcacc gcattcacat acgccatttc gctggcgatg
     2401 agttcgtccc attgttcttc cgttgccagg cgtaacatga gctggctttt ttcgacgagt
     2461 tgttgccagg cgaaatacaa atgcggtgca ttgttcatca gactgggtcc tgaatcaaag
     2521 aaggggagag taaagactct ttccaggcat cggcaatatt gcgcattaat gcttccactt
     2581 cttcgactgc ggagacatcg ttacgtaaat tggcttgcag caagcgcctg accatatagc
     2641 tataaagagc aatcaagttt tgggttaatt cgtctttgct ctcttcatca agactcaccc
     2701 gcagtccgtt ctcaatgatg ttgatcgctt ttgataaaga gacgcctttg ccttgctgat
     2761 tgttgtcctg cataaacagg cgcgctctaa ccagtgcgct cagcactcca tcaaatagca
     2821 tggtgaccag ctgctgttgg ctggcgctca ttacggcgct ttcgacgcca atttgtgcat
     2881 aggcctgggt gccttttgcc gcgtacatag tctctcctga agattatttc gagttgctat
     2941 ttgaggtgtc gaactgctgt gtcagatagc tactagtgga gttcagcgag ttcattaaca
     3001 catctaattg ggtgaactgg gctttatagc gcgtcattaa ggtatcgata cgttcactgg
     3061 ctgcgttgta ctggtcggtc aaattattca gcgttttgct gacgccatct tttgctgcct
     3121 ggattatccc ggtggaagag agccaactgg tcaggttgtt actaatgccg gtggttaccc
     3181 cagtactttt gccgtcacct ataaacattt caccgatacc agcggcatca ttttttagcg
     3241 ccgtttgcag tttgtcggtc gcgatttcca gcttgccggt actggcatcg ctggtaatgc
     3301 caatctgtgc cagcgtttta tagtttgaac tgctgtgagt attggcgagc aaggttttaa
     3361 gctgagtctg gatggtacgc agtgtggagt caccgagtaa tgcgccatta ctggagtcct
     3421 gactttcagc tccggcatcg acagcggtgt atttggttaa gctgctgaat gtatcctgca
     3481 atgtgttata ggcatccacc cacgctttaa ccgcattttc agctttggat gtgtctttac
     3541 tgatggtcag tgtctgatta ccggtggtga cgtcattcag gttgagggtg atatcttcca
     3601 gtgagtcgct gatagtgtta ctgctgtttt caatctcaac gttattgact gtcagtttgg
     3661 cgttttgcgc ggtgacgctt tcaatcatgc cattgctgct atcacccggc gttccgttgt
     3721 aacccataaa actttccagg gcgctgtcgc cgctgacgct aagtttcata gcgttattgc
     3781 tgcccgtatc gttagcggtg atggacagac gatattcgcc gtttcccaca ttgatgatac
     3841 tggcgctgac accagctttg gcgttgttaa tggcatcgcg aatgccggaa agtgacgagt
     3901 tacccgcgct gatatcaatc gtgaccggat ctttaccgcc gccttgttgg atggtcagta
     3961 cgctatcgct ggtggcgatg gcggctttac tgtctttttg actatttttt gttgtcagcg
     4021 tctgtgcctg cgccagttgg ctaacgctaa tggtgtattt cccagcaatc gcgctgcccg
     4081 tggtggtggc gctgaaagcc gatgaactgc tggtggttga ggtggcggta aataaatcgg
     4141 ctttattcag tgctgtgttg gctgtctgga aactctctag tgcacttttc agcgtcccgt
     4201 aggcgctcag tttcgcggtg taagagcttt gctgttttga tatcggcgtc agggtagatt
     4261 tttctgccgc ctccagactg tcaaggatgc tgcttaagtc cagccccgac ccaaccccaa
     4321 gcgttgaaat actagccata tagtttcctt aatcatgcga cgtgtataga acgaataccg
     4381 gggttatcgg cgtaagcggg gtaaagttta cgatttattt tttgccttaa tggcacgaac
     4441 agcaacgagg aaggggagta tttcgaccgc tagaaaaaaa ttctaaaggt tgtgagtgac
     4501 cagacgataa cagggttgac ggcgacgaag ccgaagggtg gaagcccaat acttaaaccg
     4561 tagacttgaa aacaggaaaa tgaattatgg cacaagtcat taataccaac agcctctcgc
     4621 tgatcactca gaacaacatc aacaaaaacc agtctgcgct gtcgacttct atcgagcgcc
     4681 tctcttctgg tctgcgtatt aacagcgcta aagatgacgc cgcgggccag gcgattgcta
     4741 accgctttac ttctaacatc aaaggtctga ctcaggccgc acgtaacgcc aacgacggta
     4801 tttctctggc gcagaccact gaaggcgcgc tgtctgaaat caacaacaac ttgcagcgtg
     4861 tgcgtgagtt gaccgttcaa gcgacgaccg ggactaactc tgattctgac ctgtcttcaa
     4921 tccaggacga aatcaaatcc cgtctggatg aaattgatcg cgtttccggt cagacccagt
     4981 tcaacggcgt gaatgtgctg gcgaaagatg gttcgatgaa gattcaggtt ggcgcgaatg
     5041 atgggcagac tattagcatt gatttgcaga agattgactc ttctacatta ggactgaacg
     5101 gtttctccgt ttcgggtcag tcacttaacg ttagtgattc cattactcaa attaccggtg
     5161 ccgccggggc aaaacctgtt ggtgttgatt tcactgctgt tgcgaaagat ctgactactg
     5221 cgacaggtaa aacagtcgat gtttctagcc tgacgttaca caacactctg gatgcgaaag
     5281 gggctgctac atcacagttc gtcgttcaat ccggcaatga tttctactcc gcgtcgatta
     5341 atcatacaga cggcaaagtc acgttgaata aagccgatgt cgaatacaca gacaccgata
     5401 atggactaac gactgcggct actcagaaag atcaactgat taaagttgcc gctgactctg
     5461 acggctcggc tgcgggatat gtaacattcc aaggtaaaaa ctacgctaca acggtttcaa
     5521 cggcacttga tgataatact gcggcaaaag caacagataa taaagttgtt gttgaattat
     5581 caacagcaaa accgactgca cagttctcag gggcttcttc tgctgatcca ctggcacttt
     5641 tagacaaagc gattgcacag gttgatactt tccgctcctc cctcggtgcg gtgcaaaacc
     5701 gtctggattc cgcagtcacc aacctgaaca acaccaccac caacctgtct gaagcgcagt
     5761 cccgtattca ggacgccgac tatgcgaccg aagtgtccaa catgtcgaaa gcgcagatta
     5821 tccagcaggc aggtaactcg gtgctgtcca aagctaacca ggttccgcag caggttctgt
     5881 ctctgctgca gggttaatcg ccgtaacctg attaactgag actgacggca acgccaaatt
     5941 gccttatgcg ctgcgcttat caggcctaca aggtgaaatg caatttattg aatttgcaga
     6001 tttttgtagg ccggataagg cgttacgccg catccggcaa catgaatggt aatttgtcag
     6061 caacgtgctt ccccgccaac ggcggggttt tttctgcccg caatttaccg gtaaccccca
     6121 aataacccct catttcaccc actaatcgtc cgattaaaaa ccctgcagaa acggataatc
     6181 atgccgataa ctcatataac gcagggctgt ttatcgtgaa ttcactctat accgctgaag
     6241 gtgtaatgga taaacactcg ctgtggcagc gttatgtccc gctggtgcgt cacgaagcat
     6301 tgcgcctgca ggttcgactg cccgcgagcg tggaacttga cgatctgcta caggcgggcg
     6361 gcattgggtt acttaatgcc gtcgaacgct atgacgccct acaaggaacg gcatttacaa
     6421 cttacgcagt gcagcgtatc cgtggcgcta tgctggatga acttcgcagc cgtgactggg
     6481 tgccgcgcag cgtgcgacgc aacgcgcgtg aagtggcaca ggcaataggg caactggagc
     6541 aggaacttgg ccgcaacgcc acggaaactg aggttgcgga acgtttaggg atcgatattg
     6601 ccgattatcg ccaaatgttg ctcgacacca ataacagcca gctcttctcc tacgatgagt
     6661 ggcgtgaaga gcacggcgat agcatcgaac tggttactga tgatcatcag cgagaaaacc
     6721 cgctacaaca actgctggac aataatctgc gccagcgggt gatggaagcc atcgaaacgt
     6781 tgccggagcg cgaaaaactg gtattaaccc tctattatca ggaagagctg aatctcaaag
     6841 agattggtgc ggtgctggag gtcggggaat cgcgggtcag tcagttacac agccaggcta
     6901 ttaaacggtt acgcactaaa ctgggtaagt tataacgtca gtaaatgccg cactttaatt
     6961 ttgactacca ggagttctta atgatggtgc agcacctgaa aagacggcca ttaagccgct
     7021 atcttaaaga ctttaaacac agccagaccc attgcgcgca ttgccgtaaa ttactcgatc
     7081 gcattaccct cgtccgcgac ggcaaaatag tgaataaaat cgagatttcc cgactggaca
     7141 cgctgcttga tgaaaatggc tggcaagtgg aacaacaatc atgggcggca ttgtgccgat
     7201 tttgcggtga tttacattgc aaaacgcaga gtgatttttt cgatattatc ggctttaagc
     7261 aatttctttt tgagcaaact gaaatgagcc ctggtacggt gcgtgaatat gtcgttcgtt
     7321 tgcgccgttt ggggaatcat ctacacgagc aaaatatttc cctcgatcag ctgcaggacg
     7381 gtttccttga tgaaatcctc gccccgtggc tgccctccac cagcaccagc aattaccgca
     7441 tcgcgttacg gaagtatcaa cactatcagc gtcaaacgtg taccggactt gtgcagaaat
     7501 cctccagcct gcctgcttct gatatatatt aaataagaat aagatgtagc ggagttgttt
     7561 ttgtgtttac aaacaatggc tctacactgc aaacagacat aacaacattc ggggtgaata
     7621 tgaaattagc acatctggga cgtcaggcat tgatgggtgt gatggccgtg gcgctggttg
     7681 cgggtatgag cgttaaaagt tttgcagatg aaggtctgct taataaagtc aaagagcgcg
     7741 gcacgctgct ggtagggctg gagggaactt atccgccgtt cagttttcag ggggatgacg
     7801 gcaaattaac cggttttgaa gtggaatttg cccaacaact ggcaaaacat cttggcgttg
     7861 aggcgtcact aaaaccgacc aaatgggacg gtatgctggc gtcgctggac tctaaacgca
     7921 ttgatgtggt gattaaccag gtcaccattt ctgacgagcg caagaaaaaa tacgatttct
     7981 ctaccccgta caccatttct ggtattcagg cgctggtgaa aaaaggcaac gaaggcacca
     8041 ttaaaacggc tgatgatctg aaaggcaaaa aagtgggtgt cggtctgggc accaactatg
     8101 aagagtggct gcgtcagaat gttcagggcg tcgatgtgcg tacctatgat gatgacccga
     8161 ccaaatatca ggatctgcgc gtaggccgta tcgatgcgat cctcgttgat cgtctggcgg
     8221 cgctggatct ggtgaagaaa accaacgata cgctggcagt aaccggtgaa gcattctctc
     8281 gtcaggagtc tggcgtggcg ctgcgtaaag gaaatgaaga cctgctgaaa gcggtgaatg
     8341 atgcaattgc ggaaatgcaa aaagatggca ctttgcaagc cctttccgaa aaatggtttg
     8401 gtgctgatgt gaccaaataa tcagcataat gacaaaaaag ggcgctttca ctagcgcctt
     8461 ttttatttac gcgttttcag cgtgcataat aagaaaatca caacaacaca catcggaggc
     8521 tgttatgcca ctgcataatt taacccgttt tccacggctg gagtttatcg gcgcgccaac
     8581 gccgctcgaa tatctgccgc gcttttctga ttatcttgga cgggaaattt tcatcaaacg
     8641 ggatgacgtc actcccatgg caatgggcgg caataaatta cgtaagctgg aatttctcgc
     8701 ggcagatgct ctgcgtgaag gtgccgatac gctgattact gccggggcga tccagtctaa
     8761 ccatgtgcgc cagactgccg cagtcgctgc gaaactcggt ctgcactgcg tggcgctgct
     8821 ggaaaatcct attggtacaa ccgcggaaaa ctatttaacc aacggcaatc gtttgttgct
     8881 ggatctgttc aatacccaga ttgaaatgtg cgacgcactg accgatccca atgcccaact
     8941 ggaagagctg gcgacgcgag tcgaagcaca aggctttcgc ccgtatgtca ttccggttgg
     9001 cggttctaat gctttgggcg cgctgggtta tgtggagagt gcgctggaaa tcgcgcaaca
     9061 gtgtgaaggg gcggttaata tttcgtcggt ggtggtcgca tcgggcagtg ccggaactca
     9121 cgccggactg gctgttgggc tggaacacct gatgcctgaa agcgaactga ttggcgtgac
     9181 tgtgtcgcgt tctgttgccg atcaattgcc gaaagtggtt aacctccaac aggcgattgc
     9241 gaaagaactg gagctgaccg catcagcgga aattttactc tgggatgact attttgcacc
     9301 tggctacggc gtgccgaacg acgaaggcat ggaagcagtg aaattgctgg cgaggctgga
     9361 aggcattctg cttgatcctg tgtataccgg aaaagcgatg gcggggctga ttgacggtat
     9421 cagtcagaaa cgcttcaaag atgaagggcc gattttgttt attcataccg gcggtgcgcc
     9481 tgcgctgttc gcctatcatc cccacgttta gctccgggaa cgctcatgca agaaagtata
     9541 caactggtta ttgattcctt gccgttcctg ctgaaagggg ctgggtatac gctgcaactc
     9601 agtatcggag gcatgttttt tggcttactg ctcgggttta ttctcgcgct gatgcgcctg
     9661 tcgccaatct ggcccgtgcg ctggctggcg cgtttttata tctctatttt tcgtggtacg
     9721 ccgctcatcg cacaactgtt tatgatctat tacggactgc cacagtttgg tatcgaatta
     9781 gatcccattc cgtcagcaat gattggtctg tcgctgaaca ctgccgccta tgctgccgaa
     9841 acgctgcgcg ccgccatttc ttctattgat aaaggtcagt gggaagctgc agccagtatt
     9901 ggtatgacgc catggcagac catgcgtcgc gcgattttgc cacaggcggc gcgggtggcg
     9961 ttgccgccgc tgtcgaacag ctttatcagc ctggtaaaag atacgtcgct ggccgcgaca
    10021 atccaggtgc cggagctgtt ccgtcaggcg cagttgatta cctcgcgcac gctggaggtg
    10081 ttcaccatgt acctggcggc ttcgctcatc tactggatca tggcgactgt gttatcgacg
    10141 ttgcagaacc attttgagaa tcaacttaat cgccaggaga gagaacccaa atgagtgcca
    10201 ttgaagttaa gaacctggtg aaaaaattcc acggtcagac ggtgctgcat ggtatcgacc
    10261 ttgaggtgaa gcctggcgaa gtagtggcaa ttatcggtcc gagtggttcc ggcaaaacca
    10321 cgttgctacg cagcataaat ctgctggaac aaccggaagc gggaaccatc accgttggcg
    10381 atatcactat tgatactgca cgttcattaa gtcagcaaaa atcgctgatt cgccagttgc
    10441 gtcagcacgt cgggtttgtc ttccagaact ttaatctgtt tccgcatcgt acggtgctgg
    10501 aaaacattat tgaagggccg gtgatcgtca aaggcgaacc gaaagaagag gccacggcgc
    10561 gcgctcgcga gctgctggcg aaagttgggc tggccggtaa agaaaccagt tatccacgtc
    10621 gtttgtctgg cggtcaacag cagcgcgttg cgattgcgcg tgctctggca atgcgtcctg
    10681 aagtgatttt gtttgatgag ccaacgtcgg cgctggatcc agagctggtg ggcgaagtcc
    10741 tgaacaccat tcgtcagctg gcgcaggaaa agcgcacgat ggtgattgtg acgcacgaaa
    10801 tgagctttgc ccgggatgtt gcggaccggg cgatttttat ggaccagggg cggatagtcg
    10861 agcagggggg tagcaaaagc gttatttgct gatcctcagc agcctcgcac ccgccagttc
    10921 ctggagaagt ttctgctgca ataataagaa aaaatcagcc ccgacgattc accagtcggg
    10981 gcgggacgcc atttcaaacg tgataaaact gcttaacaa
//
LOCUS       DAHHTG010000134        10996 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.135, whole genome shotgun sequence.
ACCESSION   DAHHTG010000134 DAHHTG010000000
VERSION     DAHHTG010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10996)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10996)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10996
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.135"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            95..748
                     /gene="ompW"
                     /locus_tag="NBI74_003727"
     CDS             95..748
                     /gene="ompW"
                     /locus_tag="NBI74_003727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpW"
                     /protein_id="HCD1934003.1"
                     /translation="MKKLTVAALAVATLLSGSAFAHEAGEFFMRAGSATVRPTEGAGG
                     TLGSLGGFSVTNNTQLGLTFTYMATDNIGVELLAASPFRHKVGTATTGDIATVHHLPP
                     TLMAQWYFGDSGSKFRPYIGAGINYTTFFNNDFNDTGEGLGLSDLSLKDSWGVAGQVG
                     LDYLINRDWLLNMSVWYMDIDTEVKYKSNGAVLPAGRYSDNVRLDPWVFMFSAGYRF"
     gene            complement(799..1605)
                     /gene="trpA"
                     /locus_tag="NBI74_003728"
     CDS             complement(799..1605)
                     /gene="trpA"
                     /locus_tag="NBI74_003728"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000443083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HCD1934004.1"
                     /translation="MERYESLFAQLKERKEGAFVPFVTLGDPGIEQSLKIIDTLIEAG
                     ADALELGIPFSDPLADGPTIQNATLRAFAAGVTPAQCFEMLALIRQKHPTIPIGLLMY
                     ANLVFNKGIDEFYAECEKVGVDSVLVADVPVEESAPFRQAALRHNVAPIFICPPNADD
                     DLLRQIASYGRGYTYLLSRAGVTGAENRAALPLNHLVAKLKEYNAAPPLQGFGISAPD
                     QVKAAIDAGTAGAISGSAIVKIIEQHINEPEKMLAALKAFVQPMKTATRR"
     gene            complement(1605..2798)
                     /gene="trpB"
                     /locus_tag="NBI74_003729"
     CDS             complement(1605..2798)
                     /gene="trpB"
                     /locus_tag="NBI74_003729"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HCD1934005.1"
                     /translation="MTTLLNPYFGEFGGMYVPQILMPALRQLEEAFVSAQKDPEFQAQ
                     FNDLLKNYAGRPTALTKCQNITAGTNTTLYLKREDLLHGGAHKTNQVLGQALLAKRMG
                     KTEIIAETGAGQHGVASALASALLGLKCRIYMGAKDVERQSPNVFRMRLMGAEVIPVH
                     SGSATLKDACNEALRDWSGSYETAHYMLGTAAGPHPYPTIVREFQRMIGEETKAQILE
                     REGRLPDAVIACVGGGSNAIGMFADFINETDVGLIGVEPGGHGIETGEHGAPLKHGRV
                     GIYFGMKAPMMQTEDGQIEESYSISAGLDFPSVGPQHAYLNSTGRADYVSITDDEALE
                     AFKTLCLHEGIIPALESSHALAHALKMMRENPEKEQLLVVNLSGRGDKDIFTVHDILK
                     ARGEI"
     gene            complement(2810..4168)
                     /gene="trpCF"
                     /locus_tag="NBI74_003730"
     CDS             complement(2810..4168)
                     /gene="trpCF"
                     /locus_tag="NBI74_003730"
                     /EC_number="4.1.1.48"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional indole-3-glycerol-phosphate
                     synthase TrpC/phosphoribosylanthranilate isomerase TrpF"
                     /protein_id="HCD1934006.1"
                     /translation="MQTVLAKIVADKAIWVEARKQQQPLASFQNEIQPSTRHFYDALQ
                     GARTAFILECKKASPSKGVIRDDFDPARIAAIYKHYASAISVLTDEKYFQGSFDFLPI
                     VSQIAPQPILCKDFIIDPYQIYLARYYQADACLLMLSVLDDEQYRQLAAVAHSLEMGV
                     LTEVSNEEELERAIALGAKVVGINNRDLRDLSIDLNRTRELAPKLGHNVTVISESGIN
                     TYAQVRELSHFANGFLIGSALMAHDDLNAAVRRVLLGENKVCGLTRGQDAKAAYDAGA
                     IYGGLIFVATSPRCVNVEQAQEVMAAAPLQYVGVFRNHDIADVVDKAKVLSLAAVQLH
                     GNEDQLYIDTLREALPAHVAIWKALSVGETLPARDFQHIDKYVFDNGQGGSGQRFDWS
                     LLNGQSLGNVLLAGGLGADNCVEAAQTGCAGLDFNSAVESQPGIKDARLLASVFQTLR
                     AY"
     gene            complement(4172..5767)
                     /gene="trpD"
                     /locus_tag="NBI74_003731"
     CDS             complement(4172..5767)
                     /gene="trpD"
                     /locus_tag="NBI74_003731"
                     /EC_number="2.4.2.18"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008784940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional anthranilate synthase glutamate
                     amidotransferase component TrpG/anthranilate
                     phosphoribosyltransferase TrpD"
                     /protein_id="HCD1934007.1"
                     /translation="MADILLLDNIDSFTYNLADQLRSNGHNVVIYRNHIPAQTLIERL
                     ATMSNPVLMLSPGPGVPSEAGCMPELLTRLRGKLPIIGICLGHQAIVEAYGGYVGQAG
                     EILHGKASSIEHDGQAMFAGLTNPLPVARYHSLVGSNIPAGLTINAHFNGMVMAVRHD
                     ADRVCGFQFHPESILTTQGARLLEQTLAWAQQKLEQTNTLQPILEKLYQAQTLSQQES
                     HQLFSAVVRGELKPEQLAAALVSMKIRGEHPNEIAGAATALLENAAPFPRPDYLFADI
                     VGTGGDGSNSINISTASAFVAAACGLKVAKHGNRSVSSKSGSSDLLAAFGINLDMNAD
                     KSRQALDELGVCFLFAPKYHTGFRHAMPVRQQLKTRTLFNVLGPLINPAHPPLALIGV
                     YSPELVLPIAETLRVLGYQRAAVVHSGGMDEVSLHAPTIVAELHDGEIKSYQLTAEDF
                     GLTPYHQEQLAGGTPEENRDILTRLLQGKGDAAHEAAVAANVAMLMRLHGHEDLQANA
                     QTVLEVLRSGSAYDRVTALAARG"
     gene            complement(5767..7329)
                     /gene="trpE"
                     /locus_tag="NBI74_003732"
     CDS             complement(5767..7329)
                     /gene="trpE"
                     /locus_tag="NBI74_003732"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HCD1934008.1"
                     /translation="MQTQKPTLELLTCVGAYRDNPTALFHQLCGDRPATLLLESADID
                     SKDDLKSLLLVDSALRITALGDTVTIQALSGNGEALLALLDNALPAGVENEQSPNCRV
                     LRFPPVSPLLDEDARLCSLSVFDAFRLLQNLLNVPKEEREAMFFGGLFSYDLVAGFED
                     LPQLSAENNCPDFCFYLAETLMVIDHQKKSTRIQASLFAPNEEEKQRLTARLNELRQQ
                     LTEAAPPLPVVSVPHMRCECNQSDEEFGGVVRLLQKAIRAGEIFQVVPSRRFSLPCPS
                     PLAAYYVLKKSNPSPYMFFMQDNDFTLFGASPESSLKYDATSRQIEIYPIAGTRPRGR
                     RADGSLDRDLDSRIELEMRTDHKELSEHLMLVDLARNDLARICTPGSRYVADLTKVDR
                     YSYVMHLVSRVVGELRHDLDALHAYRACMNMGTLSGAPKVRAMQLIAEAEGRRRGSYG
                     GAVGYFTAHGDLDTCIVIRSALVENGVATVQAGAGVVLDSVPQSEADETRNKARAVLR
                     AIATAHHAQETF"
     misc_feature    complement(7351..7444)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00513"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Trp leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00513"
     gene            complement(7421..7465)
                     /gene="trpL"
                     /locus_tag="NBI74_003733"
     CDS             complement(7421..7465)
                     /gene="trpL"
                     /locus_tag="NBI74_003733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon leader peptide"
                     /protein_id="HCD1934009.1"
                     /translation="MKAIFVLKGWWRTS"
     gene            7603..8484
                     /gene="yciV"
                     /locus_tag="NBI74_003734"
     CDS             7603..8484
                     /gene="yciV"
                     /locus_tag="NBI74_003734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exoribonuclease"
                     /protein_id="HCD1934010.1"
                     /translation="MSDTNYSVIYDLHSHTTASDGCLTPEALVHRAVEMRVGTLAITD
                     HDTTAAIAPAREEISRSGLALNLIPGVEISTVWENHEIHIVGLNIDITHPLMCEFLAQ
                     QTERRNQRAQLIAERLEKAQIPGALEGAQQLAQGGAVTRGHFARFLVECGKASSMADV
                     FKKYLARGKTGYVPPQWCTIEQAIDVIHHSGGKAVLAHPGRYNLSAKWLKRLVAHFAE
                     HHGDAMEVAQCQQSPNERTQLATLARQHHLWASQGSDFHQPCPWIELGRKLWLPAGVE
                     GVWQLWEQPQNTTEREL"
     gene            8481..9101
                     /locus_tag="NBI74_003735"
     CDS             8481..9101
                     /locus_tag="NBI74_003735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HCD1934011.1"
                     /translation="MSQFFYIHPDNPQQRLINQAVEIVRKGGVIVYPTDSGYALGCKI
                     EDKNAMERICRIRQLPDGHNFTLMCRDLSELSTYSFVDNVAFRLMKNNTPGNYTFILK
                     GTKEVPRRLLQEKRKTIGMRVPSNPIAQALLEALGEPMLSTSLMLPGSEFTESDPEEI
                     KDRLEKQVDLIIHGGYLGQKPTTVIDLTDDTPVVVREGVGDVKPFL"
     gene            9129..>10996
                     /locus_tag="NBI74_003736"
     CDS             9129..>10996
                     /locus_tag="NBI74_003736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HCD1934012.1"
                     /translation="MAGIYRCILLLIVGLFFSSLSYAKNTEIPSYEEGISLFDVEATL
                     QPNGVLDIKENIHFQARNQQIKHGFYRDLPRLWMQPDGDAALLNYHIVGVTRDGIPEP
                     WHLDWHIGLMSIVVGDKQRFLPQGDYHYQIHYQVKNAFLREGDSALLIWNVTGNHWPF
                     EIYKTLFSLKLPDIAGNPFSEIDLFTGDEGDTYRNGRILEDGRIESRDPFYREDFTVL
                     YRWPHALLGNAPAPQTTNIFSHLLLPSTSSLLIWFPCLFLACGWLYLWKRRPQFTPVD
                     VIETDVIPPDYTPGMLRLDAKLVYDDKGFCADIVNLIVKGKIHLEAHYDKNQQILIRV
                     NEGATRNNAVLLPAEQLLLEALFRKGDKVVLTGRRNRVLRKAFLRMQKFYLPRKKSSF
                     YRPDAFLQWGGMAILAVILYGNLSPVGWAGMSLVGDMFIMICWLLPFLFCSLELLFAR
                     DDDKPCVNRVIITLFLPLICSGVAFYSLYINVGDVFFYWYMPAGYFSAVFLTGYLTGM
                     GYIFLPKFTQTGQQRYAHGEAIVNYLACKEAATHSGRRRKGEARKLDYALLGWAVSAN
                     LGREWAARITPSLTAAVRAPEIARSGVLFSLQMHLSLGANTSLLGRSYSGGGAGGGA"
BASE COUNT         2563 a         2817 c         2895 g         2721 t
ORIGIN      
        1 aattaccata tgtaatgtga tctgtgtagg atcatttgtt accccaatgt gggtatattc
       61 gtcacgtttt tataaccata acgacggagc ggatatgaaa aagttaacag tggcggcttt
      121 ggcagtagca actcttctct ctggcagtgc ctttgcgcat gaagccggcg aattttttat
      181 gcgtgcaggt tctgcaaccg tacgtccaac agaaggtgct ggtggtacgt taggaagtct
      241 gggtggattc agcgtgacca ataacactca actgggcctg acgtttactt atatggcgac
      301 cgacaacatt ggtgtggaat tactggcggc atcgccgttt cgtcataaag ttggcacagc
      361 cactaccggg gatattgcga ctgtccacca tttaccgcca acactgatgg cacaatggta
      421 ttttggcgat tcaggcagta aattccgtcc ttacattggt gcgggtatta actacaccac
      481 cttttttaat aatgacttca atgacaccgg tgaaggtctt ggtttatctg accttagtct
      541 gaaggattca tggggcgtgg cggggcaagt aggtctggat tatctcatca accgtgactg
      601 gttgctgaat atgtcggtct ggtacatgga tatcgatacc gaagtgaaat ataaatccaa
      661 cggcgcagtg ctgcctgcag ggcgttattc tgacaacgta cgccttgacc cgtgggtatt
      721 tatgttctct gcgggttacc gcttctaatt tttaaccatc aataaaccgc cagatgtttt
      781 ctggcggttt gtctcaaatt aacgtcgtgt agctgttttc atcggttgta caaaagcttt
      841 cagtgccgcc agcattttct ctggctcatt aatatgttgc tcgatgattt taacgatggc
      901 cgaaccagaa atcgcgcccg cagttcctgc atcaatcgct gcttttacct gatccggggc
      961 ggaaatacca aatccctgca atggaggtgc agcgttgtac tctttcagct tcgcaaccag
     1021 atgattgaga ggtaacgcgg cgcggttttc tgcgccggtc acgcccgcac gtgacaataa
     1081 ataggtgtat ccacgaccgt aggaggctat ctggcgcagc aaatcatcgt cggcattcgg
     1141 cgggcaaata aagataggtg cgacattatg acgcaacgcg gcctggcgga agggcgcgga
     1201 ctcttcaact ggcacatcgg caaccagcac cgaatcgacg ccgactttct cgcactcggc
     1261 ataaaactca tcaatgcctt tgttaaacac caggttggca tacatcaaaa ggccgatggg
     1321 aatggtcggg tgcttctggc gaatgagtgc cagcatctca aagcactgcg ccggggttac
     1381 tcccgccgca aaagcacgca gtgtggcgtt ttgaatcgtc gggccatccg ccagtgggtc
     1441 ggagaagggg atgcctaact ccagcgcgtc agcaccggct tcaattagcg tatcgataat
     1501 tttcaacgac tgctcaatgc ccggatcacc gagggtgacg aaaggaacga atgcgccttc
     1561 tttgcgctcc ttcaactggg caaacagaga ttcgtagcgt tccatcagat ttcccctcgt
     1621 gctttcaaaa tatcgtgaac ggtgaagatg tctttatcgc cgcgaccgga aaggttaacc
     1681 accagtagct gctctttttc cggattttcg cgcatcattt tcagcgcatg ggccagggcg
     1741 tgggaggatt ccagcgccgg gatgatccct tcatgcaggc aaagcgtttt aaaggcttcc
     1801 agggcttcat cgtcggtaat agacacgtaa tcagcgcgtc cagtgctgtt gagatacgca
     1861 tgttgcgggc cgacggacgg gaaatccagc ccggcagaaa tggagtaaga ctcttcaatt
     1921 tgcccgtctt cggtttgcat catcggcgct ttcataccga aatagatgcc cacgcgacca
     1981 tgttttaacg gtgcgccgtg ctcgccagtt tcgataccgt ggccgccagg ctccacacca
     2041 atcaggccga cgtcggtttc gttgatgaaa tctgcaaaca taccgatggc attcgaacca
     2101 ccgccaacac aggcgataac ggcatccggc aggcgacctt ctctttccag aatctgcgct
     2161 ttcgtttctt cgccaatcat ccgctgaaac tcacgcacaa tggtcgggta aggatgcggg
     2221 ccagctgcgg tacccagcat atagtgcgcg gtttcataac tgccggacca gtcgcgtagc
     2281 gcctcattac aggcatcttt cagggtcgcg gaaccgctat gtaccgggat cacttccgca
     2341 cccattaagc gcatccggaa aacgttaggc gactggcgtt caacgtcttt ggcacccata
     2401 taaatacggc atttcaggcc gagcagggcg ctggcaaggg ccgacgccac gccatgctga
     2461 ccggcaccgg tttcggcaat aatttcagtt ttacccatcc gcttcgccag taaagcctga
     2521 ccgagcacct ggttagtttt atgcgcgccg ccgtgcagca aatcttcgcg cttcagatac
     2581 agcgtggtgt tcgtcccggc tgtaatgttc tggcatttgg tcagcgcggt tggacgcccg
     2641 gcatagtttt tcagcaggtc gttgaactga gcctgaaatt caggatcttt ttgcgcgctg
     2701 acaaaagctt cttccagctg gcgcagagca ggcatcagga tttgtggcac gtacatgccg
     2761 ccaaactcac caaaataggg gttaagtaat gttgtcattg ttcctttcct taatatgcgc
     2821 gcagcgtctg gaaaaccgag gctaaaagac gtgcgtcttt gatacccggt tgcgactcta
     2881 cagcagaatt aaaatcaagc ccggcgcagc cggtttgtgc cgcttccacg cagttatctg
     2941 cgcctaagcc ccccgccagc agaacgttgc caagcgattg accatttaat agtgaccagt
     3001 cgaaacgttg tccgctcccg ccctgaccgt tgtcgaatac atatttatcg atgtgctgaa
     3061 aatcgcgcgc gggaagagtt tcaccgacac ttaaagcctt ccagatggcg acgtgtgctg
     3121 gcagagcctc acgcagagtg tcgatataca gctgatcttc attaccatgc agttgcactg
     3181 ccgccagcga taacacctta gctttgtcca ccacatcggc aatatcgtga ttgcggaaca
     3241 cgccaacata ctgcaacggt gccgcagcca tcacttcctg ggcctgttca acgttgacgc
     3301 aacgcggtga tgtcgcaaca aaaatcaacc caccgtaaat cgcgcccgcg tcataagctg
     3361 ctttagcatc ttgcccacgt gtcaggccac atactttatt ctcacccagc aacacccgac
     3421 gcacggcggc gttcaaatcg tcatgggcca tcaacgccga accaatcaga aagccgttag
     3481 cgaagtggct taactcgcgc acctgagcgt aagtattgat gccggattcg ctgattaccg
     3541 tcacgttgtg ccccagtttc ggtgcaagct cgcgggtacg gttgagatca atcgacaaat
     3601 cgcgcagatc gcggttgttg atgccaacga cctttgcccc caatgcaatg gcgcgctcca
     3661 gttcctcttc attactgact tcggtcagca cacccatctc cagactgtgg gcgacggctg
     3721 caagctggcg atattgttcg tcatccagta ctgaaagcat taataagcag gcatcggcct
     3781 ggtaatagcg cgccagatag atctggtaag gatcgataat gaagtcttta cataaaatcg
     3841 gctgcggggc gatttggctg acgatgggga ggaaatcaaa gctcccctga aaatatttct
     3901 catcagtcag cactgaaatt gccgaagcgt aatgtttata aatggcagca atgcgtgccg
     3961 gatcgaaatc atcacggatc acgccttttg acggcgacgc ttttttgcac tccagaataa
     4021 acgccgtgcg tgcaccctgt agcgcatcat aaaaatgtcg cgtgctcggc tgaatctcat
     4081 tctgaaaact ggccagcggt tgctgctgtt tgcgggcttc tacccaaatc gccttgtctg
     4141 cgacgatttt cgctaaaacg gtttgcatta tttaccctcg tgccgccagt gcggtgactc
     4201 tgtcgtaagc ggaaccactg cgcagtacct caagaacggt ttgcgcattg gcttgcagat
     4261 cttcatggcc atgcaggcgc attaacatgg cgacattcgc cgcgacggct gcttcgtgag
     4321 cggcgtcgcc tttaccttgt aacaagcgtg ttaaaatgtc acggttttct tccggtgttc
     4381 cgcctgccag ttgctcctgg tggtagggtg tcaggccaaa atcttcagcg gtcaattgat
     4441 agctcttaat ttcgccgtca tgcagttcgg caacgattgt cggcgcgtgt aatgaaactt
     4501 catccatccc gccgctgtgc accactgccg cgcgttgata ccccagcacg cgcaaggttt
     4561 cggcaatcgg cagcaccagc tccgggctgt agacaccaat taacgccagc ggaggatgcg
     4621 ccgggttaat taatggcccc agcacattga acagtgtgcg ggttttcagt tgctggcgaa
     4681 ccggcattgc atggcggaat ccggtgtgat atttcggtgc aaagaggaaa cagacgccta
     4741 attcatccag cgcctggcgc gatttatcgg cgttcatatc aagattaata ccgaacgccg
     4801 ccagcagatc cgacgagccg gatttactgg agacgctacg gttgccgtgt ttcgccactt
     4861 tcagcccgca ggccgcggcg acaaacgcac tggcggtaga aatattgatg ctgttgctgc
     4921 cgtcaccgcc agtaccgacg atatcggcaa acagataatc cgggcgcggg aacggcgcgg
     4981 cgttttccag tagcgcggtt gctgccccgg cgatctcgtt cgggtgctca ccgcgaattt
     5041 tcatgctcac cagcgccgcc gccagttgtt ccggcttcag ctcgccacgt accaccgctg
     5101 aaaacagctg gtggctttct tgttggctaa gcgtctgtgc ctgatacagt ttttccagaa
     5161 tcggttgcag cgtgttggtt tgctctagtt tctgctgcgc ccaggccagc gtttgttcca
     5221 gcaggcgagc gccctgggta gtaagaatgg attccggatg gaactggaat ccacaaacgc
     5281 gatccgcatc gtggcgcacc gccatcacca tgccattaaa atgggcgttg atggttaaac
     5341 cggccggaat attactgcca accagcgagt gataacgcgc cactggcagc gggtttgtta
     5401 atccggcaaa catcgcctga ccgtcatgtt caatgctcga cgctttaccg tgaagaattt
     5461 cgcccgcctg accgacatag cccccgtaag cttcgacaat cgcctgatgt ccgaggcaaa
     5521 tgccaataat tggcagcttg ccacgcaagc gggtgaggag ttccggcata caaccggctt
     5581 cgctcggcac accggggcca ggagaaagca tcagcaccgg attgctcatc gtcgccaggc
     5641 gttcaattaa ggtctgcgcc ggaatatggt tgcggtaaat caccacgtta tgaccattgc
     5701 tgcgcaactg atctgccagg ttgtaagtaa aagagtcgat attatcgagc agcagaatgt
     5761 cagccattag aacgtctcct gtgcatgatg cgcggtggca atagcgcgca gtacagcgcg
     5821 ggctttatta cgggtttcgt cggcttccga ctgcggaaca gaatcaagta ccacgccagc
     5881 accggcttgc acggtggcga ctccgttctc taccagcgcc gagcggatca caatgcaggt
     5941 gtcgagatcg ccatgcgcgg taaaataacc taccgcgccg ccgtagctgc cgcggcgacg
     6001 accttctgct tcggcaatta actgcatagc gcgtactttc ggcgcaccgc ttaacgtccc
     6061 catattcata caggcgcggt aagcgtgcag ggcgtcgaga tcgtggcgca gctcaccaac
     6121 aacgcgggag accaggtgca tcacgtaaga gtaacggtca actttggtga gatcggcgac
     6181 gtagcggctg ccgggggtgc aaatacgcgc caggtcatta cgggcgagat ccaccagcat
     6241 cagatgttca gaaagctctt tatgatcggt acgcatctcc agttcgatgc ggctgtcgag
     6301 gtctctatcc agtgaaccat cggcacgacg accgcgtgga cgtgttccgg caatcgggta
     6361 aatctcaatc tggcggctgg tggcgtcata cttgagcgaa ctttccggcg acgcgccaaa
     6421 cagggtgaaa tcattatcct gcataaaaaa catgtacggg ctgggattac tctttttcag
     6481 cacgtaatag gccgccagcg gtgacgggca gggcagagag aaacggcgag atggcaccac
     6541 ctggaaaatt tctccggcgc gaatcgcttt ttgcaacaaa cgcactacgc caccgaactc
     6601 ttcatcgctc tggttacatt cacaacgcat atgcggcacg gaaaccaccg gcagcggcgg
     6661 cgcggcttcg gtcagttgct gacgtagttc gttaaggcga gcagtgagac gttgtttttc
     6721 ttcttcattc ggagcaaaca ggctggcctg aatacgggtg ctttttttct gatggtcaat
     6781 caccatcagc gtttcagcga gataaaaaca gaaatcaggg cagttatttt ccgctgacag
     6841 ttgcggtaaa tcttcaaatc ccgccacaag gtcataagag aacaggccgc cgaagaacat
     6901 tgcttctcgt tcttccttcg gtacattcaa cagattctgt aataagcgga atgcgtcaaa
     6961 aaccgaaagg gagcataagc gggcgtcttc atccagcagt ggactgacag gcgggaagcg
     7021 cagtacgcgg cagtttggtg attgttcatt ttccacaccc gcaggcaggg cgttatccag
     7081 tagtgccagc agggcttcgc cattgccgga aagtgcctgg attgtgacgg tgtcacctaa
     7141 tgctgtaatg cgcagcgcac tgtctaccag tagcaggctt tttaaatcat ctttgctgtc
     7201 gatatctgcg gattccagca gcagcgttgc cggacgatcc ccacacaact ggtgaaaaag
     7261 cgcggttggg ttgtcgcgat aagcgcctac gcaggttagc agttcgagag tcggtttttg
     7321 tgtttgcatt gttattctct aattttgttc aaaaaaaagc ccgctcatta ggcgggctgg
     7381 gtatctgatt gctttacgca tggtgaatac actgcccgtt tcaggaagtg cgccaccaac
     7441 ctttcagtac gaaaattgct ttcattgtcg ataccctttt tacgtgaact tgcgtactag
     7501 ttaactagtt cgatgattaa ttgtcaacag ctcatttcag aatatttgcc agaaccgtta
     7561 tgatgtcggc gcaaaaaaca ttatccagaa cgggagtgcg ccttgagcga cacgaattac
     7621 tcagtgattt acgacctgca cagccatacc acagcttccg atggctgcct gacgccagaa
     7681 gcattggtgc accgtgcagt cgagatgcgc gtcggcaccc tggcgatcac cgaccatgac
     7741 accacagctg caattgcacc agcaagagag gaaatttcac gttcgggact ggcgctgaat
     7801 cttattcctg gtgtggaaat ttccacggtc tgggaaaatc atgaaattca tattgtcggg
     7861 ctgaatattg atattactca tccgctaatg tgtgagttcc tcgcgcagca gacagaacgg
     7921 cgcaatcagc gggcgcagct gattgccgaa agacttgaaa aagcacaaat acctggggca
     7981 ctggaaggcg cgcaacaact ggcgcagggc ggtgcggtga cgcgcggtca ttttgcgcgt
     8041 ttccttgtgg agtgcggaaa agccagttca atggcggatg tctttaaaaa gtatctggcg
     8101 cgcgggaaaa ccggatacgt tccgccacag tggtgtacaa tagaacaagc tattgatgtc
     8161 attcatcatt ctggcggtaa ggcggtgcta gctcatccag ggcggtacaa tcttagtgct
     8221 aaatggctga aaagattggt tgcgcatttt gccgaacacc acggcgacgc gatggaagtc
     8281 gcgcagtgcc agcaatcgcc caatgaacgc acccagctgg cgacacttgc gcgtcagcat
     8341 catttatggg catcacaagg atctgatttt catcaaccat gcccgtggat cgaactgggt
     8401 cgtaaactct ggctgcccgc aggcgttgaa ggcgtctggc agctatggga acagccgcag
     8461 aacaccacag agagggaatt atgagccagt ttttttatat tcatcctgat aacccgcagc
     8521 aacgcctgat caaccaggcg gtggagatcg tgcgtaaagg cggggtgatt gtttatccaa
     8581 ctgattccgg ctatgcgctc ggctgtaaaa ttgaagataa aaacgcgatg gagcgtattt
     8641 gtcgtattcg ccagctgccg gacggtcaca actttaccct gatgtgtcgc gatctttctg
     8701 aactctcaac ctattcattt gtcgataacg ttgcgtttcg tttgatgaaa aacaacacgc
     8761 caggcaacta taccttcatc ctgaagggga cgaaggaggt gccacgccgt ctgttgcagg
     8821 aaaaacgcaa aaccatcggt atgcgcgtgc cgtctaaccc gattgcgcag gcgctgcttg
     8881 aagcattggg cgaaccgatg ctttccactt cgctaatgct gccaggcagt gaatttaccg
     8941 aatccgatcc ggaagaaatt aaagaccgtc tggaaaaaca ggtggatttg attattcacg
     9001 gcggctatct cgggcagaaa ccaacaacgg ttattgatct caccgatgat acgccggtcg
     9061 tggtgcgtga aggtgtaggt gatgtgaagc ctttcttata acaaattgcg ttccacggat
     9121 ggaagatgat ggcagggata tatcgctgca ttttgctgtt gatagttggg ctttttttct
     9181 cttcgttgag ttatgcgaaa aacacggaga tcccttctta tgaagaaggg atctcgctct
     9241 ttgatgttga agccactctg caaccgaatg gggtgctcga catcaaagag aacattcatt
     9301 ttcaggcgcg aaatcagcag attaagcacg gattttatcg tgatttaccg cgactctgga
     9361 tgcagcctga tggggacgct gcactgctga actatcatat tgttggcgtc acccgtgatg
     9421 gtattcctga accctggcat cttgactggc atatcgggtt aatgagtatt gtcgtgggcg
     9481 ataaacaacg tttcttgcct caaggcgact atcattatca aattcattat caggttaaaa
     9541 atgctttcct gcgtgagggg gattctgctc tgctaatctg gaacgtgacc ggtaaccact
     9601 ggccgtttga aatatataag accctatttt cactcaagtt gcctgatatt gcgggtaatc
     9661 catttagcga aatcgatctc tttaccggag atgagggcga cacatatcga aatggccgca
     9721 tccttgagga cggaagaatt gaatctcgcg atccgtttta tcgtgaagat ttcacggtcc
     9781 tctaccgctg gcctcacgct ttacttggta atgccccggc accacaaacg acgaatattt
     9841 tcagccatct tcttttaccc tctacgtcat cgttgttaat ttggtttccg tgcctgttcc
     9901 tggcgtgtgg atggttatat ctctggaagc gcaggccgca atttacgccg gtagatgtga
     9961 ttgaaaccga tgtcattccg ccagattaca cgcccggcat gttacgtctc gatgcgaagc
    10021 tggtttatga cgataaaggt ttttgcgccg atatcgtaaa tctgattgtg aaaggaaaaa
    10081 ttcatctgga agctcattat gacaagaacc agcaaatcct gattcgtgtt aatgaaggcg
    10141 cgaccagaaa taatgcggta ttactgcccg cagagcagtt attactggaa gcgttatttc
    10201 gtaaaggcga taaggtcgtt cttacgggga gacgcaacag agtcttacgc aaggcatttt
    10261 tacggatgca gaaattttat ctgccgcgta aaaagtcttc gttttaccga cctgatgcgt
    10321 ttttgcaatg gggcgggatg gcgatattgg cggtcattct ctacggtaac ctgagtcccg
    10381 taggttgggc aggaatgagt ctggtaggcg atatgtttat tatgatctgc tggcttcttc
    10441 ctttcttatt ttgttccctt gagcttttgt ttgcccgcga tgatgacaag ccttgcgtta
    10501 atcgtgtaat catcactttg tttttaccac tgatttgttc aggcgtggcc ttctattctc
    10561 tctacatcaa tgtcggagat gtattctttt actggtatat gccagcgggt tattttagcg
    10621 ctgttttcct gaccggttat ctcactggca tggggtatat ttttctgcca aagtttaccc
    10681 aaactgggca gcaacgttat gcccacggtg aagctatcgt taactatctt gcgtgtaaag
    10741 aggcagcaac acacagtggg cggcggagga aaggggaagc acggaaactg gattacgctt
    10801 tgttaggttg ggcggtctcg gcaaaccttg gcagagaatg ggcagcacgt atcaccccat
    10861 cactcacagc ggctgtccgc gccccggaaa ttgcccgtag tggcgttttg ttctcattac
    10921 agatgcatct gagtctgggg gccaatacca gtttgttggg gcgaagttat tccggtggtg
    10981 gtgctggcgg cggtgc
//
LOCUS       DAHHTG010000135        10983 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.136, whole genome shotgun sequence.
ACCESSION   DAHHTG010000135 DAHHTG010000000
VERSION     DAHHTG010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10983)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10983)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10983
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.136"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            15..422
                     /locus_tag="NBI74_003737"
     CDS             15..422
                     /locus_tag="NBI74_003737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013317938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HCD1934013.1"
                     /translation="MDGHAKGTEMSVRDLVSYLLGWNALVVKWIASDAKGLPVDFPET
                     GYKWNQLGLLAQKFYSDYSELSYELLVAELQTVKNEIVNLINDRTDDILYGRPWYTKW
                     TMGRMISFNTSSPYANANGRLRKWAKNNNISLK"
     gene            441..1661
                     /locus_tag="NBI74_003738"
     CDS             441..1661
                     /locus_tag="NBI74_003738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEc53 family transposase"
                     /protein_id="HCD1934014.1"
                     /translation="MDEKSLYAHILNLTAPWQVKSLTLDENAGSVTVTVGIAENTQLT
                     CPTCRKSCSVHDHRHRKWRHLDTCQFMTLVEADVPRVMCPEHGCQTLPVPWAGSGSRY
                     TLLFESFVLSWLKISTVDAVRKQLKLSWNAVDGIMTRAVKRGLSRIKKPLSVRHMNVD
                     EVAFKKGHRYITVVSDRDGRALALTDDRGTESLASYLRSLTDSQLLAIKTLSMDMNAG
                     YIRAARIHLPNAVEKIAFDRFHVAKQLGEVVDKTRQNEHPHLPVESRRQAKGTRFLWQ
                     YSDKWMTESRQEKLMWLREQMQQTSQCWTLKELAKNIWDRPWSTERRNDWLQWISRAS
                     ECDVPMMKNAAKTIKKRLYGILNAMRHRVSNGNAEALNSKIRLLRIKARGYRNRERFK
                     LGVMFHYGKLNMAY"
     gene            2080..2982
                     /locus_tag="NBI74_003739"
     CDS             2080..2982
                     /locus_tag="NBI74_003739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1281 domain-containing protein"
                     /protein_id="HCD1934015.1"
                     /translation="MYFSGEPAKIAEIKRLASGAVTPFYRRATNEGIQLFLAGSAGLL
                     QTTEDVRFEPCPGLTAAGRGVLSPENITFTRWLKHLQDGVLLDEQNCLMLHELWLQSG
                     TGQRRWEGLPDDVRETITVHFTAKRGDWCDIWGNEDVSVWWNRLCDNVLSEKTMPFDL
                     LTVLPTRLDIEVNGFNGGVLNGVPSAYHWYTERYGVKWPCGYDLNISSQGENFIQVDF
                     DTPWCQPESDVVAELSRRFGCTLEHWYAEQGCNFCGWQLYERGELVDVLWGELEWSSP
                     TDDDELPEVTGPAWIVDNVAHYGG"
     gene            2979..3290
                     /locus_tag="NBI74_003740"
     CDS             2979..3290
                     /locus_tag="NBI74_003740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1934016.1"
                     /translation="MKYDGDGRATARFFSGKGCRRAPLFTAPADAARHKRCLWSVSRV
                     RWARDGRFYRSRLASVTVYASLSPFSDERPSSRFRGIALPSERRRLRYSTVGLTRYRT
                     R"
     gene            3366..4049
                     /locus_tag="NBI74_003741"
     CDS             3366..4049
                     /locus_tag="NBI74_003741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methylase"
                     /protein_id="HCD1934017.1"
                     /translation="MSRFVLGNCIDVMARIPDNAIDFILTDPPYLVGFRDRSGRTIAG
                     DVNDDWLQPASNEMYRVLKKDALMVSFYGWNRVDRFMAAWKRAGFSVAGHLVFTKNYT
                     SKSAYVAYRHECAYILAKGSPARPRNPLPDVLGWKYSGNRHHPTEKPVTSLQPLIESF
                     THPNAIVLDPFAGSGSTCVAALQSGRRYIGIELLEQYHRAGQQRLAAVQRAMQQGAAN
                     DNWFEPEAA"
     gene            4050..4271
                     /locus_tag="NBI74_003742"
     CDS             4050..4271
                     /locus_tag="NBI74_003742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1934018.1"
                     /translation="MNYAGHEKLRADVAEVANTMCDLRARLNDMEHRCRFDSDVLVER
                     LTRQTLYRANRLFMEAYTEILELDSCFKD"
     gene            4285..4719
                     /locus_tag="NBI74_003743"
     CDS             4285..4719
                     /locus_tag="NBI74_003743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 domain-containing protein"
                     /protein_id="HCD1934019.1"
                     /translation="MYGTCETLCRKLAAKYSGDTPLMLVIWSPEEIQALSDGMDIALT
                     DHEIRTVLARLEDIPEDQRTESGISSGVAMEIINNVSENRQVTVPAELLASLIQTAEQ
                     ALWKREWAAWDHGLAVPECVTRRQAVVNQVRILLKNNTHEND"
     gene            4764..5534
                     /locus_tag="NBI74_003744"
     CDS             4764..5534
                     /locus_tag="NBI74_003744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1934020.1"
                     /translation="MNEMLNALIYRHASNLLLAQGWPEETDVDQRNPKYPGWISIYVR
                     LDAPRLATLLIKRHGGVLPPLLASAIQKLTGTGAELVLSGSQWQSLPVLPADGTQVSF
                     PYAGEWLAEDEIRAVLGAVRDAVCSVSYQVAEDARRIRAALTTTGQTLLTRQTRRFRL
                     VVKESDHPCWLDEDDENLPVVLDAILNRGARFSSVEMYLVSECVEHILSSGLACDMLR
                     IPDEPPRRWFDRGVLREVVREARNEIRSMADALAKIRK"
     gene            6073..6375
                     /locus_tag="NBI74_003745"
     CDS             6073..6375
                     /locus_tag="NBI74_003745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HCD1934021.1"
                     /translation="MMPEYEGGFWHFIRLPDGGGYMMPDGDRFHLVNGENWFDRTVSA
                     DAAGIILTSLVINRQLWLYHDSGDAGLTHLYRMRDAQLWSHIEFHPECNAIYAALD"
     gene            6422..6844
                     /locus_tag="NBI74_003746"
     CDS             6422..6844
                     /locus_tag="NBI74_003746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 family protein"
                     /protein_id="HCD1934022.1"
                     /translation="MYCTVKEIIRDVLDTDVPDSECVFAVVLTRGDVRHIAQDWSLTD
                     DELETVMQRLDDAFVYGACDRVVSDIVNELMEEKRASRHVTVPAVMLEKVMALAGSEM
                     KRLYAVGSENGGDGDAFVREEREAMDVVLQALDGEHMS"
     gene            6841..7032
                     /locus_tag="NBI74_003747"
     CDS             6841..7032
                     /locus_tag="NBI74_003747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1934023.1"
                     /translation="MNISTETREILRNYRAVINARRREMGQKPLTTAQIVDEICDFVA
                     NQQAVFLGGHYILQGSRNR"
     gene            complement(7002..7482)
                     /locus_tag="NBI74_003748"
                     /pseudo
     CDS             complement(7002..7482)
                     /locus_tag="NBI74_003748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001358895.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            7995..8225
                     /locus_tag="NBI74_003749"
     CDS             7995..8225
                     /locus_tag="NBI74_003749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1934024.1"
                     /translation="MTISIVSPSAAAVKPRRHPRFRREDIPAPEIDPVLKAFGRHIAR
                     SFHRGRGVHIPAMKNTAFGQVLRTLELKRAFN"
     gene            8277..9638
                     /locus_tag="NBI74_003750"
     CDS             8277..9638
                     /locus_tag="NBI74_003750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3560 domain-containing protein"
                     /protein_id="HCD1934025.1"
                     /translation="MTLAAITMTAPEAASPVQMYRATYSPDDNKLRLYAASRLDPETY
                     KKVHDAGFRWAPKQALFVAPAWTPGREDVLLSLAGEIEDEDSTLAERQEARAERFTGY
                     SGKRASESAQALDEVERLAAMIPPGQPILVGHHSERRARRDAQRIENGMKRAVMLFER
                     AEYWEERARSALLHAKYKERPDVRWRRIKKIEADLRKAEKTIAQSQKYLTMWRAESLD
                     LNMAKLISSHDHISACFPLDTYPRPAEKSQYEGSRSLWSALDDDIITTEQAREIAIRC
                     HERQIQHQQRWVNHYQNRLIYERAMLDESGGVVTRTQDFEPGGQVFSRGEWLTIIRVN
                     KSNGAVSSVTTPNYSFLGYSGTMKVTPDRITDYKAPSAEEAAIASQAAKRPPVVNYPG
                     EGFREMTKAQWAALPRDCKAVRSVAEAEDHGAYRYRRTMDNNFRLVNVYITDMKITEI
                     PQK"
     gene            9685..10248
                     /locus_tag="NBI74_003751"
     CDS             9685..10248
                     /locus_tag="NBI74_003751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCD1934026.1"
                     /translation="MHSQLRERIRLMRARLDNAAPVAEIRAESQLFVTPAPVCDRLVT
                     LAEISNRDHILEPSAGTGAILRAIRDTAPEAMCDAVEINSGLVRYLRENFNGVRVQCG
                     DFMEWQPVQYYSRIIMNPPFSHGQDIRHILRAFSLLRPGGVLVAVCLNGPRQQEKLLP
                     FSDVREELPRGTFAYTDVPTMIIRLRA"
     gene            10400..10648
                     /locus_tag="NBI74_003752"
     CDS             10400..10648
                     /locus_tag="NBI74_003752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001380705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1934027.1"
                     /translation="MSVTDDVVVPATATATATALLFPAALILTTAVLSCLPGAPSAPG
                     CLAASGRQARLRQAAAGAPASLRRTRWRGFGLSLRSDA"
BASE COUNT         2435 a         2951 c         3213 g         2384 t
ORIGIN      
        1 cttcagataa atcaatggac ggacacgcca aaggaacgga gatgagtgtt cgtgatctcg
       61 tttcgtatct gcttggatgg aatgctcttg ttgtaaagtg gatcgcttct gatgctaaag
      121 gtctgcctgt cgattttccg gaaactggct ataaatggaa tcagcttggc cttcttgctc
      181 aaaaatttta ctcagattac agtgagttaa gttatgagtt gttagtagct gaacttcaga
      241 ctgtaaaaaa tgagattgtg aaccttatta atgatcgtac cgatgatatt ttgtatggaa
      301 gaccatggta cacaaaatgg acgatgggga gaatgatctc atttaacaca tcttcgcctt
      361 acgccaacgc taatggaaga ttaagaaagt gggcaaaaaa taataatatc agtttaaagt
      421 aagcttcaga atgaaattat atggacgaaa aatccctcta tgcccatatc cttaacctga
      481 ctgcaccatg gcaggttaaa tcccttaccc tcgatgaaaa tgcaggttcc gttactgtta
      541 cagtcggaat tgctgaaaat actcagttaa cctgtccgac ctgcaggaaa tcctgttctg
      601 ttcacgatca ccgacatcgt aaatggcgcc accttgatac ctgccagttc atgacattag
      661 tagaagccga tgttccccgc gttatgtgcc cggagcatgg ctgccagact ctgcctgtac
      721 cgtgggcagg ttccggcagt cggtacactc tgctgttcga atcgttcgtg ctctcatggc
      781 ttaaaatcag caccgttgat gcggtcagaa aacaacttaa acttagctgg aatgccgttg
      841 acggcatcat gacccgcgcg gttaagcgag gcctgtcgcg gattaaaaag cctttatcag
      901 tgcgtcatat gaatgtagac gaagtcgcct ttaaaaaagg gcataggtat ataaccgtgg
      961 tatctgatcg cgacgggcgg gcactggcat taaccgatga tcgtggcaca gagagtcttg
     1021 ccagctatct ccgttcgctt actgacagtc agttgttggc catcaaaaca ctgtcgatgg
     1081 acatgaatgc gggctatata agagcagcgc gtatccattt acccaatgcg gtcgagaaaa
     1141 tcgccttcga tcgcttccat gtggcgaagc aactgggcga agtggttgat aaaacccgcc
     1201 agaatgaaca tccgcacctc cctgttgaaa gtcgtcgtca ggccaaaggt acccgcttcc
     1261 tgtggcagta cagcgacaaa tggatgactg agtcccggca ggaaaagctg atgtggttgc
     1321 gggaacagat gcaacagaca agccagtgct ggacactgaa agagctggca aaaaatatct
     1381 gggatcgccc ctggagcaca gaacgcagga atgactggtt gcagtggata tcgcgggcgt
     1441 ctgaatgtga tgtgccgatg atgaaaaacg cagcgaaaac cattaaaaaa cggttatacg
     1501 gaatactgaa tgcaatgcgt catcgcgtct cgaatggaaa tgcggaggcg ctgaacagca
     1561 agatcagact gctgaggata aaggccaggg gataccgaaa ccgggaacgc tttaaactgg
     1621 gagttatgtt ccactatggg aaactgaata tggcgtactg agtcttccca ccatgatcgg
     1681 ggaagaccca tgtatataca gtattctaat aactgaatcc acacacagtc aaatgtcatt
     1741 attgtgacta aaaaacgctc aattctctga gggtgtggct gtatgcctgc tgtggactgc
     1801 tgtgtccagc ttatccacag cattttgtgc acggttctgt ggacaaaata cttggttacc
     1861 caggccgtgc cggcacgtta atcgggctgc atccgatgca agtgtgtcgc tgtcgccggc
     1921 ctcctcaccc ggtcacgttt cgtcgttcct cctccacgcg ctgcggcttc ggggccgcac
     1981 ctgcattcgt atgcggtcgc ccggttacag gtgcggcacg gcctgatgga ggccgtatgt
     2041 gagaggagaa ttcccatgcc aaaatggtgc tcgaatcgta tgtatttttc tggtgaaccg
     2101 gcaaagattg ctgagattaa acggctggcc agcggtgcag tcacaccgtt ttatcgccgc
     2161 gccacaaatg aaggtattca gctgtttctg gccggaagtg ccggacttct gcagaccact
     2221 gaagatgtgc ggtttgaacc atgccccgga ctgacggctg ccggacgtgg tgttttatcg
     2281 ccggagaata tcacgttcac ccgctggctg aagcacctgc aggacggtgt actgctggat
     2341 gagcaaaact gcctgatgct gcatgaactc tggctgcaga gcggtactgg acagcgtcgc
     2401 tgggaagggt taccggatga tgtcagggaa actatcactg tgcatttcac cgcaaaaaga
     2461 ggtgactggt gtgacatctg gggtaacgag gatgtatcgg tgtggtggaa ccgtctgtgt
     2521 gacaacgtac tgtcggaaaa aaccatgccg tttgacctgc tgacggtttt gccgacccgc
     2581 ctggatattg aagtgaatgg ctttaacggc ggtgttctga acggtgttcc ttctgcatat
     2641 cactggtata cagaacggta tggcgtgaag tggccttgtg ggtatgacct gaatatcagc
     2701 agtcaggggg aaaacttcat tcaggtagat ttcgacacgc cgtggtgtca gccggaaagc
     2761 gacgttgttg cagaattaag ccgccgtttc ggctgcacgc tggagcactg gtacgccgaa
     2821 cagggctgca atttctgtgg ctggcagttg tatgagcgcg gagagcttgt tgatgtgctg
     2881 tggggggaac tggaatggtc ttccccgaca gatgacgatg agctgccgga agtcaccgga
     2941 cctgcgtgga tagtcgacaa tgtggcgcat tatggcggat gaagtatgac ggagacgggc
     3001 gggcaaccgc ccgtttcttt tccggtaaag gatgtcgccg tgctcctctt tttactgcgc
     3061 cggctgacgc ggcgcggcat aaacgctgcc tgtggtcagt gtcccgcgtc cggtgggcac
     3121 gggacgggcg cttttaccgc tcccggctgg cgtcggtgac agtgtacgcc agcctgtcgc
     3181 ccttttctga tgaacgccct tcaagccgct ttcgcggcat agccttgccg tcagaaagac
     3241 gacggctgcg gtattccacg gtcggcctga cccgttaccg gacgcggtga acagcccaca
     3301 ggcagcgggg aacgggcacc acaggggtgc cctcccggtg ccctctgtaa gagaaggagt
     3361 tttgtatgtc ccgttttgtc ctcggtaact gcatcgatgt tatggcccgt attcctgata
     3421 acgccattga tttcatactg actgacccgc cgtacctcgt cggtttccgt gaccgttccg
     3481 ggcgcaccat tgccggtgat gttaatgatg actggctgca acctgccagc aatgagatgt
     3541 accgcgtact caaaaaagac gcgctgatgg tcagcttcta cggctggaac cgtgtcgatc
     3601 gctttatggc cgcctggaaa cgcgccggat tcagcgtggc agggcacctc gtgttcacca
     3661 aaaactacac atccaaatct gcgtatgtcg cgtaccgtca cgaatgcgct tacatcctgg
     3721 caaagggcag tccggcccgg ccccgtaacc ctctgccgga tgtgctgggc tggaaatatt
     3781 caggcaatcg tcatcacccg acggaaaagc ccgttaccag cctgcaacca ctgattgaga
     3841 gcttcacaca tccgaacgcg attgtgctgg acccgtttgc aggcagcggc tcaacctgcg
     3901 tcgctgccct ccagtccgga cgccggtata tcggtatcga gctgcttgaa cagtatcacc
     3961 gtgccggaca gcaacgactg gccgccgtgc aacgggccat gcagcagggg gccgcgaatg
     4021 ataactggtt tgaaccggag gctgcgtaaa tgaactatgc cggacacgaa aaacttcgcg
     4081 ccgacgtggc ggaggtggcc aacaccatgt gtgaccttcg ggccaggctg aacgatatgg
     4141 agcatcgctg tcgctttgat tccgatgtgc ttgttgaacg cctgacacgc cagacgctgt
     4201 atcgcgccaa tcgcctgttc atggaggcgt ataccgaaat tctggaactg gattcctgct
     4261 tcaaagattg agaaggagac gagaatgtac ggaacatgcg aaacgctatg ccggaagctg
     4321 gcagcaaagt attcgggaga cacgccactg atgctggtta tctggtcccc ggaagaaatt
     4381 caggccctct ctgacggaat ggatattgcc ctgaccgatc atgaaatcag gactgtcctg
     4441 gcgcgcctgg aggacatccc ggaagaccag cggactgaat ccggtatttc ttccggcgtg
     4501 gcgatggaga tcatcaataa cgtgagcgaa aaccgccagg tgactgtccc tgctgaactg
     4561 ctggcgtccc tgattcagac cgctgaacag gcattgtgga aacgtgaatg ggccgcctgg
     4621 gatcatggcc tcgccgtccc ggaatgcgtc acccgccgtc aggcggtggt taatcaggtc
     4681 cgcatcctgc tgaaaaacaa cacacacgaa aacgactgat gttatcgccg cctccgggcg
     4741 gcgatacagg gagttcatga attatgaatg aaatgctgaa tgcactgatt tatcgccacg
     4801 ccagcaacct gttgctggcg cagggctggc cggaagagac ggatgttgac cagcgtaacc
     4861 cgaagtatcc gggctggatc agcatttacg tgcggctgga tgcgccccgg ctggcgacgt
     4921 tacttatcaa gcgtcacggc ggcgtactgc cgccgctcct ggcctctgcc attcagaaac
     4981 tgaccggaac cggagcggaa cttgttctgt ccggcagtca gtggcagtcg ctgccggtac
     5041 ttccggcaga cggaacgcag gtgtctttcc cgtatgccgg tgagtggctg gcagaggacg
     5101 aaatcagggc cgttcttggt gcggtacgcg atgcagtatg cagtgtcagt taccaggtgg
     5161 cagaagatgc gcggcgtatc cgtgcggcgc tgaccaccac cggtcagacg ttgctgaccc
     5221 gccagacgcg ccgctttcgc ctggtggtga aggaaagcga tcacccctgc tggctcgatg
     5281 aagacgacga aaacctgccc gtggtgctcg atgccatcct gaaccggggc gctcgttttt
     5341 cgtcggtgga aatgtatctg gtcagtgagt gtgtggagca tatcctgtcc agtgggctgg
     5401 cctgcgatat gctgcgtata ccggatgaac cgccccgccg ctggtttgac cgtggtgttc
     5461 tgcgggaggt ggtccgggaa gcccggaacg aaatccgcag catggcggat gccctggcaa
     5521 aaatccggaa atgatgacgc tggcggggaa aaatctccgc cttttccagt cggtccccgg
     5581 aaagagcaaa aggatgtcgc cgcaggctcc tctttacccg gccatgtggg gcatggcctt
     5641 gtgggttttc agttatgtgg cctcagcgtc gtgtgcgggc tgtgccgtgc ctccatcgta
     5701 gccgggcttc tgcgggcggg ttgcaaggta aggtgaaccg ctgcggtcac ccggtccctc
     5761 cttcccgtct gccgtgattt tccggtcgtt cacccgctca gatttcgcgg tctcaccctc
     5821 caactcccgt cagccgtgcg cgtcaggctg cggcttccct tgcgcccttg caaccctgca
     5881 ggcccggcaa agatggaggc acggcaccgc ccggtgtcgc ggaatatgtg aacaacggag
     5941 gaaaacatca tgcaatatgc gaaacctgtc actctgaatg ttgaagagtg cgaccgtctc
     6001 tcctttctgc cttacctgtt tggcctggat tttctgtatg ccgaggcatc tgtgtacacg
     6061 ctggcgaaaa aaatgatgcc ggaatatgaa ggcggattct ggcacttcat ccgcctgccg
     6121 gacggtggcg gttacatgat gccggatggc gaccgttttc atctggttaa cggtgaaaac
     6181 tggtttgacc gtaccgtgag tgctgatgcc gcaggcatca tccttacctc ccttgtgatt
     6241 aaccgccagt tgtggctgta ccacgacagc ggggatgcag ggttgacaca cctgtacagg
     6301 atgcgcgatg cgcagttgtg gagccacatc gaatttcacc ccgaatgcaa cgcgatttac
     6361 gcggcacttg actgattaac ggacggggcg gcaatgccgc ccctgtaaag gagacgagaa
     6421 catgtactgt actgttaaag aaatcatccg cgatgtactg gatacagacg tgccggacag
     6481 tgaatgcgtt tttgccgtgg tgctgacccg tggggatgtg cgtcacatag cccaggactg
     6541 gagtctgaca gacgatgagc tggaaaccgt catgcagcgg ctggacgatg cttttgtata
     6601 tggcgcatgt gacagagttg tttccgatat tgtaaatgaa ctgatggaag aaaagcgcgc
     6661 cagccgtcat gtgacagtcc cggcggtgat gctggaaaaa gtgatggcgc tggcaggcag
     6721 tgaaatgaag cgcctgtatg ccgtcgggag tgagaacggg ggcgacggtg acgcattcgt
     6781 cagggaggaa cgcgaagcaa tggacgttgt gttacaggcg ctggacgggg agcacatgtc
     6841 atgaatatca gcacagaaac ccgcgaaatt ctgcgtaatt acagggccgt gattaatgcg
     6901 cggcgtcgtg aaatggggca gaaaccgctc accaccgcgc agattgttga tgaaatctgc
     6961 gattttgtgg cgaatcagca ggcggttttc ctgggcggtc actacatctt acagggcagc
     7021 agaaacaggt gatacagggg caggcggtgg aggccgcctg tcagcccgcg cgaacccccc
     7081 tggcaggctg gcgcctgccg gaagggtaac gggccacgct ggcgcgtcgc ccgttaccct
     7141 tcccgtttgt gatgtgtccg gaatgttttt tcctgatgtt atgtttctgt ggttactgtt
     7201 cttcttcttc ctcttctttc cgttcctctc ctgtttatgg ctttcccggg cgtcccccag
     7261 gcggggcggg ctgtcgtgaa cggagccagc ggagctgtct ccgccctggc gggcttccat
     7321 ccctgacgca aaacccccgg cttgcagcga ccgttccggt cgcgccgccc ctgtcgcgcc
     7381 gtggcctgcg ctcctggcat cccggcaaaa cgacgccggg atgtaaggcg caacgccgtt
     7441 tcgcgcacgc gctctccgtc gttgcggcgg gtccctgctc attgcctgcc gggtgccaca
     7501 cacccgtatg agtccctgtc gccgggggca tagacagcca caacctgccg cactgactgt
     7561 ccagggtaaa tgaacagccc acaaaatttt ttacccaaaa cccggtaaaa aattttgcgt
     7621 tctgcccagg acaggtgcgt caggccgtgg cagtgatgcc ccttgcggcg aaggttctca
     7681 aacgggtgaa gttcatacca cagggaatac agcagcaggt tcccgcgaaa cgtcaaaacc
     7741 gcggctccca aaactcaacg ggtgatgttc tttaaaaatc aggatttatt tcaggctccg
     7801 gcaatacgca tttatgtgtt ctgtcagtca tccggctggc aggtaatgag aacgcaggaa
     7861 cagaaggagg aaaacaccgg aaaacgtcag atgcagatat gaaaaaagca ggtcagggac
     7921 ctgcttaatc catgccgcag agactgcacg aacagttttt gcggctccag tcaatcgtca
     7981 taagaggcta aattatgaca ataagtattg tttcaccttc cgcagcggct gtaaagcccc
     8041 gccgtcatcc ccgtttcaga cgcgaagaca tccccgcgcc ggaaattgac cccgtgctga
     8101 aagccttcgg gcgacacata gcccggagtt ttcaccgggg gcgtggtgta cacatccccg
     8161 ccatgaaaaa cacggctttc gggcaggtgc tccgcacact cgaactgaaa cgcgctttta
     8221 actgatgagg tgagccgccc cgcgaggggc ggcaacagac ggaaggagaa cattttatga
     8281 cattagcagc aatcaccatg accgccccgg aagccgccag ccctgtgcag atgtaccgcg
     8341 cgacctactc accggatgac aacaaactgc gcctgtatgc cgcgtcacgt cttgacccgg
     8401 agacgtataa aaaagtgcat gatgccggtt tccgctgggc acccaaacag gcgctgtttg
     8461 tcgcgccagc ctggacaccg ggccgggaag acgtgctcct ctcacttgcc ggagagattg
     8521 aggatgaaga cagcacgctc gctgaacgtc aggaagcacg ggcggagcgg tttaccggat
     8581 acagcggaaa gcgggccagt gaatccgcac aggcacttga tgaagtggaa agactggccg
     8641 cgatgatccc gcccggtcag cccattcttg tggggcatca cagcgaacgc cgcgcccgtc
     8701 gtgatgcgca gcgtattgaa aacggcatga aacgtgccgt gatgctcttt gaacgtgcgg
     8761 aatactggga agaacgggcg cggtcggcac tgcttcacgc gaagtataaa gaacgcccgg
     8821 acgttcgctg gcgtcgtatc aaaaaaatcg aagctgattt gcgcaaggct gaaaagacca
     8881 tcgcacagtc gcagaaatat ctgacgatgt ggcgggctga atcgctggat ctgaatatgg
     8941 caaaactcat cagcagtcat gaccatatca gcgcctgttt cccgctggat acgtatccgc
     9001 gcccggcaga aaaaagccag tatgaaggga gccggtcgtt atggtcggcc ctggatgatg
     9061 acatcatcac cacggagcag gcccgcgaaa ttgcgatccg ctgtcatgaa cggcagattc
     9121 agcatcagca acgctgggtt aaccactatc agaaccgcct gatctatgag cgtgccatgc
     9181 tggacgaaag cggcggcgtg gttacccgga cacaggattt tgagccgggc ggacaggttt
     9241 tcagccgggg cgagtggctg accatcatcc gcgtgaacaa aagcaacggg gcggtgagtt
     9301 cagtcacaac gccgaattac agttttctcg ggtacagcgg cacgatgaaa gtgacgcccg
     9361 atcgcatcac ggactacaaa gcaccatcgg cagaagaggc tgccatcgcc agccaggccg
     9421 cgaagcgtcc gccggtagtc aactatccgg gggaaggttt ccgggaaatg acaaaggcac
     9481 agtgggccgc cctgccccgg gactgtaagg ccgtgcgcag tgtggcagaa gcagaagacc
     9541 acggggcata ccgctaccgc cgcacaatgg acaataattt ccgcctggtg aatgtgtata
     9601 tcaccgacat gaaaatcacg gaaatcccac agaaataagg tatatccccc cgggaaatcc
     9661 ccgggggatg acggagataa tgacatgcac agtcagttaa gagaacgtat ccggctgatg
     9721 cgcgcaaggc tggataacgc cgcgccggtt gctgaaatcc gggctgaatc tcagcttttt
     9781 gtgactcctg ccccggtctg tgatcgcctg gtgacgctgg cggaaatcag caaccgtgat
     9841 cacattctgg aaccctctgc cggcaccggg gccatactgc gggcgattcg ggataccgcg
     9901 ccggaggcca tgtgtgatgc ggtggagatt aacagcgggc tggtccggta tctgcgggaa
     9961 aattttaacg gtgtcagggt acagtgtggc gacttcatgg aatggcagcc ggtgcagtat
    10021 tacagccgga tcattatgaa tccgccgttc agtcacgggc aggatatccg gcatatcctg
    10081 cgggcttttt ccctgttgcg tccggggggc gtgctggttg ccgtctgtct caacgggccg
    10141 cgccagcagg agaagctgtt accgttttct gacgtccgcg aggagctgcc gcgcggcacg
    10201 tttgcttata ccgatgtccc gacgatgatt attcgtttgc gtgcctgatg acacagggct
    10261 ggcgggagga taaatgcccg tctgtccgca ccgggaaaag accgcgcgcc ggtggcgctg
    10321 aaaggggccg cgctcacgcg ccgccccttt cattacgcgg acgttctgat gggggcttct
    10381 gtcttttcag acctgtcatt tgtctgttac tgatgatgtt gttgttcctg ctactgctac
    10441 tgctactgct actgctcttc tttttcctgc cgctctgatt ctgactactg cagtgttgtc
    10501 ctgtctgccg ggcgcacctt cggctcccgg ctgcctggcg gcatccggtc gtcaggcccg
    10561 actccgacag gcagctgctg gcgcacccgc cagtctccgc cgaacccgct ggcgcggttt
    10621 cgggctgtcg ctacggtccg atgcgtgaaa accccgggct tgcagcgacc gttccggtcg
    10681 cgccgcctct gtcgcgccgt ggcctgcgct ccaggcatcc cggcaaaaag acgccgggat
    10741 gtaaggcgca acgccgtttc gcaaaccgcg cttcacgtcg ttgcggcggg tccctgcccg
    10801 ttgttgtgtc cggtgccatt cacctgttta agcccctgtc gccgggggct gatacagcca
    10861 caacctgccg cacctgtcca gggtaaatga acagcccaca aaatttttta cccaaaaccc
    10921 ggtaaaaaat tttgcgttct gcccaggaca ggtgcgtcag gccgtggcag tgatgcccca
    10981 tgc
//
LOCUS       DAHHTG010000136        10831 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.137, whole genome shotgun sequence.
ACCESSION   DAHHTG010000136 DAHHTG010000000
VERSION     DAHHTG010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10831)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10831)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10831
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.137"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            <1..266
                     /locus_tag="NBI74_003753"
     CDS             <1..266
                     /locus_tag="NBI74_003753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Lon protease family protein"
                     /protein_id="HCD1934028.1"
                     /translation="VIIPTANVRHLSLHSELVKAVEEGKFTIWAVDDVTDALPLLLNL
                     VWDGEGQTTLMQTIQERIAQASQQEGRHRFPWPLRWLNWFIPN"
     gene            335..853
                     /gene="fabA"
                     /locus_tag="NBI74_003754"
     CDS             335..853
                     /gene="fabA"
                     /locus_tag="NBI74_003754"
                     /EC_number="4.2.1.59"
                     /EC_number="5.3.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014915875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-hydroxydecanoyl-ACP
                     dehydratase/trans-2-decenoyl-ACP isomerase"
                     /protein_id="HCD1934029.1"
                     /translation="MVDKRESYTKEDLLASGRGELFGAKGPQLPAPNMLMMDRVVKMT
                     ETGGNFDKGYVEAELDINPDLWFFGCHFIGDPVMPGCLGLDAMWQLVGFYLGWLGGEG
                     KGRALGVGEVKFTGQVLPTAKKVTYRIHFKRIVNRRLIMGLADGEVLVDGRLIYTAND
                     LKVGLFQDTSAF"
     gene            complement(923..1090)
                     /gene="rmf"
                     /locus_tag="NBI74_003755"
     CDS             complement(923..1090)
                     /gene="rmf"
                     /locus_tag="NBI74_003755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome modulation factor"
                     /protein_id="HCD1934030.1"
                     /translation="MKRQKRDRLERAHQRGYQAGIAGRSKEMCPYQTLNQRSQWLGGW
                     REAMADRVVMA"
     gene            complement(1346..1909)
                     /gene="pqiC"
                     /locus_tag="NBI74_003756"
     CDS             complement(1346..1909)
                     /gene="pqiC"
                     /locus_tag="NBI74_003756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309063.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiC"
                     /protein_id="HCD1934031.1"
                     /translation="MKKWLVTIAALWLAGCSSGEINKNYYQLPVVQSGTQSTASQGNR
                     LLWVEQVAVPDYLAGNGVVYQTSDVKYVIANNNLWASPLDQQLRNTLVANLSTQLPGW
                     VVASQPLGSAQDTLNVTVTEFNGRYDGKVIVSGEWLLNHQGQLIKRPFRLEGVQTQDG
                     YDEMVKVLARVWSQEAASIAQEIKRLP"
     gene            complement(1906..3546)
                     /gene="pqiB"
                     /locus_tag="NBI74_003757"
     CDS             complement(1906..3546)
                     /gene="pqiB"
                     /locus_tag="NBI74_003757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intermembrane transport protein PqiB"
                     /protein_id="HCD1934032.1"
                     /translation="MESNNGEAKIQKVKNWSPVWIFPIVTALIGAWVLFYHYSHQGPE
                     VTLITANAEGIEGGKTTIKSRSVDVGVVESATLADDLTHVEIKARLNSGMEKLLHKDT
                     VFWVVKPQIGREGISGLGTLLSGVYIELQPGAKGSKMDKYDLLDSPPLAPPDAKGIRV
                     LLDSKKAGQLSPGDPVLFRGYRVGSVETSTFDTQKRNISYQLFINAPYDRLVTSNVRF
                     WKDSGIAVDLTSAGMRVEMGSLTTLLSGGVSFDVPEGLDLGQPVAPKTAFVLYDDQKS
                     IQDSLYTDHIDYLMFFKDSVRGLQPGAPVEFRGIRLGTVSKVPFFAPNMRQTFNDDYR
                     IPVLIRIEPERLKMQLGENADVVEHLGELLKRGLRGSLKTGNLVTGALYVDLDFYPNT
                     PAITGIREFNGYQIIPTVSGGLAQIQQRLMEALDKINKLPLNPMIEQATSTLSESQRT
                     MKNLQTTLDSMNKILASQSMQQLPTDMQSTLRELNRSMQGFQPGSAAYNKMVADMQRL
                     DQVLRELQPVLKTLNEKSNALVFEAKDKKDPEPKRAKQ"
     gene            complement(3551..4804)
                     /gene="pqiA"
                     /locus_tag="NBI74_003758"
     CDS             complement(3551..4804)
                     /gene="pqiA"
                     /locus_tag="NBI74_003758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiA"
                     /protein_id="HCD1934033.1"
                     /translation="MCEHHHAAKHILCSQCDMLVALPRLEHGQKAACPRCGTTLTVAW
                     DAPRQRPTAYALAALFMLLLSNLFPFVNMNVAGVTSEITLLEIPGVLFSEDYASLGTF
                     FLLFVQLVPAFCLITILLLVNRAELPVRLKEQLARVLFQLKTWGMAEIFLAGVLVSFV
                     KLMAYGSIGVGSSFLPWCLFCVLQLRAFQCVDRRWLWDDIAPMPELRQPLKPGVTGIR
                     QGLRSCSCCTAILPADEPVCPRCSTKGYVRRRNSLQWTLALLVTSIMLYLPANILPIM
                     VTDLLGSKMPSTILAGVILLWSEGSYPVAAVIFLASIMVPTLKMIAIAWLCWDAKGHG
                     KRDSERMHLIYEVVEFVGRWSMIDVFVIAVLSALVRMGGLMSIYPAMGALMFALVVIM
                     TMFSAMTFDPRLSWDRQPESEHEES"
     gene            complement(4934..6841)
                     /locus_tag="NBI74_003759"
     CDS             complement(4934..6841)
                     /locus_tag="NBI74_003759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCD1934034.1"
                     /translation="MSLISMHGAWLSFSDAPLLDNAELHIEDNERVCLVGRNGAGKST
                     LMKILNREQGLDDGRIIYEQDLIVARLQQDPPRNVEGSVYDFVAEGIEEQAEYLKRYH
                     DISRLVMNDPSEKNLNELAKVQEQLDHHNLWQLENRINEVLAQLGLDPNVALSSLSGG
                     WLRKAALGRALVSNPRVLLLDEPTNHLDIETIDWLEGFLKTFNGTIIFISHDRSFIRN
                     MATRIVDLDRGKLVTYPGNYDQYLLEKEEALRVEELQNAEFDRKLAQEEVWIRQGIKA
                     RRTRNEGRVRALKAMRRERGERREVMGTAKMQVEEASRSGKIVFEMEDVCYQVDGKQL
                     VKDFSAQVLRGDKIALIGPNGCGKTTLLKLMLGQLQADSGRIHVGTKLEVAYFDQHRA
                     ELDPDKTVMDNLAEGKQEVMVNGKPRHVLGYLQDFLFHPKRAMTPVRALSGGERNRLL
                     LARLFLKPSNLLILDEPTNDLDVETLELLEELIDSYQGTVLLVSHDRQFVDNTVTECW
                     IFEGGGKIGRYVGGYHDARGQQEQYVALKQPAVKKSEEAAAPKAETVKRSSSKLSYKL
                     QRELELLPQLLEDLEAKLEALQTQVADASFFSQPHEQTQKVLADMAAAEQELEQAFER
                     WEYLEALKNGG"
     gene            complement(6853..8961)
                     /gene="rlmKL"
                     /locus_tag="NBI74_003760"
     CDS             complement(6853..8961)
                     /gene="rlmKL"
                     /locus_tag="NBI74_003760"
                     /EC_number="2.1.1.173"
                     /EC_number="2.1.1.264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 23S rRNA
                     (guanine(2069)-N(7))-methyltransferase RlmK/23S rRNA
                     (guanine(2445)-N(2))-methyltransferase RlmL"
                     /protein_id="HCD1934035.1"
                     /translation="MNSLFASTARGLEELLKTELENLGAVECQVVQGGVHFKGDTRLV
                     YQSLMWSRLASRIMLPLGECKVYSDLDLYLGVQAINWTEMFNPGATFAVHFSGLNDTI
                     RNSQYGAMKVKDAIVDAFTRKNLPRPNVDRDAPDIRVNVWLHKETANIALDLSGDGLH
                     LRGYRDRAGIAPIKETLAAAIVMRSGWQPGTPLLDPMCGSGTLLIEAAMLATDRAPGL
                     HRGRWGFSGWAQHDEAIWQEVKAEAQTRARKGLAEYSSHFYGSDSDARVIQRARTNAR
                     LAGIGELITFEVKDVAQLTNPLPKGPYGTVLSNPPYGERLDSEPALIALHSLLGRIMK
                     NQFGGWNLSLFSASPDLLSCLQLRADKQYKAKNGPLDCVQKNYHVAESTPDSKPAMVA
                     EDYANRLRKNLKKFEKWARQEGIECYRLYDADLPEYNVAVDRYADWVVVQEYAPPKTI
                     DAHKARQRLFDIIAATISVLGIAPNKLVLKTRERQKGKNQYQKLGEKGEFLEVTEYNA
                     HLWVNLTDYLDTGLFLDHRIARRMLGQMSKGKDFLNLFSYTGSATVHAGLGGARSTTT
                     VDMSRTYLEWAERNLRLNGLTGRAHRLIQADCLAWLREANEQFDLIFIDPPTFSNSKR
                     MEDAFDVQRDHLALMKDLKRLLRAGGTIMFSNNKRGFRMDLDGLAKLGLKAQEITQKT
                     LSQDFARNRQIHNCWLITAA"
     gene            9059..10168
                     /gene="ycbX"
                     /locus_tag="NBI74_003761"
     CDS             9059..10168
                     /gene="ycbX"
                     /locus_tag="NBI74_003761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-N-hydroxylaminopurine resistance protein YcbX"
                     /protein_id="HCD1934036.1"
                     /translation="MATLTRLFIHPVKSMRGIGLTHTLADVSGLAFDRIFMITEPDGT
                     FITARQFPLMVRFTPSPVHDGLHLTAPDGSSAYVRFADFATQDAPTEVWGTHFTARIA
                     PDAINKWLSGFFSREVQLRWVGPQMTRRVKRHNTVPLSFADGYPYLLANEASLRALQQ
                     RCPASVKMEQFRPNLVVSGASAWEEDSWKVIRIGDVVFDVVKPCSRCIFTTVSPEKGQ
                     KHPADEPLKTLQSFRTAQDNGDVDFGQNLIARNSGVIRVGDEVEILATAPAKIYGAGA
                     ADDTANITQQPDANVDIDWQGQAFRGNNQQVLLEQLENQGIRIPYSCRAGICGSCRVQ
                     LLEGEVTPLKKSATGDDGTILCCSCVPKTALKLAR"
     gene            complement(10165..10707)
                     /gene="zapC"
                     /locus_tag="NBI74_003762"
     CDS             complement(10165..10707)
                     /gene="zapC"
                     /locus_tag="NBI74_003762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415466.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapC"
                     /protein_id="HCD1934037.1"
                     /translation="MRIKPDDNWRWYYDEEHDRMMLDLANGMLFRSRFARKMLTPDAF
                     SPAGFCVDDAALYFSFEEKCRDFNLSKEQKAELVLNALVAIRYLKPQMPKSWHFVSHG
                     EMWMPMPGDAAYVWLSDTHEQVNLLVVESGENAALCLLAQPCVVIAGRAMQLGDAIKI
                     MNDRLKPQVNVDSFSLEQAV"
BASE COUNT         2627 a         2938 c         2592 g         2674 t
ORIGIN      
        1 gtgtcattat ccccactgct aacgttcgcc atttaagtct tcacagtgaa ctggtgaaag
       61 cggtagaaga aggcaaattc accatctggg cagtagacga tgtgactgac gcactgccgt
      121 tattattaaa tctggtgtgg gatggcgaag gccagacgac gctgatgcaa accatccagg
      181 aacgtatcgc acaagcatcg caacaggaag gacgtcaccg ttttccatgg ccattacgtt
      241 ggctgaactg gtttattccg aactgatcgg acttgttcag cgtacacgtg ttagctatcc
      301 tgcgtgcttc aataaaataa ggcttacaga gaacatggta gataaacgcg aatcctatac
      361 aaaagaagac cttcttgcct ctggtcgcgg tgaactgttt ggcgctaaag gcccgcaatt
      421 gccagcacca aacatgctga tgatggaccg tgtggtcaaa atgaccgaaa cgggtggtaa
      481 cttcgacaaa gggtatgttg aagcagagct ggatatcaat ccggatctgt ggttcttcgg
      541 atgccacttc attggtgatc cggttatgcc gggatgcctg ggcctggacg caatgtggca
      601 gctggtaggg ttctacctcg gctggctggg cggcgaaggt aaaggtcgcg cgttgggcgt
      661 aggtgaagtg aaattcacag gtcaggtgct gccgacggcg aaaaaagtga cctatcgtat
      721 tcactttaaa cgcatagtta accgtcgtct gattatgggc ctggctgatg gcgaagtgct
      781 ggttgatggt cgtttgatct ataccgccaa cgacctgaaa gtcggtctgt tccaggatac
      841 gtctgccttc tgataataat cagacagagt ataaaaaggc gaaacctccg caatgcggag
      901 gtttcttttt aaagagacag aatcaggcca ttactaccct gtccgccatg gcttctcgcc
      961 agcctcccag ccattgtgac ctttgattca gcgtctgata gggacacatt tcttttgagc
     1021 gtccggcgat gccggcctga taaccacgtt gatgtgcccg ttccaggcga tctcgttttt
     1081 gtctcttcat gcctcgtttc cctcatactg gtttctggtg gaaaagaaaa cagtgattac
     1141 taaatatgca accacagtta agcaataccg cgaatcataa ctgccgtcaa tgcgcaaaat
     1201 tcacgccact gtcatatttg tgagctacac gggcatgtcg gttgtacaaa aaatgtgaag
     1261 aggaacaact ttttccgcac gttagaaaca aaaaaaccgc ctcaggcaac ataccggaga
     1321 cggttatatt tacaaatctt tatgattaag gtagacgctt tatctcttgc gcaatagaag
     1381 cggcttcctg actccagaca cgggccagca ctttaaccat ctcatcgtaa ccatcctgag
     1441 tttgcactcc ttccagacgg aacggacgtt tgatcagttg tccctggtgg ttcaacagcc
     1501 actcaccact gacaatgacc ttgccatcat agcgaccgtt aaactccgtg acggtaacat
     1561 tgagcgtgtc ctgggcgctt cccagaggct gggaggcaac cacccagccg ggcagttgcg
     1621 tactcaggtt ggcaaccagg gtgttgcgca actgttgatc caacgggctg gcccacaagt
     1681 tgttgttggc aatcacatac ttcacatcac tggtttgata aaccacacca ttccccgcca
     1741 gatagtcagg aacagcgacc tgctcaaccc ataacaggcg attaccctgg ctggcggtgc
     1801 tttgtgtacc gctctgcacc acaggtaact ggtaatagtt tttattaatt tcaccggagc
     1861 tgcatccggc cagccacagt gccgcaatcg tcactagcca ctttttcatt gtttcgccct
     1921 cttcggctct ggatcttttt tgtccttcgc ttcaaatacc agcgcgttac tcttctcgtt
     1981 gagcgttttc agcaccggtt gcagttctcg caacacctga tcaaggcgct gcatatccgc
     2041 caccatcttg ttgtaggctg cggaaccagg ctggaagccc tgcatactgc gattcaattc
     2101 acgcaacgtt gactgcatat ccgtcggtaa ctgctgcatc gactggctgg cgaggatctt
     2161 gttcatgcta tccagcgtcg tttgcaggtt tttcattgtg cgctgacttt cagaaagcgt
     2221 actggttgcc tgttcaatca tcggattcag tggcagtttg ttgatcttat ccaacgcttc
     2281 catcagtcgt tgctggattt gcgccaggcc gccgctgacg gtcgggataa tctgataacc
     2341 attaaattca cgaataccgg ttattgcagg cgtatttgga tagaaatcga gatcaacata
     2401 cagtgcgcca gtgaccaggt ttccggtttt cagcgatccg cgtaaaccac gtttcaacaa
     2461 ttcgccaagg tgctcaacaa catccgcatt ttcgccaagc tgcattttca gccgctctgg
     2521 ctcgatacga atcagtaccg gaatacggta atcatcgtta aatgtctgac gcatattcgg
     2581 cgcaaagaat ggcactttgc ttacggtacc caggcgaata ccccggaatt ctaccggagc
     2641 tcccggttgc agaccgcgta ccgaatcttt aaagaacatc agataatcaa tgtgatcggt
     2701 gtacaacgaa tcctgaatgc tcttctgatc atcatacaaa acgaaagctg ttttcggtgc
     2761 cactggctgc cctaaatcca gaccttccgg cacatcaaag ctgacaccgc cactaagcag
     2821 cgttgtcaat gagcccatct ccacacgcat tcccgctgac gtcagatcaa ccgcaatgcc
     2881 actatctttc cagaaacgaa cattgctggt caccagtcgg tcataaggtg cattgatgaa
     2941 cagttgatag ctgatattgc gtttttgcgt atcgaaagtg ctggtttcaa ccgaacctac
     3001 ccgatagcca cggaacagca ccggatcccc tggcgagagc tgcccggctt ttttgctatc
     3061 gagaagcaca cggatacctt tcgcatcagg cggggccaac ggtggcgagt ccagcaaatc
     3121 gtatttatcc attttgctgc ctttcgcgcc tggctgcagt tcgatataaa ccccagacag
     3181 cagcgttccc aggccgctaa tcccttctcg accaatctgc ggtttcacca cccaaaagac
     3241 ggtatcttta tgcagcaatt tttccatacc ggaattcagc cgcgctttga tttcaacgtg
     3301 cgtcaaatca tcagccagtg tggcgctttc aaccacgccg acgtcaacgc tacggctttt
     3361 aatggtggtt ttgccacctt caattccttc cgcattcgcg gtgatcaggg tcacttccgg
     3421 tccctgatgg ctgtaatgat aaaaaagaac ccaggcccca atgagcgccg tgacgatagg
     3481 aaatatccac acgggagacc agttcttcac tttctggatt ttggcttccc cattattaga
     3541 ttccatactg tcaggactcc tcatgctctg attcaggttg acgatcccac gacaaacgcg
     3601 ggtcaaacgt catagcagaa aacattgtca ttatgacgac taaagcaaac attaatgcac
     3661 ccattgccgg ataaatactc attaaacctc ccatacgcac cagcgccgag agcaccgcga
     3721 taacgaaaac gtcaatcatc gaccagcggc ctacaaactc aacaacttca taaatcaaat
     3781 gcattctttc actgtcgcgc ttgccgtgtc ctttggcatc ccaacacagc cacgcaatgg
     3841 cgatcatctt taacgttggc accataatac tggccagaaa aatcaccgca gcgacgggat
     3901 aagagccttc gctccataac agaatgaccc cagcgagaat cgtcgacggc atcttcgagc
     3961 ctaataaatc cgtcaccatg atgggcaaaa tattagccgg aagatacagc atgatagacg
     4021 ttacaagcag cgcgagtgtc cattgcaggc tgtttctacg ccgaacgtac cctttggtac
     4081 tacaacgcgg gcacacgggt tcatcagcag gaaggattgc cgtacaacag gaacacgaac
     4141 gcagcccctg gcgtatcccc gtgacgcctg gttttagcgg ctggcgcagt tctggcatcg
     4201 gggcgatgtc gtcccataac cagcgacgat caacgcactg aaaagcgcgc agttgcagga
     4261 cacaaaataa acaccagggg agaaagctgc tgcctacccc aatgctgccg taagccatca
     4321 gtttaacgaa actgaccagc acacccgcga ggaaaatctc cgccattccc caggttttga
     4381 gttgaaaaag cacccgtgcc agttgctctt ttaaacggac cggtaattcc gcgcgattca
     4441 ccagtaacag aatggttatc agacaaaacg cgggaaccag ttgcacaaac aacaggaaaa
     4501 aggtgccgag gctggcgtag tcctcagaaa aaagcacgcc gggaatttcc agtaatgtaa
     4561 tttcactggt aactcctgca acgttcatat tcacaaaagg aaacaagttg gacagcaaca
     4621 gcatgaacag tgcagccaac gcataggcgg tgggacgctg ccgaggggca tcccacgcca
     4681 cggttaacgt tgtgccacac cggggacatg ccgctttctg accatgctca aggcgcggta
     4741 acgccaccag catgtcacac tgcgagcaca ggatgtgctt cgcggcatga tgatgttcgc
     4801 acattggtac tccttaatga tgcgctattt tgcagatgac taacaaagtg ttggttttca
     4861 tgcctgataa acccagtaca tcaggcaatt ttaccgttgt cagcagtctc tgtttaaatc
     4921 gactattttg cgatcagcca ccatttttta acgcttcaag atactcccag cgttcaaagg
     4981 cttgctccag ctcctgctct gcagcagcca tatcagcgag tactttttgc gtctgctcat
     5041 gcggctgact gaagaaggaa gcatccgcca cttgcgtctg taaagcttcc agcttcgcct
     5101 ccagatcttc aagcaattgc ggtagcagct ccagttcacg ctgcaattta tagcttagtt
     5161 tgctactgct gcgttttaca gtttctgctt ttggcgcggc ggcttcttcg ctttttttca
     5221 ccgcaggctg tttgagcgcc acatactgtt cttgctgacc acgggcatca tgataaccgc
     5281 cgacataacg accaatttta ccgccgcctt cgaagatcca gcattctgta acggtgttat
     5341 cgacaaactg acgatcgtgg ctaaccagca ataccgtgcc ctgatagctg tcgatcagtt
     5401 cttccagcag ttccagcgtt tcgacatcaa gatcgttggt cggttcgtca agaatcaata
     5461 agttgcttgg tttgaggaac aaacgcgcca acaacaagcg gttacgctca ccgccagaaa
     5521 gcgcacgtac cggcgtcatc gcccgttttg gatggaacag gaagtcctgc aaatagccca
     5581 atacgtggcg tggcttgccg ttaaccatca cctcttgctt accttcggca aggttatcca
     5641 tcaccgtttt atcgggatcc agttccgcgc ggtgctgatc gaaataagcc acttccagtt
     5701 tggtgccaac gtgaatacgc ccgctgtctg cttgaagctg accgagcatt agtttcagca
     5761 gcgtggtttt gccgcaccca ttcggaccaa tcagggcaat tttgtcgccg cgtagaacct
     5821 gggcagaaaa atctttcacc agttgcttac cgtcaacctg gtagcaaacg tcctccattt
     5881 cgaaaacgat cttaccggag cggctggctt cttccacctg catctttgcg gtacccatca
     5941 cttcgcgacg ttcaccacgc tcacgacgca ttgctttcag ggcgcgtacg cggccttcat
     6001 tacgggtacg acgtgctttg atcccctggc ggatccacac ctcttcctgc gccagtttgc
     6061 gatcgaactc ggcattttgt aattcttcca cgcgcagggc ttcttctttt tccagcaggt
     6121 actggtcgta attccctgga taggtcacca gcttgccgcg atcgagatca acaatgcgcg
     6181 tcgccatatt gcggataaac gaacggtcgt gggaaatgaa aataattgtc ccattgaagg
     6241 ttttgaggaa cccttccagc cagtcgatgg tttcaatatc caggtggtta gtcggttcgt
     6301 cgagcagcaa tacgcgcgga ttgctcacca gcgcgcgtcc taatgccgct ttacgcaacc
     6361 agccaccgga aagcgacgac agcgcaacat taggatctaa tcccagttgc gccagcactt
     6421 cgttgatgcg gttttccagc tgccacaggt tgtggtgatc cagctgttcc tgaaccttcg
     6481 ccagttcgtt gagatttttc tcgctcgggt cgttcatcac caggcgcgaa atatcgtgat
     6541 agcgtttcag gtattccgct tgttcttcaa tgccttcggc aacgaaatca taaacgctac
     6601 cctcaacgtt acgcggcgga tcctgttgca gacgagctac aatcaaatct tgctcgtaaa
     6661 taatgcgacc gtcatccagc ccctgttcac ggttgaggat cttcattaac gtcgatttgc
     6721 ctgcgccgtt acgacccacc agacaaacac gttcgttatc ttcgatatgc agttctgcgt
     6781 tatcgagaag cggcgcgtcg ctgaacgaca gccatgcgcc atgcatactg attaatgaca
     6841 ttactattcc tttcaggctg cggtaatcag ccagcagttg tggatctggc ggttacgggc
     6901 gaaatcctgg gagagcgttt tttgcgtaat ttcttgtgct ttcagtccca gtttcgccag
     6961 gccgtcgaga tccatacgga agccgcgttt gttgttcgag aacatgatcg tcccacctgc
     7021 acgcagcaga cgtttcaaat ctttcatcag cgccagatga tcgcgctgaa catcaaacgc
     7081 atcttccatt cgttttgagt tagagaaggt tggcggatcg ataaagatca gatcgaactg
     7141 ttcatttgcc tcacgcagcc acgccaggca atcggcctga atcaggcgat gcgcacgccc
     7201 ggttaggcca ttcagacgca ggttgcgttc tgcccactcc agataagtac gcgacatatc
     7261 cacggtggtg gtgctgcgcg cgccgcctaa tcccgcatgc acggtggcgc taccggtata
     7321 ggagaacagg ttgaggaaat ctttgccttt gctcatctga ccgagcatac gacgggcgat
     7381 acggtgatcg aggaacagac cggtatcgag gtaatccgtc aagttcaccc acaagtgagc
     7441 gttatattcg gtaacttcaa gaaactcgcc cttctcgccc agtttctggt actgattttt
     7501 gcccttctgg cgttcacggg ttttcagcac cagtttgttt ggcgcaatcc ccagtaccga
     7561 aatggttgca gcgataatat cgaacagacg ctgacgcgct ttgtgcgcat caatagtttt
     7621 tggcggcgca tactcctgca ccaccaccca gtcggcataa cggtcaacgg caacgttata
     7681 ttccggcaga tcggcatcat acaggcgata acattcaatc ccttcctgac gtgcccactt
     7741 ctcgaatttt ttgaggttct tacgcaggcg gttggcgtag tcttccgcta ccattgccgg
     7801 tttgctgtct ggtgtgcttt cggcaacgtg gtagtttttc tgtacgcagt ccagcgggcc
     7861 gtttttcgcc ttgtactgtt tgtctgcacg cagctgcagg cagcttagca gatccggcga
     7921 ggcactaaac aaagagagat tccagccacc gaactggttt ttcatgattc ggcccagcag
     7981 actatgcagc gcaatcagcg ccggttcgct gtccagacgt tcaccgtatg gcgggttgct
     8041 caacactgta ccgtacggcc ctttcggcag cggattggtc agttgcgcga catctttcac
     8101 ctcaaaggtg atcagttcac caatccccgc aagacgggcg ttagtgcgcg cacgttgaat
     8161 cacccgggcg tcgctgtccg aaccgtaaaa atgagagcta tactctgcca ggcctttacg
     8221 ggcgcgagtt tgcgcttccg ctttcacttc ctgccagata gcttcatcat gctgcgccca
     8281 gccgctaaag ccccaacggc cacggtgcaa gcccggtgcg cggtcggtcg ccagcatcgc
     8341 tgcttcaatc agcaacgtac cagaaccaca cattggatcg agcagtggtg ttcctggctg
     8401 ccagccggat cgcatcacaa tcgcggctgc cagggtttct ttgatcggtg caataccagc
     8461 acgatcacga tagccacgca gatgcaagcc atcaccactg agatcaaggg cgatattggc
     8521 ggtttcttta tgcagccaga cgttaacgcg aatatccggc gcatcgcgat caacattggg
     8581 acgtggcaga tttttccgcg tgaaagcatc gacgatcgcg tctttcactt tcatcgcgcc
     8641 gtactggctg ttgcgaatgg tgtcatttaa accactgaaa tggacagcga aggtcgcgcc
     8701 aggattaaac atctctgtcc agttgatcgc ctgaacgccg agatagaggt ctaagtcact
     8761 gtaaacctta cactcgccca gcggcaacat aatacgcgag gccaggcggc tccacatcag
     8821 gctctggtaa acaagccgtg tgtcgccctt gaaatggacc ccaccctgaa ccacctggca
     8881 ttcaacggcc cccaggtttt ccagttcagt ttttaacagc tcttccagcc cacgggccgt
     8941 actggcaaac agagaattca tatcgtcact tttactctaa gaaaattgtt gcgcattata
     9001 gctaataagc gcgtcatgtc ataaagttga gggcttattt tcatttgagg accgcaccgt
     9061 ggcgacatta acccggcttt ttattcatcc tgttaaatcg atgcgcggca ttggtcttac
     9121 acataccctg gcagatgtca gtggtctggc cttcgatcgc atctttatga tcacggaacc
     9181 tgacggtacg tttattaccg ctcgccagtt tccgctaatg gtacggttta ctccttcacc
     9241 cgtgcatgat ggcttgcatc tcaccgcacc agatggcagt agcgcatatg ttcgttttgc
     9301 tgatttcgcc acacaagacg caccaaccga agtttggggc acacatttta ccgcgcgaat
     9361 tgcgccagat gcgatcaaca aatggctaag tggatttttc tcccgcgaag tgcaattacg
     9421 ctgggtgggg ccacaaatga cccggcgcgt gaaacgccac aacactgtac ccctgtcatt
     9481 tgctgatggc tatccttacc ttcttgctaa cgaggcctcg ttacgtgctc tccaacaacg
     9541 ttgtccggcc agtgtaaaaa tggagcaatt ccgccccaat ctggtggttt ccggtgcatc
     9601 ggcctgggaa gaagatagct ggaaagtgat tcgcattggt gatgtcgtgt ttgatgtggt
     9661 taagccttgt agccgctgta ttttcaccac cgtcagccca gaaaaagggc aaaaacatcc
     9721 ggcagacgaa ccattaaaaa cgctgcaatc tttccgtact gcccaggata acggcgatgt
     9781 cgattttggt cagaatttaa ttgcccgtaa tagcggcgtg attcgcgttg gcgatgaggt
     9841 ggaaattctg gcaacggctc cagctaaaat ttacggcgca ggtgccgctg atgatactgc
     9901 caacatcacg caacaaccgg acgcaaacgt agatattgac tggcagggac aggcatttcg
     9961 tggaaataac caacaggtgc tgctggagca attagaaaat cagggaattc gtatccctta
    10021 ttcttgccgc gcaggcattt gtggaagttg ccgtgttcag cttttagaag gcgaagtcac
    10081 gccgctaaaa aaatcagcaa caggcgatga tggcactatt ctttgctgta gttgtgtacc
    10141 gaagactgcg cttaagctgg cgcgttagac tgcctgttcg aggctgaagc tgtcaacatt
    10201 aacctgcggt ttcagcctgt cgttcatgat tttaatggcg tcgcccaact gcatcgcgcg
    10261 acccgcaata acaacgcagg gttgtgccag tagacatagc gcggcattct cgccagattc
    10321 aacgaccagt aaattcactt gctcgtgagt gtcgctcaac caaacgtagg ccgcatcgcc
    10381 cggcatgggc atccacattt caccatgaga tacaaaatgc cagcttttcg gcatttgcgg
    10441 tttgagataa cgaattgcta ccagtgcatt cagcaccagt tcggcttttt gttctttaga
    10501 taaattaaaa tcgcggcact tttcttcaaa agagaaatag agcgcggcgt catcaacgca
    10561 aaaacctgcg ggagaaaagg catctggagt caacattttg cgcgcaaaac gtgagcgaaa
    10621 tagcatacca ttggctaaat cgagcatcat tcgatcgtgc tcttcatcgt aataccaacg
    10681 ccagttatcg tctggtttaa ttcgcatctg ccactcccca accttataaa accgcagcga
    10741 cacaatccat tgtcgcttcg cttaataacc tcaacaataa aagtacaaaa tgtctaaata
    10801 agcaacagcg atgaatataa aacaaccagg g
//
LOCUS       DAHHTG010000137        10628 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.138, whole genome shotgun sequence.
ACCESSION   DAHHTG010000137 DAHHTG010000000
VERSION     DAHHTG010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10628)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10628)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10628
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.138"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            257..757
                     /gene="tssB"
                     /locus_tag="NBI74_003763"
     CDS             257..757
                     /gene="tssB"
                     /locus_tag="NBI74_003763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001557371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     small subunit"
                     /protein_id="HCD1934038.1"
                     /translation="MADSFQNEVPAARVNIKLDLHTGNAKKKVELPLKLLAVGDYSNG
                     KEQRPLSERDKIDINKNNFNSVMAEFSPAVNLTVEDTLNGNGNEQNIALEFKSLKDFE
                     PEQVAKNIPQLRVLLAMRNLLRDLKSNLLDNATFRRELENILKDPTLSSELRDELAKI
                     APQENV"
     gene            816..2354
                     /gene="tssC"
                     /locus_tag="NBI74_003764"
     CDS             816..2354
                     /gene="tssC"
                     /locus_tag="NBI74_003764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001314091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     large subunit"
                     /protein_id="HCD1934039.1"
                     /translation="MSVQQEHATSETATLTTTESGGVYQSLFDKINLTPVSSIQEIDS
                     WQNSETLADASPDERVTAAIHVLLSCLAKSGEDVVKLDKSLLDFHIDDLDQKISKQLD
                     AVMHHPEFQKVESLWRGTWFVVQRTDFRKNVRIELLDVSKEHLRQDFDDSPEIIQSGL
                     YRHTYIQEYDTPGGEPVASLISSYEFDNSPQDIALLRNISRVSAASHMPFIGSVGPKF
                     FLKNSMEEVAAIKDIGNYFDRAEYIKWKSFRDTDDSRYVGLVMPRVLGRLPYGPDTVP
                     VRSFNYVEEVKGPDHEKYLWTNASFAFAANMVKSFVNNGWCVQIRGPQAGGAVADLPI
                     HLYDLGTGNQVKIPSEVMIPETREFEFANLGFIPLSYYKNRDYACFFSANSAQKPALY
                     DTADATANSRINARLPYIFLLSRIAHYLKIIQRENIGTTKDRRVLELELNTWIRTLVT
                     EMTDPGDELQASHPLRDGKVIVEDIEDNPGFFRVRLFAVPHFQIEGMDINLSLVSQMP
                     KAKA"
     gene            2372..3709
                     /gene="tssK"
                     /locus_tag="NBI74_003765"
     CDS             2372..3709
                     /gene="tssK"
                     /locus_tag="NBI74_003765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssK"
                     /protein_id="HCD1934040.1"
                     /translation="MKIHRPLWAEGTFLSSQQFQQQARWEAFSNDSIAQLCIRHPWGI
                     ANVLFDRDALTSGKLKTQAVRLRFADGTLIDSDVSDVLPLACDLRALKNDSAIVLLAL
                     PLAHGNGGNLGQGEQTERPLRYRQEWQKVQDIYGSDSEDMAVERHALSLRFAHDNNQD
                     YITCPLARLVRDVQGNWTQDESYIPPLLAFNAHDGLVQRLDTLLLQLRAKCQRLMAMR
                     RESNQRMADFAVADVSLFWLLNALNSAEPVLSDFLRYPAVHPELVWRELARLAGALLT
                     FSLEHNVSAVPPYVHESPSIVFPPLFSLLSELLEASLPSRVIALDLASLPGNRWKADL
                     HDPRLREEADFYLSVRSSLPAHQVLHQLPLVCKIGAPDDVTLLINVALNGVPLVPLTS
                     VPAALPLRLENQYFALDMHSDAAKSMLESGCCMIYAPGTMGDLKPELFAVLRT"
     gene            3706..4371
                     /gene="tssL"
                     /locus_tag="NBI74_003766"
     CDS             3706..4371
                     /gene="tssL"
                     /locus_tag="NBI74_003766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system protein TssL, short
                     form"
                     /protein_id="HCD1934041.1"
                     /translation="MKKETDINIDALLRDTFLTVVELRQGTTVRHGMELYRHCQRQVE
                     LVRERLKDAGFSRESVEHITYAQCALLDETVLSRGGMDDGQAIWMKDPLQSHFFNTLQ
                     AGELLYERMKQVLQEPAPAQAVLTCFHRVLLLGFRGRYQDPAAPERDQLISTLNGQVA
                     PFGVLPETAVLNVPLSPRQHPLLHSPFFWLVTLTLVLAGVWWGLHYWLNVLVDELLPQ
                     SLR"
     gene            4384..6036
                     /locus_tag="NBI74_003767"
     CDS             4384..6036
                     /locus_tag="NBI74_003767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001246525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HCD1934042.1"
                     /translation="MRNALKQTVLLWTLLLLLTTWLGFFAVSAVVKYAVVVILVAVTA
                     AVMAYWHKKKGQVVADDVWPSSLPPETYRQPVVLVCGDMSAHLFTDSPVRQVSEGLYL
                     PVSDEEQLVAQVERLLTLRPAWASQFAVAYTVMPGMYRDAAVLTGQLRRFAHSMATVR
                     RRAGVNVPWLLWSGLSGSPLPERANSPWFICTGGEVQVATSAETTMPAQWIAQSGAQE
                     RSQRLCYLLKAESLMQWLDLNVLAELNGPEAKCPPLAMTVGLVPSLPAVDNNLWQLWI
                     TARTGLTPDIADTGTDDALPFPDALLRRLPRQSGFTPLRRACVTMLGVTTVAGIAALC
                     LSATANRQLLRQVGDDLHRFYAVPAEEFITKARHLSVLKDDAVMLDGYYREGEPLRLG
                     LGLYPGERIRQPVLRAIRDWRPPEQKMDVTASLPVQTVRLDSMSLFDVGQARLKDGST
                     KVLVDALVNIRAKPGWLILVAGYTDATGDEKSNQQLSLRRAEAVRNWMLQTSDIPATC
                     FAVQGLGESQPAATNDTPQGRAVNRRVEISLVPRSDACQDVK"
     gene            6094..6585
                     /locus_tag="NBI74_003768"
     CDS             6094..6585
                     /locus_tag="NBI74_003768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hcp family type VI secretion system effector"
                     /protein_id="HCD1934043.1"
                     /translation="MAIPAYLWLKDDGGADIKGSVDVQGREGSIEVVALDHDVYIPTD
                     NNTGKLTGTRTHKPFTFTKEIDASSPYLYKAVTTGQTLKTAEFKFYRINDAGQEVEYF
                     NITLDNVKLVRVAPLMHDIKDPSREKHNHLERIEFRYEKITWTYKDGNIIHSDSWNER
                     PSA"
     gene            6776..9412
                     /gene="tssH"
                     /locus_tag="NBI74_003769"
     CDS             6776..9412
                     /gene="tssH"
                     /locus_tag="NBI74_003769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001520117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system ATPase TssH"
                     /protein_id="HCD1934044.1"
                     /translation="MTGNHPAALLRRLNPYCARALDAAASLCQTRAHAEITIEHWLLK
                     LLEQGEGDITVIARRYEWDIDTLWQSLLAHLDTLPRPVRERPQLSEPLAALIRQAWLI
                     ASLEGDDPQIRSQHLLMALTEKPMLPACNDLWVLLSLSRVQLERLRPLLDAQSDECPA
                     RQPQVTEPLTSAQPGAATTDAPAKTLTGKQDDALLAVLNRFTEDVTEKARSGRIDPVF
                     GRDTEIRQMVDILSRRRKNNPILVGEPGVGKTALVEGLALRITEGNVPDSLKTVHIRT
                     LDLGLLQAGAGVKGEFEQRLKNVIDAVQKSPEPVLLFIDEAHTIIGAGNQAGGADAAN
                     LLKPALARGELRTIAATTWSEYKQYFERDAALERRFQMVKVDEPDDDTACLMLRGLKA
                     RYAQHHGVHMLDSAIQTAVRLSRRYLTGRQLPDKAVDLLDTAGARVRMSLDTLPEPLT
                     QLHARLAALDIEREAIEQDSVFYPETSPERLAELTDLRDELQAEAGHLEAQYQQEKAL
                     AQQIMTLRQEGTDSSELQQQLRTHQGFAPLLALDVDARAVATVVADWTGIPLSSLLRD
                     EQSDLLSMEQSLENRVVGQSPALCAIAQRLRAAKTGLTPENGPQGVFLLTGPSGTGKT
                     ETALTLADTLFGGEKSLITINLSEYQEPHTVSQLKGSPPGYVGYGQGGVLTEAVRKRP
                     YSVVLLDEVEKAHRDVMNLFYQVFDRGFMRDGEGRVIDFRNTVILMTANLGSDHIMQL
                     LEEKPDATDADLHELLYPLLRDHFQPALMARFQTVIYRPLGQEAMRTIVEMKLAQVVR
                     RLHQHYGLETEINDSLYDQLTAACLLPDTGARNIDSLLNQQILPVLSQQLLAQQAVHH
                     KPARLRLGWDEEDGIVLEFDEK"
     gene            9424..>10628
                     /gene="vgrG"
                     /locus_tag="NBI74_003770"
     CDS             9424..>10628
                     /gene="vgrG"
                     /locus_tag="NBI74_003770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001574080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
                     /protein_id="HCD1934045.1"
                     /translation="MNVQFFDHAHHKLKIRGLKSPVDVLTFTGHEQLSSPFRYDIEFT
                     STDKAIEPESVLMQDGAFSLSAPPVQGMPVQVPLRTLHGVITSFKHLSSSQDEARYEV
                     RLEPRMALLTRSRQNAIYQNQTVPQIVEKILRERHQMRGQDFVFNLKNEYPAREQVMQ
                     YGEDDLTFVSRLLSEVGIWFRFATDARLKIEVVEFYDDQSGYERGLTLPLRHPSGLFD
                     GETEAVWGLNSAYSVVEKSVSTRDYNYREATAEMTTGQHDATGGDNTTYGEAYHYADN
                     FLQKGDKEAAESGAFYARIRHERYLNEQAILQGQSTSSLLMPGLEIKVQGDDAPAVFR
                     KGVLITGVTASAARDRSYELTFTAIPYSERYGYRPALIPCPVMAGTLPARVTSTVKND
                     IYAHIDKDGR"
BASE COUNT         2409 a         2982 c         3039 g         2198 t
ORIGIN      
        1 aatttgtgaa gcttatcctg ttattatttt aagtttcatg ccaaattctc tcaccagata
       61 atgccgccct ctcccgaaaa ataatcaaga ggccaaacaa tatctaaaat gatacaactg
      121 tatctattcc cctgaaaaat acattattca tttgtatatt ttcctcatca ttgcttttta
      181 tttaaatcat ccgataatcc cctgaatata attatgtcaa taaccatcag aaaaagtgga
      241 tgatgaggaa aaggatatgg ccgacagttt ccagaatgaa gttcccgccg ctcgtgtaaa
      301 tatcaagctt gacctgcata caggcaatgc taaaaagaaa gttgaactcc ccctcaagct
      361 tcttgccgta ggcgattaca gtaacggaaa agagcagcgt ccgctgtccg aacgggacaa
      421 gattgatatc aataaaaaca acttcaacag cgtcatggct gagttttcgc ctgcggttaa
      481 tttaacagta gaagatacgc taaacggaaa cggtaatgaa caaaatatcg cgcttgaatt
      541 taaaagcctg aaggattttg agcccgaaca ggtagcaaaa aatattccac aacttcgtgt
      601 attactcgct atgcgcaatt tactacgtga cctgaaatcc aatctgctgg ataacgcaac
      661 gtttcgccgt gaactggaaa atatcctgaa agacccgacg ctcagttcag aactgcgtga
      721 tgaactggcg aaaattgcgc cgcaggagaa cgtttaaccc gaacgcgtat tcaggttaaa
      781 ccggagaaga aaggatcact gaaaaggaaa tgctgatgtc tgtacaacaa gaacatgcca
      841 cctctgaaac tgcaacactc accaccactg agtccggcgg cgtttatcag tccctgtttg
      901 ataaaattaa tttaaccccg gtctcttcca ttcaggaaat cgattcatgg caaaacagcg
      961 aaacgctggc cgatgcctca cccgatgagc gcgtgacggc ggctatccac gttctgcttt
     1021 cctgtctggc gaagtctggc gaggacgtgg ttaaactcga caaaagcctg ctggattttc
     1081 atatcgacga tctggatcag aaaatcagta aacagcttga tgcagtcatg caccatccgg
     1141 aattccagaa agtcgagtcg ctctggcgcg gcacatggtt cgtcgtacag cgcactgatt
     1201 ttcgcaaaaa tgtcagaatt gaactgctgg atgtcagtaa agaacatctg cggcaggact
     1261 ttgatgattc tccggaaatc attcaaagtg gtttatatcg ccatacatac attcaggagt
     1321 acgatacgcc gggtggcgaa cctgtagcct cattaatatc cagctatgaa ttcgataaca
     1381 gcccgcagga tattgccctg ctgcgtaata tttccagagt ctctgctgca tcacatatgc
     1441 cctttatcgg ttccgtcgga ccgaagttct tccttaaaaa ctcgatggaa gaagtcgccg
     1501 caattaaaga tatcggcaac tattttgacc gcgcggaata tatcaaatgg aagtcgttcc
     1561 gcgataccga cgacagtcgc tacgtgggat tagtgatgcc gcgcgtgctg ggccgtctgc
     1621 cctatgggcc ggacacagtg ccggtacgca gctttaacta tgtggaagaa gtcaaaggcc
     1681 cggatcacga aaaatacctc tggacaaacg cctcgttcgc ctttgccgcc aatatggtga
     1741 agagctttgt gaataatggc tggtgcgtgc agatccgtgg tccgcaggcg ggtggcgcag
     1801 tggccgatct gccgatccat ctttacgatc tcggcaccgg caatcaggtc aaaattccgt
     1861 ccgaagtgat gatcccggaa acccgcgaat ttgaatttgc caaccttggc tttattccgc
     1921 tctcttatta taagaatcgc gattacgcct gcttcttctc agcgaactct gcccagaaac
     1981 cggcgttgta tgataccgct gacgccaccg ccaacagtcg tattaacgcc cgtttgccct
     2041 atattttcct gctgtcccgc attgcgcatt acctgaaaat catccagcgc gagaatatcg
     2101 gtaccaccaa agaccgccgc gtactggaac tggagcttaa cacctggatc cgcacactgg
     2161 tgacggagat gaccgatccg ggtgatgaac tgcaggcgtc tcacccgctg cgcgacggca
     2221 aggttatcgt ggaagatatt gaggacaatc cgggcttctt ccgcgtcaga ctctttgccg
     2281 tgccgcattt ccagattgaa gggatggata tcaacctgtc actggtttcc cagatgccga
     2341 aagcgaaagc ctgaccgggc cgtggtgtga tatgaaaatt catcgaccac tgtgggctga
     2401 ggggacattc ctgtcctctc agcagtttca gcagcaggcc cgctgggaag cgttcagcaa
     2461 tgacagcatt gcgcaacttt gtattcgtca tccgtggggg atcgcgaatg tgctctttga
     2521 ccgcgatgcc ctgacgtccg gaaagctgaa aacgcaggct gtacgtctgc gttttgctga
     2581 tggcacgctg atcgattcag acgtgagtga cgttctgccg ctcgcctgcg atctgcgcgc
     2641 gctgaagaac gacagcgcga ttgtcctgct ggcgctgccg cttgcgcatg gtaacggcgg
     2701 taatctcggt cagggtgagc agactgaaag accgctgcgc tatcgtcagg aatggcaaaa
     2761 ggtgcaggat atctacggca gtgacagtga agatatggcc gttgagcgtc atgcgctgtc
     2821 cctgcgcttt gctcatgata acaatcagga ttacatcacc tgcccactgg cgcgtctggt
     2881 gcgggatgtt cagggcaact ggacacagga tgagagctat attccgccgt tactggcgtt
     2941 taacgcccat gacgggctgg tacagcgtct ggacacgctg ctgttgcagc tacgggcgaa
     3001 gtgccagcgt ctgatggcga tgcgccggga aagcaaccag cgtatggcgg acttcgctgt
     3061 cgccgatgtc tccctgttct ggctgcttaa cgcgctgaac tccgccgagc cggtactgag
     3121 tgatttcctg cgctacccgg cggttcatcc cgaactggtc tggcgggagc tggcgaggct
     3181 tgccggtgcg ctgctgacgt tttcccttga acataacgtc agcgccgttc cgccctatgt
     3241 ccacgaatcg ccgtccatcg tctttccgcc acttttctcc ctgctcagcg aactgctgga
     3301 agccagcctg ccgtcacggg tgatcgcgct ggatctggcg tccctgccgg gcaaccgctg
     3361 gaaagcagac ctgcacgatc cgcgcctgcg ggaagaggcc gacttttacc tgtccgttcg
     3421 ttcctctctg cctgcgcatc aggtactgca ccagctaccg ctggtctgca aaatcggtgc
     3481 gcctgatgac gtcacgctgc ttatcaatgt ggcgctgaat ggtgttccac tggtgccgct
     3541 tacctcagtt ccggcagcgc tgccactgcg gctggagaac cagtattttg cgctggatat
     3601 gcacagtgac gcggctaaat cgatgctgga gtccgggtgc tgcatgattt atgcgccggg
     3661 aacaatgggc gacctgaaac ctgaactgtt tgcggtgctg cgcacatgaa aaaagagacg
     3721 gatatcaata tcgacgcgct gctgcgcgac acgtttctga cggtggtgga actgcgtcag
     3781 ggaaccaccg tgcgtcacgg tatggagctg tatcgccact gccagcggca ggtggaactg
     3841 gtccgcgaac gcctgaaaga tgccgggttc agccgggaga gcgtggagca cattacttac
     3901 gcgcagtgcg ccctgctgga cgaaacggtc ctgagccgtg gcggcatgga cgacggtcag
     3961 gccatctgga tgaaggatcc actccagtcg catttcttca acactctgca ggccggtgag
     4021 ctgctgtacg agcggatgaa gcaggtgcta caggagcccg cccccgcgca ggccgtgctg
     4081 acctgctttc accgtgtcct gctgctggga tttcgcgggc gttatcagga tccggcagcc
     4141 ccggagcgcg accagcttat ttcgacgctc aacgggcagg tcgcgccatt tggtgtgctg
     4201 ccggaaacgg cggtactgaa cgtgccgtta tccccccgtc agcacccttt gctgcattcg
     4261 ccgtttttct ggctggtgac gctcacactg gtactggccg gtgtctggtg ggggctacat
     4321 tactggctga acgtgctggt ggatgaactg ctgccgcaaa gtctgcgtta aagggagtcg
     4381 tgagtgagga atgcgctgaa acagacggtt ttgttatgga ctctgttgtt gctgctgact
     4441 acctggctgg gtttctttgc tgtatctgcg gtcgtgaagt atgccgtggt agtgattctt
     4501 gtcgccgtca cagcggcggt gatggcgtac tggcataaga aaaagggaca ggtcgtagcg
     4561 gacgacgtat ggccgtcatc gctaccgccg gaaacctacc gacagccggt agtgctggtc
     4621 tgtggcgata tgtcggcgca cctgttcact gacagcccgg tccggcaggt ttcagaaggg
     4681 ctgtatctgc ctgtttctga tgaagaacag cttgtggcgc aggtggagcg attactgacc
     4741 cttcgcccgg cgtgggcatc gcagtttgcc gtggcgtata ccgtcatgcc cggcatgtac
     4801 cgggatgcgg cggttctgac cggacaactg cgccggttcg ctcacagtat ggcgacggtg
     4861 cgtcgccggg cgggcgtaaa cgtaccctgg cttctctgga gcgggctgtc cggctcgccg
     4921 ttgccggaga gagcgaattc accgtggttt atctgtaccg gcggcgaagt tcaggtagca
     4981 acatccgcag agaccaccat gcccgcgcag tggattgcac aatccggcgc acaggaacgc
     5041 agtcagcgac tctgttacct gctgaaagcc gaaagcctga tgcagtggct ggatctgaat
     5101 gtgctggcgg aactgaacgg cccggaggcg aaatgtccac cactggcgat gaccgttggg
     5161 ctggtcccct cgttgcctgc ggtggataac aacctgtggc agttgtggat caccgccaga
     5221 accggcctga cgccggatat cgcggacacc ggcacagacg atgcgctgcc attcccggat
     5281 gccctgttac ggcggttgcc gcgtcagtcg ggctttaccc cgctgcgacg ggcctgcgtg
     5341 accatgctgg gcgtcaccac cgtggcgggt atcgccgcgc tgtgcctgtc agccacggca
     5401 aatcgccagt tattacggca ggtcggtgac gatctgcacc ggttttatgc cgtcccggcg
     5461 gaagaattta tcaccaaagc ccgtcacctg tcggtgctga aagacgatgc ggtcatgctc
     5521 gatgggtatt accgggaagg agaaccacta cgtctcggtc tggggttata ccccggtgaa
     5581 cgcatccgcc agccggtatt acgcgccatt cgcgactggc gtccgcctga gcaaaaaatg
     5641 gacgtgacgg cttcgcttcc ggttcagacc gtgcgtcttg acagtatgtc gctgtttgac
     5701 gtcggacagg cccgcctgaa agacggctcg acaaaagtgc tggtggatgc gctggtgaac
     5761 atccgggcaa aaccgggctg gctgatcctc gtggccggat ataccgatgc caccggcgat
     5821 gaaaaaagca atcagcagtt atcgctgcgg cgtgccgaag cggtgcgcaa ctggatgctg
     5881 cagaccagcg acatcccggc cacctgtttt gccgtacagg gactgggcga gagccagcct
     5941 gcggcgacca acgacacgcc acagggccgg gcagtcaacc ggcgtgtcga aatcagtctt
     6001 gttccgcgtt ctgacgcctg tcaggacgtg aaataaaaca taccgccgga agaaggcggt
     6061 gcttcaatca cactaacaag gagagtaatt ctcatggcta ttcctgctta tctctggctg
     6121 aaagatgacg gcggcgcgga tatcaaaggt tccgtggacg ttcaggggcg cgaaggtagc
     6181 atcgaagtgg tggcgctgga tcacgatgtg tacatcccga ccgacaacaa caccggcaaa
     6241 ctgaccggta cccgtactca caagcccttt acgtttacca aagaaatcga tgcctccagc
     6301 ccgtatctct acaaagctgt gaccaccggc cagactctga aaacggcaga atttaagttt
     6361 taccgcatca acgatgccgg tcaggaagtg gagtacttca acatcacgct tgataacgtc
     6421 aagctggtca gagtcgctcc gcttatgcac gacatcaagg atccttccag agagaagcat
     6481 aaccacctgg aacgtattga gttccgctac gagaaaatca cctggactta caaagacggc
     6541 aacatcattc attccgactc gtggaatgag cgtccttccg cctgataact gcggcagaca
     6601 ggaaactgtc tgcctttttg ttgtgtgctt tgtccagtgc cgcaggcacc ggtactgagc
     6661 aaagcatacc cacaatctgg tgaaacctga tgggcctgag tgaggtaacg gctatgggcg
     6721 gtagcgtgcc tgaacgcgga tcgcaggaat acagaacacg ataaggaaac cttctatgac
     6781 cggaaatcac cccgccgcgc tgctgcgtcg ccttaaccca tactgtgcac gggcgctgga
     6841 cgctgccgcc tcactgtgtc agacccgcgc ccatgcggaa ataaccattg aacactggct
     6901 gctgaaactg ctggagcagg gagaaggcga tatcacggtg attgcccgcc gctatgaatg
     6961 ggatatcgac acgctctggc agtctctgct ggcacatctg gacaccttac cccgcccggt
     7021 ccgcgaacgt cctcaacttt ctgaaccgct ggcagcgctt atccgacagg cgtggctgat
     7081 agcgtcactg gaaggcgacg atccacaaat ccgcagccag catctgctga tggcgctgac
     7141 agaaaaaccg atgctgcccg cctgtaatga cctgtgggta ttgctgagcc tgagccgtgt
     7201 gcagcttgag cggctgcgtc ccctgctgga tgcgcagtcg gatgaatgtc cggcacgtca
     7261 gccacaggtc accgaaccgc tgacatccgc gcaacccggg gcggcaacga cggacgcacc
     7321 ggcaaaaacg ctgacgggga aacaggatga cgccctgctg gcggtgctta accgctttac
     7381 cgaagacgtg acggaaaaag cccgcagcgg gcgaatcgac ccggtattcg ggcgcgacac
     7441 ggaaattcgc cagatggtcg atatcctctc ccgtcgccgc aaaaacaacc cgattctggt
     7501 gggagaaccg ggggtgggca aaaccgcgct ggtggaaggg ctggcgctgc gtatcaccga
     7561 aggcaacgtg ccggacagcc tgaaaacggt gcatatccgc acactggacc tcggtctgtt
     7621 acaggctggc gcgggcgtta aaggtgaatt tgaacagcgg ctgaaaaatg tcatcgatgc
     7681 agtgcagaaa tcaccggagc cggtactgct gtttattgat gaagcccata ccattatcgg
     7741 tgcgggtaat caggcaggcg gcgcggatgc ggcgaacctg ctgaaaccgg cgctggcaag
     7801 gggcgaactg cgcaccatcg cggcgaccac gtggagcgaa tacaaacagt attttgagcg
     7861 cgacgccgcg ctggaacgcc gcttccagat ggtgaaagtc gacgagccgg atgatgacac
     7921 cgcctgcctg atgctgcggg gactcaaggc ccgctatgca cagcaccacg gcgtacatat
     7981 gctggacagc gccattcaga ccgccgtgcg cctgtcgcgc cgctatctga ccggacgcca
     8041 gttgccggac aaagcggttg atttgctgga taccgccggg gcaagagtcc gcatgagcct
     8101 tgacactctg ccggagccgt tgacgcagct tcatgcgcga ctggcggcgc tggatatcga
     8161 gcgggaggcg attgagcagg acagcgtgtt ttatcccgaa accagcccgg agcggctggc
     8221 ggaactgacc gatttgcgtg atgagctaca ggcagaagcc gggcatctgg aagcgcagta
     8281 tcagcaggaa aaggcactgg cgcagcagat tatgacgttg cgtcaggaag gaacagacag
     8341 ctctgaactg caacagcaac tgcggacgca tcagggcttt gcaccgctgc tggcgctgga
     8401 tgtggacgcc cgcgccgtcg ccacggtggt ggcggactgg accggcatcc cgctctcttc
     8461 cctgctcagg gacgagcaga gcgacctgct cagtatggaa cagagtcttg aaaaccgcgt
     8521 tgtcgggcaa agcccggcgc tctgcgccat cgcacagcgg ctgcgggcgg ctaaaaccgg
     8581 cctcacgccg gagaacggcc cacagggggt attcctgctg accggcccca gcggcaccgg
     8641 caaaaccgaa actgcgctca cactggccga caccctgttt ggtggtgaaa aatcccttat
     8701 caccattaat ctttcggaat accaggaacc gcataccgtt tcccagttga aaggctcgcc
     8761 tccaggctac gttggttacg gtcagggtgg cgtacttacc gaagccgttc gcaaacgccc
     8821 ttacagcgtg gtgctgctcg acgaagtgga aaaggcgcat cgcgacgtga tgaacctgtt
     8881 ctatcaggtg ttcgaccggg gctttatgcg cgacggcgaa gggcgggtaa tcgacttccg
     8941 caacaccgtc attctgatga ccgccaatct gggcagcgac cacatcatgc aactgctgga
     9001 ggaaaaaccg gacgccacgg acgcagacct gcatgaactg ctgtatcccc ttctgcgaga
     9061 ccattttcag cctgcactga tggcgcgttt tcagacggtg atttaccgcc cgctgggaca
     9121 ggaggcgatg cgcaccattg tggaaatgaa actggcgcag gtggtccgcc gtcttcacca
     9181 gcactacgga ctggaaacgg aaatcaacga cagcctgtat gaccagctta ccgccgcctg
     9241 cctgctgccg gataccgggg cgcgcaatat cgacagcctc ctgaaccagc aaatcctgcc
     9301 ggtcttaagc cagcagttgc tggcacagca ggccgtgcat cataagcctg cccgactgcg
     9361 gcttggctgg gatgaggagg acgggattgt actggaattt gatgagaaat aatggaggct
     9421 cttatgaacg tacagttctt cgaccacgca caccacaagc tcaaaattcg cggcctgaaa
     9481 agcccggtgg acgtgctgac ttttacaggc cacgaacagc tcagctcacc cttccgctac
     9541 gacattgagt ttaccagtac cgataaagcc atcgagccgg agtcggtact gatgcaggac
     9601 ggcgcattca gcctgagcgc cccgccggta caggggatgc cggtgcaggt ccccctgcgc
     9661 accctgcacg gcgtcattac cagctttaaa cacctctcat cgtcacagga cgaagcccgt
     9721 tacgaagtgc gccttgaacc ccgtatggcg ctgcttaccc gcagccgcca gaacgccatc
     9781 taccagaacc agacggtgcc gcaaatcgtc gaaaaaatcc tgcgcgagcg ccatcaaatg
     9841 cgcggtcagg acttcgtgtt taacctcaaa aacgaatacc cggcccgtga acaggtgatg
     9901 cagtacggtg aggacgacct gacgtttgtc tcacgcctgc tgtcggaggt gggtatctgg
     9961 ttccgctttg ccaccgacgc ccggctgaaa atcgaagttg tcgagtttta tgacgaccag
    10021 agcggctatg agcgcggact gacgctgccg cttcgtcatc cttcagggct gtttgatggt
    10081 gaaacggaag ccgtatgggg gttgaacagc gcgtacagcg tggtggaaaa aagcgtctcc
    10141 acgcgggact acaactaccg tgaagccacg gcggaaatga ccacaggcca gcatgacgcc
    10201 accgggggcg ataacaccac ctacggcgaa gcctaccact acgctgacaa cttcctgcaa
    10261 aaaggcgata aagaggcagc ggaaagcggc gcgttttatg cccgtatccg ccacgaacgc
    10321 tatttgaatg aacaggccat tctacagggc cagagcacca gttcactgct gatgccgggg
    10381 ctggagataa aagttcaggg tgacgacgcg ccagcggtgt tccgcaaggg cgtactgatt
    10441 accggcgtga cggccagcgc ggcgcgtgac cgcagctatg aactgacgtt taccgccatc
    10501 ccgtactcag aacgctatgg ctaccgcccg gcgcttatcc cctgccccgt gatggccggc
    10561 acgctcccgg cacgggtcac cagcacggtg aagaacgaca tctacgccca tatcgacaag
    10621 gacgggcg
//
LOCUS       DAHHTG010000138        10600 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.139, whole genome shotgun sequence.
ACCESSION   DAHHTG010000138 DAHHTG010000000
VERSION     DAHHTG010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10600)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10600)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10600
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.139"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            20..355
                     /gene="iraM"
                     /locus_tag="NBI74_003771"
     CDS             20..355
                     /gene="iraM"
                     /locus_tag="NBI74_003771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraM"
                     /protein_id="HCD1934046.1"
                     /translation="MKWIVIDTVIQPSCGISFSVIWSKIKLIIWYQSDAFLPPESIFT
                     LTHTGIMLNNKVLPVTIYNVVPFNKTFWNLIKNSQECPTNTDNVLNECFNNRCTLQIC
                     PYGLKQQSP"
     gene            complement(636..749)
                     /locus_tag="NBI74_003772"
     CDS             complement(636..749)
                     /locus_tag="NBI74_003772"
                     /inference="COORDINATES: protein motif:HMM:NF024464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3927 family protein"
                     /protein_id="HCD1934047.1"
                     /translation="MLQTPSVLKAFLFVVNGRLVGVVVVAFPLLKKKTPDC"
     gene            complement(725..922)
                     /locus_tag="NBI74_003773"
     CDS             complement(725..922)
                     /locus_tag="NBI74_003773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309806.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrmB family transcriptional regulator"
                     /protein_id="HCD1934048.1"
                     /translation="MLKQQDMTEAARVVFNELSVTDPATVREIAQNTYLSRERCQLIL
                     TQLVMAGLADYQFGCYRHLQS"
     gene            complement(1135..1824)
                     /locus_tag="NBI74_003774"
     CDS             complement(1135..1824)
                     /locus_tag="NBI74_003774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001064894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator"
                     /protein_id="HCD1934049.1"
                     /translation="MNNQYLQFVREQLMIATADLSGSTKCQLEAWQENALFDTGRYRR
                     KKIRYRDEVTGKMITRDNPPIQGKQSLAKGSSIALVSPVEFATSSWRRAVLELEEHQK
                     AWLLWCYGGNICWEHQIAITQWGWSEFKTQSGSRKIAVKTLERVKKLIWLAAQDVRGW
                     VTGCEVYQRQELARLCGVKPDNWSHNYANYWREMCDIFKRLDRESLICSVKIRAQQKA
                     TFSRRDIAKVN"
     gene            complement(1817..2185)
                     /locus_tag="NBI74_003775"
     CDS             complement(1817..2185)
                     /locus_tag="NBI74_003775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000140038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HCD1934050.1"
                     /translation="MTERIEFVLPYSPTVNTYWRRRGSTYFVSKAGERYRRAVALIVR
                     QQRLKLSLSGRLAIKIIAEPPDKRRRDLDNILKAPLDALTHAGLLMDDEQFDEINIVR
                     GQPVSGGRLEIRITEVGVHE"
     gene            complement(2186..3244)
                     /locus_tag="NBI74_003776"
     CDS             complement(2186..3244)
                     /locus_tag="NBI74_003776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001265086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HCD1934051.1"
                     /translation="MRVLLRPVLVPELGLVVLKPGRESMPVFHNTRVLVEPEPKSMRN
                     LSSGVVPAVRQPLAEDKSLLPFFSDERVIRAAGGAGALSDWLLRHVKSCQWPHGDYHH
                     SETVIHRYGTGAMVLCWHCDNQLRDQTSESLGQLAHQNLSAWMIDVIRHAMNGSQERE
                     LSLAELSWWAVRNQVADALPEAVLRRSLGLRAEKIRSMYRESDIVPGEQTATSILKQR
                     TKNLAPLPHAHQQQNPPQEKTVVSIAVDPESPAQYLQRQKPQREEMPVYTRWVKTQKC
                     MTCGNQADDPHHIIGHGLGGMGTKADDLFVIPLCRKCHNELHAGVKDFEEKHGSQLLL
                     LIRFLMHARNSGVLKWKA"
     gene            complement(3246..3524)
                     /locus_tag="NBI74_003777"
     CDS             complement(3246..3524)
                     /locus_tag="NBI74_003777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1934052.1"
                     /translation="MARNAKYYNHDNSTVLAHTHERYSHAFKSDWYQHPPCTEEQAEW
                     LIQCYRRHGYEIKKALSLDYRHWIISVRLPYSERPPRPSRTFQQRIWR"
     gene            complement(3591..3842)
                     /locus_tag="NBI74_003778"
     CDS             complement(3591..3842)
                     /locus_tag="NBI74_003778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1934053.1"
                     /translation="MMNIEELRKIFCEDGLYAVCVENGNIVSHYRIVCLQKNGAALIN
                     FVDARVTDGFILHDGEFVTSLQALKEIGIKAGFSAFSEE"
     gene            complement(4059..4214)
                     /gene="hokD"
                     /locus_tag="NBI74_003779"
     CDS             complement(4059..4214)
                     /gene="hokD"
                     /locus_tag="NBI74_003779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HCD1934054.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     TAYEPEE"
     gene            complement(4773..5759)
                     /locus_tag="NBI74_003780"
     CDS             complement(4773..5759)
                     /locus_tag="NBI74_003780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000208091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1934055.1"
                     /translation="MTTITNKKQYPSEQYLNELITNIEFAARAPVEVVSAMAAELQKR
                     READSAEPVAYIFKHPAGKLFWALTDESNKEQADVIPVYAAPPVPVVSEEKPMPNPLK
                     MYAVDDVAAIAEVRGWNAYRAAMLQSQGEGNPHVTPARLPGGFTIEDAKVLHEDLVRS
                     HISQALSGEKMKKKDRDADLRWIHGVIVQAAWFVKASLEQNALSGNSPVIPGEVLSAI
                     REVARIRADFDDFDGDRRGIGDCLDEAEQELIVTINKYASQLAVEPVVPDDVLEQTVA
                     SPALGNQVSELTMWVKRLVSQLKKAQPDCKLPEKAMDYLKRNGLISVEDVLR"
     gene            complement(5756..6121)
                     /locus_tag="NBI74_003781"
     CDS             complement(5756..6121)
                     /locus_tag="NBI74_003781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HCD1934056.1"
                     /translation="MALTKKQRAELRMKFGGRCAYCGCELGEKWHADHVKPVIRFNGN
                     MLHQERDDISNMVPACHPCNLHKHCSSLEDYRRIISDGRREFLASGQGKALVRMGLVE
                     MKSDPVVFWFEKYQEGATA"
     gene            complement(6123..6530)
                     /locus_tag="NBI74_003782"
     CDS             complement(6123..6530)
                     /locus_tag="NBI74_003782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1934057.1"
                     /translation="MTELTKEQLIEEAKLKIAIAKCHTNSGMARVEGELFKIALASLE
                     AEPIAWKCGENIILFNPDTVEAYAKRAEISPKPLFAAPPVPVVSEELVKAVRFYEKVK
                     RENPPVETGAWKDAVDWVLKEACQVVNTGIKGD"
     gene            complement(6626..6982)
                     /locus_tag="NBI74_003783"
     CDS             complement(6626..6982)
                     /locus_tag="NBI74_003783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001514295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1934058.1"
                     /translation="MEIKPEDELSNIVLFPVKEDDPRNQVNFLYESSERPYCHHASVR
                     VDEKERQVRCKICGAVVEPFDWMLSVAKRETRLADDVRLLRQEEQERRKNIEKLIQIE
                     RNAKARICRATKSRTE"
     gene            complement(6960..7421)
                     /locus_tag="NBI74_003784"
     CDS             complement(6960..7421)
                     /locus_tag="NBI74_003784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001209482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
                     /protein_id="HCD1934059.1"
                     /translation="MREVNYEALREAAQNYQSTLAWYQAIPDSPNAERDCDAALAAFK
                     RHIRHREADIIADLLDGLEEAKSQLNEQREYYEGVISDGSKRIAELEAREVQLPTRYD
                     LRYGHPINADERHVMIPKENGSWLYLIDLEHALRVAGIRIKGEEHGNKTRG"
     gene            complement(7418..7714)
                     /locus_tag="NBI74_003785"
     CDS             complement(7418..7714)
                     /locus_tag="NBI74_003785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001266128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4406 domain-containing protein"
                     /protein_id="HCD1934060.1"
                     /translation="MRVRVYIAGPMTGYENFNREAFHKAEEELKREGHTVLNPAVLPD
                     GLTQPHYMDICMAMIRCVDAIYMLNGWQRSAGAKAELALAEKLGHAVIYQEVAQ"
     gene            complement(7711..8103)
                     /locus_tag="NBI74_003786"
     CDS             complement(7711..8103)
                     /locus_tag="NBI74_003786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001141097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HCD1934061.1"
                     /translation="MAKVFTPEEREEVKARIVEFVRLSGRETFRQLADKTGVSKTAIR
                     RLSGALAASGDVWLSGCGVFPSEQAYRVWRKTPEKAADPTLIRKLPDGEIRRYNRRQN
                     IICRECRQSEVMQRVLAFYRGNFQEVME"
     gene            complement(8119..8889)
                     /locus_tag="NBI74_003787"
     CDS             complement(8119..8889)
                     /locus_tag="NBI74_003787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1627 domain-containing protein"
                     /protein_id="HCD1934062.1"
                     /translation="METVFDALKAMGKATSVELAARLDISREEVLNELWELKRNGVVD
                     KTGHTWFLAGEGESRVTEERPVKSEAQDMLTGEVEQKVTADMMIEFIGQDGAKTCEEL
                     AGKFGVSTRKVASTLAVVTATGRLARVNQNGKFRYCMPGDNLPAEPKAALVTESDGKA
                     FPQPAGAALPVREAATQEEIKTETVADIVQPLPSFTETQADELIFPSLRRANLALRRA
                     KSDVQKWERVCAALRELNKHRDIVRQISDSSRRVVSEK"
     gene            complement(8923..9465)
                     /locus_tag="NBI74_003788"
     CDS             complement(8923..9465)
                     /locus_tag="NBI74_003788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001307798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HCD1934063.1"
                     /translation="MGEVYSNRWTQKNGAAPSKLWIAQIGAMTEQQIRQVCRQCMDRC
                     RAGETWPPDLAEFVALISESGANPFGLTVDAVMEEYRRWRDESWRYDGSDKYPWPQPV
                     LYHICLEMRTRGIERQMTQGELKRLAERQLTKWAKHVGNGMSVPPVRRQLEGAKHPQG
                     PTPIERLKQEYERRKAAGFI"
     gene            complement(9377..10417)
                     /locus_tag="NBI74_003789"
     CDS             complement(9377..10417)
                     /locus_tag="NBI74_003789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HCD1934064.1"
                     /translation="MSIDALRWAKKVKTGSSSSKSVLTWLADMCGADLCAYPSVSALA
                     EVTELNKKTVQDSLRHLMEIGLIVDTGERKGRTKQIVVYRLIGVEESVAEPEYTQKRE
                     SLKVGKIGAVNKNSTENGYVSAQNRPKNGTLSCMENNQRHPNFPSKTPKNGSRNPKEP
                     KDLNPTHNARESAPTSEQEVLSLQAAPLVFLDGLSEPIGKFPMTDSWYPSRDFRRRAA
                     LWGMALPETEFTPAELAAFRDYWAAEGKVFTQIQWEQKFARHVNHVRAQVKPVSKGVN
                     HAAAPGGTASRAVQEIRAAREQWERENGFISDGNGLEAVGTHGGGLFEPLDPEERGRT
                     FEALDCTDWRDD"
     gene            complement(10389..>10600)
                     /locus_tag="NBI74_003790"
     CDS             complement(10389..>10600)
                     /locus_tag="NBI74_003790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1934065.1"
                     /translation="IAGLNKEYGDVVESGLLFADPAVVERETDELIEKAIAFKHAYRQ
                     QYQYYFADKQMSARGLYEYRCTTMG"
BASE COUNT         2494 a         2944 c         2258 g         2904 t
ORIGIN      
        1 gcataaacaa ggaaactaaa tgaagtggat tgtgattgat acagttatcc agccatcatg
       61 cggaatatct ttttcagtca tatggagtaa aataaaatta ataatctggt atcaatcgga
      121 tgctttctta cctcctgaaa gtatatttac actgactcac acaggtatca tgctcaataa
      181 caaagtgcta cctgtaacca tttacaacgt agtaccattc aataaaacat tctggaattt
      241 aatcaaaaac agccaggaat gccctacaaa tacagataac gtattgaatg aatgctttaa
      301 taaccgttgc actctgcaaa tatgtcctta tgggctaaaa caacaaagtc cataaggagt
      361 ttactcacat ctgacaaaat caatataaac agcccctccg gagaggggct ggagagtggc
      421 gctatgtgcc attgcatggt gccgggtgcc tcccggtgaa ttcagtacca gcacctgaat
      481 ccgcgattat cccatatacc tactcgctga ttgcccctcc gcacaggggg attcaccatg
      541 ccagtttctt ttaacaaact ccccgcaaac cagacaacag tcaaccgcct gaattgtgaa
      601 gtatttaaaa atttctcccg ctaactgata cccggctaac agtctggcgt tttctttttc
      661 agcaacggga aagcaacaac caccacaccc accagccgcc catttaccac aaataaaaaa
      721 gccttcagga ctgaaggtgt ctgtaacaac caaactgata gtctgccaga cccgccataa
      781 caagctgggt cagtattaac tggcagcgtt cgcgtgaaag gtaagtattc tgtgcaatct
      841 ccctgactgt cgccgggtcg gtaacgctta attcattaaa caccactctg gcggcttctg
      901 tcatatcctg ctgttttagc atgtcttttt cccttttccg gttaacgtga cataccaata
      961 actcttgtcg aaaaagccgg caagctgaaa gacccgtatt cgcaaccacc agcgcgttta
     1021 ctgtactgac gcgattttcg gacataaaaa aaaccacctg gcggtggttt tttcttactt
     1081 tgccatcacg tacaaaatcg gcaaaatatc agatttatac gaaacatacg cgatttaatt
     1141 gacttttgca atatctcgtc gtgaaaaggt cgctttttgt tgcgctctta ttttcacgga
     1201 gcaaatcaag gattctctat cgaggcgctt aaaaatatcg cacatctcac gccagtagtt
     1261 cgcataatta tggctccagt tatcaggctt aactccacac agtctggcaa gctcctgtct
     1321 ctggtagacc tcacacccgg taacccatcc tctgacatcc tgtgccgcca gccagatcaa
     1381 cttcttcaca cgctccagcg ttttcactgc aatttttctg gagccggact gagttttaaa
     1441 ttcactccac ccccactgcg ttatcgcgat ctgatgctcc cagcaaatgt ttccgccata
     1501 acaccacaac aaccacgcct tctgatgttc ttccagttcc agaacagcac gccgccacga
     1561 tgatgttgca aactcaacag gactgaccag cgcaattgat gagcctttcg ccagtgattg
     1621 tttaccctgg atcgggggat tatcccgcgt gatcattttt ccggttacct catcgcggta
     1681 acgaattttt ttgcgtctgt aacgccctgt atcgaacagg gcattttcct gccaggcttc
     1741 cagctggcat tttgtcgacc cactgagatc tgcagtggca atcatgagtt gctcacgaac
     1801 aaactgtaaa tactggttat tcatgcacac ccacctctgt aattcttatc tccagccgtc
     1861 caccagatac tggctggcca cgtacaatat tgatttcatc aaactgctca tcgtccatta
     1921 acaaccccgc gtgcgtcagc gcatccagtg gtgctttcag aatattgtcc aggtcacgac
     1981 gacgcttatc cggtggctct gcaataattt ttattgccag ccgtccggac aggcttaatt
     2041 tcagtcgctg ctggcgaaca ataagcgcca ctgcccggcg ataacgctcc ccggcttttg
     2101 atacaaaata tgtgctgcca cggcgtcgcc agtaagtgtt caccgtcggc gagtaaggca
     2161 aaacaaattc tatgcgttcg gtcatttatg ctttccactt cagaacaccc gaatttctcg
     2221 cgtgcattaa aaaacgaatc agcaacaaca actgactacc gtgtttttct tcaaaatctt
     2281 ttaccccggc gtgtagttcg ttatggcatt tacgacacag cggaataaca aacaaatcgt
     2341 cagcctttgt tcccatccct cccagtccat gaccaatgat gtgatgcgga tcatctgcct
     2401 gattgccaca cgtcatgcat ttctgcgttt ttacccagcg cgtgtataca ggcatctctt
     2461 cccgttgtgg tttctggcgc tggagatact gagccggaga ttccggatca acggcaatgc
     2521 tgaccaccgt cttttcctgt ggtgggttct gttgctggtg ggcgtgaggc agcggcgcaa
     2581 gattttttgt gcgctgtttc agtatgctgg tggcggtctg ctctcccggt acgatgtcgc
     2641 tttcacggta cattgagcgg attttttccg cacgcaaccc cagcgaacga cgtaataccg
     2701 cttccggtag cgcgtccgcc acctgattgc ggaccgccca ccaggataat tcagccagcg
     2761 ataattcccg ttcctgcgag ccattcattg catggcgtat gacgtcaatc atccatgcag
     2821 acaggttttg gtgagcaagt tgcccgagtg attcggaggt ctggtcgcgc agctggttgt
     2881 cgcagtgcca gcacaacacc attgcgccgg taccataacg gtgaatgacg gtttcactgt
     2941 ggtgataatc gccgtgtggc cactggcagg atttaacatg gcgcagtaac cagtcagaca
     3001 atgcgccagc gccaccagca gcacgaatca ctcgttcgtc gctgaaaaat ggcagtaatg
     3061 atttatcctc cgccagcggc tggcgaacgg caggaacgac cccggacgac agattacgca
     3121 tgcttttcgg ttccggctcc accagtaccc gggtattgtg gaataccggc atggattcac
     3181 ggcccggttt tagcaccacc agcccaagtt ccggtaccag aacaggtcga agtaataccc
     3241 gcacgttacc tccagatgcg ttgctggaat gtgcgggacg gacgcggtgg ccgttcggag
     3301 taagggagcc tgacggagat tatccagtga cgataatcga ggctgagggc tttcttaatc
     3361 tcgtatccgt gtctgcggta gcactgaatt agccactcgg cctgttcttc agtgcatggg
     3421 ggatgctggt accagtctga cttaaatgca tgagaatacc gctcgtgcgt gtgggcaaga
     3481 acggtcgaat tatcatgatt gtaatatttt gcgttgcgtg ccatcggttt tctccggtgg
     3541 cacggtgtta ctcagcggga gttcagcccc gcgcaagatt gtagatgagt ttattcttct
     3601 gaaaaagcag aaaagccagc ttttattccg atctctttca atgcctgtaa tgaagtgaca
     3661 aactcaccgt cgtgcaagat aaatccgtcc gtcactcggg catccacaaa attaattaac
     3721 gcagccccat ttttttgcaa acacacaatg cggtaatggc taacaatatt tccattttca
     3781 acgcacacag catagaggcc atcttcacaa aaaattttac gcagttcttc gatgttcatc
     3841 atcagaatcc ttccggataa ttagctctcc cctttaaggg accatccctc ttatccctgc
     3901 gcgctactta agtatttttg attctattcc ggcaccgtcc agaacttcaa atgcgttgaa
     3961 aataaaaaca aaaacccgcc gaagcgggtt aagtgcgggt gcgttgagga tgcctgccac
     4021 atcagaggtg gcgagggatt tctccctcgc ctggtctctt actcctcagg ttcgtaagct
     4081 gtgaagacag cgacctccgt ctggccggtt cggattcgta cctcgcagag gtctttcctc
     4141 gttaccagtg ccgtcactat gacggttaaa cagatgacga tcagggcgat taacatcgcc
     4201 ttttgctgct tcatagcctg cttctccttg acctttcggt ccgtaagagg ctaatctcta
     4261 tgtgtcgcat agatatggcc tcagattaat gttaagcgtc ttgcaggacg cgtaatgtta
     4321 actggggctt ttctctatct gccttttggt gttcatgcct gagacagata gcctcaagca
     4381 cccgcagtta ttctacttaa ctaagatttc cccgcaaacc gtttttgtcc ggcacagtaa
     4441 atatccaact aaaccaatgg cgttcgctgt atttaccgcc agtattcaat gcacatgacc
     4501 gccatgaaca cccctaaaaa aagggcattt atatgtccaa acattaatat caaaacatca
     4561 attttttcca tataccttgc tgtgaagatg atgggcatac atgatgcgaa caaccagaac
     4621 gcaacaaaca aaaactgcaa tgcgtttttc attattcccc ctacaatcaa tgtgcaataa
     4681 catttaaaca cacctcaatt tggccggaca tataaatatc taaaccagaa aaaatcactt
     4741 acatagcgtt acaaactctt tagtctaaat attcatcgta aaacatcctc cacgcttatc
     4801 agtccatttc gtttcaggta atccatcgcc ttctccggta atttgcagtc cggctgagct
     4861 tttttcagtt gactgaccag tcgtttaacc cacattgtta attcgctaac ctggttacct
     4921 agcgctggcg acgctactgt ctgttccaga acgtcatcag gtactaccgg ctctactgcc
     4981 aactgactgg catatttgtt aatggtaacg ataagctctt gctcggcctc atccagacaa
     5041 tcaccgatac ctcgcctgtc accgtcaaaa tcatcgaaat cggcgcgaat cctggcaacc
     5101 tcccggattg cggacaacac ctcaccagga attaccggag agttgcccga tagtgcattc
     5161 tgctccagtg atgcctttac aaaccacgcg gcctgaacta ttacgccatg aatccagcgc
     5221 aaatcagcat cgcgatcttt ctttttcatc ttttcgccac ttaaagcctg gcttatgtgg
     5281 ctacgtacaa ggtcttcatg taacaccttc gcatcctcaa tggtgaaacc accaggcagg
     5341 cgagccgggg ttacatgagg gttgccttcc ccctgactct gaagcatggc ggcgcggtag
     5401 gcattccagc ctctcacctc tgcaatagcg gcaacatcat cgaccgcgta cattttaaga
     5461 gggttaggca ttggtttttc ttcagatact actggcactg gaggggcggc ataaacagga
     5521 ataacgtccg cttgctcttt attgctttca tccgttaaag cccagaataa tttcccggcc
     5581 ggatgtttga aaatataagc aactggttct gcgctatcag cttcgcgccg cttctgtagc
     5641 tctgctgcca ttgcgctcac gacttcaact ggtgcccttg cagcaaactc tatgttggtg
     5701 ataagctcat taagatattg ctcgctggga tactgtttct tattggtaat agtggtcatg
     5761 ctgtagcccc ttcttgatat ttttcaaacc agaacacaac cgggtcagat ttcatttcaa
     5821 ccaatcccat acgaaccagc gctttgcctt gcccggacgc aaggaattca cgacgaccat
     5881 cactgataat tcgccgatag tcttccaggc tactgcaatg cttgtgcaga ttgcatgggt
     5941 ggcatgccgg aaccatgttg gatatatcgt cacgttcctg gtgaagcata tttccattaa
     6001 aacgaatgac cggttttaca tggtctgcat gccacttttc gccaagttca cagccgcaat
     6061 aagcacagcg accgccaaac ttcatgcgca attctgcacg ttgttttttc gtcagtgcca
     6121 tatcagtctc ctttgatgcc agtgtttaca acctggcagg cctctttgag cacccagtca
     6181 acagcgtctt tccatgctcc ggtttcgact ggcggatttt cacgcttaac cttttcatag
     6241 aagcgcacgg ctttaaccag ttcttcagac acaaccggca caggtggagc tgcgaatagt
     6301 ggtttaggtg atatctccgc acgttttgcg tatgcttcaa ctgtgtcagg attaaacagg
     6361 attatgtttt cgccgcattt ccacgctatc ggttccgctt ctagcgatgc cagtgcaatt
     6421 ttgaataact caccctctac ccgtgccatc cctgaattcg tgtggcattt cgcaatcgct
     6481 atttttaatt tggcttcttc gattaattgc tcttttgtta attcagtcat ttttcattac
     6541 cgccctttcg ggcggcctcc tgatgttctg agggtgcaga aatccctccg gttaaggata
     6601 aaatttttaa cagtgctaaa tttaattatt cagttctgga ttttgtcgcc ctgcatatcc
     6661 gcgctttcgc gttacgctca atctgaatta acttttctat atttttccgc ctttcctgtt
     6721 cctcctggcg caatagcctt acatcatctg ccagtctggt ttctcttttc gccacagaga
     6781 gcatccagtc aaacggctcc acaactgcac cgcagatttt acagcggacc tgacgctctt
     6841 tttcgtcaac ccgaacagag gcgtgatgac aatatggtct ttccgatgac tcataaagaa
     6901 aattaacctg attacgaggg tcatcctctt ttaccggaaa taaaacgata ttgcttaact
     6961 catcctctgg ttttatttcc atgctcctct cctttgatgc gaatgccagc gacgcgtaat
     7021 gcgtgttcta ggtcaatcag gtaaagccaa ctgccatttt ctttaggtat catgacatgt
     7081 cgctcatctg catttatcgg atgtccatat cgaaggtcgt agcgagtcgg taattgaact
     7141 tcccgcgctt ccagttcagc aatacgctta ctcccatcag agataacgcc ttcgtaatac
     7201 tcacgctgct cgttgagttg tgattttgct tcttccagtc catccagcaa atcagcgata
     7261 atatccgctt cccgatgacg gatgtgacgc ttaaacgcag caagagccgc atcacaatcc
     7321 cgttcagcat ttgggctgtc cgggatagcc tgataccacg ccagcgtcga ctgatagttt
     7381 tgtgctgcct cacgaagcgc ctcatagtta acctctctca ttgagccacc tcctgataaa
     7441 tcaccgcatg ccccagtttc tccgccagtg ccagctctgc cttagcgccc gctgaccgct
     7501 gccagccatt cagcatgtaa atcgcatcca cacaacgaat cattgccatg caaatatcca
     7561 tgtagtgcgg ctgtgtcagc ccgtccggaa gtactgccgg gtttaagacg gtatgccctt
     7621 cccgtttcag ttcctcttcc gccttgtgaa acgcctcacg gttgaaattt tcatatcccg
     7681 tcattggacc ggcaatataa actctcaccc tcactccatc acctcctgaa agtttccccg
     7741 atagaacgcc agcacacgct gcatgacttc gctctggcgg cactcacgac aaattatgtt
     7801 ctgccgtctg ttgtaacgac gtatttctcc gtcaggtaac tttcgaatca gtgtcgggtc
     7861 agcagccttc tccggtgtct tacgccatac gcgatacgcc tgctctgatg gaaatacccc
     7921 gcaaccagag agccagacat caccactggc cgcaagcgca ccagataaac gacgaatagc
     7981 ggtcttactg acacccgttt tatctgccag ttgtcgaaaa gtttctcgtc cgctcaggcg
     8041 cacgaattcc acaatgcgcg ccttcacttc ttcccgctct tctggtgtaa atacttttgc
     8101 cataagcgcc tccggcaatc acttttccga tacaacacgg cgggaagaat cactaatctg
     8161 tcgaacaata tcccggtgct tgttcagctc ccgcagcgcg gcgcagactc gctcccactt
     8221 ctgaacatca cttttcgccc tgcgcagcgc caggtttgcc ctgcgaaggg acggaaaaat
     8281 cagctcatct gcttgcgttt cggtaaacga tggcaacggc tgcacaatgt ccgccacagt
     8341 ttctgtttta atttcttcct gtgttgcggc ttcccggact ggtaacgcag cacctgctgg
     8401 ctgaggaaag gccttaccat cactttccgt taccagcgcg gctttcggct ctgctggtaa
     8461 attatcgccc ggcatgcagt aacgaaattt accgttctga ttaacgcgtg ccagccgccc
     8521 cgttgcggtt accaccgcca gcgtggaggc aaccttgcga gtactgacgc cgaacttacc
     8581 cgccagttcc tcacacgttt tagcaccatc ctgaccgata aactcaatca tcatgtctgc
     8641 ggtaactttt tgttcgacct ccccggtcag catatcctgt gcttcagatt ttactggccg
     8701 ctcttcggtt acccgggatt caccttcgcc agccagaaac caggtgtgac cagttttatc
     8761 aacgacgcca tttcttttga gttcccacag ctcgttgaga acctcttcac gactgatatc
     8821 aagtcgcgcg gccagttcta ccgatgtggc ttttcccatt gctttcagtg cgtcaaatac
     8881 ggtttccatt aaaatttcct ccgacaaaat cgtttctcag attcaaataa aaccagctgc
     8941 cttccggcgt tcgtattcct gtttcagccg ttcaattggc gttggccctt gcgggtgttt
     9001 cgccccttcc agttgtcgtc gcactggcgg aacactcatc ccgttaccaa catgctttgc
     9061 ccatttcgtc agttgccgtt ctgcaagtcg ttttaactca ccctgcgtca tctggcgctc
     9121 aatccctctg gtacgcattt cgaggcagat gtggtacagc acaggctgag gccacggata
     9181 tttgtcgctt ccgtcatatc gccaggactc atcacgccag cggcggtact cctccatcac
     9241 agcatccacc gtcaggccaa atggattggc cccgctttct gaaatcagcg ccacaaactc
     9301 agccaggtcc ggaggccatg tttcacccgc ccggcagcgg tccatgcact ggcggcagac
     9361 ctgtcggatt tgctgctcag tcatcgcgcc aatctgtgca atccagagct tcgaaggtgc
     9421 ggccccgttc ttctgggtcc agcggttcga ataaacctcc cccatgagtt cccacagctt
     9481 ccagaccgtt tccgtcgctg ataaatccgt tttcacgttc ccactgctca cgtgctgccc
     9541 gaatttcctg aactgcccgt gatgcggtgc cacctggtgc tgctgcatgg tttaccccct
     9601 tgctgactgg tttaacctgc gccctgacgt gatttacgtg acgggcgaat ttctgctccc
     9661 actgaatctg cgtaaacact ttcccctccg ctgcccagta gtcccggaag gcggcaagtt
     9721 cagcaggtgt aaattctgtc tccggcaaag ccatccccca caacgcagcc cgtcgtcgaa
     9781 aatcccgtga cggataccag ctatcggtca tcggaaattt tccgatgggt tcgctcaggc
     9841 catccaggaa tacaaggggt gctgcctgta acgacaaaac ttcctgctca ctggtcggag
     9901 cactctcgcg tgcgttatgt gtggggttta gatctttggg ttcctttggg ttccgtgatc
     9961 cgtttttggg tgtctttgat ggaaaatttg ggtgtctttg gttattttcc atgcagctaa
    10021 gagttccgtt tttgggtctg ttttgtgctg aaacataacc attttcggta ctgtttttat
    10081 taacagcacc aattttaccc acctttaaag actcccgttt ttgggtgtat tcaggctcgg
    10141 caacactttc ttctacaccg ataagtcggt acaccacaat ttgctttgtt ctgccttttc
    10201 tctcaccggt atcaacaatt aacccaatct ccatcaggtg tcgtaagctg tcctgcacag
    10261 tctttttgtt cagttccgtt acttctgcca gtgcagatac agacgggtat gcacacaaat
    10321 cggcaccgca catatcagca agccaggtca atacagactt actggatgaa ctgccggttt
    10381 tcaccttttt agcccatcgt agtgcatcga tactcataca aacccctggc agacatttgt
    10441 ttatctgcaa agtaatattg atattgctga cgatacgcat gcttgaaagc aatagctttt
    10501 tctataagct cgtcagtctc acgttccaca acagctggat ccgcaaaaag cagcccggac
    10561 tccaccacat cgccatattc tttgtttaat ccggcgatca
//
LOCUS       DAHHTG010000139        10549 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.140, whole genome shotgun sequence.
ACCESSION   DAHHTG010000139 DAHHTG010000000
VERSION     DAHHTG010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10549)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10549)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10549
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.140"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            80..1054
                     /gene="cysB"
                     /locus_tag="NBI74_003791"
     CDS             80..1054
                     /gene="cysB"
                     /locus_tag="NBI74_003791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012017585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator CysB"
                     /protein_id="HCD1934066.1"
                     /translation="MKLQQLRYIVEVVNHNLNVSSTAEGLYTSQPGISKQVRMLEDEL
                     GIQIFSRSGKHLTQVTPAGQEIIRIAREVLSKVDAIKSVAGEHTWPDKGSLYIATTHT
                     QARYALPNVIKGFIERYPRVSLHMHQGSPTQIADAVSKGNADFAIATEALHLYEDLVM
                     LPCYHWNRAIVVTPDHPLAGKKAITIEELAQYPLVTYTFGFTGRSELDTAFNRAGLTP
                     RIVFTATDADVIKTYVRLGLGVGVIASMAVDPVADPDLVRVDAHDIFSHSTTKIGFRR
                     STFLRSYMYDFIQRFAPHLTRDVVDAAVALRSNEEIEVMFKDIKLPEK"
     gene            1348..1512
                     /locus_tag="NBI74_003792"
     CDS             1348..1512
                     /locus_tag="NBI74_003792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmiA family putative membrane protein"
                     /protein_id="HCD1934067.1"
                     /translation="MLISDGDYMRLAMPSGNQEPRRNPELKRKAWLAVFLGSALFWVV
                     VALLIWKLWG"
     gene            1515..1682
                     /locus_tag="NBI74_003793"
     CDS             1515..1682
                     /locus_tag="NBI74_003793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_304581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1934068.1"
                     /translation="MVGQEQLESSPLCQHSDNEPEAKRECSVVIPDDWQLTSQQQAFI
                     ELFAEDDQPKQ"
     gene            1796..1891
                     /locus_tag="NBI74_003794"
     CDS             1796..1891
                     /locus_tag="NBI74_003794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1934069.1"
                     /translation="MINTNMKYWSWMGAFSLSMLFWAELLWIITH"
     gene            2041..4716
                     /gene="acnA"
                     /locus_tag="NBI74_003795"
     CDS             2041..4716
                     /gene="acnA"
                     /locus_tag="NBI74_003795"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HCD1934070.1"
                     /translation="MSSTLREASKDTLQAKDKTYHYYSLPLAAKSLGDITRLPKSLKV
                     LLENLLRWQDGKSVTEEDIHALAGWLKNAHADREIAYRPARVLMQDFTGVPAVVDLAA
                     MREAVKRLGGDTAKVNPLSPVDLVIDHSVTVDRFGDDEAFEENVRLEMERNHERYVFL
                     KWGKQAFSRFSVVPPGTGICHQVNLEYLGKAVWSELQDGEWIAYPDTLVGTDSHTTMI
                     NGLGVLGWGVGGIEAEAAMLGQPVSMLIPDVVGFKLTGKLREGITATDLVLTVTQMLR
                     KHGVVGKFVEFYGDGLDSLPLADRATIANMSPEYGATCGFFPIDAVTLDYMRLSGRSE
                     DQVELVEKYAKAQGMWRNPGDEPIFTSVLELDMNDVEASLAGPKRPQDRVALPDVPKA
                     FAASSELEVNATHKDRLPVDYVMNGHQYQLPDGAVVIAAITSCTNTSNPSVLMAAGLL
                     AKKAVTLGLKRQPWVKASLAPGSKVVSDYLAKAKLTPYLDELGFNLVGYGCTTCIGNS
                     GPLPDPIETAIKKGDLTVGAVLSGNRNFEGRIHPLVKTNWLASPPLVVAYALAGNMNI
                     NLASEPIGHDRKGEPVFLKDIWPSAQEIARAVDQVSTEMFRKEYAEVFEGTAEWKEIN
                     VTRSDTYGWQEDSTYIRLSPFFDEMQATPAPVEDIHGARILAMLGDSVTTDHISPAGS
                     IKPDSPAGRYLQGRGVERKDFNSYGSRRGNHEVMMRGTFANIRIRNEMVPGVEGGMTR
                     HLPDSDVVSIYDAAMRYKQEQTPLAVIAGKEYGSGSSRDWAAKGPRLLGIRVVIAESF
                     ERIHRSNLIGMGILPLEFPQGVTRKTLELTGEEKIDIGDLQNLQPGATVPVMLTRADG
                     SQEVVPCHCRIDTATELTYYQNDGILHYVIRNMLK"
     gene            complement(4780..5370)
                     /gene="ribA"
                     /locus_tag="NBI74_003796"
     CDS             complement(4780..5370)
                     /gene="ribA"
                     /locus_tag="NBI74_003796"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HCD1934071.1"
                     /translation="MQLKRVAEAKLPTPWGDFLMVGFEELATGHDHVALVYGDISGHT
                     PVLARVHSECLTGDALFSLRCDCGFQLEAALTQIAEEGRGILLYHRQEGRNIGLLNKI
                     RAYALQDQGYDTVEANHQLGFAADERDFTLCADMFKLLGVNEVRLLTNNPKKVEILTE
                     AGINIIERVPLIVGRNPNNEHYLDTKAEKMGHLLNK"
     gene            5540..6304
                     /gene="pgpB"
                     /locus_tag="NBI74_003797"
     CDS             5540..6304
                     /gene="pgpB"
                     /locus_tag="NBI74_003797"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase B"
                     /protein_id="HCD1934072.1"
                     /translation="MRSIARRTAVGAALLLVMPVAVWISGWCWQPGEQSWLLKAAFWV
                     TETVTQPWGVITHLILFGWFLWCLRFRIKAAIMLFAILAAAILMGQGVKSWIKDKVQE
                     PRPFVIWLEKTHHIPVDEFYTLKRAERGNLVKEQLAEEKNIPQYLRSHWQKETGFAFP
                     SGHTMFAASWALLAVGLLWPRRRTLTIAILLVWATGVMGSRLLLGMHWPRDLVVATLI
                     SWALVAVATWLAQRICGPLTPPAEENREIAQREQES"
     gene            6453..6761
                     /gene="lapA"
                     /locus_tag="NBI74_003798"
     CDS             6453..6761
                     /gene="lapA"
                     /locus_tag="NBI74_003798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA"
                     /protein_id="HCD1934073.1"
                     /translation="MKYLLIFLLVLAIFVISVTLGAQNDQQVTFNYLLAQGEYRISTL
                     LAVLFAAGFAIGWLICGLFWLRVRVSLARAERKIKRLENQLSPATDVAVAPHSSAAKE
                     "
     gene            6768..7937
                     /gene="lapB"
                     /locus_tag="NBI74_003799"
     CDS             6768..7937
                     /gene="lapB"
                     /locus_tag="NBI74_003799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapB"
                     /protein_id="HCD1934074.1"
                     /translation="MLELLFLLLPVAAAYGWYMGRRSAQQNKQDEANRLSRDYVAGVN
                     FLLSNQQDKAVDLFLDMLKEDTGTVEAHLTLGNLFRSRGEVDRAIRIHQTLMESASLT
                     YEQRLLAIQQLGRDYMAAGLYDRAEDMFNQLTDETDFRIGALQQLLQIYQATSEWQKA
                     IDVAERLVKLGKDKQRVEIAHFYCELALQHMASDDLDRAMTLLKKGAAADKNSARVSI
                     MMGRVFMAKAEYAKAVESLQRVISQDRELVSETLEMLQTCYQQLGKTAEWAEFLQRAV
                     EENTGADAELMLADIIEARDGSEAAQVYITRQLQRHPTMRVFHKLMDYHLNEAEEGRA
                     KESLMVLRDMVGEKVRSKPRYRCQKCGFTAYTLYWHCPSCRAWSTIKPIRGLDGL"
     gene            8129..8866
                     /gene="pyrF"
                     /locus_tag="NBI74_003800"
     CDS             8129..8866
                     /gene="pyrF"
                     /locus_tag="NBI74_003800"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HCD1934075.1"
                     /translation="MTLTASSSSRAVTNSPVVVALDYHNRDAAMAFVDKIDPRDCRLK
                     VGKEMFTLFGPQFVSELQQRGFDIFLDLKFHDIPNTAAHAVAAAADLGVWMVNVHASG
                     GARMMAAAREALVPFGKDAPLLIAVTVLTSMEACDLADLGVTLSPADYAERLAALTQK
                     CGLDGVVCSAQEAVRFKQVFGQEFKLVTPGIRPQGSDAGDQRRIMTPEQALAAGVDYM
                     VIGRPVTQSVDPAQTLKAINASLQRSA"
     gene            8866..9192
                     /gene="yciH"
                     /locus_tag="NBI74_003801"
     CDS             8866..9192
                     /gene="yciH"
                     /locus_tag="NBI74_003801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response translation initiation inhibitor
                     YciH"
                     /protein_id="HCD1934076.1"
                     /translation="MSDSNSRLVYSTETGRIDEPKAAPVRPKGDGVVRIQRQTSGRKG
                     KGVCLITGVDLDDAELTKLAAELKKKCGCGGAVKDGIIEIQGDKRDLLKSLLEAKGMK
                     VKLAGG"
     gene            complement(9318..9536)
                     /gene="osmB"
                     /locus_tag="NBI74_003802"
     CDS             complement(9318..9536)
                     /gene="osmB"
                     /locus_tag="NBI74_003802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmB"
                     /protein_id="HCD1934077.1"
                     /translation="MFVTSKKMTAAVLAITLAMSLSACSNWSKRDRNTAIGAGAGALG
                     GAVLTDGSTLGTLGGAAVGGVIGHQVGK"
     gene            complement(9805..>10549)
                     /locus_tag="NBI74_003803"
     CDS             complement(9805..>10549)
                     /locus_tag="NBI74_003803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCD1934078.1"
                     /translation="SRQQTILQMVIDQGQVSVTDLAKATGVSEVTIRQDLNTLEKLSY
                     LRRAHGFAVSLDSDDVETRMMSNYTLKRELAEFAASLVQPGETIFIENGSSNALLART
                     LGEQKKNVTIITVSSYIAHLLKDAPCEVILLGGVYQKKSESMVGPLTRQCIQQVHFSK
                     AFIGIDGWQPETGFTGRDMMRTDVVNAVLEKECEAIVLTDSSKFGAVHSYSIGPVERF
                     NRVITDSKIRASDLMHLEQSKLTVHVVDI"
BASE COUNT         2629 a         2464 c         2723 g         2733 t
ORIGIN      
        1 tattattaaa tcgtattagt cacccgcctg gtgtgacgaa aaaacgatgt tctgatggcg
       61 tctaagtgga tggtttaaca tgaaattaca acaacttcgc tatattgttg aggtggtcaa
      121 tcataacctg aatgtctcat caacggcgga aggactttac acatcacaac ccggaatcag
      181 taaacaagtc agaatgctgg aagacgagct gggcattcaa attttttccc gaagcggcaa
      241 gcacctgacg caggtaacgc cagcaggaca agaaataatt cgtatcgctc gtgaagttct
      301 gtcgaaagtc gatgccataa aatcggtcgc cggagagcac acctggccgg ataaaggctc
      361 gctgtatatc gccaccacgc atactcaggc acgctatgca ttaccaaacg ttatcaaagg
      421 ctttattgag cgttatcctc gcgtttcttt gcatatgcac cagggctcgc cgacacaaat
      481 tgctgatgcc gtctctaaag gcaatgccga tttcgctatc gccacggaag cgctgcatct
      541 gtatgaagat ttagtgatgc taccgtgcta ccactggaat cgggctatag tagtcactcc
      601 ggatcacccg ctggcaggta aaaaagccat taccattgaa gaactggcgc aatatccgtt
      661 ggtgacatat accttcggct ttaccggacg ttcagaactg gatactgcct ttaaccgcgc
      721 agggttaacg ccgcgtatcg tcttcacggc aacggatgct gacgtcatta aaacttacgt
      781 ccggttaggg ctgggagtag gggtcattgc cagcatggcg gtggatccgg tcgccgatcc
      841 cgaccttgtg cgtgttgatg ctcacgacat cttcagccac agtacaacca aaattggttt
      901 tcgccgtagt acttttttgc gtagttatat gtatgatttc attcagcgtt ttgcaccgca
      961 tttaacgcgt gatgtcgttg atgcggctgt cgcattgcgc tctaatgaag aaattgaggt
     1021 catgtttaaa gatataaaac tgccggaaaa ataatttatt gcgttatttt tggcaccttt
     1081 tatggagcga aggtgccgga atatattctc ctttgttact tatttgtatc cggtgcacaa
     1141 tatttttatc cctcatattt acagggtaaa ttacctgtaa agtagtgtct aattacccgc
     1201 ctcgcaaact ccagttttca aatatctatt tcaggtcaaa agattgaatt attcacggag
     1261 acgattcgta aattcgctgg atttttgact aaagtttctt taggatttgt ctcatcgtat
     1321 gattaattac accaatcgtt caacgtcttg ctgataagtg atggcgatta tatgaggtta
     1381 gcaatgcctt ctggaaatca ggaaccccgc cgcaatcctg aattaaaacg taaagcctgg
     1441 ctggcggttt ttcttggttc tgcacttttc tgggtggttg tcgcactgct tatttggaaa
     1501 ttgtggggat aactatggtc ggtcaggagc aactggagtc gtcaccatta tgtcagcata
     1561 gtgacaatga accagaagca aaacgggaat gttccgtcgt tattccagac gactggcaac
     1621 taacatcgca gcaacaagcc tttatagaac tgtttgctga agatgatcag ccgaaacaat
     1681 aattatcatc attcttatta cccattttta atgaattaca gggcttttaa tacaccgcag
     1741 caataacagg ttgagttatc tcaacacaaa gtaataaccg ttaagggtgt agcctatgat
     1801 caacacaaat atgaaatatt ggtcctggat gggcgcattt tctctgtcga tgctcttctg
     1861 ggccgaactc ctctggatca ttactcactg atccctgacc ccgctgtttg ctttgcctca
     1921 aggtttctcc tcttttatca atttgggttg ttatcaaatc gttacgcgat gtttgtgtta
     1981 tctttaatat tcaccctgaa gagaatcagg gcttcgcaac cctgtcatta aggaggagct
     2041 atgtcgtcaa ccctacgaga agccagtaag gacacgttgc aggccaaaga taaaacttac
     2101 cactactaca gcttgccgct tgctgccaaa tcactgggcg atatcacccg tctacccaag
     2161 tcactcaaag ttttgctcga aaacctgctg cgttggcagg atggtaaatc ggttaccgaa
     2221 gaggatatcc acgcgctggc gggatggctg aaaaatgccc atgctgaccg tgaaattgcc
     2281 taccgcccgg caagagtgct gatgcaggac tttaccggcg tacctgccgt tgttgatctg
     2341 gcggcaatgc gcgaagcggt taaacgcctc ggcggcgata ctgcaaaggt taacccactc
     2401 tcaccggtcg acctggtcat tgaccactcg gtgaccgtcg atcgttttgg tgatgatgag
     2461 gcatttgaag aaaacgtacg cctggaaatg gagcgcaacc acgaacgtta tgtgttcctg
     2521 aaatggggaa agcaggcgtt cagtcggttt agcgtcgtgc cgccaggcac aggcatttgc
     2581 catcaggtta acctcgaata tctcggcaaa gcagtgtgga gcgaattgca ggacggtgaa
     2641 tggattgctt atccggatac actcgttggt actgactcgc acaccaccat gatcaacggc
     2701 cttggcgtgc tggggtgggg cgttggtggc atcgaagcag aagccgcaat gttaggccag
     2761 ccggtttcca tgcttatccc ggacgtagtg ggctttaaac ttaccggaaa attacgtgaa
     2821 ggtattaccg ccacagacct ggttctcact gttacccaaa tgttgcgcaa acatggcgtg
     2881 gtggggaaat tcgtcgaatt ttatggtgat gggctggatt cactaccgct ggcggatcgc
     2941 gccaccattg ccaatatgtc gccagaatat ggtgccacct gtggcttctt cccaatcgat
     3001 gctgtcaccc tcgattacat gcgtttaagc gggcgcagtg aagatcaggt cgagttggtc
     3061 gaaaaatatg ccaaagcgca gggcatgtgg cgtaatccgg gcgatgaacc aatttttacc
     3121 agtgtgttag aactggatat gaatgacgtt gaagcgagcc tggcagggcc aaaacgcccg
     3181 caggatcgcg ttgcattgcc cgatgtacca aaagcatttg ctgccagtag cgaactggaa
     3241 gtgaatgcca cgcataaaga tcgcctgccg gtcgattatg ttatgaacgg acatcagtat
     3301 cagttacctg atggcgctgt ggtcattgct gcgataacgt cgtgcaccaa cacctctaac
     3361 ccaagtgtgc tgatggccgc aggcttgctg gcgaaaaaag ccgtaactct gggcctcaag
     3421 cggcaaccat gggtcaaagc gtcgctggca ccgggttcga aagtcgtttc tgattatctg
     3481 gcaaaagcga aactgacacc gtatctcgat gaactgggat ttaaccttgt gggatacggt
     3541 tgtaccacct gtattggtaa ctcgggaccg ttgcccgatc ctatcgaaac ggcaatcaaa
     3601 aaaggcgatt taaccgtcgg tgcagtgctg tccggcaacc gtaactttga aggccgtatc
     3661 catccgctgg ttaaaaccaa ctggctggcc tcgccgccgc tggtggttgc ctatgcgctg
     3721 gcgggaaata tgaatatcaa cctggcttct gagcctatcg gccatgatcg caaaggcgag
     3781 ccggtatttc tgaaagatat ctggccatcg gcacaagaaa ttgcccgtgc ggtagaccaa
     3841 gtctccacag aaatgttccg caaagagtac gcagaagttt ttgaaggcac agccgagtgg
     3901 aaggaaatta acgtcacacg atccgatacc tacggttggc aggaggattc aacctatatt
     3961 cgcttatcgc ctttctttga tgaaatgcag gcaacaccag cacctgtgga agatattcac
     4021 ggtgcgcgga tcctcgcaat gctgggggat tcagtcacca ctgaccatat ctctccggcg
     4081 ggcagtatta agcccgacag cccggcgggt cgatatctac aaggtcgggg tgttgagcgg
     4141 aaagacttta actcctatgg ttcgcggcgt ggtaaccatg aagtgatgat gcgcggcacc
     4201 ttcgccaata ttcgcatccg taatgaaatg gtgcctggcg ttgaaggggg gatgacgcgc
     4261 catttacctg acagcgatgt cgtctctatt tatgatgccg caatgcgcta taagcaggag
     4321 caaacgccgc tggcggtgat tgccgggaaa gagtatggat caggctccag ccgtgactgg
     4381 gcggcaaaag gtccgcgtct gcttggtatt cgtgttgtga ttgccgaatc gtttgaacga
     4441 attcaccgtt cgaatttaat aggcatgggc atcctgccgc tggaatttcc acaaggtgtg
     4501 acgcgtaaaa cgttagagct taccggggaa gagaagattg atattggtga tctgcaaaat
     4561 ctacaacccg gcgcgacggt tccggtgatg cttacgcgcg cggatggtag ccaggaagtc
     4621 gtaccctgcc attgtcgtat cgacaccgcg acggagttga cctactacca gaacgacggc
     4681 attttgcatt atgtcattcg taatatgttg aagtaacgag tatttgcttg ccggttattt
     4741 tgcttccggc aagcaaatta attacacaat gcaagagggt tatttgttca gcaaatgacc
     4801 cattttctcg gctttggtat cgagataatg ttcgttattg gggttacgac ctacaatcaa
     4861 cggtacgcgt tcaataatat taatccctgc ttcggtcaga atttcgactt ttttcgggtt
     4921 attggttaac aagcggactt cattgacgcc aaggagtttg aacatatcag cgcaaagagt
     4981 gaagtcgcgc tcatcagcgg cgaagcctaa ctggtggtta gcctctacag tatcgtaacc
     5041 ttgatcctgc agtgcgtaag cgcggatttt attcagcaga ccaatgttac gaccttcctg
     5101 acggtgatac agcaaaatac cacggccttc ctcggcaatt tgcgtcaatg ccgcttcgag
     5161 ctggaagcca caatcgcagc gcaagctgaa cagggcgtca ccggtcagac attcggaatg
     5221 gacgcgcgca agtaccgggg tatgcccgga aatatcgcca tagactagcg cgacatgatc
     5281 gtgtccggtt gccagttctt caaatcccac catcaggaaa tcgccccatg gggttggcag
     5341 tttggcttct gccacacgtt taagctgcat gaaattctcc agataatgct ggttctgtat
     5401 tggcttattt tgccataacg agaaggggtt cacctaatca cgacgcgtcg atcgttcacg
     5461 gaatggcaca aatctgtcaa tttttcctgg aactggcatt ttcagttatg attgtgggac
     5521 ttatcaaaaa ggagaggcca tgcgttcgat tgccagacgt accgcagtgg gagctgcact
     5581 attgcttgtc atgccagtag ccgtatggat ttctggctgg tgttggcaac ctggagaaca
     5641 aagttggtta ctaaaagcgg ctttttgggt tactgaaact gtcacccagc cctggggcgt
     5701 cattacacat ttgattttat tcggctggtt tctctggtgt ctgcgttttc gcattaaggc
     5761 tgccattatg ttatttgcca ttctggcggc ggcaatcctt atgggacaag gcgttaaatc
     5821 ctggatcaaa gacaaagtcc aggaaccacg accttttgtt atctggctgg aaaaaacaca
     5881 tcatattccg gttgatgagt tctacacttt aaagcgagca gaacgcggaa atctggtgaa
     5941 agaacagttg gctgaagaga aaaatatccc acaatatttg cgttcacact ggcagaaaga
     6001 gacggggttt gcctttcctt ccggtcacac gatgtttgct gccagttggg cactgctggc
     6061 cgttggtttg ctgtggccgc gtcggcgaac gttaaccatt gctatcttgt tggtctgggc
     6121 aacgggagtc atgggaagcc gcctgctgct cgggatgcat tggccacgcg atctggttgt
     6181 agctacgttg atttcgtggg cgctggtggc ggtggcaacg tggcttgcgc aacgaatttg
     6241 tgggccatta acaccacctg cggaagaaaa tcgcgaaata gcgcaacgag aacaagaaag
     6301 ttaacgctgg ttgattttcc gaatttagcc cttaaatcat caacaatgcg cgtggatgcc
     6361 atttcgcaga cggcgcgaaa atggtacttt aaagggctat tgcggtaagt tgaccataat
     6421 ttattcgctc taaccacata acgggaagta atgtgaaata tttactcatt ttcttactgg
     6481 tgttagcgat cttcgtgatt tcggtcacgt tgggtgcgca gaacgatcaa caggtgacgt
     6541 ttaattatct gttagcgcaa ggggagtacc gtatttccac attgctggcg gtattgtttg
     6601 ctgcggggtt tgctatcggt tggttgattt gcggcctgtt ctggctgcga gttcgtgttt
     6661 ccctggcgcg cgctgaacgt aaaataaagc gactggaaaa ccagctttca cccgcgactg
     6721 acgtggctgt agcgccgcac tcgtctgcgg cgaaggaata actttctatg ctggagttgt
     6781 tgtttctgct tttgcctgta gccgctgcct atggctggta tatgggccgc agaagtgcgc
     6841 aacaaaacaa gcaagatgaa gccaaccgct tgtcgcgtga ttacgtagcg ggggttaact
     6901 tcctgcttag taatcaacag gataaagcgg tagacctgtt tctcgatatg cttaaagagg
     6961 atacaggtac cgttgaagcc caccttacgc tcggaaacct gttccgttcg cgtggcgaag
     7021 ttgatcgcgc tattcgcatc catcagaccc taatggaaag cgcctcgctg acttatgaac
     7081 agcggctttt ggcgattcaa caactggggc gtgattacat ggctgccggg ttatacgacc
     7141 gcgcggaaga catgttcaat cagctgacag atgaaactga cttccgcatc ggcgcgctgc
     7201 aacaactgct acaaatttac caggctacca gcgagtggca gaaagcaatt gatgttgccg
     7261 aacgcctggt gaagctgggt aaagataaac agcgcgtcga aattgcccat ttctactgtg
     7321 agttagctct gcagcatatg gccagcgacg atctcgatcg tgccatgact ttgctaaaaa
     7381 aaggtgcggc ggcagataaa aacagcgctc gcgtatccat catgatggga cgcgtgttta
     7441 tggcgaaagc agaatacgcc aaagccgtcg aaagtttgca acgtgtcata tcccaggaca
     7501 gagaactggt cagcgaaacg ctggaaatgc tgcaaacatg ctatcagcag ttgggtaaaa
     7561 ctgccgaatg ggcagaattc ctgcagcgcg cggtggaaga gaacaccggt gccgatgctg
     7621 aattgatgct tgcggatatc atcgaagcgc gcgacggtag tgaggctgca caggtctata
     7681 ttacgcgcca gcttcagcgt catccgacca tgcgtgtgtt ccataaatta atggattacc
     7741 acttaaatga agcagaagaa gggcgtgcca aagagagtct gatggtgctg cgtgacatgg
     7801 ttggcgagaa ggtacgtagt aagcctcgtt atcgctgcca gaaatgtggt tttaccgcat
     7861 acaccctcta ctggcattgt ccgtcttgtc gggcctggtc aaccattaaa ccaattcgcg
     7921 gtcttgatgg cctgtaattt ttaaaaaaat ccgactttag ttacaacata ctaattattg
     7981 atcttctatt gtgctccggc accgacggcg cgcaaaagcc tgccagagga gaaatcgcaa
     8041 ctgttaattt tttatttcca cgggtagaat gctcgccgtt tacctgtttc gcgccacttc
     8101 cggtgcccat catcaagaag gtctggtcat gacgttaact gcttcatctt cttcccgcgc
     8161 tgttacgaat tctcctgtag ttgttgccct tgattatcat aatcgcgatg ccgcgatggc
     8221 ctttgtcgac aagatcgacc cacgcgattg tcgtctgaag gtcggcaaag agatgtttac
     8281 attgtttggg ccacagtttg tgagcgaact tcaacagcgt ggttttgata tctttcttga
     8341 cctgaaattc cacgatattc ccaacactgc agcgcacgct gtcgctgctg cggctgactt
     8401 aggcgtgtgg atggtgaatg ttcatgcctc cggtggggcg cgtatgatgg ccgcagcgcg
     8461 tgaggcactg gttccgtttg gcaaagatgc accgcttttg attgctgtga cagtgttgac
     8521 cagcatggaa gcctgcgacc tggccgatct tggcgtgaca ctgtcacctg cagattatgc
     8581 agaacgtctg gcggcactga cgcaaaaatg tggccttgat ggtgtggtgt gttctgctca
     8641 ggaagctgtg cgctttaaac aggtattcgg tcaggagttc aaactggtta cgccgggcat
     8701 tcgtccgcag gggagtgatg ctggcgacca gcgccgcatt atgacgccag aacaggcgtt
     8761 ggcggctggt gttgattata tggtgattgg tcgcccggta acgcaatcgg tagatccagc
     8821 gcagacgctg aaagcgatca acgcctcttt acagcggagt gcatgatgag tgattccaac
     8881 agccgtctgg tctactcaac ggaaaccgga cgtattgatg agccaaaagc ggcccctgta
     8941 cgccctaaag gcgacggtgt ggtgcgtatt cagcgtcaga ccagtggacg taaaggtaag
     9001 ggcgtttgcc tgattaccgg tgtcgatctc gatgatgccg aactgacgaa actggcagcg
     9061 gaactgaaga aaaaatgcgg ctgcggcgga gcagttaaag atggaattat tgaaatccag
     9121 ggcgataagc gtgatttatt aaaatcactg cttgaagcga aagggatgaa ggtaaaactc
     9181 gcaggcggct aacataaaaa gccacggata tatccgtggc tttcgaatat tttactgtgt
     9241 gtattattca gttatttcgt actgaacaga atcaagcgta ttataattgc gagtcgataa
     9301 tcgacattta tttacgatta tttaccgacc tggtgaccaa taacaccacc gacggctgca
     9361 ccacctaatg tacccaacgt actgccatcg gtcagtactg caccgcctaa tgcccctgca
     9421 cctgcgccga ttgcggtgtt gcggtcccgt ttagaccagt tagaacaggc actcagagac
     9481 attgccaggg taattgccag aacagccgcg gtcatttttt tgctcgttac aaacataata
     9541 ctctctcctg aatttatgat tcacggaagt aagctctcta taactataat agctaagaat
     9601 aagccaggtg aaattattcc gtgaaatccg ctcgcgcgca gggttatatc acgcaggtga
     9661 taatgacttc ctgttatatc gctgataata attttatatc ttgagagtgt taataacagg
     9721 taaatagtct taattatcaa ccaggaatca tcttagagcg gatgatttgc caaactgcaa
     9781 atcatccgta gagaagggaa atggttaaat gtcaacgacg tgaacggtga gtttactttg
     9841 ttcaagatgc atcagatcgc tggcgcgtat tttcgaatcg gtaatcacgc gattgaatcg
     9901 ctcaacgggg ccgatggagt atgaatgtac agcaccaaat ttcgagctgt cggtcaggac
     9961 tatcgcctcg cactcttttt ccagcacggc attgaccaca tcggtacgca tcatatcgcg
    10021 accggtaaac ccagtttcag gttgccagcc atcaatacca ataaatgctt tgctgaaatg
    10081 cacctgttgg atgcactggc gtgtcaaagg gccaaccata ctttcgcttt ttttctggta
    10141 cacgccaccg agcaaaatga cttcacaagg cgcgtctttc agcaaatgcg cgatgtagct
    10201 gctgaccgtg atgatagtga catttttctt ctgctcgccc agagtccggg cgagcagggc
    10261 attgctgcta ccattttcga taaagatggt ttcgcccggt tgaaccagtg acgcggcaaa
    10321 ctcggcgagt tcacgcttca gcgtatagtt gctcatcata cgggtttcga cgtcatcact
    10381 atcaagcgaa actgcaaaac catgtgcacg tcggaggtaa ctcagttttt cgagggtgtt
    10441 gagatcctgg cgaatggtaa cttcagaaac tccagtggct tttgccagat cggttacgct
    10501 aacctgaccc tggtcaatga ccatctgtag aatagtttgt tgtcgggaa
//
LOCUS       DAHHTG010000140        10456 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.141, whole genome shotgun sequence.
ACCESSION   DAHHTG010000140 DAHHTG010000000
VERSION     DAHHTG010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10456)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10456)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10456
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.141"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            113..2254
                     /locus_tag="NBI74_003804"
     CDS             113..2254
                     /locus_tag="NBI74_003804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent siderophore receptor"
                     /protein_id="HCD1934079.1"
                     /translation="MAMFTPSFSGLKGRALFSLLFAAPMIHATDSVTTKDGETITVTA
                     DANTATEATDGYQPLSTSTATLTDMPMLDIPQVVNTVSDQVLENQNATTLDEALYNVS
                     NVVQTNTLGGTQDAFVRRGFGANRDGSIMTNGLRTVLPRSFNAATERVEVLKGPASTL
                     YGILDPGGLINVVTKRPEKTFHGSVSATSSSFGGGTGQLDITGPIEGTQLAYRLTGEV
                     QDEDYWRNFGKERSTFIAPSLTWFGDNATVTMLYSHRDYKTPFDRGTIFDLTTKQPVN
                     VDRKIRFDEPFNITDGQSDLAQLNAEYHLNSQWTARFDYSYSQDKYSDNQARVTAYDA
                     TTGTLTRRVDATQGSTQRMHATRADLQGNVDIAGFYNEILGGVSYEYYDLLRTDMIRC
                     KKAKDFNIYNPVYGNTSKCTTVSASDSDQTIKQESYSAYAQDALYLTDNWIAVAGIRY
                     QYYTQYAGKGRPFNVNTDSRDEQWTPKLGLVYKLTPSVSLFANYSQTFMPQSSIASYI
                     GDLPPESSNAYEVGAKFELFDGITADIALFDIHKRNVLYTESIGDETIAKTAGRVRSR
                     GVEVDLAGALTENINIIASYGYTDAKVLEDPDYAGKPLPNVPRHTGSLFLTYDIHNMP
                     GNNTLTFGGGGHGVSRRSATNGADYYLPGYFVADAFAAYKMKLQYPVTLQLNVKNLFD
                     KTYYTSSIATNNLGNQIGDPREVQFTVKMEF"
     gene            complement(2318..4210)
                     /gene="parE"
                     /locus_tag="NBI74_003805"
     CDS             complement(2318..4210)
                     /gene="parE"
                     /locus_tag="NBI74_003805"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HCD1934080.1"
                     /translation="MTQTYNADAIEVLTGLEPVRRRPGMYTDTTRPNHLGQEVIDNSV
                     DEALAGHAKRVDVILHADQSLEVIDDGRGMPVDIHPEEGVPAVELILCRLHAGGKFSN
                     KNYQFSGGLHGVGISVVNALSKRVEVNVRRDGQIYNIAFENGEKVQDLQVVGTCGKRN
                     TGTSVHFWPDETFFDSPRFSVSRLTHVLKAKAVLCPGVEITFKDEINNTEQRWCYQDG
                     LNDYLAEAVNGLPTLPEKPFIGNFAGDTEAVDWALLWLPEGGELLTESYVNLIPTMQG
                     GTHVNGLRQGLLDAMREFCEYRNILPRGVKLSAEDIWDRCAYVLSVKMQDPQFAGQTK
                     ERLSSRQCAAFVSGVVKDAFILWLNQNVQAAELLAEMAISSAQRRMRAAKKVVRKKLT
                     SGPALPGKLADCTAQDLNRTELFLVEGDSAGGSAKQARDREYQAIMPLKGKILNTWEV
                     SSDEVLASQEVHDISVAIGIDPDSDDLSQLRYGKICILADADSDGLHIATLLCALFVK
                     HFRALVKHGHVYVALPPLYRLDLGKEVYYALTEEEKEGVLEQLKRKKGKPNVQRFKGL
                     GEMNPMQLRETTLDPNTRRLVQLTIDDEDDQRTDAMMDMLLAKKRSEDRRNWLQEKGD
                     MAEIEV"
     gene            complement(4239..4820)
                     /gene="yqiA"
                     /locus_tag="NBI74_003806"
     CDS             complement(4239..4820)
                     /gene="yqiA"
                     /locus_tag="NBI74_003806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase YqiA"
                     /protein_id="HCD1934081.1"
                     /translation="MSTLLYLHGFNSSPRSAKASLLKNWLAEHHPDVEMIIPQLPPYP
                     SDAAELLESIVLEHGGDSLGIVGSSLGGYYATWLSQCFMLPAVVVNPAVRPFELLTDY
                     LGQNENPYTGQQYVLESRHIYDLKVMQIDPLEAPDLIWLLQQTGDEVLDYRQAVAYYA
                     SCRQTVIEGGNHAFTGFEDYFNPIVDFLGLHHL"
     gene            complement(4820..5647)
                     /gene="cpdA"
                     /locus_tag="NBI74_003807"
     CDS             complement(4820..5647)
                     /gene="cpdA"
                     /locus_tag="NBI74_003807"
                     /EC_number="3.1.4.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3',5'-cyclic-AMP phosphodiesterase"
                     /protein_id="HCD1934082.1"
                     /translation="MESLLTLPLAGEARVRILQITDTHLFAQKHEALLGVNTWESYQA
                     VLEAIRPHQHEFDLIVATGDLAQDQSSAAYQHFAEGIASFRAPCVWLPGNHDFQPAMY
                     SALQDAGISPAKRVFIGEQWQILLLDSQVFGVPHGELSEFQLEWLERKLADAPERHTL
                     LLLHHHPLPAGCSWLDQHSLRNAGELDTVLAKFPHVKYLLCGHIHQELDLDWNGRRLL
                     ATPSTCVQFKPHCSNFTLDTIAPGWRTLELHADGTLTTEVHRLADARFQPDTASEGY"
     gene            complement(5672..6094)
                     /locus_tag="NBI74_003808"
     CDS             complement(5672..6094)
                     /locus_tag="NBI74_003808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1249 family protein"
                     /protein_id="HCD1934083.1"
                     /translation="MKRYTPDFPEMMRLCEMNFSQLRRLLPRNDAPGETVSYQVANAQ
                     YRLTIVESTRYTTLVTIEQTAPAISYWSLPSMTVRLYHDAMVAEVCSSQQIFRFKARY
                     DYPNKKLHQRDEKHQINQFLADWLRYCLAHGAMAIPVY"
     gene            complement(6095..6724)
                     /gene="nudF"
                     /locus_tag="NBI74_003809"
     CDS             complement(6095..6724)
                     /gene="nudF"
                     /locus_tag="NBI74_003809"
                     /EC_number="3.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribose diphosphatase"
                     /protein_id="HCD1934084.1"
                     /translation="MLKPDNLPVTFGKNDVEIIARETLYRGFFSLDLYRFRHRLFNGQ
                     MSHEVRREIFERGHAAVLLPFDPVRDEVVLIEQIRIAAYDTSETPWLLEMVAGMIEEG
                     ESVEDVARREAIEEAGLIVKRTKPVLSFLASPGGTSERSSIMVGEVDATTASGIHGLA
                     DENEDIRVHVVSREQAYQWVEEGKIDNAASVIALQWLQLHHQALKNEWA"
     gene            6929..8410
                     /gene="tolC"
                     /locus_tag="NBI74_003810"
     CDS             6929..8410
                     /gene="tolC"
                     /locus_tag="NBI74_003810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane channel protein TolC"
                     /protein_id="HCD1934085.1"
                     /translation="MKKLLPILIGLSLSGFSSLSQAENLMQVYQQARLSNPELRKSAA
                     DRDAAFEKINEARSPLLPQLGLGADYTYSNGYRDANGINSNATSASLQLTQSIFDMSK
                     WRALTLQEKAAGIQDVTYQTDQQTLILNTATAYFNVLNAIDVLSYTQAQKEAIYRQLD
                     QTTQRFNVGLVAITDVQNARAQYDTVLANEVTARNNLDNAVEQLRQITGNYYPELAAL
                     NVENFKTDKPQPVNTLLKEAEKRNLSLLQARLSQDLAREQIRQAQDGHLPTLDLTAST
                     GISDTSYSGSKTRGAAGTQYDDSNMGQNKVGLSFSLPIYQGGMVNSQVKQAQYNFVGA
                     SEQLESAHRSVVQTVRSSFNNINASISSINAYKQAVVSAQSSLDAMEAGYSVGTRTIV
                     DVLDATTTLYNAKQELANARYNYLINQLNIKSALGTLNEQDLLALNNALSKPVSTNPE
                     NVAPQTPEQNAIADGYAPDSPAPVVQQTSARTTTSNGHNPFRN"
     gene            8558..9229
                     /locus_tag="NBI74_003811"
     CDS             8558..9229
                     /locus_tag="NBI74_003811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417509.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 family protein"
                     /protein_id="HCD1934086.1"
                     /translation="MKRTKSIRHASFRKNWSARHLTPVALAVATVFMLAGCEKSDETV
                     SLYQNADDCSAANPGKSAECTTAYNNALKEAERTAPKYATREDCVAEFGEGQCQQAPA
                     QAGMAPENQAQAQQSSGSFWMPLMAGYMMGRLMGGGAGFAQQPLFSSKNPASPAYGKY
                     TDATGKNYGAAQPGRTMTVPKTAMAPKPATTTTVTRGGFGESVAKQSTMQRSATGTSS
                     RSMGG"
     gene            9235..10395
                     /locus_tag="NBI74_003812"
     CDS             9235..10395
                     /locus_tag="NBI74_003812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HCD1934087.1"
                     /translation="MERVSITERPDWREKAHEYGFNFHTMYGEPYWCEDAYYKLTLSQ
                     VEKLEEVTAELHQMCLKVVEKVIASDELMTKFRIPKHTWSFVRQSWLTHQPSLYSRLD
                     LAWDGTGEPKLLENNADTPTSLYEAAFFQWIWLEDQLNAGNLPEGSDQFNSLQEKLID
                     RFVELREQYGFQLLHLTCCRDTVEDRGTIQYLQDCATEAEIATEFLYIDDIGLGEKGQ
                     FTDLQDQVISNLFKLYPWEFMLREMFSTKLEDAGVRWLEPAWKSIISNKALLPLLWEM
                     FPNHPNLLPAYFAEDDHPQMEKYVVKPIFSREGANVSIIENGKTIEAAEGPYGEEGMI
                     VQQFHPLPKFGDSYMLIGSWLVNDQPAGIGIREDRALITQDMSRFYPHIFVE"
BASE COUNT         2634 a         2780 c         2587 g         2455 t
ORIGIN      
        1 tgattctcaa ttacatatcc gatggcgaaa taacaaacgg atagtgctta gtgagcaata
       61 agaaacaatt ttcactcgcg acaatccgcg agtttttggg ataacagact aaatggctat
      121 gttcacacct tcattctcag gactcaaagg tcgggcgctc ttttcactgc tttttgcggc
      181 accgatgatt catgcaacag actctgtaac gaccaaagat ggcgaaacaa tcactgttac
      241 agcagatgca aataccgcaa ctgaggcaac cgatggttat caacctctga gcacctctac
      301 ggcgacatta accgatatgc cgatgctgga tatcccgcag gtggtcaata cggttagcga
      361 tcaggttctg gaaaaccaga atgcgacaac gctggatgag gcgctttata acgtcagtaa
      421 cgtggtacag accaatacat taggcggaac tcaggacgcc tttgtacgcc gtgggtttgg
      481 cgcaaaccgg gatggctcca tcatgaccaa cggtctgcga accgtacttc ctcgcagttt
      541 caacgccgcc acagaacgtg tggaagtgct aaaaggcccg gcctccacgc tgtatggcat
      601 tctcgatcct ggcggactga ttaacgtcgt gaccaagcgc ccggaaaaaa cattccatgg
      661 ttcggtttca gccacctcct ccagttttgg tggcggcact gggcaacttg atatcacagg
      721 tcccattgaa ggcactcagc tggcgtatcg ccttaccggg gaagtgcagg atgaagatta
      781 ctggcgaaac ttcggtaaag agcgcagtac atttattgcc ccgtcactca catggtttgg
      841 tgataatgca acagtaacca tgctctattc ccatcgggac tataaaactc cgttcgatcg
      901 tggaacgatt ttcgacctta cgacgaaaca gcccgtaaac gttgatcgaa aaatacgttt
      961 tgacgaaccg tttaatatta cagatggtca gtccgatctg gcgcaactca acgcagaata
     1021 tcatctcaat agccagtgga cagcacgctt tgattacagc tacagccagg ataaatacag
     1081 cgataatcag gcgcgtgtta ccgcatatga tgcaacgaca ggaacactga cacggcgtgt
     1141 tgatgcaact cagggctcta cccagcgtat gcatgctact cgtgcggatc tgcaagggaa
     1201 tgttgatatt gccggattct ataatgagat tctgggtggg gtgtcatatg aatattatga
     1261 tcttctgcgt acagatatga ttcgctgtaa aaaagctaaa gatttcaata tttacaaccc
     1321 tgtttatggt aataccagca aatgtacaac ggtttcggcg tcggacagtg atcagacgat
     1381 caaacaggag agctactcag cgtatgcaca ggacgcactc tatctgaccg ataactggat
     1441 tgccgtcgcc gggatccgct atcagtatta cacgcagtat gcgggtaaag gccgtccttt
     1501 taatgtcaat actgacagcc gcgatgaaca atggactccc aaactggggt tggtctacaa
     1561 actgacgcca tcggtatcct tatttgccaa ttattcgcaa acatttatgc cgcagtcgtc
     1621 aattgccagc tacattggcg atcttccacc ggaatcatct aatgcttacg aagtcggggc
     1681 aaaattcgag ctattcgatg gtatcaccgc agatattgcg ctgtttgata tccataaacg
     1741 taatgtgttg tataccgaaa gtattggtga tgaaaccatc gccaaaacgg caggccgtgt
     1801 tcgttcaaga ggggtagaag tcgaccttgc gggagcatta actgaaaaca ttaatatcat
     1861 tgccagctac ggctataccg atgctaaggt tctggaggat cctgattatg cagggaaacc
     1921 attgccgaat gttcctcgtc ataccggttc gctattcctg acctatgata ttcataacat
     1981 gccaggcaat aatacgctga catttggcgg tggtggacat ggtgtaagcc gtcgttcggc
     2041 aaccaatggg gctgactatt atctgccagg ctattttgtt gccgatgcct tcgccgcata
     2101 caaaatgaaa ttgcagtatc cggtcacact gcaattaaac gtcaaaaacc tgtttgataa
     2161 aacgtattac acctcttcca tcgccacaaa taatctgggg aatcagattg gcgatccgcg
     2221 tgaagtgcaa ttcacggtga aaatggaatt ttgatcaaaa aagcccggta tacacttgta
     2281 taccgggcta tgttttattc ttctggcggg aggattatca gacctcaatc tctgccatgt
     2341 cgcctttttc ttgcaaccag ttgcggcgat cttccgagcg tttcttcgcc agcagcatat
     2401 ccatcatcgc gtcagtacgc tgatcgtctt catcatcgat agtcaactgc accagacggc
     2461 gagtgttcgg atcaagcgtg gtttcgcgca attgcatcgg gttcatttcc ccgagacctt
     2521 taaaacgctg gacgtttggc ttgcctttct tgcgttttaa ttgctcaagt acgccctctt
     2581 tctcttcttc cgtcagcgcg taataaacct ctttcccgag gtcaagacgg tagagcggtg
     2641 gcagtgcaac gtaaacgtga ccgtgtttca ccaatgcgcg gaagtgtttt acaaacaaag
     2701 cgcagagcag cgtggcaatg tgcagaccat cggagtcagc atccgccagg atacagatct
     2761 tgccgtaacg aagctggctc aaatcgtcgc tgtcaggatc gataccgata gctaccgaaa
     2821 tatcgtgcac ttcctgcgaa gccagcactt cgtcggaaga gacttcccag gtgttaagga
     2881 tcttaccttt cagtggcatg atcgcctgat attcgcgatc gcgcgcctgc ttggcagatc
     2941 cgcctgcgga gtcaccttcc acaaggaata gttcggtacg gttaagatcc tgcgcggtac
     3001 agtcagccaa tttaccaggc agcgccgggc cgctggttag ctttttacgc accacttttt
     3061 tagccgcacg catacgacgc tgggcgctgg aaatcgccat ctctgccaac agctccgccg
     3121 cctgaacgtt ttggttcagc cacaggatga aggcatcttt caccacgccc gaaacgaatg
     3181 ccgcacactg acgcgaagag agacgttctt tggtctgccc ggcaaactgc ggatcctgca
     3241 tttttactga cagcacatag gcgcagcgat cccagatatc ttccgccgac agctttacac
     3301 cacgcggcag aatgttgcgg tattcacaga actcacgcat cgcgtccagc aggccctgac
     3361 gcaggccatt aacatgggta ccgccctgca tcgttgggat caggttgacg tagctttcgg
     3421 tcagcagttc accgccttcc ggcagccaca gtagcgccca gtccaccgct tcagtatcgc
     3481 cagcgaaatt accgataaac ggtttttctg gcagcgtcgg taaaccgttt accgcttccg
     3541 ccaggtaatc attcagaccg tcctgatagc accagcgctg ttcggtgttg ttgatctcat
     3601 ctttaaaagt gatctcaacg cccgggcaca gtaccgcttt ggctttcagc acatgcgtca
     3661 ggcgtgaaac agaaaaacgc gggctgtcaa agaaggtttc atccggccag aagtgcacgc
     3721 tggtaccggt attgcgttta ccgcaagtgc cgacaacctg taaatcttgc accttttcgc
     3781 cattttcaaa ggcgatgtta tagatctgac catcgcggcg tacgttaact tctacgcgct
     3841 tcgacagggc gttaactacc gaaatcccca cgccatgcag gccgccagag aactggtaat
     3901 ttttgttaga gaatttaccg cccgcgtgca gacggcaaag aatcagttca accgccggta
     3961 ccccctcttc cgggtgaata tctaccggca tcccgcgccc atcgtcaatc acttctaacg
     4021 actggtcggc atgtaagatt acatccacgc gttttgcgtg gcccgccagt gcttcatcga
     4081 cactgttatc aataacttct tgccccaaat ggttagggcg agtggtatcg gtatacatcc
     4141 ccggacggcg gcgaaccggc tcaagcccgg tgagtacctc aatggcatca gcgttataag
     4201 tttgcgtcat ggtttaagtt agcaattcga gttgatcgtc agagatggtg cagaccaaga
     4261 aaatcgacga tcgggttgaa ataatcttcg aagcccgtga atgcgtggtt gccgccttct
     4321 atgacagtct ggcggcagga agcgtagtac gccaccgcct ggcggtaatc cagcacttca
     4381 tctcccgtct gttgcagcag ccagatcaaa tccggcgctt ccagcgggtc aatctgcatg
     4441 actttgagat cgtaaatatg gcgtgactct agcacatatt gctgcccggt gtaggggttc
     4501 tcgttctgac cgagatagtc cgtcagcagt tcaaacgggc gcaccgccgg gttgaccacc
     4561 actgcgggca gcataaaaca ttgcgacaac caggtggcgt aatatccccc cagtgacgaa
     4621 ccgacaatac ccagcgaatc gccgccatgt tccaggacaa tggattccag cagttctgcc
     4681 gcgtcggaag gatacggcgg cagctgcgga atgatcatct caacgtcagg gtgatgttcc
     4741 gccagccagt tttttaacaa gctcgctttt gcagagcgcg gcgagctgtt gaaaccgtgt
     4801 aaataaagaa gcgtagacat cagtagcctt ctgaagcggt atcaggttgg aagcgtgcgt
     4861 ccgccaggcg atgcacctcg gtggtcagcg tgccatcagc atgtaactca agagtacgcc
     4921 agccgggcgc gatggtatcc agcgtaaagt tggaacagtg cggcttaaac tgcacacagg
     4981 tcgacggcgt tgccagcagg cggcgaccgt tccagtcgag atccagctcc tgatgaatat
     5041 gaccgcacag caagtatttg acgtgcggaa acttcgccag cacggtatcc agttcgcccg
     5101 cgttacgcag gctgtgttga tcaagccaac tacaacccgc aggtagcgga tgatgatgca
     5161 gcagcagcaa cgtatggcgt tctggcgcat cggccagttt acgttccagc cactcaagct
     5221 gaaactcgct cagctcaccg tgcggcacgc caaacacctg gctatccagc aacaggattt
     5281 gccattgctc accaataaac acccgcttcg ccggggagat acccgcatcc tgtaacgcac
     5341 tgtacatcgc gggctggaaa tcgtggttgc ccggcagcca gacgcagggc gcacgaaaac
     5401 ttgcgatgcc ttcagcgaaa tgctgatagg ccgcagagga ttgatcctgc gctaaatcac
     5461 ctgtcgcgac aatcaggtcg aattcgtgct ggtgtggccg aatcgcctcc agcaccgcct
     5521 ggtagctttc ccaggtgttt acccctaaca gggcttcgtg cttttgtgca aacaggtgag
     5581 tgtcggtaat ttgtaaaatc ctgactctgg cctcaccagc cagaggaagg gttaacaggc
     5641 tttccaaatg gtgtccttag gtttcacgac gctaataaac cggaatcgcc atcgctccat
     5701 gtgctaaaca gtatcgcaac cagtccgcta aaaactgatt aatttgatgc ttttcgtcgc
     5761 gttgatgcaa ctttttatta ggataatcat accgcgcttt gaagcgaaaa atctgctggc
     5821 ttgaacacac ttcagccacc atcgcgtcat gatacagacg caccgtcatt gacggaaggc
     5881 tccagtaact gatcgcgggc gcagtctgtt ctattgtcac cagggtagtg tatcgggtcg
     5941 attccacaat cgtcagccga tattgtgcgt ttgccacctg atagcttaca gtttcgccgg
     6001 gtgcgtcatt gcgcggtaac aaacggcgca attgtgaaaa gttcatctcg cacaggcgca
     6061 tcatttcagg aaagtcaggt gtgtaacgct tcatttatgc ccactcattt tttaacgctt
     6121 gatgatgcag ctgcagccat tgcaaagcga tgaccgacgc tgcgttgtcg attttcccct
     6181 cttctaccca ctggtatgcc tgttcccggc ttaccacatg aacgcgaata tcttcgtttt
     6241 catcagccag accgtgaata ccgcttgcgg tcgtggcgtc cacttcgccc accataattg
     6301 acgaacgctc actggtgccc cccgggcttg ccaggaaact taacaccggt ttggtccgtt
     6361 tgactatcag tcccgcctct tcaatcgctt cgcgacgggc aacatcttcc acactttcac
     6421 cctcttcaat catcccggca accatctcca gtagccaagg ggtttcgctg gtgtcgtacg
     6481 cggcaatccg aatctgctca atcagcacaa cttcatcacg cactgggtca aagggtagca
     6541 agactgcggc gtgaccgcgc tcaaaaattt cccgccgtac ctcatgactc atttgcccgt
     6601 tgaatagacg atgacgaaat ctataaagat ctaatgaaaa aaagccgcga taaagtgttt
     6661 ctcgtgcaat aatttctaca tcgtttttgc caaatgtaac gggcaggttg tctggcttaa
     6721 gcattgttaa tgtcctggca ctaatagtga attaaatgtg aatttcagcg acgtttgact
     6781 gccgtttgag cagtcatgtg ttaaattgag gcacattaac gccctatggc acgtaacgcc
     6841 aaccttttgc ggtagcggct tctgctagaa tccgcaataa ttttacagtt tgatcgcgct
     6901 aaatactgct tcaccacaag gaatgcaaat gaagaaattg ctccccattc ttatcggcct
     6961 gagcctctct gggttcagtt cgttgagcca ggccgagaac ctgatgcaag tttatcagca
     7021 agcacgcctt agtaacccgg aattgcgtaa gtctgccgcc gatcgtgatg ctgcctttga
     7081 aaaaattaat gaagcgcgca gtccattact gccacagcta ggtttaggtg cagattacac
     7141 ctatagcaac ggctaccgcg acgcgaacgg catcaactct aacgcgacca gtgcgtccct
     7201 gcagttaact caatccattt ttgatatgtc gaaatggcgt gcgttaacgc tgcaggaaaa
     7261 agcagctggg attcaggacg tcacgtatca gaccgatcaa caaaccttga tcctcaacac
     7321 cgcgaccgct tatttcaacg tgttgaatgc gattgacgtt ctttcctata cacaggcgca
     7381 aaaagaagcg atctaccgtc aattagatca aaccacccaa cgttttaacg tgggcctggt
     7441 agcgatcacc gacgtgcaga acgcccgcgc acagtacgat accgtgctgg cgaacgaagt
     7501 gaccgcacgt aataaccttg ataacgcggt agagcagctg cgccagatca ccggtaacta
     7561 ctatccggaa ctggcggcgc tgaatgtcga aaactttaaa accgacaaac cacagccggt
     7621 taacacgctg ctgaaagaag ccgaaaaacg caacctgtcc ctgttacagg cgcgtttgag
     7681 ccaggacctg gcgcgcgagc aaattcgcca ggcgcaggat ggtcacttac cgactctgga
     7741 tttaacggct tctaccggga tttctgacac ctcttatagc ggttcgaaaa cccgtggtgc
     7801 cgctggtacc cagtatgacg atagcaatat gggccagaac aaagttggcc tgagcttctc
     7861 gctgccgatt tatcagggcg gaatggttaa ctcgcaggtg aaacaggccc agtacaactt
     7921 tgttggtgcc agcgagcaac tggaaagcgc gcatcgtagc gtcgtacaaa ccgtacgttc
     7981 ctccttcaac aacattaatg cttctatcag tagcattaac gcctacaaac aagccgtagt
     8041 ttccgctcaa agctcattag acgcgatgga ggcgggttac tcggtcggta cgcgtaccat
     8101 tgttgatgtg ttggatgcga ccaccacgct gtacaacgct aagcaagagc tggcaaatgc
     8161 gcgttataac tacctgatta accagttgaa tattaagtca gctctgggta cgttgaacga
     8221 gcaggatctg ctggcactga acaatgcgct gagcaaaccg gtttccacta atccggaaaa
     8281 cgttgccccg caaacgccgg aacagaatgc tattgctgat ggttatgcgc ctgatagccc
     8341 ggcaccagtc gttcagcaaa catccgcacg cactaccacc agtaacggtc ataacccttt
     8401 ccgtaactga cgacgacgac ggggcttcgg ccccgtctga acgtaaggca acgtaaagat
     8461 acgggttatc tgccgcattc ttcccccttc tcgcttcaat ttcgaccagc catcctctat
     8521 tctgatgggt atttaccact ggtcccggaa gacaaaaatg aaacggacaa aatccatacg
     8581 ccacgcatcg ttccgcaaaa actggagcgc acgccatctg acaccagtcg ctctcgcggt
     8641 tgccactgtt tttatgctgg cgggctgtga aaagagtgat gaaacagtgt ctctctatca
     8701 aaatgctgac gactgttcag ctgcaaaccc aggcaaaagc gccgaatgta ccaccgcgta
     8761 caacaatgcg ctgaaagaag ccgaacgtac tgcgccgaaa tacgccaccc gtgaagactg
     8821 tgttgctgaa tttggtgaag gtcagtgcca gcaggcacca gcccaggctg gcatggcacc
     8881 agaaaaccag gcgcaggccc agcaatccag cgggagtttc tggatgccgc tgatggccgg
     8941 ttacatgatg ggacgtctga tgggcggcgg cgcgggattt gcacagcagc cgctattctc
     9001 ctcgaaaaac ccggccagcc cggcttacgg taaatatacc gacgcgaccg gtaaaaacta
     9061 tggcgcagcc cagccaggcc gcaccatgac cgtaccgaag acggcaatgg caccaaaacc
     9121 ggcgaccact actactgtta cccgtggcgg ttttggtgaa tctgttgcca aacaaagcac
     9181 tatgcagcgt agcgcaaccg gcacctcttc tcgttcaatg ggtggctgat accgatggaa
     9241 agagtcagta ttaccgagcg cccggactgg cgcgaaaaag cccacgaata cggtttcaat
     9301 tttcacacca tgtacggcga gccgtactgg tgtgaagatg cttactacaa gttgaccctc
     9361 tctcaggttg aaaagctgga agaagtcacc gccgaactgc accagatgtg cctgaaagtg
     9421 gtggaaaaag tgatcgccag cgatgagctg atgaccaaat tccgcatacc aaaacacacc
     9481 tggagttttg tgcgccagtc atggctgacg caccagccat cgctttattc gcgtcttgat
     9541 ctggcgtggg atggcactgg cgaacctaaa cttctggaaa ataacgccga tacgccaacg
     9601 tcactatacg aggcggcgtt ctttcagtgg atctggctgg aagatcagct taacgccggt
     9661 aacttgccgg agggcagcga ccagtttaac agtctgcaag aaaagctgat cgatcgcttc
     9721 gttgagctgc gtgaacagta tggcttccag ttgctgcatc tcacctgctg tcgcgacacg
     9781 gtggaagatc gcggaaccat tcagtatttg caggactgcg caacggaagc tgaaatcgcc
     9841 accgagttcc tctacattga cgatatcggg ttaggtgaaa aaggtcagtt cacggattta
     9901 caggatcagg tgatttccaa cctgttcaag ctgtatccgt gggaatttat gttgcgtgag
     9961 atgttctcca ccaagctgga ggatgcaggc gtacgctggc tggaaccggc gtggaagagc
    10021 attatctcca acaaagcgct tctaccgcta ctgtgggaga tgttcccgaa tcacccgaac
    10081 ctgctgcccg cttattttgc ggaagatgat catccgcaaa tggaaaaata tgtggttaaa
    10141 ccgatcttct cccgtgaagg cgcaaacgtg tcgatcattg agaacggcaa aaccattgaa
    10201 gcagcggaag gtccgtatgg cgaagaaggg atgattgttc agcaattcca cccgttaccg
    10261 aaattcggcg acagctatat gctgattggt agctggctgg tgaacgatca acctgccgga
    10321 attggcattc gtgaagaccg tgcattgatc acccaggata tgtctcggtt ttatccacat
    10381 atttttgttg agtaatacag cttaatattg ccccgataag cggggcaatc tcttataatc
    10441 caccattcat aattcc
//
LOCUS       DAHHTG010000141        10415 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.142, whole genome shotgun sequence.
ACCESSION   DAHHTG010000141 DAHHTG010000000
VERSION     DAHHTG010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10415)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10415)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10415
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.142"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            118..564
                     /locus_tag="NBI74_003813"
     CDS             118..564
                     /locus_tag="NBI74_003813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HCD1934088.1"
                     /translation="MAALTASCIDLNIQGNGTYSVLKQLATMALQNGFITDSHQFLQT
                     LLLREKMHSTGFGSGIAVPHGKSACVKQPFVLFARKTQAIDWQASDGEDVNCWICLGV
                     PQSGEEDQVKIIGTLCRKIIHQDFIHQLRQGDTNQVLALLNQTLSS"
     gene            575..2026
                     /locus_tag="NBI74_003814"
     CDS             575..2026
                     /locus_tag="NBI74_003814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIBC"
                     /protein_id="HCD1934089.1"
                     /translation="MESSLRIVAITNCPAGIAHTYMVAEALEQKARSLGYTIKVETQG
                     SSGVENRLSSEEIAAADYVILATGRGLSGDDRARFAGKKVYEIAISQALKNIDQIFSE
                     LPTNSQLFAADSGVKLGKQEVQSGSVMSHLMAGVSAALPFVIGGGILVALANMLVQFG
                     LPYTDMSKGAPSFTWVVESIGYLGFTFMIPIMGAYIASSIADKPAFAPAFLVCYLAND
                     KALLGTQSGAGFLGAVVLGLAIGYFVFWFRKVRLGKALQPLLGSMLIPFVTLLVFGVL
                     TYYVIGPVMSDIMGGLLHFLNTIPPSMKFAAAFLVGAMLAFDMGGPINKTAWFFCFSL
                     LEKHIYDWYAIVGVVALMPPVAAGLATFIAPKLFTRQEKEAASSAIVVGATVATEPAI
                     PYALAAPLPMITANTLAGGITGVLVIAFGIKRLAPGLGIFDPLIGLMSPVGSFYLVLA
                     IGLALNISFIIVLKGLWLRRKAKAAQQELAHEH"
     gene            2016..3086
                     /locus_tag="NBI74_003815"
     CDS             2016..3086
                     /locus_tag="NBI74_003815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001019504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HCD1934090.1"
                     /translation="MNIELLQQLCEASAVSGDEQEVRDILINTLEPCVNEITFDGLGS
                     FVARKGNKGPKVAVVGHMDEVGFMVTHIDESGFLRFTTIGGWWNQSMLNHRVTIRTHK
                     GVKIPGVIGSVAPHALTEKQKQQPLSFDEMFIDIGANSREEVEKRGVAMGDFISPEAN
                     FACWGEDKVVGKALDNRIGCAMMAELLQTVNNPEITLYGVGSVEEEVGLRGAQTSAEH
                     IKPDVVIVLDTAVAGDVPGIDNIQYPLKLGDGPGLMLFDKRYFPNQKLVAALKSCATQ
                     NDLPLQFSTMKTGATDGGRYNVMGGGRPVVALCLPTRYLHANSGMISKTDYDALLILI
                     RDFLTTLTAEKVNAFSQFRQVS"
     gene            3086..4834
                     /locus_tag="NBI74_003816"
     CDS             3086..4834
                     /locus_tag="NBI74_003816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative frv operon regulatory protein"
                     /protein_id="HCD1934091.1"
                     /translation="MLNERQLKIVDLLEQQPHTPGELAQQTGVSGRTILRDIDYLNFT
                     LNGKARISAGGGVGYQLEIFDRRSFFQLLQKHDNDDRLLALLFLNTFTPRAQLASALN
                     LPETWVAERLLRLKQRYERICCLASRPGLGHFIDECEEKRVILLANLLRKDPFLLPLA
                     GVTRESFQQLSTACDNQHRWPLMQGDYLSSLILAIYALRNQLTNEWPEYPSNEIKQIV
                     EQSGLFLGDNAIRTLTGLIEKQHQQAQAISADNVLALLQRVPGITSLNIIDAQLVENI
                     TGHLLRCLAAPVWIAEHRQSSMNNLKAAWPAAFDMSLHFITLLREQFDIPLFDSDLIG
                     LYFACALERHQNERQPIILLSDQNAIATINQFAIERDVLNCRVIIARSLGELVAICEE
                     IEPLLIINNSHYLLDDAVNNCITVKNIITAAGIEQIKHFLATAFIRRQPERFFPVTGS
                     LHYSNVRGESWQEITGQICVQLVAQQHITADEAQRIIARESEGENLIVNRLAIPHCWS
                     EQERRFRGFFIPLAQPIEVNNEIINHVLIACAAADARHELKIFSYLASVLCQHPPETI
                     AGLTGYEGFIALLRQG"
     gene            complement(4936..5979)
                     /locus_tag="NBI74_003817"
     CDS             complement(4936..5979)
                     /locus_tag="NBI74_003817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiG family protein"
                     /protein_id="HCD1934092.1"
                     /translation="MKRNLLSSAIIVAIMALGITGCDDKKAETETPPPANSQPAAPAP
                     EAKPTEAPIAKAKMPAQPVADEQAVFDEKMNVYIKCYNKLQVHVQHSLARYADWLKDF
                     KQGPTGKELHVYGIYSISESSLSDCEKEIKNVVALMPALEPIDGEALNYIDAAVALGN
                     TINEMDKYYTQENYKDDAFAKGKMLHQVFIKNVDAFKPVAESYHAAIQVINDKRQLVE
                     LKNIEQREGKTFHYYSLAVMILAKQINAMISQENVDAETAMKKVAELETLVAQAKEVS
                     KDSMNSLFIGAADRYQIAAKKYARRIRDNVPYSDSDKKKLQDATTNWMVKDSYPQSLR
                     AYNELVGSYNRLR"
     gene            complement(6199..8505)
                     /locus_tag="NBI74_003818"
     CDS             complement(6199..8505)
                     /locus_tag="NBI74_003818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001591275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1934093.1"
                     /translation="MDTWEWYNKYSQELRDAGQEYIPQLIDDFSESVLHLQIEKCDSL
                     LPEMKALNQVAKNPWLEIFIGHWEMRHRLTNYEEGEKVLPDVVALFEKAHREEAKDCP
                     QSICVTQDLSACYENIDGPGWANERMAVCEETIARIDPTWSCFMCLSCEYASALVDSN
                     KTEEALVYIDRQIEAVKASGSEYLEALLATRADILVALQRYDEAREVIDELAKENDYW
                     GRDRINYHLKKLYIMALCGQDDEVWEELPRWSDLTPYSWGMWVKIITVLLPRHPQRNH
                     WQLARIIQKMLEQRSQAGAHRKAVDLAKLQITLALERESVWVAKMAMKLAQQHMPKLR
                     SLVGVDREFLELSEKIAAKEAELLARPVVEVPENSDEETQPDPEKDVEIFRRAYLNTP
                     DNAELLGKLVNALNACNALNESIELLQRYVESHLQEDNGLCFDLLDLLLNRGDQQAIE
                     HLAKLYEQAGLLHMALWFRARVINQQEDWSELESIGQQLLENEKGREWVGVYRMCSWA
                     AQKQEAWDRQLDYLQQLQQLMISREEDIRNLQWDIMATASILQQWDLVRAIAAELEIE
                     LTEGEGFIDEKWGLLNIRFRENYEYRYYYAQRIGPVTARIIEPTYRTDFPQHVDEIVV
                     FDAEMLNTRPEDEEDQKNFTPLYGCLKTIIPTEYGESWFCEGVMPGEEQFNAFREALR
                     ERNWYYWSNSNDDYTLTDSHQGEDAQPLPAFYFVISAPRSVSKGDIDKTLHELTADWK
                     HPLCWWRLAKEAGANEEKHVAISERYGM"
     gene            complement(8507..10342)
                     /locus_tag="NBI74_003819"
     CDS             complement(8507..10342)
                     /locus_tag="NBI74_003819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000105780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HCD1934094.1"
                     /translation="MSTLQLPGSSYSTEVNLNGLIEVLSKHLYSTPVVAVRELVQNGH
                     DAIVRRRIEQPDAPKDNQIRVVADVAKSTITITDTGAGLTESEIHGFLATVGVGYTRM
                     LRQQDDNTGLIGMFGLGFLSAFVLAKEVTVLTTSWQTPDQSWKYHSTDGQKYTVTPHQ
                     ASGTGTQVILTLKEEYSHLASNNLLNRVLSRYCILLHEPVFVGDASEPVNKLQPPWRE
                     VAPEGVTMHRALVQRKNLAFAAQFESSFEPICTIPVVPAGMSDAVGILWIQDGATYGT
                     SDNRNLSLFLRGMLLDDEARELLPPWAGFIGGVIESSKLTPTASREDLQRDETWVAVQ
                     GALKEALISGLSDLAQNQPEIWRRVLMRHNEALLGAALCDDRLFDLLKDRLQVPTSKG
                     ALLAKDLRVNNSIHILLSRDGGFEEMLFHILQRPVARGDRYAVVPFLRRWALLYHCRI
                     IEVGTQTGNEQLFSLAELPEEQVAYLEEHLCDGEQLIISRFEPAVLPLVVTPDREAEL
                     KQILEQDDADKRISTAALMLARQFTSQIQKTKTSSLYINLNNPCVMQLVTALQHHQQP
                     AAALRLLKSLKVILCSSGNKEQQWDLHQALEDFTQVIPVLINQGK"
BASE COUNT         2506 a         2656 c         2484 g         2769 t
ORIGIN      
        1 cgcaaatttt acctttcact ctgctttata ttgtcacgac ttcgtgacat atccgcgctc
       61 atcacaaacc cgccccgcga ttacaattaa ctgaaatatt tttcatggaa tctgataatg
      121 gcagcactta ctgcaagctg tattgacctg aatattcagg gcaatggcac ttattccgtt
      181 ctgaagcagt tggcgacaat ggcgttacaa aacggtttta tcaccgactc acaccagttc
      241 ctgcaaacgt tactactgcg cgaaaaaatg cactccaccg gttttggttc cggcatagcc
      301 gtaccgcacg gtaaaagcgc ctgcgttaaa caaccattcg tgttgttcgc acgtaaaaca
      361 caagccattg actggcaagc cagcgatggc gaagacgtca attgctggat ctgcctcggc
      421 gtaccgcaaa gcggcgaaga ggatcaggtc aaaatcatcg gcacactgtg tcgcaaaatt
      481 atccatcagg attttatcca ccaactgcga caaggcgaca ccaaccaggt gcttgctttg
      541 ttaaatcaaa ccctcagctc ataaggacgt agcgatggag tcatccttac gtattgtcgc
      601 gatcaccaac tgccctgccg ggatcgctca cacctacatg gtggcggaag ccctggaaca
      661 gaaagcccgt tctctcggct ataccattaa agtggaaact caaggatcaa gcggcgttga
      721 aaaccgctta tccagcgaag agattgccgc tgccgattac gtgattctcg ccaccgggcg
      781 tggtctgagc ggcgacgatc gcgcgcggtt tgccgggaag aaggtttatg agattgccat
      841 ctcccaggcg ttgaaaaata tcgaccagat tttcagtgaa ttaccgacaa actcgcagct
      901 ttttgccgca gatagcggcg taaagctcgg caaacaggaa gtacaaagcg gcagcgtgat
      961 gagccacctg atggcgggcg tctctgcggc actgccgttt gtcatcggcg gcggtattct
     1021 ggtggcgctt gccaacatgt tggtgcagtt cggtttgccg tataccgata tgtcgaaagg
     1081 cgcgccgtcg tttacctggg tggttgaatc catcggttat ctcggcttca cctttatgat
     1141 acccatcatg ggcgcttata ttgcctcgtc aattgccgat aaaccggctt tcgctccggc
     1201 gtttctggtt tgctacctgg cgaacgacaa agcgttgctc ggcacccagt caggcgcggg
     1261 tttcctcggc gcggtagtgc tggggctggc gattggctac ttcgtcttct ggtttcgcaa
     1321 agtgcgtctt ggtaaagcat tacaaccgtt gctcggttca atgctgatcc cgttcgttac
     1381 tctgctggtt tttggcgtac tgacttacta cgttatcgga ccggtgatgt ccgacattat
     1441 gggcggtctg ctgcacttcc tgaacaccat ccctccctca atgaaatttg cggcggcgtt
     1501 tctggtcggt gcgatgctgg cgttcgatat gggtggcccc atcaataaaa ccgcctggtt
     1561 cttctgcttc tcactgctgg aaaaacacat ttacgactgg tacgccatcg tcggtgttgt
     1621 cgcgctaatg ccgcccgtcg cggcgggtct tgcgacgttc attgcgccca aactgtttac
     1681 ccgacaggaa aaagaggctg ccagtagcgc tattgtggtc ggtgccactg tcgcaaccga
     1741 accggctatt ccttacgctc tggcagcgcc gttgccgatg attaccgcca atacgctggc
     1801 aggcggcatt accggcgtgc tggtgatcgc tttcggaatc aaacgtctgg cgccaggttt
     1861 gggtatcttt gacccgctga tcggcctgat gtcgccggta ggttcgtttt atctggtgct
     1921 ggcgattggt ctcgcgctga acatttcatt cattatcgtc ctgaaaggat tatggttacg
     1981 ccgcaaagcc aaagccgcgc aacaggagct tgcccatgaa cattgagtta ctgcaacagt
     2041 tgtgcgaagc cagcgccgtt agcggcgatg aacaggaagt tcgcgacatt ctgataaaca
     2101 cgctggaacc ttgcgttaat gagatcacct ttgatggtct gggcagcttt gttgcccgca
     2161 aggggaataa aggtccaaaa gttgccgttg tcgggcatat ggatgaagtc ggctttatgg
     2221 ttacccacat cgacgagagc ggttttctgc gtttcaccac cattggtggc tggtggaatc
     2281 agtcgatgct caaccaccgg gtaaccatac gcacacacaa gggagtgaaa atccctggtg
     2341 tgattggttc cgtcgcgcct catgcgttaa ccgaaaagca aaagcaacaa ccgctgtcat
     2401 ttgatgagat gttcattgat attggcgcga acagtcgcga agaggtggaa aaacgcggtg
     2461 tggcgatggg cgattttatt agcccggaag ccaattttgc ctgctggggc gaagataaag
     2521 tagtcggcaa ggcgctggat aatcgcatcg gctgcgcgat gatggcggaa ctactacaga
     2581 cagtaaataa ccccgaaatt acgctgtatg gcgttggcag tgtggaagaa gaagttgggc
     2641 tacgcggggc acaaacctcg gcggaacaca ttaaaccgga tgtggtgatc gtgctggata
     2701 ctgctgtcgc aggtgatgtt ccgggcattg ataacatcca atacccgctg aaactgggcg
     2761 acgggccggg gctgatgctg tttgataaac gctacttccc caaccagaaa ctggtggcgg
     2821 cgttaaaaag ctgcgccacg cagaatgatt taccgctgca attttccacg atgaaaaccg
     2881 gtgcgacgga tggcgggcgc tacaacgtga tgggcggcgg acgtccggta gtcgcattgt
     2941 gtctgccaac ccgctatttg catgccaata gcggcatgat ttcaaagacc gattacgatg
     3001 ctctgttaat actgatacgg gattttctga cgaccttaac cgcggagaag gtcaacgcgt
     3061 tcagccagtt ccgtcaggtg agctaatgct aaacgaacgt cagttaaaga ttgtcgatct
     3121 gctggagcaa cagccgcaca cgccaggtga gctggcgcaa caaacgggcg tttccggcag
     3181 gaccattctg cgtgacattg actatctcaa ctttaccctc aacggcaaag cccgcatttc
     3241 tgccggtggc ggtgtgggct atcaactgga gatattcgac cgccgcagct tttttcagtt
     3301 gctgcaaaaa cacgacaatg atgatcggct actggcgctt ttatttctaa atacctttac
     3361 cccgcgcgca caacttgcct cggcacttaa tttgccggaa acctgggtag cagagcgcct
     3421 gctccggcta aagcaacgtt atgaacgcat ttgttgcctg gccagtcgcc cgggtttggg
     3481 tcatttcatt gatgagtgcg aagagaaacg cgttattctg ctggcgaact tgctacgcaa
     3541 agatccgttt ttgcttccac tggctggcgt gacgcgggag agttttcagc aattatccac
     3601 tgcctgcgac aaccaacacc gctggccgct catgcagggt gattatctct ccagcctgat
     3661 tctggctatt tacgccctgc gtaatcaact gaccaacgag tggccggaat atcccagtaa
     3721 tgagataaaa caaatcgttg aacaaagcgg cctgtttctg ggcgataacg ctataagaac
     3781 tctgacgggg ctgatagaaa aacagcatca gcaagcgcaa gcaatttctg ccgataatgt
     3841 actggcgttg ctgcaaagag tgccgggcat cacctcattg aatattatcg atgcgcaatt
     3901 ggtcgagaat attaccgggc atttattacg ctgccttgcc gcgccggtat ggattgctga
     3961 acatcgacaa agcagtatga ataacctgaa agccgcctgg cctgccgcct ttgatatgag
     4021 tctgcatttt attacgctac tgcgcgaaca gttcgacatt cctcttttcg acagcgatct
     4081 gatcggtttg tattttgcct gtgcgctgga acggcatcaa aacgaacgcc agccgatcat
     4141 tttgttatcc gatcagaatg ctattgccac cattaatcag tttgccattg agcgcgatgt
     4201 tttaaattgt cgggtaatta ttgcccgtag tttaggtgag cttgtggcca tttgcgaaga
     4261 gatcgagcca ttattgatca taaataatag ccattattta ctggatgatg cggtcaataa
     4321 ttgtatcacc gtaaaaaaca ttattactgc cgccggtatc gaacaaataa aacattttct
     4381 tgcgacagcg tttattcgcc ggcaaccgga gcgttttttc cctgtcacag gaagtcttca
     4441 ctattcgaat gtgcgaggtg aaagctggca agaaatcact ggccaaattt gtgtgcagct
     4501 cgtggcgcaa cagcatatta ctgccgatga agcgcagcgg atcatcgccc gcgaaagtga
     4561 aggtgaaaac ctgatcgtta atcgcctcgc catccctcat tgctggagcg agcaagagcg
     4621 acgttttcgt ggatttttta ttcccctcgc gcaaccgatt gaggtgaata acgaaatcat
     4681 taaccatgtg ttgatcgcct gcgccgctgc cgatgcgcgt catgagttaa aaatattcag
     4741 ctatcttgcc agcgtgctgt gccagcatcc accggagact attgctgggt taacgggata
     4801 tgaaggattt atagcgttac ttcgccaggg gtgaataatg tcggctccat agctgacgcg
     4861 gcttaatgac gtgtaggtcg gataaggcgt ttacgccgca tccgacattt tatttctcta
     4921 caagatcaaa aacgtttaac gcaggcgatt ataactaccg accagttcgt tataagcgcg
     4981 taatgattgc gggtaagagt ccttaaccat ccagttggtg gttgcgtcct gaagtttctt
     5041 tttatccgag tcagagtacg ggacgttatc tctgatacga cgagcatact ttttagcagc
     5101 aatttgatat cggtctgcag cgccgatgaa gagagaattc atactgtctt tactcacttc
     5161 tttggcttgc gccaccagcg tttcaagttc agccactttc ttcattgccg tttcagcgtc
     5221 aacattctct tgcgaaatca tggcattaat ttgtttcgct aaaatcataa ctgccagaga
     5281 ataatagtgg aatgttttcc cttctctttg ttcaatattt ttcagttcaa caagttggcg
     5341 cttatcatta attacctgaa ttgccgcatg ataagattcc gcaaccggct taaaggcatc
     5401 gacatttttt ataaatacct gatgtagcat ttttcctttc gcaaacgcat cgtctttgta
     5461 attttcctga gtgtaatatt tatccatttc attaatagta ttccccagcg caactgctgc
     5521 atcaatataa ttcaatgcct cgccatcaat gggttccaac gccggcatta acgccactac
     5581 gttttttatt tccttttcac agtcagagag gctggattca ctaatactgt agatgccata
     5641 aacatggagc tctttacccg ttggtccctg tttaaaatct ttcagccagt cagcataacg
     5701 cgccagacta tgctgtacat ggacctgtaa tttgttgtaa cacttgatat agacattcat
     5761 tttttcgtcg aaaaccgctt gttcatcagc caccggttgc gctggcattt ttgcttttgc
     5821 aataggggct tcagtcggtt tcgcttcagg agcgggtgcg gcaggttgac tattggcggg
     5881 cggaggtgtt tctgtttcag cttttttatc atcacaaccc gtgataccga gagccatgat
     5941 ggcgactata atagcggaag ataatagatt ccttttcatt taatttcctt ataaatatat
     6001 agataagaga gtgaacataa taaataaaaa aagaaaaaaa gaaaacgcca ctacacgcgt
     6061 tttgtttata aatgaaataa taaaattcac gaaatgaata agtcctgcaa tttaatatat
     6121 aaaccacagg acttaattca attaatagtt caacctttgc ggtagggaac gttctgtccc
     6181 ccactgttta aacttgcatt acattccata acgctcagaa atagcaacat gcttctcttc
     6241 attcgcaccg gcttctttgg caagacgcca ccagcacaac ggatgtttcc agtcagcggt
     6301 caattcgtgc aaggtcttat cgatatcacc tttagaaact gaacgcggcg cactgataac
     6361 aaagtagaat gcgggcagcg gctgcgcatc ttcaccctga tgactgtccg ttaacgtata
     6421 gtcatcatta ctgttgctcc agtagtacca gtttcgttca cggagcgcct cacggaaggc
     6481 attaaactgt tcttcaccag gcatgacacc ttcgcagaac caggactcgc cgtactcggt
     6541 cgggataatg gttttcagac acccataaag tggcgtgaaa ttcttctgat cttcttcgtc
     6601 ttcaggccgg gtattcaaca tttccgcatc gaaaaccaca atttcatcaa catgttgcgg
     6661 gaaatcggtc cggtaagtcg gctctataat ccttgctgtt accggcccaa tgcgttgagc
     6721 atagtaatag cggtattcgt aattttcccg gaagcgaatg tttagcagcc cccatttttc
     6781 gtcgataaaa ccttcgcctt ctgtgagttc gatttccagt tcagcagcaa tagcacgaac
     6841 taaatcccac tgttgtaaga tgctggcggt cgccatgata tcccactgga gattacgaat
     6901 atcttcttca cggctaatca tcagttgctg caattgctgt aaataatcca gttgtcgatc
     6961 ccaggcttcc tgtttttgtg ctgcccagga acacatgcga taaacgccga cccactcacg
     7021 ccctttttcg ttttccagca actgttgccc aatcgactcc agttcactcc agtcttcctg
     7081 ttggttgatc acccgcgcac ggaaccacag cgccatatgt agcagacccg cctgttcata
     7141 cagcttcgcc agatgctcga tcgcctgctg atcgccacgg ttaagcaaga gatccagtag
     7201 atcgaagcac aggccgttat cttcctgaag gtggctttca acgtagcgtt gcagaagttc
     7261 aatcgactca ttaagcgcat tacaggcgtt taacgcattg accagtttgc ccagcaattc
     7321 cgcattatct ggcgtgttca gataagcgcg acggaatatt tcgacgtctt tttctggatc
     7381 tggctgcgtt tcttcgtcgc tattttcagg tacttctaca accggacgag caagcagttc
     7441 cgcttctttg gcggcaattt tttcactaag ttcgagaaac tcgcgatcga cacccactaa
     7501 cgagcgtaat tttggcatgt gctgctgggc cagtttcatc gccattttgg ccacccagac
     7561 ggattcacgc tccagcgcca gagtgatttg cagttttgcc agatctaccg ctttgcggtg
     7621 tgccccggcc tgcgaacgtt gctccagcat tttttggatg atgcgcgcca gctgccagtg
     7681 gtttctctgc ggatggcgag gaagcagtac ggtaataatc tttacccaca tgccccacga
     7741 atagggagtc agatctgacc aacgcggtaa ttcttcccaa acttcatcgt cctgcccgca
     7801 taacgccatg atgtacagtt ttttgagatg ataattgatg cggtcacggc cccagtaatc
     7861 gttctctttt gccagttcat caatcacttc acgagcttca tcgtagcgct gaagggcaac
     7921 taaaatatcg gcgcgtgttg ccagcagcgc ttctaaatat tcactccccg atgctttcac
     7981 cgcctcaatt tgcctgtcga tataaaccaa cgcttcttct gttttgttgg agtccaccag
     8041 tgcactggca tactcgcagg ataaacacat aaagcagctc caggtcggat caatgcgcgc
     8101 aatggtttct tcacatactg ccatgcgttc attcgcccag cctgggccat cgatattttc
     8161 gtagcaggct gataaatcct gggtaacgca aatggattgc gggcaatctt ttgcctcttc
     8221 ccggtgcgct ttttcaaaca gcgctaccac gtctggcagc accttttcac cttcttcata
     8281 gttagtcagg cgatgacgca tctcccagtg accgataaat atttccagcc acggattttt
     8341 agctacttga ttcagcgctt tcatttccgg caatagcgaa tcgcattttt ctatttgcag
     8401 gtgcaaaaca ctttctgaaa aatcatcaat aagctgcgga atatattctt gcccggcatc
     8461 gcgcaactcc tgactatatt tgttgtacca ctcccatgta tccattttat tttccttgat
     8521 taattaagac aggaataacc tgagtaaaat cttccagtgc ctggtgtaaa tcccactgct
     8581 gttctttatt accgctggag cacaaaatca ctttcagcga ttttaataac cgtaatgctg
     8641 ccgctggctg ctgatggtgt tgtaaggccg tcaccagttg catgacacag gggttattaa
     8701 gattgatata cagacttgat gttttagtct tttggatttg cgaggtaaat tgccgcgcca
     8761 gcattaacgc tgcggtgctg atgcgtttat ctgcgtcatc ctgttcgaga atttgtttta
     8821 attctgcttc gcggtctggc gtaaccacga gcggtaaaac cgctggttcg aagcgagaga
     8881 taattaattg ctcgccgtcg cagagatgct cttccagata agccacctgc tcttcgggta
     8941 attccgccag gctgaacaac tgctcattac ccgtttgcgt accgacttcg ataatccggc
     9001 agtgatataa cagcgcccaa cggcgtaaaa atggcaccac cgcatagcga tcgccacggg
     9061 ctacaggccg ttgcagaata tggaacaaca tctcttcaaa accgccgtcg cggcttaaca
     9121 gaatatgaat gctgttatta acgcgtaaat cctttgccag cagcgcgcct tttgacgttg
     9181 gcacctgcaa gcgatctttg agcaaatcaa acagacggtc atcacacaac gccgcaccga
     9241 gcaacgcttc gttgtggcgc attaatacac gccgccagat ttctggctga ttttgtgcga
     9301 gatcggacaa accagaaatc agcgcctctt ttaacgcccc ctgtaccgcg acccaggttt
     9361 cgtcccgctg gagatcttcc ctgctcgccg tcggcgtcag tttcgatgac tcaatcacac
     9421 caccaataaa accggcccag ggcggcaaca gttcacgcgc ttcgtcatcc agtaacatgc
     9481 cgcgcaaaaa cagtgacagg ttacggttat cgctggtgcc gtaggttgcg ccatcctgaa
     9541 tccataaaat cccgaccgcg tcactcatcc ccgctggcac caccggaata gtgcaaatcg
     9601 gttcgaagga ggattcaaac tgggcggcaa aggcgagatt tttacgctgt accagcgcac
     9661 ggtgcatagt tacgccttcg ggggcaactt cacgccacgg tggttgaagt ttatttaccg
     9721 gctcgctggc atcgccgaca aagaccggtt cgtgcagcaa tatgcagtag cgggaaagaa
     9781 cgcggttcag caaattgtta ctcgccagat ggctgtactc ttctttgagc gtcagaataa
     9841 cctgcgtccc cgtccccgaa gcctgatgtg gcgtaacggt atatttttgc ccgtcggtag
     9901 agtggtattt ccagctctga tccggcgttt gccaggatgt ggtcaacacc gtgacctctt
     9961 ttgccagtac aaatgccgac aaaaaaccga ggccgaacat accaattaaa ccggtgttgt
    10021 catcctgctg gcgcaacatt cgggtataac ccacacccac tgtagccagg aagccgtgaa
    10081 tttcgctttc tgtcagaccc gcgccggtat cggtaatggt gatagtggac ttcgccacgt
    10141 cagccaccac tcgaatttgg ttatcctttg gcgcatcggg ctgctcaatt ctgcggcgca
    10201 cgatcgcatc gtgtccgttt tgcaccagct cccgtacggc gaccacgggg gtagagtaaa
    10261 ggtgcttgct gagcacctca attaagccgt ttaaattaac ttctgtagaa tatgaactgc
    10321 caggtaactg taatgtactc atttttcaat actcataatc catcacctac tattgaaaat
    10381 acattgctgt ttttacaacc ggtgcaaggt ttgtt
//
LOCUS       DAHHTG010000142        10326 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.143, whole genome shotgun sequence.
ACCESSION   DAHHTG010000142 DAHHTG010000000
VERSION     DAHHTG010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10326)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10326)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10326
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.143"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            complement(<1..744)
                     /gene="intI1"
                     /locus_tag="NBI74_003820"
     CDS             complement(<1..744)
                     /gene="intI1"
                     /locus_tag="NBI74_003820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 integron integrase IntI1"
                     /protein_id="HCD1934095.1"
                     /translation="MKTATAPLPPLRSVKVLDQLRERIRYLHYSLRTEQAYVHWVRAF
                     IRFHGVRHPATLGSSEVEAFLSWLANERKVSVSTHRQALAALLFFYGKVLCTDLPWLQ
                     EIGRPRPSRRLPVVLTPDEVVRILGFLEGEHRLFAQLLYGTGMRISEGLQLRVKDLDF
                     DHGTIIVREGKGSKDRALMLPESLAPSLREQLSRARAWWLKDQAEGRSGVALPDALER
                     KYPRAGHSWLWFWVFAQHTHSTDPRSGVVR"
     gene            902..1375
                     /locus_tag="NBI74_003821"
     CDS             902..1375
                     /locus_tag="NBI74_003821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001389366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrA17"
                     /protein_id="HCD1934096.1"
                     /translation="MKISLISAVSENGVIGSGPDIPWSVKGEQLLFKALTYNQWLLVG
                     RKTFDSMGVLPNRKYAVVSKNGISSSNENVLVFPSIENALKELSKVTDHVYVSGGGQI
                     YNSLIEKADIIHLSTVHVEVEGDIKFPIMPENFNLVFEQFFMSNINYTYQIWKKG"
     gene            1506..2294
                     /locus_tag="NBI74_003822"
     CDS             1506..2294
                     /locus_tag="NBI74_003822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000503573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase AadA5"
                     /protein_id="HCD1934097.1"
                     /translation="MGEFFPAQVFKQLSHARAVIERHLAATLDTIHLFGSAIDGGLKP
                     DSDIDLLVTVSAAPNDSLRQALMLDLLKVSSPPGDGGTWRPLELTVVARSEVVPWRYP
                     ARRELQFGEWLRHDILSGTFEPAVLDHDLAILLTKARQHSLALLGPSAATFFEPVPKE
                     HFSKALFDTIAQWNAESDWKGDERNVVLALARIWYSASTGLIAPKDVAAAWVSERLPA
                     EHRPLICKARAAYLGSEDDDLAMRVEETAAFVRYAKATIERILR"
     gene            2500..2847
                     /locus_tag="NBI74_003823"
     CDS             2500..2847
                     /locus_tag="NBI74_003823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000679427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter QacE delta 1"
                     /protein_id="HCD1934098.1"
                     /translation="MKGWLFLVIAIVGEVIATSALKSSEGFTKLAPSAVVIIGYGIAF
                     YFLSLVLKSIPVGVAYAVWSGLGVVIITAIAWLLHGQKLDAWGFVGMGLIIAAFLLAR
                     SPSWKSLRRPTPW"
     gene            2841..3680
                     /gene="sul1"
                     /locus_tag="NBI74_003824"
     CDS             2841..3680
                     /gene="sul1"
                     /locus_tag="NBI74_003824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000259031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonamide-resistant dihydropteroate synthase
                     Sul1"
                     /protein_id="HCD1934099.1"
                     /translation="MVTVFGILNLTEDSFFDESRRLDPAGAVTAAIEMLRVGSDVVDV
                     GPAASHPDARPVSPADEIRRIAPLLDALSDQMHRVSIDSFQPETQRYALKRGVGYLND
                     IQGFPDPALYPDIAEADCRLVVMHSAQRDGIATRTGHLRPEDALDEIVRFFEARVSAL
                     RRSGVAADRLILDPGMGFFLSPAPETSLHVLSNLQKLKSALGLPLLVSVSRKSFLGAT
                     VGLPVKDLGPASLAAELHAIGNGADYVRTHAPGDLRSAITFSETLAKFRSRDARDRGL
                     DHA"
     gene            3808..4011
                     /locus_tag="NBI74_003825"
     CDS             3808..4011
                     /locus_tag="NBI74_003825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000376616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1934100.1"
                     /translation="MDSEEPPNVRVACSGDIDEVVRLMHDAAAWMSAKGTPAWDVARI
                     DRTFAETFVLRSELLGIASENGK"
     gene            4167..5372
                     /gene="chrA"
                     /locus_tag="NBI74_003826"
     CDS             4167..5372
                     /gene="chrA"
                     /locus_tag="NBI74_003826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004896675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromate efflux transporter"
                     /protein_id="HCD1934101.1"
                     /translation="MTNNPTDDSRPWSVFLIFLRLGLTSFGGPIAHLGYFRAEFVTRR
                     RWLSERSYADLVALCQFLPGPASSQVGIAVGLSRAGYSGALAAWAGFTLPSAIALILF
                     ALGISSYGDYVSQGALHGLKVVAVAVVAQAVWGMARNLCTDGLRVTIMAIATCVVLLV
                     PSAWGQVGVIAIAGIAGRLLFKPAKVVEHDPLPITVSHRAGVLWLSLFFVLLIGLPVL
                     AELMPSQTMAMVDSFYRVGSLVFGGGHVVLPLLQAEVVPSGWVNNESFLAGYGAAQAV
                     PGPLFTFAAFLGASMNTAPSGWIGGIVCLLAIFAPSFLLVVGSMPFWERLRRNTGIQA
                     ALAGINAAVVGLLLAALYQPVWTSAIFQPQDFGLALVALVALMFWKLPPWLVVLGSGA
                     AGWLLSVAL"
     gene            5383..5688
                     /locus_tag="NBI74_003827"
     CDS             5383..5688
                     /locus_tag="NBI74_003827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000130000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCD1934102.1"
                     /translation="MTDKDLYGGLIRLHILHHAAEEPVFGLGIIEELRRHGYEMSAGT
                     VYPMLHGLEKKGYLTSRHERTGRRERRVYDITEQGRTALADAKTKVKELFGELVEGG"
     gene            5732..5797
                     /locus_tag="NBI74_003828"
                     /pseudo
     CDS             5732..5797
                     /locus_tag="NBI74_003828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229646.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
     gene            5915..6679
                     /locus_tag="NBI74_003829"
     CDS             5915..6679
                     /locus_tag="NBI74_003829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001389365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS6100 family transposase"
                     /protein_id="HCD1934103.1"
                     /translation="MTDFKWRHFQGDVILWAVRWYCRYPISYRDLEEMLAERGISVDH
                     TTIYRWVQCYAPEMEKRLRWFWRRGFDPSWRLDETYVKVRGKWTYLYRAVDKRGDTID
                     FYLSPTRSAKAAKRFLGKALRGLKHWEKPATLNTDKAPSYGAAITELKREGKLDRETA
                     HRQVKYLNNVIEADHGKLKILIKPVRGFKSIPTAYATIKGFEVMRALRKGQARPWCLQ
                     PGIRGEVRLVERAFGIGPSALTEAMGMLNHHFAAAA"
     gene            complement(7172..7756)
                     /locus_tag="NBI74_003830"
     CDS             complement(7172..7756)
                     /locus_tag="NBI74_003830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001137892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCD1934104.1"
                     /translation="MPRPKLKSDDEVLEAATVVLKRCGPIEFTLSGVAKEVGLSRAAL
                     IQRFTNRDTLLVRMMERGVEQVRHYLNAIPIGAGPQGLWEFLQVLVRSMNTRNDFSVN
                     YLISWYELQVPELRTLAIQRNRAVVEGIRKRLPPGAPAAAELLLHSVIAGATMQWAVD
                     PDGELADHVLAQIAAILCLMFPEHDDFQLLQAHA"
     gene            complement(7756..8994)
                     /locus_tag="NBI74_003831"
     CDS             complement(7756..8994)
                     /locus_tag="NBI74_003831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCD1934105.1"
                     /translation="MSERRYSPLATLFAATFLFRIGNAVAALALPWFVLSHTKSAAWA
                     GATAASSVIATIIGAWVGGGLVDRFGRAPVALISGVVGGVAMASIPLLDAVGALSNTG
                     LIACVVLGAAFDAPGMAAQDSELPKLGHVAGLSVERVSSLKAVIGNVAILGGPALGGA
                     AIGLLGAAPTLGLTAFCSVLAGLLGAWVLPARAARTMTTTATLSMRAGVAFLWSEPLL
                     RPLFGIVMIFVGIVGANGSVIMPALFVDAGRQVAELGLFSSMMGAGGLLGIAIHASVG
                     ARISAQNWLAVAFCGSAVGSLLLSQLPGVPVLMLLGALVGLLTGSVSPILNAAIYNRT
                     PPELLGRVLGTVSAVMLSASPMVMLAAGAFVDLAGPLPGLVVSAVFAGLVALLSLRLQ
                     FATMAAAATASAPTHTEGEH"
     gene            complement(8991..9896)
                     /locus_tag="NBI74_003832"
     CDS             complement(8991..9896)
                     /locus_tag="NBI74_003832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000219391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mph(A) family macrolide 2'-phosphotransferase"
                     /protein_id="HCD1934106.1"
                     /translation="MTVVTTADTSQLYALAARHGLKLHGPLTVNELGLDYRIVIATVD
                     DGRRWVLRIPRRAEVSAKVEPEARVLAMLKNRLPFAVPDWRVANAELVAYPMLEDSTA
                     MVIQPGSSTPDWVVPQDSEVFAESFATALAALHAVPISAAVDAGMLIRTPTQARQKVA
                     DDVDRVRREFVVNDKRLHRWQRWLDDDSSWPDFSVVVHGDLYVGHVLIDNTERVSGMI
                     DWSEARVDDPAIDMAAHLMVFGEEGLAKLLLTYEAAGGRVWPRLAHHIAERLAFGAVT
                     YALFALDSGNEEYLAAAKAQLAAAE"
     gene            complement(10018..>10326)
                     /locus_tag="NBI74_003833"
     CDS             complement(10018..>10326)
                     /locus_tag="NBI74_003833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS26 family transposase"
                     /protein_id="HCD1934107.1"
                     /translation="RFINTDKAPAYGRALALLKREGRCPSDVEHRQIKYRNNVIECDH
                     GKLKRIIGATLGFKSMKTAYATIKGIEVMRALRKGQASAFYYGDPLGEMRLVSRVFEM
                     "
BASE COUNT         2150 a         2975 c         3027 g         2174 t
ORIGIN      
        1 acgcacgaca ccgctccgtg gatcggtcga atgcgtgtgc tgcgcaaaaa cccagaacca
       61 cagccaggaa tgcccggcgc gcggatactt ccgctcaagg gcgtcgggaa gcgcaacgcc
      121 gctgcggccc tcggcctggt ccttcagcca ccatgcccgt gcacgcgaca gctgctcgcg
      181 caggctgggt gccaagctct cgggtaacat caaggcccga tccttggagc ccttgccctc
      241 ccgcacgatg atcgtgccgt gatcgaaatc cagatccttg acccgcagtt gcaaaccctc
      301 actgatccgc atgcccgttc catacagaag ctgggcgaac aaacgatgct cgccttccag
      361 aaaaccgagg atgcgaacca cttcatccgg ggtcagcacc accggcaagc gccgcgacgg
      421 ccgaggtctt ccgatctcct gaagccaggg cagatccgtg cacagcacct tgccgtagaa
      481 gaacagcaag gccgccaatg cctgacgatg cgtggagacc gaaaccttgc gctcgttcgc
      541 cagccaggac agaaatgcct cgacttcgct gctgcccaag gttgccgggt gacgcacacc
      601 gtggaaacgg atgaaggcac gaacccagtg gacataagcc tgttcggttc gtaaactgta
      661 atgcaagtag cgtatgcgct cacgcaactg gtccagaacc ttgaccgaac gcagcggtgg
      721 taacggcgca gtggcggttt tcatggcttg ttatgactgt ttttttgtac agtctatgcc
      781 tcgggcatcc aagcagcaag cgcgttacgc cgtgggtcga tgtttgatgt tatggagcag
      841 caacgatgtt acgcagcagg gcagtcgccc taaaacaaag ttagccatta agggagttaa
      901 attgaaaata tcattgattt ctgcagtgtc agaaaatggc gtaatcggta gtggtcctga
      961 tatcccgtgg tcagtaaaag gtgagcaact actctttaaa gcgctcacat ataatcaatg
     1021 gctccttgtc ggaagaaaaa catttgactc tatgggtgtt cttccaaatc gcaaatatgc
     1081 agtagtgtca aagaacggaa tttcaagctc aaatgaaaac gtcctagttt ttccttcaat
     1141 agaaaatgct ttgaaagagc tatcaaaagt tacagatcat gtatatgtct ctggcggggg
     1201 tcaaatctat aatagcctta ttgaaaaagc agatataatt catttgtcta ctgttcacgt
     1261 tgaagtcgaa ggtgatatca aattccctat aatgcctgag aatttcaatt tggtttttga
     1321 acagtttttt atgtctaata taaattatac ataccagatt tggaaaaaag gctaacaatg
     1381 cgttgcagca ccagtcgctt cgctccttgg acagctttta agtcgcgtct ttgtggtttt
     1441 gctgcgcaaa agtattccac aaagccgcaa cttaaaagct gccgctgaac ttaacgttag
     1501 gcatcatggg tgaatttttc cctgcacaag ttttcaagca gctgtcccac gctcgcgcgg
     1561 tgatcgagcg ccatctggct gcgacactgg acacaatcca cctgttcgga tctgcgatcg
     1621 atggagggct gaagccggac agcgacatag acttgctcgt gaccgtcagc gccgcaccta
     1681 acgattcgct ccggcaggcg ctaatgctcg atttgctgaa agtctcatca ccgccaggcg
     1741 atggcggaac atggcgaccg ctggagctaa ctgttgtcgc tcgaagcgaa gtagtgcctt
     1801 ggcgctatcc ggcgcggcgt gagcttcagt tcggtgagtg gctccgccac gacatccttt
     1861 ccggaacgtt cgagcctgcc gttctggatc acgatcttgc gattttgctg accaaggcga
     1921 ggcaacacag ccttgcgctt ctaggcccat ccgcagccac gtttttcgag ccggtgccga
     1981 aggagcattt ctccaaggcg cttttcgaca ctattgccca gtggaatgca gagtcggatt
     2041 ggaagggtga cgagcggaac gtcgttcttg ctcttgctcg catttggtac agcgcttcaa
     2101 ctggtctcat tgctcctaag gacgttgctg ccgcatgggt atcggagcgt ttgcctgccg
     2161 agcatcggcc cctcatctgc aaggcacgcg cggcgtacct gggtagcgag gacgacgacc
     2221 tagcaatgcg cgtcgaagag acggccgcgt tcgttcgata tgccaaagca acgattgaga
     2281 gaatcttgcg ttgagcggca tgtgcgaaaa gtgcatcgac ccgcgccgag ggcatctgat
     2341 gcctaactcg gcgttcaagc ggacgggctg cgcccgccgc tcaactatgc gttagatgca
     2401 ctaagcacat aattgctcac agccaaacta tcaggtcaag tctgctttta ttatttttaa
     2461 gcgtgcataa taagccctac acaaattggg agatatatca tgaaaggctg gctttttctt
     2521 gttatcgcaa tagttggcga agtaatcgca acatccgcat taaaatctag cgagggcttt
     2581 actaagcttg ccccttccgc cgttgtcata atcggttatg gcatcgcatt ttattttctt
     2641 tctctggttc tgaaatccat ccctgtcggt gttgcttatg cagtctggtc gggactcggc
     2701 gtcgtcataa ttacagccat tgcctggttg cttcatgggc aaaagcttga tgcgtggggc
     2761 tttgtaggta tggggctcat aattgctgcc tttttgctcg cccgatcccc atcgtggaag
     2821 tcgctgcgga ggccgacgcc atggtgacgg tgttcggcat tctgaatctc accgaggact
     2881 ccttcttcga tgagagccgg cggctagacc ccgccggcgc tgtcaccgcg gcgatcgaaa
     2941 tgctgcgagt cggatcagac gtcgtggatg tcggaccggc cgccagccat ccggacgcga
     3001 ggcctgtatc gccggccgat gagatcagac gtattgcgcc gctcttagac gccctgtccg
     3061 atcagatgca ccgtgtttca atcgacagct tccaaccgga aacccagcgc tatgcgctca
     3121 agcgcggcgt gggctacctg aacgatatcc aaggatttcc tgaccctgcg ctctatcccg
     3181 atattgctga ggcggactgc aggctggtgg ttatgcactc agcgcagcgg gatggcatcg
     3241 ccacccgcac cggtcacctt cgacccgaag acgcgctcga cgagattgtg cggttcttcg
     3301 aggcgcgggt ttccgccttg cgacggagcg gggtcgctgc cgaccggctc atcctcgatc
     3361 cggggatggg atttttcttg agccccgcac cggaaacatc gctgcacgtg ctgtcgaacc
     3421 ttcaaaagct gaagtcggcg ttggggcttc cgctattggt ctcggtgtcg cggaaatcct
     3481 tcttgggcgc caccgttggc cttcctgtaa aggatctggg tccagcgagc cttgcggcgg
     3541 aacttcacgc gatcggcaat ggcgctgact acgtccgcac ccacgcgcct ggagatctgc
     3601 gaagcgcaat caccttctcg gaaaccctcg cgaaatttcg cagtcgcgac gccagagacc
     3661 gagggttaga tcatgcctag cattcacctt ccggccgccc gctagcggac cctggtcagg
     3721 ttccgcgaag gtgggcgcag acatgctggg ctcgtcagga tcaaactgca ctatgaggcg
     3781 gcggttcata ccgcgccagg ggagcgaatg gacagcgagg agcctccgaa cgttcgggtc
     3841 gcctgctcgg gtgatatcga cgaggttgtg cggctgatgc acgacgctgc ggcgtggatg
     3901 tccgccaagg gaacgcccgc ctgggacgtc gcgcggatcg accggacatt cgcggagacc
     3961 ttcgtcctga gatccgagct cctagggatc gcctcagaaa acggaaaata aagcacgcta
     4021 agccgtaagt aagcgtgctc ctgtgaaagc cacagctaaa actgcgtagt acacatagag
     4081 gtattttttt ctagatttat atcgaatttc gttatcgaat ttcgcttatt attgatcaac
     4141 tcgttgcaga tcatgaaggc gaaagtatga cgaataaccc taccgatgac agcagaccat
     4201 ggtcggtctt tcttattttt ctgcggcttg gattgacatc ttttggcggc cccattgcgc
     4261 acttgggcta cttccgcgcc gaatttgtca cacggcggcg ctggctctcc gaacggagct
     4321 atgctgactt ggtcgcgctt tgtcagttct tgccagggcc tgcaagcagc caggtcggca
     4381 tagcggtagg actgtctcgg gctggataca gcggggcgct ggctgcttgg gctggcttca
     4441 cgctgccgtc tgccatagcc ttgatccttt ttgcgctcgg catctccagc tatggcgatt
     4501 acgtctcgca gggcgcgttg catggcttaa aagtggtggc tgtggccgtg gtcgctcaag
     4561 cagtatgggg catggcgcgt aacctatgca cggatgggct gcgagtcacc atcatggcaa
     4621 ttgctacctg cgtcgtttta cttgtgccgt ccgcgtgggg acaggttggc gtgattgcta
     4681 tcgcaggcat cgcaggccgg ttattgttca agccagcgaa agttgttgag catgaccccc
     4741 tacctatcac ggtcagtcac cgggccggcg tgctttggct ctcgctgttc tttgtcttgc
     4801 tgattggcct gccggtgttg gccgaactga tgccaagtca aaccatggca atggtggatt
     4861 ccttctatcg tgtcggatca ctggtgttcg gcggtggtca cgttgtgctg ccattactgc
     4921 aagccgaagt ggtgccctcc ggctgggtca acaatgaatc ctttctcgcg gggtacgggg
     4981 cagctcaagc ggtgcccggc cctttgttca cgttcgccgc gtttcttggt gcctcgatga
     5041 acaccgcccc gtcgggctgg atcggcggca ttgtgtgtct gctggctatc ttcgcgccct
     5101 cgttcttgct ggtcgtcgga tcaatgccat tttgggagcg tttgcgccgc aatacaggca
     5161 tccaagctgc gctggccggg atcaatgccg ctgtagtcgg cttgctgctg gccgcgctgt
     5221 atcagcctgt atggactagc gccatctttc agccgcaaga cttcggcttg gcattagttg
     5281 cccttgtcgc acttatgttc tggaagctcc cgccgtggct ggttgtcctc ggtagcggtg
     5341 cagctgggtg gctgttgagc gtcgctctgt gaggcacaaa aaatgactga caaagacctc
     5401 tacggcggtt tgatccgcct gcacatcctt caccatgcag ccgaggaacc tgtctttggg
     5461 ctggggatca tcgaagagct acgccgacac ggctacgaga tgagcgctgg caccgtgtac
     5521 ccgatgctgc acggcctgga aaaaaaaggc tatctgacct cacgccacga acgcaccggg
     5581 cgacgtgaac ggcgtgttta tgacatcact gaacaaggca gaactgcact tgctgatgcg
     5641 aaaacaaagg tcaaggagct gttcggagag ttggtagaag gtggttgagt ttttgcttct
     5701 atgtcaggtt gagctatacc ctaactggat tgtcattttc agaagacgac tgcaccagtt
     5761 gattgggcgt aatggctgtt gtgcagccag ctcctgacag ttcaatatca gaagtgatct
     5821 gcaccaatct cgactatgct caatactcgt gtgggctctg ttgcaaaaat cgtgaagctt
     5881 gagcatgctt ggcggagatt ggacggacgg aacgatgacg gatttcaagt ggcgccattt
     5941 ccagggtgat gtgatcctgt gggcggtgcg ctggtattgt cgctatccga tcagctatcg
     6001 cgaccttgag gaaatgctgg cggaacgcgg catttcggtc gaccatacga cgatctatcg
     6061 ctgggtccag tgctacgccc cggagatgga gaagcggctg cgctggttct ggcggcgtgg
     6121 ctttgatccg agctggcgcc tggatgaaac ctacgtcaag gtgcggggca agtggaccta
     6181 cctgtaccgg gcagtcgaca agcggggcga cacgatcgat ttctacctgt cgccgacccg
     6241 cagcgccaag gcagcgaagc ggttcctggg caaggccctg cgaggcctga agcactggga
     6301 aaagcctgcc acgctcaata ccgacaaagc gccgagctat ggtgcagcga tcaccgaatt
     6361 gaagcgcgaa ggaaagctgg accgggagac ggcccaccgg caggtgaagt atctcaataa
     6421 cgtgatcgag gccgatcacg gaaagctcaa gatactgatc aagccggtgc gcggtttcaa
     6481 atcgatcccc acggcctatg ccacgatcaa gggattcgaa gtcatgcgag ccctgcgcaa
     6541 aggacaggct cgcccctggt gcctgcagcc cggcatcagg ggcgaggtgc gccttgtgga
     6601 gagagctttt ggcattgggc cctcggcgct gacggaggcc atgggcatgc tcaaccacca
     6661 tttcgcagca gccgcctgat cggcgcagag cgacagccta cctctgactg ccgccaatct
     6721 ttgcaacaga gcccgccgtg ctagtctgct cggtgatggt ggagtgaagc caacccgcaa
     6781 tcgggttatg aatctgcatc gcgattcgca atcagctgtc tcttgagcat gtcgaactcc
     6841 tgcgatgtta tctcgccgtg ctcgtgctgc tacaccaact tgtccaactc atccgccaca
     6901 ccgctcccca ccacctgcga tggccaggtt gtttgctggg cgcattggcg gagtatcgtc
     6961 tcgacccggg acacccacac cggcacaacc ttacgggagt gattcactgt caaagaatcg
     7021 gcccggtgct ctgacgcaag tatcgggatg gtcaccattt gtaagccgta gacctgagtg
     7081 gtgatcaaga cttcgatacc accgaccgta ccggtactaa tcgacgacgg tcgtgttcgt
     7141 cgcctgccgc agggactctg cacacctccg tttacgcatg tgcctggagg agttggaaat
     7201 cgtcgtgttc gggaaacatt aaacacagga tggcagcgat ctgagccagc acatgatcag
     7261 ctagctcacc atccggatcg acggcccact gcatcgtcgc gccagcgatg accgagtgca
     7321 ggagcaactc agctgccgca ggagcacctg ggggcagtcg cttgcggatc ccctccacca
     7381 ccgcgcggtt ccgctggatc gcaagcgtgc gtagctccgg cacctggagc tcgtaccagg
     7441 agatgagata gttcaccgag aagtcgttgc gagtgttcat gctccgaacg agcacctgca
     7501 aaaattccca gagcccttgc ggccctgcgc ctatcggtat cgcattcagg taatgccgca
     7561 cctgctcgac gccgcgctcc atcatcctca ccagcagcgt atcgcggttg gtgaagcgct
     7621 ggattaacgc tgcgcgggag agccccacct cctttgctac tccgctgagc gtgaactcta
     7681 tgggaccgca acgcttcagc actacggtgg cggcctcgag tacctcgtca tcggacttga
     7741 gcttggggcg gggcatcagt gttcaccttc tgtatgggtt ggggcggagg ctgtggctgc
     7801 cgccgccatt gtagcaaatt gaagacggag cgagagtaga gccacgagcc ccgcaaacac
     7861 ggccgataca acgaggccag ggagcggacc agcaaggtcg acaaacgcgc cggccgcaag
     7921 cataaccatg ggcgaggctg acagcatcac cgccgagacc gtgccgagta cccggccgag
     7981 aagttctggc ggcgtgcggt tgtagatggc agcgttgaga atgggagaga ctgagccggt
     8041 cagcagtccc acgagcgcgc ccaacaacat cagcaccggc acgcctggca actgtgaaag
     8101 cagaagcgag cccaccgcag agccacaaaa tgccaccgcc agccagttct gcgctgatat
     8161 ccgggcgccg accgacgcat gaatggcaat gccaaggaga ccaccagccc ccatcattga
     8221 ggagaacagc ccgagctctg ctacttggcg tcctgcatct acaaacagcg caggcatgat
     8281 gacgctgccg ttggcgccaa cgatgcccac gaagatcatc actataccaa agagagggcg
     8341 cagcaggggt tcgctccaga gaaaagcgac gccggcgcgc atggagagag tcgccgtcgt
     8401 ggtcatcgtc cgagcggcac gcgcgggaag cacccacgcg ccgagcagac ctgcaaggac
     8461 ggagcagaac gccgtcagcc cgagcgttgg cgcagcgcca agcaggccga ttgcggcccc
     8521 cccaagggcc gggccaccta gaatcgcgac gttcccgatc accgctttca gtgacgagac
     8581 gcgctcaacg gagagcccgg cgacgtggcc gagtttgggc agctcactgt cctgcgcggc
     8641 cataccgggt gcgtcgaacg cggcaccgag caccacgcaa gcgatcagcc cagtgttcga
     8701 gagggcgcca acggcatcga gcagtgggat gctcgccatg gccacgccgc ccaccacacc
     8761 cgagatcaat gcgacgggcg cgcgcccgaa ccgatcgacg aggccaccac caacccacgc
     8821 gccgatgatg gtcgcgatga cgctgctagc ggccgtggcg cccgcccagg ccgcgctctt
     8881 tgtatgagac aggacgaacc atggaagcgc gagggccgcc accgcgttgc cgatccggaa
     8941 gagaaaggtc gccgcgaaca gcgtcgcgag cgggctatat cgacgttcgc tcattccgct
     9001 gcggcgagct gcgccttcgc cgcagcgagg tactcttcgt tacccgagtc gagggcgaag
     9061 agtgcgtagg tgaccgcccc gaacgcaagg cgctccgcga tgtggtgggc gagccgcggc
     9121 cacacccggc caccggccgc ttcatacgtg aggaggagct tcgcgagccc ctcttcacca
     9181 aagaccataa ggtgcgcggc catgtcgatg gcagggtcat caacgcgggc ctcgctccag
     9241 tcgatcatcc cgctgacgcg ctccgtgttg tcgatgagca catggcccac gtagagatcg
     9301 ccatgcacca ccacggagaa atctggccac gacgaatcgt cgtcgagcca gcgctgccac
     9361 cggtggaggc gcttgtcgtt caccacgaac tcgcgtcgga cgcggtcaac gtcgtcggcc
     9421 accttctgac gggcctgcgt cggtgtacgg atgagcatcc ccgcatccac ggcggcggaa
     9481 atggggacgg catgcagggc ggcgagcgcg gtcgcgaagc tctccgcgaa gacctccgag
     9541 tcctgcggca cgacccagtc gggcgtggac gaaccaggct ggatgaccat cgcagtcgag
     9601 tcttcgagca tgggataggc aacgagctcg gcgttggcca cgcgccagtc cggcaccgcg
     9661 aacggcaggc gattcttgag cattgccagc acccgcgcct ctggttcgac cttcgcgctt
     9721 acctcggctc ggcgcgggat gcgcagcacc caccgacgtc catcgtcgac ggtggcgatc
     9781 acgatcctat agtcgagccc aagctcattg acagtcagcg ggccatggag cttgagccca
     9841 tgtcgggctg caagtgcgta cagttgggag gtatcggcgg tcgtgactac ggtcatgatt
     9901 cactcctgag ggcttgacgg gtttagccac ctaaatgtaa cagtcacgtc ggttatattc
     9961 aatccggcac tgttgcaaag ttagcgatga ggcagccttt tgtcttattc aaaggcctta
    10021 catttcaaaa actctgctta ccaggcgcat ttcgcccagg ggatcaccat aataaaatgc
    10081 tgaggcctgg cctttgcgta gtgcacgcat cacctcaata cctttgatgg tggcgtaagc
    10141 cgtcttcatg gatttaaatc ccagcgtggc gccgattatc cgtttcagtt tgccatgatc
    10201 gcattcaatc acgttgttcc ggtacttaat ctgtcggtgt tcaacgtcag acgggcaccg
    10261 gccttcgcgt ttgagcagag caagcgcgcg accataggcg ggcgctttat ccgtgttgat
    10321 gaatcg
//
LOCUS       DAHHTG010000143        10182 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.144, whole genome shotgun sequence.
ACCESSION   DAHHTG010000143 DAHHTG010000000
VERSION     DAHHTG010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10182)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10182)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10182
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.144"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            61..1458
                     /gene="ulaA"
                     /locus_tag="NBI74_003834"
     CDS             61..1458
                     /gene="ulaA"
                     /locus_tag="NBI74_003834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418614.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HCD1934108.1"
                     /translation="MEILYNIFTVFFNQVMTNAPLLLGIVTCLGYILLRKSVSVIIKG
                     TIKTIIGFMLLQAGSGILTSTFKPVVAKMSEVYGINGAISDTYASMMATIERMGDAYS
                     WVGYAVLLALALNICYVLLRRITGIRTIMLTGHIMFQQAGLIAVTLFIFGYSMWTTII
                     CTAILVSLYWGITSNMMYKPTQEVTDGCGFSIGHQQQFASWIAYKVAPFLGKKEESVE
                     DLKLPGWLNIFHDNIVSTAIVMTIFFGAILLSFGIDTVQAMAGKVNWTVYILQTGFSF
                     AVAIFIITQGVRMFVAELSEAFNGISQRLIPGAVLAIDCAAIYSFAPNAVVWGFMWGT
                     IGQLIAVGILVACGSSILIIPGFIPMFFSNATIGVFANHFGGWRAALKICLVMGMIEI
                     FGCVWAVKLTGMSAWMGMADWSILAPPMMQGFFSIGIAFMAVIIVIALAYMFFAGRAL
                     RAEEDAEKQLAEQSA"
     gene            1474..1779
                     /gene="ulaB"
                     /locus_tag="NBI74_003835"
     CDS             1474..1779
                     /gene="ulaB"
                     /locus_tag="NBI74_003835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIB"
                     /protein_id="HCD1934109.1"
                     /translation="MTVRILAVCGNGQGSSMIMKMKVDQFLTQSNIDHTVNSCAVGEY
                     KSELSGADIIIASTHIAGEITVTGNKYVVGVRNMLSPADFGPKLLEVIKEHFPQDVK"
     gene            1789..2253
                     /gene="ulaC"
                     /locus_tag="NBI74_003836"
     CDS             1789..2253
                     /gene="ulaC"
                     /locus_tag="NBI74_003836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIA"
                     /protein_id="HCD1934110.1"
                     /translation="MKLRDSLAENKSIRLQAEAETWQDAVKIGVDLLVAADVVEPRYY
                     QAILDAVEQHGPYFVLAPGLAMPHGRPEEGVKKTGFALVTLKKPLEFNHEDNDPVDIL
                     ITMAAVDANTHQEVGIMQIVNLFEDEENFDRLRACRTEQEVLDLIDRTNAAA"
     gene            2267..2917
                     /gene="ulaD"
                     /locus_tag="NBI74_003837"
     CDS             2267..2917
                     /gene="ulaD"
                     /locus_tag="NBI74_003837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HCD1934111.1"
                     /translation="MSLPMLQVALDNQTMDSAYETTRLIAEEVDIIEVGTILCVGEGV
                     RAVRDLKALYPHKIVLADAKIADAGKILSRMCFEANADWVTVICCADINTAKGALDVA
                     KEFNGDVQIELTGYWTWEQAQQWRDAGIGQVVYHRSRDAQAAGVAWGEADITAIKRLS
                     DMGFKVTVTGGLALEDLPLFKGIPIHVFIAGRSIRDAASPVEAARQFKRSIAELWG"
     gene            2927..3781
                     /gene="ulaE"
                     /locus_tag="NBI74_003838"
     CDS             2927..3781
                     /gene="ulaE"
                     /locus_tag="NBI74_003838"
                     /EC_number="5.1.3.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase UlaE"
                     /protein_id="HCD1934112.1"
                     /translation="MLSKQIPLGIYEKALPAGECWLERLQLAKTLGFDFVEMSVDETD
                     DRLSRLDWSREQRLALVNAIVETGVRVPSMCLSAHRRFPLGSEDDAVRAQGLEIMRKA
                     IQFAQDVGIRVIQLAGYDVYYQEANNETRRRFRDGLKESVEMASRAQVTLAMEIMDYP
                     LMNSISKALGYAHYLNNPWFQLYPDIGNLSAWDNDVQMELQAGIGHIVAVHVKDTKPG
                     VFKNVPFGEGVVDFERCFETLKQSGYCGPYLIEMWSETAEDPAAEVAKARDWVKARMT
                     KAGMVEAV"
     gene            3781..4467
                     /gene="ulaF"
                     /locus_tag="NBI74_003839"
     CDS             3781..4467
                     /gene="ulaF"
                     /locus_tag="NBI74_003839"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase UlaF"
                     /protein_id="HCD1934113.1"
                     /translation="MQKLKQQVFEANMDLPRYGLVTFTWGNVSAIDRERGLVVIKPSG
                     VAYETMKADDMVVVDMSGKVVEGEYRPSSDTATHLELYRRYPSLGGIVHTHSTHATAW
                     AQAGLAIPALGTTHADYFFGDIPCTRGLSEEEVQGEYELNTGKVIIETLGDAEPLHTP
                     GIVVYQHGPFAWGKDAHDAVHNAVVMEEVAKMAWIARSINPQLNHIDSFLMNKHFMRK
                     HGPNAYYGQK"
     gene            complement(4596..4871)
                     /locus_tag="NBI74_003840"
     CDS             complement(4596..4871)
                     /locus_tag="NBI74_003840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HCD1934114.1"
                     /translation="MFSRVLALLAVLLLSANTWAAIEINNHQARNMDDVQSLGVIYIN
                     HNFATESEARQALNEETDAQGATYYHVILMREPGSNGNMHASADIYR"
     gene            5199..5594
                     /gene="rpsF"
                     /locus_tag="NBI74_003841"
     CDS             5199..5594
                     /gene="rpsF"
                     /locus_tag="NBI74_003841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HCD1934115.1"
                     /translation="MRHYEIVFMVHPDQSEQVPGMIERYTAAITGAEGKIHRLEDWGR
                     RQLAYPINKLHKAHYVLMNVEAPQEVIDELETTFRFNDAVIRSMVMRTKHAVTEASPM
                     VKAKDERRERRDDFANETADDAEAGDSEE"
     gene            5601..5915
                     /gene="priB"
                     /locus_tag="NBI74_003842"
     CDS             5601..5915
                     /gene="priB"
                     /locus_tag="NBI74_003842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N"
                     /protein_id="HCD1934116.1"
                     /translation="MTNRLVLSGTVCRTPLRKVSPSGIPHCQFVLEHRSVQEEAGFHR
                     QAWCQMPVIVSGHENQAITHSITVGSRITVQGFISCHKAKNGLSKMVLHAEQIELIDS
                     GD"
     gene            5920..6147
                     /gene="rpsR"
                     /locus_tag="NBI74_003843"
     CDS             5920..6147
                     /gene="rpsR"
                     /locus_tag="NBI74_003843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012986941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HCD1934117.1"
                     /translation="MARYFRRRKFCRFTAEGVQEIDYKDIATLKNYITESGKIVPSRI
                     TGTRAKYQRQLARAIKRARYLSLLPYTDRHQ"
     gene            6189..6638
                     /gene="rplI"
                     /locus_tag="NBI74_003844"
     CDS             6189..6638
                     /gene="rplI"
                     /locus_tag="NBI74_003844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HCD1934118.1"
                     /translation="MQVILLDKVANLGSLGDQVNVKAGYARNFLVPQGKAVPATKKNI
                     EFFEARRAELEAKLAEVLAAANARAEKINALETVTIASKAGDEGKLFGSIGTRDIADA
                     VTAAGVEVAKSEVRLPNGVLRTTGEHEVSFQVHSEVFAKVIVNVVAE"
     gene            7040..7531
                     /locus_tag="NBI74_003845"
     CDS             7040..7531
                     /locus_tag="NBI74_003845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000516891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCD1934119.1"
                     /translation="MGKKTAPLLPYTNQMLSEFGERLKLARLRRRLTAKQVAERAGMS
                     PITLRSLESGSAGVTIGAYLSVMQVLGLETDLNKLAAEDDLGRQLQDSRLIKKRGLSR
                     VSSKNEEGSQTASLQMRHEKEGHLTTDLTALAEGSTPSITGETSTSLAALLKPIKKPL
                     KGN"
     gene            7534..8801
                     /locus_tag="NBI74_003846"
                     /pseudo
     CDS             7534..8801
                     /locus_tag="NBI74_003846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001606689.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HipA domain-containing protein"
     gene            complement(8944..>10182)
                     /locus_tag="NBI74_003847"
     CDS             complement(8944..>10182)
                     /locus_tag="NBI74_003847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCD1934120.1"
                     /translation="LVCFITVINYLDRTALGIAAPTIMETTGITKEQYSWIVSAFQLA
                     YTLGQPIMGFFIDTVGLKLSFAICAAIWGLATMGHALTGTWSGLAFMRALMGFSEASA
                     IPAGVKTASTWFPAKERGVATGVFNMGTSLGAMLAPPLIAWCIMFHSWQFAFIVSGSL
                     ALLAALFWFFCYKDPKDAKRLSDEERHYIESGQEQHLKTDKKEKTSIKHILSQRNFWG
                     IGIARFLADPAWGTINFWVPIFFVETLHFSLKEIAMFVWLPFLLGDLGCLASGFVAKF
                     FHDRGVSLINSRRITFTIAAVIMMTIGLVSIVENPYIAVLLISIGAFSHQCLSTVAAT
                     LGGDLFKKDEVATAVGMAGACAWSGQLIFNLFIGAFVHIIGFAPFFIALAFFDIIGAI
                     ALWTLIKVKDEEPQVQLATS"
BASE COUNT         2597 a         2379 c         2682 g         2524 t
ORIGIN      
        1 tgagagcaca acattccggg tgtgcgaaat acccggttac ctcttcttca ggagatcgtt
       61 atggagatcc tctacaacat ctttaccgtg ttttttaacc aggtcatgac caatgccccg
      121 ttgttgctgg gtattgtgac ctgtctgggt tacatcctac tgcgcaaaag tgtcagcgtt
      181 attattaaag gtacgattaa aaccataatt ggtttcatgt tgttgcaggc agggtccggc
      241 atcctgacca gcaccttcaa accggtggtg gcgaaaatgt ccgaagtcta cggcattaac
      301 ggcgcaattt ccgatactta cgcgtcgatg atggcaacca ttgaacgcat gggcgatgcc
      361 tatagctggg tgggttacgc ggtgttgtta gcgctggcgc tgaacatctg ttacgtgctg
      421 ctgcgtcgca ttaccggtat tcgtaccatc atgctgactg gtcacatcat gttccagcag
      481 gcggggctga ttgccgttac gctgtttatc ttcggctact ccatgtggac gaccattatc
      541 tgcaccgcga ttctggtttc gctctactgg ggcattacct ccaacatgat gtacaagccg
      601 acacaggaag tcaccgacgg ctgtggtttc tccattggtc accagcagca gttcgcatca
      661 tggattgctt ataaagtcgc accgttcctc ggcaaaaaag aggagagcgt tgaagacctc
      721 aaattgccgg gttggctgaa cattttccac gacaacatcg tctccacggc gattgtcatg
      781 accatcttct ttggtgccat tctgctctcc tttggtatcg acaccgtgca ggcgatggca
      841 ggcaaagtga actggacggt gtacatcctg caaaccggtt tctcctttgc ggtggcgatc
      901 ttcatcatca ctcagggcgt gcgcatgttt gtggcggaac tctctgaagc atttaacggt
      961 atctcccagc gtctgatccc aggtgcggtt ctggcgattg actgtgcggc tatttatagc
     1021 ttcgcgccga acgccgtggt ctggggtttt atgtggggca ccatcgggca actgattgcg
     1081 gttggcatcc tggtcgcctg tggctcttca atcctgatta ttcctggctt tatcccgatg
     1141 ttcttctcca acgccactat cggcgtgttc gctaaccact tcggcggctg gcgtgcggcg
     1201 ctgaaaattt gtctggtgat ggggatgatc gaaatctttg gttgcgtctg ggcagtgaaa
     1261 ctcaccggta tgagtgcctg gatgggtatg gcggactggt cgattctggc accgccgatg
     1321 atgcagggct tcttctccat cggtatcgcc tttatggccg tcatcattgt aattgcactg
     1381 gcttatatgt tcttcgctgg ccgcgcgctg cgcgcagaag aagatgcaga aaaacaactg
     1441 gcagaacaat ctgcttaata aggagttttg attatgaccg tacgtattct ggctgtgtgt
     1501 ggtaacggac aaggcagttc catgatcatg aaaatgaaag tggaccagtt tttaacccag
     1561 tcgaacattg accacacggt aaacagctgc gcggttggcg aatacaaaag cgagttgagc
     1621 ggcgcggata tcatcatcgc ttctacgcac attgcgggcg aaatcaccgt gaccggcaac
     1681 aaatatgtgg ttggcgtgcg caacatgctc tctcctgctg attttggccc gaaactgctg
     1741 gaagtgatca aagagcattt cccgcaggac gtgaagtaag gacgcgccat gaaattacgt
     1801 gattcgctgg ctgaaaataa atctatccgc ctgcaagcgg aagcagagac atggcaggac
     1861 gcagtgaaaa tcggcgttga tctgctggta gcggcagatg tggtagagcc gcgttactac
     1921 caggcgattc tggatgcggt tgaacagcac ggcccgtact ttgtgctcgc ccccggcctg
     1981 gcaatgccgc acggacgccc ggaagagggc gttaagaaaa ccggttttgc gctggtgacg
     2041 ctgaaaaagc cgctggagtt caaccacgaa gataacgatc cggtggatat cctcatcacc
     2101 atggcggcgg ttgatgccaa cactcaccag gaagtgggca tcatgcagat cgtcaacctg
     2161 tttgaagatg aagagaattt cgaccgttta cgtgcctgcc gtaccgagca ggaagtactg
     2221 gatctcattg accgcaccaa cgcggcagct taagaaggaa ttgaacatgt cattaccgat
     2281 gttgcaagtc gcgctggaca accagactat ggatagcgcc tacgaaacca ctcgcctgat
     2341 tgccgaagaa gtcgacatta tcgaagtagg caccattctt tgcgtaggcg aaggcgtgcg
     2401 tgcggttcgt gacctgaaag cgctgtaccc gcacaaaatt gtgctggcag acgccaaaat
     2461 tgccgatgcg ggcaaaatcc tttctcgcat gtgcttcgaa gccaacgctg actgggtgac
     2521 cgtaatttgt tgtgcggata tcaacaccgc caaaggcgcg ctggacgtgg caaaagagtt
     2581 taatggcgac gtgcagatcg aactgaccgg ctactggacc tgggaacagg cgcagcagtg
     2641 gcgcgacgca ggcattgggc aggtggttta tcaccgcagc cgtgacgcgc aggccgcagg
     2701 cgtggcgtgg ggcgaagcgg acattaccgc gatcaaacgt ctttccgata tgggcttcaa
     2761 agtcaccgtc accggtggtc tggcgctgga agatctgccg ttgtttaaag gcattccgat
     2821 tcatgtgttt atcgctggtc gcagcatccg tgatgcagcc tctccggttg aagccgcgcg
     2881 tcagttcaaa cgttccatcg ccgaactgtg gggctaagga gcggatatgt tgtccaaaca
     2941 aatcccgctt ggcatctacg aaaaagcgct ccccgctggg gagtgctggc tggaacgact
     3001 gcaactggca aaaacgttag gcttcgattt tgtcgagatg tcagtagatg aaacggacga
     3061 tcgcctttcg cgcctcgact ggagccgcga gcagcgtctg gcactggtga acgcgattgt
     3121 tgaaaccggc gtgcgcgtac cgtccatgtg cctttctgct catcgtcgtt tcccgctggg
     3181 aagcgaagat gacgcggtgc gggcgcaggg gctggagatt atgcgtaaag ctatccagtt
     3241 cgcccaggac gtcggcattc gcgtgatcca actggcgggc tatgacgttt actatcagga
     3301 agccaataac gaaacgcgtc gtcgttttcg tgacggcctg aaagagagcg ttgagatggc
     3361 aagccgcgcg caggtcacgc tggcgatgga gatcatggat tacccattga tgaactccat
     3421 cagcaaggcg ctgggctacg cacactatct caacaatccg tggttccagc tctacccgga
     3481 tatcggcaac ctgtcggcgt gggacaacga cgtgcagatg gagttgcagg ccggaatcgg
     3541 gcatatcgtc gcggtacatg tgaaagacac caaaccaggc gtcttcaaaa acgtgccttt
     3601 tggcgaaggt gttgtggatt ttgaacgttg cttcgaaacg ctcaaacaga gtggctattg
     3661 cgggccgtac ctgattgaga tgtggagcga aacggcggaa gatccggcgg cagaagtggc
     3721 gaaagcgcgc gattgggtga aagcgcgcat gaccaaagca ggcatggtgg aggcggtata
     3781 atgcaaaagc tgaaacagca ggtgtttgag gccaacatgg atctgccgcg ttacgggctg
     3841 gtgaccttta cctggggcaa cgtcagcgcc attgaccgcg aacgcgggct ggtggtgatc
     3901 aagcccagcg gcgtcgccta cgaaaccatg aaagcagacg atatggtggt ggtcgatatg
     3961 agcggtaagg tggtggaagg ggagtatcgt ccgtcttccg acactgcgac gcatctcgaa
     4021 ctttaccgtc gttatccgtc gcttggtggt attgtccata cccactccac tcatgccacc
     4081 gcatgggcgc aggcggggct ggcgatcccg gcgttaggca ccacgcacgc cgattacttc
     4141 tttggcgaca tcccgtgcac gcgcggatta agcgaagaag aggtgcaggg tgagtacgaa
     4201 ctgaataccg gcaaagtgat tatcgaaacg ctgggtgacg ccgagccgct gcatacgccg
     4261 ggaattgtgg tgtatcagca cggaccgttc gcctggggga aagatgccca cgatgcggtg
     4321 cataacgcgg tggtgatgga agaagtggcg aaaatggcgt ggattgcgcg cagtattaac
     4381 ccacaactga atcacatcga cagctttctg atgaataaac acttcatgcg taagcacggt
     4441 cccaacgcct attacgggca gaagtaaaac acgcgctgcg ggaatttcct tcttcgggag
     4501 ataactggtc taattccgca gccgtttttc aaaaaaagcc ccctgcgaag ggggcaaagc
     4561 aaactatggc aatgtttcgt tggttatacc tggtgctagc gataaatatc cgcgctggcg
     4621 tgcatattgc cgttactccc cggttcccgc atcagaatca catggtagta cgttgcgcct
     4681 tgcgcatctg tctcttcatt taatgcctga cgtgcttcac tttcagtggc gaagttatgg
     4741 ttgatataaa tcacacctaa gctttgcaca tcatccatat ttctggcctg gtggttatta
     4801 atttcaatgg ctgcccatgt atttgcactt agcaaaagca cagccagaag ggctaaaaca
     4861 cgactgaaca tagatacctc ctcgacggct gactttgtgt gctctcctct tgtggtgatc
     4921 ttctgattta attttaatca atgataaaga agttgatggt gaccatttct gatgcagttg
     4981 ttcaaaaaaa caccatgacg aagtgtgatg aactccaaat cagcgattta gaggttattt
     5041 gcgaaagggg cgatttattt ctgctcagcc cttgagttta gcggggtatg tcagtactat
     5101 aacgcgtcat ttttcagccg acctttaaca cgttccttgc ctccccggga ttcggctgac
     5161 ccagacagga ggctgaataa tccgtaagga gcaattcgat gcgtcattac gaaatcgttt
     5221 ttatggtcca tcctgaccag agcgaacagg ttccgggcat gatcgagcgc tacactgctg
     5281 ccatcactgg tgcagaaggc aagatccacc gtctggaaga ctggggccgc cgtcagctgg
     5341 cttacccgat caacaaactg cacaaagcac actacgttct gatgaacgtt gaagctccgc
     5401 aggaagtgat cgatgagctg gaaactacct tccgcttcaa cgatgccgtt atccgcagca
     5461 tggttatgcg taccaagcac gctgttaccg aagcatctcc gatggttaaa gcgaaagacg
     5521 agcgccgtga gcgtcgcgat gatttcgcaa acgaaaccgc tgatgatgct gaagctgggg
     5581 attctgaaga gtaatttctg atgaccaacc gtctggtgtt gtccggcacc gtgtgcagga
     5641 ctccccttcg aaaggtcagt ccatcaggaa ttcctcactg ccagttcgtg cttgagcatc
     5701 gttctgtgca ggaggaagcc ggctttcacc ggcaggcgtg gtgccaaatg cccgttattg
     5761 ttagcggaca cgaaaaccag gccattactc acagtataac ggtcggcagt cgcataaccg
     5821 ttcaggggtt catttcatgc cataaggcaa agaacggact gagcaaaatg gttttgcatg
     5881 ccgagcagat tgaattgata gattctggag actagccata tggcacgtta tttccgtcgt
     5941 cgcaagttct gccgtttcac cgcggaaggc gttcaagaga tcgactataa agatatcgct
     6001 acgctgaaaa actacatcac cgaaagcggt aagattgtcc caagccgtat caccggtacc
     6061 cgtgcaaaat accagcgtca gctggctcgc gctatcaaac gcgctcgcta cctgtccctg
     6121 ctgccgtaca ctgatcgcca tcagtaatcg gtcacggtcc attaatacga ctttgagagg
     6181 ataaggtaat gcaagttatt ctgcttgata aagtagcaaa cctgggtagc ctgggtgatc
     6241 aggtaaacgt taaagcgggc tatgctcgta acttcctggt accgcagggt aaagctgttc
     6301 cagctaccaa gaaaaacatt gaattcttcg aagcacgtcg cgctgaactg gaagctaaac
     6361 tggctgaagt tctggcagca gctaatgctc gcgctgagaa aatcaatgca ctggaaactg
     6421 ttaccatcgc gtctaaagct ggcgacgaag gtaaactgtt cggttccatc ggtactcgcg
     6481 acatcgctga cgctgtaact gcagctggcg ttgaagtggc taagagcgaa gttcgtctgc
     6541 cgaacggcgt tctgcgtacc actggtgaac acgaagtgag cttccaggtt cacagcgaag
     6601 tattcgcgaa agtgatcgta aacgtagtag ctgaataatt cgttattcaa cgagacgtaa
     6661 aaacgccgac cagtggtcgg cgttttgctt ttaagtgata aaaaccgcag aaacagacgt
     6721 tccctccata aatctcttgc tattcctttt ataaaaagtt cgcagtaccc atacctggcc
     6781 cccagcctcg ccgctggtag ttcgtgtcat gttttttacg cgtaaaacac gacaaaagca
     6841 tcaatacagc gatcgtctca cttgtatgat aataaaaaca atatgttata tatgaatgtg
     6901 ctgttatttt attgttttta tatataaaaa gataaaatat catcactttc gtaatcttga
     6961 acgctttgca ggtgcgttca atccaataaa tgctaatatt atggctatca tagccataaa
     7021 aagataaaat attatcacta tgggtaaaaa aacagcaccg ttactccctt atacgaatca
     7081 gatgcttagt gaatttggtg agcggctgaa acttgctcgt ttacgacgca ggcttaccgc
     7141 aaagcaagtt gctgaacgtg ctgggatgtc gccaataaca ttgcgctctc tggagtcagg
     7201 aagtgcaggg gtcacgatag gggcatattt gtctgtgatg caggttttgg gcctggaaac
     7261 ggatttaaat aagcttgcag ctgaagacga cttagggcgt cagctccagg attcccgttt
     7321 gattaaaaaa agagggctgt ctcgtgtatc aagtaagaat gaagaaggtt ctcagacagc
     7381 ctcattgcag atgaggcacg agaaagaagg tcatctcacc acggatttaa cagcactggc
     7441 agaaggctcg acgccatcta ttacaggaga aacatcaacc tctcttgctg cactattaaa
     7501 accgataaaa aaaccgttaa aaggaaatta attatggcat taatcggagt gtatgcggat
     7561 tgggaaggcc tggatggtcc tgcgcgtatt ggttatttac acagtcgtcg cactcgggcg
     7621 cgtgagatat ttgagtttga gtacgataaa aaggcgcttg cagatccttc acttaatttc
     7681 attcagttag atcctgaaat aatgttgtat gagggagcac agtacccaat tcctccaaag
     7741 gataaatttg gtgcatttag tgattcctgc ccggatcgtt gggggcgtat gctaatgaag
     7801 cgtcgttttg agagggatat tcgagacggg ttatgtgata aagactccca cctttatgag
     7861 tctgattatc tactaggtgt tcatgatctt tatcgggttg gggctcttcg atacaaacgt
     7921 gaagacgctg gagagtttct tgataatcgc attgatgttg cagccccacc atttaccgaa
     7981 atagcgagtc tggaaagggt tagccgggca attgaagaag atcctgataa taaagagtaa
     8041 tggggcaaaa atggttaaga atgctaatcg ctccgggggg atcattaggg ggggcacgtc
     8101 cgaaagccag tgttgttgat gaagctgggc atctctacat cgctaaattt cccagtgtaa
     8161 aagatgagta tgatgttggt ggatgggaaa tggtcgttaa tgcacttgct gttggctgtg
     8221 gactgaatgt tgcacctgct caggcgcata aatttgccag caattaccat tgttttatgg
     8281 ttcgtcgttt tgaccgcact aatgcgggaa ggcgtctgca ttttgcttcg gctatgacac
     8341 taaccaggca tcaggatggt gaagatgcct ctacaggtgt gagttatctg gaattagcag
     8401 atgttcttat cagacatggc gcgcagacta atactgacct taaggaactc tggtccagga
     8461 ttgtttttaa tattcttgtt actaataccg atgatcattt acgcaatcat ggatttatct
     8521 tgataccagg aaaaggttgg cggctttctg aggcatatga tttgaatcct gtggtcaggt
     8581 ctgatggtct aaaactcaat attacggaaa atgacaatgc acttgatttg gaattagcta
     8641 gagaagttgc agagtatttt cgacttagtt tgacggaagc tgatgacatt attgctaatt
     8701 ttagagggat tgtcagtcag tggcgaatca ttgccgagcg tcttcgatta cctggtcgtg
     8761 agcaagagtt aatggcagag gcgttcaggg gggcaattta gaatagttat ttcgatatat
     8821 tcgctaaatc ataaatttgc gaagcaatgc cagtagatac tgaacgtttt ggaagagacg
     8881 tttaatttat gagaaaaata gaaacgctac aaagctaaat tatagcgttt ctatgtaaaa
     8941 taattagctt gtcgctaact gtacttgtgg ttcttcgtct ttaactttga taagcgtcca
     9001 cagcgcaatg gcgccaataa tgtcaaagaa agccagggca ataaagaacg gcgcgaagcc
     9061 gataatgtga acgaatgccc cgataaacag gttgaaaatc aactgaccgc tccatgcaca
     9121 ggctcctgcc atacccacag cggtagctac ttcgtctttt ttgaataggt caccgcccag
     9181 agttgctgca acagtagaaa gacattgatg cgagaatgcg ccaatgctaa tcagtaatac
     9241 ggcgatgtag ggattttcga caatactcac cagaccaatc gtcatcataa tgacggctgc
     9301 aatagtgaag gtaattcttc gtgagttaat taaactcacg ccgcgatcgt ggaagaactt
     9361 cgcgacaaaa ccactggcta aacagccgag atcgcccagc aggaaaggta gccagacgaa
     9421 catggcaatt tctttcaggc taaaatgcag cgtttcgacg aagaaaatcg gtacccagaa
     9481 gttaatggtt ccccatgccg ggtcagcgag aaaacgcgcg atgccaatcc cccagaaatt
     9541 acgttggctg aggatatgct tgattgacgt tttttctttc ttatctgttt taagatgctg
     9601 ttcttgccca gactcaatat agtggcgctc ttcatcagaa aggcgtttag catcttttgg
     9661 atctttataa caaaagaacc aaaataaagc cgcgagtaaa gcaaggctac ctgagacaat
     9721 aaacgcaaat tgccagctat gaaacataat gcaccaggca atcaacggtg gagcaagcat
     9781 cgcgccgagt gaggtgccca tattgaaaac acctgtcgcc acgccacgct cttttgccgg
     9841 gaaccatgtt gatgcggttt ttacacccgc aggaattgcc gatgcttcgc tgaaacccat
     9901 cagggcgcgc ataaatgcca gacctgacca tgttcctgtc aaggcatgcc ccattgtcgc
     9961 caggccccag attgcggcac atatcgcaaa acttaacttc agacccacgg tatcaataaa
    10021 gaagcccatt atcggttgcc ccaatgtata ggccaactgg aatgcactga caatccatga
    10081 atattgttct ttggttattc cggttgtctc cataatcgtt ggagccgcaa tacctaacgc
    10141 cgttctgtcc agataattga ttacagtgat aaagcatacg ag
//
LOCUS       DAHHTG010000144        10148 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.145, whole genome shotgun sequence.
ACCESSION   DAHHTG010000144 DAHHTG010000000
VERSION     DAHHTG010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10148)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10148)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10148
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.145"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            137..604
                     /gene="slyB"
                     /locus_tag="NBI74_003848"
     CDS             137..604
                     /gene="slyB"
                     /locus_tag="NBI74_003848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein SlyB"
                     /protein_id="HCD1934121.1"
                     /translation="MIKRVLVVSMVGLSLVGCVNNDTLSGDVYTASEAKQVQNVSYGT
                     IVNVRQVQIQGGDDSNVIGAIGGAVLGGFLGNTIGGGTGRSLATAAGAVAGGVAGQGV
                     QSAMNKTQGVELEIRKDDGNTIMVVQKQGNTHFSPGQRVVLASNGSQVTVSPR"
     gene            complement(651..1085)
                     /gene="slyA"
                     /locus_tag="NBI74_003849"
     CDS             complement(651..1085)
                     /gene="slyA"
                     /locus_tag="NBI74_003849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416159.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SlyA"
                     /protein_id="HCD1934122.1"
                     /translation="MESPLGSDLARLVRIWRALIDHRLKPLELTQTHWVTLHNIHQLP
                     PDQSQIQLAKAIGIEQPSLVRTLDQLEEKGLISRQTCASDRRAKRIKLTEKAEPLISE
                     MEAVINKTRAEILHGISAEELEQLIKLIAKLEHNIIELQAKG"
     gene            1286..1522
                     /locus_tag="NBI74_003850"
     CDS             1286..1522
                     /locus_tag="NBI74_003850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1656 domain-containing protein"
                     /protein_id="HCD1934123.1"
                     /translation="MKFMLNATGLPLQDLVFGASVYFPPFFKAFAFGFVIWLVIHRLL
                     RGWIYAGDIWHPLLMDLSLFAICVCLALAILIAW"
     gene            1525..2382
                     /locus_tag="NBI74_003851"
     CDS             1525..2382
                     /locus_tag="NBI74_003851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416161.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HCD1934124.1"
                     /translation="MSIKTIKYFSTIIVAVVAVLAGWWLWNYYMQSPWTRDGKIRAEQ
                     VSITPQVSGRIVELNIKDNQLVNAGDLLLTIDKTPFQIAELNAQAQLAKAQSDLAKAN
                     NEANRRRHLSQNFISAEELDTANLNVKAMQASVDAAQATLKQAQWQLAQTEIRAPVSG
                     WVTNLTTRIGDYADTGKPLFALVDSHSFYVIGYFEETKLRHIREGAPAQITLYSDNKT
                     LQGHVSSIGRAIYDQSVESDSSLIPDVKPNVPWVRLAQRVPVRFTLDKVPGDVTLVSG
                     TTCSIAVGQ"
     gene            2382..4394
                     /locus_tag="NBI74_003852"
     CDS             2382..4394
                     /locus_tag="NBI74_003852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HCD1934125.1"
                     /translation="MNASSWSLRNLPWFRATLAQWRYALRNTIAMCLALTVAYYLNLD
                     EPYWAMTSAAVVSFPTVGGVISKSLGRIAGSLLGAIAALLLAGHTLNEPWFFLLSMSA
                     WLGFCTWACAHFTNNVAYAFQLAGYTAAIIAFPMVNITEASQLWDIAQARVCEVIVGI
                     LCGGMMMMILPSSSDATALLTALKNMHARLLEHASLLWRPETTDAIRAAHEGVIGQIL
                     TMNLLRIQAFWSHYRFRQQNARLNALLHQQLRMTSVISSLRRMLLNWPSPPDATREIL
                     EQLLTALASSQTDVYTVARIISPLRPTNVTDYRHIAFWQRLRYFCRLYLQSSQELHRL
                     QSGVDDHARLPRTSGLARHTDNAEAMWSGLRTFCTLMMIGAWSIASQWDAGANALTLA
                     AISCVLYSAVAAPFKSLSLLMRTLVLLSLFSFVVKFGLMVQISDLWQFLLFLFPLLAT
                     MQLLKLQMPKFAALWGQLIVFMGSFIAVTNPPVYDFADFLNDNLAKIVGVALAWLAFA
                     ILRPGSDARKSRRHIRALRRDFVDQLSRHPTLSESEFESLTYHHVSQLSNSQDALARR
                     WLLRWGVVLLNCSHVVWQLRDWESRSDPLSRVRDNCISLLRGVMSERGVQQKSLAATL
                     EELQRICDSLARHHQPAARELAAIVWRLYCSLSQLEQAPPQGTLAS"
     gene            complement(4395..4916)
                     /gene="sodC"
                     /locus_tag="NBI74_003853"
     CDS             complement(4395..4916)
                     /gene="sodC"
                     /locus_tag="NBI74_003853"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Cu-Zn] SodC2"
                     /protein_id="HCD1934126.1"
                     /translation="MKRFSLAILALVVATGAQAASEKVEMNLVTSQGVGQSIGSVTIT
                     ETDKGLEFSPDLKALPPGEHGFHIHAKGSCQPATKDGKASAAESAGGHLDPQNTGKHE
                     GPEGAGHLGDLPALVVNNDGKATDAVIAPRLKSLDEVKDKALIVHVGGDNMSDQPKPL
                     GGGGERYACGVIK"
     gene            complement(4997..5893)
                     /locus_tag="NBI74_003854"
     CDS             complement(4997..5893)
                     /locus_tag="NBI74_003854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HCD1934127.1"
                     /translation="MVQRITIAPQGPEFSHFVMGYWRLMDWNMSARQLVSFIEEHLDL
                     GVTTVDHADIYGGYQCEAAFGEALKLAPHLRERMEIVSKCGIATTACEENVIGHYITD
                     RDHIIKSAEQSLINLATDHLDLLLIHRPDPLMDADEVADAFKHLHQSGKVRHFGVSNF
                     TPAQFALLQSRLPFTLATNQVEISPVHQPLLLDGTLDQLQQLRVRPMAWSCLGGGRLF
                     NDDYFQPLRDELAVVAEELNAGSIEQVVYAWVLRLPSQPLPIIGSGKIERVRAAVEAE
                     TLKMTRQQWFRIRKAALGYDVP"
     gene            complement(5942..6181)
                     /locus_tag="NBI74_003855"
     CDS             complement(5942..6181)
                     /locus_tag="NBI74_003855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1289 domain-containing protein"
                     /protein_id="HCD1934128.1"
                     /translation="MTEQLEFFPVQSPCRGICQSDERGFCRGCFRSRDERFNWNKMSD
                     GEKQEVLRLCRQRLMRKLRANKPASSDEPEQPSLF"
     gene            6284..6883
                     /gene="nemR"
                     /locus_tag="NBI74_003856"
     CDS             6284..6883
                     /gene="nemR"
                     /locus_tag="NBI74_003856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416166.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NemR"
                     /protein_id="HCD1934129.1"
                     /translation="MNKHTEHDTREHLLATGEQLCLQRGFTGMGLSELLKTAEVPKGS
                     FYHYFRSKEAFGVAMLERHYAAYHQRLTELLQSGEGNYRDRILAYYQQTLNQFCQHGT
                     ISGCLTVKLSAEVCDLSEDMRSAMDKGARGVIALLSQALENGRENHCLTFCGEPLQQA
                     QVLYALWLGANLQAKISRSFEPLENALAHVKNIIATPAV"
     gene            6920..8017
                     /gene="nemA"
                     /locus_tag="NBI74_003857"
     CDS             6920..8017
                     /gene="nemA"
                     /locus_tag="NBI74_003857"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-ethylmaleimide reductase"
                     /protein_id="HCD1934130.1"
                     /translation="MSSEKLYSPLKVGAITAANRIFMAPLTRLRSIEPGDIPTPLMAE
                     YYRQRASAGLIISEATQISAQAKGYAGAPGIHSPEQIAAWKKITAGVHAENGHMAVQL
                     WHTGRISHASLQPGGQAPVAPSALSAGTRTSLRDENGQAIRVETSMPRALELEEIPGI
                     VNDFRQAIANAREAGFDLVELHSAHGYLLHQFLSPSSNHRTDQYGGSVENRARLVLEV
                     VDAGIEEWGADRIGIRISPIGTFQNTDNGPNEEADALYLIEQLGKRGIAYLHMSEPDW
                     AGGEPYTDAFREKVRARFHGPIIGAGAYTVEKAETLIGKGLIDAVAFGRDWIANPDLV
                     ARLQRKAELNPQRAESFYGGGAEGYTDYPTL"
     gene            8098..8505
                     /gene="gloA"
                     /locus_tag="NBI74_003858"
     CDS             8098..8505
                     /gene="gloA"
                     /locus_tag="NBI74_003858"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HCD1934131.1"
                     /translation="MRLLHTMLRVGDLQRSIDFYTNVLGMKLLRTSENPEYKYSLAFV
                     GYGPETEEAVIELTYNWGVDKYELGTAYGHIALSVDNAAEACEKIRQNGGNVTREAGP
                     VKGGTTVIAFVEDPDGYKIELIEEKDAGRGLGN"
     gene            8608..9255
                     /gene="rnt"
                     /locus_tag="NBI74_003859"
     CDS             8608..9255
                     /gene="rnt"
                     /locus_tag="NBI74_003859"
                     /EC_number="3.1.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease T"
                     /protein_id="HCD1934132.1"
                     /translation="MSDNAQLTGLCDRFRGFYPVVIDVETAGFNAKTDALLEIAAITL
                     KMDEQGWLMPDTTLHFHVEPFVGANLQPEALAFNGIDPNDPDRGAVSEYEALHEIFKV
                     VRKGIKASGCNRAIMVAHNANFDHSFMMAAAERASLKRNPFHPFATFDTAALAGLALG
                     QTVLSKACQTAGMDFDSTQAHSALYDTERTAVLFCEIVNRWKRLGGWPLPAAAEV"
     gene            9484..9576
                     /locus_tag="NBI74_003860"
     CDS             9484..9576
                     /locus_tag="NBI74_003860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001441976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HCD1934133.1"
                     /translation="MNDLPVRQTPMFTLLREAGFSSSPQGLDWG"
     gene            complement(9627..9974)
                     /gene="grxD"
                     /locus_tag="NBI74_003861"
     CDS             complement(9627..9974)
                     /gene="grxD"
                     /locus_tag="NBI74_003861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monothiol glutaredoxin 4"
                     /protein_id="HCD1934134.1"
                     /translation="MSTTIEKIQRQIAENPILLYMKGSPKLPSCGFSAQAVQALAACG
                     ERFAYVDILQNPDIRAELPKYANWPTFPQLWVDGELVGGCDIVIEMYQRGELQQLIKE
                     TAAKYKSEEPDAE"
BASE COUNT         2367 a         2617 c         2557 g         2607 t
ORIGIN      
        1 tataattggt tgatcctact ttcattatga tttgctcata tttggtagaa catgtaacca
       61 tggattcaca tatgccatat actttgacca tgagggatgc ttgcgtggcg tttcatggtg
      121 aacaggagat ttttcaatga ttaaacgcgt attggttgtt tcaatggtag gtctgtctct
      181 tgtcggttgt gttaataacg acaccctgtc aggggatgtt tataccgctt ctgaagcgaa
      241 acaagtacag aatgtcagct atggcaccat cgttaatgta cgtcaggtac agattcaggg
      301 cggtgatgat tccaacgtta tcggcgcaat tggcggtgct gtacttggtg gtttcctggg
      361 aaatactatt ggtggcggaa ccgggcgttc tctggctact gcagcagggg ccgttgcagg
      421 tggtgtagcc ggtcagggcg tacagagtgc aatgaacaaa acgcagggtg tcgagctgga
      481 aattcgtaaa gatgatggca acaccatcat ggtggtacag aaacaaggga acactcattt
      541 ctctccaggc caacgtgtcg tactggccag caatggcagt caggtgaccg tttccccgcg
      601 ctaaataagt ttgcgtgtgg tcaggttact gaccacacgc ccccttcatt tcaccctttg
      661 gcctgtaact caatgatatt atgctcaagt tttgcgatga gcttaatcag ttgttccagt
      721 tcctctgcgg agatgccatg taaaatttcc gcgcgggttt tgttaataac agcctccatt
      781 tcgctgatca gtggctctgc cttttccgtc agtttaatac gtttagcccg acgatcgctg
      841 gcacaagttt gacgcgaaat taaccctttt tcttccagtt ggtccagcgt acggaccagt
      901 gatggctgct cgatgccaat cgcttttgcc agttgaattt gcgactggtc tggaggtaac
      961 tgatggatat tgtgtaacgt aacccaatgg gtttgtgtta actccagcgg tttcaggcga
     1021 tggtctatca gagcacgcca tatgcgcacc aaccgtgcca gatcagaacc tagtggcgat
     1081 tccaatttca tctccttata attagcttgc taagatatta tgcggctttt agaatagtgt
     1141 gcagcaattg tattgctaaa acaaatgtat tgctgcattt ggttaccgtc agacatattt
     1201 ttcagaaatt gcgcgtaaat ttttcgcact taaaaaatat ttattaatct aacgcaatat
     1261 attctgtcgt aaaggaatct actttgtgaa gtttatgctc aatgcaacag gattgccctt
     1321 acaagatctg gtgttcggtg cgtccgtcta ctttcctccg tttttcaaag ccttcgcgtt
     1381 tggattcgtc atctggcttg tcatacaccg cctgcttcgt ggctggatct acgctggtga
     1441 catctggcat cccttgttaa tggatttatc gctgtttgcg atttgcgttt gccttgctct
     1501 ggcaatactg attgcgtggt aactatgtca attaaaacaa ttaagtattt ctcaacaatc
     1561 attgtagcgg tagttgcggt tcttgccgga tggtggctgt ggaattatta catgcaatct
     1621 ccgtggacac gcgacgggaa aatacgcgca gaacaggttt ctatcacccc acaggtgtcg
     1681 ggacgtattg ttgagctgaa tataaaagac aaccagctgg ttaacgcggg ggatcttctg
     1741 ctcacgatcg ataaaacgcc ctttcagatc gccgagctga acgcacaggc tcagctggcg
     1801 aaagcgcaat ctgacctcgc aaaagccaat aacgaagcga atcgtcgtcg tcatctctca
     1861 caaaatttta tttctgccga agaactcgat accgctaacc tcaatgttaa agcgatgcag
     1921 gccagcgttg atgccgcaca ggcgacgctg aaacaggcac aatggcaact ggcgcaaacg
     1981 gaaattcgcg ccccggtgag tggatgggtg actaatctca ctacccgcat cggcgactac
     2041 gctgacaccg ggaaaccgct gtttgccctt gttgacagcc actcgtttta cgtcattggt
     2101 tattttgaag aaaccaagtt gcgccatatc cgcgaaggtg caccagcaca aattaccctc
     2161 tatagtgata acaaaacgtt acagggtcac gtgtccagta tcggtcgggc gatttatgat
     2221 cagagcgttg aaagtgactc aagccttatt ccggatgtga aacctaacgt cccctgggtt
     2281 cgcctcgccc aacgcgttcc cgtccgcttt acgctggata aagtcccagg cgatgtcacg
     2341 ctggtgtcgg gcaccacctg tagcattgcc gtaggtcaat aatgaacgca tcgtcatggt
     2401 ccttgcgcaa tttgccctgg ttcagggcca cgctggcgca atggcgttat gcgttacgca
     2461 ataccattgc catgtgtctg gcgctgacgg ttgcctatta tttgaatctg gatgaaccct
     2521 attgggcgat gacctcggct gcggtggtta gctttcccac cgttggcggt gtcatcagca
     2581 aaagcctcgg acgcatcgct ggcagtttgc tcggagccat tgcagcactg cttcttgccg
     2641 gacatacgct caatgagccg tggttttttc tattgagcat gtcggcgtgg cttggctttt
     2701 gtacctgggc ctgtgcgcac ttcacgaata acgtcgcgta tgcatttcaa ctggcgggct
     2761 acacggctgc catcatcgcc tttccgatgg ttaatattac tgaggccagc cagctgtggg
     2821 atatcgctca ggcgcgtgtt tgcgaggtga tagtcggtat tttgtgcggc ggcatgatga
     2881 tgatgatcct gccgagcagt tccgatgcta cagccctttt aaccgcattg aaaaacatgc
     2941 acgcccgatt actggaacat gccagtttac tctggcggcc tgaaacaacc gatgccattc
     3001 gtgcagcaca tgaaggggtg attgggcaga tactgaccat gaatttgctg cgtatccagg
     3061 ctttctggag ccactatcgt tttcgccagc aaaacgcacg ccttaatgcg ctgctccacc
     3121 agcaattacg tatgaccagt gtcatctcca gcctgcgacg tatgttgctc aactggccct
     3181 caccgccaga tgccacgcga gaaattctcg aacagctgct gacggcgctc gccagttcgc
     3241 aaacagatgt ttacaccgtc gcacgtatta tctccccact acgcccgacc aacgtcaccg
     3301 actaccggca catcgccttc tggcagcgac tacgttattt ttgccgcctt tatctgcaaa
     3361 gtagtcagga attacatcgt ctacaaagcg gtgtagatga tcatgccaga ctcccacgga
     3421 catccggcct ggctcgtcat accgataacg ccgaagctat gtggagcggg ctgcgtacat
     3481 tttgtacgtt gatgatgatt ggcgcatgga gtattgcttc gcaatgggat gccggtgcca
     3541 atgctttaac gctggcagca attagctgcg tactctactc cgccgtcgca gcaccgttta
     3601 agtctttgtc actgctgatg cgcacgctgg tgttactttc gctattcagc tttgtggtca
     3661 aatttggcct gatggtgcag attagcgatc tgtggcaatt tttactgttt ctctttccac
     3721 tgctggcgac aatgcagctt cttaaattgc agatgcccaa atttgccgca ctgtgggggc
     3781 aactgattgt ttttatgggt tcgtttatcg ctgtcactaa tcccccggtg tatgattttg
     3841 ctgattttct taacgataat ctggcaaaaa ttgttggcgt cgcgttggcg tggttagcgt
     3901 tcgccattct gcgtccagga tcggatgctc gtaaaagccg ccgccatatt cgcgcgctgc
     3961 gccgggattt tgtcgatcaa ctaagccgcc atccaacact gagtgaaagc gaatttgaat
     4021 cgctcactta tcatcacgtc agtcagttga gtaacagcca ggatgcgctg gctcgccgtt
     4081 ggttattacg ctggggtgta gtgttgctga actgttctca tgttgtctgg caattgcgcg
     4141 actgggaatc gcgttccgat ccgttatcgc gagtacggga taactgtatt tcactgttgc
     4201 ggggagtgat gagtgagcgt ggcgttcagc aaaaatcact ggcggccaca cttgaagaat
     4261 tacagcggat ttgcgacagc cttgcccgtc atcatcaacc tgccgcccga gaactggcgg
     4321 caattgtctg gcggctgtac tgctcgcttt cgcaacttga gcaagcaccg ccgcaaggta
     4381 cgctggcctc ttaattactt aattacacca caggcatagc gttcaccgcc accgcccagc
     4441 ggtttgggtt gatcggacat attatcgccg ccaacgtgga ctatcagcgc tttgtctttg
     4501 acttcatcca gtgatttcag acgaggcgcg atgacggcat cggtcgcctt gccgtcatta
     4561 ttgactacca gtgcaggcag atcgcctaaa tgcccggcac cttctggccc ttcatgttta
     4621 ccggtatttt gtggatcaag atgcccgcct gcggattctg cggcgctggc tttgccatct
     4681 ttggtggctg gctggcagct tcctttggca tgaatatgga agccatgttc accgggaggt
     4741 aatgctttca gatcgggcga aaactccaga cctttatcgg tttcagtaat ggtgacgcta
     4801 ccaattgact gcccaacccc ttgcgacgtg acgaggttca tctcgacttt ttcactggca
     4861 gcttgtgcgc cggtcgcaac aaccagcgcc agaatggcca gactaaaacg tttcatagga
     4921 cctccgttca ttgtgcattc ctgaataagt gtaaaccagt gacacatttt tgaacggcgg
     4981 ctattcctaa aagtgcttac ggtacgtcgt aacctagtgc cgctttacgg atacggaacc
     5041 attgttgacg ggtcattttc agtgtttctg cttcgactgc tgcccgtacg cgctcaattt
     5101 tacctgagcc gataattggc agcggctgcg acggtaaacg taatacccag gcgtaaacca
     5161 cctgttcaat cgagcccgcg tttaactcct ctgccaccac agccagttca tcacgcagcg
     5221 gctggaaata atcatcatta aacagacgac caccaccaag gcaggaccac gccatcggac
     5281 gaacacgcag ttgttgcagt tggtcgagcg tgccatccag cagtaacggc tgatgcaccg
     5341 gggatatttc cacctgatta gtggcaaggg taaatggcag gcgtgattgc aacagcgcaa
     5401 attgcgcagg tgtaaagttc gatacaccaa aatgacgcac tttgccgctc tgatgcagat
     5461 gtttaaaggc gtccgccact tcatcggcat ccattaacgg gtctggtcgg tggattaaca
     5521 gcagatccag atgatcggtc gcgagattaa ttagcgactg ttcggcgctc ttaatgatgt
     5581 gatcgcggtc agtgatgtaa tggccaatga cgttttcttc acacgcggtc gtcgcgatac
     5641 cgcatttact gacgatttcc atccgttcac gcaggtgagg tgccagtttc agcgcctcgc
     5701 caaacgccgc ttcgcactga tagccaccat aaatatcagc atggtccacg gtggtcacgc
     5761 cgagatccag atgctcttca ataaaactga ccaactggcg ggcggacatg ttccagtcca
     5821 tcaatcgcca gtagcccatc acaaaatggg aaaactccgg gccttgcggc gcaatagtaa
     5881 tacgctgaac cataatcgct tcctcttatc agatatgaga ggagtatacg caagattagg
     5941 ctcaaaagag tgatggttgc tccggttcgt ctgatgacgc tggcttattt gcacgtaatt
     6001 tgcgcattaa tcgctgccga caaaggcgca acacttcttg tttttcgcca tcgctcattt
     6061 tattccagtt aaaacgctca tcccggctac gaaaacagcc gcgacaaaat ccccgttcgt
     6121 cagactggca aattccccgg cacgggctct ggacgggaaa gaactctaat tgctccgtca
     6181 cctcgccctc ctcagataag attattacca ttattgaagc tgttaatgtc caaagtagca
     6241 actttgcttg cactagaccg actggtctac tacactccaa cgcatgaaca aacacaccga
     6301 acatgatact cgcgaacatc tcctggcgac gggcgagcaa ctttgcctgc aacgtggatt
     6361 caccgggatg gggctaagcg aactcctaaa aaccgctgaa gtgccgaaag ggtccttcta
     6421 tcactacttt cgttctaaag aagcgtttgg cgttgccatg cttgagcgcc attacgccgc
     6481 atatcaccag cgactgactg agttgctgca atccggcgaa ggtaactacc gcgaccgcat
     6541 actggcttat taccagcaaa cactgaacca gttttgccaa catggaacca tcagtggttg
     6601 cctgacagta aaactctctg ccgaagtgtg cgatctgtca gaagatatgc gcagcgcaat
     6661 ggataaaggg gctcgcggcg tgatcgccct gctctctcag gcgctggaaa atggccgtga
     6721 gaaccattgt ttaacctttt gtggcgaacc gctgcaacag gcacaagtgc tttacgcact
     6781 gtggcttggt gcgaatttgc aggccaaaat ttcgcgcagt ttcgagccac tggaaaacgc
     6841 gctggcccat gtaaaaaaca ttattgcgac gcctgccgtt tagcaggcat tttttatcac
     6901 cagacgaccg ggagccttta tgtcatctga aaaactgtat tccccactga aagtgggcgc
     6961 gatcacggcg gcaaaccgta tttttatggc accgctgacg cgtctgcgca gtattgaacc
     7021 gggtgacatc cctaccccgt tgatggcgga atactatcgc caacgtgcca gtgccggttt
     7081 gattattagc gaagccacac aaatttctgc ccaggcaaaa ggatacgcag gtgcacctgg
     7141 catccatagc ccggaacaaa ttgccgcatg gaaaaaaatt accgctggcg ttcatgctga
     7201 aaatggtcat atggccgtac agctgtggca caccggacgc atttctcacg ccagcctgca
     7261 acctggcggt caggcaccgg tagcgccttc agcacttagc gcgggaacac gtacttctct
     7321 gcgcgatgaa aatggtcagg cgatccgtgt tgaaacatcc atgccgcgtg cgcttgaact
     7381 ggaagagatt ccgggtatcg tcaatgattt ccgtcaggcc attgctaacg cgcgtgaagc
     7441 cggttttgat ctggtagagc tccactctgc tcacggttat ttgctgcatc agttcctttc
     7501 cccttcttca aaccatcgta ccgatcagta cggcggcagc gtggaaaatc gcgcgcgtct
     7561 ggtgctggaa gtggtcgatg ccgggattga agaatggggt gccgatcgca ttggcattcg
     7621 catttcgcca atcggtactt tccagaacac ggataacggc ccgaatgaag aagccgatgc
     7681 actgtatctg attgaacaac tgggtaaacg cggcattgct tatcttcata tgtcagaacc
     7741 agattgggcg ggaggtgaac catatactga tgcgtttcgc gaaaaagtac gcgcccgttt
     7801 ccacggtccg attatcggcg caggtgcata cacagtagaa aaagctgaaa cgctgatcgg
     7861 caaagggtta attgatgcgg tagcatttgg tcgtgactgg attgcgaacc cggatctggt
     7921 cgcccgcttg cagcgcaaag ctgagcttaa cccgcagcgt gccgaaagtt tttatggtgg
     7981 cggtgctgaa ggttacactg attacccgac gctgtaatcc aacattgcga gcggcgcaaa
     8041 gccgccgcta tactaaaaca actttttgaa tctgttagcc attttgagga tataaagatg
     8101 cgtcttcttc ataccatgct gcgcgttggc gatctgcaac gctccatcga tttttatacc
     8161 aacgtgctgg gcatgaaact gctgcgtacc agcgaaaacc cggaatacaa atactcactg
     8221 gcgtttgttg gttacggccc ggaaaccgaa gaagcagtga ttgaactgac ctacaactgg
     8281 ggcgtggata aatacgaact cggcactgct tatggtcaca tcgcgcttag cgtagataac
     8341 gccgctgaag cgtgcgaaaa aatccgtcaa aacgggggta acgtaacccg tgaagcgggt
     8401 ccggtaaaag gcggtactac ggttatcgca tttgtggaag atccggacgg ttacaaaatt
     8461 gaattaatcg aagagaaaga cgccggtcgc ggtctgggca actaatctcc tgccgggcgt
     8521 gaactcatcg cgcccgcatc tttactgcat cgacaagtaa tatttgtcat aatgcgcgct
     8581 gcaatttatc cgtattaaga gaatcagatg tccgataacg ctcaacttac cggtctgtgc
     8641 gaccgttttc gtggttttta tcctgttgtg atcgatgttg aaacagccgg atttaacgcc
     8701 aaaaccgatg cgctgcttga gattgccgcc atcaccctga aaatggatga acaaggctgg
     8761 ctgatgccgg acaccacatt acatttccac gtcgaaccat ttgtcggcgc aaatttgcaa
     8821 ccagaggccc ttgccttcaa tggcattgat ccgaacgatc ccgatcgcgg cgcggtcagc
     8881 gagtacgagg cgctgcacga aatttttaaa gttgtacgta aaggtattaa agcgagcggc
     8941 tgtaaccgcg ccattatggt ggcgcacaac gccaatttcg atcacagctt tatgatggcc
     9001 gccgcagaac gcgcctcact gaaacgtaac ccgttccacc ctttcgccac ttttgacact
     9061 gctgcactgg ccgggctggc actcggacaa accgtattgt caaaggcttg ccagacagct
     9121 ggcatggact tcgacagcac ccaggcgcac tccgcgctgt acgacaccga acgcactgct
     9181 gtgctgtttt gtgaaatcgt caaccgctgg aaacgtctgg gaggctggcc actacctgcc
     9241 gccgcagagg tgtaatcgag tcgatgcctg atgacatgca atgattcagg catctatagt
     9301 gaggctattc cacgcatcat gcatgatatt cacggggaat agcgttaatg gcaaataatc
     9361 caaacccttc atcgcccctg ccggacgtgt tttcgccggc gacccgcgac tggtttcttc
     9421 gcgcctttaa acagccgacc gctgtctagt cgcgaaccac ggctgcgctt tacgctaaca
     9481 gaagtgaatg acctaccggt ccggcaaacg ccgatgttta cgctgttacg tgaggcggga
     9541 ttttcaagtt cgccacaagg gctggattgg ggatagagaa aggactgacg gatgcccgtt
     9601 cgcatccgtc agtattgcag gacggattat tccgcgtccg gctcttcaga cttgtattta
     9661 gcggcagttt ctttgatcag ctgctgcagt tcaccacgct gatacatttc gatcacgata
     9721 tcacaaccgc ccaccagctc gccgtcaacc cacagttgcg ggaaggtcgg ccagttcgca
     9781 tatttcggca gttccgcacg aatgtccgga ttctgcagaa tatcaacata ggcaaaacgt
     9841 tcgccacatg cggcaagcgc ctggactgcc tgggcagaga aaccgcagct cggcagtttc
     9901 ggtgaacctt tcatgtacag caggatcggg ttttcagcaa tctggcgttg gattttttcg
     9961 atagtggtgc tcattgtctt gcttcctcaa acttctttac ggcagtaatc tgacattgta
    10021 gcgggtcagt gcggcatcgg aaaataacat tttcatcacg cttttgctat tttatccctt
    10081 tgctctattt gttgcatttc aaatattcgt ttcttccctg atgcacggca aactggcgta
    10141 tttataac
//
LOCUS       DAHHTG010000145        10114 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.146, whole genome shotgun sequence.
ACCESSION   DAHHTG010000145 DAHHTG010000000
VERSION     DAHHTG010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10114)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10114)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10114
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.146"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            141..356
                     /gene="bdm"
                     /locus_tag="NBI74_003862"
     CDS             141..356
                     /gene="bdm"
                     /locus_tag="NBI74_003862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm-dependent modulation protein"
                     /protein_id="HCD1934135.1"
                     /translation="MFTYYQAENSTAEPALVNAIEQGLRAEHGVVTEDDILMELTKWV
                     EASDNDILSDIYQQTINYVVSGQHPTL"
     gene            458..595
                     /gene="sra"
                     /locus_tag="NBI74_003863"
     CDS             458..595
                     /gene="sra"
                     /locus_tag="NBI74_003863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary-phase-induced ribosome-associated
                     protein"
                     /protein_id="HCD1934136.1"
                     /translation="MKSNRQARHILGLDHKISNQRKIVTEGDKSSVVNNPTGRKRPAE
                     K"
     gene            751..2448
                     /gene="maeA"
                     /locus_tag="NBI74_003864"
     CDS             751..2448
                     /gene="maeA"
                     /locus_tag="NBI74_003864"
                     /EC_number="1.1.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HCD1934137.1"
                     /translation="MEPKTKKQRSLYIPYAGPVLLEFPLLNKGSAFSMEERRNFNLLG
                     LLPEVVETIEEQAERAWIQYQGFKTEIDKHIYLRNIQDTNETLFYRLVNNHLDEMMPV
                     IYTPTVGAACERFSEIYRRSRGVFISYQNRHNMDDILQNVPNHNIKVIVVTDGERILG
                     LGDQGIGGMGIPIGKLSLYTACGGISPAYTLPVVLDVGTNNQQLLNDPLYMGWRNPRI
                     TDDEYYEFVDEFIQAVKQRWPDVLLQFEDFAQKNAMPLLNRYRNEICSFNDDIQGTAA
                     VTVGTLIAASRAAGGQLSEKKIVFLGAGSAGCGIAEMIIAQTQREGLSEEAARQKVFM
                     VDRFGLLTDKMPNLLPFQTKLVQKRENLSDWDTDSDVLSLLDVVRNVKPDILIGVSGQ
                     TGLFTEEIIREMHKHCPRPIVMPLSNPTSRVEATPQDIIAWTEGNALVATGSPFNPVV
                     WKDKIYPIAQCNNAFIFPGIGLGVIASGASRITDEMLMSASETLAQYSPLVLNGEGLV
                     LPELKDIQKVSRAIAFAVGKMAQQQGVAVKTSAEALQQAIDDNFWHAEYRDYRRTSI"
     gene            2582..3592
                     /gene="adhP"
                     /locus_tag="NBI74_003865"
     CDS             2582..3592
                     /gene="adhP"
                     /locus_tag="NBI74_003865"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
                     /protein_id="HCD1934138.1"
                     /translation="MKAAVVTKDHHVDVTDKTLRSLKHGEALLKMECCGVCHTDLHVK
                     NGDFGDKTGVILGHEGIGVVAEVGPGVTSLKPGDRASVAWFYEGCGHCEYCNSGNETL
                     CRSVKNAGYSVDGGMAEECIVVADYAVKVPDGLDSAAASSITCAGVTTYKAVKLSKIR
                     PGQWIAIYGLGGLGNLALQYAKNVFNAKVIAIDVNDEQLKLATKMGADLAINSRTEDA
                     AKIVQEKAGGAHAAVVTAVAKAAFNSAVDAVRAGGRVVAVGLPPESMSLDIPRLVLDG
                     IEVVGSLVGTRQDLTEAFQFAAEGKVVPKVALRPLEDINTIFTEMEEGKIRGRMVIDF
                     RR"
     gene            3778..4055
                     /locus_tag="NBI74_003866"
                     /pseudo
     CDS             3778..4055
                     /locus_tag="NBI74_003866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011039853.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            4055..4339
                     /locus_tag="NBI74_003867"
     CDS             4055..4339
                     /locus_tag="NBI74_003867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415994.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antidote protein"
                     /protein_id="HCD1934139.1"
                     /translation="MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTG
                     KAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAEKTVDVSRLRRLVTQ"
     gene            complement(4389..5042)
                     /gene="fdnI"
                     /locus_tag="NBI74_003868"
     CDS             complement(4389..5042)
                     /gene="fdnI"
                     /locus_tag="NBI74_003868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit gamma"
                     /protein_id="HCD1934140.1"
                     /translation="MSKSKMIVRTKFIDRACHWTVVICFFLVALSGISFFFPTLQWLT
                     QTFGTPQMGRILHPFFGIAIFVALMFMFVRFVHHNIPDKKDIPWLLNIVEVLKGNEHK
                     VADVGKYNAGQKMMFWSIMSMIFVLLVTGVIIWRPYFAQYFPIQVVRYSLLIHAAAGI
                     ILIHAILIHMYMAFWVKGSIKGMIEGKVSRRWAKKHHPRWYREIEKAEAKKESEEGI"
     gene            complement(5035..5919)
                     /gene="fdnH"
                     /locus_tag="NBI74_003869"
     CDS             complement(5035..5919)
                     /gene="fdnH"
                     /locus_tag="NBI74_003869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase N subunit beta"
                     /protein_id="HCD1934141.1"
                     /translation="MAMETQDIIKRSATNSITPPSQVRDYKAEVAKLIDVSTCIGCKA
                     CQVACSEWNDIRDEVGHCVGVYDNPADLSAKSWTVMRFSETEQNGKLEWLIRKDGCMH
                     CEDPGCLKACPSAGAIIQYANGIVDFQSENCIGCGYCIAGCPFNIPRLNKEDNRVYKC
                     TLCVDRVSVGQEPACVKTCPTGAIHFGTKKEMLELAEQRVAKLKARGYEHAGVYNPEG
                     VGGTHVMYVLHHADQPELYHGLPKDPKIDTSVSLWKGALKPLAAAGFIATFAGLIFHY
                     IGIGPNKEVDDEEEGNHE"
     gene            complement(5932..8979)
                     /gene="fdnG"
                     /locus_tag="NBI74_003870"
     CDS             complement(5932..8979)
                     /gene="fdnG"
                     /locus_tag="NBI74_003870"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(8392..8394),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HCD1934142.1"
                     /translation="MDVSRRQFFKICAGGMAGTTVAALGFAPKQALAQARNYKLLRAK
                     EIRNTCTYCSVGCGLLMYSLGDGAKNAREAIYHIEGDPDHPVSRGALCPKGAGLLDYV
                     NSENRLRYPEYRAPGSDKWQRISWEEAFSRIAKLMKADRDANFIEKNEQGVTVNRWLS
                     TGMLCASGASNETGMLTQKFARSLGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANVVMVMGGNAAEAHPVGFRWAMEAKNNNDATLIVVDPRFTRTASVADIYAPIR
                     SGTDITFLSGVLRYLIENNKINAEYVKHYTNASLLVRDDFAFEDGLFSGYDAEKRQYD
                     KSSWNYQFDENGYAKRDDTLTHPRCVWNLLKAHVSRYTPDVVENICGTPKADFLKVCE
                     VLASTSAPDRTTTFLYALGWTQHTVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSTSLPGYLTLPSEKQVDLQSYLEANTPKATLADQVNYWSNYPKFFVSL
                     MKSFYGDAAQKENNWGYDWLPKWDQTYDVIKYFNMMDEGKVTGYFCQGFNPVASFPDK
                     NKVVSCLSKLKYMVVIDPLVTETSTFWQNHGESNDVDPASIQTEVFRLPSTCFAEEDG
                     SIANSGRWLQWHWKGQDAPGEARNDGEILAGIYHHLRELYQAEGGKGVEPLMKMSWNY
                     KQPHEPQSDEVAKENNGYALEDLYDANGVLIAKKGQLLSSFAHLRDDGTTASSCWIYT
                     GSWTEQGNQMANRDNSDPSGLGNTLGWAWAWPLNRRVLYNRASADINGKPWDPKRMLI
                     QWNGSKWTGNDIPDFGNAAPGTPTGPFIMQPEGMGRLFAINKMAEGPFPEHYEPIETP
                     LGTNPLHPNVVSNPVVRLYEQDALRMGKKEQFPYVGTTYRLTEHFHTWTKHALLNAIA
                     QPEQFVEISETLAAAKGIANGDRVTVSSKRGFIRAVAVVTRRLKPLNVNGQQVETVGI
                     PIHWGFEGVARKGYIANTLTPNVGDANSQTPEYKAFLVNIEKA"
     gene            9211..10092
                     /gene="yddG"
                     /locus_tag="NBI74_003871"
     CDS             9211..10092
                     /gene="yddG"
                     /locus_tag="NBI74_003871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415990.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid efflux DMT transporter YddG"
                     /protein_id="HCD1934143.1"
                     /translation="MTRQKATLIGLIAIVLWSTMVGLIRGVSEGLGPVGGAAAIYSLS
                     GLLLIFTVGFPRIRQIPKGYLLAGSLLFVSYEICLALSLGYAATRHQAIEVGMVNYLW
                     PSLTILFAILFNGQKTNWLIVPGLLLALVGVCWVLGGDNGLHYDEIINNITTSPLSYF
                     LAFIGAFIWAVYCTVTNKYARGFNGITVFVLLTGASLWVYYFLTPQPEMVFSTPVMIK
                     LISAAFTLGFAYAAWNVGILHGNVTIMAVGSYFTPVLSSALAAVLLSAPLSFSFWQGA
                     LMVCGGSLLCWLATRRG"
BASE COUNT         2430 a         2652 c         2559 g         2473 t
ORIGIN      
        1 atatcatgga gttaaaatat aaaatcatca accaggacta atcttaacaa cgaagcagca
       61 aatatttgcc atgctgaatg tgctttataa atctgcgatc cgtagcagac accataaata
      121 cacagacacg gagaatcact atgtttactt attatcaggc agaaaattca acagcagaac
      181 ccgctctggt taatgccatt gagcaaggcc ttcgggcaga gcacggtgtt gtcactgaag
      241 atgacattct catggagctg accaaatggg tggaagcttc tgataacgac atcctcagtg
      301 atatctacca gcaaacgatc aattatgtgg tcagtggtca gcaccctacg ctttaaggtg
      361 ctatgcttga tcggcaacct aatttagggg tttagcacgt gtttcttcgc tacggcgatg
      421 ttgtccttaa aactagcaac aggattgagg agttaaaatg aaatcgaacc gtcaggcacg
      481 tcatattctt ggactggacc ataaaatttc taaccagcgc aaaatagtta ccgaaggtga
      541 caaatccagc gtggtaaata atcccaccgg cagaaaacgc cccgctgaaa agtaattcat
      601 aaccatcagt cctcaatgac gattaaacac cattgcctgc gcaatggtgt ttttgttttt
      661 atctgcttta tacttggtgc cgacgccctg gcggtaatgc aaagacgata aaagccccca
      721 gggatggata ttcaaaaaag agtgagtgac atggaaccaa aaacaaaaaa acagcgttcg
      781 ctttatatcc cttacgctgg ccctgtattg ctggaatttc cgttgttgaa taaaggcagc
      841 gccttcagca tggaagaacg ccgtaacttc aacctgctgg ggttactgcc ggaagtggtc
      901 gaaaccatcg aagaacaagc ggaacgagca tggatccagt atcagggatt caaaaccgaa
      961 atcgacaaac acatctacct gcgtaacatc caggacacta acgaaaccct cttctaccgt
     1021 ctggtaaaca atcatcttga tgagatgatg ccagtcattt acaccccaac cgtcggcgca
     1081 gcctgtgagc gtttttctga gatctaccgc cgttcacgcg gtgtgtttat ctcttaccag
     1141 aaccgccaca atatggacga tattctgcaa aacgtaccaa accataatat caaagtgatt
     1201 gtggtgactg acggcgaacg tattctgggg cttggtgacc agggcatcgg cgggatgggc
     1261 attccgatcg gtaaactgtc gctctatacc gcctgtggcg gcatcagtcc ggcctatacc
     1321 cttccggtgg tgctggatgt cggcaccaac aaccaacagc tgcttaacga tccgctgtat
     1381 atgggctggc gtaatccgcg tatcactgac gatgaatact atgaattcgt tgatgaattt
     1441 atccaggctg tgaaacaacg ctggccggac gtactgttgc agtttgaaga cttcgcacaa
     1501 aaaaatgcga tgccgttact taaccgctat cgcaatgaaa tttgttcttt taacgatgac
     1561 attcagggca ccgcggcggt aacagtcggc acactgatcg cagccagccg cgcggcaggc
     1621 ggtcagttaa gcgagaaaaa aatcgtcttc cttggcgcag gttcagcggg atgcggcatt
     1681 gccgaaatga tcatcgccca gacccagcgc gaaggattaa gcgaggaagc ggcgcggcag
     1741 aaagtcttta tggtcgatcg ctttggcctg ctgaccgaca agatgccgaa cctgctgcct
     1801 ttccagacca aactggtgca gaagcgcgaa aatctcagtg actgggatac cgacagcgat
     1861 gtgttgtcac tgctggatgt ggtgcgtaat gtaaaaccag atattctgat tggcgtctca
     1921 ggacagaccg ggctgtttac ggaagagatc atccgtgaga tgcataaaca ctgtccgcgt
     1981 ccgatcgtga tgccgctgtc taacccgact tcacgcgtgg aagccacacc gcaggacatt
     2041 atcgcctgga ctgaaggtaa cgcgctggtc gccactggca gtccgtttaa tccagtggta
     2101 tggaaagata aaatctaccc tatcgcccag tgtaacaacg cctttatttt cccgggcatc
     2161 gggctgggtg ttattgcttc cggcgcgtca cgtatcaccg atgagatgct gatgtcggca
     2221 agtgaaacac ttgctcagta ttcgccgctg gtcctgaacg gcgaaggtct ggtactaccg
     2281 gaactgaaag atattcagaa agtctcccgc gcaattgcct ttgcggttgg caaaatggcg
     2341 cagcagcaag gcgtggcggt gaaaacgtct gccgaagctc tgcaacaggc cattgatgat
     2401 aatttctggc acgccgaata ccgcgactac cgccgtacct ccatctaagc ctgcgcccgg
     2461 tagtgagggc taccgggcaa ttttcctctc cctttttcag atctcatcca tactgggtag
     2521 tggcgaataa atctcatttg cctcacctgc tatgcagaac atcatccgaa aaggaggaac
     2581 tatgaaggct gcagttgtta cgaaggatca tcatgttgac gttacggata aaacactgcg
     2641 ctcactgaaa catggcgaag ccctgctgaa aatggagtgt tgtggtgtat gtcataccga
     2701 tcttcatgtt aagaatggcg attttggtga caaaaccggc gtgattctgg gacacgaagg
     2761 tattggtgtg gtggcagaag ttggccctgg tgttacttca ttaaaaccag gcgatcgtgc
     2821 cagcgtggcg tggttctatg aaggatgcgg tcattgcgaa tactgtaaca gtggtaacga
     2881 aacgctctgc cgttcagtta aaaatgccgg atacagcgtt gatggcggga tggcggaaga
     2941 gtgcatcgtg gtcgccgatt acgcggtaaa agtgccagat ggtctggact cggcggcggc
     3001 cagcagcatt acctgtgcgg gggtcactac ctacaaagcc gttaagctgt caaaaattcg
     3061 tccaggacag tggattgcta tctacggtct tggcggtctg ggtaacctcg ccctgcaata
     3121 cgcgaagaat gtctttaacg ccaaagtgat cgccattgat gtcaatgatg aacagttaaa
     3181 actggcaacc aaaatgggtg cagatttagc gattaactca cgcaccgaag acgccgccaa
     3241 aattgtgcag gagaaagccg gtggcgctca cgcagcggtg gtgacagcgg tagctaaagc
     3301 tgcgtttaac tcggcggtgg atgctgtccg tgcaggtggt cgtgtcgtgg ctgtcggtct
     3361 gccgccggag tctatgagcc tggacatccc acggctggtg ctggatggta ttgaagtggt
     3421 cggttcgctg gtcggcacgc gtcaggatct aaccgaagcc ttccagtttg ccgccgaagg
     3481 caaagtggtg ccgaaagtcg ccctgcgtcc gttagaggac atcaacacca tctttaccga
     3541 gatggaagaa ggcaaaatcc gtggccgtat ggtgattgat ttccgtcgct aagaggtctt
     3601 tgctgcgact gccatgttcg ggtcgcagcc tgtttcgcga gacgctttcc gtaatttcac
     3661 tcttcttctt ttcctccccc atatttcccc tcacaattcg cgaaaacgta gcgaaatttg
     3721 ttgcgctgca cggaacaata gtgatagtgt tccttttgag ggaacaagga tgaagagatg
     3781 ataatgaatt tcagacacaa gggattgcgt gacttgtttc ttctcggcaa aacctccggc
     3841 gttataccga cgcaagtcaa acgattacgc caccactcgc tgtgattgat gcagcatgct
     3901 gtcttgctga tatcgatatg cccggttacc gactacatcc gttaagcggc gatcgcgatg
     3961 gaatttgggc gatatctgtc tcgggcaact ggcgaatcac atttgaattc gtcaatggcg
     4021 atgcatacat actggattac gaggactatc actgatgaaa atggccaatc atccccgccc
     4081 gggggacatt attcaggaat cactggacga acttaatgtc agcctgcgcg agtttgccag
     4141 agcaatggaa attgcgccct caacggcaag ccgattgctg accggaaaag cagctttgac
     4201 gccagaaatg gcaataaaac tttccgtggt gatcggcagt tcgccgcaaa tgtggctgaa
     4261 tctgcaaaat gcctggagtc tggcagaggc ggaaaaaacg gtggatgtgt cgcgactgcg
     4321 ccgtctggtt acgcaataaa aaatggcgct tcttaaagcg ccattttcag cttaaagttc
     4381 aaaagagatt atatcccttc ttcactctct tttttcgctt ctgccttctc gatttcacga
     4441 taccagcgcg gatggtgttt cttcgcccag cgacggctca ccttcccttc gatcatccct
     4501 ttaatcgatc ctttcaccca gaatgccata tacatatgga tcaggatggc gtggatcagg
     4561 atgatacccg cagccgcatg gatcagcagg ctataacgga caacctgtat cgggaagtac
     4621 tgcgcaaagt acggacgcca gataatcact ccggtcacca gcagcacgaa aatcatgctc
     4681 atgattgacc agaacatcat cttttgcccg gcgttgtact taccgacatc cgccactttg
     4741 tgctcattgc cttttaatac ttcgacaatg ttcaacagcc acggaatatc tttcttatcc
     4801 gggatgttgt gatgcacaaa acgcacaaac ataaacatca gcgcgacgaa aatcgcaatg
     4861 ccgaagaacg ggtgcaaaat gcgtcccatc tgcggcgtac cgaaggtttg cgtcagccat
     4921 tgcagcgtcg ggaagaagaa cgaaatcccg gacagcgcca ccaggaagaa gcaaatcacc
     4981 acggtccagt gacaggcgcg atcaataaat ttggtgcgca caatcatttt cgacttactc
     5041 atgatttccc tcctcctcat cgtccacttc cttattcggg ccaataccga tgtagtggaa
     5101 aatcaacccg gcaaaagtgg caataaagcc agccgctgcc agcggtttca acgcgccttt
     5161 ccacaggctt accgaggtat cgatcttcgg atctttcggc agaccatgat acagctccgg
     5221 ctgatcggcg tgatgcagca cgtacataac gtgcgtacca ccgacccctt ccgggttgta
     5281 gacgccagca tgttcgtaac cacgcgcttt cagtttcgcc acgcgctgtt ccgccagctc
     5341 cagcatctcc ttcttggtgc cgaagtggat agccccggtc ggacaggttt tcacacaagc
     5401 cggttcctgg ccgacgctga cgcgatcgac acagagcgtg catttatata cccggttatc
     5461 ctctttgttg aggcgcggaa tattaaacgg acacccggca atgcagtaac cacagccgat
     5521 gcagttttcc gactggaaat cgacaatccc gttagcgtac tgaatgattg caccagcaga
     5581 cgggcatgcc ttcaggcagc ccggatcttc acagtgcata cagccgtctt tacggatcag
     5641 ccactccagc ttgccgttct gttcggtttc gctaaagcgc atcaccgtcc aggacttggc
     5701 gctcagatcg gcggggttat cgtaaacccc gacgcagtgc cccacttcat cacggatgtc
     5761 gttccactcc gaacacgcca cttgacaggc tttacagccg atacaagtgg aaacgtcgat
     5821 aagtttcgcg acttctgctt tgtaatcacg cacctgagaa ggcggcgtga tggagttagt
     5881 tgcggacctt ttgataatgt cctgcgtttc catagccatc tgttcgcccc cttacgcctt
     5941 ctcgatgttt actaagaacg ctttatattc cggcgtttgc gagtttgcat caccgacatt
     6001 cggcgtcaga gtgttagcga tataaccttt acgcgcgaca ccttcaaagc cccagtggat
     6061 tggaataccc accgtttcaa cctgctgacc gtttacattc agcggtttca gacggcgcgt
     6121 taccacagcc accgcgcgga taaagccacg cttgctggag acagtgacac gatcgccatt
     6181 ggcaatgcct ttcgccgccg ccagcgtttc gctgatttcc acaaactgtt ccggctgagc
     6241 aattgcgttg agcaatgcgt gcttggtcca ggtgtggaag tgctccgtca gacgataggt
     6301 cgtacccaca tacgggaact gctctttttt acccatccgc agcgcgtctt gttcatacag
     6361 acgaacaacc gggttagaca ccacgttcgg atgcaacggg ttagtgccca gcggcgtttc
     6421 aattggctcg tagtgttccg ggaacggacc ttccgccatt ttgttgatgg caaacaggcg
     6481 tcccatccct tccggttgca tgataaacgg cccggttggc gtacccggcg cggcattgcc
     6541 gaagtccgga atatcgttac ccgtccactt gctaccgttc cactggatca gcatccgttt
     6601 cggatcccac ggtttaccgt tgatatccgc cgaagcacgg ttgtagagta cgcgacggtt
     6661 gagcggccac gcccaggccc atcccagcgt attccccaga ccggacgggt cggagttatc
     6721 gcggttagcc atctggttgc cctgctcggt ccagctaccg gtgtagatcc agcaagaaga
     6781 tgcggttgta ccgtcatcac gcagatgtgc aaagctactc agcaactgac ctttcttcgc
     6841 aatcagcacg ccattagcgt catagagatc ttccagcgca tagccgttat tctctttcgc
     6901 cacttcatca gactgcggtt cgtgcggctg cttgtagttc cagctcatct tcatcagcgg
     6961 ttctacgcct ttaccacctt cggcctggta cagctcgcgc agatgatggt agatacccgc
     7021 cagaatttcg ccgtcgttac gtgcttcgcc cggcgcgtcc tgacctttcc agtgccactg
     7081 caaccagcga ccggagttag cgatagaacc atcttcttca gcaaagcagg tcgaaggcag
     7141 acggaatact tcagtctgaa tagatgccgg atcgacatcg ttcgactcgc cgtggttctg
     7201 ccagaaggta gaggtttcag tcaccagcgg atcgataacc accatgtact tcagcttgct
     7261 caggcagctc accactttgt ttttgtccgg gaaggacgca accgggttaa agccctggca
     7321 gaaataaccg gtgactttac cttcatccat catgttgaaa tacttgatga cgtcgtaggt
     7381 ctggtcccac ttcggcagcc agtcgtagcc ccagttgttc tctttctgcg cggcatcgcc
     7441 gtagaaagat ttcatcaggc taacgaaaaa cttcggatag ttgctccagt agttcacctg
     7501 atcagccagc gtcgctttcg gcgtgttcgc ttccagatac gactgcaaat caacctgttt
     7561 ttctgacggc agcgtcagat aacctggcag gctggtagag agcaggccta agtcagtcag
     7621 gccctgaatg ttggagtgac cacgcaatgc gttcacgccg ccaccggcca tacccatgtt
     7681 accgagcagc aactggatca tcgccatcgt acggatgttc tgcgcaccca cggtgtgctg
     7741 cgtccagccc agcgcgtaca ggaaggtggt tgtgcgatcc ggtgcgctgg tggaggccag
     7801 cacttcacac actttcagga agtcggcttt tggcgtaccg caaatgtttt ctactacgtc
     7861 cggcgtgtag cgggaaacgt gcgctttcag cagattccat acacagcgcg gatgagtcag
     7921 cgtgtcatcg cgtttcgcat agccgttttc atcgaactga tagttccagg acgacttatc
     7981 gtattgacgt ttttcagcgt cgtagccgct gaacagaccg tcttcgaaag caaaatcatc
     8041 acgcaccagc aggctggcgt tggtgtaatg cttaacgtat tcggcgttga ttttgttgtt
     8101 ttcgatcagg tagcgcaaaa cgccagacag gaacgtaatg tccgtaccgg aacgaatagg
     8161 tgcgtaaata tccgccacag aagcggtacg cgtaaaacgg ggatcgacaa cgatcaaggt
     8221 tgcatcgttg ttgtttttcg cttccatcgc ccagcggaaa ccgacgggat gcgcttcagc
     8281 agcgttaccg cccatcacca ttacgacgtt agcgtttttg atatccaccc agtggttggt
     8341 catcgcaccg cgaccaaatg ttggagcaag acttgctacc gttggtccgt gtcagacgcg
     8401 cgcctggttg tctaccgcca gcatcccgag ggagcgggca aatttttgag tcagcatccc
     8461 ggtttcgttg ctggcaccgg aagcacacag cataccggta gaaagccaac ggtttaccgt
     8521 tacgccctgc tcgttctttt caataaagtt agcgtcacgg tcggctttca tcagcttcgc
     8581 aatacgggag aatgcttctt cccagctaat gcgctgccat ttgtcagaac ctggcgcacg
     8641 atattccggg tagcgcagac ggttttcgct gttgacataa tccagcaaac cggccccttt
     8701 cgggcacagc gcaccacgac ttaccggatg atccgggtca ccttcaatgt gataaatcgc
     8761 ttctctggcg tttttcgcgc catcacccag gctatacatc aatagcccgc aacctacgga
     8821 acagtatgtg caggtgttac ggatctcttt cgcgcgtaat aatttgtagt ttcgcgcctg
     8881 agccagtgct tgcttcgggg caaagcccaa tgctgctact gttgttcccg ccataccgcc
     8941 cgcgcagatt ttaaaaaatt gtctgcgact gacgtccatt gctttcctct tttttcaggg
     9001 gtattactgc gcgaggaaac taacagtaaa attctgaatc tttttgcgcg aaatcaaaaa
     9061 gaacacgtgt ttgccacaaa ataatccgcc tgcgcaaatt gacctacctc aatagcggta
     9121 gaaaaacgca ccactgcctg acaggccagt taaaaaaatg ctataaaatt cagcttaatt
     9181 tttaacggca agagagacaa aacagcgagc atgacacgtc aaaaagcaac gctcataggg
     9241 ctaatagcga tcgtcctgtg gagcacgatg gtaggattga ttcgcggtgt cagtgaggga
     9301 ctcggcccgg tcggcggcgc agctgctatc tattcattaa gcgggctgct gttaatcttc
     9361 acggttggat ttccgcgtat tcggcagatc ccgaaaggct atttactcgc cgggagtctg
     9421 ttattcgtca gctatgaaat ctgtctggcg ctttccttag ggtatgcggc gacccgtcat
     9481 caggcgattg aagtggggat ggtgaactat ctatggccca gcctgacaat tctctttgcc
     9541 attctgttta atggtcagaa aaccaactgg ctgattgtac caggattatt attagccctg
     9601 gtcggcgtct gctgggtgtt aggcggtgac aatgggttac attatgatga aattatcaat
     9661 aatatcacca ccagcccatt gagttatttc ctggcgttca ttggggcgtt tatctgggca
     9721 gtctactgca cagtaacgaa taaatacgca cgcggattta acggaattac cgtttttgtc
     9781 ctgctaacgg gagcaagtct gtgggtttac tattttctta cgccacaacc agaaatggta
     9841 tttagcacgc ccgtcatgat taaactcatc tctgcggcat ttaccttagg atttgcttat
     9901 gctgcatgga atgtcggtat attacatggc aacgtcacca taatggcggt aggttcgtat
     9961 tttacgcccg tcctttcctc agcgcttgca gccgtgctgc tcagcgcccc gctgtcgttc
    10021 tcgttctggc aaggcgcgct aatggtctgc ggcggttccc tgctctgctg gctggcgaca
    10081 cgtcgtggct aaaataattg tcgggtctac ggcc
//
LOCUS       DAHHTG010000146        10101 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.147, whole genome shotgun sequence.
ACCESSION   DAHHTG010000146 DAHHTG010000000
VERSION     DAHHTG010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10101)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10101)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10101
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.147"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            complement(425..2998)
                     /gene="clpB"
                     /locus_tag="NBI74_003872"
     CDS             complement(425..2998)
                     /gene="clpB"
                     /locus_tag="NBI74_003872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002461515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HCD1934144.1"
                     /translation="MRLDRLTNKFQLALADAQSLALGHDNQFIEPLHLMSALLNQEGG
                     SVSPLLTSAGINAGQLRTDINQALNRLPQVEGTGGDVQPSQDLVRVLNLCDKLAQKRG
                     DNFISSELFVLAALESRGTLADILKAAGATTANITQAIEQMRGGESVNDQGAEDQRQA
                     LKKYTIDLTERAEQGKLDPVIGRDEEIRRTIQVLQRRTKNNPVLIGEPGVGKTAIVEG
                     LAQRIINGEVPEGLKGRRVLALDMGALVAGAKYRGEFEERLKGVLNDLAKQEGNVILF
                     IDELHTMVGAGKADGAMDAGNMLKPALARGELHCVGATTLDEYRQYIEKDAALERRFQ
                     KVFVAEPSVEDTIAILRGLKERYELHHHVQITDPAIVAAATLSHRYIADRQLPDKAID
                     LIDEAASSIRMQIDSKPEELDRLDRRIIQLKLEQQALMKESDEASKKRLDMLNEELSD
                     KERQYSELEEEWKAEKASLSGTQTIKAELEQAKIAIEQARRVGDLARMSELQYGKIPE
                     LEKQLEAATQLEGKTMRLLRNKVTDAEIAEVLARWTGIPVSRMMESEREKLLRMEQEL
                     HHRVIGQNEAVDAVSNAIRRSRAGLADPNRPIGSFLFLGPTGVGKTELCKALANFMFD
                     SDEAMVRIDMSEFMEKHSVSRLVGAPPGYVGYEEGGYLTEAVRRRPYSVILLDEVEKA
                     HPDVFNILLQVLDDGRLTDGQGRTVDFRNTVVIMTSNLGSDLIQERFGELDYAHMKEL
                     VLGVVSHNFRPEFINRIDEVVVFHPLGEQHIASIAQIQLKRLYKRLEERGYEIHISDE
                     ALKLLSENGYDPVYGARPLKRAIQQQIENPLAQQILSGELVPGKVIRLEVNEDRIVAV
                     Q"
     gene            complement(3128..3859)
                     /gene="pgeF"
                     /locus_tag="NBI74_003873"
     CDS             complement(3128..3859)
                     /gene="pgeF"
                     /locus_tag="NBI74_003873"
                     /EC_number="1.10.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphenol oxidase"
                     /protein_id="HCD1934145.1"
                     /translation="MSKLIVPQWPLPKGVAACSSTRIGGVSLPPYDSLNLGAHCGDNP
                     DHVGENRKRLFAAGNLPSKPVWLEQVHGKDVLKLIGEPYASKRADASYSNTPGTVCAV
                     MTADCLPVLFCNRAGTEVAAAHAGWRGLCAGVLEETVSCFADNPENILAWLGPAIGPR
                     AFEVGAEVREAFMAVDAKASTAFIQHGDKYLADIYQLARQRLASVGVEQIFGGDRCTY
                     TENETFFSYRRDKTTGRMASFIWLI"
     gene            complement(3856..4836)
                     /gene="rluD"
                     /locus_tag="NBI74_003874"
     CDS             complement(3856..4836)
                     /gene="rluD"
                     /locus_tag="NBI74_003874"
                     /EC_number="5.4.99.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017383977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(1911/1915/1917) synthase
                     RluD"
                     /protein_id="HCD1934146.1"
                     /translation="MAQRVQLTATVSENQLGQRLDQALAEMFPDYSRSRIKEWILDQR
                     VLVNGKVCDKPKEKVLGGEQVAINAEIEEEARFEPQDIPLDIVYEDEDIIVINKPRDL
                     VVHPGAGNPDGTVLNALLHYYPPIADVPRAGIVHRLDKDTTGLMVVAKTVPAQTRLVE
                     SLQRREITREYEAVAIGHMTAGGTVDEPISRHPTKRTHMAVHPMGKPAVTHYRIMEHF
                     RVHTRLRLRLETGRTHQIRVHMAHITHPLVGDPVYGGRPRPPKGASEAFISTLRKFDR
                     QALHATMLRLYHPISGIEMEWHAPIPQDMVELIEEMRADFEEHKDEVDWL"
     gene            4971..5708
                     /gene="bamD"
                     /locus_tag="NBI74_003875"
     CDS             4971..5708
                     /gene="bamD"
                     /locus_tag="NBI74_003875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HCD1934147.1"
                     /translation="MTRMKYLVAAATLSLFLAGCSGSKEEVPDNPPNEIYATAQQKLQ
                     DGNWRQAITQLEALDNRYPFGPYSQQVQLDLIYAYYKNADLPLAQAAIDRFIRLNPTH
                     PNIDYVMYMRGLTNMALDDSALQGFFGVDRSDRDPQHARAAFSDFSKLVRGYPNSQYT
                     TDATKRLVFLKDRLAKYEYSVAEYYTERGAWVAVVNRVEGMLRDYPDTQATRDALPLM
                     ENAYRQMQMNAQAEKVAKIIAANSSNT"
     gene            5978..6319
                     /gene="raiA"
                     /locus_tag="NBI74_003876"
     CDS             5978..6319
                     /gene="raiA"
                     /locus_tag="NBI74_003876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HCD1934148.1"
                     /translation="MTMNITSKQMEITPAIRQHVADRLAKLEKWQTHLINPHIILSKE
                     PQGFVADATINTPNGVLVASGKHEDMYTAINELINKLERQLNKLQHKGEARRAATSVK
                     DANFVEEVEEE"
     misc_feature    6422..6547
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            6423..6470
                     /gene="pheL"
                     /locus_tag="NBI74_003877"
     CDS             6423..6470
                     /gene="pheL"
                     /locus_tag="NBI74_003877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pheA operon leader peptide PheL"
                     /protein_id="HCD1934149.1"
                     /translation="MKHTPFFFAFFFTFP"
     gene            6569..7729
                     /gene="pheA"
                     /locus_tag="NBI74_003878"
     CDS             6569..7729
                     /gene="pheA"
                     /locus_tag="NBI74_003878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydratase"
                     /protein_id="HCD1934150.1"
                     /translation="MTSENPLLALREKISALDEKLLALLAERRELAVEVGKAKLLSHR
                     PVRDIDRERDLLERLITLGKAHHLDAHYITRLFQLIIEDSVLTQQALLQQHLNKINPH
                     SARIAFLGPKGSYSHLAARQYAARHFEQFIESGCAKFADIFNQVETGQADYAVVPIEN
                     TSSGAINDVYDLLQHTSLSIVGEMTLTIDHCLLVSGTTDLSTINTVYSHPQPFQQCSK
                     FLNRYPHWKIEYTESTSAAMEKVAQAKSPHVAALGSEAGGTLYGLQVLERIEANQRQN
                     FTRFVVLARKAINVSDQVPAKTTLLMATGQQAGALVEALLVLRNHSLIMTRLESRPIH
                     GNPWEEMFYLDIQANLESAEMQKALKELGEITRSMKVLGCYPSENVVPVDPT"
     gene            complement(7772..8893)
                     /gene="tyrA"
                     /locus_tag="NBI74_003879"
     CDS             complement(7772..8893)
                     /gene="tyrA"
                     /locus_tag="NBI74_003879"
                     /EC_number="1.3.1.12"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydrogenase"
                     /protein_id="HCD1934151.1"
                     /translation="MVAELTALRDQIDEVDKALLNLLAKRLELVAEVGEVKSRFGLPI
                     YVPEREASMLASRRAEAEALGVPPDLIEDVLRRVMRESYSSENDKGFKTLCPSLRPVV
                     IVGGGGQMGRLFEKMLTLSGYQVRILEQHDWDRAADIVADAGMVIVSVPIHVTEQVIG
                     KLPPLPKDCILVDLASVKNGPLQAMLAAHDGPVLGLHPMFGPDSGSLAKQVVVWCDGR
                     KPEAYQWFLEQIQVWGARLHRISAVEHDQNMAFIQALRHFATFAYGLHLAEENVQLEQ
                     LLALSSPIYRLELAMVGRLFAQDPQLYADIIMSSERNLALIKRYYKRFGEAIELLEQG
                     DKQAFIDSFRKVEHWFGDYAQRFQSESRVLLRQANDNRQ"
     gene            complement(8904..9974)
                     /gene="aroF"
                     /locus_tag="NBI74_003880"
     CDS             complement(8904..9974)
                     /gene="aroF"
                     /locus_tag="NBI74_003880"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroF"
                     /protein_id="HCD1934152.1"
                     /translation="MQKDALNNVHITDEQVLMTPEQLKAAFPLSLQQEAQIADSRKTI
                     SDIIAGRDPRLLVVCGPCSIHDPETALEYARRFKALAAEVSDSLYLVMRVYFEKPRTT
                     VGWKGLINDPHMDGSFDVEAGLQIARKLLLELVNMGLPLATEALDPNSPQYLGDLFSW
                     SAIGARTTESQTHREMASGLSMPVGFKNGTDGSLATAINAMRAAAQPHRFVGINQAGQ
                     VALLQTQGNPDGHVILRGGKAPNYSPADVAQCEKEMEQAGLRPSLMVDCSHGNSNKDY
                     RRQPAVAESVVAQIKDGNRSIIGLMIESNIHEGNQSSEQPRSEMKYGVSVTDACISWE
                     MTDALLREIHQDLNGQLTARVA"
BASE COUNT         2570 a         2674 c         2355 g         2502 t
ORIGIN      
        1 aagaattaaa cttcgtaatg aattacgtgt tcactcttga gacttggtat tcatttttca
       61 tcttgcgacg tcaagaatcc gtatcttcga gtgcccacac agattgtctg ataaattgtt
      121 aaagagcagt gccgcttcgc ttttctcagc ggcgcggggt gtgcataata cactttcccg
      181 ctacagagtc aagcatttat ttttgctttc tctgccggaa ttctcaggag aaccccgctg
      241 acccggcggc gtgtttgccg ttgttccgtg tcagtggtgg cgcattatag ggagttattc
      301 cggcctgaca agcaaaaaat ataaaaactt tattgttcgc tcatttttca ggcaaaacat
      361 ctttaatata gtcttttccg tctaacttat agacaaaaac gagccccgaa ggctcgtttt
      421 atcattactg gacggcgacg atccggtctt cattaacttc caggcgaata actttacccg
      481 gaaccaattc accagacagt atttgctgtg ccagcgggtt ttcgatctgc tgctggatag
      541 cacgtttcag aggacgtgca ccatagaccg gatcgtaacc gttctcgctc agcagtttca
      601 gcgcctcgtc agaaatgtgg atttcataac cacgttcttc cagacgtttg tacagacgtt
      661 tcaactgaat ctgcgcaatc gacgcaatgt gttgttcacc cagcggatgg aagacgacga
      721 cttcatcgat acggttaatg aattccggac ggaagttatg gcttaccaca ccgagcacca
      781 gctctttcat gtgcgcataa tccagttcac caaaacgttc ctgaatcaga tcggaaccga
      841 ggttagaggt cataatgacg accgtattac ggaagtcgac cgttctcccc tgcccgtcag
      901 tcagacgccc atcatccagt acctgcaaca gaatgttgaa gacatccgga tgcgcttttt
      961 ccacttcatc cagcaggatg acagaatacg gacgacgacg caccgcttcg gtcagatagc
     1021 caccttcttc ataaccaaca tatcccggag gcgcaccaac cagacgagac accgagtgtt
     1081 tctccataaa ctcggacata tcgatacgga ccatcgcctc gtcgctatca aacataaagt
     1141 tcgccagcgc cttacaaagc tctgttttcc ccacacccgt tgggccgaga aacaggaatg
     1201 aaccaatcgg gcgatttgga tccgccagcc ccgcacggct acgacgaata gcgttagata
     1261 ccgcatcaac cgcttcgttc tgaccaataa cgcgatggtg cagttcttgc tccatacgca
     1321 gcagtttttc gcgctcgctt tccatcatgc gagaaacagg gatccccgtc caacgcgcca
     1381 gcacttcagc aatttcggca tcggtcactt tattacgcaa cagacgcata gttttgcctt
     1441 cgagctgcgt tgcggcttcc agttgctttt ccagttccgg gattttgccg tattgcagtt
     1501 cagacatccg cgccaggtca cccacacggc gagcctgttc aatagcaatt ttcgcctgtt
     1561 ccagttccgc tttaatggtc tgtgtaccag aaagcgatgc tttctctgct ttccactctt
     1621 cttctaactc ggagtactga cgttctttgt cgctcagttc ttcgttgagc atatccaggc
     1681 gttttttact ggcttcatca gactctttca ttaacgcctg ttgttccagt ttaagctgga
     1741 tgatacgacg atcgagtcgg tcgagttctt ctggttttga gtcaatctgc atacgaatgc
     1801 tggatgctgc ttcatcgatc aggtcgatgg ctttatccgg cagctgacgg tcagcaatgt
     1861 agcgatgaga caacgtcgca gctgcaacaa ttgccgggtc agtaatttgc acatggtggt
     1921 gcagttcgta acgttctttt aagccacgca gaatagcaat ggtgtcttca acagaaggct
     1981 cggcaacaaa cactttctgg aaacgacgct ccagcgcagc atctttttcg atgtactggc
     2041 gatattcgtc aagcgtcgtg gcacctacgc agtgcaattc accacgcgcc agcgccggtt
     2101 tcagcatgtt tccggcgtcc attgcgccat cggctttacc cgcgccgacc atggtatgta
     2161 attcgtcgat aaacaggatg acgttgcctt cctgtttagc aagatcgtta agtacgcctt
     2221 ttaaacgttc ttcaaactca ccgcgatatt tcgccccagc caccagcgcg cccatatcca
     2281 gcgccagtac gcggcggcct ttcaaccctt cgggcacttc gccgttgata atacgctgcg
     2341 ccagaccttc aacgatggca gttttaccga cgccgggttc accaatcaat accgggttat
     2401 ttttagtacg acgttgcagc acctgaatgg tacggcgaat ttcttcatca cgaccaatca
     2461 ccggatcgag tttgccttgt tcggctcgtt cggtaaggtc gatggtatat tttttcaaag
     2521 cctgacgttg gtcttcagca ccttgatcgt tcacgctttc acctccacgc atttgttcaa
     2581 tcgcttgagt aatgttggcg gtggtcgccc ctgctgcttt caggatgtcg gccagcgtgc
     2641 cgcgagactc aagtgccgcc agaacgaaca gttctgacga gataaagtta tcaccacgtt
     2701 tttgcgccag cttgtcgcaa agattaagaa cgcgcaccag atcctgtgat ggctggacat
     2761 caccaccagt accttcaacc tgtggtaaac gatttaatgc ctgattgata tctgtgcgca
     2821 actggccagc attaatgcca gcggatgtta ataaaggact aaccgaaccc ccttcctgat
     2881 tcagcagggc gctcattaaa tgaagtggtt cgatgaattg gttgtcgtgc ccgagtgcaa
     2941 gtgattgggc atcggcaaga gcaagctgga atttattagt aagacgatcc agacgcataa
     3001 ctcctcccat aacggatcaa agttgctact ggagattaaa tgaggtcatc cctcaattat
     3061 tcaaggttat tgaccagatt aatgtgaaaa gaaaatcacg cgtaccggat cgtcttgatt
     3121 ctttaggtta tatcagccaa atgaaacttg ccatacgacc ggtggtcttg tcgcgacgat
     3181 aagagaagaa agtctcattt tccgtatatg tacaacggtc gccgccgaaa atttgttcaa
     3241 caccaacgct cgccagacgc tgccgggcaa gctgataaat atccgccaga tacttatcac
     3301 catgctgaat gaaagctgta ctcgctttgg cgtctactgc cataaacgcc tcgcgaacct
     3361 ccgcccccac ttcgaacgca cgtggcccaa ttgccggccc taaccaggcg agaatatttt
     3421 ccggattatc agcaaaacag gaaaccgtct cttccaacac gcctgcgcac agtccacgcc
     3481 agccagcatg agcggcggcg acctccgttc ccgcacgatt gcaaaacagc acgggcaggc
     3541 agtcggcagt catcactgcg caaaccgtgc caggcgtatt gctataagag gcatctgctc
     3601 gttttgaggc ataaggttcg ccaataagct taagcacgtc tttgccgtgt acctgctcaa
     3661 gccagaccgg tttagaaggc aaattgcccg cagcaaaaag tcgcttgcga ttctccccaa
     3721 cgtgatccgg gttatcgcca caatgggcac cgaggttgag cgagtcatac gggggtaagc
     3781 tcacaccgcc gatacgggta gagctacaag ccgcaacacc ttttggcagc ggccactgcg
     3841 ggacaatcag cttactcata accagtccac ttcatcctta tgctcttcga aatcggcgcg
     3901 catctcctca atcagctcca ccatatcttg tggaataggc gcatgccatt ccatttcgat
     3961 gccggagatc gggtgataaa gacgcagcat ggttgcatga agcgcctgac ggtcaaactt
     4021 acgcagcgtg gagataaacg cttccgaagc accttttggc ggacgcggac gtccaccata
     4081 aaccggatcg cccaccagcg gatgagtgat atgggccata tgcacgcgga tctggtgcgt
     4141 acgtccggtt tccagacgca accgcagacg cgtgtgcaca cggaagtgtt ccatgatgcg
     4201 atagtgagtg accgccggtt tacccatggg atgtaccgcc atgtgagtac gtttggtcgg
     4261 gtggcggctg attggttcat ctactgtacc gccagcggtc atatgaccaa ttgccaccgc
     4321 ttcatactca cgtgtaattt cacgccgttg caaagattcg actaaacgcg tctgagccgg
     4381 aacggttttt gccacaacca tcaggccagt ggtgtcttta tccagacgat ggacaatgcc
     4441 cgcacgcggt acatcggcaa tgggcggata ataatgaagc aatgcattca gtaccgtgcc
     4501 atccgggtta cccgcgccag gatgtaccac caggtcgcgc ggtttattaa taacgatgat
     4561 atcttcatct tcatagacga tatccagcgg gatatcctgc ggttcaaaac gcgcttcttc
     4621 ttcaatctca gcgttgatgg caacctgctc gccacccaat actttttctt tcggcttatc
     4681 acacactttg ccgttaacca gcacacgctg gtcgaggatc cattctttta tccgcgaacg
     4741 tgaataatcc gggaacattt cggccaaagc ctgatctaag cgttgaccga gttggttttc
     4801 ggacaccgtt gcagtgagct gtactcgttg tgccatatat actgcttctt cgtttatcgt
     4861 tggggtttta cggctttgcc gtttaatata gtgtgctatt gtagctggtc ttaaccggga
     4921 gcaggaacag agaatctccc gtattacatt ttgaggaaag tcaaaacgtc atgacgcgca
     4981 tgaaatatct ggtggcagcc gccacactaa gcctgttttt ggcgggttgc tcggggtcaa
     5041 aggaagaagt acctgataat ccgccaaatg aaatttacgc gactgcacaa caaaagctgc
     5101 aggacggtaa ctggagacag gcaataacgc aactggaagc gttagataat cgctatccgt
     5161 ttggtccgta ttcgcagcag gtgcagctgg atctcatcta cgcctactat aaaaacgccg
     5221 atttgccgtt agcacaggct gccatcgatc gttttattcg ccttaacccg acccacccga
     5281 atatcgatta tgtcatgtac atgcgtggcc tgaccaatat ggcgctggat gacagtgcgc
     5341 tgcaagggtt ctttggcgtt gaccgtagcg atcgcgatcc tcaacatgca cgagctgcgt
     5401 ttagtgactt ttccaaactg gtgcgcggct atccgaacag tcagtacacc accgatgcca
     5461 ccaaacgtct ggtattcctg aaagatcgtc tggcgaaata tgaatactcc gttgccgagt
     5521 attatacaga acgtggtgca tgggttgctg tcgttaaccg cgtagaaggc atgttgcgcg
     5581 actatccgga tacccaggct acgcgtgatg cgctgccgct gatggaaaat gcataccgtc
     5641 agatgcagat gaatgcgcaa gctgaaaaag tagcgaaaat catcgccgca aacagcagca
     5701 atacataaca gaaacctgaa acacaaaacg gcagccttga gctgccgttt ttttattctg
     5761 ttagttgtga aactgaagcg atttagtcac tatcgatctc atcaaatatg gctcgctttg
     5821 agatattcct caagtaaaaa aacacctctt cctgcgattt ctcacaaaaa agattcgttg
     5881 acaaaaagtg acaaaattat gagatttcca tcacacattt tgacatcagg aacgttatgc
     5941 tgaattcacc aagacgggaa gacaagaggt aaaatttatg acaatgaaca ttaccagcaa
     6001 acaaatggaa attactccgg ccatccgcca acatgtcgca gaccgtctcg ccaaactgga
     6061 aaaatggcaa acacatctga ttaatccaca tatcattctg tccaaagagc cacaagggtt
     6121 tgttgctgac gccacaatca atacacctaa cggcgttctg gttgccagtg gtaaacatga
     6181 agatatgtac accgcaatta acgaattgat caacaagctg gaacggcagc tcaataaact
     6241 gcagcacaaa ggcgaagcac gtcgtgccgc aacatcggtg aaagacgcca acttcgtcga
     6301 agaagttgaa gaagagtagt cctttatatt gagtgtatcg ccaacgcgcc ttcgggcgcg
     6361 ttttttgttg acagcgtgaa aacagtacgg gtactgtact gaagtcactt aaggaaacaa
     6421 acatgaaaca cacaccgttt ttcttcgcat tcttttttac cttcccctga atgggaggcg
     6481 tttcgtcgtg tgaaacagaa tgcgaagacg aacaataagg cctcccaaat cggggggcct
     6541 tttttattga taacaataag gcaacactat gacatcggaa aatccattac tggcgctgcg
     6601 agagaaaatc agcgcgctgg atgaaaaatt attagcatta ctggcagagc ggcgcgaact
     6661 ggccgtcgag gtgggaaaag ccaaattgct ctcgcatcgc ccggtacgtg atattgatcg
     6721 tgaacgcgat ttactggaaa gattaattac gctcggtaaa gcgcaccatc tggacgccca
     6781 ttacattact cgcctgttcc agctcattat tgaagattcc gtattaactc agcaggcgtt
     6841 gctccagcaa catctcaata aaattaatcc gcactcagca cgcatcgctt ttctcggccc
     6901 caaaggttct tattcccatc ttgcggcgcg ccagtatgct gcccgtcact ttgagcaatt
     6961 cattgaaagt ggctgcgcca aatttgccga tatttttaat caggtggaaa ccggccaggc
     7021 cgactatgcc gtcgtaccga ttgaaaatac cagctccggt gccataaatg acgtttacga
     7081 tctgctgcaa cataccagtt tgtcgattgt tggcgagatg acgttaacta tcgaccattg
     7141 tttgttggtc tccggcacta ctgatttatc caccatcaat acggtctaca gccatccgca
     7201 gccattccag caatgcagca aattccttaa tcgttatccg cactggaaga ttgaatatac
     7261 cgaaagtacg tctgcggcaa tggaaaaggt tgcacaggca aaatcaccgc atgttgctgc
     7321 gttgggaagc gaagctggcg gcactttgta cggtttgcag gtactggagc gtattgaagc
     7381 gaatcagcga caaaacttca cccgatttgt ggtgttggca cgtaaagcca ttaacgtttc
     7441 tgaccaggtt ccggcgaaaa cgacgttgtt aatggcgacc ggacaacaag ctggtgcact
     7501 ggttgaagcg ttgctggtac tgcgcaacca cagtctaatt atgacccgtc tggaatcacg
     7561 tccgattcac ggtaatccgt gggaagagat gttttatctg gatattcagg ccaatcttga
     7621 atcagcggaa atgcaaaaag cattgaaaga gttaggggaa atcacccgtt cgatgaaggt
     7681 attgggctgt tacccaagtg agaacgtagt gcctgttgat ccaacctgat gaaaaagtgc
     7741 cggatgatgt gaatcatccg gcactggatt attactggcg attgtcattc gcctgacgca
     7801 ataacacgcg gctttcactc tgaaaacgct gtgcgtaatc gccgaaccag tgctccacct
     7861 tgcggaaact gtcaataaac gcctgcttat cgccctgctc cagcaactca atcgcctcgc
     7921 cgaaacgctt atagtaacgt ttgattaacg ccagattacg ctctgacgac ataataatgt
     7981 cggcataaag ctgcggatcc tgagcaaaca gtcgcccgac catcgccagc tcaaggcggt
     8041 aaatcggcga agagagcgcc agtagttgct caagctgaac attttcttct gccaggtgca
     8101 acccataagc aaaagtagca aagtggcgca aagcctgaat aaacgccata ttctgatcgt
     8161 gctcgacagc gctaatacga tgcaaccgag cgccccagac ctgaatttgc tccagaaacc
     8221 attggtatgc ttccggctta cgtccatcac accagaccac aacttgcttt gccaggctac
     8281 cgctgtccgg gccgaacatc gggtgtaacc ccagcaccgg gccatcgtgc gccgccagca
     8341 tggcctgtaa tggtccattt ttcaccgatg ccagatcaac cagaatacaa tctttcggta
     8401 aaggcggtaa tttgccgata acttgctcag taacgtggat gggcacacta acaatcacca
     8461 ttccggcatc ggcaacaata tcagccgctc gatcccagtc atgttgctcc agaatccgca
     8521 cctgataacc cgagagtgtt agcatcttct cgaacagacg tcccatctga ccgccaccac
     8581 cgacgataac caccgggcgc agtgacggac aaagcgtttt aaatcctttg tcgttttcac
     8641 tggagtaaga ttcacgcatc acccgacgca aaacatcctc aatcagatct ggcggtacac
     8701 ccagagcttc cgcctctgca cgacgcgagg ccaacataga tgcctcgcgc tccggaacat
     8761 aaataggcag tccaaagcgg cttttcacct cgcccacttc agcaaccagt tccagacgct
     8821 tcgctaataa attcagcagc gctttatcga cttcatcaat ttgatcgcgt aatgcggtca
     8881 attcagcaac cataataaac ctcttaagcc acgcgagccg tcagctgccc gttcagatcc
     8941 tgatgaattt cacgcagcaa ggcatcggtc atttcccagc taatgcaggc atcggttacg
     9001 gatacaccgt atttcatttc actgcgcggt tgctcggaag actgattgcc ctcgtggata
     9061 ttactttcga tcatcagacc aataattgag cgattgccat ctttgatttg agcaaccacg
     9121 gattctgcca ccgcaggctg acggcgataa tctttattgg aattaccgtg gctgcaatct
     9181 accatcagag acgggcgcag tcccgcctgt tccatctctt tttcacattg cgcaacatcc
     9241 gcagggctat agtttggcgc tttaccaccg cgcaggatca catgcccgtc cggattcccc
     9301 tgagtttgta gcaacgcaac ctgccctgcc tggttaatgc caacaaaacg gtgcggctgg
     9361 gcggcggcgc gcatagcgtt aattgccgtt gccagactgc catcggtgcc gtttttaaaa
     9421 ccaaccggca tggaaagccc ggaggccatt tcacggtgag tttgcgattc cgttgtacga
     9481 gcaccaattg ctgaccagct aaacagatcg cccaggtatt gcgggctatt cggatctaac
     9541 gcttccgtcg ccagtggcag tcccatattc accagctcaa gcagcaattt acgcgcgatc
     9601 tgcagcccgg cttctacatc aaaagagcca tccatatggg gatcgttaat taaccctttc
     9661 cagccgacag tggtacgggg tttttcaaaa tagacgcgca ttaccagata gaggctatcg
     9721 ctgacctctg cggcaagggc tttaaatcga cgagcatatt ccagagcagt ttccggatca
     9781 tgaatggaac aaggaccaca tactaccagc agacgaggat cgcgcccggc gataatatct
     9841 gaaatggttt tacgcgagtc agcaatctgg gcttcttgtt gcaggcttaa tggaaaagcg
     9901 gccttcagtt gttccggagt cattaaaacc tgttcgtcgg taatatgtac gttattcagc
     9961 gcgtcttttt gcatgatggc gatcctgttt atgctcgttt gcgatagttg atcctcagcg
    10021 aggatgacgt aacgataaca cataaagtaa agttttcaat ccatatttcg tacacatttc
    10081 tttacgtaag caatttattc g
//
LOCUS       DAHHTG010000147         9985 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.150, whole genome shotgun sequence.
ACCESSION   DAHHTG010000147 DAHHTG010000000
VERSION     DAHHTG010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9985)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9985)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9985
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.150"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            complement(<1..467)
                     /gene="folK"
                     /locus_tag="NBI74_003881"
     CDS             complement(<1..467)
                     /gene="folK"
                     /locus_tag="NBI74_003881"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HCD1934153.1"
                     /translation="MTVAYIAIGSNLASPLEQVNAALKALGDIPESRILAVSSFYRTP
                     PLGPQDQPDYLNAAVALKTTLAPEELLNHTQRIELQQGRVRKAERWGPRTLDLDIMLF
                     GNEVINTERLTVPHYDMKNRGFMLWPLFEIAPELVFPDGTILENLLRKNKFHE"
     gene            complement(464..1882)
                     /gene="pcnB"
                     /locus_tag="NBI74_003882"
     CDS             complement(464..1882)
                     /gene="pcnB"
                     /locus_tag="NBI74_003882"
                     /EC_number="2.7.7.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000174644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HCD1934154.1"
                     /translation="MTLPRCTIFTRVANFCRKVLSREESEAEQAVARPQVTVIPREQH
                     AISRKDISENALKVMYRLNKAGYEAWLVGGGVRDLLLGKKPKDFDVTTNATPEQVRKL
                     FRNCRLVGRRFRLAHVMFGPEIIEVATFRGHHEGNVSDRTTSQRGQNGMLLRDNIFGS
                     IEEDAQRRDFTINSLYYSVADFTVRDYVGGMKDLKDGVIRLIGNPETRYREDPVRMLR
                     AVRFAAKLGMRISPETAEPIPRLATLLNDIPPARLFEESLKLLQAGYGYETYKLLCEY
                     HLFQPLFPTITRYFTENGDSPMERIIEQVLKNTDTRIHNDMRVNPAFLFAAMFWYPLL
                     ETAQKIAQESGLTYHDAFALAMNDVLDEACRSLAIPKRLTTLTRDIWQLQLRMSRRQG
                     KRAWKLLEHPKFRAAYDLLALRAEVERNAELQRLVKWWGEFQVSAPPDQKGMLNELDE
                     EPSPRRRTRRPRKRAPRREGTT"
     gene            complement(1921..2847)
                     /gene="gluQRS"
                     /locus_tag="NBI74_003883"
     CDS             complement(1921..2847)
                     /gene="gluQRS"
                     /locus_tag="NBI74_003883"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001432381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HCD1934155.1"
                     /translation="MLPPYFLFKEMTDTHYIGRFAPSPSGELHFGSLIAALGSYLQAR
                     ARQGRWLVRIEDIDPPREVPGAAETILRQLEHYGLHWDGDVLWQSQRHHAYREALAWL
                     HEQGLSYYCTCTRARIQSIGGIYDGHCRDLHHGPDNAAVRIRQQHPVTQFTDLLRGII
                     HADEKLAREDFIIHRRDGLFAYNLAVVVDDHFQGVSEIVRGADLIEPTVRQISLYQLF
                     GWKVPDYIHLPLALNPQGAKLSKQNHAPALPKVDPRPVLIAALHFLGQQVETHWQDFS
                     VEQILQSAVKNWTLTAVPESAIVNSTFSNASC"
     gene            complement(2884..3339)
                     /gene="dksA"
                     /locus_tag="NBI74_003884"
     CDS             complement(2884..3339)
                     /gene="dksA"
                     /locus_tag="NBI74_003884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002209354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HCD1934156.1"
                     /translation="MQEGQNRKTSSLSILAIAGVEPYQEKPGEEYMNEAQLAHFRRIL
                     EAWRNQLRDEVDRTVTHMQDEAANFPDPVDRAAQEEEFSLELRNRDRERKLIKKIEKT
                     LKKVEDEDFGYCESCGVEIGIRRLEARPTADLCIDCKTLAEIREKQMAG"
     gene            complement(3517..4221)
                     /gene="sfsA"
                     /locus_tag="NBI74_003885"
     CDS             complement(3517..4221)
                     /gene="sfsA"
                     /locus_tag="NBI74_003885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HCD1934157.1"
                     /translation="MEFSPPLQRATLIQRYKRFLADVITPDGRELTLHCPNTGAMTGC
                     ATPGDTVWYSTSDNTKRKYPHTWELTQSQSGAIICVNTLWANRLTKEAILNESISELS
                     GYSSLKSEVKYGAERSRIDFMLQADSRPDCYIEVKSVTLAENEQGYFPDAVTERGQKH
                     LRELMSVAAEGQRAVIFFAVLHSAITRFSPARHIDEKYAQLLSEAQQRGVEILAYKAE
                     LSAEGMALKKSLPVTL"
     gene            complement(4236..4766)
                     /gene="thpR"
                     /locus_tag="NBI74_003886"
     CDS             complement(4236..4766)
                     /gene="thpR"
                     /locus_tag="NBI74_003886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HCD1934158.1"
                     /translation="MSEPQRLFFAIDLPAEIREQIIHWRATHFPPEAGRPVAADNLHL
                     TLAFLGEVSAEKEKALSLLAGRIRQPGFTLTLDDAGQWLRSRVVWLGMRQPPRGLIQL
                     ANMLRSQAARSGCFQSNRPFHPHITLLRDASEAVTIPPPGFNWSYTVTEFTLYASSFA
                     RGRTRYTPLKRWALTQ"
     gene            4840..7269
                     /gene="hrpB"
                     /locus_tag="NBI74_003887"
     CDS             4840..7269
                     /gene="hrpB"
                     /locus_tag="NBI74_003887"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase HrpB"
                     /protein_id="HCD1934159.1"
                     /translation="MSSLPVAAVLPELLAALDGASQVLLSAPTGAGKSTWLPLQLLAH
                     PGINGKIILLEPRRLAARNVAQRLAELLNEKPGDTVGYRMRAQNCVGPNTRLEVVTEG
                     VLTRMIQRDPELSGVGLVILDEFHERSLQADLALALLLDVQQGLRDDLKLLIMSATLD
                     NDRLQQMLPEAPVVISEGRSFPVERRYLPLPAHQRFDEAVAVATAEMLRQESGSLLLF
                     LPGVGEIQRVQEQLTSRIGSDVLLCPLYGALSLNDQRKAILPAPQGMRKVVLATNIAE
                     TSLTIEGIRLVVDCAQERVARFDPRTGLTRLVTQRVSQASMTQRAGRAGRLEPGICLH
                     LIAKEQAERAAAQSEPEILQSDLSGLLMELLQWGCSNPAQMSWLDQPPTVNLLAAKRL
                     LWMLGALDGERLSAQGQKMAALGNDPRLAAMLVSAKNDDEAATAAKIAAILEEPPRMG
                     NSDLGVAFSRNQPAWQQRSQQLLKRLNVRGGEADSSLIAPLLAGGYADRIARRRRQEG
                     RYQLANGMGAMLDADDALSRHEWLIAPLLLQGSSSPDARILLALPVDIDELVQRCPQL
                     VQQSDTVEWDDAQGTLKAWRRLQIGQLTVKVQPLAKPSEDELHQAMLNGIRDKGLSVL
                     NWTAEAEQLRLRLLCAAKWLPEYDWPAVDDESLLATLETWLLPHMTGVHSLRGLKSLD
                     IYQALRGLLDWVMQQRLDSELPAHYTVPTGSRIAIRYHEDNPPALAVRMQEMFGEATN
                     PTIAQGRVPLVLELLSPAQRPLQITRDLSAFWKGAYREVQKEMKGRYPKHVWPDDPAN
                     TAPTRRTKKYS"
     gene            7363..9888
                     /gene="mrcB"
                     /locus_tag="NBI74_003888"
     CDS             7363..9888
                     /gene="mrcB"
                     /locus_tag="NBI74_003888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glycosyl
                     transferase/transpeptidase"
                     /protein_id="HCD1934160.1"
                     /translation="MAGNDREPIGRKGKPTRPVKQKVSRRRYEDDDDYDDYDDYEDEE
                     PMPRKGKGKGKGRKPRGKRGWLWLLLKLAIVFAVLIAIYGVYLDQKIRSRIDGKVWQL
                     PAAVYGRMVNLEPDMTISKNEMVKLLEATQYRQVSKMTRPGEFTVQANSIEMIRRPFD
                     FPDSKEGQVRARLTFDGDHLATIVNMENNRQFGFFRLDPRLITMISSPNGEQRLFVPR
                     SGFPDLLVDTLLATEDRHFYEHDGISLYSIGRAVLANLTAGRTVQGASTLTQQLVKNL
                     FLSSERSYWRKANEAYMALIMDARYSKDRILELYMNEVYLGQSGDNEIRGFPLASLYY
                     FGRPVEELSLDQQALLVGMVKGASIYNPWRNPKLALERRNLVLRLLQQQQIIDQELYD
                     MLSARPLGVQPRGGVISPQPAFMQLVRQELQAKLGDKVKDLSGVKIFTTFDSVAQDAA
                     EKAAVEGIPALKKQRKLSDLETAIVVVDRFSGEVRAMVGGSEPQFAGYNRAMQARRSI
                     GSLAKPATYLTALSQPKIYRLNTWIADAPIALRQPNGQVWSPQNDDRRYSESGRVMLV
                     DALTRSMNVPTVNLGMALGLPAVTETWIKLGVPKDQLNPVPAMLLGALNLTPIEVAQA
                     FQTIASGGNRAPLSALRSVIAEDGKVLYQSFPQAERAVPAQAAYLTLWTMQQVVQRGT
                     GRQLGAKYPNLHLAGKTGTTNNNVDTWFAGIDGSTVTITWVGRDNNQPTKLYGASGAM
                     SIYQRYLANQTPTPLNLVPPEDIADMGVDYDGNFVCSGGMRVLPVWTSDPQSLCQQSE
                     MQQQPSGNPFDQSSQPQQQQPAQQEQKDSDGVAGWIKEMFGSN"
BASE COUNT         2307 a         2536 c         2840 g         2302 t
ORIGIN      
        1 aattcatgaa atttattttt tcttaataag ttttctaata tcgttccatc aggaaagacc
       61 agctccggcg caatttcaaa cagtggccac agcataaagc cacgattttt catatcgtag
      121 tgcggaacgg tcagacgttc ggtatttatt acttcattac caaacagcat gatgtcgaga
      181 tccagcgtgc gcggtcccca gcgttcagct ttacggacgc gaccttgctg caattcgata
      241 cgctgtgtgt gattgagtag ctcttcaggg gcaagagtgg ttttcagcgc gacggcggca
      301 tttaagtaat cgggttgatc ttgcggcccc agaggtgggg tgcggtaaaa cgaagaaacg
      361 gcaagaatgc ggctttctgg aatatcgcct aatgctttca gggcagcatt gacctgctcc
      421 agcggagagg ccagattgct gcctatggca atatacgcca ctgtcatgta gtaccctcac
      481 gacgcggtgc gcgtttgcgt ggacgacgag tacgacgacg cggtgacggt tcttcatcca
      541 gttcgttaag catccctttt tggtctggcg gcgcggaaac ctggaactca ccccaccatt
      601 tcaccagacg ttgcagttca gcgttacgct caacttcggc tcgtaaggcc aacaggtcgt
      661 atgccgcacg gaacttagga tgctccagca gtttccatgc gcgtttaccc tgacgacggg
      721 acatacgcaa ctgcaactgc cagatatcgc gagttagcgt agtcagacgt ttcgggatcg
      781 ccagtgaacg gcaggcttcg tccagcacgt cgttcatcgc cagtgcgaaa gcgtcgtgat
      841 aggtcaggcc gctctcctga gcaattttct gtgccgtctc cagcagtgga taccagaaca
      901 tggcggcaaa caggaacgcc gggttcacgc gcatatcgtt atggatacgc gtatcggtat
      961 tcttcagcac ctgttcaatg atccgctcca tcgggctgtc gccattttcc gtgaagtagc
     1021 gggtaatggt cgggaacagc ggctggaaca gatgatattc acacagcagc ttataggttt
     1081 cgtaaccgta gcccgcttgc aacagtttca gcgattcttc aaacagacgt gccgggggga
     1141 tatcgttcag taaggttgcc aggcgtggga tcggctcggc ggtttccggg ctgatgcgca
     1201 tgcccaattt ggcggcaaaa cgaaccgcgc ggagcatacg taccggatct tcacggtagc
     1261 gcgtttccgg gttaccaatc agacggataa cgccgtcttt cagatccttc atgccgccaa
     1321 cgtaatcacg gacggtaaaa tctgccacgc tgtaatacag gctgttgata gtgaaatcgc
     1381 ggcgctgggc gtcttcttcg atggagccga aaatgttgtc gcgcagcaac ataccgtttt
     1441 gcccgcgttg ggaggtcgtg cggtcgctga cgttaccttc gtgatgtcca cggaaggtcg
     1501 caacttcgat aatctccggg ccaaacatca catgagccag acggaaacgg cgacccacca
     1561 ggcggcagtt acggaacagt ttgcgcacct gctctggcgt ggcgttggtg gtcacgtcaa
     1621 aatctttcgg ctttttgcca agtaacaggt cgcgcacgcc gccgccaacc agccaggctt
     1681 cgtatcccgc tttattgagc ctgtacatta ccttcagggc attttcactg atatctttgc
     1741 gggaaatagc atgctgctca cgcgggatca ccgtcacctg tggacgggcg actgcctgtt
     1801 cagcctcgct ttcctcgcgg cttagcacct tgcggcaaaa attagcgact cgggtaaaaa
     1861 tagtacacct cggtagtgtc aaacatcatt caggacaaaa aaatagcggc taatcatagc
     1921 tcagcatgac gcatttgaga atgttgaatt tacaattgcc gactcaggca cggcggtcag
     1981 cgtccagttt ttgacggctg actgaaggat ttgctcgacg ctgaaatcct gccagtgtgt
     2041 ttctacctgc tgacccagaa aatgaagtgc cgcgattaac accgggcgcg gatcgacttt
     2101 cggcaacgca ggcgcatgat tctgcttgga aagtttagcg ccttgtggat taagcgccag
     2161 cggtaaatgg atgtaatctg gcactttcca gccaaaaagc tggtacagcg agatttgccg
     2221 cactgtcggt tcgatcaaat ctgccccgcg gacaatttcg ctcacgccct ggaagtgatc
     2281 atccaccacc accgccaggt tataggcaaa caaaccatca cggcgatgaa taataaaatc
     2341 ttcccgtgcc agtttttcgt cggcgtgaat aatgcctcgc agcaggtcag taaactgggt
     2401 gaccggatgc tgctggcgga tacgtactgc ggcgttgtct ggtccatgat gcaaatcccg
     2461 gcaatgaccg tcgtaaatac cgccaatgct ttgaatacgc gcacgcgtgc aggtgcagta
     2521 ataacttagt ccttgctcat gtaaccaggc gagtgcttcg cgataggcgt ggtgacgttg
     2581 cgattgccag agaacgtcgc cgtcccagtg taggccgtaa tgttccagct ggcgcaggat
     2641 agtctctgcg gcaccgggaa cttcacgagg cgggtcgata tcttcaatgc gtaccagcca
     2701 gcgaccttgc cgggcgcgag cctgcaaata gctgccaagc gcagcaatca gagaaccaaa
     2761 atgaagctcg ccggaaggag agggggcgaa gcgaccaata tagtgtgtgt ctgtcatctc
     2821 tttgaacaaa aaataaggcg ggagcatttc ccgcctgtgg taaacgtgat ggaacggctg
     2881 taattagcca gccatctgtt tttcgcgaat ttcagccagc gttttgcagt cgatgcatag
     2941 atcggctgtc gggcgcgctt ccagacggcg aataccaatt tcaacaccgc aggattcgca
     3001 gtagccgaaa tcttcgtctt ccactttttt cagcgtcttc tcgatctttt tgatcagctt
     3061 acgctcgcga tcgcggttac gcagttcgag gctgaactct tcttcctggg ctgcacggtc
     3121 taccgggtcc gggaagttgg ctgcttcatc ctgcatatgt gtaacggtgc gatcgacttc
     3181 atccctgagt tgattacgcc atgcttccag aatacgacgg aagtgcgcca gctgggcttc
     3241 attcatatac tcttcgcccg gcttctcctg atatggttcc accccagcga tggcgagaat
     3301 actcagggac gatgttttac ggttttgccc ttcttgcatg ttgcttctcc ttaacacgca
     3361 ctatcgatcc ccatgttcgg gggaaaaatg aggccgctat aaatagcaga tgcttttccg
     3421 gatagcaatt atctaaacgt aacacttgac aactgtgtga ggaaaagcgt atttgcgcac
     3481 gcgaccagaa tgtaaattaa ccagttactt actctactac aatgtaaccg gtagtgattt
     3541 tttaagagcc atgccttcgg cagaaagttc cgctttgtaa gccagaattt ctacccccct
     3601 ctgttgagct tctgacaata gttgcgcgta tttctcatcg atgtggcgcg cgggtgaaaa
     3661 ccgtgtaatg gctgaatgca gcacggcgaa aaagataacc gcacgctggc cttcagccgc
     3721 tacgctcatc aactcccgaa ggtgtttctg acctcgttca gtgaccgcat cgggaaaata
     3781 tccctgttcg ttctccgcta acgtaaccga tttcacttca atatagcagt ctggacgcga
     3841 atccgcctgc aacataaagt caatacggct gcgttcggcg ccgtatttta cttcgctttt
     3901 cagcgagcta tagcctgaca gctctgaaat tgattcatta aggatagcct ctttcgtcaa
     3961 cctgttagcc caaagcgtat tgacgcaaat aattgcgccg ctttggcttt gagttaattc
     4021 ccaggtgtgt gggtatttcc gtttggtgtt gtctgaagtc gaataccaga cggtatcgcc
     4081 aggcgttgca caaccggtca tcgcacccgt attcgggcag tgtagcgtta attcgcgacc
     4141 atcgggtgtg atcacatcgg ctaaaaaacg tttgtaacgc tgaattagcg tcgcgcgctg
     4201 tagaggggga gaaaattcca ttgcgacaat ccttgttatt gcgttagcgc ccagcgtttt
     4261 agcggcgtgt agcgtgtgcg tccacgggca aacgaggagg cgtaaagggt gaactccgtc
     4321 accgtatacg accagttaaa acctggcggc gggattgtca ccgcctcgct ggcgtcgcgc
     4381 aataaggtaa tatgtggatg aaacggacga ttgctttgaa aacaaccgct gcgagcagcc
     4441 tgtgaacgga gcatattcgc cagctggatt aagccgcgtg gcggctgtcg catccctaac
     4501 cacaccacac gcgaacgcag ccattgaccg gcgtcatcaa gcgtgagtgt gaaaccaggt
     4561 tgacgaatcc gtccggctaa aagagaaagc gccttctctt tctctgcgct cacttcgcct
     4621 aaaaatgcca gagtcagatg caaattatcg gcggcgaccg gacgtcccgc ctcaggtggg
     4681 aagtgtgtgg cgcgccagtg gataatctgt tcgcggattt ctgcaggtaa gtcgatagca
     4741 aagaacagcc gttgcggttc agacatgtga ggcactcggt tatgaattac cgcgatgcta
     4801 caatgtggcg cgaagaatgt taaccctctg gagcgttttg tgtcgtcgtt gcccgttgct
     4861 gccgtcttac ctgagttact tgctgccctc gatggtgcat cgcaggtgtt attaagtgcg
     4921 ccgaccgggg ccgggaaatc aacctggctg ccgctgcaac tgctggcgca tcccggcatt
     4981 aacgggaaaa ttatcctgct ggagccgcgt cgtctggcgg cgcgtaacgt tgcgcaacga
     5041 ctggcggagc tgcttaacga aaagccaggc gataccgttg gctaccggat gcgtgcgcaa
     5101 aactgcgtcg ggccgaatac ccgcctggaa gtggttaccg aaggcgtgct gacgcggatg
     5161 atccagcgtg acccggaact gagcggtgtt ggattggtga tccttgatga atttcatgag
     5221 cgcagcttgc aggcggattt ggcgttggcg ctgttactcg atgtgcaaca aggtctgcgt
     5281 gatgacctta aactgctgat tatgtcggct acgctggaca acgaccgctt gcagcaaatg
     5341 ctgccagaag cgcccgtcgt catctcagaa gggcgctcgt ttccagttga acgccgttat
     5401 ttgccgctgc ccgctcatca gcgttttgac gaagccgtcg cggttgccac cgccgaaatg
     5461 ctgcgtcagg aaagtggatc attactgtta tttttacctg gcgtcggaga aattcagcgt
     5521 gtgcaggaac aactgacttc acgcatcggc agtgatgtgt tgctctgccc gctgtatggc
     5581 gcgttgtcgc tgaacgatca gcgaaaagcg atcctcccgg caccgcaagg gatgcgtaaa
     5641 gtggtgctgg cgaccaatat tgctgaaaca agtttaacca tcgaaggtat tcgtctggtg
     5701 gtggattgtg cccaggagcg tgtggcgcgt tttgatccgc gtacagggct tacgcggctg
     5761 gttactcaac gtgttagtca ggcgtcgatg acgcagcgtg ccgggcgtgc cgggcgtctg
     5821 gagccgggta tctgcctgca tttaatcgcc aaagaacaag cagaacgcgc tgccgcgcaa
     5881 agtgaaccgg agatcttaca aagcgatctt tccggtttgc tgatggaatt actgcaatgg
     5941 ggatgcagca atccggcgca gatgagctgg ctggatcaac cgccaacggt gaatctactg
     6001 gccgcgaaac gcctgttatg gatgctgggg gcgctggacg gtgaacggct tagtgcgcaa
     6061 gggcaaaaaa tggcagcgct gggtaacgat ccgcgtttag cggcaatgct ggtgagcgcg
     6121 aagaacgacg acgaagctgc gaccgcggca aaaattgccg ccattctcga agagccgcca
     6181 cggatgggca atagtgacct gggcgtggcg ttttcgcgca atcaacccgc ctggcagcaa
     6241 cgtagtcagc aactgttaaa acgtttaaac gtacgcggcg gtgaggcaga cagttcgctt
     6301 atcgcgccgc tacttgccgg agggtatgcc gatcgtattg ctcgccgccg taggcaagag
     6361 ggacgctatc aactggcgaa cggcatgggg gcgatgctcg atgccgacga tgcgctaagc
     6421 cgccacgaat ggttgatcgc gccgttatta ttgcagggca gttcttcgcc ggatgcgcgg
     6481 attttactgg cgctgccggt cgatattgat gagttagtac aacgctgccc gcagctggta
     6541 cagcaatcgg acaccgtgga gtgggatgac gcgcaaggta cgctgaaagc ctggcgtcgg
     6601 ctgcaaatcg gtcagttgac ggtaaaagtg caaccgctgg cgaaaccctc ggaagacgag
     6661 ttgcatcagg cgatgcttaa cggcattcgt gataaaggtt taagcgtgct caactggacg
     6721 gcggaagcgg aacagctacg cttgcgtttg ttatgtgccg caaagtggtt gccggaatat
     6781 gactggccag cggttgatga tgaaagtttg ttggcgacgc tggaaacgtg gctactgccg
     6841 catatgacag gcgtacattc gctacgcggc ctgaaatcac tcgatattta tcaggcactg
     6901 cgtggattac ttgattgggt aatgcagcaa cgtctggata gtgaattgcc tgcgcattac
     6961 actgtgccga cgggaagccg gatcgccatt cgttaccatg aagataaccc gcccgcgctg
     7021 gcggtgagaa tgcaggagat gtttggtgag gccactaatc cgactatcgc ccaggggcgc
     7081 gtaccgttgg tgctggagtt gctttcccct gcccaaaggc cgctgcaaat cacgcgtgat
     7141 ttgagcgcct tctggaaagg agcgtaccgt gaggtgcaaa aagagatgaa agggcgttat
     7201 cccaaacatg tctggccgga cgacccggca aacaccgcac cgacgcgacg aacgaaaaag
     7261 tattcgtgaa gaccaaagca aatttgagag atatcttctt ctgtcttgca acagaagaac
     7321 agaaaatcgg gcttttgcgc ctgaatattg cggagaaaaa gcatggccgg gaatgaccgc
     7381 gagccaattg gacgcaaagg gaaaccgacg cgtccggtca aacaaaaggt aagccgtcgt
     7441 cgttacgaag atgacgatga ttacgacgat tatgatgact atgaggatga agaaccgatg
     7501 ccgcgcaaag gtaagggcaa aggcaaaggg cgtaagcctc gtggcaaacg cggctggtta
     7561 tggctgctgc taaaactggc tatcgttttt gccgtgctga tcgccattta cggcgtttat
     7621 ctcgatcaaa aaattcgcag ccgtattgat ggcaaggtct ggcaattgcc cgcggcagtt
     7681 tatggtcgaa tggtcaacct tgagccagac atgaccatca gcaagaacga gatggtgaag
     7741 ctgctggagg cgacccagta tcgccaggtg tcgaaaatga cccgtcctgg cgagtttacc
     7801 gtacaggcca acagcattga gatgatccgc cgtccgtttg atttcccgga cagcaaagaa
     7861 gggcaggtgc gcgcgcgtct gacctttgat ggcgatcatc tggcgacgat cgtcaatatg
     7921 gagaacaacc gtcagttcgg tttcttccgt cttgatccgc gcctgatcac catgatctct
     7981 tcgccaaacg gtgagcagcg tctgtttgtg ccgcgcagtg gtttcccgga tttgctggtg
     8041 gatactttgc tggcgacaga agaccgtcat ttctacgagc atgatgggat cagtctctac
     8101 tcaatcggac gtgcggtgct ggcaaacctg actgccggac gcacggtgca gggggcgagt
     8161 acgctgacgc aacagttggt taaaaacctg tttctctcca gcgagcgttc ttactggcgt
     8221 aaagcgaacg aagcctacat ggcgctgatc atggacgcgc gttacagcaa agaccgtatt
     8281 cttgagctgt atatgaacga ggtgtatctc ggtcagagcg gcgacaacga aatccgcggc
     8341 ttcccgctgg cgagcttgta ttactttggt cgcccggtag aagaattgag cctcgatcag
     8401 caggcgctgt tggtgggtat ggtgaaaggg gcgtccatct acaacccgtg gcgtaacccg
     8461 aaactggcgc tggagcgacg taacctggta ctacgtttgc tgcaacagca acaaattatt
     8521 gatcaagaac tctatgacat gttgagtgca cgtccgctgg gtgttcagcc gcgcggtggg
     8581 gtgatctctc ctcagccagc ctttatgcag ctggtgcgtc aggagctgca ggcgaaatta
     8641 ggcgataagg taaaagatct ctccggcgtg aagatcttca ctacctttga ctcggtggcc
     8701 caggacgcgg cagaaaaagc cgccgtggaa ggcattccgg cactgaagaa acagcgtaag
     8761 ttgagcgatc ttgaaaccgc gattgtggtt gttgaccgct ttagcggtga agttcgtgcg
     8821 atggtcggtg gctctgagcc gcagtttgcg ggctacaacc gtgcgatgca ggcgcgtcgt
     8881 tcgattggtt cccttgcaaa accagcgact tatctaacgg ccttaagcca gccgaaaatc
     8941 tatcgtttaa atacgtggat tgccgatgcg ccaatcgcgc tgcgtcagcc gaatggccag
     9001 gtctggtcac cgcagaacga tgatcgtcgt tatagcgaaa gtggcagggt gatgctggta
     9061 gatgcgttga cccgttcgat gaacgtgccg acggtgaatc tgggcatggc gttggggcta
     9121 cctgcggtta cggagacctg gattaaactg ggtgtgccga aagatcaact gaatccagtt
     9181 ccagcaatgt tgttgggggc gttgaactta acgccaatcg aagtggcgca ggccttccag
     9241 actatcgcca gcggtggtaa tcgtgctccg ctttccgcgc tacgttcagt catcgcagaa
     9301 gacggcaagg tgttgtatca gagcttcccg caggcggaac gcgctgttcc ggcgcaggcg
     9361 gcgtatctga cactatggac catgcagcag gttgtacaac gcggtacggg acgtcagctt
     9421 ggggcaaaat acccgaacct gcatctggca gggaaaacag ggactaccaa caataacgta
     9481 gatacctggt ttgcgggcat tgatgggagc acggtgacca tcacctgggt cggccgtgat
     9541 aacaaccagc cgaccaaact gtatggtgcc agcggggcaa tgtcgattta tcaacgttat
     9601 ctggctaacc agacgccaac accgctgaat cttgttccgc cggaagatat tgcagatatg
     9661 ggcgtggact acgacggcaa ctttgtttgc agcggtggta tgcgtgtctt gccggtctgg
     9721 accagcgatc cgcaatcgct gtgccagcag agtgagatgc agcagcaacc gtcaggcaat
     9781 ccgtttgatc agtcttctca gccgcagcaa cagcaacctg ctcaacagga gcaaaaagac
     9841 agcgacggcg tggctggctg gatcaaggag atgtttggta gtaattaata tcgtaatcgc
     9901 tctccgctta tgcggagagc gtaatgaaat catttagata aaccaaataa atatttattt
     9961 aatgaatata cgaccattaa tatat
//
LOCUS       DAHHTG010000148         9907 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.151, whole genome shotgun sequence.
ACCESSION   DAHHTG010000148 DAHHTG010000000
VERSION     DAHHTG010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9907)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9907)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9907
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.151"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            360..500
                     /locus_tag="NBI74_003889"
     CDS             360..500
                     /locus_tag="NBI74_003889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001418354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin expression modulating family protein"
                     /protein_id="HCD1934161.1"
                     /translation="MNSHTRYKLTLAELEAFNSAVDNRLAELTANFTSACWLQSCHLS
                     VR"
     gene            1140..1709
                     /locus_tag="NBI74_003890"
     CDS             1140..1709
                     /locus_tag="NBI74_003890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus Gp2 family protein"
                     /protein_id="HCD1934162.1"
                     /translation="MTYKYNPFWQQRIRETVRHALNVHPRLTALRVDLRFPDVPAATD
                     AAVISRFINALKARIDAYQKRKHREGKRVHPTTLHYVWAREFGECKGKKHYHLMLLVN
                     RDTWCRAGDYRAPGSLAGMIEQAWCSALGVDAGCHATLVHFPAWPAVWLARNDDTGFQ
                     QVLERADYLAKEHTKAHCTDERNFGCSRG"
     gene            1875..2276
                     /locus_tag="NBI74_003891"
     CDS             1875..2276
                     /locus_tag="NBI74_003891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004020115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1934163.1"
                     /translation="MYAKSFLALDSNGRLTGARTVQAAPYAHYTCHLCGSALRYHPQY
                     DTELPWFEHTDDRLTEHGQQCPYVRPERREIQLIKRLQQFVPDALPVVRKASWHCRQC
                     HHDYYGERYCTHCQTGGFSIPRTTQEEICEF"
     gene            2264..2674
                     /locus_tag="NBI74_003892"
     CDS             2264..2674
                     /locus_tag="NBI74_003892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HCD1934164.1"
                     /translation="MRILNCYMANDSKGHFVTAKEAAKHNRQDVLCCVSCGCPLTLQR
                     GNDGQPPWFEHDQMTVAEKILLRCTWLDPAEKEARRLHLQGMTVPDYTVKVRKWFCVM
                     CDEDYEGEKCCPRCGTGVYSREGGLQEGNWKDRN"
     gene            2958..4577
                     /locus_tag="NBI74_003893"
                     /pseudo
     CDS             2958..4577
                     /locus_tag="NBI74_003893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001368704.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            4657..4949
                     /locus_tag="NBI74_003894"
                     /pseudo
     CDS             4657..4949
                     /locus_tag="NBI74_003894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312320.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
     gene            complement(4989..6023)
                     /locus_tag="NBI74_003895"
     CDS             complement(4989..6023)
                     /locus_tag="NBI74_003895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotriesterase"
                     /protein_id="HCD1934165.1"
                     /translation="MKDYLQTVTGPVAREDMGLTLPHEHLFNDLSSVVDAPCYPFSQR
                     LVDKKVTAEIQWALKHDPYCCADNMDRKPIEDVIFEINNFISLGGRTIVDATGSESIG
                     RDAQALREVALKTGLNIVASSGPYLEKFESQRIHKTVDELAATIDKELNQGIGDTDIR
                     AGMIGEIGVSPTFTEAEHNSLRAASLAQINNPHVAMNIHMPGWLRRGDEVLDIVLGEM
                     GVSPNKVSLAHSDPSGKDVAYQRKMLDKGVWLEFDMIGLDITFPKEGIAPGVQETADA
                     VAHLIELGYADQLVLSHDVFLKQMWAKNGGNGWGFVPDVFLAYLAERGVDKTILKKLC
                     IDNPGRLLTA"
     gene            complement(6026..6991)
                     /locus_tag="NBI74_003896"
     CDS             complement(6026..6991)
                     /locus_tag="NBI74_003896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HCD1934166.1"
                     /translation="MKKNKYSTPLWMLATILAGMLSPMQSAVNGQLGHWLQDGNACAV
                     ISFASGLVVMFFIIMARKETRQQFAAIPSLIKNRKVPLWNWFAGLCGAMVVFSEGASA
                     SALGVATFQTALISALLLSGLLCDRVGIGVDEKKYFTPYRIIGALFAVIATIFVVSPQ
                     WHSTSFILLAILPFLAGLLAGWQPAGNAKVAEATGSMLVSITWNFIVGFCVLGTALAI
                     RVALGHVTVQLPDVWWMYLGGPLGLMSIGLMAILVRGLGLLMLGVASTAGQLLGSVLI
                     DVLIPSLGNTVYLVTIIGTLFALVGAIVTTIPEYRTSKTMKKMEV"
     gene            complement(7045..7803)
                     /locus_tag="NBI74_003897"
     CDS             complement(7045..7803)
                     /locus_tag="NBI74_003897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytoplasmic protein"
                     /protein_id="HCD1934167.1"
                     /translation="MNNTKKSLKVLFIGESWHIHMIHSKGYDSFTSSKYEEGATWLLQ
                     CLKNSQVDVTYMPAHTVQIAFPEDVAQLEQYDAIVISDIGSNTFLLQNDTFYKLRIKP
                     NALELIKEYVNNGGGLLMIGGYLSFMGIEAKANYKNTVLADVLPVTMLDGDDRVEKPE
                     GVIAQPSQPEHPVIKGFSEYPFFLGYNRAIAKENAEVVLTINNDPLLVFGNYHNGKIA
                     CFMSDCSPHWGTQQFMSWPFYTALWVNILTHIAR"
     gene            complement(7817..9031)
                     /locus_tag="NBI74_003898"
     CDS             complement(7817..9031)
                     /locus_tag="NBI74_003898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462448.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HCD1934168.1"
                     /translation="MKFERHHEILKRLSKFGSVKVSDLSNSLNVTKETIRSDLNELAR
                     LGYLTRCHGGAFIVLDSLDTIAKNEIAYALENYDTAQGIKKGHSTMKSQVCVIGSFNV
                     DIISYLPRLPTIGESLLASNFIFSPGGKGCNQALAASFADTDVYFITKVGTDHFSDYA
                     INFMNSSKIYKSIIYQTKETQTGTATILVNEGTGDNVIAIYPGANMTMSSDEITIQKE
                     AIINSDVILLQLETNYTALQQAITLAQKNSIPVIINPAPYNDIVNELIQDVDYITPNE
                     TEAGLLSGIDVHDLESAKRAAEAIHNKGVKNTVITLGSKGSLAFDGKKFIHSPAFPAV
                     VKNTAGAGDAFNGALASGLAKGKSLESALCYASAFASLAVETSNASDMPEHESVIHRI
                     QSIHYQQTIFTH"
     gene            complement(9227..9565)
                     /locus_tag="NBI74_003899"
                     /pseudo
     CDS             complement(9227..9565)
                     /locus_tag="NBI74_003899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080195.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         2632 a         2320 c         2417 g         2538 t
ORIGIN      
        1 taacgtaaag gatttgccag aataacctaa gagagtaaat ctctccggta acagagcgct
       61 gtgaagcatt ctgtatcaga gtgcaaatac atgaacgctg atgcgtaaaa tcagcagtga
      121 cacgccagct ccccctatgc ccattcctgt ataagacatg aactgagcgt attaatgctc
      181 ctgccctgag actgagccgg tgagtgaggg gctgagaaat ccctcttgac actgctctaa
      241 ccagaaatac ttccccggtt gttgcggtgc gtccggatcc ccgataacgg aacactaagg
      301 ctgtattgcg ggacaccagg taagttgcag cccgtaatac gtcaggagac gcgggaaaag
      361 tgaattccca cacgcgttat aaacttaccc ttgcggagct ggaagccttt aactctgccg
      421 ttgacaaccg gctggcagaa ctgaccgcaa attttacgag tgcgtgctgg cttcagtctt
      481 gtcacctgag cgttcggtaa cggaggcgcg ggcggtaata gactgatttt caggtattac
      541 cggcttacgg cgtgtggttg atattgaggg gctgtgagtt tcgcgtattt gtcagaaacc
      601 cagacgagtt ttcctgtatg ggacgaaata aggcagcggc aatacctgga aaaatttaat
      661 atggtatgaa ggagaacctg tcggttacgc ttaatatacc cggtacactg tcagctcagg
      721 ggatacatga accgttatct caccagctac acagtatata cacactgacg ctgacagtag
      781 tcagcaggac cacaataatc agtgccagca gtatggtaac gatacggtct gtattccggt
      841 aatagccgaa ggggtcgaca gaaccacaat acgggcaaca cactgtatta tgagtaatcg
      901 cgtttccaca gtccctgcac ttctttttgc gtatcaccat cactccctcc tcacagcctt
      961 acccgtaacc ggttttttac attaaaaaaa caacgctgaa aattcaaccg tcagtccgga
     1021 gacgaccgtt cgggttatca cagagtgcct gagacagtgc cctgccggag gtcacttcag
     1081 ggactctgcg cattttttac gacgtggcta ttccgtatga accatacgga gatttaacca
     1141 tgacctacaa atacaacccc ttctggcagc aacgtattcg tgagacagtg cggcacgcac
     1201 tgaatgttca tccccgcctg acggcattgc gggttgacct gcgtttcccg gatgtaccgg
     1261 cagcaacgga cgcagctgtg atatcccgct tcatcaatgc cctgaaagcc cgaatcgacg
     1321 cttaccagaa acgtaagcat cgggaaggta aacgcgtgca tcccaccact ctgcattacg
     1381 tctgggcccg ggagtttggg gagtgcaaag gtaaaaaaca ctatcatctg atgctgctgg
     1441 tcaaccggga tacctggtgt cgtgccggtg attaccgcgc tccgggatca ctggccggaa
     1501 tgattgaaca ggcctggtgc agtgccctgg gggtggatgc cggatgccat gccacgctgg
     1561 tgcattttcc ggcctggccg gcggtgtggc tggcgcgtaa tgatgacacc ggctttcagc
     1621 aagtgctgga acgtgctgac tatctggcga aggagcatac caaagctcac tgcaccgatg
     1681 agcgcaactt tggctgcagt cgtggctgag ccagacagac gagttatctg acttcagtgt
     1741 gatgacgtgt tcatggccac cacactgatt ttcccttatg agactgaact gaaaccgcca
     1801 ttcacacctg accacgctgc ccgtaccggg acggctgttg cctgcgtgtc gtctgacggt
     1861 aaggaacata tactatgtac gcaaaatcct ttctcgctct tgatagcaac ggacgtctga
     1921 cgggcgcccg tactgtacag gccgcacctt atgctcacta cacctgccac ttgtgtggca
     1981 gtgcactcag ataccatccg caatacgaca ctgaacttcc ctggtttgaa cacactgacg
     2041 acaggctgac agagcacggt caacagtgcc cttatgtcag gccggagcgt agagaaatac
     2101 agttgattaa acgtctgcag caattcgtac cggatgcctt acccgtggtg cgtaaagcca
     2161 gctggcactg cagacaatgt caccacgatt attatgggga gcggtactgc acacactgcc
     2221 agaccggagg attcagtatt ccccggacaa ctcaggagga aatatgcgaa ttctgaactg
     2281 ttatatggcg aatgacagca aaggccattt tgttacggcg aaagaagcag cgaagcacaa
     2341 ccgccaggac gttttgtgct gtgtgtcctg tggatgcccg ttaacacttc agcggggcaa
     2401 tgacggacaa ccaccgtggt ttgaacatga ccagatgact gtcgctgaga aaatcctgct
     2461 gcgatgcacc tggcttgacc cggcagagaa agaggcccgt cgtttgcatc tgcagggtat
     2521 gacggttccg gattatacgg tgaaggtgag aaagtggttt tgtgtgatgt gtgacgaaga
     2581 ttatgaagga gaaaagtgct gcccgcgttg tggtaccggg gtatacagca gggagggggg
     2641 gctgcaggaa ggcaactgga aggataggaa ctgagcggtt cagtcgcgga aatcagttgt
     2701 agagctgtaa tgatatctcc gggagtgctg cgttggtagg ggcatcccgg gatagagaaa
     2761 ttttagcact ttgccctgaa tgattgtaga cgtcccgggt actcgtaccg gtctgagtta
     2821 ttacagttag atattcactt tttctcccgc atacttcaca tctgtcaacg acggatgaaa
     2881 agtggttctt ccgcaacctg ggtggaagaa aatggatgaa actttctggc gaacaaggat
     2941 ctaagctgtt ttaagttatg ggcaacgcaa tgcactctct taagacactt ctacagttac
     3001 cttgcggatg gcgatgcagt cgacaaatta ttagctctga cggtataacc ctccatctcc
     3061 acggaaaacg caaaacagca caatgtcctg aatgctttaa gcgtagcgac tctgttcata
     3121 gttgtcgtcg gcgccggata cagcatctac cctgctcagg gcagacgcta tggcttgtat
     3181 tttccgtccg ccactggtac tgccgtaacc cggcttgtta acgtaaaatt tttgccgagt
     3241 cgcttgctcc cttcgccggt tcacaccagc agtcttcaca ggcgttacaa aatttacaac
     3301 gtcaactggg attaatagct ggaggtgagg ctggaaaacg ggctgcaacg gcagcgggtc
     3361 tccgttgcag tgctgatact cttcttcgca gggttatcaa taccccggag acgaaacagt
     3421 caggcgcgcc tcatgtcggt attgatgagt gggcgtggca tcggggccac tgctgcggta
     3481 tgttaatagt caatcttgat actcaccgtc ccctcgtcct gcttcccggc cgtgatcagc
     3541 gtacgctggc gacctggttc agaaaatatc cggaaataca ggttgtctcg cgtgatcgca
     3601 gtggagtcta tgcgacagca gcacgtgaag gtgcacctca ggccagacag gtggccgatc
     3661 gatggcacct gctaaaaagt attggtgatg agcctgaacg aatgatgtac agacatatgc
     3721 ctctgatacg tcttgttgtc agagagttat cactgaagaa atcacctgag ccagaaatat
     3781 ctgtgcctgt agcatcgctc cgtcgtccgg aacgccttaa acagcaaacc cgcaaaaaac
     3841 ggcatcagca ttggacagag gttatggccc tgcataacaa gggatgtagt ttcagggaaa
     3901 tatcccgtat tacaggcctg tcgcgagtga cagtcagtcg ctgggtgcgt tcaggaacat
     3961 tccctgaaat gtcaacccgg cctccaaagc gagggcttct ggacccatgg agggagtggt
     4021 taaaagagca acgagaaagt ggtaattata atgccagccg gatatggcgg gaaatggtgg
     4081 cccagggggg gacaggcagt gaaaccatcg tcagggatac tgttgccaaa tggcgtaaag
     4141 gctggaaccc accggttact actgccgcaa gacttccttc agtgtcccgg gtaagccgct
     4201 ggttgatgcc ctggagaata atcaggggcg aagaaaatta tgcatcccga tttattagtc
     4261 tgatgtgtga aaaagaaccg gagttgaaaa tagcacagca actggtactc gagttctacc
     4321 gtattctgaa aacctaaaat aaatcacagc taagcagctg gttcactcga gttcacgaaa
     4381 gcggctcagc agaacttcgg tgcgtagctg cggggatgga agctgatgct gcggctatat
     4441 gtgaggcaat cagcagtcgc tggagtaatg gtgttgtcga aggtcatgta aatcgcctga
     4501 aggtgctgat acgccagatg tatggtcgag ccggatttga actacttagg cggagggtaa
     4561 tgagtccact ggcatgaaaa tcttcaccta ggttgcggaa gagccacttt tgcactgttc
     4621 gtgacagtat ggcctttatg tactcacttt gttaccggta gctgaatcat cactaccgtg
     4681 ctgcaaatgg tggtgaacag catcaattgt ggaatgaatg ttgccgaagc gaccaatgcg
     4741 cctcgtttcc accatcagtg gttgccgggc tagctgcgtg ttgaaaaggg tttagtccgg
     4801 atacgctcaa gctgctggaa gcaaaaggtc agaaagtggc gctgaatgag gcgatgggca
     4861 gcacgcaaag cattatggtt gggtcggatg gtgagttgta cggcgcatcc gactcgcgct
     4921 tggtggatga tttaacggcg gggtattaat tgacatcccc cggcgcagtg tgccggggga
     4981 taatggtttt atgcggtcaa taatcgtccg ggattatcga tacagagttt tttgaggatc
     5041 gttttatcga cgccgcgctc cgccagatag gccagaaaaa catctggaac aaatccccag
     5101 ccattaccgc catttttagc ccacatttgt ttaaggaaga catcgtggct gagaacaagc
     5161 tgatcggcgt atcccaactc aatgagatga gcgacggcat cggcagtctc ctgcacccct
     5221 ggcgctattc cctctttcgg gaaggtaatg tctaggccaa tcatgtcgaa ttccagccag
     5281 acacccttat caagcatttt ccgctgatac gccacgtctt ttcctgacgg atccgagtgt
     5341 gcgagagaga ctttatttgg cgagacgccc atttcaccta acacaatgtc gagtacttca
     5401 tcaccgcgac gaagccagcc cggcatgtga atattcatcg ccacatgagg attgttaatc
     5461 tgtgccagcg aggcagcccg caagctgtta tgctcggctt cggtaaatgt cggtgagaca
     5521 cctatttcac cgatcattcc ggcacgaata tccgtatcgc caatcccttg attcaattct
     5581 ttatcgatcg ttgccgccag ttcatcaacc gttttatgaa ttctctggct ttcaaatttt
     5641 tccaggtagg gaccggagga agcaacaata ttcagacctg tttttaatgc gacttctcgt
     5701 aatgcctgcg catctcgtcc gatagactca gaccctgtgg catcaacaat ggtgcgacca
     5761 ccgagcgaga taaagttatt aatttcgaag atcacatctt caataggttt gcggtccata
     5821 ttatcagcgc agcaataagg atcgtgtttt aatgcccact ggatttctgc cgtgactttt
     5881 ttatcaacaa gccgttgtga aaaaggatag caaggagcat ccacaacaga ggaaagatcg
     5941 ttgaacagat gttcatgagg aagtgttagc cccatatctt cacgggcgac ggggcccgtc
     6001 acagtttgta gataatcttt catgattata cctccatttt cttcattgtt ttcgaggttc
     6061 tgtattccgg aatagtggta acaattgcac cgaccagtgc aaacagtgtt ccgatgatcg
     6121 tgaccagata aacggtattg cccagcgatg gaattaatac gtcaatcaat actgatccca
     6181 gtaactggcc cgccgttgaa gcaaccccca gcatcagtaa ccccagacct ctgaccaaaa
     6241 tggccattaa accgatagac attagtccaa gcgggccgcc aagatacatc caccagacat
     6301 ctggtaattg aacagtgacg tgccctaatg cgacacgaat agctagcgct gtgcctaaaa
     6361 cacaaaatcc aacgatgaaa ttccaggtga ttgaaaccag catggatccc gtagcttcag
     6421 ctacttttgc atttccagcg ggctgccatc ctgcaagcaa accagctaag aacggaagaa
     6481 tggcaagtaa gatgaaagag gtagaatgcc actgcggtga cacaacaaaa atcgtagcaa
     6541 taaccgcaaa taatgcaccg atgatgcgat aaggggtaaa gtattttttc tcgtcgacac
     6601 cgataccaac gcgatcgcaa agtaaaccag acagcaatag cgcggagatc agtgcggttt
     6661 gaaacgttgc gaccccgagt gcgctggctg atgcgccttc agaaaatacc accatcgctc
     6721 cacagagacc ggcaaaccag ttccagagag gaacttttcg gtttttaatc agagaaggaa
     6781 ttgccgcaaa ttgttgccgg gtttcttttc gcgccataat aataaaaaac atcacgacta
     6841 aaccactggc aaaggagata actgcgcatg cattaccatc ctgtaaccag tgacctaatt
     6901 gaccattcac cgctgattgc ataggggaga gcatacccgc aagaatcgtg gcaagcatcc
     6961 ataagggagt tgagtactta ttctttttca tacatcaatc cttctattat agtaaatggg
     7021 gtaactggag ttaccccatt attattatct tgcaatatga gtcagtatat ttacccacaa
     7081 cgcggtatag aacggccagg acataaactg ctgtgtaccc cagtgcggtg aacagtcact
     7141 cataaagcag gctattttcc cgttgtgata gttaccaaag accagtaatg gatcgttgtt
     7201 tattgttaat accacctcag cattttcttt agcgatcgcc cggttataac ctaggaaaaa
     7261 aggatattca gagaaccctt taataactgg atgttcaggt tgtgatggct gtgcaataac
     7321 accctctggt ttttcgacac gatcgtcacc atctaacatc gtaaccggga gcacatcagc
     7381 cagaactgta tttttataat tggcttttgc ttcaattccc atgaatgaga gatagccgcc
     7441 aatcatcaat aacccaccac cgttattcac atattcttta atcagttcga gagcgtttgg
     7501 ttttatgcga agtttataaa aagtatcatt ttgtagcagg aaagtattac taccaatatc
     7561 actaataacg atggcatcat attgttctaa ctgtgccaca tcttcaggga aggcaatttg
     7621 taccgtatgt gctggcatat aggtgacatc tacctgacta tttttcagac attggagtaa
     7681 ccaggtcgca ccttcttcat atttacttga ggtaaaactg tcatatcctt ttgaatgaat
     7741 catatgaata tgccaggatt ccccaataaa gagcactttt agtgattttt tggtgttatt
     7801 cattctctgt cccttcttaa tgcgtaaaaa tagtttgctg ataatgtata ctctgaattc
     7861 gatgtatcac agattcatgt tcaggcatgt cggaagcatt gcttgtctcc accgctaggg
     7921 atgcaaaggc gctggcataa cacagtgctg attccagtga ttttcctttc gcaagtcctg
     7981 atgccagagc gccgttaaaa gcatccccgg ctcctgccgt atttttaacg accgctggaa
     8041 acgctggcga atgaatgaat tttttgccat caaatgcgag cgagcccttg ctaccgagcg
     8101 tgataaccgt gttttttacc cctttattgt ggatagcttc tgcagctcgt ttagctgatt
     8161 caagatcgtg tacgtctatg ccggacagta aaccagcttc tgtttcattg ggggtgatat
     8221 aatcaacatc ctgaataagt tcgttgacga tatcattata gggtgccggg ttaataatga
     8281 ccgggatact gtttttttgc gccaatgtga tcgcttgttg cagagcggta taatttgttt
     8341 cgagctgcag aagtatgaca tcagaattaa taatcgcttc tttctgaata gttatttcat
     8401 cggacgacat tgtcatgttg gcaccgggat agatcgcaat aacattatcg ccagtacctt
     8461 cattaacaag gatcgtggca gttccggttt gcgtttcttt cgtttgataa attatacttt
     8521 tataaatctt agacgaattc ataaagttga tcgcataatc actaaagtga tctgtaccta
     8581 cttttgttat gaaataaaca tcagtatcgg caaagcttgc tgccagcgct tggttacagc
     8641 cttttccccc cggtgaaaat ataaaattac ttgccagaag cgattctccg atagtgggaa
     8701 gcctcggcaa ataactgatg atatcaacat taaacgaacc aataacgcat acttgacttt
     8761 tcatagttga gtggcctttt tttattccct gggctgtatc ataattttca agcgcataag
     8821 cgatttcatt tttcgcaata gtatcaagcg agtctaggac gataaaagca ccgccatgac
     8881 atcgagtcag atagccaagc ctggcaagtt cattaagatc tgatcgaata gtttctttcg
     8941 ttacattgag gctattggac agatcggata cttttacaga accgaattta ctgagtcgtt
     9001 tcaatatttc atgatggcgt tcaaatttca tgcttcacct cacttcagtt acgttaatat
     9061 ctatcgatgt atattgatgt gataatcacc ataaagtggc ggggaaaagc aaaaaaaagc
     9121 aaaaatagcc tgagaaaagg taagcgttaa tggagaatca tattgatgct tatttattgg
     9181 tgagaactac gttccatggc aggagttcat caatacggtt ggaaggttat tccggcagta
     9241 cgctcaggat atggcgcaga tacgcttccg gatcaatgtc gttcagaagg caagtgccga
     9301 taagcccata caacagtgca ctgcgctcgc cgccgaagaa cataaagttt ttatttccga
     9361 gacagatggc acaaagggct ttttccgcgg cattatgtct gcctccgcct gaccgtcaac
     9421 actgtaataa cagagtgcgc cccactgatt cagtacacag ctgaaccatt cacccagtct
     9481 ggatttttgg atagcgtggc atttttctcc accatgcatg cagtgagtcc agtaacagtt
     9541 tgctcttctt ctgcctgacc acaagatgtg ctggactggt aaacgatggg gatacctgct
     9601 tacgcgagag caataagtgg ctggccacaa ttctcggtac aaaactctgc tgaatagcag
     9661 cgattttccc agcgtggtgc gtcgtttgca ttttactggt tctaagttaa caattatctg
     9721 ttcactaatg actactctaa aaacttgtat caggactatc acttatttaa gtgatattgg
     9781 ttgtctggag attcaggggg cctgtctaga gagatataaa gataccttca ccccggtgat
     9841 gataaaataa aagtatctca gctattaaac tgatgtacga ttcgggcaat aacttattcg
     9901 tatagag
//
LOCUS       DAHHTG010000149         9858 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.152, whole genome shotgun sequence.
ACCESSION   DAHHTG010000149 DAHHTG010000000
VERSION     DAHHTG010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9858)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9858)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9858
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.152"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            229..1323
                     /gene="ddlA"
                     /locus_tag="NBI74_003900"
     CDS             229..1323
                     /gene="ddlA"
                     /locus_tag="NBI74_003900"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HCD1934169.1"
                     /translation="MEKLRVGIVFGGKSAEHEVSLQSAKNIVDAIDKSRFDVVLLGID
                     KQGQWHVSDASNYLLNADDPAHIALRPSATSLAQVPGKHEHQLIDAQNGQPLPTVDVI
                     FPIVHGTLGEDGSLQGMLRVANLPFVGSDVLASAACMDKDVTKRLLRDAGLNIAPFIT
                     LTRANRHNISFAEVESKLGLPLFVKPANQGSSVGVSKVTSEEQYTIAVDLAFEFDHKV
                     IVEQGIKGREIECAVLGNNNPQASTCGEIVLTSDFYAYDTKYIDEDGAKVVVPAAIAP
                     EINDKIRAIAVQAYQTLGCAGMARVDVFLTPENEVVINEINTLPGFTNISMYPKLWQA
                     SGLGYTDLITRLIELALERHAADNALKTTM"
     gene            complement(1347..1559)
                     /locus_tag="NBI74_003901"
     CDS             complement(1347..1559)
                     /locus_tag="NBI74_003901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414914.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2754 domain-containing protein"
                     /protein_id="HCD1934170.1"
                     /translation="MNLPVKIRRDWHYYAFAIGLIFILNGVVGLLGFEAKGWQTYAVG
                     LVTWVISFWLAGLIIRRRDEETENAQ"
     gene            1819..2127
                     /locus_tag="NBI74_003902"
     CDS             1819..2127
                     /locus_tag="NBI74_003902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiY family protein"
                     /protein_id="HCD1934171.1"
                     /translation="MADFTLSKSLFSGKYRNASSTPGNIAYALFVLFCFWAGAQLLNL
                     LVHAPGVYERLMQVQETGRPRVEIGLGVGTIFGLIPFLVGCLIFAVVALWLHWRHRRQ
                     "
     gene            complement(2186..3280)
                     /gene="yaiW"
                     /locus_tag="NBI74_003903"
     CDS             complement(2186..3280)
                     /gene="yaiW"
                     /locus_tag="NBI74_003903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface-exposed outer membrane lipoprotein YaiW"
                     /protein_id="HCD1934172.1"
                     /translation="MSRVNPLSSLSLLAVLVLAGCSSQAPQPLKKGEKAIDVASVVRQ
                     KMPASVKDRDAWAKDLATTFESQGLAPTLENVCSVLAVAQQESNYQADPAVPGLSKIA
                     WQEIDRRAERMHIPAFLVHTALKIKSPNGKSYSERLDSVRTEKQLSAIFDDLISMVPM
                     GQTLFGSLNPVRTGGPMQVSIAFAEQHTKGYPWKMDGTVRQEVFSRRGGLWFGTYHLL
                     NYPASYSAPIYRFADFNAGWYASRNAAFQNAVSKASGVKLALDGDLIRYDSKEPGKTE
                     LATRKLAGKLGMSDSEIRRQLEKGDSFSFEETALYKKVYQLAEAKTGKSLPREMLPGI
                     QLESPKITRNLTTAWFAKRVDERRARCMKQ"
     gene            complement(3293..4513)
                     /gene="sbmA"
                     /locus_tag="NBI74_003904"
     CDS             complement(3293..4513)
                     /gene="sbmA"
                     /locus_tag="NBI74_003904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide antibiotic transporter SbmA"
                     /protein_id="HCD1934173.1"
                     /translation="MFKSFFPKPGMFFLSAFVWALIAVIFWQVGGGDWVARITGASGQ
                     IPISAARFWSLDFLIFYAYYIVCVGLFALFWFIYSPHRWQYWSILGTALIIFVTWFLV
                     EVGVAVNAWYAPFYDLIQTALSSPHKVTIEQFYREVGVFLGIALIAVVISVLNNFFVS
                     HYVFRWRTAMNEYYMANWQQLRHIEGAAQRVQEDTMRFASTLENMGVSFINAIMTLIA
                     FLPVLVTLSAHVPELPIVGHIPYGLVIAAIVWSLMGTGLLAVVGIKLPGLEFKNQRVE
                     AAYRKELVYGEDDATRATPPTVRELFSAVRKNYFRLYFHYMYFNIARILYLQVDNVFG
                     LFLLFPSIVAGTITLGLMTQITNVFGQVRGAFQYLINSWTTLVELMSIYKRLRSFEHE
                     LDGDKIQEVTHTLS"
     gene            4865..6022
                     /gene="ampH"
                     /locus_tag="NBI74_003905"
     CDS             4865..6022
                     /gene="ampH"
                     /locus_tag="NBI74_003905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine-
                     carboxypeptidase/endopeptidase AmpH"
                     /protein_id="HCD1934174.1"
                     /translation="MKRSLLFSAVLYSASLTSVHAAQPITEPEFASDIVDRYADHIFY
                     GSGATGMALVVIDGNQRVFRSYGETRPGNNVRPQLDSVIRIASLTKLMTSEMLVKLLD
                     QGTVKLNDPLSKYAPPGARVPTYNGTPITLVNLATHTSALPREQPGGAAHRPVFVWPT
                     REQRWKYLSTAKLKAAPGSQAAYSNLAFDLLADALANASGKPYTQLFEEQITRPLGMK
                     DTTYTPSPDQCRRLMVAERGASPCNNTLAAIGSGGVYSTPGDMMRWMQQYLSSDFYQR
                     SNQADRMQTLIYQRAQFTKVIGMDVPGKADALGLGWVYMAPKEGRPGIIQKTGGGGGF
                     ITYMAMIPQKNIGAFVVVTRSPLTRFKNMSDGINDLVTELSGNKPLVIPAS"
     gene            complement(6023..6646)
                     /gene="iprA"
                     /locus_tag="NBI74_003906"
     CDS             complement(6023..6646)
                     /gene="iprA"
                     /locus_tag="NBI74_003906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogen peroxide resistance inhibitor IprA"
                     /protein_id="HCD1934175.1"
                     /translation="MLSVVKPLQEFGKLDKCLSRYGTRFEFNNEKQVIFSSDVNSEDT
                     FVILEGVISLRREENVLIGITQAPYIMGLADGLMKNDIPYKLISEGNCTGYHLPAKQT
                     ITLIEQNQLWRDAFYWLAWQNRILELRDVQLIGHNSYEQIRATLLSMIDWNEELRSRI
                     GVMNYIHQRTRISRSVVAEVLAALRKGGYIEMNKGKLVAINRLPSEY"
     gene            complement(6732..9719)
                     /locus_tag="NBI74_003907"
     CDS             complement(6732..9719)
                     /locus_tag="NBI74_003907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000556401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HCD1934176.1"
                     /translation="MHSWKKKLVVSQLALACTLAITSQANAANYDTWGYHDNTTTDLV
                     WPYDTDNDGIADAGGMDPMGSDGNYVTYNGFVYYNNANGDFDTVFKGDTVNGTISTYY
                     LNHDYASGSVNQLDISNSVIHGSITSELPFGYYSITPAEVNAKGEVVTPQTYSGYNFY
                     NGITTDLDYNWYDGDVFTLNVSNSTIDDDYEALYFTDTYLDGDKSKSTNETFYTALGV
                     AVNLDVESNINITNNSRVAGIALSEGNTSNTTYTSEYHQWDNNINVSNSTVTSGSQTP
                     LEESNGFFGKSAEPSDYAGNGGENDVALSFSDNAGSNYSMKNNVNFDHSTLLGDVEFT
                     SHWNNDGVFFYSTGHDSNGDGVLDTNGGWVDDAQNVDELNITLNNGSKWVGSANMSAE
                     VIAPADMYDVAPNSLTPGATIEANDWGRIIDNKVFQSGVFNVALNNGSEWNTVNSSVI
                     DTLAVNNGSQVNVTDSSLVSDTIGLTNGSSLNIGEDGLVATDHLTVDSYSTVNLTEST
                     GWNNYSNLYANTITVTNGGVLDVNVDQFDTEAFRTDKLELTSGNIADHNGNVVSGVFD
                     INSSDYVLNADLVNDRTWDTSKSNYGYGIVAMNSDGHLTINGNGDVDNGTELDNSSVD
                     NVVAATGNYKVRIDNATGAGAIADYKDKEIIYVNDKNSNATFSAANKADLGAYTYQAE
                     QRGNTVVLQQMELTDYANMALSIPSANTNIWNLEQDTVGTRLTNSRHGLADNGGAWVS
                     YFGGNFNGDNGTINYDQDVNGIMVGVDTKIDGNNAKWIVGAAAGFAKGDMNDRSGQVD
                     QDSQTAYIYSSAHFANNVFVDGSLSYSHFNNDLSATMSNGTYVDGSTNSDAWGFGLKA
                     GYDFKLGDAGYVTPYGSVSGLFQSGDDYQLSNNMKVDGQSYDSMRYELGVDAGYTFTY
                     SEDQALTPYFKLAYVYDDSNNDNDVNGDSIDNGTEGSAVRVGLGTQFSFTKNFSAYTD
                     ANYLGGGDVDQDWSANVGVKYTW"
BASE COUNT         2555 a         2446 c         2309 g         2548 t
ORIGIN      
        1 aattaagaga aaaaaactca ccacatggtc atttaagcca ggaataaatt agcttcccgt
       61 tcatcatttt tgtgatcttg cacacatctt tgagataacg cactttcttc accagtacgt
      121 tgctcgcttt gtcagcagaa gattgcaaca ttctgtcaga aggccgcgaa tcccccgttt
      181 tgtcgtggta atgtatgcac tttgtgttag acgggatagg tttttaagat ggaaaaactg
      241 cgggtaggaa tcgtttttgg tggtaaatca gcggaacatg aagtgtctct gcaatcggca
      301 aaaaacattg tcgatgccat tgataaaagt cgcttcgatg ttgtgctgct gggcattgat
      361 aaacaagggc aatggcacgt cagcgatgcc agcaattatc tgctaaatgc agacgatcct
      421 gcccatattg cgttgcgtcc ttcggcgacc agccttgcgc aggtgccagg taaacatgag
      481 catcagctta tcgacgcaca aaacggtcag ccgttgccga cggtggatgt cattttcccg
      541 attgtccacg gtacgctggg cgaagatggt tccttgcagg gaatgctgcg ggtcgccaat
      601 ttaccgtttg taggttctga tgttctggct tcagcggcct gtatggataa agatgtcacc
      661 aaacgtctgc tgcgcgatgc cgggctgaac attgcgccat ttattaccct gacgcgcgct
      721 aatcgtcaca acatcagttt tgccgaagtg gagtctaaac tggggttacc gctgtttgta
      781 aaaccggcta atcagggctc ttctgttggt gtcagcaaag taaccagtga agaacagtac
      841 acaattgccg tcgatctggc gttcgagttc gatcataaag tgatcgttga gcaagggatc
      901 aaaggtcgtg aaatcgaatg cgcagttctt ggcaacaata acccgcaagc aagcacctgt
      961 ggcgagatcg tactcaccag cgatttctat gcctacgaca ccaagtacat tgacgaagat
     1021 ggcgcgaaag tggtagttcc ggcagccatt gcgccagaaa tcaacgataa gatccgggcg
     1081 attgccgttc aggcttatca gacgttggga tgcgcaggca tggcgcgtgt agacgtgttt
     1141 ttaaccccag agaacgaagt ggtgatcaac gagatcaaca ccctgcctgg cttcaccaac
     1201 atcagtatgt atccgaagct gtggcaagcc agcggtctgg gttacaccga cctgatcacg
     1261 cgtttgattg agctggcgct ggagcgtcac gctgcggata acgcactgaa aaccacaatg
     1321 taatattcaa acgcccggta atattcttat tgggcgtttt cagtttcttc atcgcgacga
     1381 cgaataatca atcccgccag ccagaaacta atcacccacg tcaccagacc gacggcatag
     1441 gtctgccaac cttttgcttc aaatcccagt aaccccacca cgccattaag aatgaatata
     1501 aggccaatgg cgaacgcata gtagtgccag tcacggcgga tttttacagg cagattcatg
     1561 gcagctccgt taacgaaaaa caacatcttt tcatattctg taacaactgt ctgtggtgta
     1621 gacaacactt tcgcaattgt taataaattt aacttaaggt tgctattttg tgactcgcaa
     1681 cgaagattca cgcttcggga atattcccaa catcaattta ccatgattct gatattgaca
     1741 atctaaatga cctgtcagac ttgccataag ccgctggtat cacaccagaa gagaagcaga
     1801 tgacttccgg aggtttttat ggctgatttc accctgtcaa aatcgctgtt tagcggaaaa
     1861 tatcgcaatg cctcttcaac gcctggcaat attgcctatg cgttgtttgt gctgttttgc
     1921 ttttgggctg gggcacaatt gctgaacctg ctagtgcatg cgcccggcgt ctatgagcgt
     1981 ttaatgcagg tccaggaaac aggtcgccca cgggtggaaa ttggtttagg tgtcggcacc
     2041 atttttgggc tgatcccgtt tttagtaggc tgcctcattt ttgcagtagt ggcgctatgg
     2101 ctgcactggc gacatcgccg ccagtaagat ttacgttgcc agatgcggcc taaaacgcct
     2161 tatctggcgt gacattcctg tttgatcact gtttcataca acgcgcccgc cgttcgtcta
     2221 cgcgcttcgc aaaccaggcc gtagtcagat tgcgggtgat tttcgggctt tccagttgaa
     2281 tgccaggcaa catttcgcgg ggtagtgatt taccggtttt cgcttcggca agttgataaa
     2341 ctttcttgta cagcgccgtc tcctcaaaag agaagctgtc ccctttctct aactgacggc
     2401 gaatttcgct gtcactcatc cccagttttc ccgccagttt gcgcgttgcc agttcggttt
     2461 tcccgggctc tttactgtcg taacgaatta aatcaccgtc cagtgccagc ttcacgccgc
     2521 tggccttact gaccgcgttc tgaaacgcgg cattacggct ggcgtaccag ccagcgttaa
     2581 aatcagcaaa acggtatatc ggcgcgctat agctggcggg ataattcagt aaatggtaag
     2641 taccaaacca caacccgccg cggcgactga agacttcctg acggactgta ccgtccattt
     2701 tccacggata ccctttggta tgctgttcgg caaaagcaat actgacctgc atcggcccac
     2761 cggtgcgcac cggattgagc gaaccaaaca gcgtctgccc cattggcacc atgctgatca
     2821 gatcgtcaaa aatcgcgctc aattgctttt ctgtacgcac cgaatctaac cgttcgctat
     2881 agcttttgcc gtttggcgat ttgattttta gcgcggtatg caccagaaaa gcaggaatgt
     2941 gcatccgttc agcacgacgg tcaatctctt gccaggcgat cttgcttaag cccggaacgg
     3001 ccggatctgc ctgataattt gactcctgct gcgccaccgc cagcaccgag cagacgtttt
     3061 ccagcgtcgg tgccagcccc tgactttcaa aggtggtggc gagatctttc gcccaggcat
     3121 cgcggtcttt cacgctcgca ggcatcttct gccgcacgac gctcgccaca tctatcgctt
     3181 tttcgccctt tttcaacggt tgcggagcct gactactaca ccccgctaaa accagaaccg
     3241 ccaacaatga cagcgaagat agaggattga cgcgcgacat tacttctcct ttttagctca
     3301 aggtatgggt tacttcctga attttgtcac catccagctc atgttcaaag ctgcgcagac
     3361 gtttgtagat agacatcaac tcaaccagtg tggtccatga gttaatcagg tactggaaag
     3421 caccgcgaac ctgaccaaaa acgttagtaa tctgcgtcat caggccgagt gtaatcgtac
     3481 cggcaacaat tgacggaaac agcaagaaca aaccgaaaac gttatcgacc tgcaaataga
     3541 gaatgcgggc gatattgaaa tacatatagt gaaaatagag gcggaaatag tttttccgta
     3601 cggcgctaaa aagctcgcgt accgtaggcg gcgtcgcgcg cgtggcatcg tcttcaccat
     3661 aaaccagttc tttacggtag gcagcctcta cacgctggtt tttaaactcc agccccggca
     3721 gtttgatccc taccactgcc agtaatccgg tccccatcag cgaccagacg atagcggcaa
     3781 tcaccagacc atacggaatg tgcccgacaa tcggcagctc cggcacatgt gcggagagcg
     3841 ttaccagcac cggcaggaag gcgatcaacg tcatgatagc gttgataaag ctgacgccca
     3901 tattctccag cgttgaagca aaacgcatgg tgtcttcctg cacacgctgc gcggcccctt
     3961 cgatatgacg cagttgttgc cagttcgcca tgtaatattc gttcatcgcc gtacgccagc
     4021 ggaacacgta gtgactgaca aagaagttgt tcagcacact gatcaccacc gcgatcagcg
     4081 caatccccag aaagacgccc acttcgcggt aaaattgttc gatggtgact ttatgcggcg
     4141 aacttagcgc cgtttgaatc agatcataga acggcgcata ccaggcgttg acggcaaccc
     4201 cgacttccac caaaaaccag gtgacgaaga taatcagcgc agtaccgagt attgaccagt
     4261 attgccaacg gtgcgggcta tagataaacc agaacaatgc aaaaagtcct acgcaaacaa
     4321 tgtagtaagc gtaaaaaatc aggaaatcca acgaccagaa acgcgcggcg ctaatcggga
     4381 tctgcccgga agcgccggtg atacgcgcca cccaatcacc cccaccgact tgccagaaga
     4441 taacggcaat caatgcccaa acaaaggccg agagaaaaaa cattcccggc tttgggaaaa
     4501 aagacttaaa catatgcact cctgctaact tcttatcgtt ttaacaactg ctgtgtatta
     4561 accgcatgac cgctacaaag cgtcagccaa atgaaaccgc ccattgtgac ccatagcaag
     4621 gcgcttagtt cgccagcctg accataagaa ttgtttcgac gggtttcaca aaccaaagat
     4681 tgtttacata agcggattaa cacgcgcagg atcacaaatg caggcattgg tgagcaggaa
     4741 aaagatcgtg tttacgcgcc gttacgtttt taacactatt attgcactaa ttcgcccttt
     4801 gcaatccatc aatgagtagt ataaatacgc tcagttacct tcattcaatc tatggacacc
     4861 accgttgaaa cgtagtctgc ttttttctgc cgtgctgtat tcggcgtcat tgacctccgt
     4921 ccatgcggct cagccgatca ctgaaccgga gtttgcctct gatattgtcg atcgttatgc
     4981 cgatcatatt ttttacggca gcggtgccac ggggatggcg ctggttgtta tcgacggtaa
     5041 ccagcgcgtc tttcgcagtt atggcgaaac gcgacctggt aataacgttc gcccgcagct
     5101 ggattccgtc atacgtattg cttccctcac caagttaatg accagtgaga tgctggtaaa
     5161 attgctcgac caggggaccg tgaagttaaa cgatccgcta agtaaatatg ctccgccagg
     5221 cgcacgcgta ccgacctaca atgggacgcc cattacgctg gtgaatctgg caacccatac
     5281 cagcgccctg ccccgtgaac agcccggtgg cgcggcacat cgtccggtat ttgtctggcc
     5341 aacgcgcgag caacgctgga aatacctttc cactgccaaa ctaaaagccg cgccgggcag
     5401 ccaggccgcg tactccaacc tcgcattcga tctgctggca gatgcactgg cgaatgcgtc
     5461 cggcaagcct tatacccagt tgtttgaaga gcagattacc cgtccgttgg ggatgaaaga
     5521 caccacctac accccctcac cggatcagtg ccgccgttta atggttgctg agcgcggtgc
     5581 cagtccgtgc aataacacgc tggcggcaat tggcagcggc ggcgtctatt ccacgccagg
     5641 cgatatgatg cgctggatgc agcagtattt gtcatctgat ttttatcaac gcagtaacca
     5701 ggctgatcgc atgcagacgt tgatttatca gcgcgcgcag tttacaaaag tgattggcat
     5761 ggatgtcccc ggcaaagcgg atgcgcttgg tttaggctgg gtatatatgg ccccgaaaga
     5821 gggtcgtccg gggattattc agaagacagg tggtggtggc ggtttcatta cctatatggc
     5881 gatgatcccg cagaagaata ttggtgcgtt tgtggtggtc actcgttcgc cgttgacgcg
     5941 ctttaaaaat atgagcgatg gcatcaatga cctggtgacc gagctaagcg ggaataaacc
     6001 gttggttatc cccgcatcct gattaatact ctgaaggcaa acggttgata gcgaccagtt
     6061 tgcctttatt catttcgata tagccgcctt tacgcaaagc agcgagaact tccgcaacga
     6121 cagaacgcga tatacgtgta cgttgatgga tataattcat cacgccaata cgtgatcgca
     6181 attcttcatt ccagtcaatc attgataata acgtcgcgcg gatttgttcg taggaattat
     6241 gcccaatgag ctgaacgtcg cgtaattcca gaattctatt ttgccaggct aaccagtaaa
     6301 aagcgtctcg ccagagttga ttttgttcaa taagagtaat ggtttgtttg gctggcaaat
     6361 gatatcccgt acagtttcct tctgatatta atttgtatgg tatatcgttt ttcattaaac
     6421 catcagccag ccccataata taaggagcct gtgtaatacc gataagtacg ttttcttctc
     6481 tacgcagaga gataacacct tctaaaataa cgaaagtatc ttcgctattg acatcactgg
     6541 aaaatataac ttgcttttca ttattaaact cgaagcgcgt accgtatctg gacaaacatt
     6601 tatcgagctt accaaattcc tgaagaggtt taactacaga taacatttgc gcgtcctttg
     6661 cagtaatgcc cgtcaaatcc ttgacgggca ttatttagat taaattacct atttcttcgg
     6721 agtgaagaat attaccaggt atatttaaca cccacattcg cggaccagtc ttgatctacg
     6781 tcaccaccac cgaggtagtt agcatcggta taggcgctga agttcttggt gaagctgaac
     6841 tgagtaccca gaccaacacg taccgcagac ccttcagtac cgttatcgat ggaatcaccg
     6901 ttcacatcgt tatcgttgtt agagtcgtcg tagacgtaag ccagtttgaa gtacggagtc
     6961 agagcctggt cttcgctgta ggtgaaggta taacctgcat ctacacccag ttcataacgc
     7021 atgctgtcgt aagactgacc atcaactttc atgttgttgc tcagctggta atcatcacca
     7081 gactggaaca gaccagaaac gctgccgtaa ggagttacgt aacctgcatc acccagtttg
     7141 aagtcgtaac cggctttcaa accaaagccc caagcgtcgg agttggtgct gccgtcaacg
     7201 taagtaccgt tgctcatggt tgcagacagg tcgttgttga agtgagagta gctcaagcta
     7261 ccgtcaacaa agacgttgtt cgcgaagtga gcagaagagt agatgtaggc agtctggctg
     7321 tcttgatcca cctgaccaga acggtcattc atatcacctt tagcgaagcc tgcagccgca
     7381 ccgacgatcc acttagcgtt gttaccgtca attttggtat caacaccgac catgatgccg
     7441 ttaacatcct gatcatagtt gatggtgccg ttgtcgccgt tgaagttacc accgaagtag
     7501 cttacccatg cgccgccgtt atcagccaga ccatgacgag agttggtcag acgagtacca
     7561 acggtgtctt gttccaggtt ccagatattg gtgttcgcag acgggatgct cagcgccatg
     7621 ttagcgtagt cggtcagctc catctgttgc agaacaacgg tgttaccacg ctgctcagcc
     7681 tgataggtgt atgcacccag gtcagctttg ttagcagcag agaaagtcgc gttgctgttt
     7741 ttgtcgttga cgtagataat ttctttatct ttgtaatcag cgattgcgcc agcgccagtt
     7801 gcgttgtcga tacgaacttt gtagttaccg gttgcagcaa caacgttgtc tacagaactg
     7861 ttatccagtt cagtaccgtt gtctacgtcg ccgttgccat tgatagtcag gtgaccatca
     7921 gagttcatag caacaatacc gtaaccgtag ttagacttgg aagtatccca cgtacggtcg
     7981 ttcaccaaat ccgcgttcag aacgtaatcg ctgctgttga tgtcgaacac accagaaact
     8041 acgttgccgt tatggtcagc gatgttgccg ctagtcagtt ccagtttgtc agtacggaaa
     8101 gcttcagtat caaactgatc aacgttcaca tccagtacac caccgttagt tacggtgatg
     8161 gtattggcat acaggttaga atagttattc cagccagtag attcggttaa attaacggta
     8221 ctgtagctat caacagtcag gtggtctgta gcaaccaaac catcttcacc aatattcagt
     8281 gaggagccgt tggttaaacc aatggtatct gagaccagag aggaatcagt aacatttacc
     8341 tgagagccat tatttacagc taatgtatca attactgaac tattaacggt attccattct
     8401 gaaccattgt tcagtgctac gttaaatacg ccgctctgga ataccttgtt gtcaataata
     8461 cgaccccaat cgttagcttc gatagtggca ccaggggtca ggctgttcgg cgcaacatca
     8521 tacatatctg ccggagcaat tacttcagca ctcatgtttg ctgagccaac ccacttacta
     8581 ccgttgttca gagtaatgtt cagttcatca acgttctgag catcatctac ccaaccaccg
     8641 ttggtatcaa ggacaccgtc accattgctg tcatggccgg ttgaatagaa gaatacgcca
     8701 tcgttattcc agtggcttgt gaactcgaca tcacccaaca gggttgaatg gtcaaagttt
     8761 acattgtttt tcatggaata atttgagcca gcattgtcag agaatgacag agctacgtca
     8821 ttttcgccac cattaccagc gtaatcactt ggttcagcag atttaccgaa gaagccattg
     8881 ctttcttcca gcggagtctg tgaccctgaa gttacagtgg agttagacac attaatgttg
     8941 ttgtcccact gatgatattc gctagtataa gtcgtgttgc ttgtattgcc ttcagacagc
     9001 gcaataccag ctacacggga gttgttagta atattgatat tgctttcaac atcaagattt
     9061 actgcaacac ccagagcggt atagaacgtt tcattggttg acttagattt atcaccatca
     9121 agatatgtgt cagtgaagta aagagcttca tagtcatcat caatggtgga attggatacg
     9181 ttcagagtga atacgtcacc atcgtaccaa ttgtagtcaa ggtcagtagt gattccgtta
     9241 tagaaattat agccactata ggtctgtgga gttacaactt cacctttagc attaacttct
     9301 gcaggagtaa tgctgtaata accaaatggc agttcagaag tgattgaccc atgaattact
     9361 gaattgctga tatctaactg gttaacagaa ccgctagcgt aatcatgatt caagtaataa
     9421 gtagaaatgg taccgttaac agtatcgcct ttaaagacgg tgtcgaaatc accattggca
     9481 ttgttatagt aaacaaaacc attgtaagtt acgtagttac cgtctgaacc catcgggtcc
     9541 atgccgccag catcggcaat tccatcattg tcagtgtcat acggccaaac taaatcggtc
     9601 gtggtgttgt catgataccc ccaggtatca tagtttgctg cattagcctg agaggtgata
     9661 gccagagtgc aagccaatgc taattgtgat actacaagtt tctttttcca ggagtgcatt
     9721 tgtcatccct cctcagggac gtaataagtt gatccatcaa ttatcaatgc atagaaaaca
     9781 tattatgttt cgcgtttacg attatgcggc gttatagagg aatggttcaa ttattctttc
     9841 ccctaagtcc gattgcgg
//
LOCUS       DAHHTG010000150         9835 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.153, whole genome shotgun sequence.
ACCESSION   DAHHTG010000150 DAHHTG010000000
VERSION     DAHHTG010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9835)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9835)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9835
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.153"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            211..1368
                     /gene="acrE"
                     /locus_tag="NBI74_003908"
     CDS             211..1368
                     /gene="acrE"
                     /locus_tag="NBI74_003908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrE"
                     /protein_id="HCD1934177.1"
                     /translation="MTKHARFFLLPSFILISAALIAGCNDKGEEKAHVGEPQVTVHIV
                     KTAPLEVKTELPGRTNAYRIAEVRPQVSGIVLNRNFTEGSDVQAGQSLYQIDPATYQA
                     SYDSAKGELAKSEAAAAIAHLTVKRYVPLVGTKYISQQEYDQAIADARQADAAVIAAK
                     ATVESARINLAYTKVTAPISGRIGKSTVTEGALVTNGQTTELATVQQLDPIYVDVTQS
                     SNDFMRLKQSVEQGNLHKENATSNVELVMENGQTYPLKGTLQFSDVTVDESTGSITLR
                     AVFPNPQHTLLPGMFVRARIDEGVQPDAILIPQQGVSRTPRGDATVLIVNNKSQVEAR
                     PVVASQAIGDKWLISEGLKSGDQVIVSGLQKARPGEQVKATTDTPADTASK"
     gene            1380..4484
                     /gene="acrF"
                     /locus_tag="NBI74_003909"
     CDS             1380..4484
                     /gene="acrF"
                     /locus_tag="NBI74_003909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit AcrF"
                     /protein_id="HCD1934178.1"
                     /translation="MANFFIRRPIFAWVLAIILMMAGALAILQLPVAQYPTIAPPAVS
                     VSANYPGADAQTVQDTVTQVIEQNMNGIDNLMYMSSTSDSAGSVTITLTFQSGTDPDI
                     AQVQVQNKLQLATPLLPQEVQQQGISVEKSSSSYLMVAGFVSDNPGTTQDDISDYVAS
                     NVKDTLSRLNGVGDVQLFGAQYAMRIWLDADLLNKYKLTPVDVINQLKVQNDQIAAGQ
                     LGGTPALPGQQLNASIIAQTRLKNPEEFGKVTLRVNSDGSVVRLKDVARVELGGENYN
                     VIARINGKPAAGLGIKLATGANALDTAKAIKAKLAELQPFFPQGMKVLYPYDTTPFVQ
                     LSIQEVVKTLFEAIMLVFLVMYLFLQNMRATLIPTIAVPVVLLGTFAILAAFGYSINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVMMEDKLPPREATEKSMSQIQGALVGIAMVLS
                     AVFIPMAFFGGSTGAIYRQFSITIVSAMALSVLVALILTPALCATLLKPVSAEHHENK
                     GGFFGWFNTTFDHSVNHYTNSVGKILGSTGRYLLIYALIVAGMVVLFLRLPSSFLPEE
                     DQGVFLTMIQLPAGATQERTQKVLDQVTDYYLKNEKANVESVFTVNGFSFSGQEQNAG
                     MAFVSLKPWEERSGDENSAEAVIHRAKMELGKIRDGFVIPFNMPAIVELGTATGFDFE
                     LIDQAGLGHDALTQARNQLLGMAAQHPASLVSVRPNGLEDTAQFKLEVDQEKAQALGV
                     SLSDINQTISTALGGTYVNDFIDRGRVKKVYVQADAKFRMLPEDVDKLYVRSANGEMV
                     PFSAFTTSHWVYGSPRLERYNGLPSMEIQGEAAPGTSSGDAMALMENLASKLPAGIGY
                     DWTGMSYQERLSGNQAPALVAISFVVVFLCLAALYESWSIPVSVMLVVPLGIVGVLLA
                     ATLFNQKNDVYFMVGLLTTIGLSAKNAILIVEFAKDLMEKEGKGVVEATLMAVRMRLR
                     PILMTSLAFILGVLPLAISNGAGSGAQNAVGIGVMGGMVSATLLAIFFVPVFFVVIRR
                     CFKG"
     gene            4737..4958
                     /locus_tag="NBI74_003910"
     CDS             4737..4958
                     /locus_tag="NBI74_003910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1934179.1"
                     /translation="MKRLIPVALLTALLAGCAHDSPCVPVYDDQGRLVHTNTCMKGTT
                     QDNWETAGAIAGGAAAVAGLTMGIIALSK"
     gene            5389..6414
                     /locus_tag="NBI74_003911"
     CDS             5389..6414
                     /locus_tag="NBI74_003911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HCD1934180.1"
                     /translation="MKKMMIATLAAASVLLAVANQAHAGATLDAVQKKGFVQCGISDG
                     LPGFSYADADGKFSGIDVDVCRGVAAAVFGDDTKVKYTPLTAKERFTALQSGEVDLLS
                     RNTTWTSSRDAGMGMAFTGVTYYDGIGFLTHDKAGLKSAKELDGATVCIQAGTDTELN
                     VADYFKANNMKYTPVTFDRSDESAKALESGRCDTLASDQSQLYALRIKLSNPTEWIVL
                     PEVISKEPLGPVVRRGDDEWFSIVRWTLFAMLNAEEMGINSQNVDEKAANPATPDMAH
                     LLGKEGDYGKDLKLDNKWAYNIIKQVGNYSEIFERNVGSESPLKIKRGQNNLWNNGGI
                     QYAPPVR"
     gene            6482..7663
                     /locus_tag="NBI74_003912"
     CDS             6482..7663
                     /locus_tag="NBI74_003912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HCD1934181.1"
                     /translation="MPHRRSTVKGSLSFANPTVRAWLFQILAVVAAVSIVGWLFHNTV
                     TNLSNRGITSGFAFLDRGAGFGIVQHLIDYQQGDTYGRIFIVGLLNTLLVSALCIVFA
                     SVLGFFIGLARLSDNWLLRKLSTIYIEIFRNIPPLLQIFFWYFAVLRNLPGPRQAVSA
                     FDLAFLSNRGLYIPSPQLGDGFIAFILAVVMAIVLSVGLFRFNKTYQIKTGQLRRTWP
                     FAAVLIIGLPLLAQWLFGAALHWDVPALRGFNFRGGMVLIPELAALTLALSIYTSAFI
                     AEIIRAGIQAVPYGQHEAARSLGLPNPVTLRQVIIPQALRVIIPPLTSQYLNIVKNSS
                     LAAAIGYPDMVSLFAGTVLNQTGQAIETIAMTMSVYLIISLTISLLMNIYNRRIAIVE
                     R"
     gene            7673..8776
                     /locus_tag="NBI74_003913"
     CDS             7673..8776
                     /locus_tag="NBI74_003913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HCD1934182.1"
                     /translation="MTKVLLSHPPRPASHNSSRAMVWVRKNLFSSWSNSLLTIGCIWL
                     MWELIPPLLNWAFLQANWVGSTRADCTKAGACWVFIHERFGQFMYGLYPHDQRWRINL
                     ALLIGLVSIAPMFWKILPHRGRYIAVWAVIYPLVVWWLMYGGFLGLDRVETRQWGGLT
                     LTLIIASVGIAGALPWGILLALGRRSHMPIVRILSVIFIEFWRGVPLITVLFMSSVML
                     PLFMAEGTSIDKLIRALVGVILFQSAYVAEVVRGGLQALPKGQYEAAESLALGYWKTQ
                     GLVILPQALKLVIPGLVNTIIALFKDTSLVIIIGLFDLFSSVQQATVDPAWLGMSTEG
                     YVFAALIYWIFCFSMSRYSQHLEKRFNTGRTPH"
     gene            8784..9542
                     /locus_tag="NBI74_003914"
     CDS             8784..9542
                     /locus_tag="NBI74_003914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HCD1934183.1"
                     /translation="MSQILLQPANAMITLENVNKWYGQFHVLKNINLTVQPGERIVLC
                     GPSGSGKSTTIRCINHLEEHQQGRIVVDGIELNEDIRNIERVRQEVGMVFQHFNLFPH
                     LTVLQNCTLAPIWVRKMPKKEAEALAMHYLERVRIAEHAQKFPGQISGGQQQRVAIAR
                     SLCMKPKIMLFDEPTSALDPEMVKEVLDTMIGLAQSGMTMLCVTHEMGFARTVADRVI
                     FMDRGEIVEQAAPDEFFAHPKSERTRAFLSQVIH"
     gene            complement(9605..9720)
                     /gene="rrf"
                     /locus_tag="NBI74_003915"
     rRNA            complement(9605..9720)
                     /gene="rrf"
                     /locus_tag="NBI74_003915"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(9760..9835)
                     /locus_tag="NBI74_003916"
     tRNA            complement(9760..9835)
                     /locus_tag="NBI74_003916"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9800..9802),aa:Thr,seq:ggt)
BASE COUNT         2345 a         2351 c         2520 g         2619 t
ORIGIN      
        1 aattctattt ctttcattcc gcgtcaaaac aaaacagtag aatattaatc tttttttgtg
       61 tttatgtgcc ttgagttgcc agtattcata actattcctt acatcgacga atgataattt
      121 gtaggatagc gaactgtatt tttctttctg cgagttaacg cattgccttt ttgggcaaat
      181 aacgcgcttt tggttttttg aggaatagta atgacgaaac atgccaggtt tttcctcctg
      241 ccctccttta ttctgatctc cgcggcttta atcgccggtt gtaacgataa gggagaagag
      301 aaagctcacg tcggtgaacc gcaggttacc gttcatattg tgaaaacggc cccgttagaa
      361 gttaagactg aattaccagg ccgcaccaat gcttaccgta tagccgaagt tcgcccacag
      421 gttagcggga tcgtactgaa tcgcaatttc actgaaggca gcgatgtgca agcaggccag
      481 tccctgtacc agatcgatcc cgcgacctat caggcaagtt atgacagcgc gaaaggcgaa
      541 ctggcgaaaa gtgaagccgc cgccgccatc gcgcatttga cggtaaaacg ttacgttccg
      601 ctcgtgggta cgaaatacat cagtcagcag gagtacgacc aggccattgc tgatgctcgc
      661 caggccgatg ccgccgtgat tgccgcaaaa gccacagtcg aaagcgctcg catcaatctc
      721 gcttatacca aagtgactgc gccaattagc ggacgtatcg gcaaatcgac cgtgaccgaa
      781 ggcgctcttg tcactaatgg gcaaacgact gaactggcga ctgtccagca gctcgatcct
      841 atctacgttg atgtgaccca atccagcaac gattttatga ggctgaagca atccgtagag
      901 caaggaaatt tgcataagga aaacgccacc agcaacgtag agttggtcat ggagaacggt
      961 caaacctatc ccctgaaagg tacgctgcaa ttctccgatg tgaccgttga tgaaagcacc
     1021 ggctccataa ccctacgtgc tgtcttccct aacccgcaac atacgctttt gccgggtatg
     1081 tttgtgcgcg cacggattga tgaaggcgtc caacctgacg ccattcttat cccgcaacaa
     1141 ggcgttagcc gcacaccacg tggtgatgca accgtgctga ttgttaacaa taaaagtcag
     1201 gttgaagcgc gccctgtcgt tgccagtcag gcgattggcg ataaatggtt gattagcgaa
     1261 ggtctgaaat ctggcgatca agtcattgtc agcggcctgc aaaaagcgcg tccgggagag
     1321 caggttaaag ccactaccga tacccccgca gatactgcat cgaagtaagg caatctgaca
     1381 tggcaaactt ttttattcga cgaccgatat ttgcatgggt gctggccatt attctgatga
     1441 tggcgggcgc actggcgatc ctacaattgc ccgtcgccca gtatccaacc attgcgccgc
     1501 ctgcggtttc tgtttcggca aactatccgg gtgctgatgc gcagaccgtg caggatacag
     1561 tgacgcaggt tatcgaacag aatatgaacg gtatcgataa cctgatgtat atgtcctcca
     1621 ccagcgattc cgccggtagc gtgacaatta cccttacctt ccagtccggg accgatcctg
     1681 atatcgcgca agtacaggtg cagaacaaac tccagctcgc cacgccgttg ctgccgcagg
     1741 aggttcagca gcaggggatc agtgttgaaa agtccagtag cagctatttg atggtggcgg
     1801 gctttgtctc tgataaccct ggcaccacac aggacgatat ctcggactat gtggcctcta
     1861 acgttaaaga tacgcttagc cgtctgaatg gcgtcggtga cgtacagctt ttcggcgcac
     1921 agtatgcgat gcgtatctgg ctggacgccg atctgctaaa caaatataaa ctgacaccgg
     1981 ttgatgtgat taaccagttg aaggtacaga acgatcagat cgctgccgga cagttgggcg
     2041 gaacgccagc gttaccaggg caacagttga acgcctcgat tattgctcag acgcggttaa
     2101 aaaatccgga agaattcggc aaagtgaccc tgcgcgtaaa cagtgacggc tcggtggtgc
     2161 gtctgaaaga tgtcgcacgg gttgaacttg gcggtgaaaa ctataacgtt atcgctcgca
     2221 tcaacggaaa accggcggcg ggcctgggga ttaagctggc aaccggcgcg aatgctctcg
     2281 ataccgcgaa agccattaag gcaaaactgg cggaattaca gccattcttc ccgcagggaa
     2341 tgaaggttct ttacccttat gacaccacgc ctttcgtcca gctttctatc caggaagtgg
     2401 tgaaaacgct gttcgaagcc attatgctgg tgttcctggt gatgtatctg ttcttgcaga
     2461 acatgcgggc aacattgata cccaccattg ctgtacctgt cgtgttgtta gggacatttg
     2521 ccatactcgc cgcttttggt tactccatca atacgctaac gatgtttggg atggtacttg
     2581 ccatcggact tcttgttgat gatgcgatag tcgttgtgga gaacgtcgag cgcgtaatga
     2641 tggaggataa actccccccc agagaagcga cggaaaaatc gatgtcgcaa atccagggcg
     2701 cgctggtagg tatcgcgatg gtactgtcgg cggtatttat tccgatggca ttcttcggtg
     2761 gctcaacagg ggcaatttat cgccagttct ctatcactat tgtttcagca atggcgcttt
     2821 cagtactggt tgcgctgatt ctcacccccg cgttatgtgc aacattgctg aaaccagtct
     2881 ctgccgagca tcacgaaaat aagggaggat tcttcggctg gtttaacacc actttcgatc
     2941 atagcgttaa tcactacacc aatagcgttg gcaaaatcct cggttccaca gggcgatatt
     3001 tactgatcta tgcgctgatt gttgcaggaa tggtggtgtt gtttttacgt cttccgtctt
     3061 ccttcttacc tgaagaggat cagggtgtct ttctgaccat gattcagtta cccgctggcg
     3121 cgacgcaaga gcggacgcaa aaagtgttgg atcaagtgac ggattactat ctgaagaacg
     3181 agaaagcgaa cgttgaaagt gtctttacag ttaacggctt tagcttcagc ggccaggaac
     3241 aaaacgccgg tatggccttc gtcagtctga aaccgtggga agagcgtagt ggtgacgaaa
     3301 acagtgcgga agcagtaatc catcgtgcca aaatggaatt gggcaagatc cgcgacggtt
     3361 ttgtcattcc atttaatatg ccagccattg ttgaactagg cacggcaacg ggtttcgact
     3421 ttgagttaat tgatcaggct gggctgggtc acgatgccct gacccaggcc cgtaaccagt
     3481 tgcttggcat ggcggcgcaa catcctgcca gcttagtcag cgtgcgccct aatggcctgg
     3541 aagacaccgc gcagtttaaa ctggaagttg accaggaaaa ggcgcaggca ttaggtgttt
     3601 cactttctga catcaatcag accatttcaa cggcgttggg tgggacttac gttaacgact
     3661 tcatcgaccg tggtcgcgtg aaaaaggtgt atgttcaggc ggatgccaaa ttccgtatgc
     3721 tgccagaaga tgtcgataaa ctttatgtcc gcagcgccaa cggcgaaatg gtgccattct
     3781 ctgcctttac cacttcacat tgggtgtatg gctctccgcg actggaacgc tacaacggtc
     3841 tgccgtcaat ggagattcag ggggaagccg cgccaggaac cagttccggc gatgctatgg
     3901 cgttgatgga aaaccttgcg tcaaaattac cggcgggcat tggttatgac tggacgggta
     3961 tgtcatatca ggaacgttta tcgggaaacc aggctcccgc tctggtagca atttcctttg
     4021 tggttgtttt cctgtgcctt gctgcactct atgaaagctg gtcaattcct gtctcggtta
     4081 tgttggtcgt gccgttaggg attgtcggcg tgctactggc ggcaacactc tttaatcaaa
     4141 aaaatgacgt ctactttatg gtgggcttgc taacgacaat tggcttgtcg gccaaaaacg
     4201 ctattttgat tgttgagttc gctaaagatc tcatggagaa agagggtaaa ggtgttgttg
     4261 aagcgacact aatggcagta cgtatgcgcc tgcgtcctat cctgatgacc tctctcgcct
     4321 ttattctcgg cgtattaccg ctagctatca gtaacggtgc cggcagtggc gcgcagaacg
     4381 cagtgggtat cggggtaatg ggcgggatgg tttctgcaac gttgctggca atcttcttcg
     4441 tgccggtgtt ctttgtggtg atccgccgtt gctttaaagg ataaatcaga aacataaagg
     4501 cactttcggg tgcctttatt atttccagtg aaacccataa aaattaataa gatattcctc
     4561 tactcacttt ttaaaaactt tctatagttc ccgctccctt cactattttt acaattcaca
     4621 taatttgtca ttattcattc cgcaagattt ataacctcca gtgataaaat ggcattcagc
     4681 tcgttaataa gagagttaac ttattaagcg ttagcgttta ttactgaggt aacaccatga
     4741 aaagattaat tcctgtggca ttgctcaccg cattgctggc gggctgtgct cacgattcgc
     4801 cctgcgttcc ggtttatgac gatcagggtc gactggttca cactaatacc tgtatgaaag
     4861 gcacgactca ggataactgg gaaacggctg gggctattgc cggcggggca gcagctgttg
     4921 ctggcctgac gatggggatc atcgctctgt cgaaataact aaacagggcg cggagctccg
     4981 cgctcttttt aacgatacag tatctttcca taagataaaa tattgctata ttctgctggg
     5041 taattccctg ctgatttagc atgttatatc acgttatttc gctgctttcg aaataacgca
     5101 tcaatttaac tttcacactt cccaccagaa atatactcac gctaacaatc attattctgc
     5161 ttagtaaaaa acgcgtaaat attttgcgcc aaaatgtggc gcatgtttca ttttcgcacc
     5221 attacgaggc gctgttttta ttatttcctg tctacactca gcttattgcg acgcgaaatc
     5281 gtgcgcaaaa gctggcacta cttttgctta taagaggatg gccacagaca ggtaaaagac
     5341 gtttcccaaa cgtcctataa cgataatttt cgcctcacag gaagcattat gaaaaagatg
     5401 atgatagcca cactggctgc cgccagcgtg ctgcttgccg ttgcaaatca ggcgcatgct
     5461 ggcgcgacgc ttgatgccgt tcagaaaaaa ggttttgtgc aatgcgggat cagtgatgga
     5521 ttacctgggt tctcttatgc cgatgctgac ggtaagtttt caggtattga tgtagatgtt
     5581 tgtcgtggtg ttgccgctgc tgtattcggt gacgatacga aagtgaaata taccccgctc
     5641 actgcaaaag aacgcttcac cgctttacag tcaggggagg tggatttgct ctcccgtaat
     5701 acgacctgga cttcatctcg cgatgccggg atgggaatgg catttactgg cgtcacttat
     5761 tacgacggca ttggcttcct gacgcacgat aaagcggggc taaaaagcgc gaaagaactg
     5821 gatggcgcta ccgtctgtat tcaggcgggg actgataccg aactcaacgt cgccgattac
     5881 ttcaaggcaa ataatatgaa gtacacgcca gtaaccttcg atcgctcaga cgaatcagcg
     5941 aaggctctgg aatctggccg ctgtgatact ctggcctcgg atcaatcaca actgtatgcc
     6001 ctacgcatca aattaagcaa cccaactgaa tggattgtct taccggaagt tatctctaaa
     6061 gaaccacttg ggccggtagt tcgtcgtggc gatgatgaat ggttctcgat tgtacgctgg
     6121 acgcttttcg ccatgctgaa tgctgaagag atgggcatca attcccagaa tgtcgatgaa
     6181 aaagcggcta atccagcaac gcctgatatg gcacatctgc tgggtaaaga aggcgattac
     6241 ggcaaggatc tgaagctgga taataaatgg gcctataaca tcatcaaaca ggtgggtaac
     6301 tactcggaaa ttttcgagcg taacgtaggt tcagaaagcc cgctgaaaat taaacgtggg
     6361 caaaataatc tctggaataa cggcggtatt cagtacgcgc cgcccgtgcg ttaagtcggt
     6421 gtgaggtaac gagcgttgct acggcggcgc tcttttcagc gttatggttt tcgaggtttc
     6481 tatgccccat cgccgctcaa ccgttaaagg ctcactctct tttgccaacc ctacggttcg
     6541 cgcctggtta ttccagatcc ttgccgttgt tgctgctgtc agcattgttg gttggttatt
     6601 tcacaacact gtaacgaatc tcagtaatcg tggcataaca tcaggatttg cttttcttga
     6661 ccgcggggcc ggttttggca tcgttcagca tttgattgat tatcagcaag gcgacactta
     6721 cggacgcatt tttattgtcg gcctactcaa tacgctactg gtttctgcat tgtgtattgt
     6781 gttcgcttct gttttgggct tctttatcgg tctggcgaga ctttcggata actggctgct
     6841 acgaaagctt tccacaattt atattgagat cttccgtaat attcccccgc tgctgcaaat
     6901 cttcttctgg tactttgccg tgttgcgcaa tttgcccgga ccacgacagg cagtgagcgc
     6961 gtttgacctg gcctttttga gcaatcgtgg gctttatatt ccgtcgccgc agctgggaga
     7021 cggatttatt gcgtttatcc tggctgttgt tatggctata gtcctttctg ttgggctatt
     7081 ccgctttaat aaaacatacc agataaagac cggacaactg cgccgcacct ggccattcgc
     7141 cgctgtgttg atcattggtt tgcctttact ggcgcaatgg ctttttggcg cagcactaca
     7201 ctgggatgtc ccagcccttc gaggctttaa tttccgcggc gggatggttt taattcctga
     7261 actggcagcc ttaacgctag cactttcgat ttatacctct gcatttatcg ccgagattat
     7321 ccgcgcaggg atccaggcag tgccttatgg tcaacatgaa gcggctcgat cactgggatt
     7381 acccaacccg gttacgctac gccaggtcat tattccccag gcattgcggg tgattattcc
     7441 accgttaacc agccagtatc tcaacatcgt caagaactcc tctcttgccg ccgctattgg
     7501 ctatcccgat atggtttcgc tgtttgctgg caccgtgctg aaccaaacgg ggcaagccat
     7561 cgagacgata gccatgacca tgtcggtcta tctgattatc agcctgacta tctcgctgct
     7621 gatgaatatc tataaccgcc gcatcgcgat cgttgaacgc taaggagtca tgatgacaaa
     7681 agtattgctg tctcatcccc cgcgcccggc gagccataac tcaagccgcg cgatggtgtg
     7741 ggtgcgaaaa aatctgttct ccagctggag caatagcctg ctgactattg gctgcatatg
     7801 gttgatgtgg gaactgatcc caccgttgct gaactgggca tttttgcagg ctaactgggt
     7861 tggctcaacg cgtgccgact gcacaaaagc cggtgcctgt tgggtcttca tccacgaacg
     7921 atttggtcag tttatgtatg gactttaccc acacgaccaa cgctggcgaa ttaacctggc
     7981 attactgatt ggccttgtgt ctatcgcacc aatgttctgg aaaatacttc cgcatcgcgg
     8041 tcgctatatt gcggtctggg cggtgattta cccactggtt gtctggtggc tgatgtatgg
     8101 tgggtttctt ggtcttgatc gggttgaaac ccggcaatgg ggcgggttga cgctaacttt
     8161 aattattgca tcagttggga ttgcgggggc gctaccgtgg gggatcttac tggcgttagg
     8221 tcgccgctcc catatgccga ttgtgcgtat cttatcggtc atttttatcg agttctggcg
     8281 cggcgtaccg ctgattaccg tattgtttat gtcttcggtc atgctgccgt tgtttatggc
     8341 agaaggcacc agtatcgata aactgatccg cgcgctggtt ggcgtgatcc tgtttcagtc
     8401 agcatatgtt gcggaagtcg tgcgaggcgg attacaggcg ctgcctaaag ggcaatatga
     8461 agcggcagag tcgctggcat tgggttattg gaaaactcag gggcttgtta ttctgcccca
     8521 ggcgttgaag ctggtaattc ctgggctggt aaataccatc atcgcactct tcaaagatac
     8581 cagtctggtg atcatcatcg ggttgttcga tcttttcagt agcgttcagc aggcaaccgt
     8641 tgatcccgcg tggttgggta tgtcgacgga agggtatgtt ttcgccgcgc tgatctactg
     8701 gatcttctgt ttcagcatgt cacgttatag ccaacatctg gaaaaacgtt ttaacaccgg
     8761 gcgtacaccg cattgaggac actatgagcc aaattttact gcaacctgct aacgcgatga
     8821 ttacgctgga aaacgtcaat aaatggtatg gacaattcca tgttttgaaa aatataaatt
     8881 taaccgtgca accgggcgaa cggatcgttc tatgtggccc ttcaggttcc ggtaaatcaa
     8941 caaccattcg ttgtattaat catctggaag aacatcaaca gggacggatc gtggtagatg
     9001 gcatcgaact taatgaagat atccgcaata ttgagcgcgt caggcaggaa gtgggaatgg
     9061 tctttcagca tttcaatctc ttccctcatc tgaccgtgtt acagaactgt accctggcac
     9121 cgatttgggt acgcaagatg ccaaagaaag aggctgaagc tctggcgatg cattaccttg
     9181 agcgggtgag aattgccgaa catgcccaga agtttcccgg acagatttct ggtggtcagc
     9241 agcaacgtgt tgccatagca cgttcgctgt gtatgaagcc gaaaattatg ttgtttgatg
     9301 agccaacgtc ggcgctcgat cctgagatgg tgaaagaggt gctggatacg atgattgggc
     9361 tggcgcagtc gggtatgaca atgttgtgtg taacacatga gatggggttt gcacgaaccg
     9421 tcgctgaccg ggtgattttt atggatcgtg gggaaatagt ggagcaagct gcacctgatg
     9481 aattttttgc gcatcctaaa tcagagcgta cgagggcatt tttatcgcag gtaatccatt
     9541 aattgaatat tagttcgaaa agcaaaaagg ccatcctttc ggatggcctt tcgcctgatt
     9601 tgatgtctgg cagttcccta ctctcgcatg gggagacccc acactaccat cggcgctacg
     9661 gcgtttcact tctgagttcg gcatggggtc aggtgggacc accgcgctac tgccgccaga
     9721 caaattcttt tctaatctgc cgaactttaa cctaaaaagt ggtgctgata cccagagtcg
     9781 aactggggac ctcaccctta ccaagggtgc gctctaccaa ctgagccata tcagc
//
LOCUS       DAHHTG010000151         9798 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.154, whole genome shotgun sequence.
ACCESSION   DAHHTG010000151 DAHHTG010000000
VERSION     DAHHTG010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9798)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9798)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9798
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.154"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            136..1737
                     /gene="aceB"
                     /locus_tag="NBI74_003917"
     CDS             136..1737
                     /gene="aceB"
                     /locus_tag="NBI74_003917"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase A"
                     /protein_id="HCD1934184.1"
                     /translation="MTEQATTIDELAFTRPYGEQEKQILTAEAVEFLTELVTHFTPQR
                     NKLLAARIQQQQDIDNGRLPDFISETASIRDADWKIRGIPADLEDRRVEITGPVERKM
                     VINALNANVKVFMADFEDSLAPDWNKVIDGQINLRDAVNGTISYTNEAGKIYQLKPNP
                     AVLICRVRGLHLPEKHVTWRGEAIPGSLFDFALYFFHNYQALLAKGSGPYFYLPKTQS
                     WQEAAWWSEVFSYAEDRFNLPRGTIKATLLIETLPAVFQMDEILHALRDHIVGLNCGR
                     WDYIFSYIKTLKNYPDRVLPDRQAVTMDKPFLNAYSRLLIKTCHKRGAFAMGGMAAFI
                     PSKDEERNNQVLDKVKADKSLEANNGHDGTWIAHPGLADTAMAVFNDILGSRKNQLEV
                     MREQDAPITADQLLAPCDGERTEEGMRANIRVAVQYIEAWISGNGCVPIYGLMEDAAT
                     AEISRTSIWQWIHHQKTLSNGKPVTKALFRQMLGEEMKVIASELGEERFSQGRFDDAA
                     RLMEQITTSDELIDFLTLPGYRLLA"
     gene            1767..3071
                     /gene="aceA"
                     /locus_tag="NBI74_003918"
     CDS             1767..3071
                     /gene="aceA"
                     /locus_tag="NBI74_003918"
                     /EC_number="4.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase"
                     /protein_id="HCD1934185.1"
                     /translation="MKTRTQQIEELQKEWTQPRWEGITRPYSAEDVVKLRGSVNPECT
                     LAQLGAAKMWRLLHGESKKGYINSLGALTGGQALQQAKAGIEAVYLSGWQVAADANLA
                     ASMYPDQSLYPANSVPAVVERINNTFRRADQIQWSAGIEPGDPRYVDYFLPIVADAEA
                     GFGGVLNAFELMKAMIEAGAAAVHFEDQLASVKKCGHMGGKVLVPTQEAIQKLVAARL
                     AADVTGVPTLLVARTDADAADLITSDCDPYDSEFITGERTSEGFFRTHAGIEQAISRG
                     LAYAPYADLVWCETSTPDLELARRFAQAIHAKYPGKLLAYNCSPSFNWQKNLDDKTIA
                     SFQQQLSDMGYKFQFITLAGIHSMWFNMFDLANAYAQGEGMKHYVEKVQQPEFAAAKD
                     GYTFVSHQQEVGTGYFDKVTTIIQGGTSSVTALTGSTEESQF"
     gene            3196..4920
                     /gene="aceK"
                     /locus_tag="NBI74_003919"
     CDS             3196..4920
                     /gene="aceK"
                     /locus_tag="NBI74_003919"
                     /EC_number="2.7.11.5"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001137229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional isocitrate dehydrogenase
                     kinase/phosphatase"
                     /protein_id="HCD1934186.1"
                     /translation="MPRGLELLIAQTILQGFDAQYGRFLEVTSGAQQRFEQADWHAVQ
                     QAMKNRIHLYDHHVGLVVEQLRCITNGQSTDAAFLLRVKEHYTRLLPDYPRFEIAESF
                     FNSVYCRLFDHRSLTPERLFIFSSQPERRFRTIPRPLAKDFHPDHGWESLLMRVISDL
                     PLRLRWQNKSRDIHYIVRHLTETLGTDNLAESHLQVANELFYRNKAAWLVGKLITPSG
                     TLPFLLPIHQTDDGELFIDTCLTTTAEASIVFGFARSYFMVYAPLPAALVEWLREILP
                     GKTTAELYMAIGCQKHAKTESYREYLVYLQGCNEQFIEAPGIRGMVMLVFTLPGFDRV
                     FKVIKDKFAPQKEMSAAHVRACYQLVKEHDRVGRMADTQEFENFVLEKRHISPALMAL
                     LLQEAAEKITDLGEQIVIRHLYIERRMVPLNIWLEQVEGQQLRDAIEEYGNAIRQLAA
                     ANIFPGDMLFKNFGVTRHGRVVFYDYDEICYMTEVNFRDIPPPRYPEDELASEPWYSV
                     SPGDVFPEEFRHWLCADPRIGPLFEEMHADLFRADYWRALQNRIREGHVEDVYAYRRR
                     QRFSVRFV"
     gene            complement(4937..5761)
                     /gene="iclR"
                     /locus_tag="NBI74_003920"
     CDS             complement(4937..5761)
                     /gene="iclR"
                     /locus_tag="NBI74_003920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate bypass operon transcriptional
                     repressor IclR"
                     /protein_id="HCD1934187.1"
                     /translation="MVAPIPAKRGRKPAVATAPATGQVQSLTRGLKLLEWIAESNGSV
                     ALTELAQQAGLPNSTTHRLLTTMQQQGFVRQVGELGHWAIGAHAFMVGSSFLQSRNLL
                     AIVHPILRNLMEESGETVNMAVLDQSNHEAIIIDQVQCTHLMRMSAPIGGKLPMHASG
                     AGKAFLAQLSEEQVTKLLHRKGLHAYTHATLVSPVHLKEDLAQTRKRGYSFDDEEHAL
                     GLRCLAACIFDEHREPFAAISISGPISRITDDRVTEFGAMVIKAAKEVTLAYGGIR"
     gene            5962..9645
                     /gene="metH"
                     /locus_tag="NBI74_003921"
     CDS             5962..9645
                     /gene="metH"
                     /locus_tag="NBI74_003921"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HCD1934188.1"
                     /translation="MSSKVEQLCAQLNERILVLDGGMGTMIQSYRLNEADFRGERFAD
                     WPCDLKGNNDLLVLSKPEVISAIHNAYFEAGADIIETNTFNSTTIAMADYQMESLSAE
                     INFAAAKLARACADEWTARTPEKPRYVAGVLGPTNRTASISPDVNDPAFRNITFDQLV
                     AAYRESTKALVEGGADLILIETVFDTLNAKAAVFAVKTEFEALGVELPIMISGTITDA
                     SGRTLSGQTTEAFYNSLRHAEALTFGLNCALGPDELRQYVQELSRIAECYVTAHPNAG
                     LPNAFGEYDLDADTMAKQIREWAEAGFLNIVGGCCGTTPQHIAAMSRAVEGLAPRKLP
                     EIPVACRLSGLEPLNIGEDSLFVNVGERTNVTGSAKFKRLIKEEKYSEALDVARQQVE
                     NGAQIIDINMDEGMLDAEAAMVRFLNLIAGEPDIARVPIMIDSSKWDVIEKGLKCIQG
                     KGIVNSISMKEGVDAFIHHAKLLRRYGAAVVVMAFDEQGQADTRARKIEICRRAYKIL
                     TEEVGFPAEDIIFDPNIFAVATGIEEHNNYAQDFIGACEDIKRELPHALISGGVSNVS
                     FSFRGNDPVREAIHAVFLYYAIRNGMDMGIVNAGQLAIYDDLPTELRDAVEDVILNRR
                     DDGTERLLELAEKYRGSKTDDTANTQQAEWRSWEVNKRLEYSLVKGITEFIEQDTEEA
                     RQQATRPIEVIEGPLMDGMNVVGDLFGEGKMFLPQVVKSARVMKQAVAYLEPFIEASK
                     EQGKTNGKMVIATVKGDVHDIGKNIVGVVLQCNNYEIVDLGVMVPAEKILRTAKEVNA
                     DLIGLSGLITPSLDEMVNVAKEMERQGFTIPLLIGGATTSKAHTAVKIEQNYSGPTVY
                     VQNASRTVGVVAALLSDTQRDDFVARTRKEYETVRIQHGRKKPRTPPVTLEAARDNDF
                     AFDWQAYTPPVAHRLGVQEVEASIETLRNYIDWTPFFMTWSLAGKYPRILEDEVVGVE
                     AQRLFKDANDMLDKLSAEKMLNPRGVVGLFPANRVGDDIEIYRDETRTHVINVSHHLR
                     QQTEKTGFANYCLADFVAPKVSGKADYIGAFAVTGGLEEDALADAFEAQHDDYNKIMV
                     KALADRLAEAFAEYLHERVRKVYWGYAPNENLSNEELIRENYQGIRPAPGYPACPEHT
                     EKATIWELLEVEKHTGMKLTESFAMWPGASVSGWYFSHPDSKYYAVAQIQRDQVEDYA
                     HRKGMSVTEVERWLAPNLGYDAE"
BASE COUNT         2353 a         2504 c         2732 g         2209 t
ORIGIN      
        1 atttttaatt aaaatggaaa ttgtttttga ttttgcattt taaatgagta gtcttagttg
       61 tgctgaacga aaagcgcaca acgatccttc gttcacagtg gggaagtttt cggatccatg
      121 acgaggagct gcacgatgac tgaacaggca acaacaatcg atgaactggc tttcacaagg
      181 ccgtatggcg agcaggagaa gcaaattctt actgccgaag cggtagaatt tctgactgag
      241 ctggtgacgc attttacgcc acaacgcaat aaacttctgg cagcgcgcat tcagcagcag
      301 caggatatcg ataacggaag gttgcctgat ttcatttcgg aaacagcttc cattcgtgat
      361 gctgactgga aaattcgcgg gattcctgcg gacttagaag atcgtcgcgt agagattacc
      421 ggacctgtag agcgcaagat ggtgatcaac gcgctcaacg ccaatgtgaa agtctttatg
      481 gccgatttcg aagattcact ggcaccagac tggaacaaag tgatcgatgg gcaaattaac
      541 ctgcgcgatg cagttaacgg caccatcagc tataccaatg aagcaggcaa aatttatcag
      601 ctcaagccca atccagcggt tttgatttgt cgggtacgcg gtctgcactt gccggaaaaa
      661 catgtcacct ggcgtggtga ggcaatcccc ggtagcctgt ttgattttgc gctctatttc
      721 ttccacaact atcaggcact gttggcaaag ggcagcggtc cctatttcta tctgccgaaa
      781 acccagtcct ggcaggaagc ggcctggtgg agcgaagtct tcagctatgc agaagatcgc
      841 tttaatctgc cgcgcggcac catcaaggcg acgttgctga ttgaaacgct gcccgccgtg
      901 ttccagatgg atgaaatcct tcacgcgctg cgtgaccata ttgttggtct gaactgcggt
      961 cgttgggatt acatcttcag ttatatcaaa acattgaaaa actatcccga tcgcgtcctg
     1021 ccagacagac aggcagtgac gatggataaa cccttcctga atgcttactc acgcctgttg
     1081 attaaaacct gccataaacg cggtgctttt gcgatgggcg gcatggcggc gtttattccg
     1141 agcaaagatg aagagcgcaa taaccaggtg ctcgacaaag taaaagcgga taaatcgctg
     1201 gaagccaata acggtcacga tggcacatgg atcgctcacc cgggccttgc tgatacggca
     1261 atggcggtat tcaacgatat tctcggctcc cgcaaaaacc agcttgaggt gatgcgcgaa
     1321 caagacgcgc cgattactgc cgatcagctg ctggcacctt gtgatggtga acgcaccgaa
     1381 gaaggtatgc gtgccaacat tcgcgtggct gtgcagtaca tcgaagcgtg gatctctggc
     1441 aatggctgtg tgccgattta tggcctgatg gaagatgcgg cgacggctga aatttcccgt
     1501 acctcaatct ggcagtggat ccatcatcaa aaaacgttga gcaatggcaa accggtgact
     1561 aaagccttgt tccgccagat gctgggcgaa gagatgaaag tcattgccag cgaactgggc
     1621 gaagaacgtt tctcccaggg gcgttttgac gatgccgcac gcttgatgga acagatcacc
     1681 acttccgatg agttaattga tttcctgacc ctgccaggct accgcctgtt agcgtaaacc
     1741 accacataac tatggagcat ctgcacatga aaacccgtac acaacaaatt gaagaattac
     1801 agaaagagtg gactcaaccg cgttgggaag gcattactcg cccgtacagc gcggaagatg
     1861 tggtgaaatt acgcggttca gtcaatccgg aatgcaccct ggcgcaactg ggcgcggcga
     1921 aaatgtggcg tctgctgcac ggcgagtcga aaaaaggcta catcaacagc ctcggtgcac
     1981 tgactggcgg tcaggcgttg caacaggcga aagcgggtat tgaagcagtc tatctgtcgg
     2041 gatggcaggt agcggcggac gctaacctgg cggccagcat gtatccggat cagtcgctct
     2101 atccggcaaa ctcggtgcca gctgtggtag agcggatcaa caatactttc cgccgtgcgg
     2161 atcagatcca atggtccgcg ggcattgagc cgggcgatcc gcgctatgtc gattacttcc
     2221 tgccgatcgt cgccgatgcg gaggccggtt ttggcggtgt actgaatgcc tttgaactga
     2281 tgaaagcgat gattgaagcc ggtgcagcgg cagttcactt cgaagatcag ctggcatcag
     2341 tgaagaaatg tggtcacatg ggcggcaaag ttttggtgcc aactcaggaa gctattcaga
     2401 aactggtcgc ggcgcgtctg gcagctgatg tgacgggcgt tccaaccctg ctggttgctc
     2461 gtaccgatgc cgatgcggcg gatctgatta cctccgattg cgacccatat gacagcgaat
     2521 ttattactgg cgagcgtacc agtgaaggct tcttccgtac tcatgcgggt attgagcaag
     2581 cgatcagccg tggcctggcg tatgcgccat atgctgacct ggtatggtgt gaaacctcca
     2641 cgccggatct ggaactggcg cgtcgctttg cacaagccat ccacgcgaaa tatccgggca
     2701 aactgctggc ttataactgc tcgccgtcgt tcaactggca gaaaaacctc gacgacaaaa
     2761 ctattgccag cttccagcag cagctgtcgg atatgggcta caagttccag ttcatcaccc
     2821 tggcaggtat ccacagcatg tggttcaaca tgtttgacct ggcaaacgcc tatgcccagg
     2881 gtgagggtat gaagcactat gttgagaaag tgcagcagcc ggaatttgcc gccgcgaaag
     2941 atggctatac cttcgtatct caccagcagg aagtgggtac aggttatttc gataaagtga
     3001 cgaccattat tcagggcggc acgtcttcag tcaccgcgct gaccggctcc accgaagaat
     3061 cgcagttcta agcaactgat aaagttgatg attgtaggcc ggataaggcg ttcacgccgc
     3121 atccggcaat caacgcctga tgcgacgatt tgcgcgtctt atccggcaat tctctgctcc
     3181 tgatgagggc gctaaatgcc gcgtggcctg gaattactga ttgcacaaac cattctgcaa
     3241 ggcttcgacg ctcagtatgg tcgattcctc gaagtgactt ccggggcgca gcagcgtttc
     3301 gaacaagccg actggcacgc tgtccagcaa gcgatgaaaa accgtatcca tctttacgat
     3361 catcacgtgg gtctggttgt ggagcaactg cgctgcatta ctaacggcca aagcacggac
     3421 gcggcatttt tactgcgcgt taaagagcat tacacccgac tgttgccgga ttacccgcgc
     3481 ttcgagattg cggagagctt ttttaactcc gtgtactgtc ggttatttga ccaccgctcg
     3541 cttactcccg agcggctttt tatctttagc tcccagccag agcgccgttt tcgtaccatt
     3601 ccccgcccgc tggcgaaaga ctttcacccc gatcacggct gggaatctct gctgatgcgc
     3661 gttatcagcg acctgccgtt gcgtctgcgc tggcagaata aaagccgtga cattcattac
     3721 atcgttcgcc atctaacgga aacgctgggg acagacaacc tcgcggaaag tcatttacag
     3781 gtggcgaacg aactgtttta ccgcaataaa gccgcctggc tggtgggcaa actgatcacg
     3841 ccttccggca cattgccatt tttgctgccg atccaccaga cggacgacgg cgagttattt
     3901 attgatacct gtctgacgac gaccgccgaa gcgagcatcg tctttggctt tgcgcgttct
     3961 tattttatgg tttacgcgcc gctgcccgca gcgctggtcg agtggctacg ggaaattctg
     4021 ccaggtaaaa ccaccgctga attgtatatg gctatcggct gccagaagca cgccaaaacc
     4081 gaaagctacc gcgaatatct cgtttatcta cagggctgta atgagcagtt cattgaagcg
     4141 ccgggtattc gtggaatggt gatgttggtg ttcacgctgc cgggctttga ccgggtattt
     4201 aaagtgatca aagacaagtt cgcgccgcag aaagagatgt ctgccgctca cgttcgcgcc
     4261 tgctaccaac tggtgaaaga gcacgatcgc gtgggccgaa tggcggacac ccaggagttt
     4321 gaaaactttg tgctggagaa gcggcatatt tccccggcat taatggcatt actgctccag
     4381 gaagcagcgg aaaaaatcac cgatctcggc gaacaaattg tgattcgcca tctttatatt
     4441 gagcggcgga tggtgccgct caatatctgg ctggaacaag tggaaggtca gcagttgcgc
     4501 gatgccattg aagaatacgg taacgctatt cgccagcttg ccgctgctaa cattttccct
     4561 ggcgacatgc tgtttaaaaa cttcggtgtc acccgtcacg ggcgtgtggt gttttatgat
     4621 tacgatgaaa tttgctacat gacggaagtg aacttccgcg acatcccgcc gccgcgctac
     4681 ccggaggacg aacttgccag cgaaccgtgg tacagcgtct cgccgggcga tgttttcccg
     4741 gaagagttcc gtcactggtt atgtgccgac ccgcgtattg gtccgctatt tgaagagatg
     4801 cacgccgact tgttccgcgc tgattactgg cgcgcactac aaaaccgtat acgtgaaggg
     4861 catgtggaag atgtttatgc gtatcggcgc aggcagcggt ttagcgttcg attcgtataa
     4921 atcagaggcg aaaaagtcag cgtattccac cgtatgccag cgtcacttcc ttcgccgctt
     4981 taatcaccat cgcgccaaac tcggtcacgc ggtcatcggt aatacgtgaa atcggtccgg
     5041 aaattgaaat tgcggcaaac ggttcgcggt gctcatcgaa aatacacgct gcaaggcaac
     5101 gcagccccag cgcatgttcc tcatcgtcaa atgaataacc ccgtttgcgc gtttgggcga
     5161 gatcttcttt taaatgcaca ggagacacca gcgttgcgtg ggtataggca tgtaaccctt
     5221 tgcggtgcag taacttcgtc acctgttctt cgctcagttg ggccagaaag gctttacccg
     5281 caccggaagc gtgcatcggc aatttaccgc cgataggcgc ggacattcgc atcaggtgag
     5341 tacactgtac ctggtcgata ataatcgctt cgtgattgct ttgatcaagc accgccatat
     5401 tgaccgtttc gccagactct tccattagat tgcgcaggat agggtgaaca atcgctaaca
     5461 aattacggct ctggagaaag ctgctgccga ccataaaggc gtgtgcgccg atcgcccagt
     5521 gacccagttc gccgacctga cggacaaagc cctgttgctg catcgtggtt agcaggcggt
     5581 gggtcgtgga attgggtaac ccggcttgct gtgccagttc cgtgagtgcc acactgccat
     5641 tggattcggc aatccactcc agtaatttca ggccacgcgt taaagactga acctgtccag
     5701 tcgctggtgc ggtggcaacg gcgggttttc tgccgcgttt tgcgggaatg ggtgcgacca
     5761 tgacagtctc cttttttctg tatcgtggaa atcattttca tttttattat cagcgaatgc
     5821 aatagttgct gaacagatcc gatgagttaa tgttgaacaa atctcatgtt gcgtggtggt
     5881 cgcttttacc acagatgtgt ttatgccagt atggtttgtt gaatttttat taaatctggg
     5941 ttaagcgtgt cgggagcaag tgtgagcagc aaagtggaac aactgtgtgc gcagttaaat
     6001 gaacgtattc tggtgctgga cggcggtatg ggcaccatga tccagagcta tcgactgaac
     6061 gaagccgatt ttcgtggtga acgctttgcc gactggccat gcgatctcaa aggcaacaac
     6121 gacctgctgg tgctcagcaa accggaagtg atctccgcca tccacaacgc ttactttgaa
     6181 gcgggtgcgg atatcatcga aaccaacacc ttcaactcca cgactattgc gatggcggat
     6241 taccagatgg aatccctgtc ggcggaaatc aactttgcgg cggcgaaact ggcgcgcgcc
     6301 tgtgccgacg agtggactgc ccgcacgccg gagaaaccgc gctacgttgc cggtgttctc
     6361 ggcccgacca accgcacggc gtctatttct ccggacgtca acgatccggc attccgcaac
     6421 atcacttttg accagctggt ggcggcttat cgagaatcca ccaaagcgct ggtggaaggt
     6481 ggcgcggacc tgatcctgat tgaaaccgtt ttcgacaccc ttaacgccaa agcggcggtc
     6541 tttgcggtga aaacggagtt tgaagcgctg ggcgttgaac tgccgattat gatctccggc
     6601 accatcaccg acgcctccgg gcgcacgctc tccgggcaga ccactgaagc gttttacaac
     6661 tccctgcgcc acgccgaagc gctgaccttt ggcctgaact gtgcgctggg gccagacgaa
     6721 ctgcgccagt acgtacagga actgtcgcgt attgcggaat gctatgtcac cgcccacccg
     6781 aacgccgggc tgcccaacgc ctttggtgaa tacgatctcg acgccgacac gatggcaaaa
     6841 cagatacgtg aatgggcaga agcgggcttt ctcaatatcg tcggcggctg ctgcggcacc
     6901 acgccacaac atattgcagc gatgagtcgt gcagtagaag gattagcgcc gcgcaaacta
     6961 ccggaaattc ccgtagcctg ccgtttgtcc ggcctggagc cgctgaacat tggcgaagat
     7021 agcctgtttg tgaacgtggg tgaacgcacc aacgtcaccg gttccgctaa gttcaagcgc
     7081 ctgatcaaag aagagaaata cagcgaggcg ctggatgtgg cgcgtcagca ggtggaaaac
     7141 ggtgcgcaga ttatcgatat caacatggat gaagggatgc tcgatgccga agcggcgatg
     7201 gtgcgttttc tcaatctgat tgccggtgaa ccggatatcg ctcgtgtgcc gataatgatc
     7261 gactcctcga aatgggacgt tattgaaaaa ggtctgaagt gtatccaggg caaaggcatt
     7321 gttaactcta tctcgatgaa agagggcgtc gacgccttta tccatcacgc gaaactgttg
     7381 cgtcgctacg gtgcggcagt agtggtaatg gcttttgacg aacagggcca ggccgatacc
     7441 cgcgcacgga aaatcgagat ttgccgtcgg gcgtacaaaa tcctcaccga agaggttggc
     7501 ttcccggctg aagatatcat cttcgacccg aacatcttcg cggtcgcaac tggcattgaa
     7561 gagcacaaca attacgcaca ggactttatc ggcgcgtgtg aagacatcaa acgtgaactg
     7621 ccgcacgcac tgatttccgg cggcgtttct aacgtgtcgt tctcgtttcg tggtaacgat
     7681 cctgtgcgcg aagccattca tgcggtgttc ctctactacg ctattcgcaa tggcatggat
     7741 atggggatcg tcaacgccgg gcaactggcg atttacgacg acctgcctac cgagctacgc
     7801 gacgcggtgg aggatgtgat cctcaaccgc cgcgacgatg gcaccgagcg tttactcgag
     7861 cttgccgaga aatatcgcgg cagcaaaacc gacgacaccg ctaacaccca gcaggcggag
     7921 tggcgctcgt gggaagtgaa taaacgtctg gaatactcgc tggtcaaagg cattaccgag
     7981 tttatcgagc aggataccga agaagcccgc cagcaggcta cgcgcccgat tgaagtgatt
     8041 gaaggcccgt tgatggacgg catgaacgtg gtcggcgacc tgtttggcga gggcaaaatg
     8101 ttcctgccgc aggtggtgaa atcggcgcgc gtaatgaagc aggcggtggc gtatctcgag
     8161 ccgtttattg aagccagcaa agagcagggt aaaaccaacg gcaagatggt gatcgccaca
     8221 gtgaagggcg atgtccacga catcggtaaa aatatcgttg gcgtggtgct gcaatgtaac
     8281 aactacgaaa ttgtcgatct cggcgtgatg gtgcctgcgg aaaaaattct ccgtaccgct
     8341 aaagaagtga atgctgattt gattggcctt tcggggctga tcacgccgtc gctggacgag
     8401 atggttaacg tggcgaaaga gatggagcgc cagggcttca ccattccgct attgattggc
     8461 ggcgcgacca cctcaaaagc gcacacggcg gtgaaaatcg agcagaacta cagcggcccg
     8521 acggtgtatg tgcagaacgc atcgcgcacc gttggtgtgg tggcggcgct gctttccgac
     8581 actcagcgcg atgatttcgt tgctcgtacc cgcaaggagt acgaaaccgt gcgtattcag
     8641 cacggacgaa agaaaccacg cacaccaccg gtcacgctgg aagcggcgcg tgataacgac
     8701 ttcgcttttg actggcaggc ttacacgccg ccggtggcgc accgtctcgg tgtgcaggaa
     8761 gtcgaagcca gcatcgaaac gctgcgtaat tacatcgact ggacgccgtt ctttatgacc
     8821 tggtcgctgg ccgggaagta tccgcgcatt ctggaagatg aagtggtagg tgttgaggcg
     8881 cagcggctgt ttaaagacgc caacgacatg ctggataaat taagcgccga aaaaatgctg
     8941 aacccgcgtg gtgtggtggg cctgttcccg gcaaaccgtg tgggcgatga cattgaaatc
     9001 taccgtgacg aaacgcgtac ccatgtgatt aatgtcagcc accatctgcg ccaacagacc
     9061 gaaaaaaccg gcttcgctaa ctactgtctc gctgacttcg ttgcgccgaa ggtttccggc
     9121 aaagcggatt acatcggcgc atttgccgtg actggcgggc tggaagagga cgcactggct
     9181 gatgcctttg aagcacagca tgatgattac aacaaaatca tggtgaaagc gcttgccgac
     9241 cgtctggcgg aagcctttgc ggagtatctc catgagcgtg tgcgtaaagt ctactggggc
     9301 tatgcgccga acgagaacct cagcaacgaa gagctgatcc gcgaaaacta ccagggcatc
     9361 cgtccggcac cgggctaccc tgcctgcccg gaacatacgg aaaaagccac aatctgggag
     9421 ctgctggaag tggaaaaaca taccggtatg aaactcacgg aatctttcgc catgtggccg
     9481 ggtgcatcgg tttcgggttg gtacttcagt catccagaca gcaagtatta cgcggtggcg
     9541 caaattcagc gcgatcaggt tgaagactat gcccaccgta aaggaatgag cgttaccgaa
     9601 gttgagcgtt ggttggcacc gaatctgggg tatgatgccg agtaagtgat aacaccgggg
     9661 tatttgcccc ggtttttaat gtcataaata atggattatt gaatatgata aaaattgatg
     9721 aaatagattt tttcgctttc gaaggatgtt cagagtccga agttactgaa ctattaggaa
     9781 aaaaccaaca ggttataa
//
LOCUS       DAHHTG010000152         9720 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.155, whole genome shotgun sequence.
ACCESSION   DAHHTG010000152 DAHHTG010000000
VERSION     DAHHTG010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9720)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9720)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9720
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.155"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            136..1113
                     /gene="csiD"
                     /locus_tag="NBI74_003922"
     CDS             136..1113
                     /gene="csiD"
                     /locus_tag="NBI74_003922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311547.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation induced protein CsiD"
                     /protein_id="HCD1934189.1"
                     /translation="MNALTAVQNNAVDSGQDYRGFTLIPSAQSPRLLELTFTEQTTKQ
                     FLEQVAEWPVQALEYKSFLRFQVGKILDDLCANQLQPLLLKTLLNRAEGALLINAVGI
                     DDVAQADEMVKLATAVAHLIGRSNFDAMSGQYYARFVVKNVDNSDSYLRQPHRVMELH
                     NDGTYVEEITDYVLMMKIDEQNIQGGNSLLLHLDDWEHLDLFFRHPLARRPMRFAAPP
                     SKNVSKDVFHPVFDVDQQGRPVMRYIDQFVQPKDFEEGVWLSELSDAIETSKGTLSVP
                     VPVGKFLLINNLFWLHGRDRFTPHPDLRRELMRQRGYFAYATHHYQTHQ"
     gene            1133..2398
                     /gene="lhgO"
                     /locus_tag="NBI74_003923"
     CDS             1133..2398
                     /gene="lhgO"
                     /locus_tag="NBI74_003923"
                     /EC_number="1.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-hydroxyglutarate oxidase"
                     /protein_id="HCD1934190.1"
                     /translation="MYDFVIIGGGIIGMSTAMQLIDIYPDARIALLEKESGPACHQTG
                     HNSGVIHAGVYYTPGSLKTQFCLAGNRATKVFCDQNGIRYDNCGKMLVATSELEMERM
                     NALWKRTAANGIEREWLNAEELREREPNITGLGGIFVPSSGIVSYREVTAAMAKIFQA
                     RGGEIIYNAEVSALSEHKNGVVIRTRQGGEYEASTLISCSGLMADRLVKMLGLEPGFI
                     ICPFRGEYFRLAPEHNQIVNHLIYPIPDPAMPFLGVHLTRMIDGSVTVGPNAVLAFKR
                     EGYRKRDFSFSDTLEILGSSGIRRVLQNHLRSGLGEMKNSLCKSGYLRLVQKYCPRLS
                     LSDLQPWPPGVRAQAVSPDGKLIDDFLFVTTPRTIHTCNAPSPAATSAIPIGAHIVSK
                     VQTLLASQSNPGRTLRAARSVDALHAAFN"
     gene            2424..3872
                     /gene="gabD"
                     /locus_tag="NBI74_003924"
     CDS             2424..3872
                     /gene="gabD"
                     /locus_tag="NBI74_003924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HCD1934191.1"
                     /translation="MKLNDSNLFRQQALINGEWLDANNGEVIDVTNPANGDKLGSVPK
                     MGADETRAAIDAASRALPAWRALTAKERANILRNWFNLMMEHQDDLARLMTLEQGKPL
                     AEAKGEISYAASFIEWFAEEGKRIYGDTIPGHQADKRLIVIKQPIGVTAAITPWNFPA
                     AMITRKAGPALAAGCTMVLKPASQTPFSALALAELAIRAGVPAGVFNVVTGSAGAVGN
                     ELTSNPLVRKLSFTGSTEIGRQLMEQCAKDIKKVSLELGGNAPFIVFDDADLDKAVEG
                     ALASKFRNAGQTCVCANRLYVQDSVYDRFAEKLQQAVSKLHIGDGLEKGVTIGPLIDE
                     KAVAKVEEHIADALEKGARVVCGGKAHERGGNFFQPTILVDVPANAKVSKEETFGPLA
                     PLFRFKDEADVIAQANDTEFGLAAYFYARDLSRVFRVGEALEYGIIGINTGIISNEVA
                     PFGGIKASGLGREGSKYGIEDYLEIKYMCIGL"
     gene            3886..5166
                     /gene="gabT"
                     /locus_tag="NBI74_003925"
     CDS             3886..5166
                     /gene="gabT"
                     /locus_tag="NBI74_003925"
                     /EC_number="2.6.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000097653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="HCD1934192.1"
                     /translation="MISNKELMQRRSQAIPRGVGQIHPIFADRAENCRVWDVEGREYL
                     DFAGGIAVLNTGHLHPKVVAAVEAQLKKLSHTCFQVLAYEPYLELCEIMNQKVPGDFA
                     KKTLLVTTGSEAVENAVKIARAATKRSGTIAFSGAYHGRTHYTLALTGKVNPYSAGMG
                     LMPGHVYRALYPCPLHGISEDDAIASIHRIFKNDAAPEDIAAIVIEPVQGEGGFYAAT
                     PAFMQRLRALCDEHGIMLIADEVQSGAGRTGTLFAMEQMGVAPDLTTFAKSIAGGFPL
                     AGVTGRAEVMDAVAPGGLGGTYAGNPIACVAALEVLKVFEQENLLQKANVLGQKLKDG
                     LLAIAEKHPEIGDVRGLGAMIAIELFEDGDHSKPDAKLTAEIVARARDKGLILLSCGP
                     YYNVLRILVPLTIEDAQIRQGLEIISQCFAEAKL"
     gene            5403..6803
                     /gene="gabP"
                     /locus_tag="NBI74_003926"
     CDS             5403..6803
                     /gene="gabP"
                     /locus_tag="NBI74_003926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GABA permease"
                     /protein_id="HCD1934193.1"
                     /translation="MGQSSQPHELGGGLKSRHVTMLSIAGVIGASLFVGSSVAIAEAG
                     PAVLLAYLFAGLLVVMIMRMLAEMAVATPDTGSFSTYADKAIGRWAGYTIGWLYWWFW
                     VLVIPLEANIAAMILHSWVPGIPIWLFSLVITLALTGSNLLSVKNYGEFEFWLALCKV
                     IAILAFIFLGAVAISGFYPYADVSGISRLWDSGGFMPNGFGAVLSAMLITMFSFMGAE
                     IVTIAAAESDTPEKHIVRATNSVIWRISIFYLCSIFVVVALIPWNMPGLKAVGSYRSV
                     LELLNIPHAKLIMDCVILLSVTSCLNSALYTASRMLYSLSRRGDAPAVMGKINRSKTP
                     YVAVLLSTSAAFLTVVVNYYAPAKVFKFLIDSSGAIALLVYLVIAVSQLRMRKILRAE
                     GSEIRLRMWLYPWLTWLVIGFITFVLVVMLFRPAQQLEVISTGLLAIGIICTVPIMAR
                     WKKLIMWQKTPIHNTR"
     gene            6824..7486
                     /gene="csiR"
                     /locus_tag="NBI74_003927"
     CDS             6824..7486
                     /gene="csiR"
                     /locus_tag="NBI74_003927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311552.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CsiR"
                     /protein_id="HCD1934194.1"
                     /translation="MTITSLDGYRWLKNDIIRGNFQPDEKLRMSLLTSRYALGVGPLR
                     EALSQLVAERLVTVVNQKGYRVASMSEQELLDIFDARANMEAMLVSLAIARGGDEWEA
                     DVLAKAHLLSKLEACDASEKMLDEWDLRHQAFHTAIVAGCGSYYLLQMRERLFDLAAR
                     YRFIWLRRTVLSVEMLEDKHDQHQTLTAAVLARDTARASELMRQHLLTPIPIIQQAMA
                     GN"
     gene            complement(7487..7936)
                     /gene="kbp"
                     /locus_tag="NBI74_003928"
     CDS             complement(7487..7936)
                     /gene="kbp"
                     /locus_tag="NBI74_003928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium binding protein Kbp"
                     /protein_id="HCD1934195.1"
                     /translation="MGLFNFVKDAGEKLWDAVTGQHDKDDQAKKVQEHLNKTGIPDAD
                     KVNIQIADGKATVTGDGLSQEAKEKILVAVGNISGIASVDDQVKTATPATASQFYTVK
                     SGDTLSAISKQVYGNANLYNKIFEANKPMLKSPDKIYPGQVLRIPEE"
     gene            complement(8020..8178)
                     /locus_tag="NBI74_003929"
     CDS             complement(8020..8178)
                     /locus_tag="NBI74_003929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqaE/Pmp3 family membrane protein"
                     /protein_id="HCD1934196.1"
                     /translation="MGFWRIVITIILPPLGVLLGKGFGWAFIINILLTLLGYIPGLIH
                     AFWVQTRD"
     gene            8361..8660
                     /locus_tag="NBI74_003930"
     CDS             8361..8660
                     /locus_tag="NBI74_003930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCD1934197.1"
                     /translation="MTELAQLQASAEQAAALLKSMSHPKRLLILCMLSGSPGTSAGEL
                     TRITGLSASATSQHLARMRGEGLIDCQRDAQRILYSIKNEAVNAIIATLKNVYCP"
     gene            8670..9194
                     /gene="ygaP"
                     /locus_tag="NBI74_003931"
     CDS             8670..9194
                     /gene="ygaP"
                     /locus_tag="NBI74_003931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YgaP"
                     /protein_id="HCD1934198.1"
                     /translation="MALTTISPHDAQELIARGAKLIDIRDADEYLREHIPEADLAPLS
                     VLEQSGLPPKLRREQIIFHCQAGKRTSNNADKLAAIAAPAEIFLLEDGIDGWKKAGLP
                     VAVNKSQPLPLMRQVQIAAGGLILIGVVLGYTVNSVFFLLSGFVGAGLLLAGISGFCG
                     MARLLDKMPWNQRA"
     gene            complement(9241..9645)
                     /gene="stpA"
                     /locus_tag="NBI74_003932"
     CDS             complement(9241..9645)
                     /gene="stpA"
                     /locus_tag="NBI74_003932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein StpA"
                     /protein_id="HCD1934199.1"
                     /translation="MSVMLQSLNNIRTLRAMAREFSIDVLEEMLEKFRVVTKERREEE
                     EQQQRELAERQEKISTWLELMKADGINPEELLGNSSAAAPRAGKKRQPRPAKYKFIDV
                     NGETKTWTGQGRTPKPIAQALAEGKSLDDFLI"
BASE COUNT         2229 a         2512 c         2659 g         2320 t
ORIGIN      
        1 tgctgttttt gatcacaata agaaaacaat atgtaacttt tatgcgcatt tttcagaaat
       61 gtagatattt ttagattatg gctacgaaat gagcatcgcc atgtcaccct acatctcata
      121 agaggatcgc ttctgatgaa tgcactgacc gccgtacaaa ataacgctgt cgattcaggc
      181 caggactatc gcggattcac tctcatcccg tcggcgcaat ccccgcgtct gctggaactc
      241 accttcaccg aacagacgac caaacagttt ctcgaacagg ttgccgaatg gcccgtgcag
      301 gcgctggagt acaaatcgtt tctgcgtttt caggtaggca aaattctcga cgatctgtgt
      361 gcgaatcagc tgcaaccgct gctgttgaag accctgttaa accgcgctga aggtgcgctg
      421 ttgatcaatg cggtgggtat cgatgatgtc gcgcaggcgg atgagatggt gaagctggcg
      481 acggcggtgg cgcatctgat tggccgttcc aatttcgatg ccatgagcgg tcagtattac
      541 gcgcgtttcg tggtgaaaaa tgttgataac tcagacagct atcttcgtca gccgcaccgc
      601 gtaatggagc tgcacaacga cggcacttac gtcgaagaga tcactgatta cgtgctgatg
      661 atgaaaatcg acgagcaaaa catacagggc ggtaactcgc tgctgctgca tctcgatgac
      721 tgggaacatc tggacctctt tttccgccat ccgctggcgc gccgtccgat gcgctttgcc
      781 gcgccgccga gcaaaaacgt cagcaaagat gtcttccatc cggtgttcga tgtcgatcag
      841 cagggccgcc cagtgatgcg ctatatcgac cagtttgtcc agccgaaaga cttcgaagaa
      901 ggcgtgtggt tgagcgagct ttcggacgcc attgaaacca gtaaaggcac tctttctgta
      961 cccgttcccg ttggcaaatt cctgttgatt aacaacctgt tctggctgca cggtcgcgac
     1021 cgctttactc cgcacccgga tctgcgccgt gaactgatgc gtcagcgtgg ctatttcgct
     1081 tacgccactc accactacca gacgcatcag taagcgcaaa ggaattgagc ggatgtatga
     1141 ttttgtgatt attggcggcg gcatcatcgg catgtcgacc gccatgcaac tgattgatat
     1201 ctatcctgac gcccgcattg cgttgctgga aaaagagtcc ggccctgcct gtcaccagac
     1261 gggccacaac agcggcgtga tccatgccgg agtctattac acgcccggta gcctgaagac
     1321 gcagttttgc ctggcgggga accgcgccac taaagtcttt tgcgatcaaa acggcattcg
     1381 ctacgacaac tgcggcaaga tgctggtcgc cacgtctgaa ctcgaaatgg aacggatgaa
     1441 tgcgttatgg aaacgcacgg cggcaaacgg gatcgagcgc gagtggttaa acgccgagga
     1501 gctgcgcgag cgtgaaccga atatcaccgg gcttggcggc atttttgtgc cgtccagcgg
     1561 cattgtcagc taccgcgaag tgacggcggc gatggcaaaa atcttccagg ccagaggtgg
     1621 tgagattatt tataacgccg aagtcagcgc cctcagtgag cataaaaacg gcgtggtgat
     1681 acgtacccgt cagggcggtg aatatgaagc atcgacgctg attagttgtt ccgggctgat
     1741 ggctgaccgg ctggtgaaaa tgctcggcct cgaaccgggc tttattatct gcccgttccg
     1801 cggcgagtat ttccgccttg cgccagagca taaccagatt gttaaccacc tgatttaccc
     1861 cattcccgac ccggcaatgc cgtttttggg cgtgcatctc acccgcatga tcgacggcag
     1921 cgtgaccgtc gggccaaacg cggtgctggc tttcaaacgc gaaggctacc gcaagcgcga
     1981 cttctcattt agtgacacgc tggagatttt aggctcgtcg gggattcgcc gggtgctgca
     2041 aaaccatcta cgctcaggac tgggcgagat gaaaaactcg ctgtgcaaaa gcggctatct
     2101 gcggctggtg caaaagtatt gtccccggct ttcgttaagc gatctccagc cctggccccc
     2161 cggtgtgcgg gcgcaggcgg tatcgccgga cggcaagctg attgacgatt ttctgtttgt
     2221 caccaccccg cgcacgatcc acacctgtaa tgcgccctcc ccggcagcga catcagcaat
     2281 tcctattggt gcgcatattg tcagcaaggt acaaacgctg ttggcaagcc agagtaaccc
     2341 cggacgcacg ctgcgagcgg cacgtagtgt ggatgcctta cacgccgcat ttaattaata
     2401 acctttgaaa acaggatgta gcgatgaaac ttaacgacag taacttattc cgccagcagg
     2461 cgttgattaa cggggaatgg ctggacgcca acaatggcga ggtcatcgac gtcaccaatc
     2521 cggcgaacgg cgacaagctg ggtagcgtgc cgaaaatggg cgctgatgaa acccgcgccg
     2581 ctatcgacgc cgccagccgc gccctgcccg cctggcgtgc gctcactgcc aaagaacgcg
     2641 ccaacattct gcgcaactgg ttcaatttaa tgatggagca tcaggacgat ttagcgcgcc
     2701 tgatgaccct cgaacagggc aaaccactgg ctgaagcgaa aggcgaaatc agctacgccg
     2761 cctcatttat tgagtggttt gctgaagaag gcaaacgtat ttatggcgac accattcccg
     2821 gtcatcaggc cgataaacgc ctgattgtga tcaagcagcc gattggcgtc accgcggcta
     2881 tcacgccatg gaacttcccg gcggcgatga ttacccgtaa agctggtccg gcgctggcgg
     2941 ctggctgcac catggtgctg aagcccgcca gtcagacgcc gttctctgcg ctggcgctgg
     3001 cagaactggc gattcgcgcg ggcgttccgg ctggcgtgtt taacgtggtc accggttcgg
     3061 cgggcgcggt cggtaacgaa ctgaccagca acccgctggt gcgcaagctg tcgtttaccg
     3121 gctcgaccga aattggccgt cagttaatgg aacagtgcgc gaaagacatc aaaaaagtgt
     3181 cgctggagct gggcggcaac gcaccgttta ttgtctttga cgatgccgat ctcgataaag
     3241 ccgtggaagg cgcgctggcc tcgaaattcc gtaacgccgg gcaaacctgc gtctgcgcca
     3301 accgcctgta tgtgcaggac agcgtgtatg accgctttgc cgaaaaattg cagcaggcgg
     3361 tgagcaaact gcacatcggc gacgggctgg agaaaggcgt caccatcggg ccgttgatcg
     3421 atgaaaaagc ggtagcaaaa gtggaagagc atattgctga tgcgctggag aaaggcgcgc
     3481 gcgtggtttg cggcggtaaa gcacatgaac gcggcggtaa cttcttccag ccgaccattc
     3541 tggtagacgt tccggctaac gccaaagtgt cgaaagaaga gacgtttggc cccctcgccc
     3601 cgctgttccg ttttaaagat gaagccgatg tgattgcgca agccaatgac accgaatttg
     3661 gacttgcagc ctatttctac gcccgtgatt taagccgcgt cttccgcgtg ggcgaagcac
     3721 tggagtacgg catcatcggc atcaacaccg gcattatttc caatgaagtg gccccgttcg
     3781 gcggcattaa agcctcgggt ctgggtcgtg aaggttcgaa gtatggcatc gaagattact
     3841 tagaaatcaa atatatgtgc atcggtcttt aactggagaa tgcgaatgat cagcaataaa
     3901 gagttaatgc agcgccgcag tcaggcgatt ccccgtggcg ttgggcaaat tcacccgatt
     3961 ttcgctgacc gcgcggaaaa ctgccgggtg tgggacgttg aaggccgtga gtatcttgat
     4021 ttcgcgggcg ggattgcggt gctcaatacc gggcacctgc atccgaaagt ggttgcggcg
     4081 gtggaagcgc aattgaaaaa actgtcgcac acctgcttcc aggtgctggc ctacgagccg
     4141 tatctggagc tgtgcgagat tatgaatcag aaggtgccgg gcgattttgc caagaaaact
     4201 ctgctggtta cgaccggttc cgaagcggtg gaaaatgcgg tgaaaatcgc ccgcgccgcc
     4261 actaaacgta gcggtactat cgcctttagc ggtgcgtatc acgggcgcac gcattacacg
     4321 ctggcgctga ccggcaaggt gaatccgtac tctgcgggca tggggctgat gccgggtcat
     4381 gtttatcgcg cgctttatcc ttgcccgctg cacggcataa gcgaggatga cgctatcgcc
     4441 agcatccacc ggatcttcaa aaatgacgcc gcgccggaag atatcgccgc catcgtgatt
     4501 gagcccgttc agggcgaagg cggtttctac gccgcgacgc cagcctttat gcagcgttta
     4561 cgcgcgctgt gtgacgagca cgggatcatg ctgattgccg atgaagtgca gagcggcgca
     4621 gggcgtaccg gcacgctgtt tgcgatggag caaatgggcg tggcaccaga tctcaccacc
     4681 tttgcgaaat cgatcgcagg cggtttcccg ctggcgggcg tcaccgggcg cgcggaagtg
     4741 atggacgccg tcgcaccggg cggactgggt ggcacctatg ccggtaaccc gattgcctgc
     4801 gtagcggcgc tggaagtgtt gaaggtgttc gagcaggaaa atctgctgca aaaagccaac
     4861 gttctggggc agaagctgaa agacggattg ctggcgatag ccgaaaaaca cccggagatc
     4921 ggcgacgtac gcggtctggg ggcgatgatc gccatcgagc tgtttgaaga tggcgatcac
     4981 agcaaaccgg acgcaaaact caccgccgag atcgtggcac gcgcccgcga taagggtctg
     5041 attcttctct cctgcggccc gtattacaac gtgctgcgca tccttgtacc gctcaccatt
     5101 gaagacgctc agatccgtca gggtctggag atcatcagcc agtgttttgc tgaggcgaag
     5161 ctgtagcacc gcaccatgcc ggaggcgacg ctttgcggct tgtccggcct acgagaatcc
     5221 aggtcggata aggcgtttac gccgcatccg acaatctgta cgtgaacagg aataaatcta
     5281 tgtcggccgg ataaggcgga gccgctctcc ggcaaaaaga accaataaca attaaacgcg
     5341 tgacccgacg cgggaaatgt cggggcgctc tcccaagtga cacactttcg agaggattca
     5401 ggatggggca atcatcgcaa ccacatgagt taggcggcgg gctgaagtca cgccacgtca
     5461 ccatgttgtc tattgccggt gttatcggcg caagtctgtt tgtcggttcc agcgtcgcca
     5521 tcgccgaagc gggcccggcg gtattactgg cctatctgtt cgccggatta ctggtggtta
     5581 tgattatgcg gatgttggcg gaaatggcgg ttgccacgcc cgataccggt tcgttttcca
     5641 cctatgccga taaagccatt ggccgctggg cgggctatac catcggctgg ttgtactggt
     5701 ggttttgggt actggttatc ccacttgaag ccaacatcgc cgccatgatc ctgcactcat
     5761 gggttccagg cattcccatc tggttatttt ccctcgtcat taccctcgcc ttaaccggca
     5821 gtaatttatt aagcgttaaa aactacggcg aatttgagtt ctggctggcg ctgtgtaaag
     5881 tcatcgctat cctggccttt attttccttg gtgccgtcgc aattagcggt ttttacccgt
     5941 atgccgacgt gagcgggatt tcaagattgt gggatagcgg cggctttatg cccaacggct
     6001 tcggtgcggt attaagcgcg atgttgatca ccatgttctc gtttatgggc gcagaaattg
     6061 tcaccattgc cgccgcggaa tccgacacgc cggaaaaaca tattgttcgc gccaccaact
     6121 cggttatctg gcgtatctct attttctatt tgtgttctat ttttgtcgtt gttgcattaa
     6181 tcccgtggaa tatgccaggg ctgaaagccg ttggttctta tcgctcggtt ctggaattgc
     6241 tcaatattcc tcatgcgaaa ttaatcatgg actgcgtgat attactttcc gtaaccagtt
     6301 gcctgaactc ggcgctgtat accgcttcca ggatgctcta ctccttaagc cgtcgcggtg
     6361 atgctcccgc ggtaatgggt aaaatcaacc gcagtaaaac cccgtacgta gcggtgttac
     6421 tctccaccag cgcggcattc ttaacggtgg tggtgaacta ttacgcgcct gcgaaggtgt
     6481 ttaaatttct gatcgacagc tccggcgcta tcgccctgct ggtttattta gtcatcgccg
     6541 tttcacaatt gcggatgcgc aaaattctgc gagcagaagg aagcgaaatt cgcctgcgga
     6601 tgtggcttta tccgtggctc acctggctgg tcatcggctt tattaccttt gtgttggtag
     6661 tgatgctatt ccgcccggcg caacagttag aagtgatctc caccggctta ttagcgattg
     6721 ggattatctg taccgtgcca attatggctc gctggaaaaa gctgataatg tggcaaaaaa
     6781 cacccattca taatacgcgc tgataatgat caggagtcac accatgacca ttacctctct
     6841 ggatggctat cgctggctga agaacgatat tattcgcggc aattttcagc cggatgaaaa
     6901 attacggatg agtttgctga catcgcgtta tgcacttggc gttgggccgt tgcgggaagc
     6961 actttcgcaa ctggtggcag aacgactggt cacggtggtg aatcaaaaag ggtatcgggt
     7021 ggcgtctatg tcagagcagg agctgctcga tattttcgac gcccgcgcca atatggaagc
     7081 gatgctggtg agtctggcga ttgcccgcgg tggcgatgaa tgggaggcag acgttctcgc
     7141 aaaagcgcat ctgctcagta agcttgaggc ctgtgacgcc agtgagaaaa tgcttgatga
     7201 gtgggatctg cgtcatcagg cgtttcatac tgcaattgtg gcgggctgtg gttcttacta
     7261 tttgctgcaa atgcgtgaac ggttgtttga tctggcagcg cgttatcgat ttatctggct
     7321 gcggcgaaca gtgctttcgg tggaaatgct ggaagataaa cacgatcagc accagaccct
     7381 gactgcggcg gtactggcgc gagataccgc gcgcgccagt gagttaatgc gccagcattt
     7441 actgacgcca attcccatta tccagcaggc gatggctggc aattaactac tcttcaggaa
     7501 tacgcaacac ttgccccggg taaattttat ccgggctttt tagcatcggt ttattcgctt
     7561 cgaagatttt attgtacaga ttagcgttac cgtagacctg tttggaaatg gcactcagag
     7621 tgtcgccaga cttaacggta taaaactggc tggcagtggc tggtgtcgcc gttttcacct
     7681 gatcatcgac actggcaata ccggaaatat tccccaccgc aacaaggatt ttctccttcg
     7741 cctcctgact taagccgtca ccagtgaccg tcgctttgcc gtcggcaatt tgaatgttca
     7801 ctttatcggc atcaggtata ccggttttgt tcagatgctc ctgcaccttc ttcgcctgat
     7861 cgtctttatc gtgctgacct gtaaccgcgt cccagagttt ttctccggca tctttcacaa
     7921 aattgaacag acccatagct acctcattcg ttaacggaaa cactcagaaa gtgtagcaga
     7981 ggaacgcggc gcgttaacgc cgcgggggtg agcacaggtt taatcgcggg tttgcaccca
     8041 gaacgcgtga atcaaaccgg ggatatagcc aagcagtgtc agcagaatat taataatgaa
     8101 cgcccaaccg aaccctttac cgagcagcac gccgagcggc ggcagaatga tggtgatgac
     8161 gattctccag aaacccatat gtactcccta taagaaaatt actcattgtt taaaaagaga
     8221 ttttatctct taagcgtagt aattttagcg gagcctgcca gtttcagcgt acgcttttac
     8281 tctcatttac gctgctgatg ctggtatatg ttgcacactt agtttagagt ttacttaatt
     8341 tagaaaatac ttaaacaact atgactgaac tcgcacaatt acaggccagt gccgaacagg
     8401 cagcggcctt attgaaatca atgagccacc ctaaacgatt gctgattctg tgtatgctta
     8461 gcggttcccc cggcaccagc gcgggagagc tgacgcgcat taccggactg agtgcctctg
     8521 cgacatcaca gcatctcgct cgtatgcggg gcgaagggct tatcgactgc caacgggatg
     8581 cccaacgcat tctatattcc attaaaaatg aggcggtaaa tgccattatc gccaccctga
     8641 aaaatgtcta ttgtccgtaa ggagtcacta tggctttgac aaccatttcg ccgcatgatg
     8701 cacaagaatt aatcgcacgc ggcgcaaagt taatcgatat tcgtgatgct gatgaatatc
     8761 ttcgtgaaca tattcctgaa gcagatctgg ctccattatc cgtactggaa cagtcaggtc
     8821 ttccgcctaa attacgtcgc gagcaaatta tattccactg ccaggcaggt aaacgcacca
     8881 gtaataacgc tgataaatta gcagcgattg ccgcccccgc agaaatcttt ttactcgaag
     8941 atgggattga tggctggaaa aaagcaggat tgccagtagc ggtaaataaa tcccaaccct
     9001 taccgttaat gcgccaggtg caaatcgccg cgggtggttt aatattaatc ggcgttgtac
     9061 tgggttatac cgtaaatagc gttttcttct tattaagcgg ctttgttggt gctgggttac
     9121 tgcttgcagg aattagtggt ttttgtggaa tggcaaggtt gttagataag atgccgtgga
     9181 accaacgagc ttgagcagcg acgccggacg cgccatagca gcgacatccg gcctcagtaa
     9241 ttagatcagg aaatcgtcga gagatttacc ttctgccagc gcctgagcga ttggcttcgg
     9301 tgtacggccc tgaccggtcc aggttttagt ttcaccgtta acatcgatga atttatattt
     9361 cgccggacgc ggctggcgtt ttttaccagc gcgtggtgcc gcagcagagc tattacccaa
     9421 taactcttcc gggttaattc cgtcagcttt catcagctcc agccaggtgc taattttttc
     9481 ctggcgctct gccagttcac gctgctgttg ttcttcttct tcacgtcttt ctttagtgac
     9541 aaccctgaat ttttcgagca tttcttcaag aacgtcaatg gagaattcgc gagccatcgc
     9601 acggagggtg cgaatgttat ttaaactttg taacattacg gacataataa agaaaacctt
     9661 ttaacgccaa aacaaaaaag tatttcgtcg cgtagtgtaa gcgatcactt attatatttc
//
LOCUS       DAHHTG010000153         9608 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.156, whole genome shotgun sequence.
ACCESSION   DAHHTG010000153 DAHHTG010000000
VERSION     DAHHTG010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9608)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9608)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9608
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.156"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            154..1218
                     /gene="ulaG"
                     /locus_tag="NBI74_003933"
     CDS             154..1218
                     /gene="ulaG"
                     /locus_tag="NBI74_003933"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003856017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HCD1934200.1"
                     /translation="MSKVKSITRESWILSTFPEWGSWLNEEIEQEQVAPGTFAMWWLG
                     CTGIWLKSEGGANVCVDFWCGTGKQSHGNPLMKQGHQMQRMAGVKKLQPNLRTTPFVL
                     DPFAIRQIDAVLATHDHNDHIDVNVAAAVMQNCADDVPFIGPKTCVDLWIGWGVPKER
                     CIVVKPGDVVKVKDIEIHALDAFDRTALITLPADQKAAGVLPDGMDDRAVNYLFKTPG
                     GSLYHSGDSHYSNYYAKHGNEHQIDVALGSYGENPRGITDKMTSADILRMGEALNAKV
                     VIPFHHDIWSNFQADPQEIRVLWEMKKDRLKYGFKPFIWQVGGKFTWPLDKDNFEYHY
                     PRGFDDCFTIEPDLPFKSFL"
     gene            1326..2081
                     /gene="ulaR"
                     /locus_tag="NBI74_003934"
     CDS             1326..2081
                     /gene="ulaR"
                     /locus_tag="NBI74_003934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator UlaR"
                     /protein_id="HCD1934201.1"
                     /translation="MTEAQRHQILLEMLAQLGFVTVEKVVERLGISPATARRDINKLD
                     ESGKLKKVRNGAEAITQQRPRWTPMNLHQAQNHDEKVRIAKAASQLVNPGESVVINCG
                     STAFLLGREMCGKPVQIITNYLPLANYLIDQEHDSVIIMGGQYNKSQSITLSPQGSEN
                     SLYAGHWMFTSGKGLTAEGLYKTDMLTAMAEQKMLSVVGKLVVLVDSSKIGERAGMLF
                     SRADQIDMLITGKNANPEILQQLEAQGVSILRV"
     gene            complement(2078..2827)
                     /gene="yjfP"
                     /locus_tag="NBI74_003935"
     CDS             complement(2078..2827)
                     /gene="yjfP"
                     /locus_tag="NBI74_003935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HCD1934202.1"
                     /translation="MIEIESRELADIPVLHAYPVGQKDTPLPCVIFYHGFTSSSLVYS
                     YFAVALAQAGLRVIMPDAPDHGSRFSGDAARRLNQFWQILLQSMQEFTTLRAAIAEEN
                     WLRDDRLAVGGASMGAMTALGITARHPTVKCTASMMGSGYFTSLARSLFPPLIPETAA
                     QQNEFNNIVAPLAEWEATNHLTELGNRPLLLWHGLDDDVVPADESLRLQQALSETGRD
                     KLLTCSWQPGVRHRITPEALDAAVTFFRQHL"
     gene            3009..3338
                     /gene="bsmA"
                     /locus_tag="NBI74_003936"
     CDS             3009..3338
                     /gene="bsmA"
                     /locus_tag="NBI74_003936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313192.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm peroxide resistance protein BsmA"
                     /protein_id="HCD1934203.1"
                     /translation="MVSRKRNSIIYRFASLLLVLMLSACSALQGTPQPAPPVTDHPQE
                     IRRDQTQGLQRIGSVSTMVRGSPDDALAEIRAKAVAAKADYYVVVMVDETIVTGQWYS
                     QAILYRK"
     gene            3487..3762
                     /gene="yjfN"
                     /locus_tag="NBI74_003937"
     CDS             3487..3762
                     /gene="yjfN"
                     /locus_tag="NBI74_003937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418609.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease activator YjfN"
                     /protein_id="HCD1934204.1"
                     /translation="MKQLLASPSLQLVTYPASATAQSAEFASADCVTGLNEIGQISVS
                     NISGDPQDVERIVALKADEQGASWYRIITMYEDQQPDNWRVQAILYA"
     gene            complement(3879..5504)
                     /locus_tag="NBI74_003938"
     CDS             complement(3879..5504)
                     /locus_tag="NBI74_003938"
                     /EC_number="1.3.8.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418608.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
                     /protein_id="HCD1934205.1"
                     /translation="MHWQTHTVFNQPIPLNNSNLYLSDGALCEAVTREGAGWDSDFLA
                     SIGQQLGTAESLELGRLANVNPPELLRYDAQGRRLDDVRFHPAWHLLMQALCTNRVHN
                     LAWEEDARSGAFVARAARFMLHAQVEAGSLCPITMTFAATPLLLQMLPAPFQDWTTPL
                     LSDRYDSHLLPGGQKRGLLIGMGMTEKQGGSDVMSNTTRAERLDDGSYRLVGHKWFFS
                     VPQSDAHLVLAQTAGGLSCFFVPRFLPDGQRNAIRLERLKDKLGNRSNASCEVEFQDA
                     IGWLLGQEGEGIRLILKMGGMTRFDCALGSHAMMRRAFSLAIYHAHQRHVFGNPLIQQ
                     PLMRHVLSRMALQLEGQTALLFRLARAWDRRADAKEALWARLFTPAAKFVICKRGIPF
                     VAEAMEVLGGIGYCEESELPRLYREMPVNSIWEGSGNIMCLDVLRVLNKQAGVYDLLS
                     EAFVEVKGQDRYFDRAVRRLQQQLRKPAEELGREITHQLFLLGCGAQMLKYASPPMAQ
                     TWCKVMLDTRGGVRLSEQIQNDLLLRATGGVCL"
     gene            complement(5588..6751)
                     /locus_tag="NBI74_003939"
     CDS             complement(5588..6751)
                     /locus_tag="NBI74_003939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HCD1934206.1"
                     /translation="MLRHNVPVRRDLDRIAADNGFDFHIIDNEIYWDESRAYRFTLRQ
                     IEEQIEKPTAELHQMCLEVVDRAVKDEEILTQLAIPPLYWDVIAESWRARDPSLYGRM
                     DFAWCGNAPVKLLEYNADTPTSLYESAYFQWLWLEDARRSGVIPRDADQYNAIQERLI
                     SRFSELYSREPFYFCCCQDTDEDRSTVLYLQDCAEQAGQESRFIYIEELGLGVGGVLT
                     DLDDNVIQRAFKLYPLEWMMRDDNGPLLRKRREQWVEPLWKSILSNKGLMPLLWRFFP
                     GHPNLLASWFDGEKPQIAAGESYVRKPIYSREGGNVTIFDGQNNVVDHADGDYADEPM
                     IYQAFQPLPRFGDSYTLIGSWIVDDEACGMGIREDNTLITKDTSRFVPHYIAG"
     gene            complement(6754..7392)
                     /locus_tag="NBI74_003940"
     CDS             complement(6754..7392)
                     /locus_tag="NBI74_003940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 domain-containing protein"
                     /protein_id="HCD1934207.1"
                     /translation="MARKRKSRNNKNIGHGAISRIGRPHNPFEPRRNRYAQKYLTLAL
                     MGGAAFFVLKGCGDSGDVDNDGDGTFYATVQDCIDDGNNSDICAHGWNNAKAAFYADV
                     PKNMTQQNCQSKYENCYYDNVEQSWIPVVSGFLLSRVIRKDRDEPFVYNSGGSSFASR
                     PVWRNTSGDYSWRSGSGKKESYSSGGFTTKKASTVSRGGYGRSSSARGHWGG"
     gene            complement(7402..7800)
                     /locus_tag="NBI74_003941"
     CDS             complement(7402..7800)
                     /locus_tag="NBI74_003941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF350 domain-containing protein"
                     /protein_id="HCD1934208.1"
                     /translation="MHILDSLLAFSAYFFIGVAMVIIFLFIYSKITPHNEWQLIKNNN
                     TAAALAFSGTLLGYVIPLSSAAINAVSIPDYFAWGGIALVIQLLVFAGVRLYMPALSE
                     KIINHNTAAGMFMGTAALAGGIFNAACMTW"
     gene            complement(7818..8477)
                     /locus_tag="NBI74_003942"
     CDS             complement(7818..8477)
                     /locus_tag="NBI74_003942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjfK family protein"
                     /protein_id="HCD1934209.1"
                     /translation="MSGFFQRLFGKDNKPAIARGPLGLHLNSGFTLDTLSFRLLEDEL
                     LIALPGEEFTVAAVSHIDLGGGSQIFRYYTSGDEFLQINTTGGEDIDDIDDIKLFVYE
                     ESYGISKESHWREAINAKAMGAMTLNWQEKRWQRFFNSEEPGNIEPVYMLEKVENQNH
                     AKWEVHNFSMGYQRQVTGDTYEYLLLNGEESFNDLGEPEWLFSRALGIDIPLTSLHII
                     G"
     gene            complement(8527..9210)
                     /locus_tag="NBI74_003943"
     CDS             complement(8527..9210)
                     /locus_tag="NBI74_003943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspA/IM30 family protein"
                     /protein_id="HCD1934210.1"
                     /translation="MGILKSLFTLGKSFISQAEESIEETQGVRMLEQHIRDAKAELDK
                     AGKSRVDLLARVKLSHDKLKDLRERKASLEALSKNVNPSLINEVAEEIARLENLITAE
                     EQVLSNLEVSRDGVEKAVTATAQRIAQFEQQMEVVKATEAMQRAQQAVTTSTVGASSS
                     VSTAAESLKRLQTRQAERQARLDAAAQLEKVADGRDLDEKLAEAGIGGSNKSSAQDVL
                     ARLQRQQGE"
     gene            complement(9229..>9608)
                     /locus_tag="NBI74_003944"
     CDS             complement(9229..>9608)
                     /locus_tag="NBI74_003944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2170 family protein"
                     /protein_id="HCD1934211.1"
                     /translation="ATALQTVPEQNIDVTNNESALIIKLNDYGDLQINILFTSRQMII
                     ETFICPVSSISNPDEFNTFLLRNQKMMPLSSVGISSVQQEEYYIVFGALSLKSSLEDI
                     LLEITSLVDNALDLAEITEEYSH"
BASE COUNT         2515 a         2534 c         2273 g         2286 t
ORIGIN      
        1 attcaattta atctttctac actcaaaatc cacaataact ctcctgctaa aaagtcaaaa
       61 aatggtcatt gccttcctga aaaaatagtg tcaggattaa tcaaaaccaa tcacatattg
      121 attcgaattg aactatcacg aaggtaaatg gcgatgagta aagtgaaaag tatcacccgt
      181 gagtcctgga tcctgagcac tttcccggag tggggtagct ggctgaatga agaaattgag
      241 caagagcagg tcgcaccagg cactttcgca atgtggtggc tcggctgtac cgggatctgg
      301 ttgaaatcag aaggcggtgc caacgtttgt gttgattttt ggtgcggtac tggcaaacaa
      361 agccacggta acccgttaat gaaacagggt caccagatgc agcgcatggc tggcgtgaaa
      421 aaactgcagc caaacctgcg caccaccccg tttgttcttg atccgtttgc gattcgccag
      481 atcgacgcag tactggcgac tcacgatcac aacgatcata tcgacgttaa cgtcgctgct
      541 gccgtgatgc agaactgtgc tgatgacgta ccgttcattg gaccgaaaac ctgcgttgat
      601 ttgtggattg gctggggcgt accgaaagag cgttgcatcg tcgttaaacc gggcgacgta
      661 gtgaaggtaa aagacattga aattcatgca cttgatgctt tcgaccgcac ggcactgatc
      721 acgctgccag ccgatcaaaa agcggctggc gtactgccgg acggcatgga cgatcgcgcg
      781 gtaaactacc tgttcaaaac gccgggcggt tccctgtatc acagcggcga ttcccactac
      841 tccaactatt atgcaaaaca tggtaacgag catcagatcg acgtggcttt aggatcgtac
      901 ggagagaacc cacgtggtat caccgacaaa atgaccagtg ctgatatcct gcgtatgggc
      961 gaagcgctga atgcgaaagt agtgatcccg ttccaccacg atatctggtc aaacttccag
     1021 gccgatccgc aagagatccg cgtgctgtgg gagatgaaaa aagatcgcct gaagtatggc
     1081 ttcaagccgt ttatctggca ggtcggcggc aaatttacct ggccgctgga taaagacaac
     1141 ttcgagtacc actatccgcg tggtttcgat gattgcttca ctattgaacc agatctgccg
     1201 ttcaagtcgt tcctgtaatc tgtcgcttgt accggatagc gtggagatgc tcgcgctatc
     1261 cggcatagta tttcctggct atttcaaata tcatctaaaa aaatcaaatt ttatcggaat
     1321 agctcatgac tgaagcacaa agacatcaaa tcctcctgga aatgctcgca caattgggct
     1381 ttgtgaccgt tgagaaagtc gttgagcgtc tgggaatttc gcctgccact gcacgacgcg
     1441 atatcaataa acttgacgaa agcggcaaac tgaaaaaagt gcgcaatggc gcagaagcta
     1501 ttacccaaca gcgcccgcgc tggacgccga tgaatctgca tcaagcgcag aatcacgatg
     1561 aaaaagtacg tattgctaaa gcggcctcgc agctggttaa tccgggcgaa agcgtagtca
     1621 tcaactgcgg ctccaccgcg tttctgcttg ggcgggaaat gtgtggcaag ccagtgcaaa
     1681 tcatcactaa ttatctaccg ctggcaaatt acctgatcga tcaggaacat gacagcgtga
     1741 tcattatggg cggacagtac aacaaaagtc agtccatcac tttaagcccg cagggcagcg
     1801 aaaacagtct ctatgccggg cactggatgt ttaccagcgg caaaggactg acagcagaag
     1861 ggttgtataa aaccgatatg ctgacggcaa tggcagagca gaagatgctg agcgtggtag
     1921 ggaaactggt ggtactggtt gatagcagta agattggcga acgcgcggga atgcttttta
     1981 gccgtgccga tcaaatcgat atgctgatca ccggcaaaaa tgctaacccg gaaatcctgc
     2041 aacaactgga agcgcaaggg gtcagcattc tgcgtgttta aagatgctgg cggaaaaatg
     2101 tcacggcagc atctaacgcc tcaggcgtaa tgcggtgacg cacgcctggc tgccatgaac
     2161 aggttagcag tttatcccgt cccgtctcgc ttaaggcctg ctgcaaacgt agtgattcgt
     2221 cggcaggcac gacgtcatcg tcaaggccat gccataagag cagcggtctg ttccccaact
     2281 ctgtcagatg gtttgtcgct tcccactctg ccagcggcgc gacaatgtta ttgaattcat
     2341 tctgctgtgc tgccgtttca ggtatcagcg gtggaaacag tgaacgggcg agcgatgtaa
     2401 aatagcccga tcccatcatg ctggcggtac attttaccgt ggggtggcga gcggtaattc
     2461 ccagtgccgt catcgcgccc atcgacgcgc caccgactgc cagacggtca tcacgcagcc
     2521 agttttcttc ggctattgcc gcacgtaagg tagtgaattc ctgcatactt tgtagcaaga
     2581 tttgccagaa ttgatttaac cgccgcgctg cgtcgccact aaaacggcta ccgtgatcgg
     2641 gcgcatccgg catgatcacc cgcaaaccag cctgcgccag cgcaacggca aaatagctat
     2701 acaccagact ggacgaagta aagccgtgat aaaaaatcac acacggtaac ggggtatctt
     2761 tttgcccgac aggataagca tgaagaacgg gaatatctgc cagctcgcgt gattctattt
     2821 caatcatctg tctctccttt ttgtctggta tgacatgaag aaatccttag cacaagtttc
     2881 tgctggcggt attagttcat aatgttgaga tgtgggttac gctttcgtca cgttttcatt
     2941 cgaaaatcgc gtaccaggta acaattcggg aacattcccc caaagttaaa ttaataaggc
     3001 actacactat ggttagcagg aaacgtaata gcattattta ccggtttgcc agtttattat
     3061 tggtgttgat gttaagtgcc tgtagcgcac tgcaaggtac gccacagcca gcaccgccag
     3121 taacggacca tccgcaagag attcgccgtg accagacgca agggttacag cgaataggta
     3181 gcgtaagcac gatggttcga ggttctccgg atgacgcatt agcagaaatc agagcgaaag
     3241 ctgtcgctgc aaaagctgat tattacgttg tcgtcatggt ggacgaaacc atcgtgacag
     3301 gacagtggta ttcacaagcc attttgtacc gtaaataaat cagacaacta acatagtcag
     3361 ctttacattg ctttgcgtcc ctatgcgttt acctgtgcac aataaattac cggctggaag
     3421 ctcgggcgtt tattaccgcg atggaatgcc ctgcaacatg tggggaaacg aaaaatggag
     3481 ctgacgatga aacaattact tgcctcaccc tcgctgcaat tagtgactta tcctgcgagc
     3541 gccacggcac agtctgcaga attcgccagt gctgactgcg tgacgggcct gaatgaaata
     3601 ggtcagatct cagttagcaa tatttcaggg gatccgcagg acgtggaacg tatcgtggcg
     3661 ttaaaggccg atgaacaagg tgcgtcatgg taccgcatta ttaccatgta cgaagaccag
     3721 cagcctgata actggcgcgt acaggcgata ctgtatgcgt gagtgtcatc tacagcaaat
     3781 gaacaatgcc ggatgcggcg cgagcgcctt gtccggccta caattttaaa atatttcatt
     3841 tagttcatcg aaaccagcgt cgcatcagtc gtatacgctt acaaacacac tcctcccgtc
     3901 gcccgcagca ataaatcatt ctggatctgc tctgacaacc gtacgccgcc gcgcgtatct
     3961 aacatcacct tacaccacgt ctgcgccatt ggcggagagg catatttcaa catttgcgca
     4021 ccgcagccca gcaggaatag ctgatgagta atctctcgcc ccaattcttc agctggctta
     4081 cgcagctgct gctgtaaacg acgaaccgcg cgatcaaaat agcgatcctg ccctttcact
     4141 tccacaaatg cttccgacaa taagtcgtat acgcccgctt gcttattgag aacgcgcagc
     4201 acatctaggc acataatatt gccggaacct tcccaaatac tgtttaccgg catctcccgg
     4261 taaagccgcg gcagctcgct ctcctcgcaa taaccaatgc cgcccagcac ctccatcgct
     4321 tcggccacaa acggaatgcc acgtttgcag atcacaaatt tcgccgcagg cgtaaataaa
     4381 cgcgcccaca gagcttcttt ggcatcggcg cgccggtccc acgctcgcgc aagacgaaac
     4441 agcaacgccg tttgcccttc aagctgaagt gccatgcgac ttaagacatg acgcataagg
     4501 ggctgttgga tcaatggatt accaaaaaca tggcgttgat gtgcatgata aatcgccagc
     4561 gaaaatgcac ggcgcatcat ggcatggcta cccagggcgc aatcaaaacg cgtcatccca
     4621 cccattttca ggatcagacg aattccttcc ccttcctgcc ccaacaacca gccaatggca
     4681 tcctgaaatt ccacttcgca gctggcgtta gagcgattac ccagcttatc tttcagccgc
     4741 tcgaggcgaa tcgcgttgcg ttgcccgtca ggcaaaaagc gcggcacaaa aaagcaggac
     4801 aaaccgcccg cggtctgtgc cagcaccaga tgcgcatcgc tttgcggcac cgagaaaaac
     4861 catttatgcc ccaccagccg ataagaacca tcgtccagac gctctgcgcg ggtggtgttg
     4921 ctcatgacat cggaaccgcc ctgcttttcc gtcattccca tgccgatcaa caagccacgt
     4981 ttttgcccac ctggcaataa gtgagaatcg tagcgatcgc tcaacagcgg cgtggtccag
     5041 tcctgaaacg gcgcgggtaa catctgtaac aacaatggcg tggcggcaaa ggtcatcgtt
     5101 atcggacata acgacccagc ctcaacctgc gcatgtaaca taaaacgcgc cgcgcgcgcc
     5161 acaaatgcgc cggagcgagc gtcttcttcc caggcaagat tgtgcacccg attggtacat
     5221 agcgcctgca tcagcaggtg ccaggcgggg tgaaaacgca catcgtccag acggcgtcct
     5281 tgcgcatcgt agcgcaataa ttcaggcgga ttcacattcg ccagccgccc cagttcaagg
     5341 gattcagccg ttcctaactg ctgaccaata ctggcgagaa aatcgctatc ccagccagca
     5401 ccttcacgcg ttaccgcttc gcaaagcgcg ccatcagaca ggtataagtt gctgttattt
     5461 aatggtatag gttgattaaa aacggtgtga gtttgccagt gcactgtgtc tccctccatc
     5521 aatggcagtc accattagta tggtcactgc catggattca tgacagaaag agattgctaa
     5581 aacattctta tccagcaatg taatgcggaa cgaaacgtga ggtgtctttg gtgatcagcg
     5641 tgttatcttc acggatcccc attccgcacg cttcatcatc gacaatccag ctaccgatga
     5701 gtgtgtagct atcgccaaat cgcggcagag gttgaaacgc ctggtagatc atcggttcat
     5761 cggcgtaatc accatcagcg tggtcaacaa cgttattctg accgtcgaaa atggtgacgt
     5821 ttccgccttc gcgcgagtaa atcggtttac gcacatagct ttcgccagcg gcaatctgcg
     5881 gtttttcacc atcgaaccag gatgcaagaa ggttaggatg accagggaag aagcgccaaa
     5941 gcagcggcat tagcccttta ttactcaaga tacttttcca taacggctcc acccattgct
     6001 cacgacgctt gcgcagcagc ggaccgttat cgtcacgcat catccactcc agcggataca
     6061 gcttaaatgc acgctggatg acattatcat caagatcggt cagtacgccg ccgacgccca
     6121 aaccgagctc ttcaatgtag ataaaccgag actcctgccc tgcctgctcg gcgcagtcct
     6181 gcaagtacag cacggtactc ctgtcttcat cggtatcctg acagcagcaa aaataaaacg
     6241 gctcccggct ataaagctcg ctaaagcgtg aaatcaggcg ctcctgaatg gcattgtact
     6301 gatcggcatc gcgcggaata acgccgctgc gccgggcatc ttccagccac aaccactgga
     6361 aataagccga ctcgtacaat gaagttggcg tatcggcgtt gtactccagc aacttaaccg
     6421 gtgcattacc acaccaggca aaatccatac ggccatacaa cgaaggatcg cgtgcgcgcc
     6481 agctttcggc gatcacatcc cagtacaacg gcgggatcgc cagttgcgtc aggatctctt
     6541 catctttaac cgcacgatcc accacctcca ggcacatctg atgcagttcc gcagtcggtt
     6601 tttcgatctg ctcttcaatc tggcgcagag taaagcggta agcccgactc tcatcccaat
     6661 agatttcatt gtcgatgata tgaaagtcaa atccgttatc ggcggcaatc cggtccagat
     6721 cccgccgcac aggaacgttg tgtctcagca tgattagcct ccccaatgcc cacgggcgct
     6781 ggaagaacga ccatagccgc cgcgagaaac ggttgacgct tttttggtgg taaagccgcc
     6841 cgaagagtaa gactcttttt tgccagaacc ggagcgccag gagtaatcac cggaagtgtt
     6901 gcgccagacg gggcgggaag caaatgagga tccgccgctg ttatagacga acggctcatc
     6961 gcgatcttta cgaataaccc ggcttaacaa aaatccagaa acgaccggga tccaactctg
     7021 ttcaacattg tcgtagtaac aattttcata cttagactga caattctgct gagtcatatt
     7081 cttcggaaca tcggcataaa atgccgcttt ggcgttgttc cagccatgag cacagatatc
     7141 tgaattatta ccatcatcaa tacaatcctg cacagtcgcg taaaacgttc cgtcgccgtc
     7201 gttatcgaca tcgccactat cgccacaacc tttcaatacg aaaaaagcgg caccgcccat
     7261 tagcgccaac gttaagtatt tttgtgcgta gcgattgcgg cgcggctcaa aaggattatg
     7321 cggtctacca atgcgactga ttgccccatg tccgatattt ttgttatttc tggatttgcg
     7381 ttttctggcc atgatcgtcc attaccatgt catacaagct gcgttaaaaa taccgccagc
     7441 cagcgcggcg gttcccataa acattccggc tgcggtattg tgattaataa ttttttcgct
     7501 taatgcgggc atataaagcc tgacgccagc aaaaacgagt aattgaatca ccagagcaat
     7561 cccgccccat gcgaaatagt ctggaatact caccgcattg attgccgcac tggataaggg
     7621 gatcacgtac cccaacaatg ttccgctgaa cgccagtgcc gccgcggtgt tattgttttt
     7681 gattaactgc cattcgttgt gcggtgtaat tttggagtag ataaacagga aaataatcac
     7741 catcgccacg ccaataaaaa aataggcact aaaagcaaga agtgaatcca gtatatgcat
     7801 tgtaaacgtc cttgtaatta accaataata tgaagtgatg tcagcgggat atcgataccc
     7861 agcgcgcgcg aaaataacca ctccggctcg ccgagatcgt taaaagattc ctcaccattt
     7921 aacagcaaat attcgtaagt atctccggtc acctgccgtt ggtagcccat gctaaaatta
     7981 tgcacttccc atttggcatg gttttgattt tctacttttt ccagcatgta gacgggttcg
     8041 atatttcccg gttcttcgct gttaaaaaat cgctgccagc gtttttcctg ccagtttaag
     8101 gtcattgccc ccatcgcttt ggcgttgatc gcctcgcgcc agtgactctc tttactgata
     8161 ccgtagctct cttcatagac aaagagcttg atatcatcaa tgtcatcaat atcttcgccg
     8221 ccagtggtat tgatttgcag gaactcatcg cccgaagtgt agtaacggaa aatctggcta
     8281 cccccgccca gatcgatgtg gctgacagcg gctaccgtaa attcctcacc cggcagcgca
     8341 atcagcaatt catcttccag caaacgaaac gataatgtgt cgagcgtaaa gccactattg
     8401 agatgcagac ccagcggacc gcgagcgata gcaggcttat tatccttgcc aaacagacgc
     8461 tggaaaaaac cagacatatt ttatccttaa aatagttacc agccttgcag tgaggctggc
     8521 aaaaaattac tcgccctgtt ggcgttgcag tcttgctaat acatcctggg cgctactttt
     8581 attgctaccg ccaattccag cttccgccag cttttcatcg agatcgcgac cgtccgcgac
     8641 tttttccaac tgtgcggcag catccaggcg agcctgacgt tcggcctgac gcgtttgcag
     8701 gcgttttaag gattctgccg ctgtcgaaac gctggaagat gcgccaacgg tagaggtcgt
     8761 tacagcctgt tgtgcacgct gcatggcttc agtggcttta acgacttcca tttgctgctc
     8821 aaactgagcg atacgctgcg ctgtcgctgt aactgctttt tccacgccat cacgagagac
     8881 ttccagattc gacaacactt gctcttcagc ggtaatgaga ttctcaaggc gcgcgatttc
     8941 ttcagcaact tcgttaatca acgacggatt aacgttcttg cttaacgctt ccagactggc
     9001 tttgcgctca cgtaaatctt tcagcttatc gtgacttaat ttcacccgtg ccagcagatc
     9061 aacgcgagat tttccggctt tatcgagttc agctttagcg tcacgaatat gctgttccag
     9121 catgcgcacg ccctgggttt cttcaatgga ctcttccgcc tgggagataa acgatttccc
     9181 cagcgtaaat aaacttttta aaattcccat aaatacccgt ccttgtaatt agtgtgaata
     9241 ttcttctgta atttcagcca gatccaatgc gttatctacc agcgaggtta tctcaagcag
     9301 gatatcttca agagaagatt taagcgataa cgcaccgaaa acaatgtaat actcttcctg
     9361 ttgcacgctg gagatcccta ccgatgacaa cggcatcatc ttctgatttc ttaataagaa
     9421 ggtattaaat tcatctggat tgctgatact acttaccgga caaataaagg tttcgatgat
     9481 catttggcgg gaagtaaaaa gaatattgat ttgcaaatcg ccataatcat tcagtttaat
     9541 aattaatgca ctttcgttat ttgttacatc aatattttgt tcaggtacag tttgcagcgc
     9601 cgtcgcca
//
LOCUS       DAHHTG010000154         9536 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.157, whole genome shotgun sequence.
ACCESSION   DAHHTG010000154 DAHHTG010000000
VERSION     DAHHTG010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9536)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9536)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9536
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.157"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            complement(1..716)
                     /locus_tag="NBI74_003945"
                     /pseudo
     CDS             complement(1..716)
                     /locus_tag="NBI74_003945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     misc_feature    complement(319..435)
                     /locus_tag="NBI74_003945"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01497"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="AL1L pseudoknot; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /pseudo
                     /db_xref="RFAM:RF01497"
     gene            789..1450
                     /locus_tag="NBI74_003946"
                     /pseudo
     CDS             789..1450
                     /locus_tag="NBI74_003946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080172.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
     gene            complement(1480..2247)
                     /locus_tag="NBI74_003947"
                     /pseudo
     CDS             complement(1480..2247)
                     /locus_tag="NBI74_003947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001546148.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(2254..2634)
                     /gene="tnpB"
                     /locus_tag="NBI74_003948"
     CDS             complement(2254..2634)
                     /gene="tnpB"
                     /locus_tag="NBI74_003948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309368.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HCD1934212.1"
                     /translation="MLIPDNVWLAVKPVVMRAGTDTLTQYVQNELNAAWHDGAAFVFT
                     NKARSRIKVLRWDKHGVWLCTRRLHRGSFRWPRKGDGTWHLTPDEFHWLVCGVDWQQV
                     NGHDLAKWLPETPSAYTQNTAITQ"
     gene            complement(3583..4128)
                     /locus_tag="NBI74_003949"
     CDS             complement(3583..4128)
                     /locus_tag="NBI74_003949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021566166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dr family adhesin structural subunit"
                     /protein_id="HCD1934213.1"
                     /translation="MKIKYTMKMAAVASVMVAGIATANDNVLNGVGGADGIRLGTATA
                     SGTITNMESCTVNLTIATPDAKMNRAGMQENREITKFKVASNDCPTDTYAVWFKEIDN
                     VGNGIAQGKVNTNRFYLRMASTNGTESQKDISVGNKTGKGLSGKLANGAFEGKITLAQ
                     DTTGVPVDVYTYNLMAAVYSQ"
     gene            complement(4329..4502)
                     /locus_tag="NBI74_003950"
     CDS             complement(4329..4502)
                     /locus_tag="NBI74_003950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001539575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DraP"
                     /protein_id="HCD1934214.1"
                     /translation="MGNLPVGSRGLYKGKKRSAGMNELRHPGSTQMTRTGQAHRGGEH
                     RLNTGPPDWQTVE"
     gene            complement(4909..5352)
                     /gene="draD"
                     /locus_tag="NBI74_003951"
     CDS             complement(4909..5352)
                     /gene="draD"
                     /locus_tag="NBI74_003951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001539577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dr fimbrial tip invasin DraD"
                     /protein_id="HCD1934215.1"
                     /translation="MNGSIRKMMRVTCGMLLMVMSGVSQSAELTLESHGSRGGQLRDG
                     EKLATGRIICREAHTGFHVWMNERQVDGRAERYVVQSKDGRHELRVRTGGDGWSPVKG
                     EGGKGVSRPGQEEQVFFDVMADGNQDIAPGEYRFSVGGACVVPQE"
     gene            complement(5368..7950)
                     /gene="afaC"
                     /locus_tag="NBI74_003952"
     CDS             complement(5368..7950)
                     /gene="afaC"
                     /locus_tag="NBI74_003952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_153002854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AfaC-I/III family usher protein"
                     /protein_id="HCD1934216.1"
                     /translation="MRDTSSGRMKTGVTGLALAVMVACVMFRAESGIARTYSFDAAML
                     KGGGKGVDLTLFEEGGQLPGIYPVDIILNGSRVDSQEMAFHAERDAEGRPYLKTCLTR
                     EMLARYGVRIEEYPALFRASGEGRGASVAEEACADLTAIPQATESYQFAAQQLVLGIP
                     QVALRPQLRGIAPEALWDDGIPAFLLNWQANAGRSEYRGYGKRVTDSYWVSLQPGINI
                     GPWRVRNLTTWNRSSGQSGKWESSYIRAERGLNGIKSRLTLGEDYTPSDIFDSVPFRG
                     AMMSSDESMVPYNLREFAPVVRGIARTQARIEVRQNGYLIQSQTVAPGAFALTDLPVT
                     GSGSDLQVTVLESDGTAQVFTVPFTTPAIALREGYLKYNVTAGQYRSSDDAVEHTSLG
                     QVTAMYGLPWGLTVYGGLQGAEHYQSAALGLGWSLGRLGAVSLDTTHSRGQQKGHDYE
                     TGDTWRIRYNKSFELTGTSFTAASYQYSSDGYHTLPDVLDTWRDDRYAYRHTENRSRR
                     TTLSLSQSLGQWGYVGLNGSRDEYRDRPHRDYFGASYSTSWNNISLSVNWSRNRNSGG
                     YYGGWSRTEDSVSMWMSVPLGRWFGGTDNDISATAQMQRSTGQDTRYEAGLNGRAFDR
                     RLYWDVREQMVPGSESHADTSRLNLTWYGTYGELTGMYSYSSTMRQLNAGMSGSMVAH
                     SEGVTFGQRTGDTVALIAAPGVSGASVGGWPGVRTDFRGYTLAGYASPYQENVLTLDP
                     TTFPEDAEVPQTDSRVVPTKGAVVRAGFRTRVGGRALVSLARQDGTPLPFGAVVTVEG
                     EAGQAAGSAGVVGDRGEVYLSGLKESGKLKAQWGENSLCHADYRLPEEKGPAGIFLTR
                     TVCM"
     gene            complement(7990..8787)
                     /gene="draB"
                     /locus_tag="NBI74_003953"
     CDS             complement(7990..8787)
                     /gene="draB"
                     /locus_tag="NBI74_003953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021517431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dr fimbrial biogenesis chaperone DraB"
                     /protein_id="HCD1934217.1"
                     /translation="MKMRAVAVFTGMLTGVLSVTGLLSAGAYAAGGEGNMSASATETN
                     ARVFSLHLGATRVVYNPASSGETLTVINDQDYPMLVQSEVLSEDQKSPAPFVVTPPLF
                     RLDGQQSSRLRIVRTGGEFPSDRESLQWICVKGIPPKEDDRWAEGKDGEKKADKVSLN
                     VQLSVSSCIKLFVRPPAVKGQPDDVAGKVEWQRAGNRLKGVNPTPFYINLSALTVGGK
                     EVKEREYIAPFSSREYPLPAGASGKVQWKVITDYGGTSKQFEAELKG"
     gene            complement(9048..9353)
                     /gene="afaA"
                     /locus_tag="NBI74_003954"
     CDS             complement(9048..9353)
                     /gene="afaA"
                     /locus_tag="NBI74_003954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001545784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AFA-III adhesin operon transcriptional regulator
                     AfaA"
                     /protein_id="HCD1934218.1"
                     /translation="MRERYLYLADTPQGILMSGQVPEYQFWLLAEISPVHSEKVINAL
                     RDYLVMGYNRMEACGRHGVSPGYFSGALKRFQRVSQTVYRLVPFYFPEAGHEVHRGE"
BASE COUNT         1999 a         2981 c         2260 g         2296 t
ORIGIN      
        1 ctggtcagga cgttctgcca caaactgacg gtttacgcgg tcgcctgcgg caacggcttt
       61 ccggctgaca gtagtgcgga cctttttacc ccggagaaca ccggcaagtc ccataaccgc
      121 catgaggcgc gccactgtac atctggctac cctgattcct tcccgtaaca actgacgcca
      181 gactttacgc acaccgtata cctgatgatt cccatcgtat acgcgcagta tctctttctt
      241 cagccagtca tcgcgctgcg cacgggcact gcgtttatca ggatgatgtc gctgttgctg
      301 acagtggtaa tacgttgacg gggcaatatg tagttcactg cataccggtc cgaccccgta
      361 cagcttacgc agcttatcca gcagtggcat cactttttcc agaggcggtc gaactccgcc
      421 ttcgcaaaat aagcggaagc ctggcgaagg atatcgttac tgcggcgcag ttcacgattt
      481 tcacgttcca gctctttcag acgctgacgt tcagcggtgg tgagcccgcc atcaccgcct
      541 ccggtatccc gctcatgctg gcgtacccag acacgcagag tctccggtgt acagccaatc
      601 tttggggcaa tggaacaaat tgtcgcccat tgtgagtcat attcgccctg actttccaga
      661 accatacgga ctgcccgttg acggacttcg ggggaaaaac gagtattttt agtcatcctg
      721 tttacctctt tctcagggag tttagtctcc aggattcccg gggcggttca ggggtgacca
      781 tctcattaca tccggagcaa caccttcgtc ccttccgggg tatcctgcaa gcagatgcgt
      841 tcgcaggtta cgatcggctg ttcagtgccg aacgtgaagg cggcgcgttg acggaagcag
      901 gatgctgggc tcatgcgcgg cgcaaaatcc acgatgtata tatcagtacc aaaagcgcga
      961 cggcggaaga agcactgaaa ctaatcggcg aactgtacgc cattgagcac gaaatacgcg
     1021 ggttgccggt gtctgaacgc ctggcggtca ggcaaatgca gagtaaaccg ctactgactt
     1081 ccctgtataa gctgatgcag gagaaagaac acacgttatc gaaaaaatgc cgtctgagag
     1141 atgcgttccg gtatatcagg aagcactggg ttgcgttgtg caacttctgt gatgacggtc
     1201 tggcggaggc ggacaataac acagcggaaa gagcgcttcg tgcagtctgt ctcggaaaga
     1261 aaacgtgttc ttcggtagcg atcacggcgg cgagcgtgga gcactgctgt acgggctgat
     1321 cggtacctgt cgtctgaacg gtatcgatcc ggaagcgtat ctgcgccata ttctgagcgt
     1381 actgccggaa tggccttcca accgagttgg cgaactcctg ccatggaacg tagtactcac
     1441 caataaataa gcgtcaatac ggtgctccgt tgacgcttac agtgcagctc cggcgacacc
     1501 aaccatatcc gccattgtac tgacgggaag ctccacaccc atccgggcga aaatgtgctg
     1561 ctgacgatac aggggaagat ggtcagtata tttactgatg accacctgtg cgatgaccga
     1621 tggctcaacg gcacttttcg gaaggatgtg agccgacatt tcaccactga acaccttctg
     1681 acaacagggg caactgtact gcgggcgcac atagcggttc accacgaagt gcgcggggat
     1741 atactccagt ttttcactca ccgcatcgcg gatatggcgc agcggttcgt cacattcagg
     1801 gcaatgatca gtgaccggca ggatttcctt ttcaacacgc ggaagatggg acggtaaagg
     1861 tttacgaatc ggacgtgaac ggggcgcttt gtcgtcttct tcaacagagg gcggcagcag
     1921 tttatccagt tgcgcagtga tttcggcgat gtccacgtcc acgtccacgt ccacgttcac
     1981 gtcctcttcg aacagggaac gctgcatccc ggccagtgtt tcgcattttt taccgaagcg
     2041 ttgctgacgc accagtttca gcatttcttc cagaagctgg atacgctggc ttttggcctg
     2101 taattcccgc tctttttcag cattctcaga catggctttt tgcaccatgg caagtgccat
     2161 tgcgcgcagt ttttcgatgt catttgtcgt ggtgagagcg gagatatcca tgccgtagag
     2221 tataccactt aaggcataga tattcattat ttttcactga gttatcgctg tattttgcgt
     2281 atatgcggaa ggtgtttccg ggagccattt agccaggtcg tgaccattga cctgttgcca
     2341 gtcaacgcca cagacgagcc agtgaaactc atccggtgtg agatgccatg tgccatcacc
     2401 tttccggggc cagcggaagc ttccccggtg cagtcgtcgg gtacacagcc atacaccatg
     2461 tttgtcccac cggagaactt ttatccggga gcgggctttg ttggtaaaga caaaagcagc
     2521 tccgtcatgc catgctgcgt taagttcgtt ctgaacgtac tgtgtcaggg tgtcggtacc
     2581 ggcacgcata acgacgggtt tcacggccag ccagacgtta tcaggtatca gcatggcgaa
     2641 gcgccctgaa tacatcagcc agttgcgctg cccggcagca cagtcggata ccaccgggca
     2701 gatataacga aaccggttcg tcagcggggg cagtcgcagt tgttccccga ccctccggaa
     2761 gtccggatac gctgaccggc atgaaagcgg gtccatgcgc cttccattcg gcccgggtga
     2821 tatctttcgg ccgtagcgca tggatttgga tttaagggtg atatcgtgct tttccagttc
     2881 gccatccggg aaagtccact ggcccgctat gcgccaacat gttgtctttt ttgctcaagc
     2941 gtccattgtg tatccggcat tgtcgttttc tgccatggtt tacagacatg catcaatcct
     3001 aaagttggga acttttctgt gaagatgagt tcaccgctac gtcactgaag atgtgttcac
     3061 cgtaatgttt caggtcacct gaacgtttcg taagactagc gtgacttgct cattgttgtc
     3121 ggagggttca ttagcgcgtt gggcttgaca acatgagagg gacgttcggg cctttcgcat
     3181 gccacataat gaacgtgcgc cgtaaggact gcgatatttg tccatatagc gcacaactac
     3241 cgtgcgtcgg ggtggtcggg ctttttatta gtcatcgcgg gttgtcaaag ccgttcccag
     3301 tattgagttc ttgtgttgcc cgttcgatta tcgtgaccct ggcgtcggtg tgcggtcgtc
     3361 gtccgtgagg cctgacaacc aggtgtagca gtacacttgg gccctccttt tggccctgtc
     3421 cattgccgtt tccggcggat tgtcatggag accgggcgat cgcagcccgc cgcccgtagc
     3481 ggcagttgtt cagccacttg gttgcctgcg acgaggtcga ttcccgcgag gtaagcaaga
     3541 ccgccggcgg gcggcggtcg gtgttcggta cggttgtgag cattattggc tgtacactgc
     3601 ggccatgagg ttgtatgtat agacgtcgac gggtacgccg gtcgtgtctt gggccaaagt
     3661 tattttcccc tcgaatgcgc cattggccag cttgccgctc aggcctttgc ctgttttgtt
     3721 ccctacgctt atgtcctttt ggctttccgt accgttcgtc gatgccatcc gtaggtaaaa
     3781 tctgtttgta ttcactttgc cctgtgcgat cccgttgcct acgttatcga tctctttaaa
     3841 ccatacagca taggtgtcgg tagggcaatc gttgctcgct accttaaatt tagtgatttc
     3901 gcggttttct tgcatccctg cccggttcat cttagcgtca ggggtagcga ttgtcagatt
     3961 tactgtgcag ctttccatgt tggtgatcgt cccgctcgcg gttgcggtgc ctagacggat
     4021 gccatcagcg ccccccaccc cgttgagtac gttgtcattc gcggtcgcga ttccggcgac
     4081 catgacgctg gcaactgccg ccattttcat tgtatatttt attttcatat ttttttgcct
     4141 tcgttgtgct tatgggcaag ccagttgtgt tctgttaatc catcccagaa tcagtaagct
     4201 gtcttccccc aaattaacca ccgttcacag atgtctgcac ccgcagcaga gactgttcat
     4261 tgcatataag taagtttctg actgcagggc ttgctcataa agccgcagac aacgtgcacg
     4321 gtgatgcctt attccacggt ctgccagtcc ggtggccccg tgttcagccg gtgttcgcca
     4381 ccgcgatgcg cctgaccagt gcgcgtcatc tgtgtgcttc ccggatgacg taattcattc
     4441 ataccggcgc tgcgtttttt ccctttatat aaaccacgac ttcccactgg cagattaccc
     4501 ataataaaac ctccacctta tttctgattt aaatgattct gactgtttaa attaaatccg
     4561 gatggccgat ttatatttat gtaataaacc gccagcacgg attaactgcc tgtgttaatc
     4621 agctttgtgc tttccttctc tggtctttta tttttccttt attctttatt ctttcctgta
     4681 ttccctgcgt gctccctccg ggttgcaggt atccctccgc ctcttctgcg gaatacaagt
     4741 cactgccatc cgtgaattcg tgtttatttc atttcagtta atttaatccg gtgaaataaa
     4801 tactctctgt gttttctgtt ttgtttttgt ttttgttttt gtttttgttt tatgctgtgt
     4861 tgtgcctttg tcttttcttc tgtttttatt ctgtttttat tctgcgcttt attcctgtgg
     4921 cactacacag gctccgccaa ccgaaaaccg gtattcacca ggagcaatgt cctgatttcc
     4981 gtccgccatc acatcaaaaa aaacctgctc ctcctgaccg ggcctcgaca cccccttccc
     5041 gccttctccc ttcaccggcg accagccatc tcctcctgtc ctgacacgaa gctcatgacg
     5101 accatcctta ctctgcacca catagcgctc cgccctgccg tccacctgac gctcattcat
     5161 ccatacatga aaacccgtgt gcgcctcccg gcagataatc cgccccgtcg ccagcttctc
     5221 tccatcacgt aactgcccgc cgcgactgcc gtgactctcc agtgtcagtt ccgctgactg
     5281 cgacacacca ctcatgacca tcagtaacat cccgcaggtg acacgcatca tcttccttat
     5341 actcccgttc atctccggct cctcccgtca catacacacc gtacgggtca gaaatatccc
     5401 cgcaggaccc ttctcttccg gaagacggta atccgcatgg cacagactgt tctctcccca
     5461 ctgcgccttc agcttaccgc tttccttcag cccgctcagg tacacctcac cacggtctcc
     5521 caccacaccg gctgagcccg cagcctgacc cgcttcgccc tcaactgtca ccaccgcacc
     5581 aaacggcaga ggcgttccgt cctgacgggc cagactcacc agcgcacgac cacccacacg
     5641 ggtcctgaat ccggcccgga ccactgcccc cttcgtcggc accacacgac tgtccgtctg
     5701 cggcacttcc gcatcctccg ggaacgtcgt cgggtccagt gtcagcacgt tctcctggta
     5761 tggcgaggca taaccggcca gcgtataccc ccggaaatcc gttctcacac ccggccagcc
     5821 ccccacagac gcaccactca cgcccggtgc cgcaatcagt gccaccgtat ccccggtccg
     5881 ctgaccaaag gtgaccccct cactgtgggc caccatgctg ccggacatcc cggcgttcag
     5941 ctggcgcatc gtgctgctgt aactgtacat ccccgtcagt tcaccatatg tcccgtacca
     6001 cgtcaggttc agacgactgg tgtcagcatg gctctcactg cccggcacca tctgctcacg
     6061 gacatcccag tacagccggc ggtcaaatgc ccgtccgttc agcccggcct cataccgggt
     6121 atcctgtccc gtggaacgct gcatctgcgc cgtggcactg atgtcgttat ccgtcccccc
     6181 aaaccagcgt cccagcggca cactcatcca catactgaca ctgtcttccg tacgcgacca
     6241 gccaccgtaa tagccgccgc tgttgcggtt gcgtgaccag ttaaccgaca gcgagatatt
     6301 gttccaggac gtactgtatg acgcgccaaa ataatcacgg tgcggtctgt cacggtactc
     6361 atcccggctg ccgttcagtc ccacatagcc ccactgaccc agggactgac tcagactcag
     6421 cgtggtacgg cgactccggt tctccgtgtg acggtatgcg taccggtcat cacgccaggt
     6481 gtccagcacg tccggcagcg tatggtaacc atccgatgag tactgataac tcgctgccgt
     6541 aaaactcgtc cccgtcagct caaacgactt gttataacgg atacgccagg tgtcaccggt
     6601 ctcataatca tgtcccttct gctgcccccg ggagtgcgtc gtgtccagcg acaccgcccc
     6661 cagacgcccc agtgaccagc caagccccag agccgcagac tggtaatgct ccgctccctg
     6721 aagccccccg tacaccgtca gcccccacgg cagaccgtac atggccgtca cctgtcccag
     6781 cgacgtgtgc tcaaccgcat catccgatga acggtactga cccgccgtga cgttgtactt
     6841 caggtacccc tcacgcagcg caatggccgg cgtggtgaac ggcaccgtga aaacctgcgc
     6901 cgtcccgtct gattccagca ccgtcacctg caggtcactg ccggaccccg tcaccggcag
     6961 gtccgtcagg gcaaatgccc ccggcgccac cgtctgactc tgtatcagat acccgttctg
     7021 acgcacctct atccgcgcct gtgtccgggc aatgccacgt acaaccggcg caaattcacg
     7081 caggttataa ggcaccatgc tctcatcaga actcatcatc gcaccccgga aaggcacact
     7141 gtcaaaaatg tctgacggcg tgtaatcctc acccagcgtc aggcgactct ttatcccgtt
     7201 cagcccccgc tcagcacgta tgtatgaact ctcccatttt cccgactgac cggatgacct
     7261 gttccaggtg gtcaggttcc taacacgcca gggtccaatg ttgattcccg gctgcagact
     7321 gacccagtaa ctgtccgtga cacgcttccc gtaaccccgg tactcactgc gccccgcatt
     7381 cgcctgccag ttcagcagaa aagccggaat gccatcatcc cataacgcct ccggcgcaat
     7441 ccccctcagc tgcggacgca gtgccacctg agggataccc agaaccagct gctgggcagc
     7501 aaactgataa ctctccgtgg cctgcggtat cgccgtcagg tcagcacagg cctcctccgc
     7561 cacggaggca ccacgaccct ctccggatgc acggaacaac gccggatatt cctcaatcct
     7621 gaccccgtaa cgcgccagca tctcacgggt cagacaggtc ttcagataag gcctgccctc
     7681 cgcgtccctc tccgcgtgaa aggccatctc ctgtgaatcc acacgggaac cattcaggat
     7741 aatgtcaacc ggataaatgc cgggtaactg cccaccctcc tcaaacaggg tcaggtccac
     7801 ccccttcccg ccacctttca gcatggccgc atcaaaggag taggtgcgcg caataccact
     7861 ctccgcacga aacatcacac aggccaccat cacagccagc gccagccctg tcacccccgt
     7921 tttcatccgc cctgaagaag tatcacgcat ctgtcagccc gccacttacc ctgtcatcac
     7981 cttatgtatt caacccttca gctctgcctc aaactgctta ctggtcccgc cgtaatccgt
     8041 tatcaccttc cactgaacct tacccgatgc ccccgcaggc agcggatatt cacgggagga
     8101 aaacggcgca atatattcac gctccttcac ttccttaccc cccaccgtca gcgcggacag
     8161 gttgatgtaa aacggcgtcg ggttaacccc cttcagcctg ttgccggctc tctgccactc
     8221 caccttgccg gccacatcat ccggttgccc cttcaccgcc ggcggacgaa caaacagctt
     8281 gatgcagctg ctcactgaaa gctgtacatt cagggagact ttgtcagcct tcttctcccc
     8341 gtccttccct tccgcccacc tgtcatcctc cttcggcgga atgcctttca cgcaaatcca
     8401 ctgcagactc tcacggtctg acggaaactc cccgcccgtc ctgacaatac gcagacgact
     8461 cgactgctga ccatcaagac ggaacaacgg tggtgtcacc acaaaaggcg ccggactctt
     8521 ctggtcctca ctcagcacct ccgactgcac cagcatcgga tagtcctggt cattaatcac
     8581 cgtcagcgtc tcccccgacg aggccgggtt gtaaaccacc cgcgtggccc ccagatgcag
     8641 cgagaatact ctggcgtttg tctccgtcgc ggatgcagac atattccctt ctcccccggc
     8701 ggcatatgcc cccgctgaca gcaaacccgt cactgataac actcccgtca gcatgccagt
     8761 gaacaccgcc acagcccgca ttttcatact ccctccggaa cagacagata cacgttgtct
     8821 tttatatatt cattcaccat ccccacccgg aactgactct gaccggactg catgcagtca
     8881 ccgcattcgt gccgggtggc gaaacggatg gtgaaactga tgttaatatt cagtgcttaa
     8941 tgcttaatgc ttaatgctta atgcttaatg cttaatgctt aacagaaata gctttaatta
     9001 gcgtaataat atcaaccgca cttattaaac ccgctcacgc cctttcatca ctctcccctg
     9061 tgaacttcat gacccgcctc cgggaaataa aaaggcacca gcctgtatac cgtctgactg
     9121 acccgctgaa accgcttcag tgcaccagaa aaatatcccg gcgacacacc atgacgcccg
     9181 caggcctcca tacggttata tcccattacc agataatccc tcagcgcatt aataaccttc
     9241 tcactgtgta ccggcgatat ctcagccagt aaccagaact gatattccgg cacctgaccg
     9301 gacatcagta tcccctgagg ggtgtcagca agatacagat atcgctccct catctctgat
     9361 tcactccatg cacacgaata acatgcaggg aatgaaacct tttcacttca gattcaacag
     9421 gatacctgtc tccccctgcg tcaacggcac aaatactatc atggtgtttc ttacttaaaa
     9481 atatacattg cacgatcttt ttatctacaa aatcacaaaa taccagaatc atataa
//
LOCUS       DAHHTG010000155         9501 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.158, whole genome shotgun sequence.
ACCESSION   DAHHTG010000155 DAHHTG010000000
VERSION     DAHHTG010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9501)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9501)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9501
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.158"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            <1..420
                     /locus_tag="NBI74_003955"
     CDS             <1..420
                     /locus_tag="NBI74_003955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HCD1934219.1"
                     /translation="DVYTCEIVGYAMGERMTKELTGKALFMALRSQRPPAGLIHHSDR
                     GSQYCAYDYRVIQEQFGLKTSMSRKGNCYDNAPMESFWGTLKNESLSHYRFNNRDEAI
                     SVIREYIEIFYNRQRRHSRLGNISPAAFREKYHQMAA"
     gene            465..887
                     /locus_tag="NBI74_003956"
                     /pseudo
     CDS             465..887
                     /locus_tag="NBI74_003956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000291745.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1303..2835
                     /locus_tag="NBI74_003957"
                     /pseudo
     CDS             1303..2835
                     /locus_tag="NBI74_003957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013317439.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(2869..3378)
                     /locus_tag="NBI74_003958"
                     /pseudo
     CDS             complement(2869..3378)
                     /locus_tag="NBI74_003958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000544017.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
     gene            3398..3720
                     /locus_tag="NBI74_003959"
                     /pseudo
     CDS             3398..3720
                     /locus_tag="NBI74_003959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000555401.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            complement(4298..4513)
                     /locus_tag="NBI74_003960"
     CDS             complement(4298..4513)
                     /locus_tag="NBI74_003960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001622039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1934220.1"
                     /translation="MWLLIFWLSSPFNVSSGDARQPTLAVHSQAFHTERACRDALHAI
                     KKENVGEFSLRGVCVSDGNTKEEAESS"
     gene            complement(4517..4885)
                     /locus_tag="NBI74_003961"
     CDS             complement(4517..4885)
                     /locus_tag="NBI74_003961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001456887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1934221.1"
                     /translation="MKDNSTTATPEAWKMRFLANGTSGTWFYTDDITVTDAWLRAHPD
                     DVEVIPLCRIPPRQHSHEQHIEELRAALARMLHMHHMMLETTHYRENITDARCIREMH
                     EAPLQALNILARYPHKKDEV"
     gene            complement(5176..5418)
                     /locus_tag="NBI74_003962"
     CDS             complement(5176..5418)
                     /locus_tag="NBI74_003962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1934222.1"
                     /translation="MKDEIHSTSINPDVVEQFLALMNTHCKSSEEALAMLSTLCYNLM
                     VTCGIHSMDSQMLNGHGISMQLQGIDIAERPGKNIH"
     gene            5903..6062
                     /locus_tag="NBI74_003963"
                     /pseudo
     CDS             5903..6062
                     /locus_tag="NBI74_003963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001066419.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(6280..8370)
                     /gene="iha"
                     /locus_tag="NBI74_003964"
     CDS             complement(6280..8370)
                     /gene="iha"
                     /locus_tag="NBI74_003964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional siderophore receptor/adhesin Iha"
                     /protein_id="HCD1934223.1"
                     /translation="MRITTLASVVIPCLGFSASSIAAAEDVMIVSASGYEKKLTNAAA
                     SVSVISQEELQSSQYHDLAEALRSVEGVDVESGTGKTGGLEISIRGMPASYTLILIDG
                     VRQGGSSDVTPNGFSAMNTGFMPPLAAIERIEVIRGPMSTLYGSDAMGGVVNIITRKN
                     ADKWLSSVNAGLNLQESNKWGNSSQFNFWSSGPLVDDSVSLQVRGSTQQRQGSSVTSL
                     SDTAATRIPYPTESQNYNLGARLDWKASAQDVLWFDMDTTRQRYDNRDGQLGSLTGGY
                     DRTLRYERNKISAGYDHTFTFGTWKSYLNWNETENKGRELVRSVLKRDKWGLAGQPRE
                     LKESNLILNSLLLTPLGESHLVTVGGEFQSSSMKDGVVLASTGETFRQKSWSVFAEDE
                     WHLTDALALTVGSRYEHHEQFGGHFSPRAYLVWDVADAWTLKGGVTTGYKAPRMGQLH
                     KGISGVSGQGKTNLLGNPDLKPEESVSYEAGVYYDNPAGLNANVTGFMTDFSNKIVSY
                     SINDNTNSYVNSGKARLHGVEFAGTLPLWSEDVTLSLNYTWTRSEQRDGDNKGAPLSY
                     TPEHMVNAKLNWQITEEVASWLGARYRGKTPRFTQNYSSLSAVQKKVYDEKGEYLKAW
                     TVVDAGLSWKMTDALTLNAAVNNLLNKDYSDVSLYSAGKSTLYAGDYFQTGSSTTGYV
                     IPERNYWMSLNYQF"
     gene            complement(8870..>9501)
                     /locus_tag="NBI74_003965"
     CDS             complement(8870..>9501)
                     /locus_tag="NBI74_003965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HCD1934224.1"
                     /translation="YRTMRQNALLLERKPAVPPSKRAHTGRVAVKESNQRWCSDGFEF
                     RCDNGEKLRVTFALDCCDREALHWAVTTGGFDSETVQDVMLGAVERRFGSELPASPVE
                     WLTDNGSCYRANETRQFARMLGLEPKNTAVRSPESNGIAESFVKTIKRDYISIMPKPD
                     GLTAAKNLAEAFEHYNEWHPHSALGYRSPREYLRQWASDGLSDNRCLEI"
BASE COUNT         2460 a         2380 c         2255 g         2406 t
ORIGIN      
        1 gatgtttata cgtgcgaaat tgtcggctac gccatgggag agcgcatgac aaaagagctg
       61 acaggtaaag ccctgtttat ggcgctcagg agccagcgcc cacctgccgg gctaatccac
      121 cactctgatc gaggttcaca gtactgcgca tacgattacc gggtcataca ggagcagttt
      181 ggtctgaaaa catcaatgtc gcgtaaaggt aactgttacg acaacgctcc gatggaaagc
      241 ttctggggaa cgctgaaaaa tgagagcctg agccactatc gttttaataa ccgggatgaa
      301 gccatctcag taatacggga atacattgag attttctaca atcgtcagcg tcgtcactct
      361 cgtctgggga atatctcccc ggcagccttc agggaaaaat atcatcagat ggctgcttaa
      421 aaaaagaaca aatggtagtg tccgctattg ccagtacacc tcaaactgag ttctcatggt
      481 tccgatacgg acagtataac gaaacgggta tttacatctc gatcgagaag accattatca
      541 ttacaatcac cgtttctggt gggtgcttta aaaaagaata cagcattgtc tcccacatgc
      601 tggtcactga caccataaca gaggccaccc tgtacgaaaa cgggctgtat acgcgccaca
      661 tcagactcag tcgcccagta tctgacagca aatacccctt gccacccgga tctcagctca
      721 ttaaaaatat gaccttacga ctgcgcctgc aggatcagca ggaagaaaca tccgtgacat
      781 tatttcaggg aaaaatgagt accgacggaa ataactattc catcatcaaa ggtaaagtaa
      841 gctcagtact tctgttatta aaaatgctgc agattaatca tgcctgatct ctgtcccggt
      901 attttagccc gaggtatatt ctgaaatatc attcatcatg accggcgttt atacaaacgc
      961 cagtcattcg ctaacagctt caccgttcct tcccccatcc aggttccgca tgagcgtgtt
     1021 gtaatgttca taagcgtatt atcaggattg tctggaaaat tcccgatact ttggactatt
     1081 actcctgaac aactgtaagc gtacagcctg aaccgtctgg tcagaatctg acgaattaga
     1141 caaagtggtg tccaccaaat aagtagtggg aaccaaagtg tcagatatgc agaaaaatgt
     1201 gactcccggc aggcgaaaag gctgccctaa ttatcctccc gaatttaaac agcagctcgt
     1261 tgggggatgc ggacgatttc ctggactctc aggagataaa acatgtttac tgaagaagaa
     1321 aaaatccgcg ccattgagct ttatttcaaa tatggtaaga agctggctcc cgtcgtacgc
     1381 gaactgggct acccttcgaa aagaaatctg cggcgttgga tccgctcatg ggaagcaggt
     1441 ggtggcgcaa aagaatccat tcgtcataag caccgttact ccgatgagca aaaacaggtt
     1501 gccgttgaac attatcttaa ccacggctgc tgcctggcgt ttaccagcag agccctgggt
     1561 tatccctgca ctgatgtact cgctcgctgg gttaatgaac tttatcccga cagacgacgt
     1621 atatttacca gtaaggccaa cccggttgcg ccctttgaac cagaagtcaa acgtcaggct
     1681 gtcatggcac tctgtacacg acaagtatca gccagtgaga tcgccaggcg tattggtgtc
     1741 agtcgtgcgg tattgtataa atagaaagat gaaattatcg gcaacagtgc ttaccagact
     1801 atgcgtaaac ataacgaacc ttccctggag gcagaacgcg atgcgttgcg ggaggaagtc
     1861 gcccgactga atcaggaaat acgccgccgg cagatggagc tggatattct gaaaaaggcg
     1921 gaggaaatca taaaaaaaga cccgggcatc agtatcagtc acctgaacaa cagagaaaaa
     1981 acaaagatcg ctgatgccct gaggcaaaca tatcccctga cagaattact tcatgttctg
     2041 ggccttaccc gtagcagtta tttttatcac cgggctgcac tgaaagccgg tgataaatac
     2101 gccacgatac gtacgatgct gacagatata tttaacagta attaccagtg ttatggctat
     2161 cgtcgcctgc atgcgatgct caggcatgag ggggggcggc tatcagaaaa ggttgtacgc
     2221 agacttatgg tggaggaaca gcttgtcgtc agccgtaacc gtcgtcgtcg ctacagctca
     2281 tattgcggag aaatcggacc ggctccggat aaccttatcg ccagagattt taaggcgtag
     2341 caacctaatc agaaatggct gacagatatc acggagttcc aactccctgc aggtaaagtc
     2401 tggctgtcac cggtggtgga ctgcttcgat ggaaaagtta ttagctggtc tctcagtaca
     2461 cgccccgatg ctgagctggt caacactatg ctggatagcg ctgtcgaaac gttaaatgct
     2521 ggcgaacgac cggtgataca cagtgacaga ggtgggcatt atcgctggcc aggctggctg
     2581 gaaagagtga atgcagcagg tcttattcgc tcaatgtccc gtaaaggatg ttcacctgat
     2641 aatgccgcat gcgaaggttt tttcggcaga ctgaaaacgg aaatgtatta tgggcgtaaa
     2701 tggtcgggca tcacgccaga aaagttcatg cagcatgtag atgcttacat cagatggtat
     2761 aacgagcggc gtataaaatt atcgctgggt gcagtcagcc ctgaaatgta ccgccaacaa
     2821 tgtgggctgg cataataaag cagtctagga aatcgtccgc atccccaatt caggggcggt
     2881 tttttcttta cggtttttcg ccataaagac tattactatc agactgacaa gactacccgc
     2941 gaaagccggg agttccgcgc caagattaac agccaccaga tactggggaa ttaatgtact
     3001 gacaccacac atcagggtaa ttagtccaac gcctcgtata gtcttcatac caccaccgat
     3061 aatcgcgacg atgacgaaag gaagcagaat gttgaacagt gccagttgaa gaataattgt
     3121 cccgctgagt gtggttacag gtaaattaac ttgttcagca agtacagaga caggtatacc
     3181 tactgcccca aacgctgtag ggactgtatt agcaaccagt gatgcaatcg ctgcttttag
     3241 tgggttaaag cccagagcaa tcagtatacc aatggggatt gctaccgctg taccgtatcc
     3301 ggctgccgcc tcaagaaatc caccaaaaca ccatgagata agaagtacct ggatacgttt
     3361 gtcatcacta acactcgctc aatcatttgg tctgctatct caacagattg ctttcaacat
     3421 tattgtcaga atctggagca gattgaagat cgcatacttc aactcgatca ggaaatatct
     3481 cgcctgttac ctgagtgatc tctgtgcaat ctggtctgcc agttgcaggc tcttaaaggt
     3541 gttggtcaac taatcgcaat aaccctggtt gctgaactgg gtgatttttc gcgattttcc
     3601 aatcctgaac agctcatggc ttttctggga ctggtgccag gtgaatattc cagcggaaac
     3661 agcattcgcc ccagaggaat aacaaaagtt ggaaacagcg aactgagaca tctgctttac
     3721 ggatatcaaa taaattcaga ttatttaaca gtaaaacccc acttatgtga tgaatctcat
     3781 aaaaaagcaa agtgaagtaa taatatccaa tttatccctc attccttaag taagcgtaca
     3841 ctggccgcgt acaccatggc ataatgatat gttgttttac tgatatttcc ggctggtaaa
     3901 atccccgacg gcccggctga agctggtgca ggaaagcggg ttaattgtca ctacgcggga
     3961 taacctgtct ggctggatac aacgtattgc cacagatatg cagcaactgc gtcagtccgg
     4021 aatgtcagtc atctccgggg atgttggggt ctcaggcaca ctgaccggcc ccccgtgttg
     4081 tttccgggta ctgtttttga tttatgcaac aaagtattgc agcgtcgaat atgagctatg
     4141 gccagatttt tgttaatcgt ggttgttgca acatgtaagg cgaatgacca cactattctg
     4201 gcacgctcgt aatcacgctg aaattccgct tcctgtgctg ccgtgaagag ttccgaccag
     4261 gacatacggc gaagtgtatg tctgtcagta atatccgtca tgagctctca gcttcctcct
     4321 tcgtatttcc gtcagaaaca caaacgcccc gcagagaaaa ttcgccaaca ttctcctttt
     4381 taatggcgtg caatgcatcg cgacaggccc gttcggtatg aaatgcctgg ctgtgcaccg
     4441 ccagtgtggg ctgccgggca tcaccggaag agacattaaa gggagatgaa agccagaaaa
     4501 tcagtagcca catgcgtcac acctcgtcct ttttatgcgg gtatctggcc agaatattga
     4561 gcgcctgtaa tggagcttca tgcatttcac ggatacaacg ggcatcggtg atgttttccc
     4621 tgtaatgcgt ggtttccagc atcatatgat gcatatgcag catccgggcc agggctgcac
     4681 ggagttcttc aatatgttgt tcatgggaat gctgacgggg tggtatccgg cagagaggga
     4741 taacctccac atcatccggg tgcgcgcgaa gccacgcgtc agtgactgta atgtcgtcag
     4801 tgtaaaacca tgtgccggat gtcccgttgg ccagaaaacg cattttccag gcttctgggg
     4861 tggcggttgt gctgttatct ttcatggtgc caccactcat tcaacaggct taatgctgtc
     4921 caggcgcagt ctgctcagac tgtcgtggct gccacagcgg cgtcctgtac ttttgctgta
     4981 gcactcctgc cccctgatac accatgccgt tggcgtttcg tagaggctga cacgtcgggt
     5041 tttatcttta ctggctagaa gcccggtgtg agtttttgtc tttttcatcg gtggggtaca
     5101 aacgcagaaa ttttgttgtc atcagttttt gcctgcagag gcctgaaaag acagcgtttt
     5161 cagaccctgt tcaggttagt gaatattctt acctggcctt tcggcaatat caatcccctg
     5221 aagttgcatg gatatcccat gcccattgag catctggctg tccatggaat ggataccgca
     5281 tgtcaccatc aggttgtaac aaagcgtact cagcatggcg agcgcctctt ctgaggattt
     5341 acaatgtgtg ttcataaggg ccagaaactg ctcaacaaca tccgggttga tgctggtact
     5401 gtgtatttca tccttcatta tgtgtccttg ttttttatta ttttgcattt cagaaaataa
     5461 cacgatgcat gtaacccaga aaaccgatat gtgcacgcgt agtcactttc tggcagattc
     5521 cggtctctga aacaaaatac gaggtggcat gaagcggatt gtggcggaca gggaaagtga
     5581 tttgattccg gggaaggcgc tgtgcagcag tcttatccgg cagtttaaag gggtggacgg
     5641 agaccaatga ggcactggag cagattcagt gcagaggtga catgatttat caaacttaat
     5701 tgtgacaaaa tggcgattga gaaggtgcaa ttgacttatc cggattcggt cgtggcgagg
     5761 tggtgaccct gcattctgct aaccgggtta tgagccagga gtcagcgata gacaaaggga
     5821 cttatctgac cgggcccccg gaaaaatggc gggagagaat gataccttcc tgctgacgag
     5881 accggacttt gagatgacga gcgatagtgc ggcagtgatg tacagcctga tcggtagctg
     5941 caaaacttaa cggaatcgag ccggaaacgt ggctacgcca cgtgatcagt gtaatcaaca
     6001 cctggcctgc caaccgcatg aaagagttgt tgccctggaa cgtcactctc tctgtacact
     6061 aatttttacc ccacgtcctt cacggtgcgc ttacactctg gacaagtatg taaatgataa
     6121 gttaaaaata atccgtgcat ttcctgtgac gatattagtt gttggtaacg aaatctcatt
     6181 agcggatcgg aaatgcactt aacctaatac gttgccactg ttccgccagg agttacagtg
     6241 gcaacgtatt ctaccgtcag tgatagcgtt ttgttattat cagaactgat agttcagcga
     6301 catccagtaa tttcgctcag gtatcacata tcctgttgtt gatgatcccg tctggaagta
     6361 atcaccggca tacagcgtac tcttaccggc actgtacagg ctcacgtcac tgtaatcctt
     6421 gttgagcagg ttattcaccg cagcattcag cgtcagggca tccgtcatct tccacgacag
     6481 acctgcatcc accaccgtcc aggctttcag gtattctcct ttctcatcat acactttctt
     6541 ctgtacagcg ctcagtgacg aataattctg ggtgaaacgt ggtgttttcc cgcgataacg
     6601 ggcacccagc catgatgcca cctcttcggt gatctgccag ttcagtttcg cattcaccat
     6661 gtgttcaggg gtataactca gcggcgcacc tttgttatca ccatcacgtt gttcacttcg
     6721 ggtccaggtg taattcagtg acagcgtgac atcctctgac cacagcggca atgtgccggc
     6781 aaattccaca ccatgcaacc gggcctttcc gctgtttaca tagctattgg tgttatcatt
     6841 tatggaataa gagacaatct tgttggagaa gtcagtcata aaacctgtga cattggcatt
     6901 cagaccggcg gggttatcgt aatacacccc agcctcataa ctgacgctct cttccggctt
     6961 caggtcgggg ttaccaagta gatttgtttt tccctgcccg gatacaccac taatcccttt
     7021 atgtagctgc cccattctgg gtgccttata tcccgtggtc acaccgcctt tcagcgtcca
     7081 ggcatctgcc acatcccaga ccagatatgc acgcggactg aagtgtcccc cgaattgctc
     7141 atgatgttca tagcggctgc ccacagtcag cgcaagtgca tccgtgagat gccactcatc
     7201 ctcagcaaat accgaccagc ttttctgccg gaaagtttca cctgtgctgg caaggacaac
     7261 tccgtctttc atggacgagc tctgaaactc gccccccacc gtaaccagat gagattctcc
     7321 cagaggggta agcagtaatg aattcaggat aaggttcgat tccttaagct cccgcggctg
     7381 accggcaagc ccccatttgt cgcgcttcag tacactgcgt acgagctcac gacctttatt
     7441 ttctgtctcg ttccagttca gatacgattt ccatgttccg aaggtgaaag tatgatcata
     7501 gccagctgaa attttgtttc gctcatagcg cagggtccgg tcatatcccc ccgtcagact
     7561 ccccagttgc ccatcccggt tatcataacg ctgccgggtg gtatccatat caaaccagag
     7621 cacatcctgc gccgacgcct tccagtcaag acgtgcacca agattataat tctgtgactc
     7681 cgtgggataa ggaatacgcg tggctgctgt atcgctcagt gatgtgaccg atgagccctg
     7741 acgctgttgt gtgctaccgc gtacctgcag gctgacagaa tcatccacaa ggggaccact
     7801 gctccagaaa ttaaactggc tgctgttacc ccatttgttg ctttcctgca gattcagccc
     7861 tgcattgacg gaagagagcc atttgtctgc attctttctg gtaatgatat tcaccacacc
     7921 gcccatcgca tcagagccat acagtgtgga catcggcccc ctgataacct caatacgctc
     7981 aatggcggcc agagggggca tgaacccggt attcatggca gaaaaaccgt tgggagtcac
     8041 gtcactgctt ccgccctgac gaacaccatc aatcagtatc agcgtgtaac tggctggcat
     8101 tcctcggatg ctgatttcca gcccaccggt tttacccgta ccactttcaa catccacacc
     8161 ctctactgat ctcagagcct ccgccagatc gtggtactgg ctggactgca attcctcctg
     8221 gctaatcaca gaaacactgg cggctgcgtt agtcagcttt ttctcatagc cggatgcaga
     8281 gacaatcatc acatcctctg cagcagctat gctgctggct gaaaatccga gacagggaat
     8341 gactacggaa gccagagtgg ttattcgcat attactaatc tccgatcatg ttaaccggca
     8401 agacaataca taaatacaaa caataatgag aataatttat atttgccttt tacaaaaaat
     8461 aaagattatt gacaaaacta aatataaata acgactgcct cggcatgcca gttaacagct
     8521 atccgtatgg cgtgttcggt tttgcgaata aaaaatggtt actgtagaag cggatttcca
     8581 ccacatgtca accatgaatg gtcagtggtg cgagacgact gggatgattt ggtcgatgac
     8641 ttcttccggc gaatactgat agaaacattg aaatattgta agagaaagcg ttcaaaattc
     8701 tgtggcacgt gaaaaattct acagcgtcat caccttattc agtcgacctt caaaataaac
     8761 gtttaacaga gacaatcacc atttggattg tccgctgagg aggcgaacga tttcctagac
     8821 agctttatca tgtcagccca cattgttggc gctgacaatg gatttgcccc tatatttcca
     8881 gacacctgtt atcacttaac ccatcactgg cccactgccg cagatattcc cgtggcgagc
     8941 gataacccag cgcactatgc ggatgccatt cgttataatg ctcgaacgcc tctgcaaggt
     9001 tctttgctgc cgttaacccg tctggtttgg gcatgatact tatgtagtca cgctttatcg
     9061 ttttcacgaa gctctctgct attccgttac tctccggact ccgcactgcc gtgttcttcg
     9121 gttcaagtcc caacatcctg gcgaactgac gtgtttcatt cgcccggtag catgaaccat
     9181 tatccgtcag ccactccact ggagacgccg gaagctcgct gccaaagcgg cgttccactg
     9241 ctcccagcat gacgtcctgt actgtttcac tgtcgaagcc acccgttgtg accgcccagt
     9301 gcagtgcctc acggtcacag cagtccagcg cgaacgtgac ccgcagtttt tctccgttat
     9361 cacagcggaa ctcaaacccg tcagagcacc atcgctgatt actttctttc acagccactc
     9421 tgccggtatg tgcccgtttc gatggcggta cagcgggttt tcgctcaagc aacagcgcat
     9481 tctggcgcat ggtccggtaa a
//
LOCUS       DAHHTG010000156         9405 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.159, whole genome shotgun sequence.
ACCESSION   DAHHTG010000156 DAHHTG010000000
VERSION     DAHHTG010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9405)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9405)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9405
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.159"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            <1..336
                     /locus_tag="NBI74_003966"
     CDS             <1..336
                     /locus_tag="NBI74_003966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase family protein"
                     /protein_id="HCD1934225.1"
                     /translation="EKTEAVFRLFRPVHPVVAVSARTGWEVDTLVSALMTALPDHAAS
                     PLMTRLQDELRTESVRSQAREQFTGAVDRIFDTAESVCIASVARTVLRAVRDSVVSVA
                     RAVWNWIFF"
     gene            709..3555
                     /gene="ag43"
                     /locus_tag="NBI74_003967"
     CDS             709..3555
                     /gene="ag43"
                     /locus_tag="NBI74_003967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter adhesin Ag43"
                     /protein_id="HCD1934226.1"
                     /translation="MKRHLNTSYRLVWNHITGTLVVASELARSRGKRAGVAVALSLAA
                     VTSVPALAADKVVQAGETVNDGTLTNHDNQIVLGTANGMTISTGLEYGPDNEANTGGQ
                     WIQNGGIANNTTVTGGGLQRVNAGGSVSDTVISAGGGQSLQGQAVNTTLNGGEQWVHE
                     GGIATGTVINEKGWQAIKSGAMATDTVVNTGAEGGPDAENGDTGQFVRGNAVRTTINK
                     NGRQIVAAEGTANTTVVYAGGDQTVHGYALDTTLNGGNQYVHNGGTASGTVVNSDGWQ
                     IIKEGGLADFTTVNQKGKLQVNAGGTATNVTLTQGGALVTSTAATVTGSNRLGNFTVE
                     NGKADGVVLESGGRLDVLEGHSARKTLVDDGGTLAVSVGGKATGVTMTSGGALIADSG
                     ATVEGTNASGKFSIDGISGQASGLLLENGGSFTVNAGGQAGNTTVGHRGTLTLAAGGS
                     LSGRTQLSKGASMVLNGDVVSTGDIVNAGEIRFDNQTTQDAVLIRAVAKGDSPVTFHK
                     LTTSNLTGQGGTINMRVRLDGSNASDQLVINGGQATGKTWLAFTNVGNSNLGVATSGQ
                     GIRVVDAQNGATTEEGAFALSRPLQAGAFNYTLNRDSDEDWYLRSENAYRAEVPLYAS
                     MLTQAMDYDRILADSRSHQTGVNGENNSVRLSIQGGHLGHDNNGGIARGATPESSGSY
                     GFVRLEGDLLRTEVAGMSLTTGVYGAAGHSSVDVKDDDGSRAGTVRDDAGSLGGYLNL
                     VHTSSGLWTDIVAQGTRHSMKASSDNNDFRARGWGWLGSLETGLPFSITDNLMLEPQL
                     QYTWQGLSLDDGQDNAGYVKFGHGSAQHVRAGFRLGSHNDMNFGKGTSSRDTLRDSAK
                     HSVRELPVNWWVQPSVIRTFSSRGDMSMGTAAAGSNMTFSPSRNGTSLDLQAGLEARV
                     RENITLGVQAGYAHSVSGSSAEGYNGQATLNVTF"
     gene            3626..3859
                     /locus_tag="NBI74_003968"
     CDS             3626..3859
                     /locus_tag="NBI74_003968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001117568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 family protein"
                     /protein_id="HCD1934227.1"
                     /translation="MPGCTSRLLPEGPFSRNQALAVTTAYLNVLIEDDQGTHFRLVIR
                     NAEGQLRWRCWNFEPDAGKQLNPYLASEGILRQ"
     gene            3950..4768
                     /locus_tag="NBI74_003969"
     CDS             3950..4768
                     /locus_tag="NBI74_003969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF945 domain-containing protein"
                     /protein_id="HCD1934228.1"
                     /translation="MRLASRFGYVNQIRRERPLTREELMYHVPSIFGEDRHTSRSERY
                     AYIPTITVLENLQREGFQPFFACQTRVRDQSRREYTKHMLRLRRAGQITGQHVPEIIL
                     LNSHDGSSSYQMLPGYFRAICTNGLVCGQSLGEVRVPHRGNVVDSVIEGAYEVVGVFD
                     RIEEKRDAMQSLVLPPPSRQALAQAALTYRYGDEHQPVTTADILTPRRREDYGKDLWS
                     AYQTIQENMLKGGISGRNARGKRIHTRTIHSIDTDIKLNRALWVMAETLLESMR"
     gene            4860..5345
                     /locus_tag="NBI74_003970"
     CDS             4860..5345
                     /locus_tag="NBI74_003970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HCD1934229.1"
                     /translation="MTTVTHNSTTPSVSVTAASGNNPPQLVATLVPDEQRISFWPQHF
                     GLIPQWVTLEPRVFGWMDRLCEDYCGGIWNLYTLNNGGAFMAPEPDDDDDETWVLFNA
                     MNGNRAEMSPEAAGIAACLMTYSHHACRTENYAMTVHYYRLRDYALQHPECSAIMRII
                     D"
     gene            5357..5833
                     /locus_tag="NBI74_003971"
     CDS             5357..5833
                     /locus_tag="NBI74_003971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RadC family protein"
                     /protein_id="HCD1934230.1"
                     /translation="MQQLSFLPGAMTPGERSLIQRALKTLDRHLHEPGVAFTSTRAAR
                     EWLILNMAGLEREEFRVLYLNNQNQLIAGETLFTGTINRTEVHPREVIKRALYHNAAA
                     VVLAHNHPSGEVTPSKADRLITERLVQALVLVDIRVPDHLIVGGSQVFSFAEHGLL"
     gene            5902..6123
                     /locus_tag="NBI74_003972"
     CDS             5902..6123
                     /locus_tag="NBI74_003972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 domain-containing protein"
                     /protein_id="HCD1934231.1"
                     /translation="MKIITRGEAMRIHRQHPASRLFPFCTGKYRWHGSAETYTGREVQ
                     DIPGVLAVFAERRKDSFGPYVRLMSVTLN"
     gene            6197..6565
                     /locus_tag="NBI74_003973"
     CDS             6197..6565
                     /locus_tag="NBI74_003973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system YeeU family
                     antitoxin"
                     /protein_id="HCD1934232.1"
                     /translation="MSDTLSGTTHPDDNDDRLWWGLPCTVTPCFGARLVQKGNRLHYL
                     ADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSGELNPRHQHTVTLYAKGLTCEAD
                     TLGSCGYVYLAVYPTPETKK"
     gene            6655..7032
                     /locus_tag="NBI74_003974"
     CDS             6655..7032
                     /locus_tag="NBI74_003974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001683029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin"
                     /protein_id="HCD1934233.1"
                     /translation="MNTLPDTHVREASRCPSPVTIWQTLLTRLLDQHYGLTLNDTPFA
                     DERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGFSACTRSQLINSIDILRARRATG
                     LMTRNNYRMVNNITQGKHPEAKR"
     gene            7029..7517
                     /locus_tag="NBI74_003975"
     CDS             7029..7517
                     /locus_tag="NBI74_003975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1934234.1"
                     /translation="MKLALTLEADSVNVQALNMGRIVVDVDGVNLSELINKVSENGYL
                     LRVVDKSDQHATSTPPPLTTLTCIRCSTAHITETDNAWLYSLSHQTNDDGESEWIHFT
                     GSGYLLRTDAWSYPVLRLKRLGLSKTFRCLVVTLTRRYGVSLIHLDASAECLPGLPTF
                     NW"
     gene            7529..7750
                     /locus_tag="NBI74_003976"
     CDS             7529..7750
                     /locus_tag="NBI74_003976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1934235.1"
                     /translation="MKSLTTETALDILIAWLQDNIDCESGIIFDNDEDKTDSAALLPC
                     IEQGGYPYPAPTAASATEPVSLTHQTSFH"
     gene            7806..8651
                     /locus_tag="NBI74_003977"
     CDS             7806..8651
                     /locus_tag="NBI74_003977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000065751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
                     /protein_id="HCD1934236.1"
                     /translation="MSNITISHPEVVTGNTDVICSTSVSHILAVRKSTLLQIDILIRQ
                     LAEISAMTESIGGKTALDWAMKQDFRCGCWLMEKPETAMKAITRNLDREIWRDLMQRS
                     GMLSLMDAQARDTWYRSLEYDNFPEISEANILSTFEQLHQNKDEVFERGVINVFRGLS
                     WNYKNNSPCKFGSKIIVNNLVRWDRWGFHLNNGPQADRLADLERMLHLFSGKPIPDNR
                     ENITIHLDEHIRSVQGKECYEDEMFSIRYFKKGSAHITFRKPELVDRLNDIIARHYPE
                     MLPSQ"
BASE COUNT         2304 a         2488 c         2487 g         2126 t
ORIGIN      
        1 gaaaaaacgg aggcggtatt ccgtctgttc cggcctgtac atccggttgt ggccgtatcg
       61 gcccgcaccg gctgggaagt ggatacgctg gtcagtgcgc tcatgacagc gcttcccgac
      121 catgccgcca gtcccctgat gacccgcctg caggacgagc tgcgcacgga atctgtccgg
      181 tctcaggccc gtgaacagtt taccggtgcg gtggaccgga tatttgacac ggcggagagc
      241 gtctgtattg cctctgttgc acgcacggtt ctgcgtgccg tccgtgactc ggtggtctct
      301 gttgcccgcg ctgtatggaa ctggattttc ttctgaacag tctgccaggg gaaatgcctg
      361 attacattgc gttaaccaac tgaaaacatg acacaaacac ctcatgctcc tcctgtttct
      421 cttgcgtgac cgccttgcaa tcaatagaat aaaacgatcg ataaaacagg tatcgatcgt
      481 tactatcgat cgtttatagt ggttaatggt ctgcgaaaag acagctgaat ctcttcatca
      541 tgcagaacgg aattgcacac aacggactga tacttctctg gcggatgaga gaggggaagc
      601 atttatgccc gggaaaaacc actttatctg accatgctgt tttgtacctg ccggtatcca
      661 catttgtggg taccggcttt tttattcacc ctctgtaagg aaaagctgat gaaacgacat
      721 ctgaacacca gctacaggct ggtatggaat cacattacgg gcaccctggt ggtggcctcc
      781 gaactggccc gctcacgggg aaaacgcgct ggtgtggcgg ttgcgctgtc tcttgctgct
      841 gtcacatcag tcccggcact ggctgctgac aaggttgtac aggcgggaga aaccgtgaac
      901 gacggaacac tgacaaatca tgacaaccag attgtcctcg gtacggccaa cggaatgacc
      961 atcagtaccg gtctggagta tgggccggat aacgaggcca ataccggcgg acaatggata
     1021 caaaatggcg gtatcgccaa caacactact gtcaccggtg gtggtcttca gagagtgaat
     1081 gccggaggaa gcgtttcaga cacggttatc agtgccggag gcggacagag ccttcagggg
     1141 caggcagtga acaccactct gaacggcggt gagcagtggg tacatgaagg cgggattgca
     1201 acgggtaccg tcattaatga gaagggctgg caggccatca aatccggcgc aatggcaacc
     1261 gacacggttg tgaataccgg cgcggaaggg ggaccggatg cggaaaatgg tgataccggg
     1321 cagtttgttc gcggaaatgc cgtacgcacc accatcaata aaaatggtcg tcagattgtg
     1381 gctgctgaag gaacggcaaa taccactgtg gtttatgccg gcggcgacca gactgtacat
     1441 ggctacgcgc tggataccac actgaacggc ggtaaccagt atgtgcacaa cggcggtacg
     1501 gcgtctggca ctgttgtaaa cagtgacggc tggcagatta tcaaggaagg tggtctggcg
     1561 gatttcacca ccgttaacca gaaaggcaaa ctgcaggtga acgccggtgg tacagccacg
     1621 aatgtcaccc tgacgcaggg cggcgcactg gtcaccagta cggcggcaac cgtcaccggc
     1681 agcaaccgtc tgggcaattt cacggtggaa aacggtaagg ctgacggcgt tgttctggag
     1741 tccggcggtc gcctggatgt actggagggc cattcagccc ggaaaacact ggtggatgac
     1801 ggcggtaccc tggcagtgtc tgtcggtggt aaggcaacag gtgtcaccat gacatccggt
     1861 ggtgccctga ttgcagacag tggtgccact gttgagggga ccaatgccag cggtaagttc
     1921 agtattgatg gtatatccgg tcaggccagc ggcctgctgc tggaaaatgg cggtagcttt
     1981 acggttaatg ccgggggaca ggctggcaac accactgtcg gacatcgtgg aacactgacg
     2041 ctggctgccg ggggaagtct gagtggcaga acacagctca gtaaaggcgc cagcatggta
     2101 ctgaatggtg atgtggtcag taccggcgat attgttaacg caggggagat tcgctttgat
     2161 aatcagacga cacaggatgc cgtcctgatc cgtgctgttg caaaaggcga ctccccggta
     2221 acgttccata aactgaccac cagtaacctc accggtcagg gtggcaccat caatatgcgt
     2281 gttcgccttg atggcagtaa tgcctctgac cagctggtga ttaatggtgg tcaggcaacc
     2341 ggtaaaacct ggcttgcgtt tacaaatgtc ggaaacagta acctcggggt ggcaacctcc
     2401 ggacagggta tccgggttgt ggatgcacag aatggtgcca ccacagaaga aggtgcgttt
     2461 gccctgagtc gcccgcttca ggccggcgcc tttaactaca ccctgaaccg tgacagcgat
     2521 gaagactggt acctgcgcag tgaaaatgct tatcgtgctg aagtccccct gtatgcctcc
     2581 atgctgacac aggcaatgga ctatgaccgg attctggcag actcccgcag ccatcagacc
     2641 ggtgtaaacg gtgaaaataa cagcgtccgt ctcagcattc agggcggtca tctcggtcac
     2701 gataacaacg gcggtattgc ccgtggggcc acgccggaaa gcagcggcag ctatggcttc
     2761 gtccgtctgg agggtgacct gctcagaaca gaggttgccg gtatgtcact gacgacagga
     2821 gtgtatggtg ctgcaggcca ttcttccgtt gatgttaagg atgatgacgg ctcccgtgcc
     2881 ggcacggtcc gggatgatgc cggcagtctg ggcggatacc tgaatctggt acacacgtcc
     2941 tccggcctgt ggactgacat tgtggcacag ggaacccgcc acagcatgaa agcgtcatcg
     3001 gacaataacg acttccgcgc ccggggctgg ggctggctgg gctcactgga aaccggtctg
     3061 cccttcagta tcactgacaa tctgatgctg gagccacaac tgcagtacac ctggcaggga
     3121 ctctccctgg atgacggcca ggataacgcc ggttatgtga agttcgggca tggcagtgca
     3181 caacatgtgc gtgccggttt ccgtctgggc agccacaacg atatgaactt tggtaaaggc
     3241 acctcatccc gtgacaccct gcgcgacagt gcaaaacaca gtgtgcgtga actgccggtg
     3301 aactggtggg tacagccttc tgttatccgc accttcagct cccggggaga catgagcatg
     3361 ggtacagccg cagccggcag taacatgacg ttctcaccgt cccggaatgg cacgtcactg
     3421 gacctgcagg ccggactgga agcccgtgtc cgggaaaata tcaccctggg cgttcaggcc
     3481 ggttatgccc acagcgtcag cggcagcagc gctgaaggtt ataacggtca ggccacgctg
     3541 aatgtgactt tctgataatc aggtcactcc ccttcaggcc ccggtcatca tgaccggggc
     3601 cttttactta cagggagcaa cagatatgcc gggttgtaca tcacgtttat tgcccgaagg
     3661 ccctttctca cgcaaccagg ctcttgccgt cacaacggct taccttaatg tgcttattga
     3721 agatgaccag ggaacgcatt tccggctggt gatccgcaac gctgaaggcc agttgcgctg
     3781 gcggtgctgg aattttgaac ctgatgccgg aaaacagcta aatccgtatc tcgccagtga
     3841 gggaattctc aggcaataaa cgtcattcac ttgatccatc aggccgcgtc ttctccggaa
     3901 gacgcggctt tttcatttat accgcttaat cattcataag gagccaatta tgcgattagc
     3961 cagccgtttt ggttatgtaa atcagatccg ccgggagcgc ccgctgacac gcgaagaact
     4021 gatgtaccac gtcccgagta tttttggaga agaccggcac acctcccgca gtgaacggta
     4081 tgcgtacatt cccaccatca ccgtcctgga aaatctgcag cgggaaggct ttcagccgtt
     4141 cttcgcctgc cagacccgtg tgcgcgacca gagccgccgg gaatatacca aacatatgct
     4201 gcgtctgcga cgggccggac agataaccgg tcagcatgtg cctgaaatta ttctgctcaa
     4261 ctcccatgac ggttcatcca gctaccagat gttacccgga tattttcgtg ccatctgcac
     4321 caatggcctg gtctgcggtc agtcgctggg agaagtccgg gtgccacacc ggggaaacgt
     4381 ggtggacagt gtgattgaag gtgcttacga ggtggtgggg gtttttgacc ggattgaaga
     4441 aaagcgtgac gccatgcagt cgctggtcct gccgccaccg tcacgccagg cgctggcaca
     4501 ggcggcactg acttaccgtt atggtgacga acatcagccc gtcaccaccg ccgacattct
     4561 gacgccacga cgccgggagg attacggtaa ggacctgtgg agtgcttatc agaccatcca
     4621 ggagaatatg ctgaaaggcg ggatttccgg tcgcaatgcc agaggaaaac gtatccatac
     4681 ccgtaccatt cacagcatcg acaccgacat taagctcaac cgggcgttgt gggtgatggc
     4741 agaaacactg ctggagagca tgcgctgatg ctttttccct gaaagcgcag tcctgactca
     4801 cttccgtccc tgcctccgga cattcttcca ttcatttact ttttataagg aataatctca
     4861 tgacaaccgt tacgcataat tccaccacac cttctgtttc cgtaaccgct gcatcaggga
     4921 ataacccacc tcagctggtt gctactctcg tccctgatga acagcgcatc agcttctggc
     4981 cgcagcattt tggcctcatt ccacagtggg tgaccctgga gccccgtgtc ttcggctgga
     5041 tggaccgtct gtgcgaagac tactgtggtg gtatctggaa tctgtacacc ctgaacaacg
     5101 gcggggcatt tatggcaccc gaaccggatg acgatgatga cgaaacatgg gtactgttca
     5161 atgccatgaa cggtaaccgc gctgaaatga gcccggaagc cgccggtatt gctgcctgtc
     5221 tgatgacgta cagccatcat gcctgtcgta cggagaatta tgccatgacg gtccattatt
     5281 accggttgcg ggattacgcc ctgcagcatc cggaatgcag cgccattatg cgcatcattg
     5341 actgaggaga gaggccatgc aacagctttc ctttctgccc ggagcgatga cgcccggcga
     5401 gcgcagcctt attcagcggg ccctgaaaac cctggaccgt catcttcatg aacccggcgt
     5461 ggccttcacc tccacccgtg cggcacggga atggctgatt ctgaacatgg cgggactgga
     5521 gcgtgaagaa ttccgggtac tgtatctgaa taaccagaat cagctgattg ccggtgaaac
     5581 cctcttcacc ggcaccatca accgcacgga agtccatccc cgggaagtga ttaaacgcgc
     5641 cctgtatcac aatgccgctg ctgtggtgct ggcacacaat cacccgtccg gtgaagtcac
     5701 ccccagtaag gcagaccgcc ttatcacgga acgtctggta caggcactgg tcctggtgga
     5761 tatccgggta ccggaccatc tgatagttgg tggcagtcag gttttctctt ttgcggaaca
     5821 cggtctgctt taacccgtca ccgtcacaac cacatcacac ctgttttcac ttttatcttc
     5881 tgtcttcaga ggtatcacac tatgaaaatc atcacccgtg gtgaagccat gcgtattcac
     5941 cgtcagcatc cggcatcccg tctttttccg ttctgcaccg gtaagtaccg ctggcacggc
     6001 agtgctgaga cgtataccgg tcgtgaagta caggatattc ccggtgtgct ggccgtgttt
     6061 gctgaacgcc gtaaggacag ttttggcccg tatgtccggc tgatgagcgt cactctgaac
     6121 tgagtcagga gaaatgatga gccgaattat cgctgccacc ggaccattct taactgattt
     6181 ctgtgagaac tttatcgtgt cagacacact ttccgggaca acgcatcccg atgacaatga
     6241 cgaccgcctc tggtggggac tgccctgcac cgtgacgccc tgtttcgggg cacgtctggt
     6301 gcagaagggt aaccggttgc attaccttgc cgaccgcgcc ggtatcagag gccggttcag
     6361 cgatgcagat gcataccacc tggaccaggc ctttccgctg ctgatgaaac aactggaact
     6421 catgctcacc agcggtgaac tgaatccccg ccatcagcat accgtgacac tgtatgcgaa
     6481 ggggctgacc tgtgaagccg acaccctcgg ctcctgtggt tacgtttatc tggctgttta
     6541 tccgacacct gaaacgaaaa agtaaaccac cagaataacc ttctgctccg gcctggtgtt
     6601 ttcatcacgc cactgtccca ttttttatct ctgcatatca ggaaaatctt cagtatgaac
     6661 acattacccg acacacacgt acgggaggca tcgcgctgcc cgtctcccgt caccatctgg
     6721 cagacactgc tcacccgact gctggaccag cactacggcc tcacgctgaa tgacacaccg
     6781 ttcgccgatg aacgtgtgat tgagcagcat attgaggcag gcatttcact gtgtgatgcg
     6841 gtgaactttc tcgtggaaaa atacgcgctg gtacgaactg accagccagg attcagcgcc
     6901 tgtacccgct ctcagttaat caacagcatt gatattctcc gggctcgcag ggcgaccggc
     6961 ctgatgaccc ggaacaatta cagaatggta aataacatta cccagggcaa gcatccggag
     7021 gcaaaacgat gaaactggcc ctgacgctgg aagccgacag cgttaacgtg caggcactga
     7081 acatggggcg cattgtcgtt gacgtcgatg gtgttaatct cagtgaactg attaacaagg
     7141 tctctgaaaa cggttattta ctccgcgtgg tcgataaatc cgaccaacac gcaaccagta
     7201 caccaccacc gttaacaacc cttacctgca tacgttgcag caccgcacat atcacggaaa
     7261 cggacaacgc ctggctgtac tcgctgtcac accagactaa tgacgacggc gaatcagaat
     7321 ggattcattt tacaggcagc ggctatctgt tacgtaccga tgcgtggtca tatccggttc
     7381 tgcggcttaa gcgcctgggc ctgtcaaaaa cgttccgttg tctggttgtc acacttaccc
     7441 gacgttatgg cgtcagtctc attcatctgg atgccagcgc tgaatgcctg ccgggtttac
     7501 ccactttcaa ctggtaacca ggaacaacat gaaatcatta accacggaaa cagcactgga
     7561 tattctgatt gcgtggctgc aggacaatat cgactgcgaa tcgggaatta tctttgacaa
     7621 cgatgaggat aaaacggatt cggcagcact gctgccctgc attgaacagg gaggatatcc
     7681 ataccctgcg ccaactgcag cttctgcaac agaaccggtg agccttactc accagacttc
     7741 attccactga cgccagcctg aatacggctg gcgttttcgt ttataaacca aaagggaaac
     7801 cgataatgtc aaacatcaca atctcccatc cggaagtggt caccgggaat acggacgtta
     7861 tctgctcaac ctcagtcagc cacattctgg ctgtacgaaa gagtacactg ttgcaaatcg
     7921 acatacttat ccggcaactg gctgaaatct cagcaatgac agaaagtatt ggcggtaaaa
     7981 ccgcactgga ctgggcgatg aaacaggatt ttcgctgcgg atgctggctc atggagaaac
     8041 ctgaaacagc aatgaaagcc atcacccgca accttgaccg cgaaatctgg cgtgacctga
     8101 tgcaacgttc cgggatgctg tcattaatgg atgcgcaggc ccgtgataca tggtaccggt
     8161 cactggagta cgataatttt ccggaaatca gtgaagcgaa cattctgagc acatttgaac
     8221 aactgcacca gaataaggat gaggtgtttg agcgaggggt aattaatgtt ttcaggggac
     8281 tgagctggaa ttacaaaaac aattcaccct gtaaatttgg cagtaaaatt atcgtcaaca
     8341 atctggtgag gtgggacaga tggggatttc atctgaacaa cggcccacag gccgatcgac
     8401 ttgccgacct ggaaagaatg ctgcatctgt tcagcggcaa accgatcccc gacaaccggg
     8461 aaaacatcac tattcatctg gatgagcaca tccggtctgt tcagggtaaa gagtgctatg
     8521 aagatgaaat gttcagcatc agatacttta agaagggctc cgcgcacatc acgttcagga
     8581 aaccagaact ggttgatcgg ctaaatgata ttatagccag acactatccg gaaatgttac
     8641 cttcacaata gagtactaag aattgattca attatgtatt ttagcttcat tccttcatgc
     8701 aaaatcattt gggggttctt atgggggtta ctcatttatc caacacatac aaattattta
     8761 agatcaatgc attaatactt atattcgact cctgtgatcg ccaaaatgcc tcttcagtga
     8821 tccccatcct gcttatttct gagcaacctg accctgctga tgtatctggt agtatgacag
     8881 agtgattcat ttttcttgcc gagtgtacga aaacgtcttc cttgccaccg accatcaacc
     8941 gtcactcaaa aaaacctctc ttttttcaag ctatcaagaa taagttgcat tttatctgca
     9001 ccggacacca gccatccata tatggctttt acctgctaca aagatgaaga agtgcgtaaa
     9061 tgtgatgatt aaaacaatca aagaaaaatt acaggtgcaa taatttcact ataacattat
     9121 aatccatggt gaaaataaca ctgcacaagg attgtcatca tcaaactcat cggaactatt
     9181 ttttctgtcg tcattggtgt aatactgatt gtaagtatcc tttcaggaga ttatttttgc
     9241 cgtgtggggc atggaacttg taaagcagcc aacagcgcta ggcatttaat tgaaagtaaa
     9301 gagaaaaaca gaagtagtcg cgtcaaagtc tttagcacac atattaattt gagtcatcca
     9361 gcggcatcct ggctagcaac tcaaacttgc gctaacaata tattc
//
LOCUS       DAHHTG010000157         9337 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.160, whole genome shotgun sequence.
ACCESSION   DAHHTG010000157 DAHHTG010000000
VERSION     DAHHTG010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9337
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.160"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            complement(145..2412)
                     /gene="adiA"
                     /locus_tag="NBI74_003978"
     CDS             complement(145..2412)
                     /gene="adiA"
                     /locus_tag="NBI74_003978"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418541.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase"
                     /protein_id="HCD1934237.1"
                     /translation="MKVLIVESEFLHQDTWVGNAVERLADALSQQNVTVIKSTSFDDG
                     FAILSSNEAIDCLMFSYQMEHPDEHQNVRQLIGKLHERQQNVPVFLLGDREKALAAMD
                     RDLLELVDEFAWILEDTADFIAGRAVAAMTRYRQQLLPPLFSALMKYSDIHEYSWAAP
                     GHQGGVGFTKTPAGRFYHDYYGENLFRTDMGIERTSLGSLLDHTGAFGESEKYAARVF
                     GADRSWSVVVGTSGSNRTIMQACMTDNDVVVVDRNCHKSIEQGLMLTGAKPVYMVPSR
                     NRYGIIGPIYPQEMQPETLQKKINESPLTKDKVGQKPSYCVVTNCTYDGVCYNAKEAQ
                     DLLEKTSDRLHFDEAWYGYARFNPIYADHYAMRGEPGDHNGPTVFATHSTHKLLNALS
                     QASYIHVREGRGAINFSRFNQAYMMHATTSPLYAICASNDVAVSMMDGNSGLSLTQEV
                     IDEAVDFRQAMARLYKEFTADGSWFFKPWNKEVVTDPQTGKTYDFADAPTKLLTTVQD
                     CWVMHPGESWHGFKDIPDNWSMLDPIKVSILAPGMGEDGELEETGVPAALVTAWLGRH
                     GIVPTRTTDFQIMFLFSMGVTRGKWGTLVNTLCSFKRHYDANTPLAQVMPELVEQYPD
                     TYANMGIHDLGDTMFAWLKENNPGARLNEAYSGLPVAEITPREAYNAIVDNNVELVSI
                     ENLPGRIAANSVIPYPPGIPMLLSGENFGDKNSPQVSYLRSLQSWDHHFPGFEHETEG
                     TEIIDGIYHVMCVKA"
     gene            complement(2611..3519)
                     /gene="melR"
                     /locus_tag="NBI74_003979"
     CDS             complement(2611..3519)
                     /gene="melR"
                     /locus_tag="NBI74_003979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator MelR"
                     /protein_id="HCD1934238.1"
                     /translation="MNTDTFMCSSDEKQTRSPLSLYSEYQRMEIEFRAPHIMPTSHWH
                     GQVEVNVPFDGDVEYLINNEKVNINQGHITLFWACTPHQLTDTGTCQSMAIFNLPMHL
                     FLSWPLDKDLINHVTHGMVIKSLATQQLSPFEVRRWQQELNSPNEQIRQLAIDEIGLM
                     LKRFSLSGWEPILVNKTSRTHKNSVSRHAQFYVSQMLGFIAENYDQALTINDVAEHVK
                     LNANYAMGIFQRVMQLTMKQYITAMRINHVRALLSDTDKSILDIALTAGFRSSSRFYS
                     TFGKYVGMSPQQYRKLSQQRRQTFPG"
     gene            3802..5157
                     /gene="melA"
                     /locus_tag="NBI74_003980"
     CDS             3802..5157
                     /gene="melA"
                     /locus_tag="NBI74_003980"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HCD1934239.1"
                     /translation="MMSAPKITFIGAGSTIFVKNILGDVFHREALKTAHIALMDIDPT
                     RLEESHIVVRKLMDSAGASGKITCHTQQKEALQDADFVVVAFQIGGYEPCTVTDFEVC
                     KRHGLEQTIADTLGPGGIMRALRTIPHLWQICEDMTEVCPDATMLNYVNPMAMNTWAM
                     YARYPHIKQVGLCHSVQGTAEELARDLNIDPATLRYRCAGINHMAFYLELERKTADGS
                     YVNLYPELLAAYEAGQAPKPNIHGNTRCQNIVRYEMFKKLGYFVTESSEHFAEYTPWF
                     IKPGREDLIERYKVPLDEYPKRCVEQLANWHKELEEYKNASRIDIKPSREYASTIMNA
                     IWTGEPSVIYGNVRNDGLIDNLPQGCCVEVACLVDANGIQPTKAGTLPSHLAALMQTN
                     INVQTLLTEAILTENRDRVYHAAMMDPHTAAVLGIEEIYALVDDLIAAHGDWLPGWLH
                     R"
     gene            complement(5154..5804)
                     /locus_tag="NBI74_003981"
     CDS             complement(5154..5804)
                     /locus_tag="NBI74_003981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000198764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2238 domain-containing protein"
                     /protein_id="HCD1934240.1"
                     /translation="MTRTLKPLILNTGALALTLILIYTGISAHDKLTWLMEVTPVIIV
                     VPLLLATAKRYPLTPLLYTLIFFHAIILMVGGQYTYAKVPVGFEVQEWLGLSRNPYDK
                     LGHFFQGLVPALVAREILVRGMYVRGRKMVAFLVCCVALAISAMYELIEWWAALAMGQ
                     GADDFLGTQGDQWDTQSDMFCALLGALTTVIFLARFHCRQLRRFGLITRAPEAITS"
     gene            complement(5927..7573)
                     /gene="fumB"
                     /locus_tag="NBI74_003982"
     CDS             complement(5927..7573)
                     /gene="fumB"
                     /locus_tag="NBI74_003982"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HCD1934241.1"
                     /translation="MSNKPFIYQAPFPMGKDNTEYYLLTSDYVSVADFDGETILKVEP
                     EALTLLAQQAFHDASFMLRPAHQKQVAAILHDPEASENDKYVALQFLRNSEIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGGDEEALSKGVYNTYIEDNLRYSQNAPLDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCVAKGGGSANKTYLYQETKALLTPGKLKNFLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAHYYDELPTEGNEHGQAFRDIQLEQEL
                     LEEAQKLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINREGIW
                     IEKLEHNPGQYIPEELRQAGEGEAVKVDLNRPMKEILAQLSQYPVSTRLSLTGTIIVG
                     RDIAHAKLKELIDAGKELPQYIKDHPIYYAGPAKTPAGYPSGSLGPTTAGRMDSYVDL
                     LQSHGGSMIMLAKGNRSQQVTDACHKHGGFYLGSIGGPAAVLAQQSIKHLECVAYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIVNKQCANCTK"
     gene            complement(7651..8991)
                     /gene="dcuB"
                     /locus_tag="NBI74_003983"
     CDS             complement(7651..8991)
                     /gene="dcuB"
                     /locus_tag="NBI74_003983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuB"
                     /protein_id="HCD1934242.1"
                     /translation="MLFTIQLIIILICLFYGARKGGIALGLLGGIGLVILVFVFHLQP
                     GKPPVDVMLVIIAVVAASATLQASGGLDVMLQIAEKLLRRNPKYVSIVAPFVTCTLTI
                     LCGTGHVVYTILPIIYDVAIKNNIRPERPMAASSIGAQMGIIASPVSVAVVSLVAMLG
                     NVTFDGRHLEFLDLLAITIPSTLIGILAIGIFSWFRGKDLDKDEEFQKFISVPENREY
                     VYGDTATLLDKKLPKSNWLAMWIFLGAIAVVALLGADSDLRPSFGGKPLSMVLVIQMF
                     MLLTGALIIILTKTNPASISKNEVFRSGMIAIVAVYGIAWMAETMFGAHMSEIQGVLG
                     EMVKEYPWAYAIVLLLVSKFVNSQAAALAAIVPVALAIGVDPAYIVASAPACYGYYIL
                     PTYPSDLAAIQFDRSGTTHIGRFVINHSFILPGLIGVSVSCVFGWIFAAMYGFL"
BASE COUNT         2210 a         2301 c         2504 g         2322 t
ORIGIN      
        1 aaaaaaaagc gcggggtggg accgcgcaaa aagcatgaca ttttttgttt gcagcaattt
       61 ttatgaaaac gttgccatac ttaaaaggcc tcaagccagg cggcgtaatg ttatttaaca
      121 attacgcctt cagcggaata gtggttacgc tttcacacac ataacgtggt aaataccgtc
      181 aataatttct gtcccttctg tttcgtgttc aaatccaggg aaatgatggt cccaggattg
      241 cagcgagcgt aaataactta cttgcggact gtttttatcg ccgaagtttt caccagacag
      301 cagcatcggg attcctggcg gatacgggat aactgagttt gccgcgatac gtcctggcag
      361 attttcaatg gataccagtt cgacattgtt gtcgacaatc gcgttgtacg cttcacgcgg
      421 ggtgatttcc gccaccggta ggccggaata ggcttcattc aaccgtgcgc cagggttgtt
      481 ttctttcagc caggcaaaca tggtgtcacc cagatcgtga atccccatgt tcgcgtaagt
      541 gtcaggatat tgttcaacaa gttccggcat cacctgcgcc agcggtgtgt tggcatcata
      601 gtggcgtttg aaggagcaaa gggtgttaac cagagtcccc catttcccac gggtcacgcc
      661 catagagaac aggaacataa tctggaagtc agtggtacgg gtaggcacaa tgccgtggcg
      721 accaagccag gctgtgacca gcgccgccgg aacaccggtt tcttccagtt caccatcttc
      781 acccattccc ggagcaagga tgctgacttt aatcgggtcg agcatactcc agttatccgg
      841 aatatctttg aagccgtgcc agctttcgcc cggatgcatg acccagcagt cctgaacggt
      901 ggtcagcagt ttggttggtg cgtcagcaaa gtcataggtt ttgccggttt gtgggtcggt
      961 gacgacttct ttgttccacg gtttgaagaa ccagctaccg tcagcggtga actctttata
     1021 tagccgcgcc atcgcctgac ggaaatcaac cgcttcgtcg atcacttcct gggtcagtga
     1081 caggccgctg ttgccgtcca tcatcgacac cgccacgtcg ttggatgcgc agatggcata
     1141 cagcggggag gtggtggcat gcatcatgta ggcctggttg aagcgggaga agttaatcgc
     1201 cccacgacct tcacgtacat gaatataaga ggcctgtgac agcgcattca gcagtttgtg
     1261 ggtggagtgg gtggcgaaaa cggtaggacc gttgtgatcg ccaggttcgc cgcgcatggc
     1321 atagtgatcg gcatagatcg ggttgaaacg tgcatagccg taccaggctt cgtcaaagtg
     1381 cagacgatcg gaggtttttt ccagcagatc ctgcgcttct ttagcgttat aacacacgcc
     1441 gtcataggta cagttggtca ccacgcagta agacggtttt tgcccgactt tgtctttagt
     1501 cagcgggctt tcattgattt tcttctgcaa ggtttcaggt tgcatttcct gcggatagat
     1561 tggcccgata atgccgtagc ggttgcggct tggcaccatg tagaccggtt tcgcacctgt
     1621 cagcatcaag ccttgttcga tggatttatg gcagttacgg tcaacgacca cgacatcgtt
     1681 atcggtcatg caagcctgca tgatggtgcg gttagagccg gaagtaccga cgactaccga
     1741 ccaggagcga tcggcaccaa atacgcgtgc ggcatatttt tcgctttcgc caaatgcgcc
     1801 agtatggtca agcaaagaac cgagggaagt gcgttcgatg cccatgtcgg tgcggaacag
     1861 attttcacca tagtagtcat ggtagaaacg tccggcgggt gttttagtaa aaccaacgcc
     1921 gccctggtgg cctggcgctg cccaggaata ttcatggatg tcgctatatt tcatcagcgc
     1981 gctgaacagt ggcggtaata gctgctggcg gtagcgggtc atcgcggcaa cggcgcgtcc
     2041 ggcgataaag tcggcggtat cttccagaat ccaggcgaat tcatcgacaa gctccagcag
     2101 gtcgcgatcc attgcggcga gggctttttc ccgatcgccc aacaggaaga ccggcacgtt
     2161 ttgttggcgc tcatgaagct taccgatcaa ttgtctgacg ttttgatgtt cgtccggatg
     2221 ttccatttga tagctgaaca tcaggcagtc aatggcttcg tttgaagaga gaatggcaaa
     2281 accatcatca aaggaggtgg atttaatcac ggtaacattt tgctggctta aagcatctgc
     2341 cagacgctca acggcgttac cgacccaggt gtcttgatgg agaaactcgc tttcaacaat
     2401 taatactttc atcattgctt acccggttat gaaggaatct tcgttgcggg caagtatctt
     2461 ccggcctttt cgggctgtaa agcgcgtgaa aaacaaacat ttttgaaata tttattttta
     2521 taaacaatat gttatgtgat tattttaaat tattttcact gcttattatc gacggctaaa
     2581 ctattttttt ggctgatact gatatcgtct ttagccggga aacgtctggc ggcgctgttg
     2641 gctaagtttg cggtattgtt gcggcgacat gccgacatat ttgccgaacg tgctgtaaaa
     2701 acggctactc gaacgaaagc ctgccgtcag ggcaatatcg agaatacttt tatcggtatc
     2761 gctcagtaac gcgcgaacgt ggttgatgcg catcgcggtt atgtactgtt tcatcgtcaa
     2821 ttgcatgacc cgctggaata tccccattgc atagttggcg ttaagtttga cgtgctcagc
     2881 cacatcgttg atggtcagcg cctgatcata gttttcggca ataaagccca gcatctggct
     2941 gacataaaat tgcgcatggc gcgagacgct gtttttgtgt gtgcgcgagg ttttattgac
     3001 cagaatcggt tcccagccag agaggctaaa tcgcttgagc atcaggccaa tttcatcaat
     3061 ggcgagctgg cgaatttgct cgttcggact gtttaattcc tgctgccagc ggcgcacttc
     3121 aaacgggcta agttgctgtg tcgccagtga tttgatcacc atgccgtgag taacgtggtt
     3181 aatcaggtct ttatccagcg gccaggagag aaacagatgc atcggcagat taaaaatcgc
     3241 catgctctga caggttccgg tatctgttag ttggtgcggt gtacaggccc agaacagcgt
     3301 gatatgaccc tgattgatat tcactttttc attgttgatc aggtattcca catcgccatc
     3361 gaaaggcaca ttcacttcga cctgaccatg ccagtggctg gtgggcatga tatgcggtgc
     3421 gcgaaactca atttccatcc gctggtattc tgaatacagc gacagtgggc tacgggtctg
     3481 cttttcgtcg ctgctgcaca taaacgtatc tgtattcatg gatgactctc tttcctggaa
     3541 tatcagaatt atggcaggag tgagggagga tgactgcgaa taggcgcacg gttttattcc
     3601 tctccctgga ggtgcgagga gagcggatgt gaagattggg gtcgattatt cagcttttca
     3661 ggtaatttat tcccataaac tcagatctat tgatgtgcca cttaggatat gagtttatgg
     3721 gaatgctcaa cctggaagcc ggaggttttc tgcagattcg cctgccatga tgaagtcatt
     3781 caagcaagcc aggagatctg catgatgtct gcacctaaaa ttacatttat cggcgctggt
     3841 tcgacgattt tcgttaaaaa tattcttggt gatgtgttcc atcgtgaggc gctgaaaacg
     3901 gcgcatattg ccctgatgga tatcgatccc acccgcctgg aagagtcaca cattgtggtg
     3961 cgcaagttga tggattcagc aggggccagc ggcaaaatca cctgccacac ccaacagaaa
     4021 gaagccttac aggatgctga ttttgtggtg gtggcatttc agattggcgg ttatgaacct
     4081 tgcacggtga ctgatttcga ggtctgtaag cggcatggtc tggaacaaac cattgccgat
     4141 acgctggggc cgggcggcat tatgcgtgcg ctacgtacca ttccgcacct gtggcagatt
     4201 tgcgaggaca tgacggaagt ctgccctgat gccaccatgc tcaactacgt taacccaatg
     4261 gcgatgaata cctgggcgat gtatgcacgc tatccgcata tcaagcaggt agggctgtgc
     4321 cattcggtgc agggaacggc ggaagagctg gcgcgtgacc tcaatatcga tccggctacg
     4381 ctgcgttacc gttgcgcagg tatcaaccat atggcgtttt acctggaact ggagcgtaaa
     4441 accgcagacg gtagctacgt gaatctctat cctgaattgc tggcggctta tgaagcaggg
     4501 caggcaccga agccaaatat tcatggtaat actcgctgcc agaatattgt gcgttacgaa
     4561 atgttcaaaa agctgggcta cttcgtcaca gaatcgtcag aacattttgc tgagtacaca
     4621 ccgtggttta ttaagccagg tcgtgaggat ttgattgagc gttataaagt accgctggat
     4681 gagtacccga aacgctgcgt cgagcagctg gcgaactggc ataaagagct ggaggagtat
     4741 aaaaacgcct cccggattga tattaaaccg tcacgggaat atgccagcac aatcatgaac
     4801 gctatctgga ctggcgagcc gagtgtgatt tatggcaatg tccgtaacga tggtttgatt
     4861 gataacctgc cacaaggatg ttgcgtggaa gtggcctgtc tggttgatgc taatggcatt
     4921 cagccgacca aagccggtac gctaccttcg catctggccg ccctgatgca aaccaacatc
     4981 aacgtacaga cgctgctgac agaagctatt cttacggaaa atcgcgaccg tgtttaccac
     5041 gccgcgatga tggacccgca tactgccgcc gtgctgggca ttgaagaaat atatgctctt
     5101 gttgacgacc tgattgccgc ccacggcgac tggctgccag gctggttgca ccgctaagag
     5161 gttatcgctt ccggcgctct ggttatcaag ccaaagcgcc gtagctggcg gcaatgaaag
     5221 cgagcgagga atatcaccgt cgttaatgca ccaagcagcg cgcagaacat atcggattgc
     5281 gtgtcccatt ggtcgccctg ggtgccgaga aaatcatccg ctccctggcc cattgctagt
     5341 gccgcccacc actcgattaa ttcatacatc gcgcttatcg ccagcgccac gcagcagacc
     5401 agaaacgcca ccatcttacg tccgcgaacg tacatcccgc gtacaaggat ctctcgtgcc
     5461 accagtgcag gcaccagccc ctggaaaaaa tgccccagtt tgtcatacgg attacggctc
     5521 aaccccagcc attcctgcac ctcaaaacca acggggactt tcgcgtaggt gtattgcccg
     5581 ccgaccatca ggatgatggc gtgaaagaaa atgagcgtat agagcagcgg cgttaacgga
     5641 taacgtttag cggtggcaag cagtagcggc acgacaataa tcaccggtgt cacttccatc
     5701 agccaggtga gtttgtcatg ggccgaaatg ccggtataaa tcaggattaa cgttagcgcc
     5761 agtgcgccgg tgttaagaat taacggcttg agtgtgcggg tcatggtgag ttcgcaagtc
     5821 agggaaaata ctgactattg accagtaagc gtgaaattac aatcgcaaaa gaggtggccc
     5881 agggggatca cctggcagca tgctgccagg cgctgggccg aagaggttac ttagtgcagt
     5941 tcgcgcactg tttgttgacg atctgctgga agaagtcgtt acctttgtca tcgaccagga
     6001 taaacgccgg gaaatcttct acttcgattt tccagatagc ttccataccc agctccggat
     6061 aagcgacgca ttccagatgc ttgatgctct gctgcgccag taccgccgcc ggaccgccga
     6121 tgctaccgag gtagaagccg ccgtgtttat gacacgcgtc ggtaacctgc tgactgcggt
     6181 tgcctttcgc cagcatgatc atgctgccgc cgtgggattg cagcagatcc acgtaggagt
     6241 ccatacgacc tgcggtggtt gggccaagtg aacctgatgg ataaccggca ggcgttttcg
     6301 ccggacccgc gtagtagatc gggtgatctt tgatgtactg cggcagttct ttaccggcgt
     6361 caatcagctc tttcagcttg gcgtgtgcaa tatcgcgacc cacgataatg gtgccggtga
     6421 gcgacaagcg ggtggatacc gggtattgcg aaagctgggc aaggatctct ttcatcgggc
     6481 ggttaaggtc gactttcact gcttcgcctt cacctgcctg acgcagttct tctggaatgt
     6541 actggcctgg gttgtgttcc agtttttcga tccagatacc ttcgcggttg attttcgctt
     6601 taatgttacg gtcagcggag caggagactc ccatgcctac cgggcaggat gcgccgtgac
     6661 gtggcagacg gataacgcgg atgtcgtgcg cgaagtattt accgccaaac tgcgcgccaa
     6721 gaccgagttt ctgggcctct tccagcagtt cctgttccag ttggatatcg cggaacgcct
     6781 gaccatgttc gttcccttcc gtcggcagtt catcgtaata gtgagcgctt gctaacttga
     6841 cggttttcag gttggtttcc gcagacgtac cgccaatcac aaacgcgata tggtacggcg
     6901 ggcaggctgc agtaccgagg gtacgcattt tctcgacgag gaagtttttc agtttgccgg
     6961 gcgtcagcag ggctttggtt tcctggtaga gatacgtttt gttggcagag ccgccgcctt
     7021 tagcgacaca gaggaatttg tactcatcgc catctaccgc gtacaggtcg atttgcgcag
     7081 gcaggttcgt gccggtgttg acctctttat acatgtccag cggcgcgttc tgtgaatagc
     7141 gcaggttatc ttcgatatag gtgttataga cgcctttcga cagcgcttct tcatcaccgc
     7201 caccggtcca cacgcgttga ccttttttac cgacgatgat cgcggtgccg gtatcctggc
     7261 aggtcggcag cacgcctttg gcggcgattt cggagtttct taagaattgc agcgccacgt
     7321 acttgtcgtt ttcgctggct tctggatcgt gaagaatagc cgcaacctgt ttctggtgtg
     7381 ccgggcggag catgaaagaa gcgtcgtgaa aggcttgctg cgccagcagg gtcagggctt
     7441 ctggttccac tttcaggatg gtttcgccgt cgaagtcggc aacgctaacg taatcggaag
     7501 tgagtagata gtattcggta ttgtctttcc ccatcgggaa aggtgcctgg tagataaagg
     7561 gtttgtttga catagcttcc agcctgtaac tctgtatttg ttattcgaaa atggcgtgcc
     7621 gcgtagtcac gggcggcacg caaagtgcat ttataagaac ccgtacatcg cggcgaagat
     7681 ccagccgaag acgcacgata cgctcacacc aatcaacccc ggcagaataa agctgtggtt
     7741 gatgacgaag cgaccgatgt gggtggtgcc ggaacggtca aactgaatcg ctgccagatc
     7801 gctcggataa gtcggcagga tgtaataacc gtagcaagcc ggtgctgaag ccacgatgta
     7861 tgccggatca acgccgatcg ccagcgcgac cggaacaatc gccgccagcg ccgcagcctg
     7921 agagtttaca aacttggaaa ccagcagcag aacaatggca taggcccacg gatactcttt
     7981 caccatttca cccagtacgc cctgaatttc agacatatgc gcaccgaaca tggtttcagc
     8041 catccatgcg ataccgtaca ccgccacgat ggcgatcata ccggaacgga agacttcgtt
     8101 ttttgagata gacgcgggat tggttttggt caggataata atcagcgccc cggtcagcag
     8161 cataaacatc tgaataacca gtaccatcga cagcggtttg ccgccgaagg atggacgcag
     8221 gtccgaatca gcaccaagaa gggcgactac agcgattgcc ccgaggaaga tccacattgc
     8281 cagccagttg cttttcggca gttttttatc cagcagcgtc gcggtatcac cgtaaacata
     8341 ctcacggttt tccggtacgg agatgaattt ctggaactct tcgtctttat ccagatcttt
     8401 accgcggaac cagctgaaga taccgatcgc caggataccg attaacgtcg atggaatggt
     8461 gattgccagc aggtcgagga actcaagatg gcgaccatca aaggtgacat tacccagcat
     8521 cgcaaccaga gatacgaccg caaccgacac cggactggcg ataatcccca tctgtgcacc
     8581 gatagaactt gccgccatcg gacgttccgg acggatgttg ttcttaatag caacgtcgta
     8641 gatgatcggc agaatggtgt aaaccacatg acccgtaccg caaagaatgg tcagcgtaca
     8701 ggtcacaaac ggcgcgacaa ttgagacata tttcgggttg cgacgcagca gcttctcggc
     8761 aatttgcagc atgacatcaa gaccgcccga agcttgcaag gtcgccgatg ccgccaccac
     8821 cgcaatgata accagcatga catcaaccgg tggtttacct ggctgaaggt ggaagacgaa
     8881 gaccagaatg accagaccga taccgcctaa taaacccagc gcgataccac cctttctggc
     8941 accataaaac agacatatca gtattatgat aagttggata gtaaataaca tgtgtgaacc
     9001 ctcgcgataa tcctatttaa atttttgctg aatagatcac agtcacgttc tgttttgtat
     9061 gaactgtttt cagagcctga ctaaatatcc gtttggtcgt taccggcttg agcaaatacc
     9121 tcacagtgaa tattggctga ttaaagcagc ggcaaattta tgcactgtga taagcggctt
     9181 tttcagagag aaagactctc tggcgatggg tttatagttt gtacagcgca gatgttaatg
     9241 ttttgtttgc tgttatctta ttgatattta tcggtctgtt ttttgttctc agggcaggta
     9301 ttattaggtt tatagattaa tctgatctac ccatttg
//
LOCUS       DAHHTG010000158         9311 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.161, whole genome shotgun sequence.
ACCESSION   DAHHTG010000158 DAHHTG010000000
VERSION     DAHHTG010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9311)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9311)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9311
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.161"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            151..828
                     /locus_tag="NBI74_003984"
     CDS             151..828
                     /locus_tag="NBI74_003984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence hypothetical
                     protein"
                     /protein_id="HCD1934243.1"
                     /translation="MSIIFNGHYRMKHRTWITEALRLHFEEHLPRVVAGRRLGVPKST
                     VCSMFVRFRRAGLSWPLPAGMSEQELDACLYGQFSTVPVVRPESTVISEAPVVKKRPR
                     RPNFPYEFKIALVEQSLQPGACVAQIARENGINDNLLFNWRHQYRKGGLLPSGKNMPA
                     LLPVTLTPEPDNKIPAPAQEPEQINTPSDSLCCELVLPAGTLRLKGKLTPALLQTLIR
                     EIKGSSH"
     gene            828..1175
                     /gene="tnpB"
                     /locus_tag="NBI74_003985"
     CDS             828..1175
                     /gene="tnpB"
                     /locus_tag="NBI74_003985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HCD1934244.1"
                     /translation="MISLPAGSRIWLVAGITDMRNGFNGLASKVQNVLKDDPFSGHLF
                     IFRGRRGDQIKVLWADSDGLCLFTKRLERGRFVWPVTRDGKVHLTPAQLSMLLEGINW
                     KHPKRTERAGIRI"
     gene            1195..2766
                     /locus_tag="NBI74_003986"
     CDS             1195..2766
                     /locus_tag="NBI74_003986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HCD1934245.1"
                     /translation="MDTSLAHENARLRALLQTQQDTIRQMAEYNRLLSQRVAAYASEI
                     NRLKALVAKLQRMQFGKSSEKLRAKTERQIQEAQERISALQEEMAETLGEQYDPVLPS
                     ALRQSSARKPLPASLPRETRVIRPEEECCPACGGELSSLGCDVSEQLELISSAFKVIE
                     TQRPKQACCRCDHIVQAPVPSKPIARSYAGAGLLAHVVTGKYADHLPLYRQSEIYRRQ
                     GVELSRATLGRWTGAVAELLEPLYDVLRQYVLMPGKVHADDIPVPVQEPGSGKTRTAR
                     LWVYVRDDRNAGSQMPPAVWFAYSPDRKGIHPQNHLAGYSGVLQADAYGGYRALYESG
                     RITEAACMAHARRKIHDVHARAPTYITTEALQRIGELYAIEAEVRGCSAEQRLAARKA
                     RAAPLMQSLYDWIQQQMKTLSRHSDTAKAFAYLLKQWDALNVYCSNGWVEIDNNIAEN
                     ALRGVAVGRKNWMFAGSDSGGEHAAVLYSLIGTCRLNNVEPEKWLRYVIEHIQDWPAN
                     RVRDLLPWKVDLSSQ"
     gene            3181..3408
                     /gene="daaF"
                     /locus_tag="NBI74_003987"
     CDS             3181..3408
                     /gene="daaF"
                     /locus_tag="NBI74_003987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F1845 fimbrial adhesin operon transcriptional
                     regulator DaaF"
                     /protein_id="HCD1934246.1"
                     /translation="MKINKLTLNERKNDILSYFGEIDAPFRTSEVAEHLGVSAYQARH
                     YLQCLEREGKIKRSPVRRGASTLWEISSIPP"
     gene            complement(3671..4612)
                     /locus_tag="NBI74_003988"
     CDS             complement(3671..4612)
                     /locus_tag="NBI74_003988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HCD1934247.1"
                     /translation="MSRKKEKNTSTPHDAAFRAFMASPEVARDFVSLHLPEEYRQLCD
                     LSTLRLEPCTFVDQELKQYACDALFSMKTVSGEDGYVYCLVEHQSTENAYMAFRMLRY
                     AVAAMQRHFEAHKVLPLVIPVLFYHGERSPYPYSMNWLDGFSDPDIAAKIYTRAFHLV
                     DVTVMDDDEIMHHRRLGALTLLMKHSRSRDLMLQMDRLVQLIQDSLNEELAQVLFHYL
                     LNGSEHVTVTFLQTLAQRLPQHEDNIMTLAERIEQKGIAKGIERGLAQGIEQGKLEGK
                     LEGKLETACNMLNAGMDRQTVLQMTGLSNDQLNMLKH"
     gene            complement(4672..7584)
                     /locus_tag="NBI74_003989"
     CDS             complement(4672..7584)
                     /locus_tag="NBI74_003989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1934248.1"
                     /translation="MLRGPFLRRISAFFTRQELTAMKICLYHTLNPEAIPGYKKFAQA
                     IEADNFVQADVRKIDTNLYRARLSIRDRLLFSLYRYQGETVCLVLEYIRNHAYHTSRF
                     LRKNVVIDEDRLQQQPVPDPVDTVTEALTYINPSHGRFHRLDKMLSFDDDQQALYEHP
                     LPLVIIGSAGSGKTALVLEKMKQAAGDILYLSLSSFLVEKARTLYNASGEGSEVQNID
                     FLSLTEFLETLRIPEGREVTFRAFSDWLPRNRTTAALGSAHTLYEEFRGVIGAAASGN
                     GPLSREAYLSLGVRQLLYSMDDRQTVYTLFERYTAWLKQSRQYDSNLLSHQYLPLADP
                     RYDAIFVDEVQDMTPVQLQLVLKTLRHPGQFLLCGDASQIVHPSFFSWSSLKSLFFRR
                     HQGNEAAINILQANYRNGHHVTALANRLLRLRQARFSSIDRESHHFVRSCGQTEGVVR
                     LLEDREETKQELNGKTSRSNRVAVIVMHPEQKAQARCWFSTPLVFSVQEVKGLEYETV
                     ILHNMVSGARQAFDDICEGISPADLEVEARYSRPRDRQDRSAEIYKFFINALYVALTR
                     ATHNVYLVEQQLTHPLWELLALTHQEEQLELKEEISSRDEWQKTAHLLEKQGKQEQAD
                     SIRSRILQTSEVPWQVITAEDARQWKEQILAGTAGKATQLQALEYSLIYSLFPLYNAL
                     YREDFKPAGQPRAKSLQLLEQKYFRTYQVNNPVALLRDIERYGVDHRSPFNLTPLMSA
                     ARAGNIALVQQLLDRGADPSLTGNDGLAAYHYVLSAAVSTPRYAQQKSAQLYTLLKPE
                     SLSLQVDGRLIKLDNRQMAMFLVLLMQALFHTHLGGALVFSEAFSAARLAECVAHLPE
                     ALLPGRRKRRSYISSQLSQHEVNSKHPYGKKLFLRLNHGQYILNPGLKIRQGERWCAV
                     YALQSPEDLGHDLQTYLQDMSPELVKILGGKKDVYKYSEEAVGYWAEMIRRAAQKT"
     gene            complement(7615..8148)
                     /locus_tag="NBI74_003990"
     CDS             complement(7615..8148)
                     /locus_tag="NBI74_003990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001576414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1934249.1"
                     /translation="MQITEALLAEPGDIRRFIQQAVDYWPRLLAVHFTLHSTEGINGQ
                     QIHAFCTSFYRQVHERITERNHMASPSSPVVLRWLREDSEKETMRCLLLFSQGLICHS
                     RASVTVDEECSQVVDLLQQTWRVISAGGQCRLERCFQVARDDTSDQYVALKTAAILLI
                     LPVISTTIRGCVIGDDN"
     gene            complement(9057..>9311)
                     /locus_tag="NBI74_003991"
     CDS             complement(9057..>9311)
                     /locus_tag="NBI74_003991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001522299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1934250.1"
                     /translation="DLHALSDQQKSDCVVLNPEKSHPYIADMAMFLSPLPIVLEWHCV
                     MCDKFFPGKKFCEACGTGIYCRAVCTKSVYSYRSDLLLDP"
BASE COUNT         2277 a         2573 c         2356 g         2105 t
ORIGIN      
        1 caggacgcaa ataaaacact gcaggatatg acatatcccg atgagtcatt aaaaataata
       61 agatgaacac agcacaaatc attaaatctt ctgaatagta accggctcat ttaaaccgtc
      121 tggtctgttt cctccggctc tacaaaaata atgtccatca tttttaatgg acactatcgt
      181 atgaaacacc ggacctggat cactgaagct ttacgtcttc actttgaaga acatttaccc
      241 cgggttgtgg ccgggcgtcg cctgggtgta ccaaaatcaa cagtttgtag tatgttcgtg
      301 cgctttcgga gagctggcct ttcgtggcct ttgcccgcag gcatgtcgga gcaggaactt
      361 gatgcctgcc tttacggaca attttccacg gtaccagtcg tacgtcctga aagcaccgtt
      421 atatccgaag cccccgtggt aaaaaaacgt ccccggcggc ccaacttccc ttatgagttt
      481 aaaatcgcct tagtggagca gtcactgcag cccggagcct gtgtggcgca gatcgcccgg
      541 gaaaacggaa tcaacgataa cctgctcttc aactggcgcc atcaataccg gaaaggtggc
      601 ctgctgcctt ccggaaaaaa tatgccggca ctgcttcccg tgacgttaac gccggagccg
      661 gataataaaa tcccggcccc cgcacaggaa ccagagcaga taaatacacc gtccgacagt
      721 ctgtgttgtg agctggttct gccggccgga actctcaggc ttaaaggtaa actgacgccg
      781 gcgttattac agacacttat ccgcgaaata aaagggagca gccactgatg atatctctcc
      841 ctgcaggttc gcgtatctgg ctggttgcag gtatcaccga tatgcgaaat ggctttaacg
      901 gcctggcatc aaaagttcag aacgtcctga aggatgaccc gttctccgga cacctgttca
      961 tcttccgcgg acgccggggt gaccagataa aagtgttgtg ggctgacagt gacggactgt
     1021 gcctcttcac caaacgcctg gagcggggcc gcttcgtctg gccagtcacc cgtgacggca
     1081 aggtgcacct tactccggct cagttatcca tgcttcttga aggtatcaac tggaagcacc
     1141 cgaaacgaac ggaacgcgct ggaatccgca tataacccgt tgtaaagtga ggatatggac
     1201 acctcacttg ctcatgagaa cgcccgcctg cgggcactgt tgcagacgca acaggacacc
     1261 atccgccaga tggctgaata caaccgcctg ctctcacagc gggtggcggc ttatgcttcc
     1321 gaaatcaacc ggctgaaggc gctggttgcg aaactgcaac gtatgcagtt cggtaaaagc
     1381 tcagaaaaac ttcgtgcaaa aaccgaacgg cagatacagg aagcacagga gcgaatcagc
     1441 gcacttcagg aagaaatggc ggaaacgctg ggtgagcaat atgacccggt actgccatcc
     1501 gccctgcgcc agtcttcagc ccgtaaaccg ttaccggcct cacttccccg tgaaacccgg
     1561 gttatccggc cggaagagga atgctgtcct gcctgtggtg gtgaactcag ttctctggga
     1621 tgtgatgtgt cagagcaact ggagcttatc agcagcgcct ttaaggttat cgaaacacaa
     1681 cgtccgaaac aggcctgttg ccggtgcgac catatcgtgc aggcaccagt accttcaaaa
     1741 cccattgcac gcagttatgc cggagcgggg cttctggccc atgttgtcac cgggaaatat
     1801 gcagaccatc tgccgttata ccgccagtca gaaatatacc gtcgtcaggg agtggagctg
     1861 agccgtgcca cactggggcg ctggacaggt gctgttgctg aactgctgga gccgctgtat
     1921 gacgtcctgc gccagtatgt gctgatgccc ggtaaagtcc atgctgatga tatccccgtc
     1981 ccggtccagg agccgggcag cggtaaaacc cggacagccc ggctgtgggt ctacgtccgt
     2041 gatgaccgta acgccggttc acagatgccc ccggcggtct ggttcgcgta cagtccggac
     2101 cggaaaggta tccatccaca aaatcacctg gccggttaca gcggtgtgct tcaggccgat
     2161 gcttacggtg gttaccgggc gttatacgaa tccggcagaa taacggaagc cgcgtgtatg
     2221 gctcatgccc ggagaaaaat ccacgatgtg catgcaagag cgcccaccta catcaccacg
     2281 gaagccctgc agcgtatcgg tgaactgtat gccatcgagg cagaggtccg gggctgttca
     2341 gcagaacagc gtctggcggc aagaaaagcc agagccgcgc cactgatgca gtcactgtat
     2401 gactggatac agcaacagat gaaaacactg tcgcgtcact cagatacggc aaaagcgttc
     2461 gcatacctgc tgaaacagtg ggatgcactg aacgtgtact gcagtaatgg ctgggtggaa
     2521 atcgacaaca acatcgcaga gaacgcctta cggggagtgg ccgtaggccg gaaaaactgg
     2581 atgttcgcgg gttccgacag cggtggtgaa catgcggcgg tgttgtactc gctgatcggc
     2641 acatgccgtc tgaacaatgt ggagccagaa aagtggctgc gttacgtcat tgaacatatc
     2701 caggactggc cggcaaaccg ggtacgcgat ctgttgccct ggaaagttga tctgagctct
     2761 cagtaaatat caatacggtt ctgacgagtc gcttactctg aatatacggg acacagaaag
     2821 ggaaatccgg tacatcacct gttttcgtat tgtatgaata cccgtggcgc gacagcggac
     2881 tttattactg caaaccgtca tgcttcccgg cctcagagga gagtcacatc agcatgagcg
     2941 accaggacca caccggtgag tgtttttaag atgaaaggta tggtggcagt cacagttgcg
     3001 ggaaccggca tacacccgtg acgataaaca actgtgcaga atgacaacag gagaaatgaa
     3061 cagtaatgag gggcatcatt cgcagaacgc cggatatcat acatcatccc cggtcagctg
     3121 gacgcatcac cgccagagga actataatca tccggaggct gaccatctgt taataacggc
     3181 atgaaaataa acaagttaac actgaatgaa cgtaagaatg acattctttc ttatttcgga
     3241 gaaatcgatg cccccttcag aacctcagaa gttgctgaac atttaggggt aagtgcttat
     3301 caggccagac attacctgca gtgtctggag agggaaggga aaattaaacg ctcaccggta
     3361 cgacggggag ccagtacgct gtgggaaatc agcagtattc cgccataacc cgtcagaaca
     3421 gatgtcgttg cggacaaaaa gcggttccgg aaaacatttt accactgtga atgaccatca
     3481 tccctgttca gacagcggct gtttccgtga aaaaaatcaa ctggttgctg gcgatataat
     3541 gataatacct tcacggagtg ggtgagggac agggctcacc cgtccagtgc aataaaatat
     3601 gtgagaatga cacaatataa ataaccgcct ccgggtgcga atgtcccgga agcgctccgg
     3661 actaatattg tcagtgtttc agcatattca gctggtcatt tgaaagcccc gtcatctgca
     3721 gcaccgtctg acggtccatt ccggcattca gcatattgca ggcagtttcc agcttccctt
     3781 ccagcttccc ttccagttta ccctgctcaa tcccctgtgc cagtcctcgc tcaatgccct
     3841 tggcaatacc tttctgctca atgcgttcag ccagtgtcat aatgttgtcc tcgtgctggg
     3901 gcagacgctg cgccagcgtc tgcaaaaacg tcaccgtcac atgttcactg ccattcagca
     3961 gatagtgaaa cagtacctgt gccagttctt cattcaggga gtcctgtatc agctgaacca
     4021 gcctgtccat ctgtaacata aggtcacgac tgcggctgtg cttcatcagc agcgtcaggg
     4081 cccccagacg gcggtgatgc ataatttcat cgtcatccat caccgtcaca tccaccagat
     4141 ggaacgctct ggtgtagatt ttagccgcaa tatccgggtc agaaaagcca tccagccagt
     4201 tcatgctgta cggatacgga ctgcgctcac cgtggtagaa cagcaccggt atcaccagcg
     4261 gaagtacctt atgcgcctca aagtgtcgct gcatcgcagc taccgcatac ctgagcattc
     4321 tgaacgccat atacgcgttt tccgtactct ggtgctccac gaggcagtaa acatagccat
     4381 cctcaccact gacagtcttc atactgaaca gtgcatcaca tgcatactgc ttcagctcct
     4441 ggtccacaaa cgtgcagggc tccagccgca gcgtcgataa atcacacaac tgacggtatt
     4501 cctccggaag atgcagcgag acaaaatccc ttgccacctc cggcgacgcc ataaatgccc
     4561 tgaaagcagc atcgtgcggc gtggatgtgt tcttctcttt tttcctgctc accctgtctc
     4621 ccttatgaag gtgttatgac atattctcct gcattctgtc aggactgaac gtcacgtttt
     4681 ctgagcggca cgacggatca tctccgccca gtatccgaca gcctcttcac tgtatttata
     4741 aacatccttt ttccctccca ggattttcac taattccgga gacatatcct gcagataagt
     4801 ctgcaaatca tgacccagat cttccggtga ctgcaacgca taaaccgcac accacctttc
     4861 cccctgcctg attttaagtc ccgggttaag aatatactgt ccgtggttca gcctcagaaa
     4921 gagctttttg ccatacgggt gtttgctgtt aacttcatgc tgagaaagct ggctgctgat
     4981 ataactcctt cgtttgcgac gtcccggcag cagcgcctcc ggaagatggg ccacacactc
     5041 tgccagtcgt gccgcactga aggcttctga aaaaaccaga gcacctccca gatgcgtgtg
     5101 gaacagcgcc tgcatcagga gcaccaggaa catggccatc tgccggttgt ccagtttgat
     5161 gagtcgtccg tccacctgca gtgagagact ttctggtttc agcagggtat acaactgcgc
     5221 gcttttctgt tgcgcataac ggggcgtact gaccgccgct gacagtacat aatgataagc
     5281 cgccagcccg tcattacccg tcagcgaggg atctgcgcca cgatcaagca gttgctgaac
     5341 cagggcgata ttacctgccc gggctgctga catcagcgga gtaagattaa acggcgagcg
     5401 gtgatcaaca ccataacgct caatatcccg caacagcgca accgggttat tcacctggta
     5461 tgtccggaaa tacttctgct ccagcaactg cagactcttc gccctgggct gccctgcggg
     5521 cttaaaatct tcccggtaca gcgcattata cagcgggaaa agggaataaa tcagactgta
     5581 ctccagcgcc tgcaattgtg tggctttacc tgccgtgcct gcgaggatct gttctttcca
     5641 ctgccgggcg tcctctgcgg tgatgacctg ccatggcacc tcagacgtct gcaggatccg
     5701 gctgcggatg ctgtccgcct gctcctgctt cccctgtttc tcaagcagat gtgcggtttt
     5761 ctgccattca tcacggctgg aaatctcttc cttcagctcc agttgctctt cctgatgcgt
     5821 gagcgcaagc agctcccata acgggtgcgt cagttgctgt tcaaccagat acacgttgtg
     5881 tgtggcacgg gtgagcgcaa catagagcgc gttgataaaa aacttataga tttccgctga
     5941 acgatcctgc ctgtcccgcg gacgggaata acgggcctca acctcaaggt ctgccggaga
     6001 aatgccctca cagatatcat caaacgcctg ccgggcgccg ctgaccatgt tgtggaggat
     6061 caccgtttcg tattccagtc ccttcacttc ctgaactgaa aacaccagcg gtgtactgaa
     6121 ccagcaccgt gcctgtgctt tctgttccgg atgcatcaca ataaccgcca cccgattcga
     6181 acgtgatgtt ttgccattca gttcctgttt tgtctcttcc ctgtcttcca gtaaccggac
     6241 aacgccttcc gtctgaccac agcttctgac gaaatggtga ctttcccgat caatagaact
     6301 aaagcgggcc tgtctgagtc tcagcagccg gttagccagt gcagtgacgt ggtgcccgtt
     6361 acggtagttc gcctgcagaa tattaatggc agcctcgttc ccctggtgtc gccggaaaaa
     6421 gagacttttg agcgaactcc atgagaaaaa gctggggtgt acaatctgac tggcatcacc
     6481 gcagagtaaa aactgaccgg gatgacgtaa cgttttcagt accagctgca gctgaacagg
     6541 cgtcatatcc tgaacttcat caacgaaaat ggcatcgtaa cggggatctg ccagcggcag
     6601 gtactgatgg ctgagcagat tactgtcata ctgacgcgac tgtttcagcc atgccgtata
     6661 acgttcaaac agggtataga ccgtctggcg gtcatccata ctgtagagca actgccgtac
     6721 ccccagcgac agatacgcct cccgggacag cggtccgttg ccggacgcag ccgcaccaat
     6781 cacaccccgg aactcttcat acagggtatg ggcagatccc agtgcagccg tggtcctgtt
     6841 acggggcagc cagtcgctga acgcacggaa ggtcacttcc cggccttccg gaatgcgcag
     6901 ggtttccaga aactccgtca gcgacagaaa gtcaatattc tgcacctcgc tcccttcacc
     6961 cgacgcgtta taaagcgttc gcgctttttc caccagaaat gaggacaggc tgaggtacag
     7021 tatatcacca gcagcctgct tcattttttc cagtaccagc gctgtcttgc cactccccgc
     7081 agagccgata atcaccagcg gcagcggatg ttcgtacagt gcctgctgat catcatcaaa
     7141 ggacaacatt ttatccagac ggtgaaaacg cccgtgagac ggattgatgt aggtcagcgc
     7201 ttcagttacc gtatcaacag gatccggcac aggttgttgt tgtaagcggt cctcatcaat
     7261 gaccacgttt ttgcgaagaa aacgtgaagt gtgataggca tgattgcgaa tatattccag
     7321 tacaaggcag actgtttccc cctgatagcg atacagtgaa aacagtaacc ggtcccggat
     7381 actgagccgt gcgcggtaaa gattagtgtc aattttacga acatcagcct gaacgaagtt
     7441 atcggcctca atggcctgtg caaatttttt atagcccggg atcgcttccg ggttcagggt
     7501 atggtagaga cagattttca tggctgtcag ttcctgtcgt gtaaaaaacg cactgattct
     7561 acgaaggaac ggccctcgca acaaccttgc tcccttcctg aatgaaattc acgttcagtt
     7621 atcgtcaccg atcacacagc ccctgattgt tgtggatata accggtaata tcaacaatat
     7681 cgcagctgtt tttaacgcaa cgtactggtc tgatgtatca tcacgggcaa cctgaaaaca
     7741 cctttccagc cggcattgtc cgcctgcgct tatcacccgc caggtctgtt gcagtaaatc
     7801 caccacttgc gaacattctt catcaactgt gacactggcc cgcgaatggc aaatgagtcc
     7861 ctgactgaat aacagcaaac atcgcatcgt ttctttttct gaatcttccc gcaaccagcg
     7921 taacaccacc ggcgaggacg gactggccat gtgattacgc tcagtaatac gttcatgcac
     7981 ctgtcggtaa aaggaagtac agaatgcatg aatctgttgc ccgttgattc cttctgtaga
     8041 atgaagcgtg aagtgaactg ccagcagacg cggccagtag tctacggcct gctgaataaa
     8101 acgccggatg tctcccggct ctgcaagtaa ggcttcggtt atctgcacga aatgttcctt
     8161 ttcaaaccgt tgcattttgt taagccggtg cagacacacg ggaaagcacg cctgcaccgg
     8221 tgtaatgcca gttataaaca atgtgaatgg aatgacgaat cagtcaggaa gggatggcaa
     8281 cctgctggct gaccggagaa tgtctgcagg aaggttcacg ggaatgtcgc cagcaccagg
     8341 gacagggccc ggctccacaa taccagcgcc ggcattttgc acactgccag cgcaccggca
     8401 tcacaacacg tttcatgcaa ctccccctcg tgattcctct gttcgctgct gcatccactg
     8461 ctccacttcg cttctgtacc cgcggctgct acgacccggt tttatgggtt caggaaaatg
     8521 gccttcactg atcagcctgt aaaacccttt gtcggatcgg tgaagctggt gatgcctggc
     8581 acatcggcaa gcagcaatac gctgttgtgg cctgttattg aggtcagaat atcctctgtg
     8641 agattgatga aggtataagg gggaactgct gaacggtgct ttcagagctg gtctgctacg
     8701 ggagaccagg gattaccggc aaggtaacgg gcctccggaa cgcgcacacc acgacgatcg
     8761 caggcgaaat cactgaacac ccgtatcact tttgagtctc tcttcgcact ccggtgaacg
     8821 actgtacggc accacatccc gggaggagcg gcttctggta cggtgctgct tcacccggtg
     8881 cgctttcgca tcccggcaca ctgaatcctc cgggattctg gtaagcagaa ctgcagatat
     8941 ccgctgtcgc gtcgttgcac ggtgtgggtg cgaaacaccg gataacatgc aggctggtga
     9001 tacctctgaa cgtcctgagc agcgcggact ttgtcggatt acgtggaaac accacgtcag
     9061 gggtccagca gcaaatctga ccggtagctg taaacagatt tcgtacatac cgctctgcaa
     9121 taaatccccg taccacaggc ctcacagaac ttcttcccgg gaaaaaactt gtcgcacatc
     9181 acacaatgcc actccagcac gatcggtaat ggcgataaaa acatcgccat atccgcaatg
     9241 taagggtggg acttttccgg attcagcacc acacagtccg acttctgttg gtcgctcagg
     9301 gcatgtaaat c
//
LOCUS       DAHHTG010000159         9241 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.162, whole genome shotgun sequence.
ACCESSION   DAHHTG010000159 DAHHTG010000000
VERSION     DAHHTG010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9241)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9241)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9241
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.162"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            complement(<1..1233)
                     /locus_tag="NBI74_003992"
     CDS             complement(<1..1233)
                     /locus_tag="NBI74_003992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000876991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCD1934251.1"
                     /translation="MKYPTGVENHGGKLRIWFVYKGVRVRENLGVPDTAKNRRVAGEL
                     RASVCYAIKTGVFDYAKQFPSSRNLEKFGEARQDLTIKELAEKFLALKETEVAKTSLN
                     TYRAVIKNILSIIGEKNLASSINKEKLLEVRKELLTGYQIPKSNYIVTQPGRSAVTVN
                     NYMTNLNAVFQFGVDNGYLPDNPFKGISPLKESRTIPDPLSREEFIRLIDACRNQQAK
                     NLWCVSVYTGVRPGELCALGWEDIDLKNGTMMIRRNLAKDRFTVPKTQAGTNRVIHLI
                     KPAIDALRSQMTLTRLSKEHIIDVHLREYGRTEKQKCTFVFQPEVSARVKNYGDHFTV
                     DSIRQMWDAAIKRAGLRHRKSYQSRHTYACWSLTAGANPAFIANQMGHADAQMVFQVY
                     GKWMSENNNAQVALLNTQL"
     gene            complement(1268..1519)
                     /locus_tag="NBI74_003993"
     CDS             complement(1268..1519)
                     /locus_tag="NBI74_003993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000005552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase family protein"
                     /protein_id="HCD1934252.1"
                     /translation="MSEVIMIVSPGKWVSEEQLIALKGIKKGTLKKAREKSFMEGREY
                     KHVAHDGMPWDNSPCFYNLEEIDRWIERQASARPRRHLT"
     gene            complement(1592..4033)
                     /locus_tag="NBI74_003994"
     CDS             complement(1592..4033)
                     /locus_tag="NBI74_003994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exoribonuclease"
                     /protein_id="HCD1934253.1"
                     /translation="MSTDKEEFALYCEAKNDKVRKRLGIKGGFYWTTAKKLSVAISRC
                     ITAMDDNDYDEDDFKKPVRVNLPVVDDLPPEGVFDTEFCNRYEKGGKDGITMTFIGPS
                     PSVQDKSASTDNTNINGEDMTEIEESMLLPVSGQELPIRWLAQHGSEKPVTHISRDEL
                     QALHIARAEELPAVTALAISHKTSLLDSLEIRDLHKLVRDTDKVFPNPGNSDLGLITA
                     FFEAYLDADYTDRGLLTKEWMKGNRVSRITRTASGANAGGGNKTDRNPNLVHTLDTLD
                     VEIAAATLPMDFNIYEIPGSVYRRAKEVVLKKESPFKEWSAALRATPGILDYSRAAIF
                     ALIRSVHPEFYHYPGRLQGYINAYLTETDHENPSKETLTAARHTPEKDILEEINREVV
                     TERETEEEKPQPSDAMAGEQATTETMEPDTTEHCQNAQSLDAQSQVSSANQVKVTADE
                     VNKIMQAANISQPDADKLLAVSRGEFVEGISDPNDPKWVKGIQTRDSVNQNQQETEQN
                     DQKAEQNSPNTQQNEPETKQPEPVVQQEPEKICTACGHSGGGNCPDCGAVMGDATYQE
                     IFDGENQPEVQENDPEEMEGTAHQHKENTGGNQHHASDSETGEASDPLIKANGHHNLT
                     STSRAGIHLMIDLETMGKNPDAPIISIGAIFFDPQTGDMGPEFSKTIDLETAGGVIDR
                     DTIKWWLKQSREAQSAIMTDEIPLDDALLQLREFIDENSGEFFVHVWGNGANFDNTIL
                     RRSYERQGIPCPWRYYNDRDVRTIVELGKAIDFDARTAIPFEGERHNALDDARYQAKY
                     VSAIWQKLIPSQADS"
     gene            complement(4127..4318)
                     /locus_tag="NBI74_003995"
     CDS             complement(4127..4318)
                     /locus_tag="NBI74_003995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001070259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1482 family protein"
                     /protein_id="HCD1934254.1"
                     /translation="MNPVFALILTVFLVSGEPVDIAVSVHRTMQECMAAATEQKIPGN
                     CYPVDKVIHQDNNEIPAGF"
     gene            complement(4315..4503)
                     /gene="dicB"
                     /locus_tag="NBI74_003996"
                     /gene_synonym="ftsT"
     CDS             complement(4315..4503)
                     /gene="dicB"
                     /locus_tag="NBI74_003996"
                     /gene_synonym="ftsT"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibition protein DicB"
                     /protein_id="HCD1934255.1"
                     /translation="MKTLLPNVNTSEGCFEIGVTISNPVFTEEAINKRKHERELLNKV
                     CIVSMLARLRLMPKGCAQ"
     gene            complement(5071..5289)
                     /locus_tag="NBI74_003997"
     CDS             complement(5071..5289)
                     /locus_tag="NBI74_003997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1934256.1"
                     /translation="MQKREPVIIAPDYTDDELYEWMRQKINAAQDLKWANEARAKQAE
                     NLSALEQDITNLEKAAALSIARMITYPR"
     gene            complement(5449..5604)
                     /locus_tag="NBI74_003998"
     CDS             complement(5449..5604)
                     /locus_tag="NBI74_003998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000379593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 family protein"
                     /protein_id="HCD1934257.1"
                     /translation="MDTIDLGNNESLVYGVFPNQDGTFTAMTYTKSKTFKTESGARRW
                     LERNSGG"
     gene            complement(5902..6321)
                     /locus_tag="NBI74_003999"
     CDS             complement(5902..6321)
                     /locus_tag="NBI74_003999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309968.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCD1934258.1"
                     /translation="MVHEDKARKEFASRLALACENAGYEQHGRQAEIARRMKLTPKAV
                     SKWFNGETIPRREKLRELATLIGTTPTYLLGEDTEESGQVRFYQELNPRQKIIIDLLD
                     ELPDSETDELLKTLEEKKQKYNAIYEELARKKKQKAS"
     gene            6401..6655
                     /locus_tag="NBI74_004000"
     CDS             6401..6655
                     /locus_tag="NBI74_004000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HCD1934259.1"
                     /translation="MNQKTLEDVIKTVRVAVVADVCGVSQRAIYKWMDNGKLPRTEYT
                     GETNYAEKIALASNGLFSADAILTIGRNKTTTKKLMGVDS"
     gene            6652..7077
                     /locus_tag="NBI74_004001"
     CDS             6652..7077
                     /locus_tag="NBI74_004001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HCD1934260.1"
                     /translation="MKIKHEHIRMAMNAWAHPDGEKVPAAKITKAYFELGMTFPELYD
                     DNHPEALARNTQKIFRWVEKDTPDAVKKIQALLPAIEKAMPPLLVARMRSHSSAYFRE
                     LVETRERLVRDADDFVAVAIAGFNQMNRGGPAGNIVAVH"
     gene            7149..8219
                     /locus_tag="NBI74_004002"
     CDS             7149..8219
                     /locus_tag="NBI74_004002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001736248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage replisome organizer"
                     /protein_id="HCD1934261.1"
                     /translation="MANAWLRLWHDMPNDPKWRTIARVSGQPIATVMAVYIHLLVSAS
                     RNVTRGHIDVTTEDLASALDVTEEVIDSILQTMQGRVLDGDLITGWEKRQVLKEDNGN
                     ISQTAKSPAERKRAQRERERKREQNGDCHGASRNVTHMSRRVTTDKDTDKDTDQEDQN
                     TMVHGVKNATNQAGDVQTVNPGQPAGTTPEADSAYALKADSGAVQQVMTARPEQSHQL
                     QQPEADSAIQREADRVVPENTGQSVGRVDYPDVFEQVWREYPLRAGANPKKSAFSAWK
                     ARLREGVPPEAMLDGVRRYARYLAATGKTGTEFVQRATTFFGPDRNFENPWLLPVSGT
                     NNQRCVNHISEPDNEIPPGFRG"
     gene            8260..8682
                     /locus_tag="NBI74_004003"
     CDS             8260..8682
                     /locus_tag="NBI74_004003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HCD1934262.1"
                     /translation="MAKVFTQEEREKIKGQVVELVRQSGRETLRQLETKTGATRYLMN
                     VLARELVASGDVYNSGYGLFPSEQARKDWQNARKKLSRAKLKKPSAVDPDLIWSLPDG
                     EIRRYDRRHNMICTECRKSEVMQRILSFYQGDVRYLLK"
     gene            8973..>9241
                     /locus_tag="NBI74_004004"
     CDS             8973..>9241
                     /locus_tag="NBI74_004004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001625141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1934263.1"
                     /translation="MGGETKNIDEIAGIISSRIFDELGWKTQNTTDISWDCCLRSHLN
                     AKQLLSENPKKTHPTDVVFKYKDPYSDITQYIQTDLKSYCASTLDG"
BASE COUNT         2311 a         2108 c         2189 g         2633 t
ORIGIN      
        1 taactgtgta tttaacaaag ctacctgtgc attattgttt tcagacatcc attttccgta
       61 tacctgaaat accatttgcg catctgcatg gcccatctgg tttgctataa atgccgggtt
      121 agcaccagct gtcagcgacc agcaggcata agtatgtctc gactgatatg attttcgatg
      181 gcggagtccg gcacgtttta tcgctgcgtc ccacatctgc cttattgagt caacggtaaa
      241 atggtcacca taatttttta ctctcgctga cacttcaggt tgaaaaacaa aggtgcattt
      301 ttgtttttct gttctgccat actctctgag gtgaacatca atgatatgct ctttgctcag
      361 tctcgttaat gtcatctgac tccggagagc gtcgattgct ggcttaataa gatgaatgac
      421 ccgattggtt cccgcctgtg tttttggtac cgtgaaacgg tcttttgcta aatttctcct
      481 gatcatcatt gttccatttt tcagatctat gtcctcccat ccaagtgcac acagctcacc
      541 agggcgaacg ccagtataaa cagaaacaca ccataaattt tttgcttgct gatttctgca
      601 cgcatcgata agacggataa attcctcccg cgaaagagga tccggaatgg ttcttgattc
      661 ctttaatggc gagatcccct taaacggatt atctggcagg taaccgttat caacaccaaa
      721 ctggaacacg gcgttaagat ttgtcatgta attatttaca gttacagccg atctccctgg
      781 ttgtgtaaca atatagttac ttttggggat ctggtatcca gtcagtagct ctttacgaac
      841 ctccagtaat ttttctttat taatcgatga ggcaagattt ttttcaccga ttatgctcag
      901 gatatttttg atgacggcac ggtatgtgtt gagtgatgtt ttggcgactt cagtttcttt
      961 cagtgccaga aatttttcag ccagttcttt tatggttaaa tcttgtcggg cctcaccaaa
     1021 tttttccaga ttgcgtgagg agggaaactg ttttgcatag tcgaaaacac cagtttttat
     1081 tgcgtaacaa acagaggcgc gtagctcacc tgcaacgcgc ctgttttttg ctgtgtcagg
     1141 aacccccagg ttttccctga ctcttacgcc tttataaaca aaccagatac gtaatttccc
     1201 tccatggttt tccacgcctg tcggatattt catttcaact tctctcatta gttagtgtgg
     1261 cttttagtca agtaagatga cgtcttggtc ttgctgatgc ctggcgctca atccagcgat
     1321 caatttcttc caggttgtaa aagcatggac tgttatccca tggcataccg tcatgagcga
     1381 catgcttata ttcccttcct tccataaacg atttttcccg ggcctttttt aacgtacctt
     1441 tttttattcc tttcagcgca attaactgct cttcggatac ccatttgccg ggagagacaa
     1501 tcatgattac ttcgctcatc gatttcttta tctcttacat cagacgagcg ccggttgcag
     1561 aataccagtc acaaccggcg acagttgaac attaagaatc agcctgactc gggatcagtt
     1621 tttgccagat agctgaaacg tattttgcct ggtaacgggc gtcatcaagt gcattatggc
     1681 gctcaccttc gaatggaata gccgttctgg catcgaagtc tatggctttc cccagctcaa
     1741 cgattgtgcg tacatcgcga tcgttgtagt aacgccacgg gcaggggatc ccctgccgtt
     1801 cgtatgagcg gcgcaaaatc gtgttgtcga agttggctcc atttccccag acatgaacaa
     1861 aaaattcacc ggagttttcg tcgataaatt cccgcaattg taacagtgca tcatctaacg
     1921 ggatttcatc ggtcataatg gcagattgcg cttcgcgtga ttgtttaagc caccatttaa
     1981 tggtgtcccg atcaatgact ccgccagcag tttccagatc gatagtctta ctaaattccg
     2041 gtcccatatc tccggtttgc ggatcgaaaa atattgcacc tattgagata atcggggcat
     2101 cgggattttt tcccatggtt tcaaggtcga tcatcagatg aatccccgct ctgctggtgg
     2161 atgtgagatt atgatgaccg ttcgccttaa ttaagggatc tgacgcctcg ccagtttcac
     2221 tatcgctggc atgatgctga ttgccgccag tgttctcctt gtgctgatgc gcagtgcctt
     2281 ccatttcctc cggatcattt tcctgaactt caggctgatt ctctccatcg aatatttcct
     2341 ggtatgttgc gtcacccatc accgcaccac aatcagggca gttgccgcca ccgctatgac
     2401 cgcaggcggt gcagatcttt tccggttcct gttgcactac tggttcaggt tgtttcgttt
     2461 ctggctcgtt ttgttgcgta tttgggctgt tttgttccgc tttctggtcg ttctgttccg
     2521 tttcttgctg gttctggttc acagaatcgc gagtctggat ccccttgacc catttcggat
     2581 cattagggtc gctaatcccc tcaacaaatt caccacgcga tacagcaagt aacttatcgg
     2641 cgtcaggctg gctgatattg gctgcctgca taattttgtt tacttcgtca gcggtgactt
     2701 ttacttggtt agcggaactc acctgcgact gagcatccag cgactgcgcg ttctggcaat
     2761 gttcagttgt atccggttcc attgtttcag ttgttgcctg ttcacctgcc attgcgtcag
     2821 atggttgtgg tttttcttct tctgtttcac gctcagtaac cacctcgcgg ttaatttctt
     2881 ccaggatatc tttttccggc gtatgccggg cagctgtgag agtttccttg ctggggttct
     2941 cgtgatcagt ttccgtcaaa taggcgttga tatacccctg aaggcgtccc gggtagtgat
     3001 aaaattcagg gtgtacgctt cggataagtg caaaaatagc ggcgcgggaa tagtccagaa
     3061 tacccggggt tgcacgaagt gctgcggacc attctttgaa cggactttct tttttcagga
     3121 ctacttcttt tgcgcgacga taaacgctgc ccggaatttc ataaatatta aaatccatcg
     3181 gaagtgtggc tgctgcaatc tccacatcca gtgtgtcgag ggtgtgtact aaattcggat
     3241 tgcgatcggt tttgttccca ccgccagcat tagcaccgga agccgtgcgg gtgatgcgtg
     3301 aaacacgatt tcctttcatc cactcttttg tcagcagacc ccgatcagtg tagtcagcgt
     3361 ccaggtatgc ttcgaaaaaa gcagttatta gtcccaggtc tgaattacca ggattaggga
     3421 aaactttgtc agtgtcacga accagtttgt ggaggtcgcg aatctccagc gagtcgagca
     3481 gactggtttt atgcgaaata gccagggcag taacagccgg tagttcttca gcccgtgcaa
     3541 tgtgtaatgc ctggagttcg tcgcgtgaaa tgtgcgttac tggtttttcg ctgccgtgtt
     3601 gagcaagcca acgaatgggc agttcctgac cggagacagg tagaagcatg ctctcctcaa
     3661 tctcagtcat gtcttcgccg ttgatgttgg tattatcagt gctggctgat ttgtcctgaa
     3721 cagaggggga agggccgata aatgtcattg tgatgccatc tttcccgcct ttttcatagc
     3781 ggttgcagaa ttcagtatca aacacgcctt ctggcggaag gtcgtcaaca acgggcaaat
     3841 tgacgcggac gggtttttta aagtcgtctt catcataatc gttgtcatcc attgcggtaa
     3901 tgcagcggga gattgcaaca gataattttt ttgctgtagt ccagtaaaaa ccacctttaa
     3961 ttcccaggcg ttttcttact ttgtcatttt ttgcttcgca atatagtgca aattcttctt
     4021 tatcagtgct cattattgat aaacctcatc acagatttaa gggtgaacaa atctctgcca
     4081 ttgctgacat ataagaatga aactggatat ttattacggt gctgttttaa aatcctgccg
     4141 ggatttcgtt attatcctgg tgaataactt tatcgaccgg ataacagttg cctggaattt
     4201 tctgttcggt tgctgctgcc atacattcct gcattgttct gtgaacactg actgcaatat
     4261 caactggctc tccggaaaca agaaaaaccg tcagaataag tgcaaatact ggattcattg
     4321 tgcacatcct tttggcatca gacgtaaacg ggccagcatt gaaacaatgc atactttatt
     4381 taataactcc cgttcgtgtt ttcttttgtt aatggcctct tcagtaaata caggattact
     4441 gatagtgaca ccaatttcaa aacaaccttc agacgtatta acgtttggta ataacgtttt
     4501 cattatcgcg ccctcaacaa tgagttttgt gatgcggtgc ctggtgcctc caggtgacgt
     4561 taaccagtta acaattaacg ccggatacag agaatccacc cataacactg tttttggttt
     4621 taactgttcc gcgtgcgctt agccgcattc accgcatcac aaaattcact ttaaaaacgg
     4681 cggcagagca gtcacggagt aaaactgata ccgccaaacg tcaccagaaa attgataaca
     4741 gagggcgttg cagcggggtt gtcacttaag cgtatggtca acctgacaac tcggtgtcct
     4801 caacggggaa ggaataaccc cgccatactt accgccgcgc catttcgcgg gttgccacaa
     4861 ccggaagcgc acggtcgacg aaaatttaac gacaggctat ctatgaacca gctacctcgc
     4921 cgtgcgcttt cgcgttatgg tctgactttt cagggaaata tcctttcagt aaactgtcag
     4981 tgccggatgc tcacccgtgt ccggcgcacg cactccacct cacccgtgga gaactcctta
     5041 attactaacc ttagctttgt tgattagcta ctaacgcggg tatgtaatca ttctggcaat
     5101 gcttaatgcc gctgcttttt ccagattggt gatatcctgc tccagagcgg acagattttc
     5161 agcctgctta gccctggctt cattagccca tttcagatcc tgcgctgcat taattttctg
     5221 gcgcatccac tcataaagtt catcatcggt atagtctggc gcgatgatga cgggttctcg
     5281 tttctgcacg ctgattcctc gcggtgctgt ttcgcttatc agccgttaga ttttgccgaa
     5341 ctggaaagcg cctgtttaaa ttcgctgaag ctgagagctt cttcgccttc ggcaaggcct
     5401 tcgaagtatt cttcgtaagc cttttccatg attgtgtcga aatccatatc acccacctga
     5461 atttctttcc agccagcgac gcgctccaga ttcggtttta aacgttttgc ttttggtata
     5521 cgtcattgcg gtgaaggtgc cgtcctggtt gggaaacacg ccgtacacca gagattcgtt
     5581 gttgccaaga tcgatagtat ccatgctgac ctcatttccc cttaacgccg gggtagcgga
     5641 acaaaaacct gctgcatagt tattaaagtt gaaccctgcc gtcatgttct tacgcctcgg
     5701 gctggctact taacccctga ccactgcctg gtaactcgaa gtattgccct gcattctgtg
     5761 gggcggggtg ggtggcaggt atataatgta ctttgcgttc attgttgtaa agtactttta
     5821 gtacattttg tgtgtaaaaa aatgagatgg gataaagtga agcacaaacc cggaggaagg
     5881 cgctaccgga tttatgctgg tttaagaggc tttttgtttt ttctttcgtg ctaactcttc
     5941 gtaaattgca ttgtacttct gttttttctc ttcaagagtt tttaaaagtt catctgtctc
     6001 actgtcaggg agctcgtcca gaaggtcaat gatgattttt tgtcttggat ttaactcctg
     6061 atagaaacgt acctgtccac tttcttctgt atcctctccc aaaagatagg ttggtgttgt
     6121 tcctattagt gttgctaatt cccttaattt ctcccggcga ggaattgttt cgccattaaa
     6181 ccatttgcta accgcttttg gtgttaattt cattcgacgg gcaatttctg cctgccttcc
     6241 atgttgttca taaccagcgt tttcacaggc tagcgcaagc ctactggcga actctttacg
     6301 cgctttatct tcatgaacca taagttcaat gatattcgct cttgaatgta ctgtcagttc
     6361 tgttatagca tgtactcaaa gttcacactg tgagggtgat atgaaccaga aaacacttga
     6421 agatgtaatc aaaactgttc gcgttgctgt tgtggccgac gtttgtggtg tcagccaaag
     6481 agcaatctat aaatggatgg ataacggaaa attgcctcgc acagaatata ccggcgaaac
     6541 aaattacgct gaaaaaatcg ctcttgcatc aaacggatta ttttctgccg atgcaatttt
     6601 aactattggc aggaataaaa ctactacgaa aaagctgatg ggagttgatt catgaaaatc
     6661 aagcatgaac acatccgcat ggcgatgaat gcctgggcgc atccggacgg cgaaaaagta
     6721 ccggctgcga aaattaccaa agcgtatttc gagctgggaa tgacgttccc ggaactgtat
     6781 gacgacaacc atccggaagc cctggctcgc aatactcaga aaattttccg ctgggtggag
     6841 aaagacaccc ctgatgcggt taaaaaaatt caggcgttgt taccagctat cgaaaaagca
     6901 atgccacctc tgctggtggc ccgaatgcgc agtcatagct cagcatattt tcgggaacta
     6961 gtggagacgc gggaacgact ggtgagagac gctgatgatt ttgtcgcagt ggcgatcgct
     7021 ggtttcaacc agatgaatcg tggtggccct gcaggaaata ttgtggctgt gcattgactc
     7081 gcaatattca taccggatca cttccggcaa tttgtgagta aaaagattcg gtatcaaaag
     7141 aggtgagtat ggctaacgcc tggctcagat tatggcatga catgccaaat gaccctaagt
     7201 ggcgaacaat tgccagggtg tcagggcagc caattgcaac agtgatggca gtgtatatcc
     7261 acctcctggt gagcgcgtca cgaaatgtca cgcgaggtca cattgatgtc acgacagaag
     7321 atttggcaag tgcgctcgac gtgacagaag aggtaattga ttcaattttg cagacgatgc
     7381 aggggcgggt acttgatggt gatttaatca ctggatggga aaaacgccag gtgcttaaag
     7441 aggacaacgg caatatttcg caaaccgcaa aatctcctgc agagcgcaag agggcgcagc
     7501 gagagaggga aagaaagcgg gaacaaaatg gcgattgtca cggcgcgtca cgaaatgtca
     7561 cgcacatgtc acgacgagtc acgacagata aagatacaga taaagataca gatcaagaag
     7621 atcaaaacac tatggtccat ggcgtaaaaa acgccacgaa ccaggcaggg gatgttcaga
     7681 ccgtcaatcc tggtcagcca gcaggcacga caccggaagc cgattcagcg tatgcgctga
     7741 aagccgattc gggcgctgtg cagcaggtga tgaccgcaag accggagcaa tcacaccaac
     7801 tgcagcagcc cgaagccgat tccgccattc agcgggaagc cgatcgggta gtcccggaaa
     7861 acaccgggca gtctgtggga cgagtggatt atccggatgt gttcgaacag gtctggcggg
     7921 agtacccgtt gcgtgctggg gcaaacccga agaaatccgc tttcagtgcc tggaaggcca
     7981 gattacgcga gggggtgcca ccagaggcca tgctggatgg cgtgaggcgt tacgcaagat
     8041 acttggcggc taccgggaaa acgggaacgg aatttgttca gcgagcgacg acgttttttg
     8101 gaccggaccg gaattttgag aatccctggt tgctcccggt aagcggcacg aacaaccagc
     8161 gttgtgtgaa tcatatttct gaaccggata acgaaattcc gccgggcttc agggggtaag
     8221 tgttaatttc tggtcatgag gtaattttca ggagggcttg tggcaaaagt ttttacacaa
     8281 gaagagcggg aaaaaattaa agggcaggtt gttgaactcg tacgccagag tgggcgcgag
     8341 acgttacgac aactggaaac taaaactggg gcaacaagat atctgatgaa cgttctggcc
     8401 agagagctgg ttgccagtgg cgatgtatac aactctggtt acgggttatt cccgtctgaa
     8461 caggcgcgta aggactggca aaatgcccgt aaaaagctct caagggcaaa gctgaagaaa
     8521 ccatctgcgg tcgatccgga ccttatctgg tcattacctg atggagaaat acgtcgttac
     8581 gacaggcgtc ataatatgat ttgtactgag tgtcgtaaaa gcgaagttat gcagcgcata
     8641 ttgtcgtttt atcaggggga tgttcggtat ttattgaagt gacgagatta aagtgcatta
     8701 gttcagatgc aaattgacat tttgtggcac agggtagagc tagcgcggtt gtccgctttg
     8761 tgccaggagc ggaagtagca aaccagattt tactgaagct tgagcagaga taccaccaac
     8821 tgatgcctat cagtaatggg gctcttgatg attcaaataa aaaagattaa attgattaag
     8881 attattctta cttgattaat tgttttgcgc atgggcttgg aaaaccatga atcagatctt
     8941 cactagataa aacaacgaac tatggaggaa gcgtgggcgg cgaaactaag aatattgatg
     9001 agattgcagg tatcatttcc agtagaattt ttgatgagtt aggctggaaa acccaaaata
     9061 caacggatat aagttgggac tgttgtttga gatctcatct aaacgctaaa caacttttat
     9121 ctgaaaaccc aaaaaaaact catcctacgg atgtagtctt caaatataaa gatccttatt
     9181 cagatataac tcagtatata caaacagatt taaaatctta ttgtgccagc acgcttgacg
     9241 g
//
LOCUS       DAHHTG010000160         9224 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.163, whole genome shotgun sequence.
ACCESSION   DAHHTG010000160 DAHHTG010000000
VERSION     DAHHTG010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9224)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9224)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9224
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.163"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            complement(146..1789)
                     /gene="yeeO"
                     /locus_tag="NBI74_004005"
     CDS             complement(146..1789)
                     /gene="yeeO"
                     /locus_tag="NBI74_004005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416491.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic metabolite efflux MATE transporter YeeO"
                     /protein_id="HCD1934264.1"
                     /translation="MLRHILTAKNILSNPIFKFPNCLPFLSTVCCICRQFVGENLCSF
                     ADSPSSFEMWFHFLQLRSALNISSALRQVVHGTRWHSKRKSYKVLFWREITPLAVPIF
                     MENACVLLMGVLSTFLVSWLGKDAMAGVGLADSFNMVIMAFFAAIDLGTTVVVAFSLG
                     KRDRRRARVGTRQSLVIMTLFAVLLATLIHHFGEQIIDFVAGDATTDVKALALTYLEL
                     TVLSYPAAAITLIGSGALRGAGNTKIPLLINGSLNILNIIISGILIYGLFSWPGLGFV
                     GAGLGLTISRYIGAVAILWVLAIGFNPALRISLKSYFKPLNFSIIWEVMGIGIPASVE
                     SVLFTSGRLLTQMFVAGMGTSVIAGNFIAFSIAALINLPGSALGSASTIITGRRLGVG
                     QIAQAEIQLRHVFWLSTLGLTAIAWLTAPFAGVMASFYTQDPQVKHVVVILIWLNALF
                     MPIWSASWVLPAGFKGARDARYAMWVSMLSMWGCRVVVGYVLGIMLGWGVVGVWMGMF
                     ADWAVRAVLFYWRMVTGRWLWKYPRPEPQKCEKKPVVSE"
     gene            1794..1869
                     /locus_tag="NBI74_004006"
     tRNA            1794..1869
                     /locus_tag="NBI74_004006"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1827..1829,aa:Asn,seq:gtt)
     gene            complement(1907..2857)
                     /gene="cbl"
                     /locus_tag="NBI74_004007"
     CDS             complement(1907..2857)
                     /gene="cbl"
                     /locus_tag="NBI74_004007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator Cbl"
                     /protein_id="HCD1934265.1"
                     /translation="MNFQQLKIIREAARQDYNLTEVANMLFTSQSGVSRHIRELEDEL
                     GIEIFVRRGKRLLGMTEPGKALLVIAERILNEASNVRRLADLFTNDTSGVLTIATTHT
                     QARYSLPEVIKAFRELFPEVRLELIQGTPQEIATLLQNGEADIGIASERLSNDPQLVA
                     FPWFRWHHSLLVPLDHPLTQITPLTLESIAKWPLITYRQGITGRSRIDDAFARKGLPA
                     DIVLSAQDSDVIKTYVALGLGIGLVAEQSSSEQEEKNLIRLDTRHLFDANTVWLGLKR
                     GQLQRNYVWRFLELCNAGLSVEDIKRQVMENSEEEIDYQI"
     gene            complement(2959..3876)
                     /gene="nac"
                     /locus_tag="NBI74_004008"
     CDS             complement(2959..3876)
                     /gene="nac"
                     /locus_tag="NBI74_004008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen assimilation transcriptional regulator
                     NAC"
                     /protein_id="HCD1934266.1"
                     /translation="MNFRRLKYFVKIVDIGSLTQAAEVLHIAQPALSQQVATLEGELN
                     QQLLIRTKRGVTPTGAGKILYTHARAILRQCEQAQLAVHNVGQSLSGQVSIGFAPGTA
                     ASSITMPLLQAVRAEFPEVVIYLHENSGAVLNEKLINHQLDMAVIYEHSPVAGVSSQA
                     LLKEDLFLVGTQDCPGQSVDVNAIAQMNLFLPSDYSAVRLRVDEAFSLRRLTAKVIGE
                     IESIATLTAAIASGMGVAVLPESAARSLCGAVNGWMSRITTPSMSLSLSLNLPARANL
                     SPQAQAVKELLMSVISSPVMEKRQWQLVS"
     gene            4203..4278
                     /locus_tag="NBI74_004009"
     tRNA            4203..4278
                     /locus_tag="NBI74_004009"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4236..4238,aa:Asn,seq:gtt)
     gene            complement(4334..5269)
                     /gene="ldtA"
                     /locus_tag="NBI74_004010"
     CDS             complement(4334..5269)
                     /gene="ldtA"
                     /locus_tag="NBI74_004010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HCD1934267.1"
                     /translation="MMRRVNILCSFALLFASQNSLAVTYPLPPEGSRLVGQSLTVTVP
                     DHNTQPLETFAAQYGQGLSNMLEANPGADVFLPKPGSQLTIPQQLILPATVRKGIVVN
                     VAEMRLYYYPPDSNTVEVFPIGIGQAGRETPRNWVTTVERKQEAPTWTPTPNTRREYA
                     KRGESLPAFVPAGPDNPMGLYAIYIGKLYAIHGTNANFGIGLRVSQGCIRLRNDDIKY
                     LFDNVPVGTRVQIIDQPVKYTTEPDGSKWLEVHEPLSRNRAEYESDRKVPLPVTPSLR
                     AFINGQEVDVNRANAALQHRSGMPVQISSGSRQMF"
     gene            complement(5331..6410)
                     /gene="cobT"
                     /locus_tag="NBI74_004011"
     CDS             complement(5331..6410)
                     /gene="cobT"
                     /locus_tag="NBI74_004011"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HCD1934268.1"
                     /translation="MQILADLLNTIPAINSAAMSRAQRHVDGLLKPVGSLGKLEALAI
                     QLAGMPGLNGIPHVGKKAVLVMCADHGVWEEGVAISPKEVTAIQAENMTRGTTGVCVL
                     AAQAGANVHVIDVGIDTAEPIPGLINMRVARGSGNIASAPAMSRRQAEKLLLDVICYT
                     RELAKNGVTLFGVGELGMANTTPAAAIVSTITGRDPEEVVGIGANLPTDKLANKIDVV
                     RRAITLNQPNPQDGVDVLAKVGGFDLVGIAGVMLGAASCGLPVLLDGFLSYAAALAAC
                     QMSPAIKPYLIPSHLSAEKGARIALSHLGLEPYLNMDMRLGEGSGAALAMSIIEAACA
                     IYNNMGELAASNIVLPGNTTSDLNS"
     gene            complement(6422..7165)
                     /gene="cobS"
                     /locus_tag="NBI74_004012"
     CDS             complement(6422..7165)
                     /gene="cobS"
                     /locus_tag="NBI74_004012"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HCD1934269.1"
                     /translation="MSKLFWAMLSFITRLPVPRRWSQGLDFEHYSRGIITFPLIGLLL
                     GAISGLVFMVLQAWCGVPLAALFSVLVLALMTGGFHLDGLADTCDGVFSARSRDRMLE
                     IMRDSRLGTHGGLALIFVVLAKILVLSELALRGEPILASLAAACAVSRGTAALLMYRH
                     RYAREEGLGNVFIGKIDGRQTCVTLGLAAIFAAVLLPGMHGVAAMVVTMVAIFILGQL
                     LKRTLGGQTGDTLGAAIEFGELVFLLALL"
     gene            complement(7162..7707)
                     /gene="cobU"
                     /locus_tag="NBI74_004013"
     CDS             complement(7162..7707)
                     /gene="cobU"
                     /locus_tag="NBI74_004013"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HCD1934270.1"
                     /translation="MMILVTGGARSGKSRHAEVLIGDSSQVLYIATSQILDDEMAARI
                     EHHRQSRPEHWRTVERWQHLDELIHADINPNEAVLLECVTTMVTNLLFDYGGDKDPDE
                     WDYQAMEQAINAEIQSLIAACQRCPAKVVLVTNEVGMGIVPESRLARHFRDIAGRVNQ
                     QLAAAANEVWLVVSGIGVKIK"
BASE COUNT         2555 a         2448 c         1960 g         2261 t
ORIGIN      
        1 atacggatta acagtccgcc gttctaccga ctgaactaca gaggaatcgt gagaacgagg
       61 cgaatattag cgatgcccat ccacaatgtc aaagcctgtt ttttaaattt gaaatcgttt
      121 gctgaaataa tctgcacttt gtcgtttatt ccgacacaac tggctttttt tcacactttt
      181 gcggctcggg tcgagggtat ttccacagcc aacgtccagt aaccattcgc cagtaaaaca
      241 gcacggcccg cacagcccag tcggcaaaca ttcccatcca gacaccaacc acaccccagc
      301 caagcatgat tcccagcaca taaccgacta caacccggca accccacatg ctcaacatag
      361 aaacccacat ggcgtaacgg gcatcacgag cacccttaaa tccagcgggt agcacccatg
      421 aggcggacca aataggcata aataaagcat ttagccaaat cagaatcaca acgacgtgtt
      481 taacctgtgg atcctgggtg taaaacgatg ccataacccc ggcaaaggga gccgttagcc
      541 aggcgatggc cgttaatcca agagtggaca gccagaacac atgccgcaac tgaatctctg
      601 cttgcgctat ctgccctacc cccaaccttc ggcctgtaat gatcgtagaa gcagaaccga
      661 gcgcacttcc gggtaagttg ataagagccg caattgaaaa cgcgataaaa tttccggcaa
      721 taacgctggt ccccatcccg gcaacgaaca tttgggttaa taaccgacca ctggtaaata
      781 gcactgattc gacactcgct ggaataccaa tccccatgac ttcccagata atgctaaaat
      841 tcagcggttt aaaatagctc tttaatgaaa tccttagcgc aggattaaaa ccaatcgcca
      901 gcacccacaa aattgcaact gcgccaatat aacgagaaat ggttaaaccc aacccagccc
      961 cgacaaatcc cagtcccggc caggagaaaa ggccgtaaat caatatgccg ctaataataa
     1021 tattaagaat attcaggcta ccgttaatca gtagcggtat tttcgtattc cctgcaccac
     1081 gaagtgcccc gctaccaata agagtgatgg cagctgctgg ataactgagt acggtcagct
     1141 ccagataagt caacgccagt gctttaacgt ctgtcgtggc atcacccgcg acgaaatcaa
     1201 taatttgttc gccaaaatga tgaattagcg ttgccaacag tacggcaaac aacgtcatga
     1261 tcaccaatga ctgccgcgtc cccaccctcg ctcgtcgtcg atctcgctta ccgagactaa
     1321 atgctacaac gacagtagta ccaagatcga tagctgcaaa aaaagccata atgaccatat
     1381 taaagctgtc cgccaacccc acgccggcca tcgcatcttt tcccagccag ctgaccagaa
     1441 aagtgctcag gacccccatc aacaggacac aggcgttctc catgaagata ggaacagcaa
     1501 gcggggttat ctcgcgccag aacaacactt tgtagctctt gcgtttagag tgccagcgag
     1561 tgccgtgaac aacctggcgt aaagcagagg agatattcaa agccgacctt aattgcagaa
     1621 agtgaaacca catttcaaat gatgagggag aatcagcaaa gctgcaaaga ttttcgccaa
     1681 caaattgtct gcaaatgcaa caaactgttg atagaaacgg caaacagttg ggaaatttaa
     1741 aaatcgggtt tgacaaaata tttttcgccg ttaagatgtg cctcaacagc gattcctctg
     1801 tagttcagtc ggtagaacgg cggactgtta atccgtatgt cactggttcg agtccagtca
     1861 gaggagccaa atttctgaaa agcccgcttt tatagcgggc tttttgctat atctgataat
     1921 caatttcctc ttcactgttt tccatcacct ggcgcttgat atcctcaact gacagtcctg
     1981 cattacaaag ttccagaaag cgccagacat agttacgctg aagttgccct cgcttcagtc
     2041 ccaaccagac agtattagca tcgaaaagat gccgcgtatc caggcggatt aaattctttt
     2101 cctcttgttc gctgctggat tgttcggcaa ctaatccgat cccaagccca agagcaacat
     2161 aggttttaat gacatcagaa tcctgtgcac ttaatacaat atctgccggc aaacctttgc
     2221 gggcaaatgc gtcatcaatg cgtgagcgcc ccgtaattcc ctgtcggtaa gtaattaacg
     2281 gccacttcgc tattgattcc agcgtcaacg gtgtaatttg cgtcaaggga tgatcgagtg
     2341 gaacaagcaa actatggtgc caacgaaacc acgggaaggc gacgagctgc gggtcattac
     2401 tcaaacgctc gctggcgata ccaatatcag cttcgccatt ttgcaacaac gtcgcaattt
     2461 cctgtggtgt accctggatt agctccagcc gaacctccgg gaaaagttcg cgaaaagctt
     2521 taatgacctc aggcaagcta taacgtgcct gagtatgcgt cgttgcaata gtgagaacgc
     2581 cagacgtatc gttggtaaac aggtctgcga gccgacgaac attactggct tcattcagaa
     2641 tgcgttctgc aatgaccagt aatgctttgc ccggttcagt catgcccagc agtcgcttac
     2701 ctcgtcgaac aaatatttcg atgccaagtt catcctccag ttcccgaata tgacggctaa
     2761 cgcctgactg tgaggtaaaa agcatattcg caacctctgt caggttgtaa tcctgacgtg
     2821 cagcctcgcg gattatcttt agttgttgga aattcacggt aaactccggg cagttcagat
     2881 atcccgttat tgttaaagtc taatgcccgg cataacaaat aataaaaacc cgcatcttat
     2941 tccatcccga tataacgctt agctcaccaa ttgccactgc cttttttcca tcactggaga
     3001 actaatcact gacattaaca actctttcac tgcctgtgcc tgtggtgaca agtttgctct
     3061 ggcgggtaaa tttaaggaca aagagagact cattgaagga gtggtaatgc gtgacatcca
     3121 cccatttact gcgccacata acgaacgcgc agccgattca ggtaatactg caacgcccat
     3181 gccgctggca atcgctgcgg taagcgtggc aatagactca atttcaccaa tgacttttgc
     3241 cgtgagccgc cgtagggaaa aagcctcatc aacacgaagt ctaacagcgc tgtaatcact
     3301 ggggagaaaa aggttcattt gcgcaatagc attaacatca acgctttgcc ccgggcaatc
     3361 ttgagttcct accaggaaaa gatcttcttt cagcaaagcc tgactggata ccccagccac
     3421 aggggaatgc tcataaatca ccgccatatc gagttggtga tttatcagtt tttcgttaag
     3481 cactgcacca ctattttcat gaagatagat aacgacctcc gggaattcag cgcgaaccgc
     3541 ctgtaataag ggcatggtga tggatgacgc agcggttcct ggcgcaaagc caatcgagac
     3601 ttgccccgat aatgactgac caacgttatg caccgccagt tgggcctgct cacactgacg
     3661 taaaatggcc cgtgcatggg tatagagaat ttttccggcg cctgttggcg taacgccccg
     3721 ttttgtgcga atcaaaagct gttgatttaa ctcaccttcc agtgtggcaa cctgctggct
     3781 gagcgctggt tgtgcgatat gcaatacttc agcagcctgg gtcaggctac caatatctac
     3841 aatttttacg aagtatttca ggcgtctgaa gttcatgttg cctccggttt ttaagaatcg
     3901 gcccaagtgc cgccattact tacaaccaga atgcaagatg cttgccagtt ctattttagc
     3961 gttgatgcgc aagctaacca actgtcaaat aagagattat gatagattcg tcattggccc
     4021 tttttatcag cagtcgcatt cacctgccct ataaaagggg ggtatgcacc acgatggttc
     4081 attacccaat aagattgaaa gctcaccact ttgttgaaat tgtcagcaaa caaacaaaaa
     4141 aatgcatttc accctttgac atcaccatgc actgccatta atatgcgccc cgttcacacg
     4201 attcctctgt agttcagtcg gtagaacggc ggactgttaa tccgtatgtc actggttcga
     4261 gtccagtcag aggagccaaa ttcaaaaaag cctgctttcg agcaggcttt ttactttcta
     4321 attaccaacg ctcttaaaac atctgtcttg aaccagaact aatttgcaca ggcattcccg
     4381 atcgatgttg caacgcagca tttgcgcgat ttacatcaac ttcttgcccg ttgataaacg
     4441 cccgcaaaga tggggttacc ggcaatggca cttttcggtc agactcatat tctgcacgat
     4501 tgcgcgacag cggctcatga acttccagcc actttgaacc atccggttcc gttgtgtatt
     4561 tcactggctg atcaataatt tgtacacgag tgcctacagg aacattatca aacagatatt
     4621 tgatatcgtc attacgcaga cgaatacagc cctgacttac acggagccca ataccaaaat
     4681 tggcattggt accgtggatg gcatacaact tgccaatata aatcgcgtac agccccatgg
     4741 ggttatcggg ccccgcagga acaaatgcgg gcaaactctc ccctcgtttc gcatattcgc
     4801 gccgggtgtt cggcgttggc gtccaggttg gtgcttcttg tttacgttca acggtagtca
     4861 cccagttacg cggggtttct cgcccagcct ggccgatacc aataggaaag acttccactg
     4921 tattactgtc tggcggatag taataaagac gcatttcagc gacgttaacc acaatccctt
     4981 tacgaacagt ggcgggcaaa atcagttgct gcggaatggt gagttgtgag ccaggcttcg
     5041 gcaaaaaaac atcagcgccc ggattcgctt ccagcatatt acttaaccct tgcccgtatt
     5101 gtgcggcaaa agtctccagc ggctgagtat tgtgatcagg aacagttaca gtaagcgact
     5161 gccccactaa acggctaccc tctggaggta atggataagt taccgccagg ctattttggc
     5221 tggcaaaaag cagagcaaat gagcaaagaa tatttacacg acgcatcatg tccctttcct
     5281 aagtcgcgaa tactatccga taagtatagc ttttatcaga cttttcgttt ttagctgttc
     5341 aaatcagaag tcgtattccc cggtagaaca atattactgg cagcaagttc gcccatgttg
     5401 ttgtatatcg cacaagcagc ttcgatgatg gacatcgcca gagctgcacc actcccctca
     5461 cctaaacgca tatccatatt gagataaggc tccagcccca aatgcgagag cgctatacgc
     5521 gcgccttttt ctgccgacaa gtgagaagga atgagatacg gtttgattgc aggagacatc
     5581 tggcaggctg cgagcgcagc agcataagaa agaaatccat ccagcaacac gggtaaaccg
     5641 caggaagcag cacctaacat cactccagct attccgacca aatcaaatcc acccactttt
     5701 gccaggacat caacaccatc ctgaggattt ggttgattca acgtaatcgc ccgacgcaca
     5761 acatcaattt tattagccag tttatctgtc ggcaggtttg cgccaatccc aaccacttct
     5821 tcaggatccc ggccagtgat tgtgctgact attgccgctg ccggtgtcgt gtttgccatc
     5881 cccagttcac ctacaccaaa cagcgtgaca ccgttttttg ccagctcccg cgtataacat
     5941 atgacgtcca aaagcaactt ttcagcctga cggcgactca ttgccggagc tgaagcaata
     6001 ttgccgctac ctcgtgcgac acgcatgttg ataagcccgg ggataggctc agcagtatca
     6061 ataccaacat caattacgtg gacgttagcg cccgcttgtg ctgccagcac acacacgccg
     6121 gttgttccac gggtcatatt ttcagcctgt atggctgtca cttcttttgg agaaatagcg
     6181 accccttcct cccagacgcc gtgatcggca cacataacca gtactgcttt tttgcccaca
     6241 tgcggtatgc cattcaaccc cggcattcct gccagttgta tggcaagcgc ctccagcttt
     6301 cccaggctac caacaggttt gagtaacccg tcaacatgcc gttgtgcacg cgacatagcg
     6361 gcagaattga tggctgggat cgtattcagt aaatcggcaa gtatttgcat gtcacgtctc
     6421 tttatagcag tgccagcagg aaaaccagtt caccaaactc tatggctgct cccagcgtat
     6481 cgcccgtttg cccgcccagc gtacgtttga gaagctgacc gaggatgaaa atcgccacca
     6541 tcgtcaccac catagcagcc acaccatgca tacccggcaa taatacggca gcaaaaatag
     6601 cggctaagcc gagtgtgacg caggtttgtc gcccatcaat tttgccaatg aacacattgc
     6661 caagcccttc ttcccgcgca taacgatgac gatacatcaa taatgcggca gtcccgcggc
     6721 tgaccgcaca tgctgccgcc agcgatgcaa ggatcggttc gccacgcaga gccaactcgc
     6781 ttaacaccag aatctttgcc agtaccacaa aaatcaatgc cagaccgccg tgggtgccta
     6841 aacggctatc acgcatgatt tccaacatgc gatcgcggct acgtgcagaa aatacgccat
     6901 cacaggtatc agcgaggccg tccagatgga atcccccggt catcagcgcc agcacaagta
     6961 cgctaaacag tgccgccagt ggtacgccac accatgcctg cagcaccatg aagaccagcc
     7021 cgctaatcgc gccaagcaat aatccaatca aaggaaaagt aataataccg cgagaataat
     7081 gctcgaaatc cagtccctgg gaccagcgac gcggtacagg cagacgcgta ataaatgaga
     7141 gcatcgccca aaataattta ctcatttgat ttttactcca atacccgaaa ccaccagcca
     7201 tacttcattt gccgcagcgg ccaactgctg atttacccgc ccggcaatat cacgaaaatg
     7261 tcgtgccaga cgactctccg gcacaatccc cattcccact tcgttagtca ctaatacaac
     7321 ctttgcgggg caacgttggc aggcagcaat caacgactga atctcagcat taatcgcctg
     7381 ttccatcgcc tgataatccc attcatcagg gtctttatcg ccgccataat caaacaacag
     7441 attagtcacc attgtggtaa cgcattccag caacacagcc tcattcgggt taatgtctgc
     7501 atgaattaat tcatcaagat gttgccagcg ctccactgtg cgccagtgct ccgggcggct
     7561 ttgccgatga tgttctatcc gtgcagccat ctcatcatca aggatttgcg aggtagcgat
     7621 atacagaacc tgtgaagagt ccccaataag cacctctgcg tggcgactct tcccgctccg
     7681 tgcgcctccc gtcaccaaaa tcatcatacc gactcctgat tcaaatgtat gatggtattc
     7741 attgaacaga gcaatggatc ccccaccgtt gctgaccgca aaacatttac gcgattaata
     7801 ctgcgcgtaa tataattaaa aatccagcat tctcaatcca ttacgaaata agatggcatt
     7861 acggaatagc cagactttct cgtgttaacg tccgacaaaa tgcgtcattg acagcggttt
     7921 ctgtggattg acacatttca ttcagataat gaattaatgc tactgccgga ccaaccagca
     7981 aacgggcatt gtgttctgaa atccaggcta ttgattcaac gtcagcgaca gatctgcgct
     8041 gtaaaagaag ttgtaaaaca acgcaataaa attatccgga atggtggcga ctatgcacta
     8101 gaacaccaca atttagctct ctcggtaagg cactgtcaca gcattcaaca gaatgtgact
     8161 tgccagattt attagcgcca ccagatgttt aaccgggtaa ccacgaccca gtcggggata
     8221 tattccatct tctcgctgac atcatcaaga ataaaaaggt ttatcacact cagagcaacg
     8281 ttcttcagca ggcgggataa ttcttcaaca tttaaaagat gtgttattaa aggcatacaa
     8341 actggacgta ttgtattttc ttttggtggt tgacctaagg tagcagttta tcctgatgcg
     8401 ctgagatttc tgtaatatct acgtcaacat tctcctcgaa tagaaaatgc agcctcggca
     8461 agtgattcac attttttaac aaagcgttgt tagcgtgcca ttttcagaag ctctccaaga
     8521 aagcgaattc ttttctcaaa ttctgcattc tcagacacag ctttctactc catggcaatg
     8581 gcccaaatac gaagttgctc aggatcatta gtggtgctga gaagttaaat aaccatgcgg
     8641 agtaccactc gtcatcacgt atttatatat catgaagcta ttgttgtgtt ttgtacatca
     8701 gtagatgtat tctgatatac tcctttttct agacataacc tttcacctgc ttgcaaagct
     8761 tctgtgttct gacattgcca aattgttgca attctgtatc cagccttctt tcagtcatag
     8821 cttcgggccg cgataagact cactgatctg accctgattc ctcttgcaga ctttatagac
     8881 caattaaaat gcagtttctg caggtcaacg tctgaccatc attgtcatca ctctggccat
     8941 tagagtaacc ttctgcattc atccttttgt aaaaagttta tgtcagtatc agcaattaac
     9001 cggacctgat actgatatga gtcttaccgc atatacggtc aatttcagca attaattaca
     9061 ttatccacgc caaagtgttt gtcatcgcaa tgatggtacc ttctttcaga caccattttt
     9121 tcaactccgt tttccacgga ccgcactctt atgtcaagag tgcggtccgt ggatacaacc
     9181 agagaccgac tgacacgagt cagaggaaac gacggatatg ttca
//
LOCUS       DAHHTG010000161         9036 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.164, whole genome shotgun sequence.
ACCESSION   DAHHTG010000161 DAHHTG010000000
VERSION     DAHHTG010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9036)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9036)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9036
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.164"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            104..646
                     /gene="ecpR"
                     /locus_tag="NBI74_004014"
                     /gene_synonym="matA"
     CDS             104..646
                     /gene="ecpR"
                     /locus_tag="NBI74_004014"
                     /gene_synonym="matA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECP biosynthesis operon DNA-binding
                     transcriptional regulator EcpR"
                     /protein_id="HCD1934271.1"
                     /translation="MECQNRSDKYIWSPHDAYFYKGLSELIVDIDRLIYLSLEKIRKD
                     FVFINLNTDSLTEFINRDNEWLSAVKGKQVVLIAARKSEALANYWYYNSNIRGVVYAG
                     LSRDIRKELAYVINGRFLRKDIKKDKITDREMEIIRMTAQGMLPKSIARIENCSVKTV
                     YTHRRNAEAKLYSKLYKLVQ"
     gene            721..1308
                     /gene="ecpA"
                     /locus_tag="NBI74_004015"
                     /gene_synonym="matB"
     CDS             721..1308
                     /gene="ecpA"
                     /locus_tag="NBI74_004015"
                     /gene_synonym="matB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="common pilus major fimbrillin subunit EcpA"
                     /protein_id="HCD1934272.1"
                     /translation="MKKKVLAIALVTVFTGTGVAQAADVTAQAVATWSATAKKNTTSK
                     LVVTPLGSLAFQYAEGIKGFNSQKGLFDVAIEGDSTATAFKLTSRLITNTLTQLDTSG
                     STLNVGVDYNGAAVEKTGDTVMIDTANGVLGGNLSPLANGYNASNRTTAQDGFTFSII
                     SGTTNGTTAVTDYSTLPEGIWSGDVSVQFDATWTS"
     gene            1365..2033
                     /gene="ecpB"
                     /locus_tag="NBI74_004016"
                     /gene_synonym="yagY"
     CDS             1365..2033
                     /gene="ecpB"
                     /locus_tag="NBI74_004016"
                     /gene_synonym="yagY"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone EcpB"
                     /protein_id="HCD1934273.1"
                     /translation="MKKHLLPLALLFSGISPAQALDVGDISSFMNSDSSTLSKTIQNS
                     TDSGRLINIRLERLSSPLDDGQVIAMDKPDELLLTPASLLLPAQASEVIRFFYKGPAD
                     EKERYYRIVWFDQALSDAQRDNANRSAVATASARIGTILVVAPRQANYHFQYANGSLT
                     NTGNATLRILAYGPCLKAANGKECKENYYLMPGKSRRFTRVDTADNKGRVALWQGDKF
                     IPVK"
     gene            2059..4584
                     /gene="ecpC"
                     /locus_tag="NBI74_004017"
                     /gene_synonym="yagX"
     CDS             2059..4584
                     /gene="ecpC"
                     /locus_tag="NBI74_004017"
                     /gene_synonym="yagX"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher EcpC"
                     /protein_id="HCD1934274.1"
                     /translation="MPLRRFSPGLKAQFAFGMVFLFVQPDASAADISAQQIGGVIIPQ
                     AFSQALQDGMSVPLYIHLAGSQGRQDDQRIGSAFIWLDDGQLRIRKIQLEESEDNASV
                     SEQTRQQLMTLANAPFNEALTIPLTDNAQLDLSLRQLLLQLVVKREALGTVLRSRSED
                     IGQSSVNTLSSNLSYNFGVYNNQLRNGGSNTSSYLSLNNVTALREHHVVLDGSLYGIG
                     SGQQDSELYKAMYERDFAGHRFAGGMLDTWNLQSLGPMTAISAGKIYGLSWGNQASST
                     IFDSSQSATPVIAFLPAAGEVHLTRDGRLLSVQNFTMGNHEVDTRGLPYGIYDVEVEV
                     IVNGRVISKRTQRVNKLFSRGRGVGAPLAWQIWGGSFHMDRWSENGKKTRPAKESWLA
                     GASTSGSLSTFSWAATGYGYDNQAVGETRLTLPLGGAINVNLQNMLASDSSWSNIASI
                     SATLPGGFSSLWVNQEKTRIGNQLRRSDADNRAIGGTLNLNSLWSKLGTFSISYNDDR
                     RYNSHYYTADYYQSVYSGTFGSLGLRAGIQRYNNGDSSANTGKYIALDLSLPLGNWFS
                     AGMTHQNGYTMANLSARKQFDEGTIRTVGANLSRAISGDTGDDKTLSGGAYAQFDARY
                     ASGTLNVNSAADGYINTNLTANGSVGWQGKNIAASGRTDGNAGVIFDTGLENDGQISA
                     KINGRIFPLNGKRNYLPLSPYGRYEVELQNSKNSLDSYDIVSGRKSHLTLYPGNVAVI
                     EPEVKQMVTVSGRIRAEDGTLLANARINNHIGRTRTDENGEFVMDVDKKYPTIDFRYS
                     GNKTCEVALELNQARGAVWVGDVVCSGLSSWAAVTQTGEENES"
     gene            4574..6217
                     /gene="ecpD"
                     /locus_tag="NBI74_004018"
     CDS             4574..6217
                     /gene="ecpD"
                     /locus_tag="NBI74_004018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial adhesin EcpD"
                     /protein_id="HCD1934275.1"
                     /translation="MRVNLLIAMIIFALIWPATALRAAVSKTTWADAPAREFVFVENN
                     SDDNFFVTPGGALDPRLTGANRWTGLKYNGSGTIYQQSLGYIDNGYNTGLYTNWKFDM
                     WLENSPVSSPLTGLRCINWYAGCNMTTSLILPQTTDASGFYGATVTSGGAKWMHGMLS
                     DAFYQYLQQMPVGSSFTMTINACQTSVNYDASSGARCKDQASGNWYVRNVTHTKAANL
                     RLINTHSLAEVFINSDGVPTLGEGNADCRTQTMGSRSGLSCKMVNYTLQTNGLSNTSI
                     HIFPAIANSSLASAVGAYDMQFSLNGSSWKPVSNTAYYYTFNEMKSADSIYVFFSSNF
                     FKQMVNLGISDINTKDLFNFRFQNTTSPESGWYEFSTSNTLIIKPRDFSISIISDEYT
                     QTPSREGYVGSGESALDFGYIVTTSGKTAADEVLIKVTGPAQVIGGRSYCVFSSDDGK
                     AKVPFPATLSFITRNGATKTYDAGCDDSWRDMTDALWLTTPWTDISGEVGQMDKTTVK
                     FSIPMDNAISLRTVDDNGWFGEVSASGEIHVQATWRNIN"
     gene            6186..6896
                     /gene="ecpE"
                     /locus_tag="NBI74_004019"
     CDS             6186..6896
                     /gene="ecpE"
                     /locus_tag="NBI74_004019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308346.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone EcpE"
                     /protein_id="HCD1934276.1"
                     /translation="MFRRRGVTLTKALLTVVCMLAAPLTQAISVGNLTFSLPSETDFV
                     SKRVVNNNKSARIYRIAISAIDSPGSSELRTRPVDGELLFAPRQLALQAGESEYFKFY
                     YHGPRDNRERYYRVSFREVPTRNLTKRSPSGGEVSTEPVVVMDTILVVRPRQVQFKWS
                     FDQVTGTVSNTGNTWFKLLIKPGCDSTEEEGDAWYLRPGDVVHQPELRQPGNHYLVYN
                     DKFIKISDSCPAKPPSAD"
     gene            7203..7532
                     /locus_tag="NBI74_004020"
     CDS             7203..7532
                     /locus_tag="NBI74_004020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HCD1934277.1"
                     /translation="MSNLNPCMTCGACCAFFRVSFYWAEADDAGGTIPARLTEQISPF
                     HRCMSGTNQKNPRCIALAGTPGKNACCTIYENRSSTCREFAMSGENGEVNEACNRARA
                     KYGLTPL"
     gene            complement(7544..7707)
                     /locus_tag="NBI74_004021"
                     /pseudo
     CDS             complement(7544..7707)
                     /locus_tag="NBI74_004021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309678.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
     gene            complement(7781..8395)
                     /locus_tag="NBI74_004022"
     CDS             complement(7781..8395)
                     /locus_tag="NBI74_004022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YagU family protein"
                     /protein_id="HCD1934278.1"
                     /translation="MNIFEQTPPNRRRYGLAAFIGLIAGVVSAFVKWGAEVPLPPRSP
                     VDMFNAACGPESLIRAAGQIDCSRNFLNPPYIFLRDWLGLTDPNSAVYTFAGHVFNWV
                     GVTHIIFSIVFAVGYCVVAEVFPKIKLWQGLLAGALAQLFVHMISFPLMGLTPPLFDL
                     PWYENVSEIFGHLVWFWSIEIIRRDLRNRITHEPDPEIPLGSNR"
     gene            complement(8610..8795)
                     /locus_tag="NBI74_004023"
     CDS             complement(8610..8795)
                     /locus_tag="NBI74_004023"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1934279.1"
                     /translation="MASGLICHGGFEPHAWGVASLMDVTVLVVASLLVWLLVNRENHY
                     ARGIPQDVQVLHKKAHH"
     gene            8844..9002
                     /locus_tag="NBI74_004024"
                     /pseudo
     CDS             8844..9002
                     /locus_tag="NBI74_004024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706266.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
BASE COUNT         2421 a         2293 c         2252 g         2070 t
ORIGIN      
        1 tccgatttac taaaaaaact gaatgtacct gtaaaaatta caggtttgga aagtagtgac
       61 atggcaaaat gattacagca gggactatga ggttaaaaac catatggaat gtcaaaaccg
      121 ttctgataaa tacatctggt ctccccatga cgcctacttc tataaaggac tatctgaact
      181 gattgtggat atcgacagat taatttatct atcgctggag aaaatcagaa aagatttcgt
      241 gtttatcaat ctcaatacgg attctttaac tgagtttata aaccgtgata atgagtggtt
      301 atccgcggta aaggggaaac aggtcgtatt gattgcggcc agaaagtcag aagccttagc
      361 aaattattgg tattacaaca gcaatattag gggcgtggta tacgctggac tgagtcgtga
      421 tattagaaaa gaactggcct atgtgattaa tggcaggttc ctgagaaaag atattaagaa
      481 agataaaatc actgaccggg aaatggaaat tatccgcatg acggctcagg gaatgctgcc
      541 taaatcgatt gccagaattg aaaattgtag tgtgaagaca gtgtataccc atcggcgtaa
      601 tgcagaggcc aagctgtact caaaattata taagttggtt cagtaaactc caggcaagtt
      661 agttttaaaa aatgactcac cgggacttca tgtcctcaat tcaactcggg aagaaaagca
      721 atgaaaaaaa aggttctggc aatagctctg gtaacggtgt ttaccggtac aggtgtggcg
      781 caggctgctg acgtaacagc tcaggctgta gcgacctggt cagcaacagc caaaaaaaac
      841 accaccagta agctggttgt gacgccactc ggtagcctgg cgttccagta tgccgaaggc
      901 attaaaggtt ttaactcaca aaaaggtcta tttgacgtgg ctatcgaggg tgactcaacg
      961 gctaccgcct ttaaactgac ctcacgtctt atcaccaaca ccttaaccca gttggatacc
     1021 tcaggttcca cactgaatgt gggcgtggat tataacggcg cggcagtcga aaaaactggc
     1081 gataccgtga tgatcgatac cgccaacggc gtactgggcg gcaaccttag cccactggct
     1141 aacggttaca atgccagcaa tcgtaccact gcacaggatg gtttcacttt ctccatcatc
     1201 agcggcacca ccaatggtac caccgcagta accgattaca gcactctacc ggaaggcatc
     1261 tggagcggcg acgttagcgt acagttcgac gctacctgga ccagttaatc tctctgatgt
     1321 accagcaggg gtacccccct gctttattcc tggacggaat tgttatgaaa aagcaccttc
     1381 tgcctctcgc tctgctgttt tccggaatat caccggccca ggcgctggat gtcggcgata
     1441 tatcatcgtt tatgaacagt gacagcagca cgctgagcaa aacgatccaa aacagtaccg
     1501 acagtggccg cctcatcaat atccgtctcg aacggctctc ttcaccgctt gacgacgggc
     1561 aggttatcgc aatggacaag ccggatgagt tgctactcac tcccgccagc ttgctgctac
     1621 ccgcccaagc cagcgaagtg atccgcttct tctataaggg accagcagat gaaaaagagc
     1681 gctactaccg cattgtctgg tttgatcagg ccctcagtga tgcgcagcgc gataatgcca
     1741 accgcagcgc tgtggccact gcctccgccc gcatcggcac catcctggtc gtcgcacccc
     1801 gccaggcaaa ctaccacttt cagtacgcca acggctccct gacaaataca ggaaatgcga
     1861 cgctgcggat cctcgcctac ggcccctgcc tgaaagccgc caatggtaag gagtgtaaag
     1921 agaattacta cctgatgccg ggcaagtcgc gtcgttttac ccgcgtggac acggcggata
     1981 acaaaggacg ggttgcactt tggcagggtg ataagttcat tcccgtgaaa tagatagctg
     2041 tgcagatgga taacgacaat gcctttacga cggttctccc caggactgaa agcccagttt
     2101 gccttcggca tggtcttttt gttcgttcag cccgatgcca gcgctgctga cataagtgcg
     2161 cagcaaatag gtggggtgat tattccgcag gccttcagtc aggcgctcca ggacggcatg
     2221 agcgtcccgc tttatattca tctcgccggt agccagggtc gccaggacga tcagcgaatc
     2281 ggcagcgctt ttatctggtt ggatgatgga cagttacgca tccgaaaaat acagctggaa
     2341 gagagtgaag ataacgccag tgtcagcgaa caaactcgac agcagctgat gactctggcc
     2401 aacgccccgt tcaatgaggc ccttaccatc cccctgactg acaacgcgca gttggatctc
     2461 agcttgcgcc aactgctgct gcagctggtg gtcaagcgcg aagcgctggg caccgtacta
     2521 cgctcacgta gcgaagacat cgggcagtcc agtgttaaca ccctcagcag taatctgagc
     2581 tataacttcg gcgtctataa caaccagttg cgtaacggcg ggagcaacac ctccagctat
     2641 ctgtcgctga ataacgttac tgcgctgcgc gaacatcacg tggtgctcga cggctcactg
     2701 tacgggattg gtagcggtca acaggacagt gaattatata aagcgatgta tgaacgcgat
     2761 tttgctggtc accgatttgc cggtggaatg ctcgacacct ggaacttgca gtccttaggg
     2821 ccgatgaccg ccatttcagc agggaagatt tacggccttt cctggggaaa ccaggccagc
     2881 tccaccatct tcgacagcag ccagtcggcc acgccagtga tcgccttttt accggcggcg
     2941 ggcgaagtac atctcacccg tgatgggcgg ctactaagcg ttcagaactt caccatgggc
     3001 aatcatgaag tggatacccg gggtctaccg tacggtattt acgatgtgga agttgaggta
     3061 atcgttaacg gtcgcgtgat cagcaaacgc acccagcggg tcaataagct gtttagccgg
     3121 gggcgtggcg tcggtgcacc gctggcgtgg cagatatggg gcggtagctt tcatatggat
     3181 cgctggtcgg aaaacgggaa aaagacgcga ccagccaaag agagttggct ggcaggtgcc
     3241 tcgacctccg gctcactgag tacgtttagc tgggcggcaa cgggatatgg atacgataat
     3301 caggcggtgg gtgaaacccg tctgacgctt ccgcttgggg gggcgatcaa cgttaacttg
     3361 caaaacatgc tggccagtga cagctcatgg agcaacatcg ccagcatcag cgccactcta
     3421 cctggaggct tcagttcgct gtgggttaac caggaaaaaa cccgcattgg caatcaattg
     3481 cgacgtagcg atgccgacaa ccgtgcaatc ggcggcacac tcaacctgaa ctcactgtgg
     3541 tcgaagctgg gtacgttcag catcagctac aatgatgacc gccgctacaa cagccactat
     3601 tacacggcag attactatca aagtgtctac agcggtacct ttggttcgct tggcctgcgg
     3661 gccggtattc agcgctataa caacggcgac agcagcgcca atacagggaa atatatcgct
     3721 ctcgatctct cgctaccact gggcaactgg tttagcgcag ggatgaccca tcaaaacggc
     3781 tacaccatgg caaacctgtc agcacgcaaa cagtttgatg aaggaaccat tcgcactgtt
     3841 ggtgccaatc tgtcacgagc catctccggc gataccggtg atgacaaaac cctcagcggt
     3901 ggggcgtatg cacagttcga cgctcgttac gccagcggaa cgctgaacgt caatagcgcg
     3961 gcggacggct acatcaatac taatttgacc gccaacggca gcgtcggctg gcagggtaaa
     4021 aacatcgctg ccagcgggcg gaccgatggc aacgctgggg tgatattcga cactggcctg
     4081 gagaacgatg gtcagatcag cgccaaaatc aacgggcgga ttttcccgct taacggcaag
     4141 cgtaactatc tcccgctctc tccctatgga agatatgagg tggagttgca gaacagcaaa
     4201 aactcactcg acagttacga tatcgtcagc ggtcgcaaaa gtcatctgac tctctatcca
     4261 ggcaatgtcg ctgtcattga gccagaggtg aagcagatgg ttaccgtctc cggtcgtatc
     4321 cgtgcggaag acggcacact gctggctaac gcacggatta acaaccatat cggccgaacc
     4381 cgaaccgatg aaaacggcga gtttgtcatg gacgtggata agaaataccc cactatcgat
     4441 ttccgctaca gtggcaataa aacctgcgaa gtggcactgg aactcaacca ggcgcgcggt
     4501 gccgtctggg tcggtgatgt ggtctgcagc ggcctctcat cgtgggcggc ggtgacgcag
     4561 acaggagaag agaatgagag ttaacctact gatagcgatg ataatctttg cgctaatctg
     4621 gcctgcaacc gcgctcagag cggcagtgag caaaacaacc tgggcggatg ctccggcacg
     4681 cgagtttgtg tttgtcgaaa acaactcaga cgacaacttt ttcgtcactc ctggcggggc
     4741 gctggatccg cgcctgaccg gtgccaaccg ctggaccggt ttaaaataca atggttcagg
     4801 aaccatctat cagcaaagcc tcggctacat tgataacggt tacaacaccg gcctttatac
     4861 caactggaag tttgatatgt ggctggaaaa ttcaccagtt tcatctcctt taactggctt
     4921 gcgctgcatc aactggtacg cagggtgtaa tatgaccacc agtcttatcc tgccgcaaac
     4981 caccgacgcc agtggatttt atggcgcgac ggtgaccagc ggcggcgcga agtggatgca
     5041 cggcatgttg tcagacgcgt tttaccagta tctgcaacaa atgcccgtcg gcagcagctt
     5101 tacaatgacc atcaatgcct gccagacctc tgtgaactat gacgccagca gcggcgcacg
     5161 ctgtaaggat caggcctccg gcaactggta tgttcgcaac gtcacccata cgaaagcagc
     5221 aaatctgcgg ttgataaata cccactcgct ggcggaagta tttatcaaca gcgacggagt
     5281 accgactctg ggcgaaggga acgccgactg ccggacgcaa accatgggca gccgttcagg
     5341 attaagttgt aagatggtta actataccct gcaaacaaac ggactcagca acacctcaat
     5401 ccatatattc ccggcgatcg ccaactcgtc gttagcctcg gccgtcgggg cgtacgatat
     5461 gcagttcagt ctgaatggca gttcatggaa accggtgagc aatactgcct attactacac
     5521 cttcaacgag atgaagagcg cagactcgat ctatgtgttc ttctcgagca acttctttaa
     5581 gcagatggtg aacctcggga tcagcgatat caacaccaaa gatctgttca actttcgctt
     5641 tcagaacacc acatcaccgg agtctggctg gtatgaattt tctacctcca acacgctgat
     5701 tatcaaaccc cgtgatttca gcatcagtat tatctccgat gaatatactc agacaccgtc
     5761 gcgggaggga tatgtcggca gcggcgagtc ggcactcgat ttcggctata tcgtaaccac
     5821 cagcggtaaa acagctgccg acgaagtgct gatcaaggtg accggacccg cgcaggtgat
     5881 tggcgggcgc tcctattgtg tcttcagctc cgatgacggt aaggcgaaag taccgttccc
     5941 ggcgacgctt tcctttatta cccgcaacgg agctacaaaa acctacgatg cagggtgcga
     6001 tgatagctgg cgggatatga ccgatgcgct gtggttgacc acaccgtgga ctgatatctc
     6061 tggcgaagtg gggcagatgg ataagaccac agtcaaattt tcgattccaa tggataacgc
     6121 catttctctg cgtacggtag atgataacgg ctggtttggc gaagtcagcg cttcaggaga
     6181 gattcatgtt caggcgacgt ggcgtaacat taactaaggc cctgctgaca gtggtctgta
     6241 tgctggcggc acctttgaca caggcgattt cggtcggcaa tctgactttt tcactgccgt
     6301 ccgagactga ctttgtcagc aaacgtgtcg tgaataacaa caaaagcgca cggatatacc
     6361 gtattgccat cagtgctatt gatagcccgg gcagcagtga attgcgcacc cgaccggtgg
     6421 atggtgaact acttttcgcc ccccgccagc tggcgttgca ggctggtgag agcgagtatt
     6481 ttaaatttta ctatcatggc ccacgggata accgcgagcg ctactaccgg gtctcatttc
     6541 gcgaggtccc cactcgtaac ctgacaaaac gcagcccaag cggcggtgag gtcagcacgg
     6601 agccggtggt ggtgatggat accattctgg tagtacgacc acgtcaggtt cagtttaaat
     6661 ggtccttcga tcaggtgaca ggaacggtaa gtaacaccgg caacacgtgg tttaagctac
     6721 tgattaaacc aggatgtgat tcgaccgaag aggaaggcga tgcctggtat ctacgtccgg
     6781 gagacgtggt tcatcagcct gagttacgtc agccggggaa tcattatctg gtctataacg
     6841 acaaattcat taagattagc gattcctgtc cggctaagcc cccttcggcg gactaagact
     6901 tatccatcgg acgagggaag aattccacct cagagactca aaaatcgttt aaatgatgtg
     6961 gaagcgatcg tcaatgtcac cgatgagctt tctcatatgc acaccacagt gttcaggaga
     7021 cacggaaagg taccaggcgg cctgctaacg agtggtttct tcactaatta ggacgtaaaa
     7081 tcggtaacgg ctggaaatca ttcaatactc acactatcga aagtacacca gccaaccgcg
     7141 atacgtccct gcatacgacg taccgcagtt atctttacga tttatagctg tactggtgaa
     7201 ttatgagcaa tttgaatcca tgcatgacgt gtggtgcctg ttgtgcattt ttccgcgtct
     7261 ctttttactg ggccgaagcc gacgatgctg gcggtaccat tcccgccagg ctcactgaac
     7321 aaatatcccc ttttcaccga tgcatgagcg gtactaatca gaaaaatccc cgatgtattg
     7381 cccttgcagg aacaccgggc aaaaatgcct gctgcacgat atatgaaaat cgatcgtcca
     7441 catgcagaga attcgccatg tctggtgaga acggagaagt caatgaggct tgcaatcgtg
     7501 caagagctaa atacgggcta acaccgttat aaatatacaa taattaattg cattgccccg
     7561 ccagctgtaa tagcggggct tcgtttttca gggacaacaa aacctgaaac ttcttccatt
     7621 ttccagcaac aatgcctctt cttctgtagc gtcctccgga tctacatcat cctgaagtag
     7681 cgcgatatga tcactgactg tccgcatcag aatatgaaaa gcaacgtttt taacctataa
     7741 aaatggcgct atatttgcgc catttttatc attcaaagca ttatctattt gagcctaaag
     7801 ggatctcagg atctggctca tgggtaattc tgtttcgtaa atctctgcga ataatttcaa
     7861 tagaccagaa ccagactaaa tgtccaaaaa tttcagagac attctcatac cacggaagat
     7921 caaagagagg tggcgtaagt cccatgagag ggaatgaaat catatgaaca aaaagttgcg
     7981 ctaaagcacc tgccagtaag ccctgccaaa gtttaatttt cggaaatact tcggcgacca
     8041 cacagtaacc aacggcaaaa actatcgaaa agattatgtg cgttacacca acccagttaa
     8101 agacatgccc ggcgaaggta taaacagccg aatttggatc tgtcagtccc aaccaatccc
     8161 gaagaaaaat gtacggagga ttgagaaagt tacgggagca atcaatttgg cctgcagccc
     8221 tgattaatga ttccggacca cacgctgcat taaacatatc aaccggacta cgtggtggca
     8281 atggaacttc tgccccccac ttaacaaatg cggaaacaac gccagcaatc agcccaatga
     8341 atgcagcaag accataacgt ctgcggtttg gtggagtttg ttcaaatata ttcatatcta
     8401 ccctgcttgt accattatgt tatacacctc tttaggagta ttcataaaac aaggcaaatg
     8461 taaagaactg tattgttttg tataaccaca tggttaccta atcgccaatt aatataagct
     8521 ccattatttc tccatatttt tatgttagaa gtgacaaaga gaaatgtaat gtttctctca
     8581 gagaatataa atcattcctt ttgcccacct taatggtggg cttttttatg caaaacctga
     8641 acgtcctgtg gtattcctct ggcataatga ttttcgcgat taaccaacaa ccagaccagc
     8701 aaagacgcca ccacaagcac agtaacatcc atcaggcttg ccacacccca tgcgtgcggc
     8761 tcaaagccgc catggcatat taatccgctt gccattttcg aacagctgat tggtttggtc
     8821 cggtgtttcc ctgcttgcac ttaaaaatga gcggatagga aactctcgaa atggttcgtc
     8881 gatatgctca cctcgcacct aatcacctta ccgaacacgc acggtaaata gattcgatcc
     8941 tgaacccatc ggtcccaaat ctgtcccaat caaaaaataa ggaaggtatt aatgatgtgt
     9001 aacttattga tttaagtggt gccgataata ggagtc
//
LOCUS       DAHHTG010000162         8872 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.165, whole genome shotgun sequence.
ACCESSION   DAHHTG010000162 DAHHTG010000000
VERSION     DAHHTG010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8872)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8872)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8872
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.165"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            complement(<1..493)
                     /locus_tag="NBI74_004025"
     CDS             complement(<1..493)
                     /locus_tag="NBI74_004025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCD1934280.1"
                     /translation="MMSMRKHIAFASMCMGLFIAQLDIQIVSSSLNEIGGGLSAGKDE
                     MAWLQTSYLIAEIIVIPLSGWLSRVFSTRWLFTLSAGIFTLMSIACGLAWNIQIMIFF
                     RALQGAAGASMIPLVFTTAFIYYQGKELGLAAAVVSALASLSPTLGPTLGGWITDNLD
                     WRWL"
     gene            complement(490..1518)
                     /locus_tag="NBI74_004026"
     CDS             complement(490..1518)
                     /locus_tag="NBI74_004026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HCD1934281.1"
                     /translation="MIISKKQLIGVVAIGILLAGVVFFIWWVSKGRFIQTTDDAYIGG
                     NITTVTSKVSGYISAIEVRDNQSVKKGDIILRLDDRDYRANVARLEAKIKSSKANLES
                     IQATIAMQQSIIQSASETWQAVKHEEQKRLRDTERYEKLAQSAAISQQIIDNARFDYQ
                     QVAAKERKAANDFLVEKQRLAVLSAQEENVRASIEEVQAALTQALLDLEYTLVRAPID
                     GIVANRSAHTGSWVEGGTSLVSLVPVSELWVDANYKENQIAGMKPGMKAEIRADILKG
                     EVFHGHIESLSPATGASFSLIPIENATGNFTKIVQRVPVRIAFDDAKELKQLLRPGLS
                     VTVSVDER"
     gene            1615..2430
                     /locus_tag="NBI74_004027"
     CDS             1615..2430
                     /locus_tag="NBI74_004027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707689.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCD1934282.1"
                     /translation="MTITLQPKASPGHHIIGLDSEHLNGGTVPWHKHLYAQLLYPAEG
                     VVRVWAGESVWLVHASSALWLPPQMPHKFVATGNVLLKTVLVSEAESETLGKVCFMTG
                     ISPLLRELLIAINQLPPSQSTTDKQQLRFSALETLILQEIKMGVKMSLELPWPNDERL
                     QQLCENLLNNQGYLPTLDNLADKINVSSRTLMRLFVKETGLTFRHWVQQMHVISAVTL
                     LDDGYSLTKIAHRLGYASAESFGNMFKRRTGYSPGKFTRRLTMHDYAITRQMI"
     gene            2819..2962
                     /locus_tag="NBI74_004028"
                     /pseudo
     CDS             2819..2962
                     /locus_tag="NBI74_004028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415852.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
     gene            2973..3488
                     /gene="ogt"
                     /locus_tag="NBI74_004029"
     CDS             2973..3488
                     /gene="ogt"
                     /locus_tag="NBI74_004029"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HCD1934283.1"
                     /translation="MLRLLEEKIATPLGPLWVICDEQFRLRAVEWEEYSERMVQLLDI
                     HYRKEGYERISATNPGGLSDKLREYFAGNLSIIDTLPTATGGTPFQREVWKTLRTIPC
                     GQVMHYGQLAEQLGRPGAARAVGAANGSNPISIVVPCHRVIGRNGTMTGYAGGVQRKE
                     WLLRHEGYLLL"
     gene            3683..4435
                     /gene="fnr"
                     /locus_tag="NBI74_004030"
     CDS             3683..4435
                     /gene="fnr"
                     /locus_tag="NBI74_004030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007849744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate/nitrate reduction transcriptional
                     regulator Fnr"
                     /protein_id="HCD1934284.1"
                     /translation="MIPEKRIIRRIQSGGCAIHCQDCSISQLCIPFTLNEHELDQLDN
                     IIERKKPIQKGQTLFKAGDELKSLYAIRSGTIKSYTITEQGDEQITGFHLAGDLVGFD
                     AIGSGHHPSFAQALETSMVCEIPFETLDDLSGKMPNLRQQMMRLMSGEIKGDQDMILL
                     LSKKNAEERLAAFIYNLSRRFAQRGFSPREFRLTMTRGDIGNYLGLTVETISRLLGRF
                     QKSGMLAVKGKYITIENNDALAQLAGHTRNVA"
     gene            4587..5537
                     /gene="uspE"
                     /locus_tag="NBI74_004031"
     CDS             4587..5537
                     /gene="uspE"
                     /locus_tag="NBI74_004031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspE"
                     /protein_id="HCD1934285.1"
                     /translation="MAMYQNMLVVIDPNQDDQPALRRAVYLHQRIGGKIKAFLPIYDF
                     SYEMTTLLSPDERTAMRQGVISQRTDWIHEQAKYYLNAGVPIEIKVVWHNRPFEAIIQ
                     EVISGGHDLVLKMAHQHDRLEAVIFTPTDWHLLRKCPSPVWMVKDQPWPEGGKALVAV
                     NLASEEPYHNALNEKLVKETIELAEQVNHTEVHLVGAYPVTPINIAIELPEFDPSVYN
                     DAIRGQHLLAMKALRQKFGINENMTHVEKGLPEEVIPDLAEHLQAGIVVLGTVGRTGI
                     SAAFLGNTAEQVIDHLRCDLLVIKPDQYQTPVELDDEEDD"
     gene            complement(5587..5844)
                     /locus_tag="NBI74_004032"
     CDS             complement(5587..5844)
                     /locus_tag="NBI74_004032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2534 family protein"
                     /protein_id="HCD1934286.1"
                     /translation="MIMAKLKSAKGKKFLFGLLAVFIIAASVVTRATIGGVIEQYNIP
                     LSEWTTSMYVIQSSMIFVYSLVFTVLLAIPLGIYFLGGEEQ"
     gene            6088..7119
                     /gene="ynaI"
                     /locus_tag="NBI74_004033"
     CDS             6088..7119
                     /gene="ynaI"
                     /locus_tag="NBI74_004033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low conductance mechanosensitive channel YnaI"
                     /protein_id="HCD1934287.1"
                     /translation="MIAELFTNNALNLVIIFGSCAALILMSFWFRRGNRKRKGFLFHA
                     VQFLIYTIIISAVGSIINYVIENYKLKFITPGVIDFICTSLIAVILTIKLFLLINQFE
                     KQQIKKGRDITSARIMSRIIKITIIVVLVLLYGEHFGMSLSGLLTFGGIGGLAVGMAG
                     KDILSNFFSGIMLYFDRPFSIGDWIRSPDRNIEGTVAEIGWRITKIKTFDNRPLYVPN
                     SLFSSISVENPGRMTNRRITTTIGLRYEDAAKVGVIVEAVREMLKNHPAIDQRQTLLV
                     YFNQFADSSLNIMVYCFTKTTVWAEWLAAQQDVYLKIIDIVQSHGADFAFPSQTLYMD
                     NITPADQGR"
     gene            complement(7170..8783)
                     /gene="mppA"
                     /locus_tag="NBI74_004034"
     CDS             complement(7170..8783)
                     /gene="mppA"
                     /locus_tag="NBI74_004034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide ABC transporter
                     substrate-binding protein MppA"
                     /protein_id="HCD1934288.1"
                     /translation="MKHSVSVTCCALLVSSISLSYAAEVPSGTVLAEKQELVRHIKDE
                     PASLDPAKAVGLPEIQVIRDLFEGLVNQNEKGEIVPGVATQWKSNDNRIWTFTLRDNA
                     KWADGTPVTAQDFVYSWQRLVDPKTLSPFAWFAALAGINNAQAIIDGKATPDQLGVTA
                     VDAHTLKIQLDKPLPWFVNLTANFAFFPVQKANVESSKEWTKPGNLIGNGAYVLKERV
                     VNEKLVVVPNTHYWDNAKTVLQKVTFLPINQESAATKRYLAGDIDITESFPKNMYQKL
                     LKDIPGQVYTPPQLGTYYYAFNTQKGPTADQRVRLALSMTIDRRLMTEKVLGTGEKPA
                     WHFTPDVTAGFTPEPSPFEQMSQEELNAQAKTLLSAAGYGPQKPLKLTLLYNTSENHQ
                     KIAIAVASMWKKNLGVDVKLQNQEWKTYIDSRNTGNFDVIRASWVGDYNEPSTFLTLL
                     TSTHSGNISRFNNPAYDKVLAQASTENTVKARNADYNAAEKILMEQAPIAPIYQYTNG
                     RLIKPWLKGYPINNPEDVAYSRTMYIVKH"
BASE COUNT         2347 a         2127 c         2000 g         2398 t
ORIGIN      
        1 atagccatcg ccagtcgagg ttatcagtga tccagccccc cagagttggc cctagcgtcg
       61 gtgacagtga ggccagcgca ctgactaccg cagcagccaa ccccaactct ttaccctggt
      121 aataaataaa cgccgtcgtg aaaactaaag ggatcatgga tgcgccagca gctccctgta
      181 aagcacgaaa aaaaatcatg atctgaatat tccaagcaag tccacacgcg atactcatta
      241 aagtaaatat tcccgcggat agagtaaaaa gccaacgagt ggaaaatacc cgtgacagcc
      301 acccagagag cggaataaca ataatctcag caattaaata gctggtctga agccaggcca
      361 tttcgtcttt acctgcggac agaccaccac ctatttcatt tagggatgac gatacaattt
      421 gaatatcaag ctgagcaata aataatccca tacacatact ggcgaaagca atatgtttac
      481 gcatagacat catctctcat caacactgac ggtgacggac agtcccgggc gaagaagttg
      541 ctttaattcc tttgcgtcgt caaatgcaat ccgtaccggg acacgctgaa caattttggt
      601 gaaattgcca gtggcatttt ctatcggaat caagctgaaa ctggctcctg tcgctgggga
      661 aaggctttca atgtgaccgt gaaacacttc ccctttgaga atatcagcac ggatctcagc
      721 cttcattcct ggcttcattc ccgctatttg gttttcttta taatttgcat caacccacaa
      781 ctcactgact ggtactagtg aaactaacga tgtgccgcct tccacccaac tgcctgtatg
      841 ggcgctgcgg tttgcgacaa tgccatcaat cggcgcacga acaagggtat attccagatc
      901 cagtagtgcc tgagtcaaag cggcctgaac ttcttcgata cttgcacgta cgttttcttc
      961 ctgagcagat aaaactgcca gccgttgctt ctccaccagg aaatcgttcg cggctttgcg
     1021 ctcctttgct gcgacctgct ggtagtcaaa cctggcgtta tcaataattt gttgcgatat
     1081 tgccgctgat tgtgcgagtt tttcataacg ttcagtatct cgcaggcgct tttgctcctc
     1141 atgttttact gcctgccatg tttcactggc actttgaatt atcgactgct gcatcgcaat
     1201 agtggcttga atgctttcga gattggcttt gctacttttt atttttgcct caagcctggc
     1261 gacatttgca cgataatccc gatcatcgag acgcaggata atatcgcctt tttttacact
     1321 ctgattatcg cgcacctcta tagcagaaat ataaccagaa actttacttg tcaccgtggt
     1381 gatattacca ccaatgtaag catcatccgt cgtctgtata aatcttcctt tgcttaccca
     1441 ccagataaaa aagacaacgc cagccaaaag aataccgatc gctaccaccc caataagctg
     1501 ttttttagaa attatcatca ctctttacac cgtatatatc cggaaacttg catacgtcat
     1561 atttaaaatt taacatggct tatttgatga tttctgacaa aatacctact gtaaatgaca
     1621 atcacactgc aacccaaagc atcaccaggg catcacatta tcggactgga ttcggaacat
     1681 ctgaatggag gtacagttcc ctggcataaa catctctatg cccagttgct ttacccggct
     1741 gaaggagtcg ttcgtgtatg ggctggagaa agcgtctggc tagtgcatgc cagcagcgcg
     1801 ctttggctgc ccccacaaat gccgcataag tttgttgcga caggcaacgt acttttaaaa
     1861 accgtactgg tttcagaagc ggaaagtgaa acgctgggaa aagtttgctt catgacgggt
     1921 atttcacccc tgttaaggga attactaatt gcaattaacc aacttccacc gtcgcaaagt
     1981 accacagata aacaacagtt acgtttttca gccctcgaga cactgatact tcaggaaata
     2041 aaaatggggg tgaaaatgtc acttgaactc ccttggccaa atgatgagcg gctacagcag
     2101 ctttgtgaga atttgctaaa caaccaaggg tatctgccaa cactggataa tctagcggat
     2161 aaaattaacg tcagtagccg tacactcatg cgcttatttg ttaaagagac gggattaaca
     2221 tttcgccact gggttcagca aatgcacgtt atttctgcag tgacgcttct tgatgatgga
     2281 tattcgttga ccaaaattgc ccatcgtctg ggctatgcct cagctgaatc gttcggcaat
     2341 atgtttaagc gtcgaacggg ctactcgccc ggtaaattta ctcgtcgatt aacgatgcat
     2401 gattatgcaa tcacaagaca aatgatttaa cttatatttt cgtgaaatct gtcactgaag
     2461 aaaattggca acggatgggt aaaaccgtta taacactgtc accggtcacc aggaccccag
     2521 gccggattta agacgaggat gcactgctgt gtgtactgta gagtctggcg gatgtcgaca
     2581 ggctctattt ttttattcat tttttagccc tgtaaataac cacattctcg ccgggattgg
     2641 ggaatgagtg acgagatcag tggtgtctgg cggctatcac gccacagatc aaataacaga
     2701 taacctacgg taaaatttcg ctgactggaa ttatcccacc ctcgatccat gtgatggctt
     2761 gccctcattt cttcaggcag aaaaatcaaa aaatcgctta atactatcag actgcgacaa
     2821 accagacgcg aaactgggta cttactattc gttagtctta ccctatccac ttatcttttt
     2881 ggtggtatgg ctgctgatgt tgctggcgtg gtatcttgtt ggcttgccga taggtccggg
     2941 tatttaccca cgtttgtctt aagagagaac ggatgctgag attacttgaa gaaaaaattg
     3001 ccacgccact gggtccactg tgggtgattt gcgatgagca atttcgcctg cgggcggttg
     3061 aatgggaaga gtacagcgaa cgcatggtgc agctgctgga catccattat cgcaaagaag
     3121 gctatgagcg catttctgcc accaacccag gtggtttaag cgacaagctg cgtgaatatt
     3181 ttgccggtaa tcttagcatt attgatacgc ttcccactgc cacggggggg acgccatttc
     3241 agcgcgaagt ctggaaaaca ctgcgcacta tcccctgcgg gcaggtaatg cattatggcc
     3301 aactggctga acaattaggc cgccctggcg cggcgcgtgc cgttggtgcg gcaaacggat
     3361 cgaatcccat cagcatcgtc gtaccttgcc atcgggttat tggccgaaac ggcaccatga
     3421 ccggatatgc aggcggagtt cagcgaaaag agtggttatt gcgccacgaa ggttatcttt
     3481 tgctgtaaac attaaacaat ttgtgccagc ttgttcacac ttttatgtaa agtcaccctt
     3541 aacaacttaa gggttttcaa atagatagac atatatttac atctaatatc ggaattctct
     3601 gctgttaagg tttgcttaga cttacttgct ccctaaaaag atgttaaaat tgacaaatat
     3661 caattacggc ttgagcagac ctatgatccc ggaaaagcga attatacggc gcattcagtc
     3721 tggcggttgt gctatccatt gccaggattg cagcatcagc cagctttgca tcccgttcac
     3781 actcaacgaa catgagcttg atcagcttga taatatcatt gagcggaaga agcctattca
     3841 gaaaggccag acgctgttta aggctggaga tgaacttaaa tcgctttatg ccatccgctc
     3901 cggtacgatt aaaagttata ccatcactga gcaaggcgac gagcaaatca ctggtttcca
     3961 tttagcaggc gatctggtgg gatttgatgc catcggcagc ggtcatcacc cgagtttcgc
     4021 gcaggcgctg gaaacctcga tggtatgtga aatcccgttc gaaacgctgg acgatttgtc
     4081 tggtaaaatg ccgaatctgc gtcagcagat gatgcgtctg atgagcggtg aaatcaaagg
     4141 cgatcaggac atgatcctgc tgttgtcgaa gaaaaatgcc gaggaacgtc tggctgcatt
     4201 catctacaac ctgtcccgtc gttttgccca acgcggcttc tcccctcgtg aattccgcct
     4261 gacgatgact cgtggcgata tcggtaacta tctgggcctg acggtagaaa ccatcagccg
     4321 tctgctgggt cgcttccaga aaagcggcat gctggcagtc aaaggtaaat acatcaccat
     4381 cgaaaataac gatgcgctgg cccagcttgc tggtcatacg cgtaacgttg cctgattctt
     4441 ccgcataact cactatcctt ctgtcatatc attaaatttt tctgatttat tgatctgaca
     4501 gaaggttcat cactgtttca ttcaccagat atgggttaat cttttaatta caaactgcgt
     4561 tgacagttgt tgtaaggaga ccctgtatgg ctatgtatca gaacatgctc gttgttatcg
     4621 atcctaacca ggacgaccaa ccagcattgc ggcgagctgt ttatttacat caacggattg
     4681 gtggcaaaat taaagccttt ttgccgatct atgacttctc atacgaaatg accaccctgc
     4741 tctctccgga cgaacgtacc gctatgcgtc agggcgtcat cagccagcgt acagactgga
     4801 tccacgagca ggcaaaatat tatctcaatg ctggcgttcc cattgaaatt aaagtggtct
     4861 ggcataaccg tcctttcgaa gccatcattc aggaagtgat cagcggcggg cacgatttgg
     4921 tgctaaaaat ggcgcaccaa catgaccgtc tggaagcggt gatttttacg ccaacggact
     4981 ggcatctgtt acgcaaatgc ccaagcccgg tgtggatggt gaaagaccag ccgtggccgg
     5041 aagggggtaa ggcgctggtg gcggtgaatc tcgccagtga agagccgtac cataatgcgc
     5101 tcaatgaaaa actggtcaaa gagacgatcg aactggcaga acaagtcaac cataccgaag
     5161 ttcatctggt tggcgcttat ccggtaacgc caattaatat cgcgattgaa ctgccggaat
     5221 ttgacccgag cgtttacaac gatgccattc gtgggcaaca tttgctggca atgaaagctc
     5281 tgcggcagaa attcggcatt aatgaaaaca tgacgcacgt agaaaaaggt ctgcccgaag
     5341 aggtgattcc tgatttggcg gagcacttac aggcggggat tgtggttctg ggcacggtcg
     5401 gacgcaccgg tatttcagca gcattcctcg gcaacacggc ggaacaggtg attgatcatc
     5461 ttcgctgcga cctgctggtc attaaacctg accagtatca gactcccgtt gaactggatg
     5521 acgaagaaga cgattaacga ctcttcaata actgacaagg cccgagttat cgggcctatt
     5581 ttttacttac tgctcttcgc cgccaaggaa ataaattccc aacgggattg ccagcaacac
     5641 agtaaagacc aggctataaa caaaaatcat cgatgactga atcacataca ttgatgtcgt
     5701 ccactcagac agcggaatat tgtactgttc tataacgccg ccgatggtcg cgcgagtcac
     5761 aaccgacgcc gcaataatga aaaccgccaa caaaccaaag agaaatttct tccctttcgc
     5821 tgacttcagt ttcgccataa tcatgatgta atccttgtgc agacaaattt tttgttacct
     5881 aaacgtaaca ataccatgac atttcaccgt tgtctgctct gaaaatctca cctcttagct
     5941 ggatttacaa tcaacgcatt gattagttag ctaatgttgc tacttccttc cctgactatt
     6001 gatgatctga atgaattgtc gcggtaaaat agtcccgcca ggggtttccg gcatgacgac
     6061 aattatctgg aggagcctga taaaacaatg atcgctgaac tgtttacaaa taatgcgctt
     6121 aatctggtca ttattttcgg tagctgcgca gcattgattc tgatgagctt ttggtttcgc
     6181 cgtggaaatc gtaaaagaaa aggattttta ttccatgcgg tacaattttt aatctacacc
     6241 ataattatta gtgctgttgg tagcatcatt aactatgtta tagaaaacta taaactcaaa
     6301 tttatcaccc caggcgttat cgattttatc tgtacgtccc tgattgcggt tattctgacg
     6361 attaagctat ttctgctgat taatcagttt gaaaaacagc agattaaaaa aggtcgcgat
     6421 atcaccagtg cgcggatcat gtcgcgcatt atcaaaatca ccattattgt ggtgcttgtt
     6481 ctgctttatg gcgaacattt cggcatgagc ctttctggct tgttgacctt tggtggtatt
     6541 ggtggtctgg ctgtcggtat ggccggtaaa gatattctga gtaacttctt ttccgggatt
     6601 atgctctatt tcgaccgtcc tttcagtatt ggcgactgga tccgttcacc ggacagaaat
     6661 atcgaaggta cagtagcgga aattggctgg cgaattacca aaattaagac ctttgataat
     6721 cgtccattgt acgtaccgaa ctcgctgttt tcgtcgatca gcgtagaaaa tcctggacga
     6781 atgaccaacc ggcgcattac cacgaccatt ggtttacgtt atgaggatgc ggcaaaagtg
     6841 ggcgttattg tcgaagccgt acgtgagatg ctgaaaaatc acccggccat cgaccagcga
     6901 caaaccttac tggtttattt caaccagttt gctgattcgt cattaaatat tatggtttat
     6961 tgctttacca aaaccacggt atgggctgaa tggcttgctg cacagcaaga cgtttatttg
     7021 aagattatcg atattgtaca gtcacacggc gcggactttg ccttccccag ccagacgctg
     7081 tatatggata acattacacc ggcggaccag ggtcgctaaa tcagacatca ctggcgttat
     7141 ccacaagggt aacgccagtt ctcatcacat caatgcttca caatatacat agtccgactg
     7201 tacgccacat cttcagggtt attaatggga taacctttca gccacggctt gattaatcgt
     7261 ccattggtat attgataaat tggtgcaatc ggtgcttgct ccatgaggat tttttctgcc
     7321 gcgttgtaat cggcattacg cgctttaacg gtattttccg tcgatgcctg ggccagaact
     7381 ttgtcatatg ccggattgtt aaagcgtgaa atattcccgg aatgcgttga cgttaataat
     7441 gtcaggaaag tggagggttc attataatcc cctacccacg aggcgcggat aacatcaaaa
     7501 ttgccggtat tacggctatc gatataggtt ttccattcct ggttttgcaa tttaacatct
     7561 acgcccaggt tctttttcca catcgatgct acagcaatcg caattttttg atggttttct
     7621 gaagtgttat acaaaagcgt cagcttcaac ggtttttgcg gaccataacc agcagcgctc
     7681 aacaaagttt ttgcctgcgc attcagttct tcctgactca tttgttcaaa cggcgaaggt
     7741 tccggcgtaa atcccgcggt aacatctggt gtaaaatgcc acgctggctt ttcgcccgtc
     7801 cccaacactt tttcggtcat caggcggcga tctatcgtca tacttaatgc caggcgaacg
     7861 cgttgatctg ccgtcggccc tttttgcgtg ttaaacgcat aataataggt cccgagctgc
     7921 ggcggcgtat aaacctgccc cggaatatcc ttcaacagct tctgatacat atttttcggg
     7981 aaggattcgg tgatatcaat atcccccgcg agataacgct tagtggctgc ggattcctga
     8041 ttaattggca ggaaggtcac tttttgcagt accgttttgg cgttatccca ataatgggta
     8101 ttcggcacta cgaccagttt ttcattgact acgcgctctt taagaacata agcgccattg
     8161 ccgatcagat ttccgggttt cgtccactct ttactgcttt ctacgttggc tttttgcacc
     8221 gggaagaagg caaagttagc ggttaaattc acaaaccacg gcaacggttt atcaagctgg
     8281 attttcaaag tatgggcatc aactgcggta acgccaagct ggtcaggcgt ggctttacca
     8341 tcaataatcg cctgtgcgtt gttgattccc gccagcgcgg caaaccatgc aaatggcgaa
     8401 agcgttttcg ggtccaccag acgttgccag ctgtagacaa aatcttgcgc cgttaccggt
     8461 gtgccatccg cccattttgc gttatcgcgc agggtaaaag tccagatacg gttgtcatta
     8521 cttttccact gagtcgcaac gccggggaca atctcccctt tctcgttctg attcaccagt
     8581 ccttcaaaca gatcgcgaat gacctgaatc tccggcaggc ccacggcttt agcgggatcc
     8641 agcgacgctg gctcatcttt aatgtggcgc accagctcct gcttctctgc cagtactgtg
     8701 ccgctcggaa cttcagcagc atacgaaaga gaaatgctgc tgaccaacag cgcacaacac
     8761 gtgactgaaa cagagtgctt cataagtttc cctctaaccg acattaaaat tgatgcgtaa
     8821 ttatttgttt cgccttaaag aaatgcaaac aacttccatc aaaaaagtga ca
//
LOCUS       DAHHTG010000163         8795 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.166, whole genome shotgun sequence.
ACCESSION   DAHHTG010000163 DAHHTG010000000
VERSION     DAHHTG010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8795)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8795)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8795
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.166"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            complement(33..659)
                     /gene="upp"
                     /locus_tag="NBI74_004035"
     CDS             complement(33..659)
                     /gene="upp"
                     /locus_tag="NBI74_004035"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HCD1934289.1"
                     /translation="MKIVEVKHPLVKHKLGLMREQDISTKRFRELASEVGSLLTYEAT
                     ADLETEKVTIEGWNGPVEIDQIKGKKITVVPILRAGLGMMDGVLENVPSARISVVGMY
                     RNEETLEPVPYFQKLVSNIDERMALIVDPMLATGGSVIATIDLLKKAGCSSIKVLVLV
                     AAPEGIAALEKAHPDVELYTASIDQGLNEHGYIIPGLGDAGDKIFGTK"
     gene            984..2021
                     /gene="purM"
                     /locus_tag="NBI74_004036"
     CDS             984..2021
                     /gene="purM"
                     /locus_tag="NBI74_004036"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HCD1934290.1"
                     /translation="MTDKTSLSYKDAGVDIDAGNALVGRIKGVVKKTRRPEVMGGLGG
                     FGALCALPQKYREPVLVSGTDGVGTKLRLAMDLKRHDTIGIDLVAMCVNDLVVQGAEP
                     LFFLDYYATGKLDVDTASAVISGIAEGCLQSGCSLVGGETAEMPGMYHGEDYDVAGFC
                     VGVVEKSEIIDGSKVSDGDVLIALGSSGPHSNGYSLVRKILEVSGCDPQTTELDGKPL
                     ADHLLAPTRIYVKSVLKLIEKVDVHAIAHLTGGGFWENIPRVLPDNTQAVIDESSWQW
                     PEVFNWLQTAGNVERHEMYRTFNCGVGMIIALPAPEVDKALALLNANGENAWKIGIIK
                     ASDSEQRVVIE"
     gene            2021..2659
                     /gene="purN"
                     /locus_tag="NBI74_004037"
     CDS             2021..2659
                     /gene="purN"
                     /locus_tag="NBI74_004037"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HCD1934291.1"
                     /translation="MNIVVLISGNGSNLQAIIDACKTNKIKGTVRAVFSNKADAFGLE
                     RARQAGIATHTLITSAFDSREAYDRELIHEIDMYAPDVVVLAGFMRILSPAFVSHYAG
                     RLLNIHPSLLPKYPGLHTHRQALENGDEEHGTSVHFVTDELDGGPVILQAKVPVFAGD
                     TEDDITARVQTQEHAIYPLVISWFADGRLKMHENAAWLDGQRLPPQGYAADE"
     gene            2831..4897
                     /gene="ppk1"
                     /locus_tag="NBI74_004038"
     CDS             2831..4897
                     /gene="ppk1"
                     /locus_tag="NBI74_004038"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HCD1934292.1"
                     /translation="MGQEKLYIEKELSWLSFNERVLQEAADKSNPLIERMRFLGIYSN
                     NLDEFYKVRFAELKRRIIISEEQGSNSHSRHLLGKIQSRVLKADQEFDGLYNELLLEM
                     ARNQIFLINERQLSVNQQNWLRHYFKQYLRQHITPILINPDTDLVQFLKDDYTYLAVE
                     IIRGDTIRYALLEIPSDKVPRFVNLPPEAPRRRKPMILLDNILRYCLDDIFKGFFDYD
                     ALNAYSMKMTRDAEYDLVHEMEASLMELMSSSLKQRLTAEPVRFVYQRDMPNALVEVL
                     REKLTISRYDSIVPGGRYHNFKDFINFPNVGKANLVNKPLPRLRHIWFDKAQFRNGFD
                     AIRERDVLLYYPYHTFEHVLELLRQASFDPSVLAIKINIYRVAKDSRIIDSMIHAAHN
                     GKKVTVVVELQARFDEEANIHWAKRLTEAGVHVIFSAPGLKIHAKLFLISRKENGEVV
                     RYAHIGTGNFNEKTARLYTDYSLLTADARITNEVRRVFNFIENPYRPVTFDYLMVSPQ
                     NSRRLLYEMVDREIANAQQGLPSGITLKLNNLVDKGLVDRLYAASSSGVPVNLLVRGM
                     CSLIPNLEGISDNIRAISIVDRYLEHDRVYIFENGGDKKVYLSSADWMTRNIDYRIEV
                     ATPLLDPRLKQRVLDIIDILFSDTVKARYIDKELSNRYVPRGNRRKVRAQLAIYDYIK
                     SLEQSE"
     gene            4902..6443
                     /gene="ppx"
                     /locus_tag="NBI74_004039"
     CDS             4902..6443
                     /gene="ppx"
                     /locus_tag="NBI74_004039"
                     /EC_number="3.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HCD1934293.1"
                     /translation="MPIHDKSPRPQEFAAVDLGSNSFHMVIARVVDGAMQIIGRLKQR
                     VHLADGLGPDNMLSEEAMTRGLNCLSLFAERLQGFSPASVCIVGTHTLRQALNATDFL
                     KRAEKVIPYPIEIISGNEEARLIFMGVEHTQPEKGRKLVIDIGGGSTELVIGENFEPI
                     LVESRRMGCVSFAQLYFPGGVINKENFQRARMAAAQKLETLTWQFRIQGWNVAMGASG
                     TIKAAHEVLMEMGEKDGIITPERLEKLVKEVLRHRNFASLSLPGLSEERKTVFVPGLA
                     ILCGVFDALAIRELRLSDGALREGVLYEMEGRFRHQDVRSRTASSLANQYHIDSEQAR
                     RVLDTTMQMYEQWREQQPKLAHPQLEALLRWAAMLHEVGLNINHSGLHRHSAYILQNS
                     DLPGFNQEQQLMMATLVRYHRKAIKLDDLPRFTLFKKKQFLPLIQLLRLGVLLNNQRQ
                     ATTTPPTLTLITDDSHWTLRFPHDWFSQNALVLLDLEKEQEYWEGVAGWRLKIEEEST
                     PEIAA"
     gene            complement(6482..8725)
                     /gene="pdeF"
                     /locus_tag="NBI74_004040"
     CDS             complement(6482..8725)
                     /gene="pdeF"
                     /locus_tag="NBI74_004040"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeF"
                     /protein_id="HCD1934294.1"
                     /translation="MKLNATYIKIRDKWWGLPLFLPSLILPIFAHINTFAHISSGEVF
                     LFYLPLALMISMMMFFSWAALPGIALGIFVRKYAELGFYETLSLTANFIIIIILCWGG
                     YRVFTPRRNNVSHGDSRLISQRLFWQIVFPATLFLILFQFAAFVGLLASREYLVGVMP
                     FNLGTLINYQALLVGNLIGVPLCYFIIRVVRNPFYLRSYYSQLKQQVDAKVTKKEFAI
                     WLLALGALLLLLCMPLNEKSTIFSTNYTLSLLLPLMMWGAMRYGYKLISLLWAVVLMI
                     SIHSYQNYIPIYPGYTTQLTITSSSYLVFSFIVNYMAVLATRQRAVVRRIQRLAYVDP
                     VVHLPNVRALNRALRDAPWSALCYLHIPGMEMLVKNYGIMLRIQYKQKLSHWLSPLLE
                     PGEDVYQLSGNDLALRLNTESHQERITALDSHLKQFRFFWDGMPMQPQIGVSYCYVRS
                     PVNHIYLLLGELNTVAELSIVTNVPENMQRRGAMYLQRELKDKVAMMNRLQQALEHNH
                     FFLMAQPITGMRGDVYHEILLRMKGENDELISPDSFLPVAHEFGLSSSIDMWVIEHTL
                     QFMAENRAKMPAHRFAINLSPTSVCQARFPVEVSQLLAKYQIEAWQLIFEVTESNALT
                     NVKQAQITLQHLQELGCQIAIDDFGTGYASYARLKNVNADLLKIDGSFIRNIVSNSLD
                     YQIVASICHLARMKKMRVVAEYVENEEIREAVLSLGIDYMQGYLIGKPQPLIDTLNEI
                     EPIRESA"
BASE COUNT         2262 a         2171 c         2185 g         2177 t
ORIGIN      
        1 ctcttaaagt cggctttaat tatttttatt ctttatttcg taccaaagat tttgtcaccg
       61 gcatcgccga ggcccggaat aatgtatccg tgctcgttca gtccctggtc aatcgatgca
      121 gtatacagtt cgacgtccgg gtgcgctttt tccagcgcag cgataccttc tggtgcagct
      181 accagcacca gaactttgat gctgctgcag cccgcttttt tcagcaggtc gatggtcgcg
      241 ataacggaac caccggttgc cagcattggg tcaacgatca gcgccatacg ctcatcgatg
      301 ttagaaacca gtttctggaa gtacggtacc ggctccagcg tttcttcatt acggtacata
      361 ccgacgacgc tgatgcgcgc gctcggaacg ttttccagca caccgtccat catccccaga
      421 cccgcacgca gaattggcac aacggtaatt ttcttacctt tgatctggtc gatttctacc
      481 gggccgttcc agccttcgat agttactttt tccgtttcga ggtcggcggt cgcttcgtaa
      541 gtcagcaggc tacccacttc ggaagcgagt tcgcgaaagc gcttggtgct gatatcttgc
      601 tcacgcatca gtcccagctt gtgtttgacg agtgggtgtt tgacttccac gatcttcata
      661 ctctttctcc tttgaggggc agccacaaaa aaaatcgacg gattatacct cctttcttca
      721 aggcggcaat attattttcg ttgactttag tcaaaatgat aacggtttga gataaagtta
      781 ttttatattc aaatagttat gaaagaagat tattccatcc gaaaacgatc ctttgccctg
      841 gtacaagtat tctttcgccg cgcgcctggg gaaaagacgt gcaaaaaggt tgtgtaaagc
      901 agtctcgcaa acgtttgctt tccctgttag aattgcgccg aattttattt ttctaccgca
      961 agtaacgcgt ggggacccaa gcagtgaccg ataaaacctc tcttagctac aaagatgccg
     1021 gtgttgatat tgacgcgggt aatgctctgg ttggaagaat caaaggcgta gtgaagaaaa
     1081 cgcgtcgtcc ggaagtgatg ggcggtctgg gcggcttcgg tgcgctgtgt gcattgccgc
     1141 aaaaatatcg tgaacctgtg ctggtttccg gcactgacgg cgtaggtacc aagctgcgtc
     1201 tggcgatgga cttaaaacgt cacgacacca ttggtattga tctggtcgcc atgtgcgtta
     1261 atgacctggt ggtgcaaggt gcggaaccgc tgtttttcct cgactattac gcaaccggaa
     1321 aactggatgt tgataccgct tcagcggtga tcagcggcat tgcggaaggt tgtctgcaat
     1381 cgggctgttc actggtgggt ggcgaaacgg cggaaatgcc ggggatgtat cacggtgaag
     1441 attacgatgt cgctggtttc tgcgttggcg tggtagaaaa atcagaaatc atcgacggat
     1501 ctaaagtcag cgacggcgat gtgctgattg cactcggctc cagcggcccg cactcgaacg
     1561 gctattcgct ggtgcgcaaa attcttgaag tcagcggttg tgatccgcaa accaccgaac
     1621 ttgatggtaa gccattagcc gatcatctgc tggcaccgac ccgcatttac gtgaagtcag
     1681 tgctgaaatt gattgaaaag gtcgatgtgc atgccattgc gcacctgacc ggcggcggct
     1741 tctgggaaaa cattccgcgc gtattgccag ataataccca ggcagtgatt gatgaatctt
     1801 cctggcagtg gccggaagtg ttcaactggc tgcaaacggc aggtaacgtt gagcgccatg
     1861 aaatgtatcg caccttcaac tgcggcgtcg gtatgattat tgccctgcct gctccggaag
     1921 tggacaaagc cctcgccctg ctcaatgcca acggtgaaaa cgcgtggaaa atcggtatca
     1981 tcaaagcctc tgattccgaa caacgcgtgg ttatcgaata atgaatattg tggtgcttat
     2041 ttccggcaac ggaagtaatt tgcaggcaat tattgacgcc tgcaaaacca acaaaattaa
     2101 aggcaccgta cgggcagttt tcagcaataa ggccgacgcg ttcggccttg aacgcgcccg
     2161 ccaggcgggt attgcaacgc atacgctcat caccagcgcg tttgacagtc gtgaagccta
     2221 tgaccgggag ttgattcatg aaatcgacat gtacgcaccc gatgtggtcg tgctggctgg
     2281 ttttatgcgc attctcagcc cggcgtttgt ctcccactat gctgggcgtt tgctgaacat
     2341 tcacccttct ctgctgccga aatatcccgg attacacacc catcgtcagg cgctggaaaa
     2401 tggcgatgaa gagcacggta catcggtgca tttcgtcacc gatgaactgg acggtggccc
     2461 ggttatttta caggcgaaag tcccggtatt tgctggtgat acggaagatg acatcaccgc
     2521 ccgcgtgcaa acccaggaac acgctattta tccactggtg attagctggt ttgccgatgg
     2581 tcgtctgaaa atgcacgaaa acgccgcgtg gctggatggt caacgtctgc cgccgcaggg
     2641 ctacgctgcc gacgagtaat gcccccgtag ttaaagcgcc agctctgccg ctggcgtttt
     2701 tcaattcacc tgtaaatcgc aagctccagc agtttttttc ccccttttct ggcatagttg
     2761 gacatctgcc aatattgctc gccataatat ccaggcagtg tcccgtgaat aaaacggagt
     2821 aaaagtggta atgggtcagg aaaagctata catcgaaaaa gagctcagtt ggttatcgtt
     2881 caatgaacgc gtgcttcagg aagcggcgga caaatctaac ccgctgattg aaaggatgcg
     2941 tttcctgggg atctattcca ataaccttga tgagttctat aaagtccgct tcgctgaact
     3001 gaagcgacgc atcattatta gcgaagaaca aggttccaac tctcattccc gccatttact
     3061 gggcaaaatt cagtcccggg tgctgaaagc cgatcaggaa ttcgacggcc tctacaacga
     3121 gttgttgcta gagatggcgc gcaaccagat cttcctgatt aatgaacgcc agctctccgt
     3181 caatcaacaa aactggctgc gtcattattt taagcagtat ctgcgtcagc acattacgcc
     3241 gattttaatc aatcctgaca ctgatttagt gcagttcctg aaagatgatt acacctatct
     3301 ggcggtggaa attatccgtg gcgataccat ccgttacgcg ctgctggaga tcccatcaga
     3361 taaagtgccg cgctttgtga atttaccgcc agaagcgccg cgtcgacgca agccgatgat
     3421 tcttctggat aacattctgc gttactgcct tgatgatatt ttcaaaggct tctttgatta
     3481 tgacgcgctg aatgcctatt caatgaagat gacccgcgat gccgaatacg atttagtgca
     3541 tgagatggaa gccagcctga tggagttgat gtcttccagt ctcaagcagc gtttaactgc
     3601 tgagccggtg cgttttgttt atcagcgcga tatgcccaat gcgctggttg aagtgttacg
     3661 cgaaaaactg actatttccc gctacgactc catcgtcccc ggcggtcgtt atcataattt
     3721 taaagacttt attaatttcc ccaatgtcgg caaagccaat ctggtgaaca aaccactgcc
     3781 acgtttacgc cacatttggt ttgataaagc ccagttccgc aacggttttg atgccattcg
     3841 tgagcgcgat gtgctgctct attatcctta tcataccttt gagcatgtac tggaactgct
     3901 gcgtcaggct tcgttcgacc cgagcgtact ggcgattaaa attaacattt accgcgtggc
     3961 gaaagattca cgcatcatcg actcgatgat ccacgccgca cacaacggta agaaagtcac
     4021 cgtggtggtt gagttacagg cacgtttcga cgaagaagcc aacattcact gggcgaagcg
     4081 cctgaccgaa gcaggcgtgc acgttatctt ctctgcgccg gggctgaaaa ttcacgccaa
     4141 actgttcctg atttcacgta aagaaaacgg tgaagtggtg cgttacgcac acatcgggac
     4201 cgggaacttt aacgaaaaaa ccgcgcgtct ttatactgac tattcgttgc tgaccgccga
     4261 tgcgcgcatc actaacgaag tacggcgggt gttcaacttt attgaaaacc cataccgtcc
     4321 ggtgacattt gattatttaa tggtgtcgcc gcaaaactct cgccgtctgt tgtatgaaat
     4381 ggtggaccgc gagatcgcca acgcgcagca agggctgccc agtggtatca ccctgaagct
     4441 aaataacctt gtcgataaag gtctggttga tcgtctgtat gcggcctcca gctccggcgt
     4501 accggttaat ctgctggtgc gcggaatgtg ttctctgatc cccaatctgg aaggcattag
     4561 cgacaacatt cgtgccatca gtattgttga ccgttacctt gaacatgacc gggtttatat
     4621 ttttgaaaat ggcggcgata aaaaggtcta cctttcttcc gccgactgga tgacgcgcaa
     4681 tattgattat cgtattgaag tggcaacgcc gctgctcgat ccgcgcctga agcagcgggt
     4741 gctggacatc atcgacatat tgttcagcga tacggtcaaa gcacgttata tcgataaaga
     4801 actcagtaat cgctacgttc cccgcggcaa tcgccgcaaa gtacgggcgc agttggcgat
     4861 ttacgactac atcaaatcac tcgaacaatc tgaataaccc tatgccaata cacgataaat
     4921 cccctcgtcc gcaggagttt gctgcggtcg atcttggttc aaacagtttt cacatggtca
     4981 tagcccgtgt ggtagatggt gccatgcaga ttattggccg cctgaaacag cgggtgcatc
     5041 tggcggacgg cctggggcca gataatatgt tgagtgaaga ggcaatgacg cgcggtttga
     5101 actgtctgtc gctatttgcc gaaaggctac aagggttttc ccctgccagc gtctgtatag
     5161 ttggtaccca tacgctgcgt caggcgctga acgccactga ctttctgaaa cgcgcggaaa
     5221 aagtcattcc ctacccgatc gaaattattt ccggtaatga agaagcccgt ctgattttta
     5281 tgggcgtgga acatacccaa ccggaaaaag gtcgcaaact ggttattgat attggcggcg
     5341 gttctacaga actggtgatt ggtgaaaatt tcgaaccgat tctcgttgaa agccgccgga
     5401 tgggttgtgt cagctttgcc cagctttatt tccctggcgg ggtcatcaat aaagagaatt
     5461 ttcagcgcgc tcgcatggcg gcagcacaaa aactggaaac gttaacctgg caatttcgaa
     5521 ttcagggctg gaacgtggca atgggcgctt ccggtaccat aaaagccgcc catgaagtgt
     5581 taatggaaat gggcgagaaa gacgggataa ttaccccgga acgtctggaa aaactggtaa
     5641 aagaagtttt acgtcaccgt aatttcgcat cgctgagttt accgggtctt tccgaagaac
     5701 ggaaaacagt cttcgttccg ggactggcga ttttatgcgg tgtgtttgat gctttagcca
     5761 tccgtgaact gcgcctttct gacggggcgt tacgcgaagg cgtactgtat gaaatggaag
     5821 gacgtttccg tcatcaggat gtgcgtagtc gcactgccag cagcctcgcc aaccagtatc
     5881 acatcgacag cgaacaggcc cgacgggtgc tggataccac tatgcaaatg tacgaacagt
     5941 ggcgggaaca gcaaccgaag ctggcgcatc cacaactgga ggcgctactg cgatgggccg
     6001 ccatgctgca tgaggtcggg ttgaatatca accacagcgg tttgcatcgc cactccgctt
     6061 atattctgca aaacagcgac ttgccgggtt ttaatcagga acagcagctg atgatggcga
     6121 cactggtgcg ctatcaccgt aaagcgatta aactcgacga tctgccgcgc tttaccttgt
     6181 ttaagaagaa acagttcctg ccactgattc agctattgcg ccttggcgta ttactcaata
     6241 atcaacgtca ggcaaccacc acaccgccaa cattgacgct gattactgat gacagtcact
     6301 ggacactgcg tttcccgcat gactggttta gtcagaatgc gctggtactg cttgatctgg
     6361 aaaaggagca agaatactgg gaaggcgtgg ctggctggcg gttgaaaatt gaagaagaaa
     6421 gtacaccaga aatcgccgct taatgctttg cgggccgacg agaaatgtcg gcccgcatta
     6481 ttcaggcact ttcgcgaatg ggttcgattt cattcagcgt atcaattaac ggttgcggct
     6541 taccaataag ataaccctgc atataatcga tccccaaaga gagcaccgcc tcgcggatct
     6601 cttcgttttc aacgtactct gccactaccc gcattttctt cattcgcgcc aggtggcaaa
     6661 tcgacgccac tatctgataa tccagactat ttgacacaat attgcggata aaactgccgt
     6721 caattttaag cagatcggca ttcacatttt taagccgcgc atagctggcg tagccggtgc
     6781 cgaaatcatc aatcgcaatc tggcagccta attcctgaag atgctgcaag gtgatttgcg
     6841 cctgcttaac attggtcaga gcattacttt cggtgacttc aaaaataagt tgccacgctt
     6901 caatctgata tttagccagc agctgactga cttcaacagg aaaacgagcc tgacataccg
     6961 aggttggaga cagattaata gcaaaacggt gagcgggcat cttcgctctg ttttcagcca
     7021 taaattgcag cgtatgctca atgacccaca tgtcgatact cgacgataaa ccaaattcgt
     7081 gcgcgaccgg taagaagcta tcggggctga tcagctcatc attctcaccc ttcatgcgca
     7141 gaagaatttc atggtaaaca tcaccacgca taccggtaat cggctgggcc atcaggaaaa
     7201 aatggttgtg ttccagcgcc tgctgtagtc gattcatcat cgcgacttta tctttcaatt
     7261 cgcgttgcaa atacattgct ccgcgacgct gcatattttc cgggacgttg gtcacgatgg
     7321 aaagttcggc caccgtattt agctctccca gcagcaggta gatatgattc actggcgagc
     7381 gcacatagca gtaactgacg ccaatctgcg gttgcatcgg cattccatcc caaaagaaac
     7441 gaaattgctt gagatggcta tccagtgcgg taatgcgctc ctggtgcgat tctgtattca
     7501 gccgcagcgc gagatcgtta cccgaaagct gataaacatc ttcacccggt tccagcaatg
     7561 gtgacagcca gtgagaaagt ttttgcttgt attgaatccg cagcatgatg ccatagttct
     7621 taaccagcat ttccatgcca gggatgtgta aatagcatag cgcagaccag ggggcatcac
     7681 gcaacgcgcg attcagggcg cgaacatttg gcagatgaac caccgggtcc acatacgcaa
     7741 gccgctgaat gcgtctgact acagctcgct gacgggttgc cagtacagcc atataattga
     7801 caataaaaga gaataccaga taactggagg aggttatggt cagctgcgtg gtatagccag
     7861 gataaatggg aatgtaattt tgatagctat ggatgctgat catcaacacg accgcccaga
     7921 gcaatgaaat caacttataa ccatagcgca tcgctcccca catcatcagg ggcagcaata
     7981 atgacaaggt gtaattggtg ctaaaaatcg tgcttttttc atttaacggc atgcataaca
     8041 gcaacaataa ggcacctaat gccagtagcc agattgcgaa ctcttttttg gtaactttgg
     8101 catcaacctg ctgttttaat tgcgaataat agctacgtaa ataaaacgga tttcgcacca
     8161 cccgaatgat aaagtagcac agcgggacac caatcagatt acccaccagc aaggcctgat
     8221 aattgattaa ggtcccgagg ttaaagggca tgacgccgac cagatattct ctgctcgcca
     8281 gtaaccctac aaacgcagca aactggaaaa gtatcagaaa cagcgttgca ggaaacacaa
     8341 tctgccagaa tagtcgctgg gaaattaaac ggctatcacc atgtgaaacg ttgttacgcc
     8401 ggggggtaaa aaccctgtaa ccgccccaac agagaatgat aatgataata aaattagccg
     8461 ttaatgagag cgtttcgtaa aaacccagct ctgcatattt gcggacaaaa atccctaagg
     8521 cgatccctgg caatgccgcc cagctgaaaa acatcatcat gctgatcatc agtgccagtg
     8581 gcagataaaa gagaaaaact tcaccggaag aaatatgcgc gaaagtatta atgtgggcga
     8641 aaatgggcaa gattaaagaa ggcaggaaca gcggaagccc ccaccattta tcacgtattt
     8701 ttatataagt tgcattcagt ttcatagatg ctcagcagaa tcccccacat cctgaaggag
     8761 gtgtattcag acaggcatcc cacctgactt cgaat
//
LOCUS       DAHHTG010000164         8711 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.167, whole genome shotgun sequence.
ACCESSION   DAHHTG010000164 DAHHTG010000000
VERSION     DAHHTG010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8711)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8711)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8711
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.167"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            5..1723
                     /gene="iucA"
                     /locus_tag="NBI74_004041"
     CDS             5..1723
                     /gene="iucA"
                     /locus_tag="NBI74_004041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012005367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobactin synthase IucA"
                     /protein_id="HCD1934295.1"
                     /translation="MPSEKPATDVAAQCFLNALIRETTDWKLTEYPPDELLIPLDEQK
                     SLHFRVAYFSPTQHHRFAFPARLVTASGSYPVDFTTLSRLIIDKLRHQLFLPVPLCET
                     FHQRVLESHVHTQQAIDARHDWAALREKALNFGEAEQALLTGHAFHPAPKSHEPFNRR
                     EAERYLPDMAPHFPLRWFSVDKTQIAGESLHLNLQQRLTRFAAENAPQLLNELSDNQW
                     LFPLHPWQGEYLLQQGWCQALVAKGLIKDLGEAGTSWLPTTSSRSLYCATSRDMIKFS
                     LSVRLTNSIRTLSVKEVKRGMRLARLAQTDGWQMLQVRFPTFRVMQEDGWAGLRDLNG
                     NIMQESLFALRENLLVDQPKSQTNVLVSLTQAAPDGGDSLLVSAVKRLSDRLGITVQQ
                     AAHAWVDAYCQQVLKPLFTAEADYGLVLLAHQQNILVQMLGDLPVGFIYRDCQGSAFM
                     PHATDWLDSIGEAQAENIFTHEQLLRYFPYYLLVNSTFAVTAALGAAGLDSESNLMAR
                     VRASLAEVRDQVTHKTCLNYVLESPYWNVKGNFFCYLNDHNENTIVDPSVIYFDFANP
                     LQAQEV"
     gene            1724..2671
                     /gene="iucB"
                     /locus_tag="NBI74_004042"
     CDS             1724..2671
                     /gene="iucB"
                     /locus_tag="NBI74_004042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(6)-hydroxylysine O-acetyltransferase"
                     /protein_id="HCD1934296.1"
                     /translation="MSEANIIHSRYGLRCEKLDKPLNLGWGLDNSAVLHCPGELPTGW
                     LCDALDQIFIAAPQLSAVALPWAEWREEPQALTLFGQVKSDIIHRTAFWQLPLWLSSP
                     ANRASGEMVFDAEREIYFPQRPPRPQGEVYRRYDPRIRRMLSFRIADPVSDAERFTRW
                     MNDPRVEYFWEQSGSLEVQTAYLERQLTGKHAFPLIGCFDDRPFSYFEIYWAAEDRIG
                     RHYSWQPFDRGLHLLVGEQQWRGAHYVQSWLRGLTHYLLLDEPRTQRTVLEPRTDNLR
                     LFRHLEPAGYRTIKEFDFPHKRSRMVMADRHHFFTEVGL"
     gene            2671..4413
                     /gene="iucC"
                     /locus_tag="NBI74_004043"
     CDS             2671..4413
                     /gene="iucC"
                     /locus_tag="NBI74_004043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobactin synthase IucC"
                     /protein_id="HCD1934297.1"
                     /translation="MNHKDWDFVNRRLVAKMLSEMEYEQVFHAESQGDDHYCINLPGA
                     QWRFIAERGIWGWLWIDAQTLRCTDEPVLAQTLLMQLKPVLSMSDATVAEHMQDLYAT
                     LLGDLQLLKARRGLSASDLINLDADRLQCLLSGHPKFVFNKGRRGWGKEALERYAPEY
                     TNTFRLHWLAVKREHMIWRCDNDLDIQQLLTAAMDPQEFTRFSQVWQENGLDHNWLPL
                     PVHPWQWQQKIATDFIADFAEGRMVSLGEFGDQWLAQQSLRTLTNASRRGGLDIKLPL
                     TIYNTSCYRGIPGRYIAAGPLASRWLQQVFATDATLVQSGAVILGEPAAGYVSHEGYA
                     ALARAPYRYQEMLGVIWRENPCRWLKPDESPVLMATLMECDENNQPLAGAYIDRSGLD
                     AETWLTQLFRVVVVPLYHLLCRYGVALIAHGQNITLAMKEGVPQRVLLKDFQGDMRLV
                     KEEFPEMDSLPQEVRDVTSRLSADYLIHDLQTGHFVTVLRFISPLMVRLGVPERRFYQ
                     LLAAVLSDYMKKHPQMSERFALFSLFRPQIIRVVLNPVKLTWPDLDGGSRMLPNYLED
                     LQNPLWLVTQEYES"
     gene            4410..5687
                     /gene="iucD"
                     /locus_tag="NBI74_004044"
     CDS             4410..5687
                     /gene="iucD"
                     /locus_tag="NBI74_004044"
                     /EC_number="1.14.13.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001354579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent L-lysine N(6)-monooxygenase"
                     /protein_id="HCD1934298.1"
                     /translation="MKKSVDFIGVGTGPFNLSIAALSHQIEELDCLFFDEHPHFSWHP
                     GMLVPDCHMQTVFLKDLVSAVAPTNPYSFVNYLVKHKKFYRFLTSRLRTVSREEFSDY
                     LRWAAEDMNNLYFSHTVENIDFDKKRRLFLVQTSQGEYFARNICLGTGKQPYLPPCVK
                     HMTQSCFHASEMNLRRPDLSGKRITVVGGGQSGADLFLNALRGEWGEAAEINWVSRRN
                     NFNALDEAAFADEYFTPEYISGFSGLEEDIRHQLLDEQKMTSDGITADSLLTIYRELY
                     HRFEVLRKPRNIRLLPSRSVTTLESSGPGWKLLMEHHLDQGRESLESDVVIFATGYRS
                     ALPQILPSLMPLITMHDKNTFKVRDDFTLEWSGPKENNIFVVNASMQTHGIAEPQLSL
                     MAWRSARILNRVMGRDLFDLSMPPALIQWRSGT"
     gene            5769..7970
                     /gene="iutA"
                     /locus_tag="NBI74_004045"
     CDS             5769..7970
                     /gene="iutA"
                     /locus_tag="NBI74_004045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004213924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric aerobactin receptor IutA"
                     /protein_id="HCD1934299.1"
                     /translation="MMISKKYTLWALNPLLLTMMAPAVAQQTDDETFVVSANRSNRTV
                     AEMAQTTWVIENAELEQQIQGGKELKDALAQLIPGLDVSSRSRTNYGMNVRGRPLVVL
                     VDGVRLNSSRTDSRQLDSIDPFNIDHIEVISGATSLYGGGSTGGLINIVTKKGQPETM
                     MEFEAGTKSGFSSSKDHDERIAGAVSGGNEHISGRLSVAYQKFGGWFDGNGDATLLDN
                     TQTGLQYSDRLDIMGTGTLNIDESRQLQLITQYYKSQGDDDYGLNLGKGFSAIRGTST
                     PFVSNGLNSDRIPGTERHLISLQYSDSAFLGQELVGQVYYRDESLRFYPFPTVNANKQ
                     VTAFSSSQQDTDQYGMKLTLNSKPMDGWQITWGLDADHERFTSNQMFFDLAQASASGG
                     LNNKKIYTTGRYPSYDITNLAAFLQSDYDINNLFTLNGGVRYQYTENKIDDFIGYAQQ
                     RQIAAGKATSADAIPGGSVDYDNFLFNAGLLMHITERQQAWLNFSQGVELPDPGKYYG
                     RGIYGAAVNGHLPLTKSVNVSDSKLEGVKVDSYELGWRFTGNNLRTQIAAYYSISDKS
                     VVANKDLTISVVDDKRRIYGVEGAVDYLIPDTDWSTGVNFNVLKTESKVNGTWQKYDV
                     KTASPSKATAYIGWAPDPWSLRVQSTTSFDVSDAQGYKVDGYTTVDLLGSYQLPVGTL
                     SFSIENLFDRDYTTVWGQRAPLYYSPGYGPASLYDYKGRGRTFGLNYSVLF"
     gene            complement(8521..8664)
                     /locus_tag="NBI74_004046"
     CDS             complement(8521..8664)
                     /locus_tag="NBI74_004046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001421454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1934300.1"
                     /translation="MEHRSVIHDELAIDHDAILQCAEEVLHRCVIQTVSFVRHILCYS
                     MGL"
BASE COUNT         2043 a         2279 c         2328 g         2061 t
ORIGIN      
        1 gaccctgccc tctgaaaaac cagccacaga tgtggctgcg cagtgcttcc tgaatgcact
       61 gattcgtgaa accacagact ggaaactgac agaatacccg ccagacgaat tgctcatccc
      121 gctggatgag cagaaatcgc tccatttcag agtggcttat ttctccccaa cccagcatca
      181 ccgctttgca tttcctgcac gtctggtcac ggcatcaggc agttatcctg tcgactttac
      241 cactctctcc cggcttatta ttgataagct acgccatcaa ctgtttctgc ccgttcccct
      301 ctgcgaaact ttccaccagc gcgtgctgga aagccatgtc catacgcaac aggcaattga
      361 tgcccgtcat gactgggccg ctctgcgtga aaaagcgttg aattttggcg aggctgagca
      421 ggcgctgctg acaggacacg ctttccaccc tgcgcctaag tctcatgaac cgtttaaccg
      481 gcgggaggct gaacgctacc tgcctgatat ggcaccccac ttcccactgc ggtggttttc
      541 ggtggataaa acgcaaatcg ccggtgaaag tttgcatctc aaccttcaac agcggctgac
      601 gcgatttgcc gcagagaatg cgcctcagtt actcaacgaa ttaagtgaca accaatggct
      661 gttcccgttg cacccgtggc agggggaata tcttttgcag caggggtggt gccaggcact
      721 tgttgctaaa gggctgatta aagacttagg tgaggccggc acgtcgtggc tgccgaccac
      781 ctcttcccgt tccctctact gtgccaccag ccgcgatatg atcaagttct ccctgagcgt
      841 acggctgacc aactccatcc gtactctgtc cgtgaaagaa gtgaagcgtg ggatgcgcct
      901 ggcacgcctg gctcaaaccg acggctggca gatgctacag gtccgcttcc cgactttccg
      961 ggtaatgcag gaggatggct gggccgggct gcgcgatctt aacggcaaca tcatgcagga
     1021 aagtctgttt gccctgcgtg aaaatctgct ggtggatcag ccgaaaagcc agactaacgt
     1081 actggtctcc ctgactcagg ccgcacctga tggcggtgat tcgctgctgg tttcggcggt
     1141 aaaacgcctg agcgatcgcc tcggtatcac tgtgcaacag gccgcccatg catgggtcga
     1201 tgcatactgt cagcaagtgc taaagccgct gtttacggct gaagcggatt acggcctggt
     1261 gctgctggcg catcagcaaa atattcttgt ccagatgctt ggggatctgc cggtcggatt
     1321 tatttaccgt gactgtcagg gcagcgcttt tatgcctcac gcgacagact ggctcgattc
     1381 tattggcgag gcgcaggcgg aaaatatttt cacccatgag cagttgctgc gctatttccc
     1441 ttattacctg ctggttaact ccacttttgc tgtgaccgct gcgctggggg ctgccgggct
     1501 ggacagcgaa tcgaatctga tggctcgtgt acgagcatcg ctggctgaag tgcgtgatca
     1561 ggtgactcat aaaacctgtc tcaactacgt gctggaaagt ccgtactgga acgtaaaagg
     1621 taactttttc tgttatctga acgatcataa cgagaacacc attgttgacc cttcggtgat
     1681 ctatttcgat ttcgctaacc cgctgcaggc tcaggaggtc tgaatgtctg aggcaaacat
     1741 tattcacagc agatatggac tgcgctgtga aaaactcgac aagcccctga atcttggctg
     1801 gggactggac aatagcgcgg tgttgcactg tcccggggag ctgccgacag ggtggctgtg
     1861 cgatgcgctg gatcagatat ttatcgccgc tccacaactg tcagcagtgg ctctcccctg
     1921 ggccgaatgg cgtgaggagc cacaggcgct gacgcttttt gggcaggtaa aaagcgacat
     1981 tatccatcgt actgccttct ggcagttacc gttatggttg agttcaccgg caaaccgggc
     2041 ctccggtgaa atggtttttg atgcagagcg tgagatttat ttcccgcagc gcccaccccg
     2101 tccacagggt gaggtttacc gccgttacga tccacgtatt cgcaggatgc tgagtttccg
     2161 cattgccgat cccgtttctg atgcagaacg tttcacccgc tggatgaatg atccgcgcgt
     2221 tgagtatttc tgggagcaaa gtggctcact ggaggtacag accgcctatc tggagcgcca
     2281 gctcaccggt aaacatgcat tcccgctgat tgggtgcttc gacgatcggc cattcagcta
     2341 tttcgaaatc tactgggcgg cggaagaccg cattggtcgt cactattcgt ggcagccctt
     2401 tgaccgtggc ctgcatctgc tggttggtga acagcaatgg cgcggggccc actatgtgca
     2461 aagctggctg cgcgggctga cacattacct gctgctggat gagccccgta cgcagcgcac
     2521 agtactggag ccacgcaccg ataacctgcg cctgttccgc catcttgagc ctgcgggata
     2581 ccggacaatt aaagagttcg acttcccaca taagcgctcg cgcatggtga tggcggatcg
     2641 tcatcacttc ttcacggagg tcggtctgta atgaatcaca aggactggga ttttgttaac
     2701 cgtcggctgg tggcaaaaat gttgtctgag atggagtatg agcaggtttt ccacgcggaa
     2761 tctcagggcg atgaccacta ctgcattaac ctgccgggag cacaatggcg cttcatcgct
     2821 gaacgtggta tctggggctg gctgtggatt gatgcccaaa cgttgcgctg cacggacgaa
     2881 ccagtactgg cgcagacgct gctgatgcag ctaaagccgg tgctgtcaat gagtgatgcc
     2941 actgttgcag agcatatgca ggatttgtat gccacgctac tgggcgacct gcaactgctg
     3001 aaggcccgtc gcggactgag tgccagtgac ctgattaatc ttgatgccga tcgtctgcaa
     3061 tgcctgctga gcggtcatcc taaattcgtt tttaataaag gtcgccgtgg ctggggcaaa
     3121 gaggcgctgg aacgatatgc gccagagtat accaacacct tcagactgca ctggctggcg
     3181 gtaaaacgtg aacatatgat ctggcgctgc gataacgatc tggatattca gcagttgttg
     3241 actgccgcaa tggatccgca ggagtttaca cgcttcagtc aggtctggca ggaaaacgga
     3301 ctggatcaca actggctgcc gctgccggta catccgtggc agtggcagca aaaaatcgct
     3361 accgacttca tcgctgattt tgccgaaggc aggatggtgt ctctcggcga gtttggcgac
     3421 cagtggctcg cccagcagtc gctgcgtacc ctgaccaacg ccagtcgccg gggcgggctg
     3481 gatatcaagc tgccgctgac catctacaac acctcatgct accgggggat ccctggcaga
     3541 tacatcgctg ccggaccact ggcttcacgc tggctacaac aggtttttgc gaccgacgcc
     3601 accctagtgc aaagcggcgc agtgatactt ggtgaaccgg ctgcaggcta tgtctcccat
     3661 gaaggctatg ccgcgcttgc ccgggctccc tatcgctacc aggaaatgct tggtgttatc
     3721 tggcgggaga atccgtgccg ctggctgaaa ccggatgaaa gtccggttct gatggcaaca
     3781 ctgatggagt gcgacgaaaa caatcagccg ctggcaggcg catatataga ccgctccggg
     3841 ctggacgctg aaacctggct tacgcaactg ttccgggtgg tcgtggttcc tctgtatcac
     3901 ctgctttgcc gctacggtgt cgcgcttatt gcacatggac aaaatataac tctcgccatg
     3961 aaagaggggg ttccacagcg tgttctgctg aaagacttcc agggcgatat gcgactggtg
     4021 aaagaagagt tccccgaaat ggactctttg cctcaggagg ttcgtgatgt tacatcccgc
     4081 ctgagtgcgg actacttaat ccatgatttg cagacgggtc acttcgtgac agtactgcgt
     4141 tttatttcgc cactgatggt tcgtcttggc gtacctgaaa ggcgatttta tcaactgctg
     4201 gcagcagtgt tgagtgatta catgaaaaaa catccacaaa tgtcagagcg ttttgcgctt
     4261 ttctcacttt tcaggccaca aatcattcgc gtggtgctga acccggtaaa actgacctgg
     4321 cctgatctgg atggcggcag ccgcatgctg ccgaattacc ttgaggatct gcaaaatccg
     4381 ctgtggctgg taactcagga atatgaatca tgaaaaaaag tgtcgatttt attggtgtag
     4441 ggacagggcc atttaatctc agcattgctg cgttgtcaca tcagatcgaa gaactggact
     4501 gtctcttctt tgatgaacat cctcattttt cctggcatcc gggtatgctg gtaccggatt
     4561 gtcatatgca gaccgtcttt ctgaaagatc tggtcagtgc tgttgcacct acaaatccct
     4621 acagttttgt taactatctg gtgaagcaca aaaagttcta tcgcttcctt acaagcagac
     4681 tacgtacagt atcccgtgaa gagttttctg actacctccg ctgggctgct gaagatatga
     4741 ataacctgta tttcagtcat accgttgaaa acattgattt cgataaaaaa cgtcgattgt
     4801 ttctggtgca aaccagccag ggagaatatt ttgcccgcaa tatctgcctt ggtacaggaa
     4861 aacaacctta tttaccaccc tgtgtgaagc atatgacaca atcctgtttc catgccagtg
     4921 aaatgaatct tcgtcggccg gaccttagtg gaaaacggat aaccgtggtt ggtggaggac
     4981 agagtggtgc agacctgttc cttaatgcat tacgcgggga atggggagaa gcggcggaaa
     5041 taaactgggt ctcccggcgt aataatttta acgcactgga tgaggctgct tttgctgatg
     5101 agtattttac acctgaatat atttcaggct tctccggact ggaggaagat attcgccatc
     5161 agttactgga tgagcagaaa atgacatcgg atggcatcac tgccgattct ttactgacca
     5221 tttatcgtga gttgtaccac cgttttgaag ttctgagaaa accaagaaat atccgtctgc
     5281 tacccagccg ctcggtaaca actctggaaa gtagtggtcc tggctggaag ttgctgatgg
     5341 agcatcatct ggatcagggc agggagagcc tggaaagtga tgtggtgatt ttcgccacag
     5401 gttaccgttc tgcgttgcca caaatacttc cctcactgat gcccctgatc accatgcacg
     5461 ataagaacac ctttaaagtg cgtgatgact tcactctgga atggagtggc ccgaaagaga
     5521 acaacatctt cgtggtcaac gccagtatgc aaacccatgg catcgccgaa ccccagctca
     5581 gcctgatggc atggagatct gcacgtattc ttaatcgcgt aatgggacgt gatttattcg
     5641 atctcagtat gccgcccgcc ctgattcagt ggcgcagcgg cacctaggga aaacgcagcc
     5701 ggaggctgct tctttaactc gctacacagc atctttgggc tgattttttc cgcccgtatg
     5761 gaggaataat gatgataagc aaaaagtata cgctttgggc tctcaaccca ctgcttctta
     5821 ccatgatggc gccagcagtc gctcaacaaa ccgatgatga aacgttcgtg gtgtctgcca
     5881 accgcagcaa tcgcaccgta gcggagatgg cgcaaaccac ctgggttatc gaaaacgccg
     5941 aactggaaca gcagattcag ggcggcaaag agcttaaaga cgcactggct cagctgatcc
     6001 ctggccttga cgtcagcagc cggagccgca ccaactacgg tatgaatgtg cgtggccgcc
     6061 cgctggtcgt gctggttgac ggcgtgcgtc tcaactcttc acgtaccgac agccgacaac
     6121 tggactctat agatcctttt aatatcgacc atattgaagt gatctccggt gcgacgtccc
     6181 tgtacggcgg cggcagtacc ggtggcctga tcaacatcgt gaccaaaaaa ggccagccgg
     6241 aaaccatgat ggagtttgag gctggcacca aaagtggctt tagcagcagt aaagatcacg
     6301 atgaacgcat tgccggagct gtctccggcg gaaatgagca tatctccgga cgtctttccg
     6361 tggcatatca gaaatttggc ggctggtttg acggtaacgg cgatgccacc ttgcttgata
     6421 acacccagac cggcctgcag tactccgatc ggctggacat catgggaact ggtacgctga
     6481 acatcgatga atcccggcag cttcagttga tcacacagta ctataaaagc cagggcgacg
     6541 acgattacgg gcttaatctc gggaaaggct tctctgccat cagagggacc agcacgccat
     6601 tcgtcagtaa cgggctgaat tccgaccgta ttcccggcac tgagcggcat ttgatcagcc
     6661 tgcagtactc tgacagcgct tttctgggac aggagctggt cggtcaggtt tactaccgcg
     6721 atgagtcgtt gcgattctac ccgttcccga cggtaaatgc gaacaaacag gtgacggctt
     6781 tctcttcgtc acagcaggac accgaccagt acggcatgaa actgactctg aacagcaaac
     6841 cgatggacgg ctggcaaatc acctgggggc tggatgctga tcatgagcgc tttacctcca
     6901 accagatgtt cttcgacctg gctcaggcaa gcgcttccgg agggctgaac aacaagaaga
     6961 tttacaccac cgggcgctat ccgtcgtatg acatcaccaa cctggcggcc ttcctgcaat
     7021 cagactatga catcaataat ctctttaccc tcaacggtgg cgtacgctat cagtacactg
     7081 aaaacaagat tgatgatttc atcggctacg cgcagcaacg gcagattgcc gccgggaagg
     7141 ctacatccgc cgacgccatt cctggcggct cagtcgatta cgacaacttc ctgttcaacg
     7201 ccggtctgct gatgcacatc accgaacgcc agcaggcatg gctcaacttc tcccagggcg
     7261 tggagctgcc ggacccgggt aaatactatg gtcgcggcat ctatggtgct gcagtgaacg
     7321 gccatcttcc tctaacaaag agtgtgaacg tcagcgacag caagctggaa ggcgtgaaag
     7381 tcgattctta tgagctgggc tggcgcttta ctggcaataa tctgcgcacc caaatcgcgg
     7441 cctactattc gatttctgat aagagcgtgg tggcgaataa agatctgacc atcagcgtgg
     7501 tggacgacaa acgccgtatt tacggcgtgg aaggtgcggt ggactacctg attcctgata
     7561 ctgactggag taccggagtg aacttcaacg tgctgaaaac tgagtcgaaa gtgaacggta
     7621 cctggcagaa atacgatgtg aagacagcaa gcccatcaaa agcgacagcc tacattggct
     7681 gggcaccgga cccgtggagt ctgcgcgtgc agagcaccac ctcctttgac gtgagcgacg
     7741 cgcagggcta caaggtcgat ggctatacca ccgtggatct gctcggcagt tatcagcttc
     7801 cggtgggtac actcagcttc agcattgaaa acctcttcga ccgtgactac accactgtct
     7861 gggggcagcg tgcaccactg tactacagcc cgggttacgg cccagcgtca ctgtacgact
     7921 acaaaggcag gggccgaacc tttggtctga actactctgt gctgttctga cggggatgct
     7981 tttgcaacaa gggtatgctg taaacaacat ggtccttggt ggccatgttg tttacagcca
     8041 gtataatata cagccttaac aagatattcc gtctccggaa tggactgttc accactgaat
     8101 aacattatgt ctgttttatg gatctttgac tgaaatttat cgcatatcct catttcattg
     8161 tccgtctgaa aactaacctt acaagtaagc gtcgtctcag caccgtctgg cagatcctga
     8221 aattcctgag aggatgatct gcgcccaata tcctgagccg gaactttgct gcaatgaagg
     8281 ccaacgaaaa atgggggagt atgttataga gtacttgcta cagggtaaaa agaggtacct
     8341 gtcgctggtt ctccgttcag tttaaccggg agattttctc ctgcgcctgt cataaagtcc
     8401 ggtaatggag attgttaata cgatggtgca cgatgcattg tcagtgcccg gcccggagga
     8461 tgccccgtag ctacatacgg atcagggttg gcaatatcga atggcaggct ataggcaaag
     8521 ttaaagcccc atggagtagc acaaaatatg ccgcacaaag gaaacggtct gaataacgca
     8581 gcgatggaga acttcttcgg cacactgcaa aatcgcatcg tggtcgatcg ctaattcgtc
     8641 gtgaatcact gaacgatgtt ccatgcgaac agatattcga tcatatcgaa atattaaaca
     8701 ttacccctcc c
//
LOCUS       DAHHTG010000165         8620 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.168, whole genome shotgun sequence.
ACCESSION   DAHHTG010000165 DAHHTG010000000
VERSION     DAHHTG010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8620)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8620)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8620
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.168"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            218..1339
                     /gene="ompC"
                     /locus_tag="NBI74_004047"
     CDS             218..1339
                     /gene="ompC"
                     /locus_tag="NBI74_004047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HCD1934301.1"
                     /translation="MKVKVLSLLVPALLVAGAANAAEVYNKDGNKLDLYGKVDGLHYF
                     SDNKSEDGDQTYMRLGFKGETQVTDQLTGYGQWEYQIQGNSAENENNSWTRVAFAGLK
                     FQDVGSFDYGRNYGVVYDVTSWTDVLPEFGGDTYGSDNFMQQRGNGFATYRNTDFFGL
                     VDGLNFAVQYQGKNGSVDGEGMTNNGRGALRQNGDGVGGSITYDYEGFGIGAAVSSSK
                     RTDDQNFGLNGYGERYLGNGDRAETYTGGLKYDANNIYLAAQYTQTYNATRVGSLGWA
                     NKAQNFEAVAQYQFDFGLRPSVAYLQSKGKNLGVINGRNYDDEDILKYVDVGATYYFN
                     KNMSTYVDYKINLLDDNRFTRDAGINTDDIVALGLVYQF"
     gene            1451..2506
                     /gene="apbE"
                     /locus_tag="NBI74_004048"
     CDS             1451..2506
                     /gene="apbE"
                     /locus_tag="NBI74_004048"
                     /EC_number="2.7.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase ApbE"
                     /protein_id="HCD1934302.1"
                     /translation="MEISFTRVALLAAALFFVGCDQKPQPAKTHATEVTVLEGKTMGT
                     FWRASIPGIDAKRSAELKEKIQTQLDADDQLLSTYKKDSALMRFNDSQSLSPWPVSEA
                     MADIVTTSLRIGARTDGAMDITIGPLVNLWGFGPEQQPVQIPSQEQIDAMKAKTGLQH
                     LTVINQSHQQYLQKDLPDLYVDLSTVGEGYAADHLARLMEQEGISRYLVSVGGALNSR
                     GMNGAGQPWRVAIQKPTDKENAVQAVVDINGHGISTSGSYRNYYELDGKRLSHVIDPQ
                     TGRPIEHNLVSVTVIAPTALEADAWDTGLMVLGPEKAKEVVRREGLAVYMITKEGDNF
                     KAWMSPQFKSFLISEKN"
     gene            2580..3644
                     /gene="ada"
                     /locus_tag="NBI74_004049"
     CDS             2580..3644
                     /gene="ada"
                     /locus_tag="NBI74_004049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-binding transcriptional
                     regulator/O6-methylguanine-DNA methyltransferase Ada"
                     /protein_id="HCD1934303.1"
                     /translation="MKYAIRLTDDQRWQSVLARDSNADGEFVFAVRTTGIFCRPSCRA
                     RHALRENVSFYANASEALAAGFRPCKRCQPDKANPLQHRLDKITHACRLLEQETPVTL
                     EALADQVAMSPFHLHRLFKATTGMTPKAWQQAWRARRLRELLAKGESVTTSILNAGFP
                     DSSSYYRKADETLGMTAKQFRHGGENLAVRYALADCELGRCLVAESERGICAILLGDD
                     DATLISELQQMFPAADSAPADLTFQQHVREVIASLNQRDTPLTLPLDIRGTAFQQQVW
                     QALRTIPCGETVSYQQLANAIGKPKAVRAVASACAANKLAIVIPCHRVVRGDGSLSGY
                     RWGVSRKAQLLRREAENEER"
     gene            3644..4294
                     /gene="alkB"
                     /locus_tag="NBI74_004050"
     CDS             3644..4294
                     /gene="alkB"
                     /locus_tag="NBI74_004050"
                     /EC_number="1.14.11.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA oxidative demethylase AlkB"
                     /protein_id="HCD1934304.1"
                     /translation="MLDLFADAEPWQEPLAAGAVILHRFAFNAAEQLIRDINDVASQS
                     PFRQMVTPGGYTMSVAMTNCGHLGWTTHRQGYLYSPIDPQTNKPWPAMPQSFHHLCQR
                     AATAAGYPDFQPDACLINRYVPGAKLSLHQDKDEPDLRAPIVSVSLGLPAIFQFGGLK
                     RNDPLKRLLLEHGDVVVWGGESRLFYHGIQPLKVGGHPLTTDCRYNLTFRQAGKKE"
     gene            4370..6013
                     /gene="yojI"
                     /locus_tag="NBI74_004051"
     CDS             4370..6013
                     /gene="yojI"
                     /locus_tag="NBI74_004051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin J25 efflux ABC transporter YojI"
                     /protein_id="HCD1934305.1"
                     /translation="MELLVLVWRQYRWPFISVMALSLASAALGIGLIAFINQRLIETA
                     DTSLLVLPEFLGLLLLLMAVTLGSQLALTTLGHHFVYRLRSEFIKRILDTHVERIEQL
                     GSASLLAGLTSDVRNITIAFVRLPELVQGIILTIGSAAYLWMLSGKMLLVTAIWMAVT
                     IWGGFILVARVYKHMATLRETEDKLYTDFQTVLEGRKELTLNRERAEYVFNNLYIPDA
                     QEYRHHIIRADTFHLSAVNWSNIMMLGAIGLVFWMANSLGWADTNVAATYSLTLLFLR
                     TPLLSAVGALPTLLTAQVAFNKLNKFALAPFKAEFPRPQAFPNWQTLELRNVTFSYQD
                     NAFSVGPINLTIKRGELLFLIGGNGSGKSTLAMLLTGLYQPQNGEILLDGKPVSGEQP
                     EDYRKLFSAVFTDVWLFDQLLGPEGQPANPQLVEKWLAQLKMAHKLELSNGRIVNLKL
                     SKGQKKRVALLLALAEERDIILLDEWAADQDPHFRREFYQVLLPLMQEMGKTIFAISH
                     DDHYFIHADRLLEMRNGQLSELTGEERDAASRDAVARTA"
     gene            6231..7877
                     /gene="mqo"
                     /locus_tag="NBI74_004052"
     CDS             6231..7877
                     /gene="mqo"
                     /locus_tag="NBI74_004052"
                     /EC_number="1.1.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase (quinone)"
                     /protein_id="HCD1934306.1"
                     /translation="MKKVTAMLFSMAVGLNAVSMAAKAKASEEQETDVLLIGGGIMSA
                     TLGTYLRELEPEWSMTMVERLEGVAQESSNGWNNAGTGHSALMELNYTPQNADGSISI
                     EKAVAINEAFQISRQFWAHQVERGVLRTPRSFINTVPHMSFVWGEDNVNFLRARYAAL
                     QQSSLFRGMRYSEDHAQIKEWAPLVMEGRDPQQKVAATRTEIGTDVNYGEITRQLIAS
                     LQKKSNFSLQLSSEVRALKRNDDNSWTVTVADLKNGTAQNIRAKFVFIGAGGAALKLL
                     QESGIPEAKDYAGFPVGGQFLVSENPDVVNHHLAKVYGKASVGAPPMSVPHIDTRVLD
                     GKRVVLFGPFATFSTKFLKNGSLWDLMSSTTTSNVMPMMHVGLDNFDLVKYLVSQVML
                     SEEDRFEVLKEYYPQAKKEDWRLWQAGQRVQIIKRDADKGGVLRLGTEVVSDQQGTIA
                     ALLGASPGASTAAPIMLNLLEKVFGDRVSSPQWQATLKAIVPSYGRKLNGDVAATERE
                     LQYTSEVLGLKYDKPQAADSTPKPQLKPQPVQKEVADIAL"
     gene            complement(8027..8515)
                     /gene="eco"
                     /locus_tag="NBI74_004053"
     CDS             complement(8027..8515)
                     /gene="eco"
                     /locus_tag="NBI74_004053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease inhibitor ecotin"
                     /protein_id="HCD1934307.1"
                     /translation="MKTILPAVLFAAFATTSAWAAESVQPLEKIAPYPQAEKGMKRQV
                     IQLTPQEDESTLKVELLIGQTLEVDCNLHRLGGKLESKTLEGWGYDYYVFDKVSSPVS
                     TMMACPDGKKEKKFVTAYLGDAGMLRYNSKLPIVVYTPDNVDVKYRVWKAEEKIDNAV
                     VR"
BASE COUNT         2083 a         2138 c         2309 g         2090 t
ORIGIN      
        1 tttgccattc cgcaataatc ttaaaaagtt cccttgtatt tacattttga aacatctata
       61 gcgataaatg aaacatctta aaagttttag tatcatattc gtgttggatt attctgcatt
      121 tttggggaga atggacttgc cgactgatta atgagggtta atcagtatgc agtggcataa
      181 aaaagcaaat aaaggcatat aacagagggt taataacatg aaagttaaag tactgtccct
      241 cctggtccca gctctgctgg tagcaggcgc agcaaacgct gctgaagttt acaacaaaga
      301 cggcaacaaa ttagatctgt acggtaaagt agacggcctg cactatttct ctgacaacaa
      361 gtcagaagac ggcgaccaga cctacatgcg tcttggcttc aaaggtgaaa ctcaggttac
      421 tgaccagctg accggttacg gccagtggga atatcagatc cagggcaaca gcgctgaaaa
      481 cgaaaacaac tcctggaccc gtgtggcatt cgcaggtctg aaattccagg atgtgggttc
      541 tttcgactac ggtcgtaact acggcgttgt ttatgacgta acttcctgga ccgacgtact
      601 gccagaattc ggtggcgaca cttacggttc tgacaacttc atgcagcagc gtggtaacgg
      661 cttcgcgacc tatcgtaaca ccgacttctt cggtctggtt gacggcctga actttgctgt
      721 tcagtaccag ggtaaaaacg gtagcgtaga tggcgaaggc atgaccaaca acggtcgtgg
      781 tgccctgcgt cagaacggcg acggcgttgg cggatctatc acttatgatt acgaaggctt
      841 cggtatcggt gctgctgttt ctagctccaa acgtactgat gatcagaact tcggcctgaa
      901 cggttatggt gagcgttatt tgggtaacgg cgaccgtgct gaaacctaca ctggtggtct
      961 gaaatacgac gctaacaaca tctacctggc tgctcagtat acccagacct acaacgcaac
     1021 tcgcgtaggt tccttgggtt gggcaaacaa agcacagaac ttcgaagctg ttgctcagta
     1081 ccagttcgac ttcggtctgc gtccgtctgt agcctacctg cagtctaaag gtaaaaacct
     1141 gggtgtcatc aatggtcgta actacgacga cgaagatatc ctgaaatatg ttgatgttgg
     1201 tgcgacctac tacttcaaca aaaacatgtc cacctacgtt gactacaaaa tcaacctgct
     1261 ggacgacaac cgcttcaccc gtgacgctgg catcaacact gatgacatcg tagctctggg
     1321 tctggtttac cagttctaat ctcgattgat atcgaacaaa gggcctgcgg gccctttttt
     1381 cattgttttc agcgtacaaa cacagttttt tggtgtactc ttgcgaccgt tcgcatgagg
     1441 ataatcacgt atggaaataa gctttacccg cgtggcactg ctggctgccg cgctcttctt
     1501 tgttggttgc gatcaaaaac cacaacccgc caaaacccac gctactgaag ttaccgttct
     1561 tgaaggcaaa accatgggta ccttctggcg tgccagcatc ccgggcattg acgccaaacg
     1621 cagtgccgaa cttaaagaaa agattcagac ccagctggac gctgacgatc agctgctttc
     1681 gacctataaa aaagattccg cgctgatgcg ctttaacgac tcgcaaagtt tgtcgccgtg
     1741 gccggtaagt gaagcgatgg ccgatatcgt caccacctcg ctgcgcattg gcgcaaggac
     1801 cgatggtgcg atggatataa ccatcgggcc gctggtgaat ctgtggggct ttggcccgga
     1861 acaacaaccg gttcaaattc cgagccagga acagatcgat gcgatgaaag ccaaaaccgg
     1921 cttacagcac ctgacggtca ttaatcagtc gcatcagcaa tatctgcaaa aagacctgcc
     1981 ggatttatat gtcgatctct ccaccgtcgg cgaaggttat gcggcggatc acctggcacg
     2041 cttgatggag caggaaggga tttcccgcta tctggtgtcg gtgggcggcg cgctgaacag
     2101 ccgtggtatg aacggtgcag gccaaccgtg gcgggtagcg attcaaaaac caaccgataa
     2161 agaaaacgcg gttcaggccg tggtggatat caacggtcat ggtattagca cctctggcag
     2221 ctaccgtaac tattacgaac tggacggcaa acgtctttct catgttatcg atccgcaaac
     2281 cgggcgtcct atcgaacaca atctggtatc cgtgacggtg attgccccga cggcgctgga
     2341 ggccgatgcc tgggatactg gcttgatggt actcggaccg gagaaagcca aagaggttgt
     2401 tcgccgggaa ggactggcgg tctacatgat caccaaagag ggcgataatt ttaaagcctg
     2461 gatgtcgccg cagtttaaaa gcttccttat cagcgaaaaa aattaaagcg caagattgtt
     2521 ggtttttgtg cgatggtgac cgggcagact aaaggctatc cttaaccaga gagctgatta
     2581 tgaaatacgc catacgctta actgacgatc aacgctggca atctgtctta gcccgcgact
     2641 cgaatgccga cggcgaattc gttttcgccg tgcgcaccac gggtatcttt tgccgtccgt
     2701 cttgccgagc cagacatgcc ttgcgggaaa atgtctcctt ctacgcaaat gccagcgagg
     2761 cgcttgccgc tggctttcgt ccctgcaaac gttgtcagcc agacaaagcc aatcccctgc
     2821 aacatcgcct tgataaaatt acccacgcct gccgacttct ggaacaggaa acgcctgtaa
     2881 cgctggaagc cttagccgac caggtggcga tgagtccatt tcatttgcat cggttgttta
     2941 aagcgactac cggaatgacg cctaaagcct ggcaacaggc ctggcgcgct cgccgtttgc
     3001 gcgaattgct ggcgaaaggg gagagcgtga ctacatctat tcttaacgcc ggattccccg
     3061 acagcagcag ttactaccgc aaagccgacg aaacgctggg catgacggct aaacaattcc
     3121 gtcacggtgg cgaaaatctg gcggtgcgtt acgcgctggc tgattgtgag ctgggccgtt
     3181 gcctggtggc agaaagtgag cgggggattt gcgcgatatt actgggcgat gatgacgcga
     3241 cactaatcag cgagttacag cagatgtttc ccgctgccga cagcgcgcct gctgatctga
     3301 cgtttcagca acatgtgcgt gaagtgatcg ccagcctcaa tcaacgcgat acgccgctga
     3361 cgttaccgct ggacattcgc ggcactgctt ttcagcaaca agtctggcag gcactgcgca
     3421 cgataccttg cggtgaaacc gtcagttatc agcaactggc taacgccatc ggcaaaccga
     3481 aagcggtacg ggctgttgcc agcgcctgtg ccgccaacaa gctagctatc gttataccct
     3541 gtcatcgggt agtacgcggt gatggatcgc tttcaggtta ccgctggggc gtgtcgcgta
     3601 aagcgcaact gctacgccgc gaagctgaaa atgaggagag gtaatgttgg atctgtttgc
     3661 cgatgctgaa ccgtggcaag aaccactggc ggctggagcg gtgattttgc accgttttgc
     3721 ttttaacgct gcggagcaac tgatccgcga tattaatgac gttgccagcc agtcgccgtt
     3781 tcgccagatg gtcactccag gcggatatac catgtcggtg gcgatgacca actgtgggca
     3841 tctgggctgg acgacccatc ggcaaggtta tctctattcg cccattgatc cgcaaacaaa
     3901 taaaccgtgg cccgccatgc cgcagagttt tcatcattta tgtcaacgtg cggctacggc
     3961 agcgggctat ccagatttcc agccagatgc ctgtttgatc aaccgctacg ttcctggcgc
     4021 gaaactgtcg ctgcatcagg ataaagacga accggatctg cgcgcgccaa ttgtttctgt
     4081 ttctctgggc ttacccgcga tttttcaatt tggcggcctg aaacgaaatg atccgctcaa
     4141 acgtttgttg ttggaacatg gcgatgtggt ggtatggggt ggtgagtcgc ggctgtttta
     4201 tcatggcatt caaccgttga aagtgggggg gcatccactc accaccgact gccgctacaa
     4261 cctgacattc cgtcaggcag gtaaaaaaga ataaaaataa gaattattat tgctgtgcgc
     4321 acgaagatgt ttaaactgcg ggctgtaatt cattgtccgg gttttctgca tggaacttct
     4381 tgtacttgtc tggcggcagt atcgctggcc atttatcagt gtgatggcgc taagcctcgc
     4441 cagtgcggca ttaggcattg gcttaattgc ttttatcaat cagcgcctta tcgaaacggc
     4501 ggataccagt ctgctggtat tgccggagtt tctggggtta ttgctgctgt tgatggcagt
     4561 cactctcgga tcgcaactgg cgctcaccac tttggggcat cacttcgttt accgactgcg
     4621 tagtgaattt atcaagcgga ttctggatac ccatgtcgag cgcattgaac aacttggtag
     4681 cgcctcgttg ctggcggggt taaccagcga tgtgcgcaat atcaccattg cttttgtgcg
     4741 tctgccggaa ctggtgcagg ggatcattct cactatcggt tcagcggcgt atttgtggat
     4801 gctgtcgggc aaaatgttgc tggtaacggc tatctggatg gcagtcacca tctggggtgg
     4861 ttttatactg gtggcgcggg tgtacaaaca tatggcgacc ctgcgtgaaa ccgaagataa
     4921 gctgtacacg gattttcaaa ccgtactcga agggcgcaaa gagctgactc tgaaccgtga
     4981 acgcgctgag tatgtgttta acaacctcta cattcctgat gcgcaagagt atcgccatca
     5041 tattatccgc gcagacacct tccatcttag tgccgtgaac tggtcaaaca tcatgatgct
     5101 gggcgcaatc ggcctggtgt tctggatggc gaacagtctc ggttgggctg ataccaacgt
     5161 tgccgcgacc tattcgttga cgttgttatt cctgcgtacg ccgctgcttt cggcggttgg
     5221 cgcgttgccg acgctactga cggcgcaggt ggcgtttaat aagctgaaca aattcgcgct
     5281 cgcgcctttc aaagcagagt ttccgcgccc gcaggcgttt cccaactggc aaacgctgga
     5341 gctgcgtaac gtgacgttta gctatcagga taacgcgttt tccgttggtc caattaatct
     5401 caccattaaa cgtggcgagc tgctgtttct gattggtggc aacggtagtg gaaaatcgac
     5461 gctggcgatg ttgctgacgg gcttgtacca gccacaaaat ggcgaaatct tgctggatgg
     5521 caaacctgtc agcggcgaac aaccggaaga ttatcgaaaa ctgttttcgg cagtgtttac
     5581 cgatgtctgg ctgtttgatc aactgctggg gccggagggg caacccgcta acccgcaact
     5641 ggttgagaag tggctggcgc agctgaaaat ggctcataag cttgagttaa gcaacgggcg
     5701 tattgttaac ctgaagttat caaaagggca gaaaaaacgc gtggcgctgt tgctggcgct
     5761 ggcagaagaa cgcgatatta tcctgctgga tgaatgggcg gcggatcagg atccacactt
     5821 ccgtcgtgag ttttatcagg tgctgctgcc gctgatgcag gagatgggta aaactatttt
     5881 cgccatcagt catgatgatc attactttat ccacgccgac cgcctgctgg aaatgcgcaa
     5941 cgggcaactt agcgagctga caggcgaaga gcgcgatgct gcttcgcgtg atgccgttgc
     6001 ccggacagca taatattacg acgctgacct ggcggactgc tgccgccagg tcaatattct
     6061 aaaaatacct tcgtttctaa taaccctata attaatcaac gaaattataa tgtttctaaa
     6121 attagaatat aatttataaa cattatttaa atgttgttac ttaagtgtta ccgttgatgc
     6181 cgcgcaaatc tcacctgcaa taaagcgact aaaagtaagg cattaacaag atgaaaaaag
     6241 tgactgccat gctcttctcg atggccgtgg ggcttaatgc cgtttcgatg gcggcaaaag
     6301 cgaaagcgtc cgaggagcag gaaactgatg tactgttgat tggcggcggc attatgagcg
     6361 ccacgttggg gacctattta cgcgagctgg agcctgaatg gtcgatgacc atggtggagc
     6421 gcctggaagg tgtcgcgcag gagagttcga acggctggaa caacgccgga accgggcatt
     6481 ctgcactgat ggaactcaat tacaccccgc aaaatgccga cggcagtatc agtattgaaa
     6541 aagcagtcgc cattaacgaa gcatttcaga tttcccgcca gttctgggcg caccaggtcg
     6601 agcgtggtgt gctgcgtact ccgcgttcat ttatcaatac cgttccgcat atgagctttg
     6661 tctggggcga ggataacgtt aatttcctgc gcgcccgtta cgccgcgttg caacaaagct
     6721 cgctgtttcg cggtatgcgt tactctgaag atcacgcgca gatcaaagag tgggcaccgt
     6781 tagtgatgga agggcgcgat ccgcaacaga aagtagcggc aacacgcaca gaaattggca
     6841 ccgatgtgaa ctatggcgag atcacccgcc agttgatcgc ctccttacag aagaaatcta
     6901 acttctcgct gcaactcagc agcgaagtcc gcgccctaaa gcgtaatgac gataacagct
     6961 ggaccgtcac cgttgccgat ctgaaaaacg gcactgcaca gaacatccgc gccaaatttg
     7021 tctttatcgg cgcgggcggt gcggcgctga aactgttaca ggaatcgggg attccggagg
     7081 cgaaagacta cgctggtttc ccggtgggcg gacagttcct tgtttcggaa aacccggacg
     7141 tggttaatca ccatctggcg aaggtttacg gtaaagcatc cgttggcgca ccaccgatgt
     7201 cggttccgca tatcgatacc cgtgttcttg acggtaaacg cgtagtgctg tttgggccat
     7261 ttgccacctt ctcaaccaaa ttcctcaaaa acggttcatt atgggatctg atgagttcca
     7321 ccaccacctc taacgtgatg ccgatgatgc acgttgggct ggataatttc gatctggtga
     7381 aatatctggt gagtcaggtg atgttgagtg aagaggatcg ttttgaagtg ttgaaagagt
     7441 actatccgca ggcgaaaaaa gaggactggc gtttgtggca agcggggcag cgcgtgcaga
     7501 ttatcaagcg tgatgccgat aaaggtggcg tactgcgtct cggtactgaa gtcgtcagtg
     7561 accagcaagg gaccattgcc gcgctcctgg gggcatcgcc aggggcgtca accgccgcgc
     7621 cgattatgtt gaatctgctg gaaaaagtat ttggcgatcg cgtttccagc ccgcaatggc
     7681 aggctacgtt gaaagcgatc gttccgtctt atggacgcaa gctgaacggt gatgtagcgg
     7741 caacagaacg cgagttgcag tacaccagcg aagtgctggg tctgaaatac gacaagccgc
     7801 aggcagcaga tagtacgccg aaaccgcagt tgaaaccgca acccgttcaa aaagaagtgg
     7861 cggatattgc gttgtaatga tgtgccacat ccggcatggt ttgccggatg tggcgtatgc
     7921 tgataagacg cttcagcgtc gcatcaggca accggctcgg gcgttagatg tcggatgcgg
     7981 cgtaaccgcc ttattcggcc tacaggttgc agcacttcaa agcagtttag cgaacaaccg
     8041 cgttgtcaat tttctcttcc gccttccaga cacggtactt cacatccaca ttgtctggcg
     8101 tatacaccac gatcggcagc ttgctgttgt aacgcagcat tccagcatcg cccagatacg
     8161 cggtgacaaa tttcttctct ttcttgccat ccgggcaggc catcatcgtg gaaaccggcg
     8221 agctaacttt atcaaagaca taataatcat agccccagcc ttccagcgtt ttgctttcca
     8281 gcttcccgcc gagacgatgc aaattgcagt cgacttccag cgtctgaccg attaacagtt
     8341 ctactttcag ggtagattca tcttcttgcg gagttaactg aatcacctga cgcttcatgc
     8401 ctttttcagc ttgtggataa ggcgcgattt tttccagtgg ctggacgctc tctgccgccc
     8461 aggcggaagt ggtggcgaaa gcggcaaaca atactgcagg tagaatggtc ttcatttttt
     8521 tgctttcctt cttcatttat aggttttcac cgatgatgtc gctgaaggtt aacatattca
     8581 tcagatttat taatttgctt tatgaataac ttaaagaaca
//
LOCUS       DAHHTG010000166         8434 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.169, whole genome shotgun sequence.
ACCESSION   DAHHTG010000166 DAHHTG010000000
VERSION     DAHHTG010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8434)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8434)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8434
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.169"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            42..1256
                     /locus_tag="NBI74_004054"
     CDS             42..1256
                     /locus_tag="NBI74_004054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001145249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1934308.1"
                     /translation="MPVRLNTIPDAEPYPVPPARIKWLTALLLMLSVGVALTSLFASD
                     ELAKNGPYFWGLACGVPAFIWSLVASVRWLFFITQYIRTDAWNKRREEVILQETRRGR
                     RALQILSFSVQTALNGDSVAETITAFLARQQVLNTYADLRGEDTVRRSVIPVLTNKPV
                     TGRLSEVFSRLFSDIRLQLTRLPDDFHINVLLEIDAPLSGASVRTIWQDEWQKAGLPE
                     ARLFSAPEPGLAAVDDWLDNHIQEKAVLLVISVRLEPKNPERTAESATALLLANRLTQ
                     TALTPLALLHRPECITDTEMMASGIAQALDWMPVQPDAISGMWTAELDREQRAALLSL
                     NQPFAQEALMYELDAFLGRSGPAAPWLSVAVATLAAIQSQHPQLTLSGVQGGHYSWAT
                     VVSPFVSPQEAS"
     gene            1313..4645
                     /locus_tag="NBI74_004055"
     CDS             1313..4645
                     /locus_tag="NBI74_004055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001335663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion protein VasK"
                     /protein_id="HCD1934309.1"
                     /translation="MTVAVVVALCTFIHRKPALLGVEPGSSQQMYWMAGVIAAGVVFL
                     IIMGVYHLLNRHAAVQNFELRRQLAAGDDYRAVTTKKADSRHPAMVELAAFLRDRYGP
                     FWRRKIRLLLVTGEPEQAEAIAPGLTGQHWLEGDHTVLIYGGRPSAEPDVALLTALKK
                     LRRSRPLDGIIWPLTEEQSRQTAQLDKGWRELINGGKRLGFQAPFYLWQVCDNGDYQS
                     GRVLQSVGCLLPEGCTPEQLAAMLEAQTLPLTEQGMSQLLTDNRHDFLLRLAHTLAER
                     GITHWQTVLKPLLAGGAFSSLRLRGLMFSPPLAAVPEAAPHAWLPSPVWAGITGDNAR
                     GRAVGFPWLRTALMSAVCVLAIGGAGMTTSFFANRALVQETSIQTARALDTRLPLAEQ
                     LVALHTLQGELERLQYRIREGAPWYQRFGPERNQQLLAAAFPGYAQAANRLVRDVAVD
                     HLQQQLNAFVALPPNSPQRTATGEQRYKQLKALLMTSRPEKADAAFFSTTLMADGLRY
                     ENIPEGVRQSVLPSLLTFWMANLPEHPQWKTSPPPELTGAVRKILLRQIGVRNAENTL
                     YQNVLQQVSRNYADMTLADMTGDTLTESLFSTEQTVPGMFTRQAWEGQVREAIEQVVT
                     ARREEIDWVLSDRQQDTSADISPDTLRNRLTSRYFTDFAGSWLAFLNSIRWKKEDSLS
                     GILDQLTLMADARQSPLIALTDTIAWQVATGRENRGLSDSLAKSAQELFNGKEKTPQQ
                     SREGDVPAGPLDKTFAPLLRLLGDKSGGGGGDTQLNLQTYLTRVTRVRLKLQQVTNAP
                     DPQEMTQQLAQTVLQGKTVDLTDTRDYGRLIAASLGEEWSGFGQALFVRPVEQSWRQV
                     LTPAADSLNRQWQRAIVSHWNQDFAGRYPFKASQNDASLPLLAQYLRDDGRINQFIAA
                     NLSGVLKREGRYWVADAMNTQGLTVNPDFIRALNRLRDVADTAFASGDAGIHFELRAK
                     PARDVMKTHLVIDGQELEYFNQKERWQRFNWPDEQWQPGASLSWTSTQAMERILADYR
                     GSWSLIRLLERAQVTPVDSSTFKVVWKAQDGLPLNYLLRVEQGKGPLALLELKNFRLP
                     GQVFLTGRSMKDAEEYGEDADE"
     gene            4638..6248
                     /gene="tssA"
                     /locus_tag="NBI74_004056"
     CDS             4638..6248
                     /gene="tssA"
                     /locus_tag="NBI74_004056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001299109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system protein TssA"
                     /protein_id="HCD1934310.1"
                     /translation="MSELLQKLTRSCFADRDALDVARTQAALWQTWLLPVTADTPVGE
                     DPGYHDDFLRIRDEMNKLSGADTGLICQLAESLLLTQAKDVRIATYYIWARLHRDGER
                     GLAEGLALLAGLVERFGTQLLPSRPASRKMALEWLAGEKMLDSLARYPEVAKEDFANI
                     VAALNQLSVSFTAWPEDQHSPSLMPLINALESRLAQSGGMNAVVPQNSSGVPAPSSPV
                     DAPQVQTITSGRDLLDQAKVLARYLNEQPQGWLSAHRLMKTLRWDTVHELPPDVDGKT
                     RLAPPRTESRNQLKRLYAQQNWTELLEQADLMFSTGVSHFWLDIQWYLHQALAKAGAP
                     WDRWTAVIRQDLALLLERLPGLENLAWNDGTPFADEVTRNWIAQQVMMREDGAWLAGK
                     AAVPTDDATNDVLALEPEALEMADSQGVEAALGWIQTRPGITTARQRLLLRLLMARVA
                     EQYGKNEMALLLLEELDTAAQGITLTQWEPELLFEVKARQLKLLRLRAHRYADKALLN
                     RKMEILLGTLVAIDPVRAAVLCDTQHKD"
     gene            6254..7672
                     /locus_tag="NBI74_004057"
     CDS             6254..7672
                     /locus_tag="NBI74_004057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001402492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAAR domain-containing protein"
                     /protein_id="HCD1934311.1"
                     /translation="MGTGYFLVRGDKTTCGGKIIEGADDHTIMGIPQARDMDRVTCGR
                     YPGMFIIVGGVPETDIHGRLMAGTLDSQSSCPCKARFIASMMDDTYETDDGESEAEQH
                     AQSAKKDLTSGSDSSSDDVKLDYRIKLSGNKILTPLNIPDYKEMINGGSTKNTEKIDF
                     TITNKGDEAEGLSLEVLDGNEVIYSERQTGKYFTKGEHAWQWDGYSNQGILDTTKLKS
                     KSLLVRLIALCGDMMIKVDYPLKTSANEEDWVDIEVNKNNKTVNIIWRLAVSDGGIKG
                     SNPKLSPVPYNDLVDLTKNGVEFYWSRNGSRGGGIGESIVTAIGVFKVNVKAEINITP
                     SMRTFSLISSLDPDFQASVSLSGFEKIYYNYGDSYKDIQDELQALLDANNRYKWDSAH
                     EMGHKVLDEYGEGSSPDYSWTHKGTSTLMQKTIPGNVMPAQGEIDVMKYGKYRPDMYT
                     RLVAADEDVQGLIWLSRIKFDD"
     gene            7665..8087
                     /locus_tag="NBI74_004058"
     CDS             7665..8087
                     /locus_tag="NBI74_004058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1934312.1"
                     /translation="MTRVVIALLFLAGCTSQMKYSNVVRIRNVCSSDINVFIPEYVFG
                     GNVIKQHHIYLPVNGIDVLSYFYTENINDGFQYEEAFTVNKDSSGNFDYPFVILSSGR
                     EKKFGKNDLIELSRDISTKDDKKHGVVKYMIDSQNICP"
BASE COUNT         2027 a         2152 c         2424 g         1831 t
ORIGIN      
        1 attaacttct ggtcgataat gcaaaataaa gggatgtact gatgccagtc cgacttaata
       61 ccattcccga cgcagaaccc tatcccgtac cgccagcccg aattaagtgg ctgacagctt
      121 tattactgat gctgtctgtc ggtgtggccc tgacttcatt attcgccagc gatgaactgg
      181 caaaaaacgg cccgtatttc tgggggctgg cctgtggtgt gcctgccttt atctggtcac
      241 tggttgcttc agttcgctgg ctgtttttta tcacgcagta cattcggact gatgcctgga
      301 ataagcgccg ggaagaggtg attttgcagg aaacgcgccg gggacgacgg gcgttgcaga
      361 tactcagttt ttccgtacaa accgcgctga atggtgattc cgttgcagaa actataacag
      421 catttctggc acgtcagcag gtgttgaata cctacgctga cttgcgcgga gaagatactg
      481 tgcgtcgcag cgtcatccct gtattaacca ataaaccggt taccggacgt ctatccgaag
      541 ttttttcccg tctgttcagc gatatcaggc tgcaactgac gcggctaccc gatgattttc
      601 atatcaacgt actactggaa attgatgcgc cgctttcagg ggcctccgtg agaactatct
      661 ggcaggacga atggcaaaaa gccggactcc cggaagcccg tctgttttca gccccggagc
      721 cggggctggc cgccgtggac gactggctgg acaaccacat tcaggaaaag gccgtactgc
      781 tggtgatttc cgtgcgtctg gaaccgaaga acccggaacg gacggcagaa tccgccaccg
      841 cgctgttgct ggcaaaccgg ttgacgcaaa ccgcgctgac gccgctggcc ctgctgcatc
      901 gtcctgaatg tatcactgat acagagatga tggcgtcagg cattgcacag gcgctggact
      961 ggatgccggt gcagccagat gccatatccg gtatgtggac tgccgaactg gacagggaac
     1021 agcgtgccgc gttgttaagc ctgaaccagc cgtttgctca ggaagccctg atgtacgagc
     1081 tggatgcctt tctggggcgc tccggaccgg ctgcgccgtg gctgtccgtc gctgtggcaa
     1141 cgctggcggc aatacaatca caacaccccc aactgacgct cagcggcgtt cagggcgggc
     1201 attattcatg ggcgaccgtg gtgtcgccat tcgtttcccc tcaggaggca tcatagtggt
     1261 tccgggatta aaccgtctcc ggccccgttc ggtttcaggg cctgccatcg tcatgaccgt
     1321 ggctgttgtg gttgcgcttt gcacgtttat tcaccgtaaa ccggcattgc tgggcgttga
     1381 accgggtagc tcacagcaaa tgtactggat ggctggcgtg attgccgcag gcgtggtctt
     1441 tctgattatc atgggggttt atcatctgct gaaccgccac gctgccgtgc agaactttga
     1501 actccgcaga cagcttgccg ccggagatga ctatcgtgcg gtgacgacaa agaaagccga
     1561 cagccgtcat ccggcaatgg tggaactcgc cgccttcctg cgcgaccgct acggcccatt
     1621 ctggcggcgt aaaatccgcc tgctgctggt gaccggcgag cctgaacagg cagaggccat
     1681 cgcgccgggg ctgaccgggc aacactggct ggagggcgac cacacggtgc tgatatacgg
     1741 cggcaggcca tcagcggagc ctgatgtcgc gctactgacc gccttaaaaa aactgcgccg
     1801 cagccgtccg ctggacggca tcatctggcc gctgacagaa gaacagagcc gccagacagc
     1861 gcaactcgac aaaggctggc gcgaactgat aaacggcggt aagcgactcg gttttcaggc
     1921 cccattctat ttgtggcagg tctgtgacaa cggtgattat cagtccgggc gcgtcctgca
     1981 aagcgtcggc tgcctgctgc cggaaggctg taccccggaa caactggctg caatgctgga
     2041 agcacagacc ctaccgctga cggaacaggg catgtcgcag ctgctgaccg ataaccgcca
     2101 tgattttctg ctgcgtctgg cccataccct tgcagagcgg ggtatcaccc actggcagac
     2161 cgtcctgaaa ccgctgctgg caggcggcgc attttcttcc ctgcgcctgc gcggcctgat
     2221 gttcagcccg ccgcttgccg ccgtaccgga ggccgcacct catgcgtggc tgccgtcgcc
     2281 ggtctgggca ggcatcaccg gcgataacgc gcggggccgc gcggtgggct ttccgtggct
     2341 gcgtaccgca ctgatgtccg ctgtctgcgt gctggcgata gggggggccg gaatgacgac
     2401 ctcgttcttc gccaaccgcg cacttgttca ggaaaccagc atccagacgg cgcgggcgct
     2461 cgatacccgc ctgccgctgg cagaacaact ggtggcgctg cataccctgc agggcgaact
     2521 ggaacgcctg caataccgta tccgcgaagg tgcgccgtgg tatcagcgtt ttggccctga
     2581 acgtaaccag caactgctcg ccgccgcctt tcccggctat gcgcaggcgg caaaccggct
     2641 ggtgcgcgac gtggccgttg accatctgca acagcaactg aacgcctttg tcgccctgcc
     2701 gcccaacagt cctcagcgta ccgccaccgg tgaacaacgc tataagcagc ttaaggcatt
     2761 gctgatgact tcccgcccgg aaaaggccga cgctgccttt ttcagtacca cgctgatggc
     2821 agacggcctg cgctatgaaa atatcccgga aggcgtgcgg cagagcgtgt tgccgtcact
     2881 gctgaccttc tggatggcga acctgccgga acacccgcag tggaaaacct caccgccacc
     2941 ggaactgacc ggcgcggtgc gtaaaatcct gctgcgccag attggtgtgc gtaatgccga
     3001 aaacaccctc taccagaacg tgctgcaaca ggtgtcccgc aactacgccg atatgacgct
     3061 ggcggacatg accggggata ccctcactga atctcttttc agtacggaac agacggtgcc
     3121 ggggatgttc acccgtcagg catgggaagg acaggtcagg gaagctatcg agcaggtggt
     3181 gacggcgcgg cgcgaggaaa tcgactgggt actcagcgac cgccagcagg atacctctgc
     3241 tgatatctcg ccggatacgc tgcgtaaccg cctcacctca cgctacttta ccgactttgc
     3301 cggaagctgg ctggcgtttc tcaacagcat tcgctggaaa aaggaagact cgctctccgg
     3361 cattctcgac cagctgacgc tgatggccga tgcccgtcag tcgccactga ttgcactgac
     3421 agacactatt gcgtggcagg tggcaacagg cagggaaaat cgtggcctgt cagactcgct
     3481 ggcgaaatcg gcacaggaac tgtttaacgg caaggagaaa acgccgcagc aatcccgtga
     3541 aggcgacgtg cctgccgggc cgctggataa aaccttcgcg ccgctgctgc ggttgctggg
     3601 cgataagtca ggaggcggtg gtggtgatac gcaactcaac ctgcaaacgt atctgacccg
     3661 cgtcacccgc gtacgcctca aactgcaaca ggtgaccaac gcccccgacc cgcaggagat
     3721 gacccagcaa ctggcgcaga cggttttaca gggcaaaacc gttgacctca ccgacacccg
     3781 cgactacggg cggttaatcg ccgccagtct gggcgaagaa tggagtggct tcggtcaggc
     3841 gctgttcgtc cgcccggtgg aacagtcgtg gcggcaggtg ctgacgcccg cagcggacag
     3901 cctgaaccgc cagtggcaac gggcgattgt cagtcactgg aatcaggact tcgctggccg
     3961 ctatccgttc aaagcctcac agaacgatgc ctctcttccc ttgctggcgc agtacctgcg
     4021 cgatgacgga cgcatcaacc agtttatcgc cgccaacctt tccggtgttc tgaaacgtga
     4081 aggccgctac tgggtggctg acgccatgaa cacgcagggg ctgacggtca acccggactt
     4141 tatccgcgcc ctgaaccgcc tgcgcgatgt ggccgatacc gcctttgcca gcggcgatgc
     4201 cggaatacat ttcgaactgc gggcaaaacc ggcgcgtgac gtgatgaaaa cgcatctggt
     4261 gattgacggg caggaactgg agtatttcaa ccagaaagaa cgctggcagc gttttaactg
     4321 gccggatgaa cagtggcaac ccggcgcatc gctgagctgg accagcacac aggcgatgga
     4381 acgcatactg gcggattacc ggggaagctg gagccttatt cgcctgctgg aacgggcgca
     4441 ggtaacaccg gtcgacagca gcacctttaa agtggtgtgg aaagcgcagg acggcctgcc
     4501 actgaattac ctgctgcggg ttgaacaggg taaagggccg ctggcgctgc tggaactgaa
     4561 aaacttccgc ctgccgggac aggtatttct gaccggcagg agtatgaagg atgcggaaga
     4621 gtatggggaa gacgccgatg agtgaactgt tgcaaaagct gacccgtagc tgttttgccg
     4681 accgggatgc actggatgtg gcgcgaacac aggcggcgct gtggcagaca tggctgctgc
     4741 cggtgacggc agacacgccg gtcggggaag accccggcta ccacgacgat ttcctgcgta
     4801 tccgcgacga aatgaacaaa ctgtccggtg ccgataccgg ccttatctgc cagcttgccg
     4861 aatccctgct gctgacgcag gccaaagacg tgcgcatcgc cacttactac atctgggcgc
     4921 gactgcaccg cgacggtgag cgcggactgg ctgaaggact ggcgctgtta gccgggctgg
     4981 tggaacgctt cggtacgcaa ctgctgccct cgcgaccggc aagccgaaaa atggcgctgg
     5041 agtggctggc gggggaaaag atgctcgaca gccttgcccg ctacccggag gtggcaaaag
     5101 aggattttgc gaacattgtt gccgctctta atcaactgtc ggtttccttc actgcatggc
     5161 ccgaagacca gcattccccc tccctgatgc cgctgattaa cgcgctggaa tcacgtctgg
     5221 cgcagtcggg cgggatgaac gcggtggtgc cgcaaaacag cagcggcgtt ccggcaccgt
     5281 cttccccggt ggatgcgccg caggtacaga ccatcacctc cgggcgcgac ctactcgacc
     5341 aggcgaaagt gctggcccgt tatctgaacg aacagccgca gggctggctt tcagcacacc
     5401 ggctgatgaa aaccctgcgc tgggacacgg tgcatgaact gccgccggac gtggacggca
     5461 aaacccggct ggccccgccg cgcaccgaat cacgcaacca gcttaagcgg ctgtatgccc
     5521 agcagaactg gacggaactg ctggagcagg ccgacctgat gttcagcacc ggtgtcagcc
     5581 atttctggct ggatatccag tggtatctgc atcaggcgct ggcgaaggcg ggcgcaccgt
     5641 gggacagatg gacagcggtt atccgtcagg acctggcgct gctgctggag cgcctgccgg
     5701 ggctggaaaa tctggcatgg aacgacggca cgccgtttgc cgatgaggtg acgcgcaact
     5761 ggatagcgca gcaggtcatg atgcgggaag acggcgcatg gcttgcgggg aaagccgctg
     5821 tgccgacaga cgacgccaca aatgacgtac tggcgctgga gccggaagca ctggagatgg
     5881 ctgacagtca gggcgtggaa gcggcgctgg gctggataca gacccgtccg ggcatcacca
     5941 cggcacgaca gcgcctgtta ttgcgactgc tgatggcacg ggtggcggaa cagtacggta
     6001 aaaacgagat ggcgctcctg ctgctggaag aactggatac cgccgcacag ggaataacgc
     6061 tgacacagtg ggagccagag ctgctgtttg aagtaaaagc ccgacaactg aaactgctgc
     6121 gtctgcgggc gcaccgctat gcggataaag ccctgctgaa ccgaaaaatg gaaatcctgc
     6181 tgggaacgct ggtggcaatc gatccggtac gggcagcggt attgtgtgat acgcaacata
     6241 aggactgatg gcaatgggaa caggctactt tctggtcagg ggggataaga ccacctgtgg
     6301 tggaaaaatt attgaagggg cggacgacca tacgatcatg ggtatcccgc aggcacggga
     6361 tatggaccgg gtcacctgcg gcagatatcc gggaatgttt attattgtgg gcggcgttcc
     6421 tgaaacagat attcacggca ggctgatggc ggggacgctg gacagtcaga gcagttgccc
     6481 gtgtaaagca cggtttattg cgtcaatgat ggatgatacg tatgagacgg atgacgggga
     6541 aagtgaagcg gaacaacatg cgcagtcggc gaaaaaggat ctgacttctg gttctgattc
     6601 atcctctgat gatgtaaaac tggattacag aattaaatta tcaggtaata agatactgac
     6661 gccactgaat atacctgatt ataaagaaat gataaacggt ggtagcacga agaatacaga
     6721 aaaaatagat tttaccatca caaataaagg cgatgaggct gaaggacttt ccctggaagt
     6781 gcttgatggc aatgaagtga tttatagtga acggcagacc ggtaaatatt ttactaaggg
     6841 agagcatgcc tggcaatggg atggctacag taatcaaggc atactggata caactaagct
     6901 aaaaagtaaa tcactactcg taagactgat tgctttatgt ggtgatatga tgataaaagt
     6961 tgattatcca ttgaaaactt ctgcgaatga ggaagattgg gtagatatag aagttaacaa
     7021 aaacaataag acagtaaata taatatggcg actggcagtt agtgatggag ggataaaagg
     7081 aagtaatcct aagttatccc cagttcctta taacgacttg gttgacctga ccaaaaatgg
     7141 agttgaattt tattggtctc gaaatggaag tcgcggcggg gggattggtg aaagtattgt
     7201 tacagcgatt ggtgttttta aagtaaatgt aaaggctgag ataaatatta ctccatccat
     7261 gcgaactttt tctttgatat catcattaga tcctgacttt caggcatccg ttagtctatc
     7321 aggatttgaa aaaatatatt ataactacgg tgatagttat aaggatattc aagatgaatt
     7381 acaggcactt cttgacgcta ataacagata taaatgggat agtgctcatg aaatggggca
     7441 taaagtactg gatgaatatg gagagggttc tagccctgat tattcgtgga ctcataaagg
     7501 aacatctact cttatgcaaa aaacaatacc agggaatgtt atgcccgctc agggagagat
     7561 tgatgtaatg aaatatggaa aatatcgtcc tgacatgtat actcgattgg ttgcagcaga
     7621 tgaagatgtt caggggttga tctggttaag caggataaaa tttgatgact agagtagtta
     7681 ttgctttatt atttcttgcc ggatgtactt cgcaaatgaa gtattccaat gtcgtcagga
     7741 ttaggaatgt atgttcctca gatattaatg tgtttattcc tgagtatgtt tttggaggta
     7801 atgttattaa gcaacatcat atttacttac ctgttaatgg gattgatgtt ttgtcttatt
     7861 tttatactga aaacatcaat gatggtttcc aatatgaaga agcattcacc gtaaataaag
     7921 atagctcggg taattttgac tatccgtttg ttatcttatc atcaggacgt gaaaaaaaat
     7981 ttgggaaaaa cgatctaatt gaattatcca gggacatttc aacaaaagac gataagaagc
     8041 atggggttgt aaagtacatg atagatagtc agaatatttg cccatagtat gattcatatc
     8101 ataatcaact gaaaaattga cattattaat catttgtaat tttttgatac taccaagtga
     8161 aatttgatga ttagattaat attctctttt ttattatgca cactgtgtgc tggatgttta
     8221 tctcaaccat tctattcttg ttccattgtc atggtaaata catgcaaagt atctattagc
     8281 atttcgacag ttaataattc aacacattat gatggtaaag taagagataa tttccatctt
     8341 gatgtcggag aacgaaagcc aattgtatac tttatttaca caggtagaga gtcagatgtt
     8401 tttaataaaa accctgtgaa tattgtgtct gaac
//
LOCUS       DAHHTG010000167         8394 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.170, whole genome shotgun sequence.
ACCESSION   DAHHTG010000167 DAHHTG010000000
VERSION     DAHHTG010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8394)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8394)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8394
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.170"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            <1..2832
                     /gene="evgS"
                     /locus_tag="NBI74_004059"
     CDS             <1..2832
                     /gene="evgS"
                     /locus_tag="NBI74_004059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system histidine kinase EvgS"
                     /protein_id="HCD1934313.1"
                     /translation="VLNRFVDALTNEVRYEVSQNWLDTGNLAFLNKPLELTEHEKQWI
                     KQHPDLKVLENPYSPPYSMTDETGSVRGVMGDILNIITLQTGLNFSPITVSHNIHAGT
                     QLNPGGWDILPAAIYSEDRENNVSFAEVFITTPYVFVMQKAPDSEQTLKKGMKVAIPY
                     YYELHSQLKEMYPEVEWIKVDNASAAFHKVKEGELDALVATQLNSRYMIDHYYPNELY
                     HFLIPGVQNASLSFAFPRGEPELKDIINKALNAIPPSEVLRLTEKWIKMPNVTIDTWD
                     LYSEQFYIVTTLSVLLVGSSLLWGFYLLRSVRRRKVIQGDLENQISFRKALSDSLPNP
                     TYVVNWQGNVISHNSAFEHYFTANYYKNAMLPLENSESPFKDVFSNTHEVTAETKENR
                     KIYTQVFEIDNGIEKRCINHWHTLCNLPASEHAVYICGWQDITETRDLIHALEVERNK
                     AINATVAKSQFLATMSHEIRTPISSIMGFLELLSGSGLSKEQRVEAISLAYATGQSLL
                     GLIGEILDVDKIESGNYQLQPQWVDIPTLVQNTCHSFAAIAASKSIALSCSSTLPERY
                     LVKIDPQAFKQVLSNLLSNALKFTTEGAVKITTSLGHIDDNHAVIKMTIMDSGSGLSQ
                     EEQQQLFKRYSQTSAGRQQTGSGLGLMICKELIKNMQGDLSLESHPGIGTIFTITIPV
                     EITQQVAAVEAKAEQPITLPEKLSILIADDHPTNRLLLKRQLNLLGYDVDEATDGVQA
                     LHKISMQHYDLLITDVNMPNMDGFELTRKLREQNSSLPIWGLTANAQANEREKGLNCG
                     MNLCLFKPLTLDVLKTHLSQLHQVAHIAPQYRHLDIEALKNNTANDLQLMQEILMTFQ
                     HETHKDLPAAFHALEAGDNRTFHQCIHRIHGAANILNLQKLINISHQLEITPVSDDSK
                     PEILQLLNSVKEHIAELDQEITVFCQQND"
     gene            complement(2888..4033)
                     /gene="yfdE"
                     /locus_tag="NBI74_004060"
     CDS             complement(2888..4033)
                     /gene="yfdE"
                     /locus_tag="NBI74_004060"
                     /EC_number="2.8.3.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416872.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA:oxalate CoA-transferase"
                     /protein_id="HCD1934314.1"
                     /translation="MTNNECKGPFEGLLVIDMTHVLNGPFGTQLLCNMGARVIKVEPP
                     GHGDDTRTFGPYVDGQSLYYSFINHGKESVVLDLKNDHDKSIFINMLKQADVLAENFR
                     PGTMEKLGFSWERLQEINPRLIYASSSGFGHTGPLKDAPAYDTIIQAMSGIMMETGYP
                     DAPPVRVGTSLADLCGGVYLFSGIVSALYGREKSQRGAHVDIAMFDATLSFLEHGLMA
                     YIATGKSPQRLGNRHPYMAPFDVFDTQDKPITICCGNDKLFSALCQALELTELVNDPR
                     FSSNILRVHNQAILKEYIERTLKTQVAEVWLARIHEVGVPVAPLLSVAEAINLPQTQA
                     RNMLIEAGGIMMPGNPIKISGCADPHVMPGAATLDQHGEQIRQEFSS"
     gene            complement(4107..5051)
                     /gene="yfdV"
                     /locus_tag="NBI74_004061"
     CDS             complement(4107..5051)
                     /gene="yfdV"
                     /locus_tag="NBI74_004061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter YfdV"
                     /protein_id="HCD1934315.1"
                     /translation="MLTFFIGDLLPIIVIMLLGYFSGRRETFSEDQARAFNKLVLNYA
                     LPAALFVSITRANREMIFADTRLTLVSLVVIVGCFFFSWFGCYKFFKRTHAEAAVCAL
                     IAGSPTIGFLGFAVLDPIYGDSVSTGLVVAIISIIVNAITIPIGLYLLNPSSGADGKK
                     NSNLSALISAAKEPVVWAPVLATILVLVGVKIPAAWDPTFNLIAKANSGVAVFAAGLT
                     LAAHKFEFSAEIAYNTFLKLILMPLALLLVGMACHLNSEHLQMMVLAGALPPAFSGII
                     IASRFNVYTRTGTASLAVSVLGFVVTAPLWIYVSRLVS"
     gene            complement(5121..6815)
                     /gene="oxc"
                     /locus_tag="NBI74_004062"
     CDS             complement(5121..6815)
                     /gene="oxc"
                     /locus_tag="NBI74_004062"
                     /EC_number="4.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxalyl-CoA decarboxylase"
                     /protein_id="HCD1934316.1"
                     /translation="MSDQLQMTDGMHIIVEALKQNNIDTIYGVVGIPVTDMARHAQAE
                     GIRYIGFRHEQSAGYAAAASGFLTQKPGICLTVSAPGFLNGLTALANATVNGFPMIMI
                     SGSSDRAIVDLQQGDYEELDQMNAAKPYAKAAFRVNQPQDLGIALARAIRVSVSGRPG
                     GVYLDLPANVLAATMEKDEALTTIVKVENPSPALLPCPKSVTSAISLLAKAERPLIIL
                     GKGAAYSQSDEQLREFIESAQIPFLPMSMAKGILEDTHPLSAAAARSFALANADVVML
                     VGARLNWLLAHGKKGWAADTQFIQLDIEPQEIDSNRPIAVPVVGDIASSMQGMLAELK
                     QNTFTTPLVWRDILNIHKQQNAQKMHEKLSTDTQPLNYFNALSAVRDVLRENQDIYLV
                     NEGANTLDNARNIIDMYKPRRRLDCGTWGVMGIGMGYAIGASVTSGSPVVAIEGDSAF
                     GFSGMEIETICRYNLPVTIVIFNNGGIYRGDGVDLSGAGAPSPTDLLHHARYDKLMDA
                     FRGVGYNVTTTDELRHALTTGIQSRKPTIINVVIDPAAGTESGHITKLNPKQVAGN"
     gene            complement(6869..8119)
                     /gene="frc"
                     /locus_tag="NBI74_004063"
     CDS             complement(6869..8119)
                     /gene="frc"
                     /locus_tag="NBI74_004063"
                     /EC_number="2.8.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formyl-CoA transferase"
                     /protein_id="HCD1934317.1"
                     /translation="MSTPLQGIKVLDFTGVQSGPSCTQMLAWFGADVIKIERPGVGDV
                     TRHQLRDIPDIDALYFTMLNSNKRSIELNTKTAEGKEVMEKLIREADILVENFHPGAI
                     DHMGFTWEHIQEINPRLIFGSIKGFDECSPYVNVKAYENVAQAAGGAASTTGFWDGPP
                     LVSAAALGDSNTGMHLLIGLLAALLHREKTGRGQRVTMSMQDAVLNLCRVKLRDQQRL
                     DKLGYLEEYPQYPNGTFGDAVPRGGNAGGGGQPGWILKCKGWETDPNAYIYFTIQEQN
                     WENTCKAIGKPEWITDPAYSTAHARQPHIFDIFAEIEKYTVTIDKHEAVAYLTQFDIP
                     CAPVLSMKEISLDPSLRQSGSVVEVEQPLRGKYLTVGCPMKFSAFTPDIKAAPLLGEH
                     TAAVLQELGYSDDEIAAMKQNHAI"
BASE COUNT         2458 a         1943 c         1750 g         2243 t
ORIGIN      
        1 gtactcaatc gatttgttga tgctttaaca aatgaagttc gctatgaagt atcacaaaat
       61 tggcttgata caggaaacct ggcctttctg aacaaaccat tagaactcac tgaacatgaa
      121 aaacagtgga ttaagcagca tcccgattta aaggtgctgg aaaatcctta ctcgccaccc
      181 tattctatga cagatgaaac tggctcagtt cggggcgtga tgggtgatat tcttaatatt
      241 attaccttgc aaacaggttt aaatttttct ccgatcaccg tttcacacaa tattcatgct
      301 gggacacagc ttaaccccgg tggatgggat atattgcccg ccgctattta tagtgaagat
      361 cgagaaaata atgtttcatt tgctgaagtc ttcataacaa cgccttacgt ttttgtcatg
      421 caaaaagcgc ctgacagtga acaaacatta aaaaaaggaa tgaaagttgc cattccatat
      481 tattatgagc ttcattcgca attaaaagag atgtatccgg aggtagagtg gataaaagtc
      541 gataacgcca gcgctgcatt tcacaaggtc aaggaaggtg aacttgatgc tctggtcgcg
      601 acacagttaa attcgcgtta catgatcgac cattactatc ctaatgaact ttatcatttt
      661 cttattcccg gcgttcagaa tgcatcactt tcgttcgctt ttcctcgcgg agaaccggaa
      721 cttaaggata ttattaataa agcactgaat gcaattcccc caagcgaagt tctgcgcctg
      781 accgaaaaat ggattaaaat gcccaatgtg accattgaca catgggacct ctatagcgag
      841 caattttata ttgttacgac attatccgtt ttattagttg gcagtagcct tttatgggga
      901 ttctacctgt tacgctcagt tcgtcgtcgt aaagttattc agggtgattt agaaaaccaa
      961 atatcattcc ggaaagcgct ctcggattcc ttacctaatc caacttatgt tgtaaactgg
     1021 caaggtaatg tcattagtca caatagtgca tttgaacatt atttcactgc taattactat
     1081 aaaaatgcaa tgttaccatt agaaaacagt gaatccccct ttaaagatgt tttttctaat
     1141 acgcatgaag tcacagcaga aacgaaagaa aaccgaaaaa tatacacaca ggtatttgaa
     1201 attgataatg gcatcgagaa aagatgcatt aatcactggc ataccttatg taatctgcca
     1261 gcaagcgaac atgctgttta tatttgtggt tggcaagata ttaccgagac gcgtgattta
     1321 attcatgcac tcgaagtaga gagaaataaa gcgatcaatg caactgtcgc aaaaagccag
     1381 tttctggcaa caatgagtca cgaaataaga acaccaataa gctccattat gggcttcctg
     1441 gaacttctgt cgggttctgg tcttagcaag gaacaacggg tggaggcgat ttcacttgcc
     1501 tacgccaccg gacaatcact cctcggctta attggtgaaa tccttgatgt cgacaaaatt
     1561 gaatcgggta actatcaact tcaaccacaa tgggtcgata tccctacttt agtccagaac
     1621 acttgtcact ctttcgctgc gattgctgca agcaaatcga tcgcattaag ttgcagcagt
     1681 acgcttcctg aacgctacct ggttaagatc gaccctcagg cgtttaagca ggtcttatct
     1741 aatttactga gtaatgctct caaatttact accgaggggg cagtaaaaat tacgacctcc
     1801 ctgggtcaca ttgatgacaa ccacgctgtt atcaaaatga cgattatgga ttctggaagt
     1861 ggattatcgc aggaagaaca acaacaactg tttaaacgct atagccaaac aagtgcaggt
     1921 cgtcagcaaa caggttctgg tttaggctta atgatctgca aagaattaat taaaaacatg
     1981 cagggcgatt tgtcattaga aagtcatcca ggcataggaa cgatatttac gatcacaatc
     2041 ccggtagaaa ttacccaaca agtggcggct gtcgaggcaa aagcagaaca acctataaca
     2101 ctacctgaaa agttgagcat attaatcgcg gatgatcatc cgaccaacag gctattactc
     2161 aaacgccagc taaatctatt aggatatgat gttgatgaag ccactgatgg tgtgcaagcg
     2221 ctacacaaaa tcagtatgca acattatgat ctgcttatta ctgacgttaa tatgccgaat
     2281 atggatggtt ttgagttgac tcgcaaactc cgtgagcaaa attcttcctt acccatctgg
     2341 gggcttacag ccaacgcaca ggctaacgaa cgtgaaaaag ggttaaattg cggcatgaac
     2401 ttatgtttgt tcaaaccgtt gactctggat gtactgaaaa cacatttaag tcagttacac
     2461 caggttgcgc atattgcacc tcagtatcgc caccttgata tcgaggccct gaagaataat
     2521 acggcgaatg atctacaact gatgcaggag attctcatga ctttccagca tgaaacgcat
     2581 aaagatctac ccgctgcgtt tcatgcacta gaagctggcg ataacagaac tttccatcag
     2641 tgtattcatc gcatccacgg tgcggctaac atcctgaatt tgcaaaagtt gattaatatt
     2701 agccaccagt tagaaataac acctgtttca gatgacagta agcctgaaat tcttcagttg
     2761 ctgaactctg taaaagaaca cattgcagag ctggaccagg agattactgt tttctgtcag
     2821 caaaatgact aaatagcggc tcccacaatg ttcaaatgtg ggagctattt accagcacat
     2881 cttacattta tgatgagaac tcctggcgaa tttgttcccc atgctggtcg agcgttgccg
     2941 ctcccggcat aacatgcggg tccgcgcagc cgctgatttt tatcggatta cccggcatca
     3001 ttattccccc ggcttcaatc aacatatttc tcgcctgagt ttgtggtaaa ttaatggcct
     3061 cagccacact taataacggc gcgacgggca caccaacttc atgtattctg gctaaccaaa
     3121 cttcagctac ctgcgttttt aacgtccgct caatatattc tttaagaata gcctggttat
     3181 gtacgcgtaa aatattgctg ctaaatcggg gatcattaac cagttccgta agctccagtg
     3241 cctggcataa cgcagaaaaa agcttgtcat taccacaaca aatcgtaatc ggcttatcct
     3301 gagtatcgaa aacatcaaaa ggtgccatgt agggatggcg atttcccagg cgttgtggtg
     3361 acttcccagt cgcgatatat gccatcagtc catgctccag aaaactcaga gtggcatcaa
     3421 acatcgctat atcgacatgc gcccctctct ggctcttttc gcggccataa agtgcactca
     3481 ctattccact gaataaataa acaccgccac agagatccgc aagagaggta ccaacgcgca
     3541 ctggcggagc atcagggtat cctgtttcca tcattatccc gctcattgcc tgaatgatgg
     3601 tatcgtaggc aggagcatct tttagcggac cggtatgtcc gaaacctgac gatgaagcat
     3661 atataaggcg cgggttgatt tcttgtaacc tttcccatga aaacccgagt ttttccattg
     3721 tacctgggcg aaaattctca gctaatacat cagcttgttt aagcatattt ataaatatac
     3781 ttttatcgtg atcattcttt aaatcaagaa ccacactctc tttgccatga ttaataaaac
     3841 tgtaatagag tgactgtcca tccacatagg gaccaaatgt gcgggtatca tcaccatgac
     3901 ccggtggctc aactttaatt acccttgcgc ccatattaca aagaagttga gttccgaaag
     3961 gcccattaag gacatgcgtc atatcgataa ctaataagcc ttcaaacggc cctttgcatt
     4021 cattatttgt catttgctta tgccttgaaa aaagccgaag gtgaaaagac acttcggcat
     4081 ttatgtaaaa tcaataacat gataaattat gaaaccagtc gactgacata aatccacaaa
     4141 ggagccgtga cgacaaaacc cagtacgctc acagccaatg acgctgtacc agtacgggtg
     4201 tagacattaa accggctggc aataatgatt ccggagaatg ccggcggtaa tgcgcctgcc
     4261 agtaccatca tctgcaaatg ttcgctattc aaatgacatg ccataccgac gagaagcagc
     4321 gccagtggca tcagaatcag cttcaggaaa gtgttataag caatttcggc actgaactcg
     4381 aatttatgtg cagccagagt caaccccgca gcgaataccg ctacccctga gttagccttc
     4441 gcaatcagat taaaggttgg gtcccatgct gccggaattt ttaccccaac cagcaccagg
     4501 atcgttgcca gaacaggtgc ccatactact ggctcctttg ccgcagaaat taatgcgctc
     4561 agattactat tcttcttacc atccgctcct gaggaaggat tcagcaaata cagaccaata
     4621 ggaatagtaa ttgcgttaac aataatagaa ataattgcta ccactaaacc tgttgatacg
     4681 gaatcaccat aaataggatc gagaactgca aaccccagga atccaatggt aggtgaacct
     4741 gcaattaatg cacatactgc tgcttctgca tgggtacgtt taaaaaattt gtagcaaccg
     4801 aaccaggaga aaaagaaaca tccgacaata accacaagtg atacaagggt caggcgagtg
     4861 tccgcaaaaa tcatttccct gtttgccctg gtaatagaaa caaatagagc cgcaggaaga
     4921 gcgtagttta ataccagttt attaaatgcc cgggcttgat cttctgaaaa agtttctcgt
     4981 ctgccgctaa aataacccaa cagcatgatt acgataatag gcaataaatc gccaataaaa
     5041 aatgttagca taatagtatt tccgataagg gtcccctcac catcgcataa taagttggca
     5101 agaggagacg atgtcagtat ttaattacca gcgacttgtt ttgggttaag tttggtaata
     5161 tggccacttt cagttcctgc tgctgggtcg atgaccacat taataatggt cggtttgcgc
     5221 gactgaattc cggtggttaa agcatgacga agttcatctg tcgtggtgac gttatagcca
     5281 acgccacgaa acgcatccat taatttgtca taccttgcat ggtgcaacag atccgttggc
     5341 gatggtgcac cagcgccgct gaggtcaaca ccgtctcctc tgtagatgcc gccattatta
     5401 aaaataacga tcgtcaccgg caggttatat cgacaaatcg tttcaatctc catcccactg
     5461 aaaccaaaag cactatcacc ttcaatagcg acaaccggag aaccagaggt cacgctagca
     5521 ccgatggcat agcccatacc gatgcccatg acaccccagg tgccacaatc cagacgacga
     5581 cgtggtttat acatatcaat aatatttcgt gcattatcca gggtatttgc cccttcatta
     5641 actaaataaa tatcctggtt ctcgcgcaaa acatcgcgca cggcactcaa tgcattaaag
     5701 taatttaatg gttgggtatc tgtacttaac ttttcatgca ttttttgtgc attttgctgc
     5761 ttgtggatat ttaaaatatc acgccacacc agtggagtcg taaatgtgtt ttgtttcaat
     5821 tctgccagca taccttgcat actggatgca atatcgccaa cgactggcac agcaatgggg
     5881 cggttgctgt caatttcctg cggttcaata tccagttgaa taaactgtgt atctgccgcc
     5941 catccttttt taccgtgtgc cagtaaccaa ttcagtcgtg caccaacaag catgacaacg
     6001 tcagcatttg ccagggcaaa cgaacgcgca gctgccgcag agagtggatg cgtatcttcg
     6061 aggatccctt tcgccataga cattggcagg aatggaatct gggcactttc aataaattca
     6121 cgaagctgtt catcagattg tgaatacgcc gcgcctttgc caagaataat taatggtcgt
     6181 tcagcttttg ctaaaagcga aattgcgcta gtgactgact tcgggcatgg caataatgct
     6241 ggcgacggat tttcaacttt aacaatcgtg gttaacgctt cgtctttttc catcgtcgcg
     6301 gccaggacat ttgctggcaa atcaagataa acaccgccag gacgacccga tactgagacc
     6361 cggatagcgc gtgccaatgc aatgccaaga tcctgcggct gattaacgcg aaatgctgct
     6421 ttggcatacg gttttgccgc attcatttgg tccagctctt cataatcacc ttgctggaga
     6481 tcgacgatcg cgcggtcgct ggagccgcta atcatgatca tcggaaaacc atttaccgtt
     6541 gcattggcca atgcggtcaa accgttgagg aatcctggcg cagacactgt caggcagatc
     6601 cctggttttt gggtaagaaa accgcttgct gcagcggcat aacctgccga ctgctcatga
     6661 cgaaaaccaa tataacgaat gccttccgcc tgggcatggc gcgccatatc cgtcacagga
     6721 atacctacaa caccataaat agtgtcaata ttattctgtt ttaatgcttc aacgatgata
     6781 tgcataccat ctgtcatttg aagttgatct gacatgatta ataacctcga aatttttata
     6841 aattgggagc cagaagcccc cgttaatatc agatggcgtg gttttgcttc attgcagcaa
     6901 tttcatcgtc gctataaccc agctcctgca atacagcagc ggtatgttca cctaatagcg
     6961 gcgcagcttt aatatccggc gtaaaggcag agaatttcat tggacaacca actgtcagat
     7021 attttccacg caacggttgt tccacttcga caacactgcc actttggcgc aaagagggat
     7081 caagtgaaat ttctttcata cttaaaaccg gtgcgcaagg aatatcaaac tgagtcaaat
     7141 aggccacagc ttcatgttta tcaatagtga cagtgtattt ttcgatttca gcaaaaatat
     7201 cgaaaatatg tggctgtcgg gcatgggctg tactgtatgc cggatcggta atccattctg
     7261 gtttgccgat ggctttacag gtgttttccc agttttgctc ctgaatagtg aaataaatat
     7321 aggcgttagg atcggtttcc cagcctttac atttcaggat ccagccaggc tgaccaccac
     7381 cacccgcatt accaccgcgg ggaactgcat caccaaatgt gccattcgga tactgcgggt
     7441 attcttccag ataacccaat ttatcgagac gctgctggtc gcgtaatttc acgcggcaaa
     7501 ggttcaacac ggcatcctgc attgacatgg tcactcgttg cccacgcccc gttttttcgc
     7561 gatgcagcaa agcagcaagt aaaccgatca gcaaatgcat tccggtgttg ctgtcaccca
     7621 acgctgcagc gcttaccagc ggcggaccgt cccaaaaacc tgtagtagat gccgcgccac
     7681 ccgctgcctg agcaacgttt tcataggctt ttacattcac ataaggcgaa cactcatcaa
     7741 accctttgat cgaaccaaaa atcagacgtg gattgatttc ttgaatatgc tcccaggtga
     7801 agcccatatg atcaatggcc cctggatgaa agttctcgac taagatatca gcttcgcgga
     7861 tcagcttttc cattacctct ttgccttccg ctgttttggt atttaattca atagaacgtt
     7921 tgttactgtt aagcatggtg aagtaaagcg catcgatatc aggaatatct cgcagctggt
     7981 ggcgcgttac gtcaccaacg ccgggacgtt caattttaat gacgtcagcg ccaaaccagg
     8041 ccagcatttg agtacaagat gggccagatt gcacaccggt gaaatcgaga actttaattc
     8101 cttgaagtgg agttgacata gcaataccta ttagttaatt aaatatgtgt taatttagcg
     8161 tttatttaag gcataacgcc tctataaata atctgcaaaa tatcgtcgta gctgcactcg
     8221 atatttttaa attcctgtat caagtgctga aaatcatttc atcgctactt ccaattcctt
     8281 tctgcatggt aagtagtgag gacgaaaaga agaatataat aaatattggt tcgcggtaat
     8341 cagagagagc cgcgacattt gtgatgttat tcttacaact gtaagaagtg tcag
//
LOCUS       DAHHTG010000168         8331 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.171, whole genome shotgun sequence.
ACCESSION   DAHHTG010000168 DAHHTG010000000
VERSION     DAHHTG010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8331)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8331)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8331
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.171"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            complement(118..1185)
                     /gene="purK"
                     /locus_tag="NBI74_004064"
     CDS             complement(118..1185)
                     /gene="purK"
                     /locus_tag="NBI74_004064"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HCD1934318.1"
                     /translation="MKQVCVLGNGQLGRMLRQAGEPLGIAVWPVGLDAEPAAVPFQQS
                     VITAEIERWPETALTRELARQPAFVNRDVFPIIADRLTQKQLFDKLHLPTAPWQLLAE
                     RSEWPAVFDRLGELAIVKRRTGGYDGRGQWRLRAAETEQLPAECYGECIVEQGINFSG
                     EVSLVGARGFDGSTVFYPLTHNLHQDGILRTSVAFPQANAQLQAQAEEMLSAIMQELG
                     YVGVMAMECFVTPQGLLINELAPRVHNSGHWTQNGASISQFELHLRAITDLPLPQPVV
                     NNPSVMINLIGSDLNYDWLKLPLVHLHWYDKEVRPGRKVGHLNLTDSDTSHLTATLEA
                     LIPLLPPEYASGVMWAQSKFS"
     gene            complement(1182..1691)
                     /gene="purE"
                     /locus_tag="NBI74_004065"
     CDS             complement(1182..1691)
                     /gene="purE"
                     /locus_tag="NBI74_004065"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HCD1934319.1"
                     /translation="MSSRNNPARVAIVMGSKSDWATMQFAAEIFEILNVPHHVEVVSA
                     HRTPDKLFSFAESAEENGYQVIIAGAGGAAHLPGMIAAKTLVPVLGVPVQSAALSGVD
                     SLYSIVQMPRGIPVGTLAIGKAGAANAALLAAQILATHDKELHQRLNDWRKAQTDEVL
                     ENPDPRGAA"
     gene            complement(1787..2116)
                     /locus_tag="NBI74_004066"
     CDS             complement(1787..2116)
                     /locus_tag="NBI74_004066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001188438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1934320.1"
                     /translation="MQRCNGFLISGIALTAGAPLCISWFGLVGFYFGALSLVAAAVIS
                     WIVYFWLIYLVLTRPHNQRFVERYILIVGICFLPWSIVLIWAFFASATRAHNQQAEQT
                     KNSTTDG"
     gene            complement(2227..2949)
                     /gene="lpxH"
                     /locus_tag="NBI74_004067"
     CDS             complement(2227..2949)
                     /gene="lpxH"
                     /locus_tag="NBI74_004067"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HCD1934321.1"
                     /translation="MATLFIADLHLCVEEPAITAGFLRFLAGEARKADALYILGDLFE
                     AWIGDDDPNPLHHQMAAAIKAVSDSGVPCYFIHGNRDFLLGKRFARESGMTLLPEEKV
                     LELYGRRVLIMHGDTLCTDDAGYQAFRAKVHKPWLQTLFLALPLFVRKRIAARMRANS
                     KEANSSKSLAIMDVNQNAVVSAMEKHQVQWLIHGHTHRPAVHELIANQQTAFRVVLGA
                     WHTEGSMVKVTADDVELIHFPF"
     gene            complement(2952..3446)
                     /gene="ppiB"
                     /locus_tag="NBI74_004068"
     CDS             complement(2952..3446)
                     /gene="ppiB"
                     /locus_tag="NBI74_004068"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017465360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase B"
                     /protein_id="HCD1934322.1"
                     /translation="MVTFHTNHGDIVIKTFDDKAPETVKNFLDYCREGFYNNTIFHRV
                     INGFMIQGGGFEPGMKQKATKDPIKNEANNGLKNTRGTLAMARTQAPHSATAQFFINV
                     VDNDFLNFSGESLQGWGYCVFAEVVEGMDVVDKIKGVATGRSGMHQDVPKEDVIIESV
                     TVSE"
     gene            3620..5005
                     /gene="cysS"
                     /locus_tag="NBI74_004069"
     CDS             3620..5005
                     /gene="cysS"
                     /locus_tag="NBI74_004069"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HCD1934323.1"
                     /translation="MLKIFNTLTRQKEEFKPIHAGEVGMYVCGITVYDLCHIGHGRTF
                     VAFDVVARYLRFLGYKLKYVRNITDIDDKIIKRANENGESFVALVDRMIAEMHKDFDA
                     LNILRPDMEPRATHHIAEIIELTEQLIAKGHAYVADNGDVMFDVPTDPTYGVLSRQDL
                     DQLQAGARVDVVDDKRNPMDFVLWKMSKEGEPSWPSPWGAGRPGWHIECSAMNCKQLG
                     NHFDIHGGGSDLMFPHHENEIAQSTCAHDGQYVNYWMHSGMVMVDREKMSKSLGNFFT
                     VRDVLKYYDAETVRYFLMSGHYRSQLNYSEENLKQARAALERLYTALRGTDKTVAPAG
                     GEAFEARFIEAMNDDFNTPEAYSVLFDMAREVNRLKAEDMAAANAMASHLRKLSAVLG
                     LLEQEPEAFLQSGAQADDSEVAEIEALIQQRLDARKAKDWAAADAARDRLNEMGIVLE
                     DGPQGTTWRRK"
     gene            complement(5041..5562)
                     /locus_tag="NBI74_004070"
     CDS             complement(5041..5562)
                     /locus_tag="NBI74_004070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HCD1934324.1"
                     /translation="MPTVITHAAVPLCIGLGLGSKAIPPRLLFAGIILAMLPDADVLS
                     FKFGVAYGNVFGHRGFTHSLVFAFVVPLLCVLIGRRWFRAGLIRCWLFLTVSLLSHSL
                     LDSVTTGGKWVGWLWPWSDERFFAPWQVIKVAPFALSRYTTPYGHQVIISELMWVWLP
                     GMLLMGMLWWRRR"
     gene            complement(5670..5882)
                     /gene="ybcJ"
                     /locus_tag="NBI74_004071"
     CDS             complement(5670..5882)
                     /gene="ybcJ"
                     /locus_tag="NBI74_004071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein YbcJ"
                     /protein_id="HCD1934325.1"
                     /translation="MATFSLGKHPHVELCDLLKLEGWSESGAQAKIAIAEGLVKVDGA
                     VETRKRCKIVAGQTVSFAGHSVQVVA"
     gene            complement(5884..6750)
                     /gene="folD"
                     /locus_tag="NBI74_004072"
     CDS             complement(5884..6750)
                     /gene="folD"
                     /locus_tag="NBI74_004072"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HCD1934326.1"
                     /translation="MAAKIIDGKTIAQQVRSEVAQKVQARIAAGLRAPGLAVVLVGSN
                     PASQIYVASKRKACEEVGFVSRSYDLPETTSEAELLELIDALNADNTIDGILVQLPLP
                     AGIDNVKVLERIHPDKDVDGFHPYNVGRLCQRAPRLRPCTPRGIVTLLERYNIDTFGL
                     NAVVIGASNIVGRPMSMELLLAGCTTTVTHRFTKNLRHHVENADLLIVAVGKPGFIPG
                     DWIKEGAIVIDVGINRLENGKVVGDVVFEDAAKRASYITPVPGGVGPMTVATLIENTL
                     QACVEYHDPQGE"
     gene            6791..6988
                     /locus_tag="NBI74_004073"
     CDS             6791..6988
                     /locus_tag="NBI74_004073"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1934327.1"
                     /translation="MSILSEAWRAVSFRFLKSKTFCTWLIHTYHDNRRWFAAIVSYIR
                     CGRTLVQQLVQKPLTQQGLTV"
     gene            7021..7097
                     /locus_tag="NBI74_004074"
     tRNA            7021..7097
                     /locus_tag="NBI74_004074"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7055..7057,aa:Arg,seq:tct)
     gene            complement(7105..7713)
                     /locus_tag="NBI74_004075"
                     /pseudo
     CDS             complement(7105..7713)
                     /locus_tag="NBI74_004075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015995291.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
     gene            7720..8094
                     /locus_tag="NBI74_004076"
                     /pseudo
     CDS             7720..8094
                     /locus_tag="NBI74_004076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000885598.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
     gene            complement(8149..>8331)
                     /locus_tag="NBI74_004077"
     CDS             complement(8149..>8331)
                     /locus_tag="NBI74_004077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HCD1934328.1"
                     /translation="DGVVHNSFGQKLMRIYNQKGIFSNTKDSEEGLTHILSEHFENVK
                     TKVKGTVVMFSASGKK"
BASE COUNT         1993 a         2201 c         2162 g         1975 t
ORIGIN      
        1 atcggcacac catgcctgat gcgatgctgg tgcgtcttat caggtctaca ggcggtgttg
       61 gaacggtagg ccggataagg cgttcacgcc gcatccggca atcaaatgag caccaggtta
      121 actgaactta ctctgcgccc acatcacgcc gctggcatac tccggtggca gcagcgggat
      181 taaggcttcc agcgtcgcgg tcagatgcga tgtgtcgctg tcggtcaaat ttaaatgccc
      241 cactttacgg cccggacgga cttctttgtc gtaccagtgc agatgcacaa gcggcagttt
      301 cagccagtca taattcagat cgctaccaat cagattgatc atcaccgacg gattattcac
      361 tactggttgc ggtaacggca gatcggtaat tgcccgcaga tgtagctcaa actggctgat
      421 gctggcaccg ttttgcgtcc agtgcccact gttatgcaca cgcggagcca gttcgttgat
      481 cagcagacct tgcggcgtga caaaacactc catcgccatc acgcccacat agcccagctc
      541 ctgcataatc gccgacaaca tctcttcagc ttgcgcctgc agctgtgcgt tggcctgcgg
      601 gaaagcgacg ctggtgcgca aaatgccgtc ctgatgcagg ttatgcgtca gcggataaaa
      661 cacggtgctg ccatcaaaac cgcgcgcgcc aaccagcgac acttcacctg agaagtttat
      721 gccctgctcg acaatacatt cgccgtagca ttcagccggt aactgttcgg tttcagcggc
      781 gcgtaaacgc cattgaccgc gtccgtcata gccaccagtg cggcgcttaa caatcgccag
      841 ctcacctaaa cgatcaaaca ccgcaggcca ctcgctgcgt tcggcaagta actgccacgg
      901 tgcggtcggc aggtggagct tatcgaaaag ctgcttctga gtcagacggt cggcaataat
      961 cgggaacaca tcgcggttca caaaggccgg ctggcgcgcc agctcgcggg ttaatgcagt
     1021 ttccggccag cgttcgatct cagcggtaat cacgctttgt tgaaaaggca ccgccgccgg
     1081 ttcagcgtcc agcccgaccg gccagacagc aatgcctaac ggttcgcctg cctgacgcag
     1141 catacggcct aactgcccgt taccgaggac gcaaacctgt ttcatgccgc acctcgcggg
     1201 tccgggtttt ccagcacttc atcagtttgt gctttgcgcc agtcattcag acgctggtgc
     1261 agttctttat catgagtcgc aagaatttgt gctgccagta acgccgcgtt tgccgcgcca
     1321 gctttaccaa tcgccagcgt acccaccgga atgccgcgcg gcatttgtac gatggagtag
     1381 aggctatcga caccgctcag tgcggcgctc tgtactggca cgcccagcac cggcaccagc
     1441 gttttggcgg caatcatgcc aggcaggtgc gctgcgccgc ctgcgcccgc aataatcacc
     1501 tgataaccgt tctcttcggc gctttcggcg aagctgaaca gtttatcggg ggtgcggtga
     1561 gcagaaacca cttcaacgtg gtgcgggaca ttcaggattt cgaagatttc ggcggcgaac
     1621 tgcatggtag cccagtcgct tttggacccc atcacgatgg cgacacgcgc cggattattg
     1681 cgggaagaca tgcgtcttaa aactcctgtg gtgcacaact ctcggcttta gagggcacag
     1741 agaatagcac ggaaagcgtg caaggaaaac ggttgcgtag gcggaattaa ccgtcagttg
     1801 ttgagttttt agtctgctct gcttgttgat tatgcgctct ggtggcactg gcaaaaaaag
     1861 cccatatcag cacaatgctc cacggtaaaa agcatatacc gacaatcaat atatatcttt
     1921 ctacaaaacg ttggttatgg gggcgggtga gtactaaata aatgagccag aaatagacta
     1981 tccaggaaat gaccgccgca gctaccagtg acaatgcgcc aaaatagaag ccgacaagac
     2041 cgaaccagct aatacacagt ggcgctcctg ccgtaagggc gatgccagaa atcaaaaatc
     2101 cgttgcagcg ttgcataata atattccctg ttgttatttc actcagagat tattaccttg
     2161 ctgattttaa aaaagatata caggttgcgt tagttcggtg ctttttcaga atctgtccat
     2221 tcgtggtcag aaaggaaaat ggattagctc aacgtcatct gccgtgactt tcaccatcga
     2281 accttctgta tgccaggcac ccagtaccac gcgaaaagcc gtttgctgat tggcgataag
     2341 ttcatgcacc gccgggcgat gggtatgccc gtggatcagc cattgcactt gatgtttttc
     2401 catcgcactg accaccgcgt tttggttaac gtccatgatc gccagcgatt tactgctgtt
     2461 ggcttcttta ctgttcgctc gcattcgcgc ggcaatgcgt ttgcgcacaa acaacggcag
     2521 ggcgaggaat agcgtttgca gccagggttt gtggactttg gcgcgaaaag cctgataacc
     2581 cgcgtcatcg gtgcacagcg tgtcgccatg cataatcaac acccgacgac cataaagttc
     2641 gagcaccttt tcttccggca ataacgtcat gccgctttca cgggcaaagc gtttgccgag
     2701 cagaaaatca cggttgccat gaatgaaata acagggaacg ccggaatcgg ataccgcttt
     2761 gatcgctgcg gccatctggt gatgaagtgg gttgggatcg tcgtcgccga tccaggcttc
     2821 aaacagatcg ccaagaatat acagcgcatc ggccttgcgg gcctcccccg ctaaaaaacg
     2881 cagaaaaccg gcggtgatcg ccggttcttc cacgcagaga tgaagatctg caataaagag
     2941 tgtcgccacg attactcgct aacggtcacg ctttcaatga taacgtcttc ttttggcacg
     3001 tcctggtgca taccgctacg accggttgcc acacctttga ttttgtctac cacgtccatg
     3061 ccttcaacca cttcagcaaa tacgcagtag ccccaacctt gcaggctttc gccagagaag
     3121 ttcaggaagt cgttatcaac cacgttgatg aagaactggg cagttgcaga gtgcggtgcc
     3181 tgagtacggg ccattgccag cgtaccacgg gtatttttca gaccgttgtt ggcttcgttt
     3241 ttgatcgggt ctttggtggc tttttgtttc atgcccggtt caaaaccgcc gccctgaatc
     3301 ataaagccgt tgataacacg gtggaaaatg gtgttgttgt aaaaaccttc gcggcagtag
     3361 tccaggaagt ttttaactgt ttcaggtgct ttatcgtcaa aagttttgat gacaatatcg
     3421 ccgtgattgg tgtggaaagt aaccattttt gcatcctgtt ccgtttgatt ggtgcttcaa
     3481 cccggttcgg gtcatatata gggtggtgtt atagcataac cgcacgatcg gatcatctcg
     3541 caatgtatgc tgattcgcgc gggaaatatg ggtattatac gcaactcaat tacccacaca
     3601 tgtctaaacg gaatcttcga tgctaaaaat cttcaatact ctgacacgcc aaaaagagga
     3661 atttaagcct attcacgccg gggaagtcgg catgtacgtg tgtggaatca ccgtttacga
     3721 tctctgtcat atcggtcacg ggcgtacctt tgttgccttt gacgtggttg cgcgctatct
     3781 gcgtttcctc ggctataagc tgaagtatgt gcgcaacatt actgatatcg acgacaaaat
     3841 catcaaacgc gccaatgaaa atggcgaaag ctttgtggcg ctggtggatc gcatgatcgc
     3901 cgaaatgcac aaagattttg atgctttgaa cattctgcgc ccggatatgg agccgcgcgc
     3961 gacgcaccat atcgccgaaa ttattgaact cactgaacaa ctgatcgcta aaggtcacgc
     4021 ttatgtggct gacaacggcg acgtgatgtt cgacgtcccg accgatccga catatggcgt
     4081 gctgtcgcgt caggatctcg atcagctgca ggcaggcgcg cgcgttgacg tggtcgacga
     4141 caaacgcaac ccgatggact tcgttctgtg gaaaatgtcg aaagagggcg aacctagctg
     4201 gccgtctccg tggggcgcgg gccgtcctgg ctggcacatt gaatgttcgg caatgaactg
     4261 caagcagctg ggtaaccact ttgatatcca cggcggcggt tctgacctga tgttcccgca
     4321 ccacgagaac gaaattgccc agtccacctg tgcgcacgat ggccagtatg tgaattactg
     4381 gatgcactcc ggtatggtga tggttgaccg cgagaagatg tctaaatcgc tgggtaactt
     4441 cttcaccgtg cgtgatgtgc tgaaatacta cgatgcggaa actgtgcgtt acttcctgat
     4501 gtcgggtcac tatcgcagtc agttgaacta cagcgaagag aacctgaaac aggcgcgtgc
     4561 ggcgctggaa cgtctctaca ctgcgctgcg cggcacagac aaaaccgttg cgcctgccgg
     4621 tggcgaagcg tttgaagcgc gctttatcga ggcgatgaac gacgatttca acactccgga
     4681 agcctattcc gtactgtttg atatggcgcg tgaagtgaac cgtctgaaag cagaagatat
     4741 ggcagcggcg aatgcaatgg cgtctcacct gcgtaaactt tctgccgtat tgggcctgct
     4801 ggagcaagaa ccggaagcgt tcctgcaaag cggtgcgcag gcagacgaca gcgaagtggc
     4861 tgagattgaa gcgttaattc aacagcgtct ggatgcccgt aaagcgaaag actgggcggc
     4921 ggcagatgcg gcgcgtgacc gtcttaacga gatggggatc gtgctggaag atggcccgca
     4981 agggaccacc tggcgtcgta agtaattgcg ctattgccgg atgcgagttt tcgcatccgg
     5041 ttatcgtctg cgccaccaca acattcccat cagtaacatc cccggcaacc acacccacat
     5101 caattcagaa ataatcacct gatgcccgta cggcgtggtg taacgagaca atgcaaacgg
     5161 cgcgacttta atcacctgcc agggagcgaa aaagcgttca tctgaccacg gccacagcca
     5221 gccaacccat ttaccgccag tggttaccga atccagcaag ctgtgcgata gcaacgagac
     5281 ggttaaaaac agccagcagc gaatcagccc agccctgaac catcgacgtc caatcagcac
     5341 acataacagc gggacaacaa acgcaaacac cagcgaatgg gtaaacccgc gatgaccaaa
     5401 aacatttccg taagcaacgc caaatttaaa cgacaataca tcggcatcgg gcagcatcgc
     5461 caggatgatt ccggcaaata acagacgcgg agggatggct ttcgaaccca accctaaacc
     5521 aatgcatagg ggaacggcgg cgtgcgtaat aacggttggc atgatggtcg cttcggcaaa
     5581 atgtcgatgc tatcagcatg gatgaacggg gcgtagaggg caaaagtctg aaaagagaac
     5641 cggcctgttg atacaggccg ggaaagggat caggcaacaa cctgtacgct gtgacctgca
     5701 aaactcactg tctgaccggc gacgattttg cagcgtttgc gcgtttcaac cgcaccgtcg
     5761 actttcacca ggccttcggc tatcgcgatt ttcgcctgcg cgccgctttc gctccagcct
     5821 tccagtttca gcaagtcgca cagctcaacg tgcggatgtt tacctaaaga aaatgtcgcc
     5881 atgttactca ccctgtggat catgatattc aacgcacgcc tgtagcgtgt tttcaatcag
     5941 cgtggcaacc gtcatcgggc caacgccgcc cggaacaggc gtaatgtatg aggcgcgttt
     6001 agccgcgtct tcaaacacga cgtcgcccac aactttgcca ttttccagac ggttgatgcc
     6061 gacatcaatc acaattgcgc cttctttgat ccagtcacca ggaataaagc ccggcttgcc
     6121 gacggcaacg atcaacaaat cggcattttc tacgtggtga cgcagatttt tagtgaaacg
     6181 gtgagtcacg gtggtggtgc aacctgccag cagcagttcc atgctcattg ggcgaccgac
     6241 gatattcgat gcgccaatca ccacggcgtt aaggccgaag gtatcaatgt tgtaacgctc
     6301 aagcagcgtg acgataccgc gcggggtgca gggacgcaga cgtggcgcgc gctggcacag
     6361 acgaccgacg ttgtaaggat ggaaaccgtc cacgtctttg tccggatgaa tacgttccag
     6421 cactttgacg ttatcaatac ccgctggtaa cggcagttga accagaatgc catcaatggt
     6481 gttgtcggca ttcagcgcat cgataagctc cagcagctcc gcttcgctgg tggtttccgg
     6541 gaggtcataa gagcgggaga cgaacccgac ttcttcacaa gccttgcgtt tgcttgcgac
     6601 ataaatttgc gatgcagggt tgctacccac cagcacaacg gccagtcctg gcgcccgcag
     6661 tccggctgca atacgcgcct gaactttttg agcaacttca gagcgcacct gctgcgcaat
     6721 cgttttaccg tcaataatct ttgctgccat cagagagagg attccatctg ttacgtaggt
     6781 cgaaggggat gtgtctattt tgtcagaagc gtggcgcgct gtcagttttc gttttctgaa
     6841 aagtaaaacc ttctgtacct ggctgattca cacctatcat gataaccgtc gttggtttgc
     6901 cgccatagtg agctacatca gatgcggtcg tacacttgtt cagcaactgg ttcaaaagcc
     6961 attgactcag caagggttga ccgtataatt cacgcgatta cagcacaccg tggtatcaat
     7021 gcgcccttag ctcagttgga tagagcaacg accttctaag tcgtgggccg caggttcgaa
     7081 tcctgcaggg cgcgccatta aaattcaatc agttacgcct tctttatttc ttctataatt
     7141 ccagagtggg acattatcac caaaaatgtc gtctattttc ctcgcgtgct cagtcaaatg
     7201 attaggcgca aggtgagcat atctacgaac catttctctg gactcccatc cgcccatttc
     7261 ctgaagcact gataatggga cgcctgacgg aatcaacctg cttgcccagg tatgtctgag
     7321 gtcatggaaa cggaaatttt caactcctgc acgacgacaa gctgatagcc atgctgtctt
     7381 gctgtcaatg tgcatcttcc tgaccgcagg cgttgatgtt ccatctgctc gcttagccgc
     7441 cttgtatgta cacacacatt tgtgatgctt gcctgtttga tcacgcaaca ctttagaggc
     7501 ggtatcgttc agcgccacac caatggcgcg gtttgatttg ctatcttctg gattcaccct
     7561 ggcaacttgt cgctgcatgt caatctgttg ccactccaga tttatgatgt tcgactttct
     7621 cagaacagtt accagcgcaa acttgacgac agatttcagt agttcggggc tctcatcaat
     7681 caggcgtttt gcctgattct tttgtaagtc tcttgtgcca ttccacctca tacaggtttg
     7741 cctgcaaaca gtaccgatat tgcaccgcca gatattccgg ctggcttcgt ggttgttttc
     7801 aacagtgatg aggcatcgtg gcatctcgtt gaagaccatc ggggtaaaac ggtttatgac
     7861 gtggcatcag gggacgcgtt atttatttct gaactcggtc cgttaccgga aaatgtcacc
     7921 tggttatccc cggcagggga gtttcagaag tggaacggca catcatggat aaaggatacg
     7981 gaggaagaaa cctcgttgct ggaagcctgg aagatgtatc gggtgttgct gaaccgagtt
     8041 gatacatcaa ctgcccctga tattgagtgg cctgtgaacc ctgtcagaga gtaatcattg
     8101 ggattatgcc gcagcacgtc gtgcgcaaga acgtgctgcg tctggatgct attttttccc
     8161 tgaagcggaa aacattacta cagtaccttt aaccttggtt ttaacattct cgaaatgctc
     8221 tgagagtata tgtgttaagc cttcttcgga atcttttgtg tttgaaaaga tgcctttctg
     8281 attgtaaatg cgcatcagtt tttgaccgaa gctattgtgc acaacgccgt c
//
LOCUS       DAHHTG010000169         8302 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.172, whole genome shotgun sequence.
ACCESSION   DAHHTG010000169 DAHHTG010000000
VERSION     DAHHTG010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8302)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8302)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8302
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.172"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            157..492
                     /locus_tag="NBI74_004078"
     CDS             157..492
                     /locus_tag="NBI74_004078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4102 domain-containing protein"
                     /protein_id="HCD1934329.1"
                     /translation="MAILITQDRQVANLTPPEGKSRITVSVKSKVGSGLSVESRSGSQ
                     TKSWIYRPYLNGKQIKITLGSYPAMTLSQAREAHADALALVKQGIDPRYARKTEKQQN
                     EQMPIFSQL"
     gene            700..1476
                     /locus_tag="NBI74_004079"
     CDS             700..1476
                     /locus_tag="NBI74_004079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000171155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCD1934330.1"
                     /translation="MTLDHAALQGLIDHNPARLLKPAMFGASMGKPRERWLPQDELQM
                     LWKALDEAGFGGGSVATGGRGIASSVILSQAIANALRLIILTGVRRSEAVNMRWEQIN
                     GNRWTIPETKNGKSHIVTLHPLALSLLKTQRIISEGGWVFESLSKPSFPVTGDAITRA
                     LERLKTKYMAEVAPFSPHDLRRSIATGCAEYLDAPERLIELLLNHVPKDRLIRTYQVG
                     QQAEKLRNLFLKWGDFIEHEIIESDNGTPDNVIQISFGKR"
     gene            complement(1641..2561)
                     /locus_tag="NBI74_004080"
     CDS             complement(1641..2561)
                     /locus_tag="NBI74_004080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000365807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HCD1934331.1"
                     /translation="MDLRDSYARQVKLLMAALPHVAKESCFALKGGTAINLFVQDFPR
                     LSVDIDLAYKSFADRDTDLAAINDALKRITASLNGRPGITAIRQENKADEKRIIVNTA
                     DAKIKIEVSPVWRGLLLPPAEMPVCERVEMEYGFTTMSVVSLADLYGGKICAALDRQH
                     PRDLFDVLNMLGRPGLTREIFDGFLCYLAGHPRPIAELLASNWDTDRIATLYAQEFSG
                     MTQQETSLESLLSVTTLLPQALKSHFTDLDREFLLSFKQNSPDWSRYRYPEIQHLPAI
                     RWKQRNLAMLKDKNPAKYVAAVNKLERVLE"
     gene            complement(2548..3330)
                     /locus_tag="NBI74_004081"
     CDS             complement(2548..3330)
                     /locus_tag="NBI74_004081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001424109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HCD1934332.1"
                     /translation="MPNYLNWLMQNTLPGQVLLQSWLTRNGVDRKLSYLYVQNGWLRR
                     IAHGVYCRTGREPDWVDAVYCLQTQWEKPVRVAGLTSLALQGHAHYTEPGKPQVWLAL
                     PAYVKLPGWFAAFEQSATFITLYTSGLTVDVSSFTTELKIGDRQLAGSAPELAAYEIA
                     CGVPSLISFEHADALFQGGNLLSPRKLQALLRASHSIKTNRVMLYLARRNGHPYFQYL
                     DQSGIETGTGKRQIIPGGWLEPDYQITVPRTFKPEGVEDGSA"
     gene            3614..6712
                     /locus_tag="NBI74_004082"
     CDS             3614..6712
                     /locus_tag="NBI74_004082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001461529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HCD1934333.1"
                     /translation="MKFTEDTRVKIPVILHLIRLGYHYLSLKEQRWDKETNIFPDLFT
                     AAIGRINPGLAPDDIGRLLKDLTLLLDNDDLGRAFFEKLSDRSGIKLIDFDNFNNNSF
                     HVVTELTCENGDEEFRPDITLLINGMPLIFIEVKKPNNREGIVAERNRINERFKNPKF
                     KRFANITQFMIFSNNMEYDDSDPEPIQGAFYASSSYSNPVFNYFREEELFNLTNLLKP
                     LSEETEIAVLKDNNLEVIRTNPEFQTNKDHNRPTNRLCTALLSRDRLAFILRYALVYV
                     AEPGGIQKHIMRYPQLFATKAIERKLNAGVKKGIIWHTQGSGKTALAYYNVRFLTDYF
                     QNQQVIPKFYFIVDRLDLLQQAQREFTARGLIVHLIDSREAFTRDIKATQALHNHHGK
                     AEITVVNIQKFRDDPDVVTTKDYDINIQRVYFLDEVHRSYNPKGSFLANLSQSDTNAI
                     KIGLTGTPLLGTDYNSRDLFGDYIHKYYYNASIADGYTLRLIREEIATNYRMILQQAL
                     KEVEVRMGDVDRKYIYAHPSFVKPMLNYIVTDFAKSRGALNDDTIGGMVICDSSEQAK
                     QMFEIFNTDYAESPTAADEVSEEMPVLQAAEPAAVYHLHAKETRKIKRAALILHDIGS
                     KQERKEWVEQFKAGKIDLLFVYNMLLTGFDAKRLKKLYLGRVIRKHNLLQALTRVNRT
                     YNHFRYGYVVDFADIRQEFDATNKAYFEELQAELGDEMEHYSRLFKSAEEIREEIEHI
                     KDVLFSFDTENAEIFTDQINQIQDRETALALKNALADARSLYNLIRLQGNTEFLQALD
                     FNKLNILYREASRRLDLLNLKADLEQGVDLSGLLNRALEEVIFEFHKVGEEELVLADE
                     LKETLRRTREALAANFDQQDPQFVSLKDELERLFKKKKLSEVTQQEMVANIGTLNKIH
                     GRIKELNRQNNLLRQKYRGDVKYARTHKRLRERGGLSEPERKIFEALLSVKLDADEKV
                     LNNSQILNNESYFERHMESCVITHFEDEHHITLTSSAVSDINRLIVAEYLNEFNTGAR
                     TW"
     gene            6706..>8302
                     /locus_tag="NBI74_004083"
     CDS             6706..>8302
                     /locus_tag="NBI74_004083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000229742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent DNA methyltransferase"
                     /protein_id="HCD1934334.1"
                     /translation="MVELEFRDKTKALIDSLKSICANYGLGNDGNEFKIITQAFLYKF
                     LNDKFAFEAKQKDKSIASAESWEDALSAMSEDQLKKLQQRMAPDTARLKPHHFIRYLY
                     NRQNAADFARTFDDTLMDIAATNNDVFAVKTDGGAKVVLFERLSQYIADESKRDDFCR
                     AIINKLADFSFERIFTQKFDFYATIFEYLIKDYNSNSGGKYAEYYTPHAVARIMAEIL
                     VPKAQQGVVRNVSCYDPSAGSGTLLMNVAHAIGEDRCSIFAQDISQKSSSLLRLNLIL
                     NNLVHSIPNVIQGNTILHPFHKDGGALKRFDYIVSNPPFKMDFSDFRDALDSKENQQR
                     FFAGIPKIKAKARDKMEIYQLFLQHIIFSLKPGGKAAVVVPTGFITAQSGIDKGIREH
                     LVQNKMLAGVVSMPSNIFATTSTNVSILFIDASNKEKVVLIDASNLGEKVKDGKNQKT
                     VLTECEEKRICEAFNNKWSEEDFSVVVSYDDIAAKNYSFSAGQYFDVKIEYTDMTPEQ
                     FAAKMKGFTENLNNLFEQSRELEV"
BASE COUNT         2326 a         2269 c         1973 g         1734 t
ORIGIN      
        1 tctatttttc cgcatatcta cacgtctcta taaatctcat aaaaatatta aaaatcatat
       61 aattaaacaa aaaacccatc ttatattgtt ccatcgaatt tcgctctata caatgctaaa
      121 caagtacgcc agcaagtacg cgagatgagg ccaaaaatgg caatattgat aacccaggac
      181 aggcaagtcg caaacctgac tccccctgaa ggaaaaagtc ggataaccgt cagcgtcaaa
      241 tcaaaagtag gttccggcct gtctgtcgaa tcccgttccg ggagtcaaac gaagagctgg
      301 atttaccgcc cttacctcaa cggaaaacag attaaaataa cgttaggctc ctaccctgca
      361 atgacattgt ctcaggcgcg tgaagcacat gcagacgcgc tggcgctggt taaacaaggt
      421 attgatccgc gttatgcgcg aaaaacggaa aaacagcaaa atgaacaaat gccgattttt
      481 tcacaattat gaaaaaactg gctggcattc cgtgaaacca ataaacccat tggggcgcgt
      541 actctggctg attatgaggg aacatatcgc cgccatctgg agaaaggttt aggttcagta
      601 cgcgtctgcg acctgtccag agcctacatt tatgaacatc tcatacggat cagaaagacc
      661 agcgccgaag gtgtccgcaa gggacttatt cttctcaata tgacgcttga ccatgccgct
      721 ttgcagggat tgatcgatca caaccctgct cgcttgctta aacccgcaat gtttggtgcc
      781 tcaatgggaa aaccgcgtga gcgttggttg ccacaagacg agttgcaaat gttatggaag
      841 gcactggatg aagcggggtt cggcggcgga tctgtcgcaa ccggagggcg aggcatcgcg
      901 tccagcgtta ttctttcaca agcgattgct aacgcattac gcctcatcat cctgacgggg
      961 gtgcgacgtt ccgaagcggt gaatatgcgt tgggagcaga ttaatggcaa ccgctggaca
     1021 atacctgaaa ccaaaaacgg taaaagtcat atcgtgacgc tacatcctct ggcgctttct
     1081 ctattgaaga ctcagcgcat tatttcagaa ggcggctggg tattcgagtc gctcagtaag
     1141 ccaagttttc ctgtcaccgg tgacgcaata actcgtgctc ttgaacgcct gaaaacaaaa
     1201 tatatggcag aagtcgctcc tttctcacca catgatttac gccgcagcat tgctacaggc
     1261 tgcgctgaat atctggacgc accagaacgg cttatagaac ttctgttaaa tcatgtgccc
     1321 aaagacaggt taatcagaac ctaccaggtc gggcagcaag cggaaaaatt acggaatctg
     1381 tttctgaaat ggggggattt tattgagcat gagatcattg agtctgataa tggtactcca
     1441 gataatgtta ttcagatcag ctttggaaag agatagtttc gtattttcat gagtagagat
     1501 cgtctgataa ctcagcgatc tcttactcga tggtgctatg tattgaatta aatgaatatg
     1561 tttgagttat tgccacgaag ttattcatat aaccaaagca tcatggccca attcagagtc
     1621 cctgaacagc gctaagaata ttactccagc accctctcta gcttattcac cgccgccaca
     1681 tacttcgccg gatttttatc tttaagcatc gccagattgc gttgcttcca gcgaatagcc
     1741 ggaagatgct gaatttcagg atagcgatac cgcgaccagt cagggctgtt ttgcttaaaa
     1801 ctgagcaaga actcgcgatc cagatccgta aaatgtgact tcaacgcctg cggcagcaaa
     1861 gtcgttactg acagaagcga ttccagcgac gtttcctgct gagtcatccc cgaaaattcc
     1921 tgagcgtata acgtggcaat ccgatctgta tcccagtttg aagccagcaa ctcggcaatg
     1981 ggacggggat ggcctgccag ataacaaaga aaaccgtcaa aaatttcgcg cgtcaggccc
     2041 ggtctcccca gcatattcaa cacatcaaac agatcgcgcg gatgctgacg atcaagcgca
     2101 gcacatattt taccgccata aagatccgcc agcgagacga cactcatggt ggtgaagcca
     2161 tactccattt ccacacgttc gcacacaggc atttctgccg gaggcaacaa cagcccgcgc
     2221 cagaccgggc taacctcgat cttaatcttt gcatcagcgg tattcacaat gatgcgcttt
     2281 tcatccgctt tattttcctg ccgaatcgcg gtgatccccg gtctgccgtt cagcgatgca
     2341 gtaattctct tcaaagcgtc gttgatcgcc gccagatcgg tatcgcgatc cgcaaagctc
     2401 ttatacgcca gatcgatatc caccgataga cgggggaaat cctgcacaaa taaattgatt
     2461 gctgtgccgc ctttcaacgc gaaacaggat tccttcgcca catgcggcag agcggccatc
     2521 aacagtttaa cctgcctggc atagctatca cgcagatcca tcttcgactc cttccggttt
     2581 aaatgtgcgg ggaacagtaa tttgataatc cggctccagc cagccaccag gtatgatttg
     2641 ccgctttccc gtacctgttt ctataccgct ttgatcaaga tactgaaaat aagggtgtcc
     2701 attacgccgg gcaaggtaaa gcatcacccg gttggtttta atcgaatgac tggcgcgaag
     2761 cagtgcctgc aatttgcgtg ggctgagaag attccccccc tgaaacagag catcggcatg
     2821 ttcgaacgag atcaacgaag gaacgccgca ggcgatctca tacgcggcca gttccggtgc
     2881 actgcctgct aactgccggt cgccaatttt cagttccgta gtaaatgacg agacatcaac
     2941 ggtaaggccc gatgtataca gcgtgataaa tgttgctgat tgttcgaagg cagcaaacca
     3001 tcccggcagt ttgacgtaag ccggaagcgc gagccagacc tgcggcttac ccggttcggt
     3061 atagtgcgca tggccctgaa gcgcaaggct ggttagcccc gccacccgaa caggcttttc
     3121 ccactgtgtt tgaaggcagt aaaccgcgtc tacccaatcc ggctcacggc ccgtccggca
     3181 gtacacaccg tgagcgatgc gcctgagcca gccattctga acatagagat aagaaagctt
     3241 acgatctacg ccattgcggg taagccatga ctggagcagc acctgaccgg gtagcgtgtt
     3301 ttgcatcaac cagtttagat agtttggcaa atgtcgctcc tgaatagaca ttagtagaaa
     3361 tatttaaacc agtgtaggtt cagggatacg ccacagcaag aaatagttta atgattcctc
     3421 atttcgtcca cttaaaatcg acattgagca aaaaatacaa acttacctac attcaggcaa
     3481 agtgcgacaa atagactcgg tcggtttaaa gagaacgtgt aacatagatg caaatcgaac
     3541 agacattgtt gccagccgtt ccaaggttgt ggggcgctgc atggcctgag acgtgataaa
     3601 tacagggaac acaatgaaat tcactgaaga tacccgagta aaaatccccg tcatcctcca
     3661 cctgatacgg ctgggctacc actacctgtc ccttaaagaa caacgctggg acaaagaaac
     3721 caatatcttc cctgacctct tcaccgctgc gattggcaga ataaatcccg gacttgcgcc
     3781 agacgacatc ggacgcctgt taaaagacct cacgctgctt ctcgacaacg atgatctggg
     3841 ccgcgccttc ttcgaaaaac tcagtgaccg ttccggtatc aaactgatcg actttgataa
     3901 cttcaacaac aacagtttcc atgtcgtcac cgaactgaca tgcgaaaacg gtgatgaaga
     3961 attccgcccg gacatcacgc tgctgatcaa cggcatgccg ctaatcttta tcgaagtgaa
     4021 aaaacccaac aatcgggaag gcatcgtcgc agaacgcaac cgcatcaacg aacgctttaa
     4081 aaaccccaaa ttcaaacgct tcgccaacat cacccagttc atgatcttct ccaataacat
     4141 ggagtatgac gacagcgacc cggagccgat tcaaggggca ttctacgcca gcagctctta
     4201 cagcaacccg gtattcaact acttccgcga agaagaactc ttcaacctga ccaacttact
     4261 gaaaccgctg tcagaagaaa ccgaaatcgc cgtcctgaaa gacaacaacc tggaagtgat
     4321 ccgcaccaac ccggaatttc agaccaacaa agatcacaac cgcccgacaa accgcctctg
     4381 caccgcgctg ttgagccggg atcggctggc atttatcctg cgctatgcgc tggtgtatgt
     4441 agccgaaccg ggcggcatcc aaaaacacat catgcgctac ccgcagctct tcgccaccaa
     4501 agcgattgag cgcaagctga acgcgggtgt gaaaaaaggc attatctggc acacccaggg
     4561 cagtgggaaa accgcattgg catactacaa cgtgcgcttc ctgacagatt acttccagaa
     4621 tcagcaggtg atcccgaaat tctactttat tgtcgatcgt cttgatctgc tccagcaggc
     4681 gcagcgtgaa ttcaccgccc gtggcttaat cgttcacctt atcgactccc gcgaagcctt
     4741 cactcgcgat atcaaagcaa cgcaggcgct gcacaaccac cacggcaaag cggaaatcac
     4801 cgtcgtcaac atccagaaat tccgcgatga cccggatgtc gtcaccacca aagactacga
     4861 catcaacatc caacgcgtgt acttcctcga tgaagtccac cgcagctaca accccaaagg
     4921 gagcttcctg gcgaatttaa gccagtcgga caccaacgcc atcaaaattg gcctgaccgg
     4981 tacgcctctg ctgggcacag actacaactc ccgcgacctc ttcggcgact acatccacaa
     5041 atactactac aacgcctcca tcgccgacgg ctacacgctg cgcctgatcc gcgaagagat
     5101 cgccaccaac taccgaatga tcctgcaaca ggcgctaaaa gaggtggaag tcagaatggg
     5161 cgatgtggat cgcaaataca tctacgccca tcccagcttt gttaaaccga tgctgaacta
     5221 catcgtcacc gactttgcca aaagccgggg cgcgctgaat gatgacacca tcggcggcat
     5281 ggtgatttgc gacagctctg aacaggccaa gcagatgttt gagatattca acactgacta
     5341 cgccgaatcg cccaccgccg ccgatgaagt ctccgaagaa atgccggtat tacaggccgc
     5401 agaacctgcc gccgtttacc acctccacgc caaagaaacg cggaaaatca aacgcgccgc
     5461 cctgatcttg cacgacatcg gcagcaagca ggagcgcaaa gaatgggtgg agcaattcaa
     5521 agcgggcaag attgacctgc tgttcgttta caacatgttg ctcaccggct tcgacgccaa
     5581 acggctgaaa aaactctacc tcgggcgggt gatcagaaag cacaacctgc tccaggcgct
     5641 gacccgcgtc aaccgtacgt acaaccactt ccgctacggc tacgtggtgg acttcgccga
     5701 catccgccag gaatttgacg ctaccaacaa agcctacttc gaagagctac aggctgaact
     5761 tggcgacgaa atggagcact actcccgcct gttcaaatcg gcggaagaga tccgcgaaga
     5821 aatcgaacac atcaaagacg ttctgtttag cttcgatacc gaaaacgcgg aaatcttcac
     5881 tgaccagata aaccagatcc aggaccggga aaccgcgctg gcgctaaaaa acgcgctggc
     5941 cgacgcccgc agcctgtaca acctcatccg cctccagggc aacactgagt ttctccaggc
     6001 gctggatttc aacaaactca acatccttta tcgcgaagcc agccgccgcc tcgacttact
     6061 caacctaaaa gcggatctgg aacagggcgt tgacctctcc ggcctgctga accgggcgct
     6121 ggaagaggtg atctttgaat tccataaagt gggtgaagaa gagctggtac tggccgacga
     6181 gctgaaagag acgctgcgcc gcacccgcga agcgctggcc gccaacttcg accagcagga
     6241 cccgcagttc gtcagcctga aagacgaact ggagcgtctg tttaagaaaa agaaactcag
     6301 cgaagtcacc cagcaagaaa tggttgcgaa tatcggcaca ctcaataaaa ttcatggccg
     6361 catcaaagag ctaaaccgcc aaaacaacct gctgcgccag aaataccggg gcgacgtgaa
     6421 atacgcgcgt acccacaaac gcctgcgcga gcgcggcggc ctgagcgagc cggagcgtaa
     6481 aatcttcgaa gcactgctta gcgttaagct ggatgcagat gaaaaagtgc tgaacaacag
     6541 ccagatactg aacaatgaaa gctattttga gcggcatatg gagtcatgcg tcatcacgca
     6601 ctttgaggat gagcaccata tcactctgac ctccagtgcg gtaagcgaca tcaaccgcct
     6661 gattgtggcg gaatacctga acgaatttaa taccggagca cgaacgtggt agaactggaa
     6721 tttagagaca aaaccaaagc cctgatcgac agcctgaaaa gcatctgtgc gaactacggc
     6781 ctgggcaacg acggtaacga attcaaaatt atcactcagg cattcctgta caaattcctg
     6841 aacgacaaat tcgcttttga ggccaaacag aaagataaaa gtatcgccag cgccgaaagc
     6901 tgggaagatg cgctcagcgc aatgagcgaa gatcaactga aaaagctgca acagcggatg
     6961 gccccggata ccgcccgcct gaagccgcac cattttattc gttacttgta taaccgacaa
     7021 aacgcagcgg actttgccag aacttttgac gacaccctga tggacatcgc cgccaccaac
     7081 aacgacgtct tcgcggtcaa aaccgacggc ggcgcgaaag tggtgctgtt tgagcgcctc
     7141 agccagtaca tcgccgacga atcaaagcgc gatgatttct gccgcgccat catcaacaag
     7201 ctggccgatt tcagcttcga acgcatcttc acgcagaaat tcgacttcta cgccaccatc
     7261 tttgaatacc tgatcaaaga ctacaacagc aactccggcg gcaaatacgc ggaatactac
     7321 acgccccacg ccgtggcgcg catcatggcg gaaatcctgg tgccgaaagc gcagcagggc
     7381 gtggtgcgca acgtaagctg ttacgacccg tccgccggtt ccggcacgct gctgatgaac
     7441 gtggcgcacg ccattgggga agatcgttgc agcatctttg cccaggatat ctcgcagaaa
     7501 tcctccagcc tgctgcgtct gaacctgatc ctcaacaacc tggtgcattc gatccctaac
     7561 gttatccagg gcaacactat tctgcatccg ttccacaagg acggcggcgc gctgaaacgc
     7621 tttgactaca tcgtctccaa cccgccgttt aagatggatt tcagcgactt tcgtgacgcg
     7681 ctggacagca aggaaaacca gcaacgtttc tttgccggga taccgaaaat caaagccaaa
     7741 gccagggaca aaatggagat ttaccagttg ttcctgcaac acatcatttt ctcgctgaaa
     7801 ccgggtggca aggcggcggt cgtcgtgccg accgggttta tcaccgcgca atcgggcatt
     7861 gataaaggca ttcgcgaaca tctggtgcaa aacaagatgc tggcgggcgt ggtttccatg
     7921 ccgtcgaata tcttcgccac caccagtacc aacgtgtcga tcctgtttat tgacgccagc
     7981 aacaaagaga aagtggtgct gatcgacgcc tcgaatttag gtgaaaaggt caaagacggc
     8041 aaaaaccaga aaacggtgct gacggagtgc gaagaaaaac ggatctgcga agcgtttaac
     8101 aacaagtgga gcgaagagga tttctcggtg gtggtcagct atgacgacat cgccgcgaaa
     8161 aattactcgt tcagcgccgg acagtatttt gatgtgaaaa tcgaatacac cgacatgacg
     8221 ccggaacagt ttgccgcgaa gatgaaagga tttacggaga atctgaataa tctctttgaa
     8281 cagtcgcgcg agttagaagt gc
//
LOCUS       DAHHTG010000170         8268 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.173, whole genome shotgun sequence.
ACCESSION   DAHHTG010000170 DAHHTG010000000
VERSION     DAHHTG010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8268)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8268)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8268
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.173"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            complement(<1..338)
                     /locus_tag="NBI74_004084"
     CDS             complement(<1..338)
                     /locus_tag="NBI74_004084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001332039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Alw26I/Eco31I/Esp3I family type II restriction
                     adenine-specific DNA-methyltransferase"
                     /protein_id="HCD1934335.1"
                     /translation="MEEIFYMKHIHLINSLSLDETTKFTKKATGKYYTDPKIALLMIE
                     KLLPLINSCDKKSYNVADPFSGDGRLITLLIKQWMINGFPDVEWNVYLFDIENTGLTY
                     AKNALSELKLA"
     gene            complement(1111..1287)
                     /locus_tag="NBI74_004085"
                     /pseudo
     CDS             complement(1111..1287)
                     /locus_tag="NBI74_004085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611530.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            complement(1651..1809)
                     /locus_tag="NBI74_004086"
     CDS             complement(1651..1809)
                     /locus_tag="NBI74_004086"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1934336.1"
                     /translation="MMMSDTTLSRPEVVSGHTDVIYSTSVCHILAVRKSTLLPIDTII
                     RHQVSDIS"
     gene            complement(1909..2085)
                     /locus_tag="NBI74_004087"
     CDS             complement(1909..2085)
                     /locus_tag="NBI74_004087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF957 domain-containing protein"
                     /protein_id="HCD1934337.1"
                     /translation="MKSLTTETALDILITWLQDNIDCESGIIFDNDEDKTDSAALLPC
                     IAQAREGIRTLRQQ"
     gene            complement(2102..2590)
                     /locus_tag="NBI74_004088"
     CDS             complement(2102..2590)
                     /locus_tag="NBI74_004088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1934338.1"
                     /translation="MKLALTLEADSVNVQALNMGRIVVDIDGVNLAELINMVCDNGYS
                     LRVVDESDRTSADCTPPFVALTGIRCSTAHITETDNAWLYSLSHQTSDFGESEWIHFT
                     GTGYLLRTDTWSYPVLRLKRLGLSKTFRRLVVTLIRCYGVSLIHLDASAGYLPGLPTF
                     DW"
     gene            complement(2587..2964)
                     /locus_tag="NBI74_004089"
     CDS             complement(2587..2964)
                     /locus_tag="NBI74_004089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin"
                     /protein_id="HCD1934339.1"
                     /translation="MKTLPDTHVRKVSRCPSPVTIWQTLLTRLLDQHYGLTLNDTPFA
                     DERVIEQHIEAGISLCDAVNFLVEKYALVRTDLPGFSACTHSQLINSIDILRARRATG
                     LMIRDNYRTVNNITLGKHPEAKR"
     gene            complement(3053..3421)
                     /locus_tag="NBI74_004090"
     CDS             complement(3053..3421)
                     /locus_tag="NBI74_004090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system YeeU family
                     antitoxin"
                     /protein_id="HCD1934340.1"
                     /translation="MSDKLSGITHPDDNHDRPWWGLPCTVRPCFGARLVQEGNRLHYL
                     ADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSGELNPRHQHTVTLYAKGLTCEAD
                     TLGSCGYVYLAFYPTSETKK"
     gene            complement(3584..3805)
                     /locus_tag="NBI74_004091"
     CDS             complement(3584..3805)
                     /locus_tag="NBI74_004091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 domain-containing protein"
                     /protein_id="HCD1934341.1"
                     /translation="MRIITRGEAMRIHQQHPASRLFPFCTGKYRWHGSAEAYTGREVQ
                     DIPGVLAVFAERRKDSFGPYVRLMSVTLN"
     gene            complement(3868..4344)
                     /locus_tag="NBI74_004092"
     CDS             complement(3868..4344)
                     /locus_tag="NBI74_004092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RadC family protein"
                     /protein_id="HCD1934342.1"
                     /translation="MQQLSFLPGEMTPGERSLIQRALKTLDRHLHEPGVAFTSTRAAR
                     EWLLLNMAGLEREEFRVLYLNNQNQLIAGETLFTGTINRTEVHPREVIKRALHHNAAA
                     VVLAHNHPSGEVTPSKADRLITERLVQALGLVDIRVPDHLIVGGSQVFSFAEHGLL"
     gene            complement(4360..4824)
                     /locus_tag="NBI74_004093"
     CDS             complement(4360..4824)
                     /locus_tag="NBI74_004093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708769.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HCD1934343.1"
                     /translation="MKTLSQNTTSSACAPETDLQQLVATLVPDEQRISFWPQHFGLIP
                     QWVTLEPRVFGWMDRLCEDYCGGIWNLYTLNNGGAFMAPEPDDETWVLFNVMNGNRAE
                     MSPEAAGIAACLMAYSHHACRTENYAMTVHYYRLRDYALQHPECSAIMRIID"
     gene            complement(5166..5984)
                     /locus_tag="NBI74_004094"
     CDS             complement(5166..5984)
                     /locus_tag="NBI74_004094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF945 domain-containing protein"
                     /protein_id="HCD1934344.1"
                     /translation="MRLASRFGYTNQIRRDRPLTHEELMHYVPGIFGEDRHTSRSERY
                     TYIPTITVLESLQREGFQPFFACQTRVRDPGRRGYTKHMLRLRRAGEINGEHVPEIIL
                     LNSHDGTSSYQMLPGYFRFVCQNGCVCGQSPGEVRVPHRGNVVDRVIEGAYEVVGVFD
                     RIEEKRDAMQSLVLPPPTRQALAQAALTYRYGDEHQPVTTADILTPRRREDYGKDLWS
                     AYQTIQENMLKGGISGRSAKGKRIHTRAIHNIDTDIKLNRALWVMAETLLESLR"
     gene            complement(6139..6297)
                     /locus_tag="NBI74_004095"
     CDS             complement(6139..6297)
                     /locus_tag="NBI74_004095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001550547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 family protein"
                     /protein_id="HCD1934345.1"
                     /translation="MPGCTSRLLPEGPFSREQAVAVKTAYRNVFTEDDQGTYSRLVIR
                     NAEGQLRW"
     gene            complement(6368..>8268)
                     /gene="ag43"
                     /locus_tag="NBI74_004096"
     CDS             complement(6368..>8268)
                     /gene="ag43"
                     /locus_tag="NBI74_004096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="autotransporter adhesin Ag43"
                     /protein_id="HCD1934346.1"
                     /translation="STAATVLGSNRLGNFTVENGKADGVVLESGGRLDVLEGHSAQKT
                     RVDDGGTLAVSAGGKATGVTMTSGGALIADSGATVEGTNASGKFSIDGISGQASGLLL
                     ENGGSFTVNAGGQASNTTVGHRGTLMLAAGGSLSGRTQLSKGASMVLNGDVVSTGDIV
                     NAGEIYFDNQTTPDAVLSRAVAKGNAPVTFHKLTTSNLTGQGGTINMRVRLDGSNASD
                     QLVINGGQATGKTWLAFTNVGNSNLGVATTGQGIRVVDAQNGATTEEGAFALSRPLQA
                     GAFNYTLNRDSDEDWYLRSENAYRAEVPLYTSMLTQAMDYDRILAGSRSHQTGVNGEN
                     NSVRLSIQGGHLGHDNNGGIARGATPESSGSYGFVRLEGDLLRTEVAGMSLTTGVYGA
                     AGHSSVDVKDDDGSRAGTVRDDAGSLGGYLNLTHTSSGLWADIVAQGTRHSMKASSDN
                     NDFRARGWGWLGSLETGLPFSITDNLMLEPQLQYTWQGLSLDDGQDNAGYVKFGHGSA
                     QHVRAGFRLGSHNDMSFGEGTSSRDTLRDSAKHRVRELPVNWWVQPSVIRTFSSRGDM
                     SMGTAAAGSNMTFSPSRNGTSLDLQAGLETRVRENITLGVQGGYAHSVSGSSAEGYNG
                     QATLNVTF"
BASE COUNT         1963 a         2159 c         2129 g         2017 t
ORIGIN      
        1 gctaacttaa gttcagacaa cgcattttta gcataagtga ggcctgtatt ttctatatcg
       61 aacagataaa cattccactc tacatctgga aaaccattta tcatccattg tttaataagc
      121 aatgtaataa gtcgaccatc ccctgaaaat ggatctgcga cattataact ttttttatca
      181 caggaattta tcaatggtaa taacttctct atcatcaaaa gagctatttt tggatctgta
      241 tagtatttac ctgtcgcttt tttagtgaat tttgttgtct cgtccaagga cagtgaatta
      301 attaaatgga tatgtttcat gtaaaatatc tcttccatta ataatatgga ttatatcatt
      361 taacttgtga acactcaaca ataactgtac ataaaaacag tcattaaaga tttcccctag
      421 agtgagcatc gctataaaaa tttaaattaa ttttatcctg ctccgaaaga aacagttatc
      481 atttattcca taaagaataa cttgctttat aatcacttta aatatcgata tcaattttaa
      541 tactatggtt acttaccata tcaataaata gcttaacccg ttatctcgat accccttaag
      601 aaccccttcc gcaactgcaa cataatctac gctcatccac atgcctggtg cgtattaccg
      661 tgaaggagat cggtgagtta ttgagtgatc tcctttcatg aaaatacgca cctggcttta
      721 actgttcctg atataagttg acagttttct gtccgtaaaa cggttgatga acttcatcgg
      781 gcgttttgta tttcaatgcc aaatgtgacc gctcatggtt ataaattgcc acgaattctt
      841 ttaccattta ccggccctac gtcagctctg caggacgcga gagtaaaaac tcattttcag
      901 gattccgttt atccactcag cctgcgcatt ctggtggcag ttataaccat tagtcattga
      961 acaggttatt gataaataat taaaaaactc agttttctgg ctcgcctgct caagctgttc
     1021 ttcaagttcc ctgattcttt gctcgggtgt cagcgggaca gtcgccagag ccacaggcag
     1081 tctcctcttt acctggtcag gaagtccggg gctccagtca agccggccat atttacgcaa
     1141 ccagacaaga acggtggagc gcccctgaat gccataactg cagccggcct gtttataagt
     1201 catctcgcct ttttcaacct tctcaacaac ggctatttta aaggatagag gataatcacg
     1261 ttgagtgcgt ttgaacccag acatcatcac gttctccggg tttaagtcag gaacgtgtca
     1321 tagctattca ggacgggtca ccgacacaaa aaaagtcgcc cttgagcgac tcgatttgca
     1381 tacggtatgg tgcgaaggcc ggactcaaac atgcgattta acctcatgat ttaaaattgt
     1441 taataataaa caacaccatc ctataccaac acagatacca acacgaaaaa tcactggttt
     1501 tcggcatcga accaggacaa tcaggtcctg attgagatac cgagctctgt tactcgtcat
     1561 cttcctcgac aggctccatt gcatcaagtg gtactcgcac catcgcgata gaaccaatcc
     1621 ggataaaagc atcttcccca tctatatcgg ttaactgata tcgcttactt gatgccggat
     1681 aattgtgtcg attggcaaca gtgtactctt tcgtacagcc agaatgtggc agactgaggt
     1741 ggaatagata acgtccgtat gcccgctgac cacctccggg cgggagagtg tggtatctga
     1801 catcatcatt tttcctttct gtttataaat gaaaatgcca gccgtgttca ggctgacgtc
     1861 agggaagtga aatcgggtga gtgatcttca ctggttctgg tacaaaagtt actgttggcg
     1921 cagggtacgg ataccctccc tggcctgtgc gatacagggc aacagtgctg ccgaatctgt
     1981 tttatcctca tcgttgtcga agataattcc cgattcgcag tcgatattgt cctgcagcca
     2041 cgtaatcaga atatccagcg ctgtttccgt ggttaatgat ttcatgttgt gaatttccgg
     2101 attaccagtc gaaagtgggt aaacccggca gatatccggc actggcatcc agatgaatga
     2161 gactgacacc ataacaccgg atgagtgtga cgaccagacg acggaacgtt tttgacagcc
     2221 ccaggcgctt aagccgcaga accgggtatg accacgtatc ggtacgtaac agataaccgg
     2281 taccggtaaa atgaatccat tctgattcac cgaagtcact ggtctggtgt gacagcgagt
     2341 acagccaggc gttgtccgtt tccgtgatat gtgcggtact gcagcgtatg ccggtaaggg
     2401 caacaaatgg tggcgtgcag tctgctgagg tccggtcaga ttcatcaaca acacgaaggg
     2461 agtagccgtt atcacagacc atgttaatca gttcagcgag attaacacca tcaatgtcaa
     2521 cgacaatgcg ccccatgttc agcgcctgca cgttaacgct gtcggcttcc agcgtcaggg
     2581 ccagtttcat cgtttcgcct ccggatgctt ccccagggta atgttattta ccgttctgta
     2641 attgtcgcgg atcatcaggc ctgttgcccg gcgagcccgg aggatatcga tgctgtttat
     2701 taactgagag tgggtacagg cgctgaatcc cggcaggtcg gtacgtacca gtgcgtattt
     2761 ttccacgaga aagttcactg catcacacag tgaaatgcct gcctcaatat gctgctcaat
     2821 cacacgttca tcagcgaacg gtgtgtcatt cagcgtaagg ccgtagtgct ggtccagcag
     2881 tcgggtgagc agtgtctgcc agatggtgac gggagacggg cagcgcgata ccttccgtac
     2941 gtgtgtatca ggtaatgttt tcatactgaa gattttcctg atatgcagag ataaaaatgg
     3001 aaaagtggcg tggtgaaaac accagaccgg agcagaaggt tattctggag agttactttt
     3061 tcgtttcgga tgttggataa aaagccagat aaacgtaacc acaactgccg agggtgtcgg
     3121 cttcgcaggt cagtcctttc gcatacagcg tgacggtatg ctgatggcgg ggattcagtt
     3181 caccgctggt gagcattaat tccagttgtt tcatcagcag cggaaaggcc tggtccagat
     3241 ggtatgcatc tgcgtcgctg aagcggcctc tgataccggc gcggtcggca aggtaatgca
     3301 ggcggttacc ctcttgcacc agacgagcgc caaaacaggg cctcactgtg cagggaagcc
     3361 cccaccaggg gcggtcgtga ttgtcatcgg gatgagttat cccggagagt ttgtctgaca
     3421 cgataaaatc ctctctgaaa agtggttaag aatggtttaa cgatgacagg tcagccggcg
     3481 cgctgaccgg tttcggtgaa gatgtatgca ttgatgagaa tggcctcatc cacagcggcg
     3541 tctgaggtca tctggtcgta ctcgtttccc gggtgacgga acatcagttc agggtgacac
     3601 tcatcagccg gacatacggg ccaaaactgt ccttacggcg ttctgcaaac acggcaagaa
     3661 cgccgggaat atcctgcact tcacgaccag tatacgcttc agcactgccg tgccagcgat
     3721 acttaccggt acagaacgga aaaagacggg atgcaggatg ctgttggtga atacgcatgg
     3781 cttcaccacg ggtgatgatt ctcatgatgt gatacctctg aagacagaag ataaaagtga
     3841 aaacaggtgt gatgtggtga caacgggtta aagcagaccg tgttccgcaa aggagaaaac
     3901 ctgactgcca ccgactatca gatggtccgg cacccggata tccaccaggc ccagtgcctg
     3961 taccagacgt tccgtgataa ggcggtctgc cttactgggg gtgacttcac cggacgggtg
     4021 attgtgtgcc agcaccacgg cagcggcatt gtggtgcagg gcgcgtttaa tcacttcccg
     4081 gggatggact tccgtgcggt tgatggtgcc ggtgaagagg gtttcaccgg caatcagctg
     4141 attctggttg ttcagataca gcacccggaa ctcttcacgc tccagtcccg ccatgttcag
     4201 aagcagccat tcccgtgccg cacgggtgga ggtgaaggcc acgccgggtt catgaagatg
     4261 gcggtccaga gttttcaggg cccgctgaat gaggctgcgc tcgccgggcg tcatctctcc
     4321 gggcagaaag gaaagctgct gcattgtccc tgcctccatt cagtcgatga tgcgcataat
     4381 ggcgctgcat tccggatgct gcagggcata atcccgcagc cggtaataat gaaccgtcat
     4441 ggcataattc tctgtacgac aggcgtgatg gctgtacgcc atcagacagg cggcaatgcc
     4501 ggcggcttcc gggctcattt cagcgcggtt accgttcatg acgttaaaca gcacccatgt
     4561 ttcgtcatcc ggttcgggtg ccataaatgc gccaccgttg ttcagggtgt acagattcca
     4621 gataccaccg cagtagtctt cacacagacg gtccatccag ccgaagacac ggggctccag
     4681 ggtcacccac tgtggaatga ggccaaaatg ctgcggccag aagctgatgc gctgttcatc
     4741 agggacgagg gtggcaacca gttgttgcag gtcagtctcc ggtgcacagg ccgaagaggt
     4801 ggtgttctga gacaatgttt tcatgtatga aatccttgta atgtgaataa atgagaggga
     4861 gtaaaggtcg ggagctggca acatcacgtt catcactgac cgtgatgact cggtaatatc
     4921 agggatgaat gatattcggc tgaaattccg gacagccgta cgcgtaaccg ttccggtcac
     4981 agacggatgg ccagatgcca ttgcctgtat actcagtctg cacagactga tttctccggg
     5041 acggtaaagc agacgggtac accaggcgca caggtcatca tcagaaatgg aacgacaccc
     5101 cggcagcgcc tccggggcgt cgggtgattc tggtgcctga atcacgcctt cagggacatg
     5161 ctgcatcagc gcaggctctc cagcagcgtt tctgccatta cccacaatgc gcggttgagc
     5221 ttaatatcgg tgtcgatgtt gtgaatggca cgggtgtgga tacgttttcc ttttgcactg
     5281 cggccggaaa ttccgccttt cagcatattc tcctggatgg tctgatatgc gctccacagg
     5341 tctttaccgt aatcctcccg gcgtcgtggt gtcagaatgt cagcggtggt gactggctga
     5401 tgttcgtcac cataacggta agtcagtgcc gcctgtgcca gcgcctggcg tgtcggtggc
     5461 ggcaggacca gcgactgcat ggcatcacgc ttttcctcaa tccggtcaaa cacgcccacc
     5521 acctcgtaag ccccttcaat gactctgtcc accacatttc cccgatgcgg aacacgcact
     5581 tcccccggag actgaccaca gacgcatccg ttctgacaga cgaacctgaa gtaacccggc
     5641 agcatctggt agctggaggt accgtcatgg gagttgagca gaatgatttc agggacatgt
     5701 tctccgttta tctctccggc ccgccgcaga cgcagcatgt gttttgtgta tccccggcgg
     5761 cccgggtcgc gcacacgggt ctggcaggcg aagaacggct gaaagccttc ccgctgcaga
     5821 ctttccagca cggtgatggt ggggatatac gtatagcgtt cactgcggga ggtatgccgg
     5881 tcttccccaa aaataccggg tacatagtgc atcagttctt cgtgtgtcag cggacggtca
     5941 cggcgtatct ggttcgtata accaaaacga ctggctagtc gcataatttg ctccttatcg
     6001 gtggttaaga tttactggtg taataaatga aaaagccacg tctcccggag aagacgcggc
     6061 ctgacagatg aaatgaatga cgtttattgt ctgagaagcc cttaactggc gagctgagta
     6121 ttaagctgtg ttccggcatc accagcgcaa ctgaccttca gcattacgga taaccagccg
     6181 ggaatatgtt ccctggtcat cttcagtaaa cacattgcgg taagctgttt tgacggcaac
     6241 agcctgttcg cgtgagaaag ggccttcggg caataaacgt gatgtacaac ccggcatatc
     6301 tgttgctccc tgaaagtaaa aagccccggt catgatgacc ggggcctgaa ggagagtgac
     6361 ctgattatca gaaagtcaca ttcagcgtgg cctgaccgtt ataaccttca gcgctgctgc
     6421 cgctgacgct gtgggcataa ccgccctgaa cgcccagggt gatattttcc cggacacggg
     6481 tttccagtcc ggcctgcagg tccagtgacg tgccattccg ggacggtgag aacgtcatgt
     6541 tactgccggc ggcggctgta cccatgctca tgtcaccccg ggagctgaag gtgcggataa
     6601 cagaaggctg tacccaccag ttcaccggca gttcacgcac acggtgtttt gcactgtcgc
     6661 gcagggtgtc acgggatgag gtgccttcac caaagctcat atcgttgtgg ctgcccagac
     6721 ggaaaccggc acgcacatgt tgtgcactgc catgcccaaa cttcacataa ccggcgttat
     6781 cctggccgtc atccagggaa agtccctgcc aggtatactg cagttgtggc tccagcatca
     6841 ggttgtcagt gatactgaag ggcagaccgg tttccagtga gcccagccag ccccagcccc
     6901 gggcgcggaa gtcgttattg tccgatgacg ctttcatgct gtggcgggtt ccctgtgcca
     6961 caatgtcagc ccacaggccg gaggacgtgt gtgtcagatt caggtatccg cccaggctgc
     7021 cggcatcatc ccggaccgtg ccggcgcggg aaccgtcatc atccttaaca tcaacggaag
     7081 aatggcctgc ggcaccatac acccctgtcg tcagagacat accggcaacc tctgttctga
     7141 gcaggtcacc ctccagacgg acgaagccat agctgccgct gctttccggc gtggctccac
     7201 gggcaatacc gccgttgtta tcgtgaccga gatgaccgcc ctgaatgctg agacggacgc
     7261 tgttattttc accgtttaca ccggtctgat ggctgcggga gcctgccaga atccggtcat
     7321 agtccattgc ctgtgtcagc atggatgtat acagggggac ttcagcacga taagcatttt
     7381 cactgcgcag gtaccagtct tcatcgctgt cacggttcag ggtgtagtta aaggcgccgg
     7441 cctgaagcgg gcgactcagg gcaaacgcac cttcttctgt ggtagcgcca ttctgtgcat
     7501 ccacaacccg gataccctgt ccggtggttg ccaccccgag gttactgttt ccgacatttg
     7561 taaacgcaag ccaggttttg ccggttgcct gaccaccatt aatcaccagc tggtcagagg
     7621 cattgctgcc atcaaggcga acacgcatat tgatagtgcc accctgaccg gtgaggttac
     7681 tggttgtcag tttatggaac gttaccgggg cgttgccttt tgcaacagca cggctcagta
     7741 cggcatccgg tgttgtctga ttgtcaaagt aaatctcccc tgcgttaaca atatcgccgg
     7801 tactgaccac atcaccattc agtaccatgc tggcgccttt actgagctgt gttctgccac
     7861 tcagacttcc cccggcagcc agcatcagtg ttccacgatg tccgacagtg gtgttgctgg
     7921 cctgtccccc ggcattaacc gtaaagctgc cgccattttc cagcagcagg ccgctggcct
     7981 gaccggatat gccatcaata ctgaacttac cgctggcatt ggtcccctca acagtggcac
     8041 cactgtctgc aatcagggca ccaccggatg tcatggtgac acctgttgcc ttaccaccgg
     8101 cagacactgc cagggttccg ccgtcatcca cccgtgtttt ctgcgctgaa tggccttcca
     8161 gtacatccag gcgaccgccg gactccagaa caacgccgtc agccttaccg ttttccaccg
     8221 tgaaattgcc cagacggttg ctgccaagaa ccgttgccgc agtactgg
//
LOCUS       DAHHTG010000171         8247 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.174, whole genome shotgun sequence.
ACCESSION   DAHHTG010000171 DAHHTG010000000
VERSION     DAHHTG010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8247)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8247)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8247
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.174"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            complement(<1..186)
                     /gene="ydfK"
                     /locus_tag="NBI74_004097"
     CDS             complement(<1..186)
                     /gene="ydfK"
                     /locus_tag="NBI74_004097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416062.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock protein YdfK"
                     /protein_id="HCD1934347.1"
                     /translation="MKSKDTLKWFPAQLPEVRIILGDAVVEVAKQGRPINTRTLLDYI
                     EGNIKKKSWLDNKELLQT"
     gene            complement(255..368)
                     /locus_tag="NBI74_004098"
     CDS             complement(255..368)
                     /locus_tag="NBI74_004098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1934348.1"
                     /translation="MNSILIITSLLIIFSIFSHALIKLGIGISNNPDKTDV"
     gene            972..2255
                     /gene="ydfJ"
                     /locus_tag="NBI74_004099"
                     /pseudo
     CDS             972..2255
                     /gene="ydfJ"
                     /locus_tag="NBI74_004099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000582800.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MHS family MFS transporter YdfJ"
     gene            2344..3804
                     /locus_tag="NBI74_004100"
     CDS             2344..3804
                     /locus_tag="NBI74_004100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HCD1934349.1"
                     /translation="MGNHLLSAKATLPVYDRNNLAPRIVHLGFGAFHRAHQGVYADIL
                     ATEHFSDWGYYEVNLIGGEQQIADLQQQDNLYTVAEMSADAWTARVVGVVKKALHVQI
                     DGLETVLAAMCEPQIAIVSLTITEKGYFHSPATGQLMLDHPMVVADVQNPHQPKTATG
                     VIVEALARRKAAGLPAFTVMSCDNMPENGHVMRDVVTSYAQAIDVKLAQWIEDNVTFP
                     STMVDRIVPAVTEDTLAKIEQLTGVRDPAGVACEPFRQWVIEDNFVAGRPEWEKAGVE
                     LVSDVLPYEEMKLRMLNGSHSFLAYLGYLAGYQHINDCMEDEHYRHAAYTLMLQEQAP
                     TLKVQGVDLQDYANRLIERYSNPALRHRTWQIAMDGSQKLPQRMLDSVRWHLAHDSKF
                     DLLALGVAGWMRYVGGVDEQGNPIEISDPLLPVIQKAVQSSAEGTARVQSLLAIKAIF
                     GDDLPDNSLFTTKVTEAYLSLLAHGAKATVAKYSVK"
     gene            complement(3840..4043)
                     /gene="ydfZ"
                     /locus_tag="NBI74_004101"
     CDS             complement(3840..4043)
                     /gene="ydfZ"
                     /locus_tag="NBI74_004101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenium delivery protein YdfZ"
                     /protein_id="HCD1934350.1"
                     /translation="MTTYDRNRNAITTGSRVMVSGTGHTGKILSIDTEGLTAEQIRRG
                     KTVVVEGCEEKLAPLDLIRLGMN"
     gene            complement(4220..4906)
                     /gene="ydfH"
                     /locus_tag="NBI74_004102"
     CDS             complement(4220..4906)
                     /gene="ydfH"
                     /locus_tag="NBI74_004102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YdfH"
                     /protein_id="HCD1934351.1"
                     /translation="MTVETQLNPTQPVNQQIYRILRRDIVHCLIAPGTPLSEKEVSVR
                     FNVSRQPVREAFIKLAENGLIQIRPQRGSYVNKISMAQVRNGSFIRQAIECAVARRAA
                     SMITESQCYQLEQNLHQQRIAIERKQLDDFFELDDNFHQLLTQIADCQLAWDTIENLK
                     ATVDRVRYMSFDHVSPPEMLLRQHLDIFSALQKRDGDAVERAMTQHLQEISESVRQIR
                     QENSDWFSEE"
     gene            complement(4995..5741)
                     /gene="ydfG"
                     /locus_tag="NBI74_004103"
     CDS             complement(4995..5741)
                     /gene="ydfG"
                     /locus_tag="NBI74_004103"
                     /EC_number="1.1.1.298"
                     /EC_number="1.1.1.381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional NADP-dependent 3-hydroxy acid
                     dehydrogenase/3-hydroxypropionate dehydrogenase YdfG"
                     /protein_id="HCD1934352.1"
                     /translation="MIVLVTGATAGFGECITRRFIQQGHKVIATGRRQERLQELKDEL
                     GDNLYIAQLDVRNRAAIEEMLASLPAEWSNIDILVNNAGLALGMEPAHKASIEDWETM
                     IDTNNKGLVYMTRAVLPGMVERNHGHIINIGSTAGSWPYAGGNVYGATKAFVRQFSLN
                     LRTDLHGTAVRVTDIEPGLVGGTEFSNVRFKGDDGKAEKTYQNTVALTPEDVSEAVWW
                     VSTLPAHVNINTLEMMPVTQSYAGLNVHRQ"
     gene            5878..7923
                     /gene="dcp"
                     /locus_tag="NBI74_004104"
     CDS             5878..7923
                     /gene="dcp"
                     /locus_tag="NBI74_004104"
                     /EC_number="3.4.15.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-dipeptidase Dcp"
                     /protein_id="HCD1934353.1"
                     /translation="MTTMNPFLVQSTLPYLAPHFDQIANHHYRPAFDEGMQQKRAEIA
                     AIALNPQTPDFNNTILALEQSGELLTRVTSVFFAMTAAHTNDELQRLDEQFSAELAEL
                     ANDIYLNGELFARVDAVWQRRESLGLDSESIRLVEVIHQRFVLAGAKLEQADKAKLKV
                     LNTEAATLTSQFNQRLLAANKSGGLVVNDIAQLAGMSEQEIALAAEAAREKGLDNNWL
                     IPLLNTTQQPALAELRDRATREKLFTAGWRRAEKNDANDTRAIIQRLVEIRAQQAKLL
                     GFPHYAAWKIADQMAKTPEAALNFMREIVPAARQRASDELASIQAVIDKQQGGFSAQP
                     WDWAFYAEQVRREKFDLDEAQLKPYFELNTVLNEGVFWTANQLFGIKFVERFDIPVYH
                     PDVRVWEIFDHNGVGLALFYGDFFARDSKSGGAWMGNFVEQSTLNETHPVIYNVCNYQ
                     KPAAGEPALLLWDDVITLFHEFGHTLHGLFARQRYATLSGTNTPRDFVEFPSQINEHW
                     ATHPQVFARYARHYQSGTAMPDELQQKMRNASLFNKGYEMSELLSAALLDMRWHCLEE
                     NEAMQDVDDFELRALVAENMDLPAIPPRYRSSYFAHIFGGGYAAGYYAYLWTQMLADD
                     GYQWFVEQGGLTRENGRRFREAILSRGNSEDLERLYRQWRGKAPQIMPMLQHRGLNV"
     gene            8072..8176
                     /locus_tag="NBI74_004105"
                     /pseudo
     CDS             8072..8176
                     /locus_tag="NBI74_004105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310172.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00156 family protein"
BASE COUNT         2101 a         1840 c         2076 g         2230 t
ORIGIN      
        1 tgtttgtaat aattctttgt tatccagcca tgattttttc tttatgtttc cttcaatgta
       61 atcaagcaat gttctggtat tgataggtct tccctgtttt gctacttcca ctacagcatc
      121 ccctaggata attcttactt caggaagctg cgcagggaac cactttaggg tgtcttttga
      181 tttcatgaag atattcctta aaatattatt gattttcatt gcgatattgt atgtctgatt
      241 caggatatgt tgacttatac atcggttttg tctgggttat tggatatgcc aatccctaat
      301 tttattaggg catgactaaa aatgctgaat atgataagga gagaagtgat tatcagtatg
      361 ctgttcatat agcctcgaat tagtaatgtg ttatatatga tatagttgac aatttttatc
      421 ttgggtgttc ttaaagttcg tagataaaca ttgtcgtttc aggtatacag gaatgctaac
      481 aggtggcagc aaaaatcagg cggtttatgg cgcaagctga agcggcaact gcaaactatc
      541 ttatgcagag actctacacg gattgggttt aaaagtatac atagataaca gtttttatct
      601 gaaaaagaaa aatatcaagg tgatatagcc tatatgcctt tgatgcggag gaatgaatgt
      661 gatgggagtg atgtatctga atagttgaaa aaccgcagac acgccttatg caagaacgtg
      721 ctgcggttgg ctggtaaatt tttcgatagt gtgagtattg aatgatttcc agccgttctt
      781 gattttacgc ataaatccat gaaaaaacta cttatctgtt ggggagtttt tttggggcat
      841 atatgggaca gaaataggcc ccagatagac actgagatcg aacttaggat gctttttaaa
      901 aaatgcaact atctgaaaaa acctagaaaa cgccaaggaa accacaggat gggaaaaaac
      961 acctgtgaat tatggatttc cagttatatt cgctcggcgc agcgttagtg tttcatgaaa
     1021 tattttttcc tgaatcatca acggcaatgg cgttaattct ggcaatggga acctatggtg
     1081 caggttatgt ggcacgtatt gtcggagcat ttattttcgg caaaatgggc gacagaatag
     1141 ggcgtaaaaa agtgctcttt attaccatca ccatgatggg gatctgtacc accttaattg
     1201 gtgtgttacc gacttatgca caaattggtg tttttgcccc catcttgctg gtgacgctgc
     1261 gtattattca ggggttgggt gcaggtgcgg aaatttccgg tgccggtacg atgctggcgg
     1321 aatatgcgcc aaaaggtaag cgcggaatta tctcctcatt tgtggctatg ggaactaact
     1381 gcggaacctt gagcgcaacg gcaatctggg cctttatgtt cttcattctc agtaaagagg
     1441 aactgctggc gtggggatgg cgtatacctt tcctggcgag cgttgtcgtg atggtctttg
     1501 ctatctggtt gcgtatgaat ctgaaagaaa gcccggtctt tgagaaggtt aacgacagca
     1561 accaaccgac agcaaaacct gcacctgctg gtagcatgtt ccagagcaaa tccttctggc
     1621 tggcaacagg gctgcgtttt ggtcaggctg gtaactcagg tttaattcag actttccttg
     1681 caggctattt agtgcagacg ttattgttta acaaagcaat tccaacagat gcattgatga
     1741 tcagttcgat tctcggcttt atgaccattc cgttccttgg ttggttatcc gataaaattg
     1801 gtcgccggat cccgtatatt attatgaata cctctgcgat tgtgctggca tggccaatgc
     1861 tttctatcat cgtagataaa agctatgccc cgagcaccat tatggttgca ctgattgtga
     1921 ttcataactg tgcggtgctt ggattatttg ctctggaaaa cattaccatg gcagaaatgt
     1981 tcggctgtaa aaaccgcttt acccgaatgg ctatttctaa agaaattggt ggtcttatcg
     2041 cttccggttt tggtcctatc ctggcgggga ttttctgcac catgacggaa tcctggtatc
     2101 cgatcgccat tatgatcatg gcatattctg tgattggttt aatctctgcg ctgaaaatgc
     2161 cagaagtgaa agaccgtgat ttaagtgcgc tgcaagacgc cgcagaagat caaccacatg
     2221 ttgtaagagc tgcgcaaccg tccagaagtc tgtaaatcct caatcccttc tcttaccgga
     2281 gaggggattt ttattcatat aaaaaaacat gtagcttgct atatttaatt ttaaggaatt
     2341 gttatgggaa atcatttgtt atcagcaaaa gcgacgctcc ctgtttatga tcgtaataac
     2401 ctggccccaa gaattgttca tttaggcttt ggtgcatttc accgtgcgca tcagggcgtg
     2461 tatgccgata ttcttgctac ggaacatttc agtgactggg gatattatga ggtcaactta
     2521 atcggcggcg aacagcaaat tgccgattta caacagcaag ataatcttta taccgttgcg
     2581 gaaatgtcgg ctgatgcgtg gacggctcgc gtcgttggcg tcgttaaaaa agccttacac
     2641 gtacaaatag acggcttaga aaccgtgttg gctgcgatgt gtgaaccgca aatcgcgatt
     2701 gtctctctga caatcaccga aaaagggtat ttccactctc cggcgaccgg acagttaatg
     2761 ctcgatcacc cgatggtcgt tgccgacgtg caaaatcccc accagccgaa aactgcaaca
     2821 ggggtgattg ttgaggcgct ggctcgccgt aaagcggcag gacttcccgc atttaccgtc
     2881 atgtcatgtg acaacatgcc agaaaacggt catgttatgc gtgacgttgt cacttcctat
     2941 gcgcaagcca ttgatgtaaa actggcacaa tggatcgaag ataacgtgac tttcccatca
     3001 acaatggtgg accgtattgt gcccgcagtg acagaggata cgctggcgaa aatcgaacaa
     3061 cttaccggtg tgcgcgatcc ggcaggcgtt gcctgtgaac ctttccgcca gtgggtaata
     3121 gaagataact ttgttgccgg acgtccggaa tgggaaaaag cgggagtcga actggttagt
     3181 gatgtgctgc cttatgaaga gatgaagttg cgcatgctca atggcagtca ttcattcctg
     3241 gcgtatctgg gctatcttgc cggatatcag cacattaatg actgcatgga ggatgaacat
     3301 tatcgtcatg cggcgtatac cttaatgttg caggaacaag cgccgacgct gaaagtgcag
     3361 ggcgttgatt tgcaagatta cgctaaccga ttaattgaac gctatagcaa cccggcgtta
     3421 cgtcatcgaa cctggcagat tgcgatggat ggcagccaga aattgccaca gcggatgttg
     3481 gattctgttc gctggcatct ggcgcatgac agcaagttcg atctgctggc gctgggcgtc
     3541 gcgggttgga tgcgttatgt cggtggtgtt gatgaacagg gaaatccgat agaaatcagt
     3601 gacccactgt tacctgttat tcagaaggca gtacaaagta gtgctgaagg gacagcgcgc
     3661 gtgcagtcat tgttggcgat taaggcgata tttggtgatg atttgccaga taatagcttg
     3721 tttacgacta aagtgacgga agcgtacttg tctttattag cgcatggcgc gaaagcgaca
     3781 gtggcgaaat attccgtgaa gtaaaaaaag caatgccgcc atcggcggca ttcacacgct
     3841 tagttcatgc cgagacgaat caggtccagt ggtgccagtt tctcttcaca accttcaaca
     3901 actacggttt ttccgcggcg gatttgctca gcggtcagac cttcagtatc aatcgacagg
     3961 atcttgccag tgtgaccggt gccgctaacc ataacacggc tgccagtggt gattgcgtta
     4021 cggttacgat cgtaggtcgt cataggtgtt ttctcctttc tgatttacag tcgaagccca
     4081 cgtaacgggc gcaatataaa tactcctgtc gagtgaaata ttttttgttt ttgatcaagc
     4141 tcacatcttt tttgatgcat gcatgagcca taagtcgggg ggaaagactc tcaccccgga
     4201 tgggatgaga ggaaatgaat tactcttcgc taaaccagtc gctgttttcc tggcggatct
     4261 ggcgcacgga ttcgctgatt tcctgcaaat gttgcgtcat tgcacgttct accgcatcgc
     4321 catcacgttt ttgcagggca gagaaaatat caagatgctg gcgtaacagc atttctggtg
     4381 gagaaacgtg gtcgaagctc atatagcgca cgcgatcaac ggtcgctttc aggttctcaa
     4441 tggtatccca cgccagttga cagtcggcaa tctgcgtcag gagttgatgg aagttgtcat
     4501 caagttcaaa aaaatcatcc agttgcttgc gctcaatggc aatgcgttgc tggtgaagat
     4561 tttgttccag ttggtagcac tggctttcgg taatcatgct cgccgcccga cgcgccaccg
     4621 cgcactcaat ggcctgacgg ataaaactgc cgttgcgcac ctgggccatg gaaattttgt
     4681 tgacgtagct gccacgctgc ggacgaattt gaatcagacc gttttccgcc agtttaataa
     4741 aggcttcacg aaccggctgg cgtgacacat tgaaacgaac agaaacttct ttttccgaca
     4801 acggtgtgcc tggtgcaatc aggcaatgga caatgtcgcg gcgaagaata cgataaatct
     4861 gctgattgac aggctgtgtg ggattaagtt gcgtttcgac ggtcattcgt tcttgcttgt
     4921 gagtgagtta actgcatgag tctaccactt ttttgtgaca agcaataacc cggcagttac
     4981 gccgggtata aaaattactg acggtggaca ttcagtccgg catagctttg ggtaaccggc
     5041 atcatttcca gtgtattgat attgacgtga gcaggcagcg ttgacaccca ccagacggct
     5101 tcgctgacat cttctggcgt caatgcaacg gtattttgat aggttttttc cgctttaccg
     5161 tcatcgcctt taaagcggac attggaaaac tcggtgccac ccaccagacc cggttcgatg
     5221 tcggtgacgc gcaccgccgt accatgcagg tccgtacgca gattcaggct aaattgacga
     5281 acaaacgctt tcgtcgcacc gtaaacgtta ccaccggcat acggccagct acctgccgtt
     5341 gagccaatgt taataatatg accatgatta cgttcaacca tacccggtaa gacggcgcgc
     5401 gtcatatata ccaggccttt gttgttggta tcaatcatcg tttcccagtc ttcaatgctg
     5461 gctttatgag caggctccat gcccaacgcc aggccggcat tgtttaccag gatatcaata
     5521 ttgctccact cggcagggag cgatgccagc atctcttcaa tagcggcgcg gttgcgaacg
     5581 tccagttggg cgatatacag attatctccc agttcgtctt ttaactcctg caaccgctcc
     5641 tggcgacgac cagtggcgat aactttatgc ccttgttgaa taaaacgacg agtaatgcat
     5701 tcaccaaaac ctgccgttgc tccagttact aaaacgatca tctcactgtt cctcaacgat
     5761 tttgtgtgtc aataacatag cacgtgatgc ttaagagcgg gtaatactgg tttgcaagat
     5821 tgcactccgt tatcagtaag cctacgctat ggaacacctc gcgttcagga gaagaaaatg
     5881 acaacaatga atcctttcct tgtgcaaagc acactgccgt atctggctcc ccattttgat
     5941 caaattgcca atcatcacta tcgcccggca ttcgatgagg gaatgcagca aaagcgggca
     6001 gaaattgctg ccatcgcgct taacccgcaa acacctgatt tcaacaatac tattctggca
     6061 ctggaacaaa gcggagaatt acttacccgc gttaccagcg tcttttttgc gatgactgcg
     6121 gcgcatacca atgatgaatt acagcgtctt gatgaacagt tttccgctga actggcggaa
     6181 cttgcgaatg atatctatct gaacggggaa ttattcgcgc gggtagatgc tgtctggcaa
     6241 cgccgtgaat ccctggggct tgatagtgaa tccatccgcc tggtggaggt gattcatcaa
     6301 cgttttgtcc ttgccggagc caaacttgag caagctgata aagcaaaatt aaaagtactg
     6361 aatacagaag ctgcgaccct gaccagccag tttaaccagc ggttactggc agcaaataaa
     6421 tccggcggtc tggttgtgaa cgatatcgcg cagctggcag gaatgagtga acaagagatt
     6481 gcgctggcgg cagaggcggc tcgcgagaaa ggtctggata acaactggct gattccgctg
     6541 ctgaatacca cccagcaacc agcgcttgct gaactgcgcg atcgcgcgac gcgtgaaaaa
     6601 ctgtttactg cgggctggag gcgagcggaa aaaaatgatg ccaatgatac ccgcgctatc
     6661 attcaacgtc tggtggagat ccgcgcgcag caggcgaaac tgcttggttt tcctcattat
     6721 gccgcatgga aaatcgccga tcagatggca aaaacgccag aagcagcgct caactttatg
     6781 cgggaaattg ttccagcggc gcgtcaacgt gcgagcgatg aattagcctc catacaggcg
     6841 gttatcgata agcagcaggg cgggtttagc gcgcagccgt gggactgggc attttatgcc
     6901 gagcaggtaa gacgtgagaa attcgatctc gatgaagcgc agctcaagcc atattttgaa
     6961 ttaaacacgg tgttgaatga aggcgtattc tggactgcga atcagctctt cggtattaag
     7021 tttgtcgaac gttttgatat tcctgtctac catcctgacg ttcgtgtgtg ggaaattttt
     7081 gatcataatg gcgtggggct ggcgttattt tacggtgatt tcttcgcccg tgattcaaaa
     7141 agcggcggcg catggatggg caattttgtt gagcaatcaa cgctgaatga aacgcacccg
     7201 gtaatttata acgtctgcaa ttatcagaaa cccgctgccg gtgagcctgc gttgttactc
     7261 tgggatgatg taataacctt attccatgaa tttggtcata cgctgcacgg cctttttgcc
     7321 cgccagcgtt atgccacgct ttccggcacc aacacgccgc gtgattttgt cgaatttccg
     7381 tcgcaaatca acgaacactg ggcaacacat ccgcaggtat tcgctcgcta cgcccggcat
     7441 tatcagagcg ggaccgcaat gcctgacgaa cttcaacaga aaatgcgtaa tgccagcctg
     7501 ttcaataaag ggtatgagat gagcgaactg cttagcgccg cacttctcga tatgcgctgg
     7561 cattgcctgg aagaaaacga agcaatgcag gatgtcgatg atttcgaatt gcgggcgctg
     7621 gtggcggaaa atatggatct tcctgctata ccgccacgct atcgcagcag ttatttcgcc
     7681 catatttttg gtggcggata tgccgcaggt tattacgctt atctgtggac gcaaatgttg
     7741 gccgatgatg gttaccagtg gtttgttgag cagggcggat taacgcgtga aaatgggcgg
     7801 cgttttcgcg aggcgattct ttccagaggt aacagcgaag atctggaacg cctgtaccga
     7861 caatggcgcg gtaaggcacc tcagattatg ccgatgttgc aacatcgtgg attgaacgta
     7921 taaacaccac caatggctgg cctggattta tcgccagacc agcctcgtta tagcagtaat
     7981 tgcagccgct gggcgagggc aaacttcacc gctaatgcga atgccgtttc gcagtcgcca
     8041 gcaaaattac tttttttgta tactcttccg ttataataat tttcaaattc agccggacca
     8101 gatgatcaac attagcggca gtctggataa gaaaagcgcg ccggccgtcg ttcgggtcac
     8161 tcatttgcag aaataaaaat aaattagcct gatggcctga actactgggc cattagggta
     8221 ttgggttatg ctatttgtca tcctttt
//
LOCUS       DAHHTG010000172         8202 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.175, whole genome shotgun sequence.
ACCESSION   DAHHTG010000172 DAHHTG010000000
VERSION     DAHHTG010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8202)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8202)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8202
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.175"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            591..767
                     /locus_tag="NBI74_004106"
     CDS             591..767
                     /locus_tag="NBI74_004106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016158417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1934354.1"
                     /translation="MKKTFTFSLLAIVFASLLSACVPHHHHRHSDGPRGPAPSGKPMP
                     PSNGGPNHGSHGRY"
     gene            complement(804..2723)
                     /gene="dhaR"
                     /locus_tag="NBI74_004107"
     CDS             complement(804..2723)
                     /gene="dhaR"
                     /locus_tag="NBI74_004107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001467850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase operon
                     transcriptional regulator DhaR"
                     /protein_id="HCD1934355.1"
                     /translation="MSGAFNNDGRGISPLIATSWERCNKLMKRETWNVPHQAQGVTFA
                     SIYRRKKAMLTLGQAALEDAWEYMAPRECALLILDETACILSRNGDPQTLQQLSVLGF
                     NDGTYCAEGIIGTCALSLAAISGQAVKTMADQHFKQALWNWAFCATPLFDSKGRLTGT
                     IALACPVEQTTAADLPLTLAIAREVGNLLLTDSLLAETNRHLNQLNALLESMDDGVIS
                     WDEQGNLQFINAQAARVLRLDATASQGRAITELLTLPAVLQQAIKQAHPLKHVEATFE
                     SQHQFIDAVITLKPIIETQGTSFILLLHPVEQMRQLMTSQLGKVSHTFAHMPQDDPQT
                     RRLIHFGRQAARSSFPVLLCGEEGVGKALLSQAIHNESERAAGPYIAVNCELYGDAAL
                     AEEFIGGDRTDNENGRLSRLELAHGGTLFLEKIEYLAVELQSALLQVIKQGVITRLDA
                     RRLIPIDVKVIATTTADLAMLVEQNRFSRQLYYALHAFEITIPPLRMRRGSIPALVNN
                     KLRSLEKRFSTRLKIDDDALARLVSCAWPGNDFELYSVIENLALSSDNGRIRVSDLPE
                     HLFTEQATDDVSATRLSTSLSFAEVEKEAIINAAQVTGGRIQEMSALLGIGRTTLWRK
                     MKQHGIDAGQFKRRG"
     gene            2951..4021
                     /gene="dhaK"
                     /locus_tag="NBI74_004108"
     CDS             2951..4021
                     /gene="dhaK"
                     /locus_tag="NBI74_004108"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309732.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HCD1934356.1"
                     /translation="MKKLINDVQDVLDEQLAGLAKAHPSLTLHQDPVYVTRADAPVAG
                     KVALLSGGGSGHEPMHCGYIGQGMLSGACPGEIFTSPTPDKIFECAMQIDGGEGVLLI
                     IKNYTGDILNFETATELLHDSGVKVTTVVIDDDVAVKDSLYTAGRRGVANTVLIEKLV
                     GAAAERGDSLDACAELGRKLNNQGHSIGIALGACTVPAAGKPSFTLADNEMEFGVGIH
                     GEPGIDRRPFSSLDQTVDEMFDTLLENGSYHRTLRLWDYQQGSWQEEPQTKQPLQSGD
                     RVIALVNNLGATPLSELYGVYNRLTTRCQQAGLTIERNLIGAYCTSLDMTGFSITLLK
                     VDDETLALWDAPVHTPALNWGK"
     gene            4032..4664
                     /gene="dhaL"
                     /locus_tag="NBI74_004109"
     CDS             4032..4664
                     /gene="dhaL"
                     /locus_tag="NBI74_004109"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase ADP-binding subunit
                     DhaL"
                     /protein_id="HCD1934357.1"
                     /translation="MSLSRTQIVNWLTRCGDIFSTESEYLTGLDREIGDADHGLNMNR
                     GFGKVVEKLPAIADKDIGFILKNTGMTLLSSVGGASGPLFGTFFIRAAQTTQARQSLT
                     LEELYQMFRDGADGVISRGKAEPGDKTMCDVWVPVVESLRQSCEQNLSVPAALDAASS
                     IAESAAQSTITMQARKGRASYLGERSIGHQDPGATSVMFMMQMLALAAKE"
     gene            4675..6093
                     /gene="dhaM"
                     /locus_tag="NBI74_004110"
     CDS             4675..6093
                     /gene="dhaM"
                     /locus_tag="NBI74_004110"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415716.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaM"
                     /protein_id="HCD1934358.1"
                     /translation="MVNLVIVSHSSRLGEGVGELARQMLMSDNCKIAIAAGIDDPQNP
                     IGTDAVKVMEAIESVADADHVLVMMDMGSALLSAETALELLAPEIAAKVRLCAAPLVE
                     GTLAATVSAASGADIDKVIFDAMHALEAKREQLGLPSSDTEISDTCPPYDEEARSLAV
                     VIKNRNGLHVRPASRLVYTLSTFNADMLLEKNGKCVTPESINQIALLQVRYNDTLRLI
                     AKGPEAEEALIAFRQLAEDNFGETEEVAPPTLRPVPPVSGKAFYYQPVLCTVQAKSTL
                     TVEEEQERLRQAIDFTLLDLMTLTAKAETSGLDDIAAIFSGHHTLLDDPELQAAASEL
                     LQHEHCTAEYAWQQVLKELSQQYQQLDDEYLQARYIDVDDLLHRTLVHLTQTKEELPQ
                     FNSPTILLAENIYPSTVLQLDPAVVKGICLSAGTPLSHSALIARELGIGWICQQGEKL
                     YAIQPEETLTLDVKTQRFNRQG"
     gene            6413..8110
                     /gene="treA"
                     /locus_tag="NBI74_004111"
     CDS             6413..8110
                     /gene="treA"
                     /locus_tag="NBI74_004111"
                     /EC_number="3.2.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
                     /protein_id="HCD1934359.1"
                     /translation="MKSPAPSRPQKMALIPACIFLCFAALSVQAEETSVTPQPPDILL
                     GPLFNDVQNAKLFPDQKTFADAVPNSDPLMILADYRMQQNQSGFDLRHFVNVNFTLPK
                     EGEKYVPPEGQSLREHIDGLWPVLTRSTENTEKWDSLLPLPKPYVVPGGRFREVYYWD
                     SYFTMLGLAESGHWDKVADMVANFAHEIDTYGHIPNGNRSYYLSRSQPPFFALMVELL
                     AQHEGDAALKQYLPQMQKEYAYWMDGVENLQAGQQEKRVVKLQDGTLLNRYWDDRDTP
                     RPESWVEDIATAKSNPNRPATEIYRDLRSAAASGWDFSSRWMDNPQQLNTLRTTSIVP
                     VDLNSLMFKMEKILARASKAIGDNAMANQYETLANARQKGIEKYLWNDQQSWYADYDL
                     KSHKVRNQLTAAALFPLYVNAAAKDRASKMATATKTHLLQPGGLNTTSVKSGQQWDAP
                     NGWAPLQWVATEGLQNYGQKEVAMDISWHFLTNVQHTYDREKKLVEKYDVSTTGTGGG
                     GGEYPLQDGFGWTNGVTLKMLDLICPKEQPCDNVPATRPLSESTTQPLKQKEAEPTP"
BASE COUNT         2126 a         2098 c         1984 g         1994 t
ORIGIN      
        1 ttttctttag taacagaagg ttaactatca aatatttttg ttgggctatg tgggctaaca
       61 gttttcatat ttacgtgttt ttttattctt ctccggtttc cgtttcagat accggagtaa
      121 acaacagcgt ataagcccag ggacgtgcat gcttgaggtg tctcagccgt ggcatacgta
      181 tcagccatcc ctccacgctg gtggactttt tgagcattcc agcctcatga aggattctgc
      241 cggcagtatc cttgctgaga ggagtagaac ctacgccctt cgcattacga atgcgagcta
      301 actccaaaat gagtacgaac atgagtacaa aaaaaatata tcattaaatg tagtataaat
      361 ttcaatcaga tacagaaaaa attacaattc aacgtctaat tccgctaata ctactctggc
      421 ggttaataac gagccattat gtgagttgca cgtaatggct tttttacgcc tgctacgcgt
      481 cttctttcaa ttctgagtta ccaaaactca taatcagact cctgacaata ttaacaatgc
      541 agcttttatg tgataacaat cacagtgcat taaccgttca agagtttgtc atgaaaaaaa
      601 catttacctt cagcctgtta gcaattgtct ttgcaagcct gttgagtgct tgtgttcccc
      661 accatcatca ccgccacagc gatggccctc gtggtcctgc gccctccggt aagccaatgc
      721 caccttcaaa tggtggacca aatcacggca gtcatggtcg ctattaatta gtaattcata
      781 gcgggaaagt acatactctc ccgctatcca cggcgcttaa actgccctgc atcaatgcca
      841 tgttgcttca ttttccgcca cagcgtagtg cggccgattc caagtaaagc cgacatttcc
      901 tgaatgcgac cgcctgtgac ttgggctgcg ttaataattg cctctttttc aacttccgca
      961 aatgacagac tggtggaaag gcgggtggcg ctgacatcat ctgtcgcctg ctcggtaaac
     1021 agatgttccg gcaaatcact gacgcgaatg cgcccattat cactactcag agcaagattc
     1081 tcgatgacgc tgtaaagttc aaaatcgttg ccaggccatg cacaggaaac caggcgagcg
     1141 agggcatcgt catcaatttt cagccgcgta gagaagcgtt tttcaaggct gcgtaatttg
     1201 ttattcacca gcgccggaat gctgccacgc cgcatacgca gtggcgggat ggtaatttca
     1261 aatgcatgca gcgcgtaata cagctggcga ctaaaacgat tttgttccac cagcattgcg
     1321 aggtccgcgg tcgttgtggc aatcactttg acatcaatgg gtattaaacg ccgcgcatcc
     1381 agtcgcgtga taacaccctg cttgataacc tgaagcaaag cagactgtaa ctccaccgcc
     1441 agatattcaa tcttttcaag aaacagcgtg ccgccgtgcg ccagttccag ccgactcaga
     1501 cggccatttt cattatccgt gcgatcgcca ccaataaatt cttccgccag cgccgcatca
     1561 ccatataact cacaattgac ggcgatataa ggccccgctg cacgctcgct ttcattatga
     1621 attgcctgac ttagcagtgc cttgcccacg ccctcttctc cacaaagcag gacaggaaag
     1681 ctactgcgcg ccgcctggcg accaaaatga atcaagcggc gggtttgcgg atcgtcctgt
     1741 ggcatatgag cgaaggtatg gctgactttt cctaattgac tggtcattaa ctgccgcatc
     1801 tgttccacag gatggagcaa taaaataaag ctggttccct gcgtttctat tatcggttta
     1861 agggttatca ccgcatcaat aaactgatgc tgactttcaa aggttgcttc tacgtgtttg
     1921 agcggatgtg cctgttttat tgcttgttgc aatacggcgg gtaacgttaa gagttcagtg
     1981 attgcccttc cctgacttgc cgtcgcgtca aggcgcaaga ctcgcgccgc ctgggcgttg
     2041 ataaattgca aattaccctg ctcgtcccag ctaatcacgc catcatccat actttctaac
     2101 agggcattaa gttgatttaa atgacggtta gtttcagcga gcaaactgtc cgtcagcagt
     2161 aaatttccga cctcgcgtgc gattgccaac gtcaacggca aatcagctgc ggtagtttgc
     2221 tcaaccggac acgccagcgc tattgttccc gtcaatcggc ccttgctgtc aaacaacggc
     2281 gttgcgcaaa aggcccagtt ccagagtgcc tgtttgaaat gttgatcggc catcgttttc
     2341 acggcctgac cagagatagc cgctaacgat agcgcacaag taccaataat tccctcggcg
     2401 caatacgtgc cgtcattgaa tcccagtaca cttagctgct gcaaggtttg cggatcgcca
     2461 ttacggctga gaatgcaggc ggtttcatcg aggataagca acgcacactc tcgcggtgcc
     2521 atatattccc aggcatcttc cagcgcagcc tgcccgagcg tcagcatcgc tttcttacgc
     2581 cgataaatag aagcaaatgt cacgccctgg gcctggtgtg gtacgttcca tgtctcccgt
     2641 ttcatcagct tattgcatct ctcccaggag gttgcaatta agggagatat gccccgacca
     2701 tcgttgttaa aagcgccact catatcccgc atcccttatg acaaaaggtg ttccggctcg
     2761 caattttaag tattaataca cgtaaaagtt tcaatatgga acactcatta aagcatatgt
     2821 ttcactatga aacataattt gggctaattt tcttttccct tgcctgatgc acaatggatt
     2881 catcggcagt cagtggtcgc cgtgtcgttg aacatcatcc atgccctacc gtaattgctg
     2941 gagcaaaata atgaaaaaat tgatcaatga tgtgcaagac gtactggacg aacaactggc
     3001 aggactagcg aaagcgcatc catcgctgac actgcatcag gatccggtgt atgtcacccg
     3061 agctgatgcc cccgttgcag gaaaagtcgc cctgctgtcg ggtggcggca gcggacacga
     3121 gccgatgcat tgtggttata tcggtcaggg gatgctttcg ggggcctgtc cgggcgaaat
     3181 tttcacctca ccgacgcccg ataaaatctt tgaatgcgcc atgcaaattg atggcggcga
     3241 aggtgtactg ttgattatca aaaattacac cggcgatatt cttaacttcg aaacagcaac
     3301 cgagttactg cacgatagcg gcgtaaaagt gaccactgtg gtcattgatg acgacgttgc
     3361 agtaaaagac agtctttata ccgccgggcg acgcggcgtt gccaacaccg tattaattga
     3421 aaaactcgta ggtgcagcgg cggagcgtgg cgactcactg gacgcctgtg cggaactggg
     3481 gcgtaagttg aataatcaag gccactcaat aggtatcgct ctcggtgcct gtaccgttcc
     3541 tgccgcgggc aaaccttctt ttaccctggc ggataatgag atggagtttg gcgtcggcat
     3601 tcatggtgag ccaggtattg accgccgccc cttctcttcc cttgatcaaa ccgtcgatga
     3661 aatgttcgac accctgctgg aaaatggctc ataccatcgc actttgcgtt tatgggatta
     3721 tcaacaaggc agctggcagg aagaaccaca aaccaaacaa ccgctccagt ctggcgatcg
     3781 ggtgattgcg ctggttaaca atcttggcgc aactccgctt tctgagctgt acggcgtcta
     3841 taaccgcctg accacacgtt gccagcaagc gggattgact atcgaacgta atttaattgg
     3901 cgcgtactgc acctcactgg atatgaccgg tttctcaatc accttactga aagttgatga
     3961 cgaaacgctg gcactctggg acgccccggt ccacaccccg gcccttaact ggggtaaata
     4021 aggagaaagc aatgtcactg agcagaactc aaattgttaa ctggctcact cgttgtggcg
     4081 atattttcag caccgagagc gagtatctta ctggactgga tcgcgagatt ggcgacgctg
     4141 accacgggct aaatatgaac cgaggctttg gcaaagtggt ggaaaaactc cctgctatcg
     4201 cagataaaga tatcggtttc attctcaaga ataccggcat gacgctactt tccagcgtcg
     4261 gaggtgccag tggtccgctg ttcggtacct tctttatccg cgccgcacag acaacccagg
     4321 cacggcaaag cctgacgctg gaagagcttt atcagatgtt ccgcgatggc gcggacggcg
     4381 taatcagtcg cgggaaagcc gaacctggcg ataaaaccat gtgtgatgtg tgggtgccgg
     4441 tggtggaatc gttacgtcag tcctgcgagc aaaatctctc tgttccggcg gcgctcgacg
     4501 ctgccagtag catcgccgaa tccgctgcac aaagtacgat tacgatgcaa gcccgcaaag
     4561 gccgcgccag ttatctcggt gaacgcagta ttggtcacca ggatcccggc gcgacctcgg
     4621 tgatgtttat gatgcaaatg ttggcgttag ccgcaaaaga gtaaggaatt ggtgatggta
     4681 aacctggtca tagtttcaca tagcagccga ctgggagaag gtgtcggtga attagcccgt
     4741 cagatgttaa tgagtgataa ttgtaaaatc gccattgccg cgggaattga cgatccacaa
     4801 aatcccattg gtaccgatgc cgtcaaagtg atggaggcca tcgaatctgt tgccgatgcc
     4861 gaccatgtgc tggtcatgat ggatatgggt agcgcattat tgagtgctga aactgcgctg
     4921 gaattgctgg ctcccgagat cgccgcaaaa gtacgtttgt gtgctgcgcc gttggtcgaa
     4981 ggtacactgg cagcaacggt cagcgcggcc tcgggggcgg atatcgacaa agttatcttt
     5041 gacgccatgc atgcgctgga agccaaacgt gaacaactgg gtttaccgtc ctccgacact
     5101 gaaatctctg acacatgtcc tccgtacgat gaagaagccc gttctctggc ggtggtcata
     5161 aaaaaccgta acggcctgca tgtacgtccg gcctcccggc tggtttatac cttatcgaca
     5221 tttaatgccg atatgttgct ggagaaaaac ggcaaatgcg ttacaccgga gagtattaac
     5281 cagattgcgt tactacaagt tcgctataac gatacgctgc gcctgattgc gaaaggacca
     5341 gaagctgaag aggcactgat cgctttccgt cagttggctg aagataactt tggtgaaacg
     5401 gaggaagttg ctccacctac tctgcgtccc gttccgcctg tttcgggtaa agccttttat
     5461 tatcaacctg ttttatgtac ggtacaggca aaatcaaccc tgaccgtgga agaagaacaa
     5521 gaacgattac gccaggccat tgacttcacg ttattagatc tgatgacgtt aacagcgaaa
     5581 gcagaaacca gcgggcttga cgatattgcc gcaatctttt ctggtcacca tacactgtta
     5641 gatgatccgg aactgcaggc ggcggcaagc gaactccttc agcatgaaca ttgcacggcg
     5701 gaatatgcct ggcagcaagt tcttaaagaa cttagccagc aataccaaca gctggatgat
     5761 gaatatctac aggctcgcta tatcgatgtg gacgatcttc tgcatcgcac cctggtccac
     5821 ctgacccaaa cgaaagaaga actcccgcag tttaactcgc caactattct actggcggag
     5881 aacatttatc cctccacagt actgcaactg gatccagcgg ttgtaaaagg tatctgcctt
     5941 agcgccggaa ctccgctatc ccacagtgcc ctaatcgccc gtgaactggg aattggctgg
     6001 atttgccagc agggtgagaa actgtatgcg atacaaccag aagaaacgct aacgctggac
     6061 gttaaaacgc aacgtttcaa ccgtcagggt taaaccaccg ttcggatggc atcattctga
     6121 tgtcatccgg caatttactc tgcactgatg ttttttaact acctgttata tttttcacca
     6181 aattatgatg atttttcttc gctaatcgcg aacccaacgt cgctggctga cgaataaaca
     6241 gatgttattt tatcactcta tttcgccgtt atttatccct ttaattccct tttctaaaat
     6301 gtctgtcagt tcgcagaatg tgatttcgat catgcagcta gtgcgatcct gaactaaggt
     6361 tttctgatac ttgaataccg ttttattcgg tttcgccaaa ggagaatgat tgatgaaatc
     6421 ccccgcacct tctcgcccgc aaaaaatggc gttaattcca gcctgcatct ttttgtgttt
     6481 cgctgcgcta tcggtgcagg cagaagaaac atcggtaaca ccacaaccgc ctgatatttt
     6541 attagggccg ctgtttaatg atgtgcaaaa cgccaaactt tttccggacc aaaaaacctt
     6601 tgccgatgcc gtgccgaaca gcgatccgct gatgatcctt gctgattatc ggatgcagca
     6661 aaaccagagc ggatttgatc tacgccattt cgttaacgtc aatttcaccc tgccgaaaga
     6721 gggcgagaaa tatgttccgc cagaggggca atcactgcgc gaacatattg acggactttg
     6781 gccggtatta acgcgttcta ccgaaaacac cgaaaaatgg gattctctat taccgctgcc
     6841 aaagccttat gtcgtgccag gcggacgttt tcgcgaggta tattactggg acagttactt
     6901 caccatgtta ggacttgccg aaagcggtca ctgggataaa gtcgcggata tggtggccaa
     6961 ttttgctcat gaaatagaca cttacggtca tattcccaac ggcaaccgca gttactattt
     7021 aagccgctcg caaccgccct tctttgccct gatggtagag ttactggcgc agcatgaagg
     7081 cgacgccgcg ttgaaacaat acctgccgca aatgcaaaaa gaatatgctt actggatgga
     7141 cggtgttgaa aacctgcaag ccggacaaca ggaaaaacgc gttgtaaaac ttcaggatgg
     7201 tacccttctc aaccgctact gggacgatcg cgatacgcca cgaccagagt catgggtgga
     7261 agatattgcc actgccaaaa gcaatccgaa tcgacctgcc actgaaattt accgcgacct
     7321 gcgctctgcc gctgcgtctg gctgggattt cagctcgcgc tggatggaca atccgcagca
     7381 gttaaacacc ttacgcacca ccagcatcgt accggtcgat ctgaacagct tgatgttcaa
     7441 aatggaaaaa atcctcgccc gcgccagcaa agctatcgga gataacgcga tggcaaacca
     7501 gtacgaaacg ctggcaaatg cccgtcaaaa agggatcgaa aaatacctgt ggaacgatca
     7561 acaaagctgg tatgccgatt acgacctgaa aagtcataaa gtgcgcaatc agttaaccgc
     7621 ggccgccctg ttcccgctgt acgtcaatgc ggcagcgaaa gatcgcgcca gcaaaatggc
     7681 gacggcgacg aaaacacatc tgctgcaacc aggcggcctg aacaccacgt cggtgaaaag
     7741 tgggcaacaa tgggatgcgc caaacggctg ggcgccgttg cagtgggtcg cgacagaagg
     7801 attacaaaac tacgggcaaa aagaggtagc gatggacatt agctggcact tcctgaccaa
     7861 tgttcagcac acctatgacc gggagaaaaa gctggtggaa aaatatgatg tcagcaccac
     7921 cggaacaggg ggcggcggtg gcgaatatcc attacaggat ggctttggct ggaccaatgg
     7981 cgtgacgctg aaaatgctgg atttgatctg cccgaaagag caaccgtgtg acaatgttcc
     8041 ggcgacgcgt ccactcagtg aatcaacaac acagccgcta aaacaaaaag aggcggaacc
     8101 tacgccttaa ctttactgta tatcgagcaa tgccattacg tgcataaaat tggcattgct
     8161 ctttcttcat cacgaaatac cactctttct acggtctatg cc
//
LOCUS       DAHHTG010000173         8081 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.176, whole genome shotgun sequence.
ACCESSION   DAHHTG010000173 DAHHTG010000000
VERSION     DAHHTG010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8081)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8081)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8081
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.176"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            64..1230
                     /locus_tag="NBI74_004112"
     CDS             64..1230
                     /locus_tag="NBI74_004112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HCD1934360.1"
                     /translation="MIEHYIRGFEEELREIRHQIHENPELGLQEFKTSALVAEKLRQW
                     GYEVEQGLATTGVVATLKVGDGEKSIGLRADMDALPIYENSGKPWASKHPGLMHACGH
                     DGHTTILLGAARYFAETRRFNGTLRLIFQPAEEMINGGEIMVKEGLFDRFPCDVIFGM
                     HNMPGLPVGKFFFQPGALMASMDQFHITVRGCGGHGAIPHKAIDPVLVAAHITTALQS
                     IVSRNVDPLEAAVITVGSIVAGEAANVIPDSAEMKISVRSLSRDTRQLLLTRIPALAQ
                     TQAASFGATAEVTHVNGTPVLVNDEEMARFAWQVACKTFGEDRAEFGIKPLMGSEDFS
                     FMLEAQPKGGFLLFGNGDVGEGSCMVHNPGYDFNDASLVPASSYWGALVEAWLQ"
     gene            1273..2589
                     /locus_tag="NBI74_004113"
     CDS             1273..2589
                     /locus_tag="NBI74_004113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCD1934361.1"
                     /translation="MTAIVNETVSVKKKPSGRRVIFASAFGNALEFFDFGVYNFFVVY
                     ISTLFFPPSADHNVALLLAFATFGVSFFMRPLGGIIVGAWADRFGRKPAMVFTIALMS
                     LGTLMIGIAPTYETAGYWGTATLVLARLIQGVAAGGEVGASMSLLVESAPANRRGFYS
                     SWSLATQGLATTFGGVVALGLSAWLPFATGSETVMAEWGWRVPFFIGVLLAPIGCWLR
                     LSLENDVPEPVRNKKAATSESAFSLLLQHKATIVNGVLLAIGSTVATYISLFYYGTWA
                     AKYLAMPQHYSHAAMLLAGVVTFVGALLVGMLCDSVGRKKLILISRVMVLLCSWPSFW
                     LLVNYPSPGMLLTVVFVMVSFTTLGGVPVMLLISELLPKRIRALGFALVYSIGVAIFG
                     GFAQYFATQSIVLLDSLTAPAWYLGGGTLLSMLALLYVKEPAKELQ"
     gene            2693..3343
                     /gene="fabR"
                     /locus_tag="NBI74_004114"
     CDS             2693..3343
                     /gene="fabR"
                     /locus_tag="NBI74_004114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor FabR"
                     /protein_id="HCD1934362.1"
                     /translation="MMGVRAQQKEKTRRSLVEAAFSQLSAERSFASLSLREVAREAGI
                     APTSFYRHFRDVDELGLTMVDESGLMLRQLMRQARQRIAKGGSVIRTSVSTFMEFIGN
                     NPNAFRLLLRERSGTSAAFRAAVAREIQHFIAELADYLELENHMPRAFTEAQAEAMVT
                     IVFSAGAEALDVGVEQRRQLEERLVLQLRMISKGAYYWYRREQEKTAIIPGNVKDE"
     gene            3343..3702
                     /locus_tag="NBI74_004115"
     CDS             3343..3702
                     /locus_tag="NBI74_004115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YijD family membrane protein"
                     /protein_id="HCD1934363.1"
                     /translation="MKQANQDRGTLLLALVAGLSINGTFAALFSSIVPFSVFPIISLV
                     LTVYCLHQRYLNRTMPVGLPGLAAACFILGVLLYSTVVRAEYPDIGSNFFPAVLSVIM
                     VFWIGAKMRNRKQDVAE"
     gene            complement(3742..4842)
                     /gene="trmA"
                     /locus_tag="NBI74_004116"
     CDS             complement(3742..4842)
                     /gene="trmA"
                     /locus_tag="NBI74_004116"
                     /EC_number="2.1.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (uridine(54)-C5)-methyltransferase TrmA"
                     /protein_id="HCD1934364.1"
                     /translation="MTPEHLPTEQYEAQLAEKVVRLQSMMAPFSDLVPEVFRSPVSHY
                     RMRAEFRIWHDGDDLYHIIFDQQTKSRIRVDSFPAASELINQLMTAMIAGVRNNPILR
                     HKLFQIDYLTTLSNQAVVSLLYHKKLDDEWCQQAEALRDALRAQNLNVHLIGRATKTK
                     IALDQDYIDERLPIAGKEMIYRQVENSFTQPNAAMNIQMLEWALDVTKGSKGDLLELY
                     CGNGNFSLALARNFDRVLATEIAKPSVAAAQYNIAANHIDNVQIIRMAAEEFTQAMNG
                     VREFNRLQGIDLKSYQCETIFVDPPRSGLDSETEKMVQAYPRILYISCNPETLCKNLE
                     TLSQTHKVERLALFDQFPYTHHMECGVLLTAK"
     regulatory      4956..5146
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            5211..7055
                     /gene="btuB"
                     /locus_tag="NBI74_004117"
     CDS             5211..7055
                     /gene="btuB"
                     /locus_tag="NBI74_004117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent vitamin B12 receptor BtuB"
                     /protein_id="HCD1934365.1"
                     /translation="MIKKASLLTACSVTAFSAWAQDTSPDTLVVTANRFEQPRSTVLA
                     PTTVVTRQDIDRWQSTSVNDVLRRLPGVDITQNGGSGQLSSIFIRGTNASHVLVLIDG
                     VRLNLAGVSGSADLSQFPIALVQRVEYIRGPRSAVYGSDAIGGVVNIITTRDHPGTEI
                     SAGWGSNSYQNYDVSTQQQLGDKTRVTLLGDYAHTHGYDVVAYGNTGTQAQPDNDGFL
                     SKTLYGALEHNFTDVWSGFVRGYGYDNRTNYDAYYSPGSPLVDTRKLYSQSWDAGLRY
                     NGELIKSQLITSYSHSKDYNYDPHYGRYDSSATLDEMKQYTVQWANNIIIGHGNIGAG
                     VDWQKQSTAPGTAYVEDGYDQRNTGIYLTGLQQVGDFTFEGAARSDDNSQFGRHGTWQ
                     TSAGWEFIEGYRFIASYGTSYKAPNLGQLYGFYGNPNLDPEKSKQWEGAFEGLTAGVN
                     WRISGYRNDVSDLIDYDDHTLKYYNEGKARIKGVEATANFDTGPLTHTVSYDYVDARN
                     AITDTPLLRRAKQQVKYQLDWQLYDFDWGITYQYLGSRYDKDYSSYPYQTVKMGGVSL
                     WDLAVAYPVTSHLTVRGKIANLFDKDYETVYGYQTAGREYTLSGSYTF"
     gene            7000..7857
                     /gene="murI"
                     /locus_tag="NBI74_004118"
     CDS             7000..7857
                     /gene="murI"
                     /locus_tag="NBI74_004118"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001312258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HCD1934366.1"
                     /translation="MATKLQDGNTPCLAATPSEPRPTVLVFDSGVGGLSVYDEIRHLL
                     PDLHYIYAFDNVAFPYGEKSEVFIVERVVEIVTAVQERYPLALAVVACNTASTVSLPA
                     LREKFDFPVVGVVPAIKPAARLTANGIVGLLATRGTVKRSYTHELIARFANECQIEML
                     GSAEMVELAEAKLHGEDVSLDALKRILRPWLRMKEPPDTVVLGCTHFPLLQEELLQVL
                     PEGTRLVDSGAAIARRTAWLLEHEAPDAKSADANIAFCMAMTPEAEQLLPVLQRYGFE
                     TLEKLAVLD"
BASE COUNT         1848 a         1909 c         2194 g         2130 t
ORIGIN      
        1 gtatttatac catcaataaa atccgttaat tagcctgttt ataagtgaat aaaggaatag
       61 ccgatgatcg aacactatat tcgtgggttt gaggaagaac ttcgcgagat ccgccatcag
      121 atccacgaaa acccggagct gggattacaa gaatttaaaa ccagtgcttt ggttgcggaa
      181 aagctgcgcc agtggggcta tgaggttgag caagggctgg cgacaaccgg cgtggtagcc
      241 acgttaaaag tgggcgacgg agagaaaagt attggcctgc gggcggatat ggatgcgttg
      301 ccgatttatg aaaatagtgg caagccctgg gccagtaaac atcctggttt aatgcacgcc
      361 tgcgggcatg acgggcatac caccatactg ctgggggccg cgcgttattt tgccgaaacc
      421 cgccgtttta acggcacgtt acgccttatc ttccagccag cggaagagat gattaacggc
      481 ggtgagatca tggttaaaga ggggcttttt gatcgtttcc cctgcgatgt cattttcggg
      541 atgcacaaca tgccgggttt gccggtcggc aagtttttct tccagcctgg ggcgctgatg
      601 gcgtcaatgg atcagttcca tattacggtt cgtggttgtg gcggacacgg tgcgatcccg
      661 cacaaagcca tcgatccagt gctggttgcc gcacatatca ccaccgcatt acaaagcatt
      721 gtgtcgcgca atgtcgatcc gctggaagcg gcggtcatta ccgtcggcag cattgttgcg
      781 ggtgaggcgg ctaacgtcat tcctgacagt gctgagatga aaatcagtgt ccgctctctt
      841 agccgcgata cccgacaact tcttctgacc cgtattcctg ctcttgcaca aacccaggct
      901 gccagctttg gcgctacggc tgaggttacg catgttaacg gtacaccggt attagtaaat
      961 gacgaggaga tggcgcgctt cgcctggcag gtggcgtgca aaacatttgg tgaagatcgg
     1021 gctgagtttg gcatcaaacc cctgatgggc agtgaagatt tctctttcat gctggaggcc
     1081 caacccaaag gcggtttcct gttattcggt aatggcgatg tcggggaagg ttcctgcatg
     1141 gtgcataacc ccggttacga ctttaacgat gcaagcctgg tcccggcaag cagctactgg
     1201 ggcgcactgg ttgaagcctg gctgcaataa ataaaacaac acacaacaac actttttggc
     1261 aaggaacatg gaatgacggc gatcgtaaat gaaaccgtga gtgtgaagaa aaagcccagc
     1321 ggcaggcggg tgatctttgc ctcggcgttt ggcaatgcgc tcgaattttt tgattttggc
     1381 gtttacaact tttttgtggt ctatatcagt acgcttttct tcccacccag tgccgatcat
     1441 aacgtcgcgc tgttactggc atttgcaacg tttggtgtga gcttttttat gcgtccgctc
     1501 ggtgggatta tcgttggtgc ctgggccgat cgcttcgggc gtaaaccggc gatggtgttc
     1561 actattgctc tgatgagcct cggtacgctg atgatcggca tcgcaccgac gtatgaaacg
     1621 gcgggctatt ggggaaccgc aacgctggtg ctggcgcgtt tgattcaggg cgttgcggca
     1681 ggtggtgaag tcggggcttc aatgtcgcta ctggtggagt cagctccggc aaatcgtcgt
     1741 ggcttttaca gcagttggtc actggcgact caaggcctgg caacaacttt tggcggtgtt
     1801 gtcgctttag gtttaagcgc atggctaccg ttcgccacag gttcagaaac cgtaatggca
     1861 gaatggggct ggcgtgtgcc gttttttatt ggtgtgttat tagcacctat cggttgctgg
     1921 ttgcgtctga gcctggaaaa tgacgtacct gagcctgtgc gcaataaaaa agctgctacc
     1981 agcgaaagcg cgttttctct gctcctgcaa cataaggcga ctatcgttaa tggcgtttta
     2041 ctcgctattg gtagcacggt cgcaacctat atctccctct tttattacgg cacctgggcg
     2101 gcgaagtatc tggcgatgcc tcaacattat tctcatgcag cgatgttact ggcgggcgtt
     2161 gttacgtttg tgggtgcgct gcttgtgggg atgttgtgcg attcggtcgg gcgtaaaaag
     2221 ctgattttaa tctcccgtgt gatggtgctt ctctgtagct ggccgtcatt ctggctgttg
     2281 gtgaattacc caagccctgg catgttgctg acggtggttt tcgtgatggt cagctttacc
     2341 acgcttggcg gtgtgccagt gatgttgttg atctctgaac tgttgccgaa acggattcgg
     2401 gcattgggtt ttgcgctggt ttatagcatt ggtgtggcaa tctttggcgg ttttgctcaa
     2461 tatttcgcta cgcaatccat cgtgttgctg gatagtctga cggctccggc gtggtatctg
     2521 ggtggaggca cgttgctgtc gatgctggcg ttgttgtacg taaaagaacc ggccaaagaa
     2581 ttgcagtaaa tatgttttat tgcgttaccg ttcattcaca acactggagc aatccagtat
     2641 gttcattctc tggtatagtg ccagcagtac ttttggcaag gattcagaca tcgtgatggg
     2701 cgtaagagcg caacaaaaag aaaaaacccg ccgttcgctg gtggaagccg catttagcca
     2761 attaagtgct gaacgcagct tcgccagcct gagtttgcgt gaagtggcac gagaagcagg
     2821 cattgctccc acctcttttt atcggcattt ccgcgacgta gacgaactgg gtctgaccat
     2881 ggttgatgag agcggtttaa tgctacgcca actcatgcgt caggcgcgtc agcgtatcgc
     2941 caaaggcggg agtgtgatcc gcacctcggt ctccacattt atggagttca tcggtaataa
     3001 tcctaacgcc ttccggttat tattgcggga acgctccggc acctccgctg cgtttcgtgc
     3061 cgccgttgcg cgtgaaattc agcacttcat tgcggaactt gcggactatc tggaactcga
     3121 aaaccatatg ccgcgtgcgt ttactgaagc gcaagccgaa gcaatggtga cgattgtctt
     3181 cagtgcgggg gccgaggcgt tggatgtcgg cgtcgagcaa cgtcggcaat tagaagagcg
     3241 actggtactg cagctgcgaa tgatttcgaa aggggcttat tactggtatc gccgtgaaca
     3301 agagaaaacc gcaattattc cgggaaatgt gaaggacgag taatgaaaca agcaaatcaa
     3361 gatagaggta cgctgctgct ggcgctagtg gctggcttat cgattaatgg tactttcgca
     3421 gcgctgttta gctccattgt accattttct gtattcccga ttatttccct ggtgttgaca
     3481 gtttactgcc tgcatcaacg ttatcttaat cgcactatgc cggtaggctt gccgggtctg
     3541 gcagctgctt gttttattct cggcgtactg ctgtacagca cggtagttcg tgcggaatat
     3601 ccggatatcg gttccaactt cttcccggca gtactgtccg tcattatggt gttctggatt
     3661 ggcgcgaaga tgcgtaaccg taagcaggac gttgctgagt aatcagcatc atgccggatg
     3721 caggcgcatc cgacatgggt tttacttcgc ggtcagtaat acgccgcact ccatatggtg
     3781 cgtgtagggg aactgatcaa atagagccag acgttcgacc ttgtgcgtct ggcttaatgt
     3841 ttccagattc ttgcataacg tttccggatt gcaggagata tacagaatac gtggatacgc
     3901 ctgcaccatt ttctcggttt cactgtccag accgctgcgc ggtgggtcga caaaaatggt
     3961 ttcgcactga taactcttta agtcgattcc ttgcaggcgg ttaaactcgc gcacaccatt
     4021 catcgcctga gtaaattctt ctgccgccat acgaataatt tgtacgttat caatatggtt
     4081 agcggcaatg ttgtattgcg cggcggcgac tgacggcttg gcgatttcgg tggctaatac
     4141 ccgatcaaaa ttgcgtgcca gcgctaatga aaagttaccg ttgccgcagt atagctccag
     4201 taaatcgcct tttgagcctt tggttacgtc cagcgcccat tccagcatct gaatattcat
     4261 cgccgcgttc ggttgggtaa agctgttttc tacctgacgg tagatcatct ctttccccgc
     4321 gatcggcaga cgttcgtcga tgtaatcctg atccagcgcg attttggttt tcgttgcccg
     4381 accaatcaga tgcacattca gattctgcgc gcgcagtgca tcgcgcaggg cttccgcctg
     4441 ctgacaccac tcatcatcca gctttttatg gtacagcaag gaaacaaccg cttgattgct
     4501 tagcgtagtg aggtaatcaa tctggaacaa cttgtggcgc agaatgggat tattacgcac
     4561 acccgcaatc atcgccgtca tcaactggtt gataagttca ctggcggcgg ggaagctatc
     4621 cacacggata cgacttttgg tttgttgatc gaaaatgatg tggtacaggt catcgccatc
     4681 gtgccagatg cggaactccg cacgcatccg ataatgactg accggcgagc gaaacacttc
     4741 cggaaccagg tcagaaaacg gtgccatcat actttgcaaa cgtaccactt tttcggctaa
     4801 ctgcgcttca tactgttctg ttggaaggtg ttcgggggtc atgatgtatc ctgaaaaatt
     4861 aaagtacgcg gcgattgtag ggattgctca tcagatgtcc agatcttgat gaattcctat
     4921 ttgtgagcta cgtctggaca gtaacttgtt acaacctgta gcatccactt gccggtcctg
     4981 tgagttaata gggaatccag tgcgaatctg gagctgacgc gcagcggtaa ggaaaggtgc
     5041 gatgattgcg ttatgcggac actgccatcc ggtgggaagt catcatctct tagtatctta
     5101 gatacccctc caagcccgaa gacctgccgg ccaacgtcgc atctggttct catcatcgcg
     5161 taatattgat gaaacctgcg gcatccttct tctattgtgg atgctttaca atgattaaaa
     5221 aagcttcgct gctgacggcg tgttccgtca cggcattttc cgcttgggca caggatacca
     5281 gcccggatac tctcgtcgtt actgctaacc gttttgaaca accgcgcagc actgtgcttg
     5341 cgccaactac tgttgtgacg cgtcaggata tcgaccgctg gcagtcgacc tcggtcaatg
     5401 atgtgctgcg ccgtcttccg ggcgtggata tcacccaaaa cggcggttca ggtcagctct
     5461 catctatttt tattcgcggt acaaatgcca gtcatgtgtt ggtgttaatt gatggcgtcc
     5521 gcctgaattt ggcgggggtg agtggttctg ctgatcttag tcagttccct attgcgcttg
     5581 tccagcgtgt tgaatatatc cgtgggccgc gctccgctgt ttatggttca gatgcaatag
     5641 gcggggtcgt gaatatcatc acgacgcgcg atcaccccgg aacggaaatt tcagcagggt
     5701 ggggaagcaa tagttatcag aactatgatg tctctacaca gcaacaactg ggggataaga
     5761 cacgggtaac gctgttgggc gattatgccc atactcatgg ttatgatgtt gtcgcctatg
     5821 gtaataccgg aacgcaagcg cagccagata acgatggttt tttaagtaaa acgctttatg
     5881 gcgcgctgga gcataatttt actgatgtct ggagcggctt tgtgcgcggc tatggctatg
     5941 ataatcgtac caattatgac gcatattatt ctcccggctc gccactggtc gatacccgta
     6001 aactctatag ccaaagttgg gatgccggac tgcgctataa cggtgaactg attaaatcac
     6061 aactgattac cagctacagc catagcaaag attacaacta cgatccgcat tatggtcgtt
     6121 atgattcatc cgcgacgctc gatgagatga aacagtacac cgtccagtgg gcaaacaaca
     6181 tcatcattgg ccacggtaat attggtgcgg gtgttgactg gcagaagcag agcacggcac
     6241 cgggcacagc ttatgttgag gatggatatg atcaacgtaa taccggcatc tatctgaccg
     6301 ggctgcaaca agttggcgat tttacctttg aaggcgcagc acgtagcgac gataactcac
     6361 agtttggtcg tcatggcacc tggcaaacca gcgccggttg ggaatttatc gaaggttatc
     6421 gctttattgc ttcctacggg acatcttata aggcaccaaa tcttggacaa ctgtatggtt
     6481 tctacggaaa tccgaatctc gaccctgaga aaagcaaaca gtgggaaggc gcgtttgaag
     6541 gcttaactgc tggggtgaac tggcgtattt ccggatatcg taacgatgtc agtgacttga
     6601 tcgattatga tgatcacacc ctgaaatatt acaacgaagg gaaagcgcgg attaagggtg
     6661 tcgaggcgac cgccaatttt gataccggac cactgacgca tactgtgagt tatgattatg
     6721 ttgatgcgcg caatgcgatt actgacacgc cgttgttacg ccgtgctaaa cagcaggtga
     6781 aataccagct cgactggcag ttgtatgact tcgactgggg gattacttat cagtacttag
     6841 gttctcgtta tgataaggat tactcctctt atccgtatca aacagtgaaa atgggcgggg
     6901 tgagcttgtg ggatcttgct gttgcatatc cggtcacctc tcacctgaca gttcgtggta
     6961 aaatagccaa cctgttcgac aaagattacg agacagtcta tggctaccaa actgcaggac
     7021 gggaatacac cttgtctggc agctacacct tctgaaccac gtcccaccgt gctggtgttt
     7081 gactccggcg tcggtgggtt gtcggtctat gacgagatcc ggcatctctt gccggatctc
     7141 cattacattt atgctttcga taacgtcgct ttcccgtatg gcgaaaaaag cgaagtgttt
     7201 attgttgagc gagtggtgga aattgtcacc gcagtgcaag aacgttatcc ccttgcgctg
     7261 gctgtggtcg cttgcaacac cgccagtacc gtttcacttc ctgcattacg cgaaaagttc
     7321 gacttcccgg ttgttggtgt cgtgccggcg attaaacctg ctgcacgtct gacggcaaat
     7381 ggcattgtcg gattactggc aacccgcgga actgttaaac gttcttatac tcatgagctg
     7441 atcgcgcgtt tcgctaatga atgccagata gaaatgctgg gctcggcaga gatggttgag
     7501 ttggctgaag cgaagctgca tggcgaagat gtttctctgg atgcgctaaa acgtatccta
     7561 cgcccgtggt taagaatgaa agagccgcca gatacagttg tattgggttg cacccatttc
     7621 cctctactac aagaagaact attacaagtg ctgccagagg gaacccggct ggtggattct
     7681 ggcgcagcga ttgctcgccg aacggcctgg ttgttagagc atgaagcccc ggatgcaaaa
     7741 tctgccgatg cgaatattgc attttgtatg gcaatgacgc cagaagctga acaattattg
     7801 cccgttttac agcgttacgg cttcgaaacg ctcgaaaaac tggcagtttt agattgattt
     7861 ggttgaatgt tgcgcggtca gaaaattatt ttaaatttcc tcttgtcagg ccggaataac
     7921 tccctataat gcgccaccac tgacacggaa caacggcaaa cacgccgccg ggtcagcggg
     7981 gttctcctga gaatctcaac agaggaaagc aaaaaaatgc ttgactctgt agcgggaaag
     8041 cgtattatgt ataccccgcg ccgctgagaa aagcgaagcg g
//
LOCUS       DAHHTG010000174         7865 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.177, whole genome shotgun sequence.
ACCESSION   DAHHTG010000174 DAHHTG010000000
VERSION     DAHHTG010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7865)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7865)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7865
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.177"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            121..210
                     /gene="kdpF"
                     /locus_tag="NBI74_004119"
     CDS             121..210
                     /gene="kdpF"
                     /locus_tag="NBI74_004119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HCD1934367.1"
                     /translation="MSAGVITGVLLVFLLLGYLVYALINAEAF"
     gene            210..1883
                     /gene="kdpA"
                     /locus_tag="NBI74_004120"
     CDS             210..1883
                     /gene="kdpA"
                     /locus_tag="NBI74_004120"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HCD1934368.1"
                     /translation="MAAQGFLLIATFLLVLMVLARPLGSGLARLINDIPLPGTTGVER
                     VLFSALGVSDREMNWKQYLSAILGLNILGLAVLFFMLLGQHYLPLNPQQLPGLSWDLA
                     LNTAVSFVTNTNWQSYSGETTLSYFSQMAGLTVQNFLSAASGIAVIFALIRAFTRQSM
                     STLGNAWVDLLRITLWVLVPVALLIALFFIQQGALQNFLPYQAVNTVEGAQQLLPMGP
                     VASQEAIKMLGTNGGGFFNANSSHPFENPTALTNFVQMLAIFLIPTALCFAFGEVAGD
                     RRQGRMLLWAMSVIFVICVGVVMWAEVQGNPHLLALGADSSINMEGKESRFGVLVSSL
                     FAVVTTAASCGAVIAMHDSFTALGGMVPMWLMQIGEVVFGGVGSGLYGMMLFVLLAVF
                     IAGLMIGRTPEYLGKKIDVREMKLTALAILVTPTLVLMGAALAMMTDAGRSAMLNPGP
                     HGFSEVLYAVSSAANNNGSAFAGLSANSPFWNCLLALCMFVGRFGVIIPVMAIAGSLV
                     SKKSQPASSGTLPTHGPLFVGLLIGTVLLVGALTFIPALALGPVAEYLS"
     gene            1906..3954
                     /gene="kdpB"
                     /locus_tag="NBI74_004121"
     CDS             1906..3954
                     /gene="kdpB"
                     /locus_tag="NBI74_004121"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
                     /protein_id="HCD1934369.1"
                     /translation="MSRKQLALFEPTLVVQALKEAVKKLNPQAQWRNPVMFIVWIGSL
                     LTTCISIAMASGVMPGNALFSAAISGWLWVTVLFANFAEALAEGRSKAQANSLKGVKK
                     TAFARKLREPKYGAVADKVPADQLRKGDIVLVEAGDIIPCDGEVIEGGASVDESAITG
                     ESAPVIRESGGDFASVTGGTRILSDWLVIECSVNPGETFLDRMIAMVEGAQRRKTPNE
                     IALTILLIALTIVFLLATATLWPFSAWGGNAVSVTVLVALLVCLIPTTIGGLLSAIGV
                     AGMSRMLGANVIATSGRAVEAAGDVDVLLLDKTGTITLGNRQASEFIPAQGVDEKTLA
                     DAAQLASLADETPEGRSIVILAKQRFNLRERDVQSLHATFVPFTAQSRMSGINIDNRM
                     IRKGSVDAIRRHVEANGGHFPADVDQKVDQVARQGATPLVVVEGSRVLGVIALKDIVK
                     GGIKERFAQLRKMGIKTVMITGDNRLTAAAIAAEAGVDDFLAEATPEAKLALIRQYQA
                     EGRLVAMTGDGTNDAPALAQADVAVAMNSGTQAAKEAGNMVDLDSNPTKLIEVVHIGK
                     QMLMTRGSLTTFSIANDVAKYFAIIPAAFAATYPQLNALNIMRLHSPDSAILSAVIFN
                     ALIIVFLIPLALKGVSYKPLTASAMLRRNLWIYGLGGLLVPFIGIKVIDLLLTVCGLV
                     "
     gene            3963..4535
                     /gene="kdpC"
                     /locus_tag="NBI74_004122"
     CDS             3963..4535
                     /gene="kdpC"
                     /locus_tag="NBI74_004122"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit C"
                     /protein_id="HCD1934370.1"
                     /translation="MSGLRPALSTFLFLLLITGGVYPLLTTALGQWWFPWQANGSLIR
                     EGDTVRGSALIGQNFTGNGYFHGRPSATAEMPYNPQASGGSNLAVSNPVLDKQIAARV
                     AALRAANPDASTSVPVELVTASASGLDNNITPQAAAWQIPRVAKARNLSVEQLTQLIA
                     KYSQQPLVNYIGQPVVNVVELNLALDKLDE"
     gene            4528..7212
                     /gene="kdpD"
                     /locus_tag="NBI74_004123"
     CDS             4528..7212
                     /gene="kdpD"
                     /locus_tag="NBI74_004123"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     KdpD"
                     /protein_id="HCD1934371.1"
                     /translation="MNNEPLRPDPDRLLEQTAAPHRGKLKVFFGACAGVGKTWAMLAE
                     AQRLRAQGLDIVVGVVETHGRKDTAAMLEGLAVLPLKRQAYRGRHISEFDLDAALARR
                     PALILMDELAHSNAPGSRHPKRWQDIEELLEAGIDVFTTVNVQHLESLNDVVSGVTGI
                     QVRETVPDPFFDAADDVVLVDLPPDDLRQRLKEGKVYIAGQAERAIEHFFRKGNLIAL
                     RELALRRTADRVDEQMRAWRGHPGEEKVWHTRDAILLCIGHNTGSEKLVRAAARLASR
                     LGSVWHAVYVETPALHRLPEKKRRAILSALRLAQELGAETATLSDPAEEKAVVRYARE
                     HNLGKIILGRPASRRWWRRETFADRLARIAPDLDQVLVALDEPPARTINNAPDSRSFK
                     DKWRVQIQGCVVAAALCAVITLIAMQWLMAFDAANLVMLYLLGVVVVALFYGRWPSVV
                     ATVINVVSFDLFFIAPRGTLAVSDVQYLLTFAVMLTVGLVIGNLTAGVRYQARVARYR
                     EQRTRHLYEMSKALAVGRSSQDIAATSEQFIASTFHARSQVLLPDDNGKLQPLTHPQG
                     MTPWDDAIAQWSFDKGLPAGAGTDTLPGVPYQILPLKSGEKTYGLVVVEPGNLRQLMI
                     PEQQRLLETFTLLVANALERLTLTASEEQARMASEREQIRNALLAALSHDLRTPLTVL
                     FGQAEILTLDLASEGSPHARQASEIRQHVLNTTRLVNNLLDMARIQSGGFNLKKEWLT
                     LEEVVGSALQMLEPGLSSPINLSLPEPLTLIHVDGPLFERVLINLLENAVKYAGAQAE
                     IGINAHIEGENLQLDVWDNGPGLPPGQEQTIFDKFARGNKESAVPGVGLGLAICRAIV
                     DVHGGTITAFNRPEGGACFRVTLPQQTAPELEEFHEDM"
     gene            7209..>7865
                     /gene="kdpE"
                     /locus_tag="NBI74_004124"
     CDS             7209..>7865
                     /gene="kdpE"
                     /locus_tag="NBI74_004124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator KdpE"
                     /protein_id="HCD1934372.1"
                     /translation="MTNVLIVEDEQAIRRFLRTALEGDGMRVFEAETLQRGLLEAATR
                     KPDLIILDLGLPDGDGIEFIRDLRQWSAVPVIVLSARSEESDKIAALDAGADDYLSKP
                     FGIGELQARLRVALRRHSATAAPDPLVKFSDVTVDLAARVIHRGEEEVHLTPIEFRLL
                     AVLLNNAGKVLTQRQLLNQVWGPNAVEHSHYLRIYMGHLRQKLEQDPARPRHFITETG
                     I"
BASE COUNT         1614 a         2074 c         2332 g         1845 t
ORIGIN      
        1 taaatcattg cttttgccat ttttatactt tttttacacc acgcccgcag atttttgcga
       61 aatctttgca gccagaactc tacccttccg gtatcacttt taggccactg gaggtgcact
      121 gtgagtgcag gcgtgataac cggcgtgttg ctggtgtttt tattactggg ttatctggtt
      181 tatgccctga tcaatgcgga ggcattctga tggctgcgca agggttctta ctgattgcca
      241 cgtttttact ggtattaatg gtgctggcgc gtcccttagg tagcgggctg gcgcggctga
      301 ttaatgatat tcctcttcct ggtacgacgg gcgttgaacg cgtactcttt agcgcgcttg
      361 gcgtctctga ccgcgagatg aactggaagc agtatctctc agccattctt ggcctgaata
      421 tactggggct ggcggtgctg ttttttatgt tgctcggtca gcactatctg ccgcttaatc
      481 cacagcagtt gccagggctg tcgtgggatc tggcgctcaa taccgccgtc agctttgtca
      541 ccaataccaa ctggcaatct tatagcggtg aaaccacgtt gagctatttc agccagatgg
      601 cgggcttaac ggtacaaaac tttctttctg ccgccagcgg gattgcggtg atttttgccc
      661 tcattcgtgc atttacccgc cagagcatga gcacactcgg gaatgcctgg gtcgatctgc
      721 tacgcatcac gttatgggtg ctagtccctg tggcgttgtt gatcgcactg ttttttattc
      781 aacaaggtgc gctgcaaaac tttctgccct atcaggcggt gaataccgtt gaaggagcac
      841 aacagctgtt acccatgggg ccagtagctt ctcaggaagc gatcaaaatg ctcggaacta
      901 acggcggtgg cttttttaat gccaactcgt cgcatccgtt tgaaaaccca accgcactga
      961 ccaacttcgt gcaaatgctg gcgattttct tgatcccaac agcgctgtgc tttgcctttg
     1021 gtgaagtggc gggcgatcgt cgccaggggc gcatgttgct gtgggcgatg tcggtgattt
     1081 ttgtcatctg cgtaggcgta gtgatgtggg cagaagttca gggtaatcct catctgctgg
     1141 cgctcggcgc ggacagcagc atcaatatgg aaggtaaaga gagccgtttc ggcgtgctgg
     1201 tcagtagcct gtttgcggtc gtgactacgg cggcttcctg cggcgcggtg attgcgatgc
     1261 atgattcgtt taccgctctt ggtggcatgg tgccgatgtg gttgatgcaa attggtgaag
     1321 tggtattcgg cggcgtcggt tccggccttt acggcatgat gctgtttgtc ctgctggcgg
     1381 tgtttattgc cgggctgatg attggtcgta caccggaata tctgggtaaa aaaatcgacg
     1441 tacgcgagat gaaactgact gcactggcga ttctggtcac cccgacgctg gtgctgatgg
     1501 gcgcggcgct ggcaatgatg accgacgccg gacgtagcgc catgctcaac cctggcccgc
     1561 atggttttag cgaagtgctg tacgccgtgt catccgccgc taacaacaac ggcagcgcct
     1621 ttgccggatt aagcgccaac tctccattct ggaactgttt actggcgttg tgcatgtttg
     1681 tcgggcgctt cggggtgatt atcccggtga tggcaattgc cggttcgctg gtgagtaaaa
     1741 agagccaacc cgccagctcc ggcacattgc caacgcacgg cccgctgttt gttggcctgt
     1801 taatcggcac cgtgttgctg gttggcgcac tgacctttat ccctgccctg gcgcttggtc
     1861 cggtggcgga atatctctcc tgatgatatt gagtgagcac tgaatatgag tcgtaaacaa
     1921 ctggcgctat tcgaaccaac acttgtcgtt caggcgctga aagaagcggt gaaaaaatta
     1981 aacccgcagg cgcaatggcg caatccggtg atgtttatcg tctggatcgg cagtctgctg
     2041 accacctgta ttagcatcgc gatggcaagc ggtgtgatgc ctggtaatgc gctgtttagc
     2101 gcggcaatta gcggctggct gtgggtcacc gtactgttcg ctaatttcgc cgaggcgttg
     2161 gcagaaggcc gcagtaaagc gcaggccaac agtctgaaag gggtgaaaaa aaccgctttt
     2221 gctcgcaagc tgcgcgaacc gaaatatggc gctgtggcag acaaagttcc tgccgatcaa
     2281 ctgcgtaaag gcgatatcgt actggtagaa gctggcgata ttatcccctg cgatggtgaa
     2341 gtcattgaag gcggcgcatc ggtcgatgaa agcgccatca ccggggaatc ggctccggtg
     2401 atccgtgaat ccggcggtga ttttgcctcc gtcactggcg gcacgcgtat tctttctgac
     2461 tggctggtga ttgagtgcag cgttaacccc ggcgagacat ttctggatcg gatgatcgcg
     2521 atggtggaag gtgcgcagcg acgtaaaacg ccgaacgaga ttgccctgac cattctcctg
     2581 attgccctga ctatcgtctt tttactggca accgccacgc tgtggccgtt ttccgcgtgg
     2641 ggcggtaatg cagtcagcgt aacggtactg gtggcactgc tggtctgtct gatcccaacc
     2701 actattggcg gcctgttgtc agcgattggc gtcgccggga tgagtcggat gctgggcgcg
     2761 aatgttattg ccaccagcgg gcgtgcagtt gaagcggcgg gcgacgtgga cgtcctgctg
     2821 ctggataaaa ccggcaccat cacactcggt aaccgtcagg cgtcggagtt tatccctgcg
     2881 cagggcgtgg atgaaaaaac gctggctgac gccgcacaac tggcttcgct ggctgatgaa
     2941 acgccggaag gccgcagtat tgtgatcctc gccaagcagc gttttaacct gcgcgagcgc
     3001 gatgtgcagt cgctccatgc cacctttgta ccgtttactg cgcaaagccg gatgagcggg
     3061 atcaacatcg acaaccgcat gatccgtaaa ggttctgtcg atgccattcg ccgccatgtt
     3121 gaggctaacg gcggtcactt ccctgccgat gttgatcaaa aagtcgatca agttgcgcgt
     3181 cagggagcca cgccgctggt ggtggtggaa ggttctcgtg tgctgggcgt tattgcgctg
     3241 aaagatatcg tcaaaggcgg tattaaagag cgcttcgccc agctgcgtaa aatgggcatt
     3301 aaaacggtga tgattaccgg cgataaccgt ctgactgccg ccgcgattgc cgcggaagcg
     3361 ggcgtcgatg attttctcgc cgaagcaaca ccggaggcca agctggcgtt gattcgtcag
     3421 tatcaggcag aaggccgttt agtggcgatg accggcgatg gcaccaacga tgctccggcg
     3481 ctggcgcagg cggatgtcgc ggtggcgatg aactccggca cccaggcggc gaaagaagca
     3541 ggcaatatgg tggatctcga ctccaacccg accaagttga tcgaggtggt gcacattggc
     3601 aaacagatgc tgatgacccg tggctcgttg accaccttca gcattgccaa cgatgtggcg
     3661 aaatacttcg ccattattcc agcggcgttc gcggcaacgt atccgcagtt aaatgcgctg
     3721 aacatcatgc gcctgcattc gcccgactcc gccattctca gtgcggtcat tttcaacgcc
     3781 ttgattatcg tctttttgat tcccctggcg ttaaaaggcg tgagttataa accgcttacc
     3841 gcttctgcca tgttgcgccg taacttatgg atttacggtc tgggtgggct gctggtgccg
     3901 tttattggta tcaaagtcat tgatttactg ctaaccgttt gcggtctggt gtgaggttta
     3961 ccatgagtgg attacgtccg gcattatcga catttctctt tctgttattg attactggcg
     4021 gcgtttaccc gctgctgacc accgcactgg ggcaatggtg gtttccctgg caggccaacg
     4081 gttcattgat tcgtgaaggt gatacggtgc gcggttcggc attaatcggg caaaatttta
     4141 ccggcaacgg ctattttcat ggtcgcccgt cggcaacagc agaaatgccc tataatccac
     4201 aggcttctgg cgggagcaat ctggcagtca gtaacccggt gctggataaa cagatagccg
     4261 cacgcgttgc cgcattacgg gccgctaacc cggacgccag cacgagcgtt ccggttgaac
     4321 tggtgacagc gtcagcaagc gggctggaca ataatattac cccgcaagcg gcggcctggc
     4381 agatcccacg cgtggcgaaa gcgcgtaatc tcagcgttga acagctcacg caactgatcg
     4441 caaaatacag ccaacaaccg ctggtgaact atatcggcca gccggtcgtc aacgttgtag
     4501 aactcaatct ggcgctggat aagcttgatg aataacgaac ccttacgtcc cgaccccgat
     4561 cgtctgctgg aacaaactgc cgcgccgcat cgggggaagc tgaaagtttt cttcggtgcc
     4621 tgcgcaggcg tcggaaagac ctgggcgatg ctggcagaag cccagcgact gcgggcgcaa
     4681 gggctggata ttgtggttgg cgtggtagaa acccacgggc ggaaagatac cgccgcaatg
     4741 ctggaaggtc tggctgttct gccgctgaaa cgccaggcgt accgtgggcg gcatatcagc
     4801 gagtttgatc tcgatgccgc cctcgcccgc cgtccggcac tgatcttaat ggacgaactg
     4861 gcgcacagta atgcgccagg ttctcgtcat cctaaacgct ggcaggacat cgaagagctg
     4921 ctggaagcag gcattgatgt tttcactacc gtcaacgttc agcatctgga aagcctgaac
     4981 gatgtggtca gtggcgtaac cggaattcag gttcgggaaa ccgtgcccga tccttttttc
     5041 gatgccgccg acgacgtagt actggtggac ttgcccccgg acgatctgcg ccaacgactg
     5101 aaagaaggca aagtctatat tgccgggcag gcagaacgcg ccattgagca ttttttccgc
     5161 aaaggcaatc tgatcgccct gcgcgagctg gcgctacgcc gtactgccga tcgcgtagat
     5221 gagcaaatgc gcgcgtggcg ggggcatcct ggcgaagaga aagtctggca cacgcgcgac
     5281 gcgatccttt tatgcatcgg gcataacacc ggcagtgaga aactggtccg cgcggcggcg
     5341 cggctggcgt cgcgcctggg cagtgtctgg cacgcggtgt atgttgaaac ccctgccctg
     5401 catcgcttac cggagaaaaa acgtcgggca attctcagcg ccttacgtct ggcgcaggag
     5461 ttgggcgcgg agacggcaac actttctgat ccagcggaag aaaaagcagt agtgcgttat
     5521 gcccgtgaac ataatctcgg caagattatt ctcggtcgcc cggcctcgcg ccgctggtgg
     5581 cgtcgggaaa cgtttgctga ccggctggcg cgcatcgccc ccgatctcga tcaggtactg
     5641 gtcgcgcttg atgaaccacc cgcccgcacg attaataacg cgccggatag ccgctctttt
     5701 aaagacaagt ggcgggtaca gattcaggga tgcgtggttg ccgccgcgtt atgcgccgtt
     5761 atcaccttaa ttgccatgca gtggctgatg gcgtttgatg ccgccaacct ggtgatgctg
     5821 tatctgcttg gcgtggttgt ggtggcacta ttttacggac gctggccttc ggtggttgcg
     5881 accgtcatta atgtggtgag tttcgatctc ttttttatcg ccccacgcgg cacgctcgcc
     5941 gtctctgatg tgcaatatct gctgaccttc gcggtgatgt taaccgtcgg gctggtgatc
     6001 gggaacctta ctgctggcgt gcgttatcag gcgcgggtag cccgttaccg cgaacaacgc
     6061 acacggcact tatatgaaat gtccaaagct ctggctgtgg gccgtagttc gcaggatatc
     6121 gccgccacca gcgaacagtt tattgcctcc acgtttcatg cccgtagcca ggtgttgctg
     6181 cctgatgaca acggtaaatt gcagccttta acacatccgc aaggaatgac gccgtgggac
     6241 gatgccatcg cgcagtggag ttttgataaa ggcctgcctg cgggcgcggg caccgacacg
     6301 ttacccggtg taccgtacca gattttgccg ctaaaaagcg gcgagaaaac ctacggactg
     6361 gtggtggtgg aaccggggaa tctgcgccag ttgatgatcc cggaacagca gcgcctgctg
     6421 gagacgttta cgctgttagt cgccaatgcc cttgagcgac tgacactgac cgccagcgaa
     6481 gaacaggcgc ggatggcaag cgaacgtgaa cagatccgca acgccctgct ggcggcgctt
     6541 tcgcatgatt tacgcacgcc gcttacggtg ctgttcggtc aggcagaaat cttaacgctc
     6601 gatctggcaa gcgaaggatc gccccacgcc cgccaggcca gcgagattcg tcagcatgtg
     6661 ctgaacacca cccgactggt gaataatcta ctggatatgg cgcgaattca gtccggcggc
     6721 tttaatttga agaaagagtg gttaacgctg gaagaagtgg tcggcagcgc gctgcaaatg
     6781 ctggaaccgg gtttatcgtc gcccatcaat ctttctctgc cagagccgct gaccttaatc
     6841 cacgttgacg ggccactctt tgaacgggtg ctgattaacc tgctggagaa cgcggtgaaa
     6901 tatgcggggg cgcaggccga aattggtatc aatgcccaca tcgagggcga aaatctacaa
     6961 ctggatgtct gggataacgg ccccggtctt ccaccaggcc aggagcagac gatatttgat
     7021 aagtttgctc gcgggaataa agagtcggcg gtaccgggtg tagggcttgg gttggcaatt
     7081 tgtcgggcga tagtggatgt acacggcggc accattaccg cattcaaccg accggaaggt
     7141 ggtgcctgtt ttcgtgttac acttccccag caaacagccc ctgaacttga agaatttcat
     7201 gaggatatgt gacaaacgtt ctgattgttg aagatgaaca ggctattcgt cgctttctgc
     7261 gcacggcgct ggagggcgat gggatgcgcg tctttgaggc cgaaacgctg caacgcggct
     7321 tgctggaagc ggctacccgt aagccagatt tgattattct cgatctcggc ctgcccgatg
     7381 gtgatgggat tgagtttatc cgcgacctgc gccagtggag cgcggtgccg gtgattgtgc
     7441 tttccgcacg tagcgaagag agcgacaaaa tcgccgcgct ggatgccgga gcggatgatt
     7501 atctgagtaa gccgtttggc attggcgaat tgcaggcccg tctgcgcgtc gctttacgtc
     7561 gccactccgc caccgccgcg cccgatccgc tggtgaaatt ttccgatgtt accgtcgatt
     7621 tagccgcccg cgttatccat cggggcgagg aagaggtgca tctcaccccg attgagttcc
     7681 gcctgctggc agtgttgctc aacaacgccg gaaaagtgct cacccagcgc cagttactca
     7741 atcaggtgtg gggaccaaac gcggtcgaac acagccacta tttgcgtatt tatatggggc
     7801 atctgcgaca aaaactggag caagatcccg cccgcccccg ccattttatt actgaaaccg
     7861 gtatt
//
LOCUS       DAHHTG010000175         7858 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.178, whole genome shotgun sequence.
ACCESSION   DAHHTG010000175 DAHHTG010000000
VERSION     DAHHTG010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7858)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7858)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7858
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.178"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            complement(<1..433)
                     /locus_tag="NBI74_004125"
     CDS             complement(<1..433)
                     /locus_tag="NBI74_004125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001576399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1934373.1"
                     /translation="MASSDFSEELTTTAIWLRDKGVDIRCVRLTPYRFNDDVLINAEQ
                     IIPVPELEEYQVKFREKRDEQLISSQKKEKDYTWYICKDKKLNKRKLAIELLRDCIKQ
                     FNPASYNDLINGLSEDFKKRTVILIDQIPEKQKSRYHINEDA"
     gene            complement(403..741)
                     /locus_tag="NBI74_004126"
     CDS             complement(403..741)
                     /locus_tag="NBI74_004126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001375319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1934374.1"
                     /translation="MISDWEDSHRRIDLLAIDKQANIVVIELKRDETGTHMELQALRY
                     AAMIYTMSFAKACEYFQTYLKKQNRDADAKEKMRKKKYLNLLSWMKLSLLILVKISES
                     FWLHRILARN"
     gene            1156..1758
                     /locus_tag="NBI74_004127"
     CDS             1156..1758
                     /locus_tag="NBI74_004127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1819 family protein"
                     /protein_id="HCD1934375.1"
                     /translation="MKNDKAWIGDLLGGPLMSRESRVIAELLLTDPDEQTWQEQIVGH
                     NILQASSPNTAKRYAATIRLRLNTLDKSAWTLIAEGSERERQQLLFVALMLHSPVVKD
                     FLAEVVNDLRRQFKEKLPGNSWNEFVNSQVRLHPVLASYSDSSIAKMGNNLVKALAEA
                     GYVDTPRRRNLQAVYLLPETQAVLQRLGQQDLISILEGKR"
     gene            1755..2357
                     /locus_tag="NBI74_004128"
     CDS             1755..2357
                     /locus_tag="NBI74_004128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1788 domain-containing protein"
                     /protein_id="HCD1934376.1"
                     /translation="MIDPVLEYRLSQIQSRINEDRFLKNNGSGNEIGFWIFDYPAQCE
                     LQVREHLKYLLRHLEKDHKFACLNVFQIIIDMLNERGLFERVCQQEVKVGTETLKKQL
                     AGPLNQKKIADFIAKKVDLAAQDFVILTGMGNAWPLVRGHELMSALQDVMGFTPLLMF
                     YPGTYSGYNLSPLTDTGSQNYYRAFRLVPDTGPAATLNPQ"
     gene            2369..6010
                     /gene="brxC"
                     /locus_tag="NBI74_004129"
     CDS             2369..6010
                     /gene="brxC"
                     /locus_tag="NBI74_004129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BREX system P-loop protein BrxC"
                     /protein_id="HCD1934377.1"
                     /translation="MNIEQIFEKPLKRNINGVVKAEQTDDASAYIELDEYVITRELEN
                     HLRHFFESYVPATGPERIRMENKIGVWVSGFFGSGKSHFIKILSYLLSNRKVTHNGTE
                     RNAYSFFEDKIKDALFLADINKAVHYPTEVILFNIDSRANVDDKEDAILKVFLKVFNE
                     RIGYCADFPHIAHLERELDKRGQYETFKAAFADINGSRWEDERDAYYFISDDMAQALS
                     QATQQSLEASRQWVEQLDKNFPLDINNFCQWVKEWLDDNGKNILFMVDEVGQFIGKNT
                     QMMLKLQTITENLGVICGGRAWVIVTSQADINAAIGGMSSRDGQDFSKIQGRFSTRLQ
                     LSSSNTSEVIQKRLLVKTDEAKAALAKVWQEKGDILRNQLAFDPTTTTALRPFTSEEE
                     FVDNYPFVPWHYQILQKVFESIRTKGAAGKQLAMGERSQLEAFQTAAQQISAQGLDSL
                     VPFWRFYAAIESFLEPAVSRTITQACQNGILDEFDGNLLKTLFLIRYVETLKSTLDNL
                     VTLSIDRIDADKVELRRRVEKSLNTLERLMLIARVEDKYVFLTNEEKEIENEIRNVDV
                     DFSAINKKLASIIFDDILKSRKYRYPANKQDFDISRFLNGHPLDGAVLNDLVVKILTP
                     KDPTYTFYNSDATCRPYTSEGDGCILIRLPEEGRTWSDIDLVVQTEKFLKDNAGQRPE
                     QATLLSEKARENSNREKLLRVQLESLLAEADVWAIGERLPKKSSTPSNIVDEACRYVI
                     ENTFGKLKMLRPFNGDISREIHALLTVENDTELDLGNLEESNPDAMREVETWISMNIE
                     YNKPVYLRDILNHFARRPYGWPEDEVKLLVARLACKGKFSFSQQNNNVERKQAWELFN
                     NSRRHSELRLHKVRRHDEAQVRKAAQTMADIAQQPFNEREEPALVEHIRQVFEEWKQE
                     LNVFRAKAEGGNNPGKNEIESGLRLLNAILNEKEDFALIEKVSSLKDELLDFSEDRED
                     LVDFYRKQFATWQKLGAALNGSFKSNRSALEKDAAAVKALGELESIWQMPEPYKHLNR
                     ITPLIEQVQNVNHQLVEQHRQHALERIDARIEESRQRLLEAHATSELQNSVLLPMQKA
                     RKRAEVSQSIPEILAEQQETKALQMDADKKINLWIDELRKKQEAQLRAANEAKRAADS
                     EQTYVVVEKTVIQPVPKKTHLVNVASEMRNATGGEVLETTEQVEKALDTLRTTLLAVI
                     KAGDRIRLQ"
     gene            6056..>7858
                     /gene="pglX"
                     /locus_tag="NBI74_004130"
     CDS             6056..>7858
                     /gene="pglX"
                     /locus_tag="NBI74_004130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001466205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BREX-1 system adenine-specific
                     DNA-methyltransferase PglX"
                     /protein_id="HCD1934378.1"
                     /translation="MNTNNIKKYAPQARNDFRDAVVQKLTTLGIAADKKGNLQIAEAE
                     TIGETVRYGQFDYPLSTLPRRERLVKRAREQGFEVLVEHCAYTWFNRLCAIRYMELHG
                     YLEHGFRMLSHPETPTAFEVLDHVPEVAEALLPENKAQLVEMKLSGNQDEALYRELLL
                     GQCHALHHAMPFLFEAVDDEAELLLPDNLTRTDSILRGLVDDIPEEDWEQVEVIGWLY
                     QFYISEKKDAVIGKVVKSEDIPAATQLFTPNWIVQYLVQNSVGRQWLQTYPDSPLKDK
                     MEYYIEPAEQTPEVQAQLAAITPASIEPESIKVLDPACGSGHILIEAYNVLKNIYEER
                     GYRGRDIPQLILENNIFGLDIDDRAAQLSGFALLMMASQDDRRIFTRDVRLNIVSLQE
                     SLHLDIAKLWQQLNFHQQVQTGSMGDMFAENNALTQTDSAEYQLLMRTLKRFVNAKTL
                     GSLIQVPQEEEAELKVFLDALYRLEQEGDFQQKTAAKAFIPFIQQAWILAQRYDAVVA
                     NPPYMGGNYMETELKNFVSSYYPQGKADLYSSFMVRLLLQLKDNRTLSLMTPFTWMNL
                     SSFEELRKIILTNFSIQSLVQPEYHSFFESAYVPI"
BASE COUNT         2156 a         1864 c         1857 g         1981 t
ORIGIN      
        1 atgcatcttc gttgatatga tagcgactct tttgcttctc tggtatctga tcgataagta
       61 taaccgtacg ttttttaaag tcttcactta atccatttat taaatcatta taacttgcag
      121 ggttaaattg ttttatacaa tcgcgtaata actcaatcgc taacttacgt ttattaagtt
      181 ttttatcttt gcatatatac cacgtatagt ccttctcttt tttctgactg ctaattagct
      241 gctcatcacg tttttcacga aacttaactt gatattcttc caattcagga accggtatga
      301 tttgctctgc attgatcaga acatcatcat taaatctata aggcgtcaga cgaacacatc
      361 gaatgtctac ccctttatct cttaaccaaa tcgcagtggt agtcagttcc tcgctaaaat
      421 ccgatgaagc caaaacgatt ctgatatctt taccaaaatc aacaagctca gtttcatcca
      481 gctcaacaaa ttcaagtatt ttttctttcg cattttttct ttcgcatcgg catcgcggtt
      541 ttgttttttc aaatacgtct ggaaatactc acatgctttc gcaaaactca ttgtgtaaat
      601 cattgccgca tatcgtagtg cctgtaactc catatgcgtc cccgtttcat cacgttttaa
      661 ttcgataacc actatatttg cctgtttatc gattgccaat aaatcaattc gccgatggct
      721 atcttcccag tcagaaatca ccagacaatc aggagcaata atatctatat tttttgattg
      781 cttcctgtaa tccttccaga gtaaaggtcg ttttttctaa agcatgtggt tctttatttg
      841 cgatattgta cagtaccacg ttcatcttcc ttttttaact gattttacag agactttaat
      901 acagttaaaa ttttatttct tgagctgtaa tcgattaagt tgatgcattt aatgggaatg
      961 atatagggtc atttccagtc tcacttatag aaatggctaa agcatgactc tcgccaaaac
     1021 cgtttatgtg ttgtacataa cgcgatcatc cctctcacaa attgcctttt ctcatggcat
     1081 ctcgcccggt cccccattac aatcactttt tgttttttgc gagctgcatt ccagtcttca
     1141 gagggttttt cgatgattaa aaatgacaag gcatggatag gagacttgct gggcggaccg
     1201 ctcatgagca gggaaagccg cgtcattgcc gaactgttgc taaccgatcc cgatgaacag
     1261 acatggcaag agcaaattgt tggccacaac attttacaag cctcttctcc taacaccgca
     1321 aaacgttacg cggcaacaat caggcttcgc ctgaacacgc tggataaaag cgcgtggaca
     1381 ttgattgccg aaggtagtga acgggaacgc caacaacttc tgtttgtggc tctgatgcta
     1441 cattcgccgg tagttaagga ttttctggct gaagtggtga acgatctgcg caggcagttc
     1501 aaggaaaagt tgcctggcaa tagctggaac gaatttgtga atagccaggt tcgcctacat
     1561 ccggtactcg ccagctactc agattcatct attgcaaaaa tgggaaacaa tctggtgaag
     1621 gcgcttgctg aagcgggtta tgtggatacg ccccgcagac gtaacctgca ggcagtttac
     1681 cttttaccgg aaactcaggc agtgttacag cgcctgggac aacaggactt gatatctatt
     1741 ctggagggaa aacggtgata gatcccgttc ttgaatatcg cctgtctcaa atccagagtc
     1801 gcattaacga agatcgcttc ctcaaaaata acggctccgg aaatgaaatt ggtttttgga
     1861 tctttgatta tcccgcgcag tgcgaactgc aggtacggga gcatttgaaa tatctgctcc
     1921 ggcatctgga aaaggaccat aaatttgcct gtctgaatgt cttccaaatc atcatcgata
     1981 tgctcaatga acgcggcctt ttcgagcgcg tctgccagca ggaagtcaaa gtgggtactg
     2041 agacgctgaa aaagcagctt gctggtccgt taaatcagaa aaaaatcgct gattttatag
     2101 cgaaaaaagt cgatctggct gcccaggatt ttgtcattct taccggcatg ggcaacgcct
     2161 ggccattagt acgcggtcat gaactgatga gtgccttgca ggatgtcatg gggttcaccc
     2221 cactgctgat gttttatcct ggcacctaca gcgggtacaa cctttccccg ctcacagaca
     2281 ccggttcaca aaattattat cgcgctttca gactggtacc agatacggga cccgcagcaa
     2341 cattgaatcc tcaatgaaga gcataacaat gaatattgaa cagatttttg aaaaacctct
     2401 aaaacgaaat ataaacgggg tagtcaaagc agagcaaacc gatgatgcca gcgcgtacat
     2461 cgagttagat gaatatgtca tcacccgcga actggaaaac catcttcgcc atttcttcga
     2521 atcctatgtt cctgccactg gcccggaaag gatccgtatg gaaaacaaga tcggcgtatg
     2581 ggtttcaggc ttcttcggtt caggtaaatc gcactttatt aagattcttt cttatctttt
     2641 atctaaccgc aaagttacac ataacggtac ggaacgtaat gcttactcct tctttgaaga
     2701 taaaatcaaa gatgcattat tccttgccga tattaacaaa gcggtgcatt acccgactga
     2761 agtcattctg ttcaatattg attcgcgtgc caacgtagat gacaaagaag atgccattct
     2821 taaagtcttc ctgaaagttt tcaacgaacg cattggctac tgcgctgatt ttccgcatat
     2881 tgcccatctt gagcgcgagc tggataaacg cggtcagtat gaaaccttta aagccgcgtt
     2941 tgccgatatc aatggctcgc gctgggaaga cgagcgcgac gcttactact tcatcagcga
     3001 tgatatggca caagcattaa gccaggccac gcagcagagt cttgaagcct cccgccaatg
     3061 ggtggaacaa ctcgacaaaa acttcccgct ggatatcaat aatttttgcc agtgggtaaa
     3121 agagtggctg gatgacaacg gtaagaacat cctctttatg gtggatgaag tcggtcagtt
     3181 cattggcaaa aatacgcaaa tgatgctgaa gctgcagact attaccgaaa accttggggt
     3241 aatctgcggt ggccgcgcat gggttatcgt gacttcgcag gccgatatca atgcggcaat
     3301 cggtggtatg agcagtcgcg acggacagga cttctccaag atccaggggc gcttctctac
     3361 acgcctgcaa ctttccagct ctaacacatc agaagttatc cagaaacgtt tgttggtaaa
     3421 gactgacgaa gcaaaagcgg cgctggcaaa agtgtggcaa gagaaaggcg atatcctgcg
     3481 taaccagctg gcttttgacc ctacaacaac tactgcacta cgtcctttta ccagcgaaga
     3541 agagttcgtt gacaactacc cgtttgtccc gtggcactat cagattctgc aaaaagtgtt
     3601 tgaatctatt cggacgaaag gtgcagcggg taaacaattg gctatgggtg agcgttctca
     3661 gctggaggca ttccagactg cggcgcagca aatctcagcg caagggctgg attctctggt
     3721 gcctttctgg cgcttctatg ccgccattga gagcttcctg gaacctgccg ttagccgcac
     3781 catcactcag gcttgccaaa atggcattct tgatgagttc gatggcaacc tgcttaaaac
     3841 gctgttcctg atccgctatg tggaaacgct gaaaagcacc ctggataacc tggtcacatt
     3901 atctatcgat aggatcgatg ccgataaagt tgagttgcgc cgccgggtcg aaaaaagtct
     3961 gaacacgctt gaacgcctga tgctcattgc gcgcgttgaa gataaatatg tgttcctgac
     4021 caacgaagag aaagagatcg aaaacgagat ccgtaacgtt gatgtcgatt tctctgcgat
     4081 caacaaaaaa ctggcatcga tcatctttga tgacattctg aaaagccgta aatatcgtta
     4141 tccggctaac aagcaagact ttgatatcag ccgcttcctg aacgggcacc cattagacgg
     4201 cgcagtgctt aacgatctgg tggtgaagat cctgacccct aaagatccga cctatacgtt
     4261 ctataacagt gatgcgacct gtcgccctta tacgtcagaa ggcgacggct gtattttgat
     4321 tcgtctgccc gaagagggcc gtacctggag cgatattgat ttagtcgtcc agactgaaaa
     4381 gttcctcaaa gataacgccg ggcaacgtcc ggaacaggca accctgctct cagaaaaagc
     4441 gcgtgaaaac agcaaccggg aaaaattact ccgtgttcag ttggaatcac tacttgcaga
     4501 agcagacgtc tgggcgattg gcgaacgctt accgaaaaaa tcctccacgc catcgaacat
     4561 tgtcgatgaa gcctgccgtt acgtgattga aaacaccttc ggcaagctga agatgctgcg
     4621 gccttttaac ggtgacatct cccgtgaaat tcatgcatta ctgacggttg agaacgacac
     4681 cgaactggat ctcggtaacc tcgaagagtc caaccccgac gccatgcgcg aggtagaaac
     4741 ctggatcagc atgaatatcg aatacaataa acctgtgtat ttacgcgata ttctgaacca
     4801 ttttgcgcgt cgcccttatg gctggcccga agacgaagtg aaactgctag tagcccgtct
     4861 ggcctgcaaa ggtaaattca gcttcagcca gcaaaacaac aacgtcgagc gaaaacaggc
     4921 gtgggagtta tttaataaca gccgccgcca tagcgaattg cgtctgcata aagttcgccg
     4981 tcatgatgaa gcgcaggtgc gtaaagccgc gcaaaccatg gctgacatcg ctcagcagcc
     5041 gtttaacgaa cgggaagagc cggcgctggt tgaacatatt cgtcaggtat ttgaagagtg
     5101 gaagcaagag ctgaacgtat tccgcgccaa ggcagagggc ggaaacaatc cggggaaaaa
     5161 cgagattgaa tccggtctgc gcctgcttaa tgccattctt aatgagaaag aagattttgc
     5221 cctgatcgaa aaagtctcat cgctgaaaga tgaacttctg gatttcagcg aagaccgtga
     5281 agatttggtc gacttctacc gtaagcaatt cgccacctgg caaaaactgg gtgctgcgct
     5341 gaatggcagc tttaaatcta accgcagcgc gctggaaaaa gacgccgcag cggttaaagc
     5401 gctgggcgag ctggaaagca tctggcaaat gccggaacct tataagcatc tcaatcgcat
     5461 cacgccgttg attgaacagg tccagaacgt caaccatcag ttagtcgaac agcatcgcca
     5521 gcacgccctc gaacgcattg acgcccgcat tgaggaaagc cgtcaacgct tgctggaagc
     5581 gcacgccacg tcggagctgc aaaacagcgt tctgctgccg atgcaaaaag ccagaaaacg
     5641 cgctgaagtc agccagtcga ttccggaaat tttggcggaa cagcaagaga caaaagcgct
     5701 gcaaatggat gcagataaaa agattaacct gtggatcgac gagctgcgta aaaagcaaga
     5761 agcacaactc cgggcagcaa atgaagctaa acgcgctgcc gactcagaac agacttatgt
     5821 tgtggtggaa aaaaccgtta tccaaccggt accgaaaaaa acgcatctgg tgaatgtcgc
     5881 cagtgagatg cgtaatgcca ccggtggtga agttctggaa acgaccgaac aggtggaaaa
     5941 ggcgctcgac acgttacgca caacgctgct ggccgtcatt aaagcaggcg atcgcattcg
     6001 ccttcagtaa ctcccatttc agggcagcac tctgctgccc tttgcaggat tttctatgaa
     6061 taccaataac attaaaaaat atgccccaca ggcccgtaac gacttccgcg atgcggtggt
     6121 ccagaagcta acgacgcttg ggatcgctgc agataaaaaa ggcaatttgc agattgccga
     6181 ggccgaaacc attggcgaga ccgtgcgtta cggtcagttt gattacccgt tatcgaccct
     6241 tccccgccgc gaacggctgg taaaacgcgc ccgtgagcag ggttttgagg tgctggttga
     6301 gcactgcgcc tacacctggt ttaaccgctt atgtgcaatt cgctatatgg agctacacgg
     6361 ttatcttgag cacggcttcc gtatgttgtc ccacccggag acgccgaccg cgtttgaggt
     6421 gctggatcat gtgccggaag tggcagaagc cctgctgccg gaaaataagg cgcagctggt
     6481 tgaaatgaag ctttccggta atcaggacga agccctgtac cgcgaactgc tgctggggca
     6541 gtgccacgcc ctgcaccacg cgatgccgtt cctgtttgaa gcggtagatg acgaagcgga
     6601 actgctgttg ccggataacc tgacccgtac cgactctatt ctgcgtgggc tggttgatga
     6661 tattccggaa gaagactggg agcaggtaga ggttatcggc tggctgtatc agttctatat
     6721 ttcggaaaag aaagatgccg tgattggcaa agtggtgaag agcgaagata ttcctgccgc
     6781 cacccagctg tttacgccaa actggattgt gcagtatctg gtacaaaact ccgttggccg
     6841 ccagtggttg cagacctacc cggactcgcc gctgaaagac aaaatggagt actacatcga
     6901 gcctgcggaa caaacgccgg aagtgcaggc gcagctggcg gcgattaccc cagccagcat
     6961 tgaacccgaa agtattaaag tgctcgaccc agcctgcggc tccggtcata ttttgattga
     7021 agcctataat gtgctgaaaa atatctacga agagcgtggt tatcgcgggc gtgatattcc
     7081 acaactgatt ctggaaaata atatttttgg tcttgatatc gacgaccgcg cggcacagct
     7141 ttccggcttt gcattattaa tgatggcgag tcaggatgac cgcagaatat ttacccgcga
     7201 tgtacgtctg aatattgtct ctttgcagga aagcctgcat ctggatatcg ccaaactctg
     7261 gcagcaactg aatttccacc agcaggtaca aaccggcagt atgggggata tgtttgctga
     7321 aaataacgcg ttaacccaaa ctgacagcgc agaatatcag ctgctgatgc gcacgctgaa
     7381 acgctttgtg aatgcaaaaa cgctgggctc actgattcag gtgccgcagg aagaagaagc
     7441 ggaactgaag gtattcctgg acgcgttgta tcgcctggaa caggaaggcg atttccagca
     7501 gaagacggcg gcaaaagcgt ttattccgtt tattcagcag gcgtggattt tagcgcagcg
     7561 atatgatgcg gtagtggcga atccgccgta tatggggggt aattatatgg agacagaact
     7621 taagaatttc gtctcttctt actaccctca aggaaaggcg gatctttatt cttcatttat
     7681 ggtcagatta cttttacaat taaaagataa tcgcacttta agcctaatga ccccctttac
     7741 ttggatgaat ttatcatcat ttgaagagct ccgaaaaatt atacttacaa atttcagcat
     7801 tcagtcatta gtacagcctg aatatcattc attttttgag tcagcttatg tcccaatt
//
LOCUS       DAHHTG010000176         7534 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.179, whole genome shotgun sequence.
ACCESSION   DAHHTG010000176 DAHHTG010000000
VERSION     DAHHTG010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7534)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7534)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7534
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.179"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            238..4485
                     /gene="fdeC"
                     /locus_tag="NBI74_004131"
     CDS             238..4485
                     /gene="fdeC"
                     /locus_tag="NBI74_004131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000092542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intimin-like adhesin FdeC"
                     /protein_id="HCD1934379.1"
                     /translation="MSRYKTDNKQPRFRYSVLARCVAWANISVQVLFPLAVTFTPVMA
                     ARAQHAVQPRLSMENTTVTANNNVEKNVASLAANAGTFLSSQPDSDATRNFITGMATA
                     KANQEIQEWLGKYGTARVKLNVDKNFSLKDSSLEMLYPIYDTPTNMLFTQGAIHRTDD
                     RTQSNIGFGWRHFSENDWMAGVNTFIDHDLSRSHTRIGVGAEYWRDYLKLSANGYIRA
                     SGWKKSPDVEDYQERPANGWDIRAEGYLPAWPQLGASLMYEQYYGDEVGLFGKDKRQK
                     DPHAITAEVNYTPVPLLTLSAGHKQGKSGENDTRFGLEVNYRIGEPLEKQLDTDSIRE
                     RRMLAGSRYDLVERNNNIVLEYRKSEVIRIALPERIEGKGGQTVSLGLVVSKATHGLK
                     NVQWEAPSLLAAGGKITGQGNQWQVTLPAYQAGKDNYYAISAIAYDNKGNASKRVQTE
                     VVISGAGMSADRTALTLDGQSRIQMLANGNEQKPLVLSLRDAEGQPVTGMKDQIKTEL
                     TFKPAGNIVTRTLKATKSQAKPTLGEFTETESGVYQSVFTTGTQSGEATITVSVDDMS
                     KTVTAELRATMMDVANSTLSANEPSGDVVADGQQAYTLTLTAVDSEGNPVTGEASRLR
                     LVPQDTNGVTVGAISEIKPGVYSATVSSTRAGNVVVRAFSEQYQLGTLQQTLKFVAGP
                     LDAAHSSITLNPDKPVVGGTVTAIWTAKDANDNPVTGLNPDAPSLSGAAAAGSTASGW
                     TDNGDGTWTAQISLGTTAGELDVMPKLNGQDAAANAAKVTVVADALSSNQSKVSVAED
                     HVKAGESTTVTLVAKDAHGNAISGLSLSASLTGTASEGATVSSWTEKGDGSYVATLTT
                     GGKTGELRVMPLFNGQPAATEAAQLTVIAGEMSSANSTLVADNKTPTVKTTTELTFTV
                     KDAYGNPVTGLKPDAPVFSGAASTGSERPSAGNWTEKGNGVYVSTLTLGSAAGQLSVM
                     PRVNGQNAVAQPLVLNVAGDASKAEIRDMTVKVNNQLANGQSANQITLTVVDSYGNPL
                     QGQEVTLTLPQGVTSKTGNTVTTNAAGKVDIELMSTVAGEHNISASVNGAQKTVTVKF
                     NADASTGQANLQVDTAVQKVANGKDAFTLTANVEDKNGNPVPGSLVTFNLPRGVKPLT
                     GDNVWVKANDEGKAELQVVSVTAGTYEITASAGNDQPSNAQSVTFVADKTTATISSIE
                     VIGNRAVADGKTKQTYKVTVTDANNNLLKDSEVTLTASPENLVLTPNGTATTNEQGQA
                     IFTATTTVAATYTLTAKVEQADGQESTKTAESKFVADDKNAELAATSDVHSLVADGVT
                     TATLTVTLFSANNPVGGTMWVDIEAPEGVTEADYQFLPSKNDHFASGKITRTFSTNKP
                     GTYTFTFNSLTYGGYEMKPVTVTINAVPADTEGAEEK"
     gene            complement(4610..5467)
                     /locus_tag="NBI74_004132"
     CDS             complement(4610..5467)
                     /locus_tag="NBI74_004132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCD1934380.1"
                     /translation="MIRQKILQQLLEWIECNLEHPISIEDIAQKSGYSRRNIQLLFRN
                     FMHVPLGEYIRKRRLCRAAILVRLSAKSMLDIALSLHFDSQQSFSREFKKLFGCSPRE
                     YRHRDYWDLANIFPSFLIRQQQKTECRLVNFPETPIFGNSFKYDIEVSNKLPDEEVKL
                     RRHHLVRCMKNFKTDIYFVSTFEPSTKSVDLLTVETFAGTVCKQTDSEMPKEWTINRG
                     LYASFRYEGEWEHYPEWARNLYLMELPARGLARVNGSDIERFYYNENFVEIDSNNIVC
                     EIFIPVRPV"
     gene            5715..6584
                     /locus_tag="NBI74_004133"
     CDS             5715..6584
                     /locus_tag="NBI74_004133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308365.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HCD1934381.1"
                     /translation="MEFSVLSNNLKMPMMGFGVFQVTDKDVCKQSVLNAIRTGYRLID
                     TAAVYGNEDAVGEAVREAISEGLCTREELFITSKLWVQDMLNQDIAAAGIEASLKKSG
                     LEYFDLYLLHQAMRDYFSAWRALEDAYEEGKLKAIGVSNFYPHVLANFCETVRVKPMV
                     NQVELHPYFAQPEALATMKYYNVQPEAWAPLGGGRHKPFENNLLQSIADAHQKSISQV
                     ILRWNIQRGVVVIPKSTHQQRIEENFAIWDFSLTEKEMAQISSLDLGYVGESVKHFNP
                     EFVRGCLAVKIHD"
     gene            complement(6744..7337)
                     /gene="rclC"
                     /locus_tag="NBI74_004134"
     CDS             complement(6744..7337)
                     /gene="rclC"
                     /locus_tag="NBI74_004134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reactive chlorine species resistance protein
                     RclC"
                     /protein_id="HCD1934382.1"
                     /translation="MERYLHLLSRGDKIGLTLIRLSIAIVFMWIGLLKFVPYEADSIT
                     PFVANSPLMSFFYEHPEDYKQYLTHEGEYKPEARAWQTANNTYGFSNGLGVVEVIIAL
                     LVLANPVNRWLGLLGGLMAFTTPLVTLSFLITTPEAWVPALGDAHHGFPYLSGAGRLV
                     LKDTLMLAGAVMIMADSARDILKQRSNESSSTLKTEY"
     gene            complement(7349..>7534)
                     /locus_tag="NBI74_004135"
     CDS             complement(7349..>7534)
                     /locus_tag="NBI74_004135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HCD1934383.1"
                     /translation="FSTNADDKIILKHISVSSVSASPTVLENAIADMARKYNASSWKV
                     TSMRIDNNSTATAVLYK"
BASE COUNT         2075 a         1677 c         1927 g         1855 t
ORIGIN      
        1 atgacttgca caaaaagcta aatttttacc cataaataaa aatataatcc cgcgcccaac
       61 cacctgatga gtggctacag acactggata tattaggtgg cggtgtactt tcttacataa
      121 aggtatttcc ttttctgtgg aaaaggaaac cgggaaatcc ccggtttttc tgacaagcag
      181 acgccattat ttgtgtctgc ctatgttcgt taattcgttc atcaggaaat tatctcaatg
      241 tcacgttata aaacagacaa taaacagcca cgatttcgtt attcagttct ggcccgctgc
      301 gtggcgtggg caaatatctc tgttcaggtt ctttttccac tcgcagtcac ctttacgcca
      361 gtaatggcag cacgtgcgca gcatgcggtt cagccacggt tgagcatgga aaatactacg
      421 gtaactgcta ataataacgt ggagaaaaat gtcgcgtcgc ttgccgctaa tgccgggaca
      481 tttttaagca gtcagccaga tagcgatgcg acacgtaact ttattaccgg aatggccacc
      541 gctaaagcta accaggaaat tcaggagtgg ctcgggaaat acggtactgc gcgcgtcaaa
      601 ctgaatgtcg ataaaaattt ctcgctgaag gactcttcgc tggaaatgct ttatccgatt
      661 tatgatacac cgacaaatat gttgttcact cagggggcaa tacatcgtac cgacgatcgt
      721 actcagtcaa atattggttt tggctggcgt catttttcag aaaatgactg gatggcgggg
      781 gtgaatactt ttatcgatca tgatttatcc cgtagtcata cccgcattgg tgttggtgcg
      841 gaatactggc gtgattattt gaaactgagc gccaatggtt atatccgggc ttctggctgg
      901 aaaaaatcgc cggatgttga ggattatcag gaacgcccgg cgaatggctg ggatattcgt
      961 gctgagggct atttacctgc ctggccgcag cttggcgcaa gcctcatgta tgaacagtat
     1021 tatggcgatg aagtcgggct gtttggtaaa gataaacgcc agaaagatcc acatgcgatt
     1081 accgctgaag tgaattacac gccagtgcct cttctgaccc tgagtgccgg gcataagcag
     1141 ggcaagagtg gtgagaatga cactcgcttt ggcctggaag ttaattatcg gattggcgaa
     1201 cctctggaaa aacaactcga tacagacagc attcgcgagc gtcgaatgct ggcaggcagc
     1261 cgctatgacc tggttgagcg taataacaat atcgttcttg agtatcgcaa atctgaagtg
     1321 atccgtattg ctctgcctga gcgtattgaa ggcaagggcg gccagacggt ttccctgggg
     1381 cttgtggtca gcaaagcaac tcacggtctg aaaaatgtgc aatgggaagc gccgtctttg
     1441 ctggccgcag gcggaaaaat tacggggcag ggcaatcagt ggcaagtgac gctcccggct
     1501 tatcaggcag gcaaagacaa ttattatgcg atttcagcga ttgcctacga taacaaaggc
     1561 aatgcctcga aacgtgtgca gacagaagtg gttattagcg gagctggtat gagcgccgat
     1621 cgtacggcgt taacgcttga cggtcagagc cgtattcaaa tgcttgctaa cggtaatgag
     1681 caaaagccgc tggtgctgtc tctgcgcgac gccgagggtc agccagtcac gggcatgaaa
     1741 gatcagatca agactgaact aaccttcaaa ccggctggaa atattgtgac tcgtaccctg
     1801 aaggccacta aatcacaggc aaagccaaca ctgggtgagt tcaccgaaac tgaatcaggg
     1861 gtgtatcagt ctgtctttac taccggaacg cagtcaggtg aggcaacgat tactgttagc
     1921 gttgatgaca tgagcaaaac tgtcactgca gaactgcggg ccacgatgat ggatgtggca
     1981 aactccaccc tgagcgctaa cgagccgtca ggtgatgtgg ttgctgatgg tcagcaagcc
     2041 tacacgctga cactgacagc ggtggactcc gagggtaatc cggtgacggg agaagccagc
     2101 cgcctgcgac ttgttccgca agacactaat ggtgtaaccg ttggtgccat ttcggaaata
     2161 aaaccagggg tttacagcgc cacggtttct tcgacccgtg ccgggaacgt tgttgtgcgt
     2221 gccttcagcg agcagtatca gctgggcacg ttacaacaaa cgctgaagtt tgttgccggg
     2281 ccgcttgatg cagcacattc gtccatcacc ctgaatcctg ataaaccggt ggttggcggt
     2341 acagttacgg caatctggac ggcaaaagat gctaatgaca accctgtaac tggcctcaat
     2401 ccggatgcac cgtcattatc gggcgcagct gctgctggtt ctacggcatc aggctggacg
     2461 gataatggcg acgggacctg gactgcgcag atttctctcg gcactacggc gggtgaatta
     2521 gacgttatgc cgaagctcaa tgggcaggac gcggcagcga atgcggcaaa agtaaccgtg
     2581 gtggctgatg cattatcttc aaaccagtcg aaagtctctg tcgcagaaga tcacgtaaaa
     2641 gccggtgaaa gcacaaccgt aacgctggtg gcgaaagatg cgcatggcaa cgctatcagt
     2701 ggtctttcgt tgtcggcaag tttgacgggg accgcctctg aaggggcgac cgtttccagt
     2761 tggaccgaaa aaggtgacgg ttcctatgtt gctacgttaa ctacaggcgg aaagacgggc
     2821 gagcttcgtg tcatgccgct cttcaacggc cagcctgcag ccaccgaagc cgcgcagctg
     2881 actgttattg ccggagagat gtcatcagcg aactctacgc ttgttgcgga caataaaact
     2941 ccaacggtta aaacgacgac ggaactcacc ttcaccgtga aggatgcgta cgggaatccg
     3001 gtcaccgggc tgaagccaga tgcaccagtg tttagtggtg ccgccagcac ggggagtgag
     3061 cgtccttcag caggaaactg gacagagaaa ggtaatgggg tctacgtgtc gaccttaacg
     3121 ctgggatctg ccgcgggtca gttgtctgtg atgccgcgag tgaacggcca aaatgccgtt
     3181 gctcagccac tggtgctgaa tgttgcaggt gacgcatcta aggctgagat tcgtgatatg
     3241 acagtgaagg ttaataacca actggctaat ggacagtctg ctaaccagat aaccctgacc
     3301 gtcgtggaca gctatggtaa cccgttgcag gggcaagaag ttacgctgac tttaccgcag
     3361 ggtgtgacca gcaagacggg gaatacagta acaaccaatg cggcagggaa agtggacatt
     3421 gagcttatgt caacggttgc gggagaacac aatatttccg cttcggtgaa tggtgctcag
     3481 aagacggtca cggtgaaatt caacgcggat gccagcaccg gtcaggcaaa cctgcaggta
     3541 gacaccgccg ttcaaaaagt ggcaaacggc aaagatgcct ttacgctgac ggcgaacgtt
     3601 gaggataaaa atggtaaccc tgttccaggg agcctggtga cctttaatct gccccggggt
     3661 gtcaagccgc ttacaggcga taatgtgtgg gtgaaagcca acgatgaggg gaaagcggag
     3721 ttgcaggtgg tttccgtgac tgccggaacc tatgagatca cggcgtcagc aggaaatgac
     3781 cagccttcga atgcgcagtc tgtaacgttt gtggccgata agactacggc gaccatctcc
     3841 agtattgagg tgattggcaa ccgtgcagtg gcggacggca aaaccaaaca gacgtataaa
     3901 gttacggtga ctgatgccaa taacaacctg ctgaaagata gcgaagtgac gctgactgcc
     3961 agcccggaaa atttagttct gactcccaat gggacggcga caacgaatga gcaagggcag
     4021 gctattttca ccgccacgac cactgtcgca gcgacatata cactcacggc gaaagtggaa
     4081 caggccgacg gtcaggaatc gacgaaaact gccgaatcta aattcgtcgc ggatgataaa
     4141 aacgcggagc tcgccgcaac atctgatgtg cattctctgg tggcggatgg ggtaacgaca
     4201 gcaacactga cggtaaccct gttctcggca aacaacccgg taggaggaac catgtgggtc
     4261 gacattgagg ctccggaagg ggtgacagag gcggattatc agttcctgcc gtcgaaaaat
     4321 gaccatttcg cgagcgggaa aatcacgcgt acatttagta ccaacaagcc aggtacatac
     4381 acattcacat tcaactcttt gacatatgga gggtatgaaa tgaaacccgt gactgtgaca
     4441 attaacgccg ttcctgcaga tactgaaggt gctgaggaga aataaaaacg acgatgtaat
     4501 aaataataat ggcgttgaca tgcgcagcgc cattatttac tgaataccta ccctggcacc
     4561 gattagacgg tggcagggta gggtaaatct tatgtgatgg agttacaggc taaaccgggc
     4621 gaacggggat aaaaatttcg caaacaatgt tattgctatc aatttcgacg aagttttcat
     4681 tgtaataaaa tcgctcaata tcactgccgt tcactctggc taaccccctg gcaggtaatt
     4741 ccatcagata gaggtttcgc gcccattcag ggtagtgctc ccactcacct tcatagcgga
     4801 aagaggcata cagtcctcgg ttaattgtcc actctttcgg catctctgaa tcggtttgtt
     4861 tgcataccgt accggcaaaa gtttcaactg tgagcaaatc gaccgatttt gttgatggtt
     4921 cgaacgtgga gacgaaatag atatccgtct taaaattctt catacatctg actaaatgat
     4981 gacgtcgcag tttgacttct tcatccggta atttattcga cacttcaata tcatatttaa
     5041 atgaattgcc aaaaataggt gtctcaggaa agttgactaa tctacactcc gtcttttgct
     5101 gttgacgtat taaaaaagaa gggaagatat ttgcgagatc ccaataatca cggtggcggt
     5161 attcacgggg agagcagccg aataactttt taaattcgcg gctgaatgac tgctgtgaat
     5221 caaaatgcaa agagagtgca atatcaagca tagatttcgc ggagagccgg acaagaatgg
     5281 cggcacgaca aagccttcgt ttgcgaatgt attctcccaa aggcacatgc atgaaatttc
     5341 ggaacagaag ctggatgttg cgtctgctgt agccagattt ctgtgcgata tcttcgattg
     5401 aaatagggtg ctcaagattg cactcaatcc actccaggag ctgctgtaga atcttctgcc
     5461 tgatcatcac atactccgtt aattctttgt catatcgcac attttcgtta tgtcctgggc
     5521 cctaagtgta tcaaaaacag caagtcccaa ggcaaaagca ctgatagcgt ctgcttcatt
     5581 gccccaaaaa tagagaggtt tcagtcctac attattaatg aattttttgc ataagtgata
     5641 tcaaaatcca catactaatt tgaggttacg ttttaacgta gactcattgt tcatacctaa
     5701 tggagggact gacagtggaa ttttccgtat taagtaacaa tctgaaaatg ccgatgatgg
     5761 gatttggtgt ttttcaggtt accgataaag acgtgtgcaa acagtcagtg ttgaatgcta
     5821 tccgcacggg ttatcgactc atcgacactg cagcggtata cggtaatgaa gatgccgtcg
     5881 gtgaagccgt tcgtgaagcc atatctgaag gtttatgtac ccgagaagag ttatttataa
     5941 cgtctaaatt gtgggtgcag gatatgttga atcaggatat tgcagcagca ggtattgaag
     6001 catcattaaa aaaatcgggg ctagaatact tcgatctcta tttattgcac caggccatgc
     6061 gcgattattt tagtgcgtgg cgtgcactcg aagatgccta tgaagagggc aaattaaaag
     6121 cgattggggt ttccaatttc taccctcatg ttctggcgaa cttttgtgaa acggtaagag
     6181 ttaaaccgat ggtcaaccag gtcgagttgc atccctattt tgcccaacca gaggcgctgg
     6241 caaccatgaa gtattataac gtgcagcctg aagcatgggc tccgttaggt ggtggacgac
     6301 ataaaccctt tgaaaataat ctgcttcaga gtattgcaga tgcccatcaa aaatcgattt
     6361 ctcaggtcat tctgcgttgg aatattcaac ggggagtggt cgttattccg aaatcgacac
     6421 atcaacagcg tatcgaagaa aattttgcta tctgggattt ctcactgaca gagaaagaaa
     6481 tggcgcaaat tagttcgcta gatttgggtt atgttgggga atcggtaaaa cattttaatc
     6541 ctgagtttgt tcgtggttgt cttgctgtaa aaatacatga ttgatattaa tcacttattt
     6601 tacctgagac gacaaaaatt ttttaacagg ggagtgatat tgatcgtcac tctgtcatat
     6661 ctccggtaat atggcgtcag gcttcatcaa cagaaagggg gcctgacgcc tgaaaaagtg
     6721 aacaacagac agtgttcgga ttatcaatat tcagttttta acgttgaact ggattcatta
     6781 ctgcgttgtt taagaatatc ccgtgccgaa tctgccatta tcatgacagc acctgccagc
     6841 atcagagtat ctttcaatac cagacgacca gcaccggata aataagggaa accatgatga
     6901 gcatcaccca atgcgggtac ccatgcctcc ggagtggtga ttaaaaatga gagtgttacc
     6961 agcggcgtgg taaatgccat cagcccaccc aataaaccta accagcgatt gacaggatta
     7021 gccaaaacca gcagagcaat aatcacctcc acgacgccaa gaccgttgga aaaaccatag
     7081 gtattattag ccgtttgcca tgctcttgct tctggtttgt attcgccttc gtgagtcaga
     7141 tactgtttat agtcttccgg gtgttcataa aagaacgaca ttagtggact gtttgcgacg
     7201 aatggtgtaa tgctgtctgc ctcgtaaggg acaaacttta ataacccaat ccacataaaa
     7261 acaatcgcaa tgctcagacg aatcaatgtc aggccaattt tatctccccg acttaacagg
     7321 tgcaggtatc tttccattgt aaacatcctt atttatacaa tactgctgtt gcggttgaat
     7381 tattatcaat tcgcatcgat gtaactttcc aggatgaagc attatatttt ctggccatat
     7441 cagcaatggc attctcaaga actgtcggtg atgctgatac tgatgagacg ctgatatgtt
     7501 tcagaattat tttatcatct gcattggtgg aaaa
//
LOCUS       DAHHTG010000177         7484 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.180, whole genome shotgun sequence.
ACCESSION   DAHHTG010000177 DAHHTG010000000
VERSION     DAHHTG010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7484)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7484)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7484
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.180"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            119..550
                     /gene="osmC"
                     /locus_tag="NBI74_004136"
     CDS             119..550
                     /gene="osmC"
                     /locus_tag="NBI74_004136"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin OsmC"
                     /protein_id="HCD1934384.1"
                     /translation="MTIHKKGQAHWEGDIKRGKGTVSTESGVLNQQPYGFNTRFEGEK
                     GTNPEELIGAAHAACFSMALSLMLGEAGFTPTSIDTTADVSLDKVDAGFAITKIALKS
                     EVAVPGIDASTFDGIIQKAKAGCPVSQVLKAEITLDYQLKS"
     gene            complement(740..3139)
                     /gene="dosP"
                     /locus_tag="NBI74_004137"
                     /gene_synonym="pdeO"
     CDS             complement(740..3139)
                     /gene="dosP"
                     /locus_tag="NBI74_004137"
                     /gene_synonym="pdeO"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-sensing cyclic-di-GMP phosphodiesterase"
                     /protein_id="HCD1934385.1"
                     /translation="MKLTDADNAADGIFFPALEQNMMGAVLINENDEVMFFNPAAEKL
                     WGYKREEVIGNNIDMLIPRDLRPAHPQYIRHNREGGKARVEGMSRELQLEKKDGSKIW
                     TRFALSKVSAEGKVYYLALVRDASVEMAQKEQTRQLIIAVDHLDRPVIVLDPERHIVQ
                     CNRAFTEMFGYCINEASGMQPDTLLNIPEFPADNRIRLQQLLWKTARDQDEFLLLTRT
                     GEKIWIKASISPVYDVLAHLQNLVMTFSDITEERQIRQLEGNILAAMCSSPPFHEMGE
                     IICRNIESVLNESHVSLFALRNGMPIHWASSSHGAEVQNAQSWSATIRQRDGAPAGIL
                     QIKTSSGAETSAFIERVADISQHMAALALEQEKSRQHIEQLIQFDPMTGLPNRNNLHN
                     YLDDLVDKAVSPVVYLIGVDHIQDVIDSLGYAWADQALLEVVNRFREKLKPDQYLCRI
                     EGASFVLVSLENDVSNITQIADELRNVVSKPIMIDDKPFPLTLSIGISYDVGKNRDYL
                     LSTAHNAMDFIRKNGGNGWQFFSPAMNEMVKERLVLGAALKEAISNNQLKLVYQPQIF
                     AETGELYGIEALARWYDPLHGHVPPSRFIPLAEEIGEIENIGRWVIAEACRQLAEWRS
                     QNIHIPALSVNLSALHFRSNQLPNQVSDAMHAWGIDGHQLTVEITESMMMEHDTEIFK
                     RIQILRDMGIGLSVDDFGTGFSGLSRLVSLPVTEIKIDKSFVDRCLTEKRILALLEAI
                     TSIGQSLNLTVVAEGVETKEQFEMLRKIHCRVIQGYFFSRPLPAEEIPGWMSSVLPLK
                     I"
     gene            complement(3164..3327)
                     /locus_tag="NBI74_004138"
                     /pseudo
     CDS             complement(3164..3327)
                     /locus_tag="NBI74_004138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310122.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
     gene            complement(3705..5024)
                     /locus_tag="NBI74_004139"
     CDS             complement(3705..5024)
                     /locus_tag="NBI74_004139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 10 protein"
                     /protein_id="HCD1934386.1"
                     /translation="MDICSRNEKLAIRRPAILVALALLLCSCKSTPPESMVTPPAGSK
                     PPATTQQSSQPMRGIWLATVSRLDWPPVSSVNNSNPTSRARVQQQAMIDKLDHLQRLG
                     INTVFFQVKPDGTALWPSKILPWSDLMTGKIGENPGYDPLQFMLDEAHKRGMKVHAWF
                     NPYRVSVNTKPGTIRELNSTLSQQPASVYVQHRDWIRTSGDRFVLDPGIPEVQDWITS
                     IVAEVVSRYPVDGVQFDDYFYTESPGSRLNDNETYRKYGGAFASKADWRRNNTQQLIA
                     KVSHTIKSIKPEVEFGVSPAGVWRNRSHDPLGSDTRGAAAYDESYADTRRWVEQGLLD
                     YIAPQIYWPFSRSAARYDVLAKWWADVVKPTRTRLYIGIAFYKVGEPSKIEPDWMING
                     GVPELKKQLDLNDAVPEISGTILFREDYLNKPQTQQAVSYLQSRWGS"
     gene            complement(5155..6690)
                     /gene="gadC"
                     /locus_tag="NBI74_004140"
     CDS             complement(5155..6690)
                     /gene="gadC"
                     /locus_tag="NBI74_004140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance gamma-aminobutyrate antiporter
                     GadC"
                     /protein_id="HCD1934387.1"
                     /translation="MATSVQTGKAKQLTLLGFFAITASMVMAVYEYPTFATSGFSLVF
                     FLLLGGILWFIPVGLCAAEMATVDGWEEGGVFAWVSNTLGPRWGFAAISFGYLQIAIG
                     FIPMLYFVLGALSYILKWPALNEDPITKTIAALIILWALALTQFGGTKYTARIAKVGF
                     FAGILLPAFILIALAAIYLHSGAPVAIEMDAKTFFPDFSKVGTLVVFVAFILSYMGVE
                     ASATHVNEMSNPGRDYPLAMLLLMVAAICLSSVGGLSIAMVIPGNEINLSAGVMQTFT
                     VLMSHVAPEIEWTVRVISALLLLGVLAEIASWIVGPSRGMYVTAQKNLLPAAFAKMNK
                     NGVPVTLVISQLVITSIALIILTNTGGGNNMSFLIALALTVVIYLCAYFMLFIGYIVL
                     VLKHPDLKRTFNIPGGKGVKLVVAIVGLLTSIMAFIVSFLPPDNIQGDSTDMYVELLV
                     VSFLVVLALPFILYAVHDRKSKANTGVTLEPINSQNAPKGHFFLHPRARSPHYIVMND
                     KKH"
     gene            complement(6846..>7484)
                     /locus_tag="NBI74_004141"
     CDS             complement(6846..>7484)
                     /locus_tag="NBI74_004141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HCD1934388.1"
                     /translation="APDIVWDFRLPRVKSISASGHKFGLAPLGCGWVIWRDEEALPQE
                     LVFNVDYLGGQIGTFAINFSRPAGQVIAQYYEFLRLGREGYTKVQNASYQVAAYLADE
                     IAKLGPYEFICTGRPDEGIPAVCFKLKDGEDPGYTLYDLSERLRLRGWQVPAFTLGGE
                     ATDIVVMRIMCRRGFEMDFAELLLEDYKASLKYLSDHPKLQGIAQQNSFKHT"
BASE COUNT         1919 a         1841 c         1866 g         1858 t
ORIGIN      
        1 atttaattta agtgcagaaa ttatattttc gccggatttt attcggatta tcctgcttat
       61 cctcgtgctg tttctcacgt agtctataat ttccttttaa gcccacagga gagcaacaat
      121 gacaatccat aagaaaggtc aggcacattg ggaaggcgat atcaaacgcg ggaagggaac
      181 agtatctact gagagcggcg tgctgaacca acagccgtat ggatttaaca cgcgttttga
      241 aggcgaaaaa ggaaccaacc ctgaagaact gattggcgca gcgcatgccg catgtttctc
      301 aatggcgctt tcattaatgc tgggggaagc gggattcacg ccaacatcga ttgataccac
      361 cgccgatgtc tcgctggata aagtggatgc cggttttgcg attactaaaa tcgcactgaa
      421 gagcgaagtt gcggtgccgg gtattgatgc ctccaccttt gacggcatta tccagaaagc
      481 aaaagcagga tgcccggttt ctcaggtact gaaagcggaa attacgctgg attaccagtt
      541 gaaatcgtaa agcgttgccg gatgacgcgt caggcgcgtg aatgcctgat gcgtcagcac
      601 aatcacatca ggctgagaag ggattgttct gacatggcta tgcgttgtcg ttgtccaact
      661 aaacgtagcg gaaatcacag catttaagtg acagtgtcac gttaaataaa aacccgcgag
      721 tgcgggcgag aggaatttat cagattttca gcggtaacac gctgctcatc cagcctggaa
      781 tttcttcggc gggcaggggg cgggaaaaga aatatccctg aataacgcga cagtggatct
      841 tgcgtagcat ctcaaattgc tctttggttt cgacgccttc cgccacgacg gttaaattga
      901 ggctttgccc aatgctggta atggcttcga gtaaggcaag gatgcgtttt tcagtcagac
      961 aacgatcgac aaaacttttg tcaattttga tttccgttac cggaagactg actaagcggg
     1021 ataatccgga aaagcccgta ccaaaatcat ctaccgataa accgatgccc atatcgcgca
     1081 ggatctgaat gcgcttaaag atttcggtat cgtgttccat catcatgctt tccgtgattt
     1141 ccaccgtcag ctggtggccg tcaatacccc aggcgtgcat tgcatcagat acctgattag
     1201 gcagctgatt actgcgaaag tgcagcgcag acaagttaac ggataacgcc gggatatgaa
     1261 tattctggct acgccattct gctaactgac ggcaagcttc cgcgatgacc cagcgcccaa
     1321 tattttcgat ttcaccaatc tcttctgcga gaggaataaa ccgtgaaggg ggcacatgac
     1381 catgcagggg atcgtaccag cgagcaaggg cttcgatgcc gtacagttca cccgtttctg
     1441 cgaagatttg cggctgataa accagtttaa gttggttatt gctaatcgct tcttttagcg
     1501 ctgcgcctaa aaccaaacgc tcttttacca tttcgttcat cgccgggctg aagaactgcc
     1561 agccgttacc gccatttttg cgaataaaat ccattgcatt gtgagcagtg gagagcaggt
     1621 aatcgcggtt tttacccacg tcgtagctga tgccaatgct caaggtgagc gggaatggtt
     1681 tatcgtcaat cattatcggc ttgctgacca cattccgtag ctcatcggcg atctgggtaa
     1741 tgttactgac atcgttttcc aggctcacga ggacaaaaga cgcaccttca atacgacaga
     1801 gatactgatc cggtttgagt ttttcacgaa agcgattgac cacttccagc aatgcctgat
     1861 cggcccacgc atagccaagg ctatcaatca catcctgaat atggtcaaca ccgatgagat
     1921 acaccaccgg agagacggct ttgtcgacca ggtcatcgag gtaattgtgc aggttattgc
     1981 gatttggcag accggtcatc ggatcaaatt ggatgagttg ttcaatatgc tgacggcttt
     2041 tttcctgttc cagcgccagc gcggccatat gctggctgat atctgccacg cgttcgataa
     2101 aggcgctggt ttctgctcct gacgaggttt taatttgcag gatccccgca ggcgcgccat
     2161 cgcgctgacg aatggtcgct gaccagcttt gcgcattttg aacttctgca ccgtgcgaag
     2221 atgacgccca gtgtatcggc atcccgttgc gcagcgcgaa cagagaaaca tgcgattcgt
     2281 tgagaacaga ttcgatatta cgacaaatga tttcccccat ttcatgaaat ggtgggctgc
     2341 tgcacatggc ggcgagaata ttgccttcaa gctggcggat ttgcctttct tcggtgatat
     2401 ccgagaatgt cattaccagg ttctgcagat gcgcgagcac gtcataaacc gggctgatag
     2461 aggctttaat ccagattttt tcaccggtgc gcgtcaacag cagaaattcg tcctgatcgc
     2521 gggcggtttt ccatagcaac tgctgtaaac gaatacggtt atcagcaggg aattctggaa
     2581 tgttcaggag tgtatcgggc tgcataccgc tggcttcgtt aatgcagtaa ccaaacattt
     2641 cggtaaatgc gcgattgcac tgcacaatat ggcgttccgg atcgaggaca atcaccggtc
     2701 ggtcgagatg gtcaacggca ataatcaatt gtcgggtctg ttctttttgc gccatttcta
     2761 cgctggcatc ccgtaccagc gccaggtaat aaactttccc ctcggcgctc actttcgata
     2821 gcgcaaaacg ggtccagatt ttactgccgt cttttttctc cagctgcagc tcccgactca
     2881 tcccctcgac gcgcgcttta ccaccttcac ggttgtgacg aatgtattga ggatgcgcag
     2941 gacgcaaatc ccgcggaatc agcatatcaa tgttattgcc aatgacttct tcacgtttgt
     3001 acccccagag cttctctgcg gcggggttga aaaacatcac ttcatcattt tcgttaatta
     3061 acaccgcgcc catcatattt tgctcaaggg cggggaaaaa aatgccatcg gcggcattat
     3121 ccgcatcggt tagcttcatg attacctctg catcctggcg catctaaaga ctggctttac
     3181 agagttcaac acggtttcta cctcgtcttt tgcgatatac agagcttcat cggctatttg
     3241 aatgaggcgc tcatagtcag gatgaccatt aaacatggcg gcaccgatgg aaagtgagag
     3301 ggcaatatcc tcgccgtttg cggctttaaa ataatgtcga cctttatggt taaaccgcag
     3361 gccaaaatca accaaacaca acgtgcggga ttggctaact gcaccatatt ctcctgaaat
     3421 atgaagatat actgaaaaga aataagcgac ttaggacagt ttcaatctac gctactgttc
     3481 ttcagaagag tatagcccat cgtaattatt tttcggtgac agcgaatatc gtatgttttt
     3541 tcatattcat acatttttat tagggggatt atggctgttt aactaagtgt ggttaattgc
     3601 gtggttaatt tgacgtattt aagcatgatt gctagtggga tagttcaaga gggtaacaag
     3661 ccagtgggta aagcaccggc ttgttacaaa gtaagaatgg gagtttaact gccccaacga
     3721 ctttgcagat agctgaccgc ttgttgagtc tgcggtttat tcagatagtc ctcacggaac
     3781 aagattgtgc cgctaatttc cggcacagca tcgttaagat cgagctgctt tttcagttcc
     3841 ggtacgccgc cgttaatcat ccagtctggc tctatctttg acggttctcc tactttatag
     3901 aaggcgatac cgatatacag gcgggtcctg gtcggtttaa cgacatccgc ccaccatttt
     3961 gccaacacgt cataacgcgc ggcactccgt gagaatggcc agtaaatttg gggagcaatg
     4021 taatccagca acccttgttc cacccatcga cgggtatcag cgtaggattc gtcataggct
     4081 gccgcgcctc gggtatcgga accgagcgga tcgtgtgatc ggttacgcca cacgcctgcc
     4141 gggctaacgc caaattcgac ttccggctta atgcttttaa tggtgtgcga aaccttcgca
     4201 attaactgct gggtattgtt gcgccgccag tctgcttttg acgcaaatgc gcctccgtat
     4261 ttgcggtacg tttcgttatc atttagccgt gaacctggtg actcagtata gaaatagtca
     4321 tcaaactgca cgccatctac cggatagcgg gaaaccactt ccgcaactat tgatgtgatc
     4381 cagtcctgaa cctcagggat gcccgggtcg aggacaaagc gatcgccgga cgttctgatc
     4441 cagtcgcggt gttgcacata gacgctggcc ggttgttgag acagggtgct attcagttcc
     4501 ctgatagtac cgggcttcgt attaaccgat acgcgatagg ggttaaacca ggcgtgtact
     4561 ttcatcccac gcttgtgggc ttcgtcgagc ataaattgca gcggatcgta acccggattt
     4621 tcaccaatct taccggtcat aagatcggac cacggcaaaa ttttcgatgg ccacagggcg
     4681 gtaccatccg gcttgacctg aaaaaagacc gtgtttatgc cgagacgttg cagatgatcc
     4741 agtttgtcga tcatcgcctg ttgttgtaca cgtgcccggc tggtggggtt actattgtta
     4801 accgaggaaa ccggtggcca gtcgaggcga gacaccgtgg ccagccagat gccacgcatc
     4861 ggttgtgacg attgttgcgt cgtggctggt ggctttgagc ccgctggtgg tgtcaccatg
     4921 gactctggag gcgtgctttt acaactacac agtaaaagtg ccagtgcaac cagtatcgct
     4981 ggtcttctaa tcgctaattt ctcgtttcgg gagcagatat ccatatgtgc tggtttccgg
     5041 taaacagatg tgcgctcatt ctcgtactta tcccggttaa gtcaatacga caacaagcac
     5101 gaaaaaggga gcgatgaatt atcgctccct tgtcttataa gcattcagac atggttagtg
     5161 tttcttgtca ttcatcacaa tatagtgtgg tgaacgtgca cgcgggtgca ggaagaagtg
     5221 accttttggt gcgttctgac tgttgattgg ctccagagtg acgccggtat tcgctttgct
     5281 tttacgatca tgaacagcat agagaataaa gggcagggca agtaccacca ggaaactaac
     5341 gaccagtaat tcaacataca tatcggtaga gtcaccctgg atgttatccg gcggcaggaa
     5401 ggaaacaata aacgccataa ttgaagtcag cagaccgaca atagccacga ccagtttcac
     5461 ccctttacca ccagggatat taaatgtgcg ttttaagtca ggatgtttaa gcaccaacac
     5521 aatgtagcca ataaacagca tgaaataagc acacagataa atcaccaccg tcagcgccag
     5581 tgcgatcagg aaggacatgt tgttaccgcc accggtattg gtgaggatga tcaacgcgat
     5641 agaagtaatc accaactgcg aaatgaccag cgttaccggt acgccatttt tgttcatttt
     5701 agcgaatgcc gctggcagca ggtttttctg cgctgtgaca tacatcccgc gagaaggacc
     5761 aacaatccag gaagcgattt ccgccagaac acccagcaac agcagtgcgg agatcacgcg
     5821 aaccgtccac tcaatttccg gtgccacatg tgacatcaga acggtaaagg tttgcattac
     5881 ccctgcggag aggttgattt cattacccgg aatgaccatc gcaatagaca aaccgccaac
     5941 agagcttagg cagattgccg ccaccatcag cagtaacata gccagtggat agtcgcgccc
     6001 ggggttgctc atttcattga cgtgggttgc ggaagcttca acgcccatat aactcaaaat
     6061 gaaggcaaca aatacaacca gggtgcccac tttagagaag tcagggaaga aggtcttcgc
     6121 atccatttcg atagcaacgg gggcaccgga gtgcagataa atagccgcta atgcgatcaa
     6181 aataaatgca ggtaacagga taccggcgaa gaagccaact ttagcaattc gcgccgtgta
     6241 tttcgtgcca ccaaactgcg ttaatgccag cgcccaaaga atgatgagtg ctgcaatagt
     6301 tttggtaatg gggtcttcat tcagcgctgg ccatttcagg atgtaggaga gtgcccctaa
     6361 cacgaaatag agcatcggaa taaaaccaat ggcgatttgc agatagccaa atgagatcgc
     6421 tgcaaatccc catctcggcc ccagagtatt tgatacccag gcgaagacgc caccttcttc
     6481 ccagccgtcg acggtagcca tttccgcagc acaaagtccc acgggaataa accataaaat
     6541 cccgcctaat agcaggaaga agactaatga aaagcccgat gttgcgaagg tagggtattc
     6601 ataaacagcc attaccatcg atgccgttat ggcaaaaaat ccgagtaatg tgagctgctt
     6661 agctttacct gtctgtactg atgtagccat attatccccc taaaacggta ttcctgtcgg
     6721 aaccgcacct gttttgttct gaagcgtatt cagaacaata ttttccgttg ctaatgccag
     6781 tgaacagact ttggaaattg tcccgaaacg gcttcgtttc gggacaccgt taccgttaaa
     6841 cgttatcagg tgtgtttaaa gctgttctgc tgggcaatac cctgcagttt cgggtggtcg
     6901 ctgagatatt tcagggaggc tttgtagtct tccagcaaca gttcagcaaa gtccatttcg
     6961 aagccgcgac gacacataat gcgcatcacc acgatgtcgg tggcttcacc gccgagagtg
     7021 aaggccggaa cctgccagcc gcgcagacgc agacgttcag agaggtcgta cagggtgtat
     7081 cctggatctt caccatcttt cagtttgaag caaaccgccg ggatgccttc gtccgggcga
     7141 ccggtacaga tgaactcata cggccccagt ttggcgattt catccgccag ataagcagca
     7201 acctggtagg aagcgttctg tactttggta tagccttcac gaccgaggcg caggaattca
     7261 taatactgtg caatcacctg acccgccggg cgggagaagt tgatggcgaa agtcccaatc
     7321 tgaccaccga ggtagtcaac gttgaacacc agttcctgcg gcagcgcttc ttcatcacgc
     7381 cagataaccc agccgcagcc cagcggagcc agaccgaatt tatggcctga agcactgatc
     7441 gatttcacac gcggcaggcg gaagtcccag acgatatccg gggc
//
LOCUS       DAHHTG010000178         7367 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.181, whole genome shotgun sequence.
ACCESSION   DAHHTG010000178 DAHHTG010000000
VERSION     DAHHTG010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7367)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7367)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7367
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.181"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            559..1548
                     /gene="araF"
                     /locus_tag="NBI74_004142"
     CDS             559..1548
                     /gene="araF"
                     /locus_tag="NBI74_004142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter substrate-binding
                     protein AraF"
                     /protein_id="HCD1934389.1"
                     /translation="MHKFTKALAAIGLAAVMSQSAMAENLKLGFLVKQPEEPWFQTEW
                     KFADKAGKDLGFEVIKIAVPDGEKTLNAIDSLAASGAKGFVICTPDPKLGSAIVAKAR
                     GYDMKVIAVDDQFVNAKGKPMDTVPLVMMAATKIGERQGQELYKEMQKRGWDVKESAV
                     MAITSNELDTARRRTTGSMDALKAAGFPEKQIYQVPTKSNDIPGAFDAANSMLVQHPE
                     VKHWLIVGMNDSTVLGGVRATEGQGFKAADIIGIGINGVDAVSELSKAQATGFYGSLL
                     PSPDVHGYKSSEMLYNWVAKGVEPTKFTEVTDVVLITRDNFKEELEKKGLGGK"
     gene            1618..3132
                     /gene="araG"
                     /locus_tag="NBI74_004143"
     CDS             1618..3132
                     /gene="araG"
                     /locus_tag="NBI74_004143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter ATP binding protein
                     AraG"
                     /protein_id="HCD1934390.1"
                     /translation="MQQSTPYLSFRGIGKTFPGVKALTDISFDCYAGQVHALMGENGA
                     GKSTLLKILSGNYAPTTGSVVINGQEMSFSDTTAALNAGVAIIYQELHLVPEMTVAEN
                     IYLGQLPHKGGIVNRSLLNYEAGLQIKHLGMDIDPDTPLKYLSIGQWQMVEIAKALAR
                     NAKIIAFDEPTSSLSAREIDNLFRVIRELRKEGRVILYVSHRMEEIFALSDAITVFKD
                     GRYVKTFTDMQQVDHDALVQAMVGRDIGDIYGWQPRSYGEERLRLDAVKAPGVRTPIS
                     LAVRSGEIVGLFGLVGAGRSELMKGMFGGTQITAGQVYIDQQPIDIRKPSHAIAAGMM
                     LCPEDRKAEGIIPVHSVRDNINISARRKHVLGGCVINNGWEENNADHHIRSLNIKTPG
                     AEQLIMNLSGGNQQKAILGRWLSEEMKVILLDEPTRGIDVGAKHEIYNVIYALAAQGV
                     AVLFASSDLPEVLGVADRIVVMREGEIAGELLHEQADERQALSLAMPKVSQAVA"
     gene            3147..4133
                     /gene="araH"
                     /locus_tag="NBI74_004144"
     CDS             3147..4133
                     /gene="araH"
                     /locus_tag="NBI74_004144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026162.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter permease AraH"
                     /protein_id="HCD1934391.1"
                     /translation="MSSVSTSGSGAPKSSFSFGRIWDQYGMLVVFAVLFIACAIFVPN
                     FATFINMKGLGLAISMSGMVACGMLFCLASGDFDLSVASVIACAGVTTAVVINLTESL
                     WIGVAAGLLLGILCGLVNGFVIAKLKINALITTLATMQIVRGLAYIISDGKAVGIEDE
                     SFFALGYANWFGLPAPIWLTVACLIIFGLLLNKTTFGRNTLAIGGNEEAARLAGVPVV
                     RTKIIIFVLSGLVSAIAGIILASRMTSGQPMTSIGYELIVISACVLGGVSLKGGIGKI
                     SYVVAGILILGTVENAMNLLNISPFAQYVVRGLILLAAVIFDRYKQKAKRTV"
     gene            4300..5100
                     /gene="otsB"
                     /locus_tag="NBI74_004145"
     CDS             4300..5100
                     /gene="otsB"
                     /locus_tag="NBI74_004145"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HCD1934392.1"
                     /translation="MTEPLTETPELSAKYAWFFDLDGTLAEIKPHPDQVVVPDTILQG
                     LQLLATASDGALALISGRSMVELDALAKPYRFPLAGVHGAERRDINGKTHIVHLPDAI
                     ARDISVQLHTVIAQYPGAELEAKGMAFALHYRQAPQHEDALMTLAQRITQIWPQMALQ
                     QGKCVVEIKPKGTSKGEAITAFMQEAPFIGRTPVFLGDDLTDESGFAVVNRLGGMSVK
                     IGTGATQASWRLAGVPDVWSWLEMITTALQQKRENNRSDDYESFSRSI"
     gene            5075..6499
                     /gene="otsA"
                     /locus_tag="NBI74_004146"
     CDS             5075..6499
                     /gene="otsA"
                     /locus_tag="NBI74_004146"
                     /EC_number="2.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalose-phosphate synthase"
                     /protein_id="HCD1934393.1"
                     /translation="MSRLVVVSNRIAPPDEHAASAGGLAVGILGALKAAGGLWFGWSG
                     ETGNEDQPLKKVKKGNITWASFNLSEQDLDEYYNKFSNAVLWPAFHYRLDLVQFQRPA
                     WDGYLRVNALLADKLLPLLQDDDIIWIHDYHLLPFAHELRKRGVNNRIGFFLHIPFPT
                     PEIFNALPTYDTLLEQLCDYDLLGFQTENDRLAFLDCLSNLTRVTTRSAKNHTACGKA
                     FRTEVYPIGIEPKEIAKQAAGPLPPKLAQLKAELKNVQNIFSVERLDYSKGLPERFLA
                     YEALLEKYPQHHGKIRYTQIAPTSRGDVQAYQDIRHQLENEAGRINGKYGQLGWTPLY
                     YLNQHFDRKLLMKIFRYSDVGLVTPLRDGMNLVAKEYVAAQDPANPGVLVLSQFAGAA
                     NELTSALIVNPYDRDEVAAALDRALTMSLAERISRHAEMLDVIVKNDINHWQECFISD
                     LKQIVPRSAESQQRDKVATFPKLA"
     gene            complement(6506..6934)
                     /gene="uspC"
                     /locus_tag="NBI74_004147"
     CDS             complement(6506..6934)
                     /gene="uspC"
                     /locus_tag="NBI74_004147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000122413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspC"
                     /protein_id="HCD1934394.1"
                     /translation="MSYSNILVAVAVTPESQQLLAKAVSIARPVKGHISLITLASDPE
                     MYNQLAAPMLEDLRNVMQEETQSFLDKLIQDAGYPVDKTFIAYGELSEHILEVCRKHH
                     FDLVICGNHNHSFFSRASCSAKRVITSSEVDVLLVPLTGG"
BASE COUNT         1855 a         1683 c         1891 g         1938 t
ORIGIN      
        1 ggtgcctttt gtatcttgtt gatattaatc gatgaatttg aattcatgat gaagcaatga
       61 ttttataaaa cgttttttca tttttgcgat atggctctca ttatacgtgt tctgttaatt
      121 aatgagcacg gtgataatta atggcgaaat gaatgacgcg cattttccac atctttaagt
      181 tgcggttatt acaccattgc aaaaactcaa cgccaggtaa tgaggcctat tgactggtta
      241 aaaagaagac atcccgcatg ggtaccaaag acaacaagga cttccaggct aatcttatgg
      301 attattctgc tgtgcactcg ataatttgtc tgacaaattc gcttttcctt atgtcttttc
      361 ccgctaaatt tatgcacgtt ctcactgtaa ttctgcgatg tgatattgct ctcctatgga
      421 gaattaattt ctcgctaaaa ctatgtcaac acagtcactt atcttttagt taaaaggtaa
      481 tgctttgttt tccgattaat ttaacgaatg tcattcgttt ttgccctaca caaaacgaca
      541 ctaaagctgg agagaaccat gcacaaattt actaaagccc tggcagccat tggtctggca
      601 gccgttatgt cacaatccgc tatggcggag aacctgaagc tcggtttcct ggtgaagcaa
      661 ccggaagagc cgtggttcca gaccgaatgg aagtttgccg ataaagccgg gaaggattta
      721 gggtttgaag ttattaagat tgccgtgccg gatggcgaaa aaacattgaa cgctatcgac
      781 agcctggctg ccagtggcgc gaaaggtttc gttatttgta ctccggaccc gaaactcggc
      841 tctgccatcg tggcgaaagc gcgtggctac gatatgaaag tcattgccgt ggatgaccag
      901 tttgttaacg ccaaaggtaa gccaatggat accgttccgc tggtgatgat ggcagcgacc
      961 aaaattggcg aacgtcaggg ccaggaactg tataaagaga tgcagaaacg tggctgggat
     1021 gtcaaagaaa gtgcggtgat ggcgattacc tccaacgaac tggatacggc tcgtcgccgt
     1081 actacggggt ctatggatgc gctgaaagct gcaggattcc cggaaaaaca aatttatcag
     1141 gtacctacca aatctaacga catcccgggg gcatttgacg ctgccaactc aatgctggtt
     1201 caacatccgg aagttaaaca ttggctgatt gtcggtatga acgacagcac cgtgctgggc
     1261 ggcgtacgcg cgacggaagg tcagggcttt aaagcggccg atatcatcgg tatcggtatt
     1321 aacggtgtgg atgcagtgag tgaactgtct aaggcgcagg caaccggctt ctacggttcc
     1381 ctgctgccaa gcccggacgt acatggttat aagtccagcg aaatgcttta caactgggtt
     1441 gcaaaaggtg ttgaaccgac caaattcacc gaagttaccg acgtggtgct gatcacgcgt
     1501 gacaacttta aagaagaact ggagaaaaaa ggtttaggcg gtaagtaatt tgccggaaaa
     1561 attcccctct gcatgatgca gagggggtgt gaacgaccag tgattcacgg agacgttatg
     1621 caacagtcta ccccgtatct ctcatttcgc ggcatcggta aaacgtttcc cggcgttaag
     1681 gcgctgacgg atattagttt tgactgctat gccggtcagg ttcatgcgtt gatgggtgaa
     1741 aatggcgcag gaaaatcaac tctcttaaaa atcctcagcg gaaactatgc gccaaccacg
     1801 ggttctgtag tgattaatgg gcaggaaatg tccttttccg acacgaccgc agcacttaac
     1861 gcgggtgtgg cgattattta tcaggaactg catctcgtgc cggaaatgac cgtcgcggaa
     1921 aacatctatc tcggccagct gccgcataaa ggcggcattg tgaatcgttc attgctgaat
     1981 tatgaggcag gtttacaaat taaacatctt ggtatggata ttgacccgga cacgccgctg
     2041 aaatatctct ccattggtca gtggcagatg gttgaaatcg ccaaagcgct ggcacgtaac
     2101 gccaaaatta tcgcctttga tgagccaacc agctccctct ctgcccgcga aatcgacaat
     2161 cttttccgcg ttattcgtga actgcgaaaa gaggggcggg tgatcttata cgtttctcac
     2221 cgtatggaag aaatatttgc cctcagcgat gccatcaccg tctttaaaga tgggcgttat
     2281 gtcaaaacct ttaccgatat gcagcaggtt gaccacgacg cactggtgca ggcgatggtc
     2341 gggcgcgaca ttggcgatat ctacggctgg caaccgcgta gttatggcga ggagcggctg
     2401 cgtcttgatg ctgtgaaagc accaggcgtg cgtacgccaa taagtctggc ggttcgcagt
     2461 ggtgaaattg ttgggctgtt tggtctggtc ggggcagggc gtagcgaatt aatgaaaggc
     2521 atgtttggcg ggacgcaaat caccgccggt caggtttata tcgaccaaca gccgatcgat
     2581 attcgtaaac cgagccacgc cattgccgca ggcatgatgc tctgcccgga agatcgcaaa
     2641 gcggaaggca ttattcccgt gcactccgtt cgcgacaata tcaacatcag tgccagacgt
     2701 aaacatgtgc ttggcggttg tgtaatcaac aacggttggg aagaaaacaa tgccgatcac
     2761 cacattcgtt cgctcaacat caaaacgccg ggcgctgagc aactgatcat gaatctctca
     2821 ggcggaaatc agcaaaaagc cattctgggc cgctggttat cggaagagat gaaggtcatt
     2881 ttgctggatg aacctacgcg tggcattgat gttggtgcta agcatgaaat ttacaacgtg
     2941 atttacgcgc tggcggcgca gggtgtggcg gtgctgtttg cctccagcga cttacctgaa
     3001 gtcctcggcg ttgccgaccg gattgtggtg atgcgggaag gcgaaatcgc cggtgaattg
     3061 ttacacgagc aggcagatga gcgtcaggca ctgagccttg cgatgcctaa agtcagccag
     3121 gcagttgcct gagtaaggag agtatgatgt cttctgtttc tacatcgggt tctggcgcac
     3181 ctaagtcgtc attcagcttc gggcgtatct gggatcagta cggcatgctg gtggtgtttg
     3241 cggtgctttt tatcgcctgt gccatttttg tcccaaattt tgccaccttc attaatatga
     3301 aagggttggg cctggcaatt tccatgtcgg ggatggtggc ttgtggcatg ttgttctgcc
     3361 ttgcttccgg tgactttgac ctttctgtcg cctccgtaat tgcctgtgcg ggtgtcacca
     3421 cggcggtggt tattaatctg actgaaagcc tgtggattgg cgtggcagcg gggttgctgc
     3481 tgggcattct ctgtggcctg gtcaatggct ttgttatcgc caaactcaaa ataaatgccc
     3541 tgatcacgac actggcaacg atgcagattg ttcgaggtct ggcgtacatc atttcagacg
     3601 gtaaagcggt cggtatcgaa gatgaaagct tctttgccct tggttacgcc aactggttcg
     3661 gtctgcctgc gccaatctgg ctcaccgtcg cgtgtttgat catctttggc ttgctgctga
     3721 ataaaaccac ctttggtcgt aataccctgg cgattggcgg gaacgaagag gctgcgcgtc
     3781 tggcgggtgt accggttgtt cgcaccaaaa ttattatctt tgttctctct ggcctggtat
     3841 cagcgatagc cggaattatt ctggcttcac gtatgaccag tgggcagcca atgacgtcga
     3901 ttggttatga gctgattgtg atctccgcct gcgttttagg tggcgtttct ctgaaaggcg
     3961 gcatcggaaa aatctcatat gtggtggcgg gtatcttaat tttaggcacc gtggagaacg
     4021 ccatgaacct gcttaatatt tctcctttcg cgcagtacgt ggttcgcggc ttaatcctgc
     4081 tggcagcggt gatcttcgac cgttacaagc aaaaagcgaa acgcactgtc tgatgctttt
     4141 ttctgcaaca atttagcgct tttttcctcc acagccagcc gccacaacgg ttggctgttc
     4201 ttcgttacaa atggcgaccc ccgtcacact gtctatactt acatgtctgt aaagcgcgtt
     4261 ctgcgcaaca caataagaaa agagaaggag gagaaccggg tgacagaacc gttaaccgaa
     4321 acccctgaac tatccgcgaa atatgcctgg ttttttgatc tcgatggaac gctggcggaa
     4381 atcaaaccgc atcccgatca ggtcgtcgtg cctgacacta ttctgcaagg acttcagcta
     4441 ctggcaaccg caagtgatgg tgcattggca ttgatttcag ggcgctcaat ggtggagctt
     4501 gacgcactgg caaaacctta tcgcttccca ttagcgggcg tgcatggggc ggagcgccgt
     4561 gacatcaatg gtaaaacgca tatcgttcat ctgccggatg cgattgcgcg tgatattagc
     4621 gtgcaactgc atacggtcat cgcccagtat cccggcgcgg agctggaggc gaaagggatg
     4681 gcttttgcgc tgcattatcg ccaggctccg cagcatgaag acgcattaat gacattagcg
     4741 caacgtatta ctcagatctg gccacaaatg gcgttacagc agggaaagtg tgttgtcgag
     4801 atcaaaccga aaggtaccag taaaggtgag gcgattactg cctttatgca ggaagctccc
     4861 tttatcgggc gaacgcccgt atttctgggc gatgatctaa ccgatgaatc tggcttcgca
     4921 gtcgttaacc ggctgggcgg aatgtcagta aaaattggca caggtgcaac tcaggcatca
     4981 tggcgactgg cgggcgtgcc ggatgtctgg agctggcttg aaatgataac caccgcatta
     5041 caacaaaaaa gagaaaataa caggagtgat gactatgagt cgtttagtcg tagtatctaa
     5101 ccggattgca ccaccagacg agcacgccgc cagtgccggt ggccttgccg ttggcatact
     5161 gggggcactg aaagccgcag gtggactgtg gtttggctgg agcggtgaaa cagggaatga
     5221 ggatcagccg ctaaaaaagg tgaaaaaggg taacattacg tgggcctctt ttaacctcag
     5281 cgaacaggac cttgacgaat actacaacaa attctccaat gccgttctct ggcccgcttt
     5341 tcattatcgg ctcgatctgg tgcaatttca gcgtcctgcc tgggacggtt atctacgcgt
     5401 aaatgcgttg ctggcagata aattactgcc gctgttgcaa gacgatgaca ttatctggat
     5461 ccacgattat cacctgttgc cctttgcgca tgaattacgc aaacggggcg tgaataatcg
     5521 cattggtttc tttctgcata ttcctttccc gacaccggaa atcttcaacg cgctgccgac
     5581 atatgacacc ttgcttgaac agctttgtga ttatgatttg ctgggtttcc agacggagaa
     5641 cgatcgtctg gcgttcctgg attgcctttc taacctgacc cgcgtcacga cacgtagcgc
     5701 aaaaaaccat acagcctgcg gcaaagcgtt tcgaacggaa gtttacccga tcggcattga
     5761 gccgaaagaa atagcgaaac aggctgccgg tccactgccg ccaaaactgg cgcaactcaa
     5821 agcggaactg aaaaacgtac aaaatatctt ttctgtcgaa cgactggatt attccaaagg
     5881 tttgccggag cgttttctcg cctatgaagc gttactggaa aaatatccgc aacatcacgg
     5941 taaaattcgt tatacccaga tcgcgccaac gtctcgtggt gatgtgcaag cctatcagga
     6001 tattcgtcat cagctcgaaa atgaagccgg acgaattaat ggtaaatatg ggcaattagg
     6061 ctggacgccg ctttattatt tgaatcagca ttttgaccgt aaattactga tgaaaatatt
     6121 ccgctactct gacgtgggct tagtgacgcc actgcgtgac gggatgaacc tggtggcaaa
     6181 agagtatgtt gccgctcagg acccagccaa cccgggcgtt cttgttcttt cgcaatttgc
     6241 gggagcggca aacgagttaa cgtcggcgtt aattgttaat ccctacgatc gtgacgaagt
     6301 tgcagctgcg ctggatcggg cattgactat gtcgctggcg gaacgtattt cccgtcatgc
     6361 agaaatgctg gacgttatcg tgaaaaacga tattaaccac tggcaggagt gcttcattag
     6421 cgacctaaag cagatagttc cgcgaagcgc ggaaagccag cagcgcgata aagttgctac
     6481 ctttcctaag cttgcgtagg tgcggttaac ctcccgtaag tggaactaac agcacatcga
     6541 cctcacttga ggtaatcact cttttcgccg agcaggatgc tcgcgaaaag aaactgtgat
     6601 tgtgattacc gcaaatcacc aaatcgaaat gatgtttgcg acaaacttcc agaatatgtt
     6661 cgcttaactc accgtaggca ataaacgtct tgtcaacggg ataccccgca tcctgaatta
     6721 acttatcaag aaagctttgc gtttcttcct gcatcacgtt gcgtaaatct tcaagcatcg
     6781 gcgcagctaa ttgattgtac atttccgggt cggaagcgag agtaattaaa ctgatgtgtc
     6841 cctttactgg cctggcgata gataccgctt ttgccagcag ttgctgactt tccggtgtaa
     6901 ccgcaacggc gacaagaata ttgctatagc tcataacctg ttccttattc tgtgaacttc
     6961 aggtgacatt aaagctttac tcctaatgga gaaacgacgc aatcccaact cagttaaaca
     7021 ggtaaatatt agtctggtca ttagtaaata caattaatca attactcttg gttatcatat
     7081 gatttcaact gtatttataa gaaaataatg tactgatttc ccatattgaa caagtggtta
     7141 atatatgtaa aacattttat taacattagg ttgattgttg cctttctttg tagttaatta
     7201 gtttgttgtg cggtaagtgt ttgtttaaaa atggcttatc tggtattgca ttaaagcgat
     7261 tatttatagc agatgattat ttacggtgag ttattttaac tgtgcgcaac atcccatttc
     7321 gattattcct gtttcatttt tgcttgccag tgtagcgaaa aattttt
//
LOCUS       DAHHTG010000179         7297 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.182, whole genome shotgun sequence.
ACCESSION   DAHHTG010000179 DAHHTG010000000
VERSION     DAHHTG010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7297)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7297)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7297
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.182"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            245..1777
                     /locus_tag="NBI74_004148"
     CDS             245..1777
                     /locus_tag="NBI74_004148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVR family protein"
                     /protein_id="HCD1934395.1"
                     /translation="MATIDSMNKDTTRLSDGPDWTFDLLDVYLAEIDRVAKLYRLDTY
                     PHQIEVITSEQMMDAYSSVGMPINYPHWSFGKKFIETERLYKHGQQGLAYEIVINSNP
                     CIAYLMEENTITMQALVMAHACYGHNSFFKNNYLFRSWTDASSIVDYLIFARKYITEC
                     EERYGVDEVERLLDSCHALMNYGVDRYKRPQKISLQEEKARQKSREEYLQSQVNMLWR
                     TLPKREEEKTVAEARRYPSEPQENLLYFMEKNAPLLESWQREILRIVRKVSQYFYPQK
                     QTQVMNEGWATFWHYTILNHLYDEGKVTERFMLEFLHSHTNVVFQPPYNSPWYSGINP
                     YALGFAMFQDIKRICQSPTEEDKYWFPDIAGSDWLETLHFAMRDFKDESFISQFLSPK
                     VMRDFRFFTVLDDDRHNYLEISAIHNEEGYREIRNRLSSQYNLSNLEPNIQIWNVDLR
                     GDRSLTLRYIPHNRAPLDRGRKEVLKHVHRLWGFDVMLEQQNEDGSIELLERCPPRMG
                     NL"
     gene            complement(1829..2548)
                     /gene="fadR"
                     /locus_tag="NBI74_004149"
     CDS             complement(1829..2548)
                     /gene="fadR"
                     /locus_tag="NBI74_004149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid metabolism transcriptional regulator
                     FadR"
                     /protein_id="HCD1934396.1"
                     /translation="MVIKAQSPAGFAEEYIIESIWNNRFPPGTILPAERELSELIGVT
                     RTTLREVLQRLARDGWLTIQHGKPTKVNNFWETSGLNILETLARLDHESVPQLIDNLL
                     SVRTNISTIFIRTAFRQHPDKAQEVLATANEVADHADAFAELDYNIFRGLAFASGNPI
                     YGLILNGMKGLYTRIGRHYFANPEARSLALGFYHKLSALCSEGAHDQVYETVRRYGHE
                     SGEIWHRMQKNLPGDLAIQGR"
     gene            2770..4311
                     /gene="nhaB"
                     /locus_tag="NBI74_004150"
     CDS             2770..4311
                     /gene="nhaB"
                     /locus_tag="NBI74_004150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)/H(+) antiporter NhaB"
                     /protein_id="HCD1934397.1"
                     /translation="MEISWGRALWRNFLGQSPDWYKLALIIFLIVNPLIFLISPFVAG
                     WLLVAEFIFTLAMALKCYPLLPGGLLAIEAVFIGMTSAEHVREEVAANLEVLLLLMFM
                     VAGIYFMKQLLLFIFTRLLLSIRSKMLLSLSFCVAAALLSAFLDALTVVAVVISVAVG
                     FYGIYHRVASSRTEDTDLQDDSHIDKHYKVVLEQFRGFLRSLMMHAGVGTALGGVMTM
                     VGEPQNLIIAKAAGWHFGDFFLRMSPVTVPVLICGLLTCLLVEKLRWFGYGETLPEKV
                     REVLQQFDDQSRNQRTRQDKIRLIVQAIIGVWLVTALALHLAEVGLIGLSVIILATSL
                     TGVTDEHAIGKAFTESLPFTALLTVFFSVVAVIIDQQLFSPIIQFVLQASEHAQLSLF
                     YIFNGLLSSISDNVFVGTIYINEAKAAMESGAITLKQYELLAVAINTGTNLPSVATPN
                     GQAAFLFLLTSALAPLIRLSYGRMVWMALPYTLVLTVAGLLCVEFTLAPVTEWFMQMG
                     WIATL"
     gene            4457..4987
                     /gene="dsbB"
                     /locus_tag="NBI74_004151"
     CDS             4457..4987
                     /gene="dsbB"
                     /locus_tag="NBI74_004151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein DsbB"
                     /protein_id="HCD1934398.1"
                     /translation="MLRFLNQCSQGRGAWLLMAFTALALELTALWFQHVMLLKPCVLC
                     IYERCALFGVLGAALIGAIAPKTPLRYVAMVIWLYSAFRGVQLTYEHTMLQLYPSPFA
                     TCDFMARFPEWLPLDKWVPQVFVASGDCAERQWEFLGLEMPQWLLGIFIAYLIVAVLV
                     VISQPFKAKKRDLFGR"
     gene            complement(5032..6300)
                     /gene="umuC"
                     /locus_tag="NBI74_004152"
     CDS             complement(5032..6300)
                     /gene="umuC"
                     /locus_tag="NBI74_004152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase V catalytic protein"
                     /protein_id="HCD1934399.1"
                     /translation="MFALCDVNAFYASCETVFRPDLWGKPVVVLSNNDGCVIARNAEA
                     KALGVKMGDPWFKQKDLFRRCGVVCFSSNYELYADMSNRVMSTLEELSPRVEIYSIDE
                     AFCDLTGVHNCRDLTDFGREIRATVLQRTHLTVGVGIAQTKTLAKLANHAAKKWQRQT
                     GGVVDLSNLERQRKLMSALPVDEVWGIGRRISKKLDAMGIKTVLDLADSDIRFIRKHF
                     NVVLERTVRELRGEPCLQLEEFAPTKQEIICSRSFGERITDYPSMRQAICSYAARAAE
                     KLRSEHQYCRFISTFIKTSPFAFNEPYYGNSASVKLLTPTQDSRDIINAATRSLDAIW
                     QAGHRYQKAGVMLGDFFSQGVAQLNLFDDNAPRPGSEQLMAVMDTLNAKEGRGTLYFA
                     GQGIQQQWQMKRAMLSPCYTTRSSDLLRVK"
     gene            complement(6300..6719)
                     /locus_tag="NBI74_004153"
     CDS             complement(6300..6719)
                     /locus_tag="NBI74_004153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HCD1934400.1"
                     /translation="MLFIKPADLREIVTFPLFSDLVQCGFPSPAADYVEQRIDLNQLL
                     IQHPSATYFVKASGDSMIDGGISDGDLLIVDSAITASHGDIIIAAVDGEFTVKKLQLR
                     PTVQLIPMNSAYSPITISSEDTLDVFGVVIHVVKAMR"
     gene            6916..>7297
                     /locus_tag="NBI74_004154"
     CDS             6916..>7297
                     /locus_tag="NBI74_004154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin E"
                     /protein_id="HCD1934401.1"
                     /translation="MEAFAQKGILIKVLDDGIKKLCSIAAGVVEGKLIPELKNKLKSV
                     QSFFTTLSNTVKQANKDIDAAKLKLTTEIAAIGEIKTETETARFYVDYDDLMLSLLKE
                     AAQKMINTCNEYQKRHGKKTLFEIP"
BASE COUNT         1786 a         1815 c         1786 g         1910 t
ORIGIN      
        1 cggctttttt atggtgagct ggctcacatc tccctgactc tgcggcaata tggcgtcttt
       61 agtatctcct gctgaacgcc ctttttatcc agcataaaat tccgttcaga agcggattag
      121 tggcactctg ataataagaa atcgcaaaga aaaaaattct ccggcgacgc ggtttttaac
      181 aggtgttcta tgcttgaaat gaggtgctcc acacagagag cgccgacaac aatgaggtgc
      241 gcgtatggcg acgatcgatt ctatgaataa ggacaccaca cgtttgagcg atggacccga
      301 ctggacgttc gacctgctgg atgtttatct ggcagagata gaccgggtgg cgaaactcta
      361 ccggctggat acctacccgc accagattga agtgataacc tcagaacaga tgatggatgc
      421 ttactccagc gtcggcatgc caattaacta tccgcactgg tcattcggta aaaagtttat
      481 cgagactgaa cggctgtata agcacggtca gcaaggactg gcctatgaaa tcgtcattaa
      541 ctctaacccg tgtatcgctt acctgatgga agagaacacc attaccatgc aagcgttggt
      601 gatggctcat gcctgctatg ggcataactc tttcttcaaa aacaattact tattccgtag
      661 ctggaccgac gccagttcga ttgtcgatta tctgattttt gcccgtaaat atattaccga
      721 gtgcgaagaa cgttatggcg ttgatgaagt agaacggctt ctggactcgt gccacgcgct
      781 gatgaactac ggcgtggacc ggtacaaacg cccgcaaaaa atctcgctgc aagaagagaa
      841 agcccggcag aagagtcgcg aagagtatct gcaaagtcag gtcaatatgc tctggcgtac
      901 cctgccgaag cgcgaggaag agaaaacggt tgctgaagcg cgccgctatc cgtccgaacc
      961 acaagaaaac ctgctctatt ttatggagaa aaatgcgcca ctgctggaat catggcagcg
     1021 tgaaatcctg cgtattgtgc gtaaggtgag ccagtatttt tatccgcaaa aacagactca
     1081 ggtgatgaac gaaggctggg cgaccttctg gcactacacc atccttaacc atctgtatga
     1141 tgaagggaaa gtaacggaac gttttatgct ggagtttttg cacagccaca ccaatgtggt
     1201 cttccagccg ccctataaca gcccgtggta cagcggcatc aacccatatg ccctcgggtt
     1261 cgccatgttc caggatatta aacggatttg tcagtcgccg acggaagaag acaaatactg
     1321 gttcccggat atcgccggtt ccgactggct ggaaacgctg catttcgcga tgcgtgattt
     1381 caaagatgag agttttatca gccagttcct gtcaccgaaa gtgatgcgtg atttccgctt
     1441 cttcaccgtg ctggatgatg atcgacataa ttatctggag atttccgcta ttcataatga
     1501 agaaggttat cgggaaatcc gtaaccggtt atcgtcgcaa tataacttaa gtaatctgga
     1561 gccgaatatt cagatctgga acgtggattt gcgcggtgac cgttcgctga cgctgcgtta
     1621 cattccacat aatcgcgcac cgctggatcg ggggcgcaaa gaagtgctga agcatgtgca
     1681 tcgcctgtgg ggatttgatg tgatgctgga acagcaaaac gaagacggca gcatcgagtt
     1741 gctggaacgt tgcccgccga gaatgggaaa tctgtaaaaa caacgaaaaa cccctcgttt
     1801 gaggggtttg ctttttaaac ggaagggatt atcgtccctg aatggctaaa tcacccggca
     1861 gatttttctg catccggtgc caaatctcgc cactctcatg cccatagcga cgcactgttt
     1921 catatacctg atcgtgtgcg ccttcactgc acaacgccga cagtttgtga tagaagccca
     1981 gcgccagact gcgcgcttcc ggattggcga aatagtgacg accaatgcgc gtatacagcc
     2041 ctttcatccc gttaaggatc agaccgtaaa tcgggttgcc ggaagcaaac gccaggccgc
     2101 ggaaaatgtt gtaatccagc tcggcaaagg cgtcggcgtg atcggccact tcattagcgg
     2161 ttgccagcac ttcctgcgct ttatcgggat gttgacgaaa cgcggtgcga ataaaaatag
     2221 ttgaaatatt ggtacgcacc gacagcaaat tatcaataag ctgcggcaca ctttcgtggt
     2281 ccagtcgcgc cagtgtttca aggatattta aaccagaggt ttcccagaaa ttattcacct
     2341 tcgtcggctt gccgtgttga atggtcaacc agccatctcg tgccagacgt tgtaacactt
     2401 cacgtaacgt agtacgcgtt acgccaatta attctgaaag ttcacgttct gcgggcaaaa
     2461 tagtcccggg agggaagcgg ttattccaga tactttcaat aatgtactct tccgcgaaac
     2521 ccgccgggct ttgcgcctta atgaccatag tgagatttcc ataacacagc aaaacaaagt
     2581 tggactcatc ataccagagg gctaactacg ctgatagaag acaaaaagaa gaaaaaaggg
     2641 atcaaggctt cattttctgc atctctttcc ccttgactat caagacttgc ctcactcctg
     2701 tctgaccgtt aagctttgcg tttaaacata aaaacatcat tgattatttc taaggtaagg
     2761 gaaaccatca tggagatctc ctggggccgc gcactatggc gcaatttttt gggccagtcc
     2821 cccgactggt acaaactcgc cctcatcatt ttcttaatcg taaatccgtt aattttcctc
     2881 atcagccctt tcgtcgctgg ctggttgctg gtagcggaat ttattttcac tctggcgatg
     2941 gccctgaaat gctacccgct gctccccggt ggtctgttgg ctatcgaagc ggtattcatc
     3001 ggcatgacca gcgcggaaca tgttcgtgaa gaagtggcgg caaatcttga agtcttgctg
     3061 ttactgatgt ttatggtggc gggtatctat tttatgaaac agctgttgtt gttcatattt
     3121 acccgtttgc tgttaagcat tcgctccaaa atgctgctgt cgctctcttt ttgcgtggcg
     3181 gcggcgctcc tctccgcgtt cctcgatgcc ttaaccgtcg tggcggtggt gatcagcgtt
     3241 gcagtcggtt tttatggtat ttatcaccgc gtagcctctt cccgtactga agataccgac
     3301 ctgcaagacg atagtcatat cgacaagcat tacaaagtgg ttctggaaca gttccgtggc
     3361 tttctgcgta gtctgatgat gcatgccggt gtcggcaccg cattaggcgg cgtgatgacc
     3421 atggtgggcg aaccacagaa cctgatcatc gctaaagcgg ctggctggca ttttggcgat
     3481 ttcttcctgc gcatgtcgcc ggtgaccgtc ccggttctga tttgtggcct gttaacctgc
     3541 ctgctggtag agaagctgcg ttggtttggc tacggtgaaa cgctgccgga gaaagtccgc
     3601 gaagtactgc aacagtttga cgatcaaagc cgcaaccagc gtacccgtca ggataaaatc
     3661 cgtctgattg tccaggcgat tatcggcgtc tggctggtga ctgcgctggc actgcatctg
     3721 gcggaagttg gtttgattgg tttgtcagtc attattctgg cgacatcatt gaccggtgtt
     3781 accgatgaac atgctatcgg taaagccttc accgaatctc tgccattcac cgcactgttg
     3841 acggtgtttt tctcagtagt cgcggtgatt atcgaccaac aactgttttc gccgattatt
     3901 cagtttgtgt tgcaggcatc tgaacatgct cagctgtcgt tgttctatat tttcaacggt
     3961 ctgctgtcat ccatttcgga taacgtcttc gtggggacga tttatatcaa cgaagcgaaa
     4021 gcggcaatgg aaagtggcgc tatcacgttg aagcaatatg agctgctggc ggtcgccatt
     4081 aataccggta ccaatctgcc ctccgtcgct acgccgaacg gtcaggctgc gttcctgttc
     4141 ctgctgacct ctgcactcgc gccattgatt cgtctctctt atggccgcat ggtgtggatg
     4201 gccctgcctt acaccctcgt actgacggtt gccgggttac tctgcgtcga gtttacgctt
     4261 gctcctgtaa ccgaatggtt tatgcaaatg ggctggatag caacgctttg ataacaactt
     4321 accgggcata taaatgcccg gtttgccttt tcgcccgata attgtccaat tgcgtcctat
     4381 ttttgctgcc tcctggtggc gcagcgaatg aattggttta aactgcgcac tctatgcata
     4441 ttgcagggaa atgattatgt tgcgattttt gaaccaatgt tcacaaggcc ggggcgcatg
     4501 gctgttgatg gcgtttactg ctctggcact ggaactgacg gcgctgtggt tccagcatgt
     4561 gatgttactg aaaccttgcg tgctctgtat ttatgaacgc tgcgcgttat tcggcgttct
     4621 gggtgctgcg ctgattggcg cgatcgcccc gaaaactccg ctgcgttatg tagcgatggt
     4681 tatctggttg tatagtgcgt tccgcggtgt gcagttaact tacgagcaca ccatgcttca
     4741 gctctatcct tcgccgtttg cgacctgtga ttttatggct cgtttcccgg aatggctgcc
     4801 gctggataaa tgggtgccgc aagtgtttgt cgcctctggc gattgcgccg agcgtcagtg
     4861 ggaattttta ggtctggaaa tgccgcagtg gctgctcggt atttttatcg cttacctgat
     4921 tgtcgcggtg ctggtggtga tttcccagcc gtttaaagcg aaaaaacgtg acctgttcgg
     4981 tcgctaatcc attcggcgct cctgcgggag cgctttttcc tgccgctatg tttatttgac
     5041 ccgcagtaaa tcagaacttc gcgttgtata acatggtgaa agcatggcac gcttcatctg
     5101 ccattgttgc tggatcccct gcccggcaaa atagagtgtt cctctgccct ctttagcatt
     5161 cagcgtatcc attaccgcca tcaattgctc actcccgggg cgtggtgcgt tgtcatcgaa
     5221 taaattgagc tgcgcgactc cctgactgaa gaaatccccc agcatcacgc ccgctttttg
     5281 gtagcgatgg cccgcttgcc agatggcatc cagagatcgc gtagcggcgt taatgatatc
     5341 cctgctgtcc tgagtgggcg tcagcagttt taccgatgcg ctattgccgt aataaggttc
     5401 attgaacgca aatggtgacg tcttaataaa cgtggagata aaccgacaat attgatgctc
     5461 gctgcgaagt ttttccgccg cccgggcagc gtaactacaa atggcctgcc gcatcgacgg
     5521 ataatccgtg atgcgttcac caaacgagcg ggaacagata atttcctgct tcgtcggtgc
     5581 aaactcttcc agttgcaaac agggttcacc gcgcagttca cgcaccgttc tttcgagcac
     5641 gacattaaaa tgtttacgga taaaccggat atctgaatct gccaaatcta gaacggtttt
     5701 gatccccatc gcgtccagtt ttttgctgat ccgccgtcca atcccccaga cttcatccac
     5761 ggggagagca gacattaatt tacgctggcg ttccagattt gataaatcca ccaccccacc
     5821 cgtctgccgc tgccattttt ttgccgcatg attggcaagc ttagccagcg ttttggtctg
     5881 ggcgatcccc acaccaacag taagatgggt acgttgtagc accgttgcgc gaatttctct
     5941 gccaaaatca gtcagatcgc gacaattatg cacacctgtc agatcgcaga atgcctcatc
     6001 aatactgtaa atctcgacgc ggggcgatag ttcttccagc gtcgacatca cccgattact
     6061 catgtctgcg taaagctcat aattgctgct aaagcaaacc acgccacagc gacgaaacag
     6121 atctttttgt ttgaaccagg gatcgcccat tttaacgccc agcgcctttg cctcagcgtt
     6181 tcgggcgata acgcaaccgt cattattcga tagcacaacc accggtttac cccataaatc
     6241 agggcgaaac accgtctcac agctggcata aaacgcgttt acatcacaga gggcaaacat
     6301 cagcgcatcg ccttaacgac gtggatcacc acaccaaaga catccagcgt atcttcgcta
     6361 ctgatggtaa tgggcgagta cgcactgttc ataggaataa gctgtaccgt cgggcgtagt
     6421 tgcaattttt tcaccgtaaa ctcgccgtca acagcagcga tgataatatc accatggctg
     6481 gcggtaatag cgctatcgac aatcagtaaa tcaccgtcac taatcccacc atcaatcata
     6541 gaatcaccac ttgctttgac gaaataagtc gcgctgggat gctggatcaa cagttgattc
     6601 agatcgatgc gctgttcaac gtaatctgcg gccggtgaag gaaagccaca ctgaacaaga
     6661 tcgctaaata gcggaaaagt cacaatttcg cggagatccg caggcttgat aaacaacata
     6721 ataatctgcc tgaagttata ctgtttttat atacagtagt ctgttcttgt cagcagatca
     6781 atactgattc aggctatcaa tatttgtcgc tgcatagact gctgattttt cgttctctta
     6841 tcctgtgctc acgcggcctt ctggcgacag cgtccatcca gcagaaagga aaaatatcac
     6901 ctggtttaaa atagaatcga agcattcgcc cagaaaggca ttctcattaa ggtactggat
     6961 gacggcatca agaaactgtg ttctattgct gcgggcgtag ttgaagggaa actgattcca
     7021 gaattgaaga acaagttaaa atctgtgcag agtttcttta ccaccctgtc taacacggtt
     7081 aaacaagcga ataaagatat cgatgccgcc aaattgaaat taaccaccga aatagccgcc
     7141 atcggggaga taaaaacgga aactgaaaca gccagattct acgttgatta tgatgattta
     7201 atgctttctt tactaaaaga agcggcccaa aaaatgatta acacctgtaa tgagtatcag
     7261 aaaagacacg gtaagaagac actctttgag atacctg
//
LOCUS       DAHHTG010000180         7280 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.183, whole genome shotgun sequence.
ACCESSION   DAHHTG010000180 DAHHTG010000000
VERSION     DAHHTG010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7280)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7280)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7280
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.183"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            complement(40..1497)
                     /gene="dtpC"
                     /locus_tag="NBI74_004155"
     CDS             complement(40..1497)
                     /gene="dtpC"
                     /locus_tag="NBI74_004155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpC"
                     /protein_id="HCD1934402.1"
                     /translation="MKTPSQPRAIYYIVAIQIWEYFSFYGMRALLILYLTHQLGFDDN
                     HAISLFSAYASLVYVTPILGGWLADRLLGNRTAVIAGALLMTLGHVVLGIDTNSTFSL
                     YLALAIIICGYGLFKSNISCLLGELYDENDHRRDGGFSLLYAAGNIGSIAAPIACGLA
                     AQWYGWHVGFALAGGGMFIGLLIFLSGHRHFQSTRSMDKKALTSVKFALPVWSWLVVM
                     LCLAPVFFTLLLENDWSGYLLAIVCLIAAQIIARMMIKFPEHRRALWQIVLLMFVGTL
                     FWVLAQQGGSTISLFIDRFVNRQAFNIEVPTALFQSVNAIAVMLAGVVLAWLASPESR
                     GNSALRVWLKFAFGLLLMACGFMLLAFDARHAAAGGQASMGVMISGLALMGFAELFID
                     PVAIAQITRLKMSGVLTGIYMLATGAVANWLAGVVAQQTTESQISGMAIAAYQRFFSQ
                     MGEWTLACVAIIVVLAFATRFLFSTPTNMIQESND"
     gene            complement(1556..3703)
                     /gene="cadA"
                     /locus_tag="NBI74_004156"
     CDS             complement(1556..3703)
                     /gene="cadA"
                     /locus_tag="NBI74_004156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase CadA"
                     /protein_id="HCD1934403.1"
                     /translation="MNVIAILNHMGVYFKEEPIRELHRALERLNFQIVYPNDRDDLLK
                     LIENNARLCGVIFDWDKYNLELCEEISKMNENLPLYAFANTYSTLDVSLNDLRLQISF
                     FEYALGAADDIANKIKQTTDEYINTILPPLTKALFKYVREGKYTFCTPGHMGGTAFQK
                     SPVGSLFYDFFGPNTMKSDISISVSELGSLLDHSGPHKEAEQYIARVFNADRSYMVTN
                     GTSTANKIVGMYSAPAGSTILIDRNCHKSLTHLMMMSDVTPIYFRPTRNAYGILGGIP
                     QSEFQHATIAKRVKETPNATWPVHAVITNSTYDGLLYNTDFIKKTLDVKSIHFDSAWV
                     PYTNFSPIYEGKCGMSGGRVEGKVIYETQSTHKLLAAFSQASMIHVKGDVNEETFNEA
                     YMMHTTTSPHYGIVASTETAAAMMKGNAGKRLINGSIERAIKFRKEIKRLRTESDGWF
                     FDVWQPDHIDTTECWPLRSDSTWHGFKNIDNEHMYLDPIKVTLLTPGMEKDGTMSDFG
                     IPASIVAKYLDEHGIVVEKTGPYNLLFLFSIGIDKTKALSLLRALTDFKRAFDLNLRV
                     KNMLPSLYREDPEFYENMRIQELAQNIHKLIVHHNLPDLMYRAFEVLPTMVMTPYAAF
                     QKELHGMTEEVYLDEMVGRINANMILPYPPGVPLVMPGEMITEESRPVLEFLQMLCEI
                     GAHYPGFETDIHGAYRQADGRYTVKVLKEESKK"
     gene            complement(3783..5117)
                     /gene="cadB"
                     /locus_tag="NBI74_004157"
     CDS             complement(3783..5117)
                     /gene="cadB"
                     /locus_tag="NBI74_004157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadaverine/lysine antiporter"
                     /protein_id="HCD1934404.1"
                     /translation="MSSAKKIGLFACTGVVAGNMMGSGIALLPANLASIGGIAIWGWI
                     ISIIGAMSLAYVYARLATKNPQQGGPIAYAGEISPAFGFQTGVLYYHANWIGNLAIGI
                     TAVSYLSTFFPVLNDPVPAGIACIAIVWVFTFVNMLGGTWVSRLTTIGLVLVLIPVVM
                     TAIVGWHWFDAATYAANWNTADTTDGHAIIKSILLCLWAFVGVESAAVSTGMVKNPKR
                     TVPLATMLGTGLAGIVYIAATQVLSGMYPSSVMAASGAPFAISASTILGNWAAPLVSA
                     FTAFACLTSLGSWMMLVGQAGVRAANDGNFPKVYGEVDSNGIPKKGLLLAAVKMTALM
                     ILITLMNSAGGKASDLFGELTGIAVLLTMLPYFYSCVDLIRFEGVNIRNFVSLICSVL
                     GCVFCFIALMGASSFELAGTFIVSLIILMFYARKMHERQSHSMDNHTASNAH"
     gene            complement(5483..7021)
                     /gene="cadC"
                     /locus_tag="NBI74_004158"
     CDS             complement(5483..7021)
                     /gene="cadC"
                     /locus_tag="NBI74_004158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylation/transport transcriptional
                     activator CadC"
                     /protein_id="HCD1934405.1"
                     /translation="MQQPVVRVGEWLVTPSINQISRNGRQLTLEPRLIDLLVFFAQHS
                     GEVLSRDELIDNVWKRSIVTNHVVTQSISELRKSLKDNDEDSPVYIATVPKRGYKLMV
                     PVIWYSEEEGEEIMLSSPPPIPEAVPATDSPSHSLNIQNTTTPPEQSPVKSKRFTTFW
                     VWFFFLLSLGICVALVAFSSLETRLPMSKSRILLNPRDIDINMVNKSCNSWSSPYQLS
                     YAIGVGDLVATSLNTFSTFMVHDKINYNIDEPSSSGKTLSIAFVNQRQYRAQQCFMSV
                     KLVDNADGSTMLDKRYVITNGNQLAIQNDLLQSLSKALNQPWPQRMQEMLQQILPHRG
                     ALLTNFYQAHDYLLHGDDKSLDRASELLGEIVQSSPEFTYARAEKALVDIVRHSQHPL
                     DEKQLAALNTEIDNIVTLPELNNLSIIYQIKAVSALVKGKTDESYQAINTGIDLEMSW
                     LNYVLLGKVYEMKGMNREAADAYLTAFNLRPGANTLYWIENGIFQTSVPYVVPYLDKF
                     LASE"
BASE COUNT         2092 a         1692 c         1669 g         1827 t
ORIGIN      
        1 gaaagttaat ttattcataa ccaattgttt ttacgacagt taatcgttgc tctcctgtat
       61 catattcgtc ggcgtgctaa acagaaaacg ggtagcaaag gccaatacca cgatgatcgc
      121 gacacaagcc aacgtccact ctcccatctg agaaaagaat cgctggtaag cggcaattgc
      181 cataccgcta atttgcgact ctgtcgtctg ctgtgccacg acgcctgcca gccagttggc
      241 gaccgcgcct gttgccagca tataaatacc ggttaatacg ccagacattt tcagacgcgt
      301 gatttgcgca atcgccaccg ggtcaataaa gagttcagca aagcccatta gcgccagtcc
      361 ggatatcatc acgcccattg acgcttgacc gccagccgct gcatgtcggg catcaaatgc
      421 cagcaacata aagccacaag ccatcagcag taggccaaag gcaaacttca gccagacgcg
      481 caatgctgaa ttgccgcggc tttctggcga cgccagccag gccagaacaa ccccagcgag
      541 catcaccgca atagcattca ccgactggaa tagtgctgta ggtacttcaa tattgaatgc
      601 ctgacgattc acaaagcgat cgataaacaa gctgatggta ctgccgccct gttgtgccag
      661 tacccagaac aacgtcccga caaacatcaa caatacaatt tgccaaagag cacggcggtg
      721 ttcggggaat ttgatcatca tgcgggcaat gatttgtgct gcaatgaggc aaacgatcgc
      781 cagcaaatat ccggaccagt cgttctccag cagcagggta aaaaatactg gggctaaaca
      841 gagcatcacc actaaccagc tccatactgg taaggcaaat ttgacgctag tgagcgcttt
      901 tttatccata ctacgcgtgg actggaaatg acgatgacca cttaagaaaa tcaacaaacc
      961 gataaacatg ccgcctcccg caagggcaaa gccaacatgc catccatacc actgagcagc
     1021 caggccgcag gcgatgggcg cggcgataga accgatattg cccgcagcat acagcagcga
     1081 aaaaccgcca tcacgtcgat gatcgttctc gtcgtagagc tcgccaagca aacagctgat
     1141 gtttgatttg aataaaccgt agccacaaat aatgattgcc agcgccagat acaggctaaa
     1201 ggttgaattt gtatcaatgc ccagcaccac atggccaagg gtcattaaca gcgcgccggc
     1261 aatcactgca gtgcggttgc cgagcaggcg gtcggcaagc cagccgccga gaataggggt
     1321 aacgtaaacc agagaagcat atgcgctgaa caggctgatg gcatggttat catcaaaacc
     1381 aagctgatgg gtgagataga gaatgagtaa ggcacgcatg ccgtaaaaac tgaagtactc
     1441 ccagatttgg atcgccacga tatagtatat cgcgcgcggc tgtgagggtg ttttcatgtg
     1501 ttctccttat gagcaaaaaa gggaagtggc aagccacttc ccttgtacga gctaattatt
     1561 ttttgctttc ttctttcaat accttaacgg tatagcggcc atcagcctga cggtatgcac
     1621 cgtgaatatc ggtttcaaag cccggatagt gagcgccgat ttcacacagc atctgcagga
     1681 actccagaac cggacggctt tcttcggtga tcatttcacc cggcattacc agaggaactc
     1741 ccggcggata cggaaggatc atattggcgt taatacgacc taccatttcg tcgaggtaaa
     1801 cttcttcggt cataccgtgc agctctttct ggaacgcagc atacggagtc attaccatag
     1861 tcggcagaac ttcaaatgcg cgatacatca gatccggcag attgtggtga acaatcagtt
     1921 tgtggatatt ctgagccagt tcctgaatac gcatgttttc atagaattca ggatcttcac
     1981 gatacagaga cggcagcatg tttttcacac gcaggttcag atcgaacgca cgtttgaagt
     2041 cagtcagagc acgcagcagg ctcagtgctt tggtcttatc gataccgatg ctgaacagga
     2101 acagcaggtt atacggaccg gttttctcaa caacgatgcc atgttcgtcg aggtatttcg
     2161 ccacgatgct ggccggaata ccaaagtcgc tcatggtgcc gtctttttcc atccccggag
     2221 tcagcagggt gactttgatc gggtcaagat acatgtgctc gttatcgatg tttttgaagc
     2281 cgtgccaggt gctgtcagaa cgcagcggcc agcattcagt cgtatcgata tgatccggct
     2341 gccatacatc aaagaaccag ccatcagatt ccgttctcag acgtttgatc tctttacgga
     2401 atttgatcgc acgttcaatg gaaccattga tcagacgttt acctgcattg cctttcatca
     2461 tcgctgcagc ggtttcagtg gacgccacga taccgtagtg cggagaggtg gtggtgtgca
     2521 tcatgtaggc ttcgttaaag gtttcttcgt ttacgtcacc tttaacgtgg atcatggaag
     2581 cctgagagaa cgccgccagc agtttgtgag tagactgggt ttcgtaaatc actttccctt
     2641 ctacacggcc accgctcata ccgcatttac cttcgtaaat cggtgagaag ttggtgtaag
     2701 gcacccacgc ggagtcaaag tggatggatt tcacatccag tgttttcttg atgaagtcgg
     2761 tgttgtacag cagaccatca taggtagagt tggtaattac agcatgtacc ggccaggttg
     2821 cgtttggtgt ttctttcacg cgcttagcaa tggtagcgtg ctggaattca ctctgtggga
     2881 taccaccaag aataccgtaa gcgttacggg tcgggcggaa atagattggc gtaacatcgc
     2941 tcatcatcat caggtgggtc agcgatttgt ggcagttacg gtcaatcaga atggtgctgc
     3001 ctgccggagc agagtacata ccaacaattt tgttcgcagt ggaagtaccg ttggtcacca
     3061 tgtagctgcg gtctgcgtta aagacgcgag cgatatactg ttctgcttct ttgtgtggac
     3121 cactgtgatc cagcagagaa cccaattcag atactgaaat ggaaatatca gatttcatgg
     3181 tgttcggacc aaagaaatca tagaacaggc tacctaccgg gcttttctgg aatgcagtac
     3241 cgcccatgtg accaggagta cagaaagtat atttaccttc acgaacatat ttaaacagtg
     3301 ctttggtcag cggaggaaga atagtgttga tatattcgtc agtggtctgc ttgatcttgt
     3361 tagcaatatc atcagcagca cccagcgcat attcaaagaa gctaatctgt aaacgcaggt
     3421 cattcaggct tacatcgaga gtggaatacg tattagcgaa cgcgtacaac ggcaggttct
     3481 cgttcatttt gctaatttct tcgcacagct cgagattata tttatcccag tcaaaaataa
     3541 cgccgcacag acgcgcattg ttttcgatca gttttaataa gtcgtcacgg tcgttcgggt
     3601 aaacaatctg gaagttcaga cgttcaagcg cgcgatgaag ttcacggatg ggttcttctt
     3661 taaaataaac ccccatgtga ttcaatattg caataacgtt catagtcata tctccaggta
     3721 aaaaaggccc ctcccaacac atgggacaaa atgaaaggag gagcctcgga aaatactttt
     3781 aattaatgtg cgttagacgc tgtgtggtta tccattgagt ggctctggcg ctcgtgcatt
     3841 ttgcgagcgt agaacatcag gataatcagg ctgacgatga aggtacctgc cagctcgaag
     3901 gagcttgcgc ccatcagcgc gatgaagcag aacacgcaac ccagaacaga gcagatcagg
     3961 ctgacaaagt tgcggatgtt aacgccttca aaacgaatca ggtcaacgca agagtagaag
     4021 tacggcagca tagtcagcag tactgcgata ccggtcagtt caccgaacag gtcagaggct
     4081 ttaccaccgg cagagttcat cagagtgatg aggatcatca gggcagtcat tttcactgca
     4141 gccagcagca gacctttttt cggaataccg ttgctgtcga cttcaccata aactttcggg
     4201 aagttaccgt cgttagcggc acgtacacct gcctggccta ccaacatcat ccaggagccc
     4261 agagaagtca gacacgcaaa ggcggtgaat gcagaaacca gtggcgcagc ccagttaccg
     4321 aggatagttg aagcactgat tgcaaacgga gcaccggaag ccgccattac agaagacgga
     4381 tacataccgg aaagcacctg agtcgcagcg atgtaaacaa tacctgctaa accagtaccc
     4441 agcatggttg ccagcggaac ggtacgtttc gggtttttaa ccataccagt acttactgct
     4501 gcggattcaa cacccacgaa ggcccacagg cagagcagaa tacttttaat gatcgcatga
     4561 ccatcagtgg tatccgcagt attccagtta gctgcataag ttgctgcatc aaaccaatgc
     4621 cagccaacaa tagcagtcat caccacagga ataagaacca gcaccagacc aatagtggtt
     4681 aaacggctta cccaggtacc gccgagcata tttacaaagg taaataccca gacgatagca
     4741 atacaggcga tacccgccgg aacaggatca tttaatactg ggaagaaggt ggaaagataa
     4801 gatacagcgg taataccaat tgccaggtta ccaatccagt tagcatggta ataaagaaca
     4861 cctgtctgaa aaccaaatgc aggggaaatt tctccggcat acgcaattgg gccaccttgt
     4921 tgcgggtttt ttgttgccag tcgggcatat acatatgcca gcgacattgc accaataata
     4981 gagataatcc aaccccagat agcaatacca ccgatacttg ctaggttcgc aggtaataat
     5041 gcaataccgc tccccatcat attaccggca acaacaccgg tacaggcaaa tagcccgatc
     5101 ttcttggcag aactcatgct cttctcctaa tttcattttt gaatttggag tccgggtcat
     5161 gatgtataac tatttcctga ccagaccaaa ctggcgctaa gattactcac gaaaaaagga
     5221 ttaatcctaa agattaggtg aaataacaca aaagtttctg taagtgagaa cttgaggttt
     5281 tttattaaca catcaggatc gcaagttgat atcatgaaaa gataaacatt caatgtttac
     5341 aatgcattgc gtgacattct ctggttaaat ttatgtaata aaaattatgc ggcaaataaa
     5401 ttgccgcaac atattatacc aacgggaaca tacaaaaact caacaacaaa tattcccgag
     5461 catgaatcaa cccgggagtt acttattctg aagcgagaaa tttgtcgaga taaggtacaa
     5521 cataaggaac agaagtctgg aatataccat tttcaatcca gtaaagggtg tttgcccctg
     5581 ggcgtaaatt aaaggcggtg agatatgcat cagctgcttc ccggttcatc cccttcattt
     5641 cataaacctt gccaagcaac acataattta gccaggacat ttcaagatca atgccggtat
     5701 ttatcgcctg ataagactca tctgttttac cttttaccag ggcactgacc gcttttattt
     5761 gatatataat ggacaggttg ttcaattccg gcagtgtaac aatgttatct atttctgtgt
     5821 tcagtgctgc taattgtttt tcgtctaaag gatgttgaga atggcgcacg atatcaacta
     5881 atgctttttc tgctctcgcg taggtaaatt ctggggatga ttgaacaatc tcacctaata
     5941 attcactggc acgatccaat gatttatcat caccatgcag taaataatca tgtgcctgat
     6001 aaaaattagt taataacgca ccacgatgcg gcaaaatttg ctggagcatc tcctgcattc
     6061 gttgtggcca cggttggttt aacgcttttg ataaactctg gagcaaatca ttttgaatcg
     6121 ccagctgatt accgttagtg atgacataac gtttatccag catggttgaa ccatctgcat
     6181 tgtctaccaa ttttaccgac ataaagcatt gttgagcacg gtattggcgc tgattaacaa
     6241 acgcaataga taatgtttta ccggaactgc tcggttcatc aatgttgtag ttgattttgt
     6301 catgcaccat aaaggtggag aaggtgttaa gtgatgtcgc caccaaatca cccacgccta
     6361 tcgcgtaaga gagctgatac ggagaactcc agctgttaca actcttatta accatattaa
     6421 tgtcaatatc gcgtggattg agcaaaatgc gcgatttact cataggaaga cgtgtttcaa
     6481 gacttgaaaa cgctaccagt gctacacaga tacctaacga caacaggaaa aaaaaccata
     6541 cccaaaaggt agtgaatcgt ttgcttttaa ctggggattg ttcaggtggc gttgtggtgt
     6601 tttgaatgtt aagactgtgg gagggagaat ctgtggcagg aaccgcctct ggtatggggg
     6661 gaggcgaaga tagcattatt tcctctccct cttcttcgct gtaccagata accggcacca
     6721 ttaatttata gccgcgcttt ggtacagtag cgatatagac aggactatct tcatcattat
     6781 cttttaatga cttacgtagt tctgagatac tctgcgtcac aacgtgattg gtgacaatac
     6841 ttctcttcca gacattatcg ataagttcat ccctgctaag tacttcgcca ctgtgttgag
     6901 caaagaaaac cagaagatcg attaatctcg gctcaagggt aagttgacgc ccattgcggc
     6961 taatttggtt tatggacgga gtaacaagcc attcgccaac gcgaactaca ggttgttgca
     7021 taatagaaac tcattcgaaa agggaatgat gcaatgataa ttgccataac ctattttttc
     7081 catctataga tgggtttatt tacatattat tggtgaatgc aagacgttat ttttacccgc
     7141 cataaacttc ttgattacat agtattacga aaggatttta ctgagaacca gaagtaattt
     7201 tccttaccat caaaattcat catctttgcc aaagaaaaat gttcagaaaa taatccatga
     7261 aaaattgtcc ggagcgctta
//
LOCUS       DAHHTG010000181         7203 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.184, whole genome shotgun sequence.
ACCESSION   DAHHTG010000181 DAHHTG010000000
VERSION     DAHHTG010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7203)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7203)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7203
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.184"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            complement(96..221)
                     /locus_tag="NBI74_004159"
                     /pseudo
     CDS             complement(96..221)
                     /locus_tag="NBI74_004159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000450676.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1627 domain-containing protein"
     gene            263..493
                     /locus_tag="NBI74_004160"
                     /pseudo
     CDS             263..493
                     /locus_tag="NBI74_004160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309956.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            545..1480
                     /gene="ttcA"
                     /locus_tag="NBI74_004161"
     CDS             545..1480
                     /gene="ttcA"
                     /locus_tag="NBI74_004161"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HCD1934406.1"
                     /translation="MQENQQITKKEQYNLNKLQKRLRRNVGEAIADFNMIEEGDRIMV
                     CLSGGKDSYTMLEILRNLQQSAPINFSLVAVNLDQKQPGFPEHVLPEYLETLGVEYKI
                     VEENTYGIVKEKIPEGKTTCSLCSRLRRGILYRTATELGATKIALGHHRDDILQTLFL
                     NMFYGGKMKGMPPKLMSDDGKHIVIRPLAYCREKDIQRFADAKAFPIIPCNLCGSQPN
                     LQRQVIADMLRDWDKRYPGRIETMFSAMQNVVPSHLCDTNLFDFKGITHGSEVVNGGD
                     LAFDREEIPLQPAGWQPEEDENQLDELRLNVVEVK"
     gene            complement(1608..2981)
                     /gene="dbpA"
                     /locus_tag="NBI74_004162"
     CDS             complement(1608..2981)
                     /gene="dbpA"
                     /locus_tag="NBI74_004162"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DbpA"
                     /protein_id="HCD1934407.1"
                     /translation="MTAFSTLNVLPPAQLTNLNGLGYLTMTPVQAAALPAILAGKDVR
                     VQAKTGSGKTAAFGLGLLQQIDASLFQTQALVLCPTRELADQVAGELRRLARFLPNTK
                     ILTLCGGQPFGMQRDSLQHAPHIIVATPGRLLDHLQKGTVSLDALNTLVMDEADRMLD
                     MGFSDAIDDVIRFAPASRQTLLFSATWPEAIAAISGRVQRDPLAIEIDSTDALPPIEQ
                     QFYETSSKGKIPLLQRLLSLHQPSSCVVFCNTKKECQSVCDALNEVGQSALSLHGDLE
                     QRDRDQTLVRFANGSARVLVATDVAARGLDIKSLELVVNFELAWDPEVHVHRIGRTAR
                     AGNSGLAISFCAPEEAQRANIISDMLQIKLNWQTPPANSSIVPLEAEMATLCIDGGKK
                     AKMRPGDVLGALTGDIGLDGADIGKIAVHPAHVYVAVRQAVAHKAWKQLQGGKIKGKT
                     CRVRLLK"
     gene            complement(3459..4442)
                     /gene="zntB"
                     /locus_tag="NBI74_004163"
     CDS             complement(3459..4442)
                     /gene="zntB"
                     /locus_tag="NBI74_004163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZntB"
                     /protein_id="HCD1934408.1"
                     /translation="MEAIKGSDVNVPDAVFAWMLDGRGGVKPLENTDVIDEAHPCWLH
                     LNYVHHDSAQWLATTPLLPNNVRDALAGESTRPRVSRLGEGTLITLRCINGSTDERPD
                     QLVAMRVYMDGRLIVSTRQRKVLALDDVVSDLEEGTGPTDCGGWLVDVCDALTDHSSE
                     FIEQLHDKIIDLEDNLLDQQIPPRGFLALLRKQLIVMRRYMAPQRDVYARLASERLPW
                     MSDDQRRRMQDIADRLGRGLDEIDACIARTGVMADEIAQVMQENLARRTYTMSLMAMV
                     FLPSTFLTGLFGVNLGGIPGGGWQFGFSIFCILLVVLIGGVALWLHRSKWL"
     gene            4697..5929
                     /gene="dgcM"
                     /locus_tag="NBI74_004164"
     CDS             4697..5929
                     /gene="dgcM"
                     /locus_tag="NBI74_004164"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcM"
                     /protein_id="HCD1934409.1"
                     /translation="MITHNFNTLDLLTSPVWIVSPFEEQLIYANSEARLLMQDLTFSQ
                     LRTGPYSVSSQKELPKYLSDLQNQHDIIEILTVQRNEEETALSCRLVLRELTETEPVI
                     IFEGIEAPATLGLKASRSTNYQRKKQGFYARFFLTNSAPMLLIDPSRDGQIVDANLAA
                     LNFYGYNHETMCQKHTWEINMLGRRVMPIMHEISHLPGGHKPLNFVHKLADGSTRHVQ
                     TYAGPIEIYGDKLMLCIVHDITEQKRLEEQLEHAAHHDAMTGLLNRRQFYHITEPGQM
                     QHLAIAQDYSLLLIDTDRFKHINDLYGHSKGDEVLCALARTLESCARKGDLVFRWGGE
                     EFVLLLPRTPLDTALSLAETIRVSVAKVSISGLPRFTVSIGVAHHEGNESIDELFKRV
                     DDALYRAKNDGRNRVLAA"
     gene            complement(5950..6513)
                     /gene="smrA"
                     /locus_tag="NBI74_004165"
     CDS             complement(5950..6513)
                     /gene="smrA"
                     /locus_tag="NBI74_004165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA endonuclease SmrA"
                     /protein_id="HCD1934410.1"
                     /translation="MNLDDKSLFLDAMEDVQPLKRATDVHWHPTRNQRAPQRIDTLQL
                     DNFLTTGFLDIIPLSRPLEFRREGLQHGVLDKLRSGKYPQQASLNLLRQPVEECRKMM
                     FSFIQQALADGLRNVLIIHGKGRDDKSHANIVRSYVARWLTEFDDVQAYCTALPHHGG
                     SGACYVALRKTAQAKQENWERHAKRSR"
     gene            complement(6708..>7203)
                     /locus_tag="NBI74_004166"
     CDS             complement(6708..>7203)
                     /locus_tag="NBI74_004166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000062809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCD1934411.1"
                     /translation="FSMYLFTPITHEWGWQELLFPQAIRGISQQFAMAPIVTLTLGGI
                     PKERLKLASGVFNLTRNLGGAIGIALCGSILNNRTNFHFSRMGEKMVSVPHTVNDFIS
                     RSALFFNRGGSDQTSEILASTKLLSQLMLREAQTMAFSDTFLLISGLLFIAFLLVPAM
                     NKSS"
BASE COUNT         1920 a         1835 c         1680 g         1768 t
ORIGIN      
        1 gaattatttt attcaccgaa aggtttcaca aaatatcccc tgatagaaaa ctaaaaaaca
       61 ctgcataata tcgctattct ggcacttgcg aaaaattata ttctgcacct gttgtagcgt
      121 tctatttctc tgcctggcaa cgaatgcaca atgtccgcca cagtttctgt tctaatttct
      181 tcctgtgtta ccgcatcctg tacgggtagc gcaacaccag catgctaagc aaggctttcc
      241 catcagattt cactacgact atttgggagc gagatggaat gcagcagtaa aacgtgctgg
      301 tattcgccgc cgtaatccgt accatacgcg acatactttt gcctgctggc tgttgacggc
      361 aggagcgaac ccggcattta tagccagcca gatggggcat gaaactgcgc agatggtgta
      421 tgaaatttac ggtatgtgga ttgatgacat gaacgacgaa caggtagcca tgttgaatgc
      481 gcggttatcg tagttgcaaa gtttgccctc aatatgcccc atttagtacc agagaactga
      541 aataatgcaa gaaaatcaac aaattacaaa gaaagaacaa tacaacctga acaaattaca
      601 aaaacgtctg cgtcgtaacg tgggcgaagc cattgctgac ttcaatatga ttgaagaagg
      661 cgatcgcatc atggtttgcc tctccggggg taaagacagc tataccatgc tggagattct
      721 gcgcaatttg cagcaaagcg cgccaatcaa tttttcgctg gtggctgtta acctcgatca
      781 aaagcaaccg ggcttcccgg aacacgttct gcccgagtat cttgaaacgc tgggcgttga
      841 gtacaagatt gttgaagaga acacttacgg tatcgtgaaa gagaagattc cggagggcaa
      901 aaccacttgc tcactgtgtt ctcgtcttcg tcgcggtatc ctttatcgca ccgcaacgga
      961 actgggggcg acgaagatcg cgctgggtca ccatcgtgac gatatcctgc aaacgctgtt
     1021 cttaaatatg ttctacggtg gcaagatgaa aggtatgcct ccgaaactga tgagcgatga
     1081 tggcaaacat atcgtgattc gtccgctggc ctactgccgc gagaaagata ttcagcgctt
     1141 tgccgatgca aaagcgttcc cgattattcc gtgcaacctg tgcggttcac agcctaacct
     1201 gcaacgtcag gtaattgctg acatgttgcg tgactgggat aaacgttatc cggggcgtat
     1261 cgagacgatg ttcagcgcaa tgcagaatgt ggtgccgtcg catctgtgcg ataccaacct
     1321 gttcgatttc aaaggcatta cccacggttc tgaagtggtt aacggggggg atctggcgtt
     1381 tgatcgcgaa gagatcccac tacaaccagc gggctggcag ccagaagaag atgaaaatca
     1441 gttggatgag ttacggctga atgtggttga agtgaaataa ccaggatagc gcccgatacg
     1501 caagcgtatc gggctactct tatggaagcc gataagacgc ggccagcgtc gcatccggca
     1561 atgccgaaga agatgtttac tcttgcaccc tgcaattcaa catttcatta ttttaataac
     1621 cgcacccggc acgtttttcc tttaatcttc ccgccctgta actgtttcca tgctttatga
     1681 gcaacagcct gacgcaccgc gacataaaca tgcgccggat gcacggcaat tttgccaata
     1741 tctgcgccat caagcccgat gtctcctgtc agcgcaccta atacatcacc cgggcgcatt
     1801 ttggcttttt tcccgccatc gatacacaac gttgccattt ctgcttccag cggcacaatg
     1861 gaactattcg ctggcggcgt ttgccagtta agttttatct gcaacatgtc agaaatgata
     1921 ttggcccgct gtgcttcttc cggagcacag aaactgatcg ccagaccgct atttcctgca
     1981 cgagctgtac gaccgatgcg atgtacatga acttcagggt cccacgccag ctcaaagttc
     2041 accaccagct caagcgattt tatatccaga ccacgcgcag caacatcagt cgcgaccagt
     2101 acacgggcgc taccgttagc aaaacgtacc agggtctgat cgcgatcgcg ttgctccaga
     2161 tcgccgtgta acgacaatgc actttgccct acttcattca gcgcgtcgca gacagactgg
     2221 cattcttttt tggtattgca aaacaccacg caagaggatg gctgatgcaa gcttaataac
     2281 cgttgcaaca gaggaatttt gcctttgctg gatgtctcat aaaattgttg ttcaataggt
     2341 ggcagagcat ctgttgagtc aatttcaatc gctaaaggat cgcgctgcac tcttccgctg
     2401 attgcggcaa tggcttccgg ccaggttgcc gaaaacagaa gtgtctgccg agatgctggc
     2461 gcaaaacgga tgacatcatc aatggcgtca ctaaatccca tatccagcat gcggtcggcc
     2521 tcatccatca ccagcgtatt caacgcatcc agtgatacag tgcctttttg caggtgatcc
     2581 agcaaacggc ccggcgttgc cacgataata tgcggcgcat gttgtaacga atcacgctgc
     2641 ataccgaacg gttgaccacc gcacaacgtc aaaattttgg tatttggcag aaaacgcgcc
     2701 agccgacgca attcacctgc cacctgatcc gccagttcac gcgtaggaca cagcactaaa
     2761 gcctgggttt gaaatagcga cgcatcaatt tgctgtaaca agccgaggcc aaaagccgcc
     2821 gttttgccgc tgccggtttt cgcctgcacg cgaacatctt ttccggcaag gatcgccgga
     2881 agcgcggcgg cctgcaccgg cgtcatggtt aaataaccca acccattaag gtttgtgagt
     2941 tgggcgggag gcaaaacatt cagggtagaa aaagcggtca caatctattc tcgtggtcat
     3001 cgacgcaaag tcagcaggcg cgtatcctcg cagatctacg ctcacgatgc gacaatttaa
     3061 tcggctcttc atcgggtggt gggtcaggca tgggttgcgg gcgagggatc ggatcgggca
     3121 cgggaacagg atcgccagga atcggttcag ggacaggaat ttgcaaataa ataagtgtcg
     3181 tcatatttcc ctccggtcat tgggtggact cttaaagggt agacgctgat aaataacagg
     3241 caaaaaaaag ccgactcatc aaagtcggcg tcgtacgaat caattgtgct atgcagtaat
     3301 tcaaaaaagg aagtaagaca atatggagcg caacgcccat cgcttgacgt tgcattcacc
     3361 tgcaagagag atattgccct gaatgggtag agagtttatt gacttcgctc aaactttacg
     3421 gcgtttttgt atacagacag ccgggaaaat tgcttttgtt acaaccattt actacgatgc
     3481 aaccataaag caacaccacc aataagaaca actaacagaa tacaaaaaat tgaaaatccg
     3541 aattgccatc caccgccagg gatcccacca aggttgacgc caaataaccc tgtcagaaac
     3601 gtactgggta aaaagaccat tgccatcaac gacattgtat aagtacgacg agctaaattt
     3661 tcctgcatca cctgagcgat ttcatccgcc atcacgccag tccgtgctat acaggcgtcg
     3721 atttcgtcaa gaccgcgccc aaggcgatcg gcaatatcct gcatccgacg gcgttggtca
     3781 tcgctcatcc acggcaaacg ttcactggca agacgagcat aaacatcacg ttgcggtgcc
     3841 atatagcgac gcatcacaat taattgtttg cgcagcagag ccaggaatcc acgcggtgga
     3901 atttgctgat cgagaagatt atcttcgagg tcgataattt tatcgtgcag ctgctcgata
     3961 aattcgctgg aatgatcggt caacgcatcg cacacatcaa ccagccaccc cccgcaatcg
     4021 gtcggaccag tgccctcttc cagatcgctc accacatcgt ccagcgccag cactttgcgt
     4081 tgtcgggtcg aaacaattaa ccgcccgtcc atatatacac gcatggcgac cagttgatcg
     4141 gggcgttcat cggtgctgcc gtttatacag cgcaatgtaa tcagcgtgcc ttcaccgaga
     4201 cggctgactc ggggccgtgt gctctcgccc gccagcgcat cacgtacgtt attgggaagc
     4261 agcggtgttg tcgccagcca ttgggcgcta tcatggtgta cataattaag gtggagccag
     4321 cagggatgcg cttcatcaat cacatctgta ttttccagcg gtttaacgcc gcctctacca
     4381 tccagcatcc atgcaaatac tgcatccggg acattaacgt ccgatccctt aatcgcttcc
     4441 acagtgcctc catcatcaac gcattatttt gtagtctagc cttctggccc tgttacgcaa
     4501 catctcatca ccccattacc ctgaaatgat taataaaatt ctgtctaaat tgaatacaaa
     4561 aagcaaaatg cttttccgta tacaaaccgt gtgaagtgtt aaatagcgtc tatcattatc
     4621 agaattatct gatcatatga cgtggctttt ttgcgatcgg atagcaacaa caattgataa
     4681 aaataatggg atctcaatga ttacgcacaa cttcaatacc ctggacttac tcaccagtcc
     4741 tgtctggatc gtttcgccct ttgaggaaca gttaatttat gccaacagcg aggcgcgact
     4801 gttgatgcaa gacctcacgt ttagtcagct acgaaccggt ccctattccg tctcctcaca
     4861 aaaagaactg ccgaaatacc tctccgatct gcaaaatcaa cacgatatta tcgaaatcct
     4921 caccgttcag cgcaatgaag aggaaacagc attaagctgt cggcttgttt tgcgagagct
     4981 gacagaaaca gaaccggtga ttattttcga aggtatcgaa gcaccggcaa cgctgggttt
     5041 aaaagccagt cgctcgacaa attatcagcg caaaaaacaa ggtttttatg cgcgcttttt
     5101 tctgactaac tctgcgccaa tgttgttgat tgacccctca cgagatggcc aaatcgttga
     5161 tgctaacctc gccgctctca atttctatgg ttataaccat gaaacgatgt gccagaaaca
     5221 tacctgggaa ataaatatgc tcgggcgtcg cgtcatgcct atcatgcatg aaatctcgca
     5281 tttacccggt ggtcataaac ctttgaattt tgttcataaa ctggcggatg gttcgactcg
     5341 tcatgtgcag acctatgccg ggccgattga aatttatggc gacaagctca tgttatgtat
     5401 tgtgcatgat attactgagc aaaaacggct ggaggagcag ctggaacatg ctgcccacca
     5461 tgatgcgatg acaggattac tgaatcggcg acagttttat cacattaccg agccaggcca
     5521 aatgcagcac ctcgccatcg ctcaggatta cagcttgttg ctcatcgaca ccgatcgttt
     5581 taaacacatt aacgatctct atgggcattc taaaggtgat gaggtgttat gcgcccttgc
     5641 ccgcactctc gaaagttgcg ctcgcaaagg cgatttggtg tttcgttggg gaggcgaaga
     5701 gtttgtctta ttgctaccaa gaaccccact ggataccgcg ctttcgctgg ctgaaactat
     5761 ccgcgtaagc gtggcaaaag tgagtatttc gggcttacca cgctttactg tcagcattgg
     5821 tgtggcgcat cacgaaggaa atgaaagcat cgatgaactg tttaaacgcg ttgatgatgc
     5881 tttgtatcga gcgaaaaatg atggacgcaa ccgcgtgctg gcggcataag ccacggatgc
     5941 gtctcgtgat caacgactgc gcttagcatg gcgctcccag ttttcttgct tcgcctgcgc
     6001 tgttttacgt agtgcgacgt aacacgcccc gctgccgcca tgatgcggta gcgcagtgca
     6061 atatgcctga acatcatcaa attcggtcag ccagcgcgcc acatagctgc ggacaatatt
     6121 ggcatgcgat ttatcatccc gtcctttgcc atgaataatc agcacgttac gcaaaccatc
     6181 cgccagggct tgttgaatga aactgaacat cattttgcgg cactcttcca ccggctggcg
     6241 caaaagattc aggctcgcct gttgcggata tttaccactg cgcagcttat ccagcacccc
     6301 atgttgcaac ccttcccgcc gaaactccag cggccgactt agcgggatga tgtcgagaaa
     6361 tccagtggtg aggaaattat caagttgcag cgtgtcgata cgctgcggcg cacgttggtt
     6421 acgcgttgga tgccagtgga catctgtagc acgtttcagc ggctggacat cttccatggc
     6481 gtcaagaaac agcgatttgt cgtcaaggtt catgtaacct cctatgcacc ttcacacgtc
     6541 gtgattcaga cgggttaatt gttcatactt actgcatcgt tcccttcaca gattgaaaca
     6601 atactctact tcatacctgt gagtatctgc gcaggcggag cattttatac tagcctgtta
     6661 gcagtatcga acagtttagc ccgagtttaa ttcagcacag gctccgatca ggatgattta
     6721 ttcatagcag gaaccaacaa aaatgcgata aataacagac cactgattag cagaaaggta
     6781 tccgaaaatg ccatagtttg cgcctccctc agcatcaatt gactcaataa tttggttgaa
     6841 gccagtattt ccgatgtctg gtcacttcct ccgcgattaa aaaacaaagc acttctggaa
     6901 atgaagtcat tcaccgtatg gggaacagaa accatttttt cacccattct cgaaaaatga
     6961 aaattagttc gattattgag aatagagcca cacagggcaa taccaatcgc gcccccaaga
     7021 ttacgtgtta aattaaatac accagaggct aacttcagcc gttcttttgg aattcccccc
     7081 agcgttaatg ttacgatggg ggccatcgca aactgctgcg aaatccctct aattgcctgt
     7141 ggaaacagta actcctgcca tccccattcg tgggttatag gggtaaaaag atacattgaa
     7201 aac
//
LOCUS       DAHHTG010000182         7124 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.185, whole genome shotgun sequence.
ACCESSION   DAHHTG010000182 DAHHTG010000000
VERSION     DAHHTG010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7124)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7124)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7124
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.185"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            complement(83..1747)
                     /gene="glnS"
                     /locus_tag="NBI74_004167"
     CDS             complement(83..1747)
                     /gene="glnS"
                     /locus_tag="NBI74_004167"
                     /EC_number="6.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase"
                     /protein_id="HCD1934412.1"
                     /translation="MSEAEARPTNFIRQIIDEDLASGKHTTVHTRFPPEPNGYLHIGH
                     AKSICLNFGIAQDYKGQCNLRFDDTNPVKEDIEYVDSIKNDVEWLGFHWSGNVRYSSD
                     YFDQLHAYAIELINKGLAYVDELTPEQIREYRGTLTQPGKNSPYRDRSVEENLALFEK
                     MRTGGFEEGKACLRAKIDMASPFIVMRDPVLYRIKFAEHHQTGNKWCIYPMYDFTHCI
                     SDALEGITHSLCTLEFQDNRRLYDWVLDNITIPVHPRQYEFSRLNLEYTVMSKRKLNL
                     LVTDKHVEGWDDPRMPTISGLRRRGYTAASIREFCKRIGVTKQDNTIEMASLESCIRE
                     DLNENAPRAMAVIDPVKLVIENYQGEGEMVTMPNHPNKPEMGSRQVPFSGEIWIDRAD
                     FREEANKQYKRLVLGKEVRLRNAYVIKAERVEKDAEGNITTIFCTYDADTLSKDPADG
                     RKVKGVIHWVSAAHALPVEIRLYDRLFSVPNPGAADDFLSVINPESLVIKQGFAEPSL
                     KDAVAGKAFQFEREGYFCLDSRHSTAEKPVFNRTVGLRDTWAKVGE"
     gene            complement(1967..2728)
                     /locus_tag="NBI74_004168"
     CDS             complement(1967..2728)
                     /locus_tag="NBI74_004168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCD1934413.1"
                     /translation="MKGYNRLEQIMDYLKSHNLVTVDELVAVTNASPATIRRDLIKLD
                     EQGVISRTHGGVTLNRFIPTQPTTHEKMQRSLAEKHAIASAAASMVKAGDSVVLDAGT
                     TMIELARQITHLPLRVITSDLHIALFLAEFKQIEVTIIGGRIDDSSQSCIGEHGRKLL
                     QNTWPDVAFLSCNSWDLEKGITAPTEEKAALKRDLIAHASRKILLADSSKYGSWSLFN
                     IAHLNELTEIITDSQLDGQTRRTLASLSTRLIIAD"
     gene            complement(2730..3716)
                     /locus_tag="NBI74_004169"
     CDS             complement(2730..3716)
                     /locus_tag="NBI74_004169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-threonate 4-phosphate dehydrogenase"
                     /protein_id="HCD1934414.1"
                     /translation="MNNIIAVTMGDPAGIGPEIIIKSLSEAGLAGAPVVVVGCVRTLR
                     RIMAMNITPQAELRVIQKVCDAHFAPGVINVMDEPLANPDALTPGVVQAVAGDLAYRC
                     IKRATALALSGEVKAIATAPLNKEALHLGGHNYPGHTELLAHLTGSKEYAMVLYTEKL
                     KVIHISTHISLRKFLDTLNGERVKTVIRVANHFLKRVGIERPRIAVAGVNPHAGEHGL
                     FGTEEIEIIAPAIEAMQAEDIDVTGPCPPDTVFMQCHEGLFDMVVAMYHDQGHIPLKL
                     LGFYDGVNITAGLPFIRTSADHGTAFDIAWTGKAKSESMAVSIQLAMQISRE"
     gene            complement(3709..4944)
                     /locus_tag="NBI74_004170"
     CDS             complement(3709..4944)
                     /locus_tag="NBI74_004170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four-carbon acid sugar kinase family protein"
                     /protein_id="HCD1934415.1"
                     /translation="MEKGITKPSILIVADDFTGANDAGVSLAQVGHTVDVAFEMPYRG
                     DASVWVINSDSRAMDPKLAAMKITSLMSHLPLANNPPLVIKKIDSTLRGNIGAEIEAL
                     MKACGITGAVVAPAFPQAGRTTVAGECWVNGVRITETEFASDPKTPVLSARIADIIRL
                     QTAIPCQPVTVSQLSHLSYEQPWIGVIDAQTDSDLDRIAAAVMQAKQPLLLVGSAGIC
                     DAVARRSAIMPPPTVLAIIGSMSEIAQRQIATLHSHPRITQIYVDVEHILAGNASDYD
                     ARIVQALQKGDHCIVHTCNDSVARHQIDTLCQRWQMSRAALGEKICRFLGELTRQVLL
                     CAMPDALYLSGGDVAMATASALGATGFRITGKVAQCVPYGHFLGGVWSRSVMTKAGGF
                     GDETTLHQVLNFIEEKRSE"
     gene            5114..6271
                     /locus_tag="NBI74_004171"
     CDS             5114..6271
                     /locus_tag="NBI74_004171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000234754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HCD1934416.1"
                     /translation="MVIINSTILSGAGAIPSLTSLLPDIRKMLLVTDRNIAQLDGVQQ
                     IRALLEKHCPQVNVIDNVPAEPTHHDVRQLMDALGDASFDVVVGIGGGSVLDVAKLLS
                     VLCHPQSPGLDALLAGEKPTQRVQSWLIPTTAGTGSEATPNAILAIPEQSTKVGIISQ
                     VLLPDYVALFPELTTSMPAHIAASTGIDALCHLLECFTATVANPVSDNAALTGLSKLF
                     RHIQPAVNDPQDLRAKLEMLWASYYGGVAITHAGTHLVHALSYPLGGKYHLPHGVANA
                     ILLAPCMAFVRPWAVEKFAQVWDCIPDAETALSAEEKSHALVTWLQALVNQLKLPNNL
                     ADLGVPPEDIASLSEAALNVKRLMNNVPCQLDLQDVQAIYQTLFPQHQFKE"
     gene            6275..>7124
                     /locus_tag="NBI74_004172"
     CDS             6275..>7124
                     /locus_tag="NBI74_004172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HCD1934417.1"
                     /translation="MPKTIEGVLTAIVTPFTATGSLNIPALKVQVNRQLEAGNAIFCG
                     GTNGEFFVLNEQEKLSVTQTCVDEVAGRAPVVAHIGEISTRETIRLGQQIEKLGVDAV
                     SVIAPYFVPLKQEELIAHYSAIADTLSIPMFLYNIPARTGNTIQPETARALASHPNII
                     GIKDSDGSDESLKGFLDAVRDIDGFNVLNGPDSLIHKGFVEGCSACISGLANVAPREI
                     NAIWSRFHAGDIEGSYEAQESVTGLRTDLYKVAFSPAAVKKALQLMGHDVGDSRYAVN
                     FTPEQIS"
BASE COUNT         1664 a         1923 c         1824 g         1713 t
ORIGIN      
        1 aacgccgcag agcgcggcgt ttgggataat ttgcatattt cttgccggat gcggcgtaaa
       61 cgctctatcc gacaacgtaa acttactcgc ctactttcgc ccaggtatca cgcagcccaa
      121 cggtgcggtt aaataccggt ttttccgccg tagaatggcg gctgtcgagg cagaagtaac
      181 cttcacgctc aaactggaat gctttacccg caaccgcatc tttcagcgac ggttcagcaa
      241 agccctgttt gatcaccagc gattccgggt taatcaccga caggaaatca tccgcagcac
      301 cagggttcgg cacgctgaac agacggtcat acaaacggat ttcaaccggc agcgcatgtg
      361 ccgcgctcac ccagtgaata acacctttga ctttacgacc atctgccgga tctttgctta
      421 aggtgtcggc gtcataagta cagaagatgg tggtgatatt gccttcggca tctttctcca
      481 cgcgttctgc cttaatgacg taagcattac gcagacgcac ttctttaccc agcaccagac
      541 gtttgtactg cttgttagct tcttcgcgga aatcggcgcg atcgatccaa atctcaccgc
      601 taaatggcac ctgacggctg cccatttccg gtttattcgg atggttcggc atggtaacca
      661 tttcgccttc gccctggtag ttttcgataa ccagtttcac cggatcgata accgccattg
      721 cgcgcggcgc attttcgttg agatcttcac ggatgcagga ttccagtgac gccatctcaa
      781 tggtgttgtc ctgcttggtc acaccaatgc gtttgcagaa ctcacgaata gaagccgcag
      841 tgtaaccacg acgacgcaga ccagaaatgg tcggcatacg cgggtcatcc cagccttcaa
      901 cgtgcttgtc ggtcaccagc aggttcagct tacgcttgga catcacagtg tattccagat
      961 tcaggcgcga gaattcgtac tggcgcgggt gaacaggaat ggtgatgttg tccagtaccc
     1021 agtcgtacag acgacggttg tcctggaact caagcgtaca cagagagtgc gtaatacctt
     1081 ccagcgcatc gctgatgcag tgggtgaagt cgtacatcgg gtagatgcac cacttgttgc
     1141 cagtctggtg gtgttcagca aacttaatgc ggtacagcac cggatcgcgc atcacgataa
     1201 acggcgaagc catgtcgatt ttcgcacgca ggcaggcttt accttcttca aaaccgccgg
     1261 tacgcatttt ctcgaacagc gccaggttct cttcaacgct gcgatcgcgg tacgggctgt
     1321 ttttacccgg ctgcgtcaag gtgccgcggt attcgcggat ctgttccggc gtcagttcat
     1381 caacgtacgc caggccttta ttgatcagtt cgatcgcata ggcgtggagc tgatcaaaat
     1441 aatcggagga gtaacggacg ttaccagacc agtgaaaacc taaccactct acgtcgtttt
     1501 tgatcgaatc gacatactcg atatcttctt ttaccgggtt agtgtcgtcg aaacgcaggt
     1561 tgcactggcc tttatagtcc tgggcgatcc cgaagttcag gcagatagat ttcgcatggc
     1621 caatatgcag atagccattc ggctccggcg ggaaacgggt gtgtactgtg gtgtgcttac
     1681 cactggccag atcttcatcg atgatctgac ggataaagtt agtcgggcgg gcttctgcct
     1741 cactcatcgt ggattcctca aagcgtaaac aacgtataac ggcgtatgat cttataagcg
     1801 ggacaggctg acaactgtta gttagcgaaa aaatggtttt gcgtaagata ttggggcgtt
     1861 ttctgcgcta ctgccagcag atcctcgcgc tggaaacgta aggccaaacg tcgccagcct
     1921 gacgcattta ttaaatttcc ggatgagctg gccgcagagt cgtagattag tcggctatga
     1981 ttaaacgcgt cgacaaggac gcaagcgttc tacgcgtctg cccatcaagc tgactatccg
     2041 taataatctc tgtcagttca ttaagatgcg cgatattaaa cagcgaccat gaaccgtatt
     2101 ttgagctgtc agccaggaga attttgcgcg aagcatgcgc gataagatcg cgttttagtg
     2161 cggctttctc ttccgtgggt gctgtaatac ccttttcaag atcccagctg ttacagctta
     2221 aaaatgccac gtccggccag gtattttgca gcaatttacg accatgttcg ccaatgcagg
     2281 actggctgga gtcgtcaata cgtccgccga taatcgtcac ttcaatttgc ttgaattcag
     2341 ccaaaaaaag cgcgatatgc aggtcgcttg tgatcacgcg aagcggtaaa tgtgtaatct
     2401 gccgcgcaag ttcaatcatt gtcgtaccgg catcaaggac gacggaatcc cccgctttga
     2461 ccattgacgc cgctgcgctg gcgatggcat gtttttccgc caggctgcgc tgcattttct
     2521 catgggtagt cggttgggta ggaataaacc ggttgagagt cacaccgccg tgtgtacggc
     2581 tgataacacc ctgctcatcg agttttatca ggtctcgacg aatcgtagcc ggcgacgcat
     2641 tggttaccgc caccagctca tctacggtga ccagattatg gcttttcagg taatccatga
     2701 tctgctctaa ccggttatat cccttcattt tattctcgcg aaatctgcat tgctaactgg
     2761 atggaaactg ccatgctctc agacttggct tttcctgtcc aggcaatatc aaacgcggtg
     2821 ccgtggtcgg cagaagtgcg aataaatggc aacccggcgg tgatattcac cccatcataa
     2881 aatccaagca gttttaaagg gatatgcccc tgatcgtggt acatagccac taccatatcg
     2941 aatagccctt catggcattg cataaacacg gtatctggcg ggcacgggcc agtaacatca
     3001 atatcttcgg cctgcattgc ttcaatcgca ggcgcgataa tctcgatttc ctcagtgccg
     3061 aataaaccat gttcccctgc atgtggattt acacccgcca cggcaatacg cggacgctca
     3121 ataccaacac gtttcaggaa gtggttcgca actcgaataa ccgttttgac cctttcacca
     3181 ttcaaagtat ccaggaattt acgcagtgag atgtgagtcg aaatatggat aaccttgagc
     3241 ttctcggtat agagcaccat cgcgtactct ttgctgccgg taagatgggc cagcaattcc
     3301 gtatgccccg gatagttatg cccgccaaga tgcagcgctt ctttattcag cggcgcagtg
     3361 gctatggctt tcacctcgcc tgataaggcc agagccgttg cacgtttgat acagcgatac
     3421 gctaaatcgc ctgccacagc ctgtaccact ccgggtgtca gcgcatcggg gttagccagc
     3481 ggctcatcca tcacgttaat cacgccggga gcaaaatggg cgtcgcacac cttttgaatg
     3541 acgcgtaact ctgcctgcgg cgtgatattc atcgccataa tacgtcgcag cgtgcgcaca
     3601 cagcccacca ccaccaccgg ggcaccagca agcccggctt cgctcaggga tttaataatg
     3661 atctccgggc caatacccgc cggatcgccc atcgttaccg caataatatt attcactacg
     3721 cttctcctcg ataaaattca gaacctgatg caacgtggtc tcatcgccaa acccaccggc
     3781 tttcgtcatc accgaacggg accagacgcc gcccagaaag tgaccgtatg gaacgcattg
     3841 cgccaccttc cccgtaatac gaaatccggt cgcgcctaac gcactggctg ttgccattgc
     3901 gacatcacca ccagagagat aaagcgcatc gggcattgcg caaagcagaa cttgccgggt
     3961 aagttcgcca agaaaccggc aaattttttc cccaagtgca gcgcgactca tctgccaacg
     4021 ctggcaaagg gtgtcgatct gatgccttgc tacagaatcg ttacaggtgt ggacaataca
     4081 gtggtcgcct ttctgcaagg cctgcacaat acgcgcgtca tagtcactgg cattgcctgc
     4141 cagaatgtgt tcaacatcga cgtatatctg tgtgatacgt gggtgtgaat gaagtgtggc
     4201 gatctgccgt tgagcgattt cgctcattga accaataatg gccagcacgg tgggtggcgg
     4261 cataatcgcg gagcgccggg caacggcatc acaaatacca gcggagccga ccagcagcag
     4321 cggttgttta gcctgcatca cagcggcagc gatgcgatcc aggtcgctgt ccgtctgcgc
     4381 atctatcacg ccaatccacg gttgctcata agacaaatgg ctgagctgag aaaccgtaac
     4441 gggctggcat ggaatggccg tctgcaaccg gatgatgtcc gcaattcggg cgctcaatac
     4501 cggggttttc ggatcgctag caaactcggt ttccgttatc cgcacaccgt ttacccaaca
     4561 ctcaccggcc actgtggtcc ttcccgcctg aggaaatgcg ggggcaacca ctgcgcccgt
     4621 tataccgcat gccttcatca gggcttcaat ttctgcgccg atattgcctc gcagcgtgga
     4681 atctatcttt ttaatcacga gcggcgggtt attcgccaga gggagatgac tcatcagcga
     4741 ggttattttc atcgccgcca gcttaggatc catcgcccga ctgtcgctgt taatcaccca
     4801 tacgctggca tcaccgcgat aaggcatttc aaaggccaca tcaactgtat gccccacctg
     4861 cgccaggctt acccccgcgt cgttggctcc cgtgaaatca tcggcaacga tgagaatgct
     4921 cggcttagtg attccctttt ccatcatcac tccaggttgt tttatgataa caatgattat
     4981 attcaatcat gattgattaa atgtgagcaa gctcaagtta ttaatcactc gaataaatta
     5041 taaaattaga ccatgttacg taggctacgg cattttgaat gattgaattc aatcaccaga
     5101 agagaggtta tttgtggtaa ttatcaatag cacgatactg agcggcgcag gcgctatccc
     5161 ttccctgacg tcgctcttac ccgacatcag aaaaatgctg ctggtcactg accgtaatat
     5221 tgcgcagctc gacggtgtgc agcagattcg cgccttactg gaaaagcact gcccgcaggt
     5281 taacgttatc gataatgtgc ccgcagagcc cacgcatcat gatgtgcgcc agctaatgga
     5341 tgcccttggc gatgcctctt ttgatgtggt ggtcgggatc ggcggtggca gcgtgttgga
     5401 tgtggcgaag ctgctatcgg tgctttgcca tccacaatca ccggggctgg atgcgctgct
     5461 tgcgggtgaa aaaccgactc agcgggtgca atcatggttg attcctacaa ccgccggaac
     5521 cggctcagaa gccacgccga atgcgattct ggcaatccct gagcaaagca cgaaggtggg
     5581 tattatttcc caggtgctgt taccagacta tgtggcgctt ttcccggaac tgaccaccag
     5641 tatgcccgcg catattgcgg cgtccacggg cattgatgct ctttgccact tactggagtg
     5701 ttttaccgcg accgtggcaa atccggtcag cgataacgcg gcgctgactg ggttaagtaa
     5761 acttttccgg cacattcaac ccgccgtgaa cgatcctcag gatctgcgcg caaaactgga
     5821 aatgctgtgg gcgtcttact atggcggcgt agcgataacc catgcgggca cgcatctcgt
     5881 tcatgcgctc tcctacccgt taggtggcaa atatcatctg ccgcatggcg tcgcgaatgc
     5941 catcttgctg gcgccgtgca tggcgtttgt tcgcccctgg gcggtcgaga aatttgccca
     6001 ggtctgggat tgcattcccg atgcggaaac cgccctgagc gcggaagaaa aatctcatgc
     6061 cctggtgacc tggttacagg cattagtcaa tcaactcaag ctacccaaca atctcgcgga
     6121 tctcggcgta ccgccagagg atattgcctc tctgagcgag gcggcactga acgtgaagcg
     6181 ccttatgaac aatgtgccgt gccagcttga tctacaggac gtacaggcca tttaccaaac
     6241 actgtttccg caacatcaat ttaaggaata aatcatgccg aaaaccattg aaggtgtatt
     6301 aaccgctatc gtcaccccgt ttaccgcgac cggcagtctg aatatcccgg cgttaaaagt
     6361 gcaggtgaat cgtcaactgg aagcgggtaa cgcgattttt tgcggcggca ccaacggcga
     6421 gttttttgtg ttgaatgaac aggagaagct cagcgttacg caaacctgcg tggatgaagt
     6481 ggctggtcgt gcgccagtcg tggcacacat tggcgagatc tctacgcgcg aaacgattcg
     6541 tctgggtcag cagattgaaa aacttggcgt agatgccgtg tcggtcattg ccccttactt
     6601 cgtaccgctg aaacaggaag agttgattgc gcattacagc gcaattgccg acacactgag
     6661 cattccgatg tttctctata acatcccggc ccgcactggc aatacgatcc agccagaaac
     6721 cgcgcgtgct ctggcgtcgc accccaatat cattgggatt aaagacagcg acggaagcga
     6781 cgaaagcctg aagggttttc tcgatgcggt gcgcgacatt gacggcttta atgtgctcaa
     6841 cggcccggat tcgctgatcc ataaagggtt tgtcgaggga tgttcagcct gtatctctgg
     6901 gctcgccaac gtcgcgccgc gagaaatcaa tgccatctgg tctcgcttcc atgccggtga
     6961 tattgaaggg tcttatgaag cacaggagag tgtaacgggc ctgcgtaccg atctttataa
     7021 agtagcattc tccccggctg cggtgaaaaa agcgttgcag cttatggggc atgatgttgg
     7081 tgatagccgc tacgcggtta atttcacgcc tgaacagatc agcg
//
LOCUS       DAHHTG010000183         7090 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.186, whole genome shotgun sequence.
ACCESSION   DAHHTG010000183 DAHHTG010000000
VERSION     DAHHTG010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7090)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7090)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7090
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.186"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            81..1316
                     /locus_tag="NBI74_004173"
     CDS             81..1316
                     /locus_tag="NBI74_004173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020237509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HCD1934418.1"
                     /translation="MVMIALFFIDTVAFIFMQLYFIYDRRQFSNCILSLAFLSCLIYF
                     VKTVIIIQQIIEGRLTSSVVQNDIAIYYLFRQMSLCMLIFLALINKVSENTKQRNLFS
                     KKVTLCISLFFVLGGPIVAHIFSSHYESYDLHIAELTNENGQVVWKASYVTIMIFMWL
                     TLLSVNLYFNGLRCDIWNGVSVIAFCAVLYNISLLFMSRYSVSIWYISRTIEVVSKLT
                     VLVIFMCHIFSALRVSKDIAHRDPLTNIFNRNYFFNELTVQSASAKKTPYCVMIMDID
                     HFKKVNDTWGHPVGDQVIKTVVNIIGKSIRPDDLLARVGGEEFGVLLTEIDTECAKAL
                     AERIRENVERLTGDNPEYAIPQKVTISIGAVVTQKNELNPKEIYQLADNALYEAKETG
                     RNKVVVKEAEILINRKDDE"
     gene            complement(1421..1508)
                     /locus_tag="NBI74_004174"
     tRNA            complement(1421..1508)
                     /locus_tag="NBI74_004174"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1472..1474),aa:Ser,seq:gga)
     gene            1742..2680
                     /gene="ghrA"
                     /locus_tag="NBI74_004175"
     CDS             1742..2680
                     /gene="ghrA"
                     /locus_tag="NBI74_004175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415551.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrA"
                     /protein_id="HCD1934419.1"
                     /translation="MDIIFYHPTFDTQWWIEALHKAIPQARVRAWKSGDNESADYALV
                     WHPPVEMLAGRDLKAVFALGAGVDSILSKLQAHPEMLKPSVPLFRLEDTGMGEQMQEY
                     AVSQVLHWFRRFDDYRLQQNSSHWQPLPEYHREDFTIGILGAGVLGSKVAQSLQTWRF
                     PLRCWSRTRKSWPGVQSFAGREELSAFLSQCRVLINLLPNTPETVGIINQQLLEKLPD
                     GAYLLNLARGVHVVEDDLLAALDSGKVKGAMLDVFNREPLPPESPLWQHPRVTITPHV
                     AAITRPAEAVDYISRTIAQLEKGERVCGQVDRARGY"
     gene            2735..3472
                     /gene="ycdX"
                     /locus_tag="NBI74_004176"
     CDS             2735..3472
                     /gene="ycdX"
                     /locus_tag="NBI74_004176"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding phosphatase"
                     /protein_id="HCD1934420.1"
                     /translation="MYPVDLHMHTVASTHAYSTLSDYIAQAKQKGIKLFAITDHGPDM
                     EDAPHHWHFINMRIWPRVVDGVGILRGIEANIKNVDGEIDCSGKMFDSLDLIIAGFHE
                     PVFAPHDKATNTQAMIATIASGNVHIISHPGNPKYEIDVKAVAEAAAKYQVALEINNS
                     SFLHSRKGSEDNCRAVAAAVRDAGGWVALGSDSHTAFTMGEFEECLKILDAVDFPPER
                     ILNVSPRRLLNFLESRGMAPIAEFADL"
     gene            3496..4050
                     /gene="ycdY"
                     /locus_tag="NBI74_004177"
     CDS             3496..4050
                     /gene="ycdY"
                     /locus_tag="NBI74_004177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone YcdY"
                     /protein_id="HCD1934421.1"
                     /translation="MNEFSILCRVLGSLYYRQPQDPLLVPLFTLIREGKLAANWPLEQ
                     DELLTRLQKSCDMTQVSADYNALFIGDECAVPPYRSAWVEGATEAEVRAFLSERGMPL
                     ADTPADHIGTLLLAASWLEDQSTEDESEALETLFSEYLLPWCGAFLGKVEAHATTPFW
                     RTMAPLTRDAISAMWDELEEDSEE"
     gene            4152..4643
                     /locus_tag="NBI74_004178"
     CDS             4152..4643
                     /locus_tag="NBI74_004178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HCD1934422.1"
                     /translation="MNILLSIAITTGILSGIWGWVAVSLGLLSWAGFLGCTAYFACPQ
                     GGLKGLAISAATLLSGVVWAMVIIYGSALAPHLEILGYVITGIVAFLMCIQAKQLLLS
                     FVPGTFIGACATFAGQGDWKLVLPSLALGLVFGYAMKNSGLWLAARSTKTAHREQQIK
                     NKA"
     gene            complement(4708..5541)
                     /gene="csgG"
                     /locus_tag="NBI74_004179"
     CDS             complement(4708..5541)
                     /gene="csgG"
                     /locus_tag="NBI74_004179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgG"
                     /protein_id="HCD1934423.1"
                     /translation="MQRLFLLVAVMLLSGCLTAPPKEAARPTLMPRAQSYKDLTHLPA
                     PTGKIFVSVYNIQDETGQFKPYPASNFSTAVPQSATAMLVTALKDSRWFIPLERQGLQ
                     NLLNERKIIRAAQENGTVAINNRIPLQSLTAANIMVEGSIIGYESNVKSGGVGARYFG
                     IGADTQYQLDQIAVNLRVVNVSTGEILSSVNTSKTILSYEVQAGVFRFIDYQRLLEGE
                     VGYTSNEPVMLCLMSAIETGVIFLINDGIDRGLWDLQNKTERQNDILVKYRHMSVPPE
                     S"
     gene            complement(5568..5984)
                     /gene="csgF"
                     /locus_tag="NBI74_004180"
     CDS             complement(5568..5984)
                     /gene="csgF"
                     /locus_tag="NBI74_004180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgF"
                     /protein_id="HCD1934424.1"
                     /translation="MRVKHAVVLLMLISPLSWAGSMTFQFRNPNFGGNPNNGAFLLNS
                     AQAQNSYKDPSYNDDFGIETPSALDNFTQAIQSQILGGLLSNINTGKPGRMVTNDYIV
                     DIANRDGQLQLNVTDRKTGQTSTIQVSGLQNNSTDF"
     gene            complement(6009..6398)
                     /gene="csgE"
                     /locus_tag="NBI74_004181"
     CDS             complement(6009..6398)
                     /gene="csgE"
                     /locus_tag="NBI74_004181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgE"
                     /protein_id="HCD1934425.1"
                     /translation="MKRYLRWIVAAEFLFAAGNLHAVEVEVPGLLTDHTVSSIGHDFY
                     RAFSDKWESDYTGNLTINERPSARWGSWITITVNQDVIFQTFLFPLKRDFEKTVVFAL
                     IQTEEALNRRQINQALLSTGDLAHDEF"
     gene            complement(6403..7053)
                     /gene="csgD"
                     /locus_tag="NBI74_004182"
     CDS             complement(6403..7053)
                     /gene="csgD"
                     /locus_tag="NBI74_004182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator CsgD"
                     /protein_id="HCD1934426.1"
                     /translation="MFNEVHSIHGHTLLLITKPSLQATALLQHLKQSLAITGKLHNIQ
                     RSLDDISSGSIILLDMMEADKKLIHYWQDTLSRKNNNIKILLLNTPEDYPYRDIENWP
                     HINGVFYAMEDQERVVNGLQGVLRGECYFTQKLASYLITHSGNYRYNSTESALLTHRE
                     KEILNKLRIGASNNEIARSLFISENTVKTHLYNLFKKIAVKNRTQAVSWANDNLRR"
BASE COUNT         1819 a         1556 c         1711 g         2004 t
ORIGIN      
        1 ttgggcttgc attggttttg gttaatagtt ggttcaatca gccaaccgtt gaggaagttg
       61 ttccacgatc aacctatctg atggttatga ttgctttatt ttttatcgat actgttgcat
      121 ttatttttat gcagttgtat ttcatttatg accgtaggca attttcaaac tgtatactta
      181 gtttggcttt tctaagttgt ttgatttact ttgttaaaac cgtcattatc attcagcaaa
      241 ttattgaggg gcgtttgaca agcagtgttg ttcaaaatga cattgcaatt tattatttgt
      301 ttcgtcagat gagtttgtgc atgttaatat ttctggcatt gataaataaa gttagtgaaa
      361 acacaaaaca gcgcaattta ttttccaaaa aagtgacttt gtgcataagt ttgttttttg
      421 ttcttggggg gccgatagtt gctcacatat tttctagcca ttatgagagc tacgatttac
      481 acattgccga gttaaccaat gaaaatggtc aggtggtttg gaaagcctca tatgttacca
      541 taatgatttt catgtggtta accttgcttt cggttaatct atactttaat ggtttacgct
      601 gtgatatctg gaacggggtg tcggtgattg ctttttgtgc cgtgctgtac aatatctctc
      661 tgttatttat gagcaggtat agcgtgtcca tttggtatat tagtcgcact attgaagtag
      721 tcagtaagtt aaccgttttg gtaattttta tgtgccatat attcagtgcg ctacgagtat
      781 caaaggacat tgcacatcgc gatcccttaa ccaatatatt caacagaaat tattttttta
      841 atgaactgac ggttcaatca gcatcggcca aaaaaacacc ttattgcgtc atgattatgg
      901 atattgacca cttcaaaaaa gtcaacgaca cctggggaca tccggttggc gatcaagtga
      961 taaagacagt ggtgaatatc attgggaaaa gcatacgacc agatgacctt ttagcgcgcg
     1021 ttggcggcga agagtttggc gtcttgctga cggagatcga tactgaatgt gcgaaagctt
     1081 tagcggaaag gattcgggaa aatgttgaac gtttaactgg cgataatcct gaatacgcca
     1141 taccacaaaa agtgacgatt agtattggcg ctgttgtaac gcagaaaaat gagttaaacc
     1201 ctaaggagat ctatcaactg gctgataacg cactttacga ggcgaaagag accgggcgta
     1261 ataaggtggt agtaaaagag gcagaaattc ttatcaaccg caaggatgat gaatgagtct
     1321 gtcgctagtt gtcaacctcc gcctggggct tctcatcccc ccggtgtatg caatatacga
     1381 aaaaaaagct cgtactttcg tacgagctct tctttaaata tggcggtgag ggggggattc
     1441 gaacccccga tacgttgccg tatacacact ttccaggcgt gctccttcag ccactcggac
     1501 acctcaccaa attgtcgttc ctgtcttgct ggaacgggcg ctaatttagg gaaatcatga
     1561 cctgaggtca acaaactttt ttaaaaaatc gctcgattat tcaaacttca atcaatatgt
     1621 agttttaata agcgaaattt gctttttttg tcaccgacca cggatttgtt atgctggtgg
     1681 cctttgtaga tcataacgat aagtgcgaat aaatttcgcg caacgctttt cgggagtcag
     1741 tatggatatc atcttttatc acccaacgtt cgatacccaa tggtggattg aggcactgca
     1801 caaagctatt cctcaggcaa gagtcagagc atggaaaagc ggagacaatg aatcagccga
     1861 ttatgcttta gtctggcatc ctcctgttga aatgctggca gggcgcgatc ttaaagcggt
     1921 gtttgcactt ggggcaggtg ttgattctat tttgagcaag ctacaggctc accctgaaat
     1981 gctgaagcct tctgttccac tttttcgcct ggaagatacc ggtatgggcg agcaaatgca
     2041 ggaatatgct gtcagtcagg tgctgcattg gtttcgacgt tttgacgatt accgcctcca
     2101 gcaaaatagt tcgcattggc aaccgctgcc tgaatatcat cgggaagatt ttaccatcgg
     2161 cattttgggc gcaggcgtac tgggcagtaa agttgcacag agtctgcaaa cctggcgctt
     2221 cccgctgcgt tgctggagtc gaacccgtaa atcgtggcct ggcgtgcaaa gctttgccgg
     2281 acgggaagaa ctttctgcat ttctgagcca atgccgggta ttgattaatt tgttaccgaa
     2341 tacccctgaa accgtcggca ttattaatca acaattactc gaaaaattac cggatggcgc
     2401 gtatctcctc aacctggcgc gtggtgttca tgtcgtggaa gatgacctgc tcgcggcgct
     2461 ggatagcggc aaagttaaag gcgcaatgct ggatgttttt aatcgtgaac ccttaccgcc
     2521 ggaaagtccg ctctggcaac atccacgcgt gacgataaca ccacatgtcg ccgcgattac
     2581 ccgtcccgct gaagctgtgg attacatttc gcgcaccatt gcccagctcg aaaaagggga
     2641 gagggtctgc gggcaagtcg accgcgcacg cggctactaa taaagcatca ggtttcctgc
     2701 tatccttggc gggaattgaa tacaggagag agttatgtat cccgtcgacc ttcatatgca
     2761 taccgttgcc agcacgcatg catatagcac attgagtgat tacattgccc aggccaaaca
     2821 aaagggcatt aaactttttg cgatcaccga tcatggcccg gatatggaag atgcgccgca
     2881 tcactggcac ttcattaaca tgcgtatctg gccgcgagtg gttgatgggg tagggatcct
     2941 gcgcggcatc gaagctaaca ttaaaaatgt tgatggtgaa attgattgca gcggcaaaat
     3001 gtttgattcg ctggatctga ttattgccgg ttttcatgag ccggtttttg cgccacatga
     3061 caaagcaacc aatacacaag cgatgatcgc cactatcgcc agcggcaatg tgcatataat
     3121 aagccatccc ggaaatccga aatatgagat agatgttaaa gccgttgctg aagcagccgc
     3181 gaaatatcag gtggcgctgg aaatcaataa ttcctcattt ttacactcac gtaagggcag
     3241 tgaagacaac tgtcgtgcgg tagctgcagc ggtacgtgac gctggaggtt gggtggcatt
     3301 aggctcggat tctcacactg catttaccat gggggaattt gaagagtgtc ttaaaatcct
     3361 cgacgcggta gattttccgc cagaacgcat tttgaatgtt tctccgcgcc gcttactgaa
     3421 cttccttgaa tctcgcggta tggcaccgat tgcggaattt gcagaccttt aattactagt
     3481 aatggaaata tattaatgaa cgagttttct atcctctgtc gtgtgctggg ttcgctctat
     3541 taccgccaac cgcaagatcc tttactggtg ccgttgttta ccctgattcg tgaggggaaa
     3601 ctggctgcga actggccact ggagcaggat gagttactga cgcgtttaca gaaaagctgt
     3661 gatatgacgc aagtctctgc cgattacaat gcgttgttta tcggcgatga atgtgctgtg
     3721 ccgccatatc gtagcgcatg ggttgagggc gcaacggaag cggaagtgcg cgcttttctt
     3781 tccgaacgag ggatgccact agcggatacg ccagccgatc acatcggcac attgctgctt
     3841 gcggcttcct ggctggaaga tcagtcaacg gaagatgaga gcgaagcact ggaaacactg
     3901 ttcagtgagt atctgttacc ctggtgtggt gcgttccttg gcaaagtgga ggcccatgca
     3961 actacgcctt tctggcgcac catggcaccg ctaacccgcg atgctattag tgcaatgtgg
     4021 gacgagctgg aagaagattc tgaagagtaa atgtgatctg catcactcat aactgcaact
     4081 gataatttgt tgttgcataa aatgtgtgct cgatctcatt catggccgcg ttttctgcta
     4141 tcatgcgcgg catgaacata cttctctcta ttgcaatcac aacaggcatt ctctccggta
     4201 tctggggatg ggtggctgtt tccctgggct tactaagctg ggcgggcttc ctgggctgta
     4261 cggcctactt tgcctgcccg caaggtgggc tgaaagggct ggcgatctcc gctgcaaccc
     4321 tgcttagtgg cgtggtgtgg gcgatggtca ttatttacgg tagtgcactg gcaccacatc
     4381 tggaaattct cggttatgta ataaccggta ttgtcgcgtt tctgatgtgt attcaggcca
     4441 aacagctgct gctttcattt gttccgggga cgtttatagg cgcatgcgcg acgtttgccg
     4501 ggcaggggga ctggaaactg gtgttacctt cgctggcgct tggactggta tttggttacg
     4561 caatgaaaaa cagcggcctg tggctggcgg cgcgtagtac gaagaccgct caccgtgagc
     4621 agcaaatcaa aaataaagcg tgaggggcac tcacgctttc gcttaaacag taaaatgccg
     4681 gattattaat tccggcgttt ttatctatca ggattccggt ggaaccgaca tatggcggta
     4741 tttcaccaga atgtcattct gccgttctgt tttattttgc aaatcccaaa ggccgcggtc
     4801 gataccatca ttaatcagga aaatgacgcc tgtttcgata gccgacatca ggcacagcat
     4861 aacgggttcg ttcgaggtgt aacctacttc cccttccagc aagcgctggt agtcaataaa
     4921 gcggaagacc cccgcctgaa cttcatagga aagtatcgtc ttactggtgt tcaccgagga
     4981 gaggatctca ccggtactca cattgacgac acgcaggttc acggcaatct gatcaagctg
     5041 gtattgcgtg tctgcaccga tcccaaaata tcttgcccca acaccgcccg atttaacgtt
     5101 gctttcataa ccgataatcg aaccttcaac catgatattt gccgccgtta aagattgcag
     5161 cgggattcgg ttattaatgg caactgtgcc gttttcttgt gccgcacgaa taatcttgcg
     5221 ctcgttaagc agattttgta agccctggcg ctccagcggt ataaaccagc gagaatcttt
     5281 cagtgccgtg accagcattg ccgtggcgct ttgcggaaca gcagtggaga agttacttgc
     5341 cgggtagggt ttaaattgtc cggtttcgtc ctgaatgttg tataccgaaa caaagatctt
     5401 acccgtcggc gctggcagat gggtcaaatc tttgtagctc tgagcacgag gcattaatgt
     5461 cggtctggcg gcttctttag gcggggcggt taagcatccg ctcagtaaca tgacggcaac
     5521 caaaagaaat aagcgctgca tgattatttt ccttatgaag ctggggctta aaaatcggtt
     5581 gagttatttt gtaaacccga aacctggatg gtcgaggttt gtccggtttt acgatctgtc
     5641 acgttcaact gcaattgacc atcgcggtta gcaatatcaa caatataatc gttggtcacc
     5701 atgcggcccg gtttaccggt attaatattc gacagtagcc cacccaaaat ttgtgactgg
     5761 atggcctgag taaagttatc taacgctgat ggcgtttcaa taccaaagtc atcgttatag
     5821 ctcggatctt tataagagtt ttgtgcctga gcgctattta acaaaaaagc gccattattt
     5881 gggttaccac caaagtttgg attacggaac tggaaagtca tggatccagc ccaacttaat
     5941 ggcgaaataa gcatgagtag aactactgca tgtttgacac gcattgcagc ctccgaacaa
     6001 tttattattt aaaattcatc atgcgccaaa tcgcccgtac ttaataacgc ctggtttatc
     6061 tggcgacgat ttagtgcttc ttcagtttga atcagtgcaa agacgacagt tttctcgaag
     6121 tctcttttca acggaaataa aaaagtctgg aaaataacgt cctgattgac cgttatagtg
     6181 atccaacttc cccatcgtgc actgggcctt tcattaatcg ttaagttacc cgtatagtca
     6241 ctttcccatt tatcactaaa ggctcggtaa aaatcatggc caatagatga aacagtatgg
     6301 tcagttaaca atcccgggac ttctacctca acggcatgaa gattccctgc ggcgaacaga
     6361 aattctgccg ccacaatcca gcgtaaataa cgtttcatgg ctttatcgcc tgaggttatc
     6421 gtttgcccag gaaaccgctt gtgtccggtt ttttacggct atcttcttga aaagattata
     6481 aagatgcgtt ttaaccgtat tttcgctgat gaacaacgaa cgagcgatct cgttattaga
     6541 cgcgccgata cgcagcttat tcaggatctc tttttcccga tgagtaagga gggctgattc
     6601 cgtgctgtta taacgatagt tacctgaatg cgtaatcagg taactggcaa gcttttgcgt
     6661 aaagtagcat tcgccgcgca ggacgccttg caacccattg acaacacgtt cttgatcctc
     6721 catggcataa aaaacgccgt tgatatgagg ccagttttca atgtcgcggt acgggtaatc
     6781 ttcaggcgta tttagcaaca atattttgat attgttgttt ttcctgctca aagtatcctg
     6841 ccaataatgg ataagctttt tatccgcttc catcatatcc agaagaataa tagagcctga
     6901 agatatatcg tccagagaac gttgaatatt atgtaatttt cctgtaatcg ccagcgattg
     6961 tttaaggtgc tgcaagagag ctgtcgcttg caaagaaggt ttagtgatca acaataatgt
     7021 atgaccatga atactatgga cttcattaaa catgatgaaa ccccgctttt ttattgttcg
     7081 cacacctgac
//
LOCUS       DAHHTG010000184         6956 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.187, whole genome shotgun sequence.
ACCESSION   DAHHTG010000184 DAHHTG010000000
VERSION     DAHHTG010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6956)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6956)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6956
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.187"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            82..285
                     /locus_tag="NBI74_004183"
     CDS             82..285
                     /locus_tag="NBI74_004183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001309419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
                     /protein_id="HCD1934427.1"
                     /translation="MVERTMAFKHYDVVRAASPSDLAEKLTHKLKEGWQPFGSPVAIT
                     PYTLMQAIAAEGDVVVSGATEPE"
     gene            282..443
                     /locus_tag="NBI74_004184"
     CDS             282..443
                     /locus_tag="NBI74_004184"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1934428.1"
                     /translation="MILRHRTGPAFQAIKDGLAVGLNALTLTDITKNATYGVEIESLV
                     LEINAPASS"
     gene            593..808
                     /locus_tag="NBI74_004185"
     CDS             593..808
                     /locus_tag="NBI74_004185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HCD1934429.1"
                     /translation="MYQMEKITTGVSYTTSAVGTGYWFLQLLDRVSPSQWAAIGVLGS
                     LLFGLLTYLTNLYFKIKEDRRKAARGE"
     gene            813..1703
                     /locus_tag="NBI74_004186"
     CDS             813..1703
                     /locus_tag="NBI74_004186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001037014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdfR family protein"
                     /protein_id="HCD1934430.1"
                     /translation="MNKKYELVVKGINNYGDKVTVTVKSEGDGQASLLLPDVAISLDR
                     TEGATLEFYEAEAKKQAKQFFMDVAAGLCEWNEPLPEKRPVILEAQDVLITYKGKLPG
                     RITCSLKMPPSTLRSEKDDVESRIEKLESYVVELNKKWSILVPSGDEKQFAAFDDYCR
                     KVMSRNLAECFSIHNDNFSDPEWECNRPSFVVSGDAGKITISENGKVTPPSHQHSEEL
                     IEFAIDYLKNNKKQGLMKRVGRCMGYLQVAAEIEALASGADKDAIVREALLRDFNTPP
                     FKKGPAYWLHPGLTYLKVRI"
     gene            1740..2273
                     /locus_tag="NBI74_004187"
     CDS             1740..2273
                     /locus_tag="NBI74_004187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HCD1934431.1"
                     /translation="MNTKIKYGLSAAVLALIAAGAPAPDILDQFLDEKEGNHTTAYRD
                     GAGIWTICRGAIMVDGKPVVPGMKLSKEKCDQVNAIERDKALAWVEKNIRVPLTEPQK
                     AGIASFCPYNIGPGKCFPSTFYRRINAGDRKGACEAIRWWIKDGGRDCRIRSNNCYGQ
                     VSRRDQESALACWGIDR"
     gene            2430..2612
                     /locus_tag="NBI74_004188"
     CDS             2430..2612
                     /locus_tag="NBI74_004188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310767.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1934432.1"
                     /translation="MAKCKRILLRLTMKNGLELKAPVTDDISRALAFAIKWVAVGVAV
                     SPMLYGLAKLVIALKS"
     gene            2627..2758
                     /locus_tag="NBI74_004189"
     CDS             2627..2758
                     /locus_tag="NBI74_004189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1934433.1"
                     /translation="MSDKLITLTKILCVIVGISFSLMLVALFLSMAWMMLSSSGLLG"
     gene            2761..3228
                     /locus_tag="NBI74_004190"
     CDS             2761..3228
                     /locus_tag="NBI74_004190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HCD1934434.1"
                     /translation="MNRMLSAFIVILLVACGALWMATDHYRDNAITYKAQRDNKASEL
                     KLANATITDMQVRQRDVAALDAKYSRELADARAENETLRADVAAGRKRLRINATCPGS
                     VREAPTTSGVDNATGPQLADTVTRDYFTLRERLMTMHKQLEGAQDYIRTQCLK"
     gene            3539..3865
                     /locus_tag="NBI74_004191"
     CDS             3539..3865
                     /locus_tag="NBI74_004191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000830178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HCD1934435.1"
                     /translation="MKRRLLLLFLLSVLAVGCSQQKADEPRQLVTVYPRYPEYAAANY
                     IKGLVEVKFDIGADGTVTRIVFLRSEPHNLFRDEVVKAMAKWRFEKNRPCQGVKRQFI
                     FTPSRP"
     gene            4072..4209
                     /locus_tag="NBI74_004192"
     CDS             4072..4209
                     /locus_tag="NBI74_004192"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1934436.1"
                     /translation="MLAVFQLVKNTCSRLLQCLQHAAAMRLAFTNHLSFLYQSLRELI
                     M"
     gene            4824..5333
                     /locus_tag="NBI74_004193"
     CDS             4824..5333
                     /locus_tag="NBI74_004193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HCD1934437.1"
                     /translation="MATQTEVARHLSLTDRQLRRLQKLPGAPISNKRGQLDLDAWRDF
                     YISYLRRSKNDVPDGDSEDDYEEKLLIARWELTAEQAVTQQLKNEVSKGKLIDTGFCI
                     FALSKLAMALSSTLDSIPLSMQRQFPDLTPRHLDHLKTLIAKGANQCARAGDKLPDLL
                     DEYIRATTE"
     gene            5305..>6956
                     /locus_tag="NBI74_004194"
     CDS             5305..>6956
                     /locus_tag="NBI74_004194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HCD1934438.1"
                     /translation="MNISEQQLNNMMSAVTTALQPLIRALPVTPVEWADQNYYLPKES
                     SYGEGEWKTLPFQIAIMNSMGNDQIRTVNLIKSARVGYTKMLLGVVGYFIEHKSRNSL
                     LFQPTDSAAEDFMKSHVEATIRDVPCLKDLFPWLGRKHRDNTLTLKRFSSGVGFWCLG
                     GAAAKNYREKSVDVVCYDELSSFEPDVEKEGSPTLLGDKRIEGSVWPKSIRGSTPKIK
                     GTCQIEKAANESAHFMRFYVPCPHCGEEQYLKFGDESTPFGLKWEKDSPESVFYLCEH
                     HGCVIHQSELDQSNGRWICENTGMWTRDGLTFFSAADNEIPPPRSITFHIWTAYSPFT
                     TWVQIVYDWLDALKDPNGLKTFVNTTLGETWEEAVGEKLDHQVLMDKVVRYTAAVPAR
                     VVYLTAGIDSQRNRFEMYVWGWAPGEEAFLVDKIIIMGRPDEEETLLRVDAAINKKYR
                     HADGTEMTISRVCWDTGGIDGEIVYQRSKKHGVFRVLPVKGASVYGKPVITMPKTRNQ
                     RGVYLCEVGTDTAKEILYARMKAEPTPADEATSYAIRFPDDP"
BASE COUNT         1785 a         1487 c         1954 g         1730 t
ORIGIN      
        1 gatgatgagg tttttattta aggtatggtt ttgtgttttt tctgtattac atgtcaggta
       61 ttttaaagaa tcatttttca gatggtggaa agaaccatgg catttaaaca ctatgatgtt
      121 gtcagggcgg cgtcgccgtc agatcttgcg gaaaagctga cacataaact gaaagagggc
      181 tggcagccgt ttggtagtcc ggtggccata accccttata ccctgatgca ggcgattgca
      241 gcagaaggtg atgtggtggt cagtggtgca actgagccgg agtgatacta cgtcatcgta
      301 ctggcccggc attccaggcc ataaaagacg gtctggcagt gggactaaat gcactgacgc
      361 tgacggatat taccaaaaat gcaacgtatg gcgttgagat agaaagtctg gtgctggaga
      421 taaatgcacc ggcatcatca taaaaagtga gccagtcaaa tggaaggtat cgttaaactc
      481 accggtagtg tcagtgggtc gtctgagacg cctgcatgag ttatcagagc catcagtact
      541 taactggtgg cttttttatt gttgtcagct tccggataac gggagacggg gtatgtacca
      601 gatggaaaaa atcacaacag gtgtgtcata caccacgtca gcggtgggaa cgggctactg
      661 gttcctgcag ttgctggaca gggtttcccc gtctcagtgg gcggcaatag gcgtgctggg
      721 gagtctgctg tttgggctgc tgacatatct gactaacctg tatttcaaaa tcaaagagga
      781 ccggcgtaag gcggcgcggg gagagtaaag tgatgaataa aaaatatgaa ctggttgtta
      841 aggggataaa taattacggg gataaggtta ctgttactgt gaagtcggaa ggtgacgggc
      901 aagcgtcgct gttgttgcca gatgtggcga ttagtcttga ccgtactgaa ggtgccacgc
      961 tggagtttta cgaagctgag gcgaaaaagc aggcgaagca gtttttcatg gatgttgctg
     1021 ccgggttatg tgaatggaac gaaccgttgc cggaaaagcg ccccgtaatt ttagaggcgc
     1081 aggatgtgtt gataacctac aaaggaaagc taccgggaag aattacttgt tctctgaaga
     1141 tgccgccgtc aacactgcgg tcagaaaaag atgatgttga atcacgtatt gaaaaactgg
     1201 agagctacgt cgttgagctg aataagaaat ggtcgatatt ggtgccttct ggcgatgaaa
     1261 agcagtttgc tgcgtttgac gattattgtc ggaaagtgat gagcagaaat ctcgcagagt
     1321 gtttcagtat tcataatgat aatttcagtg acccggaatg ggagtgtaac cggccatcct
     1381 ttgttgtatc cggtgatgct gggaaaataa ccatctcaga aaatgggaaa gtaacacctc
     1441 catcgcacca gcacagtgag gagctcattg aatttgccat tgattacctg aagaacaata
     1501 aaaagcaggg gctgatgaag cgcgttggcc gttgcatggg atatcttcag gtagccgctg
     1561 agattgaagc gctggccagt ggtgctgata aggatgcaat tgtgcgggag gctcttcttc
     1621 gtgattttaa tactccaccc tttaaaaaag ggccggctta ctggcttcat ccggggctga
     1681 cttatcttaa agtgcgtatt tagtgggcca gggacagcgg ctgaatattt aatatatcca
     1741 tgaacaccaa aatcaaatac ggcctgtcgg ctgccgttct ggcgctgatt gccgctggtg
     1801 cgcctgcgcc tgacattctc gaccagtttc tggatgaaaa ggaaggcaac cacaccacgg
     1861 cataccgtga tggtgcaggt atctggacca tctgccgtgg tgccatcatg gtggatggca
     1921 aacctgtcgt tccgggcatg aagttgtcga aggaaaaatg cgaccaggtt aacgccattg
     1981 agcgtgataa agcgctggcg tgggtggaga aaaacatcag agtgccgctg accgaacccc
     2041 agaaagcggg gatcgcgtca ttctgtccgt acaacattgg tcccggtaag tgtttcccgt
     2101 cgacgtttta cagacgaatt aatgctggtg atcgaaaagg tgcctgcgaa gcgattcgct
     2161 ggtggattaa ggacggtggc agagactgcc gtattcgttc aaacaactgt tacggtcagg
     2221 tatcccgtcg tgaccaggag agcgcgctgg cgtgctgggg tatcgacaga taagcagaat
     2281 attttgctga aaaatgagga atggccacgc gggcggataa cacgaaatcc tgcgaactgg
     2341 cgaaacgtaa gtgaataaaa gtaaaaaccc cgtttgttgg caccaagcga ggttttgtgt
     2401 ttctgacctt gagtaaggca agggagaaca tggcgaagtg taaacgaatt ctgttgaggt
     2461 tgactatgaa aaatggcctt gaactgaaag cgcctgtaac tgatgacatc agcagagcac
     2521 tggcttttgc cattaagtgg gtggcggtcg gtgttgctgt gtccccgatg ctgtatgggc
     2581 tggcaaaact ggtcattgcg ttgaaatcgt gaagggagga ttaagcatgt cagacaaact
     2641 cataacgctg acgaagatcc tctgtgtaat tgtcggcatt tcattttcac taatgctggt
     2701 tgctcttttt ctttccatgg cctggatgat gttgtcttcg tcggggttgc tggggtgaac
     2761 ataaaccgaa tgctttccgc gtttatcgtt attctgctgg tggcctgtgg tgcgctgtgg
     2821 atggcaacag accattaccg tgataacgcg attacctaca aagcgcagcg cgataacaaa
     2881 gccagtgaac tgaagctggc gaacgcaacc attactgata tgcaggtgcg ccagcgcgat
     2941 gttgctgcgc tcgatgcaaa atactcgagg gaattagccg atgcgagagc tgaaaatgaa
     3001 actcttcgcg ctgacgttgc cgctggtcgt aagcgcctgc ggatcaacgc cacctgtcca
     3061 ggctccgtgc gtgaagcccc caccacctcc ggcgtggata atgcaaccgg cccccaactg
     3121 gcagacaccg ttacacggga ttatttcacc ctcagagagc ggctgatgac gatgcacaag
     3181 caactggaag gggcacagga ctatatccgc actcagtgcc tgaaataagt tttgttgatg
     3241 cgccgtatcg tcgctatatt ccctcattaa cagagaccgc agcccgacag ggagactcct
     3301 ctgcgcgagt gtgcggggat aattaaaaac gatgcacacc gggtttttac cgcgttaatg
     3361 attcgcgggt ttatcccggt gcgatggtgg aagaaacagg aagctgtatt acagaaagtg
     3421 ctactactgt atcccgatgc gatgtatgta atgtgagtca gataatggca caggatgtgg
     3481 tgatgtggca gtctggaaca caggatatat tgtcagaata agacccgtag gaataaaaat
     3541 gaaaagacgc cttttactac tttttctgtt atctgtcctg gcagtgggat gctcgcagca
     3601 aaaagctgat gagccccggc aattagtgac ggtgtatcca cgatatccgg aatatgctgc
     3661 agcaaattat atcaaggggc tggttgaggt taagttcgat attggtgctg atgggactgt
     3721 gacacggatc gtttttctcc gctcagagcc tcataatttg tttcgtgatg aagtggtgaa
     3781 ggccatggcg aaatggcgat ttgaaaagaa tcgcccctgt cagggagtga agagacaatt
     3841 tatctttacg ccgtcacgtc cttgatgctt ccaggtagag aggggctgga cgcaggagaa
     3901 aaatgaaaga gccagcggtt atatttttgt catggctgac gaggaatgat ggaagaaggc
     3961 gttgtatgcc acacaacgcc tcactgttca tttcttcttt ttctctggtg gaacccgatg
     4021 aataagagtt gcactggttt ccgatgagat ggcgatatac tcgggcaaag tatgctggca
     4081 gttttccaac tggtcaaaaa tacctgctct cgtctgttgc aatgcctgca gcatgcggcg
     4141 gcaatgcgcc ttgcttttac taaccatctt tccttcctct atcagtcgct gcgtgaactc
     4201 atcatgtaat accaggtaaa tgcggatgtt atcggttttg gctacgcagc atagtacaaa
     4261 acggacaggt gcatcccggg acgggggagg cgtcacatgt ccctgtgatg gttgttccgg
     4321 gtaatgcact gtgtggggca taaaaatgtc cgataatttt actttctacc gcagttagtt
     4381 gattcgttgg tcctggtagc acattgggcg aggatttaaa tgccaggcaa ctgaaggatg
     4441 atgttgcaag ggagatagcg agaatatttc tgattttcat ttgatgatgc ctctgtgtga
     4501 aatgacggta aacgacgcac ttgtgccggc acataatagc aagcaccata atagatcaga
     4561 ttcgattctt gctgtaagtg ataattattc tcgttttcgg gtcctttccg tcgatccaac
     4621 aggttacggg gcggcgacct cgcgggtttt cgctatttat gaaaattttc cgggaaccat
     4681 gtccggtttc tcttcaagtt aactatatga aaaataaaaa aagaggtctt ctgtgaaccg
     4741 gacatgcaca aaaaatagac atgtaagccg gacatgaccg gttttgttgt gattgtgaag
     4801 tgagagtttt tgcgaggtga ggagtggcta cgcagactga agttgccagg catttaagtc
     4861 tgaccgatcg ccagcttcgc agattgcaga aattgccggg tgccccgata tcgaataagc
     4921 gagggcaact ggatctggat gcctggcgcg atttttacat atcgtatctg agaagaagta
     4981 aaaacgatgt gcctgatggc gatagcgaag acgactatga ggagaaattg cttattgcca
     5041 gatgggaact gacagcagaa caggctgtta cacagcagtt aaaaaatgag gtgtcaaaag
     5101 gaaaacttat tgataccggg ttctgtattt ttgccctcag caagctggca atggcgttat
     5161 ccagtacgct tgattccatc cctttatcca tgcagcgaca gtttcctgat ttaacaccgc
     5221 gccatcttga ccatctgaaa acccttattg cgaagggggc aaatcagtgt gcgcgggcgg
     5281 gggataaatt accggattta ctcgatgaat atatcagagc aacaactgaa taatatgatg
     5341 agtgctgtca caacagcatt acagcccctg ataagggcat tgccggtgac gccagttgaa
     5401 tgggctgatc aaaattatta tctgcctaaa gaatcttcat atggtgaggg agaatggaaa
     5461 acgctgccgt tccagatcgc catcatgaac agcatgggga atgatcagat ccggactgtt
     5521 aatctgatta aatctgcccg tgttggctat acaaagatgt tgctgggggt ggtcgggtat
     5581 tttattgagc ataaatcccg aaacagtctg ctttttcagc ccacggattc tgccgctgaa
     5641 gattttatga agtctcacgt ggaggcgacg attcgggatg tgccatgtct gaaagacctt
     5701 tttccatggc tggggcgtaa acatcgtgac aataccctca cgctgaaacg cttttcatcg
     5761 ggtgtgggtt tctggtgcct gggcggcgct gccgccaaaa actaccgtga aaaatccgtg
     5821 gacgtggtct gctatgacga actttcctcg ttcgaaccgg atgtcgaaaa agagggttcg
     5881 ccaaccctgc ttggggataa acgtattgag ggctctgtat ggcccaaatc cattcgcggc
     5941 tcgacgccta aaatcaaagg cacctgccag atcgaaaaag cggccaacga gtcggcgcat
     6001 ttcatgcgtt tttatgtgcc ctgcccacac tgtggggagg agcagtatct gaaatttggc
     6061 gatgaatcca cgccttttgg ccttaaatgg gagaaggaca gccccgaaag cgttttctac
     6121 ctctgtgaac atcatggctg cgtgatccat cagtctgagc ttgaccagag caacgggcgg
     6181 tggatctgtg aaaacacggg gatgtggacc cgtgacggtc tgacgttttt cagcgccgcg
     6241 gataatgaaa ttccgccgcc gcgctccatc acgttccata tctggacagc gtacagtccg
     6301 ttcaccacct gggtacagat agtctatgac tggctggatg cactgaaaga tcccaacggc
     6361 ctgaaaacct ttgtgaacac cacgctgggc gagacctggg aagaggccgt gggcgaaaaa
     6421 ctcgatcacc aggtgctgat ggataaggtt gtgcgttaca cggcggcggt gcctgcccgg
     6481 gtggtttatc tgacggcggg cattgactcg cagcgaaacc gttttgagat gtatgtctgg
     6541 ggatgggcac cgggagagga agcttttctg gtggataaaa tcatcattat ggggcgtcct
     6601 gatgaggaag agacgctgtt acgtgtggat gcggcgatca acaaaaaata ccgccatgcg
     6661 gatggcaccg aaatgaccat ttcccgtgtc tgctgggaca ccggggggat cgatggtgaa
     6721 atcgtctacc agaggtcaaa aaaacacggt gttttccgtg tgctgccggt aaaaggcgcg
     6781 tctgtctatg gcaagccggt gatcaccatg ccgaaaaccc gcaatcagcg gggcgtttat
     6841 ctgtgtgaag tggggacgga caccgcaaaa gaaattctct atgcccgtat gaaagccgag
     6901 cccacgcctg cggatgaagc cacgtcgtat gccatccgtt ttcctgatga tccgga
//
LOCUS       DAHHTG010000185         6903 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.188, whole genome shotgun sequence.
ACCESSION   DAHHTG010000185 DAHHTG010000000
VERSION     DAHHTG010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6903)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6903)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6903
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.188"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            6..3935
                     /locus_tag="NBI74_004195"
     CDS             6..3935
                     /locus_tag="NBI74_004195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_109867760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HCD1934439.1"
                     /translation="MNRIYSLKYCHLTQGLIAVSELASRVSSKTGRKLLAIFIVSALS
                     YGAAEYACAAQMDTRNFWIRDYLDLAQNKGAFQPGAYGVKTPLKNGGEFSFPEVTIPD
                     FSPVSAKGATTAIGNAYSVTASHNGTIHHAIKTQTWGQSDYHYVDRVTKGDFAVQRLD
                     KFVVETAGATEHADFNLSAAEALERYGIEFNGKKQIIGFRVGAGATGVTSYGVGQAYN
                     PLLRSASMFQLNWNNMLATNNTGGFYNEVTGGDSGSGFYLYDNQRKKWVILGTTYGKA
                     FSSKDTWAFFSRYDQNTVDTLKNTFTQEVNLNGQKMTVNNKNIAINDKITAIELTKSN
                     KNKDLKFHGGGSIELTDNLNSGTGGLIFDEGQHYSVIGKDKAYKGAGVEIGKDTVVDW
                     SVKGEANDNLHKTGAGTLNVNVAQGNNLKTGDGTVFLNAEKAFNAIYVASGRGTVKLG
                     QADALDKNSDYRGIYFTSRGGTLDLNGFSQSFKKIAATDVGTIITNTSDKTATLSLQN
                     PSRYVYHGSITGNTNIEHTGTQKSADSSLIIDGNINTRNDITVRNSQLRLQGHATSHA
                     IFREGPRHCYVPGVLCDKDYVTDFARLESEANKKNNSAYKTNNQVASFDQPDWETRHF
                     RFKTLNLENSEFTTARNSVVEGDIVASNSTLKLGGDVPVFIDMYDGINITGNGFGFRQ
                     DVREGRSADDGSSSYTGKITLQKGSTLDINNRFIGGIEAHDSKVNVTSPDALLQNSGV
                     FVNSTLSVRDGGHLTAQKGLYSDGRVQIGKNGTLSLSGTPENGADNTWMPVLTYMTEG
                     YDLTGDNATLNISQQAHVSGDVHATSSSSIRIGSENPGSVSSSVSPVLAAGLFSGYNA
                     AYYGAITGGKGNVSMNNGLWQLTGDSDINSLTTRNSRVQSEENGAFRTLTVKTLDATG
                     SDFVLRTDLKDADKISITEKASGSDNTLNVSFMKNPSPGQSLNIPLVSAPAGTSGDIF
                     KAGTRVTGFSRVTPTLRVDTTGGSTKWILDGFRTEADKAAAAKANSFMNAGYKSFMTE
                     VNNLNKRMGELRDTNGDAGAWARIMNGAGSADGGYSDNYTHVQVGFDKKHALDGVDLF
                     TGVTMTYTDSSADSDAFSGKTKSVGGGLYASALFNSGAYIDLIGKYIHHDNDYTGNFA
                     GLGTKHYGTHSWYAGAETGYRYHLTEDTFIEPQAELVYGAVSGKTFRWKDGEMDLSMK
                     NKDFSPLIGRTGIELGKTFRGKDWSVTARAGTSWQFDLLNNGETVLRDASGEKRIKGE
                     KDSRMLFNVGMNAQIKDNMRFGLEFEKSAFGKYNVDNAINANFRYMF"
     gene            4149..4421
                     /locus_tag="NBI74_004196"
     CDS             4149..4421
                     /locus_tag="NBI74_004196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HCD1934440.1"
                     /translation="MEIREGHNKFYINDEQGKQIAEIVFVPTGENLAIIEHTDVDESL
                     KGQGIGKQLVAKFVEKMRREKRKIIPLCPFAKHEFDKTREYDDIRS"
     gene            4649..4945
                     /gene="ghoS"
                     /locus_tag="NBI74_004197"
     CDS             4649..4945
                     /gene="ghoS"
                     /locus_tag="NBI74_004197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HCD1934441.1"
                     /translation="MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEY
                     GIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDIDFDLSIMTVDDYFRQFYK"
     gene            4973..5146
                     /gene="ghoT"
                     /locus_tag="NBI74_004198"
     CDS             4973..5146
                     /gene="ghoT"
                     /locus_tag="NBI74_004198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system toxin GhoT"
                     /protein_id="HCD1934442.1"
                     /translation="MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITW
                     PMSLPVALLFSLF"
     gene            complement(5265..6782)
                     /gene="lysS"
                     /locus_tag="NBI74_004199"
     CDS             complement(5265..6782)
                     /gene="lysS"
                     /locus_tag="NBI74_004199"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HCD1934443.1"
                     /translation="MSERETRGANEAIDFNDELRNRREKLAALRQQGVAFPNDFRRDH
                     TSDQLHEEFDAKDNQELESLNIEVSVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARD
                     SLPEGVYNDQFKKWDLGDIIGARGTLFKTQTGELSIHCTELRLLTKALRPLPDKFHGL
                     QDQEVRYRQRYLDLIANDKSRQTFVVRSKILAAIRQFMVARGFMEVETPMMQVIPGGA
                     SARPFITHHNALDLDMYLRIAPELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFT
                     MMELYMAYADYHDLIELTESLFRTLAQEVLGTTKVTYGEHVFDFGKPFEKLTMREAIK
                     KYRPETDMADLDNFDAAKALAESIGITVEKSWGLGRIVTEIFDEVAEAHLIQPTFITE
                     YPAEVSPLARRNDVNPEITDRFEFFIGGREIGNGFSELNDAEDQAERFQEQVNAKAAG
                     DDEAMFYDEDYVTALEYGLPPTAGLGIGIDRMIMLFTNSHTIRDVILFPAMRPQK"
BASE COUNT         1952 a         1514 c         1671 g         1766 t
ORIGIN      
        1 tacccgtgaa cagaatatat tcacttaagt attgtcacct tactcaaggg cttatagctg
       61 tatcagaatt agcatcccga gtatcctcta aaaccggtcg aaaacttctc gctatattta
      121 tagtctctgc cttgtcttat ggtgcggctg aatatgcctg tgcagcacag atggacaccc
      181 gaaatttctg gatccgtgat tatcttgatc tggcacagaa taaaggtgca tttcagcccg
      241 gagcatacgg ggtaaaaacg ccattaaaaa atgggggaga attcagtttt cctgaagtaa
      301 caatccctga tttttctcct gtatccgcta aaggtgcaac aactgctatt ggtaatgcct
      361 acagtgttac agcaagtcat aatggcacta ttcaccatgc cattaaaact cagacatggg
      421 gacagtcaga ttatcattat gttgatcgtg tgaccaaagg tgactttgcg gtccagcgtc
      481 tggataagtt tgttgttgaa acagcgggtg caacagagca tgctgatttc aacttatcag
      541 cagcagaagc actagagcgt tatggtattg aattcaatgg aaagaaacaa ataatcggtt
      601 tccgcgttgg agccggagca actggtgtta catcttatgg ggtgggacag gcatataatc
      661 cattattacg cagtgcctct atgtttcagt taaactggaa caatatgtta gccacgaata
      721 atacaggtgg attttataat gaagtgacag gaggagacag cggttccgga ttttatcttt
      781 atgacaatca gagaaaaaaa tgggtcattc ttggaacaac atatggtaaa gcattctcca
      841 gtaaggatac ctgggccttt ttttctcgat atgatcagaa cactgtcgat accttgaaaa
      901 atacttttac ccaagaggtg aacctcaatg gtcagaaaat gacagttaat aataaaaata
      961 ttgccattaa cgataaaata actgctattg aactgaccaa gagtaataaa aataaagatt
     1021 tgaaatttca tggtggcggg agcattgagc tcaccgataa cctgaactca ggaaccggag
     1081 gattgatttt tgatgaggga caacattatt cggttattgg gaaagataaa gcctataaag
     1141 gggcgggtgt tgagatcgga aaagatacgg ttgtcgactg gtcggtaaaa ggggaggcaa
     1201 acgataacct gcacaaaaca ggggccggga cactgaatgt caatgtggcc caggggaata
     1261 acctgaaaac aggtgacggt accgtttttc ttaatgcaga aaaggctttc aatgctatct
     1321 atgttgccag tggccgtgga acggtcaaac tggggcaggc cgatgcgctg gataaaaata
     1381 gtgattacag aggtatttat tttactagtc gtggggggac tctggattta aacgggttca
     1441 gccagtcgtt caagaagatc gcggcaactg atgttggtac cattatcacc aatacttctg
     1501 ataaaacagc gaccctttcc ctacaaaacc cctcccgcta tgtctatcac ggtagtatca
     1561 cgggaaatac gaatatcgaa cacactggga cacagaaaag tgctgacagc agtctgatta
     1621 ttgatggaaa cattaataca cgcaatgaca ttactgtgcg gaattcccag ctcagacttc
     1681 aggggcatgc cacatcacat gcaatattcc gcgaggggcc tcggcactgc tatgtccccg
     1741 gagttctttg tgacaaagat tatgttactg attttgccag actggaaagt gaggcaaata
     1801 agaaaaataa cagtgcctat aaaacaaata atcaggtggc ttcttttgac caacctgact
     1861 gggaaacccg gcatttccga tttaagactc tgaatctgga aaactcagaa ttcacaactg
     1921 cacgtaactc agttgttgag ggtgatattg tcgcatcgaa ttcaacgctg aaactggggg
     1981 gcgacgttcc ggtgttcatt gatatgtatg atggcatcaa tatcaccggt aatggttttg
     2041 gcttccgcca ggacgttcgt gaaggacgct cagcagatga tggcagtagc agctatacag
     2101 gcaaaattac actgcaaaaa ggctccacgc tggacatcaa caaccggttc attggcggta
     2161 ttgaagccca tgacagtaag gtaaacgtca cctcaccgga tgccctgctg cagaacagtg
     2221 gtgttttcgt gaattccacc ctttctgtcc gtgacggcgg tcatctgacg gcacaaaaag
     2281 ggctctacag tgacggccgg gttcagattg gaaagaacgg tacgctttcc ctgagcggca
     2341 cgccggaaaa tggcgctgat aatacctgga tgcccgtact gacatacatg acagaaggct
     2401 atgatttaac cggtgataac gccacgctga acatcagcca gcaggcgcat gtttccgggg
     2461 atgttcatgc aaccagttca tccagcattc gtattggctc cgaaaaccct ggctcagttt
     2521 cctcttctgt ctcccctgtt ctggctgccg ggttgttcag cggatataac gcggcgtact
     2581 acggtgccat caccggcggt aaggggaacg tcagtatgaa taatggcctg tggcagctga
     2641 ccggagattc cgacatcaac agtctgacga cccgtaacag ccgggtacag tctgaagaaa
     2701 acggtgcctt ccgtaccctg acggttaaga cacttgatgc cacgggcagt gattttgtcc
     2761 tgcgcactga tctgaaggac gctgataaaa tcagtattac ggagaaagcc agcggttcag
     2821 acaacaccct gaatgtcagc tttatgaaga acccgtctcc gggacagtcc ctgaatatcc
     2881 cgctggtcag tgcaccggcc ggaacatcag gggatatctt taaggccggc acccgggtga
     2941 caggcttcag tcgtgtgacg ccgacgctgc gtgttgacac cactggcggc agtacgaagt
     3001 ggattctgga tggtttcagg acggaagctg ataaagcggc tgcagcgaag gcgaacagtt
     3061 tcatgaatgc cggctacaaa agctttatga cggaagtaaa caatctgaac aaacgtatgg
     3121 gggagctgcg tgacacgaac ggtgatgccg gtgcctgggc ccgtattatg aacggcgccg
     3181 gttcagccga tggcggatac agtgataact acacccacgt tcaggtcggt tttgacaaaa
     3241 aacatgcgct ggacggtgtt gacctgttca ccggtgtcac gatgacctat accgacagca
     3301 gtgcagacag tgatgcgttc agcgggaaga caaaatccgt ggggggaggt ctgtatgctt
     3361 cagcactgtt taactccggt gcctacattg atttgattgg taaatatatt caccatgaca
     3421 atgactacac aggtaacttt gccggtctgg gtacgaagca ctacggaacc cactcctggt
     3481 atgccggagc ggaaacgggt taccgttatc acctgacgga agacacattt attgagcctc
     3541 aggccgaact ggtttacggc gcagtgtccg ggaaaacatt ccgctggaaa gacggtgaga
     3601 tggacctgag tatgaagaac aaggatttca gcccgttgat tggcagaaca gggattgagc
     3661 tgggcaaaac cttcaggggt aaggactgga gtgtgacagc ccgtgccgga accagctggc
     3721 agtttgacct gctgaataat ggcgagacag ttcttcgtga tgcatccgga gagaaacgga
     3781 tcaaagggga gaaagacagc aggatgttgt tcaatgttgg catgaacgca cagataaagg
     3841 acaacatgcg ctttggtctg gagtttgaga aatccgcctt tggtaaatac aacgtggata
     3901 acgcaataaa cgcgaatttc cggtatatgt tctgacaagg agaggcccct gaagaggggc
     3961 ctgtagtgat gttactcccg ggttaatgca gctagtaaag gtatggctct gactaccctg
     4021 gaaagtggtg cccatcgcat cactacaaat tctggcaatt gcgtacgtgg taacggcaag
     4081 ttatttaacc tcaaacgtac gtgcccgagc ggtgcgctga aataccgtca taaataagcg
     4141 agggtaaaat ggaaatacgc gaaggccaca ataaatttta cattaatgac gaacaaggca
     4201 agcaaatcgc tgaaattgtc tttgtgccga ccggagagaa tttagcgatt atcgaacata
     4261 ccgatgtcga tgaaagcctg aaaggacaag ggattggtaa acagctggtt gcgaaattcg
     4321 tggaaaaaat gcgtcgggaa aaacgaaaaa ttatcccact atgcccattt gcgaaacacg
     4381 aatttgataa aacacgggag tatgatgata ttcgcagttg atgggatagt acagagtcac
     4441 gatatttttc atgctctccg cggtgtgatg caggagagca tctgaagggt aggggatgca
     4501 caaagaatgg tcagagagag cgtttttttg tcccaagtca tcccctttac tgagcaaaaa
     4561 aaaaagaata tctcctatat gagaatcatc attcggggtt aataagtttt gcgtccccag
     4621 agcgttcaat attgatagga gtcatattat ggaaggtaaa aacaagttca atacttatgt
     4681 tgtttctttt gattatccat catcttattc ctccgtgttc ttaaggttaa gatcattgat
     4741 gtatgatatg aatttctcct ctatcgtggc tgatgaatat gggataccac gacaattgaa
     4801 tgaaaactcc ttcgcaataa cgacatcgtt agccgcaagt gaaatcgaag atttaatcag
     4861 gctcaaatgc ttagacttac cggatattga ttttgacctc agcattatga cagttgatga
     4921 ctatttccgt cagttttaca agtagccagg aaggataaga agaacataaa aaatggcact
     4981 attctctaaa atattaattt tttatgtgat tggtgtgaac atatcctttg tcattatctg
     5041 gtttatctca catgagaaaa cacatattcg tttacttagt gcatttctgg tcggaataac
     5101 ctggccaatg agtctgcctg tggcattact tttttctctc ttttaggagc tgtagttgag
     5161 tggacccgct ggttatcttg aaacgaagcg tctctcctca aaactggatt tgcggaatga
     5221 cgaacgaagc agtcgcctga ctgcttcgtt cattaaagtg aaatttattt ctgtgggcgc
     5281 atcgccggga agagaataac gtcgcgaata gtatgactgt ttgtaaacag cataatcatt
     5341 cggtcgatac caatacccag accagcggtt ggcggcagac catattccag cgcagtcacg
     5401 taatcttcgt catagaacat ggcttcgtcg tcacctgcag ctttagcgtt aacctgttcc
     5461 tggaaacgtt cagcctgatc ttctgcgtcg tttaattcgc taaaaccatt accaatttca
     5521 cgaccaccga tgaagaattc aaaacggtcg gtgatttccg ggttaacatc attacggcgc
     5581 gccagcgggg acacttctgc cggatattcc gtaataaagg ttggctgaat cagatgtgct
     5641 tctgccactt catcaaagat ctccgtaaca atacgcccca gtccccagct tttctctacc
     5701 gtaataccga tagattcagc taatgcttta gcagcatcaa aattatccag atccgccata
     5761 tcggtttctg gacgatattt tttgattgct tcgcgcatgg tgagtttttc aaacggtttg
     5821 ccgaaatcaa acacatgctc gccataagtg actttagtgg tacccagtac ctcttgtgcc
     5881 agggtgcgga acagtgactc cgtcagttca atcaaatcat ggtaatccgc atatgccatg
     5941 tagagttcca tcattgtgaa ctcaggatta tggcgaacag aaataccttc attacggaag
     6001 ttacggttga tttcgaatac ccgttcaaaa ccgcctacaa ccagacgttt cagatacagc
     6061 tccggcgcga tacgcaggta catatctaaa tccagagcgt tatgatgggt aataaacggg
     6121 cgagcagatg ccccacctgg aattacctgc atcatcgggg tttctacttc cataaagccg
     6181 cgcgcgacca tgaactgacg gatagcggcc agaatttttg aacggacaac aaacgtttga
     6241 cgggatttat cgttagcgat gaggtccaga taacgttgac gataacggac ttcctgatcc
     6301 tgcagaccat ggaatttatc tggtaaagga cgtagtgctt tagtcagcag gcgcagctca
     6361 gtacagtgaa tggaaagctc acccgtttgc gtcttaaaca gcgtaccgcg ggcaccgata
     6421 atgtcaccca gatcccattt tttaaactga tcgttataaa caccttctgg caggctatct
     6481 ctcgcaacgt acagttgaat acggccaccg acatcctgca acgttacaaa ggaggctttc
     6541 cccataatac gacgggtcat cattcggcca gcaaccgaga cttcaatgtt taaggattcc
     6601 agttcctggt tatccttcgc atcaaactct tcgtgcaact ggtcagaggt atggtcgcgg
     6661 cgaaaatcat tgggaaacgc cacaccttgc tgacgtagtg ctgccagttt ttcgcggcga
     6721 tttctcagtt catcgttaaa atcaatagcc tcattggctc cccgtgtttc tcgttcagac
     6781 attttggttc ctctaaatcc agctttcaat ttagcgtaga taaagagaca gatcggtaca
     6841 ctgtattatc tgcctcgact attaataact cagtttatct tatcgaaact aacgcttacc
     6901 tca
//
LOCUS       DAHHTG010000186         6805 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.189, whole genome shotgun sequence.
ACCESSION   DAHHTG010000186 DAHHTG010000000
VERSION     DAHHTG010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6805)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6805)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6805
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.189"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            complement(36..311)
                     /locus_tag="NBI74_004200"
     CDS             complement(36..311)
                     /locus_tag="NBI74_004200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HCD1934444.1"
                     /translation="MVKKPSQQALNRAAVTVEQAEALAQRLADKPYGAPEKPEPEKQC
                     RTTISLGESMLVTIEDLALRNKRNGKDPKNVSAIVRVALEQYLKTLT"
     gene            complement(305..949)
                     /locus_tag="NBI74_004201"
     CDS             complement(305..949)
                     /locus_tag="NBI74_004201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000633910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCD1934445.1"
                     /translation="MITVVGGNKGGSGKTTIASNLAIALANKGREVCLLNGDLQRTAA
                     KHHAEREAAGLLPAITLVEKFDNLTQTLQALNEKFDDVIVDVAGRNSKEFITSGVVAH
                     QIIAPLQCSQPDLDTLTELEQQIDAMRNLNPELKVYCLQSMATTNPVLRGNERKEFLE
                     YLEEFPTIQVLDSVICFRKVYRDCMSNGTGVVETNNTAAKAEIEHLMNEVFGPW"
     gene            1178..2149
                     /locus_tag="NBI74_004202"
     CDS             1178..2149
                     /locus_tag="NBI74_004202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001505082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid segregation protein parM"
                     /protein_id="HCD1934446.1"
                     /translation="MNVYCDDGSTTIKLAWNDNGKICKSLSQNSFRHGWKVDGLGIRQ
                     TFNYELDGKKYTYDEVSNQSILTTHIEYQYTDVNLLAVHHALLNSGLAPQPVSLTVTL
                     PISEFYTKECQKNELNIQRKIENLMRPIRLNKGDVFTIEHVDVMPESLPAVFSRLVVD
                     KVGQFEKSLVVDIGGTTLDVGVIVGQFDSVSAIHGNSGIGVSSVTKAAMSALRMASSD
                     TSFLVADELIKRRNDPDFVRQVINDETKTDLVLNTIEGAIASLGEQVVNELGDFHHVN
                     RVYVVGGGAPLIYDSIKTAWHHLGQKVVMMESPQTALVEAIAAFKEE"
     gene            2154..2546
                     /locus_tag="NBI74_004203"
     CDS             2154..2546
                     /locus_tag="NBI74_004203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001555635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid partitioning/stability family protein"
                     /protein_id="HCD1934447.1"
                     /translation="MDDERKRKKYTLYLHPEKAADFQTLEAIESVPRSERGELFRNAF
                     ISGMALHQLDPRLPVLLTAILSEEFSADQVVTLLSQTTGWKPSQADIRTVLTELGASQ
                     SVEKMPPSATDSVQEAMNDVRLKMKKLF"
     gene            complement(2551..2802)
                     /locus_tag="NBI74_004204"
                     /pseudo
     CDS             complement(2551..2802)
                     /locus_tag="NBI74_004204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001766293.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4113 domain-containing protein"
     gene            complement(2864..3661)
                     /gene="istB"
                     /locus_tag="NBI74_004205"
     CDS             complement(2864..3661)
                     /gene="istB"
                     /locus_tag="NBI74_004205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000544830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element IS21 family helper ATPase
                     IstB"
                     /protein_id="HCD1934448.1"
                     /translation="MHELEVLLSRLKMEHLSYHVESLLEQAAKKELNYREFLCMALQQ
                     EWNGRHQRGMESRLKQARLPWVKTLEQFDFTFQPGIDRKVVRELAGLAFVERSENVIL
                     LGPPGVGKTHLAIALGVKAVDAGHRVLFMPLDRLIATLMKAKQENRLERQLQQLSYAR
                     VLILDEIGYLPMNREEASLFFRLLNRRYEKASIILTSNKGFADWGEMFGDHVLATAIL
                     DRLLHHSTTLNIKGESYRLKEKRKAGVLTKNTTPISDDEMVKSGQHQ"
     gene            complement(3661..4833)
                     /gene="istA"
                     /locus_tag="NBI74_004206"
     CDS             complement(3661..4833)
                     /gene="istA"
                     /locus_tag="NBI74_004206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000952428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element IS21 family transposase"
                     /protein_id="HCD1934449.1"
                     /translation="MLSREDFYMIKQMRQQGAYIVDIATQIGCSERTVRRYLKYPEPP
                     ARKTRHKMVKLKPFMDYIDMRLAENVWNSEVIFAEIKAMGYTGGRSMLRYYIQPKRKM
                     RPSKRTVRFETQPGYQLQHDWGEVEVEVAGQRCKVNFAVNTLGFSRRFHVFAAPKQDA
                     EHTYESLVRAFRYFGGCVKTVLVDNQKAAVLKNNNGKVVFNSGFLLLADHYNFLPRAC
                     RPRRARTKGKVERMVKYLKENFFVRYRRFDSFTHVNQQLEQWIADVADKRELRQFKET
                     PEQRFALEQEHLQPLPDTDFDTSYFDIRHVSRDSYIEVGGNRYSVPEALCGQPVSIRI
                     SLDDELRIYSNEKLVASHRLCSASSGWQTVPEHHAPLWQQVSQVEHRPLSAYEELL"
     gene            complement(4935..5956)
                     /gene="umuC"
                     /locus_tag="NBI74_004207"
                     /pseudo
     CDS             complement(4935..5956)
                     /gene="umuC"
                     /locus_tag="NBI74_004207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001744069.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V subunit
                     UmuC"
     gene            complement(5956..6393)
                     /gene="umuD"
                     /locus_tag="NBI74_004208"
     CDS             complement(5956..6393)
                     /gene="umuD"
                     /locus_tag="NBI74_004208"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HCD1934450.1"
                     /translation="MSTVYHRPADPSGDDSYVRPLFADRCQAGFPSPATDYAEQELDL
                     NSYCISRPAATFFLRTSGESMNQAGVQNGDLLVVDRAEKPQHGDIVIAEIDGEFTVKR
                     LLLRPRPALEPVSDSPEFRTLYPENICIFGIVTHVIHRTRELR"
     gene            complement(6390..6638)
                     /locus_tag="NBI74_004209"
     CDS             complement(6390..6638)
                     /locus_tag="NBI74_004209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001697964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HCD1934451.1"
                     /translation="MIRIEILFDRQSTKNLKSGTLQALQNEIEQRLKPHYPEIWLRID
                     QGSAPSVSVTGARNDKGKERIQSLLEEIWQDDSWLPAA"
BASE COUNT         1574 a         1653 c         1621 g         1957 t
ORIGIN      
        1 caagtgtgat ttcataagta ataacttatt gattattacg ttagtgtttt taagtattgc
       61 tcaagggcta ctctgacgat ggcacttaca ttttttggat ctttaccatt gcgtttattc
      121 ctcaatgcca gatcttctat tgtgacaagc atgctttcac caagagagat tgtcgtccga
      181 cattgttttt ctggttcggg tttttccggc gcgccgtatg gtttatccgc aaggcgctga
      241 gccagagcct ctgcttgttc taccgtaact gcagctctgt ttaatgcttg ctggctgggt
      301 ttcttcacca tgggccaaat acctcattca ttaaatgttc aatttctgct ttggctgctg
      361 tattgttcgt ttctactaca ccggtgccgt tgctcatgca atcgcgatag acttttctga
      421 aacaaataac tgaatcaagg acttgaattg ttgggaattc ttcaagatat tcaaggaatt
      481 cttttctctc atttcccctc aaaaccggat tggttgttgc catactttgt aaacaataaa
      541 ccttaagttc cgggttaagg ttacgcattg cgtctatctg ttgttctagt tctgtaagag
      601 tatccagatc tggttgagaa cactgtagtg gcgcaattat ttgatgtgcg acaactccac
      661 tcgttatgaa ttcctttgag tttcttcctg cgacatccac tattacatca tcgaactttt
      721 cattcagagc ctgtagtgtt tgtgtcaggt tatcaaattt ctcaacaagg gttattgctg
      781 gtaacagtcc tgcagcttct cgttccgcat gatgttttgc ggctgttcgt tgtagatctc
      841 cgtttaacag gcagacttcc cttcctttat ttgcgagagc aatagccagg tttgatgcaa
      901 tggtagtttt ccctgaacca cctttgttcc caccaactac agtaatcatt gcaacctcat
      961 aaatgtgatg tgtgaagtat gatgatattt tgacacggta acctgaggtg tcaatatgtg
     1021 ttcatattta tgaagtatga aatgtgaagt gtgccatgtg atgtaataaa taatacttgt
     1081 gttttgtgat ttcataacta tgatggtgct caaattgagt attaccttgc aaccagaaac
     1141 aatgatttac tcagattgag tatttaaggg gctatagatg aacgtatact gcgatgatgg
     1201 ttcaacaaca atcaagttgg catggaatga taatgggaaa atctgtaagt cgttgtcgca
     1261 aaactcattt cgtcatggat ggaaggttga cgggttagga attcgccaga catttaacta
     1321 tgaactggat ggtaaaaaat acacgtatga tgaagttagc aaccaatcta ttcttacgac
     1381 tcatattgag tatcaatata cggatgttaa cctgttagct gtgcatcacg ctttactgaa
     1441 tagtggatta gcgcctcagc ctgtatcatt gactgtgact ttgcctatca gtgagttcta
     1501 tacgaaagag tgtcaaaaaa atgaactcaa cattcagcgt aaaattgaga atttaatgag
     1561 gccgattcgt cttaataaag gcgatgtttt caccattgaa cacgttgatg tgatgcctga
     1621 atcattgcca gctgtgtttt ctcgtctggt cgtggataaa gttggtcagt tcgaaaaatc
     1681 gctggtagtt gatattggcg gcactacgct tgatgttggt gttatcgttg gtcaatttga
     1741 ctctgtaagt gccattcatg gtaattctgg cattggtgtg tcttcagtta cgaaagctgc
     1801 aatgagtgca ttgcgcatgg catccagtga taccagtttc ctcgttgcag atgaattgat
     1861 taaacgtcgt aatgacccgg actttgttcg gcaggttata aatgacgaaa caaaaactga
     1921 tctggtcctt aataccattg agggggctat tgccagtttg ggggagcaag tagtcaatga
     1981 gctcggtgat ttccatcatg tgaatcgtgt ttatgttgtc ggtggtgggg ccccgttgat
     2041 ctatgattcg ataaaaacgg catggcacca tcttgggcaa aaagtagtga tgatggagtc
     2101 gccacagaca gcactggttg aagctatcgc ggctttcaaa gaggagtaat attgtggacg
     2161 atgaacggaa aaggaaaaaa tacacacttt atctgcatcc ggaaaaagct gcagactttc
     2221 aaactttgga agcgatagag tccgttccgc gttctgagcg tggtgagctg ttccggaatg
     2281 catttatttc tggaatggcg ttacaccaac ttgatcctcg cctgcctgta ttgctgacag
     2341 ccattttgag cgaggagttt tcagcagatc aggttgtaac cttactcagt cagacgactg
     2401 gatggaagcc ttctcaggcc gatatcagaa ctgtgttgac tgaactcggt gcttcgcaat
     2461 ctgttgaaaa aatgcctcct tctgctactg attcggtgca ggaggcaatg aatgatgtgc
     2521 gtcttaaaat gaaaaaatta ttctgatata tcagaatgaa gcaatgggga tctcagtcca
     2581 ttgtgttgta tacgaaggcg atagcatttc ccgtttcatt tgccattcag gggcggttcc
     2641 tcgccccgca aaccacactt tcccttttcc cgactggttc agttcatcga gtgttttcat
     2701 taacttttca ctgtttttac ggggctggat ttcatcaaat aatcccggct gcgctatacc
     2761 cgatggtgtg aaatcagcca gcatgactcc tgccttcata tagtgtcaac gccacgatgt
     2821 ttgaccgtta tttgccattt tcatgcccgc taagatactt tcgttactga tgctgtccgc
     2881 ttttcaccat ttcatcatca ctgattggcg ttgtgttttt ggtcagcact ccagctttac
     2941 gtttctcttt taaccggtaa ctctctcctt tgatattcag cgtggttgag tgatgtagca
     3001 accgatccag tatcgctgtt gccagcacgt gatctccgaa catttctccc cagtctgcga
     3061 accctttgtt tgacgtcagt atgatgctcg ctttttcata tcgacggttc agtagccgga
     3121 agaacaggct ggcttcctct ctgttcatcg gcagatagcc tatttcatcc aggatcaaca
     3181 cccgggcata actcagttgc tgcagctgac gctccagccg gttttcctgt ttcgctttca
     3241 tcagtgtcgc gatcagtctg tccagtggca taaacagtac ccgatgtccc gcatccaccg
     3301 ctttcacgcc aagagctatg gccagatgag tttttccgac accaggaggt cccagcagga
     3361 tcacgttttc gctgcgctcc acgaacgcca gaccagccag ttcccggaca accttacggt
     3421 cgatgcccgg ctggaaggta aagtcgaact gctccagcgt tttgacccac ggcagacgag
     3481 cctgcttcag cctggactcc ataccgcgct gatgcctgcc gttccattcc tgctgtagcg
     3541 ccatgcacag gaactcccgg tagttcagct cttttttagc tgcctgttcc agcaggcttt
     3601 caacgtgata actcagatgc tccattttca ggcgactcag caggacttcc agctcatgca
     3661 tcacaacagc tcctcatagg cactcagtgg tcgatgttcc acctgactga cctgctgcca
     3721 gagcggggcg tgatgctccg gcactgtctg ccagccagac gatgccgaac agaggcgatg
     3781 tgaggccacc agtttctcat tactgtagat ccgcaactcg tcatccagcg atattcgtat
     3841 cgataccggc tgaccacaca gcgcttcggg aacgctgtaa cgattaccac caacctcgat
     3901 atagctgtcc cgggacacat ggcggatgtc gaagtaactg gtatcgaagt ccgtatccgg
     3961 taacggctgc agatgttcct gctccagcgc gaagcgctgt tccggcgttt ctttgaactg
     4021 gcgaagttcc cgtttgtcag ccacatcggc tatccattgc tccagttgtt gattaacatg
     4081 agtgaagctg tcgaacctgc ggtaccgaac gaagaagttc tccttgaggt atttcaccat
     4141 ccgctcaacc ttaccttttg ttctggccct gcgtggacgg catgcccgtg gcaggaagtt
     4201 atagtggtcg gccagcaaca ggaatccgga gttgaacacg actttcccgt tgttattctt
     4261 cagcaccgca gccttctggt tatcaaccag caccgttttc acacaaccac cgaagtagcg
     4321 gaaggcgcga accagtgatt cgtaggtatg ctcagcatcc tgttttggtg cggcgaagac
     4381 atggaagcgg cgggagaacc ccagcgtatt aaccgcaaag ttaactttgc accgttgccc
     4441 ggcaacctcc acctcaactt cgccccagtc gtgctggagc tggtatccag gctgagtttc
     4501 gaagcgaact gttctttttg acggacgcat tttacgtttg ggctggatgt agtaacgcag
     4561 catggaacgt ccgcccgtat aacccattgc cttaatctcc gcaaagataa cctcactatt
     4621 ccagacattc tctgccaggc gcatgtcgat gtaatccata aacggtttca gcttaaccat
     4681 tttgtggcgg gtctttctgg ctggcggttc agggtatttg aggtagcgtc tgaccgtccg
     4741 ttcagagcaa ccaatctgag tcgcaatatc gacaatgtac gcgccctgct ggcgcatttg
     4801 ctttatcatg taaaagtcct ctctgctcag catgttgatg tcctttctgg tgtgagaacc
     4861 ttaaggaaac aacatgttgg gtggagcgga caatccaaat ggtgaattac cgtcttatat
     4921 cactggcgct gacaatagcg gtacccttca cgccagacat ggtttaagac tctgcatgcg
     4981 gcggcaataa tgtcccggct gtcctgtgtg gcaatgaaag cttttccaca gcggcattgc
     5041 tgtaacaggg ttcttttact gcaaaggggg atgtccgtac aaatgtcgtc acctgccgac
     5101 aatactgacg ctccccacgt agtttctctg cggcccgctc tgcatactga acaacagcct
     5161 ggtgcatggc atctttgtct gtgattcgtt caccaaaact gcgactacag acaatctgct
     5221 gttttgccgg tggtacttct tccagggata tgctggactc gccgttgagt tcgcgtaccg
     5281 tacgctcaag aatgacgctg aagtttttcc ggatgaatgc cgtgttagcc tgcgccagtt
     5341 gcagtgctgt gttaataccc agcgcattca gcttttccgt cagtctgcgt cctactcccc
     5401 agacctcacc aactggctgc agccccagta gcttcaagat ccgattacgg ttttctgccg
     5461 tcagcgcgac cacaccggaa aactgtggcc attgctttgt tgcccactgt gcacttttag
     5521 ccagcgtttt tgtaggcgca atgcccaccc ccatggtgag tcctgtccag ctctttacct
     5581 gttccctgag ctgatgacca aaaaactccg gagagatgca atgatttatc ccccgcaaat
     5641 caatgaacat ttcatcaatt gagtagggct caactgcggg agaaagcgac tccagaacag
     5701 ccataacccg ttggctcatg ctgtggtaca gcgcataatt gctggaaaat acatgtattt
     5761 tcttctccag gcgcatttgt ctcacctgaa accagggctg ccccattctg atgccaaggg
     5821 cttttgcctc cgggctgcgc gcgatcacac agccatcgtt attgctgagt acgatgaccg
     5881 gttcgttgcg aaggtccggg cggaaaactt tttcacatga ggcgtagaaa ctgttgatat
     5941 cagccagtgc aaacatcagc gtaactcccg cgtcctgtgt atcacgtgag tgacaatacc
     6001 aaaaatacag atgttttccg gatacagtgt gcggaactcc gggctgtctg aaaccggctc
     6061 cagtgccggg cgtgggcgca acagcagtcg tttgacggtg aactcaccgt cgatctcagc
     6121 gataacgatg tccccgtgtt gtggtttttc ggccctgtcc actaccagca gatcaccatt
     6181 ctgcacgcca gcctggttca tcgattcacc gctggtgcgc agaaagaagg tggctgcagg
     6241 tctgctgatg caatagctgt tcagatccag ttcctgctca gcataatcag tggcaggcga
     6301 aggaaaaccg gcctggcaac gatcggcaaa caacggtcgc acgtaactgt catcgcctga
     6361 agggtcagcc ggacggtgat aaacggtact catgctgcgg gcagccagct gtcgtcctgc
     6421 cagatttctt ccagcagaga ctggatacgt tctttaccct tgtcgttgcg ggctccagtg
     6481 acagatacag acggggcgct gccctggtct atacgcagcc agatttccgg gtagtgtggt
     6541 ttcagacgtt gttcgatttc attctgtagc gcctgaagtg tgcctgattt gaggtttttt
     6601 gtgctctggc ggtcgaaaag aatttcaatg cggatcatgt tctcatctcc cttttctttt
     6661 gctgtatgta tatgggtctt cccctgttgt ggtgggtggt acataattga gtgattttca
     6721 aacaccacac agtaccttta tggtgtctct ttttacatga gagggaattc ttatgagtgt
     6781 gccgcaaaca aaagctgaac tgctt
//
LOCUS       DAHHTG010000187         6778 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.190, whole genome shotgun sequence.
ACCESSION   DAHHTG010000187 DAHHTG010000000
VERSION     DAHHTG010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6778)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6778)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6778
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.190"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            <1..723
                     /locus_tag="NBI74_004210"
     CDS             <1..723
                     /locus_tag="NBI74_004210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001095581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent DNA methyltransferase"
                     /protein_id="HCD1934452.1"
                     /translation="QMGRYSLDREGLVYAHEGNNGFADLVAEGAYKSFPADSDGILPL
                     MDEEWFDDDVTSRVKEFIRTVWGEEYLRENLDFIAEVLKPKKGESALETIRRYLSTQF
                     WKDHLKMYKKRPIYWLFSSGKEKAFECLVYLHRYNDATLSRMRTEYVVPLLARYQANI
                     DRLNDQLDEASGGEATRLKRERDSLIKKFSELRSYDDRLRHYADMRISIDLDDGVKVN
                     YGKFGDLLADVKAITGNAPEVI"
     gene            900..3497
                     /gene="pglZ"
                     /locus_tag="NBI74_004211"
     CDS             900..3497
                     /gene="pglZ"
                     /locus_tag="NBI74_004211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BREX-1 system phosphatase PglZ type A"
                     /protein_id="HCD1934453.1"
                     /translation="MQNQDFIAGLKAKFAEHRIVFWHDPDKRFIEELEQLKLESVTLI
                     NMTHESQLAVKKRIEIDEPEQQFLLWFPHDAPPHEQDWLLDIRLYSSEFHADFAAITL
                     NTLGIPQLGLREHIQRRKAFFSTKRTQALKNLATEQEDEASLDKKMIAVIAGAKTAKT
                     EDILFNLITQYVNQQIEDDSELENTQAMLKRHGLDSVLWEMLNHEMGYQAEEPSLENL
                     FLKLFCTDLSAQADPQQRAWLEKNVLLTPSGRASALAFMVTWRADRRYKEAYDYCAQQ
                     MQAALHPEDHYRLSSPYDLHECETTLSIEQTIIHALVTQLLEESTTLDREAFKKLLSE
                     RQSKYWCQTQPEYYAIYDALRQAERLLNLRNRHIDGFHYQDSATFWKAYCEELFRFDQ
                     AYRLFNEYALLVHSKGAMILKSLDDYIEALYSNWYLAELSRNWNEVLEAENRMQAWQI
                     PGVPRQQNFFNEVVKPQFQNPQIKRVFVIISDALRYEVAEELGNQINTEKRFTAELRS
                     QLGVLPSYTQLGMAALLPHEQLCYQPGNGDIVYADGLSTSGIPNRDIILKNYKGMAIK
                     SKDLLELKNQEGRDLIRDYEVVYIWHNTIDATGDTASTEDKTFEACRTAVAELKDLVT
                     KVINRLHGTRIFVTADHGFLFQQQALSVQDKTTLQIKPENTIKNHKRFIIGHQLPADD
                     FCWKGKVADTAGVSDNSEFLIPKGIQRFHFSGGARFVHGGTMLQEVCVPVLQIKALQK
                     TAAEKQPQRRPVDIVAYHPMIKLVNNIDKVSLLQTHPVGELYEPRILNIYIVDNANNV
                     VSGKERISFDSDNNTMEKRVREVTLKLIGANFNRRNEYWLILEDAQTETGYQKYPVII
                     DLAFQDDFF"
     gene            3508..5592
                     /gene="brxL"
                     /locus_tag="NBI74_004212"
     CDS             3508..5592
                     /gene="brxL"
                     /locus_tag="NBI74_004212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease Lon-related BREX system protein BrxL"
                     /protein_id="HCD1934454.1"
                     /translation="MQTHHDLPVSGVSAGEIASEGYDLDALLNQHFAGRVVRKDLTKQ
                     LKEGANVPVYVLEYLLGMYCASDDDDVVEQGLQNVKRILADNYVRPDEAEKVKSLIRE
                     RGSYKIIDKVSVKLNQKKDVYEAQLSNLGIKDALVPSQMVKDNEKLLTGGIWCMITVN
                     YFFEEGQKTSPFSLMTLKPIQMPNMDMEEVFDARKHFNRDQWIDVLLRSVGMEPANIE
                     QRTKWHLITRMIPFVENNYNVCELGPRGTGKSHVYKECSPNSLLVSGGQTTVANLFYN
                     MASRQIGLVGMWDVVAFDEVAGITFKDKDGVQIMKDYMASGSFSRGRDSIEGKASMVF
                     VGNINQSVETLVKTSHLLAPFPAAMIDTAFFDRFHAYIPGWEIPKMRPELFTNRYGLI
                     TDYLAEYMREMRKRSFSDAIDKFFKLGNNLNQRDVIAVRRTVSGLLKLMHPDGAYSKE
                     DVRVCLTYAMEVRRRVKEQLKKLGGLEFFDVNFSYIDNETLEEFFVSVPEQGGSELIP
                     AGMPKPGVVHLVTQAESGMTGLYRFETQMTAGNGKHSVSGLGSNTSAKEAIRVGFDYF
                     KGNLNRVSAAAKFSDHEYHLHVVELHNTGPSTATSLAALIALCSILLAKPVQEQMVVL
                     GSMTLGGVINPVQDLAASLQLAFDSGAKRVLLPMSSAMDIPTVPAALFTKFQVSFYSD
                     PVDAVYKALGVN"
     gene            complement(5857..6339)
                     /locus_tag="NBI74_004213"
     CDS             complement(5857..6339)
                     /locus_tag="NBI74_004213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001387059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1934455.1"
                     /translation="MIWQPEFTDKTLSRKPGAVQNGLKTGLGITPGEHIISADSALSR
                     NIRHCFCLSCRGRLILQTDAQGAWFEHDLHALSEQQKAACVVLNPEKSHPYIDDMAMF
                     LSPLPVVLEWHCVMCGQHFPGKKFCEACGTGIYSRAVCTKYVYSYQPDLFQDCGSANT
                     "
     gene            complement(6323..>6778)
                     /locus_tag="NBI74_004214"
     CDS             complement(6323..>6778)
                     /locus_tag="NBI74_004214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HCD1934456.1"
                     /translation="VVYVAFIIDVFAGYIVGWRVSSSMETTFVLDALEQALWVRRPSG
                     TVHHSDKGSQYVSLAYTQRLKEAGLLASTGSTGDSYDNAMAESINGLYKAEVIHRKSW
                     KNRAEVELATLTWVDWYNNRRLLERLGHTPPAEAEKAYYASIGNDDLAA"
BASE COUNT         1753 a         1713 c         1713 g         1599 t
ORIGIN      
        1 caaatgggac gttactccct tgatcgcgaa ggtctggtat acgctcatga aggcaataat
       61 ggcttcgccg atcttgtcgc cgaaggtgct tataaaagct tcccggctga tagtgacggc
      121 attctgccgc taatggatga agagtggttt gacgatgacg tcacctctcg cgtcaaggag
      181 tttatccgca ccgtttgggg cgaagaatat ttgcgcgaaa acctcgattt tatagccgaa
      241 gttctcaagc ccaaaaaagg cgaatctgcg ctggagacca ttcgtcgcta tctttccacc
      301 cagttctgga aagatcatct gaaaatgtat aaaaagcgtc caatctactg gctattcagc
      361 tccggtaaag agaaagcgtt tgagtgcttg gtgtatctgc atcgctataa cgatgccacg
      421 ctgtcgagaa tgcgtaccga atatgtggtg ccgctgctgg cgcgttatca ggccaatatt
      481 gatcgcctga acgatcaact tgatgaggct tctggcggtg aagccacacg tctgaaacgc
      541 gaacgcgaca gcctgatcaa aaaattcagc gaactgcgca gctatgacga tcgcctgcgt
      601 cactatgctg atatgagaat cagtattgat ctcgacgatg gcgttaaggt taactacggc
      661 aagtttggcg atctgctggc cgatgtcaaa gccatcaccg gcaatgcccc agaggtgatc
      721 taaaccagac ggcacgttct cctgttgccg ggttctgccc ggtggcaaat accaccggga
      781 aacgcgccgc tgctgacatt tctccacctc acttcatgat aaaatgcgcc accgtgtcaa
      841 aatctccttt tcgcgttttg gcgctttctt attcatcgta acaacatggg attgtgaact
      901 tgcaaaatca ggactttatt gctggcctta aagctaaatt tgccgaacat cgcatcgttt
      961 tctggcacga tcccgataaa cgttttattg aggaactgga acagctcaag cttgaaagcg
     1021 tcacgctaat caacatgacc cacgagtcac agctggcggt aaaaaaacgc atcgagattg
     1081 atgagccaga acagcagttc ctgctgtggt tcccccatga tgcgccgcct catgaacaag
     1141 actggctgct ggatatccgc ctttacagca gcgaattcca tgccgatttt gccgccatca
     1201 ccctgaacac gctgggcatt ccccagcttg gcctgcgcga gcatattcag cgacgcaagg
     1261 ccttcttcag cactaaacgc acgcaggcgc tgaaaaatct ggcgacagaa caggaagatg
     1321 aagcctcgct ggataagaaa atgattgcgg tgatcgctgg cgcaaagacc gcgaaaaccg
     1381 aagacatttt gttcaacctg attacccagt acgttaacca acaaatagaa gacgacagcg
     1441 aactggaaaa cacgcaggcg atgctgaaac gccacggtct ggactcggta ttgtgggaaa
     1501 tgctcaacca cgaaatgggc taccaggcag aggagccatc gctggaaaac ctgttcctga
     1561 aactgttttg taccgatctc tctgcccagg ccgacccaca gcagcgcgcc tggctggaaa
     1621 aaaatgtcct gctgacgcca tccggcagag catctgccct ggcatttatg gtgacctggc
     1681 gtgccgatcg tcgctataaa gaggcttatg actactgcgc tcagcaaatg caggccgccc
     1741 tgcacccgga agatcattac cgactcagct cgccgtatga tttgcacgaa tgcgaaacca
     1801 ccctcagcat cgaacaaacc attattcatg cgctggtaac acagctgctg gaagagagca
     1861 ccacgctcga tcgggaagcc tttaaaaaac tgctctctga gcgccagagc aaatactggt
     1921 gtcagacaca accagagtat tacgccatct atgacgcatt gcgccaggct gagcggttgc
     1981 tgaacctgcg caatcgccac atcgatggtt tccactacca ggacagcgcc accttctgga
     2041 aagcctactg cgaagaactg ttccgcttcg accaggctta tcgcctgttt aatgaatatg
     2101 ccttgctggt tcacagcaaa ggagcgatga tcctcaagag cctggatgat tatatcgagg
     2161 cgctctacag caactggtat ctggcagagt taagccgtaa ctggaacgaa gtgctggaag
     2221 cggaaaatcg tatgcaggcg tggcaaatcc ctggcgtgcc gcgtcagcag aacttcttca
     2281 atgaggtggt gaagccacag ttccaaaatc cgcaaatcaa acgcgtgttc gtgataattt
     2341 ccgatgccct gcgttatgaa gtggcggagg agctggggaa tcaaatcaat acagagaaac
     2401 gctttaccgc agaactgcgc tcgcagctcg gcgtgctccc cagctacacc caactgggaa
     2461 tggcggcatt gctgccccat gaacaacttt gctatcaacc cggtaacggc gacatcgttt
     2521 atgctgatgg gctgtcgacc tcgggtattc ctaaccgcga tatcattctg aagaactata
     2581 agggaatggc gataaaatcg aaggaccttc tggagttaaa aaatcaggaa gggcgagacc
     2641 ttattcgcga ttacgaagtg gtgtatatct ggcataacac gattgatgcc actggcgaca
     2701 cggcatccac ggaagataaa accttcgaag cgtgccgcac ggcggtggct gaactgaaag
     2761 atttagtcac caaggtgatc aaccgcctcc acggcacacg catttttgtt acggcggatc
     2821 acggtttcct gttccagcaa caggcgcttt cggttcagga taaaaccact ctgcaaatta
     2881 agccggaaaa caccatcaag aaccacaaac gctttattat cggccatcag cttcccgccg
     2941 atgatttttg ctggaaaggg aaagtggcgg ataccgcagg cgtgagcgac aacagcgagt
     3001 tcctgattcc gaaagggatc cagcgcttcc atttctctgg cggcgcgcgc ttcgttcatg
     3061 gcggcaccat gttgcaggag gtttgcgttc cggtattgca gataaaagcc ctgcaaaaaa
     3121 ccgccgcaga aaaacagcca cagcgccgcc cggtggatat tgtcgcttac catccgatga
     3181 ttaagctagt gaacaatatc gataaagtga gcctgttgca gacgcatccg gtgggcgaac
     3241 tttatgaacc gcgtatcctg aacatttaca ttgtcgacaa cgccaacaat gtggtctcgg
     3301 gcaaagagcg catcagcttt gacagtgata acaacaccat ggaaaaacgc gtacgcgaag
     3361 ttacgctgaa gctgattggc gctaacttca accgtcgcaa tgagtactgg ttgatactgg
     3421 aagacgcaca aacggaaacg gggtatcaga agtacccggt cattatcgat ctggcgttcc
     3481 aggatgattt cttctaagtg aggcgatatg caaacccatc atgatttacc tgtttcaggc
     3541 gtatccgcag gggaaattgc ctccgagggt tacgatctgg acgccctgct gaaccagcat
     3601 tttgctggtc gcgtggtgcg taaagatctc accaagcaac tcaaggaagg ggcaaacgtc
     3661 ccggtgtatg tgcttgagta tctgctcggc atgtactgcg cctctgacga tgacgatgtg
     3721 gtcgagcaag ggttgcaaaa cgttaagcgt attctggctg ataactatgt gcgcccggat
     3781 gaagcggaga aagtgaagtc gctgatccgc gagcgtggtt cgtacaaaat catcgataaa
     3841 gtgtcggtga aactgaacca gaaaaaagac gtttacgaag cccagctttc taacctcggc
     3901 atcaaagacg cgctggtgcc ctcgcagatg gttaaagaca acgagaagct actgacgggc
     3961 ggtatctggt gcatgattac cgtcaactat ttctttgaag aagggcagaa gacctcaccc
     4021 ttctcattga tgacgctcaa gcctatccag atgccgaata tggatatgga agaggtgttc
     4081 gatgcgcgta aacactttaa ccgtgaccag tggatcgatg tgctgctgcg ctcggtgggt
     4141 atggagcccg ccaatattga gcaacgcacc aaatggcacc ttatcacccg tatgatcccg
     4201 ttcgtggaga acaactataa cgtttgcgag ctggggccgc gtggcacagg taaaagccat
     4261 gtgtataaag agtgttctcc taactccctg ttagtttccg gcgggcaaac gaccgttgcc
     4321 aacttgttct acaacatggc cagtcgccag atcggcctgg ttggcatgtg ggatgtggta
     4381 gcgttcgacg aagtcgcggg gatcactttc aaagataaag acggcgtgca aatcatgaaa
     4441 gattacatgg cgtcaggatc tttctctcgc ggcagagatt cgattgaagg taaagcgtcg
     4501 atggttttcg tcggcaacat caatcaaagc gtagagactc tcgttaaaac cagccatttg
     4561 ctggcgccat ttccggctgc gatgattgat actgcatttt tcgaccgctt tcatgcctat
     4621 attcccggtt gggaaatccc caaaatgcgc ccggaattat ttaccaaccg ttacgggctg
     4681 attacggatt atctcgctga atatatgcgc gaaatgcgca aacgcagttt ctctgatgcg
     4741 attgataaat tctttaagct gggtaacaac ctcaaccagc gtgacgttat tgccgttcga
     4801 cgtaccgtgt cggggttgtt aaaactcatg catcccgatg gcgcgtacag caaagaagat
     4861 gtgcgagtct gcctgaccta tgcgatggaa gttcgccgcc gcgtgaaaga gcaacttaaa
     4921 aaactgggcg gtctggagtt cttcgatgtg aactttagct acatcgataa cgaaacgctg
     4981 gaagagtttt ttgtaagcgt accggaacag ggcggcagcg aacttattcc tgccggaatg
     5041 ccaaagccgg gtgttgtgca tctggtcact caggcagaaa gcggcatgac cgggctgtat
     5101 cgttttgaaa cacagatgac tgccggtaat ggtaagcata gtgtatcggg tctgggttca
     5161 aatacctccg cgaaagaagc tatccgcgtc ggtttcgatt acttcaaagg caatttgaat
     5221 cgggtaagcg cggccgcgaa attctccgat catgaatatc accttcatgt cgttgaactg
     5281 cataatactg gcccaagcac cgcaaccagt cttgctgcgc ttatcgcttt atgttcgata
     5341 ttgttggcaa aaccggtgca ggaacagatg gtggtgttgg gcagtatgac gcttggtggg
     5401 gtaattaacc cggtgcagga tcttgccgcc agtttgcagc tcgccttcga cagcggtgca
     5461 aaacgggttc tgttgccgat gtcctcggct atggatattc caacggttcc ggcagcgtta
     5521 tttaccaagt ttcaggtgag tttttactca gacccggttg atgctgttta taaggcgctg
     5581 ggtgtgaatt aacgtagtaa ctattttaat gaactggaga ggacaatggc tcaagaacgt
     5641 tctgtaatta aattcatcag accttgcctc tccattcatg aatataaaaa tacttacatc
     5701 tgagtattgt tggctgtgtt tgaataaaaa gagagctgtt caaataagcc tgtataactg
     5761 ttcttttcaa aaaatggagg agctatcagc taattcaaca ctggaagatg ttcgtcgagt
     5821 tggattgaac gccctgcgca gaccaggcat ttcggattaa gtgttcgcac ttccacagtc
     5881 ctggaacaag tctggctggt agctgtaaac atactttgta cataccgctc tggaatagat
     5941 ccccgtgcca caggcttcac agaacttttt cccgggaaaa tgctgcccgc acatcacaca
     6001 atgccactcc agcacgacag gtaatggcga taaaaacatc gccatatcat caatgtaagg
     6061 atgggatttt tccggattca gcaccacaca ggccgccttc tgttgctcgc tcagggcatg
     6121 taaatcatgc tcaaaccacg ccccctgagc atctgtctgc aaaatcaacc gaccacgaca
     6181 ggaaaggcag aaacaatgcc tgatatttct gctgagcgca gagtcggcac tgataatgtg
     6241 ctcacccggc gtgatcccca gccccgtttt tagaccgttc tgaaccgccc cgggtttcct
     6301 ggagagtgtt ttatctgtga actcaggctg ccagatcatc gtttccgatg gaagcataat
     6361 aagctttttc tgcttctgcc ggaggagtat ggcccagcct ttccagcaat cgtcgattgt
     6421 tataccagtc cacccacgtg agtgtggcca gttccacttc tgcacggttt ttccagctct
     6481 tacggtgtat tacctccgct ttgtaaaggc cattgatgct ctccgccatc gcgttgtcat
     6541 acgagtcacc agtacttcct gttgatgcca gtaatccggc ttccttaagc cgctgtgtgt
     6601 aggccagcga tacatactga gaacctttat cactgtgatg gactgtgccg gacggtcgac
     6661 ggacccataa cgcctgctcc agagcatcca acacgaatgt cgtttccata gacgatgaga
     6721 cccgccaccc cacgatgtat ccggcaaaca catcaatgat gaacgccaca tagacgac
//
LOCUS       DAHHTG010000188         6771 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.191, whole genome shotgun sequence.
ACCESSION   DAHHTG010000188 DAHHTG010000000
VERSION     DAHHTG010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6771)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6771)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6771
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.191"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            10..573
                     /locus_tag="NBI74_004215"
     CDS             10..573
                     /locus_tag="NBI74_004215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000915006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like protein"
                     /protein_id="HCD1934457.1"
                     /translation="MLKKTLLSMFATALLSGVAFNALADDPNQGSGKITFKGEVIDAP
                     CSIAPGDEDQTINLGEVADTVLKSGQKSLPVDVTIHLQDCILSDGTNTVDKVKITFSS
                     ASVDATDSNLLKNTLEGNIGGATDVGVRLVKSDNTNVTLGTPITINFPTTNSYQELNF
                     KARMESLGRTATPGNVQAQANYVLDYK"
     gene            658..3180
                     /locus_tag="NBI74_004216"
     CDS             658..3180
                     /locus_tag="NBI74_004216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001060675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HCD1934458.1"
                     /translation="MNNKNTFTGDTLSHAIKNALSGVVCSLLFVLPAHAVEFNVDMID
                     VEDRENIDISRFEKKGYIPPGRYLARVQINKNMLPQALILEWVKADNESGSLLCLTKE
                     DLTSFGLNTEFIESLQTIAGRECLDLSQRQELTTRLDKATMILSLSVPQAWLKYQATN
                     WTPPEFWDTGIAGFILDYNVYASQYAPHHGDSTQNVSSYGTLGFNLGAWRLRSDYQYN
                     QNFADGRSVNRDSEFARTYLFRPIPSWSSKFTMGQYDLSSNLYDTFHFTGASLESDES
                     MLPPDLQGYAPQITGIAQTNAKVTVAQNGRVLYQTTVAPGPFTISDLGQSFQGQLDVT
                     VEEEDGRTSTFQVGSASIPYLTRKGQVRYKTSLGKPTSVGHNDINNPFFWTAEASWGW
                     LNNVSLYGGGMFTADDYQAITTGIGFNLNQFGSLSFDVTGADASLQKQNSDNLRGYSY
                     RFNYAKHFESTGSQITFAGYRFSDKDYVSMSEYLSSRNGDESIDNEKESYVISLNQYF
                     ETLELNSYLNVTRNTYWDSASNTNYSVSVSKNFDIGDFKGISASLAVSRIRWDDDEEN
                     QYYFSFSLPLQQNRNISYSMQRTGSSNTSQMISWYDSSDRNNIWNISASATDDNIRDG
                     EPTLRGSYQHYSPWGRLNINGSVQPNQYNSVTAGWYGSLTATRHGVALHDYSYADNAR
                     MMVDTDGISGVEINSNRTVTNGLGIAVVPSLSNYTTSMLRVNNNDLPEGVDVENSVIR
                     TTLTQGAIGYAKLNATTGYQIVGVIRQENGRFPPLGVNVTDKATGKDVGLVAEDGFVY
                     LSGIQENSILHLTWGDNTCEVTPPNQSNISESAIILPCKTVK"
     gene            3196..3936
                     /locus_tag="NBI74_004217"
     CDS             3196..3936
                     /locus_tag="NBI74_004217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001374399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HCD1934459.1"
                     /translation="MKSPLITTGMICLLGICNFAQATVSPDRTRIIFNASNKSATVRL
                     TNQSKIDPYLAQSWIEDASGKKTRGYISTLPPMERIEPDEQVQIRLMALASLNNLPQD
                     RETLFYYNVREIPPRTKEQNVMQIAMQSRLKLFWRPKAIELKEGQMVPLQKVTITRTA
                     AGLTLNNPTPYHITVGYIGTNGKTLMPGADSIMVTPFASATQYLNNLPSTFQLGFVAD
                     YGGLEMFKVECNSVQSLCQSSPAKKGKI"
     gene            3933..4982
                     /locus_tag="NBI74_004218"
     CDS             3933..4982
                     /locus_tag="NBI74_004218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000631269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein"
                     /protein_id="HCD1934460.1"
                     /translation="MITLFRLLAILCLFYNVSVYAVNCYQDQRGGNTQIRGTLPSFRI
                     PENAQPGQKIWESDDVNVTVYCDNATGWRADDPAENIYAWIKMPAINSADILNNPYLT
                     FGVTYNGVDYEDSDVGIDTFACLDKYEQYYGGIYHDPVCNGSTLQKNVTFNARFRAYV
                     KFKARPAVDQTWDFGVVNVLQFDGEGGANLAADNKNLRYIIDGLNNILFLDCSVDVRI
                     FPESQIVNFGQISANSIATYRPKAAFSVSTIKDVAADCTEQFDVVTSFYTTDTLHDDT
                     HLEMGNGLLMRITDQKTQEDIKFNKYKRFTTYIPGQTGAMVTRDYQAELSQKPGETLV
                     YGPFKKDLIVKINYN"
     gene            complement(5036..5689)
                     /gene="ribB"
                     /locus_tag="NBI74_004219"
     CDS             complement(5036..5689)
                     /gene="ribB"
                     /locus_tag="NBI74_004219"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HCD1934461.1"
                     /translation="MNQTLLSSFGTPFERVENALAALREGRGVMVLDDEDRENEGDMI
                     FPAETMTVEQMALTIRHGSGIVCLCITDDRRKQLDLPMMVENNTSAYGTGFTVTIEAA
                     EGVTTGVSAADRITTVRAAIADGAKPSDLNRPGHVFPLRAQAGGVLTRGGHTEATIDL
                     MTLAGFKPAGVLCELTNDDGTMARAPECIEFANKHNMALVTIEDLVAYRQAHERKAS"
     regulatory      complement(5793..5941)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            6063..6362
                     /gene="ubiK"
                     /locus_tag="NBI74_004220"
     CDS             6063..6362
                     /gene="ubiK"
                     /locus_tag="NBI74_004220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311957.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis accessory factor UbiK"
                     /protein_id="HCD1934462.1"
                     /translation="MIDPKKIEQIARQVHESMPKGIREFGEDVEKKIRQTLQAQLTRL
                     DLVSREEFDVQTQVLLRTREKLALLEQRISELEARNNPAAELQSPPAIPPVDKAE"
     gene            complement(6397..6597)
                     /gene="glgS"
                     /locus_tag="NBI74_004221"
     CDS             complement(6397..6597)
                     /gene="glgS"
                     /locus_tag="NBI74_004221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface composition regulator GlgS"
                     /protein_id="HCD1934463.1"
                     /translation="MDHSLNSLNNFDFLARSFARMHAEGRPVDILAVTGNMDEEHRTW
                     FCARYAWYCQQMMQTRELELEH"
BASE COUNT         1930 a         1600 c         1489 g         1752 t
ORIGIN      
        1 attatagtca tgttaaaaaa aacattgtta tctatgttcg caaccgcatt gttatctggc
       61 gttgctttta acgcgcttgc tgatgaccca aatcagggtt ctggtaaaat aacatttaaa
      121 ggtgaagtta tcgatgcgcc ttgttctatt gctcctggtg atgaagatca gacaataaac
      181 ctcggtgaag ttgctgatac tgtattaaaa agcggacaga aatctcttcc tgtagatgtc
      241 accattcact tgcaggattg tattttatct gacggcacta acactgttga taaagtcaaa
      301 atcaccttta gttctgccag tgttgacgct accgactcca acctgcttaa aaacactctg
      361 gaaggtaaca tcggcggcgc aactgatgta ggcgtacgtc tggtgaaatc agacaacacc
      421 aacgtgactc ttggcactcc aatcactatc aacttcccga cgactaactc ctaccaggag
      481 ttgaacttta aagcccgtat ggaatctctg ggacgcaccg cgaccccggg taacgtgcag
      541 gcacaggcca attacgtact cgactacaag taagcggcaa ttaatacgca caacgtcaga
      601 cggtaaagcc gatgatatgt catcggcaac tttgggtctt ttttgaaatg gatttttatg
      661 aacaataaaa acacgtttac cggggataca ttatcccacg caattaaaaa tgccctgtct
      721 ggcgtagtgt gttcgctact cttcgtttta cctgcccatg cagtggaatt caacgttgat
      781 atgattgatg ttgaagaccg tgagaatatc gacatctctc gttttgagaa aaaaggctat
      841 atccccccag gcagatacct cgctcgtgtg caaataaata aaaatatgtt gccacaagcg
      901 ttaatactgg aatgggtaaa agccgataat gaaagtggtt cgttactctg cttaaccaaa
      961 gaagatctga ctagtttcgg tcttaatacg gaatttattg aatcattgca aaccatagct
     1021 ggcagagaat gtctcgattt aagccaacgt caggagttaa cgacacggct tgataaagct
     1081 acgatgatat tatcgctaag tgttccccag gcgtggttaa aataccaggc aacaaactgg
     1141 acgccaccag agttttggga taccggtatc gccgggttta tccttgatta caatgtgtac
     1201 gccagccaat atgccccaca tcatggagac agcacccaaa acgtcagctc ctatggtacg
     1261 ttaggcttta acctcggcgc atggcgctta cgtagcgatt accaatataa tcagaatttt
     1321 gctgatggac gctcggtaaa ccgcgacagc gaatttgcgc gaacttatct gtttcgccct
     1381 atcccctcct ggtcgtcaaa attcactatg ggccagtacg acctgagctc caatctttac
     1441 gataccttcc actttactgg cgcatcgctg gaaagtgatg aaagcatgct gccgccagat
     1501 ttacagggtt atgcgccaca aattaccggc atcgcgcaga ccaacgcgaa agtaactgtg
     1561 gcacaaaatg gtcgtgtact ttatcaaacc actgtcgcgc caggcccttt tactatttct
     1621 gatttggggc aatcgtttca ggggcagctg gatgtcacag tggaagaaga agatggccgc
     1681 accagcacct tccaggttgg atccgcatcc attccctatt taacccgtaa agggcaagtg
     1741 cgctataaaa cgtcgctggg aaaaccgaca tccgtcgggc ataacgatat caataatccc
     1801 tttttctgga cggcggaagc ctcctggggc tggttgaaca atgtgtcgct gtatggtggc
     1861 ggcatgttca ccgctgatga ttatcaggct atcactaccg gtattggctt taatcttaac
     1921 caattcggtt cgctttcttt tgatgtcacc ggagcagacg cgtctttgca gaaacaaaat
     1981 agcgacaatc tgcgtggtta cagctatcgc ttcaactatg caaagcattt cgaatcgaca
     2041 ggtagtcaga ttaccttcgc gggttatcgc ttctcagata aagattacgt gtcgatgagt
     2101 gagtacctaa gctcgcgtaa tggcgatgag tcaattgata atgaaaaaga gagttatgtc
     2161 atttccttga accagtactt tgaaacactg gaattaaact cttatctcaa cgttacacgc
     2221 aatacttatt gggacagcgc cagcaatacc aactactccg tatctgtaag caaaaacttt
     2281 gatattggcg atttcaaagg tatctctgca tcgctggcag taagtcgaat ccgctgggat
     2341 gacgacgaag agaatcaata ttacttctct ttctctctac ctttacaaca aaaccgcaac
     2401 atctcctaca gtatgcagcg tacgggaagc agtaatactt cgcagatgat ttcctggtac
     2461 gattcatcag atcgcaacaa tatctggaat atttcagcgt cggcaacgga cgacaatata
     2521 cgtgatggcg aaccaacact gcgcggcagc taccagcact attcaccgtg gggacgtctg
     2581 aacattaacg gcagtgtaca gccgaatcag tacaattctg ttaccgcagg ctggtacggt
     2641 tcacttaccg ctacacgtca tggtgtcgcc cttcacgatt atagctatgc tgataacgcc
     2701 cgcatgatgg tcgataccga cggcatctcc ggcgttgaaa tcaactctaa ccgaactgtt
     2761 accaacgggc tgggcattgc cgttgtacct tcgttatcga actacaccac ctccatgttg
     2821 cgggtgaaca ataacgatct gccagaaggt gtcgatgtcg aaaactcggt tattcgtact
     2881 acgctcaccc agggtgccat cggctacgca aaactgaatg ccaccaccgg ataccaaatc
     2941 gtcggcgtta ttcgtcagga aaatggccgc ttccctccac taggtgtgaa tgtcacggat
     3001 aaagcgacag gtaaagatgt gggcctggtc gcggaagacg gcttcgttta tctcagcggt
     3061 attcaggaaa acagtattct gcatttaacc tggggtgata atacctgtga agtcacgccg
     3121 ccaaaccaaa gtaacattag cgaaagcgca ataattttac cttgtaaaac agtcaaataa
     3181 ttcaggatga atataatgaa atcaccatta atcacaacag gaatgatttg tttactgggg
     3241 atctgcaatt ttgcccaggc gactgtctcc cccgacagga cacgtatcat ttttaatgct
     3301 tcgaataaaa gcgcaacggt ccgtctgact aaccaaagta aaatcgatcc ctatctggct
     3361 cagtcgtgga ttgaagatgc cagcggtaaa aaaacgcgtg gttacatatc aacactgccg
     3421 ccgatggaac gtattgagcc ggatgaacag gtacaaattc gcctgatggc gctggcctca
     3481 ttaaacaact taccgcagga cagagaaacg ctgttctact ataacgtgcg ggaaattccg
     3541 cctcgcacta aagaacagaa tgtgatgcaa atcgccatgc agagtcgact gaaattattc
     3601 tggcgtccga aagcaatcga attaaaagaa gggcagatgg tgcctttaca aaaagtcaca
     3661 ataacacgga ccgctgcagg gctgacactc aataatccaa cgccttatca catcaccgtc
     3721 gggtatatcg gtactaacgg caagacactg atgcccggtg cagacagcat tatggtgact
     3781 ccgtttgctt ctgcaactca atacttgaac aacttgccct caactttcca gttaggcttt
     3841 gttgctgact atggtggact ggagatgttt aaagtcgaat gtaattccgt ccaatccctc
     3901 tgccaaagct ctcctgctaa gaagggaaaa atatgattac gcttttccgt ttactggcga
     3961 ttctttgcct tttttataac gtttcagttt atgctgtaaa ttgctatcag gatcaaagag
     4021 gtggtaatac acaaatacgt ggtactttac cttccttcag aattccagaa aacgcccagc
     4081 cgggacaaaa aatttgggaa agcgatgacg ttaatgttac ggtatattgt gataacgcga
     4141 caggatggag agctgatgat cccgcagaaa atatttatgc ctggattaaa atgccggcta
     4201 taaatagtgc agacattttg aataacccat acctgacatt cggtgtaacc tataatggtg
     4261 tggactatga agatagcgat gtaggaattg atactttcgc ctgtctggat aagtatgaac
     4321 agtattatgg tggtatttat catgatccag tttgtaatgg cagcacccta caaaaaaatg
     4381 tcacgtttaa tgcccgtttt cgcgcatatg tgaaatttaa agcacgtcca gcagtggatc
     4441 aaacctggga ttttggcgtg gtaaatgttc tgcaatttga tggtgaaggt ggtgctaact
     4501 tagcagcaga taataaaaat ctacgttata ttattgatgg attaaataat attttatttc
     4561 ttgattgtag tgtcgatgtc cgtatttttc cagaaagcca gatcgttaat ttcgggcaaa
     4621 tctctgcaaa ctctattgct acttatcgac ccaaagctgc atttagcgta tctactataa
     4681 aagatgttgc tgccgactgt accgagcaat ttgatgttgt caccagtttt tatacgacag
     4741 atacgttaca tgatgatacg catctggaaa tgggtaatgg tttgttaatg cgtattactg
     4801 atcaaaaaac acaagaagac attaagttta ataagtacaa acgcttcacc acatatattc
     4861 caggtcagac tggagcgatg gttacccgtg attaccaggc tgaactttca caaaaaccgg
     4921 gggagactct cgtttatggg cctttcaaaa aagatctcat cgtaaaaata aactataact
     4981 gaacagcaaa atgccgggaa caccccggca ttttcatatt tagtatttca cgtcattagc
     5041 tggctttacg ctcatgtgcc tgacggtatg ccaccaggtc ttcaatagtc acgagcgcca
     5101 tattgtgttt attcgcaaac tcaatgcact ctggtgcacg cgccatcgtg ccatcgtcat
     5161 tagtcagctc acacagcaca ccagctggtt taaagcctgc cagcgtcatc agatcgatag
     5221 tcgcttcagt atgaccgcca cgcgtcagta caccacctgc ctgcgcgcga agtgggaaaa
     5281 cgtggcctgg gcgattcaga tctgaaggtt ttgcgccatc ggcaatcgct gcgcgaacgg
     5341 tcgtaatacg gtcagcggca gaaacaccgg tagtcacacc ttcagctgct tcaatggtca
     5401 cggtaaaacc ggtgccatag gcgctggtgt tattttctac catcattggc agatcgagtt
     5461 gtttacggcg atcgtcagta atgcacaggc aaacaatacc actcccgtgg cgaatggtca
     5521 gcgccatctg ctcaacggtc atggtttctg ccgggaagat catatcgcct tcgttttcac
     5581 ggtcttcatc atcaagcacc atcacaccgc gtccttcacg cagcgcagcc agtgcatttt
     5641 caacacgttc gaaaggcgta ccaaaagagg aaagtagcgt ctgattcatg gtaaaaaaac
     5701 ctcactaaaa ttatggttac cagaatcagg gcagtcttag gagtggcggc atatagccaa
     5761 aataacgtga gcgggtccat gcccgacaga atcgttactc tctcccatcc ggactctaac
     5821 cgtcggcccc ggaattacac cggatctgct gtcctttgag ttcgcaccca aagcgctcgc
     5881 gggctttcaa ctgagttgat ttaccgccgg tggggaattt cgccccgccc tgagaataag
     5941 cggattcact ataacgctaa tgattagcgc cagcaacgca tagcttcaca taattctggt
     6001 ttatgactta cccttatcgc actacaatgg cactcaacac ctatcagtac agggaaacta
     6061 caatgattga cccgaaaaaa attgagcaaa tcgctcgcca ggttcacgaa tcaatgccta
     6121 aaggaatcag ggagttcggg gaagatgtgg agaaaaaaat ccgccaaacc ctacaagcgc
     6181 agctgacgcg tctcgatctg gtaagccgcg aagaattcga cgttcagacg caagtcctgt
     6241 tacggactcg tgaaaaactg gcgttactgg agcagcgcat cagcgaactg gaagcgcgca
     6301 ataatccggc agcagaactt caatctcctc cggctatccc gccagttgat aaagcagaat
     6361 aaagcaccac aaaaacgggc cagacggccc gtaatatcag tgttctaatt caagttctct
     6421 ggtttgcatc atctgttggc aataccaggc ataacgtgcg caaaaccagg ttctatgttc
     6481 ttcatccatg ttaccagtaa cggccagaat atcgaccggg cgaccttctg cgtgcattct
     6541 ggcaaaacta cgcgcgagaa aatcgaaatt atttaaagaa ttaagactat gatccataag
     6601 cacatcctcc ggttattgca taccttttaa ggccatatgc tttttctgat ttcagtattc
     6661 cccaattagt gtagtgcctt taaacataac gtgcgtaaat atattgtccc cgatcacact
     6721 ttttagtgaa aaataaaaaa accatattaa aagactttct cttccagaac t
//
LOCUS       DAHHTG010000189         6483 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.192, whole genome shotgun sequence.
ACCESSION   DAHHTG010000189 DAHHTG010000000
VERSION     DAHHTG010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6483)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6483)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6483
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.192"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            73..477
                     /gene="ybgC"
                     /locus_tag="NBI74_004222"
     CDS             73..477
                     /gene="ybgC"
                     /locus_tag="NBI74_004222"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HCD1934464.1"
                     /translation="MNTTLFRWPVRVYYEDTDAGGVVYHASYVAFYERARTEMLRHHH
                     FSQQALMAERVAFVVRKMTVEYYAPARLDDMLEIQTEITSMRGTSLVFTQRIVNAENT
                     LLNEAEVLVVCVDPLKMKPRALPKSIVAEFKQ"
     gene            474..1166
                     /gene="tolQ"
                     /locus_tag="NBI74_004223"
     CDS             474..1166
                     /gene="tolQ"
                     /locus_tag="NBI74_004223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolQ"
                     /protein_id="HCD1934465.1"
                     /translation="MTDMNILDLFLKASLLVKLIMLILIGFSIASWAIIIQRTRILNA
                     AAREAEAFEDKFWSGIELSRLYQESQGKRDNLSGSEQIFYSGFKEFVRLHRANSHAPE
                     AVVEGASRAMRISMNRELENLETHIPFLGTVGSISPYIGLFGTVWGIMHAFIALGAVK
                     QATLQMVAPGIAEALIATAIGLFAAIPAVMAYNRLNQRVNKLELNYDNFMEEFTAILH
                     RQAFTVSESNKG"
     gene            1170..1598
                     /gene="tolR"
                     /locus_tag="NBI74_004224"
     CDS             1170..1598
                     /gene="tolR"
                     /locus_tag="NBI74_004224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin uptake protein TolR"
                     /protein_id="HCD1934466.1"
                     /translation="MARARGRGRRDLKSEINIVPLLDVLLVLLLIFMATAPIITQSVE
                     VDLPDATESQAVSSNDNPPVIVEVSGIGQYTVVVEKDRLERLPPEQVVAEVSSRFKAN
                     PKTVFLIGGAKDVPYDEIIKALNLLHSAGVKSVGLMTQPI"
     gene            1663..2973
                     /gene="tolA"
                     /locus_tag="NBI74_004225"
     CDS             1663..2973
                     /gene="tolA"
                     /locus_tag="NBI74_004225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="HCD1934467.1"
                     /translation="MSKATEQNDKLKRAIIISAVLHVILFAALIWSSFDENIEASAGG
                     GGGSSIDAVMVDSGAVVEQYKRMQSQESSAKRSDEQRKMKEQQAAEELREKQAAEQER
                     LKQLEKERLAAQEQKKQAEEAAKQAELKQKQAEEAAAKAAADAKAKAEADAKAAEEAA
                     KKAAADAKKKAEAEAAKAAVEAQKKAEAAAAALKKKAEAAEAAAAEARKKAATEAAEK
                     AKAEAEKKAAAEKAAADKKAAEKAAAEKAAADKKAAAEKAAADKKAAEKAAAEKAAAD
                     KKAAAEKAAADKKAAAAKAAAEKAAAAKAAAEADDIFGELSSGKNAPKTGGGAKGNNA
                     SPAGSGNTKNNGASGADINNYAGQIKSAIESKFYDASSYAGKTCTLRIKLAPDGMLLD
                     IKPEGGDPALCQAALAAAKLAKIPKPPSQAVYEVFKNAPLDFKP"
     gene            3106..4398
                     /gene="tolB"
                     /locus_tag="NBI74_004226"
     CDS             3106..4398
                     /gene="tolB"
                     /locus_tag="NBI74_004226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001646851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolB"
                     /protein_id="HCD1934468.1"
                     /translation="MKQALRVAFGFLILWASVLHAEVRIVIDSGVDSGRPIGVVPFQW
                     AGPGAAPEDIGGIVAADLRNSGKFNPLDRARLPQQPGSAQEVQPAAWSALGIDAVVVG
                     QVTPNPDGSYNVAYQLVDTGGAPGTVLAQNSYKVNKQWLRYAGHTASDEVFEKLTGIK
                     GAFRTRIAYVVQTNGGQFPYELRVSDYDGYNQFVVHRSPQPLMSPAWSPDGSKLAYVT
                     FESGRSALVIQTLANGAVRQVASFPRHNGAPAFSPDGSKLAFALSKTGSLNLYVMDLA
                     SGQIRQVTDGRSNNTEPTWFPDSQNLAFTSDQAGRPQVYKVNINGGAPQRITWEGSQN
                     QDADVSSDGKFMVMVSSNGGQQHIAKQDLATGGVQVLSSTFLDETPSLAPNGTMVIYS
                     SSQGMGSVLNLVSTDGRFKARLPATDGQVKFPAWSPYL"
     gene            4433..4954
                     /gene="pal"
                     /locus_tag="NBI74_004227"
     CDS             4433..4954
                     /gene="pal"
                     /locus_tag="NBI74_004227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HCD1934469.1"
                     /translation="MQLNKVLKGLMIALPVMAIAACSSNKNASNDGSEGMLGAGTGMD
                     ANGGNGNMSSEEQARLQMQQLQQNNIVYFDLDKYDIRSDFAQMLDAHANFLRSNPSYK
                     VTVEGHADERGTPEYNISLGERRANAVKMYLQGKGVSADQISIVSYGKEKPAVLGHDE
                     AAYAKNRRAVLVY"
     gene            4964..5755
                     /gene="cpoB"
                     /locus_tag="NBI74_004228"
     CDS             4964..5755
                     /gene="cpoB"
                     /locus_tag="NBI74_004228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein CpoB"
                     /protein_id="HCD1934470.1"
                     /translation="MSSNFRHQLLSLSLLVGIAAPWAAFAQAPISSVGSGSVEDRVTQ
                     LERISNAHSQLLTQLQQQLSDNQSDIDSLRGQIQENQYQLNQVVERQKQILLQIDSLS
                     SGGAAAQSTSGDQSGVVASTTPTADAGTANAGAPVKSGDANTDYNAAIALVQDKSRQD
                     DAMVAFQNFIKNYPDSTYLPNANYWLGQLNYNKGKKDDAAYYFASVVKNYPKSPKAAD
                     AMFKVGVIMQDKGDTAKAKAVYQQVISKYPGTDGAKQAQKRLNAM"
     gene            5920..5995
                     /locus_tag="NBI74_004229"
     tRNA            5920..5995
                     /locus_tag="NBI74_004229"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5953..5955,aa:Lys,seq:ttt)
     gene            6031..6106
                     /locus_tag="NBI74_004230"
     tRNA            6031..6106
                     /locus_tag="NBI74_004230"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6064..6066,aa:Val,seq:tac)
     gene            6109..6184
                     /locus_tag="NBI74_004231"
     tRNA            6109..6184
                     /locus_tag="NBI74_004231"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6142..6144,aa:Lys,seq:ttt)
     gene            6236..6311
                     /locus_tag="NBI74_004232"
     tRNA            6236..6311
                     /locus_tag="NBI74_004232"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6269..6271,aa:Val,seq:tac)
     gene            6315..6390
                     /locus_tag="NBI74_004233"
     tRNA            6315..6390
                     /locus_tag="NBI74_004233"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6348..6350,aa:Lys,seq:ttt)
BASE COUNT         1717 a         1515 c         1784 g         1467 t
ORIGIN      
        1 caaacctcaa tcgcgcgcgt atagtagcag cgtttaaaag ctctaacttt tgttgcatta
       61 ccgggatgta aagtgaatac aacgctgttt cgatggccgg ttcgcgtcta ctatgaagat
      121 accgatgccg gtggtgtggt gtaccacgcc agttacgtcg ctttttatga aagagcacgc
      181 acagagatgc tgcgtcatca tcatttcagt caacaggcgc tgatggctga acgcgttgcc
      241 tttgtggtac gtaaaatgac ggtggaatat tacgcacctg cgcggctcga cgatatgctc
      301 gaaatacaga ctgaaataac atcaatgcgt ggcacctctt tggttttcac gcaacgtatt
      361 gtcaacgccg agaatacttt gttgaatgaa gcagaggttc tggttgtttg cgttgaccca
      421 ctcaaaatga agcctcgtgc gcttcccaag tctattgtcg cggagtttaa gcagtgactg
      481 acatgaatat ccttgatttg ttcctgaagg ctagccttct ggttaaactt atcatgttga
      541 ttttgattgg tttttcaatc gcatcctggg ccattattat ccagcggacc cgtattctta
      601 acgcagcggc gcgcgaagcc gaagcgtttg aagataaatt ctggtctgga atcgaactct
      661 ctcgcctcta tcaagagagc caggggaaac gggataatct gagcggttcg gaacaaatct
      721 tttacagcgg cttcaaagag tttgtgcgcc tgcatcgtgc caatagccat gcgccggaag
      781 ccgtagtgga aggggcgtcg cgtgcgatgc gtatttccat gaatcgtgaa cttgaaaatc
      841 tggaaacgca cattccgttc ctcggtacgg ttggctccat cagcccgtat attggtctgt
      901 ttggtacggt ctgggggatc atgcacgcct ttatcgccct cggggcggta aaacaagcaa
      961 cactgcaaat ggttgcgccc ggtatcgcag aagcgttgat tgcgacggca attggtttgt
     1021 tcgccgcaat tccggcggta atggcttata accgtctcaa ccagcgcgta aacaaactgg
     1081 aactgaatta cgacaacttt atggaagagt ttaccgcgat tctgcaccgc caggcgttta
     1141 ccgttagcga gagcaacaag gggtaagcca tggccagagc gcgtggacga ggtcgtcgcg
     1201 atctcaagtc cgaaatcaac attgtaccgt tgctggacgt actgctggtg ctgttgctga
     1261 tctttatggc gacagcgccc atcatcaccc agagcgtgga ggtcgatctg ccagacgcta
     1321 ctgaatcaca ggcggtgagc agtaacgata atccgccagt gattgttgaa gtgtctggta
     1381 ttggtcagta caccgtggtg gttgagaaag atcgcctgga gcgtttacca ccagagcagg
     1441 tggtggcgga agtgtccagc cgtttcaagg ccaacccgaa aacggtcttt ctgatcggtg
     1501 gcgcaaaaga tgtgccttac gatgaaataa ttaaagcact gaacttgtta catagtgcgg
     1561 gtgtgaaatc ggttggttta atgacgcagc ctatctaaac atctgcgttt cccttgcttg
     1621 aaagagagcg ggtaacaggc gaacagtttt tggaaaccga gagtgtcaaa ggcaaccgaa
     1681 caaaacgaca agctcaagcg ggcgataatt atttcagcag tgctgcatgt catcttattt
     1741 gcggcgctga tctggagttc gttcgatgag aatatagaag cttcagccgg aggcggcggt
     1801 ggttcgtcca tcgacgctgt catggttgat tcaggtgcgg tagttgagca gtacaaacgc
     1861 atgcaaagcc aggaatcaag cgcgaagcgt tctgatgagc agcgcaagat gaaagagcag
     1921 caggctgctg aagaactgcg tgagaaacaa gcggctgaac aggaacgcct gaagcaactt
     1981 gagaaagagc ggttagcggc tcaggagcag aaaaagcagg ctgaagaagc cgcaaaacag
     2041 gccgagttaa agcagaagca agcggaagag gcggcagcga aagcggcggc agatgctaaa
     2101 gcgaaggccg aagcggatgc taaagctgcg gaagaagcag cgaagaaagc ggcggcagac
     2161 gcgaagaaaa aagcagaagc agaagccgcc aaagccgcag tcgaagcgca gaaaaaagcc
     2221 gaggctgctg ctgcggcgct gaagaagaaa gctgaagcgg cagaagcagc tgcagctgaa
     2281 gcaagaaaga aagcggcaac cgaagctgct gaaaaagcca aggcagaagc tgagaagaaa
     2341 gcggcagcag agaaagcggc agccgacaaa aaagcagcag aaaaagcggc tgctgaaaag
     2401 gctgcagctg ataagaaagc ggcagcagag aaagcggcag ccgacaaaaa agcagcagaa
     2461 aaagcggctg ctgaaaaggc agcagctgat aagaaagcag cggcagaaaa agccgccgca
     2521 gacaaaaaag cggcagcggc aaaagcagca gctgaaaaag ccgctgcagc aaaagctgcc
     2581 gctgaggcag atgatatttt cggtgagcta agctctggta agaatgcacc gaaaacgggg
     2641 ggaggggcga aagggaacaa tgcttcgcct gccgggagtg gtaatactaa aaacaatggc
     2701 gcatcagggg ccgatatcaa taactatgcc gggcagatta aatctgctat cgaaagtaag
     2761 ttctatgacg catcgtccta tgcaggcaaa acctgtacgc tgcgcataaa actggcaccc
     2821 gatggtatgt tactggatat caaacctgaa ggtggcgatc ccgcactttg tcaagctgcg
     2881 ttggcagcag ctaaacttgc gaagatcccg aaaccaccaa gccaggcagt atatgaagtg
     2941 ttcaaaaacg cgccattgga cttcaaaccg taatcgcgat gttgactgtt ccgacggtca
     3001 acatcaggca ccggttgcca cggggttctg gtagttttgt gtattttagt ttgttaacat
     3061 tctgctaaat tatcgtgggc catcggtcca gataagggag atatgatgaa gcaggcatta
     3121 cgagtagcat ttggttttct catactgtgg gcatcagttc tgcatgctga agtccgcatt
     3181 gtgatcgaca gcggtgtaga ttccggtcgt cctattggtg ttgttccttt ccagtgggcg
     3241 gggcctggtg cggcacctga agatattggc ggcatcgttg ctgctgactt gcgtaacagc
     3301 ggtaaattta atccgttaga tcgtgctcgt ctgccacagc agccgggtag tgcgcaggaa
     3361 gtacaaccag ctgcatggtc cgcgctgggc attgacgctg tggttgtcgg tcaggtcact
     3421 ccgaatccgg atggttctta caatgttgct tatcaacttg ttgacactgg cggcgcaccg
     3481 ggtactgtac ttgctcagaa ctcgtacaaa gtgaacaagc agtggctgcg ttatgctggt
     3541 cataccgcca gtgacgaagt atttgaaaaa ctgaccggta ttaaaggtgc gttccgtacc
     3601 cgtattgcct acgttgttca gactaacggc ggtcagttcc cgtatgaact gcgcgtatct
     3661 gactatgacg gttacaacca gtttgtcgtt caccgttcac cgcaaccgct gatgtctccg
     3721 gcgtggtcac cagacggttc taaactggct tatgtgacct tcgaaagcgg tcgttccgcg
     3781 ctggttattc agacgctggc aaatggcgct gtacgtcagg tggcttcatt cccgcgtcac
     3841 aacggtgcac ctgcattctc gccagacggc agcaaactgg cattcgcctt gtcgaaaacc
     3901 ggtagcctga acctgtacgt aatggatttg gcttctggtc agatccgcca ggtaactgat
     3961 ggtcgcagta acaataccga accgacctgg ttcccggaca gccagaacct ggcatttact
     4021 tctgaccagg ccggtcgtcc gcaggtttat aaagtgaata tcaacggcgg tgcgccacaa
     4081 cgtattacct gggaaggttc gcagaaccag gatgcggatg tcagcagcga cggtaaattt
     4141 atggtaatgg tcagctccaa cggtgggcag cagcacattg ccaaacaaga tctggcaacg
     4201 ggaggcgtac aagttctgtc gtccacgttc ctggatgaaa cgccaagtct ggcacctaac
     4261 ggcactatgg taatctacag ctcttctcag gggatgggat ccgtgctgaa tttggtttct
     4321 acagatgggc gtttcaaagc gcgtcttccg gcaactgatg gacaggtcaa attccctgcc
     4381 tggtcgccgt atctgtgata ataattaatt gaatagtaaa ggaatcattg aaatgcaact
     4441 gaacaaagtg ctgaaagggc tgatgattgc tctgcctgtt atggcaattg cggcatgttc
     4501 ttccaacaag aacgccagca atgacggcag cgaaggcatg ctgggtgccg gcactggtat
     4561 ggatgcgaac ggcggcaacg gcaacatgtc ttccgaagag caggctcgtc tgcaaatgca
     4621 acagctgcag cagaacaaca tcgtttactt cgatctggac aagtacgata tccgttctga
     4681 cttcgctcaa atgctggatg cacacgcaaa cttcctgcgt agcaacccgt cttacaaagt
     4741 caccgtagaa ggtcacgcgg acgaacgtgg tactccggaa tacaacatct ccctgggtga
     4801 acgtcgtgcg aacgctgtta agatgtatct gcagggtaaa ggcgtttctg ctgaccagat
     4861 ctccatcgtt tcttacggta aagaaaaacc tgcagtactg ggtcatgacg aagcggcgta
     4921 tgccaaaaac cgtcgtgcgg tactggttta ctaagagaat tgcatgagca gtaacttcag
     4981 acatcaacta ttgagtctgt cgttactggt tggtatagcg gccccctggg ccgcttttgc
     5041 tcaggcacca atcagtagtg tcggctcagg ctcggtcgaa gaccgcgtca ctcaacttga
     5101 gcgtatttct aatgctcaca gccagctttt aacccaactc cagcaacaac tctctgataa
     5161 tcaatccgat attgattccc tgcgtggtca gattcaggaa aatcagtatc aactgaatca
     5221 ggtcgtggag cggcaaaagc agatcctgtt gcagatcgac agcctcagca gcggtggtgc
     5281 agcggcgcaa tcaaccagcg gcgatcaaag cggtgtggtg gcatcaacga cgccgacagc
     5341 tgatgctggt actgcgaatg ctggcgcgcc ggtgaaaagc ggtgatgcaa acacggatta
     5401 caatgcagct attgcgctgg tgcaggataa atcccgccag gatgacgcaa tggtggcatt
     5461 tcagaatttc atcaaaaatt accctgattc aacttacctg ccaaacgcca attattggct
     5521 gggtcagtta aactacaaca agggtaaaaa agatgatgcg gcgtattatt ttgcttcggt
     5581 agtgaaaaac tatccgaagt caccgaaggc tgcagatgcg atgtttaaag tcggcgtcat
     5641 catgcaggac aaaggtgaca ccgcaaaagc gaaagccgta taccagcagg ttatcagtaa
     5701 ataccctggt actgatggcg ctaaacaggc gcaaaaacgt ctgaacgcga tgtaatgcat
     5761 aacacacgac cagaagtcgc attatttctg gtcgtgtcgt gcgaatcata agcagttgag
     5821 tgatctacat cgaaattttt gttgcgctca agtctgaaat cagtaatata tgccgccgtt
     5881 gccacgggat atcaaacaaa ccgaaagcaa cgaaaaagtg ggtcgttagc tcagttggta
     5941 gagcagttga cttttaatca attggtcgca ggttcgaatc ctgcacgacc caccactaac
     6001 atagttagtt gtagtatcca gcgtagtatc gggtgattag ctcagctggg agagcacctc
     6061 ccttacaagg agggggtcgg cggttcgatc ccgtcatcac ccaccaccgg gtcgttagct
     6121 cagttggtag agcagttgac ttttaatcaa ttggtcgcag gttcgaatcc tgcacgaccc
     6181 accactgatt acggtgggtt cggtggaagt agtttgtagt atccagcgca gtatcgggtg
     6241 attagctcag ctgggagagc acctccctta caaggagggg gtcggcggtt cgatcccgtc
     6301 atcacccacc actcgggtcg ttagctcagt tggtagagca gttgactttt aatcaattgg
     6361 tcgcaggttc gaatcctgca cgacccacca attttaaaca ttggttccga gtagaaatct
     6421 ttcaggtaac acccgtatgg gtcgttagct cagttggtag agcagttgac ttttaatcaa
     6481 ttg
//
LOCUS       DAHHTG010000190         6388 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.193, whole genome shotgun sequence.
ACCESSION   DAHHTG010000190 DAHHTG010000000
VERSION     DAHHTG010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6388)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6388)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6388
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.193"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            1..506
                     /locus_tag="NBI74_004234"
                     /pseudo
     CDS             1..506
                     /locus_tag="NBI74_004234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013124600.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            515..1375
                     /locus_tag="NBI74_004235"
     CDS             515..1375
                     /locus_tag="NBI74_004235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HCD1934471.1"
                     /translation="MFTAIAPAIWGSTYIVTTQYLPNFSPMTVAMLRALPAGLLLVMI
                     VRQIPTGIWWMRIFILGALNISLFWSLLFISVYRLPGGVAATVGAVQPLMVVFISAAL
                     LGSPIRLMAVLGAICGTAGVALLVLTPNAALDPVGVAAGLAGAVSMAFGTVLTRKWQP
                     PVPLLTFTAWQLAAGGLLLVPVALVFDPPIPMPTGTNVLGLAWLGLIGAGLTYFLWFR
                     GISRLEPTVVSLLGFLSPGTAVLLGWLFLDQTLSALQIIGVLLVIGSIWLGQRSNRTP
                     RARIACRKSP"
     gene            complement(1407..2606)
                     /gene="tet(A)"
                     /locus_tag="NBI74_004236"
     CDS             complement(1407..2606)
                     /gene="tet(A)"
                     /locus_tag="NBI74_004236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(A)"
                     /protein_id="HCD1934472.1"
                     /translation="MKPNRPLIVILSTVALDAVGIGLIMPVLPGLLRDLVHSNDVTAH
                     YGILLALYALMQFACAPVLGALSDRFGRRPVLLVSLAGAAVDYAIMATAPFLWVLYIG
                     RIVAGITGATGAVAGAYIADITDGDERARHFGFMSACFGFGMVAGPVLGGLMGGFSPH
                     APFFAAAALNGLNFLTGCFLLPESHKGERRPLRREALNPLASFRWARGMTVVAALMAV
                     FFIMQLVGQVPAALWVIFGEDRFHWDATTIGISLAAFGILHSLAQAMITGPVAARLGE
                     RRALMLGMIADGTGYILLAFATRGWMAFPIMVLLASGGIGMPALQAMLSRQVDEERQG
                     QLQGSLAALTSLTSIVGPLLFTAIYAASITTWNGWAWIAGAALYLLCLPALRRGLWSG
                     AGQRADR"
     gene            2712..3338
                     /gene="tetR(A)"
                     /locus_tag="NBI74_004237"
     CDS             2712..3338
                     /gene="tetR(A)"
                     /locus_tag="NBI74_004237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000164043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance transcriptional
                     repressor TetR(A)"
                     /protein_id="HCD1934473.1"
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWH
                     FRNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAG
                     TRPGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGGTVEQAPLS
                     PLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
     gene            complement(3370..3606)
                     /locus_tag="NBI74_004238"
     CDS             complement(3370..3606)
                     /locus_tag="NBI74_004238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000844627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase"
                     /protein_id="HCD1934474.1"
                     /translation="MAGQSLSVGFRPFPQTTPTRATAHERTPQQERQKAAREAERGRE
                     AWTLGQGMKKPVAGCYGRLTRWKGGGDVVYMALL"
     gene            complement(3660..4496)
                     /locus_tag="NBI74_004239"
     CDS             complement(3660..4496)
                     /locus_tag="NBI74_004239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000480972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(6)-Id"
                     /protein_id="HCD1934475.1"
                     /translation="MFMPPVFPAHWHVSQPVLIADTFSSLVWKVSLPDGTPAIVKGLK
                     PIKDIADELRGADYLVWRNGRGAVRLLGRENNLMLLEYAGERMLSHIVAEHGDYQATE
                     IAAELMAKLYAASEEPLPSALLPIRDRFAALFQRARDDQNAGCQTDYVHAAIIADQMM
                     SNASELRGLHGDLHHENIMFSSRGWLVIDPVGLVGEVGFGAANMFYDPADRDDLCLDP
                     RRIAQMADAFSRALDVDPRRLLDQAYAYGCLSAAWNADGEEEQRDLAIAAAIKQVRQT
                     SY"
     gene            complement(4496..5299)
                     /locus_tag="NBI74_004240"
                     /note="aph(3'')-Ib"
     CDS             complement(4496..5299)
                     /locus_tag="NBI74_004240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010890156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(3'')-Ib"
                     /protein_id="HCD1934476.1"
                     /translation="MNRTNIFFGESHSDWLPVRGGESGDFVFRRGDGHAFAKIAPASR
                     RGELAGERDRLIWLKGRGVACPEVINWQEEQEGACLVITAIPGVPAADLSGADLLKAW
                     PSMGQQLGAVHSLSVDQCPFERRLSRMFGRAVDVVSRNAVNPDFLPDEDKSTPQLDLL
                     ARVERELPVRLDQERTDMVVCHGDPCMPNFMVDPKTLQCTGLIDLGRLGTADRYADLA
                     LMIANAEENWAAPDEAERAFAVLFNVLGIEAPDRERLAFYLRLDPLTWG"
     gene            complement(5360..6175)
                     /gene="sul2"
                     /locus_tag="NBI74_004241"
     CDS             complement(5360..6175)
                     /gene="sul2"
                     /locus_tag="NBI74_004241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonamide-resistant dihydropteroate synthase
                     Sul2"
                     /protein_id="HCD1934477.1"
                     /translation="MNKSLIIFGIVNITSDSFSDGGRYLAPDAAIAQARKLMAEGADV
                     IDLGPASSNPDAAPVSSDTEIARIAPVLDALKADGIPVSLDSYQPATQAYALSRGVAY
                     LNDIRGFPDAAFYPQLAKSSAKLVVMHSVQDGQADRREAPAGDIMDHIAAFFDARIAA
                     LTGAGIKRNRLVLDPGMGFFLGAAPETSLSVLARFDELRLRFDLPVLLSVSRKSFLRA
                     LTGRGPGDVGAATLAAELAAAAGGADFIRTHEPRPLRDGLAVLAALKETARIR"
BASE COUNT         1340 a         1910 c         1913 g         1225 t
ORIGIN      
        1 acgttctgat ccggcgtgcc cggcaaggca gcggcctcgt gacggatctg gtgcccggcc
       61 agtccggtgg aggtaaaggt aaggggcgct tgccggaacc ggtcgagcgc gtcatccacg
      121 agctactgca aaagcggttc ctgaccaagc agaagcgcag cctagcggcc tttcaccgcg
      181 aagtcactca ggtgtgcaag gctcaaaaac tgcgagtgcc ggcgcgcaat accgtggcct
      241 tacggatcgc tagccttgac ccgcgcaagg tcatccgccg gcgggaaggc caggatgccg
      301 ctcgtgacct acaaggtgtg ggcggcgagc ctcctgccgt gaccgcgccg ctggagcagg
      361 tgcagataga ccatacggtc atcgacctga tcgtggtcga tgaccgcgac cggcaaccta
      421 ttggccgccc gtacctgacc ctcgccatcg acgtgttcac ccgctgcgtg ctcggcatgg
      481 tcgtcacgct ggaagcgccg tctgccccga tttattgttc acagcgatag cacctgccat
      541 ttggggcagc acctacattg tcaccaccca atacctgccg aacttctcac cgatgacggt
      601 cgcgatgctg cgggcgttgc cggcgggttt attgctcgtg atgatcgtcc gacagattcc
      661 aacgggaatc tggtggatgc gcatcttcat cctcggcgca cttaatattt cgctattctg
      721 gagcttgttg tttatttcgg tctaccgcct gccgggcggg gtcgcggcga cggtaggcgc
      781 tgtgcagccg ctgatggtcg tgttcatctc tgccgctctg ctaggtagcc cgatacgatt
      841 gatggcggtc ctgggggcta tttgcggaac tgcgggcgtg gcgctgttgg tgttgacacc
      901 aaacgcagcg ctagatcctg tcggcgtcgc agcgggcctg gcgggggcgg tttccatggc
      961 gttcggaacc gtgctgaccc gcaagtggca acctcccgtg cctctgctca cctttaccgc
     1021 ctggcaactg gcggccggag gacttctgct cgttccagta gctttagtgt ttgatccgcc
     1081 aatcccgatg cctacaggaa ccaatgttct cggcctggcg tggctcggcc tgatcggagc
     1141 gggtttaacc tacttccttt ggttccgggg gatctcgcga ctcgaaccta cagttgtttc
     1201 cttactgggc tttctcagcc cggggaccgc cgtgttgcta ggatggttgt tcttggatca
     1261 gacgctgagt gcgcttcaaa tcatcggcgt cctgctcgtg atcgggagta tctggctggg
     1321 ccaacgttcc aaccgcactc ctagggcgcg tatagcttgc cggaagtcgc cttgacccgc
     1381 atggcatagg cctatcgttt ccacgatcag cgatcggctc gttgccctgc gccgctccaa
     1441 agcccgcgac gcagcgccgg caggcagagc aagtagaggg cagcgcctgc aatccatgcc
     1501 cacccgttcc acgttgttat agaagccgca tagatcgccg tgaagaggag gggtccgacg
     1561 atcgaggtca ggctggtgag cgccgccagt gagccttgca gctgcccctg acgttcctca
     1621 tccacctgcc tggacaacat tgcttgcagc gccggcattc cgatgccacc cgaagcaagc
     1681 aggaccatga tcgggaacgc catccatccc cgtgtcgcga aggcaagcag gatgtagcct
     1741 gtgccgtcgg caatcattcc gagcatgagt gcccgccttt cgccgagccg ggcggctaca
     1801 gggccggtga tcattgcctg ggcgagtgaa tgcagaatgc caaatgcggc aagcgaaatg
     1861 ccgatcgtgg tcgcgtccca gtgaaagcga tcctcgccga aaatgaccca aagcgcggcc
     1921 ggcacctgtc cgacaagttg catgatgaag aagaccgcca tcagggcggc gacgacggtc
     1981 atgccccggg cccaccggaa cgaagcgagc gggttgagag cctcccggcg taacggccgg
     2041 cgttcgcctt tgtgcgactc cggcaaaagg aaacagcccg tcaggaaatt gaggccgttc
     2101 aaggctgccg cggcgaagaa cggagcgtgg ggggagaaac cgcccatcag cccaccgagc
     2161 acaggtcccg cgaccatccc gaacccgaaa caggcgctca tgaagccgaa gtgccgcgcg
     2221 cgctcatcgc catcagtgat atcggcaata taagcgccgg ctaccgcccc agtcgccccg
     2281 gtgatgccgg ccacgatccg cccgatatag agaacccaaa ggaaaggcgc cgtcgccatg
     2341 atggcgtagt cgacagcagc gccggccagc gagacgagca agaccggccg ccgcccgaaa
     2401 cgatccgaca gcgcgcccag cacaggtgcg caggcaaatt gcatcaacgc atacagcgcc
     2461 agcagaatgc catagtgggc ggtgacgtcg ttcgagtgaa ccagatcgcg caggaggccc
     2521 ggcagcaccg gcataatcag gccgatgccg acagcgtcga gcgcgacagt gctcagaatt
     2581 acgatcaggg gtctgttggg tttcacgtct ggcctccgga ccagcctccg ctggtccgat
     2641 tgaacgcgcg gattctttat cactgataag ttggtggaca tattatgttt atcagtgata
     2701 aagtgtcaag catgacaaag ttgcagccga atacagtgat ccgtgccgcc ctggacctgt
     2761 tgaacgaggt cggcgtagac ggtctgacga cacgcaaact ggcggaacgg ttgggggttc
     2821 agcagccggc gctttactgg cacttcagga acaagcgggc gctgctcgac gcactggccg
     2881 aagccatgct ggcggagaat catacgcatt cggtgccgag agccgacgac gactggcgct
     2941 catttctgat cgggaatgcc cgcagcttca ggcaggcgct gctcgcctac cgcgatggcg
     3001 cgcgcatcca tgccggcacg cgaccgggcg caccgcagat ggaaacggcc gacgcgcagc
     3061 ttcgcttcct ctgcgaggcg ggtttttcgg ccggggacgc cgtcaatgcg ctgatgacaa
     3121 tcagctactt cactgttggg gccgtgcttg aggagcaggc cggcggcacc gttgaacagg
     3181 ctccgctctc gccgctgttg cgggccgcga tagacgcctt cgacgaagcc ggtccggacg
     3241 cagcgttcga gcagggactc gcggtgattg tcgatggatt ggcgaaaagg aggctcgttg
     3301 tcaggaacgt tgaaggaccg agaaagggtg acgattgatc aggaccgctg ccggagcgca
     3361 acccactcac tacagcagag ccatgtagac aacatcccct ccccctttcc accgcgtcag
     3421 acgcccgtag cagcccgcta cgggcttttt catgccctgc cctagcgtcc aagcctcacg
     3481 gccgcgctcg gcctctctgg cggccttctg gcgctcctgc tgcggcgtcc gctcgtgggc
     3541 cgtggcgcgg gtcggggtcg tttgcgggaa ggggcggaat cctacgctaa ggctttggcc
     3601 agcgatattc tccggtgaga ttgatgtgtt cccaggggat aggagaagtc gcttgatatc
     3661 tagtatgacg tctgtcgcac ctgcttgatc gcggccgcga tagctagatc gcgttgctcc
     3721 tcttctccat ccgcgttcca agctgcggaa aggcacccat aagcgtacgc ctggtcgagc
     3781 aggcgacgcg gatcgacgtc cagcgcacga gagaatgcgt ccgccatctg tgcaatgcgt
     3841 ctaggatcga gacaaaggtc gtctctgtca gccggatcgt agaacatatt ggcggcgcca
     3901 aagcccactt caccgaccag accgacggga tctatcacca gccagccgcg actggagaac
     3961 atgatgtttt catgatgcag atcgccatgt agcccacgca gttccgaggc attgctcatc
     4021 atttgatcgg ctataatcgc cgcgtggacg tagtcagttt gacaacctgc gttttgatca
     4081 tcgcgcgccc gctgaaacaa agctgcaaag cgatcccgga tcgggagaag ggcagaaggc
     4141 aggggttcct cagatgcggc atacagcttc gccattagtt ccgctgcaat ttcggtcgcc
     4201 tggtagtcgc cgtgctcggc aacgatgtga gagagcattc gctccccggc atattcgagc
     4261 aacatcagat tgttctcacg accgagcaac cggactgctc ccctcccatt gcgccatacc
     4321 agatagtcgg ccccgcgcag ttcatcagca atgtctttta taggtttcaa tcccttgacg
     4381 attgcaggag tcccgtctgg caatgaaact ttccaaacga ggctggaaaa ggtgtccgca
     4441 atgagaacag gttgcgaaac gtgccaatga gcaggaaaaa caggcggcat gaacatcaac
     4501 cccaagtcag agggtccaat cgcagataga aggcaaggcg ttcgcggtcg ggggcttcga
     4561 tccccaatac attgaatagg acagcgaagg cgcgctctgc ttcatctggc gctgcccagt
     4621 tctcttcggc gttagcaatc atgagtgcca aatcggcata gcgatctgct gttccgagcc
     4681 gcccaaggtc gatcagaccc gtgcattgaa gagttttagg gtccaccatg aagttcggca
     4741 tgcagggatc accatggcaa acaaccatat cggtgcgctc ttggtcgagc cgcaccggta
     4801 gctctcgttc gacacgagcc aaaagatcga gctgcggcgt actcttgtcc tcgtccggta
     4861 agaagtcggg attgacggca ttgcgggaca ccacatcaac ggcgcgtccg aacattcgcg
     4921 acagcctgcg ctcaaacgga cattgatcaa ccgataggct gtgaacagcg ccaagttgct
     4981 gccccattga cggccacgct ttgagcaaat ccgctccaga cagatcagcc gccggtactc
     5041 ccggaattgc cgttatcacc aagcatgcac cctcctgttc ctcctgccag ttgatgacct
     5101 cggggcaagc cacacctcga cctttgagcc aaatgaggcg gtcacgctct ccagcgagct
     5161 caccgcggcg ggaagcaggt gcgattttcg cgaaggcatg cccgtcacca cgtcgaaaaa
     5221 caaaatcacc agattctccg cctctgacag gcaaccagtc agaatgcgat tcaccaaaaa
     5281 aaatattagt tcgattcaat ggaggttcct tcagttttct gatgaagcgc gaatatagag
     5341 aaatatcccg aatgtgcagt taacgaattc ttgcggtttc tttcagcgcc gccaataccg
     5401 ccagcccgtc gcgcaagggg cgcggctcgt gtgtgcggat gaagtcagct ccacctgcgg
     5461 cggcggcaag ctctgcagcg agtgtcgcgg ccccgacatc ccccggacca cggcctgtga
     5521 gcgcgcgcag aaaggatttg cgcgaaacag acagaagcac cggcaaatcg aagcgcagcc
     5581 gcaattcatc gaaccgcgcc agcaccgaga gcgaggtttc gggagcagcc cccagaaaaa
     5641 accccatgcc gggatcaagg acaaggcggt tgcgtttgat accggcaccc gtcagcgccg
     5701 cgatgcgcgc gtcaaagaac gccgcaatgt gatccatgat gtcgccagcg ggtgcctcgc
     5761 gccgatctgc ctgcccgtct tgcaccgaat gcataacgac gagtttggca gatgatttcg
     5821 ccaattgcgg atagaacgca gcgtctggaa aaccgcgaat atcattgaga taggccacac
     5881 cacgcgacaa ggcataggct tgcgtcgcgg gttgataact gtcgagcgag acgggaatgc
     5941 catctgcctt gagcgcgtcc agcaccggcg cgatacgcgc gatttctgtg tcggacgaaa
     6001 caggcgcggc gtcgggattg ctggatgccg gaccgaggtc gatcacatct gccccctcgg
     6061 ccatcagctt acgcgcctgc gcaatggctg cgtctggcgc cagataccgg cctccatcgg
     6121 agaaactgtc cgaggttatg ttgacgatgc cgaaaatgat gagcgattta ttcatggggg
     6181 cttctataat aataataatc gagcatgagt atcatacgga tgctcgggtc gaaagggaat
     6241 ccccaggcga gtaacctgtt tgcggtgatc cattagctgc aggagcagaa gagcatacat
     6301 ctggaagcaa agccaggaaa gcggcctatg gagctgtgcg gcagcgctca gtaggcaatt
     6361 tttcaaaata ttgttaagcc ttttctga
//
LOCUS       DAHHTG010000191         6356 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.194, whole genome shotgun sequence.
ACCESSION   DAHHTG010000191 DAHHTG010000000
VERSION     DAHHTG010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6356)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6356)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6356
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.194"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            complement(13..960)
                     /gene="fmt"
                     /locus_tag="NBI74_004242"
     CDS             complement(13..960)
                     /gene="fmt"
                     /locus_tag="NBI74_004242"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HCD1934478.1"
                     /translation="MSESLRIIFAGTPDFAARHLDALLSSGHNIVGVFTQPDRPAGRG
                     KKLMPSPVKVLAEDKGLPVFQPVSLRPQENQQLVADLQADVMVVVAYGLILPKAVLEM
                     PRLGCINVHGSLLPRWRGAAPIQRSLWAGDAETGVTIMQMDVGLDTGDMLYKLSCPIT
                     AEDTSGTLYDKLAELGPQGLITTLKQLADGTAKPEVQDETLVTYAEKLSKEEARIDWS
                     LSAAQLERCIRAFNPWPMSWLEIEGQPVKVWKASVIDTTTKAAPGTILEANKQGIQVA
                     TGDGILNLLSMQPAGKKAMSVQDLLNSRREWFVPGNRLA"
     gene            complement(975..1484)
                     /gene="def"
                     /locus_tag="NBI74_004243"
     CDS             complement(975..1484)
                     /gene="def"
                     /locus_tag="NBI74_004243"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HCD1934479.1"
                     /translation="MSVLQVLHIPDERLRKVAKPVEEVNAEIQRIVDDMFETMYAEEG
                     IGLAATQVDIHQRIIVIDVSENRDERLVLINPELLEKSGETGIEEGCLSIPEQRALVP
                     RAEKVKIRALDRDGKPFELEADGLLAICIQHEMDHLVGKLFMDYLSPLKQQRIRQKVE
                     KLDRLKARA"
     gene            1614..2738
                     /gene="dprA"
                     /locus_tag="NBI74_004244"
     CDS             1614..2738
                     /gene="dprA"
                     /locus_tag="NBI74_004244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HCD1934480.1"
                     /translation="MVDTEIWLRLMSISSLYGDDMVRIAHWLAKQSHIDAVVLQQTGL
                     TLRQAQRFLSFPRKSIESSLCWLEQPNHHLIPADSEFYPPQLLATTDYPGALFVEGEL
                     HALHSFQLAVVGSRAHSWYGERWGRLFCETLATRGVTITSGLARGIDGVAHKAALQVN
                     GVSIAVLGNGLNTIHPRRHAPLAASLLEQGGALVSEFPLDVPPLAYNFPRRNRIISGL
                     SKGVLVVEAALRSGSLVTARCALEQGREVFALPGPIGNPGSEGPHWLIKQGAILVTEP
                     EEILENLQFGLHWLPDAPENSFYSPDQEDVALPFPELLANVGDEVTPVDVVAERAGQP
                     VPEVVTQLLELELAGWIAAVPGGYVRLRRACHVRRTNVFV"
     gene            2710..3183
                     /locus_tag="NBI74_004245"
     CDS             2710..3183
                     /locus_tag="NBI74_004245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF494 domain-containing protein"
                     /protein_id="HCD1934481.1"
                     /translation="MFDVLMYLFETYIHTEAELRVDQDKLEQDLTDAGFDREDIYNAL
                     LWLEKLADYQEGLAEPMQLASDPLSMRIYTPEECERLDASCRGFLLFLEQIQVLSLET
                     REMVIERVLALDTAEFDLEDLKWVILMVLFNIPGCENAYQQMEELLFEVNEGMLH"
     gene            3212..3754
                     /locus_tag="NBI74_004246"
     CDS             3212..3754
                     /locus_tag="NBI74_004246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase DNA-binding C4 zinc finger
                     domain-containing protein"
                     /protein_id="HCD1934482.1"
                     /translation="MAKSALFTVRNNESCPKCGAELVIRSGKHGPFLGCSQYPACGYV
                     RPLKSSADGHIVKVLEGQVCPACGANLVLRQGRFGMFIGCSNYPECEHTELIDKPDET
                     AITCPQCRTGHLVQRRSRYGKTFHSCDRYPECQFAINFKPIAGECPECHYPLLIEKKT
                     AQGVKHFCASKQCGKPISAE"
     gene            3759..4331
                     /gene="tsaC"
                     /locus_tag="NBI74_004247"
     CDS             3759..4331
                     /gene="tsaC"
                     /locus_tag="NBI74_004247"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase type 1
                     TsaC"
                     /protein_id="HCD1934483.1"
                     /translation="MNNNLQGDAIAAAIDVLNEERVIAYPTEAVFGVGCDPDSETAVM
                     RLLELKRRPVDKGLILIAANYEQLKPYIDDTMLTDAQRETIFSRWPGPVTFVFPAPAT
                     TPRWLTGRFDSLAVRVTDHPLVVALCQAYGKPLVSTSANLSGLPPCRTVDEVRAQFGA
                     AFPVVPGETGGRLNPSEIRDALTGELFRQG"
     gene            4336..5154
                     /gene="aroE"
                     /locus_tag="NBI74_004248"
     CDS             4336..5154
                     /gene="aroE"
                     /locus_tag="NBI74_004248"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HCD1934484.1"
                     /translation="METYAVFGNPIAHSKSPFIHQQFAQQLNIEHPYGRVLTPINDFI
                     NTLNTFFSAGGKGANVTVPFKEEAFARADELTERAALAGAVNTLKRLEDGRLLGDNTD
                     GIGLLSDLERLSFIRPGLRILLIGAGGASRGVLLPLLSLDCAVTITNRTVSRAEELAK
                     LFAHTGSIHALGMHELEGHEFDLIINATSSGISGDIPAIPSSLIHPGIYCYDMFYQKG
                     KTPFLAWCEQRGSKRTADGLGMLVAQAAHAFLLWHGVLPDVEPVIKLLQQELSA"
     gene            5151..5408
                     /locus_tag="NBI74_004249"
     CDS             5151..5408
                     /locus_tag="NBI74_004249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1488 domain-containing protein"
                     /protein_id="HCD1934485.1"
                     /translation="MNQAIQFPDREEWDENKKCVCFPALVNGMQLTCAISGESLAYRF
                     TGDTPEQWLESFRQHRWDLEEEAENLIQEQSEDDQGWVWLP"
     gene            complement(5384..5938)
                     /locus_tag="NBI74_004250"
     CDS             complement(5384..5938)
                     /locus_tag="NBI74_004250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HCD1934486.1"
                     /translation="MSEVLRPYRDLFPQIGQRVMIDDSSVVIGDVRLADDVGIWPLVV
                     IRGDVHYVQIGARTNIQDGSMLHVTHKSSYNPAGNPLTIGEDVTIGHKVMLHGCTIGN
                     RVLVGMGSILLDGAIVEDDVMIGAGSLVPQNKRLESGYLYLGSPVKQIRPLSDEEKAG
                     LRYSANNYVKWKDEYMDQGNHTQP"
BASE COUNT         1600 a         1501 c         1596 g         1659 t
ORIGIN      
        1 aagagtggac tatcaggcca gacggttgcc cggtacaaac cattcccgac gagaattcag
       61 gaggtcttgc acgctcatcg ctttcttacc tgcaggttgc attgagagca gattcaggat
      121 gccatcacct gtcgctacct gaatgccttg tttattggct tcaaggattg ttcctggcgc
      181 agcctttgtt gtcgtatcga tgacagatgc tttccagact ttaaccggct gcccttcaat
      241 ttccagccag ctcattggcc atggattgaa agcgcgaatg caacgttcaa gctgtgctgc
      301 cgaaagtgac cagtcaatac gcgcttcttc tttactcaac ttttcggcgt aagtgacaag
      361 agtttcgtcc tgaacttctg gtttcgccgt gccgtctgcc agttgtttca acgtggtgat
      421 aagcccttgt gggccaagct ctgccagctt gtcgtacagc gtaccactgg tatcttctgc
      481 agtaatcggg caggagagct tatagagcat gtcaccggtg tctaaaccaa catccatttg
      541 cataatggtc acaccagttt ctgcatcacc cgcccatagt gagcgttgga ttggtgctgc
      601 accgcgccag cgtggcagca gtgaaccatg aacgttgata cagccaagac gcggcatctc
      661 cagcacagct ttaggcagaa ttaaaccata ggcgacgacg accataacat cagcctgcag
      721 atcggcgacc agttgctggt tttcttgtgg acgcagggaa acaggttgaa aaacgggcag
      781 acctttgtcc tcagccagaa ctttaaccgg gctgggcatc agttttttac cgcgtcctgc
      841 cggtcggtct ggctgggtga acacgccaac gatgttatga ccagaagaca acagcgcgtc
      901 gagatgacgc gctgcaaagt caggtgtacc cgcaaaaata atacgtagtg attctgacac
      961 gttaattctt atccttaagc ccgggctttc agacgatcca gtttttcaac tttctgacga
     1021 atacgttgtt gtttcagcgg tgacagataa tccataaaca gtttgccgac caggtgatcc
     1081 atctcatgct gaatacagat ggctaacaga ccgtctgctt ccagttcaaa tggtttaccg
     1141 tcgcggtcca gggcgcgaat tttaactttc tctgcgcgtg gcactaaagc acgttgttca
     1201 gggatcgaca ggcaaccttc ttcaatgcct gtttcgccgc ttttttctaa aagctccgga
     1261 ttgattaaca ccagccgttc gtcacggttt tccgaaacat caattacaat gatacgttga
     1321 tggatatcaa cctgggttgc cgccaggcca ataccttctt ctgcgtacat cgtctcgaac
     1381 atatcatcga cgatacgctg aatttctgca ttcacttctt ctaccggttt agcaactttg
     1441 cgaagccgct cgtccggaat atgtaacact tgcaaaactg acataaatct ccagagatgt
     1501 gttcaggagt tagaaagatt atttcttcta ttctagacaa atccccctct gattgacagc
     1561 atcactgacc aatcgcaaag attgctaagg ctgcttatgg cagggagata aggatggtcg
     1621 atacagaaat ttggctgcgt ttaatgagca tcagcagctt gtatggcgat gatatggtcc
     1681 gtatagctca ctggctggca aaacagtcgc atattgatgc ggttgtattg cagcaaacag
     1741 ggcttacatt gcggcaggca caacgctttc tttcatttcc gcgaaagagt atcgaaagct
     1801 cactttgttg gttggagcaa cccaaccatc atttaatccc tgcggacagc gaattttatc
     1861 ctcctcaact tctggcgacg acagattacc ccggcgcact gtttgttgaa ggagaactgc
     1921 acgcgttgca ttcatttcag cttgccgtag tggggagtcg ggcgcattca tggtatggcg
     1981 agcgatgggg acgattattt tgcgaaactc tggcgacgcg tggagtgaca attacgagtg
     2041 gactggcgcg tggaatcgat ggtgtggcgc ataaagcggc cttacaggta aatggcgtta
     2101 gcattgctgt attggggaat ggacttaata ccattcatcc ccgccgccat gctccactgg
     2161 ctgccagtct acttgagcaa ggtggtgctc tcgtctcgga atttcccctc gatgttccac
     2221 cccttgctta caatttccca cgaagaaatc gcattatcag tggtctaagt aagggtgtac
     2281 tggtggtgga agcggctttg cgcagtggtt cgctggtgac agcacgttgt gcgcttgagc
     2341 aggggcgtga agtttttgct ttgccaggtc caatagggaa tccgggaagc gaagggcctc
     2401 actggttaat aaaacaaggt gcgattcttg tgacggaacc ggaagaaatt ctggaaaact
     2461 tgcaatttgg attgcactgg ttgccagacg cccctgaaaa ttcattttat tcaccagatc
     2521 aggaagacgt ggcattgcca tttcctgagc tcctggctaa cgtaggagat gaggtaacac
     2581 ctgttgacgt cgtcgctgaa cgtgccggcc aacctgtgcc agaggtagtt actcaactac
     2641 tcgaactgga gttagcagga tggatcgcag ctgtacccgg cggctatgtc cgattgagga
     2701 gggcatgcca tgttcgacgt actaatgtat ttgtttgaaa cctatattca cacagaagct
     2761 gagttgcgtg tggatcaaga caaacttgaa caggatctta ccgacgcagg atttgatcga
     2821 gaagatatct acaatgccct gctttggctg gaaaagcttg cggattatca ggaaggactg
     2881 gcagaaccga tgcaactggc ctctgatcca ctctccatgc gtatttatac ccctgaagag
     2941 tgtgaaagac tggatgccag ctgtcgtggt tttttgcttt tccttgagca aattcaggtg
     3001 cttagccttg aaacccgtga aatggtgata gagcgagtgc tcgcgctgga taccgcagag
     3061 ttcgacctgg aagacctgaa atgggtaatc ctgatggtgt tgttcaatat tccgggttgc
     3121 gaaaatgcgt accagcaaat ggaagaatta ctctttgaag tgaatgaagg tatgctgcat
     3181 taatcatttc tttaattcag cataagttgt tatggcgaaa tcagcactgt tcacggtgcg
     3241 taataatgag tcctgcccaa agtgcggggc tgaactagtt attcgatccg ggaaacacgg
     3301 tccgtttctt ggatgctcac agtatccggc atgtggctac gtccgtcctc tgaaatcttc
     3361 agcggatgga catattgtca aagttctgga ggggcaggtt tgccctgcat gtggcgcaaa
     3421 tctggtatta cgccagggac gctttggtat gtttattggt tgcagtaact accctgaatg
     3481 cgaacatacc gaacttatcg ataaaccgga cgaaacagca attacatgcc cccaatgccg
     3541 gacgggccat ctggttcagc gccgctcccg ttatggcaaa acatttcact cctgtgatcg
     3601 ctacccggag tgtcaatttg ccattaactt caaacccata gctggagaat gccctgagtg
     3661 ccattatccg ctactcatcg aaaagaaaac cgcgcagggt gtaaaacact tttgtgccag
     3721 taaacaatgt ggaaagccga tttcggcgga ataataacgt gaataataac ctgcaaggag
     3781 acgctatcgc agctgcgata gatgtcctca atgaagaacg tgtcatcgcc tatccaacgg
     3841 aagccgtttt cggtgtcggg tgcgatcctg atagcgaaac agcagtgatg agactgttgg
     3901 agttaaaacg gcgtccggtt gataaggggc tgattttaat cgcggcaaat tacgagcagc
     3961 ttaaacccta tattgatgac accatgctga ctgacgcgca gcgtgaaacc attttttccc
     4021 gctggccagg tcctgtaacc tttgtctttc ccgcgcctgc gacaacaccg cgctggttga
     4081 cgggccgctt tgattcgctt gctgtacgag tcaccgacca tccgttggtg gttgctttgt
     4141 gccaggctta tggtaaaccg ctggtttcta ccagtgccaa cttgagtgga ttgccacctt
     4201 gtcgaacagt agacgaagtt cgcgcacaat ttggcgctgc gttcccggtt gtgcctggtg
     4261 aaacgggggg gcgtttaaat ccttcagaaa tccgcgatgc cctgacgggt gaactgtttc
     4321 gacaggggta acataatgga aacctatgct gtttttggta atccgatagc ccacagcaaa
     4381 tcgccattca ttcatcagca atttgctcag caactgaata ttgaacatcc ctatgggcgt
     4441 gtgttgacac ccatcaatga tttcatcaac acactgaaca ctttctttag tgctggtggt
     4501 aaaggtgcga atgtgacggt gccttttaaa gaagaggctt ttgccagagc ggatgagctt
     4561 actgaacggg cagcgttggc tggtgctgtt aataccctca agcggttaga agatggacgc
     4621 ttgctgggtg acaataccga tggtataggt ttgttaagcg atctggaacg tctgtctttt
     4681 atccgtcctg gtttacgtat tttgctcatc ggcgctggtg gagcatctcg cggcgtacta
     4741 ctgccactcc tttccctgga ctgtgcggtg acaatcacta atcggacggt atcccgcgcg
     4801 gaagagttgg ctaaattgtt tgcgcacact ggcagtattc acgcgttggg tatgcacgaa
     4861 ctggaaggtc atgagtttga tctcattatt aatgcaacat ccagtggcat cagtggtgat
     4921 attccggcga tcccgtcatc gctcattcat ccaggcattt attgctatga catgttctat
     4981 cagaaaggaa aaactccttt tctggcatgg tgtgagcagc gaggctcaaa gcgtactgct
     5041 gatggtttag gaatgctggt ggcacaagcg gctcatgcct tccttctttg gcatggcgtt
     5101 ctgcctgatg tagaaccggt catcaaacta ttgcaacagg agttgtcagc gtgaatcagg
     5161 ccatccagtt tccggatagg gaagagtggg acgagaataa aaaatgtgta tgttttcccg
     5221 ctctcgtgaa tggtatgcaa ctgacatgtg cgatctctgg cgagagtctg gcgtatcgct
     5281 ttactggaga tacaccagaa cagtggttag agagttttcg tcagcatcgc tgggacctgg
     5341 aagaagaagc ggaaaactta attcaggaac aaagtgaaga tgatcaaggt tgggtgtggt
     5401 taccctgatc catatattcg tccttccatt tcacgtagtt atttgcggaa tagcgtaacc
     5461 cagccttctc ttcatcactt aacgggcgga tctgtttgac ggggctaccg agatacagat
     5521 atccgctctc cagccgttta ttttgtggga ccaggctacc tgcaccaatc atcacatcat
     5581 cttctactat tgcgccatca agtaaaattg agcccatccc aaccaaaact cgattgccaa
     5641 tggtgcagcc gtggagcatc accttgtgac caatagtgac atcttcgcca atggttaatg
     5701 ggttgccagc tgggttgtac gaggatttat gagtgacgtg caacatactg ccatcctgga
     5761 tattggtgcg tgctccgatc tgtacataat gtacatctcc acgaatcaca acgagcggcc
     5821 agatccccac atcatcagcc agacgaacgt caccaatcac gacgctgcta tcgtcgatca
     5881 ttacgcgctg accgatttgt ggaaaaagat cgcggtatgg gcgtaaaacc tcagacatac
     5941 tcacctcaac aataaatgat ttactaatga ctttgggtac attattggcc ttgtgcaagt
     6001 cttttagtat gcaaaaaagc accgttttgt gtgcgaatgc agcaaaaagg gtgaaaaaac
     6061 aacaaacaga aaaaaagatc aaaaaagtac ttgtgcaaaa aattgggatc cctataatgc
     6121 gcctccgttg agacgacaac gtgaaacact tcacaagata gtcgtaacaa caaagagaaa
     6181 aatcctgaaa ttcagggttg actctgaaag aggaaagcgt aatatacgcc acctcgcgac
     6241 agtgagctga aaaccgcgtc gcaactgctc tttaacaatt tatcagacaa tctgtgtggg
     6301 cactcgaaga tacggattct tgacgtcgca agacgaaaaa tgaataccaa gtctca
//
LOCUS       DAHHTG010000192         6283 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.195, whole genome shotgun sequence.
ACCESSION   DAHHTG010000192 DAHHTG010000000
VERSION     DAHHTG010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6283)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6283)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6283
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.195"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            complement(45..1004)
                     /gene="rfaC"
                     /locus_tag="NBI74_004251"
     CDS             complement(45..1004)
                     /gene="rfaC"
                     /locus_tag="NBI74_004251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase RfaC"
                     /protein_id="HCD1934487.1"
                     /translation="MRVLIVKTSSMGDVLHTLPALTDAQQAIPGIKFDWVVEEGFAQI
                     PSWHAAVERVIPVAIRRWRKAWFSAPIKAERKAFREALQAENYDAVIDAQGLVKSATL
                     VTRLAHGVKHGMDWQTAREPLASLFYNRKHHIAKQQHAVERTRELFAKSLGYSKPQTQ
                     GDYAIAQHFLTNLPTDAGEYAVFLHATTRDDKHWPEEHWRELIGLLADSGIRIKLPWG
                     APHEEERAKRLAEGFAYVEVLPKMSLEGVARVLAGAKFVVSVDTGLSHLTAALDRPNI
                     TVYGPTDPGLIGGYGKNQMVCRAPRENLINLNSQAVLEKLSSL"
     gene            complement(1008..2054)
                     /gene="rfaF"
                     /locus_tag="NBI74_004252"
     CDS             complement(1008..2054)
                     /gene="rfaF"
                     /locus_tag="NBI74_004252"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-heptose--LPS heptosyltransferase RfaF"
                     /protein_id="HCD1934488.1"
                     /translation="MKILVIGPSWVGDMMMSQSLYRTLQARYPQAIIDVMAPAWCRPL
                     LSRMPEVNEAIPMPLGHGALEIGERRKLGHSLREKRYDRAYVLPNSFKSALVPFFAGI
                     PHRTGWRGEMRYGLLNDVRVLDKEAWPLMVERYVALAYDKGIMRTAQDLPQPLLWPQL
                     QVSEGEKSYTCNQFSLSSERPMIGFCPGAEFGPAKRWPHYHYAELAKQLIDEGYQVVL
                     FGSAKDHEAGNEILAALNTEQQAWCRNLAGETQLDQAVILIAACKAIVTNDSGLMHVA
                     AALNRPLVALYGPSSPDFTPPLSHKARVIRLITGYHKVRKGDAAEGYHQSLIDITPQR
                     VLEELNALLLQEEA"
     gene            complement(2064..2996)
                     /gene="rfaD"
                     /locus_tag="NBI74_004253"
     CDS             complement(2064..2996)
                     /gene="rfaD"
                     /locus_tag="NBI74_004253"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HCD1934489.1"
                     /translation="MIIVTGGAGFIGSNIVKALNDKGITDILVVDNLKDGTKFVNLVD
                     LDIADYMDKEDFLIQIMAGEEFGDVEAIFHEGACSSTTEWDGKYMMDNNYQYSKELLH
                     YCLEREIPFLYASSAATYGGRTSDFIESREYEKPLNVYGYSKFLFDEYVRQILPEANS
                     QIVGFRYFNVYGPREGHKGSMASVAFHLNTQLNNGESPKLFEGSENFKRDFVYVGDVA
                     DVNLWFLENGVSGIFNLGTGRAESFQAVADATLAYHKKGQIEYIPFPDKLKGRYQAFT
                     QADLTNLRAAGYDKPFKTVAEGVTEYMAWLNRDA"
     gene            3210..4406
                     /gene="kbl"
                     /locus_tag="NBI74_004254"
     CDS             3210..4406
                     /gene="kbl"
                     /locus_tag="NBI74_004254"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HCD1934490.1"
                     /translation="MRGEFYQQLTNDLETARAEGLFKEERIITSAQQADITVADGSHV
                     INFCANNYLGLANHPELIAAAKAGMDSHGFGMASVRFICGTQDSHKELEQKLAAFLGM
                     EDAILYSSCFDANGGLFETLLSAEDAIISDALNHASIIDGVRLCKAKRYRYANNDMHE
                     LEARLKEAREAGARHVLIATDGVFSMDGVIANLKGVCDLADKYDALVMVDDSHAVGFV
                     GENGRGSHEYCDVMGRVDIITGTLGKALGGASGGYTAARKEVVEWLRQRSRPYLFSNS
                     LAPAIVAASIKVLEMVEAGSELRDRLWANARQFREQMSAAGFTLAGADHAIIPVMLGD
                     AVVAQKFARELQKEGIYVTGFFYPVVPKGQARIRTQMSAAHTPEQITRAVEAFTRIGK
                     QLGVIA"
     gene            4416..5441
                     /gene="tdh"
                     /locus_tag="NBI74_004255"
     CDS             4416..5441
                     /gene="tdh"
                     /locus_tag="NBI74_004255"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HCD1934491.1"
                     /translation="MKALSKLKAEEGIWMTDVPVPELGHNDLLIKIRKTAICGTDVHI
                     YNWDEWSQKTIPVPMVVGHEYVGEVVGIGQEVKGFKIGDRVSGEGHITCGHCRNCRGG
                     RTHLCRNTIGVGVNRPGCFAEYLVIPAFNAFKIPDNISDDLASIFDPFGNAVHTALSF
                     DLVGEDVLVSGAGPIGIMAAAVAKHVGARNVVITDVNEYRLELARKMGITRAVNVAKE
                     NLNDVMAELGMTEGFDVGLEMSGAPPAFRTMLDTMNHGGRIAMLGIPPSDMSIDWTKV
                     IFKGLFIKGIYGREMFETWYKMAALIQSGLDLSPIITHRFSIDDFQKGFDAMRSGQSG
                     KVILSWD"
     gene            5680..>6283
                     /gene="waaH"
                     /locus_tag="NBI74_004256"
     CDS             5680..>6283
                     /gene="waaH"
                     /locus_tag="NBI74_004256"
                     /EC_number="2.4.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucuronate:LPS(HepIII) glycosyltransferase"
                     /protein_id="HCD1934492.1"
                     /translation="MMNSTNKLSVIIPLYNAGDDFRTCMESLITQTWTALEIIIINDG
                     STDNSVEIAKHYAENYPHVRLLHQANAGASVARNRGIEVATGKYVAFVDADDEVYPTM
                     YETLMTMALEDDLDVAQCNADWCFRETGETWQSIPTDRLRSTGVLTGPDWLRMGLSSR
                     RWTHVVWMGVYRRDVIVKNNIKFIAGLHHQDIVWTTEFMFN"
BASE COUNT         1469 a         1556 c         1638 g         1620 t
ORIGIN      
        1 ggtttcaatg aatgaagttt aaaggatgtt agcatgtttt acctttataa tgatgataac
       61 ttttccaaaa ctgcttgact gttgaggtta attaaatttt ctcttggagc cctacaaacc
      121 atctgattct tcccataccc accaattaat cccggatcgg taggtccata aaccgtgata
      181 ttgggtctat ccagtgccgc cgttaaatgg cttaaccccg tatccaccga cactacaaat
      241 ttagccccgg ccagtacgcg ggcaacgcct tccagactca tcttcggcaa tacttcaaca
      301 taagcaaatc cttccgccag tcgtttcgcc cgttcttcct catgcggcgc gccccacgga
      361 agtttaatcc gtattcctga atcagccagt aaaccaatca attctcgcca gtgttcttcc
      421 ggccagtgtt tatcatctcg ggttgttgca tgaagaaata cggcatattc gccagcatct
      481 gtaggcaggt tcgtcaggaa atgctgtgcg atagcataat cgccctgggt ttgtggtttg
      541 ctatagccca gacttttggc aaacagttcg cgggtgcgtt ctacggcgtg ctgctgtttt
      601 gcaatatgat gtttacgatt gtaaaacagg ctggctaacg gttcgcgagc ggtttgccag
      661 tccattccat gctttacgcc atgcgccaga cgcgtcacca gcgtcgcgct ttttaccagc
      721 ccctgagcgt cgataactgc gtcatagttc tctgcttgta gcgcttcacg aaacgctttg
      781 cgttcagctt ttatgggggc cgagaaccag gctttacgcc agcgacgtat tgccacagga
      841 ataactcgct caacggcagc gtgccaggaa ggaatctgtg cgaacccttc ttccaccacc
      901 cagtcaaact taatccctgg gattgcctgc tgggcatcag tgagtgcggg cagcgtatgg
      961 agaacatcgc ccatcgacga tgttttaacg atcagaaccc gcatccgtca ggcttcctct
     1021 tgtaacaata gcgcgttgag ttcttccagt acgcgctggg gagtaatgtc gatcaagctc
     1081 tggtgataac cctccgccgc gtcaccttta cgcactttgt gatagccggt aatcaggcgg
     1141 atcacgcgtg ctttatggga tagcggtggt gtgaagtccg ggctactcgg accatacagg
     1201 gcaaccagcg gacgattgag cgccgccgca acgtgcatca ggccagaatc gttagtaaca
     1261 atggctttac aggctgcaat caggataacc gcttgatcaa gctgtgtttc ccccgccagg
     1321 ttccgacacc atgcctgctg ctcggtattc aaagcggcaa gaatctcatt gcccgcttca
     1381 tgatctttcg ccgagccaaa cagaaccacc tgataacctt catcaatcag ctgctttgcc
     1441 agctccgcat agtggtagtg tggccagcgt tttgccggac caaactccgc acccgggcaa
     1501 aaaccaatca tcggacgttc tgatgagagc gaaaattgat tacaggtata tgatttttca
     1561 ccttcgctca cctgcaactg cggccataac aatggctgcg gcagatcttg tgctgtacgc
     1621 ataatgcctt tgtcataggc cagcgcgaca tagcgttcca ccattagcgg ccaggcttct
     1681 ttatcgagca cgcgtacatc gttgagtaaa ccgtagcgca tctcgccgcg ccagccggtg
     1741 cgatgaggaa tacctgcgaa gaaaggcact aatgcggatt tgaaggagtt aggtaagacg
     1801 taggcgcggt cgtagcgctt ttcacgcagg ctatgaccca gtttgcggcg ttcgccgatt
     1861 tccagcgctc cgtgaccgag aggcataggg atagcttcgt taacttccgg catccgcgat
     1921 aataatggac ggcaccatgc cggtgccatc acgtcgatta tcgcctgggg atagcgcgcc
     1981 tggagcgtgc gatagagact ttgcgacatc atcatgtcgc caacccaaga cgggccgatc
     2041 accagtattt tcatgcagag ctcttatgcg tcacgattca gccaggccat gtattccgtt
     2101 acgccttcgg caacggtttt gaacggttta tcgtaacccg ccgcgcgcag attcgtcaga
     2161 tctgcctgag tgaacgcctg gtagcggcct ttcagtttat ccgggaacgg aatgtattcg
     2221 atttggcctt tcttgtgata agcaagcgta gcatctgcta ctgcctggaa ggattccgca
     2281 cgaccggtgc cgaggttgaa gatgccggaa acgccatttt ccaggaacca caggtttaca
     2341 tctgccacgt cgcccacata gacgaagtca cgtttaaagt tctcgctacc ttcgaacagc
     2401 ttcggtgatt caccgttatt aagctgggtg ttgagatgga aagcgacgct cgccatactg
     2461 cctttatggc cttcacgagg tccataaacg ttgaaataac ggaagccaac aatctgcgag
     2521 ttcgcttcag gcaggatttg gcgaacatat tcatcaaaca ggaattttga gtaaccgtag
     2581 acattcaacg gtttttcata ttcgcgggat tcaataaagt cggaggtgcg tccaccgtaa
     2641 gtggctgcgg aagaggcata caggaacggg atttcgcgct ccagacagta gtgcagcagc
     2701 tctttggagt attgatagtt gttatccatc atatacttgc cgtcccactc ggtggtggaa
     2761 gagcacgcgc cttcgtggaa aatcgcttcg acatcgccga actcttcgcc agccataatc
     2821 tggatcagga agtcttcctt atccatatag tccgcgatat ccagatccac caggttcaca
     2881 aacttggtgc cgtctttcag gttgtccacc accagaatat cggtgatgcc tttatcattc
     2941 agggccttaa cgatgttgct gccgataaag cccgcgccgc cggtaacgat gatcataact
     3001 gtaaccttcg aattatggag tcagagacaa tctcagacac gaatacttct atcatatcac
     3061 tacacggcgt tggcttcagc cattcgccaa aagtgagatt gatggacgtt atttatcctg
     3121 aattttgcag aagtgttaac gcgtcatctc gtcgcgacct agaagtttgg gtaatatgtg
     3181 ctggaatttg ccctgtctgg agaatcgcaa tgcgtggaga attttatcag cagttaacca
     3241 acgatctgga aaccgcacgg gcggaagggt tgtttaaaga agagcgcatt attacgtctg
     3301 cgcagcaagc agatatcact gtggctgatg gaagccacgt tattaacttt tgcgccaata
     3361 actatctcgg actggcaaat catccagagc tgattgcggc ggcaaaagcg ggaatggact
     3421 ctcatggttt tgggatggct tcggtgcgct ttatctgcgg cacccaggat agccataaag
     3481 agcttgaaca aaaactggcg gcattcctgg ggatggaaga tgcgattctc tactcttcct
     3541 gctttgatgc taacggcggc ctgtttgaaa cgcttctgag cgcggaggac gccattatct
     3601 ccgacgcact gaaccacgcg tctattattg atggtgtgcg tctgtgtaaa gctaaacgct
     3661 atcgttatgc caacaacgat atgcatgagc tggaagctcg tctgaaagaa gcgcgtgaag
     3721 ccggtgcgcg tcatgtgctg attgccaccg atggtgtgtt ctcgatggac ggcgtgattg
     3781 ccaacctgaa gggcgtttgc gatctggcag ataaatacga tgccctggtg atggtagacg
     3841 actcccacgc ggtcggtttt gtcggtgaaa atggtcgtgg ttcccatgaa tactgcgatg
     3901 tgatggggcg ggtcgatatt atcaccggta cgcttggtaa agcgttgggc ggggcttctg
     3961 gtggttacac cgcagcgcgt aaagaagtgg ttgagtggct gcgtcagcgt tctcgtcctt
     4021 acctgttctc caactcactg gcacctgcca ttgtggctgc ttccattaaa gtgctggaga
     4081 tggtcgaagc gggcagtgag cttcgtgacc gtctgtgggc gaacgcgcgt cagttccgtg
     4141 agcagatgtc ggcggcgggc tttaccctgg cgggtgccga tcacgccatt attccggtca
     4201 tgcttggcga tgctgtagtg gcgcaaaaat ttgcccgtga actgcaaaaa gagggcattt
     4261 acgtcaccgg tttcttctat ccggtcgttc ctaaaggtca ggcgcgtatt cgtacccaga
     4321 tgtctgcggc gcatacccct gagcaaatta cgcgtgcagt agaagcattc acgcgtattg
     4381 gtaaacaact gggcgttatc gcctgagggt gtgagatgaa agcgttatcc aaactgaaag
     4441 cggaagaggg catctggatg accgacgttc ctgtaccgga actcgggcat aacgatctgc
     4501 tgattaaaat ccgtaaaaca gccatctgcg ggactgacgt tcacatctat aactgggatg
     4561 agtggtcgca aaaaaccatc ccggtgccga tggtcgtggg ccatgaatat gtcggtgaag
     4621 tggtaggtat tggtcaggaa gtgaaaggct tcaagattgg cgatcgcgtt tctggcgaag
     4681 gccatatcac ctgcggtcac tgtcgcaact gtcgtggcgg tcgtacccat ctgtgccgca
     4741 acacgatcgg cgtcggtgtt aaccgtccgg gctgcttcgc cgagtatctg gtgattccgg
     4801 cgttcaacgc cttcaaaatc ccagacaata tctccgacga cctggcttcg atttttgatc
     4861 ccttcggtaa cgccgtgcat accgcgctgt cgttcgatct ggttggcgag gatgtgctgg
     4921 tctctggtgc aggtccgata ggtattatgg ctgcggcggt ggcgaaacac gttggtgcac
     4981 gcaatgtggt gatcactgat gttaacgaat accgccttga gctggcacgc aaaatgggta
     5041 tcacccgtgc ggttaacgtc gctaaagaaa accttaatga tgtgatggca gaactgggca
     5101 tgaccgaagg ctttgatgtc ggtctggaaa tgtccggtgc gccgccagcg tttcgtacca
     5161 tgctcgacac catgaaccac ggcggtcgta ttgcgatgct gggtattcca ccgtccgata
     5221 tgtctatcga ctggaccaaa gtaatcttta aaggcttgtt cattaaaggt atttacggtc
     5281 gtgagatgtt tgaaacctgg tacaagatgg cggcgctgat tcagtctggc ctcgatctct
     5341 cgccgatcat tacccatcgt ttctctatcg atgatttcca gaagggcttt gacgctatgc
     5401 gttcgggcca gtccgggaaa gttattctga gctgggatta acacgaacaa gggctggtat
     5461 tccagccctt ttgcctgagg ataatccgtt aaatatgtaa aatcctgtca gtgtaataaa
     5521 gagttcgtaa ttgcactgat ctcttatata gctgctctca ttatctctct accctgaagt
     5581 gactctctca ccggtaaaaa taatatctca caggcttaat agtttcttaa tacaaagcct
     5641 gtaaaacgtc aggataactt cagaggtcgt cggtaattta tgatgaacag caccaataaa
     5701 cttagtgtta ttatcccgtt atataatgcg ggcgatgatt tccgcacttg tatggaatct
     5761 ttaattacgc aaacatggac tgctctggaa atcattatta ttaacgatgg ttcaacggat
     5821 aattctgttg aaatagcaaa gcattacgca gaaaactatc cgcacgttcg tttgttgcat
     5881 caggcgaatg ctggcgcatc ggtggcgcgt aatcgtggga ttgaagtggc aacgggaaaa
     5941 tatgtcgctt ttgtcgatgc tgacgatgag gtctatccca ccatgtacga aacgctgatg
     6001 accatggcgt tagaggatga cctcgacgtg gcgcagtgca acgctgattg gtgttttcgt
     6061 gaaacgggtg aaacctggca atccatcccc accgatcgcc ttcgctcaac cggtgtatta
     6121 accggtccgg actggctgcg gatgggactt tcttcccgcc gttggacaca cgtggtctgg
     6181 atgggggttt atcgccgcga tgttattgtt aaaaataaca ttaaatttat tgccggatta
     6241 catcatcagg atattgtctg gacaacagaa tttatgttta acg
//
LOCUS       DAHHTG010000193         6135 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.196, whole genome shotgun sequence.
ACCESSION   DAHHTG010000193 DAHHTG010000000
VERSION     DAHHTG010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6135)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6135)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6135
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.196"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            complement(74..994)
                     /gene="speB"
                     /locus_tag="NBI74_004257"
     CDS             complement(74..994)
                     /gene="speB"
                     /locus_tag="NBI74_004257"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HCD1934493.1"
                     /translation="MSTLGHQYDNSLVSNAFGFLRLPMNFQPYDSDADWVITGVPFDM
                     ATSGRAGGRHGPAAIRQVSTNLAWEHNRFPWNFDMRERLNVVDCGDLVYAFGDAREMS
                     EKLQAHAEKLLAAGKRMLSFGGDHFVTLPLLRAHAKHFGKMALVHFDAHTDTYANGCE
                     FDHGTMFYTAPKEGLIDPNHSVQIGIRTEFDKDNGFTVLDACQVNDRSVDDIIAQVKQ
                     IVGDMPVYLTFDIDCLDPAFAPGTGTPVIGGLTSDRAIKLVRGLKDLNIVGMDVVEVA
                     PAYDQSEITALAAATLALEMLYIQAAKKGE"
     gene            complement(1130..1861)
                     /locus_tag="NBI74_004258"
     CDS             complement(1130..1861)
                     /locus_tag="NBI74_004258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1934494.1"
                     /translation="MKKWKLRSALIALAVLLAGCSSNAQYNSSASGNVGTAWGGDVHS
                     TVQGVSAERAWRDPAEMIVISYSTNVPSGYDRVYSIRINELEYAIRDGNFNSLPITRV
                     YDSSNNEPRYIVHARIGMNYQLYIRNYSRNTNYEIVATVDGLDVLNGKQGSLNNNGYI
                     VNAGDSLAIKGFRKDKHTEAAFQFANVADSYAANSAQGDVHNTGVIGFAAFELQGPAQ
                     NALPPCSGQAFPADNNGYAPPPCRK"
     gene            complement(2007..3983)
                     /gene="speA"
                     /locus_tag="NBI74_004259"
     CDS             complement(2007..3983)
                     /gene="speA"
                     /locus_tag="NBI74_004259"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HCD1934495.1"
                     /translation="MSDDMSMGLPSSAGEHGVLRSMQEVAMSSQEASKMLRTYNIAWW
                     GNNYYDVNELGHISVCPDPDVPEARVDLAQLVKTREAQGQRLPALFCFPQILQHRLRS
                     INAAFKRARESYGYNGDYFLVYPIKVNQHRRVIESLIHSGEPLGLEAGSKAELMAVLA
                     HAGMTRSVIVCNGYKDREYIRLALIGEKMGHKVYLVIEKMSEIAIVLDEAERLNVVPR
                     LGVRARLASQGSGKWQSSGGEKSKFGLAATQVLQLVETLREAGRLDSLQLLHFHLGSQ
                     MANIRDIATGVRESARFYVELHKLGVNIQCFDVGGGLGVDYEGTRSQSDCSVNYGLNE
                     YANNIIWAIGDACEENGLPHPTVITESGRAVTAHHTVLVSNIIGVERNEYTVPTAPAE
                     DAPRALQSMWETWQEMHEPGTRRSLREWLHDSQMDLHDIHIGYSSGTFSLQERAWAEQ
                     LYLSMCHEVQKQLDPQNRAHRPIIDELQERMADKMYVNFSLFQSMPDAWGIDQLFPVL
                     PLEGLDQVPERRAVLLDITCDSDGAIDHYIDGDGIATTMPMPEYDPENPPMLGFFMVG
                     AYQEILGNMHNLFGDTEAVDVFVFPDGSVEVELSDEGDTVADMLQYVQLDPKTLLTQF
                     RDQVKKTDLDAELQQQFLEEFEAGLYGYTYLEDE"
     gene            complement(3992..4123)
                     /gene="yqgB"
                     /locus_tag="NBI74_004260"
     CDS             complement(3992..4123)
                     /gene="yqgB"
                     /locus_tag="NBI74_004260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response protein YqgB"
                     /protein_id="HCD1934496.1"
                     /translation="MKKKPVAQLERQHSLLENPCAYGLLSQFQAAIVVNCFTLNKII"
     gene            4259..4474
                     /locus_tag="NBI74_004261"
     CDS             4259..4474
                     /locus_tag="NBI74_004261"
                     /inference="COORDINATES: protein motif:HMM:NF037550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1934497.1"
                     /translation="MGITSAGMQSRDAECGERIFTRTVRQVKQQTTVHYFVSPPRPPV
                     KTNPQAKTLFSTRLEVATRKKRRVLFI"
     gene            4778..5932
                     /gene="metK"
                     /locus_tag="NBI74_004262"
     CDS             4778..5932
                     /gene="metK"
                     /locus_tag="NBI74_004262"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HCD1934498.1"
                     /translation="MAKHLFTSESVSEGHPDKIADQISDAVLDAILEQDPKARVACET
                     YVKTGMVLVGGEITTSAWVDIEEITRNTVREIGYVHSDMGFDANSCAVLSAIGKQSPD
                     INQGVDRADPLEQGAGDQGLMFGYATNETDVLMPAPITYAHRLVQRQAEVRKNGTLPW
                     LRPDAKSQVTFQYDDGKIVGIDAVVLSTQHSEEIDQKSLQEAVMEEIIKPILPAEWLT
                     SATKFFINPTGRFVIGGPMGDCGLTGRKIIVDTYGGMARHGGGAFSGKDPSKVDRSAA
                     YAARYVAKNIVAAGLADRCEIQVSYAIGVAEPTSIMVETFGTEKVPSEQLTLLVREFF
                     DLRPYGLIQMLDLLHPIYKETAAYGHFGREHFPWEKTDKAQLLRDAAGLK"
BASE COUNT         1554 a         1664 c         1496 g         1421 t
ORIGIN      
        1 tgtaggtcgg ataaggcgtt cacgccgcat ccgacattaa tgacacatca cccgtgcgcc
       61 tcttatctgg cgcttactcg ccctttttcg ccgcctgaat atacagcatc tccagcgcca
      121 gcgtcgccgc agccagagcg gtgatttccg actgatcgta tgccggagcc acttccacta
      181 cgtccatccc aacgatgttg agatctttca ggccgcgtac cagtttaatg gcgcgatcgg
      241 aggtcaggcc gccaattacc ggcgtaccgg tgcctggtgc aaaagcagga tccaggcagt
      301 cgatatcaaa ggtcaggtaa accggcatat cacccacaat ctgtttcacc tgggcgataa
      361 tgtcatccac gctgcgatcg ttcacctggc aggcgtccag cacggtaaag ccgttgtctt
      421 tatcaaactc ggtacgaata ccaatctgca cggaatgatt cgggtcgatc agaccttctt
      481 tcggcgcggt atagaacatg gtgccgtggt caaattcaca accgttcgca taggtatcgg
      541 tgtgggcgtc aaagtgtacc aacgccattt tgccgaaatg cttcgcatga gcgcgcagca
      601 gcggcagcgt aacaaagtgg tcaccaccga aggagagcat acgcttaccg gcagccagca
      661 gcttctcggc gtgcgcctgc agcttctcgc tcatctcacg ggcatcgccg aaggcatata
      721 ccagatcacc gcagtccacg acgttcaggc gctcacgcat gtcgaaattc cacgggaagc
      781 ggttgtgttc ccaggccaga ttcgtcgaaa cctgacggat cgctgccgga ccgtgacgac
      841 cacccgcacg accagaagtg gccatatcga acggcacgcc agtaatcacc cagtcagcat
      901 cgctgtcata cggctggaag ttcatcggca ggcgtaaaaa accaaaggcg ttggaaacca
      961 gtgagttatc gtattgatga cctaaggtgc tcatggatgg actcctttta caaagtcgat
     1021 ataaaaaaac ccttccgcgt cgttaggccc gacgaggaag ggttggattt gcgaaacaca
     1081 agtttgtgct aattatcgcc ttaaatgggc gaggcatcaa gcgaggttgc tatttccgac
     1141 acggtggtgg cgcatagcca ttgttatctg ccggaaacgc ttgtccagag cacggcggca
     1201 gcgcgttttg cgccgggcct tgtaattcaa aggcggcaaa gccgattaca ccggtattat
     1261 gcacatcgcc ttgcgccgaa ttggctgcgt aggaatcagc aacgttggcg aactgaaaag
     1321 ccgcttcggt atgtttatct ttgcggaatc ctttaatcgc taacgaatct ccggcattca
     1381 caatataacc gttgttattc agcgaacctt gcttgccgtt cagcacatcc aggccatcaa
     1441 ccgtagcaac gatctcgtag ttggtgttac gactgtaatt acggatatat aattgataat
     1501 tcattccgat tctggcatgg acaatatagc gtggttcatt attggatgaa tcataaaccc
     1561 gcgtaatagg tagagaatta aaattaccat cgcgaatagc atattccagt tcgttaatac
     1621 gaatactata aacgcggtcg tagccggaag gaacattagt cgaataacta ataacgatca
     1681 tctctgccgg atcgcgccag gctcgttcag cagatacgcc ctgaaccgtt gagtgcacat
     1741 ccccacccca ggcggtgccg acattacccg atgcactgct attatactgc gcattactgg
     1801 aacaaccggc cagcagcacg gctaatgcta tcaacgcgct acgtaatttc cattttttca
     1861 taaccgttcc ctctgcattt aattaatgca gcgattgtag ggagattatt taataagaaa
     1921 agcagctaaa ttatttttaa gaatatataa aaagaaagcg ggccgattta cgtaaataaa
     1981 tcgccccgcc agcggaataa tcttaattat tcatcttcaa gataggtata accgtacaaa
     2041 cctgcctcga actcttcaag gaactgttgt tgcagttcag catcaagatc ggttttcttc
     2101 acctgatcgc ggaactgagt taacagcgtt ttcggatcga gctgcacata ttgcagcatg
     2161 tccgccacgg tatcgccttc gtcagacagt tctacctcta cgctaccatc agggaagacg
     2221 aacacgtcaa ccgcttcggt atcaccgaac aggttgtgca tgttgccaag gatctcctga
     2281 tatgcgccga ccataaagaa accgagcatc ggcggattct ccggatcgta ctccggcata
     2341 ggcattgtcg tggcaatacc gtcaccatca atatagtggt cgatagcacc gtctgagtca
     2401 caggtaatat ccaacagcac agcgcggcgt tccggcactt gatccagccc ttccagcggc
     2461 agaaccggga acaactggtc gatcccccac gcatccggca tcgactggaa cagcgagaag
     2521 ttgacgtaca ttttgtccgc catacgttcc tgcagctcgt cgataatcgg acgatgagca
     2581 cggttttgcg gatccagctg cttttgcact tcatggcaca tgctcaaata aagctgctca
     2641 gcccatgcgc gttcttgcag gctaaaggtg ccggaagagt agccgatatg aatatcgtgc
     2701 agatccatct ggctgtcgtg taaccattca cgcagagaac ggcgagttcc cggttcgtgc
     2761 atttcctgcc aggtttccca catgctttgc agggcgcgcg gtgcgtcttc tgcaggcgcg
     2821 gtcggcaccg tgtattcgtt acgttccacg ccgatgatat tcgacaccag cacggtgtga
     2881 tgcgcagtca ccgcacgacc cgattcggtg attaccgtcg gatgtggcag accattttct
     2941 tcacacgcat caccaatcgc ccagatgatg ttgttggcgt attcattgag gccgtagttt
     3001 acggagcagt cagactgcga acgggtgcct tcatagtcca cgcccagacc gccgccgacg
     3061 tcgaagcact gaatattgac gcccagcttg tgcagttcca catagaaacg cgcggattca
     3121 cgaacgcctg tcgcgatatc gcgaatattc gccatctgcg aaccgaggtg gaagtgcagc
     3181 agttgcaggc tgtcgagacg cccggcttca cgcagcgttt caaccagttg cagtacctga
     3241 gtcgccgcca ggccaaactt cgatttttcc ccgccggagg actgccattt acccgagcct
     3301 tgcgacgcca gacgtgcacg cacgcccaga cgaggaacga cattcagacg ttctgcttca
     3361 tccagcacaa tggcaatttc tgacatcttc tcaatgacca gatagacctt gtggcccatc
     3421 ttctcgccaa ttaatgccag gcggatatat tcgcggtcct tataaccgtt gcagacgatg
     3481 acgctacggg tcatgccagc atgtgccagc accgccatca actcagcttt ggaaccggct
     3541 tccaggccca gcggttcgcc tgaatgaatc agggactcaa tcacgcggcg gtgctggtta
     3601 actttgatcg gataaacgag gaagtaatcg ccgttatagc cgtaggattc ccgcgctcgt
     3661 ttgaacgcgg cgttaatgga acgcaaacgg tgctgcagga tctgtgggaa acagaacagt
     3721 gcaggcagac gctgaccctg tgcttcgcga gttttcacta actgcgcgag atcgacgcga
     3781 gcttccggga cgtccgggtc cgggcacacg ctgatgtggc ccagctcgtt aacgtcatag
     3841 tagttattgc cccaccaggc aatattgtaa gtacgcagca tcttgctggc ttcctgggag
     3901 ctcattgcaa cctcctgcat ggagcgtagt acaccgtgtt cgcccgctga cgaaggcaaa
     3961 cccatagaca tgtcgtcaga catagcgaac ctcaaattat tttattaagt gtaaaacagt
     4021 taacgactat cgcagcctgg aactgcgata acaacccata agcacatgga ttttccagca
     4081 gtgaatgctg acgctccaac tgcgcaaccg gtttcttttt cataacatta ttaagcacat
     4141 aaccgaacgt aagtgtgaca gttcggcgaa accacgagaa aactcttgtt tttacaagag
     4201 cgcccttgtt cagtcctcag tagctgtaac cagctcttga atcctgagaa gcgccgagat
     4261 gggtataaca tcggcaggta tgcaaagcag agatgcagag tgcggggaac gaatcttcac
     4321 cagaacggtg agacaggtta agcagcagac aacggttcat tatttcgtat cacctccacg
     4381 gccgcctgtt aagacgaacc cacaagccaa aactctgttt tcaacccggc tggaagtggc
     4441 aacacgaaag aaacgtcgtg tgctttttat ttaagccgcg cgccgcgttt tataccccac
     4501 aatggcagaa aattgcaaaa gataaatacg cagaatgcca gcattgtcag gaaaaatttc
     4561 cagccacgtt tttaacagaa tgagacacga ttcaaaaaaa agtggaaatt gggtgaagaa
     4621 ctgacctaaa atagccatcc agatgttaat ccatccatac cgattaacac tcagactgcc
     4681 agtgttttta acctgcagag tcgtggtagg atccgctacc acagaaaatc cacacaacag
     4741 tttgagctaa ccaaattctc tttaggtgat attaaatatg gcaaaacacc tttttacgtc
     4801 cgagtccgtc tctgaagggc atcctgacaa aattgctgac caaatctctg atgccgtttt
     4861 agacgcgatc ctcgaacagg atccgaaagc acgcgttgct tgcgaaacct acgtaaaaac
     4921 cggcatggtt ttagttggcg gcgaaatcac caccagcgcc tgggtagaca tcgaagagat
     4981 cacccgtaac accgttcgcg aaattggcta tgtacattcc gacatgggct ttgacgctaa
     5041 ctcctgtgcg gttctgagcg ccatcggtaa acagtctcct gacatcaacc agggcgttga
     5101 ccgtgccgat ccgctggaac agggcgcagg cgaccagggc ctgatgtttg gctacgcaac
     5161 taatgaaacc gacgtgctga tgccagcacc tatcacttat gcacaccgtc tggtacagcg
     5221 tcaggctgaa gtgcgtaaaa acggcactct gccgtggctg cgcccggacg cgaaaagcca
     5281 ggtgactttc cagtatgacg acggcaaaat cgttggcatt gatgctgtcg tgctttccac
     5341 tcagcactct gaagagatcg accagaaatc gctgcaagaa gcggtaatgg aagagatcat
     5401 caagccgatt ctgcccgctg aatggctgac ttctgccacc aaattcttca ttaacccgac
     5461 cggtcgtttc gttatcggtg gcccgatggg tgactgcggt ctgactggtc gtaaaatcat
     5521 cgttgatacc tacggcggca tggcgcgtca tggtggcggt gcgttctccg gtaaagatcc
     5581 atcaaaagtg gaccgttccg cagcctacgc agcacgttat gtcgcgaaaa acattgttgc
     5641 tgctggcctg gccgatcgtt gtgaaattca ggtttcctac gcaatcggcg tggctgaacc
     5701 gacttccatc atggtagaaa ctttcggtac tgagaaagtg ccttctgaac aactgactct
     5761 gctggtacgt gaattcttcg acctgcgccc atacggtctg attcagatgc tggatctgct
     5821 gcacccgatc tacaaagaaa ccgcagcata cggtcacttt ggtcgtgaac atttcccgtg
     5881 ggaaaaaacc gacaaagcgc agctgctgcg tgatgctgcc ggtctgaagt aatctttctt
     5941 cacctgcttt caaaggccag cctcgcgctg gcctttttct tttacctcac cgcggtggac
     6001 aggcattcac gccgcatccg gcaaaaaaac cgcccgcaca ataacatcat tcttcctcaa
     6061 cacgttttac cgcaaattat catcactact gaaaccgatt acaccaacca cgccagccaa
     6121 atatttgtaa tattt
//
LOCUS       DAHHTG010000194         6099 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.197, whole genome shotgun sequence.
ACCESSION   DAHHTG010000194 DAHHTG010000000
VERSION     DAHHTG010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6099)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6099)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6099
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.197"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            114..298
                     /locus_tag="NBI74_004263"
                     /pseudo
     CDS             114..298
                     /locus_tag="NBI74_004263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165315.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            259..378
                     /locus_tag="NBI74_004264"
     CDS             259..378
                     /locus_tag="NBI74_004264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HCD1934499.1"
                     /translation="MPLSYTGYHHFIMMSLMSGEHNTFALSLQDESPGLVPAG"
     gene            413..755
                     /locus_tag="NBI74_004265"
                     /pseudo
     CDS             413..755
                     /locus_tag="NBI74_004265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311542.2"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
     gene            complement(1122..1388)
                     /gene="minE"
                     /locus_tag="NBI74_004266"
     CDS             complement(1122..1388)
                     /gene="minE"
                     /locus_tag="NBI74_004266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HCD1934500.1"
                     /translation="MALLDFFLSRKKNTANIAKERLQIIVAERRRSDAEPHYLPQLRK
                     DILEVICKYVQIDPEMVTVQLEQKDGDISILELNVTLPEAEELK"
     gene            complement(1392..2204)
                     /gene="minD"
                     /locus_tag="NBI74_004267"
     CDS             complement(1392..2204)
                     /gene="minD"
                     /locus_tag="NBI74_004267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020801817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HCD1934501.1"
                     /translation="MARIIVVTSGKGGVGKTTSSAAIATGLAQKGKKTVVIDFDIGLR
                     NLDLIMGCERRVVYDFVNVIQGDATLNQALIKDKRTENLYILPASQTRDKDALTREGV
                     AKVLDDLKAMDFEFIVCDSPAGIETGALMALYFADEAIITTNPEVSSVRDSDRILGIL
                     ASKSRRAENGEEPIKEHLLLTRYNPGRVSRGDMLSMEDVLEILRIKLVGVIPEDQSVL
                     RASNQGEPVILDINADAGKAYADTVERLLGEERPFRFIEEEKKGFLKRLFGG"
     gene            complement(2228..2923)
                     /gene="minC"
                     /locus_tag="NBI74_004268"
     CDS             complement(2228..2923)
                     /gene="minC"
                     /locus_tag="NBI74_004268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HCD1934502.1"
                     /translation="MSNTPIELKGSSFTLSVVHLHEAEPKVIHQALEDKIAQAPAFLK
                     HAPVVLNVSALEDPVNWSAMHKAVSATGLRVIGVSGCKDAQLKAEIEKMGLPILTEGK
                     EKSPRPAPAPQAPAQNTTPVTKTRLIDTPVRSGQRIYAPQCDLIVTSHVSAGAELIAD
                     GNIHVYGMMRGRALAGASGDRETQIFCTNLMAELVSIAGEYWLSDQIPAEFYGKAARL
                     QLVENALTVQPLN"
     gene            3443..3811
                     /locus_tag="NBI74_004269"
     CDS             3443..3811
                     /locus_tag="NBI74_004269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1934503.1"
                     /translation="MNMMRIFYIGLSGVGMMFSSMASGNDAGGLQSPACGVVCDPYIC
                     VSSDGISPELTRKYLGEKAAENLQSLQGYDPSEFTFSNGVFCDVKEKLCRDDRYFGVD
                     GKRSGKINQTTTKMLFMCRE"
     gene            complement(3914..4315)
                     /locus_tag="NBI74_004270"
     CDS             complement(3914..4315)
                     /locus_tag="NBI74_004270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPC domain-containing protein"
                     /protein_id="HCD1934504.1"
                     /translation="MKIKGISKAVLLLALLTSTSFAAGKNVNVEFRKGHSSAQYSGEI
                     KGYDYDTYTFYAKKGQKVHVSISNEGADTYLFGPGIDDSVDLSRYSPELDSHGQYSLP
                     ASGKYELRVLQTRNDARKNKTKKYNVDIQIK"
     gene            4556..4849
                     /locus_tag="NBI74_004271"
     CDS             4556..4849
                     /locus_tag="NBI74_004271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgL domain-containing protein"
                     /protein_id="HCD1934505.1"
                     /translation="MFCVIYRSSKRDQTYLYVEKKDDFSRVPEELMKGFGQPQLAMIL
                     PLDGRKKLVNADIEKVKQALTEQGYYLQLPPPPEDLLKQHLSVMGQKTDDTNK"
     gene            4921..5580
                     /locus_tag="NBI74_004272"
     CDS             4921..5580
                     /locus_tag="NBI74_004272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HCD1934506.1"
                     /translation="MYQHHNWQGALLDYPVSKVVCVGSNYAKHIKEMGSAVPEEPVLF
                     IKPETALCDLRQPLAIPSDFGSVHHEVELAVLIGATLRQATEEHVRKAIAGYGVALDL
                     TLRDVQGKMKKAGQPWEKAKAFDNSCPLSGFIPAAEFTGDPQNTTLGLSVNGEQRQQG
                     TTADMIHKIVPLIAYMSKFFTLKVGDVVLTGTPDGVGPLQSGDELTVTFDGHSLTTRV
                     L"
     gene            5672..>6099
                     /locus_tag="NBI74_004273"
     CDS             5672..>6099
                     /locus_tag="NBI74_004273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309703.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgN family cysteine cluster protein"
                     /protein_id="HCD1934507.1"
                     /translation="MSDVPFWQSKTLDEMSDAEWESLCDGCGQCCLHKLMDEDTDEIY
                     FTNVACRQLNIKTCQCRNYERRFEFEPDCIKLTRENLPTFEWLPMTCAYRLLAEGKGL
                     PAWHPLLTGSKAAMHGERISVRHIAVKESEVIDWQDHILN"
BASE COUNT         1573 a         1338 c         1487 g         1701 t
ORIGIN      
        1 agaaataact cattgattga caatattttt attcaagaag tgtcattggc tgttaataca
       61 atgttgtaaa ggtaagataa tctgatttat caatattatt gtgtgattgt tatgtgagca
      121 gaagatattc attagcaacg attacattag tcattttatt ttgtggacgg cctcattgtc
      181 gaaaaataag cgtacgacag tattatcaga aaagagtgtt ttttatccaa caacacttca
      241 gcgcactgcg tttaaaaaat gcctctttct tatacgggat atcatcattt catcatgatg
      301 tctttgatga gcggtgaaca caatacattt gcgctgtctc ttcaggatga atcccctggt
      361 ctggtgcctg cgggctgatg ttgcagcaga gctaaggtca cttagacgct actatcattt
      421 atccaatgca tggaatcgaa atcagtcgat acccgcagta tatatcgtga actgggcgca
      481 acgctgagct acaacatgcg tctggggaac ggtatggaaa ttgaaccctg gctgaaggcg
      541 gctgtgcgca aagaatttgt cgatgataac cgggtgaagg tgaatagtga cggtaatttt
      601 gtcaatgatt tgtcgggcag acgtggaata taccaggcag gtattaaagc ctcattcagc
      661 agtatgttaa gcgggcatct cggggtgggt ataaccgtgg tgccggtgtg gagtccccgt
      721 ggaatgcggt ggccggtgtg aactggccgt tctgaccatc aacgattaac tgcgtttcgg
      781 cgctgttttc gtttacaggg tgctaaaagg gaaaattctg gggcaaaaaa agcccgccag
      841 ttacggcggg aaacctcatc ctattggaga acaatgaata atgaaattgc gggttatcat
      901 ctccctggat acccatgcta acaataaggt tatttactca accaggcgta aacattttgt
      961 tctgtgcgtg ggaacagcct taaggtgtaa agggggggga aatagcaatg agtagtatca
     1021 gcaagaatac tcgccgcttt accacaacgt ggatgagagg gatgaaaaac tcaaggcaga
     1081 gatgactctg ccttgaagat aaatgcgctt ttacaggggg cttatttcag ctcttctgct
     1141 tccggtaagg tcacgttcag ctcaagaata gaaatatcgc catctttttg ctcaagctgt
     1201 acggttacca tctcaggatc aatttgtacg tatttacaaa tgacctcaag aatatcttta
     1261 cgcaactgcg gcagataatg cggttctgca tcgctgcgac ggcgttcagc aacaataatc
     1321 tgcaaccgtt cttttgcaat gttggctgtg tttttcttcc gcgagagaaa gaaatcgagt
     1381 aatgccataa cttatcctcc gaacaagcgt ttgaggaagc ctttcttctc ttcttcaatg
     1441 aagcggaaag gacgttcttc tcccaacaga cgttctacgg tatctgcgta ggctttaccc
     1501 gcatcggcgt taatgtcgag aatgaccggt tcaccctggt tagaggcgcg caatactgat
     1561 tgatcctctg ggatcacgcc gacgagtttg atgcgcagga tctccagcac atcttccatg
     1621 ctcagcatgt cacctctgct tacgcggcct gggttatagc gcgttaacag caggtgctct
     1681 ttaatcggct cttcgccatt ttctgcgcgg cgtgatttcg acgccagaat gcctaaaata
     1741 cggtcagagt cgcgtactga ggagacttcc gggttggtgg taataatggc ttcgtctgca
     1801 aaatagagtg ccattaacgc accggtttca atccctgccg gggagtcaca aacgataaat
     1861 tcaaaatcca tcgctttcag atcatcaaga actttggcga ccccttcacg ggtgagggca
     1921 tctttatcgc gagtttgcga tgccggaaga atatagagat tttcagtacg tttatcttta
     1981 attaacgcct ggtttagcgt tgcatcgccc tgaatgacgt tgacgaaatc gtaaacgacc
     2041 cggcgttcac aacccataat caggtcgaga ttacgcaggc cgatatcaaa atctatcacg
     2101 acagttttct ttcccttctg ggccaaacca gtggcgatgg ccgcgctgga ggttgtctta
     2161 ccaacacccc ctttgcccga agtaacaaca ataatgcgtg ccatagaaat tccttgttaa
     2221 aaagggatca atttaacggt tgaacggtca aagcgttttc gactaactgc agtcgtgccg
     2281 ctttgccata aaattctgct gggatttgat cactcagcca gtattcacct gcgatggaca
     2341 ccagttccgc catcaggttc gtacaaaata tttgcgtttc ccggtcacca cttgcccctg
     2401 ccagcgcacg accgcgcatc atgccataga catgaatgtt accatcggca atcaattcgg
     2461 cccctgcgct aacgtggctt gtaacaatca gatcacattg tggagcataa atacgctgac
     2521 cggaacgcac tggggtatca attaaacgcg tttttgtgac cggcgttgta ttttgcgctg
     2581 gagcctgcgg tgcgggggct ggacgcggcg atttttcctt tccttccgtc aggataggca
     2641 gccccatctt ttcaatttcg gctttaagtt gcgcatcttt gcagccactt acgccaataa
     2701 cccgcaaacc agttgccgaa accgccttat gcatcgccga ccagtttacc gggtcttcca
     2761 gtgcactgac gttgagtaca acgggggcat gttttaaaaa tgcgggggcc tgagcgattt
     2821 tgtcttccag cgcctgatgg ataaccttag gttctgcctc atgcagatga accacagata
     2881 aagtgaagct actgccttta agctcgattg gcgtgtttga catcctggcc ttactcaatt
     2941 agctattaat catcgccagc gcgcgatgat gttccgaaga ctataaggca tgttatagtc
     3001 tggattatat tgaggcaagt caccctccca tttattcaga gtaaaagtct attctgtgat
     3061 aaatggcgtt gattcatagc ttaaaaaata cccttgtcaa tcaacccatt gccgtcgtac
     3121 ttttgattgt tcttatttac gcttcctttt tccgcaccct aatcgtgaca gcatcttcag
     3181 acctgtttaa atatccagta ttgaggcgag tgtatttttt caccacgcca ggctttctta
     3241 actttccatt ttttgcagca tcattaaaga acagataccc gttatcttaa tatccgccgc
     3301 agaattgctc tatcatagaa accgcagaag acgtctgata cttctgtcag gtcatcgtaa
     3361 ctcgtccagg tcagcggttt tgattacgga ttgatgatga aatgagcata agtgttgtac
     3421 ggttggatta tcaggatgtg ctatgaatat gatgcgtatt ttttatatcg gattgtcagg
     3481 tgtggggatg atgttctcat caatggcgtc tggaaatgac gctggcgggc ttcaatctcc
     3541 tgcgtgtggc gttgtttgcg acccgtatat ttgtgtgagt tcagatggca tttccccaga
     3601 gttaacaagg aaatatctcg gcgaaaaagc cgctgaaaac ttacaatcat tacaaggcta
     3661 cgatcccagc gaatttacat tctctaacgg tgtattttgc gatgttaaag aaaaattatg
     3721 tcgtgatgat cgttattttg gtgtggatgg taagcggagt ggaaaaatca atcaaaccac
     3781 cacaaagatg ttatttatgt gtcgtgagtg atgtcttgtt tacattatgt tctattcgtc
     3841 attaacctct taagcgacag agaggagtgt ggcgttaacg tttatgacgg ggagagtccc
     3901 cgtcggttga catttactta atttgaatat cgacgttata ttttttagtt ttgtttttac
     3961 gggcgtcgtt tcgggtttgc agcactcgca gctcatattt gccagaagca gggagtgagt
     4021 attgtccgtg actatcgagt tctggggaat atctggataa atcaacagaa tcatcgattc
     4081 ctggcccgaa gaggtaagta tcagcgcctt cattagagat actcacatgc actttctgac
     4141 cttttttggc atagaaggta tatgtatcgt aatcgtatcc ctttatttcg ccggagtact
     4201 gagcgctgct atggcctttt ctgaactcaa cattgacatt cttacccgca gcaaagctcg
     4261 tggaagttag cagtgcaagc aacaatacag ccttactgat gcccttaatt ttcattggta
     4321 gtctcctgtt cagaatgagt acgatgaact gttataatat aacaatcctt cacgggagaa
     4381 ttccagtggc tttcctgtgt tcggttaaat cctggcgcgg catttttgcc gggggattac
     4441 ttataaaggg ggcatggact gaacaaaccg tagaaatcta cagctggctg gcttgtggcg
     4501 cgggtttcat tgatgttagc ttatgccaaa acctggcatc ttaaagagta aaagtatgtt
     4561 ttgtgtgatt tatcgaagca gcaaacgtga ccagacctat ttatatgtcg aaaaaaaaga
     4621 cgatttttcg cgtgttcctg aggaactgat gaaaggtttt ggtcagcctc agttagcgat
     4681 gattcttccg ctggatgggc ggaagaaact ggtcaatgcc gatattgaaa aggttaaaca
     4741 ggcattaacc gagcaaggtt actatttgca gttaccgcca ccacccgaag atttgctgaa
     4801 gcaacatctt tccgtcatgg ggcagaaaac agacgacact aacaaataac cgatatccgg
     4861 cggtggcatt atctttgtcg gcgcgggttt tcatatccac gataaggtga ggggaacgtt
     4921 atgtatcaac atcacaactg gcaaggtgcg ctgctggatt atccggtgag taaagtagtc
     4981 tgtgttggca gtaactatgc caaacatatt aaagagatgg gcagcgcagt acccgaagag
     5041 ccagtgctgt ttattaaacc agaaacggca ctgtgcgatc tgcggcagcc attggcgatc
     5101 ccatccgatt tcggttcagt tcatcatgaa gtcgaactgg cggtgttgat tggcgcgacg
     5161 ctgcgtcagg ctacggaaga gcatgtccgc aaagccattg ccggttacgg cgtggcgctc
     5221 gatctgacat tgcgtgatgt tcagggaaaa atgaagaaag ccgggcagcc gtgggaaaag
     5281 gccaaagcgt ttgataactc ttgtccgctt tccgggttta ttcccgcggc ggaatttacc
     5341 ggcgacccgc aaaatacaac gctgggcctg agcgtaaacg gcgagcaacg ccagcaaggt
     5401 acgactgcgg acatgatcca taaaatcgtt ccgctgatcg cttatatgag caagtttttt
     5461 accctcaagg tcggtgacgt tgtgctgaca ggcacgcctg atggtgtcgg cccgttgcaa
     5521 agcggtgatg agctgacagt cactttcgat gggcattctt tgacaactcg cgttttgtaa
     5581 tactttttgc cgcctgaaag cggcggcaaa acttgcatcg ctgtgccaga ctggttataa
     5641 ggtgcgtttt aacgtaatgg cggaacacct gatgagcgat gtacctttct ggcaaagtaa
     5701 aaccctggac gaaatgagcg atgcagaatg ggagtcgttg tgtgatggtt gcggtcagtg
     5761 ttgcctgcat aaactgatgg atgaagacac cgacgaaatc tacttcacta acgtcgcctg
     5821 tcgccagctc aatattaaaa cctgtcaatg tcggaactac gaacgtcgtt tcgagtttga
     5881 acccgactgc attaaattaa cccgtgaaaa tctgccaaca ttcgaatggc tgccaatgac
     5941 ctgcgcttat cgtttgctgg cggaaggtaa aggtttacct gcgtggcatc cgctacttac
     6001 tggttcgaaa gcggcaatgc atggtgaacg tatctctgtg cgtcatatcg cagtgaaaga
     6061 atcagaagtg attgactggc aggatcatat cttaaataa
//
LOCUS       DAHHTG010000195         6084 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.198, whole genome shotgun sequence.
ACCESSION   DAHHTG010000195 DAHHTG010000000
VERSION     DAHHTG010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6084)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6084)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6084
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.198"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            complement(151..741)
                     /gene="cobO"
                     /locus_tag="NBI74_004274"
     CDS             complement(151..741)
                     /gene="cobO"
                     /locus_tag="NBI74_004274"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HCD1934508.1"
                     /translation="MSDERYQQRQQRVKEKVDARVAQAQDERGIIIVFTGNGKGKTTA
                     AFGTATRAVGHGKKVGVVQFIKGTWPNGERNLLEPHGVEFQVMATGFTWDTQNRESDT
                     AACREVWQHAKRMLADSSLDMVLLDELTYMVAYDYLPLEDVIQALNERPHQQTVIITG
                     RGCHRDILELADTVSELRPIKHAFDAGVKAQIGIDY"
     gene            complement(738..1496)
                     /locus_tag="NBI74_004275"
     CDS             complement(738..1496)
                     /locus_tag="NBI74_004275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciK family oxidoreductase"
                     /protein_id="HCD1934509.1"
                     /translation="MHYQPKQDLLNDRIILVTGASDGIGREAAMTYARYGATVILLGR
                     NEEKLRQVASHINEETGRQPQWFILDLLTCTSEDCQQLAQRIAVNYPRLDGVLHNAGL
                     LGDVCPMSEQDPQVWQDVMQVNVNATFMLTQALLPLLLKSDAGSLVFTSSSVGRQGRA
                     NWGAYAASKFATEGMMQVLADEYQQRLRVNCINPGGTRTAMRASAFPTEDPQKLKTPA
                     DIMPLYLWLMGDDSRRKTGMTFDAQPGRKPGISQ"
     gene            1716..2765
                     /gene="sohB"
                     /locus_tag="NBI74_004276"
     CDS             1716..2765
                     /gene="sohB"
                     /locus_tag="NBI74_004276"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease SohB"
                     /protein_id="HCD1934510.1"
                     /translation="MELLSEYGLFLAKIVTVVLAIAAIAAIIVNVAQRNKRQRGELQV
                     NNLSEQYKEMKEELAAALMDTHQQKQWHKAQKKKHKQEAKAAKAKAKLGEVATDSKPR
                     VWVLDFKGSMDAHEVNSLREEITAVLAAFKPQDQVVLRLESPGGMVHGYGLAASQLQR
                     LRDKNIPLTVTVDKVAASGGYMMACVADKIVSAPFAIVGSIGVVAQMPNFNRFLKSKD
                     IDIELHTAGQYKRTLTLLGENTEEGREKFREELNETHQLFKDFVKRMRPSLDIEQVAT
                     GEHWYGQQAVEKGLVDEINTSDEVILSLMEGREVVNVRYMQRKRLIDRFTGSAAESAD
                     RLLLRWWQRGQKPLM"
     gene            complement(2801..3052)
                     /locus_tag="NBI74_004277"
     CDS             complement(2801..3052)
                     /locus_tag="NBI74_004277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciN family protein"
                     /protein_id="HCD1934511.1"
                     /translation="MNKETQPIDRETLLKEANKIIREHEDTLAGIEATGVTQRNGVLV
                     FTGNYFLDEQGLPTAKSTAVFNMFKHLAHVLSEKYHLVD"
     gene            3432..6029
                     /gene="topA"
                     /locus_tag="NBI74_004278"
     CDS             3432..6029
                     /gene="topA"
                     /locus_tag="NBI74_004278"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000513601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HCD1934512.1"
                     /translation="MGKALVIVESPAKAKTINKYLGSDYVVKSSVGHIRDLPTSGSAA
                     KKSADSTSTKTAKKPKKDERGALVNRMGVDPWHNWEAHYEVLPGKEKVVSELKQLAEK
                     ADHIYLATDLDREGEAIAWHLREVIGGDDARYSRVVFNEITKNAIRQAFDKPGELNID
                     RVNAQQARRFMDRVVGYMVSPLLWKKIARGLSAGRVQSVAVRLVVEREREIKAFVPEE
                     FWEVDASTTTPSGEALALQVTHQNDKPFRPVNKEQTQAAVSLLEKARYSVLEREDKPT
                     TSKPGAPFITSTLQQAASTRLGFGVKKTMMMAQRLYEAGYITYMRTDSTNLSQDAVNM
                     VRGYISDNFGKKYLPESPNQYASKENSQEAHEAIRPSDVNVMAESLKDMEADAQKLYQ
                     LIWRQFVACQMTPAKYDSTTLTVGAGDFRLKARGRILRFDGWTKVMPALRKGDEDRIL
                     PAVDKGDALTLVELTPAQHFTKPPARFSEASLVKELEKRGIGRPSTYASIISTIQDRG
                     YVRVENRRFYAEKMGEIVTDRLEENFRELMNYDFTAQMENSLDQVANHEAEWKAVLDH
                     FFSDFTQQLDKAEKDPEEGGMRPNQMVLTSIDCPTCGRKMGIRTASTGVFLGCSGYAL
                     PPKERCKTTINLVPENEVLNVLEGEDAETNALRAKRRCPKCGTAMDSYLIDPKRKLHV
                     CGNNPTCDGYEIEEGEFRIKGYDGPIVECEKCGSEMHLKMGRFGKYMACTNEECKNTR
                     KILRNGEVAPPKEDPVPLPELPCEKSDAYFVLRDGAAGVFLAANTFPKSRETRAPLVE
                     ELYRFRDRLPEKLRYLADAPQQDPEGNKTMVRFSRKTKQQYVSSEKDGKATGWSAFYV
                     DGKWVDGKK"
BASE COUNT         1555 a         1445 c         1624 g         1460 t
ORIGIN      
        1 gtttattgaa gacgtcaatg cggataatga tatggaatat cccgcacact tgaataaaat
       61 tcaagctctt cttaattttg ccgtggctaa ttaagccatt cagtatctaa aattagcacc
      121 tcaagaggcg aacttttgag gtgctttcac ttagtaatca atccctatct gcgcttttac
      181 accggcatca aacgcatgtt tgatggggcg cagctcactt accgtatccg ccagttcaag
      241 aatatcccga tgacagcctc gacccgtgat aatcaccgtc tgttgatgtg gacgttcatt
      301 taacgcctga atcacgtctt ccagtggcaa atagtcatac gctaccatat atgtcagttc
      361 atcgagcaaa accatatcca gtgaggaatc agccagcatc cgctttgcat gttgccagac
      421 ttcgcggcag gcggcggtat cggactctcg gttttgtgta tcccaggtaa agcccgttgc
      481 cattacctga aactcaacgc cgtgtggctc cagcagattt cgttcaccat taggccaggt
      541 gcctttaata aactgcacga cgcccacttt ttttccgtga ccaactgcgc gtgttgccgt
      601 accaaatgcc gcggtggttt tgccttttcc attgccggta aagacgatga taataccgcg
      661 ttcatcctgg gcctgggcca cacgagcatc tactttttct ttcactcgct gctgacgctg
      721 ttggtagcgt tcatcactca ttgggaaatt cctggtttac ggcccggttg ggcgtcaaag
      781 gtcatgccgg ttttacggcg gctgtcatcg cccatcagcc agaggtagag cggcatgata
      841 tcagcggggg ttttaagttt ctgtggatct tcggtcggga aggcgctggc ccgcattgcg
      901 gtgcgcgtac cgcctgggtt aatgcagttg acacgcaggc gctgctgata ttcatcggcc
      961 agtacctgca tcatcccttc ggtggcaaat ttcgacgctg catatgcgcc ccagtttgca
     1021 cgcccctgac gtccaacgct tgatgaggta aagaccagtg aaccggcgtc cgatttgagt
     1081 aataaaggaa gcagtgcctg ggtgagcata aaggtggcat taacgttgac ctgcatgacg
     1141 tcctgccaga cttgcggatc ttgttcgctc attgggcaaa catcgccgag caatccggca
     1201 ttatgcaaaa caccatccag acgcggataa ttaacggcaa tgcgctgtgc cagttgttgg
     1261 caatcttcgg acgtgcaggt tagcaaatcg agaataaacc actgtggctg acgtccagtt
     1321 tcttcgttta tgtggctggc tacctgacgt aatttttctt cattacggcc caaaagaatc
     1381 actgtcgcac catagcgtgc atacgtcatc gctgcttcac gaccaattcc atcgctggct
     1441 cccgtcacca gaataatgcg atcattgagt aaatcttgtt ttggctggta atgcatggct
     1501 actcctcaac gacgttgtct gtcgcgctgc cagtgtactt tatgactttg gggctttatg
     1561 cctgaaacag aagggcattt caatcagcct ggggcgataa caccgcaaaa tggatacttt
     1621 gtcatacttt cgctgcaata acatctctgc gagacggcta aacatgcctg ttgtaaactg
     1681 tgagccaaag cgttgtttaa ccaaggtggg gactcgtgga attgttgtct gaatatggtt
     1741 tgtttttggc gaaaatcgtt accgttgtgc tagcgattgc ggcgattgcc gccattattg
     1801 tcaatgttgc tcaacgtaat aaacgccagc gtggcgagtt acaggtcaac aatctcagcg
     1861 aacagtataa ggagatgaaa gaagaactgg ccgcggcgct gatggatact catcagcaaa
     1921 aacagtggca caaagcgcag aagaaaaagc acaagcaaga agcgaaagcc gcaaaagcga
     1981 aagctaaact gggcgaggta gcaactgaca gtaaaccccg cgtctgggtg ctggatttta
     2041 aaggcagcat ggacgcccat gaagtgaact cgctacgtga agagataacg gcggtactcg
     2101 cagcattcaa accgcaggat caggttgtgc tccgtctgga aagccctggt ggcatggtgc
     2161 atggttacgg gttggcggct tcgcagttgc agcgtctgcg tgataaaaac attcctttaa
     2221 ctgttacggt agacaaagtc gcggccagcg gcggttacat gatggcctgt gtggcggaca
     2281 aaattgtttc cgcaccgttt gctattgtgg gttccattgg cgtggtggcg caaatgccta
     2341 actttaaccg cttcctgaaa agcaaagata ttgatatcga actgcacacc gccgggcagt
     2401 ataagcgcac gctgacgttg ctgggtgaaa ataccgaaga agggcgggag aaattccgcg
     2461 aagagttgaa cgaaacgcat cagttgttta aagattttgt gaagcgtatg cgtccgtctc
     2521 tggatattga acaggtggca acgggtgaac actggtacgg acaacaggca gtagagaaag
     2581 gcctggttga tgaaatcaac accagtgatg aagttattct tagcctgatg gaaggccggg
     2641 aagtggtcaa tgtacgctat atgcagcgta aacgactcat tgaccgattc accggcagcg
     2701 cggcagagag cgccgatcga ttgttgttac gttggtggca gcgtgggcaa aagccgttga
     2761 tgtaaaagac aaacgcgagg ataagacctc gcgttttgct ttaatcaacc aggtgatatt
     2821 tttctgaaag cacatgggcc aggtgtttga acatattaaa caccgcggtg cttttggctg
     2881 ttggtaatcc ttgttcatct aaaaagtagt tgccggtaaa taccagcacg ccattacgct
     2941 gcgtgacgcc ggtggcttca atccctgcga gcgtatcctc atgctcacga atgattttgt
     3001 tggcctcttt caacagcgtt tcgcggtcga tgggttgtgt ctctttgttc attatttact
     3061 ccttaaacaa ggacattagt ctacgccagg catggcttgc agacaaatat accacgctgg
     3121 tggcaagagc gccttactgg caactttgga ttttgcatgc taataaagtt gcgtatcgga
     3181 ttttatcagg tacagtgtga cgctttcgtc aatctggcaa tagatttgct tgacattcga
     3241 ccaaaattcc gtcgtgctat agcgcctgta ggccaagacc tgttaactca gtcacctgaa
     3301 ttttcgtgaa cagagtcacg acaaggggtt gatatccgca gagagcgagt ccatatcggt
     3361 aactcgtggc cagtggaagg tttatcaacg tgcgacgcat tcctggaaga atcaaattag
     3421 gtaaggtgaa tatgggtaaa gctcttgtca tcgttgagtc cccggcaaaa gccaaaacga
     3481 tcaacaagta tctgggtagt gactacgtgg tgaaatccag cgtcggtcac atccgcgatt
     3541 tgccgaccag tggctcagct gccaaaaaga gtgccgactc tacctccacc aagacggcta
     3601 aaaagcctaa aaaggatgaa cgtggcgctc tcgtcaaccg tatgggggtt gacccgtggc
     3661 acaattggga ggcgcactat gaagtgttgc ctggtaaaga gaaggtcgtc tctgaactga
     3721 aacaactggc tgaaaaagcc gaccacatct atctcgcaac cgaccttgac cgcgaagggg
     3781 aagccattgc atggcacctg cgggaagtga ttggtggtga tgatgcgcgc tatagccgag
     3841 tggtgtttaa cgaaattact aaaaacgcga tccgccaggc atttgacaaa ccgggtgaac
     3901 tgaatattga tcgtgttaat gcccagcagg cgcgtcgctt tatggaccgc gtggtggggt
     3961 atatggtttc gccgcttcta tggaaaaaga tcgctcgtgg tctgtctgcc ggtcgtgtgc
     4021 agtcggtggc ggtccgcctg gtggtcgagc gtgagcgtga aattaaagcg ttcgtaccgg
     4081 aagagttctg ggaagtcgat gccagcacga ccacgccatc tggtgaagcg ttggcgttgc
     4141 aggtgactca tcagaacgac aaaccgttcc gtccggtcaa caaagaacaa actcaggctg
     4201 cggtaagtct gctggaaaaa gcgcgctaca gcgtgctgga acgtgaagac aaaccgacaa
     4261 ccagtaaacc tggcgctcct tttattacct ctacgctgca acaagctgcc agcacccgtc
     4321 ttggttttgg cgtgaaaaaa accatgatga tggcgcagcg tttgtatgaa gcaggctata
     4381 tcacttacat gcgtaccgac tccaccaacc tgagtcagga cgcggtaaat atggttcgcg
     4441 gttatatcag cgataatttt ggtaagaaat atctgccgga aagtccgaat cagtacgcca
     4501 gcaaagaaaa ctcacaggaa gcgcacgaag cgattcgtcc ttctgacgtc aatgtgatgg
     4561 cggaatcgtt gaaggatatg gaagcagatg cgcagaaact gtaccagtta atctggcgtc
     4621 agtttgtagc ctgccagatg accccagcga aatatgactc cacgacgctg accgttggtg
     4681 cgggcgattt ccgcctgaaa gcacgcggtc gtattttgcg ctttgatggc tggacgaaag
     4741 tgatgcctgc actgcgtaaa ggcgatgaag atcgtatctt accggcagtc gataaaggcg
     4801 atgctctgac gctcgttgaa ctgacaccag cccagcactt taccaagcct ccagcccgtt
     4861 tcagtgaagc atcgctggtc aaagagctgg aaaaacgcgg tatcggtcgc ccttctacct
     4921 atgcgtcgat catttcgacc attcaggatc gtggctatgt gcgagtagaa aatcgtcgtt
     4981 tctatgcgga aaaaatgggc gaaatcgtca ccgatcgcct ggaagagaat ttccgcgagt
     5041 taatgaacta cgacttcacc gcgcagatgg aaaacagcct cgaccaggtg gcaaatcacg
     5101 aagcagagtg gaaagctgta ctggatcact tcttctcgga tttcacccag cagttagata
     5161 aagctgaaaa agatccggaa gagggtggta tgcgcccgaa ccagatggtt ctgaccagca
     5221 ttgactgccc gacttgtggt cgcaaaatgg ggattcgcac agcgagcacc ggggtattcc
     5281 ttggctgttc tggctatgcg ctgccgccga aagagcgttg caaaaccacc attaacctgg
     5341 tgccggaaaa cgaagtgctg aacgtgctgg aaggcgaaga cgctgaaacc aatgcgttgc
     5401 gcgcaaaacg tcgttgcccg aaatgcggta cggcgatgga cagctatctc atcgatccga
     5461 aacgtaagtt gcatgtctgt ggtaataacc caacctgcga cggttacgag atcgaagagg
     5521 gcgaattccg cattaaaggt tatgacggcc cgatcgttga gtgtgaaaaa tgtggctctg
     5581 aaatgcacct gaaaatgggg cgtttcggta aatacatggc ctgcaccaac gaagagtgta
     5641 aaaacacacg taagatttta cgtaacggcg aagtggcacc accgaaagaa gatccggtgc
     5701 cattaccgga gctgccgtgc gaaaaatcag atgcctattt cgtgttgcgt gacggtgctg
     5761 ccggtgtgtt cctggcggcc aataccttcc cgaaatcgcg tgaaacacgt gcgccactgg
     5821 tggaagagct ttatcgcttc cgcgaccgtc tgccggaaaa actgcgttat ctggccgatg
     5881 cgccacagca ggatccggaa ggtaataaga ccatggttcg ctttagccgt aaaaccaaac
     5941 agcaatatgt ctcttcggaa aaagacggaa aggcgactgg ctggtcagca ttttatgttg
     6001 atggcaaatg ggttgatggg aaaaaataac ctttaattct gtcgggtttt tatcaacaaa
     6061 gggtcgcgaa agcggccctt tttt
//
LOCUS       DAHHTG010000196         6084 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.199, whole genome shotgun sequence.
ACCESSION   DAHHTG010000196 DAHHTG010000000
VERSION     DAHHTG010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6084)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6084)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6084
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.199"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            284..865
                     /gene="mdaB"
                     /locus_tag="NBI74_004279"
     CDS             284..865
                     /gene="mdaB"
                     /locus_tag="NBI74_004279"
                     /EC_number="1.6.5.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase MdaB"
                     /protein_id="HCD1934513.1"
                     /translation="MSNILIINGAKKFAHSNGQLNDTLTEVADGTLRDLGHDVRIVRA
                     DSDYDVKAEVQNFLWADVVIWQMPGWWMGAPWTVKKYIDDVFTEGHGTLYASDGRTRK
                     DPSKKYGSGGLVQGKKYMLSLTWNAPMEAFTEKDQFFHGVGVDGVYLPFHKANQFLGM
                     EPLPTFIANDVIKMPDVPRYTEEYRKHLVEIFG"
     gene            896..1210
                     /locus_tag="NBI74_004280"
     CDS             896..1210
                     /locus_tag="NBI74_004280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HCD1934514.1"
                     /translation="MITVIAEIRTRPGQHHRQAVLDQFAKIVPTVLKEEGCHGYAPMV
                     DCAAGVSFQSMAPDSIVMIEQWESIAHLEAHLQTPHMKAYSEAVKGDVLEMNIRILQP
                     GL"
     gene            complement(1255..2142)
                     /locus_tag="NBI74_004281"
     CDS             complement(1255..2142)
                     /locus_tag="NBI74_004281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HCD1934515.1"
                     /translation="MIQKDKARVDIFGERFRTRASQLTPGLRVVASYINEHREVVLEQ
                     TAMEIAATLNTSDATVIRAIQALGFAGLRDLKRTLEQWLGPALSSSEKMSTTVSNLTS
                     DVNTAIDFVLEGHLYTCNVLSEPENRHALAQAVALLVQARQVAIFGIGASGILADYTA
                     RLFNRIGLPATALNRTGIGLAEQLIALQRGDVLIMMAQKSAHREGLTTLREARRLGIP
                     VILLTNALDSRFSKDASIVIHVPRGDEKGKTPLHGTVLLCLEMIVWSVASAVPQRAVK
                     TIKRINDFHRGLKTGRKNG"
     gene            complement(2139..3086)
                     /locus_tag="NBI74_004282"
     CDS             complement(2139..3086)
                     /locus_tag="NBI74_004282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-siderophore ABC transporter
                     substrate-binding protein"
                     /protein_id="HCD1934516.1"
                     /translation="MRLFFSLLILLSFFSRATEPVQVFTDDLGRKVTVPAHPKRIVSL
                     HDLDITIPLIELGVPPVASHGRTRPDGSHFIRSGALLTGVDFDNSSIAFIGTADIDIE
                     AIVAAKPDLIITEPTRNTPIEQLEKIAPTVSIDHLKGGAPEIYRKLAELTGTQSKLAI
                     LERRYQAQINALKATLDSQKITVSVIQANQGKINVMHSYHSLGRVLRDAGFRFPPLIE
                     SIPEGGRMDVSAERLPELDADFVFATWRGDTGGKPQDELAAMEKVMPGWCQFLTACRS
                     GRYVLISREEAISNSFASLGLMAAQIQSQIAGRPLPEAK"
     gene            complement(3097..4134)
                     /locus_tag="NBI74_004283"
     CDS             complement(3097..4134)
                     /locus_tag="NBI74_004283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311941.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HCD1934517.1"
                     /translation="MNVGLRPLRVGKFSTLVRPKNLLLLGGLFLLAVGILIFGLMQGS
                     FSVPASEVGRALFAPENVSTDARYIVQDIRLPRVIMALLCGAMLGMAGAAMQSIARNG
                     LADPGLIGVKEGCSVAVLWLIFQFPMLGMFWRPVAGLAGGLLVALIVIFCARDISRPR
                     FVLIGIGVSWFFAAGIGVFMTTADVRDVQTALMWLSGSLHAANWMLVGISACWMLPAA
                     LLLLFTARTADIALLGHQVATGLGVNSSRLALLRVAAPIILTAVCVSCVGNIGFVGLI
                     APHISRFILRGGQTTLLLGSAVSGALLVILADSIGRLAFLPLQLPAGIIISLIGGPFF
                     LLLLWQRRNSF"
     gene            complement(4143..5126)
                     /locus_tag="NBI74_004284"
     CDS             complement(4143..5126)
                     /locus_tag="NBI74_004284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HCD1934518.1"
                     /translation="MMRTGLVTVIFLTLLLVGCVVYPGIGARFIAPQTVLQAFLHFDP
                     QNFDHNVIVRLRLPRLAAALLTGASLGVAGALLQAVIRNPLGEPHILGLNAGAALAVV
                     AASALGLAFPVGRPLLASTGGALLFLLILLLSSAGRSGVTPMKVTLCGVALSAFVSSI
                     TAAILILDEQTLLAMRTWLAGDLAGQDWATLGTSAWFSLGGFVLALYLAPSLNMLALG
                     DRMAQGLGVSVLRTRTFTLLAIALLCGAAVSIAGPIGFVGLLVPQIVRRLVSADLRVL
                     LPLSACVGALLLLLADIIARTLFTPHELATGVMTALVGAPVFVIMATRMFK"
     gene            complement(5123..5932)
                     /locus_tag="NBI74_004285"
     CDS             complement(5123..5932)
                     /locus_tag="NBI74_004285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCD1934519.1"
                     /translation="MAHNAKGQGLILDNVSAGYHKKIIVDGVSFSVPTEKMTVLVGAN
                     GCGKSTLLSTIARILQPMGGSILLDGKAIHEQPTKALSRRLGILPQSPLLPEGLTVYE
                     LVSRGRFPWQNFIRQWSDADEQAVEEALKLTGTQEFAHLPVEKLSGGQRQRCWIAMVL
                     AQKTPYILLDEPTTWLDLRYQVEILELLHDLTRHHGRTVVVVLHDLNFAVNYGDTLIF
                     LRQGKVVRVLNEGEHCTPELVKAVFDVDVHASINPLTGKPFFMPFRGVEKV"
BASE COUNT         1734 a         1633 c         1390 g         1327 t
ORIGIN      
        1 tccctttaat tgataagtat atgatatttg acatatatat tctccataat cattttttcc
       61 gaaaaactcc tgtatcattt gcataataca tctctcctgt gggagcttat gggaagttga
      121 tttaagtatt gtcattttca gaaaatattt atcccgcatc ggtgaatcag agaaagatca
      181 gacttttgct aaattcgcaa aagactttgc acattttgct aatttcacca taccgctctg
      241 tgacgtacta tagtcggcaa acgtctcacc ttgaggttaa aaaatgagca acatcctgat
      301 tatcaacggc gcgaaaaaat tcgcccactc caatggtcaa ctgaacgaca ccctgaccga
      361 agtcgcggat ggcacactgc gcgacctcgg gcatgatgtc cgcattgttc gcgccgacag
      421 cgactacgat gtcaaagcgg aagtacaaaa ctttctctgg gctgatgtgg tgatctggca
      481 gatgccaggc tggtggatgg gcgcgccgtg gacagtgaaa aaatatattg atgatgtatt
      541 caccgaaggt cacgggactc tgtatgccag cgatggtcgt acccgcaaag atccatcaaa
      601 aaaatacggt tccggcggcc tggtacaggg caaaaaatat atgctttcac tgacctggaa
      661 cgcaccaatg gaagccttca ccgaaaaaga tcagttcttc cacggcgttg gtgttgacgg
      721 tgtgtacctg ccgttccata aagcaaacca gttccttggc atggaaccgc tgccgacatt
      781 tatcgctaat gacgtgataa aaatgcctga tgttccccgc tatactgaag aatatcgcaa
      841 gcatcttgtg gaaatttttg gttaactaga gctctggctt tagaaggagt taaccatgat
      901 tacagtaatc gcagaaatcc gtactcgtcc tggtcaacat caccgtcagg cagtgttgga
      961 tcagtttgct aaaatcgttc caaccgtact gaaagaagaa ggttgccacg gctatgcgcc
     1021 aatggtggat tgcgctgctg gcgtgagttt ccagtctatg gcaccggatt ctatcgtgat
     1081 gattgagcag tgggaaagca tcgcgcatct tgaagcgcat ctgcaaaccc cgcacatgaa
     1141 agcatatagc gaagccgtaa aaggtgacgt gctggagatg aatattcgta tcctgcagcc
     1201 ggggctttga catttctaaa aagaggccgg tgctgctacc ggtctcagcc cagattagcc
     1261 attctttctt cccgttttca gtcctcgatg gaaatcgtta attcgcttaa tggttttaac
     1321 cgcacgctgc ggcactgcgg aagctaccga ccagacaatc atttccagac acaacaatac
     1381 cgtaccatgt agcggcgttt tccctttttc atccccgcgc ggaacatgaa tcacgatact
     1441 ggcgtctttg ctaaaacgtg aatccagagc gtttgtcagc aaaataacag ggatccccag
     1501 ccgcctcgcc tcacgaagcg ttgtcagccc ttcccggtgc gctgattttt gcgccatcat
     1561 gatcagaaca tcgccacgtt gaagcgcgat cagttgctcg gcaaggccaa tacccgtacg
     1621 gtttagtgcc gtggcaggca aacctatgcg gttgaatagt cgtgcggtat agtccgccag
     1681 aatacccgaa gcgccaatgc caaaaattgc gacttgccgt gcctgcacca acaacgctac
     1741 agcttgcgca agcgcgtggc ggttttcagg ctctgacagg acattacagg tatacaaatg
     1801 cccttcaaga acaaagtcga ttgcggtgtt aacatcgctc gtcaagttac tcaccgtcgt
     1861 agacatcttt tcgctggaac taagggctgg accaagccac tgttccagag tgcgtttcag
     1921 atcacgcagc ccggcaaacc ccagggcctg tatagccctg ataactgtgg catcggaagt
     1981 gttcaacgtg gccgcaattt ccatcgccgt ttgctcgagt accacttcac gatgctcatt
     2041 tatgtagctg gcgacaactc ttaacccagg cgttaactga ctcgcgcgag tgcgaaaacg
     2101 ctcaccaaaa atatcaaccc gcgctttatc tttctggatc atttagcctc cggtagaggt
     2161 cgccctgcaa tttgtgattg tatctgcgca gccatcagcc ccagagatgc aaaggagtta
     2221 gaaattgcct cttcacgaga aatcagcaca taacgcccgg aacgacaggc agtcagaaac
     2281 tgacaccatc ccggcatgac tttttccatt gctgccagct catcctgagg tttaccaccg
     2341 gtgtcaccgc gccaggttgc aaaaacaaaa tcggcatcca gttcaggtaa acgctccgcg
     2401 ctcacatcca ttcgcccacc ttcaggaatg ctctcaatca gcggcggaaa acgaaaacca
     2461 gcatcgcgta atacccgccc cagcgaatga tagctatgca tcacattaat ttttccctgg
     2521 tttgcctgaa tcaccgatac cgttattttt tggctgtcca gcgtcgcctt cagcgcattg
     2581 atttgtgcct gatagcggcg ttcaagtatc gccagcttcg actgcgttcc tgtaagttct
     2641 gccagcttgc gatagatttc tggcgcgccc cctttgagat gatcaatgct caccgtgggc
     2701 gcaatttttt caagttgttc aatgggcgta ttacgggtcg gttctgtgat gatcaggtca
     2761 ggctttgccg ccacaatcgc ttcaatatca atatcagcgg taccaataaa ggcgatcgat
     2821 gaattatcaa agtcgacacc tgtcaataac gcgccagatc gaatgaaatg gctaccgtca
     2881 ggccgagttc gcccgtggct ggcgacagga ggtacaccga gctcaatcag aggaatagtg
     2941 atatcaagat cgtgtaaaga gacaattctc tttggatgag caggaacagt gactttgcgc
     3001 cctaaatcat cagtaaatac ctgaacaggt tctgttgcac gggaaaaaaa tgacaacagt
     3061 atcagcagag aaaaaaataa acgcatataa ctcccgttaa aaactgttcc tgcgctgcca
     3121 gagcagtagc aaaaaaaatg gtccaccaat cagtgagata atgattccgg caggcagttg
     3181 caaaggcaaa aaagccaaac gaccaatgct atccgccaga ataactaaca acgcccccga
     3241 taccgcactc cccagcagta atgtcgtctg cccgccacgt agaataaaac gtgatatatg
     3301 gggtgctatc aatccgacaa aaccgatgtt accaacacac gaaacgcaga cggctgtcag
     3361 gattatgggt gcggcgacac gtaacaacgc caggcgagaa ctattcacgc ctaacccggt
     3421 tgccacctga tgaccaagca gagcgatatc cgcagtcctg gcggtgaaca gcaataaaag
     3481 agcggctggc aacatccaac aggctgaaat accgactaac atccagttag ctgcatgcag
     3541 gcttcccgat aaccacatca atgcagtttg tacatctcga acatctgcag tggtcataaa
     3601 tacaccgata ccggcggcga aaaaccagga aacaccaata ccaatcagaa caaatcgcgg
     3661 gcgggaaata tcgcgcgcac aaaaaatcac gattaaagcc accaacaggc caccagcaag
     3721 accagccact ggacgccaga acattcccag cattggaaac tggaaaatca gccacagcac
     3781 agccacgcta catccttctt tgacgccgat taaaccagga tcggcaaggc cgttacgtgc
     3841 aatggattgc attgcggccc ctgccatacc taacatggcc ccgcatagca atgccatgat
     3901 gactctcggc aagcgaatat cctgcacaat ataacgtgcg tctgtgctta cgttttctgg
     3961 agcaaacaac gctcgtccta cttccgatgc cggaacagag aaagaaccct gcatcagacc
     4021 gaatatcagt attccaaccg caagcaggaa cagcccacca agcaacagca gatttttagg
     4081 gcgtaccagt gtcgaaaact taccaacacg tagtgggcgt agcccgacgt tcattatccg
     4141 actcatttga acatcctcgt cgccataatg acaaaaacag gcgcaccaac taacgccgtc
     4201 atgacaccgg tcgctaactc atggggcgtg aaaagcgtcc gggcaataat atctgccagt
     4261 aataacaaaa gcgcgccgac acaagctgaa agagggagca gaacgcgcag atccgctgat
     4321 accagacgcc ggacaatctg cggtactagc aggccgacaa aaccaatggg gccagcaatc
     4381 gatacggctg cgccacagag caacgcgatc gcaagcaggg tgaatgttcg cgtgcggagt
     4441 acagagacgc ccagcccctg cgccatgcga tcgcccagtg ccaacatgtt aagggagggc
     4501 gcaaggtaaa gggctaacac aaatcccccc agagaaaacc aggcggacgt acccagcgtc
     4561 gcccaatcct gccccgctaa atcacctgct agccatgtcc gcatcgccag cagtgtttgt
     4621 tcgtcaagaa tcaggattgc agccgttatt gaggacacga acgccgatag cgccacgccg
     4681 cataaagtaa ctttcattgg cgttacccct gaacgccctg cggaggaaag cagcaatatc
     4741 aacaggaaca ataacgcgcc ccccgtagat gccagcagtg ggcgtccaac cggaaaggcc
     4801 agtccaagcg cactggcggc aacgacagca agagcggctc cggcatttaa gcctaaaata
     4861 tgtggctccc ccaatgggtt acgaatgaca gcctgcaaca aagcaccagc aacaccaaga
     4921 gaagcaccgg taagcaatgc tgccgctaaa cgcggcaaac ggagtcttac aattacgttg
     4981 tgatcgaagt tttgcggatc aaaatgaaga aatgcctgca acaccgtttg tggtgcaata
     5041 aatcgggcac caatccctgg atataccacg catcccacca gcagtaaggt gaggaaaatt
     5101 acagtaacca gccctgtgcg catcatactt tttctacacc acgaaacggc atgaagaacg
     5161 gctttcccgt cagaggattt attgatgcgt gaacatctac gtcaaaaaca gcttttacca
     5221 gctcaggcgt gcaatgctcc ccctcattca acacacgcac cactttcccc tggcgtaaaa
     5281 atatcaacgt atcgccataa ttgacggcaa aattcagatc atgcaacacc acgacgactg
     5341 tacgcccgtg atgacgcgtt aaatcatgta ataactcaag aatttcaacc tgataacgta
     5401 aatcaagcca ggttgtgggt tcatccagca ggatataagg cgtcttttgc gccaacacca
     5461 tcgcaatcca gcaacgctgg cgctgcccac cagaaagttt ttcaacaggt aaatgtgcaa
     5521 actcctgcgt acccgttaat ttcagggcct cttctaccgc ctgctcatcg gcgtcgctcc
     5581 attgacgtat gaaattttgc cagggaaaac gcccacgcga caccagttca taaacggtta
     5641 agccctccgg caataatggc gactgcggca agatcccaag tctacgggat aacgctttcg
     5701 ttggttgctc gtgaatcgct ttgccatcaa gcaatatcga accgcccatg ggttgcaaga
     5761 tacgtgcaat ggtgctgagc aacgtcgact ttccacagcc gtttgcccca accagaacgg
     5821 tcattttttc tgtgggaacg gagaaagaaa caccatcaac aatgattttc ttgtgatacc
     5881 cggcagaaac attgtcgagg atcagcccct gcccttttgc gttatgtgcc acgtgtacgc
     5941 caacttaatc aaagacaaac aaaaataaaa ctcattctcc tttgagagtt tgacgccaca
     6001 aaatgtagta gtcaagcagt taaaagccac acaaaaacaa ggtaaataac aggtaaaata
     6061 tttttataca taaaaataaa aaca
//
LOCUS       DAHHTG010000197         5965 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.201, whole genome shotgun sequence.
ACCESSION   DAHHTG010000197 DAHHTG010000000
VERSION     DAHHTG010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5965)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5965)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5965
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.201"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            69..1280
                     /gene="tyrP"
                     /locus_tag="NBI74_004286"
     CDS             69..1280
                     /gene="tyrP"
                     /locus_tag="NBI74_004286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine transporter TyrP"
                     /protein_id="HCD1934520.1"
                     /translation="MKNRTLGSVFIVAGTTIGAGMLAMPLAAAGVGFSVTLILLIGLW
                     ALMCYTALLLLEVYQHVPADTGLGTLAKRYLGRYGQWLTGFSMMFLMYALTAAYISGA
                     GELLASSISDWTGISMSATAGVLLFTFVAGGVVCVGTSLVDLFNRFLFSAKIIFLVVM
                     LVLLLPHIHKVNLLTLPLQQGLALSAIPVIFTSFGFHGSVPSIVSYMDGNIRKLRWVF
                     IIGSAIPLVAYIFWQLATLGSIDSTTFMGLLANHAGLNGLLQALREMVASPHVELAVH
                     LFADLALATSFLGVALGLFDYLADLFQRSNTVGGRLQTGAITFLPPLAFALFYPRGFV
                     MALGYAGVALAVLALIIPSLLTWQSRKHNPQAGYRVKGGRPALVVVFLCGIAVIGVQF
                     LIAAGLLPEVG"
     gene            complement(1331..1996)
                     /gene="yecA"
                     /locus_tag="NBI74_004287"
     CDS             complement(1331..1996)
                     /gene="yecA"
                     /locus_tag="NBI74_004287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YecA"
                     /protein_id="HCD1934521.1"
                     /translation="MKTGPLNESELEWLDDILTKYNTDHAILDVAELDGLLTAVLSSP
                     QEIEPAQWLVAVWGGADYVPRWASEKEMTRFMNLAFQHMADTAERLNEFPEQFEPLFG
                     LREVDGSELTIVEEWCFGYMRGVALSDWSTLPDSLKPALDAIALHGTEENFERVEKMS
                     PEAFEESVDAIRLAALDLHAYWMAHPQEKAVQQPIKAEEKPGRNDPCPCGSGKKFKQC
                     CLH"
     gene            complement(2192..2278)
                     /locus_tag="NBI74_004288"
     tRNA            complement(2192..2278)
                     /locus_tag="NBI74_004288"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2242..2244),aa:Leu,seq:taa)
     gene            complement(2291..2364)
                     /locus_tag="NBI74_004289"
     tRNA            complement(2291..2364)
                     /locus_tag="NBI74_004289"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2330..2332),aa:Cys,seq:gca)
     gene            complement(2418..2493)
                     /locus_tag="NBI74_004290"
     tRNA            complement(2418..2493)
                     /locus_tag="NBI74_004290"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2458..2460),aa:Gly,seq:gcc)
     gene            complement(2645..3193)
                     /gene="pgsA"
                     /locus_tag="NBI74_004291"
     CDS             complement(2645..3193)
                     /gene="pgsA"
                     /locus_tag="NBI74_004291"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HCD1934522.1"
                     /translation="MQFNIPTLLTLFRVILIPFFVLVFYLPVTWSPFAAALIFCVAAV
                     TDWFDGFLARRWNQSTRFGAFLDPVADKVLVAIAMVLVTEHYHSWWVTLPAATMIARE
                     IIISALREWMAELGKRSSVAVSWIGKVKTTAQMVALAWLLWRPNIWVEYAGIALFFVA
                     AVLTLWSMLQYLSAARADLLDQ"
     gene            complement(3250..5082)
                     /gene="uvrC"
                     /locus_tag="NBI74_004292"
     CDS             complement(3250..5082)
                     /gene="uvrC"
                     /locus_tag="NBI74_004292"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HCD1934523.1"
                     /translation="MSDQFDAKAFLKTVTSQPGVYRMYDAGGTVIYVGKAKDLKKRLS
                     SYFRSNLASRKTEALVAQIQQIDVTVTHTETEALLLEHNYIKLYQPRYNVLLRDDKSY
                     PFIFLSGDTHPRLAMHRGAKHAKGEYFGPFPNGYAVRETLALLQKIFPIRQCENSVYR
                     NRSRPCLQYQIGRCLGPCVEGLVSEEEYAQQVEYVRLFLSGKDDQVLTQLISRMETAS
                     QNLEFEEAARIRDQIQAVRRVTEKQFVSNTGDDLDVIGVAFDAGMACVHVLFIRQGKV
                     LGSRSYFPKVPGGTELSEVVETFVGQFYLQGSQMRTLPGEILLDFNLSDKTLLADSLS
                     ELAGRKINVQTKPRGDRARYLKLARTNAATALTSKLSQQSTVHQRLTALASVLKLPEV
                     KRMECFDISHTMGEQTVASCVVFDANGPLRAEYRRYNITGITPGDDYAAMNQVLRRRY
                     GKAIDDSKIPDVILIDGGKGQLAQAKNVFAELDVSWDKNHPLLLGVAKGADRKAGLET
                     LFFEPEGEGFSLPPDSPALHVIQHIRDESHDHAIGGHRKKRAKVKNTSSLETIEGVGP
                     KRRQMLLKYMGGLQGLRNASVEEIAKVPGISQGLAEKIFWSLKH"
     gene            complement(5079..5735)
                     /gene="uvrY"
                     /locus_tag="NBI74_004293"
     CDS             complement(5079..5735)
                     /gene="uvrY"
                     /locus_tag="NBI74_004293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UvrY/SirA/GacA family response regulator
                     transcription factor"
                     /protein_id="HCD1934524.1"
                     /translation="MINVLLVDDHELVRAGIRRILEDIKGIKVVGEASCGEDAVKWCR
                     ANAVDVVLMDMSMPGIGGLEATRKIARSTADVKIIMLTVHTENPLPAKVMQAGAAGYL
                     SKGAAPQEVVSAIRSVYSGQRYIASDIAQQMALSQIEPEKTESPFASLSERELQIMLM
                     ITKGQKVNEISEQLNLSPKTVNSYRYRMFSKLNIHGDVELTHLAIRHGLCNAETLSSQ
                     "
BASE COUNT         1396 a         1565 c         1452 g         1552 t
ORIGIN      
        1 aatcagccgt tatgccttaa cctgcgccgc agatatcact cataaagatc gtcaggacag
       61 aagaaagcgt gaaaaacaga actctgggaa gtgtttttat cgtggcggga accacaattg
      121 gcgcaggcat gctggcaatg ccgctggctg cggcaggtgt tggttttagc gtcacgttaa
      181 tcttgttgat tgggctttgg gcgttgatgt gctacacggc gctattactg ctggaggtgt
      241 atcagcatgt cccggcagat accggtctgg gcacgctggc aaaacgctat ctgggacgct
      301 acggtcagtg gctgacgggc ttcagtatga tgttcttaat gtatgctctg actgcggcat
      361 acatcagcgg tgccggtgaa ttgttggcct ccagcatcag cgactggaca ggtatttcaa
      421 tgtcggcaac cgctggcgtg ctgttgttca cttttgttgc cggtggcgtg gtttgtgtcg
      481 gaacttcgct ggtcgattta tttaaccgtt ttctgttcag cgccaaaatt atttttctgg
      541 tggtgatgct ggtattgctg ctaccgcata ttcacaaagt gaatctttta accctgccgt
      601 tgcaacaggg actggcactg tctgcaatcc cggtgatttt tacgtcgttt ggttttcacg
      661 gtagcgtgcc gagtattgtc agctatatgg atggcaacat tcgtaagcta cgctgggtgt
      721 ttataatcgg tagtgcgatc cccctggtgg catatatttt ctggcagctg gcgacccttg
      781 gcagcattga ttcaacaacc tttatgggat tgctggctaa tcatgccgga ttaaacgggc
      841 tgttacaggc gttacgcgaa atggtggcct ctccgcatgt tgagctggca gtgcatttat
      901 ttgctgattt agccctcgcc acgtccttcc tcggcgtagc gttaggctta tttgattatt
      961 tggcagattt gtttcagcgt tcaaataccg ttggtggacg attgcaaact ggtgcaatta
     1021 cctttctgcc gccactggcg tttgcactgt tttatccacg aggatttgtg atggcgctgg
     1081 gttacgccgg cgtggcgctg gcggtactgg cattgattat cccttcgctg ttgacctggc
     1141 aaagcaggaa gcacaatcct caggcgggtt accgggtgaa aggtggtcgt ccggcgctgg
     1201 tggtggtgtt tctctgtggt attgctgtga ttggcgtgca atttttgatt gcggcaggct
     1261 tgttaccaga agtggggtga ttagattctt attgggtgcc agaattaacg ctgacaccca
     1321 atgtgacctc ttaatgcagg cagcactgct taaatttctt accactgcca cacggacaag
     1381 gatcgttacg tcccggtttc tcttctgctt tgatcggttg ctgaacagct ttttcctgcg
     1441 gatgcgccat ccagtatgca tgtaaatcaa gcgccgccag ccgaatagca tctacgctct
     1501 cttcaaacgc ttctggcgac atcttctcta cccgctcgaa gttttcctca gtaccgtgca
     1561 gcgcaatcgc atccagcgct ggttttaacg aatcgggcaa cgttgaccag tcagaaagtg
     1621 ccacgccccg catatagcca aagcaccact cctcaacaat cgtcagctcg ctgccatcga
     1681 cttctcgcaa gccgaataac ggctcaaact gctccgggaa ttcgttcaga cgctctgcgg
     1741 tatcggccat atgttgaaaa gccaggttca taaagcgcgt catctctttc tctgacgccc
     1801 agcgcggcac atagtcagcc ccaccccaca cggcaaccag ccactgtgcc ggttcaatct
     1861 cttgcggaga actcaacacc gccgtcaata aaccgtccag ctccgccaca tcaagaatgg
     1921 cgtggtcagt gttgtatttg gtcagaatat cgtccagcca ttccaactca ctttcgttta
     1981 acggtcccgt tttcatacgc ttttccttgt ggatctcaac tcgccagcac ctatcttaca
     2041 tgccggtccg tatcagagat actttttgag tggctttgcc ggtgattaaa aattaaggag
     2101 ggtgtaacga caagttgcag gcacaaaaaa accacccgaa ggtggtttca cgacactgct
     2161 tattgctttg attttattct tatctttccc atggtacccg gagcgggact tgaacccgca
     2221 cagcgcgaac gccgagggat tttaaatccc ttgtgtctac cgattccacc atccgggctc
     2281 gggaagaaag tggaggcgcg ttccggagtc gaaccggact agacggattt gcaatccgct
     2341 acataaccgc tttgttaacg cgccaaattc ttcaggcctt tcagccagac atccgctgac
     2401 gccgatgcct tttaaactgg agcgggaaac gagactcgaa ctcgcgaccc cgaccttggc
     2461 aaggtcgtgc tctaccaact gagctattcc cgcattcatc aagcaatcag ttaatcactt
     2521 gattttatta tcgtctggca atcagtgccg ccgttcgatg cgttgcattc tacttacctg
     2581 gcgcgatgag tcaacgatat ttttcaccac ttttgatcgt ttgctgaaaa ttgcgccgaa
     2641 acgatcactg atcaagcaaa tctgcacgcg cagcgctcaa atattgcaac attgaccaca
     2701 gagtcagtac cgcagccaca aagaaaagtg caataccggc gtactcaacc caaatgttcg
     2761 gacgccacag cagccaggct aacgccacca tctgggcagt ggttttcact tttccaatcc
     2821 aggagacagc cacgctactg cgtttaccca actccgccat ccattcgcgt agcgcagaaa
     2881 taataatttc acgggcgatc atcgttgctg ccggtaaggt cacccaccag ctgtggtaat
     2941 gctcggttac cagcaccatg gcgatagcca cgagaacttt atctgcgaca gggtcaagga
     3001 aagcaccaaa ccgggtactc tggttccagc ggcgtgccag aaaaccatcg aaccagtcag
     3061 tcaccgccgc gacgcagaaa atgagcgcgg cggcaaacgg cgaccaggtg acaggcagat
     3121 aaaagaccaa tacaaagaat gggataagga tgacacggaa cagtgtaagc aacgtaggga
     3181 tattaaattg cataatgacg ggtaactatc tgttgtcagt aagattaccc ctatgttgct
     3241 acagagacat caatgtttca acgaccagaa gatcttttct gccagacctt gcgaaatacc
     3301 cggcactttt gcaatttcct cgacgctggc gttacgtaaa ccttgcaaac cgcccatata
     3361 tttcaacaac atttgcctac gttttggccc gacgccttca atggtttcca gggaactggt
     3421 atttttgacc ttcgcccgtt ttttacggtg cccaccaatc gcgtgatcgt gtgattcatc
     3481 gcgaatatgc tggataacat gcagcgcggg agaatctggc ggcaaactaa atccctcacc
     3541 ttccggctca aagaacagcg tttccagccc agccttacga tccgctcctt tggcaacgcc
     3601 aagtagcagc ggatgatttt tatcccatga gacatccagt tcggcgaaga catttttcgc
     3661 ctgcgcaagc tgtcctttgc cgccgtcgat aaggatcaca tccgggatct tactgtcgtc
     3721 aatggcttta ccataacgcc gacgcagcac ctgattcatc gccgcataat catcgcccgg
     3781 cgtgatgcca gtaatgttat agcgccgata ctccgcacgc agcgggccgt tagcatcaaa
     3841 caccacacag gaagcgacgg tttgttcacc catggtatgg ctgatgtcaa agcactccat
     3901 ccgcttcact tccggcaatt tcaacacgct ggcaagcgcg gtcagccgct ggtgaacggt
     3961 agattgctgc gaaagtttgc tggttaaggc cgtcgccgca ttggtgcgcg cgagtttcag
     4021 ataacgcgct ctatcgccac gaggtttggt ttgaacatta atcttgcgtc ccgccagttc
     4081 tgaaagggaa tcggcgagca gcgttttatc gctaagatta aaatcgagca ggatctcacc
     4141 cggtaaggtg cgcatctggc tgccttgtaa atagaactga cctacgaagg tttccaccac
     4201 ctcgctcagt tccgtaccgc caggcacttt cgggaaatag ctgcggctgc cgagcacttt
     4261 gccctgacga atgaacaata cgtggacaca agccataccc gcatcgaacg ccactccaat
     4321 aacgtcaagg tcgtcgccgg tattggaaac aaactgtttt tcggtgacac gtcgcaccgc
     4381 ctgaatttgg tcgcgaatcc gtgcagcttc ttcaaactcc agattctggc tggcagtttc
     4441 catacgacta atgagttgcg taagcacctg atcatctttg ccagacaaaa acaggcgcac
     4501 atactcgacc tgctgagcgt attcttcttc actcaccagt ccttcaacgc acggccccag
     4561 acagcgccct atctgatatt gcagacacgg acgcgagcga ttgcgataaa cactgttttc
     4621 gcactggcga atggggaaaa tcttttgcag tagcgccagt gtttcacgta cggcatagcc
     4681 attcgggaac gggccgaaat attcaccttt ggcatgcttc gctccacgat gcatcgccag
     4741 acgcgggtgg gtatcgccac tcaggaagat aaaaggatat gatttatcat cgcgtagcaa
     4801 aacgttgtaa cgcggctgat aaagtttgat gtagttgtgt tccagtaaca gcgcttcggt
     4861 ttctgtatga gtaaccgtta catcaatttg ctggatctgg gcgaccagcg cttcggtttt
     4921 gcgcgaagcg aggttgctac ggaaatagct ggaaagccgt tttttcaggt ctttcgcttt
     4981 gcctacatag ataaccgtac caccagcatc atacatgcga taaacgcctg gctggctggt
     5041 tacggttttt aaaaacgctt ttgcgtcaaa ctgatcactc actgacttga taatgtctcc
     5101 gcattacaca gaccatggcg aattgccagg tgagtcagct caacatcgcc atgaatgttt
     5161 agtttactga acatacgata gcggtagctg ttcaccgttt tcggactgag attgagctgt
     5221 tctgagatct cattgacctt ctggcccttg gtgatcatca gcataatctg caattcacgt
     5281 tcagacaaac tggcaaatgg gctttctgtt ttttctggtt cgatctggct taacgccatt
     5341 tgttgagcga tatcagaagc aatgtaacgc tgccctgaat agacagaacg aatcgcactc
     5401 acgacttcct gcggagccgc gcctttgctg aggtagcccg cagctccggc ctgcatgact
     5461 ttcgctggta aagggttttc tgtatggacg gtaagcatga tgattttgac atcagctgtg
     5521 gaacgcgcga ttttacgcgt cgcctcaaga ccgccaatgc ccggcatact catgtccatt
     5581 agcaccacgt caacggcatt tgcccggcac catttaacgg cgtcttcacc gcacgatgcc
     5641 tcaccgacga cttttatacc ctttatatct tccagaatgc gtcgtatccc tgcgcgcacc
     5701 agttcgtggt catcaacaag tagaacgttg atcaaaggaa tatctccaga aatagggata
     5761 acgctactga tagttagtca ttcgtatatt agcggttttt attgcaactt tgaaacgtta
     5821 aaaatggtgg gtattcgatt tttctctcgt ttttgtcaat tcgattgtcc acagttatgg
     5881 tcacgcccgt caccaacggc ttccgggatc gtttcacagt aagcccggcg atgaaatgtg
     5941 acaaaaattg actttattca gcaaa
//
LOCUS       DAHHTG010000198         5921 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.202, whole genome shotgun sequence.
ACCESSION   DAHHTG010000198 DAHHTG010000000
VERSION     DAHHTG010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5921)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5921)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5921
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.202"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            complement(312..1382)
                     /locus_tag="NBI74_004294"
     CDS             complement(312..1382)
                     /locus_tag="NBI74_004294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000412404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCD1934525.1"
                     /translation="MEKKLKIADIAMRTGLSSSTVSRVLAGKANTSYRAREKVLACAR
                     ELGVMDGMASGRMLLNNLVIFAPQRAFDERTDIFYFRVIQSISKALSHYEVRLRYCAL
                     DEFDSTPSKFLARMNEAETQAAILLGIDDPHIHDLAADFSKPCVMINCHDRRMRLPTV
                     APDHKNIGAFASHFLFEMGHRRVMNIMCLRRYTMELRLAGIKEAWERQNQNFKDDRDL
                     LTINSFSAKEAEEKVGAWLDMTEKSMLPTAFLASGDFIAAGIINALKKRNIRVPQDVS
                     VMSIDGFNLAAIEDVPLTAVHVPRDELGTEAVHMLQQRLVRPDATVGALLLYGKLVIR
                     ESVRRIRPGKEPTPIKGDGLYD"
     gene            1644..2945
                     /locus_tag="NBI74_004295"
     CDS             1644..2945
                     /locus_tag="NBI74_004295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001329026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCD1934526.1"
                     /translation="MSQDINNNIAVSKHRRIIRQLRWWMLILFLMGVTVNYITRNSLG
                     ILAPELKDSLGITTEQYSWIVGGFQLAYTLFQPLCGWLIDVIGLKIGFMICASLWGIA
                     CLLHAGAGSWIQLALLRFFMGGAEASATPANAKIIGEWFPKSERPVAAGWAGVGFSIG
                     AMLAPPIIYFAHASFGWQGAFMFTGALAILWVFLWWACYNTPEEHPNLSKNELNFIRQ
                     DNEAPPVKMPFLTALKTVSKNKRFYGIAIPAFMAEPAWAVMSFWVPLYLAKAYGMDLK
                     QIALFAWLPFLCADLGSIASGYLTKLYTRLFGLSRVNSVIASSVSGAFLMVSLAVIAF
                     IDNPYIAIVLISIGGFGHQIISCMLSALVVESFDKGQMATVNGMRGSAAWIASFLFSL
                     IIGVTADKIGFNPLFIAMGFFDLIGAFFLVTFIAERRKKRT"
     gene            2960..5341
                     /locus_tag="NBI74_004296"
     CDS             2960..5341
                     /locus_tag="NBI74_004296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 31 protein"
                     /protein_id="HCD1934527.1"
                     /translation="MKTLKNWKLQNQSAHHIELLVDGQHSLCLYILEENMFRVLLKRK
                     GVLSLDRTWSIAPEKDVPWEGRHREDISGFSLPTWNMEQNDELLTITTSSLRVIIHKP
                     LWLEWHYKDNAGQWQELVNDRPTSAYLINAHGDGVAHYQSRRNDERFYGLGDKSGDLQ
                     RTGKRYEMRNLDAMGYNAVSTDPLYKHIPFTITHRSDISFGLFYDNLSNSWLDLGNEI
                     DNYHTTYRRWQAEAGDIDYYLFTGRCVLDITKAFVRLTGKTLFGPKWSLGYSGSTMHY
                     TDAPDAQNQLMQFIRLCKEHAIPCDSFQLSSGYTSINGKRYVFNWNYDKVPHPKMMSQ
                     EFHNAGIHLAANIKPCLLQDHPRYNEVAEQELFIRDSEYNVPERSSFWDDEGSHLDFT
                     NPQTVAWWQEGVTTQLLEMGIDSTWNDNNEFEVWDGEARCHGFGKEIAIKHIRPVMPL
                     LMCRASMEAQQKFAPNKRPYLISRSGCAGLQRYVQTWSGDNRTNWDTLRYNTRMGLGM
                     SLSGLYNIGHDVGGFSGDKPDPELFVRWVQNGVMHPRFTIHSWNDDHTVNEPWMYPEV
                     TPAIRSAIELRYRLMPYLYTLLWQAHADDEPILRPTFLDHEHDVQTFEECDDFMVGRD
                     ILVASVVEAGQRQRRVWLPDNKTGWYDFYNGEWFCGGQWITIDAPLEKLPLLVRAGAG
                     IPLSERITYVSEAEDNHRKLKLFPIKGTGKSTGLLFEDDGETWGYTEGNALWLEWELD
                     CTATTIELRINTHGDYRPAWETLKVIIPQGESRQLLINGIEAYEWNMNLSCND"
BASE COUNT         1695 a         1195 c         1259 g         1772 t
ORIGIN      
        1 caggggttat cttctgaata tttcttttga aaaggataac acagtaaatt atttttatga
       61 attatcccat ggactcatta acaccctttc ataatgcttt attgtcaaac acgttatggc
      121 tgacatcaaa aaaaaccgga tttcctctgc cagcgggtaa tcaccttccc ggtgttttcg
      181 gttggtctgg ttactcctgt ctggttatta gcaagataat tgctataaac agtggaaaac
      241 tcatcgtaca taatctggtg atgaacatta cgcttatttt cccttgaccg gaagaatcag
      301 aggctgtggt ttcagtcata cagtccatct cctttaatcg gagttggctc tttccctggc
      361 cgtatacggc gaacagattc tcgtattacc agtttcccat acaaaagtaa tgcaccaaca
      421 gttgcatcag gacgtacaag tctttgttgt aacatatgta ccgcttcagt acccagttca
      481 tcgcgaggta catgtactgc cgtaagtggc acatcttcta ttgcagccag attaaaccca
      541 tcaatactca ttacggacac atcttgtggc actcgtatat tacgtttttt taaagcgtta
      601 attatacctg cagcaatgaa gtccccactt gccaaaaaag cagtcggtag catacttttc
      661 tctgtcatat ccagccatgc tccgactttt tcttcggctt ctttggcgct gaaactatta
      721 atagtgagta aatctctgtc atctttaaag ttctgattct gtctctccca tgcttcttta
      781 attccggcaa gtcgcagttc cattgtgtat cgacgtaaac acataatgtt cattactctg
      841 cgatgcccca tttcaaacag aaagtgagac gcaaaagcac caatgttttt gtgatctgga
      901 gcaactgtgg gaagacgcat acgtcgatca tggcagttaa tcattacgca cggcttagaa
      961 aaatctgctg caagatcatg tatatgagga tcatcaatac caagcaatat tgcagcctgt
     1021 gtttctgcct cattcatgcg cgccaggaat tttgaaggtg tactatcgaa ctcatcaagg
     1081 gcacagtaac gcaaccgaac ctcataatgc gataatgcct tgctgatgct ttggattacg
     1141 cggaaataaa aaatgtctgt tcgctcatca aaagcccgtt gaggagcaaa aatgacaagg
     1201 ttgtttaaca acatacggcc ggatgccatt ccatccataa ccccaagctc tctggcacac
     1261 gccaatactt tctcgcgggc acgataactg gtattagctt tacccgctaa aacccgtgac
     1321 acggtgctgg atgaaaggcc tgttcgcatg gcaatatcag caatctttag ttttttttcc
     1381 atattgtgat ctatctctaa tttcaaaatg aaaaaattct catgggatgg aatagcctga
     1441 ctgcaaggac ttctgactat ttttagaatt tatatgaact acttatgaaa aaacgtgcac
     1501 aataccaact ttttttcatc gcccccctct tttattgtga atgagtagtt ccggtttaag
     1561 tattaagccg aaaggtaaag tgggatgtgg caaccccgag ctgcgttgtc gctcgatcat
     1621 ttattacatc cggtggggtg attatgagtc aggatataaa taataatata gctgtcagca
     1681 aacaccgtcg aataataaga caactacggt ggtggatgtt aatccttttt ctaatgggtg
     1741 tcactgtcaa ttatattacc agaaactcat taggtatttt ggcaccagag ttaaaagaca
     1801 gccttggaat tacaactgaa caatactcct ggattgttgg tggatttcag cttgcatata
     1861 ccctttttca gccactttgt ggatggctaa ttgatgttat tgggctcaag attggattta
     1921 tgatttgtgc ttcattatgg ggaattgctt gtcttctgca tgcaggagca ggaagctgga
     1981 tacagcttgc gttgcttcgt tttttcatgg gaggtgctga ggcttctgcg acacctgcaa
     2041 atgcaaaaat tataggtgag tggtttccaa aatctgaacg tcctgttgct gctggctggg
     2101 cgggtgtcgg tttttctatc ggtgcaatgc ttgcaccacc aattatttac tttgcacatg
     2161 cgtcatttgg ttggcagggt gcatttatgt ttacaggtgc acttgcaatt ctttgggttt
     2221 tcctttggtg ggcgtgttat aacacaccag aagagcaccc aaacttaagt aaaaatgagc
     2281 tcaactttat ccgacaggat aacgaagctc ctccggtaaa aatgcctttt ctgaccgcgt
     2341 taaaaacagt tagtaaaaat aaacgctttt atggaatcgc tatccctgca tttatggcag
     2401 agccagcatg ggctgtaatg agcttttggg tgccattata tcttgcaaaa gcatatggca
     2461 tggaccttaa acaaatagct ctgtttgcat ggttaccatt tctttgcgct gatttaggaa
     2521 gtattgccag cggatatctg acaaaactct atacccgtct gtttgggtta agcagagtaa
     2581 attctgttat tgcgtcctct gtaagcggag catttctaat ggtttcgctt gctgtgattg
     2641 cttttatcga caatccatat attgctattg tacttatctc cattggtggt tttggtcatc
     2701 agataatttc ctgcatgtta agtgccctgg tcgttgaatc ttttgataag gggcagatgg
     2761 ccactgttaa tggaatgcgt ggttcagccg catggattgc cagctttttg ttctcactga
     2821 ttattggcgt tactgctgac aaaataggct ttaacccgct atttatcgcc atgggatttt
     2881 ttgatcttat tggtgctttt tttctggtta cttttattgc agaacgtcgc aaaaaacgca
     2941 cctgagggag tatgtggaga tgaaaacatt aaaaaactgg aaactacaaa atcagtcagc
     3001 ccatcatata gagctattag ttgatggtca gcattctctt tgcctgtata tactcgaaga
     3061 aaatatgttc cgggttctat taaaacggaa gggggttttg tcgctggacc gaacctggag
     3121 cattgctccg gaaaaggatg tcccgtggga agggcgtcat cgcgaagata ttagcggttt
     3181 ttcactgccg acctggaaca tggagcagaa tgatgaatta ctgacaatca cgaccagttc
     3241 attacgcgta attattcaca agcctttatg gcttgaatgg cattataagg ataatgctgg
     3301 tcagtggcag gaacttgtta atgatcgccc taccagtgct tatctgataa acgctcatgg
     3361 tgatggtgtt gcacactatc aaagtcgacg taatgacgag cgtttttatg gacttggtga
     3421 taaatccggg gatctgcaac gcacaggaaa acgctatgaa atgcggaacc tggacgcaat
     3481 gggatataat gcagtaagta cagaccctct ttataaacat attccattta caattactca
     3541 ccgtagcgat attagctttg gattatttta tgataacctc agcaatagtt ggctggattt
     3601 aggtaatgaa atagataatt atcatacaac ttaccgccgt tggcaggctg aggcgggaga
     3661 tattgattat tatcttttca ctggcagatg tgtacttgat atcaccaaag cctttgttcg
     3721 tttgacgggg aaaacactct tcggtccaaa atggagtctt gggtacagcg gttccacgat
     3781 gcactataca gatgcaccgg atgctcagaa tcaattgatg cagtttattc gtctttgtaa
     3841 ggaacatgct attccatgtg attctttcca gctatcttct ggttatactt ctattaatgg
     3901 taaacgctac gtattcaact ggaattacga caaagttcca caccctaaaa tgatgagtca
     3961 ggagtttcat aatgcaggga tacacctggc cgctaatata aaaccatgtt tattgcagga
     4021 tcatcctcgc tataacgaag tagcagaaca agagctcttt attcgtgact ctgaatacaa
     4081 cgtcccggaa cgctccagct tctgggatga tgagggttct catctggact ttacgaaccc
     4141 acaaacagtc gcatggtggc aggaaggtgt aaccacacaa ctacttgaaa tgggaattga
     4201 ttccacctgg aatgataaca atgagtttga agtatgggat ggggaagcgc gctgtcatgg
     4261 ttttggcaaa gaaatcgcca ttaaacacat tcggccagta atgcctcttt taatgtgcag
     4321 agcatctatg gaagcacagc aaaaatttgc accgaacaaa cgaccatatt tgatttcccg
     4381 ctctggatgc gccggattgc agcgttatgt tcagacatgg agtggggaca accgaactaa
     4441 ctgggatacc cttcgataca acacccgcat ggggctggga atgagcctct caggattgta
     4501 taacattggt catgatgtcg gtggtttttc tggtgataag cctgatccag aattatttgt
     4561 tcgttgggtt cagaacggtg ttatgcaccc ccgatttacg atccactcgt ggaatgatga
     4621 tcatactgtc aatgaaccat ggatgtatcc ggaagtaaca cccgcgattc gtagtgcaat
     4681 tgagttacgt tatcgtctta tgccttattt atatacatta ttgtggcagg cacatgccga
     4741 tgatgagcca atattacgtc caaccttcct tgatcatgag cacgatgttc agacatttga
     4801 agaatgtgat gattttatgg tgggacgcga tattctggtt gcaagtgtcg tggaagcagg
     4861 gcaacgacaa cgacgagtat ggttacccga caacaaaaca ggatggtacg acttttacaa
     4921 tggcgaatgg ttctgtggtg gtcaatggat aacaatcgac gcgccactgg aaaaactacc
     4981 attattagta cgtgcaggtg caggtattcc tcttagtgaa cgtataacat acgtgagtga
     5041 agcggaagat aatcatcgta aattgaaatt attcccaatt aaaggaacgg gtaaatccac
     5101 cggacttctg tttgaagatg atggcgaaac ttggggatat actgaaggta atgctctgtg
     5161 gcttgaatgg gagctggact gcacagccac tacaattgag ttaagaataa acactcatgg
     5221 agattatcgc cctgcatggg aaacattgaa agtgataatt cctcagggag aaagtcgtca
     5281 actactgatt aacggcattg aggcttatga atggaatatg aacctatcct gtaatgatta
     5341 atttattgta gagggtaaat catggccaaa taaaggcact gtaagagtaa aacttatagt
     5401 gcctttatat acaagccaga ttataacgcc atgcaatgaa acgcccccat gcatacgtag
     5461 attaactatc tcttaccatt ttacatatcc gaaagaatat gttttaacag aaaggtaaaa
     5521 catgagcgat aataaaaaag agcacttcca caatgtaata ataagatggg ttattatttt
     5581 cgccacaatt gaatacatat atttatttat aaagtttctc taaagtctta atacccatcc
     5641 catcaaaaaa caatgcgggg ttgttaaaat aacaacataa cactgttttc atgagaatag
     5701 taaagcgttt attcaaaacc gtttatagca accccataaa cccaacatag caattgagtt
     5761 taaaaaggag atttcattgt cccctatcaa ttaaacttca tgttttttag tttgtttata
     5821 aaacaacatc atatctccta taaaatactc agcagtatta aaccacctta tctttaacca
     5881 aactatttat tccgcaagcc cacacttatg gatatttacc g
//
LOCUS       DAHHTG010000199         5912 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.203, whole genome shotgun sequence.
ACCESSION   DAHHTG010000199 DAHHTG010000000
VERSION     DAHHTG010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5912)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5912)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5912
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.203"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            complement(<1..794)
                     /locus_tag="NBI74_004297"
     CDS             complement(<1..794)
                     /locus_tag="NBI74_004297"
                     /EC_number="2.7.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000629094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine kinase"
                     /protein_id="HCD1934528.1"
                     /translation="MITLAVDIGGTKISAALISDDGSFLLKKQISTPHERCPDEMTGA
                     LRLLVSEMKGTAERFAVASTGIINNGVLTALNPDNLGGLKEYPLKNIMEDITGLNGSV
                     INDAQAAAWAEYTVLPKEICDMVFITVSTGVGGGIVVNRKLLTGVSGLAGHVGHILSG
                     VTDTECGCGRRGCVEAVSSGRAIMGAAKNKLAGYSTKYIFELARQGYKEAEFLTERSA
                     STIAELIVSLKLLLDCQVVVVGGSVGLADGYVQKVSKHLSIYSEIC"
     gene            complement(828..1745)
                     /locus_tag="NBI74_004298"
     CDS             complement(828..1745)
                     /locus_tag="NBI74_004298"
                     /EC_number="4.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001149830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HCD1934529.1"
                     /translation="MQCEFKGVISALPTPYDQSQQIDMESLRKLIRFNIEQNIKGLYV
                     GGSTGEAFLQNVAEREKILETVADESDGRLTLIAHVGGISTAESEVLAKAAKKYGYHA
                     ISAVTPFYYPFSFEEHCIHYRKIIDSADGLPMVVYNIPALSGVRFSLDQINELVTIPR
                     VCALKQTSGDLFQMEQIKRNHPELVLYNGYDEIFASGLIAGADGGIGSTYNIMGWRYL
                     EIFEAVKNNDVIKAKEMQVACNQVIDTLIQSGVLAGIKTLLYYMGIINTPVCRSPFSP
                     VKEKNLDVLSKLAERLFEEHDRNKKMKII"
     gene            1897..2034
                     /locus_tag="NBI74_004299"
     CDS             1897..2034
                     /locus_tag="NBI74_004299"
                     /inference="COORDINATES: protein motif:HMM:NF024407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
                     /protein_id="HCD1934530.1"
                     /translation="MQCPVTILCVTLCDYLAPELITHSPEESDTVTLRKRRLPLEMMA
                     W"
     gene            2406..2831
                     /locus_tag="NBI74_004300"
     CDS             2406..2831
                     /locus_tag="NBI74_004300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001373091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence hypothetical
                     protein"
                     /protein_id="HCD1934531.1"
                     /translation="MEQKILSAEPRRTFSNEFKLQMVKLASQLGASVARIAREHDIND
                     NLLFKWLRLWQNEGRISRRLPVTTSSDAGVELLPVEITPDEQKEPMAALTPLLSTPSQ
                     STVSASSCKVEFRHGNMTLENPSPELLTVLIRELTGRGR"
     gene            2828..3178
                     /gene="tnpB"
                     /locus_tag="NBI74_004301"
     CDS             2828..3178
                     /gene="tnpB"
                     /locus_tag="NBI74_004301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017901265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HCD1934532.1"
                     /translation="MISLPSDTRISLVAGVTDMRKSFNGLGEQVQHVLDENPFSGHLF
                     IFRGRRSDMIKILWADADGLCLFTKRLEEGLFIWPAVRDGKVSITRSQLAMLLDKLDW
                     RQPKTSRLNALTML"
     gene            3209..4306
                     /locus_tag="NBI74_004302"
                     /pseudo
     CDS             3209..4306
                     /locus_tag="NBI74_004302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080172.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
     gene            4306..5844
                     /locus_tag="NBI74_004303"
     CDS             4306..5844
                     /locus_tag="NBI74_004303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HCD1934533.1"
                     /translation="MNDISSDDIFLLKQRLAEQEALIHALQEKLSNREREIDHLQAQL
                     DKLRRMNFGSRSEKVSRRIAQMEADLNRLQKESDTLTGRVYDPAVQRPLRQTRTRKPF
                     PESLPRDEKRLLPAAPCCPNCGGSLSYLGEDIAEQLELMRSAFRVIRTVREKHACTQC
                     DAIVQAPAPSRPIERGIAGPGLLARVLTSKYAEHTPLYRQSEIYGRQGVELSRSLLSG
                     WVDACCRQLSPLEEALHGYVLTDGKLHADDTPVPVLLPGNKKTKTGRLWTCVRDDRNA
                     GSTLAPAVWFAYSPDRKGIHPQTHLAGFSGVLQADAYAGFNELYRDGRITEAACWAHA
                     RRKIHDVHVRTPSALTEEALKRIGELYAIEAEIRGMTAEQRLAERQLKTKPLLKSLES
                     WLREKMKTLSRHSELAKAFAYALNQWPALTYYADDGWAEADNNIAENALRMVSLGRKN
                     YLFFGSDHGGERGALLYSLIGTCKLNGVEPESYLRYVLDVIADWPINRVGELLPWRVA
                     LPTE"
BASE COUNT         1523 a         1554 c         1415 g         1420 t
ORIGIN      
        1 ttacaaattt cagaatatat ggaaagatgt ttcgacactt tctgaacata cccatcagca
       61 agtccaacac ttcctccgac aacaacaacc tgacaatcaa gcagtaattt taaacttact
      121 attaattcag caatagtact ggcagaacgc tctgtcaaaa attctgcttc cttatatccc
      181 tgtctcgcta attcaaaaat atatttggtg ctatatcctg caagtttatt ttttgctgcc
      241 cccatgatgg cccgaccaga tgaaaccgct tcaacgcatc cccgacgacc acatccacat
      301 tctgtatccg ttactccgga caatatatgc ccaacatgtc cggctaatcc agaaacacct
      361 gttaataatt ttctgttaac tacaattccc cctccaaccc ccgtagaaac ggttataaaa
      421 accatatcac aaatttcttt cggcaaaact gtatattctg cccatgcggc tgcctgggca
      481 tcatttatca cacttccatt taaaccagtt atatcttcca ttatattttt cagaggatac
      541 tctttcagcc ctccaagatt gtctggattc agagctgtca atactccatt attaataatc
      601 cctgttgatg ctactgcaaa tcgctccgct gttcccttca tttcagaaac cagtagacgt
      661 aaagcccctg tcatttcgtc aggacatctt tcatgaggtg ttgatatctg ttttttcagc
      721 aaaaatgaac cgtcatcaga tatcaacgcc gctgaaattt tcgttcctcc tatatcaaca
      781 gccagtgtta tcattcattt tcacctatat aacaatcttg caattattca gattattttc
      841 atttttttgt ttctgtcatg ttcttcgaac agtcgctctg caagttttga taatacatcc
      901 agattctttt cctttaccgg agaaaaagga cttctgcata caggagtatt tataatcccc
      961 atatagtaaa gaagagtttt tatacctgcc aggacaccac tctgaataag agtgtcaata
     1021 acctggttac aggctacttg catttccttc gccttaatca catcattgtt tttgactgct
     1081 tcaaatattt caagataacg ccaccccatt atattatatg tactaccgat tcccccatct
     1141 gcacctgcaa ttaaccctga tgcaaatatt tcatcgtaac cattatataa cacaagctct
     1201 ggatgattcc ttttgatttg ttccatctgg aataaatcac cggatgtctg ttttaatgca
     1261 caaaccctgg gtattgtgac aagctcattt atctgatcta aagaaaaacg cactccgcta
     1321 agagccggta tattataaac taccatcgga agtccatctg cagaatcaat aatcttcctg
     1381 taatggatac aatgctcttc aaatgaaaaa ggataataaa atggagtaac agcagatata
     1441 gcatgatatc cgtatttttt tgcagctttt gcaagtacct cgctttctgc cgtactgatt
     1501 ccgcccacat gcgctataag tgtcagtctt ccatctgatt catcggctac tgtttcaaga
     1561 attttttctc gctctgcaac attctgaaga aatgcttctc cagtagagcc tccaacatac
     1621 aatcccttaa tattttgttc gatattaaaa cggatcagtt ttcgaagact ctccatatcg
     1681 atttgctggg attggtcata aggagtgggt aatgctgata tgaccccttt aaattcacat
     1741 tgcattttta atcaccttca cttaactgca taatgggata cctagtacac catcaagtac
     1801 aaagattatc caacagaaaa gaagcacaat gtgaagatta tcaaaaaaaa accatcagaa
     1861 agctgtcatg ccgctcagtt aaggatcggt tgatcgatcc agtgtccagt tactattctc
     1921 tgcgtaaccc tctgcgacta tcttgccccg gaactgatta cccacagccc tgaagagtcc
     1981 gacactgtca cgctacgcaa acggcgtctt cctctggaaa tgatggcctg gtgaattatc
     2041 agtatggccc atgagcgtaa ggaaccttca ccagattgtg aaccggctgg acacatgctg
     2101 ccaggtagtc gcccctttgt cgcgccgtga tccatgccca gcagggccag gaagcggggc
     2161 cgtacgccac gtgttcacgt aaacagcgca gccctgacat aacaccacgc tgtatccgct
     2221 ctggtgcaat atgaacctgc tgaccatcga tggcgtgttc taactaacac aggacacgcc
     2281 agaacacgac ataaagcttc actcgccaga tacatggtaa gcgtaaactg accgccgtat
     2341 gtagccatta agccagtatt ggtaacgtag gtgcccacct tttcatcaac tagtggacac
     2401 ctgttatgga acagaaaata ttatccgcag aaccccgcag aacattttca aatgagttta
     2461 aacttcaaat ggttaaactg gcttcacaac taggagcctc tgttgcccgt attgccaggg
     2521 aacacgatat caatgataac ctcctgttca aatggctcag gctctggcag aacgaagggc
     2581 gtatatcgcg gcgtcttccg gtaacaacct cttctgacgc tggcgttgaa ttattacctg
     2641 ttgagataac gcctgatgag cagaaagagc ctatggcggc tcttactccg cttttatcca
     2701 ctccttcaca aagcacagtc agtgccagtt cctgtaaagt tgagttccgc cacggtaaca
     2761 tgacgctgga aaatccttca ccagagctgc tcacggtgtt gatccgtgaa ctgaccggga
     2821 gggggcgatg atctcgctcc catcagacac tcgaatctcg ctggttgccg gcgttactga
     2881 tatgcgtaaa tccttcaacg gtctgggtga acaggtacaa catgtgctgg atgagaaccc
     2941 cttctccggt cacctgttca tcttccgtgg ccgacggagt gacatgatta aaatcctctg
     3001 ggctgatgct gatggtctgt gcctgttcac caaacgcctg gaggaaggcc tgtttatctg
     3061 gcctgcggtg cgtgacggta aggtatccat tacccgctcg cagctggcaa tgctcctcga
     3121 taagctggac tggcgtcagc caaaaacatc ccgtcttaac gcactgacaa tgttgtaaaa
     3181 atgtcatggc cggattataa aaacggtcat gagtcggaaa tacctcattc gtatcactga
     3241 gctggaaagg ctgctctctg agcaggctga agccctccgt cagagagatc tgcaactgag
     3301 tctggttgaa gagacggagg ccttcctgcg ctctgcactg gcacgtgccg aagaaaagat
     3361 cgaagaggag gagcgggaaa tagaatatct gagggcccag atagaaaaac tgcgccggat
     3421 gctgttcggt acccgttctg aaaaactgca gcgtgaggtt gaacaggctg aggcccaact
     3481 gaaacaacgc gaacaggaaa gcgatcgtta cagtgggcgt gaggatgacc cgcaggttcc
     3541 ccgccagttg cgacagtcac gtcatcgtcg tccgttacca gcacatcttc cccgtgaaat
     3601 acaccgcctg gagcctgaag aaagttgttg cccggagtgt ggcagtgagc tggattatct
     3661 gggtgaagtc agtgctgagc agctggaact ggtgagtagc gccctgaaag tgatccgcac
     3721 tgttcgggta aaaaaagcct gtacaaaatg tgactgtata gttgaagcgc cagcgccgtc
     3781 ccgcccgata gcgcgcggca tcgcgggctc cggattactt gcccgcgtgt taacgggaaa
     3841 atactgcgaa cacctgccac tgtatcgtca gagtgaaatc tttgcccgac agggtgccga
     3901 actgagccgt gcattaatct ccaactgggt tgacgcgtgc tgccagttaa tgactccgct
     3961 aaatgatgcc ctgtaccgtt acgtgatgaa cacccgcaag gttcacactg acgacacacc
     4021 ggtaaaagtg ctgacaccgg gcagaaaaaa ggcaaaaaca ggacgcatct ggacgtatgt
     4081 ccgggatgac cggaatgcgg gctcatcaga gccaccggcg gtctggttcg cctactcacc
     4141 agacaggcag ggaaaacatc cggtacaaca ccttcgtccc ttccggggta tcctgcaggc
     4201 ggatgcattc agcggttacg atcggctgtt cagtgccaaa cgtgaaggtg gtgcacagac
     4261 agaagttgcg tgctgggtcc atgcccggcg aaaaatacac gatgtatgaa cgacatctct
     4321 tctgacgaca ttttcctgct gaaacagcgc ctggccgaac aggaagcgct gatccacgcc
     4381 ctgcaggaaa agctgagcaa ccgggagcgc gaaatagacc atctgcaggc gcagctggat
     4441 aaactccgcc ggatgaactt cggcagtcgt tccgaaaaag tctcccgccg tatcgcacaa
     4501 atggaagccg atctgaaccg gcttcagaaa gagagcgata cgctgactgg tagggtgtat
     4561 gacccggcag tacagcgtcc gttgcgtcag acccgcaccc gtaagccgtt ccctgaatca
     4621 ctaccccgtg acgaaaagcg actgttgcct gcggcgccgt gctgcccgaa ctgcggcggt
     4681 tcactgagct atctgggcga ggatatcgcc gaacagctgg agttgatgcg tagcgccttc
     4741 cgggttatcc ggacggtacg ggaaaaacat gcctgtactc agtgcgatgc catcgtgcag
     4801 gcacctgcac cttcgcggcc catcgagcgg ggtatcgccg gaccggggct gctggcccgc
     4861 gtgctgacct cgaagtatgc agagcacacc ccgctgtatc gccagtcaga aatatacggc
     4921 cggcaaggtg tggagctgag ccgttcactg ctgtcgggct gggtggatgc atgctgccgg
     4981 cagctgtctc cgctggaaga ggcgcttcat ggctatgtgc tgactgacgg taagctccat
     5041 gctgatgaca cgcctgtccc ggtgctgttg ccaggcaata agaaaacgaa gaccgggcgg
     5101 ttatggacct gcgttcgtga cgaccgtaac gccgggtcaa cgctggcgcc ggcggtgtgg
     5161 ttcgcttaca gcccggacag aaaaggcatc catccgcaga cccatcttgc ggggttcagt
     5221 ggtgtactgc aggcggatgc atacgccggg ttcaacgagc tgtaccggga tggccggata
     5281 acggaagccg cctgttgggc tcacgcccgc cgtaaaatcc acgatgtgca cgttcgcacc
     5341 ccgtcagccc tgacggagga agcgctgaaa cggatcggcg aactgtacgc catcgaggca
     5401 gagataaggg gaatgacggc ggagcagcgc cttgccgaac gtcagttgaa aacgaaaccg
     5461 ctgctgaaat ccctggaaag ctggctgcgt gaaaagatga aaaccctgtc gcgacactca
     5521 gaactggcga aagcgttcgc atacgccctg aaccagtggc cggcgctgac gtactatgca
     5581 gatgatggct gggctgaggc ggacaataac atcgctgaaa atgcgttgcg gatggtcagt
     5641 ctgggccgca aaaactacct gttcttcggt tcggatcatg gaggagagcg gggagcgctg
     5701 ctgtacagcc tgatcgggac gtgcaaactg aacggagtgg agccagaaag ctacctccgc
     5761 tatgtccttg acgtcatagc cgactggccg ataaaccggg tcggcgaact gctcccctgg
     5821 cgcgtagcac tgccgactga ataacacatc cccgtcaata cggtttttgc tgcacgctta
     5881 cccttctgtt gctcactcag ggcatgtaaa tc
//
LOCUS       DAHHTG010000200         5827 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.204, whole genome shotgun sequence.
ACCESSION   DAHHTG010000200 DAHHTG010000000
VERSION     DAHHTG010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5827)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5827)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5827
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.204"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            <1..348
                     /gene="smrB"
                     /locus_tag="NBI74_004304"
     CDS             <1..348
                     /gene="smrB"
                     /locus_tag="NBI74_004304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease SmrB"
                     /protein_id="HCD1934534.1"
                     /translation="EGPVKYVRPDVSHFEAKKLRRGDYSPELFLDLHGLTQLQAKQEL
                     GALIAACRREHVFCACVMHGHGKHILKQQTPLWLAQHPHVMAFHQAPKEYGGDAALLV
                     LIEVEEWLPPELP"
     gene            complement(408..1232)
                     /locus_tag="NBI74_004305"
     CDS             complement(408..1232)
                     /locus_tag="NBI74_004305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001527383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2544 domain-containing protein"
                     /protein_id="HCD1934535.1"
                     /translation="MKKMSGLFFLLLTGLLSATAWADVTFRDVKTYTGFWGNGATTKV
                     VFRIDVLTPEGVYYGEYAQDGYTLWLGNIPNTVWTGPGSAPTMNITHQSNTVDNSNCP
                     GITDGWSCVDLGVDIIVHGETHGCPWLVSTNTVSTAARGYTYIGPSAKQTRCPTEPVG
                     PYDISWNENYVLHNKMLTLQSTGGVITQTLSTYLMRDGRVCDGSRMDDRGAYCRFMSY
                     MITFSTSGCDDSKVTVTPVAHPITDKQLHDMQIRIDTSSQQPIDATCRFQYILNEL"
     gene            complement(1234..1761)
                     /locus_tag="NBI74_004306"
     CDS             complement(1234..1761)
                     /locus_tag="NBI74_004306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001050113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCD1934536.1"
                     /translation="MSLKRIFHCAAALAAVLLTPNVQAADNLHFYGNLLSKSCTLVVQ
                     GEVLAEVHFPTVSRRDLMVTGQSARVPVVFQLKDCKGPAQYEVRVTLTGTEDSEQPGF
                     LALDAASTAQGVGIGMEKTDGTAVLINNTSGATFVLSSGNNDLHFNAWLQAKSGRDVT
                     MGDFTASLTATFEYI"
     gene            complement(1758..2237)
                     /locus_tag="NBI74_004307"
     CDS             complement(1758..2237)
                     /locus_tag="NBI74_004307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001607804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCD1934537.1"
                     /translation="MKRFALCLFGCYCGIAQAADSDITFHGTLVSPPACAISDGKTVE
                     VEFRDVIIDNINGNNFRQNVPYTITCDPDVRDDAWEMTLSWTGSQTSYDNAAIETNVT
                     GLGIELQQNGQPFTLGTPLKINPSTPPTLQAVPVKASDASLSEGTFSAWATLQVDYQ"
     gene            complement(2234..2725)
                     /locus_tag="NBI74_004308"
     CDS             complement(2234..2725)
                     /locus_tag="NBI74_004308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000679363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCD1934538.1"
                     /translation="MKGVLLPVAGMVLLACGSAWADSKTVNLTLRILVDAPPPCTVKG
                     SAVEFGNVIIKNIDGSNYRQPVGYTLNCSNSVSDDLQMQLQGTTTTINGETVLSTGIN
                     GFGIRIQDASSHAPVALAGNNWLPFNVNSRPGFEAVPVKQSGTQLTASEFSATMTMVV
                     EYQ"
     gene            complement(2742..3494)
                     /locus_tag="NBI74_004309"
     CDS             complement(2742..3494)
                     /locus_tag="NBI74_004309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000120669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HCD1934539.1"
                     /translation="MFYKLSCSMVVSTTLAVLLTTTNLAAHASVTPDRTRLVFNESDK
                     SISVTLRNNNEKLPYLAQSWLEDEKGNKITSPLAVLPPVQRIDAMMNGQVKIQALPDI
                     HTLPSDRESLFYYNVREIPPKSGKANTLQIALQTRIKLFWRPKALEKIDMRKPWQFKV
                     TLTRSGQDYIVNNPTPYHVIISDASTQKKGLTAAGFKPLVMPPKTSQPLKAKMNSAPV
                     LTYINDYGARLPLVFRCEGDTCKVDEAQSLKG"
     gene            complement(3514..>5827)
                     /locus_tag="NBI74_004310"
     CDS             complement(3514..>5827)
                     /locus_tag="NBI74_004310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="outer membrane usher protein"
                     /protein_id="HCD1934540.1"
                     /translation="KSLQWTHNDECLKPGQLDGMEVENDLSQSALLLTVPQAYLEYTS
                     SDWDPPSRWDDGISGLIADYSLNAQTRHQEQGGEDSHDISGNGTVGANLGAWRFRADW
                     QSDYQHTRSNDDEDDSSNSTTSKNWDWSRYYAWRALPSLKAKLSLGEDYLNSDIFDGF
                     NYIGSSVSTDDQMLPPNLRGYAPDVSGVAHSSAKVTISQMGRVLYETQVPAGPFRIQD
                     IGDSVSGTLHVRVEEQNGQVQEYDVTTASMPFLTRQGQVRYKVMMGRPEDWNHKTEGG
                     FFSGGEASWGVADGWSLYGGALADEHYQSAAMGVGRDLAQFGALAFDVTHSHVNLDHD
                     SAYGKGKLDGNSFRVSYAKDFDELNSRVTFAGYRFSEKNFMTMSEYLDASQSDMARTG
                     NDKEMYTITYNQNFAAAGVSVYLNYSHRTYWDRPEQTNYNLMFSHYFNMGSIRNMSIS
                     VTGYRYEYDDNTDKGMYLSMSIPWSDSSTVTYNGSYGSGSDSSQVGYFNRIDDATHYQ
                     INVGTSEQHGSVDGYLSHDGTLAKVDLSANYHEGEYRSAGIALQGGATLTAHGGALHR
                     TQNMGGTRLLIDADGIANVPVESNGAPVYTNMFGKAVVADINNYYRNQAYIDLNNLPE
                     DAEATQSVVQATLTEGAIGYRKFKVISGQKAMAVLRLRDGSYPPFGAEVKNDEQQQVG
                     IVDDEGNVYLAGINAGEHMMVFWEGSAQCEIVLPKPLPADLFRGLLLPCEQKGTTAPD
                     SLAPEIKPVIQDQTRQVTPSEAPTSISATQ"
BASE COUNT         1237 a         1625 c         1497 g         1468 t
ORIGIN      
        1 gaaggtccgg tgaaatatgt tcgcccggat gtcagccatt ttgaggcgaa gaagttacgc
       61 cgtggcgatt attcaccgga gttgtttctt gatttgcacg gtctgacgca gctacaagcc
      121 aaacaggaac tgggggcgtt gattgccgcc tgccgccgtg aacatgtgtt ttgtgcctgc
      181 gtgatgcatg ggcacgggaa gcatattttg aagcagcaaa cgccgctgtg gctggcgcaa
      241 catccacatg tgatggcatt tcaccaggca ccaaaagagt atggtggtga tgcggctttg
      301 ttggtgttga ttgaagtgga agagtggctg ccgccggaat tgccctaata aatattgcgg
      361 tataaacgtc ttctccgacc aggatactga ccggagaaga attaacccta caactcattc
      421 aaaatgtact ggaaacgaca ggtggcatcg attggctgct gactgctggt gtcgatccgt
      481 atttgcatat catgcagttg cttatcggta attggatgtg ctaccggcgt tactgtgact
      541 ttgctgtcgt cacaccccga cgtgctgaat gtgatcatat acgacataaa gcgacaataa
      601 gcgccacgat cgtccattcg gcttccatca cacacccgcc cgtcgcgcat caggtaagtc
      661 gagagcgttt gtgttatgac tccccccgtc gattgcagcg ttagcatctt gttatggagg
      721 acgtagttct cattccagga gatatcatag gggccaaccg gctccgttgg gcaccgggtt
      781 tgttttgctg atggaccaat gtaagtataa cctctcgcag cagtactcac cgtgttggta
      841 ctgaccaacc acgggcaccc gtgagtttca ccatgcacga tgatatcgac ccccagatca
      901 acacatgacc atccatccgt aatccctggg caattggagt tatccacagt attactctga
      961 tgagtgatgt tcatcgttgg tgctgatccc ggtcccgtcc aaaccgtgtt agggatattc
     1021 cccaaccaca acgtatatcc atcctgtgca tattcgccat aatagacccc ttccggcgtc
     1081 aagacatcga tacgaaagac aacttttgtt gtagccccgt taccccaaaa tccggtataa
     1141 gtcttcacat ccctgaatgt gacgtccgcc caggcggttg cactcagtag cccggtcagc
     1201 agtaaaaaaa acagtccgct catttttttc atgttaaata tactcaaacg tcgcagtgag
     1261 ggaggccgta aagtcaccca tcgtcacatc ccgcccgctt tttgcctgta gccaggcatt
     1321 aaaatgcaga tcattgttgc cgctgctcag cacaaatgtc gccccgctgg tattgttgat
     1381 tagcacagca gtgccgtcgg tcttttccat gccaatcccc acaccttgtg ctgtcgaagc
     1441 ggcatccagc gccagaaaac cgggctgctc gctgtcttcc gttccggtaa gtgtcacccg
     1501 aacctcatac tgagcaggcc ctttgcaatc tttaagctga aacaccaccg gtacgcgcgc
     1561 agattgcccg gtcaccatca gatctctacg gctgaccgtg gggaaatgca cttccgccag
     1621 cacctcgccc tgaaccacca gcgtgcagga cttactgagt aaattgccgt agaaatgcag
     1681 gttgtctgcc gcctgaacat tcggcgtcag cagcaccgcg gccagcgcag ccgcgcagtg
     1741 aaatatccgt tttaggctca ttggtaatcc acctgtaatg ttgcccaggc agaaaaggtt
     1801 ccttcgctga gtgaagcatc actggctttc accggtacag cttgcagcgt tggcggcgtt
     1861 gacgggttga ttttcagcgg cgtacccaac gtgaacggct ggccattttg ttgcagctca
     1921 atccccagcc cggtgacgtt tgtctcaata gccgcgttat cgtagctggt ctggctgccc
     1981 gtccagctca gcgtcatctc ccaggcatcg tcgcgcacgt cgggatcgca tgtaatggta
     2041 tagggcacat tctggcggaa gttattgccg ttgatgttgt cgataatgac gtcacgaaac
     2101 tcaacttcaa cggttttccc gtcactgatg gcacaggcgg gcggcgaaac caatgtgcca
     2161 tgaaatgtga tatcgctatc ggccgcctgt gcaataccgc aataacaacc gaataagcag
     2221 agcgcaaatc gtttcattga tactccacca ccattgtcat ggttgcgcta aactctgatg
     2281 ccgtaagctg tgtgccgctt tgtttcaccg gcacggcttc gaaacccggg cgcgaattaa
     2341 cgttaaacgg cagccagtta ttacccgcca acgcaaccgg cgcatggctg gacgcatcct
     2401 gtatgcgaat gccaaaacca ttgatgcccg tactgaggac cgtttcaccg ttaatggtgg
     2461 tggtggtccc ctgtaactgc atctgtaggt catcgctgac gctgttcgaa cagttcaggg
     2521 tgtaaccaac aggctgccga tagttgctgc cgtcgatgtt tttaataatg acattgccaa
     2581 actccaccgc gctgcctttt accgtacagg gcggcggtgc atccaccagt atacgaagcg
     2641 ttaagttgac cgttttacta tcggcccagg cggagccgca ggccagtaac accatgccag
     2701 cgacgggtaa taaaactcct ttcatgctgg atctcctgtc attagccttt taggctttga
     2761 gcttcatcca ccttgcaggt gtctccttca caacggaaga ccagcggtaa acgtgcgccg
     2821 tagtcgttga tgtaggtcag caccggagcg ctgttcattt ttgcctttaa cggttgtgac
     2881 gttttcggcg gcatcaccag tggtttaaag cccgcagcag ttagcccctt tttctgcgta
     2941 ctggcatcgc tgatgatgac gtggtacggc gtcgggttat tgacgatata gtcctggccg
     3001 ctgcgcgtga gtgtgacctt aaactgccac ggttttctca tgtcaatctt ctccagcgcc
     3061 tttggtcgcc agaacagctt gatgcgggtt tgcagcgcaa tttgcagggt gttggctttc
     3121 ccggactttg gcgggatctc acgcacattg tagtagaaca ggctttcacg gtcagacggc
     3181 agcgtgtgaa tgtccggcag cgcctgaatc ttcacctgac cgttcatcat ggcgtcaata
     3241 cgctgcaccg gcggcagtac cgccagcggc gaagtgattt tgttgccctt ctcgtcttcc
     3301 agccagcttt gcgccagata aggcagcttt tcgttattgt tgcgcagggt tacgcttatc
     3361 gatttatcac tttcgttaaa caccagacgg gtgcgatccg gagtgactga tgcgtgcgcc
     3421 gccaggttgg tggtggtcaa cagtaccgcc agcgtcgtgc tgaccaccat tgaacaggat
     3481 aatttataaa acattgattg ttccttaatc atattattga gtggctgaaa ttgacgtcgg
     3541 cgcttcagat ggcgtgacct gccgagtctg gtcctgaata acgggcttaa tttccggcgc
     3601 taaagaatcg ggggctgtcg ttcccttttg ttcacacggc aacaacaggc cgcggaacag
     3661 atcggcaggt agcggcttcg gcaatacgat ctcgcattgt gcgctgcctt cccagaacac
     3721 catcatatgc tcacccgcat tgatccccgc cagatagaca ttgccttcat catccacaat
     3781 gccaacctgc tgctgctcgt cgttttttac ttccgcgcca aacggcgggt agctaccgtc
     3841 acgcaaccgc agtaccgcca tcgctttttg cccgctgatc actttgaatt tacgatagcc
     3901 gattgcgcct tccgtcagag tcgcctgcac caccgactgg gtggcttccg catcttccgg
     3961 caagttgttt aaatctatat acgcctgatt gcgatagtag ttgttaatat cggcgaccac
     4021 cgccttgcca aacatattgg tgtacaccgg cgcgccgttg ctttcgaccg gcacgttggc
     4081 aataccgtcg gcgtcaatca gcaggcgtgt gccgcccatg ttttgagtgc gatgcagcgc
     4141 cccaccgtgc gcggtcagcg ttgccccgcc ctgtaaggcg atccccgccg aacggtattc
     4201 tccttcatgg tagttggcgc tgagatctac cttcgccaac gtgccgtcgt ggctcagata
     4261 accatccacg ctaccgtgct gttcgctggt gccgacgtta atctggtagt gcgttgcgtc
     4321 atcgatgcga ttaaagtagc cgacctgact gctgtccgac ccgctaccgt aagagccgtt
     4381 gtaggtcacg gtgctgctgt cgctccacgg aatgctcatg ctgaggtaca ttcccttatc
     4441 cgtattatcg tcatattcgt agcgatagcc ggtcaccgag atgctcatat tgcgaatgct
     4501 ccccatatta aaatagtggg aaaacatcag gttatagttt gtctgttccg ggcgatccca
     4561 gtaagtacga tgggagtagt tgagatagac cgagacaccc gctgcggcaa agttctggtt
     4621 atacgtgatc gtatacatct ctttgtcgtt tccggtccgc gccatgtccg actggctcgc
     4681 gtccagatac tcgctcatag tcataaagtt cttttcggag aaacggtagc ctgcaaaggt
     4741 gacgcggctg ttaagttcgt caaagtcttt ggcatagctc acgcgaaagg agttgccgtc
     4801 cagttttcct ttgccgtatg cgctgtcatg atccaggttg acgtgcgagt gagtcacatc
     4861 gaacgccagc gcgccaaact gtgcgaggtc gcgccccacc cccatcgccg ccgactggta
     4921 gtgctcatct gccagcgcgc caccgtaaag cgaccagcca tctgccaccc cccatgatgc
     4981 ttctccgccg gaaaaaaatc cgccttcggt cttgtggttc cagtcttccg gacgccccat
     5041 catcactttg taacgcacct gcccctgacg cgtgaggaat ggcatggatg cggtggtgac
     5101 gtcatattcc tgcacctgac cattctgttc ttcaacacgg acgtgtagtg tgccagagac
     5161 agaatcgccg atatcctgaa tgcggaatgg cccggcggga acctgggttt cgtaaagtac
     5221 ccggcccatc tggctgatgg tcacttttgc gctgctgtgc gccacgccag aaacatctgg
     5281 cgcatagccg cgcagattgg gcggcagcat ctggtcatcg gtgctgacac tgctgccgat
     5341 atagttaaag ccgtcgaaaa tatcggaatt gagataatct tctcccagcg acagcttcgc
     5401 ttttaaagag ggtaaagctc gccaggcgta ataacggctc cagtcccagt ttttactcgt
     5461 tgtactgtta ctgctgtcat cttcatcgtc gttgctgcgg gtgtgctgat aatcactttg
     5521 ccagtctgca cggaaacgcc atgccccaag gtttgcccca actgtaccgt taccgctgat
     5581 atcatgtgag tcctcgcccc cttgctcctg atggcgagtt tgcgcattga ggctgtagtc
     5641 ggcaatcagc ccagaaatac catcgtccca gcgtgaggga gggtcccagt cgctgctggt
     5701 atattcgagg taagcctgtg gcactgtcag cagcaaagcc gactggctta aatcattctc
     5761 gacttccatc ccgtctaatt gccccggttt aaggcattca tcgttgtgcg tccactgcag
     5821 gcttttt
//
LOCUS       DAHHTG010000201         5779 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.205, whole genome shotgun sequence.
ACCESSION   DAHHTG010000201 DAHHTG010000000
VERSION     DAHHTG010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5779)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5779)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5779
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.205"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            complement(347..1306)
                     /gene="nrdF"
                     /locus_tag="NBI74_004311"
     CDS             complement(347..1306)
                     /gene="nrdF"
                     /locus_tag="NBI74_004311"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HCD1934541.1"
                     /translation="MKLSRISAINWNKISDDKDLEVWNRLTSNFWLPEKVPLSNDIPA
                     WQTLSVVEQQLTMRVFTGLTLLDTLQNVIGAPSLMPDALTPHEEAVLSNISFMEAVHA
                     RSYSSIFSTLCQTKDVDAAYAWSEENAPLQRKAQIIQQHYRGDDPLKKKIASVFLESF
                     LFYSGFWLPMYFSSRGKLTNTADLIRLIIRDEAVHGYYIGYKYQKNLEKISQARREEL
                     KSFAFDLLLELYDNELQYTDELYAETPWADDVKAFLCYNANKALMNLGYEPLFPAEMA
                     EVNPAILAALSPNADENHDFFSGSGSSYVMGKAVETEDEDWNF"
     gene            complement(1316..3460)
                     /gene="nrdE"
                     /locus_tag="NBI74_004312"
     CDS             complement(1316..3460)
                     /gene="nrdE"
                     /locus_tag="NBI74_004312"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HCD1934542.1"
                     /translation="MATTTAERLTQETMDYHALNAMLNLYDSAGRIQFDKDRQAVDAF
                     MTTHVRPNSVAFSSQQQRLNWLVNEGYYDESVLNRYSRDFVITLFAHAHASGFRFQTF
                     LGAWKFYTSYTLKTFDGKRYLEDFADRVTMVALTLAQGDETLATQLTDEMLSGRFQPA
                     TPTFLNCGKQQRGELVSCFLLRIEDNMESIGRAVNSALQLSKRGGGVAFLLSNLREAG
                     APIKRIENQSSGVIPVMKMLEDAFSYANQLGARQGAGAVYLHAHHPDILRFLDTKREN
                     ADEKIRIKTLSLGVVIPDITFHLAKENAQMALFSPYDVERVYGKPFADVAISERYDEL
                     VADERIRKKYLNARDFFQRLAEIQFESGYPYIMYEDTVNRANPIAGRINMSNLCSEIL
                     QVNSASEYDENLDYARTGHDISCNLGSLNIAHTMDSPDFARTVETAVRGLTAVSDMSH
                     IRSVPSIEAGNAASHAIGLGQMNLHGYLAREGIAYGSPEALDFTNLYFYTITWHALRT
                     SMLLARERGETFAGFKQSRYASGEYFSQYLQSNWQPKTAKVGELFARSGITLPTREMW
                     AQLRDDVMRYGIYNQNLQAVPPTGSISYINHATSSIHPIVAKVEIRKEGKTGRVYYPA
                     PFMTNENLALYQDAYEIGAEKIIDTYAEATRHVDQGLSLTLFFPDTATTRDINKAQIY
                     AWRKGIKTLYYIRLRQMALEGTEIEGCVSCAL"
     gene            complement(3433..3843)
                     /gene="nrdI"
                     /locus_tag="NBI74_004313"
     CDS             complement(3433..3843)
                     /gene="nrdI"
                     /locus_tag="NBI74_004313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HCD1934543.1"
                     /translation="MSQLVYFSSSSENTQRFIERLGLPAVRIPLNERERIQVDEPYIL
                     IVPSYGGGGTAGAVPRQVIRFLNDEHNRALLRGVIASGNRNFGEAYGRAGDVIARKCG
                     VPWLYRFELMGTQSDIENVRKGVTEFWQRQPQNA"
     gene            complement(3840..4085)
                     /gene="nrdH"
                     /locus_tag="NBI74_004314"
     CDS             complement(3840..4085)
                     /gene="nrdH"
                     /locus_tag="NBI74_004314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HCD1934544.1"
                     /translation="MRITIYTRNDCVQCHATKRAMENRGFDFEMINVDRVPEAAEALR
                     AQGFRQLPVVIAGDLSWSGFRPDMINRLHPAPHAASA"
     gene            complement(4332..4661)
                     /locus_tag="NBI74_004315"
     CDS             complement(4332..4661)
                     /locus_tag="NBI74_004315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 domain-containing protein"
                     /protein_id="HCD1934545.1"
                     /translation="MFNRPNRNDVDDGVQDIQNDVNQLADSLESVLKSWGSDAKGEAE
                     AARSKAQALLKETRARMHGRTRVQQAARDAVGCADSFVRERPWCSVGTAAAVGIFIGA
                     LLSMRKS"
     gene            4813..5157
                     /locus_tag="NBI74_004316"
     CDS             4813..5157
                     /locus_tag="NBI74_004316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgaC family protein"
                     /protein_id="HCD1934546.1"
                     /translation="MYLRPDEVARVLEKVGFTVDVVTQKAYGYRRGENYVYVNREARM
                     GRTALVIHPTLKERSSTLAEPASDIKTCDHYQQFPLYLAGERHEHYGIPHGFSSRVAL
                     ERYLNGLFGEAS"
     gene            complement(5194..5643)
                     /gene="alaE"
                     /locus_tag="NBI74_004317"
     CDS             complement(5194..5643)
                     /gene="alaE"
                     /locus_tag="NBI74_004317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-alanine exporter AlaE"
                     /protein_id="HCD1934547.1"
                     /translation="MFSPQSRLRHAVADTFAMVVYCSVVNMCIEVFLSGMSFEQSFYS
                     RLVAIPVNILIAWPYGMYRDLFMRAARKVSPSGWIKNLADILAYVTFQSPVYVAILLV
                     VGADWHQIMAAVSSNIVVSMLMGAVYGYFLDYCRRLFKVSRYQQVKA"
BASE COUNT         1461 a         1426 c         1441 g         1451 t
ORIGIN      
        1 agattcctgt tatttgttta tgtcgataca ttacccaatg gaaatagtca cttttttcta
       61 ccctaacata ctgagaatct gaggcaaccc ctgatggcaa atgtggcatg aatattgatg
      121 tgagcaaatc gccggaattt cccatgatat aagggctgag agcaaatcga gaaaagttga
      181 tattttttgt caaagatagc aaaaaaacgc ctgaatattg tggtttcagg cgaataatgc
      241 aacagagtat cagtgtagat caccacaaat tatttgcgtg atataaagct atttggcggg
      301 aattatttcc ctgcagcggg tagtgatatt tttgaaaata accccctcag aaattccagt
      361 cttcatcttc tgtttcgacc gcttttccca tcacatatga agagcctgaa ccggaaaaga
      421 aatcgtgatt ttcatcggca ttcggcgaaa gcgcggcgag gattgccgga ttcacttccg
      481 ccatttctgc gggaaatagc ggttcgtagc ccagattcat caacgcctta ttggcgttgt
      541 aacagagaaa cgctttcaca tcgtcagccc acggggtttc ggcgtacagc tcatcggtgt
      601 attgcaactc gttgtcatag agttccagca acaaatcgaa ggcgaaactc ttcaattctt
      661 cacgtcgtgc ctgggagatc ttttccaggt ttttctgata tttatagccg atgtagtaac
      721 cgtggactgc ttcatcccgg ataatcagac ggatcaggtc tgcggtattg gttagctttc
      781 cgcggctgga aaaatacatc ggcagccaga aaccggaata gaacaaaaaa gattcaagaa
      841 acacactggc gattttcttt ttcagcggat catcaccgcg ataatgttgc tgaataatct
      901 gtgcttttcg ctgcaacggc gcgttttctt cactccaggc gtaggcggca tcgacatctt
      961 tggtctggca cagcgtcgag aaaattgaac tgtaagagcg ggcatgaacc gcttccataa
     1021 agctgatatt tgataatacc gcctcttcat ggggggtgag tgcatcgggc atcagcgagg
     1081 gcgcgccgat aacattttgc agcgtgtcga gcagcgtcag gccagtaaaa acgcgcattg
     1141 ttagttgttg ttctacgaca cttaatgtct gccatgcagg aatatcgttc gacagtggca
     1201 ccttttctgg tagccagaaa ttgctggtca ggcgattcca cacctccaga tctttatcgt
     1261 cagatatctt gttccagttg atggcgctga tacgtgagag tttcatagat attccttaaa
     1321 gtgcacagga gacgcagcct tcaatttcag tgccttccag cgccatctga cgcaggcgaa
     1381 tgtaatagag cgttttgata cccttgcgcc aggcgtaaat ctgcgctttg ttgatatcgc
     1441 gagtggtggc ggtatcgggg aaaaacagcg tcagcgacag cccctgatcg acatggcgag
     1501 tcgcttccgc gtaggtgtcg atgatctttt ctgcgccgat ttcgtaagcg tcctgataca
     1561 gcgccagatt ttcgttagtc ataaacgggg cagggtagta aacgcgcccg gttttgccct
     1621 ctttgcgtat ctctactttc gccacaatcg gatgaatact cgacgtggca tggttgatat
     1681 aagagataga accggttggc ggcaccgcct ggagattctg gttgtagata ccatagcgca
     1741 tcacgtcgtc gcgcagctgc gcccacatct cacgggtagg taacgtaata ccgctacggg
     1801 caaacagttc gccaactttc gccgttttcg gctgccagtt actttgcaga tattggctga
     1861 aatattcacc actggcatag cgtgactgtt tgaacccggc gaaggtttca ccgcgttcgc
     1921 gcgccagcaa catcgaggta cgcagtgcgt gccaagtgat ggtatagaaa tagagattgg
     1981 tgaaatcgag tgcttccggc gaaccataag cgatgccttc tcgcgccaga tagccgtgta
     2041 aattcatctg ccccagtccg atggcgtgcg aggcggcatt tccggcttcg atggacggca
     2101 cgctgcggat atgactcata tctgacaccg ccgttaaacc gcgtacggca gtctctaccg
     2161 tgcgggcaaa gtcgggggaa tccatggtgt gggcaatatt caacgaccct agattgcagg
     2221 aaatatcatg gcctgtacgg gcatagtcga gattctcgtc atattctgag gcgctgttaa
     2281 cctgcaaaat ttctgagcag agattgctca tatttatgcg cccggcgata gggttagcac
     2341 ggttaaccgt gtcttcatac atgatgtacg gatagccaga ctcaaactgg atctctgcca
     2401 gtcgctggaa gaaatcacga gcgttgaggt attttttgcg aatgcgttca tcggcaacca
     2461 gttcgtcata gcgttcgctg atggcgacat cggcaaacgg tttgccataa acccgctcaa
     2521 cgtcataggg cgaaaacagc gccatctgcg cattctcttt tgccagatgg aaagtgatat
     2581 ccgggatcac cacgcccagc gacagtgttt taatgcggat tttttcgtcg gcattttccc
     2641 gttttgtatc gagaaaacgc agaatatcgg gatgatgagc atgtaaatag actgccccag
     2701 ccccctgacg cgcgccgagt tggttggcat aggaaaatgc gtcttccagc attttcatca
     2761 ccggaatcac gccagaagat tgattttcaa tacgtttaat tggcgcgccc gcttcccgca
     2821 gattcgacag caaaaatgct acgccgccgc cgcgtttcga cagttgtagt gcggaattta
     2881 ctgcccggcc aatcgactcc atattgtctt caatacgcag cagaaaacag gaaaccagtt
     2941 cgccgcgctg ctgcttaccg cagttgagga atgttggcgt ggctggctga aagcgtcctg
     3001 acagcatctc atcggtgagc tgcgtcgcca gtgtttcatc gccttgtgcc agcgtcagcg
     3061 ccaccatcgt gacgcgatcg gcaaaatctt ccagataacg cttaccgtcg aatgtcttca
     3121 acgtatagct ggtgtaaaac ttccatgctc ctaggaatgt ctggaaacga aaaccgctgg
     3181 cgtgcgcgtg ggcaaacagc gtaatgacaa aatcgcgaga gtagcgatta aggacacttt
     3241 catcatagta accttcgtta accagccagt tcaggcgctg ctgctggcta ctgaaggcca
     3301 cactgttcgg acgcacatgc gtcgtcataa aggcgtctac tgcctggcga tctttatcga
     3361 actgaatgcg acctgcgcta tcgtaaaggt taagcatcgc attcagagcg tgataatcca
     3421 tcgtttcctg cgtcaggcgt tctgcggttg tcgttgccaa aattcagtta ctcctttacg
     3481 aacgttttcg atatcgcttt gcgtacccat gagttcaaag cggtacagcc acggcacgcc
     3541 gcatttccgg gcaatcacat ctccggcgcg gccatatgcc tcaccaaagt tgcgattacc
     3601 cgaagcaata acgccgcgca gcaacgcccg gttatgctcg tcgtttaaaa agcgaatgac
     3661 ctgtcgtggc accgcgccag ccgtaccgcc gccgccgtaa gagggcacga tcaggatgta
     3721 aggctcgtct acctgaatcc gttcccgctc attgagcggg atgcgcaccg cgggcagccc
     3781 taaacgttcg ataaaacgct gcgtattttc ggagctgctg gaaaagtaga cgagctggct
     3841 catgcactgg ccgcgtgtgg cgctggatgc agacgattga tcatgtccgg gcggaaacca
     3901 gaccagctaa gatcgcctgc aatcacaacc ggcaactgac gaaagccctg agcacgcagc
     3961 gcttctgccg cttcaggaac gcgatcgaca ttaatcattt caaaatcaaa gccccggttt
     4021 tccatcgccc gtttagtggc gtggcactga acgcaatcgt tacgagtgta aatagtaatg
     4081 cgcatgattc gtatttccgt ttaaaatgaa gatacggcgc gatgatacgc gtcgggttgt
     4141 ctctctgttg atacagagat actagatgta gttgaaaaaa gattcaacca cacaatatat
     4201 agcaaataat ggtcagggtt accctggggc gagcgtgacg gggtaggggg atttttgtga
     4261 ttcaccaggc aaaaagaaac cccgaagcca ggcttcgggg tcaaagacgc gtatttatta
     4321 tcatttttgc actacgattt gcgcatgctt aacagtgcgc cgataaaaat acccaccgct
     4381 gccgctgtac ccacactaca ccagggtctt tcacgaacaa aagaatccgc gcagccaacg
     4441 gcatcgcgcg cggcttgctg gacgcgagta cgaccatgca ttcgcgccct ggtttctttc
     4501 agtagagcct gcgctttact acgagcggct tcggcttccc ctttggcgtc gctgccccag
     4561 gacttcaata cagattcgag actgtcagct aattgattga catcattctg gatatcctgc
     4621 acgccatcat caacgtcgtt gcggttcggt cggttaaaca tatgatcccc cattagaatt
     4681 cgatgtaatt ttcagtgtag accataattt tcttaactga ggttcatcgc ggctttatgg
     4741 acttttctga aagcgttgtg aatgcgcaat caggtaaggt aggcccgcgg ttgataataa
     4801 cgaggatcaa atatgtattt acgaccagac gaggtggcgc gcgtacttga aaaagtcggt
     4861 tttactgtcg atgtggtaac ccagaaagcg tatggttacc gccgtgggga aaattatgtc
     4921 tatgttaatc gcgaagcgcg aatgggacgt accgccctgg tgattcatcc aacattaaaa
     4981 gaacgtagtt caacactggc tgaacccgct tccgatatta aaacttgcga tcattaccag
     5041 caatttccgc tctatttagc gggtgaacgt catgagcatt acggtatccc gcatggcttt
     5101 agttcgcgtg ttgcgcttga acgttatttg aatggtttat ttggcgaagc cagttaaaga
     5161 cgcgactggc gatgccagtc gcgaaaagaa gggtcaggct tttacctgct ggtaacggct
     5221 gactttaaac agtcggcggc aataatcgag gaagtagcca taaaccgccc ccatcaacat
     5281 cgaaacaacg atgtttgaac tgactgccgc cataatctga tgccagtctg cgcccaccac
     5341 taacaagatc gccacataca ccggtgactg gaacgtcaca taagccagga tatccgccag
     5401 attttttatc cagcctgacg ggctaacttt gcgtgccgcg cgcataaaca gatcgcggta
     5461 cataccgtat ggccatgcaa ttaagatgtt caccggaatc gctaccaatc tggaataaaa
     5521 agactgttcg aagctcattc cggagaggaa aacttcaata cacatgttca cgacagaaca
     5581 gtaaacaacc atcgcgaacg tatctgcaac tgcatgacgc aagcgtgact gcggtgagaa
     5641 catgctaatg ctccttaata aaagcgtaat gggatgttaa tggagatgaa agcagtggat
     5701 ctcattgcca tctgcatttt tgcatagcgt atatcgctgg ctatgactaa tcaattagtg
     5761 aaaaattttt atttaatct
//
LOCUS       DAHHTG010000202         5740 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.206, whole genome shotgun sequence.
ACCESSION   DAHHTG010000202 DAHHTG010000000
VERSION     DAHHTG010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5740)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5740)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5740
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.206"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            <1..984
                     /gene="ldhA"
                     /locus_tag="NBI74_004318"
     CDS             <1..984
                     /gene="ldhA"
                     /locus_tag="NBI74_004318"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HCD1934548.1"
                     /translation="LAVYSTKQYDKKYLQQVNESFGFELEFFDFLLTEKTAKTANGCE
                     AVCIFVNDDGSRPVLEELKKHGVKYIALRCAGFNNVDLDAAKELGLKVVRVPAYDPEA
                     VAEHAIGMMMTLNRRIHRAYQRTRDANFSLEGLTGFTMYGKTAGVIGTGKIGVAMLRI
                     LKGFGMRLLAFDPYPSAAALELGVEYVDLPTLFSESDVISLHCPLTPENYHLLNEAAF
                     DQMKNGVMIVNTSRGALIDSQAAIEALKNQKIGSLGMDVYENERDLFFEDKSNDVIQD
                     DVFRRLSACHNVLFTGHQAFLTAEALTSISQTTLQNLSNLEKGETCPNELV"
     gene            1095..1517
                     /gene="hslJ"
                     /locus_tag="NBI74_004319"
     CDS             1095..1517
                     /gene="hslJ"
                     /locus_tag="NBI74_004319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HslJ"
                     /protein_id="HCD1934549.1"
                     /translation="MKKVAALVALSLLMAGCVSSDKIAVTPEQLQHHRFVLESVNGKP
                     VTSDKNPPEISFGEKMMISGSMCNRFSGEGKLSNGELTAKGLAMTRMMCANPQLNELD
                     NTISEMLKEGAQVDLTANQLTLATAKQTLTYKLADLMN"
     gene            complement(1514..1780)
                     /locus_tag="NBI74_004320"
     CDS             complement(1514..1780)
                     /locus_tag="NBI74_004320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015964583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing protein"
                     /protein_id="HCD1934550.1"
                     /translation="MRAAFWVGCAALLLSACSREPVQQATAAHVAPGLKASMSSSGEA
                     NCAMIGGSLSVARQLDGTAIGMCALPNGKRCSEQSLAAGSCGSY"
     gene            2054..5578
                     /gene="nifJ"
                     /locus_tag="NBI74_004321"
     CDS             2054..5578
                     /gene="nifJ"
                     /locus_tag="NBI74_004321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HCD1934551.1"
                     /translation="MITIDGNGAVASVAFRTSEVIAIYPITPSSTMAEQADAWAGNGL
                     KNVWGDTPRVVEMQSEAGAIATVHGALQTGALSTSFTSSQGLLLMIPTLYKLAGELTP
                     FVLHVAARTVATHALSIFGDHSDVMAVRQTGCAMLCAANVQEAQDFALISHIATLKSR
                     VPFIHFFDGFRTSHEINKIVPLADDTILELMPQAEIDAHRARALNPEHPVIRGTSANP
                     DTYFQSREATNPWYNAVYDHVEQAMNDFAAATGRQYQPFEYYGHPQAERVIILMGSAI
                     GTSEEVVDELLTRGEKVGVLKVRLYRPFSAKHLLQALPGSVRNVAVLDRTKEPGAQAE
                     PLYLDVMTALAEAFNNGERETLPRVIGGRYGLSSKEFGPDCVLAVFAELNAAKPKARF
                     TVGIYDDVTNLSLPLPENTLPNSAKLEALFYGLGSDGSVSATKNNIKIIGNSTPWYAQ
                     GYFVYDSKKAGGLTVSHLRVSEQPIRSAYLISQADFVGCHQLQFIDKYQMAERLKPGG
                     IFLLNTPYSAAEVWSRLPQEVQAVLNQKKARFYVINAAKIARECGLAARINTVMQMAF
                     FHLTQILPGDSALAELQGAIAKSYSSKGQDLVERNWQALALARESVEEVPLQPVNPHS
                     ANRPPVVSDAAPDFVKTVTAAMLAGLGDALPVSALPPDGTWPMGTTRWEKRNIAEEIP
                     IWKEELCTQCNHCVAACPHSAIRAKVVPPEAMENAPASLHSLDVKSRDMRGQKYVLQV
                     APEDCTGCNLCVEVCPAKDRQNPEIKAINMMSRLEHVEEEKINYDFFLNLPEIDRSKL
                     ERIDIRTSQLITPLFEYSGACSGCGETPYIKLLTQLYGDRMLIANATGCSSIYGGNLP
                     STPYTTDANGRGPAWANSLFEDNAEFGLGFRLTVDQHRVRVLRLLDQFADKIPTELLT
                     ALKSDATPEVRREQVAALRQQLNDVAEAHELLRDADALVEKSIWLIGGDGWAYDIGFG
                     GLDHVLSLTENVNILVLDTQCYSNTGGQASKATPLGAVTKFGEHGKRKARKDLGVSMM
                     MYGHVYVAQISLGAQLNQTVKAIQEAEAYPGPSLIIAYSPCEEHGYDLALSHDQMRQL
                     TATGFWPLYRFDPRRADEGKLPLALDSRPPSEALEETLLHEQRFRRLNSQQPEVAEQL
                     WKDAAADLQKRYDFLAQMAGKAEKSNTD"
BASE COUNT         1439 a         1483 c         1485 g         1333 t
ORIGIN      
        1 ctcgccgttt atagcacaaa acagtacgac aagaagtacc tgcaacaggt gaacgagtcc
       61 tttggctttg agctggaatt ttttgacttt ctgctgacgg aaaaaaccgc taaaactgcc
      121 aatggctgcg aagcggtatg tattttcgta aacgatgacg gcagccgccc ggtgctggaa
      181 gagctgaaaa agcacggcgt taaatatatc gccctgcgct gtgccggttt caataacgtc
      241 gaccttgacg cggcaaaaga actggggctc aaagtagtcc gtgttccagc ctatgatcca
      301 gaggccgtag ctgaacacgc catcggtatg atgatgacgc tgaaccgccg tattcaccgc
      361 gcgtatcagc gtacccgtga cgctaacttc tctctggaag gtctgaccgg ctttactatg
      421 tatggcaaaa cggcaggcgt gatcggtacc ggtaaaatcg gtgtggcgat gctgcgcatt
      481 ctgaaaggtt ttggtatgcg tctgctggcg ttcgatccgt atccaagtgc ggcggcgctg
      541 gaactcggtg tggagtatgt cgatctgcca accctgttct ctgaatcaga cgttatctct
      601 ctgcactgcc cgctgacacc ggaaaactac catctgttga acgaagccgc cttcgatcag
      661 atgaaaaatg gcgtgatgat cgtcaatacc agtcgcggtg cattgattga ttctcaggcg
      721 gcaattgaag cgctgaaaaa tcagaaaatt ggttcgttgg gtatggacgt gtatgagaac
      781 gaacgcgatc tgttctttga agataaatcc aacgacgtga tccaggatga cgtattccgt
      841 cgcttgtctg cctgccacaa cgtgctgttt accgggcacc aggcattcct gacagcagaa
      901 gctctgacca gtatttctca gactacgctg caaaacttaa gcaatctgga aaaaggcgaa
      961 acctgcccga acgaactggt ttaatcttgc cgctcccctg cattccaggg gagcttattc
     1021 agataatccc caaagacctt tcatcctcta ttcttaaaat agtcctgaat cagaaacagt
     1081 aattgagaac cacaatgaag aaagtagccg cgcttgttgc gctaagcctg ctgatggcgg
     1141 gatgtgtaag tagtgacaaa attgctgtaa cgccagaaca gctacagcat catcgctttg
     1201 tgctggaaag cgtaaacggt aagcccgtga ccagcgataa aaatccgcca gaaatcagct
     1261 ttggtgaaaa gatgatgatt tccggcagca tgtgtaaccg ctttagcggt gaaggcaaac
     1321 tgtctaatgg tgaactgacc gccaaagggc tggcaatgac ccgtatgatg tgcgctaacc
     1381 cgcagcttaa tgaactcgat aacaccatta gcgaaatgct gaaagaaggt gcacaagtgg
     1441 atctgaccgc gaaccagtta acgctggcga ccgcgaaaca gacattaact tataagctgg
     1501 cggatttaat gaattaatag ctgccacagc tcccggcggc aagtgactgt tcgctacagc
     1561 gtttgccgtt gggtaatgca cacatcccaa tcgccgtacc atccagttga cgggcaacag
     1621 aaagcgaacc gccgatcatt gcacaatttg cttctccact actggacatc gacgctttta
     1681 aacctggcgc tacgtgcgcc gcagtcgcct gctgaacagg ttcacgacta cacgccgaca
     1741 acaataaagc ggcacaccct acccaaaacg ctgctcgcat cttttcttcc tctgatcttc
     1801 aagccaaacg acaccgccat aaataatagg cagcacagag ggcggcgtcg agagctgtcc
     1861 tgcgcgttgc cccgcatttt tactttttta tggctatttt tttgccctct gtttgatcaa
     1921 aacattcatt acgctgatgt gggggacaca aaagcgaaaa tgcagaagaa agccatttgc
     1981 taaaattgaa cgattactac tgggcgcgca gcaatttcgt gcgcccctca tttgcgcaat
     2041 gtaagggtgt catatgatta ctattgacgg taatggcgcg gttgcttcgg tggcgtttcg
     2101 caccagtgaa gttatcgcca tctaccctat tacccccagt tccacgatgg cagaacaggc
     2161 tgatgcatgg gccggaaacg gcttgaaaaa cgtttgggga gacacaccac gcgtggttga
     2221 aatgcagtcg gaagcgggtg ctatcgctac cgtgcatggt gctttgcaaa ctggtgccct
     2281 ctcaacatcg tttacgtcat cgcagggttt gctgctgatg atcccaacgc tgtacaaact
     2341 ggcaggcgaa ctcacaccgt ttgtcctgca tgttgcggca cgtaccgttg ccacacatgc
     2401 actctctatt tttggcgatc attccgacgt tatggcagtg cgccaaacgg gttgcgcgat
     2461 gttgtgtgca gcaaacgtcc aggaagcgca agactttgct ctcatttcgc acattgcgac
     2521 gctgaaaagt cgcgtgccat ttattcattt ctttgatggt ttccgcacat ctcacgaaat
     2581 caataaaatt gtcccgctgg ccgatgacac gattcttgaa ctgatgccgc aggcagaaat
     2641 tgatgctcat cgcgcccggg cactcaaccc ggaacatccg gtgatccgcg gtacgtccgc
     2701 caatcctgac acttatttcc agtctcgcga agccaccaac ccatggtaca acgcggtcta
     2761 tgaccatgtt gaacaggcga tgaatgattt cgctgccgcg acaggtcgtc agtatcagcc
     2821 gtttgagtat tacgggcatc cgcaagcgga acgggtgatt atcctgatgg gctctgccat
     2881 tggcaccagt gaagaagtgg ttgatgaatt gctgacccgt ggcgaaaaag tcggcgtgct
     2941 gaaagttcgc ctgtaccgcc ccttctccgc caaacacttg ctgcaagctc tgccgggatc
     3001 cgtgcgcaac gtggcggtac tggacagaac caaagaaccc ggtgcccagg cagaaccgct
     3061 ctatctggat gtgatgactg cactggcaga agcctttaat aatggcgagc gcgaaaccct
     3121 gccccgtgtc attggtgggc gctatggtct ttcatccaaa gaatttggcc cggactgtgt
     3181 actggcggta tttgccgagc tcaacgcggc taaaccgaaa gcgcgcttta cggttggtat
     3241 ttacgatgat gtaaccaatc tgtcactgcc gttgccggaa aacaccctac caaactcggc
     3301 gaaactggaa gccttgtttt atggccttgg tagtgatggc agcgtttccg cgaccaaaaa
     3361 caatatcaag attatcggaa attccacgcc gtggtacgca cagggctatt ttgtttacga
     3421 ctccaaaaag gcgggcggcc tgacggtttc tcaccttcgc gtgagcgaac agccgattcg
     3481 ttccgcttat ctcatttccc aggctgattt tgttggctgc caccagttgc agtttatcga
     3541 taaatatcag atggctgagc gtttaaaacc tggcggcatt ttcctgctca acacgccgta
     3601 cagcgcagct gaagtgtggt cgcgcttgcc gcaggaagtt caggccgtgc taaaccagaa
     3661 aaaagcgcgc ttctatgtga ttaacgcggc gaaaatcgcc cgcgaatgtg gcctggcggc
     3721 ccgtattaat accgtcatgc agatggcttt tttccatctg acgcagattc tgcctggcga
     3781 tagtgccctc gcagaattgc agggtgcgat tgccaaaagt tacagtagca aaggccagga
     3841 tctggtggaa cgcaactggc aggctctggc gctggcacgt gaatccgtag aagaagttcc
     3901 actgcagcca gttaatccgc atagcgccaa tcgaccgccg gtggtttccg atgccgctcc
     3961 tgatttcgtg aaaaccgtaa ccgctgcgat gctcgccggg cttggcgacg ccctccccgt
     4021 ttcggcgctg ccgccagacg gcacctggcc gatgggcacc acgcgctggg aaaaacgcaa
     4081 tatcgccgaa gagatcccca tctggaaaga ggaactctgt acccaatgta accactgtgt
     4141 cgccgcttgc ccacactcag ctattcgcgc aaaagtggta ccgcctgaag cgatggaaaa
     4201 cgcccctgcc agcctgcatt cgctggatgt gaaatcgcgt gatatgcgcg ggcagaaata
     4261 tgtcttgcag gtggctccgg aagattgcac cggttgtaac ctgtgcgtcg aagtttgccc
     4321 ggcgaaagac cgtcagaatc cagagattaa agccatcaat atgatgtcgc gcctggaaca
     4381 tgtcgaagaa gagaaaatca attacgattt cttcctcaac ctgccagaaa tcgatcgtag
     4441 caaactggaa cgtattgata ttcgtacatc gcagctaatc acgccgctgt ttgaatattc
     4501 cggtgcttgc tccggttgtg gcgagacgcc gtatattaaa ttgctgactc agctctatgg
     4561 cgaccggatg ttgatcgcta atgccactgg ctgttcttca atatacggcg gtaacctgcc
     4621 ttcaacacca tataccaccg atgccaacgg tcgtggaccg gcatgggcga actcgctgtt
     4681 tgaagataac gccgaatttg gccttggttt ccgcctgacg gtcgatcaac accgtgtccg
     4741 cgtgctgcgt ctgctcgatc aatttgccga taaaatcccg acggaattac tgactgcgtt
     4801 gaaatcagac gccacgccag aggttcgtcg tgaacaggta gcagctttac gccagcaact
     4861 caacgatgtt gccgaagcac atgaactgct gcgtgacgca gatgcactgg tggaaaaatc
     4921 aatctggctg attggtggcg atggctgggc ttacgatatc ggctttggcg gtctggatca
     4981 tgtattgagt ttgacggaaa acgtcaacat tctggtgctg gatacgcaat gctattccaa
     5041 caccggaggt caggcatcga aagcgacacc gctgggtgca gtaactaagt ttggcgagca
     5101 tggcaaacgt aaagcgcgta aagatcttgg cgtcagtatg atgatgtacg gtcatgttta
     5161 tgtggcgcag atttctctcg gcgcgcagct gaaccagacg gtgaaagcga ttcaggaagc
     5221 ggaagcgtat ccggggccat cgctgatcat cgcttatagc ccgtgtgaag agcatggtta
     5281 cgatctggca ctcagccacg atcagatgcg ccaactcact gctaccggct tctggccgtt
     5341 atatcgcttc gatcctcgtc gtgccgatga aggcaaactg ccgctggcgc tggattcacg
     5401 cccgccgtca gaagcacttg aagaaacgtt acttcacgag cagcgtttcc gtcgtctgaa
     5461 ttcgcagcag ccagaagtgg cagaacagtt atggaaagat gctgcagctg atttgcaaaa
     5521 acgctatgac ttcctggcac aaatggccgg aaaagcggaa aaaagcaaca ccgattaaat
     5581 gctctggata aggattatcc aattcaaaaa aaaagcccgg acgactgttc gggcttgtct
     5641 tcttatgtgt tggaaaatca gtagcaatgc aatggcccaa cagaaaataa aatctgtgac
     5701 aataaaggca tataacccgc gcagaataac gtatagaaaa
//
LOCUS       DAHHTG010000203         5683 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.207, whole genome shotgun sequence.
ACCESSION   DAHHTG010000203 DAHHTG010000000
VERSION     DAHHTG010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5683)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5683)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5683
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.207"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            68..2479
                     /locus_tag="NBI74_004322"
     CDS             68..2479
                     /locus_tag="NBI74_004322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001177147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HCD1934552.1"
                     /translation="MELSDGNNAKNIDLSYFMNASGAAPGEYTVDVIMNGKIVDSQIK
                     IDFTEQDNELTARLTPQQLERWGIDTNKLSVSSPSAYQQNIAYIIQGATEKFDANNQK
                     LILNIPQSYLKPQDWLSTPPHLWDEGMPALMVNYLFNGIKQNNNGYGSRSQFLSLDSS
                     LNLGGWRLRHNGNWSTNSYNKESHWQPVSVYLQHDYSFLQGGQFTVGQTSTDGAIFDS
                     FPFEGAQFSSDDGMIAPELSQYSPVVRGIAYSQAQVSVKQNGVVIYQKNVPPGPFELR
                     DFNQIFTGDLEVEIREADGTIRHFTQATAVLPILQRQGRLRYNLAFGKYRSSSTLNNS
                     VSEPQFVQSSAAIGLPDEYTFYGGGIKADNYAAVLLGLGKYSDFFGAFSLDVTHARSQ
                     FSQNYKSFGKQQGQSYRFMYSRGFGETNTTLNITGYRYATRGYYDFDELQQIQSGFVD
                     EKNINSYHQRSRISTTISQDLQDWGQFYLSASKDQYWDTADGYNLSASYSLPFRYISA
                     MLSLGYNKSPYYHEADKSLFLSVSVPLNSLLNGNNLFLTTNTITNNGQVQQQVGLNGS
                     SQDGEFNYAVAQGWQNQNRGESGNINLNYRGPYAQMNGGYAWQKESSQWTYGISGGIT
                     LHPHGLTLSQPLSLDSASALVQARDAASVKVLNGSGIYTDWRGYAVVPYLNPYNRNQI
                     SLDVNSVKDNVELLNTDVTVIPSRGALVSAPFKVNVGNKAIITLIQKNGEPVPFGSVV
                     TLDSENSINSSIVADQGQVYMSGLPEEDTLIAQWGEEASQKCKTNYKLQNKTAKFSEL
                     TLQCQ"
     gene            complement(2589..2861)
                     /locus_tag="NBI74_004323"
     CDS             complement(2589..2861)
                     /locus_tag="NBI74_004323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HCD1934553.1"
                     /translation="MEIREGHNKFYINDEQGKQIAEIVFVPTGENLAIIEHTDVDESL
                     KGQGIGKQLVAKVVEKMRREKRKIIPLCPFAKHEFDKIREYDDIRS"
     gene            complement(2873..3103)
                     /locus_tag="NBI74_004324"
     CDS             complement(2873..3103)
                     /locus_tag="NBI74_004324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(4Fe-4S)-binding protein"
                     /protein_id="HCD1934554.1"
                     /translation="MDQALLDGGYRCYTGEKIDVYFNTAICQHSGNCVRGNGKLFNLK
                     RKPWIMPDEVDVTTVVKVIDTCPSGALKYRHK"
     gene            3284..4915
                     /gene="dcuS"
                     /locus_tag="NBI74_004325"
     CDS             3284..4915
                     /gene="dcuS"
                     /locus_tag="NBI74_004325"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     DcuS"
                     /protein_id="HCD1934555.1"
                     /translation="MRHSLPYRMLRKRPMKLSTTVILMVSAVLFSVLLVVHLIYFSQI
                     SDMTRDGLANKALAVARTLADSPEIRQGLQKKPQESGIQAIAEAVRKRNDLLFIVVTD
                     MHSLRYSHPEAQRIGQPFKGDDILKALNGEENVAINRGFLAQALRVFTPIYDENHKQI
                     GVVAIGLELSRVTQQINDSRWSIIWSVLFGMLVGLIGTCILVKVLKKILFGLEPYEIS
                     TLFEQRQAMLQSIKEGVVAVDDRGEVTLINDAAQELLNYRKSQDDEKLSTLSHSWSQV
                     VDVSEVLRDGTPRRDEEITIKDRLLLINTVPVRSNGVIIGAISTFRDKTEVRKLMQRL
                     DGLVNYADALRERSHEFMNKLHVILGLLHLKSYKQLEDYILKTANNYQEEIGSLLGKI
                     KSPVIAGFLISKINRATDLGHTLILNSESQLPDSGSEDQVATLITTLGNLIENALEAL
                     GPEPGGEISVTLHYRHGWLHCEVNDDGPGIAPDKIDHIFDKGVSTKGSERGVGLALVK
                     QQVENLGGSIAVESEPGIFTQFFVQIPWDGERSNR"
     gene            4912..5631
                     /gene="dcuR"
                     /locus_tag="NBI74_004326"
     CDS             4912..5631
                     /gene="dcuR"
                     /locus_tag="NBI74_004326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator DcuR"
                     /protein_id="HCD1934556.1"
                     /translation="MINVLIIDDDAMVAELNRRYVAQIPGFQCCGTASTLEKAKEIIF
                     NSDTPIDLILLDIYMQKENGLDLLPVLHNARCKSDVIVISSAADAATIKDSLHYGVVD
                     YLIKPFQASRFEEALTGWRQKKMALEKHQYYDQAELDQLIHGSSSNEQDPRRLPKGLT
                     PQTLRTLCQWIDAHQDYEFSTDELANEVNISRVSCRKYLIWLVNCHILFTSIHYGVTG
                     RPVYRYRIQAEHYSLLKQYCQ"
BASE COUNT         1565 a         1302 c         1291 g         1525 t
ORIGIN      
        1 gttcagttgg ggagctgcgt ctcccgttcc tggtcataat aatgagttat catttaaccc
       61 tgattttctg gaattatctg atggtaacaa tgctaaaaac attgacctca gttattttat
      121 gaatgcgagt ggagcggcac caggagaata tactgttgac gttattatga atggtaaaat
      181 tgttgacagt caaattaaaa tcgattttac cgaacaagat aatgagctaa ccgccagact
      241 gacgcctcaa cagttggaac gttggggaat tgatacaaat aagctctccg tatcttcccc
      301 atctgcttac cagcaaaata ttgcatatat tattcagggc gcaacagaga aattcgatgc
      361 gaacaaccaa aagctaatat tgaatatacc acaaagctac ctcaaacctc aggattggtt
      421 aagtactccg ccccatctct gggatgaagg tatgccagcg ctaatggtta actatctatt
      481 taacggaatt aagcaaaata ataatggcta cggtagccgt agtcagtttt tatctctgga
      541 tagttctctg aaccttggtg gatggcgcct tcgccataac ggtaactggt caacaaactc
      601 atataataaa gagagccact ggcaacccgt tagtgtttat ctacagcatg attattcttt
      661 cctgcaggga gggcaattta ctgtagggca aacctcaacg gatggtgcga tatttgacag
      721 ttttcctttt gaaggagcac agttttcatc tgatgatggc atgattgccc cagaactcag
      781 ccaatatagt cctgttgtcc gtggcattgc ttattctcaa gcacaggtat ccgtcaagca
      841 aaacggagtt gtcatttatc aaaagaacgt cccacctgga ccgtttgaat tacgcgattt
      901 taaccaaata ttcaccggtg acctggaggt ggaaatccgt gaggcagatg gcactattcg
      961 ccattttact caggcgacgg ccgttttacc gattctgcaa cgtcaggggc gcttacgcta
     1021 taacctggca ttcgggaaat atcgctcaag ttctactttg aataatagtg tgtctgagcc
     1081 tcagtttgtt cagtcttcag cagcaatcgg attacctgat gaatatacat tttatggtgg
     1141 tggtatcaaa gcagacaact atgcagcagt tctgcttggc ctgggtaaat actcagattt
     1201 ttttggagcg ttctccctgg atgtcactca tgctcgctca caattttcgc agaattataa
     1261 gtctttcgga aaacagcaag gacagtctta ccgctttatg tattcgcgtg gttttggtga
     1321 gacgaatacg acattgaata ttaccggcta tcgttatgcc acacgcggtt actacgattt
     1381 tgatgagtta caacaaattc agagtggatt cgttgatgag aaaaatataa acagttatca
     1441 tcaacgttcg cgtatatcaa caacgataag tcaagatctt caggattggg gacaattcta
     1501 cctctcagca agtaaagatc agtactggga taccgctgat ggatataact tatctgcgag
     1561 ttactcgctg ccattccgct atatctcggc aatgctatcg ctcgggtata ataaaagccc
     1621 gtattaccac gaggctgata aatcgctgtt tttatctgtg tctgtgccgc tcaactcatt
     1681 gctgaatgga aataacctgt tcttaaccac gaataccatt acgaataatg gtcaggtgca
     1741 acagcaggtt ggattaaacg gctcctcaca ggatggtgag tttaactatg ccgtggcgca
     1801 aggctggcag aatcaaaaca gaggagagtc aggtaacatt aaccttaact atcgtggacc
     1861 ttatgcacaa atgaacggcg gctatgcctg gcaaaaagaa tccagtcaat ggacatacgg
     1921 aattagcggt ggtattacgt tgcatccaca tggattaacc ttatcccagc cattaagtct
     1981 ggacagtgcc agtgctctgg ttcaggctcg tgatgccgcc agcgtaaaag tattgaacgg
     2041 gtctggtatt tatacggact ggcgtggata tgctgtggtc ccatatttga atccctataa
     2101 ccgaaatcaa atttcacttg atgttaatag tgttaaggat aatgttgagc tgctaaatac
     2161 agatgttact gtcatccctt ctcgaggagc tttagtttcc gctccattta aagtcaatgt
     2221 cggtaataag gcaataatta ccttgataca gaagaatggt gagcccgtac cgtttggtag
     2281 tgtggtgaca ttagattctg aaaatagcat taacagcagt attgttgctg atcaaggaca
     2341 ggtgtatatg tcagggttac ctgaagagga tacacttatt gcacaatggg gtgaggaagc
     2401 ttctcaaaag tgtaaaacaa attacaaatt gcaaaataaa acagcgaaat tcagtgaatt
     2461 aactttacaa tgccaatagt cggtagttct tagattaata accctgcata tgcggggtta
     2521 ttttcatcta acatattaac aacatcattc cagatttgta ttacttccga cggacacaaa
     2581 tcatcttttc aactgcgaat atcatcatac tcccgtattt tatcaaattc gtgtttcgca
     2641 aatgggcata gtgggataat ttttcgtttt tcccgacgca ttttttccac gactttcgca
     2701 accagctgtt taccgatccc ttgccctttc aggctttcat cgacatcggt atgttcgata
     2761 atcgctaaat tctctccggt cggcacaaag acaatttcag cgatttgctt gccctgttcg
     2821 tcattaatgt aaaatttatt gtggccttcg cgtatttcca ttttaccctc gcttatttat
     2881 gacggtattt cagcgcaccg ctcgggcacg tatcaatcac tttcaccaca gtggtgacgt
     2941 caacttcatc cggcatgatc cacggcttac gtttgagatt aaataacttg ccgttgccac
     3001 gtacgcaatt gccagaatgc tgacatatcg cagtgttgaa atagacatcg atcttttcgc
     3061 cggtataaca gcgataaccc ccgtccagta gcgcctgatc catgaccttc cctctctcat
     3121 cagtatagtt ttcactataa gcatagcccc ggaatcttca tacagcactg gcagtttccg
     3181 ttgccagagt ctcttcccct atatagccaa tcattcttac tccctttgaa ttacccgcct
     3241 catcagagat aatgcttaag aaatttgtca cacaaggaag ctgatgagac attcattgcc
     3301 ctaccgtatg ttacgcaaac gtccgatgaa attgagtacc actgtgatct taatggtcag
     3361 cgcggtactg ttctcggtgc tattggtggt gcatctgatt tacttctcgc aaatcagtga
     3421 tatgacgcga gacgggctag ctaacaaggc tctggcagta gcgcgtaccc tcgccgactc
     3481 gccggaaatc cgtcagggct tgcagaaaaa accgcaggag agtggaatcc aggccatcgc
     3541 ggaagccgtg cgcaaacgca acgatctgct gtttattgtc gttaccgata tgcacagtct
     3601 tcgctactcg catcctgaag cccagcgtat tggtcagcca tttaaaggtg atgacatcct
     3661 taaagcgctg aatggcgaag aaaatgtcgc tatcaatcgc ggttttctgg cgcaggcttt
     3721 acgcgtattt acccccatct acgatgaaaa tcataaacaa attggcgtag tggcgatcgg
     3781 ccttgagtta agccgcgtga cccaacagat caatgacagt cgctggagca ttatctggtc
     3841 ggtactattt ggcatgctgg tcggactgat cggcacatgc attctggtta aagtactgaa
     3901 aaaaatcctt ttcggcctgg aaccctacga aatctccaca ctgtttgagc aacgccaggc
     3961 catgttgcag tccatcaaag aaggcgtcgt cgccgtggac gatcgcggcg aggtcaccct
     4021 gatcaacgat gccgcacaag aattgctgaa ttaccgtaag tcgcaggacg atgagaaact
     4081 gtcgacgcta agccactcgt ggtcacaggt ggtagatgtc tcggaagtgt tacgcgacgg
     4141 aaccccacgc cgcgacgaag agattacgat taaagaccga ctattactga tcaacaccgt
     4201 tccggtgcgc agtaatggcg ttatcatcgg tgccatttca accttcaggg acaaaactga
     4261 agtacgtaaa ctgatgcagc gactcgacgg tctggtcaac tatgctgacg cacttcgtga
     4321 acgatcccac gaatttatga ataaattgca tgtgattctc ggattattgc atctgaagag
     4381 ttataagcag ttggaagatt acattctcaa aacagccaat aactatcagg aagagattgg
     4441 ctctctgctg ggtaagatca aatctccggt tatcgctggt tttttaatca gcaagattaa
     4501 ccgcgcgacc gatttaggcc atacgctgat tttaaacagt gaaagccagc tgccagacag
     4561 cggtagtgag gatcaggtcg cgacgctgat taccacgttg ggaaatctga tagaaaacgc
     4621 gctggaggca ttagggccgg aacccggtgg cgaaattagc gtaacattgc actaccgtca
     4681 cggctggctg cactgcgaag ttaacgatga tggaccgggg atcgcacccg acaaaatcga
     4741 tcacattttt gacaaaggtg tctcgacaaa aggaagcgag cgaggcgtcg gtttagcact
     4801 tgtcaaacaa caggtagaaa atctcggcgg cagcatcgcc gtggaatcgg aacccgggat
     4861 tttcacacaa ttttttgtcc agataccctg ggacggggag aggtcgaaca gatgatcaat
     4921 gtattaatta tcgatgacga cgcaatggtc gcggagctga atcgccgata cgtggcacaa
     4981 atcccgggct ttcaatgctg tggaacggct tcgacgctgg agaaagccaa agagattatc
     5041 ttcaatagcg atacgcctat cgacctgata ttgctagata tctatatgca aaaagagaac
     5101 gggctcgatt tactgcctgt cctgcataac gcgcgttgca aaagtgatgt gattgtcatc
     5161 tcctccgctg ccgatgcagc aaccatcaaa gattcgctgc attacggtgt tgtggattac
     5221 ctgatcaaac ccttccaggc ttcccgcttt gaagaggcgc tcaccggctg gcggcaaaag
     5281 aaaatggcgc tggaaaaaca tcagtattat gaccaggccg agcttgatca gctaatccac
     5341 ggcagcagct ccaacgagca ggatcctcgt cgcttgccaa aaggcttaac gccgcagacg
     5401 ttacgcacgc tgtgccagtg gattgacgcg catcaggact atgagttttc aaccgacgag
     5461 ctggctaacg aggttaacat ctcgcgtgtt tcctgccgta aataccttat ctggctggtt
     5521 aactgccaca tcttgttcac cagtatccat tatggcgtca cggggcgtcc ggtttaccgt
     5581 taccgtattc aggcagaaca ctactcacta ctgaaacaat attgccaata acggatcgct
     5641 ggttatctgt aagtaataat tattatcgta atgcaaaata ccc
//
LOCUS       DAHHTG010000204         5671 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.208, whole genome shotgun sequence.
ACCESSION   DAHHTG010000204 DAHHTG010000000
VERSION     DAHHTG010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5671)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5671)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5671
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.208"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            47..2425
                     /gene="vgrG"
                     /locus_tag="NBI74_004327"
     CDS             47..2425
                     /gene="vgrG"
                     /locus_tag="NBI74_004327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001574081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
                     /protein_id="HCD1934557.1"
                     /translation="MDVPVLNFDHSHHKLKIRGLQSPVDVLTFEGREQLSTPFRYDIQ
                     FTSTDKAIAPESVLMQDGAFSLTAPPVQGMPVQTALRTLHGVITSFKHLSSSQDEARY
                     EVRLEPRMALLTRSRQNAIYQNLTVPQIVEKILRERHQMRGQDFVFNLKSAYPSREQV
                     MQYGEDDLTFVSRLLSEVGIWFRFATDARLKIEVVEFYDDQSGYERGLTLPLRHPSGL
                     FDGETEAVWGLNTAYSVVEKNVTTRDYNYRTATAEMMTEQHDATGGDNTTYGEAYHYA
                     DNFLQKGDKEAAESGAFYARIRHERYLNEQAILKGQSTSSLLMPGLEIRVQGDDAPAV
                     FRKGVLITGVTASAARDRSYELTFTAIPYSELYGYRPALIPCPVMAGTLPARVTSTVK
                     NDIYAHIDKDGRYRVNLDFDRDTWKPGYESLWVRQSRPYAGDTYGLHLPLLAGTEVSI
                     AFEEGNPDRPYIAGVKHDSAHTDHVTIQNYKRNVLRTPANNKIRLDDERGKEHIKVST
                     EYGGKSQLNLGHLVDAGKQQRGEGFELRTDLWGAVRAKKGIFISADAQDKAQGQVREM
                     ADIISELNSLSDKIQKLSDDAATANADPADMAAQIALITSRINDLTASVILMHAPKGV
                     AVASGEHLQLAAVKNLQINAGNNADIGVVKNMFIGVGRALSVFVRKAGIRLIANKGAV
                     SVQAQHDLMELLAKKSIEIVSTEDEIKITAKKKITINGGGSYIRIEGSGIEPGTPGDY
                     NVKAVHYGRQPKASEKVPMPEFPILSAVDSSDFCLECLLNAIKNDDAVVEGV"
     gene            2428..3249
                     /locus_tag="NBI74_004328"
     CDS             2428..3249
                     /locus_tag="NBI74_004328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000200148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4123 domain-containing protein"
                     /protein_id="HCD1934558.1"
                     /translation="MSSEQQLADHWLKRNETLNAFILFEGGALSQEMLQIMMEHYKYR
                     RISLYQLPDLKKVAAYGPWLVRIESEKDLAMVLVGKLPVAGITFSELQLEPLASRLAV
                     GCMAKTPDGREVLLRYYTPRVLKQLAARPDMDWHPVMFSQIESWWMLEDTRWQRLNMP
                     LTVANNSDETVEIITLDEPLWQKIADRGDVSALLREWRTMKTSEHFPPCIQRNMVEKA
                     LNKAQVVGLTKPLDQKIYALSYLNGKKAYLESAEFQVMLEKVKRNEISLSDLGRQ"
     gene            3293..3820
                     /locus_tag="NBI74_004329"
     CDS             3293..3820
                     /locus_tag="NBI74_004329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000522562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3304 domain-containing protein"
                     /protein_id="HCD1934559.1"
                     /translation="MGLFTRLEKFDQKLTRGYARWGRWVWRMLIAIPVAYFLLCVGIS
                     IWGAPTGGVILVIHSELDRPILGFSVNGMAGANAFAHGGGSTTCCGDIRGKEAEVVWT
                     LSTTRAQYNTGLREEVRRITLPLPERKHGQDFLHVHFLPGDKVFLGWSEGAGSPYEKR
                     KEPSYPSRKNQEAQP"
     gene            3817..>5671
                     /locus_tag="NBI74_004330"
     CDS             3817..>5671
                     /locus_tag="NBI74_004330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001525521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2235 domain-containing protein"
                     /protein_id="HCD1934560.1"
                     /translation="MISESVISAAVRAQQSEDGKVGNCGRIWHVAFFFDGVGRNIDRD
                     ATDCRLSNIARLFRAYPDEQKNTGDTCYNKFYFSGMGTAYHDDSVDNIHALMDIGLDN
                     FLEDMKSLPEDTISDAGESVIKGEKKWTDVLGNLLEDLNNPVEWVKGIGKMAAKSVGK
                     AGIESTPWLRDSEAMSAYFMTGEPVRLEAAKRLFKKFYTENMKGDVPIKKISVSLYGF
                     DLGATLARKFLDNFLKEVCLKNKEKKHQYKNALVDIAFIGLFDCSRHSPTSSNNGLDY
                     FFMWLGLPGKMIGSVLGEKAIDQDSPLPDVVNNALHLVAAHERRPWRGLYLLGKKTDT
                     ANKKYREELLPGCSEDIGGGLKPDEQKPSAELCRVALYNMYHAACQAGVPFPDFDTLD
                     QYDTKVASYFIMNDAVQGGSVNYWVSRYQREEVKDKVFSEAMQEKYLDNYFLWLGEQY
                     YMYQYERERLDKELTSAHRGKISDYGPLAGTGINPNTNADDIKSQISELDSLWGWLND
                     VKSVAIGLRNDFIVNPRKNDSRIKLKPQVYNAAVNRAVKFLEFSRAVYNDKNLPSSSS
                     LTANTLYSFFVHDIQKVDHTPSISEDFFLRRSSEIPDADEPPGNKDDGKKHT"
BASE COUNT         1548 a         1253 c         1545 g         1325 t
ORIGIN      
        1 aacggctcgt tattctttgt tagataaatg aaggagtaca aagaaaatgg atgttcctgt
       61 tctgaatttc gaccactcac accacaagct caaaattcgc ggcctgcaaa gtccggtgga
      121 tgtgctgacc ttcgaaggcc gcgaacaact gagtaccccg ttccgctacg acattcagtt
      181 taccagtacc gataaagcca tcgcgccgga gtcggtgctg atgcaggacg gcgcattcag
      241 cctgacggca ccaccggtac agggaatgcc ggtgcagact gccctgcgca ccctgcacgg
      301 cgtcattacc agctttaaac acctctcctc gtcacaggac gaagcccgtt acgaagtgcg
      361 ccttgaaccc cgtatggcgc tgcttacccg cagccgccag aacgccatct accagaatct
      421 gacggtgccg caaatcgtcg aaaaaatcct gcgcgagcgg caccagatgc gcggtcagga
      481 cttcgtgttt aacctcaaaa gcgcataccc gtcccgcgaa caggtgatgc agtacggtga
      541 ggacgacctg acgtttgtct cacgcctgct gtcggaagtg ggtatctggt tccgctttgc
      601 caccgacgcc cggctgaaaa tcgaagttgt cgagttttat gacgaccaga gcggctacga
      661 gcgcgggctg acactgccgc ttcgccatcc ttcagggctg tttgatggtg aaacggaagc
      721 cgtatggggg ctgaacacgg catacagcgt ggtggagaaa aacgtcacca cgcgggacta
      781 caactaccgc accgccacag cagagatgat gacagagcag catgacgcca ccgggggcga
      841 taacaccacc tatggcgaag cctaccacta cgctgacaac ttcctgcaaa aaggcgacaa
      901 ggaggcagcg gaaagcggcg cgttttatgc ccgtatccgc cacgaacgct acctgaatga
      961 acaggccatt ctcaaaggcc agagcaccag ttcactgctg atgccggggc tggaaatcag
     1021 ggttcagggc gacgacgcgc ctgcggtatt tcgcaagggc gtactgatta ccggcgtgac
     1081 ggccagcgcg gcgcgtgacc gcagctatga gctgacgttt accgccatcc cgtactcaga
     1141 gctctacggc taccgcccgg cgcttatccc ctgccccgtg atggccggca cgcttccggc
     1201 gcgggtcacc agcacggtga agaacgatat ctatgcccat atcgacaagg acgggcgcta
     1261 ccgggtcaac ctcgactttg accgcgatac gtggaaaccg ggttatgaaa gcctgtgggt
     1321 acgccagtcg cgcccgtatg cgggagacac ctacggcctg cacctgccgc tgctggcggg
     1381 aacggaagtc agtatcgcct ttgaggaagg caacccggac cgaccttata ttgcaggcgt
     1441 aaaacacgac tcggcacaca ctgaccatgt gaccattcag aactacaagc gcaacgtgct
     1501 acgcaccccg gcgaacaaca aaatccgcct cgacgatgag cgcggcaaag agcatatcaa
     1561 ggtcagtacc gagtacggcg gcaagagcca gctcaacctc ggtcatctgg tggatgccgg
     1621 gaaacagcag cgcggggaag gttttgaact gcgtaccgat ctgtggggtg cagtacgggc
     1681 aaagaaaggc atctttatca gcgccgacgc gcaggacaag gcgcagggtc aggtgcgcga
     1741 aatggccgac attatcagcg aactgaacag cctgagcgat aaaatccaga aactgtctga
     1801 cgatgcggcc acggcgaacg ccgatccggc ggatatggcc gcgcagatag cattgataac
     1861 cagccggata aatgacctga ccgcctcggt gattttgatg cacgcaccga aaggcgtggc
     1921 ggtcgccagc ggcgagcacc tgcaactggc cgcggtgaaa aacctccaga taaatgcggg
     1981 aaacaacgcc gatatcggcg tagtgaagaa tatgtttatc ggcgtgggac gggcgctgag
     2041 cgtgtttgta cgcaaggcgg gtatcaggct gattgccaat aaaggcgcag tatcggtaca
     2101 ggcgcagcac gatctgatgg agttactggc gaaaaaatcg atagagatcg tcagtaccga
     2161 agatgagatt aaaatcaccg cgaagaaaaa aataacgatt aatggtggtg ggagttatat
     2221 ccggatagag ggtagcggca ttgagccggg aacgccgggg gattataacg tcaaagcggt
     2281 acattacggg cgtcagccta aagcctcgga gaaggttcct atgccagaat ttcccatcct
     2341 ttcagccgtg gactcatccg atttttgtct tgaatgcctg ctgaatgcca tcaaaaacga
     2401 tgacgctgtt gttgaaggag tgtaatcatg tcttcagaac aacaactggc agaccactgg
     2461 cttaagcgaa acgaaacgtt aaacgccttt attttatttg aaggtggagc gctctctcag
     2521 gaaatgcttc agatcatgat ggagcattat aaataccgac gaatttccct gtatcaactg
     2581 cccgatctta aaaaggtagc agcttacggt ccctggttgg ttcgtattga aagtgaaaag
     2641 gatctggcga tggtgcttgt cgggaaacta cctgtcgcag gcattacttt ctccgaattg
     2701 cagctggagc cactggcgtc tcggcttgct gtgggatgta tggcaaaaac gccggatggt
     2761 cgtgaagttc tgctgaggta ctacacgccc cgagtgctga aacaactggc tgcccggcct
     2821 gatatggact ggcatccggt aatgttttct cagatagaat catggtggat gctggaagat
     2881 acgcggtggc aaaggctgaa catgccgctt actgtggcaa ataacagcga tgagacagtg
     2941 gaaatcataa cgcttgatga accgctctgg cagaaaatag cggatcgggg agatgtatct
     3001 gcgcttttaa gagagtggcg caccatgaaa acgagtgagc acttcccgcc ctgtattcag
     3061 cgaaatatgg tggagaaggc cttgaataag gctcaggtgg tcggattgac aaagccactg
     3121 gatcagaaaa tttatgcgtt gagctatctg aatggaaaga aagcgtatct tgagtcagcc
     3181 gaatttcagg tcatgctgga aaaggtgaaa cgaaatgaaa tatcgctgtc tgaccttggt
     3241 cgccagtgac atacatcttt gccaaccaat tgataaaagg gaataaaatc tgatggggct
     3301 atttacccga ctggaaaaat ttgaccaaaa gcttacgcgt ggttatgccc gctgggggcg
     3361 ttgggtgtgg cggatgctaa tagcgatccc cgtggcttat tttttactgt gtgtaggaat
     3421 atcaatctgg ggagctccaa ctggaggagt catattggtg atccatagtg aactggatag
     3481 gccaatatta ggattcagcg tgaacggcat ggctggggca aatgcgtttg cacatggtgg
     3541 aggttcaacg acttgctgcg gtgacatcag agggaaagaa gctgaggtcg tctggacatt
     3601 gagtacgact cgcgctcagt ataatacggg actgcgtgaa gaagttcgac gtatcactct
     3661 gcctttgcct gaaaggaaac atgggcagga ttttttgcat gtacatttct tgcctggtga
     3721 taaggttttt ttgggttgga gtgaaggagc tggttcgccc tatgagaaac gaaaagaacc
     3781 ttcttatccg agtcgaaaaa atcaggaggc gcagccgtga taagtgaatc agtgatttcc
     3841 gcagctgtac gagcacaaca gtcagaagat ggtaaagtgg gaaattgtgg ccggatctgg
     3901 catgtcgctt tcttttttga tggagtggga cgaaatatcg accgggatgc gacagactgc
     3961 cgtttgagta atattgcccg cctgttccgg gcatatcctg atgaacaaaa aaatacaggt
     4021 gatacttgtt ataataaatt ttatttctcc ggaatgggca ctgcctacca tgacgattct
     4081 gtcgacaata ttcacgctct tatggatatt ggtttagata actttctgga ggatatgaaa
     4141 agtctgccag aggatactat atcggatgca ggggagtctg ttattaaagg agaaaagaaa
     4201 tggacggatg tgcttggtaa tctccttgag gatctcaata atccggtaga gtgggttaaa
     4261 gggattggca agatggcggc taagtcggta ggcaaagcag gaattgagtc cacaccttgg
     4321 ttgcgtgata gcgaagccat gagcgcctat tttatgacgg gagaacccgt acgccttgaa
     4381 gctgcaaagc gattgtttaa gaagttttat acggaaaata tgaagggaga tgttcccatc
     4441 aaaaaaattt ctgtttcatt atacggtttt gaccttggag ctacactggc acgtaaattt
     4501 ttggataatt ttcttaagga agtttgcctg aaaaataaag aaaaaaaaca ccaatataaa
     4561 aacgcgttgg tagatattgc ttttattggt ctttttgact gttctcggca ttcaccgact
     4621 agtagtaata atgggttgga ctattttttt atgtggttgg gtttaccagg gaaaatgatt
     4681 ggttcagtat taggtgaaaa agctattgat caggactcac cactgccgga cgtagtaaat
     4741 aacgcacttc accttgtagc agctcatgaa cggcgtccat ggcgtggtct gtatctgttg
     4801 gggaaaaaaa cggatacagc gaataaaaaa tacagggaag aactgcttcc cggttgtagt
     4861 gaagatattg gtggtggctt gaaaccggat gaacagaaac ccagtgcaga actgtgcagg
     4921 gttgcactat acaatatgta tcatgcagca tgtcaagctg gtgtgccatt tcctgatttt
     4981 gacactttgg atcaatatga tacgaaagtt gccagctatt ttattatgaa tgatgctgtt
     5041 cagggaggat ctgtaaatta ctgggttagt cgttatcaaa gagaggaagt gaaggataaa
     5101 gtatttagtg aggcaatgca ggagaagtat ctagataact attttctctg gttgggtgag
     5161 caatattata tgtatcagta tgaacgggaa cgtctggata aggagttgac atcggctcat
     5221 cgtgggaaaa taagtgacta tgggccactt gcaggtacgg gaattaatcc aaatactaac
     5281 gctgatgata taaaatcgca gatttcagaa cttgactcac tatggggatg gttgaatgat
     5341 gtaaaaagtg tagctattgg attacgaaat gactttatag tcaacccgag gaaaaatgat
     5401 agccgaataa aacttaaacc tcaggtttat aatgctgctg tcaatcgggc tgtgaaattc
     5461 cttgagtttt ctcgcgcagt ttataatgat aaaaatctgc catcatcatc atctttgacg
     5521 gcaaatacac tatattcctt ttttgtgcat gatatacaaa aggttgatca cacaccttct
     5581 atatctgaag atttcttctt acgtcgctca tctgaaattc cagatgcgga tgaacctcca
     5641 ggaaataaag atgatggaaa gaaacatact c
//
LOCUS       DAHHTG010000205         5535 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.209, whole genome shotgun sequence.
ACCESSION   DAHHTG010000205 DAHHTG010000000
VERSION     DAHHTG010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5535)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5535)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5535
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.209"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            48..389
                     /locus_tag="NBI74_004331"
     CDS             48..389
                     /locus_tag="NBI74_004331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1934561.1"
                     /translation="MKTSKTVAKLLFVVGALVYLVGLWISCPLLSGKGYFLGVLMTAT
                     FGNYAYLRAEKLGQLDDFFIHVCQLVALITIGLLFIGVLNAPINAYEMVIYPIAFFVC
                     LFGQMRLFRSA"
     gene            435..872
                     /locus_tag="NBI74_004332"
     CDS             435..872
                     /locus_tag="NBI74_004332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiaB family inner membrane protein"
                     /protein_id="HCD1934562.1"
                     /translation="MDNKISTYSPAFSIVSWVALIGGIVTYLLGLWNAEMQLNEKGYY
                     FAVLVLGLFSAASYQKTVRDKYEGIPTTPIYYMTCLTVFIISVALLLVGLWNATLLLS
                     EKGFYGLAFFLSLFGAVAVQKNIRDAGINPPKETQVTQEEYSE"
     gene            complement(914..1909)
                     /gene="wecH"
                     /locus_tag="NBI74_004333"
     CDS             complement(914..1909)
                     /gene="wecH"
                     /locus_tag="NBI74_004333"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyltransferase WecH"
                     /protein_id="HCD1934563.1"
                     /translation="MQPKIYWIDNLRGIACLMVVMIHTTTWYVINAHSVSPITWDIAN
                     VLNSASRVSVPLFFMISGYLFFGERSAQSRHFLRIGLCLFFYSAIALLYIALFTSINV
                     ELALKNLLQKPVFYHLWFFFAIAVIYLVSPLIQVKNVGGKMLLVLMVVIGIIANPNTV
                     PQKIDGFEWLPINLYINGDTFYYILYGMLGRAIGMMDTQHKALSWVSAALFATGVFII
                     SRGTLYELQWRGNFADTWYLYCGPMVFICAIALLTLVKNTLYTRTICGLGLISRHSLG
                     IYGFHALIIHALRTRGIELKNWPILDIIWIFCATLAVSLLLSMLVQRIDRNRLVS"
     gene            2084..2383
                     /locus_tag="NBI74_004334"
     CDS             2084..2383
                     /locus_tag="NBI74_004334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YsaB family lipoprotein"
                     /protein_id="HCD1934564.1"
                     /translation="MMMNAFFPAMALIVLVGCSTPPPVQKAQRVKVDPLRSLNMEALC
                     KDQAAKRYNTGEQKIDVTAFEQFQGSYEMRGYTFRKEQFVCSFDADGHFLHLSMR"
     gene            2478..3389
                     /gene="glyQ"
                     /locus_tag="NBI74_004335"
     CDS             2478..3389
                     /gene="glyQ"
                     /locus_tag="NBI74_004335"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HCD1934565.1"
                     /translation="MQKFDTRTFQGLILTLQDYWARQGCTIVQPLDMEVGAGTSHPMT
                     CLRALGPEPMAAAYVQPSRRPTDGRYGENPNRLQHYYQFQVVIKPSPDNIQELYLGSL
                     KELGMDPTIHDIRFVEDNWENPTLGAWGLGWEVWLNGMEVTQFTYFQQVGGLECKPVT
                     GEITYGLERLAMYIQGVDSVYDLVWSDGPLGKTTYGDVFHQNEVEQSTYNFEYADVDF
                     LFTCFEQYEKEAQQLLALENPLPLPAYERILKAAHSFNLLDARKAISVTERQRYILRI
                     RTLTKAVAEAYYASREALGFPMCNKDK"
     gene            3399..5468
                     /gene="glyS"
                     /locus_tag="NBI74_004336"
     CDS             3399..5468
                     /gene="glyS"
                     /locus_tag="NBI74_004336"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HCD1934566.1"
                     /translation="MSEKTFLVEIGTEELPPKALRSLAESFAANFTAELDNAGLAHGT
                     VQWFAAPRRLALKVANLAEAQPDREIEKRGPAIAQAFDAEGKPSKAAEGWARGCGITV
                     DQAERLTTDKGEWLLYRAHVKGESTEALLPNMVATSLAKLPIPKLMRWGASDVHFVRP
                     VHTVTLLLGDKVIPATILGIQSDRVIRGHRFMGEPEFTIDNADQYPEILRERGKVIAD
                     YEERKAKIKADAEEAARKIGGNADLSESLLEEVASLVEWPVVLTAKFEEKFLAVPSEA
                     LVYTMKGDQKYFPVYANDGKLLPNFIFVANIESKDPQQIISGNEKVVRPRLADAEFFF
                     NTDRKKRLEDNLPRLQTVLFQQQLGTLRDKTDRIQALAGWIAEQIGADVNHATRAGLL
                     SKCDLMTNMVFEFTDTQGVMGMHYARHDGEAEDVAVALNEQYQPRFAGDDLPSNPVAC
                     ALAIADKMDTLAGIFGIGQHPKGDKDPFALRRAALGVLRIIVEKNLNLDLQTLTEEAV
                     RLYGDKLTNANVVDDVIDFMLGRFRAWYQDEGYTVDTIQAVLARRPTRPADFDARMKA
                     VSHFRTLEAAAALAAANKRVSNILAKSDEVLSDRVNASTLKEPEEIKLAMQVVVLRDK
                     LEPYFAEGRYQDALVELAELREPVDAFFDKVMVMVDDKELRINRLTMLEKLRELFLRV
                     ADISLLQ"
BASE COUNT         1404 a         1349 c         1426 g         1356 t
ORIGIN      
        1 tttcgttggt tactcgtaat gtatcgctgg tagatatgga gatcgttatg aaaacctcaa
       61 agactgtggc aaaactatta tttgttgtcg gggcgctggt ttatctggtt gggctatgga
      121 tctcatgccc attgttaagt ggaaaaggct attttcttgg cgtgttaatg acggcaactt
      181 ttggcaacta tgcatatctt cgcgcagaaa aacttgggca actggatgat ttttttatcc
      241 acgtctgtca gttggtagcg ttaatcacta tcggtctctt gtttatcggt gttttaaacg
      301 cacctatcaa tgcttatgaa atggtgatct atcccatcgc cttttttgtc tgcttgtttg
      361 gtcaaatgcg tttgtttcgc tcggcatgag caacataaag ttcttacaca ttcaggaatg
      421 aaaggaatac tgtgatggac aacaaaatat caacctattc tccggcattt agtattgttt
      481 catgggtagc ccttattggc ggtattgtta cctatctgtt aggactatgg aatgcagaga
      541 tgcagttaaa tgaaaaaggg tattattttg ccgtactggt attaggtttg ttttccgcgg
      601 cgtcttatca aaagaccgtt cgggacaagt atgaaggcat acctaccacc cccatttatt
      661 atatgacttg cctgacggtc tttattatct ctgttgcgtt gttgctggta ggtctatgga
      721 atgcaacgtt attactcagc gaaaaaggct tttatgggct ggctttcttc ttaagcttgt
      781 ttggtgcagt agcggtgcag aagaatattc gtgatgccgg aataaaccca ccaaaagaaa
      841 cacaggttac ccaggaagaa tacagcgaat aattcacgta agcccggtca gtccaatgtg
      901 accgggcttc tacttaactc actaatctgt ttctgtcgat tcgttgtacc agcatagaaa
      961 gtaataaact cactgccagc gtcgcacaaa agatccaaat aatatccagt attggccagt
     1021 ttttaagctc aatcccccgg gtgcgcagtg catggataat caatgcatgg aacccgtata
     1081 tacctaacga atggcgggag attaagccaa gtccgcaaat ggtacgcgta tacagcgtgt
     1141 ttttaaccag agtcaataac gcgattgcgc agataaaaac catcggccca cagtaaagat
     1201 accaggtatc ggcaaaattt ccgcgccact gtaattcata taatgtcccg cgagagataa
     1261 taaaaacccc cgtcgcaaac agtgcggcgc tcacccacga cagtgcttta tgctgtgtgt
     1321 ccatcatccc tatagcgcgg cccaacatgc cataaaggat gtagtaaaaa gtatcgccat
     1381 tgatatataa gttaattggc agccattcaa aaccgtcgat tttctgcggc acggtgtttg
     1441 ggttagcgat gataccaatc accaccatta gcaccagcaa catttttccg ccgacgttct
     1501 tcacctgaat cagcggtgaa accagataaa tcactgcaat cgcgaagaaa aaccataaat
     1561 ggtaaaacac tggcttttgc agcaggtttt tcagcgctaa ctccacattg atggaggtaa
     1621 acagcgcaat gtagagcagc gcgattgcgc tataaaaaaa caggcataag ccgatacgca
     1681 agaaatgacg cgactgggcg ctgcgttcgc caaaaaagag atagccggaa atcatgaaaa
     1741 atagcggcac gctgacacga gaggcagaat tcagaacatt ggcgatatcc caggtgatgg
     1801 ggctaacact atgagcattg atcacatacc aggtagtggt gtgaatcatc accaccatta
     1861 aacacgctat ccctcgcagg ttatcaatcc agtaaatttt gggctgcatc tgtgtctctg
     1921 tatctggtta aaaaaagtct gaccgataaa tcattcagtt ggcgcactgg aataatctga
     1981 gttttatcac tacagcttat agaggcttaa ggaaattcgt aagatatcag ccactatacc
     2041 gatataaata ataagactca cctgcaaacc agacggtaat ttaatgatga tgaacgcttt
     2101 ctttccggca atggcgctta ttgtgctagt gggttgttct acaccgccac ccgtgcagaa
     2161 agcacaacgg gtaaaggttg atcctctgcg ttcgttgaat atggaagcgt tatgcaagga
     2221 tcaggcggca aaacgttata acaccggcga gcaaaaaatc gacgtcaccg ccttcgaaca
     2281 gttccaggga agctatgaaa tgcgcggtta taccttccgg aaagagcagt ttgtctgttc
     2341 ttttgacgcg gatggccatt ttttgcatct ttccatgcgt taagccctgc tttttcccgt
     2401 ttcgtactgt atatcttcca tccagcgggt atactgatcc cttcctttaa atccacacgt
     2461 atccagcacg aaataatatg caaaagtttg ataccaggac cttccagggc ttgatcctga
     2521 ccttacagga ttactgggct cgccagggct gcaccattgt tcaaccattg gacatggaag
     2581 tcggcgcggg aacctctcac ccaatgacct gtctgcgcgc gctggggcca gaaccgatgg
     2641 cggctgctta tgttcagcct tctcgtcgcc cgaccgatgg tcgctacggc gaaaacccca
     2701 accgtttaca gcactactat cagttccagg tggtcattaa gccatcgccg gacaatattc
     2761 aggagctgta cctcggttct ctgaaagagc tgggcatgga cccgaccatc cacgacatcc
     2821 gtttcgtgga agataactgg gaaaacccga cgctgggtgc ctgggggctg ggctgggaag
     2881 tgtggctaaa cggcatggaa gtgacgcagt tcacttactt ccagcaggtt ggtggtctgg
     2941 aatgtaaacc ggttaccggt gagatcacct acggtctgga acgtctggcc atgtacattc
     3001 agggcgtaga cagcgtttac gacctggtct ggagcgacgg cccgctgggt aaaaccacct
     3061 acggcgacgt gttccatcag aacgaagtgg agcagtccac ttacaacttc gaatacgcgg
     3121 atgtggactt cctgttcacc tgcttcgagc agtacgagaa agaagcgcag caactgctgg
     3181 cgctggaaaa tccgctgccg ctgccagcct acgagcgtat tctgaaagcc gcccacagct
     3241 tcaacctgct ggatgcgcgt aaagccatct ccgtcaccga gcgtcagcgc tatattctgc
     3301 gcattcgcac cctgaccaaa gcagtggcag aagcatacta cgcttcccgt gaagccctcg
     3361 gcttcccgat gtgcaacaaa gataagtaag aggcggctat gtctgagaaa acttttctgg
     3421 tggaaatcgg cactgaagag ctgccaccaa aagcactacg cagcctggct gagtcctttg
     3481 ctgcgaactt tactgcggag ctggataacg ctggcctcgc acacggcacc gttcaatggt
     3541 ttgctgctcc gcgtcgtctg gcgctgaaag tcgctaacct ggcggaggcg caaccggatc
     3601 gtgaaatcga aaaacgtggt ccggcaattg ctcaggcgtt cgacgctgaa ggcaaaccga
     3661 gtaaagcggc agaaggttgg gcgcgtggtt gcggtattac cgttgaccag gctgagcgtc
     3721 tgactaccga taaaggcgaa tggctgctgt atcgcgccca tgtgaagggc gaaagcaccg
     3781 aagcactgct gccgaatatg gttgcgactt cgctggcgaa actgccgatc ccgaaactga
     3841 tgcgttgggg cgcaagcgac gtgcactttg tgcgtccggt tcacaccgtg accctgctgc
     3901 tgggcgacaa agttattccg gctaccattc tgggtattca gtccgatcgc gtgattcgcg
     3961 gccaccgctt tatgggcgag ccggaattca ctatcgacaa tgccgatcag tatccagaaa
     4021 ttctgcgtga gcgcgggaaa gtcatcgccg attacgaaga acgtaaggcg aagattaaag
     4081 ccgatgctga agaagcagca cgtaagattg gcggtaacgc tgatttaagc gaaagcctgc
     4141 tggaagaagt ggcttcgctg gtggagtggc cggtcgttct gaccgcgaaa ttcgaagaga
     4201 aattcctcgc ggtgccgtct gaagcactgg tttacaccat gaaaggtgac cagaagtact
     4261 tcccggtgta tgcgaacgac ggcaaactgc tgccgaactt tatcttcgtt gccaacatcg
     4321 aatcgaaaga tccgcagcag attatctccg gtaacgagaa agtcgttcgt ccacgtttgg
     4381 cggatgccga gttcttcttc aataccgacc gtaaaaaacg tcttgaagat aacctgccgc
     4441 gcctgcaaac cgtgttgttc cagcaacagt tggggacgct gcgcgacaaa actgaccgca
     4501 ttcaggcgct ggccggctgg attgctgaac agattggcgc tgacgttaac cacgctaccc
     4561 gtgcgggcct gctgtctaag tgtgacctga tgaccaacat ggtcttcgag ttcaccgaca
     4621 cccagggcgt tatggggatg cactacgcgc gtcacgatgg cgaagcggaa gacgtagctg
     4681 tcgcgctgaa tgagcagtat cagccgcgct ttgccggtga tgacctgccg tctaacccgg
     4741 tagcctgtgc gctggcgatt gctgacaaga tggataccct ggcgggtatc ttcggtatcg
     4801 gccagcatcc gaaaggcgac aaagacccgt ttgcgctgcg tcgtgccgca cttggcgtgc
     4861 tgcgtattat cgttgagaag aacctcaacc ttgatctgca aacgctgacc gaagaagcag
     4921 tgcgtctgta tggcgataag ctgactaatg ccaacgtggt tgatgatgtt atcgacttta
     4981 tgctcggtcg cttccgcgcc tggtatcagg acgaaggtta caccgttgac accatccagg
     5041 cggtactggc gcgtcgtccg actcgtccgg ctgatttcga tgcccgtatg aaagcggtat
     5101 cgcacttccg taccctggaa gcagctgcag cactggcggc ggcgaacaag cgtgtatcta
     5161 acatcctggc gaaatctgat gaagtgctga gcgatcgcgt gaatgcctct actctgaaag
     5221 agccggaaga aattaaactg gcgatgcagg ttgtggtgct acgtgacaag ctggagccgt
     5281 actttgcgga aggtcgttac caggatgcgc tggtcgaact ggctgagctg cgtgaaccgg
     5341 tcgatgcttt cttcgataaa gtgatggtca tggttgatga taaagaattg cgtatcaacc
     5401 gtctgaccat gctggaaaaa ctgcgcgaac tgttcctgcg cgttgcggat atttcgctgt
     5461 tgcagtaata acgccgttat taaaaagcct gccatctggc aggctttttt tatcgctaaa
     5521 taatacagca acctt
//
LOCUS       DAHHTG010000206         5367 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.210, whole genome shotgun sequence.
ACCESSION   DAHHTG010000206 DAHHTG010000000
VERSION     DAHHTG010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5367)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5367)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5367
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.210"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            complement(1..716)
                     /locus_tag="NBI74_004337"
                     /pseudo
     CDS             complement(1..716)
                     /locus_tag="NBI74_004337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     misc_feature    complement(319..435)
                     /locus_tag="NBI74_004337"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01497"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="AL1L pseudoknot; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /pseudo
                     /db_xref="RFAM:RF01497"
     gene            complement(807..920)
                     /locus_tag="NBI74_004338"
     CDS             complement(807..920)
                     /locus_tag="NBI74_004338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001513409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HCD1934567.1"
                     /translation="MKMKHLRDDVAAGRRRLYIRAICPSLREAPLLYSYPL"
     gene            complement(1456..2205)
                     /locus_tag="NBI74_004339"
                     /pseudo
     CDS             complement(1456..2205)
                     /locus_tag="NBI74_004339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343760.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            2754..3014
                     /locus_tag="NBI74_004340"
     CDS             2754..3014
                     /locus_tag="NBI74_004340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001110186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HCD1934568.1"
                     /translation="MNYVICTTIVALGLILTGYATAADEINRQDVVGLMPSDYISVGN
                     LYTPEEVATALKDKAEQAGGSHFRITSLGGKNKLDGTAIIYR"
     gene            3056..3616
                     /locus_tag="NBI74_004341"
     CDS             3056..3616
                     /locus_tag="NBI74_004341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000109148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCD1934569.1"
                     /translation="MSTYENILRITDTLIQQRGFLGFSYADLEKEIGIRKASIHHHFP
                     RKTDLGIAYCQYKTEVFDRLNITLHNVSSGVQRLRTYLDAFAGCAERGEMCGIYAMLS
                     DSHQFSPELQEAVSQLAHQEIQMIKDIITSGQNSGEFKTVLLPDELAVIVCSTLKGAL
                     MLNRLPPHDIYSGTVNALIKMLDTRT"
     gene            3656..4084
                     /locus_tag="NBI74_004342"
     CDS             3656..4084
                     /locus_tag="NBI74_004342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF417 family protein"
                     /protein_id="HCD1934570.1"
                     /translation="MKKFINVYIKSDYDIVILRLSVIIIFMLFGTYKWFDFEVMALKP
                     LISPTWLNILYIMFGVHGGSYFLGIVETITFLALSIGFFRPAAGILGDIIVIVTGLVT
                     LSLLPQLGKVDSFVIKDVLLLGAGSVLLKYDLKNYLNIKN"
     gene            complement(4227..4440)
                     /locus_tag="NBI74_004343"
                     /pseudo
     CDS             complement(4227..4440)
                     /locus_tag="NBI74_004343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001444904.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helicase"
     gene            4748..5367
                     /locus_tag="NBI74_004344"
                     /pseudo
     CDS             4748..5367
                     /locus_tag="NBI74_004344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611435.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         1406 a         1310 c         1177 g         1474 t
ORIGIN      
        1 ctggtcagga cgttctgcca cgaactgacg gtttacgcgg tcacctgcag caacggtttt
       61 ccggctgacg gtagtgcgga cctttttacc ccggagaaca ccggcaagtc ccataaccgc
      121 catgaggcgc gccactgtac atctggccac cctgattcct tcccgtaaca actgacgcca
      181 gactttacgc acaccgtaca cctgatgatt ttcatcgtat acgcgctgta tctctctctt
      241 cagccagtca tcgcgctgac aacgggcact gcgtttatca ggatgatgcc gctgctgctg
      301 acaatggtaa tacgttgacg gggcaatatg ctgttcactg cataccggtc cgaccccgta
      361 ctgctcacgc agcttatcca gcagtggcat tattttttcc agaggcggtc gaactccgcc
      421 ttcgcaaaat aagcggaagc ctggcgaagg atatcgttac tgcggcgcag ttcacgattt
      481 tcacgttcca gctctttcag acgctgacgt tcagcggtgg tgagcccacc atcaccgccc
      541 ccggtatccc gctcatgctg gcgaacccag acacgcagag tctctggcgt acagccaatc
      601 tttggagcta tggaacaaat tgccgcccat tgtgagtcat attcgtcctg actttccaga
      661 accatacgaa ctgcccgttg acggacttcg ggggaaaaac gtgtattttt agtcatcctg
      721 tttacctctt tctcagggaa tttagtctcc aggattcccg gggcggttca cagtaatcat
      781 gccgggtctg gctggctaac accccattat aaggggtacg aataaagtaa cggggcttca
      841 cgcagggatg gacagattgc tctgatatac agccgacgac gtcccgctgc aacatcatca
      901 cgcagatgct tcattttcag atttgggacc tccttttgat ttctcgttta acttttgctg
      961 ttaccagact ttaatctatt ttaacaattc aagaattttt tataaaatgc tcaaagatga
     1021 aagcgttact gaaccaacca gcttggctga tgttttctat agataaaaag atcatcagca
     1081 aaaactgata gcctgaatca cctttactac caatcatgta tgaagaaaac acgcacaaac
     1141 aattgacaat gattatcatt tgcattaaaa ggattatgta gtatttttat ttcatgagga
     1201 actatacccg ccagcaatct gagtagcgaa agctgttcat ccccaatatt tttgctggcg
     1261 ggttcttttt tctactgccc ttctcctcag agggagcatt ccattctttt tttcattaat
     1321 ggcgaggggg cgggacaaaa agccggttct ttatccctaa ataacagcat gatttcagtc
     1381 catgcaaaat ctgatttacc agctcacata ataacctgat gatacatggg ttttccctga
     1441 tcatggtggg aaggctcaga acgccatatt cagtttcccg tagtggaaca tcacgcccag
     1501 cttaaagcgc tcccgatttc ggtatcccct agcttttatc ctcagtagcc tgatcttact
     1561 gttaagcgcc tccgcgtttc cgtttgagac acggtgtcgc atggcattca ggatcccgta
     1621 aagccttttt cctatcgttt tcgcggcgtt tttcatcata ggaacgtcac tgttggccgc
     1681 cagagccagc catctctccc agtcattacg tctttcctca ctccatggcc tgttccagat
     1741 atcctttgcc agctctttca gcgcccagca ctggcttgtc agcttcatct gtgcacgcag
     1801 ccatatcagc ttttcctgcc tggatttggt catccattta tcgctgtact gccacaggaa
     1861 gcgggtcccc tttgcctgac gccaactttc aacagggagg tgcggatgtt cattctgacg
     1921 ggttttatca accacttcgc caagttgctt tgctacatgg aagcggtcga aggcgatttt
     1981 ctcaaccgca cagggtaagt ggatacgcgc tgctcttata tagcctgcat tcatgtccat
     2041 tgagagcgtt ttgatagcca gcaactgccc gtcagtaagc gtgcgaagat agccggcgag
     2101 gctctctgtg ccacgatcgt ccgttaaggc cagcgcacga ccatcgcgat cggagatcac
     2161 cgttatataa cgatgtcctt ttttaaaggc gacctcatcc acattgtcag caccagtgat
     2221 ataagacgga tagcaaagcg cacgcgctgt ttgtcaccgt gaaaaacgac ggtgcgacgg
     2281 caaaagccaa taacaacgag agcgtcgtgc gcattcctct gcactgacag ttgattctta
     2341 gaacgtagag gcctggattg ctgccaggct tttattgcac taccagctac ggctaaaaga
     2401 taagcgtccc gggaagtaca tagcgcttac aaaatatccg acatgaagca tactggaaac
     2461 gaggcgtatt gttattgttg ttcatattgg cttccgtggc ggaaatatca gggggtagct
     2521 acatatcccc catgcagcca aggcaacgat gttaactctc gtcacctgtt cctttcgaca
     2581 gagccaacct cgcagagggc cagatcttac aacttcacct cagtgataca aaacacaaaa
     2641 atacaaaaca atttgataca aatcatccct cttattttaa ataaatccat cttgcaagcc
     2701 taccaatcag aaggtagcat gtgtagtatc gcaaacataa agagaaattg ctcatgaatt
     2761 atgttatctg cactaccatt gtcgcccttg gtctcattct gacaggctac gcaactgctg
     2821 cagatgaaat taatcgtcag gacgttgttg gtttgatgcc gtcagactat atttctgttg
     2881 gtaatctata tacaccagaa gaggtggcaa ctgcgcttaa agacaaagcg gagcaggcag
     2941 gaggttctca tttccgaatc acttcattgg gaggaaaaaa taaacttgat ggaactgcca
     3001 ttatctatcg ctaaaatatg tataatagtc cctttaatac ataacaggta aagccgtgtc
     3061 tacttatgag aatatacttc gcataacgga tactctgatc cagcaacgcg gatttttggg
     3121 gttcagttac gcagatctgg aaaaagaaat tggtatcaga aaagccagta ttcatcacca
     3181 tttcccgagg aaaactgatc ttggtatagc ctactgccaa tacaaaacag aagtatttga
     3241 tagacttaat ataactctcc ataatgtttc ttcaggtgta caacgtctca ggacatatct
     3301 tgatgcattt gctggttgtg ctgaaagagg ggaaatgtgc ggtatttatg ccatgctatc
     3361 cgacagccat cagttttctc ctgaattaca ggaggctgta agtcaactgg cacatcagga
     3421 gatacagatg attaaagata ttatcacctc cgggcagaat agtggagagt tcaaaacagt
     3481 tcttcttccg gatgagctcg ccgttatagt ctgttctaca ctaaaggggg cactgatgtt
     3541 aaatcgactt ccaccacatg atatctattc aggaaccgtt aacgctttaa ttaaaatgct
     3601 tgatacccgt acttaatcct ttttagtgca tataattttc cacccggaga atgaaatgaa
     3661 aaaatttatt aatgtttaca ttaaatcaga ttatgacata gttattcttc gcctgtctgt
     3721 tattataata tttatgctct tcgggaccta taaatggttt gattttgaag tgatggccct
     3781 caagcccctg atttctccca cgtggctgaa tatcctgtac attatgtttg gtgttcatgg
     3841 ggggagctat ttcctcggca tagtagaaac tatcactttt ctagcactga gtatcggctt
     3901 cttcaggcct gctgctggca tactgggaga tattatcgtt attgttacag gtttagtcac
     3961 actaagtctg ttaccacaac tgggaaaagt tgacagtttt gtcatcaaag atgtgctgct
     4021 gttaggtgct ggtagtgtcc tgctgaaata tgatttgaaa aattatttaa atatcaaaaa
     4081 ctgatttttc acttcggtga tgaacgtttg cgtattttca tggaaagaga ttacctaatc
     4141 cccctcgttc tccttcatgt taatacgcaa cgttatggaa aacaaaaata ccctgagtaa
     4201 aaaggtccgt aggcaggcga gtacttttat tttccgctag ttttactttc ccatcgaagg
     4261 ccccatacat cactacgccc acgaaacaac cgacgaaata acgcaacttt ctcatccgtg
     4321 gataatacgg aaacgcactg cacaggactc tgcggtttac gtcgccattc aatcccataa
     4381 gcttcaagtg atgtcttccc tcagtgctgc taattcatca ctgtcagatt tatgaaccat
     4441 attcgttacc tgtatccgaa ccactgaagc accacagcag aaattcgggt cccctacact
     4501 gattacagcg ccccctctgc tgcctattaa ctcaagcacc tcccaacaac ttacggaaac
     4561 ttaattctaa tacagctgta attcagttgt gtcgtggtcg gccgcggtta cttttttcgg
     4621 gaagtctgct acgcatcaga ataataccgt gttctttctg atcctgttta gccaggtagc
     4681 taagggcgta acgtaaaccg ttagactggc cccctgaatc tccagacaac caatatcact
     4741 taaataagtg atagtcttaa tactagtttt tagactagtc attggagaac agatgattga
     4801 tgtcttaggg ccggagaaac gcagacggcg tactacacag gaaaagatcg ctatcgttca
     4861 gcagagcttt gaaccgggaa tgacggtctc ccttgttgcc cggcaacatg gtgtggcagc
     4921 cagccagcta tttctctggc gtaagcaata ccaggaggga agtcttactg ctgtggctgc
     4981 cggagaacag gtcgttcctg cctctgaact tgctgccgcc atgaagcaga ttaaagaact
     5041 ccagcgtctg ctcggcaaaa aaacgatgga aaatgaactc cttaaagaag ccgttgaata
     5101 tgggcgagca aaaaagtgga tagcgcacgc gcccttattg cccggggatg gggagtaagc
     5161 ttcgtcagcc gttgtctccg ggtgtcgcgt gcgcagttgc acgtcattct cagacgagcc
     5221 gatgactgga aggacggccg ccgcagccgt cacacggatg atacggatgt gcttcgccgt
     5281 atacaccatg ttatcggaga gctacccaca tatggttatc gtcgggtatg ggcgctgctt
     5341 cgcagacaaa cagaacttga tggtatg
//
LOCUS       DAHHTG010000207         5332 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.211, whole genome shotgun sequence.
ACCESSION   DAHHTG010000207 DAHHTG010000000
VERSION     DAHHTG010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5332)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5332)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5332
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.211"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            complement(81..1457)
                     /locus_tag="NBI74_004345"
     CDS             complement(81..1457)
                     /locus_tag="NBI74_004345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001683247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system ImpA family N-terminal
                     domain-containing protein"
                     /protein_id="HCD1934571.1"
                     /translation="MASNANFISQFVMGGDPCTYKESGELQAEMSKLTHPARPDVDWR
                     QVEKLCLALFRQNGVELQTLVCYVLAITRRQGLAGMADGLGSLDILLQRWADFWPVQV
                     HSRISLLSWVTEKMQQALRTLDIQYQDLPQIYRCVQHLSAIETTLQQCELWHMTKLDV
                     LAGQFRNTALRLERLAPQGAETTITPPELPRREMNQPKKSEESPQPVFATRPAQQNDK
                     DASPPVSSPEISRQRAWPIFMAGMVVMACLGGTGLWVWSQLNQPDALIQRIQLSIMPL
                     PQSLESGELAKLDVKDKALLAQDRTIAASQMQLEQLNKLPARWPLEQGYRQLRQLDAL
                     WPDNPQVRALNAQWRKQRELSALSAEALNGYAQAQSQLQRLSAQLDALDERKGRYLTG
                     SELKTAVYGIRQSLKEPPLEELLRQLEEQKQTGEISPTLLTQIDTRLNQLLNRYVILL
                     DTKVEQSQ"
     gene            complement(1457..1885)
                     /gene="tssE"
                     /locus_tag="NBI74_004346"
     CDS             complement(1457..1885)
                     /gene="tssE"
                     /locus_tag="NBI74_004346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000106970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssE"
                     /protein_id="HCD1934572.1"
                     /translation="MPTPSLYEMLTFSFSGELPLEQVRERDQLILSVMDNMQRIINCR
                     AGTLAHLPDYGLPDLSLIHQGMAAGIHGLMRQIEETLLRYEPRLSQIQVELLPQPRPG
                     HLNYLIHAQLPDTGWIRFDGVFSPEGRIVLRHLKQQERAY"
     gene            complement(1889..2425)
                     /gene="tssJ"
                     /locus_tag="NBI74_004347"
     CDS             complement(1889..2425)
                     /gene="tssJ"
                     /locus_tag="NBI74_004347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000484007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system lipoprotein TssJ"
                     /protein_id="HCD1934573.1"
                     /translation="MFPIRFKRPALLCMAMLTVVLSGCGLIQKVVDESKSVASAVFYK
                     QIKILHLDFFSRSALNTDAEDTPLSTMVHVWQLKTREDFDKADYDTLFMQEEKTLEKN
                     VLAKHTVWVKPEGTASLNVPLDKETQFVAIIGQFYHPDEKSDSWRLVIKRDELEADKP
                     RSIELMRSDLRLLPLKDK"
     gene            complement(2406..3485)
                     /gene="tssG"
                     /locus_tag="NBI74_004348"
     CDS             complement(2406..3485)
                     /gene="tssG"
                     /locus_tag="NBI74_004348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000373314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssG"
                     /protein_id="HCD1934574.1"
                     /translation="MDRASQPARTGLTDRLAPDIARINFYRFCQLLEQRQQNAPLGST
                     DSPAADAVRFRPHPGMGFPVSELKNVERDVDNPDAPPTVRTTFLGLYGVDSPLPTAYL
                     DYITQRHDGHDAVMAFLDIFNHRFITQYYRIWRKYNYPASFEAGAVDDISRCLLGLIG
                     LGIPGSENHIATPVSRFLALLSVMRLPTRTAEGVTALVGLLAPLTKATVVPHDPQPVI
                     LPAPAGLSKNSPISLKTRTLLGRTGTDVNSQLLLKLYTEDAAEARGWLPGGQLHSDLL
                     VLLRVYLGWRCQARLQLTLPVSLLPAARLGKQRVQISRTGILRASFAAPATGTVTVSL
                     GRYQGLTPAFSIRNRESMTHVSYSF"
     gene            complement(3449..5209)
                     /gene="tssF"
                     /locus_tag="NBI74_004349"
     CDS             complement(3449..5209)
                     /gene="tssF"
                     /locus_tag="NBI74_004349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssF"
                     /protein_id="HCD1934575.1"
                     /translation="MDDLTQRYFEAEMRYLREAGKEFAQAYPDRAAMLNLDKPGARDP
                     YVERLFEGFAFLMGRLREKLDDDLPELTEGLVSLLWPHYLRTIPSLSVVELTTDHQQM
                     KQSDTLKEFQVLSRPIGERRTRCVYSATRDITLHPLALPDVSLQYEPDGRSVIRLRFE
                     CGPLVGDWSQIDLSRLPLYLSADSPVACALHRALTLGTQQFWLRLPGQGRRVLDAHFS
                     PMGFDDDDRLWPKGESAFSGYQLLLEYFTFREKFMFVALNGLEQVAWPEGITGFEIDV
                     LLNENWPHDLPFDSDNIRLHCVPVINLFPLEADPLHLSPLENEFLLRPMRIQDGHTEI
                     YSVDNIISSRHTGSQAYVPFSSFRHRGGMLRHDAPERYYHTRVKRGPSGLHDTWLILG
                     GDAFDTDRMLEDETLSLSLTGTNGQLPRKALQSTLLDTPVHASQNVLRVRNLCAPTQP
                     CYPPARDRFHWRVLSHLGSNFLSMMDNAEILRGTLALYDWTESEMNRRRLAAIVDVQH
                     SLIQRFERGFLLRGVDIQVTLDSNGFAGEGDITLFGELLHRFFALYADIHLFTQLTLI
                     LQPTGKCLQWTEHHSQRVPG"
BASE COUNT         1174 a         1504 c         1455 g         1199 t
ORIGIN      
        1 tctctttttt gtacacaaag actcctttta aaaaatagca aggtcacata tcccttattg
       61 gtacataaat cccccgatgt ttactgactt tgttccacct tcgtatccag taaaatgaca
      121 tagcgattca gcaactgatt taaccgggta tcaatctgcg tcaacagcgt cggcgaaatc
      181 tctccggtct gtttttgctc ttccagttgc cgaagcagtt cttccagcgg cggctccttt
      241 aacgactgtc ggatgccgta caccgccgtt tttagttccg aaccggtcag atatctcccc
      301 ttacgctcat ccagtgcatc cagttgtgcc gacaggcgct gtagctgact ctgcgcctgt
      361 gcgtagccat tcagtgcctc tgcggacagg gcgctcagtt cccgctgttt acgccactgt
      421 gcgttcagcg ccctgacctg aggattatcc ggccacaggg catcaagctg gcgtagctgg
      481 cgatatccct gctccagcgg ccagcgggca ggtaatttgt ttaactgctc cagttgcatc
      541 tgacttgccg caattgttct gtcctgagcc agcagcgcct tatcctttac atccagcttt
      601 gccagttcgc cgctctccag cgactgcggc aacggcatga tagacagttg tattcgctgg
      661 attagcgcgt ccggctgatt aagctgcgac caaacccata atcctgttcc gccgagacag
      721 gccatcacaa ccattccggc cataaatatc ggccatgccc gctgccggga gatttcaggg
      781 gatgacacgg gtggactggc atccttatcg ttttgctgag cgggtctcgt tgcaaaaacc
      841 ggctgtggac tttcttctga ctttttcggt tgattcattt ccctgcgggg taattcaggg
      901 ggagtgatag tggtttccgc tccctgaggc gccagccgtt ccagacgcaa tgcggtattg
      961 cgaaactgcc cggccagcac atccagtttc gtcatatgcc acagttcaca ctgttgcagc
     1021 gtggtttcga tggcggaaag atgctgtaca caacggtaga tctgcggcag atcctgatac
     1081 tgaatatcca gcgttctcag tgcctgctgc attttttctg tgacccagct gagcagtgat
     1141 attctggaat gtacctgtac cggccagaag tcagcccagc gctggagcag tatatccagt
     1201 gagccgagtc cgtctgccat ccctgccagc ccctgccgtc tggttatcgc cagtacgtaa
     1261 cagaccagcg tctgtagttc cacgccattt tgccggaaca gcgcgaggca gagtttttcc
     1321 acctgacgcc agtccacatc cggtcgggcc gggtgagtca gtttactcat ttcagcctgc
     1381 agttcaccgg attccttata agtacaggga tcgccgccca tgacgaactg gctgataaaa
     1441 ttcgcgttac ttgccatcag tacgcccgct cctgttgttt gagatgacgc agaacaattc
     1501 gtccttccgg agaaaatacg ccatcaaagc gtatccagcc ggtatcggga agctgcgcgt
     1561 ggatcaggta attaagatgc cccggacggg gctgggggag taactccacc tgtatctgac
     1621 tcaggcgtgg ttcataacgc agcagcgttt cttcaatctg acgcatcagg ccatgaatac
     1681 ccgcagccat cccctgatgg atcagactga gatccggcag accgtaatcc ggcaaatgcg
     1741 ccagcgtgcc tgcccggcag ttgataatgc gttgcatatt gtccatcacg gaaaggataa
     1801 gctgatcccg ctctctgacc tgttccagcg gtaactcgcc gctgaaactg aacgtcagca
     1861 tttcatataa ggaaggtgtc ggcatcggtt atttatcctt gagaggcagc agtcgcaggt
     1921 cgcttctcat cagttcaatc gagcgcggct tgtcggcctc cagttcgtcc cttttgatca
     1981 ccagacgcca gctgtcgctt ttttcatcag ggtgataaaa ctgcccaata atggcgacaa
     2041 actgcgtctc tttatccagc ggcacattca gggatgccgt gccttccggt tttacccaga
     2101 cggtgtgttt tgccagtacg ttcttctcca gcgtcttctc ttcctgcata aacagggtgt
     2161 cataatccgc cttgtcaaaa tcttcgcggg ttttcagttg ccagacatgc accatcgtgg
     2221 aaagcggcgt atcttccgca tccgtattca gggcacttcg ggagaagaaa tcgagatgca
     2281 gtattttgat ttgcttgtag aaaacggctg aggccacgct tttcgattca tccaccactt
     2341 tctgaatcag cccgcagccg ctcagaacaa ccgtcagcat cgccatacag agcagcgccg
     2401 gacgtttaaa acgaatagga aacatgagtc atactctccc tgttacgaat ggaaaaagcg
     2461 ggtgttaatc cctgataacg ccccagactg accgtcactg ttccggtcgc gggcgcggca
     2521 aaggaggcgc gaaggatgcc ggtacggcta atctgtacac gctgttttcc cagccgggca
     2581 gcaggcaaca ggctgacggg cagcgtcagt tgcagtcggg cctgacagcg ccagcccaga
     2641 taaacgcgca gcagcaccag caaatcgctg tgcagttgcc cgccgggcag ccagccacgt
     2701 gcttctgcgg catcttcggt atacagtttc agcagcaact ggctgttgac gtcggtgccg
     2761 gtacgcccca gcagcgttcg cgttttcagg ctgatgggtg aattttttga cagacccgca
     2821 ggggccggga gaattacagg ctgcggatca tgaggcacca ccgtcgcttt cgtcagcggg
     2881 gcaagcagtc ccaccagtgc cgtaacgcct tccgccgtgc gcgtcggcag gcgcatcacg
     2941 ctgagcaggg ccagaaaacg ggataccggc gtggcgatat ggttctcgct gccgggaata
     3001 cccagcccga tgagtcccag caggcaacgg gaaatgtcat ccactgcacc tgcttcaaac
     3061 gacgccgggt agttatattt gcgccagatg cggtaatact gggtgataaa gcgatggtta
     3121 aaaatatcga gaaacgccat caccgcgtca tgtccgtcat gacgctgggt aatgtagtcc
     3181 agataggcgg ttggcagtgg cgaatccacg ccgtacaggc cgagaaaagt ggtgcgcact
     3241 gtgggtggcg cgtcgggatt gtccacgtca cgttcaacgt tctttaactc gctgaccgga
     3301 aagcccatcc ccggatgagg acgaaagcgc accgcatcgg cggcggggct gtcggtactg
     3361 cccagcggcg cgttctgctg gcgctgttcc agcaactggc aaaaacggta aaaattgatc
     3421 cgcgcaatat ccggggccag ccggtcggtc agcccggtac gcgctggctg tgatgctctg
     3481 tccattgcag gcactttcct gtgggttgca gaattaaggt tagttgagtg aataagtgga
     3541 tatcggcata cagtgcgaag aagcggtgca gcagttcacc aaacagcgtg atatcacctt
     3601 ctccggcaaa gccgttactg tccagcgtca cctgaatatc cacgccacgc agcagaaagc
     3661 ctctttcaaa acgctgtatc aggctgtgct gaacgtcgac aatcgccgcc agacgacggc
     3721 ggttcatctc gctttcggtc cagtcgtaca gcgccagcgt gccgcgcaga atttcggcgt
     3781 tatccatcat cgataaaaag ttagaaccaa ggtggctgag cacccgccag tggaagcggt
     3841 cacgcgccgg gggatagcag ggctgcgtcg gggcgcacag gttgcgcact cgcaggacgt
     3901 tctgtgaggc atgaactggc gtatcgagca gcgtgctctg taaagcttta cggggaagct
     3961 gcccgttagt accggtcagg cttaaggaca gggtttcatc ctccagcatc cggtcagtat
     4021 caaacgcatc gccgcccaga atcagccagg tgtcatgcag gccggaaggg ccgcgcttta
     4081 cccgcgtgtg gtaatagcgt tccggcgcat cgtggcgcag catcccgccc cggtgacgaa
     4141 agctggagaa tggcacatac gcctgactgc cggtatggcg cgaagaaata atgttgtcga
     4201 cggaataaat ctcggtatgc ccgtcctgaa tgcgcatcgg gcgcagcaga aattcgtttt
     4261 ccagtggcga aaggtgtaac ggatcggctt ccagcggaaa caggttgatg accggcacgc
     4321 agtgcagacg gatattatcg ctgtcaaacg gcaggtcgtg aggccagttc tcgttcagca
     4381 acacgtcaat ctcaaagccg gttatcccct ccggccatgc aacctgctcc agcccgttca
     4441 gcgccacgaa cataaacttc tcgcggaagg tgaaatactc cagcagcaac tggtagccgc
     4501 tgaacgccga ttcgcctttc ggccacagtc ggtcatcgtc atcaaaaccc atcggggaaa
     4561 agtgcgcgtc cagcacacgg cgtccctgcc ctggcagacg cagccagaac tgctgcgtac
     4621 caagcgttaa agcccggtgc agcgcacagg ccaccgggct gtcggcactg aggtacagcg
     4681 gcaggcggga taaatcaatc tgtgaccagt caccaaccag cgggccgcat tcaaagcgca
     4741 gacggataac cgagcgtccg tccggttcat attgcaatga cacgtccggc aacgccagcg
     4801 gatgcagcgt gatatcccgc gtggcgctgt agacgcagcg ggttctgcgt tcgccgatgg
     4861 gacgggatag cacctgaaac tctttcagcg tatcactttg cttcatttgt tggtgatccg
     4921 tggtcagttc gaccacggaa agcgacggaa tagtgcgcag gtagtgcggc cacagcaggc
     4981 tgaccagccc ttcagtcagt tccggcagat cgtcgtccag cttttcgcgc aggcggccca
     5041 ttaaaaaagc gaagccctcg aacaggcgct ccacataggg atcgcgtgcg ccgggtttat
     5101 caagattaag catcgcagcg cggtcggggt aagcctgcgc aaattctttc cccgcttcac
     5161 gcaggtagcg catttcggcc tcaaagtagc gttgggtcag gtcgtccatc ggaggattta
     5221 tctttccttt tattgaaatt aataccaacg agtagtgaga ttagaatttt ttattttcag
     5281 aatcctaagg acatatagat ggatcattta tttcaaaaga aaaatcggac tc
//
LOCUS       DAHHTG010000208         5324 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.212, whole genome shotgun sequence.
ACCESSION   DAHHTG010000208 DAHHTG010000000
VERSION     DAHHTG010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5324)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5324)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5324
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.212"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            complement(40..1323)
                     /gene="aroA"
                     /locus_tag="NBI74_004350"
     CDS             complement(40..1323)
                     /gene="aroA"
                     /locus_tag="NBI74_004350"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001764391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HCD1934576.1"
                     /translation="MESLTLQPIARVDGTINLPGSKSVSNRALLLAALAHGKTVLTNL
                     LDSDDVRHMLNALTALGVSYTLSADRTRCEIIGNGGPLHAESARELFLGNAGTAMRPL
                     AAALCLGSNDIVLSGEPRMKERPIGHLVDALRQGGAKITYLEQENYPPLRLQGGFTGG
                     NVDVDGSVSSQFLTALLMTAPLAPEDTVIRIKGDLVSKPYIDITLNLMKTFGVEIENQ
                     HYQQFVVKGGQSYQSPGTYLVEGDASSASYFLAAAAIRGGTVKVTGIGRNSMQGDIRF
                     ADVLEKMGATICWGDDYISCTRGELNAIDMDMNHIPDAAMTIATAALFAKGTTTLRNI
                     YNWRVKETDRLFAMATELRKVGAEVEEGHDFIRITPPEKLKFAEIATYNDHRMAMCFS
                     LVALSDTPVTILDPKCTAKTFPDYFEQLARISQPG"
     gene            complement(1394..2482)
                     /gene="serC"
                     /locus_tag="NBI74_004351"
     CDS             complement(1394..2482)
                     /gene="serC"
                     /locus_tag="NBI74_004351"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HCD1934577.1"
                     /translation="MAQIFNFSSGPAMLPAEVLKQAQQELRDWNGLGTSVMEVSHRGK
                     EFIQVAEEAEKDFRDLLNVPSNYKVLFCHGGGRGQFAAVPLNILGDKTTADYVDAGYW
                     AASAIKEAKKYCTPNVFDAKVTVDGLRAVKPMSEWQLSDNAAYMHYCPNETIDGIAID
                     ETPDFGKDVVVAADFSSTILSRPIDVSRYGVIYAGAQKNIGPAGLTIVIVREDLLGKA
                     NIACPSILDYSILNDNDSMFNTPPTFAWYLSGLVFKWLKANGGVAAMDKINQQKAELL
                     YGVIDNSDFYRNDVAKANRSRMNVPFQLADSALDKLFLEESFAAGLHALKGHRVVGGM
                     RASIYNAMPLEGVKALTDFMVEFERRHG"
     gene            complement(2681..3373)
                     /locus_tag="NBI74_004352"
     CDS             complement(2681..3373)
                     /locus_tag="NBI74_004352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HCD1934578.1"
                     /translation="MKAFDLHRMAFDKVPFDFLGEVALRSLYTFVLVFLFLKMTGRRG
                     VRQMSLFEVLIILTLGSAAGDVAFYDDVPMVPVLIVFITLALLYRLVMWLMAHSEKLE
                     DLLEGKPVVIIEDGELAWSKLNNSNMTEFEFFMELRLRGVEQLGQVRLAILETNGQIS
                     VYFFEDDKVKPGLLILPSDCTQRYKVVPESADYACIRCSEIIHMNAGEKQLCPRCANP
                     EWTKASRAKRVT"
     gene            3503..5263
                     /locus_tag="NBI74_004353"
     CDS             3503..5263
                     /locus_tag="NBI74_004353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     accessory protein YcaO"
                     /protein_id="HCD1934579.1"
                     /translation="MTQTFIPGKDAALEDSIARFQQKLSDLGFQIEEASWLNPVPNVW
                     SVHIRDKECALCFTNGKGATKKAALASALGEYFERLSTNYFFADFWLGETIANGPFVH
                     YPNEKWFPLTENDDVPEGLLDDRLRAFYDPENELTGSMLIDLQSGNEDRGICGLPFTR
                     QSDNQTIYIPMNIIGNLYVSNGMSAGNTRNEARVQGLSEVFERYVKNRIIAESISLPE
                     IPADVLARYPAVVEAIETLEAEGFPIFAYDGSLGGQYPVICVVLFNPANGTCFASFGA
                     HPDFGVALERTVTELLQGRGLKDLDVFTPPTFDDEEVAEHTNLETHFIDSSGLISWDL
                     FKQDADYPFVDWSFSGTTEEEFATLMAIFKKEDKEVYIADYEHLGVYSCRIIVPGMSD
                     IYPAEDLWLANNSMGSHLRETILSLPGSEWEKEDYLNLIEQLDEEGFDDFTRVRELLG
                     LATGSDNGWYTLRIGELKAMLALAGGDLEQALVWTEWTMEFNSSVFSPERANYYRCLQ
                     TLLLLAQEEDRQPLQYLNAFVRMYGADAVEAASAAMSGEAAFYGLQPVDSDLHAFAAH
                     QSLLKAYEKLQRAKAAFWAK"
BASE COUNT         1368 a         1362 c         1255 g         1339 t
ORIGIN      
        1 gaaaagaaag gattgtctat gttatcgccc gttattcatt cagccgggtt gacttatccg
       61 cgccaattgc tcgaaatagt ctggaaaagt tttagcggta cattttggat caagaatcgt
      121 cacaggcgta tctgataatg cgaccagcga gaaacacatc gccatccggt gatcattata
      181 agtggcgatc tcagcaaatt tcagtttttc cggtggcgta atgcgaatga aatcatgccc
      241 ctcttcgact tccgcaccga ctttacgcag ttctgttgcc atcgcaaaca ggcgatcggt
      301 ctctttaaca cgccagttat agatattgcg cagcgtggtg gtgccttttg caaataacgc
      361 cgcggtggca atggtcatcg ccgcatcggg aatatggttc atatccatat caatggcgtt
      421 cagttcacca cgcgtgcagg aaatataatc atcgccccag caaatggtcg cgcccatttt
      481 ttccagaaca tcagcaaagc gaatatcacc ctgcatactg ttgcgtccaa tgccggtcac
      541 ttttacagtg ccgcctctga ttgctgctgc tgccaggaaa taagaagccg aagatgcatc
      601 gccttcaacc aaataagtgc ccggagactg gtaagactgc ccccctttta cgacaaattg
      661 ttgatagtgc tgattttcaa tttcaacacc aaacgtcttc atcagattga gggtgatatc
      721 gatataaggt ttagaaacca gatcgccttt aatacgaatc accgtatctt ccggcgcaag
      781 aggcgcagtc attaacagtg cggtgaggaa ctggctggaa acggagccat caacatcaac
      841 gttgccgcca gtaaagccgc cctgtaaacg caatggcgga taattttctt gttccaggta
      901 agtgatcttc gccccgccct ggcgcagcgc atccaccaga tgaccaatcg ggcgttcttt
      961 cattcgcggt tcaccgctca gtacaatatc attgctaccc aggcaaagtg ctgccgccag
     1021 cggacgcatt gccgttccgg cgttaccgag gaacaactcc cgggcacttt ctgcgtgtaa
     1081 tggaccaccg ttaccgataa tttcgcaacg cgtacgatcg gctgaaagcg tatagcttac
     1141 ccctaacgct gttaatgcat tcagcatatg gcgcacgtca tcgctatcca gcagattggt
     1201 taatactgtt ttgccgtgtg ctaatgccgc cagcaataaa gcgcggttag aaacgctctt
     1261 ggaaccgggc agattaatag tgccatcgac acgagcgatg ggttgtaacg ttagggattc
     1321 catgaactca actctctaca acagaaataa aaaccccaca ggctggctgt ggggattatg
     1381 caaaatttcg acattagccg tgacgacgtt cgaactcaac catgaagtct gtcagtgctt
     1441 taacaccttc cagcggcatg gcgttataaa tagacgcacg cattccgccg accactcggt
     1501 gacctttcag cgcatgtaag cccgcagcga aagactcttc gaggaacaat ttatcaagcg
     1561 cgctatccgc caactggaac ggcacgttca tccgcgaacg gttagctttc gccacatcat
     1621 tgcggtagaa atcgctgtta tcaatcaccc catacagcag ttctgctttt tgctgattga
     1681 tcttatccat tgcagccaca ccgccgttcg ctttcagcca tttgaagacc aggccagata
     1741 agtaccaggc aaatgtcggc ggcgtgttga acatggaatc gttatcgttg aggatggagt
     1801 aatccagaat cgatgggcag gcgatattcg ctttgcccag caaatcttca cgaacgatga
     1861 cgattgtcag gccagccggg ccgatatttt tctgcgcgcc agcgtaaatt acaccataac
     1921 ggctgacatc aatcggacgg gaaagaatgg ttgaagagaa gtcagcggcg accaccacgt
     1981 ctttaccgaa gtctggtgtt tcatcgatag cgataccatc gatggtttcg ttcgggcagt
     2041 aatgcatata agcagcatta tcagagagtt gccattcact cattggctta actgcgcgaa
     2101 gaccatcaac ggtcactttc gcatcaaaca catttggtgt gcagtatttt ttcgcttctt
     2161 tgatagcact tgctgcccag taacccgcat caacataatc tgcagtggtt ttatcaccaa
     2221 gaatattcaa cggtactgca gcaaattgac cgcgaccacc gccatggcag aacaatactt
     2281 tgtagttgga ggggacgtta agaagatcgc gaaaatcctt ctcggcttcc tctgcaacct
     2341 gaatgaactc tttgccacgg tgactcactt ccatcaccga cgtaccaaga ccgttccagt
     2401 cgcgcagttc ctgttgagcc tgtttaagca cctctgccgg tagcattgcc ggaccagaac
     2461 taaaattgaa gatttgagcc atttcccctc accacgttgc gttgctatta acccgcggtc
     2521 aatcgattgc gaccacaggt aatacattat tccagtggct atcggtttta tcattcagtg
     2581 acacacatcg caatgagtaa aaccgttgag ggcaaaaatg tggcttgcat cacacaaaag
     2641 aatctctcgg attattagca aaaagccgat gtttttgctg tcaggtcacc cgtttagctc
     2701 gacttgcctt cgtccattct ggatttgcac agcgcggaca taattgtttt tcccccgcgt
     2761 tcatatgaat gatttcactg caacggatgc aggcatagtc cgccgactcc ggcaccactt
     2821 tgtaacgctg agtacagtca ctgggtaaaa taagtaaccc cggtttcacc ttgtcatctt
     2881 caaagaaata aacactgatt tgcccgttgg tttcgagaat cgccagacgt acctgcccca
     2941 actgctctac accgcgcaat cgtagctcca taaagaactc aaattccgtc atgttggagt
     3001 tattgagttt cgaccaggcc agctcgccat cttcaataat gacaaccggc ttgccttcca
     3061 gaagatcttc cagtttttca ctgtgcgcca tcaaccacat taccaggcgg tataacaacg
     3121 ccagagtaat aaagacgata agtaccggga ccatcggcac atcatcataa aacgccacat
     3181 ctcccgccgc tgaacccagt gtcagaatga ttaacacttc aaacagcgac atctgccgca
     3241 caccgcgtct tccggtcatt ttgaggaaca aaaaaaccag tacaaaggta taaagactac
     3301 gtagtgcaac ttcgccaagg aaatcaaaag gcactttatc aaatgccata cggtggagat
     3361 cgaacgcctt catttttatg ctcttatcgt ggtttatccg ctaaaagcat agccaacagt
     3421 tcatcggcat ggcggcggtg atagcgcctt tgagataaaa cccgtattat tgcgcgcttt
     3481 ccgtacgact aaagtgattt tcatgacgca aacatttatc cccggcaaag atgccgctct
     3541 ggaagattcc atcgctcgct tccagcaaaa actttcagac ctcggttttc agattgaaga
     3601 ggcctcctgg ctgaatcccg tgcctaatgt ctggtctgta catattcgcg acaaagagtg
     3661 cgcactgtgt tttaccaacg gtaaaggcgc aaccaaaaaa gcggcgctgg cttctgccct
     3721 cggtgaatat ttcgagcgtc tctcaaccaa ctactttttt gcggacttct ggctgggcga
     3781 aaccatcgcc aacggcccat tcgtgcatta tcccaacgaa aaatggttcc cactgaccga
     3841 aaatgacgat gtaccagaag ggctgctcga tgaccgtctg cgcgcatttt acgatccgga
     3901 gaatgaactg accggtagta tgctgattga cctgcaatcc ggtaacgaag atcgtggtat
     3961 ttgcggtcta ccgtttacgc gccagtccga caatcagacc atttatattc cgatgaatat
     4021 cattggtaac ttgtacgttt ctaacggtat gtctgctggc aatacccgta acgaagcacg
     4081 cgttcagggg ttatccgaag ttttcgaacg ttacgtgaaa aaccgcatta ttgctgaaag
     4141 catcagcttg ccggaaatcc cggcagacgt gctggcacgt tatccagcag tagttgaagc
     4201 gatcgaaacg ctggaagcgg aaggtttccc gatcttcgct tatgacggtt cgcttggcgg
     4261 ccagtatccg gtgatttgcg tggtactgtt caatcctgct aacggtacct gctttgcctc
     4321 attcggtgcg catcctgatt ttggcgttgc actggaacgt accgtgaccg agttgctgca
     4381 aggtcgtggc ctgaaagatc tggatgtgtt tactccacca accttcgatg atgaagaagt
     4441 cgctgaacat accaacctcg aaacgcactt tatcgattcc agcggtttaa tctcctggga
     4501 cctgttcaag caggatgccg attatccgtt tgttgactgg agcttctccg gcaccacgga
     4561 agaagagttc gccacgctga tggctatctt caaaaaggaa gataaagaag tttatattgc
     4621 cgattacgag catctgggtg tctactcttg ccgtattatc gtacctggca tgtccgatat
     4681 ttatccggct gaagatctgt ggttggcgaa taacagtatg ggcagccatt tacgtgaaac
     4741 gattctgtca ctgcccggta gcgagtggga aaaggaagat tacctgaacc tcatcgagca
     4801 actggatgaa gaaggttttg atgactttac ccgcgtgcgt gaactgttgg gcctggcgac
     4861 cgggtcggat aacggctggt acactctgcg tatcggtgaa ttaaaagcca tgctggcgct
     4921 ggctggtggc gatctggaac aggctctggt ctggaccgaa tggacgatgg agtttaactc
     4981 atcagtattc agcccggaac gcgccaatta ttatcgctgc ctgcaaacat tgttattact
     5041 ggcgcaggaa gaagatcgcc agccgctgca atatctgaat gcgtttgttc gcatgtacgg
     5101 cgcagatgcc gtggaagccg ccagtgcagc aatgagcggc gaagcggcgt tttatggctt
     5161 gcaaccagta gatagcgatc tgcacgcgtt tgctgcacat cagtcgttgt tgaaggccta
     5221 cgaaaagctg caacgcgcca aagcagcatt ctgggcaaaa taaaatcaaa tagcctacgc
     5281 aatgtaggct taatgattcg tctgagttat attacggggc gttt
//
LOCUS       DAHHTG010000209         5307 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.213, whole genome shotgun sequence.
ACCESSION   DAHHTG010000209 DAHHTG010000000
VERSION     DAHHTG010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5307)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5307)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5307
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.213"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            281..1132
                     /locus_tag="NBI74_004354"
     CDS             281..1132
                     /locus_tag="NBI74_004354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001195265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HCD1934580.1"
                     /translation="MQKVKLNNGIEMPLLGFGVFQMTDAAECERAVIDAINSGYRLID
                     TAASYQNEIQVGNALKQSGIARNELFVTTKLWLQDTSYEGAKAQFERSLNRLQLDYVD
                     LYLIHQPYGDVHGAWRAMEELQQAGKIRAIGVSNFHPDRLADLIAFNNVVPAVNQVEV
                     NPFNQQLQAVPWMQSRGIQPEAWAPFAEGKNGLFQHPVLTAIGEKYGKSVGQVVLRWI
                     YQRGIVSLAKSVRKERMEENINILDFELSPEDMLQITALDTATSAFFSHRDPAMVEWL
                     TGRKLDV"
     gene            1159..2148
                     /locus_tag="NBI74_004355"
     CDS             1159..2148
                     /locus_tag="NBI74_004355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001172283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HCD1934581.1"
                     /translation="MQKRYLGKSGLEVSALGLGCMGLSHGYGPATDTRQAIELIRAAV
                     ERGVTFFDTAEVYGPYLNEEVVGEALKPFRDRVVIATKFGFTFGDDNKQQILNSRPEH
                     IREAVEGSLRRLKTDVIDLLYQHRVDPDVPIEDVAGTVKDLIAEGKVKHFGLSEAGAQ
                     TIRRAHAVQPVTALQSEYSMWWREPEQEILPLLEELGIGFVPFSPLGKGFLTGAIKPG
                     TTFGKDDYRSTVPRFAAQAIEANEKLVTLLGELAADKGVTSAQIALAWLLAQKPWIVP
                     IPGTTKLNRLEENLAAADIVLSQKDTQQITEALETIKIVGERYSPEHQARVGR"
     gene            complement(2179..3072)
                     /locus_tag="NBI74_004356"
     CDS             complement(2179..3072)
                     /locus_tag="NBI74_004356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016234625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCD1934582.1"
                     /translation="MLKENFNELQIFLVVARERSFTKAAGKLGVSQSALSHAMKALEE
                     RLNIRLLTRTTRSVAPTEAGERIIACLEPRIDELEQELESLIQLNGTPSGNIRLSAGE
                     HAARSLVWPKLKPFLREYPEINVELVVDNGFVDIVEGRFDAGIRLGENVDKDMVAVRI
                     GPDMRMAVVGAPAYFAANPAPETPHELQNHRCINMRLPTAGGLYHWEFEREGKPLRVK
                     VDGQLTCSPLPERIDAALSGFGIACVPEDMVQEYIKSGKLIQVLQEWCPTFPGYYLYY
                     PSRKQHPPAFALLIDALRYTE"
     gene            complement(3272..4198)
                     /locus_tag="NBI74_004357"
     CDS             complement(3272..4198)
                     /locus_tag="NBI74_004357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCD1934583.1"
                     /translation="MMKIEPSILPSLAWFALIVRAGSFSRAASEMGITRAALSQNLKS
                     LEERLNTKLIYRTTRNMSLTEEGQHLYEVLVSALGQIDDALKDVGDTQLEPTGLLRIN
                     SSRVAARMLVEPHIGEFLTRYPKTKIELIMDDGLSNIIADGCDVGIRLEQGLDEHMTA
                     IPVSPLIKLVTVASPDYLKEHGIPETPQELSNHNCLRLRHKSSGALSAWEFSNVVGGN
                     EEFEIEVSGKYISNDDESMIRMALNGTGIIQHLDFAIAEHINAGKLQPILEDWAVSFP
                     GFYIYVSSRVRMPSKVRAFIDFMVEKRVKIES"
     gene            4355..5275
                     /locus_tag="NBI74_004358"
     CDS             4355..5275
                     /locus_tag="NBI74_004358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCD1934584.1"
                     /translation="MKTVSIKHTYWDIAADIYFPDDFNSEKKYPAIISAHPIGSCKEQ
                     TSGSVYGAALAKAGFIVIAFNASFQGSSGGEPRYLEDPTMRVKDFSIVVDYLTTLPYV
                     DAGRIGVLGICGGGGYAINAAMTERRIKAIGTVTGANYGRLMREGFTAFNPIGALEAM
                     AQQRTDEANGAKLRVDDLLPSSPEAALEAGLTEIDLFEATEYYRSPRGCAPNGVNRSL
                     FSHQTVAVGWDAFHLAEVLLTQPLMVVVGDRVGAFGAYRDGCEIIGRAASKHKELVVV
                     EGYSHYDLYDKPEPVKQALDKLIPFYKTHL"
BASE COUNT         1462 a         1278 c         1201 g         1366 t
ORIGIN      
        1 tcattatcca gagtgaataa taatgacccg tctttaatta actgctattg ggagtacgtc
       61 gaagtaagta tatacgccgg gataaaccaa tatatttttt atggacattc atttttattc
      121 atcggactgt aaattccggg gatatcgaac ttgcacgact aatctcaacc ctcaaacaga
      181 tttatgaatt ttgttcataa cccctgacaa atcctgccgt ctaatcgagc tcttcccttt
      241 acgttatgct tctcatgaac gataacggaa ggataacacc atgcaaaaag taaaactgaa
      301 caacggtatt gaaatgcccc tgttgggctt tggtgtattt cagatgaccg atgccgccga
      361 atgcgaaaga gcggtcattg acgctattaa cagcggttat cgactgattg acaccgccgc
      421 ctcttatcaa aatgaaatcc aggtcgggaa cgcactgaaa caaagcggta tcgcccgtaa
      481 cgaactcttt gtcaccacga aactctggtt gcaggatacc agttacgaag gggccaaagc
      541 ccagttcgaa cgctctctga atcgactgca acttgattac gttgacctgt acttgattca
      601 ccagccatat ggcgatgtcc acggagcctg gcgtgccatg gaagagctgc aacaggccgg
      661 taaaattcgc gctattggcg tcagtaactt ccatcctgac cgactggcag accttatcgc
      721 ttttaataac gtcgttccgg cggtaaacca ggttgaagtt aaccctttca atcagcagct
      781 acaggccgtt ccctggatgc aaagtcgtgg cattcagcca gaagcctggg caccgtttgc
      841 ggaagggaaa aatggtctgt tccagcatcc tgttctcacg gcaattggcg agaagtatgg
      901 caaaagcgtg ggccaggttg ttctgcgctg gatctaccaa cgaggcattg tttcactggc
      961 aaaatcagtg cgaaaagaac gcatggaaga gaacatcaac attctcgatt ttgaactcag
     1021 ccctgaagat atgctgcaaa ttaccgccct ggataccgca accagcgcgt tcttctctca
     1081 ccgcgatccc gcaatggtgg aatggctgac tggccgcaaa cttgatgttt aagccgcgat
     1141 aaaagaggta ttcattcaat gcaaaaacgt tatctgggta aatccggact cgaagtctcc
     1201 gcccttgggc tcggttgcat gggcttaagc cacggctacg gcccggcgac cgatacccgt
     1261 caggctatcg aacttattcg tgctgcggtt gaacgtggtg tcaccttctt cgataccgcc
     1321 gaagtgtatg gtccgtatct gaatgaagaa gtggtcggcg aagccttaaa accgtttcgt
     1381 gaccgcgtgg taattgccac taaattcggt tttaccttcg gcgatgataa taagcagcag
     1441 attttaaaca gccgtccgga acatatccgt gaagccgttg aaggatcatt acgtcgtctg
     1501 aaaacagatg ttattgacct gctgtatcaa caccgtgttg atccagatgt tcctattgaa
     1561 gatgttgcag gaacggtgaa agacctcatc gcagaaggta aggtcaaaca tttcggcctg
     1621 tctgaagcag gagcacaaac cattcgtcgt gcacatgcgg tacaacctgt caccgccctg
     1681 caaagcgaat attccatgtg gtggcgtgag cctgaacagg aaattctgcc gttgctggag
     1741 gaactgggaa ttggttttgt ccccttcagc ccactaggta aaggtttcct gacaggggcg
     1801 attaagccgg gaaccacttt cggcaaagat gattaccgca gcaccgtacc gcgtttcgca
     1861 gcacaagcca ttgaagccaa tgaaaaactg gtcaccttgt taggtgaact ggctgcagat
     1921 aaaggggtaa cgtctgcaca aatcgcactg gcatggctat tagcacaaaa gccgtggatt
     1981 gttcccatcc ctggcaccac taaactgaac cggctggaag aaaacctggc cgctgctgac
     2041 attgttcttt cgcagaaaga tacgcagcag ataaccgagg cgctggaaac aattaaaatc
     2101 gtcggtgaac gttactcgcc tgaacatcag gcacgcgtag gccgctaata cccaaacggg
     2161 tccaggataa aggacccgtt attcggtgta gcgaagcgca tcgatcaaca gcgcaaaagc
     2221 tggcggatgc tgcttgcggc tggggtagta aagataatac ccaggaaaag ttggacacca
     2281 ctcctgtaac acctgaataa gcttgcctga ctttatatac tcctgaacca tgtcttcagg
     2341 gacacaagcg atcccaaacc ctgataacgc ggcatcaatt ctttctggca atggactaca
     2401 cgtcagttgc ccatcaacct taacgcgaag cggtttccct tctcgctcga actcccagtg
     2461 gtaaagccca ccggcagttg gcaaacgcat gttgatgcag cgatgatttt gtagctcatg
     2521 aggcgtttca ggcgcagggt ttgcagcaaa atacgccgga gctcccacca cagccatccg
     2581 catatctggt ccaatcctga ctgcaaccat atctttatca acattttcac ccagtcgtat
     2641 cccggcatca aaacggcctt caacaatatc gacaaaaccg ttatccacca ccagttcgac
     2701 attgatttcg ggatactctc tgagaaaagg ttttagcttc ggccagacca gacttcgcgc
     2761 ggcatgttcc ccggcagata accgaatatt cccggagggc gtgccgttca attgaataag
     2821 tgattccagt tcctgttcaa gctcgtcaat acgcggttca aggcaggcaa tgattctctc
     2881 ccccgcttct gtgggagcca cactgcgggt agtccgtgtc aggaggcgga tattgagcct
     2941 ttcctccaga gccttcattg catggctaag tgcagactga gagacgccga gtttgcccgc
     3001 tgctttggta aaacttcgct ctcttgccac cacaagaaag atttgcagtt cgttgaagtt
     3061 ttctttgagc attggcgatt tccttgtaga agcatcctct tcacagacac tcatgaaggg
     3121 gggcatagat gctattattt taaccacatt attagtaaca atgataatct ggctgtatcg
     3181 aaaataattc taaactttca ggagatttat tatgaataaa cgtattctca cgattgcgcc
     3241 attcttctta ccgatattct gacacacatc attaactttc tatttttact cttttctcca
     3301 ccataaaatc aataaaagca cgaactttag agggcattct cacccgagat gaaacatata
     3361 tataaaatcc aggaaatgaa acggcccaat cctcaagaat cggctgtaac ttgcccgcat
     3421 tgatatgttc agcgattgca aaatccagat gttgaatgat acccgttcca ttcagcgcca
     3481 ttcgtatcat gctttcgtca tcattagaaa tatacttacc tgacacctct atttcgaact
     3541 cttcattacc ccccacaaca ttcgaaaact cccatgctga gagtgcaccg ctacttttat
     3601 gccgcaaccg aagacaatta tggttactca attcctgagg agtttcagga ataccgtgtt
     3661 cttttaaata atctggcgat gccacagtaa ccagtttaat aagcggagaa acaggaatgg
     3721 cagtcatatg ctcatccagt ccttgctcaa gacgtatgcc cacatcacag ccgtccgcaa
     3781 taatgttaga taaaccgtca tccataatga gttctatctt tgttttgggg taacgagtaa
     3841 gaaactcgcc aatatgaggc tcaacaagca tccttgctgc aacacgggag gaatttattc
     3901 tcaacagtcc tgtaggttca agttgggtat caccgacatc tttcaacgca tcatcaattt
     3961 gccccaaagc cgacaccagc acttcataca gatgctgtcc ttcctcagta agcgacatat
     4021 tgcgggttgt acggtatatc agcttggtgt taagacgttc ctcaagagat tttaaattct
     4081 gggacaatgc ggcccgcgta atacccattt cagaggctgc tctggaaaaa ctgccggcac
     4141 gaacaatcag cgcaaaccag gcaagggaag gcaaaattga aggctctatt ttcatcattg
     4201 taaaccttaa cttaacactg tgatagatat tagaccattg atgataacca atacaagtca
     4261 tactattacg ccgttcgcga tatgaacacg aatgccagtt gcggataata gaaatgatat
     4321 ttaaatctgt ttaggcaaat ccctggagct tgaaatgaaa actgtttcaa taaaacacac
     4381 ttactgggat atagctgctg atatctattt ccctgacgat tttaatagtg aaaaaaagta
     4441 tcctgccatt atcagtgccc atccaattgg aagctgcaaa gaacagacat cgggtagtgt
     4501 gtatggtgcc gccctggcaa aagctggctt tatcgtcatt gcttttaatg ccagttttca
     4561 gggaagcagc ggtggagaac cgcgttatct ggaagatcca acaatgcgag taaaagactt
     4621 cagcattgtc gttgattacc ttaccacctt accttacgtt gatgccgggc gtattggtgt
     4681 actgggtatt tgtggtggag gtggatatgc cattaatgcc gcaatgacag aacgtcggat
     4741 taaagccatt ggtactgtca caggtgcaaa ctacgggcgt cttatgcgtg agggatttac
     4801 agcgtttaat cctatcggag cactcgaagc aatggcgcaa caaagaacag acgaagctaa
     4861 cggcgcaaaa cttcgcgtag atgatttgct tccttcttct ccggaagcag ctcttgaagc
     4921 gggattaaca gaaatagacc tttttgaagc aacagaatat tatcgttcgc cacgaggctg
     4981 tgcgcctaat ggggtaaatc gttcgctatt ttcacatcag actgttgccg tgggctggga
     5041 tgcttttcat cttgcagaag tcctgttaac tcaaccacta atggttgtag taggcgatag
     5101 agtgggcgct tttggtgcct atcgtgacgg ctgcgaaatt attggtcgtg cagcctcaaa
     5161 acataaagag ttggtcgtgg ttgaaggtta ttcacattac gacctgtatg ataagccaga
     5221 acctgtgaaa caggcgctcg ataaattaat tcctttttac aaaacacatt tgtaataacc
     5281 ttgcagggag attcattatc tccctga
//
LOCUS       DAHHTG010000210         5286 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.214, whole genome shotgun sequence.
ACCESSION   DAHHTG010000210 DAHHTG010000000
VERSION     DAHHTG010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5286)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5286)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5286
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.214"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            155..1000
                     /locus_tag="NBI74_004359"
     CDS             155..1000
                     /locus_tag="NBI74_004359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020245455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HCD1934585.1"
                     /translation="MNNITVKSLIQSNYPTLHQAEKKVADYILSHAHEVVNYSVTELS
                     EKSHASEATIVRTCKKLGYQGYYHLKIALAKEVINPDNSYPENTDFSDITSLATFLLK
                     KQAEDLIQSTQFFNADVLESILKLLANCDTIFFFAAGNSNPLAVYSAYKFSQLGLKTV
                     VHVSPEMQINAAYSMGKRDLAIGISNSGSTNLMVDIFKVVKERGAKSVCITNYIKSPL
                     SKLSTHQLNTAVSDKIFFEAFDSTRVPAMGVIDMLVLLFMYKNKAHYEKYRTREEFLG
                     SRFKG"
     gene            complement(1030..3027)
                     /gene="tkt"
                     /locus_tag="NBI74_004360"
     CDS             complement(1030..3027)
                     /gene="tkt"
                     /locus_tag="NBI74_004360"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001088080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HCD1934586.1"
                     /translation="MNSQLLANAIRMLSVDAIQKANSGHPGAPMGMADIAEVVWRRHL
                     RHNPKNPQWFNRDRYVQSNGHGSMLIYALLHLTGYDLSMDDIRDFRQLHSRTPGHPEY
                     GYTPGVETTTGPLGQGVANAVGMAIAEKALAAEFNKPGFNIVDHHTWLFLGDGCLMEG
                     ISHEACGLAGTLKLGNLIAIWDDNGISIDGHVEGWFAEDTAARFRAYGWHVIEGVDGH
                     DPEAVDAAVREAKSVTDKPSLLCCKTIIGFGSPNKANSHDCHGSALGADEVALVRERL
                     QWPYAPFEIPGEIYAEWDATEKGAQVQQEWDALFADYAKQWPELAAEFTRRMKGDLPA
                     GWVENMQKYVHDLQSHPAALATRQVSQKCLNHFADMLPELMGGSADLSPSNLTRHQKS
                     VDFTGENPAGNYISYGVREFGMSAIMNGLALHGGFIPYGGTFLMFMEYARNALRMAAL
                     MKIRSVFVYTHDTIGLGEDGPTHQPVEQLASLRLTPNMETWRGCDQVEVAVAWQQAIE
                     RKDGPTSLVLTRQPLAQQPRTAAQLAEIARGGYVLSDCDGQPEMILISAGSEIELVVS
                     AAKALTEEGRKVRVVSMPCTERFDNQDAAYKESVLPKAVRKRLAVEASIAGFWERYVG
                     LDGKVIGMTSFGESAPANVLFKHFGFTPENVLAQARELLNS"
     gene            complement(3078..4586)
                     /locus_tag="NBI74_004361"
     CDS             complement(3078..4586)
                     /locus_tag="NBI74_004361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001546794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HCD1934587.1"
                     /translation="MSKKITSLFNFKKLQKFSSALMLPIAILPAAGLILAIGISFNIE
                     LLANCGGLIFANLPLLFCVGIVIGLTGDGSSSLSAIISYMIMNATMGTQLGITAESVA
                     ASNGDYAMILGVPTLQTGVFGGLIVAIFVYLLYTRFHNIELPQFLGFFGGKRFVPIIT
                     AVAAIIVGYLLPWLWIPVQKGLASLSTLVTSGHNANFAAFIYAVGERSLIPFGLHHIW
                     NVPFYYNFGDYMTKSGQLVTGDIPVFFAQLRDGAPLTAGLFMTGRFPIMMFALPAAAL
                     AMYQEAKPERKALIKGLLLSGALTTFVTGITEPLEYAFLFAAPVLYIIHIFLYATSFV
                     LMNILNVHIGHPFAGGLIDFVLNGVMPNRTPWYLVFLAGAGYAAIYYTVFRVLIRKLN
                     LKTPGREDEEIAVATTLTREERPYQIIEAVGGFDNIEDVDACATRLRLALVDDKKVNE
                     KRLKELGAAGLVKLGDGGVQVIFGGKSQILRDEIKTVMSRPRPTEMTVACAN"
     gene            complement(4628..5185)
                     /locus_tag="NBI74_004362"
     CDS             complement(4628..5185)
                     /locus_tag="NBI74_004362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000339043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HCD1934588.1"
                     /translation="MDAKLYITEIINELTFVKDSIREDDCTKLIDTIQQSQRVFCYGL
                     GRAGFSMKAFTMRLMHMGKEVYFLTETITPNFGPGDLFIVSSASGETAQLVALAKKAR
                     QLGGAVAVLTTNRHATITEFVDVIVQINAPSKNQKDSVFRSAQPMASLYEQALLVIAD
                     ALVMKMAAESGAPESELFKRHANLE"
BASE COUNT         1412 a         1429 c         1177 g         1268 t
ORIGIN      
        1 gtttaaatta tttttgtgca gttgacgtaa ttcatgaaaa ttaaccggtc tgttaatcat
       61 tacgttcact gcacaccttc attttttcac acctatccac gcctgaaaac tatgttataa
      121 tcagcacttt ctgtcagaat gaaatttact aattatgaat aacataaccg taaaaagtct
      181 gattcaaagc aattacccta cactgcatca ggcggaaaaa aaggtcgctg actatattct
      241 tagccacgca catgaagtcg tgaactatag tgtcactgaa ctatcagaaa aaagccatgc
      301 cagtgaagcg actatcgtac gcacctgtaa gaaattaggt tatcagggtt attaccattt
      361 aaaaattgca ttagcaaaag aagtcattaa tcctgacaat agctatcctg aaaataccga
      421 cttcagcgat ataacatctt tggcaacctt tttattaaaa aaacaggccg aagatttaat
      481 acaatcaacg caatttttta atgccgacgt gcttgagtcc attctcaaac tacttgctaa
      541 ttgcgatact attttctttt ttgctgccgg aaattcaaac ccactggccg tttatagtgc
      601 ttataaattc agccaactcg gattaaaaac cgttgttcac gtcagcccgg aaatgcaaat
      661 taatgcggca tattctatgg ggaaacgcga cctggcgatt ggtatttcta actctggcag
      721 caccaactta atggtcgata tctttaaagt ggtgaaagag cgcggtgcca aatccgtttg
      781 tatcactaac tatattaaat ctccgttatc aaaactctcc acccatcaac tcaataccgc
      841 ggtcagcgat aaaatcttct ttgaagcctt cgactccact cgcgttccgg caatgggcgt
      901 tattgatatg ctggtgctgt tgtttatgta taaaaacaag gcgcattacg agaaataccg
      961 cacccgtgaa gagtttcttg gctcccgttt caaggggtaa aaggtcatcc tttcacccct
     1021 tgtgcaggtt tagctgttca gtagctcacg cgcctgcgcc aggacatttt ccggcgtaaa
     1081 accaaagtgt ttgaacaaca cattcgccgg agcagactca ccaaagctcg tcatgccgat
     1141 cactttaccg tccaggccca cgtagcgttc ccagaatccg gcgatgctgg cttctaccgc
     1201 cagacgtttg cgtacagctt ttggcaatac cgactctttg tacgccgcat cctgattatc
     1261 gaaacgctcg gtacacggca tggaaaccac gcgaacttta cgcccttctt ccgttaacgc
     1321 ttttgcggcg ctgaccacta actcaatctc tgaacccgca gaaatgagga tcatctcagg
     1381 ctgaccgtca caatcactca gtacataacc accacgcgca atctctgcca actgtgcagc
     1441 ggtgcgcggc tgttgcgcca gaggttggcg cgttaatacc aacgacgttg ggccatcttt
     1501 gcgctcaatc gcctgttgcc aggccaccgc cacttccacc tgatcgcaac cgcgccaggt
     1561 ttccatattc ggcgtcagac gcagcgacgc cagttgctca acgggctgat gtgtcgggcc
     1621 gtcttcgccc aggccgatgg tatcgtgggt ataaacaaac accgagcgga tcttcatcag
     1681 cgccgccatg cgcagtgcat tacgcgcata ttccataaac atcaggaaag taccgccgta
     1741 agggataaac ccaccatgca gcgcaagacc gttcatgatg gccgacatac caaattcacg
     1801 cacgccatag gagatgtaat tccccgccgg attttctccg gtgaaatcaa ccgatttctg
     1861 atggcgagtc aggttagagg gcgacaagtc cgccgagccg cccatcagtt ccggcagcat
     1921 atctgcgaag tgattgaggc atttctggct aacctgacgg gttgccagcg ccgccggatg
     1981 ggattgcaga tcgtgcacat atttctgcat attctcgacc cagccagcag gcaaatcacc
     2041 cttcatgcga cgagtaaact ccgccgccag ttccggccac tgcttcgcgt agtcggcaaa
     2101 cagtgcatcc cattcctgtt gaacctgcgc gcctttctcg gtcgcatccc attcggcata
     2161 aatttcacca ggaatttcga aaggggcata cggccattgc agacgttcac gcaccagcgc
     2221 cacttcatct gcacccagcg ccgagccgtg gcaatcgtgg ctgttggctt tatttggcga
     2281 accaaaaccg ataatcgttt tgcagcacag cagactgggc ttatccgtga cgcttttcgc
     2341 ttcacgcacc gcagcatcta ccgcttctgg atcgtgacca tcaacgcctt caatgacatg
     2401 ccagccgtag gcacgaaaac gggcagccgt atcttcagcg aaccagcctt ctacgtgacc
     2461 atcgatcgaa ataccgttat cgtcccagat agcaatcaga ttgccgagct tcagcgttcc
     2521 tgcaagacca caggcctcgt gggagatccc ttccatcaga cagccatcac ccaaaaataa
     2581 ccatgtgtga tgatcaacaa tattaaagcc cggtttgtta aattctgccg ccagcgcctt
     2641 ctccgcaatc gccatcccca ccgcattagc tacgccctgc cccaacgggc cggtggtggt
     2701 ttcaacaccc ggcgtataac catattctgg atgacccggc gtgcgggagt ggagctgacg
     2761 aaagtcgcga atatcatcca tcgacaggtc gtagcctgta agatgcagca aagcgtaaat
     2821 caacatggag ccgtgaccgt tcgactgaac atagcggtcg cggttaaacc actgcggatt
     2881 ttttgggtta tgacgcaggt gacgacgcca gaccacttcg gcaatatccg ccatccccat
     2941 cggcgcgccc ggatgaccgg agttcgcttt ctggatcgca tcaacactta acattcggat
     3001 ggcattagcc agtaattgtg aattcatact tcagctctct ctgtctggcg ggctgaaacc
     3061 agcccgctaa tctcgaatta gttagcacag gccactgtca tttctgtcgg gcgcggtctg
     3121 ctcattacgg ttttgatttc gtcgcgcagg atttgcgatt tgccgccaaa tatcacctgt
     3181 acaccgccat cacccagttt cactaaaccc gcagccccca actccttcag ccgtttttca
     3241 ttcactttct tatcgtctac cagcgccagc cttaggcgtg ttgcgcaggc atccacatct
     3301 tcgatattgt cgaaaccacc tactgcttcg atgatttggt atggacgttc ttcgcgcgtt
     3361 aatgtggttg caacggcaat ctcttcatcc tcacgccccg gtgttttcag attcaacttg
     3421 cggatcagta cccgaaatac ggtgtagtaa atcgctgcat agcccgcgcc tgccaggaac
     3481 accagatacc acggcgtgcg gtttggcata acgccattca gtacgaaatc aataagaccg
     3541 ccagcaaaag gatgaccaat gtgcacattc aggatgttca tcagcacaaa tgaagtggca
     3601 tataagaaga tgtgaatgat gtagagaacc ggcgctgcaa acaaaaatgc atattccagc
     3661 ggctccgtaa tcccggtaac aaatgtggtc agcgcaccgg agagtaaaag gcctttaatc
     3721 agcgccttac gttccggttt tgcttcctga tacatcgcca acgcggcggc aggtaacgca
     3781 aacatcatga tcgggaaacg acctgtcata aacagccccg ccgtcaacgg agcaccgtcg
     3841 cgcaattggg caaagaacac cggaatatcg cccgtcacca gttgcccgga tttggtcatg
     3901 tagtcgccaa agttgtaata aaacggcacg ttccagatat gatgcaggcc aaaaggaatc
     3961 agcgaacgtt cgccaacggc gtaaataaag gccgcgaagt tcgcgttatg tccactggtc
     4021 accagcgttg aaagcgacgc cagacctttt tgtaccggta tccacagcca cggcaataaa
     4081 tagccgacaa taattgccgc cacagccgta ataatcggta caaagcgttt tccaccgaag
     4141 aaaccaagaa actgaggcaa ttcaatattg tggaagcggg tatagagcag ataaacaaag
     4201 atggcgacaa tcagaccgcc aaacactccc gtttgtaacg tcggtacccc gaggatcatc
     4261 gcataatcac cgttactggc tgcaacggac tccgcagtaa tgccaagttg cgttcccatc
     4321 gtggcattca tgatcatgta actgataatc gctgaaagtg atgaagaacc atcacctgtc
     4381 aggccaatca caataccgac acaaaacagc aaaggaaggt tggcgaaaat caggccgccg
     4441 caattcgcca gcagctcaat gttaaatgaa atcccgatgg ctaaaatcaa acccgccgcc
     4501 ggaagaatgg cgattggtaa catcagagca gaagaaaatt tctgcaactt cttgaagtta
     4561 aacaaggatg ttattttttt gctcattttt attctcctgt agggtatcga gcaaataacg
     4621 gcgacgacta ttccagattg gcgtggcgct taaacagttc actttccggc gcaccacttt
     4681 cagcagccat cttcatcact aatgcatcgg caatcaccag caaagcttgc tcatacaacg
     4741 acgccatcgg ctgtgccgag cggaaaacag aatctttttg atttttggaa ggggcattga
     4801 tttgcacgat gacgtcgaca aactcggtga tagtggcgtg tcgattcgtg gttaacaccg
     4861 ccacagcgcc gccgagctga cgtgcttttt tcgccagcgc caccagttgt gctgtctcac
     4921 cggaagcaga agaaacgata aacagatcgc caggcccaaa gttcggcgta atggtttccg
     4981 tcaggaagta aacctctttt cccatatgca tcaggcgcat ggtgaaggct ttcatagaaa
     5041 aaccggcgcg tcccaggcca tagcagaaaa cgcgctggct ttgctgaata gtgtcgatca
     5101 atttggtgca gtcatcctca cggatagagt ccttaacgaa ggttaattca ttgatgatct
     5161 cagtgatgta cagctttgcg tccatttcac acctcatgaa ataattttct caaacacaaa
     5221 atctcatgaa attttatttc attccaatgc atcagtatga aatgtgaagc tagtcacaag
     5281 aaagat
//
LOCUS       DAHHTG010000211         5219 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.215, whole genome shotgun sequence.
ACCESSION   DAHHTG010000211 DAHHTG010000000
VERSION     DAHHTG010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5219)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5219)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5219
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.215"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            complement(<1..82)
                     /locus_tag="NBI74_004363"
     CDS             complement(<1..82)
                     /locus_tag="NBI74_004363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HCD1934589.1"
                     /translation="MTIEKHERSTKDLVKAAVSGWLGTALE"
     gene            complement(140..1159)
                     /locus_tag="NBI74_004364"
     CDS             complement(140..1159)
                     /locus_tag="NBI74_004364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-dependent oxidoreductase"
                     /protein_id="HCD1934590.1"
                     /translation="MKSILIEKPNQLSIIEREIPTPSAGEVRVKVKLAGICGSDSHIY
                     RGHNPFAKYPRVIGHEFFGVIDAVGEGVESARVGERVAVDPVVSCGHCYPCSIGKPNV
                     CTTLAVLGVHADGGFSEYAVVPAKNAWKIPEAVADQYAVMIEPFTIAANVTGHGQPTE
                     NDTVLVYGAGPIGLTIVQVLKGVYNVKNVIVADRIDERLEKAKESGADWAINNSQTPL
                     GEIFDEKGIQPTLVIDAACHPSILKEAVTLASPAARIVLMGFSSEPSEVIQQGITGKE
                     LSIFSSRLNANKFPVVIDWLSKGLIKPEKLITHTFDFQHVADAISLFEQDQKHCCKVL
                     LTFSE"
     gene            complement(1171..2385)
                     /gene="rspA"
                     /locus_tag="NBI74_004365"
     CDS             complement(1171..2385)
                     /gene="rspA"
                     /locus_tag="NBI74_004365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="starvation-sensing protein RspA"
                     /protein_id="HCD1934591.1"
                     /translation="MKIVKAEVFVTCPGRNFVTLKITTEDGITGLGDATLNGRELSVA
                     SYLQDHLCPQLIGRDAHRIEDIWQFFYKGAYWRRGPVTMSAISAVDMALWDIKAKAAN
                     MPLYQLLGGASREGVMVYCHTTGHSIDEALDDYARHQELGFKAIRVQCGIPGMKTTYG
                     MSKGKGLAYEPATKGQWPEEQLWSTEKYLDFMPKLFDAVRNKFGFNEHLLHDMHHRLT
                     PIEAARFGKSIEDYRMFWMEDPTPAENQECFRLIRQHTVTPIAVGEVFNSIWDCKQLI
                     EEQLIDYIRTTLTHAGGITGMRRIADFASLYQVRTGSHGPSDLSPVCMAAALHFDLWV
                     PNFGVQEYMGYSEQMLEVFPHNWTFDNGYMHPGEKPGLGIEFDEKLAAKYPYEPAYLP
                     VARLEDGTLWNW"
     gene            complement(2591..2917)
                     /locus_tag="NBI74_004366"
     CDS             complement(2591..2917)
                     /locus_tag="NBI74_004366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnfA family protein"
                     /protein_id="HCD1934592.1"
                     /translation="MIKTTLLFFATALCEIIGCFLPWLWLKRNASIWLLLPAGISLAL
                     FVWLLTLHPAASGRVYAAYGGVYVCTALMWLRVVDGVKLTLYDWTGALIALCGMLIIV
                     AGWGRT"
     gene            3052..3393
                     /locus_tag="NBI74_004367"
     CDS             3052..3393
                     /locus_tag="NBI74_004367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1283 family protein"
                     /protein_id="HCD1934593.1"
                     /translation="MKITLSKRIGLLAFLLPCALVLSTTVHAETNKLVIESGDSAQSR
                     QRAAMEKEQWNDTRNLRQKVNKRTEKEWDKADAAFDNRDKCEQSANINAYWEPNTLRC
                     LDRRTGRVIIP"
     gene            3428..3988
                     /gene="speG"
                     /locus_tag="NBI74_004368"
     CDS             3428..3988
                     /gene="speG"
                     /locus_tag="NBI74_004368"
                     /EC_number="2.3.1.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine N1-acetyltransferase"
                     /protein_id="HCD1934594.1"
                     /translation="MPSAHSVKLRPLEREDLRYVHQLDNNASVMRYWFEEPYEAFVEL
                     SDLYDKHIHDQSERRFVVECDGEKAGLVELVEINHVHRRAEFQIIISPEYQGKGLATR
                     AAKLAMDYGFTVLNLYKLYLIVDKENEKAIHIYRKLGFTVEGELMHEFFINGQYRNAI
                     RMCIFQHQYLAEHKTPGQTLLKPTAQ"
     gene            complement(3991..4701)
                     /locus_tag="NBI74_004369"
     CDS             complement(3991..4701)
                     /locus_tag="NBI74_004369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416102.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnfC family lipoprotein"
                     /protein_id="HCD1934595.1"
                     /translation="MKYKLLPCLLAILLTGCDRTEVTLSFTPEMASFSNEFDFDPLRG
                     PVKDFTQTLMDEQGEVTKRVSGTLSEEGCFDSLELLDLENNTLVALVLDANYYRDAET
                     LEKRVRLQGKCQLAELPSAGVSWETDDNGFVIKASSKQMQMEYRYDDQGYPLGKTTKS
                     NDKTLSVSATPSTDPIKKLDYTAVTLLNNQRVGNVKQSCEYDNHANPVDCQLIIVDEG
                     VKPAVERVYTIKNTIDYY"
     gene            4809..5114
                     /locus_tag="NBI74_004370"
     CDS             4809..5114
                     /locus_tag="NBI74_004370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1161 domain-containing protein"
                     /protein_id="HCD1934596.1"
                     /translation="MKHSTYCAALLLALASPTVLAAPGSCERIQSDISQRIINNGVPE
                     SSFTLSIVPNDQVDQPDSQVVGHCANDTHKILYTRTTSGNVSAPAQSTQDGAPAEPQ"
BASE COUNT         1425 a         1310 c         1131 g         1353 t
ORIGIN      
        1 attcaagcgc agtgcccagc catcccgata ctgctgcttt caccaaatcc ttagtgcttc
       61 tttcgtgttt ttctattgtc ataatggtta tctctaaaaa agaggtaaga tgcgtactac
      121 ttattcgccg ttattggtat tattcagaaa aagtgagtaa gactttgcag caatgttttt
      181 gatcctgttc aaataaacta atggcatccg caacatgctg gaaatcaaac gtatgggtaa
      241 ttagtttttc tggtttaatt aaccctttac ttaaccagtc gataacgacc gggaatttat
      301 ttgcatttaa gcgtgaagag aaaatagaga gttcttttcc ggtaattcct tgctgaatca
      361 cttcagacgg ttcactggag aagcccatca atacaatacg tgccgctgga gaagccagcg
      421 ttacggcctc tttcaagatt gaaggatgac aagcggcatc gataactaat gtcggttgga
      481 tgcctttttc atcgaaaatc tcgccaagtg gtgtctggct gttattaatc gcccagtctg
      541 ccccgctctc tttcgctttt tccagtcgtt catcaatgcg atcggcaaca atcacatttt
      601 taacgttata gacgcctttt aatacctgaa cgatcgtcag gccgattgga ccggcaccgt
      661 aaaccagaac ggtatcattt tcagtcggtt gaccatgacc ggtaacgtta gccgcaatgg
      721 taaaaggttc gatcatcacc gcatattgat cggccactgc ttcaggaatt ttccacgcat
      781 tttttgccgg aaccacggca tattcactga aaccaccgtc agcgtgcaca cctaatacag
      841 caagtgtcgt acaaacgttc ggcttaccta tagagcacgg atagcaatgc ccacagctga
      901 ccaccggatc gacagcaacg cgttcaccga ctctggcgct ttccacgcct tcacccactg
      961 catcaatgac gccaaagaat tcatgaccaa tgacgcgcgg atatttcgca aaaggattat
     1021 gcccacggta aatatggcta tctgaaccac aaattccggc aagtttcact tttactcgta
     1081 cttcacccgc tgacggggtg ggtatttcac gttcgataat cgacagttga ttcggttttt
     1141 caattaaaat gcttttcatt accttactcc ttaccagttc cacagcgtgc catcttccag
     1201 acgtgcgact ggcagataag caggttcata gggatatttc gccgccagct tttcatcgaa
     1261 ttcgatgcca agacccggtt tttctcccgg atgcatatag ccgttatcga aagtccagtt
     1321 gtgcgggaag acttcgagca tttgttcgga ataacccatg tattcctgga caccgaaatt
     1381 ggggacccac agatcaaagt gcagcgccgc agccatgcag actggtgaca aatcggaagg
     1441 accgtgtgag ccagtacgta cctgatacag cgaagcaaaa tcggcaatcc gacgcatacc
     1501 ggtaattccg cctgcatgag tcagcgtggt gcggatataa tcgatgagtt gctcttcaat
     1561 cagttgtttg cagtcccaga tgctgttgaa gacttcaccc accgcgatgg gtgtgacggt
     1621 atgttggcga atgagacgga agcattcctg gttttccgca ggcgtcgggt cttccatcca
     1681 gaacatgcga taatcttcaa tgcttttacc aaagcgcgcc gcttcaatag gcgttaagcg
     1741 atggtgcatg tcatgcagca aatgttcatt aaatccaaac ttgttacgta ccgcgtcaaa
     1801 caatttcggc atgaaatcga ggtatttctc cgtcgaccac agctgctctt ccggccactg
     1861 tcctttggtt gcgggttcat aagccagacc tttacctttc gacatgccgt aggtggtttt
     1921 cataccaggg attccgcact gcacgcggat ggctttgaat cccagctcct gatgacgggc
     1981 ataatcatcc agagcttcat caatactgtg accggtggta tggcaataaa ccatcacccc
     2041 ttcacgagac gcgccgccga gtaactggta aagcggcatg ttggcagctt tggctttaat
     2101 atcccacagc gccatatcaa ccgctgaaat ggccgacatc gtaaccggac cgcgacgcca
     2161 gtaagcacct ttatagaaaa actgccagat atcttcgata cggtgtgcat cgcgaccaat
     2221 aagctgcgga caaaggtgat cctgcaaata agaggccacg gaaagctcac gtccatttag
     2281 ggtggcatcc ccaaggcccg taataccgtc ctcagtggtg atttttaatg tgacgaaatt
     2341 acgccccgga caggtaacaa aaacttcagc ctttacgatc ttcatgttcg attccttgca
     2401 tcgcttgtcg tgatgcatga aatctacgca actgaactac taccatacaa gtatgaagat
     2461 caaaaaaagc cggagtgatc acaaaaaaag gcgtatattt gcgccgtgaa tggttgacaa
     2521 aagatgaaat agaatacctt ttgtcagctg acacttcctc gtatcttatt gataaaatgg
     2581 atttatgttc ctacgtgcgc ccccagcccg caacaatgat caacatgccg caaagcgcaa
     2641 ttaacgcccc cgtccagtca taaagagtca gtttcacgcc atccacaacg cgcagccaca
     2701 tcaacgccgt gcaaacataa acgccaccat aagccgcgta aacacgccca ctcgccgcag
     2761 gatgcaacgt taacaaccag acaaacagtg ccagtgaaat ccccgccgga agcaacagcc
     2821 agatactggc gtttcgtttt aaccacaacc agggcagaaa gcatccaata atttcacaca
     2881 gcgcagtagc aaaaaatagt aacgttgttt taatcatctt tgtcacttat tgacatcatg
     2941 tatagttata gggcgacata atatcatcaa tataaacacc ctccttgaac gttttgcgtt
     3001 cgcagtggat ggtgtagaat cagctttaat cattcataca gggaatgaat tatgaaaatc
     3061 actctcagca aacgaatcgg cctgctcgct tttctgctgc cttgcgcact ggtattgagc
     3121 acaactgttc atgccgaaac taacaaactg gtgattgagt ctggcgacag tgcacaaagc
     3181 cgccagcgcg ccgctatgga aaaagagcaa tggaatgaca cgcgtaatct gcgccagaaa
     3241 gtgaataaac gcactgaaaa agagtgggat aaagccgacg ctgcttttga taaccgcgat
     3301 aaatgtgagc aaagcgccaa catcaatgcc tactgggagc caaatacttt gcgctgcctg
     3361 gaccgtcgaa ctggccgcgt tattatcccc taacctgtta ttgatttaag gaatgtaagg
     3421 acacgttatg ccaagcgccc acagtgttaa gctacgcccg ctggagcgtg aagatttacg
     3481 ctatgtacat caactcgaca ataacgccag tgtgatgcgt tactggtttg aggaacccta
     3541 cgaagccttt gttgaactct ctgatctgta tgataagcat attcacgatc agagcgaacg
     3601 gcgctttgtg gtggaatgtg acggcgaaaa agccggtctg gtggagctgg tggaaattaa
     3661 ccatgttcat cgccgcgcag aatttcagat aattatctcc ccggagtatc aggggaaagg
     3721 tctggcaacc cgtgccgcca aattagcaat ggactatggc ttcaccgttc tcaatctcta
     3781 taagctgtat ctgatcgttg ataaagagaa tgaaaaagcg attcacattt accgcaagct
     3841 tggctttacg gttgaaggtg aattgatgca tgagttcttt attaatggtc aatatcgtaa
     3901 tgccattcgc atgtgtatat tccagcatca gtatctggct gagcacaaaa caccgggtca
     3961 gactctcctg aagccgaccg cacaatagta ttaataataa tcgatcgtat ttttgatggt
     4021 ataaacccgt tcgacggcgg gttttactcc ttcatcaaca atgattagct gacagtccac
     4081 cggattggcg tgattgtcat attcacagct ctgttttaca ttaccaaccc gttgattatt
     4141 cagtaaagta acagcagtgt aatctaattt tttgatcggg tccgttgatg gcgtggcgct
     4201 gacagataat gttttatcgt tacttttcgt ggttttaccc agcggatacc cctgatcatc
     4261 atagcgatat tccatctgca tctgtttgct gctggcttta atcacgaaac cattatcatc
     4321 ggtttcccaa ctcaccccgg cagaaggtaa ttctgctagc tggcattttc cctgcaaacg
     4381 aactcttttc tccagcgtct cggcatcacg gtaataattg gcatccagta ccagggcaac
     4441 caaggtatta ttttccagat ccagtaattc gagagaatca aaacagcctt cttccgacaa
     4501 agtccccgaa acacgtttcg tcacttcacc ttgctcatcc attaatgtct gagtgaaatc
     4561 ttttacggga ccacgcagcg gatcaaaatc gaattcatta gagaaactgg ccatctccgg
     4621 ggtaaatgaa agtgttacct ctgtgcggtc acatcctgtg agtaatatcg cgagtaagca
     4681 tggcagtaat ttgtatttca caacagtcac ctgagagtag agatgattct caatcatagt
     4741 agcaaataca gtactttaca cgttaaatgc tatgcttaaa gaagttatct tcgcgtaagg
     4801 agcttatgat gaaacactca acctactgtg ccgcgcttct gctcgccctc gcctcaccca
     4861 cggtactcgc cgcgccggga tcctgtgagc gcatacagag cgatatatca cagcgcatta
     4921 tcaataatgg cgtacccgaa agcagcttca cgttaagtat tgtacccaat gaccaggttg
     4981 atcagcctga ttcccaggtc gtcgggcatt gcgctaatga tacgcataaa attctctaca
     5041 cccgtaccac cagcggtaac gtctctgctc ccgcgcagtc tacccaggat ggcgcgcctg
     5101 ccgaaccgca gtaacacatt atcgactgaa cgccggatgc gacacatccg gcgatttgaa
     5161 cattcaacat aaccccacct tattactcat acccttctat tgatatggat taataatta
//
LOCUS       DAHHTG010000212         5155 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.216, whole genome shotgun sequence.
ACCESSION   DAHHTG010000212 DAHHTG010000000
VERSION     DAHHTG010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5155)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5155)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5155
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.216"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            <1..1742
                     /locus_tag="NBI74_004371"
     CDS             <1..1742
                     /locus_tag="NBI74_004371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016159766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aryl-sulfate sulfotransferase"
                     /protein_id="HCD1934597.1"
                     /translation="LSASGVMAAGFKPAPPAGQLGAIIVDPYGNAPLTALVDLDSHVI
                     SDVKVTVHGKGEKGVDISYKVGNESLKTYDGVPIFGLYQKFANKVTVEWKENGKAMKD
                     DYVVQTSAIVNNYMDNRSISDLQQTKVVKVAPGFEDRLYLVNTHTFTAQGSDLHWHGE
                     KDKNAGILDAGPATGALPFDIAPFTFIVDTEGEYRWWLDQNTFYDGRDRNINKRGYLM
                     GIRETPRGTFTAVQGQHWYEFDMMGQVLEDHKLPRGFADATHESIETPNGTVLLRVGK
                     SNYRRDDGVHVTTIRDHILEVDKSGRVVDVWDLTKILDPKRDALLGALDAGAVCVNVD
                     LAHAGQQAKLEPDTPFGDALGVGPGRNWAHVNSIAYDAKDDSIILSSRHQGVVKIGRD
                     KQVKWILAPSKGWEKPLASKLLKPVDANGKPITCNENGLCENSDFDFTYTQHTAWISS
                     KGTLTIFDNGDGRHLEQPALPTMKYSRFVEYKIDEKKGTVQQVWEYGKERGYDFYSPI
                     TSIIEYQADRNTMFGFGGSIHLFDVGQPTIGKLNEIDYKTKEVKVEIDVLSDKPNQTH
                     YRALLVRPQQMFK"
     gene            1760..2428
                     /locus_tag="NBI74_004372"
     CDS             1760..2428
                     /locus_tag="NBI74_004372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA/DsbL"
                     /protein_id="HCD1934598.1"
                     /translation="MSKLGISSLFKTILLTAALAVSFTASAFTEGTDYMVLEKPIPNA
                     DKTLIKVFSYACPFCYKYDKAVTGPVSEKVKDIVAFTPFHLETKGEYGKQASEVFAVL
                     INKDKAAGISLFDANSQFKKAKFAYYAAYHDKKERWSDGKDPAAFIKTGLDAAGMSQA
                     DFEAALKEPAVQETLEKWKASYDVAKIQGVPAYVVNGKYLIYTKSIKSIDAMADLIRE
                     LASK"
     gene            2443..3114
                     /gene="dsbI"
                     /locus_tag="NBI74_004373"
     CDS             2443..3114
                     /gene="dsbI"
                     /locus_tag="NBI74_004373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000511507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide oxidoreductase DsbI"
                     /protein_id="HCD1934599.1"
                     /translation="MGIKGMWKDLRTSPVDTLVRWQEQRLLWLLMAVAMGTLIILAHS
                     FFQIYLYMAPCEQCVYIRYAMFVMVIGGLVAAINPKNIILKLIGCVMAFYGSILGLKF
                     SLKLNDIHHAVHNPDPDSLFGVQGCSTDPTFPFNLPLAQWAPNWFKPTGDCGYDAPIV
                     PDGVTLSSTQQWFVEMYQQSEGWYLLPPWHFMNMAQACMLAFGMCLVLLVIMSGAWAL
                     KIIRG"
     gene            complement(3160..3948)
                     /gene="ygiD"
                     /locus_tag="NBI74_004374"
     CDS             complement(3160..3948)
                     /gene="ygiD"
                     /locus_tag="NBI74_004374"
                     /EC_number="1.13.11.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001356237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4,5-DOPA dioxygenase extradiol"
                     /protein_id="HCD1934600.1"
                     /translation="MSSTRMPALFLGHGSPMNVLEDNLYTRSWQKLGMTLPRPQAIVV
                     VSAHWFTRGTGVTAMETPPTIHDFGGFPQALYDTHYPAPGSPALAQHLVELLAPVPVT
                     LDKEAWGFDHGSWGVLIKMYPDADIPMVQLSIDSSKPAAWHFEMGRKLAALRDEGIML
                     VASGNVVHNLRTVKWHGDSSPYPWAMSFNEYVKANLTWQGPVEQHPLVNYLDHEGGAL
                     SNPTPEHYLPLLYVLGAWDGQEPITIPVDGIEMGSLSMLSVQIG"
     gene            4091..4864
                     /gene="zupT"
                     /locus_tag="NBI74_004375"
     CDS             4091..4864
                     /gene="zupT"
                     /locus_tag="NBI74_004375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HCD1934601.1"
                     /translation="MSVPLILTILAGAATFIGAFLGVLGQKPSNRLLAFSLGFAAGIM
                     LLISLMEMLPAALAAEGMSPVLGYGMFIFGLLGYFGLDRMLPHAHPQDLMQKSVQPLP
                     KSIKRTAILLTLGISLHNFPEGIATFVTASSNLELGFGIALAVALHNIPEGLAVAGPV
                     YAATGSKRTAILWAGISGLAEILGGVLAWLILGSMISPVVMAAIMAAVAGIMVALSVD
                     ELMPLAKEIDPNNNPSYGVLCGMSVMGFSLVLLQTAGIG"
     gene            complement(4926..5096)
                     /locus_tag="NBI74_004376"
     CDS             complement(4926..5096)
                     /locus_tag="NBI74_004376"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1934602.1"
                     /translation="MFIAWYWIVLIVLLAGGYFLHLKRYCKAFRHDRDALLAARSKLD
                     KRSDKEVFEKHQ"
BASE COUNT         1334 a         1269 c         1258 g         1294 t
ORIGIN      
        1 gtttgtcagc atcgggggtg atggctgcgg gttttaaacc agcaccgcct gccgggcagc
       61 ttggggcgat tatcgtcgat ccctacggta atgcgccctt aaccgcgctg gttgatttag
      121 acagccatgt tatttctgac gtcaaagtta ccgtccacgg gaagggcgaa aaaggcgttg
      181 acatcagcta taaagtgggc aacgagtcac tgaaaactta cgatggtgta ccgatttttg
      241 gtctttacca gaagtttgcg aacaaagtga ccgttgagtg gaaagaaaac ggtaaggcca
      301 tgaaagatga ttacgtggtg cagacttccg ccatcgtcaa caattacatg gataaccgct
      361 ccatctccga cctacagcaa accaaagtcg ttaaagtcgc gccaggtttt gaagatcgcc
      421 tctatctggt taatacccac acctttaccg cccaaggttc cgatctccac tggcatggtg
      481 agaaagataa aaatgccggt atccttgatg cgggtccggc aactggcgca ctcccttttg
      541 atatcgcgcc attcaccttt atcgtcgata cggaaggcga ataccgctgg tggttggatc
      601 aaaacacctt ctacgatggt cgtgaccgca acattaacaa acgtggttat ctgatgggta
      661 tccgtgaaac gccacgcggc acctttaccg ctgtacaagg tcagcactgg tacgagttcg
      721 acatgatggg gcaggtgctc gaagatcaca aactaccgcg cggatttgct gacgccactc
      781 atgagtccat tgagacgcca aatggcacgg tactgttgcg cgtaggtaag agtaactatc
      841 gtcgcgatga cggcgtacac gtcaccacca ttcgtgacca tatcctcgaa gtcgataaat
      901 ctggtcgcgt tgtcgatgta tgggatctga ccaagatcct cgatccgaaa cgcgatgcac
      961 tgctcggcgc gctggatgca ggtgcagttt gcgttaacgt tgaccttgcc catgcaggac
     1021 aacaggcaaa actggaacca gatacaccgt tcggcgacgc gctgggtgta gggccaggcc
     1081 gcaactgggc gcacgtgaac tctatcgcct atgacgcaaa agatgactct atcatcctct
     1141 cctctcgcca ccagggtgtt gtgaagattg gtcgtgataa gcaagtgaaa tggatccttg
     1201 cgccctctaa aggttgggaa aaaccgctgg ccagcaagct gctgaaaccg gttgatgcta
     1261 acggtaagcc aattacctgt aacgaaaatg gcctgtgcga aaactcagac ttcgacttta
     1321 cctacaccca gcataccgcc tggatttcca gcaaaggaac gctcaccatt tttgataatg
     1381 gcgatgggcg tcatctggaa caacctgcct taccaaccat gaaatactcc cgctttgtgg
     1441 aatataagat tgatgagaag aaaggcaccg ttcagcaagt gtgggaatac ggtaaagaac
     1501 gtggctacga tttctatagc ccgattacct ccatcattga atatcaagcc gaccgtaaca
     1561 ccatgtttgg cttcggtggt tctattcatt tgttcgatgt cgggcagcca accatcggta
     1621 agttgaacga aatcgattac aaaaccaaag aagtgaaagt ggaaatcgac gtgctgtcag
     1681 ataaacccaa tcagactcac tatcgtgcac tgttagtccg tccacaacag atgttcaaat
     1741 aattaagaag ggaataaaaa tgtcgaaatt agggatctca tcactattta aaaccatact
     1801 gcttaccgca gctttggctg tttcatttac cgcttctgca tttaccgaag gcacggatta
     1861 catggttctg gaaaaaccta ttccgaatgc cgacaaaacg ctgattaagg tatttagcta
     1921 cgcctgcccg ttctgttaca agtacgacaa agcggttacc ggtccggtat cggaaaaagt
     1981 gaaagatatc gttgctttca cgccgttcca tctggaaacc aaaggtgaat acggcaaaca
     2041 agcaagcgaa gtctttgcgg tcttgatcaa taaagataaa gcagcaggca tttccttatt
     2101 tgatgctaac tctcagttta agaaagccaa gtttgcctac tacgcggctt atcacgacaa
     2161 aaaagaacgc tggtctgacg gtaaagatcc cgctgccttt atcaaaactg gtctggatgc
     2221 cgcaggtatg agtcaggcgg attttgaagc ggcactgaaa gaaccagcag ttcaagaaac
     2281 actggaaaaa tggaaagcct cttatgatgt cgccaaaatc cagggggttc cagcctatgt
     2341 cgttaatggt aagtacttga tctacaccaa gagcatcaaa tccatcgacg cgatggcaga
     2401 ccttatccgt gagttagcaa gcaaataaca gggaaacagg ctatgggtat taagggaatg
     2461 tggaaagatc ttcgcacatc accagttgac acactggtca gatggcagga gcaacgcttg
     2521 ttgtggttgt taatggccgt tgcgatgggc acgttaatta tcctggcgca ctctttcttc
     2581 cagatctatc tctacatggc accctgtgag caatgtgtat acattcgcta cgccatgttt
     2641 gtgatggtga ttggaggatt ggttgcagcg atcaatccaa agaacatcat cctcaagcta
     2701 attggctgcg tgatggcatt ttacggcagc attttgggac tgaagttttc gctcaaactg
     2761 aacgatatcc accatgcagt acataatccg gatcccgatt cactgtttgg cgtacagggt
     2821 tgctctaccg atcccacttt tccctttaac ctgccgctgg cacagtgggc accaaattgg
     2881 ttcaagccta ccggggattg tggctatgac gcaccaatcg ttcctgatgg cgtcacactg
     2941 agcagcacgc aacagtggtt tgtggaaatg tatcagcaat ctgaaggctg gtatctgcta
     3001 ccaccatggc attttatgaa tatggctcag gcttgtatgc tggcttttgg tatgtgtctg
     3061 gtgctgctgg tcattatgag tggcgcatgg gcgcttaaaa tcatcagagg ctaactcagc
     3121 aacacccgaa gccagtcatt ctggcttcgg gttcttttat taacctatct gcaccgacag
     3181 catacttaaa ctgcccattt ctataccatc gaccggaatg gtaattggct cctgcccatc
     3241 ccacgcccct aagacataca acaacggcag atagtgctct ggcgttgggt tcgataacgc
     3301 gccaccttca tggtcgagat aattcaccag aggatgttgt tccaccggcc cttgccacgt
     3361 cagattcgct ttcacatact cattaaacga catcgcccac ggatacggtg aactatcacc
     3421 gtgccacttc actgtacgca ggttatgcac cacgttgcca ctggcgacca acattatccc
     3481 ttcatcacgc agcgccgcga gtttgcgccc catttcgaaa tgccaggcgg caggtttgct
     3541 actgtcgata ctcaactgca ccatcgggat atcggcgtca ggatacatct taatcagtac
     3601 gccccacgag ccgtggtcga agccccaggc ttctttatcc agcgtcacag ggaccggcgc
     3661 taacagctca accagatgct gtgccagcgc aggtgaaccc ggagcaggat aatgcgtatc
     3721 gtacagcgcc tgcgggaagc caccaaagtc atgaatcgtg ggcggcgtct ccatcgcggt
     3781 cactcctgtt ccacgggtaa accaatgagc cgaaaccacc acaatcgctt gcgggcgtgg
     3841 caatgtcatc cccaacttct gccagctgcg ggtatacaaa ttatcttcca gcacgttcat
     3901 cggactaccg tgacctaaaa acaatgctgg catacgtgtt gaagacatga tgatatcctt
     3961 aactaaaggt gtcattttga tatcctcaca atacgcttgt tcggcggagt aagaacccgg
     4021 ataacaatga tgatgatcat cagttatttt gacgatctgc ctgaaggtga agatttataa
     4081 ggagttgtcg atgtcagtac ctctcattct gaccatactg gcgggggcag ccacgtttat
     4141 tggcgcgttt ctcggcgttc tcgggcaaaa accctcgaac cgcttactgg cgttttcgct
     4201 aggatttgcg gcggggatca tgttgctcat ctcattaatg gaaatgcttc ctgccgcatt
     4261 agccgccgaa ggaatgtcac ctgtgttggg ttatggaatg tttatcttcg gtctgcttgg
     4321 ctattttggt ctggaccgca tgttgccaca tgcgcatccg caggatttaa tgcaaaaatc
     4381 ggtgcagccg ttgccaaaat cgatcaagcg cacagccatt ctgctcactc tcggtatcag
     4441 tctgcataac ttcccggaag ggattgccac ctttgtcacg gcgagcagca acctggagct
     4501 gggatttggt atcgccctgg ccgtagcgtt gcacaatatt cctgaaggtc tggcggttgc
     4561 aggcccggtt tacgcggcaa cggggtcaaa acgtaccgca attctgtggg cggggatttc
     4621 cggactggct gaaattctcg gtggtgtgct ggcatggtta attcttggca gcatgatttc
     4681 tccggtggtc atggcggcaa tcatggcggc ggttgccggt attatggtgg cgctctcggt
     4741 tgatgaatta atgccgctcg ccaaagagat tgaccccaat aataacccca gttatggcgt
     4801 gctatgtgga atgtcagtga tgggattcag tttagtgctg ctacaaacgg caggaattgg
     4861 ttaaagatga taatgacaga tgttgcccat tgctaaaggc aacatctgta tcgttattaa
     4921 aggtattatt gatgcttttc aaacacttct ttatcggagc gtttatctaa tttacttcgg
     4981 gcagctaaca gcgcatctct gtcatggcga aaagctttac agtaacgttt aagatgaaga
     5041 aaatagccac cagctagtag aacaattaat acaatccagt accaggcaat aaacatcctt
     5101 ttcaccttca aaatttaaca ttactaaaca tttagcgtta aaatttcaca tatcc
//
LOCUS       DAHHTG010000213         4911 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.217, whole genome shotgun sequence.
ACCESSION   DAHHTG010000213 DAHHTG010000000
VERSION     DAHHTG010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4911)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4911)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4911
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.217"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            complement(<1..68)
                     /locus_tag="NBI74_004377"
     CDS             complement(<1..68)
                     /locus_tag="NBI74_004377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCD1934603.1"
                     /translation="MVTVAELQALRQARLDLLTGKRV"
     gene            complement(52..1980)
                     /locus_tag="NBI74_004378"
     CDS             complement(52..1980)
                     /locus_tag="NBI74_004378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HCD1934604.1"
                     /translation="MISDAQKAANAAGAIATGLLSLIIPVPLTTVQWANKHYYLPKES
                     SYTPGRWETLPFQVGIMNCMGNDLIRTVNLIKSARVGYTKMLLGVEAYFIEHKSRNSL
                     LFQPTDSAAEDFMKSHVEPTIRDVPALLELAPWFGRKHRDNTLTLKRFSSGVGFWCLG
                     GAAAKNYREKSVDVVCYDELSSFEPDVEKEGSPTLLGDKRIEGSVWPKSIRGSTPKIK
                     GSCQIEKAANESAHFMRFYVPCPHCGEEQYLKFGDDASPFGLKWEKNKPESVFYLCEH
                     HGCVIHQSELDQSNGRWICENTGMWTRDGLMFFSARGDEIPPPRSITFHIWTAYSPFT
                     TWVQIVYDWLDALKDPNGLKTFVNTTLGETWEEAVGEKLDHQVLMDKVVRYTAAVPAR
                     VVYLTAGIDSQRNRFEMYVWGWAPGEEAFLVDKIIIMGRPDEEETLLRVDAAINKKYR
                     HADGTEMTISRVCWDIGGIDGEIVYQRSKKHGVFRVLPVKGASVYGKPVITMPKTRNQ
                     RGVYLCEVGTDTAKEILYARMKADPTPVDEATSYAIRFPDDPDIFSQTEAQQLVAEEL
                     VEKWEKGKMRLLWDNKKRRNEALDCLVYAYAALRVSVQRWQLDLAVLAKSREEETTRP
                     TLKELAAKLSGGVNGYSR"
     gene            complement(1952..2500)
                     /locus_tag="NBI74_004379"
     CDS             complement(1952..2500)
                     /locus_tag="NBI74_004379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000867575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HCD1934605.1"
                     /translation="MKVNKKRLAEIFNVDPRTIERWQSQGLPCASKGSKGIESVFDTA
                     MAIQWYAQRETDIENEKLRKELDDLRAAAESDLQPGTIDYERYRLTKAQADAQELKNA
                     REDGVVLETELFTFILQRVAQEISGILVRVPLTLQRKYPDISPSHLDVVKTEIAKASN
                     VAAKAGENVGGWIDDFRRAEGS"
     gene            complement(2582..2860)
                     /locus_tag="NBI74_004380"
     CDS             complement(2582..2860)
                     /locus_tag="NBI74_004380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1934606.1"
                     /translation="MHTGFLPRLLFAGLSLMLASPVRGWKKQDVYSGLSDIEFIANKP
                     ERLRVLSGDPTGYGAATSRFFTIYENFSGKIVSVLLEYNFLFFLILHS"
     gene            complement(3092..3466)
                     /locus_tag="NBI74_004381"
     CDS             complement(3092..3466)
                     /locus_tag="NBI74_004381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000084844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1934607.1"
                     /translation="MSQYAQAALNAYQLVAHNSMSPRDAWEAAVAEVTESESARKKGC
                     PRATFLALADSGYLKNVKQHHGEKKIGKLYQRAIEVANLILDLPGISKAELVDKTCYK
                     DRQGSYDIALTLAQHGLLQRPQ"
     gene            complement(3505..3948)
                     /locus_tag="NBI74_004382"
     CDS             complement(3505..3948)
                     /locus_tag="NBI74_004382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001298489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HCD1934608.1"
                     /translation="MSRVTAIISALVICIIVCLSWAVNHYRDNAIAYKEQRDKAASTI
                     ADMQKRQRDVAELDARYTKELADANATIESLRADVFAGRKWLRVKAVCPDMHKTTAAS
                     GVDDGASPRLTDTAQRDYFVLRERIETVTNQLNGLQEYVRSQCSY"
     gene            complement(3945..4442)
                     /gene="rrrD"
                     /locus_tag="NBI74_004383"
     CDS             complement(3945..4442)
                     /gene="rrrD"
                     /locus_tag="NBI74_004383"
                     /EC_number="3.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme RrrD"
                     /protein_id="HCD1934609.1"
                     /translation="MSPSLRKAIAAAIGGGAVAIASVLITGPSGDDGLEGVSYIPYKD
                     IVGVWTVCHGHTGKDIMLGKTYTEAECKALLNKDLATVARQINPYIKVDIPETTRGAL
                     YSFVYNVGAGNFRTSTLLRKINQGDIKGACDQLRRWTYAGGKQWKGLMTRREIEREVC
                     LWGQQ"
     gene            complement(4442..4657)
                     /gene="essD"
                     /locus_tag="NBI74_004384"
     CDS             complement(4442..4657)
                     /gene="essD"
                     /locus_tag="NBI74_004384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage lysis protein EssD"
                     /protein_id="HCD1934610.1"
                     /translation="MKSMDKLTTGVAYGTSAGSAGYWFLQLLDKVTPSQWAAIGVLGS
                     LVFGLLTYLTNLYFKIKEDKRKAARGE"
BASE COUNT         1239 a         1318 c         1110 g         1244 t
ORIGIN      
        1 acccgtttac cggttaataa atcaaggcgc gcctgacgca gcgcctgcag ttcagcgact
       61 gtaaccattc actcctccgg acagcttcgc tgccagttct ttaagggttg gccgggtcgt
      121 ctcttcttcc cgggattttg ccagtacagc cagatcaagc tgccagcgtt gcacggacac
      181 acgtaatgcc gcgtaggcat acaccaggca gtccagcgct tcgttacgcc gctttttgtt
      241 atcccacagc agacgcatct ttcctttttc ccacttctcc acaagctctt ccgcgaccag
      301 ttgctgcgcc tctgtctgcg aaaaaatatc cggatcatca ggaaaacgga tggcatacga
      361 cgtggcttca tccacaggcg tgggatcggc tttcatacgg gcatagagaa tttcttttgc
      421 ggtgtccgtc cccacttcac acagatacac gccccgctga ttgcgggttt ttggcatggt
      481 gatcaccggc ttgccataga cagatgcgcc ttttaccggc agcacccgga aaacaccgtg
      541 tttttttgac ctctgataaa cgatttcgcc atcgatcccc ccgatgtccc agcagacacg
      601 ggaaatagtc atttcggttc cgtctgcatg gcggtatttt ttgttgatcg ccgcatccac
      661 acgtaacagc gtctcttcct catcgggacg ccccataatg atgattttat ccaccagaaa
      721 ggcttcctct cccggagccc atccccagac atacatctca aaacggtttc gctgcgagtc
      781 aatgcccgcc gtcagataaa ccacccgggc aggcaccgcc gccgtgtaac gcacaacctt
      841 atccatcagc acctggtgat cgagtttttc gcccacagcc tcttcccagg tctcgcccag
      901 cgtggtgttc acaaaggttt tcaggccgtt gggatctttc agtgcatcca gccagtcata
      961 gacaatctgt acccaggtgg tgaacggact gtacgccgtc cagatatgga aagtgatgga
     1021 gcgcggcggc ggaatttcat caccccgggc gctgaaaaac atcaggccgt cacgggtcca
     1081 catgcccgtg ttttcacaga tccaccgccc gttactctgg tcaagctcag actgatggat
     1141 cacacagcca tgatgctcac aaaggtagaa aacactttct ggcttattct tctcccactt
     1201 aagaccgaaa ggcgaggcat catcgccaaa tttcagatac tgctcctccc cacagtgcgg
     1261 acagggcaca taaaaacgca tgaagtgtgc cgactcgtta gcggcttttt cgatctggca
     1321 ggagcctttg attttaggcg tcgagccgcg aatggatttt ggccatacag agccctcaat
     1381 acgtttatcc cccagcaggg ttggcgaacc ctctttttca acatccggtt cgaacgagga
     1441 aagctcgtca taacagacca catccacgga tttttcacgg tagttttttg ctgccgcacc
     1501 acccagacac cagaacccca caccggagga aaaacgcttc agggtgagcg tattatcgcg
     1561 gtgttttctt ccgaaccatg gagccagctc cagcaatgca ggaacatccc ttatcgttgg
     1621 ctcaacatga gatttcataa aatcttcagc agctgagtcc gtgggctgaa aaagaaggct
     1681 gttgcgtgat ttatgctcaa taaaataagc ctccactccc agcaacatct ttgtataacc
     1741 aacacgggca gatttaatca ggttaaccgt gcgaatcaga tcgttgccca tacagttcat
     1801 gatgccaacc tgaaacggca gtgtttccca ccgccccggg gtataagacg actctttagg
     1861 aaggtaataa tgtttattgg cccactgaac tgtcgtcagt ggaacaggaa taatgagaga
     1921 taaaagccct gtagctatcg caccggctgc attagctgcc ttctgcgcgt ctgaaatcat
     1981 cgatccaccc gcccacgttt tcaccggcct tagctgcaac attggaggct ttcgcgattt
     2041 cagttttcac cacatcaagg tgtgatggtg aaatgtccgg atatttacgc tgtaatgtca
     2101 acggcacacg cacaagtatc cccgaaatct cctgtgccac acgttgcaga atgaaggtaa
     2161 acagttcagt ttccagcact actccgtctt cacgggcatt tttcagttcc tgcgcatctg
     2221 cctgcgcttt tgtgagccgg tagcgttcat agtcaatggt gccgggttgt aaatctgact
     2281 ccgctgccgc acgcaaatcg tccagttctt tgcggagctt ttcgttttcg atatcagttt
     2341 ccctctgcgc ataccactga attgccatgg cagtatcaaa tacagattca atgcccttac
     2401 tacctttgga ggcgcaaggg agtccctgag actgccagcg ttcaatcgtc cgcgggtcca
     2461 cgttgaaaat ttcggcaagc ctctttttat taaccttcat gaaaacaacc cattatcaaa
     2521 tacaaggccc gacatgaaaa cgccagaaaa aggcattttc ggacactttc atgtcggacc
     2581 tttacgaatg caatattaaa aaaaacaaaa agttatattc gagaagtacc gacacgattt
     2641 tccctgaaaa attttcataa atagtgaaaa accgcgaggt cgccgccccg taacctgtcg
     2701 gatcgccgga aaggacccgc aggcgttctg gtttattcgc aatgaattca atatctgaca
     2761 gacctgaata cacgtcctgt ttcttccacc cccgcacagg actggcgagc atgagggaca
     2821 aacccgcgaa taataaacgc ggtaaaaacc cggtgtgcat cgtttttgat tattcccgca
     2881 cactcgcgca gaaggaattc cccgtcgggc tacggtcatg gttaatgcag gaatacggcg
     2941 acgatacagc gcataatgtg tcaggcttga atacctttat cgaatcccgg taacgaattc
     3001 tcctgacgtt ccagtcaggt gatgatatgc atggtgagat tcagatatga aaaagccccg
     3061 ctaaaagcga ggccataaat cacttaatat cttattgagg acgctggagt aatccgtgct
     3121 gagcgagagt tagagcaatg tcataagacc cttgcctgtc tttatagcaa gttttatcaa
     3181 ctagctcagc tttgctaatt ccgggtaaat caagaatcag attcgcaact tcaattgccc
     3241 tttggtacaa cttaccgatc tttttctccc catgatgttg ttttacattc ttcagataac
     3301 cgctatccgc cagagcgaga aacgttgccc ttgggcatcc cttctttctt gccgattcgc
     3361 tttctgtaac ctcagcgaca gcagcctccc atgcatcacg aggagacatt gagttatgag
     3421 caaccaattg ataagcattt aaagcggctt gagcgtattg agacataatg catccatata
     3481 caaaaatctt atgataagac ttttctaata tgaacactgt gatctcacat actcttgcag
     3541 accattcaat tggttagtta cggtttcgat gcgctctctg agaacgaaat aatcccgttg
     3601 agcggtgtca gtaagtctgg ggctggcacc atcatccacg ccggaggcgg cggtggtttt
     3661 atgcatgtcc ggacagactg ctttgacgcg cagccactta cgaccagcaa aaacatcagc
     3721 acgtagactt tcgatagtcg cgttagcatc agcaagctcc tttgtgtatc tggcgtcgag
     3781 ttctgctaca tcacgttgac gcttctgcat gtcagcgata gtagatgcgg ctttgtcgcg
     3841 ctgctctttg taggcgatgg cgttatcacg gtaatgatta acagcccatg acaggcagac
     3901 gatgatgcag ataaccagag cggagataat cgcggttact ctgctcattg ttgcccccac
     3961 aaacagactt cacgctcaat ctcacggcga gtcatcagcc ctttccattg cttaccgcca
     4021 gcgtatgtcc agcgccgtag ctgatcacat gcgcctttga tatcgccctg gtttattttg
     4081 cgaagaagcg tcgatgttct gaaattgcca gcgcccacgt tgtaaacgaa cgagtaaaga
     4141 gcgccgcgcg ttgtttccgg tatatcgact ttgatgtacg ggttaatttg tctggcgaca
     4201 gtggcaaggt ctttattcag gagggctttg cattctgctt cggtatacgt tttaccgagc
     4261 atgatgtctt ttccggtgtg tccgtgacat acagtccata cgccaacgat atctttgtat
     4321 ggtatgtagc tgacaccttc caggccatcg tcaccactcg gaccagtgat gagcacagac
     4381 gctatggcaa cagccccacc accaatagca gcagcaatag ccttgcgtaa tgatggcgac
     4441 attattcacc tctcgcagcc ttacgcttat cttctttaat cttgaaataa aggtttgtca
     4501 gatacgtcag caagccaaat accagactac ccagcacacc tattgccgcc cactgtgagg
     4561 gcgtgacttt atcgagcaac tgtaaaaacc agtacccggc actacctgct gaggtgccat
     4621 aggcgacacc cgttgttaac ttatccatgg atttcataac cccacctcgc agacaaagcg
     4681 ggtgtaaatt aaggggatac tacgtatcgc aataaaggca gaaacgtaac agattcggag
     4741 tcagtgaata actcaggtat tgggttatca gctaatatcg agactcaaaa aatggaaaaa
     4801 cccgctcgac ggcgggttta agctgtgtga cgaagtaacc actcttaaca gcataaccaa
     4861 ttttttacgt acgtaaacta ctaaatgata tttgtgagaa tgccaccgag t
//
LOCUS       DAHHTG010000214         4873 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.218, whole genome shotgun sequence.
ACCESSION   DAHHTG010000214 DAHHTG010000000
VERSION     DAHHTG010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4873)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4873)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4873
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.218"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            21..548
                     /locus_tag="NBI74_004385"
     CDS             21..548
                     /locus_tag="NBI74_004385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000290782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HCD1934611.1"
                     /translation="MAVRGINKVILVGRLGKDPEVRYIPNGGAVANLQVATSESWRDK
                     QTGEIREQTEWHRVVLFGKLAEVAGEYLRKGAQVYIEGQLRTRSWEDNGITRYVTEIL
                     VKTTGTMQMLGRAAGTQTQPEEAQQFSGQPQPESQPEPKKGGAKTKGRGRKAAQPEPQ
                     QPSEPAYDFDDDIPF"
     gene            604..837
                     /locus_tag="NBI74_004386"
     CDS             604..837
                     /locus_tag="NBI74_004386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 domain-containing protein"
                     /protein_id="HCD1934612.1"
                     /translation="MSEYFRILQGLPDGSFTREQAEAVAAQYRNVFIEDDQGTHFRLV
                     VRNNSGLVWRTWNFEDGAGYWMNHVIRNFGILK"
     gene            896..2854
                     /locus_tag="NBI74_004387"
     CDS             896..2854
                     /locus_tag="NBI74_004387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HCD1934613.1"
                     /translation="MPVTKCEPETTRKASRKYAKTQETVLSALLAQTEEVSVPLASLI
                     KSPLNVRTVPYSAESVSELAESIKGVGLLQNLVVHTLPGDRYGVAAGGRRLAALNMLA
                     ERDIIPADWPVRVKIIPQELATAASMTENGHRRDMHPAEQIAGFRAMAQEGKTPAHIG
                     DLLGYSPRHVQRMLKLADLAPVILDALAEDRITTEHCQALALENDTARQVQVFEAACQ
                     SGWGGKPEVQTIRRLVTESEVAVAGNSKFRFVGADAFSPDELRTDLFSDDGDGYVDRV
                     ALDAALLEKLQAVAEHLREAEGWEWCAGRMEPVGECREDAGTYRCLPEPEAVLTEAED
                     ERLNELMTRYDALENQCEESDLLEAEMKLMRCMAKVRAWTPEIRAGSGVVVSWRYGNV
                     CVQRGVQLRSEDDATDDADRTEQVQEKASVEEISLPLLTKMSSERTLAVQAALMQQPD
                     KSLALLAWTLCLNVFGSGAYSKPAQISLECEHYSLTSDAPSGKEGAAFMALMAEKSRL
                     AALLPEGWSRDMTTFLSLSQEVLLSLLSFCTACSLNGVQTRECGHTSRSPLDSLESAI
                     GFHMRDWWQPTKANFFGHLKKPQIIAALNEAGLSGAARDAEKMKKGDAAEHAEHHMKD
                     NRWVPGWMCAPHPQTDATERTDNLADAA"
     gene            2909..3343
                     /gene="psiB"
                     /locus_tag="NBI74_004388"
     CDS             2909..3343
                     /gene="psiB"
                     /locus_tag="NBI74_004388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000845953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugation system SOS inhibitor PsiB"
                     /protein_id="HCD1934614.1"
                     /translation="MKTELTLNALQSMNAQEYEDIRAAGSDMRRNLTHEVMREVDAPA
                     NWMMNGEYGSEFGGFFPVQVRFTPAHERFHLALCSPGDVSQVWVLVLVNAGGEPFAVV
                     QVQRRFAPEAVSHSLALAASLDAQGYSVNDIIHILMAEGGQA"
     gene            3340..4059
                     /locus_tag="NBI74_004389"
     CDS             3340..4059
                     /locus_tag="NBI74_004389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid SOS inhibition protein A"
                     /protein_id="HCD1934615.1"
                     /translation="MSARSQALVPLSTEQQAAWRAVAETEKRRHQGNTLAEYPYAGAF
                     FRCLNGSRRISLSDLRFFMPSLTAEELHGNRLQWLYAIDVLIETQGEVCLLPLPGDAA
                     ERLFPSVRFRVRERSRHKSALVMQKYSRQQAREAEQKARAYQALVAQAEIELAFHSPE
                     TVGSWHARWSDRVAEHDLETLFWQWGERFPSLAGMERWQWQDMPFWQVIAEASLAARE
                     AGHAVREMERWMVPNKLREAA"
     gene            complement(4071..4259)
                     /locus_tag="NBI74_004390"
     CDS             complement(4071..4259)
                     /locus_tag="NBI74_004390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="protein sok"
                     /protein_id="HCD1934616.1"
                     /translation="MKINLLRGYLLSATQHGNKPPSRHEAESLKRRAHHSPYTCTLTT
                     LTFPENNPLIQTVHGKSV"
     gene            4339..4497
                     /locus_tag="NBI74_004391"
     CDS             4339..4497
                     /locus_tag="NBI74_004391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Hok family toxin"
                     /protein_id="HCD1934617.1"
                     /translation="MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAA
                     FMAYESGK"
BASE COUNT         1082 a         1289 c         1520 g          982 t
ORIGIN      
        1 tcaacagaag gagacacatc atggcagttc gtggcattaa caaggtcatt cttgtcggtc
       61 gtctgggaaa agatccggaa gtccgttaca tcccaaacgg gggcgcagtg gcaaacctgc
      121 aggtggccac gtcagaaagc tggcgcgaca aacagacggg ggagatacgg gagcagacag
      181 aatggcatcg cgtggtgctg ttcggcaagc tcgcggaagt ggctggtgag tatctgcgca
      241 agggcgcgca ggtctacatc gaaggtcagc ttcgcacccg tagctgggag gataacggta
      301 tcacccgtta cgtcaccgaa attcttgtta agaccacggg caccatgcag atgctgggac
      361 gtgccgcagg tactcagact cagccggaag aggcgcaaca gttcagcggt cagcctcagc
      421 cggaatcaca gccggaaccg aaaaaaggag gcgcgaaaac gaaaggccgt ggacgtaagg
      481 ccgcgcagcc ggagcctcag cagccgtctg aacccgcgta tgacttcgat gacgacattc
      541 cgttctaacg aaagacagtg acaaccgccc cgtccagtgc ggggcatcac cggagagatg
      601 aggatgagcg aatatttcag aatacttcag gggctgcctg acggctcctt tacccgcgaa
      661 caggctgaag ccgttgccgc acagtaccgg aatgtcttta tcgaggatga tcagggaacg
      721 cattttcgcc tggttgtccg caataacagt ggcctggtct ggcgcacctg gaattttgag
      781 gacggtgccg ggtactggat gaaccatgtc atccggaatt tcggcattct taagtaagag
      841 aggtgccgga cgcgcaaacg tccggcagga cataagcaat tataagggga tgattatgcc
      901 tgtaacgaag tgtgaaccag aaaccacccg caaagcaagc cgtaaatatg caaaaacgca
      961 ggaaacagtc ctgtctgccc tgctggcgca gacggaggaa gtgagtgtgc cgctggcctc
     1021 actgattaaa tcaccgctga atgtgcgcac ggtgccgtat tctgcggagt ccgtcagcga
     1081 actggctgag tccattaagg gcgttggcct gttgcagaat ctggtcgtgc atactctgcc
     1141 tggtgaccgt tacggtgtcg ccgcaggtgg tcgccgactg gcagcactca acatgctggc
     1201 agagcgtgac atcattccgg ctgactggcc tgtccgcgta aaaattattc cgcaggagct
     1261 ggcgactgcc gcatcaatga ccgagaacgg tcatcgtcgg gatatgcacc ctgccgaaca
     1321 gattgccgga ttccgcgcaa tggcgcagga aggcaaaaca cctgcacaca tcggtgattt
     1381 gctgggctat tcaccccgcc acgttcagcg aatgctgaaa ctggctgacc ttgcgcctgt
     1441 catcctcgat gcgctggcag aagaccgcat caccaccgaa cactgtcagg cgctggcgct
     1501 ggagaacgac accgcgcgtc aggtgcaggt gtttgaagcc gcctgtcagt cgggatgggg
     1561 cggtaaaccg gaagtacaga ccattcgtcg tctggtgacc gaaagtgaag tggcggtggc
     1621 tgggaacagt aaattccgct tcgtgggggc tgatgccttc tcgccagacg aactgcgcac
     1681 cgatttgttc agcgatgacg gggacggtta tgtcgaccgc gtggcgctcg atgccgccct
     1741 gctggaaaaa ctccaggctg tcgctgaaca ccttcgggaa gccgaaggct gggaatggtg
     1801 cgccgggcgc atggagcctg tcggtgagtg ccgtgaggat gccggaacat accgctgtct
     1861 gccggagccg gaagcggtgc tgacggaggc ggaagacgaa cgcctgaacg aactgatgac
     1921 gcgttacgac gcgctggaaa accagtgtga ggaatccgac ctgctggaag cagaaatgaa
     1981 gctgatgcgc tgcatggcga aggtcagagc gtggacgccg gagatacgtg ccggaagcgg
     2041 tgtggtggtg tcctggcgtt atggcaacgt atgtgtccag cgtggtgtgc agttgcgcag
     2101 tgaagatgac gcgactgacg acgctgaccg cacggaacag gtgcaggaga aagcgtcagt
     2161 ggaggaaatc agcctgccgt tgctgacgaa aatgtcctca gagcgcacgc tggcagtcca
     2221 ggcggcactc atgcagcagc cggacaaatc tctggcactg ctggcatgga cgctctgcct
     2281 gaatgtgttt ggcagcgggg cgtacagtaa accagcacaa atcagcctgg aatgtgaaca
     2341 ttattcgctg accagcgatg cgccatcggg gaaggaaggt gccgcattca tggcgctgat
     2401 ggcagaaaaa tcccgtcttg cagccctgct gccggaggga tggtcacggg acatgacgac
     2461 attcctgtcc ctcagtcagg aggtgctgtt atccctgctc agtttctgca ccgcatgcag
     2521 ccttaacggt gtccagaccc gtgagtgtgg tcacacgtca cgcagtccgc ttgactcgct
     2581 ggaaagcgct atcggattcc acatgcgcga ctggtggcag ccgacaaaag caaacttctt
     2641 cggacacctg aaaaagccgc agattatcgc agccctgaat gaggccggac tgtccggtgc
     2701 cgcacgggac gcggagaaga tgaagaaagg tgatgcggct gaacatgcag agcaccatat
     2761 gaaagacaac cgctgggttc caggctggat gtgtgcacca catccacaga cagatgccac
     2821 tgaacgcacc gataacctgg ctgatgccgc ctgatgaaca accacaccgc cccgccggag
     2881 acggggcggc agcaagggag ataccgtgat gaaaactgaa ctgaccctga atgccctgca
     2941 gtccatgaac gcacaggaat atgaagatat ccgtgctgcg ggaagcgata tgcgccgtaa
     3001 tctcactcac gaggtgatgc gtgaagtgga cgcaccggct aactggatga tgaatggcga
     3061 gtatggcagt gagttcgggg gctttttccc cgtccaggtt cgtttcacgc cagcccacga
     3121 acgtttccac ctggcattat gttcgccggg agacgtctct caggtctggg tgctggttct
     3181 ggtgaatgcc ggtggtgagc cgtttgcggt ggttcaggtg cagcggcggt ttgcaccgga
     3241 agctgtcagc cattcactgg cactggcggc gtcactggat gcgcaggggt acagcgttaa
     3301 cgacatcatt catatcctga tggcggaagg aggtcaggca tgagcgcacg ttcacaggca
     3361 ctggttcctc tcagcacaga gcaacaggcc gcatggcggg cggtggcgga gaccgaaaaa
     3421 cgccgccatc agggtaacac gctggctgaa tatccgtatg cgggtgcgtt tttccgctgt
     3481 ctgaacggaa gccgcaggat atcgctgtct gacctgcgat ttttcatgcc gtccctgacc
     3541 gcagaagaac tgcacggcaa ccgtcttcag tggctgtatg ccatcgatgt gctgattgaa
     3601 acacagggcg aggtctgcct ccttcccctt cccggtgatg cagcagagcg cttgttcccg
     3661 tcggtgcggt ttcgtgtcag ggaacgcagt cgccataaat ccgcgctggt catgcagaag
     3721 tacagccgcc agcaggcgag ggaggcagag cagaaagccc gtgcgtacca ggcgctggtc
     3781 gctcaggctg aaatcgaact ggcattccat tcgccggaaa cagtcggaag ctggcatgca
     3841 cgctggagtg accgggtggc agagcatgac ctggaaaccc tgttctggca gtggggcgag
     3901 cgttttccgt cactggccgg aatggaacgc tggcagtggc aggatatgcc gttctggcag
     3961 gttattgcgg aagccagtct tgcagcaaga gaggccggtc acgctgtccg tgagatggag
     4021 cggtggatgg tgccgaacaa actccgggag gcagcgtgat gcggcaacaa tcacacggat
     4081 ttcccgtgaa cggtctgaat gagcggatta ttttcaggga aagtgagtgt ggtcagcgtg
     4141 caggtatatg ggctatgatg tgcccggcgc ttgaggcttt ctgcctcatg acgtgaaggt
     4201 ggtttgttgc cgtgttgtgt ggcagaaaga agatagcccc gtagtaagtt aattttcatt
     4261 aaccaccacg aggcatccct atgtctagtc cacatcagga tagcctctta ccgcgctttg
     4321 cgcaaggaga agaaggccat gaaactacca cgaagttccc ttgtctggtg tgtgttgatc
     4381 gtgtgtctca cactgttgat attcacttat ctgacacgaa aatcgctgtg cgagattcgt
     4441 tacagagacg gacacaggga ggtggcggct ttcatggctt acgaatccgg taagtagcaa
     4501 cctggaggcg ggcgcaggcc cgccttttca ggactgatgc tggtctgact actgaagcgc
     4561 ctttataaag gggctgctgg ttcgccggta gcccctttct ccttgctgat gttgtacggg
     4621 catgaacaac ctgacttcag gaaggtcgtt tcccttcagg aacggggatg aacgcgcgcc
     4681 tgcggcgccc gttcttttcc cccgccttct ctggttatgg cctgtcagaa atcacatcac
     4741 cttccctgtg attattctct ttttcttcct gctctgattc tgactactgc agtgttgtcc
     4801 tgtctgccgg gcgcaccttc ggctcccggc tgcctggcgg catccggtcg tcaggcccga
     4861 ctccgacagg cag
//
LOCUS       DAHHTG010000215         4752 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.219, whole genome shotgun sequence.
ACCESSION   DAHHTG010000215 DAHHTG010000000
VERSION     DAHHTG010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4752)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4752)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4752
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.219"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            137..415
                     /locus_tag="NBI74_004392"
     CDS             137..415
                     /locus_tag="NBI74_004392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016240842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1934618.1"
                     /translation="MKDLTLKFHDKLQFKAFLSSLGWAEDEDLQNKLLVDEIGFTYTE
                     TGVTEEGEPVCIRNDGYFVNIRILDDLFDVSVFSDYVVELETPLREWS"
     gene            428..721
                     /locus_tag="NBI74_004393"
     CDS             428..721
                     /locus_tag="NBI74_004393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000355605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1934619.1"
                     /translation="MDISPLLHALCAVAAQILVGLFTGNWAYGAIAGCTFFIAREHTQ
                     AEYRWIEMFGHGKRMNMPWWGGFDPRAWDVASLMDFAVPVVACLLVWLLVNRG"
     gene            complement(813..1670)
                     /gene="sitD"
                     /locus_tag="NBI74_004394"
     CDS             complement(813..1670)
                     /gene="sitD"
                     /locus_tag="NBI74_004394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter permease subunit
                     SitD"
                     /protein_id="HCD1934620.1"
                     /translation="MMALLLEPLQFTFMSHALLISLVVSIPCALLSVFLVLKGWALMG
                     DAMSHAVFPGIVLAWILGLPLATGAFVAGVFCAVATGYLKDNSRIKQDTVMGIVFSGM
                     FAAGLILYIAVKPDVHLDHILFGDMLGITIGDIIQTMIIAGLVTLVISVKWRDFLLFS
                     FDYQQAQVSGLHTRWLHYGLLCMVSLTIVATLKAVGIILSISLLIAPGAIAVLLTQRF
                     HIALLLATGISVIVSMTGVWLSFFIDSAPAPTIVVLFAVLFIMTFAVTSINARKKGNS
                     HTQDLLSPN"
     gene            complement(1667..2524)
                     /gene="sitC"
                     /locus_tag="NBI74_004395"
     CDS             complement(1667..2524)
                     /gene="sitC"
                     /locus_tag="NBI74_004395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter permease subunit
                     SitC"
                     /protein_id="HCD1934621.1"
                     /translation="MNVLLEPFSYEYMLNAMWVSAMVGGLCAFLSCYLMLKGWSLIGD
                     ALSHSIVPGVAGAYMLGLPFSLGAFFSGGLAAGSMLFLNQRTRLKEDAIIGLIFSSFF
                     GLGLFMVSLNPTSVNIQTIVLGNILAIDPADILQLTIIGILSIIVLFFKWKDLMVTFF
                     DENHARAIGLHPGRLKLLFFTLLSVSTVAALQTVGAFLVICLVVTPGATAWLLTDRFP
                     RLLMIAVTIGSVTSFLGAWVSYFLDGATGGIIVVAQTLLFLLAFVFAPTHGLLANRRR
                     AHKALEDRS"
     gene            complement(2521..3348)
                     /gene="sitB"
                     /locus_tag="NBI74_004396"
     CDS             complement(2521..3348)
                     /gene="sitB"
                     /locus_tag="NBI74_004396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter ATP-binding
                     protein SitB"
                     /protein_id="HCD1934622.1"
                     /translation="MMQSAGIVVNDVAVTWHNGHTALRDASFTVPSGSITALVGVNGS
                     GKSTLFKAIMGFVRLTSGKISVLGIPTRQALQKNLVAYVPQSEEVDWSFPVLVEDVVM
                     MGRYGHMGMLRIAKKRDRQIVTDALERVDMVNFRHRQIGELSGGQKKRVFLARAIAQQ
                     GDVILLDEPFTGVDVKTEAKIISLLRELRAEGKTMLVSTHNLGSVTTFCDYTVMVKGT
                     VLASGPTDTTFTAENLELAFSGVLRHVTLNGSEESIITDDERPFVAHRPSAVQREER"
     gene            complement(3348..4262)
                     /gene="sitA"
                     /locus_tag="NBI74_004397"
     CDS             complement(3348..4262)
                     /gene="sitA"
                     /locus_tag="NBI74_004397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000949004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter substrate-binding
                     protein SitA"
                     /protein_id="HCD1934623.1"
                     /translation="MRSIKKVTMLLGGLALTCSIAFQASATEKFKVITTFTIIADMAK
                     NVAGDAAEVSSITKPGAEIHEYQPTPGDIKRAQGAQLILANGMNLELWFQRFYQHLNG
                     VPEVIVSSGVTPVGITEGPYEGKPNPHAWMSPDNALIYVDNIRDALIKYDPANAQTYQ
                     RNADTYKAKITQTLAPLRKQITELPENQRWMVTSEGAFSYLARDLGLKELYLWPINAD
                     QQGTPQQVRKVVDIVKKNHIPAVFSESTISDKPARQVARETGAHYGGVLYVDSLSTEN
                     GPVPTYIDLLKVTTSTLVQGIKAGKREK"
BASE COUNT         1322 a         1132 c         1150 g         1148 t
ORIGIN      
        1 ataatggcgt gaccgctgat aaaaatatcc gatttggagg gcagacgaat actggagaac
       61 gacatctgtt tggccatatt cgcaatttcc gcatatggca taaagaatta aatgacaggc
      121 aattaaaaga ggtcgtatga aagatttaac tttgaagttt catgacaaac tgcagtttaa
      181 ggccttcctg tcatctcttg gctgggcgga agatgaagac ctccagaata aactgttagt
      241 tgatgaaatt ggtttcacct acacagaaac aggggtaaca gaagagggag aacctgtctg
      301 tatccggaat gatggttatt ttgtcaacat tcgcattctt gatgacttgt ttgatgtttc
      361 tgtattctct gattatgtcg tggagctgga aacaccgctt cgggaatgga gctgaaagga
      421 ggaaataatg gatataagcc ccttacttca tgcactttgt gctgtggctg cgcagatact
      481 ggttggtctt tttaccggaa actgggctta cggagcgata gccggttgta cgttcttcat
      541 tgcgcgtgaa catacccagg cagaatatcg ctggattgaa atgttcgggc atggcaagcg
      601 aatgaatatg ccgtggtggg gcggttttga tccgcgtgct tgggatgtgg caagcctgat
      661 ggattttgct gtgccggtgg tggcgtgtct gctggtctgg ctgttggtta atcgtgggtg
      721 aaaaaaggtg agctgtatat gcaacggagg aagaaacccc gttgctggaa gccaggaaaa
      781 agtatcgggt gttgctgtcc tcggatgttg tgctaattgg gtgataacag atcctgtgta
      841 tgggagtttc cctttttgcg tgcgttgatg ctggtaacgg caaacgtcat gatgaacaaa
      901 accgcgaata agacgacaat cgtcggagct ggtgcgctgt cgataaaaaa ggacaaccag
      961 acaccagtca ttgacactat taccgatatg ccagtcgcca gtaggagcgc aatatgaaag
     1021 cgttgtgtca ggagtaccgc aatggcgcca ggcgcaatga gcagagaaat ggagagaatg
     1081 atacctaccg ctttcagcgt cgccacaatg gtcagagaga ccatgcacag cagcccataa
     1141 tgcagccatc tcgtatgcaa accgcttacc tgcgcctgtt gataatcaaa gctgaacagc
     1201 aaaaaatccc gccattttac gctaataaca agtgtaacca gcccggcaat aatcatcgtc
     1261 tggattatat cgccgatggt tatcccgagc atgtcgccga aaagaatatg gtcaagatgt
     1321 acgtctggtt tgactgcaat atacaagatc agacctgcgg caaacatgcc agaaaaaaca
     1381 atccccatca ctgtatcttg cttgattcgg ctgttgtctt tcaggtatcc cgtcgccacc
     1441 gcgcagaata ctccggcgac gaatgcccct gtagccaaag gtaaccccag aatccaggca
     1501 agcacgatgc caggaaaaac cgcatgactc atcgcgtcac ccatcagtgc ccaacctttc
     1561 aggaccagaa agaccgacag cagcgcacag ggtatggaaa ctaccagtga aatcagcagt
     1621 gcgtgactca taaaggtgaa ctgcagtggc tccagtaata atgccatcat gagcgatcct
     1681 ccagcgcctt atgcgcacgc cgacgatttg ccaaaagccc atgggtgggc gcaaagacaa
     1741 atgctaaaag aaacagcagt gtctgcgcca ccacgatgat tccacctgtc gcgccgtcca
     1801 gaaagtaact gacccacgcg ccgaggaaac tggttacact gccaatagtt acagcaatca
     1861 tgagcaggcg gggaaaacga tcggttaaca accatgctgt tgcaccggga gtcaccacca
     1921 gacagatcac cagaaaggca ccaaccgttt gcagcgccgc aacggtcgaa accgacagca
     1981 gggtgaagaa gaggagtttt aatctgcccg gatgcaatcc aatagcacgg gcgtgattct
     2041 cgtcgaaaaa ggtcaccata agatctttcc atttgaaaaa aagcacgatg atcgagagaa
     2101 tgccaatgat agtgagttgc aggatatccg ccggatcgat tgccagaata ttgcccagga
     2161 cgatggtctg aatgttcaca gatgtcgggt tcaatgacac cataaacagc cccaggccaa
     2221 aaaaggacga gaagatcaga ccgatgatgg catcttcttt taagcgggtg cgttggttaa
     2281 gaaacagcat gctgcccgca gccagtccgc cggaaaagaa ggctcccaga gaaaacggaa
     2341 gccccagcat atatgccccc gcaacacccg ggacaataga gtgcgacaac gcatcgccaa
     2401 tcagcgacca gccttttagc atcagatagc atgacaggaa agcgcagaga ccgccgacca
     2461 tcgccgagac ccacatcgca ttgagcatat attcatagct gaagggttcc agtagcacgt
     2521 tcatctttct tccctctgca ccgctgacgg tcgatgagcc acaaaagggc gttcatcgtc
     2581 agtaataatg ctttcttccg agccgttaag tgtgacatgg cgcagtacgc cgctaaaagc
     2641 cagctctagg ttctcggctg taaaagtggt gtctgtcggc ccgcttgcca ggacggtgcc
     2701 tttgaccatg accgtataat cgcaaaatgt cgtgacggat ccgagattgt gtgttgagac
     2761 aagcatcgtt ttgccttccg cgcgtaactc cctcaacagg ctgatgattt tggcttcggt
     2821 tttcacatca acgccagtaa aaggttcatc cagcaggatc acatcaccct gctgtgcaat
     2881 cgctctcgcc agaaagacgc gtttcttttg tccaccggaa agctcgccga tttgtcgatg
     2941 gcgaaaattc accatatcga cacgttccag cgcatcggtg acaatttgac gatccctctt
     3001 tttggcgata cgcaacatac ccatatgccc atagcggccc atcatcacca catcttccac
     3061 cagtacagga aatgaccagt caacctcttc tgactgaggg acgtaggcaa ccaggttttt
     3121 ctgtaacgcc tgtcgcgtgg gaatacccag aacagatatc tttccgctgg tcagacgcac
     3181 aaatcccata atcgctttaa acaacgtcga ttttccggaa ccgtttaccc caaccagagc
     3241 ggtaatagat ccacttggta ctgtgaagga tgcatcgcgc agtgctgtgt gcccgttatg
     3301 ccaggtgaca gcaacatcat taacgacaat gcctgcagat tgcatcatta tttctccctt
     3361 ttcccggctt taattccctg taccagcgta ctggtagtaa ccttaagaag gtcgatatac
     3421 gtcggtaccg ggccgttttc tgtgctcagg gaatcgacat agagcacacc gccgtagtgt
     3481 gcgccggttt cacgcgcaac ctgacgcgct ggtttatcgg aaatcgtact ctcgctaaag
     3541 actgccggga tatgattttt cttaactata tcaacaacct tacgtacctg ctgcggtgtt
     3601 ccttgttgat cggcattaat cggccacaga taaagctctt ttagccccaa atcgcgtgcg
     3661 agataagaaa aagccccttc actggtgacc atccatcgct gattctcagg gagttccgta
     3721 atctgcttac gcaggggggc aagggtttgg gtaatcttgg ctttataagt atcggcattg
     3781 cgttggtagg tttgtgcatt tgccggatcg tattttatca acgcatcacg aatattatcg
     3841 acgtaaatca gagcattatc tggcgacatc catgcatggg ggttaggttt gccctcatag
     3901 ggtccttcgg tgatccctac aggcgtcaca cccgaagaga caattacttc tggaacccca
     3961 ttgagatgct ggtaaaagcg ttggaaccac aattccagat tcataccatt ggcgagaatc
     4021 agttgtgccc cctgcgcacg tttaatatcg ccaggggtag gctgatactc atgaatttct
     4081 gcaccaggct tggttatgga tgagacttct gcagcatctc ccgccacgtt tttggccata
     4141 tccgcgatga tggtaaatgt tgtaatgacc ttaaattttt cagttgcact tgcctgaaat
     4201 gcgatcgagc aggtgagtgc gaggcccccc aagagcatgg ttactttttt tattgagcgc
     4261 ataatttagt acctatagtg agcaaaatta gaacaaacat agcaacggct atgttttata
     4321 gcataaccta tacagaatga aaatgattat cattaacatc tttgaggccc aaaacaggga
     4381 ttagtttgtt tcctgctgga tgaaaggact cttgggatag cacatgccgc acgaagagca
     4441 gcattacatg atgttttgtc gaatgagcta cgaatcctga tagaaatacg aaacaagcta
     4501 gcacatggac aatggatcta tcccttcaat aatgaaggaa atactgtgga ccctgataaa
     4561 tataaattaa tcaataaaga aaacttgcag tcactccaat tcaaatattc attagcacag
     4621 catttagttg acgctgtaca tgatttggtt gtaagtccta cgacattcga acgagatttc
     4681 gaaagccatt tttaaaaatt atatcaagta cgaacaaact tagtgacaaa agactacgaa
     4741 aaatataaaa ca
//
LOCUS       DAHHTG010000216         4717 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.220, whole genome shotgun sequence.
ACCESSION   DAHHTG010000216 DAHHTG010000000
VERSION     DAHHTG010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4717)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4717)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4717
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.220"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            314..520
                     /locus_tag="NBI74_004398"
     CDS             314..520
                     /locus_tag="NBI74_004398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001564245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family transcriptional regulator"
                     /protein_id="HCD1934624.1"
                     /translation="MTTPVSLMDDQMVDMAFITQLTGLTDKWFYKLIKDGAFPAPIKL
                     GRSSRWLKSEVEAWLQARIAQSRP"
     gene            615..1217
                     /locus_tag="NBI74_004399"
     CDS             615..1217
                     /locus_tag="NBI74_004399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001546339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1934625.1"
                     /translation="MKQQYQTRYEWLHESYQKWLTGFTRHAVSWGVCHPNIYYFHNLT
                     PGWVSFNGEKPEIAIVPQSLHRLIYGPDKRATPPLDDDLIVNLCTSEHLLVHHPMLEG
                     ILLSECERLKHHSLANKLISLFRQFGGTELRLKLVWLCWLDLMTGNSLEDWTENLKRK
                     SEKELEEWIIDRQKQSAALTDLMDQYVLLAYRTTVDDNRN"
     gene            1854..2233
                     /locus_tag="NBI74_004400"
                     /pseudo
     CDS             1854..2233
                     /locus_tag="NBI74_004400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001750026.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            3805..4677
                     /locus_tag="NBI74_004401"
     CDS             3805..4677
                     /locus_tag="NBI74_004401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001767894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1934626.1"
                     /translation="MVKVVEDERSRIRYLERRLNENGFYLPSSLADKDYFSYQKRILN
                     TLISQGADTLKINNFLAETDQRYFDSLPSEDDLNWYRNDARASLWLTCELYEMIKING
                     YENTLTCLSPESLPSHHSVRVDAIRRCIDNWPFILYTPSNYLNQKSIEWTTLLEKDDI
                     FREVKARNFDICSWLKKYIQEKTNISLNYVCGESSEEIMAWCYASYFTWKKNNQNSPD
                     SVELFTRKFKSAWATQKNRIKNRVDKKLRPLNVNISQEAYDKLRKLSINEGISNDRVI
                     ESALDMIYRSKIKK"
BASE COUNT         1402 a         1097 c         1053 g         1165 t
ORIGIN      
        1 cctgcccaca aggcacgggc tgccccaccg taaccgcccc gtaaataact ctgcaattat
       61 cagaacgcca gttttcccct cttcctcacg aaaatcatcc cttctttatc gcgcgcgcgt
      121 gcggaagcat cgtttcgtaa caaccacccg gcataccggt acggctcagc caccgcaacc
      181 tccccgttta tctcctggcg gtcacattcc cgattatttt ttacgtttct ccccggttgt
      241 tatgccggtg aaggtggtgc gttgttttca tgaccacacc ggctggttta acaacatccg
      301 gaggaacatt ctcatgacca ccccagtttc gctgatggat gaccagatgg tcgatatggc
      361 gtttatcact cagctgaccg gcctgaccga taagtggttt tacaagctca tcaaggatgg
      421 ggcctttccg gctcccatca aactcggccg cagctcccgc tggctgaaaa gtgaagtgga
      481 agcctggcta caggcgcgta ttgcacagtc ccgtccgtaa tttctgcccc ttatccgttc
      541 acccgcagca gacgtaatcc ccggcctgcc gacggcattc tgctgcctgt tatatccccg
      601 tgaggaatat gaaaatgaaa caacagtatc agacccgcta cgaatggctc cacgaaagct
      661 accagaaatg gctgaccggc ttcacccggc acgccgtatc ctggggcgtg tgtcatccga
      721 atatctacta cttccataat ctgacgcccg gttgggtgtc attcaatggt gaaaaaccgg
      781 agattgccat cgtcccccag agcctgcacc ggctgattta tggccctgac aaacgggcca
      841 cgccgcccct ggatgatgat ctgatagtga atttatgcac cagtgaacat ctgctggttc
      901 atcatccgat gctggaaggc attctgctgt ctgaatgtga acgtctgaaa caccattctc
      961 tggcaaataa actgatcagt ctcttccgtc agtttggtgg cacggagtta cgccttaaac
     1021 tggtctggct ttgctggctt gatttaatga ccggaaacag ccttgaagac tggacagaga
     1081 acctgaaacg gaaatcagaa aaagagctgg aggaatggat catcgaccgt cagaagcaga
     1141 gcgcagcact gacggatctg atggatcagt acgtgctcct ggcataccgc acaacggttg
     1201 acgataaccg taactgatgc cggttgcttc cggaaggtgc ggcaaaatac gggaatgatg
     1261 cttccggccc cacatactca cctgcagagc gtgttcgtcg tcatcatact gagccatgac
     1321 ggatggctta agggcatcct gtcattgctg aactggcatg aacaccacca gtgaatgaaa
     1381 gccatggaca gggatgttct ttacagccag atacacgcca cgttctctac ggactgaata
     1441 tgcaggcaga ggttatttac cagaaatggc atgctgcggt acatgcctca tcctggtgac
     1501 aataagtaca tggcgacagc gctccttgca ctgcggaaga aactcacacg gcaggatgtt
     1561 atggctgaag tccccggaga tcattgtttc ttcgtcagat attcaggaac agttgtagta
     1621 acgaatttta ccggtctctg cacatggccc ggctccgtct gtgtggcaca ggttacgggt
     1681 attgccggtt gtaggaacgg acggtgagtt gagtgatctg tggaatcagg gagcattctg
     1741 ggcattatgg tgctgaaatg tgaccatcga cacaataccc ctgcttcgtg gtacacaata
     1801 accaggaaac ataccctgaa ccgacagcca taaggacccg tcacaaaatg gtgctaatga
     1861 aagcgaaaca ggaaaaccgg cttgagcgac agctccaata actctgttac gcacgcgtac
     1921 tgatccttga tgaaacaggc tatctgccaa tgccccggaa agaagccagg ctgttcttcc
     1981 ggttgctgaa gcggcaatat gaaaaagcga gaatcgttct gacgtcaaat aaaggattcg
     2041 ccaactgggg ggaaatgctc ggcgataatg tactggcaac agtgatccct gagcatctgc
     2101 tgcatcactc aaccacgctg aatattaagg ggggaaagct accacctgaa ggaaaaacgt
     2161 aaagcaggtg tactggcaag ggccagcgcc aataagtgat gatgaatcgg tggaaaccgg
     2221 gcatcgccat tgataaatag cggcaatcaa ataaaacggt gaaaaaaggt tatatacagc
     2281 ggcgttgaca atacaggagg taatactcac caataaataa gcgtcaatac ggtgctccgt
     2341 tgacgcttac cattgctcat tcagttgagc aataaattgt cccggataat ccagtccttc
     2401 acattgtctg ctaccagatg aaacttgcgc ccctatccgg ggctgtcact ccatgcagcc
     2461 agactttatg tttcccttca ccagaatacc cccagagtga taatgtcgat aaccagatgt
     2521 ggaatttcgc agagagctgg cttctgatgc tgacgctaac gtgcttttcc cacccgctaa
     2581 ccagaaagta atctgctcag catatcagta accccatcag cttaaaatca aatcaataaa
     2641 gccctgtaaa acctgaagta acagtccggg aactcgtatc atgctaacat atcagtgctg
     2701 tgaagcagtc gacaacgagc ttcagacggt acacacgtca attcatgcag aaatagcagc
     2761 cacatcaggt ctggctgtca gcggaccacg cagcctgagt acattgttgt aaattaaacg
     2821 actgatgggt tttaaatttt ttctttgcca ccaccgtgaa ctgataccgg aaaaacagta
     2881 gtctcaggaa gcgtgatctg aaagtcagct ttattaaaaa agctattaga ccgggtaaat
     2941 aatgtcaacg gatgaaatgt tatgataatt ctggatgcca tatctatcag taaatcagta
     3001 acgccagagg gtatggtaat atctaagaaa tactttacgg taatcaggaa tatttacttg
     3061 ccatcatggc ttatgaaatt catggttcat gagtcactgt aactcccgta tcgtccgtca
     3121 attatagttt acgttaacga tatatctgtg tctgcaggca ctacaatgaa ttcccgaata
     3181 aatttccgac cagtcatttt ctctgacaag gaagcccata tgatttccct gtacccgtga
     3241 cagactggtg aacaatacca tatctgtaag atatgattaa tgaggaaagt gatgaccgtt
     3301 agtcatttta tctccagtaa cgtaaaacca gacttatcgt aaataaaaat atgaagtcct
     3361 ctgttaaagt catggagaga cgcatcaaaa tacaggatac aatgattctc acaatgaaac
     3421 tcatcgtctc tggtgaaatt catatcaaca tttaccttaa taacctgacg cgggattaac
     3481 tgtgttgttt aaggaaaagg ggagagttgc ccgaaggcga aaacgaaagt aaattaataa
     3541 taacaggatg agaggagaaa gcgctcttcc cggaaaataa aaagagcggt tcactcctgt
     3601 gaatgcactt caaccgtcct ggcaaagaat gtacttttat aaaaccccct cccagtcagc
     3661 agatacaaaa agataacatg aggtatctcc cgccccttaa tttatacacg tgaaatgaac
     3721 tgaaatttat gagaaaatca tgctaaagat gctttgattt tacttaaaat cacgtgtgat
     3781 tatcctgact gaaggagaga tactatggtt aaggttgtgg aagatgaaag aagcagaata
     3841 cgatatcttg aaaggcgctt aaatgaaaat gggttttatt taccatcaag cctggccgat
     3901 aaagattatt tctcgtatca gaaacggatt ttaaacactc ttatttcaca gggtgctgac
     3961 acactgaaaa taaataattt tctggctgaa acagaccagc gttacttcga ttccttacca
     4021 tctgaggatg atcttaactg gtatcgtaat gatgcccgcg ccagtttatg gttgacttgt
     4081 gaattatatg aaatgattaa gataaacggt tatgaaaata cccttacctg tttatctccg
     4141 gagtcactgc cttctcacca cagcgtgagg gtggatgcca tacgcaggtg tattgacaac
     4201 tggcctttca ttttatacac accatcaaat tacctgaacc agaaaagtat tgaatggacc
     4261 acactactcg aaaaagatga tattttcaga gaggttaagg ccagaaattt tgatatctgc
     4321 tcatggctca aaaaatatat tcaggaaaag acgaacattt ccctgaacta tgtctgtgga
     4381 gaatcatctg aagagattat ggcctggtgt tatgccagtt acttcacatg gaaaaaaaac
     4441 aaccagaatt caccagactc agtagaactg tttacccgga agtttaaaag cgcctgggct
     4501 acccaaaaaa acagaataaa aaatagggtt gataaaaagc tgcgccccct gaacgtaaat
     4561 atttcacagg aggcatatga caaactgaga aaattatcca taaacgaggg tatctcaaat
     4621 gaccgggtta ttgaaagtgc tctggacatg atttaccgta gcaaaattaa aaaataaaac
     4681 cattcaaaat aatttcagac agatattatc tccgtga
//
LOCUS       DAHHTG010000217         4700 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.221, whole genome shotgun sequence.
ACCESSION   DAHHTG010000217 DAHHTG010000000
VERSION     DAHHTG010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4700)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4700)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4700
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.221"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            <1..655
                     /gene="hprR"
                     /locus_tag="NBI74_004402"
     CDS             <1..655
                     /gene="hprR"
                     /locus_tag="NBI74_004402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator transcription factor HprR"
                     /protein_id="HCD1934627.1"
                     /translation="EDNQRTQEWVTQGFSEAGYVIDAVSDGRDGLYLALKDDYALIIL
                     DIMLPGMDGWQILQTLRTAKQTPVICLTARDSVDDRVRGLDSGANDYLVKPFSFSELL
                     ARVRAQLRQHHALNSTLEISGLKMDSVSQSVSRDNISITLTRKEFQLLWLLASRAGEI
                     IPRTVIASEIWGINFDSDTNTVDVAIRRLRAKVDDPFPEKLIATIRGMGYSFVAVKK"
     gene            655..2013
                     /gene="hprS"
                     /locus_tag="NBI74_004403"
     CDS             655..2013
                     /gene="hprS"
                     /locus_tag="NBI74_004403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     HprS"
                     /protein_id="HCD1934628.1"
                     /translation="MKRLSITVRLTLLFILLLSVAGAGIVWTLYNGLASELKWRDDTT
                     LINRTAQIKQLLIDGVNPDTLPVYFNRMMDVSQDILIIHGDGINKIVNRTNVSDDMLN
                     NIPASETISAAGIYRSIINDTEIDAFRINIDEVSPSLTVTVAKLASARHNMLEQYKIN
                     SIIICIVAIILCSVLSPLLIRTGLREIKKLSGVTEALNYNDSRVPVEVNALPRELKPL
                     GQALNKMHHALVKDFERLSQFADDLAHELRTPINALLGQNQVTLSQTRSIAEYQKTIA
                     GNIEELENISRLTENILFLARADKNNVLVKLDSLSLNKEVENLLDYLEYLSDEKEICF
                     KVKCNQQIFADKILLQRMLSNLIVNAIRYSPEKSRIHITSFLDANGSLNIDIASPGTK
                     INEPEKLFRRFWRGDNSRHSVGQGLGLSLVKAIAELHGGSATYHYLSKHNVFRITLPQ
                     RN"
     gene            complement(2121..2972)
                     /gene="hchA"
                     /locus_tag="NBI74_004404"
     CDS             complement(2121..2972)
                     /gene="hchA"
                     /locus_tag="NBI74_004404"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein deglycase HchA"
                     /protein_id="HCD1934629.1"
                     /translation="MTVQTSKNPQVDIAEDNAFFPSEYSLSQYTSPVSDLDGVDYPKP
                     YRGKHKILVIAADERYLPTDNGKLFSTGNHPIETLLPLYHLHAAGFEFEVATISGLMT
                     KFEYWAMPHKDEKVMPFFEQHKSLFRNPKKLAEVVASLNADSEYAAIFVPGGHGALIG
                     LPESQDVAAALQWAIKNDRFVISLCHGPAAFLALRHSDNPLNGYSICAFPDAADKQTP
                     DIGYMPGHLTWYFGEELKKMGMNIINDDITGRVHKDRKLLTGDSPFAANALGKLAAQE
                     MLAAYAS"
     gene            complement(3567..4625)
                     /locus_tag="NBI74_004405"
     CDS             complement(3567..4625)
                     /locus_tag="NBI74_004405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001080680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HCD1934630.1"
                     /translation="MKRKVLAMLVPALLVAGAANAAEIYNKNGNKVELYGKMVGERIL
                     TDRENGEKGDNSQDTSYARVGVKGETQINPELTGYGQFELDLEASNRHNPDQTRLAYA
                     GLSYKDFGSFDYGRNVGVAYDAEAFTDMFVEWGGDSWAGTDLFMTNRTNGVATYRNTD
                     FFGMVEGLNFALQYQGKNEGTGNYKANGDGHGLSATYTIDGFSFAGAYANSDRTDWQS
                     GDGKGERAEVWALSTKYDANNVYAAVMYGESHNMNSDDGDVVNKTQNFEAVLQYQFDF
                     GLRPSIGYSYSKALDVAGYKDSDRLNYIEIGTWYYFNKNMNVYTAYQINLLDKSDYVL
                     AHGLNTDDQLAVGIVYQF"
BASE COUNT         1319 a          940 c         1090 g         1351 t
ORIGIN      
        1 tgaagataat caaaggaccc aggaatgggt aacgcagggg ttttccgaag cgggttatgt
       61 cattgatgcc gtttctgatg gcagagatgg gctttatctt gcgctgaagg atgattatgc
      121 attgatcatt ctggatatta tgcttccggg tatggatggc tggcagatct tacaaacgtt
      181 aagaacagca aagcaaaccc ctgttatttg ccttactgca agggattctg tcgatgacag
      241 agtcagagga ctggacagtg gggcaaatga ttatctggta aaaccttttt cattttctga
      301 gttgctggca agagttcggg cacaattaag gcaacatcac gctctgaatt caacattaga
      361 aatcagtgga ttaaaaatgg actctgtcag tcaaagtgtg agcagggaca atatcagtat
      421 tacactgacg cgcaaggagt ttcagttact ttggctactg gcctccagag ctggcgagat
      481 tatacccaga acggttattg cgagtgaaat ttggggaatc aactttgata gtgataccaa
      541 tacggtggat gtcgccattc gcaggcttcg cgcaaaagtt gatgatcctt ttcctgaaaa
      601 gctaatcgct acaatccggg ggatgggcta ttcattcgta gcggtaaaaa aataatgaaa
      661 agattatcta taaccgtccg tttaaccttg ctttttatat tgctgctgtc tgttgctggc
      721 gctggaattg tatggactct ctataatggc ctggcaagtg agttgaaatg gcgcgatgat
      781 acaacactca ttaaccggac agcgcagatc aagcagttgt taattgatgg ggtaaatcca
      841 gatacattac ctgtctattt taaccggatg atggatgtta gtcaggatat cttgatcatt
      901 catggtgatg gcatcaataa aattgttaac cggacaaatg tcagtgatga catgttaaat
      961 aacatacctg ctagtgagac aatcagcgca gcaggcattt acagaagcat tattaatgat
     1021 acagagatag atgctttccg aattaatatt gatgaagttt cgccatcatt aacggttact
     1081 gtggctaaat tggcttcagc cagacataac atgcttgaac agtataaaat taatagcatt
     1141 ataatttgca ttgtcgccat tatactgtgc tcagtattaa gtccgttgtt aatcaggacg
     1201 gggttgcgag agatcaaaaa gttgagtggc gtaacggaag cgctgaatta taacgatagc
     1261 cgagtgccgg ttgaggttaa cgcattaccg agagaactaa aacctcttgg gcaggcgttg
     1321 aataaaatgc atcatgcttt agtcaaagat tttgagcgcc ttagtcaatt tgctgacgat
     1381 ctcgctcatg aacttagaac gccaattaat gcattactgg gtcagaatca ggttacgctc
     1441 agtcaaacca gaagtatcgc tgaatatcaa aaaacaattg ccggaaacat tgaagagctg
     1501 gaaaatattt cgcggttaac agagaatata ctgtttcttg ccagggcaga caaaaacaat
     1561 gttttggtga aactggattc gctttctctc aataaggaag ttgaaaatct tttggactat
     1621 cttgaatatc tttcagacga gaaagagatt tgctttaagg tcaagtgcaa tcaacaaatc
     1681 tttgcggata aaattttact gcaacgaatg ttatcgaatc ttattgttaa cgccattcga
     1741 tattcgccag aaaaatcgcg tattcatata accagttttc ttgatgctaa tggttctctt
     1801 aatattgata tcgccagtcc tggaacgaaa attaatgagc ctgaaaaact cttccgtaga
     1861 ttttggcggg gagataattc gcgtcattcc gtaggtcagg gacttggcct ttctttagtc
     1921 aaagcgattg ccgaattaca tggaggaagt gctacgtatc actatctcag taagcataac
     1981 gtgttccgaa ttacgttacc gcaaagaaat taacatcggg cttcagtacg ccataagtta
     2041 accgtgaaaa aacgcagcca cgtcgtgagt attaacgtgg ctgcgattga ttatgcgctt
     2101 acattacaat gtaacaggga ttaactcgcg taagctgcca gcatttcctg cgccgccagt
     2161 ttacccaacg cattcgctgc gaaaggactg tcgccggtga gaagtttacg gtccttatgt
     2221 actcgcccgg tgatgtcgtc attaatgatg ttcatgccca ttttcttcag ctcttcgccg
     2281 aagtaccagg tgagatgacc cggcatatag ccaatatctg gcgtttgttt gtctgcggcg
     2341 tctgggaatg cgcaaatgga ataaccattc agtgggttat cgctgtggcg aagtgccaga
     2401 aaagccgccg gaccgtggca aagggagata acaaaacggt catttttgat tgcccactgt
     2461 aaagcggcag ccacgtcctg gctttctggt aaaccaataa gtgcgccatg cccaccagga
     2521 acaaagattg ctgcatattc gctatcagcg ttgaggctgg cgacaacctc cgcgagtttc
     2581 ttcggattgc ggaataacga tttatgctgt tcaaagaatg gcattacttt ttcatctttg
     2641 tgcggcatag cccagtattc aaacttggtc atcagaccgg aaatggtcgc cacttcgaat
     2701 tcgaaacctg cagcatggag atgatacaac ggcagcaacg tttcaatcgg atggttaccg
     2761 gtcgagaaca gttttccgtt atcggtcggc aaataacgtt cgtccgcggc gatcaccaga
     2821 attttatgtt taccgcgata cggttttgga tagtccacgc catcaagatc agagacggga
     2881 ctcgtatatt ggctaagcga atattctgaa gggaagaatg cattatcttc agcaatatca
     2941 acctgcgggt ttttacttgt ttgaacagtc atagtgtatt ccttattgtt gcttagttag
     3001 ggtagtcact atatttgcga ctgagctggt cgctattttt aaccaggctg gaaaattgag
     3061 tcaatatatc gaaagcggta gtcggcgggt gtaagtatgt aatgatgatg ctgcactgtt
     3121 gttgaatatt attcacgcca aattttcctc attgagcaat gtcgtcgcaa gtggcctcaa
     3181 tgattttaga ccattatcag aggtttgggg gcgatgggga gagatttagt gctgtggaat
     3241 gttgtggtac tgatgaatat ttatatcccg gattggtagg gaaaaagctt ttttcatgca
     3301 ctatttttgc aaatatttct atatgggaag cggttttgtt acagattaaa tttataaaga
     3361 aatgcatttg gggtgttggg ttgaagagta gctggtagat gttattgtgc caggaggggc
     3421 aggggaatgt gccgtatcga tatgctttgg caattgcgtg cgccgcagat ggcgatgaaa
     3481 tgccaataaa aaagcctgag ttcgatgagg gacgaactca ggctggtgtt atgttttaca
     3541 gtaacacgcc tcggcgcgtt ggctgattag aactggtaga cgataccaac tgccagttgg
     3601 tcgtcggtgt tcagtccatg agcgagaaca taatcactct tatcaagcag gttgatctga
     3661 taagcggtat atacgttcat attcttattg aagtaatacc aggtaccgat ttcaatatag
     3721 ttcagacgat cagaatcttt gtaacctgct acgtccagtg ctttagaata ggagtaaccg
     3781 atggatggac gcagaccaaa gtcgaactga tattgcagaa cagcttcgaa gttttgtgtt
     3841 ttgttcacaa catcaccgtc gtcggaattc atgttgtgag attcaccgta cattacagca
     3901 gcatagacat tgttagcgtc atattttgtt gacaacgccc aaacttcggc tctttcgcct
     3961 ttaccatcac cagactgcca atcagtacgg tcagagtttg cataagcacc agcaaatgag
     4021 aaaccgtcaa tagtgtaagt tgcagataaa ccatggccat cgccatttgc tttgtagttg
     4081 cctgtacctt catttttacc ctgatattgc agagcaaaat ttagaccttc aaccatgcca
     4141 aagaaatctg tgttacgata ggttgcaaca ccgttggtac ggttggtcat gaacaggtca
     4201 gtaccagccc atgagtcacc gccccactca acaaacatat cagtaaatgc ttctgcatca
     4261 tacgcgacac cgacattacg accgtagtcg aatgaaccaa aatctttgta actcaaacca
     4321 gcatatgcca aacgagtttg gtcagggttg tgtctgtttg atgcttccag gtctagttca
     4381 aactgaccgt aaccagtcag ttcagggttg atctgagttt cacctttcac gcctacacgg
     4441 gcataagagg tatcttgaga attatcgcct ttttcaccat tttcacggtc ggtcagaatg
     4501 cgctcaccaa ccatcttgcc gtatagctca actttattgc catttttatt ataaatttca
     4561 gccgcatttg ctgcgccagc aactaataac gccgggacca gcattgccag aacttttctt
     4621 ttcattatgt attcccttgt gattataatc ttcatgaata tatcaataag tgctgttatc
     4681 caaaaaaagc acatttggat
//
LOCUS       DAHHTG010000218         4698 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.222, whole genome shotgun sequence.
ACCESSION   DAHHTG010000218 DAHHTG010000000
VERSION     DAHHTG010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4698)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4698)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4698
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.222"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            366..1589
                     /locus_tag="NBI74_004406"
     CDS             366..1589
                     /locus_tag="NBI74_004406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001362826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HCD1934631.1"
                     /translation="MGTIQKIRTDVNVLHAAIHRIEWLFETFSSVCLSFSGGKDSTVL
                     LHLTADVARRKKRRFSVLFIDWEAQYQCTIAHILKMREMYRDVTETFYWVALPLTTVN
                     GVSQFQPEWICWEPGVEWVRQPPDDAITDMSYFPFYRYAMTFEEFVPAFSSWFAGNRC
                     GVAILTGVRADESLNRFMGLVSQRKLRYADDKPWTTASPEGFYYTLYPLYDWKARDIW
                     IYNARTRAIYNPLYDLMYRAGVPLRNMRVCEPFGPEQRKGLWLYHVLEPETWARMCER
                     VSGAASGALYANESGAYFALRKRITKPPHHTWRSYAMFLLDVMPERTAEHYRNKIAVY
                     LRWYQTRGFPDDIPDEQENDLGSRDIPSWRRICKTLIKNDFWCRTLSFSPNKPRHYER
                     YLQRMKERRKEWGIL"
     gene            1574..2218
                     /locus_tag="NBI74_004407"
     CDS             1574..2218
                     /locus_tag="NBI74_004407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB N-terminal domain-containing protein"
                     /protein_id="HCD1934632.1"
                     /translation="MGDSVTPEVEVLSNMIRQYFSQERSEEETIRALNHLRRVLHEVS
                     PFAQEPVDCVLWVKADEVVANDYNPNVMSSSEKKLLKHSLEQDGFTQPVVVSEEKEHY
                     LVVDGFHRQLLGRKADTRKRLKGWLPVTCINPERKGQASRIAATIRHNRARGKHQITS
                     MSDIVRDLSRLGWTDERIGTELGMDQDEVLRLKQISGLTELFQEDNFSPSWTVR"
     gene            complement(2589..4694)
                     /locus_tag="NBI74_004408"
     CDS             complement(2589..4694)
                     /locus_tag="NBI74_004408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015840948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HCD1934633.1"
                     /translation="MSLCISGIVSTATATSSETKISNEETLVVTTNRSASNLWESPAT
                     IQVIDQQTLQNSTNASIADNLQDIPGVEITDNSLAGRKQIRIRGEASSRVLILIDGQE
                     VTYQRAGDNYGVGLLIDESALERVEVVKGPYSVLYGSQAIGGIVNFITKKGGDKLASG
                     VVKAVYNSATAGWEESIAVQGSIGGFDYRINGSYSDQGNRDTPDGRLPNTNYRNNSQG
                     VWLGYNSGNHRFGLSLDRYRLATQTYYEDPDGSYEAFSVKIPKLEREKVGVFYDTDVD
                     GDYLKKIHFDAYEQTIQRQFANEVKTTQPVPSPMIQALTVHNKTDTHDKQYTQAVTLQ
                     SHFSLPANNELVTGAQYKQDRVSQRSGGMTSSKSLTGFINKETRTRSYYESEQSTVSL
                     FAQNDWRFADHWTWTMGVRQYWLSSKLTRGDGVSYTAGIISDTSLARESASDHEMVTS
                     TSLRYSGFDNLELRAAFAQGYVFPTLSQLFMQTSAGGSVTYGNPDLKAEHSNNFELGA
                     RYNGNQWLIDSAVYYSEAKDYIASLICDGSIVCNGNTNSSRSSYYYYDNIDRAKTWGL
                     EISAEYNGWVFSPYISGNLIRRQYETSTLKTTNTGEPAINGRIGLKHTLVMGQANIIS
                     DVFIRAASSAKDDSNGTETNVPGWATLNFAVNTEFGNEDQYRINLALNNLTDKRYRTA
                     HETIPAAGFNAAIGFVWNF"
BASE COUNT         1221 a          990 c         1148 g         1339 t
ORIGIN      
        1 gatattctct ggataatgat tcaagagttt aagttcattg tatatataag tttgtattca
       61 aaggtaataa attccttgtt tattatttct catgaaaata gttaagaagg agtgaagagt
      121 ctgttttttt ggtaggagag tctttattta tgtgtttgct attaatgaat ttttgttaag
      181 gtagcctcat ttgtcataat tttgctaata tatgtatcgt catatgtcgt gttttactaa
      241 aaacttaact atcatgaact cgtgatagta acaatatacc cttaaggtag aactttttct
      301 tgatgagaag gtttaagttt gtagttcaga atattactgc caagatagtt tatactagga
      361 tatgtatggg aaccatacag aagataagaa cagatgttaa tgtattacat gcggccattc
      421 accgtattga gtggttgttt gagacatttt catctgtttg tttgtctttt tctggaggaa
      481 aagactctac tgtgctgctc catcttacgg ccgatgtggc tcgcaggaag aaacgtcgtt
      541 tctctgtatt attcattgac tgggaagctc agtaccagtg cacaattgca cacattctga
      601 agatgaggga gatgtatcgg gatgtgacgg agacttttta ctgggtggca ctccccctga
      661 ctacggtaaa cggtgtctct cagtttcagc cggaatggat atgctgggaa ccaggtgtgg
      721 agtgggttcg tcagccacca gatgacgcta ttacagatat gtcgtatttc ccattttatc
      781 ggtatgccat gacgtttgaa gaatttgttc cggcattttc ttcctggttt gccggtaacc
      841 ggtgtggagt ggcaatactg actggtgttc gtgctgatga atcgctcaat cgctttatgg
      901 gactggtgtc tcagcgcaaa ctgagatatg cagatgataa accctggacc acagcgtcac
      961 ctgaagggtt ttattacacc ttgtatccgt tgtatgactg gaaagcccgt gatatatgga
     1021 tatataacgc cagaacccga gctatctaca atcccctgta tgacctgatg taccgtgccg
     1081 gcgtgccgtt acgcaacatg cgggtctgtg agccttttgg cccggaacag cgtaagggac
     1141 tgtggcttta ccatgttctg gagccggaaa cttgggccag gatgtgtgag cgggtgtcgg
     1201 gtgctgccag cggggcgctt tatgccaatg agagcggtgc ctattttgcc ctgcgtaagc
     1261 gtatcacgaa gccgcctcat catacctggc gtagctatgc gatgttcctg ctggatgtga
     1321 tgccggaaag aacggcagaa cattaccgta ataaaattgc tgtctacctg cgctggtatc
     1381 agacgcgggg cttcccggat gacatcccgg atgaacagga gaatgacctg gggagccggg
     1441 atatcccgtc ttggcgacgt atctgtaaga cactcataaa gaatgatttc tggtgccgga
     1501 ccctctcctt cagtccgaac aaaccccggc actatgaacg ttatctgcag cgtatgaaag
     1561 aaaggaggaa ggaatggggg attctgtgac acctgaggtg gaagtattga gcaacatgat
     1621 tcgacagtac ttcagtcagg aacgatctga agaagagacc atacgagccc ttaatcatct
     1681 gcgcagggtg ttgcatgagg tcagcccttt tgcacaggag cctgtggact gtgtgttgtg
     1741 ggtgaaagcg gatgaggtgg tggccaacga ttacaatccc aatgtcatgt cttccagtga
     1801 aaaaaaactg cttaaacatt cactggagca ggatggattc acgcagcctg tggtggtttc
     1861 ggaagagaag gagcattatc tggtcgtgga tggttttcat cgtcagttgc tgggcaggaa
     1921 agcggatacg agaaaacgtc tgaagggctg gctgccagtg acctgtatta atccggaaag
     1981 aaagggacag gcatcacgta ttgccgcaac catacggcat aaccgtgcca ggggaaaaca
     2041 ccagataacg tcaatgtctg acattgtcag ggacctttcg cgtctgggct ggacagatga
     2101 gcgtattggc acagagttgg gcatggacca ggatgaagtg ctgaggctga aacagattag
     2161 cgggctgaca gagctgtttc aggaagataa tttcagccct tcatggacgg ttcgttgata
     2221 ttgtaaaata tgatagttgg agtaaggggc aatagaagga gaatatttat cccaccttct
     2281 tccgagtcgg gataaatcaa aaggtattat ccattgattc agtatctggg tgagagtaaa
     2341 taatctaaaa atagtattgc gttgtgaact gatatcactt ttgaattaca aaggtatagg
     2401 ctcagtaacc agctttataa gtcattgtcg tagcaatgac tatagagtca tgctggcggg
     2461 aagaataaat attcaccccc agatggaaaa caacgtcgaa aaaccttcaa caaacacaat
     2521 acgttcgtta gcctgcccgc aaagcgggca gatcaggaca aagaattcca taacgattgt
     2581 ttttgtcatc agaaattcca tacaaaacct atagctgcat taaaacctgc tgcaggaata
     2641 gtttcatgtg ctgtacggta gcgtttgtct gtcaggttat tgagtgctag gttaatccgg
     2701 tactgatcct cgttaccgaa ttctgtattt actgcaaagt tgagagtggc ccagcccgga
     2761 acatttgttt cggtaccgtt actgtcatct tttgcactag aggcagcacg aataaaaaca
     2821 tcagagatta tgttggcctg acccatcaca agagtatgtt tcagccctat acgtccgttt
     2881 atcgctggtt ctcctgtatt agttgttttt aatgttgaag tttcatattg ccgacgaatt
     2941 aaattgccac tgatatatgg cgagaaaacc cagccattat attccgcgct tatttccagt
     3001 ccccatgttt ttgcccgatc aatattgtca taataatagt agctactacg ggaggagttg
     3061 gtgttaccat tgcaaactat actgccatca cagatcagac ttgcaatata atctttagct
     3121 tctgagtagt aaactgcgct gtcaatcagc cactgattac cattatatcg tgcacctaat
     3181 tcaaagttat tggagtgttc agccttaaga tcaggatttc cgtatgtgac actgccgccc
     3241 gcagatgtct gcataaaaag ctgggagagt gtgggaaata cgtagccttg cgcgaacgca
     3301 gcgcgtaact ccaagttatc gaaacctgaa tagcgcaggc ttgtagatgt taccatttcg
     3361 tgatcactcg cagactctct ggcaagagag gtatcgctta taatgcctgc ggtatatgat
     3421 actccgtcac cacgcgtcaa ctttgaagaa agccagtatt ggcgaactcc cattgtccat
     3481 gtccagtgat cggcgaatcg ccagtcattt tgtgcgaata gtgagactgt actttgctct
     3541 gactcataat aggagcgagt tcgtgtttcc ttattaatga agccggtcag agatttgctt
     3601 gaggtcatgc caccggacct ttggctgacc ctgtcttgtt tgtactgtgc accggtaaca
     3661 agttcattat tagcaggcag cgaaaagtga ctctgcaatg tgaccgcctg agtgtattgc
     3721 ttatcatggg tgtcagtctt gttatgaacg gtcagagcct gaatcatcgg actgggaaca
     3781 ggctgtgtcg tttttacttc gttggcaaat tggcgctgga tggtctgctc atacgcgtcg
     3841 aaatgaattt tttttagata gtcaccgtcc acgtctgtgt catagaatac cccaactttc
     3901 tctcgttcaa gtttaggtat tttgacacta aatgcctcat agcttccgtc tggatcctca
     3961 tagtaagttt gcgtcgcgag tctgtagcga tcaagcgaga ggccaaaacg atggtttccg
     4021 gagttataac ccaaccatac accctgacta ttgttacgat agttggtatt cggcagacgt
     4081 ccatccggcg tatcacgatt gccctgatca gaataactac cgttgatgcg ataatcaaat
     4141 ccaccgatgc tcccctggac cgcgattgat tcttcccagc ctgctgttgc ggaattataa
     4201 acagctttca caactccaga tgcaagtttg tcacctccct ttttggttat gaagttaaca
     4261 ataccgccaa ttgcctgtga accgtacagt acggaatatg gacctttcac tacctcaaca
     4321 cgctccagcg cagactcatc tatcaacagt cccacaccat aattatctcc ggcgcgctga
     4381 taagttacct cctgaccatc aatgagaatt aaaacacggg aggatgcttc gccacgaatg
     4441 cggatttgtt tacggcctgc caaggagttg tctgttatct ctactccggg gatgtcctgc
     4501 aaattatcgg ctatggaggc attggtggag ttctgcaatg tttgttggtc aataacctgt
     4561 atagtcgccg ggctttccca aaggttgctt gccgaacgat tcgtggtcac gacgagcgtc
     4621 tcttcgttgc tgatttttgt ttctgaagaa gttgcggttg cagtagaaac tatcccagaa
     4681 atgcataatg acagagtg
//
LOCUS       DAHHTG010000219         4635 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.223, whole genome shotgun sequence.
ACCESSION   DAHHTG010000219 DAHHTG010000000
VERSION     DAHHTG010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4635)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4635)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4635
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.223"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            252..1349
                     /gene="mltA"
                     /locus_tag="NBI74_004409"
     CDS             252..1349
                     /gene="mltA"
                     /locus_tag="NBI74_004409"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase A"
                     /protein_id="HCD1934634.1"
                     /translation="MKGRWVKYLLMGTVVAMLAACSSKPTDRGQQYKDGKFTQPFSLV
                     NQPDAVGAPINAGDFAEQINHIRNSSPRLYGNQSNVYNAVQEWLRAGGDTRNMRQFGI
                     DAWQMEGVDNYGNVQFTGYYTPVIQARHTRQGEFQYPIYRMPPKRGRLPSRAEIYAGA
                     LSDKYILAYSNSLMDNFIMDVQGSGYIDFGDGSPLNFFSYAGKNGHAYRSIGKVLIDR
                     GEVKKEDMSMQAIRHWGETHSEAEVRELLEQNPSFVFFKPQSFAPVKGASAVPLVGRA
                     SVASDRSIIPLGTTLLAEVPLLDNNGKFNGQYELRLMVALDVGGAIKGQHFDIYQGIG
                     PEAGHRAGWYNHYGRVWVLKTAPGAGNVFSG"
     gene            1426..2232
                     /gene="tcdA"
                     /locus_tag="NBI74_004410"
     CDS             1426..2232
                     /gene="tcdA"
                     /locus_tag="NBI74_004410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cyclic N6-threonylcarbamoyladenosine(37)
                     synthase TcdA"
                     /protein_id="HCD1934635.1"
                     /translation="MSVVISDAWRQRFGGTARLYGEKALQLFADAHICVVGIGGVGSW
                     AAEALARTGIGAITLIDMDDVCVTNTNRQIHALRDNVGLAKAEVMAERIRQINPECRV
                     TVVDDFVTPDNVAQYMNAGYSYVIDAIDSVRPKAALIAYCRRNKIPLVTTGGAGGQID
                     PTQIQVTDLAKTIQDPLAAKLRERLKSDFGVVKNSKGKLGVDCVFSTEALVYPQSDGT
                     VCAMKATAEGPKRMDCASGFGAATMVTATFGFVAVSHALKKMMAKAARQG"
     gene            complement(2283..2726)
                     /gene="csdE"
                     /locus_tag="NBI74_004411"
     CDS             complement(2283..2726)
                     /gene="csdE"
                     /locus_tag="NBI74_004411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase sulfur acceptor subunit
                     CsdE"
                     /protein_id="HCD1934636.1"
                     /translation="MTNPQFAGHPFGTTVTAETLRNTFAPLTQWEDKYRQLIMLGKQL
                     PALPEELKAQAKEIAGCENRVWLGYTVAENGKMHFFGDSEGRIVRGLLAVLLTAVEGK
                     TAAELQAQSPLALFDELGLRAQLSASRSQGLNALSEAIIAAAKQV"
     gene            complement(2726..3931)
                     /gene="csdA"
                     /locus_tag="NBI74_004412"
     CDS             complement(2726..3931)
                     /gene="csdA"
                     /locus_tag="NBI74_004412"
                     /EC_number="3.13.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase CsdA"
                     /protein_id="HCD1934637.1"
                     /translation="MNVFNPAQFRAQFPALQDAGVYLDSAATALKPEAVVEASRQFYS
                     LSAGNVHRSQFAEAQRLTARYEAAREKVAQLLNASDDKNIVWTRGTTESINMVAQCYA
                     RPRLQPGDEIIVSVAEHHANLVPWLMVAQQTGAKVVKLPLNAQRLPDVDLLPELITPR
                     SRILALGQMSNVTGGCPDLARAITFAHSAGMVVMVDGAQGAVHFPADVQQLDIDFYAF
                     SGHKLYGPTGIGVLYGKPELLEAMSPWLGGGKMVHEVSFDGFTTQSAPWKLEAGTPNV
                     AGVIGLSAALEWLTDYDINQAESWSRSLATLAEEALAKRPGFRSFRCQDSSLLAFDFA
                     GVHHSDMVTLLAEYGIALRAGQHCAQPLLAELGVTGTLRASFAPYNTKSDVDALVNAV
                     DRALELLVD"
     gene            4123..4350
                     /locus_tag="NBI74_004413"
     CDS             4123..4350
                     /locus_tag="NBI74_004413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="HCD1934638.1"
                     /translation="MKKTAAIISACMLTFALSACSGSNYVMHTNDGRTIVSDGKPQTD
                     NDTGMISYKDANGNKQQINRTDVKEMVELDQ"
BASE COUNT         1106 a         1176 c         1244 g         1109 t
ORIGIN      
        1 cgggttatga gcccgacgag ctaccaggct gctccacccc gcgtccgtgg atgcgcacta
       61 tactctgctc gcgttttcat gcaacctttt ttttaccccg atcacggatt tggcatgatt
      121 ttgaacaaaa acaaatcact aaagcctatt ttttgtgcaa aatttgcgtc agggagaggc
      181 tgctcatttc attaaacgcg gtggtttgtt atcttcgttg cgccttattt tttaacctga
      241 agaagagaac aatgaaagga cgttgggtaa agtaccttct tatgggcacg gttgtggcaa
      301 tgcttgccgc ctgctcttcc aaaccaaccg atcgcggaca gcaatataaa gacgggaaat
      361 ttacccagcc tttctctctg gtgaaccagc cagatgccgt tggcgcgccg attaatgccg
      421 gtgattttgc cgagcaaatt aaccatatcc gtaattcgtc accgcgtctg tatggcaacc
      481 agagtaatgt ttataacgcg gtgcaagagt ggctgcgcgc aggcggtgat acccgcaata
      541 tgcgccagtt cggcattgat gcctggcaga tggaaggtgt cgacaactat ggtaacgtgc
      601 agtttacagg ctattacacg ccggtaattc aggcgcgcca tacccgtcag ggcgagttcc
      661 agtatccgat ttaccgtatg ccgccaaaac gtggtcgtct gccgtctcgt gcggagatct
      721 acgcaggagc gctgagtgat aaatatattc tcgcttacag taactccctg atggataact
      781 tcattatgga tgtgcagggt agcgggtata tcgactttgg tgatggcagt ccgcttaact
      841 ttttcagcta tgcagggaaa aacggtcatg cctatcgcag cattggtaag gtgttgatcg
      901 accgtggcga agtgaaaaaa gaagatatgt cgatgcaggc gattcgtcac tggggcgaaa
      961 cacacagtga agctgaggtt cgcgagctgc tggaacagaa cccgtctttc gtcttcttta
     1021 aaccgcaatc ttttgcaccg gtgaaagggg caagtgcggt gccgctggtt ggccgtgctt
     1081 ctgttgcctc cgatcgttca attattccgc taggtactac cttgctggca gaagtgccgc
     1141 tgctggataa taacggcaaa tttaacggtc agtacgaatt gcgtctgatg gtggcgctgg
     1201 atgtcggtgg cgcaatcaaa ggccaacact tcgatatcta tcaagggatc gggccggaag
     1261 ccggacaccg cgcaggttgg tacaaccact atggacgtgt ctgggtgctg aaaaccgccc
     1321 cgggcgcagg taacgtcttt agcggctgat gtgatattct gtatgccgtt tttacggata
     1381 tgacagggtg aggggtaacc tcaccctttt taattctgag gtgctatgtc tgtggtaatt
     1441 agtgatgcat ggcgtcagcg ttttggtggc acagcgcgtc tgtatggtga aaaagcattg
     1501 caactgtttg ctgacgcgca tatttgtgtg gttggtatcg gtggtgtcgg ttcctgggcg
     1561 gcggaagcgc tagcgcgcac ggggattggt gcaatcacgc ttatcgatat ggatgatgtg
     1621 tgcgtcacca ataccaatcg gcaaattcac gctctgcgcg ataacgtcgg gctggcaaaa
     1681 gcggaagtca tggcggagcg tattcgccag attaacccgg agtgccgtgt aacggtagtg
     1741 gatgacttcg tgacgccgga taacgtagcg caatatatga atgccggtta ttcgtacgtg
     1801 attgatgcta ttgatagtgt acggcccaaa gcggcgctga ttgcttattg tcggcgcaat
     1861 aaaatcccgc tggtcacgac cggtggcgct ggtgggcaga ttgacccgac gcagattcag
     1921 gttaccgatc tggcgaaaac gattcaggac ccgttggcgg cgaagttgcg cgagcgcctg
     1981 aaaagcgatt ttggcgtagt gaaaaacagt aaaggtaagc tcggcgtgga ttgcgtgttc
     2041 tctaccgaag cgctggtgta cccgcagtca gacggcaccg tctgtgcgat gaaagccacg
     2101 gcggaagggc caaagcggat ggattgtgcc tcaggatttg gcgcggcgac gatggtgacc
     2161 gccacttttg gctttgttgc ggtttctcat gcgctgaaga agatgatggc gaaagcggcg
     2221 cgtcaggggt aaacaatcag gtcggatgcg acgcgaacgt cttatccgac ctggttctgt
     2281 gcttaaacct gcttcgccgc agcgataatc gcctcgctta acgcattcaa cccctggctg
     2341 cgtgaggcgc taagttgcgc acgtaatccc agctcatcaa acaatgccag cggcgactgt
     2401 gcctgcaatt cggcagcggt tttcccctca acggcagtca acaacaccgc caacaggccg
     2461 cgcacaatgc gcccttcgct gtcgccaaag aaatgcatct tgccgttttc agccacggta
     2521 tatcccagcc agacgcggtt ttcgcatccg gcaatctctt tagcctgcgc ttttaactcc
     2581 tctggcaaag ccggaagctg tttccccagc atgatcaact gccgatattt atcttcccat
     2641 tgcgtcaacg gtgcgaaggt attgcgtaac gtttctgcgg ttacggttgt gccgaacgga
     2701 tgtccggcga attgcgggtt tgtcattaat ccaccaataa ttccagcgcg cggtcaacgg
     2761 cattcaccag cgcatccaca tcactctttg tattatatgg cgcaaatgag gcgcgcagtg
     2821 tgccggttac gcctaattct gccagtagcg gctgagcgca atgctgcccg gcgcgcaggg
     2881 caataccgta ctccgccagc agtgtcacca tatcgctgtg atgaactcca gcaaaatcaa
     2941 aggccagcag gctggaatcc tggcagcgga atgaacgaaa gcctggacgt ttcgccagcg
     3001 cttcttctgc aagcgttgct aagctacggc tccagctttc ggcttggttg atatcgtaat
     3061 ctgtcagcca ttccagcgcc gcgcttaatc ctatgacgcc agcgacattt ggcgttcctg
     3121 cttccagttt ccacggcgca gattgagtcg tgaaaccgtc aaaactcact tcgtgaacca
     3181 ttttgccgcc gcccagccag ggcgacatcg cttccagcag ttctggtttg ccatacagca
     3241 cgccgatgcc tgtcggacca tacagtttgt gacctgaaaa agcatagaaa tcaatatcca
     3301 gttgctgaac atccgcgggg aaatgcaccg ccccctgagc accatcaacc atcaccacca
     3361 tcccggctga atgagcaaaa gtaatcgctc tcgccagatc cgggcaaccg ccagtgacgt
     3421 tcgacatctg gcccaacgcc agaatccgac tacggggagt aatcagttct ggcaacaaat
     3481 cgacatccgg cagtcgctgc gcattaagcg gcaatttcac cactttggcc cccgtttgtt
     3541 gggcgaccat cagccagggg acgaggttgg cgtggtgttc tgccacactg acaataattt
     3601 catcgcccgg ttgcagacgc ggacgggcat agcattgcgc caccatgttg atggattcag
     3661 tggtgccgcg cgtccagacg atatttttgt catccgatgc attcagtaat tgtgccactt
     3721 tttcccgcgc agcttcgtaa cgcgcggtca ggcgttgggc ttcggcaaac tggctgcgat
     3781 ggacgtttcc ggcgctcagg ctataaaact gtcgggaggc ttcaaccacg gcttcaggtt
     3841 taagcgcggt cgcggcgctg tcgagataga cgcccgcatc ctgtagcgcg ggaaactggg
     3901 cgcgaaactg cgcgggatta aaaacgttca tggtactcct cggcttgatg caccgatcgt
     3961 gacgcaaaat tcactttgat acaaggagag tgctaaccat cagaattatc tggatatcct
     4021 ggcgaagccg cgtcagtaac atatgctgaa tagtgttagg cgttaatttt agcctgttaa
     4081 tcaatactat taagatttat taatcgcttt aaggagaaga tgatgaaaaa gactgccgca
     4141 attatttctg cctgtatgct gacttttgcc ctgagcgcct gttccggttc gaactatgtg
     4201 atgcacacca atgacggacg taccatcgtc tctgacggca aaccacagac tgataacgat
     4261 accggtatga tttcgtataa agacgctaat ggcaacaaac agcagatcaa ccgtaccgac
     4321 gtgaaagaga tggtcgaact ggatcagtaa ttcgcgatcg caaggtaaaa aaaaagcacc
     4381 gcaattaggc ggtgctacat taatcactat ggacagacag ggtaaatgta caggaagtga
     4441 aaaaaggtag ctttgctacc atggtctgaa tcgcagacca attgcaaaca caacaacaca
     4501 acatcacaac cgtaagccaa aagttcacca gaacacgcat tccgataaaa cttttcgttc
     4561 cggctcagga agtgccgcca ctataggtat ttgctggtag aagctcaacg gacaatttat
     4621 aatggctcag attaa
//
LOCUS       DAHHTG010000220         4623 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.224, whole genome shotgun sequence.
ACCESSION   DAHHTG010000220 DAHHTG010000000
VERSION     DAHHTG010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4623)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4623)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4623
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.224"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            327..2750
                     /gene="pgaA"
                     /locus_tag="NBI74_004414"
     CDS             327..2750
                     /gene="pgaA"
                     /locus_tag="NBI74_004414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6 N-acetyl-D-glucosamine export
                     porin PgaA"
                     /protein_id="HCD1934639.1"
                     /translation="MYSSSRKRCPKTKWALKILTAAFLAASPAAKSAVNNAYDALIIE
                     ARKGNTQPALSWFAQKSTLSNNQIADWLQIALWAGQDKQVITVYNRYRHQQLPARGYA
                     AVAVAYRNLQQWQNSLTLWQKALSLEPQNKDYQRGQILTLADAGHYDTALFKLKQLKS
                     GAPDKANLLAEAYIYKLTGRYQDELRAMTESLPENASKQQYPTEYVLALRNNQLAAAI
                     DDANLTPDIRADIHAELVRLSFMPTRSENERYAIADRALAQYAALEILWHDNPDRTAQ
                     YQRIQVDHLGALLTRDRYRDVISHYQRLKKTGQIIPPWALYWVASAYLKDQQPKKAQS
                     IMTELFYNKETIATDLSDEELADLFYSHLESENYPGALTVTKHTINTSPPFLRLMGTP
                     TSIPNDTWLQGHSFLSTVAKYSNDLPQAEITTRELAYNAPGNQGLRIDYASVLQARGW
                     PRAAENELKKAEVIEPRNINLEVEQAWTALTLQEWQQAAVLTHDVVEREPQDPGVVRL
                     KRAVDVHNLAELRIAGSTGIDAEGPDSGKHDVDLTTIVYSPPLNDNWRGFAGFGYADG
                     QFSEGKGIVRDWLAGVEWRSRNIWLEAEYAERFFNHEHKPGARLSGWYDFNDNWRIGS
                     QLERLSHRVPLRAMKNGVTGNSAQAYVRWYQNERRKYGVSWAFTDFSDSNQRHEVSIE
                     GQERIWSSPYLIVDFLPNLYYEQNTEHDTPYYNPIKMFDIVPAFEASHLLWRSYENSW
                     EQIFSAGVGASWQKHYGTDVVTQLGYGQRISWNDVIDAGATLRWEKRPYDGDREHNLY
                     VEFDMTFRF"
     gene            2759..>4623
                     /gene="pgaB"
                     /locus_tag="NBI74_004415"
     CDS             2759..>4623
                     /gene="pgaB"
                     /locus_tag="NBI74_004415"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6-N-acetyl-D-glucosamine
                     N-deacetylase PgaB"
                     /protein_id="HCD1934640.1"
                     /translation="MLRNGNKYLLMLVSIIMLTACISQSRTSFIPPQDRESLLAEQPW
                     PHNGFVVISWHNVEDEAADQRFMSVRTSALREQFAWLRENGYQPVSIAQIREAHRGGK
                     PLPEKAVVLTFDDGYQSFYTRVFPILQAFQWPAVWAPVGSWVDTPADKQVKFGDEMVS
                     REYFATWQQVREVARSRLVEVASHTWDSHYGIQANATGSLLPAYVNRAYFTDHARYET
                     AAEYRERIRLDAVKMTEYLRTKAEVNPHVFVWPYGEANGIAIAELKKLGYDMFFTLES
                     GLANASQLDSIPRVLIANNPSLKEFAQQIITVQEKPLQRVMHIDLDYVYDENRQQMDR
                     NIDVLIQRVKDMQISTVYLQAFADPDGDGLVKEVWFPNRLLPMKADIFSRVAWQLRTR
                     SGVNIYAWMPVLSWDLDPTLTRVKYLPTGEKKAQIHPEQYRRLSPFDDRVRAQVGMLY
                     DDLAGHAAFDGILFHDDALLSDYEDASAAAIAAYQQAGFSGSLSEIRQNPEQFKQWTR
                     FKSRALADFTLELSARVKAIRGPHVKTARNIFALPVIQPESEAWFAQNYADFLKSYDW
                     TAIMAMPYMEGVTEKSADQWLIQLTNQMKSIPQAKDKSILELQAQNWQKNGQHQA"
BASE COUNT         1324 a         1024 c         1063 g         1212 t
ORIGIN      
        1 agcgctgcct ttcatcagga ctttcgaaaa atccgaaatc atgcatcgga atttactgat
       61 ttaattattt taatcctaat ttattttgaa aaaggtattg ggatttatgc cgtattcctg
      121 aagatcctca tcattggaat ggattttcgg gcgagaaaag gattttatat ggacactctg
      181 ctcatcattt cttcttctca tcatcaacaa ttcacgtctc tcttccgcgt ttaataacgg
      241 attatgagat gcaaaaatat ctttcttttc agttacctgt aattagatac agagagagat
      301 tttggcaata catggagtaa tacaggatgt attcaagtag cagaaaaagg tgcccgaaaa
      361 ccaaatgggc tttgaaaatt cttactgctg catttttagc agcgagtccc gcggcgaaga
      421 gtgctgttaa taacgcctat gatgcattga ttattgaagc tcgcaaagga aatactcagc
      481 cagctttgtc atggtttgca caaaaatcaa cactcagtaa taaccaaatt gctgactggt
      541 tacagattgc cttatgggcc gggcaagata aacaggttat taccgtttac aaccgctatc
      601 gtcatcagca attaccagcg cgtggttatg cagctgtcgc cgtcgcttat cgtaacctgc
      661 aacaatggca aaactcgctt acactgtggc aaaaggcgct ctctctggag ccgcaaaata
      721 aggattatca acggggacaa attttaaccc tggcagatgc gggtcactat gatactgcgc
      781 tgtttaaact taagcagctt aaatctggag caccggacaa agccaattta ctcgcagaag
      841 cctatatcta taaactgacg gggcgttatc aggatgaatt acgggcgatg acagagtcat
      901 tacctgaaaa tgcatctaag caacaatatc ccacagaata cgtgctggca ttacgtaata
      961 atcaactagc tgccgcgatt gacgatgcca atttaacgcc agatattcgc gctgatattc
     1021 atgccgaact ggtcagactg tcgtttatgc ctacgcgcag tgaaaatgaa cgttatgcca
     1081 ttgccgatcg cgccctcgcc caatacgctg cattagaaat tctgtggcac gataacccag
     1141 accgcactgc ccagtaccag cgtattcagg ttgatcatct tggcgcgtta ttaactcgcg
     1201 atcgttatag agacgttatt tctcactatc agcgtttaaa aaagacgggg caaattattc
     1261 cgccctgggc gctatattgg gttgcgtcgg cttatctcaa agatcagcag ccgaaaaaag
     1321 cacagtcaat aatgaccgag ctcttttata acaaggaaac cattgccacg gatttatccg
     1381 atgaagaact tgcggatctc ttttacagcc acctggagag tgaaaattat cccggcgcgc
     1441 taactgtcac caaacatacc attaatactt cgccgccttt ccttcggcta atgggcacgc
     1501 ctacgagcat cccgaatgat acctggttac aggggcattc gtttctctca accgtagcaa
     1561 aatatagtaa tgatcttcct caagctgaaa taacaaccag agaacttgct tataacgcac
     1621 caggaaatca gggactgcgc atcgattacg cgagtgtgtt acaagcccgc ggttggcctc
     1681 gtgcggcaga aaatgaatta aaaaaagcag aagtgatcga gccacgtaat attaatctgg
     1741 aggttgaaca agcctggaca gcattaacgt tacaagaatg gcagcaggca gctgtcttaa
     1801 cccacgatgt tgtcgaacgt gaaccgcaag atcccggcgt tgtacgatta aaacgtgcgg
     1861 ttgatgtaca taatcttgca gagcttcgta tcgctggctc aacaggaatt gatgccgaag
     1921 gtccagatag tggcaaacat gatgtcgact taaccaccat cgtttattca ccaccgctga
     1981 atgataactg gcgcggtttt gctggattcg gttatgccga tggacaattt agcgaaggaa
     2041 aagggattgt tcgcgactgg ctggcaggtg ttgagtggcg gtcacgtaat atctggctcg
     2101 aggcggagta cgctgaacgc tttttcaatc atgagcataa acccggcgcg cgcctgtctg
     2161 gctggtatga ttttaatgat aactggcgta ttggttcgca actggaacgc ctctctcacc
     2221 gcgttccatt acgggcaatg aaaaatggtg ttacaggcaa cagcgcacag gcttatgttc
     2281 gctggtatca aaatgagcgg cgtaagtacg gtgtctcctg ggctttcact gatttttccg
     2341 acagtaacca gcgtcatgaa gtctcaattg agggtcaaga acgcatctgg tcttcaccat
     2401 atttgattgt cgatttccta cccaatctgt attacgaaca aaatacagaa cacgacaccc
     2461 catactacaa ccctataaaa atgttcgata ttgttccggc atttgaggca agtcatttgt
     2521 tatggcgaag ctatgaaaat agctgggagc aaatattcag cgcaggtgtt ggtgcctcct
     2581 ggcaaaaaca ttatggcacg gatgtcgtca cccaactcgg ctacgggcaa cgtattagct
     2641 ggaatgacgt gattgatgct ggcgcaacgc tacgctggga aaaacgacct tatgacggtg
     2701 acagagaaca caacttatac gttgaattcg atatgacatt cagattttaa ggataaatat
     2761 gttacgtaat ggaaataaat atctcctgat gctggtgagt ataattatgc tcaccgcgtg
     2821 cattagccag tcaagaacat catttatacc gccacaggat cgcgaatctt tactcgccga
     2881 gcaaccgtgg ccacataatg gttttgtggt gatttcatgg cataacgttg aagacgaagc
     2941 tgccgaccag cgttttatgt cagtgcgaac atcagcactg cgtgaacaat ttgcctggct
     3001 gcgcgagaac ggttatcaac cggtcagtat tgctcaaatt cgtgaagcac atcgaggagg
     3061 aaaaccgcta ccggaaaaag cagtagtgct gacttttgat gacggctacc agagttttta
     3121 tacccgtgtc ttcccaattc ttcaggcctt ccagtggccg gctgtatggg ctcccgtcgg
     3181 cagttgggtc gatactccag cggataaaca agtgaaattt ggcgatgaga tggtcagtcg
     3241 agaatatttt gccacgtggc aacaagtgcg agaagttgcg cgttcccggc tcgttgaggt
     3301 cgcttctcat acatgggatt ctcactacgg tattcaggct aatgccaccg gcagcttatt
     3361 gcctgcatat gtaaatcgtg catattttac tgaccacgca cggtatgaaa ccgcagcaga
     3421 ataccgggaa agaattcgcc tggatgctgt aaaaatgacg gaatacctgc gtacaaaggc
     3481 tgaggtaaat ccacacgttt ttgtttggcc ttatggcgaa gcgaatggca tagcaatagc
     3541 ggaattaaaa aaactcggtt atgacatgtt cttcaccctt gaatcaggtt tggcaaatgc
     3601 gtcgcaatta gattccattc cgcgagtatt aatcgccaat aatccctcat taaaagagtt
     3661 tgcccagcaa attattaccg tacaggaaaa accactacaa cgggtaatgc atatcgatct
     3721 tgattacgtt tatgacgaaa accgccagca aatggatcgc aatattgatg tgctaattca
     3781 gcgggtaaaa gatatgcaaa tatctaccgt gtatttgcaa gcatttgctg atcccgatgg
     3841 tgatgggctg gttaaagagg tctggtttcc aaatcgtttg ctaccaatga aagcagatat
     3901 ttttagtcgg gttgcctggc aattgcgtac ccgctcaggt gtaaacatct atgcgtggat
     3961 gccggtatta agctgggatt tagatcccac attaacgcga gtaaaatact taccaactgg
     4021 ggagaaaaaa gcacaaattc atcctgaaca ataccgccgt ctctctcctt tcgatgacag
     4081 ggtcagagca caagttggta tgttatatga tgatcttgcc ggacatgctg cttttgatgg
     4141 catattgttc cacgatgatg ctttgctttc agattatgaa gatgccagtg cagcggctat
     4201 cgcggcttat cagcaagctg gctttagcgg gagtctgagc gaaattcgac aaaacccgga
     4261 gcaatttaaa cagtggaccc gctttaaaag tcgtgcgtta gctgacttca ctttagaact
     4321 tagtgcgcgc gtaaaagcca ttcgtggtcc acatgttaaa actgcacgaa atatttttgc
     4381 acttccggta atacaacctg aaagtgaagc ctggtttgca cagaattatg ctgatttcct
     4441 aaaaagctat gactggaccg ctattatggc tatgccttat atggaaggcg tcacagaaaa
     4501 atcggctgat caatggttaa tacaattgac caatcagatg aaaagcatcc cgcaggctaa
     4561 agacaaatca attttagaat tacaggcaca aaactggcag aaaaatggtc agcatcaggc
     4621 tat
//
LOCUS       DAHHTG010000221         4621 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.225, whole genome shotgun sequence.
ACCESSION   DAHHTG010000221 DAHHTG010000000
VERSION     DAHHTG010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4621)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4621)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4621
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.225"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            189..899
                     /locus_tag="NBI74_004416"
     CDS             189..899
                     /locus_tag="NBI74_004416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HCD1934641.1"
                     /translation="MQTTRTPYSVSFMATVLLLFLFACHSTIANAAVALGATRVIYPA
                     NQKQVLLPVTNNDPASVYLIQSWIENAGDQKDTQFVITPPLFSMQGKKENTLRIINAT
                     NHQLPGDRESLFWVNVKAIPAMEKDQKNENTLQLAIISRIKMFYRPTHLAMAPEEAPA
                     MLRFRRSGSILTLINPTPYFITVTNMKAGNSNLPNTMVPPKGEVSVDIPHAVTGDISF
                     QTINDYGALTPRIKATMQ"
     gene            941..3592
                     /locus_tag="NBI74_004417"
     CDS             941..3592
                     /locus_tag="NBI74_004417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HCD1934642.1"
                     /translation="MTAFRAAFKAYRMHQVLILPRFARLTFALGLATAVFPVDAEYYF
                     NPRFLSNDLAESVDLSAFTKGREAPPGTYRVDIYLNDEFMASRDITFIADDNNADLIP
                     CLSTDLLVSLGIKKSALLDNKEHSADKHVPDNSACTPLQDRLADASSEFDVGQQHLSL
                     SVPQIYVGRMARGYVSPDLWEEGINAGLLNYSFNGNSINNRSNHNAGKSNYAYLNLQS
                     GINIGSWRLRDNSTWSYNSGSSNSSDSNKWQHINTSAERDIIPLRSRLTVGDSYTDGD
                     IFDSVNFRGLKINSTEAMLPDSQHGFAPVIHGIARGTAQVSVKQNGYDVYQTTVPPGP
                     FTIDDINSAANGGDLQVTIKEADGSIQTLYVPYSSVPVLQRAGYTRYALAMGEYRSGN
                     NLQSSPKFVQASLMHGLKGNWTPYGGMQIAEDYQAFNLGIGKDLGLFGAFSFDITQAN
                     TTLADDTRHSGQSVKSVYSKSFYQTGTNIQVAGYRYSTQGFYNLSDSAYSRMSGYTVK
                     PPTGDTSEQTLFIDYFNLFYSKRGQEQISISQQLGHYGTTFFSASRQSYWNTSRSDQQ
                     ISFGLNVPLGDITTSLNYSYSNNIWQNDRDHLLAFTLNVPFSHWMRTDSQSAFRNSNA
                     SYSMSNDLKGGMTNLSGVYGTLLPDNNLNYSVQVGNTQGGNTSSGTSGYSSLNYRGAY
                     GNTNVGYSRSGDSSQIYYGMSGGIIAHADGITFGQPLGDTMVLVKAPGADNVKIENQT
                     GIHTDWRGYAILPFATEYRENRVALNANSLADNVELDETVVTVIPTHGAIARATFNAQ
                     IGGKVLMTLKYGNKSVPFGAIVTHGENKNGSIVAENGQVYLTGLPQSGKLQVSWGNDK
                     NSNCIVDYKLPAVSPGTLLNQQTAICR"
     gene            3606..4136
                     /locus_tag="NBI74_004418"
     CDS             3606..4136
                     /locus_tag="NBI74_004418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCD1934643.1"
                     /translation="MKYNNVIFLGLCLGLTTYSALSADSVIKISGRVLDYGCTVSSDS
                     LNFTVDLQKNSARQFPTTGSTSPAVPFQITLSECSKGTTGVRVAFNGIEDAENNTLLK
                     LDEGSNTASGLGIEILDGNMRPVKLNDLHAGMQWIPLVPEQNNILPYSARMKSTQKSV
                     NPGLVSASATFTLEFQ"
     gene            4149..>4621
                     /locus_tag="NBI74_004419"
     CDS             4149..>4621
                     /locus_tag="NBI74_004419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCD1934644.1"
                     /translation="MKRLHKRFLLATFCTLFTATLQAADVTITVNGRVVAKPCTIQTK
                     EANVNLGDLYTRNLQQPGSASGWHNITLSLTDCPIETSAVTAIVTGSTDNTGYYKNEG
                     TAENIQIELRDDQDATLKNGDSKTVIVDEITRNAQFPLKARAITVNGNASQGTIEA"
BASE COUNT         1334 a         1017 c         1019 g         1251 t
ORIGIN      
        1 gttaaacttg ctgttattta aaatatttac ttggtagcaa ggttttccta tcaaaagaga
       61 accaacattt attacaaacg cattaagtct ggaaactcgg cgactcgcca atgtattagc
      121 aatatttacc gattgtctga tgtaacggtt tccccgctta tcctcttaac acatggagaa
      181 tgcatcagat gcaaactacg cgtaccccct actctgtttc ctttatggcg acagtgttac
      241 ttttatttct ctttgcttgc catagcacca ttgccaacgc agctgtcgca cttggtgcga
      301 cgcgggtaat ttatccggca aaccagaaac aggttttatt gcctgtcacg aataatgatc
      361 ctgccagtgt ctatctcatt cagtcatgga tagaaaatgc gggtgaccag aaagataccc
      421 agtttgtcat taccccaccg cttttttcca tgcaaggtaa aaaagagaat acgcttcgga
      481 taatcaacgc cactaatcat cagttaccag gcgaccgtga aagcctgttc tgggttaatg
      541 ttaaagctat ccctgctatg gaaaaagacc agaagaatga gaatacatta cagctggcaa
      601 ttattagccg cataaaaatg ttttatcgcc ccacgcatct ggcaatggca cctgaagaag
      661 cgcctgcaat gcttcggttt cgtcgcagcg gtagcatact cactcttatt aatccaacgc
      721 cctacttcat taccgtaaca aatatgaaag caggaaatag caatctccca aacaccatgg
      781 tgccgccaaa aggtgaagta tcggtggata tcccgcatgc tgtaaccggg gatatcagtt
      841 ttcagacaat caatgattac ggtgcattaa ctccgcgtat taaggcaacc atgcagtaag
      901 ccagtgacct aattaataat aaactcaatc gggattaaaa atgacagcct ttcgtgccgc
      961 atttaaggca tatcgtatgc atcaggtgtt aatactgcct cgctttgcac ggcttacctt
     1021 tgcccttggc ttagcaactg cggtttttcc cgttgacgca gagtattatt ttaatccacg
     1081 gtttttaagc aatgaccttg ctgaatctgt cgatttatct gcctttacta agggacgcga
     1141 ggccccacct ggtacatatc gggtcgacat ttatttgaac gacgaattta tggctagtcg
     1201 ggatattact tttattgcag atgataataa cgcagatctc atcccatgcc tgagtacaga
     1261 ccttcttgtc agtcttggca taaaaaaatc agcattatta gacaacaagg aacattcggc
     1321 agataaacat gtgccggaca acagcgcatg cacaccactt caggatcgtt tggctgacgc
     1381 atcatctgag tttgatgttg gtcagcaaca tctgtcactc tccgttccgc aaatatatgt
     1441 cggcagaatg gctcgcggct atgtttctcc tgatctgtgg gaagaaggaa taaatgccgg
     1501 gctactaaac tatagtttta atggtaattc tattaataac cgtagtaacc ataatgcagg
     1561 aaaatccaac tatgcatatt tgaatttaca gagtggcatc aacattggta gttggcgact
     1621 gcgcgataac tcaacgtgga gttataacag tgggagtagc aattcatctg acagcaataa
     1681 atggcagcat atcaatacgt cggctgaacg tgacattatt ccgttacgct cacggttaac
     1741 agtgggtgat agttataccg atggagatat ttttgacagt gtgaactttc gtggccttaa
     1801 aataaattca acagaagcga tgttgcccga tagccaacat ggtttcgctc cggtgattca
     1861 tggtattgct cgtggcaccg cacaagtgag tgtaaaacag aatggatacg atgtttatca
     1921 gactactgtc ccacccggcc cttttactat tgatgatatc aactctgcgg ccaatggtgg
     1981 tgatctgcaa gtaaccataa aagaggcaga cggcagtatt cagacattat atgttcctta
     2041 ttcgtctgtt ccggttctcc aacgtgctgg atatacgcgt tatgcacttg ccatggggga
     2101 atatcgtagt ggaaataacc tgcaaagctc ccccaagttc gtacaagcaa gcttgatgca
     2161 tggtctgaaa ggaaactgga caccttatgg cggaatgcaa attgcagaag attatcaggc
     2221 cttcaacctt ggtattggta aagatttagg actttttggt gccttttctt tcgatatcac
     2281 gcaggccaat acgacgcttg cagatgacac ccgtcacagc ggacaatcgg ttaaatccgt
     2341 ctacagcaaa tccttctacc agacgggaac caatatccag gtcgcaggat atcgctattc
     2401 tacgcaaggt ttttataact tatccgacag tgcatacagt cgaatgagtg gttacaccgt
     2461 caagcctcct accggagaca ccagtgagca gacactattt attgattatt ttaatctgtt
     2521 ctacagtaag cgtggtcagg aacaaataag catctctcag cagcttggac attacggtac
     2581 gacatttttc agtgccagtc gccaaagtta ctggaacacg tcacgcagcg accagcaaat
     2641 atcatttgga ttaaatgtgc cgcttggtga tattacgact tcgctgaatt acagctattc
     2701 caataatata tggcaaaacg atcgggatca tttactcgct tttacactta atgttccctt
     2761 tagtcattgg atgcgtacag acagtcagtc ggcatttcgt aattcaaacg ccagttacag
     2821 tatgtcaaac gatttgaaag gcggcatgac caacctatca ggggtttatg gcactctgct
     2881 gccggataat aacctgaatt atagcgttca ggtcggtaac acccaagggg gtaatacatc
     2941 atctggcacc agtggttaca gttctcttaa ttatcgtgga gcttacggta atacgaatgt
     3001 cggttacagt cggagtggtg acagcagcca gatttattac ggaatgagtg gtgggattat
     3061 tgctcatgct gatggcatca cctttggaca gccgctgggc gacacaatgg ttctggttaa
     3121 ggctcctggc gctgataatg tcaaaataga gaaccagacc ggaattcata ccgactggcg
     3181 tggctatgcc atattaccat ttgcgacaga atatagagaa aatcgtgtcg ctcttaacgc
     3241 gaattccctt gcagataatg ttgaactgga tgaaaccgta gtcactgtca tcccaactca
     3301 cggtgctatt gccagagcaa catttaatgc acaaatcggc ggaaaagtat taatgacgtt
     3361 gaagtacggt aataaaagcg ttccattcgg tgcaattgtc actcacggag agaataaaaa
     3421 tggcagcatt gtcgcggaaa atggtcaggt ttatctgact ggacttccac agtcagggaa
     3481 attacaggtt tcatggggca atgataaaaa ctcaaactgt attgtcgatt acaagcttcc
     3541 tgcagtctct cctggaacct tgctgaacca gcagacagca atctgtcgct aaggaacaat
     3601 agattatgaa atacaataac gttattttcc tcggtttatg tctggggtta accacctatt
     3661 ctgctttatc cgcagatagc gttattaaaa ttagcgggcg cgttcttgat tatggctgca
     3721 cagtctcatc ggattcgctt aattttaccg tagatctcca aaaaaacagt gccagacaat
     3781 ttccaacgac cggtagcaca agtccagccg tcccttttca gattacgtta agtgaatgca
     3841 gcaaagggac aacgggagtt cgggttgcat ttaacggtat tgaggatgca gaaaataata
     3901 ctctgttgaa actggatgaa ggaagcaata cggcctccgg tttgggtata gaaatactgg
     3961 acggaaatat gcgtccggtg aaattgaatg accttcatgc cgggatgcag tggatcccat
     4021 tggtaccaga acagaacaat attttgcctt actccgctcg tatgaagtca actcagaagt
     4081 ccgtcaatcc aggactggtg agcgcttcgg caacctttac ccttgaattt caataatcag
     4141 agtcagatat gaaaagactt cacaagaggt tcctgttagc tacgttttgc acgttattta
     4201 cagcaactct ccaggccgcc gatgtcacta tcactgttaa tggtcgggta gtcgctaaac
     4261 catgcactat tcaaaccaaa gaagctaacg ttaatctcgg ggatctttat acacgcaatc
     4321 tgcaacaacc tggttctgca tctggctggc acaatattac tttgtcatta accgattgtc
     4381 cgattgaaac aagtgcagtg acggcaatcg tgacaggttc aactgacaat acgggttatt
     4441 acaaaaatga aggtactgcc gaaaatattc agatagagct aagggatgac caggatgcga
     4501 cgttaaaaaa tggcgatagc aaaacggtta ttgttgatga gatcactcgt aatgcacagt
     4561 ttccacttaa ggcaagagct atcacggtga atggaaacgc aagccaggga acgatcgagg
     4621 c
//
LOCUS       DAHHTG010000222         4514 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.226, whole genome shotgun sequence.
ACCESSION   DAHHTG010000222 DAHHTG010000000
VERSION     DAHHTG010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4514
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.226"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            545..1261
                     /gene="nanC"
                     /locus_tag="NBI74_004420"
     CDS             545..1261
                     /gene="nanC"
                     /locus_tag="NBI74_004420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminic acid outer membrane channel
                     NanC"
                     /protein_id="HCD1934645.1"
                     /translation="MKKAKILSGVLLLCFSSPLISQAATLDVRGGYRSGSHAYETRLK
                     VSEGWQNGWWASMESNTWNTIHDNKKENAALNDVQVEVNYAIKLDDQWTVRPGMLTHF
                     SSNGTRYGPYVKLSWDATKDLNFGIRYRYDWKAYRQQDLSGDMSRDNVHRWDGYVTYH
                     INSDFTFAWQTTLYSKQNDYRYANHKKWATENAFVLQYHMTPDITPYIEYDYLDRQGV
                     YNGRDNLSENSYRIGVSFKL"
     gene            1281..2387
                     /gene="nanM"
                     /locus_tag="NBI74_004421"
     CDS             1281..2387
                     /gene="nanM"
                     /locus_tag="NBI74_004421"
                     /EC_number="5.1.3.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418730.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate epimerase"
                     /protein_id="HCD1934646.1"
                     /translation="MNKTITALAIMMASFAANASVLPETPVPFKSGTGAIDNDTVYIG
                     LGSAGTAWYKLDTQAKDKKWTALAAFPGGPRDQATSAFIDGDLYVFGGIGKNSKGLTQ
                     VFNDVHKYNPKTNSWVKLMSHAPMGMAGHVTFVHNGKAYVTGGVNQNIFNGYFEDLNE
                     AGKDSTAIDKINAHYFDKKAEDYFFNKFLLSFDPSTQQWSYAGESPWYGTAGAAVVNK
                     GDKTWLINGEAKPGLRTDAVFELDFTGDNLKWNRLAPVSSPDGVAGGFAGISNDSLIF
                     AGGAGFKGSRENYQNGKNYAHEGLKKSYSTDIHLWHNGKWDKSGELSQGRAYGVSLPW
                     NNSLLIIGGESAGGKAVTDSVLISVKDNKVTVQN"
     gene            2451..3431
                     /locus_tag="NBI74_004422"
     CDS             2451..3431
                     /locus_tag="NBI74_004422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HCD1934647.1"
                     /translation="MNAIISPNYYYVLTVAGQSNAMAYGEGLPLPDREDAPHPRIKQL
                     ARFTHTHPGGPPCHFNDIIPLTHCPHDVQDMQGYHHPLATNHQTQYGTVGQALHIARK
                     LLPFIPDNAGILIVPCCRGGSAFTAGSEGTYSERHGASHDACRWGTDTPLYQDLVSRT
                     RAALAKNPHNKFLGVCWMQGEFDLMTSDYASHPQHFNHMVEAFRRDLKQFHSQLNNIT
                     AAPWFCGDTTWYWKENFPHAYEAIYGNYQNNVLANIIFVDFQQQGERGLTNAPDEDPD
                     DLSTGYFGSAYRSAENWTTSLRSSHFSAAARRGIISDRFVEAILQFWRER"
     gene            complement(4014..>4514)
                     /locus_tag="NBI74_004423"
     CDS             complement(4014..>4514)
                     /locus_tag="NBI74_004423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000378101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase"
                     /protein_id="HCD1934648.1"
                     /translation="LISAHTQISTLHGVEQHDGFLNKTLAGAQKKITIISPWLSWQKV
                     EQTGFLASMALARSRGIDITVVTDKNCNIAHVDDDKRQEKQHLLNDAVEKLNKMGIAT
                     KLVNRVHSKIVIEDEELLCVGSFNWFSAAREDKYQRYDTSLVYRGEGVKNEIKAIYGS
                     LDQRQL"
BASE COUNT         1333 a          970 c          999 g         1212 t
ORIGIN      
        1 tcgtctggct tcacatggca tctgcctctt agaaaaagat cgacatattt tgtgacacga
       61 attgcaaatc tggttttgtt gtatggattg cgtgattttt gatctggtat aacaggtata
      121 aaggtgcacc acgatagtca atgaaactgg gcatctcgca atctatggca aacatcactt
      181 cagttctttc tcatcgggtg atgaaaacgc acttcagtct gaaaggaata tgaaaatgag
      241 atcaacagac actctatttt atgactctgg gtaaaatgga ttgagtaagc gatatagctt
      301 acgaaaattc aaatcaatta aacatcagca gagattttat actcaggtat ttaatctggc
      361 tctctgttta tttaaataat gtggaaagag atttttcaca ggagacatta tacaaaaaat
      421 ataaaataca gctacccgtt gccaaagaca ctataagcct ggcaaaaaat attacacaac
      481 ataaatgcta attgtttatg cgggctttgt attgctttct gtatcctaca aatgagtgaa
      541 atttatgaaa aaggctaaaa tactttctgg cgtattatta ctgtgctttt cgtccccatt
      601 aatttctcag gctgcgacac tggacgtacg tggtggatat cgtagtggaa gccacgccta
      661 tgagactcga ctcaaagtca gtgagggatg gcaaaatgga tggtgggcaa gcatggaaag
      721 taatacctgg aataccattc atgataataa aaaggaaaat gccgcactca atgatgttca
      781 ggttgaagtt aattacgcaa ttaaacttga tgatcaatgg acggtacgcc cgggaatgtt
      841 aacgcatttt agcagcaacg gcacacgtta cggaccctac gtaaaactgt cctgggacgc
      901 gacaaaagat cttaattttg gcattcgcta tcgttacgac tggaaagctt accgacaaca
      961 agacttatcc ggtgatatgt ctcgtgataa cgttcatcgt tgggatggat atgtcactta
     1021 ccatattaat agtgatttca ccttcgcatg gcaaacgacg ctatacagca aacagaacga
     1081 ttatcgctat gcaaaccata agaaatgggc gacggaaaat gcatttgttc tacaatacca
     1141 tatgacgccc gatattacgc catacataga atatgactac cttgaccgtc agggtgttta
     1201 caacggcaga gataatttat cggaaaacag ttatcgcatt ggtgtgtcat ttaaactgta
     1261 gtagacagga gacagtcaca atgaataaaa caataacggc gcttgctatc atgatggctt
     1321 catttgccgc aaacgcgtct gtattaccgg aaactcctgt gccatttaaa agtggtaccg
     1381 gagcaattga taacgacact gtctacattg gtttaggtag cgcaggtacg gcatggtaca
     1441 agctggatac acaggccaaa gataaaaaat ggacagcgtt agctgcattc cctggtggac
     1501 caagagatca agcaacctcg gcatttattg atggtgatct gtatgtgttt ggcggcattg
     1561 gcaaaaatag caagggcttg actcaggtat ttaatgacgt acacaaatac aaccccaaaa
     1621 ccaatagctg ggttaaattg atgtcgcacg cgccaatggg catggcgggc catgtgactt
     1681 ttgtacacaa cggcaaagct tatgttactg gcggtgttaa ccagaatatc ttcaatggct
     1741 attttgaaga tctcaacgag gctggaaaag attcaaccgc tatagataaa atcaacgctc
     1801 actattttga caaaaaagca gaagattatt tcttcaataa gtttctgttg tcttttgatc
     1861 cctcaaccca gcaatggagt tacgctggcg aatctccctg gtacggaacg gctggtgcgg
     1921 cggttgtgaa taaaggtgat aaaacctggc ttattaatgg cgaagccaaa cccggattgc
     1981 gaacggatgc cgtatttgaa cttgatttca ccggtgataa tttaaaatgg aataggcttg
     2041 ctcccgtctc atcaccagat ggcgtcgctg gcggttttgc ggggataagc aatgattctc
     2101 ttatatttgc cggaggggcc ggattcaaag gttcacgaga aaattaccag aacggtaaga
     2161 actatgcgca tgaaggcctg aaaaaatcat atagcactga tattcatctt tggcataacg
     2221 ggaaatggga taaatcgggt gaattatcgc aaggtcgggc ctacggagta tcattgccct
     2281 ggaataatag tctattgatt attggcggtg aatctgcagg cggcaaagcg gtgacggatt
     2341 cagttttgat ctctgtgaag gataataaag tcacagtaca aaactaacgc ttcagggccc
     2401 cggtaaaggg ccctgctatc aatttgtcat ttcaattacg cgaaaattat atgaacgcaa
     2461 taatatcgcc caattattac tatgttctta ccgttgctgg tcagtctaat gccatggcgt
     2521 atggcgaagg actgccatta ccggacaggg aagatgcgcc tcatcccaga atcaaacaat
     2581 tagcgagatt tacgcatacg catcccggag gcccgccatg tcactttaac gacattattc
     2641 cactgactca ctgcccacac gatgttcagg atatgcaggg ttatcaccat cctctggcaa
     2701 cgaatcatca aacacagtac ggcaccgttg gccaggcact gcatattgca cggaaattac
     2761 tgcccttcat tcctgataat gcagggattc tcatcgtccc gtgttgccgt ggcggatcgg
     2821 cttttaccgc gggcagcgag gggacatatt cagaacggca cggagccagc catgatgctt
     2881 gtcgttgggg aacggatacc ccgctctacc aggatttagt cagcagaacg cgagccgcac
     2941 tggcaaaaaa tccgcataac aaattcctcg gcgtatgctg gatgcaaggc gaatttgact
     3001 taatgaccag tgactacgcg tcacaccctc aacactttaa tcatatggtt gaagcctttc
     3061 gtagggatct aaaacaattc cattctcagc ttaataatat tactgccgcg ccgtggtttt
     3121 gcggcgatac cacctggtac tggaaagaaa atttccctca tgcgtatgaa gctatttatg
     3181 gcaattatca aaataatgtt ttagccaata ttattttcgt cgacttccag caacaaggtg
     3241 aaagaggact gacgaacgcg cctgatgaag atccggacga tttaagcacg ggatatttcg
     3301 gttcagcgta ccggtcagcg gagaactgga cgacgtcact gcgaagcagt catttcagcg
     3361 cggcagcccg tcgggggatt atttctgaca gatttgtaga agcaattttg cagttttggc
     3421 gcgaaaggtg agcggggagt ttattcatgg gggatgtcct tgtccattcc cagagcagac
     3481 aacaaaaaac aaatgagatc aaggtaatat ctcttcactt gcgggctcga acagcaggca
     3541 attgccaata taataatctg gcacaagggt gaaaaacgcg caccgcataa accgttgtta
     3601 ttgcttacag attagccgga tgccttaata gacatccgcg ccttttcggt tatggctcgg
     3661 aaatctacga acatctacac agtttactgg aacgttttgg gccacagcgt tcgcagcacc
     3721 gtcctgatat gccgttctgg cgattataag tcgacggatt ctggcaaata gacgatgatg
     3781 atatgcttaa tgatgttcaa agaaattagg ccattattat attattgctt gcattttctt
     3841 tcattcctaa aagcacccgc cattaatccc aggcgcatta tgcaaaggaa aatgattagg
     3901 taaacaataa gatttataat caggaaaggc gactcgcaat aaaacggaaa gtcgccttaa
     3961 cattggtaaa atatgccctt tactactcct gtatcatgaa cggctcatca catttaaagc
     4021 tgacgttgat ccagactacc atatattgcc tttatttcat tttttacgcc ttcgccacgg
     4081 tacaccaaag acgtatcgta tcgttgatac ttatcttccc gtgcagcact gaaccagtta
     4141 aaagatccga cacacagcag ttcttcatcc tcaatcacaa ttttgctgtg gacacgatta
     4201 accaatttcg tagcgatacc catcttattg agcttttcaa cagcatcatt cagcagatgt
     4261 tgtttctcct gacgcttatc atcatcgaca tgcgctatat tgcagttttt atccgtcacc
     4321 acggtaatat caatgccccg ggaacgggcc aaagccattg atgccagaaa tcccgtttgc
     4381 tccacttttt gccaagataa ccaaggggaa ataatcgtta ttttcttttg agctccggca
     4441 agagtcttgt tcaaaaatcc atcatgctgc tccacaccat gcagcgttga aatttgtgta
     4501 tgtgcggaaa ttaa
//
LOCUS       DAHHTG010000223         4432 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.227, whole genome shotgun sequence.
ACCESSION   DAHHTG010000223 DAHHTG010000000
VERSION     DAHHTG010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4432)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4432)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4432
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.227"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            176..1084
                     /locus_tag="NBI74_004424"
     CDS             176..1084
                     /locus_tag="NBI74_004424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prohibitin family protein"
                     /protein_id="HCD1934649.1"
                     /translation="MKAPVSITSFRPQKSIAIAIGVLAVVVLPFLSYYTVNEGERGIL
                     LRYGKIVKVAEPGLGFKIPFMESVEKISTRNQAVVYQGLQAYSRDQQPAQMTVSVSFH
                     IKPSEAGAVYTTYNTIEALKDRLIVRQLPTQLENVFGQYTAISAVQDRTKLVQDLQNA
                     MRKAVVGPVVIDGVQIENIDFSDAYEKSIEDRMKAEVAIATRKQNLETEKIQAQIAVT
                     QAQAEADSKLAAAKAEAETIRVRGAAEAETIRLKSAAEAEAIRLRGEALRENPGLVAL
                     TTAERWDGKLPDTMIPGSTVPFISTK"
     gene            complement(1292..3283)
                     /gene="tkt"
                     /locus_tag="NBI74_004425"
     CDS             complement(1292..3283)
                     /gene="tkt"
                     /locus_tag="NBI74_004425"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708699.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HCD1934650.1"
                     /translation="MSSRKELANAIRALSMDAVQKAKSGHPGAPMGMADIAEVLWRDF
                     LKHNPQNPSWADRDRFVLSNGHGSMLIYSLLHLTGYDLPMEELKNFRQLHSKTPGHPE
                     VGYTAGVETTTGPLGQGIANAVGMAIAEKTLAAQFNRPGHDIVDHYTYAFMGDGCMME
                     GISHEVCSLAGTLKLGKLIAFYDDNGISIDGHVEGWFTDDTAMRFEAYGWHVIRDIDG
                     HDAASIKRAVEEARAVTDKPSLLMCKTIIGFGSPNKAGTHDSHGAPLGDAEIALTREQ
                     LGWKYAPFEIPSEIYAQWDAKEAGQAKESAWNEKFAAYAKAYPQEAAEFTRRMKGEMP
                     SDFDAKAKEFIAKLQANPAKIASRKASQNAIEAFGPLLPEFLGGSADLAPSNLTLWSG
                     SKAINEDAAGNYIHYGVREFGMTAIANGISLHGGFLPYTSTFLMFVEYARNAVRMAAL
                     MKQRQVMVYTHDSIGLGEDGPTHQPVEQVASLRVTPNMSTWRPCDQVESAVAWKYGVE
                     RQDGPTALILSRQNLAQQERTEEQLANIARGGYVLKDCAGQPELIFIATGSEVELAVA
                     AYEKLTAEGVKARVVSMPSTDAFDKQDAAYRESVLPKAVTARVAVEAGIADYWYKYVG
                     LNGAIVGMTTFGESAPAEQLFEEFGFTVDNVVAKAKALL"
     gene            3561..4319
                     /gene="loiP"
                     /locus_tag="NBI74_004426"
                     /gene_synonym="yggG"
     CDS             3561..4319
                     /gene="loiP"
                     /locus_tag="NBI74_004426"
                     /gene_synonym="yggG"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease LoiP"
                     /protein_id="HCD1934651.1"
                     /translation="MKIRALLVAMSVATVLTGCQNMDSNGLLSSGAEAFQAYSLSDAQ
                     VKTLSDQACQDMDSKATIAPANSEYAKRLTTISRALGDNINGQPVNYKVYMAKDVNAF
                     AMANGCIRVYSGLMDMMTDNEVEAVLGHEMGHVALGHVKKGMQVALGTNAIRVAAASA
                     GGIVGSLSQSQLGDLGEKLVNSQFSQRQEAEADDYSYDLLRQRGISPAGLATSFEKLA
                     KLEEGRQSSMFDDHPASAERAQHIRDRMSADGVK"
BASE COUNT         1067 a         1157 c         1168 g         1040 t
ORIGIN      
        1 tgttattgta tttgtgatgc atatctctat ttatttattg aatgtccgaa agcatacttt
       61 tctaatgcaa tgttttttcg ctatacccct ttatagattc tgaattctca cctgccagct
      121 gttttctcct ccccccatgt gacatattcc catactttac tcaaggaaga ttcatatgaa
      181 agcacctgtt tcaataacct catttcgccc acaaaaatct attgctatcg ccattggcgt
      241 gcttgccgtg gttgtgctgc catttctctc gtattacacc gttaatgaag gtgaacgcgg
      301 cattttgctg cgctacggaa aaatcgtaaa agttgccgag ccggggctgg gttttaagat
      361 cccttttatg gaaagtgtag agaaaatttc cacgcgtaat caggctgtgg tctatcaggg
      421 attacaggcc tacagccgcg accagcaacc ggcacaaatg acggtatcag tcagcttcca
      481 cattaaacct tcagaagcag gcgcggttta taccacctac aacaccattg aagcattaaa
      541 agatcgtctg attgtccgtc agttgccaac tcaactggag aacgttttcg gccaatatac
      601 cgccatcagc gcggtacagg atcgtacaaa actggttcag gatctgcaaa atgcaatgcg
      661 taaagccgtt gttggcccgg tggtgattga tggtgtccag attgaaaata ttgatttttc
      721 tgacgcttat gaaaagtcga ttgaagaccg aatgaaagca gaagtcgcca ttgccacacg
      781 taagcaaaat ctggaaacag agaaaatcca agcgcaaata gcagtaactc aggcccaggc
      841 agaagcagac agtaaactgg cggcagccaa agcagaagct gagaccattc gcgtaagagg
      901 cgcggcagag gctgaaacta ttcgtttaaa aagtgcggct gaagcagaag ctatccgcct
      961 gcgcggtgaa gctctgcggg agaatcccgg acttgttgcc ctcaccactg ctgagcgttg
     1021 ggacggtaaa ctgcccgata ccatgatccc cggcagcaca gttccgttta tctctactaa
     1081 gtaaaaaaag ggccgctttc gcggcccttg agactgatag caaagacaaa actgcctgat
     1141 gcgctacgct tatcaggctg caatatgttg aatttgcaaa atcttgtagg acggataagg
     1201 cgttcacgcc gcatccggca taaacaacgc gcactttgcc aacaatctga agggccgcat
     1261 ttgcggccct tctcacaaag catcttaccg attacagcag cgcttttgct ttcgcaacaa
     1321 cgttgtcaac ggtgaagccg aactcttcaa acagctgctc tgccggagca gattcaccga
     1381 aggtggtcat gccgacgata gcaccgttca ggccaacata cttgtaccag tagtcagcaa
     1441 tgccagcttc caccgcaaca cgagcggtaa ccgctttcgg cagtacggat tcacggtaag
     1501 ccgcgtcctg cttgtcgaat gcgtcggtag acggcatgga aaccacgcgc gctttcacgc
     1561 cttcggcagt caatttttcg tatgccgcta cagccagttc aacttcagaa ccggtcgcga
     1621 tgaagatcag ttccggctga ccagcacagt ctttcagcac ataaccaccg cgcgcgatgt
     1681 ttgccagctg ctcttcagta cgttcctgct gcgccaggtt ctgacgggag aggataagcg
     1741 cagtcgggcc gtcctgacgt tcaacgccgt atttccacgc taccgcggat tcaacctggt
     1801 cacacggacg ccatgtagac atgttcgggg tcacgcgcag agaagcgacc tgctctaccg
     1861 gctggtgagt cgggccatct tcgcccagac cgatggagtc gtgggtgtaa accatcacct
     1921 gacgctgttt catcagcgca gccatacgta cggcgttacg tgcgtattcc acgaacatca
     1981 ggaaggtgga ggtgtacggc aggaaaccac cgtgcaggga gataccgttg gcaatcgcag
     2041 tcataccgaa ttcacgtaca ccgtagtgga tgtagttacc cgcagcatct tcgttgattg
     2101 ctttagaacc agaccacagg gtcaggttag acggcgccag gtctgcagaa ccgccgagga
     2161 attccggcaa cagcggcccg aacgcttcga tagcattctg agacgcttta cggctggcga
     2221 tcttcgccgg gttagcctgc agtttagcaa tgaactcttt cgctttcgcg tcgaagtcag
     2281 acggcatttc gcctttcata cggcgggtaa attcagcggc ttcctgcgga taagctttcg
     2341 cgtaagcagc gaatttctcg ttccatgcgg actctttcgc ctggcctgct tctttcgcat
     2401 cccactgagc atagatttca gacgggattt cgaacggcgc gtatttccag cccagctgtt
     2461 cgcgggtcag ggcaatttca gcgtcgccca gcggcgcacc gtgggagtcg tgggtaccgg
     2521 ctttgttcgg ggaaccgaaa ccgatgatgg ttttgcacat cagcagggac ggtttgtcag
     2581 tcactgcgcg cgcttcttct actgcgcgtt tgatggatgc cgcgtcatga ccgtcgatgt
     2641 cgcgaataac gtgccagccg taagcttcga aacgcattgc ggtgtcgtcg gtaaaccagc
     2701 cttcaacgtg accatcgata gaaataccgt tgtcatcgta gaacgcaatc agtttaccca
     2761 gcttcagcgt acccgccaga gagcaaactt cgtgggagat gccttccatc atgcagccgt
     2821 cgcccatgaa ggcgtaggtg tagtggtcga cgatgtcgtg gcccggacgg ttaaactgcg
     2881 ccgccagcgt tttttctgca atcgccatac cgactgcgtt ggcaataccc tgacccagcg
     2941 gaccggtggt ggtttctaca cctgcggtgt aacccacttc cgggtggccc ggagttttag
     3001 agtgcagctg acggaagttt ttcagttctt ccatcggcag atcgtaaccg gtgaggtgca
     3061 gcaggctgta gatcagcatg gagccgtggc cgttggacag cacgaagcgg tcacggtcag
     3121 cccaggacgg attctgcggg ttgtgtttca ggaaatcacg ccacaggact tcggcaatgt
     3181 cagccatacc cataggggca cccgggtgac cggatttggc tttctgtact gcgtccatgc
     3241 tcagcgcacg aatagcattg gcaagctctt tacgtgagga cattttgact ccagatcgga
     3301 tgatgaaggg cacgccctta acgacttgac gacagcgcgt tttgggctac gccggaaaat
     3361 ttgccaacaa tttaccgcaa gccgcgcgtc atgtacatgg aacatccttt tgccgcttca
     3421 gaaatctctg gatcatgctc gcatgttgcg caatctactc gccggtccac tgcacttttc
     3481 cttatacttc gattgagcgt caattcacct gcagatggcg catttttaga ataatcctga
     3541 ccttgtgcgg aagagaaaac atgaaaattc gcgccttatt ggtagcaatg agcgtggcaa
     3601 cggtactgac tggttgccag aatatggact ccaacggact gctctcatca ggagcggaag
     3661 cttttcaggc ttacagtttg agtgatgcgc aggtgaaaac cctgagcgat caggcatgtc
     3721 aggatatgga cagcaaggcg acgattgcgc cagccaatag cgaatacgct aaacgtctga
     3781 cgacaatttc tcgcgcgtta ggcgataaca tcaatggtca gccggtgaat tacaaagtgt
     3841 atatggcgaa ggatgtgaac gcctttgcaa tggctaacgg ctgtatccgc gtctatagcg
     3901 ggctgatgga tatgatgacg gataacgaag tcgaagcggt gctcggtcac gaaatggggc
     3961 acgtggcatt aggccatgtg aagaaaggaa tgcaggtagc acttggtaca aacgcaattc
     4021 gcgttgcggc ggcctctgca ggcggaattg tcggcagttt gtcacaatca caactaggag
     4081 acctgggcga aaagctagtc aattcgcaat tctcccagcg ccaggaagca gaggccgatg
     4141 attactccta cgatttgctg cgccaacgcg gcatcagtcc ggctggtctt gccaccagct
     4201 ttgaaaaact ggctaaactg gaagaaggtc gccaaagttc aatgtttgac gaccacccag
     4261 catccgccga acgcgcccaa catattcgcg atcgtatgag cgcggatgga gttaagtaag
     4321 gcatggtggt gccaaacgta ggtcagataa ggcgtttacg ccgcatctga catgaatggc
     4381 acatttgtta tcctgtgcgc gttgccggat gcgatgctgg cgcatcttat cc
//
LOCUS       DAHHTG010000224         4401 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.228, whole genome shotgun sequence.
ACCESSION   DAHHTG010000224 DAHHTG010000000
VERSION     DAHHTG010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4401)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4401)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4401
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.228"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            230..484
                     /gene="bssS"
                     /locus_tag="NBI74_004427"
     CDS             230..484
                     /gene="bssS"
                     /locus_tag="NBI74_004427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415578.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssS"
                     /protein_id="HCD1934652.1"
                     /translation="MEKNNEVIQTHPLVGWDISTVDSYDALMLRLHYQTPNKSEQEGT
                     EVGQTLWLTTDVARQFISILEAGIAKIESGDFQVNEYRRH"
     gene            599..1717
                     /gene="solA"
                     /locus_tag="NBI74_004428"
     CDS             599..1717
                     /gene="solA"
                     /locus_tag="NBI74_004428"
                     /EC_number="1.5.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-methyl-L-tryptophan oxidase"
                     /protein_id="HCD1934653.1"
                     /translation="MKYDLIIIGSGSVGAAAGYYATRAGLNVLMTDAHMPPHQHGSHH
                     GDTRLIRHAYGEGEKYVPLVLHAQMLWDELSRHNEDDPIFVRSGVINLGPADSAFLAN
                     VAHSADQWQLNVEKLDAQGIMARWPEIRVPDNYIGLFETDSGFLRSELAIKTWIQLAK
                     EAGCAQLFNCPVTAIRHDDDGVTIETADGEYQAKKAIVCAGTWVKDLLPELPVQPVRK
                     VFAWYQADGRYSVKNKFPAFTGELPNGDQYYGFPAENDALKIGKHNGGQVIHSADERV
                     PFAEVASDGSEAFPFLRNVLPGIGCCLYGAACTYDNSPDEDFIIDTLPGHDNTLLITG
                     LSGHGFKFASVLGEIAADFAQDKKSDFDLTPFRLSRFQ"
     gene            1765..1878
                     /locus_tag="NBI74_004429"
     CDS             1765..1878
                     /locus_tag="NBI74_004429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceO family protein"
                     /protein_id="HCD1934654.1"
                     /translation="MRRLLHYLINNIREHLMLYLFLWGLLAIMDLIYVFYF"
     gene            2139..2705
                     /locus_tag="NBI74_004430"
     CDS             2139..2705
                     /locus_tag="NBI74_004430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HCD1934655.1"
                     /translation="MSFTNTPERYGVISAALHWLSAIIVYGMFALGLWMVTLSYYDGW
                     YHKAPELHKSIGILLMMGLVIRVLWRVISPPPGPLPSYSPMTRLAAKAGHLALYLLLF
                     AIGISGYLISTADGKPISVFGWFDVPATLSDAGAQADFAGALHFWLAWSVVVLSVMHG
                     FMALKHHFIDKDDTLKRMLGKSSSDYGV"
     gene            2709..3284
                     /locus_tag="NBI74_004431"
     CDS             2709..3284
                     /locus_tag="NBI74_004431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HCD1934656.1"
                     /translation="MKKSLLGLTFASLMFSAGSAVAADYKIDKEGQHAFVNFRIQHLG
                     YSWLYGTFKDFDGTFTFDEKNPAADKVNVTINTTSVDTNHAERDKHLRSADFLNTAKY
                     PQATFTSTSVKKDGDELDITGNLTLNGVTKPVTLEAKLIGQGDDPWGGKRAGFEAEGK
                     IKLKDFNIKTDLGPASQEVDLIISVEGVQQK"
     gene            complement(3326..4378)
                     /locus_tag="NBI74_004432"
     CDS             complement(3326..4378)
                     /locus_tag="NBI74_004432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706977.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HCD1934657.1"
                     /translation="MPVLHNRISNDALKAKMLAESEPRTTISFYKYFHIADPKVTRDA
                     LYQLFTALNVFGRVYLAHEGINAQISVPASNVETFRAQLYAFDPALEGLRLNIALDDD
                     GKSFWVLRMKVRDRIVADGIDDPHFDASNVGEYLQAAEVNAMLDDPDALFIDMRNHYE
                     YEVGHFENALEIPADTFREQLPKAVEMMQAHKDKKIVMYCTGGIRCEKASAWMKHNGF
                     NKVWHIEGGIIEYARKAREQGLPVRFIGKNFVFDERMGERISDEIIAHCHQCGAPCDS
                     HTNCKNDGCHLLFIQCPVCAEKYKGCCSEICCEESALPPEEQRRRRAGRENGNKIFNK
                     SRGRLNTTLGIPDPTE"
BASE COUNT         1140 a         1070 c         1003 g         1188 t
ORIGIN      
        1 gtgagtacaa aatttctaca cttactcaaa ataattgcac aatatgctaa aaaaatatga
       61 atgacatgct gttttattgt tcatataaat cttaaaagca gaaatttatc tgttccccct
      121 ttaaaaattc ggtgaatacc cttacttatt ggtatactga agttgcttca gataaaaacg
      181 cattgaacct cgaataacgt tgtctagtaa cacgaattag ggggccatga tggaaaaaaa
      241 taatgaagtc attcagactc atccgctcgt agggtgggac atcagcaccg ttgatagcta
      301 tgatgcgctg atgttgcgtt tgcactacca gaccccaaat aagtccgagc aggaagggac
      361 tgaagttggt cagacgctct ggttaaccac tgatgttgcc agacaattta tttcgatatt
      421 agaagcagga atcgccaaaa ttgaatccgg tgatttccag gtaaacgagt atcggcgtca
      481 ttaacatgac atgaccatcc acccaaaagg cacctcaccg gtgcctttac catttctggg
      541 ttgtatatct gcattcagtt aattactctg cttaaaccat gagagcagag agaaaaaaat
      601 gaaatacgat ctcatcatta ttggcagcgg ttccgtaggc gctgccgccg ggtattatgc
      661 aacccgcgca ggtttaaacg tgctaatgac cgacgcccat atgccaccgc atcaacacgg
      721 cagccaccac ggcgatacgc gattaattcg ccatgcttat ggtgaaggcg aaaagtatgt
      781 cccgctggtc ctccacgcgc aaatgctgtg ggatgaactt tcccgccaca acgaagatga
      841 tcccattttt gtacgctctg gtgtcattaa cctcggtccg gctgactccg catttctcgc
      901 caacgtcgcc cacagcgccg accagtggca actcaacgtt gaaaagctcg acgcgcaagg
      961 gattatggcc cgctggccag aaatacgcgt cccggacaac tacatcggtt tatttgaaac
     1021 tgattccggt tttttgcgca gcgaactggc gattaaaacc tggatccaac tggcgaagga
     1081 agcgggctgt gcgcagctgt tcaactgccc ggtcaccgca attcgtcatg acgatgatgg
     1141 cgtaactatt gaaacggcag acggtgagta tcaggcgaaa aaagcgattg tctgcgcggg
     1201 aacatgggta aaagacctac tcccggagct gcctgtccag cccgtacgca aagtatttgc
     1261 ctggtatcag gccgatggcc gctatagcgt gaagaataaa ttcccggcat ttaccggtga
     1321 gctgcccaat ggcgatcaat attatggttt tccggcagaa aacgacgcgt tgaagattgg
     1381 caaacataac ggaggccagg ttatccattc agcggatgaa cgtgttccgt ttgcggaagt
     1441 ggccagcgat ggttcggaag ccttcccgtt cttgcgcaat gtgttgccgg gtatcggttg
     1501 ctgcctgtac ggcgctgcct gcacctatga taattcgcct gacgaagatt ttattatcga
     1561 taccctaccc ggccacgata atacactgct cattaccggc ctgagtgggc acggttttaa
     1621 atttgcgtca gttttagggg aaatagctgc cgattttgcg caagacaaaa aaagcgattt
     1681 tgatttgacg ccattcaggc tttcccgctt ccaataatca tcattacggc ctccagaatg
     1741 aggccgtttt tacaggagta tttaatgcgt cgactgttgc actatctcat caataatatt
     1801 cgcgagcatc tgatgcttta tctttttctc tggggattgt tggcaattat ggatctgatc
     1861 tatgtgtttt atttttagtt ttccgcactg acataaactg acaaatattt aatctccctt
     1921 tcatttagcg tgaaaaattg aatgagattc ttcctgcctc tacagcactc actagacctt
     1981 ccagatgctt aacccactaa acttatgaag attctttcat tattaattca aaattattga
     2041 aataaaaata aatatcattc aatttgttat ttttgtgttg caattcattc tccgacacgc
     2101 cattttcttt ctctcgcaaa ttcgttttgg atgtacttat gtcattcaca aatacccctg
     2161 aacgctatgg tgttatatct gcggccttac attggctaag cgcaattatt gtctacggca
     2221 tgtttgcttt aggtctgtgg atggtcacgc tcagttatta cgatggctgg tatcacaaag
     2281 cacccgaact gcataaaagc atcggtattt tgttaatgat ggggctggtt attcgcgttc
     2341 tgtggcgtgt catatctcca ccgccgggtc cgctgccgag ttattcgccc atgactcgtc
     2401 ttgcagcaaa agctggacat ctcgcattgt acctattgct gttcgccatc ggcatcagcg
     2461 gctatctgat ctcaactgcc gatggtaaac cgatcagcgt ttttggctgg tttgacgtcc
     2521 ccgcgaccct ctccgacgct ggcgcacagg ctgactttgc cggtgccctg catttttggc
     2581 tcgcgtggag cgttgtagta ctgtccgtta tgcacggatt tatggccctg aagcatcatt
     2641 tcatcgataa agacgacact ctgaagcgca tgctgggaaa gtcgtcatct gactatggag
     2701 tataaaaaat gaaaaaaagc ctgcttggtt taaccttcgc ttccttgatg ttctctgccg
     2761 gttcagcggt tgccgccgat tacaaaattg acaaagaagg tcagcacgcc tttgttaact
     2821 tccgaatcca gcatcttggc tatagctggt tatatggcac cttcaaagat ttcgacggta
     2881 cttttacctt tgatgaaaaa aatccagctg ccgataaagt gaatgtgaca attaacacca
     2941 ccagcgtcga tactaatcac gccgaacgcg ataaacatct tcgcagtgcg gatttcctca
     3001 ataccgcaaa atatccacag gcaacattca cctccaccag cgtgaagaaa gacggtgacg
     3061 aactggatat taccggcaat ctgacgctga atggtgtaac caagcccgtc acactggaag
     3121 cgaaattaat tggtcagggt gacgacccgt ggggcggtaa acgtgcaggc tttgaggccg
     3181 aaggcaaaat taagctcaaa gacttcaata tcaagacaga tttaggtcca gcgtctcagg
     3241 aagtggatct gattatttca gtggaaggcg tacagcagaa gtaagagtga aatgttgccg
     3301 gatggcacgc atccggcaat gatatttatt ctgttggatc aggaatgccc agtgttgtat
     3361 tcagacgtcc acgagactta ttaaagatct tattgccatt ttcacgtcct gcccgacggc
     3421 gtcgctgttc ctctggcggt aacgcgcttt cttcgcagca aatctcacta caacaacctt
     3481 tgtatttttc cgcacatact ggacactgaa taaacagcag atggcagcca tcatttttac
     3541 agttggtatg gctgtcgcac ggcgcaccgc actggtggca gtgcgcgata atctcatcag
     3601 atatacgttc gcccatccgc tcgtcaaaaa caaaattttt gccaataaaa cgcaccggca
     3661 agccctgctc gcgcgcctta cgggcgtatt caataattcc gccctcgata tgccagactt
     3721 tgttgaatcc gttatgtttc atccaggcac tggccttttc acaacgaatg ccgccggtgc
     3781 agtacatgac gattttttta tctttatgtg cctgcatcat ctcaactgct tttggcagct
     3841 gctcacggaa ggtatctgcc ggtatttcca gcgcgttttc aaagtgcccc acttcatact
     3901 catagtggtt acgcatgtcg ataaacaatg catcgggatc gtcaagcatg gcgttcactt
     3961 ccgccgcttg cagatactca ccaacattgc tggcatcaaa gtgaggatcg tcaataccgt
     4021 cggcaacaat gcgatcgcgt accttcatac gcagtaccca gaaggatttc ccgtcatcat
     4081 ccaacgcgat attcaggcgt aaaccctcta aagccggatc gaaggcatag agctgtgcgc
     4141 gaaatgtttc aacattgctc gcaggtacgc tgatttgcgc gttaatgccc tcatgcgcca
     4201 gatacactcg cccaaaaaca ttcagcgcgg taaacagttg atataaagcg tcacgggtca
     4261 ccttaggatc ggcaatgtgg aaatacttat aaaacgaaat ggtggttcgc ggttcgctct
     4321 cagccaacat tttggctttt aacgcgtcgt tggaaatgcg gttgtgtaac actggcatgg
     4381 tgtacggttc ctgcgagatg g
//
LOCUS       DAHHTG010000225         4396 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.229, whole genome shotgun sequence.
ACCESSION   DAHHTG010000225 DAHHTG010000000
VERSION     DAHHTG010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4396)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4396)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4396
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.229"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            1..430
                     /locus_tag="NBI74_004433"
                     /pseudo
     CDS             1..430
                     /locus_tag="NBI74_004433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308694.2"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            671..1636
                     /gene="lipA"
                     /locus_tag="NBI74_004434"
     CDS             671..1636
                     /gene="lipA"
                     /locus_tag="NBI74_004434"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HCD1934658.1"
                     /translation="MSKPIVMERGVKYRDADKMALIPVKNVATEREALLRKPEWMKIK
                     LPADSTRIQGIKAAMRKNGLHSVCEEASCPNLAECFNHGTATFMILGAICTRRCPFCD
                     VAHGRPVAPDANEPVKLAQTIADMALRYVVITSVDRDDLRDGGAQHFADCITAIREKS
                     PQIKIETLVPDFRGRMDRALDILTATPPDVFNHNLENVPRIYRQVRPGADYNWSLKLL
                     ERFKEAHPEIPTKSGLMVGLGETNEEIIEVMRDLRRHGVTMLTLGQYLQPSRHHLPVQ
                     RYVSPDEFDEMKAEALAMGFTHAACGPFVRSSYHADLQAKGMEVK"
     gene            complement(1736..1939)
                     /gene="tatE"
                     /locus_tag="NBI74_004435"
     CDS             complement(1736..1939)
                     /gene="tatE"
                     /locus_tag="NBI74_004435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatE"
                     /protein_id="HCD1934659.1"
                     /translation="MGEISITKLLVVAALVVLLFGTKKLRTLGGDLGAAIKGFKKAMN
                     DDDAAAKKGADVDLQAEKLSHKE"
     gene            complement(2068..2856)
                     /locus_tag="NBI74_004436"
     CDS             complement(2068..2856)
                     /locus_tag="NBI74_004436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deaminated glutathione amidase"
                     /protein_id="HCD1934660.1"
                     /translation="MLVAAGQFAVTSVWEKNVEICASLMAQAAENEVSLFVLPEALLA
                     RDDHDADLSVKSAQLLEGEFLGRLRRESKRNMMTTILTIHVPSTPGRAWNMLVALQAG
                     NIVARYAKLHLYDAFAIQESRHVDAGNEIAPLLEVEGMKVGLMTCYDLRFPELALAQA
                     LQGAEILVLPAAWVRGPLKEHHWSTLLAARALDTTCYMVAAGECGNKNIGQSRIIDPF
                     GVTIAAASEMPALIMAEVTPERVRQVRAQLPVLNNRRFAPPQLL"
     gene            2949..3332
                     /gene="crcB"
                     /locus_tag="NBI74_004437"
     CDS             2949..3332
                     /gene="crcB"
                     /locus_tag="NBI74_004437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HCD1934661.1"
                     /translation="MLQLLLAVFIGGGTGSVARWLLSMRFNPLHQAIPLGTLAANLIG
                     AFIIGMGFAWFSRMTNIDPVWKVLITTGFCGGLTTFSTFSAEVVFLLQEGRFGWALLN
                     VFVNLLGSFAMTALAFWLFSASTAH"
     gene            complement(3386..3595)
                     /gene="cspE"
                     /locus_tag="NBI74_004438"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             complement(3386..3595)
                     /gene="cspE"
                     /locus_tag="NBI74_004438"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HCD1934662.1"
                     /translation="MSKIKGNVKWFNESKGFGFITPEDGSKDVFVHFSAIQTNGFKTL
                     AEGQRVEFEITNGAKGPSAANVIAL"
     gene            complement(3770..4330)
                     /gene="pagP"
                     /locus_tag="NBI74_004439"
     CDS             complement(3770..4330)
                     /gene="pagP"
                     /locus_tag="NBI74_004439"
                     /EC_number="2.3.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) palmitoyltransferase PagP"
                     /protein_id="HCD1934663.1"
                     /translation="MNVSKYVAIFFFVFIQLISVGKVFANADEWMTTFRENIAQTWQQ
                     PEHYDLYIPAITWHARFAYDKEKTDRYNERPWGGGFGQSRWDEKGNWHGLYAMAFKDS
                     WNKWEPIAGYGWESTWRPLADENFHLGLGFTAGVTARDNWNYIPLPVLLPLASVGYGP
                     ATFQMTYIPGTYNNGNVYFAWMRFQF"
BASE COUNT         1155 a         1131 c          979 g         1131 t
ORIGIN      
        1 ccttctgact attttgaagt tgcatatgtc cataaaggaa tcgttaatgc agcgaaagtg
       61 cttaatctgc cccccctcgg caatctatca gcctattcag aaactgcgcg ttatattccc
      121 tgacctattg tttattcgca aaggccaggg tgtcactcct accgcatttg cgatgtatct
      181 atatgagtct atcactcagg gacttgagtc cattcttggc gcgctggata tcgaaggaag
      241 ctatgataag caacggacga taactattgc gccctccccc tcggtcggag ccctggtcct
      301 tcctgtcatc tatcgggcga ttaaaactca ctatccacag cttttactgc gcaacccgcc
      361 catcagcggc gcggaaaacc aactcagtca gtttcaaacc gatctcatca tcaataacat
      421 gttttgcacg gggtcataga tgttatccgt aatgcatttt gaaaaagtat tgttacagcc
      481 agatcacagc gcacaacgat aaaatggcac aatccaacaa caattttaca ttttcgcgac
      541 cgctttggct gctttcaggt ccgtttcaat gatatactgc cagtcgttaa ttcaaaaata
      601 gttgataatt acaacaatct attgaattga aacgctttcc ttcgtaattc gcaactggaa
      661 cacgcacgct atgagtaaac ccattgtgat ggaacgcggt gttaaatacc gcgatgccga
      721 taagatggcc cttatcccgg ttaaaaacgt ggcaacagag cgcgaagccc tgctgcgcaa
      781 gccggaatgg atgaaaatca agcttccagc ggactctaca cgtatccagg gcatcaaagc
      841 cgcaatgcgc aaaaatggcc tgcattctgt ctgcgaggaa gcctcctgcc ctaacctggc
      901 ggaatgcttt aaccacggca ctgcaacgtt tatgatcctc ggcgctattt gtacccgccg
      961 ttgcccgttc tgtgacgttg cccacggtcg cccggtagct cctgatgcca atgaaccagt
     1021 gaaactggcg cagaccattg ccgatatggc gctgcgttat gtggttatca cctccgttga
     1081 ccgtgatgat ctgcgcgatg gcggtgccca gcactttgcg gattgcatta ctgccattcg
     1141 ggaaaaaagc ccgcaaatca aaattgaaac tctggtgccg gatttccgcg gtcgtatgga
     1201 tcgtgctctg gatattctga ctgcaacgcc accagatgtg ttcaaccata acctggaaaa
     1261 cgtaccgcgt atttaccgtc aggtacggcc tggtgcagat tacaactggt cgcttaagct
     1321 gctggaacgc tttaaagaag cgcatccgga aatcccgacc aagtctggtc tgatggtggg
     1381 actgggtgaa accaatgaag aaattattga ggtaatgcgc gacctgcgcc gtcatggtgt
     1441 gacgatgcta acgctggggc aatatttgca gccaagccgc catcacctgc cggttcaacg
     1501 ttacgttagc ccggatgagt tcgacgaaat gaaagccgaa gcgctggcga tgggctttac
     1561 ccatgctgca tgcggtccgt ttgtccgctc ttcttaccac gccgatttgc aggcgaaagg
     1621 gatggaagtt aagtaatcct gtaataatta cttacatctg tctgataaaa aacccgcttt
     1681 ttgaagcggg tttttgtatc gaacaagata ttgagggagc gtcctgctcg ccacgtcact
     1741 ctttatgaga gagcttttca gcctgaagat caacgtctgc gccttttttc gccgcagcat
     1801 cgtcatcatt catcgccttc ttgaaccctt taatggccgc tccaaggtct ccgcccagcg
     1861 tacgtaactt cttagtccca aacagcagaa cgaccagcgc cgcaactacc agcagtttgg
     1921 taatactaat ctcacccata gataccttct tgacatataa ccggcaacag aacgcgcctt
     1981 aaaaaccaga gaatattaac ggtcattcga cgcgcgcaca caatagcaat ctgtaacaag
     2041 gtgaatcaag cgccgagtaa aaaaacatca taataattgc ggcggcgcaa agcgacggtt
     2101 gtttaagacg ggcagttgcg cgcgcacctg acgcacacgt tcgggcgtca cttccgccat
     2161 aatgagtgca ggcatttctg acgctgccgc aatggtgacg ccaaagggat ctataatccg
     2221 gctttgcccg atatttttgt tcccgcactc ccccgccgcc accatataac aggtggtatc
     2281 cagcgcacga gcggcaagca acgttgacca gtgatgctct ttgagcggcc cacgaaccca
     2341 ggcggcagga agtaccagta tttcagctcc ctgtaatgcc tgtgccagcg ccagctctgg
     2401 aaagcgtaag tcataacagg tcatcagacc gaccttcatc ccctccacct ccagtaacgg
     2461 agctatttca ttaccagcat caacatggcg tgattcctga atggcaaatg catcatagag
     2521 atgcagtttg gcataacggg cgacgatgtt tcctgcctgt agcgccacca gcatattcca
     2581 tgcgcgcccc ggtgttgaag gaacatgaat cgtcagaatt gtcgtcatca tgttacgttt
     2641 actttctcgc cgtaaacgac cgaggaattc gccttccagc agttgtgctg atttaaccga
     2701 tagatctgca tcatgatcat cacgcgccag taatgcttcc ggcaggacaa acagcgaaac
     2761 ctcgttttcc gccgcctgcg ccatcaacga ggcacaaatc tcaacgttct tttcccacac
     2821 agatgtaaca gcaaactgtc ctgcggcaac cagcattgcc acctcctgtc agagtcgtaa
     2881 aattcgccaa cggcgattat tcggcgttac acttatcact catacaaatc aaatagcagg
     2941 attttgcagt gttacaactt cttttagcag tttttattgg cggtggtacg ggaagcgtgg
     3001 cgagatggct gttaagtatg cgatttaacc cgctgcatca ggcgattccg ttgggaacgc
     3061 tggcagcaaa cctgattggg gcattcatca taggaatggg attcgcctgg ttcagcagga
     3121 tgacgaacat tgatccagtg tggaaagtat taatcaccac cggattttgt ggcggtctaa
     3181 caaccttctc aacattttcg gcagaagtgg tgtttttgtt acaagagggc cgctttggct
     3241 gggcgttact gaacgttttc gtcaaccttt tggggtcttt tgccatgacc gcactggctt
     3301 tctggctgtt ttccgcctca accgcacact aaagaccaaa aaaaacccgc tgattaagcg
     3361 ggttttgaat tcttgctgac gtatcttaca gagcgattac gtttgcagca gaagggcctt
     3421 tggcaccgtt agtgatttcg aactctacgc gctgaccttc agcaagagtt ttaaaaccat
     3481 tagtctggat tgcagagaag tgtacgaaca cgtctttgct gccgtcttcc ggagtaatga
     3541 aaccgaatcc tttggactca ttaaaccact taacgttacc tttaatctta gacatcaaaa
     3601 ttacctttac atgaaaaata gacacaaatg ctgtgtcggt taccagtaca ccaattgtgg
     3661 tacgctttgt ccagtgcaac tttactaaaa agtgataaaa gtcgcgttat ttttgatgtt
     3721 aattatttta gtggttgatt ttaaagaatt tactaaaact tcatttgtct caaaactgaa
     3781 agcgcatcca ggcaaagtac acattgccat tgttgtaggt acccggaatg taggtcatct
     3841 gaaacgtcgc ggggccatac cccacagagg ccaatggcag tagaaccgga agagggatgt
     3901 aattccagtt atcgcgcgcc gttacgccag cggtaaatcc caggcctaaa tgaaaatttt
     3961 catccgccag cggtcgccag gtactttccc atccgtatcc ggcaatcggt tcccatttgt
     4021 tccacgagtc cttaaatgcc attgcataca ggccatgcca gtttcctttt tcatcccaac
     4081 gcgactgacc aaaaccgcca ccccacggtc gctcgttata gcgatcggtt ttttctttgt
     4141 cataggcaaa acgcgcatgc caggtgatgg caggaatata taaatcataa tgttctggct
     4201 gttgccaggt ttgtgcaata ttttctctaa acgttgtcat ccactcatca gcgttagcaa
     4261 aaactttacc aacactgatt aactgaataa aaacaaagaa aaatatagcg acatatttac
     4321 tcacgttcat ttgtgaccat taaacattta tcaaaaatct actactaaca tagcaaaact
     4381 acaattaaca taacct
//
LOCUS       DAHHTG010000226         4278 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.230, whole genome shotgun sequence.
ACCESSION   DAHHTG010000226 DAHHTG010000000
VERSION     DAHHTG010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4278)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4278)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4278
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.230"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            complement(<1..299)
                     /gene="tuf"
                     /locus_tag="NBI74_004440"
     CDS             complement(<1..299)
                     /gene="tuf"
                     /locus_tag="NBI74_004440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HCD1934664.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVEYDTPTRHYAHVDCPGHADYVKNMITGAAQM"
     gene            complement(414..489)
                     /locus_tag="NBI74_004441"
     tRNA            complement(414..489)
                     /locus_tag="NBI74_004441"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(454..456),aa:Thr,seq:ggt)
     gene            complement(496..570)
                     /locus_tag="NBI74_004442"
     tRNA            complement(496..570)
                     /locus_tag="NBI74_004442"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(535..537),aa:Gly,seq:tcc)
     gene            complement(687..771)
                     /locus_tag="NBI74_004443"
     tRNA            complement(687..771)
                     /locus_tag="NBI74_004443"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(735..737),aa:Tyr,seq:gta)
     gene            complement(780..855)
                     /locus_tag="NBI74_004444"
     tRNA            complement(780..855)
                     /locus_tag="NBI74_004444"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(820..822),aa:Thr,seq:tgt)
     gene            1217..2167
                     /gene="coaA"
                     /locus_tag="NBI74_004445"
                     /gene_synonym="panK"
     CDS             1217..2167
                     /gene="coaA"
                     /locus_tag="NBI74_004445"
                     /gene_synonym="panK"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HCD1934665.1"
                     /translation="MSIKEQTLMTPYLQFDRNQWAALRDSVPMTLSEDEIARLKGINE
                     DLSLEEVAEIYLPLSRLLNFYISSNLRRQAVLEQFLGTNGQRIPYIISIAGSVAVGKS
                     TTARVLQALLSRWPEHRRVELITTDGFLHPNQVLKERGLMKKKGFPESYDMHRLVKFV
                     SDLKSGVPNVTAPVYSHLIYDVIPDGDKTVVQPDILILEGLNVLQSGMDYPHDPHHVF
                     VSDFVDFSIYVDAPEDLLQTWYINRFLKFREGAFTDPDSYFHNYAKLTKEEAIKTAMT
                     LWKEINWLNLKQNILPTRERASLILTKSANHAVEEVRLRK"
     gene            complement(2196..3161)
                     /gene="birA"
                     /locus_tag="NBI74_004446"
     CDS             complement(2196..3161)
                     /gene="birA"
                     /locus_tag="NBI74_004446"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     ligase/biotin operon repressor BirA"
                     /protein_id="HCD1934666.1"
                     /translation="MKDNTVPLKLIALLANGEFHSGEQLGESLGMSRAAINKHIQTLR
                     DWGVDVFTVPGKGYSLPEPIQLLNAEQILGQLDGGSVTVLPVIDSTNQYLLDRIGELK
                     SGDACVAEYQQAGRGRRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEV
                     LRKLGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESV
                     VNQGWITLQEAGINLDRNSLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPV
                     KLIIGDKEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     gene            complement(3158..4186)
                     /gene="murB"
                     /locus_tag="NBI74_004447"
     CDS             complement(3158..4186)
                     /gene="murB"
                     /locus_tag="NBI74_004447"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HCD1934667.1"
                     /translation="MNHSLKPWNTFGIDHNAQHIVCAEDEQQLLNAWQHATAEGQPVL
                     ILGEGSNVLFLEDYRGTVIINRIKGIEIHDEPDAWYLHVGAGENWHRLVKYTLQEGMP
                     GLENLALIPGCVGSSPIQNIGAYGVELQRVCAYVDCVELATGKQVRLTAKECRFGYRD
                     SIFKHEYQDRFAIVAVGLRLPKEWQPVLTYGDLTRLDPTTVTPQQVFNAVCHMRTTKL
                     PDPKVNGNAGSFFKNPVVSAETAKVLLAQFPTAPNYPQADGSVKLAAGWLIDQCQLKG
                     MQMGGAAVHRQQALVLINEDNAKSEDVVQLALHVRQKVGEKFNVWLEPEVRFIGASGE
                     VSAVETIS"
BASE COUNT         1109 a         1054 c          960 g         1155 t
ORIGIN      
        1 tccatctgag cagcaccggt gatcatgttt ttaacatagt cggcgtgccc cgggcagtct
       61 acgtgtgcgt agtgacgggt cggggtgtcg tattcaacgt gagaagtgtt gatggtgata
      121 ccacgagcct tttcttccgg cgcgttatcg atctggtcga atgcgcgagc agcaccgccg
      181 taggttttag ccagtacggt agtgattgca gcggtcagcg ttgttttacc atggtcaacg
      241 tggccgatag taccgacgtt aacgtgcggt tttgtacgtt caaacttttc tttagacatc
      301 gattgtccct ctaagacacg gataaatcgg tgatatcacc acatcaacca ggcaacatgc
      361 ccgacttgtt gaatgcaata aacagaagga aaaaacaggg aggagaaaag aagtggtgct
      421 gataggcaga ttcgaactgc cgacctcacc cttaccaagg gtgcgctcta ccaactgagc
      481 tatatcagca catcttggag cgggcagcgg gaatcgaacc cgcatcatca gcttggaagg
      541 ctgaggtaat agccattata cgatgcccgc atcctggaac tcggctacct gattttcatt
      601 ctgcactgaa tatcgagaga agctctcttt attcgagccg gtaagcgaac ttatcgtctc
      661 gggctacgcc atcgcgtggc cgaaattggt ggtgggggaa ggattcgaac cttcgaagtc
      721 tgtgacggca gatttacagt ctgctccctt tggccgctcg ggaaccccac cggacttgat
      781 ggtgccgact accggaatcg aactggtgac ctactgatta caagtcagtt gctctaccta
      841 ctgagctaag tcggcatcaa gtagcgcgca ttctatggag acatgcgagt tcatgcaact
      901 aaaaaattgc ataatttgtt ttattggtca cattttatgc gacacgatga agaaacagcc
      961 gaacggaggg cagattagca cactttttca acatcattgt gctcaacaat gtgctcctgc
     1021 taaaccataa ttctttttat cagatggaat atctgtcaca ttgcttttca acgatagctt
     1081 cctgggagag attttttctt attattcctc cccatctggt gttaccctcc tgcccattaa
     1141 cccattcaac agaactgtga cgcgccatgg caaatatcgc tttgccgata gagctatgac
     1201 cgccagaaac atgcttatga gtataaaaga gcaaacgtta atgacgcctt acctacagtt
     1261 tgaccgcaac cagtgggcag ctctgcgtga ttccgtacct atgacgttat cggaagatga
     1321 gatcgcccgt ctcaaaggta ttaatgaaga tctctcgtta gaagaagttg ccgagatcta
     1381 tttacctctg tcacgtttgc tgaacttcta tataagctcg aatctgcgcc gtcaggcagt
     1441 tttggaacag tttcttggta ccaacgggca acgcattcct tacattatca gtattgctgg
     1501 tagtgtcgcg gtggggaaaa gtacaaccgc ccgtgtattg caggcgctat taagccgttg
     1561 gccggaacat cgtcgtgttg aactgatcac tacagatggc ttccttcacc ctaatcaggt
     1621 tctgaaagaa cgtggtctga tgaagaagaa aggcttcccg gaatcgtatg atatgcatcg
     1681 cctggtgaag tttgtttccg atctcaaatc cggcgtgcca aacgttacag cccctgttta
     1741 ctcgcatctt atttatgatg tgatcccgga tggagataaa acggttgttc agcctgatat
     1801 tttaattctt gaagggttaa atgtcttaca gagcgggatg gattatccac acgatccaca
     1861 tcatgtattt gtttctgatt ttgtcgattt ttcgatatat gttgatgcac cggaagactt
     1921 acttcagacg tggtatatca accgttttct gaaattccgc gaaggggctt ttacagaccc
     1981 ggattcctat tttcataact acgcgaaatt aactaaagaa gaagctatta agactgccat
     2041 gacattgtgg aaagagatca actggctgaa cttaaagcaa aatattctac ctactcgcga
     2101 gcgtgccagt ttgatcctga cgaaaagtgc taatcatgca gtagaagagg tcagactacg
     2161 caaataattt tcaggggagc taatgctccc ctttcttatt tttctgcact acgcagggat
     2221 atttcaccgc ccatccaggg ttttattatt ccatcctgct caagtaataa agcaccctgt
     2281 ttgtctattc cgcgtgaaat gccaaatatt tctttatcac caatgataag tttcactggg
     2341 cgattaataa aattatccaa cttttcccag cgcgaaagat aaggtgccaa tccttcttgc
     2401 tcgaagagtt ccagcgccgc gcgtaattca cgtatcagca tggccgcaag actattacga
     2461 tcgagattga tcccggcttc ctgcagcgtg atccaccctt gattaacgac actctcttca
     2521 acacgacgca ttgccatgtt gatccctgcg ccaatgacta tttgcgccgc atcgccggtt
     2581 ttgccagtca gctccacaag aatgcctgcc agcttgcgat cctgcagata gaggtcatta
     2641 ggccatttaa cacgaacttt atctgcaccc agcttgcgta atacttccgc catcacgata
     2701 ccgataacca gacttaaacc aatcgccgcc gccgggcctt gttccagacg ccagaacatc
     2761 gacaaatata agtttgcgcc aaaaggcgaa aaccatttcc gaccccggcg accacggcca
     2821 gcctgctggt attctgcaac acaggcatcg cccgatttaa gctctccgat acgatcaaga
     2881 aggtactgat tcgtggagtc aataacgggc agcacagtta cactaccgcc atccagctga
     2941 cccaatatct gttcagcatt aagtaactgg ataggctcag gcaggctgta tcctttaccc
     3001 ggaacggtaa agacatcaac gccccagtca cgcagtgtct gaatgtgttt attaatagcc
     3061 gcccggctca ttcctaacga ttcacccaac tgctcaccag agtgaaattc gccattcgct
     3121 aacagcgcga ttaatttcag cggaacggta ttatccttca tgaaatcgtc tccactgcgc
     3181 taacttcacc tgatgcacca ataaagcgaa cttcaggctc aagccagaca ttaaattttt
     3241 cacccacttt ttgtcttaca tgaagtgcca gttgcaccac atcttcgctt tttgcattgt
     3301 cttcattaat gaggactaac gcctgttgac ggtgcaccgc agccccaccc atttgcatcc
     3361 cttttagctg gcactgatcg ataagccaac ctgctgccag ttttactgaa ccatccgcct
     3421 ggggataatt tggtgctgtt ggaaattgtg ccagtaatac tttagccgtt tcggcagata
     3481 caacagggtt tttgaagaaa ctaccggcat tgccattcac ttttggatca gggagtttgg
     3541 tggtgcgcat atgacacacc gcattaaata cttgctgtgg cgttactgtt gtaggatcca
     3601 gacgagttaa gtcaccatac gtcagtacag gttgccactc ttttggcaga cgcaggccta
     3661 cggctacaat agcgaagcga tcctggtatt catgtttaaa aatactgtcg cgatagccaa
     3721 aacggcactc tttggcagtt aagcgcactt gcttgcctgt cgccagttca acacaatcaa
     3781 cataagcgca aactcgctgt aattctacgc cataagcacc aatattctgg ataggtgatg
     3841 agccgacaca accaggaatt aatgccagat tttccagacc aggcatacct tcctgcaaag
     3901 tgtattttac aaggcgatgc cagttttctc cggctcctac atgtaaatac cacgcatcag
     3961 gttcatcatg aatttcgata cctttgatcc ggttgatgat caccgtgccg cgatagtctt
     4021 ccagaaaaag tacattactt ccttcaccca gaataagaac gggttgtcct tctgcggttg
     4081 catgctgcca ggcattgagt aattgttgtt cgtcttcggc acatacaatg tgctgagcat
     4141 tatgatcaat gccaaatgtg ttccagggtt ttaaggagtg gttcatagct gctttcctga
     4201 tgcaaaaacg aggctagttt accgtatctg tggggggatg gcttgtagat atgacgacag
     4261 gaagagttta tagaaacg
//
LOCUS       DAHHTG010000227         4199 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.231, whole genome shotgun sequence.
ACCESSION   DAHHTG010000227 DAHHTG010000000
VERSION     DAHHTG010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4199)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4199)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4199
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.231"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            complement(<1..557)
                     /locus_tag="NBI74_004448"
     CDS             complement(<1..557)
                     /locus_tag="NBI74_004448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HCD1934668.1"
                     /translation="MGLFTTRQLLGYTEQKVKFRALFLELFFRRTVNFHTEEVMLDKI
                     TGKTPVAAYVSPVVEGKVLRHRGGETRVLRPGYVKPKHEFNYQQAVERLPGEDPSQLN
                     DPAYRRLRIITDNLKQEEHAIVQVEEMQAVNAVLYGKYTMEGDQFEKIEVDFGRSTKN
                     NITQGSGKEWSKQDRDTFDPTHDIDL"
     gene            complement(615..962)
                     /locus_tag="NBI74_004449"
     CDS             complement(615..962)
                     /locus_tag="NBI74_004449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head decoration protein"
                     /protein_id="HCD1934669.1"
                     /translation="MVTKTITEQRAEVRIFAGNDPAHTATGSSGISSATPALTPLMLD
                     EATGKLVVWDGQKAGSAVGILVLPLEGTETALTYYKSGTFATEAIHWPESVDEHKKAN
                     AFAGSALSHAALP"
     gene            complement(999..2504)
                     /locus_tag="NBI74_004450"
     CDS             complement(999..2504)
                     /locus_tag="NBI74_004450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HCD1934670.1"
                     /translation="MRRNLSHIIAAAFNEPLLLEPAYARVFFCALGREMGAASLSVPQ
                     QQVQFDALGMLAETDEYMAGGKRPARVYRVVNGIAVLPVTGTLVHRLGGMRPFSGMTG
                     YDGIVACLQQAMADSQVRGVLLDIDSPGGQAAGAFDCADMIYRLRQQKPVWALCNDTA
                     CSAAMLLASACSRRLVTQTSRIGSIGVMMSHVSYAGHLAQAGVDITLIYSGAHKVDGN
                     QFEALPAEVRQDMQQRIDAARRMFAEKVAMFTGLSVDAVTGTEAAVFEGQSGIEAGLA
                     DELINASDAISVMATALNSNVRGGTMPQLTATEAAAQENQRVMGILTCQEAKGREQLA
                     TMLAGQQGMSVEQARAILAAAAPQQPVASTQSEADRIMACEEANGREQLAATLAAMPE
                     MTVEKARPILAASPQADAGPSLRDQIMALDEAKGAEVQAEQLAACPGMTVECARAVLA
                     AGSGKAEPVSASTTAMFEHFMANHSPAAVQGGVPQTSADGDADVKMLMAMP"
     gene            complement(2494..4086)
                     /locus_tag="NBI74_004451"
     CDS             complement(2494..4086)
                     /locus_tag="NBI74_004451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HCD1934671.1"
                     /translation="MKRTPVLIDVNGVPLRESLSYNGGGAGFGGQMAEWLPPAQSADA
                     ALLPALRLGNARADDLVRNNGIAANAVALHKDHIVGHMFLISYRPNWRWLGMRETAAK
                     SFVDEVEAAWSEYAEGMFGEIDVEGKRTFTEFIREGVGVHAFNGEIFVQPVWDTETTQ
                     LFRTRFKAVSPKRVDTPGHGMGNPFLRAGVEVDRYGRAVAYHICEDDFPFSGSGRWER
                     IPRELPTGRPAMLHIFEPVEDGQTRGANQFYSVMERLKMLDSLQATQLQSAIVKAMYA
                     ATIESELDTEKAFEYIAGAPQEQKDNPLINILEKFSSWYDTNNVTLGGVKIPHLFPGD
                     DLKLQTAQDSDNGFSALEQALLRYIAAGLGVSYEQLSRDYSKVSYSSARASANESWRY
                     FMGRRKFIAARLATQMFSCWLEEALLRGIIRPPRARFDFYQARSAWSRAEWIGAGRMA
                     IDGLKEVQESVMRIEAGLSTYEKELALMGEDYQDIFRQQVRESAERQKAGLSRPVWIA
                     QAYQQQIAESRRPEEETTPRET"
     gene            complement(4083..>4199)
                     /locus_tag="NBI74_004452"
     CDS             complement(4083..>4199)
                     /locus_tag="NBI74_004452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCD1934672.1"
                     /translation="RRIEYTAASLDELNRAINDAESVLGTTRCRRRPLGVRL"
BASE COUNT          895 a         1338 c         1025 g          941 t
ORIGIN      
        1 aggtcaatat catgtgtagg atcgaacgtg tcacgatcct gttttgacca ctccttacca
       61 ctaccctgag tgatgttatt cttcgtcgac ctgccaaaat cgacctcaat tttctcgaac
      121 tggtctcctt ccatggtgta tttgccatac aacacagcat ttaccgcctg catttcttcc
      181 acctggacaa tcgcgtgctc ttcctgtttg aggttatcgg taatgatacg cagacggcgg
      241 taagccggat cattcagttg agatggatct tcaccaggaa ggcgctcaac cgcctgctgg
      301 taattaaatt cgtgtttcgg cttgacgtag cccggacgca acacgcgggt ttcaccacca
      361 cgatgacgca gcacttttcc ttcaacaacc ggggagacat aggccgccac cggcgttttt
      421 ccggtaattt tgtccagcat cacctcttcg gtatggaaat tcaccgtacg gcggaaaaac
      481 agttccagaa acagcgcacg aaatttaact ttttgttcgg tataaccgag taactggcgg
      541 gtcgtaaaca atcccataaa tcagttcctt tcattcagaa atcagtcagg ccagcgcggt
      601 ggcctgataa cgtgttacgg cagcgccgcg tgactcaggg cactgccagc aaaggcgttg
      661 gcctttttgt gttcatccac actttcaggc cagtggattg cctccgtcgc aaaggttccc
      721 gacttgtaat acgtcagcgc cgtctctgtg ccttcaagcg gcagtaccag tatgccaacc
      781 gcactaccgg ctttctgtcc gtcccagacc accagtttcc cggtggcttc atccagcatc
      841 aggggcgtca gagccggtgt tgcagaagaa atcccgctgc tgcctgtggc ggtatgagcc
      901 ggatcattac cggcaaaaat acgtacttcc gcacgctgtt cagtgatggt tttcgtcacc
      961 atattgttaa aacctcctat tgatggtcag cactggcttc atggcatggc catgagcatt
     1021 ttcacgtccg catcaccgtc tgctgacgtc tgtggcacgc caccctgtac cgctgccggt
     1081 gaatggttcg ccatgaaatg ttcaaacatg gcggttgtgg atgcagagac cggttctgcc
     1141 ttacctgatc ccgcagccag cacagcccgg gcgcactcca cagtcattcc cgggcaggca
     1201 gccagctgtt cagcctgcac ctcagcccct tttgcctcat ccagtgccat gatctgatca
     1261 cggagtgatg gtccggcatc cgcctgcggt gaagcagcca ggatcgggcg ggctttttcc
     1321 accgtcatct ccggcatcgc cgccagcgtt gccgccagtt gttcacgacc gttcgcttct
     1381 tcacacgcca taatgcgatc ggcttcactc tgcgtggatg ccaccggctg ctgcggtgcc
     1441 gccgcggcca gaatcgcccg ggcctgttca acgctcatgc cctgttgtcc tgccagcatc
     1501 gtggcaagct gttcacgtcc tttcgcttcc tggcatgtca ggatccccat cactcgctgg
     1561 ttctcctgcg cggcggcttc cgttgcagtt aattgcggca tagtgcctcc tctgacatta
     1621 ctgttcagcg ccgtggccat cacactgatg gcatccgacg cattgattaa ttcatccgcc
     1681 agcccggcct caatgccgga ctgaccttca aaaacagcgg cctctgttcc tgtgactgca
     1741 tcaacagaca gaccggtaaa catcgccact ttttcggcaa acatccggcg cgccgcatca
     1801 atccgctgct gcatgtcctg gcgaacctct gccggcaacg cttcaaactg attgccatcc
     1861 accttgtgcg cccctgagta aatcagcgtg atatccacac cggcctgcgc cagatgaccg
     1921 gcatagctga catggctcat catcacgcca atggaaccga tacgggatgt ctgggtaacc
     1981 agccgtcggg agcaggccga cgccagcagc atggctgcag aacaggccgt gtcattgcac
     2041 agtgcccaga ccggcttctg ctgacggagg cggtaaatca tgtcagcgca gtcaaacgcg
     2101 ccggcggcct gcccgcccgg actgtcaatg tccagcagta cgccccgcac ctggctatcc
     2161 gccattgcct gctgaagaca ggcgacaata ccgtcatagc ctgtcattcc ggaaaatggc
     2221 cgcataccac ccagccggtg caccagcgtg ccggtcaccg gcagtacagc aataccgttc
     2281 accaccctgt aaacacgggc cggtcgttta cctccggcca tgtactcgtc cgtttcagcc
     2341 agcattccga gagcatcaaa ctgtacctgc tgttgtggta ccgaaagact tgctgccccc
     2401 atctcgcgcc cgagcgcgca aaagaaaacc cgcgcatagg cgggctccag aagcagcggt
     2461 tcattgaatg ctgcggcaat aatgtgtgaa agattacgtc tcacgtggtg ttgtctcctc
     2521 ttccggccta cgactctccg ctatctgctg ctgatacgcc tgcgctatcc acaccggacg
     2581 tgagagtccg gctttttgcc gctcagcaga ttccctgacc tgctggcgga aaatgtcctg
     2641 ataatcctcg cccatcagcg ccagctcttt ctcatacgtg ctcagtccgg cctcaatgcg
     2701 catcactgat tcctggactt ccttgagccc gtcaatggcc attcttccgg caccaatcca
     2761 ctctgcccgt gaccaggctg atcgcgcctg ataaaaatca aaacgtgccc gtggcggacg
     2821 aataatcccc cgaagaagtg cctcttccag ccagcaggaa aacatctgcg tggccagccg
     2881 ggccgcaata aattttcgcc gccccataaa atagcgccac gactcattgg cggaggcgcg
     2941 ggcacttgag taactgacct tcgagtaatc acgggacaac tgttcgtagg aaacgccaag
     3001 accggcggcg atataccgca gcagcgcctg ttcaagcgca gaaaatccat tgtctgaatc
     3061 ctgcgcagtc tgtagtttca gatcatcacc agggaaaagg tgcggaattt tgacaccgcc
     3121 cagtgtcacg ttattcgtgt cataccagct ggaaaacttc tccagaatat taataagcgg
     3181 attatccttc tgctcctgtg gcgcgccggc gatatattca aaggcctttt cggtatcaag
     3241 ttcactttca atcgtcgctg catacatcgc cttcactatg gccgactgaa gctgtgttgc
     3301 ctgcagggaa tcgagcatct tcagccgttc catgacgctg taaaactgat tagccccacg
     3361 ggtctgcccg tcctccaccg gctcgaaaat atgcaacatg gccggacgcc cggtgggaag
     3421 ttcacgcggg atccgttccc atcgtccact accagagaac ggaaaatcat cctcacagat
     3481 atggtacgcg acggcacggc catatcgatc gacctccaca ccggcccgca gaaaagggtt
     3541 ccccataccg tgtccaggcg tgtccacccg tttcggactc acggctttaa aacgcgtacg
     3601 gaataactgc gtggtttccg tatcccagac cggctgcaca aagatttcgc cgttaaacgc
     3661 atgaacgccc acaccttcac ggataaattc cgtgaacgtg cgttttcctt ccacgtcgat
     3721 ctcgccaaac atcccttcgg cgtattccga ccaggccgcc tccacctcat cgacaaagct
     3781 ttttgctgcg gtctcccgca tccccagcca gcgccagttc ggacggtagc tgatcagaaa
     3841 catatgcccg acaatgtgat ccttatgcag agccaccgca ttagccgcta ttccgttatt
     3901 gcgcaccaga tcatctgccc gggcattccc cagacgcaac gcgggcagca gggccgcatc
     3961 ggcactctgc gccggtggca accactcagc catttgcccg ccaaatcctg caccgccccc
     4021 gttgtagctg agactctcac gaagcggaac gccgttcaca tcaatcagga caggcgttcg
     4081 tttcataacc tcactcccag cggacgacgg cgacaccggg ttgtccccag taccgactcc
     4141 gcatcattga tcgcccggtt aagctcatcc agagaagccg ccgtatattc aattctgcg
//
LOCUS       DAHHTG010000228         4155 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.232, whole genome shotgun sequence.
ACCESSION   DAHHTG010000228 DAHHTG010000000
VERSION     DAHHTG010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4155)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4155)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4155
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.232"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            complement(70..723)
                     /locus_tag="NBI74_004453"
     CDS             complement(70..723)
                     /locus_tag="NBI74_004453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001757010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HCD1934673.1"
                     /translation="MIQTRNQYLQFMLVMLAAWGISWGARFVMEQAVLLYGSGKNYLF
                     FSHGTVLMYLLCVFLVYRRWIAPLPVVGQLRNVGVPWLVGAMAVVYVGVFLLGKALAL
                     PAEPFMTKLFADKSIPDVILTLLTIFILAPLNEETLFRGIMLNVFRSRYCWTMWLGAL
                     ITSLLFVAAHSQYQNLLTLAELFLVGLITSVARIRSGGLLLPVLLHMEATTLGLLFG"
     gene            complement(1662..2519)
                     /gene="repA"
                     /locus_tag="NBI74_004454"
     CDS             complement(1662..2519)
                     /gene="repA"
                     /locus_tag="NBI74_004454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="incFII family plasmid replication initiator
                     RepA"
                     /protein_id="HCD1934674.1"
                     /translation="MTDLQQTYYRQVKNPNPVFTPRKGAGTLKFCEKLMEKAVGFTSR
                     FDFAIHVAHARSRGLRRRMPPVLRRRAIDALLQGLCFHYDPLANRVQCSITTLAIECG
                     LATESAAGKLSITRATRALTFLSELGLITYQTEYDPLIGCYIPTDITFTSALFAALDV
                     SEEAAAAARRSRVVWENKQRKKQGLDTLGMDELIAKAWRFVRERFRSYQTELKSRGIK
                     RARARRDADRERQDIVTLVKRQLTREIAEGRFTANREAVKREVERRVKERMILSRNRN
                     YSRLATASP"
     gene            complement(2512..2586)
                     /gene="tap"
                     /locus_tag="NBI74_004455"
     CDS             complement(2512..2586)
                     /gene="tap"
                     /locus_tag="NBI74_004455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001370046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepA leader peptide Tap"
                     /protein_id="HCD1934675.1"
                     /translation="MPGKVQDFFLCSLLLRIVSAGWCD"
     gene            complement(2831..3079)
                     /gene="repA"
                     /locus_tag="NBI74_004456"
     CDS             complement(2831..3079)
                     /gene="repA"
                     /locus_tag="NBI74_004456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000083832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication regulatory protein RepA"
                     /protein_id="HCD1934676.1"
                     /translation="MSQTENAVTSSSGTKRAYRKGNPLTLAERQQASLARKRATHKEL
                     RVFIPAALKAQLQVMCEAEGVTQAEMIAELIKQKSAFS"
     gene            complement(3364..3513)
                     /locus_tag="NBI74_004457"
     CDS             complement(3364..3513)
                     /locus_tag="NBI74_004457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001300273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Hok family toxin"
                     /protein_id="HCD1934677.1"
                     /translation="MTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLA
                     YEARK"
     gene            complement(3760..4035)
                     /locus_tag="NBI74_004458"
     CDS             complement(3760..4035)
                     /locus_tag="NBI74_004458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001472229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1934678.1"
                     /translation="MNSEEQTIIFNCAGWPDLGGGSAVSQFAMMVRKFGKQLIVAVSE
                     KDAIKLKDNFDIIGMLSYGKENFIAMSHTKNELTGGKRRYRIKNGCK"
BASE COUNT         1044 a         1078 c          985 g         1048 t
ORIGIN      
        1 tttaaatata acatccacta aaaaaacaca ccaggcttta acgcacaatg tttaataaaa
       61 atataacttt caaccaaaca gtaaacccag cgtggttgct tccatatgca gcaataccgg
      121 cagcagcagg ccaccacttc tgatcctggc cactgatgta atcaacccca ccaggaacag
      181 ttctgccagt gtcagcaggt tctgatactg gctgtgcgcg gcgacgaaca acaacgacgt
      241 tatcagcgcc cccagccaca tcgtccagca gtaccgtgaa cggaagacgt tcagcataat
      301 cccccggaac agcgtttcct cattcaacgg ggcaaggata aagatggtca gcaacgtcag
      361 gatcacgtca ggtatggact tatcggcaaa aagtttcgtc ataaatggct cagcaggcag
      421 agccagcgcc ttaccgagca gaaatacacc gacatacacc acggccatcg caccgaccag
      481 ccacggtacg ccaacgttgc gcagctgacc aacgaccggt agcggagcta tccaacggcg
      541 gtataccagg aaaacacaca gcaggtacat cagaacagta ccatgactga agaacaaata
      601 gttttttcct gagccataaa gcagaacggc ctgctccatg acaaatctgg ctccccaact
      661 aatgccccat gcagccagca taaccagcat aaactgcaga tattgattac gtgtttgaat
      721 cattgcatcg cctgtaaatt tttaacttgt cctatttttg tcattaccac gtatatacac
      781 atgtataaca attcagatat cgttaccagg atatgccgca tcagcggcat ggaaggcggc
      841 actctgttgt ttcatatgat acaggagtaa aaccgccgaa gcccggcgta agccggtact
      901 gattgataga tttcacctta cccatcccca gccctgccag accatacccg ctttcagcca
      961 tgagagagct tctgtgcgcg gtcggagtgg tcccgacgag ggtttacccg aagtcggggc
     1021 gtatctccgc gttagcgggc cgtgagggcc gcttacgagc gtgtactgag aacttccagc
     1081 gagaagactg acagcgatga agatgtagtt acaacattca taattaaaag cgactctgtt
     1141 ccggcccttt gggccggggc ggggccgctt ttcagttatg agggaggggc tttgtggttt
     1201 cggttctgcg ctggaccggg gtttttctgg aggttgtttt tgtgtgttgt aactaaagtg
     1261 gctccggtcg gggcccgccg cttgcggtgg gaggtgcata tctgtctgtc cacaggacag
     1321 gcagtgaaca ggttttcttt ttaaatgaat gtaattaagt agtttaaagg agatataaac
     1381 aggtgtttaa aagatacatt gcaccctgta agactggcgg ctggcgcttt atgacatgaa
     1441 cggttgtaac cttatgggga agtcccttgc agtttaatgt ggataagcaa aattccccgt
     1501 ctgtgaggcg tgttttgtat cgaaaacagg gggggccgga tgcaccagaa gctggatgat
     1561 gaggttgttt tttgtatgtg gtgctgtttt tttgtgcatc gacgggcttc aggcgtgcga
     1621 atgcctccag cgcgggccgg attattcaga ggaggtcact ttcaggggga agctgtggcc
     1681 agccggctgt aattgcggtt acgtgacaga atcatgcgct ccttcacacg acgctcaact
     1741 tcgcgtttta ccgcctcacg attggcagtg aagcgccctt ccgcgatttc gcgcgtcagc
     1801 tgccgtttca ccagggtgac aatatcctga cgttccctgt ccgcatcacg acgcgcacgg
     1861 gcacgcttta ttccccggga cttaagctct gtctgataac tgcgaaaacg ctcacgaaca
     1921 aaacgccagg ctttcgctat cagttcatcc atgcccaggg tatccagccc ctgctttttg
     1981 cgttgtttgt tttcccatac cacacggctg cggcgcgcgg cggccgctgc ctcctctgat
     2041 acatcgaggg cagcaaacag tgcagatgtg aacgtgatat cggtcggaat gtagcacccg
     2101 ataagcgggt catattccgt ctggtaggta atcagtccca gctctgacag gaacgtcagg
     2161 gcccgggtgg cacgggtgat ggagagtttt ccggcagcag actccgtcgc cagtccgcac
     2221 tcaatggcca gcgtggtgat ggagcactgg acgcggttgg ccagcgggtc atagtggaaa
     2281 cacagcccct gcaggagcgc atcaatagcc cgtcgacgca gcaccggtgg catgcgtcga
     2341 cgcagaccac gcgaacgggc atgcgccaca tgaatggcga aatcaaaacg ggaagtgaag
     2401 cccaccgcct tttccatcag tttttcgcag aacttcagcg ttccggcacc tttacggggt
     2461 gtaaacaccg gattcgggtt ctttacctgg cggtaatacg tttgttgaag atcagtcaca
     2521 ccatcctgca cttacaatgc gcagaaggag tgagcacaga aagaagtctt gaacttttcc
     2581 gggcatataa ctatactccc cgcatagctg aattgttggc tatacggttt ttagatggcc
     2641 ccggtaatct tctcagtcgc caaacttgtc gaagattatc ggggttttca cttttctggc
     2701 tcctgtaaat ccacatcaga accagttccc tgccacctta cggcgtggcc agccacaaaa
     2761 ttccttaaac gatcagcaat ctatcacgca caccagaaac aggcaagaat gtgatgtcgc
     2821 tacattttag ttagctaaaa gcactcttct gctttatcag ttcggcaatc atctcagcct
     2881 gggtaacgcc ctcagcttca cacatcacct gcaattgcgc ttttaacgca gcaggaatga
     2941 acactctgag ttctttatgt gtagctcgtt ttcttgccag ggatgcctgc tgacgttctg
     3001 caagcgtcag aggattacct tttctgtatg ctcgctttgt acctgatgag gaagtcactg
     3061 cattttctgt ctgcgacatc tcgcctcctc aatactcaaa cagggatcgt ttcgcagagg
     3121 atactacagt tttttgaaat cagcgacttg agaattgtga cgaagatctg tgttggcgca
     3181 caaatcaacg gggattactg ttgtttaatg tgatttttgt tgcgaaatgg ctgaaaaaac
     3241 aggaaatgcc ttaacccggg agccggcccc gggcgggacc ggctgtttct ggcatgtcgg
     3301 gtgtctgccc ctgaaatacc tcacacttcc ggaaagcggg gacttccgtc cccgcccggc
     3361 agtttacttc cgtgcttcgt aggccagagt tacctgcacc actgtatttc ccctgtgaat
     3421 attcagctca cataaccgtt ccctgaatat cagtgaaaaa cacaacaccg tggcgcacac
     3481 ggcgagcaac ccgataaggg catacttcgt cataaacttt gacctctctg caaggaagag
     3541 gctacaatca gtagtgcaat gtacttcatg cagacctcac gagttaatgg attaacaagt
     3601 ggggtctttg catttctggt acctgcgtct tgcctgaacc agaaaggctc agacacccgc
     3661 cgcgataata ccacattccc tgttgctgac tattacctgc attgcccgct ttttactaca
     3721 gaagcacgaa ggcgtcactc cagccaataa gtatgctttt tatttacatc catttttaat
     3781 ccggtaccga cgttttcctc cagttaactc gtttttcgta tgactcatgg caataaaatt
     3841 ttctttacca tatgacaaca tcccaataat atcaaagttg tcttttaatt tgatggcatc
     3901 cttttcactt acagcgacaa taagttgttt accaaatttt ctgaccatca ttgcaaactg
     3961 tgatactgct gatccaccac ctaaatcagg ccacccagca caattaaaaa tgattgtctg
     4021 ctcttcacta ttcattgcat ccctgataaa ggagacagca tcctcatttc tctctacatc
     4081 tgaatcactg ttaatctgag tgaaatcata atatttagaa cctggtatag aatacccctt
     4141 aaaaaactta tcaat
//
LOCUS       DAHHTG010000229         4104 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.233, whole genome shotgun sequence.
ACCESSION   DAHHTG010000229 DAHHTG010000000
VERSION     DAHHTG010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4104)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4104)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4104
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.233"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            95..1753
                     /gene="dpiB"
                     /locus_tag="NBI74_004459"
     CDS             95..1753
                     /gene="dpiB"
                     /locus_tag="NBI74_004459"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase DpiB"
                     /protein_id="HCD1934679.1"
                     /translation="MLQLNENKQFAFFQRLAFPLRIFLLILVFSIFVIAALAQYFTAS
                     FEDYLTLHVRDMAMNQAKIIASNDSIISAVKTRDYKRLATIADKLQRDTDFDYVVIGD
                     RHSIRLYHPNPEKIGYPMQFTKPGALEKGESYFITGKGSIGMAMRAKTPIFDDDGKVI
                     GVVSIGYLVSKIDSWRAEFLLPMAGVFVVLLGILMLLSWFLAAHIRRQMMGMEPKQIA
                     RVVRQQEALFSSVYEGLIAVDPHGYITAINRNARKMLGLSSPGRQWLGKPIAEVVRPA
                     DFFTEQIDEKRQDVVANFNGLSVIANREAIRSGDDLLGAIISFRSKDEISTLNAQLTQ
                     IKQYVESLRTLRHEHLNWMSTLNGLLQMKEYDRVLAMVQGESQAQQQLIDSLREAFAD
                     RQVAGLLFGKVQRARELGLKMVIVPGSQLSQLPPGLDSTEFAAIVGNLLDNAFEASLR
                     SDEGNKIVELYLSDEGDDVVIEVADQGCGVPESLRDKIFEQGVSTRADEPGEHGIGLY
                     LIASYVTRCGGVITLEDNDPCGTLFSIYIPKVKPNDSSINPIDR"
     gene            1722..2402
                     /gene="dpiA"
                     /locus_tag="NBI74_004460"
                     /gene_synonym="citB"
     CDS             1722..2402
                     /gene="dpiA"
                     /locus_tag="NBI74_004460"
                     /gene_synonym="citB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component response regulator DpiA"
                     /protein_id="HCD1934680.1"
                     /translation="MTAPLTLLIVEDETPLAEMHAEYIRHIPGFSQILLAGNLAQARM
                     MIERFKPGLILLDNYLPDGRGINLLHELVQAHYPGDVVFTTAASDMETVSEAVRCGVF
                     DYLIKPIAYERLGQTLTRFRQRKHMLESIDSASQKQIDEMFNAYARGEPKDELPTGID
                     PLTLNAVRKLFKEPGVQHTAETVAQALTISRTTARRYLEYCASRHLIIAEIVHGKVGR
                     PQRIYHSG"
     gene            complement(2443..3828)
                     /gene="dcuC"
                     /locus_tag="NBI74_004461"
     CDS             complement(2443..3828)
                     /gene="dcuC"
                     /locus_tag="NBI74_004461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HCD1934681.1"
                     /translation="MLTFIELLIGVVVIVGVARYIIKGYSATGVLFVGGLLLLIISAI
                     MGHKVLPSSQASTGYSATDIVEYVKILLMSRGGDLGMMIMMLCGFAAYMTHIGANDMV
                     VKLASKPLQYINSPYLLMIAAYFVACLMSLAVSSATGLGVLLMATLFPVMVNVGISRG
                     AAAAICASPAAIILAPTSGDVVLAAQASEMSLIDFAFKTTLPISIAAIIGMAIAHFFW
                     QRYLDKKEHISHEMLDVSEITTTAPAFYAILPFTPIIGVLIFDGKWGPQLHIITILVI
                     CMLIASILEFIRSFNTQKVFSGLEVAYRGMADAFANVVMLLVAAGVFAQGLSTIGFIQ
                     SLISIATSFGSASIILMLVLVILTMLAAVTTGSGNAPFYAFVEMIPKLAHSSGINPAY
                     LTIPMLQASNLGRTLSPVSGVVVAVAGMAKISPFEVVKRTSVPVLVGLVIVIVATELM
                     VPGTAAAVTGK"
BASE COUNT         1125 a          961 c         1047 g          971 t
ORIGIN      
        1 aatttgccac gatcatattt aatatctatg tatttttatt caacatttta attacatccg
       61 tcaaagaggc tcgggacaac ccgcaaggaa aacaatgttg cagcttaacg agaataaaca
      121 gtttgcattt ttccaaagac tggcatttcc gctgcgtatc tttttgctga ttctggtgtt
      181 ctcaatattt gtcattgcag ccctggcgca atattttacg gccagttttg aggactattt
      241 aacgcttcat gtgcgcgaca tggcaatgaa tcaggcaaaa attattgctt ctaatgacag
      301 tatcatcagc gcagtgaaaa cgcgtgacta caagaggctg gcgaccatcg ctgacaaatt
      361 acaaagagat actgatttcg attatgtggt gattggcgac cggcactcga ttcgtcttta
      421 ccatcctaat ccggagaaaa ttggttatcc tatgcagttc accaaaccgg gcgcgctgga
      481 gaaaggggag agctacttca tcaccgggaa agggtcaatt ggcatggcga tgcgtgccaa
      541 aacgccaatc tttgatgacg atggaaaagt catcggtgtg gtgtcgattg gctatctggt
      601 gagtaaaatc gatagctggc gggctgagtt tttattaccg atggcaggcg tgtttgtcgt
      661 gctgttaggg attttgatgt tgctatcgtg gttcctggcc gcgcatatcc gtcggcagat
      721 gatgggcatg gagccaaagc aaatcgcacg cgtggtccgt cagcaagagg cgctgtttag
      781 ttcggtttat gaagggctga ttgcggtgga tccgcacggg tacattaccg ccatcaatcg
      841 taacgcaaga aagatgctgg ggctgagctc ccccggacgg caatggttgg gtaaacccat
      901 tgctgaagtg gtcaggcccg ccgatttctt taccgaacag attgatgaaa aacgtcagga
      961 tgtggtggcg aactttaacg gtctgagcgt tatcgccaat cgggaagcta ttcgttctgg
     1021 cgatgatttg ctgggggcca ttatcagctt tcgtagtaaa gacgaaattt ccaccctcaa
     1081 tgcgcaactg acgcaaatta aacaatacgt cgagagcctt cgtacattgc gacacgagca
     1141 tctcaattgg atgtcgacgc tcaatggtct gttgcagatg aaagagtatg atcgcgtgct
     1201 ggcgatggtg cagggtgagt ctcaggccca gcaacagctt attgacagcc tgcgtgaggc
     1261 gtttgccgat cgccaggtgg cggggctgct ttttggtaaa gtgcagcgcg cccgcgaact
     1321 ggggctaaaa atggtcatcg tccccggtag ccagctttcg caactgccgc caggactgga
     1381 cagcaccgag tttgcagcca ttgttggcaa tttacttgat aacgccttcg aagcaagcct
     1441 gcgtagcgat gaaggaaaca agattgttga attatacctc agcgatgaag gcgatgatgt
     1501 ggtgattgaa gtcgccgatc agggctgcgg cgttccagag tctctacgag acaaaatatt
     1561 tgagcagggt gtcagtacgc gtgctgacga gcccggtgaa catggcattg ggttgtactt
     1621 gattgccagc tacgtaacgc gctgcggtgg tgttatcact ctcgaagata atgatccctg
     1681 cggtacctta ttttcaatct atattccgaa agtgaaacct aatgacagct ccattaaccc
     1741 tattgatcgt tgaggacgaa acgccgctgg cagagatgca tgcggaatat attcgtcaca
     1801 ttcccggatt cagccagata ttactggcgg gaaacctggc gcaggcccga atgatgatcg
     1861 agcgttttaa gccggggcta atcttgctcg ataactatct tcctgacggt agagggatta
     1921 atttactgca tgaactggtg caggcgcatt atcccggcga cgtggtgttt accactgcag
     1981 ccagcgatat ggaaacggtg tctgaagccg tacgttgtgg tgtatttgat tatctcatta
     2041 agccaattgc ctatgaacgt ctggggcaaa cgctaacccg tttccgccag cgtaaacata
     2101 tgctggaaag tattgatagc gccagccaga agcaaattga tgagatgttt aatgcctatg
     2161 ctcgcggtga acccaaagac gaactgccga ccggcattga ccccttgacg ctaaacgcgg
     2221 tgcgaaaact gttcaaagag cctggtgtgc aacatacggc agaaacggtg gcgcaggcac
     2281 tgaccatcag tcgcaccact gccaggcgtt atcttgaata ttgcgccagc cgccatctga
     2341 ttattgctga aattgttcac ggcaaagttg gcagaccgca acgcatatac cacagtgggt
     2401 gacatgataa agccggagga aacttgcctc cggcattact atttacttgc ctgtgaccgc
     2461 tgctgccgtt cctggcacca tcagctctgt agcaacgata acaatcacca aaccaacaag
     2521 caccggcacc gaggtgcgtt ttacgacttc aaacggcgag atcttcgcca tcccggcaac
     2581 cgcaacgact acgccagaaa cgggtgatag ggtacgaccc aggtttgacg cctgcagcat
     2641 cgggatagtc aaatacgccg ggttaatgcc ggaggagtgc gccagtttcg ggatcatctc
     2701 aacaaacgca taaaacggcg cattgcctga accggtcgtg actgccgcca gcattgtcag
     2761 gatcaccaat accagcatca ggatgatact cgccgaacca aacgaggtag cgatagaaat
     2821 cagactttga ataaagccga tggtgctaag cccctgagcg aataccccag cggcaaccag
     2881 cagcatcacc acgttagcaa atgcatctgc cataccgcga taagccactt ccagaccaga
     2941 gaaaactttc tgggtattaa agctgcggat gaactccaga atggaggcaa ttagcataca
     3001 aatcaccaga atagtgatga tgtgtaattg cggaccccat ttgccgtcaa aaatcagcac
     3061 tccgatgatc ggcgtgaacg gcaaaatggc ataaaacgca ggggcagtgg tggtgatttc
     3121 actgacatct aacatttcat gagagatgtg ctctttttta tccagataac gttgccagaa
     3181 gaagtgggcg atcgccatgc cgataattgc agcaattgag ataggcagcg ttgttttgaa
     3241 ggcgaagtca atcagcgaca tttcggaagc ctgcgctgcc agcaccacat cccctgaagt
     3301 cggtgcgaga ataatcgccg ccggggaggc acaaatggca gcagctgcgc cacgactgat
     3361 accaacgttt accatcaccg gaaacagggt tgccatcagc aaaacaccca gaccggttgc
     3421 ggaagagacg gccagtgaca tcagacaggc aacaaaatag gcggcaatca tcagaaggta
     3481 aggggagtta atatactgca atggttttga cgccagcttg accaccatat cattcgcgcc
     3541 gatatgggtc atgtaagcgg caaagccaca cagcatcata atcatcatgc cgaggtcgcc
     3601 gccgcggctc attagcaata ttttaacgta ttcaacgata tccgtggcgc tgtagcctgt
     3661 tgaagcctgg ctggacggta acactttgtg ccccataatg gcactgataa tcagcaataa
     3721 caggccaccg acaaataaca cgccagtggc agaataccct ttaatgatgt agcgagctac
     3781 acccacaata accacaaccc caataaggag ttcaatgaat gtcagcataa tttttcctgt
     3841 ctccaggccc caaagtaaat aataaaaaat tcctaaaagt taaggaaaaa atatgcccaa
     3901 taaattggcg gtgaatgctg attaaaatca agaaaaactg ccattaagac attgaagttg
     3961 ctgtttttat acgtagataa caaaacgcat atttagcggt tggcaaatat cacataataa
     4021 tgtgtgtaaa ttgatggcgg attgatgcaa atttgttaat tgtaaggtgt tagctgcggt
     4081 tgtgtgctca aaaaataatc ttcc
//
LOCUS       DAHHTG010000230         4087 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.234, whole genome shotgun sequence.
ACCESSION   DAHHTG010000230 DAHHTG010000000
VERSION     DAHHTG010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4087)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4087)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4087
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.234"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            complement(<1..787)
                     /locus_tag="NBI74_004462"
     CDS             complement(<1..787)
                     /locus_tag="NBI74_004462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HCD1934682.1"
                     /translation="MFLVSLLRRIAFSYYDYKTYNFNIEKTDFVVIHIPDQIGDAMAI
                     FPVIRALELHKIKHLLIVTSTINLEVFNALKLEQTKLTLVTMTMQDHATLKEIKDLAK
                     NITLQYGTPDLCIEAMRKKNLKTMIFISQLKAKTNFQVVGLTMKCYSPLCKNASRMDQ
                     NLRAPVPMTWAFMMREAGFPAVRSIYELPLSDDVLDEVREEMRSLGSYIALNLEGSSQ
                     ERTFSLSIAENLIAKIQSETDIPIVIVYGPKGEDKARALVDCYN"
     gene            1102..2418
                     /gene="shiA"
                     /locus_tag="NBI74_004463"
     CDS             1102..2418
                     /gene="shiA"
                     /locus_tag="NBI74_004463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate transporter"
                     /protein_id="HCD1934683.1"
                     /translation="MDSTLISTRPDEGTLSLSRARRAALGSFAGAVVDWYDFLLYGIT
                     AALVFNREFFPQVSPAMGTLAAFATFGVGFLFRPLGGVIFGHFGDRLGRKRMLMLTVW
                     MMGIATALIGILPSFSTIGWWAPILLVTLRAIQGFAVGGEWGGAALLSVESAPKNKKA
                     FYSSGVQVGYGVGLLLSTGLVSLISMMTTDEQFLSWGWRIPFLFSIVLVLGALWVRNG
                     MEESAEFEQQQHNQAAAKKRIPVIEALLRHPGAFLKIIALRLCELLTMYIVTAFALNY
                     STQNMGLPRELFLNIGLLVGGLSCLTIPCFAWLADRFGRRRVYITGALIGTLSAFPFF
                     MALEAQSIFWIVFFSIMLANIAHDMVVCVQQPMFTEMFGASYRYSGAGVGYQVASVVG
                     GGFTPFIAAALITYFAGNWHSVAIYLLAGCLISAMTALLMKDNQRA"
     gene            2520..3974
                     /gene="amn"
                     /locus_tag="NBI74_004464"
     CDS             2520..3974
                     /gene="amn"
                     /locus_tag="NBI74_004464"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HCD1934684.1"
                     /translation="MNNKGSGLTPAQALDKLDALYEQSVVALRNAIGKYITSGELPDE
                     NARKQGLFVYPSLTVTWDGSTTNPPKTRAFGRFTHAGSYTTTITRPTLFRSYLNEQLT
                     LLYQDYGAHISVQPSQHEIPYPYVIDGSELTLDRSMSAGLTRYFPTTELAQIGDETAD
                     GIYHPTEFSPLSHFDARRVDFSLARLRHYTGTPVEHFQPFVLFTNYTRYVDEFVRWGC
                     SQILDPDSPYIALSCAGGNWITAETEAPEEAISDLAWKKHQMPAWHLITADGQGITLV
                     NIGVGPSNAKTICDHLAVLRPDVWLMIGHCGGLRESQAIGDYVLAHAYLRDDHVLDAV
                     LPPDIPIPSIAEVQRALYDATKLVSGRPGEEVKQRLRTGTVVTTDDRNWELRYSASAL
                     RFNLSRAVAIDMESATIAAQGYRFRVPYGTLLCVSDKPLHGEIKLPGQANRFYEGAIS
                     EHLQIGIRAIDLLRAEGDRLHSRKLRTFNEPPFR"
BASE COUNT          989 a          969 c          932 g         1197 t
ORIGIN      
        1 tattataaca atccactaat gccctggctt tatcttcccc tttgggtcca taaacgatca
       61 ctattggaat atctgtttca ctttgaattt ttgctattag attttctgca atcgataatg
      121 aaaatgtacg ttcctgcgag ctaccttcta aattgagcgc aatgtaagat cctaacgatc
      181 gcatttcctc gcgtacctca tcgagtacat catcgcttaa tggcaattca tatattgacc
      241 tgactgccgg aaaacccgcc tcacgcatca taaatgccca tgtcatgggt acaggagccc
      301 ggagattctg atccatccgg gacgcgttct tacacaaagg ggaatagcac ttcatggtta
      361 aaccgacgac ctggaaattc gtttttgctt tcaactgact gataaatatc atcgttttca
      421 ggttcttttt acgcatcgcc tctatacaaa gatccggcgt accatattgc agtgttatgt
      481 tcttcgctaa atcttttatt tcttttaatg ttgcgtgatc ctgcatagtc attgtgacta
      541 atgttaattt agtctgttca agtttgagcg cattaaagac ttctaaatta attgtcgacg
      601 taacaattaa tagatgctta attttatgca attcaagcgc ccgaataaca ggaaagatgg
      661 ccatagcatc gccaatctga tcgggaatat gtatgacaac aaagtctgtt ttttcaatat
      721 taaaattata agttttataa tcgtagtaac taaatgcaat acgtctcaac aatgatacta
      781 aaaacataac taacctcgcc tccctactgg ttataatgca atgcagtcta tcagaatcat
      841 caggttgcaa ttttgtgcat atgcggactt ttatgtttca tatctcgaac ctgtgggccc
      901 tctgcttaat ccttaaacaa caccagcaac tcctgcgctt tcatcttcca tcgaattttt
      961 catgttgccg ctaatcagcc ataaaatcat ttgcagatgc gctctgtcga ggtagtctca
     1021 taaggttcgt ttatagatcg acggcaatgt gagttacctt ttccatacta attataaaaa
     1081 gacagtacaa acaggatcat tatggactcc acgctcatct ccactcgtcc cgatgaaggg
     1141 acgctttcgt taagtcgcgc ccgacgagct gcgttaggca gtttcgctgg tgccgtcgtc
     1201 gactggtatg attttttact ctatggcatt accgccgcac tggtgtttaa tcgcgagttt
     1261 ttcccgcaag taagcccggc gatgggaacg ctcgccgcat ttgccacctt tggtgtcggc
     1321 ttccttttcc gaccgctcgg cggtgtcatt ttcggtcact ttggtgaccg gctgggacgt
     1381 aagcgcatgt taatgctgac cgtctggatg atgggcatcg cgacagcctt gattggtatt
     1441 cttccttcat tctcgaccat tgggtggtgg gcacctattt tgctggtgac actgcgtgcc
     1501 attcagggat ttgccgtcgg cggcgaatgg ggaggcgcag cgttgctttc cgttgaaagt
     1561 gcgccgaaaa ataaaaaagc cttttacagt agcggtgtac aagttggcta cggagtaggt
     1621 ttactgcttt caaccggact ggtttcattg atcagtatga tgacgactga cgaacagttt
     1681 ttaagctggg gctggcgcat tcctttcctg tttagcatcg tactggtact gggagcattg
     1741 tgggtgcgca atggcatgga ggagtccgcg gaatttgaac aacagcaaca taatcaagcg
     1801 gccgcgaaaa aacgcatccc ggttatcgaa gctctgttac gacatcccgg tgctttcctg
     1861 aagattattg cactacgact gtgcgagttg ctgacgatgt acattgttac tgcctttgca
     1921 cttaattatt caacccagaa tatggggtta ccgcgcgaac ttttccttaa tattggtttg
     1981 ctggtaggtg gattaagctg cctgacaatt ccctgttttg cctggcttgc cgatcgtttt
     2041 ggtcggcgca gggtttatat cacaggcgcg ttgatcggaa cgttgagcgc atttcctttc
     2101 tttatggcgc ttgaagcaca atctattttc tggatagttt tcttctccat aatgctggca
     2161 aacattgcgc atgacatggt ggtgtgtgtg caacaaccga tgtttaccga aatgtttggt
     2221 gccagttatc gctacagtgg tgctggcgtc ggttatcagg ttgccagtgt ggttggcggt
     2281 ggatttacac cttttattgc cgctgcactc atcacttact ttgccgggaa ctggcatagc
     2341 gtcgctattt atttgctggc tggatgcctg atttccgcaa tgaccgcttt gttgatgaaa
     2401 gacaatcaac gcgcttgata gcctggcgaa gatcatccga tcttcgcctt acacttttgt
     2461 ttcacattcc tgtgacatac tatcggatgt gcggtaattg tatggaacag gagacacaca
     2521 tgaataataa gggctccggt ctgaccccag ctcaggcact ggataaactc gacgcgctgt
     2581 atgagcaatc tgtagttgct ttacgcaacg ccattggcaa atatattaca agtggcgaat
     2641 tacctgatga aaacgcccgc aaacaaggtc tttttgtcta tccatcactg accgtaacct
     2701 gggacggtag cacaactaat ccccccaaaa cgcgcgcatt tggtcgtttt actcacgcag
     2761 gcagttacac caccacgatt actcgcccta ctctctttcg ttcgtatctc aatgaacaac
     2821 ttacgttgct gtatcaggat tatggtgcgc atatctctgt gcaaccctcg cagcatgaaa
     2881 tcccttatcc ttatgtcatc gatggctctg aattaacact tgatcgctca atgagcgctg
     2941 gtttaactcg ctacttcccg acaacagaac tggcgcaaat cggcgatgaa actgcagacg
     3001 gcatttatca tccaacggaa ttctccccgt tatcgcattt tgatgctcgc cgggttgatt
     3061 tttccctcgc acggttgcgc cattataccg gtacgccagt agaacatttt caaccgttcg
     3121 tcttgtttac caactacaca cgttatgtgg atgaattcgt tcgctgggga tgcagccaga
     3181 tcctcgatcc tgatagtccc tacattgccc tttcttgtgc tggcgggaac tggatcactg
     3241 ccgaaaccga agcgccagaa gaagccattt ccgaccttgc atggaaaaaa caccagatgc
     3301 cagcatggca tttaattacc gctgatggtc agggtattac tctggtgaat attggcgtgg
     3361 gaccgtccaa tgctaaaacc atctgcgatc atctggcagt gctacgcccg gatgtctggt
     3421 tgatgattgg tcactgtggc ggattacgtg aaagccaggc cattggagat tatgtccttg
     3481 cacacgctta tttacgcgat gaccacgttc ttgatgcagt tctcccgccc gatattccta
     3541 ttccgagcat tgcggaagtg caacgtgcgc tttatgacgc taccaagtta gtcagcggca
     3601 ggcctggtga agaagtcaaa cagcggttac gtactggcac ggtggtaacc acagatgaca
     3661 ggaactggga gttacgttac tcagcttctg cacttcgttt taacttaagc cgggccgtag
     3721 caattgatat ggaaagtgca accattgctg cgcaaggata tcgtttccgc gtgccatacg
     3781 ggacactact gtgtgtttca gataaaccgt tacatggtga gattaaactt cccggccagg
     3841 ctaaccgttt ttatgaaggc gctatttccg aacacctgca aattggcatt cgggcgatcg
     3901 atttgctgcg cgcagaaggc gaccgactgc attcacgtaa attacgaacc tttaatgagc
     3961 cgccgttccg ataataagtt atgaaccttc ttatcagaaa tagatgtgaa ggattaagta
     4021 agacatctgg caaatagcct gcaataaacg ctggctgttt gcttccttgg gcagatgcga
     4081 gttttat
//
LOCUS       DAHHTG010000231         4076 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.235, whole genome shotgun sequence.
ACCESSION   DAHHTG010000231 DAHHTG010000000
VERSION     DAHHTG010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4076)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4076)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4076
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.235"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            complement(<1..360)
                     /locus_tag="NBI74_004465"
     CDS             complement(<1..360)
                     /locus_tag="NBI74_004465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HCD1934685.1"
                     /translation="MRDIQMVLERWGAWAANNHEDVTWSSVAAGFKGLIPSKVKSRPQ
                     CCDDDAMIICGCMARLKKNNSDLHDLLVDYYVCGMTFMSLASKHCCSDGYIGKRLQKA
                     EGIIEGMLMALDIRLDMD"
     gene            complement(446..586)
                     /locus_tag="NBI74_004466"
     CDS             complement(446..586)
                     /locus_tag="NBI74_004466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcG family protein"
                     /protein_id="HCD1934686.1"
                     /translation="MMFESYMAERLRRRWVRLRLYRFPGSVLTDYRILKNYAKTLTGA
                     GV"
     gene            complement(583..945)
                     /gene="rusA"
                     /locus_tag="NBI74_004467"
     CDS             complement(583..945)
                     /gene="rusA"
                     /locus_tag="NBI74_004467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RusA"
                     /protein_id="HCD1934687.1"
                     /translation="MNTYSITLPWPPSNNRYYRHNRGRTHISAEGQAYRDNVARIIKN
                     AMLDIGLAIPVKISIECHMPDRRRRDLDNLQKAAFDALTKAGFWLDDAQVVDYRVVKM
                     PVTKGGKLELTITELGDE"
     gene            complement(942..1232)
                     /locus_tag="NBI74_004468"
     CDS             complement(942..1232)
                     /locus_tag="NBI74_004468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1364 domain-containing protein"
                     /protein_id="HCD1934688.1"
                     /translation="MADLRKAARGRECQVRIPGVCNGNPETSVLAHIRLAGLCCTGIK
                     PPDLIATIACSACHDEIDRRTHFVDAGYAKECALEGMARTQVIWLKEGVIEA"
     gene            complement(1225..1395)
                     /locus_tag="NBI74_004469"
     CDS             complement(1225..1395)
                     /locus_tag="NBI74_004469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1934689.1"
                     /translation="MATPLIRVMNGHIYKVPNRRKRKPELKPSEIPTLLGYTASLVDK
                     KWLRLAARRNHG"
     gene            complement(1395..1850)
                     /gene="ybcN"
                     /locus_tag="NBI74_004470"
     CDS             complement(1395..1850)
                     /gene="ybcN"
                     /locus_tag="NBI74_004470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA base-flipping protein YbcN"
                     /protein_id="HCD1934690.1"
                     /translation="MNLPQDGIKLHRGNFIAIGQQIQPYLEDGKCFRMVLKPWREKRS
                     LSQNALSHMWYSEISEYLISRGKSFATAAWVKDALKHTYLGYETKDLVDVVTGEITTI
                     QSLRHTSDLDTGEMYVFLCKVEAWAMNIGCHLTIPQSCEFQLLRDKQEA"
     gene            complement(1847..1948)
                     /locus_tag="NBI74_004471"
     CDS             complement(1847..1948)
                     /locus_tag="NBI74_004471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1934691.1"
                     /translation="MRTHNPNSLLPSQMQKCTCVFLHPASDLCGGEA"
     gene            2298..3341
                     /locus_tag="NBI74_004472"
     CDS             2298..3341
                     /locus_tag="NBI74_004472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCD1934692.1"
                     /translation="MKINKILSSATLLYGMSMAMSVGSCATPVQTNLPGYTPGADIIS
                     VSPTRNQVDLIGDVVYSQIKGTRSVRQLHMSVLVPRTNDLKPAIIYYPGGGFMSSEHD
                     KFIEMRMALAEAGFVVAAVEYRTIPDTFPAPVEDGKAAIRYLREHASNYGIDPQRIGV
                     LGDSAGGWLAQMMGTTNGDKTFDKGDFLQQSSDVQAVATLYGISDLLNIGEGFPESVQ
                     EVHRSPAVTEALMINGPAFRNFAGAPITASKEKALNASPIGHMKGVKPPFLIMHGSKD
                     TLVSPEQSAKLFRMLKKNGDNAEYVLVEGAEHGDNTWYQPIIINRVVEWFTKNLGTPI
                     KTATQQQKTNADL"
     gene            complement(3691..>4076)
                     /locus_tag="NBI74_004473"
     CDS             complement(3691..>4076)
                     /locus_tag="NBI74_004473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCD1934693.1"
                     /translation="IELQMKINNLIVALSVAPEVTAIAEKIRLLDKELRRASVSLKTL
                     KSKGVSSLGDFHAIDLTSKNGRELCRTLAYKIFEKIIINTDNKTCDIYFMNGIVFKHY
                     PLMKVISAQQAISTLKYMVDGEVYF"
BASE COUNT         1143 a          955 c          872 g         1106 t
ORIGIN      
        1 atccatatct aaccggatat ctaatgccat taacatccct tcaattatgc cctcagcctt
       61 ctgtaacctt ttcccgatat aaccatccga gcagcaatgc ttacttgcca gtgacatgaa
      121 tgtcatacca catacataat aatctactaa taaatcgtgt aaatcgctgt tgttcttttt
      181 cagacgggcc atgcacccgc aaatgatcat cgcgtcatcg tcacaacatt gcgggcgaga
      241 ttttactttt gaaggaatta atcccttaaa accggcggca acggacgacc aggtcacatc
      301 ttcatgatta ttagccgccc acgctcccca acgctcaaga accatctgaa tatcacgcat
      361 caacttactc cacaaaaatc agaccagaac gccaattaca agcaaaaatc aacaaaacag
      421 tattagttga ttgttatctc tgacttcata ctcctgctcc tgtcagggtt ttggcgtaat
      481 tcttcagtat tcggtaatcg gtcaaaacag aaccaggaaa acgatataag cgcaggcgca
      541 cccagcggcg gcgaagacgc tctgccatat aagactcaaa catcattcat ctcccagttc
      601 agtgatagtc agctccagct tcccaccttt ggtaacgggc atcttcacaa cgcggtaatc
      661 aacgacctga gcatcatcca gccagaaacc tgctttggtg agtgcgtcaa aagcggcctt
      721 ttgcagatta tcaaggtcac ggcgacggcg atccggcatg tggcactcaa tgctgatttt
      781 cacaggaata gccaggccga tatccagcat tgcgttttta atgattcggg cgacattatc
      841 gcggtatgcc tgcccctctg cgctgatatg cgtgcgtccg cgattatggc ggtaatagcg
      901 attattgctc ggcggccagg gtaatgtgat gctgtaggta ttcacgcctc aataaccccc
      961 tctttcaacc agataacctg tgttctcgcc ataccttcca gcgcgcattc ttttgcatat
     1021 ccagcgtcaa caaaatgcgt gcgacggtcg atttcgtcgt ggcaggcaga acatgcaatg
     1081 gtggcaatca ggtctggcgg tttgataccg gtacagcaca atccagccag ccggatatgt
     1141 gccagtactg acgtttcagg attgccatta catacgccag ggattcttac ctggcattcc
     1201 cgaccacgcg ctgcttttct caaatcagcc atgattcctc cttgctgcca gtcgcaacca
     1261 ttttttatca accaggctgg cggtataccc gagcagtgtt ggtatttcgg atggcttcag
     1321 ctcaggttta cgcttacgac gatttggtac tttgtagatg tgtccgttca tgacacgaat
     1381 aagcggtgta gccattacgc ctcctgcttg tcgcgcagca gctggaactc gcagctctgc
     1441 ggaatagtca ggtggcagcc aatattcatc gcccaggctt caaccttaca caggaagaca
     1501 tacatctctc cggtatcaag atcggaggta tggcgtaacg actggatagt agtgatttcg
     1561 ccggttacga catcaaccag gtccttggtt tcataaccga ggtatgtgtg tttgagagca
     1621 tcttttaccc atgctgcggt agcgaacgat ttccccctgc tgatgaggta ttcactgatt
     1681 tcgctgtacc acatatggct gagtgcattc tgggaaagac tgcgtttctc acgccacggt
     1741 ttaagcacca tgcgaaagca ttttccgtcc tccagataag gctggatctg ctggccgata
     1801 gcgatgaagt taccgcgatg cagtttgatg ccatcttgtg gtaggttcac gcttcacctc
     1861 cacagaggtc agacgctgga tgcaaaaaaa cgcaggtgca tttctgcatc tgtgaaggga
     1921 gaagagagtt tggattgtgt gtgcgcataa acgtccccgt ttagcgcaga agtcaccgga
     1981 gttgttcagg ctccggtgat acaattatgg cgaattgatt attcataatc aaacaagata
     2041 aggtctcaaa cttcatgcaa gccaagattt atttctgaca gaattataca aagaagctat
     2101 tggtcagaat ctactcggac tgtaaaacat acgcataacc ttaagctctc actttaagca
     2161 ttgttgaaat aatagccgtc aagtacaacc ttaaccacga ctgggatatt tccctggcta
     2221 ccacgagttg tacggctatt aaactgccgt taaattcagt aagagaattt catccgataa
     2281 gtcaaggcat gtaaaacatg aaaattaaca agatattatc atctgcaaca ctattgtatg
     2341 gtatgtcaat ggccatgtcg gtcgggagtt gtgcaacacc tgtccagact aatcttcctg
     2401 gttacacccc gggtgcagat atcattagtg tttcaccgac cagaaaccag gtcgatctca
     2461 ttggtgatgt tgtttattcc cagataaaag gaactcgttc tgtcagacag cttcacatgt
     2521 cagttcttgt cccgcgaaca aatgatttaa aaccagccat tatttattat cccggcggcg
     2581 gattcatgtc ttctgaacat gacaaattta ttgaaatgag aatggctctg gcagaagctg
     2641 gttttgttgt ggccgctgta gaatacagaa caattcctga tacatttcca gcaccagttg
     2701 aggatggaaa agctgcaata cgttacctga gagagcatgc cagcaattat gggattgatc
     2761 ctcaaagaat cggagttctg ggtgactctg ccggtggatg gcttgcccag atgatgggaa
     2821 ctacaaatgg tgacaaaacc tttgataaag gtgactttct tcagcaatcc tcagatgttc
     2881 aggcagttgc cacactttat gggatttctg acttgttgaa tattggcgag gggttccctg
     2941 aatcagtgca ggaggttcat cgctctcctg ccgtaaccga agccttaatg atcaatggcc
     3001 ctgcattcag aaattttgcg ggagccccca tcacagcgtc aaaagaaaaa gcgctaaacg
     3061 ccagtccaat cggacatatg aaaggagtaa aacccccatt tcttattatg catggtagca
     3121 aagacactct ggtttcacct gagcaaagcg ccaaactatt caggatgttg aagaagaacg
     3181 gtgataacgc tgagtacgtg ctggtagaag gggccgagca tggcgataat acatggtatc
     3241 agccaattat tataaacaga gttgttgagt ggtttactaa aaacctggga acccccataa
     3301 aaaccgctac ccaacaacaa aaaacaaacg ctgacctgta aaaagcagcc cgcacttatg
     3361 cgggctaacc tatttcactc gattcaaaag aaattatttt taatgggggc gtgaccagcc
     3421 tctcggtgct cagatactta atgaagtgga gtggcattct ggcaagaggt taacatcacc
     3481 tgttcgccat aaccaagcac gtgcacaaag tttgttatca gtgaattgtt ttgtgattgg
     3541 gtattgttga gctactagag caagaatgcc aacatccagt gacagtgcct atagtaaaac
     3601 tatagctcag ggcgcttcgt tcaagatagt taatatcata ttagttactt tactttcata
     3661 ccatagcacg gttgaaaaag tgattattac tcaaaaataa acctcaccat caaccatata
     3721 tttaagagta cttatcgcct gctgggcgga tattactttc attaaaggat agtgtttaaa
     3781 aacaatgcca ttcataaaat agatatcaca ggttttatta tctgtattaa ttataatttt
     3841 ttcgaatatt ttataggcaa gtgtacgaca tagctctcgc ccatttttac tggttaaatc
     3901 aatagcatga aaatcaccaa gtgaactcac ccctttactc ttcaaagttt ttaatgatac
     3961 cgaagccctt cgtaattcct tatctaatag tcttattttc tctgctatag cggtaacttc
     4021 aggcgcgaca gacaatgcaa cgattaaatt attaattttc atctgaagct caataa
//
LOCUS       DAHHTG010000232         4059 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.236, whole genome shotgun sequence.
ACCESSION   DAHHTG010000232 DAHHTG010000000
VERSION     DAHHTG010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4059)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4059)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4059
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.236"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            complement(34..362)
                     /locus_tag="NBI74_004474"
                     /pseudo
     CDS             complement(34..362)
                     /locus_tag="NBI74_004474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415918.1"
                     /note="involved in the synthesis of a cofactor required
                     for carnitine dehydratase and carnitine racemase
                     activities; frameshifted; incomplete; partial in the
                     middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetic acid degradation protein PaaY"
     gene            370..612
                     /locus_tag="NBI74_004475"
                     /pseudo
     CDS             370..612
                     /locus_tag="NBI74_004475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001067515.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            complement(784..1110)
                     /locus_tag="NBI74_004476"
     CDS             complement(784..1110)
                     /locus_tag="NBI74_004476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbL family protein"
                     /protein_id="HCD1934694.1"
                     /translation="MKRTLLLCAFLVGLVSSNVMALTLDEARTQGRVGETFYGYLVAL
                     KTDAETEKLVTDINAERKASYQQLAKQNNVSVDDIAKLAGQKLVERAKPGEYVQGING
                     KWVRKF"
     gene            complement(1118..1303)
                     /locus_tag="NBI74_004477"
     CDS             complement(1118..1303)
                     /locus_tag="NBI74_004477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnbE family lipoprotein"
                     /protein_id="HCD1934695.1"
                     /translation="MKILLAALTSSFMLVGCTPRIEVAAPKEPITINMNVKIEHEIII
                     KADKDVEELLETRSDLF"
     gene            complement(1300..3939)
                     /locus_tag="NBI74_004478"
     CDS             complement(1300..3939)
                     /locus_tag="NBI74_004478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbH family protein"
                     /protein_id="HCD1934696.1"
                     /translation="MLGKYKAVLALLLLIILVPLTLLMTLGLWVPTLAGIWLPLGTRI
                     ALDESPRITRKGLIIPDLRYLVGDCQLAHITNASLSHPSRWLLNVGTVELDSACLAKL
                     PQTEQSPAAPKTLAQWQAMLPNTWINIDKLIFSPWQEWQGKLSLALTSDIQQLRYQGE
                     KVKFQGQLKGQQLTVSELDVVAFENQPPVKLVGEFTMPLVPDGLPVSGHATATLNLPQ
                     EPSLVDAELDWQENSGQLIVLARDNGDPLLDLPWQITRQQLTVSDGRWSWPYAGFPLS
                     GRLGVKVDNWQAGLENALVSGRLSVLTQGQAGKGNAVLNFGPGKLSMDNSQLPLQLTG
                     EAKQADLILYARLPAQLSGSLSDPTLTFEPGALLRSKGRVIDSLDIDEIRWPLAGVKV
                     TQRGVDGRLQAILQAHENELGDFVLHMDGLANDFLPDAGRWQWRYWGKGSFTPMNATW
                     DVAGKGEWHDSTITLTDLSTGFDQLQYGTMTVEKPRLILDKPVVWGRDAQHPSFSGAL
                     SLDAGQTLFTGGSVLPPSTLKFSVDGRDPTYFLFKGDLHAGEIGPVRVNGRWDGIRLR
                     GNAWWPKQSLTVFQPLAPPDWKMNLRDGELYAQVAFSAAPEQGFRAGGHGVLKGGSAW
                     MPDNQVNGVDFVLPFRFADGAWHLGTRGPVTLRIAEVINLVTAKNITADLQGRYPWTE
                     EEPLLLTDVSVDVLGGNVLMKQLRMPQHDPALLRLNNLSSSELVSAVNPKQFAMSGAF
                     SGALPLWLNNEKWIVKDGWLANSGPMTLRLDKDTADAVVKDNMTAGSAINWLRYMEIS
                     RSSTKINLDNLGLLTMQANITGTSRVDGKSGTVNLNYHHEENIFTLWRSLRFGDNLQA
                     WLEQNARLPGNDCPQGKECEDKQ"
BASE COUNT         1006 a         1079 c          913 g         1061 t
ORIGIN      
        1 ttataattaa agggaaaaat aattatataa ctgttactgt ttcggtcgca gattctcatc
       61 aaacaccagg cgtttcctgc caggttcaac ttcccgcaat ggctcgactt gatgcaacgt
      121 ctgcttacaa cgagtcacca gcacctgatg ccctgctttc tcaatgccag ctcctgctcg
      181 ctgagttcac gaatcgcttt cgccgggctg ctgacaatca agtaattagt cggcatttct
      241 gctttggctt tcacaaatgc ggaaacacca acaatactgt tctcaccaat caccacaccg
      301 tccatcacta ccgcgttaat tcccactaat gtatggcggt ggttaatgca gccgtgaaga
      361 atagtcggag atacaggtat gtcagtacgt agtttgtacc gaatgtttgc cgataaaggt
      421 ttggtggtcg cgcaatatat tcgtaaccgt cgtctcgatt tttgtgcaga tgcgattcgc
      481 catgccgcag atgatgaaaa actggcaggc attggctttc attggggatt ttccgaccag
      541 agtcattttt caacggtatt taaacaacgc tttgggatga cgccaggcga gaatcggcgt
      601 aaattccgct aactaattcg cgttgcatgt acagcaacgc gaattagtta aaacagatat
      661 ttgactatgc atttttcagg gcgaaaggca atttgacagg cgtttttgaa taatcatctt
      721 gtttccggaa aatagattaa tcgccttctc ctgtagcgtt atcccgatat tgcggcttta
      781 ctattaaaat tttcgcaccc atttaccgtt tatcccttgt acatattccc cgggtttggc
      841 ccgttctacg agcttttgcc cggcgagttt cgcgatatca tctaccgaca cattattttg
      901 ctttgccagt tgttgatagc tcgctttacg ttcggcatta atgtcggtta ctaatttctc
      961 agtttcagca tctgttttca gcgcaaccag ataaccataa aatgtttcgc ctacccgccc
     1021 ctgagttctg gcttcatcca gagtcaatgc cattacattg ctgcttacca agccaacaag
     1081 aaatgcacaa agaagtaatg ttcttttcat catcacctca aaaaagatcg ctacgggtct
     1141 caagcagctc ttcgacatct ttgtctgcct tgatgatgat ctcatgctca attttaacgt
     1201 tcatattgat agtgatcggt tccttaggtg cagcgacttc aatacgaggc gtacagccaa
     1261 ccagcataaa agatgacgtc aacgcagcca gtaaaatttt cattgtttat cctcacactc
     1321 ttttccttgc ggacagtcat ttcccggcag acgcgcgttc tgctccagcc atgcctggag
     1381 attatcgcca aagcgtaaac tgcgccacag cgtaaaaata ttctcttcat gatggtaatt
     1441 aagatttacc gtaccgcttt taccatcaac gcgactggta cctgtaatat tggcctgcat
     1501 ggttaataaa ccgagattat ctaaattaat ttttgtcgat gaacggctaa tttccatata
     1561 gcgcaaccag ttaattgctg aacccgcggt catattgtct ttcaccaccg catctgcggt
     1621 gtctttatcc agccgcaagg tcattggtcc gctatttgcc agccagccgt ctttcactat
     1681 ccatttttca ttgtttaacc ataacggcag tgcaccacta aatgccccgg acatggcgaa
     1741 ttgtttcgga ttaacggcgc taaccagttc gctggatgag agattattca gtcgcaacag
     1801 tgccgggtca tgttgcggca tacgtaattg tttcatcagc acgttaccgc ctaacacatc
     1861 gacactaaca tcagtcaaca gcaatggttc ttcttcagtc cacggataac gcccttgcaa
     1921 atcagccgta atatttttcg ctgtcaccag attaatcact tcggcaattc gcaacgtaac
     1981 ggggccgcga gtccccagat gccaggctcc atcggcaaaa cggaaaggca ggacaaaatc
     2041 gacaccgtta acctgattat ctggcatcca ggcactaccg cctttcaata cgccgtgccc
     2101 ccccgcgcgg aatccttgtt cgggcgctgc tgaaaatgcg acctgagcat acagttcacc
     2161 atcgcgtaag ttcatcttcc agtcgggtgg cgccaacggc tggaatacag tcagtgattg
     2221 tttaggccac caggcgttac cgcgcagacg aataccgtcc cagcgaccat ttacccgaac
     2281 cgggccaatc tcaccagcat gtaaatcgcc tttaaagagg aaatacgtcg gatcgcgccc
     2341 atcgacacta aattttaagg ttgatggcgg taacacactg ccgccagtga acagcgtttg
     2401 cccggcgtcc agtgacagag cgccgctgaa ggagggatgc tgtgcgtcac gtccccagac
     2461 gacgggcttg tcgagaatta atcgcggctt ttctaccgtc atcgtaccgt attgtaactg
     2521 gtcgaaaccg gtggagagat cggtcagcgt aatcgtgctg tcatgccact caccttttcc
     2581 tgcgacatcc caggtggcat tcatcggtgt aaaactccct tttccccagt agcgccactg
     2641 ccagcggcca gcgtcaggga gaaaatcatt cgccagccca tccatatgca gcacgaaatc
     2701 gcccagttca ttttcatgcg cctgcaaaat ggcctgcaaa cgtccgtcaa caccacgttg
     2761 ggtgactttt acacccgcta aaggccagcg gatttcatcg atgtccagcg aatcaatgac
     2821 tcttcccttt gaacgaagta acgcgccagg ctcaaaggtc agcgttgggt cagacagact
     2881 tccacttagc tgcgcaggta aacgggcata taaaatgagg tccgcctgtt tcgcttcacc
     2941 ggtcagctgc agaggcagct ggctgttatc catgcttaat tttcctgggc caaaatttaa
     3001 caccgcgttg cctttacccg cttgcccctg ggtcagcaca ctcagtcgcc cgctgaccag
     3061 agcgttctcg agccctgcct gccagttgtc gactttgaca cccagtcggc cactcaaagg
     3121 aaaacctgca tacggccagc tccagcgacc atcgcttacg gtcaattgtt gacgagtaat
     3181 ttgccacggc aaatcgagca acggatcgcc gttatcccgt gccagcacaa tcaattgccc
     3241 gctattttcc tgccagtcca gctcggcatc caccagtgaa ggctcctgcg gtaagtttaa
     3301 cgtcgcagta gcatgaccac ttacaggaag tccatccggc acgagcggca tagtaaattc
     3361 ccccaccagt tttaccggcg gctgattttc aaacgcgacg acatccagtt cgctgactgt
     3421 aagttgttgc cctttaagct ggccttgaaa tttaactttt tcgccctgat aacgcagttg
     3481 ctggatatcg gaggttaatg cgagagagag ttttccctgc cattcctgcc agggagaaaa
     3541 aatcagttta tcgatattga tccaggtgtt aggcaacatg gcctgccact gcgcgagggt
     3601 tttgggagcg gctggcgatt gctccgtctg cggcaatttc gccagacaag cagaatcaag
     3661 ttctaccgtg ccgacgttca ataaccagcg gctgggatgt gaaaggctgg cgttggtgat
     3721 atgcgcaagc tgacaatctc ccaccagata acggagatcg gggatgatta aacctttacg
     3781 cgtaatgcgt gggctttcat ctaatgcaat acgtgtcccg agcggtagcc agatgcccgc
     3841 cagcgtggga acccacagcc cgagcgtcat cagcagcgtc aacggcacaa gaataatcag
     3901 taataacagc gcgagaacgg ctttatattt acccagcatg ggtagttaat atcctgattt
     3961 agcgaaaaat taagcattca atacgggtat tgtggcatgt ttaaccgttc agttgaaggt
     4021 tgcacctaca ctaagcatag ttgttgatga atttttcaa
//
LOCUS       DAHHTG010000233         3977 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.237, whole genome shotgun sequence.
ACCESSION   DAHHTG010000233 DAHHTG010000000
VERSION     DAHHTG010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3977)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3977)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3977
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.237"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            116..319
                     /locus_tag="NBI74_004479"
     CDS             116..319
                     /locus_tag="NBI74_004479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AaeX family protein"
                     /protein_id="HCD1934697.1"
                     /translation="MSLFPVIVVFGLSFPPIFFELLLSLAIFWLVRRVLVPTGIYDFV
                     WHPALFNTALYCCLFYLISRLFV"
     gene            327..1259
                     /gene="aaeA"
                     /locus_tag="NBI74_004480"
     CDS             327..1259
                     /gene="aaeA"
                     /locus_tag="NBI74_004480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeA"
                     /protein_id="HCD1934698.1"
                     /translation="MKTLIRKFSRTAITVVLVILAFIAIFNAWVYYTESPWTRDARFS
                     ADVVAIAPDVSGLITQVNVHDNQLVKKGQVLFTIDQPRYQKALEEAQADVAYYQVLAQ
                     EKRQEAGRRNRLGVQAMSREEIDQANNVLQTVLHQLAKAQATRDLAKLDLERTVIRAP
                     ADGWVTNLNVYTGEFITRGSTAVALVKQNSFYVLAYMEETKLEGVRPGYRAEITPLGS
                     NKVLKGTVDSVAAGVTNASSTRDDKGMATIDSNLEWVRLAQRVPVRIRLDNQQENIWP
                     AGTTATVVVTGKQDRDESQDSFFRKMAHRLREFG"
     gene            1265..3232
                     /gene="aaeB"
                     /locus_tag="NBI74_004481"
     CDS             1265..3232
                     /gene="aaeB"
                     /locus_tag="NBI74_004481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeB"
                     /protein_id="HCD1934699.1"
                     /translation="MGIFSIANQHIRFAVKLATAIVLALFVGFHFQLETPRWAVLTAA
                     IVAAGPAFAAGGEPYSGAIRYRGFLRIIGTFIGCIAGLVIIIAMIRAPLLMILVCCIW
                     AGFCTWISSLVRIENSYAWGLAGYTALIIVITIQPEPLLTPQFAVERCSEIVIGIVCA
                     IMADLLFSPRSIKQEVDRELESLLVAQYQLMQLCIKHGDGEVVDKAWGDLVRRTTALQ
                     GMRSNLNMESSRWARANRRLKAINTLSLTLITQSCETYLIQNTRPELITDTFREFFDT
                     PVETAQDVHKQLKRLRRVIAWTGERETPVTIYSWVAAATRYQLLKRGVISNTKINATE
                     EEILQGEPEVKVESAERHHAMVNFWRTTLSCILGTLFWLWTGWTSGSGAMVMIAVVTS
                     LAMRLPNPRMVAIDFIYGTLAALPLGLLYFLVIIPNTQQSMLLLCISLAVLGFFLGIE
                     VQKRRLGSMGALASTINIIVLDNPMTFHFSQFLDSALGQIVGCVLAFTVILLVRDKSR
                     DRTGRVLLNQFVSAAVSAMTTNVARRKENHLPALYQQLFLLMNKFPGDLPKFRLALTM
                     IIAHQRLRDAPIPVNEDLSAFHRQMRRTADHVISARSDDKRRRYFGQLLEELEIYQEK
                     LRIWQAPPQVTEPVHRLTGMLHKYQHALTDS"
     gene            3324..3596
                     /locus_tag="NBI74_004482"
     CDS             3324..3596
                     /locus_tag="NBI74_004482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="barstar family protein"
                     /protein_id="HCD1934700.1"
                     /translation="MNIYTFDFDEIESQEDFYRDFSQTFGLAKDKVRDLDSLWDVLMN
                     DVLPLPLEIEFVHLGEKTRRRFGALILLFDEAEEELEGHLRFNVRH"
     gene            complement(3651..3914)
                     /gene="yhcN"
                     /locus_tag="NBI74_004483"
     CDS             complement(3651..3914)
                     /gene="yhcN"
                     /locus_tag="NBI74_004483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid stress response protein YhcN"
                     /protein_id="HCD1934701.1"
                     /translation="MKIKTTVAALSVLSVLSFGAFAADSIDAAQAQNREAIGTVSVSG
                     VASSPMDMREMLNKKAEEKGATAYQITEARSGDTWHATAELYK"
BASE COUNT          919 a          962 c         1060 g         1036 t
ORIGIN      
        1 atattaaata tcaatctaca gcaatgttgc tctcgcccgg ctttccatgc ccttctctgc
       61 ggcgacagat gctgaaaata ataacgcctg ctctctctat acaaccaagg tcaacatgag
      121 tctgtttccc gttatcgtgg tgtttgggct gtccttccca ccgatatttt tcgaattgct
      181 tttatcactg gcgattttct ggctggtgcg ccgggtactt gtgccaacag gtatctacga
      241 ctttgtctgg catccggcgt tgttcaacac cgcgctctat tgctgcttgt tttatttgat
      301 atcgcgactg ttcgtttgag gttgaagtga aaacactaat aagaaaattc tcccgtacgg
      361 ccatcacggt cgtattagtc attctggcct tcatcgcaat ttttaatgcc tgggtctatt
      421 acaccgaatc cccgtggacg cgtgacgcgc gctttagcgc tgacgtcgtt gcgatcgcgc
      481 cggacgtttc tggactcatt acccaggtga atgttcatga taaccagctg gtgaaaaaag
      541 gacaggtact gttcaccatc gaccagccgc gctatcaaaa ggcgcttgag gaagcgcaag
      601 ccgatgttgc ttattatcag gtactggcac aggagaaacg ccaggaggcc ggacgtcgta
      661 accgtctcgg tgtgcaggcg atgtcccgcg aagagatcga ccaggccaac aacgtactac
      721 aaacggttct gcatcagtta gcgaaagcgc aggcgacccg cgatctggca aaactggatc
      781 ttgaacgcac ggtgatccgc gcgccagcag atggctgggt gaccaacctc aacgtctata
      841 ccggtgagtt tattacccgt ggatcaacgg cggttgcgct ggtgaaacag aactccttct
      901 atgtactggc ctatatggaa gaaactaagc tggaaggggt gcgccctggg tatcgtgcgg
      961 agatcacgcc gcttggcagt aacaaagtgc tgaaagggac tgttgatagt gttgccgcag
     1021 gggtcaccaa tgccagcagc acgcgtgacg acaaagggat ggcgaccatc gactctaacc
     1081 ttgaatgggt acgtttggcg caacgtgttc cggtacgtat tcgtctcgac aaccagcaag
     1141 agaacatctg gcctgcgggc accactgcta cggtggtagt cactggcaaa caagatcgcg
     1201 acgaaagtca ggattcgttc ttccgtaaaa tggctcatcg cctgcgtgag tttggttaat
     1261 cacgatgggt attttctcca ttgctaatca acatattcgc tttgcggtaa aactggcgac
     1321 cgccattgta ctggcgctgt ttgttggctt tcacttccag ctggaaacgc cacgctgggc
     1381 ggtactgaca gcggcgattg ttgccgccgg tccggccttt gctgcgggag gtgaaccgta
     1441 ttctggcgct attcgctatc gtggcttttt gcgcatcatc ggcacattta ttggctgtat
     1501 tgccggactg gtaatcatca ttgcgatgat ccgagcacca ttattgatga ttctggtgtg
     1561 ctgtatctgg gccggttttt gtacctggat atcctcgctg gtacgaatag aaaactcgta
     1621 tgcgtggggg ctggccggtt ataccgcgct gatcattgtg atcaccattc agccggaacc
     1681 attgcttacg ccgcagtttg ccgtcgaacg ttgtagcgag atcgttatcg gtattgtgtg
     1741 tgcaattatg gcggatttgc tcttttctcc gcgatcgatc aaacaagaag tggatcgaga
     1801 gctggaaagt ttgctggtcg cgcaatatca attaatgcaa ctctgtatca agcatggcga
     1861 tggtgaagtt gtcgataaag cctggggcga tctggtgcga cgtactacgg cgttacaagg
     1921 tatgcgcagc aacctgaata tggaatcttc ccgctgggcg cgggctaatc gacgtttaaa
     1981 agcgatcaat acgctatcgc tgacgctgat tacccaatcc tgcgaaactt atcttattca
     2041 gaatacgcgc ccggaattga tcactgatac tttccgcgaa ttttttgaca cgccggtaga
     2101 aactgcgcag gacgtccaca agcagctcaa acgcctgcgg agagtgatcg cctggaccgg
     2161 ggaacgggaa acgcctgtta ccatttatag ttgggtcgcg gcggcaacgc gttatcagct
     2221 tctcaagcgc ggcgttatca gtaacacaaa aatcaacgcc accgaagaag agatcctgca
     2281 aggcgaaccg gaagtaaaag tagagtcagc cgaacgtcat catgcaatgg ttaacttctg
     2341 gcgaaccaca ctttcttgca ttctgggcac gcttttctgg ctgtggacgg gctggacttc
     2401 cggcagtggt gcaatggtga tgattgcggt cgtgacgtca ctggcaatgc gtttgccgaa
     2461 tccacgcatg gtggcgatcg actttatcta cgggacgctg gcagcgctgc cgttagggct
     2521 gctctacttt ttggtgatta tccctaatac ccaacagagc atgttgctgc tgtgtattag
     2581 cctggcagtg ctgggatttt tcctcggtat agaagtacag aaacggagat tgggctcgat
     2641 gggggcgttg gccagcacca taaacattat cgtgctggat aacccgatga ctttccattt
     2701 cagtcagttt ctcgacagcg cattagggca aatcgtcggc tgtgtgctcg cgttcaccgt
     2761 tattttgctg gtgcgggata aatcgcgcga caggactgga cgtgtactgc ttaatcagtt
     2821 tgtttctgcc gctgtttccg cgatgactac caatgtggca cgtcgtaaag agaatcacct
     2881 cccggcactt tatcagcagc tgtttttgct gatgaataag ttcccagggg atttgccgaa
     2941 atttcgcctg gcgctgacga tgattatcgc gcaccagcgc ctgcgtgatg cgccgatccc
     3001 ggttaacgag gatttatcgg cgtttcaccg acaaatgcgc cgcacagcag accatgtgat
     3061 atctgcccgt agcgatgata aacgtcgtcg gtactttggc cagttgctgg aagaactgga
     3121 aatctaccag gaaaagctac gcatctggca agcgccaccg caggtgacgg aaccagtaca
     3181 tcggcttacg ggtatgctcc ataagtatca acatgcgttg accgatagtt aagtcaaaac
     3241 cgacgcctga agcgtcggtt ttttcatggc tatacttagc gatgaacggc agaactcgcc
     3301 gcgaaacgtg acggtggcaa cagatgaata tttatacctt tgattttgat gagattgaga
     3361 gtcaggagga tttttatcgt gactttagcc aaacctttgg tctggcgaaa gataaggtac
     3421 gcgatctcga ctcactatgg gatgtgttaa tgaacgatgt cctgccgcta ccacttgaga
     3481 ttgaatttgt tcatctggga gagaaaacgc gtcgccgttt tggcgcgtta atattgctgt
     3541 ttgatgaggc agaggaagag ctggaagggc atttgcgttt taatgttcgt cattagcgca
     3601 aaaaaagccc ccgaaccggg ggcaatatcg tcggacaaga cgatgagggt ttatttgtac
     3661 agttcagccg tagcgtgcca ggtgtcacca ctacgagctt cagtaatctg gtaggccgtt
     3721 gcgcctttct cttccgcttt tttgttcagc atttcacgca tatccattgg cgaagacgcc
     3781 acaccactta cggatacggt cccgattgct tcacgatttt gtgcttgtgc agcatcaatg
     3841 gagtcggcag cgaatgcacc gaaagagaga acagaaagta cgcttaatgc agcaacagtg
     3901 gttttgattt tcatgatttt tacctcgaca taatctttta gctgggcctt tgtttcgtga
     3961 cccatatcac aaaatca
//
LOCUS       DAHHTG010000234         3962 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.238, whole genome shotgun sequence.
ACCESSION   DAHHTG010000234 DAHHTG010000000
VERSION     DAHHTG010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3962)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3962)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3962
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.238"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            complement(258..578)
                     /locus_tag="NBI74_004484"
     CDS             complement(258..578)
                     /locus_tag="NBI74_004484"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1934702.1"
                     /translation="MTGTAAQTPVMDVMEIPAEPQVFQSGTQSTGLELVDTYLWIFKH
                     FIEGKELTRSLACQVYIKPYSDRMDSVTFQSVAKRSKVFLNKLQEPTSEMINKRGNTW
                     CWKS"
     gene            1565..2542
                     /locus_tag="NBI74_004485"
     CDS             1565..2542
                     /locus_tag="NBI74_004485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HCD1934703.1"
                     /translation="MDKSSGELVTLTPNNNNTVQPVALMRLGVFVPTLKSLKNSKKNT
                     LSRTDATEELTRLSLARAEGFDKVEITGPRLDMDNDFKTWVGIIHSFARHNVIGDKVE
                     LPFVEFAKLCGIPSSQSSRRLRERISPSLKRIAGTVISFSRTDEKHTREYITHLVQSA
                     YYDTERDIVQLQADPRLFELYQFDRKVLLQLKAINALKRRESAQALYTFIESLPRDPA
                     PISLARLRARLNLKSPVFSQNQTVRRAMEQLREIGYLDYTEIQRGRTKFFCIHYRRPR
                     LKAPNDESKENQLPPSPAEKISPEMAEKLALLEKLGITLDDLEKLFKSR"
     gene            complement(2785..3525)
                     /locus_tag="NBI74_004486"
     CDS             complement(2785..3525)
                     /locus_tag="NBI74_004486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCD1934704.1"
                     /translation="MNNVIPLQNSPERVSLLPIAPGVDFATALSLRRMATSTGATPAY
                     LLAPEVSALLFYMPDQRHHMLFATLWNTGMRIGEARMLTPESFDLNGVRPFVRILSEK
                     VRARRGRPPKDEVRLVPLTDISYVRQMESWMITTRPRRREPLWAVTDETMRNWLKQAV
                     RRAEADGVHFSIPVTPHTFRHSYIMHMLYHRQPRKVIQALAGHRDPRSMEVYTRVFAL
                     DMAATLAVPFTGDGRDAAEILRTLPPLK"
     gene            complement(3646..3834)
                     /locus_tag="NBI74_004487"
     CDS             complement(3646..3834)
                     /locus_tag="NBI74_004487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001309252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein kinase"
                     /protein_id="HCD1934705.1"
                     /translation="MASDTFSCRRRLSAGFFSVSACSWHYLNSPKNGKPALSPRHDWL
                     NTGSLLQNIPLRIWFYPQ"
BASE COUNT          997 a          979 c          969 g         1017 t
ORIGIN      
        1 tatatcgacc taacccttga tcagtttcca ggctatcata accggattgt ggcagagccc
       61 gttgagtccg gcggacagct cgcggcagac ctcaacaggg agcctgccat cagtacagct
      121 gatggaatag tggccagccc atcagataag gttaattaca ttactgaaca gaaaaatcgc
      181 cagccaatgg tgatcattac agaatgttac aagctgatca gaatgacggc aagatttata
      241 ccaatgtgcc agtcatctca actcttccag caccaggtat tccctcgctt atttatcatt
      301 tcagacgttg gctcctgcag tttgttcagg aacaccttag atcgtttagc tactgactgg
      361 aaggtgacac tgtccatcct gtcgctatag ggcttgatat aaacctggca ggcaagggat
      421 ctggttaact cctttccttc aataaagtgt ttgaagatcc agagatacgt atccacaagc
      481 tccagccctg tactctgtgt ccccgactgg aagacctgcg gttcagctgg tatttccatt
      541 acatccatga ccggtgtctg cgcagcagtg cctgtcatca ggatcctgtc actctttcga
      601 ccgacagatt atggcataca gttgggctcg taagatccag agtcaataac tgacagggtt
      661 tattctgtca tcctccggca gcaaaggcat atacgatatt cacttatttt gtcggtccaa
      721 cggcttttta cagtagctcg ttttgcccaa ctgatgctac agctctttgg tttgcttcat
      781 cacggatatc agctccgaca caggcacagc tgactggttg caacgtctgc acttctctgg
      841 acctgaaacg tcaattatca ggattctgac gattagtctt aagactgtcg gtaaattaag
      901 tgatgccagt tgtcaggata ttcatggagc cagtctgcat gcgcctctgg cgttgagaca
      961 aacatatgaa gtgtatttcg gatatgactg tacgctcagg aagtacggca gattccgtca
     1021 aaaattacct gtaaagacag acttcagaaa aaacagtgct cgccgcagac cggtgaccgg
     1081 gcgaagcccg cgaaccggcg aggaagcgca atatatctgt agttacagtg agcaggtacg
     1141 ccctgaagcc ttcataatcg gtagtgtatg ttgtttttcc ggaatgattt atccacatat
     1201 ccagaggccc gatccagtaa acagatccat gaatgatcaa caaaggatcc attaaagatc
     1261 cccatatcgc tgcaaacctt gtcactcatg ggccgggacc acgttcacat aagctgtggc
     1321 atgttatgga taaactgtaa caggttcatg ataagctgta ttcagtaatg catatactga
     1381 agtaagttaa tgacataaac tatggtcagt acgccagact cagttgttaa atacagactg
     1441 caggtttttc ttcagtcagt tgacggggtt ctgacacacg attttctgtt tattctttta
     1501 ctgtccacag gcaggaggct ttctggaaaa cgaaaattca gacatcaaaa aactgttcgg
     1561 cgaggtggat aagtcgtccg gtgagctggt gacactgaca ccaaacaata acaacaccgt
     1621 acaacctgtg gcgctgatgc gtctgggcgt ctttgtacct acccttaagt cactgaagaa
     1681 cagtaaaaaa aatacactgt cacgcaccga tgccacggaa gaactgacgc gtctttctct
     1741 ggcccgtgca gaaggattcg ataaggttga gatcaccggc ccccggctgg atatggataa
     1801 cgatttcaag acctgggtgg ggatcattca ttcctttgcc cgccataacg tgattggtga
     1861 caaagttgaa ctgccttttg tcgagtttgc aaaactgtgt ggtataccct caagccagtc
     1921 atcacgcagg ctgcgtgagc gcatcagccc ttccctgaaa cgcattgccg gtaccgtgat
     1981 ctccttttcc cgcaccgatg agaagcacac ccgggaatac atcacccatc tggtacagtc
     2041 agcctactac gatactgaac gggatattgt tcagttacag gctgatcccc gcctttttga
     2101 actgtaccag tttgacagaa aggtccttct tcagcttaag gcgattaatg ccctgaagcg
     2161 acgggagtcc gcccaggcac tttacacctt tatagagagc ctgccccggg atccggcacc
     2221 gatatcgctg gcgcggctgc gtgcacgcct caatctgaag tctcctgtat tttcccagaa
     2281 ccagacggtc agacgggcaa tggagcagtt gcgcgagatt ggatatcttg attacacgga
     2341 gatccagcgg gggcggacaa aattcttctg tattcactac cggcgtcccc ggttaaaagc
     2401 gccgaatgat gagagtaagg aaaatcagtt gccaccttca cctgcggaaa aaatcagtcc
     2461 ggagatggcg gagaagcttg ccctgcttga aaaactgggc atcacgctgg atgacctgga
     2521 aaaactcttc aaatcccgct gaacataaac tgtagtcaga taagagagac tctttactga
     2581 ctacagttta tattaacagg tgcagtgagt gatgtgctca ctgtagttta tattcagttt
     2641 cctgctgcac tgtctgtggc tgagctgcca tctgcctgtt ccttacataa aacacggctt
     2701 actcactaca gtttatctac ctgctatagc ttatgttatg cctgttctgc ttacctccgc
     2761 ttatataagg aagagcgtat gctgtcactt caggggaggc agtgtacgca ggatctctgc
     2821 agcatcgcgc ccgtcacctg tgaaaggcac tgccagcgtg gcagccatat ccagcgcaaa
     2881 aactctggta taaacctcca tcgaacgtgg atccctgtga ccagccagtg cctggatgac
     2941 tttccggggc tggcggtgat agagcatgtg catgatatag ctgtgccgga aggtatgtgg
     3001 tgtgaccgga atcgaaaaat gcacgccgtc agcttcggcc cgtctgacag cctgcttcag
     3061 ccagttgcgc atggtttcgt cggtcacggc ccataatggt tcacgacgac ggggccgggt
     3121 ggtgatcatc cagctttcca tctgcctgac atagcttata tctgtcagcg gcaccagtcg
     3181 cacttcatct tttggcgggc gtccgcgtcg cgcacgcact ttttcggaca ggatccgcac
     3241 aaacggtctt actccattca ggtcaaatga ttccggcgtc agcatccggg cttcgccaat
     3301 acgcattccg gtattccaga gggtggcgaa cagcatatgg tgacgctgat ccggcatata
     3361 gaagagaagg gcactcactt ccggggccag caggtaggcc ggtgtggccc ccgtggaagt
     3421 ggccattctt ctcagggaga gcgctgttgc aaaatccacc cccggcgcaa tgggtaacag
     3481 ggagacgcgt tctggtgaat tctgcagggg aatgacattg ttcatgagtt atttttcacg
     3541 tagcacacgc actgtttatg gatacaggta ttctgacctg aacgcccgga tccttccacg
     3601 acaataaatc gcagcgcgcg gatccttttg tttgcttatc acgcattatt gcgggtaaaa
     3661 ccaaatacgt aaaggtatgt tttgcagcag ggatcccgta ttaagccagt cgtggcgcgg
     3721 gctgagggct ggtttgccgt ttttgggaga attgaggtaa tgccaggaac aggcagatac
     3781 ggagaaaaat cctgcagata accttctacg acaactgaag gtgtctgacg ccatattctt
     3841 agctacttct cagttttaag gcggcttagt cacaacataa ggttatgcat aataagcata
     3901 ttttattatg ttatgttatg gtcttgttca tgcatgaact gttatctatt cagtcacttt
     3961 ta
//
LOCUS       DAHHTG010000235         3925 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.239, whole genome shotgun sequence.
ACCESSION   DAHHTG010000235 DAHHTG010000000
VERSION     DAHHTG010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3925)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3925)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3925
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.239"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            complement(<1..3701)
                     /locus_tag="NBI74_004488"
     CDS             complement(<1..3701)
                     /locus_tag="NBI74_004488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016158919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HCD1934706.1"
                     /translation="MNKIYMLKKNHRGELVAVSEVVKNIKRNSISTFLLKSKLSTLLL
                     ISIMVGGYSFPSCASVVSSTTSYQTYRDFAENKGEFRPGAEDVPLYDKNGNPVMTLNK
                     APMIDFSSNDSTGVATLVSPQYVVSVKHNGGYQYVKFGYADDSSYALVDRNNHWRDFH
                     TPRLNKIVTEVTPLDITNAGTAKGTYQNADRFPVFYRVGTGTQYVKDTNGKITYLMGA
                     YSYKTGGIVNTPAISDWSFVTNTTNTPLSTYGTPGDSGSPLFAWDADQNKWVLLAVLN
                     SYAGVNGNTNWYTIIPAGDVKNTMKQDADAPVNTKQGEGDIHWSYDEKTGLGSLTQGS
                     TSWEMHGNLGATWPASLNSGKDLTFQGGGTVVLENTVNQGAGTLTFDDDYIVKPVDTQ
                     TWKGGGIIVNGEHLVDWQINGVTGDSLHKLGTGTLKINGTGVNPGSLSVGDGTVILAQ
                     RADDNGLSQAFSSVSIVSGRPTLVLNDDKQINPDNIKWGYHGGKLDINGNSLTFHKLN
                     GADDGAILTNSGSMANVNLDFNSPDTTATVANIWHGHFTGNLNINNEVTAGTQNDFAI
                     DGGVNTQGSITQQNGRLFMQGHPVVHAVSSQDVANKLKALGDNSVLTQPVSFTQNDWE
                     NRQFSMAELNLQNAEFNLARNASLNTRINAEHSTVTLGSEDLYIDLNDGNGVATKPTL
                     GKSKATAEDDQSRFNGHVQLQQGSALTINEHFAGGIDSADSATTITSTDTTLNQLSRF
                     TQSSLSLGEGAKLTATAGLLSDGTVSSNAGASLSLLSDQPGTMYSAQSWELSGQDTSL
                     NVGAGGIITGDINANDAASISFGTTDINQSTNYYGNINAPLASVTMKDTAWQVNKQSV
                     AKSLTLNGSTLSFNRFGQGGLTSDTLEATNSSFIINADGKAADTVTVNQALTGGNNTL
                     VVIPTTNSVKQGGDPVSLVTAPKNTQSNIFTLNPVSINAGFHSFTPQLDVLETDVNKQ
                     WRLEGFYIQPDKAALRTGKSFMDLGYKNFITEINNLNDRMGDLRHTHGETGAWARLNS
                     GSGSATDGFTGSYTHLQIGADRKHIIEGGELFTGVTATFTSSNNRGTGWSGRTKSTGI
                     GVYASAMFDSGLYVDTIGKYVRHDNHYSSSALGMPEQDYGSHSWYLGAEAGWRFSLPD
                     ETYIQPQTELIYGTVSENQFAWQFNGGEIYMQRKQMQPLIGRTGIEFGKTFRGKDWEM
                     TALTGINYQYDLFKPTVTAFKDLAGDTYI"
BASE COUNT         1061 a          928 c          767 g         1169 t
ORIGIN      
        1 ttaatatatg tatcacctgc cagatccttg aacgctgtca cagtaggctt aaataaatcg
       61 tactgataat taatcccagt cagtgctgtc atttcccagt ctttgccgcg gaatgttttg
      121 ccaaattcaa ttcctgtgcg accaatcaat ggttgcatct gcttacgttg catatagatc
      181 tcacccccat tgaattgcca ggcaaactga ttctcagata cagtaccgta gatcaactct
      241 gtttgcggct gtatatatgt ttcgtcaggt aaggaaaaac gccaaccggc ctcagcgccc
      301 aaataccagg agtgcgagcc ataatcttgt tctggcattc ctaacgcact tgatgagtaa
      361 tgattgtcat gacgcacata tttcccgata gtatctacat agagtccaga gtcaaacata
      421 gctgaagcgt acacaccaat acccgttgat ttggttcttc ccgaccaacc agttccccgg
      481 ttattactgc ttgtaaatgt cgccgttact ccagtaaata actctccgcc ttcaattata
      541 tgtttcctgt ctgccccaat ttgcaagtgc gtataacttc cggtaaatcc atcagtagct
      601 gagcccgaac cactgttaag tctagcccat gccccagttt caccatgggt atggcgtaag
      661 tcccccatac gatcattcag attattaatt tctgtaataa agtttttata accgagatcc
      721 ataaaactct tgcctgtccg caaagcggct ttatcaggct gtatgtaaaa tccctccaga
      781 cgccattgct tattaacgtc ggtctccaac acatcaagtt gcggtgtgaa actatggaac
      841 cctgcattta ttgaaaccgg gttaagtgta aatatattgc tttgtgtatt tttcggggca
      901 gtaaccaatg acacagggtc accgccctgt tttactgaat ttgtcgtagg aataacaacc
      961 agtgtgttat tcccccctgt caatgcctgg tttaccgtca ccgtatctgc tgctttacca
     1021 tcggcattga taatgaacga actgttagtg gcctccagcg tatctgacgt cagtccccct
     1081 tgcccgaaac gattaaagct cagtgttgaa ccattcaaag tgagcgactt agccaccgac
     1141 tgcttgttta cctgccaggc agtgtccttc attgttacac ttgccaacgg cgcgttgata
     1201 tttccatagt aatttgtgga ttggttaata tcagtcgtgc caaagctaat gctggccgca
     1261 tcattggcat tgatatctcc agtgatgatc ccacccgcgc caacatttaa gctcgtgtct
     1321 tgcccgctaa gctcccagct ttgggcggaa tacatcgttc caggctggtc tgatagcaag
     1381 gaaagactcg ccccggcatt actgcttacc gtcccgtcag aaagtaatcc ggctgtagcg
     1441 gtcaattttg ccccttcacc taatgacaaa gaagattggg tgaaacggct gagctgatta
     1501 agcgtggtat ctgtagaggt tatcgtcgtc gcactatctg cactatcaat accgccggca
     1561 aaatgttcat taatagttag tgccgatcct tgttgcaact gtacatgtcc attgaaacga
     1621 ctctgatcat cttctgcggt tgcctttgac ttacccaatg ttggtttggt tgccacgcca
     1681 ttaccgtcat tgagatcaat gtaaagatcc tcactaccta atgtcacggt agaatgctcg
     1741 gcattaattc tggtattaag gctggcgttt cttgcaaggt taaattcagc attttgtaag
     1801 ttaagctccg ccatactgaa ctgtcgattt tcccaatcgt tctgagtaaa tgacactggt
     1861 tgggtcagta ctgaattgtc acccagcgcc tttagcttat ttgctacgtc ctgactgctg
     1921 acggcgtgaa caaccggatg tccttgcata aatagccgac cattttgctg ggttatactc
     1981 ccctgagtat tcactccacc atcaatagcg aaatcattct gtgttcctgc ggtaacctca
     2041 ttattgatat ttagattgcc ggtgaaatgg ccgtgccaga tattagcaac cgtggctgtt
     2101 gtatctgggc tgttgaaatc cagattaaca ttagccatgg agccactgtt agtcagtata
     2161 gctccatcat cagccccgtt aagtttatga aaagtcaaac tgtttccatt aatgtcgagc
     2221 ttgccgccat gatagcccca tttgatatta tccggattaa tttgtttgtc gtcgtttagc
     2281 accagagtag gacgcccact tacaatactg acagaactga acgcctggct cagtccgtta
     2341 tcatcagcac gctgtgccaa tataacggtt ccgtctccca cacttaagct tcctgggttt
     2401 acaccggtac cgttaatttt taacgtaccc gtacccagtt tatgcaaact atcaccagta
     2461 accccattaa tctgccagtc aacaagatgt tcaccattaa caataattcc gccgcctttc
     2521 catgtctgag tatcaacggg cttgacaata taatcatcat caaaagtaag tgtgccagcc
     2581 ccctgattaa cggtattttc taacactaca gtaccgccgc cctgaaaagt tagatcttta
     2641 ccactattaa gcgaagcagg ccatgttgcc cccaaatttc catgcatttc ccatgatgtg
     2701 cttccctggg ttaatgaacc aagtcccgtt ttttcgtcat atgaccaatg aatatcccct
     2761 tcaccttgtt ttgtatttac tggtgcatcg gcatcttgtt tcatcgtatt ttttacatca
     2821 ccagcaggaa ttattgtgta ccagttagta ttgccattga cccctgcata tgaattgaga
     2881 acggctaaca aaacccattt attttggtct gcatcccagg caaaaagagg agagccgctg
     2941 tcaccgggag taccgtaggt tgagagtgga gtattagtgg tattcgtgac aaaactccag
     3001 tcagatatag ccggcgtatt tactattcca cctgttttat acgagtaggc tcccattaaa
     3061 taggtgattt ttccgttagt atcttttaca tattgagttc ccgtacctac tcgatagaac
     3121 acagggaaac ggtcagcatt ttgataggtt cccttagctg tacctgcatt ggttatatcc
     3181 agcggtgtaa cctcggtgac aattttgttg agacggggag tgtggaaatc tctccagtgg
     3241 ttatttctgt caacaagagc ataggaggaa tcatccgcat agccaaattt cacatattga
     3301 taaccaccat tgtgcttaac gctgacgaca tattgtggtg aaaccagtgt tgcaacccca
     3361 gtcgagtcat tagagctgaa gtcaatcatt ggcgctttat ttagcgtcat gacgggattg
     3421 ccatttttat catatagagg tacatcttcc gcgccggggc ggaactctcc cttattctca
     3481 gcaaaatcac gatatgtttg ataggaagta gtggagctaa cgacggaagc acacgatgga
     3541 aagctgtatc ctcccaccat tatgcttatc aatagcaatg ttgataattt tgacttcaaa
     3601 agaaatgtcg aaatactgtt tctttttata ttttttacca cttccgacac agcaacaagt
     3661 tcacctctat gatttttctt taacatatat attttattca catcacaccc cccaacaggt
     3721 gaaaaatatt taattatata ttcaccttac attgggatag tcaagaggtg acgggacgtg
     3781 agcaaattaa ttatcaaaca aacatattta ttgatggcat cacaaaataa ataacaaaat
     3841 cggataaata aaacaaaata aaaatacaag aataagataa caaattatca aaaaaaacaa
     3901 aaaaaatgat tttttttggt attta
//
LOCUS       DAHHTG010000236         3912 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.240, whole genome shotgun sequence.
ACCESSION   DAHHTG010000236 DAHHTG010000000
VERSION     DAHHTG010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3912)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3912)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3912
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.240"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            356..562
                     /locus_tag="NBI74_004489"
     CDS             356..562
                     /locus_tag="NBI74_004489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001564245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family transcriptional regulator"
                     /protein_id="HCD1934707.1"
                     /translation="MTTPVSLMDDQMVDMAFITQLTGLTDKWFYKLIRDGAFPAPIKL
                     GRSSRWLKSEVEAWLQARIAQSRP"
     gene            657..1259
                     /locus_tag="NBI74_004490"
     CDS             657..1259
                     /locus_tag="NBI74_004490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001546339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1934708.1"
                     /translation="MKQQYQTRYELLHESYQKWLTGFTRHAVSWGVCHPNIYYFHNLT
                     PGWVSFNGEKPEIAIVPQSLHRLIYGPDKRATPPLDDDLIVNLCTSEHLLVHHPMLEG
                     ILLSECERLRQRSLANKLISLFRQFGGTELRLKLVWLCWLDLMTGNSLEDWKENLKRK
                     SEKELEEWIINRQRQSAALTDLMDQYVLLAYRTTVDDNRN"
     gene            2460..3377
                     /locus_tag="NBI74_004491"
     CDS             2460..3377
                     /locus_tag="NBI74_004491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016230784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1934709.1"
                     /translation="MVQKILSDKVMNERTNAYYSYYLGERNISVLPLNVYDPPERFIA
                     YIKKNRENLNITLSDFELEQIISGMRLKAQAFLVPLEKISWIAGSERACLFSWYLLMQ
                     FIQNNRAKISADLLQKNKLYLKEEYLEGNAFPSDSSTQFRQILRVLDILSDKNLRDEW
                     IIQTKDRWMRAFKSKSPFSYLLPENEHECIWTWNYLKGKNIALEKLASFPGSADIYHA
                     IHLSFDIWVTCPLTSPDDIKNFRNSFNKAKAQRKYKKMQEDKVNVQFFLDAETRAQLK
                     ELSRVRRLSTGEILHDLIVEEYKRYRHSR"
     gene            3462..>3912
                     /locus_tag="NBI74_004492"
     CDS             3462..>3912
                     /locus_tag="NBI74_004492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosome-binding GTPase"
                     /protein_id="HCD1934710.1"
                     /translation="MNPSDAIEAIEKPLSSLPYSLSRHIQEHLRKLTSHEPVIGIMGK
                     SGAGKSSLCNALFQGEVTPVSDVHAGTREVRRFRLSGHGHSMIITDLPGVGESRDRDA
                     EYEALYRDILPELDLVLWLIKADDRALSVDEYFWRHILHRGHQQVLFV"
BASE COUNT         1018 a          979 c          896 g         1019 t
ORIGIN      
        1 ttataatacc aatcagacag tcttatccca ccagtgataa ctgtcgcctg cccacaggtc
       61 acaggctgat ataccacaac cgccttcaaa aacaactctg caattatcag aacgccagtt
      121 ttccccttgt ctcacgaaag tcctccctcc tttatcgcgt gcggatgcat cgtttcgcaa
      181 caaccacccg acataccggt acggctcagc caccgcaatc cccccgttta tctcctgacg
      241 gtcacattcc cgattatttt ttacgtttct ccccggttgt tatgccggtg aaggtggtgc
      301 gttgttttca tcaccaacgc cggttgagat taacaacatc cggaggaaca ttctcatgac
      361 caccccagtt tcactgatgg atgaccagat ggtcgacatg gcgtttatca ctcaactgac
      421 tggcctgacc gataagtggt tttacaaact catcagggat ggggcctttc cggcccccat
      481 caagctgggc cgcagttccc gatggctgaa aagtgaagtg gaagcctggc tgcaggcgcg
      541 tattgcacag tcccgtccgt aatttctgac cgttatccgt tcacccgcag cagacgtaat
      601 ccccggcctg ccggcggcat tctgctgcct gttatatccc cgtgaggaat atgaaaatga
      661 aacaacagta tcagacccgt tacgaactgc tccacgaaag ctaccagaaa tggctgaccg
      721 gcttcacccg gcacgccgta tcctggggcg tgtgtcatcc gaatatctac tacttccata
      781 atctgacgcc cgggtgggtg tcattcaatg gtgaaaaacc ggagattgcc atcgtacccc
      841 agagcctgca ccggctgatt tatggccctg acaaacgggc cacgccgccc ctggatgatg
      901 atctgatagt gaatttatgc accagtgaac atctgctggt tcatcatccg atgctggaag
      961 gcattctcct gtctgaatgt gaacgtctga ggcagcgttc tctggcaaat aaactgatca
     1021 gcctcttccg tcagtttggt ggcacggagt tgcgcctcaa actggtctgg ctttgctggc
     1081 ttgatttaat gaccggaaac agccttgaag actggaaaga gaacctgaaa cggaaatcag
     1141 aaaaagagct ggaggaatgg atcatcaacc gtcagaggca gagcgcagca ctgacggatc
     1201 tgatggatca gtacgtgctc ctggcatacc gcacaacggt tgacgataac cgcaactgat
     1261 gccggttgct tccggaaggt gtgacaaaat acggaaatga tgatgctccc ggccccgcat
     1321 gctcacctgc agagcgtgtt cgtcgtcatc atactgagcc atgacgggcg tcttaagggc
     1381 atcctgtcat tgctgagcag gcaacaccac cagaggagcc acggacaggg atgttcttta
     1441 cagcccgatg cacgccaggt tctcagcaga ctgaatgtgc aggtgcggtt attcaccaga
     1501 aatggcatgc tgcggtacat acccatcctg gtgacaatat gtacgtggcg acagcgatcc
     1561 cggcactgtg gaagagactc actcagcctg caggatattc ttttcggcat ccgggaatgt
     1621 ccgcctgaat gcaaacagga tgatatttga atccgtaata ctgttctttc atcataaata
     1681 atttatgcag atacagttaa ccgtcacagt agcaggtttg ttttcccggg aaataatatc
     1741 cgtcagactg atgccggaac atatctgaaa cacgttatga tgttttgtta tcagaaatgt
     1801 tgtatattct tatccgtgac ggcagtttaa tgcactctgg tatcacctga taatgcatat
     1861 cctgcgatga ttcgttatat ttacgggaca cgtctctttt cattgtgctc cttttcagac
     1921 ttcattttcc ataagtgttc cccggcataa aggaggtacc tttctggcgt ttataactct
     1981 ctttatcggt gacgccggta atacagggaa taccacaccg acaaacgagg tattaacgga
     2041 cgatatttca gtgtccataa ttttgctgtc cggttaaata acgtcagtct gctgccctct
     2101 gactgtaatc atcaaagccg gaatgcatac caggttacaa cgtaaaaaaa gtgatgctgg
     2161 cctccggtgc agctggcggg cgaaacatgt ttacctttca ataccccctg tctgcagtct
     2221 ccactcttcc atcctcttaa gctacgtcgg ccacatgaat gcacttccat gataacaggt
     2281 attcctgtca gcataacgaa gccggttacc ttcccttcct cctgcatttc ctgtcataat
     2341 gactatacag tgcgcagatt gcctttttat tgaccacctt aatataccat tataaacttc
     2401 tttttatcat gttattatca ccttgttatg tttaattttt aagcaaccgg agaaagggga
     2461 tggttcagaa aattttgtcg gataaggtta tgaatgaaag aacaaatgcg tattactctt
     2521 attatcttgg agagcgtaat atctccgtac tgccccttaa tgtttatgat cccccggaac
     2581 gttttatcgc ttacataaaa aaaaacagag aaaatctgaa catcacactt tcagattttg
     2641 aacttgaaca aatcatttct ggtatgcgtc tgaaggccca ggccttcctg gtccccctgg
     2701 aaaaaatatc ctggatcgcg ggcagtgaac gcgcatgctt attttcctgg tatctgttga
     2761 tgcagtttat ccagaataac agggcaaaaa taagcgcaga cttgcttcaa aagaataaac
     2821 tgtatcttaa agaagagtat ctggaaggga atgccttccc gtcggactca tcgacacagt
     2881 tcagacaaat actcagagtt ctcgatattc tttctgataa gaatttgcgg gatgagtgga
     2941 taatccagac caaagacaga tggatgcggg catttaaatc aaagagtcct ttcagttacc
     3001 tcctcccgga gaacgaacat gaatgcatat ggacatggaa ttatttgaag ggaaaaaata
     3061 ttgcactgga aaaactggcc agttttcctg gttctgctga tatttaccac gcaattcacc
     3121 tttcatttga catatgggtt acctgtccac tcacctcacc tgacgatata aaaaatttca
     3181 ggaacagttt caataaggcc aaggcgcagc gcaagtacaa aaaaatgcag gaagataaag
     3241 tcaatgtaca gttttttctt gacgcagaaa ccagggcgca gctcaaagaa ttatcgaggg
     3301 tcagaagact cagtaccggc gaaatactgc atgacctgat tgttgaagag tataaaaggt
     3361 accgacattc cagataattt cagacatata ttatctctgt gagtcccccg ccacctttcc
     3421 ggtgtgcggg agttttgtct tttttcaccg ggaatacatg tatgaatccg tctgatgcca
     3481 ttgaggcaat tgaaaaaccg ctctcctccc tgccttactc actttcccgt cacatccagg
     3541 aacatctgcg caaactcacc agtcacgaac ccgtgattgg cattatgggt aaaagcgggg
     3601 ccggtaaatc ctcactctgt aatgcactgt ttcaggggga ggtcaccccg gtcagtgatg
     3661 ttcacgccgg cacccgggaa gtgcggcgct tccgtctgag tggccacggt cacagcatga
     3721 ttatcactga cctgcccggg gtgggcgaga gccgggacag ggatgcagag tatgaagccc
     3781 tgtaccgtga cattctgcct gaacttgacc tggtactgtg gctgattaaa gccgatgacc
     3841 gtgccctgtc tgtggatgag tatttctggc gacacatcct gcaccgggga catcagcagg
     3901 tgctgtttgt gg
//
LOCUS       DAHHTG010000237         3880 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.241, whole genome shotgun sequence.
ACCESSION   DAHHTG010000237 DAHHTG010000000
VERSION     DAHHTG010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3880)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3880)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3880
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.241"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            17..685
                     /locus_tag="NBI74_004493"
     CDS             17..685
                     /locus_tag="NBI74_004493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1934711.1"
                     /translation="MNEQIKQDIDLIEILFYLKKKIRVILFTIAICMAMVLLFLYINK
                     DNIKVSYSLKINQTTPGILVSCDSNNNFACQTTMTEDVIQRITTFFQTSPDVKNREIK
                     LEWSGDKRDLPTAEAEISRVQASIIKWYTSEYHNGRQVLDEIQTPSAINSELYTKMIY
                     LTRNWSLYPNGDGCVTISSPEIKNKYPAAICLALGFFLSIVISVMFCLVKKMVDEYQQ
                     NSGQ"
     gene            complement(928..1623)
                     /locus_tag="NBI74_004494"
     CDS             complement(928..1623)
                     /locus_tag="NBI74_004494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414842.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein C"
                     /protein_id="HCD1934712.1"
                     /translation="MDNRSEFLNNVAQALGRPLRLEPQAEDAPLNNYANERLTQLTQQ
                     QRCDAFIQFASDVMLTRCELTSEAKAAEAAIRLCKELGDQSVVISGDTRLEELGISER
                     LQQECNAVVWDPAKGIENISQAEQAIVGVVYAEYGLTESGGVVLFSAAGRGRSLSLLP
                     ESSLFILRKSTILPRVAQLAEKLHQKAQAGERMPSCINIISGPSSTADIELIKVVGVH
                     GPVKAVYLIIEDC"
     gene            complement(1616..3043)
                     /locus_tag="NBI74_004495"
     CDS             complement(1616..3043)
                     /locus_tag="NBI74_004495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster-binding protein"
                     /protein_id="HCD1934713.1"
                     /translation="MSIKTSNTDFKTRIRQQIEDPIMRKAVANAQQRIGANRQKMVDE
                     LGHWEEWRDRAAQIRDHVLSNLDAYLYQLSEKVTQNGGHVYFAKTKEDATRYILQVAQ
                     RKNARKVVKSKSMVTEEIGVNHVLQDAGIQVIETDLGEYILQLDQDPPSHVVVPAIHK
                     DRHQIRRVLHERLGYEGPETPEAMTLFIRQKIREDFLSAEIGITGCNFAVAETGSVCL
                     VTNEGNARMCTTLPKTHIAVMGMERIAPTFAEVDVLITMLARSAVGARLTGYNTWLTG
                     PREAGHVDGPEEFHLVIVDNGRSEVLASEFRDVLRCIRCGACMNTCPAYRHIGGHGYG
                     SIYPGPIGAVISPLLGGYKDFKDLPYACSLCTACDSVCPVRIPLSKLILRHRRVMAEK
                     GITAKAEQRAIKMFAYANSHPGLWKVGMMAGAHAASWFINGGKTPLKFGAISDWMEAR
                     DLPEADGESFRSWFKKHQAQENKNG"
     gene            complement(3054..3773)
                     /locus_tag="NBI74_004496"
     CDS             complement(3054..3773)
                     /locus_tag="NBI74_004496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HCD1934714.1"
                     /translation="MNVNFFVTCIGDALKSRMARDSVLLLEKLGCRVNFPEKQGCCGQ
                     PAINSGYIKEAIPGMKNLIAALEDNDDPIISPAGSCTYAVKSYPTYLADEPEWASRAE
                     KVAARMQDLTSFIVNTLGVVDVGASLQGRAVYHPSCSLARKLGVKDEPLTLLKNVRGL
                     ELLTFAEQDTCCGFGGTFSVKMAEISGEMVKEKVAHLMEVRPEYLIGADVSCLLNISG
                     RLQREGQKVKVMHIAEVLMSR"
BASE COUNT         1018 a         1012 c          823 g         1027 t
ORIGIN      
        1 attttctgga tatattatga atgaacaaat caaacaggat atcgatctga ttgagatttt
       61 attttatctg aagaaaaaga ttcgtgttat cctttttact atagctatct gtatggcaat
      121 ggtgctgttg tttctgtata tcaataaaga taatataaaa gtgagttaca gcctaaaaat
      181 aaatcagaca acaccaggca tacttgttag ctgtgatagc aataataact ttgcctgtca
      241 gaccacaatg actgaagatg ttattcagcg aattactaca ttttttcaga ccagcccaga
      301 tgtcaagaac agagaaataa agctggaatg gtcaggagat aagagagatt taccaactgc
      361 tgaagcggaa atatctcgcg tgcaggcgtc tatcattaaa tggtatacgt cagaatacca
      421 taatggcagg caagttctcg atgagataca aacgccttca gcaattaaca gtgagcttta
      481 tacaaaaatg atatatctga ccaggaactg gtcactgtat ccgaacggtg atggctgtgt
      541 cactataagc tcaccagaaa taaaaaataa ataccctgct gccatttgcc tggctctggg
      601 attttttcta agtattgtga tttctgtaat gttttgcctt gtcaaaaaaa tggtagatga
      661 ataccaacaa aactctgggc agtaataaac agcattctga cagagagtgc ctgtataaaa
      721 ctatagtgac agtgaccggg aagggagccc gtcttcagga atggattcct gtctttttaa
      781 ctgtaaatat cctggcatta ttaatgtcag gatatttaca gaagagcagc ataaccatga
      841 cggaacgggg ttaagtcagg ttgtccttaa cgctattcac aacctcgtat accgccttca
      901 ggtttatgtc cagactacat atctctctca gcaatcctca ataatcagat acaccgcttt
      961 caccggacca tgaaccccga cgactttgat aagctcaatg tccgccgttg aactgggacc
     1021 actgatgatg ttaatacatg acggcattcg ttcaccggcc tgcgctttct gatgcaattt
     1081 ttctgcaagt tgcgctacac gcggcaggat tgtgctttta cgcaggataa aaagagaaga
     1141 ttccgggagc aggctcaatg aacgcccgcg cccggcggcg gaaaaaagaa ccacgcctcc
     1201 cgattcggtt aaaccatatt cagcatatac gacgcccact atagcctgct ctgcttgtga
     1261 tatgttctca atacctttcg ccggatccca aacaacggca ttgcattcct gctgcaaacg
     1321 ttcgctaatc cccaattcct ccagcctcgt gtcaccgcta atcacgaccg actgatctcc
     1381 cagctcttta cacagacgta ttgcagcttc tgccgccttc gcctcgctgg tcagttcaca
     1441 gcgcgtcaac ataacatcgc tggcaaactg gataaacgcg tcacagcgct gttgttgggt
     1501 aagttgggta agccgctcgt tagcatagtt gttaagcggc gcatcttctg cttgcggttc
     1561 aagtcgcagc gggcgaccca gtgcctgagc aacgttattc aaaaattcgc tccgattatc
     1621 cattcttatt ctcctgcgcc tgatgtttct taaaccaact acggaaactc tctccgtcag
     1681 cttcaggaag atcgcgtgct tccatccagt cgctaatcgc gccaaatttg agtggtgttt
     1741 tgccgccatt gataaaccag cttgccgcat gagcaccagc catcatccca actttccaca
     1801 atcctggatg actattggca taagcgaaca tttttatcgc ccgttgctct gcttttgcgg
     1861 tgatcccttt ttcagccatc acccgacgat gacgcaaaat cagttttgac agcggaatac
     1921 gcaccggaca cacgctgtca caagcggtgc ataaagagca ggcgtagggt aaatctttaa
     1981 aatctttata gccgccaagt aacggggaaa tcaccgcacc aattggccct ggataaatag
     2041 agccatatcc atgaccgcca atatggcgat atgccggaca agtattcata caagccccgc
     2101 aacgaataca gcgcagcaca tcccgaaatt cagaggccag cacctcagaa cgcccgttat
     2161 cgacaataac cagatgaaac tcttcaggac catcaacgtg ccccgcttcg cgtggtcctg
     2221 tcagccaggt gttgtatccc gtcaaacgtg caccaacggc actgcgcgcc agcatggtga
     2281 tcaatacatc tacctctgca aacgtgggag caatacgctc cattcccatc actgcaatat
     2341 gcgttttagg cagcgtggta cacattcgcg cattaccttc attggtcacc aggcataccg
     2401 aaccggtctc tgccaccgcg aaattacagc cggtaatacc tatttcagca ctgaggaaat
     2461 cttcgcggat tttttgccga atgaataagg tcatcgcttc aggcgtttcc ggcccctcat
     2521 agcccagacg ttcgtgtagc actcgacgga tctgatggcg atctttatga attgccggga
     2581 ccacaacatg agagggcgga tcttgatcca gctggagaat atattcaccc agatcggttt
     2641 caatcacctg aatcccggca tcctgcaaca catgattgac accaatctct tcggtcacca
     2701 tcgatttaga tttcactacc ttccgggcat ttttgcgttg ggcaacctgt aaaatgtagc
     2761 gggtggcgtc ttctttggtt tttgcaaaat agacgtgacc gccgttttgt gtcacttttt
     2821 ctgagagctg gtacagataa gcgtcgagat tactcagaac atgatcacgt atctgggcgg
     2881 cccgatcgcg ccactcctcc cagtgcccca attcatcgac cattttttgc cgatttgccc
     2941 caatacgctg ctgcgcgttt gccaccgctt tgcgcatgat cggatcttca atttgctgac
     3001 ggatgcgtgt cttaaaatct gtattactgg ttttgatcga catctttata tcctcagcgg
     3061 ctcatcagca cttcagcaat atgcatcact ttgactttct gcccttcccg ttgtaatcgt
     3121 ccactgatgt tcagcaggca actcacgtca gcaccaatta aatactcagg gcggacttcc
     3181 atcaggtgcg caaccttttc tttcaccatc tcgccggata tttcggccat tttgaccgag
     3241 aacgtgccgc caaatccgca gcaggtatcc tgttcagcaa aggtcaacag ctccagtcca
     3301 cgcacatttt tcagcagcgt aagtggttcg tccttcaccc ccagcttacg ggccaggcta
     3361 caagatgggt gatacaccgc tctcccttgc aaactggcac ctacatcgac tacccctaat
     3421 gtattaacaa taaaagaggt gagatcctgc atacgcgcgg caaccttttc ggcacgtgat
     3481 gcccattcag gttcatccgc cagatacgtc gggtaacttt ttacggcata ggtgcaagag
     3541 ccagccggtg aaataatggg atcgtcgtta tcctccagtg cggcgatcag atttttcatc
     3601 cctggaatcg cttctttgat ataaccacta ttgatcgcag gctgaccgca gcatccctgt
     3661 ttctccggga aatttacgcg acagccgagt ttttccagta gcagcacgga gtctcgtgcc
     3721 attcttgatt tcagggcgtc accaatacag gtgacaaaga aattgacatt cacaactaat
     3781 actccattac ttcatgccca tttatgcccg atggttccct ccttccgggg gatcaatcat
     3841 caataaatat ggcgttcttt tttaattggc tagcaacata
//
LOCUS       DAHHTG010000238         3846 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.242, whole genome shotgun sequence.
ACCESSION   DAHHTG010000238 DAHHTG010000000
VERSION     DAHHTG010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3846)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3846)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3846
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.242"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            165..1403
                     /gene="alaC"
                     /locus_tag="NBI74_004497"
     CDS             165..1403
                     /gene="alaC"
                     /locus_tag="NBI74_004497"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase"
                     /protein_id="HCD1934715.1"
                     /translation="MADTRPERRFTRIDRLPPYVFNITAELKMAARRRGEDIIDFSMG
                     NPDGATPPHIVEKLCTVAQRPDTHGYSTSRGIPRLRRAISRWYQDRYDVEIDPESEAI
                     VTIGSKEGLAHLMLATLDHGDTVLVPNPSYPIHIYGAVIAGAQVRSVPLVEGVDFFNE
                     LERAIRESYPKPKMMILGFPSNPTAQCVELEFFEKVVALAKRYDVLVVHDLAYADIVY
                     DGWKAPSIMQVPGARDVAVEFFTLSKSYNMAGWRIGFMVGNKTLVSALARIKSYHDYG
                     TFTPLQVAAIAALEGDQQCVRDIAEQYKRRRDVLVKGLHEAGWMVEMPKASMYVWAKI
                     PEPYAAMGSLEFAKKLLNEAKVCVSPGIGFGDYGDTHVRFALIENRDRIRQAIRGIKA
                     MFRADGLLPASSKHIHENAE"
     gene            complement(1468..1539)
                     /gene="ypdK"
                     /locus_tag="NBI74_004498"
     CDS             complement(1468..1539)
                     /gene="ypdK"
                     /locus_tag="NBI74_004498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_077777854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YpdK"
                     /protein_id="HCD1934716.1"
                     /translation="MKYFFMGISFMVIVWAGTFALMI"
     gene            complement(1896..2816)
                     /gene="lpxP"
                     /locus_tag="NBI74_004499"
     CDS             complement(1896..2816)
                     /gene="lpxP"
                     /locus_tag="NBI74_004499"
                     /EC_number="2.3.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311285.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) palmitoleoyltransferase"
                     /protein_id="HCD1934717.1"
                     /translation="MFPKCKFSREFLHPRYWLTWFGLGVLWLWVQLPYPVLCFLGTRI
                     GTMARPFLKRRESIARKNLELCFPQHSAEEREKMIAENFRSLGMALVETGMAWFWPDS
                     RVRKWFDVEGLDNLKRAQMQNRGVMVVGVHFMSLELGGRVMGLCQPMMATYRPHNNQL
                     MEWVQTRGRMRSNKAMIGRNNLRGIVSALKKGEAVWFAPDQDYGRKGSSFAPFFAVEN
                     VATTNGTYVLSRLSGAAMLTVTMVRKADYSGYRLYITPEMEGYPTDENQAAAYMNKII
                     EKEIMRAPEQYLWIHRRFKTRPVGESSLYI"
     gene            3169..3411
                     /locus_tag="NBI74_004500"
     CDS             3169..3411
                     /locus_tag="NBI74_004500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdY family protein"
                     /protein_id="HCD1934718.1"
                     /translation="MIYLWMFLALCIVCVSGYIGQVLNMVSAISSFFGMVILAALIYY
                     FTMWLTGGNELVTGIFMFLAPACGLMIRFMVGYGRR"
     gene            complement(3488..3763)
                     /locus_tag="NBI74_004501"
     CDS             complement(3488..3763)
                     /locus_tag="NBI74_004501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCD1934719.1"
                     /translation="MKVNLMLFSLFLFVSIMACNVFAFSISGGGSEASYKETEKTSVM
                     TTTHSTKLQPSQAILFKMREDAPPLNLTEDITPTYPTKANYLIHPLR"
BASE COUNT          983 a          897 c          964 g         1002 t
ORIGIN      
        1 gataaatttc ctgtttttga agtggagttc ctcatttaaa gccattgata aaaatgttct
       61 aaaacgctgg acagacagaa attaatcagg ctattttcaa aacagccacc atcgtggcag
      121 cgtcgctcgg acggtccggg cgctaacgtt aatctgagga aattatggct gacactcgcc
      181 ctgaacgtcg ctttacgcgc attgatcgtc tcccgcccta cgtttttaac attaccgctg
      241 aactgaaaat ggctgcgcgt cggcgcggcg aagatattat cgatttcagt atgggtaacc
      301 cggacggtgc gactccgccg catatcgtcg aaaaattatg tacggtggcc cagcgcccgg
      361 acacgcatgg ttactccact tcacgcggca ttccgcggtt acgtcgcgcc atttcccgct
      421 ggtatcagga tcgctacgac gttgaaatcg acccggaatc agaagccatc gtcactattg
      481 gttcgaaaga ggggctggcg cacctgatgc tggcgacgct ggatcatggt gacacagtgc
      541 tggtgccgaa tccaagctac cccattcata tttacggcgc ggtgattgcc ggggcgcagg
      601 tacgctcagt gccgctggtg gaaggtgtcg acttcttcaa tgaactggag cgcgccattc
      661 gtgaaagtta tccgaaaccg aagatgatga tcctcggctt cccgtcaaac ccaaccgcgc
      721 aatgcgtgga actggaattc tttgagaagg ttgtggcgct ggctaaacgc tacgatgtgc
      781 tggtggtcca cgacctggcc tatgccgata tcgtctacga tggctggaaa gcgccgtcaa
      841 tcatgcaggt gccgggcgca cgcgacgtgg cggtcgagtt ctttacgctg tcgaaaagct
      901 acaacatggc gggctggcgt atcggcttta tggtcggtaa caaaacgctg gtcagcgcgt
      961 tggcacgtat taaaagctat cacgattacg gcacctttac gccgttgcag gtggcggcta
     1021 ttgcggcgct ggagggcgat caacagtgcg tgcgcgatat tgccgaacag tacaaacgcc
     1081 gccgcgatgt gctggtgaaa gggctgcatg aagcgggctg gatggtcgaa atgcctaagg
     1141 cttcgatgta tgtctgggcg aaaatcccgg aaccgtatgc tgccatgggc tcgctggaat
     1201 ttgccaagaa attgcttaac gaagcgaagg tctgtgtctc tccagggatt ggctttggcg
     1261 actacggcga cacgcatgtg cgctttgcac tgattgaaaa ccgcgaccgt attcgtcagg
     1321 cgattcgtgg gattaaagcg atgttccgtg ccgacggttt actacccgcc agcagcaaac
     1381 atattcacga aaacgcggaa taaacgcctt cgccagaaat gcaaacagga gcctaatggc
     1441 tcctgttttt cattgcacag tttgctttta gatcatcagg gcaaaagtac cggcccaaac
     1501 gatgaccata aaagaaatgc ccataaagaa atatttcact ttacatctct ccgcgtaatt
     1561 actgttacgc ctgaatgact tcattacacc tgatacagtg agttgacacc gggcaaaacg
     1621 accttaaacg tcctgtattt taccgtgggc atcatcccag aattctgagc taaactgctc
     1681 gagacggcgc taatgtacgc ctaaaactga aacgagacaa gaggcatttt cttatttact
     1741 tttagtaact tacaggtgtc tcatatcggc gacagtttaa tttctgacgg taataattag
     1801 atattcagcg aaggaaaaaa ccttaagaaa aaaagccata aaaaccatga ggttattatg
     1861 gctgatttga ggagggaaag agtgagagcc gtttgttaaa tgtataacga cgattcaccc
     1921 accgggcgcg ttttaaagcg acggtgtatc cagaggtact gctccggtgc gcgcatgatc
     1981 tctttctcga taatcttgtt catataggca gcggcttgat tttcatctgt cgggtagcct
     2041 tccatctctg gggtgatgta caaacgatat ccgctgtaat ccgcttttct taccatcgtt
     2101 acggtcaaca tggctgcgcc agagagacga gagaggacat aggtgccatt ggttgtggcg
     2161 acattttcca ccgcaaagaa cggcgcgaag gagctgcctt tacgaccata atcctgatcg
     2221 ggagcaaacc ataccgcttc acctttcttc agtgcactga caatgccgcg cagattattt
     2281 ctgccgatca tcgctttgtt agagcgcatg cgaccacggg tctggaccca ttccatcagc
     2341 tgattattat gcgggcgata ggtggccatc attggttggc aaagtcccat cacccgaccg
     2401 cccagttcca gcgacataaa atggacgccg acaaccatta cgccgcgatt ttgcatttgt
     2461 gcgcgtttaa ggttatccag cccttccaca tcaaaccatt tacgtacgcg actgtcgggc
     2521 cagaaccacg ccatgccggt ttctaccagc gccatgccga gtgaacgaaa gttttcggca
     2581 atcatcttct cgcgctcttc cgcagaatgc tgcgggaaac aaagttccag gtttttacgg
     2641 gcgatagatt cacgacgttt caggaatggt cgcgccattg tgccgatacg cgtgccgaga
     2701 aagcagagaa caggataagg aagctgtacc caaagccaga gtacaccaag cccaaaccac
     2761 gtgagccagt agcgaggatg cagaaactcg cgggaaaatt tgcattttgg aaacatagtt
     2821 accttctgta gatacgaatt gttgtatcgc agaagattcc atgtcgacag cggtacactc
     2881 aattgattag gccatcatgt acgacaatgg ttcctgtaaa gagtgtaaag actagtcagg
     2941 acataaccac gaggagcatg aatgcggtat ctgtacttta tcactcgctg actgctttca
     3001 gctactgatt attaatcgtg caaagtaaat atttttccgt acgtaacagt cttgcattta
     3061 acatgctgtt ttgattgatg cttagcaaaa attgccagag gttaaggatt gtcttactat
     3121 taaagtgact gaagttacag gttttttagc gcggcaggag gttgagctat gatttattta
     3181 tggatgtttc tcgccctgtg tattgtctgc gtaagcggat acatcggtca ggtactgaac
     3241 atggtatcgg cgatctcttc tttcttcggc atggtgatcc tcgctgcact catttattac
     3301 ttcaccatgt ggttaactgg cggtaatgaa ctggtgacgg ggatatttat gtttcttgcc
     3361 ccggcttgtg gcttgatgat tcgctttatg gtggggtatg gcaggcgata gtgggtcagg
     3421 atggctcatt tgggcaggtg ttcacatcag catcagacat gattgttcac ctgccaggtg
     3481 acagacgcta tcgcaaagga tgaatgagat aattcgcctt tgtcggataa gtaggcgtga
     3541 tgtcttctgt gaggtttaat ggtggtgcat cttctctcat cttaaacaaa atcgcctgtg
     3601 atggctgaag ttttgtagaa tgcgttgtcg tcatcactga cgttttttct gtctctttat
     3661 agctcgcctc actaccacca ccagaaatag aaaatgcaaa aacattgcat gccataatag
     3721 agacaaataa aaataagcta aaaagcatta agtttacttt catagaaaat atcctgaaaa
     3781 tggttcactg aaaacctacg atgttatggc tgtatttata tttaattaat cagtatttac
     3841 atcgat
//
LOCUS       DAHHTG010000239         3816 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.243, whole genome shotgun sequence.
ACCESSION   DAHHTG010000239 DAHHTG010000000
VERSION     DAHHTG010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3816)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3816)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3816
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.243"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            57..1676
                     /locus_tag="NBI74_004502"
     CDS             57..1676
                     /locus_tag="NBI74_004502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001460945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HCD1934720.1"
                     /translation="MAAFAAHAADDVKLKATKTNVAFSDFTPTEYSTKGKPNIIVLTM
                     DDLGYGQLPFDKGSFDPKTMENREVVDTYKIGIDKAIEAAQKSTPTLLSLMDEGVRFT
                     NGYVAHGVSGPSRAAIMTGRAPARFGVYSNTDAQDGIPLTETFLPELFQNHGYYTAAV
                     GKWHLSKISNVPVPEDKQTRDYHDNFTTFSAEEWQPQNRGFDYFMGFHAAGTAYYNSP
                     SLFKNRERVPAKGYISDQLTDEAIGVVDRAKTLDQPFMLYLAYNAPHLPNDNPAPEQY
                     QKQFNTGSQTADNYYASVYSVDQGVKRILEQLKKNGQYDNTIILFTSDNGAVIDGPLP
                     LNGAQKGYKSQTYPGGTHTPMFMWWKGKLQPGNYDKLISAMDFYPTALDAADISIPKD
                     LKLDGVSLLPWLQNKKQGEPHKNLTWITSYSHWFDEENIPFWDNYHKFVRHQSDDYPH
                     NPNTEDLSQFSYTVRNNDYSLVYTVENNQLGLYKLTDLQQKDNLAAANPQVVKEMQGV
                     VREFIDNSQPPLSEVNQEKFNNIKKALSEAK"
     gene            1728..2885
                     /locus_tag="NBI74_004503"
     CDS             1728..2885
                     /locus_tag="NBI74_004503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416014.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HCD1934721.1"
                     /translation="MHVTAKPSSFQCNLKCDYCFYLEKESQFTHEKWMDDSTLKEFIK
                     QYIAASGNQVYFTWQGGEPTLAGLDFFRKVIHYQQRYAGQKRIFNALQTNGILLNNEW
                     CAFLKEHEFLVGISIDGPQELHDRYRRSNSGNGTFAKVIAAIERLKSYQIEFNTLTVI
                     NNINVHYPLEVYHFLKSIGSKHMQFIELLETGTPNIDFSGHSENTFRIIDFSVPPTAY
                     GKFMSTIFMQWVKNDVGEIFIRQFESFVSRFLGNGHTSCIFQESCKDNLVVESNGDIY
                     ECDHFVYPQYKIGNINKSELKTMNSVQLTAQKKRISAKCQQCVYKPICNGGCPKHRIT
                     KVNNETVSYFCEGYKILFSTMVPYMNAMVELAKNRVPLYHIMDVAKQMENN"
     gene            2897..3808
                     /locus_tag="NBI74_004504"
                     /pseudo
     CDS             2897..3808
                     /locus_tag="NBI74_004504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416011.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
BASE COUNT         1163 a          842 c          799 g         1012 t
ORIGIN      
        1 ctgcattaaa gaaaagtgtc gtaagtacct cgatatcttt gatactggca tctggtatgg
       61 ctgcatttgc tgctcatgcg gcagatgatg taaagctgaa agcaaccaaa acaaacgttg
      121 cgttctcaga ctttactccg actgaataca gtaccaaagg aaagccaaat attatcgtac
      181 tgaccatgga tgatcttggt tatggacaac ttccttttga taagggatct tttgacccaa
      241 aaacaatgga aaatcgtgaa gttgtcgata cctacaaaat agggatagat aaagccattg
      301 aagctgcgca aaaatcaacg ccgacgctcc tttcattaat ggatgaaggc gtacgtttta
      361 ctaacggcta tgtggcgcac ggtgtttccg gcccctcccg cgccgcaata atgaccggtc
      421 gagcgcccgc ccgctttggt gtctattcca atactgatgc tcaggatggt attccgctaa
      481 cagaaacttt cttgcctgaa ttattccaga atcatggtta ttacactgca gcagtaggta
      541 aatggcactt atcaaaaatc agtaatgtgc cggtgccgga agataaacaa acgcgtgact
      601 atcatgacaa cttcactacc ttttctgcgg aagaatggca accgcaaaac cgtggctttg
      661 attactttat gggattccat gctgcaggaa cggcatatta caactcccct tcactgttca
      721 aaaaccgtga acgcgtcccc gcaaaaggtt atatcagcga tcagttaacc gatgaagcaa
      781 ttggcgttgt tgatcgtgcc aaaacacttg accagccttt tatgctttac ctggcttata
      841 atgctccgca cctgccaaat gataatcctg ctccggagca atatcagaag caatttaata
      901 ccggtagtca aacagcagat aattactacg cttccgttta ttctgttgat cagggtgtaa
      961 aacgcattct cgaacaactg aagaaaaacg gacagtatga caatacaatt attctcttta
     1021 cctccgataa tggtgcagtt atcgatggtc ctctgccgct gaacggggcg caaaaaggct
     1081 ataagagtca gacctatcct ggcggtactc acaccccaat gtttatgtgg tggaaaggaa
     1141 aacttcaacc cggtaattat gacaagctga tttccgcaat ggatttctac ccgacagctc
     1201 ttgatgcagc cgatatcagc attccaaaag accttaagct ggatggcgtt tccttgctgc
     1261 cctggttgca aaataagaaa caaggcgagc cacataaaaa tctgacctgg ataacatctt
     1321 attctcactg gtttgatgag gaaaatattc cattctggga taattaccac aaatttgtcc
     1381 gccatcagtc agacgattac ccgcataacc ccaacaccga ggacttaagc caattctctt
     1441 atacggtgag aaataacgat tattcgcttg tctatacagt agaaaataat cagttaggtc
     1501 tatacaaact gacggatcta cagcaaaaag ataaccttgc cgccgccaat ccgcaggtcg
     1561 ttaaagagat gcaaggcgtg gtaagagagt ttatcgataa cagccagcca ccgcttagcg
     1621 aggtaaatca ggagaagttt aacaatatca agaaagcact aagcgaagcg aaataactaa
     1681 acctttatgc ggcggatttt tccgccgcct tattgagcga gatagcgatg cacgttacag
     1741 ccaagccctc cagttttcaa tgtaatctca aatgtgatta ctgtttttac cttgaaaaag
     1801 agtcgcagtt tactcatgaa aaatggatgg atgacagcac tctgaaagag ttcatcaaac
     1861 aatatatcgc agcgtctggc aatcaggtct attttacctg gcaaggcggt gaacccactc
     1921 tggctggcct ggattttttc cgtaaagtta ttcactatca acaacgctat gcaggccaaa
     1981 aacgtatttt taatgcatta caaacgaatg gcattttatt gaataatgaa tggtgtgcct
     2041 ttctcaaaga acatgagttt ctggttggta tctcgatcga tggcccccaa gaactacatg
     2101 accgttacag acgcagtaat tcaggtaacg gtacttttgc aaaagtgata gcagccatcg
     2161 agcgtctgaa atcatatcaa atagagttta atacgttaac cgttattaat aacattaatg
     2221 tccattaccc tcttgaggtt tatcattttt taaaatctat cggcagtaaa catatgcaat
     2281 ttatcgaatt gctcgaaacc gggacgccga atattgattt cagtggtcat agtgagaaca
     2341 cattccgtat cattgatttt tctgtgcctc ccacggctta tggcaagttt atgtcaacca
     2401 tttttatgca atgggttaaa aacgatgtgg gtgaaatttt catccgtcag tttgaaagct
     2461 ttgtcagccg ttttttgggg aatgggcata ccagttgtat tttccaggag tcctgcaagg
     2521 ataatctggt tgttgaaagt aatggagaca tttacgaatg cgaccatttt gtctatccac
     2581 agtacaaaat tggaaacatt aataaatcgg aactcaaaac gatgaacagt gtacaactga
     2641 cagcgcaaaa aaaacggatt tcagcgaaat gtcagcaatg tgtatataaa cctatctgca
     2701 atggtggttg ccctaagcat cgtattacta aagtaaacaa tgagactgtt tcctattttt
     2761 gcgaaggtta taaaatcctt ttttcaacca tggtacctta tatgaacgcc atggtggagt
     2821 tagctaagaa cagagtaccg ctttaccaca ttatggatgt tgcaaaacaa atggagaata
     2881 attaataacg ctcccccaca gcacgattaa cgacaatatt tgggcatcgc tacaaaatgc
     2941 tcaaattcag gcgttgaaaa cgctcgacca gcgtccagcg gagaaattcg cccagcagat
     3001 gtatgagacg cgctatgctg ataaccgcac gaaattaccg caagcaaatc agattgcaca
     3061 gtttactgcc gcagatgcac tggctgccga tcgccagttg ttttcatctc cagcggatat
     3121 cacgtttgtc attgtcggta atgtcgcaga agacaaactc gtggcgttaa ttacgcgtta
     3181 cttaggatca atcaaacact ctgattcgcc attagccgca ggtaaaccat taactcgcgc
     3241 gacggacaac gcatcggtta ctgtaaaaga acaaaatgaa ccagtggcac aggtttcaca
     3301 gtggaagcgt tataattccc ggactcctgt taatctggcg acgcgtatgg cgctcgatgc
     3361 ttttaacgtc gcactggcaa aagatctacg cattaatatt cgtgaacagg catccggggc
     3421 atacagcgtt tcttctcgcc tctcggttga tcctcaggcc aaagatatca gtcatttgct
     3481 ggcttttact tgtcaaccag aacgacatga tgaactgtta acgttagcga atgaagtgat
     3541 ggttaagcgt ctggccaaag ggatcagtga gcaagaactg aacgaatacc agcaaaacgt
     3601 tcagcgcagc ctcgatatcc aacagcgtag cgttcaacaa ttagcgaaca ccattgtaaa
     3661 tagtcttatt caatatgacg atcctgcagc atggactgag caggagcaat tgttgaaaca
     3721 aatgacggta gagaatgtta acactgctgt taaacaatat ctttctcatc cggtgaatac
     3781 ctataccgga gtattattgc caaaataatc acagcc
//
LOCUS       DAHHTG010000240         3608 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.246, whole genome shotgun sequence.
ACCESSION   DAHHTG010000240 DAHHTG010000000
VERSION     DAHHTG010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3608)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3608)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3608
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.246"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            301..1428
                     /locus_tag="NBI74_004505"
     CDS             301..1428
                     /locus_tag="NBI74_004505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000229546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1934722.1"
                     /translation="MVEKMKQNSSSTLGALSAPTTWPGANARLLGLGVGLPIAPLDRL
                     AQFSAAEFERFTLEWASEYLAGQQYVNEVQQRGGAGDKGRDIVVWLDPSDAVPRRWHL
                     YQCKHYDANLGLPKAGIEVAKVIYYTFIKDYTVPTQYHFVTHKGVTSPFQDLLDDPTK
                     LKEKMLSEWATFSKQITSKHSVDLTPELEKYIKEFDFSIFHAKQPIEILAEHSKTSFH
                     LMVFGAPLIERDPPTRPPSSVAPIETVYIEQLFSVISADIKTNVRDLVDFQSSISHVK
                     LFERSRITFYCSEGLKELARDQMANQEFFNSLLVEFDDGLYHYTSDLTGTPLLRLKNT
                     VKAAQTLQLGAHPLAIHVTNKDREGICHQLANTNLINWCNP"
     gene            1425..1940
                     /locus_tag="NBI74_004506"
     CDS             1425..1940
                     /locus_tag="NBI74_004506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001293316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1934723.1"
                     /translation="MSEMQVFEGNFEQRPFNTPLEYGFRALFILDAADNVAMDLQRIV
                     SYDYLLVHTSDVPRGPKSLHPAVPHRGAELLVKRSAIQAGLHLMLSRELIKVVFAAEG
                     ILYQATNLTGRFVRLLMSQYSKELAERASWVTKQFYEYTDEELASYISQNVGQWGSEF
                     DRLTAIDLLDL"
     gene            1942..>3608
                     /locus_tag="NBI74_004507"
     CDS             1942..>3608
                     /locus_tag="NBI74_004507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000944508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1934724.1"
                     /translation="MLRIRKILLRGNSVQDAYVDFYKGANILAGESDTGKSYLVSCLD
                     YILGAEKLKKKLKEATDYTHLYVEFENDEGGVLTLKRGLEGGKLEAHDVAIQDIHGEG
                     KIIAPVRKGTSKGPDVTSILFPFAGIKEAKLRKNARGETQRFSIRTLAPIFLVDEVSI
                     IDEYSPVTGRSGYDDTARKRMFSYILTGHDDGGVTVEEKPEIVKARLMAKLEFIQDLI
                     RPLDERFSICSPKFPLTSSADDLSDQLIAQAIDEVERAAAAISDLLEGIKMETALTLK
                     IESQLMGVSEIQSRYSLLEERYHSDLKRLDFISEGSHYFTSLQEVPCSLCGQNLLHPH
                     SENAKKLMNSNEVRRSSLAEAAKIHGYLAGLQKAMSDLDRRKEALNIDRYKSKESLDG
                     MKNQIKYTFEPLLTLSKESLDLAIEQRAERDSELNDRQRWFELLKHQKDIEILLEKSS
                     QPRLEWEGLSTRTLAELCREIETVLKDWKWGAEPDVSFNEKEYDIIVDGQPRQSHGKG
                     VRAILYSAFIIGLLKYCIKKGYPHPGLVVIDSPLTSYKKKAAATVSG"
BASE COUNT         1081 a          710 c          824 g          993 t
ORIGIN      
        1 gattcaatta ttgaaatcaa aaaacatttt atcaatctac acccatcaaa gtgatggtta
       61 tgtaaagaga taaaaatgct gatgtcaaaa gaggcttatg ctcggcacag tggagtgagc
      121 tgccaaactg tcgatgactg ggtagccggt ggggcggaag tagttatgtc ccgtagcaag
      181 gttaagattt gctcttgtgt gtggggaacc ttagtcaatt actttcctgg cgcactgtgt
      241 tagattttgt aaaattttaa aagactaaag atttaatatc acttctccat ggaggttgtg
      301 gtggtagaga agatgaaaca aaattcaagt tcgacgcttg gcgcgctttc tgctcctaca
      361 acgtggccgg gtgcgaacgc tcggctgctt gggttaggtg tagggcttcc aatagccccc
      421 ttggatcgtc tggcacagtt tagcgccgcc gaatttgaaa ggtttacact cgaatgggcc
      481 tctgaatact tagctggtca gcaatatgtc aacgaagtac aacaacgtgg cggggcagga
      541 gataaaggca gagatattgt tgtatggctt gatccatctg atgctgttcc tcgaagatgg
      601 cacttatatc aatgcaaaca ttatgatgcg aatttaggcc ttcctaaggc aggcatcgaa
      661 gttgcaaaag ttatctatta tactttcatc aaggactaca cagtacctac acaataccat
      721 tttgttacac ataaaggggt aacatcgcca ttccaagacc tcctagatga tccaactaaa
      781 ctcaaagaga aaatgctatc cgagtgggca actttttcaa agcaaattac atcgaaacat
      841 agtgttgacc tgacgcctga acttgaaaaa tatattaaag agtttgattt tagtattttt
      901 catgcaaaac agcctattga gatacttgcc gaacacagta aaacctcttt tcatcttatg
      961 gtctttggcg caccattaat tgagcgggat ccaccaacta ggcccccatc atctgtggct
     1021 cccattgaaa ctgtatatat tgaacaactc ttttcagtta tcagcgctga tatcaaaacc
     1081 aacgtgcgag atttagttga ttttcaatca agtatcagcc atgtaaaact ctttgaacgg
     1141 tcccgaataa ctttttattg ctcggaaggg ctaaaggaac tcgctaggga ccaaatggcg
     1201 aatcaagaat tctttaattc tttacttgta gagtttgatg atggtctgta tcactacacc
     1261 tctgacctga cagggactcc actccttcgg ttaaaaaata ccgtcaaggc cgctcagacc
     1321 ctgcaacttg gtgcgcaccc acttgccatt cacgtaacga ataaggatcg tgaaggtata
     1381 tgccaccaac tagccaatac aaacttgatt aattggtgca atccatgagc gaaatgcaag
     1441 tatttgaagg aaactttgag cagagacctt ttaacactcc gctggagtat ggatttcgtg
     1501 ctctgttcat attggatgca gcggacaatg tagcaatgga cctacaaagg atagtttcgt
     1561 atgactatct tcttgtgcat accagtgacg tacctagggg gcctaaaagc cttcatccag
     1621 cagttcctca ccggggagca gagcttcttg ttaagcgaag cgcaattcag gcagggctgc
     1681 atttaatgct atcaagagaa ttgattaagg ttgtctttgc tgccgaaggt attttgtatc
     1741 aggctaccaa tttaacagga aggtttgtgc ggcttttgat gtcacagtat tcaaaagagc
     1801 tcgcagaacg tgcctcttgg gtcacaaagc aattctatga atatacggat gaagaacttg
     1861 cttcatatat atctcagaat gtggggcaat ggggttctga attcgaccga ttgaccgcaa
     1921 ttgatctact ggatttataa catgcttcgt ataagaaaaa tactattgcg cggaaacagc
     1981 gttcaagacg cttatgttga tttctataag ggtgcgaata ttctagcagg ggaatctgat
     2041 actgggaaaa gttaccttgt gagttgtctt gactacattc ttggtgccga aaaactcaaa
     2101 aaaaagctta aagaggccac cgattacacc catctttatg ttgaattcga aaatgatgaa
     2161 gggggggtgt taacgctcaa gcgcggtctt gaggggggga aattagaggc acatgatgtc
     2221 gctattcaag acatccatgg agaaggtaaa ataattgctc ctgtgcgtaa aggaacgagt
     2281 aaggggccag atgtgacgtc catacttttc ccttttgcgg ggatcaaaga ggcgaagctt
     2341 cgcaagaatg ctcgtggcga aacgcaacgt ttctcgattc gaacgctcgc tcctatattt
     2401 ttggtagatg aagtatccat tatcgacgaa tactcgccag taacgggacg ttccggttat
     2461 gacgacactg ctcgaaaacg catgttttca tacatcctta ccgggcacga tgatggtggt
     2521 gttaccgtag aggaaaagcc tgaaattgta aaagcgaggt tgatggcgaa gcttgaattc
     2581 atccaggatt tgattcgacc acttgatgaa cgtttctcaa tttgttcgcc caagtttcca
     2641 ttaacgagct cagcagatga tttatcagat caattgattg cacaagcaat tgatgaagtt
     2701 gagagggctg cagccgctat ttctgacttg ctcgagggaa taaaaatgga gacagcgtta
     2761 acgttaaaaa tagaatctca gttaatggga gtatctgaaa ttcaatcgag atatagccta
     2821 ttagaagaac gttaccattc cgacctgaag cggttggatt ttatttccga aggttctcac
     2881 tatttcactt cacttcagga agttccttgt tccctttgtg gacaaaattt actacatcca
     2941 cacagtgaga acgctaaaaa attaatgaac agtaatgaag tccgacgttc ttctttagct
     3001 gaagctgcaa aaattcatgg ataccttgca ggcctgcaaa aagcgatgag tgatcttgac
     3061 cgaagaaaag aagctctgaa tatagacaga tataagtcaa aagaaagctt ggatggaatg
     3121 aagaaccaga tcaaatatac ttttgagcct cttcttacac tttccaaaga aagcctagat
     3181 ttagctattg agcagcgagc tgaaagagac tctgagttga atgatcgaca gagatggttt
     3241 gagttactaa aacatcaaaa ggatatagaa atactgctgg aaaagagcag tcaaccacgg
     3301 ctcgaatggg agggattatc tacccgtacg ctggccgaac tttgtagaga aattgaaact
     3361 gttttaaaag actggaaatg gggtgccgag cctgacgttt catttaatga aaaagagtat
     3421 gacatcattg ttgatggaca acctcggcag tctcacggta aaggtgttcg tgccattctt
     3481 tattcagctt tcatcattgg attactaaaa tattgtatta aaaaaggata cccgcatcct
     3541 gggctagtcg tgattgattc accgctgaca agttataaga agaaagccgc tgctacggta
     3601 tcgggctt
//
LOCUS       DAHHTG010000241         3608 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.247, whole genome shotgun sequence.
ACCESSION   DAHHTG010000241 DAHHTG010000000
VERSION     DAHHTG010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3608)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3608)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3608
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.247"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            207..1082
                     /locus_tag="NBI74_004508"
     CDS             207..1082
                     /locus_tag="NBI74_004508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032492044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A extended-spectrum beta-lactamase
                     CTX-M-27"
                     /protein_id="HCD1934725.1"
                     /translation="MVTKRVQRMMFAAAACIPLLLGSAPLYAQTSAVQQKLAALEKSS
                     GGRLGVALIDTADNTQVLYRGDERFPMCSTSKVMAAAAVLKQSETQKQLLNQPVEIKP
                     ADLVNYNPIAEKHVNGTMTLAELSAAALQYSDNTAMNKLIAQLGGPGGVTAFARAIGD
                     ETFRLDRTEPTLNTAIPGDPRDTTTPRAMAQTLRQLTLGHALGETQRAQLVTWLKGNT
                     TGAASIRAGLPTSWTVGDKTGSGGYGTTNDIAVIWPQGRAPLVLVTYFTQPQQNAESR
                     RDVLASAARIIAEGL"
     gene            1117..2085
                     /locus_tag="NBI74_004509"
     CDS             1117..2085
                     /locus_tag="NBI74_004509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5-like element IS903B family transposase"
                     /protein_id="HCD1934726.1"
                     /translation="MKDQITYLPDNADRSVAKQKFKITNWPTYNKALINRGSITFWLD
                     DEAIQAWYESATPSSRGRPQRYSDLAITTVLVIKRVFRLTLRAAQGFIDSIFSLMNVP
                     LRCPDYSCVSRRAKSVNISFKTPTRGEIAHLVIDSTGLKVFGEGEWKVKKHGQERRRI
                     WRKLHLAVDSKTHEIICADLSLNNVTDSEAFPGLIRQTHRKIRSAAADGAYDTRLCHD
                     ELRRKKISALIPPRKGAGYWPGEYADRNRAVANQRMTGSNARWKWTTDYNRRSIAETA
                     MYRVKQLFGGSLTLRDYDGQVAEAMALVRALNKMTKAGMPESVRIA"
     gene            2142..3239
                     /locus_tag="NBI74_004510"
                     /pseudo
     CDS             2142..3239
                     /locus_tag="NBI74_004510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965681.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
     gene            complement(3307..3579)
                     /locus_tag="NBI74_004511"
                     /pseudo
     CDS             complement(3307..3579)
                     /locus_tag="NBI74_004511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_073984355.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4158 domain-containing protein"
BASE COUNT          890 a          917 c         1040 g          761 t
ORIGIN      
        1 ttgcagagtt tattaagtat cattgcagca aagatgaaat caatgattta tcaaaaatga
       61 ttgaaaggtg gttgtaaata atgttacaat gtgtgagaag cagtctaaat tcttcgtgaa
      121 atagtgattt ttgaagctaa taaaaaacac acgtggaatt tagggaatac tgatgtaaca
      181 cggattgacc gtattgggag tttgagatgg tgacaaagag agtgcaacgg atgatgttcg
      241 cggcggcggc gtgcattccg ctgctgctgg gcagcgcgcc gctttatgcg cagacgagtg
      301 cggtgcagca aaagctggcg gcgctggaga aaagcagcgg agggcggctg ggcgtcgcgc
      361 tcatcgatac cgcagataat acgcaggtgc tttatcgcgg tgatgaacgc tttccaatgt
      421 gcagtaccag taaagttatg gcggccgcgg cggtgcttaa gcagagtgaa acgcaaaagc
      481 agctgcttaa tcagcctgtc gagatcaagc ctgccgatct ggttaactac aatccgattg
      541 ccgaaaaaca cgtcaacggc acaatgacgc tggcagaact gagcgcggcc gcgttgcagt
      601 acagcgacaa taccgccatg aacaaattga ttgcccagct cggtggcccg ggaggcgtga
      661 cggcttttgc ccgcgcgatc ggcgatgaga cgtttcgtct ggatcgcact gaacctacgc
      721 tgaataccgc cattcccggc gacccgagag acaccaccac gccgcgggcg atggcgcaga
      781 cgttgcgtca gcttacgctg ggtcatgcgc tgggcgaaac ccagcgggcg cagttggtga
      841 cgtggctcaa aggcaatacg accggcgcag ccagcattcg ggccggctta ccgacgtcgt
      901 ggactgtggg tgataagacc ggcagcggcg gctacggcac caccaatgat attgcggtga
      961 tctggccgca gggtcgtgcg ccgctggttc tggtgaccta ttttacccag ccgcaacaga
     1021 acgcagagag ccgccgcgat gtgctggctt cagcggcgag aatcatcgcc gaagggctgt
     1081 aactggtttt gttgaataaa tcgaactttt gctgagttga aggatcagat cacgtatctt
     1141 cccgacaacg cagaccgttc cgtggcaaag caaaagttca aaatcaccaa ctggcccacc
     1201 tacaataaag ccctcatcaa ccgtggctcc ataactttct ggctggatga tgaagctatt
     1261 caggcctggt atgagtcagc aacaccttct tcacgaggca gacctcagcg ctattctgac
     1321 cttgccatca cgactgtgct ggtcattaaa cgcgtattca ggctgaccct gcgcgctgcg
     1381 cagggcttta ttgattccat tttttctctg atgaacgttc cgctacgctg cccggattac
     1441 agctgtgtca gcaggcgggc aaagtcggtt aatatcagtt tcaaaacgcc cacccggggt
     1501 gaaatcgcac acctggtaat tgattccacc gggctgaagg tcttcggtga aggcgagtgg
     1561 aaagtcaaaa agcatggcca ggaacgccgt cgtatatggc gtaagctgca tctggcagtt
     1621 gacagtaaaa cacatgaaat catctgcgct gacctgtcgc tgaacaacgt tacggactca
     1681 gaggccttcc ccgggttaat ccggcaaacc caccggaaaa tcaggtcagc cgccgccgat
     1741 ggagcttacg atacccggct ctgtcacgat gaactgcggc gtaagaaaat cagcgcgctt
     1801 atcccgcccc gaaaaggtgc gggttactgg cccggtgaat atgcagaccg taaccgtgca
     1861 gtggctaatc agcgaatgac cgggagtaat gcgcggtgga aatggacaac agattacaac
     1921 cgtcgctcga tagcggaaac ggcgatgtac cgggtaaaac agctgttcgg gggttcactg
     1981 acgctgcgtg actacgatgg tcaggttgcg gaggctatgg ccctggtacg agcgctgaac
     2041 aaaatgacga aagcaggtat gcctgaaagc gtgcgtattg cctgaaaaca caacccgcta
     2101 cgggggagac ttacccgaaa tctgatttat tcaacaaagc cacgcgctgg acacaccacg
     2161 atgacagcgg ttttatccct tacgccatca ccaccggcct tgattacgag gcgatgaccg
     2221 aacagcgcaa aggctatgag aactttgttt atcagaacgg cgtttacgat ctcggcacca
     2281 aaggtgatat gcgacgtaac gaacgtaacc tgatgtggaa cctcgatccc tacattcaaa
     2341 cgcactggca gctcacctca gccctggggc tggacgccgg ggcgcgctat agctcggtat
     2401 ggtttgattc caacgatcgt tatgttgtag gtaaaaatgg tgatgatagc ggcgaagcga
     2461 gctatcacaa gtggcttccg gcggcagcgc tgaagtatgc catgacgccg cagtggaacg
     2521 tttacctctc tgccgggagg ggatttgaaa cgccgaccat taacgagctg tcctatcgtc
     2581 ccgatggtca gtccgggctt aactttgctc tgcaaccggc cactaacacc accgttgaag
     2641 caggcagtaa atggcaggtg ggcaccggga tggcgagcct gagcgtcttc aatacggaca
     2701 cgaaaaatga aattgtcgtt gccgccagcg acaatggccg tacgtcctat cagaacgcgg
     2761 gcaagacgcg ccgccgcggg gtcgaactgg gatgggatca gcaatttgcg cccgcctggc
     2821 gcgccaaact ggcgtggacg ctgctggatg cgacctaccg tgaaaacgcc ggcgatgcta
     2881 ttcagagcgg caatcggatc ccggggattg cacgtaatag cgcctatgcc tcctttggct
     2941 gggtgccgga agagggatgg tacgctggcg ctgaagtccg ctatatgagc gatattcagg
     3001 tcaacgatgc caatagcgag caggctccag cctatacggt gacggcactg aataccggct
     3061 ataaatatgt gctagataac tggaccgtcg acctctatac gcgcgttgat aacctgtttg
     3121 ataagcgcta cgtcggctct gttatcgtca acgagagcaa cggacgctat tatgaaccgg
     3181 cgccgggccg taactatggc gttgggctat cggtcagcta tcagtttgat tcgctgtaac
     3241 aatacggctg ccggagagtg ctattgaggt gaggctccgg cagcgggtaa aggcgttgat
     3301 tcctggtaaa agacgggtaa ggcgaaatgt ccagggccag gcaaattcac gatactgata
     3361 gtgctgacgt atcagcgctg catgctcacg gcgggtattt tccctctgac cgtattctgc
     3421 aagaacggtg atatcacgaa tcccgagctg tctggcggta aaatgccgga cgccggaagg
     3481 aatatgattc atatcggtga ggaaggtgcc cagaaaacgg acacatccaa tttgcagggc
     3541 aatgcccaga cggttgtgat cacctctgct ttttccgata aattccttgt ctgcttcatc
     3601 aaggtgaa
//
LOCUS       DAHHTG010000242         3573 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.248, whole genome shotgun sequence.
ACCESSION   DAHHTG010000242 DAHHTG010000000
VERSION     DAHHTG010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3573)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3573)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3573
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.248"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            complement(<1..267)
                     /locus_tag="NBI74_004512"
     CDS             complement(<1..267)
                     /locus_tag="NBI74_004512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1934727.1"
                     /translation="MSENDIIIRTHYESPLRMHIDSDILTPSSEPISQFARQLITLLD
                     TSDLSSMLSYCVTQEFTVSCRKISHNCYSTALFIINFATSPIHAE"
     gene            791..2719
                     /gene="thrS"
                     /locus_tag="NBI74_004513"
     CDS             791..2719
                     /gene="thrS"
                     /locus_tag="NBI74_004513"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006894721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HCD1934728.1"
                     /translation="MPVITLPDGSQRHYDHAVSPMDVALDIGPGLAKACIAGRVNGEL
                     VDACDLIENDAQLSIITAKDEEGLEIIRHSCAHLLGHAIKQLWPHTKMAIGPVIDNGF
                     YYDVDLDRTLTQEDVEALEKRMHELAEKNYDVIKKKVSWHEARETFANRGESYKVSIL
                     DENIAHDDKPGLYFHEEYVDMCRGPHVPNMRFCHHFKLMKTAGAYWRGDSNNKMLQRI
                     YGTAWADKKALNAYLQRLEEAAKRDHRKIGKQLDLYHMQEEAPGMVFWHNDGWTIFRE
                     LEVFVRSKLKEYQYQEVKGPFMMDRVLWEKTGHWDNYKDAMFTTSSENREYCIKPMNC
                     PGHVQIFNQGLKSYRDLPLRMAEFGSCHRNEPSGSLHGLMRVRGFTQDDAHIFCTEEQ
                     IRDEVNGCIRLVYDMYSTFGFEKIVVKLSTRPEKRIGSDEMWDRAEADLAVALEENNI
                     PFEYQLGEGAFYGPKIEFTLYDCLDRAWQCGTVQLDFSLPSRLSASYVGEDNERKVPV
                     MIHRAILGSMERFIGILTEEFAGFFPTWLAPVQVVIMNITDSQSDYVNELTQKLSNAG
                     IRVKADLRNEKIGFKIREHTLRRVPYMLVCGDKEVESGKVAVRTRRGKDLGSMDVNEV
                     IEKLQQEIRSRSLKQLEE"
     gene            2723..3265
                     /gene="infC"
                     /locus_tag="NBI74_004514"
     CDS             2723..3265
                     /gene="infC"
                     /locus_tag="NBI74_004514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HCD1934729.1"
                     /translation="MKGGKRVQTARPNRINGEIRAQEVRLTGLEGEQLGIVSLREALE
                     KAEEAGVDLVEISPNAEPPVCRIMDYGKFLYEKSKSSKEQKKKQKVIQVKEIKFRPGT
                     DEGDYQVKLRSLIRFLEEGDKAKITLRFRGREMAHQQIGMEVLNRVKDDLQELAVVES
                     FPTKIEGRQMIMVLAPKKKQ"
     gene            3362..3559
                     /gene="rpmI"
                     /locus_tag="NBI74_004515"
     CDS             3362..3559
                     /gene="rpmI"
                     /locus_tag="NBI74_004515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006708231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HCD1934730.1"
                     /translation="MPKIKTVRGAAKRFKKTGKGGFKHKHANLRHILTKKATKRKRHL
                     RPKAMVSKGDLGLVIACLPYA"
BASE COUNT          998 a          779 c          902 g          894 t
ORIGIN      
        1 ttctgcatgg atgggtgaag tggcaaagtt aatgataaaa agggcagtgg aataacaatt
       61 gtgtgatatt tttcgacagc ttacggtaaa ttcctgagta acacagtatg acagcatcga
      121 acttaaatca gaggtatcaa gtagggtgat gagctggcgc gcaaattgac taataggctc
      181 tgatgaaggc gttagtatgt cgctatcgat gtgcattcta agaggagact cataatgagt
      241 tctgataatg atatcgtttt cactcataat ctattcgcct tatatatgtc tggagatttt
      301 gaggatgatg attaattatt atcgccagaa cgaaatgcgc acgaaaaatt gctataagaa
      361 ttgtgtcact tttatcattg cgatatgacc acttcgtcac tgaatctcat gatcgtttaa
      421 ataccaccgc caaataacgc aacgaacttt gctggattca gtttatgctg ctgtaaatcc
      481 gctcgtagta aacctttcag acactctgtg atgatatcag tggttcttta agcgttttgc
      541 tggtgtactc actacaaacg aattgcgaat cgaatcaatg tgaaacggaa aggtacaatc
      601 tccctttttt tagtgttaac atcgttcaac cgggtggaag gtgagaggtt aatgtctttc
      661 atttgtgtac ctgattgcga accaatttag catttgttgg ctagatggtt tcgcaatgaa
      721 ctgttaataa acaaattttt ctttgtatgt gatctttcgt gtgggtcacc actgcaaata
      781 aggatatata atgcctgtta taactcttcc tgatggcagc caacgccatt acgaccacgc
      841 tgtaagcccc atggatgttg cgctggacat tggtccaggt ctggcgaaag cctgtatcgc
      901 agggcgcgtt aatggcgaac tggttgatgc ttgcgatctg attgaaaacg acgcacaact
      961 gtcgatcatt accgccaaag acgaagaagg tctggagatc attcgtcact cctgtgcgca
     1021 cctgttaggg cacgcgatta aacaactttg gccgcatacc aaaatggcaa tcggcccggt
     1081 tattgacaac ggtttttatt acgacgttga tcttgaccgc acgttaaccc aggaagatgt
     1141 cgaagcactc gagaagcgga tgcatgagct tgctgagaaa aactacgacg tcattaagaa
     1201 gaaagtcagc tggcatgaag cgcgtgaaac tttcgccaac cgtggggaga gctacaaagt
     1261 ctccattctt gacgaaaaca tcgcccatga tgacaagcca ggtctgtact tccatgaaga
     1321 atatgtcgat atgtgccgcg gtccgcacgt accgaacatg cgtttctgcc atcatttcaa
     1381 actaatgaaa acggcagggg cttactggcg tggcgacagc aacaacaaaa tgttgcaacg
     1441 tatttacggt acggcgtggg cagacaaaaa agcgcttaac gcttacctgc aacgcctgga
     1501 agaagccgcg aaacgcgacc accgtaaaat cggtaaacag ctcgacctgt accatatgca
     1561 ggaagaagcg ccgggtatgg tattctggca caacgacggc tggaccatct tccgtgaact
     1621 ggaagtgttt gttcgttcta aactgaaaga gtaccagtat caggaagtta aaggtccgtt
     1681 catgatggac cgtgtcctgt gggaaaaaac cggtcactgg gacaactaca aagatgcaat
     1741 gttcaccacg tcttctgaga accgtgaata ctgcattaag ccgatgaact gcccggggca
     1801 cgtacaaatt ttcaaccagg ggctgaagtc ttatcgcgat ctgccgctgc gtatggccga
     1861 gtttggtagc tgccaccgta acgagccgtc aggttcgctg catggcctga tgcgcgtgcg
     1921 tggttttacc caggatgacg cgcatatctt ctgtactgaa gaacaaattc gcgatgaagt
     1981 taacggatgt atccgtttag tctatgatat gtacagcact tttggcttcg agaagatcgt
     2041 cgtcaaactc tccactcgtc ctgaaaaacg tattggcagc gacgaaatgt gggatcgtgc
     2101 tgaggcggac ctggcggttg cgctggaaga aaacaacatc ccgtttgaat atcaactggg
     2161 tgaaggcgct ttctacggtc cgaaaattga atttaccctg tatgactgcc tcgatcgtgc
     2221 atggcagtgc ggtacagtac agctggactt ctccttgccg tctcgtctga gcgcctccta
     2281 tgtgggcgaa gacaacgagc gtaaggttcc ggtaatgatt caccgcgcaa ttctggggtc
     2341 gatggaacgt ttcatcggta tcctgaccga agaattcgct ggtttcttcc cgacctggct
     2401 tgcgccggtt caggttgtta tcatgaatat taccgattca cagtctgatt acgttaacga
     2461 attgacgcaa aaactatcaa atgcgggcat tcgtgttaaa gcagacttga gaaatgagaa
     2521 gattggcttt aaaatccgcg agcacacttt gcgtcgcgtc ccatatatgc tggtctgtgg
     2581 tgataaagag gtggaatcag gcaaagttgc cgttcgcacc cgccgtggta aagacctggg
     2641 aagcatggac gtaaatgaag tgatcgagaa gctgcaacaa gagattcgca gccgcagtct
     2701 taaacaattg gaggaataag gtattaaagg cggaaaacga gttcaaacgg cgcgccctaa
     2761 ccgtatcaat ggcgaaattc gcgcccagga agttcgctta acaggtctgg aaggcgagca
     2821 gcttggtatt gtgagtctga gagaagctct ggagaaagca gaagaagccg gagtagactt
     2881 agtcgagatc agccctaacg ccgagccgcc ggtttgtcgt ataatggatt acggcaaatt
     2941 cctctatgaa aagagtaagt cttctaagga acagaagaaa aagcaaaaag ttatccaggt
     3001 taaggaaatt aaattccgtc ctggtacaga tgaaggcgac tatcaggtaa aactccgcag
     3061 cctgattcgc tttctcgaag agggtgataa agccaaaatc acgctgcgtt tccgcggtcg
     3121 tgagatggcg caccagcaaa tcggtatgga agtgcttaat cgcgtgaaag acgatttgca
     3181 agaactggca gtggtcgaat ccttcccaac gaagatcgaa ggccgccaga tgatcatggt
     3241 gctcgctcct aagaagaaac agtaaggcct tcaagtagca ctgtctgtgg agcctttggg
     3301 cttcacaggc attgttcgcc tttgtttcgt ttattaacaa tgcgaagtgg aagttattaa
     3361 aatgccaaaa attaagaccg tacgcggtgc tgctaagcgc ttcaaaaaaa ccggtaaagg
     3421 tggttttaag cacaagcacg ctaacctgcg tcacattctg accaaaaaag cgaccaaacg
     3481 taaacgtcac ctgcgtccga aagccatggt ttccaaaggc gatctgggcc tggtaatcgc
     3541 gtgcctgccg tacgcataag ccgttaacgt ttt
//
LOCUS       DAHHTG010000243         3502 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.249, whole genome shotgun sequence.
ACCESSION   DAHHTG010000243 DAHHTG010000000
VERSION     DAHHTG010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3502)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3502)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3502
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.249"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            <1..1874
                     /gene="sat"
                     /locus_tag="NBI74_004516"
     CDS             <1..1874
                     /gene="sat"
                     /locus_tag="NBI74_004516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001034089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="serine protease autotransporter toxin Sat"
                     /protein_id="HCD1934731.1"
                     /translation="NLAGKNITNNGFDFKQTISTNLSIGETKFTGGITAHNSQIAIGD
                     QAVVTLNGATFLNNTPISIDKGAKVIAQNSMFTTKGIDISGELTMMGIPEQNSKTVTP
                     GLHYAADGFRLSGGNANFIARNMASVTGNIYADDAATITLGQPETETPTISSAYQAWA
                     ETLLYGFDTAYRGAITAPKATVSMNNAIWHLNSQSSINRLETKDSMVRFTGDNGKFTT
                     LTVDNLTIDDSAFVLRANLAQADQLVVNKSLSGKNNLLLVDFIEKNGNSNGLNIDLVS
                     APKGTAVDVFKATTRSIGFSDVTPVIEQKNDTDKATWTLIGYKSVANADAAKKATLLM
                     SGGYKAFLAEVNNLNKRMGDLRDINGESGAWARIMSGTGSAGGGFSDNYTHVQVGADN
                     KHELDGLDLFTGVTMTYTDSHAGSDAFSGETKSVGAGLYASAMFESGAYIDLIGKYVH
                     HDNEYTTTFAGLGTRDYSSHSWYAGAEVGYRYHVTDSAWIEPQAELVYGAVSGKQFSW
                     KDQGMNLTMKDKDFNPLIGRTGVDVGKSFSGKDWKVTARAGLGYQFDLFANGETVLRD
                     ASGEKRIKGEKDGRMLMNVGLNAEIRDNLRFGLEFEKSAFGKYNVDNAINANFRYSF"
     gene            2146..2406
                     /locus_tag="NBI74_004517"
                     /pseudo
     CDS             2146..2406
                     /locus_tag="NBI74_004517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611998.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            2471..3013
                     /locus_tag="NBI74_004518"
                     /pseudo
     CDS             2471..3013
                     /locus_tag="NBI74_004518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414790.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30-like element IS30 family transposase"
     gene            complement(3048..>3502)
                     /locus_tag="NBI74_004519"
     CDS             complement(3048..>3502)
                     /locus_tag="NBI74_004519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HCD1934732.1"
                     /translation="TGKRWAYLAVVLDLFARKPVGWAMSFSPDSRLTMKALEMAWETR
                     GKPVGVMFHSDQGSHYTSRQFRQLLWRYRIRQSMSRRGNCWDNSPMERFFRSLKNEWV
                     PATGYVSFSDAAHAITDYIVGYYSALRPHEYNGGLPPNESENRYWKKL"
BASE COUNT          992 a          811 c          832 g          867 t
ORIGIN      
        1 ataatcttgc agggaaaaat attactaata atggttttga cttcaaacaa actatcagta
       61 ctaatctatc cataggagaa actaaattta caggtggcat cactgcacat aacagccaaa
      121 tagccatagg tgatcaagct gtagttacac ttaatggtgc aacctttctg aataatactc
      181 ctataagtat agataaagga gcaaaagtta tagcacaaaa ttccatgttc acaacaaaag
      241 gtattgatat ctccggtgag ctgactatga tgggaatccc tgaacagaat agtaaaactg
      301 taacgccggg tctccactac gctgctgatg gattcaggct gagtggtgga aatgcaaatt
      361 tcattgccag aaatatggca tctgtcaccg gaaatattta tgctgatgat gcagcaacca
      421 ttactctggg acagcctgaa actgaaacac cgactatatc gtctgcttat caggcatggg
      481 cagagactct tttgtatggc tttgataccg cctatcgagg cgcaataaca gcccccaaag
      541 ctacagttag catgaataat gcgatctggc atctaaatag ccagtcatca attaatcgtc
      601 tagaaacaaa agacagtatg gtgcgtttta ctggtgataa tgggaagttt acaaccctta
      661 cagtggacaa ccttactata gatgacagtg catttgtgct gcgtgcaaat ctggcccaag
      721 cagatcagct tgttgtcaat aaatcgttgt ctggtaaaaa caaccttctg ttagtcgact
      781 tcattgagaa aaatggaaac agcaacggac tgaatatcga tctggtcagc gcaccaaaag
      841 gaactgcagt agatgtcttt aaagctacga ctcggagtat tggcttcagt gatgtaacac
      901 cggttatcga gcaaaagaac gatacagaca aagcaacatg gactctgatc ggctataaat
      961 ctgtggccaa cgccgatgcg gctaaaaagg caacattact gatgtcaggc ggctataaag
     1021 ccttccttgc tgaggtcaac aaccttaaca aacgtatggg tgatctgcgt gacattaacg
     1081 gtgagtccgg tgcatgggcc cgaatcatga gcggaaccgg gtctgccggc ggtggattca
     1141 gtgacaacta cacccacgtt caggtcggtg cggataacaa acatgaactc gatggccttg
     1201 acctcttcac cggggtgacc atgacctata ccgacagcca tgcaggcagt gatgccttca
     1261 gtggtgaaac gaagtctgtg ggtgccggtc tctatgcctc tgccatgttt gagtccggag
     1321 catatatcga cctcatcggt aagtacgttc accatgacaa cgagtatacc acaactttcg
     1381 ccggccttgg caccagagac tacagctccc actcctggta tgccggtgcg gaagtcggtt
     1441 accgttacca tgtaactgac tctgcatgga ttgagccgca ggcggaactt gtttacggtg
     1501 ctgtatccgg gaaacagttc tcctggaagg accagggaat gaacctcacc atgaaggata
     1561 aggactttaa tccgctgatt gggcgtaccg gtgttgatgt gggtaaatcc ttctcgggta
     1621 aggactggaa agtcacagcc cgcgccggcc ttggctacca gtttgacctg tttgccaacg
     1681 gtgaaaccgt actgcgtgat gcgtccggtg agaaacgtat caaaggtgaa aaagacggtc
     1741 gtatgctcat gaatgttggt ctcaacgctg aaattcgcga taatcttcgc ttcggtcttg
     1801 agtttgagaa atcggcattt ggtaaataca acgtggataa cgcgatcaac gccaacttcc
     1861 gttactcttt ctgataacag cccgggccgc gtttgcggcc cttcttctac cggagagaat
     1921 atgtattacc ctgtgacaga ctatatcgct cttgctctca ttattagctt tctttttctg
     1981 acattattta tctgcctgtt atgccttaaa catgagcgaa taaaaaaaga aactatcagg
     2041 caaaaaaatg cacatattct ggatcatggc tggaatgcaa ctgaggtagc ctgagtttaa
     2101 cggacactcc ttcctgaaat agaatggcat cagaaggagc taataatgag cagaaaaacc
     2161 caacgttact ctaaagagtt caaagccgaa gctgtcagaa cggttcttga aaatcaactt
     2221 tcgatcagtg aaggcgcttc ccgattatct cttcctgaag gcactttagg acaatgggtt
     2281 accgccgcca gaaaagggct cggtactcct ggttcccgca cggtggctga actggaatct
     2341 gaaattctgc aactgcgtaa ggcgttaaat gaagctcgcc ttgagcgaga tatattaaaa
     2401 aaagcaactg tagattcaat tggtcaacgc aacagttatg tgaaaacatg gggttgcgga
     2461 ggttttttga atgagacgaa catttacagc agaggaaaaa gcctctgttt ttgaactatg
     2521 gaagaacgga acaggcttca gtgaaatagc gaatatcctg ggttcaaaac ccggaacgat
     2581 cttcactatg ttaagggata ctggcggcat aaaaccccat gagcgtaagc gggctgtagc
     2641 tcacctgaca ctgtctgagc gcgaggagat acgagctggt ttgtcagcca aaatgagcat
     2701 tcgtgcgata gctactgcgc tgaatcgcag tccttcgacg atctcacgtg aagttcagcg
     2761 taatcggggc agacgctatt acaaagctgt tgatgctaat aaccgagcca acagaatggc
     2821 gaaaaggcca aaaccgtgct tactggatca aaatttacca ttgcgaaagc ttgttctgga
     2881 aaagctggag atgaaatggt ctccagagca aatatcagga tggttaaggc gaacaaaacc
     2941 acgtcaaaaa acgctgcgaa tatcacctga gacaatttat aaaacgctgt actttcgtag
     3001 ccgtgaagcg ctacacctga agtggtcaac aaaaactggc caccgagtta gagttttttc
     3061 cagtatcgat tttccgattc gtttggtggt aacccaccat tatattcgtg cggtcttagt
     3121 gcgctgtaat atccaacgat atagtccgtt attgcgtgag ctgcatcgct gaagcttaca
     3181 tagcccgtcg ccggcaccca ttcgttcttc agactcctga agaagcgctc cattgggctg
     3241 ttatcccagc agtttccacg ccgactcata ctctgcctga tccggtatct ccacagtaac
     3301 tgccggaact gcctgctcgt ataatgactg ccttgatcgc tgtggaacat caccccgacg
     3361 ggcttaccac gggtttccca tgccatttcc agtgctttca tggtaagcct gctgtccggc
     3421 gagaacgaca tggcccagcc cactggtttt cttgcgaaca ggtcgagaac aacggcgagg
     3481 tacgcccagc gcttacccgt cc
//
LOCUS       DAHHTG010000244         3500 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.250, whole genome shotgun sequence.
ACCESSION   DAHHTG010000244 DAHHTG010000000
VERSION     DAHHTG010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3500)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3500)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3500
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.250"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            complement(61..657)
                     /locus_tag="NBI74_004520"
     CDS             complement(61..657)
                     /locus_tag="NBI74_004520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001683249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HCD1934733.1"
                     /translation="MNNTDLIHLIKHFMRNEHKAVEEVIDSPLSEVANLIKVLQSCQG
                     KVVFIGVGKSGIIARKLAATFASTGTPSFFVHGTEAVHGDLGMVAKDDVVILISNSGE
                     TAEILATLPSLKKMGNYLISFTRSHHSSLAISCDLSVEIPVKSEADNLGLAPSCSSTV
                     VLVVGDAVALALSELKKFTRADFGLYHPGGALGIKANS"
     gene            complement(660..1835)
                     /locus_tag="NBI74_004521"
     CDS             complement(660..1835)
                     /locus_tag="NBI74_004521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000382418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PatB family C-S lyase"
                     /protein_id="HCD1934734.1"
                     /translation="MDVFNTPVSRKGTYCTQWDFCEDRFGVKDVLPFSISDMDLPIPD
                     AITRALKKRLEHPILGYSRWQHGEYLNAIVNWYYQQYQTDIKPEWITYSPSVMHSIAK
                     AIELLTSHGDNILVFTPVYNAFFDVIKHSERNILTASLIIKNEGDYAINWQDFDLKIK
                     SAKMVLLCNPHNPTGTVWSEEELHKIAASCTRHNVWLCSDEIHSDFVFNRSFTSALKI
                     KKEKVVVFNSISKTFNVPALTGSYMISTDDNFNHKFRTISRYRDFVNSPSVLNIIATI
                     IAYNECEGWLKSLKAHIASNIQFTQQYLNENIPELIVRPADGCYFSWIDCSAIGYPFD
                     EFYSRLIHEGKVGIMAGHVYGTEGEGYLRLNLACGREKLYMGLTRLVSVIKNINQGE"
     gene            complement(1835..3415)
                     /locus_tag="NBI74_004522"
     CDS             complement(1835..3415)
                     /locus_tag="NBI74_004522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000810561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HCD1934735.1"
                     /translation="MKQKKAWSFFQSLGKAFMYPIALLSVCGMMLGLGSGLASDDMAK
                     LIPFLAIPIIKTILDFIVSLGLFAFVNLPVLFAIAIPLGLLKDKEDKAYGAFSGLIGF
                     MAMHLGTNFYLKQHDLLVVADQMSTHGQTIILGIQSYNTSVLGGIVAGLLVASMYKKI
                     VNLRIPESLGFYSGPRLVPIITLIVMSGFGLIIPFIWPPFFNLFMLIGHWISTSGPVG
                     YFFYAVAERVTIPFGLNHLVTSVFRFTPIGGSAVIGGEEYYGTLNMFMAYVKENAVIP
                     LDLAGKMEQGKLMIQYGLAGAALAMYRTAHAQNRKAIKALLISGVLTVIIGGVSEPIE
                     FLFLFVSPLLFVFHAFMNGFANMVLPYMGVKMGFTGDLIQFISFGVLRGTRTGWPIAV
                     CVEVAYFFIYYFVFRWTILKFNLMTVGREESSPVTLNAHEDTAIADIPTPDKSELQAA
                     EQMVKALGGKENIKSLDNCVTRLRLTIADMGLIDEVAIKIAGGIAVVKLDQNTLQVII
                     GTKVIALRRDMDNYMGIY"
BASE COUNT         1092 a          795 c          645 g          968 t
ORIGIN      
        1 acttcaaaga acgccacccc aaaagatagc gtgaccggtt actgtattta tattttgaaa
       61 ttatgaattt gctttaatgc cgagtgcacc tcctggatga tataagccga aatcagcacg
      121 agtaaatttt ttcaattcag agagtgcaag cgccaccgca tcaccaacaa ccagaacaac
      181 agtcgacgag catgacggtg ctaaacctaa attatccgcc tcacttttga cggggatttc
      241 aacagataaa tcacaactta tcgcaagcga tgagtgatga ctgcgtgtaa aggagatcaa
      301 ataattaccc atttttttca gactaggcag tgtcgccagg atttccgccg tctcaccact
      361 gtttgaaata agaataacaa catcgtcttt cgccaccatt ccaaggtcgc cgtgtaccgc
      421 ttccgtaccg tgaacaaaaa acgagggagt tccggtgctg gcaaatgttg cggcgagttt
      481 cctggcaata ataccggact taccaacacc aataaaaaca acttttccct gacaagattg
      541 taatacttta attaaatttg cgacctccga aagaggggaa tcaataactt cttcaactgc
      601 tttatgttcg ttacgcataa aatgtttaat gagatggata agatccgtgt tattcatttt
      661 tattctccct gattaatatt tttaattaca ctcacaagac gcgtaagccc catatataat
      721 ttttctcgac cgcaggccag gttcagacga aggtagccct ccccctccgt tccatagaca
      781 tgccccgcca taatgcccac tttgccttca tgaattaatc gactgtagaa ttcatcaaaa
      841 ggataaccaa tagcagaaca atctatccag gaaaaatagc aaccatcagc tggccttacg
      901 atcaattctg gaatattttc attcagatac tgctgggtga attgaatgtt agaggcaata
      961 tgtgccttta atgactttaa ccatccctca cattcgttat aggcaataat agttgcaatt
     1021 atattcagta ctgatgggga gtttacaaaa tcgcgatacc tcgatatcgt tctgaactta
     1081 tgattaaaat tatcgtcagt tgaaatcata tacgatcctg ttagagcagg aacattaaat
     1141 gttttcgata tcgagttaaa tactactact ttttcttttt ttattttaag cgcggatgta
     1201 aagctacgat taaaaacaaa atcactatgg atttcatctg aacataacca gacattatgc
     1261 cttgtacagc ttgcagcgat tttatgcagt tcttcttccg accagactgt tcctgtcggg
     1321 ttatgaggat tacataataa caccatcttt gcgcttttaa tcttaagatc aaaatcttgc
     1381 cagttgatcg catagtcccc ctcatttttt ataatcaaag aggctgtcag aatatttcgc
     1441 tcgctatgtt taattacatc aaaaaatgca ttatagacag gtgtaaaaac gagaatatta
     1501 tcaccatgcg atgtcaataa ctctattgct tttgcaatag agtgcatcac actggggcta
     1561 taggttatcc attcaggttt aatatctgtt tgatattgtt gataatacca gtttactatc
     1621 gcattcagat attcaccatg ttgccaacga ctgtacccta aaatcgggtg ttccaggcgt
     1681 ttttttaatg ctctggtgat agcgtctggt atcggaagat ccatatctga aatagagaat
     1741 ggtagtacat cttttacacc aaatctgtcc tcgcaaaagt cccattgggt acaatatgtt
     1801 cctttgcggg aaacaggagt attaaaaaca tccatcagta tatccccata tagttatcca
     1861 tatcccgacg cagggcgatg actttagtgc cgataatgac ttgtagggta ttttgatcaa
     1921 gtttaacaac cgcaatcccg ccagctattt ttattgcaac ttcgtcaatc aatcccatat
     1981 ctgcgattgt taaacgtaaa cgagttacgc aattatccag tgacttaata ttctctttac
     2041 caccaagtgc cttaaccatc tgctccgccg cttgcagctc tgatttatca ggagttggga
     2101 tatccgctat agccgtatct tcgtgagcgt tcagcgtgac aggactggac tcttcacggc
     2161 ctacggtcat caggttaaat ttaagaatgg tccaacggaa cacaaagtaa taaatgaaga
     2221 aataggccac ttcgacacac accgcgatcg gccaacctgt tcttgtgcca cgcaatacgc
     2281 caaagctaat aaattgaatc agatcgccag taaatcccat cttcaccccc atatatggta
     2341 gaaccatgtt agcgaatcca ttcataaagg catggaagac aaacagcagt ggactgacaa
     2401 ataagaacag aaactcaatc ggttcgctga cgccgccaat aatcaccgta agcaccccgg
     2461 aaataagcaa tgctttgata gcctttctgt tttgagcatg agcagtgcga tacatcgcca
     2521 gcgcggcacc agccagacca tactgaatca tcagtttgcc ctgctccatt ttccccgcca
     2581 aatccagcgg aatgaccgca ttctctttga cgtacgccat aaacatgttc agggtgccgt
     2641 aatattcttc gccgccaatc acagccgaac cgccgattgg cgtaaagcgg aaaactgacg
     2701 tcaccagatg gtttaagcca aaaggaatcg tcacgcgttc ggcaactgca tagaagaaat
     2761 aaccaacagg accggaagtt gaaatccagt gtccaatgag catgaaaaga ttgaaaaacg
     2821 gcggccagat aaaaggaatg atcagaccaa atccactcat cacaatcagt gtaatgatag
     2881 gcaccagacg tgggccgcta taaaaaccta acgattcagg aatgcgtaaa ttaacgatct
     2941 ttttatacat gctggcgact aataacccag caacaattcc ccccaacacg ctggtattgt
     3001 aggactggat ccccagaatg atggtttgcc catgtgtcga catttggtca gcaacgacca
     3061 ataagtcgtg ctgtttaaga taaaagttcg ttcccagatg catcgccata aaaccaatta
     3121 agccagaaaa agcaccatag gctttatcct ctttatcttt taataatcct aagggaatcg
     3181 ctatcgcaaa caatacaggt aaattaacaa aggcaaacaa accaagacta acaatgaaat
     3241 caagtatggt tttaattatt ggaatagcca gaaatggaat taactttgcc atatcatcac
     3301 tggctaaacc acttcccagc cctagcatca tgccacatac acttagcaga gcaatgggat
     3361 acataaatgc cttccccagg ctctgaaaaa aactccaggc tttcttttgt ttcatgtggg
     3421 ttatctcata taaatgttat atataattag tccattaata ctttggtacg aatagagaga
     3481 tatagttttt cttctaaaat
//
LOCUS       DAHHTG010000245         3486 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.251, whole genome shotgun sequence.
ACCESSION   DAHHTG010000245 DAHHTG010000000
VERSION     DAHHTG010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3486)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3486)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3486
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.251"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            complement(<1..684)
                     /locus_tag="NBI74_004523"
     CDS             complement(<1..684)
                     /locus_tag="NBI74_004523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HCD1934736.1"
                     /translation="MKNSKAFYRSTLATAIVMALSAPAFAADNAVSTTPVTLDKDKTT
                     LEQDVVISGTTQITAATINAKDKDLNVTLGGHDITATSTVDQGFVEGVKVSGDKNVVI
                     NATGSTITAQGEGTYVRTAMVIDSTGDVVVNGGNFVAKNEKSSATGISLEATTGNNLT
                     LNGTTINAQGNKSSSNGSTAIFAQKGSVLNGFNGDATDNITLAGSNIINGGIETIVIA
                     KENKGTHTVN"
     gene            complement(726..1487)
                     /gene="hyxA"
                     /locus_tag="NBI74_004524"
     CDS             complement(726..1487)
                     /gene="hyxA"
                     /locus_tag="NBI74_004524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein HyxA"
                     /protein_id="HCD1934737.1"
                     /translation="MRFVLFCPSGIVPAQFAALSTGSVGNVTCIRTEEELRNKLRHRP
                     QSVVISAGRPAECAEMWFRFYRDHSFVVVLCVAPFFLPPDVSISGVLKNLRLLKPGMS
                     VERVISIANASGGFSGLKHAEILPVMDSYSVFMKEVNNRTKTIVMSVRFPEKQKKVLS
                     LLLAGHSWEYSAQFLKTGIRQIWLTEQSLKKRWGIPDSMSLREALLLSSNNFHGDGNA
                     LETTNAMTLRENGNTNRYSVVVNAGTQALSLHKYK"
     gene            complement(1641..2435)
                     /gene="hyxR"
                     /locus_tag="NBI74_004525"
     CDS             complement(1641..2435)
                     /gene="hyxR"
                     /locus_tag="NBI74_004525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001304859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR family transcriptional regulator HyxR"
                     /protein_id="HCD1934738.1"
                     /translation="MKKPLVIISACQFTRLALESLIPTDRYIVRVYSNVTTEVEFVLK
                     TSCGYLLADIPSLPSGELVLLACLARLRSDAGQWQVCLTGDSDWFDNAPAASLYNGFP
                     RIGTMLTASRYQNQIIRWLLRPLPGDMSDFWLLTARELSVLKILMQGMSFSEIARYEQ
                     RSSKTLHAIATRALMKLGLRTLSDFRLLYTGCGDSRVKRNIRLHAKYIGQHSAHRLTQ
                     YVQNMVSGILYPEKVAVGESINSDAISGKVLSGQRKNISKKRPGLL"
     gene            complement(2765..3328)
                     /gene="ipbA"
                     /locus_tag="NBI74_004526"
                     /gene_synonym="fimX"
     CDS             complement(2765..3328)
                     /gene="ipbA"
                     /locus_tag="NBI74_004526"
                     /gene_synonym="fimX"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_060710320.1"
                     /note="IpbA is also known as HbiF and FimX; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type DNA invertase IpbA"
                     /protein_id="HCD1934739.1"
                     /translation="MTRKYLTQDEVYRLMDAAQSMSFPERNRCLIMMAFIHGFRASEL
                     LDLRLSDIDASGKQLNIRRIKNGFSTTHPLLPDEYNLIKLWLKQRKLIENGVEGDWLF
                     LSRKRRPISRQHFFSIIREAGKRAGLAVKAHPHMLRHACGFALADNGVDTRLLQDYLG
                     HRNIQHTVRYTASNAARFKGVWKKKPR"
BASE COUNT          950 a          799 c          719 g         1018 t
ORIGIN      
        1 gttgactgta tgagttccct tattctcctt ggcaataact attgtttcaa tcccgccatt
       61 aataatattt gagccagcaa gggtaatgtt gtcggttgca tcaccgttaa aaccattcaa
      121 tacgctaccc ttttgagcaa aaattgccgt agagccgtta ctggaactct tattaccttg
      181 agcatttatg gttgtgccat tgagcgttaa attatttccc gtggtcgctt ccagagatat
      241 acctgtcgca ctactttttt cattttttgc aacaaagtta ccgccattaa caacaacatc
      301 gccagttgaa tcaatgacca ttgcagttcg gacataggta ccttcacctt gagctgtgat
      361 ggtggagcct gtagcattaa tcacaacatt tttgtcaccg ctaactttta caccttcaac
      421 gaaaccttgg tctaccgttg atgtggcggt aatatcatga ccacccaaag taacattaag
      481 gtctttatct ttcgcattaa ttgttgcggc tgtaatctgt gtagtaccac taataacaac
      541 atcttgttcc agagttgtct tatctttatc caatgtaacc ggtgtagttg ataccgcgtt
      601 atcagcagcg aatgctggtg cagaaagagc cataacaata gctgtcgcta atgtgctgcg
      661 gtaaaatgcc ttactgtttt tcatgaagaa attctccatt ctggtaacta atttcaattt
      721 cccacttatt tatatttatg cagagataaa gcctgcgtcc ctgcatttac caccacggaa
      781 tacctgttgg tatttccgtt ttcacgcaat gtcatggcat tcgttgtttc caatgcattt
      841 ccatcaccgt gaaaattatt gctgctcagc agcagggctt cccgcaatga catgctgtcc
      901 ggtattcccc aacgcttttt tagcgactgc tcggtaagcc atatctgtct tatccccgtt
      961 ttgagaaatt gcgcactgta ttcccagctg tgtccggcaa gaagcaacga caacaccttc
     1021 ttttgctttt ccgggaagcg tactgacatg acgattgtct ttgtgcggtt attcacctct
     1081 ttcataaaga cagagtaact gtccataact ggcaaaattt ctgcatgttt cagccctgaa
     1141 aacccaccag aggcatttgc aatactgatg acacgctcta ctgacattcc tggtttcagt
     1201 aacctcaggt ttttgagaac gccagaaata ctcacatccg gtggcagaaa aaagggcgcg
     1261 acgcacaaca ccaccacgaa tgagtgatcc cgataaaatc ggaaccacat ttcagcgcat
     1321 tcggcaggtc gccctgccga tatcacgaca ctctgtggcc tgtgccgcaa tttattgcgt
     1381 aactcctctt ctgttctgat gcaggtaaca ttgccgacag aaccggttga tagagctgcg
     1441 aactgcgctg gtacaattcc tgagggacag aaaagaacga aacgcatcct tccctcacag
     1501 atgtcagtac gccagcacgc tgtacaggct gtacagcatg cctcccaatc ctgcagccca
     1561 gaacagtaga aatattttgg ctcctacact gtagcgagtc cgtgtgcgcc gcagtgggtg
     1621 gtagtaacaa cgctgctgtg tcataacaat cctggtcttt ttttagatat attttttctt
     1681 tgtccggaaa gtacttttcc ggaaatggcg tcagaattta ttgattcacc aacggcaact
     1741 ttctctggat aaaggattcc ggataccatg ttctgcacat actgagtgag tcgatgagcg
     1801 gaatgttgtc cgatatattt tgcatgtagc cgtatattcc gtttcactct gctgtcaccg
     1861 catcccgtat agagcagcct gaaatcgctc agtgttctca gtccaagttt cattaaagcc
     1921 cgtgtggcaa tcgcatgcag ggtctttgaa ctgcgttgtt cataacgggc aatctcactg
     1981 aaactcatgc cctgcatcag aatcttaagc acgctcaact cccgtgcagt caggagccag
     2041 aaatcgctca tatcgccggg caaaggacgc agtaaccagc ggataatttg attctgataa
     2101 cgactcgctg tcagcatggt gccgatgcgt gggaagccgt tatacagact tgcagcagga
     2161 gcattatcga accagtcgga gtcaccggtc aggcagacct gccattgccc ggcatcactc
     2221 cgcaaacggg cgagacaagc cagaagaacc aactcccccg aaggaagcga agggatatca
     2281 gctaacaggt agccacagga ggtctttaaa acgaattcaa cctctgtagt gacgttgctg
     2341 tacacccgca cgatatacct gtccgttggg atgagagact ccagtgccag cctggtgaac
     2401 tgacaggcac tgataataac cagaggcttc ttcattcaga ctcgtccacg ccattgctac
     2461 aaaacctgac attttgattc aaaatgtcag gtttggttta atagcctgaa tatttaattt
     2521 tcttggaata taaatctaat ttgtttgatt tttattgttt ttaattttgt gttatttaac
     2581 aatatgagcg aaagttaatc actggtatga cctttgtgat taatcatttc aaatcatggg
     2641 gttattttac tttagctaga aatatcaaca tgttatgtgt tttatttaat tgaatgggct
     2701 gtttttttat ggaagaattg aggaaaacgt aggaatctga cattttgaat cagaaggtac
     2761 tgttttaacg aggctttttt ttccacaccc ctttaaaacg agcagcatta ctggctgtgt
     2821 atctgacagt atgctgaata tttcgatgac ccagataatc ctgaagtagt cgggtatcga
     2881 caccattgtc agccagagca aaaccacagg catgacgtaa catatgagga tgtgctttta
     2941 cagctaatcc tgcacgtttt ccagcctcac gaatgataga aaaaaaatgt tgtctgctga
     3001 tagggcggcg tttccgggag agaaaaagcc agtctccttc aactccgttt tcaattaact
     3061 tcctctgctt aagccacaac ttgatcaaat tatattcatc aggaagaaga ggatgtgttg
     3121 tcgagaaacc atttttaata cgccgaatat taagttgttt tccagatgca tcaatatcgg
     3181 ataaacgtaa atcaagaagt tcactggccc taaaaccgtg aatgaaagcc atcataatta
     3241 gacagcgatt tctttcagga aaggacatgc tctgggctgc atccatcagc ctgtagactt
     3301 catcctgtgt gagatatttt ctcgtcatac gccaccgtgt acagttgtaa aaaaagagag
     3361 gcacacccag ataagtatgc ctgcaatcag aacgttatcg atgtaatctc attatctcat
     3421 taataagatt ttttgaatag tagttctacg caacagatta aatatgaact atttattgtg
     3481 cagctt
//
LOCUS       DAHHTG010000246         3483 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.252, whole genome shotgun sequence.
ACCESSION   DAHHTG010000246 DAHHTG010000000
VERSION     DAHHTG010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3483)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3483)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3483
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.252"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            166..1686
                     /locus_tag="NBI74_004527"
     CDS             166..1686
                     /locus_tag="NBI74_004527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HCD1934740.1"
                     /translation="MSLSIVHTRAALGVNAPPITVEVHISKGLPGLTMVGLPETTVKE
                     ARDRVRSAIINSGYEYPAKKITINLAPADLPKEGGRYDLPIAIALLAASEQLTANKLD
                     EYELVGELALTGALRGVPGAISSATEAIKSGRKIIVAKDNEDEVGLINGEGCLIADHL
                     QAVCAFLEGKHALEHPKPTNAVSRALQHDLSDVVGQEQGKRGLEITAAGGHNLLLIGP
                     PGTGKTMLASRINGLLPDLSNEEALESAAILSLVNAESVQKQWRQRPFRSPHHSASLT
                     AMVGGGAIPGPGEISLAHNGVLFLDELPEFERRTLDALREPIESGQIHLSRTRAKITY
                     PARFQLVAAMNPSPTGHYQGNHNRCTPEQTLRYLNRLSGPFLDRFDLSLEIPLPPPGI
                     LSKTVVPGENSTTVKQRVMAARERQFKRQNKLNAWLDNPEIRQFCKLESEDAQWLEET
                     LIHLGLSIRAWQRLLKVARTIADIDQSDIITRQHLQEAVSYRAIDRLLIHLQKLLT"
     gene            complement(1711..2049)
                     /gene="maoP"
                     /locus_tag="NBI74_004528"
     CDS             complement(1711..2049)
                     /gene="maoP"
                     /locus_tag="NBI74_004528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ori organization protein MaoP"
                     /protein_id="HCD1934741.1"
                     /translation="MAESFTTTNRYFDNKHYPRGFSRHGDFTIKEAQLLERHGYAFNE
                     LDLGKREPVTEEEKLFVAVCRGEREPVTEAERVWSKYMTRIKRPKRFHTLSGGKPQVE
                     GAEDYTDSDD"
     gene            2168..3007
                     /gene="hdfR"
                     /locus_tag="NBI74_004529"
     CDS             2168..3007
                     /gene="hdfR"
                     /locus_tag="NBI74_004529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator HdfR"
                     /protein_id="HCD1934742.1"
                     /translation="MDTELLKTFLEVSRTRHFGRAAESLYLTQSAVSFRIRQLENQLG
                     VNLFTRHRNNIRLTAAGEKLLPYAETLMSTWQAARKEVAHTSRHNEFSIGASASLWEC
                     MLNQWLGRLYQNQDVHTGLQFEARIAQRQSLVKQLHERQLDLLITTEAPKMDEFCSQL
                     LGYFTLALYTSAPSKLKGDLNYLRLEWGPDFQQHEAGLIGADEVPILTTSSAELAQQQ
                     IAMLNGCTWLPVSWARKKGGLHTVVDSTTLSRPLYAIWLQNSDKNALIRDLLKINVLD
                     EVY"
     gene            complement(3103..3178)
                     /locus_tag="NBI74_004530"
     tRNA            complement(3103..3178)
                     /locus_tag="NBI74_004530"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3143..3145),aa:Trp,seq:cca)
     gene            complement(3187..3263)
                     /locus_tag="NBI74_004531"
     tRNA            complement(3187..3263)
                     /locus_tag="NBI74_004531"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3227..3229),aa:Asp,seq:gtc)
     gene            complement(3318..3433)
                     /gene="rrf"
                     /locus_tag="NBI74_004532"
     rRNA            complement(3318..3433)
                     /gene="rrf"
                     /locus_tag="NBI74_004532"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT          918 a          870 c          854 g          841 t
ORIGIN      
        1 tcgtagttca tcctttgccc gctgcatttt tgcgagcatc attccaccgc cagaacaact
       61 caacttcact tgcacaaata aacaggaatc agccactaaa attcacgaat tagcgggcta
      121 cagcagcgat atcttatgca cataatggtg actcaaggag ggcttatgtc actgtcaatt
      181 gttcataccc gcgcagccct gggagtaaat gcgcccccaa tcactgttga ggtacatatc
      241 agtaaaggtc taccaggctt aacgatggtg ggcttaccag aaacaacggt aaaagaagct
      301 cgcgatcgcg tgcgcagcgc cattatcaat agcggatatg aatatccggc gaaaaaaatc
      361 accatcaacc ttgctccagc cgatctgcca aaagaagggg gacgatatga tttgcctatc
      421 gctattgcgt tgctggcggc ctcagaacag cttacagcca ataagttaga tgaatatgaa
      481 ttagtcggag aactggcgct tacaggcgct ctgcgtggcg ttcccggcgc aatctccagt
      541 gcaactgaag ctattaagtc gggcagaaaa attatcgtcg cgaaagataa cgaagatgaa
      601 gtggggctaa ttaacggtga aggatgcctg atagccgatc atctgcaggc tgtctgcgcg
      661 tttctggaag gtaagcacgc tctcgaacac ccgaaaccaa ctaatgcagt atcccgggcg
      721 ctacaacatg atctcagtga tgttgtcggt caggagcaag gaaagcgagg actggaaatt
      781 accgccgctg gcgggcacaa ccttttactg attggaccgc cgggaacagg taaaacaatg
      841 ctcgccagcc gtattaatgg ccttttgcca gatttaagca atgaagaggc actggagagc
      901 gctgcgatat taagtctggt aaatgctgaa tcagtacaaa aacaatggcg gcaacgcccg
      961 ttccgctcac ctcatcacag tgcgtcgtta actgcgatgg tgggcggtgg tgcaattcca
     1021 gggccaggtg aaatttcgct ggcgcataac ggcgtgcttt ttcttgatga gctacctgaa
     1081 tttgaacggc gtacactgga tgccttgcga gagccgattg aatccgggca gatccatctt
     1141 tcacgcacac gagcaaaaat aacctatcca gcccgtttcc agcttgtcgc ggcgatgaat
     1201 cccagcccta ccggacatta tcagggaaac cataaccgct gcacaccaga acagacatta
     1261 cgttatctca accggctctc ggggcccttt ctcgaccgct tcgatctctc actggagatc
     1321 ccattaccgc ctcccggcat tttgagtaaa acggtagtgc cgggagaaaa cagcaccacc
     1381 gttaaacaac gtgtaatggc cgccagagag cgccaattta agcggcagaa taaactgaac
     1441 gcctggctgg ataatccgga aatacgccaa ttctgcaagc ttgagagcga agatgcgcag
     1501 tggctggaag aaacgctgat ccatctgggg ttatcgattc gtgcctggca gcggttattg
     1561 aaagttgcac gaaccattgc tgatattgat cagtctgaca ttatcacacg tcagcatttg
     1621 caggaggcag ttagctatcg tgcgattgac cgtttgctca tccatctgca aaaactactg
     1681 acataaaaaa agggcatttc gcccttttta ttaatcgtca gaatcggtgt agtcttcagc
     1741 accttcaacc tgcggtttac cgccggaaag ggtgtgaaaa cgttttggac gcttgatacg
     1801 cgtcatatac ttggaccaca cgcgttctgc ttctgtcact ggttcacgtt cgccacggca
     1861 tactgctacg aagagtttct cttcctcggt aaccggctcg cgtttgccaa gatccaactc
     1921 attgaaggca taaccatgac gctcaagcag ttgtgcctct ttgatggtga agtcaccatg
     1981 acgagagaat ccacgtggat aatgtttatt gtcgaaatat cgattagtcg tcgtaaagct
     2041 ttccgccatc ctgcacgctc ctaattcttt gaccgagcta gttatggcgc ggagtattag
     2101 ttacgcttga cagagtgtaa aacaaaacat ttaaatcata acgacaaata attttgcgga
     2161 gagcactgtg gatacggaat tgttaaaaac tttcctggaa gttagccgaa cgcgtcactt
     2221 tggtcgagcg gctgaatcgc tctatctgac ccaatcagcc gtgagctttc gaatcagaca
     2281 actggaaaat caactgggtg tgaatctttt cacccgccac agaaacaata tccgtttaac
     2341 cgctgccgga gaaaagcttc tgccttatgc agaaacgctc atgagcacct ggcaggccgc
     2401 ccgcaaggag gtggcgcata cctcacgaca taatgagttt tctatcggtg ccagcgcctc
     2461 gctgtgggag tgtatgctta atcagtggct gggacgcttg tatcaaaatc aggatgtcca
     2521 tacaggctta cagttcgaag cgcgaattgc ccaacggcag tctctggtaa aacagttgca
     2581 tgaacgccag cttgatcttc ttattacaac tgaagccccc aaaatggacg aattttgtag
     2641 tcagttgctg gggtatttca ctttagcgct ttataccagt gccccttcta aactaaaggg
     2701 agatcttaac tatctgcgac tagagtgggg gccagacttt caacagcatg aggcaggttt
     2761 gatcggtgct gacgaagttc ccattctgac aaccagttct gctgaactgg cacagcaaca
     2821 gattgcgatg cttaatggtt gcacctggct acccgtcagt tgggcacgta aaaaaggcgg
     2881 cctgcatacc gttgtcgata gcacaacact ttcacggccg ctttatgcca tatggctaca
     2941 aaatagcgat aaaaatgcgt tgattcgcga tctattgaaa attaacgtgc tggatgaagt
     3001 gtattaatct gaatggctgg caaggatgcc ggtagaagga tttacttcgg agagggttat
     3061 ttcagataaa aaaaatcctt agctttcgct aaggatgatt tctggcaggg gcggagagac
     3121 tcgaactccc aacacccggt tttggagacc ggtgctctac caattgaact acgcccctaa
     3181 ttagggtggc ggaacggacg ggactcgaac ccgcgacccc ctgcgtgaca ggcaggtatt
     3241 ctaaccgact gaactaccgc tccaccgaat tcttttacaa ccaccggttt tatgaccggc
     3301 ttactgctta atttgatgcc tggcagttcc ctactctcgc atggggagac cccacactac
     3361 catcggcgct acggcgtttc acttctgagt tcggcatggg gtcaggtggg accaccgcgc
     3421 taaggccgcc aggcaaattc tgttttatca acacgtcccg ttatgacctg ttgattcaat
     3481 ctg
//
LOCUS       DAHHTG010000247         3469 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.253, whole genome shotgun sequence.
ACCESSION   DAHHTG010000247 DAHHTG010000000
VERSION     DAHHTG010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3469)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3469)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3469
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.253"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            99..1571
                     /locus_tag="NBI74_004533"
     CDS             99..1571
                     /locus_tag="NBI74_004533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000376547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCD1934743.1"
                     /translation="MDSCIQKKQVWYKHLSPRQWKIFGAAWTGYLLDGFDFVLISLVL
                     TEVQHEFGLTTIEAASLISAAFISRWFGGLAIGALSDKMGRRMAMVLSIVLFSLGTLA
                     CGLAPGYAVMFIARIVIGLGMAGEYGSSVTYVIESWPVHLRNKASGFLISGFSIGGGL
                     AAQVYSIVVPLWGWRSLFFVGMLPILFAFYLRKNLPESDDWQKRQQENKPVRTMVDIL
                     YREKNKYINILLSCIAFACLYVCFSGVTANAALITVMALCCAAVFISFIYQGMGKRWP
                     TGIMLMLVVMFCFLYGWPLQAFLPTWLKVDMQYSPETVALIFMLAGFGSAAGSCIGGF
                     MGDWLGTRKAYVISLLIGQLVIIPVFLVDRDYVWLLGLLIFTQQVFGQGIGALVPKII
                     SGYFNVEQRAAGLGFIYNVGSLGGACAPILGAVVASHTSLGTAMCSLAFILTFVVLVL
                     IGFDMPSRVQRWIHPEAALEYDTVDGKPFYGARKKNVAEE"
     gene            1575..2405
                     /locus_tag="NBI74_004534"
     CDS             1575..2405
                     /locus_tag="NBI74_004534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000948499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCD1934744.1"
                     /translation="MLSGNKKMIHYRENIYSLDNYERYITDSSLFENTGDGIIYTGIV
                     PLITVVEPVVSNQHSVLIIPGGGYRRIVYEREGLDVGRWIAGQGYTAFILSYRMPSVN
                     GYYSFADAKNALNFISYWAGMSERTFKSVGCMGFSAGGHLAGYITHLHYEEETSVRPD
                     WMALIYPVISLDRSIAHQGCRKRLLDAGESEYSASLENSVTEYMPPVFIVHATDDKDV
                     PVIHSLRYFNSIRDKGHHAEAHFFDKGGHGFGVSGKNGYPIEKWQSLLLEWLAEHKFH
                     "
     gene            2451..3161
                     /locus_tag="NBI74_004535"
     CDS             2451..3161
                     /locus_tag="NBI74_004535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002431274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminic acid channel protein"
                     /protein_id="HCD1934745.1"
                     /translation="MKKYYPLLTVILLGLPLCSQALTLNLRGGYRSASHAYETLIQVS
                     QGWNNGWWASFESNTWNTIHNNENELFALNYNKIDINKTYKIDDKWALIPGIQTQWMS
                     AGSQYNPYLKISYQATPDLNLALRYRYDWKAFRQTDLDGNRARNDQHQFDGYVTYKIN
                     DSWLFAWQTTVYTKVNNFKYGNHKKTATENAFVLQYKMSPVFTPYIEYDYLDKQGVYK
                     GKDNKHENSYRVGVLINF"
     gene            3174..>3469
                     /locus_tag="NBI74_004536"
     CDS             3174..>3469
                     /locus_tag="NBI74_004536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminic acid mutarotase"
                     /protein_id="HCD1934746.1"
                     /translation="MKVKNFIYLPFCLFIGTSVAGALPEIPEPFKYGVGGIENGKIYI
                     GLGSLGNNWYMIDTNQSEKKWTKIAQWPTVPREQATATIIDGKIYVFGGIGKDK"
BASE COUNT          972 a          559 c          823 g         1115 t
ORIGIN      
        1 actctggtct tattggtgca actctttggg accgggattg tattacttaa agtaatactc
       61 cagtgtatta taaatccgct attattgtga ggtttaatat ggactcctgt attcagaaaa
      121 agcaggtttg gtataagcat ctttcaccca gacagtggaa gatattcggt gcggcatgga
      181 caggttattt acttgatggt tttgatttcg ttctgatatc tctggttctt acagaggttc
      241 agcatgaatt cggcttgacg acaatagaag cggcaagcct tatttccgct gcttttattt
      301 ctcgatggtt tggggggctt gctattggcg ctttatcgga caagatgggg cgcaggatgg
      361 caatggtttt aagtatagtg ctattctctc tcggaaccct tgcctgtggt ctggctcctg
      421 gatatgcagt gatgtttatt gctcgcattg ttattgggct tggtatggca ggtgaatatg
      481 gctcaagtgt aacatatgtt atcgaaagct ggcctgttca tctcagaaac aaggcaagtg
      541 gttttttaat ttctggattt tctattgggg gaggtttagc tgcacaggtt tatagcattg
      601 tagtaccact atggggctgg cgttctcttt tctttgtcgg catgcttcca atattatttg
      661 ctttttatct tcgcaagaat cttccggagt ctgatgactg gcaaaaaagg caacaagaga
      721 ataaacccgt cagaacaatg gtggatattc tgtacagaga aaagaataaa tacataaata
      781 ttcttctgtc ttgtattgca tttgcatgtc tatatgtttg tttttcaggc gtcactgcaa
      841 acgctgcttt aatcacagtt atggcattgt gctgtgcggc tgtttttatc agttttatat
      901 atcagggtat ggggaaaaga tggccaacag gcattatgtt gatgcttgtg gttatgttct
      961 gtttcctcta cggatggcca ctgcaggctt ttcttcctac atggcttaag gttgatatgc
     1021 agtattcacc agaaactgtt gctctcatat ttatgctggc aggttttggc tctgcagctg
     1081 gaagttgtat aggtggtttt atgggggact ggcttggaac ccgaaaagca tatgttatat
     1141 cacttcttat cggtcagctt gttattatcc ccgtttttct tgtggaccgg gactatgtat
     1201 ggttacttgg ccttctaatc tttactcagc aggtttttgg tcaggggatt ggtgctctgg
     1261 tgccgaaaat aatcagtggt tattttaatg tggagcagcg tgcggcagga ctggggttta
     1321 tttataatgt aggttcactt ggtggagcat gtgcgcctat tcttggtgca gttgttgcgt
     1381 ctcatacaag tcttggtaca gcaatgtgtt ctcttgcttt tatcttaaca tttgttgttc
     1441 ttgtccttat tggttttgat atgccatcac gtgtacagag atggatccat ccagaagctg
     1501 cgcttgaata tgatactgtc gatggaaaac ctttttatgg agcaagaaaa aagaatgttg
     1561 cagaagaata acggatgcta tcagggaata aaaaaatgat tcattacagg gagaatatat
     1621 attctcttga taattatgag cgttacataa ctgacagttc gctatttgag aatactggcg
     1681 atggcataat atatacaggt attgtgcccc ttattacagt tgttgagcct gttgtatcta
     1741 atcaacatag tgtgctgatt atacctggag gtggatacag gaggattgtt tatgaaagag
     1801 agggacttga tgttggcaga tggatagcag gtcagggata tacggcattt attctttctt
     1861 acagaatgcc ctccgttaat ggttattatt cctttgcaga tgcgaagaat gccctgaact
     1921 ttatttctta ctgggccgga atgtcggaga gaacatttaa gagtgttgga tgtatggggt
     1981 tttcggcagg tggtcatctt gctggatata ttactcatct ccattatgag gaagagactt
     2041 ctgtgcgtcc tgactggatg gctctgatat atccagttat atctcttgac aggagtattg
     2101 ctcatcaggg atgccggaaa cgtcttctgg atgcaggtga aagtgaatat tcagccagtc
     2161 ttgaaaatag tgttacagag tatatgccac cagtctttat tgtgcatgca acagatgata
     2221 aggatgttcc agttattcat tctctgagat attttaactc tattcgggat aagggacatc
     2281 atgcagaagc tcattttttt gataagggag gtcatggatt tggtgtatcc ggaaaaaatg
     2341 gatatccgat agaaaaatgg caaagcctgc ttctggaatg gcttgcggaa cataagtttc
     2401 attgagaaat caaattacaa gtgataaata tatttaatga tgaggcattt atgaaaaaat
     2461 attatccact attaactgta attcttcttg gtttaccttt gtgctctcag gcattaactc
     2521 ttaatctacg tgggggatac agaagcgcca gtcacgctta tgaaacgttg attcaggtta
     2581 gtcaggggtg gaataatggt tggtgggcaa gttttgagtc aaacacctgg aatacaattc
     2641 acaacaatga aaatgaattg tttgcactca attataataa aatagatata aataaaacat
     2701 ataaaataga tgacaaatgg gctctaatcc cagggattca gacacaatgg atgtcagcag
     2761 gaagtcagta taacccttat ctgaaaattt cgtatcaggc aacgccagat cttaatctgg
     2821 ccttacgcta tcgctatgac tggaaagctt tccgacagac ggatttagat ggcaacaggg
     2881 cgcgtaatga tcagcatcag tttgatggtt atgtgacata caaaattaat gattcatggt
     2941 tgtttgcatg gcagaccacg gtatatacga aggttaataa tttcaaatat gggaatcata
     3001 aaaaaacagc aactgaaaat gcatttgttc tgcaatataa aatgtcaccg gtgttcaccc
     3061 catatataga atatgattac ttggataaac aaggggtgta taaggggaaa gataataaac
     3121 atgaaaatag ttaccgtgtc ggtgttctga ttaattttta aaaggtgatt acaatgaaag
     3181 tgaaaaactt tatatatctt cccttctgct tgtttattgg aacgtcggta gccggagctt
     3241 taccggaaat tcctgaacca tttaaatatg gtgtaggagg gatcgagaat ggtaaaatat
     3301 atataggact aggaagtctg ggaaataact ggtatatgat agatacaaac cagagtgaaa
     3361 aaaaatggac taagatagcg caatggccga cagttcccag agagcaggca acagctacta
     3421 ttattgatgg taagatctat gtatttggag gaattggaaa agataaaag
//
LOCUS       DAHHTG010000248         3424 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.254, whole genome shotgun sequence.
ACCESSION   DAHHTG010000248 DAHHTG010000000
VERSION     DAHHTG010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3424
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.254"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            158..436
                     /locus_tag="NBI74_004537"
     CDS             158..436
                     /locus_tag="NBI74_004537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1934747.1"
                     /translation="MAHDTKLHNSDDSAVFASRCGRRFHAFKSDWFQHPPCTEEQAEW
                     IIQCYRRRGYEVKKALSLDYRHWIISVRLPYSERPPRASRTFQQRIWR"
     gene            438..1487
                     /locus_tag="NBI74_004538"
     CDS             438..1487
                     /locus_tag="NBI74_004538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416078.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HCD1934748.1"
                     /translation="MRVLLRPVLVPELGLVVLKPGRESIQIFHNPRVLVEPEPKSMRN
                     LPSGVVPAVRQSLAEDKTLLPFFSNERVIRAAGGVGALSDWLLRHVTSCQWPNGDYHH
                     SETVIHRYGTGAMVLCWHCDNQLRDQTSESLELLAQQNLTAWVIDVIRHAISGTQERE
                     LSLAELSWWAVCNQVVDALPEAVSRRSLGLPAEKICSVYRESDIVPGEQTATSILKQR
                     TKNLAPLPYAHQQQKTPQEKTVVSITVDPESPESFMKLPKRRRWVKEKYTRWVKTQPC
                     ACCGMPADDPHHLIGHGQGGMGTKAHDLFVLPLCRKHHNELHTDTVAFEEKYGSQLEL
                     IFRFIDRALAIGVLA"
     gene            1501..2253
                     /locus_tag="NBI74_004539"
     CDS             1501..2253
                     /locus_tag="NBI74_004539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HCD1934749.1"
                     /translation="MNLEALPKYYSPKSPKLSDDAPATGTGCLTITDVMAAQGMVQSK
                     APLGLALFLAKVGVQDAQFAIEGLLNYAMALDNPTLNKLSEEIRLQIIPYLVNFAFAD
                     YSRSAASKARCEHCSGTGFYNVLREVVKHYRRGESVIKEEWVKELCQHCHGKGEVSTA
                     CRGCKGKGIVLDEKRTRFHGVPVYKICGRCNGNRFSRLPTTLARRHVQKLVPDLTDYQ
                     WYKGYADVIGKLVTKCWQEEAYAEAQLRKVTR"
     gene            complement(2428..2630)
                     /gene="cspF"
                     /locus_tag="NBI74_004540"
                     /pseudo
     CDS             complement(2428..2630)
                     /gene="cspF"
                     /locus_tag="NBI74_004540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416076.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspF"
     gene            2929..3144
                     /gene="cspB"
                     /locus_tag="NBI74_004541"
     CDS             2929..3144
                     /gene="cspB"
                     /locus_tag="NBI74_004541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspB"
                     /protein_id="HCD1934750.1"
                     /translation="MSNKMTGLVKWFNADKGFGFISPVDGSKDVFVHFSAIQNDNYRT
                     LFEGQKVTFSIESGAKGPAAANVIITD"
BASE COUNT          948 a          717 c          859 g          900 t
ORIGIN      
        1 tctaatcgct tttaataata agcgttgtgt ttatcccaac aatctgttgt ttgactttta
       61 ttccattaat gtaggggctt tacactggaa ccagtttatt tatactttat acgccagcct
      121 gaacaactgg cacctgctgc gccagcagag acaaccgatg gcgcacgata ccaaattaca
      181 caattctgat gattctgccg tctttgccag caggtgcgga cggcgttttc atgcattcaa
      241 atcggactgg ttccagcatc caccatgcac tgaagagcag gctgaatgga taattcagtg
      301 ttaccgcagg cgcggatacg aggttaagaa agcccttagc ctcgactacc gtcactggat
      361 aatctcagtc agattgcctt actccgaacg cccaccacgt gcgtcccgca ctttccagca
      421 acggatctgg aggtaacgtg cgggtattac ttagacctgt tctggtgcct gagcttgggc
      481 tggtggtcct taagccgggc cgtgaatcca tacagatatt tcataatcct cgagtgctgg
      541 tggaaccgga accaaaaagc atgcgtaatc tgccatccgg agtcgttcct gccgttcgcc
      601 agtcgctggc ggaagacaaa acattgctgc cgttttttag taacgaacgg gtgattcgtg
      661 ctgctggcgg tgttggcgca ttgtctgact ggctattacg tcatgttaca tcctgccagt
      721 ggcctaatgg cgattaccat cactctgaaa cagtcattca ccgttatggt accggcgcaa
      781 tggtgttgtg ttggcactgc gacaaccaac tgcgtgacca gacatcggaa tcactggagc
      841 tgcttgctca acaaaatctg acagcatggg tgattgacgt catccgtcac gcaataagcg
      901 gtacgcagga gcgggaatta tctttggctg aattatcctg gtgggcggtc tgcaatcagg
      961 tggtggatgc actacctgag gctgtatcgc gtcgttcgct gggattacca gcggaaaaaa
     1021 tctgctcggt gtaccgcgaa agcgacatcg taccgggaga gcagaccgcc accagcatat
     1081 tgaaacaacg cacaaaaaat cttgcaccgt tgccttacgc ccaccagcaa caaaaaacac
     1141 cacaggaaaa gacggtggta agcatcaccg ttgatccaga gtctccggaa tctttcatga
     1201 agctgcctaa acgtcgccgc tgggttaagg agaaatacac acgttgggtt aagacacagc
     1261 cgtgtgcttg ctgcggtatg ccagccgacg atccgcatca tctgattggt cacgggcagg
     1321 gcggaatggg aacaaaagca catgatctct ttgtgttgcc tttgtgcaga aagcatcaca
     1381 acgagctgca tacggataca gtggcatttg aagagaagta tggctcccaa ctggagctga
     1441 tatttcgttt tatcgatcgc gcgctggcaa ttggcgtact ggcgtaagtg gagaacgagc
     1501 atgaaccttg aagccttacc aaaatattac tccccaaaat ctccaaaatt gagcgatgac
     1561 gctccagcga caggcaccgg ttgtttaaca attacggatg taatggcagc gcaggggatg
     1621 gtgcagtcga aagcaccact tgggttggcc ttatttctgg caaaagttgg tgttcaggac
     1681 gctcagtttg cgattgaagg cctgctaaat tacgcgatgg cactggataa cccgacattg
     1741 aacaaattga gtgaagaaat ccggttacag attattcctt acctcgtgaa ttttgccttt
     1801 gctgattact ccaggtctgc ggcaagtaag gctcgctgtg agcattgttc aggtacggga
     1861 ttttataatg tattgcgcga agtggtgaaa cactacagac gcggggaatc tgtaatcaag
     1921 gaagaatggg tgaaggaact atgtcagcat tgccatggta agggcgaagt cagcacagcg
     1981 tgcagagggt gtaagggtaa agggattgtt ctggatgaaa aaagaacccg gtttcatggc
     2041 gtaccggtat ataagatttg tgggcgttgt aatggaaacc ggtttagtcg tttaccgacc
     2101 acgctggcac gacgtcatgt ccagaagctg gtaccagacc tgaccgatta tcagtggtat
     2161 aaggggtatg cggacgtcat tggtaaactg gtaacaaagt gctggcagga agaagcatac
     2221 gcggaagcgc aattgaggaa ggtgacgaga taaatgattt ttgctgaaga tggcgacatg
     2281 atgtttgcat ttttcaaaaa acatggataa gattttctca acgatgggct ttgtgtatcc
     2341 gacgtttaga aaaaagtaga aaacccgctt ataagcgggt ttttgtgctt taaatggggc
     2401 aatagagata ttgaatctca tcccgggata aacattggca gttgaaggtc cacgcgaacc
     2461 atttatccag caaaattcca cgcgtaatcc tgtggtaatt tcttctgcat ctcgaagatt
     2521 gagagctgaa acgtgaagct gggcatcgat acgccatcgg atgggaatat aagacctttg
     2581 ctgcttttgt agtcaaagtt tttgacaatt cctgtcattt taggggacag aaaaactcct
     2641 taatactgat aacctggtgc accatacaca cgttcctgga gaaaactact tttttgatag
     2701 gattgaaggt ggctggatgt ctaaaataaa cattgcttca tatgttcaac tatgagttaa
     2761 tgactgcgtc ggtttgaaga acagacgata tacgaagtag tttactaaag cagttctcat
     2821 ttcaggtgtt attcacttat tccttctttg agtctctcca attaagtacg aagtcgtttc
     2881 tgttatgcaa accatttatg ccgaaaggct caagttaagg aatgtagaat gtcaaataaa
     2941 atgactggtt tagtaaaatg gtttaacgct gataaaggtt tcggctttat ttctcctgtt
     3001 gatggtagta aagatgtgtt tgtgcatttt tctgcgattc agaatgataa ttatcgaacc
     3061 ttatttgaag gtcaaaaggt taccttctct atagagagtg gtgctaaagg tcctgcagca
     3121 gcaaatgtca tcattactga ttaaaattca tcgctcgtct gtatacgata acgaagaagg
     3181 ctgatgcctg agtagagata cggacagagt agtgaatatt ggatctcttt aataaaaagt
     3241 aaggaggtcc aatacatgaa acaatggcta gcatatttgg caaaatctta atcaggaaaa
     3301 gtatgctaac cattgtggtg aagtgcaggt ttgctgcatg aatagtttca cagcagaagc
     3361 taactgctgg catcgcaaaa caaagtgcgt aagtggatga ctcccacaaa aagcaccaca
     3421 atct
//
LOCUS       DAHHTG010000249         3349 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.255, whole genome shotgun sequence.
ACCESSION   DAHHTG010000249 DAHHTG010000000
VERSION     DAHHTG010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3349)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3349)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3349
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.255"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            121..1086
                     /gene="dusB"
                     /locus_tag="NBI74_004542"
     CDS             121..1086
                     /gene="dusB"
                     /locus_tag="NBI74_004542"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HCD1934751.1"
                     /translation="MRIGQYQLRNRLIAAPMAGITDRPFRTLCYEMGAGLTVSEMMSS
                     NPQVWESDKSRLRMVHIDEPGIRTVQIAGSDPKEMADAARINVESGAQIIDINMGCPA
                     KKVNRKLAGSALLQYPDVVKSILTEVVNAVDVPVTLKIRTGWAPEHRNCEEIAQLAED
                     CGIQALTIHGRTRACLFNGEAEYDSIRAVKQKVSIPVIANGDITDPLKARAVLDYTGA
                     DALMIGRAAQGRPWIFREIQHYLDTGELLPPLPLAEVKRLLCAHVRELHDFYGPAKGY
                     RIARKHVSWYLQEHAPNDQFRRTFNAIEDASEQLEALEAYFENFA"
     gene            1112..1408
                     /gene="fis"
                     /locus_tag="NBI74_004543"
     CDS             1112..1408
                     /gene="fis"
                     /locus_tag="NBI74_004543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001465625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator Fis"
                     /protein_id="HCD1934752.1"
                     /translation="MFEQRVNSDVLTVSTVNSQDQVTQKPLRDSVKQALKNYFAQLNG
                     QDVNDLYELVLAEVEQPLLDMVMQYTRGNQTRAALMMGINRGTLRKKLKKYGMN"
     gene            1493..2377
                     /gene="yhdJ"
                     /locus_tag="NBI74_004544"
     CDS             1493..2377
                     /gene="yhdJ"
                     /locus_tag="NBI74_004544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417728.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HCD1934753.1"
                     /translation="MRTGCEPTRFGNEAKTIIQGDALTELKKLPAESVDLIFADPPYN
                     IGKNFDGLIEAWKEDLFIDWLFEVIAECHRVLKKQGSMYIMNSTENMPFIDLQCRKLF
                     TIKSRIVWSYDSSGVQAKKHYGSMYEPILMMVKDAKNYTFNGDAILVEAKTGSQRALI
                     NYRKNPPQPYNHQKVPGNVWDFPRVRYLMDEYENHPTQKPEALLKRIILASSNPGDIV
                     LDPFAGSFTTGAVAVASGRKFIGIEINSEYIKMGLRRLDVASHYSAEELAKVKKRKTG
                     NLSKRSRLSEVDPDLIAK"
     gene            2461..2640
                     /locus_tag="NBI74_004545"
     CDS             2461..2640
                     /locus_tag="NBI74_004545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2556 family protein"
                     /protein_id="HCD1934754.1"
                     /translation="MIRKYWWLVVFAVFVFLFDTLLMQWIELLATETDKCRNMNSVNP
                     LKLVNCDELNFQDRM"
     gene            complement(2643..3305)
                     /gene="acrS"
                     /locus_tag="NBI74_004546"
     CDS             complement(2643..3305)
                     /gene="acrS"
                     /locus_tag="NBI74_004546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrS"
                     /protein_id="HCD1934755.1"
                     /translation="MAKRTKAEALKTRQELIETAIAQFAQHGVSKTTLNDIADAANVT
                     RGAIYWHFENKTQLFNEMWLQQPSLRELIQDHLTAGLEHDPFQQLREKLIVGLQYIAK
                     IPRQQALLKILYHKCEFNDEMLAEGVIREKMGFNPQTLREVLQACQQQGCVANNLDLD
                     VVMIIIDGAFSGIVQNWLMNMAGYDLYKQAPALVDNVLRMFMPDENITKLIHKTNELS
                     VI"
BASE COUNT          918 a          775 c          795 g          861 t
ORIGIN      
        1 attttaaatg caattctttg atccatctca gaggattggt caaagtttgg cctttcatct
       61 cgtgcaaaaa atgcgtaata tacgccgcct tgcagtcaca gtatggtcat ttcttaactc
      121 atgcgcatcg gacaatatca gctcagaaat cgcctgatcg cagcgcccat ggctggcatt
      181 acagacagac cttttcggac gttgtgctac gagatgggag ccggattgac agtatccgag
      241 atgatgtctt ctaacccaca ggtttgggaa agcgacaaat ctcgtttacg gatggtgcac
      301 attgatgaac ccggtattcg caccgtgcaa attgctggta gcgatccgaa agaaatggca
      361 gatgcagcac gtattaacgt ggaaagcggt gcccagatta ttgatatcaa tatgggttgc
      421 ccggctaaaa aagtgaatcg caagctcgca ggttcagccc tcttgcagta cccggatgtc
      481 gttaaatcga tccttaccga ggtcgtcaat gcagtggacg ttcctgttac cctgaaaatt
      541 cgcactggct gggcaccgga acaccgtaac tgcgaagaga ttgcccaact ggctgaagac
      601 tgtggcattc aggctctgac cattcatggc cgtacacgcg cctgtttgtt caatggagaa
      661 gctgagtacg acagtattcg ggcagttaag cagaaagttt ccattccggt tatcgcgaat
      721 ggtgacatta ctgacccgct taaagccaga gctgtgctcg actatacagg ggcggatgcc
      781 ctgatgatag gccgcgcagc tcagggaaga ccctggatct ttcgggaaat ccagcattat
      841 ctggacactg gggagttgct gcccccgctg cctttggcag aggttaagcg cttgctttgc
      901 gcgcacgttc gggaactgca tgacttttat ggtccggcaa aagggtaccg aattgcacgt
      961 aaacacgttt cctggtatct ccaggaacac gctccaaatg accagtttcg gcgcacattc
     1021 aacgccattg aggatgccag cgaacagctg gaggcgttgg aggcatactt cgaaaatttt
     1081 gcgtaaacag aaataaagag ctgacagaac tatgttcgaa caacgcgtaa attctgacgt
     1141 actgaccgtt tctaccgtta actctcagga tcaggtaacc caaaaacccc tgcgtgactc
     1201 ggttaaacag gcactgaaga actattttgc tcaactgaat ggtcaggatg tgaatgacct
     1261 ctatgagctg gtactggctg aagtagaaca gcccctgttg gacatggtga tgcaatacac
     1321 ccgtggtaac cagacccgtg ctgctctgat gatgggcatc aaccgtggta cgctgcgtaa
     1381 aaaattgaaa aaatacggca tgaactaatt caggttagct aattgcttga ttaaaaggcg
     1441 ctactcggca tggggaagcg ccttttttat aggtgtcaca aagggagtga ccatgagaac
     1501 aggatgtgaa ccgacccgat ttggtaatga agcaaagacc atcatccagg gtgatgcatt
     1561 aaccgaactt aaaaagctcc ccgccgaaag tgtcgatctg atctttgccg acccaccgta
     1621 taacatcggt aaaaattttg atggtctgat cgaagcctgg aaagaagatc tgtttatcga
     1681 ctggctgttt gaagtgattg cagagtgcca tcgcgttctg aaaaagcagg gcagcatgta
     1741 catcatgaat agcacggaaa acatgccctt tatcgatctc cagtgccgca agctttttac
     1801 catcaaaagt cgcatcgtct ggtcatatga cagttctgga gtgcaggcga aaaaacacta
     1861 cggctccatg tacgaaccca tcctgatgat ggtgaaagac gcaaagaact acacgttcaa
     1921 cggtgatgct attctggtag aagccaaaac cggatcgcag cgcgcgttga tcaattatcg
     1981 caaaaatcct ccacagcctt acaatcatca aaaagtaccg ggtaacgtct gggattttcc
     2041 gcgcgtgcgt tatttgatgg atgaatatga aaaccacccg acgcaaaaac cggaagcctt
     2101 gctgaaacgc attattctcg cctcttccaa cccaggcgat atcgttctcg acccgtttgc
     2161 cggtagcttt actaccggtg ccgtagccgt cgccagcggg cgaaaattca ttggtattga
     2221 gatcaacagc gagtacatca aaatggggct tcgacggctg gatgtcgcgt cgcattactc
     2281 tgcggaagaa ctggcgaaag tgaaaaaaag aaagaccggc aacctgtcaa aacgaagccg
     2341 gttaagcgaa gttgaccccg atctcattgc aaagtaaagg aagtgtaagc ctggttttca
     2401 gattattcat ttcgtgtata tttcctgcca gacttggtta aacatgcaca ggctcaggta
     2461 atgattcgca agtattggtg gctcgtcgtt ttcgctgtct tcgtttttct gtttgatact
     2521 ttactgatgc agtggattga actgctggca acagaaacag acaaatgccg caatatgaac
     2581 tcagttaatc cactaaaact ggttaactgt gacgaactga attttcagga cagaatgtga
     2641 atttatatga cacttaattc attcgtttta tgaattaatt tcgttatgtt ttcatctggc
     2701 atgaacattc ttaatacgtt atcgaccaga gcgggggctt gtttataaag atcataaccc
     2761 gccatattca ttaaccagtt ttgaacaatt ccgctgaagg caccatcaat aataatcatc
     2821 acaacatcta aatcgagatt atttgctaca caaccttgtt gctgacacgc ctgcaatact
     2881 tcgcggagag tctgcggatt aaagcccatc ttttcgcgta tcactccctc ggccagcatc
     2941 tcatcattaa attcacattt gtgatataag attttcagca acgcctgctg gcggggaatt
     3001 ttggcaatat attgcaaacc gacaatcaat ttttcacgca attgctgaaa cgggtcatgc
     3061 tctaacccag cggtcaagtg atcctggatt aactcccgca atgaaggctg ttgcaaccac
     3121 atctcattaa acagttgagt cttgttttcg aagtgccagt agatagcgcc acgcgtaacg
     3181 ttagcagcgt cggcaatgtc gttgagcgtc gtcttgctta cgccatgctg cgcaaactgg
     3241 gcgatggcag tttcaatcag ttcttgccgg gtcttcaggg cttcagcttt ggttcttttt
     3301 gccatgatta attattcagg aaataaatat attcgacaca ggatgagaa
//
LOCUS       DAHHTG010000250         3320 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.256, whole genome shotgun sequence.
ACCESSION   DAHHTG010000250 DAHHTG010000000
VERSION     DAHHTG010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3320)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3320)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3320
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.256"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            <1..978
                     /locus_tag="NBI74_004547"
     CDS             <1..978
                     /locus_tag="NBI74_004547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001574080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2345 domain-containing protein"
                     /protein_id="HCD1934756.1"
                     /translation="FEEGNPDRPYIAGVKHDSAHTDHVTIQNYKRNVLRTPANNKIRL
                     DDERGKEHIKVSTEYGGKSQLNLGHLVDAGKQQRGEGFELRTDLWGAVRAKKGIFISA
                     DAQDKAQGQVREMADIISELNSLSDKIQKLSDDAATANADPADMAAQVALITSRINDL
                     TASVILMHAPKGVAVASGEHLQLAAVKNLQINAGNNADIGVVKNMFIGVGRALSVFVR
                     KAGIKLIANKGAVSVQAQHDLMELLAKKSIEIVSTEDEIRISAKKKITINGGGSYIRI
                     EGSGIEPGTPGDYNVKAVHYGRMGKAHEPVELQMLAEKVDEPPVKFFFS"
     gene            990..>3320
                     /locus_tag="NBI74_004548"
     CDS             990..>3320
                     /locus_tag="NBI74_004548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family peptidase"
                     /protein_id="HCD1934757.1"
                     /translation="MIISPPFIRAKNNNENDAHWIERMMPVEPDRDYPINYGGSWHGG
                     IHVRHTNSDRQPEYVRAIADGVVVSIRNPSDQAACSLPPLNYNGSTDDGYVLLRHETE
                     IGTGEDGNIVYYSLYMHLEKIEEGISEGNKVYRKSSLGMPGHVDGDRGLHFQIFCDDD
                     NLRKITGRTTPEMDVSRHGRTDVVYGDIYFYLPPGTRFYRSAPENNAVDNTGEVFQST
                     EPLVVTMCFDTGHCIMTTRRANPVIPSRYDIVGEVLTDADGTDYEYNLYSAALRLYPQ
                     SPSAGFELLRFGRVINTEHETLIPANAPLWRTVSYPGGQGVINLAADVVTKFSDGDFP
                     HWSGWRLVDDDADSNSQCNSVILRGGGFTDFRQMICHFPLEWCNTGIEARYGWLKEGV
                     IQERQENEANSDLSLGTDYYRPNNVEYNTTGDSHGESPQSAISEMPDLSYEQSNAYEN
                     AVRVLLLGEEDWQTLVKHIKALCFDTSSIAEGRVWHFNPGEFIKHFRKCGWVDKAILK
                     RIFASGTNDRDRAVIEQKVDIYGTAINIVMNKYIMNSPLRRAHFLGQGAVESSRLRSM
                     QEKSQYQTVDENGRPVGGGIVPDSLRDENSDLGHWYGAIETEVDGYFSGVKYNSGGGR
                     IAGSYDWILGNCDTEDAQKFRGRGFKQLTGRSNYAEYWVYRAWIDTNSFTAKWWEDPL
                     WRLHDRRRLTRIPANIEEPHRVTRSEYNCIDTGGFFIVKTVDRRGTRSSITRAMDQDS
                     EVIHLNNTTIDTTDEQTIRSVSRAINGGENGLPDRIT"
BASE COUNT          937 a          663 c          929 g          791 t
ORIGIN      
        1 tttgaggaag gcaacccgga ccgaccttat atcgccgggg taaaacacga ctcggcacac
       61 accgaccatg tgaccattca gaactacaag cgtaacgtgc tgcgcacccc ggcgaacaac
      121 aaaatccgcc tcgacgatga gcgcggcaaa gagcatatca aggtcagtac cgagtacggc
      181 ggcaagagcc agctaaacct cggccatctg gtggatgccg gaaaacagca gcgcggggaa
      241 ggttttgaac tgcgtaccga tctgtggggt gcagtacggg caaagaaagg catctttatc
      301 agcgccgacg cgcaggataa ggcgcagggt caggtacgcg aaatggccga cattatcagc
      361 gaactgaaca gcctgagcga taaaatccag aaactgtctg acgatgcggc cacggcgaac
      421 gccgatccgg cggatatggc cgcgcaggtg gcgctgataa ccagccggat aaatgacctg
      481 accgcctcgg tgattttgat gcacgcgccg aaaggtgtgg cggtcgccag cggcgagcac
      541 ctgcaactgg ccgcagtgaa aaacctccag ataaatgcgg gaaacaacgc cgatatcggc
      601 gtggtgaaga atatgtttat cggcgtaggg cgggcgctga gcgtgtttgt gcgcaaggcg
      661 ggtatcaaac tgattgccaa taaaggcgca gtatcggtac aggcgcagca cgatctgatg
      721 gagttactgg cgaagaagtc gatagagatc gtcagtactg aggacgagat caggatttca
      781 gcgaagaaaa aaataacgat taatggtggt ggaagttata tccggattga gggtagcggc
      841 attgagccgg gaacgccggg ggattataac gtcaaagcgg tgcattacgg gcgtatggga
      901 aaagcacatg agcctgttga gttacaaatg ttggcagaga aagtagatga gccacccgta
      961 aaatttttct tttcttaagg atataagaaa tgattattag cccgccattt atacgagcga
     1021 aaaataacaa tgaaaacgat gctcattgga ttgaaaggat gatgccagtt gaacctgacc
     1081 gtgactatcc catcaactat gggggatcat ggcatggagg catccacgtt agacatacaa
     1141 acagtgacag gcaaccagaa tatgtccgtg ccattgctga tggtgtcgtg gtatctattc
     1201 gcaatccctc tgaccaggcg gcatgtagtc ttccgccgct caattataat ggaagcacag
     1261 acgatgggta tgttctgctt cgtcatgaaa ctgaaatagg cactggtgag gacggcaata
     1321 ttgtttatta ctctctttac atgcacctgg aaaagattga agagggtatc agtgaaggaa
     1381 ataaagtcta ccggaaatcg tcactgggaa tgccggggca tgtcgacggg gatcgcggcc
     1441 tgcacttcca aattttctgc gatgatgaca atctgagaaa aataaccggg cgcactacac
     1501 ctgagatgga tgtgagccgt catgggcgta ccgacgtggt ttacggggat atctattttt
     1561 acctgccgcc cggtacccgc ttttacagga gtgcgccgga aaacaacgca gtagataaca
     1621 caggtgaagt ttttcagagc acggaaccgt tggttgtcac catgtgtttt gatactggcc
     1681 attgtatcat gactacccgc agagcaaatc cggtgatacc aagccgttat gacattgtgg
     1741 gagaagtgtt gacggatgct gacggcactg attatgaata caacttgtac agtgctgcac
     1801 tgagacttta tccgcaaagt ccaagcgcag ggtttgagtt actgcgcttt ggtcgggtta
     1861 tcaataccga gcatgaaaca ctgatacccg cgaatgcgcc actgtggcga accgtgagct
     1921 atcccggtgg gcaaggagtg ataaatctgg cggcagatgt tgtgacaaaa tttagcgacg
     1981 gtgattttcc gcactggagt ggctggcgac tggtagatga cgacgctgac agcaacagtc
     2041 agtgcaattc agtaatatta cgtggtggtg gttttacgga ttttcgtcag atgatttgcc
     2101 acttcccact ggagtggtgt aatacgggaa tagaagcgcg ttacggctgg ttgaaagaag
     2161 gggtaataca ggaacgtcag gaaaatgagg ctaacagcga tttatcatta ggtacggatt
     2221 actacaggcc aaataatgtc gaatataata cgactggaga tagccatgga gaatctcctc
     2281 agtcagctat atcagaaatg ccagatttgt cttatgaaca gtcaaatgct tatgaaaatg
     2341 ccgtcagagt tttattactg ggagaagaag actggcagac gttggtgaag catattaagg
     2401 cactgtgttt tgataccagt agtattgctg aaggacgggt ttggcatttt aatccgggtg
     2461 agtttattaa gcattttagg aagtgtgggt gggttgacaa agcaatattg aagcgaattt
     2521 ttgcctctgg tactaatgac cgtgatagag cggttattga acaaaaagtt gatatatatg
     2581 gtacggctat aaatattgtt atgaataaat atataatgaa ttctcctctg agacgtgctc
     2641 actttctggg gcaaggggca gtggaatctt cccgtttaag aagtatgcag gaaaaaagcc
     2701 agtatcaaac tgttgatgaa aatggtcggc cagtcggcgg aggtattgtt ccggactcat
     2761 tacgtgatga gaattcggac ttgggtcatt ggtatggtgc aattgagact gaggtcgatg
     2821 gatacttctc tggtgttaaa tataattctg gtggaggacg aattgcaggc tcatatgact
     2881 ggatactagg aaactgcgac actgaggatg cccagaagtt tcgtgggcga ggttttaagc
     2941 agttaactgg tcgatcaaat tatgccgaat attgggtcta tcgtgcctgg atagatacga
     3001 atagttttac cgctaagtgg tgggaagacc ctctgtggcg cttacatgat cgtcgtcgtt
     3061 taactcgcat accggctaat atcgaagagc ctcacagggt taccagaagc gaatataact
     3121 gtatcgatac tggtggattc tttattgtta agaccgttga tcgtcggggt actcggtcaa
     3181 gtattaccag agcgatggat caagacagtg aagttattca tttgaacaat accacgatag
     3241 atacaacgga tgaacaaaca ataagaagtg tgtcccgtgc aattaatggt ggtgaaaatg
     3301 gtttgccaga caggataacc
//
LOCUS       DAHHTG010000251         3303 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.257, whole genome shotgun sequence.
ACCESSION   DAHHTG010000251 DAHHTG010000000
VERSION     DAHHTG010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3303)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3303)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3303
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.257"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            504..694
                     /locus_tag="NBI74_004549"
                     /pseudo
     CDS             504..694
                     /locus_tag="NBI74_004549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308353.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(764..904)
                     /gene="ykgO"
                     /locus_tag="NBI74_004550"
     CDS             complement(764..904)
                     /gene="ykgO"
                     /locus_tag="NBI74_004550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L36"
                     /protein_id="HCD1934758.1"
                     /translation="MKVLNSLRTAKERHPDCQIVKRKGRLYVICKSNPRFKAVQGRKK
                     KR"
     gene            complement(904..1167)
                     /locus_tag="NBI74_004551"
     CDS             complement(904..1167)
                     /locus_tag="NBI74_004551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HCD1934759.1"
                     /translation="MKPNIHPEYRTVVFHDTSVDEYFKIGSTIKTDREIELDGVTYPY
                     VTIDVSSKSHPFYTGKLRTVASEGNVARFTQRFGRFVSTKKGA"
     gene            complement(1532..1633)
                     /locus_tag="NBI74_004552"
     CDS             complement(1532..1633)
                     /locus_tag="NBI74_004552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1934760.1"
                     /translation="MKENKVQQISHKLINIVVFVAIVEYAYLFLHFY"
     gene            complement(2106..3248)
                     /locus_tag="NBI74_004553"
     CDS             complement(2106..3248)
                     /locus_tag="NBI74_004553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HCD1934761.1"
                     /translation="MTNKHPSLFSPFMLTEKIKLRNRIVMAPMTTWSANPDGTISEQE
                     LEFYKRRSQNVGLVITGCTYVTPSGIGFTHEFAAYDDRFINSLEKLAAAAKSGGAPAI
                     LQIFHAGNKAIPELVPNYDVISASASSVKSGDFMKRVVQSREMTENEIQETIRAFGDV
                     TKRAIKAGFDGVELHGAHGFLLQNFFSPLFNQRNDRWGGDLEGRMRFPLAVLQEVKNV
                     VYEYATKPFAIGYRISPEESVTGGLRIEDTYKLLDRLISSGISYIHTSLVSINDSYPV
                     ESPNGPRTIELILNHIAGRVPVIAAGKIRTPSQAQEAISAGLPLVAIGKGLVINPEWV
                     TLAESGRSHEIQTALNPQRVPELTIPDKLWDQIQASKGTGWFPLMD"
BASE COUNT          940 a          671 c          709 g          983 t
ORIGIN      
        1 acgttcaatt cttacttgca ttttatatta gccaggaact ttgccctgag gtgttaggaa
       61 tagtcttaat gtgttgtgta atggactagt aaaaaaacaa gtttaaatga taagaaatag
      121 aatataaaac tatcataagt cataattaaa gatgatttgc caatcagtaa ggtgttttaa
      181 tggattaaca tgccgcacaa aatggttatg ggtgttcatt ccaataagtg atggaaaatt
      241 aaacaggtgt aagtacgtca tctgtccggt aagcccccta agagctaaat taagtgaagt
      301 taaacgaaga ctgccagttg tcacaggaac gatggcctct ttgccagggc actgcattca
      361 gtcatctgtg cgcgttactc taactggcac tcgaacgtcg gcgatggggt taccgatgga
      421 tctggccgtt cctgtggcgt aaacgttttt atgtcagtac caacagattt accacattta
      481 tcatcttaac agtttgagtg taaatgcagg ctgcgtcgca aagggaggaa actgagtggc
      541 gtgttcagtc taaaagagag ttgatgccag catacagggc gaagctggtc aacaagtcaa
      601 cattaatata atggaatatt cggagagaaa cgtcaggcag ttgaccttaa atgaacagga
      661 agatacatcc ccaggaaaat taatgctggt gtaataaata caccaacatt aatccggtct
      721 gattattaat cagacaaata tcgttaattc gtcgaatttt aaatcaacgt tttttcttac
      781 gaccctgaac ggccttaaaa cgtggattag atttacaaat cacatacagc cgtccttttc
      841 gcttcacaat ctgacagtct ggatggcgtt cttttgcggt acgcagagag ttaaggactt
      901 tcatcatgcc ccctttttcg tgctaacaaa acgaccaaaa cgttgggtga accgtgcaac
      961 atttccttct gatgccactg ttctcagctt ccctgtatag aacgggtgcg atttcgaaga
     1021 gacatcaatt gttacgtacg gatacgttac gccatccagc tcaatctcac ggtctgtttt
     1081 gatagtcgag ccgattttaa agtactcatc aacgctggtg tcgtggaaca ccacagtacg
     1141 atactcagga tggatattgg gtttcatcat ttttacctgt tatgttataa cataaccgta
     1201 aagatacatg ctcatccgga tcatttcaac acccaatcac tgtgactatt tgtgggatgt
     1261 ggaatttccg caacggggaa gtctggcatt gttagcagcc attggcgacg ctcccataaa
     1321 cgcctggatg agtgattgat gatggtttcg ctgagagtat ggatttttcg agaatggagg
     1381 ctatctccac tttactggcc gatgatttat tgcagcatga ttgattacct gacaatgtgt
     1441 attcctctcc cacggatata ataattactt acaaaaaagg gagaggatgc atattttaaa
     1501 tatcactgaa gtgaacagtt tattgccgtt attaatagaa atggagaaat aaataggcgt
     1561 attctacaat tgcgacaaaa acaacgatat taatcagttt atgactgatt tgctgtactt
     1621 tattctcttt cattggtact tcctcgcttt aaaaaagagt gcacttcgta agtgccctta
     1681 tataaataac gagtttggtc aaccaatttt ttgacatgta tcacaaattt gaatagatgt
     1741 attacatcgg ctatctttta ttgccccaac gtcattgata tatgtcgcct gaaatcagtt
     1801 acgggaatga gtctgatctc aagactggcc cagtcctggc gttgattggc gctgaggagc
     1861 atatcgcatc tcatcgtaac gtcgtatctc ctggggtgtt atacaagata tcgttgtagg
     1921 taaccaggga gaggaattga gtctattaaa ccgtcaacta tgccggatac atactggatt
     1981 acactgcagg cacgccttgc gagagaacgt gccgcagtga cgggttaatt atctgaaaga
     2041 atttgcgagg ctgtatcggt tactcattga tttgatagtt ttactctcgg gagagtaatc
     2101 gatatttaat ccattaacgg aaaccagcca gttcctttcg atgcctgaat ttgatcccat
     2161 agtttatccg gaatggttaa ttcaggaacg cgttgtggat taagggctgt ttgaatttca
     2221 tgacttctac ctgattcagc taaagtcacc cattctggat ttatcaccag ccctttaccg
     2281 atcgcaacaa gtggcaggcc agcggaaatt gcttcttgag cctgagacgg tgtgcgtatt
     2341 ttacctgcgg cgattaccgg cactctgcct gcaatatgat ttaaaatcaa ttcaatagtt
     2401 cggggaccat ttggcgactc aacagggtag ctatcgttaa tactgaccag cgaggtatgt
     2461 atatacgaaa taccggatga aatgagccga tcaaggagtt tataagtatc ctctatccgc
     2521 agtccccctg ttacagattc ttcaggagat attctgtaac cgatagcaaa gggctttgtc
     2581 gcatattcat agactacatt ttttacttcc tgtaatacgg ccagcgggaa gcgcatacgt
     2641 ccttcaagat ctcctcccca tcggtcatta cgctggttaa atagagggga gaagaaattc
     2701 tgcagaagga atccgtgcgc accatgaagt tcaacgccat caaaacctgc tttaatcgcc
     2761 ctttttgtga catctccgaa tgctctaatg gtttcctgaa tttcgttttc agtcatttcc
     2821 ctgctttgta ccactctctt cataaagtca ccagatttta cgcttgatgc gctggcacta
     2881 ataacatcgt aatttggcac aagctccggg atggctttat taccggcgtg gaaaatctgc
     2941 aaaatagccg gagcaccgcc acttttggct gcagctgcta acttttcaag gctgttaatg
     3001 aaacggtcgt catacgcggc aaattcatga gtaaacccaa tgccgcttgg cgtgacatag
     3061 gtacatcctg ttattactaa accaacattt tgtgagcgac gtttataaaa ttcgagttct
     3121 tgctcagaga tggttccatc aggattagct gaccaggttg tcataggagc cataacgata
     3181 cgattacgta gctttatttt ttctgtcagc ataaaaggag aaaacaatga tgggtgttta
     3241 tttgtcataa caatacctca aatgtttatc tgattttaat gcgtataatc ttaataagtt
     3301 gag
//
LOCUS       DAHHTG010000252         3238 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.259, whole genome shotgun sequence.
ACCESSION   DAHHTG010000252 DAHHTG010000000
VERSION     DAHHTG010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3238)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3238)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3238
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.259"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            complement(62..589)
                     /gene="mobB"
                     /locus_tag="NBI74_004554"
     CDS             complement(62..589)
                     /gene="mobB"
                     /locus_tag="NBI74_004554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein B"
                     /protein_id="HCD1934762.1"
                     /translation="MAGKTMIPLLAFAAWSGTGKTTLLKKLIPALCARGIRPGLIKHT
                     HHDMDVDKPGKDSYELRKAGAAQTIVASQQRWALMTETPDEEELDLHFLASRMDTSKL
                     DLILVEGFKHEEIAKIVLFRDGAGHRPEELVIDRHVIAVASDVPLNLDVALLDINDVE
                     GLADFVVEWMQKQDG"
     gene            complement(571..1155)
                     /gene="mobA"
                     /locus_tag="NBI74_004555"
     CDS             complement(571..1155)
                     /gene="mobA"
                     /locus_tag="NBI74_004555"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase MobA"
                     /protein_id="HCD1934763.1"
                     /translation="MNLMTTITGVVLAGGKARRMGGVDKGLLELNGKPLWQHVADALM
                     TQLSHVVVNANRHQEIYQVSGLKVIEDSLADYPGPLAGMLSVMQQEAGEWFLFCPCDT
                     PYIPHDLAARLTHQRKDAPVVWVHDGERDHPTIALVNRAIEPLLLEYLQAGERRVMAF
                     MRLAGGHAVDFSDRKEAFINVNTPEELARWQEKR"
     gene            1225..1494
                     /locus_tag="NBI74_004556"
     CDS             1225..1494
                     /locus_tag="NBI74_004556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihD family protein"
                     /protein_id="HCD1934764.1"
                     /translation="MKCKRLNEVIELLQPAWQKEPDLNLLQFLQKLAKESGFDGELAD
                     LTDDILIYHLKMRDSAKDAVIPGLQKDYEEDFKTALLRARGVIKE"
     gene            1571..2557
                     /gene="srkA"
                     /locus_tag="NBI74_004557"
     CDS             1571..2557
                     /gene="srkA"
                     /locus_tag="NBI74_004557"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response kinase SrkA"
                     /protein_id="HCD1934765.1"
                     /translation="MNNSAFTFQTLHPDTIMDALFEHGIRVDSGLTPLNSYENRVYQF
                     QDEDRRRFVVKFYRPERWTADQILEEHQFALQLVNDEVPVAAPVAFNGQTLLNHQGFY
                     FAVFPSVGGRQFEADNIDQMEAVGRYLGRMHQTGRKQLFIHRPTIGLNEYLIEPRKLF
                     EDATLIPSGLKAAFLKATDELIAAVTAHWREDFTVLRLHGDCHAGNILWRDGPMFVDL
                     DDARNGPAIQDLWMLLNGDKAEQRMQLETIIEAYEEFSEFDTAEIGLIEPLRAMRLVY
                     YLAWLMRRWADPAFPKNFPWLTGEDYWLRQTATFIEQAKVLQEPPLQLTPMY"
     gene            2574..3200
                     /gene="dsbA"
                     /locus_tag="NBI74_004558"
     CDS             2574..3200
                     /gene="dsbA"
                     /locus_tag="NBI74_004558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA"
                     /protein_id="HCD1934766.1"
                     /translation="MKKIWLALAGLVLAFSASAAQYEDGKQYTTLEKPVAGAPQVLEF
                     FSFFCPHCYQFEEVLHISDNVKKKLPEGVKMTKYHVNFMGGDLGKDLTQAWAVAMALG
                     VEDKVTVPLFEGVQKTQTIRSASDIRDVFINAGIKGEEYDAAWNSFVVKSLVAQQEKA
                     AADVQLRGVPAMFVNGKYQLNPQGMDTSNMDVFVQQYADTVKYLSEKK"
BASE COUNT          821 a          779 c          790 g          848 t
ORIGIN      
        1 gcatatgtgc ggaataaatg ccggatgcga cgctggcgcg tttgcttcag cctattcttg
       61 cttatccgtc ctgcttttgc atccactcca ccacaaaatc agccagcccc tcaacatcat
      121 taatatccag taacgcgaca tcaagattaa gcggcacatc actggctaca gcaataacat
      181 gcctgtctat cactaattct tccggtcgat gtccggctcc atcgcgaaac agcacaatct
      241 ttgcgatctc ttcatgctta aacccttcga ccagaatcaa atccagcttt gaggtatcca
      301 ttcgacttgc gagaaaatgt agatccagct cttcttcgtc tggtgtttcc gtcatcaagg
      361 cccatcgctg ctggctggca acgatggttt gcgccgcgcc agccttgcgc agctcatagc
      421 tatctttgcc cggtttatca acatccatat catggtgcgt atgcttaatc agccccggac
      481 ggatccctct ggcgcataat gccgggatca attttttcag cagcgttgtt tttccggtgc
      541 cactccatgc agcaaaggcg agtaacggta tcatcgtttt tcctgccatc gggcaagctc
      601 ctctggcgta ttcacgttta taaatgcctc tttacgatcg ctgaaatcaa ccgcatgacc
      661 gccagccaga cgcataaatg ccataacccg gcgttctcct gcttgcagat attccagtaa
      721 taaaggctca attgcgcggt ttaccagagc aatagtcggg tgatcgcgtt caccgtcgtg
      781 gacccacacg acaggcgcat ctttgcgctg atgagtaagc cgggcggcta aatcatgggg
      841 aatgtaaggc gtatcgcacg ggcaaaacaa aaaccactca cccgcttcct gctgcattac
      901 tgaaagcatt cctgccagag ggccagggta atccgccagt gaatcttcaa tcactttcag
      961 accgcttacc tgatagattt cctgatgacg attagcatta accacgacgt gagaaagctg
     1021 cgtcataagc gcgtcagcga catgttgcca taatggtttg ccgtttaatt caagcaatcc
     1081 tttatctacg ccgcccattc gtctggcttt accgcccgcc agcacaacgc ctgttatcgt
     1141 cgtcatcaga ttcaccgata tcgcctcttt tattgtggga ttgaccctgc taacgtgtct
     1201 gtctcaagag taaggagcat tactatgaaa tgtaaacgtc tgaatgaagt tattgaactc
     1261 ctccagccag cctggcaaaa agagccagac cttaacctgc tgcaattttt gcagaaactg
     1321 gcgaaagagt caggttttga cggcgaactg gcggatttga cggatgacat tctgatctat
     1381 cacctgaaaa tgcgtgattc cgcgaaagat gcggtgatcc cgggtttgca gaaagattat
     1441 gaagaagatt tcaaaacggc gctattacgc gcacgtggcg taattaaaga gtaaaagctt
     1501 gtaagcggcg caaccaaaat catcgtgaaa tgatatcctt catcattcgt aatgttttcc
     1561 ggatgatggg atgaacaaca gcgcttttac tttccagaca ctacacccgg ataccatcat
     1621 ggacgctctg tttgagcatg ggattcgggt ggattccggt cttaccccgc ttaacagcta
     1681 tgaaaaccgt gtctatcaat ttcaggacga agatcgtcga cgttttgtcg tcaaatttta
     1741 tcgccctgaa cgttggacag ccgatcaaat cctcgaagaa catcaatttg cgttgcagct
     1801 ggttaacgat gaagttccgg tcgcagcacc tgtggccttt aatggtcaga ctttattgaa
     1861 tcatcaggga ttttatttcg ctgtttttcc aagcgtcggt ggtcgccagt tcgaagctga
     1921 taatatcgat cagatggaag cggttgggcg ttatttaggg cgtatgcacc agacggggcg
     1981 caaacagctt tttatccatc gcccgaccat cggtttgaat gaatatctca ttgagccacg
     2041 caagctgttt gaggacgcta cactgatacc ttccgggttg aaagcggcat tcctgaaagc
     2101 gacagatgag ctgattgccg ccgttacagc acactggcgg gaagatttca ccgttctgcg
     2161 gctacatgga gactgccacg ccgggaatat tctctggcgc gatggcccaa tgtttgttga
     2221 tctggatgat gcacgtaatg gtccggctat tcaggatttg tggatgttgc tcaatggcga
     2281 taaagccgag cagcggatgc aactggaaac tattattgaa gcttatgaag aatttagcga
     2341 gttcgacacc gctgaaatcg gactgattga acctttacgc gccatgcgtt tggtttatta
     2401 tcttgcctgg ctaatgcggc gttgggctga tcccgcgttc ccgaaaaatt tcccgtggtt
     2461 aaccggggaa gattactggc tacgacagac ggcgactttt atagaacagg caaaagttct
     2521 tcaagaaccc cctttgcaat taacacctat gtattaatcg gagagagtag atcatgaaaa
     2581 agatttggct ggcgctggct ggtttagttt tagcgtttag cgcatcggcg gcgcagtatg
     2641 aagatggtaa acagtacact accctggaaa aaccggtagc tggcgcgccg caagtgctgg
     2701 agtttttctc tttcttctgc ccgcactgct atcagtttga agaagttctg catatttctg
     2761 ataatgtgaa gaaaaaactg ccggaaggcg tgaagatgac taaataccac gtcaacttca
     2821 tgggtggtga cctgggcaaa gatctgactc aggcatgggc tgtggcgatg gcgctgggcg
     2881 tggaagacaa agtgactgtt ccgctgtttg aaggcgtaca gaaaacccag accattcgtt
     2941 cagcatctga tatccgcgat gtatttatca acgcaggtat taaaggtgaa gagtacgacg
     3001 cggcgtggaa cagcttcgtg gtgaaatctc tggtcgctca gcaggaaaaa gctgcagctg
     3061 acgtgcaatt gcgtggtgtt ccggcgatgt ttgttaacgg taaatatcag ctgaatccgc
     3121 agggtatgga taccagcaat atggatgttt ttgttcagca gtatgctgat acagtgaaat
     3181 atctgtccga gaaaaaataa ttcaatgtaa attacataaa gcccgtgaat attcacgg
//
LOCUS       DAHHTG010000253         3218 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.260, whole genome shotgun sequence.
ACCESSION   DAHHTG010000253 DAHHTG010000000
VERSION     DAHHTG010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3218)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3218)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3218
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.260"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            complement(<1..281)
                     /gene="ybgE"
                     /locus_tag="NBI74_004559"
     CDS             complement(<1..281)
                     /gene="ybgE"
                     /locus_tag="NBI74_004559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyd operon protein YbgE"
                     /protein_id="HCD1934767.1"
                     /translation="MSKIIATLYAVMDKRPLRALSFVMALLLAGCMFWDPSRFAAKTS
                     ELEIWHGLLLMWAVCAGVIHGVGFRPQKVLWQGFFCPLLADIVLIVGLI"
     gene            complement(281..394)
                     /gene="cydX"
                     /locus_tag="NBI74_004560"
     CDS             complement(281..394)
                     /gene="cydX"
                     /locus_tag="NBI74_004560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-I oxidase subunit CydX"
                     /protein_id="HCD1934768.1"
                     /translation="MWYFAWILGTLLACSFGVITALALEHVESGKAGQEDI"
     gene            complement(409..1548)
                     /gene="cydB"
                     /locus_tag="NBI74_004561"
     CDS             complement(409..1548)
                     /gene="cydB"
                     /locus_tag="NBI74_004561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HCD1934769.1"
                     /translation="MIDYEVLRFIWWLLVGVLLIGFAVTDGFDMGVGMLTRFLGRNDT
                     ERRIMINSIAPHWDGNQVWLITAGGALFAAWPMVYAAAFSGFYVAMILVLASLFFRPV
                     GFDYRSKIEETRWRNMWDWGIFIGSFVPPLVIGVAFGNLLQGVPFNVDEYLRLYYTGN
                     FFQLLNPFGLLAGVVSVGMIITQGATYLQMRTVGELHLRTRATAQVAALVTLVCFALA
                     GVWVMYGIDGYVVKSTMDHYAASNPLNKEVVREAGAWLVNFNNTPILWAIPALGVVLP
                     LLTILTARMDKAAWAFVFSSLTLACIILTAGIAMFPFVMPSSTMMNASLTMWDATSSQ
                     LTLNVMTWVAVVLVPIILLYTAWCYWKMFGRITKEDIERNTHSLY"
     gene            complement(1564..3132)
                     /gene="cydA"
                     /locus_tag="NBI74_004562"
     CDS             complement(1564..3132)
                     /gene="cydA"
                     /locus_tag="NBI74_004562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415261.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HCD1934770.1"
                     /translation="MLDIVELSRLQFALTAMYHFLFVPLTLGMAFLLAIMETVYVLSG
                     KQIYKDMTKFWGKLFGINFALGVATGLTMEFQFGTNWSYYSHYVGDIFGAPLAIEGLM
                     AFFLESTFVGLFFFGWDRLGKVQHMCVTWLVALGSNLSALWILVANGWMQNPIASDFN
                     FETMRMEMVSFSELVLNPVAQVKFVHTVASGYVTGAMFILGISAWYMLKGRDFAFAKR
                     SFAIAASFGMAAVLSVIVLGDESGYEMGDVQKTKLAAIEAEWETQPAPAAFTLFGIPD
                     QEEETNKFAIQIPYALGIIATRSVDTPVIGLKELMVQHEERIRNGMKAYSLLEQLRSG
                     STDQAVRDQFNSMKKDLGYGLLLKRYTPNVADATEAQIQQATKDSIPRVAPLYFAFRI
                     MVACGFLLLAIIALSFWSVIRNRIGEKKWLLRAALYGIPLPWIAVEAGWFVAEYGRQP
                     WAIGEVLPTAVANSSLTAGDLIFSMVLICGLYTLFLVAELFLMFKFARLGPSSLKTGR
                     YHFEQSSTTTQPAR"
BASE COUNT          893 a          890 c          804 g          631 t
ORIGIN      
        1 aaaatcagcc cgacaatgag aacaatatcg gcaagcaatg ggcaaaaaaa cccttgccag
       61 agaaccttct gcggacgaaa gcccacgccg tgaatcacac cagcacagac ggcccacata
      121 agcagtaaac catgccagat ttccagttca ctggtcttcg cggcgaaacg tgatgggtcc
      181 caaaacatac atcctgctaa cagaagtgcc atcacgaagg aaagcgcccg cagggggcgc
      241 ttgtccatta ccgcatacaa ggttgcgata atcttactca tcagatgtct tcttgaccgg
      301 ctttgcctga ttcgacgtgc tcaagcgcca gcgcggtgat taccccaaac gaacaggcaa
      361 gaagcgttcc cagaatccat gcgaaatacc acatttttag ctccttactt agtacagaga
      421 gtgggtgtta cgttcaatat cttctttggt gatacgaccg aacattttcc agtaacacca
      481 ggcggtgtag agcagaatga tcggtaccag aaccaccgca acccaggtca tgacgttaag
      541 cgtcagctgg ctggaagttg catcccacat tgtcagactt gcgttcatca tggtgctgga
      601 cggcatcaca aacgggaaca ttgcgatacc ggcagtcagg atgatgcagg ccagcgtcag
      661 ggaggagaaa acaaacgccc acgcggcttt atccatacgt gcagtcagga tggtcagcag
      721 cggcagaacc acacccagtg ccggaatagc ccacagaatt ggcgtgttgt tgaagttaac
      781 cagccatgcg ccagcttcac gaaccacttc tttattcagc gggttagagg ctgcgtaatg
      841 gtccattgtc gatttcacga cataaccatc gataccgtac atcacccata cgccagccag
      901 tgcgaagcag accagcgtca ccagtgccgc cacctgagcc gttgcacggg tacgcaggtg
      961 cagttcgccc acggtacgca tttgcagata ggttgcgccc tgagtaatga tcatccctac
     1021 gctcaccaca cctgccagca ggccgaacgg gttaagcagc tggaagaagt taccagtgta
     1081 gtacagacgc agatattcat caacgttgaa tggtacgccc tgcaacaggt taccgaacgc
     1141 tacaccaatc accagcggcg gaacgaagct accaatgaag atgccccagt cccacatgtt
     1201 acgccagcgg gtttcttcaa tcttggagcg gtagtcaaaa ccgaccggac ggaagaacaa
     1261 agacgccagt acgaggatca tcgccacata gaagccggag aacgcagcgg catagaccat
     1321 cggccaggca gcaaacagtg caccgcctgc ggtgatcagc caaacctggt taccgtccca
     1381 gtgtggtgcg atggagttaa tcataattcg acgctcggtg tcgttacgac cgaggaaacg
     1441 ggtgagcatg cccaccccca tgtcgaaacc gtcagtgact gcaaaaccaa tcagcagaac
     1501 gccaaccagc agccaccaga taaaacgcaa tacttcataa tcgatcattt gacgactcct
     1561 gtcttagcgt gccggctgag tagtcgtgga agactgctca aagtgatagc gaccggtttt
     1621 caggctgctt gggccgaggc gtgcaaactt gaacattaag aacaattctg ccaccaggaa
     1681 cagggtatac aggccgcaaa tcagcaccat tgagaagatg agatcgcctg cggtcagtga
     1741 cgagttcgcc acagctgtcg gcagcacttc accgatagcc cacggttggc ggccatattc
     1801 agccacgaac cagcccgctt ctacagcaat ccacggcagc ggaataccgt acagcgcggc
     1861 gcgcagcagc cattttttct cgccaatgcg gttgcggatg acactccaga aagagagcgc
     1921 aatgattgcc agcagcagga agccacacgc caccatgata cggaacgcga agtacagcgg
     1981 cgctacacgc ggaatggagt ctttggttgc ctgttgaatc tgagcttcag tcgcatcagc
     2041 cacgtttggc gtatagcgtt tcagcagcag accgtaaccg agatctttct tcatgctatt
     2101 gaactggtca cgaacggcct ggtcagtaga accagaacgc agttgttcga gcagagagta
     2161 cgccttcatc ccgttacgaa tgcgttcttc atgctgcacc atcagttctt tcagaccgat
     2221 aactggagta tccacggaac gcgttgcaat gatgcccagc gcgtaaggga tctggatcgc
     2281 aaatttgttc gtctcctctt cctgatcagg aatgccgaac agagtaaagg cagcaggtgc
     2341 aggttgcgtt tcccactcgg cttcaatagc agccagtttg gttttctgca cgtcgcccat
     2401 ttcgtagccg gattcatcac ccagaacaat aacagacaga acggcagcca taccgaagct
     2461 ggcagcgata gcaaaggagc gtttagcgaa ggcgaagtca cgacctttca gcatatacca
     2521 tgcgctgata ccgaggatga acatcgcgcc agtcacataa ccagaagcta cggtgtgaac
     2581 gaatttcacc tgagcaaccg ggttaagcac cagctcggag aagctcacca tctccatacg
     2641 catagtttca aagttgaaat cggacgcgat tgggttttgc atccagccgt ttgcaaccag
     2701 aatccacagt gcggacaggt ttgaaccgag cgccaccagc caggtgacac acatatgctg
     2761 aactttaccc agacgatccc aaccgaagaa gaacagacct acaaaggtgg attcgaggaa
     2821 gaaggccatc agaccttcga ttgccagcgg cgcaccgaag atatccccta catagtggga
     2881 atagtaagac cagttagtcc cgaactggaa ctccatggtc agaccggtag ccacacccag
     2941 agcaaagttg ataccaaaca acttgcccca gaacttggtc atatctttat aaatctgttt
     3001 gccggaaagg acgtagaccg tttccataat ggccagcagg aacgccatac cgagcgtcag
     3061 tggcacaaaa aggaagtggt acatcgcggt caaggcaaac tgtaagcgcg acagttcgac
     3121 tatatctaac atcatgactc cttgctcatc gcatgaagac tccgagagtg aaccccgtta
     3181 gaaagggtca cacgcatgcc ccaatacaaa attatttg
//
LOCUS       DAHHTG010000254         3176 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.261, whole genome shotgun sequence.
ACCESSION   DAHHTG010000254 DAHHTG010000000
VERSION     DAHHTG010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3176)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3176)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3176
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.261"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            <1..688
                     /locus_tag="NBI74_004563"
     CDS             <1..688
                     /locus_tag="NBI74_004563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000039778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="inverse autotransporter beta domain-containing
                     protein"
                     /protein_id="HCD1934771.1"
                     /translation="EAVEITTKAIKQKITPSDIYRHALSGNILLSVYFQSPVILKKIQ
                     TFNGKIKFRQFVGDLLDKLCMLDRDGFIYGQNLRLCTEARYICPVQQIIDTPLLRKLN
                     QFRTFVRNVRPGDELDVQAQVSEKNLTPPPGNSSGNLEQQIASTSQLIGSLLAEDMNS
                     EQAANIARGWASSQASGVMTDWLSRFGTARITLGVDEDFSLKNSRDGNPRKWRFDATN
                     SELCAERHEC"
     gene            615..2737
                     /locus_tag="NBI74_004564"
                     /pseudo
     CDS             615..2737
                     /locus_tag="NBI74_004564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416485.4"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
     gene            complement(2907..>3176)
                     /locus_tag="NBI74_004565"
     CDS             complement(2907..>3176)
                     /locus_tag="NBI74_004565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HCD1934772.1"
                     /translation="NNVYRLSLSPSIKRSAAIIKDAYIAITPDTSILHMASAYNTPVV
                     AIYADYKTRWPAMADVSESVVVGQKIDNISLDEFAKALKSVLARI"
BASE COUNT          940 a          739 c          752 g          745 t
ORIGIN      
        1 ggaagcagtt gagatcacta ccaaagccat aaaacaaaaa attactccta gcgatattta
       61 tcgtcatgcc ttgagtggca acatcctact atcggtctat ttccagtctc cagtgatact
      121 taagaagata caaactttta atggaaaaat aaaattcagg cagtttgtgg gagacctact
      181 tgataaacta tgcatgcttg acagggacgg ttttatttat ggtcaaaatt taagactctg
      241 tacagaagcc agatatattt gtcctgtgca acaaattata gatacccccc tgttacgcaa
      301 actcaaccag tttcgtacgt ttgttcgtaa tgtccgcccg ggtgatgaac tggatgtcca
      361 ggcacaagtt agtgaaaaaa atttaacccc gccgccgggt aacagcagtg gcaaccttga
      421 gcaacagata gccagtactt cacagctaat tggatctctg ctcgccgaag atatgaacag
      481 cgagcaagcg gcaaacatag cacgtggatg ggcctcttct caggcttcag gggtaatgac
      541 agactggtta agccgcttcg gtaccgcaag aatcacgctg ggcgtggatg aagatttcag
      601 cctgaagaac tcccgtgacg gcaaccctcg aaaatggcga ttcgatgcaa caaacagtga
      661 actatgtgcc gaacgtcacg aatgctgaaa tcacgctggc agcctcgaag gatccggtga
      721 ttgccgacaa taacgatctc acgacactaa cagcaccgtc gctgatacag agggcaatgc
      781 gatagccaac actgaggtaa catttactct accggaagat gtgaaggcga acttcacgct
      841 gagcgatggc gacaaagcgg ttactgatgc cgatggcaaa gcgaaagtca cgctgaaagg
      901 tacaaaagca ggcgctcata ccgtgacagc atcgatggtt ggcggtaaga gtgagcagtt
      961 ggtggtgaac tttactgcgg atacgctcac tgcgcaggtt aatcttaacg ttaccgagga
     1021 caatttcatc gccaataaca ttggaatgac caggctacag gcaacagtga ctgatggaaa
     1081 cggcaaccca gtagaaggta taaaagttaa tttccgcgga acctccgtca cgctaagcag
     1141 caccagcgtt gaaacggatg atcaggtttt cgctgaaatt cttgtgacaa gcaccgaggt
     1201 cggactaaaa acagtttcag cctctctggc agataaacct actgaagtca tctcgcgatt
     1261 actgaatgcc aaggtagatg ttaattctgc gacgattacc agtcaggaga tacctgaagg
     1321 tcaggtcatg gtcgcacaag acatagcagt taaagctcac gttaacgacc agtttggcaa
     1381 cccggtgacg catcaacccg cgacattcag tgcagcgcca tcctcgcaaa tgatcatcag
     1441 ccagaatacg gtctctacta atacgcaggg agtagccgag gtcaccataa cgcctgaaag
     1501 aaacggctcg tatactgtaa aagcatccct agcgaatgga gcttcacttg agaaacaact
     1561 ggaggctatt gatgaaaaac taacactcac gtccagtccg cttatcggtg tcaatgctcc
     1621 taaaggcgct actctgacgg caacgctaac ctctgcaaat ggcactccag tggagggtca
     1681 ggtcatcaac tttagcgtaa cgctagaagg tgcgacgtta agtggcggaa aagtgagaac
     1741 caactcttca ggtcaggctc cagtcgttct gaccagcaat aaagtcggta catatacggt
     1801 gactgcatcg ttccataacg gcgtcacaat acagacacag acaaccgtga aggttactgg
     1861 caacccaagc accgcacatg ttgctagctt tatcgctgat ccgtcgacta tcaccgccac
     1921 caacagtgat ttaagtacct taaaggcaac ggttgaggat ggctgtggta acctgatcga
     1981 aggtctcact gtgtacttcg ccttaaaaag cggctccacc acattaacgt cattaacagc
     2041 ggtgaccgat caaaacggaa tcgcgacaac aagcgtgaaa ggagcgataa cggggagcgt
     2101 cactgtaagc acagtcacaa gcgcaggtgg aatgcaaaca gtagatatat cgctagtggc
     2161 aggcccagca gacgcctcgc agtccatcct taagaacaac cagtcatcac tgaaagggga
     2221 ctttaccgat agtgctgagc tacatcttgt tctgcacgat atatcaggca atccgatcaa
     2281 agtttcttaa gggatagaat ttgtgcaatc aggtacaaac gtgccctata tgaaaattag
     2341 cgcaattgat tacagtcaaa atatcaacgg tgattacaaa gctactgtta caggaggcgg
     2401 agagggtatc gcaacgctga tccccgtatt gaatggtgtt catcaggcgg gtctgagtac
     2461 cacgatagaa ttcattagcg cggaaaccag accaatgacg ggtacagtgt cagtgaatag
     2521 tgcaaaccta ccgacagctt cattcccttc gcagggattt accggggcgt attatcagtt
     2581 gaataatgac aactttgcac caggaaaaac ggcggctgat tattcgtttt caagctcggc
     2641 ctcctgggta ggcgttgatg ctactggtaa agtgacattc aaaaatgacg gtgatagcaa
     2701 tactgtaata atcacagcgc ccccaagatc cggtggtgct atttaccaaa cagttcctcc
     2761 tgaatcgcgt tcggtgtgac tatgatcatt ttcggtgagt attgctttga tccaaaggct
     2821 ggttttctgt gatgattgac cagcctcaga gtattgatga caccaccgtt cataacagat
     2881 cggcattact atgcataagt actgcctcaa attctcgcca aaacactttt taatgccttt
     2941 gcgaattcat ccagacttat attgtcaatt ttttgcccaa cgacgactga ctccgaaaca
     3001 tcggccattg cgggccatcg cgttttgtaa tcagcataaa ttgcaacaac aggggtatta
     3061 taggcacttg ccatatgtaa tattgaggtg tcaggagtta ttgcaatata agcatctttt
     3121 atgattgctg ctgaacgttt aatcgagggt gataaggata aacggtagac attatt
//
LOCUS       DAHHTG010000255         3104 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.262, whole genome shotgun sequence.
ACCESSION   DAHHTG010000255 DAHHTG010000000
VERSION     DAHHTG010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3104)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3104)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3104
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.262"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            4..231
                     /locus_tag="NBI74_004566"
     CDS             4..231
                     /locus_tag="NBI74_004566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004201115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCD1934773.1"
                     /translation="MTNQTIQLAISITGSQKRLADLCGVAQPTVWRWLHGGGIDARYV
                     MKIVSATGGKIKPADIRPDLAPLFNASNSAA"
     gene            262..801
                     /locus_tag="NBI74_004567"
     CDS             262..801
                     /locus_tag="NBI74_004567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001182892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin YdaT domain-containing protein"
                     /protein_id="HCD1934774.1"
                     /translation="MQPLTYQQTSGFSPTAVINRSQTKQAPGHEKIRDAVRAWSAADN
                     QDVVAALIVNEYREQGGGTIDFPDDVSRARQKLFRFLDNKFDSEKYRNNVRELTPAIL
                     AVLPLEYRGHLVEQDSFMARLAEMEKELSEAKQAVILNAPRHQKLKEMSEGIVSMFRV
                     DPDLAGPLMAMVTTMLGAI"
     gene            798..1817
                     /locus_tag="NBI74_004568"
     CDS             798..1817
                     /locus_tag="NBI74_004568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000147908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HCD1934775.1"
                     /translation="MTGSEMAKAGLREQNRLSGANRNTLIAGGIMANTAEIFNFPVPD
                     VAQKEPRVADLDDGYTRIANELLEAVMLAGLTQHQLLVFLAVMRKTYGFNKKLDWVSN
                     EQLSELTGILPHKCSAAKSVLVKRGIFIQSGRNIGINNVVSEWSTLPESGKKNKVYLK
                     EVNLPESGKKSLPKSGKGVYPNQVNTKDKLTKDNIKPFSSENSDESSDQPENDLPVVK
                     PDAAIQSGSKWGTAEDLTAAEWMFDMVKTIAPSARKPNFAGWANDIRLMRERDGRNHR
                     DMCVLFRWACQDNFWSGNVLSPAKLRDKWTQLEINRNKQQAGVTAGKSKLDLTNTDWI
                     YGVDL"
     gene            1814..2515
                     /locus_tag="NBI74_004569"
     CDS             1814..2515
                     /locus_tag="NBI74_004569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Replication protein P"
                     /protein_id="HCD1934776.1"
                     /translation="MKNIAAQMVNFDREQMRRIANNMPEQYDEKPQVQQVAQIINGVF
                     SQLLATFPASLANRDQNELNEIRRQWVLAFRENGITTMEQVNAGMRVARRQNRPFLPS
                     PGQFVAWCREEASVIAGLPNVSELVDMVYEYCRKRGLYPDAESYPWKSNAHYWLVTNL
                     YQNMRANALTDAELRRKAADELVHMTARINRGEAIPEPVKQLPVMGGRPLNRAQALAK
                     IAEIKAKFGLKGASV"
     gene            2512..2814
                     /locus_tag="NBI74_004570"
     CDS             2512..2814
                     /locus_tag="NBI74_004570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein ren"
                     /protein_id="HCD1934777.1"
                     /translation="MTGKEAIIHYLGTHKSFCAQDVAAVTGATVTSINQAVAKMARAG
                     ILVVDGKVWRTVYYRFATREEREGKVSANLIFKECRQSAAMKRVLRVYKRTSMGTQ"
     gene            2882..>3104
                     /gene="emrE"
                     /locus_tag="NBI74_004571"
     CDS             2882..>3104
                     /gene="emrE"
                     /locus_tag="NBI74_004571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMR family multidrug efflux protein EmrE"
                     /protein_id="HCD1934778.1"
                     /translation="MNPYIYLGGAILAEVIGTTLMKFSEGFTRLWPSVGTIICYCASF
                     WLLAQTLAYIPTGIAYAIWSGVGIVLISLL"
BASE COUNT          870 a          705 c          829 g          700 t
ORIGIN      
        1 ataatgacaa atcaaaccat tcaactcgca atcagtatta caggtagtca aaaacgactg
       61 gcagatctat gcggtgtagc ccagcccact gtttggcgtt ggctacacgg tggcggaatt
      121 gatgcccgct atgtaatgaa aattgtctca gccactggtg gaaagattaa accagcagat
      181 attcgtcccg acctcgcacc attgtttaac gcgagtaatt ctgccgccta atctgcggcg
      241 ttaactgata aggcaatgac tatgcaacca cttacatacc aacagactag cggatttagc
      301 ccgactgcgg tgataaatcg ttctcaaaca aaacaggcgc caggccacga aaaaatccgt
      361 gatgccgttc gcgcctggtc ggctgcagat aatcaggatg ttgttgccgc actcattgtg
      421 aatgagtatc gggagcaggg cggcggcacc atcgattttc ctgatgatgt cagccgtgca
      481 cgccagaagc tgttccgctt cctcgataac aaattcgatt ctgaaaaata ccgaaataac
      541 gtgcgtgaac tgaccccggc aattctggcg gtactaccgc tggaatatcg cggccacctg
      601 gttgagcagg atagcttcat ggctcggctg gctgaaatgg aaaaggaact cagtgaggca
      661 aaacaggctg tcattctcaa cgcaccacgc caccagaaac tgaaggagat gagtgaaggc
      721 attgtgtcga tgtttcgtgt ggacccggat ctggctggtc cactgatggc gatggtcacc
      781 accatgctgg gggcaatatg acaggttcgg aaatggcgaa agccggtctg cgcgaacaga
      841 accgactttc aggtgcaaat cgtaacacac tcattgcggg aggaattatg gcaaacactg
      901 ctgagatatt caattttcca gtgccggatg tggcacaaaa ggagccgcgc gtggcagatc
      961 tcgatgatgg ttatacgcgc attgcaaatg agttgctgga agctgtgatg ctggccggat
     1021 taacacagca ccagcttctg gtcttcctgg ctgtcatgcg caaaacatat ggctttaata
     1081 aaaaactgga ttgggtgagc aacgagcaac tttccgagtt gaccgggata ttgccgcaca
     1141 agtgttctgc tgcaaaaagt gttctggtaa agcgtgggat ttttattcag agcgggcgga
     1201 atatcggcat taataatgtg gtcagtgaat ggtcaacatt acccgaatca ggtaagaaaa
     1261 ataaagttta cctgaaagag gtaaatttac ctgaatcagg taagaaaagt ttacccaaat
     1321 caggtaaagg cgtttacccg aatcaggtaa acacaaaaga caaactaaca aaagacaata
     1381 taaaaccttt ttcgtccgag aattctgacg aatcctctga ccagccagaa aacgatcttc
     1441 ctgtggtgaa accggatgct gcaattcaga gcggcagcaa gtgggggaca gcagaagacc
     1501 tgaccgccgc agagtggatg tttgacatgg tgaagaccat cgcgccatca gccagaaaac
     1561 caaattttgc agggtgggct aacgatatcc gcctgatgcg tgaacgtgac ggacgtaacc
     1621 accgcgacat gtgcgtgctg ttccgctggg catgccagga caacttctgg tccggtaacg
     1681 tgctgagtcc ggccaaactc cgcgacaagt ggactcaact cgaaatcaac cgtaacaagc
     1741 aacaggctgg cgtgacagcc ggaaaatcaa aactcgacct gacaaacact gactggattt
     1801 acggggtgga tttatgaaaa acatcgccgc acagatggtt aactttgacc gtgagcagat
     1861 gcgccggatc gccaacaaca tgccggaaca gtacgacgaa aagccgcagg tacagcaggt
     1921 agcgcagatc atcaacggtg tgttcagcca gttactggca actttcccgg cgagtctggc
     1981 taaccgtgac cagaacgaac tgaacgaaat ccgccgccag tgggttctgg ctttccggga
     2041 aaacgggatc accacaatgg aacaggttaa cgcaggaatg cgcgtagccc gtcggcagaa
     2101 tcgaccattt ctgccatcac ccgggcagtt tgttgcatgg tgccgggaag aagcatccgt
     2161 tatcgccgga ctgccaaacg tcagcgagct ggttgatatg gtttacgagt attgccggaa
     2221 gcgaggcctg tatccggatg cggagtctta tccgtggaaa tcaaacgcgc actactggct
     2281 ggttaccaac ctgtatcaga acatgcgggc caatgcgctt actgatgcgg aattacgccg
     2341 taaggccgca gatgagcttg tccatatgac tgcgagaatt aaccgtggtg aggcgatccc
     2401 tgaaccagta aaacaacttc ctgtcatggg cggtagacct ctaaatcgtg cacaggctct
     2461 ggcgaagatc gcagaaatca aagctaagtt cggactgaaa ggagcaagtg tatgacgggc
     2521 aaagaggcaa ttattcatta cctggggacg cataagagct tctgtgcaca ggacgttgcc
     2581 gcggtaacag gcgcaaccgt aaccagcata aatcaggctg tggctaaaat ggcgcgggca
     2641 ggaatcctgg tcgttgatgg taaggtctgg cgaacggtgt attatcggtt cgctaccaga
     2701 gaagaacggg aaggaaaggt gagcgcgaat ttgattttta aggagtgtcg ccagagtgcc
     2761 gcgatgaaac gggtattgag ggtatataaa agaacatcaa tgggtacaca atgatgaaac
     2821 aggtgagttg agttcaaact gtagtacaat tctctccagt ttgaacagga aagaatatgc
     2881 tatgaaccct tatatttatc ttggtggtgc aatacttgca gaggtcattg gtacaacctt
     2941 aatgaagttt tcagaaggtt ttacacggtt atggccatct gttggtacaa ttatttgtta
     3001 ttgtgcatca ttctggttat tagctcagac gctggcttat atacctacag ggattgctta
     3061 tgctatctgg tcaggagtcg gtattgtcct gattagctta ctat
//
LOCUS       DAHHTG010000256         3086 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.263, whole genome shotgun sequence.
ACCESSION   DAHHTG010000256 DAHHTG010000000
VERSION     DAHHTG010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3086)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3086)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3086
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.263"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            64..891
                     /gene="mepH"
                     /locus_tag="NBI74_004572"
     CDS             64..891
                     /gene="mepH"
                     /locus_tag="NBI74_004572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-endopeptidase MepH"
                     /protein_id="HCD1934779.1"
                     /translation="MARINRISITLCALLFTTLPLTPMAHASKQARESSATTHITKKA
                     DKKKSTATTQKTQKASQKTAKKAASKSTTKSKTASSVKKSSITASKNAKTRSKHAVNK
                     TASASFTEKCTKRKGYKSHCVKVKNAASGTLADAHKAKVQKATKVAMNKLMQQIGKPY
                     RWGGSSPRTGFDCSGLVYYAYKDLVKIRIPRTANEMYHLRDAAPIERSELKNGDLVFF
                     RTQGRGTADHVGVYVGNGKFIQSPRTGQEIQITSLSEDYWQRHYVGARRVMTPKTLR"
     gene            1019..1600
                     /gene="sodB"
                     /locus_tag="NBI74_004573"
     CDS             1019..1600
                     /gene="sodB"
                     /locus_tag="NBI74_004573"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Fe]"
                     /protein_id="HCD1934780.1"
                     /translation="MSFELPALPYAKDALAPHISAETIEYHYGKHHQTYVTNLNNLIK
                     GTAFEGKPLEEIIRSSEGGVFNNAAQVWNHTFYWNCLAPNAGGEPTGKVAEAIAASFG
                     SFADFKAQFTDAAIKNFGSGWTWLVKNSDGKLAIVSTSNAGTPLTTDATPLLTVDVWE
                     HAYYIDYRNARPGYLEHFWALVNWEFVAKNLAA"
     gene            complement(1746..2915)
                     /locus_tag="NBI74_004574"
     CDS             complement(1746..2915)
                     /locus_tag="NBI74_004574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCD1934781.1"
                     /translation="MKINYPLLALAIGAFGIGTTEFSPMGLLPVIARGVDVSIPAAGM
                     LISAYAVGVMVGAPLMTLLLSHRARRSALIFLMAIFTLGNVLSAIAPDYMTLMLSRIL
                     TSLNHGAFFGLGSVVAASVVPKHKQASAVATMFMGLTLANIGGVPAATWLGETIGWRM
                     SFLATAGLGVISMVSLFLSLPKGGAGARPEVKKELAVLMRPQVLSALLTTVLGAGAMF
                     TLYTYISPVLQSITHVTPVFVTAMLVLIGVGFSIGNYLGGKLADRSVNGTLKGFLLLL
                     MVIMLAIPFLARNEFGAAISMVVWGAATFAIVPPLQMRVMRVASEAPGLSSSVNIGAF
                     NLGNALGAAAGGAVISAGLGYSFVPVMGAIVAGLALLLVFMSARKQPEAVCVANS"
BASE COUNT          895 a          835 c          704 g          652 t
ORIGIN      
        1 aatcatcaag ttttgtaaat cagacgcagg catgatagac ctgccttaac agagggacgc
       61 tcagtggcgc ggataaaccg tatttcgatc acgctctgtg ctttactttt taccaccctg
      121 cctttaacgc ctatggccca tgcttcaaag caagccaggg agagttctgc taccactcat
      181 atcaccaaaa aagcagataa aaagaaaagc acggcaacca cccaaaagac ccagaaagcc
      241 agccagaaaa cagcgaaaaa agccgccagt aaaagtacga ccaaaagcaa aaccgcttct
      301 tccgttaaaa aatcttccat taccgcttct aaaaacgcca aaactcgcag caaacacgcc
      361 gtcaataaaa cggcctcagc cagctttacc gaaaagtgta ccaaacgtaa gggctataaa
      421 tcgcattgtg tgaaagtcaa aaatgccgcg tcaggaaccc ttgccgacgc gcacaaagcg
      481 aaggtgcaaa aagccacaaa agtggcaatg aataaactga tgcagcaaat tggtaagcca
      541 tatcgttggg gtggcagctc accgcgtacc ggttttgatt gcagcggtct ggtttattac
      601 gcttataaag atttggtgaa aattcgtatt ccgcgtacgg cgaatgaaat gtatcacctg
      661 cgcgatgcag cgccaatcga acgtagtgaa ctgaaaaacg gcgacctggt cttcttccgt
      721 actcagggac gcggtacagc cgatcatgtc ggcgtgtatg tcggcaacgg caaatttatt
      781 cagtcaccgc gcacgggcca ggaaattcaa atcacttctc tcagtgaaga ctactggcag
      841 cgccactatg ttggcgctcg ccgggtaatg accccaaaaa cacttcgcta aaactttacc
      901 ctgttgttac ggcaacaggg taagttcatc ttttgtctca ccttttaatt tgctacccta
      961 tccatacgca caataaggct attgtacgta tgcaaattaa taataaagga gagtagcaat
     1021 gtcattcgaa ttacctgcac taccatatgc taaagatgct ctggcaccgc acatttctgc
     1081 ggaaaccatc gagtatcact acggcaaaca tcatcagact tatgtcacta acctgaacaa
     1141 cctgatcaaa ggtaccgcgt ttgaaggtaa accactggaa gagattattc gcagctctga
     1201 aggtggcgta ttcaacaacg cagctcaggt ctggaaccat actttctact ggaactgcct
     1261 ggcaccgaac gcgggtggcg aaccgactgg aaaagtcgct gaagctatcg ccgcatcttt
     1321 tggcagcttt gccgatttca aagctcagtt tactgatgca gcgatcaaaa actttggttc
     1381 tggctggacc tggctggtga aaaacagcga tggcaaactg gctatcgttt caacctctaa
     1441 cgcgggtact ccactgacca ccgatgcgac tccgctgctg accgttgatg tctgggaaca
     1501 cgcttattac atcgactatc gcaatgcacg ccctggctat ctggagcact tctgggcgct
     1561 ggtgaactgg gaattcgtag cgaaaaatct cgctgcataa taactgatgg caaatgcaac
     1621 attacctgat gcgcttcgct taccatgcca acgcggatga ggcgttaaca gcgcattcgg
     1681 caagataaat cgcacgttgc cagcaactgt aacgcagaag gttatccttc tgcgtttttg
     1741 tttaattagc tgttggcaac gcaaactgct tcaggttgtt ttctggctga cataaacacc
     1801 agcaataatg ccagtcccgc gacaatcgcc cccatcaccg gcacaaaact gtatcccagc
     1861 ccagcggaaa ttaccgcacc accagcagct gctcccagcg catttccaag attaaaggca
     1921 ccaatattga ctgatgaaga cagacctggc gcttcactgg cgacacgcat cacgcgcatc
     1981 tgtaacggcg gtacgatcgc aaaggttgca gctccccaca ccaccatgct aatagctgcg
     2041 ccgaactcat tgcgggccag gaacgggatt gccagcataa tcaccatcag caacaacaaa
     2101 aagcctttca acgtgccgtt aactgaacga tctgccagtt tgccgccgag atagttaccg
     2161 atagagaatc cgacaccaat cagcaccagc attgccgtga cgaacaccgg cgttacgtgg
     2221 gtaatgcttt gcagtaccgg agagatatag gtgtagaggg taaacattgc accagcgccc
     2281 agtaccgtcg tcagcaatgc agacagcacc tgcggacgca ttaataccgc cagctctttt
     2341 ttcacttcag gtcgcgcccc tgcgccacct ttaggtaatg agaggaacag acttaccatt
     2401 gaaatcacgc ccagccccgc tgttgccaga aatgacatcc gccagccgat ggtttcgccc
     2461 aaccaggtcg ccgccggcac gccaccgata tttgccaggg ttaaccccat aaacatagtg
     2521 gcaactgcgc tggcctgttt atgttttggc actacgcttg cggccacgac tgaacccaaa
     2581 ccaaaaaatg ccccgtgatt caggctggtc aaaatgcgtg aaagcatcag ggtcatataa
     2641 tccggcgcga tggcggaaag tacgttgcca agcgtgaaaa tcgccatcag gaaaatcaac
     2701 gcactgcggc gggcacgatg agaaagtaga agcgtcatca gcggcgcgcc aaccattacg
     2761 ccaactgcat aagcactgat taacattccg gcagcgggaa tcgagacatc cacaccgcgc
     2821 gcaatgacgg gcaacaagcc cattggcgag aactccgttg tcccgatacc aaacgcgcca
     2881 atcgccagcg ccagcaacgg atagttaatt ttcatgcctt atctccacct cttcgcgtca
     2941 ttacgcgata ttcattaaag tggcgaaagc atgacagtaa tcacaaaaaa atgaaaataa
     3001 caaaaagaga aaacactttt gccattttgc taacaaacag gaaggagatg cgagggagaa
     3061 cgcgctccct cgagaggaaa tcagtg
//
LOCUS       DAHHTG010000257         3036 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.264, whole genome shotgun sequence.
ACCESSION   DAHHTG010000257 DAHHTG010000000
VERSION     DAHHTG010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3036)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3036)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3036
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.264"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            complement(72..845)
                     /locus_tag="NBI74_004575"
     CDS             complement(72..845)
                     /locus_tag="NBI74_004575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiO family outer membrane beta-barrel protein"
                     /protein_id="HCD1934782.1"
                     /translation="MIKHTLLVPFFFSALPAYAGLTSITAGYDFTDYSGEHGNRNLAY
                     AELVAKVENATLLFNLSQGRRDYETEHFNATRGQGAVWYKWNNWLTTRTGIAFADNTP
                     VFARQDFRQDINLALLPKTLFTTGYRYTKYYNDVEVDAWQGGISLYTGPVITSYRYTH
                     YDSSDAGSSYSNMISVRLKDPRGAGYTQLWLSRGTGAYTYDWTPETRYGSMKSISLQR
                     IQPLTEQLNLGLTAGKVWFNTPTDDYNGLQLAAHLIWKF"
     gene            complement(847..1371)
                     /locus_tag="NBI74_004576"
     CDS             complement(847..1371)
                     /locus_tag="NBI74_004576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020231914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HCD1934783.1"
                     /translation="MIIDESAGEVVIGANTRICHGAVIQGPVVIGANCLIGNYAFIRP
                     GTIISNGVKIGFATEIKNAVIEAEATIGPQCFIADSVVANQAYLGAQVRTSNHRLDEQ
                     PVSVRTPEGIIATGCDKLGCYIGKRSRLGVQVIILPGRIISPNTQLGPRVIVERNLPS
                     GTYSLRQELIRTGD"
     gene            complement(1406..2602)
                     /locus_tag="NBI74_004577"
     CDS             complement(1406..2602)
                     /locus_tag="NBI74_004577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HCD1934784.1"
                     /translation="MKTLLFIFMTIAMLLWFLSTLRRKPCQKKGCIDAIIPAYNEGPC
                     LAQSLDNLLRNPYFCRVICVNDGSTDNTEAVMAEVKRKWGDRFVAVTQKNTGKGGALM
                     HGLKYATCDQVFLSDADTFVPPDNDGMGYMLAEIERGADAVGGIPSTALKGAGLLPHI
                     RATVKLPMIVMKRTLQQFLGGAPFIISGACGMFRTDVLRKFGFSDRTKVEDLDLTWTL
                     VANGYRIRQANRCIVYPQECNSPREEWRRWRRWIVGYAVCMRLHKRLLFSRFGIFSIF
                     PMLLVVLYGVGIYLTTWFNEFITTGPHGVVLAMFPLIWVGVVCVIGAFSAWFHRCWLL
                     VPLAPLSVVYVLLAYAIWIIYGLIAFFTGREPQRDKPTRYSALVEASTAYSQPSVTGT
                     EKLSEA"
     gene            complement(2616..>3036)
                     /locus_tag="NBI74_004578"
     CDS             complement(2616..>3036)
                     /locus_tag="NBI74_004578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001560584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HCD1934785.1"
                     /translation="NFEDTRLYLQIKEMISSLEYIIENETPQDVEITRVYTMNNTDRH
                     QDHIAVYQASMVACRYIPQILGYETPSTCLSFLPQVFESVDEVHFNKKILALQKHKSQ
                     IHRRYIQPEYIIAQAKFRGQQVSHSMCEGFVVHKMVL"
BASE COUNT          851 a          722 c          663 g          800 t
ORIGIN      
        1 tgtttattat caaaccgagt tatagctttt ctctgaaaac taccgaataa aatatactga
       61 tagactgctg cctagaattt ccagatcagg tgggctgcaa gttgcagacc gttataatca
      121 tcggttgggg tgttgaacca cactttacct gccgtcaacc cgagattaag ttgctcagtc
      181 agcggttgaa ttcgttgcaa actgatgctc ttcatgctgc cgtagcgtgt ttctggcgtc
      241 cagtcgtagg tgtaagcacc tgttccgcgg cttagccata gttgcgtata accagcaccg
      301 cgcgggtcct tcagacgcac ggaaatcata ttgctataac tactacctgc atcgctggag
      361 tcataatggg tatagcgata gctggtgatc accgggccag tatagagtga tatgccgcct
      421 tgccaggcat cgacttcgac atcattgtaa tatttagtgt agcgataacc ggtcgtgaaa
      481 agcgtttttg gcaacagggc caggttaata tcctgacgaa aatcctggcg ggcaaagacc
      541 ggcgtattat ccgcaaaggc aatacccgtt cgggttgtca gccagttatt ccatttgtac
      601 cagactgcac cctggccgcg agtggcattg aaatgttcag tttcataatc acgacgcccc
      661 tgggaaagat taaaaagcag cgttgcgttt tcaacttttg ctaccagttc agcatacgct
      721 aaattacggt tgccatgttc gccagaataa tcggtaaaat cgtagcccgc ggtaatggaa
      781 gttaacccgg cataagcggg caatgcacta aaaaaaaacg gcactaataa cgtatgcttt
      841 atcattttaa tctcctgtac ggataagttc ttgtcggagt gagtaagttc cactaggtaa
      901 attacgttct acaatcacgc gcgggccaag ttgtgtgttc ggagaaataa ttcgcccagg
      961 caaaataata acttgtacac caaggcgtga acgcttcccg atataacaac ctaatttatc
     1021 gcatccggta gcgataattc cctctggagt tcgaacagac acgggttgtt catccagacg
     1081 atgattactg gtacgtactt gtgcgcccaa atatgcctgg tttgcaacta ccgagtcggc
     1141 aataaagcat tgcggaccaa tcgttgcttc cgcttcaata accgcatttt taatttcggt
     1201 ggcaaaacca atttttacgc cattgctgat tattgtgcca ggacgaataa acgcataatt
     1261 acctatcagg cagtttgcgc caatcactac cggcccctga ataacggcac cgtggcaaat
     1321 acgggtattc gcgccgataa caacctcacc ggcactttca tcaataatca cctggtctgc
     1381 aatctgaaca tttttctctt tagcattaag cttcagatag tttttcagtt cctgtgacag
     1441 aaggttggga ataagcggtt gacgcttcca ccagtgcgga atagcgggtg ggtttgtcgc
     1501 gctgaggttc gcgtccagta aaaaaggcaa taagtccata aataatccag atggcataag
     1561 ctaataatac atacacaacg gaaagcggtg ctaaaggcac caacaaccag caacgatgaa
     1621 accaggcgct aaatgcacca ataacacaaa ctacgccgac ccagataagc gggaacattg
     1681 ccaacaccac accatgtggc ccggtggtga tgaattcatt aaaccaggta gtgagataaa
     1741 taccaacgcc ataaagcaca accaacagca taggaaatat actgaagata ccgaagcggc
     1801 taaataaaag tcttttatgc aggcgcatac agaccgcgta gcccacaatc caacgccgcc
     1861 agcggcgcca ctcctcacgc ggactgttac attcctgtgg gtatacgatg cagcgattcg
     1921 cctgccgaat acggtagccg tttgccacca acgtccaggt gagatcaagg tcttcgactt
     1981 tagtacgatc agagaaaccg aacttacgca atacatcagt acggaacatc ccgcaggcac
     2041 cgctgataat aaacggtgca ccgcccagaa actgctgtag cgtgcgcttc ataacaatca
     2101 tcggcaactt tacggtggcg cggatatgtg gcagcagacc tgcccctttt aacgcggtgg
     2161 agggaattcc gccaacggca tcagcgccgc gctcaatttc tgccagcata tagcccattc
     2221 catcattatc aggtggaacg aaagtatctg catcacttaa aaatacttga tcacacgtgg
     2281 cgtattttag tccatgcatt agtgcgccac ctttaccggt gtttttttgc gtgacggcaa
     2341 caaagcggtc gccccatttg cgtttgactt ccgccatgac cgcttcggta ttgtccgtgg
     2401 atccgtcgtt aacgcaaatt acccggcaaa aataagggtt acgcagcaga ttatccagtg
     2461 actgcgccag acacgggcct tcgttataag ctggtataat ggcgtcaata cagcctttct
     2521 tttgacaggg tttacgtctt agcgtactta aaaaccatag caacattgct atggtcatga
     2581 agataaataa caaagttttc atttctatta ctccgttaca acaccatctt atggacaaca
     2641 aaaccttcac acatagaatg actaacctgt tgtcctcgga atttggcttg ggcaattatg
     2701 tattctggct gtatgtatct tcgatgaatc tggcttttat gcttttgtaa tgctaatatt
     2761 tttttattaa aatggacctc atctacagat tcaaaaactt gaggtaaaaa tgacagacat
     2821 gtacttggtg tttcgtatcc tagtatttga ggaatataac gacaggcaac cattgatgcc
     2881 tgataaacag caatgtgatc ctgatgcctg tcagtattat tcatggtgta aactcgagta
     2941 atttccacat cctggggcgt ttcattctca attatgtatt ccagagatga tatcatctct
     3001 tttatttgca aatataatct ggtatcttca aaattt
//
LOCUS       DAHHTG010000258         2865 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.265, whole genome shotgun sequence.
ACCESSION   DAHHTG010000258 DAHHTG010000000
VERSION     DAHHTG010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2865)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2865)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2865
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.265"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            complement(375..975)
                     /locus_tag="NBI74_004579"
                     /pseudo
     CDS             complement(375..975)
                     /locus_tag="NBI74_004579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020237919.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
     gene            complement(1064..1498)
                     /gene="uspF"
                     /locus_tag="NBI74_004580"
     CDS             complement(1064..1498)
                     /gene="uspF"
                     /locus_tag="NBI74_004580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310024.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspF"
                     /protein_id="HCD1934786.1"
                     /translation="MNRTILVPIDISDSELTQRVISHVEAEAKIDDAEVHFLTVIPSL
                     PYYASLGLAYSAELPAMDDLKAEAKSQLEEIIKKFKLPTDRVHVHVEEGSPKDRILEL
                     AKKIPAHMIIIASHRPDITTYLLGSNAAAVVRHAECSVLVVR"
     gene            complement(1639..2772)
                     /gene="ompN"
                     /locus_tag="NBI74_004581"
     CDS             complement(1639..2772)
                     /gene="ompN"
                     /locus_tag="NBI74_004581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpN"
                     /protein_id="HCD1934787.1"
                     /translation="MKSKVLALLIPALLAAGAAHAAEVYNKDGNKLDLYGKVDGLHYF
                     SDNSAKDGDQSYARLGFKGETQINDQLTGYGQWEYNIQANNTESSTNQSWTRLAFAGL
                     KFADYGSFDYGRNYGVMYDIEGWTDMLPEFGGDSYTNADNFMTGRANGVATYRNTDFF
                     GLVNGLNFAVQYQGNNEGASNGQEGTNNGRDVRHENGDGWGLSTTYDLGMGFSAGAAY
                     TSSDRTNDQVNHTAAGGDKADAWTAGLKYDANNIYLATMYSETRNMTPFGDSDYAVAN
                     KTQNFEVTAQYQFDFGLRPAVSFLMSKGRDLHAAGGADNPAGVDDKDLVKYADVGATY
                     YFNKNMSTYVDYKINLLDEDDSFYAANGISTDDIVALGLVYQF"
BASE COUNT          846 a          594 c          633 g          792 t
ORIGIN      
        1 attagtttga tgattttata ctgaactaaa gggggaaaga ggtaacgaaa aaagacctcc
       61 accaggtacg aatctgaggc gggcagatgg aggtcgatat ctacccgatt aaatggataa
      121 gtaaaagccc catcagggat tgatttgttc gagtctaatt taaagtaatt tcaacgattt
      181 ggcagaactt cttagagtta atgattttca agttttcgat taccaggaaa aaggctgggg
      241 gctgataaaa tagtcatctg cattccttaa attgtgtaag ggtgaaagga taaagttcaa
      301 tttaaaggcg gtacaatatt agcaccatct tatatatgta atgattgatt tgcctcttta
      361 ttctactgtt gagattattg tttccgaaaa ttgattatta tcagcgcgaa aagaaatgct
      421 acagcaaata agaggacgat tgtcggcgct ggggctctgt tgagaaaata taataaccat
      481 acgcctgata ttgttattga tatcgttacg gcgataagta atgccttatg aaagcgatgc
      541 atgatgagga cagcaataga ccctggagct attaatagta tatagacaga ataattccga
      601 ctgcttttaa tgtggcaaaa atggtaggcg ataccatgca gagtaaaccg taatgtaata
      661 atgatatctg taatccgctg acttgtgcct gttgatagtc aagacaaaaa ttcctgaaaa
      721 aacgatacct ataatggtat ctttctttat aatgctatta tctttcagat agccagttgc
      781 aattgcacaa gacagtccta caataaatgc accaattccc agcggcatac ctataatcca
      841 gactagaact atgccaggaa acattgcgtg gctcattgca tctcctatta gcgcccagcc
      901 ttttaatacc agaaaaactg ataataatgc acaggggaac gatattataa tagcgattat
      961 caatgcataa ttcataaatt taaattgtaa tgattagagg ggaaaatatc agcacatttg
     1021 cgaaatgaat caaaaagccc gcagcaatgt gcgggcgtta gtgtcagcgc acaaccagca
     1081 cggagcactc tgcgtgacgc actacagctg cggcgttaga accgagcaga taagtggtga
     1141 tatccggtcg atgggaagca atgatgatca tatgagcggg gatcttcttc gccaattcca
     1201 gaatgcggtc tttgggcgag ccttcctcaa catggacatg cactctgtcg gttggcagtt
     1261 taaatttttt aatgatctct tccagttgcg atttggcttc cgctttcagg tcatccattg
     1321 ccggtaattc tgcggaatac gctaaaccca gagaggcata gtagggcagc gaaggtatta
     1381 ccgtcaggaa atgaacctct gcatcatcaa tctttgcctc tgcctcaacg tggctaatca
     1441 cgcgttgagt taattctgaa tcggaaatat cgatagggac aagaatcgtt ctgttcataa
     1501 aacctcctgt tttagtatcc gcataaagtg taacgccaga tgacactttt tgtgtaatga
     1561 cggagttcac atttttaatt tagatcaaag gaggaagaat aagcagaaaa agcccgccaa
     1621 aacagcgggc agggggattt agaactgata aaccagacct aaagcgacaa tatcatcggt
     1681 agagatgccg ttggcagcgt agaaactgtc atcttcatcc aacaggttga ttttatagtc
     1741 aacataggtg gacatgtttt tattgaaata gtaagtcgcg ccaacatcgg cgtatttaac
     1801 cagatcttta tcatcaacac ctgccggatt gtctgcacca ccggcagcgt gcaggtcacg
     1861 gcctttagac atcaggaaag agactgccgg acgcagacca aaatcaaact ggtactgtgc
     1921 agtgacttca aagttctggg ttttgtttgc aacagcataa tcgctgtcgc caaacggggt
     1981 catattacgc gtttctgaat acatggttgc caggtaaata ttgttagcat cgtattttag
     2041 cccagcagtc cacgcgtctg ctttatcacc acccgccgca gtatggttaa cctggtcatt
     2101 ggtgcggtca gaagaggtgt atgccgcacc agcgctaaag cccatgccta aatcatatgt
     2161 tgtggaaaga ccccagccgt caccgttttc atggcgaaca tcacgtccgt tgttggtgcc
     2221 ttcctgacca ttactggcac cttcgttgtt accttgatac tgcaccgcga agttcagacc
     2281 atttaccaga ccgaagaaat cagtattacg ataagtcgcg acgccattgg cacgaccagt
     2341 cataaagttg tctgcattgg tataagagtc accgccaaat tcaggtagca tatcagtcca
     2401 gccttcgatg tcgtacatta cgccataatt acgtccgtaa tcgaaagaac cgtaatctgc
     2461 aaatttcagt ccggcaaatg ccagacgggt ccatgactgg tttgttgaag attcggtgtt
     2521 gtttgcctga atattgtatt cccattgacc gtagccagtg agttgatcgt taatttgggt
     2581 ttcgccttta aaacccagac gagcatagct ctggtcgcca tctttcgctg aattatcaga
     2641 aaaataatgc aggccatcaa ctttgccata cagatccaat ttgttgccgt ctttattata
     2701 aacttcggct gcatgtgcag cacctgcagc gagcagggca ggaattaaga gtgccagtac
     2761 tttgcttttc attgaataaa tcctttagtt attttatttg cctttcatcc ccatttcggg
     2821 gagtgaaaac atcctaaagg agaagttcag ttagccgata aatat
//
LOCUS       DAHHTG010000259         2821 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.266, whole genome shotgun sequence.
ACCESSION   DAHHTG010000259 DAHHTG010000000
VERSION     DAHHTG010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2821)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2821)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2821
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.266"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            <1..815
                     /gene="waaO"
                     /locus_tag="NBI74_004582"
                     /gene_synonym="rfaI"
     CDS             <1..815
                     /gene="waaO"
                     /locus_tag="NBI74_004582"
                     /gene_synonym="rfaI"
                     /EC_number="2.4.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lipopolysaccharide
                     3-alpha-galactosyltransferase"
                     /protein_id="HCD1934788.1"
                     /translation="DNDRKYFDALALQYKTRIKIYLINGDRLRSLPSTKNWTHAIYFR
                     FVIADYFISKVAKVLYLDADIICQGTIEPLIKFSFPDDKVAMVVTEGQADWWEKRAHS
                     LGVAGISKGYFNSGFLLINTAKWAAQQVSARAIAMLNEPEVIKKITHPDQDVLNMLLA
                     DKLIFADIKYNTQFSLNYQLKESFINPVTNNTIFIHYIGPTKPWHDWAWDYPVSQAFM
                     EAKNASPWKNTALLKPNNSNQLRYSAKHMLKKHRYLKGFSNYLFYFIEKIKH"
     gene            855..1871
                     /gene="rfaJ"
                     /locus_tag="NBI74_004583"
     CDS             855..1871
                     /gene="rfaJ"
                     /locus_tag="NBI74_004583"
                     /EC_number="2.4.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide 1,2-glucosyltransferase RfaJ"
                     /protein_id="HCD1934789.1"
                     /translation="MDSFPAIEIDKVKAWDFRLANINTSECLNVAYGVDANYLDGVGV
                     SITSIVLNNRHINLDFYIIADVYNDAFFQKVAKLAEQYQLRITLYRINTDKLQCLPCT
                     QVWSRAMYFRLFAFQLLGLTLDRLLYLDADVVCKGDISQLLHLDLNGAVAAVVKDIDP
                     MQEKAASRLSDPELLGQYFNSGVVYLDLKKWANAKLTEKALSILMSKDNVYKYPDQDV
                     MNVLLKGMTIFLPRGYNTIYTIKSELKDKTHQNYKKLIIENTLLIHYTGATKPWHKWA
                     IYPSTKYYKIALDNSPWKDDSPRDAKSIIEFKKRYKHLLVQHHYISGIIAGVCYLCRK
                     YYPK"
     gene            1889..2587
                     /gene="rfaY"
                     /locus_tag="NBI74_004584"
     CDS             1889..2587
                     /gene="rfaY"
                     /locus_tag="NBI74_004584"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(II) kinase RfaY"
                     /protein_id="HCD1934790.1"
                     /translation="MIQKSKIKDLVVFTDEENSKYLNVLNDFLSYNINIIKVFRSIDD
                     TKVMLIDTDYGKLILKVFSPKVKRNERFFKSLLKGDYYERLFVQTQKVRNEGLNTLND
                     FYLLAERKTLRFVHTYIMIIEYIDGIELCDMPDIDDSLKNKIQQSINALHKHGMVSGD
                     PHRGNFIIKNGEVRIIDLSGKRASAQRKAKDRIDLERHYGIKNEIKDLGYYLLVYRKK
                     MRNFMRRLKGKPAR"
     gene            2657..>2821
                     /locus_tag="NBI74_004585"
     CDS             2657..>2821
                     /locus_tag="NBI74_004585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-D-manno-oct-2-ulosonate III transferase
                     WaaZ"
                     /protein_id="HCD1934791.1"
                     /translation="MKNIRYIDKKDVENLIENKTSDDVIIFLSGPTSQKTPLSVLRTK
                     DIIAVNGSVQY"
BASE COUNT          948 a          458 c          513 g          902 t
ORIGIN      
        1 gtgataatga tcgtaagtat tttgatgccc tggcgttaca gtataaaacc agaattaaaa
       61 tatatctgat taatggtgat cgactgcgct cattgcctag cacaaagaac tggactcatg
      121 caatatattt tcgttttgtt attgctgatt actttattag taaggtggct aaagtccttt
      181 atctggatgc agatatcatt tgtcagggga ctattgagcc actaattaaa ttctcatttc
      241 ctgacgataa agtcgcaatg gttgttacag aagggcaagc tgactggtgg gaaaaacgcg
      301 cccattcgtt aggtgttgcg ggaatttcta aaggttactt taactccggt tttttattga
      361 ttaatactgc caaatgggca gctcagcagg tttctgcacg agccattgca atgctaaatg
      421 agccagaagt aatcaaaaaa ataacacatc ctgatcagga tgtattaaat atgttgctgg
      481 cagataaact tattttcgct gatattaaat ataacaccca gtttagctta aattatcaac
      541 taaaagaaag ctttataaac ccagtaacca acaatactat ttttatccat tatatcgggc
      601 caaccaagcc ctggcatgat tgggcctggg attatcctgt atcacaagcc tttatggagg
      661 caaaaaatgc ttcgccatgg aagaatacag cgttgctgaa accgaacaat agcaatcaat
      721 taagatatag cgcaaaacat atgcttaaaa aacatagata tctaaaagga tttagcaact
      781 atctttttta ttttattgaa aagataaagc attaaaactg ggaaacaata aagtaatgat
      841 ataagggtag cattgtggac tcatttcctg ccatagagat agataaagtt aaagcctggg
      901 attttcggct ggctaatata aatacttctg aatgtttaaa tgttgcctat ggtgtcgatg
      961 ctaattatct tgatggtgta ggtgtttcca ttacatcaat tgtcctaaat aatcgacata
     1021 ttaatcttga tttttatatt attgctgatg tttataatga tgcttttttc caaaaagttg
     1081 caaagcttgc agagcaatac caattaagaa tcacattata tagaattaac actgataagc
     1141 tccagtgctt gccttgtaca caggtctggt caagagcaat gtacttccgt ttatttgcat
     1201 ttcaattatt gggtttaaca cttgatcgtt tgctttatct tgatgcagat gttgtttgta
     1261 aaggcgatat tagccaacta ttgcatctgg atttaaacgg cgcggttgct gctgttgtta
     1321 aagatattga cccaatgcag gaaaaggcgg catccaggtt gtctgatcca gaattactcg
     1381 ggcagtactt taattccggt gtcgtttatt tagatttaaa aaaatgggct aacgcaaaac
     1441 taacagaaaa agcgttgtct attcttatga gtaaagataa tgtgtataaa taccctgatc
     1501 aagatgttat gaatgtctta ctgaaaggga tgacaatatt tttgcctcgt ggatataata
     1561 caatttatac aattaaaagt gaattaaaag acaaaacaca tcaaaactat aaaaagctga
     1621 ttatagaaaa tactcttctt attcattata caggtgcaac taaaccgtgg cacaaatggg
     1681 caatttatcc atcgacaaaa tactataaaa tagctttaga caattccccc tggaaagatg
     1741 actctccacg agatgcgaaa tcaattattg aattcaaaaa aagatataaa catcttttag
     1801 tgcaacatca ctatatctca ggaattatag caggagtctg ttatctttgc cgaaaatatt
     1861 accctaaata acatttaact ggtttgttat gattcagaag agcaagatca aagacttggt
     1921 tgtttttacc gatgaagaga attcaaagta cctcaatgta ttaaatgact tcttgtctta
     1981 taatataaat atcatcaagg tttttcgttc tattgatgat acaaaagtta tgcttattga
     2041 taccgattac ggtaaattga ttcttaaggt tttttctccg aaagttaagc gtaacgaacg
     2101 tttctttaag tctctgttaa aaggtgatta ttacgaacgc ctttttgtgc aaactcaaaa
     2161 agtacgaaat gaagggttaa atacactcaa tgatttttat ttattggctg aacggaaaac
     2221 cttacgattt gtccatactt atatcatgat catcgagtat attgatggta tagagttatg
     2281 tgatatgccc gatattgatg attcgctaaa aaataaaatt caacaatcaa ttaatgcctt
     2341 gcataaacat ggcatggttt ctggcgaccc tcatcgtggt aacttcatta taaaaaatgg
     2401 tgaggtccga attattgatc tctctggaaa acgtgcttca gcgcagcgta aagcgaaaga
     2461 tcgcattgac ttagagcgtc attacggtat taaaaatgag attaaagatc taggctatta
     2521 tcttttagta tatcgtaaaa aaatgcgcaa ttttatgcgg cgtttgaaag ggaaaccagc
     2581 tcgctaaaaa aatcccacaa ttacatgtct tcaccaggac agttttaagc ggagttattt
     2641 atttcacagg tttatcatga agaatattag atatattgac aaaaaagatg ttgaaaacct
     2701 gattgaaaat aaaacatctg atgatgttat tatttttctt tcgggcccta catcgcaaaa
     2761 aacacctttg tcagtattac gaacaaagga tattattgct gtcaatggtt ctgtgcaata
     2821 t
//
LOCUS       DAHHTG010000260         2755 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.267, whole genome shotgun sequence.
ACCESSION   DAHHTG010000260 DAHHTG010000000
VERSION     DAHHTG010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2755)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2755)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2755
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.267"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            79..2358
                     /gene="ydeP"
                     /locus_tag="NBI74_004586"
     CDS             79..2358
                     /gene="ydeP"
                     /locus_tag="NBI74_004586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance putative oxidoreductase YdeP"
                     /protein_id="HCD1934792.1"
                     /translation="MKKKIESYQGAAGGWGAVKSVANAVRKQMDIRQDVIAMFDMNKP
                     EGFDCPGCAWPDPKHSASFDICENGAKAIAWEVTDKQVNASFFAENTVQSLLTWGDHE
                     LEAAGRLTQPLKYDDVSDCYKPLSWQQAFDEIGARLQSYSDPNQVEFYTSGRTSNEAA
                     FLYQLFAREYGSNNFPDCSNMCHEPTSVGLAASIGVGKGTVLLEDFEKCDLVICIGHN
                     PGTNHPRMLTSLRALVKRGAKMIAINPLQERGLERFTAPQNPFEMLTNSETQLASAYY
                     NVRIGGDMALLKGMMRLLIERDDAASAAGRPSLLDDEFIQTHTVGFDELRRDVLNSEW
                     KDIERISGLSQTQIAELADAYAAAERTIICYGMGITQHEHGTQNVQQLVNLLLMKGNI
                     GKPGAGICPLRGHSNVQGDRTVGITEKPSAEFLARLGERYGFTPPHAPGHAAIASMQA
                     ICTGQARALICMGGNFALAMPDREASAVPLTQLDLAVHVATKLNRSHLLTARHSYILP
                     VLGRSEIDMQKSGAQAVTVEDSMSMVHASRGVLKPAGVMLKSECAVVAGIAQAALPQS
                     VVAWEYLVEDYDRIRNDIEAVLPEFADYNQRIRHPGGFHLINAAAERRWMTPSGKANF
                     ITSKGLLEDPSSAFNSKLVMATVRSHDQYNTTIYGMDDRYRGVFGQRDVVFMSAKQAK
                     ICRVKNGERVNLIALTPDGKRSSRRMDRLKVVIYPMADRSLVTYFPESNHMLTLDNHD
                     PLSGIPGYKSIPVELEPSN"
     gene            2606..>2755
                     /gene="safA"
                     /locus_tag="NBI74_004587"
     CDS             2606..>2755
                     /gene="safA"
                     /locus_tag="NBI74_004587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system connector SafA"
                     /protein_id="HCD1934793.1"
                     /translation="MHATTVKNKITQRDNYKEIMSVIVVVLLLTLTLIAIFSAIDQLG
                     ISEMGR"
BASE COUNT          740 a          644 c          674 g          697 t
ORIGIN      
        1 gacgataaac cctatgtata tattgtgcat catcgctatt acaaatccta ataattcatt
       61 tccacacagg ataagcagat gaagaaaaaa attgaatcct accagggagc tgccggtggt
      121 tggggtgctg ttaaatccgt agcgaatgca gtacgtaagc agatggatat acgccaggat
      181 gttattgcca tgtttgacat gaataagcca gagggctttg actgtccggg atgtgcatgg
      241 ccagatccta agcacagtgc gtcattcgac atttgtgaaa acggcgcaaa agcaatcgcc
      301 tgggaagtca cggataagca ggtaaatgcc tctttctttg ctgagaatac ggttcaatca
      361 ttacttacct ggggagatca cgagcttgag gctgcgggac gtctcactca gcctttgaaa
      421 tatgatgacg tcagcgactg ttacaagcca ttaagctggc aacaagcttt cgacgaaatt
      481 ggcgctcgcc ttcaaagcta tagtgatccc aatcaggttg aattctatac ttcggggcgc
      541 acttccaatg aagctgcctt tctttatcag ctttttgccc gtgaatacgg gagcaataac
      601 tttcccgact gctccaacat gtgccatgaa ccgaccagtg tgggattggc agcgagtatc
      661 ggtgtgggta aagggaccgt gttgctggaa gactttgaga aatgcgattt agtcatttgc
      721 attgggcata accctggtac aaaccaccct cgcatgctga cttcgttgcg cgctttagtg
      781 aaacggggag cgaaaatgat cgctatcaac cctctacagg aacgtggcct ggagcgattt
      841 accgcaccgc aaaacccgtt tgaaatgctg acgaactctg agactcagtt agccagtgcc
      901 tactataacg tgcgcattgg tggcgatatg gcgttgctca aggggatgat gcgcctgtta
      961 attgagcgcg atgatgctgc aagcgccgca ggtcggccct cattgctgga tgacgaattt
     1021 attcaaacgc ataccgtcgg ctttgacgag ctacgccgtg acgtgctcaa ttccgagtgg
     1081 aaagatatcg aacgtatttc tggactaagt cagacacaaa tcgccgaact tgctgatgca
     1141 tatgccgctg ccgaacgaac cattatctgt tacggaatgg ggatcactca gcacgaacat
     1201 ggtacccaga acgtacagca actggtcaat ctgctgttga tgaaaggtaa cattggcaag
     1261 cctggtgcgg gtatctgccc actacgaggt cactctaatg tacagggcga ccgaaccgtc
     1321 ggtatcaccg agaaaccgtc tgcagagttt ctggctcgcc tgggtgagcg ctatggcttc
     1381 accccacctc atgcacctgg acatgctgca atagccagca tgcaagctat atgtacgggg
     1441 caagctcgag cattgatctg catggggggc aactttgcac tggcaatgcc agatcgggaa
     1501 gcgagcgctg taccgttaac gcaattagat ctggcggtac acgtagccac taagcttaac
     1561 cgctctcatc tgctgaccgc acggcatagc tatattctgc cggttctggg acgtagcgag
     1621 attgacatgc aaaaaagcgg tgcgcaggcg gtaaccgttg aggattcaat gtcgatggtt
     1681 catgcctcgc gtggtgtgtt aaaacccgcc ggtgtaatgc tgaaatcaga gtgtgcagtg
     1741 gtcgcgggaa tcgcgcaggc agcactaccc cagagcgtgg tagcctggga gtatctggtg
     1801 gaagattatg atcgcattcg caatgacatt gaagctgtgc tgccagagtt tgccgactat
     1861 aaccagcgca tccgtcatcc tggtggtttt cacctgataa acgcagctgc tgaaaggcgc
     1921 tggatgacgc cgtcaggtaa ggctaatttc attaccagca aagggctgtt agaagatcct
     1981 tcttcagcgt ttaacagtaa gctggtcatg gcgacagtac gcagccacga tcagtacaac
     2041 acgacgattt atggtatgga tgatcgctat cgaggtgtat tcggtcaacg agatgtggtc
     2101 tttatgagtg ctaaacaagc taaaatttgc cgtgtaaaaa acggcgaaag agttaatctt
     2161 attgcactca cgccagacgg taaacgtagc tcacgccgca tggatagatt aaaagtggtc
     2221 atttacccta tggctgaccg ctcactggtg acctatttcc cagaatcgaa tcacatgcta
     2281 acacttgata accacgatcc attaagtggc attcctggct ataaaagtat tccggttgaa
     2341 ttagaaccat caaattaatt ctcctcctca tttcttctgc tgtcatccgc acagcagaag
     2401 aattcctcat tgactattat cgcgcaattt gctcacatgg attaaattaa actacacact
     2461 ataagatata aacctctgcc tacagttgta agaaactccg ctcagtactg aagtgccagt
     2521 cctatttcct cttttatcca gcctgttata ttaatcatac tgattaacga tttttaacgt
     2581 tatccgctaa ataaacacat ttgaaatgca tgcgaccaca gtgaaaaaca aaatcacgca
     2641 aagagacaac tataaagaaa tcatgtctgt aattgtggtt gtcttgttac tgacacttac
     2701 gttgatagcc attttttcgg caattgatca gctgggcatt tcagaaatgg gtcgc
//
LOCUS       DAHHTG010000261         2750 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.268, whole genome shotgun sequence.
ACCESSION   DAHHTG010000261 DAHHTG010000000
VERSION     DAHHTG010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2750)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2750)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2750
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.268"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            complement(<1..2691)
                     /gene="chiA"
                     /locus_tag="NBI74_004588"
     CDS             complement(<1..2691)
                     /gene="chiA"
                     /locus_tag="NBI74_004588"
                     /EC_number="3.2.1.14"
                     /EC_number="3.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chitinase/lysozyme"
                     /protein_id="HCD1934794.1"
                     /translation="MKLNIFTKSMIGMGLVCSSLPALAMEAWNNQQGGNKYQVIFDGK
                     IYENAWWVSSTNCPGKAKANDATNPWRLKRTATAAEISQFGNTLSCEKSGSSSSSTSN
                     TPASNTPANGGSATPAQGTVPSNSSVVAWNKQQGGQTWYVVFNGAVYKNAWWVASSNC
                     PGDAKGNDASNPWRYVRAATATEISETSNPQSCTSAPQPAPDVKPAPDVKPAPDVQPA
                     PADKSNDNYAVVAWKGQEGSSTWYVIYNGSIYKNAWWVGAANCPGDAKENDASNPWRY
                     VRAATATEITQYGNPGSCSVKPDNNGGAVTPVDPTPETPETPVTPTPDNNEPSTPADS
                     SNDYSLQAWSGQEGSEIYHVIFNGNVYQNAWWVGSEDCPRGTSVENSNNPWRLVRTAT
                     AAEMSQYGNPTTCEIDNGGVIIVDGFQASKAYSANSIVDYNDAHYKTSVDQDAWGFVP
                     GGDNPWKKYEPAKAWSASTVYVKGDRVVVDGQAYEALFWTQSDNPALVANQNATGSNS
                     RPWKPLGKTQSYSNEELNNAPQFNPETLYASDTLIRFNGENYISQSKVQKVSPSDSNP
                     WRVFVDWTGTKERVGTPKKAWPKHVYAPYVDFTLNTIPDLAALAKNHNVNHFTLAFVV
                     SKDANTCLPTWGTAYGMQNYAQYSKIKALREAGGDVMLSIGGANNAPLAASCKNVDDL
                     MQHYYDIVDNLNLRVLDFDIEGTWVADQASIERRNLAVKKVQDKWKSEGKDIAIWYTL
                     PILPTGLTPEGMNVLSDAKAKGVELAGVNVMTMDYGNAICQSANTEGQNIHGKCATSA
                     IANLHAQLKGLYGNKSDAEIDAMMGTTPMVGVNDVQGEVFYLSDARLVMQDAQKRNLG
                     MVGIWSIARDLPGGTNLSPEFHGLTKEQAPKYAFSEIFAPF"
BASE COUNT          650 a          725 c          644 g          731 t
ORIGIN      
        1 aaacggcgcg aagatttcgc taaatgcgta cttcggagcc tgttctttag tcagaccgtg
       61 gaattccgga gacaggttag tgccgcccgg caggtcgcgc gcgattgacc agataccaac
      121 cataccgaga ttacgcttct gcgcatcctg catgaccaga cgagcatcag agagatagaa
      181 cacctcaccc tgaacgtcgt tcacgccaac catcggcgtg gtacccatca tggcgtcaat
      241 ttctgcatca ctcttattgc catagaggcc tttcagctgc gcatgcaggt tggcaatcgc
      301 agacgttgca cacttaccgt ggatgttttg gccttcggtg tttgcagact gacaaatagc
      361 gttaccgtag tccattgtca tcacgttcac acccgccagc tcaacacctt tcgctttggc
      421 atcgctcagg acattcatcc cttccggcgt caggcctgtc ggcagaattg gcaaggtata
      481 ccagatagca atatctttgc cttctgactt ccatttatcc tgcactttct tcacagcaag
      541 gttacgacgt tcaatagatg cctgatccgc aacccaggtg ccttcgatat cgaagtccag
      601 gactctgagg ttcaggttat caacgatgtc ataataatgc tgcattagat cgtcaacgtt
      661 cttacaggaa gctgccagcg gggcattatt agcaccaccg atagacagca tcacatcacc
      721 gccagcctca cgcagcgctt tgattttgct gtactgagcg taattctgca taccgtaagc
      781 ggtcccccat gtcggcagac aggtgttcgc atctttactc accacaaacg ccagcgtgaa
      841 gtggttgacg ttatgattct tagccagcgc agccagatcc gggatcgtat tcagcgtaaa
      901 gtcgacatac ggtgcataaa cgtgtttcgg ccacgctttc ttcggcgtac ctacgcgctc
      961 tttggttccg gtccagtcaa caaagacacg ccacgggttg ctgtcagaag gagaaacttt
     1021 ctgcacttta ctctgagaaa tgtagttctc accgttaaag cgaatcagcg tatcactggc
     1081 ataaagcgtt tctggattaa actgcggcgc attattcagc tcttcgttgc tatagctctg
     1141 agtcttacct aacggcttcc acgggcgact attgctaccg gtggcgtttt ggttcgcaac
     1201 cagagcaggg ttgtcacttt gcgtccagaa cagcgcttca taagcctgcc catcaacaac
     1261 aacgcgatca cctttcacgt acacggtgga agcggaccat gctttcgccg gttcgtattt
     1321 cttccacggg ttatcgccgc cggggacaaa gccccatgcg tcttgatcga cagaggtttt
     1381 ataatgtgca tcgttataat ctacgatgct gttcgcgcta tacgctttgc tggcctggaa
     1441 accatccaca ataatgacgc cgccattatc aatttcacag gtagtcgggt taccgtactg
     1501 actcatttcc gcagcggtag ctgtacgcac gagacgccat gggttattgg agttttctac
     1561 actggtgcca cgcgggcaat cttcagaacc aacccaccag gcattctgat aaacattacc
     1621 attgaagata acatggtaaa tttcgctgcc ttcctggcca ctccacgcct gcagtgagta
     1681 atcgttactg ctatccgctg gtgttgatgg ctcgttgtta tccggagttg gagtaactgg
     1741 agtttccggt gtttccggag ttggatcaac cggagtcaca gcaccgccat tattatccgg
     1801 cttaacggaa caggagcctg ggttaccata ctgagtgatt tctgttgccg ttgccgcgcg
     1861 aacataacgc catgggttgc tggcatcgtt ttctttcgca tcgcctgggc aatttgccgc
     1921 gcctacccac caggcgttct tataaatgct gccgttgtag ataacgtacc atgtagaaga
     1981 accttcctga cctttccagg ctacaacagc atagttgtcg tttgacttat cagctggggc
     2041 tggctgaaca tcaggggccg gtttaacgtc cggtgccggt ttcacatccg gtgcaggctg
     2101 tggtgctgaa gtacaagact gtggattact ggtttctgag atttccgttg ccgtagcggc
     2161 acgaacataa cgccatgggt tgctggcatc attgcctttc gcatcacccg ggcagttaga
     2221 agaggctacc caccaggcat ttttatatac cgcaccatta aagacgacat accaggtttg
     2281 accgccctgc tgtttattcc aggcaactac agaagaatta gacggaacag tgccctgtgc
     2341 tggtgtagcc gaaccgccat tagcgggcgt attagatgct ggcgtatttg aagttgaaga
     2401 agatgagctt ccgctctttt cgcaggaaag tgtattgcca aactgactaa tttcagcagc
     2461 tgttgcggta cgctttaaac gccacgggtt agttgcatca tttgctttcg cttttcccgg
     2521 gcaatttgta gaagaaaccc accaggcatt ttcataaatt ttgccatcga aaataacctg
     2581 atatttatta ccaccttgtt gattattcca tgcttccatt gccaatgctg gcagagagga
     2641 acacaccagc cccataccaa tcatagattt agtaaatata tttaatttca ttataatccc
     2701 ttgtaacgta aaaactgcaa aacaaaagtc ctacgcagga attatttacg
//
LOCUS       DAHHTG010000262         2749 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.269, whole genome shotgun sequence.
ACCESSION   DAHHTG010000262 DAHHTG010000000
VERSION     DAHHTG010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2749)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2749)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2749
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.269"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            complement(<1..600)
                     /locus_tag="NBI74_004589"
     CDS             complement(<1..600)
                     /locus_tag="NBI74_004589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HCD1934795.1"
                     /translation="MGLFTTRQLLGYTEQKVKFRALFLELFFRRTVNFHTEEVMLDKI
                     TGKTPVAAYVSPIVEGKVLRHRGGETRVLRPGYVKPKHEFNYQQAVERLPGEDPSQLN
                     DPAYRRLRIITDNLKQEEHAIVQVEEMQAVNAVLYGKYTMEGDQFEKIEVDFGRSTKN
                     NITQGSGKEWSKQDRDTFDPTHDIDLYCDQASGLVNIAIM"
     gene            complement(658..1005)
                     /locus_tag="NBI74_004590"
     CDS             complement(658..1005)
                     /locus_tag="NBI74_004590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head decoration protein"
                     /protein_id="HCD1934796.1"
                     /translation="MVTKTITEQRAEVRIFAGNDPAHTATGSSGISSATPALTPLMLD
                     EATGKLVVWDGQKAGSAVGILVLPLEGTETALTYYKSGTFATEAIHWPESVDEHKKAN
                     AFAGTALSHAALP"
     gene            complement(1042..2490)
                     /locus_tag="NBI74_004591"
     CDS             complement(1042..2490)
                     /locus_tag="NBI74_004591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HCD1934797.1"
                     /translation="MRRNLSHIIAAAFNEPLLLEPAYARVFFCALGREIGAASLSVPQ
                     QQVQLDAPGMLAETDEYMAGGKRPARVYRVVNGIAVLPVTGTLVHRLGGMRPFSGMTG
                     YDGIVACLQQAMADSQVRGVLLDIDSPGGQAAGAFDCADMIYRLRQQKPVWALCNDTA
                     CSAAMLLASACSRRLVTQTSRIGSIGVMMSHVSYAGHLAQAGVDITLIYAGAHKVDGN
                     QFEALPAEVRQDMQQRIDAAHRMFAEKVAMYTGLSVDAVTGTEAAVFEGQSGIEAGLA
                     DELINASDAISVMATALNSNVRGGTMPQLTATEAAVQENQRVMGILTCQEAKGREQLA
                     TMLAGQQGMSVEQARAILAAAAPQQPVASAQSEADRIMACEEANGREQLAAAPLADAG
                     PSLRDQIMALDEAKGAEAQAEKLAACPGMTVENARAVLAAGSGKAEPVSASTTALFEH
                     FMANHSPAAVRGGVSQTSADGDADVKMLMAMP"
     gene            complement(2480..>2749)
                     /locus_tag="NBI74_004592"
     CDS             complement(2480..>2749)
                     /locus_tag="NBI74_004592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HCD1934798.1"
                     /translation="EWIGAGRMAIDGLKEVQESVMRIEAGLSTYEKELALMGEDYQDI
                     FRQQVRESAEREKAGLSRPVWIAQAYQQQIAESRRPEEETTPRET"
BASE COUNT          553 a          869 c          684 g          643 t
ORIGIN      
        1 cataatggca atattcacaa gaccgctggc ctggtcgcag tagaggtcaa tatcatgtgt
       61 aggatcgaac gtgtcacgat cctgttttga ccactcctta ccactaccct gagtgatgtt
      121 attcttcgtc gacctgccaa aatcgacctc aattttctcg aactggtctc cttccatggt
      181 gtatttgcca tacaacacag catttaccgc ctgcatttct tccacctgga caatggcgtg
      241 ctcttcctgt ttgaggttat cggtaatgat acgcagacgg cggtaagccg gatcattcag
      301 ttgagatgga tcttcaccag gaaggcgctc aaccgcctgc tggtaattaa attcgtgttt
      361 cggcttgacg tagcccggac gcaacacgcg ggtttcacca ccacgatggc gcagcacttt
      421 tccttcaacg atcggggaga cataggccgc caccggcgtt tttccggtaa ttttgtccag
      481 catcacctct tcggtgtgga aattcaccgt acggcggaaa aacagctcca gaaatagcgc
      541 acggaattta actttttgtt cggtataacc gagtaactgg cgggtcgtaa acaatcccat
      601 aaatcagttc ctttcattca gaaatcagtc aggccaccat ggtggcctga taacgtgtta
      661 cggcagagcc gcgtgactca gggctgtgcc ggcaaaggca tttgcctttt tgtgttcatc
      721 cacactttca ggccagtgga ttgcctccgt cgcaaaggtt cccgacttgt aatacgtcag
      781 cgctgtctct gtgccttcaa gcggcagtac cagtatgcca accgcactac cggctttctg
      841 tccatcccag accaccagtt tcccggtggc ttcatccagc atcaggggcg tcagagccgg
      901 tgttgcggaa gaaatcccgc tgctgcctgt ggcggtatga gccggatcat taccggcaaa
      961 aatacgtact tccgcacgct gttcagtgat ggttttcgtc accattttgt taaaacctca
     1021 tattgatggt cagcactgac ttcatggcat ggccatgagc attttcacgt ccgcatcacc
     1081 gtctgctgac gtctgtgaca cgccaccccg caccgctgcc ggtgaatgat tcgccatgaa
     1141 atgttcaaac agggcggttg tggatgcaga gaccggttcg gccttacctg atcccgcagc
     1201 cagcacagcc cgggcgttct ccacggtcat tcccgggcag gccgccagtt tttcagcctg
     1261 cgcttctgcc ccttttgcct catccagggc catgatctga tcacgaagtg agggcccggc
     1321 atccgccagt ggtgcagccg ccagttgttc acgaccgttc gcttcttcac acgccataat
     1381 gcgatcggct tcactctgcg cggatgccac cggctgctgc ggtgccgccg cggccagaat
     1441 cgcccgggcc tgttcaacgc tcatgccctg ttgccctgcc agcatcgtgg caagctgttc
     1501 acgtcctttc gcttcctggc atgtcaggat ccccatcact cgctggttct cctgcacggc
     1561 ggcttccgtt gcagttaatt gcggcatagt gcctcctctg acattactgt tcagcgccgt
     1621 ggccatcaca ctgatggcat ccgacgcatt gattaattca tccgccagcc cggcctcaat
     1681 gccggactga ccttcaaaaa cggcggcctc tgttcccgtg accgcatcca cagacaaccc
     1741 ggtatacatc gccacttttt cggcaaacat ccggtgcgcc gcatcaatcc gctgctgcat
     1801 gtcctggcga acctctgccg gcaacgcttc aaactgattg ccatccacct tgtgcgcccc
     1861 ggcataaatc agcgtgatat ccacaccggc ctgcgccaga tgaccggcat agctgacatg
     1921 gctcatcatc acgccaatgg agccgatacg ggatgtctgg gtaaccagcc gtcgggagca
     1981 ggccgacgcc agcagcatgg ctgcagaaca ggcagtgtca ttgcacagtg cccagaccgg
     2041 cttctgctga cggaggcggt aaatcatgtc agcgcagtca aacgcgccgg cggcctgccc
     2101 gcccggactg tcaatgtcca gcagtacgcc ccgcacctgg ctatccgcca ttgcctgctg
     2161 aagacaggcg acaatgccgt catagcctgt cattccggaa aatggccgca taccgcccag
     2221 ccggtgcacc agcgtgccgg tcaccggcag tacagcaata ccgttcacca cccggtaaac
     2281 acgggccggt cgtttacctc cggccatgta ctcgtccgtt tcagccagca ttccgggagc
     2341 atcaagctgt acctgttgtt gtggtaccga aagacttgct gcccctatct cgcgcccgag
     2401 cgcgcaaaag aaaacccgcg cataggcggg ctccagaagc agcggttcat tgaatgctgc
     2461 ggcaataatg tgtgaaagat tacgtctcac gtggtgttgt ctcctcttcc ggcctgcgac
     2521 tctccgctat ctgctgctga tacgcctgcg ctatccacac cggacgtgag agtccggctt
     2581 tttcccgctc tgcagattcc ctgacctgct ggcggaaaat gtcctgataa tcctcgccca
     2641 tcagcgccag ctctttctca tacgtgctca gtccggcctc aatgcgcatc actgattcct
     2701 gaacctcctt gagcccgtca atggccattc ttccggctcc aatccactc
//
LOCUS       DAHHTG010000263         2653 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.270, whole genome shotgun sequence.
ACCESSION   DAHHTG010000263 DAHHTG010000000
VERSION     DAHHTG010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2653)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2653)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2653
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.270"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            516..659
                     /locus_tag="NBI74_004593"
     CDS             516..659
                     /locus_tag="NBI74_004593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YobF family protein"
                     /protein_id="HCD1934799.1"
                     /translation="MCGIFSKEVLSKHVDVEYRFSAEPYIGASCSNVSVLSMLCLRAK
                     KTI"
     gene            672..881
                     /gene="cspE"
                     /locus_tag="NBI74_004594"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             672..881
                     /gene="cspE"
                     /locus_tag="NBI74_004594"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HCD1934800.1"
                     /translation="MAKIKGQVKWFNESKGFGFITPADGSKDVFVHFSAIQGNGFKTL
                     AEGQNVEFEIQDGQKGPAAVNVTAI"
     gene            1047..1856
                     /gene="rlmA"
                     /locus_tag="NBI74_004595"
     CDS             1047..1856
                     /gene="rlmA"
                     /locus_tag="NBI74_004595"
                     /EC_number="2.1.1.187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(745)-N(1))-methyltransferase"
                     /protein_id="HCD1934801.1"
                     /translation="MSFSCPLCHQPLLREKNSYICPQRHQFDMAKEGYVNLLPVQHKR
                     SRDPGDSAEMMQARRAFLDAGHYQPLRDAIVAQLRERLDEKATAVLDIGCGEGYYTHA
                     FADALPEITTFGLDVSKVAIKAAAKRYPQVTFCVASSHRLPFSDTSMDAIIRIYAPCK
                     AEELVRVVKPGGWVITATPGPRHLMELKGLIYNEVHLHAPHAEQLKGFTLQQSDELCY
                     LMRLRGDEAVALLQMTPFAWRAKPEVWQALAAKEVFDCQTDFNIHLWQRSY"
     gene            complement(1853..2419)
                     /gene="mntP"
                     /locus_tag="NBI74_004596"
     CDS             complement(1853..2419)
                     /gene="mntP"
                     /locus_tag="NBI74_004596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP"
                     /protein_id="HCD1934802.1"
                     /translation="MNITATVLLAFGMSMDAFAASIGKGATLHKPKFSEALRTGLIFG
                     AVETLTPLIGWGMGMLASRFVLEWNHWIAFVLLIFLGGRMIIEGFRGADDEDEEPRRR
                     HGFWLLVTTAIATSLDAMAVGVGLAFLQVNIIATALAIGCATLIMSTLGMMVGRFIGS
                     IIGKKAEILGGLVLIGIGVQILWTHFHG"
BASE COUNT          703 a          605 c          629 g          716 t
ORIGIN      
        1 agatatttac cccattaatt tattaggatg tttacatcgg atttgtgatt aagtgtggta
       61 ttatttatta gcgaaacgtt tctctcttga tttttttgct aattcatcaa tttttcttat
      121 tttaaattta caataccttt ggagattgac ttctctttgg ggtaattgat acccgttaac
      181 tgactgtttt atgagaaaaa gtgatataac tttttattca ttgcatagta ggaaatgtga
      241 tattgcacgc actatgtaat aacttctccc actggcctgg aacaactgaa cttattgaac
      301 tatgttagaa aatacgtcag tttaagtatc tgcctgaact ggcaaggtta agcacaatga
      361 tatatcggcg cgtattccgt tgcataagtg tgcaaaaaaa gtggaagacg tatcgagatt
      421 tgtgcgtctg atcgagacat gtttaaaaat ggcttgccat aattaacgtt gtatgtgata
      481 acagatttcg ggttaaacga ggtacagttc tgtttatgtg tggcattttc agtaaagaag
      541 tcctgagtaa acacgttgac gttgaatacc gcttctctgc cgagccttat attggtgcct
      601 catgcagtaa tgtgtcagtt ttatctatgt tatgcctgcg ggcgaagaaa acaatctaag
      661 gaatttttca aatggcaaag attaaaggtc aggttaagtg gttcaacgag tctaaaggtt
      721 ttggcttcat tactccggct gatggcagca aagatgtgtt cgtacacttc tccgctatcc
      781 agggtaatgg tttcaaaact ctggctgaag gtcagaacgt tgagttcgaa attcaggacg
      841 gtcagaaagg tccggcagct gttaacgtaa cagctatctg atcgaatcca ctgatctgaa
      901 gtgtgaatac gcttcaatct cgctataaag cctcgtcgaa tgcgaggctt tttactttgt
      961 tttatcttcg ctccagtcgt tcggatattt gcccgccgca tgattcgcgt tacacttgcg
     1021 gcctttagta tcctgccgga gttgtcatgt cgttttcctg tccactttgc catcagcctc
     1081 ttttgcgtga aaaaaacagc tatatctgtc cccagcgaca tcagtttgat atggcgaaag
     1141 aagggtatgt caatctgctg cccgttcagc ataaacggtc tcgtgatccg ggcgacagcg
     1201 cggaaatgat gcaagcacgc cgcgcattct tagatgccgg acattatcag ccgctgcgtg
     1261 atgcaattgt cgcccaactg cgggaacggc ttgatgaaaa ggccacggcg gtgctggata
     1321 ttggctgtgg tgaagggtat tacacacacg catttgctga tgcgttgccc gaaatcacca
     1381 cgtttggcct ggatgtctcg aaggtagcga taaaagcggc ggcgaaacgc tatccgcagg
     1441 tcactttttg tgtcgcttcc agccaccgtt tgccgttttc cgataccagt atggacgcca
     1501 taatacgtat ttacgcgccg tgtaaagcag aagaattagt gcgagtagtg aagcccggcg
     1561 gttgggtcat tactgccacg ccgggaccga gacacttaat ggagctgaag gggctgattt
     1621 acaatgaagt acatcttcat gcacctcatg cagaacaact gaaaggtttt acattacagc
     1681 agagtgatga gttgtgttat ctgatgcgtc ttcgcggtga tgaagccgtc gcattattgc
     1741 agatgacgcc gtttgcctgg cgtgcgaagc ctgaagtctg gcaagcactg gcagcaaaag
     1801 aagtgttcga ctgccagacg gactttaata ttcacctctg gcagcgttct tattaaccgt
     1861 ggaagtgcgt ccagaggatc tggacgccga tgccgatcag taccagcccg ccgagaattt
     1921 ccgctttttt cccaataatt gagccgataa agcgaccaac catcatccct aatgtcgaca
     1981 taatcaaggt tgcacaacca atggccaatg cggtcgcgat aatgttaacc tgcaggaaag
     2041 caagaccaac acccacagcc atggcatcca ggctggtggc aatcgcagtg gttaccagta
     2101 accagaaacc gtgtcgacgg cgcggttctt catcttcatc atctgcgcca cgaaaaccct
     2161 caataatcat tcgcccgccg agaaatatca gcagcacaaa cgcaatccag tggttccatt
     2221 caaggacaaa ccggctggct aacatgccca ttccccagcc gatcagcggc gtcagggttt
     2281 cgacggcacc aaaaataagg ccggttcgca atgcttcaga aaatttcggt ttatggaggg
     2341 tggcaccttt accgattgat gcagcaaagg catccatcga cataccaaac gcaagaagaa
     2401 cagtagcagt gatattcatg acaacgtcct gaccggggta tccataaaac acatcatcgc
     2461 ccccagcaaa catgcataca tgaacaacaa gccgcgatgg ttttcgcgtt catgcaggca
     2521 tagcgattga tgtgcatatg gtctcgcctg actgaaagtg ttcagtccgt acgtgccacg
     2581 ttttgcaacg agtatgttga cacgtacatt tccggaatct ggaaatcagc tactccccaa
     2641 atgagggcgc aac
//
LOCUS       DAHHTG010000264         2546 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.271, whole genome shotgun sequence.
ACCESSION   DAHHTG010000264 DAHHTG010000000
VERSION     DAHHTG010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2546)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2546)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2546
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.271"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            complement(99..953)
                     /gene="rclR"
                     /locus_tag="NBI74_004597"
     CDS             complement(99..953)
                     /gene="rclR"
                     /locus_tag="NBI74_004597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RclR"
                     /protein_id="HCD1934803.1"
                     /translation="MDALSRLLMLNAPQGTIDKNCVLGSDWQLPHGAGELSVIRWHAL
                     TQGAAKLEMPTGEIFTLRPGNVVLLPQNSAHRLSHVDNESTCIVCGTLRLQHSARYFL
                     TSLPETLFVAPVNHSVEYNWLREAIPFLQQESRSAMPGVDALCSQICATFFTLAVREW
                     IAQVNTEKNILSLLLHPRLGAVIQQMMEMPGHAWTVESLASIAHMSRASFAQLFRDVS
                     GTTPLAVLTKLRLQIAAQMFSREMLPVVVIAESVGYASESSFHKAFVREFGCTPGEYR
                     ERVRQLAP"
     gene            1180..2505
                     /locus_tag="NBI74_004598"
     CDS             1180..2505
                     /locus_tag="NBI74_004598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridine nucleotide-disulfide oxidoreductase"
                     /protein_id="HCD1934804.1"
                     /translation="MNKYQAVIIGFGKAGKTLAVTLAKAGWRVALIEQSNAMYGGTCI
                     NIGCIPTKTLVHDAQQHTDFVRAIQRKNEVVNFLRNKNFHNLADMPNIDVIDGQAEFI
                     NNHSLRVHRPEGNLEIHGEKIFINTGAQTVVPPIPGITTTPGVYDSTGLLNLKELPGH
                     LGILGGGYIGVEFASMFANFGSKVTILEAASLFLPREDRDIADNIATILRDQGVDIIL
                     NAHVERISHHENQVQVHSEHAQLAVDALLIASGRQPATASLHPENAGIAVNERGAIVV
                     DKQLHTTADNIWAMGDVTGGLQFTYISLDDYRIVRDELLGEGRRSTDDRKNVPYSVFM
                     TPPLSRVGMTEEQARESGADIQVVTLPVAAIPRARVMNDTRGVLKAIVDNKTQRILGA
                     SLLCVDSHEMINIVKMVMDAGLPYSILRDQIFTHPSMSESLNDLFSLVK"
BASE COUNT          724 a          572 c          591 g          659 t
ORIGIN      
        1 atataaaacc caaaaccacc gttagaaata gaaatagttg aaaggctcac aactcccgga
       61 atgcggatta tctttgccct gatttctggc ggttttattc agggtgcaag ctgtctcacc
      121 ctttcccgat attctcccgg agtacaacca aactcgcgga caaacgcctt gtgaaaagat
      181 gattcactgg catagcctac tgactcagcg atcaccacaa cagggagcat ttcccgggaa
      241 aacatctggg ccgctatttg tagacgcaac tttgttaata cagccagcgg cgtggttccg
      301 gaaacatcac ggaaaagctg ggcaaaactt gcccgggaca tgtgggcgat gctggccagc
      361 gattcgacgg tccaggcgtg tccaggcatt tccatcattt gctgtattac cgcaccaaga
      421 cgtggatgca gaagcaaact gagaatgttt ttctctgtat taacctgtgc aatccactca
      481 cgcaccgcga gggtaaagaa tgtagcgcag atctggctac acagagcatc cactcccggc
      541 attgccgatc tggattcctg ttgtaaaaac gggatcgcct ccctcagcca gttatattcc
      601 acgctatggt tcactggtgc tacaaacagc gtttccggca aagacgttaa aaaataacgc
      661 gccgaatgtt gcagccgaag agttccacag acaatacagg tcgattcatt atcgacatga
      721 ctcaggcgat gagctgaatt ttgtggtagc aggaccacat ttcccgggcg taatgtaaaa
      781 atctctcccg tcggcatttc cagcttcgcc gctccttgcg ttaacgcatg ccaacgaata
      841 accgacaact ccccggcacc atgtggaagt tgccagtcac ttcctaacac gcaattctta
      901 tcgatcgttc cttgtggagc gttaagcatc aacaaacggc taagagcatc catatcagac
      961 tcctgagtgg tttttggatt acaaacacca acaatcttag tcaataatta atctaatacc
     1021 atcaaatagt taatcatgat ttttgttttg tgattctgat taagaaaaag aaaataccag
     1081 acgataagac aaaatatcaa gcgttgtgaa gaaatgttat ttgctctttt gcgtctatag
     1141 tcatgatgtc aaatgaacgc gtttcgacag gaaatcatca tgaataaata tcaggcagtt
     1201 attattggtt ttggcaaggc tggaaaaaca ttagccgtca cgctggcaaa agcaggttgg
     1261 cgtgtggctc tcatcgaaca atcaaatgca atgtatggcg ggacctgtat taatatcggc
     1321 tgtatcccaa ccaaaacatt ggttcatgac gcacagcagc acacagattt tgtccgtgcc
     1381 atacagcgta aaaatgaagt ggttaatttt ttacgtaata agaattttca taatcttgcg
     1441 gatatgccca atatcgacgt gatcgacggc caggcggagt ttatcaataa tcatagcctg
     1501 cgtgttcatc ggcctgaggg aaatctggaa attcatggcg agaaaatttt tattaatacc
     1561 ggtgcacaaa ccgtggttcc gccaattcct ggaattacca ccacgccagg agtatatgac
     1621 agcaccggat tacttaatct aaaagaattg cctgggcatt taggtatttt gggcggcgga
     1681 tatattggcg ttgagttcgc ctctatgttc gctaattttg gcagcaaagt aaccatttta
     1741 gaggcagctt cgctgttttt gcctcgggaa gatcgggata ttgctgataa tatcgcgacg
     1801 attttacgcg atcagggcgt cgatataatc ctcaatgccc atgtggagcg aatcagtcac
     1861 catgaaaatc aagtgcaagt gcatagcgaa cacgcccaac tggcggtgga tgcactgtta
     1921 atagcttccg gtcgtcaacc ggctaccgct tcgttacatc cagaaaatgc aggtatcgca
     1981 gtaaacgaac gcggggcaat tgtcgttgac aagcaattac ataccaccgc agacaatatt
     2041 tgggcgatgg gagatgttac cggcgggctg caatttactt atatttcact ggatgattac
     2101 cgcattgtgc gtgatgagtt gctgggagaa ggcagacgta gtactgatga tcggaaaaat
     2161 gtgccttatt ccgtattcat gacaccgcca ctgtccaggg ttggtatgac agaagaacag
     2221 gccagagaga gtggtgctga tattcaggtg gtgacattgc ctgtagctgc aattccgcgt
     2281 gccagagtga tgaatgatac tcgtggggta ttaaaagcga ttgttgataa taaaacccaa
     2341 cgtatattag gggcatcact gctgtgtgtt gactcccacg agatgatcaa tatagtgaaa
     2401 atggtgatgg atgccgggct gccttatagc atattacgcg atcagatatt tactcatccg
     2461 tcgatgagcg aatcactcaa tgatctattt tcattagtca aataaactca aaatcagacg
     2521 ccagaacaaa tattctggcg tctcag
//
LOCUS       DAHHTG010000265         2514 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.272, whole genome shotgun sequence.
ACCESSION   DAHHTG010000265 DAHHTG010000000
VERSION     DAHHTG010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2514
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.272"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            158..1699
                     /gene="istA"
                     /locus_tag="NBI74_004599"
     CDS             158..1699
                     /gene="istA"
                     /locus_tag="NBI74_004599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001298859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element ISEc12 family transposase"
                     /protein_id="HCD1934805.1"
                     /translation="MPTVPISMRKLKEILRLKYGVGLSHRQIGRSLAISPSVVSRYAN
                     RAAQLGIKQWPLPTGWDDTKLKHAFLQTQVKMKKHSLPDWATVHRELRNKCVTLQLLW
                     EEYCERNPGGFYSYNHYCRMYREWLKTTSPSMRQVHKAGEKLFVDYCGPTVGVTDPET
                     GEIRTAQVIVAVLGASSYTWAEATWSQQLEDWVMSHVRCFQWLGGVPELVVPDNLKSA
                     TSRACKYDPDVNPTYQQMLEHYNVAVLPARPRKPKDKAKAEVGVQVVERWIMARIRHE
                     IFYSLASLNQRIRELLERLNNKIMQKLGYSRAELFIQLDKPALKPLPEASYSYTLVKK
                     VRVHADYHVEIDKHYYSVPCSLLGQQLEAWISGELVRLFNQGQEVAVHPRKRTYGYST
                     RNEHMPEAHRQHATWTPERLLEWAGHIGSETHSYVLHILNSRPHPEQSYRFCLGLLNL
                     HKKYSKARLNAACARALKTKVWRLSGIKSILEKGLDKQPVQDPKPDLLSTMEHENVRG
                     SEYYH"
     gene            1714..2460
                     /gene="istB"
                     /locus_tag="NBI74_004600"
     CDS             1714..2460
                     /gene="istB"
                     /locus_tag="NBI74_004600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001514323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element ISEc12 family helper ATPase
                     IstB"
                     /protein_id="HCD1934806.1"
                     /translation="MNHLYEQLTALKLTGFRDALKKQLAQPGTYQELGFEERLSLLTA
                     EELTCRENRKAERLIKHARFRLNAELSKLDYRNNRGLDRALIRSLSQGNWLTLKQNIL
                     LTGATGSGKTFLACALGHNACRQGYKVYYYRLKALMEQCYQGHADGRYSKLLTRLNNS
                     DLLLLDDWGLEPLSSEQRSDLLEIVDLMYQRGSIIVVSQLPVENWYKMIGDSTHADAI
                     LDRLVHGSIKIELKGESMRKIQSPLTEGDQ"
BASE COUNT          718 a          590 c          605 g          601 t
ORIGIN      
        1 ccatcgagat cgggtaataa catttgaaca gatcgctgaa taacatcgat ggagatcact
       61 tttgactcat tttgttattc agtgatctcc atcaatgtta ttggaacttc acaggtgtgt
      121 tgatctgtat cttttgccat tccggtaaag gatacctatg ccaacagttc caatttctat
      181 gagaaaactt aaagaaattc ttaggcttaa atacggtgtt ggactcagcc atcgacaaat
      241 tggtcgtagt cttgcaatct ccccttccgt tgtatccaga tatgctaatc gggcggctca
      301 acttggcata aagcagtggc ccttacctac aggatgggat gatacaaaac taaaacatgc
      361 gttccttcag acccaggtta agatgaagaa gcactctctg cctgactggg ctacagtaca
      421 ccgggaactg cgtaataaat gcgtgacgct gcagctactc tgggaagaat actgtgagcg
      481 taatccaggc ggtttttaca gctataacca ttactgccgg atgtaccgtg aatggctcaa
      541 aaccacttca ccatcaatgc gtcaggtaca taaagctggc gaaaaacttt tcgttgatta
      601 ctgtggacct accgttggcg ttaccgaccc tgagaccgga gaaataagaa ctgctcaggt
      661 catcgtagct gttctcgggg catcaagtta cacatgggca gaggccacct ggtctcagca
      721 gcttgaagac tgggtgatga gtcatgttcg ctgcttccag tggttgggtg gcgttcctga
      781 acttgttgtt ccggacaatc tgaaaagcgc cacatccagg gcatgtaagt atgatcctga
      841 cgttaaccct acctaccagc agatgcttga gcattataat gtcgcagttt tgcctgcgcg
      901 gccacgtaaa ccgaaagata aagccaaagc tgaagttggc gttcaggttg ttgaacgctg
      961 gatcatggcc cgaatcaggc atgagatctt ctacagcctt gcatcgctta atcagcgcat
     1021 tcgggagttg ctggaaagac tgaataacaa aataatgcag aagttgggtt attcacgtgc
     1081 agaactcttc atccagcttg ataaacccgc actgaagcct cttcctgaag ccagttacag
     1141 ttacaccctg gtgaagaaag tcagagttca tgccgattac cacgtggaaa tcgacaaaca
     1201 ttactactcg gttccatgtt cgctgttagg ccagcaactg gaagcatgga tctccggaga
     1261 actggtaaga ctcttcaatc aggggcagga ggttgctgtg cacccgcgca agcgtactta
     1321 tggctacagt acccgcaacg agcacatgcc tgaagctcat cgacagcatg ccacctggac
     1381 gccagagcgt cttctggaat gggcggggca cataggcagt gaaactcata gttatgtgct
     1441 tcatatactg aactctcgtc cacatccgga acaaagctat cgcttctgcc ttggactcct
     1501 gaaccttcat aaaaaataca gtaaagccag acttaatgca gcatgtgcaa gagctctgaa
     1561 aacaaaggta tggcgtctgt caggtattaa atcgatcctg gaaaaaggtc tggataaaca
     1621 acctgttcag gatccaaaac cagatctgtt atccacgatg gaacacgaaa acgtacgcgg
     1681 cagtgagtat taccactgat acgggatcca atgatgaatc atctttacga acaactgacc
     1741 gcacttaaac tcaccggctt ccgtgatgcg cttaaaaagc aacttgctca accgggcaca
     1801 taccaggagc tgggcttcga agaacgcctg tcattactga cagcagaaga actaacctgc
     1861 cgtgaaaaca ggaaggcaga gcgtctgatc aaacatgcac ggttcagact taatgctgag
     1921 ttatcaaagc tggattatcg taacaataga gggctggaca gggccctcat ccgttcactc
     1981 agtcagggaa actggttaac cctgaaacaa aatattttac tgaccggggc caccggcagc
     2041 ggtaaaacgt tcctggcatg tgcacttggt cataatgcct gccgacaggg atacaaggtc
     2101 tactattatc gccttaaagc gctgatggaa cagtgctatc aggggcatgc tgatggaaga
     2161 tacagcaaac ttttgaccag gctgaataat agcgatctgc tgcttctgga tgactggggg
     2221 ctggaacctc tctcatcaga acagcgtagc gacctgctgg aaatagtgga tctgatgtac
     2281 caacgaggct caatcatcgt agtgagccag ttgccggtgg aaaactggta caaaatgatc
     2341 ggagactcca cacatgcgga tgccatccta gatcgactgg ttcatggcag tatcaagatc
     2401 gaacttaaag gagaatcaat gcggaaaata caatctccgt tgaccgaagg agatcagtga
     2461 aggtaattta aaaacggttc tgtgaaagtg acacgaaccg atctccatcg atgt
//
LOCUS       DAHHTG010000266         2464 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.273, whole genome shotgun sequence.
ACCESSION   DAHHTG010000266 DAHHTG010000000
VERSION     DAHHTG010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2464)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2464)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2464
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.273"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            complement(<1..1388)
                     /gene="emrY"
                     /locus_tag="NBI74_004601"
     CDS             complement(<1..1388)
                     /gene="emrY"
                     /locus_tag="NBI74_004601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrY"
                     /protein_id="HCD1934807.1"
                     /translation="MAITKSTPAPLTGGTLWCVTIALSLATFMQMLDSTISNVAIPTI
                     SGFLGASTDEGTWVITSFGVANAIAIPVTGRLAQRIGELRLFLLSVTFFSLSSLMCSL
                     STNLDVLIFFRVVQGLMAGPLIPLSQSLLLRNYPPEKRTFALALWSMTVIIAPICGPI
                     LGGYICDNFSWGWIFLINVPMGIIVLTLCLTLLKGRETETSPVKMNLPGLTLLVLGVG
                     GLQIMLDKGRDLDWFNSSTIILLTVVSVISLISLVIWESTSENPILDLSLFKSRNFTI
                     GIVSITCAYLFYSGAIVLMPQLLQETMGYNAIWAGLAYAPIGIMPLLISPLIGRYGNK
                     IDMRLLVTFSFLMYAVCYYWRSVTFMPTIDFTGIILPQFFQGFAVACFFLPLTTISFS
                     GLPDNKFANASSMSNFFRTLSGSVGTSLTMTLWGRRESLHHSQLTATIDQFNPVFNSS
                     SQIMDKYYGSLSG"
     gene            complement(1388..>2464)
                     /gene="emrK"
                     /locus_tag="NBI74_004602"
     CDS             complement(1388..>2464)
                     /gene="emrK"
                     /locus_tag="NBI74_004602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrK"
                     /protein_id="HCD1934808.1"
                     /translation="GAYAYWSMELKDMISTDDAYVTGNADPISAQVSGSVTVVNHKDT
                     NYVRQGDILVSLDKTDATIALNKAKNNLANIVRQTNKLYLQDKQYSAEVASARIQYQQ
                     SLEDYNRRVPLAKQGVISKEALEHTKDTLISSKAALNAAIQAYKANKALVMNTPLNRQ
                     PQVIEAADATKEAWLALKRTDIKSPVTGYIAQRSVQVGETVSPGQSLMAVVPARQMWV
                     NANFKETQLTDVRIGQSVNIISDLYGENVVFHGRVTGINMGTGNAFSLLPAQNATGNW
                     IKIVQRVPVEVSLDPKELMEHPLRIGLSMTATIDTKNEDIAEMPDLASTVTSMPAYTS
                     KALVIDTSPIEKEISNIISHNGQL"
BASE COUNT          735 a          545 c          499 g          685 t
ORIGIN      
        1 cctgaaagcg aaccatagta tttatccata atttgtgacg atgaattaaa cacggggtta
       61 aattgatcga tggttgctgt caactgacta tggtgtaacg attcgcggcg tccccacagt
      121 gtcattgtca acgacgtacc aactgatcct gacaaggtgc gaaaaaaatt actcatactc
      181 gaggcattgg caaatttatt atctggcaag cctgaaaacg aaatcgttgt taagggtaaa
      241 aagaaacagg caacggcgaa tccctgaaaa aactgtggca aaatgatgcc tgtaaaatca
      301 atcgttggca taaatgtcac agaacgccag taatagcaaa ccgcatacat caaaaaacta
      361 aatgtcacca acaaccgcat atcaattttg ttgccataac gtcctatcaa aggtgatatt
      421 aatagcggca tgataccgat gggcgcatag gcaagtccgg cccatatcgc attatacccc
      481 atcgtttcct ggagtaactg cggcataaga actatcgctc cagagtaaaa taaatacgcg
      541 catgtgatac tcacaatacc aatggtgaaa ttacgggact taaacaaact gaggtcaaga
      601 atcggattct ctgaggtcga ctcccaaatg actaaagaga tcagagaaat aactgatact
      661 actgttagta gtattattgt actcgagttg aaccaatcca gatcgcgccc tttatcaagc
      721 ataatttgca agccaccaac accgagcact aacagggtca gtcctggtag attcattttg
      781 accggtgaag tctcagtttc tcgtccttta agtaaggtta agcataatgt caggacgata
      841 atccccatag ggacattgat taaaaatatc caaccccagc taaagttatc gcaaatatag
      901 ccgcccaata tcggcccaca tatcggagcg ataatcacgg tcattgacca taatgccaga
      961 gcaaatgttc ttttttctgg tggataattc cttaataata aactctgtga cagtggaatt
     1021 aacggccccg ccattaaccc ctgaacgact ctaaaaaata tcagcacatc aagattggtc
     1081 gataaactac acattaatga agacagcgaa aaaaaggtta ctgaaagtaa aaataacctt
     1141 aattcgccta ttctttgtgc cagcctgccg gtaacaggga tcgcaatggc atttgctacg
     1201 ccaaacgagg tgataaccca ggtaccttcg tctgttgatg ctcccagaaa gccagatatt
     1261 gtcggtattg cgacgttaga aatagtggaa tccaacattt gcataaacgt cgctaatgac
     1321 aatgcaatag tgacgcacca taacgtccca ccggttaatg gtgcaggagt tgatttagtg
     1381 attgccatta aagttgtcca ttatgcgaaa taatgttgct aatttctttt tctatcggac
     1441 tggtatcgat aactaaagcc ttactggtat aagcaggcat ggacgtcacg gttgaagcca
     1501 gatcgggcat ctcggcaatg tcttcattct tcgtatcaat agttgcagtc atcgataaac
     1561 caatgcgcaa agggtgttcc atgagttctt ttggatccag agaaacttca accggtacac
     1621 gctgaacgat tttgatccag ttccctgtcg cattttgtgc aggtaataag gagaacgcat
     1681 tgccggttcc catattgatc cctgtcaccc gaccatgaaa cacaacattt tcaccataaa
     1741 gatcgctgat aatattgacc gattgaccaa tccgtacatc cgtgagttgt gtttctttaa
     1801 agttggcatt aacccacatt tgacgcgccg gtacgacagc cattaacgat tgtccgggac
     1861 tcactgtttc gccgacctga acacttctct gggcaatata accggtaacc ggactcttaa
     1921 tatccgtacg tttaagcgcc aaccaggctt ctttggttgc atctgcagct tcaatgactt
     1981 gtggctgacg gtttaatggt gtgttcatta ctaaagcttt attcgcttta taagcctgga
     2041 tagcagcatt caatgccgct ttgctactta ttaacgtatc tttggtatgc tccagcgctt
     2101 cttttgaaat aaccccttgc ttcgctaaag gcacacgacg gttataatct tctaaagatt
     2161 gttgatactg aatacgtgct gaagcgactt cggcactgta ttgtttatcc tgtaagtata
     2221 gtttattcgt ttgccgaaca atatttgcca gattattttt agctttattg agtgcgatag
     2281 tggcatcagt tttatccagt gaaactaaaa tgtcaccttg tcgaacgtag ttcgtatctt
     2341 tatgattaac gacagtgaca ctgcctgaga cttgtgcaga aattggatct gcatttcccg
     2401 tgacataggc gtcatctgta ctaatcatgt cttttaattc cattgaccaa taggcatagg
     2461 cacc
//
LOCUS       DAHHTG010000267         2428 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.274, whole genome shotgun sequence.
ACCESSION   DAHHTG010000267 DAHHTG010000000
VERSION     DAHHTG010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2428)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2428)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2428
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.274"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            53..478
                     /locus_tag="NBI74_004603"
     CDS             53..478
                     /locus_tag="NBI74_004603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001373091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence hypothetical
                     protein"
                     /protein_id="HCD1934809.1"
                     /translation="MELQDWRKEPRKNYSNEFKLRMVELASQPGACVAQIARENGVND
                     NVIFKWLRLWQNEGRVSRRLPVTTSSDTGVELLPVEITPDEQKEPVAAIAPSLSTSTQ
                     TRVSASSCKVEFRHGNMTLENPSPELLTVLIRELTGRGR"
     gene            475..825
                     /gene="tnpB"
                     /locus_tag="NBI74_004604"
     CDS             475..825
                     /gene="tnpB"
                     /locus_tag="NBI74_004604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017901265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HCD1934810.1"
                     /translation="MISLPSGTRIWLVAGVTDMRKSFNGLGEQVQHVLNDNPFSGHLF
                     IFRGRRGDTVKILWADADGLCLFTKRLEEGQFIWPAVRDGKVSITRSQLAMLLDKLDW
                     RQPKTSSRNSLTML"
     gene            856..>2428
                     /locus_tag="NBI74_004605"
     CDS             856..>2428
                     /locus_tag="NBI74_004605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66-like element ISEc23 family transposase"
                     /protein_id="HCD1934811.1"
                     /translation="MSQKYLIRIAELERLLSEQAEALRQKDQQLSLVEETEAFLRSAL
                     TRAEEKIEEDEREIEHLRAQIEKLRRMLFGTRSEKLRREVELAEALLKQREQDSDRYS
                     GREDDPQVPRQLRQSRHRRPLPAHLPREIHRLEPEESCCPECGGELDYLGEVSAEQLE
                     LVSSALKVIRTERVKKACTKCDCIVEAPAPSRPIERGIAGPGLLARVLTGKYCEHLPL
                     YRQSEIFARQGVELSRALLSNWVDACCQLMTPVNDALYRYVMNTRKVHTDDTPVKVLA
                     PGQKKAKTGRIWTYVRDDRNVGSSSPPAVWFAYSPNRQGKHPEQHLRPFRGILQADAF
                     TGYDRLFSAEREGGALTEVACWAHARRKIHDVYISSKSATAEEALKRISELYAIEDEI
                     RGLPESERLAVRQQRSKVLLTSLHEWMVEKNGTLSKKSRLGEAFSYVLNQWDALCYYS
                     DDGLAEADNNAAERALRAVCLGKKNFMFFGSDHGGERGALLYGLIGTCRLNGIDPEAY
                     LRHILSVLPEWPSNRV"
BASE COUNT          617 a          603 c          687 g          521 t
ORIGIN      
        1 agacgagaat tggtaactta gacgcccatc tgatatagac ggacatctaa gtatggaatt
       61 acaggactgg cgaaaagaac ctcgtaaaaa ctattcgaat gaattcaaac ttcgtatggt
      121 ggaactggca tcacaacctg gagcttgtgt tgcacagatt gcacgtgaaa atggcgtcaa
      181 tgataatgtt attttcaaat ggctcaggct ctggcagaac gaagggcgtg tttcgcggcg
      241 tcttccggta acgacctctt ctgacactgg cgttgaatta ttacctgtag aaataacgcc
      301 ggatgagcag aaagaacctg tggcggccat tgcgccgtct ttatccactt ccactcagac
      361 cagagtcagt gccagttcct gcaaggtgga attccgtcac ggtaacatga cgctggaaaa
      421 tccatcgcca gagctgctca cagtgttgat ccgtgaactg accgggaggg gaagatgatc
      481 tcactcccat caggtacccg tatctggctc gttgccggcg ttaccgatat gcgtaaatcc
      541 ttcaacggac tgggagaaca ggtacaacat gtgctgaatg ataatccctt ctccggtcac
      601 ctgtttatct tccgtggccg acggggtgac accgtcaaaa ttctttgggc tgatgctgat
      661 ggtctgtgcc tgttcaccaa acgcctggag gaaggccagt ttatctggcc tgcggtacgt
      721 gacggcaagg tatccattac ccgctcgcaa ctggcaatgc tcctcgataa gctggactgg
      781 cgtcagccaa aaacatccag ccgtaactca ctgacaatgt tgtaaaaaac tcctgaccgc
      841 attataaaaa cggtcatgag tcagaaatac ctcattcgca tcgcagagct ggaaaggttg
      901 ctctctgagc aggctgaagc cctccgtcag aaagaccagc aactgagtct ggttgaagag
      961 acggaagcct tcctgcgctc tgcactgaca cgtgccgaag aaaagatcga agaagatgaa
     1021 cgggaaatag aacatctgcg ggctcagata gaaaaactgc gccggatgct gttcggtacc
     1081 cgttctgaaa aactgcgtcg tgaagttgaa ctggctgagg ctctgctgaa acaacgtgaa
     1141 caggacagcg atcgttacag tgggcgggaa gacgatcctc aggttccccg ccagttgcga
     1201 cagtcgcgcc atcgtcgtcc gttaccggca caccttcccc gtgaaataca ccgcctggag
     1261 ccagaagaaa gctgttgccc ggagtgtggc ggtgagctgg attatctggg ggaagtcagc
     1321 gctgaacagc tggaactggt gagcagtgcc ctgaaagtga tccgcacaga acgggtaaaa
     1381 aaagcctgta caaaatgtga ctgtattgtt gaagcaccgg cgccgtcccg cccgatagag
     1441 cgtggtatcg cgggccccgg attacttgcc cgcgtgttaa cgggaaaata ctgcgaacat
     1501 ctgccactgt atcgtcagag tgaaatcttt gcccgccagg gtgtcgaact gagccgggcc
     1561 ttactctcca actgggttga cgcgtgctgc cagttaatga caccggtgaa tgatgccctg
     1621 taccgttatg taatgaatac ccgcaaggtt cacactgatg acacaccggt aaaggtactg
     1681 gcaccgggtc agaaaaaggc gaaaacaggg cgtatctgga cgtatgtccg ggatgatcgc
     1741 aatgtgggtt cgtcatctcc tccagcggtc tggttcgcgt actcgccgaa ccggcagggg
     1801 aaacacccgg agcaacacct ccgccccttc cggggtatcc tgcaggcgga tgcgttcaca
     1861 ggttacgaca ggttgttcag tgcagaacgt gaaggtggtg cactgacaga agttgcgtgc
     1921 tgggcccatg cccggcgaaa aatccacgat gtatacatca gcagcaaaag tgcgacggca
     1981 gaagaagccc tgaagcgaat cagtgaactg tacgccatcg aggatgaaat acggggatta
     2041 ccggagtcag agcgtcttgc cgtcaggcag cagcgaagca aagtgttact gacgtcgctg
     2101 catgaatgga tggtggagaa gaatggtacg ctgtcgaaaa aatccagact gggcgaagcg
     2161 ttcagctatg tactgaatca gtgggatgcc ctctgttatt acagtgatga cggtctggcg
     2221 gaggcggata ataatgctgc ggaaagagcg cttcgtgcag tctgtctcgg aaagaaaaac
     2281 tttatgttct ttggcagcga tcacggcggc gagcgtggag cactgttgta cgggctgatc
     2341 ggcacctgcc gtctgaacgg tatcgatccg gaagcgtatc tgcgccatat cctgagcgta
     2401 ctgccggaat ggccttccaa ccgagttg
//
LOCUS       DAHHTG010000268         2329 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.275, whole genome shotgun sequence.
ACCESSION   DAHHTG010000268 DAHHTG010000000
VERSION     DAHHTG010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2329)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2329)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2329
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.275"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            128..796
                     /locus_tag="NBI74_004606"
     CDS             128..796
                     /locus_tag="NBI74_004606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000115589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HCD1934812.1"
                     /translation="MSVNESEERLLNSTKKTFLSMWSHSNIFYKKGKERKGKERKGKE
                     FCDVMVVFGNDVIIMSDKLINYNIEIDEKIAWNRWYKSAIESSIKQLNGAYNHINSYP
                     DNLYTDAQATEPFSMELPHSDEICIHLIAIANGCSNACYRKYGRYGLNIDTACTGKDT
                     LFTIGIPTRKFVHIFNDSSLDKIFTCLDTTRDFIDYIQARENLLTTSDKYIKIYSINL
                     KMRV"
     gene            complement(780..1817)
                     /locus_tag="NBI74_004607"
     CDS             complement(780..1817)
                     /locus_tag="NBI74_004607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000950586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HCD1934813.1"
                     /translation="MLSNMKIFITEQQKAELERLHDSSRDGRVRDRIKAILLASEGWS
                     SAMIAQALRLHQTTIDHHISEFLNKGKLKPENGGSDSKLSAEQTAFLISQLSDNLFHH
                     TRDVIAFVTRTWNIIFSIPGMNKWLHRNGFTYKKPSGVPHKLSEEKQRQFIEYYKELK
                     TTVGDEPILFIDGVHPTQATKISYGWIRKGQKKAVKTTGSRTRLNIMGALNLKALTSP
                     LICEYKTINEYNVSLFLNEIRKVYPDYNQKIHVILDGAGYHRSQLVKDWAEVVNIRLH
                     YLPPYSPNLNPIERMWKLMNEHARNNRYFSSTREFREAISVFFNQTLPDIADSLTSRI
                     NDHFQVLTPAS"
     gene            1827..>2329
                     /locus_tag="NBI74_004608"
     CDS             1827..>2329
                     /locus_tag="NBI74_004608"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1934814.1"
                     /translation="MLKNQASSMITGIDLVVVEKSTGIVFLCQLKHQELYGADLHAKH
                     VRTTRLKKQASDWLTSMNNWLNSITEIELRKSLQITKHVPKLTTYKLFITKHYAYPLK
                     ELSDEDTAYCNWAQFIYAIQLIDDDKGKRKDSISSLILKLKTLNQEANIEYLHEPTSK
                     WMIKNLT"
BASE COUNT          773 a          422 c          447 g          687 t
ORIGIN      
        1 aaataagtac aatgctttta tgaaaattaa aaccacaaga aaagtataaa aatcatcatt
       61 tactaaaaaa atcaagtgga gataaaaact tcatactata taatatttag aactgaggtt
      121 aaaagatatg tccgttaatg aatcagaaga gaggttgctt aactccacaa agaaaacatt
      181 tctgtcaatg tggtctcaca gcaatatttt ctataagaaa ggaaaggaaa ggaaaggaaa
      241 ggaaaggaaa ggaaaggaat tttgcgatgt aatggtcgtc tttggcaatg atgtaatcat
      301 catgtcagat aaacttatta attataatat tgaaattgac gaaaaaatcg catggaatag
      361 atggtataaa agtgctatcg aaagcagcat aaaacagcta aatggtgcat ataaccatat
      421 aaactcatat cccgacaatc tatatactga tgctcaagca acagagccat tctcaatgga
      481 attaccacat tcggatgaaa tatgcattca tttaattgct atcgcaaatg gttgctctaa
      541 cgcatgctat agaaaatacg gtcgctatgg attaaatatt gatacagcat gtaccggaaa
      601 agacacctta tttactatag gtatacccac aagaaaattc gtccatatat tcaatgatag
      661 cagtctagat aaaatattta catgtttaga tacaaccaga gatttcattg attacattca
      721 agctagggaa aacttactta caacttcaga taagtacatt aaaatatact caataaacct
      781 caagatgcgg gtgtgagcac ctgaaagtga tcgttaattc gggatgtcag tgagtccgct
      841 atatccggca gtgtctggtt aaaaaagaca gatattgctt ccctgaactc tcgcgtactg
      901 ctgaagtagc gattattccg ggcatgttcg ttcattaatt tccacattct ttctatcgga
      961 ttcaggtttg gactgtaagg gggaaggtaa tgcagcctga tatttaccac ttccgcccag
     1021 tctttcacta actgtgaacg gtggtaacct gctccatcca gaataacgtg gattttctgg
     1081 ttataatcag gataaacctt tcgtatctca ttgagaaaaa gggatacatt atattcatta
     1141 atggttttat attcgcagat tagcggtgac gtcagtgctt tcagattcag cgcccccatg
     1201 atattcaggc gtgtgcggct gcctgttgtt tttactgctt ttttctggcc tttgcgtatc
     1261 cagccatagc tgattttagt ggcctgagtc ggatggactc catcaataaa aagaatcggc
     1321 tcatcaccca cagttgtttt cagttcctta taatactcaa tgaattgcct ttgtttctct
     1381 tcactgagtt tatgggggac gcctgacggt tttttgtagg tgaaaccatt acgatgcagc
     1441 cacttattca tccccggaat gctgaaaatg atgttccatg tccgggtgac aaacgcaata
     1501 acatcgcggg tatggtgaaa taaattatcg gataactggc tgattaaaaa ggccgtttgt
     1561 tcagcactga gtttgctgtc tgagccgccg ttttcgggct tcagttttcc tttattcagg
     1621 aattcgctga tatggtgatc aatcgttgtc tgatgcaggc gtaaagcctg agcaatcatg
     1681 gcagaactcc agccttcgga agccagaaga atggctttaa tacgatctct gactcgcccg
     1741 tcacgactgg aatcatgaag gcgttcaagt tcagcttttt gttgctcagt aatgaatatt
     1801 ttcatgttgg acagcatgat cttctgttgc tgaaaaatca agcatcttca atgatcacgg
     1861 gtatagactt agttgttgtc gaaaaaagca caggaatagt ttttctttgt caattaaaac
     1921 atcaagaact ttatggtgct gatctacatg caaaacatgt tcgaaccact agattaaaaa
     1981 aacaagcgtc ggattggctt acttcgatga acaattggtt aaacagcata accgaaatag
     2041 aactaagaaa atcgttgcaa ataacaaaac atgtaccaaa attaacaaca tataaattat
     2101 tcataacaaa acattatgcc tatccactca aagagctatc agatgaagat acagcgtatt
     2161 gtaactgggc gcagtttatt tatgccattc aacttatcga cgatgataag gggaaaagaa
     2221 aagatagtat ttcatcttta atcctcaaat taaaaacttt aaatcaggaa gctaacattg
     2281 aatatcttca tgagcctaca tcgaaatgga tgataaaaaa tttaacttt
//
LOCUS       DAHHTG010000269         2299 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.277, whole genome shotgun sequence.
ACCESSION   DAHHTG010000269 DAHHTG010000000
VERSION     DAHHTG010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2299)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2299)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2299
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.277"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            complement(41..676)
                     /gene="msrQ"
                     /locus_tag="NBI74_004609"
     CDS             complement(41..676)
                     /gene="msrQ"
                     /locus_tag="NBI74_004609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase
                     heme-binding subunit MsrQ"
                     /protein_id="HCD1934815.1"
                     /translation="MRLTAKQVIWLKVCLHLAGLLPFLWLVWAINHGGLGADPVKDIQ
                     HFTGRTALKFLLAALLITPLARYAKQPLLIRTRRLLGLWCFAWATLHLTSYALLELGV
                     NNLALLGKELITRPYLTLGIISWVILLALAFTSTQSMQRKLGKHWQQLHNFVYLVAIL
                     APIHYLWSVKIISPQPLIYAGLAVLLLALRYKKLLSLFNRLRKQAHNKLSL"
     gene            complement(677..1681)
                     /gene="msrP"
                     /locus_tag="NBI74_004610"
     CDS             complement(677..1681)
                     /gene="msrP"
                     /locus_tag="NBI74_004610"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase catalytic
                     subunit MsrP"
                     /protein_id="HCD1934816.1"
                     /translation="MKKNQFFKESDVTAESVFFMKRRQVLKALGISAAAFSLPHAAHA
                     DLLSWFKGNDRPPAPAGKPLEFSKPAAWQNNLPLTPADKVSGYNNFYEFGLDKADPAA
                     NAGSLKTEPWTLKISGEVAKPLTLDHDDLTRRFPLEERIYRMRCVEAWSMVVPWIGFP
                     LHKLLALAEPTSNAKYVAFETIYAPEQMPGQQDRFIGGGLKYPYVEGLRLDEAMHPLT
                     LMTVGVYGKALPPQNGAPVRLIVPWKYGFKGIKSIVSIKLTRERPPTTWNLAAPDEYG
                     FYANVNPHVDHPRWSQATERFIGSGGILDVQRQPTLLFNGYADQVASLYRGLDLRENF
                     "
     gene            complement(1790..2203)
                     /gene="hiuH"
                     /locus_tag="NBI74_004611"
     CDS             complement(1790..2203)
                     /gene="hiuH"
                     /locus_tag="NBI74_004611"
                     /EC_number="3.5.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyisourate hydrolase"
                     /protein_id="HCD1934817.1"
                     /translation="MLKRYLVLSVATAAFSLPSLVYAAQQNILSVHILNQQTGKPAAD
                     VTVTLEKKADNGWLQLNTAKTDKDGRIKALWPEQTATTGDYRVVFKTGDYFKKQNLES
                     FFPEIPVEFHINKVNEHYHVPLLLSQYGYSTYRGS"
BASE COUNT          607 a          550 c          558 g          584 t
ORIGIN      
        1 atccggccgg gataacgcga agtttgtcgg caatatgaag ttatagagat aatttgttgt
       61 gggcctgctt gcgtaaccgg ttgaaaaggg aaagcaactt cttataccgt aaggctaaaa
      121 gcagtacagc cagcccagcg tagatgagtg gctgcggtga aataatcttc actgaccaca
      181 gataatgtat tggggccagg atcgcgacaa gatagacgaa gttatgcaac tgttgccagt
      241 gtttgccgag ttttcgctgc atcgactggg ttgaagtgaa cgctaaagca agcaaaataa
      301 cccagctgat aattcctaac gttaaataag gccgggtaat taactcctta cctaataacg
      361 ccagattatt cacgcccaac tccagcaatg cgtaactggt taaatgcagt gtcgcccagg
      421 caaagcacca taagcctaac aggcggcgag tgcgtatcaa taacggctgt tttgcgtagc
      481 gtgccagagg tgtgattaac aaggccgcaa gcaaaaattt cagtgcagtg cgaccggtaa
      541 aatgctgaat atctttcacc ggatcggcac ctagtccacc gtgattgatc gcccagacca
      601 gccagagaaa cggcaacaat ccggcaagat gcaggcaaac tttcagccat atcacctgct
      661 ttgctgtcag acgcatttag aaattctccc gcaaatccag gccacgatac agcgatgcca
      721 cctggtcggc gtaaccatta aacagtagtg ttggctggcg ctgaacatcg agaatgccgc
      781 ctgaaccaat aaatcgttcg gtagcctgtg accagcgcgg gtgatcaaca tgcggattaa
      841 cgttggcgta aaaaccgtat tcgtcaggcg ctgccagatt ccaggtggtt ggcggacgct
      901 cgcgggtcag cttaatactg acgatcgatt taatcccttt aaagccatat ttccacggca
      961 caatcagtcg caccggtgcg ccattttgcg gtggtaacgc cttgccataa acacctacgg
     1021 tcatcagtgt gagcggatgc attgcttcgt cgagacgcaa tccttcgaca taaggatatt
     1081 tcagcccgcc gccgataaag cggtcctgct ggcctggcat ctgttccggc gcataaatcg
     1141 tttcgaaagc gacatatttc gcattgctgg tgggttcggc aagcgccagc aatttgtgta
     1201 gcggaaaacc aatccacggc accaccatcg accacgcttc cacgcagcgc atacgataaa
     1261 tacgctcttc cagcgggaag cgacgggtta aatcatcatg atcgagggtc aatggttttg
     1321 ccacttcgcc gctgattttc agtgtccatg gctcggtttt caggctacca gcattagcag
     1381 cgggatcggc tttatccagc ccgaattcat agaagttgtt ataaccggag actttatctg
     1441 ctggcgtcag tggcaggtta ttttgccagg cggcaggctt gctgaactcc agcggttttc
     1501 cggcgggggc gggcggacga tcgttccctt taaaccagct aagcagatcg gcatgcgcag
     1561 cgtgaggcaa agaaaatgca gctgcgctga tgcccagtgc ttttaacacc tgccgacgct
     1621 tcataaagaa taccgactcg gccgtaacat ctgattcttt aaaaaattga ttctttttca
     1681 taagccatca cacttttttg tcgttagcca acaagagccg tttactgtta atctgtcagg
     1741 gagtcacctc cctaacagta gcagagatca gcttttacat atttacgcat taactgccac
     1801 gataggttga atacccatat tggctgagta acaaaggcac atgataatgc tcgttcactt
     1861 tattaatatg aaattcaacg gggatctcag ggaagaaact ttcaagattt tgtttcttga
     1921 aatagtcccc ggttttaaat acgacacggt aatcgccagt agtcgcagtt tgctcgggcc
     1981 acagtgcctt aattcgtcca tccttatctg ttttggcggt attaagttgt aaccagccgt
     2041 tatccgcttt cttttcaaga gtgactgtca cgtcggcagc gggttttcct gtttgctggt
     2101 tcaaaatgtg cacgctaaga atgttttgtt gtgcggcata aaccaaagaa ggtaatgaaa
     2161 atgctgccgt tgctaccgag agtactaaat aacgctttaa catgtttata tccttgtcat
     2221 gtgaatgagt tttgaggata gcgaaaaaac gtccagggta accttacagc agcattacaa
     2281 ttttgtaatg aaccaggtt
//
LOCUS       DAHHTG010000270         2133 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.278, whole genome shotgun sequence.
ACCESSION   DAHHTG010000270 DAHHTG010000000
VERSION     DAHHTG010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2133)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2133)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2133
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.278"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            68..451
                     /locus_tag="NBI74_004612"
     CDS             68..451
                     /locus_tag="NBI74_004612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1934818.1"
                     /translation="MSVLYIQIRRNQITVRDLESKREVSGDAAFSNQRLLIANFFVAE
                     KVLQDLVLQLHPRSTWHSFLPAKRMDIVVSALEMNEGGLSQVEERILHEVVAGATLMK
                     YRQFHIHAQSVVLSDSAVMAMFKQK"
     gene            complement(515..958)
                     /locus_tag="NBI74_004613"
     CDS             complement(515..958)
                     /locus_tag="NBI74_004613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000236991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HCD1934819.1"
                     /translation="MVISIRRSRHEEGEELVAIWCRSVDATHDFLSAEYRAELEELVH
                     SFLPEAPLWVAVNERDQPIGFMLLSGQHMDALFIDPDVRGCGVGRMLVKHALSMAPEL
                     TTTVNEQNEQAVGFYKKVGFKVTGRSEVDDLGKPYPLLNLAYVGV"
     gene            1115..2044
                     /gene="metA"
                     /locus_tag="NBI74_004614"
     CDS             1115..2044
                     /gene="metA"
                     /locus_tag="NBI74_004614"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HCD1934820.1"
                     /translation="MPIRVPDELPAVNFLREENVFVMTTSRASGQEIRPLKVLILNLM
                     PKKIETENQFLRLLSNSPLQVDIQLLRIDSRESRNTPAEHLNNFYCNFEDIQEQNFDG
                     LIVTGAPLGLVEFNDVAYWPQIKQVLEWSKDHVTSTLFVCWAVQAALNILYGIPKQTR
                     TDKLSGVYEHHILHPHALLTRGFDDSFLAPHSRYADFPAALIRDYTDLEILAETEEGD
                     AYLFASKDKRIAFVTGHPEYDAQTLAQEYFRDVEAGLDPEVPYNYFPHNDPQNKPRAS
                     WRSHGNLLFTNWLNYYVYQITPYDLRHMNPTLD"
BASE COUNT          545 a          560 c          475 g          553 t
ORIGIN      
        1 gaaaaacccc ggtcaataag gccggggttt tttgcatatc aattatttgc atgatgaagg
       61 gaatctcatg tcagttctgt atatccaaat tcgtcgtaat caaattactg ttcgcgatct
      121 tgaaagcaaa cgtgaagtgt caggagatgc tgccttcagt aaccagcgcc tgttaatcgc
      181 caatttcttt gttgcagaaa aagttctgca agatcttgtt ctgcaactcc acccacgttc
      241 aacctggcat tcttttttgc cagcaaaacg tatggatatt gttgtgagcg cgctggaaat
      301 gaatgagggc ggtttgtcac aggttgagga acgcattctt catgaagtgg tcgcaggggc
      361 aacgttaatg aaatatcgcc agttccacat ccatgcgcaa tcagtggtac tcagtgatag
      421 tgcggtcatg gcaatgttta agcagaaata atcatgtcac cattggtggg tacgaaacct
      481 aaagttcagc ccaccgggat gagaaaaaaa ccgcctacac ccccacatat gccagattca
      541 gcaacggata cggtttcccc aaatcgtcca cctcagagcg tcccgtaacc ttaaagccca
      601 ccttcttata gaacccaacc gcctgctcat tttgctcatt aacagtggtt gtcagttccg
      661 gggccattga gagcgcatgc ttcaccagca tccggcccac gccgcagccg cgcacatcag
      721 gatcgataaa cagcgcatcc atatgctgcc cacttaacaa cataaatcca atcggctgat
      781 cccgctcatt aaccgcgacc cacaacggtg cttccggcag aaaggaatgc accagctctt
      841 ccagctcggc ccgatactct gctgataaaa aatcgtgagt ggcatcgaca gaacgacacc
      901 aaatcgcaac gagttcctcc ccctcctcat gccgtgagcg tcgaatacta ataaccattt
      961 tctctccttt tcgtcattct tatattctaa cgtagccttt tccttgaaac tttctcacct
     1021 tcaacatgca ggctcgacat tggtaaattt tctggttatc ttcagctatc tggatgtcta
     1081 aacgtataag cgtatgtagt gaggtaatca ggttatgccg attcgtgtgc cggacgagct
     1141 acccgccgtc aatttcttgc gtgaagaaaa cgtctttgtg atgacaactt ctcgcgcgtc
     1201 tggtcaggaa attcgtccgc taaaggtgct tatccttaac ctgatgccga agaagatcga
     1261 aactgaaaat cagtttctgc gcctgctttc aaactcacct ttgcaggtcg atattcagct
     1321 gttgcgcatc gattcccgtg aatcgcgcaa cacgcccgca gagcatctga ataacttcta
     1381 ctgtaacttt gaagatattc aggaacaaaa ctttgacggt ctgatagtta ccggcgcgcc
     1441 gctgggcctg gtggagttta atgatgtcgc ttactggccg cagatcaaac aggtgctgga
     1501 gtggtcgaaa gatcacgtca cctcgacgct gtttgtctgc tgggcggtac aggccgcgct
     1561 aaatatcctc tacggcattc ctaagcaaac tcgcaccgac aaactctctg gcgtttatga
     1621 gcatcatatt ctccatcctc atgcgcttct gacgcgtggc tttgatgatt cattcctggc
     1681 accgcattcg cgctatgctg actttccggc agcgttgatt cgtgattaca ccgatctgga
     1741 aattctggca gagacggaag aaggggatgc atatctgttt gccagcaaag ataagcgaat
     1801 tgcctttgtg acgggccatc ccgaatatga tgcgcaaacg ctggcgcagg aatatttccg
     1861 cgatgtggaa gccggactag acccggaagt accgtataac tatttcccgc acaatgatcc
     1921 gcaaaacaaa ccgcgagcga gctggcgtag tcacggtaat ttgctgttta ccaactggct
     1981 caactattac gtctaccaga tcacgccata tgatctacgg cacatgaatc caacgctgga
     2041 ttaatcttct gtgatagtcg atcgttaagc gattcagcac cttacctcag gcaccttcgg
     2101 gtgccttttt tatttccgaa acacacctca gta
//
LOCUS       DAHHTG010000271         2067 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.279, whole genome shotgun sequence.
ACCESSION   DAHHTG010000271 DAHHTG010000000
VERSION     DAHHTG010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2067)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2067)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2067
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.279"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            20..307
                     /locus_tag="NBI74_004615"
     CDS             20..307
                     /locus_tag="NBI74_004615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001825190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1934821.1"
                     /translation="MSTRNIHVNTASYTLLVAGKKKNTGEEWDVLEFSSLTELKKYRK
                     SHPEKMAFSYSYALSQGVDKQFRHINIAEVDHFKQFLRQIKRAGLDIRAIC"
     gene            428..1249
                     /locus_tag="NBI74_004616"
     CDS             428..1249
                     /locus_tag="NBI74_004616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF945 domain-containing protein"
                     /protein_id="HCD1934822.1"
                     /translation="MRLASRFGRYNSIRRERPLTDDELMQFVPSVFSGDKHESRSERY
                     TYIPTINIINKLRDEGFQPFFACQSRVRDLGRREYSKHMLRLRREGHINGQEVPEIIL
                     LNSHDGSSSYQMIPGIFRFVCTNGLVCGNNFGEIRVPHKGDIVGQVIEGAYEVLGVFD
                     KVTDNMEAMKEIHLNSDEQHLFGRAALMVRYEDENKTPVTPEQIITPRRREDKQNDLW
                     TTWQRVQENMIKGGLSGRSASGKNTRTRAITGIDGDIRINKALWVIAEQFRKWKS"
     gene            complement(1546..>2067)
                     /locus_tag="NBI74_004617"
     CDS             complement(1546..>2067)
                     /locus_tag="NBI74_004617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000614282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HCD1934823.1"
                     /translation="RGGKMKKWMLAICLMFINEICQATDCFDLAGRDYKIDPDLLRAI
                     SWKESRYRVNAIGINPVTGYGSGLMQVDSQHFNELARYGIKPEHLTTDPCMNIYTGAY
                     YLAIAFKKWGVTWEAVGAYNAGFRKSERQNQRRLAYASEVYRIYTGIKSSKGIRLPAT
                     KKSLPEINSVQNN"
BASE COUNT          550 a          429 c          504 g          584 t
ORIGIN      
        1 agagagagga gaaagcagga tgagtacccg aaatatccac gttaacaccg cgtcgtacac
       61 gcttcttgtt gccgggaaga aaaaaaacac cggggaagag tgggacgtcc tggaattcag
      121 cagcctgact gagctgaaaa aatatcgcaa aagccacccg gaaaagatgg ccttcagtta
      181 tagctacgcg ctcagtcagg gtgtggataa gcagttccgc catatcaaca ttgcggaagt
      241 cgatcacttc aaacagttcc tgcgtcagat taagcgtgcc ggcctcgata tccgggcgat
      301 ctgctgaccg gatgtgatga atatctctcc tgattgttgc aatcttgtta atggagatcg
      361 ggggggggat gatattaagc ggaaaaattt tttattaaat ttattctttc ataaaggagt
      421 agctgttatg cgattagctt cccgttttgg tcggtataat tccatccgcc gtgaacgtcc
      481 tttaacggat gatgaattaa tgcagttcgt gccttcggta ttttccggtg ataaacatga
      541 gtcccggagt gaacgttata cgtatattcc aacaatcaat atcatcaata agttacgtga
      601 tgaaggtttc cagccattct ttgcctgtca gagtcgggtt cgtgatttgg gacgtcgcga
      661 atacagtaaa catatgttac gtcttcgcag ggaagggcat attaacggac aggaagttcc
      721 tgaaattatc ctgcttaatt cacatgatgg ttcatccagt tatcagatga tccccggaat
      781 ttttcgtttt gtctgcacaa atggcctggt gtgcggaaat aattttggcg aaatccgcgt
      841 tccacataaa ggtgatattg tcgggcaggt tatcgaggga gcgtatgaag tgctcggtgt
      901 ctttgataag gtcactgata atatggaggc gatgaaagaa attcatctta acagtgacga
      961 gcaacattta tttggcagag ctgcactgat ggtcaggtat gaagatgaaa ataaaacgcc
     1021 agtgacacct gaacagataa ttactccccg tcgtcgggaa gacaaacaga acgatctctg
     1081 gacaacctgg cagcgggttc aggagaatat gataaaaggt ggattatcgg ggcgaagtgc
     1141 ctccgggaaa aataccagga caagagccat tacaggtatt gatggtgata taagaatcaa
     1201 caaggcgtta tgggtgattg ccgaacagtt cagaaagtgg aagtcatgat aatataagta
     1261 aagccccgaa aaaattttcg gggctttact tatattatca tttttcggca ttgctgtgtc
     1321 gtctgatata cctcaatttc atcgaccggc tttccggttg tctgtaaata ttgtgcccgc
     1381 gaaaaatggt ctggcaggag gctgcgcagt taccgggtat atataagatg ggttcccgct
     1441 tcgcggggct tcggccagtc agtattgtca ctcatatgat atttttgtgt gtggccttcc
     1501 atgctgcttt tgcggcataa ccggtatctg acaatgtctg taagattaat tattctgcac
     1561 gctgttaatt tccggaagtg atttcttcgt ggccggaagc cggatacctt tactgctttt
     1621 tatcccggta taaatccggt aaacctctga tgcgtaggca agacgtctct ggttctggcg
     1681 ttcggatttc ctgaatcctg cattgtatgc accaacggcc tcccaggtga cgccccattt
     1741 tttaaatgct attgccagat aataagcacc ggtataaatg ttcatgcagg gatctgttgt
     1801 cagatgttct ggcttaattc cgtagcgggc cagttcgtta aaatgctggg aatctacctg
     1861 catcagtccg ctgccatatc ccgttaccgg attaataccg atggcattaa cccggtaacg
     1921 ggattctttc catgatattg ctctcagtaa atcaggatct attttgtaat cccggcctgc
     1981 aagatcaaag caatcagtgg cctggcagat ctcatttata aacatcaggc agatggctaa
     2041 catccatttt ttcatttttc cacctct
//
LOCUS       DAHHTG010000272         2040 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.280, whole genome shotgun sequence.
ACCESSION   DAHHTG010000272 DAHHTG010000000
VERSION     DAHHTG010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2040)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2040)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2040
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.280"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            <1..78
                     /gene="ariR"
                     /locus_tag="NBI74_004618"
     CDS             <1..78
                     /gene="ariR"
                     /locus_tag="NBI74_004618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm/acid-resistance regulator AriR"
                     /protein_id="HCD1934824.1"
                     /translation="TSDILKADVIRKTLEIVLRYTADDM"
     gene            191..439
                     /locus_tag="NBI74_004619"
     CDS             191..439
                     /locus_tag="NBI74_004619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1934825.1"
                     /translation="MNNSIPERFIFQCALFKNLEKEVFMTHGYVDSHIIAHALRLRLK
                     DETSVILSDLYLQILQYIEMHKTTLTDIIINDRESVLS"
     gene            737..1630
                     /locus_tag="NBI74_004620"
                     /pseudo
     CDS             737..1630
                     /locus_tag="NBI74_004620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415686.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
     gene            1762..1980
                     /locus_tag="NBI74_004621"
     CDS             1762..1980
                     /locus_tag="NBI74_004621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001065870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1934826.1"
                     /translation="MNNSNNLDYFTLYIIFSIAFMLITLLVILIAKPSTGLGEVLVTI
                     NLLNALVWLAINLVNRLRERLVSHRDQH"
BASE COUNT          553 a          392 c          436 g          659 t
ORIGIN      
        1 acaagtgata ttctcaaagc tgatgtgatt agaaaaacac tggaaatcgt gttgcgatac
       61 acagctgatg atatgtaact tttaattata aagtataagt ttatagaaaa ctcattcatc
      121 ttatttttgt ctgtcgcttt agactataca tagcataatt ttattgggtc actattcctg
      181 cgaggctgac atgaataatt caatcccaga gagatttatt tttcaatgtg ctttgtttaa
      241 aaacctcgag aaggaggtgt tcatgacaca tggatatgtt gatagtcata ttattgctca
      301 tgctttacgg ttgcgtttaa aagatgagac cagtgttatt ctttctgatc tctatctgca
      361 aatcttgcag tacattgaaa tgcataagac tacactaacg gatatcatta ttaatgacag
      421 ggaatccgtg ctctcttaaa acttgagtaa ggtttttgaa taaacatcag ctgtatcact
      481 atgctgatgc aaagtgagca tttcaggcgt tatgctttct taatatatcc gcaatatcag
      541 agggcaaaca ggaatggaga gcgctcgttt tccattcttg acgcctgata tcccgcctaa
      601 tttgtttaat tctgcctaca ttgttctaaa taattaatag gttagatgaa gttggagaaa
      661 attttatctt cggcgtggat atttgtgatg ctctctacca tgctatctgt agaccccagc
      721 gcgagcgaat gaacgtctct ttagcttcta cagacttatc gataaaggct ttgggatttt
      781 gatatttatt ctgttaatcg cctgcgccgc tgccttcctt cttgataggt atttcaataa
      841 aagcgcaacg cctgaagaga tcctgcgacg ggctataaat aatggggaga tcgtcccttt
      901 ttaccaacct gtggtaaatg gtcgggaagg gacattgcgg ggagttgagg tgttagcccg
      961 ctggaaacaa cctcacggtg gatatatatc acctgcggca tttattccac ttgctgaaaa
     1021 atgcggatta atcgttccgc ttacgcaaag cctgatgaat caggttgcca cccagatgaa
     1081 cgctatcgcg agtaaactgc cggaaggttt tcatattggg attaatttta gcgcctcgca
     1141 tattgtttcg ccgacgtttg tcgacgagtg cttaaattac cgtgacagtt ttacccgccg
     1201 cgatttaaac cttgttctgg aagtcaccga gcgtgaacca ttaaatgttg atgaaagtct
     1261 ggttcagcgg ttgaacattc tgcatgaaaa tggttttgtc atcgcgctgg atgatttcgg
     1321 tactggttac tcagggcttt cttatctgca tgacctgcat attgattata taaaaattga
     1381 tcacagtttc gttggccgcg tcaacgcaga cccagaatca acccgaattc tggattgtgt
     1441 attggatctg gcgcgtaaac tttcgatcag tatcgtcgct gaaggtgtcg aaacgaaaga
     1501 acaacttgac tatctgaacc aaaataatat tacatttcag cagggttatt atttctataa
     1561 acctgttaca tacatcgacc tggtcaaaat tatcctttct aaaccgaagg tgaaggttgt
     1621 ggttgagtga aaattgatca gtaaggccat ggtacggtgt aattatagac agctaattag
     1681 ctcgttgcct cttgttacta ttgttcattc ttttgtttgc tataattgtt tgaaagtttt
     1741 gactggattg ccattagtag catgaacaat agtaataatc tggattattt cactctctat
     1801 atcatctttt ccattgcatt tatgttgatc actctcctgg tcatccttat tgcaaaaccc
     1861 agcaccgggc tgggagaagt gcttgtgacg ataaatttgc ttaatgccct tgtttggctg
     1921 gcgatcaatc tggttaatcg attaagggaa agactcgtca gccacaggga tcagcactaa
     1981 tctttcagtt tctcactgtc agtatgcggc tgaatgggtt gctggcagtg aacccctgga
//
LOCUS       DAHHTG010000273         2030 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.281, whole genome shotgun sequence.
ACCESSION   DAHHTG010000273 DAHHTG010000000
VERSION     DAHHTG010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2030)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2030)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2030
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.281"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            60..757
                     /locus_tag="NBI74_004622"
                     /pseudo
     CDS             60..757
                     /locus_tag="NBI74_004622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            1029..1580
                     /gene="papX"
                     /locus_tag="NBI74_004623"
     CDS             1029..1580
                     /gene="papX"
                     /locus_tag="NBI74_004623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001298062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator PapX"
                     /protein_id="HCD1934827.1"
                     /translation="MRACTQTVCFSNMENNEMNNTDTLEKIIRHQKNKDPAYPFREHL
                     LMQLCIRTNKRMQDNISEFLGVYGINHSAYMVLTTLFAAENHCLSPSEISQKLQFTRT
                     NITRITDFLEKAGYVKRTDSREDRRAKKISLTSEGMFFIQRLTLAQSMYLKEIWDYLT
                     HDEQELFEVINKKLLAHFSDASS"
BASE COUNT          593 a          429 c          482 g          526 t
ORIGIN      
        1 attgggtgat gctgccaact tactgattta gtgtatgatg gtgtttttga ggtgctccag
       61 tggcttctgt ttctatcagc tgtccctcct gttcagctac tgacggggtg gtgcgtaacg
      121 gcaaaagcac cgccggacat cagcgctatc tctgctctca ctgccgtaaa acatggcaac
      181 tgcagttcac ttacaccgct tctcaacccg gtacgcacca gaaaatcatt gatatggcca
      241 tgaatggcgt tggatgccgg gcaacagccc gcattatggg cgttggcctc aacacgattt
      301 tacgtcactt aaaaaactca ggccgcagtc ggtaacctcg cgcatacagc cgggcagtga
      361 cgtcatcgtc tgcgcggaaa tgggcgaaca gtggggctat gtcggggcta aatcgcgcca
      421 gcgctggctg ttttacgcgt atgacaggct ccggaagacg gttgttgcgc acgtattcgg
      481 tgaacgcacg atggcgacgc tggggcgtct tatgagcctg ctgtcaccct ttgacgtggt
      541 gatatggatg acggatggct ggccgctgta tgaatcccgc ctgaagggaa agctgcacgt
      601 aatcagcaag cgatatacgc agcgaattga gcggcataac ctgaatctga ggcagcacct
      661 ggcacggctg ggacggaagt cgctgtcgtt ctcaaaatcg gtggagctgc atgacaaagt
      721 catcgggcat tatctgaaca taaaacacta tcaataagtt ggagtcatta cctacaacgt
      781 ttatcgacct cacacgaaca acgatggttt accgccttgc ttacatgaag aaaagtggaa
      841 acaggttatt ccggtgtcct gattttgtga accactacaa taatcctttt tcacagactt
      901 gtcagcagcc agcatttatg ttcttttacc tgcgggaatt tatccgtctg cctgtcggga
      961 ctactctgtc atacagaaat caggtaggaa taaattgtag tggaaagtcg atgtttaccg
     1021 gatgactgat gcgcgcttgt acacagacag tgtgtttcag taatatggag aataatgaaa
     1081 tgaataacac agacacatta gaaaaaataa tcagacacca aaaaaacaaa gaccccgcat
     1141 atccctttcg ggaacatttg ttgatgcagc tctgtattcg cacaaataaa agaatgcagg
     1201 ataatatatc tgaatttctg ggggtgtatg gaataaatca ctcagcatat atggttctca
     1261 ccacattatt cgcagcggag aaccattgtc tgtcaccttc agagataagc cagaaacttc
     1321 agtttaccag aactaatatt acccgcatta cagatttttt agaaaaagcc ggatatgtaa
     1381 aaaggacgga tagcagggag gatcgccgtg ctaaaaaaat cagtctgaca tctgaaggta
     1441 tgttttttat tcagaggctc actcttgcac aaagcatgta tctgaaagaa atctgggatt
     1501 atctgaccca tgatgaacag gaactgtttg aagtcattaa taaaaagtta ctggcacatt
     1561 tttctgatgc cagctcataa agtgcgaaat atctgaggat gctggatagc ttcaggcaag
     1621 agaataatga ttcttgcaga tgtgtttttc cggatacaaa aacaaaagta aaaaattgca
     1681 gcgccaggcc acctttcaaa gcagggaggc ctgtaccgcg tcgaaaattt cagccagtta
     1741 atgtaattgt ctgaaccagg cacttcgccc gggcaggaga aggagcgtgg ttgcggtcat
     1801 cctgtatact gaagtcgtta tgaaggaata atacaaataa ttgcccgttt tctctgtacg
     1861 atagaggcag gtgtgtatga ttgcattact atgtttttgt gatataaaaa actaaatggt
     1921 gagatatgca ttttaaaata taacaaaaat caccatgaaa taagaataat tatccacgga
     1981 gaacataaac tgcagactgt tacaatgcat gctgttcatc ttgacaatgt
//
LOCUS       DAHHTG010000274         2012 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.282, whole genome shotgun sequence.
ACCESSION   DAHHTG010000274 DAHHTG010000000
VERSION     DAHHTG010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2012)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2012)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2012
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.282"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            368..613
                     /locus_tag="NBI74_004624"
     CDS             368..613
                     /locus_tag="NBI74_004624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001665004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1934828.1"
                     /translation="MLDDAAQRVARGGIQNPLAYLLAMLRKAQGGEFNQYRRGKATVP
                     EFVPHVVSEPKIPAEPRKKRDISKLVAEIRAQCLEKK"
     gene            complement(664..1740)
                     /locus_tag="NBI74_004625"
     CDS             complement(664..1740)
                     /locus_tag="NBI74_004625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001479750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HCD1934829.1"
                     /translation="MNWNAETPYNDLPILPPDLERIETRSVLKACISARAAIAELKTA
                     GELIPDQGLLINILPMLEAKDSSRIENIVTTSDQLFQYADRADGADPATKEALRYRTA
                     LYDGYTHLEDYPLCTNTAVAICTKLRAVQTDIRKTPGTVLRDQNNNVVYTPPVGEDSI
                     RELLANWERFIHGDDDLDPLVKMAIAHYQFECIHPFPDGNGRTGRILNILYLIQSELL
                     SLPILYLSRFILERRDDYYTLLRRVTEEGDWESWILFMLEAVESTSRWTTDKISIVRA
                     LMAETTEYVREKLPKIYTHELVQALFAQPYCRIDNLVERGVAKRQTASTYLKQLVEIG
                     VLEEMSVGREKLYINTRLLQELNQ"
BASE COUNT          528 a          518 c          462 g          504 t
ORIGIN      
        1 cattcgcaag gacaccagcc tgcgttattt atcgtcgcgt atagaactgc tggaagaacg
       61 cgcccgctgg cagcaacgcc agtctcctga ggagactaag ccagaaacgt ccggtctcaa
      121 tacggtacca ggcaaaattt cacctggtac cgaatcagga ctaagccgga aaccgaggcc
      181 tggcggctta gtctcaaatc aggaccgggc ttctgtacgt actgtatcta cagatattcg
      241 taatagtact accgcgcgcg cgaacagctc accgccgaac tggccgccgg aaattccgtt
      301 aactaatcct gaaaaacagc ttgtcttgca ggccatgcag aacctgccgc ccccgctgcg
      361 ccaggacgtg cttgatgacg cagctcaacg cgttgcccgt ggcggcatac aaaacccgct
      421 cgcctatctg ctggcaatgc tgcgcaaagc gcagggcggt gagtttaatc agtatcgacg
      481 cggcaaagcg acggtgccgg agtttgtgcc gcatgtggtt tcagagccga aaataccggc
      541 tgaaccgcgt aaaaaacggg atatcagtaa gttggttgcg gagattcggg cgcagtgttt
      601 ggagaaaaag tgatgccagt ggcatcacca tttgtccacg cttttgtttt ctgggcccat
      661 tcactactga ttcaactcct gtagcaacct agtgttaata tacagctttt cacggccaac
      721 gctcatttcc tccaacacac caatttcgac caactgcttg aggtatgtcg atgccgtctg
      781 gcgcttggcc accccacgtt cgacaaggtt atcgatccga caatatggtt gtgcaaaaag
      841 cgcctggaca agttcatggg tgtagatttt cggtagcttc tcgcgtacat actctgtggt
      901 ttcagccatt aatgcccgca caatcgatat cttatcagta gtccatcggg acgtactctc
      961 cacagcctcc agcataaata atatccacga ttcccagtca ccctcttctg tcaccctacg
     1021 aaggagcgtg tagtaatcat cgcgtctctc caagataaag cgggataaat acaggattgg
     1081 tagcgaaagc aattcgctct ggatcagata tagaatattc aagatacggc ccgttctccc
     1141 gttcccatcc gggaacgggt gaatgcactc aaactgatag tgcgcaatag ccattttaac
     1201 caatggatct aaatcatcat caccgtgtat aaaccgctcc caattcgcca gtagctcacg
     1261 aatagagtct tcacccaccg gcggcgtata taccacgtta ttattctgat cacgcaatac
     1321 cgtgcccgga gttttcctga tatcagtttg aactgcacgt aatttggtac agatagctac
     1381 cgctgtgttt gtacagagtg gatagtcctc aagatgggta tagccatcat agagcgctgt
     1441 acgatagcgc agcgcttctt tagtggccgg atctgcgcca tctgcgcggt cagcatactg
     1501 aaagagttga tcgctggtag ttacgatatt ctctatgcgc gatgagtcct tcgcttccag
     1561 catcgggagg atgtttatca gcaacccttg gtcagggatc aactcaccag cagttttcaa
     1621 ctcagcgatt gctgcgcgcg cacttatgca tgctttaagt acgctgcgtg tttcaatgcg
     1681 ctccaggtca ggcggcagta tcggcaaatc gttgtaaggg gtttctgcat tccagttcat
     1741 atgtttatga aaaccgtttt aatcgacata taaccataat atgtcgttgt catcgacatg
     1801 tcacgtcatc atgtttaaaa aatcacattt tatcgacata tcactggaac atgccgatgc
     1861 ggtaaataca tccagacaac atgttttaaa aaagcaaatt tcatcgacac atagcaagaa
     1921 catgttgata ccttaagcat atctcgacaa tatgtttaaa aaagcgaatt ctatcgacac
     1981 atagccagaa agtgtcgatg ccataagcat at
//
LOCUS       DAHHTG010000275         2003 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.283, whole genome shotgun sequence.
ACCESSION   DAHHTG010000275 DAHHTG010000000
VERSION     DAHHTG010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2003)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2003)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2003
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.283"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            <1..490
                     /locus_tag="NBI74_004626"
     CDS             <1..490
                     /locus_tag="NBI74_004626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000643690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HCD1934830.1"
                     /translation="LKHTSYSIDEAQELLENLEYQDAPISEKDPCNIHMIPTERMEER
                     FRATKLNGSRSDAGKEFELKCHSNGYAGHKDVYGRIMIHLPANTITTGCNNPSKGRFI
                     HPWENHGITLRHAARLQTFPDDYIFWGNATEQARQIGNAVPPMLGTILINALLNIIAP
                     NR"
     gene            584..>2003
                     /locus_tag="NBI74_004627"
     CDS             584..>2003
                     /locus_tag="NBI74_004627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000236931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HCD1934831.1"
                     /translation="MVISAAFQTRARTIDHLGREQIADCPTAISELWKNAYDAYARNV
                     SLNIFDGNTPVATLVDDGHGMSLDDIINKWLTVGTESKATKKDIPYEDRNGIDHIRAK
                     QGQKGIGRLSCAALGSLMLLVSKKKDSPLVACLLDWRIFENPYLMLNDIKIPIMECSD
                     NNELITVIPEMFDALMGNLWGDGDDILRDNRIEQAWENYSELERNENNYITKEAIENT
                     VINAFFEERHFQSWPVWNNKTTHGTAMFIAGIHDDLIAQLSTDAGSEAQGAEVRAKER
                     FLQTLNSFVNPFKREGEEQITDFNTSVVAWNGNLQRFIIDEVRNFDISNFDQLEHIVE
                     GSIDESGLFSGKVKAFGEWFDNITVKPKSAYKTRKDTRFGPFFLRLGTFEVIRKNSTL
                     SDEQHATFDRIRDQFGGVMVFRDDLRVMPYGREDNDFFEIEKRRSKNAGLYMFSNRAC
                     FGGVCITKEHNPNLRDKAGREGI"
BASE COUNT          700 a          326 c          412 g          565 t
ORIGIN      
        1 tcttaaacat acttcttact caattgatga agcacaagag ctacttgaaa acctagaata
       61 tcaagacgca cccataagcg aaaaagatcc atgcaacata catatgatac caactgagcg
      121 tatggaagag cgtttcagag ccacaaaact caatggcagt agaagcgatg caggaaaaga
      181 atttgagcta aaatgtcatt ccaatggata cgcaggccat aaagatgttt atggccgcat
      241 aatgattcac ctcccagcca atacaattac aactgggtgt aacaatccat ctaagggaag
      301 attcattcat ccatgggaaa atcacggcat cactttaagg catgcggcaa ggttgcaaac
      361 gttccctgat gactatattt tttggggtaa tgcgacagag caagcaagac agattggtaa
      421 tgcagttccc cctatgttag gcacaatatt aataaatgca ttacttaaca taattgcacc
      481 caatagataa ggtgtaatgt atgaaaaata tcaaaattag aaacttaaat ggaccaaaaa
      541 atcatttgat gattacttac cttataataa tagaaggtga aaaatggtaa tttcagcagc
      601 ttttcaaaca agagcaagga caattgatca tctagggcgt gagcaaatag ctgattgtcc
      661 aaccgcaatt tccgagcttt ggaaaaatgc atatgatgct tatgctcgta atgtttctct
      721 aaatatattt gacggcaata cacctgtggc aactttagtt gatgatgggc atggcatgtc
      781 gttagatgac attatcaata agtggcttac agtaggaact gaatccaagg ctacaaaaaa
      841 agatattcca tatgaagata gaaacggaat agatcatatt cgagcaaagc aaggtcagaa
      901 aggcatcggt cgtctttctt gtgcggcctt gggctcatta atgcttttag tttccaaaaa
      961 gaaagatagc cctcttgtag cttgcctgct cgattggcgt atatttgaaa acccatattt
     1021 gatgcttaat gatataaaga tacccattat ggaatgcagt gataacaatg aattaatcac
     1081 tgttataccg gaaatgtttg atgctttgat gggaaatcta tggggtgatg gtgatgatat
     1141 attacgagat aaccgtattg aacaagcttg ggaaaattat tctgaattag aaagaaatga
     1201 aaataattat attacaaaag aagctatcga gaatactgta attaatgctt tttttgagga
     1261 aaggcatttt caatcttggc ctgtgtggaa taataaaacc actcacggca cagccatgtt
     1321 tatagctgga attcatgacg atttaatagc tcagctatca acagatgctg gttcagaagc
     1381 tcaaggtgca gaggttcggg ctaaagaacg ctttcttcaa acattaaata gctttgttaa
     1441 tccatttaaa agagaaggcg aagaacagat tactgatttc aatacaagtg ttgtcgcatg
     1501 gaatggtaat ctgcaacgat ttatcatcga tgaagttaga aactttgata tttcaaactt
     1561 tgaccagcta gaacatatag ttgaaggaag tattgatgaa agtggattat tttccgggaa
     1621 agtgaaagcc ttcggagaat ggtttgataa tattacagtc aaacctaaat ctgcatataa
     1681 gaccagaaaa gatactcgct ttggcccttt ctttttaaga ttaggcacat ttgaagttat
     1741 aagaaaaaat agtacattat cagatgaaca gcatgcaacc ttcgaccgta tccgtgatca
     1801 gtttggtgga gtaatggttt ttcgtgatga tttacgtgtt atgccatacg gacgtgaaga
     1861 taatgacttt tttgaaatcg aaaaaagacg ttcaaaaaat gctggtttat atatgttcag
     1921 taatagggca tgttttggtg gtgtatgtat aacgaaagaa cataacccca acctacgaga
     1981 taaagcaggt agagaaggta taa
//
LOCUS       DAHHTG010000276         2002 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.284, whole genome shotgun sequence.
ACCESSION   DAHHTG010000276 DAHHTG010000000
VERSION     DAHHTG010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2002)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2002)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2002
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.284"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            <1..632
                     /locus_tag="NBI74_004628"
     CDS             <1..632
                     /locus_tag="NBI74_004628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HCD1934832.1"
                     /translation="YRIMRQNALLLERKTAVPPSKRAHTGRVAVKESNQRWCSDGFEF
                     RCDNGEKLRVTFALDCCDREALHWAVTTGGFNSETVQDVMLGAVERRFGNELPASPVE
                     WLTDNGSCYRANETRQFARMLGLEPKNTAVRSPESNGIAESFVKTIKRDYISIIPKPD
                     GLTAAKNLAEAFEHYNEWHPHSALGYRSPREYLRQRACNGLSDNRCLEI"
     gene            complement(745..1938)
                     /locus_tag="NBI74_004629"
     CDS             complement(745..1938)
                     /locus_tag="NBI74_004629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCD1934833.1"
                     /translation="MSPGIEDTPQKPLSCWPLAFSAGLLGIGQNGLLVVLPVLVIQTN
                     LSLSVWAALLMLGSMLFLPSSPCWGKQISRTGSKPVVLWALGGYGISFTLLGLGSVLM
                     ATSAITTAVGLGILIIARIAYGLTVSAMVPACQVWALQRAGEGNRMAALATISSGLSC
                     GRLFGPLCAAAMLAIHPLAPLGLLMAAPVLALLMLLRLPGTPPQPTPECKSVSLKRDC
                     LPYLLCAILLAAAVSMMQLGLSPALTRQFATDTTAISQQVAWLLGLSAVAALIAQFGV
                     LRPQRLTPVALLLSAGVLMSGGLAIMLSEQLWLFYPGCAVLSFGAALATPAYQLLLND
                     KLADGAGAGWLATSHTLGYGLCALLVPLVSKTGVAIALIMAALFATILFTIVSVFIWH
                     YRTIK"
BASE COUNT          512 a          535 c          558 g          397 t
ORIGIN      
        1 tttaccggat catgcgccag aatgcgctgt tgcttgagcg aaaaaccgct gtaccgccat
       61 cgaaacgggc acataccggc agagtggctg tgaaagaaag taatcagcga tggtgctctg
      121 acgggttcga gttccgctgt gataacggag aaaaactgcg ggtcacgttc gcgctggact
      181 gctgtgaccg tgaggcactg cactgggcgg tcactacggg cggcttcaac agtgaaacag
      241 tacaggacgt catgctggga gcggtggaac gccgcttcgg caacgagctt ccggcgtctc
      301 cagtagagtg gctgacggat aatggttcat gctaccgggc taatgaaaca cggcagtttg
      361 cccggatgtt ggggcttgaa ccgaaaaaca cggcggtgcg gagtccggag agtaacggca
      421 tagcagagag cttcgtgaaa acgataaagc gtgactacat aagtatcata cccaaaccag
      481 acgggttaac ggcagcaaag aaccttgcag aggcgttcga gcattataac gaatggcatc
      541 cgcatagtgc gttgggttat cgctcgccac gggaatatct gcggcagcgg gcctgtaatg
      601 ggttaagtga taacagatgt ctggaaatat aggggcaaat ccaggcgggg gcagcaagcc
      661 agaacactgc ctgacatgtt ttcatcagac gcaggtcctc tctcatatcc tctgatttac
      721 tccagtaagg tgatgatcat atctttattt gatagtacgg taatgccaga taaatacaga
      781 cacaatggta aataatatag ttgcaaataa tgctgccata atcagcgcga ttgcgacacc
      841 tgttttcgat accagcggca ccagcaaagc gcacagtcca tagccaagtg tgtgactggt
      901 agcgagccag cccgcgcctg cgccatcagc cagcttatca ttcagtagaa gttgataagc
      961 gggtgttgcc agagctgctc caaatgacag cactgcacag cccgggtaaa acaaccatag
     1021 ctgttcggag agcatgatag ccagaccacc actcatcaac actccggcac tcaggagcag
     1081 ggccaccgga gtcaggcgct gcggacggag taccccgaac tgcgcgataa gcgcagctac
     1141 tgcggacaga cccaacagcc aggccacctg ttggctaatg gcggtggtat ccgtggcgaa
     1201 ctggcgagta agggcaggcg aaagtccaag ctgcatcatg ctcaccgccg cagccagtaa
     1261 tattgcgcaa agcagataag gcagacaatc ccgcttcaga ctgacgctct tgcactccgg
     1321 tgtgggctgt ggtggtgtgc cgggcaaccg cagcagcatc agcagcgcca gcactggggc
     1381 tgccatcagc agccccagtg gcgccagagg atgaatggcc agcattgctg ccgcgcacag
     1441 cggcccgaat agcctgccgc aactcaggcc ggagctgatg gttgccagag cggccatgcg
     1501 attcccttct ccagctctct gcaatgccca gacctgacag gctggcacca ttgccgacac
     1561 ggtcagcccg taggcgatcc gggcgatgat taatattccc agtcccaccg ctgttgttat
     1621 ggcgctggta gccatcagca cgcttcccag ccctagcagg gtaaagctta tgccatatcc
     1681 tcccagagcc cacaacacca caggtttact gccagtacgg gaaatttgct ttccccagca
     1741 cggggacgat ggcagaaaca gcattgagcc aagcatcagc agggcagccc atacagacag
     1801 actcagattt gtctgtatga ccagaacagg gagcacaacc agcagaccgt tctgtccgat
     1861 accgagaagc ccggcactga acgcaagtgg ccagcaggac agtggttttt ggggcgtatc
     1921 ttcgatccct ggtgacatta taatgtttca actccatgta ttaattgtgt ttatttgtaa
     1981 aattaattta tctgacaata ac
//
LOCUS       DAHHTG010000277         1974 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.285, whole genome shotgun sequence.
ACCESSION   DAHHTG010000277 DAHHTG010000000
VERSION     DAHHTG010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1974)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1974)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1974
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.285"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            complement(51..1457)
                     /gene="gndA"
                     /locus_tag="NBI74_004630"
     CDS             complement(51..1457)
                     /gene="gndA"
                     /locus_tag="NBI74_004630"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HCD1934834.1"
                     /translation="MSKQQIGVVGMAVMGRNLALNIESRGYTVSIFNRSREKTEEVIA
                     ENPGKKLVPYYTVKEFVESLETPRRILLMVKAGAGTDAAIDSLKPYLDKGDIIIDGGN
                     TFFQDTIRRNRELSAEGFNFIGTGVSGGEEGALKGPSIMPGGQKEAYELVAPILTKIA
                     AVAEDGEPCVTYIGADGAGHYVKMVHNGIEYGDMQLIAEAYSLLKGGLNLTNEELAQT
                     FTEWNNGELSSYLIDITKDIFTKKDEDGNYLVDVILDEAANKGTGKWTSQSALDLGEP
                     LSLITESVFARYISSLKDQRVAASKVLSGPQAQPAGDKAEFIEKVRRALYLGKIVSYA
                     QGFSQLRAASEEYNWDLNYGEIAKIFRAGCIIRAQFLQKITDAYAENPQIANLLLAPY
                     FKQIADDYQQALRDVVAYAVQNGIPVPTFSAAVAYYDSYRAAVLPANLIQAQRDYFGA
                     HTYKRIDKEGVFHTEWLD"
     gene            complement(1585..>1974)
                     /locus_tag="NBI74_004631"
     CDS             complement(1585..>1974)
                     /locus_tag="NBI74_004631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HCD1934835.1"
                     /translation="VLSDFIVSFMLGINKTKIPKESIYSDTVVDWCAGSFMLVRFSDF
                     VRVNGFDQGYFMYCEDIDLCLRLSLAGVRLHYVPAFHAIHYAHHDNRSFFSKAFRWHL
                     KSTFRYLARKRILSNRNFDRISSVFHP"
BASE COUNT          544 a          476 c          442 g          512 t
ORIGIN      
        1 gcccggtgca atatacgccg ggcctcaatt ttattgttgg ttaaatcaga ttaatccagc
       61 cattcggtat ggaacacacc ttctttatca atacgcttat aagtatgcgc accaaaatag
      121 tcacgctgtg cctggatcag gttcgcaggc agaacagcag cacggtagct gtcgtaatag
      181 gcaaccgctg cggagaaggt cggaaccgga ataccgttct gtactgcata agcaacgaca
      241 tcacgcagcg cctgctggta gtcatcggca atttgcttga agtacggagc cagcaacagg
      301 ttagcgatct gtggattttc ggcataagca tcggtgattt tctgcaggaa ctgcgcacgg
      361 atgatgcagc cagcacggaa aatcttcgcg atttcgccgt agttcagatc ccagttgtac
      421 tcttcagacg cagcacgcag ctgagagaag ccctgggcgt aagaaacgat tttgcccaga
      481 tacagcgcac gacgaacttt ttcgatgaac tcagccttgt cgcctgctgg ctgtgcttgc
      541 ggaccagaga gaactttaga tgcggcaaca cgctgatctt tcagagaaga gatataacgt
      601 gcaaacacag actcggtaat cagcgacagc ggttcgccga gatccagcgc gctctggctg
      661 gtccatttac cggtaccttt gttagccgct tcatccagga tcacatcaac caggtagtta
      721 ccgtcttcat cttttttggt gaagatatct ttggtgatgt cgatcaggta actgctcagt
      781 tcaccgttat tccactcggt aaaggtctgc gccagttctt cgttggtgag gttcaggcca
      841 cctttaagca gagaataggc ttcagcaatc agctgcatat cgccgtattc aataccgttg
      901 tgaaccatct tcacatagtg acctgcgcca tcggcaccaa tataggtaac gcatggttca
      961 ccgtcttcag ctacggcggc gattttggtc aggatcggtg ctaccaattc ataggcttct
     1021 ttctggccac caggcataat agaaggacct ttcagcgccc cctcttcacc gccagaaaca
     1081 ccggtaccga tgaagttaaa gccctctgct gaaagctcac gattacgacg aatagtgtcc
     1141 tggaagaagg tgttaccacc atcaatgatg atgtctcctt tatcgagata tggtttgagg
     1201 gaatcaatag cagcatccgt gcctgcacct gctttcacca ttaacaggat gcgacgaggc
     1261 gtttccagag attcgacaaa ctctttcacc gtatagtaag gaaccagttt cttgcctgga
     1321 ttttcggcaa tcacttcttc cgtcttctca cgggaacggt tgaaaataga gacggtataa
     1381 ccacggcttt cgatgttgag cgcaaggttg cgtcccatca ctgccatacc gactacgccg
     1441 atctgttgct tggacattac atactcctgt caggtgtgat caccgcgctg aatgctcgcg
     1501 gcctggaatg ttcgcaaata agtatacaaa gtacttatta aagtataaat agcttatcca
     1561 tgcttatatg cttacggctt tatattacgg gtgaaaaact gatgaaattc gatcaaagtt
     1621 gcgatttgat aaaatacgtt ttctggctaa atatctaaaa gtacttttta agtgccatct
     1681 gaaggctttt gaaaaaaaac ttcgattgtc atgatgagca taatgtatcg catgaaaagc
     1741 gggaacataa tgaagtctga caccagccag gctaagcctc aagcacaggt caatatcttc
     1801 acagtacata aagtaacctt gatcgaagcc atttacacgc acaaaatctg aaaaacgtac
     1861 cagcataaat gatcctgcgc accaatcaac aaccgtatca gaatagatac tttctttagg
     1921 aatttttgtt ttattaatcc ctaacataaa tgacacaata aaatcagaaa gcac
//
LOCUS       DAHHTG010000278         1870 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.286, whole genome shotgun sequence.
ACCESSION   DAHHTG010000278 DAHHTG010000000
VERSION     DAHHTG010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1870)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1870)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1870
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.286"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            361..576
                     /gene="essD"
                     /locus_tag="NBI74_004632"
     CDS             361..576
                     /gene="essD"
                     /locus_tag="NBI74_004632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416074.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage lysis protein EssD"
                     /protein_id="HCD1934836.1"
                     /translation="MKSMDKLTTGVAYGTSAGNAGFWALQLLDKVTPSQWAAIGVLGS
                     LVFGLLTYLTNLYFKIKEDRRKAARGE"
     gene            581..892
                     /locus_tag="NBI74_004633"
     CDS             581..892
                     /locus_tag="NBI74_004633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdfR family protein"
                     /protein_id="HCD1934837.1"
                     /translation="MTQDYELVVKGVRNFENKVTVTVALQDKERFDGEIFDLDVAMDR
                     VEGAALEFYEAAARRSVRQVFLEVAEKLSEKVESYLQHQYSFKIENPANKHERPHHKY
                     I"
     gene            889..1422
                     /locus_tag="NBI74_004634"
     CDS             889..1422
                     /locus_tag="NBI74_004634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HCD1934838.1"
                     /translation="MNTKIRYGLSAAVLALIGAGASAPQILDQFLDEKEGNHTMAYRD
                     GSGIWTICRGATVVDGKTVFPNMKLSKEKCDQVNAIERDKALAWVERNIKVPLTEPQK
                     AGIASFCPYNIGPGKCFPSTFYKRLNAGDRKGACEAIRWWIKDGGRDCRIRSNNCYGQ
                     VIRRDQESALTCWGIEQ"
     gene            1419..>1870
                     /locus_tag="NBI74_004635"
     CDS             1419..>1870
                     /locus_tag="NBI74_004635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2514 domain-containing protein"
                     /protein_id="HCD1934839.1"
                     /translation="MNQIFMVIFLVLSGFIVGNVWSDRGWQKKWAERDAAALSQEVNA
                     QFAARIIEQGRTIARDEAVKDAQQKSAEISARAAYLSDSVNQLRAEAKKYAIRLDAAK
                     HTADLAAAVRGKTTKTAEGMLTNMLGDIAAEAQLYAEIADERYIAGVT"
BASE COUNT          524 a          378 c          475 g          493 t
ORIGIN      
        1 actttgttgc aatgagggca gatcaaaagg gcaccttttt gtactcttga aaaactgtgt
       61 tctgactctt gggtgcagtt tgggcaggaa catttaacga gataattacg gcgtgatttt
      121 gagtctttac gttctgacat aggcttttcc tgtataaatg gccgtataca gtacactaaa
      181 tatgaaaaca tttctcgtat tattatttta tatatgactt tctttcaaaa taattactca
      241 catttttaat gtgtatgttt ctttagcgcc gttgagaaca acgtgtgctg tcaaaactac
      301 cccgtagact ccgatctttt tcaaacatat tgcaccatcc gtgtacatcg gggtgaggat
      361 atgaaatcaa tggataagtt aacaacaggt gttgcctatg gcacatcggc gggtaatgct
      421 ggtttctggg cattacagtt actcgataaa gtaactccgt cacagtgggc tgcaatcggt
      481 gtgctgggta gcctggtttt tggcctgctg acgtatctga caaatcttta tttcaagatt
      541 aaagaagaca ggcgtaaggc tgcgagagga gagtaatcca atgactcaag actatgaact
      601 ggttgtgaaa ggagtccgta attttgagaa taaagttacg gtaactgtag ccttacagga
      661 caaggaacgc tttgacggtg aaatttttga cctggatgtc gccatggacc gtgttgaagg
      721 agctgcgctg gagttttatg aggcagcagc cagaaggagc gtccggcaag tcttcctgga
      781 agtagcagaa aaattgtcag aaaaagttga gtcttatctg cagcatcagt actcctttaa
      841 gattgaaaat cctgccaata agcacgagcg tcctcatcat aaatatatat gaacacaaaa
      901 atcagatacg gcctgtcggc tgccgttctg gcgctgattg gtgctggcgc atctgctcct
      961 cagatacttg accagtttct ggacgaaaaa gaaggtaacc acacaatggc ataccgcgat
     1021 ggttctggca tatggaccat ctgtcggggt gccacagtgg tggatggaaa aaccgttttt
     1081 cccaatatga aactgtcgaa ggaaaaatgc gaccaggtca acgccattga gcgtgataag
     1141 gcgctggcat gggtggagcg caatattaaa gtaccactga ccgaaccaca aaaagcgggt
     1201 atcgcgtcat tttgtcccta taacattggc cccggtaagt gtttcccgtc gacgttttat
     1261 aagcggctga atgctggtga tcgtaaaggt gcatgcgaag cgattcgctg gtggattaag
     1321 gatggcggac gcgattgccg cattcgttca aataactgtt acggtcaggt tattcgtcgt
     1381 gaccaggaga gcgcattaac ctgctggggg atagaacagt gaatcagata ttcatggtga
     1441 tttttctcgt gttgtcagga tttatcgtcg gaaatgtctg gagcgacaga ggatggcaaa
     1501 aaaaatgggc ggaacgtgat gctgccgcat tatcacaaga ggtaaatgct caatttgctg
     1561 ctcgaataat tgaacagggg cgaactatag cccgtgatga ggctgttaaa gatgcacaac
     1621 agaaatctgc tgaaatttct gccagggctg cttatctgtc tgatagtgtt aaccagttgc
     1681 gtgccgaagc aaaaaaatat gccatacgcc ttgacgcagc gaagcatacc gcagatcttg
     1741 ccgctgccgt cagaggcaaa acaaccaaaa ccgccgaagg aatgctcacc aacatgctcg
     1801 gagatattgc agcagaagct cagctttatg ctgaaattgc tgacgaacgc tacatcgcag
     1861 gagtgacttg
//
LOCUS       DAHHTG010000279         1856 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.287, whole genome shotgun sequence.
ACCESSION   DAHHTG010000279 DAHHTG010000000
VERSION     DAHHTG010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1856)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1856)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1856
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.287"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            complement(1..475)
                     /locus_tag="NBI74_004636"
                     /pseudo
     CDS             complement(1..475)
                     /locus_tag="NBI74_004636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611997.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS600 family transposase"
     gene            complement(488..1096)
                     /locus_tag="NBI74_004637"
                     /pseudo
     CDS             complement(488..1096)
                     /locus_tag="NBI74_004637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004389801.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30-like element IS30 family transposase"
     gene            1168..1856
                     /locus_tag="NBI74_004638"
                     /pseudo
     CDS             1168..1856
                     /locus_tag="NBI74_004638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611483.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEc52 family transposase"
BASE COUNT          451 a          452 c          458 g          495 t
ORIGIN      
        1 catctttgat gccagcgagg tacaaccatc cctcctgtgt ggcaacatac gtcaggtccg
       61 ccacccagac ctgatttggt gctgtaggag cgaacgtctg gttcagcaga tttggcgcaa
      121 ctggcagatt gtggttcgag ttcgtagtcg ctctgaactt gcgtttctgc ttacagcgta
      181 gccttagctc cttacgaaga cgtgccagtc ggtcacgacc aacgatgatg ccattctctg
      241 ccagctccgt ctggagccgc cgggttccat atgtttcgcg agtgcggata tgtgccacct
      301 taatctccag ttttagccgc tcatcacttt gttttctgtc tgagggttca tgctgtaccc
      361 agttgtaata accgctcctg gatacaccaa atacctgaca catcgcttca atgggaaatt
      421 gttgtcgcca ttgttcgatt aacgcgtatt tttcagcgac tcctgtgcaa aatacgcggt
      481 tgtagattca atctgtcaat gcaacacccc tttcaattat ctctttcggt gttttgaact
      541 tcagtgtctt tctcggtctg ttgtttagct gagcagcaac cagatctagt tcatgttgag
      601 tatattgggc aagacatgtc tttttaggaa agtactgccg aattagccca tttgtgttct
      661 catttgttcc ccgctgccaa ggactctgag gatcgcagaa gtaaacttta acgccggtgc
      721 tgacagtaaa ttctagatgt ctggccagtt ccattcctct gtcccatgtc agtgattttc
      781 tgagttctga cggtaaactc aggaatttgt cggtaagagc ctgatttact gagacagaat
      841 ctttgcccct gagtctaagg atgatcgtat aacgtgattt tcggtctaca agtgtggcta
      901 tatgagagtt ttttgtacct gagactaaat cgccctccca atgtcccaga gagcgtctgt
      961 tatcgatatt tcgggaacgt tcgtgaattg gtgttccgtt cactatgtta atcgtacctc
     1021 tttcgccttt gcgggtatga cgcctgccat ggcgaaggct atgcgaccgt cgcagatgct
     1081 gtatattcag gtggttgaag tggtttactg aatttggcca cctgaacaga ggtgatatgc
     1141 tcacctcaga acaacacagg tgccataatg aaaaaaagaa atttcagcgc agagtttaaa
     1201 cgcgaatccg ctcaactggt cgttgaccag aattacaccg tggcagatgc agccagcgct
     1261 atggatgtcg gcctttccac aatgacgcga tgggtgaaac aattacgtga tgagcggcag
     1321 ggaaaaacac caaaagcctc ccctattacc ccggaacaaa ttgaaatccg tgagctcagg
     1381 aaaaagctac aacgtattga aatggaaaat gaaatattaa aaaaggctac cgcgctcttg
     1441 atgtcagact ccctgaacag ttctcgataa tcgggaaact cagggcgcgt tatcctgtgg
     1501 ccactctctg ccatgtgttc ggggtccatc gcagcagcta caaatactgg aaaaaccgtc
     1561 ctgaaaagcc agacggcaga cgggctgtat tacgcagcca ggtacttgaa ctgcatggca
     1621 tcagccacgg ctctgccgga gcaagaagca tcgccacaat ggcaacccag agaggctacc
     1681 agatggggcg ctggcttgct ggcagactca tgaaagagct ggggctggtc agctgtcagc
     1741 agccgactta ccggtataaa cgtggtggtc atgaacatgt tgctatccct aactaccttg
     1801 aacggcagtt cgccgtgacc gagccaaatc aggtgtggtg cggtgatgtg acctat
//
LOCUS       DAHHTG010000280         1819 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.288, whole genome shotgun sequence.
ACCESSION   DAHHTG010000280 DAHHTG010000000
VERSION     DAHHTG010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1819)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1819)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1819
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.288"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            complement(133..1545)
                     /gene="pykF"
                     /locus_tag="NBI74_004639"
     CDS             complement(133..1545)
                     /gene="pykF"
                     /locus_tag="NBI74_004639"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010346010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase PykF"
                     /protein_id="HCD1934840.1"
                     /translation="MKKTKIVCTIGPKTESEEMLAKMLDAGMNVMRLNFSHGDYAEHG
                     QRIQNLRNVMSKTGKTAAILLDTKGPEIRTMKLEGGNDVSLKAGQTFTFTTDKSVIGN
                     SEMVAVTYEGFTTDLSVGNTVLVDDGLIGMEVTAIEGNKVICKVLNNGDLGENKGVNL
                     PGVSIALPALAEKDKQDLIFGCEQGVDFVAASFIRKRSDVIEIREHLKAHGGENIHII
                     SKIENQEGLNNFDEILEASDGIMVARGDLGVEIPVEEVIFAQKMMIEKCIRARKVVIT
                     ATQMLDSMIKNPRPTRAEAGDVANAILDGTDAVMLSGESAKGKYPLEAVSIMATICER
                     TDRVMNSRLEFNNDNRKLRITEAVCRGAVETAEKLDAPLIVVATQGGKSARAVRKYFP
                     DATILALTTNEKTAHQLVLSKGVVPQLVKEITSTDDFYRLGKELALQSGLAHKGDVVV
                     MVSGALVPSGTTNTASVHVL"
BASE COUNT          446 a          426 c          448 g          499 t
ORIGIN      
        1 ggattcatct tagataaagt gagattatat agtgaaatcc gatttgattt tttattgctt
       61 ctggttatcg attaaataaa aaaagcgccc atcaggacgc ttcgatatac aaattaattc
      121 acaaaagcaa tattacagga cgtgaacaga tgcggtgtta gtagtgccgc tcggtaccag
      181 tgcaccagaa accataacta cgacatcacc tttgtgtgcc agaccgctct gcagagccag
      241 ttctttaccc agacggtaga agtcatcagt agaagtgatc tctttaacca gctgcggcac
      301 aacgcctttg ctcagtacta actggtgagc cgttttttcg ttggtggtca gtgccaggat
      361 ggtggcatcc gggaagtatt tacgtactgc gcgagcagat ttaccgccct gagtagcaac
      421 cacgatcagc ggagcatcca gtttttcagc agtttcaacg gcaccacggc ataccgcttc
      481 ggttatacgc agtttacggt tgtcattgtt gaactcgaga cggctgttca tcacgcggtc
      541 ggtacgttcg cagatggtcg ccatgataga aaccgcttcc agcgggtatt taccttttgc
      601 ggattcacca gacagcatca ctgcgtcagt accgtcgagg atggcgtttg caacgtcacc
      661 ggcttctgcg cgagtcgggc gtgggttttt gatcatggaa tccagcatct gggtcgcagt
      721 gataacgact ttacgtgcac ggatacattt ttcgatcatc atcttctggg cgaagataac
      781 ttcttctacc gggatttcta cacccaggtc gccacgcgca accatgatgc cgtcagaggc
      841 ttcgaggatt tcgtcgaagt tgttgaggcc ttcctggttt tcgattttgg agatgatgtg
      901 gatgttttcg ccgccgtgcg ctttcaggtg ctcacggatt tcgataacgt cagaacgctt
      961 acgaataaag gaagcagcaa caaagtctac gccttgttcg caaccaaaga tcaggtcctg
     1021 tttgtctttt tcagccagtg ctggcagggc aatggaaacg ccaggcaggt tcacaccttt
     1081 gttttcgccc aggtcgccgt tgttcagcac tttacagata actttgttac cttcaatggc
     1141 ggtaacttcc ataccgatca gaccgtcgtc aaccagtacg gtgttgccaa cagacaggtc
     1201 agtagtgaaa ccttcatacg ttaccgcaac catttcgctg ttgccgataa cagatttatc
     1261 agtagtgaaa gtaaaggtct gacccgcttt cagagaaacg tcgttaccgc cttccagttt
     1321 catggtgcgg atttccggac ctttggtatc aagcaggata gcggcggttt taccagtttt
     1381 gctcatcacg ttgcgcagat tctgaatgcg ctgaccgtgt tctgcatagt caccatgaga
     1441 gaagttcaga cgcataacgt tcatgccagc gtccagcatt ttagctaaca tctcttcaga
     1501 ttcggttttc ggtccgatgg tgcaaacaat tttggtcttt ttcatgacag tcttagtctt
     1561 taagttgaga aggatgggag aaacttgctt tctgggcgca ctgccggaaa gcgaatccgg
     1621 tattacgaaa gtggttgtgc ctcgctctaa ggataggtga catcgaaaaa gcgtgcagag
     1681 gaatgtgtgc ttgtgtctca gcccaacgtg agtgcctgtt tttgagtcgt tgagttctac
     1741 agtccaaagt gctgaaacca ttcaagagtc aattggcgcg cattatacgc tgaaattaat
     1801 ccaaaaggaa aacaaaaac
//
LOCUS       DAHHTG010000281         1780 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.289, whole genome shotgun sequence.
ACCESSION   DAHHTG010000281 DAHHTG010000000
VERSION     DAHHTG010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1780)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1780)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1780
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.289"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            <1..1490
                     /locus_tag="NBI74_004640"
     CDS             <1..1490
                     /locus_tag="NBI74_004640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000236931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HCD1934841.1"
                     /translation="KRFIGRASNIRDEKLEEINAKHAALKADEDRKKLLRKEQRRIKT
                     SIQRDRISLEHLRNEFYEISQLLSDKNNFKELEELLQLKENIDVLDGTLKNLSLGSVP
                     RNLGSIEKDYRQYRDLEIDAKSLLKQINNSVYSALDHFTVKDDYSIAEKDFRSKAAIL
                     HAKIRKFSNKGRNILKEEMLRFEKITNNTNKAFHEKTSQYLSDLQENRTSLKKTLENL
                     DLAYQIQDIEIGQTYAPYITALESLREEIDLEGLAISSVNENTRLKKQVEQVNALAQL
                     GITVEIIGHEIEGFDMTIERGINRLSSTNLDEYQKNALSSITQAHQSLSDSWRFLSPL
                     KLSGDKVRAFLSGKDIFDYVNHFFNSKFEKDSIEFSCSTNFLDISLYDQPARIYPVFI
                     NLVNNSRYWVKETKEERRIIRLDVLDGLIYVSDNGPGVDPDDVSELFTIFFSKKQRGG
                     RGVGLYLCKQNLAVSGHSIFYETRTEKKILNGANFVINFKGIKNA"
     gene            1483..>1780
                     /locus_tag="NBI74_004641"
     CDS             1483..>1780
                     /locus_tag="NBI74_004641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000886596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1934842.1"
                     /translation="MLDNSTFDYKPHLKSAYIDPIRTVTVIDDEYPTIDDLISPTKDS
                     FSQDNISRLKDIIDISRSEEYNWLLDVYNGKEKKIQEGTVSNRLYHSDLLILDYH"
BASE COUNT          669 a          273 c          315 g          523 t
ORIGIN      
        1 caaaaaggtt tattggccgc gcatcaaata tacgagatga aaagctagag gaaataaatg
       61 ctaaacatgc tgctttgaaa gcagacgaag atagaaaaaa attattacgt aaagagcaaa
      121 gaagaatcaa aacatcgatt caaagagatc gtatttcttt agaacattta agaaatgaat
      181 tttatgaaat atcacagctt ctaagcgaca agaataattt taaagaacta gaggagctat
      241 tacagctcaa agaaaacatc gacgtattgg atggtaccct aaaaaaccta tctttaggtt
      301 cagtaccaag aaatttaggg agtatagaga aagactaccg tcagtatcgc gatttagaga
      361 ttgatgctaa aagtctttta aagcagatta ataactctgt atactcagcg cttgatcatt
      421 ttactgttaa agatgattat tcaattgctg agaaagactt tcgtagcaaa gcagccatat
      481 tacatgcgaa aataagaaaa ttttccaata aaggacgcaa tatattaaaa gaagagatgt
      541 tgcgtttcga aaagataaca aacaatacaa ataaagcttt ccatgaaaaa acatctcaat
      601 atttatccga tctacaagaa aatagaactt cactcaaaaa aacacttgaa aatttagatc
      661 ttgcttatca gattcaagac attgaaatag gtcaaaccta cgccccatat attaccgcat
      721 tagaaagctt aagagaggaa attgatttag aaggcctcgc gatctcttca gtcaacgaaa
      781 atacacggtt gaagaaacag gtagagcaag tgaatgcact cgctcaactt ggaataactg
      841 tggagataat tggtcatgaa atcgaaggtt tcgatatgac tattgagcga ggtataaata
      901 gactgtcatc aacaaacctc gatgaatatc agaaaaatgc tttatcaagt attacccaag
      961 cacatcaatc attaagcgat tcttggcgtt ttttaagccc attaaaatta tcaggagata
     1021 aggtaagagc tttcttgagt ggaaaagata tttttgatta tgttaatcat tttttcaaca
     1081 gtaaatttga aaaagattca attgaatttt cttgctctac taatttccta gatatttcat
     1141 tatatgatca accagccaga atttatcctg tgtttattaa tttagtaaac aactcacgat
     1201 attgggttaa agaaactaaa gaagagcgtc gaattattag gttagatgta cttgatggtt
     1261 tgatatatgt tagtgataat gggccagggg ttgatcctga tgacgtgtcc gaacttttca
     1321 ctatattttt ctccaagaaa caaagaggtg gtcgcggggt tggcctttat ctctgcaaac
     1381 aaaatttagc ggtgagtggc catagtattt tctacgaaac aagaacagag aaaaaaatac
     1441 taaatggtgc taattttgta attaatttca aaggaattaa aaatgcttga taattctact
     1501 ttcgattaca aaccacattt aaaatctgct tatattgatc cgattagaac tgtgacagtc
     1561 atcgatgatg aatacccaac tattgatgat ttaatttcac cgaccaaaga cagtttttct
     1621 caagacaaca tttctcgatt aaaagatatt attgatataa gtcgaagtga agaatataat
     1681 tggcttttag atgtctataa tggaaaagag aagaaaattc aagagggaac cgtatctaac
     1741 cgtctttatc acagtgatct actaatcttg gactatcatt
//
LOCUS       DAHHTG010000282         1695 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.290, whole genome shotgun sequence.
ACCESSION   DAHHTG010000282 DAHHTG010000000
VERSION     DAHHTG010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1695)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1695)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1695
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.290"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            complement(<1..1620)
                     /gene="hcp"
                     /locus_tag="NBI74_004642"
     CDS             complement(<1..1620)
                     /gene="hcp"
                     /locus_tag="NBI74_004642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tube protein Hcp"
                     /protein_id="HCD1934843.1"
                     /translation="MGDIVYLRIIGEKQGDISSGCGTYASVGNRWQVGHEDEIFAFAL
                     TNAITSTGNGVNLQGLQFCKLIDKSSPLLSNAINQNERLFIEIDLYRINKSGRWERYY
                     YIQLRNASLTAIHVNISDNNLPTECVTVDYDYILCKHLIANTEFDWLAFPAGYNSLFI
                     PPKSPPASNLKPEPLPVVNLPLSSPAVNPVYAKSCLKVKGCTDAGTAEEPAENFGQVA
                     IFVQPVVDDCCGYHHPEANVIGEPAEAQTMLLFPSSVLATQIWGKWSLSGVLSATRGS
                     YIGALASALYIPSAGEGSARVPGRDEFWYEEELRQKALAGSTATTRVRFFWGTDIHGK
                     PQVYGVHTGEGTPYENVRVANMQWNEQTQRYEFTPAHDVDGPLITWTPENPEHGNVPG
                     HTGNDRPPLEQPTILVTPIPDGTDTYTTPPFPVPDPKEFNDYILVFPAGSGIKPIYVY
                     LKEDPRKLPGVVTGHGVPLSPGTRWLDMSVSNNGNGAPIPAHIVDKLRGREFKTFDEF
                     REALWLEVSQEPELIAQFSEINQLRISQGFTP"
BASE COUNT          440 a          435 c          391 g          429 t
ORIGIN      
        1 aggtgtaaag ccctgactaa tacgtaactg attaatttcc gaaaactgcg ctatcaactc
       61 cggttcctga ctcacctcca gccacagcgc ctcgcgaaac tcatcaaagg ttttaaactc
      121 ccgtccgcgc aatttatcaa caatatgcgc cgggattggt gcgccgttgc cgttattgga
      181 taccgacata tccagccagc gagttcctgg tgaaagcggg acgccgtgcc ctgttacaac
      241 accaggcagc tttcgcggat cctccttcag gtaaacatag ataggcttaa taccggatcc
      301 cgccggaaaa accagaatat aatcgttgaa ttctttcgga tcaggaaccg ggaatggcgg
      361 cgtggtatag gtatcggtgc cgtccggaat cggcgtcacc agaatggtgg gctgctccag
      421 cggcggcctg tcgttaccgg tatggcccgg aacattccca tgttccggat tttccggcgt
      481 ccaggtaatc agggggccat cgacatcgtg agcgggggta aattcataac gctgcgtctg
      541 ctcgttccac tgcatgttcg ccacgcggac gttttcatac ggcgtacctt cacccgtatg
      601 aacaccatac acctgaggct tgccgtgaat gtcagttccc cagaaaaaac gcacccgggt
      661 ggtggcggta ctgcctgcta gtgctttctg ccgcagttct tcctcatacc agaactcatc
      721 acgtccaggc acacgagcac tgccctcacc cgcagaggga atatacagag cagatgccaa
      781 cgcaccgatg taagagccgc gggttgcact gagtacgcca ctgagcgacc attttcccca
      841 tatttgagtc gccaacacac tactcgggaa cagtagcatg gtttgagctt ctgcgggttc
      901 cccgataacg ttagcctcgg gatggtggta tccacagcag tcatcaacca ctggctgaac
      961 gaaaatcgct acttgaccga agttttcagc gggttcttct gccgttccgg catctgtaca
     1021 tcccttcacc ttcagacagg atttggcata gaccgggtta accgctggtg aagagagtgg
     1081 aaggttaaca actggtagcg gttcaggctt aagattactg gcaggtggac ttttaggcgg
     1141 aataaataag ctattatagc cagcaggaaa ggccaaccag tcaaattccg tattggctat
     1201 gagatgttta cataatatgt agtcataatc gacagttaca cattcggtag gaagattatt
     1261 gtcagaaatg tttacatgaa tagcagttaa tgaagcattt cttagctgaa tataataata
     1321 ccgttcccat cgcccgcttt tatttatacg atacaaatcg atttcaataa ataaccgctc
     1381 attctgattg atggcattag acagtagcgg tgagctttta tcaatgagtt tgcaaaattg
     1441 tagcccctgt agattaacgc cattaccagt actggtaatg gcgttggtga gtgcaaaggc
     1501 aaaaatttca tcttcatggc caacctgcca gcgattacca accgacgcat acgtcccaca
     1561 gcctgaagaa atatcgccct gcttctcacc gattattctc aggtaaacaa tatcgcccac
     1621 tttatttcat tcccgtttat ccttaactta tttgcattaa atagaaaact tatttctcga
     1681 tcaataagaa acgct
//
LOCUS       DAHHTG010000283         1655 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.291, whole genome shotgun sequence.
ACCESSION   DAHHTG010000283 DAHHTG010000000
VERSION     DAHHTG010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1655)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1655)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1655
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.291"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            complement(<1..948)
                     /gene="ag43"
                     /locus_tag="NBI74_004643"
     CDS             complement(<1..948)
                     /gene="ag43"
                     /locus_tag="NBI74_004643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter adhesin Ag43"
                     /protein_id="HCD1934844.1"
                     /translation="MKRHLNTSYRLVWNHITGTLVVASELARSRGKRTGVAVALSLAA
                     VTSLPALAADTVVQAGETVNGGTLENHDNQIVFGTANGMTISTGLEYGPDNEANTGGQ
                     WIQNGGIANNTTVTGGGLQRVNAGGSVSDTVISAGGGQSLQGQAVNTTLNGGEQWVHE
                     GGIATGTVINEKGWQAVKSGAVATDTVVNTGAEGGPDAENGDTGQFVRGNAARTTINK
                     NGRQIVAVEGTANTTVVYAGGDQTVHGHALDTTLNGGYQYVHNGGTASDTVVNSDGWQ
                     IVKEGGLADFTTVNQKGKLQVNAGGTATNVTLKQGGALVT"
     gene            complement(1320..>1655)
                     /locus_tag="NBI74_004644"
     CDS             complement(1320..>1655)
                     /locus_tag="NBI74_004644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase family protein"
                     /protein_id="HCD1934845.1"
                     /translation="EKTDAVFRLFRPVHPVVAVSACTGWELDTLVSALMTALPDHAAS
                     PLMTRLQDELRTESVRSQAREQFTGAVDRIFDTAESVCIASVARAVLRAVRDSVVSVA
                     RAVWNWIFF"
BASE COUNT          356 a          485 c          417 g          397 t
ORIGIN      
        1 ggtgaccagt gctccaccct gtttcagggt gacattcgtg gctgtaccac cggcgttcac
       61 ctgcagtttg cctttctggt taacggtggt gaaatccgcc agaccacctt ccttgacaat
      121 ctgccagccg tcactgttta caaccgtgtc agaggctgtg cctccgttgt gcacatactg
      181 gtaaccgcca ttcagtgtgg tatccagcgc atgcccgtgt accgtctggt cgccgccggc
      241 ataaaccaca gtggtatttg ctgttccttc aacagccaca atctgacgac catttttatt
      301 gatggtggta cgtgcggcat ttccgcgaac aaactgcccg gtatcaccat tttctgcatc
      361 cggcccccct tccgcgccgg tattcacaac cgtgtcggtt gccactgcgc cggatttgac
      421 ggcctgccag cccttctcat taatgacggt acccgttgca atcccgcctt catgtaccca
      481 ctgctcaccg ccgttcagag tggtattcac tgcctgcccc tgaaggctct gtccgcctcc
      541 ggcactgata accgtgtctg aaacgcttcc tccggcattc actctctgaa gaccaccacc
      601 ggtgacagta gtgttgttgg cgataccgcc attttgtatc cattgtccgc cggtattggc
      661 ctcgttatcc ggcccatact ccagaccggt actgatggtc attccgttgg ccgtaccgaa
      721 gacaatctgg ttgtcatgat tttccagtgt tccaccgttc acggtttctc ccgcctggac
      781 aaccgtgtca gcagccagtg ccgggagtga cgtgacagca gcaagagaca gagcaactgc
      841 cacaccggtg cgttttcccc gtgagcgcgc cagttcggaa gccaccacca gggtgcccgt
      901 aatgtgattc cataccagcc tgtagctggt gttcagatgt cgtttcatca gcttttcctt
      961 acagagggtg aataaaaaag ccggtaccca caaatgtgga taccggcagg tacaaaacag
     1021 catggtcaga taaagtggtt tttcccggac ataaatgctt cccctctctc atccgccaga
     1081 gaagtatcag tccgttgtgt gcaattccgt tctgcatgat gaagagattc agctgcattt
     1141 tcgcagacca ttaaccacta taaacgatcg atagtaacga tcgatacctg ttttatcgat
     1201 cgttttattc tattaacagc aaggcagtca cgcaagagaa agaggtgggt ctgaggtgtt
     1261 tgtgtcatgt tttcagttgg ttaacgcaat gtaatcaggc atttcccctg gcagagtgtt
     1321 cagaagaaaa tccagttcca tacagcgcgg gcaacagaga ccaccgagtc acggacggca
     1381 cgcagaaccg cgcgtgcaac agaggcaata cagacgctct ccgcagtgtc aaatatccgg
     1441 tccaccgcac cggtaaactg ttcacgggcc tgagaccgga cagattccgt gcgcagctcg
     1501 tcctgcaggc gggtcatcag gggactggcg gcatggtcgg gaagcgctgt catgagcgca
     1561 ctgaccagcg tatccagttc ccagccggtg caggccgata cggccacaac cggatgtacg
     1621 ggccggaaca gccggaatac cgcatctgtt ttctc
//
LOCUS       DAHHTG010000284         1642 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.292, whole genome shotgun sequence.
ACCESSION   DAHHTG010000284 DAHHTG010000000
VERSION     DAHHTG010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1642)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1642)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1642
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.292"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            70..342
                     /locus_tag="NBI74_004645"
     CDS             70..342
                     /locus_tag="NBI74_004645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001322642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin biosynthesis transcription regulatory
                     family protein"
                     /protein_id="HCD1934846.1"
                     /translation="MTPSPYPRNNFNTELIQSCMNGEHFSLLIEISPIRSKKNIMALK
                     EYLVDGYSKQESCERNNVSISYFCLCLKKLNYTHSIVSALKYFYRE"
     gene            788..963
                     /locus_tag="NBI74_004646"
                     /pseudo
     CDS             788..963
                     /locus_tag="NBI74_004646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612254.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(1189..>1642)
                     /locus_tag="NBI74_004647"
     CDS             complement(1189..>1642)
                     /locus_tag="NBI74_004647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001072358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1934847.1"
                     /translation="SKRISSAFVASLMILLVISTQITVLPVIFTHSVIKLSGISDFKI
                     HSYIIKTSEYPEEFFSNAVWGKKNIKPGEYYSVQAVSMFTTNQFILLCPKDIIRFYRE
                     SWKFDLLNVDFDTNTRKKLQEEAAYCVPISAISVKRWDMPLQGSKPSN"
BASE COUNT          510 a          303 c          292 g          537 t
ORIGIN      
        1 acttactcca atagcatttc tgtctattct atttcaattc tttaatagaa aaataaggtt
       61 agataatata tgactccctc cccctatcca agaaataact ttaatacaga gctaattcaa
      121 tcatgtatga atggagaaca cttttcactt ttaattgaaa tatcaccaat cagaagtaaa
      181 aaaaacatca tggctcttaa ggagtatttg gttgatggtt attcaaagca agaaagttgt
      241 gaaaggaaca atgtttccat tagttatttt tgcctatgcc taaaaaaact aaactataca
      301 cacagtattg ttagtgctct taaatatttc taccgtgagt aaactgtaac tgaagtcttg
      361 attcttacgc ttagacactg ctaagccgtt atggcatagc tgataattta tattgcacta
      421 tgtcagctac tgtaagataa tataagctat tttaaagaaa actctgtcat tcatcatttt
      481 gtttaatgat aggttttgtt attaatataa aagaacatgc tcattcatta aaataaaaac
      541 aagatgttct tttattattt tcattcccta cggggttagt ccttgattca ttttcagtat
      601 cttctaactc attcatttgc cgattacttt aattcacagt cttgtttttt atcctgccta
      661 ttggagataa aagtggtatc gatggatgcc gagtgtgttg cacatgcagt attctcagga
      721 ataagaactg tcacttccaa aacacgcgat atatgattga atctctgtgg ggggataaaa
      781 acttgccttt ttccgcagtc tgaaaacagg atgggggcga cggatggtta cgcaggcaag
      841 ggcgaggttc ggaaataaat cagagattat attctgaatt attacaatag caccagacct
      901 caccattaca acggtagttt gacgctggag ggatcagaaa acagataccg tttttactgt
      961 aaacggtggt catatatgga ctctcctggt gatataagta ctggttcgaa acggtttacg
     1021 actatatatc cggcgtcatc agtaggttca atgaaccgcg caataatgtt atccgagccc
     1081 tgtttcctaa tcactacgcc ggtatttgat tagccaagtc aagttcatct tgccatcact
     1141 atttttccat accgcattac tatctaatac agtcgcacga gcggccttct aatttgaggg
     1201 tttcgagccc tgaagaggca tatcccatcg ttttacagat attgcagaaa ttggtacgca
     1261 atatgctgct tcttcctgga gtttcttcct ggtatttgta tcgaagtcta cattaagcag
     1321 gtcgaacttc cagctttccc gataaaacct tataatatcc ttgggacaaa ggagaataaa
     1381 ctggtttgtc gtaaacatag aaacagcctg cacagagtaa tattcccccg gttttatatt
     1441 ttttttaccc catactgcat tggaaaaaaa ctcctccgga tattcacttg ttttaataat
     1501 gtagctatgt attttgaagt cacttatacc tgagagctta ataactgaat gggtaaaaat
     1561 tactggtaat acggttattt gagtcgatat aactaataat atcatcaagg atgcgacaaa
     1621 tgcagaacta attcttttag aa
//
LOCUS       DAHHTG010000285         1642 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.293, whole genome shotgun sequence.
ACCESSION   DAHHTG010000285 DAHHTG010000000
VERSION     DAHHTG010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1642)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1642)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1642
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.293"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            346..960
                     /gene="evgA"
                     /locus_tag="NBI74_004648"
     CDS             346..960
                     /gene="evgA"
                     /locus_tag="NBI74_004648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system DNA-binding response
                     regulator EvgA"
                     /protein_id="HCD1934848.1"
                     /translation="MNAIIIDDHPLAIAAIRNLLIKNDIEILAELTEGGSAVQRVETL
                     KPDIVIIDVDIPGVNGIQVLETLRKRQYSGIIIIVSAKNDHFYGKHCADAGANGFVSK
                     KEGMNNIIAAIEAAKNGYCYFPFSLNRFVGSLTSDQQKLDSLSKQEISVMRYILDGKD
                     NNDIAEKMFISNKTVSTYKSRLMEKLECKSLMDLYTFAQRNKIG"
     gene            965..>1642
                     /locus_tag="NBI74_004649"
     CDS             965..>1642
                     /locus_tag="NBI74_004649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HCD1934849.1"
                     /translation="MKFLPYIFLLCCGLWSTISFADGDYIEYRGISSNNRVTLDPLRL
                     SNKELRWLASKKNLVIAVHKSQTATLLHTDSQQRIRGINADYLNLLKRALNIKLTLRE
                     YADHQKAMDALEDGEVDIVLSHLVASPPLNDDIAATNPLIITFPALVTTLHDSMRPLT
                     SSKPVNIARVANYPPDEVIHQSFPKATIISFTNLYQALASVSAGQNDYFIGSNIITSS
                     MISRYFTH"
BASE COUNT          519 a          326 c          312 g          485 t
ORIGIN      
        1 cacagtgcat gttttgcaca ttatcccagg aacgttggag ggctaattta aatgaggtgc
       61 aaaaaaaaca acagaggact aaaccgcggc ttttgcaata caattcttac gcctgtagga
      121 ttagtgagaa aacttatagt gctcatttga aactataaat catcggtatc atccctgatt
      181 ttattgttga cattttattt atgccgacta tttatatggt atacttgtcg aattatctta
      241 aaggaagctc aaattttctt atttttattg agaaaatgag atgatacctt atgtctgtat
      301 tactacaggg agaagggaaa tgcttcattg caaagggaat aatctatgaa cgcaataatt
      361 attgatgacc atcctcttgc tatcgcagca attcgtaatt tattgatcaa aaacgatatt
      421 gaaatcttag cagagttgac tgaaggcgga agcgccgttc agcgggtgga aacacttaag
      481 cctgatatcg tcatcattga tgtcgatatc cccggagtta acggtatcca ggtgttagaa
      541 acgctgagaa agcgccaata tagcggaatt attattattg tctccgctaa aaatgaccat
      601 ttttacggga aacattgtgc tgatgctggc gctaatggtt tcgtgagtaa aaaagaaggc
      661 atgaacaata tcattgcagc tattgaagct gcaaaaaatg gctactgcta tttccccttc
      721 tctctcaacc ggtttgttgg aagtttaacg tccgaccagc aaaaactcga ctccttatcg
      781 aaacaagaaa ttagtgtcat gcggtatatt cttgatggca aggataataa tgacattgct
      841 gaaaaaatgt tcatcagcaa caaaactgtc agcacttata aaagtcgctt gatggaaaaa
      901 ttagaatgca aatcactgat ggatctttac acattcgcac aacgtaacaa aatcggctaa
      961 ccacatgaag tttttaccct atatttttct tctctgttgt ggtctttggt cgaccataag
     1021 tttcgcagac ggagattaca tcgaatatcg tggcatcagt agtaacaacc gtgtcacact
     1081 tgatccacta cgtctgagca acaaggaatt acgttggcta gcgagcaaaa aaaatcttgt
     1141 gattgcagta cacaagtccc aaacagctac gttgttgcat accgattcgc agcaacggat
     1201 tcgtggtatt aatgctgatt atttaaatct tttaaaaaga gcgttaaata tcaaattaac
     1261 actccgggaa tacgcagatc atcaaaaagc aatggacgcg ctggaagacg gtgaagttga
     1321 tatagtgtta tcacatttag ttgcttcgcc gcctcttaat gatgacattg ctgcaactaa
     1381 tcctctgata attacctttc cggcgctggt cactaccctt cacgattcaa tgcgaccgct
     1441 tacctcatca aaaccagtaa atattgctcg agtagcaaat tatcccccag acgaggtaat
     1501 tcatcaatca tttccaaaag caacaattat ctcttttaca aatttatatc aggcattagc
     1561 atccgtctca gccggacaga atgattactt tattggtagt aacatcatta ccagcagtat
     1621 gatttcccgt tattttactc ac
//
LOCUS       DAHHTG010000286         1633 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.294, whole genome shotgun sequence.
ACCESSION   DAHHTG010000286 DAHHTG010000000
VERSION     DAHHTG010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1633)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1633)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1633
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.294"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            complement(48..1034)
                     /locus_tag="NBI74_004650"
     CDS             complement(48..1034)
                     /locus_tag="NBI74_004650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000274668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1934850.1"
                     /translation="MYHHDFNEKIGFWYVIALAGQSNGMAYGEGIPLPDTLDKPESRV
                     KQLARRKTITPGGKECKFNEIIPADHCLHDVQDMSGYHHPAADLHKGEYGCVGQGLHI
                     AKKLLPYIPEQAGILLVPCCRGGAAFTVGAEGMYVPDTGATADAMRWGTGTALYEDLV
                     ARVKVALEYNRKNKLLSVCWMQGEFDLMSPDYEKHPDLFYQMVTSFRSELSEYSSQCV
                     GNSSERVPWLCGDTTWYWKESYQKEYDFIYGHYRQRTDDEIHFLSFQDSNRHELTNEP
                     EEDADDLSVGYLGSSWRTELSWTTSQRSTHFNSMARRGVIAECYAQKIRNYL"
     gene            1134..>1633
                     /locus_tag="NBI74_004651"
     CDS             1134..>1633
                     /locus_tag="NBI74_004651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001282578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCD1934851.1"
                     /translation="MSDNKKTVLPGLTTTRKPIVTKKLSEIVAEELERMIRQEEIREG
                     EYLPSERELMAFFGVGRPSVREALSSLRHKGLIRYNKGEKASVCRPSPETIINGLSGI
                     VKDFLSTADGIKDFERLRIFFETNLVRYAAKNATPEDIHRLEKVLSIHHNSLRTPEVY
                     AQYDIE"
BASE COUNT          489 a          374 c          294 g          476 t
ORIGIN      
        1 gtctctcaga gaatcatgtc agagctgtag atatatcctg aacttgtcta caaataattt
       61 cttatttttt gcgcataaca ttcagcaata actccccttc tggccattga attaaaatgt
      121 gtacttctct gtgacgttgt ccagcttaat tctgtcctcc atgatgaccc cagatacccg
      181 acagataaat catcggcatc ttcttctggc tcattagtta attcatgtcg gttactatcc
      241 tgaaagctga gaaaatgtat ttcatcatca gtccgttgtc tgtaatgtcc atatataaaa
      301 tcatattctt tttgataaga ttccttccag taccaggttg tatcaccaca caaccatgga
      361 actctttctg acgaattacc aacacactga gatgagtatt cagataactc agacctgaaa
      421 gaagttacca tctgataaaa caagtcagga tgcttttcat aatctggtga cataagatca
      481 aactccccct gcatccagca gacagatagc agtttattct ttctgttata ttccagagct
      541 accttaaccc gggctactaa gtcctcatag agtgctgttc cggtgcccca ccgcattgca
      601 tcagctgttg cccctgtatc aggtacatac ataccttcag ctccaacagt aaaggcggca
      661 cctcctctgc aacaaggaac cagtaatatc cctgcttgct caggtatgta tggcagtaac
      721 tttttagcta tatgcaatcc ctgtccgaca caaccatatt cccctttatg caaatcagca
      781 gccggatggt gatatccact catatcctga acatcatgca ggcagtgatc tgccggaata
      841 atctcattga atttacactc ttttcctcct ggtgtgattg tttttcgacg agccagttgt
      901 tttacacggg actcgggttt gtcgagtgta tctggcaggg gaataccttc accgtaggcc
      961 ataccattag actgtcctgc gagggctatc acataccaga aacctatttt ttcattaaaa
     1021 tcatgatgat acatcattcc ccctttacat aatagattta attaaatatt ttcattatta
     1081 aaagaaagtg ttaaattaaa aactcatttg ctctaatcca tataaatcaa tatatgtccg
     1141 acaacaaaaa aaccgtgtta cccgggctaa caacgaccag aaagcccatt gttacaaaaa
     1201 aactttcaga aatcgttgcc gaagagcttg aaagaatgat ccgccaggag gagataaggg
     1261 aaggtgaata cctgccttct gaacgtgaac ttatggcatt ctttggcgtt ggaagaccat
     1321 ctgtcagaga ggctttatca tctctcagac ataaagggct aatccggtat aacaagggag
     1381 aaaaagccag tgtctgccgt ccatcaccag aaaccatcat taatgggtta tcaggcattg
     1441 ttaaagattt tttatctaca gctgatggca tcaaggattt tgaaagacta cgcatctttt
     1501 ttgaaaccaa ccttgtaaga tatgcagcaa aaaatgcaac gcctgaggat attcacaggc
     1561 ttgagaaggt gctcagtatc catcataact cactgagaac ccccgaagtg tatgctcaat
     1621 atgatattga att
//
LOCUS       DAHHTG010000287         1626 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.295, whole genome shotgun sequence.
ACCESSION   DAHHTG010000287 DAHHTG010000000
VERSION     DAHHTG010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1626)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1626)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1626
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.295"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            <1..1596
                     /locus_tag="NBI74_004652"
     rRNA            <1..1596
                     /locus_tag="NBI74_004652"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          404 a          360 c          514 g          348 t
ORIGIN      
        1 gttcctgtcc aacgttaatc ggggcagggt gagtcgaccc ctaaggcgag gccgaaaggc
       61 gtagtcgatg ggaaacaggt taatattcct gtacttggtg ttactgcgaa ggggggacgg
      121 agaaggctat gttggccggg cgacggttgt cccggtttaa gcgtgtaggc tggttttcca
      181 ggcaaatccg gaaaatcaag gctgaggcgt gatgacgagg cactacggtg ctgaagcgac
      241 aaatgccctg cttccaggaa aagcctctaa gcatcaggta acatcaaatc gtaccccaaa
      301 ccgacacagg tggtcaggta gagaatacca aggcgcttga gagaactcgg gtgaaggaac
      361 taggcaaaat ggtgccgtaa cttcgggaga aggcacgctg atatgtaggt gaagcgactt
      421 gctcgtggag ctgaaatcag tcgaagatac cagctggctg caactgttta ttaaaaacac
      481 agcactgtgc aaacacgaaa gtggacgtat acggtgtgac gcctgcccgg tgccggaagg
      541 ttaattgatg gggttagcgg taacgcgaag ctcttgatcg aagccccggt aaacggcggc
      601 cgtaactata acggtcctaa ggtagcgaaa ttccttgtcg ggtaagttcc gacctgcacg
      661 aatggcgtaa tgatggccag gctgtctcca cccgagactc agtgaaattg aactcgctgt
      721 gaagatgcag tgtacccgcg gcaagacgga aagaccccgt gaacctttac tatagcttga
      781 cactgaacat tgagccttga tgtgtaggat aggtgggagg ctttgaagtg tggacgccag
      841 tctgcatgga gccgaccttg aaataccacc ctttaatgtt tgatgttcta acgtgggccc
      901 cttaccgggg ttgcggacag tgtctggtgg gtagtttgac tggggcggtc tcctcctaaa
      961 gagtaacgga ggagcacgaa ggttggctaa tcctggtcgg acatcaggag gttagtgcaa
     1021 tggcataagc cagcttgact gcgagcgtga cggcgcgagc aggtgcgaaa gcaggtcata
     1081 gtgatccggt ggttctgaat ggaagggcca tcgctcaacg gataaaaggt actccgggga
     1141 taacaggctg ataccgccca agagttcata tcgacggcgg tgtttggcac ctcgatgtcg
     1201 gctcatcaca tcctggggct gaagtaggtc ccaagggtat ggctgttcgc catttaaagt
     1261 ggtacgcgag ctgggtttag aacgtcgtga gacagttcgg tccctatctg ccgtgggcgc
     1321 tggagaactg aggggggctg ctcctagtac gagaggaccg gagtggacgc atcactggtg
     1381 ttcgggttgt catgccaatg gcactgcccg gtagctaaat gcggaagaga taagtgctga
     1441 aagcatctaa gcacgaaact tgccccgaga tgagttctcc ctgactcctt gagagtcctg
     1501 aaggaacgtt gaagacgacg acgttgatag gccgggtgtg taagcgcagc gatgcgttga
     1561 gctaaccggt actaatgaac cgtgaggctt aaccttacaa cgccgaagat gttttggcgg
     1621 aagaga
//
LOCUS       DAHHTG010000288         1622 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.296, whole genome shotgun sequence.
ACCESSION   DAHHTG010000288 DAHHTG010000000
VERSION     DAHHTG010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1622)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1622)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1622
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.296"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
BASE COUNT          449 a          306 c          375 g          492 t
ORIGIN      
        1 aagttaatct actggagatg aagtaacttc attaatgcgt cataaaaata aaaattcctg
       61 ttttgggaaa tatgaatgtg tctgtcccct ccttcgggag ggtgtttttt atctggaaac
      121 tggtttctgg ttctgatttt tctttttgta ttatagcgag ttacgcatat ttaccaatcg
      181 atgtctggca aacagagtat ggcgatggtg tgatttgtgt ttattggtaa aaaaaacacc
      241 cccgtagtgg gggtgttgag acttcaacct atgaactcat tatcctggga tggatgagta
      301 gcgtgcgata ttgaatcata ttcagcagga ataataatat tcgcaaaaaa atggatgtgc
      361 aaggaaattt tttatatgct tttgtgtgtg gtggttaggt aatcaagaag ataaatgatg
      421 aagtgtgcca gttcaggatc aatgtttcca tgggaaagcc atttattaat aatatcaatt
      481 aagttcgaaa gctcagggaa gatttgttca atgttttcct ctgtctcccg cacagtcata
      541 tagcgacgtc tccttgggga ttgaaaacaa aaacaataat acaacaagta tgtaacagaa
      601 ggtaggtggc aggggcggaa caggaaaacg catgagggcg cttcctgggg gagatgcctg
      661 gtatctttag ggttcagttt tgagatcggg aatttttgta cattaagtgc attatttaat
      721 caattgaaac gcgtaatgga ttgattttat cagcaactat cgcgtttaag ttgtcgatca
      781 tatttgagtt gtctcattaa ggtcgaatcc cctgaaagcg gcgtataacg taggagttcc
      841 cctctatagc aataggtaag gctgttcaaa aaaggcttac cgtacaataa ttcgctaggc
      901 cccccttgtc atacaagctc gtaagcggcc ctgacggcct gtcaatgcgg tactgccctg
      961 tcgggactgc tctacccaca taagtttcta catagcttga agctggatgg ggaggtatta
     1021 cggctttagc cgtaatactt tcttcgtcct cgcagcgctg acagttttca gggatcaaat
     1081 ttaggtccgc catacactcc gtaaagactc tgtcagttca caggtattac ctagtgaaga
     1141 cgtatcataa aatgaaaact gtagttgggt agttgctccc cctcacggca tgcaggggct
     1201 gagggggaaa atttccttat cttgttgttg ctatttctgg cttagtcagt tttgaatttt
     1261 gcttcctaag caataatccc ctcaccgcaa caatcgttct tctacactaa taagatccga
     1321 aaaaaggtag tcacggatct tcctctcccg ccgcacagcg ccatctcccg tcagcacaac
     1381 atgtagtgct gaatttagct gttaatgaca ctatatgttg tgccatctcc ctgacctgtg
     1441 atgcgccgcg caggggcgga aaacagtgat atgatgattt tctcagcgtg gtacacttcc
     1501 ggaaagttgt gatattaatg aaagtcggat ctgacggaaa cgggaatgcg ataattacgg
     1561 tttgttttta taagccagat tttgattttt attggttaac ataacgcaag gtaaaataaa
     1621 ta
//
LOCUS       DAHHTG010000289         1579 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.297, whole genome shotgun sequence.
ACCESSION   DAHHTG010000289 DAHHTG010000000
VERSION     DAHHTG010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1579)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1579)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1579
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.297"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            35..496
                     /locus_tag="NBI74_004653"
     CDS             35..496
                     /locus_tag="NBI74_004653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HCD1934852.1"
                     /translation="MRKYIPLVLFIFSWPVLCADIHGRVVRVLDGDTIEVMDSRKAVR
                     IRLINIDAPEKKQDYGRWSTDMMKSLVAGKTVTVTYFQRDRYGRILGQVYAPDGMNVN
                     QFMVRAGAAWVYEQYNTDPVLPVLQNEARQQKRGLWSDADPVPPWIWRHRK"
     gene            542..751
                     /gene="hha"
                     /locus_tag="NBI74_004654"
     CDS             542..751
                     /gene="hha"
                     /locus_tag="NBI74_004654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin expression modulator Hha"
                     /protein_id="HCD1934853.1"
                     /translation="MAKTKQEWLYQLRRCSSVNTLEKIIHKNRDSLLNSERESFNSAA
                     DHRLAELITGKLYDRIPKEIWKYVR"
     gene            789..1379
                     /locus_tag="NBI74_004655"
     CDS             789..1379
                     /locus_tag="NBI74_004655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2726 domain-containing protein"
                     /protein_id="HCD1934854.1"
                     /translation="MKLIIFILIVLIIAALLIRIILKSVNQHSPLLMQLHAAGIRTGD
                     AERILSGGEYWQRQKTLLTEREVSFMKGLFRIVDMKRWYLCPQVRVADIVQLNGNIRP
                     RSRQWWQLFRMVSQWHVDVVIVERRSFSIVAAVELDDASHLRPERRRRDILLEEVLRQ
                     AGIPLLRSHDARKLLQMTGEWLNTTGADQQSPEHRS"
BASE COUNT          458 a          309 c          413 g          399 t
ORIGIN      
        1 atacaggtgc catttatctg atatggagaa taacatgagg aaatatattc cactggtatt
       61 atttatcttt tcatggccgg tattgtgtgc agatattcat ggacgggttg ttcgtgtact
      121 ggacggtgat accatcgagg tcatggactc acggaaggca gtgcggatca ggctgataaa
      181 tattgatgca ccggaaaaga aacaggatta tggccggtgg tcaacggaca tgatgaaatc
      241 actggttgca ggaaaaacgg ttaccgtcac atattttcag cgagatcgtt atggtcgtat
      301 tctcgggcag gtttatgcac cagacggaat gaatgttaat cagtttatgg tccgggccgg
      361 ggctgcctgg gtatatgagc agtacaacac agatcccgtg ctgcctgtcc ttcagaacga
      421 agccagacaa caaaaacgtg ggctctggtc agatgctgat ccggttcccc cctggatatg
      481 gagacaccgt aaataaagcg atggcaatct tatcattcat ctgttctcag cgggaataaa
      541 aatggcgaaa acaaaacagg aatggctgta tcagttacgg cgatgttcat cagtcaatac
      601 gctggaaaaa attattcata aaaacagaga cagtttatta aacagtgaac gtgaatcatt
      661 taattcagca gcagaccacc ggttagcgga attaatcacc ggaaagttat atgacaggat
      721 accaaaagag atatggaaat atgtaaggta ataattcaga ataattaatt accaggagca
      781 gataaacaat gaagctgatt atttttattc tcattgttct gattattgca gcgttactta
      841 tccggattat cctcaaaagt gtaaatcagc attctccttt gctgatgcag ttgcatgcgg
      901 caggtatacg gaccggtgat gcagagcgaa tactgtccgg cggtgaatac tggcaacgtc
      961 agaagacgct gctgacagaa agggaagtca gttttatgaa aggactgttc agaattgtgg
     1021 atatgaagcg gtggtatctg tgtccgcagg tacgggtcgc ggatatcgtc cagctgaacg
     1081 ggaatatccg gccacgatca cgccagtggt ggcagttatt caggatggtg tctcagtggc
     1141 atgttgatgt ggttatcgtt gaacggcgtt cgttcagtat tgttgcagca gtagagctgg
     1201 atgatgccag ccatttacga ccggaacgcc ggcgccggga tattcttctg gaagaggttc
     1261 tgaggcaggc tggtattccg ttgctcagaa gccacgatgc cagaaaactg ctgcagatga
     1321 ccggagaatg gctgaataca acaggggctg atcagcagtc cccggaacat cgtagctgac
     1381 gccttcgcgt tgctcagttg tccaaccccg gaaacgggaa aaagcaagtt ttccccgctc
     1441 ccggcgtttc tgtaataaaa agcgggaaat ggtggtcaca gagtaaattc cggaaccacc
     1501 tgtacccaaa ctttatataa taatggtgaa tatatgaaat atgatgatgt atcagaattg
     1561 atgattaagc gtgaacaca
//
LOCUS       DAHHTG010000290         1566 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.298, whole genome shotgun sequence.
ACCESSION   DAHHTG010000290 DAHHTG010000000
VERSION     DAHHTG010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1566)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1566)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1566
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.298"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            complement(<1..391)
                     /locus_tag="NBI74_004656"
     CDS             complement(<1..391)
                     /locus_tag="NBI74_004656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001606949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2931 family protein"
                     /protein_id="HCD1934855.1"
                     /translation="MEFNIKLLFVITSMVLNFNAAAKEKISLLAPYDEWYFNFFYPNS
                     LPAEVTYVELLDTDGIYYQFRTLASTNASPGSVGQWSEKVSGMSSVFNKAKNPPMSIH
                     FCWDSVIDKKVYETWITLAGDVWKLMLT"
     gene            complement(381..>1566)
                     /locus_tag="NBI74_004657"
     CDS             complement(381..>1566)
                     /locus_tag="NBI74_004657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001524195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2235 domain-containing protein"
                     /protein_id="HCD1934856.1"
                     /translation="TENNKADSLVGMGLGNNDTGVIAKTDSAISLLRETLAKIIVDLK
                     DAKLVIKRLQFDVFGFSRGAAAARHFTNRVFERDPVLIKTIATAFQSVEYLGKPSGEV
                     QFLGIFDTVAAIGGLMDGFDPHDGNNFPVKVKLPPGVAKHVFQLTAMNECRYNFCLNS
                     IKESWPELSFPGSHADIGGGYDPLEDEYLFLSRPAIETVLGNVPVEATSAYQKAAQQA
                     ELLWRHSTLAPILPSGVLKIESNIDEIASPDHLGNVRKRVAAAVTFRRTITNDWSKIA
                     LRVMYEVAKEAGVVFDAISVADKELKVPSHLKVICEKAISQGKAVLLCTAPMPFSDDE
                     LKTIGKYLHCSSNWNTVDYRLKNKVSAEVSAFKTFSFVNRPDENWTRTIYDMQGNKQN
                     GI"
BASE COUNT          438 a          368 c          291 g          469 t
ORIGIN      
        1 gtgtgagcat caatttccag acgtctcccg ccagtgttat ccaggtttca tacacttttt
       61 tatcaatcac tgaatcccag caaaaatgga tagacattgg tgggtttttg gctttattaa
      121 aaactgacga catccctgat actttctcgc tccattgtcc aacacttcct ggactggcat
      181 tagtactcgc cagagttctg aactgataat agatgccatc ggtatctaat aactccacat
      241 aggtaacttc tgctggtagg gaattagggt aaaaaaaatt gaaataccat tcatcatagg
      301 gtgcaagcag tgatattttt tctttcgctg ctgcgttaaa gttaagaacc atagacgtaa
      361 taacaaacag taattttata ttaaattcca ttttgtttat tcccttgcat atcgtatata
      421 gttctggtcc aattttcatc cggccgatta acaaaactaa acgtcttaaa cgctgatact
      481 tcagcagaca ccttattttt taatcgataa tcgacggtat tccagttgga agagcagtgt
      541 aaatatttcc caatggtttt aagctcatca tcagaaaaag gcatcggcgc tgtacacaag
      601 agaacggctt taccctgaga aattgctttt tcacatatta cctttaaatg actgggaact
      661 tttaattcct tatcagcaac agaaatggca tcaaatacta cccctgcctc tttagcgact
      721 tcatacatta ctcttaacgc aatctttgac cagtcattgg tgatcgttct gcgaaatgtc
      781 accgctgcag cgacccgttt tctcacatta cctaagtggt caggcgacgc tatttcatca
      841 atattggatt caatttttag gacaccagaa ggcagaatcg gagccagcgt tgaatggcgc
      901 cacaataact ctgcctgctg tgctgctttt tggtaagcgc ttgtcgcttc caccggcaca
      961 tttcccagta ccgtttcaat cgccgggcga cttaaaaaca ggtactcatc ttccagtggg
     1021 tcatagccgc caccaatatc agcatgtgat ccagggaaac ttaattctgg ccagctttcc
     1081 tttatgctgt tgaggcaaaa gttatagcga cattcgttca ttgctgtgag ctggaataca
     1141 tgtttggcca cacctggcgg taacttcact tttaccggaa agttattacc atcatgaggg
     1201 tcaaaaccat ccatcagccc tccgatggct gcaactgtgt caaaaatacc taaaaattgc
     1261 acttccccgg aaggtttacc taaatattca actgactgaa atgccgtcgc aatggtttta
     1321 atcagtacgg gatcacgttc aaatacgcga ttagtaaagt gccgtgcggc tgccgcccca
     1381 cgactaaagc caaatacgtc aaactgtaga cgcttaatta ctagtttagc atcctttaaa
     1441 tcaacaataa tctttgccag tgtctcacga agcagtgata tcgcactgtc agttttggca
     1501 attaccccag tatcattatt tcctagcccc attcctacca gagagtctgc tttattattt
     1561 tcagtg
//
LOCUS       DAHHTG010000291         1558 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.299, whole genome shotgun sequence.
ACCESSION   DAHHTG010000291 DAHHTG010000000
VERSION     DAHHTG010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1558)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1558)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1558
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.299"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            119..463
                     /locus_tag="NBI74_004658"
     CDS             119..463
                     /locus_tag="NBI74_004658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415690.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper family protein"
                     /protein_id="HCD1934857.1"
                     /translation="MKKKILAFGLISALFCSTPAMADMNRTTKGALLGAGVGLLTGNG
                     VNGVLKGAAVGAGVGAVTEKGRDGKNARKGAKVGAAVGAVTGVLTGNGLEGAIKGAVI
                     GGTGGAILGKMK"
     gene            467..802
                     /locus_tag="NBI74_004659"
     CDS             467..802
                     /locus_tag="NBI74_004659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmgD family protein"
                     /protein_id="HCD1934858.1"
                     /translation="MMMKKFALLAGLFVFAPMTWAQDYNIKNGLPSETYITCAEANEM
                     AKTDSAQVAEIVAVMGNASVASRDLKIEQSPELSAKVVEKLNQVCAKDPQMLLITAID
                     DTMRAIGKK"
     gene            complement(861..1460)
                     /locus_tag="NBI74_004660"
                     /pseudo
     CDS             complement(861..1460)
                     /locus_tag="NBI74_004660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707067.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
BASE COUNT          402 a          345 c          396 g          415 t
ORIGIN      
        1 atcacagtca cgctcatttt gaattatctg aataatagcg agcgttcttt agttaagcag
       61 atcttaattg aagacgaaat tattgtgtgt gcaacttact taataccgga caactaagtt
      121 gaagaaaaaa atactcgcct tcgggcttat ctcagcattg ttctgctcca cgccagcaat
      181 ggcggatatg aatcgcacca cgaaaggtgc attgctaggt gcaggcgttg gtttacttac
      241 tggcaatggt gttaatggcg tactgaaagg tgcagctgtt ggcgctggtg ttggtgcagt
      301 aacagaaaaa ggccgtgacg gtaaaaatgc gcgtaaaggt gcaaaagtgg gtgccgccgt
      361 tggtgctgtc actggcgttc tgaccggcaa tggcctcgaa ggggcgatta aaggtgccgt
      421 gattggcggt acgggtggtg caattctggg taaaatgaaa taaggaatga tgatgaaaaa
      481 atttgcttta ctggcgggtc tgtttgtttt cgcaccaatg acctgggcgc aggactacaa
      541 tattaagaac ggtttgccat cagaaaccta tattacttgc gcagaagcca acgaaatggc
      601 aaaaactgac agcgcacagg ttgcagaaat tgttgcggta atgggtaatg ccagcgttgc
      661 cagccgtgat ttaaaaattg agcaatctcc ggaactgagc gccaaagtgg tagaaaaact
      721 gaatcaggtt tgcgctaaag atccgcaaat gctgttgatc acggcaattg atgacaccat
      781 gcgtgctatc ggcaaaaaat aatatttcat atgttaccgc gtcgatgaat tcgacgcggt
      841 tttttagcta acgggcggga tcagaactta tatttcaggc ccaccatcat tgcggtgtca
      901 ttgtagccat tatcacccag ctgcacgccc acattacccc agacgctggc cgccggattc
      961 agattgccat ttacgccatg tttcatttcc cccagattac gagccccgtc ttgttcgact
     1021 ttcacaccgt tcattgagac agcaaaatct ttactgttat ggatcccgtt catttcaaca
     1081 aaaggttcaa agtcgtcacc gtgcggaccg ctgtttttct cctgagtacg aataaaggtg
     1141 cggaaaccaa gtcggctctg gatgttgtaa tttgcgtcat tctcaatacg tgagccattg
     1201 gcttccgtgt gcgcattatg atcgacgccc atccagatgg cctgtgccga ggtcgttctc
     1261 aactgtccgt tgctgtcacg ccaggcatta tgcccgccaa tttgacatag ccaaagttgg
     1321 cttgaacgct cctgttcagt tacaggatcg atatagcgcg tttcacccgc acgatcgttc
     1381 agacgcgtaa caaaaagagt gttagcggct gccaggttgg cgacgtaact gccagcgtcg
     1441 ggtcgcagga cgggggtagg cgtttctgtt gattctgaag tgacattgga aaaaaatcac
     1501 tctctaccat tgtatatttt tgcaatgggg gctttagtgc catttgttgt aatagaac
//
LOCUS       DAHHTG010000292         1550 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.300, whole genome shotgun sequence.
ACCESSION   DAHHTG010000292 DAHHTG010000000
VERSION     DAHHTG010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1550)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1550)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1550
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.300"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            complement(<1..742)
                     /locus_tag="NBI74_004661"
     CDS             complement(<1..742)
                     /locus_tag="NBI74_004661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HCD1934859.1"
                     /translation="MTKSETFMIPNHKAAKLSELDMMIVNSVPPGGNWKNIPLDVPSK
                     RIEQIRDSYAQGKGSRSTYYGRLLPDMPAYTINTYFNRPGNGCHIHYEQDRVLSQREA
                     ARLQSFPDDFIFFGGQTAINTQIGNAVPPFLAFLIAKEIEKAIGNTGYYIDLFSGAGG
                     LGLGFKWAGWTPLLANDIEEKYLQTYSNNVHKEVLCGSISDNETFSKIADKISGFKKL
                     YFDKQLWILGGPPCQGFSTAGNARTMDDP"
     gene            complement(735..>1550)
                     /locus_tag="NBI74_004662"
     CDS             complement(735..>1550)
                     /locus_tag="NBI74_004662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Alw26I/Eco31I/Esp3I family type II restriction
                     adenine-specific DNA-methyltransferase"
                     /protein_id="HCD1934860.1"
                     /translation="DNIQLSVYDKNLNIKSLGFFDLSSIDSQYLSIPVSQGVHAVHLL
                     RKLQEGYPTWGSLEKNGEIWAGREIDETGSSDWTQKSGGGLLFIKGKMIGRYNFHNEK
                     SLRITKKIDKVLSNSNFVRIAWRDISRPSQKRRMIATIIPPNSLAGNSLGVVYYKSGS
                     QDSLFSLLGIINSLCFEFQLRSFLATGHVSLSALRKTAIPSEKILLQHSELKQLVISC
                     IEGCCDAELKIEAYVAKNIYKLDLNEFNKLLSSFDKIELAEKESLLRIFQHYD"
BASE COUNT          471 a          320 c          232 g          527 t
ORIGIN      
        1 ttggatcatc cattgttctg gcatttcctg ccgtagaaaa tccctggcat gggggtccac
       61 caagtatcca taattgttta tcaaaatata atttcttaaa tcctgatatt ttatctgcta
      121 tttttgaaaa agtttcatta tcagaaatgc tgccacataa aacttctttg tgaacattgt
      181 ttgaatatgt ttgtagatat ttttcttcaa tatcattcgc caataatgga gtccatccag
      241 cccatttgaa tcctagtccc agccctccag ctccggaaaa taaatcgata taataaccag
      301 tattacctat cgccttctct atttctttcg caatcagaaa agctaaaaat gggggcactg
      361 cattaccgat ttgggtatta attgctgttt gacctccgaa gaaaataaaa tcatccggga
      421 acgattgtag tcttgcagct tctctctggg atagaacacg atcttgctca tagtgaatat
      481 ggcaaccatt cccaggtcgg ttaaaatatg tattgatagt atatgcaggc atatctggaa
      541 gtaatctacc gtagtatgtt gaacggcttc cttttccttg tgcataacta tccctaattt
      601 gctctattct tttagacggt acatctaaag gaatattttt ccagttgcca cctggcggta
      661 ctgagttaac aatcatcata tccaattcac ttagctttgc tgccttgtga ttgggaatca
      721 taaatgtttc acttttagtc ataatgttga aaaatcctca ataaactttc tttttcagct
      781 aactcaatct tatcaaatga agacagaagc ttattaaatt cattcaaatc caatttgtaa
      841 atatttttgg caacataagc ctcaattttc aactcagcat cacaacatcc ctctatgcaa
      901 gaaatcacaa gttgttttaa ttcagaatgt tgtaataata ttttttcaga cggtattgca
      961 gtttttcgta aggctgacaa tgatacatga ccagtagcta aaaagcttct taattgaaat
     1021 tcaaaacaaa gggagtttat aataccaagc aaactaaaga gcgaatcttg cgaaccactt
     1081 ttataataga ccacccctaa tgaattccct gccaaagagt taggaggtat tatcgttgca
     1141 atcattcgcc ttttttgact cgggcgtgaa atgtcacgcc atgcaatcct tacgaaatta
     1201 gaattggata gaaccttatc aatttttttc gttattctta agctcttctc attatgaaaa
     1261 ttatacctcc caatcatttt tcctttaatg aataataatc cgccgccact tttttgtgtc
     1321 cagtcactac taccagtttc atctatttct cttcctgccc atatctctcc atttttttcc
     1381 agactacccc atgttgggta tccctcttgc agctttctta acaaatgaac ggcatgaaca
     1441 ccttgagata ccggaatgct aagatattga gaatctatac tagacaaatc aaaaaaacct
     1501 agtgatttaa tatttaagtt tttatcatac acactaagtt gaatattatc
//
LOCUS       DAHHTG010000293         1542 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.301, whole genome shotgun sequence.
ACCESSION   DAHHTG010000293 DAHHTG010000000
VERSION     DAHHTG010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1542)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1542)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1542
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.301"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            <1..>1542
                     /gene="sat"
                     /locus_tag="NBI74_004663"
     CDS             <1..>1542
                     /gene="sat"
                     /locus_tag="NBI74_004663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001034089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease autotransporter toxin Sat"
                     /protein_id="HCD1934861.1"
                     /translation="GGLITVSELAKRVSGKTNRKLVATMLSLAVAGTVNAANIDISNV
                     WARDYLDLAQNKGIFQPGATDVTITLKNGDKFSFHNLSIPDFSGAAASGAATAIGGSY
                     SVTVAHNKKNPQAAETQVYAQSSYKVVDRRNSNDFEIQRLNKFVVETVGATPAETNPT
                     TYSDALERYGIVTSDGSKKIIGFRAGSGGTSFINGESKISTNSAYSHDLLSASLFEVT
                     QWDSYGMMIYKNDKTFRNLEIFGDSGSGAYLYDNKLEKWVLVGTTHGIASVNGDQLTW
                     ITKYNDKLVSELKDTYSHKINLNGNNVTIKNTDITLHQNNADTTGTQEKITKDKDIVF
                     TNGGNVLFKDNLDFGSGGIIFDEGHEYNINGQGFTFKGAGIDIGKESIVNWNALYSSD
                     DVLHKIGPGTLNVQKKQGANIKIGEGNVILNEEGTFNNIYLASGNGKVILNKDNSLGN
                     DQYAGIFFTKRGGTLDLNGHNQTFTRIAATDDGTTITNSDTTKEAVLAINNEDSYIYH
                     GNINGN"
BASE COUNT          557 a          265 c          312 g          408 t
ORIGIN      
        1 ggcggactca ttactgtttc tgaattagcg aaaagagttt ctggtaaaac aaaccgaaaa
       61 cttgtagcaa caatgttgtc tctggctgtt gccggtacag taaatgcagc aaatattgat
      121 atatcaaatg tatgggcgag agactatctt gatcttgcac aaaataaagg tattttccag
      181 cccggagcaa cagacgtaac aatcacttta aaaaacggag ataaattctc tttccataat
      241 ctctcaattc cggatttttc tggtgcagca gcgagtggcg cagctaccgc aataggaggt
      301 tcttatagtg ttactgttgc acataacaaa aagaaccctc aggccgcaga aactcaggtt
      361 tacgctcagt cttcttacaa ggttgttgac agaagaaatt ccaatgattt tgagattcag
      421 aggttaaata aatttgttgt ggaaacagta ggtgccaccc cggcagagac caaccctaca
      481 acatattctg atgcattaga acgctacggt atagtcactt ctgacggttc aaaaaaaatc
      541 ataggttttc gtgctggctc tggaggaaca tcatttatta atggtgaatc caaaatctca
      601 acaaattcag catatagcca tgatctgtta agtgctagtc tatttgaggt cacccaatgg
      661 gactcatacg gcatgatgat ttataaaaat gataaaacat ttcgtaatct tgaaatattc
      721 ggagacagcg gctctggagc atacttatat gataacaaac tagaaaaatg ggtattagtc
      781 ggaacaaccc atggtattgc cagcgttaat ggtgaccaac tgacatggat aacaaaatac
      841 aatgataaac tggttagtga gttaaaagat acctatagtc ataaaataaa tctgaatggc
      901 aataatgtaa ccataaaaaa cacagatata acattacacc aaaacaatgc agataccact
      961 ggtactcaag aaaaaataac taaagacaaa gatattgtgt tcacaaatgg gggaaatgtc
     1021 ctgtttaagg ataatttgga ttttggtagc ggtggtatta tctttgacga aggccatgaa
     1081 tataacataa acggtcaggg atttacattt aaaggagcag gaattgatat cggaaaagaa
     1141 agcattgtaa actggaatgc attgtattcc agtgatgatg ttttacacaa aataggcccc
     1201 ggtactctga atgttcaaaa aaaacagggg gcaaatataa agataggtga aggaaatgtt
     1261 attcttaatg aagaaggaac atttaacaat atataccttg caagcggaaa tggtaaggta
     1321 atactaaata aagataattc ccttggcaat gatcaatatg cggggatatt ttttactaaa
     1381 cgtggtggca cgctagattt aaatggacac aatcagactt ttactagaat tgccgccact
     1441 gacgatggaa caacaataac taactcagat acaacgaaag aagccgttct ggcaatcaat
     1501 aacgaagact cctacatata tcatgggaac ataaatggca at
//
LOCUS       DAHHTG010000294         1485 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.302, whole genome shotgun sequence.
ACCESSION   DAHHTG010000294 DAHHTG010000000
VERSION     DAHHTG010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1485
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.302"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            complement(119..1312)
                     /locus_tag="NBI74_004664"
     CDS             complement(119..1312)
                     /locus_tag="NBI74_004664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016243297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HCD1934862.1"
                     /translation="MKKIVLPVALALLGMNAGAYAADVSIDILSATVKDKRIEGASVT
                     LQRNGAQSVSGITNTSGSISLGTSFADNKDALLIVKKEGYSNLVVKCPCAGMTYAISP
                     VMTNLDGMRVVLSWGEKPFDLDSHLIFPGGHIYFDSKEGTDANLDVDDTDSYGPETVT
                     ISKKHFGESYIYAVQDYSNKGLPNSNYLSASKAKVFVYVGSSLVRSYSVPAGKRGNIW
                     TVFKLNPNGEFEDINSVTSANFNGTTLDVRDLATVIMPATDSSAPASPAMQNSGDTQL
                     ARKYNREGEAVYKTGQLEQAIQLFQQATELDGNYGQAFSNLGLAYQKNGNIAEAIWAN
                     RKAISLASGVNAATTRANSYYNIAKIYETSGQNAEALQHYQLAYTEKNKPSYEEAIAR
                     VKTKM"
BASE COUNT          411 a          351 c          288 g          435 t
ORIGIN      
        1 aacattaaaa atggttaata gtttgcgcct ctcacacagg tccaggcaac tgcgtaacag
       61 gcaaatcaaa ttttcgtaaa cagtgggcat catcagatgc ccacaaaata cagctgattt
      121 acattttcgt ctttacacgt gcaatagctt cttcataaga aggcttattt ttttccgtat
      181 acgccagttg ataatgctgc aatgcttcag cattctggcc cgaggtttca taaattttgg
      241 caatgttata ataggagttg gcgcgcgttg tcgccgcatt taccccactc gctaacgaga
      301 tcgctttacg atttgcccaa attgcttccg ctatgttgcc atttttctga tacgccagac
      361 ccaggttact aaatgcctgt ccgtaattgc cgtctaattc tgttgcctgt tggaagagtt
      421 gaatggcttg ctcaagttga ccggttttat aaacagcttc accttcgcga ttatattttc
      481 ttgccagttg agtatctcca ctgttttgca tcgcaggcga agccggagct gaagagtccg
      541 tcgcaggcat tatcacagtg gctaaatccc tgacgtctaa tgtggtgccg ttaaaattgg
      601 cgctggtaac agagttgata tcttcaaact cgccgtttgg attgagttta aacacggtcc
      661 agatgttccc tcgttttcct gcagggacac tataactacg taccagggag ctccctacat
      721 aaacaaacac ttttgcctta ctggcagaga gataattact gttgggcagc cctttattgc
      781 tatagtcctg cacggcatag atgtaacttt caccaaaatg ctttttgctg atagtgacag
      841 tttctggtcc atagctatcc gtatcatcaa catccagatt ggcatcagtt ccctctttac
      901 tatcaaaata gatatgtccg ccagggaaga tcaaatgcga atcaagatca aaaggctttt
      961 cgccccagct caaaactaca cgcatgccat ccagattcgt catcaccgga ctgatagcat
     1021 aagtcatgcc agcgcacgga catttgacca ccagattgga atacccctct tttttgacga
     1081 ttaatagagc atctttatta tcagcaaatg aagtccccaa actaatgctg cccgaagtat
     1141 ttgtgatacc agaaacagat tgtgcaccat tgcgttgcag cgtcaccgat gccccttcaa
     1201 tgcgtttatc tttcactgtc gcacttaaaa tatcaatgct tacatcggcg gcataagcac
     1261 ctgcattcat gcccaacagc gcgagagcaa ctggcaaaac aattttcttc atgtataaat
     1321 ccttttttta atgaatgtaa ggtggaaatt tcccagttat tattatcgtg ttaaaaggtg
     1381 aaaaaaaaga aataagtaca cttttcagat taacacccac tcaccttgaa acaaaaaggc
     1441 catccggaga tgacctgaac tttctgagaa acgctgtttt ttcat
//
LOCUS       DAHHTG010000295         1480 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.303, whole genome shotgun sequence.
ACCESSION   DAHHTG010000295 DAHHTG010000000
VERSION     DAHHTG010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1480)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1480)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1480
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.303"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            127..450
                     /locus_tag="NBI74_004665"
     CDS             127..450
                     /locus_tag="NBI74_004665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecR-like lipofamily protein"
                     /protein_id="HCD1934863.1"
                     /translation="MRLLILTLSLITLTGCTVTRQAHVSEVDAATGIVRLVYDQAFLQ
                     HARTDRYVSRGIADRACQQAGYTHAIPFGQPVGNCSLFAGSLCLNTEFTLSYQCHHSA
                     FPVFL"
     gene            622..1119
                     /gene="ftnA"
                     /locus_tag="NBI74_004666"
     CDS             622..1119
                     /gene="ftnA"
                     /locus_tag="NBI74_004666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin"
                     /protein_id="HCD1934864.1"
                     /translation="MLKPEMIEKLNEQMNLELYSSLLYQQMSAWCSYHTFEGAAAFLR
                     RHAQEEMTHMQRLFDYLTDTGNLPRINTVESPFAEYSSLDELFQETYKHEQLITQKIN
                     ELAHAAMTNQDYPTFNFLQWYVSEQHEEEKLFKSIIDKLSLAGKSGEGLYFIDKELST
                     LDTQN"
     gene            complement(1157..1396)
                     /locus_tag="NBI74_004667"
     CDS             complement(1157..1396)
                     /locus_tag="NBI74_004667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecH family protein"
                     /protein_id="HCD1934865.1"
                     /translation="MDSIHGHEVLNMMIESGEQYTHASLEAAIKARFGEQARFHTCSA
                     EGMTAGELVAFLAAKGKFIPSEEGFSTDQSKICRH"
BASE COUNT          391 a          363 c          304 g          422 t
ORIGIN      
        1 agcagccccc caatttctga cacatacaga tggcaagaaa tagcgcctgc caggcgtctt
       61 ttccggccat tgtcgcagcg ctgtaacgcg taaaatactg ctttctttta ctcttctggc
      121 tggaccatga gacttctgat tctgactctt tccttaataa cgctgacggg gtgtacggtt
      181 actcgccagg cccatgtgag cgaagttgac gcggcaacag gtattgttcg gttggtttat
      241 gatcaggctt ttttgcagca tgcccgtacc gatcgttatg tcagtcgcgg gatcgccgat
      301 cgcgcatgcc agcaggcagg ctatacccac gctatcccct ttggtcagcc agtaggcaac
      361 tgtagcctct ttgcgggttc cttatgtttg aatacggaat tcactttgtc atatcagtgc
      421 catcattccg ccttccctgt ctttctctaa acgttttaat cagcgagggg gatcttcgct
      481 gattaaataa atagcggtaa tgcgttttat tcccattcgt atttttaata aatgatgttt
      541 atattttacc ttttgcaaat aataaaataa caaattatag tgacgctaca gcaacaaata
      601 taacctttgt ggagcactat catgctgaaa ccagaaatga ttgaaaaact taatgagcag
      661 atgaacctgg aactgtactc ttcactgctt tatcagcaaa tgagcgcctg gtgcagctat
      721 cataccttcg aaggtgctgc cgcgttcctg cgccgtcacg cccaggaaga gatgacgcat
      781 atgcagcgtc tgtttgatta cctgactgat accggcaact taccgcgtat taataccgtt
      841 gaatctccgt ttgctgaata ttcctcactt gatgaattat tccaggaaac ctataaacac
      901 gaacaattaa tcacgcagaa aattaacgaa ctggctcatg ctgcaatgac caatcaggac
      961 tacccaacat ttaatttcct gcaatggtat gtttctgagc agcatgaaga agagaaactg
     1021 ttcaaatcga ttattgataa attaagcctg gcaggcaaaa gtggcgaagg tctgtatttt
     1081 atcgacaaag aactctctac cctcgacaca caaaactaat gctctccggc ggcaggtttt
     1141 ctgccgccgt ttcaaatcag tgacggcaaa tcttactctg atcggttgaa aaaccttctt
     1201 ctgaaggtat aaatttgcct tttgctgcca gaaacgccac caactctccc gcagtcatcc
     1261 cttctgccga gcaggtgtga aaacgtgcct gttcaccaaa acgcgcttta attgctgctt
     1321 ccagactggc atgcgtatat tgctcgcctg attcaatcat catatttaac acttcatgac
     1381 cgtgaataga gtccatcgtt cctcctcaaa aaaagcctag cgtagcgatt gctgcttatg
     1441 aagactttgc gccagcgcag aactgaacgc tttttattgt
//
LOCUS       DAHHTG010000296         1472 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.304, whole genome shotgun sequence.
ACCESSION   DAHHTG010000296 DAHHTG010000000
VERSION     DAHHTG010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1472)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1472)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1472
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.304"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            62..886
                     /locus_tag="NBI74_004668"
     CDS             62..886
                     /locus_tag="NBI74_004668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HCD1934866.1"
                     /translation="MPENENLLLRLRQSVDGYSRTQQKLGEFVLSDPAKVVYLTITEL
                     ARESGTSEASVTRLCRTLGCKGYNEFKMALALDLRQVQSVEYGGDEIDNVVNESVQAL
                     QDTAKLLDRTLLEAATLALHQAQSVQIYGVAASAILGEYLHYKLLRLGKPAQLFSDMH
                     RAAMNATTLSKNTFVVAISSSGSTRDLLHVVKLARKQGVRVLALSNTPRSPLASLSDI
                     QLVAAKPEGPLSAGALNAKVGVMLLVELLTTSLIALDHKYSDVSQQTASATLPLLL"
     gene            complement(1058..>1472)
                     /locus_tag="NBI74_004669"
     CDS             complement(1058..>1472)
                     /locus_tag="NBI74_004669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS1-like element IS1F family transposase"
                     /protein_id="HCD1934867.1"
                     /translation="IQPGSDVIVCAEMDEQWGYVGAKSRQRWLFYAYDRIRRTVVAHV
                     FGERTLATLERLLSLLSAFEVVVWMTDGWPLYESRLKGKLHVISKRYTQRIERHNLNL
                     RQHLARLGRKLLSFSKSVELHDKVIGHYLNIKHYQ"
BASE COUNT          380 a          383 c          369 g          340 t
ORIGIN      
        1 ctggcatctc tcccctactt tccgccacac tatcggcaga gaaacgcatt aaggattttg
       61 catgccagaa aatgaaaacc tgctgctgag attgcgccag agcgttgatg gatatagtcg
      121 aactcaacag aagctgggag agtttgtact tagcgatccg gcaaaagttg tctacctgac
      181 gattaccgaa ctggcgcgtg aaagcggcac cagcgaggct agcgtcacac gtctgtgccg
      241 aacgctgggg tgtaagggct ataacgaatt caaaatggcg cttgcactcg atctccgaca
      301 agtacagtct gtagagtacg gcggtgatga gattgacaat gtggtcaatg aatccgtgca
      361 agcactacag gacacagcaa aattactcga taggaccttg ctggaagcag ccacgcttgc
      421 actgcatcag gctcagtcag tacagattta tggcgtagca gccagtgcta ttctggggga
      481 gtatttacat tacaaactcc tgcgtctggg gaaacccgca caattgttca gcgatatgca
      541 tcgtgctgcc atgaacgcca caacacttag taaaaacacg ttcgtggtgg cgatttccag
      601 ttctggatcc acgcgcgatc tgctgcatgt cgtaaaacta gcgcgtaagc aaggtgtgcg
      661 tgtactggcg ctgagtaata cgccgcgcag tccgttagct tcgctgagtg atattcaact
      721 ggtcgcggcc aaaccggaag gccccttgag cgccggcgcg ctgaatgcca aggtgggagt
      781 tatgctgttg gtagagttac tcaccacatc gctgattgcg ctggatcata agtacagcga
      841 tgtgagccag caaactgcca gcgcgaccct accgctactg ctttaaagat ataaaaaacc
      901 gcaaaagcac tgagggatcc ccatgaatca gcgctaataa accaacacca tattatggtc
      961 ggttctggcg aagtgattaa gaacggtgat cagcaccatt cgttttaaaa ttagcgagga
     1021 gtatcgcaag atattcttcg ctggtaatga ctccaactta ctgatagtgt tttatgttca
     1081 gataatgccc gatgaccttg tcatgcagct ccaccgattt tgagaacgac agtaacttcc
     1141 gtcccagcct tgccagatgt tgtctcagat tcagattatg tcgctcaatg cgctgagtgt
     1201 aacgcttgct gataacgtgc agctttccct tcaggcgtga ttcatacagc ggccagccat
     1261 ccgtcatcca taccacgacc tcaaaggccg acagcaggct cagaagacgc tccagtgtgg
     1321 ccagagtgcg ttcaccgaag acgtgcgcca caaccgtcct ccgtatcctg tcatacgcgt
     1381 aaaacagcca gcgctgacgt gatttagcac cgacgtagcc ccactgttcg tccatttcag
     1441 cgcagacaat cacatcactg cccggttgta tg
//
LOCUS       DAHHTG010000297         1469 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.305, whole genome shotgun sequence.
ACCESSION   DAHHTG010000297 DAHHTG010000000
VERSION     DAHHTG010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1469)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1469)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1469
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.305"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            217..450
                     /gene="papI"
                     /locus_tag="NBI74_004670"
     CDS             217..450
                     /gene="papI"
                     /locus_tag="NBI74_004670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000006209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major pilus subunit operon transcriptional
                     regulator PapI"
                     /protein_id="HCD1934868.1"
                     /translation="MSEYMKNEILEFLNRHNGGKTAEIAEALAVTDYQARYYLLLLEK
                     EGVVQRSPLRRGMATYWLLKGEKQAGQNCSSTT"
     gene            complement(1101..>1469)
                     /locus_tag="NBI74_004671"
     CDS             complement(1101..>1469)
                     /locus_tag="NBI74_004671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HCD1934869.1"
                     /translation="FVYVAFIIDVFAGYIVGWWVSSSMETTFVLDALEQALWARRPSG
                     TIHHSDKGSQYVSLAYMERLKEAKLLASTGSTGDSYDNAMAESINGLYKAEVIHRKSW
                     KNRAEVELATLTWVDWYNNR"
BASE COUNT          425 a          352 c          301 g          391 t
ORIGIN      
        1 ttgaatttac agcataaaag atcgtctaaa tgcattttta cggactttct tttcacagaa
       61 aaataacaca caaaatgtga tacacatcaa ataaagccaa caaaaagcaa attcactaaa
      121 ttgcacaaat aaagcaaaca cagagaataa aaataccttt ccgttgataa cagagagcat
      181 gatttttatt attcgaagaa actttgttac agtgagatga gtgaatatat gaagaatgaa
      241 atactggaat ttctgaacag gcataatgga gggaaaaccg cagaaattgc ggaggcgctg
      301 gcggtaacgg attatcaggc acgctattac ctgttattac tggaaaaaga aggtgtggtt
      361 cagcgctctc cattaaggcg aggcatggca acatactggc ttctgaaagg agagaagcag
      421 gcggggcaga actgttcttc cacaacttaa taactcaatc tgtgggcatg cttcactgaa
      481 acagataaaa gttacatcag agtgcagatt caattttgca gcattcatca caaaatagaa
      541 atttagtatt ttatgtcaga atacttcaca tgataataaa aacctgtctg gatttactgc
      601 atggccagac attacaaaat acaaacgcca taaacttcat tcagccttct tgctgtccgg
      661 taacggaggc tgctttttcg ttacaccttt ccccggcagc atcagtagca gtaatttata
      721 gattctacga actgagactg tctgtccggg gagaacgtac acagcccgac acaatgtacc
      781 agtcaataat atccggtttc tgattccctg cctgacagac cactctctgg caactttgta
      841 ttcagttcat ctactactgg agacagattt ataaaatcca actccacgat ctttgaccgt
      901 ccgcatcccc tactctctgt gctgcggtca tcggttaatt ccagcgtccg tccgttacga
      961 tcagatatta ctgaaccgcc ccgggtttcc tggagagtgt tttatctgtg aactcaggct
     1021 gccagatcat tgtttccgat ggaagcataa taagcttttt ctgcttctgc cggaggagta
     1081 tggcccagcc ttcccagcaa tcatcgattg ttataccagt ccacccacgt gagtgtggcc
     1141 agttccactt ctgcacggtt tttccagctc ttacggtgta ttacctccgc tttgtaaaga
     1201 ccattgatgc tctccgccat cgcgttgtca tacgagtcgc ctgtactccc tgttgatgcc
     1261 agcagttttg cttcttttaa tcgctccata taggccagtg acacatactg agagccttta
     1321 tcgctgtgat ggatggtgcc ggacggacgg cgggcccaca acgcctgctc cagcgcatcc
     1381 agcacgaatg tcgtttccat agacgatgag acccaccacc ccacgatgta tccggcaaac
     1441 acatcaatga tgaacgccac atagacgaa
//
LOCUS       DAHHTG010000298         1409 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.306, whole genome shotgun sequence.
ACCESSION   DAHHTG010000298 DAHHTG010000000
VERSION     DAHHTG010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1409)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1409)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1409
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.306"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            118..1329
                     /gene="bluF"
                     /locus_tag="NBI74_004672"
     CDS             118..1329
                     /gene="bluF"
                     /locus_tag="NBI74_004672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="blue light-responsive regulator BluF"
                     /protein_id="HCD1934870.1"
                     /translation="MLTTLIYRSHIRDAEPVKKIEEMVSIANRRNMRSDVTGILLFNG
                     SHFFQLLEGPEEQVQMIYRAICQDPRHYNIVELLCDYAPARRFGKAGMELFDLRLHER
                     DDVLQAVFDKGTSKFQLTYDDRALQFFRTFVLATEQSTYFEIPAEDSWLFIADGSDKE
                     LDSCTLSSNINDHFAFHPIVDPLSRRIIAFEAIVQKNEDSPSAIAVGQRKEGEIYTAD
                     LKSKALAFAMAHALKLGDKMISINLLPMTLVNEPDAVSFLLDEIKANALVPEQIIVEF
                     TESEVISRFDEFAEAVKSLKAAGISVAIDHFGAGFAGLLLLSRFQPDRIKISQELITN
                     VHKSGPRQAIIQAIMKCCTSLEIQVSAMGVATPEEWMWLESAGIEMFQGDLFSKAKLN
                     GIPSVAWPEKK"
BASE COUNT          400 a          292 c          300 g          417 t
ORIGIN      
        1 cttttccact ttttatcaat gtaaagaaac ggttatgttt tggtattaaa agaaagacac
       61 aagaagatat gtctgttacc gtcttactct cgcctcgccc attaccctgg attgttaatg
      121 cttaccaccc ttatttatcg tagccatata cgtgacgccg aacctgtcaa aaaaatcgaa
      181 gaaatggttt cgatagcaaa tcgcaggaac atgcggtctg acgtaacagg gatcttactg
      241 tttaatggtt ctcatttttt ccagcttctg gaaggtccgg aagaacaggt tcaaatgata
      301 tatcgggcca tatgccagga tccacggcac tataatattg ttgaactgtt gtgcgattac
      361 gcgcctgctc gccgttttgg caaagcagga atggaattat ttgatttacg cctgcacgag
      421 cgagatgacg ttttacaggc cgtattcgac aaaggcacat caaaatttca gctaacttat
      481 gatgacagag cgctacaatt ttttcgtact tttgtccttg caactgaaca atcaacctat
      541 ttcgagattc ctgctgaaga ctcctggctt tttatcgctg acggatctga taaagaactt
      601 gattcttgta ccctttcatc aaatataaac gaccactttg cctttcatcc tattgtcgac
      661 cctttatcgc ggcggataat cgcttttgaa gccattgtgc aaaaaaatga agacagcccg
      721 tcagctatag cggttgggca gcgtaaagaa ggggaaatct acacagcgga tctcaaaagt
      781 aaggcgcttg cattcgcgat ggcacacgca cttaagctcg gtgataaaat gatttcaatc
      841 aatctattac ctatgaccct ggttaacgag cctgacgcag tctctttttt acttgatgaa
      901 ataaaggcca atgctctggt gcctgaacaa atcatcgttg aatttactga aagtgaagtc
      961 atatctcggt ttgatgagtt tgccgaagcg gttaaatcgc taaaggctgc cggtatcagt
     1021 gtagcaattg atcattttgg cgcaggtttt gctggtttgt tactcctgtc acgcttccag
     1081 cctgacagaa ttaaaatcag tcaggaattg attaccaacg ttcataaaag cggaccacgg
     1141 caggcaatta ttcaggcgat catgaaatgc tgtacatcac ttgaaattca agtctctgct
     1201 atgggcgtgg caacaccaga agagtggatg tggcttgaat ctgcaggaat tgagatgttt
     1261 cagggagatc tgttttccaa agctaaattg aatggtatcc cttcagttgc gtggccggag
     1321 aaaaaataat tttcagcaca ttatttcgca tgatttctgg aaatcatcgt gaaaatgaat
     1381 acgttatgtt ttaatttaat ccacattaa
//
LOCUS       DAHHTG010000299         1404 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.307, whole genome shotgun sequence.
ACCESSION   DAHHTG010000299 DAHHTG010000000
VERSION     DAHHTG010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1404)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1404)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1404
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.307"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            complement(1040..1375)
                     /locus_tag="NBI74_004673"
     CDS             complement(1040..1375)
                     /locus_tag="NBI74_004673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1934871.1"
                     /translation="MKRAILLVLIAAGLLAGCGEKTPKCSSDDAKNLVGDIARKTIEK
                     GMKLDKDVRISVENVRTISHESDLDVYQCAADLTFTKPDLQNSLPITYRIQKTDEGKG
                     PFYINVTGL"
BASE COUNT          390 a          284 c          316 g          414 t
ORIGIN      
        1 tgatacctta caaatcatag ttatagagaa taaagaaccg ccggtatcaa tcatgaataa
       61 agttcattac gaatggtttg cgggtgaaca tgcaatggat ggagagcgca gagcccttat
      121 cccgctgccc tcaaaaaata gtgctatttt aatgaagtaa cttaggatgg gtagccatca
      181 aatcgcagtt atacgattgg tggggtttgt aaaagcggta gtcttgatta ccactgcatt
      241 cattctacaa gcacatactt ccctaggttg ctgtagttat ccgattacac gccttttctg
      301 aaagcagcct cagggagccg gataaaaccc gttaaaagtg gcttgcctat actgctgcgc
      361 ggcgctgacg ctttagaggc cccaggattt aggcaaatca atgtagttag ctcagtagca
      421 ggtcatatct gggctataaa tggcattacg tgacactgaa agagcttccc agtggcatcg
      481 tatttggtga gccccaacct acaagacggg cggggatagc cgtgattggc acccacagca
      541 aggcgataaa gatgttcaac ggagcggctc agcaggtatg gggatggtag gatttagtct
      601 tcatatgcct gtggaattaa tcaaccctca gagcccgaca cacccggcat tgcttacggt
      661 ataagactga ttgagaaggt gtttgacgtt tttgtatccc agtctgtcgg taaagtaaac
      721 acagtgatca gtaactggta gtactggaag tgattgcggt cgtaatgctg attttgcacg
      781 ctacgacagg tatattctgt ttggtatctg tagaggttgt gatggcgctg accttgcaca
      841 tgccaggtac gttagactta ataataaagg gagtattagc aggagggatt ttttcggact
      901 cagttgtatt gtgtgtaacg agccaactaa atgtgataat agctataatg gtaaatatga
      961 tgatcttttt catagggttt ccttttggaa taaaacatga attatttcac ccaaaaggaa
     1021 cccggatgtt attcagtgat tataaacctg ttacgtttat ataaaacgga cctttaccct
     1081 catcagtttt ctgaatccgg taggtgattg gtagagaatt ttgcaaatct ggttttgtaa
     1141 acgttaaatc agcagcgcac tgataaacat ccagatctga ttcatggcta attgttctga
     1201 cattttctac agaaatacgc acatccttat ccagtttcat ccctttctcg attgtctttc
     1261 ttgcaatatc cccgaccaga ttttttgcat catcactact acattttggc gttttctcgc
     1321 cgcagccagc cagcaaacca gcagcaatta ataccagaag tattgctctt ttcatctgta
     1381 gttccttcgt aaattaatga tact
//
LOCUS       DAHHTG010000300         1390 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.308, whole genome shotgun sequence.
ACCESSION   DAHHTG010000300 DAHHTG010000000
VERSION     DAHHTG010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1390)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1390)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1390
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.308"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            <1..583
                     /locus_tag="NBI74_004674"
     CDS             <1..583
                     /locus_tag="NBI74_004674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001544930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCD1934872.1"
                     /translation="FLLNGLIPFFIFSSISKRSVGAIEANQGLFNYRPVKPIDTIIAR
                     ALLETLIYVAVYILLMLIVRMAGEYFEITNFLQLVATWSLLIILSCSIGLIFMVVGKT
                     FPEMQKVLPILLKPLYFISCIMFPLHSIPKQYWSYLLWNPLVHVVELSREAVMPGYIS
                     EGVSLNYLAMFTLITLFIGLALYRTREEAMLTS"
     gene            580..1251
                     /locus_tag="NBI74_004675"
     CDS             580..1251
                     /locus_tag="NBI74_004675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000590239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCD1934873.1"
                     /translation="MIKIENLTKSYRTPTGRHYVFKDLNIEIPSGKSVAFIGRNGAGK
                     STLLRMIGGIDRPDSGKIITNKTISWPVGLAGGFQGSLTGRENVKFVARLYAKQEELK
                     EKIEFVEEFAELGKYFDMPIKTYSSGMRSRLGFGLSMAFKFDYYIVDEVTAVGDARFK
                     EKCAHLFKERHKESSFLMVSHSLNSLKEFCDVAIVFKDDNAVGFFEDVQEGIEEYKKI
                     KVVQQ"
BASE COUNT          402 a          259 c          307 g          422 t
ORIGIN      
        1 gtttttactt aatggcctga ttcccttttt tatctttagc agtatcagca aacgttctgt
       61 cggcgctatt gaagcgaacc aggggctgtt taattatcgt ccagtaaaac ccatcgatac
      121 gatcattgca cgtgcactgc ttgagacact gatttacgtt gctgtttata ttctgcttat
      181 gctcattgtc aggatggcag gcgaatattt cgagataaca aattttttac aacttgtggc
      241 tacctggagt ctgctgatca tactttcatg cagcattggc ttaatattca tggtcgttgg
      301 taaaaccttc cccgaaatgc aaaaggttct accgatactt cttaagcctc tttatttcat
      361 ctcctgcatc atgttccctc tgcactcaat tcctaagcaa tattggtctt atctactctg
      421 gaacccatta gtacatgtgg tggagctaag ccgcgaggcc gttatgcctg gctatataag
      481 cgaaggtgtg agtctgaact acctggcaat gttcacattg ataaccctgt tcattggtct
      541 ggcgttatac cgaactcgtg aggaggcaat gctgacatca tgattaagat tgagaatttg
      601 acgaagtcat atcgcacgcc aacggggcga cactatgtgt ttaaggattt aaacatcgaa
      661 atcccttcag gaaaaagcgt tgcctttatt ggtcgcaacg gcgcaggtaa atcaacgttg
      721 ctgagaatga ttggcggcat cgaccgcccc gatagcggaa agatcatcac caataaaacg
      781 atatcatggc cagtcggcct cgccggtgga ttccagggaa gtttaaccgg acgcgaaaac
      841 gtaaaatttg ttgcgcggtt atacgcgaag caagaagaac tgaaagagaa aatagagttt
      901 gttgaagaat ttgccgaact cggtaagtac tttgatatgc cgatcaaaac ttactcatct
      961 ggtatgcgat cgcgtttagg ctttggttta agcatggcct ttaagtttga ttattatatc
     1021 gtcgatgaag taaccgcagt cggtgatgcc aggtttaaag agaagtgcgc ccacttattt
     1081 aaagaaaggc ataaagagtc tagcttttta atggtttcac atagtttgaa ttcattgaaa
     1141 gagttttgtg atgtggcgat tgtgtttaaa gacgataatg cggttgggtt ttttgaggat
     1201 gttcaggagg ggatagagga gtataaaaaa ataaaggttg tacaacaata aacctaaata
     1261 gacctttaat tactgtgtgt tattaaaata aatctttttc cttttatatg atgcgtacat
     1321 aatccgtact taaataaaga agagaataga ctataagtca tgatttgaat tataaactcc
     1381 actataaata
//
LOCUS       DAHHTG010000301         1353 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.309, whole genome shotgun sequence.
ACCESSION   DAHHTG010000301 DAHHTG010000000
VERSION     DAHHTG010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1353)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1353)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1353
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.309"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            complement(<1..340)
                     /locus_tag="NBI74_004676"
     CDS             complement(<1..340)
                     /locus_tag="NBI74_004676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1398 family protein"
                     /protein_id="HCD1934874.1"
                     /translation="MDQVVVFQKMFEQVRKEQNFSWFYSELKHHRIAHYIYYLATDNI
                     RIITHDDTVLLLRGTRNLLKVSTTKNPAKIKEAALLHIRGKSTFREYCSTLAGAGVFR
                     WVTDVNHNKRS"
     gene            complement(561..1292)
                     /gene="bluR"
                     /locus_tag="NBI74_004677"
     CDS             complement(561..1292)
                     /gene="bluR"
                     /locus_tag="NBI74_004677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor BluR"
                     /protein_id="HCD1934875.1"
                     /translation="MAYYSIGDVAERCGINPVTLRAWQRRYGLLKPQRSEGGHRLFDE
                     EDIQRIEEIKRWISNGVPVGKVKALLETTRQDTQDDWSRLQEEMMSILRMANPAKLRA
                     RIISLGREYPVDQLINHVYLPVRQRLVLDHNTSRIMSSMFDGALIEYAAASLFEMRRK
                     PGKEAILMAWNVEERARLWLEAWRLSLSGWHISVLADPIESPRPELFPTQTLIVWTGM
                     APTRRQNELLQHWGEQGYKVIFHAP"
BASE COUNT          346 a          316 c          302 g          389 t
ORIGIN      
        1 aactgcgttt gttatgatta acatcagtaa cccaccggaa aacgcctgcg cctgccagtg
       61 ttgaacagta ttcccgaaat gtagattttc cgcgaatatg aagcaatgcg gcctctttta
      121 ttttagctgg gttcttggtc gtactaactt ttaacaggtt cctggttcct cttaataaca
      181 aaaccgtgtc atcgtgagta ataattctga tgttatccgt agccagataa taaatgtaat
      241 gtgcaatacg gtgatgtttt aattctgaat aaaaccagga gaagttttgc tcttttctca
      301 cttgctcaaa catcttttga aaaacaacga cctgatccat caggataata acctcttgtt
      361 aggtgtgaga ctgcgtagtg tgcacgatcg gttttaccac ttcaatctgg tctgtccttt
      421 ggctgtgata tgtacaaagt gtcaaagggg gattacctga attctcccgg tgagcatttt
      481 gcaacggacc agctccgcta caaacgctgt tgtgggttca gattataaca ttctgtctaa
      541 ggggcgggat aaaggtgaat ttagggggca tgaaagatga ctttataacc ttgctcaccc
      601 cagtgttgta aaagttcgtt ttgccttctc gttggtgcca tgcctgtcca gacaatcaat
      661 gtttgcgttg ggaacagttc cgggcgcggc gattcaatgg gatcagcaag aacagaaatg
      721 tgccatcctg acaatgataa acgccatgct tccagccaca atcgtgctct ctcttcaaca
      781 ttccacgcca tcagaatggc ctctttaccg ggcttacggc gcatttcgaa aagtgaggcc
      841 gctgcgtatt caattaatgc gccgtcaaac atactgctca taatgcggga ggtgttgtga
      901 tcaagcacga gacgctggcg aacaggaagg taaacatgat taatcaattg atctactggg
      961 tactctcgac ccagtgaaat aattcgcgcg cgtagtttgg ctggattagc catacgaaga
     1021 attgacatca tctcttcttg caggcggctc cagtcatctt gcgtatcctg gcgggtagtt
     1081 tccagtaatg ctttaacttt gcctacagga acgccattac ttatccaacg cttgatctct
     1141 tcgatgcgtt gtatgtcttc ttcatcaaag agtcggtgtc cgccttcact gcgttgtggt
     1201 tttaacaaac cgtagcggcg ttgccaggcc cggagagtga caggattaat cccgcaacgt
     1261 tcagcaacat caccaatgct gtaataagcc acaattcctc cttgcggtca taaatctccg
     1321 tcgcctctac acgacccaat aatactttgt aca
//
LOCUS       DAHHTG010000302         1344 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.310, whole genome shotgun sequence.
ACCESSION   DAHHTG010000302 DAHHTG010000000
VERSION     DAHHTG010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1344)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1344)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1344
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.310"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            20..238
                     /gene="ccdA"
                     /locus_tag="NBI74_004678"
     CDS             20..238
                     /gene="ccdA"
                     /locus_tag="NBI74_004678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin CcdA"
                     /protein_id="HCD1934876.1"
                     /translation="MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERW
                     KAENQEGMAEVARFIEMNGSFADENRDW"
     gene            240..545
                     /gene="ccdB"
                     /locus_tag="NBI74_004679"
     CDS             240..545
                     /gene="ccdB"
                     /locus_tag="NBI74_004679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin CcdB"
                     /protein_id="HCD1934877.1"
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSD
                     KVSRELYPVVHVGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     gene            546..>1344
                     /locus_tag="NBI74_004680"
     CDS             546..>1344
                     /locus_tag="NBI74_004680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase family protein"
                     /protein_id="HCD1934878.1"
                     /translation="MSGSVIHSQSAARVPAVYSAGQSPQLPVVIDYPAALALRQMSMV
                     HDELPKYLLAPEVSALLHYVPDLRRKMLLATLWNTGARINEALALTRGDFSLAPPYPF
                     VQLATLKQRTEKAARTAGRTPAGQQTHRLVPLSDSWYVSQLQTMVATLKIPLERRNKR
                     TGRTEKARIWEVTDRTVRTWIGEAVAAAATDGVTFSVPVTPHTFRHSYAMHMLYAGIP
                     LKVLQSLMGHKSISSTEVYTKVFALDVAARHRVQFSMPESDAVTMLKN"
BASE COUNT          314 a          344 c          393 g          293 t
ORIGIN      
        1 tgtcaaaaag aggtgtgcta tgaagcagcg tattacagtg acagttgaca gcgacagcta
       61 tcaattgctc aaggcatatg atgtcaatat ctccggtctg gtaagcacaa ccatgcagaa
      121 tgaagcccgt cgtctgcgtg ccgaacgctg gaaagcggaa aatcaggaag ggatggctga
      181 ggtcgcccgg tttattgaaa tgaacggctc ttttgccgat gagaacaggg actggtgaaa
      241 tgcagtttaa ggtttacacc tataaaagag agagccgtta tcgtctgttt gtggatgttc
      301 agagtgacat tattgacacg cccgggcgac ggatggtgat ccccctggcc agtgcccgcc
      361 tgctgtcaga caaagtctcc cgtgagcttt acccggtggt gcatgtcggg gatgaaagct
      421 ggcgcatgat gaccaccgat atggccagtg tgccggtctc cgttatcggg gaagaagtgg
      481 ctgatctcag tcaccgcgaa aatgacatca aaaacgccat taacctgatg ttctggggaa
      541 tataaatgtc aggctccgtt atacacagcc agtctgcagc cagggtaccg gcagtttatt
      601 ctgccggaca gtctccacaa cttcctgttg tcattgatta tccggcagct ctggcacttc
      661 gccagatgtc gatggttcat gatgaactgc cgaaatatct gctggctccg gaagtgagtg
      721 ccctgctcca ttacgtcccg gatctgcgcc gcaagatgct gctggccaca ctgtggaaca
      781 ccggtgcgcg cattaatgaa gcactggcgc tgacgcgggg ggatttttcg ctcgcgcctc
      841 cgtatccgtt tgtgcagctg gccactctga agcagcggac cgaaaaagcc gccaggacgg
      901 caggaagaac gcctgccgga cagcagactc accggctggt tccgctctcc gactcctggt
      961 acgtcagtca gctgcagacg atggtggcaa cactgaaaat tcctctggaa cggcgtaata
     1021 aacgaacagg caggacagag aaagcgcgga tctgggaagt gacggacaga acggtcagga
     1081 cctggattgg ggaggcggtt gccgccgctg ctactgacgg tgtgacgttc tctgtcccgg
     1141 tcacgccaca tacgttccgc cattcctatg cgatgcacat gctgtatgcc ggtataccac
     1201 tgaaggtcct gcagagcctg atggggcata agtccatcag ctcaacggag gtctacacga
     1261 aggtgtttgc gctggatgtg gctgcacggc accgggtgca gttttcgatg cctgagtccg
     1321 atgcggtcac aatgctgaaa aaca
//
LOCUS       DAHHTG010000303         1343 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.311, whole genome shotgun sequence.
ACCESSION   DAHHTG010000303 DAHHTG010000000
VERSION     DAHHTG010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1343)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1343)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1343
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.311"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            complement(<1..144)
                     /locus_tag="NBI74_004681"
     CDS             complement(<1..144)
                     /locus_tag="NBI74_004681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding protein"
                     /protein_id="HCD1934879.1"
                     /translation="MYDYIIVGSGLFGAVCANELKKLNKKVLVIEKRNHIGGNAYTED
                     CEGI"
     gene            complement(152..>1343)
                     /gene="wzx"
                     /locus_tag="NBI74_004682"
     CDS             complement(152..>1343)
                     /gene="wzx"
                     /locus_tag="NBI74_004682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="O16 family O-antigen flippase"
                     /protein_id="HCD1934880.1"
                     /translation="GSNYLLPLLTFPYLVRTLGPENFGIFGFCQATMLYMIMFVEYGF
                     NLTATQSIAKAADSKDKVTSIFWAVIFSKIVLIVITLIFLTSMTLLVPEYNKHAVIIW
                     SFVPALVGNLIYPIWLFQGKEKMKWLTLSSILSRLAIIPLTFIFVNTKSDIAIAGFIQ
                     SSANLVAGIIALAIVVHEGWIGKVTLSLHNVRRSLADGFHVFISTSAISLYSTGIVII
                     LGFISGPTSVGNFNAANTIRNALQGLLNPITQAIYPRISSTLVLNRVKGVILIKKSLT
                     CLSLIGGAFSLILLLGASILVKISIGPGYDNAVIVLMIISPLPFLISLSNVYGIQVML
                     THNYKKEFSKILIAAGLLSLLLIFPLTTLFKEIGAAITLLATECLVTSLMLMFVRNNK
                     LLVC"
BASE COUNT          480 a          270 c          211 g          382 t
ORIGIN      
        1 gataccctca cagtcctctg tgtacgcatt tccaccgata tgatttcttt tctcaatcac
       61 taaaactttt ttgtttagct tttttaactc attcgcacaa acggcaccaa acaaaccaga
      121 accaacaatg atataatcgt acataaaatc ctcagcaaac cagtaattta ttatttctta
      181 cgaacatcag catgagtgac gtaactaagc actctgttgc aagcaatgtt attgctgcac
      241 caatctcttt aaaaagagtt gttagcggaa aaatcaacaa caaactcaac aaacccgcag
      301 cgattaaaat cttactgaat tctttcttat aattatgggt cagcataact tgaatgccat
      361 agacattact taatgaaata agaaaaggca gaggcgatat aatcattagc acaatcactg
      421 cattatcata tcccggccct atacttattt ttactagtat agatgcaccc aagagcagaa
      481 ttaatgaaaa agcaccacca atcaaactca agcaggtcaa tgatttttta attaaaatca
      541 cacccttcac acgattaaga acaagcgtac ttgatattct tgggtatatt gcttgggtga
      601 taggatttaa tagcccttga agcgcgtttc ttatagtatt ggccgcatta aaattcccta
      661 cggacgttgg tccagatata aatcccagga taataactat tcccgtagaa tataaactaa
      721 tagcagatgt ggaaataaaa acatgaaaac cgtctgctaa agatcgacgc acattatgta
      781 atgatagcgt aactttacca atccaacctt catgaacaac gatagctagt gcaataattc
      841 cagcaaccag atttgcactt gactgaataa aaccggcaat tgctatatct gactttgtgt
      901 tcacaaaaat aaatgttaga gggataatag ccaagcggga taaaatacta cttaaagtca
      961 gccatttcat tttttctttt ccctgaaaca gccagatagg gtagattaaa ttcccgacta
     1021 atgcaggaac aaacgaccat ataattacgg catgcttgtt atattcagga acaagcaagg
     1081 tcatcgacgt taagaaaatc aatgtaatga cgataagaac tatttttgaa aatatcaccg
     1141 cccaaaaaat agacgttact ttatctttac tatctgctgc tttggcaata ctctgagttg
     1201 ctgtgagatt gaaaccatat tcaacaaaca ttatcatata tagcatagtc gcttggcaaa
     1261 aaccgaatat accgaaattt tcaggaccaa gtgttcttac aagatatgga aatgtaagca
     1321 atggtaaaag ataattgcta cct
//
LOCUS       DAHHTG010000304         1340 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.312, whole genome shotgun sequence.
ACCESSION   DAHHTG010000304 DAHHTG010000000
VERSION     DAHHTG010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1340)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1340)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1340
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.312"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            <1..956
                     /gene="ldtC"
                     /locus_tag="NBI74_004683"
     CDS             <1..956
                     /gene="ldtC"
                     /locus_tag="NBI74_004683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309518.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="L,D-transpeptidase LdtC"
                     /protein_id="HCD1934881.1"
                     /translation="KTRFSRWLTFFTFAAAVALALPAKANTWPLPSAGSRLVGENKFH
                     VVENDGGSLEAIAKKYNVGFLALLQANPGVDPYVPRAGSVLTIPLQTLLPDAPREGIV
                     INIAELRLYYYPPGKNSVTVYPIGIGQLGGDTLTPTMVTTVSDKRANPTWTPTANIRA
                     RYKAQGIELPAVVPAGPDNPMGHHAIRLAAYGGVYLLHGTNADFGIGMRVSSGCIRLR
                     DDDIKTLFSQVTPGTKVNIINTPIKVSAEPNGARLVEVHQPLSEKIDDDPQLLPITLN
                     SAMQSFKDAAQTDAEVMQHVMDVRSGMPVDVRRHQVSPQTL"
     gene            complement(1039..1296)
                     /gene="bhsA"
                     /locus_tag="NBI74_004684"
     CDS             complement(1039..1296)
                     /gene="bhsA"
                     /locus_tag="NBI74_004684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple stress resistance protein BhsA"
                     /protein_id="HCD1934882.1"
                     /translation="MKNVKTLIAAAILSSMSFASFAAVEVQSTPEGQQKVGTISANAG
                     TNLGSLEEQLAQKADEMGAKSFRITSVTGPNTLHGTAVIYK"
BASE COUNT          330 a          342 c          343 g          325 t
ORIGIN      
        1 tcaaaacgcg tttttctcgc tggctaacat tttttacgtt cgccgctgcc gtggcgctgg
       61 cgctaccggc aaaagccaac acctggccgc tgccgtcagc aggcagtcgt ctggttggtg
      121 aaaacaaatt tcatgttgtg gaaaatgacg gtggttctct ggaagccatc gccaaaaaat
      181 ataacgtcgg ctttctcgct ctgttacagg ctaaccccgg cgttgatcct tacgtaccgc
      241 gcgcgggtag cgtgttaacg atcccgttgc aaaccctact tccagacgcg ccgcgcgaag
      301 gcattgtgat caacattgcg gagctgcgtc tctattatta tccgccggga aaaaattcgg
      361 taaccgtata tcccatcggc attggtcaat taggtggtga cacgctgaca ccgacaatgg
      421 tgaccaccgt ttctgacaag cgtgcaaacc caacctggac gccaacggca aacattcgcg
      481 cgcggtataa ggcacaggga attgagttgc ctgcggtagt tccggctgga ccggataacc
      541 caatgggcca tcatgcgatt cgtctggcgg cctatggcgg cgtttatttg cttcacggta
      601 cgaacgctga tttcggcatt ggcatgcggg taagttctgg ctgtattcgt ctgcgggatg
      661 acgatatcaa aacactcttt agccaggtca ccccaggcac caaagtgaat atcatcaaca
      721 ccccgattaa agtctctgcc gaaccaaacg gtgcgcgtct ggttgaagta catcagccgc
      781 tgtcagagaa gattgatgac gatccgcagc tgctgccaat tacgctgaat agcgcaatgc
      841 aatcatttaa agatgcagca caaactgacg ctgaagtgat gcaacatgtg atggatgtcc
      901 gttccgggat gccggtggat gtccgccgtc atcaagtgag cccacaaact ctgtaaagct
      961 cggataaaaa aatccccgcg acacggcatg tcgcggggaa aaaaacaagc agtagcaggc
     1021 attaatgagg gttaatgctt atttataaat tactgctgtt ccatggaggg tattcggacc
     1081 ggttacagaa gtaatacgga aagattttgc gcccatctca tccgcttttt gcgccagctg
     1141 ctcttccagc gatcccagat ttgtccccgc gttagcactg atagtaccga ctttttgttg
     1201 gccttctggc gttgactgaa cttcgacagc cgcaaagctg gcaaatgaca tggagcttaa
     1261 aatcgccgca gcgatgaggg tttttacgtt tttcataata gtggccttat gcagatgaat
     1321 gacgtcgcaa gcaattactt
//
LOCUS       DAHHTG010000305         1316 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.313, whole genome shotgun sequence.
ACCESSION   DAHHTG010000305 DAHHTG010000000
VERSION     DAHHTG010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1316)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1316)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1316
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.313"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            <1..117
                     /locus_tag="NBI74_004685"
     CDS             <1..117
                     /locus_tag="NBI74_004685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCD1934883.1"
                     /translation="RRIEYTAASLDELNRAINDAELVLGTTRRRRRPLGVRL"
     gene            114..>1316
                     /locus_tag="NBI74_004686"
     CDS             114..>1316
                     /locus_tag="NBI74_004686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HCD1934884.1"
                     /translation="MKRTPVLIDVNGVPLRESLSYNGGGAGFGGQMAEWLPPAQSADA
                     ALLPALRLGNARADDLVRNNGIAANAVALHKDHIVGHMFLISYRPNWRWLGMRETATK
                     SFVDEVEAAWSEYAEGVFGEIDVEGKRTFTEFIREGVGVHAFNGEIFVQPVWDPESTQ
                     LFRTRFKAVSPKRVDTPGHGIGNRFLRAGVEVDRYGRAVAYHICEDDFPFSGSGRWER
                     IPRELPTGRPAMLHIFEPVEDGQTRGANQFYSVMERLKMLDSLQATQLQSAIVKAMYA
                     ATIESDLDTEKAFEYIAGAPQGQKDNPLINILDKFSTWYDTNSVTLGGVKIPHLFPGD
                     DLKLQTAQDSDNGFSALEQALLRYIAAGLGVSYEQLSRDYSKVSYSSARASANESWRY
                     FMGRRKFIA"
BASE COUNT          276 a          311 c          427 g          302 t
ORIGIN      
        1 cgaagaattg aatatacggc ggcctctctg gatgagctta accgtgcgat caatgatgct
       61 gagttggtac tggggacaac ccgccgtcgc cgtcgtccgc tgggagtgag gttatgaaac
      121 gaacgcctgt cctgattgat gtgaacggcg ttccgcttcg ggagagcctc agctacaacg
      181 ggggcggcgc aggatttggc gggcaaatgg cggagtggtt gccaccggcg cagagtgccg
      241 atgcggccct gctgcccgcg ttgcgtctgg ggaatgcccg ggcagatgat ctggtgcgca
      301 ataacgggat agcggccaat gcggtggccc tgcataagga tcacattgtc gggcatatgt
      361 ttcttatcag ctaccgcccg aactggcgct ggttggggat gcgggagacc gcgacaaaaa
      421 gttttgtcga tgaggtggag gcggcctggt cagaatacgc agaaggggtg tttggcgaga
      481 tcgacgtgga agggaaacgc acgtttacag aatttattcg tgaaggtgtg ggcgttcatg
      541 cgtttaacgg cgaaatcttt gtgcagccgg tctgggatcc ggagagcacg caactgtttc
      601 gtacgcgttt taaagccgtg agtccgaaac gggtggacac gccaggacat ggtatcggga
      661 accgttttct gcgggccggg gtggaggtcg atcgatatgg tcgtgccgtt gcgtaccata
      721 tctgtgagga tgattttccg ttctccggga gtggacgatg ggaacggatc ccgcgtgaac
      781 ttcccaccgg gcgtccggcc atgctgcata ttttcgagcc ggtggaggac gggcagaccc
      841 gtggggctaa tcagttttac agcgttatgg aacggctgaa gatgctcgat tccctgcagg
      901 caacacagct tcagtcggcc atagtgaagg cgatgtatgc agcgacgatt gaaagtgacc
      961 ttgataccga aaaggccttt gaatatatcg ccggtgcgcc gcaggggcag aaggataatc
     1021 cgcttattaa tattctggat aagttctcca cctggtatga cacgaatagc gtgacgctgg
     1081 gcggtgtcaa aattccgcac cttttccccg gtgatgatct gaaacttcag accgcgcagg
     1141 attcagacaa tggattttcg gcgcttgaac aggcgctgct gcggtatatc gccgccggtc
     1201 ttggcgtttc ctacgaacag ttgtcccgtg attactcgaa ggtcagttat tcaagtgccc
     1261 gcgcatccgc caatgagtcg tggcgctatt ttatggggcg gcgaaaattt attgcg
//
LOCUS       DAHHTG010000306         1288 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.314, whole genome shotgun sequence.
ACCESSION   DAHHTG010000306 DAHHTG010000000
VERSION     DAHHTG010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1288)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1288)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1288
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.314"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            <1..428
                     /locus_tag="NBI74_004687"
     CDS             <1..428
                     /locus_tag="NBI74_004687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HCD1934885.1"
                     /translation="GAKKICRLTFEQIKVIYQEMKLHFENYRVIFTGLPQDLLTIPIP
                     EIETLPFNEFIYTVALTKYSDFVISVDTALVHIAAAYHKPTLAFYPNSRTPEYPSHLI
                     WSPNNHKSIQIVSPTFTVKDIDTETLTDSVKRLSCIDKK"
     gene            complement(454..>1288)
                     /gene="waaL"
                     /locus_tag="NBI74_004688"
                     /gene_synonym="rfaL"
     CDS             complement(454..>1288)
                     /gene="waaL"
                     /locus_tag="NBI74_004688"
                     /gene_synonym="rfaL"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001141924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="O-antigen ligase"
                     /protein_id="HCD1934886.1"
                     /translation="LIAGYAMYINSIHENDRISFGVGTATGAAYSTMLIGIVSGVAIL
                     YTKKNHPFLFLLNSCAVLYVLALTQTRATLLLFPIICVAALIAYYNKSPKKFTSSIVL
                     LIAILASIVIIFNKPIQNRYNEALNDLNSYTNANSVTSLGARLAMYEIGLNIFIKSPF
                     SFRSAESRAENMNLLVAEHNRLRGALEFSNVHLHNEIIEAGSLKGLMGIISILFLYFS
                     LFYTAYKRRALGLLILTLGIVGIGLSDVIIWARSIPIIVISAIVLLLVINNRNNTINQ
                     E"
BASE COUNT          436 a          230 c          213 g          409 t
ORIGIN      
        1 taggtgcaaa aaaaatatgc cgacttacgt ttgagcaaat aaaagttata tatcaagaaa
       61 tgaaactaca ctttgaaaat tatcgggtga tatttaccgg gttaccgcaa gatttattga
      121 caataccaat acctgaaatc gaaacactac catttaatga gtttatttat accgttgcgt
      181 tgaccaagta tagtgatttt gtcatttctg ttgatacagc tcttgttcat atcgctgcgg
      241 cttatcataa accaacgctt gcattttatc ctaattcacg tactccggaa tatccctcac
      301 atttaatttg gtccccaaat aatcataaat caatacaaat agtatcccca acatttacgg
      361 tgaaagatat cgatactgaa actttaactg attcagtaaa gagattaagt tgtatagata
      421 agaagtgagt tttaactcac ttcttaaact tgtttattct tgattaatag tattgttacg
      481 attattaata acgagtaaga ggactatagc ggatataacg ataattggaa tgctgcgtgc
      541 ccatatgatc acatcactga gtccaatccc cacaatgcca agcgttaata tcaacaaacc
      601 caaagctcgt cttttatatg ctgtataaaa cagtgaaaaa tagaggaaaa ggatggaaat
      661 aattcccatc agacctttca gtgaccctgc ttcaattatc tcattatgta gatgtacgtt
      721 agaaaactcc aatgcccctc ttagcctgtt gtgttctgca actaacaaat tcatattttc
      781 agcgcgtgac tctgctgatc taaatgaaaa aggtgacttt atgaatatat ttaaaccaat
      841 ttcgtacatt gccagtcttg cacctaggga agtcacgcta ttagcattgg tataactgtt
      901 taaatcattt aatgcttcat tatagcgatt ctgtattggt ttattaaata taattactat
      961 gctagctaat atagcaatta gtagaacaat agaggaagtg aatttcttgg gtgatttatt
     1021 ataataagct attaatgcag caacacaaat tatagggaac aggagtaggg ttgctctggt
     1081 ttgtgttagc gccagaacat aaagtaccgc gcaactattt aataaaaata aaaaaggatg
     1141 gtttttctta gtataaagaa tcgcaacgcc actaactatc cctattagca tagttgaata
     1201 tgctgctcct gttgccgttc ctacaccaaa agaaatgcgg tcattttcat gaatgctatt
     1261 aatatacatt gcatatccag caattaga
//
LOCUS       DAHHTG010000307         1269 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.315, whole genome shotgun sequence.
ACCESSION   DAHHTG010000307 DAHHTG010000000
VERSION     DAHHTG010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1269)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1269)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1269
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.315"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            <1..1197
                     /locus_tag="NBI74_004689"
     CDS             <1..1197
                     /locus_tag="NBI74_004689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000512755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2345 domain-containing protein"
                     /protein_id="HCD1934887.1"
                     /translation="LPLLAGTEVSIAFEEGNPDRPYIAGVKHDSAHTDHVTIQNYKRN
                     VLRTPANNKIRLDDERGKEHIKVSTEYGGKSQLNLGHLVDAGKQQRGEGFELRTDLWG
                     AVRAKKGIFISADAQDKAQGKVREMAPAMAILDGAQSQMKSLSTDAQTANADPADLSS
                     QIALLQQSVKDLTQAAILLSAPKGVAIASGEHLQLAASKNLIANAGNHADIGVVKNMF
                     IGVGQALSVFVRKAGIKLFANKGAISVQAQNDLMELLAQKSIEITSTEDEIKITAKKK
                     ITLNGGGSYIRLDACGIEAGTPGEYNVKAGYYGRKPKAKLTPELMAFPVIKSEDFNQS
                     FILLDENTGQPLINWPYELELESGLKMSGITDENGNTELISSDKEEVVNISVFEPDEF
                     LDDEIN"
BASE COUNT          380 a          278 c          368 g          243 t
ORIGIN      
        1 ctgccgctgc tggcgggaac ggaagtcagc atcgcgtttg aggaaggcaa cccggaccga
       61 ccttatattg caggcgtaaa acacgactcg gcacacaccg accatgtgac cattcagaac
      121 tataagcgca acgtactgcg caccccggcg aacaacaaaa tccgcctcga tgacgagcgc
      181 ggcaaagagc atatcaaggt cagtaccgag tatggcggca agagccagct caacctcggc
      241 catctggtgg atgccggaaa acagcagcgc ggggaaggct ttgaactgcg taccgatctg
      301 tggggtgcag tacgggcaaa gaaaggcatc tttatcagcg ccgatgcgca ggacaaggcg
      361 cagggtaagg tgcgcgaaat ggctccggcg atggcaatac tggatggcgc acagtcacag
      421 atgaagtcgc tgtccactga tgcgcagacg gcgaatgccg atccggcaga tctgtcatca
      481 cagatagcgc tgttgcagca gagtgtaaaa gatctgacgc aggctgcgat cctgctaagc
      541 gcacccaaag gcgtggcgat cgccagcgga gagcacctgc aactggcggc gtcgaaaaac
      601 ctgatcgcga acgcaggaaa tcacgctgat atcggtgtcg tgaagaatat gtttatcggc
      661 gtgggtcagg cgctgagcgt gtttgtacgc aaagcgggta tcaaactgtt tgccaataaa
      721 ggggcgatct cggtacaggc acagaacgat ctgatggaac tgttggcgca gaaatcgata
      781 gagatcacca gtacggaaga tgagattaaa atcaccgcga agaagaaaat cacccttaac
      841 ggcggtggca gctacatccg gctggatgcc tgtgggatag aggccggtac gccgggagaa
      901 tataacgtta aggcgggata ttacgggcgt aagccgaagg cgaagctgac gccggagctg
      961 atggcatttc cggtgattaa gtctgaggat tttaatcaga gctttatttt gctggatgaa
     1021 aatactgggc aaccacttat taattggccc tatgaactag aactggaaag tgggctaaaa
     1081 atgagtggga ttactgatga aaatggaaat acagaactaa tatcctctga taaggaggag
     1141 gtcgttaata ttagtgtgtt tgaacccgat gagtttttag acgatgaaat taattaagac
     1201 atatagcgag aatataacat ggcagataaa tccgaaaaga aaaaagaaac cacaaataaa
     1261 tcagcaaaa
//
LOCUS       DAHHTG010000308         1253 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.316, whole genome shotgun sequence.
ACCESSION   DAHHTG010000308 DAHHTG010000000
VERSION     DAHHTG010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1253)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1253)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1253
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.316"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            129..632
                     /locus_tag="NBI74_004690"
     CDS             129..632
                     /locus_tag="NBI74_004690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin-like protein"
                     /protein_id="HCD1934888.1"
                     /translation="MATAGMLLKLNSQMNREFYASNLYLHLSNWCSEQSLNGTATFLR
                     AQAQSNVTQMMRMFNFMKSVGATPIVKAIDVPGEKLNSLEELFQKTMEEYEQRSSTLA
                     QLADEAKELNDDSTVNFLRDLEKEQQHDGLLLQTILDEVRSAKLAGMCSVQTDQHVLN
                     VVSHQLH"
     gene            complement(710..961)
                     /locus_tag="NBI74_004691"
     CDS             complement(710..961)
                     /locus_tag="NBI74_004691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2766 domain-containing protein"
                     /protein_id="HCD1934889.1"
                     /translation="MSQPLNADQELVSDVVACQLVIKQILDVLDVIAPVEVREKMSSQ
                     LKNIDFTNHPAAADPVTMRAIQKAIALIELKFAPQGESH"
     gene            complement(1076..1162)
                     /gene="azuC"
                     /locus_tag="NBI74_004692"
     CDS             complement(1076..1162)
                     /gene="azuC"
                     /locus_tag="NBI74_004692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein AzuC"
                     /protein_id="HCD1934890.1"
                     /translation="MKLRKILKSMFNNYCKTFKDVPPGNMFR"
BASE COUNT          336 a          289 c          280 g          348 t
ORIGIN      
        1 agatcatcgt cagaattgac tccacgatca catttcggac cggcagaaag gaattattct
       61 gcaaacagta attatggtgt tttgatttat cttgcacctc tccacttctg gatataagga
      121 tattaggtat ggcaaccgct ggaatgcttc tcaaactcaa ctctcaaatg aaccgcgagt
      181 tttacgcatc caatctctac cttcacctga gtaactggtg ttctgaacag agtctgaacg
      241 gcaccgccac tttccttcgc gcccaggcac agagtaatgt gacccaaatg atgcgcatgt
      301 ttaactttat gaagagtgtc ggcgctaccc ccatcgttaa agccattgat gttcccggtg
      361 aaaaactgaa ctctctggaa gaactgttcc aaaaaacgat ggaagaatac gagcaacgtt
      421 caagcacgct ggcgcagtta gccgatgaag cgaaagaact gaatgatgat tcaaccgtca
      481 atttcctgcg cgatctggaa aaagaacagc agcatgatgg cctgttactg caaaccattc
      541 ttgatgaagt gcgcagtgcg aaacttgctg gtatgtgttc tgtgcagacc gaccaacatg
      601 ttctgaatgt cgtgtcgcat cagctacatt gatcatcatc ggcgctaatg cattgcgccg
      661 atcatgtttt tgagaaactg ctgcctcatt tttttgaagc agcggttttt taatgggatt
      721 cgccctgtgg ggcaaacttg agttcaatca acgcgatggc tttttggatt gcccgcatgg
      781 tgaccgggtc tgcggcggcg ggatggttag taaagtcgat attcttcagc tgactggaca
      841 ttttttcgcg aacttcaacg ggcgcgatca catcgagaac atccagaatt tgtttgataa
      901 ccaactggca agcaaccaca tcagaaacca gttcctgatc ggcattcagc ggctgggaca
      961 tcgtaaactc ctgatagcat tttgaaagcc gttatagtag cggcttcaca tcttcagcga
     1021 tagtcacatc caccgtcatc agaacacaaa aaaacctgcc ggagcaggtt ttttgttatc
     1081 ggaacatatt gcctggcggt acgtctttga acgtcttgca atagttattg aacatacttt
     1141 tcaggatctt gcgcagtttc atcgcggtac tccgaccatt tgttatacag gtgttattgt
     1201 ctttgcgttt ataatatgac aaccatcaca aaaatcaatc tttatgtgat gta
//
LOCUS       DAHHTG010000309         1248 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.317, whole genome shotgun sequence.
ACCESSION   DAHHTG010000309 DAHHTG010000000
VERSION     DAHHTG010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1248)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1248)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1248
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.317"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            68..1150
                     /gene="ompC"
                     /locus_tag="NBI74_004693"
     CDS             68..1150
                     /gene="ompC"
                     /locus_tag="NBI74_004693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HCD1934891.1"
                     /translation="MKKLTVAISAVAASVLMAMSAQAAEIYNKDSNKLDLYGKVNAKH
                     YFSSNDADDGDTTYARLGFKGETQINDQLTGFGQWEYEFKGNRAESQGSSKDKTRLAF
                     AGLKFGDYGSIDYGRNYGVAYDIGAWTDVLPEFGGDTWTQTDVFMTGRTTGVATYRNN
                     DFFGLVDGLNFAAQYQGKNDRTDVTEVNGDGFGFSTTYEYEGFGVGATYAKSDRTDGQ
                     VAYGKSKFNASGKNAEVWAAGLKYDANNIYLATTYSETQNMTVFGNNHIANKAQNFEA
                     VAQYQFDFGLRPSVAYLQSKGKDLGVHGDRDLVKYVDVGATYYFNKNMSTFVDYKINL
                     IDDSKFTKTAGIDTDDIVAVGLVYQF"
BASE COUNT          363 a          260 c          277 g          348 t
ORIGIN      
        1 gattagatga atatttatcg cgcagtgaca tcatttttta ataatagttc aaaaaaaagg
       61 gcgtacaatg aaaaaattaa cagtggcaat ttctgctgta gctgcatcag tactgatggc
      121 gatgtctgct caggcagctg aaatttataa taaagacagt aacaagctgg atctatacgg
      181 gaaagttaat gccaagcact acttctcctc taatgatgca gatgatggtg atactactta
      241 tgcccgtctt ggcttcaaag gtgaaaccca aatcaacgat caactgactg gtttcggtca
      301 gtgggaatat gaattcaaag gcaaccgtgc tgaatctcaa ggttcttcca aagacaaaac
      361 ccgtcttgca tttgcaggcc tgaaatttgg tgattacggc tcaatcgatt acggccgtaa
      421 ctacggtgta gcatacgaca tcggtgcgtg gactgacgtc ctgccagaat tcggtggtga
      481 tacctggacc caaacagatg tgttcatgac tggtcgcact actggtgttg caacttatcg
      541 taacaacgac ttctttggtc tggtcgatgg cctgaacttt gctgctcagt atcagggtaa
      601 aaatgaccgc actgacgtaa ctgaagtcaa tggtgatggt ttcggtttct ccactactta
      661 tgagtatgaa ggattcggcg tgggtgcaac ctatgctaaa tcagatcgca ctgacggtca
      721 ggtcgcctat ggtaagagca aattcaatgc ctccggcaaa aatgcggaag tatgggctgc
      781 aggcctgaaa tatgatgcga acaatatcta tctggctacc acatattctg aaactcagaa
      841 tatgaccgtt tttggtaata accatattgc aaacaaagca caaaactttg aagcagtagc
      901 acaatatcag tttgacttcg gtctgcgacc atctgttgct taccttcagt caaaaggtaa
      961 agaccttggt gttcatggtg accgagactt agtcaagtat gtcgatgtcg gtgctactta
     1021 ctactttaat aaaaacatgt ccacttttgt tgattacaaa atcaacttaa ttgacgatag
     1081 taagtttacc aaaacagctg gtattgatac cgacgacatc gtcgctgtag gtctggttta
     1141 tcagttctaa tctgacttac gaaaaagata tgttgcggga ggctttgcct ccgcaacata
     1201 taagtggagc cctcaagcca cttcctttag aagcactacc ttgcttct
//
LOCUS       DAHHTG010000310         1239 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.318, whole genome shotgun sequence.
ACCESSION   DAHHTG010000310 DAHHTG010000000
VERSION     DAHHTG010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1239)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1239)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1239
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.318"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            <1..392
                     /locus_tag="NBI74_004694"
     CDS             <1..392
                     /locus_tag="NBI74_004694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HCD1934892.1"
                     /translation="ASWHLVEDHRGKTVYDVASGDALFISELGPLPENVTWLSPEGEF
                     QKWNGTAWVKDAEAEKLFRIREAEETKNSLMQVASEHIAPLQDAVDLEIATEEETSLL
                     EAWKKYRVLLNRVDTSTAQDIEWPALP"
     gene            complement(490..1080)
                     /locus_tag="NBI74_004695"
     CDS             complement(490..1080)
                     /locus_tag="NBI74_004695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCD1934893.1"
                     /translation="MSRIFAYCRISTLDQTIENQRREIESAGFKIKPQQIIEEHISGS
                     AATSERPGFNRLLARLKCGDQLIVTKLDRLGCNAMDIRKTVEQLTETGIRVHCLALGG
                     IDLTSPTGKMMMHVISAVAEFERDLLLERTHSGIVRARGAGKRFGRPPVLNEEQKQVV
                     FERIKSGVSISAIAREFKTSRQTILRAKAKLQTPDI"
BASE COUNT          326 a          256 c          297 g          360 t
ORIGIN      
        1 aggcatcgtg gcatctcgtt gaagaccatc ggggtaaaac ggtttatgac gtagcgtcag
       61 gggacgcgtt atttatttct gaactcggtc cgttaccgga aaatgttacc tggttatcgc
      121 cggaagggga gtttcagaag tggaacggca cagcctgggt gaaagatgca gaagcagaaa
      181 aactgttccg gatccgagag gcggaagaaa caaaaaacag cctgatgcag gtagccagtg
      241 agcatattgc gccacttcag gatgctgtag atctggaaat cgcaacggag gaagaaacct
      301 cattgctgga agcctggaaa aagtatcggg tgttgctgaa ccgtgttgat acgtcaactg
      361 cacaggatat tgaatggcca gcactgccgt agggtaaaac atataaattc tataattaga
      421 tgtatctttc catttacggc aaggaagggg gcttggaaga cgtaaagcat ctcacaccga
      481 gattattttt tatatgtcag gtgtctgaag ttttgctttg gctcttaaaa tggtttgccg
      541 cgaggttttg aattcccggg caatggcact tatacttaca cctgacttaa ttcgttcgaa
      601 taccacctgt ttctgttctt catttaacac aggtggtcga ccaaaacgtt tccctgcgcc
      661 gcgggctctt actatcccgg aatgagtgcg ttcaagtaaa aggtctcgtt caaattcagc
      721 gactgctgaa attacgtgca tcatcatttt tcctgttgga ctggtcaggt caatgccccc
      781 caatgctaag caatgcactc tgatacctgt ttcggtcagt tgttccactg ttttcctgat
      841 atccattgca ttacaaccaa ggcgatccag ttttgtcaca atcaattgat caccacattt
      901 caggcgagca agcaaccggt taaaaccagg acgctcactg gttgctgctg agccgctaat
      961 gtgttcttcg attatttgct gaggtttgat tttaaaacct gcactttcga tttcccggcg
     1021 ttgattttcg atggtctgat ccagcgttga tatccgacag taagcaaaaa ttcgagacat
     1081 agtgagactc tatacgaaat tggtgttcat atcataatgc atctcagaaa ataattttga
     1141 ttatttttgt gcatcggttt gtttttcgag ttccgggcgg actcaaggaa gaagaatagt
     1201 gttgcgtgtt attttaacca gatttcaagt tgtttgatc
//
LOCUS       DAHHTG010000311         1236 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.319, whole genome shotgun sequence.
ACCESSION   DAHHTG010000311 DAHHTG010000000
VERSION     DAHHTG010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1236)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1236)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1236
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.319"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            <1..487
                     /locus_tag="NBI74_004696"
     CDS             <1..487
                     /locus_tag="NBI74_004696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001625142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1934894.1"
                     /translation="SLEYANGERSEVEIATYLNVDFGVRQGKKIILYATNPPLSMKVP
                     YTMVQKLFGEDSGLRPVEFDLRKIVNGLSEGFDIKIKSMSLSNISVDPFTLAKTKIVS
                     SKNLQEIYQNKYMNSSAVFDSVHFFVNGIETELSRTGRFRVRESQLPTLLSILETLRI
                     L"
     gene            1100..>1236
                     /gene="hokD"
                     /locus_tag="NBI74_004697"
     CDS             1100..>1236
                     /gene="hokD"
                     /locus_tag="NBI74_004697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HCD1934895.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     TA"
BASE COUNT          361 a          236 c          282 g          357 t
ORIGIN      
        1 aagcttagaa tatgctaatg gcgagcgttc agaggttgaa atagcaactt acttaaatgt
       61 ggactttggc gtcagacaag ggaaaaaaat catactatat gccactaatc ctccattaag
      121 tatgaaagtg ccttatacta tggtgcaaaa actatttggt gaagatagtg gcttgagacc
      181 agtagaattt gatctgagaa aaattgtaaa tggtctttct gagggctttg acatcaagat
      241 aaaatccatg tctctatcta atatctctgt tgatcctttt actttagcaa agacaaaaat
      301 agtaagctca aagaatctcc aagaaattta tcagaataaa tacatgaata gttcggccgt
      361 ttttgattcc gtacatttct tcgttaacgg aattgaaacg gaattatcta ggacgggtag
      421 attccgtgtg agagagagtc agcttccaac acttttatcc atcttagaaa ctctcagaat
      481 tttgtgaaat atataaggaa ctcaggccta cttcgtcagt aggccccttt tccggtttgt
      541 acttatctag tcatcgattt gatcgtaatg attcacacag gatgctgtta gcaatgtccg
      601 ctcttcgctc aaagcggact agaaggttag cttgcgtcgg acttggcgta tttaaagaag
      661 tgctggtggt gactggttgt tgtgttccat ttctacagaa caaaatcaca gaaactatac
      721 ccaatagtta tattgaatca atgatgagac agcctcatat ttatcagaat tggtgtacgt
      781 ccaatacagg aggttgtcgt gctggttctc aaatgtgcgc tagctattgc ggctgtaatg
      841 gcaatttatt gtcttgctgt tgttcttacg gatcgccttt ctgattgatt ttatattggc
      901 gaggtgacgg gagttaagta gaattgctgc gggtgcttga ggctatctgc ctcaggcatg
      961 aacaccaaag gcagatagag aaaagcccca gttaacatta cgcgtcctgc aagacgttta
     1021 acattaatct gaggctcaat ctatgaacgg caaatctagg ttagcctctt acgtgccgaa
     1081 aggcaaggag aagcaggcta tgaagcagca aaaggcgatg ttaatcgccc tgatcgtcat
     1141 ctgtttaacc gtcatagtga cggcactggt aacgaggaaa gacctctgcg aggtacgaat
     1201 ccgaaccggc cagacggagg tcgctgtctt cacagc
//
LOCUS       DAHHTG010000312         1229 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.320, whole genome shotgun sequence.
ACCESSION   DAHHTG010000312 DAHHTG010000000
VERSION     DAHHTG010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1229)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1229)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1229
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.320"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            45..998
                     /gene="ompT"
                     /locus_tag="NBI74_004698"
     CDS             45..998
                     /gene="ompT"
                     /locus_tag="NBI74_004698"
                     /EC_number="3.4.23.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="omptin family outer membrane protease OmpT"
                     /protein_id="HCD1934896.1"
                     /translation="MRAKLLGIVLTTPIAISSFASTETLSFTPDNINADISLGTLSGK
                     TKERVYLAEEGGRKVSQLDWKFNNAAIIKGAINWDLMSQISIGAAGWTTLGSRGGNMV
                     DQDWMDSSNPGTWTDESRHPDTQLNYANEFDLNIKGWLLNEPNYRLGLMAGYQESRYS
                     FTARGGSYIYSSEEGFRDDIGSFPNGERAIGYKQRFKMPYIGLTGSYRYEDFELGGTF
                     KYSGWVEASDNDEHYDPGKRITYRSKVKDQNYYSVAVNAGYYVTPNAKVYIEGAWNRV
                     TNKKGNTSLYDHNDNTSDYSKNGAGIENYNFITTAGLKYTF"
BASE COUNT          392 a          261 c          253 g          323 t
ORIGIN      
        1 aaaatacata ctcaatcatt aaaacgattg aatggagacc ttttatgcgg gcgaaacttc
       61 tgggaatagt cctgacaacc cctattgcga tcagctcttt tgcttctacc gagactttat
      121 cgtttactcc tgacaacata aatgcggaca ttagtcttgg aactctgagc ggaaaaacaa
      181 aagagcgtgt ttatctggcc gaagaaggag gccgaaaagt cagtcaactt gactggaaat
      241 tcaataacgc tgcaattatt aaaggtgcaa ttaattggga tttgatgtcc cagatatcta
      301 tcggggctgc tggctggaca acactcggca gccgaggtgg caatatggtc gatcaggact
      361 ggatggattc cagtaacccc ggaacctgga cggatgaaag tagacaccct gatacacaac
      421 tcaattatgc caacgaattt gatctgaata tcaaaggctg gctcctcaac gaacccaatt
      481 accgcctggg actcatggcc ggatatcagg aaagccgtta tagctttaca gccagaggtg
      541 gttcctatat ctacagttct gaggagggat tcagagatga catcggctcc ttcccgaatg
      601 gagaaagagc aatcggctac aaacaacgtt ttaaaatgcc ctacattggc ttgactggaa
      661 gttatcgtta tgaagatttt gagctaggtg gcacatttaa atacagcggc tgggtggaag
      721 catctgataa cgatgagcac tatgacccag gaaaaagaat cacttatcgc agtaaagtca
      781 aagaccaaaa ttactattct gttgcagtca atgcaggtta ttacgtaaca cctaacgcaa
      841 aagtttatat tgaaggcgca tggaatcggg ttacgaataa aaaaggtaat acttcacttt
      901 atgatcacaa tgataacact tcagactaca gcaaaaatgg tgcaggcata gaaaactata
      961 acttcatcac tactgctggt cttaagtaca ccttttaaca acgttaaata gattttctcc
     1021 ccggtggtat tgtatagccc cggggaattt gtttaccgtt aaaagcaaac aatatcccct
     1081 aatcttgtaa gcatttaacc tgcgtcctgg cgtgtaacct cttaccgcaa cacaagactc
     1141 aatatattca aattcgttat gccgtggcat catttaaccc cttgtaattc atcgctataa
     1201 ttgatttaat tcacaaataa aactataac
//
LOCUS       DAHHTG010000313         1222 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.321, whole genome shotgun sequence.
ACCESSION   DAHHTG010000313 DAHHTG010000000
VERSION     DAHHTG010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1222)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1222)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1222
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.321"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            104..826
                     /gene="sdiA"
                     /locus_tag="NBI74_004699"
     CDS             104..826
                     /gene="sdiA"
                     /locus_tag="NBI74_004699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SdiA"
                     /protein_id="HCD1934897.1"
                     /translation="MQDTDFFSWLRTMLLRFQRMEAAEEVYHEIELQAQQLEYDYYSL
                     CVRHPVPFTRPKVAFYTNYPESWVSYYQAKNFLAIDPVLNPENFSQGHLMWNDDLFSE
                     AQPLWEAARAHGLRRGVTQYLMLPNRALGFLSFSRCSTREIPILSDELQLKMQLLVRE
                     SLMALMRLNDEIVMTPEMNFSKREKEILKWTAEGKTSAEIAMILSISENTVNFHQKNM
                     QKKINAPNKTQVACYAAATGLI"
     gene            complement(894..1118)
                     /locus_tag="NBI74_004700"
     CDS             complement(894..1118)
                     /locus_tag="NBI74_004700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2594 family protein"
                     /protein_id="HCD1934898.1"
                     /translation="MSTPDFSTAENNQELANEVSCLKAMLTLMLQAMGQADAGRVMLK
                     MEKQLALIEDETQAAVFSKTVKQIKQAYRQ"
BASE COUNT          325 a          254 c          286 g          357 t
ORIGIN      
        1 attcattgcc tgaataatat aaaaaatata taaatcttat ttatgtgata gtttgaatta
       61 tcattataaa tgatactcac tctcaggggc gttgcggttt actatgcagg atacggattt
      121 tttcagctgg cttcgcacga tgttgttgcg ttttcagagg atggaggccg cagaagaggt
      181 ctaccatgaa atagagcttc aggctcagca gctggagtac gattactatt cgttatgtgt
      241 tcgccacccg gtaccattca cgcgacctaa agtggctttc tacaccaatt accctgagtc
      301 gtgggttagt tattatcagg caaaaaattt tctcgcaatt gatccggtgc tgaaccctga
      361 aaactttagt cagggccatt taatgtggaa tgatgactta ttcagcgaag cacagccgtt
      421 atgggaagct gcgcgcgcac atggtttacg ccgcggtgtc actcagtatt taatgttgcc
      481 aaatcgggcg ttgggctttt tgtccttttc ccgttgcagc acgcgcgaaa tacccattct
      541 tagtgatgaa ctgcaattaa aaatgcagct actggtgcgc gaaagtctga tggctctgat
      601 gcgtttaaat gatgaaatag tgatgacgcc agagatgaat ttcagcaagc gcgaaaaaga
      661 aattctgaaa tggacggcgg aaggtaagac atcagcagag atagcgatga ttttgtcaat
      721 ctctgagaat acggtcaatt tccatcagaa aaacatgcag aaaaaaatca atgcaccgaa
      781 taagacgcag gttgcctgtt acgcggccgc tactggctta atttgatctc ttttctgttc
      841 tgcgtgccag atgcaaaaac cggctgaaag gcacgctctc agccggtttt atattactga
      901 cggtaggctt gtttaatttg cttaaccgtt ttggaaaata ctgcagcctg ggtttcgtct
      961 tcgatcagcg caagctgttt ttccatcttt aacatcacgc ggcccgcgtc agcttgtccc
     1021 atcgcctgca gcatcagcgt cagcatcgct ttcaggcagg agacttcatt tgccagttct
     1081 tgattattct cggcagtaga aaaatcaggc gtactcattt attttcctcg tcatgttgca
     1141 atgaaaattt gcggtgaaaa atgttaaggc ggcggagtat accataagct ttgctaaaaa
     1201 tagcagtggt tgttttttga gc
//
LOCUS       DAHHTG010000314         1205 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.322, whole genome shotgun sequence.
ACCESSION   DAHHTG010000314 DAHHTG010000000
VERSION     DAHHTG010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1205)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1205)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1205
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.322"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            complement(344..871)
                     /locus_tag="NBI74_004701"
     CDS             complement(344..871)
                     /locus_tag="NBI74_004701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001576751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1934899.1"
                     /translation="MATTITEITECFEYFFSSLYRREFVKTLRLNECSERELLPLVRC
                     YLLGWFADNVSPEVKSKLPGTVSGHGYIDFVIDDVAVEFAVRKPTAARSNVSATVNST
                     EVKKLMKHDGKALLVLFDFSDTPYSEEQIESFRNWPSLGRGNHRKSAFNVVYFFVEKR
                     RPLALGKITKNIRIT"
     gene            complement(883..1152)
                     /locus_tag="NBI74_004702"
     CDS             complement(883..1152)
                     /locus_tag="NBI74_004702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001531625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCD1934900.1"
                     /translation="MRNSTASQKKQIKGSSGNWHRADIVAALHKRGLTLALLSRSHGL
                     APRTLNNALERHYPRAERIIAGALDTTPETLWPERYALKNGPNSR"
BASE COUNT          289 a          305 c          272 g          339 t
ORIGIN      
        1 cctgccgcag aagccccgca gtatccctcg caaccacgtg tcataatgct ggccttaccg
       61 tttgtcagtt cactaattac tgcaacacac tcgctggcat tgtcattcca gacccagcga
      121 cgcttctgac tttctccggc agctatttgc tgtgctgtcc cctccatatt gcatacgccc
      181 gaaccatccc ccgttgaggc attaatgttg aattcagcgt tattctgtcc cggatgcagg
      241 gttagaaccg ctccgggttt tacgtactgc gctgctgcga ctggtagcgt tatggctgcc
      301 atgaataagg ccaacagttt tctgttcatt aattaattcc ctgttaagtg atacggatgt
      361 ttttggtgat tttccccagt gccagcggtc tgcgtttttc cacaaagaaa taaacgacgt
      421 tgaatgcaga cttgcgatga ttccctcgac caagagaggg ccagttgcgg aaggattcaa
      481 tctgttcttc tgaatagggt gtgtcagaga aatcaaacag cacaagcaag gctttgccgt
      541 catgtttcat caatttcttt acttctgtac tgttgacggt cgcagagaca ttggaacgtg
      601 ccgcagttgg tttacgtact gcaaactcaa cggccacatc gtcaatgaca aaatcaatgt
      661 aaccgtgccc ggatacagtt cctggcagtt tgctttttac ttccggtgag acgttatctg
      721 caaaccaacc cagcaaatag caacgtacca gaggcagtaa ttcccgctcg ctacattcgt
      781 tcagacgcag ggttttgacg aactccctgc gataaagaga actgaaaaaa tactcaaagc
      841 attccgttat ttcagtgatg gtggttgcca tggttgcatc ctttacctgc tattaggtcc
      901 atttttaagg gcataccttt ccggccataa tgtttccggt gtcgtatcca gagccccggc
      961 aataatgcgc tcagctctgg gataatgacg ctctaaggcg ttatttaatg tccgtggcgc
     1021 aaggccgtga ctgcgtgaca gtaacgccag cgttaaccct cgcttgtgaa gtgctgccac
     1081 aatatcggca cgatgccagt tccctgaact tcctttgatt tgctttttct gcgatgctgt
     1141 tgagttacgc acagtattaa ctccatggaa tcagtacatg gatattaaat gcgaataaaa
     1201 gatac
//
LOCUS       DAHHTG010000315         1195 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.323, whole genome shotgun sequence.
ACCESSION   DAHHTG010000315 DAHHTG010000000
VERSION     DAHHTG010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1195)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1195)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1195
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.323"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            116..>1195
                     /locus_tag="NBI74_004703"
     CDS             116..>1195
                     /locus_tag="NBI74_004703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCD1934901.1"
                     /translation="MKKAIAYMRFSSPGQMSGDSLNRQRRLITEWLKVNSDYYLDTVT
                     YEDLGLSAFNGKHAQSGAFSEFLDAIEHGYILPGTTLLVESLDRLSREKVGEAIERLK
                     LILNHGIDVITLCDNTVYNIDSLNEPYSLIKAILIAQRANEESEIKSSRVKLSWKKKR
                     QDALESGTIMTASCPRWLSLDDKRTAFIPDPDRVKTIELIFKLRMERRSLNAIAKYLN
                     DHAVKNFSGKESAWGPSVIEKLLANKALIGICVPSYRARGKGISEIAGYYPRVISDDL
                     FYAVQEIRLAPFGISNSSKNPMLINLLRTVMKCEACGNTMIVHAVSGRLHGYYVCPMR
                     RLHRCDRPSIKRDLVDYNIINELLFN"
BASE COUNT          403 a          178 c          257 g          357 t
ORIGIN      
        1 attattgttg tatattcaag ataatgtgac cttaattgtc tttttaaata aaaattaaac
       61 aaaaatcata tctcaccact aaggtttata aaagcatact ttagcaggtg tcaccatgaa
      121 aaaagccata gcatatatgc gattttcatc accaggtcag atgtctggtg attcattaaa
      181 ccgccagaga aggcttatta ctgaatggct aaaggtaaat agtgattatt accttgatac
      241 cgtaacgtat gaagatttgg ggttaagcgc attcaatgga aagcatgcac aatcaggagc
      301 tttttcggaa tttttagatg ctatagaaca tggttacata ttgccaggga ctacattgtt
      361 agttgaaagt ctggacagac tttcaagaga aaaagtcggt gaagcgattg agcgtctgaa
      421 attgattttg aatcacggta ttgatgttat aactctttgc gataatacag tctataatat
      481 agactcattg aatgagccat attcattaat aaaagccata cttatagcac aaagggcaaa
      541 tgaagaaagc gagataaagt caagtcgggt taaattatca tggaagaaaa aacggcagga
      601 tgcactggaa tcaggcacga ttatgacggc gtcttgtccg agatggctct cattggatga
      661 caaaagaaca gcttttattc cagaccccga cagggtgaaa acgattgagc taatttttaa
      721 actcaggatg gaaaggcgat cattgaatgc aatagctaag tatttaaatg atcatgctgt
      781 aaaaaatttt tcaggaaaag aaagtgcatg ggggccttct gtaattgaaa aattattagc
      841 gaataaagct ctgataggta tatgcgtacc ttcatatcgt gcaagaggga aagggataag
      901 tgaaatcgct ggctattatc ccagagtcat atcagatgat ttgttttacg ctgtacagga
      961 aattcggttg gcaccttttg gtattagcaa tagtagcaag aatcctatgc taataaatct
     1021 acttcgaaca gttatgaagt gtgaggcttg tggtaatacc atgattgttc atgcggtatc
     1081 tggacgtttg catggctatt atgtttgtcc gatgagaaga ttacatcgat gtgacaggcc
     1141 atcaataaaa agagatttgg ttgattataa tatcattaat gaattgcttt ttaat
//
LOCUS       DAHHTG010000316         1183 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.324, whole genome shotgun sequence.
ACCESSION   DAHHTG010000316 DAHHTG010000000
VERSION     DAHHTG010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1183)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1183)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1183
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.324"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            <1..>1183
                     /locus_tag="NBI74_004704"
     CDS             <1..>1183
                     /locus_tag="NBI74_004704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011478180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EntS/YbdA MFS transporter"
                     /protein_id="HCD1934902.1"
                     /translation="NGNTTVDGQGSTGTEIAGNNAVVNQDGTLDVSGGGHGIDITGDS
                     ATVDNKGGMTVTDPDSIGILIDGDKAIVNNDGDNAISNGGTGTQINGDEATVNNNGNT
                     TVDGQGSTGTEIAGNNVVVNQDVTLDVSGGGHGIDITGDSATVDNKGGMTVTDPDSIG
                     ILIDGDKAIVNNDGDNAISNGGTGTQVNGDEATVNNNGNTTVDGQGSTGTEIAGNNAV
                     VNQDGTLDVSGGGHGIDITGDSATVDNKGGMTVTDPDSIGILIDGDKAIVNNDGDNAI
                     SNGGTGTQVNGDEATVNNNGKTTVDGQGSTGTEIAGNNAVVNQDGTLDVSGGGHGIDI
                     TGDSATVDNKGGMTVTDPDSIGILIDGDKAIVNNDGDNAISNGGTGTQINGDDATANN
                     NG"
BASE COUNT          321 a          315 c          357 g          190 t
ORIGIN      
        1 aacggtaaca ccaccgttga tggtcagggc tctacaggta ccgaaatcgc aggtaataac
       61 gctgtagtga atcaggacgg cacgctggat gtcagcggcg gcggtcacgg aattgatatc
      121 accggcgaca gcgccacggt ggacaacaag ggcggtatga cggttaccga tccggattcc
      181 attggtattc tgatcgacgg tgataaagcc atcgtcaaca acgacggcga caatgccatc
      241 agcaacggcg gcaccggcac gcagattaat ggcgatgaag ccactgtaaa caataacggt
      301 aacaccaccg ttgatggtca gggctctaca ggtaccgaaa tcgcgggtaa caacgtggtg
      361 gtgaatcagg acgttacgct ggatgtcagc ggcggcggtc acggtattga tatcaccggc
      421 gacagcgcca cggtggacaa caagggcggt atgacggtta ccgatccgga ttccatcggt
      481 attctgatcg acggcgacaa agccatcgtc aacaatgacg gcgataacgc catcagcaac
      541 ggcggcaccg gcacgcaggt taacggtgat gaagccactg taaacaataa cggcaacacc
      601 accgttgatg gtcagggctc gaccggtacc gaaatcgcgg gtaataacgc tgtagtgaat
      661 caggacggca cgctggatgt cagcggcggc ggtcacggga ttgatatcac cggcgacagc
      721 gccacggtgg ataacaaagg cggtatgacg gttaccgatc cggactccat cggtattctg
      781 atcgacggcg acaaagccat cgtcaacaac gacggcgaca acgccatcag caacggcggc
      841 accggcacgc aggttaacgg tgatgaagcc accgtgaaca ataacggtaa aaccaccgtt
      901 gatggtcagg gctcgaccgg cacggaaatc gcgggtaata acgctgtagt gaatcaggac
      961 ggcacgctgg acgtcagcgg cggcggtcac gggattgata tcaccggcga cagcgccacg
     1021 gtggacaaca agggcggtat gacggttacc gatccggatt ccatcggtat tctgatcgac
     1081 ggtgataaag ccatcgtcaa caatgacggc gacaacgcca tcagcaacgg cggcaccggc
     1141 acacaaatta acggtgacga tgcgaccgcg aacaacaacg gca
//
LOCUS       DAHHTG010000317         1177 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.325, whole genome shotgun sequence.
ACCESSION   DAHHTG010000317 DAHHTG010000000
VERSION     DAHHTG010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1177)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1177)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1177
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.325"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            complement(1..552)
                     /gene="papA"
                     /locus_tag="NBI74_004705"
                     /pseudo
     CDS             complement(1..552)
                     /gene="papA"
                     /locus_tag="NBI74_004705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_151035091.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="P fimbria major subunit PapA"
     gene            complement(760..1073)
                     /locus_tag="NBI74_004706"
                     /pseudo
     CDS             complement(760..1073)
                     /locus_tag="NBI74_004706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001546401.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin biosynthesis transcription regulatory
                     family protein"
BASE COUNT          344 a          300 c          201 g          332 t
ORIGIN      
        1 cagcatcacc ggttgcgact gcagaaaaag caccctcagt gacttgggca ttattggctg
       61 acgacttctt aacaatcgca gtataatgca gcacattatc accatctttc agggtattag
      121 catcaccttc tgtgccatcg aaagaaacgt ttttacctgc agcctgaact acaatcgcag
      181 tacctgtccc cccgctggta tctaactcct cattatgacc agaaaccctt ggaccactaa
      241 aagccagctt cacagtccct ttttttgctg ccgagactcc tcccccccct taaatgaagt
      301 gatatcacaa ttaaccaatt caatatctaa attcattggt ttggatacgc ctcctgcttg
      361 aaggaagctt tttgaaagct gtccaaaatc aatagactga tcagctgatt tctgagaaat
      421 gctgcatgga gcatcaacaa cagttccgtt aaaggttact ctaccctgcc tctgtggagt
      481 agttagagca gcatttacac caaaagacac cactgccata gctaccgcac cggcaataac
      541 cgacttaatc ataaataaca acctcttttt cattactgaa cacaccagag atatctggca
      601 atttaaaatg acccgattac ctttaataac cagatcaata caaataatat tctcgtattt
      661 tcagggcagg ttatttatcg acagaatact gatataacct gtctgctttc tgacaagggc
      721 ttcttttttt atcttccaga aactccggaa tgccataatt tagtcaaatg ccgatgactc
      781 atctgtataa taaggtgcaa gccttgctgc aagagcattc agccgtataa gtctccccag
      841 tgttgtactg aaatacccat tattcatctg gtatttctcg cagacctcct tacgggagtg
      901 cccacctacc agatagtcct tcatagccag aatgaccctg tcactgtgaa tagaagaaat
      961 acctatcagt aaaaaaaatg catttcagac atagagccga gcaacagcac gctctcgcgt
     1021 atattcagca aaaacgcatt tcctgaccga ctgatgactt catgatgcgc catgtttccc
     1081 ccttctgtcg ggcccctgga tatatgcttc cggcccggaa atacccttca cgcaataagt
     1141 taaatttagt ttttttatgt tgtaaatatt gatttaa
//
LOCUS       DAHHTG010000318         1152 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.326, whole genome shotgun sequence.
ACCESSION   DAHHTG010000318 DAHHTG010000000
VERSION     DAHHTG010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1152)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1152)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1152
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.326"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            135..242
                     /locus_tag="NBI74_004707"
     CDS             135..242
                     /locus_tag="NBI74_004707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588455.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1934903.1"
                     /translation="MPTKRFDKKHWKMVVVLLAICGAMLLLRWAAMIWG"
     gene            complement(295..1053)
                     /locus_tag="NBI74_004708"
     CDS             complement(295..1053)
                     /locus_tag="NBI74_004708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiY family protein"
                     /protein_id="HCD1934904.1"
                     /translation="MKLLKTVPAIVMLAGGMFASLNAAADDSVFTVMDDPASAKKPFE
                     GNLNAGYLAQSGNTKSSSLTADTTMTWYGQTTAWSLWGNASNTSSNDERSSEKYAAGG
                     RSRFNLTDYDYLFGQASWLTDRYNGYRERDVLTAGYGRQFLNGPVHSFRFEFGPGVRY
                     DKYTDNASETQPLGYASGAYAWQLTDNAKFTQGVSVFGAEDTTLNSESALNVAINEHF
                     GLKVAYNVTWNSEPPESAPEHTDRRTTLSLGYSM"
BASE COUNT          326 a          281 c          266 g          279 t
ORIGIN      
        1 ttttttcata aaatgcagaa acagtgacca ggtgtacaac gccacccgac gactgtaata
       61 acaaaaaatc ccttgatgcc tgtccctttt gttacactcc gttatcacgc acaagagata
      121 tgcaggacac tggtatgccg actaaacgct ttgataaaaa acactggaag atggtggtgg
      181 tgctactggc aatctgtggc gctatgttgt tgctacgttg ggcagcaatg atttggggct
      241 gagcagtgac aaacgaaaac agccagaccg cataacacgg tccggcagat aattttacat
      301 gctgtaaccc aacgataagg ttgtccgacg atcggtatgt tccggtgcag actctggagg
      361 ttcagagttc catgtaacgt tgtaagccac cttcagtcca aaatgttcat taatagcaac
      421 atttaatgcg ctttcggagt tcagcgttgt gtcttccgcg ccaaagaccg aaacaccctg
      481 cgtaaattta gcgttgtcag tcaactgcca tgcataagcc ccggaggcat agcccagcgg
      541 ctgggtttca ctggcattgt cggtatattt gtcgtaacgc acaccagggc cgaattcaaa
      601 gcggaaactg tgtaccgggc cattcaaaaa ctggcgacca taacccgcgg tcaacacatc
      661 gcgctctcga tagccgttat aacggtctgt cagccagctt gcctgaccaa ataaatagtc
      721 ataatcagtt aaattaaaac ggctacgccc gcccgccgca tatttttctg aagaacgctc
      781 atcattagaa gaagtattac tggcgttccc ccacagcgac caggcagtgg tttgtccata
      841 ccaggtcata gtggtgtcag ccgtaagtga ggagcttttc gtattgcctg attgtgcaag
      901 atatcctgcg ttcagattac cttcgaaagg ttttttagcg ctggcaggat cgtccatgac
      961 agtaaaaacg gaatcatcgg cagctgcatt cagtgacgca aacatgcccc ccgccaacat
     1021 aacgatggca ggaactgtct tcaaaagctt catttatcaa gagtccgtac aacaaaaaaa
     1081 gagaccatcg cggtcccgga aactttctta aggataaaga ttagcgtccc tggaaaggta
     1141 acgaattata aa
//
LOCUS       DAHHTG010000319         1145 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.327, whole genome shotgun sequence.
ACCESSION   DAHHTG010000319 DAHHTG010000000
VERSION     DAHHTG010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1145)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1145)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1145
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.327"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            <1..1074
                     /locus_tag="NBI74_004709"
     CDS             <1..1074
                     /locus_tag="NBI74_004709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HCD1934905.1"
                     /translation="LTDCGETRGRWVIEGDLSSYFDTVHHRLLMKAVRRRISDARFMT
                     LLWKTIKAGHIDVGLFRAASEGVPQGGVISPLLSNIMLNEFDQYLHERYLSGKARKDR
                     WYWNNSIQRGRSTAVRENWQWKPAVAYCRYADDFVLIVKGTKAQAEAIREECRGVLEG
                     SLKLRLNMDKTKITHVNDGFIFLGHRIIRKRSRYGEMRVVSTIPQEKARNFAASLTAL
                     LSGNYSESKVDMAEQLNRKLKGWAMFYQFVDFKAKVFSYIDRVVFWKLAHWLARKYRT
                     GIASLMRWWCKSPKPGQSKTWVLFGKTNHGKLSGEILYRLVGQGKKLFRWRLPEGNPY
                     LRTETRNTYTSRFTEVAMAFASI"
BASE COUNT          301 a          269 c          335 g          240 t
ORIGIN      
        1 ctcacagact gtggtgaaac ccggggacgc tgggtgattg aaggcgacct gtccagttac
       61 ttcgacaccg tacatcatcg actgctgatg aaggctgtac gccgcaggat cagtgacgca
      121 cgtttcatga ctctgctgtg gaaaaccatc aaggcgggac atatcgatgt cggtctcttt
      181 cgggcggcca gtgaaggtgt gccacagggc ggtgttatat cgccgctatt atcgaacatc
      241 atgctgaatg agttcgatca atacctgcat gagcgctacc tgagcgggaa agccagaaaa
      301 gatcggtggt actggaataa cagtatccaa cggggccgaa gtacggcggt cagagaaaac
      361 tggcagtgga aacccgcggt ggcgtactgc cgctatgccg atgattttgt cctcatcgtc
      421 aaaggcacca aagcacaggc ggaagccatc agggaggagt gtcggggtgt gctcgaaggc
      481 agtctgaaac tcaggctgaa catggataag actaaaatca cccatgttaa tgacggcttt
      541 atctttctgg ggcacaggat cattcgcaaa cgcagtcgtt atggcgagat gcgagtggtc
      601 tcaacgatcc cgcaggagaa agccagaaac ttcgccgcat cgctgacagc actgttatca
      661 ggcaactaca gtgaaagcaa agtcgatatg gctgaacaac tcaaccgaaa actgaaaggc
      721 tgggccatgt tctatcagtt cgttgatttt aaggccaaag tcttcagtta tatcgaccgt
      781 gtcgtgttct ggaagctggc tcactggctg gcccgcaaat accgtacagg tatcgcttcc
      841 ctgatgaggt ggtggtgtaa atcaccgaaa ccgggtcaga gcaaaacgtg ggttttattt
      901 ggtaaaacca atcacggcaa gctcagcggc gaaatactgt accggttggt ggggcaaggc
      961 aagaagctgt tccgctggcg gctacccgaa ggtaatccct atctgaggac ggagaccaga
     1021 aacacgtata catcgcgctt tacagaagtg gcaatggcgt tcgccagcat ttaaatggag
     1081 agccggatgc gctgaaaggt gcacgtccgg ttcggggagg agaggcaggg aaatagtccg
     1141 actac
//
LOCUS       DAHHTG010000320         1114 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.328, whole genome shotgun sequence.
ACCESSION   DAHHTG010000320 DAHHTG010000000
VERSION     DAHHTG010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1114)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1114)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1114
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.328"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            complement(<1..110)
                     /locus_tag="NBI74_004710"
     CDS             complement(<1..110)
                     /locus_tag="NBI74_004710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416537.1"
                     /note="WbbJ; catalyzes the transfer of the O-acetyl moiety
                     to the O antigen; part of the lipopolysaccharide
                     biosynthetic pathway; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HCD1934906.1"
                     /translation="MILKLAKRYGLCGFIRLVRDVLLTRVFYRNCRIIRFP"
     gene            complement(91..1083)
                     /gene="wbbI"
                     /locus_tag="NBI74_004711"
     CDS             complement(91..1083)
                     /gene="wbbI"
                     /locus_tag="NBI74_004711"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-1,6-galactofuranosyltransferase"
                     /protein_id="HCD1934907.1"
                     /translation="MYFLNDLNFSRRDAGFKARKDALDIASDYENISVVNIPLWGGVV
                     QRIISSVKLSTFLCGLENKDVLIFNFPMAKPFWHILSFFHRLLKFRIVPLIHDIDELR
                     GGGGSDSVRLATCDMVISHNPQMTKYLSKYMSQDKIKDIKIFDYLVSSDVEHRDVTDK
                     QRGVIYAGNLSRHKCSFIYTEGCDFTLFGVNYENKDNPKYLGSFDAQSPEKINLPGMQ
                     FGLIWDGDSVETCSGAFGDYLKFNNPHKTSLYLSMELPVFIWDKAALADFIVDNRIGY
                     AVGSIKEMQEIVDSMTIETYKQISENTKIISQKIRTGSYFRDVLEEVIDDLKTR"
BASE COUNT          365 a          222 c          178 g          349 t
ORIGIN      
        1 ggaaatcgaa taattctaca gttccggtaa aatacacgag tcaataagac atctctaaca
       61 agccgaataa aaccacagag accatatcgt ttagcgagtt ttaagatcat cgatcacctc
      121 ttcaagaaca tccctgaagt aacttcctgt tcgaattttc tgagaaataa tttttgtatt
      181 ctcactaatt tgcttataag tttctattgt catggagtca acaatctctt gcatttcttt
      241 gattgatccc actgcatatc ctattctatt atctacaatg aaatccgcaa gggcggcttt
      301 atcccatata aatactggaa gttccattga aagataaaga gatgtcttat gagggttatt
      361 aaactttaaa tagtcgccaa aggcaccact acaggtttcg acagaatctc catcccaaat
      421 gagtccaaat tgcatgcctg ggaggttaat cttttccgga gattgagcat caaaacttcc
      481 aagatattta ggattatctt tattttcata gttgacacca aagagagtaa aatcgcatcc
      541 ttcagtatat atgaaagaac atttatgcct agaaaggttg ccagcatata tgacccctcg
      601 ttgcttatcc gtaacatctc gatgctccac atcagatgag acgaggtaat caaatatttt
      661 tatgtctttg attttatcct gagacatata tttactaagg tactttgtca tttgtggatt
      721 gtgacttatg accatatcac aggtagcaag ccgcacagaa tcactacccc ctcctcctct
      781 taattcatca atatcatgaa tcagaggtac tattctaaat tttagaaggc ggtgaaagaa
      841 tgacaatata tgccaaaatg gtttggccat cgggaaattg aaaattaaaa catctttatt
      901 ttcaagaccg cagagaaatg tactaagctt aacagaacta ataattctct ggactactcc
      961 accccataga ggaatgttaa caacagaaat gttttcataa tctgaagcaa tgtccagtgc
     1021 atcttttctt gctttaaatc cagcatcgcg tctagagaaa tttaaatcat tcaaaaaata
     1081 cattttcact ttattttctg ggccttaaga aatt
//
LOCUS       DAHHTG010000321         1085 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.329, whole genome shotgun sequence.
ACCESSION   DAHHTG010000321 DAHHTG010000000
VERSION     DAHHTG010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1085)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1085)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1085
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.329"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            complement(124..1038)
                     /locus_tag="NBI74_004712"
     CDS             complement(124..1038)
                     /locus_tag="NBI74_004712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCD1934908.1"
                     /translation="MGKTFSIKVLFGIYLLLMAGKVFAFSCNVDGGSSIGAGTTSVYV
                     NLDPVIQPGQNLVVDLSQHISCWNDYGGWYDTDHINLVQGSAFAGSLQSYKGSLYWNN
                     VTYPFPLTTNTNVLDIGDKTPMPLPLKLYITPVGAAGGVVIKSGEVIARIHMYKIATL
                     GSGNPRNFTWNIISNNSVVMPTGGCTVDSRNVTVNLPDFPGSAEIPLGVYCSSEQKLS
                     FYLSGATTDSARQVFANTAPDATKASGVGVSLMRNGKTLATGENVSLGTVNKSKVPLG
                     LSATYGQTGNKVAAGAVQSVIGVTFIYE"
BASE COUNT          316 a          236 c          223 g          310 t
ORIGIN      
        1 aaataagact ctggcttcaa ttgtgcgcgg attttcttac aagtgtagga taatagcctt
       61 cacaaaataa ttatccgcgc aattttcgtg aatggagcgc atagactaat gtcaaaatca
      121 caattattca tagataaaag tgacaccaat gactgactgg acggctccgg ctgcaacctt
      181 atttcctgtt tgtccatagg ttgcgcttaa acccagaggt actttgcttt tatttactgt
      241 ccccagggag acattttctc ctgttgccag tgtttttcca ttacgcatca gagagacacc
      301 cacgcccgat gcttttgttg catctggtgc tgtgttagca aacacttgtc gggcactgtc
      361 agtggtggca cccgaaagat aaaatgagag cttttgttcg ctagagcaat atacaccaag
      421 gggtatttct gcgcttccag gaaagtccgg gagattcacg gtgacattac gactatcaac
      481 ggtgcaaccg cctgttggca taacaacact gttatttgaa ataatattcc acgtaaaatt
      541 acgtggattt ccgcttccta acgtggcaat tttatacata tgaatacggg caataacttc
      601 ccctgatttt atgacaactc caccggctgc tccgactggg gtaatataga gttttaacgg
      661 taatggcatc ggtgtcttat caccaatatc caatacgttg gtatttgtcg tcaacgggaa
      721 ggggtaggtc acattattcc agtacagtga tcctttatat gactgtaggg agcctgcgaa
      781 agctgatcct tgtaccaggt ttatatgatc agtgtcatac cagccgccgt aatcattcca
      841 gcaacttata tgctgagaca agtctacaac cagattctgg cccggttgta ttacaggatc
      901 gagattaaca taaacagagg ttgtacctgc tccaatactt gacccgccat cgacattaca
      961 actgaaggca aaaactttgc ctgccattaa tagaagataa ataccgaaca ggacctttat
     1021 actgaatgtt ttacccatga tatatcctaa gtttaaaaaa ttgatttaaa aaggaggcaa
     1081 aaaaa
//
LOCUS       DAHHTG010000322         1084 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.330, whole genome shotgun sequence.
ACCESSION   DAHHTG010000322 DAHHTG010000000
VERSION     DAHHTG010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1084)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1084)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1084
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.330"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            complement(<1..529)
                     /locus_tag="NBI74_004713"
     CDS             complement(<1..529)
                     /locus_tag="NBI74_004713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001589401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HCD1934909.1"
                     /translation="MCALDRRERPLNSQSVNKYILNVQNIYRNSPVPVCVRNKNRKIL
                     YANGAFIELFSREDKPLSGESYIRLQVEIFLSSLELECQALGHGSAFCRRFNFHGEIY
                     QIRMENVSFYNDESVVLWQINPFPDYPFFALNQSGSNTNTSDKLTIWNDLSPGTLVVF
                     SFYMLGVGHATIAREL"
     gene            complement(723..1073)
                     /locus_tag="NBI74_004714"
     CDS             complement(723..1073)
                     /locus_tag="NBI74_004714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001491165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxosome protein TraM"
                     /protein_id="HCD1934910.1"
                     /translation="MVYKINKIVERRRAEGAKSTDVSFSSISTMLLELGLRVYEAQME
                     RKESAFNQAEFNKVLLECAVKTQSTVAKILGIESLSPHVSGNPKFEYANMVEDIRDKV
                     SSEMERFFPENDEE"
BASE COUNT          339 a          234 c          178 g          333 t
ORIGIN      
        1 ccaactctct ggcaattgtt gcgtgaccaa cacccagcat ataaaaagag aaaacaacca
       61 atgtccctgg agaaagatca ttccatatcg ttaatttatc agaagtattt gtattacttc
      121 cactctgatt taacgcaaaa aatggataat caggaaacgg attaatttgc cataaaacaa
      181 cagattcgtc attataaaaa gaaacattct ccatccttat ctgatagatt tcgccatgaa
      241 aattaaaacg acgacaaaat gcagagccat gtccaagagc ctggcattcc agttcaagtg
      301 atgaaagaaa aatttcaacc tgcagacgta tataactctc tccggataag ggtttatctt
      361 ctctggaaaa gagttcaata aaagccccat tggcataaag gattttccgg tttttgttac
      421 ggacacaaac cggaacggga gaatttctgt agatattctg aacgttaagg atgtatttat
      481 ttacagattg actgttaagt ggcctttctc tacgatccag cgcacacata ggaacctccg
      541 ttaaggattc tatggacagt cgatgcaggg agttcagacc tccctgcatc ggcgattttc
      601 agtttcaatt tttaacgtgg cattaaccac gtaattaata taacctttat gcggttaatt
      661 gtcatcaaat tgaaccagat caaaatcctg ttatttgaag caaatcaact tttaattctt
      721 ttttattcct catcattttc tggaaagaac ctctccatct cagatgacac cttatccctg
      781 atatcttcga ccatattcgc atattcaaac tttggattcc cggaaacatg agggcttagt
      841 gactcaatac ctaaaatctt agcaactgtt gactgtgttt ttaccgcaca ttcaagaagt
      901 accttattaa actcagcctg attgaacgct gactctttac gctccatctg agcctcatat
      961 acacgaagcc ctaattcgag aagcatagtg gatatgcttg agaaacttac atctgtactt
     1021 ttagctcctt ctgcacgtct tctttccaca attttattta ttttatagac aatttcgtca
     1081 ctga
//
LOCUS       DAHHTG010000323         1079 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.331, whole genome shotgun sequence.
ACCESSION   DAHHTG010000323 DAHHTG010000000
VERSION     DAHHTG010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1079)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1079)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1079
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.331"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            15..860
                     /locus_tag="NBI74_004715"
     CDS             15..860
                     /locus_tag="NBI74_004715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000400648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HCD1934911.1"
                     /translation="MEHLLKSESTALRESICAVIAGKDGYQADTLSLLSGVKIISRFG
                     TGIDNIDLRAAQQSGIVVNNAVGINSNAVAEFIIGLIFASMRNIPGSYHAMQNGYWGE
                     SHGCELQGKRIGLVGYGNIGKILAKRLSGFDVELLAFDKQPDYQVADKAGVQFVSIED
                     IFMQSHVIIVLLPFSSELENFISHKYLSMMRNGALIINAARGKLLDEGALLQVIEERN
                     VFAALDVFSSEPLAQFSPLLHAKNIITTPHIAAATVESYQQTGIHVAQSIIDYFAGRE
                     IKNVL"
BASE COUNT          302 a          206 c          260 g          311 t
ORIGIN      
        1 ttattaagaa taatgtggag catctgctta aatcagaatc cactgcatta cgagaatcta
       61 tttgcgcggt catcgccgga aaagatggtt atcaggcgga cacgctatca ttgctgtctg
      121 gtgtgaaaat aatatcgaga ttcgggacgg ggatagacaa tattgatctt cgtgcggcac
      181 agcaatccgg tattgttgtc aataatgcgg ttgggattaa ttcaaatgcg gttgcggaat
      241 ttatcattgg acttatcttc gcgagcatga gaaatatccc tggcagctat catgcgatgc
      301 aaaatggcta ctggggtgag tcgcatggct gtgaattaca agggaaacgg attggcctgg
      361 ttggctacgg taatataggt aaaattctgg cgaaaaggtt atccggtttt gatgttgagc
      421 ttttagcttt tgacaaacaa cccgactatc aggttgctga caaagctgga gttcagtttg
      481 tatcaattga agatatcttt atgcagtcgc atgtcatcat tgttcttttg cctttttctt
      541 ctgagctgga gaacttcatt agtcataaat atttgtcgat gatgcgcaat ggtgctctga
      601 tcatcaatgc cgcaagggga aagttgctgg atgagggcgc tttacttcag gtgattgaag
      661 aacgaaatgt gtttgcggcg ctggatgtgt ttagctctga accgttggcg caatttagcc
      721 cactgctaca tgccaaaaat atcattacaa cgccgcatat tgcggccgcc accgttgagt
      781 cataccagca aaccgggata cacgttgccc agtcgataat tgattacttc gcaggaaggg
      841 agataaaaaa tgtgttgtga gttcagcgaa caaagatttt aaatacaaaa aaaccgcaat
      901 ttgtataaca cattgcggtt tgtcagcatt ctaataaatt ggttgcgggg gccacagtca
      961 agaggttaat ccaatctttt gaaacttaag gtattcactg ttcccgaacc caagctatct
     1021 accgaaatat acactcatta tctgatgtga atcaacgagt agatcgaatg gcctgctcc
//
LOCUS       DAHHTG010000324         1053 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.332, whole genome shotgun sequence.
ACCESSION   DAHHTG010000324 DAHHTG010000000
VERSION     DAHHTG010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1053)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1053)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1053
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.332"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            195..>1053
                     /gene="rluB"
                     /locus_tag="NBI74_004716"
     CDS             195..>1053
                     /gene="rluB"
                     /locus_tag="NBI74_004716"
                     /EC_number="5.4.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2605) synthase RluB"
                     /protein_id="HCD1934912.1"
                     /translation="MSEKLQKVLARAGHGSRREIESIIEAGRVSVDGKIAKLGDRVEI
                     TPGLKIRIDGHLISVRESAEQICRVLAYYKPEGELCTRNDPEGRPTVFDRLPKLRGAR
                     WIAVGRLDVNTCGLLLFTTDGELANRLMHPSREVEREYAVRVFGQVDDAKLRDLSRGV
                     QLEDGPAAFKTIKFSGGEGINQWYNVTLTEGRNREVRRLWEAVGVQVSRLIRVRYGDI
                     PLPKGLPRGGWTELDLAQTNYLRELVELPPETSSKVAVEKDRRRMKANQIRRAVKRHS
                     QVSGGRRSGG"
BASE COUNT          259 a          246 c          304 g          244 t
ORIGIN      
        1 aaacaagctt taaccataaa gatagtgtag tatgttgcgc ctcaaagcca ggccagttaa
       61 aacgtcgagt cgtttactta aggcctgaag agttcaaaca gactttacta tcagtgagtc
      121 agatgtaagt tctgactcat tgttgcacaa aagcatatca gacgcctggg aaggcgacac
      181 caaaaggaag ctctatgagc gaaaagctac agaaagtgct ggcgcgtgcc ggccacggtt
      241 ctcgccgtga aatcgaatct attattgaag ccggtcgtgt gagtgttgat ggcaaaattg
      301 ctaaactcgg cgatcgcgtt gaaattaccc ctggactgaa aatccgtatc gatggtcacc
      361 tgatttcggt acgtgagtcc gctgaacaaa tttgtcgcgt tctggcctat tacaaaccgg
      421 aaggtgagtt gtgtacccgt aacgatccgg aagggcgtcc aacggtgttt gaccgtctgc
      481 caaagctgcg tggcgcacgc tggattgccg taggtcgtct ggacgttaac acctgtggtc
      541 tgttgttgtt caccaccgac ggtgaactgg caaaccgttt aatgcatcca agccgtgaag
      601 ttgaacgtga atatgccgtg cgtgtatttg gtcaggttga cgacgcgaaa ctgcgtgatt
      661 tgagtcgtgg cgtgcagctg gaagatggtc cggcagcctt taaaaccatc aagttcagcg
      721 gcggcgaagg gatcaaccag tggtacaacg ttactctgac cgaaggccgt aaccgtgaag
      781 ttcgtcgtct gtgggaagcg gttggtgtgc aggtgagccg cctgattcgc gttcgttacg
      841 gtgatatccc actgccaaaa ggtctgccac gcggtggctg gacggagctg gacctcgccc
      901 agactaacta tctgcgcgaa ctggtggaac taccgccaga aaccagctct aaagtcgctg
      961 tagaaaaaga ccgtcgtcgt atgaaggcga atcagattcg tcgtgcggtg aaacgtcaca
     1021 gtcaggtgag cggcggtcgt cgttctggcg gac
//
LOCUS       DAHHTG010000325         1036 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.333, whole genome shotgun sequence.
ACCESSION   DAHHTG010000325 DAHHTG010000000
VERSION     DAHHTG010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1036)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1036)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1036
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.333"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            complement(111..407)
                     /locus_tag="NBI74_004717"
     CDS             complement(111..407)
                     /locus_tag="NBI74_004717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012907439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCD1934913.1"
                     /translation="MNTVYPLKTLNQLRPLLIGFRKVNGLTQKDIAERLGVTQQTYAR
                     LEANPGSASIERLFKVFTVLGVEIELSSSLASSAINSYKLTDKYRDSLARREKW"
BASE COUNT          370 a          207 c          171 g          288 t
ORIGIN      
        1 catagtaaga acgtgtcgat gccataagca tattccggca acacgtttaa aaaaatgaat
       61 tttatcgaca tgttctaagg ttttcaccat agactatcac atagacatca tcaccacttt
      121 tctcgtctgg ctagagaatc tctatattta tcagttaact tatatgaatt tatggctgat
      181 gaagccaatg aagaagaaag ttctatttca acgcccaaca cagtaaacac tttgaacaac
      241 cgctcaatac tggcgctacc tgggttcgcc tccaatcgcg cataggtttg ctgtgttacg
      301 ccaagcctct ctgcaatatc cttttgggtc agtccattta ctttacgaaa gccaatcaga
      361 agaggacgta actgatttaa ggtcttcaac ggataaacag tattcataag gcattcacca
      421 ttttctagaa cgttttacag cctataagct gtttttaacc aatactccct atagactgta
      481 aaaaagacga cttaacctaa tgaactctac tcaatcaccg agctgaaacg ggaaattgtt
      541 aaattcccca attcaagttt ttcgcgcgaa taacttgaag aaaaggtgtg aattaattct
      601 aacgccagat ggttgggcta agagtgacaa ataagggagt tttttatctc tacataaaag
      661 agaattttac gtgcccttta tgtagagata aaagggaaaa taagctttga tgccagtgaa
      721 atcacatcaa tacctatcaa tccaacacca atcaataggt aaaagggtga tgccattggc
      781 atcacataaa cgcctatcaa tccgcaacta atcaataggc aaaaggtgat ttcaacggca
      841 tcacatgaat acctatcaat ccatcacaaa tcaatagata aaacgacaat atcactagct
      901 agccaagaga gcagtattat tccacatagt ttttagttag acatgcttgg taacaatgaa
      961 tgaaatatta ataaccatag tattgctttg atttaaaata aaggctataa aagcaaataa
     1021 ctataaaaca tgaaaa
//
LOCUS       DAHHTG010000326         1032 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.334, whole genome shotgun sequence.
ACCESSION   DAHHTG010000326 DAHHTG010000000
VERSION     DAHHTG010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1032)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1032)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1032
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.334"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            <1..>1032
                     /locus_tag="NBI74_004718"
     rRNA            <1..>1032
                     /locus_tag="NBI74_004718"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          291 a          225 c          324 g          192 t
ORIGIN      
        1 cataggttaa tgaggcgaac cgggggaact gaaacatcta agtaccccga ggaaaagaaa
       61 tcaaccgaga ttcccccagt agcggcgagc gaacggggag gagcccagag cctgaatcag
      121 tgtgtgtgtt agtggaagcg tctggaaagg cgcgcgatac agggtgacag ccccgtacac
      181 aaaaatgcac atactgtgag ctcgatgagt agggcgggac acgtggtatc ctgtctgaat
      241 atggggggac catcctccaa ggctaaatac tcctgactga ccgatagtga accagtaccg
      301 tgagggaaag gcgaaaagaa ccccggcgag gggagtgaaa aagaacctga aaccgtgtac
      361 gtacaagcag tgggagcctc ttaatggggt gactgcgtac cttttgtata atgggtcagc
      421 gacttatatt ctgtagcaag gttaaccgaa taggggagcc gaagggaaac cgagtcttaa
      481 ctgggcgtta agttgcaggg tatagacccg aaacccggtg atctagccat gggcaggttg
      541 aaggttgggt aacactaact ggaggaccga accgactaat gttgaaaaat tagcggatga
      601 cttgtggctg ggggtgaaag gccaatcaaa ccgggagata gctggttctc cccgaaagct
      661 atttaggtag cgcctcgtga attcatctcc gggggtagag cactgtttcg gcaagggggt
      721 catcccgact taccaacccg atgcaaactg cgaataccgg agaatgttat cacgggagac
      781 acacggcggg tgctaacgtc cgtcgtgaag agggaaacaa cccagaccgc cagctaaggt
      841 cccaaagtca tggttaagtg ggaaacgatg tgggaaggcc cagacagcca ggatgttggc
      901 ttagaagcag ccatcattta aagaaagcgt aatagctcac tggtcgagtc ggcctgcgcg
      961 gaagatgtaa cggggctaaa ccatgcaccg aagctgcggc agcgacacta tgtgttgttg
     1021 ggtaggggag cg
//
LOCUS       DAHHTG010000327         1022 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.335, whole genome shotgun sequence.
ACCESSION   DAHHTG010000327 DAHHTG010000000
VERSION     DAHHTG010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1022)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1022)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1022
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.335"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            15..>1022
                     /gene="waaB"
                     /locus_tag="NBI74_004719"
                     /gene_synonym="rfaB"
     CDS             15..>1022
                     /gene="waaB"
                     /locus_tag="NBI74_004719"
                     /gene_synonym="rfaB"
                     /EC_number="2.4.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418085.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide 1,6-galactosyltransferase"
                     /protein_id="HCD1934914.1"
                     /translation="MKIAFIGEAVSGFGGMETVIRDVITTFRQQHIYSEMFFFCRNDK
                     MDKGWLEGIKYSCSFSNIRLGFLRRAKHIHALSKWLQDYQPDVVICIDVISCLYAAKA
                     RKKSGIDVPVFSWPHFSLDHKKHAEYITCADYHLAISSGIKQQMINRGVPESTINVIF
                     NPVEAKDTVIPGPEEGETATFIYVGRMKFEGQKRVKDLLDGLSQAQGDWKLHVLGDGS
                     DFEKCQAYGRELKIDNRIVWHGWQKHPWEVVRQDIKKVSALLLTSSFEGFPMTLLESM
                     SWGIPCISADCVSGPGDIIQPDVNGHLYQPGDIAGFVTLLNKYIAGEIHIEHEKIPAS
                     ID"
BASE COUNT          293 a          170 c          237 g          322 t
ORIGIN      
        1 taatgagagt ttcaatgaaa atagcattta tcggcgaagc tgtttctgga tttggcggca
       61 tggaaacggt cattcgtgat gttattacta ccttcaggca acaacatatc tatagtgaaa
      121 tgtttttctt ttgtcgtaat gataaaatgg ataagggatg gctggaaggt attaagtact
      181 cgtgttcatt ttccaatatt cgcttaggtt ttttgcgtcg ggcgaaacat attcatgctt
      241 taagcaaatg gcttcaagat tatcagccag atgttgttat ttgtattgac gttatctcgt
      301 gtttgtatgc ggccaaagca cgtaaaaaat caggaattga tgtgccagta ttttcatggc
      361 cgcatttttc tttagatcac aaaaaacatg ctgaatatat cacctgtgca gactatcatt
      421 tagccatcag ctctggcatt aaacagcaaa tgattaatcg gggtgtacca gaatcgacga
      481 taaatgttat ttttaatcca gtcgaagcta aagacacagt cattcctggg cctgaggaag
      541 gtgaaacggc aacgttcatt tatgttgggc gtatgaaatt cgagggccag aagagggtta
      601 aagacctgct tgatggttta tcacaagcgc aaggtgactg gaaactccat gtactgggtg
      661 atggttctga ttttgaaaaa tgccaggcat atggtagaga attgaagatt gataatcgaa
      721 ttgtctggca tggttggcaa aaacatccat gggaggtagt gcgtcaggat attaagaaag
      781 tcagtgcatt gttattaacc tcatcttttg aaggttttcc aatgacctta cttgaatcga
      841 tgtcatgggg gattccgtgt atcagtgccg attgtgtctc cggtcctggt gacattattc
      901 aacctgatgt caatggccat ttgtatcaac cgggagatat tgctggtttt gttactcttc
      961 taaataaata tatagcaggt gaaattcata tcgaacatga aaaaattcct gcgtcgatcg
     1021 at
//
LOCUS       DAHHTG010000328         1004 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.336, whole genome shotgun sequence.
ACCESSION   DAHHTG010000328 DAHHTG010000000
VERSION     DAHHTG010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1004)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1004)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1004
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.336"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            <1..688
                     /locus_tag="NBI74_004720"
     CDS             <1..688
                     /locus_tag="NBI74_004720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001531916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1934915.1"
                     /translation="KDNFFLKKCLLDNYSDSIDTGISFLTTDHEAIVFQSSGRFVLTK
                     DVIDICNSSEEKHAEVYPPVNIGMIADMNFRKNVALIYHPMGNISGKMVYQAELSKFV
                     KKNYRSSSFLTEVDPEKIQYLITGRFFYNGSYRDLEKKSYSGPFDLVSGVISPDAKYV
                     DPFGLGCDIDKVNHSIPHVYEISTAQRVAFKSDPALSAIINKEQIDKKCRQLLKASAS
                     LKDLGGYLSQ"
BASE COUNT          336 a          146 c          197 g          325 t
ORIGIN      
        1 caaagataat tttttcttaa agaaatgtct tctggataac tacagtgaca gtatagatac
       61 aggcatttca tttttaacaa cagatcatga agcaattgtt tttcagtcat cgggtcggtt
      121 tgttttaaca aaagatgtaa tcgatatatg taattcttct gaagaaaagc atgcggaagt
      181 ttatccacct gtcaatattg ggatgattgc tgacatgaac ttcaggaaaa atgttgctct
      241 tatatatcac ccaatgggta atatttcagg aaaaatggta tatcaggctg agttgtcaaa
      301 gtttgttaaa aaaaattata gatcaagcag cttcttaacg gaggttgatc ctgaaaaaat
      361 acagtacctg ataacgggta ggttttttta caatggtagt tacagagatt tagaaaaaaa
      421 aagttattca ggcccatttg atctcgtctc aggcgttatc tctcctgatg caaaatatgt
      481 agatcctttc ggtctggggt gtgatattga taaggtcaat catagtatac cacacgtgta
      541 tgaaatatcc acagcacaac gagttgcatt taaaagcgat ccagcgttat ctgcgataat
      601 aaataaagaa caaatagata aaaagtgtcg gcagttatta aaagcatctg catctttgaa
      661 agatttaggt ggatatttaa gtcagtagaa tatgtcaccg ttactctact gtgctatagg
      721 agatagcgaa gaagagtttt ttcaatcaaa tattgccaga gttttattat tggttgaaga
      781 agacttgaag acgttggtga acaatttcaa gaggctattt tttaatgcca atggtattgc
      841 tgcaggataa gtttggcatt ttatctcagg taactttata aattatttcc ataaatgcgg
      901 aaaaatcaca aaaagctttt ttatttcttc attcttttat gataggtcgt caggaacaac
      961 actgaccagc atatttagga ggagagcgta tatatttttc acga
//
LOCUS       DAHHTG010000329          988 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.337, whole genome shotgun sequence.
ACCESSION   DAHHTG010000329 DAHHTG010000000
VERSION     DAHHTG010000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 988)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 988)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..988
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.337"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            complement(189..821)
                     /locus_tag="NBI74_004721"
     CDS             complement(189..821)
                     /locus_tag="NBI74_004721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdX family protein"
                     /protein_id="HCD1934916.1"
                     /translation="MKRLIMATMVTAILASSTVWAADNAPVAAQQQTQQVKQTQKTAA
                     AERISEQGLYAMRDVQVARLALFHGDPEKAKELTNEASALLSDDSTEWAKFAKPGKKT
                     NVNDDQYIVINASVGISESYVATPEKEAAIKIANEKMAKGDKKGAMEELRLAGVGVME
                     NQYLMPLKQTRNALADAQKLLDKKQYYEANLALKGAEDGIIVDSEALFVN"
BASE COUNT          245 a          221 c          204 g          318 t
ORIGIN      
        1 atataacata ataaatgatt tgtgataata tggaattatt gcggtttact ttgaggaaga
       61 taaaatgcga gtatatagaa ccgcgagcgc caaataattt tatgaccaca acctgtcgta
      121 ttgtggtcat aaaaaattgt cggctggagg gagttaaatc ctttacctca atatattgcc
      181 tgtcaggttt agttcacaaa cagtgcttcg ctatcaacga tgattccatc ttccgcacct
      241 ttaagcgcca ggtttgcctc gtagtattgc tttttatcaa gcagtttctg cgcatcagca
      301 agtgcattac gagtctgttt gagcggcatc agatattggt tttccattac gccaacaccg
      361 gccaggcgca gctcttccat tgcgcccttt ttatcccctt tcgccatttt ttcgttggca
      421 atttttatcg cggcttcttt ttcgggtgtc gctacataac tctcagaaat tccgacagat
      481 gcattaatga caatatattg gtcatcattg acgttggtct ttttacctgg cttagcgaac
      541 tttgcccatt cggtactatc atctgacagc aaagcggaag cttcattggt cagttctttt
      601 gccttttctg gatcgccatg aaacagagcc aggcgcgcta cctgcacgtc acgcatcgca
      661 tataaacctt gttcagagat acgctctgct gctgccgttt tctgggtttg cttaacttgc
      721 tgagtttgct gttgtgccgc taccggtgca ttatcagcag cccatacagt tgaagatgcc
      781 agaattgctg tgaccatcgt agccataatt aaacgtttca tacttacctc attctttaac
      841 agttttagaa aacgccgcca tcatattggg tgagcatgaa atgagaaatc agagagcaac
      901 ggtcattttg aaagggttat cttacaactg tagttattat cgcaaagccg cttttagtct
      961 tacagttgta ggaatctgct tcctgtcg
//
LOCUS       DAHHTG010000330          971 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.338, whole genome shotgun sequence.
ACCESSION   DAHHTG010000330 DAHHTG010000000
VERSION     DAHHTG010000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 971)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 971)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..971
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.338"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            <1..552
                     /locus_tag="NBI74_004722"
     CDS             <1..552
                     /locus_tag="NBI74_004722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCD1934917.1"
                     /translation="KTAIAAAMVMGAVVSSSAFAAGNNGTARFYGTIEDSPCSIVPDD
                     HKLEVDMGDIGAAKLTGGGTTTPKDFQIHLQDCVFTTESNMETTFTGTNYAGPANADN
                     YALFNVDSGVAMNHVSLVIGDTHGKGYKVGEKIVQPIVMDASTSKGKDKQTLNFKAWL
                     VGETDAPDLGQFETLTTFQITYL"
     gene            633..>971
                     /locus_tag="NBI74_004723"
     CDS             633..>971
                     /locus_tag="NBI74_004723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001305198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1934918.1"
                     /translation="MPNHSNFRLRGIACYIALAISGGSVNAWADDSIQFDPRFLELKG
                     DTKIDLGKFSKKGYVDAGKYNLRVFINKQSLSDEYDINWYVSENDPTKTYACLTPELV
                     AALGLKEGIAK"
BASE COUNT          268 a          229 c          235 g          239 t
ORIGIN      
        1 aaaacagcca ttgctgccgc aatggtcatg ggcgcagtcg tctccagttc tgcttttgcc
       61 gcaggtaaca acggtaccgc acgtttttac ggcactattg aagactcccc gtgctccatc
      121 gttccagacg atcataaact ggaagtcgat atgggcgata tcggtgcggc taaactgact
      181 ggcggcggca ctaccacacc gaaagatttc cagatccatt tacaggattg cgtgtttacc
      241 actgaatcaa acatggagac cacgttcacc ggcactaact acgcggggcc agccaacgca
      301 gataactatg cgttattcaa tgttgacagc ggtgttgcca tgaaccacgt gagcctggta
      361 atcggcgata cccatggcaa aggttataaa gtgggcgaaa agattgttca gccaatcgtg
      421 atggatgctt caactagcaa gggtaaagac aagcaaacgc tgaactttaa agcctggctg
      481 gtgggtgaaa ccgatgcgcc ggatctcggc cagttcgaaa ccctgaccac attccagatt
      541 acctatctct gatgtgacaa ccccacagcg ttctgctgtg gggttattca acgcacaacg
      601 gatttacgac tgtttcacaa acggatatgt gtatgcctaa tcactcaaat tttcggctgc
      661 ggggaatcgc ctgctatatt gcgctggcaa tctctggtgg atcagtcaat gcatgggctg
      721 atgattccat tcaatttgac ccccgtttcc ttgagttaaa gggcgacacg aaaattgatc
      781 tcggtaagtt ttcaaaaaaa gggtatgtcg acgcgggtaa atataattta cgtgtattta
      841 taaataaaca atccctttct gatgaatacg acattaattg gtatgtctct gaaaacgatc
      901 caacaaaaac ttatgcctgc ctgacacctg agttagtggc ggcgctgggg ctgaaagaag
      961 ggatagcaaa a
//
LOCUS       DAHHTG010000331          971 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.339, whole genome shotgun sequence.
ACCESSION   DAHHTG010000331 DAHHTG010000000
VERSION     DAHHTG010000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 971)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 971)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..971
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.339"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            <1..187
                     /locus_tag="NBI74_004724"
     CDS             <1..187
                     /locus_tag="NBI74_004724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001282578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HCD1934919.1"
                     /translation="HSAFSEWLIMAKPLEKNKMTYENNKKTHQQHIEILTAIKEHDCD
                     KAEQLMYNHLYKRIEAS"
     gene            complement(223..>971)
                     /locus_tag="NBI74_004725"
     CDS             complement(223..>971)
                     /locus_tag="NBI74_004725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001305022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCD1934920.1"
                     /translation="PQLIIFLPDKPNGTGILIIPGGGYQAVMLDKEGTALAPEFLSQG
                     YTLFVLSYRLPSPVEGENINKTFIDAISAMLYIRKNADKWHLQRHRIGVLGFSAGGHI
                     ASTFVNQICDFKRSSDSKEQEIFAIPDFVGLIYPVISMKDDVTHPGSRKQLLGDNSTS
                     ENIIQYSADLNVTSCFPPVFLLHCCDDDLVSIENSLLMYRGLIEKGVSAEMHLYEKGG
                     HGFSIQWATDLSVGGWIKLFMDWMYSHGFK"
BASE COUNT          339 a          206 c          159 g          267 t
ORIGIN      
        1 acactcagca ttttctgaat ggctgataat ggccaaacct ctggaaaaga ataaaatgac
       61 atatgaaaat aacaaaaaaa cccatcagca gcatattgaa atacttacgg ccataaaaga
      121 gcatgattgt gataaagctg aacagctaat gtataatcac ctttataaac gaattgaagc
      181 atcatagtta ctcgacgctt caccaggtag tcaccataaa tattatttaa atccatgaga
      241 atacatccag tccataaaca gttttatcca tcctccaaca gaaagatctg tggcccactg
      301 aatactaaaa ccatgccctc ctttctcata gagatgcatt tcagcagaaa cgcctttctc
      361 tattaggcca cggtacatga gcagactgtt ttcaattgac acaagatcat catcacaaca
      421 atggagtaaa aacacaggag gaaaacatga tgtgacatta agatccgcac tatattgtat
      481 tatattttct gaggtactat tgtcccccag taattgcttc ctggagcctg gatgtgtcac
      541 atcatctttc attgatatta caggataaat taacccgaca aaatcgggaa tagcgaaaat
      601 ctcttgttct ttactgtcag aagaacgttt gaaatcacaa atctgattca caaacgttga
      661 ggcgatatgc cccccagcag aaaatcctaa tacaccaata cgatgacgct gtagatgcca
      721 tttatcagca ttcttacgaa tataaagcat tgcagatatt gcatcaataa aagttttgtt
      781 tatattttct ccctcaactg gcgatggtaa tctgtaacta agaacaaata atgtatagcc
      841 ctgagataaa aactctggag caagagctgt accttcctta tctaacataa cagcctggta
      901 gccccccccg ggaataatca atattcctgt tccatttggt ttatctggta aaaaaataat
      961 taactgagga g
//
LOCUS       DAHHTG010000332          955 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.340, whole genome shotgun sequence.
ACCESSION   DAHHTG010000332 DAHHTG010000000
VERSION     DAHHTG010000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 955)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 955)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..955
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.340"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            <1..>955
                     /locus_tag="NBI74_004726"
     CDS             <1..>955
                     /locus_tag="NBI74_004726"
                     /EC_number="3.1.21.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001566505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Alw26I/Eco31I/Esp3I family type II restriction
                     endonuclease"
                     /protein_id="HCD1934921.1"
                     /translation="KDENLATNTIKKNVKPKYGDAAFLEYTRLIVNHPNYFGMPDPFG
                     EKGEIQWEAPSNRASGKFKHTHQRRYEWWKNKARSIGIDPDTEKAWISKTAKLIHPLG
                     VKPCKKCGKEMELSYSYPNEHFFSRVRKLNYIDETFELSQNEHIVDLLTRLDDRFGER
                     IYLDLPHLFSTKSITIPDISSNLEAWIEYLKEQYIPQESRMLSPGAMANPPDRFDGFH
                     SFNRCCRSIADKGRTKENLKSYVTDRRVFEYWVDGDWVAADRLMGQVRTNNIFINEEC
                     LNAGNGGLHPTPCQADHIGPISLGFSHRPQFQLLCKSCNSAK"
BASE COUNT          330 a          172 c          185 g          268 t
ORIGIN      
        1 taaagatgaa aatttagcca caaatacaat taagaaaaac gtaaaaccta aatatggcga
       61 tgcggcattt ttagagtaca ctcgactaat agttaaccac ccgaattatt ttggaatgcc
      121 agatcccttt ggggaaaaag gtgagattca atgggaagct ccatcaaata gggcatcggg
      181 gaaatttaaa cacactcatc aacggcgcta tgaatggtgg aaaaataaag ccagaagtat
      241 tgggatagac cctgacactg aaaaagcatg gattagtaaa acagctaaac taattcatcc
      301 acttggagtt aaaccatgca agaaatgcgg taaagaaatg gaattatctt actcctaccc
      361 aaatgagcat ttcttttctc gggttaggaa acttaattat atagatgaga cttttgagct
      421 atctcaaaat gaacacatag ttgatttgtt gactcgttta gatgatagat ttggtgaaag
      481 aatatattta gatttaccac atttattttc tacaaaatcc atcacgattc cagatatttc
      541 aagcaatctc gaagcatgga ttgagtatct aaaagaacaa tacatacctc aagaatcacg
      601 tatgcttagc cctggagcca tggcaaatcc tccagatcgt tttgatggat ttcattcatt
      661 taaccgctgt tgtcgctcta ttgctgataa aggaaggacc aaagaaaatc ttaaatctta
      721 tgttactgac agaagagttt tcgaatattg ggtggatggt gattgggtag ctgcagacag
      781 gctaatgggc caagttagaa caaataatat atttattaac gaggaatgtt tgaatgccgg
      841 aaatggtggt ttacatccaa ctccttgcca agcagatcac attggcccaa tctcactagg
      901 gttttcccat cgcccacaat ttcaactact ttgcaaatca tgtaatagtg ccaaa
//
LOCUS       DAHHTG010000333          950 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.341, whole genome shotgun sequence.
ACCESSION   DAHHTG010000333 DAHHTG010000000
VERSION     DAHHTG010000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 950)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 950)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..950
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.341"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            complement(<1..718)
                     /locus_tag="NBI74_004727"
     CDS             complement(<1..718)
                     /locus_tag="NBI74_004727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001606056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HCD1934922.1"
                     /translation="MIIEKVMNNNCVQASMNGQEVIISGPGVGYNKKYGMSVPEHPAN
                     RIFHVRNEQKNKLYKLIEHVDIEYVFVAEKIVQYAEKNLEKNLNPSLLLILADHISNA
                     ISRVASGIQINNVFLDEIKALYKAEYAISRDALTIINEQFSVQLPDDEIGFIALHILN
                     NYENSVDYESVRIIELSQIITGLIEVVYNRKVDRSSFNYSRFMMHLKYFSSRVLCNEK
                     IKQKDIGDIYEQFLEKDILLQ"
BASE COUNT          303 a          185 c          147 g          315 t
ORIGIN      
        1 gctgtaacag gatgtccttt tcaagaaact gttcatagat atcaccaata tctttctgtt
       61 ttattttttc attgcataat acgcgacttg aaaaatattt aaggtgcatc ataaatctgg
      121 aatagttaaa tgaacttctg tccacctttc tgttataaac aacttcaata agccccgtga
      181 ttatttgcga caactcaata atccgtactg attcataatc aactgaattt tcataattat
      241 ttaaaatatg caatgctata aaaccaatct catcatcagg aagttgaacg ctgaattgtt
      301 cattaatgat agttaatgca tcgcgactta tcgcatactc tgctttgtac aacgctttga
      361 tttcatcaag gaaaacatta ttaatttgta tacctgaagc gactcgggat attgcattcg
      421 aaatgtgatc cgcaagaatc aatagtagcg atggattgag atttttttcg agatttttct
      481 ccgcatattg cactattttt tcggcaacaa acacatactc aatatctaca tgttcaatca
      541 atttatagag tttgtttttt tgttcatttc tgacatgaaa aatccggtta gccggatgct
      601 cagggaccga cattccatat tttttgttgt aaccgacgcc aggcccagaa atgataacct
      661 cctgtccatt catcgatgcc tgtacacaat tattgttcat gactttttcg atgatcattc
      721 catggctcca aaaaaaaagg caaggcacgt cagaagtaat aactacttca aacattgcct
      781 tgcctgattt aacagttaca agatgattaa ctaatgcgta tacattacac ggaattgtgc
      841 ttaattaaag agatggtcgg cttatttttc aagccgacat tcaaatatcc gacatcagtc
      901 acaaaaccag cgtctaatat tcaggtaaat gttatctatt caacgaaata
//
LOCUS       DAHHTG010000334          935 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.342, whole genome shotgun sequence.
ACCESSION   DAHHTG010000334 DAHHTG010000000
VERSION     DAHHTG010000334.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 935)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 935)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..935
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.342"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            complement(<1..466)
                     /locus_tag="NBI74_004728"
     CDS             complement(<1..466)
                     /locus_tag="NBI74_004728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HCD1934923.1"
                     /translation="MGKSIVVVSAVNFTTGGPFTILKKFLAATNNKENVSFIALVHSA
                     KELKESYPWVKFIEFPEVKGSWLKRLHFEYVVCKKLSKELNATHWICLHDITANVVTK
                     KRYVYCHNSAPFYKGILFREILMEPSFFLFKMLYGLIYKINIKKNTAVFVQQF"
     gene            complement(451..>935)
                     /locus_tag="NBI74_004729"
     CDS             complement(451..>935)
                     /locus_tag="NBI74_004729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416537.1"
                     /note="WbbJ; catalyzes the transfer of the O-acetyl moiety
                     to the O antigen; part of the lipopolysaccharide
                     biosynthetic pathway; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HCD1934924.1"
                     /translation="PCYIRNDGSINFGENFTSGVGLRLDAFGRGVIFFSDNVQVNDYV
                     HIASIESVTIGRDTLIASKVFITDHNHGSFKHSDPMSSPNIPPDMRTLESSAVVIGQR
                     VWLGENVTVLPGTIIGNGVVVGANSVVRGSIPENTVIAGVPAKIIKKYNHETKLWEKA
                     "
BASE COUNT          306 a          195 c          155 g          279 t
ORIGIN      
        1 agaattgttg aacaaacact gcagtatttt ttttaatgtt tattttatat atcagcccgt
       61 atagcatttt aaataagaaa aagctaggct ccataagaat ttcacggaat aaaattcctt
      121 tataaaaagg ggcagagtta tgacaataca catatctttt tttagtgacg acattggccg
      181 taatatcatg cagacaaatc caatgcgtag cattcagctc ttttgaaagt tttttacaaa
      241 ctacatattc aaagtgcaaa cgttttagcc acgacccttt aacctcagga aactcaatga
      301 atttaaccca tggataactt tcttttaact ctttagcaga atggactaat gcgataaaac
      361 tgacattttc tttattatta gttgctgcca aaaatttttt caaaatggta aatggaccgc
      421 cagtggtaaa attgaccgca gaaacaacga ctatgctttt tcccataatt tggtctcatg
      481 attgtatttc tttatgattt ttgctggtac tcccgcaatg acagtatttt cgggaataga
      541 acctctaaca acagaattgg cgccgactac gactccatta ccaataattg ttccaggcaa
      601 aaccgtcaca ttctcaccca accaaaccct ctggccaatt acaacagctg aagattccaa
      661 cgtgcgcatg tctggaggta tatttggcga actcattgga tcagagtgct taaaggaacc
      721 gtgattatga tcggtaataa atactttact tgcaataagc gtatcccgac ctatcgtaac
      781 gctctcaatt gaggcgatat gaacatagtc gttaacttgc acattatcgg aaaaaaaaat
      841 cacgccacgt ccaaatgcat ccagcctgag accgactcca cttgtgaaat tttcaccaaa
      901 attaatgcta ccatcattgc gaatatagca gggaa
//
LOCUS       DAHHTG010000335          935 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.343, whole genome shotgun sequence.
ACCESSION   DAHHTG010000335 DAHHTG010000000
VERSION     DAHHTG010000335.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 935)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 935)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..935
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.343"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            complement(<1..825)
                     /locus_tag="NBI74_004730"
     CDS             complement(<1..825)
                     /locus_tag="NBI74_004730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HCD1934925.1"
                     /translation="MNDRLCFEVHDNQGYFVFPDTWFGPLLGEFEEVLDAYDADEISE
                     TSYINKLRRLAQREPDFIDIHAHLAYAFLEQNAPRKALNAALKGLAAGNRIIPESFCG
                     EIIWMHPENRPYLRALYAAILANVHLQRHQDAVMLTDKILAYNPEDNQGARWLLGSEL
                     LRTGDHERAFSVLKEHADEFSPYWYELGLLHFLNGEHVKAATAFRHGFATNTYIAEML
                     CGNLHPFPLAVWHDFSGSLDTAEDYYATYSPLWGQYPEALLFVNWLYNHSSVLHERA"
BASE COUNT          250 a          221 c          220 g          244 t
ORIGIN      
        1 tgcccgttca tgcagcacag aggaatgatt atagagccag tttacaaaca gcaatgcttc
       61 cggatattgt ccccataatg gtgagtatgt tgcatagtaa tcttctgccg tatccagaga
      121 gccggagaag tcatgccaca cagcaagtgg aaatggatgc agatttccgc acagcatttc
      181 tgcgatgtaa gtgtttgtgg caaagccatg gcgaaaagct gtggcggcct taacatgttc
      241 cccgttcaga aaatgcagga gaccgagttc ataccagtaa ggtgaaaatt catcagcatg
      301 ctctttcagt acactgaagg ctcgctcatg atcgccggtg cgtaacagct ctgaacccaa
      361 caaccagcga gccccctgat tatcttcagg attgtatgcc agtattttat cagtgagcat
      421 aaccgcatcc tgatggcgct gtaagtgcac atttgcgaga attgctgcat aaagcgcgcg
      481 cagataaggc cggttttcgg gatgcatcca gattatttca ccacagaaac tttccgggat
      541 gatacgatta cctgccgcca gtcctttaag agcggcgttc agtgccttac gcggggcatt
      601 ctgttccaga aatgcatatg ccagatgagc gtgtatatca ataaagtccg gctcccgttg
      661 agcaaggcgt cggagtttgt tgatgtaact cgtctcgctg atttcatcag cgtcgtatgc
      721 atcaagcact tcttcaaact cccccagcaa tgggccaaac caggtatccg gaaatacgaa
      781 ataaccctgg ttatcgtgca cttcaaagca taatctatcg ttcattttct gagcctgtaa
      841 aacatgtggt gatatagtct gagagcataa aagttgagat tctgtgtatc aagtcctgtc
      901 ttggcactga atgaatattg cttacaaagc ccctt
//
LOCUS       DAHHTG010000336          933 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.344, whole genome shotgun sequence.
ACCESSION   DAHHTG010000336 DAHHTG010000000
VERSION     DAHHTG010000336.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 933)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 933)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..933
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.344"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            complement(<1..773)
                     /locus_tag="NBI74_004731"
     CDS             complement(<1..773)
                     /locus_tag="NBI74_004731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001335741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter domain-containing protein"
                     /protein_id="HCD1934926.1"
                     /translation="MQRKTLLSACIALALSGQGWAADITEIETTTGEKKNTNVTCPAD
                     PGKLSPEELKRLPSECSPVVEQNLMPWLATGAATALITALAIVELNDDDDHHHRNNSP
                     LPPTPPDDNSDDTPVPPTPGGDEIIPDDGSDDTPTPPKPISFNNDVILDKTAKTLTIR
                     YSVFTYTENADGTISLQDSNGRKATINIWQIDEANNTVALDGVSADGATKWQYNHNGE
                     LVITGDNATVNNNGKTIVDGKDSTGTEIAGNNGKVIQDG"
BASE COUNT          243 a          200 c          205 g          285 t
ORIGIN      
        1 ataccgtcct gaataacttt cccgttatta ccggcgatct ccgtgccagt ggaatcttta
       61 ccatcaacga tggttttgcc attgttgttt actgtggcat tatcacccgt aataacaagc
      121 tcaccgttat gattatattg ccacttcgtt gcgccatctg cgctcacccc gtcaagggca
      181 accgtgttat tcgcttcatc aatttgccaa atattaatag ttgccttacg gccattgcta
      241 tcttgtagag aaatagtccc gtcagcattc tcggtataag taaaaactga atagcgaata
      301 gttaacgttt ttgctgtttt atcgaggata acgtcattat taaacgaaat tggtttggga
      361 ggcgtcgggg tatcatccga accgtcgtct ggtattatct catctccgcc aggagttggg
      421 ggaactggag tgtcgtctga attatcatca ggtggagttg gtggaagtgg agaattattg
      481 cgatgatgat gatcatcatc gtcgtttagt tccactatgg ctaaggctgt gattaacgca
      541 gtagcagcac ctgtggcaag ccatggcatc aggttctgtt cgactacagg ggagcattca
      601 gagggtaagc gtttaagctc ttctggactg agttttccag ggtctgccgg acaagtcaca
      661 ttggtatttt tcttttcacc tgtggtggtt tctatctctg tgatatctgc cgcccagccc
      721 tgaccactca gagccaatgc aatacaggct gacaatagag ttttcctttg cataataaat
      781 ccttctggcg aatataaaga aggtcggatg ccttaaccca accaattaat caatcaatta
      841 aaacatagag gacattcaat attgccagta gattggcacc taatcaaaca tattctctga
      901 caatctgtaa ttataatttg agtgaatgaa tac
//
LOCUS       DAHHTG010000337          916 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.345, whole genome shotgun sequence.
ACCESSION   DAHHTG010000337 DAHHTG010000000
VERSION     DAHHTG010000337.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 916)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 916)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..916
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.345"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            231..833
                     /gene="fimB"
                     /locus_tag="NBI74_004732"
     CDS             231..833
                     /gene="fimB"
                     /locus_tag="NBI74_004732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria switch DNA invertase FimB"
                     /protein_id="HCD1934927.1"
                     /translation="MKNKADNKKRNFLTHSEIESLLKAANTGPHAARNYCLTLLCFIH
                     GFRASEICRLRISDIDLKAKCIYIHRLKKGFSTTHPLLNKEVQALKNWLSIRTSYPHA
                     ESEWVFLSRKGNPLSRQQFYHIISTSGGNAGLSLEIHPHMLRHSCGFALANMGIDTRL
                     IQDYLGHRNIRHTVWYTASNAGRFYGIWDRARGRQRHAVL"
BASE COUNT          275 a          170 c          197 g          274 t
ORIGIN      
        1 ccgattgata aatatataaa ggtacatagc atgcaagagc atggcgtttg tatggcaaag
       61 ttattataag taactatcgc tactccattt aagttcactc agaagaactg gtccacttac
      121 gttagttatt aagcaaacgt tcgcttttat aaacataatc aggataaaaa tgttggatta
      181 ttgctaaccc agcacagcta gtgcgcgtct gtaattctaa gggaaaaacg atgaagaata
      241 aggctgataa caaaaaaagg aacttcctga cccatagtga aatcgaatca ctccttaaag
      301 cagcaaatac cgggcctcat gcagcacgta attattgtct gactttgctt tgttttattc
      361 atggtttccg ggcgagtgaa atttgtcgat tgaggatttc ggatattgat cttaaggcaa
      421 agtgtatata tatccatcga ttaaaaaaag gcttttcaac aacgcatccg ctattgaaca
      481 aagaagttca ggctttaaaa aactggttga gtatccgtac ttcgtacccg catgctgaga
      541 gcgagtgggt atttttatct cgtaagggaa atccgctttc tcggcaacag ttttaccata
      601 ttatctcgac ttccggtggt aatgccgggt tgtcactgga gattcatccg cacatgttac
      661 gccattcgtg tggttttgct ttggcgaata tgggaataga tacgcgactt atccaggatt
      721 atcttgggca tcgcaatatt cgtcatactg tctggtatac cgccagcaat gcagggcgtt
      781 tttacggcat ctgggataga gccagaggac gacagcgtca cgctgtttta tagagatagt
      841 tgagatacca gggatggtgt ttaaaactac attattctta cgatatatca aaaatgatta
      901 accatttatt aattga
//
LOCUS       DAHHTG010000338          910 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.346, whole genome shotgun sequence.
ACCESSION   DAHHTG010000338 DAHHTG010000000
VERSION     DAHHTG010000338.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 910)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 910)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..910
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.346"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            <1..715
                     /gene="ydeO"
                     /locus_tag="NBI74_004733"
     CDS             <1..715
                     /gene="ydeO"
                     /locus_tag="NBI74_004733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000060456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acid stress response transcriptional regulator
                     YdeO"
                     /protein_id="HCD1934928.1"
                     /translation="NILDKDYAFSDGEILMVDNAVRTHFEPYERHFKEIGFNENTIKK
                     YLQCTNIQTVTMPVPAKFLRASNVPTGLLNEMIAYLNSEERNHHNFSELLLFSCLSIF
                     AACKGFITLLTNGVLSVSGKVRNIVNMKLAHPWKLKDICDCLYISESLLKKKLKQEQT
                     TFSQILLDARMQHAKNLIRVEGSVNKIAEQCGYASTSYFIYAFRKHFGNSPKRVSKEY
                     RCQRHTGMNTDNTMNALAS"
BASE COUNT          281 a          182 c          180 g          267 t
ORIGIN      
        1 taacatttta gataaagatt acgccttctc tgacggcgag atcctgatgg tagataacgc
       61 tgttcgcacg cattttgaac cttatgagcg gcatttcaaa gaaatcggat ttaatgaaaa
      121 taccattaaa aaatatctac aatgcactaa catccagaca gtgacgatgc ctgttcctgc
      181 gaagttttta cgtgcttcaa atgtaccgac tggattgctt aatgaaatga ttgcttatct
      241 caactcggaa gaacgcaatc atcataattt ttcagagctt ttgctttttt cttgcctgtc
      301 tatttttgcc gcatgcaaag gtttcattac actattaact aacggtgtgc tatccgtttc
      361 cgggaaagta agaaatattg tcaacatgaa gctggcgcac ccatggaagc tgaaagatat
      421 ttgtgactgc ctgtacatca gtgaaagcct gttgaagaaa aaactaaagc aagagcaaac
      481 gacattctca cagattcttt tagatgccag aatgcagcac gcgaaaaatt tgatacgcgt
      541 agaaggttca gtcaataaaa ttgctgaaca atgcggttat gccagtacat cttattttat
      601 ttatgcgttt cgcaaacatt tcggcaacag tccgaagaga gtttctaagg agtaccgttg
      661 tcaaaggcac acgggtatga acacggacaa cacgatgaat gctttagcta gttgattatt
      721 tgctaacgag tagtcaacca cacacgctgc gtaagaatta aatggggcag ccattccctg
      781 ccccgcgttg tttccaggcg atatatttct tgaaataaat aagtgacagc catcacatat
      841 ttatgcattt tcataacctg ttgcatgatt atttatgatc tcagctcggc aatttgtcag
      901 tgaaatacaa
//
LOCUS       DAHHTG010000339          905 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.347, whole genome shotgun sequence.
ACCESSION   DAHHTG010000339 DAHHTG010000000
VERSION     DAHHTG010000339.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 905)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 905)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..905
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.347"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            <1..825
                     /locus_tag="NBI74_004734"
     CDS             <1..825
                     /locus_tag="NBI74_004734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001565430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2686 family protein"
                     /protein_id="HCD1934929.1"
                     /translation="NALQSQIITDNHFLHHPKVDSELTRKHERGRMDTENIYLPPLAR
                     GNNHNYDGKSVVEIRKLDISKESWPFNYVTETCREFNGITTTGRMLYRNLKITSALDE
                     IYGGICKKAHAATELAEGLRLNLFMKSPFDPVEDYTVHEITLGPGCNVPGYAGTTIGY
                     ISTLPTSQAKRWTNEQPRIDIYIDQIMTVSGVANSSGFALAALLNANIELGNDPIIGI
                     EAYPGTAEIHAKMGYKVIPGDENAPLKRMTLQPSSLPELFELKNGEWNYMVMLPTY"
BASE COUNT          280 a          183 c          190 g          252 t
ORIGIN      
        1 aatgcccttc agagtcaaat tattacagat aatcacttcc ttcatcatcc aaaagtcgac
       61 agtgagttaa cacgtaaaca tgaacgtgga cgcatggata cggaaaatat ctatcttccg
      121 cctcttgctc gtggcaataa ccataattac gatggaaaaa gcgttgtcga aattagaaaa
      181 cttgatatta gtaaagagtc ctggccattt aattatgtga cagaaacatg cagagaattc
      241 aatggtatta caacaacagg acgtatgctc tatcgaaact tgaaaatcac ctcagcttta
      301 gacgaaatat atggtggtat atgtaaaaag gcgcatgctg ccacagagtt agcagaagga
      361 ttacggctaa atttatttat gaaaagccct ttcgatcctg ttgaggacta tacagttcat
      421 gaaataacat taggccctgg ctgcaatgtt ccaggttacg ccggaaccac gataggatat
      481 atttcaactc ttccaacttc tcaggcaaaa agatggacca atgagcagcc aaggattgat
      541 atctatattg atcagataat gactgtctca ggggtggcaa actcttccgg attcgccctt
      601 gccgcattac ttaatgctaa tattgagttg ggtaatgacc caataattgg tatagaagcc
      661 tatcccggta ccgcggagat ccatgcaaaa atgggatata aagttatccc gggtgatgaa
      721 aatgcaccac tcaaacgtat gactctgcaa ccgtcatctc ttccagagct gtttgaatta
      781 aaaaatggtg aatggaatta tatggtgatg ctgccaactt actgatttag cgtatgatgg
      841 tgtttttgag gtgctccagt ggcttctgtt tctatcagct gtccctcctg ttcagctact
      901 gacgg
//
LOCUS       DAHHTG010000340          874 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.348, whole genome shotgun sequence.
ACCESSION   DAHHTG010000340 DAHHTG010000000
VERSION     DAHHTG010000340.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 874)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 874)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..874
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.348"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            <1..>874
                     /gene="glf"
                     /locus_tag="NBI74_004735"
     CDS             <1..>874
                     /gene="glf"
                     /locus_tag="NBI74_004735"
                     /EC_number="5.4.99.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
                     /protein_id="HCD1934930.1"
                     /translation="VEFNRFTNSPLAIYKDKLFNLPFNMNTFHQMWGVKDPQEAQNII
                     NAQKKKYGDKVPENLEEQAISLVGEDLYQALIKGYTEKQWGRSAKELPAFIIKRIPVR
                     FTFDNNYFSDRYQGIPVGGYTKLIEKMLEGVDVKLGIDFLKDKDSLASKAHRIIYTGP
                     IDQYFDYRFGALEYRSLKFETERHEFPNFQGNAVINFTDANVPYTRIIEHKHFDYVET
                     KHTVVTKEYPLEWKVGDEPYYPVNDNKNMELFKKYRELASREDKVIFGGRLAEYKYYD
                     MHQVISAALYQVKNI"
BASE COUNT          305 a          135 c          182 g          252 t
ORIGIN      
        1 gtagaattta atcgttttac taattctcca ctggcgattt ataaagacaa attattcaac
       61 cttcctttta atatgaatac tttccaccaa atgtggggag ttaaagatcc tcaagaagct
      121 caaaatatca ttaatgctca gaaaaaaaag tatggtgaca aggtacctga aaatttggag
      181 gagcaggcga tttcattagt tggggaggac ttataccaag cattgataaa gggttatacg
      241 gagaagcagt ggggaagaag tgcaaaagaa ttgcctgcat ttattattaa gcgaatccca
      301 gtgagattta cgtttgataa caattatttt tccgatcgct atcaaggtat tccggtggga
      361 ggctacacta agcttattga aaaaatgctt gaaggtgtgg acgtaaaatt aggcattgat
      421 tttttgaaag acaaagattc tctagcgagt aaagcccata gaatcatcta cactggaccc
      481 attgatcagt acttcgacta taggtttgga gcgttagaat atcgctcttt aaaatttgag
      541 acggaacgcc atgaatttcc aaacttccaa gggaatgcag taataaattt cactgatgct
      601 aatgtaccat ataccagaat aattgagcat aaacattttg actatgttga gacaaagcat
      661 acggttgtta caaaagaata tccattagag tggaaagttg gcgacgaacc ctactatcca
      721 gttaatgata ataaaaacat ggagcttttt aagaaatata gagagttagc tagcagagaa
      781 gacaaggtta tatttggcgg gcgtttggcc gagtataaat attatgatat gcatcaagtg
      841 atatctgccg ctctttatca agtgaaaaat ataa
//
LOCUS       DAHHTG010000341          836 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.349, whole genome shotgun sequence.
ACCESSION   DAHHTG010000341 DAHHTG010000000
VERSION     DAHHTG010000341.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 836)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 836)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..836
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.349"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            complement(<1..740)
                     /locus_tag="NBI74_004736"
     CDS             complement(<1..740)
                     /locus_tag="NBI74_004736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HCD1934931.1"
                     /translation="MDQKLLTDFRSELLDSRFGAKAISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNARQNLATFCQTWDDENVHKLMDLSINKNWIDKEEYPQSAAIDLRCVN
                     MVADLWHAPAPKNGQAVGTNTIGSSEACMLGGMAMKWRWRKRMEAAGKPTDKPNLVCG
                     PVQICWHKFARYWDVELREIPMRPGQLFMDPKRMIEACDENTIGVVPTFGVTYTGNYE
                     FPQPLHDALDKFQADTGIDIDMHIDAASG"
BASE COUNT          204 a          210 c          207 g          215 t
ORIGIN      
        1 ccgctggcgg cgtcgatgtg catgtcgatg tcgataccgg tgtcggcctg gaatttatcc
       61 agcgcatcgt gcagcggctg cgggaactca tagttaccgg tgtaggtcac gccgaaagtc
      121 ggcaccacgc cgatggtgtt ttcgtcgcag gcttcaatca tgcgtttcgg gtccataaac
      181 aactgaccgg ggcgcatagg gatctcacgc aactccacat cccagtagcg ggcgaattta
      241 tgccagcaga tttgtaccgg accgcacacc aggtttggtt tatccgtggg tttgcctgcg
      301 gcttccatac gcttgcgcca acgccatttc atcgccatcc cgccgagcat acaggcctcg
      361 gaagaaccaa tggtgttggt gccaacggcc tgaccatttt tcggtgcagg cgcatgccac
      421 agatcggcaa ccatgtttac gcaacgcaga tcgattgctg cagattgcgg atattcttct
      481 ttgtcgatcc agtttttatt gatcgacaaa tccatcaatt tatggacgtt ttcgtcgtcc
      541 caggtctggc agaaagtggc caggttctga cgagcgttgc catcaagata taattcatca
      601 ttgataatct gaaatgcaac atcatcgcgc atttcgtgca gcggaaatcg ttttgactcc
      661 gcgatagtag aaatggcctt tgcaccaaaa cgtgaatcga gtagttctga gcggaaatcc
      721 gttaacagct tctggtccat ttcgaactcc ttaaatttat ttgaaggcaa taaaaaagta
      781 ggatttatcc gcaatggaag caaggcatta caggcttaat ttaaataaca aaatcc
//
LOCUS       DAHHTG010000342          819 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.350, whole genome shotgun sequence.
ACCESSION   DAHHTG010000342 DAHHTG010000000
VERSION     DAHHTG010000342.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 819)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 819)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..819
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.350"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            <1..>819
                     /locus_tag="NBI74_004737"
     CDS             <1..>819
                     /locus_tag="NBI74_004737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HCD1934932.1"
                     /translation="QLEKTVRYLTQTVHPRSADNIDNLNRSAEYIKEVFISSGARVTA
                     QDIPITGGPYKNIVANYGPADGPLIIIGAHYDSVSSYENDQLTYTPGADDNASGVAGL
                     LELARLLQQQAPKTGVQLVAYASEEPPFFRSDEMGSAVHAASLERPVKLMIALEMIGY
                     YDSTPGSQDYPSPAMSWLYPDRGDFIAVVGRMQDINAVRQVKAALLSSRDLSVYSMNA
                     PGFIPGIDFSDHLNYWQYDIPAVMITDTAFYRNKQYHFPGDIADRLNYQKMAQV"
BASE COUNT          214 a          190 c          196 g          219 t
ORIGIN      
        1 agcaattaga aaaaacagtt cgttatctta cacaaactgt gcatccacgt agcgccgaca
       61 atattgataa tctgaataga tccgcagagt atataaaaga ggtctttatc agtagcggtg
      121 ccagagttac cgcgcaggat atccccatta cgggtggccc ctacaaaaac attgttgcta
      181 attatggtcc tgccgatgga ccgctgatta ttattggtgc gcactatgac tctgtcagta
      241 gttacgaaaa cgatcaattg acctatacgc cgggcgcgga tgataacgcc agcggtgtgg
      301 caggattact cgaactggca cgtttgttac agcagcaagc accgaaaaca ggcgtgcagc
      361 tggtcgccta tgcgtcggaa gaaccgccct tctttcgtag cgatgaaatg gggagcgcgg
      421 tacatgcagc ttcgcttgag cgtccagtga aattaatgat agcgctggag atgattggtt
      481 attacgactc tacgcctggt agccaggatt acccatcccc ggccatgtcc tggctttatc
      541 ccgatcgggg agactttatt gccgtggtcg gcagaatgca ggatatcaac gccgttcgtc
      601 aggtaaaagc ggcattgttg tcatcccggg atttatctgt ttattctatg aatgccccag
      661 gttttattcc cggtattgat ttctctgatc accttaatta ttggcaatac gatattcccg
      721 ccgtaatgat tactgacacc gctttttatc gtaataaaca ataccacttt cccggtgata
      781 tcgcagacag attaaattat cagaaaatgg ctcaggtag
//
LOCUS       DAHHTG010000343          816 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.351, whole genome shotgun sequence.
ACCESSION   DAHHTG010000343 DAHHTG010000000
VERSION     DAHHTG010000343.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 816)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 816)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..816
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.351"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            <1..>816
                     /locus_tag="NBI74_004738"
     CDS             <1..>816
                     /locus_tag="NBI74_004738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001576403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HCD1934933.1"
                     /translation="GYIIAYSLPDSALSCFKTSKKLHDVCNLKQGLITGDNERYLRFW
                     HEISYNSFSLNEKRKKTKWFPYQKGGAYRKWYGNNDYVVDWENDGYSIKNFYNDKGKL
                     RSRPQNIQFYCKEGLTWTSLTISSLSMRYVPNGYIFDAKGPMCFPKSSLDIWNILGYA
                     NSKVIDIFLKQLAPTMDYSQGPVGNVPFKFNDGDLNEIIKELVNIHKRDWDENETSFE
                     FKRDMLVHFSRDINTIKGSFTLRQGENKKAINRTKFLEEMNNSFFINCFNLTDI"
BASE COUNT          297 a          122 c          142 g          255 t
ORIGIN      
        1 ggatatatca ttgcttactc tctgcctgat tctgcgttat cttgcttcaa aacatccaaa
       61 aaacttcatg atgtttgcaa tctaaaacaa ggattaatta ctggtgataa tgaaagatac
      121 ctaagattct ggcatgaaat cagctataac tctttcagtc tcaatgaaaa aagaaaaaaa
      181 acaaaatggt tcccatatca aaaaggtggt gcataccgta aatggtatgg taataatgat
      241 tatgttgttg actgggagaa tgatggttat tccattaaaa acttttataa tgacaaaggt
      301 aaattacgct cacgccctca aaacatacaa ttttattgta aagagggttt aacatggaca
      361 agtttaacca tttcgtcact atcgatgaga tatgtaccaa atggatatat ttttgatgca
      421 aaaggaccta tgtgttttcc gaaatcctct ttggatatct ggaatattct tggctatgcg
      481 aatagcaaag taatagatat atttctcaaa caattagcgc ccaccatgga ttattctcaa
      541 gggcctgttg gaaatgtccc attcaaattt aacgatggtg atttgaacga gataataaaa
      601 gaactcgtaa acattcacaa acgtgactgg gatgaaaatg aaacatcttt tgagtttaag
      661 agagatatgt tggttcattt ttcaagagat attaacacta ttaagggtag ttttacacta
      721 aggcaagggg aaaataaaaa agcgattaac agaacaaaat ttttagaaga aatgaataac
      781 tctttcttta taaattgctt taatctaact gatatt
//
LOCUS       DAHHTG010000344          806 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.352, whole genome shotgun sequence.
ACCESSION   DAHHTG010000344 DAHHTG010000000
VERSION     DAHHTG010000344.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 806)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 806)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..806
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.352"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            complement(18..614)
                     /gene="fimE"
                     /locus_tag="NBI74_004739"
     CDS             complement(18..614)
                     /gene="fimE"
                     /locus_tag="NBI74_004739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418733.1"
                     /note="upgraded from regulatory protein to switch DNA
                     invertase; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria switch DNA invertase FimE"
                     /protein_id="HCD1934934.1"
                     /translation="MSKRRYLTGKEVQAMMQAVCYGATGARDYCLILLAYRHGMRISE
                     LLDLHYQDLDLNEGRINIRRLKNGFSTVHPLRFDEREAVERWTQERANWKGADRTDAI
                     FISRRGSRLSRQQAYRIIRDAGIEAGTVTQTHPHMLRHACGYELAERGADTRLIQDYL
                     GHRNIRHTVRYTASNAARFAGLWERNNLINEKLKREEV"
BASE COUNT          250 a          192 c          149 g          215 t
ORIGIN      
        1 attatcaata agttaaatca aacctcttct ctttttaatt tttcgtttat gagattattt
       61 ctttcccata atccggcaaa acgagcagca ttactggcgg tataacgcac agtatggcga
      121 atatttcgat gcccgagata atcctgaatt aaacgagtat ctgcaccacg ctccgccagt
      181 tcataaccgc aagcatgcct taacatatga ggatgagtct gcgttacggt tccagcttca
      241 ataccggcat cgcgaataat gcgataggcc tgctggcgag aaagccgact cccgcggcga
      301 gaaataaata tagcgtcagt ccggtcagcg cctttccagt tagcacgttc ctgggtccag
      361 cgttccacgg cttcacgctc atcaaaacgt aacgggtgaa cggtagaaaa tccgttcttc
      421 agtcggcgaa tatttattct accttcatta aggtcaaggt cctgataatg cagatcaagc
      481 agttcactaa tacgcatccc atgccgatat gccaacagaa taagacaata atctctggct
      541 cccgttgccc cgtaacaaac cgcctgcatc atggcctgaa cttctttacc ggtaagataa
      601 cgacgtttac tcacaatagt agtactccta actgagatat atttgaatga atacctatag
      661 gaaacctcaa tcggtcaaaa ttagcccaat agataaaaag ataaccacac acggagtgat
      721 gtggctatca gtcaattaca ttaaatatca tacaaaaata aaatatcact gatgtgacaa
      781 catctatttt cattgatttg attcaa
//
LOCUS       DAHHTG010000345          779 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.353, whole genome shotgun sequence.
ACCESSION   DAHHTG010000345 DAHHTG010000000
VERSION     DAHHTG010000345.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 779)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 779)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..779
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.353"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            71..763
                     /locus_tag="NBI74_004740"
     CDS             71..763
                     /locus_tag="NBI74_004740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001337214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCD1934935.1"
                     /translation="MKKETLADRLNLAMEQSGMSQGALAKASGVAQPTIWRLTSGNAR
                     GSTKIVEIANALGVRTEWLSSGIGPMRNDGQQLGKPTANHPKYFKIDVLDIEVSAGPG
                     VINREFVEVLRSVEYSFDDARHMFDGRKAENIRIINVRGDSMSGTIEPGDLLFVDITV
                     KSFDGDGIYAFLYDDTAHVKRLQMMKDKLLVISDNKSYSPWDPIEKDEMNRVFIFGKV
                     IGSMPQTYRKHG"
BASE COUNT          238 a          160 c          190 g          191 t
ORIGIN      
        1 atgcgtatta atcataacaa tagcaaaacg tgttttgacc atcaatacgc aagcgtataa
       61 attaaaactt atgaaaaaag aaactcttgc tgatcgctta aacctagcga tggaacaatc
      121 tggaatgtct caaggcgctc ttgcaaaggc gtctggcgta gctcaaccca caatctggag
      181 actgacaagc ggcaacgcgc gcggctcaac aaaaattgtt gaaatagcta atgcattggg
      241 tgttcgaaca gagtggctct catcaggcat aggcccgatg agaaatgacg gtcaacaatt
      301 agggaagcct actgccaacc atcccaaata cttcaagatt gacgttcttg atatagaagt
      361 gagtgccggg ccgggagtca tcaaccgtga gtttgtagaa gttctacgct cggttgagta
      421 ctcgtttgac gatgctcgtc acatgttcga tggtaggaag gcagaaaata tccgcatcat
      481 taacgtacgc ggtgacagca tgtcaggaac gattgaacct ggtgatcttc tattcgttga
      541 tatcacggtt aaatctttcg acggtgatgg tatctatgcg tttctgtacg acgacacagc
      601 ccatgtaaag cgcctgcaaa tgatgaagga taagctgctg gttatctctg ataacaaaag
      661 ctactcaccg tgggacccga tcgagaaaga tgagatgaac cgggtattta tcttcgggaa
      721 agttattggg agcatgccgc agacgtatag gaagcatgga tagtaccaat taaaaatta
//
LOCUS       DAHHTG010000346          764 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.354, whole genome shotgun sequence.
ACCESSION   DAHHTG010000346 DAHHTG010000000
VERSION     DAHHTG010000346.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 764)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 764)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..764
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.354"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            48..617
                     /locus_tag="NBI74_004741"
     CDS             48..617
                     /locus_tag="NBI74_004741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016158918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1934936.1"
                     /translation="MNKKLLAMGLLLSMTAGSAMAAANSGTTIGTGTLAVTGTVGTST
                     CSVTFPQSVTMPSITTAMYNQTSTQNAMVQQSAGNIQFSGCNGQSVNLKVMASNVTVA
                     GSKVIAYPRVNNNQQGWLGLTFGVSTDNGSSFKNLKLDNSPSEPISVTSDTFALPVRV
                     EAVKMSSNTATDIGQGNYSASFVVTATYA"
BASE COUNT          233 a          144 c          155 g          232 t
ORIGIN      
        1 ttaattaagt atttttttct tacaattatt gataatgggt ttctattatg aataaaaaac
       61 ttctcgcaat ggggctgtta ttaagcatga ccgcaggttc agctatggca gcagcaaatt
      121 ctggtacaac catcggaaca ggtacattag cggtaacagg cactgttggg acgtctactt
      181 gttcagtgac ttttccacaa agtgtgacaa tgccaagtat taccactgcg atgtacaatc
      241 aaacgtcgac ccagaatgcc atggttcaac agtctgctgg caatatccag ttttcaggat
      301 gcaatggtca atccgttaat cttaaagtga tggctagcaa tgttacggtt gccgggagta
      361 aagtaatcgc ttatccgaga gttaacaata accaacaagg gtggctaggt ctgacttttg
      421 gtgtatcaac agataacggc tcgtctttta agaatttgaa gttggacaat agcccatcag
      481 agccaatcag tgttacgagc gacacatttg cattacctgt gcgtgttgaa gcagttaaaa
      541 tgtcatcaaa tactgcaact gatataggtc aaggcaatta tagcgcatct tttgttgtaa
      601 ctgctacata cgcttaatgt tcattcattg ataaacaaag catggttgac gtacaaccat
      661 gcttttcatg gaataaaaat gagaaaaaaa atactttata cttttcttct cctgttatcg
      721 tcaccagttg ttgcacatga ccttgaaggt ataaatttag actt
//
LOCUS       DAHHTG010000347          762 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.355, whole genome shotgun sequence.
ACCESSION   DAHHTG010000347 DAHHTG010000000
VERSION     DAHHTG010000347.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 762)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 762)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..762
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.355"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            19..237
                     /locus_tag="NBI74_004742"
     CDS             19..237
                     /locus_tag="NBI74_004742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001340587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HCD1934937.1"
                     /translation="MLTVPPALLNALSLGTDNEVGMVIDNGRLIVEPYRRPQYSLAEL
                     LAQCDPNAEISAEEREWLDAPATGQEEI"
     gene            170..571
                     /gene="pemK"
                     /locus_tag="NBI74_004743"
     CDS             170..571
                     /gene="pemK"
                     /locus_tag="NBI74_004743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001398199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin
                     endoribonuclease PemK"
                     /protein_id="HCD1934938.1"
                     /translation="MLKYQLKNENGWMHRRLVRRKSDMERGEIWLVSLDPTAGHEQQG
                     TRPVLIVTPAAFNRVTRLPVVVPVTSGGNFARTAGFAVSLDGVGIRTTGVVRCDQPRT
                     IDMKARGGKRLERVPETIMNEVLGRLSTILT"
BASE COUNT          168 a          177 c          221 g          196 t
ORIGIN      
        1 agggttggcg gctcagttat gctgaccgtc ccaccggcac tgctgaatgc gctgtctctg
       61 ggcacagata atgaagttgg catggtcatt gataatggcc ggctgattgt tgagccgtac
      121 agacgcccgc aatattcact ggctgagcta ctggcacagt gtgatccgaa tgctgaaata
      181 tcagctgaag aacgagaatg gctggatgca ccggcgactg gtcaggagga aatctgacat
      241 ggaaagaggg gaaatctggc ttgtctcgct tgatcctacc gcaggtcatg agcagcaggg
      301 aacgcggccg gtgctgattg tcacaccggc ggcctttaat cgcgtgaccc gcctgcctgt
      361 tgttgtgccc gtaaccagcg gaggcaattt tgcccgcact gccggctttg cggtgtcgtt
      421 ggatggtgtt ggcatacgta ccacaggtgt tgtacgttgc gatcaacccc ggacaattga
      481 tatgaaagca cggggcggaa aacgactcga acgggttccg gagactatca tgaacgaagt
      541 tcttggccgc ctgtccacta ttctgacttg aacatggggt ttgaggggca actggatgaa
      601 aacgtacgat ttagggcact aaaaccgctg ttgtcccacc attctggtga ttcccaaacg
      661 ttatttggct aaaaagtagt tttgatgtgg tttattttcc gaattccaag cgcagcccta
      721 ctttcttgtg cgtatttgcg tgttttgcgc agttttgaag tt
//
LOCUS       DAHHTG010000348          744 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.356, whole genome shotgun sequence.
ACCESSION   DAHHTG010000348 DAHHTG010000000
VERSION     DAHHTG010000348.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 744)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 744)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..744
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.356"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            493..>744
                     /locus_tag="NBI74_004744"
     CDS             493..>744
                     /locus_tag="NBI74_004744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000990667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1934939.1"
                     /translation="MNADTWTRLQAFRHALELTSCEASLTAGYDHLKDFPAGCSELAS
                     QTLTDYLTEDGSNLYSCIVGMQWDNGPGRYGHVIADPARD"
BASE COUNT          199 a          196 c          198 g          151 t
ORIGIN      
        1 acgttccgaa aacaaaaaat acagataaac accaattaat tcagagcaat ctattgcgaa
       61 gaatcagagt aacagggccc catgatataa tcatggggcc ctgttgcttt tacagatgta
      121 aaaacgacca atcatttaac atctgttatc ggcaccgccc cggacgggtt cggcgcaaat
      181 atggtatggt accagcccgg caattcacct gccgtgcaat ctgacgaagc agtaccggcg
      241 tatgcagata tatcgtccag acatatctgc cgcattcagg cggtgagtaa agccttctct
      301 ctcaaaagag gagcaacagt tatccacaga ttctggggat aactgcgaaa agaatatgtg
      361 ccaggaaagt cggctcccgg gaaaaagacc gctggcggcg cacctgctgg atacgaaagc
      421 tgagctgaaa ggatcccggg aggacgtccg taaacttcag tccctggccc gtcatgacgg
      481 gaaggcgaaa aagtgaatgc ggacacctgg acccgcctgc aggccttccg acatgctctg
      541 gagctgactt cctgtgaggc ttctctcacc gccgggtatg atcacctgaa ggatttcccg
      601 gccggttgca gcgagctggc cagccagacg ctgactgatt atctgacgga agacggcagc
      661 aacctgtact cctgtattgt cggtatgcag tgggataatg gtcctggcag atacggccac
      721 gtcattgccg accctgccag ggac
//
LOCUS       DAHHTG010000349          742 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.357, whole genome shotgun sequence.
ACCESSION   DAHHTG010000349 DAHHTG010000000
VERSION     DAHHTG010000349.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 742)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 742)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..742
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.357"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            51..>742
                     /locus_tag="NBI74_004745"
     CDS             51..>742
                     /locus_tag="NBI74_004745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCD1934940.1"
                     /translation="MGRKWANIVAKKTAKDGATSKIYAKFGVEIYAAAKQGEPDPELN
                     TSLKFVIERAKQAQVPKHVIDKAIDKAKGGGDETFVQGRYEGFGPNGSMIIAETLTSN
                     VNRTIANVRTIFNKKGGNIGAAGSVSYMFDNTGVIVFKGTDPDHIFEILLEAEIDVRD
                     VTEEEGNIVIYTEPTDLHKGIAALKAAGITEFSTTELEMIAQSEVELSPEDLEIFEGL
                     VDALEDDDDVQKV"
BASE COUNT          248 a          124 c          164 g          206 t
ORIGIN      
        1 tgttaacgat ttaccagtaa tgtaaataaa ttttcgagga gatcattcca gtgggacgta
       61 aatgggccaa tattgttgct aaaaaaacgg ctaaagacgg tgcaacgtct aaaatttatg
      121 caaaattcgg tgtagaaatc tatgctgctg ccaaacaagg tgaacccgat ccagaattaa
      181 acacatcttt aaaattcgtt attgaacgtg caaagcaggc acaagttcca aagcacgtta
      241 ttgataaagc aattgataaa gccaaaggcg gtggagatga aacgttcgta cagggacgtt
      301 atgaaggctt tggtcctaat ggctcaatga ttattgctga gacattgact tcaaatgtta
      361 accgtacgat tgctaacgtt cgcacaattt tcaataaaaa aggcggcaat atcggagcgg
      421 caggttctgt cagctatatg tttgacaata cgggtgtgat tgtatttaaa ggaacagacc
      481 ctgaccatat ttttgaaatt ttgcttgaag ctgaaattga tgttcgtgat gtaactgaag
      541 aagaaggtaa tattgttatt tatactgaac ctactgacct tcataaagga atcgcggcgc
      601 taaaagcagc tggaatcact gagttctcaa caacagaatt agaaatgatt gctcaatctg
      661 aagttgagct ttccccagaa gatttagaaa tctttgaagg gcttgttgat gcccttgaag
      721 atgacgacga tgtacaaaaa gt
//
LOCUS       DAHHTG010000350          729 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.358, whole genome shotgun sequence.
ACCESSION   DAHHTG010000350 DAHHTG010000000
VERSION     DAHHTG010000350.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 729)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 729)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..729
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.358"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            <1..>729
                     /locus_tag="NBI74_004746"
     rRNA            <1..>729
                     /locus_tag="NBI74_004746"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          184 a          165 c          236 g          144 t
ORIGIN      
        1 ctagctggtc tgagaggatg accagccaca ctggaactga gacacggtcc agactcctac
       61 gggaggcagc agtggggaat attgcacaat gggcgcaagc ctgatgcagc catgccgcgt
      121 gtatgaagaa ggccttcggg ttgtaaagta ctttcagcgg ggaggaaggg agtaaagtta
      181 atacctttgc tcattgacgt tacccgcaga agaagcaccg gctaactccg tgccagcagc
      241 cgcggtaata cggagggtgc aagcgttaat cggaattact gggcgtaaag cgcacgcagg
      301 cggtttgtta agtcagatgt gaaatccccg ggctcaacct gggaactgca tctgatactg
      361 gcaagcttga gtctcgtaga ggggggtaga attccaggtg tagcggtgaa atgcgtagag
      421 atctggagga ataccggtgg cgaaggcggc cccctggacg aagactgacg ctcaggtgcg
      481 aaagcgtggg gagcaaacag gattagatac cctggtagtc cacgccgtaa acgatgtcga
      541 cttggaggtt gtgcccttga ggcgtggctt ccggagctaa cgcgttaagt cgaccgcctg
      601 gggagtacgg ccgcaaggtt aaaactcaaa tgaattgacg ggggcccgca caagcggtgg
      661 agcatgtggt ttaattcgat gcaacgcgaa gaaccttacc tggtcttgac atccacggaa
      721 gttttcaga
//
LOCUS       DAHHTG010000351          711 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.359, whole genome shotgun sequence.
ACCESSION   DAHHTG010000351 DAHHTG010000000
VERSION     DAHHTG010000351.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 711)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 711)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..711
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.359"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            <1..>711
                     /locus_tag="NBI74_004747"
     CDS             <1..>711
                     /locus_tag="NBI74_004747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000671528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HCD1934941.1"
                     /translation="KPGYVRLIQTRDYKTDAFPTYIPISSTKKFCDEFDIMIGRYGPP
                     IFQICRGLKGAYNVALLKVIPKEGVSRDFLYYFLKQDSVFQYVDKLSARTGGQTGVDL
                     VSLKEYPVRIPEEIECQEKLVTILSVIDKKIALNNRINTELEAMAKTLYDYWFVQFDF
                     PDANGKPYKTSGGKMEYNATLKREIPAGWNDSILGKFIELDRGVTYSKEDVRTQDDKD
                     TIGILRATNVTGNNVDIDD"
BASE COUNT          226 a          120 c          161 g          204 t
ORIGIN      
        1 aaacctggat atgtcaggtt gatccagacg cgtgattaca aaacggatgc atttcccacg
       61 tatataccaa tatcgtcgac gaagaaattt tgtgatgaat tcgatattat gattggtagg
      121 tatggtcccc caatttttca aatatgtcgc ggattgaaag gagcatataa cgttgccttg
      181 ctgaaggtta tacctaaaga aggtgtaagt cgtgattttt tatactattt tttgaagcaa
      241 gactctgttt tccaatatgt tgataaatta tctgccagaa ccggaggaca aactggagtt
      301 gatctagttt ctttaaaaga atatccagtc aggatacctg aagaaattga atgtcaagaa
      361 aaattggtga ctattcttag tgttattgat aaaaaaatcg ccctcaacaa ccgcatcaac
      421 acggaactgg aagcgatggc gaaaacactg tatgactact ggtttgtaca gtttgatttc
      481 cctgatgcca acggaaaacc gtataaaacc tccggcggga agatggagta taacgccacg
      541 ctgaaacggg aaattccggc aggttggaat gactccattc ttggtaagtt tattgagtta
      601 gatcgtggcg ttacttatag caaagaggat gttcgaactc aggatgataa agatacgata
      661 ggcattcttc gcgctacaaa tgtgacggga aataatgttg atattgatga t
//
LOCUS       DAHHTG010000352          704 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.360, whole genome shotgun sequence.
ACCESSION   DAHHTG010000352 DAHHTG010000000
VERSION     DAHHTG010000352.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 704)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 704)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..704
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.360"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            <1..>704
                     /locus_tag="NBI74_004748"
     CDS             <1..>704
                     /locus_tag="NBI74_004748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001152330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1934942.1"
                     /translation="IFANCPHETVTGYKYEKGKVLPIISRARFDAELYWSLGKYLKLF
                     VKDFYSLPFVSNIETIERNFSIFSFEWYRDHQDDPVVVKSMAHLLYSESYLGALREFA
                     PAITSESLALLKQGKPVGAFTRSIKMSLARSTPSIKKEYQEYLKNPKALSAKPSNAKQ
                     KVLPTNTSKVKIKEPAKINQHLAAQSSDSRKIRLLNKLERNPEMFFSDSNKKIVRLAG
                     KTAFNPILKPVLGNVI"
BASE COUNT          260 a          119 c          114 g          211 t
ORIGIN      
        1 attttcgcaa attgtccgca tgaaaccgtt actggttata aatatgaaaa agggaaagtt
       61 ttaccaatta tctcaagggc taggtttgat gcagaactat attggtctct tggcaaatac
      121 ttgaaattat ttgtcaaaga tttttatagt ttaccatttg ttagcaatat agaaacaatt
      181 gaacgcaatt tctctatttt ttcttttgaa tggtatagag atcatcagga tgatcctgtg
      241 gttgtaaaat cgatggccca tttattatac tctgaatcat atcttggagc attaagggaa
      301 tttgcaccgg caatcacttc agaatcttta gccttattaa aacaaggaaa accagttggt
      361 gcttttacaa ggtctattaa aatgagttta gcaagatcaa caccttcgat aaaaaaagaa
      421 tatcaagaat atcttaaaaa ccctaaagcc ttatctgcta aacctagtaa cgccaagcaa
      481 aaagtactac caactaatac ttcaaaagta aagataaaag aaccggctaa aattaatcaa
      541 catttagcag cacaaagttc tgacagcaga aagataagac ttctcaataa gcttgaacga
      601 aatcctgaaa tgttcttctc tgattcaaat aaaaaaattg ttagacttgc tggcaaaacg
      661 gcatttaacc caatattaaa gccagtgctg ggcaatgtaa taat
//
LOCUS       DAHHTG010000353          668 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.361, whole genome shotgun sequence.
ACCESSION   DAHHTG010000353 DAHHTG010000000
VERSION     DAHHTG010000353.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 668)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 668)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..668
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.361"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            <1..>668
                     /locus_tag="NBI74_004749"
     CDS             <1..>668
                     /locus_tag="NBI74_004749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000476153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-directed DNA polymerase"
                     /protein_id="HCD1934943.1"
                     /translation="FPSFNFGRVRGYFIANQNFKLHPNVATIIAQIACLDGSLPQGSP
                     CSPVITNLICRILDFRLSKLAVTYGCSYSRYADDITFSTNKKNIPDALVSNEKENEPG
                     KILVEEIHRAGFTLNHNKNRVSRCTSRQQVTGLTVNKKINVSREYIKNTRAMAHSLYF
                     EGSYTLIEKDGKHRKGTLSELEGRFAFIDMLDKYNNVEAKKNARPERYVVKGFGLDFK
                     QRLNS"
BASE COUNT          235 a          106 c          136 g          191 t
ORIGIN      
        1 ttcccatcct ttaattttgg tcgcgtgcga ggatatttta ttgcaaacca aaatttcaag
       61 ttacatccaa atgttgcaac cattattgcg cagatagcct gcctggatgg atcgcttccg
      121 caaggaagcc cttgttctcc agtaataact aatcttattt gtaggatttt agatttcaga
      181 ttatcaaagc tagcagtcac atatggttgt agttacagcc gctatgcaga tgacattacg
      241 ttttcaacaa acaaaaaaaa catccctgat gcattagttt ctaatgagaa agaaaacgaa
      301 ccaggtaaga tattggtaga agaaattcat cgtgcaggct tcactttaaa ccataataaa
      361 aacagagtgt ctaggtgtac atcaagacag caagttacag gtttaactgt aaataaaaaa
      421 ataaatgtaa gcagagagta tataaagaat acaagagcga tggcgcattc tttatacttt
      481 gaaggttcgt atacacttat tgagaaagat ggaaaacata gaaagggcac ccttagtgaa
      541 ttagaagggc gatttgcatt tatcgatatg cttgataaat ataataatgt ggaagcaaag
      601 aaaaatgcgc gtcctgagag atatgtggtt aaaggatttg ggttggattt taagcagaga
      661 cttaactc
//
LOCUS       DAHHTG010000354          656 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.362, whole genome shotgun sequence.
ACCESSION   DAHHTG010000354 DAHHTG010000000
VERSION     DAHHTG010000354.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 656)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 656)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..656
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.362"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            <1..>656
                     /locus_tag="NBI74_004750"
     CDS             <1..>656
                     /locus_tag="NBI74_004750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001546116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HCD1934944.1"
                     /translation="LLHDAVRPLINESIINNCIDALRNFEAVDVVIPSADTLVEVYDD
                     GCISNIPNRAIMRRGQTPQAFKLGTLKQAYQRAINAQRFSFTCDCGVVRSMVPGVRVA
                     TVVGAESNMKVTHPIDLFIAEKLLQEANKISFNTGDNLDYIKDKNIIIFGGNSGIGLE
                     IKKAAILLGANVEIASRSFNNVDIVDIECVKNYLAQMEKKLGCIDYIINTAGVLIKKP
                     "
BASE COUNT          207 a          100 c          137 g          212 t
ORIGIN      
        1 tctacttcat gatgcagtaa gaccattaat taatgaatca attattaata attgcattga
       61 tgctttaaga aattttgagg ctgtagatgt tgtaattcca tccgctgata cgctcgtcga
      121 agtgtatgac gatggatgta tttctaatat tccaaacagg gctataatgc gccggggtca
      181 gacccctcag gcatttaaat tagggacgct gaaacaggcc tatcaacgtg ccatcaatgc
      241 tcagcgattt agttttactt gtgattgtgg tgttgtacgc tcaatggtac ctggtgtacg
      301 tgtagcaact gtggttggtg ctgaatcaaa catgaaagtt acacacccaa ttgatctttt
      361 tattgctgag aagcttcttc aggaagcaaa taaaatttca tttaacactg gtgataatct
      421 cgattatatc aaagataaaa atataattat atttggtgga aacagcggga ttggtcttga
      481 gataaagaaa gcagctattt tattaggcgc gaatgttgaa attgctagcc gtagcttcaa
      541 taatgttgat attgttgata ttgagtgtgt aaaaaactat cttgctcaaa tggagaaaaa
      601 attaggttgc atcgattata tcattaatac tgctggagtt ttgataaaaa aaccta
//
LOCUS       DAHHTG010000355          656 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.363, whole genome shotgun sequence.
ACCESSION   DAHHTG010000355 DAHHTG010000000
VERSION     DAHHTG010000355.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 656)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 656)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..656
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.363"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
BASE COUNT          243 a          116 c          114 g          183 t
ORIGIN      
        1 ttcgctcaaa gattgaaaat caagagaaat tgttatagca gtgatacgag acaacaaaac
       61 ggaatgattg ccagccgtta gaaaacaccg gacactctcg tgtaaaaagc taccctgtct
      121 atggatatag cgatatcgac tcatcaagcg tggactgaat gatattcagc tccatatggg
      181 cggcaacctg gaggataaac atgatttcgc cgttggaatc aaggttgttc tgcaatatct
      241 accagactgc ttcaatttat ctgttttgga agtgcaataa tcttaattta actatttgaa
      301 gatgatgata ttgtcagatg ccagaaattt agtaatctgg agaaccgtgt ctatgatcac
      361 taaatattaa agtaatagtc gtcagtaaca catcgagtga tataacgtat tgacacctaa
      421 tcatataaaa acagatcgta attaagcagc ataagaatat gtcttattat cattaaaata
      481 cattctattt ccatcatcct taagataata aaacagtgag tgatggataa tttcacccca
      541 aaaaaaagca agcatataaa attaaaaaac atgactccac tttcattttt atgaaatggc
      601 atcgtgaaga ggcgccagcc aattataaag atcttgctcc tcaaaaaatg aaacaa
//
LOCUS       DAHHTG010000356          635 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.364, whole genome shotgun sequence.
ACCESSION   DAHHTG010000356 DAHHTG010000000
VERSION     DAHHTG010000356.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 635)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 635)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..635
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.364"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            complement(46..258)
                     /gene="cspI"
                     /locus_tag="NBI74_004751"
     CDS             complement(46..258)
                     /gene="cspI"
                     /locus_tag="NBI74_004751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock protein CspI"
                     /protein_id="HCD1934945.1"
                     /translation="MSNKMTGLVKWFNPEKGFGFITPKDGSKDVFVHFSAIQSNDFKT
                     LTENQEVEFGIENGPKGPAAVHVVAL"
BASE COUNT          222 a          121 c           98 g          194 t
ORIGIN      
        1 caacacgggc atctaaagtg aatatggttt gtaatattgt ctacctcaaa gcgccactac
       61 atgaacagcg gcaggacctt taggtccgtt ctcaatacca aattcaactt cctgattctc
      121 agttaatgtt ttgaaatcgt tgctctgaat tgctgagaaa tggacaaaca catctttgct
      181 gccatctttc ggcgtgatga aaccaaaacc tttttcaggg ttaaaccatt tcactaaacc
      241 agtcattttg ttagacataa ttattacctt ttgaagaaat tagcccttgg gcagaatggt
      301 ccgaaaaaaa atatcagaga gaaaaaccaa caaggaaatc tcaagaggta caaataataa
      361 aattataaca atgactgctt cagataaatt tgtaacaaac cagaacacca ttaacgcatg
      421 attaaccacc catagcaagg attacttttg taaagaaaaa cacagcaatg aaagaatagc
      481 tttatttatt aataaaacgt gtcattctga ttaagacctt ttatcttacc cttaagattt
      541 caggaatttt ggctcatgga agagtccttt ttatttaaat tttacattcc gcgatgtaaa
      601 tgttccgatt taatattacc ctacatttga tactt
//
LOCUS       DAHHTG010000357          618 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.365, whole genome shotgun sequence.
ACCESSION   DAHHTG010000357 DAHHTG010000000
VERSION     DAHHTG010000357.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 618)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 618)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..618
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.365"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            complement(115..603)
                     /locus_tag="NBI74_004752"
     CDS             complement(115..603)
                     /locus_tag="NBI74_004752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001543672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1934946.1"
                     /translation="MSSKRTVSAIPTHPEASVEEAKDWLTAEEFTHIEGMPGTARGAR
                     MRLEKLASLHPEIRRKRTAGKGVVYHISAATMSLDDERNRAKGEAFTLNDSDRQLSLW
                     VQLYRNMSPASRAQLMEQALRLVKDDLMSQGPNVSELAVQIMKLPEDERKRLFEKLNG
                     KV"
BASE COUNT          133 a          174 c          142 g          169 t
ORIGIN      
        1 gcaataggtt tttattcatt attaagtgat ggcatacttt ccattctgcc atgtcagaca
       61 ggtcaggatt ccggggtggt tccttctgtc tggcatggcg gaaaactgac gctatcacac
      121 cttcccgttc agcttttcaa agaggcgctt acgctcatct tcgggcagct tcatgatttg
      181 taccgccagt tcagaaacat tgggcccctg agacatcaga tcgtccttta ccagcctgag
      241 ggcctgctcc atcagttgcg cccgcgaagc tggcgacata ttgcgataca gctgcaccca
      301 gaggcttagc tggcgatcac tgtcgttgag agtaaacgct tcgcccttag ccctgtttct
      361 ctcatcatca agcgacattg tggctgcgct aatatggtaa accacacctt tcccggcagt
      421 ccttttacga cgtatttccg ggtgcaggga tgccagcttt tccaagcgca tcctggcgcc
      481 acgcgcagtg cccggcatcc cctcaatatg agtgaattcc tctgctgtca gccagtcttt
      541 agcctcttca acagaagctt caggatgagt aggtatagcc gaaactgttc tcttactgct
      601 catataaatt tcaccaga
//
LOCUS       DAHHTG010000358          605 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.366, whole genome shotgun sequence.
ACCESSION   DAHHTG010000358 DAHHTG010000000
VERSION     DAHHTG010000358.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 605)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 605)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..605
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.366"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
BASE COUNT          162 a          143 c          147 g          153 t
ORIGIN      
        1 cggaggcgtg ggcggtaata ggctgatttt caggtattac cggcttacaa cgtgtggttg
       61 atattgaggg gctgtgagtt tcgcgtattt gtcagaaacc cagatgagtt ttcctgtatg
      121 ggacgaaata aggcagcggc aatacctgga aaatttaata tggtatgaag gagaacctgt
      181 cggttacgct taatatacac ggtacactgt cagctcaggg gatacatgaa ccgttatctc
      241 accagctaca cagtatacac acactgacgc tgacagtagt cagcaggacc acaataatca
      301 gcgccagcag tatggtaacg atacggtctg tattccggta gtagccgaag gggtcgacag
      361 aaccacaata cgggcaacac actgtattat gagtaatcgc gtttccacaa tccctgcact
      421 tctttttgcg tatcaccatc actccctctc cacgccttat ccgtaacctg ttttttacat
      481 taaaaaaaca acgctgaaaa ttcaaccgtc agtccggaga cgaccgttcg ggttatcaca
      541 gagtgcctga gacagtgtcc tgccggaggt cacttcaggg actctgcgca ttttttacga
      601 cgtgg
//
LOCUS       DAHHTG010000359          604 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.367, whole genome shotgun sequence.
ACCESSION   DAHHTG010000359 DAHHTG010000000
VERSION     DAHHTG010000359.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 604)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 604)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..604
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.367"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            14..>604
                     /locus_tag="NBI74_004753"
     CDS             14..>604
                     /locus_tag="NBI74_004753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001686300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HCD1934947.1"
                     /translation="MLYDDKHKIEGFMYLKENDDANDINPALPPGRHLKIGTFKFESK
                     GTLRGQRFLKKAFDHAFSSKSDDIYVTVFDKHVHLIKLFQTYGFYIHGEKETHNGKEF
                     VYARSLHEPYGDILLDYPRIMTSRANKYLLAIYPEYHTRLFPDSKLVNESPDIVKDIS
                     HANSIHKIYICGMRSVMGMKRGDIIVIYRTGDKKGPA"
BASE COUNT          216 a          110 c          107 g          171 t
ORIGIN      
        1 agaatcagct tatgtgctct atgatgacaa gcataaaatc gaaggtttta tgtatctaaa
       61 agaaaatgat gatgcaaatg acattaatcc agcgctccca ccaggacgtc atctaaagat
      121 aggaacattc aaatttgaat ctaaaggcac ccttcgcgga caacgatttc taaaaaaagc
      181 gtttgaccat gcattttcat caaaatctga tgatatttat gttactgttt tcgacaaaca
      241 cgtccatcta ataaaacttt tccaaacgta cggattttac attcatggtg aaaaagaaac
      301 acataacggg aaagagtttg tatatgcgag gtctttgcat gagccttatg gtgatatttt
      361 attagattac cctcgaataa tgacatcaag ggccaacaaa tatttactgg cgatttatcc
      421 cgaatatcac actagactat tccctgattc aaaacttgta aatgaatcac cagatattgt
      481 caaagatata tcccatgcta acagcattca taaaatttac atatgtggaa tgcgttctgt
      541 gatgggaatg aaaagaggag atatcattgt catctataga accggagaca aaaaagggcc
      601 agct
//
LOCUS       DAHHTG010000360          601 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.368, whole genome shotgun sequence.
ACCESSION   DAHHTG010000360 DAHHTG010000000
VERSION     DAHHTG010000360.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 601)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 601)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..601
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.368"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            <1..579
                     /locus_tag="NBI74_004754"
     CDS             <1..579
                     /locus_tag="NBI74_004754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCD1934948.1"
                     /translation="KKLGFALSGLMLAMVAGSASADMDGGQLNISGLVVDNTCETRVD
                     GGNKDGLILLQTATVGEISEGVLDTTVGAKAKPFSITVDCSKANPAPGSTAKMTFGSV
                     FFGNSKGTLNNDMSINTPSDGVNIALHNIEGSTIKQVKINNPGDIYSKALDATSKSAT
                     YDFKASYVRADASLAATAGYVKTNTAYTVTYQ"
BASE COUNT          175 a          123 c          129 g          174 t
ORIGIN      
        1 aaaaaattag gttttgcgct ctctggtctt atgctggcaa tggttgcagg tagtgcttct
       61 gcagatatgg acggtggtca gttaaatatc agtggtctgg ttgttgataa tacctgtgaa
      121 acccgcgtag acggcggtaa taaagatggc ctgatcctgc tgcaaactgc aaccgttgga
      181 gaaatctctg agggtgtgct ggacactacc gttggtgcta aggctaaacc attcagcatt
      241 actgttgact gtagtaaagc taatccggca ccaggcagca cagcaaaaat gacttttggt
      301 tcagtcttct ttggtaacag taaaggaacc ctgaataatg acatgtcaat taatactcca
      361 tctgacggtg ttaatattgc tctgcacaat attgaaggtt ctactattaa gcaggttaaa
      421 atcaacaatc ctggcgatat ctattccaaa gcactggatg caacttccaa atccgcaact
      481 tatgatttta aagcgtctta tgttcgtgca gatgcaagtc tggcagccac agcaggttat
      541 gtaaaaacta acactgcata taccgttact tatcagtaat cacttccatt catattcata
      601 a
//
LOCUS       DAHHTG010000361          596 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.369, whole genome shotgun sequence.
ACCESSION   DAHHTG010000361 DAHHTG010000000
VERSION     DAHHTG010000361.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 596)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 596)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..596
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.369"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            17..596
                     /locus_tag="NBI74_004755"
                     /pseudo
     CDS             17..596
                     /locus_tag="NBI74_004755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
BASE COUNT          129 a          163 c          168 g          136 t
ORIGIN      
        1 tttttgaggt gctccagtgg cttctgtttc tatcagctgt ccctcctgtt cagctactga
       61 cggggtggtg cgtaacggca aaagcaccgc cggacatcag cgctatctct gctctcactg
      121 ccgtaaaaca tggcaactgc agttcactta caccgcttct caacccggta cgcaccagaa
      181 aatcattgat atggccatga atggcgttgg atgccgggca accgcccgca ttatgggcgt
      241 tggcctcaac acgattttcc gccatttaaa aaactcaggc cgcagtcggt aacctcgcgc
      301 atacagccgg gcagtgacgt catcgtctgc gcggaaatgg acgaacagtg gggatacgtc
      361 ggggctaaat cgcgccagcg ctggctgttt tacgcgtatg acaggctccg gaagacggtt
      421 gttgcgcacg tattcggtga acgcactctg gccacactgg agcgtcttct aagcctgctg
      481 tcggcctttg aggtcgtggt atggatgacg gatggctggc cgctgtatga atcccgcctg
      541 aagggaaagc tgcacgtaat cagcaagcgt tacactcagc gaattgagcg gcataa
//
LOCUS       DAHHTG010000362          594 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.370, whole genome shotgun sequence.
ACCESSION   DAHHTG010000362 DAHHTG010000000
VERSION     DAHHTG010000362.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 594)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 594)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..594
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.370"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            complement(<1..>594)
                     /locus_tag="NBI74_004756"
     CDS             complement(<1..>594)
                     /locus_tag="NBI74_004756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000834027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1934949.1"
                     /translation="KWDEMDVLLWKLGKNDSRRRSGVYYLNAYKDAYVQYNRDKIIKH
                     AYAAGIRPELLGGVAWIESGGMPENYKFQIYETKRMIGLLDMPENKTSFGSMGIQIRT
                     AAITLGLDPSELTTRNQLELATCLMEDDFTFQIAATYLRDLVLFDYPSSATLYMTNEQ
                     YIMAGIRYNRGVERDLGFFIYLINNLPARDTDDYKFIS"
BASE COUNT          189 a          124 c           90 g          191 t
ORIGIN      
        1 agagatgaat ttataatcat ctgtatcacg cgcaggcaaa ttattaatta aataaataaa
       61 aaaacccaaa tctcgttcaa caccacgatt atatctaata ccagccataa tatactgctc
      121 attcgtcata tacagtgttg ccgaagaggg ataatcaaaa aggacaagat ctcttaaata
      181 agtagcagca atttgaaagg taaaatcatc ctccattagg catgtcgcta actctaactg
      241 atttctggta gttaattctg aagggtctag acctagagta atcgctgcgg ttcgtatttg
      301 tatacccatt gagccaaatg atgttttatt ttcaggcata tctaacaatc cgatcattct
      361 ttttgtttca tatatctgaa acttataatt ttcaggcata cctcctgact caatccaggc
      421 cacgccgccc agtaactctg gacgaattcc agcggcgtat gcatgtttaa ttattttgtc
      481 tctattatat tgtacatatg catctttata agcatttaaa taatatactc ctgaacggcg
      541 acgcgaatca ttttttccta acttccagag taaaacgtcc atttcatccc attt
//
LOCUS       DAHHTG010000363          590 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.371, whole genome shotgun sequence.
ACCESSION   DAHHTG010000363 DAHHTG010000000
VERSION     DAHHTG010000363.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 590)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 590)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..590
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.371"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            <1..558
                     /locus_tag="NBI74_004757"
     CDS             <1..558
                     /locus_tag="NBI74_004757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
                     /protein_id="HCD1934950.1"
                     /translation="KIVLQWDEMIRTAGSLKLGKVQASVLVRSLLKSERPSGLTQAII
                     EVGRINKTLYLLNYIDDEDYRRRILTQLNRGESRHAVARAICHGQKGEIRKRYTDGQE
                     DQLGALGLVTNAVVLWNTIYMQAALDHLRAQGETLNDEDIARLSPLCHGHINMLGHYS
                     FTLAELVTKGHLRPLKEASEAENVA"
BASE COUNT          159 a          146 c          154 g          131 t
ORIGIN      
        1 aaaatagtcc ttcagtggga cgaaatgatc cggaccgctg gctccctgaa gctgggcaaa
       61 gtacaggctt cagtgctggt ccgttcattg ctgaaaagtg aacgtccttc cggactgact
      121 caggcaatca ttgaagtggg gcgcatcaac aaaacgctgt atctgcttaa ttatattgat
      181 gatgaagatt accgccggcg cattctgacc cagcttaatc ggggagaaag tcgccatgcc
      241 gttgccagag ccatctgtca cggtcaaaaa ggtgagataa gaaaacgata taccgacggt
      301 caggaagatc aactgggcgc actggggctg gtcactaacg ccgtcgtgtt atggaacact
      361 atttatatgc aggcagccct ggatcatctc cgggcgcagg gtgaaacact gaatgatgaa
      421 gatatcgcac gcctctcccc gctttgccac ggacatatca atatgctcgg ccattattcc
      481 ttcacgctgg cagaactggt gaccaaagga catctgagac cattaaaaga ggcgtcagag
      541 gcagaaaacg ttgcttaacg tgagttttcg ttccactgag cgtcagaccc
//
LOCUS       DAHHTG010000364          587 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.372, whole genome shotgun sequence.
ACCESSION   DAHHTG010000364 DAHHTG010000000
VERSION     DAHHTG010000364.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 587)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 587)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..587
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.372"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
BASE COUNT          141 a          160 c          145 g          141 t
ORIGIN      
        1 acagaggggc aggcgttcag gcattaccgg cctgtatctt ctgaagggga ctcgtatttg
       61 tcggggctga gtattccgct cgattcgatc acggattggc ggaatacctg actgtgattg
      121 attcaggtac cattttgcag ggagccggtg cgttactgtc tggcacaaca aacgcagcgg
      181 gtcgctctgc cccgccgatt cctgataatc cctcccctgt aacctactgc aacagtacaa
      241 atacactgac gcctgaaacg gtaaccagaa tcagcaccag cagtaacgtc agtaaacaga
      301 gcagacgatc ggtcttccgg taataaccga aggggtcgac agcgccacaa tacgggcaac
      361 atactgtatt atgagtaatt gcgttaccac agtccctgca cttctttttg catatcacga
      421 tcgctccctc tccacgcctt atccgtaacc tgttttttac attaaaaaaa caacgccggg
      481 aattcaatcg tcagtccggc gatgaccgtt cgggttatca cagagtgcct gagacagtgt
      541 cctgccggag gtcacctcag gggctctgcg cattttttac gacgtgg
//
LOCUS       DAHHTG010000365          582 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.373, whole genome shotgun sequence.
ACCESSION   DAHHTG010000365 DAHHTG010000000
VERSION     DAHHTG010000365.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 582)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 582)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..582
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.373"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            complement(<1..380)
                     /locus_tag="NBI74_004758"
     CDS             complement(<1..380)
                     /locus_tag="NBI74_004758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ZinT/AdcA family metal-binding protein"
                     /protein_id="HCD1934951.1"
                     /translation="MAIRLHKLAVALGVFIVSAPAFSHGHHSHGKPLTEVEQKAANGV
                     FDDTNVQNRTLSDWDGVWQSVYPLLQSGKLDPVFQKKADADKTKPFAEIKDYYRKGYA
                     TDIEMIGIEDGIVEFHRNNETTSC"
BASE COUNT          180 a          106 c          118 g          178 t
ORIGIN      
        1 ttacaggatg ttgtttcatt atttctatgg aattcgacaa tgccgtcctc aatgccaatc
       61 atttcgatat ctgtcgcata acctttgcga taataatctt taatttcagc aaatggtttg
      121 gttttatctg catccgcttt cttctgaaag acgggatcaa gtttgccact ttgcagtaaa
      181 gggtaaacgg attgccagac tccatcccag tcactgagcg ttcggttttg tacattggta
      241 tcatcaaaaa caccattagc agctttttgt tcgacctctg ttaagggttt gccgtgtgag
      301 tgatgaccat gcgaaaaggc aggagcgcta acaataaaga cacctaaagc aacagccagt
      361 ttatgaagac gaatcgccaa aacaatttcc tccagagtta atgtacatga tgtgtaatgt
      421 tatattgtaa catatgaaaa aatgcaacaa ctcattagga aatgagagca tggtattctc
      481 aggtgtattt aactatctta ttgaaataaa ataaattatt ctggtgatga aaggttgatg
      541 acaaacgaaa cataacttga tgcgatctgt gtgggccgga ta
//
LOCUS       DAHHTG010000366          574 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.374, whole genome shotgun sequence.
ACCESSION   DAHHTG010000366 DAHHTG010000000
VERSION     DAHHTG010000366.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 574)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 574)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..574
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.374"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            127..363
                     /locus_tag="NBI74_004759"
     CDS             127..363
                     /locus_tag="NBI74_004759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component-system connector protein YcgZ"
                     /protein_id="HCD1934952.1"
                     /translation="MHQNSVTSDSAGAITRYFAKANLPTQQETLGEIVTEILKDGRNL
                     SRKSLCAKLLCRLEQATGEEEQKHYNALIGLLFE"
     gene            406..>574
                     /locus_tag="NBI74_004760"
     CDS             406..>574
                     /locus_tag="NBI74_004760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component-system connector protein YmgA"
                     /protein_id="HCD1934953.1"
                     /translation="MKTSDNESIKYEITGQAVLQILRMKINFSLQTLIKQLLVMKSAE
                     ENTFRRDLIDSI"
BASE COUNT          187 a           98 c          115 g          174 t
ORIGIN      
        1 gcattgtttt atatgaatta gcactaattg cgaaaaatta atttatcatt ctgtacacat
       61 atttcgtaca agtttgctat tgttacctca cttaacattg attaacattt ttaacagagg
      121 cgtagcatgc atcaaaattc agtgacttca gattctgcag gagcaatcac tcgttacttt
      181 gcaaaagcta acttgcctac tcagcaggaa actctcgggg aaattgtgac tgagattttg
      241 aaagatggac gtaatctgag tcgaaagtcg ctttgtgcca aacttctttg ccgactggaa
      301 caagcgacag gggaagagga acagaaacat tataatgcac taattgggtt gctttttgaa
      361 taatgcaaca gcctgattat ttaacaggtt agctatgaag tcgttatgaa gacatctgat
      421 aatgaaagta ttaaatatga aattactggc caggcggtgc tccagatact gcgcatgaag
      481 ataaattttt cgttgcagac tcttattaag caattactcg taatgaaatc ggctgaggaa
      541 aatactttcc gacgagattt aattgacagc ataa
//
LOCUS       DAHHTG010000367          572 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.375, whole genome shotgun sequence.
ACCESSION   DAHHTG010000367 DAHHTG010000000
VERSION     DAHHTG010000367.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 572)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 572)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..572
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.375"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            142..561
                     /locus_tag="NBI74_004761"
     CDS             142..561
                     /locus_tag="NBI74_004761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001423036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1934954.1"
                     /translation="MYAKSFLALDGNGRLTGARTAQTAPYAYYTCHLCGSALRYHPQH
                     DTERPWFEHTDDGLTEHGHECPYVQPEHREVQLIKRLQQFVPDALPVVRKACWYCRQC
                     RHAYYGERYCTHCQTGRFSEDGEQNERYKNGAGDHAG"
BASE COUNT          139 a          165 c          151 g          117 t
ORIGIN      
        1 tcagtgtgat gacgtgttca cagcgaccac actgattttc ccttctgaga ctgaactgaa
       61 aaccgccatt cacacctgac cacgctgccc gtaccgggac ggctgttgcc tgcgtgtcgt
      121 ctggcggtaa ggaaccacac tatgtacgca aaatcttttc tcgcacttga tggcaacgga
      181 cgtctgaccg gtgcccgtac cgcacagacc gcaccttatg cttactacac ctgccacctt
      241 tgcggcagtg cactcagata ccacccgcag cacgacacgg aacgtccctg gtttgaacac
      301 actgacgacg ggctgacgga acacggtcat gagtgccctt atgtacaacc ggaacacagg
      361 gaagtacagc tgattaaacg tctgcagcaa ttcgtaccgg atgccttacc cgtggtgcgt
      421 aaagcctgct ggtactgcag acaatgtcgc cacgcttatt atggggaacg ctattgtaca
      481 cactgccaga ccgggcgctt cagtgaggac ggggagcaga atgaacggta taaaaacggg
      541 gctggggatc acgccgggtg agcacattat ca
//
LOCUS       DAHHTG010000368          561 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.376, whole genome shotgun sequence.
ACCESSION   DAHHTG010000368 DAHHTG010000000
VERSION     DAHHTG010000368.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 561)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 561)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..561
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.376"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            429..509
                     /gene="shoB"
                     /locus_tag="NBI74_004762"
     CDS             429..509
                     /gene="shoB"
                     /locus_tag="NBI74_004762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin ShoB"
                     /protein_id="HCD1934955.1"
                     /translation="MTDCRYLIKRVIKIIIAVLQLLLLFL"
BASE COUNT          166 a          114 c          115 g          166 t
ORIGIN      
        1 aaagttaaaa taataccgtt ctctttataa agtaaaaccc ccgaactggt aaggaacggg
       61 ggttttactt tttaacttta attttgtgca gcacacgatt ccaacgaaaa tgattatgga
      121 tcaatttcgt tgacctgtac aagcaacttg caatctctga cataaaaaac gcgagccttt
      181 acgaagaaat cgacattgat gaaaagatta acgtgaccgc tttttgataa gtacattaaa
      241 atcggtttcg ttattggcaa ttttgctgtg ctttacacca tgccactgaa ttcccccatt
      301 gaaacgagtg gtgtcgtcaa agctctggtg tggagtgcag cactcgattc caataactcg
      361 cacgttcagt tttggggaga tgtaagggct aatctgaacg gctgcattcc ttgtttaagg
      421 aaaaacgaat gactgattgc cgatacctga ttaaacgggt catcaaaatc atcattgctg
      481 ttttacagct gctccttttg ttcttataac acaaggaaac gtacttaagg tgcgtccggt
      541 gaaccaatcg gacgcgcctt t
//
LOCUS       DAHHTG010000369          557 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.377, whole genome shotgun sequence.
ACCESSION   DAHHTG010000369 DAHHTG010000000
VERSION     DAHHTG010000369.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 557)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 557)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..557
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.377"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
BASE COUNT          175 a          123 c           94 g          165 t
ORIGIN      
        1 gtaaaatcaa cataaaaaat tcagcaaaat ttaatattcg ttcaactagc cgtctcacat
       61 atgacattat tagtcaactc catttaccac aaaatggcta tttcatggaa cgcagtaatt
      121 tcttagtggc tattatcaag ctaattctta ggtccctagc gattattcct ggctggttcg
      181 tcagtttagg tcatgagatt gttacattcc tttttatcct ggagatatgg gggaacgcag
      241 gcgacgcttt ttcatctaca tccgttggta aatatggctt tggtcactac gacttaaacc
      301 ctcctcaatc acagctactc gccgggcgat atggcaaaca aactaattga aacatcatcc
      361 tgaaaacacc agcatcaaca agcctctcca gatcgacatc aggagtgacc agttacaagc
      421 cacaaaccag gaaacatatt tcatgatgag aattatgctc agcctgatgg cgggtaaaca
      481 cttattttca ccaagctcac tgagttagct tgcaaagctt tctttgttta gattctaaat
      541 aaattattta taaaaca
//
LOCUS       DAHHTG010000370          540 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.378, whole genome shotgun sequence.
ACCESSION   DAHHTG010000370 DAHHTG010000000
VERSION     DAHHTG010000370.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 540)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 540)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..540
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.378"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
BASE COUNT          162 a          117 c          109 g          152 t
ORIGIN      
        1 tgctgttgtg cagggaatag tcaggggata ttccatagaa ctgaacgttc atcccccaga
       61 atgagaagtt gaacatgcct ccatagcgaa tagtgttccg gactcgaatc gttgaacgat
      121 atcctgcaat ttggagcatg aggtgtttct atcctatgca acagaatcat cattaatcca
      181 gatgtaggcc tgaaaaacag gcctggcaat atcgatacca acaatcctga tcgtgttcat
      241 gggatgttac tctcggatta atgcagtcag taaaagtatg gcactgactg cattaagaag
      301 ggacgtccat catatcccga cgcaaacgct cccaccgaca tcgccattga gtcctgcacc
      361 gtatcggtga acttgtaact atcccgtata atcattctat acacatttat tggcttgatg
      421 agtcaaatac ttcttaattt cggagagaag tgtgtcaggt gatacgttaa ctcgccatta
      481 tctacatatt tagagaatta ctcaaataat aacgaattat ttacccatgt gctcaattaa
//
LOCUS       DAHHTG010000371          519 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.379, whole genome shotgun sequence.
ACCESSION   DAHHTG010000371 DAHHTG010000000
VERSION     DAHHTG010000371.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 519)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 519)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..519
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.379"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            61..>519
                     /locus_tag="NBI74_004763"
     CDS             61..>519
                     /locus_tag="NBI74_004763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000643690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HCD1934956.1"
                     /translation="MKAIDLFAGAGGFSLSAHNTGAIDVVAAIEFDSAAANTYRKNML
                     ERLEHKTELLQEDILLVGPKKLRKKIKLKKGELDMILGGPPCQGFSSHRINDAGVDDP
                     RNKLLLRYFDFVCEFKPKAFLVENVSGLLWKRHEAHLKRFKFLASKNGYTL"
BASE COUNT          171 a           76 c          111 g          161 t
ORIGIN      
        1 gttgcatttc taaaaaaaaa gagtaaaata tcaaaattta ggagttactt ttggacttat
       61 atgaaggcaa ttgacttatt tgcgggggca ggagggttta gtttatccgc ccacaataca
      121 ggcgctatag atgttgttgc tgctatagaa ttcgatagcg cggctgcaaa cacctacaga
      181 aaaaatatgt tagaaaggct tgagcataag accgaacttt tacaggaaga tattttactc
      241 gtaggcccaa aaaagttaag aaaaaaaata aagctcaaga aaggcgagct tgatatgata
      301 cttggtggac ctccgtgcca aggtttttcc agtcatcgaa ttaatgatgc tggtgttgat
      361 gatcctagaa ataaattact tttaaggtat ttcgattttg tttgtgaatt taaaccaaaa
      421 gcttttttgg tagaaaatgt ctccggtttg ttatggaaga gacatgaagc ccatttgaaa
      481 cgctttaagt ttttggcttc caaaaatggt tatacttta
//
LOCUS       DAHHTG010000372          512 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.380, whole genome shotgun sequence.
ACCESSION   DAHHTG010000372 DAHHTG010000000
VERSION     DAHHTG010000372.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 512)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 512)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..512
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.380"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            <1..207
                     /locus_tag="NBI74_004764"
     CDS             <1..207
                     /locus_tag="NBI74_004764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000764086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1934957.1"
                     /translation="DKIFDEKENIKIGTEYLQYWIDKEATIEEAYRTYRGTDEAYYSV
                     IKPCAEKLAKDPDNIQILMEGIGR"
BASE COUNT          173 a           73 c          101 g          165 t
ORIGIN      
        1 gataaaatat ttgatgaaaa agaaaatatt aagattggca cagagtattt gcaatactgg
       61 atagataaag aagcaacaat tgaggaggca tatcggacat atcgtggaac agatgaagca
      121 tactattctg ttataaaacc ttgcgctgaa aaactggcta aagatcctga taacatacaa
      181 atactaatgg agggaatagg aagatagtat gcgcattatg ttaatattac tttgtttttt
      241 tctttcccct gtttttcagg cgataagatt aaaatcgtga aaacctgtca attgaaaaaa
      301 aatgaaagtg tgcagttgct ttcccttaga accattgacg gtgatacatt atatctaaaa
      361 ttcagaaatg ttatcaccgg ggccttttta gatactccaa ctaaaagtta tgattttttt
      421 ggagatgtgg ttctttctca gtgcataaat ggttctctta tctttgcgct ggaatatggt
      481 tctccatata taaaaggttg tctggtaact gg
//
LOCUS       DAHHTG010000373          507 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.381, whole genome shotgun sequence.
ACCESSION   DAHHTG010000373 DAHHTG010000000
VERSION     DAHHTG010000373.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 507)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 507)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..507
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.381"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            complement(101..310)
                     /gene="fumD"
                     /locus_tag="NBI74_004765"
     CDS             complement(101..310)
                     /gene="fumD"
                     /locus_tag="NBI74_004765"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase FumD"
                     /protein_id="HCD1934958.1"
                     /translation="MGNRTKEDELYREMCRVVGKVVLEMRDLGQEPKHIVIAGVLRTA
                     LANKRIQRSELEKQAMETVINALVK"
BASE COUNT          149 a          125 c           79 g          154 t
ORIGIN      
        1 gaaattcgta taatttcagc agttgttatt tagaatgatt aactggaccg caactgaact
       61 aacaatatca ggatgaagct taccgcctca tcctgaatgg tcattttact agtgcgttaa
      121 tgactgtttc catcgcctgc ttttccagct cactgcgctg aatgcgttta ttggctaatg
      181 ctgtacgtaa tacgcctgcg ataacaatat gctttggttc ctgccccaga tcgcgcattt
      241 ccagcacaac tttaccgaca actctgcaca tttctcggta cagttcatcc tcttttgtcc
      301 ggtttcccat tctttatacc tatttatcat gccaactatc agcatatatc aatctaacca
      361 attaaacaaa aatcaaacaa aatcagacaa ataacgtgat aaattatttt aactgtagca
      421 attgagcgat gatgtattta tacaccggat gaactttcac ttatcctcac actgacaacc
      481 ccggcaccag acgttgcgca aacaacg
//
LOCUS       DAHHTG010000374          498 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.382, whole genome shotgun sequence.
ACCESSION   DAHHTG010000374 DAHHTG010000000
VERSION     DAHHTG010000374.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 498)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 498)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..498
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.382"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            complement(235..>498)
                     /locus_tag="NBI74_004766"
     CDS             complement(235..>498)
                     /locus_tag="NBI74_004766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001383102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1934959.1"
                     /translation="VKVDDGVTGYINNASVMTSGDGSYGISVDSQNKVLYISDSNIKT
                     SGSVSDKENGGITASAVVSEFGGTIVMNGDNSVETRGHIQRDF"
BASE COUNT          140 a          129 c           69 g          160 t
ORIGIN      
        1 taccattagt tgttatggaa ccaccattca tttttaaatc agcacggcta ataacttcgt
       61 aactgttaga atcacaaact tcatcatcta cagcatcgac acatgttcga gactctccag
      121 gacttgaaca tgcaagaact cctactgcat tttccccata ggtaacaatg ttcgttttat
      181 ctgtggtttc aagactggtg ttattttcca cagtcccaga atcattaacc tgacttaaaa
      241 atcccgctga atatgccccc tcgtctcgac tgaattatca ccattcataa cgatggtgcc
      301 accaaattca ctgactactg cgctggctgt tatccctcca ttttctttgt cagatacgct
      361 tccagaggtc ttaatattgc tatcgcttat atataatact ttgttttggc tatcaacaga
      421 aataccataa gatccatcac cactagtcat cacactggca ttatttatat atccagttac
      481 accgtcatcc actttcac
//
LOCUS       DAHHTG010000375          497 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.383, whole genome shotgun sequence.
ACCESSION   DAHHTG010000375 DAHHTG010000000
VERSION     DAHHTG010000375.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 497)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 497)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..497
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.383"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            <1..>497
                     /locus_tag="NBI74_004767"
     CDS             <1..>497
                     /locus_tag="NBI74_004767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001046280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1934960.1"
                     /translation="LKQIIDTYSSTVCNNIVVNEIDLSSIVDTDLRNIFAVHLYDFDG
                     NYKKTGLEYAGLEKVRVSRIKTSLDINGDNLEGNNNEETQELLNKISPSTDNENDDDI
                     NNQEMASDDDENLTFNINNAAYDGLSLVNAPQIKELCSDGFYRSLIRWKSFSSVVKNH
                     TITFE"
BASE COUNT          188 a           71 c           87 g          151 t
ORIGIN      
        1 ttctcaagca aataattgat acatattcat caacagtttg taacaacata gttgtcaacg
       61 aaatagattt atcaagtatc gttgatacag atcttaggaa tatttttgct gttcatcttt
      121 atgattttga tggaaattat aaaaaaacag gacttgaata tgctggtctt gaaaaggtta
      181 gagtaagcag aataaaaaca tctctggata ttaatggaga taatctcgaa ggaaataata
      241 acgaagaaac acaagagtta ttaaacaaga tttcaccttc tacagataac gaaaatgatg
      301 atgacattaa taatcaggaa atggcatctg acgatgatga aaacctaact tttaacatta
      361 acaatgccgc gtatgatggc ctatcattag tgaacgcacc tcaaattaag gaactgtgca
      421 gtgatgggtt ttatcgaagc ttaattcgat ggaagtcatt ttcctcagta gttaagaatc
      481 atactataac ttttgaa
//
LOCUS       DAHHTG010000376          484 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.384, whole genome shotgun sequence.
ACCESSION   DAHHTG010000376 DAHHTG010000000
VERSION     DAHHTG010000376.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 484)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 484)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..484
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.384"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            <1..484
                     /locus_tag="NBI74_004768"
     rRNA            <1..484
                     /locus_tag="NBI74_004768"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          119 a          119 c          143 g          103 t
ORIGIN      
        1 gctgtcgtca gctcgtgttg tgaaatgttg ggttaagtcc cgcaacgagc gcaaccctta
       61 tcctttgttg ccagcggtcc ggccgggaac tcaaaggaga ctgccagtga taaactggag
      121 gaaggtgggg atgacgtcaa gtcatcatgg cccttacgac cagggctaca cacgtgctac
      181 aatggcgcat acaaagagaa gcgacctcgc gagagcaagc ggacctcata aagtgcgtcg
      241 tagtccggat tggagtctgc aactcgactc catgaagtcg gaatcgctag taatcgtgga
      301 tcagaatgcc acggtgaata cgttcccggg ccttgtacac accgcccgtc acaccatggg
      361 agtgggttgc aaaagaagta ggtagcttaa ccttcgggag ggcgcttacc actttgtgat
      421 tcatgactgg ggtgaagtcg taacaaggta accgtagggg aacctgcggt tggatcacct
      481 cctt
//
LOCUS       DAHHTG010000377          449 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.385, whole genome shotgun sequence.
ACCESSION   DAHHTG010000377 DAHHTG010000000
VERSION     DAHHTG010000377.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 449)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 449)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..449
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.385"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            <1..>449
                     /gene="waaZ"
                     /locus_tag="NBI74_004769"
     CDS             <1..>449
                     /gene="waaZ"
                     /locus_tag="NBI74_004769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="3-deoxy-D-manno-oct-2-ulosonate III transferase
                     WaaZ"
                     /protein_id="HCD1934961.1"
                     /translation="RDDFYKFSQRSRYTIVNVDVYEHASKEDKLYILQNCLVLRSFYR
                     REKGGFIKKIKFNILSQIHKELLISVPLSKKGRLVGFCKDISFGYCSCHTIAFAAIQI
                     AYSLKYARIICSGLDLTGNCSRFYDENNNPMPSELSKDLFKILPFFR"
BASE COUNT          130 a           78 c           83 g          158 t
ORIGIN      
        1 tcgtgatgat ttttataaat ttagccagag aagccgttat accatagtaa acgttgacgt
       61 ttatgaacat gcttccaaag aagataaact ttatatcctc cagaactgcc tcgtattacg
      121 gtccttttat cgccgggaaa aaggtggttt tataaagaag attaaattta atatattgag
      181 tcagattcac aaagaactgc tgatctctgt accgttgtct aaaaaaggtc gtctggttgg
      241 attttgcaag gacattagtt ttggttattg ttcatgccat actattgcct ttgctgcaat
      301 tcaaatagca tattcactta agtatgcacg gattatttgt tctggtcttg atttaacggg
      361 taactgctct cgtttctatg acgaaaataa taatcccatg ccctcggaat tgagtaagga
      421 tttattcaaa atcttgccat ttttccgtt
//
LOCUS       DAHHTG010000378          445 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.386, whole genome shotgun sequence.
ACCESSION   DAHHTG010000378 DAHHTG010000000
VERSION     DAHHTG010000378.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 445)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 445)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..445
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.386"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            1..445
                     /locus_tag="NBI74_004770"
                     /pseudo
     CDS             1..445
                     /locus_tag="NBI74_004770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310454.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="ShET2/EspL2 family type III secretion system
                     effector toxin"
BASE COUNT          137 a          101 c           81 g          126 t
ORIGIN      
        1 tggaaaccac gcctattaaa gctaaccatt tgcgaagcat actggattat attgaacggg
       61 aacagttaac tgccgaaaaa cgtaaccatt gcatgaaact gtccaaaaaa attcatagag
      121 aaaaaaccat acagccaaca gtaaatctca atgatagtgt attttttcgc aatctccttc
      181 tgacactatt ttttgtcgcc atccgtcatt acaatacgcc cttgattcat tgagaaatgg
      241 aaaaggcaaa gtcaacctga ttaaacatta ctcctccatt gaatccatac agcagcatgt
      301 tcccttagtc cgggacgcgg aattcagagc attacttcgc catcctcttg caaggagtcg
      361 cgttatcgcg agtcaggatt ttggctttgc tttagatatt ttcttctgtc gaatgatggc
      421 aaacaatgtc agtcatatgt ccgcg
//
LOCUS       DAHHTG010000379          443 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.387, whole genome shotgun sequence.
ACCESSION   DAHHTG010000379 DAHHTG010000000
VERSION     DAHHTG010000379.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 443)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 443)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..443
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.387"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            <1..>443
                     /locus_tag="NBI74_004771"
     CDS             <1..>443
                     /locus_tag="NBI74_004771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CHASE9 sensor domain-containing protein"
                     /protein_id="HCD1934962.1"
                     /translation="KMEMMPFLDSVGLVLDDNKYYLFSRRANDKIVVYHQEQVNGPLV
                     DESGRVIFADFNPSKRPWSMASDDSNNSWNPAYNCFDRPGKKCISFTLHINGKDHDLL
                     AVDKIHVDLNWRYLNEYLDHISANDEVLFLKQGHEIIAKNQLARE"
BASE COUNT          134 a           81 c           99 g          129 t
ORIGIN      
        1 gaaaatggaa atgatgccat ttttagactc ggtgggactg gtacttgatg ataataaata
       61 ttatctcttt tcgcggaggg cgaatgataa aatcgttgtt tatcatcagg aacaagtaaa
      121 tggaccgctt gtcgacgagt cagggcgggt tatttttgcc gattttaacc catcgaaacg
      181 accgtggtcg atggcttcag atgactctaa caacagctgg aacccggcat acaattgctt
      241 tgatcgtccg ggtaaaaaat gtatctcttt tacgctacac atcaacggca aagatcatga
      301 tttgttagcg gtagataaaa ttcatgtcga tttaaactgg cgatatctga acgagtatct
      361 tgatcacatc agcgctaatg atgaagttct atttttaaag caaggtcatg agatcattgc
      421 taagaatcaa ctcgctcgtg aaa
//
LOCUS       DAHHTG010000380          426 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.388, whole genome shotgun sequence.
ACCESSION   DAHHTG010000380 DAHHTG010000000
VERSION     DAHHTG010000380.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 426)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 426)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..426
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.388"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            96..287
                     /locus_tag="NBI74_004772"
     CDS             96..287
                     /locus_tag="NBI74_004772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2633 family protein"
                     /protein_id="HCD1934963.1"
                     /translation="MSQATSMRKRHRFNSRMTRIVLLISFIFFFGRFIYSSVGAWQHH
                     QSKKEAQQSTLSVESPVQR"
BASE COUNT          103 a          113 c           84 g          126 t
ORIGIN      
        1 cgcttcttta cataacgaag aattgaccat tttgtcctgt tgtgccttaa tgtaagtacc
       61 gtccacggcg tgggacatac ttcaaggaac cttttgtgag tcaggcaacc agtatgcgaa
      121 aacgacaccg atttaacagt cgcatgaccc gtatcgtact gctcatcagc tttatcttct
      181 tctttggccg ttttatctac tcgtccgtcg gtgcctggca gcaccatcag agcaaaaaag
      241 aagctcagca atccacactc tccgtcgaat caccggtaca acgttagcgg ttaccttctc
      301 cactctcaca gaacataacg gcacctcgct gtcggatgct tttgctcttt gagattatca
      361 aagcggcaga tattctttca tcttaaattt tacgtctttg ttctgactgc agttcggctg
      421 cgaaat
//
LOCUS       DAHHTG010000381          418 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.389, whole genome shotgun sequence.
ACCESSION   DAHHTG010000381 DAHHTG010000000
VERSION     DAHHTG010000381.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 418)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 418)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..418
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.389"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
BASE COUNT          123 a           75 c           84 g          136 t
ORIGIN      
        1 gctagcccca aaaacggtaa atttagctta agtgcataat tggttagctc aaaagcatta
       61 tttttcattt aaataaatta gttaattggt cttgtttaga tgattcaact gggctgacta
      121 ctttctttgt atatactccg gataaatttt cccagctaac ttgcctaatc atcactctga
      181 tgccagaaat gaacagaacg caaaccatct ataacttatt gaggattttg aaaaaaattg
      241 attgggggct tgagttatat gatgactatg ctaatttaat acggcacatg caggtagatt
      301 tgttggttgt ggtatcgcaa tcagtgttaa caaggtcggg agtattcgcc ctctgactgc
      361 cgtcaagtca tcttggcgtc accgttaaat gcgtaagagt acctgcatgt gcattaac
//
LOCUS       DAHHTG010000382          416 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.390, whole genome shotgun sequence.
ACCESSION   DAHHTG010000382 DAHHTG010000000
VERSION     DAHHTG010000382.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 416)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 416)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..416
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.390"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            43..>416
                     /locus_tag="NBI74_004773"
     CDS             43..>416
                     /locus_tag="NBI74_004773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS26 family transposase"
                     /protein_id="HCD1934964.1"
                     /translation="MNPFKGRHFQRDIILWAVRWYCKYGISYRELQEMLAERGVNVDH
                     STIYRWVQRYAPEMEKRLRWYWRNPSDLCPWHMDETYVKVNGRWAYLYRAVDSRGRTV
                     DFYLSSRRNSKAAYRFLGKILN"
BASE COUNT           92 a          111 c          111 g          102 t
ORIGIN      
        1 tggtgataaa cttatcatcc ccttttgctg atggagctgc acatgaaccc attcaaaggc
       61 cggcattttc agcgtgacat cattctgtgg gccgtacgct ggtactgcaa atacggcatc
      121 agttaccgtg agctgcagga gatgctggct gaacgcggag tgaatgtcga tcactccacg
      181 atttaccgct gggttcagcg ttatgcgcct gaaatggaaa aacggctgcg ctggtactgg
      241 cgtaaccctt ccgatctttg cccgtggcac atggatgaaa cctacgtgaa ggtcaatggc
      301 cgctgggcgt atctgtaccg ggccgtcgac agccggggcc gcactgtcga tttttatctc
      361 tcctcccgtc gtaacagcaa agctgcatac cggtttctgg gtaaaatcct caacaa
//
LOCUS       DAHHTG010000383          408 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.391, whole genome shotgun sequence.
ACCESSION   DAHHTG010000383 DAHHTG010000000
VERSION     DAHHTG010000383.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 408)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 408)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..408
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.391"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            complement(<1..>408)
                     /locus_tag="NBI74_004774"
     CDS             complement(<1..>408)
                     /locus_tag="NBI74_004774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCD1934965.1"
                     /translation="VNFDKPDLSLLKVADYPSIILLAMTLGCLEYTLEEGARWGWLDD
                     NTIFLTSVLALVSFILFAARTLTISNPIMDLHAFKDKNFTLGCFFSFSGGVGIFSTVY
                     LIPVFLGRVRGLNAEEIGFAVCTTGIFQLFSVP"
BASE COUNT          155 a           92 c           68 g           93 t
ORIGIN      
        1 agggaacaga aaataactgg aaaatacctg ttgtgcaaac ggcaaaacct atctcttcgg
       61 catttaatcc gcgaacccgc cccaaaaaca caggtattaa atatacagta gagaatattc
      121 caacccctcc agaaaaagaa aaaaaacatc ccagtgtgaa atttttatcc ttaaaagcat
      181 gaagatccat tatcggattg gatattgtaa gtgtacgcgc agcaaataaa ataaaactca
      241 ccaatgccag aactgatgtc agaaaaatag tattatcatc cagccatccc cagcgcgctc
      301 cttcttcaag ggtatattcc agacatccca gtgtcatggc caacaggata atactcggat
      361 aatcagcaac tttcagtaaa gagagatcag gtttatcaaa attaacca
//
LOCUS       DAHHTG010000384          405 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.392, whole genome shotgun sequence.
ACCESSION   DAHHTG010000384 DAHHTG010000000
VERSION     DAHHTG010000384.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 405)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 405)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..405
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.392"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            complement(<1..>405)
                     /locus_tag="NBI74_004775"
     CDS             complement(<1..>405)
                     /locus_tag="NBI74_004775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001152330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1934966.1"
                     /translation="VYKDYSPWKFKKWIHYGDNASSDGVKAKELGINSHVHAIPKLNA
                     YETGLINKIRNYDAYLLSGLFARMRIDSVNEKDYFCYAHVSGYMIPYVAWCVRNALEK
                     GINTLYFISRDGHFLKEIADAYINMHQLSIKT"
BASE COUNT          137 a           79 c           52 g          137 t
ORIGIN      
        1 ttgtttttat acttaattga tgcatattaa tatatgcatc agcgatttcc ttaagaaaat
       61 gaccatcacg tgaaataaaa tataaagtat ttataccttt ctctaatgca tttctcacac
      121 accaagcgac atagggaatc atataccccg acacatgcgc ataacaaaaa tagtcttttt
      181 cattaacact atcaatacgc attcgagcaa ataatccgct aagaagatac gcatcatagt
      241 tacgtatttt gtttattaaa cctgtttcat atgcatttag cttagggatt gcatgtacat
      301 gggaattaat ccctaactct ttggctttaa cgccatcaga acttgcatta tcaccataat
      361 gaatccactt tttaaatttc catggagaat aatctttata gactt
//
LOCUS       DAHHTG010000385          396 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.394, whole genome shotgun sequence.
ACCESSION   DAHHTG010000385 DAHHTG010000000
VERSION     DAHHTG010000385.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 396)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 396)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..396
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.394"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            <1..213
                     /locus_tag="NBI74_004776"
     CDS             <1..213
                     /locus_tag="NBI74_004776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ZinT/AdcA family metal-binding protein"
                     /protein_id="HCD1934967.1"
                     /translation="YLFECKDPESKAPKYIQFSDHIIAPRKSSHFHIFMGNDSQQSLL
                     NEMENWPTYYPYQLSSEEVVEEMMSH"
BASE COUNT          142 a           73 c           67 g          114 t
ORIGIN      
        1 tacttattcg aatgtaaaga tcctgaaagc aaagccccta aatatataca atttagcgat
       61 catataattg caccacgaaa atcatctcat tttcacattt ttatgggtaa tgattcccag
      121 caatctttat tgaatgaaat ggaaaactgg ccaacgtatt atccctatca gttgagtagc
      181 gaagaagtgg tcgaggagat gatgtctcat tgattttttt gcgctgactt cagttattta
      241 acagaacagt gcctggtagt aacaacaata atttaccaag acaaataagt aagacaaact
      301 cagacacgct catacttgag tcgtgtcttt tttattatgt ctgtagaaaa aaactgcgaa
      361 aaaggcgaac aataaataca caactcagcc atacag
//
LOCUS       DAHHTG010000386          389 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.395, whole genome shotgun sequence.
ACCESSION   DAHHTG010000386 DAHHTG010000000
VERSION     DAHHTG010000386.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 389)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 389)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..389
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.395"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            complement(<1..>389)
                     /gene="wzy"
                     /locus_tag="NBI74_004777"
     CDS             complement(<1..>389)
                     /gene="wzy"
                     /locus_tag="NBI74_004777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="O16 family O-antigen polymerase"
                     /protein_id="HCD1934968.1"
                     /translation="SYAFIVGVNRVKHYVYLITAVGVLFSLYMLFLRGLPGGMAYYLS
                     MYLVSPIIAFQEFYFQQVSNSASSHVFWFFERLMGLLTGGVSMSLHKEFVWVGLPTNV
                     YTAFSDYVYISAELSYLMMVIHGCISG"
BASE COUNT          156 a           89 c           61 g           83 t
ORIGIN      
        1 cacctgaaat acagccatga ataaccatca tcaaatagct tagctccgcg gaaatataaa
       61 cataatccga aaaagcagta taaacatttg ttggcaaacc cacccacaca aattctttat
      121 gcaacgacat agagactcca cctgttaata gccccatcag cctttcaaaa aaccaaaaga
      181 catgagaact ggcagagtta gatacttgct gaaaataaaa ctcctgaaac gcgattatag
      241 ggctgaccaa atacatggat agataatatg ccatcccccc aggcagtcca cgtaaaaaga
      301 gcatatacaa ggagaataga acacctacag ctgtaataag ataaacataa tgttttactc
      361 tattaacacc tacgatgaat gcataagag
//
LOCUS       DAHHTG010000387          383 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.396, whole genome shotgun sequence.
ACCESSION   DAHHTG010000387 DAHHTG010000000
VERSION     DAHHTG010000387.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 383)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 383)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..383
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.396"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
BASE COUNT          143 a           92 c           35 g          113 t
ORIGIN      
        1 ctatatacaa ggcacatctc cagaatataa aagcacagac aaacaaccta aaaaaacaac
       61 cccatacata taaattattt tataggtaaa atagattata tattctcata gctaccacaa
      121 actatactag cctgaactat atttattctg ctgcaatcaa tgcatacata acacaaatat
      181 cactcaggta cactactcaa accacgctgg gatttttcct caagttataa tcatcacccc
      241 gaaaatcatt cggcatttac tcattaaata gtcaccccat aggcctgtac atgtttactc
      301 agaaatatac atccttttct ctgtcataaa ccctctgatt aatcataaat aaatacttgt
      361 gacaccaatc tttttcctta acg
//
LOCUS       DAHHTG010000388          369 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.397, whole genome shotgun sequence.
ACCESSION   DAHHTG010000388 DAHHTG010000000
VERSION     DAHHTG010000388.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 369)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 369)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..369
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.397"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
BASE COUNT          126 a           74 c           58 g          111 t
ORIGIN      
        1 catgtacgat gctcccgtta ttttaatttc gttctagctg tacttttgaa tacaaccata
       61 actgattatt gccaactgac tgaatgaaaa atatcaataa atacaacaaa cttactgcct
      121 tttcgtttta tttataagac gttccaacta caagaacaat ataggcacat gattattcca
      181 acaatactgg acaaaaactc ccgtatctga agatacaata ggtgataatt ctttcagaga
      241 ttttgctcaa ctatggcctg atgggagagt catgacggaa ctaaggcctt tacaagccag
      301 aagattagtc ttaatgcgat cacagttcaa cttgctacga ctgaaatccg taatagcata
      361 taaactctg
//
LOCUS       DAHHTG010000389          355 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.398, whole genome shotgun sequence.
ACCESSION   DAHHTG010000389 DAHHTG010000000
VERSION     DAHHTG010000389.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..355
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.398"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            complement(120..>355)
                     /locus_tag="NBI74_004778"
     CDS             complement(120..>355)
                     /locus_tag="NBI74_004778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1934969.1"
                     /translation="INLNESRLRSSDPAAPAIQAINAFMDKGNHLQDLYSNKSLSKAD
                     LERRNEILRSQVISGLQASEELAKLLIASGVML"
BASE COUNT          108 a           72 c           65 g          110 t
ORIGIN      
        1 aaaaccagaa taaaatattt gtgacatata tcgcaggttg ttcggtttcc taaggcatag
       61 tacagcatat tgagcaatgc cagtattcgt cgattttgca catcgttcat aatgaccccc
      121 tataacataa ctccgctggc aataagaagt tttgccagct cctctgacgc ctgtagacca
      181 ctaataactt gagaacgaag gatctcattt ctacgctcta aatccgcttt tgaaagagat
      241 ttattagagt ataaatcttg tagatgattt cctttatcca tgaaggcatt aatagcttgt
      301 attgctggag ccgccggatc agatgaacgc aatctgcttt cattaagatt aatta
//
LOCUS       DAHHTG010000390          355 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.399, whole genome shotgun sequence.
ACCESSION   DAHHTG010000390 DAHHTG010000000
VERSION     DAHHTG010000390.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..355
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.399"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
BASE COUNT           68 a          106 c           84 g           97 t
ORIGIN      
        1 gtgtttcgca tacatgtcaa aacaccttcg accggcagtg cttacgtatc actgccagtt
       61 cctcctgcac ggcctgtcag atcacgtagg ccgtggtgac gttttcaacc cacagaggcg
      121 gattaacggg ttcatgttct tcagcctttc agtacttaac cttgaggatc atctcggctt
      181 agtgatctcg cctcaatccc cgttgtcagc gggttacatc accctgcggg catgccgcag
      241 gtcactgccg ctcaggttct ccaccgtcac acccggtggg attgttgggt ttctcatcgt
      301 gagttaccgg ttcaatattc cagacagact cgcggttcat ttaagcatcc atgcc
//
LOCUS       DAHHTG010000391          354 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.400, whole genome shotgun sequence.
ACCESSION   DAHHTG010000391 DAHHTG010000000
VERSION     DAHHTG010000391.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 354)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 354)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..354
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.400"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            48..124
                     /locus_tag="NBI74_004779"
     tRNA            48..124
                     /locus_tag="NBI74_004779"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:82..84,aa:Ile,seq:gat)
     gene            167..242
                     /locus_tag="NBI74_004780"
     tRNA            167..242
                     /locus_tag="NBI74_004780"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:200..202,aa:Ala,seq:tgc)
BASE COUNT          101 a           77 c           86 g           90 t
ORIGIN      
        1 agtgctcaca cagattgtct gatgaaaatg agcagtaaaa cctctacagg cttgtagctc
       61 aggtggttag agcgcacccc tgataagggt gaggtcggtg gttcaagtcc actcaggcct
      121 accaaatttg caccgcaaat ttgaagaggt tttaactaca tgttatgggg ctatagctca
      181 gctgggagag cgcctgcttt gcacgcagga ggtctgcggt tcgatcccgc atagctccac
      241 catctctgta gtgattaaat aaaaatactt cagagtgtac ctgcaaaggt tcactgcgaa
      301 gttttgctct ttaaaaatct ggatcaagct gaaaattgaa acactgaaca acga
//
LOCUS       DAHHTG010000392          345 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.401, whole genome shotgun sequence.
ACCESSION   DAHHTG010000392 DAHHTG010000000
VERSION     DAHHTG010000392.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 345)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 345)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..345
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.401"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            complement(<1..163)
                     /locus_tag="NBI74_004781"
     CDS             complement(<1..163)
                     /locus_tag="NBI74_004781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000012020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate epimerase"
                     /protein_id="HCD1934970.1"
                     /translation="MSGAYASVIDGNIFLAGGAYFPGAAEKYSNGEYWSHKGLDKAYS
                     KEIYQLIKND"
BASE COUNT           90 a           94 c           65 g           96 t
ORIGIN      
        1 agtcattttt tattagctga tatatctctt ttgaataggc tttatccagt cctttatgag
       61 accagtattc accattgcta tacttttctg ccgcccccgg gaaatatgca ccaccggcca
      121 gaaagatatt tccatcaata acagaggcat atgctcctga caccccctcg ggtgaagcta
      181 ctggtggaag ttcagaccat ttaattcgat catgcgaaag ctcccccgtc caggatctaa
      241 cagaacgaag ccccggtttt ctctcccctc ccagtatata aatttgctgt tctgcaaaaa
      301 tcaccgatga accagcagtt cccggaaatg gagtcgttcc caggt
//
LOCUS       DAHHTG010000393          340 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.402, whole genome shotgun sequence.
ACCESSION   DAHHTG010000393 DAHHTG010000000
VERSION     DAHHTG010000393.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 340)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 340)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..340
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.402"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            complement(<1..242)
                     /locus_tag="NBI74_004782"
     CDS             complement(<1..242)
                     /locus_tag="NBI74_004782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HCD1934971.1"
                     /translation="MDKKQVTDLRSELLDSRFGAKSISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNARQNLATFCQTWDDENVHKLMDLSI"
BASE COUNT          108 a           66 c           67 g           99 t
ORIGIN      
        1 ttaatggata aatccatcaa cttatggaca ttttcgtcgt cccaggtctg gcagaaagtg
       61 gccaggttct gacgagcgtt gccatcaaga tataattcgt cattgataat ctggaatgcg
      121 acatcatcac gcatttcgtg cagcggaaaa cgttttgatt ctgcgatagt ggaaatagac
      181 ttcgcaccaa aacgtgaatc gagtagttcc gaccttaaat ccgttacttg cttcttatcc
      241 attttaaact ccttaaaatg attggatcgc attaaaaaag taggatttat cgataaagta
      301 agcaagtgca aaggactcgg ttttaaataa caaaatccta
//
LOCUS       DAHHTG010000394          339 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.403, whole genome shotgun sequence.
ACCESSION   DAHHTG010000394 DAHHTG010000000
VERSION     DAHHTG010000394.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 339)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 339)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..339
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.403"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            <1..>339
                     /locus_tag="NBI74_004783"
     CDS             <1..>339
                     /locus_tag="NBI74_004783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001034089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1934972.1"
                     /translation="AKLILDGSVNTKNDVEVSNASLTMQGHATEHAIFRSTANHCSLV
                     FLCGTDWVTVLKETESSYNKKFNSDHKSNNQQTSFDQPDWKTGVFKFDTLHLNNADFS
                     ISRNANVEGNI"
BASE COUNT          120 a           63 c           67 g           89 t
ORIGIN      
        1 gcaaaattaa ttctggatgg tagtgtcaac acaaaaaatg atgttgaagt cagtaatgcc
       61 agtcttacca tgcaaggcca tgcaacagag catgcaatat tcagaagcac agcgaatcat
      121 tgctccctgg tatttctttg tggaacggac tgggtcaccg ttttgaaaga aacagagagt
      181 tcatataata aaaagttcaa ttctgatcac aaaagtaata atcagcagac ctcatttgat
      241 cagccagact ggaaaaccgg ggtgtttaaa tttgatacat tacacctgaa caatgctgac
      301 ttttcaatat cacgcaatgc caatgttgaa ggaaatata
//
LOCUS       DAHHTG010000395          333 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.404, whole genome shotgun sequence.
ACCESSION   DAHHTG010000395 DAHHTG010000000
VERSION     DAHHTG010000395.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 333)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 333)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..333
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.404"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            110..259
                     /locus_tag="NBI74_004784"
     CDS             110..259
                     /locus_tag="NBI74_004784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000850858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin activation protein"
                     /protein_id="HCD1934973.1"
                     /translation="MEKVISHTRYKLTPAELEAFNSAVDHRLAELTMNKLYDRVPASV
                     WKYVT"
BASE COUNT           91 a           79 c           85 g           78 t
ORIGIN      
        1 agtctggtgc ggtatgctgg cagtatcacg ttgattacag cataaagtaa cgagagacga
       61 atggaagtta aaaccagagc agttccgtcg ttgttcatcc cgggagacgc tggaaaaagt
      121 gatttcccac acacgttata aacttacccc tgcggagctg gaagccttta actctgcggt
      181 cgatcaccgg ctggcagaac tgacaatgaa caaactttac gatcgcgtgc cggcttccgt
      241 ctggaaatat gtcacctgaa tcttgaatcc gcgctacgat gactccggtg ttatctgtgc
      301 accggaagat cgggaacatt aagcagtata acg
//
LOCUS       DAHHTG010000396          326 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.405, whole genome shotgun sequence.
ACCESSION   DAHHTG010000396 DAHHTG010000000
VERSION     DAHHTG010000396.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 326)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 326)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..326
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.405"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            <1..>326
                     /locus_tag="NBI74_004785"
     CDS             <1..>326
                     /locus_tag="NBI74_004785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HCD1934974.1"
                     /translation="GHEDYIKKLLENLNADDEHYKIIVRDNKDSLLLKQICQHYAGLD
                     YISGGVYGFGHNNNIAVAYVKEKYRPADDDYILFLNPDIIMKHDDLLTYIKYVESKRY
                     AFSTLC"
BASE COUNT          113 a           52 c           64 g           97 t
ORIGIN      
        1 acggacatga agactacatc aaaaaattac tcgaaaatct taatgctgac gatgagcact
       61 acaagattat cgtacgcgac aacaaagact ctctattatt gaaacaaata tgccagcatt
      121 atgcaggcct ggactatatt agtggaggtg tatacggctt tggtcataat aataatattg
      181 cggtggcgta tgtaaaggaa aaatatagac ccgcagatga tgattacatt ttgtttttga
      241 atcccgatat catcatgaag catgatgatt tgctgacata tattaaatat gtcgaaagta
      301 agcgttatgc ttttagtaca ttatgc
//
LOCUS       DAHHTG010000397          326 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.406, whole genome shotgun sequence.
ACCESSION   DAHHTG010000397 DAHHTG010000000
VERSION     DAHHTG010000397.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 326)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 326)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..326
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.406"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
BASE COUNT          101 a           48 c           67 g          110 t
ORIGIN      
        1 atgcgaaact ttttaccggg aagtattcat ctttgcgtta tttccgagtt tacagagtgg
       61 atatcccatg aaattatgtt gttacaaagc atgggggtga ctgtatgaat agtcaaaatg
      121 ctttaagtaa tatgaggctt gctgtgagag gggatatatc cggtaactct acattggttc
      181 ttaaaaagat tagattctac aattgtgcca ttatttatct tcgcaatgcc caattacttg
      241 ttaaaacgat agatgggggg gttattaata tcccacctga atcaatatgt tatattgaga
      301 aaaatacagt cattgatgta acactt
//
LOCUS       DAHHTG010000398          324 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.407, whole genome shotgun sequence.
ACCESSION   DAHHTG010000398 DAHHTG010000000
VERSION     DAHHTG010000398.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 324)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 324)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..324
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.407"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
BASE COUNT          111 a           55 c           47 g          111 t
ORIGIN      
        1 ataccaagat gcttttaata ttcatcacat caaagaaaat atcaagtcga tccagacgac
       61 tgaaatatcc ccagagaact atcaacgcaa tccctgatat aatacttgat ggtgttaata
      121 ctgaaactat tagcgtcttc cacttctgat tcattttaac ttcctggttt tgctataaag
      181 agagcataaa aaagagtgaa taagttatta cacttcattg tttgactcct cgcagtaacc
      241 tttcaggaag gttgtcggat atttttgata aattatgtca taaacatatt attgtaagta
      301 atgatttcac taaatttatt tgat
//
LOCUS       DAHHTG010000399          321 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.408, whole genome shotgun sequence.
ACCESSION   DAHHTG010000399 DAHHTG010000000
VERSION     DAHHTG010000399.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..321
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.408"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            <1..>321
                     /locus_tag="NBI74_004786"
     CDS             <1..>321
                     /locus_tag="NBI74_004786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="inovirus-type Gp2 protein"
                     /protein_id="HCD1934975.1"
                     /translation="DAYQKRKHREGKRVHPTTLHYVWAREFGECKGKKHYHLMLLVNR
                     DTWCRAGDYRAPGSLAGMIKQAWCSALGVDAGRYDTLAHFPVRPAVWLERDDDTGFQQ
                     VLERA"
BASE COUNT           68 a           83 c          107 g           63 t
ORIGIN      
        1 cgacgcttac cagaaacgta agcatcggga aggtaaacgc gtgcatccca caaccctgca
       61 ttacgtctgg gcccgggagt ttggggagtg caaaggtaaa aaacactatc acctgatgct
      121 gctggtcaac cgggatacct ggtgtcgtgc cggcgattac cgcgctccgg gatcactggc
      181 cgggatgatt aaacaggcgt ggtgcagtgc cctgggagtg gatgccggac gttatgacac
      241 gctggcacat tttccggtca ggccggcggt gtggctggaa cgcgatgatg acaccggctt
      301 tcagcaggtg ctggagcgtg c
//
LOCUS       DAHHTG010000400          307 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.409, whole genome shotgun sequence.
ACCESSION   DAHHTG010000400 DAHHTG010000000
VERSION     DAHHTG010000400.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 307)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 307)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..307
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.409"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            complement(82..234)
                     /gene="hokA"
                     /locus_tag="NBI74_004787"
     CDS             complement(82..234)
                     /gene="hokA"
                     /locus_tag="NBI74_004787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001135724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokA"
                     /protein_id="HCD1934976.1"
                     /translation="MPQKYRLLSLIVICFTLLFFTWMIRDSLCELHIKQENYELAAFL
                     ACKLKE"
BASE COUNT           95 a           62 c           63 g           87 t
ORIGIN      
        1 tttttgtgaa gaataaattt gggtgtaatg agaatgcgta acgccgtaag aaaggcggga
       61 ataacttccc gccgaagatt cttactcttt caatttgcag gctaaaaacg cggccagctc
      121 ataattctcc tgtttaatat gcaattcaca cagtgaatct cttatcatcc aggtgaaaaa
      181 taaaagcgtg aaacaaatca ctattaaaga aagtaatcta tatttctgcg gcatcccagc
      241 ctcctgtgtt gatttccaac gagtaagagg ctaaccttat ggtgttcagg catagggtgg
      301 cctcact
//
LOCUS       DAHHTG010000401          306 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.410, whole genome shotgun sequence.
ACCESSION   DAHHTG010000401 DAHHTG010000000
VERSION     DAHHTG010000401.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 306)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 306)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..306
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.410"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            <1..>306
                     /locus_tag="NBI74_004788"
     CDS             <1..>306
                     /locus_tag="NBI74_004788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016158917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HCD1934977.1"
                     /translation="FNENKSWLLRSWISNYSDDGKSKSFIITPILYRVLPNESIQLKI
                     EKTDDLLPTDRESVFRINVLAIPPKEISNDKTSSKPSDLQFAINSRIKLIYRPHKLN"
BASE COUNT          118 a           51 c           44 g           93 t
ORIGIN      
        1 actttaatga aaataaatct tggctattac gttcatggat atccaactat agcgatgatg
       61 gaaaaagtaa aagtttcatt ataactccaa ttctttacag agtattgcct aatgaaagta
      121 ttcaattaaa aatcgaaaaa acagatgact tgttaccaac agatagagaa tctgttttcc
      181 gaattaatgt gcttgcaatt ccaccaaaag agattagtaa tgataaaacc agttcgaaac
      241 cttctgattt acaatttgca attaatagca gaatcaaact catttatcgg cctcataagc
      301 ttaacg
//
LOCUS       DAHHTG010000402          304 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.411, whole genome shotgun sequence.
ACCESSION   DAHHTG010000402 DAHHTG010000000
VERSION     DAHHTG010000402.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 304)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 304)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..304
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.411"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
BASE COUNT           98 a           67 c           57 g           82 t
ORIGIN      
        1 tactgtaagc atcttacagc caatacagaa tttagttatt tactcacacc tgaaaattat
       61 aatcgccttt tcccaccgac gctcttacct gtcgaggaaa aaccagaaat accgcctgaa
      121 cgagaaatta tcctcaccat tggcgtattc tttgatggca caggaaacaa tttaaccaat
      181 acgaatttac gtatgagttt ttgccaacca gaaacctatg gtctggatgt ccaggatttg
      241 gcctcgttta ataaacaatg tatgagcaag cagggtaaaa cgggatccgg agtacagagc
      301 tatt
//
LOCUS       DAHHTG010000403          302 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.412, whole genome shotgun sequence.
ACCESSION   DAHHTG010000403 DAHHTG010000000
VERSION     DAHHTG010000403.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 302)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 302)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..302
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.412"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            complement(<1..>302)
                     /locus_tag="NBI74_004789"
     CDS             complement(<1..>302)
                     /locus_tag="NBI74_004789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCD1934978.1"
                     /translation="NNHLDIGVGTGFYLTHVPESSLISLMDLNEASLNAASTRAGESK
                     IKHKISHDVFDPYPAALHGQFDSISMFYLLHCLPGNISTKSCVIRNAAQALTDDGTL"
BASE COUNT           89 a           64 c           65 g           84 t
ORIGIN      
        1 agagttccat cgtcagttaa ggcctgcgcc gcattgcgta ttacacagct ttttgtagat
       61 atatttccag gcaggcagtg aagaaggtaa aacatggaaa tggaatcaaa ttgaccatgt
      121 aacgccgcgg gataaggatc aaaaacatca tggctaattt tatgtttaat ttttgattcc
      181 ccagcccttg tcgatgccgc gttcaggcta gcttcgttca aatccattaa agatatcaga
      241 ctactctcag gtacgtgagt aaggtaaaac ccagttccaa cgccaatatc cagatggttg
      301 tt
//
LOCUS       DAHHTG010000404          292 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.413, whole genome shotgun sequence.
ACCESSION   DAHHTG010000404 DAHHTG010000000
VERSION     DAHHTG010000404.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 292)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 292)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..292
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.413"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
BASE COUNT          101 a           63 c           42 g           86 t
ORIGIN      
        1 cgaataattt agcctcagct ggataataat ttatgcaatc aatcctccct gaattaaaaa
       61 acttttcacg taatatgtac gattgttcat ctgcgaaaaa agatgcaggc ataactatag
      121 ctaccaatcc atttttatta catatttcta agctaagttc tgcaccaaca cgtgaaagat
      181 tagtccccca cccagcaaat ttacgcttcg gctgagaata tggcaatact cttgacaaat
      241 aatcatcaaa ctcacgaaga gagtctatat acatcgattt catagatggt tc
//
LOCUS       DAHHTG010000405          291 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.414, whole genome shotgun sequence.
ACCESSION   DAHHTG010000405 DAHHTG010000000
VERSION     DAHHTG010000405.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 291)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 291)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..291
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.414"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            complement(148..>291)
                     /gene="tuf"
                     /locus_tag="NBI74_004790"
     CDS             complement(148..>291)
                     /gene="tuf"
                     /locus_tag="NBI74_004790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HCD1934979.1"
                     /translation="VEMVMPGDNIKMVVTLIHPIAMDDGLRFAIREGGRTVGAGVVAK
                     VLG"
BASE COUNT           93 a           67 c           56 g           75 t
ORIGIN      
        1 aacattcata tttaaaatat taaattgaat tgatatttta aatatgaata agttattcgt
       61 tctgacagta cgaataagat atgcagtcaa caaatgcaaa aagggcgccg aagcgccctt
      121 ttcaattcaa aactaattaa cgtgtaatta gcccagaact ttagcaacaa cgcccgcgcc
      181 aacggtacgg ccgccttcac ggattgcgaa acgcagaccg tcgtccatcg cgatcgggtg
      241 gatcagggta acaaccattt tgatgttgtc gcccggcatt accatctcta c
//
LOCUS       DAHHTG010000406          289 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.415, whole genome shotgun sequence.
ACCESSION   DAHHTG010000406 DAHHTG010000000
VERSION     DAHHTG010000406.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 289)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 289)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..289
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.415"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            <1..>289
                     /locus_tag="NBI74_004791"
     CDS             <1..>289
                     /locus_tag="NBI74_004791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000197723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2931 family protein"
                     /protein_id="HCD1934980.1"
                     /translation="WYFLFFYPNALPAQVTYVELLDTDGIYYQFRTLDATAPSSTTIG
                     QWRDNLRVGVLSFNKAKNPPMSIHFCWDSVIDKKVYETWITLAGEVWKLMLT"
BASE COUNT           78 a           60 c           63 g           88 t
ORIGIN      
        1 tggtatttcc tttttttcta ccctaatgcg ctaccagcac aagttaccta tgtagaacta
       61 ttggacactg atggtatcta ttatcagttc agaacgctgg atgctaccgc tccgagttcc
      121 actactattg gtcagtggag agataatctt cgggttgggg tgttatcatt caataaagcc
      181 aaaaatcccc ctatgtctat ccatttctgc tgggattcag tgattgataa aaaagtgtat
      241 gaaacctgga taacgctggc gggagaagtc tggaaattga tgctcacac
//
LOCUS       DAHHTG010000407          286 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.416, whole genome shotgun sequence.
ACCESSION   DAHHTG010000407 DAHHTG010000000
VERSION     DAHHTG010000407.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 286)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 286)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..286
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.416"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            <1..>286
                     /locus_tag="NBI74_004792"
     CDS             <1..>286
                     /locus_tag="NBI74_004792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001546116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCD1934981.1"
                     /translation="KQYLKKSSGMLLNFTSSSYTRGRSYYALYSSSKAAIVNFTQAIA
                     EEWSSENIKVNCINPERTATPMRTINFGIEPAELLLDPRDVALTSLKVLGA"
BASE COUNT           91 a           60 c           49 g           86 t
ORIGIN      
        1 aagcaatatt tgaagaaatc ttcagggatg ctacttaatt ttacttcaag ctcatacact
       61 cgtggccgtt catattatgc tctttattcc tcatcaaaag ctgctattgt caactttaca
      121 caagcaatag ctgaggaatg gtcatctgaa aatattaaag taaactgtat taaccctgaa
      181 cgaactgcaa ctccaatgcg taccatcaac tttggcattg aacctgcaga gttattatta
      241 gatcctagag atgttgccct gacaagcctc aaagtattag gagctg
//
LOCUS       DAHHTG010000408          286 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.417, whole genome shotgun sequence.
ACCESSION   DAHHTG010000408 DAHHTG010000000
VERSION     DAHHTG010000408.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 286)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 286)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..286
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.417"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            complement(<1..233)
                     /locus_tag="NBI74_004793"
     CDS             complement(<1..233)
                     /locus_tag="NBI74_004793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000471929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HCD1934982.1"
                     /translation="MFKEKLEDYTEEEFLNFLGGLRSSMKDGKPLKGKELEMYWDSLV
                     DHFIEITQHPSGSDLIFYPKSQGDDKPENILKIV"
BASE COUNT           80 a           59 c           43 g          104 t
ORIGIN      
        1 acaattttca ggatgttttc aggcttatca tctccttgac ttttagggta gaaaattaga
       61 tcgctccctg acggatgctg tgtaatttca ataaaatgat ctactaatga atcccagtac
      121 atttctagct ccttaccttt taatggcttt ccatctttca tagatgatct caatccacct
      181 aaaaaattaa gaaattcctc ctcggtataa tcttcgagct tttctttaaa catatttatc
      241 tcctgtagtg aatctcatcg tgtagtctgg gggtaacaat tcgtaa
//
LOCUS       DAHHTG010000409          282 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.418, whole genome shotgun sequence.
ACCESSION   DAHHTG010000409 DAHHTG010000000
VERSION     DAHHTG010000409.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 282)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 282)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..282
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.418"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            <1..>282
                     /locus_tag="NBI74_004794"
     CDS             <1..>282
                     /locus_tag="NBI74_004794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001606949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2931 family protein"
                     /protein_id="HCD1934983.1"
                     /translation="PNSLPAQVTYVELLDTDGIYYQFRTLDSTIPVSTTIGQWQENLS
                     GGMTAFNKAKNPPMSIHFCWDSVIDKKEYETWITLAGDVWKLMLTPYSPP"
BASE COUNT           89 a           65 c           55 g           73 t
ORIGIN      
        1 ccctaattcc ctaccagcac aagttaccta tgtggaatta ttagataccg atggcatcta
       61 ttatcagttc agaactctgg atagcactat ccctgtctca acaacaatag gacaatggca
      121 ggaaaatctt agtggtggaa tgacggcatt caataaagcc aaaaatcccc ctatgtctat
      181 ccatttctgc tgggattcag tgattgataa aaaagagtat gaaacctgga taacgctggc
      241 gggagacgtc tggaaattga tgctcacacc ttattcacca cc
//
LOCUS       DAHHTG010000410          282 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.419, whole genome shotgun sequence.
ACCESSION   DAHHTG010000410 DAHHTG010000000
VERSION     DAHHTG010000410.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 282)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 282)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..282
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.419"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
BASE COUNT          104 a           68 c           39 g           71 t
ORIGIN      
        1 gcggctctct ctgctcgcta attccgcaag cctgacaaaa ccaaagtgaa taaaaatgca
       61 attcaaccga ataaaagaaa acatctatca ctggtgcttt ctgctgtcaa tgtacattat
      121 ctgtcataaa aaatgctgaa aatgacccgg tcactacata caccgaattc tctacgctta
      181 tagcgttacc tcacccccaa accttcatga gtgaaaataa aggcaagcac atatccaatt
      241 gatttaatta aaaataaatc atatcggcat gtaaaaatcc ac
//
LOCUS       DAHHTG010000411          278 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.420, whole genome shotgun sequence.
ACCESSION   DAHHTG010000411 DAHHTG010000000
VERSION     DAHHTG010000411.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 278)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 278)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..278
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.420"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
BASE COUNT          112 a           37 c           57 g           72 t
ORIGIN      
        1 attcataatt atgaattctg aatgtaaaca ttcacgaata ttatggatgt atatcaatat
       61 gttatgagtt gatcgaaaag atagggagta tcaatgattg ttgcgctaaa cggctggtgt
      121 gatctcgcgc acattaatga aggcataaaa aagagccggc atcatgccgg cccaaaaaat
      181 ataaacaaga cgataaaagg ctaataacgg aggcatgatg acacagaaaa ataaaaaggt
      241 ataattgtaa tcagtaccaa tatattgatt gaatgaat
//
LOCUS       DAHHTG010000412          278 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.denovo.421, whole genome shotgun sequence.
ACCESSION   DAHHTG010000412 DAHHTG010000000
VERSION     DAHHTG010000412.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 278)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 278)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..278
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.denovo.421"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            <1..>278
                     /locus_tag="NBI74_004795"
     CDS             <1..>278
                     /locus_tag="NBI74_004795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HCD1934984.1"
                     /translation="SSTMKTMNGWILNSEHYAIPQRRKRVILVGSNDPLFSIEPPQKL
                     TEDKESWVSVKDALSDLPPLQHGEDGSGKYYIHHPENDYQLFMRGNIT"
BASE COUNT           96 a           51 c           49 g           82 t
ORIGIN      
        1 tctcatcaac catgaaaact atgaatggat ggatattaaa ctctgaacat tatgcaatcc
       61 ctcagcgccg aaaaagggtt atattggttg gttcaaacga ccctttattt tcaattgaac
      121 ctccacaaaa attaacagaa gataaggaat cttgggtatc tgtaaaggat gcattatctg
      181 atctgcctcc tttgcaacat ggtgaagatg gaagtggtaa atattatatc catcaccccg
      241 aaaatgacta tcaattattc atgagaggta atattact
//
LOCUS       DAHHTG010000413        15642 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.guided.100, whole genome shotgun sequence.
ACCESSION   DAHHTG010000413 DAHHTG010000000
VERSION     DAHHTG010000413.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15642)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15642)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15642
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.guided.100"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            complement(251..796)
                     /gene="orn"
                     /locus_tag="NBI74_004796"
     CDS             complement(251..796)
                     /gene="orn"
                     /locus_tag="NBI74_004796"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HCD1934985.1"
                     /translation="MSANENNLIWIDLEMTGLDPERDRIIEIATLVTDANLNILAEGP
                     TIAVHQSDEQLALMDDWNVRTHTASGLVERVKASTMGDREAELATLEFLKQWVPAGKS
                     PICGNSIGQDRRFLFKYMPELEAYFHYRYLDVSTLKELARRWKPEILDGFTKQGTHQA
                     MDDIRESVAELAYYREHFIKL"
     gene            891..1943
                     /gene="rsgA"
                     /locus_tag="NBI74_004797"
     CDS             891..1943
                     /gene="rsgA"
                     /locus_tag="NBI74_004797"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small ribosomal subunit biogenesis GTPase RsgA"
                     /protein_id="HCD1934986.1"
                     /translation="MSKNKLSKGQQRRVNANHQRRLKTSKEKPDYDDNLFGEPDEGIV
                     ISRFGMHADVESADGDVHRCNIRRTIRSLVTGDRVVWRPGKPAAEGVNVKGIVEAVHE
                     RTSVLTRPDFYDGVKPIAANIDQIVIVSAILPELSLNIIDRYLVACETLQIEPIIVLN
                     KIDLLDDEGMEFVNEQMDIYRNIGYRVLMVSSHTQDGLKPLEEALTGRISIFAGQSGV
                     GKSSLLNALLGLQKEILTNDVSDNSGLGQHTTTAARLYHFPHGGDVIDSPGVREFGLW
                     HLEPEQITQGFVEFHDYLGLCKYRDCKHDTDPGCAIREAVEEGKIAETRFENYHRILE
                     SMAQVKTRKNFSDTDD"
     gene            2040..3008
                     /gene="psd"
                     /locus_tag="NBI74_004798"
     CDS             2040..3008
                     /gene="psd"
                     /locus_tag="NBI74_004798"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313166.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HCD1934987.1"
                     /translation="MLNSFKLSLQYILPKLWLTRLAGWGASKRAGWLTKLVIDLFVKY
                     YKVDMKEAQKPDTASYRTFNEFFVRPLRDEVRPIDTDPNVLVMPADGVISQLGKIEED
                     KILQAKGHNYSLEALLAGNYLMADLFRNGTFVTTYLSPRDYHRVHMPCNGILREMIYV
                     PGDLFSVNHLTAQNVPNLFARNERVICLFDTEFGPMAQILVGATIVGSIETVWAGTIT
                     PPREGIIKRWTWPAGENDGSVALLKGQEMGRFKLGSTVINLFAPGKVDLVEQLESLSV
                     TKIGQPLAVSTETFVTPDAEPAPLPAEEIEAEHDASPLVDDKKDQV"
     gene            3030..6353
                     /gene="mscM"
                     /locus_tag="NBI74_004799"
     CDS             3030..6353
                     /gene="mscM"
                     /locus_tag="NBI74_004799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="miniconductance mechanosensitive channel MscM"
                     /protein_id="HCD1934988.1"
                     /translation="MRLIITFLMAWCLSWGAYAATAPDSKQITQELEQAKAAKPAQPE
                     VVEALQSALNALEERKGSLERIKQYQEVIDNYPKLSATLRAQLNNMRDEPRSVSPGMS
                     NDALNQEILQISSQLLDKSRQAQQEQERAREIADSLNQLPQQQTDARRQLNEIERRLG
                     TLTGNTPLNQAQNFALQSDSARLKALVDELELAQLSANNRQELARLRSELAEKESQQL
                     DAYLQALRNQLNSQRQLEAERALESTEQLAESSADLPKDIVAQFKINRELSAALNQQA
                     QRMDLVASQQRQAASQTLQVRQALNTLREQSQWLGSSNLLGEALRAQVARLPEMPKPQ
                     QLDTEMAQLRVQRLRYEDLLNKQPLLRQIHQADGQPLTAEQNRILEAQLRTQRELLNS
                     LLQGGDTLLLELTKLKVSNGQLEDALKEVNEATHRYLFWTSDVRPMTIAWPLEIAQDL
                     RRLISLDTFSQLGKASVMMLTSKETILPLFGALILVGCSIYSRRYFTRFLERSAAKVG
                     KVTQDHFWLTLRTLFWSILVASPLPVLWMTLGYGLREAWPYPLAVAIGDGVTATVPLL
                     WVVMICATFARPNGLFIAHFGWPRERVSRGMRYYLMSIGLIVPLIMALMMFDNLDDRE
                     FSGSLGRLCFILICGALAVVTLSLKKAGIPLYLNKEGSGDNITNHMLWNMMIGAPLVA
                     ILASAVGYLATAQALLARLETSVAIWFLLLVVYHVIRRWMLIQRRRLAFDRAKHRRAE
                     MLAQRARGEEEAHHHSSPEGAIEVDESEVDLDAISAQSLRLVRSILMLIALLSVIVLW
                     SEIHSAFGFLENISLWDVTSTVQGVESLEPITLGAVLIAILVFIITTQLVRNLPALLE
                     LAILQHLDLTPGTGYAITTITKYLLMLIGGLVGFSMIGIEWSKLQWLVAALGVGLGFG
                     LQEIFANFISGLIILFEKPIRIGDTVTIRDLTGSVTKINTRATTISDWDRKEIIVPNK
                     AFITEQFINWSLSDSVTRVVLTIPAPANANSEEVTEILLTAARRCSLVIDNPAPEVFL
                     VDLQQGIQIFELRIYAAEMGHRMPLRHEIHQLILAGFHAHGIDMPFPPFQMRLESLNG
                     KQTGRTLTSAGKGRQAGSL"
     gene            complement(6384..6698)
                     /locus_tag="NBI74_004800"
     CDS             complement(6384..6698)
                     /locus_tag="NBI74_004800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjeO family protein"
                     /protein_id="HCD1934989.1"
                     /translation="MSARMSVLCCIWFIVAFLWITITSALDKEWMIDGRGINNVCDVL
                     MYLEDDDTRDVGVIMTLPLFFPFLWFALWRKKRGWFMYATALAIFGYWLWQFFLRYQF
                     CL"
     gene            complement(6796..8298)
                     /gene="yjeM"
                     /locus_tag="NBI74_004801"
     CDS             complement(6796..8298)
                     /gene="yjeM"
                     /locus_tag="NBI74_004801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418580.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/gamma-aminobutyrate family transporter
                     YjeM"
                     /protein_id="HCD1934990.1"
                     /translation="MPHSIKKMSLIGLILMIFTSVFGFANSPSAYYLMGYSAISFYIF
                     SALLFFIPFALMMAEMGAAYRKEEGGIYSWMNNSVGPRFAFIGTFMWFSSYIIWMVST
                     SAKVWVPFSTFLYGSDMTQHWHIAGLEPTQVVGLLAVAWMILVTVVASKGINKIARIT
                     AVGGIAVMCLNLVLLLVSITILLLNGGHFAQDINFLASPNPGYQSGLAMLSFVVFAIF
                     AYGGIEAVGGLVDKTENPEKNFAKGIVFAAIVISIGYSLAIFLWGVSTNWQQVLSNGS
                     VNLGNITYVLMKSLGVTLGNALHLSPEASLSLGVWFARITGLSMFLAYTGAFFTLCYS
                     PLKAIIQGTPKALWPEPMTRLNAMGMPSIAMWMQCGLVTVFILLVSFGGGTASAFFNK
                     LTLMANVSMTLPYLFLALAFPFFKARQDLDRPFVIFKTRMSAMIATVVVVLVVTFANV
                     FTIIQPVVEAGDWDSTLWMIGGPVFFSLLAMAIYQNYCSRVVKNPQWVAE"
     gene            complement(8517..9494)
                     /gene="epmA"
                     /locus_tag="NBI74_004802"
     CDS             complement(8517..9494)
                     /gene="epmA"
                     /locus_tag="NBI74_004802"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P--(R)-beta-lysine ligase"
                     /protein_id="HCD1934991.1"
                     /translation="MSETASWQPSASIPNLLKRAAIMAEIRRFFADRGVLEVETPCMS
                     QATVTDIHLVPFETRFVGPGHSQGMNLWLMTSPEYHMKRLLVAGCGPVFQLCRSFRNE
                     EMGRYHNPEFTMLEWYRPHYDMYRLMNEVDDLLQQVLDCPAAESLSYQQAFLRYLEID
                     PLSADKTQLREVAAKLDLSNVADTEEDRDTLLQLLFTFGVEPNIGKEKPTFVYHFPAS
                     QASLAQISTEDHRVAERFEVYYKGIELANGFHELTDAREQQQRFEQDNRKRAARGLPQ
                     HPIDQNLIEALKVGMPDCSGVALGVDRLVMLALGAETLAEVIAFSVDRA"
     gene            9819..11627
                     /gene="frdA"
                     /locus_tag="NBI74_004803"
     CDS             9819..11627
                     /gene="frdA"
                     /locus_tag="NBI74_004803"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HCD1934992.1"
                     /translation="MQTFQADLAIVGAGGAGLRAAIAAAQANPNAKIALISKVYPMRS
                     HTVAAEGGSAAVAQDHDSFEYHFHDTVAGGDWLCEQDVVDYFVHHCPTEMTQLELWGC
                     PWSRRPDGSVNVRRFGGMKIERTWFAADKTGFHMLHTLFQTSLQFPQIQRFDEHFVLD
                     ILVDDGHVRGLVAMNMMEGTLVQIRANAVVMATGGAGRVYRYNTNGGIVTGDGMGMAL
                     SHGVPLRDMEFVQYHPTGLPGSGILMTEGCRGEGGILVNKNGYRYLQDYGMGPETPLG
                     EPKNKYMELGPRDKVSQAFWHEWRKGNTISTPRGDVVYLDLRHLGEKKLHERLPFICE
                     LAKAYVGVDPVKEPIPVRPTAHYTMGGIETDQNCETRIKGLFAVGECSSVGLHGANRL
                     GSNSLAELVVFGRLAGEQATERAATAGNGNEAAIEAQAAGVEQRLKDLVNQDGGENWA
                     KIRDEMGMAMEEGCGIYRTPELMQKTIDKLAELQERFKRVRITDTSSVFNTDLLYTIE
                     LGHGLNVAECMAHSAMARKESRGAHQRLDEGCTERDDVNFLKHTLAFRDADGTTRLEY
                     SDVKITTLPPAKRVYGGEADAADKAEAANKKEKANG"
     gene            11620..12354
                     /gene="frdB"
                     /locus_tag="NBI74_004804"
     CDS             11620..12354
                     /gene="frdB"
                     /locus_tag="NBI74_004804"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur protein"
                     /protein_id="HCD1934993.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALGYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            12365..12760
                     /gene="frdC"
                     /locus_tag="NBI74_004805"
     CDS             12365..12760
                     /gene="frdC"
                     /locus_tag="NBI74_004805"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HCD1934994.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FGLFALKNGPEAWAGFVDFLQNPVIVIINLITLAAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            12771..13130
                     /gene="frdD"
                     /locus_tag="NBI74_004806"
     CDS             12771..13130
                     /gene="frdD"
                     /locus_tag="NBI74_004806"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HCD1934995.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGVVTI"
     gene            13193..14326
                     /locus_tag="NBI74_004807"
     CDS             13193..14326
                     /locus_tag="NBI74_004807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HCD1934996.1"
                     /translation="MFKTTLCTLLITASCSTFAAPQQINDIVHRTITPLIEQQRIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPTTKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMKTRVFQPLKLNHTWINVPSAEEKNYAWG
                     YREGKAVHVSPGALDAEAYGVKSTIEDMARWVQSNLKPLDINEKILQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDIIINGSDNKIALAARPVKPITPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVTAAWQILNTLQ"
     gene            14415..14948
                     /gene="blc"
                     /locus_tag="NBI74_004808"
     CDS             14415..14948
                     /gene="blc"
                     /locus_tag="NBI74_004808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HCD1934997.1"
                     /translation="MRLLPLVAAATAAFLVVACSSPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDREMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(14945..15262)
                     /gene="sugE"
                     /locus_tag="NBI74_004809"
     CDS             complement(14945..15262)
                     /gene="sugE"
                     /locus_tag="NBI74_004809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HCD1934998.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSMA
                     LLAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
     gene            complement(15438..15584)
                     /gene="ecnB"
                     /locus_tag="NBI74_004810"
     CDS             complement(15438..15584)
                     /gene="ecnB"
                     /locus_tag="NBI74_004810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HCD1934999.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
BASE COUNT         3911 a         4148 c         4022 g         3561 t
ORIGIN      
        1 ccttggcaag gtcgtgctct accaactgag ctattcccgc gtactactta attttgcttt
       61 cgtaattttc gcatttctgc gtcgttacgg gaggcgcatt atacgagaaa tctttccctc
      121 tgcaaccccc ctaaaaaatt ttttttgagt ttttatctca agtgctgaaa ttatcagcaa
      181 ggcgttcaat ttagcgtcaa aatcacgccg ccgcaagtgg attccggcgc ttacgtgacc
      241 aggaaaaatt ttacagcttg ataaaatgct cgcggtagta agccaactcc gccaccgatt
      301 cacggatatc atccatcgcc tgatgcgtcc cctgcttggt aaagccgtca agaatttccg
      361 gcttccagcg gcgcgccagc tctttcaggg tgctaacatc gagatagcgg tagtggaagt
      421 aggcttccag ctccggcatg tatttaaaca ggaagcgacg gtcctgaccg atgctgttac
      481 cacaaatcgg cgattttccc gcaggcaccc actgttttaa gaattcgagc gttgccagtt
      541 cagcttcacg atcgcccatc gtgctcgctt tcacacgctc taccagcccg ctggcggtat
      601 gggtgcgcac gttccagtca tccatcagcg ccagctgttc atcggactga tgtactgcaa
      661 tggtcggccc ttctgccaga atattcaggt tggcatcggt caccagcgtg gcaatctcaa
      721 taatgcgatc gcgctcggga tccagaccgg tcatctcaag atcgatccaa atcaggttgt
      781 tttcattggc actcatgcta ttttccaccc attcacagtg actatattga tccacaattg
      841 cgtgtatcat agatgttttg cccatcaggg gcgaccagga gtctgtacga ttgagtaaaa
      901 ataaactctc caaaggccag cagcgccgcg tgaacgccaa tcaccagcgt cgtcttaaaa
      961 cgtctaagga gaagcccgac tacgacgaca atctgtttgg cgagcctgat gaaggtatcg
     1021 tcatcagccg ctttggtatg cacgctgatg tggaatccgc cgatggcgac gttcaccgct
     1081 gcaatattcg ccgtaccatc cgttcgttgg tgaccggcga ccgcgtagtc tggcgtccgg
     1141 gtaaaccggc ggcggaaggc gtaaatgtca aagggatcgt ggaagcggtg catgagcgca
     1201 cctcagtgtt gacgcgcccg gatttctacg acggtgtgaa acctattgct gccaacatcg
     1261 accagattgt cattgtctcc gccattttgc cggagctgtc gctcaatatt atcgaccgtt
     1321 acctggtggc ctgcgaaacc ttgcagattg agccgattat tgtgctcaac aagatagacc
     1381 tgctggacga cgaaggcatg gagttcgtca acgagcagat ggatatctac cgcaatatcg
     1441 gttatcgcgt attgatggtt tccagtcata ctcaggatgg gctgaaaccg ctggaagagg
     1501 cgttgaccgg gcgcatcagc atttttgccg ggcagtctgg cgttggcaaa tccagcctgc
     1561 tgaacgcgtt gctgggtctg caaaaagaga tcctgaccaa cgatgtctct gacaactcgg
     1621 ggcttggtca gcacaccacg accgccgcac gactgtatca cttcccgcac ggcggtgatg
     1681 tgattgattc cccaggtgtg cgtgagtttg gtctctggca cctggagccg gaacaaatca
     1741 ctcagggctt tgtcgaattc catgactatt taggtctgtg taaatatcgc gattgcaaac
     1801 acgataccga tccgggctgc gctatccggg aagcggttga ggaagggaaa atcgcggaaa
     1861 cccgtttcga aaactatcac cgtattctgg aaagcatggc gcaggtaaaa acgcgtaaaa
     1921 acttttctga tacggatgac tgacaattaa gctaaccgtc gttagaatcg tccccttttt
     1981 ttcaggatcc ggcatgtagg ccggatcagg aacgacaaaa caatggcctg gaggctacct
     2041 tgttaaattc atttaaactt tcgctacagt acattctgcc gaaactatgg cttactcgcc
     2101 tggcgggttg gggcgcaagc aagcgggcag gatggctgac aaaactggtt atcgatctgt
     2161 tcgttaaata ctacaaggtc gacatgaaag aggcgcaaaa gccggacacc gccagctacc
     2221 gcacctttaa cgaattcttt gtccgtccgc tgcgtgatga agtacgccca atcgataccg
     2281 atccgaatgt cctggttatg cctgccgatg gcgttatcag ccagttgggt aaaatcgaag
     2341 aagataaaat cctgcaagcc aaaggccaca actacagcct cgaagccctg ctggcaggca
     2401 actatctgat ggcggatctc ttccgtaacg gtacgtttgt gaccacttac ctctccccac
     2461 gtgactacca ccgcgtacac atgccgtgca acggtattct gcgtgaaatg atctacgtgc
     2521 cgggcgatct cttctccgtt aaccatctca cggctcagaa cgtgccgaat ctgtttgccc
     2581 gtaacgaacg cgtgatctgc ctgttcgata ccgaatttgg cccgatggcg cagattctgg
     2641 tcggtgcgac gattgtaggc agcattgaga cggtatgggc gggcaccatt acgccgccgc
     2701 gcgaaggcat catcaagcgc tggacctggc ctgccgggga aaacgacggt tctgtggcac
     2761 tgctgaaagg ccaggaaatg ggtcgcttta aactaggctc taccgttatc aacctgtttg
     2821 caccaggtaa agtggacctg gttgagcaac tggagagcct gtctgtcacg aaaattggcc
     2881 agccgctggc agtatctacc gaaacctttg ttacgccaga tgctgaacca gccccgcttc
     2941 ctgctgaaga gatcgaagca gaacacgacg ccagcccatt ggttgacgat aaaaaagacc
     3001 aggtctaatc catcaaagga aacgctgacg tgcgcctgat tatcactttt ctgatggcct
     3061 ggtgcctcag ttggggggcg tacgccgcga cggcccccga tagcaaacaa atcacccagg
     3121 aactggagca ggcaaaagcg gcgaaacctg cgcagccgga agtcgtagag gcgctccagt
     3181 ctgccttaaa tgcgcttgag gaacgaaaag gttcccttga gcgcatcaag caatatcaag
     3241 aagtcattga taattacccg aaactttccg ccactctgcg cgcgcagtta aacaacatgc
     3301 gtgacgagcc acgcagcgtg tcgccgggaa tgtctaacga tgcgctgaat caggaaattc
     3361 tccagatcag cagtcagttg ctggataaaa gccgtcaggc ccagcaagag caggagcgcg
     3421 cccgcgagat tgccgattcc ctgaatcaac tgccgcaaca gcaaaccgat gcccgccgtc
     3481 agttgaatga aatcgagcgc cgcctgggga cgcttaccgg caataccccg ctcaatcagg
     3541 cacaaaattt cgcgttgcag tctgactccg cacgtctgaa agcgctcgtt gatgaactgg
     3601 agctggcgca gctatctgcc aataaccgcc aggaattagc gcgcttgcgc tcagagctgg
     3661 ccgaaaaaga gagccagcag ctggatgcat atttgcaagc actacgcaac cagttgaata
     3721 gtcaacgcca actggaagcg gaacgggcgc tggaaagtac cgagcaactg gcggaaagta
     3781 gcgccgattt gccaaaagat atcgtcgccc agtttaaaat taaccgcgaa ctatcggcgg
     3841 cactgaatca acaggcgcag cggatggatc tcgttgcctc gcaacagcgt caggctgcca
     3901 gccagacgtt acaggtccgg caggcgttga atacgctgcg tgaacagtcg caatggctgg
     3961 gatcatctaa cctgctcggc gaagcgttgc gggcgcaggt ggcacggcta ccggaaatgc
     4021 caaaaccaca acagcttgat accgaaatgg cgcagttacg tgtgcaacgg ttacgttatg
     4081 aggatctgct taataaacag ccgctgctac ggcaaatcca tcaggccgac ggtcagccgc
     4141 tgaccgctga gcaaaaccgt attctggaag cacagctacg cactcagcgt gagttgctga
     4201 actcattgtt gcagggtggc gacacgctac tgctggagct gaccaagctg aaagtctcca
     4261 acgggcaact ggaggatgca ctgaaagagg tgaacgaagc gacgcaccgc tatctgttct
     4321 ggacctctga cgtgcgcccg atgaccatcg cctggccgct ggaaatcgcc caggatctgc
     4381 gtcgtctcat ttcgctggac accttcagtc agttgggcaa agccagtgtg atgatgctga
     4441 ccagtaaaga gacgattttg ccgctgtttg gcgcgttgat tctggtcggt tgcagtattt
     4501 actcgcgccg ctatttcacc cgttttcttg aacgttcggc ggcgaaagtc ggcaaagtga
     4561 ctcaggatca cttctggctg acgttacgca ctcttttctg gtcgattctc gtcgcgtcac
     4621 cgctgcctgt gctgtggatg acgctgggtt acggcttgcg cgaggcgtgg ccttatccgc
     4681 tggcggtcgc gattggtgat ggcgtaacag ccaccgtgcc gctgctgtgg gtagtgatga
     4741 tttgcgccac ctttgctcgc ccgaacggct tgtttatcgc tcattttggc tggccgcgcg
     4801 aacgtgtttc ccgtgggatg cgttactacc tgatgagcat cgggcttatt gtgccgctga
     4861 ttatggcgct gatgatgttc gataacctcg acgaccgcga attttccggt tcgctgggac
     4921 ggctttgctt tatcctcatt tgcggtgcgc tggcggtggt caccctcagc ctgaaaaagg
     4981 ccgggatccc gctgtatctc aacaaagaag gcagcggcga caacattacc aaccatatgc
     5041 tgtggaacat gatgattggc gcacctctgg ttgccattct ggcgtcggcg gtgggttatc
     5101 tggcaacggc gcaggcgctg ttagcgaggc ttgaaacctc ggttgccatc tggttcctgc
     5161 tactggtggt ttatcacgtt atccgccgct ggatgctgat ccagcgccgc aggctggcgt
     5221 ttgaccgggc aaagcatcgc cgggcagaga tgttagcgca gcgtgcgcgt ggcgaagagg
     5281 aagcgcatca tcacagtagc ccggaaggag caattgaagt cgatgaaagc gaagtcgatc
     5341 tcgatgccat cagtgcgcaa tccttgcggc tggtgcgctc aattttgatg ttgatcgccc
     5401 tgctttcggt cattgtgctg tggtcagaaa tccattccgc tttcggcttc ctcgaaaata
     5461 tttcgctgtg ggatgtcact tccacggtac agggcgtaga aagcctggag ccgattaccc
     5521 tcggtgcggt gctgattgcc attctggtgt ttatcatcac cacgcagctg gtgcgcaacc
     5581 tgcccgcgct gctggaactg gcgattctgc agcacctgga tttaacgccg ggtacgggct
     5641 acgccatcac caccatcacc aaatatctgc tgatgctaat tggcgggctg gtcggcttct
     5701 cgatgattgg tattgagtgg tcgaaattgc agtggctggt cgccgcgctc ggtgttggtc
     5761 tcggttttgg tttgcaggaa attttcgcca actttatctc tggcctgatt atcctgttcg
     5821 aaaaaccgat tcgcattggc gatacggtaa cgatccgcga tcttaccggt agtgtgacga
     5881 aaattaacac ccgcgccacc accatcagcg actgggaccg caaagaaatt atcgtgccga
     5941 acaaggcgtt tattaccgag cagtttatca actggtcgct ctctgactcg gtcacgcgcg
     6001 tggtgttgac gattccggcc cctgccaatg ccaacagtga agaagtgacg gaaatcctgc
     6061 tcaccgcagc gcgtcgctgc tcgctggtga tcgacaaccc ggcgccggaa gtcttcctgg
     6121 tggatctgca gcaggggatt cagattttcg agctgcgtat ttacgccgct gagatgggtc
     6181 accgtatgcc gctacgccat gagattcacc agctgattct ggctggcttc cacgcccacg
     6241 gtatcgatat gccattcccg cccttccaga tgcgtctgga aagcctgaat ggtaaacaaa
     6301 cggggagaac attaacttct gcgggcaaag gtcgtcaggc gggaagtttg taaggtcttt
     6361 gtggccggat gaacccatcc ggctcacaag caaaactgat agcgcagaaa aaactgccac
     6421 aaccagtagc cgaaaatagc cagcgcggtg gcgtacataa accagccgcg tttttttcgc
     6481 cacaaagcga accagagaaa agggaaaaag agcggtaacg tcattatcac accgacatcg
     6541 cgagtatcgt catcttcgag atacatcaaa acatcacaga cgttgttaat cccgcgtcca
     6601 tcaatcatcc actctttgtc cagcgcggag gtaatggtta tccagaggaa cgccacaatg
     6661 aaccagatgc agcacaagac agacatgcgc gcactcatga ggtaacccat agtttaattt
     6721 cgctatagcg catacgaaat ctcactaatt ccctgtcatt acgccctatc gtcaggccga
     6781 cgatagggca tttgattact cagccaccca ctgcggattt tttaccactc tgctgcaata
     6841 gttctggtaa atcgccatcg ctaacagcga gaagaagaca gggccgccaa tcatccacaa
     6901 tgtgctgtcc cagtctccgg cttcaaccac aggttgaatg atggtgaaga cgttcgcaaa
     6961 tgtcaccacc agtacgacaa ccactgtcgc aatcattgcc gacatacgcg ttttgaaaat
     7021 cacaaacggt ctgtcgagat cctgacgtgc tttaaagaac gggaaagcca gcgcgaggaa
     7081 caggtaagga agcgtcatag acacgttcgc catcagcgtc agcttgttaa agaacgccga
     7141 tgcggtaccg ccaccaaacg aaaccagcag gatgaagaca gtaaccaacc cacactgcat
     7201 ccacatggcg atagacggca tccccatcgc attcaggcgc gtcatcggtt ccggccacaa
     7261 tgctttcggc gtcccctgga tgatggcttt cagcggtgaa tagcaaagcg taaagaacgc
     7321 accggtataa gcgaggaaca tcgacagtcc ggtaatacgc gcaaaccata cgcccagcga
     7381 caatgacgct tcaggtgaca aatgcagtgc gttacccagc gtcaccccga ggctcttcat
     7441 cagcacatag gtaatattgc cgaggttaac ggaaccatta cttaatacct gctgccagtt
     7501 tgtgctgacg ccccataaaa atattgccag cgaataaccg attgaaataa caatagcggc
     7561 aaaaacaata cctttggcaa agttcttttc tggattttcc gttttatcaa ccagaccacc
     7621 aaccgcttca attccgccat aggcaaaaat ggcaaatacc acaaacgata gcattgccag
     7681 accggactga taacctgggt tcggtgatgc aaggaaatta atatcctgcg cgaaatgccc
     7741 accatttaat aacaaaatag taatgcttac taacagcaat actaaattca gacacattac
     7801 tgcaataccg cccaccgcag taatgcgggc aattttatta atcccttttg aagcaacgac
     7861 ggtgaccaga atcatccatg ccactgccag cagaccaacc acctgcgtag gctccagtcc
     7921 ggcaatatgc cagtgctggg tcatgtcgct accgtagagg aatgttgaga acggtaccca
     7981 aactttcgcg gaggtactca ccatccagat gatataagag gaaaaccaca taaacgtacc
     8041 aatgaaggca aaacgtggtc cgacactatt attcatccag gaatagatac cgccttcttc
     8101 tttgcgataa gcagctccca tttcagccat cattaaggcg aatggaataa agaataacaa
     8161 tgcagaaaat atataaaagg aaatcgcact ataacccatt aagtaataag ccgatgggct
     8221 attggcaaat ccaaatacag aagtaaagat catcaatatg agtcctatca gactcatctt
     8281 ttttatcgag tgaggcatga aaccatcctt catttcagcg cgtgtataaa acgcgcccac
     8341 tgaactgcac tgcccaacat tgagcgggca gcgtaaaaac caatacagaa ttgatacctg
     8401 gatgatatca aatatccagt cttaaattgt ggcctttagc ggaaaaatga aaacatatgc
     8461 tacagaatgg cgcttatcac gccattcttc gctgttaatt cagtaatttt tcagaattat
     8521 gcccggtcaa cgctaaaggc gatgacttca gccagtgtct ccgcgcccag cgccaacatc
     8581 accagacgat caacacctaa tgccacgccg gaacagtcag gcataccgac tttcaaggct
     8641 tcaatcagat tctggtcaat ggggtgctgc ggcaaaccgc gcgccgcgcg cttacggtta
     8701 tcttgttcaa agcgttgttg ctgctcacgg gcatccgtca attcatggaa accattcgcc
     8761 agctcaatac ctttataata aacctcaaag cgttcagcga cccgatgatc ttcggtactg
     8821 atttgcgcca gtgatgcctg gctggctgga aagtggtaca caaaggtcgg tttttctttg
     8881 ccaatatttg gctctacgcc aaaggtaaac agcaattgta gaagcgtgtc gcggtcttct
     8941 tcagtatcgg caacgttgct caaatccagt ttcgctgcca cttcccgcag ttgcgttttg
     9001 tcggcagaga gtgggtcaat ttccagataa cgcaagaaag cttgttgata agaaaggctt
     9061 tctgctgccg gacagtccag cacctgttgc aagagatcgt ccacctcgtt catcaaccgg
     9121 tacatatcat agtgcggtcg ataccactca agcatagtga actcagggtt gtgataacgc
     9181 cccatctctt cattacggaa gctgcggcac agctggaata ccggcccaca accggcaacc
     9241 agcaggcgct tcatatggta ttccgggctg gtcattaacc agagattcat cccctgcgaa
     9301 tgcccggggc caacgaaacg tgtctcaaac gggaccaaat gaatatcggt taccgtcgcc
     9361 tggctcatac aaggcgtctc cacctccagc actccacgat cggcaaagaa acgacggatc
     9421 tccgccataa tcgccgcgcg ttttaataag ttaggaatgg atgcgctcgg ctgccaggat
     9481 gccgtttcgc tcatagttaa atctccagtt tttgacaagg gcacgaagtc tactcgcaac
     9541 gcgacggcga gacaaatttt acgcaggaat aacacaacgg tgggcagtga ctaaaaaaag
     9601 cacgatctga tggtttagta attaaattaa tcatcttcag tgataattta gccctcttgc
     9661 gcactaaaaa aatcgatctc gtcaaatttc agacttatcc atcagactat actgttgtac
     9721 ctataaagga gcagtggaat agcgttcgca gaccgtaact ttcaggcact taccctgaag
     9781 tacggggctg tgggataaaa acaatctgga ggaatgtcgt gcaaaccttt caagccgatc
     9841 ttgccattgt aggcgccggt ggcgcgggat tacgtgctgc aattgctgcc gcgcaggcaa
     9901 atccaaatgc aaaaatcgca ctaatctcaa aagtataccc gatgcgtagc cataccgttg
     9961 ctgcagaagg gggctccgcc gctgtcgcgc aggatcatga cagcttcgaa tatcactttc
    10021 acgatacagt agcgggtggc gactggttgt gtgagcagga tgtcgtggat tatttcgtcc
    10081 accactgccc aaccgaaatg acccaactgg aactgtgggg gtgcccatgg agccgtcgcc
    10141 cggatggtag cgtcaacgta cgtcgcttcg gcggcatgaa aatcgagcgc acctggttcg
    10201 ccgccgataa gaccggcttc catatgctgc acacgctgtt ccagacctct ctgcaattcc
    10261 cgcagatcca gcgtttcgac gaacatttcg tgctggatat tctagttgat gatggtcatg
    10321 ttcgcggcct ggtagcaatg aacatgatgg aaggcacgct ggtgcagatc cgtgctaacg
    10381 cggtcgttat ggctaccggc ggtgcaggtc gcgtttatcg ttacaacacc aacggcggca
    10441 tcgttaccgg tgacggtatg ggtatggcgc taagccacgg cgttccgctg cgtgacatgg
    10501 aattcgttca gtatcaccca accggtctac caggttccgg tatcctgatg accgaaggct
    10561 gccgcggtga aggcggtatt ctggtcaaca aaaatggcta ccgttatctg caagattacg
    10621 gcatgggccc ggaaactccg ctgggcgagc cgaaaaacaa atatatggaa ctgggtccac
    10681 gcgacaaagt ttctcaggcc ttctggcacg aatggcgtaa aggcaacacc atctccacgc
    10741 cgcgtggcga tgtggtttat ctcgacctgc gtcaccttgg cgagaaaaaa ctgcatgaac
    10801 gtttgccgtt catctgcgaa ctggcgaaag cgtacgttgg cgtcgatccg gttaaagaac
    10861 cgattccggt acgtccgacc gcacactaca ccatgggcgg tatcgaaacc gatcagaact
    10921 gtgaaacccg cattaaaggt ctgttcgccg tgggtgaatg ttcctctgtt ggtctgcacg
    10981 gtgcgaaccg tctgggctcc aactcgctgg cggaactggt ggtcttcggt cgtctggccg
    11041 gtgaacaagc gacagagcgt gcagcaactg ccggtaatgg caacgaagcg gcaattgaag
    11101 cgcaggcagc tggcgttgaa caacgtctga aagatctggt taaccaggat ggcggcgaaa
    11161 actgggctaa gatccgcgac gaaatgggca tggccatgga agaaggctgc ggtatctacc
    11221 gtacgccgga actgatgcag aaaaccatcg acaagctggc agagctgcag gaacgcttca
    11281 agcgcgtgcg catcaccgac acttccagcg tgttcaacac cgacctgctc tacaccattg
    11341 agctgggcca cggtctgaac gttgctgaat gtatggcgca ctccgcaatg gcacgtaaag
    11401 agtcccgcgg cgcgcaccag cgtctggacg aaggttgcac cgagcgtgac gacgtcaact
    11461 tcctcaaaca caccctcgcc ttccgcgatg ctgatggcac gactcgcctg gagtacagcg
    11521 acgtgaagat tactacgctg ccgccagcta aacgtgttta cggtggcgaa gcggatgcag
    11581 ccgataaggc ggaagcagcc aataagaagg agaaggcgaa tggctgagat gaaaaacctg
    11641 aaaattgagg tggtgcgcta taacccggaa gtcgataccg caccgcatag cgcattctat
    11701 gaagtgcctt atgacgcaac tacctcatta ctggatgcgc tgggctacat caaagacaac
    11761 ctggcaccgg acctgagcta ccgctggtcc tgccgtatgg cgatttgtgg ctcctgcggc
    11821 atgatggtta acaacgtgcc aaaactggca tgtaaaacct tcctgcgtga ttacaccgac
    11881 ggtatgaagg ttgaagcgtt agctaacttc ccgattgaac gcgatctggt ggtcgatatg
    11941 acccacttca tcgaaagtct ggaagcgatc aaaccgtaca tcatcggcaa ctcccgcacc
    12001 gcggatcagg gtactaacat ccagaccccg gcgcagatgg cgaagtatca ccagttctcc
    12061 ggttgcatca actgtggtct gtgctacgcc gcgtgcccgc agtttggcct gaacccagag
    12121 ttcatcggtc cggctgccat tacgctggcg catcgttata acgaagatag ccgcgaccac
    12181 ggtaagaagg agcgtatggc gcagttgaac agccagaacg gcgtatggag ctgtactttc
    12241 gtgggctact gctccgaagt ctgcccgaaa cacgtcgatc cggctgcggc cattcagcag
    12301 ggcaaagtag aaagttcgaa agactttctt atcgcgaccc tgaaaccacg ctaaggagtg
    12361 caacatgacg actaaacgta aaccgtatgt acggccaatg acgtccacct ggtggaaaaa
    12421 attgccgttt tatcgctttt acatgctgcg cgaaggcacg gcagttccgg ctgtgtggtt
    12481 cagcattgaa ctgattttcg ggctgtttgc cctgaaaaat ggcccggaag cctgggcggg
    12541 attcgtcgac tttttacaaa acccggttat cgtgatcatt aacctgatca ctctggcggc
    12601 agccctactg cacaccaaaa cctggtttga gctggcacca aaagcggcca atatcattgt
    12661 aaaagacgaa aaaatgggac cagagccaat tatcaaaagt ctctgggcgg taactgtggt
    12721 tgccaccatc gtaatcctgt ttgttgccct gtactggtaa ggagcctgag atgattaatc
    12781 caaatccaaa gcgttctgat gaaccggtat tctggggcct cttcggggcc ggtggtatgt
    12841 ggagcgccat cattgcgccg gtgatgatcc tactggttgg tattctgcta ccgctagggc
    12901 tgtttccagg tgatgcgctg agctacgagc gcgttctggc gttcgcgcag agcttcattg
    12961 gtcgcgtatt cctgttcctg atgatcgttc tgccgctgtg gtgtggttta caccgaatgc
    13021 accacgcgat gcacgatctg aaaatccacg tacctgcggg taaatgggtt ttctacggtc
    13081 tggctgctat cctgacagtt gtcacactga ttggtgtcgt tacaatctaa cgcatcgcca
    13141 atgtaaatcc ggcccgccta tggcgggccg ttttgtatgg aaaccagacc ctatgttcaa
    13201 aacgacgctc tgcaccttat taattaccgc ctcttgctcc acatttgccg cccctcaaca
    13261 aatcaacgat attgtgcatc gcacaattac cccgcttata gagcaacaaa ggatccccgg
    13321 tatggcggta gcggtaattt atcagggtaa accttattac tttacctggg gctatgcgga
    13381 catcgccaaa aagcagcccg tcacacagca aacgttgttt gagttaggtt cggtcagcaa
    13441 aacatttacg ggcgtgcttg gtggcgacgc tattgctcga ggggaaatca agttaagcga
    13501 tcccacaaca aaatactggc ctgaacttac cgctaaacag tggaatggga tcacactatt
    13561 acatctcgca acctacaccg ctggcggcct gccattgcag gtgccggatg aggtgaaatc
    13621 ctcaagcgac ttgctgcgct tctatcaaaa ctggcagcct gcatgggctc caggaacaca
    13681 acgtctgtat gccaactcca gtatcggttt gttcggcgca ctggctgtga agccgtctgg
    13741 tttgagtttt gagcaggcga tgaaaactcg tgtcttccag ccactcaaac tcaatcatac
    13801 gtggattaat gtaccgtccg cagaagaaaa gaactacgcc tggggatatc gcgaaggtaa
    13861 ggcagtacat gtttcgccag gggcgttaga tgctgaagct tatggtgtga agtcgaccat
    13921 tgaagatatg gcccgctggg tgcaaagcaa tttaaaaccc cttgatatca atgagaaaat
    13981 acttcaacaa gggatacaac tggcacaatc tcgctactgg caaaccggcg atatgtatca
    14041 gggtctgggc tgggaaatgc tggactggcc ggtaaatcct gacatcatca ttaacggcag
    14101 tgacaataaa attgcactgg cagcacgccc cgtaaaaccg attacgcccc caactcctgc
    14161 agtacgcgca tcatgggtac ataaaacggg ggcgaccggc ggatttggta gctatgtcgc
    14221 gtttattcca gaaaaagagc tgggtatcgt gatgctggct aacaaaaact atcccaatcc
    14281 agcgagagtt accgccgcct ggcagattct caacactcta cagtaaaatt tcatcgggtc
    14341 cgaattttcg gaccttttct ccgcttttcc ttgctgtcat ctacacttag aaaaaaacca
    14401 gtaaggaaac atttatgcgc ctgctccctc tcgttgccgc agcgacagct gcatttctgg
    14461 tcgttgcctg cagttctcct acaccgccgc gtggcgtgac cgtagtgaat aatttcgacg
    14521 ctaaacgcta tctcggcacc tggtatgaga ttgcccgttt tgatcaccgc tttgaacgtg
    14581 gactggaaaa agtcaccgca acatacagcc tgcgtgatga cggcggcctg aatgtcatta
    14641 ataaaggcta taaccctgac agagaaatgt ggcagcagag tgaagggaaa gcgtacttta
    14701 ccggcgcacc aactcgcgct gcgctgaaag tgtcattctt tggtcctttc tatggcggtt
    14761 ataacgttat tgcactcgat cgggaatacc gccatgcgct ggtttgcggc ccggaccgcg
    14821 actacctgtg gatactctcc cgcacgccaa ccatttctga cgaagtgaaa caggagatgc
    14881 tggcagtcgc gacccgggaa gggtttgatg tcagtaagtt tatttgggta cagcagcccg
    14941 gtagttaatg agtgctgagt ttcagaccaa taatccccaa tacgattaac gccagactcg
    15001 ccagacgcat cgggttagcg gactcaccga gcaacacaat gccggtgatg gccgcgccga
    15061 ctgcgccaat acccgtccac acggcataag ccgtccctac tggtaacgat ttcatcgccc
    15121 aggcaagtag cgccatactg acaatcatcg ccgtcacggt aataacactt ggcgtcaaac
    15181 gactaaagcc gtgggtatat ttcaggccaa cggcccatac cacttccagc agaccagcaa
    15241 taactaagat aatccaggac atatcaggct ccggaaaatt ggggccgtcc ccggtgaaaa
    15301 gacgcgtttg caggtcgtcc tgcaaagctg atggttgaac ggatattttg ccaggtggca
    15361 ggggaatttc aactattacc tatgtaacta tcgcagaaaa aacaaacggc acgacacagc
    15421 tgtcgtaccg ttattgctta ttgctgcgct ttcgttgctg cgccagaaat cgcgttaccg
    15481 ccatcagaaa tgtcttcacc aacgccacgc gtggtgttgc aggcagttaa tactgttgaa
    15541 agcaccagaa cagaaaagat cgctgcaatt gtcttcttca ccataacgtc ttccttttag
    15601 gtaatgttat ttatgtttgc ctatagcaaa ttaataatag ac
//
LOCUS       DAHHTG010000414        10046 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.guided.148, whole genome shotgun sequence.
ACCESSION   DAHHTG010000414 DAHHTG010000000
VERSION     DAHHTG010000414.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10046)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10046)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10046
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.guided.148"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            complement(36..785)
                     /gene="intI1"
                     /locus_tag="NBI74_004811"
                     /pseudo
     CDS             complement(36..785)
                     /gene="intI1"
                     /locus_tag="NBI74_004811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221021.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 integron integrase IntI1"
     gene            943..1416
                     /locus_tag="NBI74_004812"
     CDS             943..1416
                     /locus_tag="NBI74_004812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001389366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrA17"
                     /protein_id="HCD1935000.1"
                     /translation="MKISLISAVSENGVIGSGPDIPWSVKGEQLLFKALTYNQWLLVG
                     RKTFDSMGVLPNRKYAVVSKNGISSSNENVLVFPSIENALKELSKVTDHVYVSGGGQI
                     YNSLIEKADIIHLSTVHVEVEGDIKFPIMPENFNLVFEQFFMSNINYTYQIWKKG"
     gene            1547..2335
                     /locus_tag="NBI74_004813"
     CDS             1547..2335
                     /locus_tag="NBI74_004813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000503573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase AadA5"
                     /protein_id="HCD1935001.1"
                     /translation="MGEFFPAQVFKQLSHARAVIERHLAATLDTIHLFGSAIDGGLKP
                     DSDIDLLVTVSAAPNDSLRQALMLDLLKVSSPPGDGGTWRPLELTVVARSEVVPWRYP
                     ARRELQFGEWLRHDILSGTFEPAVLDHDLAILLTKARQHSLALLGPSAATFFEPVPKE
                     HFSKALFDTIAQWNAESDWKGDERNVVLALARIWYSASTGLIAPKDVAAAWVSERLPA
                     EHRPLICKARAAYLGSEDDDLAMRVEETAAFVRYAKATIERILR"
     gene            2541..2888
                     /locus_tag="NBI74_004814"
     CDS             2541..2888
                     /locus_tag="NBI74_004814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000679427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter QacE delta 1"
                     /protein_id="HCD1935002.1"
                     /translation="MKGWLFLVIAIVGEVIATSALKSSEGFTKLAPSAVVIIGYGIAF
                     YFLSLVLKSIPVGVAYAVWSGLGVVIITAIAWLLHGQKLDAWGFVGMGLIIAAFLLAR
                     SPSWKSLRRPTPW"
     gene            2882..3721
                     /gene="sul1"
                     /locus_tag="NBI74_004815"
     CDS             2882..3721
                     /gene="sul1"
                     /locus_tag="NBI74_004815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000259031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonamide-resistant dihydropteroate synthase
                     Sul1"
                     /protein_id="HCD1935003.1"
                     /translation="MVTVFGILNLTEDSFFDESRRLDPAGAVTAAIEMLRVGSDVVDV
                     GPAASHPDARPVSPADEIRRIAPLLDALSDQMHRVSIDSFQPETQRYALKRGVGYLND
                     IQGFPDPALYPDIAEADCRLVVMHSAQRDGIATRTGHLRPEDALDEIVRFFEARVSAL
                     RRSGVAADRLILDPGMGFFLSPAPETSLHVLSNLQKLKSALGLPLLVSVSRKSFLGAT
                     VGLPVKDLGPASLAAELHAIGNGADYVRTHAPGDLRSAITFSETLAKFRSRDARDRGL
                     DHA"
     gene            3849..4052
                     /locus_tag="NBI74_004816"
     CDS             3849..4052
                     /locus_tag="NBI74_004816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000376616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1935004.1"
                     /translation="MDSEEPPNVRVACSGDIDEVVRLMHDAAAWMSAKGTPAWDVARI
                     DRTFAETFVLRSELLGIASENGK"
     gene            4208..5413
                     /gene="chrA"
                     /locus_tag="NBI74_004817"
     CDS             4208..5413
                     /gene="chrA"
                     /locus_tag="NBI74_004817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004896675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromate efflux transporter"
                     /protein_id="HCD1935005.1"
                     /translation="MTNNPTDDSRPWSVFLIFLRLGLTSFGGPIAHLGYFRAEFVTRR
                     RWLSERSYADLVALCQFLPGPASSQVGIAVGLSRAGYSGALAAWAGFTLPSAIALILF
                     ALGISSYGDYVSQGALHGLKVVAVAVVAQAVWGMARNLCTDGLRVTIMAIATCVVLLV
                     PSAWGQVGVIAIAGIAGRLLFKPAKVVEHDPLPITVSHRAGVLWLSLFFVLLIGLPVL
                     AELMPSQTMAMVDSFYRVGSLVFGGGHVVLPLLQAEVVPSGWVNNESFLAGYGAAQAV
                     PGPLFTFAAFLGASMNTAPSGWIGGIVCLLAIFAPSFLLVVGSMPFWERLRRNTGIQA
                     ALAGINAAVVGLLLAALYQPVWTSAIFQPQDFGLALVALVALMFWKLPPWLVVLGSGA
                     AGWLLSVAL"
     gene            5424..5729
                     /locus_tag="NBI74_004818"
     CDS             5424..5729
                     /locus_tag="NBI74_004818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000130000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCD1935006.1"
                     /translation="MTDKDLYGGLIRLHILHHAAEEPVFGLGIIEELRRHGYEMSAGT
                     VYPMLHGLEKKGYLTSRHERTGRRERRVYDITEQGRTALADAKTKVKELFGELVEGG"
     gene            5773..5838
                     /locus_tag="NBI74_004819"
                     /pseudo
     CDS             5773..5838
                     /locus_tag="NBI74_004819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229646.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
     gene            5956..6720
                     /locus_tag="NBI74_004820"
     CDS             5956..6720
                     /locus_tag="NBI74_004820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001389365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS6100 family transposase"
                     /protein_id="HCD1935007.1"
                     /translation="MTDFKWRHFQGDVILWAVRWYCRYPISYRDLEEMLAERGISVDH
                     TTIYRWVQCYAPEMEKRLRWFWRRGFDPSWRLDETYVKVRGKWTYLYRAVDKRGDTID
                     FYLSPTRSAKAAKRFLGKALRGLKHWEKPATLNTDKAPSYGAAITELKREGKLDRETA
                     HRQVKYLNNVIEADHGKLKILIKPVRGFKSIPTAYATIKGFEVMRALRKGQARPWCLQ
                     PGIRGEVRLVERAFGIGPSALTEAMGMLNHHFAAAA"
     gene            complement(7213..7797)
                     /locus_tag="NBI74_004821"
     CDS             complement(7213..7797)
                     /locus_tag="NBI74_004821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001137892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCD1935008.1"
                     /translation="MPRPKLKSDDEVLEAATVVLKRCGPIEFTLSGVAKEVGLSRAAL
                     IQRFTNRDTLLVRMMERGVEQVRHYLNAIPIGAGPQGLWEFLQVLVRSMNTRNDFSVN
                     YLISWYELQVPELRTLAIQRNRAVVEGIRKRLPPGAPAAAELLLHSVIAGATMQWAVD
                     PDGELADHVLAQIAAILCLMFPEHDDFQLLQAHA"
     gene            complement(7797..9035)
                     /locus_tag="NBI74_004822"
     CDS             complement(7797..9035)
                     /locus_tag="NBI74_004822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCD1935009.1"
                     /translation="MSERRYSPLATLFAATFLFRIGNAVAALALPWFVLSHTKSAAWA
                     GATAASSVIATIIGAWVGGGLVDRFGRAPVALISGVVGGVAMASIPLLDAVGALSNTG
                     LIACVVLGAAFDAPGMAAQDSELPKLGHVAGLSVERVSSLKAVIGNVAILGGPALGGA
                     AIGLLGAAPTLGLTAFCSVLAGLLGAWVLPARAARTMTTTATLSMRAGVAFLWSEPLL
                     RPLFGIVMIFVGIVGANGSVIMPALFVDAGRQVAELGLFSSMMGAGGLLGIAIHASVG
                     ARISAQNWLAVAFCGSAVGSLLLSQLPGVPVLMLLGALVGLLTGSVSPILNAAIYNRT
                     PPELLGRVLGTVSAVMLSASPMVMLAAGAFVDLAGPLPGLVVSAVFAGLVALLSLRLQ
                     FATMAAAATASAPTHTEGEH"
     gene            complement(9032..9937)
                     /locus_tag="NBI74_004823"
     CDS             complement(9032..9937)
                     /locus_tag="NBI74_004823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000219391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mph(A) family macrolide 2'-phosphotransferase"
                     /protein_id="HCD1935010.1"
                     /translation="MTVVTTADTSQLYALAARHGLKLHGPLTVNELGLDYRIVIATVD
                     DGRRWVLRIPRRAEVSAKVEPEARVLAMLKNRLPFAVPDWRVANAELVAYPMLEDSTA
                     MVIQPGSSTPDWVVPQDSEVFAESFATALAALHAVPISAAVDAGMLIRTPTQARQKVA
                     DDVDRVRREFVVNDKRLHRWQRWLDDDSSWPDFSVVVHGDLYVGHVLIDNTERVSGMI
                     DWSEARVDDPAIDMAAHLMVFGEEGLAKLLLTYEAAGGRVWPRLAHHIAERLAFGAVT
                     YALFALDSGNEEYLAAAKAQLAAAE"
BASE COUNT         2092 a         2900 c         2956 g         2098 t
ORIGIN      
        1 gatgataagt ttatcaccac cgactatttg caacagtgcc gacgcacgac accgctccgt
       61 ggatcggtcg aatgcgtgtg ctgcgcaaaa acccagaacc acagccagga atgcccggcg
      121 cgcggatact tccgctcaag ggcgtcggga agcgcaacgc cgctgcggcc ctcggcctgg
      181 tccttcagcc accatgcccg tgcacgcgac agctgctcgc gcaggctggg tgccaagctc
      241 tcgggtaaca tcaaggcccg atccttggag cccttgccct cccgcacgat gatcgtgccg
      301 tgatcgaaat ccagatcctt gacccgcagt tgcaaaccct cactgatccg catgcccgtt
      361 ccatacagaa gctgggcgaa caaacgatgc tcgccttcca gaaaaccgag gatgcgaacc
      421 acttcatccg gggtcagcac caccggcaag cgccgcgacg gccgaggtct tccgatctcc
      481 tgaagccagg gcagatccgt gcacagcacc ttgccgtaga agaacagcaa ggccgccaat
      541 gcctgacgat gcgtggagac cgaaaccttg cgctcgttcg ccagccagga cagaaatgcc
      601 tcgacttcgc tgctgcccaa ggttgccggg tgacgcacac cgtggaaacg gatgaaggca
      661 cgaacccagt ggacataagc ctgttcggtt cgtaaactgt aatgcaagta gcgtatgcgc
      721 tcacgcaact ggtccagaac cttgaccgaa cgcagcggtg gtaacggcgc agtggcggtt
      781 ttcatggctt gttatgactg tttttttgta cagtctatgc ctcgggcatc caagcagcaa
      841 gcgcgttacg ccgtgggtcg atgtttgatg ttatggagca gcaacgatgt tacgcagcag
      901 ggcagtcgcc ctaaaacaaa gttagccatt aagggagtta aattgaaaat atcattgatt
      961 tctgcagtgt cagaaaatgg cgtaatcggt agtggtcctg atatcccgtg gtcagtaaaa
     1021 ggtgagcaac tactctttaa agcgctcaca tataatcaat ggctccttgt cggaagaaaa
     1081 acatttgact ctatgggtgt tcttccaaat cgcaaatatg cagtagtgtc aaagaacgga
     1141 atttcaagct caaatgaaaa cgtcctagtt tttccttcaa tagaaaatgc tttgaaagag
     1201 ctatcaaaag ttacagatca tgtatatgtc tctggcgggg gtcaaatcta taatagcctt
     1261 attgaaaaag cagatataat tcatttgtct actgttcacg ttgaagtcga aggtgatatc
     1321 aaattcccta taatgcctga gaatttcaat ttggtttttg aacagttttt tatgtctaat
     1381 ataaattata cataccagat ttggaaaaaa ggctaacaat gcgttgcagc accagtcgct
     1441 tcgctccttg gacagctttt aagtcgcgtc tttgtggttt tgctgcgcaa aagtattcca
     1501 caaagccgca acttaaaagc tgccgctgaa cttaacgtta ggcatcatgg gtgaattttt
     1561 ccctgcacaa gttttcaagc agctgtccca cgctcgcgcg gtgatcgagc gccatctggc
     1621 tgcgacactg gacacaatcc acctgttcgg atctgcgatc gatggagggc tgaagccgga
     1681 cagcgacata gacttgctcg tgaccgtcag cgccgcacct aacgattcgc tccggcaggc
     1741 gctaatgctc gatttgctga aagtctcatc accgccaggc gatggcggaa catggcgacc
     1801 gctggagcta actgttgtcg ctcgaagcga agtagtgcct tggcgctatc cggcgcggcg
     1861 tgagcttcag ttcggtgagt ggctccgcca cgacatcctt tccggaacgt tcgagcctgc
     1921 cgttctggat cacgatcttg cgattttgct gaccaaggcg aggcaacaca gccttgcgct
     1981 tctaggccca tccgcagcca cgtttttcga gccggtgccg aaggagcatt tctccaaggc
     2041 gcttttcgac actattgccc agtggaatgc agagtcggat tggaagggtg acgagcggaa
     2101 cgtcgttctt gctcttgctc gcatttggta cagcgcttca actggtctca ttgctcctaa
     2161 ggacgttgct gccgcatggg tatcggagcg tttgcctgcc gagcatcggc ccctcatctg
     2221 caaggcacgc gcggcgtacc tgggtagcga ggacgacgac ctagcaatgc gcgtcgaaga
     2281 gacggccgcg ttcgttcgat atgccaaagc aacgattgag agaatcttgc gttgagcggc
     2341 atgtgcgaaa agtgcatcga cccgcgccga gggcatctga tgcctaactc ggcgttcaag
     2401 cggacgggct gcgcccgccg ctcaactatg cgttagatgc actaagcaca taattgctca
     2461 cagccaaact atcaggtcaa gtctgctttt attattttta agcgtgcata ataagcccta
     2521 cacaaattgg gagatatatc atgaaaggct ggctttttct tgttatcgca atagttggcg
     2581 aagtaatcgc aacatccgca ttaaaatcta gcgagggctt tactaagctt gccccttccg
     2641 ccgttgtcat aatcggttat ggcatcgcat tttattttct ttctctggtt ctgaaatcca
     2701 tccctgtcgg tgttgcttat gcagtctggt cgggactcgg cgtcgtcata attacagcca
     2761 ttgcctggtt gcttcatggg caaaagcttg atgcgtgggg ctttgtaggt atggggctca
     2821 taattgctgc ctttttgctc gcccgatccc catcgtggaa gtcgctgcgg aggccgacgc
     2881 catggtgacg gtgttcggca ttctgaatct caccgaggac tccttcttcg atgagagccg
     2941 gcggctagac cccgccggcg ctgtcaccgc ggcgatcgaa atgctgcgag tcggatcaga
     3001 cgtcgtggat gtcggaccgg ccgccagcca tccggacgcg aggcctgtat cgccggccga
     3061 tgagatcaga cgtattgcgc cgctcttaga cgccctgtcc gatcagatgc accgtgtttc
     3121 aatcgacagc ttccaaccgg aaacccagcg ctatgcgctc aagcgcggcg tgggctacct
     3181 gaacgatatc caaggatttc ctgaccctgc gctctatccc gatattgctg aggcggactg
     3241 caggctggtg gttatgcact cagcgcagcg ggatggcatc gccacccgca ccggtcacct
     3301 tcgacccgaa gacgcgctcg acgagattgt gcggttcttc gaggcgcggg tttccgcctt
     3361 gcgacggagc ggggtcgctg ccgaccggct catcctcgat ccggggatgg gatttttctt
     3421 gagccccgca ccggaaacat cgctgcacgt gctgtcgaac cttcaaaagc tgaagtcggc
     3481 gttggggctt ccgctattgg tctcggtgtc gcggaaatcc ttcttgggcg ccaccgttgg
     3541 ccttcctgta aaggatctgg gtccagcgag ccttgcggcg gaacttcacg cgatcggcaa
     3601 tggcgctgac tacgtccgca cccacgcgcc tggagatctg cgaagcgcaa tcaccttctc
     3661 ggaaaccctc gcgaaatttc gcagtcgcga cgccagagac cgagggttag atcatgccta
     3721 gcattcacct tccggccgcc cgctagcgga ccctggtcag gttccgcgaa ggtgggcgca
     3781 gacatgctgg gctcgtcagg atcaaactgc actatgaggc ggcggttcat accgcgccag
     3841 gggagcgaat ggacagcgag gagcctccga acgttcgggt cgcctgctcg ggtgatatcg
     3901 acgaggttgt gcggctgatg cacgacgctg cggcgtggat gtccgccaag ggaacgcccg
     3961 cctgggacgt cgcgcggatc gaccggacat tcgcggagac cttcgtcctg agatccgagc
     4021 tcctagggat cgcctcagaa aacggaaaat aaagcacgct aagccgtaag taagcgtgct
     4081 cctgtgaaag ccacagctaa aactgcgtag tacacataga ggtatttttt tctagattta
     4141 tatcgaattt cgttatcgaa tttcgcttat tattgatcaa ctcgttgcag atcatgaagg
     4201 cgaaagtatg acgaataacc ctaccgatga cagcagacca tggtcggtct ttcttatttt
     4261 tctgcggctt ggattgacat cttttggcgg ccccattgcg cacttgggct acttccgcgc
     4321 cgaatttgtc acacggcggc gctggctctc cgaacggagc tatgctgact tggtcgcgct
     4381 ttgtcagttc ttgccagggc ctgcaagcag ccaggtcggc atagcggtag gactgtctcg
     4441 ggctggatac agcggggcgc tggctgcttg ggctggcttc acgctgccgt ctgccatagc
     4501 cttgatcctt tttgcgctcg gcatctccag ctatggcgat tacgtctcgc agggcgcgtt
     4561 gcatggctta aaagtggtgg ctgtggccgt ggtcgctcaa gcagtatggg gcatggcgcg
     4621 taacctatgc acggatgggc tgcgagtcac catcatggca attgctacct gcgtcgtttt
     4681 acttgtgccg tccgcgtggg gacaggttgg cgtgattgct atcgcaggca tcgcaggccg
     4741 gttattgttc aagccagcga aagttgttga gcatgacccc ctacctatca cggtcagtca
     4801 ccgggccggc gtgctttggc tctcgctgtt ctttgtcttg ctgattggcc tgccggtgtt
     4861 ggccgaactg atgccaagtc aaaccatggc aatggtggat tccttctatc gtgtcggatc
     4921 actggtgttc ggcggtggtc acgttgtgct gccattactg caagccgaag tggtgccctc
     4981 cggctgggtc aacaatgaat cctttctcgc ggggtacggg gcagctcaag cggtgcccgg
     5041 ccctttgttc acgttcgccg cgtttcttgg tgcctcgatg aacaccgccc cgtcgggctg
     5101 gatcggcggc attgtgtgtc tgctggctat cttcgcgccc tcgttcttgc tggtcgtcgg
     5161 atcaatgcca ttttgggagc gtttgcgccg caatacaggc atccaagctg cgctggccgg
     5221 gatcaatgcc gctgtagtcg gcttgctgct ggccgcgctg tatcagcctg tatggactag
     5281 cgccatcttt cagccgcaag acttcggctt ggcattagtt gcccttgtcg cacttatgtt
     5341 ctggaagctc ccgccgtggc tggttgtcct cggtagcggt gcagctgggt ggctgttgag
     5401 cgtcgctctg tgaggcacaa aaaatgactg acaaagacct ctacggcggt ttgatccgcc
     5461 tgcacatcct tcaccatgca gccgaggaac ctgtctttgg gctggggatc atcgaagagc
     5521 tacgccgaca cggctacgag atgagcgctg gcaccgtgta cccgatgctg cacggcctgg
     5581 aaaaaaaagg ctatctgacc tcacgccacg aacgcaccgg gcgacgtgaa cggcgtgttt
     5641 atgacatcac tgaacaaggc agaactgcac ttgctgatgc gaaaacaaag gtcaaggagc
     5701 tgttcggaga gttggtagaa ggtggttgag tttttgcttc tatgtcaggt tgagctatac
     5761 cctaactgga ttgtcatttt cagaagacga ctgcaccagt tgattgggcg taatggctgt
     5821 tgtgcagcca gctcctgaca gttcaatatc agaagtgatc tgcaccaatc tcgactatgc
     5881 tcaatactcg tgtgggctct gttgcaaaaa tcgtgaagct tgagcatgct tggcggagat
     5941 tggacggacg gaacgatgac ggatttcaag tggcgccatt tccagggtga tgtgatcctg
     6001 tgggcggtgc gctggtattg tcgctatccg atcagctatc gcgaccttga ggaaatgctg
     6061 gcggaacgcg gcatttcggt cgaccatacg acgatctatc gctgggtcca gtgctacgcc
     6121 ccggagatgg agaagcggct gcgctggttc tggcggcgtg gctttgatcc gagctggcgc
     6181 ctggatgaaa cctacgtcaa ggtgcggggc aagtggacct acctgtaccg ggcagtcgac
     6241 aagcggggcg acacgatcga tttctacctg tcgccgaccc gcagcgccaa ggcagcgaag
     6301 cggttcctgg gcaaggccct gcgaggcctg aagcactggg aaaagcctgc cacgctcaat
     6361 accgacaaag cgccgagcta tggtgcagcg atcaccgaat tgaagcgcga aggaaagctg
     6421 gaccgggaga cggcccaccg gcaggtgaag tatctcaata acgtgatcga ggccgatcac
     6481 ggaaagctca agatactgat caagccggtg cgcggtttca aatcgatccc cacggcctat
     6541 gccacgatca agggattcga agtcatgcga gccctgcgca aaggacaggc tcgcccctgg
     6601 tgcctgcagc ccggcatcag gggcgaggtg cgccttgtgg agagagcttt tggcattggg
     6661 ccctcggcgc tgacggaggc catgggcatg ctcaaccacc atttcgcagc agccgcctga
     6721 tcggcgcaga gcgacagcct acctctgact gccgccaatc tttgcaacag agcccgccgt
     6781 gctagtctgc tcggtgatgg tggagtgaag ccaacccgca atcgggttat gaatctgcat
     6841 cgcgattcgc aatcagctgt ctcttgagca tgtcgaactc ctgcgatgtt atctcgccgt
     6901 gctcgtgctg ctacaccaac ttgtccaact catccgccac accgctcccc accacctgcg
     6961 atggccaggt tgtttgctgg gcgcattggc ggagtatcgt ctcgacccgg gacacccaca
     7021 ccggcacaac cttacgggag tgattcactg tcaaagaatc ggcccggtgc tctgacgcaa
     7081 gtatcgggat ggtcaccatt tgtaagccgt agacctgagt ggtgatcaag acttcgatac
     7141 caccgaccgt accggtacta atcgacgacg gtcgtgttcg tcgcctgccg cagggactct
     7201 gcacacctcc gtttacgcat gtgcctggag gagttggaaa tcgtcgtgtt cgggaaacat
     7261 taaacacagg atggcagcga tctgagccag cacatgatca gctagctcac catccggatc
     7321 gacggcccac tgcatcgtcg cgccagcgat gaccgagtgc aggagcaact cagctgccgc
     7381 aggagcacct gggggcagtc gcttgcggat cccctccacc accgcgcggt tccgctggat
     7441 cgcaagcgtg cgtagctccg gcacctggag ctcgtaccag gagatgagat agttcaccga
     7501 gaagtcgttg cgagtgttca tgctccgaac gagcacctgc aaaaattccc agagcccttg
     7561 cggccctgcg cctatcggta tcgcattcag gtaatgccgc acctgctcga cgccgcgctc
     7621 catcatcctc accagcagcg tatcgcggtt ggtgaagcgc tggattaacg ctgcgcggga
     7681 gagccccacc tcctttgcta ctccgctgag cgtgaactct atgggaccgc aacgcttcag
     7741 cactacggtg gcggcctcga gtacctcgtc atcggacttg agcttggggc ggggcatcag
     7801 tgttcacctt ctgtatgggt tggggcggag gctgtggctg ccgccgccat tgtagcaaat
     7861 tgaagacgga gcgagagtag agccacgagc cccgcaaaca cggccgatac aacgaggcca
     7921 gggagcggac cagcaaggtc gacaaacgcg ccggccgcaa gcataaccat gggcgaggct
     7981 gacagcatca ccgccgagac cgtgccgagt acccggccga gaagttctgg cggcgtgcgg
     8041 ttgtagatgg cagcgttgag aatgggagag actgagccgg tcagcagtcc cacgagcgcg
     8101 cccaacaaca tcagcaccgg cacgcctggc aactgtgaaa gcagaagcga gcccaccgca
     8161 gagccacaaa atgccaccgc cagccagttc tgcgctgata tccgggcgcc gaccgacgca
     8221 tgaatggcaa tgccaaggag accaccagcc cccatcattg aggagaacag cccgagctct
     8281 gctacttggc gtcctgcatc tacaaacagc gcaggcatga tgacgctgcc gttggcgcca
     8341 acgatgccca cgaagatcat cactatacca aagagagggc gcagcagggg ttcgctccag
     8401 agaaaagcga cgccggcgcg catggagaga gtcgccgtcg tggtcatcgt ccgagcggca
     8461 cgcgcgggaa gcacccacgc gccgagcaga cctgcaagga cggagcagaa cgccgtcagc
     8521 ccgagcgttg gcgcagcgcc aagcaggccg attgcggccc ccccaagggc cgggccacct
     8581 agaatcgcga cgttcccgat caccgctttc agtgacgaga cgcgctcaac ggagagcccg
     8641 gcgacgtggc cgagtttggg cagctcactg tcctgcgcgg ccataccggg tgcgtcgaac
     8701 gcggcaccga gcaccacgca agcgatcagc ccagtgttcg agagggcgcc aacggcatcg
     8761 agcagtggga tgctcgccat ggccacgccg cccaccacac ccgagatcaa tgcgacgggc
     8821 gcgcgcccga accgatcgac gaggccacca ccaacccacg cgccgatgat ggtcgcgatg
     8881 acgctgctag cggccgtggc gcccgcccag gccgcgctct ttgtatgaga caggacgaac
     8941 catggaagcg cgagggccgc caccgcgttg ccgatccgga agagaaaggt cgccgcgaac
     9001 agcgtcgcga gcgggctata tcgacgttcg ctcattccgc tgcggcgagc tgcgccttcg
     9061 ccgcagcgag gtactcttcg ttacccgagt cgagggcgaa gagtgcgtag gtgaccgccc
     9121 cgaacgcaag gcgctccgcg atgtggtggg cgagccgcgg ccacacccgg ccaccggccg
     9181 cttcatacgt gaggaggagc ttcgcgagcc cctcttcacc aaagaccata aggtgcgcgg
     9241 ccatgtcgat ggcagggtca tcaacgcggg cctcgctcca gtcgatcatc ccgctgacgc
     9301 gctccgtgtt gtcgatgagc acatggccca cgtagagatc gccatgcacc accacggaga
     9361 aatctggcca cgacgaatcg tcgtcgagcc agcgctgcca ccggtggagg cgcttgtcgt
     9421 tcaccacgaa ctcgcgtcgg acgcggtcaa cgtcgtcggc caccttctga cgggcctgcg
     9481 tcggtgtacg gatgagcatc cccgcatcca cggcggcgga aatggggacg gcatgcaggg
     9541 cggcgagcgc ggtcgcgaag ctctccgcga agacctccga gtcctgcggc acgacccagt
     9601 cgggcgtgga cgaaccaggc tggatgacca tcgcagtcga gtcttcgagc atgggatagg
     9661 caacgagctc ggcgttggcc acgcgccagt ccggcaccgc gaacggcagg cgattcttga
     9721 gcattgccag cacccgcgcc tctggttcga ccttcgcgct tacctcggct cggcgcggga
     9781 tgcgcagcac ccaccgacgt ccatcgtcga cggtggcgat cacgatccta tagtcgagcc
     9841 caagctcatt gacagtcagc gggccatgga gcttgagccc atgtcgggct gcaagtgcgt
     9901 acagttggga ggtatcggcg gtcgtgacta cggtcatgat tcactcctga gggcttgacg
     9961 ggtttagcca cctaaatgta acagtcacgt cggttatatt caatccggca ctgttgcaaa
    10021 gttagcgatg aggcagcctt ttgtct
//
LOCUS       DAHHTG010000415        10046 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.guided.149, whole genome shotgun sequence.
ACCESSION   DAHHTG010000415 DAHHTG010000000
VERSION     DAHHTG010000415.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10046)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10046)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10046
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.guided.149"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            complement(36..785)
                     /gene="intI1"
                     /locus_tag="NBI74_004824"
                     /pseudo
     CDS             complement(36..785)
                     /gene="intI1"
                     /locus_tag="NBI74_004824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221021.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 integron integrase IntI1"
     gene            943..1416
                     /gene="dfrA"
                     /locus_tag="NBI74_004825"
                     /pseudo
     CDS             943..1416
                     /gene="dfrA"
                     /locus_tag="NBI74_004825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001389366.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrA"
     gene            1547..2335
                     /locus_tag="NBI74_004826"
     CDS             1547..2335
                     /locus_tag="NBI74_004826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000503573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase AadA5"
                     /protein_id="HCD1935011.1"
                     /translation="MGEFFPAQVFKQLSHARAVIERHLAATLDTIHLFGSAIDGGLKP
                     DSDIDLLVTVSAAPNDSLRQALMLDLLKVSSPPGDGGTWRPLELTVVARSEVVPWRYP
                     ARRELQFGEWLRHDILSGTFEPAVLDHDLAILLTKARQHSLALLGPSAATFFEPVPKE
                     HFSKALFDTIAQWNAESDWKGDERNVVLALARIWYSASTGLIAPKDVAAAWVSERLPA
                     EHRPLICKARAAYLGSEDDDLAMRVEETAAFVRYAKATIERILR"
     gene            2541..2888
                     /locus_tag="NBI74_004827"
     CDS             2541..2888
                     /locus_tag="NBI74_004827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000679427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter QacE delta 1"
                     /protein_id="HCD1935012.1"
                     /translation="MKGWLFLVIAIVGEVIATSALKSSEGFTKLAPSAVVIIGYGIAF
                     YFLSLVLKSIPVGVAYAVWSGLGVVIITAIAWLLHGQKLDAWGFVGMGLIIAAFLLAR
                     SPSWKSLRRPTPW"
     gene            2882..3721
                     /gene="sul1"
                     /locus_tag="NBI74_004828"
     CDS             2882..3721
                     /gene="sul1"
                     /locus_tag="NBI74_004828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000259031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonamide-resistant dihydropteroate synthase
                     Sul1"
                     /protein_id="HCD1935013.1"
                     /translation="MVTVFGILNLTEDSFFDESRRLDPAGAVTAAIEMLRVGSDVVDV
                     GPAASHPDARPVSPADEIRRIAPLLDALSDQMHRVSIDSFQPETQRYALKRGVGYLND
                     IQGFPDPALYPDIAEADCRLVVMHSAQRDGIATRTGHLRPEDALDEIVRFFEARVSAL
                     RRSGVAADRLILDPGMGFFLSPAPETSLHVLSNLQKLKSALGLPLLVSVSRKSFLGAT
                     VGLPVKDLGPASLAAELHAIGNGADYVRTHAPGDLRSAITFSETLAKFRSRDARDRGL
                     DHA"
     gene            3849..4052
                     /locus_tag="NBI74_004829"
     CDS             3849..4052
                     /locus_tag="NBI74_004829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000376616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1935014.1"
                     /translation="MDSEEPPNVRVACSGDIDEVVRLMHDAAAWMSAKGTPAWDVARI
                     DRTFAETFVLRSELLGIASENGK"
     gene            4208..5413
                     /gene="chrA"
                     /locus_tag="NBI74_004830"
     CDS             4208..5413
                     /gene="chrA"
                     /locus_tag="NBI74_004830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004896675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromate efflux transporter"
                     /protein_id="HCD1935015.1"
                     /translation="MTNNPTDDSRPWSVFLIFLRLGLTSFGGPIAHLGYFRAEFVTRR
                     RWLSERSYADLVALCQFLPGPASSQVGIAVGLSRAGYSGALAAWAGFTLPSAIALILF
                     ALGISSYGDYVSQGALHGLKVVAVAVVAQAVWGMARNLCTDGLRVTIMAIATCVVLLV
                     PSAWGQVGVIAIAGIAGRLLFKPAKVVEHDPLPITVSHRAGVLWLSLFFVLLIGLPVL
                     AELMPSQTMAMVDSFYRVGSLVFGGGHVVLPLLQAEVVPSGWVNNESFLAGYGAAQAV
                     PGPLFTFAAFLGASMNTAPSGWIGGIVCLLAIFAPSFLLVVGSMPFWERLRRNTGIQA
                     ALAGINAAVVGLLLAALYQPVWTSAIFQPQDFGLALVALVALMFWKLPPWLVVLGSGA
                     AGWLLSVAL"
     gene            5424..5729
                     /locus_tag="NBI74_004831"
     CDS             5424..5729
                     /locus_tag="NBI74_004831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000130000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCD1935016.1"
                     /translation="MTDKDLYGGLIRLHILHHAAEEPVFGLGIIEELRRHGYEMSAGT
                     VYPMLHGLEKKGYLTSRHERTGRRERRVYDITEQGRTALADAKTKVKELFGELVEGG"
     gene            5773..5838
                     /locus_tag="NBI74_004832"
                     /pseudo
     CDS             5773..5838
                     /locus_tag="NBI74_004832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229646.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
     gene            5956..6720
                     /locus_tag="NBI74_004833"
     CDS             5956..6720
                     /locus_tag="NBI74_004833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001389365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS6100 family transposase"
                     /protein_id="HCD1935017.1"
                     /translation="MTDFKWRHFQGDVILWAVRWYCRYPISYRDLEEMLAERGISVDH
                     TTIYRWVQCYAPEMEKRLRWFWRRGFDPSWRLDETYVKVRGKWTYLYRAVDKRGDTID
                     FYLSPTRSAKAAKRFLGKALRGLKHWEKPATLNTDKAPSYGAAITELKREGKLDRETA
                     HRQVKYLNNVIEADHGKLKILIKPVRGFKSIPTAYATIKGFEVMRALRKGQARPWCLQ
                     PGIRGEVRLVERAFGIGPSALTEAMGMLNHHFAAAA"
     gene            complement(7213..7797)
                     /locus_tag="NBI74_004834"
     CDS             complement(7213..7797)
                     /locus_tag="NBI74_004834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001137892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCD1935018.1"
                     /translation="MPRPKLKSDDEVLEAATVVLKRCGPIEFTLSGVAKEVGLSRAAL
                     IQRFTNRDTLLVRMMERGVEQVRHYLNAIPIGAGPQGLWEFLQVLVRSMNTRNDFSVN
                     YLISWYELQVPELRTLAIQRNRAVVEGIRKRLPPGAPAAAELLLHSVIAGATMQWAVD
                     PDGELADHVLAQIAAILCLMFPEHDDFQLLQAHA"
     gene            complement(7797..9035)
                     /locus_tag="NBI74_004835"
     CDS             complement(7797..9035)
                     /locus_tag="NBI74_004835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCD1935019.1"
                     /translation="MSERRYSPLATLFAATFLFRIGNAVAALALPWFVLSHTKSAAWA
                     GATAASSVIATIIGAWVGGGLVDRFGRAPVALISGVVGGVAMASIPLLDAVGALSNTG
                     LIACVVLGAAFDAPGMAAQDSELPKLGHVAGLSVERVSSLKAVIGNVAILGGPALGGA
                     AIGLLGAAPTLGLTAFCSVLAGLLGAWVLPARAARTMTTTATLSMRAGVAFLWSEPLL
                     RPLFGIVMIFVGIVGANGSVIMPALFVDAGRQVAELGLFSSMMGAGGLLGIAIHASVG
                     ARISAQNWLAVAFCGSAVGSLLLSQLPGVPVLMLLGALVGLLTGSVSPILNAAIYNRT
                     PPELLGRVLGTVSAVMLSASPMVMLAAGAFVDLAGPLPGLVVSAVFAGLVALLSLRLQ
                     FATMAAAATASAPTHTEGEH"
     gene            complement(9032..9937)
                     /locus_tag="NBI74_004836"
     CDS             complement(9032..9937)
                     /locus_tag="NBI74_004836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000219391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mph(A) family macrolide 2'-phosphotransferase"
                     /protein_id="HCD1935020.1"
                     /translation="MTVVTTADTSQLYALAARHGLKLHGPLTVNELGLDYRIVIATVD
                     DGRRWVLRIPRRAEVSAKVEPEARVLAMLKNRLPFAVPDWRVANAELVAYPMLEDSTA
                     MVIQPGSSTPDWVVPQDSEVFAESFATALAALHAVPISAAVDAGMLIRTPTQARQKVA
                     DDVDRVRREFVVNDKRLHRWQRWLDDDSSWPDFSVVVHGDLYVGHVLIDNTERVSGMI
                     DWSEARVDDPAIDMAAHLMVFGEEGLAKLLLTYEAAGGRVWPRLAHHIAERLAFGAVT
                     YALFALDSGNEEYLAAAKAQLAAAE"
BASE COUNT         2091 a         2900 c         2956 g         2099 t
ORIGIN      
        1 gatgataagt ttatcaccac cgactatttg caacagtgcc gacgcacgac accgctccgt
       61 ggatcggtcg aatgcgtgtg ctgcgcaaaa acccagaacc acagccagga atgcccggcg
      121 cgcggatact tccgctcaag ggcgtcggga agcgcaacgc cgctgcggcc ctcggcctgg
      181 tccttcagcc accatgcccg tgcacgcgac agctgctcgc gcaggctggg tgccaagctc
      241 tcgggtaaca tcaaggcccg atccttggag cccttgccct cccgcacgat gatcgtgccg
      301 tgatcgaaat ccagatcctt gacccgcagt tgcaaaccct cactgatccg catgcccgtt
      361 ccatacagaa gctgggcgaa caaacgatgc tcgccttcca gaaaaccgag gatgcgaacc
      421 acttcatccg gggtcagcac caccggcaag cgccgcgacg gccgaggtct tccgatctcc
      481 tgaagccagg gcagatccgt gcacagcacc ttgccgtaga agaacagcaa ggccgccaat
      541 gcctgacgat gcgtggagac cgaaaccttg cgctcgttcg ccagccagga cagaaatgcc
      601 tcgacttcgc tgctgcccaa ggttgccggg tgacgcacac cgtggaaacg gatgaaggca
      661 cgaacccagt ggacataagc ctgttcggtt cgtaaactgt aatgcaagta gcgtatgcgc
      721 tcacgcaact ggtccagaac cttgaccgaa cgcagcggtg gtaacggcgc agtggcggtt
      781 ttcatggctt gttatgactg tttttttgta cagtctatgc ctcgggcatc caagcagcaa
      841 gcgcgttacg ccgtgggtcg atgtttgatg ttatggagca gcaacgatgt tacgcagcag
      901 ggcagtcgcc ctaaaacaaa gttagccatt aagggagtta aattgaaaat atcattgatt
      961 tctgcagtgt cagaaaatgg cgtaatcggt agtggtcctg atatcccgtg gtcagtaaaa
     1021 ggtgagcaac tactctttta agcgctcaca tataatcaat ggctccttgt cggaagaaaa
     1081 acatttgact ctatgggtgt tcttccaaat cgcaaatatg cagtagtgtc aaagaacgga
     1141 atttcaagct caaatgaaaa cgtcctagtt tttccttcaa tagaaaatgc tttgaaagag
     1201 ctatcaaaag ttacagatca tgtatatgtc tctggcgggg gtcaaatcta taatagcctt
     1261 attgaaaaag cagatataat tcatttgtct actgttcacg ttgaagtcga aggtgatatc
     1321 aaattcccta taatgcctga gaatttcaat ttggtttttg aacagttttt tatgtctaat
     1381 ataaattata cataccagat ttggaaaaaa ggctaacaat gcgttgcagc accagtcgct
     1441 tcgctccttg gacagctttt aagtcgcgtc tttgtggttt tgctgcgcaa aagtattcca
     1501 caaagccgca acttaaaagc tgccgctgaa cttaacgtta ggcatcatgg gtgaattttt
     1561 ccctgcacaa gttttcaagc agctgtccca cgctcgcgcg gtgatcgagc gccatctggc
     1621 tgcgacactg gacacaatcc acctgttcgg atctgcgatc gatggagggc tgaagccgga
     1681 cagcgacata gacttgctcg tgaccgtcag cgccgcacct aacgattcgc tccggcaggc
     1741 gctaatgctc gatttgctga aagtctcatc accgccaggc gatggcggaa catggcgacc
     1801 gctggagcta actgttgtcg ctcgaagcga agtagtgcct tggcgctatc cggcgcggcg
     1861 tgagcttcag ttcggtgagt ggctccgcca cgacatcctt tccggaacgt tcgagcctgc
     1921 cgttctggat cacgatcttg cgattttgct gaccaaggcg aggcaacaca gccttgcgct
     1981 tctaggccca tccgcagcca cgtttttcga gccggtgccg aaggagcatt tctccaaggc
     2041 gcttttcgac actattgccc agtggaatgc agagtcggat tggaagggtg acgagcggaa
     2101 cgtcgttctt gctcttgctc gcatttggta cagcgcttca actggtctca ttgctcctaa
     2161 ggacgttgct gccgcatggg tatcggagcg tttgcctgcc gagcatcggc ccctcatctg
     2221 caaggcacgc gcggcgtacc tgggtagcga ggacgacgac ctagcaatgc gcgtcgaaga
     2281 gacggccgcg ttcgttcgat atgccaaagc aacgattgag agaatcttgc gttgagcggc
     2341 atgtgcgaaa agtgcatcga cccgcgccga gggcatctga tgcctaactc ggcgttcaag
     2401 cggacgggct gcgcccgccg ctcaactatg cgttagatgc actaagcaca taattgctca
     2461 cagccaaact atcaggtcaa gtctgctttt attattttta agcgtgcata ataagcccta
     2521 cacaaattgg gagatatatc atgaaaggct ggctttttct tgttatcgca atagttggcg
     2581 aagtaatcgc aacatccgca ttaaaatcta gcgagggctt tactaagctt gccccttccg
     2641 ccgttgtcat aatcggttat ggcatcgcat tttattttct ttctctggtt ctgaaatcca
     2701 tccctgtcgg tgttgcttat gcagtctggt cgggactcgg cgtcgtcata attacagcca
     2761 ttgcctggtt gcttcatggg caaaagcttg atgcgtgggg ctttgtaggt atggggctca
     2821 taattgctgc ctttttgctc gcccgatccc catcgtggaa gtcgctgcgg aggccgacgc
     2881 catggtgacg gtgttcggca ttctgaatct caccgaggac tccttcttcg atgagagccg
     2941 gcggctagac cccgccggcg ctgtcaccgc ggcgatcgaa atgctgcgag tcggatcaga
     3001 cgtcgtggat gtcggaccgg ccgccagcca tccggacgcg aggcctgtat cgccggccga
     3061 tgagatcaga cgtattgcgc cgctcttaga cgccctgtcc gatcagatgc accgtgtttc
     3121 aatcgacagc ttccaaccgg aaacccagcg ctatgcgctc aagcgcggcg tgggctacct
     3181 gaacgatatc caaggatttc ctgaccctgc gctctatccc gatattgctg aggcggactg
     3241 caggctggtg gttatgcact cagcgcagcg ggatggcatc gccacccgca ccggtcacct
     3301 tcgacccgaa gacgcgctcg acgagattgt gcggttcttc gaggcgcggg tttccgcctt
     3361 gcgacggagc ggggtcgctg ccgaccggct catcctcgat ccggggatgg gatttttctt
     3421 gagccccgca ccggaaacat cgctgcacgt gctgtcgaac cttcaaaagc tgaagtcggc
     3481 gttggggctt ccgctattgg tctcggtgtc gcggaaatcc ttcttgggcg ccaccgttgg
     3541 ccttcctgta aaggatctgg gtccagcgag ccttgcggcg gaacttcacg cgatcggcaa
     3601 tggcgctgac tacgtccgca cccacgcgcc tggagatctg cgaagcgcaa tcaccttctc
     3661 ggaaaccctc gcgaaatttc gcagtcgcga cgccagagac cgagggttag atcatgccta
     3721 gcattcacct tccggccgcc cgctagcgga ccctggtcag gttccgcgaa ggtgggcgca
     3781 gacatgctgg gctcgtcagg atcaaactgc actatgaggc ggcggttcat accgcgccag
     3841 gggagcgaat ggacagcgag gagcctccga acgttcgggt cgcctgctcg ggtgatatcg
     3901 acgaggttgt gcggctgatg cacgacgctg cggcgtggat gtccgccaag ggaacgcccg
     3961 cctgggacgt cgcgcggatc gaccggacat tcgcggagac cttcgtcctg agatccgagc
     4021 tcctagggat cgcctcagaa aacggaaaat aaagcacgct aagccgtaag taagcgtgct
     4081 cctgtgaaag ccacagctaa aactgcgtag tacacataga ggtatttttt tctagattta
     4141 tatcgaattt cgttatcgaa tttcgcttat tattgatcaa ctcgttgcag atcatgaagg
     4201 cgaaagtatg acgaataacc ctaccgatga cagcagacca tggtcggtct ttcttatttt
     4261 tctgcggctt ggattgacat cttttggcgg ccccattgcg cacttgggct acttccgcgc
     4321 cgaatttgtc acacggcggc gctggctctc cgaacggagc tatgctgact tggtcgcgct
     4381 ttgtcagttc ttgccagggc ctgcaagcag ccaggtcggc atagcggtag gactgtctcg
     4441 ggctggatac agcggggcgc tggctgcttg ggctggcttc acgctgccgt ctgccatagc
     4501 cttgatcctt tttgcgctcg gcatctccag ctatggcgat tacgtctcgc agggcgcgtt
     4561 gcatggctta aaagtggtgg ctgtggccgt ggtcgctcaa gcagtatggg gcatggcgcg
     4621 taacctatgc acggatgggc tgcgagtcac catcatggca attgctacct gcgtcgtttt
     4681 acttgtgccg tccgcgtggg gacaggttgg cgtgattgct atcgcaggca tcgcaggccg
     4741 gttattgttc aagccagcga aagttgttga gcatgacccc ctacctatca cggtcagtca
     4801 ccgggccggc gtgctttggc tctcgctgtt ctttgtcttg ctgattggcc tgccggtgtt
     4861 ggccgaactg atgccaagtc aaaccatggc aatggtggat tccttctatc gtgtcggatc
     4921 actggtgttc ggcggtggtc acgttgtgct gccattactg caagccgaag tggtgccctc
     4981 cggctgggtc aacaatgaat cctttctcgc ggggtacggg gcagctcaag cggtgcccgg
     5041 ccctttgttc acgttcgccg cgtttcttgg tgcctcgatg aacaccgccc cgtcgggctg
     5101 gatcggcggc attgtgtgtc tgctggctat cttcgcgccc tcgttcttgc tggtcgtcgg
     5161 atcaatgcca ttttgggagc gtttgcgccg caatacaggc atccaagctg cgctggccgg
     5221 gatcaatgcc gctgtagtcg gcttgctgct ggccgcgctg tatcagcctg tatggactag
     5281 cgccatcttt cagccgcaag acttcggctt ggcattagtt gcccttgtcg cacttatgtt
     5341 ctggaagctc ccgccgtggc tggttgtcct cggtagcggt gcagctgggt ggctgttgag
     5401 cgtcgctctg tgaggcacaa aaaatgactg acaaagacct ctacggcggt ttgatccgcc
     5461 tgcacatcct tcaccatgca gccgaggaac ctgtctttgg gctggggatc atcgaagagc
     5521 tacgccgaca cggctacgag atgagcgctg gcaccgtgta cccgatgctg cacggcctgg
     5581 aaaaaaaagg ctatctgacc tcacgccacg aacgcaccgg gcgacgtgaa cggcgtgttt
     5641 atgacatcac tgaacaaggc agaactgcac ttgctgatgc gaaaacaaag gtcaaggagc
     5701 tgttcggaga gttggtagaa ggtggttgag tttttgcttc tatgtcaggt tgagctatac
     5761 cctaactgga ttgtcatttt cagaagacga ctgcaccagt tgattgggcg taatggctgt
     5821 tgtgcagcca gctcctgaca gttcaatatc agaagtgatc tgcaccaatc tcgactatgc
     5881 tcaatactcg tgtgggctct gttgcaaaaa tcgtgaagct tgagcatgct tggcggagat
     5941 tggacggacg gaacgatgac ggatttcaag tggcgccatt tccagggtga tgtgatcctg
     6001 tgggcggtgc gctggtattg tcgctatccg atcagctatc gcgaccttga ggaaatgctg
     6061 gcggaacgcg gcatttcggt cgaccatacg acgatctatc gctgggtcca gtgctacgcc
     6121 ccggagatgg agaagcggct gcgctggttc tggcggcgtg gctttgatcc gagctggcgc
     6181 ctggatgaaa cctacgtcaa ggtgcggggc aagtggacct acctgtaccg ggcagtcgac
     6241 aagcggggcg acacgatcga tttctacctg tcgccgaccc gcagcgccaa ggcagcgaag
     6301 cggttcctgg gcaaggccct gcgaggcctg aagcactggg aaaagcctgc cacgctcaat
     6361 accgacaaag cgccgagcta tggtgcagcg atcaccgaat tgaagcgcga aggaaagctg
     6421 gaccgggaga cggcccaccg gcaggtgaag tatctcaata acgtgatcga ggccgatcac
     6481 ggaaagctca agatactgat caagccggtg cgcggtttca aatcgatccc cacggcctat
     6541 gccacgatca agggattcga agtcatgcga gccctgcgca aaggacaggc tcgcccctgg
     6601 tgcctgcagc ccggcatcag gggcgaggtg cgccttgtgg agagagcttt tggcattggg
     6661 ccctcggcgc tgacggaggc catgggcatg ctcaaccacc atttcgcagc agccgcctga
     6721 tcggcgcaga gcgacagcct acctctgact gccgccaatc tttgcaacag agcccgccgt
     6781 gctagtctgc tcggtgatgg tggagtgaag ccaacccgca atcgggttat gaatctgcat
     6841 cgcgattcgc aatcagctgt ctcttgagca tgtcgaactc ctgcgatgtt atctcgccgt
     6901 gctcgtgctg ctacaccaac ttgtccaact catccgccac accgctcccc accacctgcg
     6961 atggccaggt tgtttgctgg gcgcattggc ggagtatcgt ctcgacccgg gacacccaca
     7021 ccggcacaac cttacgggag tgattcactg tcaaagaatc ggcccggtgc tctgacgcaa
     7081 gtatcgggat ggtcaccatt tgtaagccgt agacctgagt ggtgatcaag acttcgatac
     7141 caccgaccgt accggtacta atcgacgacg gtcgtgttcg tcgcctgccg cagggactct
     7201 gcacacctcc gtttacgcat gtgcctggag gagttggaaa tcgtcgtgtt cgggaaacat
     7261 taaacacagg atggcagcga tctgagccag cacatgatca gctagctcac catccggatc
     7321 gacggcccac tgcatcgtcg cgccagcgat gaccgagtgc aggagcaact cagctgccgc
     7381 aggagcacct gggggcagtc gcttgcggat cccctccacc accgcgcggt tccgctggat
     7441 cgcaagcgtg cgtagctccg gcacctggag ctcgtaccag gagatgagat agttcaccga
     7501 gaagtcgttg cgagtgttca tgctccgaac gagcacctgc aaaaattccc agagcccttg
     7561 cggccctgcg cctatcggta tcgcattcag gtaatgccgc acctgctcga cgccgcgctc
     7621 catcatcctc accagcagcg tatcgcggtt ggtgaagcgc tggattaacg ctgcgcggga
     7681 gagccccacc tcctttgcta ctccgctgag cgtgaactct atgggaccgc aacgcttcag
     7741 cactacggtg gcggcctcga gtacctcgtc atcggacttg agcttggggc ggggcatcag
     7801 tgttcacctt ctgtatgggt tggggcggag gctgtggctg ccgccgccat tgtagcaaat
     7861 tgaagacgga gcgagagtag agccacgagc cccgcaaaca cggccgatac aacgaggcca
     7921 gggagcggac cagcaaggtc gacaaacgcg ccggccgcaa gcataaccat gggcgaggct
     7981 gacagcatca ccgccgagac cgtgccgagt acccggccga gaagttctgg cggcgtgcgg
     8041 ttgtagatgg cagcgttgag aatgggagag actgagccgg tcagcagtcc cacgagcgcg
     8101 cccaacaaca tcagcaccgg cacgcctggc aactgtgaaa gcagaagcga gcccaccgca
     8161 gagccacaaa atgccaccgc cagccagttc tgcgctgata tccgggcgcc gaccgacgca
     8221 tgaatggcaa tgccaaggag accaccagcc cccatcattg aggagaacag cccgagctct
     8281 gctacttggc gtcctgcatc tacaaacagc gcaggcatga tgacgctgcc gttggcgcca
     8341 acgatgccca cgaagatcat cactatacca aagagagggc gcagcagggg ttcgctccag
     8401 agaaaagcga cgccggcgcg catggagaga gtcgccgtcg tggtcatcgt ccgagcggca
     8461 cgcgcgggaa gcacccacgc gccgagcaga cctgcaagga cggagcagaa cgccgtcagc
     8521 ccgagcgttg gcgcagcgcc aagcaggccg attgcggccc ccccaagggc cgggccacct
     8581 agaatcgcga cgttcccgat caccgctttc agtgacgaga cgcgctcaac ggagagcccg
     8641 gcgacgtggc cgagtttggg cagctcactg tcctgcgcgg ccataccggg tgcgtcgaac
     8701 gcggcaccga gcaccacgca agcgatcagc ccagtgttcg agagggcgcc aacggcatcg
     8761 agcagtggga tgctcgccat ggccacgccg cccaccacac ccgagatcaa tgcgacgggc
     8821 gcgcgcccga accgatcgac gaggccacca ccaacccacg cgccgatgat ggtcgcgatg
     8881 acgctgctag cggccgtggc gcccgcccag gccgcgctct ttgtatgaga caggacgaac
     8941 catggaagcg cgagggccgc caccgcgttg ccgatccgga agagaaaggt cgccgcgaac
     9001 agcgtcgcga gcgggctata tcgacgttcg ctcattccgc tgcggcgagc tgcgccttcg
     9061 ccgcagcgag gtactcttcg ttacccgagt cgagggcgaa gagtgcgtag gtgaccgccc
     9121 cgaacgcaag gcgctccgcg atgtggtggg cgagccgcgg ccacacccgg ccaccggccg
     9181 cttcatacgt gaggaggagc ttcgcgagcc cctcttcacc aaagaccata aggtgcgcgg
     9241 ccatgtcgat ggcagggtca tcaacgcggg cctcgctcca gtcgatcatc ccgctgacgc
     9301 gctccgtgtt gtcgatgagc acatggccca cgtagagatc gccatgcacc accacggaga
     9361 aatctggcca cgacgaatcg tcgtcgagcc agcgctgcca ccggtggagg cgcttgtcgt
     9421 tcaccacgaa ctcgcgtcgg acgcggtcaa cgtcgtcggc caccttctga cgggcctgcg
     9481 tcggtgtacg gatgagcatc cccgcatcca cggcggcgga aatggggacg gcatgcaggg
     9541 cggcgagcgc ggtcgcgaag ctctccgcga agacctccga gtcctgcggc acgacccagt
     9601 cgggcgtgga cgaaccaggc tggatgacca tcgcagtcga gtcttcgagc atgggatagg
     9661 caacgagctc ggcgttggcc acgcgccagt ccggcaccgc gaacggcagg cgattcttga
     9721 gcattgccag cacccgcgcc tctggttcga ccttcgcgct tacctcggct cggcgcggga
     9781 tgcgcagcac ccaccgacgt ccatcgtcga cggtggcgat cacgatccta tagtcgagcc
     9841 caagctcatt gacagtcagc gggccatgga gcttgagccc atgtcgggct gcaagtgcgt
     9901 acagttggga ggtatcggcg gtcgtgacta cggtcatgat tcactcctga gggcttgacg
     9961 ggtttagcca cctaaatgta acagtcacgt cggttatatt caatccggca ctgttgcaaa
    10021 gttagcgatg aggcagcctt ttgtct
//
LOCUS       DAHHTG010000416         6071 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.guided.200, whole genome shotgun sequence.
ACCESSION   DAHHTG010000416 DAHHTG010000000
VERSION     DAHHTG010000416.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6071)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6071)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6071
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.guided.200"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            <1..1002
                     /locus_tag="NBI74_004837"
     CDS             <1..1002
                     /locus_tag="NBI74_004837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000521550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
                     /protein_id="HCD1935021.1"
                     /translation="DLHPGRGYPIGAAFFSVGRFYPALVGNDIGCGMALWQTDILARK
                     YNADKFEKRLSDLDDVAEESWLEENLPSAFAQHPWHNSLGSIGGGNHFAELQQIDQII
                     DAELFARAGLDAQHLQLLVHSGSRGLGQSILQRHIASFSHHGLPEGSDDALRYIAEHD
                     DALAFARINRQLIALRIMQQVKATGSPVLDVAHNFVSACQIGDQQGWLHRKGATPDDN
                     GLVIIPGSRGDYSWLVKPVANEKTLHSLAHGAGRKWGRTECKGRLAAKYTATQLSRTE
                     LGSRVICRDKQLIFEEAPQAYKSAESVVQCLVLAGLIIPVARLRPVLTLKNSGGKKG"
     gene            999..1613
                     /locus_tag="NBI74_004838"
     CDS             999..1613
                     /locus_tag="NBI74_004838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor H"
                     /protein_id="HCD1935022.1"
                     /translation="MILLQLSSAQGPEECCLAVKKALDRLIKEATRQDVAVTVLETET
                     GRYSDTLRSALISLDGDNAWALSESWCGTIQWICPSPYRPHHGRKNWFLGIGRFTADE
                     QEQSDAIRYETLRSSGPGGQHVNKTDSAVRATHLASGISVKVQSERSQHANKRLARLL
                     IAWKLEQQQQENSAALKSQRRMFHHQIERGNPRRTFTGMAFIEG"
     gene            complement(1669..3126)
                     /gene="pepD"
                     /locus_tag="NBI74_004839"
     CDS             complement(1669..3126)
                     /gene="pepD"
                     /locus_tag="NBI74_004839"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HCD1935023.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRKPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFNLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDAL
                     KSLVDTYQEILKNELAEKEKNLALLLDSVANDKAALTATSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPAK"
     gene            3387..3845
                     /gene="gpt"
                     /locus_tag="NBI74_004840"
     CDS             3387..3845
                     /gene="gpt"
                     /locus_tag="NBI74_004840"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HCD1935024.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQNTWIEQPWDMGVVFVPPISGR"
     gene            3937..5181
                     /gene="frsA"
                     /locus_tag="NBI74_004841"
     CDS             3937..5181
                     /gene="frsA"
                     /locus_tag="NBI74_004841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HCD1935025.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFSHGAQLPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILEVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACAEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     ALRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDEA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITDWIEKRLC"
     gene            5239..5640
                     /gene="crl"
                     /locus_tag="NBI74_004842"
     CDS             5239..5640
                     /gene="crl"
                     /locus_tag="NBI74_004842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HCD1935026.1"
                     /translation="MTLPSGHPKSRLVKKFTALGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWTSVQIKDAEVVERLEHTLRE
                     FHEKLRELLATLNLKLEPADDFRDEPVKLTA"
     gene            complement(5679..>6071)
                     /locus_tag="NBI74_004843"
     CDS             complement(5679..>6071)
                     /locus_tag="NBI74_004843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HCD1935027.1"
                     /translation="RGTGKRAEAWATGLKYDANNIYLATFYSETRKMTPISGGFANKT
                     QNFEAVAQYQFDFGLRPSLGYVLSKGKDIEGIGDEDLVNYVDVGATYYFNKNMSAFVD
                     YKINQLDSDNKLNINNDDIVAVGMTYQF"
BASE COUNT         1469 a         1491 c         1613 g         1498 t
ORIGIN      
        1 gatttacatc ccggacgcgg ctacccgatt ggcgcagcgt tcttctctgt tggtcgtttt
       61 tacccggcac tggtcggcaa tgatatcggc tgcggcatgg cgctatggca aacagatatt
      121 ctcgctcgca aatacaacgc cgataagttt gaaaagcgat tatcagatct ggatgacgtt
      181 gctgaagaaa gctggctgga ggaaaacctg ccgtcagcat ttgcacagca tccgtggcac
      241 aactcgcttg gctccatcgg tggcggtaac cacttcgcag aactgcaaca gattgatcaa
      301 attatcgacg cggaactgtt tgcacgggct ggtctggatg cgcagcatct gcaactactg
      361 gttcatagcg gctcgagggg attggggcag tctattttac agcggcatat tgcctcgttt
      421 tcgcatcatg gtttgcctga aggcagtgac gacgcgctac ggtatattgc ggaacatgac
      481 gatgcgctgg cgtttgcgcg tattaatcgc cagctgatcg ctttgcgcat aatgcaacaa
      541 gttaaggcca ccggaagtcc ggttctggat gtggcgcata actttgttag cgcgtgccaa
      601 atcggcgatc aacagggctg gttgcatcgt aaaggcgcca cgccggatga caacggtctg
      661 gtgattattc ccggttcacg cggcgattac tcctggctgg ttaaacctgt tgcgaacgaa
      721 aaaacgctcc actcgctggc gcatggggcg gggcgtaaat gggggcgcac cgagtgtaaa
      781 gggcgtctgg cagcgaaata cacggcgacg caactctcgc ggactgaact tggcagtcgg
      841 gtaatttgtc gcgataaaca actcatcttt gaagaagcgc cacaagccta taaatcggct
      901 gaaagcgtgg tgcaatgtct ggtgctggct gggttaatta ttcctgtcgc gcgactgcgc
      961 ccggtgctga cgctcaaaaa cagtggaggg aaaaaaggat gatcttgcta caactctcct
     1021 ctgctcaggg gccggaagaa tgttgtctcg cagtgaaaaa agcactggac aggctgatta
     1081 aagaagctac ccgacaggac gtcgcggtaa cggtgctgga aacagaaacg ggtcgctact
     1141 ctgacacact gcgttcggcg ctgatttctc tggatggcga taacgcatgg gctctaagcg
     1201 aaagctggtg cggcactatt cagtggattt gtccgagtcc gtatcggcct catcatgggc
     1261 gcaaaaactg gtttctgggc attgggcgtt ttaccgctga tgagcaggaa caatcggatg
     1321 caatccgtta tgagacgctg cgttcgtcgg ggccgggcgg tcaacatgtc aataaaaccg
     1381 actcggcggt acgcgccacg catctggcat ccggtattag cgtgaaggtt cagtcagagc
     1441 gcagtcagca tgctaacaaa cggctggcgc gattactgat tgcctggaag ctggaacaac
     1501 agcaacagga aaatagcgcg gcgctgaaat cgcagcggcg aatgttccat caccagattg
     1561 aacgtggcaa cccgcgacga acgttcacag ggatggcttt tatcgaagga taatgaagga
     1621 tgaaactgcc ggaaggcgtt aaacgccatc cggcagcaaa tcaaataatt acttcgccgg
     1681 aatttctttc agcagttcag tcagcagtgt ccagtaatgg ccaacgcttt cgatatgaac
     1741 ttgctcatcc ggggagtgtg gaccggtgat ggttggcccg atagacacca tgtccatttc
     1801 cggatacggt tttttgaaca gaccacattc caggcccgcg tggataatct ggatgttcgg
     1861 cgtcttgttg aacagacgct gataggtttc acgtaccaga tgcattaccg gagagttagc
     1921 gtccggctgc cagccaggat atgcgccttt tgcttcggtt ttcgcacctg ccagtttacc
     1981 cagcgaatcc agcatgctca ctacgtagtc tttaccgctg tcgatcagag aacggatcag
     2041 gcagtgaatt tctacgttat tgtcggtcat ggtcaccaca ccgacgttca gggaggtttc
     2101 aaccacgcct ttggctacat cggaattacg gataactccg ttcggggtgg cgttcagcag
     2161 acgaataaag gtatcgcgag atgtcgccgt taatgcagct ttgtcgttcg ctacggagtc
     2221 cagcagcaat gccagattct tctctttttc tgccagttcg tttttcagga tctcctgata
     2281 ggtatccacc agagatttca gggcgtcgac tttatcagct gcgacagcaa tggtcgcaaa
     2341 ggcttcacgc gggatggcgt tacgcagtgt gccgccgttg aaatcaataa gacgcaggtc
     2401 cagttcttct gcatgacccg ccaggaagcg caccagcagt ttgttggcat tacccaggcc
     2461 aacgtggatt tcaccgccgg agtgaccgcc tttcagacct tttaaggtta aattgaaggt
     2521 ttcaaaacca gctggaaccg cttcacgatc taaatgcagg ttggaggtga agtcgatacc
     2581 ccccgcacat cccatgtaga tttcaccttc ttcttcggag tcggtgttaa tcagaatatc
     2641 agcctgcaac cagttgctct gtaagccgaa cgcaccgtcc ataccggctt cttcagtcat
     2701 ggtcagcagc acttccagcg ggccgtgaac cacgttttcg tcagccagaa ccgccagtgc
     2761 ggaggccata ccaatgccgt tatccgcacc cagcgtggtg ccgcgcgctt taacccattc
     2821 gccatcaata taaggctgga taggatcttt agtgaagtca tgcacggtgt cgttattttt
     2881 ttgcggcacc atatcgaggt gggcctgcaa gacgaccggt ttacgatttt ccatacctgc
     2941 ggtagcaggt ttacgaatca ggatattacc tacctgatcg cgttcgacat ggaaaccttt
     3001 ctcttttgcc caaccaacaa tgtattcagc gagttgctct tcatgatagg acgggtgagg
     3061 aatagaacag attttggcaa aaatatccca cagcggctgt ggagataatt gagacagttc
     3121 agacacgtta agtctccttg tcgatcaccc gcaaaacagt attgcaggtc acagggttag
     3181 cagaaaatgt tgtcaacaca agacaggctt gcgagatatg tttgagaata ccactttatc
     3241 ccgcgtcagg gagaggcagt gcgtaaaaag acgcggactc atgtgaaata ctggttttta
     3301 gtgcgccaga tctctataat ctcgcgcaac ctattttccc ctcgaacact ttttaagccg
     3361 tagataaaca ggctgggaca cttcacatga gcgaaaaata catcgtcacc tgggacatgt
     3421 tgcagatcca tgcacgtaaa ctcgcaagcc gactgatgcc ttctgaacaa tggaaaggca
     3481 ttattgccgt aagccgtggc ggtctggtac cgggtgcgtt actggcgcgt gaactgggta
     3541 ttcgtcatgt cgataccgtt tgtatttcca gctacgatca cgacaaccag cgcgagctta
     3601 aagtgctgaa acgcgcagaa ggcgatggcg aaggcttcat cgttattgat gacctggtag
     3661 ataccggtgg tactgcggtt gcgattcgtg aaatgtatcc aaaagcgcac tttgtcacca
     3721 tcttcgcaaa accggctggt cgtccgctgg ttgatgatta tgttgttgat atcccgcaaa
     3781 atacctggat cgaacagccg tgggatatgg gcgtcgtatt cgtcccgcca atctccggtc
     3841 gctaatcttt tcaacgcctg gcactgccgg gcgttgttct ttttaacttc aggcgggtta
     3901 caatagtttc cagtaagtat tctggaggct gcatctatga cacaggcaaa cctgagcgaa
     3961 accctgttca aaccccgctt taaacatcct gaaacctcga cgctagtccg ccgctttagt
     4021 cacggcgcac aactgcctgt gcagtcggcc cttgatggta aaaccatccc tcactggtat
     4081 cgcatgatta accgtctgat gtggatctgg cgcggcattg acccccgtga aatcctcgaa
     4141 gtccaggcgc gtattgtgat gagcgatgcc gaacgtaccg acgatgattt atacgatacg
     4201 gtgattggct accgcggcgg caactggatt tatgagtggg ctacccaggc gatggtgtgg
     4261 caacaaaaag cctgtgcgga agaagatccg caactcagcg gtcgacactg gctgcatgcg
     4321 gctacgttgt acaacattgc tgcctatcct catctgaaag gagatgacct ggccgagcaa
     4381 gcgcaggctt tgtcaaaccg cgcctatgaa gaggccgctc agcgtctgcc gggcacgatg
     4441 cggcagatgg agtttaccgt acccggcggt gcgcccatca ccggcttttt gcatatgccg
     4501 aaaggcgatg gcccgttccc gacagtatta atgtgtggtg gtctggatgc gatgcagaca
     4561 gactattaca gcctgtatga acgttatttt gcgctgcgcg gcattgcgat gctgactatt
     4621 gatatgccgt cggtgggctt ttcttcaaaa tggaagctca cccaggactc cagcctgttg
     4681 catcagcacg ttttaaaggc gttgcctaac gtaccgtggg tggatcacac tcgcgtcgcg
     4741 gcctttggtt tccgtttcgg cgctaacgtt gccgtacgtc tggcatacct tgaatcgccg
     4801 cgtctgaaag cggttgcctg tcttggtccg gtagttcata ccctgttgag tgattttaag
     4861 tgccagcaac aggtgccgga aatgtatctt gacgttctgg cgagtcgttt ggggatgcat
     4921 gatgcttctg acgaagcgtt gcgcgtggag ctgaatcgct attcattaaa agtgcaaggg
     4981 ttgctgggac gtcgctgccc aacgccaatg ttatcaggct actggaagaa cgatccgttc
     5041 agcccggaag aggactcacg cttaatcacg tcgtcatctg ctgacggtaa attattagag
     5101 atcccattta acccggtgta tcggaatttt gacaaaggcc ttcaggaaat cacagactgg
     5161 atcgaaaaac gtttgtgtta aaaatttgct aaattttacc aatttggtaa aacagttgca
     5221 tcacaacagg agattgcaat gacgttaccg agtggacacc cgaagagcag attagtcaaa
     5281 aaatttaccg cactaggccc gtatattcgt gaaggtaagt gcgaagataa tcgattcttt
     5341 ttcgattgtc tggctgtatg cgtcaacgtg aaaccggcac cggaagtgcg tgagttctgg
     5401 ggctggtgga tggaactgga agcgcaggaa tcccgtttta cctacagtta ccagtttggt
     5461 ctgttcgata aagcaggcga ctggacgagt gttcagataa aagatgccga agtggttgaa
     5521 cgactggagc acaccctgcg tgaatttcac gagaagctgc gtgagctgct ggcgacgctg
     5581 aatctgaagc tggaaccggc agatgatttt cgtgatgaac cggtgaagtt aacggcgtga
     5641 atgaaatgtg ccggatgcat cacatccggc aatatccatt aaaactgata ggtcatgcca
     5701 accgcgacaa tatcatcatt attaatattc aatttgttat cgctatccag ttggttgatt
     5761 ttataatcaa caaacgctga catatttttg ttgaaataat acgtagcacc aacatcgaca
     5821 taattgacca gatcttcatc accgatgccc tcaatatctt tccctttcga taagacataa
     5881 cccagcgatg gacgcagacc aaagtcaaac tggtattgag cgaccgcttc aaagttctgc
     5941 gtcttattgg caaagccgcc agagattggc gtcattttac gtgtttcaga atagaaagtt
     6001 gccagataaa tattattggc atcgtatttc agacctgttg cccatgcttc tgcacgcttg
     6061 cctgtgccac g
//
LOCUS       DAHHTG010000417         3680 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.guided.244, whole genome shotgun sequence.
ACCESSION   DAHHTG010000417 DAHHTG010000000
VERSION     DAHHTG010000417.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3680)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3680)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3680
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.guided.244"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            238..1113
                     /locus_tag="NBI74_004844"
     CDS             238..1113
                     /locus_tag="NBI74_004844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032492044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A extended-spectrum beta-lactamase
                     CTX-M-27"
                     /protein_id="HCD1935028.1"
                     /translation="MVTKRVQRMMFAAAACIPLLLGSAPLYAQTSAVQQKLAALEKSS
                     GGRLGVALIDTADNTQVLYRGDERFPMCSTSKVMAAAAVLKQSETQKQLLNQPVEIKP
                     ADLVNYNPIAEKHVNGTMTLAELSAAALQYSDNTAMNKLIAQLGGPGGVTAFARAIGD
                     ETFRLDRTEPTLNTAIPGDPRDTTTPRAMAQTLRQLTLGHALGETQRAQLVTWLKGNT
                     TGAASIRAGLPTSWTVGDKTGSGGYGTTNDIAVIWPQGRAPLVLVTYFTQPQQNAESR
                     RDVLASAARIIAEGL"
     gene            1148..2116
                     /locus_tag="NBI74_004845"
     CDS             1148..2116
                     /locus_tag="NBI74_004845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5-like element IS903B family transposase"
                     /protein_id="HCD1935029.1"
                     /translation="MKDQITYLPDNADRSVAKQKFKITNWPTYNKALINRGSITFWLD
                     DEAIQAWYESATPSSRGRPQRYSDLAITTVLVIKRVFRLTLRAAQGFIDSIFSLMNVP
                     LRCPDYSCVSRRAKSVNISFKTPTRGEIAHLVIDSTGLKVFGEGEWKVKKHGQERRRI
                     WRKLHLAVDSKTHEIICADLSLNNVTDSEAFPGLIRQTHRKIRSAAADGAYDTRLCHD
                     ELRRKKISALIPPRKGAGYWPGEYADRNRAVANQRMTGSNARWKWTTDYNRRSIAETA
                     MYRVKQLFGGSLTLRDYDGQVAEAMALVRALNKMTKAGMPESVRIA"
     gene            2173..3270
                     /locus_tag="NBI74_004846"
                     /pseudo
     CDS             2173..3270
                     /locus_tag="NBI74_004846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965681.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
     gene            complement(3338..3610)
                     /locus_tag="NBI74_004847"
                     /pseudo
     CDS             complement(3338..3610)
                     /locus_tag="NBI74_004847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_073984355.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4158 domain-containing protein"
BASE COUNT          912 a          927 c         1058 g          783 t
ORIGIN      
        1 caataaaatt gagtgttgct ctgtggataa cttgcagagt ttattaagta tcattgcagc
       61 aaagatgaaa tcaatgattt atcaaaaatg attgaaaggt ggttgtaaat aatgttacaa
      121 tgtgtgagaa gcagtctaaa ttcttcgtga aatagtgatt tttgaagcta ataaaaaaca
      181 cacgtggaat ttagggaata ctgatgtaac acggattgac cgtattggga gtttgagatg
      241 gtgacaaaga gagtgcaacg gatgatgttc gcggcggcgg cgtgcattcc gctgctgctg
      301 ggcagcgcgc cgctttatgc gcagacgagt gcggtgcagc aaaagctggc ggcgctggag
      361 aaaagcagcg gagggcggct gggcgtcgcg ctcatcgata ccgcagataa tacgcaggtg
      421 ctttatcgcg gtgatgaacg ctttccaatg tgcagtacca gtaaagttat ggcggccgcg
      481 gcggtgctta agcagagtga aacgcaaaag cagctgctta atcagcctgt cgagatcaag
      541 cctgccgatc tggttaacta caatccgatt gccgaaaaac acgtcaacgg cacaatgacg
      601 ctggcagaac tgagcgcggc cgcgttgcag tacagcgaca ataccgccat gaacaaattg
      661 attgcccagc tcggtggccc gggaggcgtg acggcttttg cccgcgcgat cggcgatgag
      721 acgtttcgtc tggatcgcac tgaacctacg ctgaataccg ccattcccgg cgacccgaga
      781 gacaccacca cgccgcgggc gatggcgcag acgttgcgtc agcttacgct gggtcatgcg
      841 ctgggcgaaa cccagcgggc gcagttggtg acgtggctca aaggcaatac gaccggcgca
      901 gccagcattc gggccggctt accgacgtcg tggactgtgg gtgataagac cggcagcggc
      961 ggctacggca ccaccaatga tattgcggtg atctggccgc agggtcgtgc gccgctggtt
     1021 ctggtgacct attttaccca gccgcaacag aacgcagaga gccgccgcga tgtgctggct
     1081 tcagcggcga gaatcatcgc cgaagggctg taactggttt tgttgaataa atcgaacttt
     1141 tgctgagttg aaggatcaga tcacgtatct tcccgacaac gcagaccgtt ccgtggcaaa
     1201 gcaaaagttc aaaatcacca actggcccac ctacaataaa gccctcatca accgtggctc
     1261 cataactttc tggctggatg atgaagctat tcaggcctgg tatgagtcag caacaccttc
     1321 ttcacgaggc agacctcagc gctattctga ccttgccatc acgactgtgc tggtcattaa
     1381 acgcgtattc aggctgaccc tgcgcgctgc gcagggcttt attgattcca ttttttctct
     1441 gatgaacgtt ccgctacgct gcccggatta cagctgtgtc agcaggcggg caaagtcggt
     1501 taatatcagt ttcaaaacgc ccacccgggg tgaaatcgca cacctggtaa ttgattccac
     1561 cgggctgaag gtcttcggtg aaggcgagtg gaaagtcaaa aagcatggcc aggaacgccg
     1621 tcgtatatgg cgtaagctgc atctggcagt tgacagtaaa acacatgaaa tcatctgcgc
     1681 tgacctgtcg ctgaacaacg ttacggactc agaggccttc cccgggttaa tccggcaaac
     1741 ccaccggaaa atcaggtcag ccgccgccga tggagcttac gatacccggc tctgtcacga
     1801 tgaactgcgg cgtaagaaaa tcagcgcgct tatcccgccc cgaaaaggtg cgggttactg
     1861 gcccggtgaa tatgcagacc gtaaccgtgc agtggctaat cagcgaatga ccgggagtaa
     1921 tgcgcggtgg aaatggacaa cagattacaa ccgtcgctcg atagcggaaa cggcgatgta
     1981 ccgggtaaaa cagctgttcg ggggttcact gacgctgcgt gactacgatg gtcaggttgc
     2041 ggaggctatg gccctggtac gagcgctgaa caaaatgacg aaagcaggta tgcctgaaag
     2101 cgtgcgtatt gcctgaaaac acaacccgct acgggggaga cttacccgaa atctgattta
     2161 ttcaacaaag ccacgcgctg gacacaccac gatgacagcg gttttatccc ttacgccatc
     2221 accaccggcc ttgattacga ggcgatgacc gaacagcgca aaggctatga gaactttgtt
     2281 tatcagaacg gcgtttacga tctcggcacc aaaggtgata tgcgacgtaa cgaacgtaac
     2341 ctgatgtgga acctcgatcc ctacattcaa acgcactggc agctcacctc agccctgggg
     2401 ctggacgccg gggcgcgcta tagctcggta tggtttgatt ccaacgatcg ttatgttgta
     2461 ggtaaaaatg gtgatgatag cggcgaagcg agctatcaca agtggcttcc ggcggcagcg
     2521 ctgaagtatg ccatgacgcc gcagtggaac gtttacctct ctgccgggag gggatttgaa
     2581 acgccgacca ttaacgagct gtcctatcgt cccgatggtc agtccgggct taactttgct
     2641 ctgcaaccgg ccactaacac caccgttgaa gcaggcagta aatggcaggt gggcaccggg
     2701 atggcgagcc tgagcgtctt caatacggac acgaaaaatg aaattgtcgt tgccgccagc
     2761 gacaatggcc gtacgtccta tcagaacgcg ggcaagacgc gccgccgcgg ggtcgaactg
     2821 ggatgggatc agcaatttgc gcccgcctgg cgcgccaaac tggcgtggac gctgctggat
     2881 gcgacctacc gtgaaaacgc cggcgatgct attcagagcg gcaatcggat cccggggatt
     2941 gcacgtaata gcgcctatgc ctcctttggc tgggtgccgg aagagggatg gtacgctggc
     3001 gctgaagtcc gctatatgag cgatattcag gtcaacgatg ccaatagcga gcaggctcca
     3061 gcctatacgg tgacggcact gaataccggc tataaatatg tgctagataa ctggaccgtc
     3121 gacctctata cgcgcgttga taacctgttt gataagcgct acgtcggctc tgttatcgtc
     3181 aacgagagca acggacgcta ttatgaaccg gcgccgggcc gtaactatgg cgttgggcta
     3241 tcggtcagct atcagtttga ttcgctgtaa caatacggct gccggagagt gctattgagg
     3301 tgaggctccg gcagcgggta aaggcgttga ttcctggtaa aagacgggta aggcgaaatg
     3361 tccagggcca ggcaaattca cgatactgat agtgctgacg tatcagcgct gcatgctcac
     3421 ggcgggtatt ttccctctga ccgtattctg caagaacggt gatatcacga atcccgagct
     3481 gtctggcggt aaaatgccgg acgccggaag gaatatgatt catatcggtg aggaaggtgc
     3541 ccagaaaacg gacacatcca atttgcaggg caatgcccag acggttgtga tcacctctgc
     3601 tttttccgat aaattccttg tctgcttcat caaggtgaaa atatcgggca ctgttgcaaa
     3661 tagtcggtgg tgataaactt
//
LOCUS       DAHHTG010000418         3680 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.guided.245, whole genome shotgun sequence.
ACCESSION   DAHHTG010000418 DAHHTG010000000
VERSION     DAHHTG010000418.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3680)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3680)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3680
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.guided.245"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            238..1113
                     /locus_tag="NBI74_004848"
     CDS             238..1113
                     /locus_tag="NBI74_004848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032492044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A extended-spectrum beta-lactamase
                     CTX-M-27"
                     /protein_id="HCD1935030.1"
                     /translation="MVTKRVQRMMFAAAACIPLLLGSAPLYAQTSAVQQKLAALEKSS
                     GGRLGVALIDTADNTQVLYRGDERFPMCSTSKVMAAAAVLKQSETQKQLLNQPVEIKP
                     ADLVNYNPIAEKHVNGTMTLAELSAAALQYSDNTAMNKLIAQLGGPGGVTAFARAIGD
                     ETFRLDRTEPTLNTAIPGDPRDTTTPRAMAQTLRQLTLGHALGETQRAQLVTWLKGNT
                     TGAASIRAGLPTSWTVGDKTGSGGYGTTNDIAVIWPQGRAPLVLVTYFTQPQQNAESR
                     RDVLASAARIIAEGL"
     gene            1148..2116
                     /locus_tag="NBI74_004849"
     CDS             1148..2116
                     /locus_tag="NBI74_004849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5-like element IS903B family transposase"
                     /protein_id="HCD1935031.1"
                     /translation="MKDQITYLPDNADRSVAKQKFKITNWPTYNKALINRGSITFWLD
                     DEAIQAWYESATPSSRGRPQRYSDLAITTVLVIKRVFRLTLRAAQGFIDSIFSLMNVP
                     LRCPDYSCVSRRAKSVNISFKTPTRGEIAHLVIDSTGLKVFGEGEWKVKKHGQERRRI
                     WRKLHLAVDSKTHEIICADLSLNNVTDSEAFPGLIRQTHRKIRSAAADGAYDTRLCHD
                     ELRRKKISALIPPRKGAGYWPGEYADRNRAVANQRMTGSNARWKWTTDYNRRSIAETA
                     MYRVKQLFGGSLTLRDYDGQVAEAMALVRALNKMTKAGMPESVRIA"
     gene            2173..3270
                     /locus_tag="NBI74_004850"
                     /pseudo
     CDS             2173..3270
                     /locus_tag="NBI74_004850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965681.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
     gene            complement(3338..3610)
                     /locus_tag="NBI74_004851"
                     /pseudo
     CDS             complement(3338..3610)
                     /locus_tag="NBI74_004851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_073984355.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4158 domain-containing protein"
BASE COUNT          912 a          927 c         1057 g          784 t
ORIGIN      
        1 caataaaatt gagtgttgct ctgtggataa cttgcagagt ttattaagta tcattgcagc
       61 aaagatgaaa tcaatgattt atcaaaaatg attgaaaggt ggttgtaaat aatgttacaa
      121 tgtgtgagaa gcagtctaaa ttcttcgtga aatagtgatt tttgaagcta ataaaaaaca
      181 cacgtggaat ttagggaata ctgatgtaac acggattgac cgtattggga gtttgagatg
      241 gtgacaaaga gagtgcaacg gatgatgttc gcggcggcgg cgtgcattcc gctgctgctg
      301 ggcagcgcgc cgctttatgc gcagacgagt gcggtgcagc aaaagctggc ggcgctggag
      361 aaaagcagcg gagggcggct gggcgtcgcg ctcatcgata ccgcagataa tacgcaggtg
      421 ctttatcgcg gtgatgaacg ctttccaatg tgcagtacca gtaaagttat ggcggccgcg
      481 gcggtgctta agcagagtga aacgcaaaag cagctgctta atcagcctgt cgagatcaag
      541 cctgccgatc tggttaacta caatccgatt gccgaaaaac acgtcaacgg cacaatgacg
      601 ctggcagaac tgagcgcggc cgcgttgcag tacagcgaca ataccgccat gaacaaattg
      661 attgcccagc tcggtggccc gggaggcgtg acggcttttg cccgcgcgat cggcgatgag
      721 acgtttcgtc tggatcgcac tgaacctacg ctgaataccg ccattcccgg cgacccgaga
      781 gacaccacca cgccgcgggc gatggcgcag acgttgcgtc agcttacgct gggtcatgcg
      841 ctgggcgaaa cccagcgggc gcagttggtt acgtggctca aaggcaatac gaccggcgca
      901 gccagcattc gggccggctt accgacgtcg tggactgtgg gtgataagac cggcagcggc
      961 ggctacggca ccaccaatga tattgcggtg atctggccgc agggtcgtgc gccgctggtt
     1021 ctggtgacct attttaccca gccgcaacag aacgcagaga gccgccgcga tgtgctggct
     1081 tcagcggcga gaatcatcgc cgaagggctg taactggttt tgttgaataa atcgaacttt
     1141 tgctgagttg aaggatcaga tcacgtatct tcccgacaac gcagaccgtt ccgtggcaaa
     1201 gcaaaagttc aaaatcacca actggcccac ctacaataaa gccctcatca accgtggctc
     1261 cataactttc tggctggatg atgaagctat tcaggcctgg tatgagtcag caacaccttc
     1321 ttcacgaggc agacctcagc gctattctga ccttgccatc acgactgtgc tggtcattaa
     1381 acgcgtattc aggctgaccc tgcgcgctgc gcagggcttt attgattcca ttttttctct
     1441 gatgaacgtt ccgctacgct gcccggatta cagctgtgtc agcaggcggg caaagtcggt
     1501 taatatcagt ttcaaaacgc ccacccgggg tgaaatcgca cacctggtaa ttgattccac
     1561 cgggctgaag gtcttcggtg aaggcgagtg gaaagtcaaa aagcatggcc aggaacgccg
     1621 tcgtatatgg cgtaagctgc atctggcagt tgacagtaaa acacatgaaa tcatctgcgc
     1681 tgacctgtcg ctgaacaacg ttacggactc agaggccttc cccgggttaa tccggcaaac
     1741 ccaccggaaa atcaggtcag ccgccgccga tggagcttac gatacccggc tctgtcacga
     1801 tgaactgcgg cgtaagaaaa tcagcgcgct tatcccgccc cgaaaaggtg cgggttactg
     1861 gcccggtgaa tatgcagacc gtaaccgtgc agtggctaat cagcgaatga ccgggagtaa
     1921 tgcgcggtgg aaatggacaa cagattacaa ccgtcgctcg atagcggaaa cggcgatgta
     1981 ccgggtaaaa cagctgttcg ggggttcact gacgctgcgt gactacgatg gtcaggttgc
     2041 ggaggctatg gccctggtac gagcgctgaa caaaatgacg aaagcaggta tgcctgaaag
     2101 cgtgcgtatt gcctgaaaac acaacccgct acgggggaga cttacccgaa atctgattta
     2161 ttcaacaaag ccacgcgctg gacacaccac gatgacagcg gttttatccc ttacgccatc
     2221 accaccggcc ttgattacga ggcgatgacc gaacagcgca aaggctatga gaactttgtt
     2281 tatcagaacg gcgtttacga tctcggcacc aaaggtgata tgcgacgtaa cgaacgtaac
     2341 ctgatgtgga acctcgatcc ctacattcaa acgcactggc agctcacctc agccctgggg
     2401 ctggacgccg gggcgcgcta tagctcggta tggtttgatt ccaacgatcg ttatgttgta
     2461 ggtaaaaatg gtgatgatag cggcgaagcg agctatcaca agtggcttcc ggcggcagcg
     2521 ctgaagtatg ccatgacgcc gcagtggaac gtttacctct ctgccgggag gggatttgaa
     2581 acgccgacca ttaacgagct gtcctatcgt cccgatggtc agtccgggct taactttgct
     2641 ctgcaaccgg ccactaacac caccgttgaa gcaggcagta aatggcaggt gggcaccggg
     2701 atggcgagcc tgagcgtctt caatacggac acgaaaaatg aaattgtcgt tgccgccagc
     2761 gacaatggcc gtacgtccta tcagaacgcg ggcaagacgc gccgccgcgg ggtcgaactg
     2821 ggatgggatc agcaatttgc gcccgcctgg cgcgccaaac tggcgtggac gctgctggat
     2881 gcgacctacc gtgaaaacgc cggcgatgct attcagagcg gcaatcggat cccggggatt
     2941 gcacgtaata gcgcctatgc ctcctttggc tgggtgccgg aagagggatg gtacgctggc
     3001 gctgaagtcc gctatatgag cgatattcag gtcaacgatg ccaatagcga gcaggctcca
     3061 gcctatacgg tgacggcact gaataccggc tataaatatg tgctagataa ctggaccgtc
     3121 gacctctata cgcgcgttga taacctgttt gataagcgct acgtcggctc tgttatcgtc
     3181 aacgagagca acggacgcta ttatgaaccg gcgccgggcc gtaactatgg cgttgggcta
     3241 tcggtcagct atcagtttga ttcgctgtaa caatacggct gccggagagt gctattgagg
     3301 tgaggctccg gcagcgggta aaggcgttga ttcctggtaa aagacgggta aggcgaaatg
     3361 tccagggcca ggcaaattca cgatactgat agtgctgacg tatcagcgct gcatgctcac
     3421 ggcgggtatt ttccctctga ccgtattctg caagaacggt gatatcacga atcccgagct
     3481 gtctggcggt aaaatgccgg acgccggaag gaatatgatt catatcggtg aggaaggtgc
     3541 ccagaaaacg gacacatcca atttgcaggg caatgcccag acggttgtga tcacctctgc
     3601 tttttccgat aaattccttg tctgcttcat caaggtgaaa atatcgggca ctgttgcaaa
     3661 tagtcggtgg tgataaactt
//
LOCUS       DAHHTG010000419         3276 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.guided.258, whole genome shotgun sequence.
ACCESSION   DAHHTG010000419 DAHHTG010000000
VERSION     DAHHTG010000419.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3276)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3276)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3276
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.guided.258"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            245..1060
                     /gene="sul2"
                     /locus_tag="NBI74_004852"
     CDS             245..1060
                     /gene="sul2"
                     /locus_tag="NBI74_004852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonamide-resistant dihydropteroate synthase
                     Sul2"
                     /protein_id="HCD1935032.1"
                     /translation="MNKSLIIFGIVNITSDSFSDGGRYLAPDAAIAQARKLMAEGADV
                     IDLGPASSNPDAAPVSSDTEIARIAPVLDALKADGIPVSLDSYQPATQAYALSRGVAY
                     LNDIRGFPDAAFYPQLAKSSAKLVVMHSVQDGQADRREAPAGDIMDHIAAFFDARIAA
                     LTGAGIKRNRLVLDPGMGFFLGAAPETSLSVLARFDELRLRFDLPVLLSVSRKSFLRA
                     LTGRGPGDVGAATLAAELAAAAGGADFIRTHEPRPLRDGLAVLAALKETARIR"
     gene            1121..1924
                     /locus_tag="NBI74_004853"
                     /note="aph(3'')-Ib"
     CDS             1121..1924
                     /locus_tag="NBI74_004853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010890156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(3'')-Ib"
                     /protein_id="HCD1935033.1"
                     /translation="MNRTNIFFGESHSDWLPVRGGESGDFVFRRGDGHAFAKIAPASR
                     RGELAGERDRLIWLKGRGVACPEVINWQEEQEGACLVITAIPGVPAADLSGADLLKAW
                     PSMGQQLGAVHSLSVDQCPFERRLSRMFGRAVDVVSRNAVNPDFLPDEDKSTPQLDLL
                     ARVERELPVRLDQERTDMVVCHGDPCMPNFMVDPKTLQCTGLIDLGRLGTADRYADLA
                     LMIANAEENWAAPDEAERAFAVLFNVLGIEAPDRERLAFYLRLDPLTWG"
     gene            1924..2760
                     /locus_tag="NBI74_004854"
     CDS             1924..2760
                     /locus_tag="NBI74_004854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000480972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(6)-Id"
                     /protein_id="HCD1935034.1"
                     /translation="MFMPPVFPAHWHVSQPVLIADTFSSLVWKVSLPDGTPAIVKGLK
                     PIKDIADELRGADYLVWRNGRGAVRLLGRENNLMLLEYAGERMLSHIVAEHGDYQATE
                     IAAELMAKLYAASEEPLPSALLPIRDRFAALFQRARDDQNAGCQTDYVHAAIIADQMM
                     SNASELRGLHGDLHHENIMFSSRGWLVIDPVGLVGEVGFGAANMFYDPADRDDLCLDP
                     RRIAQMADAFSRALDVDPRRLLDQAYAYGCLSAAWNADGEEEQRDLAIAAAIKQVRQT
                     SY"
     gene            2814..3050
                     /locus_tag="NBI74_004855"
     CDS             2814..3050
                     /locus_tag="NBI74_004855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000844627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase"
                     /protein_id="HCD1935035.1"
                     /translation="MAGQSLSVGFRPFPQTTPTRATAHERTPQQERQKAAREAERGRE
                     AWTLGQGMKKPVAGCYGRLTRWKGGGDVVYMALL"
     gene            complement(3082..>3276)
                     /locus_tag="NBI74_004856"
     CDS             complement(3082..>3276)
                     /locus_tag="NBI74_004856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000164043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator
                     C-terminal domain-containing protein"
                     /protein_id="HCD1935036.1"
                     /translation="VLEEQAGGTVEQAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVD
                     GLAKRRLVVRNVEGPRKGDD"
BASE COUNT          652 a          937 c          944 g          743 t
ORIGIN      
        1 ctaattggta ataccatgaa aaataccatg ctcagaaaag gcttaacaat attttgaaaa
       61 attgcctact gagcgctgcc gcacagctcc ataggccgct ttcctggctt tgcttccaga
      121 tgtatgctct tctgctcctg cagctaatgg atcaccgcaa acaggttact cgcctgggga
      181 ttccctttcg acccgagcat ccgtatgata ctcatgctcg attattatta ttatagaagc
      241 ccccatgaat aaatcgctca tcattttcgg catcgtcaac ataacctcgg acagtttctc
      301 cgatggaggc cggtatctgg cgccagacgc agccattgcg caggcgcgta agctgatggc
      361 cgagggggca gatgtgatcg acctcggtcc ggcatccagc aatcccgacg ccgcgcctgt
      421 ttcgtccgac acagaaatcg cgcgtatcgc gccggtgctg gacgcgctca aggcagatgg
      481 cattcccgtc tcgctcgaca gttatcaacc cgcgacgcaa gcctatgcct tgtcgcgtgg
      541 tgtggcctat ctcaatgata ttcgcggttt tccagacgct gcgttctatc cgcaattggc
      601 gaaatcatct gccaaactcg tcgttatgca ttcggtgcaa gacgggcagg cagatcggcg
      661 cgaggcaccc gctggcgaca tcatggatca cattgcggcg ttctttgacg cgcgcatcgc
      721 ggcgctgacg ggtgccggta tcaaacgcaa ccgccttgtc cttgatcccg gcatggggtt
      781 ttttctgggg gctgctcccg aaacctcgct ctcggtgctg gcgcggttcg atgaattgcg
      841 gctgcgcttc gatttgccgg tgcttctgtc tgtttcgcgc aaatcctttc tgcgcgcgct
      901 cacaggccgt ggtccggggg atgtcggggc cgcgacactc gctgcagagc ttgccgccgc
      961 cgcaggtgga gctgacttca tccgcacaca cgagccgcgc cccttgcgcg acgggctggc
     1021 ggtattggcg gcgctgaaag aaaccgcaag aattcgttaa ctgcacattc gggatatttc
     1081 tctatattcg cgcttcatca gaaaactgaa ggaacctcca ttgaatcgaa ctaatatttt
     1141 ttttggtgaa tcgcattctg actggttgcc tgtcagaggc ggagaatctg gtgattttgt
     1201 ttttcgacgt ggtgacgggc atgccttcgc gaaaatcgca cctgcttccc gccgcggtga
     1261 gctcgctgga gagcgtgacc gcctcatttg gctcaaaggt cgaggtgtgg cttgccccga
     1321 ggtcatcaac tggcaggagg aacaggaggg tgcatgcttg gtgataacgg caattccggg
     1381 agtaccggcg gctgatctgt ctggagcgga tttgctcaaa gcgtggccgt caatggggca
     1441 gcaacttggc gctgttcaca gcctatcggt tgatcaatgt ccgtttgagc gcaggctgtc
     1501 gcgaatgttc ggacgcgccg ttgatgtggt gtcccgcaat gccgtcaatc ccgacttctt
     1561 accggacgag gacaagagta cgccgcagct cgatcttttg gctcgtgtcg aacgagagct
     1621 accggtgcgg ctcgaccaag agcgcaccga tatggttgtt tgccatggtg atccctgcat
     1681 gccgaacttc atggtggacc ctaaaactct tcaatgcacg ggtctgatcg accttgggcg
     1741 gctcggaaca gcagatcgct atgccgattt ggcactcatg attgctaacg ccgaagagaa
     1801 ctgggcagcg ccagatgaag cagagcgcgc cttcgctgtc ctattcaatg tattggggat
     1861 cgaagccccc gaccgcgaac gccttgcctt ctatctgcga ttggaccctc tgacttgggg
     1921 ttgatgttca tgccgcctgt ttttcctgct cattggcacg tttcgcaacc tgttctcatt
     1981 gcggacacct tttccagcct cgtttggaaa gtttcattgc cagacgggac tcctgcaatc
     2041 gtcaagggat tgaaacctat aaaagacatt gctgatgaac tgcgcggggc cgactatctg
     2101 gtatggcgca atgggagggg agcagtccgg ttgctcggtc gtgagaacaa tctgatgttg
     2161 ctcgaatatg ccggggagcg aatgctctct cacatcgttg ccgagcacgg cgactaccag
     2221 gcgaccgaaa ttgcagcgga actaatggcg aagctgtatg ccgcatctga ggaacccctg
     2281 ccttctgccc ttctcccgat ccgggatcgc tttgcagctt tgtttcagcg ggcgcgcgat
     2341 gatcaaaacg caggttgtca aactgactac gtccacgcgg cgattatagc cgatcaaatg
     2401 atgagcaatg cctcggaact gcgtgggcta catggcgatc tgcatcatga aaacatcatg
     2461 ttctccagtc gcggctggct ggtgatagat cccgtcggtc tggtcggtga agtgggcttt
     2521 ggcgccgcca atatgttcta cgatccggct gacagagacg acctttgtct cgatcctaga
     2581 cgcattgcac agatggcgga cgcattctct cgtgcgctgg acgtcgatcc gcgtcgcctg
     2641 ctcgaccagg cgtacgctta tgggtgcctt tccgcagctt ggaacgcgga tggagaagag
     2701 gagcaacgcg atctagctat cgcggccgcg atcaagcagg tgcgacagac gtcatactag
     2761 atatcaagcg acttctccta tcccctggga acacatcaat ctcaccggag aatatcgctg
     2821 gccaaagcct tagcgtagga ttccgcccct tcccgcaaac gaccccgacc cgcgccacgg
     2881 cccacgagcg gacgccgcag caggagcgcc agaaggccgc cagagaggcc gagcgcggcc
     2941 gtgaggcttg gacgctaggg cagggcatga aaaagcccgt agcgggctgc tacgggcgtc
     3001 tgacgcggtg gaaaggggga ggggatgttg tctacatggc tctgctgtag tgagtgggtt
     3061 gcgctccggc agcggtcctg atcaatcgtc accctttctc ggtccttcaa cgttcctgac
     3121 aacgagcctc cttttcgcca atccatcgac aatcaccgcg agtccctgct cgaacgctgc
     3181 gtccggaccg gcttcgtcga aggcgtctat cgcggcccgc aacagcggcg agagcggagc
     3241 ctgttcaacg gtgccgccgg cctgctcctc aagcac
//
LOCUS       DAHHTG010000420         2307 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Escherichia coli isolate M96258
            SAMN28743260-rid14505903.guided.276, whole genome shotgun sequence.
ACCESSION   DAHHTG010000420 DAHHTG010000000
VERSION     DAHHTG010000420.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743260
            Sequence Read Archive: SRR19441771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2307)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2307)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316767.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316767.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:24:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 83 of 130
            Pseudo Genes (internal stop)      :: 28 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2307
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743260-rid14505903.guided.276"
                     /isolate="M96258"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-23"
     gene            complement(<1..431)
                     /gene="tetR(A)"
                     /locus_tag="NBI74_004857"
     CDS             complement(<1..431)
                     /gene="tetR(A)"
                     /locus_tag="NBI74_004857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000164043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance transcriptional
                     repressor TetR(A)"
                     /protein_id="HCD1935037.1"
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWH
                     FRNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAG
                     TRPGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGA"
     gene            537..1736
                     /gene="tet(A)"
                     /locus_tag="NBI74_004858"
     CDS             537..1736
                     /gene="tet(A)"
                     /locus_tag="NBI74_004858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(A)"
                     /protein_id="HCD1935038.1"
                     /translation="MKPNRPLIVILSTVALDAVGIGLIMPVLPGLLRDLVHSNDVTAH
                     YGILLALYALMQFACAPVLGALSDRFGRRPVLLVSLAGAAVDYAIMATAPFLWVLYIG
                     RIVAGITGATGAVAGAYIADITDGDERARHFGFMSACFGFGMVAGPVLGGLMGGFSPH
                     APFFAAAALNGLNFLTGCFLLPESHKGERRPLRREALNPLASFRWARGMTVVAALMAV
                     FFIMQLVGQVPAALWVIFGEDRFHWDATTIGISLAAFGILHSLAQAMITGPVAARLGE
                     RRALMLGMIADGTGYILLAFATRGWMAFPIMVLLASGGIGMPALQAMLSRQVDEERQG
                     QLQGSLAALTSLTSIVGPLLFTAIYAASITTWNGWAWIAGAALYLLCLPALRRGLWSG
                     AGQRADR"
     gene            complement(1768..>2307)
                     /locus_tag="NBI74_004859"
     CDS             complement(1768..>2307)
                     /locus_tag="NBI74_004859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000058717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HCD1935039.1"
                     /translation="RLMAVLGAICGTAGVALLVLTPNAALDPVGVAAGLAGAVSMAFG
                     TVLTRKWQPPVPLLTFTAWQLAAGGLLLVPVALVFDPPIPMPTGTNVLGLAWLGLIGA
                     GLTYFLWFRGISRLEPTVVSLLGFLSPGTAVLLGWLFLDQTLSALQIIGVLLVIGSIW
                     LGQRSNRTPRARIACRKSP"
BASE COUNT          413 a          724 c          710 g          460 t
ORIGIN      
        1 gccccaacag tgaagtagct gattgtcatc agcgcattga cggcgtcccc ggccgaaaaa
       61 cccgcctcgc agaggaagcg aagctgcgcg tcggccgttt ccatctgcgg tgcgcccggt
      121 cgcgtgccgg catggatgcg cgcgccatcg cggtaggcga gcagcgcctg cctgaagctg
      181 cgggcattcc cgatcagaaa tgagcgccag tcgtcgtcgg ctctcggcac cgaatgcgta
      241 tgattctccg ccagcatggc ttcggccagt gcgtcgagca gcgcccgctt gttcctgaag
      301 tgccagtaaa gcgccggctg ctgaaccccc aaccgttccg ccagtttgcg tgtcgtcaga
      361 ccgtctacgc cgacctcgtt caacaggtcc agggcggcac ggatcactgt attcggctgc
      421 aactttgtca tgcttgacac tttatcactg ataaacataa tatgtccacc aacttatcag
      481 tgataaagaa tccgcgcgtt caatcggacc agcggaggct ggtccggagg ccagacgtga
      541 aacccaacag acccctgatc gtaattctga gcactgtcgc gctcgacgct gtcggcatcg
      601 gcctgattat gccggtgctg ccgggcctcc tgcgcgatct ggttcactcg aacgacgtca
      661 ccgcccacta tggcattctg ctggcgctgt atgcgttgat gcaatttgcc tgcgcacctg
      721 tgctgggcgc gctgtcggat cgtttcgggc ggcggccggt cttgctcgtc tcgctggccg
      781 gcgctgctgt cgactacgcc atcatggcga cggcgccttt cctttgggtt ctctatatcg
      841 ggcggatcgt ggccggcatc accggggcga ctggggcggt agccggcgct tatattgccg
      901 atatcactga tggcgatgag cgcgcgcggc acttcggctt catgagcgcc tgtttcgggt
      961 tcgggatggt cgcgggacct gtgctcggtg ggctgatggg cggtttctcc ccccacgctc
     1021 cgttcttcgc cgcggcagcc ttgaacggcc tcaatttcct gacgggctgt ttccttttgc
     1081 cggagtcgca caaaggcgaa cgccggccgt tacgccggga ggctctcaac ccgctcgctt
     1141 cgttccggtg ggcccggggc atgaccgtcg tcgccgccct gatggcggtc ttcttcatca
     1201 tgcaacttgt cggacaggtg ccggccgcgc tttgggtcat tttcggcgag gatcgctttc
     1261 actgggacgc gaccacgatc ggcatttcgc ttgccgcatt tggcattctg cattcactcg
     1321 cccaggcaat gatcaccggc cctgtagccg cccggctcgg cgaaaggcgg gcactcatgc
     1381 tcggaatgat tgccgacggc acaggctaca tcctgcttgc cttcgcgaca cggggatgga
     1441 tggcgttccc gatcatggtc ctgcttgctt cgggtggcat cggaatgccg gcgctgcaag
     1501 caatgttgtc caggcaggtg gatgaggaac gtcaggggca gctgcaaggc tcactggcgg
     1561 cgctcaccag cctgacctcg atcgtcggac ccctcctctt cacggcgatc tatgcggctt
     1621 ctataacaac gtggaacggg tgggcatgga ttgcaggcgc tgccctctac ttgctctgcc
     1681 tgccggcgct gcgtcgcggg ctttggagcg gcgcagggca acgagccgat cgctgatcgt
     1741 ggaaacgata ggcctatgcc atgcgggtca aggcgacttc cggcaagcta tacgcgccct
     1801 aggagtgcgg ttggaacgtt ggcccagcca gatactcccg atcacgagca ggacgccgat
     1861 gatttgaagc gcactcagcg tctgatccaa gaacaaccat cctagcaaca cggcggtccc
     1921 cgggctgaga aagcccagta aggaaacaac tgtaggttcg agtcgcgaga tcccccggaa
     1981 ccaaaggaag taggttaaac ccgctccgat caggccgagc cacgccaggc cgagaacatt
     2041 ggttcctgta ggcatcggga ttggcggatc aaacactaaa gctactggaa cgagcagaag
     2101 tcctccggcc gccagttgcc aggcggtaaa ggtgagcaga ggcacgggag gttgccactt
     2161 gcgggtcagc acggttccga acgccatgga aaccgccccc gccaggcccg ctgcgacgcc
     2221 gacaggatct agcgctgcgt ttggtgtcaa caccaacagc gccacgcccg cagttccgca
     2281 aatagccccc aggaccgcca tcaatcg
//
